Facile synthesis of Prussian blue nanocubes/silver nanowires network as a water-based ink for the direct screen-printed flexible biosensor chips.
The large-scale fabrication of nanocomposite based biosensors is always a challenge in the technology commercialization from laboratory to industry. In order to address this issue, we have designed a facile chemical method of fabricated nanocomposite ink applied to the screen-printed biosensor chip. This ink can be derived in the water through the in-situ growth of Prussian blue nanocubes (PBNCs) on the silver nanowires (AgNWs) to construct a composite nanostructure by a facile chemical method. Then a miniature flexible biosensor chip was screen-printed by using the prepared nanocomposite ink. Due to the synergic effects of the large specific surface area, high conductivity and electrocatalytic activity from AgNWs and PBNCs, the as-prepared biosensor chip exhibited a fast response (<3s)， a wider linear response from 0.01 to 1.3mM with an ultralow LOD=5µm, and the ultrahigh sensitivities of 131.31 and 481.20µAmM-1cm-2for the detections of glucose and hydrogen peroxide (H2O2), respectively. Furthermore, the biosensor chip exhibited excellent stability, good reproducibility and high anti-interference ability towards physiological substances under a very low working potential of -0.05. Hence, the proposed biosensor chip also showed a promising potential for the application in practical analysis.
State Key Laboratory of Materials-Oriented Chemical Engineering, College of Chemical Engineering, Nanjing Tech University, Nanjing 210009, PR China.
Pengqi
P
Yang
State Key Laboratory of Materials-Oriented Chemical Engineering, College of Chemical Engineering, Nanjing Tech University, Nanjing 210009, PR China.
Jingmeng
J
Peng
State Key Laboratory of Materials-Oriented Chemical Engineering, College of Chemical Engineering, Nanjing Tech University, Nanjing 210009, PR China.
Zhenyu
Z
Chu
State Key Laboratory of Materials-Oriented Chemical Engineering, College of Chemical Engineering, Nanjing Tech University, Nanjing 210009, PR China.
Danfeng
D
Jiang
State Key Laboratory of Materials-Oriented Chemical Engineering, College of Chemical Engineering, Nanjing Tech University, Nanjing 210009, PR China. Electronic address: wqjin@njtech.edu.cn.
Wanqin
W
Jin
D000818:Animals; D001234:Aspergillus niger / Q000201:enzymology; D015374:Biosensing Techniques / Q000295:instrumentation*; D001786:Blood Glucose / Q000032:analysis*; D004566:Electrodes; D004800:Enzymes, Immobilized / Q000737:chemistry; D005295:Ferrocyanides / Q000737:chemistry*; D005949:Glucose Oxidase / Q000737:chemistry; D006861:Hydrogen Peroxide / Q000032:analysis*; D007281:Ink; D056656:Lab-On-A-Chip Devices; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure; D053770:Nanowires / Q000737:chemistry / Q000648:ultrastructure; D011817:Rabbits; D015203:Reproducibility of Results; D012834:Silver / Q000737:chemistry*; D014867:Water / Q000737:chemistry
D001786:Blood Glucose; D004800:Enzymes, Immobilized; D005295:Ferrocyanides; D014867:Water; D012834:Silver; D006861:Hydrogen Peroxide; D005949:Glucose Oxidase; C000170:ferric ferrocyanide
High sensitivity; Prussian blue nanocubes; Screen-printed biosensor chips; Silver nanowires