Intestinal alkaline phosphatase and sodium butyrate may be beneficial in attenuating LPS-induced intestinal inflammation.
In this study, we evaluated the effect of intestinal alkaline phosphatase (IAP) and sodium butyrate (NaBu) on lipopolysaccharide (LPS)-induced intestinal inflammation. Intestinal alkaline phosphatase and RelA/p65 (NF-κB) gene expressions in porcine jejunum explants were evaluated following exposure to sodium butyrate (NaBu) and essential oil from Brazilian red pepper (EO), alone or in combination with NaBu, as well as exogenous IAP with or without LPS challenge. Five piglets weighing approximately 20 kg each were sacrificed, and their jejunum were extracted. The tissues were segmented into 10 parts, which were exposed to 10 treatments. Gene expressions of IAP and RelA/p65 (NF-κB) in jejunal explants were evaluated via RT-PCR. We found that EO, NaBu, and exogenous IAP were able to up-regulate endogenous IAP and enhance RelA/p65 (NF-κB) gene expression. However, only NaBu and exogenous IAP down-regulated LPS-induced inflammatory response via RelA/p65 (NF-κB). In conclusion, we demonstrated that exogenous IAP and NaBu may be beneficial in attenuating LPS-induced intestinal inflammation.
Pontifícia Universidade Católica do Paraná, Curitiba, PR, Brasil diegobmelo@hotmail.com.|Purdue University, West Lafayette, IN, USA diegobmelo@hotmail.com.
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AD
Melo
Universidade Federal de Lavras, Lavras, MG, Brasil.
H
H
Silveira
Purdue University, West Lafayette, IN, USA.
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C
Bortoluzzi
Universidade Federal de Lavras, Lavras, MG, Brasil.
L J
LJ
Lara
Universidade Federal Rural da Amazônia, Paragominas, PA, Brasil.
C A P
CA
Garbossa
Universidade do Estado de Santa Catarina, Lages, SC, Brasil.
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Preis
Pontifícia Universidade Católica do Paraná, Curitiba, PR, Brasil.
L B
LB
Costa
Purdue University, West Lafayette, IN, USA.
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MH
Rostagno
D000469:Alkaline Phosphatase / Q000235:genetics / Q000494:pharmacology*; D000818:Animals; D020148:Butyric Acid / Q000494:pharmacology*; D002417:Cattle; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D007249:Inflammation / Q000201:enzymology / Q000235:genetics / Q000473:pathology*; D007583:Jejunum / Q000187:drug effects / Q000201:enzymology* / Q000473:pathology*; D008070:Lipopolysaccharides; D009822:Oils, Volatile / Q000494:pharmacology; D060888:Real-Time Polymerase Chain Reaction; D034421:Sus scrofa; D051996:Transcription Factor RelA / Q000378:metabolism; D015854:Up-Regulation / Q000187:drug effects
D008070:Lipopolysaccharides; D009822:Oils, Volatile; D051996:Transcription Factor RelA; D020148:Butyric Acid; D000469:Alkaline Phosphatase