Effects of FTY720 (Fingolimod) on Proliferation, Differentiation, and Migration of Brain-Derived Neural Stem Cells.
Insufficient proliferation, differentiation, and migration are the main pitfalls of neural stem cells (NSCs) in reparative therapeutics for the central nervous system (CNS) diseases. The potent lipid mediator sphingosine-1-phosphate (S1P) regulates cells' biological behavior broadly in the CNS. However, the effects of activating S1P on NSCs are not quite clear. In the current study, FTY720 (Fingolimod), an analog of S1P, was employed to induce the proliferation, differentiation, and migration of cultured brain-derived NSCs. The results indicated that proliferation and migration ability of NSCs were promoted by FTY720. Though we observed no obvious neuron prefers differentiation of NSCs, there were more protoplasmic astrocytes developed in the presence of certain concentration of FTY720. This work gives more comprehensive understanding of how FTY720 affects NSCs.
Department of Rehabilitation Medicine, The 2nd Affiliated Hospital of Chongqing Medical University, Chongqing 400010, China.
Botao
0000-0001-8513-3416
B
Tan
Department of Rehabilitation Medicine, The 2nd Affiliated Hospital of Chongqing Medical University, Chongqing 400010, China.
Zeruxin
Z
Luo
Department of Rehabilitation Medicine, The 2nd Affiliated Hospital of Chongqing Medical University, Chongqing 400010, China; Physical Examination Center, Chongqing General Hospital, Chongqing, China.
Yan
Y
Yue
The 3rd Department of Research Institute of Surgery, Daping Hospital, The Third Military Medical University, State Key Laboratory of Trauma, Burns and Combined Injury, Chongqing, China.
Yuan
Y
Liu
Department of Rehabilitation Medicine, The 2nd Affiliated Hospital of Chongqing Medical University, Chongqing 400010, China.
Li
L
Pan
Department of Rehabilitation Medicine, The 2nd Affiliated Hospital of Chongqing Medical University, Chongqing 400010, China; Kuanren Rehabilitation Hospital, The 2nd Affiliated Hospital of Chongqing Medical University, Chongqing, China.
Lehua
L
Yu
Department of Rehabilitation Medicine, The 2nd Affiliated Hospital of Chongqing Medical University, Chongqing 400010, China.
Ying
0000-0002-6623-5254
Y
Yin