Lubrication synergy: Mixture of hyaluronan and dipalmitoylphosphatidylcholine (DPPC) vesicles.
Phospholipids and hyaluronan have been implied to fulfil important roles in synovial joint lubrication. Since both components are present in synovial fluid, self-assembly structures formed by them should also be present. We demonstrate by small angle X-ray scattering that hyaluronan associates with the outer shell of dipalmitoylphophatidylcholine (DPPC) vesicles in bulk solution. Further, we follow adsorption to silica from mixed hyaluronan/DPPC vesicle solution by Quartz Crystal Microbalance with Dissipation measurements. Atomic Force Microscope imaging visualises the adsorbed layer structure consisting of non-homogeneous phospholipid bilayer with hyaluronan/DPPC aggregates on top. The presence of these aggregates generates a long-range repulsive surface force as two such surfaces are brought together. However, the aggregates are easily deformed, partly rearranged into multilayer structures and partly removed from between the surfaces under high loads. These layers offer very low friction coefficient (<0.01), high load bearing capacity (≈23MPa), and self-healing ability. Surface bound DPPC/hyaluronan aggregates provide a means for accumulation of lubricating DPPC molecules on sliding surfaces.
KTH Royal Institute of Technology, School of Chemical Sciences and Engineering, Department of Chemistry, Surface and Corrosion Science, Drottning Kristinas Väg 51, SE-10044 Stockholm, Sweden. Electronic address: rajaka@kth.se.
Akanksha
A
Raj
KTH Royal Institute of Technology, School of Chemical Sciences and Engineering, Department of Chemistry, Surface and Corrosion Science, Drottning Kristinas Väg 51, SE-10044 Stockholm, Sweden. Electronic address: mwang@kth.se.
Min
M
Wang
Helmholtz Zentrum Geesthacht, Institute for Materials Research, Max-Planck Straße 1, 21502 Geesthacht, Germany. Electronic address: thomas.zander@hzg.de.
Thomas
T
Zander
Helmholtz Zentrum Geesthacht, Institute for Materials Research, Max-Planck Straße 1, 21502 Geesthacht, Germany. Electronic address: florian.wieland@hzg.de.
D C Florian
DCF
Wieland
KTH Royal Institute of Technology, School of Chemical Sciences and Engineering, Department of Chemistry, Surface and Corrosion Science, Drottning Kristinas Väg 51, SE-10044 Stockholm, Sweden. Electronic address: liuxia@kemi.dtu.dk.
Xiaoyan
X
Liu
KTH Royal Institute of Technology, School of Chemical Sciences and Engineering, Department of Chemistry, Surface and Corrosion Science, Drottning Kristinas Väg 51, SE-10044 Stockholm, Sweden. Electronic address: junxue@kth.se.
Junxue
J
An
Helmholtz Zentrum Geesthacht, Institute for Materials Research, Max-Planck Straße 1, 21502 Geesthacht, Germany. Electronic address: vasyl.haramus@hzg.de.
Vasil M
VM
Garamus
Helmholtz Zentrum Geesthacht, Institute for Materials Research, Max-Planck Straße 1, 21502 Geesthacht, Germany. Electronic address: regine.willumeit@hzg.de.
Regine
R
Willumeit-Römer
KTH Royal Institute of Technology, Albanova Campus, Department of Applied Physics, Roslagstullsbacken 21, SE-10044 Stockholm, Sweden. Electronic address: fielden@kth.se.
Matthew
M
Fielden
KTH Royal Institute of Technology, School of Chemical Sciences and Engineering, Department of Chemistry, Surface and Corrosion Science, Drottning Kristinas Väg 51, SE-10044 Stockholm, Sweden; SP Technical Research Institute of Sweden, SP Chemistry, Materials and Surfaces, Box 5607, SE-114 86 Stockholm, Sweden. Electronic address: percl@kth.se.
Per M
PM
Claesson
KTH Royal Institute of Technology, School of Chemical Sciences and Engineering, Department of Chemistry, Surface and Corrosion Science, Drottning Kristinas Väg 51, SE-10044 Stockholm, Sweden; SP Technical Research Institute of Sweden, SP Chemistry, Materials and Surfaces, Box 5607, SE-114 86 Stockholm, Sweden. Electronic address: andra@kth.se.
Andra
A
Dėdinaitė
D015060:1,2-Dipalmitoylphosphatidylcholine / Q000737:chemistry*; D000327:Adsorption; D001696:Biomechanical Phenomena; D017276:Friction; D006820:Hyaluronic Acid / Q000737:chemistry*; D008051:Lipid Bilayers / Q000737:chemistry*; D008956:Models, Chemical; D058525:Quartz Crystal Microbalance Techniques; D012822:Silicon Dioxide / Q000737:chemistry*; D012996:Solutions
D008051:Lipid Bilayers; D012996:Solutions; D015060:1,2-Dipalmitoylphosphatidylcholine; D012822:Silicon Dioxide; D006820:Hyaluronic Acid
AFM imaging; Adsorption; Dipalmitoylphosphatidylcholine (DPPC); Friction; Hyaluronan; Lubrication; QCM-D; Self-assembly; Small-angle X-ray scattering; Surface forces