Evaluation of 3M Molecular Detection System and ANSR Pathogen Detection System for rapid detection of Salmonella from egg products.
Isothermal amplification assay is a novel simple detection technology that amplifies DNA with high speed, efficiency, and specificity under isothermal conditions. The objective of this study was to evaluate the effectiveness of the 3M Molecular Detection System (MDS) and ANSR Pathogen Detection System (PDS) for the detection of Salmonella in egg products as compared to the Food and Drug Administration's Bacteriological Analytical Manual (BAM) culture method and a modified culture method (3M MDS and ANSR PDS preferred method). Two Salmonella ser. Enteritidis (18579, PT4; CDC_2010K_1441, PT8), one Salmonella ser. Heidelberg (607310-1), and one Salmonella ser. Typhimurium (0723) isolates were used in this study. Seven wet egg products and 13 dry egg products were inoculated with these strains individually at 1 to 5 CFU/25 g. One set of test portions was prepared following FDA BAM procedures [with lactose broth (LB) as pre-enrichment broth]. Another set of test portions was prepared using buffered peptone water (BPW) as pre-enrichment broth, as instructed by the 2 detection systems. Results from 3M MDS and ANSR PDS were 100% in agreement with their BPW-based culture method results. When LB was used as pre-enrichment broth, the number of Salmonella positive test portions (80 tested), identified with the BAM, 3M MDS, and ANSR PDS, were 63, 61, and 60, respectively. In conclusion, both 3M MDS and ANSR PDS Salmonella assays were as effective as their BPW based culture methods and were equivalent to the BAM culture method for the detection of Salmonella in egg products. These sensitive isothermal assays can be used as rapid detection tools for Salmonella in egg products provided that BPW is used as pre-enrichment broth.
Center for Food Safety and Applied Nutrition, Food and Drug Administration, College Park, MD 20740.|College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, China.
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L
Hu
National Institute for Microbial Forensics and Food and Agricultural Biosecurity, Department of Entomology and Plant Pathology, Oklahoma State University, Stillwater 74074.
L M
LM
Ma
College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, China.
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S
Zheng
Western Regional Research Center, U.S. Department of Agriculture, Agricultural Research Service, Albany, CA 94710.
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X
He
Center for Food Safety and Applied Nutrition, Food and Drug Administration, College Park, MD 20740.
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H
Wang
Center for Food Safety and Applied Nutrition, Food and Drug Administration, College Park, MD 20740.
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EW
Brown
Center for Food Safety and Applied Nutrition, Food and Drug Administration, College Park, MD 20740.
T S
TS
Hammack
Center for Food Safety and Applied Nutrition, Food and Drug Administration, College Park, MD 20740.
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G
Zhang
D000818:Animals; D001431:Bacteriological Techniques / Q000379:methods*; D003470:Culture Media; D004269:DNA, Bacterial / Q000032:analysis; D004531:Eggs / Q000382:microbiology*; D005506:Food Contamination / Q000032:analysis; D005516:Food Microbiology / Q000379:methods*; D012475:Salmonella / Q000302:isolation & purification*
D003470:Culture Media; D004269:DNA, Bacterial
LAMP; Salmonella; egg product; isothermal amplification assay