Optimization of enzyme-assisted improvement of polyphenols and free radical scavenging activity in red rice bran: A statistical and neural network-based approach.
The current study is focused on optimizing the parameters involved in enzymatic processing of red rice bran for maximizing total polyphenol (TP) and free radical scavenging activity (FRSA). The sequential optimization strategies using central composite design (CCD) and artificial neural network (ANN) modeling linked with genetic algorithm (GA) was performed to study the effect of incubation time (60-90 min), xylanase concentration (5-10 mg/g), cellulase concentration (5-10 mg/g) on the response, i.e., total polyphenol and FRSA. The result showed that incubation time has a negative effect on the response, while the square effect of xylanase and cellulase showed positive effect on the response. A maximum TP of 2,761 mg ferulic acid Eq/100 g bran and FRSA of 778.4 mg Catechin Eq/100 g bran was achieved with incubation time (min) = 60.491; xylanase (mg/g) = 5.4633; cellulase (mg/g) = 11.5825. Furthermore, ANN-GA-based optimization showed better predicting capabilities as compared to CCD.
a Department of Grain Science and Technology , CSIR-Central Food Technological Research Institute , Mysore , Karnataka , India.
Ashish A
AA
Prabhu
a Department of Grain Science and Technology , CSIR-Central Food Technological Research Institute , Mysore , Karnataka , India.
A
A
Jayadeep
D001234:Aspergillus niger / Q000201:enzymology; D001713:Biphenyl Compounds / Q000378:metabolism; D002480:Cellulase / Q000378:metabolism; D003373:Coumaric Acids / Q000378:metabolism; D043364:Endo-1,4-beta Xylanases / Q000378:metabolism; D016166:Free Radical Scavengers / Q000378:metabolism* / Q000494:pharmacology; D005609:Free Radicals / Q000378:metabolism; D016571:Neural Networks, Computer; D012275:Oryza / Q000378:metabolism*; D010851:Picrates / Q000378:metabolism; D010936:Plant Extracts / Q000378:metabolism* / Q000494:pharmacology; D059808:Polyphenols / Q000378:metabolism* / Q000494:pharmacology
D001713:Biphenyl Compounds; D003373:Coumaric Acids; D016166:Free Radical Scavengers; D005609:Free Radicals; D010851:Picrates; D010936:Plant Extracts; D059808:Polyphenols; C004999:ferulic acid; C004931:1,1-diphenyl-2-picrylhydrazyl; D002480:Cellulase; D043364:Endo-1,4-beta Xylanases
Artificial neural network-genetic algorithm; free radical scavenging activity; polyphenols; response surface methodology; rice bran