[Modalities for preparation, cryopreservation, thawing of hematopoietic stem cells and precautions for infusion to patient: Guidelines from the Francophone Society of Bone Marrow Transplantation and Cellular Therapy (SFGM-TC)].
To date, despite an existing regulatory framework and standards, there are no true technical recommendations. A survey of 23 cell processing facilities (France, Belgium and Switzerland) has allowed to overview current practices according to cellular products specifications upon arrival at the facility, with modalities for their preparation prior to cryopreservation, storage, thawing and finally for infusion to patient. Data analysis shows great variability of collected volumes and cell concentrations in cellular products. Despite homogeneous practices for handling cells at the facility, methods vary between centers, especially for the choice of cryoprotective solutions and thawing methods. During the workshop, practices have been discussed and summarized to write of recommendations about the following topics: processing and cryopreservation, thawing, bedside precautions (for infusion). This work identifies some improvements in terms of collection, choice of wash solution of thawed cells and validation of the conditions of carriage.
EFS Nord de France, UTC, 96, rue de Jemmapes, CS 22018, 59013 Lille cedex, France.
Florence
F
Boulanger
CHU Nancy, hôpitaux de Brabois, UTC BDT BSP, allée du Morvan, 54500 Vandoeuvre-Lès-Nancy, France.
Véronique
V
Decot
Grand hôpital de Charleroi (GHdC), laboratoire hématologie, 3, Grand'rue, 6000 Charleroi, Belgique.
Geneviève
G
Bulliard
Institut Paoli-Calmettes, centre de thérapie cellulaire, 232, boulevard Ste-Marguerite, BP 156, 13273 Marseille cedex 9, France.
Boris
B
Calmels
CHU-EFS Poitiers, banque de sang placentaire, 350, avenue Jacques-Cœur, 86012 Poitiers cedex, France.
Christine
C
Giraud
CHU Amiens Picardie, laboratoire de thérapie cellulaire, 80054 Amiens cedex 1, France.
Marie-Noëlle
MN
Lacassagne
Hôpital Necker-enfants malades, hématologie, 149, rue de Sèvres, 75015 Paris, France.
Alessandra
A
Magnani
EFS Bourgogne-Franche-Comte, ICT, BP 1937, 25020 Besançon cedex, France.
Fabienne
F
Pouthier
EFS Bretagne, TC et BSP, BP 91614, 35016 Rennes cedex, France.
Jean-Baptiste
JB
Thibert
Hôpitaux universitaires de Genève, hématologie, 4, rue Gabriel-Perret-Gentil, 1205 Genève, Suisse.
Yordanka
Y
Tirefort
CHU de Lille, université Lille 2, LIRIC Inserm U995, 59000 Lille, France.
Ibrahim
I
Yakoub-Agha
CHU Sart-Tilman, hématologie clinique, bâtiment B35, 4000 Liège, Belgique. Electronic address: e.baudoux@chu.ulg.ac.be.
Etienne
E
Baudoux
D001530:Belgium; D015925:Cryopreservation / Q000592:standards; D005602:France; D018380:Hematopoietic Stem Cell Transplantation / Q000379:methods*; D006412:Hematopoietic Stem Cells; D006801:Humans; D011292:Premedication / Q000379:methods / Q000592:standards; D058996:Quality Improvement; D012955:Societies, Medical; D011795:Surveys and Questionnaires; D013557:Switzerland
Cellules souches hématopoïétiques; Cryopreservation; Cryopréservation; Décongélation; Hematopoietic stem cells; Thawing