Removal of mouse ovary fat pad affects sex hormones, folliculogenesis and fertility.
As a fat storage organ, adipose tissue is distributed widely all over the body and is important for energy supply, body temperature maintenance, organ protection, immune regulation and so on. In humans, both underweight and overweight women find it hard to become pregnant, which suggests that appropriate fat storage can guarantee the female reproductive capacity. In fact, a large mass of adipose tissue distributes around the reproductive system both in the male and female. However, the functions of ovary fat pad (the nearest adipose tissue to ovary) are not known. In our study, we found that the ovary fat pad-removed female mice showed decreased fertility and less ovulated mature eggs. We further identified that only a small proportion of follicles developed to antral follicle, and many follicles were blocked at the secondary follicle stage. The overall secretion levels of estrogen and FSH were lower in the whole estrus cycle (especially at proestrus); however, the LH level was higher in ovary fat pad-removed mice than that in control groups. Moreover, the estrus cycle of ovary fat pad-removed mice showed significant disorder. Besides, the expression of FSH receptor decreased, but the LH receptor increased in ovary fat pad-removed mice. These results suggest that ovary fat pad is important for mouse reproduction.
College of Life SciencesQingdao Agricultural University, Qingdao, China.|State Key Laboratory of Stem Cell and Reproductive BiologyInstitute of Zoology, Chinese Academy of Sciences, Beijing, China.|Institute of Reproductive SciencesKey Laboratory of Animal Reproduction and Germplasm Enhancement in Universities of Shandong, College of Animal Science and Technology, Qingdao Agricultural University, Qingdao, China.
Hong-Hui
HH
Wang
Institute of Reproductive SciencesKey Laboratory of Animal Reproduction and Germplasm Enhancement in Universities of Shandong, College of Animal Science and Technology, Qingdao Agricultural University, Qingdao, China.
Qian
Q
Cui
State Key Laboratory of Stem Cell and Reproductive BiologyInstitute of Zoology, Chinese Academy of Sciences, Beijing, China.|Institute of Reproductive SciencesKey Laboratory of Animal Reproduction and Germplasm Enhancement in Universities of Shandong, College of Animal Science and Technology, Qingdao Agricultural University, Qingdao, China.
Teng
T
Zhang
State Key Laboratory of Stem Cell and Reproductive BiologyInstitute of Zoology, Chinese Academy of Sciences, Beijing, China.
Lei
L
Guo
College of Life SciencesQingdao Agricultural University, Qingdao, China.|State Key Laboratory of Stem Cell and Reproductive BiologyInstitute of Zoology, Chinese Academy of Sciences, Beijing, China.|Institute of Reproductive SciencesKey Laboratory of Animal Reproduction and Germplasm Enhancement in Universities of Shandong, College of Animal Science and Technology, Qingdao Agricultural University, Qingdao, China.
Ming-Zhe
MZ
Dong
State Key Laboratory of Stem Cell and Reproductive BiologyInstitute of Zoology, Chinese Academy of Sciences, Beijing, China.
Yi
Y
Hou
State Key Laboratory of Stem Cell and Reproductive BiologyInstitute of Zoology, Chinese Academy of Sciences, Beijing, China.
Zhen-Bo
ZB
Wang
Institute of Reproductive SciencesKey Laboratory of Animal Reproduction and Germplasm Enhancement in Universities of Shandong, College of Animal Science and Technology, Qingdao Agricultural University, Qingdao, China.
Wei
W
Shen
Laboratory for Germ Cell MetabolismCollege of Animal Science and Technology, Qingdao Agricultural University, Qingdao, China sunqy@ioz.ac.cn jun_yu_ma@aliyun.com.
Jun-Yu
JY
Ma
College of Life SciencesQingdao Agricultural University, Qingdao, China sunqy@ioz.ac.cn jun_yu_ma@aliyun.com.|State Key Laboratory of Stem Cell and Reproductive BiologyInstitute of Zoology, Chinese Academy of Sciences, Beijing, China.|Institute of Reproductive SciencesKey Laboratory of Animal Reproduction and Germplasm Enhancement in Universities of Shandong, College of Animal Science and Technology, Qingdao Agricultural University, Qingdao, China.
Qing-Yuan
QY
Sun
D000273:Adipose Tissue / Q000378:metabolism*; D000818:Animals; D004967:Estrogens / Q000097:blood*; D030762:Estrous Cycle / Q000378:metabolism; D005260:Female; D005298:Fertility / Q000502:physiology*; D005640:Follicle Stimulating Hormone / Q000097:blood; D007986:Luteinizing Hormone / Q000097:blood; D051379:Mice; D006080:Ovarian Follicle / Q000254:growth & development* / Q000378:metabolism; D010053:Ovary / Q000378:metabolism*; D010060:Ovulation / Q000502:physiology
D004967:Estrogens; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone
adipose tissue; female fertility; folliculogenesis; ovary fat pad; sex hormones