RNA-seq based transcriptomic analysis uncovers α-linolenic acid and jasmonic acid biosynthesis pathways respond to cold acclimation in Camellia japonica.
Camellia is a well-known ornamental flower native to Southeast of Asia, including regions such as Japan, Korea and South China. However, most species in the genus Camellia are cold sensitive. To elucidate the cold stress responses in camellia plants, we carried out deep transcriptome sequencing of 'Jiangxue', a cold-tolerant cultivar of Camellia japonica, and approximately 1,006 million clean reads were generated using Illumina sequencing technology. The assembly of the clean reads produced 367,620 transcripts, including 207,592 unigenes. Overall, 28,038 differentially expressed genes were identified during cold acclimation. Detailed elucidation of responses of transcription factors, protein kinases and plant hormone signalling-related genes described the interplay of signal that allowed the plant to fine-tune cold stress responses. On the basis of global gene regulation of unsaturated fatty acid biosynthesis- and jasmonic acid biosynthesis-related genes, unsaturated fatty acid biosynthesis and jasmonic acid biosynthesis pathways were deduced to be involved in the low temperature responses in C. japonica. These results were supported by the determination of the fatty acid composition and jasmonic acid content. Our results provide insights into the genetic and molecular basis of the responses to cold acclimation in camellia plants.
Forestry and Fruit Tree Research Institute, Wuhan Academy of Agricultural Science and Technology, Wuhan 430075, China.
Qingyuan
Q
Li
Forestry and Fruit Tree Research Institute, Wuhan Academy of Agricultural Science and Technology, Wuhan 430075, China.|College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan 430070, China.
Sheng
S
Lei
College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan 430070, China.
Kebing
K
Du
Forestry and Fruit Tree Research Institute, Wuhan Academy of Agricultural Science and Technology, Wuhan 430075, China.|College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan 430070, China.
Lizhi
L
Li
Forestry and Fruit Tree Research Institute, Wuhan Academy of Agricultural Science and Technology, Wuhan 430075, China.|College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan 430070, China.
Xufeng
X
Pang
Forestry and Fruit Tree Research Institute, Wuhan Academy of Agricultural Science and Technology, Wuhan 430075, China.
Zhanchang
Z
Wang
Forestry and Fruit Tree Research Institute, Wuhan Academy of Agricultural Science and Technology, Wuhan 430075, China.
Ming
M
Wei
Forestry and Fruit Tree Research Institute, Wuhan Academy of Agricultural Science and Technology, Wuhan 430075, China.
Shao
S
Fu
National Key Laboratory of Crop Genetic Improvement, Wuhan 430070, China.
Limin
L
Hu
Forestry and Fruit Tree Research Institute, Wuhan Academy of Agricultural Science and Technology, Wuhan 430075, China.
Lin
L
Xu
D028244:Camellia / Q000235:genetics / Q000378:metabolism*; D003080:Cold Temperature; D003517:Cyclopentanes / Q000378:metabolism*; D004553:Electric Conductivity; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D059014:High-Throughput Nucleotide Sequencing; D008315:Malondialdehyde / Q000032:analysis; D054883:Oxylipins / Q000378:metabolism*; D010937:Plant Growth Regulators / Q000378:metabolism; D018515:Plant Leaves / Q000235:genetics / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D011494:Protein Kinases / Q000235:genetics / Q000378:metabolism; D018749:RNA, Plant / Q000737:chemistry / Q000378:metabolism*; D017423:Sequence Analysis, RNA; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D059467:Transcriptome; D017962:alpha-Linolenic Acid / Q000096:biosynthesis*
D003517:Cyclopentanes; D054883:Oxylipins; D010937:Plant Growth Regulators; D010940:Plant Proteins; D018749:RNA, Plant; D014157:Transcription Factors; D017962:alpha-Linolenic Acid; D008315:Malondialdehyde; C011006:jasmonic acid; D011494:Protein Kinases