In VitroAnti-AChE, Anti-BuChE, and Antioxidant Activity of 12 Extracts ofEleutherococcusSpecies.
Neurodegenerative diseases are one of the most occurring diseases in developed and developing countries. The aim of this work focused on the screening of the natural inhibitors of AChE and BuChE and antioxidants inEleutherococcusspecies. We found that the ethanol extracts ofE. setchuenensisandE. sessiliflorusshowed the strongest inhibition towards AChE (IC50: 0.3 and 0.3 mg/mL, resp.). Among chloroform extracts, the most active appeared to beE. gracilistylus(IC50: 0.37 mg/mL). In turn, the ethanol extract ofE. henryiinhibited the strongest BuChE with IC50value of 0.13 mg/mL. Among chloroform extracts,E. gracilistylus,E. setchuenensis, andE. sessiliflorusappeared to be the strongest with IC50values of 0.12, 0.18, and 0.19 mg/mL. HPTLC screening confirmed the presence of inhibitors in extracts. All extracts exhibited anti-DPPH⁎activity and single antioxidants have been identified. To the best of our knowledge, no information was available on this activity of compounds inEleutherococcus. These studies provide a biochemical basis for the regulation of AChE and BuChE and encourage us to continue isolation of active compounds.
Department of Pharmacognosy, Collegium Medicum, Jagiellonian University, 9 Medyczna Street, 30-688 Cracow, Poland.
Daniel
0000-0002-7821-7002
D
Załuski
Department of Pharmacognosy, Ludwik Rydygier Collegium Medicum, Nicolaus Copernicus University, 9 Marie Curie-Skłodowska Street, 85-094 Bydgoszcz, Poland.
Rafał
0000-0002-8250-707X
R
Kuźniewski
D000110:Acetylcholinesterase / Q000378:metabolism*; D000975:Antioxidants / Q000494:pharmacology*; D002091:Butyrylcholinesterase / Q000378:metabolism*; D002725:Chloroform; D002800:Cholinesterase Inhibitors / Q000494:pharmacology*; D002855:Chromatography, Thin Layer; D028001:Eleutherococcus / Q000737:chemistry*; D000431:Ethanol; D020128:Inhibitory Concentration 50; D010936:Plant Extracts / Q000494:pharmacology*
D000975:Antioxidants; D002800:Cholinesterase Inhibitors; D010936:Plant Extracts; D000431:Ethanol; D002725:Chloroform; D000110:Acetylcholinesterase; D002091:Butyrylcholinesterase