The role of gibberellins in improving the resistance of tebuconazole-coated maize seeds to chilling stress by microencapsulation.
Chilling stress during germination often causes severe injury. In the present study, maize seed germination and shoot growth under chilling stress were negatively correlated with the dose of tebuconazole in an exponential manner as predicted by the model Y = A + B × e(-x/k). Microencapsulation was an effective means of eliminating potential phytotoxic risk. The gibberellins (GAs) contents were higher after microencapsulation treatment than after conventional treatment when the dose of tebuconazole was higher than 0.12 g AI (active ingredient) kg-1seed. Further analysis indicated that microencapsulation can stimulate ent-kaurene oxidase (KO) activity to some extent, whereas GA 3-oxidase (GA3ox) and GA 2-oxidase (GA2ox) activities remained similar to those in the control. Genes encoding GA metabolic enzymes exhibited different expression patterns. Transcript levels of ZmKO1 increased in the microcapsule treatments compared to the control. Even when incorporated into microcapsules, tebuconazole led to the upregulation of ZmGA3ox1 at doses of less than 0.12 g AI kg-1seed and to the upregulation of ZmGA3ox2 when the dose was higher than 0.12 g AI kg-1seed. With increasing doses of microencapsulated tebuconazole, the transcript levels of ZmGA2ox4, ZmGA2ox5 and ZmGA2ox6 exhibited upward trends, whereas the transcript levels of ZmGA2ox7 exhibited a downward trend.
Key Laboratory of Integrated Pest Management in Crops, Ministry of Agriculture, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, 100193, China.
Lijuan
L
Yang
Key Laboratory of Integrated Pest Management in Crops, Ministry of Agriculture, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, 100193, China.
Daibin
D
Yang
Key Laboratory of Integrated Pest Management in Crops, Ministry of Agriculture, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, 100193, China.
Xiaojing
X
Yan
Key Laboratory of Integrated Pest Management in Crops, Ministry of Agriculture, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, 100193, China.
Li
L
Cui
Key Laboratory of Integrated Pest Management in Crops, Ministry of Agriculture, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, 100193, China.
Zhenying
Z
Wang
Key Laboratory of Integrated Pest Management in Crops, Ministry of Agriculture, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, 100193, China.
Huizhu
H
Yuan
D018533:Biomass; D053898:Biosynthetic Pathways / Q000187:drug effects / Q000235:genetics; D003080:Cold Temperature; D004339:Drug Compounding / Q000379:methods*; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D018525:Germination / Q000187:drug effects / Q000235:genetics; D005875:Gibberellins / Q000494:pharmacology*; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D018520:Plant Shoots / Q000187:drug effects / Q000201:enzymology / Q000254:growth & development; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012639:Seeds / Q000187:drug effects / Q000502:physiology*; D013312:Stress, Physiological / Q000187:drug effects* / Q000235:genetics; D014230:Triazoles / Q000494:pharmacology*; D003313:Zea mays / Q000187:drug effects / Q000235:genetics / Q000254:growth & development / Q000502:physiology*
D005875:Gibberellins; D010940:Plant Proteins; D012333:RNA, Messenger; D014230:Triazoles; C087114:tebuconazole