Control of bacterial nitrate assimilation by stabilization of G-quadruplex DNA.
Here we present a chemical-biology study in the model soil bacterium Paracoccus denitrificans, where we show ligand-specific control of nitrate assimilation. Stabilization of a G-quadruplex in the promoter region of the nas genes, encoding the assimilatory nitrate/nitrite reductase system, is achieved using known quadruplex ligands and results in attenuation of gene transcription.
School of Pharmacy, University of East Anglia, Norwich Research Park, NR4 7TJ, UK. z.waller@uea.ac.uk.
Zoë A E
ZA
Waller
Benjamin J
BJ
Pinchbeck
Bhovina Seewoodharry
BS
Buguth
Timothy G
TG
Meadows
David J
DJ
Richardson
Andrew J
AJ
Gates
D004269:DNA, Bacterial / Q000235:genetics / Q000378:metabolism*; D054856:G-Quadruplexes; D008024:Ligands; D009566:Nitrates / Q000737:chemistry / Q000378:metabolism*; D010231:Paracoccus denitrificans / Q000235:genetics / Q000378:metabolism*
D004269:DNA, Bacterial; D008024:Ligands; D009566:Nitrates