Determination of dasatinib in the tablet dosage form by ultra high performance liquid chromatography, capillary zone electrophoresis, and sequential injection analysis.
Dasatinib is a novel oral prescription drug proposed for treating adult patients with chronic myeloid leukemia. Three analytical methods, namely ultra high performance liquid chromatography, capillary zone electrophoresis, and sequential injection analysis, were developed, validated, and compared for determination of the drug in the tablet dosage form. The total analysis time of optimized ultra high performance liquid chromatography and capillary zone electrophoresis methods was 2.0 and 2.2 min, respectively. Direct ultraviolet detection with detection wavelength of 322 nm was employed in both cases. The optimized sequential injection analysis method was based on spectrophotometric detection of dasatinib after a simple colorimetric reaction with folin ciocalteau reagent forming a blue-colored complex with an absorbance maximum at 745 nm. The total analysis time was 2.5 min. The ultra high performance liquid chromatography method provided the lowest detection and quantitation limits and the most precise and accurate results. All three newly developed methods were demonstrated to be specific, linear, sensitive, precise, and accurate, providing results satisfactorily meeting the requirements of the pharmaceutical industry, and can be employed for the routine determination of the active pharmaceutical ingredient in the tablet dosage form.
GlaxoSmithKline, Currabinny, Carrigaline, Co. Cork, Ireland.|Faculty of Science, Department of Analytical Chemistry, Charles University, Czech Republic.
Aroa Garcia
AG
Gonzalez
Faculty of Science, Department of Analytical Chemistry, Charles University, Czech Republic.
Lukáš
L
Taraba
Faculty of Science, Department of Analytical Chemistry, Charles University, Czech Republic.
Jakub
J
Hraníček
Faculty of Science, Department of Analytical Chemistry, Charles University, Czech Republic.
Petr
P
Kozlík
Faculty of Science, Department of Analytical Chemistry, Charles University, Czech Republic.
Pavel
P
Coufal
D002626:Chemistry, Pharmaceutical / Q000379:methods* / Q000592:standards; D002851:Chromatography, High Pressure Liquid; D000069439:Dasatinib / Q000032:analysis*; D019075:Electrophoresis, Capillary; D006801:Humans; D057230:Limit of Detection; D015203:Reproducibility of Results; D013053:Spectrophotometry; D013607:Tablets / Q000737:chemistry* / Q000592:standards
D013607:Tablets; D000069439:Dasatinib
Capillary electrophoresis; Dasatinib; liquid chromatography; sequential injection analysis; tablet dosage form