Sphk1 promotes breast epithelial cell proliferation via NF-κB-p65-mediated cyclin D1 expression.
Lipid metabolism is crucially involved with the promotion of malignant progression and metastasis in various cancers. Growing evidence suggests that many types of cancers express high levels of sphingosine kinase 1 (Sphk1), which is known to mediate cell proliferation We hypothesized that Sphk1/sphingosine-1-phosphate (S1P) signaling contributes to tumor progression. In MCF10A and MCF10A-Sphk1 breast epithelial cells, we used TNF-α to activate the Sphk1/S1P pathway and the measured expression levels of NF-κBp65 and cyclin D1 mRNA and protein in the presence and absence of an NF-κB-p65 inhibitor. Chromatin immunoprecipitation assays were performed to determine whether NF-κB-p65 binds to the cyclin D1 promoter. We found that overexpression of Sphk1 induced NF-κB-p65 activation, increased expression of cyclin D1, shortened the cell division cycle, and thus promoted proliferation of breast epithelial cells. These findings provide insight into the mechanism by which an Sphk1/NF-κB-p65/cyclin D1 signaling pathway mediates cell proliferation.
Breast Disease Center, Southwest Hospital, Third Military Medical University, Chongqing 400038, China.
Shifei
S
Li
Breast Disease Center, Southwest Hospital, Third Military Medical University, Chongqing 400038, China.
Yan
Y
Zhou
Breast Disease Center, Southwest Hospital, Third Military Medical University, Chongqing 400038, China.
Xiaodong
X
Zheng
Breast Disease Center, Southwest Hospital, Third Military Medical University, Chongqing 400038, China.
Xiujuan
X
Wu
Breast Disease Center, Southwest Hospital, Third Military Medical University, Chongqing 400038, China.
Yueyang
Y
Liang
Breast Disease Center, Southwest Hospital, Third Military Medical University, Chongqing 400038, China.
Shushu
S
Wang
Breast Disease Center, Southwest Hospital, Third Military Medical University, Chongqing 400038, China.
Yi
Y
Zhang
D001665:Binding Sites; D002453:Cell Cycle; D002460:Cell Line; D049109:Cell Proliferation; D019938:Cyclin D1 / Q000235:genetics / Q000378:metabolism*; D004847:Epithelial Cells / Q000201:enzymology* / Q000473:pathology; D005260:Female; D006801:Humans; D008246:Lysophospholipids / Q000378:metabolism; D042361:Mammary Glands, Human / Q000201:enzymology* / Q000473:pathology; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000235:genetics / Q000378:metabolism*; D011401:Promoter Regions, Genetic; D015398:Signal Transduction; D013110:Sphingosine / Q000031:analogs & derivatives / Q000378:metabolism; D013997:Time Factors; D051996:Transcription Factor RelA / Q000235:genetics / Q000378:metabolism*; D014158:Transcription, Genetic; D000099157:Sphingosine Kinase
D019938:Cyclin D1; D008246:Lysophospholipids; D017853:Phosphotransferases (Alcohol Group Acceptor); D013110:Sphingosine; D051996:Transcription Factor RelA; D000099157:Sphingosine Kinase; C530104:CCND1 protein, human; C498143:RELA protein, human; C060506:sphingosine 1-phosphate
CyclinD1; NF-κBp65; Sphk1; cell proliferation