Hollow fiber-stir bar sorptive extraction and microwave assisted derivatization of amino acids in biological matrices.
A kind of solid phase microextraction configuration combining the principles of hollow fiber solid phase microextraction (HF-SPME) and stir bar sorptive extraction (SBSE) is presented. The main feature of HF-SBSE is the use of microporous hollow fiber acting as the carrier and filter, while a thin stainless steel wire and silica microspheres in the lumen of hollow fiber respectively acting as the magnetic stirrer and the dispersed sorbents for the collection and extraction of the target analytes, thus affording extraction process like SBSE. Moreover, the prepared hollow fiber stir bar was applied to direct microextraction and microwave assisted derivatization with N,O-Bis(trimethylsilyl)trifluroacetamide (BSTFA) of four amino acids in rats' urine and cerebrospinal fluid followed by gas chromatography mass spectrometric analysis. The limits of detection for four amino acids were found to be in the range of 0.0003-0.017μgmL-1, and all the analytes did not exhibit any lack of fit. The extraction recoveries using HF-SBSE techniques ranged from 71.8% to 102.3%. The results indicated that hollow fiber stir bar sorptive extraction was a promising technique for the enrichment and direct derivatization of analytes extracted from biological matrices without sample clean-up.
Chemical Engineering Institute, Key Laboratory of Environmental Friendly Composite Materials and Biomass Utilization, Northwest University for Nationalities, Lanzhou 730000, China. Electronic address: lj5458325@126.com.
Jia
J
Li
Xiamen Medicine Research Institute, Xiamen 361008, China.
Huan-Yang
HY
Qi
Chemical Engineering Institute, Key Laboratory of Environmental Friendly Composite Materials and Biomass Utilization, Northwest University for Nationalities, Lanzhou 730000, China.
Yan-Bin
YB
Wang
Chemical Engineering Institute, Key Laboratory of Environmental Friendly Composite Materials and Biomass Utilization, Northwest University for Nationalities, Lanzhou 730000, China.
Qiong
Q
Su
Chemical Engineering Institute, Key Laboratory of Environmental Friendly Composite Materials and Biomass Utilization, Northwest University for Nationalities, Lanzhou 730000, China.
Shang
S
Wu
Chemical Engineering Institute, Key Laboratory of Environmental Friendly Composite Materials and Biomass Utilization, Northwest University for Nationalities, Lanzhou 730000, China. Electronic address: wulan@xbmu.edu.cn.
Lan
L
Wu
D000327:Adsorption; D000596:Amino Acids / Q000032:analysis* / Q000134:cerebrospinal fluid / Q000652:urine; D000818:Animals; D008401:Gas Chromatography-Mass Spectrometry; D007202:Indicators and Reagents; D008863:Microspheres; D008872:Microwaves; D051381:Rats; D012015:Reference Standards; D015203:Reproducibility of Results; D052617:Solid Phase Microextraction; D012997:Solvents; D014297:Trimethylsilyl Compounds
D000596:Amino Acids; D007202:Indicators and Reagents; D012997:Solvents; D014297:Trimethylsilyl Compounds; C047270:N,N-bis(trimethylsilyl)-2,2,2-trifluoroacetamide
Amino acids; Biological matrices; Hollow fiber; Microwave assisted derivatization; Silylation; Stir bar sorptive extraction