miR-3117 regulates hepatocellular carcinoma cell proliferation by targeting PHLPPL.
Altered microRNA expression is associated with tumor proliferation, metastasis, and tumorigenesis. In this study, we studied the role of miR-3117 in hepatocellular carcinoma (HCC) cell proliferation and found that miR-3117 was upregulated in HCC tissues and cells. MTT assay, soft agar growth assay, BrdU assay, and cell cycle assay revealed that miR-3117 overexpression promoted HCC HepG2 cell proliferation and that knockdown of miR-3117 suppressed HepG2 proliferation. Mechanism analysis suggested PH domain and leucine-rich repeat protein phosphatase-like (PHLPPL) as the target of miR-3117. Luciferase reporter assay suggested that miR-3117 directly binds to the 3'UTR of PHLPPL. Double knockdown of miR-3117 and PHLPPL copied the phenotypes caused by miR-3117 overexpression, suggesting that miR-3117 contributes to the proliferation of HepG2 by targeting PHLPPL. Our study provided a target for HCC therapy.
Department of Hepatopathy, Liaocheng People's Hospital, 67 Dongchang West Road, Liaocheng, 252000, China.
Xia
X
Cui
Department of Hepatopathy, Liaocheng People's Hospital, 67 Dongchang West Road, Liaocheng, 252000, China.
Qingyan
Q
Li
Department of Hepatopathy, Liaocheng People's Hospital, 67 Dongchang West Road, Liaocheng, 252000, China. Heyukai94210899@163.com.
Yukai
Y
He
D006528:Carcinoma, Hepatocellular / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D049109:Cell Proliferation; D056945:Hep G2 Cells; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D009363:Neoplasm Proteins / Q000235:genetics / Q000378:metabolism*; D010749:Phosphoprotein Phosphatases / Q000235:genetics / Q000378:metabolism*; D012334:RNA, Neoplasm / Q000235:genetics / Q000378:metabolism*
D035683:MicroRNAs; D009363:Neoplasm Proteins; D012334:RNA, Neoplasm; C541572:PHLPP2 protein, human; D010749:Phosphoprotein Phosphatases
Hepatocellular carcinoma; PHLPPL; miR-3117; microRNA