Subsite-specific association of DEAD box RNA helicase DDX60 with the development and prognosis of oral squamous cell carcinoma.
The clinical significance and biological function of DEXD/H box helicase 60 (DDX60) in oral cancer remains unknown. Herein, we evaluated the association of DDX60 expression with tumorigenesis and the prognosis of oral squamous cell carcinoma (OSCC). DDX60 expression was examined by immunohistochemistry on tissue microarray slides of 494 OSCC patients, including 180 buccal mucosal SCC (BMSCC), 241 tongue SCC (TSCC), and 73 lip SCC (LSCC) patients. DDX60 expression was significantly increased in all three subsites of OSCC compared to its expression in tumor adjacent normal tissues. However, its association with tumorigenesis was specific to the oral cavity subsite after the stratification of betel quid chewing, smoking, and drinking. Among OSCC patients, higher levels of DDX60 expression were associated with the male gender, a well-differentiated tumor, advanced stage of disease, and a large tumor size with subsite specific features. LSCC patients with high DDX60 expression levels showed shorter disease-specific survival, particularly those with moderately or poorly differentiated tumors. Additionally, TSCC or OSCC patients with high DDX60 expression showed a poor disease-free survival (DFS), particularly those with moderately or poorly differentiated tumors. Therefore, DDX60 is a novel and unfavorable biomarker for tumorigenesis and prognosis of OSCC in a subsite-specific manner.
Department of Pathology and Laboratory Medicine, Kaohsiung Veterans General Hospital, Kaohsiung, Taiwan.|Department of Optometry, Shu-Zen Junior College of Medicine and Management, Kaohsiung, Taiwan.
Ting-Ying
TY
Fu
Department of Stomatology, Kaohsiung Veterans General Hospital, Kaohsiung, Taiwan.|Department of Dental Technology, Shu-Zen Junior College of Medicine and Management, Kaohsiung, Taiwan.
Chao-Nan
CN
Wu
Department of Pathology and Laboratory Medicine, Kaohsiung Veterans General Hospital, Kaohsiung, Taiwan.
Huei-Cin
HC
Sie
Department of Biological Sciences, National Sun Yat-Sen University, Kaohsiung, Taiwan.
Jiin-Tsuey
JT
Cheng
Department of Otorhinolaryngology-Head & Neck Surgery, Kaohsiung Veterans General Hospital, Kaohsiung, Taiwan.
Yaoh-Shiang
YS
Lin
Department of Medical Education and Research, Kaohsiung Veterans General Hospital, Kaohsiung, Taiwan.
Huei-Han
HH
Liou
Department of Orthopedics, Show Chwan Memorial Hospital, Changhua, Taiwan.|Department of Orthopedics, National Cheng Kung University Hospital, Tainan, Taiwan.
Yu-Kai
YK
Tseng
Department of Medical Education and Research, Kaohsiung Veterans General Hospital, Kaohsiung, Taiwan.
Chih-Wen
CW
Shu
Department of Medical Education and Research, Kaohsiung Veterans General Hospital, Kaohsiung, Taiwan.|Department of Chemical Biology, National Pingtung University of Education, Pingtung, Taiwan.
Kuo-Wang
KW
Tsai
Department of Pathology and Laboratory Medicine, Kaohsiung Veterans General Hospital, Kaohsiung, Taiwan.
Leing-Ming
LM
Yen
Department of Dermatology, Kaohsiung Veterans General Hospital, Kaohsiung, Taiwan.|Department of Nursing, Meiho University, Pingtung, Taiwan.
Hui-Wen
HW
Tseng
Department of Medical Education and Research, Kaohsiung Veterans General Hospital, Kaohsiung, Taiwan.|Institute of Clinical Medicine, National Yang-Ming University, Taipei, Taiwan.|Department of Pharmacology, National Defense Medical Center, Taipei, Taiwan.|Department of Medical Research, China Medical University Hospital, China Medical University, Taichung, Taiwan.
Ching-Jiunn
CJ
Tseng
Department of Medical Education and Research, Kaohsiung Veterans General Hospital, Kaohsiung, Taiwan.|Institute of Biomedical Sciences, National Sun Yat-Sen University, Kaohsiung, Taiwan.
Luo-Ping
LP
Ger
Department of Medical Education and Research, Kaohsiung Veterans General Hospital, Kaohsiung, Taiwan.|Department of Biotechnology, Fooyin University, Kaohsiung, Taiwan.
Pei-Feng
PF
Liu
D014408:Biomarkers, Tumor / Q000235:genetics / Q000378:metabolism*; D063646:Carcinogenesis; D002294:Carcinoma, Squamous Cell / Q000235:genetics / Q000378:metabolism* / Q000401:mortality; D053487:DEAD-box RNA Helicases / Q000235:genetics / Q000378:metabolism*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D008875:Middle Aged; D009062:Mouth Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000401:mortality; D009367:Neoplasm Staging; D009928:Organ Specificity; D011379:Prognosis; D016019:Survival Analysis; D046888:Tissue Array Analysis; D015854:Up-Regulation
D014408:Biomarkers, Tumor; C561888:DDX60 protein, human; D053487:DEAD-box RNA Helicases
DDX60; oral cancer; prognosis; subsite-specific; tumorigenesis