Physiological response and sulfur metabolism of the V. dahliae-infected tomato plants in tomato/potato onion companion cropping.
Companion cropping with potato onions (Allium cepa var. agrogatum Don.) can enhance the disease resistance of tomato plants (Solanum lycopersicum) to Verticillium dahliae infection by increasing the expressions of genes related to disease resistance. However, it is not clear how tomato plants physiologically respond to V. dahliae infection and what roles sulfur plays in the disease-resistance. Pot experiments were performed to examine changes in the physiology and sulfur metabolism of tomato roots infected by V. dahliae under the companion cropping (tomato/potato onion). The results showed that the companion cropping increased the content of total phenol, lignin and glutathione and increased the activities of peroxidase, polyphenol oxidase and phenylalanine ammonia lyase in the roots of tomato plants. RNA-seq analysis showed that the expressions of genes involved in sulfur uptake and assimilation, and the formation of sulfur-containing defense compounds (SDCs) were up-regulated in the V. dahlia-infected tomatoes in the companion cropping. In addition, the interactions among tomato, potato onion and V. dahliae induced the expression of the high- affinity sulfate transporter gene in the tomato roots. These results suggest that sulfur may play important roles in tomato disease resistance against V. dahliae.
Department of Horticulture, Northeast Agricultural University, No. 59 Mucai Street, XiangFang District, Harbin, Heilongjiang Province, 150030 China.|Department of Life Science and Agroforestry, Qiqihar University, No. 42 Wenhua Street, JianHua District, Qiqihar, Heilongjiang Province, 161006 China.
Xuepeng
X
Fu
Department of Horticulture, Northeast Agricultural University, No. 59 Mucai Street, XiangFang District, Harbin, Heilongjiang Province, 150030 China.
Chunxia
C
Li
Department of Horticulture, Northeast Agricultural University, No. 59 Mucai Street, XiangFang District, Harbin, Heilongjiang Province, 150030 China.
Xingang
X
Zhou
Department of Horticulture, Northeast Agricultural University, No. 59 Mucai Street, XiangFang District, Harbin, Heilongjiang Province, 150030 China.
Shouwei
S
Liu
Department of Horticulture, Northeast Agricultural University, No. 59 Mucai Street, XiangFang District, Harbin, Heilongjiang Province, 150030 China.
Fengzhi
F
Wu
D000383:Agriculture; D027321:Anion Transport Proteins / Q000235:genetics / Q000378:metabolism; D004156:Catechol Oxidase / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D005978:Glutathione / Q000378:metabolism; D008031:Lignin / Q000378:metabolism; D018551:Solanum lycopersicum / Q000254:growth & development / Q000378:metabolism* / Q000382:microbiology; D008715:Methionine / Q000378:metabolism; D019697:Onions / Q000254:growth & development* / Q000378:metabolism; D009195:Peroxidase / Q000378:metabolism; D010636:Phenols / Q000378:metabolism; D010650:Phenylalanine Ammonia-Lyase / Q000378:metabolism; D010940:Plant Proteins / Q000378:metabolism; D018517:Plant Roots / Q000201:enzymology / Q000378:metabolism; D018749:RNA, Plant / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D017423:Sequence Analysis, RNA; D013455:Sulfur / Q000378:metabolism*; D013482:Superoxide Dismutase / Q000378:metabolism; D020172:Verticillium / Q000502:physiology*
D027321:Anion Transport Proteins; D010636:Phenols; D010940:Plant Proteins; D018749:RNA, Plant; D013455:Sulfur; D008031:Lignin; D008715:Methionine; D004156:Catechol Oxidase; D009195:Peroxidase; D013482:Superoxide Dismutase; D010650:Phenylalanine Ammonia-Lyase; D005978:Glutathione