Intestinal bacterium-derived cyp27a1 prevents colon cancer cell apoptosis.
The pathogenesis of metastasis of colon cancer (Cca) is to be further investigated. The dysfunction of apoptotic mechanism plays a role in the cancer cell over growth. This study tests a hypothesis by which intestinal bacterium-derived cyp27a1 prevents apoptosis in colon cancer cells. In this study, the levels of cyp27a1 in human stool samples were assessed by enzyme-linked immunosorbent assay. The apoptosis of Cca cells was observed by flow cytometry. The expression of cyp27a1 was assessed by real time RT-PCR and Western blotting. We observed higher levels of cyp27a1 in the stool samples of Cca patients than that from healthy subjects. Cca colon epithelial biopsy contained high levels of cyp27a1 protein, but not the cyp27a1 mRNA. Cyp27a1 prevented Cca cell apoptosis induced by vitamin D3. In conclusion, intestinal bacterium-derived cyp27a1 facilitates Cca survival by inhibiting Cca cell apoptosis.
Department of General Surgery, The Fourth Affiliated Hospital of Harbin Medical University Harbin 150001, China.
Yan-Chao
YC
Ji
Department of General Surgery, The Fourth Affiliated Hospital of Harbin Medical University Harbin 150001, China.
Chang
C
Liu
Department of General Surgery, The Fourth Affiliated Hospital of Harbin Medical University Harbin 150001, China.
Xia
X
Zhang
Department of General Surgery, The Fourth Affiliated Hospital of Harbin Medical University Harbin 150001, China.
Cheng-Sen
CS
Zhang
Department of General Surgery, The Fourth Affiliated Hospital of Harbin Medical University Harbin 150001, China.
Dong
D
Wang
Department of General Surgery, The Fourth Affiliated Hospital of Harbin Medical University Harbin 150001, China.
Yan
Y
Zhang
Colon cancer; Cyp27a1; apoptosis; vitamin D