Characterization of the interaction between heme and a parallel G-quadruplex DNA formed from d(TTGAGG).
Structure-function relationships of complexes between heme and G-quadruplex DNAs have attracted interest from researchers in related fields. A carbon monoxide adduct of a complex between heme and a parallel G-quadruplex DNA formed from hexanucleotide d(TTGAGG) (heme-[d(TTGAGG)]4complex) has been characterized using1H NMR spectroscopy, and the obtained results were compared with those for the heme-[d(TTAGGG)]4complex previously studied in order to elucidate the effect of the incorporation of an A-quartet into stacked G-quartets in the 3'-terminal region of the DNA on the structure of the heme-DNA complex. We found that a π-π stacking interaction between the porphyrin moiety of the heme and the 3'-terminal G-quartet of the DNA is affected by the nature of the stacked G-quartets. This finding provides novel insights as to the design of the molecular architecture of a heme-DNA complex. This article is part of a Special Issue entitled "G-quadruplex" Guest Editor: Dr. Concetta Giancola and Dr. Daniela Montesarchio.
Department of Chemistry, University of Tsukuba, Tsukuba 305-8571, Japan.
Tomokazu
T
Shibata
Department of Chemistry, University of Tsukuba, Tsukuba 305-8571, Japan.
Yusaku
Y
Nakayama
Department of Chemistry, University of Tsukuba, Tsukuba 305-8571, Japan.
Yuya
Y
Katahira
Graduate School of Materials Science, Nara Institute of Science and Technology, Ikoma, Nara 630-0192, Japan.
Hulin
H
Tai
Department of Chemistry, University of Tsukuba, Tsukuba 305-8571, Japan.
Yuki
Y
Moritaka
Department of Chemistry, University of Tsukuba, Tsukuba 305-8571, Japan.
Yusuke
Y
Nakano
Department of Chemistry, University of Tsukuba, Tsukuba 305-8571, Japan; Life Science Center of Tsukuba Advanced Research Alliance, University of Tsukuba, Tsukuba 305-8577, Japan. Electronic address: yamamoto@chem.tsukuba.ac.jp.
Yasuhiko
Y
Yamamoto
D001665:Binding Sites; D002248:Carbon Monoxide / Q000737:chemistry / Q000378:metabolism; D054856:G-Quadruplexes; D006418:Heme / Q000737:chemistry* / Q000378:metabolism; D008958:Models, Molecular; D009691:Nucleic Acid Denaturation; D009841:Oligonucleotides / Q000737:chemistry* / Q000378:metabolism; D011485:Protein Binding; D054730:Protein Interaction Domains and Motifs; D066244:Proton Magnetic Resonance Spectroscopy; D013329:Structure-Activity Relationship; D013696:Temperature
D009841:Oligonucleotides; D006418:Heme; D002248:Carbon Monoxide
A-quartet; DNA; G-quartet; Heme; π-π stacking