Optimized medium culture for Acidobacteria subdivision 1 strains.
Members of subdivision 1 of the phylum Acidobacteria were grown at different pH values in a new medium formulation named PSYL 5, which includes sucrose as a carbon source and other compounds (such as KH2PO4 and MgSO4.7H2O). Growth rate was nearly constant at pH 5.0 and declined at pH 3-4 and 6-7. However, it was found that effects involving good carbon/nitrogen ratios and pH on the growth of the members of Acidobacteria subdivision 1 were significant, and the strongest effect of these conditions was at pH 5.0. In addition, incubation time of 48, 72, 96 and 120 h was shorter than that described previously for members of Acidobacteria subdivision 1 on solid laboratory media.
Departamento de Tecnologia, UNESP-Universidade Estadual Paulista, Faculdade de Ciências Agrárias e Veterinárias, Rod. Prof. Paulo Donato Castellane km 5, CEP 14884-900 Jaboticabal, SP, Brazil.
João Carlos
JC
Campanharo
Netherlands Institute of Ecology (NIOO-KNAW), Department of Microbial Ecology, PO Box 50, 6700 AB, Wageningen, the Netherlands.
Anna Maria
AM
Kielak
Departamento de Tecnologia, UNESP-Universidade Estadual Paulista, Faculdade de Ciências Agrárias e Veterinárias, Rod. Prof. Paulo Donato Castellane km 5, CEP 14884-900 Jaboticabal, SP, Brazil.
Tereza Cristina Luque
TCL
Castellane
Netherlands Institute of Ecology (NIOO-KNAW), Department of Microbial Ecology, PO Box 50, 6700 AB, Wageningen, the Netherlands.
Eiko Eurya
EE
Kuramae
Departamento de Tecnologia, UNESP-Universidade Estadual Paulista, Faculdade de Ciências Agrárias e Veterinárias, Rod. Prof. Paulo Donato Castellane km 5, CEP 14884-900 Jaboticabal, SP, Brazil.
Eliana Gertrudes de Macedo
EGM
Lemos

          Acidobacteria
        ; 
          Acidobacteria subdivision 1; colony growth; growth in sucrose