[The application of yeast hybrid systems in protein interaction analysis].
Yeast hybrid systems have been widely used due to their convenience and low cost. Based on these systems, many methods have been developed to analyze protein-protein, protein-DNA and protein-RNA interactions. In this paper, we are reviewing these different yeast hybrid systems. According to the number of hybrid proteins, yeast hybrid systems can be divided into three categories, yeast one-hybrid, yeast two-hybrid and yeast three-hybrid systems. Alternatively, yeast hybrid systems can be categorized according to the subcellular localization of the protein interaction process in the cell into nuclear protein-protein interactions, cytosol protein-protein interactions and membrane protein-protein interactions. Throughout the review, we focus on the progress and limitations of each yeast hybrid system over the recent years.
Agricultural Crops Molecular Improving Lab, Liaoning Academy of Agricultural Sciences, Shenyang, 110161 China.
Z-X
ZX
Zhu
College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou, 310018 China.
Z-M
ZM
Yu
School of Life Sciences, Gibbet Hill Campus, University of Warwick, Coventry CV4 7AL, United Kingdom.
J L
JL
Taylor
College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou, 310018 China.
Y-H
YH
Wu
College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou, 310018 China.|nijun@hznu.edu.cn.
J
J
Ni
D000818:Animals; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D006801:Humans; D016601:RNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism; D020798:Two-Hybrid System Techniques
D004268:DNA-Binding Proteins; D016601:RNA-Binding Proteins
GAL4; cytosol; interaction; membrane; nuclear; protein; yeast hybrid system