A sensitive electrochemiluminescence immunosensor based on Ru(bpy)32+in 3D CuNi oxalate as luminophores and graphene oxide-polyethylenimine as released Ru(bpy)32+initiator.
In this work, electrochemiluminescence (ECL) luminophor Ru(bpy)32+was encapsulated in 3D CuNi oxalate and the synthesized metal-inorganic frameworks {[Ru(bpy)3][Cu2xNi2(1-x)(ox)3]}n(Ru/Cu/Ni) exhibited excellent and stable ECL signals, which could be decreased by polyethylenimine capped graphene oxide (GO-PEI). Based on this, a new sandwich ECL immunosensor was developed for detection of carcinoembryonic antigen (CEA). To capture primary antibody and second antibody more simply and efficiently, Ag nanoparticles were doped with Ru/Cu/Ni and GO-PEI. After a sandwich-type immunoreaction, a remarkable decrease of ECL signal was observed due to the release of Ru(bpy)32+which was caused by the coordination between PEI and metal ions. Under the optimization of determination conditions, a linear response range for CEA from 0.1pgmL-1to 100ngmL-1was obtained, and the detection limit was calculated to be 0.027pgmL-1(S/N=3). The prepared CEA immunosensor displayed high sensitivity, excellent stability and good specificity.
Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, PR China.
Xiaojian
X
Li
Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, PR China.
Siqi
S
Yu
Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, PR China.
Tao
T
Yan
Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, PR China.
Yong
Y
Zhang
Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, PR China.
Bin
B
Du
Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, PR China. Electronic address: wudan791108@163.com.
Dan
D
Wu
Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, PR China.
Qin
Q
Wei
D015374:Biosensing Techniques; D002272:Carcinoembryonic Antigen / Q000737:chemistry / Q000302:isolation & purification*; D003300:Copper / Q000737:chemistry; D055664:Electrochemical Techniques; D006108:Graphite / Q000737:chemistry; D006801:Humans; D007118:Immunoassay; D057230:Limit of Detection; D053768:Metal Nanoparticles / Q000737:chemistry; D009532:Nickel / Q000737:chemistry; D011094:Polyethyleneimine / Q000737:chemistry; D012822:Silicon Dioxide / Q000737:chemistry
D002272:Carcinoembryonic Antigen; D012822:Silicon Dioxide; D006108:Graphite; D003300:Copper; D009532:Nickel; D011094:Polyethyleneimine
Ag nanoparticles; Coordination; CuNi oxalate; Polyethylenimine capped graphene oxide