Zwitterionic peptide anchored to conducting polymer PEDOT for the development of antifouling and ultrasensitive electrochemical DNA sensor.
Zwitterionic peptides were anchored to a conducting polymer of citrate doped poly(3,4-ethylenedioxythiophene) (PEDOT) via the nickel cation coordination, and the obtained peptide modified PEDOT, with excellent antifouling ability and good conductivity, was further used for the immobilization of a DNA probe to construct an electrochemical biosensor for the breast cancer marker BRCA1. The DNA biosensor was highly sensitive (with detection limit of 0.03fM) and selective, and it was able to detect BRCA1 in 5% (v/v) human plasma with satisfying accuracy and low fouling. The marriage of antifouling and biocompatible peptides with conducting polymers opened a new avenue to construct electrochemical biosensors capable of assaying targets in complex biological media with high sensitivity and without biofouling.
Key Laboratory of Sensor Analysis of Tumor Marker, Ministry of Education, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, China; College of Chemistry and Chemical Engineering, Taishan University, Taian 271021, PR China.
Guixiang
G
Wang
Key Laboratory of Sensor Analysis of Tumor Marker, Ministry of Education, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, China.
Rui
R
Han
Key Laboratory of Sensor Analysis of Tumor Marker, Ministry of Education, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, China.
Xiaoli
X
Su
Key Laboratory of Sensor Analysis of Tumor Marker, Ministry of Education, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, China.
Yinan
Y
Li
Key Laboratory of Sensor Analysis of Tumor Marker, Ministry of Education, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, China.
Guiyun
G
Xu
Key Laboratory of Sensor Analysis of Tumor Marker, Ministry of Education, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, China. Electronic address: xiliangluo@qust.edu.cn.
Xiliang
X
Luo
D019313:BRCA1 Protein / Q000235:genetics*; D015374:Biosensing Techniques / Q000379:methods*; D019086:Bridged Bicyclo Compounds, Heterocyclic / Q000737:chemistry*; D004247:DNA / Q000032:analysis / Q000097:blood* / Q000235:genetics*; D015342:DNA Probes / Q000737:chemistry / Q000235:genetics; D055664:Electrochemical Techniques / Q000379:methods; D006801:Humans; D061925:Immobilized Nucleic Acids / Q000737:chemistry / Q000235:genetics; D057230:Limit of Detection; D010455:Peptides / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry*
D019313:BRCA1 Protein; D019086:Bridged Bicyclo Compounds, Heterocyclic; D015342:DNA Probes; D061925:Immobilized Nucleic Acids; D010455:Peptides; D011108:Polymers; C121383:poly(3,4-ethylene dioxythiophene); D004247:DNA
Antifouling biosensor; Cancer biomarker; DNA; Electrochemistry; PEDOT; Peptide