Daesiho-Tang Is an Effective Herbal Formulation in Attenuation of Obesity in Mice through Alteration of Gene Expression and Modulation of Intestinal Microbiota.
BACKGROUND
Obesity has become a major global health challenge due to its increasing prevalence, and the associated health risk. It is the main cause of various metabolic diseases including diabetes, hypertension, cardiovascular disease, stroke and certain forms of cancer.


RESULTS
In the present study we evaluated the anti-obesity property of Daesiho-tang (DSHT), an herbal medicine, using high fat diet (HFD)-induced obese mice as a model. Our results showed that DSHT ameliorated body weight gain, decreased total body fat, regulated expression of leptin and adiponectin genes of adipose tissue and exerted an anti-diabetic effect by attenuating fasting glucose level and serum insulin level in HFD-fed animals. In addition, DSHT-treatment significantly reduced total cholesterol (TC), triglycerides (TG) and increased high density lipoprotein-cholesterol (HDL), glutamic pyruvic transaminase (GPT) and glutamic oxaloacetic transaminase (GOT) levels in serum and reduced deposition of fat droplets in liver. DSHT treatment resulted in significantly increased relative abundance of bacteria including Bacteroidetes, Bacteroidetes/Firmicutes ratio, Akkermansia Bifidobacterium., Lactobacillus, and decreased the level of Firmicutes. Using RT2 profiler PCR array, 39 (46%) genes were found to be differentially expressed in HFD-fed mice compared to normal control. However, normal gene expressions were restored in 36 (92%) genes of HFD-fed mice, when co-exposed to DSHT.
The results of this study demonstrated that DSHT is an effective herbal formulation in attenuation of obesity in HFD-fed mice through alteration of gene expressions and modulation of intestinal microbiota.
Department of Rehabilitation Medicine of Korean Medicine, Dongguk University, Goyang, Republic of Korea.
Ahtesham
A
Hussain
Department of Otorhinolaryngology-Head and Neck Surgery, Korea University College of Medicine, Seoul, Republic of Korea.|Institute for Medical Device Clinical Trials, Korea University College of Medicine, Seoul, Republic of Korea.
Mukesh Kumar
MK
Yadav
Applied Surface Technology Inc., 11th Floor, Bldg. A, Advance Institutes of Convergence Technology, Yeongtong-gu, Suwon-si, Gyeonggi-do, 16229, Republic of Korea.
Shambhunath
S
Bose
Department of Rehabilitation Medicine of Korean Medicine, Dongguk University, Goyang, Republic of Korea.|Key Laboratory of Xin'an Medicine, Ministry of Education, Anhui University of Traditional Chinese Medicine, Meishan Road 103, Hefei, Anhui Province, People's Republic of China.
Jing-Hua
JH
Wang
Department of pathology, College of Korean Medicine, Dongguk University, Goyang, Republic of Korea.
Dongwoo
D
Lim
Department of Korean Rehabilitation Medicine, College of Korean Medicine, Gachon University, Incheon, Republic of Korea.
Yun-Kyung
YK
Song
Department of Preventive Medicine, College of Korean Medicine, Kyunghee University, Seoul, Republic of Korea.
Seong-Gyu
SG
Ko
Department of Rehabilitation Medicine of Korean Medicine, Dongguk University, Goyang, Republic of Korea.
Hojun
http://orcid.org/0000-0003-1038-0142
H
Kim
D052242:Adiponectin / Q000235:genetics; D000273:Adipose Tissue / Q000187:drug effects / Q000473:pathology; D000818:Animals; D001835:Body Weight / Q000187:drug effects; D002784:Cholesterol / Q000378:metabolism; D059305:Diet, High-Fat / Q000009:adverse effects; D004339:Drug Compounding; D000069196:Gastrointestinal Microbiome / Q000187:drug effects*; D005786:Gene Expression Regulation / Q000187:drug effects*; D005947:Glucose / Q000378:metabolism; D006706:Homeostasis / Q000187:drug effects; D007333:Insulin Resistance; D020738:Leptin / Q000235:genetics; D008099:Liver / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009765:Obesity / Q000188:drug therapy* / Q000378:metabolism / Q000382:microbiology* / Q000473:pathology; D009929:Organ Size / Q000187:drug effects; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use
D052242:Adiponectin; D020738:Leptin; D010936:Plant Extracts; D002784:Cholesterol; D005947:Glucose