Prednison provokes serum and vasoactive substances in a mice model of immune thrombocytopenia.
OBJECTIVE
The main objective of this study was to investigate the variations of β-endorphin (β-EP), vasoactive intestinal peptide (VIP), serotonin (5-HT) and norepinephrine (NE) of immune thrombocytopenia (ITP) mice as well as the regulatory mechanism of prednison.


METHODS
Sixty BALB/c mice were randomly divided into control group, model group and prednison intervention group. ITP mice model was duplicated by injecting with glycoprotein-antiplatelet serum (GP-APS) except in control group. After ITP disease model was successful established, prednison was used in prednison intervention group. The β-EP, VIP, 5-HT and NE contents of ITP mice were detected by enzyme linked immunosorbent assay (ELISA).


RESULTS
Compared with the values in control group, the detection values of VIP and 5-HT in model group declined, while the detection values of β-EP and NE increased. Compared with prednison intervention group, the detection values of VIP and 5-HT in model group increased, while the detection values of β-EP and NE showed no significant change.


CONCLUSIONS
In this study, the β-EP, VIP, 5-HT and NE contents in ITP mice injected with GP-APS were changed by prednison. It shows that prednison as the first-line therapy for ITP with effective hemostasis function is likely to increasing the contents of VIP and 5-HT. These results suggest the therapeutic value of prednison for the treatment of ITP.
Dongzhimen Hosp. Beijing University of Chinese Medicine. No. 5 Haiyuncang, Dongcheng District, Beijing, China.
Ling
L
Zhang
Dongzhimen Hosp. Beijing University of Chinese Medicine. No. 5 Haiyuncang, Dongcheng District, Beijing, China.
Ke
K
Chen
Dongzhimen Hosp. Beijing University of Chinese Medicine. No. 5 Haiyuncang, Dongcheng District, Beijing, China.
Tiantian
T
Li
School of Pharmacy, Xi'an Medical College, Shanxi 710021, China.
Hao
H
He
Dongzhimen Hosp. Beijing University of Chinese Medicine. No. 5 Haiyuncang, Dongcheng District, Beijing, China.
Li
L
Hou
No. 1 Hospital Affiliated to Guiyang College of TCM, GuiYang 550001, China.
Xiaoyong
X
Wu
School of Pharmacy, Xi'an Medical College, Shanxi 710021, China.
Yanping
Y
Sun
School of Pharmacy, Xi'an Medical College, Shanxi 710021, China.
Lei
L
Zheng
No. 1 Hospital Affiliated to Guangzhou University of Chinese Medicine, Guangzhou 510405, China.
Zhixiong
Z
Chen
School of Pharmacy, Xi'an Medical College, Shanxi 710021, China.
Bei
B
Qin
Dongzhimen Hosp. Beijing University of Chinese Medicine. No. 5 Haiyuncang, Dongcheng District, Beijing, China.
Xinyi
X
Chen
Dongzhimen Hosp. Beijing University of Chinese Medicine. No. 5 Haiyuncang, Dongcheng District, Beijing, China.
Shaodan
S
Tian
5-HT; Immune thrombocytopenia; Mice; Norepinephrine VIP; β-EP