Electrochemical Biosensor for the Detection of Glycated Albumin.
Alzheimer's disease (AD) is the most common form of dementia. The process of AD can begin 20 years before any symptom of cognitive loss. Thus, the development of systems for early diagnosis and prevention is very important. The mechanism of AD is still under debate. Nevertheless, higher levels of glycated albumin in cerebrospinal fluid and plasma are observed in AD patients. Therefore, glycated albumin could be a biomarker of AD development.



Electrochemical biosensor for direct determination of glycated albumin was based on thiol derivative of pentetic acid (DTPA) complex with Cu(II) created on gold electrode surface. His-tagged domains of Receptors for Advanced Glycation End Products (RAGE) were applied as analytical active element for glycated albumin recognition. The binding of glycated albumin by His6- RAGE domains was monitored using Osteryoung square - wave voltammetry.



Electrodes modified with His6 - RAGE VC1 natural domain generated decrease of Cu(II) redox currents in the presence of glycated albumin. Human albumin, Aβ 1-40 and S100B protein caused negligible influence on biosensors responses towards glycated albumin. The detection limits were: 2.3 pM, 1.1 pM, 2.9 pM and 3.1 pM in the presence of: buffer, buffer + albumin, buffer + S100B, buffer + Aβ1-40 , respectively.



The presented electrochemical biosensor was successfully applied for the determination of glycated albumin. Considering analytical parameters such as good selectivity and sensitivity in pM range, biosensor could be recommended as an analytical tool for medical samples analysis.
Edyta
E
Mikula
Aleksandra
A
Wyslouch-Cieszynska
Liliya
L
Zhukova
Peter
P
Verwilst
Wim
W
Dehaen
Jerzy
J
Radecki
Institute of Animal Reproduction and Food Research Polish Academy of Science, Tuwima 10, 10-748 Olsztyn, Poland.
Hanna
H
Radecka
D000951:Antigens, Neoplasm / Q000737:chemistry / Q000235:genetics; D015374:Biosensing Techniques; D001774:Blood Chemical Analysis / Q000295:instrumentation; D003300:Copper; D055664:Electrochemical Techniques / Q000295:instrumentation*; D004566:Electrodes; D004867:Equipment Design; D017127:Glycation End Products, Advanced; D006046:Gold; D006801:Humans; D020928:Mitogen-Activated Protein Kinases / Q000737:chemistry / Q000235:genetics; D012709:Serum Albumin / Q000737:chemistry*; D000093423:Glycated Serum Albumin
D000951:Antigens, Neoplasm; D017127:Glycation End Products, Advanced; D012709:Serum Albumin; D006046:Gold; D003300:Copper; C498978:MOK protein, human; D020928:Mitogen-Activated Protein Kinases; D000093423:Glycated Serum Albumin
Alzheimer’s disease; His-tagged RAGE domains; electrochemical
biosensor; glycated albumin; pentetic acid-Cu(II) complex; potential AD biomarkers.; redox active monolayer 