Activation of biologically relevant levels of reactive oxygen species by Au/g-C3N4hybrid nanozyme for bacteria killing and wound disinfection.
As common reactive oxygen species, H2O2is widely used for bacterial inactivation and wound disinfection. However, the concentrations used are always higher than physiological levels, which frequently result in potential toxicity to healthy tissue and even delay wound healing. Here we report highly efficient nanozyme hybrids that are capable of activating biologically relevant concentrations of H2O2for defending bacterial infections. The integration of AuNPs with ultrathin graphitic carbon nitride (g-C3N4) provides excellent peroxidase-activity, which can catalyze the decomposition of H2O2to OH radicals much more efficiently, allowing the use of bio-safety levels of H2O2for the first time. Furthermore, our system not only exhibits striking bactericidal performance against both DR Gram-negative and DR Gram-positive bacteria, but also shows high efficiency in breaking down the existing DR-biofilms and prevented formation of new biofilms in vitro. More importantly, in vivo experiments indicate that our system could significantly prevent bacterial infections and accelerate the healing rate of wounds.
Laboratory of Chemical Biology and State Key Laboratory of Rare Earth Resources Utilization, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, 130022, PR China; University of Chinese Academy of Sciences, Beijing, 100039, PR China.
Zhenzhen
Z
Wang
Laboratory of Chemical Biology and State Key Laboratory of Rare Earth Resources Utilization, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, 130022, PR China.
Kai
K
Dong
Laboratory of Chemical Biology and State Key Laboratory of Rare Earth Resources Utilization, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, 130022, PR China.
Zhen
Z
Liu
Laboratory of Chemical Biology and State Key Laboratory of Rare Earth Resources Utilization, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, 130022, PR China; University of Chinese Academy of Sciences, Beijing, 100039, PR China.
Yan
Y
Zhang
Laboratory of Chemical Biology and State Key Laboratory of Rare Earth Resources Utilization, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, 130022, PR China; University of Chinese Academy of Sciences, Beijing, 100039, PR China.
Zhaowei
Z
Chen
Laboratory of Chemical Biology and State Key Laboratory of Rare Earth Resources Utilization, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, 130022, PR China; University of Chinese Academy of Sciences, Beijing, 100039, PR China.
Hanjun
H
Sun
Laboratory of Chemical Biology and State Key Laboratory of Rare Earth Resources Utilization, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, 130022, PR China. Electronic address: jren@ciac.ac.cn.
Jinsong
J
Ren
Laboratory of Chemical Biology and State Key Laboratory of Rare Earth Resources Utilization, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, 130022, PR China. Electronic address: xqu@ciac.ac.cn.
Xiaogang
X
Qu
D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use; D001419:Bacteria / Q000187:drug effects*; D001424:Bacterial Infections / Q000150:complications / Q000188:drug therapy* / Q000382:microbiology; D018441:Biofilms / Q000187:drug effects; D004203:Disinfection; D006046:Gold / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use; D006108:Graphite / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use; D006801:Humans; D008297:Male; D008807:Mice, Inbred BALB C; D009570:Nitriles / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use; D017382:Reactive Oxygen Species / Q000378:metabolism*; D014947:Wounds and Injuries / Q000150:complications / Q000188:drug therapy / Q000382:microbiology
D000900:Anti-Bacterial Agents; D009570:Nitriles; D017382:Reactive Oxygen Species; C011206:cyanogen; D006046:Gold; D006108:Graphite
Biologically relevant levels; Gold nanoparticles; Graphitic carbon nitride; Reactive oxygen species; Wound disinfection