In-vitro responses of T lymphocytes to poly(butylene succinate) based biomaterials.
BACKGROUND
Polybutylene succinate (PBSu) and PBSu/β-tricalcium phosphate (TCP) composites are biocompatible and good candidates as bone graft materials. However, little is known about the responses of T lymphocytes to these biomaterials, which play an important role in the success of bone grafting.


METHODS
Activated T lymphocytes were cultured onto 32 mm diameter films (PBSu/TCP films), that had previously been placed in 6-well culture plates, for 8, 24 and 72 hours. A plastic-well culture plate was used as a control surface. The effects of PBSu-based biomaterials on T lymphocytes were examined by the using flow cytometry and reverse-transcription polymerase chain reaction.


RESULTS
These biomaterials were non-toxic to T lymphocytes, allowing their normal DNA synthesis and activation. All materials induced only transient activation of T lymphocytes, which existed no longer than 72 hours. Proportions of four main CD4/CD8 T lymphocyte subpopulations were not affected by these biomaterials. Moreover, PBSu and PBSu/TCP significantly suppressed the expression of IL-1β and IL-6 genes by 15-35% and 21-26%, respectively. In contrast, a PBSu/TCP composite (at PBSu:TCP=60:40) significantly stimulated the expression of IL-10 and IL-13 genes by 17% and 19%, respectively.


CONCLUSIONS
PBSu and PBSu/TCP composites were non-toxic to T lymphocytes and did not induce unfavorable responses of T lymphocytes. The tested biomaterials down-regulated key proinflammatory cytokine genes and up-regulated anti-inflammatory cytokine genes in T lymphocytes. These suggest that the biomaterials studied are good candidates as bone graft materials.
Faculty of Dentistry, Thammasat University, Pathumthani, Thailand.
Montree
M
Toso
Faculty of Dentistry, Thammasat University, Pathumthani, Thailand.
Somying
S
Patntirapong
Biomedical Engineering Research Unit, National Metal and Materials Technology Center, Pathumthani, Thailand.
Wanida
W
Janvikul
Faculty of Dentistry, Thammasat University, Pathumthani, Thailand - s-wrch@tu.ac.th.
Weerachai
W
Singhatanadgit
D001672:Biocompatible Materials / Q000494:pharmacology* / Q000633:toxicity; D018786:Bone Substitutes / Q000494:pharmacology* / Q000633:toxicity; D002072:Butylene Glycols / Q000494:pharmacology* / Q000633:toxicity; D015496:CD4-Positive T-Lymphocytes / Q000187:drug effects / Q000378:metabolism; D018414:CD8-Positive T-Lymphocytes / Q000187:drug effects / Q000378:metabolism; D002478:Cells, Cultured; D016207:Cytokines / Q000096:biosynthesis / Q000235:genetics; D004261:DNA Replication / Q000187:drug effects; D003764:Dental Materials / Q000494:pharmacology* / Q000633:toxicity; D006801:Humans; D007249:Inflammation / Q000235:genetics; D008213:Lymphocyte Activation / Q000187:drug effects; D008422:Materials Testing; D011108:Polymers / Q000494:pharmacology* / Q000633:toxicity; D012333:RNA, Messenger / Q000096:biosynthesis; D016176:T-Lymphocyte Subsets / Q000187:drug effects* / Q000378:metabolism
D001672:Biocompatible Materials; D018786:Bone Substitutes; D002072:Butylene Glycols; D016207:Cytokines; D003764:Dental Materials; D011108:Polymers; D012333:RNA, Messenger; C089797:bionole