Detection of the Ubiquitinome in Cells Undergoing Oncogene-Induced Senescence.
Senescent cells exhibit dramatic changes in protein post-translational modifications. Here, we describe a method, stable isotope labeling with amino acids in cell culture (SILAC) coupled to liquid chromatography tandem mass spectrometry (LC-MS/MS), to identify changes in the ubiquitinome in cells that have undergone oncogene-induced senescence.
Gene Expression and Regulation Program, The Wistar Institute, Philadelphia, PA, 19104, USA.
Hengrui
H
Zhu
Gene Expression and Regulation Program, The Wistar Institute, Philadelphia, PA, 19104, USA.|Cell and Molecular Biology Graduate Group, Perelman School of Medicine of the University of Pennsylvania, Philadelphia, PA, 19104, USA.
Linh
L
Le
Molecular and Cellular Oncology Program and Proteomics Core, The Wistar Institute, Philadelphia, PA, 19104, USA.
Hsin-Yao
HY
Tang
Molecular and Cellular Oncology Program and Proteomics Core, The Wistar Institute, Philadelphia, PA, 19104, USA.
David W
DW
Speicher
Gene Expression and Regulation Program, The Wistar Institute, Room 312, 3601 Spruce Street, Philadelphia, PA, 19104, USA. rzhang@wistar.org.
Rugang
R
Zhang
D002460:Cell Line; D016922:Cellular Senescence / Q000235:genetics; D002846:Chromatography, Affinity; D002853:Chromatography, Liquid / Q000379:methods; D005347:Fibroblasts / Q000378:metabolism; D011905:Genes, ras; D006801:Humans; D007553:Isotope Labeling; D009857:Oncogenes / Q000235:genetics*; D011499:Protein Processing, Post-Translational; D020543:Proteome; D040901:Proteomics / Q000379:methods; D053719:Tandem Mass Spectrometry / Q000379:methods; D014452:Ubiquitins / Q000302:isolation & purification / Q000378:metabolism*
D020543:Proteome; D014452:Ubiquitins
Liquid chromatography tandem mass spectrometry (LC-MS/MS); Post-translational modification; Senescence; Stable isotope labeling with amino acids in cell culture (SILAC); Ubiquitin; Ubiquitin-proteosome pathway; Ubiquitinome