Crowned Ionic Liquids for Biomolecular Interaction Analysis.
On the basis of affinity recognition with positively charged side chains in peptides and proteins, a series of crowned 1,2,3-triazolium ionic liquids (CIL 1-6) was developed and found to be capable of quantitatively extracting peptides and proteins from the aqueous layer into the ionic liquid phase. All of the synthesized CIL 1-6 are liquid at room temperature. This is the first example of biomolecular recognition of both lysine- and arginine-containing peptides and proteins by CILs in pure ionic liquid phase.
Department of Chemistry and Biochemistry, National Chung Cheng University , Minhsiung, Chiayi 62102, Taiwan, Republic of China.
Ming-Chung
MC
Tseng
Department of Chemistry and Biochemistry, National Chung Cheng University , Minhsiung, Chiayi 62102, Taiwan, Republic of China.
Tsu-Chun
TC
Yuan
Department of Chemistry and Biochemistry, National Chung Cheng University , Minhsiung, Chiayi 62102, Taiwan, Republic of China.
Zhuo
Z
Li
Department of Chemistry and Biochemistry, National Chung Cheng University , Minhsiung, Chiayi 62102, Taiwan, Republic of China.
Yen-Ho
YH
Chu
D052578:Ionic Liquids / Q000138:chemical synthesis / Q000737:chemistry*; D015394:Molecular Structure; D010455:Peptides / Q000032:analysis* / Q000737:chemistry*; D011506:Proteins / Q000032:analysis* / Q000737:chemistry*; D014230:Triazoles / Q000138:chemical synthesis / Q000737:chemistry*
D052578:Ionic Liquids; D010455:Peptides; D011506:Proteins; D014230:Triazoles