Viral diagnostic criteria for Feline immunodeficiency virus and Feline leukemia virus infections in domestic cats from Buenos Aires, Argentina.
A cross-sectional study was carried out on cats attending the Small Animal Hospital at the Faculty of Veterinary Sciences of the University of Buenos Aires to assess the prevalence and associated risk factors of Feline immunodeficiency virus (FIV) and Feline leukemia virus (FeLV) in the city of Buenos Aires, Argentina. Blood samples from 255 cats with symptoms compatible with FIV or FeLV infection, collected between 2009 and 2013 were analyzed by serology (immunochromatography, IA) and by hemi-nested PCR (n-PCR). The IA and n-PCR assays showed similar percentages of positivity for FIV while the n-PCR test was more sensitive for FeLV. Differences between the diagnostic tests and their choice according to the age of the animal are discussed. The clinical histories of ninety of the 255 cats showed blood profiles similar to others previously reported and revealed a higher risk of infection in male adult cats with outdoor access.
Cátedra de Virología, Facultad de Ciencias Veterinarias, Universidad de Buenos Aires, Ciudad Autónoma de Buenos Aires, Argentina.
Sabrina
S
Galdo Novo
Cátedra de Virología, Facultad de Ciencias Veterinarias, Universidad de Buenos Aires, Ciudad Autónoma de Buenos Aires, Argentina.
Danilo
D
Bucafusco
Cátedra de Virología, Facultad de Ciencias Veterinarias, Universidad de Buenos Aires, Ciudad Autónoma de Buenos Aires, Argentina.
Leandro M
LM
Diaz
Cátedra de Virología, Facultad de Ciencias Veterinarias, Universidad de Buenos Aires, Ciudad Autónoma de Buenos Aires, Argentina. Electronic address: abratanich@fvet.uba.ar.
Ana Cristina
AC
Bratanich
D000818:Animals; D001118:Argentina / Q000453:epidemiology; D002415:Cats / Q000821:virology; D002846:Chromatography, Affinity / Q000379:methods*; D003430:Cross-Sectional Studies; D004279:DNA, Viral / Q000032:analysis; D016181:Feline Acquired Immunodeficiency Syndrome / Q000175:diagnosis* / Q000453:epidemiology / Q000821:virology; D005260:Female; D016089:Immunodeficiency Virus, Feline / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D005256:Leukemia Virus, Feline / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D016582:Leukemia, Feline / Q000175:diagnosis* / Q000453:epidemiology / Q000821:virology; D008297:Male; D016133:Polymerase Chain Reaction / Q000379:methods*; D015995:Prevalence; D011533:Proviruses / Q000302:isolation & purification; D011933:Reagent Kits, Diagnostic; D012307:Risk Factors; D012680:Sensitivity and Specificity; D014766:Viremia / Q000175:diagnosis* / Q000453:epidemiology / Q000821:virology
D004279:DNA, Viral; D011933:Reagent Kits, Diagnostic
Feline immunodeficiency virus; Feline leukemia virus; Immunochromatography; Inmunocromatografía; PCR; Virus de la inmunodeficiencia felina; Virus de la leucemia felina