Detection of Hemoglobin New York [β113 (G15) Val→Glu, GTG>GAG] in a Thai Woman by Capillary Electrophoresis.
Hemoglobin (Hb) New York [β113 (G15) Val→Glu, GTG>GAG] is a very rare β-chain variant found in Thailand. This variant is often missed by routine laboratory testing because Hb New York and Hb A have the identical retention time on high performance liquid chromatography. We reported here for the first time that the detection of Hb New York in a Thai woman by using capillary electrophoresis (CE). A peak of Hb New York located ahead of Hb A at the electrophoretic zone 11 with a level of 42.8 %. The DNA sequencing revealed the GTG>GAG mutation at codon 113 for Hb New York on one allele of β-globin gene. Therefore, the CE has a high efficiency to prevent the misinterpretation of hemoglobin analysis in patients who are heterozygote of this variant.
School of Allied Health Sciences, University of Phayao, Phayao, Thailand.
Sitthichai
S
Panyasai
Department of Medical Technology, Faculty of Associated Medical Sciences, Chiang-Mai University, 110 Intawaroros Road, Chiang Mai, 50200 Thailand.
Sakorn
S
Pornprasert
Capillary electrophoresis; Detection; Hb New York; High performance liquid chromatography; Thai woman