Induction of humoral and cellular immune response to hepatitis B virus (HBV) vaccine can be upregulated by CpG oligonucleotides complexed with Dectin-1 ligand.
A persistent hepatitis B virus (HBV) infection is characterized by a lack of or a weak immune response to HBV, which may be reflective of tolerance to HBV. Efficient induction of HBV-specific immune response leads to the clearance of HBV in patients with a chronic HBV infection. CpG oligodeoxynucleotides (ODN) has a powerful adjuvant effect in HBV vaccination. A recent report demonstrated that the immunization by B/K CpG ODN (K3) wrapped by the nonagonistic Dectin-1 ligand, schizophyllan (SPG), namely K3-SPG, was more effective in the induction of antigen-specific immune response than that by K3. In this study, we examined the efficacy of K3-SPG as a HBV vaccine adjuvant. Wild-type (WT) mice and HBV transgenic (HBV-Tg) mice were subcutaneously immunized with hepatitis B surface antigen (HBsAg) alone, HBsAg and K3, or HBsAg and K3-SPG. The vaccination with HBsAg and K3-SPG significantly enhanced humoral and cellular immune response to HBV antigen compared to the other vaccinations in WT and HBV-Tg mice. K3-SPG induced the accumulation of dendritic cells (DCs) into draining lymph node and the activation of DCs. The expression of cytokines and chemokines related to Th1 and Th2 responses was upregulated after the vaccination including with K3-SPG. In conclusion, these results indicated that the vaccination using K3-SPG may overcome tolerance even in patients with chronic HBV infection.
Department of Informative Clinical Medicine, Gifu University Graduate School of Medicine, Gifu, Gifu, Japan.
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Ito
Department of Informative Clinical Medicine, Gifu University Graduate School of Medicine, Gifu, Gifu, Japan.
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Ando
Department of Informative Clinical Medicine, Gifu University Graduate School of Medicine, Gifu, Gifu, Japan.
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Nakamura
Department of Informative Clinical Medicine, Gifu University Graduate School of Medicine, Gifu, Gifu, Japan.
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Ishida
Department of Informative Clinical Medicine, Gifu University Graduate School of Medicine, Gifu, Gifu, Japan.
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Kanbe
Laboratory of Adjuvant Innovation, National Institutes of Biomedical Innovation, Health and Nutrition, Ibaraki, Osaka, Japan.|Laboratory of Vaccine Science, World Premier International Research Center, Immunology Frontier Research Center, Osaka University, Suita, Osaka, Japan.
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Kobiyama
Laboratory of Adjuvant Innovation, National Institutes of Biomedical Innovation, Health and Nutrition, Ibaraki, Osaka, Japan.|Laboratory of Vaccine Science, World Premier International Research Center, Immunology Frontier Research Center, Osaka University, Suita, Osaka, Japan.
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Yamamoto
Laboratory of Adjuvant Innovation, National Institutes of Biomedical Innovation, Health and Nutrition, Ibaraki, Osaka, Japan.|Laboratory of Vaccine Science, World Premier International Research Center, Immunology Frontier Research Center, Osaka University, Suita, Osaka, Japan.
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Ishii
Department of Tumor Pathology, Gifu University Graduate School of Medicine, Gifu, Gifu, Japan.
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Hara
Department of Informative Clinical Medicine, Gifu University Graduate School of Medicine, Gifu, Gifu, Japan.
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Seishima
Department of Medical Technology, Nagoya University School of Health Sciences, Nagoya, Aichi, Japan.
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Ishikawa
D000276:Adjuvants, Immunologic / Q000008:administration & dosage*; D000818:Animals; D006514:Hepatitis B Surface Antigens / Q000008:administration & dosage / Q000276:immunology; D017325:Hepatitis B Vaccines / Q000008:administration & dosage* / Q000276:immunology*; D007111:Immunity, Cellular; D056724:Immunity, Humoral; D007279:Injections, Subcutaneous; D008297:Male; D051379:Mice; D009838:Oligodeoxyribonucleotides / Q000008:administration & dosage*; D012566:Sizofiran / Q000008:administration & dosage*
D000276:Adjuvants, Immunologic; C408982:CPG-oligonucleotide; D006514:Hepatitis B Surface Antigens; D017325:Hepatitis B Vaccines; D009838:Oligodeoxyribonucleotides; D012566:Sizofiran

HBV
; CpG ODN; immune response; vaccination