Label/quencher-free detection of single-nucleotide changes in DNA using isothermal amplification and G-quadruplexes.
We report an analytical method that exploits the interaction between G-quadruplexes and thioflavin T (ThT), for detecting mutant DNA species containing single-base changes. This system is a label/quencher-free fluorescence enhancement system based on rolling circle amplification (RCA)-responsive G-quadruplex formation, which enables a highly selective detection of T790M SNPs in the gene encoding the epidermal growth factor receptor, EGFR.
Department of Bioscience and Biotechnology, Konkuk University, Seoul 05029, Republic of Korea. kimde@konkuk.ac.kr.
Il Joon
IJ
Lee
Department of Bioscience and Biotechnology, Konkuk University, Seoul 05029, Republic of Korea. kimde@konkuk.ac.kr.
Nam-In
NI
Goo
Department of Bioscience and Biotechnology, Konkuk University, Seoul 05029, Republic of Korea. kimde@konkuk.ac.kr.
Dong-Eun
DE
Kim
D052160:Benzothiazoles; D015374:Biosensing Techniques; D004247:DNA / Q000032:analysis*; D066246:ErbB Receptors / Q000235:genetics; D054856:G-Quadruplexes; D006801:Humans; D021141:Nucleic Acid Amplification Techniques; D009711:Nucleotides; D020641:Polymorphism, Single Nucleotide; D013050:Spectrometry, Fluorescence; D013844:Thiazoles
D052160:Benzothiazoles; D009711:Nucleotides; D013844:Thiazoles; C009462:thioflavin T; D004247:DNA; C512478:EGFR protein, human; D066246:ErbB Receptors