Preparing Plasmid Subclones for Capillary Sequencing.
This protocol describes the preparation of plasmid DNA from bacterial cultures or from archived cultures, based on the standard miniprep method. The resulting DNA is suitable in quantity and quality for use as template in capillary DNA sequencing. The protocol relies on the use of the Biomek automated liquid handling system.
Elaine
E
Mardis
W Richard
WR
McCombie
D001419:Bacteria / Q000235:genetics*; D004247:DNA / Q000737:chemistry* / Q000302:isolation & purification*; D010957:Plasmids / Q000302:isolation & purification*; D017422:Sequence Analysis, DNA / Q000379:methods*
D004247:DNA