Ultrasensitive paper based nucleic acid detection realized by three-dimensional DNA-AuNPs network amplification.
A novel three-dimensional DNA-AuNPs network structure amplification strategy was employed to design a lateral flow biosensor by introducing streptavidin coated gold nanoparticles (Au-SA) in this paper. They act as amplification probes which aggregate numerous gold nanoparticles (AuNPs) on test line by forming a three-dimensional DNA-AuNPs network structure in the presence of target. Sensitive detection of nucleic acid with point-of-care analysis is significant for infectious agent, early diagnosis and treatment of genetic diseases. The use of these particles in rapid ultrasensitive point of care (POC) lateral flow assays lead to a linear range from 0.1pM to 250nM with a limit of detection of 0.01 pM without polymerase chain reaction (PCR). The proposed method could increase the sensitivity by 4 orders of magnitudes than traditional sandwich assays labeled with AuNPs. Furthermore, the assay owns good reproducibility and stability, which will prove practical diagnostic applications.
Ministry-of-Education Key Laboratory for the Synthesis and Application of Organic Functional Molecules, College of Chemistry and Chemical Engineering, Hubei University, Wuhan 430062, China.
Ya
Y
Gao
Ministry-of-Education Key Laboratory for the Synthesis and Application of Organic Functional Molecules, College of Chemistry and Chemical Engineering, Hubei University, Wuhan 430062, China.
Xilei
X
Deng
Hubei Collaborative Innovation Center for Advanced Organic Chemical Materials, Hubei University, Wuhan 430062, China; Ministry-of-Education Key Laboratory for the Synthesis and Application of Organic Functional Molecules, College of Chemistry and Chemical Engineering, Hubei University, Wuhan 430062, China.
Wei
W
Wen
Hubei Collaborative Innovation Center for Advanced Organic Chemical Materials, Hubei University, Wuhan 430062, China; Ministry-of-Education Key Laboratory for the Synthesis and Application of Organic Functional Molecules, College of Chemistry and Chemical Engineering, Hubei University, Wuhan 430062, China.
Xiuhua
X
Zhang
Hubei Collaborative Innovation Center for Advanced Organic Chemical Materials, Hubei University, Wuhan 430062, China; Ministry-of-Education Key Laboratory for the Synthesis and Application of Organic Functional Molecules, College of Chemistry and Chemical Engineering, Hubei University, Wuhan 430062, China. Electronic address: wangsf@hubu.edu.cn.
Shengfu
S
Wang
D015374:Biosensing Techniques / Q000295:instrumentation*; D004277:DNA, Single-Stranded / Q000737:chemistry; D004279:DNA, Viral / Q000032:analysis / Q000097:blood*; D004867:Equipment Design; D006046:Gold / Q000737:chemistry*; D006509:Hepatitis B / Q000097:blood* / Q000821:virology; D006515:Hepatitis B virus / Q000302:isolation & purification*; D006801:Humans; D057230:Limit of Detection; D053768:Metal Nanoparticles / Q000737:chemistry*; D021141:Nucleic Acid Amplification Techniques / Q000295:instrumentation; D010209:Paper; D019095:Point-of-Care Systems; D011934:Reagent Strips / Q000032:analysis*; D015203:Reproducibility of Results; D019809:Streptavidin / Q000737:chemistry
D004277:DNA, Single-Stranded; D004279:DNA, Viral; D011934:Reagent Strips; D006046:Gold; D019809:Streptavidin
Gold nanoparticles; Lateral flow assay; Network amplification; Nucleic acid