Determination of Cell Cycle Stage and Mitotic Exit Through the Quantification of the Protein Levels of Known Mitotic Regulators.
There are multiple processes that occur at certain points during the cell cycle and that affect later steps. Impairment of such processes could cause delays or even completely abolish cell cycle progression. Therefore, it is extremely helpful in order to determine the potential consequences that interfering on a cellular process imposes on cell cycle progression to be able to precisely characterize the cell cycle stage by using molecular markers. Here, we describe the analysis of the protein levels of known mitotic regulators as molecular markers to monitor the progression of cells through the cell cycle by western blot in synchronized yeast cell cultures.
Centro Andaluz de Biología Molecular y Medicina Regenerativa (CABIMER), Avda. Américo Vespucio, s/n, P.C.T. Cartuja 93, 41092, Sevilla, Spain. cristina.cepeda@cabimer.es.
Cristina
C
Cepeda-García
D018929:Cell Culture Techniques / Q000379:methods; D002453:Cell Cycle; D018797:Cell Cycle Proteins / Q000032:analysis*; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D015151:Immunoblotting / Q000379:methods; D008938:Mitosis; D012441:Saccharomyces cerevisiae / Q000166:cytology*; D029701:Saccharomyces cerevisiae Proteins / Q000032:analysis*
D018797:Cell Cycle Proteins; D029701:Saccharomyces cerevisiae Proteins
Budding index; Cell cycle; Clb2; Synchrony; Western blot