Association Between Type-specific HPV Infections and hTERT DNA Methylation in Patients with Invasive Cervical Cancer.
BACKGROUND
There exists limited information on the role of hTERT methylation, and its association with type-specific HPV infections in cervical cancer.


METHODS
Eighty-seven frozen samples were analyzed for type-specific HPV infection using a GP5+/GP6+PCR-RLB assay (RLB). hTERT DNA methylation analysis was performed using a newly developed PCR-RLB-hTERT.


RESULTS
Ninety-three percent of samples were HPV-positive and fifteen different types were detected. hTERT methylation analysis of region 1 revealed no methylation in 78.8% of the samples and partial methylation in 21.2%. In region two, 68.2% showed no methylation and 31.8% showed a pattern of partial methylation. An association between the alpha 9 and alpha 7 species with a pattern of no methylation of hTERT in the region 1 was established (p=0.02 and p=0.03, respectively).


CONCLUSIONS
Differences in patterns of methylation of the hTERT core promoter [region 1 (nt -208 to -1) and region 2 (nt +1 to +104) relative to first ATG] are related to the HPV species present.
Research Group in Cancer Biology, Research Branch, National Cancer Institute, Bogotá, Colombia.|Research Group in Radiobiology Clinical, Molecular and Cellular, National Cancer Institute, Bogotá, Colombia.|Microbiology and Infection Diseases, The Royal Women´s Hospital, Melbourne, VIC, Australia.
Mónica
M
Molano
Research Group in Cancer Biology, Research Branch, National Cancer Institute, Bogotá, Colombia pmoreno@cancer.gov.co.|Research Group in Radiobiology Clinical, Molecular and Cellular, National Cancer Institute, Bogotá, Colombia.
Pablo
P
Moreno-Acosta
Research Group in Cancer Biology, Research Branch, National Cancer Institute, Bogotá, Colombia.
Nicolás
N
Morales
Research Group in Cancer Biology, Research Branch, National Cancer Institute, Bogotá, Colombia.
Marcela
M
Burgos
Unit Group of Analysis, Research Branch, National Cancer Institute, Bogotá, Colombia.
Lina
L
Buitrago
Research Group in Radiobiology Clinical, Molecular and Cellular, National Cancer Institute, Bogotá, Colombia.|Unit Group of Analysis, Research Branch, National Cancer Institute, Bogotá, Colombia.
Oscar
O
Gamboa
Research Group in Cancer Biology, Research Branch, National Cancer Institute, Bogotá, Colombia.|Research Group in Radiobiology Clinical, Molecular and Cellular, National Cancer Institute, Bogotá, Colombia.
Rayner
R
Alvarez
Microbiology and Infection Diseases, The Royal Women´s Hospital, Melbourne, VIC, Australia.|Murdoch Children's Research Institute, Parkville, VIC, Australia.|Department of Obstetrics and Gynaecology, University of Melbourne, Parkville, VIC, Australia.
Suzanne M
SM
Garland
Microbiology and Infection Diseases, The Royal Women´s Hospital, Melbourne, VIC, Australia.|Murdoch Children's Research Institute, Parkville, VIC, Australia.|Department of Obstetrics and Gynaecology, University of Melbourne, Parkville, VIC, Australia.
Sepehr N
SN
Tabrizi
Department of Pathology, VU University Medical Center, Amsterdam, the Netherlands.
Renske D M
RD
Steenbergen
Oncological Pathology Group, National Cancer Institute, Bogotá, Colombia.
Juan Carlos
JC
Mejía
D019175:DNA Methylation / Q000235:genetics*; D005260:Female; D006367:HeLa Cells; D052162:Human papillomavirus 16 / Q000235:genetics / Q000472:pathogenicity; D052161:Human papillomavirus 18 / Q000235:genetics / Q000472:pathogenicity; D006801:Humans; D009361:Neoplasm Invasiveness / Q000235:genetics; D030361:Papillomavirus Infections / Q000145:classification / Q000235:genetics* / Q000821:virology; D019098:Telomerase / Q000235:genetics*; D002583:Uterine Cervical Neoplasms / Q000235:genetics* / Q000473:pathology / Q000821:virology
C509186:TERT protein, human; D019098:Telomerase
DNA methylation; papillomavirus infections; telomerase reverse transcriptase; uterine cervical neoplasms