Probiotic Lactobacillus fermentum UCO-979C biofilm formation on AGS and Caco-2 cells and Helicobacter pylori inhibition.
The ability of the human isolate Lactobacillus fermentum UCO-979C to form biofilm and synthesize exopolysaccharide on abiotic and biotic models is described. These properties were compared with the well-known Lactobacillus casei Shirota to better understand their anti-Helicobacter pylori probiotic activities. The two strains of lactobacilli synthesized exopolysaccharide as detected by the Dubois method and formed biofilm on abiotic and biotic surfaces visualized by crystal violet staining and scanning electron microscopy. Concomitantly, these strains inhibited H. pylori urease activity by up to 80.4% (strain UCO-979C) and 66.8% (strain Shirota) in gastric adenocarcinoma (AGS) cells, but the two species showed equal levels of inhibition (~84%) in colorectal adenocarcinoma (Caco-2) cells. The results suggest that L. fermentum UCO-979C has probiotic potential against H. pylori infections. However, further analyses are needed to explain the increased activity observed against the pathogen in AGS cells as compared to L. casei Shirota.
a Laboratorio de Patogenicidad Bacteriana, Departamento de Microbiología, Facultad de Ciencias Biológicas , Universidad de Concepción , Concepción , Chile.
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Salas-Jara
a Laboratorio de Patogenicidad Bacteriana, Departamento de Microbiología, Facultad de Ciencias Biológicas , Universidad de Concepción , Concepción , Chile.
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EA
Sanhueza
b Departamento de Genética Molecular y Microbiología, Facultad de Ciencias Biológicas , Pontificia Universidad Católica de Chile , Santiago , Chile.
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Retamal-Díaz
a Laboratorio de Patogenicidad Bacteriana, Departamento de Microbiología, Facultad de Ciencias Biológicas , Universidad de Concepción , Concepción , Chile.
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González
c Laboratorio de Investigación de Estructura, Función y Aplicaciones en Biotecnología Ambiental de Biopelículas Microbianas, Centro de Biotecnología , Universidad de Concepción , Concepción , Chile.
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Urrutia
a Laboratorio de Patogenicidad Bacteriana, Departamento de Microbiología, Facultad de Ciencias Biológicas , Universidad de Concepción , Concepción , Chile.
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García
D000898:Antibiosis; D001422:Bacterial Adhesion; D018441:Biofilms / Q000254:growth & development*; D018938:Caco-2 Cells; D003110:Colonic Neoplasms / Q000382:microbiology; D016480:Helicobacter pylori / Q000187:drug effects / Q000254:growth & development*; D006801:Humans; D007780:Lacticaseibacillus casei / Q000378:metabolism / Q000502:physiology; D052197:Limosilactobacillus fermentum / Q000378:metabolism / Q000502:physiology*; D008855:Microscopy, Electron, Scanning; D011135:Polysaccharides, Bacterial / Q000032:analysis / Q000494:pharmacology; D019936:Probiotics / Q000494:pharmacology; D013274:Stomach Neoplasms / Q000382:microbiology
D011135:Polysaccharides, Bacterial
AGS cells; Caco-2 cells; Helicobacter pylori; Lactobacillus fermentum