Heavy-ion beam mutagenesis identified an essential gene for chloroplast development under cold stress conditions during both early growth and tillering stages in rice.
We isolated a cold sensitive virescent1 (csv1) mutant from a rice (Oryza sativa L.) population mutagenized by carbon ion irradiation. The mutant exhibited chlorotic leaves during the early growth stages, and produced normal green leaves as it grew. The growth of csv1 plants displayed sensitivity to low temperatures. In addition, the mutant plants that were transferred to low temperatures at the fifth leaf stage produced chlorotic leaves subsequently. Genetic and molecular analyses revealed translocation of a 13-kb genomic fragment that disrupted the causative gene (CSV1; LOC_Os05g34040). CSV1 encodes a plastid-targeted oxidoreductase-like protein conserved among land plants, green algae, and cyanobacteria. Furthermore, CSV1 transcripts were more abundant in immature than in mature leaves, and they did not markedly increase or decrease with temperature. Taken together, our results indicate that CSV1 supports chloroplast development under cold stress conditions, in both the early growth and tillering stages in rice.
a Nishina Center for Accelerator-Based Science, RIKEN, Wako , Japan.
Ryouhei
R
Morita
a Nishina Center for Accelerator-Based Science, RIKEN, Wako , Japan.
Mayu
M
Nakagawa
a Nishina Center for Accelerator-Based Science, RIKEN, Wako , Japan.
Hinako
H
Takehisa
a Nishina Center for Accelerator-Based Science, RIKEN, Wako , Japan.
Yoriko
Y
Hayashi
a Nishina Center for Accelerator-Based Science, RIKEN, Wako , Japan.
Hiroyuki
H
Ichida
a Nishina Center for Accelerator-Based Science, RIKEN, Wako , Japan.
Sachiko
S
Usuda
a Nishina Center for Accelerator-Based Science, RIKEN, Wako , Japan.
Katsunori
K
Ichinose
a Nishina Center for Accelerator-Based Science, RIKEN, Wako , Japan.
Hiroshi
H
Abe
a Nishina Center for Accelerator-Based Science, RIKEN, Wako , Japan.
Yuki
Y
Shirakawa
a Nishina Center for Accelerator-Based Science, RIKEN, Wako , Japan.|b Graduate School of Agricultural Science, Tohoku University , Sendai , Japan.
Tadashi
T
Sato
a Nishina Center for Accelerator-Based Science, RIKEN, Wako , Japan.|c Faculty of Science and Technology , Sophia University , Tokyo , Japan.
Makoto T
MT
Fujiwara
d Faculty of Science , University of the Ryukyus , Nishihara, Japan.
Ryuuichi D
RD
Itoh
a Nishina Center for Accelerator-Based Science, RIKEN, Wako , Japan.
Tomoko
T
Abe
D002736:Chloroplasts / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism / Q000648:ultrastructure; D058639:Cold-Shock Response / Q000187:drug effects / Q000235:genetics*; D017124:Conserved Sequence; D004579:Electron Transport / Q000187:drug effects / Q000235:genetics; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D020450:Heavy Ions; D042541:Intracellular Space / Q000187:drug effects / Q000378:metabolism; D016296:Mutagenesis / Q000187:drug effects*; D009154:Mutation; D012275:Oryza / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development* / Q000502:physiology; D010788:Photosynthesis / Q000187:drug effects / Q000235:genetics; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D018087:Plastids / Q000187:drug effects / Q000235:genetics; D021381:Protein Transport
D010940:Plant Proteins
carbon ion-induced mutant; chloroplast development; low temperature; rice (Oryza sativa L.); virescent