Laboratory tests for diagnosing and monitoring canine leishmaniasis.
Although several reviews on canine leishmaniasis have been published, none thoroughly described clinicopathologic abnormalities and their clinical usefulness. The aim of this review was to provide information concerning current diagnostic tests relevant for clinical pathologists and from a practical perspective. Specifically, in canine leishmaniasis, nonregenerative normocytic normochromic anemia, thrombocytopenia, or leukogram changes may be present. Clinical chemistry and urinalysis may indicate renal dysfunction (azotemia, decreased urine specific gravity, proteinuria) and an inflammatory/immune response (increased acute phase proteins [APP] or α2- and/or γ-globulins). Although a potential gammopathy is usually polyclonal, it may also appear oligo- or monoclonal, especially in dogs coinfected by other vector-borne pathogens. When lesions are accessible to fine-needle aspiration (lymphoadenomegaly, nodular lesions, joint swelling), cytology is strongly advised, as the presence of Leishmania amastigotes in a pattern of pyogranulomatous inflammation or lymphoplasmacytic hyperplasia is diagnostic. If the cytologic pattern is inconclusive, the parasite should be identified by histology/immunohistochemistry or PCR on surgical biopsies. Alternatively, cytology and PCR may be performed on bone marrow samples where amastigotes, along with erythroid hypoplasia, myeloid hyperplasia, plasmacytosis, or secondary dysmyelopoiesis can be observed. Dogs with overt leishmaniasis generally have high antibody titers, while low titers predominate in immunologically resistant infected dogs or in exposed dogs with no parasite confirmation. Quantitative serology is recommended in clinically suspect dogs as high-titer antibodies titers may confirm the clinical diagnosis. In confirmed and treated dogs, renal function and inflammatory/immune response variables should be periodically monitored.
Department of Veterinary Medicine, University of Milan, Milan, Italy.
Saverio
http://orcid.org/0000-0001-7117-7987
S
Paltrinieri
Unit of Vector-borne Diseases & International Health, Department of Infectious Diseases, Istituto Superiore di Sanità, Rome, Italy.
Luigi
L
Gradoni
Hospital Clínic Veterinari, Universitat Autònoma de Barcelona, Bellaterra, Spain.
Xavier
X
Roura
Medical Consultancy Services, TàXbiex, Malta.
Andrea
A
Zatelli
Clinic for Small Animal Internal Medicine, University of Zurich, Zurich, Switzerland.|Department of Animal Medicine, Production and Health, University of Padova, Padova, Italy.|Istituto Veterinario di Novara, Granozzo con Monticello, Italy.
Eric
E
Zini
D000818:Animals; D044963:Biopsy, Fine-Needle / Q000662:veterinary; D001853:Bone Marrow / Q000469:parasitology / Q000473:pathology; D004283:Dog Diseases / Q000175:diagnosis* / Q000276:immunology / Q000469:parasitology; D004285:Dogs; D007891:Leishmania / Q000166:cytology / Q000276:immunology / Q000302:isolation & purification*; D007896:Leishmaniasis / Q000175:diagnosis / Q000276:immunology / Q000469:parasitology / Q000662:veterinary*

Leishmania infantum
; Clinical usefulness; diagnosis; dog; follow-up