Rapid diagnosis and differentiation of microbial pathogens in otitis media with a combined Raman spectroscopy and low-coherence interferometry probe: toward in vivo implementation.
We investigate and demonstrate the feasibility of using a combined Raman scattering (RS) spectroscopy and low-coherence interferometry (LCI) probe to differentiate microbial pathogens and improve our diagnostic ability of ear infections [otitis media (OM)]. While the RS probe provides noninvasive molecular information to identify and differentiate infectious microorganisms, the LCI probe helps to identify depth-resolved structural information as well as to guide and monitor positioning of the Raman spectroscopy beam for relatively longer signal acquisition times. A series of phantom studies, including the use of human middle ear effusion samples, were performed to mimic the conditions of in vivo investigations. These were also conducted to validate the feasibility of using this combined RS/LCI probe for point-of-care diagnosis of the infectious pathogen(s) in OM patients. This work establishes important parameters for future in vivo investigations of fast and accurate determination and diagnosis of infectious microorganisms in OM patients, potentially improving the efficacy and outcome of OM treatments, and importantly reducing the misuse of antibiotics in the presence of viral infections.
Beckman Institute for Advanced Science and Technology, 405 North Mathews Avenue, Urbana, Illinois 61801, United States.
Youbo
Y
Zhao
Beckman Institute for Advanced Science and Technology, 405 North Mathews Avenue, Urbana, Illinois 61801, United StatesbUniversity of Illinois at Urbana-Champaign, Department of Bioengineering, 1304 West Springfield Avenue, Urbana, Illinois 61801, United States.
Guillermo L
GL
Monroy
Beckman Institute for Advanced Science and Technology, 405 North Mathews Avenue, Urbana, Illinois 61801, United StatesbUniversity of Illinois at Urbana-Champaign, Department of Bioengineering, 1304 West Springfield Avenue, Urbana, Illinois 61801, United States.
Sixian
S
You
Beckman Institute for Advanced Science and Technology, 405 North Mathews Avenue, Urbana, Illinois 61801, United States.
Ryan L
RL
Shelton
Beckman Institute for Advanced Science and Technology, 405 North Mathews Avenue, Urbana, Illinois 61801, United States.
Ryan M
RM
Nolan
Beckman Institute for Advanced Science and Technology, 405 North Mathews Avenue, Urbana, Illinois 61801, United States.
Haohua
H
Tu
Beckman Institute for Advanced Science and Technology, 405 North Mathews Avenue, Urbana, Illinois 61801, United States.
Eric J
EJ
Chaney
Beckman Institute for Advanced Science and Technology, 405 North Mathews Avenue, Urbana, Illinois 61801, United StatesbUniversity of Illinois at Urbana-Champaign, Department of Bioengineering, 1304 West Springfield Avenue, Urbana, Illinois 61801, United StatescUniversity of Illinois at Urbana-Champaign, Department of Electrical and Computer Engineering, 306 North Wright Street, Urbana, Illinois 61801, United StatesdUniversity of Illinois at Urbana-Champaign, Department of Internal Medicine, 506 South Mathews Avenue, Urbana, Illinois 61801, United States.
Stephen A
SA
Boppart
D004867:Equipment Design; D006801:Humans; D007368:Interferometry / Q000295:instrumentation* / Q000379:methods; D008954:Models, Biological; D058889:Molecular Typing / Q000295:instrumentation* / Q000379:methods; D010033:Otitis Media / Q000175:diagnosis* / Q000382:microbiology; D010034:Otitis Media with Effusion / Q000382:microbiology; D019095:Point-of-Care Systems; D011550:Pseudomonas aeruginosa / Q000737:chemistry / Q000145:classification; D012815:Signal Processing, Computer-Assisted; D013059:Spectrum Analysis, Raman / Q000295:instrumentation* / Q000379:methods; D013296:Streptococcus pneumoniae / Q000737:chemistry / Q000145:classification; D041623:Tomography, Optical Coherence