Lactobacillus fermentumATCC 23271 DisplaysIn vitroInhibitory Activities againstCandidaspp.
Lactobacilli are involved in the microbial homeostasis in the female genital tract. Due to the high prevalence of many bacterial diseases of the female genital tract and the resistance of microorganisms to various antimicrobial agents, alternative means to control these infections are necessary. Thus, this study aimed to evaluate the probiotic properties of well-characterizedLactobacillusspecies, includingL. acidophilus(ATCC 4356),L. brevis(ATCC 367),L. delbrueckiissp.delbrueckii(ATCC 9645),L. fermentum(ATCC 23271),L. paracasei(ATCC 335),L. plantarum(ATCC 8014), andL. rhamnosus(ATCC 9595), againstCandida albicans(ATCC 18804),Neisseria gonorrhoeae(ATCC 9826), andStreptococcus agalactiae(ATCC 13813). The probiotic potential was investigated by using the following criteria: (i) adhesion to host epithelial cells and mucus, (ii) biofilm formation, (iii) co-aggregation with bacterial pathogens, (iv) inhibition of pathogen adhesion to mucus and HeLa cells, and (v) antimicrobial activity. Tested lactobacilli adhered to mucin, co-aggregated with all genital microorganisms, and displayed antimicrobial activity. With the exception ofL. acidophilusandL. paracasei, they adhered to HeLa cells. However, onlyL. fermentumproduced a moderate biofilm and a higher level of co-aggregation and mucin binding. The displacement assay demonstrated that allLactobacillusstrains inhibitC. albicansbinding to mucin (p< 0.001), likely due to the production of substances with antimicrobial activity. Clinical isolates belonging to the most commonCandidaspecies associated to vaginal candidiasis were inhibited byL. fermentum. Collectively, our data suggest thatL. fermentumATCC 23271 is a potential probiotic candidate, particularly to complement candidiasis treatment, since presented with the best probiotic profile in comparison with the other tested lactobacilli strains.
Centro de Ciências Biológicas e da Saúde, Universidade Federal do Maranhão São Luís, Brazil.
Monique S
MS
do Carmo
Centro de Ciências da Saúde, Universidade CEUMA São Luís, Brazil.
Francisca M F
FM
Noronha
Centro de Ciências Biológicas e da Saúde, Universidade Federal do Maranhão São Luís, Brazil.
Mariana O
MO
Arruda
Centro de Ciências da Saúde, Universidade CEUMA São Luís, Brazil.
Ênnio P da Silva
ÊP
Costa
Centro de Ciências da Saúde, Universidade CEUMA São Luís, Brazil.
Maria R Q
MR
Bomfim
Centro de Ciências da Saúde, Universidade CEUMA São Luís, Brazil.
Andrea S
AS
Monteiro
Centro de Ciências da Saúde, Universidade CEUMA São Luís, Brazil.
Thiago A F
TA
Ferro
Centro de Ciências da Saúde, Universidade CEUMASão Luís, Brazil; Vascular Biology and Inflammation Section, Cardiovascular Division, King's College LondonLondon, UK.
Elizabeth S
ES
Fernandes
Centro de Detección Biomolecular, Benemérita Universidad Autónoma de Puebla Puebla, Mexico.
Jorge A
JA
Girón
Centro de Ciências Biológicas e da Saúde, Universidade Federal do MaranhãoSão Luís, Brazil; Centro de Ciências da Saúde, Universidade CEUMASão Luís, Brazil.
Valério
V
Monteiro-Neto
Candida; Lactobacillus fermentum; STD; genital infections; probiotic