Genotoxic and anti-genotoxic effects of esculin and its oligomer fractions against mitomycin C-induced DNA damages in mice.
Mitomycin C is one of the most effective chemotherapeutic drugs against various solid tumors. However, despite its wide spectrum of clinical benefits, this agent is capable of inducing various types of genotoxicity. In this study, we investigated the effect of esculin and its oligomer fractions (E1, E2 and E3) against mitomycin C induced genotoxicity in liver and kidney cells isolated from Balb/C mice using the comet assay. Esculin and its oligomer fractions were not genotoxic at the tested doses (20 mg/kg and 40 mg/kg b.w). A significant decrease in DNA damages was observed, suggesting a protective role of esculin and its oligomer fractions against the genotoxicity induced by mitomycin C on liver and kidney cells. Moreover, esculin and its oligomer fractions did not induce an increase of malondialdehyde levels.
Laboratory of Cellular and Molecular Biology, Faculty of Dental Medicine, University of Monastir, Avicenna Street, 5000, Monastir, Tunisia; Unit of Bioactive and Natural Substances and Biotechnology UR12ES12, Faculty of Pharmacy, University of Monastir, Avicenna Street, 5000, Monastir, Tunisia.
Imen
I
Mokdad Bzeouich
Laboratory of Cellular and Molecular Biology, Faculty of Dental Medicine, University of Monastir, Avicenna Street, 5000, Monastir, Tunisia; Unit of Bioactive and Natural Substances and Biotechnology UR12ES12, Faculty of Pharmacy, University of Monastir, Avicenna Street, 5000, Monastir, Tunisia.
Nadia
N
Mustapha
Laboratory of Cellular and Molecular Biology, Faculty of Dental Medicine, University of Monastir, Avicenna Street, 5000, Monastir, Tunisia; Unit of Bioactive and Natural Substances and Biotechnology UR12ES12, Faculty of Pharmacy, University of Monastir, Avicenna Street, 5000, Monastir, Tunisia.
Mouna
M
Maatouk
Laboratory of Cellular and Molecular Biology, Faculty of Dental Medicine, University of Monastir, Avicenna Street, 5000, Monastir, Tunisia.
Kamel
K
Ghedira
Laboratory of Biomolecular Engineering, National School of Agronomy and Food Industries, National Polytechnics Institute of Lorraine ENSAIA-INPL, 54505, Vandoeuvre les Nancy, France.
Mohamed
M
Ghoul
Unit of Bioactive and Natural Substances and Biotechnology UR12ES12, Faculty of Pharmacy, University of Monastir, Avicenna Street, 5000, Monastir, Tunisia. Electronic address: leila.chekir@laposte.net.
Leila
L
Chekir-Ghedira
D000818:Animals; D000903:Antibiotics, Antineoplastic / Q000633:toxicity*; D016587:Antimutagenic Agents / Q000494:pharmacology* / Q000633:toxicity; D004249:DNA Damage / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D004929:Esculin / Q000494:pharmacology* / Q000633:toxicity; D007668:Kidney / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D015227:Lipid Peroxidation / Q000187:drug effects; D008099:Liver / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D008315:Malondialdehyde / Q000378:metabolism; D008807:Mice, Inbred BALB C; D048629:Micronuclei, Chromosome-Defective / Q000139:chemically induced / Q000187:drug effects*; D015162:Micronucleus Tests; D016685:Mitomycin / Q000633:toxicity*
D000903:Antibiotics, Antineoplastic; D016587:Antimutagenic Agents; D004929:Esculin; D008315:Malondialdehyde; D016685:Mitomycin
Antigenotoxicity; Esculin; Lipid peroxidation; Mitomycin C; Oligomer fractions