Novel folic acid conjugated Fe3O4-ZnO hybrid nanoparticles for targeted photodynamic therapy.
A novel folic acid conjugated core-shell hybrid iron oxide-zinc oxide nanoparticle was developed for applications as a photosensitier (PS) in photodynamic therapy. Photodegradation studies on methylene blue demonstrated significantly enhanced photophysical properties of the produced nano-PSs, due to the charge recombination via electron trapping by dissolved Fe3+. A time and dose dependant toxicity associated with the nano-PSs was observed upon exposure to human epithelial colorectal adenocarcinoma (Caco-2) cells in the dark. UV irradiation of the synthesised nano-PSs resulted in a significant photo-killing effect with drastic reduction in Caco-2 cell viability to as low as 6%. Reduction in viability upon exposure was due fundamentally to cellular interactions with light irradiated PSs as the influence of radiation alone was subtracted. FA conjugation further enhanced the photo-killing effect.
Department of Chemical Engineering, Curtin University, Kent Street, Bentley WA 6102, Australia.
Kunal
K
Patel
School of Pharmacy, CHIRI-Biosciences, Curtin University, Kent Street, Bentley WA 6102, Australia.
Behin Sundara
BS
Raj
School of Pharmacy, CHIRI-Biosciences, Curtin University, Kent Street, Bentley WA 6102, Australia.
Yan
Y
Chen
Department of Chemical Engineering, Curtin University, Kent Street, Bentley WA 6102, Australia. Electronic address: x.lou@curtin.edu.au.
Xia
X
Lou
D000327:Adsorption; D018938:Caco-2 Cells; D002470:Cell Survival; D004305:Dose-Response Relationship, Drug; D005290:Ferric Compounds / Q000737:chemistry*; D005492:Folic Acid / Q000737:chemistry*; D006801:Humans; D007700:Kinetics; D008027:Light; D053768:Metal Nanoparticles / Q000737:chemistry*; D010778:Photochemotherapy / Q000379:methods*; D010782:Photolysis; D017550:Spectroscopy, Fourier Transform Infrared; D014466:Ultraviolet Rays; D015034:Zinc Oxide / Q000737:chemistry*
D005290:Ferric Compounds; C000499:ferric oxide; D005492:Folic Acid; D015034:Zinc Oxide
Cancer targeting; Photo-killing; Photocatalysts; Photodynamic therapy; Photosensitiser