ZHX1 Promotes the Proliferation, Migration and Invasion of Cholangiocarcinoma Cells.
Zinc-fingers and homeoboxes 1 (ZHX1) is a transcription repressor that has been associated with the progressions of hepatocellular carcinoma, gastric cancer, and breast cancer. However, the functional roles of ZHX1 in cholangiocarcinoma (CCA) have not been determined. We investigated the expression and roles of ZHX1 during the proliferation, migration, and invasion of CCA cells. In silico analysis and immunohistochemical studies showed amplification and overexpression of ZHX1 in CCA tissues. Furthermore, ZHX1 knockdown using specific siRNAs decreased CCA cell proliferation, migration, and invasion, whereas ZHX1 overexpression promoted all three characteristics. In addition, results suggested EGR1 might partially mediate the effect of ZHX1 on the proliferation of CCA cells. Taken together, these results show ZHX1 promotes CCA cell proliferation, migration, and invasion, and present ZHX1 as a potential target for the treatment of CCA.
Department of Anatomy, School of Medicine, Pusan National University, Busandaehak-ro 49, Mulgeum-eup, Yangsan, 50612, Republic of Korea.|Gene & Therapy Research Center for Vessel-associated Diseases, Pusan National University, Busandaehak-ro 49, Mulgeum-eup, Yangsan, 50612, Republic of Korea.
Ryuk-Jun
RJ
Kwon
Department of Anatomy, School of Medicine, Pusan National University, Busandaehak-ro 49, Mulgeum-eup, Yangsan, 50612, Republic of Korea.|Gene & Therapy Research Center for Vessel-associated Diseases, Pusan National University, Busandaehak-ro 49, Mulgeum-eup, Yangsan, 50612, Republic of Korea.
Myoung-Eun
ME
Han
Department of Anatomy, School of Medicine, Pusan National University, Busandaehak-ro 49, Mulgeum-eup, Yangsan, 50612, Republic of Korea.|Gene & Therapy Research Center for Vessel-associated Diseases, Pusan National University, Busandaehak-ro 49, Mulgeum-eup, Yangsan, 50612, Republic of Korea.
Ji-Young
JY
Kim
Department of Anatomy, School of Medicine, Pusan National University, Busandaehak-ro 49, Mulgeum-eup, Yangsan, 50612, Republic of Korea.|Gene & Therapy Research Center for Vessel-associated Diseases, Pusan National University, Busandaehak-ro 49, Mulgeum-eup, Yangsan, 50612, Republic of Korea.
Liangwen
L
Liu
Department of Anatomy, School of Medicine, Pusan National University, Busandaehak-ro 49, Mulgeum-eup, Yangsan, 50612, Republic of Korea.|Gene & Therapy Research Center for Vessel-associated Diseases, Pusan National University, Busandaehak-ro 49, Mulgeum-eup, Yangsan, 50612, Republic of Korea.
Yun-Hak
YH
Kim
Department of Physiology, School of Medicine, Pusan National University, Busandaehak-ro 49, Mulgeum-eup, Yangsan, 50612, Republic of Korea.
Jin-Sup
JS
Jung
Department of Anatomy, School of Medicine, Pusan National University, Busandaehak-ro 49, Mulgeum-eup, Yangsan, 50612, Republic of Korea.|Gene & Therapy Research Center for Vessel-associated Diseases, Pusan National University, Busandaehak-ro 49, Mulgeum-eup, Yangsan, 50612, Republic of Korea.
Sae-Ock
SO
Oh
D001650:Bile Duct Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D001653:Bile Ducts, Intrahepatic / Q000378:metabolism / Q000473:pathology; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D018281:Cholangiocarcinoma / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D003094:Collagen / Q000737:chemistry; D015194:Diffusion Chambers, Culture; D004338:Drug Combinations; D051766:Early Growth Response Protein 1 / Q000235:genetics* / Q000378:metabolism; D015972:Gene Expression Regulation, Neoplastic; D018398:Homeodomain Proteins / Q000037:antagonists & inhibitors / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D007797:Laminin / Q000737:chemistry; D011509:Proteoglycans / Q000737:chemistry; D034741:RNA, Small Interfering / Q000235:genetics / Q000378:metabolism; D014157:Transcription Factors / Q000037:antagonists & inhibitors / Q000235:genetics* / Q000378:metabolism
D004338:Drug Combinations; C497325:EGR1 protein, human; D051766:Early Growth Response Protein 1; D018398:Homeodomain Proteins; D007797:Laminin; D011509:Proteoglycans; D034741:RNA, Small Interfering; D014157:Transcription Factors; C484404:ZHX1 protein, human; C059485:matrigel; D003094:Collagen