Brassinolide Increases Potato Root GrowthIn Vitroin a Dose-Dependent Way and Alleviates Salinity Stress.
Brassinosteroids (BRs) are steroidal phytohormones that regulate various physiological processes, such as root development and stress tolerance. In the present study, we showed that brassinolide (BL) affects potato rootin vitrogrowth in a dose-dependent manner. Low BL concentrations (0.1 and 0.01μg/L) promoted root elongation and lateral root development, whereas high BL concentrations (1-100μg/L) inhibited root elongation. There was a significant (P< 0.05) positive correlation between root activity and BL concentrations within a range from 0.01 to 100μg/L, with the peak activity of 8.238 mg TTC·g-1FW·h-1at a BL concentration of 100μg/L. Furthermore, plants treated with 50μg/L BL showed enhanced salt stress tolerance throughin vitrogrowth. Under this scenario, BL treatment enhanced the proline content and antioxidant enzymes' (superoxide dismutase, peroxidase, and catalase) activity and reduced malondialdehyde content in potato shoots. Application of BL maintain K+and Na+homeostasis by improving tissue K+/Na+ratio. Therefore, we suggested that the effects of BL on root development from stem fragments explants as well as on primary root development are dose-dependent and that BL application alleviates salt stress on potato by improving root activity, root/shoot ratio, and antioxidative capacity in shoots and maintaining K+/Na+homeostasis in potato shoots and roots.
Hunan Provincial Key Laboratory of Phytohormones and Growth Development, College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha 410128, China; School of Life Science and Environmental Resource, Yichun University, Yichun 336000, China.
Yueqing
0000-0001-7593-2062
Y
Hu
Hunan Provincial Key Laboratory of Phytohormones and Growth Development, College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha 410128, China.
Shitou
S
Xia
Hunan Provincial Key Laboratory of Phytohormones and Growth Development, College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha 410128, China.
Yi
Y
Su
Hunan Provincial Key Laboratory of Phytohormones and Growth Development, College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha 410128, China.
Huiqun
H
Wang
Hunan Provincial Key Laboratory of Phytohormones and Growth Development, College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha 410128, China.
Weigui
W
Luo
Hunan Provincial Key Laboratory of Phytohormones and Growth Development, College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha 410128, China.
Shengying
S
Su
Hunan Provincial Key Laboratory of Phytohormones and Growth Development, College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha 410128, China.
Langtao
0000-0003-1786-1950
L
Xiao
D000975:Antioxidants / Q000737:chemistry; D018533:Biomass; D060406:Brassinosteroids / Q000737:chemistry*; D002374:Catalase / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D006861:Hydrogen Peroxide / Q000737:chemistry; D008315:Malondialdehyde / Q000737:chemistry; D010641:Phenotype; D018517:Plant Roots / Q000187:drug effects* / Q000254:growth & development*; D018547:Plant Stems / Q000378:metabolism; D011188:Potassium / Q000737:chemistry; D011392:Proline; D054712:Salinity; D055049:Salt Tolerance; D012964:Sodium / Q000737:chemistry; D012965:Sodium Chloride / Q000737:chemistry; D011198:Solanum tuberosum / Q000187:drug effects* / Q000254:growth & development; D013260:Steroids, Heterocyclic / Q000737:chemistry*; D013482:Superoxide Dismutase / Q000378:metabolism
D000975:Antioxidants; D060406:Brassinosteroids; D013260:Steroids, Heterocyclic; D012965:Sodium Chloride; D008315:Malondialdehyde; D011392:Proline; D012964:Sodium; D006861:Hydrogen Peroxide; D002374:Catalase; D013482:Superoxide Dismutase; D011188:Potassium; C023623:brassinolide