Does time difference of the acetylcholinesterase (AChE) inhibition in different tissues exist? A case study of zebra fish (Danio rerio) exposed to cadmium chloride and deltamethrin.
In order to illustrate time difference in toxic effects of cadmium chloride (CdCl2) and deltamethrin (DM), AChE activities were measured in different tissues, liver, muscle, brain, and gill, of Zebra fish (Danio rerio) across different concentrations in this research. The average AChE activity decreased comparing to 0.0 TU with DM (82.81% in 0.1 TU, 56.14% in 1.0 TU and 44.68% in 2.0 TU) and with CdCl2(74.68% in 0.1 TU, 52.05% in 1.0 TU and 50.14% in 2.0 TU) showed an overall decrease with the increase of exposure concentrations. According to Self-Organizing Map (SOM), the AChE activities were characterized in relation with experimental conditions, showing an inverse relationship with exposure time. As the exposure time was longer, the AChE activities were correspondingly lower. The AChE inhibition showed time delay in sublethal treatments (0.1 TU) in different tissues: the AChE was first inhibited in brain by chemicals followed by gill, muscle and liver (brain > gill > muscle > liver). The AChE activity was almost inhibited synchronously in higher environmental stress (1.0 TU and 2.0 TU). As the AChE inhibition can induce abnormal of behavior movement, these results will be helpful to the mechanism of stepwise behavior responses according to the time difference in different tissues rather than the whole body AChE activity.
Institute of Environment and Ecology, Shandong Normal University, Ji'nan 250014, People's Republic of China.
Tingting
T
Zhang
Institute of Environment and Ecology, Shandong Normal University, Ji'nan 250014, People's Republic of China.
Meiyi
M
Yang
Institute of Environment and Ecology, Shandong Normal University, Ji'nan 250014, People's Republic of China.
Hongwei
H
Pan
Institute of Environment and Ecology, Shandong Normal University, Ji'nan 250014, People's Republic of China.
Shangge
S
Li
Institute of Environment and Ecology, Shandong Normal University, Ji'nan 250014, People's Republic of China.
Baigang
B
Ren
Institute of Environment and Ecology, Shandong Normal University, Ji'nan 250014, People's Republic of China. Electronic address: zmren@sdnu.edu.cn.
Zongming
Z
Ren
Institute of Environment and Ecology, Shandong Normal University, Ji'nan 250014, People's Republic of China.
Na
N
Xing
Institute of Environment and Ecology, Shandong Normal University, Ji'nan 250014, People's Republic of China.
Luhuizi
L
Qi
Institute of Environment and Ecology, Shandong Normal University, Ji'nan 250014, People's Republic of China.
Qing
Q
Ren
Institute of Environment and Ecology, Shandong Normal University, Ji'nan 250014, People's Republic of China.
Shiguo
S
Xu
Institute of Environment and Ecology, Shandong Normal University, Ji'nan 250014, People's Republic of China.
Jie
J
Song
Institute of Environment and Ecology, Shandong Normal University, Ji'nan 250014, People's Republic of China.
Jingchun
J
Ma
D000110:Acetylcholinesterase / Q000187:drug effects* / Q000378:metabolism*; D000818:Animals; D001921:Brain / Q000201:enzymology; D019256:Cadmium Chloride / Q000633:toxicity*; D002800:Cholinesterase Inhibitors / Q000633:toxicity*; D005880:Gills / Q000201:enzymology; D008099:Liver / Q000201:enzymology; D009132:Muscles / Q000201:enzymology; D009570:Nitriles / Q000633:toxicity*; D009928:Organ Specificity; D011722:Pyrethrins / Q000633:toxicity*; D013997:Time Factors; D015027:Zebrafish
D002800:Cholinesterase Inhibitors; D009570:Nitriles; D011722:Pyrethrins; C017180:decamethrin; D000110:Acetylcholinesterase; D019256:Cadmium Chloride
AChE activity; Brain; Cross correlation analysis; Self-organizing map; Stepwise behavior response; Time difference