Micronuclei and other nuclear abnormalities on Caiman latirostris (Broad-snouted caiman) hatchlings after embryonic exposure to different pesticide formulations.
This study aimed to evaluate the embryotoxicity and genotoxicity of pesticide commercial formulations widely used in soybean crops through the Micronucleus (MN) test and other Nuclear Abnormalities (NAs) in erythrocytes of broad-snouted caiman (Caiman latirostris), exposed by topical application through the eggshell. Embryos were exposed (during all incubation: 70 days approximately) to sub-lethal concentrations of two glyphosate formulations PanzerGold® (PANZ) and Roundup® Full II (RU) (500, 750, 1000µg/egg); to the endosulfan (END) formulation Galgofan® and the cypermethrin (CYP) formulation Atanor® (1, 10, 100, and 1000µg/ egg). Blood samples were taken at the moment of hatching from the spinal vein for the application of the MN test and analysis of other NAs in erythrocytes, as markers of genotoxicity. Results indicated a significant increase in the frequency of MN for PANZ1000, END 10, CYP 1 and CYP 100 (p<0.05), and in the frequency of other NAs including Buds: END 100, 1000 and CYP 10 (p<0.05), eccentric nuclei: END 1, 10, 1000, CYP 10, 100, 1000 (p<0.01) and END 100 (p<0.05), notched nuclei: END 1, 10 (p<0.01) and END 1000, CYP 10, 100,1000 (p<0.05), and total nuclear abnormalities: END 1, 10, 100, 1000, CYP 10, 100 and 1000 (p<0.01), and the positive control (PC) (p<0.05), compared with the negative control. It was demonstrated a concentration dependent-effect in MN frequency only for PANZ (R2=0.98; p<0.01). Our study demonstrated that commercial formulations of pesticides induced genotoxic effects on C. latirostris, and NAs are a good indicator of genotoxicity in this specie.
"Proyecto Yacaré'' - Lab. Zoología Aplicada: Anexo Vertebrados (FHUC-UNL/MMA), Av. Aristóbulo del Valle 8700, 3000 Santa Fe, Argetina; CONICET, Av Rivadavia 1917, C1033AAJ CABA, Argentina. Electronic address: evelynclg@hotmail.com.
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López González
"Proyecto Yacaré'' - Lab. Zoología Aplicada: Anexo Vertebrados (FHUC-UNL/MMA), Av. Aristóbulo del Valle 8700, 3000 Santa Fe, Argetina.
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Larriera
"Proyecto Yacaré'' - Lab. Zoología Aplicada: Anexo Vertebrados (FHUC-UNL/MMA), Av. Aristóbulo del Valle 8700, 3000 Santa Fe, Argetina; CONICET, Av Rivadavia 1917, C1033AAJ CABA, Argentina; Instituto de Ciencias Veterinarias del Litoral (ICiVet-CONICET), Esperanza, Santa Fe, Argentina.
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Siroski
"Proyecto Yacaré'' - Lab. Zoología Aplicada: Anexo Vertebrados (FHUC-UNL/MMA), Av. Aristóbulo del Valle 8700, 3000 Santa Fe, Argetina; CONICET, Av Rivadavia 1917, C1033AAJ CABA, Argentina; Cát. Toxicol. y Bioq. Legal, FBCB-UNL, Ciudad Universitaria, Paraje El Pozo S/N, 3000 Santa Fe, Argentina.
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Poletta
D000287:Administration, Topical; D000489:Alligators and Crocodiles / Q000378:metabolism*; D000818:Animals; D004249:DNA Damage; D004726:Endosulfan / Q000633:toxicity*; D004912:Erythrocytes / Q000187:drug effects; D005998:Glycine / Q000031:analogs & derivatives* / Q000633:toxicity; D006540:Herbicides / Q000633:toxicity*; D007306:Insecticides / Q000633:toxicity*; D015162:Micronucleus Tests; D009153:Mutagens / Q000633:toxicity; D010063:Ovum; D011722:Pyrethrins / Q000633:toxicity*; D013723:Teratogens / Q000633:toxicity; D014874:Water Pollutants, Chemical / Q000633:toxicity*; D000097797:Glyphosate
D004726:Endosulfan; D005998:Glycine; D006540:Herbicides; D007306:Insecticides; D009153:Mutagens; D011722:Pyrethrins; D013723:Teratogens; D014874:Water Pollutants, Chemical; D000097797:Glyphosate; C017160:cypermethrin
Cypermethrin; Embryo exposure; Endosulfan; Genotoxicity; Glyphosate; Reptiles