Isolation and characterization of a Brazilian strain of yellow fever virus from an epizootic outbreak in 2009.
During a series of epizootics caused by Yellow fever virus in Brazil between 2007 and 2009, a monkey was found dead (May 2009) in a sylvatic area in the State of Paraná. Brain samples from this animal were used for immunohistochemical analysis and isolation of a wild-type strain of YFV. This viral strain was characterized, and sequence analyzes demonstrated that it is closely related with YFV strains of the recently identified subclade 1E of the South American genotype I. Further characterization included indirect-immunofluorescence of different infected cell lines and analysis of the kinetics of virus replication and infectivity inhibition by type I IFN. The generated data contributes to the knowledge of YFV evolution and phylogeny. Additionally, the reagents generated and characterized during this study, such as a panel of monoclonal antibodies, are useful tools for further studies on YFV. Lastly, this case stresses the importance of yellow fever surveillance through sentinel monkeys.
Laboratório de Virologia Molecular, Instituto Carlos Chagas, FIOCRUZ, Curitiba, PR, Brazil. Electronic address: taissarj@gmail.com.
Taissa Ricciardi
TR
Jorge
Laboratório de Virologia Molecular, Instituto Carlos Chagas, FIOCRUZ, Curitiba, PR, Brazil. Electronic address: anamosimann@fiocruz.br.
Ana Luiza Pamplona
ALP
Mosimann
Pontifícia Universidade Católica do Paraná, Curitiba, Paraná, Brazil. Electronic address: lnno@terra.com.br.
Lucia de
L
Noronha
Secretaria de Saúde do Estado do Paraná, Brazil. Electronic address: angela.maron@gmail.com.
Angela
A
Maron
Laboratório de Virologia Molecular, Instituto Carlos Chagas, FIOCRUZ, Curitiba, PR, Brazil. Electronic address: clsantos@fiocruz.br.
Claudia Nunes
CN
Duarte Dos Santos
D000818:Animals; D001938:Brazil / Q000453:epidemiology; D004196:Disease Outbreaks / Q000662:veterinary*; D005838:Genotype; D000882:Haplorhini; D008992:Monkey Diseases / Q000453:epidemiology / Q000821:virology*; D010802:Phylogeny; D015004:Yellow Fever / Q000662:veterinary* / Q000821:virology; D015005:Yellow fever virus / Q000235:genetics* / Q000302:isolation & purification
Brazil; Epizootic; Wild-type strain; Yellow fever virus