Senescence Phenotypes Induced by Ras in Primary Cells.
Cellular senescence is defined as a state of stable cell-cycle arrest that is distinct from quiescence and terminal differentiation. Many stimuli can induce senescence, including telomere shortening and oncogene activation. The phenotypes elicited by pro-senescent signals can be heterogeneous depending on the stimulus and the cell type affected. To date, there is not a definitive marker that can ubiquitously and specifically mark all senescent cells. Therefore, several independent markers must be utilized to ascertain the senescent state of a cell or group of cells. Here, we describe common assays used to assess oncogenic Ras-induced senescence.
Department of Biochemistry and Molecular Pharmacology, Perlmutter Cancer Institute, New York University School of Medicine, 550 First Avenue, New York, NY, 10016, USA.
Lena
L
Lau
Department of Biochemistry and Molecular Pharmacology, Perlmutter Cancer Institute, New York University School of Medicine, 550 First Avenue, New York, NY, 10016, USA. gregory.david@ynyumc.org.
Gregory
G
David
D015415:Biomarkers; D002453:Cell Cycle / Q000235:genetics; D002461:Cell Line, Transformed; D002478:Cells, Cultured; D016922:Cellular Senescence / Q000502:physiology*; D042002:Chromatin Assembly and Disassembly; D005347:Fibroblasts / Q000378:metabolism; D005786:Gene Expression Regulation; D006570:Heterochromatin / Q000235:genetics / Q000378:metabolism; D006801:Humans; D015689:Oncogene Protein p21(ras) / Q000235:genetics* / Q000378:metabolism*; D010641:Phenotype; D061251:Primary Cell Culture; D013381:Subtilisins / Q000235:genetics / Q000378:metabolism; D001616:beta-Galactosidase / Q000378:metabolism
D015415:Biomarkers; D006570:Heterochromatin; D001616:beta-Galactosidase; D013381:Subtilisins; D015689:Oncogene Protein p21(ras)
Oncogene; Primary fibroblasts; Ras; SA-βgal; SAHF; SASP; Senescence