Apoptotic effect of sodium acetate on a human gastric adenocarcinoma epithelial cell line.
The objective of this study was to investigate the effect of sodium acetate on the viability of the human gastric adenocarcinoma (AGS) epithelial cell line. AGS cells were exposed to a range of concentrations of sodium acetate for different periods of time, and the sodium acetate-induced cytotoxic effects, including cell viability, DNA fragmentation, apoptotic gene expression, and caspase activity, were assessed. The changes in these phenotypes were quantified by performing a lactate dehydrogenase cell viability assay, annexin V staining, terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL), and several caspase activity assays. In vitro studies demonstrated that the cytotoxicity of sodium acetate on the AGS cell line were dose- and time-dependent manners. No differences were found between the negative control and sodium acetate-treated cells stained with annexin V and subjected to the TUNEL assay. However, caspase-3 activity was increased in AGS cells exposed to sodium acetate. Overall, it was concluded that sodium acetate exerted an apoptotic effect in AGS cells via a caspase-dependent apoptotic pathway.
Inner Mongolia Medical University, Hohhot, China.
Y
Y
Xia
Capital Medical University, Beijing, China.
X L
XL
Zhang
Inner Mongolia Vocational College of Chemical Engineering, Hohhot, China.
F
F
Jin
Inner Mongolia Medical University, Hohhot, China.
Q X
QX
Wang
Inner Mongolia Medical University, Hohhot, China.
R
R
Xiao
Inner Mongolia People's Hospital, Hohhot, China.
Z H
ZH
Hao
International Mongolia Hospital of Inner Mongolia, China.
Q D
QD
Gui
Inner Mongolia Medical University, Hohhot, China 2573479397@qq.com.
J
J
Sun
D000230:Adenocarcinoma / Q000201:enzymology / Q000473:pathology*; D017209:Apoptosis / Q000187:drug effects*; D020169:Caspases / Q000378:metabolism; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D053938:DNA Fragmentation / Q000187:drug effects; D004847:Epithelial Cells / Q000187:drug effects / Q000201:enzymology / Q000473:pathology*; D006657:Histones / Q000378:metabolism; D006801:Humans; D019346:Sodium Acetate / Q000494:pharmacology*; D013274:Stomach Neoplasms / Q000201:enzymology / Q000473:pathology*
D006657:Histones; D019346:Sodium Acetate; D020169:Caspases