Aluminum Trichloride Inhibited Osteoblastic Proliferation and Downregulated the Wnt/β-Catenin Pathway.
Aluminum (Al) exposure inhibits bone formation. Osteoblastic proliferation promotes bone formation. Therefore, we inferred that Al may inhibit bone formation by the inhibition of osteoblastic proliferation. However, the effects and molecular mechanisms of Al on osteoblastic proliferation are still under investigation. Osteoblastic proliferation can be regulated by Wnt/β-catenin signaling pathway. To investigate the effects of Al on osteoblastic proliferation and whether Wnt/β-catenin signaling pathway is involved in it, osteoblasts from neonatal rats were cultured and exposed to 0, 0.4 mM (1/20 IC50), 0.8 mM (1/10 IC50), and 1.6 mM (1/5 IC50) of aluminum trichloride (AlCl3) for 24 h, respectively. The osteoblastic proliferation rates; Wnt3a, lipoprotein receptor-related protein 5 (LRP-5), T cell factor 1 (TCF-1), cyclin D1, and c-Myc messenger RNA (mRNA) expressions; and p-glycogen synthase kinase 3β (GSK3β), GSK3β, and β-catenin protein expressions indicated that AlCl3inhibited osteoblastic proliferation and downregulated Wnt/β-catenin signaling pathway. In addition, the AlCl3concentration was negatively correlated with osteoblastic proliferation rates and the mRNA expressions of Wnt3a, c-Myc, and cyclin D1, while the osteoblastic proliferation rates were positively correlated with mRNA expressions of Wnt3a, c-Myc, and cyclin D1. Taken together, these findings indicated that AlCl3inhibits osteoblastic proliferation may be associated with the inactivation of Wnt/β-catenin signaling pathway.
College of Veterinary Medicine, Northeast Agricultural University, Harbin, 150030, China.
Wanyue
W
Huang
College of Veterinary Medicine, Northeast Agricultural University, Harbin, 150030, China.
Peiyan
P
Wang
College of Veterinary Medicine, Northeast Agricultural University, Harbin, 150030, China.
Tongtong
T
Shen
College of Animal Science, Fujian Agricultural and Forestry University, Fuzhou, 350002, China.
Chongwei
C
Hu
College of Veterinary Medicine, Northeast Agricultural University, Harbin, 150030, China.
Yanfei
Y
Han
College of Veterinary Medicine, Northeast Agricultural University, Harbin, 150030, China.
Miao
M
Song
College of Veterinary Medicine, Northeast Agricultural University, Harbin, 150030, China.
Yu
Y
Bian
College of Veterinary Medicine, Northeast Agricultural University, Harbin, 150030, China. liyanfei@neau.edu.cn.
Yanfei
Y
Li
Institute of Special Animal and Plant Sciences, Chinese Academy of Agricultural Sciences, Changchun, 130112, China. zhuyanzhu@caas.cn.
Yanzhu
Y
Zhu
D000077410:Aluminum Chloride; D017607:Aluminum Compounds / Q000633:toxicity*; D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D002712:Chlorides / Q000633:toxicity*; D004305:Dose-Response Relationship, Drug; D015536:Down-Regulation / Q000187:drug effects*; D010006:Osteoblasts / Q000187:drug effects* / Q000473:pathology; D051381:Rats; D017207:Rats, Sprague-Dawley; D013329:Structure-Activity Relationship; D060449:Wnt Signaling Pathway / Q000187:drug effects*; D051176:beta Catenin / Q000037:antagonists & inhibitors* / Q000378:metabolism
D017607:Aluminum Compounds; D002712:Chlorides; D051176:beta Catenin; D000077410:Aluminum Chloride
Aluminum trichloride; Osteoblastic proliferation; Rat osteoblasts; Wnt/β-catenin signaling pathway