Transfection of the IHH gene into rabbit BMSCs in a simulated microgravity environment promotes chondrogenic differentiation and inhibits cartilage aging.
The effect of overexpressing the Indian hedgehog (IHH) gene on the chondrogenic differentiation of rabbit bone marrow-derived mesenchymal stem cells (BMSCs) was investigated in a simulated microgravity environment. An adenovirus plasmid encoding the rabbit IHH gene was constructed in vitro and transfected into rabbit BMSCs. Two large groups were used: conventional cell culture and induction model group and simulated microgravity environment group. Each large group was further divided into blank control group, GFP transfection group, and IHH transfection group. During differentiation induction, the expression levels of cartilage-related and cartilage hypertrophy-related genes and proteins in each group were determined. In the conventional model, the IHH transfection group expressed high levels of cartilage-related factors (Coll2 and ANCN) at the early stage of differentiation induction and expressed high levels of cartilage hypertrophy-related factors (Coll10, annexin 5, and ALP) at the late stage. Under the simulated microgravity environment, the IHH transfection group expressed high levels of cartilage-related factors and low levels of cartilage hypertrophy-related factors at all stages of differentiation induction. Under the simulated microgravity environment, transfection of the IHH gene into BMSCs effectively promoted the generation of cartilage and inhibited cartilage aging and osteogenesis. Therefore, this technique is suitable for cartilage tissue engineering.
Department of Orthopaedics, Shanghai Tenth People's Hospital, Tongji University, School of Medicine, Shanghai, People's Republic of China.
Peng-Cheng
PC
Liu
Department of Orthopaedics, Shanghai Tenth People's Hospital, Tongji University, School of Medicine, Shanghai, People's Republic of China.|Department of Rehabilitation, Taihe Hospital, Hubei University of Medicine, Shiyan, Hubei, People's Republic of China.
Kuan
K
Liu
Department of Orthopaedics, Shanghai Tenth People's Hospital, Tongji University, School of Medicine, Shanghai, People's Republic of China.
Jun-Feng
JF
Liu
Department of Orthopaedics, Shanghai Tenth People's Hospital, Tongji University, School of Medicine, Shanghai, People's Republic of China.
Kuo
K
Xia
Department of Orthopaedics, Shanghai Tenth People's Hospital, Tongji University, School of Medicine, Shanghai, People's Republic of China.
Li-Yang
LY
Chen
Department of Orthopaedics, Shanghai Tenth People's Hospital, Tongji University, School of Medicine, Shanghai, People's Republic of China.
Xing
X
Wu
D000256:Adenoviridae / Q000378:metabolism; D000375:Aging; D000818:Animals; D017304:Annexin A5 / Q000737:chemistry; D001854:Bone Marrow Cells / Q000166:cytology; D002356:Cartilage / Q000502:physiology*; D002454:Cell Differentiation; D002478:Cells, Cultured; D020219:Chondrogenesis; D003470:Culture Media; D005346:Fibroblast Growth Factors / Q000378:metabolism; D020869:Gene Expression Profiling; D049452:Green Fluorescent Proteins / Q000378:metabolism; D053823:Hedgehog Proteins / Q000378:metabolism*; D006801:Humans; D006984:Hypertrophy; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D010957:Plasmids / Q000378:metabolism; D011817:Rabbits; D015398:Signal Transduction; D023822:Tissue Engineering / Q000379:methods; D014162:Transfection; D014893:Weightlessness
D017304:Annexin A5; D003470:Culture Media; D053823:Hedgehog Proteins; D049452:Green Fluorescent Proteins; D005346:Fibroblast Growth Factors
BMSCs; Gerotarget; Indian hedgehog; cartilage tissue engineering; hedgehog signaling pathway; rotary cell culture system