Whole-Genome Sequencing: Automated, Indexed Library Preparation.
This protocol describes an automated procedure for constructing an indexed Illumina DNA library. With this method, genomic DNA fragments are produced by sonication, using high-frequency acoustic energy to shear DNA. Double-stranded DNA (dsDNA) will fragment when exposed to the energy of adaptive focused acoustic shearing (AFA). The resulting DNA fragments are ligated to adaptors, amplified by polymer chain reaction (PCR), and subjected to size selection using magnetic beads. The product is suitable for use as template in whole-genome sequencing.
Elaine
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Mardis
W Richard
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McCombie
D057205:Automation, Laboratory / Q000379:methods*; D004247:DNA / Q000737:chemistry / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D011088:DNA Ligases / Q000378:metabolism; D015723:Gene Library; D023281:Genomics; D008967:Molecular Biology / Q000379:methods*; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D013010:Sonication
D004247:DNA; D011088:DNA Ligases