Urinary orosomucoid: validation of an automated immune turbidimetric test and its possible clinical use.
BACKGROUND
Besides routine serum markers of inflammatory diseases, the diagnostic potential of selected urinary proteins has not been fully exploited yet. Former studies revealed that urinary orosomucoid (u-ORM) might have complementary information in inflammatory disorders. Our aim was to develop and validate a fully automated method for u-ORM measurements and to evaluate its potential clinical impact on systemic inflammatory diseases.


METHODS
A particle-enhanced immune turbidimetric assay was validated for a Cobas 8000/c502 analyzer to determine u-ORM levels. Spot urine samples from 72 healthy individuals, 28 patients with Crohn's disease and 30 septic patients were studied.


RESULTS
Our assay time was 10 minutes and the detection limit of u-ORM was 0.02 mg/L. The intra- and inter-assay imprecision expressed as CV was less than 5%, and the recovery ranged between 95-103%. Within 10 to 60 years of age, a preliminary reference range for urinary orosomucoid/creatinine ratio (u-ORM/u-CREAT) was found to be 0.08 (0.01-0.24) mg/mmol [median (2.5-97.5 percentiles)]. Compared to controls, a five-fold increase of u-ORM/u-CREAT values in Crohn's disease and approximately a 240-fold increase in sepsis were observed.


CONCLUSIONS
We set up a fast, sensitive and precise turbidimetric approach for automated u-ORM determination. Our highly sensitive assay is ideal for routine u-ORM measurements and might be a potential novel laboratory test in the management of systemic inflammatory processes.
Department of Laboratory Medicine, University of Pécs Medical School, Pécs, Hungary; Department of Anaesthesiology and Intensive Therapy, University of Pécs Medical School, Pécs, Hungary.
Péter
P
Kustán
Department of Laboratory Medicine, University of Pécs Medical School, Pécs, Hungary.
Balázs
B
Szirmay
Department of Laboratory Medicine, University of Pécs Medical School, Pécs, Hungary.
Zoltán
Z
Horváth-Szalai
Department of Laboratory Medicine, University of Pécs Medical School, Pécs, Hungary.
Andrea
A
Ludány
Department of Laboratory Medicine, University of Pécs Medical School, Pécs, Hungary.
Ágnes
Á
Lakatos
Department of Anaesthesiology and Intensive Therapy, University of Pécs Medical School, Pécs, Hungary.
Diána
D
Mühl
Dako A/S, Glostrup, Denmark.
Per Hjort
PH
Christensen
Department of Laboratory Medicine, University of Pécs Medical School, Pécs, Hungary.
Attila
A
Miseta
Department of Laboratory Medicine, University of Pécs Medical School, Pécs, Hungary; Szentágothai Research Centre, Pécs, Hungary.
Gábor L
GL
Kovács
Department of Laboratory Medicine, University of Pécs Medical School, Pécs, Hungary; Szentágothai Research Centre, Pécs, Hungary.
Tamás
T
Kőszegi
D016022:Case-Control Studies; D003424:Crohn Disease / Q000652:urine; D006801:Humans; D057230:Limit of Detection; D009391:Nephelometry and Turbidimetry / Q000379:methods*; D009961:Orosomucoid / Q000652:urine*; D012016:Reference Values; D015203:Reproducibility of Results; D018805:Sepsis / Q000652:urine
D009961:Orosomucoid
biomarker; inflammation; orosomucoid; particle-enhanced turbidimetry; urine