Ubiquitin-like protein FAT10 promotes bladder cancer progression by stabilizing survivin.
Human HLA-F adjacent transcript 10 (FAT10) is a member of the ubiquitin-like-modifier family of proteins, which have been implicated in cancer development. In addition, the Survivin protein promotes proliferation in bladder cancer (BC). In this study, we explored the link between FAT10 and Survivin. FAT10 expression was dramatically up-regulated in BC tissue samples, and Kaplan-Meier survival analysis revealed that BC patients with high FAT10 expression had shorter overall survival than those with low FAT10 expression. Moreover, RNAi-mediated FAT10 knockdown decreased Survivin protein levels and inhibited BC proliferation both in vitro and in vivo. FAT10 directly bound to and stabilized Survivin protein, thereby promoting cancer cell proliferation by inhibiting ubiquitin-mediated degradation. These results reveal a novel mechanism by which FAT10 promotes tumor proliferation by directly stabilizing Survivin protein in BC.
Department of General Surgery, Second Affiliated Hospital of Nanchang University, Nanchang, 33000, China.|Jiangxi Province Key Laboratory of Molecular Medicine, Nanchang, 33000, China.
Dingxiang
D
Dong
Department of Urology Surgery, Second Affiliated Hospital of Nanchang University, Nanchang, 33000, China.
Weifan
W
Jiang
Department of General Surgery, Second Affiliated Hospital of Nanchang University, Nanchang, 33000, China.
Jun
J
Lei
Department of General Surgery, Second Affiliated Hospital of Nanchang University, Nanchang, 33000, China.|Jiangxi Province Key Laboratory of Molecular Medicine, Nanchang, 33000, China.
Leifeng
L
Chen
Jiangxi Province Key Laboratory of Molecular Medicine, Nanchang, 33000, China.
Xiuxia
X
Liu
Jiangxi Province Key Laboratory of Molecular Medicine, Nanchang, 33000, China.
Jin
J
Ge
Department of General Surgery, Second Affiliated Hospital of Nanchang University, Nanchang, 33000, China.|Jiangxi Province Key Laboratory of Molecular Medicine, Nanchang, 33000, China.
Ben
B
Che
Department of Urology Surgery, Second Affiliated Hospital of Nanchang University, Nanchang, 33000, China.
Xiaoqing
X
Xi
Department of General Surgery, Second Affiliated Hospital of Nanchang University, Nanchang, 33000, China.|Jiangxi Province Key Laboratory of Molecular Medicine, Nanchang, 33000, China.
Jianghua
J
Shao
D000293:Adolescent; D000328:Adult; D000818:Animals; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D002648:Child; D002675:Child, Preschool; D018572:Disease-Free Survival; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D051034:Inhibitor of Apoptosis Proteins / Q000235:genetics / Q000378:metabolism*; D053208:Kaplan-Meier Estimate; D008297:Male; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D008875:Middle Aged; D055550:Protein Stability; D059748:Proteolysis; D034622:RNA Interference; D015398:Signal Transduction; D000077022:Survivin; D013997:Time Factors; D014162:Transfection; D047368:Tumor Burden; D054875:Ubiquitination; D014452:Ubiquitins / Q000235:genetics / Q000378:metabolism*; D001749:Urinary Bladder Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology / Q000628:therapy; D055815:Young Adult
C107489:BIRC5 protein, human; D051034:Inhibitor of Apoptosis Proteins; D000077022:Survivin; C109184:UBD protein, human; D014452:Ubiquitins
FAT10 protein; bladder carcinoma; stabilize; survivin protein; tumor promoter