Aurora B kinase is required for cell cycle progression in silkworm.
Aurora B kinase, a member of serine/threonine kinase family, is the catalytic subunit of the chromosomal passenger complex and is essential for chromosome alignment, chromosome segregation, and cytokinesis during mitosis. Here, we cloned the full-length cDNA sequence of silkworm Aurora B (BmAurB) gene and predicted that BmAurB protein contains a conserved S_TKc domain. Phylogenetic analysis between BmAurB and other Aurora kinases indicates that Aurora kinases may have evolved after separation between mammalian and insect, and prior to radiation of either mammalian or insects. RT-PCR examination revealed that the expression of the BmAurB gene was high in mitotic cycling gonads, moderate in mitotic cycling brain, and undetectable in endocycling silk gland during silkworm larval development. RNAi or inhibitor-mediated inhibition of the BmAurB gene in silkworm ovary-derived BmN4-SID1 cells disrupted cell cycle progression during mitosis and induced an accumulation of polyploid cells, cell cycle arrest at G2/M phase, chromosome misalignment, chromosome bridge, and bi-nucleation. Taken together, our results suggest that the BmAurB gene is required for cell cycle progression in silkworm.
State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400715, China.
Xiaoxu
X
Gang
State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400715, China.
Wenliang
W
Qian
State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400715, China.
Tianlei
T
Zhang
State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400715, China.
Xinxin
X
Yang
State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400715, China.
Qingyou
Q
Xia
State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400715, China. Electronic address: chengdj@swu.edu.cn.
Daojun
D
Cheng
D000595:Amino Acid Sequence; D000818:Animals; D064107:Aurora Kinase B / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D001483:Base Sequence; D012831:Bombyx / Q000166:cytology* / Q000201:enzymology* / Q000235:genetics; D059447:Cell Cycle Checkpoints / Q000235:genetics; D002460:Cell Line; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D019143:Evolution, Molecular; D005260:Female; D015971:Gene Expression Regulation, Enzymologic; D017344:Genes, Insect; D019476:Insect Proteins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D010053:Ovary / Q000166:cytology / Q000201:enzymology; D010802:Phylogeny; D034622:RNA Interference
D018076:DNA, Complementary; D019476:Insect Proteins; D064107:Aurora Kinase B
Aurora B kinase; Inhibitor; Mitosis; RNAi; Silkworm