Expression and clinical significance of obesity-associated gene STEAP4 in obese children.
The aim of this study was to investigate the expression and clinical significance of the obesity-associated gene STEAP4 in obese children. Fifty-three obese children and 33 children with a standard body weight (control) from our hospital were recruited to this study. The expression of STEAP4 mRNA and protein in the adipose tissue were detected by reverse transcriptase polymerase chain reaction and enzyme-linked immunosorbent assay, respectively, in order to analyze the relationship between STEAP4 mRNA and protein levels and blood pressure, blood lipid profile, blood glucose levels, and inflammation in obese children. Obese children showed significantly lower levels of STEAP4 mRNA and protein in the adipose tissue compared to the control subjects (P < 0.05). The obese subjects exhibited significantly higher diastolic blood pressure (DBP), systolic blood pressure (SBP), total cholesterol (TC), triglyceride (TG), low-density lipoprotein (LDL), fasting plasma glucose (FPG), interleukin (IL)-6, and tumor necrosis factor (TNF)-α levels, and a significantly lower high-density lipoprotein (HDL) level, compared to the control subjects (P < 0.05). Correlation analysis revealed that STEAP4 expression was negatively correlated with the DBP, SBP, TC, TG, LDL, FPG, IL-6, and TNF-α levels, and was positively correlated with the HDL level (P < 0.05). In conclusion, the expression of STEAP4 was significantly downregulated in the adipose tissue of obese children and was closely related to the blood pressure, blood lipid, blood glucose, and inflammation in these patients; therefore, these results could provide a theoretical basis for the treatment of childhood obesity.
Department of Emergency Medicine, Children's Hospital of Kaifeng City, Kaifeng, China huiminxucn@163.com.
H M
HM
Xu
Department of Hemopathology, Children's Hospital of Kaifeng City, Kaifeng, China.
Y Z
YZ
Cui
Department of Neonatology, Children's Hospital of Kaifeng City, Kaifeng, China.
W G
WG
Wang
Department of Neonatology, Children's Hospital of Kaifeng City, Kaifeng, China.
H X
HX
Cheng
Department of Endocrinology, Children's Hospital of Kaifeng City, Kaifeng, China.
Y J
YJ
Sun
Department of Infectious Diseases, Children's Hospital of Kaifeng City, Kaifeng, China.
H Y
HY
Zhao
Department of Emergency Medicine, Children's Hospital of Kaifeng City, Kaifeng, China.
Y Q
YQ
Yan
D017667:Adipocytes / Q000378:metabolism / Q000473:pathology; D001786:Blood Glucose / Q000378:metabolism; D001794:Blood Pressure; D016022:Case-Control Studies; D002648:Child; D002675:Child, Preschool; D015536:Down-Regulation / Q000235:genetics; D005260:Female; D005786:Gene Expression Regulation; D006801:Humans; D015850:Interleukin-6 / Q000378:metabolism; D008055:Lipids / Q000097:blood; D008297:Male; D008565:Membrane Proteins / Q000235:genetics* / Q000378:metabolism; D009765:Obesity / Q000097:blood / Q000235:genetics* / Q000503:physiopathology; D010088:Oxidoreductases / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
D001786:Blood Glucose; C508600:IL6 protein, human; D015850:Interleukin-6; D008055:Lipids; D008565:Membrane Proteins; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha; D010088:Oxidoreductases; C503310:STEAP4 protein, human