GINS2 regulates matrix metallopeptidase 9 expression and cancer stem cell property in human triple negative Breast cancer.
GINS2, a subunit of GINS complex, is critical for the initiation of DNA replication and DNA replication fork progression. The expression of GINS2 is misregulated in many malignant tumors, such as leukemia, breast cancer and melanoma. However, the role of GINS in breast cancer remains poorly characterized. We investigate the possible effect and particular mechanism of GINS in breast cancer cells. We showed that expression of GINS2 is enriched in triple negative breast cancer (TNBC) cell lines. Furthermore, GINS2 knockdown decreased the growth, invasive ability and stem-like property of TNBC cells. Mechanistically, silencing of GINS2 in TNBC cells caused dramatic decrease of matrix metalloproteinase-9 (MMP9). Finally, the abundance of GINS2 correlated with the advance stages of tumor in human TNBC patients. Our studies provided insight into the molecular regulation of TNBC progression and invasion. More importantly, our data suggest that GINS2 could be an outstanding therapeutic target for inhibiting invasive TNBC growth and metastasis.
Department of Urology, Chinese PLA General Hospital, Beijing, China.
Liang
L
Peng
Department of Urology, Chinese PLA General Hospital, Beijing, China.
Zhigang
Z
Song
School of Dentistry, University of California Los Angeles, Los Angeles, CA, USA.
Demeng
D
Chen
Department of Urology, Chinese PLA General Hospital, Beijing, China.
Ruixia
R
Linghu
Department of Urology, Chinese PLA General Hospital, Beijing, China.
Yingzhe
Y
Wang
Department of Urology, Chinese PLA General Hospital, Beijing, China.
Xingyang
X
Zhang
Department of Urology, Chinese PLA General Hospital, Beijing, China.
Xiaoxue
X
Kou
Department of Urology, Chinese PLA General Hospital, Beijing, China. Electronic address: yangjunlan301@sina.cn.
Junlan
J
Yang
Department of Urology, Chinese PLA General Hospital, Beijing, China. Electronic address: jiaosc@vip.sina.com.
Shunchang
S
Jiao
D002453:Cell Cycle; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D002868:Chromosomal Proteins, Non-Histone / Q000378:metabolism*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D055785:Gene Knockdown Techniques; D006801:Humans; D020780:Matrix Metalloproteinase 9 / Q000235:genetics* / Q000378:metabolism; D009361:Neoplasm Invasiveness; D014411:Neoplastic Stem Cells / Q000378:metabolism / Q000473:pathology*; D034741:RNA, Small Interfering / Q000378:metabolism; D064726:Triple Negative Breast Neoplasms / Q000201:enzymology* / Q000235:genetics* / Q000473:pathology; D015854:Up-Regulation / Q000235:genetics
D002868:Chromosomal Proteins, Non-Histone; C556406:GINS2 protein, human; D034741:RNA, Small Interfering; C579270:MMP9 protein, human; D020780:Matrix Metalloproteinase 9
Cancer stem cells; GINS; GINS2; MMP9; Triple negative breast cancer