Streptomyces xiangtanensis sp. nov., isolated from a manganese-contaminated soil.
An actinomycete strain, designated strain LUSFXJT, was isolated from a soil sample obtained near the Xiangtan Manganese Mine, Central-South China and characterised using a polyphasic taxonomic approach. The 16S rRNA gene sequence-based phylogenetic analysis indicated that this strain belongs to the genus Streptomyces. The DNA-DNA relatedness between this strain and two closely related type strains, Streptomyces echinatus CGMCC 4.1642Tand Streptomyces lanatus CGMCC 4.137T, were 28.7 ± 0.4 and 19.9 ± 2.0%, respectively, values which are far lower than the 70% threshold for the delineation of a novel prokaryotic species. The DNA G+C content of strain LUSFXJTis 75.0 mol%. Chemotaxonomic analysis revealed that the menaquinones of strain LUSFXJTare MK-9(H6), MK-9(H8), MK-9(H2) and MK-8(H8). The polar lipid profile of strain LUSFXJTwas found to contain diphosphatidylglycerol and an unidentified polar lipid. The major cellular fatty acids were identified as iso-C15:0, anteiso-C15:0, iso-C16:0, C16:0and Summed feature 3. Strain LUSFXJTwas found to contain meso-diaminopimelic acid as the diagnostic cell wall diamino acid and the whole cell hydrolysates were found to be rich in ribose, mannose and glucose. Based on phenotypic, phylogenetic and chemotaxonomic characteristics, it is concluded that strain LUSFXJTrepresents a novel species of the genus Streptomyces, for which the name S. xiangtanensis sp. nov. is proposed. The type strain is LUSFXJT(=GDMCC 4.133T= KCTC 39829T).
School of Life Science, Hunan University of Science and Technology, Xiangtan, 411201, People's Republic of China.|Key Laboratory of Ecological Remediation and Safe Utilization of Heavy Metal-Polluted Soils, College of Hunan Province, Xiangtan, 411201, People's Republic of China.
Ping
P
Mo
Biological Resources Institute, Jiangxi Academy of Sciences, Nanchang, 330096, People's Republic of China.
Yi-Zun
YZ
Yu
School of Life Science, Hunan University of Science and Technology, Xiangtan, 411201, People's Republic of China.|Key Laboratory of Ecological Remediation and Safe Utilization of Heavy Metal-Polluted Soils, College of Hunan Province, Xiangtan, 411201, People's Republic of China.
Jia-Rong
JR
Zhao
School of Life Science, Hunan University of Science and Technology, Xiangtan, 411201, People's Republic of China. xtgojian@126.com.|Key Laboratory of Ecological Remediation and Safe Utilization of Heavy Metal-Polluted Soils, College of Hunan Province, Xiangtan, 411201, People's Republic of China. xtgojian@126.com.
Jian
J
Gao
D002473:Cell Wall / Q000737:chemistry; D002681:China; D008345:Manganese / Q000737:chemistry*; D010641:Phenotype; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012965:Sodium Chloride / Q000378:metabolism; D012987:Soil / Q000737:chemistry; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000737:chemistry*; D013045:Species Specificity; D013302:Streptomyces / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology
D012336:RNA, Ribosomal, 16S; D012987:Soil; D012989:Soil Pollutants; D008345:Manganese; D012965:Sodium Chloride
Phylogeny; Polyphasic taxonomy; Streptomyces xiangtanensis sp. nov.