Multiplexed droplet Interface bilayer formation.
We present a simple method for the multiplexed formation of droplet interface bilayers (DIBs) using a mechanically operated linear acrylic chamber array. To demonstrate the functionality of the chip design, a lipid membrane permeability assay is performed. We show that multiple, symmetric DIBs can be created and separated using this robust low-cost approach.
Department of Chemistry, Imperial College London, Exhibition Road, South Kensington, London, SW7 2AZ, UK. o.ces@imperial.ac.uk and Institute of Chemical Biology, Imperial College London, Exhibition Road, South Kensington, London, SW7 2AZ, UK. l.barter@imperial.ac.uk.
Nathan E
NE
Barlow
Department of Chemistry, Imperial College London, Exhibition Road, South Kensington, London, SW7 2AZ, UK. o.ces@imperial.ac.uk and Institute of Chemical Biology, Imperial College London, Exhibition Road, South Kensington, London, SW7 2AZ, UK. l.barter@imperial.ac.uk.
Guido
0000-0002-2380-0794
G
Bolognesi
Institute of Chemical Biology, Imperial College London, Exhibition Road, South Kensington, London, SW7 2AZ, UK. l.barter@imperial.ac.uk and Syngenta, Jealott's Hill International Research Centre, Bracknell, Berkshire, RG42 6EY, UK. anthony.flemming@syngenta.com.
Anthony J
AJ
Flemming
Department of Chemistry, Imperial College London, Exhibition Road, South Kensington, London, SW7 2AZ, UK. o.ces@imperial.ac.uk and Institute of Chemical Biology, Imperial College London, Exhibition Road, South Kensington, London, SW7 2AZ, UK. l.barter@imperial.ac.uk.
Nicholas J
NJ
Brooks
Department of Chemistry, Imperial College London, Exhibition Road, South Kensington, London, SW7 2AZ, UK. o.ces@imperial.ac.uk and Institute of Chemical Biology, Imperial College London, Exhibition Road, South Kensington, London, SW7 2AZ, UK. l.barter@imperial.ac.uk.
Laura M C
LM
Barter
Department of Chemistry, Imperial College London, Exhibition Road, South Kensington, London, SW7 2AZ, UK. o.ces@imperial.ac.uk and Institute of Chemical Biology, Imperial College London, Exhibition Road, South Kensington, London, SW7 2AZ, UK. l.barter@imperial.ac.uk.
Oscar
O
Ces