TRPM7is down-regulated in both left atria and left ventricle of ischaemic cardiomyopathy patients and highly related to changes in ventricular function.
OBJECTIVE
The kinase ion channel transient receptor potential melastatin 7 (TRPM7) is considered a modulator of cardiac fibrosis progression; nevertheless, we lack of studies analysing its role in human ischaemic cardiomyopathy (ICM). Our objective was to analyse the expression of genes encoding cardiac ion channels in human ICM, focusing on the alterations in mRNA levels ofTRPM7and its relationship with changes in the ventricular function.


RESULTS
RNA-sequencing was carried out in 13 left ventricular (LV) samples of patients with ICM compared with a control group (n= 10). The analysis revealed a total of 25 ion channel genes differentially expressed. We performed an RTqPCR analysis of theTRPM7mRNA in LV and left atrial samples and found that it was down-regulated in both cavities (-1.43-fold and -1.52-fold, respectively). AtrialTRPM7mRNA levels showed an excellent and inverse relationships with the depressed ejection fraction (r= -0.724,P= 0.042) and with the mitral A wave (r= -0.938,P= 0.006).


CONCLUSIONS
We report the down-regulation ofTRPM7in tissue samples from both left atria and left ventricle in patients with ICM. We found an inverse relationship between both cardiac chambers mRNA levels with LV dysfunction, suggesting an important role of TRPM7 in the left atrial and LV functional depression found in this cardiomyopathy.
Cardiocirculatory Unit Health Research Institute of La Fe University Hospital Valencia Spain.
Ana
A
Ortega
Cardiocirculatory Unit Health Research Institute of La Fe University Hospital Valencia Spain.
Esther
E
Roselló-Lletí
Cardiocirculatory Unit Health Research Institute of La Fe University Hospital Valencia Spain.
Estefanía
E
Tarazón
Cardiocirculatory Unit Health Research Institute of La Fe University Hospital Valencia Spain.
Carolina
C
Gil-Cayuela
Cellular and Molecular Cardiology Research Unit, Department of Cardiology and Institute of Biomedical Research University Clinical Hospital Santiago de Compostela Spain.
Francisca
F
Lago
Cellular and Molecular Cardiology Research Unit, Department of Cardiology and Institute of Biomedical Research University Clinical Hospital Santiago de Compostela Spain.
Jose-Ramón
JR
González-Juanatey
Heart Failure and Transplantation Unit, Cardiology Department La Fe University Hospital Valencia Spain.
Luis
L
Martinez-Dolz
Cardiocirculatory Unit Health Research Institute of La Fe University Hospital Valencia Spain.
Manuel
M
Portolés
Cardiocirculatory Unit Health Research Institute of La Fe University Hospital Valencia Spain.
Miguel
M
Rivera
TRPM7; ischaemic cardiomyopathy; left ventricular dysfunction