LapF and Its Regulation by Fis Affect the Cell Surface Hydrophobicity of Pseudomonas putida.
The ability of bacteria to regulate cell surface hydrophobicity is important for the adaptation to different environmental conditions. The hydrophobicity of cell surface can be determined by several factors, including outer membrane and surface proteins. In this study, we report that an adhesin LapF influences cell surface hydrophobicity of Pseudomonas putida. Cells lacking LapF are less hydrophobic than wild-type cells in stationary growth phase. Moreover, the overexpression of the global regulator Fis decreases surface hydrophobicity by repressing the expression of lapF. Flow cytometry analysis revealed that bacteria producing LapF are more viable when confronted with methanol (a hydrophilic compound) but are more susceptible to 1-octanol (a hydrophobic compound). Thus, these results revealed that LapF is the hydrophobicity factor for the cell surface of P. putida.
Institute of Molecular and Cell Biology, University of Tartu, Tartu, Estonia.
Andrio
A
Lahesaare
Institute of Molecular and Cell Biology, University of Tartu, Tartu, Estonia.
Hanna
H
Ainelo
Institute of Molecular and Cell Biology, University of Tartu, Tartu, Estonia.
Annika
A
Teppo
Institute of Molecular and Cell Biology, University of Tartu, Tartu, Estonia.
Maia
M
Kivisaar
Helmholtz Centre for Environmental Research-UFZ, Department of Environmental Biotechnology, Leipzig, Germany.
Hermann J
HJ
Heipieper
Institute of Molecular and Cell Biology, University of Tartu, Tartu, Estonia.
Riho
R
Teras
D001422:Bacterial Adhesion; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D015964:Gene Expression Regulation, Bacterial; D055786:Gene Knockout Techniques; D057927:Hydrophobic and Hydrophilic Interactions; D000432:Methanol / Q000633:toxicity; D009876:Operon / Q000235:genetics; D016958:Pseudomonas putida / Q000166:cytology* / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*
D001426:Bacterial Proteins; D000432:Methanol