Separation and identification of antidepressants by acrylate-based monolithic column capillary electrochromatography with UV detection.
This paper details a method for separation and identification of six antidepressant compounds using an acrylate-based porous monolithic capillary. Detection takes place using CEC coupled with UV or mass spectrometric detection. The CEC-UV method provides an effective and efficient method for the separation and identification of the analytes. Spiked urine samples were utilized to check method capability, and a liquid-liquid extraction procedure was developed to perform sample extraction. Detection limits ranged from 10 to 30 ng/mL.
Department of Chemistry, Florida International University, Miami, FL, USA.
Hanzhuo
H
Fu
Department of Chemistry, Florida International University, Miami, FL, USA. mccordb@fiu.edu.
Bruce R
BR
McCord
D000179:Acrylates / Q000737:chemistry; D000928:Antidepressive Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000652:urine*; D053801:Capillary Electrochromatography / Q000379:methods*; D006801:Humans; D057230:Limit of Detection; D016014:Linear Models; D059625:Liquid-Liquid Extraction; D013058:Mass Spectrometry; D015203:Reproducibility of Results; D013056:Spectrophotometry, Ultraviolet
D000179:Acrylates; D000928:Antidepressive Agents
Antidepressant; CEC; Extraction; MS; Monolithic column