A Comparative Metabolomics Approach Reveals Early Biomarkers for Metabolic Response to Acute Myocardial Infarction.
Discovery of novel biomarkers is critical for early diagnosis of acute coronary syndrome (ACS). Serum metabolite profiling of ST-elevation myocardial infarction (STEMI), unstable angina (UA) and healthy controls was performed using gas chromatography mass spectrometry (GC/MS), solid-phase microextraction coupled to gas chromatography mass spectrometry (SPME-GC/MS) and nuclear magnetic resonance (1H-NMR). Multivariate data analysis revealed a metabolic signature that could robustly discriminate STEMI patients from both healthy controls and UA patients. This panel of biomarkers consisted of 19 metabolites identified in the serum of STEMI patients. One of the most intriguing biomarkers among these metabolites is hydrogen sulfide (H2S), an endogenous gasotransmitter with profound effect on the heart. Serum H2S absolute levels were further investigated using a quantitative double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). This highly sensitive immunoassay confirmed the elevation of serum H2S in STEMI patients. H2S level discriminated between UA and STEMI groups, providing an initial insight into serum-free H2S bioavailability during ACS. In conclusion, the current study provides a detailed map illustrating the most predominant altered metabolic pathways and the biochemical linkages among the biomarker metabolites identified in STEMI patients. Metabolomics analysis may yield novel predictive biomarkers that will potentially allow for an earlier medical intervention.
Department of Pharmaceutical Biology, Faculty of Pharmacy &Biotechnology, The German University in Cairo, Egypt.
Sara E
SE
Ali
Department of Pharmacognosy, Faculty of Pharmacy, Cairo University, Cairo, 11562, Egypt.
Mohamed A
MA
Farag
Department of Cardiovascular Sciences, Atherosclerosis and Metabolism Unit, KatholiekeUniversiteit Leuven, Belgium.
Paul
P
Holvoet
Department of Pharmaceutical Chemistry, Faculty of Pharmacy &Biotechnology, The German University in Cairo, Egypt.
Rasha S
RS
Hanafi
Department of Biochemistry, Faculty of Pharmacy &Biotechnology, The German University in Cairo, Egypt.
Mohamed Z
MZ
Gad
D054058:Acute Coronary Syndrome / Q000175:diagnosis*; D000328:Adult; D000368:Aged; D000789:Angina, Unstable / Q000097:blood / Q000378:metabolism*; D015415:Biomarkers / Q000097:blood; D042241:Early Diagnosis; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D006862:Hydrogen Sulfide / Q000097:blood*; D008297:Male; D055432:Metabolomics / Q000379:methods*; D008875:Middle Aged; D066244:Proton Magnetic Resonance Spectroscopy; D000072657:ST Elevation Myocardial Infarction / Q000097:blood / Q000378:metabolism*
D015415:Biomarkers; D006862:Hydrogen Sulfide