Optimization of Culture Conditions for Growth Associated with Cr(VI) Removal by Wickerhamomyces anomalus M10.
Chromate-resistant microorganisms with the ability of reducing toxic Cr(VI) to less toxic Cr(III), are candidates for bioremediation. An alternative culture medium to reduce Cr(VI) using Wickerhamomyces anomalus M10 was optimized. Using the Plackett-Burman design, it was determined that sucrose, K2HPO4and inoculum size had significant effects on chromate removal (i.e., reduction) at 24 h. Concentrations of these significant factors were adjusted using a complete factorial design. In this case, only the K2HPO4effect was significant at 12 h of culture, with greater Cr(VI) removal at low concentration (1.2 g L-1). The optimum medium was validated at the fermenter scale level. Optimal culture conditions for complete removal of Cr(VI) (1 mM) were 400 rpm agitation and air flow of 1 vvm. Moreover, W. anomalus M10 completely removed consecutively added pulses of Cr(VI) (1 mM). These results show interesting characteristics from the standpoint of biotechnology because the development of a future remediation process using W. anomalus M10 can represent an efficient and highly profitable technology for removing the toxic form of Cr.
PROIMI-CONICET, Av. Belgrano y Caseros (T4001MVB), Tucumán, Argentina. pablomfernandez79@gmail.com.
Pablo Marcelo
PM
Fernández
PROIMI-CONICET, Av. Belgrano y Caseros (T4001MVB), Tucumán, Argentina.
Elías Leonardo
EL
Cruz
PROIMI-CONICET, Av. Belgrano y Caseros (T4001MVB), Tucumán, Argentina.
Silvana Carolina
SC
Viñarta
PROIMI-CONICET, Av. Belgrano y Caseros (T4001MVB), Tucumán, Argentina.|Microbiología Superior, Facultad de Bioquímica, Química y Farmacia, Universidad Nacional de Tucumán, Tucumán, Argentina.
Lucía Inés
LI
Castellanos de Figueroa
D001673:Biodegradation, Environmental; D002857:Chromium / Q000032:analysis*; D003470:Culture Media / Q000737:chemistry*; D010084:Oxidation-Reduction; D004718:Saccharomycetales / Q000378:metabolism*
D003470:Culture Media; D002857:Chromium; C074702:chromium hexavalent ion
Bioreactor; Bioremediation; Cr(VI); Cr(VI)-resistant yeasts; Factorial design; Plackett-Burman