How many papillomavirus species can go undetected in papilloma lesions?
A co-infection comprising to at least seven papillomavirus (PV) types was detected by next generation sequencing (NGS) of randomly primed rolling circle amplification (RCA) products of a bovine (Bos taurus) papilloma lesion from the Brazilian Amazon region. Six putative new PV types that could not be detected by commonly used PCR protocols were identified. Their overall L1 nucleotide identities were less than 90% compared to described PV species and types. L1 nucleotide BLAST sequence hits showed that each new type was related to Beta, Gamma, Dyokappa, Dyoeta, and Xipapillomavirus, as well as two likely new unclassified genera. Our results show that the employment of NGS is relevant to the detection and characterization of distantly related PV and is of major importance in co-infection studies. This knowledge will help us understand the biology and pathogenesis of PV, as well as contribute to disease control. Moreover, we can also conclude that there are many unknown circulating PVs.
Laboratório de Virologia, Faculdade de Veterinária, Universidade Federal do Rio Grande do Sul (Av. Bento Gonçalves, 9090, Prédio 42.602, CEP 91540-000, Porto Alegre, Brazil).
Cíntia
C
Daudt
Laboratório de Virologia, Faculdade de Veterinária, Universidade Federal do Rio Grande do Sul (Av. Bento Gonçalves, 9090, Prédio 42.602, CEP 91540-000, Porto Alegre, Brazil).|Centro de Ciências Biológicas e da Natureza, Universidade Federal do Acre (Campus Universitário, BR 364, Km 04 - Distrito Industrial- CEP: 69920-900, Rio Branco, Brazil).
Flavio R C
FRC
da Silva
Laboratório de Virologia, Faculdade de Veterinária, Universidade Federal do Rio Grande do Sul (Av. Bento Gonçalves, 9090, Prédio 42.602, CEP 91540-000, Porto Alegre, Brazil).|Universidade de Caxias do Sul (Rua Francisco Getúlio Vargas, 1130, 95070-560, Caxias do Sul, Brazil).
André F
AF
Streck
Laboratório de Virologia, Faculdade de Veterinária, Universidade Federal do Rio Grande do Sul (Av. Bento Gonçalves, 9090, Prédio 42.602, CEP 91540-000, Porto Alegre, Brazil).
Matheus N
MN
Weber
Laboratório de Biologia Molecular, Instituto de Pesquisas Veterinárias Desidério Finamor (IPVDF), Fundação Estadual de Pesquisa Agropecuária (Estrada do Conde, 6000, CEP 92990-000, Eldorado do Sul, Rio Grande do Sul, Brazil).
Fabiana Q
FQ
Mayer
Laboratório de Virologia, Faculdade de Veterinária, Universidade Federal do Rio Grande do Sul (Av. Bento Gonçalves, 9090, Prédio 42.602, CEP 91540-000, Porto Alegre, Brazil).
Samuel P
SP
Cibulski
Laboratório de Virologia, Faculdade de Veterinária, Universidade Federal do Rio Grande do Sul (Av. Bento Gonçalves, 9090, Prédio 42.602, CEP 91540-000, Porto Alegre, Brazil).
Cláudio W
CW
Canal
D000818:Animals; D001483:Base Sequence; D002417:Cattle; D060085:Coinfection / Q000662:veterinary / Q000821:virology; D004279:DNA, Viral / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D016679:Genome, Viral; D059014:High-Throughput Nucleotide Sequencing; D021141:Nucleic Acid Amplification Techniques; D016366:Open Reading Frames / Q000235:genetics; D010212:Papilloma / Q000473:pathology / Q000662:veterinary / Q000821:virology*; D027383:Papillomaviridae / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA
D004279:DNA, Viral