Beta-adrenergic signaling affect osteoclastogenesis via osteocytic MLO-Y4 cells' RANKL production.
The sympathetic nervous system play a pivotal role in bone remodeling through β-adrenoceptor (β-AR). However, it is not well documented whether the β-adrenoceptor pathway has the potential to influence osteocytes. In this study, cell viability, the expression of β-AR subtypes, enzymes of catecholamine synthesis or degradation, bone-related gene and protein in osteocytic MLO-Y4 cells were investigated by β-adrenergic stimulation. Isoproterenol (ISO) promoted RANKL to OPG expression in osteocytes, as well as osteoclasts formation in osteocytes-RAW264.7 cell co-cultures but not RAW264.7 cell monoculture. The ISO-stimulated effect was enhanced in β1-AR antagonist pretreatment, but was rescued by blocking β2-AR. The results indicate that β1-and β2-AR play reciprocal roles on MLO-Y4 cells in the regulation of osteoclastogenesis, and osteocyte β-adrenergic signaling might be a new valuable therapy for bone disease.
Oral Medical Center, The Second Xiangya Hospital, Central South University, Changsha, China.
Qianqian
Q
Yao
Department of Thoracic Surgery, The Second Xiangya Hospital, Central South University, Changsha, China.
Hengxing
H
Liang
Dental Implant Center, West China Hospital of Stomatology, Sichuan University, Chengdu, China; State Key Laboratory of Oral Disease, West China Hospital of Stomatology, Sichuan University, Chengdu, China.
Bo
B
Huang
Dental Implant Center, West China Hospital of Stomatology, Sichuan University, Chengdu, China; State Key Laboratory of Oral Disease, West China Hospital of Stomatology, Sichuan University, Chengdu, China.
Lin
L
Xiang
Dental Implant Center, West China Hospital of Stomatology, Sichuan University, Chengdu, China; State Key Laboratory of Oral Disease, West China Hospital of Stomatology, Sichuan University, Chengdu, China.
Tianlu
T
Wang
Dental Implant Center, West China Hospital of Stomatology, Sichuan University, Chengdu, China; State Key Laboratory of Oral Disease, West China Hospital of Stomatology, Sichuan University, Chengdu, China.
Yi
Y
Xiong
Dental Implant Center, West China Hospital of Stomatology, Sichuan University, Chengdu, China; State Key Laboratory of Oral Disease, West China Hospital of Stomatology, Sichuan University, Chengdu, China.
Bo
B
Yang
Dental Implant Center, West China Hospital of Stomatology, Sichuan University, Chengdu, China; State Key Laboratory of Oral Disease, West China Hospital of Stomatology, Sichuan University, Chengdu, China.
Yanjun
Y
Guo
Dental Implant Center, West China Hospital of Stomatology, Sichuan University, Chengdu, China; State Key Laboratory of Oral Disease, West China Hospital of Stomatology, Sichuan University, Chengdu, China. Electronic address: dentistgong@hotmail.com.
Ping
P
Gong
D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D007545:Isoproterenol / Q000494:pharmacology; D051379:Mice; D010011:Osteocytes / Q000187:drug effects / Q000378:metabolism*; D010012:Osteogenesis; D053245:RANK Ligand / Q000096:biosynthesis*; D000067996:RAW 264.7 Cells; D011943:Receptors, Adrenergic, beta / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction
D053245:RANK Ligand; D011943:Receptors, Adrenergic, beta; D007545:Isoproterenol
Osteoclastogenesis; Osteocytes; Sympathetic nervous system; β-adrenoceptor