Reprogramming Enhancers in Somatic Cell Nuclear Transfer, iPSC Technology, and Direct Conversion.
Direct conversion of cell fate is a core technology for future regenerative medicine. However, the low reprogramming efficiency and low reproducibility are the largest obstacles blocking translational research of direct conversion technology. This review lists the major reprogramming enhancers verified in the representative reprogramming technologies of somatic cell nuclear transfer, induced pluripotent stem cell technology, and direct conversion, and categorizes them according to their functions. Ultimately, we strive to provide a practical plan to overcome the low reprogramming efficiency and low reproducibility of direct conversion.
Stem Cells and Regenerative Bioengineering Institute in Kangstem Biotech, Biomedical Science Building, #81 Seoul National University, Seoul, 08826, South Korea.
Daekee
D
Kwon
Stem Cells and Regenerative Bioengineering Institute in Kangstem Biotech, Biomedical Science Building, #81 Seoul National University, Seoul, 08826, South Korea.
Minjun
M
Ji
Stem Cells and Regenerative Bioengineering Institute in Kangstem Biotech, Biomedical Science Building, #81 Seoul National University, Seoul, 08826, South Korea.
Seunghee
S
Lee
Stem Cells and Regenerative Bioengineering Institute in Kangstem Biotech, Biomedical Science Building, #81 Seoul National University, Seoul, 08826, South Korea. kwseo@kangstem.com.|Research Institute for Veterinary Medicine, College of Veterinary Medicine, Seoul National University, Seoul, 08826, South Korea. kwseo@kangstem.com.
Kwang Won
KW
Seo
Stem Cells and Regenerative Bioengineering Institute in Kangstem Biotech, Biomedical Science Building, #81 Seoul National University, Seoul, 08826, South Korea. kangpub@snu.ac.kr.|Adult Stem Cell Research Center, College of Veterinary Medicine, Seoul National University, Seoul, 08826, South Korea. kangpub@snu.ac.kr.
Kyung-Sun
KS
Kang
D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods; D002454:Cell Differentiation; D065150:Cellular Reprogramming; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000166:cytology* / Q000378:metabolism; D053652:Nuclear Transfer Techniques; D044968:Regenerative Medicine / Q000379:methods / Q000639:trends; D015203:Reproducibility of Results
Direct conversion; Induced pluripotent stem cells; Reprogramming; Reprogramming efficiency; Reprogramming enhancer; Somatic cell nuclear transfer