Control of sexuality by thesk1-encoded UDP-glycosyltransferase of maize.
Sex determination in maize involves the production of staminate and pistillate florets from an initially bisexual floral meristem. Pistil elimination in staminate florets requires jasmonic acid signaling, and functional pistils are protected by the action of thesilkless 1(sk1) gene. Thesk1gene was identified and found to encode a previously uncharacterized family 1 uridine diphosphate glycosyltransferase that localized to the plant peroxisomes. Constitutive expression of ansk1transgene protected all pistils in the plant, causing complete feminization, a gain-of-function phenotype that operates by blocking the accumulation of jasmonates. The segregation of ansk1transgene was used to effectively control the production of pistillate and staminate inflorescences in maize plants.
Department of Molecular, Cellular and Developmental Biology, Yale University, New Haven, CT 06520-8104, USA.
Andrew P
AP
Hayward
Department of Molecular, Cellular and Developmental Biology, Yale University, New Haven, CT 06520-8104, USA.
Maria A
MA
Moreno
Department of Molecular, Cellular and Developmental Biology, Yale University, New Haven, CT 06520-8104, USA.
Thomas P
TP
Howard
Department of Cell and Molecular Biology, University of Rhode Island, Kingston, RI 02892, USA.
Joel
J
Hague
Department of Cell and Molecular Biology, University of Rhode Island, Kingston, RI 02892, USA.
Kimberly
K
Nelson
Department of Molecular, Cellular and Developmental Biology, Yale University, New Haven, CT 06520-8104, USA.
Christopher
C
Heffelfinger
Department of Molecular, Cellular and Developmental Biology, Yale University, New Haven, CT 06520-8104, USA.
Sandra
S
Romero
Department of Cell and Molecular Biology, University of Rhode Island, Kingston, RI 02892, USA.
Albert P
AP
Kausch
Neuchâtel Platform of Analytical Chemistry, University of Neuchâtel, Avenue de Bellevaux 51, 2000 Neuchâtel, Switzerland.
Gaétan
G
Glauser
Max Planck Institute for Plant Breeding Research, D-50829 Cologne, Germany.
Ivan F
0000-0001-7080-3384
IF
Acosta
Department of Cell and Molecular Biology, University of Rhode Island, Kingston, RI 02892, USA.
John P
JP
Mottinger
Department of Molecular, Cellular and Developmental Biology, Yale University, New Haven, CT 06520-8104, USA.
Stephen L
SL
Dellaporta
D003517:Cyclopentanes / Q000378:metabolism; D016695:Glycosyltransferases / Q000096:biosynthesis / Q000235:genetics; D056626:Inflorescence / Q000201:enzymology / Q000235:genetics; D054883:Oxylipins / Q000378:metabolism; D020675:Peroxisomes / Q000201:enzymology / Q000235:genetics; D010940:Plant Proteins / Q000096:biosynthesis / Q000235:genetics; D003313:Zea mays / Q000201:enzymology / Q000235:genetics
D003517:Cyclopentanes; D054883:Oxylipins; D010940:Plant Proteins; C011006:jasmonic acid; D016695:Glycosyltransferases
Floral development; UDP-glycosyltransferase; jasmonic acid; plant hybrids; sex determination; silkless 1 gene