A Case-control Study on the Relationship betweenMycoplasma genitaliumInfection in Women with Normal Pregnancy and Spontaneous Abortion using Polymerase Chain Reaction.
OBJECTIVE
Mycoplasma genitaliuminfections are suggested as causes of a number of pathological outcomes in pregnant women. The aim of this study was to evaluate the frequency ofM. genitaliuminfections among pregnant women and its association with spontaneous abortion.


METHODS
In this case-control study we included 109 women with spontaneous abortion with a gestational age of 10-20 weeks (patients), and 109 women with normal pregnancy with a gestational age of 20-37 weeks (controls) in Sanandaj, Iran. Using specific primers and extracted DNA from endocervical swabs, a polymerase chain reaction was conducted for the detection ofM. genitaliuminfection in both groups.


RESULTS
The frequency ofM. genitaliuminfection in patient and control groups was one (0.91%) and three (2.75%), respectively. In both control and patient groups using Fisher test, no association between mycoplasma infection and spontaneous abortion was seen.


CONCLUSIONS
M. genitaliummay be positive in the genital tract of some pregnant women but was not associated with spontaneous abortion. Further powerful studies with larger sample sizes are needed for the determination of a possible role ofM. genitaliumin pregnancy outcomes and spontaneous abortion.
Cellular and Molecular Research Center, Kurdistan University of Medical Sciences, Sanandaj, Iran; Microbiology Department, Kurdistan University of Medical Sciences, Sanandaj, Iran.
Rashid
R
Ramazanzadeh
Cellular and Molecular Research Center, Kurdistan University of Medical Sciences, Sanandaj, Iran; Microbiology Department, Kurdistan University of Medical Sciences, Sanandaj, Iran.
Mazaher
M
Khodabandehloo
Social Determinants of Health Research Center, Kurdistan University of Medical Sciences, Sanandaj, Iran.
Fariba
F
Farhadifar
Cellular and Molecular Research Center, Kurdistan University of Medical Sciences, Sanandaj, Iran; Microbiology Department, Kurdistan University of Medical Sciences, Sanandaj, Iran; Student Research Committee, Kurdistan University of Medical Sciences, Sanandaj, Iran.
Samaneh
S
Rouhi
Cellular and Molecular Research Center, Kurdistan University of Medical Sciences, Sanandaj, Iran; Microbiology Department, Kurdistan University of Medical Sciences, Sanandaj, Iran.
Amjad
A
Ahmadi
Department of Pathology and Medical Laboratory Sciences, Kurdistan University of Medical Sciences, Sanandaj, Iran.
Shaho
S
Menbari
Social Determinants of Health Research Center, Kurdistan University of Medical Sciences, Sanandaj, Iran.
Fariba
F
Fallahi
Molecular Biology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran.
Reza
R
Mirnejad
Mycoplasma genitalium; normal pregnancy; spontaneous abortion; women