MAS1H NMR Probes Freezing Point Depression of Water and Liquid-Gel Phase Transitions in Liposomes.
The lipid bilayer typical of hydrated biological membranes is characterized by a liquid-crystalline, highly dynamic state. Upon cooling or dehydration, these membranes undergo a cooperative transition to a rigidified, more-ordered, gel phase. This characteristic phase transition is of significant biological and biophysical interest, for instance in studies of freezing-tolerant organisms. Magic-angle-spinning (MAS) solid-state NMR (ssNMR) spectroscopy allows for the detection and characterization of the phase transitions over a wide temperature range. In this study we employ MAS1H NMR to probe the phase transitions of both solvent molecules and different hydrated phospholipids, including tetraoleoyl cardiolipin (TOCL) and several phosphatidylcholine lipid species. The employed MAS NMR sample conditions cause a previously noted substantial reduction in the freezing point of the solvent phase. The effect on the solvent is caused by confinement of the aqueous solvent in the small and densely packed MAS NMR samples. In this study we report and examine how the freezing point depression also impacts the lipid phase transition, causing a ssNMR-observed reduction in the lipids' melting temperature (Tm). The molecular underpinnings of this phenomenon are discussed and compared with previous studies of membrane-associated water phases and the impact of membrane-protective cryoprotectants.
Department of Structural Biology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania.
Abhishek
A
Mandal
Department of Structural Biology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania. Electronic address: vanderwel@pitt.edu.
Patrick C A
PCA
van der Wel
D005615:Freezing; D005782:Gels; D008051:Lipid Bilayers / Q000737:chemistry; D008081:Liposomes / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D012997:Solvents / Q000737:chemistry; D013696:Temperature; D014867:Water / Q000737:chemistry*
D005782:Gels; D008051:Lipid Bilayers; D008081:Liposomes; D012997:Solvents; D014867:Water