Association between polymorphisms in the promoter region of pri-miR-34b/c and risk of hepatocellular carcinoma.
Hepatocellular carcinoma (HCC) is a major cause of cancer-related deaths worldwide. MicroRNA-34 (miR-34) gene plays a key role in altering the apoptotic cycle and pathways of downstream cells, and therefore influences carcinogenesis. In this case-control study, we assessed the role of the pri-miR-34b/c rs4938723 polymorphism in HCC risk. The pri-miR-34b/c polymorphic genotype was determined in 286 patients with HCC and 572 controls using polymerase chain reaction-restriction fragment length polymorphism. The male gender (X2= 12.95, P < 0.001), regular alcohol consumption (X2= 16.81, P < 0.001), and a family history of cancer (X2= 11.88, P = 0.001) were associated with HCC risk. However, the age (t = 1.19, P = 0.12) and tobacco smoking habit (X2= 0.64, P = 0.42) of HCC patients were comparable to those of the controls. The TC (adjusted OR = 1.46, 95%CI = 1.06-2.01) and CC (adjusted OR = 3.07, 95%CI = 1.77-5.34) genotypes of pri-miR-34b/c rs4938723 were correlated with a higher risk of HCC compared to the TT genotype. Moreover, the TC+CC genotype was correlated with an increased risk of HCC compared to the TT genotype (adjusted OR = 1.64, 95%CI = 1.21-2.22). In the recessive model, the CC genotype of pri-miR-34b/c rs4938723 was significantly correlated with an elevated risk of HCC compared to the TT+TC genotype (adjusted OR = 2.50, 95%CI = 1.49-4.22). Further large-scale and multi-center studies are required to confirm these results.
Department of Gastroenterology, The First Affiliated Hospital, Zhejiang University, Hangzhou, China.|Department of Gastroenterology, The First People's Hospital of Wenling, Wenling, China.
L L
LL
Chen
Department of Geriatrics, Zhongshan Hospital, Fudan University, Shanghai, China.
Y
Y
Shen
Liver Cancer Institute and Zhongshan Hospital, Fudan University, Shanghai, China.
J B
JB
Zhang
Department of Cardiology, The First People's Hospital of Wenling, Wenling, China.
S
S
Wang
Central Laboratory, The First People's Hospital of Wenling, Wenling, China.
T
T
Jiang
Central Laboratory, The First People's Hospital of Wenling, Wenling, China.
M Q
MQ
Zheng
Department of Hepatobiliary Surgery, The First People's Hospital of Wenling, Wenling, China zhengzhijianz@163.com.
Z J
ZJ
Zheng
Department of Gastroenterology, The First Affiliated Hospital, Zhejiang University, Hangzhou, China.
C X
CX
Chen
D006528:Carcinoma, Hepatocellular / Q000235:genetics*; D016022:Case-Control Studies; D003710:Demography; D005260:Female; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics; D008297:Male; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D011401:Promoter Regions, Genetic; D012307:Risk Factors
C522425:MIRN34 microRNA, human; D035683:MicroRNAs