Anti-inflammatory effect of supercritical extract and its constituents from Ishige okamurae.
The anti-inflammatory properties of the supercritical fluid extract ofIshige okamurae(SFEIO) on lipopolysaccharide (LPS)-stimulated murine RAW 264.7 macrophages. The lipid profile of the SFEIO, reviled the presence of palmitic acid (220.2 mg/g), linoleic acid (168.0 mg/g), and oleic acid (123.0 mg/g). SFEIO was found to exert it's anti-inflammatory effects through inhibiting nitric oxide (NO), prostaglandin E2(PGE2), inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), tumor necrosis factor (TNF)-α, interleukin (IL)-1β, and IL-6 production in LPS-stimulated RAW 264.7 cells, without inducing cytotoxicity. SFEIO did not effect on the LPS-induced p38 kinase phosphorylation, whereas it attenuated the extracellular-related signaling kinase (ERK) and c-Jun N-terminal kinase (JNK) phosphorylation. Furthermore, SFEIO inhibited the LPS-induced IκB-α degradation and p50 NF-κB activation. These results suggest that SFEIO exerts its anti-inflammatory effects in LPS-activated RAW 264.7 cells by down-regulating the activation of ERK, JNK, and NF-κB.
Jeju Center, Korea Basic Science Institute (KBSI), Jeju 690-140, Republic of Korea; Department of Marine Life Science, Jeju National University, Jeju 690-756, Republic of Korea.
Eun-Yi
EY
Ko
Jeju Biodiversity Research Institute, Jeju Technopark, Jeju, 699-943, Republic of Korea.
Weon-Jong
WJ
Yoon
World Institute of Kimchi, Gwangju 503-360, Republic of Korea.
Hae-Won
HW
Lee
Jeju International Marine Science Center for Research & Education, Korea Institute of Ocean Science & Technology (KIOST), Jeju, 63349, Republic of Korea.
Soo-Jin
SJ
Heo
Department of Food Bioengineering, Jeju National University, Jeju 690-756, Republic of Korea.
Young-Hwan
YH
Ko
Department of Marine Life Science, Jeju National University, Jeju 690-756, Republic of Korea.
I P Shanura
IP
Fernando
Jeju Center, Korea Basic Science Institute (KBSI), Jeju 690-140, Republic of Korea.
Kichul
K
Cho
Jeju Center, Korea Basic Science Institute (KBSI), Jeju 690-140, Republic of Korea; Department of Marine Life Science, Jeju National University, Jeju 690-756, Republic of Korea.
Chi-Heon
CH
Lee
Jeju International Marine Science Center for Research & Education, Korea Institute of Ocean Science & Technology (KIOST), Jeju, 63349, Republic of Korea.
Sung-Pyo
SP
Hur
Jeju Center, Korea Basic Science Institute (KBSI), Jeju 690-140, Republic of Korea; Department of Marine Life Science, Jeju National University, Jeju 690-756, Republic of Korea.
Su-Hyeon
SH
Cho
Department of Marine Bio-Food Sciences, Chonnam National University, Yeosu 550-74, Republic Korea.
Ginnae
G
Ahn
Jeju Center, Korea Basic Science Institute (KBSI), Jeju 690-140, Republic of Korea.
Daekyung
D
Kim
Jeju Center, Korea Basic Science Institute (KBSI), Jeju 690-140, Republic of Korea; Department of Marine Biotechnology, University of Science and Technology, Daejeon 305-350, Republic of Korea.
Kil-Nam
KN
Kim
Ishige okamurae; NF-kappaB; anti-inflammatory; linoleic acid; supercritical fluid extraction