Animal Models to Study AMPK.
AMPK is an evolutionary conserved energy sensor involved in the regulation of energy metabolism. Based on biochemical studies, AMPK has brought much of interest in recent years due to its potential impact on metabolic disorders. Suitable animal models are therefore essential to promote our understanding of the molecular and functional roles of AMPK but also to bring novel information for the development of novel therapeutic strategies. The organism systems include pig (Sus scrofa), mouse (Mus musculus), fly (Drosophila melanogaster), worm (Caenorhabditis elegans), and fish (Danio rerio) models. These animal models have provided reliable experimental evidence demonstrating the crucial role of AMPK in the regulation of metabolism but also of cell polarity, autophagy, and oxidative stress. In this chapter, we update the new development in the generation and application of animal models for the study of AMPK biology. We also discuss recent breakthroughs from studies in mice, flies, and worms showing how AMPK has a primary role in initiating or promoting pathological or beneficial impact on health.
INSERM U1016, Institut Cochin, Paris, France. benoit.viollet@inserm.fr.|CNRS UMR 8104, Paris, France. benoit.viollet@inserm.fr.|Université Paris Descartes, Sorbonne Paris Cité, Paris, France. benoit.viollet@inserm.fr.
Benoit
B
Viollet
INSERM U1016, Institut Cochin, Paris, France.|CNRS UMR 8104, Paris, France.|Université Paris Descartes, Sorbonne Paris Cité, Paris, France.
Marc
M
Foretz
D055372:AMP-Activated Protein Kinases / Q000235:genetics* / Q000378:metabolism; D000818:Animals; D030801:Animals, Genetically Modified; D017173:Caenorhabditis elegans / Q000201:enzymology / Q000235:genetics*; D017124:Conserved Sequence; D004331:Drosophila melanogaster / Q000201:enzymology / Q000235:genetics*; D004734:Energy Metabolism / Q000235:genetics; D005786:Gene Expression Regulation; D006706:Homeostasis; D006801:Humans; D051379:Mice; D023421:Models, Animal; D055503:Protein Multimerization; D021122:Protein Subunits / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction / Q000235:genetics*; D013552:Swine; D015027:Zebrafish / Q000235:genetics* / Q000378:metabolism
D021122:Protein Subunits; D055372:AMP-Activated Protein Kinases
AMPK-activated protein kinase; Animal models; Energy metabolism; Pharmacological drugs; Therapeutics; Transgenic animals