Neovestitol, an isoflavonoid isolated from Brazilian red propolis, reduces acute and chronic inflammation: involvement of nitric oxide and IL-6.
Isoflavonoids have been largely studied due to their distinct biological activities identified thus far. Herein, we evaluated the activity of neovestitol, an isoflavonoid isolated from Brazilian red propolis, in acute and chronic inflammation. As for acute inflammation, we found that neovestitol reduced neutrophil migration, leukocyte rolling and adhesion, as well as expression of ICAM-1 in the mesenteric microcirculation during lipopolysaccharide-induced acute peritonitis. No changes were observed in the levels of TNF-α, CXCL1/KC and CXCL2/MIP-2 upon pretreatment with neovestitol. The administration of an inducible nitric oxide synthase (iNOS) inhibitor abolished the inhibitory effects of neovestitol in neutrophil migration and ICAM-1 expression. Nitrite levels increased upon treatment with neovestitol. No effects of neovestitol were observed on the chemotaxis of neutrophils in vitro. As for chronic inflammation, neovestitol also reduced the clinical score and joint damage in a collagen-induced arthritis model. There was no change in the frequency of IL-17-producing TCD4+ cells. In addition, pretreatment with neovestitol reduced the levels of IL-6. These results demonstrate a potential anti-inflammatory activity of neovestitol, which may be useful for therapeutic purposes and/or as a nutraceutical.
Department of Physiological Sciences, Piracicaba Dental School, University of Campinas, Piracicaba, 13414 903, Brazil.
Marcelo
M
Franchin
Department of Immunology, Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto, 14049-900, Brazil.
David F
DF
Colón
Department of Physiological Sciences, Piracicaba Dental School, University of Campinas, Piracicaba, 13414 903, Brazil.
Marcos G
MG
da Cunha
Department of Pharmacology, Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto, 14049-900, Brazil.
Fernanda V S
FV
Castanheira
Department of Pharmacology, Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto, 14049-900, Brazil.
André L L
AL
Saraiva
Dental Research Division, Guarulhos University, Guarulhos, São Paulo, CEP: 07023-070, Brazil.
Bruno
B
Bueno-Silva
Department of Agri-Food industry, Food and Nutrition, "Luiz de Queiroz" College of Agriculture, University of São Paulo, Piracicaba, 13418-900, Brazil.
Severino M
SM
Alencar
Department of Immunology, Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto, 14049-900, Brazil.
Thiago M
TM
Cunha
Department of Physiological Sciences, Piracicaba Dental School, University of Campinas, Piracicaba, 13414 903, Brazil.
Pedro L
PL
Rosalen
D000208:Acute Disease; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000737:chemistry / Q000494:pharmacology / Q000627:therapeutic use; D001169:Arthritis, Experimental / Q000209:etiology / Q000517:prevention & control*; D001938:Brazil; D015496:CD4-Positive T-Lymphocytes / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D002460:Cell Line; D002465:Cell Movement / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002908:Chronic Disease; D016207:Cytokines / Q000378:metabolism; D005419:Flavonoids / Q000737:chemistry / Q000494:pharmacology / Q000627:therapeutic use*; D006146:Guanidines / Q000494:pharmacology; D015850:Interleukin-6 / Q000378:metabolism*; D008070:Lipopolysaccharides / Q000633:toxicity; D008642:Mesenteric Veins / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D051379:Mice; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D009504:Neutrophils / Q000166:cytology / Q000187:drug effects / Q000276:immunology; D009569:Nitric Oxide / Q000378:metabolism*; D010538:Peritonitis / Q000209:etiology / Q000517:prevention & control*; D011429:Propolis / Q000737:chemistry* / Q000378:metabolism
D000893:Anti-Inflammatory Agents; D016207:Cytokines; D005419:Flavonoids; D006146:Guanidines; D015850:Interleukin-6; D008070:Lipopolysaccharides; C000627678:neovestitol; D009569:Nitric Oxide; D011429:Propolis; C004479:pimagedine