Ethanol production by Escherichia coli from Arundo donax biomass under SSF, SHF or CBP process configurations and in situ production of a multifunctional glucanase and xylanase.
Diluted acid or liquid hot water (LHW) pretreated Arundo donax biomass was converted into ethanol under separated hydrolysis and fermentation (SHF) or simultaneous saccharification and fermentation (SSF) using Escherichia coli as the fermentative organism. Up to 0.26gL-1h-1and 25.0gL-1of ethanol were obtained with diluted acid pretreated biomass under SSF compared to 0.17gL-1h-1and 24gL-1under SHF. LHW pretreated biomass elicited 25% lower yields on average. Saccharification was carried out with Cellic CTec2 cocktail. Alternatively, under a consolidated bioprocess (CBP) where the ethanologenic bacteria was complemented with a novel multifunctional glucanase and xylanase, ethanol concentration was 7.6gL-1and 7.2gL-1after 96h for dilute acid or LHW pretreated biomass, respectively, without any prior saccharification step. According to these results, a bacterial fermentative host combined with in situ enzyme expression can improve ethanol production from A. donax biomass.
Departamento de Bioquímica y Genómica Microbianas, Instituto de Investigaciones Biológicas Clemente Estable, Montevideo, Uruguay. Electronic address: iloaces@iibce.edu.uy.
Inés
I
Loaces
Departamento de Bioquímica y Genómica Microbianas, Instituto de Investigaciones Biológicas Clemente Estable, Montevideo, Uruguay.
Sima
S
Schein
Departamento de Bioquímica y Genómica Microbianas, Instituto de Investigaciones Biológicas Clemente Estable, Montevideo, Uruguay.
Francisco
F
Noya
D018533:Biomass; D044602:Cellulases / Q000378:metabolism; D004926:Escherichia coli / Q000378:metabolism*; D000431:Ethanol / Q000378:metabolism*; D005285:Fermentation; D006868:Hydrolysis; D006109:Poaceae; D014995:Xylosidases / Q000378:metabolism
D000431:Ethanol; D044602:Cellulases; D014995:Xylosidases
Arundo donax; Bioethanol; Consolidated bioprocessing; E. coli; Process assessment