Rapid and Quantitative Determination of S-Adenosyl-L-Methionine in the Fermentation Process by Surface-Enhanced Raman Scattering.
Concentrations of S-Adenosyl-L-Methionine (SAM) in aqueous solution and fermentation liquids were quantitatively determined by surface-enhanced Raman scattering (SERS) and verified by high-pressure liquid chromatography (HPLC). The Ag nanoparticle/silicon nanowire array substrate was fabricated and employed as an active SERS substrate to indirectly measure the SAM concentration. The linear relationship between the integrated intensity of peak centered at ~2920 cm-1in SERS spectra and the SAM concentration was established, and the limit of detections of SAM concentrations was analyzed to be ~0.1 g/L. The concentration of SAM in real solution could be predicted by the linear relationship and verified by the HPLC detection method. The relative deviations (δ) of the predicted SAM concentration are less than 13% and the correlation coefficient is 0.9998. Rolling-Circle Filter was utilized to subtract fluorescence background and the optimal results were obtained when the radius of the analyzing circle is 650 cm-1.
Beijing Bioprocess Key Laboratory, Beijing University of Chemical Technology, Beijing 100029, China; College of Science, Beijing University of Chemical Technology, Beijing 100029, China.
Hairui
H
Ren
Beijing Bioprocess Key Laboratory, Beijing University of Chemical Technology, Beijing 100029, China; College of Science, Beijing University of Chemical Technology, Beijing 100029, China.
Zhaoyang
Z
Chen
Beijing Bioprocess Key Laboratory, Beijing University of Chemical Technology, Beijing 100029, China; College of Science, Beijing University of Chemical Technology, Beijing 100029, China.
Xin
X
Zhang
Engineering Research Center for Semiconductor Integrated Technology, Institute of Semiconductors, Chinese Academy of Sciences, Beijing 100083, China.
Yongmei
Y
Zhao
Beijing Bioprocess Key Laboratory, Beijing University of Chemical Technology, Beijing 100029, China.
Zheng
0000-0002-8984-4837
Z
Wang
Analytical and Testing Center, Beijing Normal University, Beijing 100875, China.
Zhenglong
Z
Wu
Beijing Bioprocess Key Laboratory, Beijing University of Chemical Technology, Beijing 100029, China; College of Science, Beijing University of Chemical Technology, Beijing 100029, China.
Haijun
0000-0001-7290-228X
H
Xu