Comparison of the size of membrane microparticles of different cellular origin by dynamic light scattering.
Size of membrane microparticles (MPs) from blood plasma and MPs produced in vitro by activated endothelial cells (ECs), monocytes, THP-1 monocytic cells, granulocytes, and platelets was evaluated by dynamic light scattering. MPs were sedimented from the culture media, cell supernatants, and plasma at 20 000 g for 30 min. Average diameters of all types of MPs ranged from 300 to 600 nm. Plasma MPs had the smallest size. Close sizes were registered for MPs from platelets and THP-1 cells. MPs from monocytes were larger, and MPs from granulocytes and ECs were the largest ones. The data obtained indicate that the size of membrane MPs depends on the type of their cell-producers.
Institute of General Pathology and Pathophysiology, Moscow, Russia.
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IB
Alchinova
Russian Cardiology Research and Production Complex, Russian Ministry of Health, Moscow, Russia.
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Khaspekova
Russian Cardiology Research and Production Complex, Russian Ministry of Health, Moscow, Russia.
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Golubeva
Russian Cardiology Research and Production Complex, Russian Ministry of Health, Moscow, Russia.
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ON
Shustova
Russian Cardiology Research and Production Complex, Russian Ministry of Health, Moscow, Russia.
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Antonova
Institute of General Pathology and Pathophysiology, Moscow, Russia.
M Yu
MY
Karganov
Russian Cardiology Research and Production Complex, Russian Ministry of Health, Moscow, Russia. avmazurov@list.ru.
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Mazurov
D000704:Analysis of Variance; D001792:Blood Platelets / Q000378:metabolism; D002460:Cell Line; D055252:Cell-Derived Microparticles / Q000378:metabolism; D003470:Culture Media; D000067493:Dynamic Light Scattering; D042783:Endothelial Cells / Q000378:metabolism; D006098:Granulocytes / Q000378:metabolism; D006801:Humans; D009000:Monocytes / Q000378:metabolism; D010316:Particle Size; D014471:Umbilical Veins / Q000166:cytology / Q000378:metabolism
D003470:Culture Media