AMPK in Yeast: The SNF1 (Sucrose Non-fermenting 1) Protein Kinase Complex.
In yeast, SNF1 protein kinase is the orthologue of mammalian AMPK complex. It is a trimeric complex composed of Snf1 protein kinase (orthologue of AMPKα catalytic subunit), Snf4 (orthologue of AMPKγ regulatory subunit), and a member of the Gal83/Sip1/Sip2 family of proteins (orthologues of AMPKβ subunit) that act as scaffolds and also regulate the subcellular localization of the complex. In this chapter, we review the recent literature on the characteristics of SNF1 complex subunits, the structure and regulation of the activity of the SNF1 complex, its role at the level of transcriptional regulation of relevant target genes and also at the level of posttranslational modification of targeted substrates. We also review the crosstalk of SNF1 complex activity with other key protein kinase pathways such as cAMP-PKA, TORC1, and PAS kinase.
Instituto de Biomedicina de Valencia, CSIC and Centro de Investigación Biomédica en Red de Enfermedades Raras (CIBERER-ISCiii), Jaime Roig 11, 46010, Valencia, Spain. sanz@ibv.csic.es.
Pascual
P
Sanz
Instituto de Biomedicina de Valencia, CSIC and Centro de Investigación Biomédica en Red de Enfermedades Raras (CIBERER-ISCiii), Jaime Roig 11, 46010, Valencia, Spain.
Rosa
R
Viana
Department of Biotecnología, Escuela Técnica Superior de Ingeniería Agronómica y del Medio Natural (ETSIAMN), Universitat Politécnica de Valencia, Valencia, Spain.
Maria Adelaida
MA
Garcia-Gimeno
D055372:AMP-Activated Protein Kinases / Q000235:genetics* / Q000378:metabolism; D042002:Chromatin Assembly and Disassembly; D015966:Gene Expression Regulation, Fungal; D005943:Gluconeogenesis; D006019:Glycolysis / Q000235:genetics; D050356:Lipid Metabolism; D055503:Protein Multimerization; D011499:Protein Processing, Post-Translational; D017346:Protein Serine-Threonine Kinases / Q000235:genetics* / Q000378:metabolism; D012097:Repressor Proteins / Q000235:genetics* / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics* / Q000378:metabolism; D015398:Signal Transduction; D013312:Stress, Physiological; D015534:Trans-Activators; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism
C085569:GAL83 protein, S cerevisiae; D012097:Repressor Proteins; C091267:SIP2 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; C561842:TORC1 protein complex, S cerevisiae; D015534:Trans-Activators; D014157:Transcription Factors; C071570:SNF1-related protein kinases; D017346:Protein Serine-Threonine Kinases; C075934:SIP1 protein, S cerevisiae; C078651:SNF4 protein, S cerevisiae; D055372:AMP-Activated Protein Kinases
Energy metabolism; Gal83; Glucose repression; Posttranslational regulation; Protein kinase; Protein structure analysis; Response to cellular stress; Sip1; Sip2; Snf1; Snf4; Transcriptional regulation