Systems biology for understanding and engineering of heterotrophic oleaginous microorganisms.
Heterotrophic oleaginous microorganisms continue to draw interest as they can accumulate a large amount of lipids which is a promising feedstock for the production of biofuels and oleochemicals. Nutrient limitation, especially nitrogen limitation, is known to effectively trigger the lipid production in these microorganisms. For the aim of developing improved strains, the mechanisms behind the lipid production have been studied for a long time. Nowadays, system-level understanding of their metabolism and associated metabolic switches is attainable with modern systems biology tools. This work reviews the systems biology studies, based on (i) top-down, large-scale 'omics' tools, and (ii) bottom-up, mathematical modeling methods, on the heterotrophic oleaginous microorganisms with an emphasis on further application to metabolic engineering.
School of Chemical and Biological Engineering, Institute of Molecular Biology and Genetics, and Bioengineering Institute, Seoul National University, Seoul, Republic of Korea.
Beom Gi
BG
Park
School of Chemical and Biological Engineering, Institute of Molecular Biology and Genetics, and Bioengineering Institute, Seoul National University, Seoul, Republic of Korea.
Minsuk
M
Kim
School of Chemical and Biological Engineering, Institute of Molecular Biology and Genetics, and Bioengineering Institute, Seoul National University, Seoul, Republic of Korea.
Joonwon
J
Kim
Interdisciplinary Program for Biochemical Engineering and Biotechnology, Seoul National University, Seoul, Republic of Korea.
Heewang
H
Yoo
School of Chemical and Biological Engineering, Institute of Molecular Biology and Genetics, and Bioengineering Institute, Seoul National University, Seoul, Republic of Korea.|Interdisciplinary Program for Biochemical Engineering and Biotechnology, Seoul National University, Seoul, Republic of Korea.
Byung-Gee
BG
Kim
D001419:Bacteria / Q000235:genetics / Q000378:metabolism; D005658:Fungi / Q000235:genetics / Q000378:metabolism; D052836:Heterotrophic Processes; D050356:Lipid Metabolism; D060847:Metabolic Engineering / Q000379:methods*; D008828:Microbiological Techniques; D008962:Models, Theoretical; D049490:Systems Biology / Q000379:methods*
Constraint-based modeling; Metabolic switch; Oleaginous microorganisms; Omics; Systems biology