Characterization of complete mitochondrial genome of fives tripe wrasse (Thalassoma quinquevittatum, Lay & Bennett, 1839) and phylogenetic analysis.
To further supplement the genome-level features in related species, T. quinquevittatum complete mtDNA was firstly sequenced and de novo assembled by next-generation sequencing. The full-length mtDNA of T. quinquevittatum was a 16,896bp fragment, which was atypical of Labridae, with 2 ribosomal RNA (rRNA) genes, 13 protein-coding genes (PCGs), 23 transfer RNA (tRNA) genes, and a major non-coding control region (D-loop region). Additionally, the mtDNA of T. quinquevittatum exhibited characteristics of A (27.1%), T (29.3%), G (17.8%), and C (25.8%) with a high A+T content (56.4%). Furthermore, the analysis of the average Ka/Ks in the 13 PCGs of three Labridae species indicated a strong purifying selection within this group. Additionally, the phylogenetic analysis based on 13 concatenated PCGs nucleotide and amino acid datasets, showed high value support for the following sister clade among the four genera (T. quinquevittatum, Halichoeres trimaculatus, Halichoeres melanurus, Parajulis poecilepterus). The complete mtDNA of the T. quinquevittatum provided important information for the study in population genetics and evolutionary theory.
Key Laboratory of South China Sea Fishery Resources Exploitation & Utilization, Ministry of Agriculture, Key Laboratory of Fishery Ecology & Environment, Guangdong Province, Division of Aquaculture and Genetic Breeding, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, 231 Xingang Road West, Haizhu District, Guangzhou 510300, China.
Kecheng
K
Zhu
Key Laboratory of South China Sea Fishery Resources Exploitation & Utilization, Ministry of Agriculture, Key Laboratory of Fishery Ecology & Environment, Guangdong Province, Division of Aquaculture and Genetic Breeding, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, 231 Xingang Road West, Haizhu District, Guangzhou 510300, China.
Na
N
Wu
Key Laboratory of South China Sea Fishery Resources Exploitation & Utilization, Ministry of Agriculture, Key Laboratory of Fishery Ecology & Environment, Guangdong Province, Division of Aquaculture and Genetic Breeding, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, 231 Xingang Road West, Haizhu District, Guangzhou 510300, China.
Xiaoxiao
X
Sun
Key Laboratory of South China Sea Fishery Resources Exploitation & Utilization, Ministry of Agriculture, Key Laboratory of Fishery Ecology & Environment, Guangdong Province, Division of Aquaculture and Genetic Breeding, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, 231 Xingang Road West, Haizhu District, Guangzhou 510300, China.
Huayang
H
Guo
Key Laboratory of South China Sea Fishery Resources Exploitation & Utilization, Ministry of Agriculture, Key Laboratory of Fishery Ecology & Environment, Guangdong Province, Division of Aquaculture and Genetic Breeding, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, 231 Xingang Road West, Haizhu District, Guangzhou 510300, China.
Nan
N
Zhang
Key Laboratory of South China Sea Fishery Resources Exploitation & Utilization, Ministry of Agriculture, Key Laboratory of Fishery Ecology & Environment, Guangdong Province, Division of Aquaculture and Genetic Breeding, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, 231 Xingang Road West, Haizhu District, Guangzhou 510300, China; South China Sea Bio-Resource Exploitation and Utilization Collaborative Innovation Center, China.
Shigui
S
Jiang
Key Laboratory of South China Sea Fishery Resources Exploitation & Utilization, Ministry of Agriculture, Key Laboratory of Fishery Ecology & Environment, Guangdong Province, Division of Aquaculture and Genetic Breeding, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, 231 Xingang Road West, Haizhu District, Guangzhou 510300, China. Electronic address: zhangdch@scsfri.ac.cn.
Dianchang
D
Zhang
D000818:Animals; D001482:Base Composition; D001483:Base Sequence; D003062:Codon; D004272:DNA, Mitochondrial / Q000235:genetics; D019143:Evolution, Molecular; D029941:Fish Proteins / Q000235:genetics; D005828:Genetics, Population; D054629:Genome, Mitochondrial; D024101:Mitochondrial Proteins / Q000235:genetics; D009690:Nucleic Acid Conformation; D010473:Perciformes / Q000145:classification / Q000235:genetics*; D010802:Phylogeny; D012335:RNA, Ribosomal / Q000235:genetics; D012343:RNA, Transfer / Q000737:chemistry / Q000235:genetics; D012641:Selection, Genetic
D003062:Codon; D004272:DNA, Mitochondrial; D029941:Fish Proteins; D024101:Mitochondrial Proteins; D012335:RNA, Ribosomal; D012343:RNA, Transfer
Ka/Ks; Mitochondrial DNA; Phylogenetic analysis; Thalassoma quinquevittatum