Stathmin-like 4 is critical for the maintenance of neural progenitor cells in dorsal midbrain of zebrafish larvae.
A delicate balance between proliferating and differentiating signals is necessary to ensure proper growth and neuronal specification. By studying the developing zebrafish brain, we observed a specific and dynamic expression of a microtubule destabilizer gene, stathmin-like 4 (stmn4), in the dorsal midbrain region. The expression of stmn4 was mutually exclusive to a pan-neuronal marker, elavl3 that indicates its role in regulating neurogenesis. We showed the knockdown or overexpression of stmn4 resulted in premature neuronal differentiation in dorsal midbrain. We also generated stmn4 maternal-zygotic knockout zebrafish by the CRISPR/Cas9 system. Unexpectedly, only less than 10% of stmn4 mutants showed similar phenotypes observed in that of stmn4 morphants. It might be due to the complementation of the increased stmn1b expression observed in stmn4 mutants. In addition, time-lapse recordings revealed the changes in cellular proliferation and differentiation in stmn4 morphants. Stmn4 morphants displayed a longer G2phase that could be rescued by Cdc25a. Furthermore, the inhibition of Wnt could reduce stmn4 transcripts. These results suggest that the Wnt-mediated Stmn4 homeostasis is crucial for preventing dorsal midbrain from premature differentiation via the G2phase control during the neural keel stage.
Department of Life Science, National Taiwan University, Taipei, Taiwan.
Meng-Ju
MJ
Lin
Department of Life Science, National Taiwan University, Taipei, Taiwan.|Research Center for Developmental Biology and Regenerative Medicine, National Taiwan University, Taipei, Taiwan.|Center for Systems Biology, National Taiwan University, Taipei, Taiwan.|Center for Biotechnology, National Taiwan University, 1 Roosevelt Rd., Sec., 4, Taipei, Taiwan.
Shyh-Jye
SJ
Lee
D000818:Animals; D002454:Cell Differentiation; D008636:Mesencephalon / Q000166:cytology / Q000196:embryology*; D058953:Neural Stem Cells / Q000166:cytology / Q000378:metabolism*; D050777:Stathmin / Q000235:genetics / Q000378:metabolism*; D060449:Wnt Signaling Pathway; D015027:Zebrafish / Q000196:embryology* / Q000235:genetics; D029961:Zebrafish Proteins / Q000235:genetics / Q000378:metabolism*
D050777:Stathmin; D029961:Zebrafish Proteins