Lipid Membrane Encapsulation of a 3D DNA Nano Octahedron.
Structural DNA nanotechnology methods such as DNA origami allow for the synthesis of highly precise nanometer-scale materials (Rothemund, Nature 440:297-302, 2006; Douglas et al., Nature 459:414-418, 2009). These offer compelling advantages for biomedical applications. Such materials can suffer from structural instability in biological environments due to denaturation and nuclease digestion (Hahn et al., ACS Nano 2014; Perrault and Shih, ACS Nano 8:5132-5140, 2014). Encapsulation of DNA nanostructures in a lipid membrane compartmentalizes them from their environment and prevents denaturation and nuclease digestion (Perrault and Shih, ACS Nano 8:5132-5140, 2014). Here, we describe the encapsulation of a 50 nm DNA nanostructure having the geometry of a wireframe octahedron in a phospholipid membrane containing poly-(ethylene glycol), resulting in biocompatible DNA nanostructures.
Wyss Institute for Biologically Inspired Engineering, Harvard Medical School, CLSB 5th Floor, Blackfan Circle, Boston, MA, 02115, USA. Steven.Perrault@Wyss.Harvard.Edu.|Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA, 02115, USA. Steven.Perrault@Wyss.Harvard.Edu.|Department of Cancer Biology, Dana Farber Cancer Institute, Boston, MA, 02115, USA. Steven.Perrault@Wyss.Harvard.Edu.
Steven D
SD
Perrault
Wyss Institute for Biologically Inspired Engineering, Harvard Medical School, CLSB 5th Floor, Blackfan Circle, Boston, MA, 02115, USA.|Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA, 02115, USA.|Department of Cancer Biology, Dana Farber Cancer Institute, Boston, MA, 02115, USA.
William M
WM
Shih
D004247:DNA / Q000737:chemistry*; D008563:Membrane Lipids / Q000737:chemistry*; D008566:Membranes / Q000737:chemistry*; D049329:Nanostructures / Q000737:chemistry*; D036103:Nanotechnology / Q000379:methods; D011092:Polyethylene Glycols / Q000737:chemistry
D008563:Membrane Lipids; D011092:Polyethylene Glycols; D004247:DNA
DNA; Liposome; NanoOctahedron; Nanotechnology; Vesicle