Characterization of a soybean mosaic virus variant causing different diseases in Glycine max and Nicotiana benthamiana.
We discovered a soybean mosaic virus (SMV) variant (4278-1) that caused systemic infections in Nicotiana benthamiana plants, resulting in stem stunting and leaf shriveling. The virus had a particle morphology and incubation period similar to those of other SMV isolates but differed from them in the leaf symptoms it caused when infecting soybean and N. benthamiana. The genome of this variant consisted of a 9994-nt single-stranded RNA, which was different from most of the other known SMV isolates (approximately 9600 nt). Interestingly, we found evidence that two recombination events (nt 1-476 and nt 1145-1349) had occurred between 4278-1 and a watermelon mosaic virus analogue (WMV analogue), in the 5' untranslated region and the P1 cistron.
Soybean Research Institute, Nanjing Agricultural University, Nanjing, Jiangsu, 210095, China.|National Center for Soybean Improvement, Ministry of Agriculture, Nanjing, Jiangsu, 210095, China.|Key Laboratory of Biology and Genetic Improvement of Soybean (General), Ministry of Agriculture, Nanjing, Jiangsu, 210095, China.|National Key Laboratory for Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing, Jiangsu, 210095, China.|Jiangsu Collaborative Innovation Center for Modern Crop Production, Nanjing Agricultural University, Nanjing, Jiangsu, 210095, China.
Hua
H
Jiang
Soybean Research Institute, Nanjing Agricultural University, Nanjing, Jiangsu, 210095, China.|National Center for Soybean Improvement, Ministry of Agriculture, Nanjing, Jiangsu, 210095, China.|Key Laboratory of Biology and Genetic Improvement of Soybean (General), Ministry of Agriculture, Nanjing, Jiangsu, 210095, China.|National Key Laboratory for Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing, Jiangsu, 210095, China.|Jiangsu Collaborative Innovation Center for Modern Crop Production, Nanjing Agricultural University, Nanjing, Jiangsu, 210095, China.
Kai
K
Li
Department of Plant Pathology, Nanjing Agricultural University, Nanjing, 210095, Jiangsu, China. ddou@njau.edu.cn.
Daolong
D
Dou
Soybean Research Institute, Nanjing Agricultural University, Nanjing, Jiangsu, 210095, China. sri@njau.edu.cn.|National Center for Soybean Improvement, Ministry of Agriculture, Nanjing, Jiangsu, 210095, China. sri@njau.edu.cn.|Key Laboratory of Biology and Genetic Improvement of Soybean (General), Ministry of Agriculture, Nanjing, Jiangsu, 210095, China. sri@njau.edu.cn.|National Key Laboratory for Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing, Jiangsu, 210095, China. sri@njau.edu.cn.|Jiangsu Collaborative Innovation Center for Modern Crop Production, Nanjing Agricultural University, Nanjing, Jiangsu, 210095, China. sri@njau.edu.cn.
Junyi
http://orcid.org/0000-0001-6222-2010
J
Gai
D020121:5' Untranslated Regions; D018745:Genome, Plant; D010802:Phylogeny; D010935:Plant Diseases / Q000145:classification / Q000821:virology*; D018515:Plant Leaves / Q000821:virology; D018547:Plant Stems / Q000821:virology; D017800:Potyvirus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D012367:RNA, Viral / Q000235:genetics; D011995:Recombination, Genetic; D013025:Glycine max / Q000821:virology*; D013045:Species Specificity; D014026:Nicotiana / Q000821:virology*; D014764:Viral Proteins / Q000235:genetics*
D020121:5' Untranslated Regions; D012367:RNA, Viral; D014764:Viral Proteins