Genetic variation of Prochilodus lineatus (Valenciennes, 1836) from Paraná, Baía, Miranda, and Corumbá rivers, Brazil.
The curimbatá (Prochilodus lineatus) is one of the migratory species in the Paraná River Basin impacted by the construction of dams. Mitochondrial DNA sequences and random amplified polymorphic DNA (RAPD) fragments were used to investigate genetic variability and geographic structure of five populations of curimbatá from the Paraná River Basin. A total of 1815 bp from seven polymerase chain reaction-amplified fragments representing five protein-coding mitochondrial genes were sequenced from 12 individuals. Estimates of nucleotide sequence divergence ranged from 0.00 to 0.95%. A total of 86 RAPD markers from 58 individuals were detected. Results from the Fisher exact test indicated that P. lineatus is not genetically subdivided, although significant differences in the frequencies of a few RAPD fragments were observed. This study provides useful information for stocking and management programs for resource planning of P. lineatus.
Programa de Pós-Graduação e Ecologia de Ambientes Aquáticos Continentais, Núcleo de Pesquisas em Limnologia, Ictiologia e Aquicultura, Universidade Estadual de Maringá, Maringá, PR, Brasil erevaldaves@hotmail.com.
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Revaldaves
Departamento de Biotecnologia, Genética e Biologia Celular, Núcleo de Pesquisas em Limnologia, Ictiologia e Aquicultura, Universidade Estadual de Maringá, Maringá, PR, Brasil.
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Renesto
Department of Biology, University of New Mexico, Albuquerque, NM, USA.
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Gold
D000818:Animals; D001482:Base Composition / Q000235:genetics; D020029:Base Pairing / Q000235:genetics; D001938:Brazil; D060125:Characiformes / Q000235:genetics*; D004272:DNA, Mitochondrial / Q000235:genetics; D014644:Genetic Variation; D005843:Geography; D006239:Haplotypes / Q000235:genetics; D010802:Phylogeny; D019105:Random Amplified Polymorphic DNA Technique; D045483:Rivers
D004272:DNA, Mitochondrial