Proteomic Dissection of Seed Germination and Seedling Establishment inBrassica napus.
The success of seed germination and establishment of a normal seedling are key determinants of plant species propagation. At present, only a few studies have focused on the genetic control of seed germination by using a proteomic approach inBrassica napus. In the present study, the protein expression pattern of seed germination was investigated using differential fluorescence two-dimensional gel electrophoresis inB. napus. One hundred and thirteen differentially expressed proteins (DEPs) that were mainly involved in storage (23.4%), energy metabolism (18.9%), protein metabolism (16.2%), defense/disease (12.6%), seed maturation (11.7%), carbohydrate metabolism (4.5%), lipid metabolism (4.5%), amino acids metabolism (3.6%), cell growth/division (3.6%), and some unclear functions (2.7%) were observed by proteomic analysis. Seventeen genes corresponding to 11 DEPs were identified within or near the associated linkage disequilibrium regions related to seed germination and vigor quantitative traits reported inB. napusin previous studies. The expression pattern of proteins showed that heterotrophic metabolism could be activated in the process of seed germination and that the onset of defense mechanisms might start during seed germination. These findings will help generate a more in-depth understanding of the mobilization of seed storage reserves and regulation mechanisms of the germination process inB. napus.
Department of Biotechnology, College of Life Science and Technology, Huazhong University of Science and TechnologyWuhan, China; Hubei Collaborative Innovation Center for the Characteristic Resources Exploitation of Dabie Mountains, Huanggang Normal UniversityHuanggang, China.
Jianwei
J
Gu
Department of Biotechnology, College of Life Science and Technology, Huazhong University of Science and Technology Wuhan, China.
Hongbo
H
Chao
Department of Biotechnology, College of Life Science and Technology, Huazhong University of Science and Technology Wuhan, China.
Lu
L
Gan
Department of Biotechnology, College of Life Science and Technology, Huazhong University of Science and Technology Wuhan, China.
Liangxing
L
Guo
Department of Biotechnology, College of Life Science and Technology, Huazhong University of Science and Technology Wuhan, China.
Kai
K
Zhang
Hybrid Rapeseed Research Center of Shaanxi Province, Shaanxi Rapeseed Branch of National Centre for Oil Crops Genetic Improvement Yangling, China.
Yonghong
Y
Li
Hybrid Rapeseed Research Center of Shaanxi Province, Shaanxi Rapeseed Branch of National Centre for Oil Crops Genetic Improvement Yangling, China.
Hao
H
Wang
Department of Biotechnology, College of Life Science and Technology, Huazhong University of Science and Technology Wuhan, China.
Nadia
N
Raboanatahiry
Department of Biotechnology, College of Life Science and Technology, Huazhong University of Science and TechnologyWuhan, China; Hubei Collaborative Innovation Center for the Characteristic Resources Exploitation of Dabie Mountains, Huanggang Normal UniversityHuanggang, China.
Maoteng
M
Li
Brassica napus; proteomic analysis; seed germination; storage reserves