Comparative Transcriptional Analysis of Loquat Fruit Identifies Major Signal Networks Involved in Fruit Development and Ripening Process.
Loquat (Eriobotrya japonicaLindl.) is an important non-climacteric fruit and rich in essential nutrients such as minerals and carotenoids. During fruit development and ripening, thousands of the differentially expressed genes (DEGs) from various metabolic pathways cause a series of physiological and biochemical changes. To better understand the underlying mechanism of fruit development, the Solexa/Illumina RNA-seq high-throughput sequencing was used to evaluate the global changes of gene transcription levels. More than 51,610,234 high quality reads from ten runs of fruit development were sequenced and assembled into 48,838 unigenes. Among 3256 DEGs, 2304 unigenes could be annotated to the Gene Ontology database. These DEGs were distributed into 119 pathways described in the Kyoto Encyclopedia of Genes and Genomes (KEGG) database. A large number of DEGs were involved in carbohydrate metabolism, hormone signaling, and cell-wall degradation. The real-time reverse transcription (qRT)-PCR analyses revealed that several genes related to cell expansion, auxin signaling and ethylene response were differentially expressed during fruit development. Other members of transcription factor families were also identified. There were 952 DEGs considered as novel genes with no annotation in any databases. These unigenes will serve as an invaluable genetic resource for loquat molecular breeding and postharvest storage.
Jiangsu Key Laboratory for Eco-Agricultural Biotechnology around Hongze Lake, College of Life Science, Huaiyin Normal University, Huai'an 223300, Jiangsu, China. loquathw@163.com.|Jiangsu Collaborative Innovation Center of Regional Modern Agriculture & Environmental Protection, Huaiyin Normal University, Huai'an 223300, Jiangsu, China. loquathw@163.com.
Huwei
H
Song
Jiangsu Key Laboratory for Eco-Agricultural Biotechnology around Hongze Lake, College of Life Science, Huaiyin Normal University, Huai'an 223300, Jiangsu, China. xxzhao2013@163.com.|Jiangsu Collaborative Innovation Center of Regional Modern Agriculture & Environmental Protection, Huaiyin Normal University, Huai'an 223300, Jiangsu, China. xxzhao2013@163.com.
Xiangxiang
X
Zhao
Jiangsu Key Laboratory for Eco-Agricultural Biotechnology around Hongze Lake, College of Life Science, Huaiyin Normal University, Huai'an 223300, Jiangsu, China. hu_weicheng@163.com.|Jiangsu Collaborative Innovation Center of Regional Modern Agriculture & Environmental Protection, Huaiyin Normal University, Huai'an 223300, Jiangsu, China. hu_weicheng@163.com.
Weicheng
W
Hu
Jiangsu Key Laboratory for Eco-Agricultural Biotechnology around Hongze Lake, College of Life Science, Huaiyin Normal University, Huai'an 223300, Jiangsu, China. wangxf@hytc.edu.cn.|Jiangsu Collaborative Innovation Center of Regional Modern Agriculture & Environmental Protection, Huaiyin Normal University, Huai'an 223300, Jiangsu, China. wangxf@hytc.edu.cn.
Xinfeng
X
Wang
Jiangsu Key Laboratory for Eco-Agricultural Biotechnology around Hongze Lake, College of Life Science, Huaiyin Normal University, Huai'an 223300, Jiangsu, China. shenting1019@163.com.|Jiangsu Collaborative Innovation Center of Regional Modern Agriculture & Environmental Protection, Huaiyin Normal University, Huai'an 223300, Jiangsu, China. shenting1019@163.com.
Ting
T
Shen
Jiangsu Key Laboratory for Eco-Agricultural Biotechnology around Hongze Lake, College of Life Science, Huaiyin Normal University, Huai'an 223300, Jiangsu, China. yanglm@hytc.edu.cn.|Jiangsu Collaborative Innovation Center of Regional Modern Agriculture & Environmental Protection, Huaiyin Normal University, Huai'an 223300, Jiangsu, China. yanglm@hytc.edu.cn.
Liming
L
Yang
D002338:Carotenoids / Q000096:biosynthesis; D031983:Eriobotrya / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D005030:Ethylenes / Q000378:metabolism; D005638:Fruit / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D018506:Gene Expression Regulation, Plant; D063990:Gene Ontology; D059014:High-Throughput Nucleotide Sequencing; D007210:Indoleacetic Acids / Q000378:metabolism; D053858:Metabolic Networks and Pathways / Q000235:genetics; D058977:Molecular Sequence Annotation; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D059467:Transcriptome
D005030:Ethylenes; D007210:Indoleacetic Acids; D010940:Plant Proteins; D002338:Carotenoids; C036216:ethylene
Eriobotrya japonica; expression profile; fruit development; ripening-regulated genes; transcriptome analysis