Effects of Chinese Medicinal Compound Jinmaitong on the Expression of Nitrotyrosine andNerve Growth Factor in the Dorsal Root Ganglia of Diabetic Rats.
Objective To study the effects of Chinese medicinal compound Jinmaitong(JMT) on the expressions of nitrotyrosine (NT) and nerve growth factor (NGF) in dorsal root ganglia of diabetic rats. Methods Experimental rat diabetic models were established by the intraperitoneal injection of streptozotocin. Rat models were then randomly divided into four groups including normal control group (Con group),diabetes mellitus group (DM group),Jinmaitong group(JMT group)(treated with JMT similar to the fifteen-fold dose of adult recommended dosage),and taurine group(Tau group)(treated with Taurine similar to the fifteen-fold dose of adult recommended dosage),with 10 rats in each group. The Con and DM groups were treated with distilled water at a daily dose of 1 ml/100 g. All rats were given intragastric administration for 16 weeks and then killed. Body weight and blood glucose were detected before and at the 4th,8th,12th,and 16th week after treatment. The pain threshold to mechanical stimulation with von Frey filament were carried out before death. The expressions of NT and NGF in dorsal root ganglion were detected by immunohistochemistry and Western blot analysis,respectively. Results Immunohistochemistry showed that the average optical density (AOD) of NT expression in DM group were significantly higher than those in control group (P=0.000),and the AOD of NGF was significantly lower than the control group (P=0.006).The AOD of NT(P=0.000,P=0.000) in both treatment groups decreased significantly and the AOD of NGF(P=0.000, P=0.004)significantly increased compared with DM group. The AOD of NT in JMT group was significantly lower than Tau group (P=0.004). Western blot analysis showed that the protein level of NT in DM group was significantly higher than that in control group (P=0.000),and the protein level of NGF was significantly lower than that in control group (P=0.000). Compared with the DM group,the protein level of NT in both treatment groups significantly decreased (P=0.001,P=0.000),and the protein level of NGF increased significantly (P=0.000,P=0.001). Conclusion Traditional Chinese medicine JMT can obviously up-regulate the expressions of NGF and reduce the NT levels in dorsal root ganglia of diabetic rats.
Department of TCM,PUMC Hospital,CAMS and PUMC,Beijing 100730,China.
Ya-Nan
YN
Wu
Department of TCM,PUMC Hospital,CAMS and PUMC,Beijing 100730,China.
Xiao-Chun
XC
Liang
Department of TCM,PUMC Hospital,CAMS and PUMC,Beijing 100730,China.
Dan
D
Yang
Department of TCM,PUMC Hospital,CAMS and PUMC,Beijing 100730,China.
Ling
L
Qu
Department of TCM,PUMC Hospital,CAMS and PUMC,Beijing 100730,China.
Wei
W
Liu
Department of TCM,PUMC Hospital,CAMS and PUMC,Beijing 100730,China.
Yun-Zhou
YZ
Gao
D000818:Animals; D001786:Blood Glucose; D001835:Body Weight; D003921:Diabetes Mellitus, Experimental / Q000188:drug therapy / Q000473:pathology; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D005727:Ganglia, Spinal / Q000187:drug effects / Q000378:metabolism*; D007150:Immunohistochemistry; D020932:Nerve Growth Factor / Q000378:metabolism*; D017288:Pain Threshold; D011897:Random Allocation; D051381:Rats; D014443:Tyrosine / Q000031:analogs & derivatives* / Q000378:metabolism
D001786:Blood Glucose; D004365:Drugs, Chinese Herbal; C583496:jinmaitong; C002744:3-nitrotyrosine; D014443:Tyrosine; D020932:Nerve Growth Factor