Non-Enzymatic Oligomerization of 3', 5' Cyclic AMP.
Recent studies illustrate that short oligonucleotide sequences can be easily produced from nucleotide precursors in a template-free non-enzymatic way under dehydrating conditions, i.e. using essentially dry materials. Here we report that 3',5' cyclic AMP may also serve as a substrate of the reaction, which proceeds under moderate conditions yet with a lower efficiency than the previously reported oligomerization of 3',5' cyclic GMP. Optimally the oligomerization requires (i) a temperature of 80°C, (ii) a neutral to alkaline environment and (iii) a time on the order of weeks. Differences in the yield and required reaction conditions of the oligomerizations utilizing 3',5' cGMP and cAMP are discussed in terms of the crystal structures of the compounds. Polymerization of 3',5' cyclic nucleotides, whose paramount relevance in a prebiotic chemistry context has been widely accepted for decades, supports the possibility that the origin of extant genetic materials might have followed a direct uninterrupted path since its very beginning, starting from non-elaborately pre-activated monomer compounds and simple reactions.
Istituto di Biologia e Patologia Molecolari, CNR, Piazzale Aldo Moro, 5, Rome, 00185, Italy.
Giovanna
G
Costanzo
Dipartimento di Biologia e Biotecnologie "Charles Darwin", "Sapienza" Università di Roma, Piazzale Aldo Moro, 5, Rome, 00185, Italy.
Samanta
S
Pino
Department of Ecology and Biology, "La Tuscia" University, Viterbo, 01100, Italy.
Anna Maria
AM
Timperio
Institute of Biophysics, Academy of Sciences of the Czech Republic, Královopolská 135, CZ-61265, Brno, Czech Republic.|CEITEC-Central European Institute of Technology, Masaryk University, Kamenice 5, CZ-62500, Brno, Czech Republic.
Judit E
JE
Šponer
Institute of Biophysics, Academy of Sciences of the Czech Republic, Královopolská 135, CZ-61265, Brno, Czech Republic.|CEITEC-Central European Institute of Technology, Masaryk University, Kamenice 5, CZ-62500, Brno, Czech Republic.
Jiří
J
Šponer
Institute of Biophysics, Academy of Sciences of the Czech Republic, Královopolská 135, CZ-61265, Brno, Czech Republic.
Olga
O
Nováková
CEITEC-Central European Institute of Technology, Masaryk University, Kamenice 5, CZ-62500, Brno, Czech Republic.
Ondrej
O
Šedo
CEITEC-Central European Institute of Technology, Masaryk University, Kamenice 5, CZ-62500, Brno, Czech Republic.
Zbyněk
Z
Zdráhal
Istituto Pasteur Italia-Fondazione Cenci-Bolognetti, c/o Dipartimento di Biologia e Biotecnologie "Charles Darwin", "Sapienza" Università di Roma, P.le Aldo Moro, 5, Rome, 00185, Italy.
Ernesto
E
Di Mauro
D006152:Cyclic GMP / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D058105:Polymerization
D006152:Cyclic GMP