Two-step cultivation for production of astaxanthin in Chlorella zofingiensis using a patented energy-free rotating floating photobioreactor (RFP).
In the present study, high light and nitrogen starvation with glucose-fed to the culture was found efficient to induce astaxanthin accumulation in Chlorella zofingiensis. Therefore, a two-step cultivation strategy including high biomass yield fermentation and outdoor induction with an energy-free RFP was conducted. During the fermentation, the highest cell density of 98.4gL-1and astaxanthin yield of 73.3mgL-1were achieved, which were higher than those so far reported in C. zofingiensis. During the outdoor induction, astaxanthin content was further increased by 1.5-fold leading to the highest astaxanthin productivity of 5.26mgL-1day-1under an optimal dilution of 5-fold. Our work thus provided an effective two-step cultivation strategy for production of astaxanthin by C. zofingiensis.
Institute for Food & Bioresource Engineering, College of Engineering, Peking University, Beijing 100871, China; Department of Microbiology and Immunology, Yong Loo Lin School of Medicine, National University of Singapore, 5 Science Drive 2, Singapore 117597, Singapore; BIC-ESAT, College of Engineering, Peking University, Beijing 100871, China.
Zhao
Z
Zhang
Department of Microbiology and Immunology, Yong Loo Lin School of Medicine, National University of Singapore, 5 Science Drive 2, Singapore 117597, Singapore.
Jim Junhui
JJ
Huang
Institute for Food & Bioresource Engineering, College of Engineering, Peking University, Beijing 100871, China; Department of Microbiology and Immunology, Yong Loo Lin School of Medicine, National University of Singapore, 5 Science Drive 2, Singapore 117597, Singapore; BIC-ESAT, College of Engineering, Peking University, Beijing 100871, China.
Dongzhe
D
Sun
Department of Microbiology and Immunology, Yong Loo Lin School of Medicine, National University of Singapore, 5 Science Drive 2, Singapore 117597, Singapore.
Yuankun
Y
Lee
Institute for Food & Bioresource Engineering, College of Engineering, Peking University, Beijing 100871, China; BIC-ESAT, College of Engineering, Peking University, Beijing 100871, China. Electronic address: sfchencoe@pku.edu.cn.
Feng
F
Chen
D018533:Biomass; D002338:Carotenoids / Q000302:isolation & purification / Q000378:metabolism; D002708:Chlorella / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D003624:Darkness; D005285:Fermentation; D005947:Glucose / Q000378:metabolism; D008027:Light; D009584:Nitrogen; D010330:Patents as Topic; D058433:Photobioreactors; D024341:Xanthophylls / Q000378:metabolism
D024341:Xanthophylls; D002338:Carotenoids; C005948:astaxanthine; D005947:Glucose; D009584:Nitrogen
Astaxanthin; Chlorella zofingiensis; Energy-free RFP; Microalgae; Two-step cultivation