Identification of Novel HLA-A*0201-Restricted CTL Epitopes in Chinese Vitiligo Patients.
Generalized vitiligo is an autoimmune disease characterized by melanocyte loss, which results in patchy depigmentation of skin and hair. Recent studies suggested the key role of CD8+T lymphocytes for mediating immune response in vitiligo through melanocyte differentiation antigens, including tyrosinase, gp100 and MelanA/Mart-1. However, the specific epitopes of these auto-antigens are still unknown. In our study, we predicted the possible HLA-A*0201-restricted nonapeptides overlaying the full-length amino acid sequences of these three known antigens and investigated the lymphocytes reactivity to these nonapeptides by Elispot assay. In addition, we evaluated the abilities of these nonapeptides to activate CD8+T cells. We screened out 5 possible epitopes originated from tyrosinase and gp100, numbered P28, P41, P112, P118 and P119. Among these 5 epitopes, notably, P28 and P119 played the dominant role in activating CTLs, with a significant increase in proliferation rate and Interferon-γ (IFN-γ) production of CD8+T cells. Nevertheless, antigen-specific T cell reactivity was not detected in MelanA/Mart-1 peptides. Our studies identified two novel epitopes originated from proteins of gp100 and tyrosinase, which may have implications for the development of immunotherapies for vitiligo.
Department of Dermatology, Xijing Hospital, Fourth Military Medical University, 127 Changle West Road, Xi'an, shaanxi, 710032, China.
Tingting
T
Cui
Department of Dermatology, Xijing Hospital, Fourth Military Medical University, 127 Changle West Road, Xi'an, shaanxi, 710032, China.
Xiuli
X
Yi
Department of Dermatology, Xijing Hospital, Fourth Military Medical University, 127 Changle West Road, Xi'an, shaanxi, 710032, China.
Sen
S
Guo
Department of Dermatology, Xijing Hospital, Fourth Military Medical University, 127 Changle West Road, Xi'an, shaanxi, 710032, China.
Fubo
F
Zhou
Department of Dermatology, Xijing Hospital, Fourth Military Medical University, 127 Changle West Road, Xi'an, shaanxi, 710032, China.
Ling
L
Liu
Department of Dermatology, Xijing Hospital, Fourth Military Medical University, 127 Changle West Road, Xi'an, shaanxi, 710032, China.
Chunying
C
Li
Department of Dermatology, Xijing Hospital, Fourth Military Medical University, 127 Changle West Road, Xi'an, shaanxi, 710032, China.
Kai
K
Li
Department of Dermatology, Xijing Hospital, Fourth Military Medical University, 127 Changle West Road, Xi'an, shaanxi, 710032, China.
Tianwen
T
Gao
D000293:Adolescent; D000328:Adult; D000595:Amino Acid Sequence; D001324:Autoantigens / Q000737:chemistry*; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology; D049109:Cell Proliferation; D002648:Child; D002681:China; D000939:Epitopes / Q000235:genetics* / Q000276:immunology; D005260:Female; D015789:HLA-A2 Antigen / Q000235:genetics*; D006801:Humans; D007371:Interferon-gamma / Q000378:metabolism; D058965:MART-1 Antigen / Q000737:chemistry; D008297:Male; D008875:Middle Aged; D014442:Monophenol Monooxygenase / Q000737:chemistry; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*; D014820:Vitiligo / Q000235:genetics* / Q000276:immunology; D055815:Young Adult; D058951:gp100 Melanoma Antigen / Q000737:chemistry
D001324:Autoantigens; D000939:Epitopes; C435939:HLA-A*02:01 antigen; D015789:HLA-A2 Antigen; C573415:IFNG protein, human; D058965:MART-1 Antigen; C548667:MLANA protein, human; D058951:gp100 Melanoma Antigen; D007371:Interferon-gamma; D014442:Monophenol Monooxygenase