Methylation-sensitive amplified polymorphism analysis of Verticillium wilt-stressed cotton (Gossypium).
In this study, a methylation-sensitive amplification polymorphism analysis system was used to analyze DNA methylation level in three cotton accessions. Two disease-sensitive near-isogenic lines, PD94042 and IL41, and one disease-resistant Gossypium mustelinum accession were exposed to Verticillium wilt, to investigate molecular disease resistance mechanisms in cotton. We observed multiple different DNA methylation types across the three accessions following Verticillium wilt exposure. These included hypomethylation, hypermethylation, and other patterns. In general, the global DNA methylation level was significantly increased in the disease-resistant accession G. mustelinum following disease exposure. In contrast, there was no significant difference in the disease-sensitive accession PD94042, and a significant decrease was observed in IL41. Our results suggest that disease-resistant cotton might employ a mechanism to increase methylation level in response to disease stress. The differing methylation patterns, together with the increase in global DNA methylation level, might play important roles in tolerance to Verticillium wilt in cotton. Through cloning and analysis of differently methylated DNA sequences, we were also able to identify several genes that may contribute to disease resistance in cotton. Our results revealed the effect of DNA methylation on cotton disease resistance, and also identified genes that played important roles, which may shed light on the future cotton disease-resistant molecular breeding.
State Key Laboratory of Cotton Biology; Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang, Henan Province, China.|School of Life Sciences, Nantong University, Nantong, Jiangsu Province, China.|Agricultural Science Institute of Coastal Region of Jiangsu, Yancheng, Jiangsu Province, China.
W
W
Wang
School of Life Sciences, Nantong University, Nantong, Jiangsu Province, China.
M
M
Zhang
Cotton Sciences Research Institute of Hunan/National Hybrid Cotton Research Promotion Center, Changde, Hunan Province, China.
H D
HD
Chen
School of Life Sciences, Nantong University, Nantong, Jiangsu Province, China.
X X
XX
Cai
School of Life Sciences, Nantong University, Nantong, Jiangsu Province, China.
M L
ML
Xu
School of Life Sciences, Nantong University, Nantong, Jiangsu Province, China.
K Y
KY
Lei
School of Life Sciences, Nantong University, Nantong, Jiangsu Province, China.
J H
JH
Niu
School of Life Sciences, Nantong University, Nantong, Jiangsu Province, China.
L
L
Deng
School of Life Sciences, Nantong University, Nantong, Jiangsu Province, China.
J
J
Liu
Agricultural Science Institute of Coastal Region of Jiangsu, Yancheng, Jiangsu Province, China.
Z J
ZJ
Ge
State Key Laboratory of Cotton Biology; Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang, Henan Province, China.
S X
SX
Yu
School of Life Sciences, Nantong University, Nantong, Jiangsu Province, China bhwangzm@126.com.
B H
BH
Wang
D019175:DNA Methylation; D060467:Disease Resistance; D017343:Genes, Plant; D003368:Gossypium / Q000235:genetics*; D000069600:Plant Breeding; D010935:Plant Diseases / Q000235:genetics*; D011110:Polymorphism, Genetic; D020172:Verticillium / Q000235:genetics / Q000378:metabolism