Tracking E. coli runs and tumbles with scattering solutions and digital holographic microscopy.
We use in-line digital holographic microscopy to image freely swimming E. coli. We show that fitting a light scattering model to E. coli holograms can yield quantitative information about the bacterium's body rotation and tumbles, offering a precise way to track fine details of bacterial motility. We are able to extract the cell's three-dimensional (3D) position and orientation and recover behavior such as body angle rotation during runs, tumbles, and pole reversal. Our technique is label-free and capable of frame rates limited only by the camera.
Anna
A
Wang
Rees F
RF
Garmann
Vinothan N
VN
Manoharan
D004867:Equipment Design; D004926:Escherichia coli; D006696:Holography / Q000379:methods*; D008853:Microscopy / Q000379:methods*; D012399:Rotation