Microwave-assisted synthesis, structure and anti-tumor activity of selenized Artemisia sphaerocephala polysaccharide.
In this work, selenylation of Artemisia sphaerocephala polysaccharide (SeASPMW) was studied by using H2SeO3/HNO3/BaCl2reaction system in microwave field. SeASPMWexhibited the Se content range of 111-264μg/g with high yields (72.1-94.9%).13C NMR results indicated that the weak C-6 substitution was occurred. The decrease (from 7.348×104g/mol to 1.736-4.667×104g/mol) in weight average molecular mass (MW) of SeASPMWwas observed in size exclusion chromatography combined with multi angle laser light scattering (SEC-MALLS) analysis. SeASPMWexhibited a more rigid solution conformation which might be due to the degradation of polysaccharide chains in acidic reaction reagent. This was also supported by atomic force microscopy (AFM) result that SeASPMWshowed short chains and island-like topography. In anti-tumor activity assays, SeASPMW6exhibited the inhibition rates of 32.381% and 39.776% against human non-small cell lung cancer cell line (H1650) at the concentration of 100 and 200μg/mL, respectively. The relatively weak inhibition effect of SeASPMWwas not related to cell apoptosis and cell cycle arrest, suggesting Se content might be a key factor to influence the anti-tumor activities of selenized polysaccharides in vitro.
College of Life Science, Northwest Normal University, Lanzhou 730070, People's Republic of China; Bioactive Products Engineering Research Center For Gansu Distinctive Plants, Northwest Normal University, Lanzhou 730070, People's Republic of China. Electronic address: nuno-vai@163.com.
Junlong
J
Wang
College of Life Science, Northwest Normal University, Lanzhou 730070, People's Republic of China. Electronic address: 13060729@qq.com.
Xiaoping
X
Yang
College of Life Science, Northwest Normal University, Lanzhou 730070, People's Republic of China. Electronic address: wyq127127@163.com.
Aijuan
A
Bao
College of Life Science, Northwest Normal University, Lanzhou 730070, People's Republic of China. Electronic address: xlliu@163.com.
Xinlong
X
Liu
College of Life Science, Northwest Normal University, Lanzhou 730070, People's Republic of China. Electronic address: 1622081261@qq.com.
Junyuan
J
Zeng
College of Life Science, Northwest Normal University, Lanzhou 730070, People's Republic of China. Electronic address: xrliu@sina.com.
Xiurong
X
Liu
College of Life Science, Northwest Normal University, Lanzhou 730070, People's Republic of China; Bioactive Products Engineering Research Center For Gansu Distinctive Plants, Northwest Normal University, Lanzhou 730070, People's Republic of China. Electronic address: yaojiani@nwnu.edu.cn.
Jian
J
Yao
College of Life Science, Northwest Normal University, Lanzhou 730070, People's Republic of China; Bioactive Products Engineering Research Center For Gansu Distinctive Plants, Northwest Normal University, Lanzhou 730070, People's Republic of China; Key Laboratory of Eco-Environment-Related Polymer Materials Ministry of Education, College of Chemistry and Chemical Engineering, Northwest Normal University, Lanzhou 730070, People's Republic of China. Electronic address: nuno-vai@hotmail.com.cn.
Ji
J
Zhang
Key Laboratory of Eco-Environment-Related Polymer Materials Ministry of Education, College of Chemistry and Chemical Engineering, Northwest Normal University, Lanzhou 730070, People's Republic of China. Electronic address: leiziq@nwnu.edu.cn.
Ziqiang
Z
Lei
D017304:Annexin A5 / Q000378:metabolism; D000972:Antineoplastic Agents, Phytogenic / Q000138:chemical synthesis* / Q000302:isolation & purification / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects; D019306:Artemisia / Q000737:chemistry*; D017609:Barium Compounds / Q000737:chemistry; D002236:Carbohydrate Conformation; D059447:Cell Cycle Checkpoints / Q000187:drug effects; D045744:Cell Line, Tumor; D002712:Chlorides / Q000737:chemistry; D004847:Epithelial Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D005434:Flow Cytometry; D006801:Humans; D008872:Microwaves; D008970:Molecular Weight; D017942:Nitric Acid / Q000737:chemistry; D010936:Plant Extracts / Q000737:chemistry*; D011134:Polysaccharides / Q000138:chemical synthesis* / Q000302:isolation & purification / Q000494:pharmacology; D011419:Propidium / Q000737:chemistry; D020887:Selenious Acid / Q000737:chemistry*
D017304:Annexin A5; D000972:Antineoplastic Agents, Phytogenic; D017609:Barium Compounds; D002712:Chlorides; D010936:Plant Extracts; D011134:Polysaccharides; C024986:barium chloride; D011419:Propidium; D017942:Nitric Acid; D020887:Selenious Acid
Anti-tumor activity; Microwave assisted synthesis; Selenylation