Combined Effects of in Utero and Adolescent Tobacco Smoke Exposure on Lung Function in C57Bl/6J Mice.
Fetal determinants of airway function, such asin uteroexposure to maternal cigarette smoke (CS), may create a predisposition to adult airflow obstruction and chronic obstructive pulmonary disease (COPD) in adulthood. It has been suggested that active smoking in adolescence and preexisting airflow obstruction have synergistic deleterious effects.



We used a mouse model to investigate whether there is a synergistic effect of exposure to CSin uteroand during adolescence on lung function.



Female C57Bl/6J mice were exposed to CS or to filtered room air during pregnancy. Exposure to CS began 2 weeks before mating and continued until delivery. After birth, the pups were not exposed to CS until day 21 (D21). Between D21 and D49, corresponding to "adolescence," litters were randomized for an additional 4 weeks of exposure to CS. Lung morphometry, lung mechanics, and the expression of genes involved in senescence were evaluated in different subsets of mice on D21 and D49.



In uteroexposure to CS induced significant lung function impairment by D21. CS exposure between D21 and D49 induced significant functional impairment only in mice exposed to CS prenatally. On D49, no difference was observed between subgroups in terms of lung p53, p16, p21, and Bax mRNA levels.



Our findings suggest that prenatal and adolescent CS exposure have a synergistic effect on lung function in mice. The combined effect did not appear to be a consequence of early pulmonary senescence. Citation: Drummond D, Baravalle-Einaudi M, Lezmi G, Vibhushan S, Franco-Montoya ML, Hadchouel A, Boczkowski J, Delacourt C. 2017. Combined effects ofin uteroand adolescent tobacco smoke exposure on lung function in C57Bl/6J mice. Environ Health Perspect 125:392-399; http://dx.doi.org/10.1289/EHP54.
INSERM (Institut National de la Santé et de la Recherche Médicale), U955, Equipe 04, IMRB (Institut Mondor de Recherche Biomédicale), Créteil, France.
David
D
Drummond
Mélissa
M
Baravalle-Einaudi
Guillaume
G
Lezmi
Shamila
S
Vibhushan
Marie-Laure
ML
Franco-Montoya
Alice
A
Hadchouel
Jorge
J
Boczkowski
Christophe
C
Delacourt
D000818:Animals; D004195:Disease Models, Animal; D005260:Female; D008168:Lung / Q000187:drug effects / Q000503:physiopathology*; D018811:Maternal Exposure; D051379:Mice; D008810:Mice, Inbred C57BL; D011247:Pregnancy; D014028:Tobacco Smoke Pollution / Q000009:adverse effects*; D014026:Nicotiana
D014028:Tobacco Smoke Pollution