Adenosine receptors expression in cardiac fibroblasts of patients with left ventricular dysfunction due to valvular disease.
BACKGROUND
Adenosine restores tissue homeostasis through the interaction with its membrane receptors (AR) expressed on fibroblasts, endothelial cells, smooth muscle cells and leukocytes, but their modulation is still not fully understood.


OBJECTIVE
To evaluate whether changes in the transcriptomic profiling of adenosine receptors (AR) occur in cardiac fibroblasts (CF) of patients (pts) with LV dysfunction due to valvular disease (V). The secondary aim was to compare in the same pts the results obtained at cardiac level with those found in circulating leukocytes.


METHODS
Auricle fragments were excised from 13 pts during prosthetic implantation while blood samples were collected from pts (n = 9) and from healthy subjects (C, n = 7). In 7 pts cardiac biopsy and blood samples were taken simultaneously. A human CF atrial cell line (cc) was used as control.


RESULTS
AR higher levels of mRNA expression were observed with real-time PCR in Vpts compared to C, both at cardiac (overexpression A1R:98%, A2AR:63%, A2BR:87%, A3R:85%, CD39:92%, CD73:93%) and at peripheral level (A1R vs C: p = .0056; A2AR vs C: p = .0173; A2BR vs C: p = .0272; A3R vs C: p = .855; CD39 vs C: p = .0001; CD73 vs C: p = .0091).


CONCLUSIONS
All AR subtypes were overexpressed in CF of Vpts. The same trends in AR expression at cardiac level was assessed on circulating leukocytes, thus opening a new road to minimally invasive studies of the adenosinergic system in cardiac patients.
a CNR, Institute of Clinical Physiology , Pisa , Italy.
Silvia
S
Del Ry
a CNR, Institute of Clinical Physiology , Pisa , Italy.
Manuela
M
Cabiati
a CNR, Institute of Clinical Physiology , Pisa , Italy.|b Department of Medical Sciences, Surgery and Neurosciences , University of Siena , Siena , Italy.
Veronica
V
Della Latta
b Department of Medical Sciences, Surgery and Neurosciences , University of Siena , Siena , Italy.
Stefania
S
Zimbone
b Department of Medical Sciences, Surgery and Neurosciences , University of Siena , Siena , Italy.
Mariarita
M
Natale
b Department of Medical Sciences, Surgery and Neurosciences , University of Siena , Siena , Italy.
Pietro Enea
PE
Lazzerini
c Department of Heart, Vessels and Thorax , University Hospital of Siena , Siena , Italy.
Francesco
F
Diciolla
b Department of Medical Sciences, Surgery and Neurosciences , University of Siena , Siena , Italy.
Pier Leopoldo
PL
Capecchi
b Department of Medical Sciences, Surgery and Neurosciences , University of Siena , Siena , Italy.
Franco
F
Laghi-Pasini
a CNR, Institute of Clinical Physiology , Pisa , Italy.
Maria Aurora
MA
Morales
D005260:Female; D005347:Fibroblasts / Q000378:metabolism / Q000473:pathology; D005786:Gene Expression Regulation; D006349:Heart Valve Diseases / Q000097:blood* / Q000150:complications / Q000503:physiopathology; D006801:Humans; D008297:Male; D008875:Middle Aged; D005810:Multigene Family; D009206:Myocardium / Q000378:metabolism / Q000473:pathology; D043683:Receptors, Adenosine A2 / Q000096:biosynthesis / Q000235:genetics*; D059467:Transcriptome / Q000235:genetics; D018487:Ventricular Dysfunction, Left / Q000097:blood* / Q000150:complications / Q000503:physiopathology
D043683:Receptors, Adenosine A2
Adenosine receptors; Real-Time PCR; cardiac fibroblasts; heart failure; leukocytes; mRNA expression