Cotton Stalk Pretreatment Using Daedalea flavida, Phlebia radiata, and Flavodon flavus: Lignin Degradation, Cellulose Recovery, and Enzymatic Saccharification.
Lignocellulolytic enzyme activities of selective fungi Daedalea flavida MTCC 145 (DF-2), Phlebia radiata MTCC 2791 (PR), and non-selective fungus Flavodon flavus MTCC 168 (FF) were studied for pretreatment of cotton stalks. Simultaneous productions of high LiP and laccase activities by DF-2 during early phase of growth were effective for lignin degradation 27.83 ± 1.25 % (w/w of lignin) in 20-day pretreatment. Production of high MnP activity without laccase in the early growth phase of PR was ineffective and delayed lignin degradation 24.93 ± 1.53 % in 25 days due to laccase production at later phase. With no LiP activity, low activities of MnP and laccase by FF yielded poor lignin degradation 15.09 ± 0.6 % in 20 days. Xylanase was predominant cellulolytic enzyme produced by DF-2, resulting hemicellulose as main carbon and energy source with 83 % of cellulose recovery after 40 days of pretreatment. The glucose yield improved more than two fold from 20-day DF-2 pretreated cotton stalks after enzymatic saccharification.
Department of Biotechnology, Dr B R A National Institute of Technology, Jalandhar, Punjab, 144011, India.
Harmanpreet
H
Meehnian
Department of Biotechnology, Dr B R A National Institute of Technology, Jalandhar, Punjab, 144011, India. janaak@nitj.ac.in.
Asim K
AK
Jana
D018533:Biomass; D001709:Biotechnology / Q000379:methods*; D002482:Cellulose / Q000378:metabolism*; D004798:Enzymes / Q000378:metabolism*; D003368:Gossypium / Q000737:chemistry*; D008031:Lignin / Q000378:metabolism*; D020072:Polyporales / Q000201:enzymology / Q000254:growth & development / Q000378:metabolism*
D004798:Enzymes; C036909:lignocellulose; D002482:Cellulose; D008031:Lignin
Cellulose recovery; Cotton stalks; Enzymatic saccharification; Lignin degradation; Lignocellulolytic enzymes