Rotating packed bed reactor for enzymatic synthesis of biodiesel.
The aim of the present work was to study the applicability of rotating packed bed (RPB) for biodiesel through the biocatalytic method. In this research, the RPB facilitated a more homogeneous mixture of substrates due to its higher mass transfer efficiency and better micromixing environment. This was superior to the traditional continuous stirred tank reactor (CSTR) system. Candida sp. 99-125 lipase was used without any organic solvent or additive, and demonstrated a significant catalyst efficiency. The key factors, such as the high gravity factor (β), pattern of the catalyst and methanol-FFA molar ratio etc. were investigated. Under the optimal conditions, the hydrolysis yield of fatty acids was 97.0% after 24h and the esterification yield of biodiesel was 96.0% 6h later. The esterifying yield didn't have an obvious decline in the fifth batch. Consequently, the RPB is an attractive and effective reactor for enzymatic synthesis.
Beijing Bioprocess Key Laboratory, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, PR China.
Juntao
J
Xu
Beijing Bioprocess Key Laboratory, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, PR China.
Changsheng
C
Liu
Beijing Bioprocess Key Laboratory, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, PR China.
Meng
M
Wang
State Key Laboratory of Organic-Inorganic Composites, Beijing University of Chemical Technology, Beijing 100029, PR China.
Lei
L
Shao
Beijing Bioprocess Key Laboratory, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, PR China. Electronic address: dengli@mail.buct.edu.cn.
Li
L
Deng
Beijing Bioprocess Key Laboratory, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, PR China.
Kaili
K
Nie
Beijing Bioprocess Key Laboratory, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, PR China; State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, Beijing 100029, PR China.
Fang
F
Wang
D055162:Biocatalysis; D056804:Biofuels; D019149:Bioreactors; D002175:Candida / Q000201:enzymology; D004951:Esterification; D005227:Fatty Acids / Q000737:chemistry / Q000378:metabolism*; D005656:Fungal Proteins / Q000378:metabolism; D006868:Hydrolysis; D008049:Lipase / Q000378:metabolism*; D000432:Methanol / Q000737:chemistry*; D012997:Solvents / Q000737:chemistry
D056804:Biofuels; D005227:Fatty Acids; D005656:Fungal Proteins; D012997:Solvents; D008049:Lipase; D000432:Methanol
Biodiesel; Candida sp. 99-125 lipase; Esterification; Hydrolysis; Rotating packed bed reactor