Evaluation of Haemagglutinin Content by RP-HPLC to Generate Pandemic Influenza Vaccine.
The potency of influenza vaccine is determined based on its hemagglutinin (HA) content. In general, single radial immunodiffusion (SRID) assay has been utilized as the standard method to measure HA content. However, preparation of reagents for SRID such as antigen and antibody takes approximately 2~3 months, which causes delays in the development of influenza vaccine. Therefore, quantification of HA content by other alternative methods is required. In this study, we measured HA contents of H1N1 antigen and H1N1 influenza vaccine by reverse phase-high performance liquid chromatography (RP-HPLC) methods. The presence of HA1 and HA2 was investigated by silver staining and Western blot assay. In addition, accuracy and repeatability of HA measurement by RP-HPLC were evaluated. Comparison of HA concentration by SRID and RP-HPLC revealed a precise correlation between the two methods. Our results suggest that RP-HPLC assay can replace SRID in the event of a pandemic flu outbreak for rapid vaccine development.
Biologics Research Division, National Institute of Food and Drug Safety and Evaluation, Chungwon, Korea.
Hyunkyung
H
Kang
Biologics Research Division, National Institute of Food and Drug Safety and Evaluation, Chungwon, Korea.
Hang Sik
HS
Roh
Biologics Research Division, National Institute of Food and Drug Safety and Evaluation, Chungwon, Korea.
Hyemin
H
Song
Biologics Research Division, National Institute of Food and Drug Safety and Evaluation, Chungwon, Korea.
Kwangmoon
K
Lee
Biologics Research Division, National Institute of Food and Drug Safety and Evaluation, Chungwon, Korea.
Seung-Tae
ST
Chung
Biologics Research Division, National Institute of Food and Drug Safety and Evaluation, Chungwon, Korea.
Sang-Ja
SJ
Ban
College of Veterinary Medicine, Chungbuk National University, Cheongju, Korea.
In Pil
IP
Mo
Department of Biomaterials Science, College of Natural Resources & Life Science/Life and Industry Convergence Research Institute, Pusan National University, Miryang, Korea.
Beum-Soo
BS
An
Biologics Research Division, National Institute of Food and Drug Safety and Evaluation, Chungwon, Korea.
Chi-Young
CY
Ahn
Hemagglutinin; Influenza; RP- HPLC; Vaccine