Digital barcodes of suspension array using laser induced breakdown spectroscopy.
We show a coding method of suspension array based on the laser induced breakdown spectroscopy (LIBS), which promotes the barcodes from analog to digital. As the foundation of digital optical barcodes, nanocrystals encoded microspheres are prepared with self-assembly encapsulation method. We confirm that digital multiplexing of LIBS-based coding method becomes feasible since the microsphere can be coded with direct read-out data of wavelengths, and the method can avoid fluorescence signal crosstalk between barcodes and analyte tags, which lead to overall advantages in accuracy and stability to current fluorescent multicolor coding method. This demonstration increases the capability of multiplexed detection and accurate filtrating, expanding more extensive applications of suspension array in life science.
Shenzhen Key Laboratory for Minimal Invasive Medical Technologies, Institute of optical imaging and sensing, Graduate School at Shenzhen, Tsinghua University, Shenzhen 518055, China.|Department of Physics, Tsinghua University, Beijing 100084, China.
Qinghua
Q
He
Shenzhen Key Laboratory for Minimal Invasive Medical Technologies, Institute of optical imaging and sensing, Graduate School at Shenzhen, Tsinghua University, Shenzhen 518055, China.|Department of Physics, Tsinghua University, Beijing 100084, China.
Yixi
Y
Liu
Shenzhen Key Laboratory for Minimal Invasive Medical Technologies, Institute of optical imaging and sensing, Graduate School at Shenzhen, Tsinghua University, Shenzhen 518055, China.|Department of Physics, Tsinghua University, Beijing 100084, China.
Yonghong
Y
He
Shenzhen Key Laboratory for Minimal Invasive Medical Technologies, Institute of optical imaging and sensing, Graduate School at Shenzhen, Tsinghua University, Shenzhen 518055, China.|Department of Physics, Tsinghua University, Beijing 100084, China.
Liang
L
Zhu
Shenzhen Key Laboratory for Minimal Invasive Medical Technologies, Institute of optical imaging and sensing, Graduate School at Shenzhen, Tsinghua University, Shenzhen 518055, China.|Department of Physics, Tsinghua University, Beijing 100084, China.
Yilong
Y
Zhang
Shenzhen Key Laboratory for Minimal Invasive Medical Technologies, Institute of optical imaging and sensing, Graduate School at Shenzhen, Tsinghua University, Shenzhen 518055, China.|Department of Physics, Tsinghua University, Beijing 100084, China.
Zhiyuan
Z
Shen