Stimulus-dependent dissociation between XB130 and Tks5 scaffold proteins promotes airway epithelial cell migration.
Repair of airway epithelium after injury requires migration of neighboring epithelial cells to injured areas. However, the molecular mechanisms regulating airway epithelial cell migration is not well defined. We have previously shown that XB130, a scaffold protein, is required for airway epithelial repair and regeneration in vivo, and interaction between XB130 and another scaffold protein, Tks5, regulates cell proliferation and survival in human bronchial epithelial cells. The objective of the present study was to determine the role of XB130 and Tks5 interaction in airway epithelial cell migration. Interestingly, we found that XB130 only promotes lateral cell migration, whereas, Tks5 promotes cell migration/invasion via proteolysis of extracellular matrix. Upon stimulation with EGF, PKC activator phorbol 12, 13-dibutyrate or a nicotinic acetylcholine receptor ligand, XB130 and Tks5 translocated to the cell membrane in a stimulus-dependent manner. The translocation and distribution of XB130 is similar to lamellipodial marker, WAVE2; whereas Tks5 is similar to podosome marker, N-WASP. Over-expression of XB130 or Tks5 alone enhances cell migration, whereas co-expression of both XB130 and Tks5 inhibits cell migration processes and signaling. Furthermore, XB130 interacts with Rac1 whereas Tks5 interacts with Cdc42 to promote Rho GTPase activity. Our results suggest that dissociation between XB130 and Tks5 may facilitate lateral cell migration via XB130/Rac1, and vertical cell migration via Tks5/Cdc42. These molecular mechanisms will help our understanding of airway epithelial repair and regeneration.
Institute of Medical Science, Faculty of Medicine, University of Toronto, Toronto, Canada.|Latner Thoracic Surgery Research Laboratories, Toronto General Research Institute, University Health Network, Toronto, Canada.
Serisha
S
Moodley
Latner Thoracic Surgery Research Laboratories, Toronto General Research Institute, University Health Network, Toronto, Canada.
Mathieu
M
Derouet
Latner Thoracic Surgery Research Laboratories, Toronto General Research Institute, University Health Network, Toronto, Canada.
Xiao Hui
XH
Bai
Advanced Optical Microscopy Facility, UHN, Toronto, Canada.
Feng
F
Xu
Institute of Medical Science, Faculty of Medicine, University of Toronto, Toronto, Canada.|Keenan Research Centre for Biomedical Science, St. Michael's Hospital, Toronto, Canada.
Andras
A
Kapus
Institute of Medical Science, Faculty of Medicine, University of Toronto, Toronto, Canada.|Sunnybrook Research Institute, Sunnybrook Health Sciences Centre, Toronto, Canada.
Burton B
BB
Yang
Institute of Medical Science, Faculty of Medicine, University of Toronto, Toronto, Canada.|Latner Thoracic Surgery Research Laboratories, Toronto General Research Institute, University Health Network, Toronto, Canada.
Mingyao
M
Liu
D048868:Adaptor Proteins, Signal Transducing / Q000235:genetics / Q000378:metabolism*; D033942:Adaptor Proteins, Vesicular Transport / Q000235:genetics / Q000378:metabolism*; D015415:Biomarkers; D002460:Cell Line; D002465:Cell Movement / Q000235:genetics; D004847:Epithelial Cells / Q000378:metabolism*; D005109:Extracellular Matrix / Q000378:metabolism; D006801:Humans; D000069261:Podosomes / Q000235:genetics / Q000378:metabolism; D021381:Protein Transport; D011554:Pseudopodia / Q000235:genetics / Q000378:metabolism; D020545:Respiratory Mucosa / Q000378:metabolism*; D051316:Wiskott-Aldrich Syndrome Protein / Q000378:metabolism; D051300:Wiskott-Aldrich Syndrome Protein Family / Q000378:metabolism
C521126:AFAP1L2 protein, human; D048868:Adaptor Proteins, Signal Transducing; D033942:Adaptor Proteins, Vesicular Transport; D015415:Biomarkers; C478582:SH3PXD2A protein, human; D051316:Wiskott-Aldrich Syndrome Protein; D051300:Wiskott-Aldrich Syndrome Protein Family
Pathology Section; SH3PXD2A; actin filament associate protein 1-like 2; lamellipodia; lung repair; podosomes