Effects of biochars and MWNTs on biodegradation behavior of atrazine by Acinetobacter lwoffii DNS32.
Whether the biodegradation of atrazine by Acinetobacter lwoffii DNS32 can have a difference in the presence of two representative carbon materials (CMs), namely, biochars (BCs) and carbon nanotubes (CNTs) is explored, through investigating the influence of CMs on the biodegradation rate, the viability of bacteria and the expression of atrazine genes in aqueous medium. Multi-walled carbon nanotubes (MWNTs), biochars resulted from corn straws (C-BCs) and that made from banana peels (B-BCs) were chosen as the examples. Compared to the control in the absence of C-BCs, B-BCs and MWNTs, the biodegradation efficiencies decrease from 95.3%, 101.8% and 94.8% to 82.6%, 41.8% and 31.1% as the concentrations of these materials increase from 10 to 100mg/L, indicating that BCs have relatively lower toxicity on the biodegradation of atrazine than CNTs, which are agreement with the results of bacterial viability. Transmission electron microscope (TEM) images of Acinetobacter lwoffii DNS32 cells exposure to CMs at 50mg/L show that the cell membrane can be destroyed at different levels after being exposed to various CMs, suggesting that the damage to the cell membrane induced by CMs is a substantial factor leading to the inactivation of bacteria, further decreasing the degradation rate and efficiency of bacteria. The enhanced bacterial growth and the up-regulation of degradation genes can stimulate the degradation rate to pre-adsorbed atrazine on the CMs. This study suggests that biodegradation of atrazine associated with CMs may depend on the carbon composition, structure and CM concentrations. The innovation point of this report is to compare the effects of biochars and CNTs on the degradation rate and activity of Acinetobacter lwoffii DNS32 and may help to further understand the environment effects of CMs.
School of Resources & Environment, Northeast Agricultural University, Harbin, 150030, China; College of Science, Northeast Agricultural University, Harbin 150030, China.
Fan
F
Yang
School of Resources & Environment, Northeast Agricultural University, Harbin, 150030, China.
Qun
Q
Jiang
School of Resources & Environment, Northeast Agricultural University, Harbin, 150030, China.
Moran
M
Zhu
School of Resources & Environment, Northeast Agricultural University, Harbin, 150030, China.
Lulu
L
Zhao
School of Resources & Environment, Northeast Agricultural University, Harbin, 150030, China. Electronic address: zhangyinghr@hotmail.com.
Ying
Y
Zhang
D000150:Acinetobacter / Q000378:metabolism*; D001280:Atrazine / Q000378:metabolism*; D001673:Biodegradation, Environmental; D002606:Charcoal / Q000737:chemistry*; D037742:Nanotubes, Carbon / Q000737:chemistry*
D037742:Nanotubes, Carbon; C540010:biochar; D002606:Charcoal; D001280:Atrazine
Atrazine; Biodegradation; Carbon materials; Gene expression; Viability