Imaging technique for real-time temperature monitoring during cryotherapy of lesions.
Noninvasive real-time temperature imaging during thermal therapies is able to significantly improve clinical outcomes. An optoacoustic (OA) temperature monitoring method is proposed for noninvasive real-time thermometry of vascularized tissue during cryotherapy. The universal temperature-dependent optoacoustic response (ThOR) of red blood cells (RBCs) is employed to convert reconstructed OA images to temperature maps. To obtain the temperature calibration curve for intensity-normalized OA images, we measured ThOR of 10 porcine blood samples in the range of temperatures from 40°C to ?16°C and analyzed the data for single measurement variations. The nonlinearity (?Tmax) and the temperature of zero OA response (T0) of the calibration curve were found equal to 11.4±0.1°C and ?13.8±0.1°C, respectively. The morphology of RBCs was examined before and after the data collection confirming cellular integrity and intracellular compartmentalization of hemoglobin. For temperatures below 0°C, which are of particular interest for cryotherapy, the accuracy of a single temperature measurement was ±1°C, which is consistent with the clinical requirements. Validation of the proposed OA temperature imaging technique was performed for slow and fast cooling of blood samples embedded in tissue-mimicking phantoms.
Tomowave Laboratories, Inc., 6550 Mapleridge Street, Suite 124, Houston, Texas 77081-4629, United States.
Elena
E
Petrova
Tomowave Laboratories, Inc., 6550 Mapleridge Street, Suite 124, Houston, Texas 77081-4629, United StatesbPinami, LLC, 10718 Silkwood Drive, Houston, Texas 77031, United States.
Anton
A
Liopo
Tomowave Laboratories, Inc., 6550 Mapleridge Street, Suite 124, Houston, Texas 77081-4629, United States.
Vyacheslav
V
Nadvoretskiy
Tomowave Laboratories, Inc., 6550 Mapleridge Street, Suite 124, Houston, Texas 77081-4629, United StatescPhotoSound Technologies, Inc., 5658 Sylmar Street, Houston, Texas 77081, United States.
Sergey
S
Ermilov
D000818:Animals; D017679:Cryotherapy / Q000379:methods*; D004912:Erythrocytes / Q000502:physiology / Q000528:radiation effects; D005260:Female; D007091:Image Processing, Computer-Assisted; D008297:Male; D008954:Models, Biological; D019047:Phantoms, Imaging; D061088:Photoacoustic Techniques / Q000379:methods*; D015203:Reproducibility of Results; D013552:Swine; D013696:Temperature; D062125:Thermometry / Q000379:methods*