Second trimester amniotic fluid bisphenol A concentration is associated with decreased birth weight in term infants.
Bisphenol A (BPA) is an endocrine disrupting chemical with ubiquitous environmental exposure. Animal studies have demonstrated that in utero BPA exposure leads to increased adult body weight. Our aim was to characterize human fetal BPA exposure by measuring BPA concentration in second trimester amniotic fluid (AF) samples and to study its relationship with birth weight (BW) in full term infants. To achieve these goals, we developed a total BPA assay utilizing derivatization with pentafluorobenzyl followed by analysis with LC-ECAPCI-MS/MS with a limit of detection of 0.08ng/mL and limit of quantification (LOQ) of 0.25ng/mL. The mean BW of infants with AF BPA 0.40-2.0ng/mL was 241.8g less than infants with AF BPA less than the LOQ after controlling for covariates (p=0.049). No effect was seen outside this range indicating a non-monotonic effect. Our data suggest that low level BPA exposure in utero decreases BW and needs further study.
Division of Endocrinology and Diabetes, The Children's Hospital of Philadelphia, United States; Department of Pediatrics, Perelman School of Medicine at the University of Pennsylvania, Philadelphia, PA 19104, United States; Penn SRP Center and Center of Excellence in Environmental Toxicology, Perelman School of Medicine, University of Pennsylvania Philadelphia, PA 19104, United States. Electronic address: pinneys@email.chop.edu.
Sara E
SE
Pinney
Penn SRP Center and Center of Excellence in Environmental Toxicology, Perelman School of Medicine, University of Pennsylvania Philadelphia, PA 19104, United States; Department of Systems Pharmacology and Translational Therapeutics, Perelman School of Medicine, University of Pennsylvania Philadelphia, PA 19104, United States.
Clementina A
CA
Mesaros
Penn SRP Center and Center of Excellence in Environmental Toxicology, Perelman School of Medicine, University of Pennsylvania Philadelphia, PA 19104, United States; AJ Drexel Autism Institute, Drexel University, Philadelphia, PA 19104, United States.
Nathaniel W
NW
Snyder
Penn SRP Center and Center of Excellence in Environmental Toxicology, Perelman School of Medicine, University of Pennsylvania Philadelphia, PA 19104, United States; Department of Systems Pharmacology and Translational Therapeutics, Perelman School of Medicine, University of Pennsylvania Philadelphia, PA 19104, United States.
Christine M
CM
Busch
Department of Biostatistics and Epidemiology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, United States.
Rui
R
Xiao
Division of Endocrinology and Diabetes, The Children's Hospital of Philadelphia, United States.
Sara
S
Aijaz
Division of Endocrinology and Diabetes, The Children's Hospital of Philadelphia, United States.
Naila
N
Ijaz
Penn SRP Center and Center of Excellence in Environmental Toxicology, Perelman School of Medicine, University of Pennsylvania Philadelphia, PA 19104, United States; Department of Systems Pharmacology and Translational Therapeutics, Perelman School of Medicine, University of Pennsylvania Philadelphia, PA 19104, United States.
Ian A
IA
Blair
Canadensis, PA 18325, United States.
Jeanne M
JM
Manson
D000653:Amniotic Fluid / Q000737:chemistry*; D001559:Benzhydryl Compounds / Q000032:analysis*; D002853:Chromatography, Liquid; D052244:Endocrine Disruptors / Q000032:analysis*; D005260:Female; D006801:Humans; D007230:Infant, Low Birth Weight; D057230:Limit of Detection; D010636:Phenols / Q000032:analysis*; D011247:Pregnancy; D011262:Pregnancy Trimester, Second; D011297:Prenatal Exposure Delayed Effects / Q000209:etiology* / Q000503:physiopathology; D053719:Tandem Mass Spectrometry; D000098826:Bisphenol A Compounds
D001559:Benzhydryl Compounds; D052244:Endocrine Disruptors; D010636:Phenols; C006780:bisphenol A; D000098826:Bisphenol A Compounds
Bisphenol A; Developmental origins of health and disease; In utero exposure