Paeoniflorin ameliorates renal function in cyclophosphamide-induced mice via AMPK suppressed inflammation and apoptosis.
The study is to investigate the effects of paeoniflorin (PA) on renal function in cyclophosphamide-induced mice. Mice were injected with intraperitoneal cyclophosphamide (CYP, 200mg/kg) or saline respectively. Mice were treated with PA (15, 30mg/kg/day) or vehicle for the next 7 days. Then, mice were sacrificed to analyze the biochemical, histological parameters and mechanism research. Our results shown that PA significantly decreased the urine levels of uric acid and creatinine, serum and kidney levels of cytokines such as interleukin-6 (IL-6), interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α), PA also obviously attenuated the histological changes of the kidney tissues caused by CYP. Moreover, Western blot demonstrated that PA increased the AMPK levels and inhibited NF-κB signaling pathway and apoptosis in CYP-stimulated kidney tissues. In conclusion, PA might be considered as an effective agent in the amelioration of the kidney toxicity resulting from CYP treatment.
Department of Medical Imaging, The Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an, China.
Qiang
Q
Liu
Department of Neurology, The First Hospital of Xi'an, Xi'an, China.
Xuemei
X
Lin
Department of Gastroenterology, The Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an, China.
Hong
H
Li
Department of Gastroenterology, The Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an, China.
Jia
J
Yuan
Department of Medical Imaging, The Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an, China.
Yuping
Y
Peng
Department of Gastroenterology, The Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an, China. Electronic address: dong556@126.com.
Lei
L
Dong
Department of Gastroenterology, The Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an, China. Electronic address: daishejiao@163.com.
Shejiao
S
Dai
D055372:AMP-Activated Protein Kinases / Q000037:antagonists & inhibitors / Q000378:metabolism*; D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology* / Q000627:therapeutic use; D017209:Apoptosis / Q000187:drug effects / Q000502:physiology*; D003520:Cyclophosphamide / Q000633:toxicity*; D004305:Dose-Response Relationship, Drug; D005960:Glucosides / Q000494:pharmacology* / Q000627:therapeutic use; D007249:Inflammation / Q000188:drug therapy / Q000378:metabolism; D007668:Kidney / Q000187:drug effects / Q000473:pathology / Q000502:physiology*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D039821:Monoterpenes / Q000494:pharmacology* / Q000627:therapeutic use; D006027:Glycosides
D055372:AMP-Activated Protein Kinases; D000894:Anti-Inflammatory Agents, Non-Steroidal; D003520:Cyclophosphamide; D005960:Glucosides; D039821:Monoterpenes; C015423:peoniflorin; D006027:Glycosides
AMPK; Apoptosis; Cyclophosphamide; Inflammation; Paeoniflorin; Renal function