Abnormal expression of p190RhoGAP in colorectal cancer patients with poor survival.
OBJECTIVE
This study aims to investigate the expression and clinical significance of p190RhoGAP, a member of the RhoGAP family, in colorectal cancer (CRC).


METHODS
The expression p190RhoGAP was detected by RT-PCR, western blot (WB) and immunohistochemistry (IHC) in 14 paired CRCs and matched non-cancerous mucosal tissues. The protein content of p190RhoGAP was identified in 114 CRCs by IHC. In addition, the association of the expression of p190RhoGAP with carcinogenesis, distant metastasis and prognosis was further evaluated.


RESULTS
In 14 paired fresh tissues, the mRNA (P<0.0001) and protein (P= 0.003) expression levels of p190RhoGAP were significantly higher in primary CRCs than in paired non-cancerous mucosal tissues; and was consistent with WB results. The expression of p190RhoGAP increased from normal mucosa to adenoma, and became even greater in primary carcinoma (P= 0.001). The expression level of p190RhoGAP was highest in liver metastasis compared to primary carcinoma (P= 0.028). The incidence of p190RhoGAP expression-positive cases was 58.77% in 114 CRC tissues. Furthermore, the enhanced expression of p190RhoGAP was significantly associated with shorter disease-specific survival (P<0.001) and shorter disease-free survival (P<0.001). Cox regression analysis indicated that p190RhoGAP was an independent prognostic parameter for CRC.


CONCLUSIONS
p190RhoGAP may be an independent predictive factor for the prognosis of CRC, and the abnormal expression of p190RhoGAP may play a crucial role in colorectal carcinogenesis and distant metastasis.
Colorectal Surgery, The Affiliated Tumor Hospital of Harbin Medical University Harbin, Heilongjiang, China.
Li
L
Li
Colorectal Surgery, The Affiliated Tumor Hospital of Harbin Medical University Harbin, Heilongjiang, China.
Yong-Min
YM
Li
Department of Pediatrics, The Second Affiliated Hospital of Harbin Medical University Harbin, Heilongjiang, China.
Ping
P
Zhou
Colorectal Surgery, Cancer Hospital Chinese Academy of Medical Sciences Beijing, China.
Xi-Shan
XS
Wang
Colorectal Surgery, The Second Affiliated Hospital of Harbin Medical University Harbin, Heilongjiang, China.
Gui-Yu
GY
Wang
Breast Surgery, The Affiliated Tumor Hospital of Harbin Medical University Harbin, Heilongjiang, China.
Xu-Hai
XH
Zhao
Colorectal Surgery, The Affiliated Tumor Hospital of Harbin Medical University Harbin, Heilongjiang, China.
Bin-Bin
BB
Cui
Breast Surgery, The Affiliated Tumor Hospital of Harbin Medical University Harbin, Heilongjiang, China.
Yan-Lu
YL
Ren
Department of General Surgery, The No.4 Affiliated Hospital of Harbin Medical University Harbin, Heilongjiang, China.
Xin-Shu
XS
Dong
Department of General Surgery, Daqing Oilfield General Hospital Daqing, Heilongjiang, China.
Zhi-Qi
ZQ
Chen
colorectal cancer; metastasis; p190rhogap; poor survival