Genome sequencing, assembly, annotation and analysis ofStaphylococcus xylosusstrain DMB3-Bh1 reveals genes responsible for pathogenicity.
BACKGROUND
Staphylococcus xylosusis coagulase-negative staphylococci (CNS), found occasionally on the skin of humans but recurrently on other mammals. Recent reports suggest that this commensal bacterium may cause diseases in humans and other animals. In this study, we present the first report of whole genome sequencing ofS. xylosusstrain DMB3-Bh1, which was isolated from the stool of a mouse.


RESULTS
The draft genome ofS. xylosusstrain DMB3-Bh1 consisted of 2,81,0255 bp with G+C content of 32.7 mol%, 2623 predicted coding sequences (CDSs) and 58 RNAs. The final assembly contained 12 contigs of total size 2,81,0255 bp with N50 contig length of 4,37,962 bp and the largest contig assembled measured 7,61,338 bp. Further, an interspecies comparative genomic analysis through rapid annotation using subsystem technology server was achieved withStaphylococcus aureusRF122 that revealed 36 genes having similarity withS. xylosusDMB3-Bh1. 35 genes encoded for virulence, disease and defense and 1 gene encoded for phages, prophages and transposable elements.


CONCLUSIONS
These results suggest co linearity in genes betweenS. xylosusDMB3-Bh1 andS. aureusRF122 that contribute to pathogenicity and might be the result of horizontal gene transfer. The study indicates thatS. xylosusDMB3-Bh1 may be a potential emerging pathogen for rodents.
Microbial Type Culture Collection and Gene Bank (MTCC), CSIR-Institute of Microbial Technology, Sector 39-A, Chandigarh, 160036 India.
Gurwinder
G
Kaur
Microbial Type Culture Collection and Gene Bank (MTCC), CSIR-Institute of Microbial Technology, Sector 39-A, Chandigarh, 160036 India.
Amit
A
Arora
Immunology Laboratory, CSIR-Institute of Microbial Technology, Sector 39-A, Chandigarh, 160036 India.
Sathyaseelan
S
Sathyabama
Immunology Laboratory, CSIR-Institute of Microbial Technology, Sector 39-A, Chandigarh, 160036 India.
Nida
N
Mubin
Immunology Laboratory, CSIR-Institute of Microbial Technology, Sector 39-A, Chandigarh, 160036 India.
Sheenam
S
Verma
Microbial Type Culture Collection and Gene Bank (MTCC), CSIR-Institute of Microbial Technology, Sector 39-A, Chandigarh, 160036 India.
Shanmugam
S
Mayilraj
Immunology Laboratory, CSIR-Institute of Microbial Technology, Sector 39-A, Chandigarh, 160036 India.
Javed N
JN
Agrewala
CDSs; Coagulase-negative staphylococci; Disease and defense; EzTaxon; Rapid annotation using subsystem technology (RAST); Staphylococcus aureus; Virulence