Genome analysis revealed novel genotypes and recombination of the human parechoviruses prevalent in children in Eastern China.
BACKGROUND
Human parechovirus (HPeV) is a genus of virus in the familyPicornaviridae, having two species A and B. HPeVs are common infectious agents, usually causing mild diarrhea and respiratory disease in young children.


RESULTS
Here, we collected and sequenced the near complete genome sequences of 17 novel HPeVs from children with diarrhea in eastern China, which showed significant nucleotide sequence divergence. Phylogenetic analysis based on the complete genomes of these HPeV strains revealed that they belonged to seven different genotypes (including three putative novel genotypes), suggesting that HPeVs showed genotype diversity in this area. Recombination analysis indicated that one of the HPeVs, belonging to HPeV-1 with strain name 146Chzj02, was a recombinant generated by inter-genotype recombination among three HPeV strains belonging to three different genotypes, respectively.


CONCLUSIONS
Our data revealed the property of the genotype diversity of HPeVs prevalent in children with diarrhea in eastern China, which will be helpful in the future study of the viral evolution of HPeVs and the identification and typing of HPeVs in the clinical laboratory.
Nanjing Lishui People's Hospital, Lishui, Jiangsu 211200 China.
Xiangyang
X
Zhao
Nanjing Lishui People's Hospital, Lishui, Jiangsu 211200 China.
Yongqiang
Y
Shi
Nanjing Lishui People's Hospital, Lishui, Jiangsu 211200 China.
Yu
Y
Xia
Genome sequence; Human parechovirus; Phylogenetic analysis; Recombination