Genome-Wide Identification and Transferability of Microsatellite Markers between Palmae Species.
The Palmae family contains 202 genera and approximately 2800 species. Except forElaeis guineensisandPhoenix dactylifera, almost no genetic and genomic information is available for Palmae species. Therefore, this is an obstacle to the conservation and genetic assessment of Palmae species, especially those that are currently endangered. The study was performed to develop a large number of microsatellite markers which can be used for genetic analysis in different Palmae species. Based on the assembled genome ofE. guineensisandP. dactylifera, a total of 814 383 and 371 629 microsatellites were identified. Among these microsatellites identified inE. guineensis, 734 509 primer pairs could be designed from the flanking sequences of these microsatellites. The majority (618 762) of these designed primer pairs hadin silicoproducts in the genome ofE. guineensis. These 618 762 primer pairs were subsequently used toin silicoamplify the genome ofP. dactylifera. A total of 7 265 conserved microsatellites were identified betweenE. guineensisandP. dactylifera. One hundred and thirty-five primer pairs flanking the conserved SSRs were stochastically selected and validated to have high cross-genera transferability, varying from 16.7 to 93.3% with an average of 73.7%. These genome-wide conserved microsatellite markers will provide a useful tool for genetic assessment and conservation of different Palmae species in the future.
Hainan Key Laboratory of Tropical Oil Crops Biology/Coconut Research Institute, Chinese Academy of Tropical Agricultural Sciences Wenchang, China.
Yong
Y
Xiao
Hainan Key Laboratory of Tropical Oil Crops Biology/Coconut Research Institute, Chinese Academy of Tropical Agricultural SciencesWenchang, China; College of Agriculture, Hainan UniversityHaikou, China.
Wei
W
Xia
Hainan Key Laboratory of Tropical Oil Crops Biology/Coconut Research Institute, Chinese Academy of Tropical Agricultural Sciences Wenchang, China.
Jianwei
J
Ma
Department of Plant Breeding, IFZ Research Centre for Biosystems, Land Use and Nutrition, Justus Liebig University Giessen Giessen, Germany.
Annaliese S
AS
Mason
Hainan Key Laboratory of Tropical Oil Crops Biology/Coconut Research Institute, Chinese Academy of Tropical Agricultural Sciences Wenchang, China.
Haikuo
H
Fan
Hainan Key Laboratory of Tropical Oil Crops Biology/Coconut Research Institute, Chinese Academy of Tropical Agricultural Sciences Wenchang, China.
Peng
P
Shi
Hainan Key Laboratory of Tropical Oil Crops Biology/Coconut Research Institute, Chinese Academy of Tropical Agricultural Sciences Wenchang, China.
Xintao
X
Lei
Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Science Haikou, China.
Zilong
Z
Ma
Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Science Haikou, China.
Ming
M
Peng
Elaeis guineensis; Phoenix dactylifera; cross-genera transferability; microsatellite; palm