Immunohistochemical Localization of GFAP and Glutamate Regulatory Proteins in Chick Retina and Their Levels of Expressions in Altered Photoperiods.
Moderate to intense light is reported to damage the chick retina, which is cone dominated. Light damage alters neurotransmitter pools, such as those of glutamate. Glutamate level in the retina is regulated by glutamate-aspartate transporter (GLAST) and glutamine synthetase (GS). We examined immunolocalization patterns and the expression levels of both markers and of glial fibrillary acidic protein (GFAP, a marker of neuronal stress) in chick retina exposed to 2000 lux under 12-h light:12-h dark (12L:12D; normal photoperiod), 18L:6D (prolonged photoperiod), and 24L:0D (constant light) at post-hatch day 30. Retinal damage (increased death of photoreceptors and inner retinal neurons and Müller cell hypertrophy) and GFAP expression in Müller cells were maximal in 24L:0D condition compared to that seen in 12L:12D and 18L:6D conditions. GS was present in Müller cells and GLAST expressed in Müller cell processes and photoreceptor inner segments. GLAST expression was decreased in 24L:0D condition, and the expression levels between 12L:12D and 18L:6D, though increased marginally, were statistically insignificant. Similar was the case with GS expression that significantly decreased in 24L:0D condition. Our previous study with chicks exposed to 2000 lux reported increased retinal glutamate level in 24L:0D condition. The present results indicate that constant light induces decreased expressions of GLAST and GS, a condition that might aggravate glutamate-mediated neurotoxicity and delay neuroprotection in a cone-dominated retina.
Department of Anatomy, All India Institute of Medical Sciences, Room No. 1029, New Delhi, 110029, India.
Kumar Abhiram
KA
Jha
Department of Anatomy, All India Institute of Medical Sciences, Room No. 1029, New Delhi, 110029, India. tapas_nag@aiims.edu.
Tapas C
http://orcid.org/0000-0002-6962-0844
TC
Nag
Department of Anatomy, All India Institute of Medical Sciences, Room No. 1029, New Delhi, 110029, India.
Shashi
S
Wadhwa
Department of Anatomy, All India Institute of Medical Sciences, Room No. 1029, New Delhi, 110029, India.
Tara Sankar
TS
Roy
D027322:Amino Acid Transport System X-AG / Q000378:metabolism*; D000818:Animals; D048430:Cell Shape / Q000528:radiation effects; D002645:Chickens / Q000378:metabolism*; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism*; D005974:Glutamate-Ammonia Ligase / Q000378:metabolism*; D018698:Glutamic Acid / Q000378:metabolism*; D007150:Immunohistochemistry; D008027:Light; D009412:Nerve Fibers / Q000378:metabolism / Q000528:radiation effects / Q000648:ultrastructure; D017440:Photoperiod; D012160:Retina / Q000166:cytology / Q000378:metabolism* / Q000528:radiation effects / Q000648:ultrastructure
D027322:Amino Acid Transport System X-AG; D005904:Glial Fibrillary Acidic Protein; D018698:Glutamic Acid; D005974:Glutamate-Ammonia Ligase
Chick retina; GFAP; Glutamate–aspartate transporter; Glutamine synthetase; Light damage; Müller cells