Identification and genetic characterization of classical swine fever virus isolates in Brazil: a new subgenotype.
The classical swine fever (CSF) is a highly contagious viral disease of pigs and wild boar. The CSF causes great economic losses for pork production and the occurrence of the disease is notifiable to the OIE. The objective of this work was to identify and characterize CSF virus isolates from Brazil. Seven viral isolates were obtained and the full-length E2 sequences were analyzed. Phylogenetic analysis revealed a different segregation pattern between Brazilian isolates and members of subgenotype 1.1, forming a separate group within genotype 1. Genetic distance analysis suggested the existence of two new subgenotypes, designated subgenotypes 1.5 and 1.6.
Department of Veterinary Medicine, Federal Rural University of Pernambuco, Rua Dom Manoel s/n, Dois Irmãos, Recife, Pernambuco, 52171-900, Brazil.|Laboratório Nacional Agropecuário (LANAGRO/PE), Recife, Pernambuco, 52171-030, Brazil.
Marta N F
MN
Silva
Department of Veterinary Medicine, Federal Rural University of Pernambuco, Rua Dom Manoel s/n, Dois Irmãos, Recife, Pernambuco, 52171-900, Brazil.
Diogo M F
DM
Silva
Laboratório Nacional Agropecuário (LANAGRO/PE), Recife, Pernambuco, 52171-030, Brazil.
Adriana S
AS
Leite
Department of Veterinary Medicine, Federal Rural University of Pernambuco, Rua Dom Manoel s/n, Dois Irmãos, Recife, Pernambuco, 52171-900, Brazil.|Federal University of Pernambuco, Vitória de Santo Antão, Recife, Pernambuco, 55608-680, Brazil.
Ana L V
AL
Gomes
Department of Genetics, Federal University of Pernambuco, Recife, Pernambuco, 50670-901, Brazil.
Antonio C
AC
Freitas
Department of Veterinary Medicine, Federal Rural University of Pernambuco, Rua Dom Manoel s/n, Dois Irmãos, Recife, Pernambuco, 52171-900, Brazil.
Jose W
JW
Pinheiro-Junior
Department of Veterinary Medicine, Federal Rural University of Pernambuco, Rua Dom Manoel s/n, Dois Irmãos, Recife, Pernambuco, 52171-900, Brazil.
Roberto S
RS
Castro
Department of Veterinary Medicine, Federal Rural University of Pernambuco, Rua Dom Manoel s/n, Dois Irmãos, Recife, Pernambuco, 52171-900, Brazil. andreluizsjesus@gmail.com.|Department of Genetics, Federal University of Pernambuco, Recife, Pernambuco, 50670-901, Brazil. andreluizsjesus@gmail.com.
Andre L S
AL
Jesus
D000818:Animals; D000829:Animals, Domestic / Q000821:virology; D001938:Brazil; D006691:Classical Swine Fever / Q000821:virology*; D006692:Classical Swine Fever Virus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D005838:Genotype; D010802:Phylogeny; D034421:Sus scrofa / Q000821:virology; D013552:Swine; D014764:Viral Proteins / Q000235:genetics
D014764:Viral Proteins
Classical swine fever; E2 gene; phylogenetic analysis