Citeromyces matritensis M37 is a salt-tolerant yeast that produces ethanol from salted algae.
Algae are referred to as a third-generation biomass for ethanol production. However, salinity treatment is a problem that needs to be solved, because algal hydrolysates often contain high salt. Here, we isolated the salt-tolerant ethanol-producing yeast Citeromyces matritensis M37 from the east coast of Miura Peninsula in Japan. This yeast grew under osmotic stress conditions (20% NaCl or 60% glucose). It produced 6.55 g/L ethanol from YPD medium containing 15% NaCl after 48 h, and the ethanol accumulation was observed even at 20% NaCl. Using salted Undaria pinnatifida (wakame), we obtained 6.33 g/L glucose from approx. 150 g/L of the salted wakame powder with acidic and heat pretreatment followed by enzymatic saccharification, and the ethanol production reached 2.58 g/L for C. matritensis M37. The ethanol concentration was 1.4 times higher compared with that using the salt-tolerant ethanol-producing yeast Zygosaccharomyces rouxii S11.
a Tokyo University of Marine Science and Technology, 4-5-7 Konan, Minato-ku, Tokyo 108-8477, Japan.
Masahiko
M
Okai
a Tokyo University of Marine Science and Technology, 4-5-7 Konan, Minato-ku, Tokyo 108-8477, Japan.
Ayako
A
Betsuno
a Tokyo University of Marine Science and Technology, 4-5-7 Konan, Minato-ku, Tokyo 108-8477, Japan.
Ayaka
A
Shirao
a Tokyo University of Marine Science and Technology, 4-5-7 Konan, Minato-ku, Tokyo 108-8477, Japan.
Nobuo
N
Obara
b Zensho Laboratories of Food Technology, Zensho Holdings Co. Ltd., 2-18-1 Konan, Minato-ku, Tokyo 108-0075, Japan.
Kotaro
K
Suzuki
b Zensho Laboratories of Food Technology, Zensho Holdings Co. Ltd., 2-18-1 Konan, Minato-ku, Tokyo 108-0075, Japan.
Toshinori
T
Takei
b Zensho Laboratories of Food Technology, Zensho Holdings Co. Ltd., 2-18-1 Konan, Minato-ku, Tokyo 108-0075, Japan.
Masachika
M
Takashio
a Tokyo University of Marine Science and Technology, 4-5-7 Konan, Minato-ku, Tokyo 108-8477, Japan.
Masami
M
Ishida
a Tokyo University of Marine Science and Technology, 4-5-7 Konan, Minato-ku, Tokyo 108-8477, Japan.
Naoto
N
Urano
D000431:Ethanol / Q000378:metabolism*; D005285:Fermentation; D007564:Japan; D004718:Saccharomycetales / Q000145:classification / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D012623:Seawater / Q000382:microbiology*; D012965:Sodium Chloride / Q000378:metabolism*
D000431:Ethanol; D012965:Sodium Chloride
Citeromyces matritensis; Undaria pinnatifida; algae; algues; condition à teneur élevée en sel; ethanol production; high salt condition; production d’éthanol