In Silico Analysis Validates Proteomic Findings of Formalin-fixed Paraffin Embedded Cutaneous Squamous Cell Carcinoma Tissue.
BACKGROUND
Cutaneous squamous cell carcinoma (cSCC) is a common type of skin cancer but there are no comprehensive proteomic studies on this entity.


METHODS
We employed liquid chromatography coupled with tandem mass spectrometry (MS/MS) using formalin-fixed paraffin-embedded (FFPE) cSCC material to study the tumor and normal skin tissue proteomes. Ingenuity Pathway Analysis (IPA) was used to interpret the role of altered proteins in cSCC pathophysiology. Results were validated using the Human Protein Atlas and Oncomine database in silico.


RESULTS
Of 1,310 unique proteins identified, expression of an average of 144 and 88 proteins were significantly (p<0.05) increased and decreased, respectively, in the tumor samples compared to their normal counterparts. IPA analysis revealed disruptions in proteins associated with cell proliferation, apoptosis, and migration. In silico analysis confirmed that proteins corresponding to 12 antibodies, and genes corresponding to 18 proteins were differentially expressed between the two categories, validating our proteomic measurements.


CONCLUSIONS
Label-free MS-based proteomics is useful for analyzing FFPE cSCC tissues.
Department of Dermatology, Westmead Hospital, The University of Sydney, Westmead, NSW, Australia.
Ali
A
Azimi
School of Molecular Bioscience, Faculty of Science, The University of Sydney, Camperdown, NSW, Australia.|Brain and Mind Centre, The University of Sydney, Camperdown, NSW, Australia.
Kimberley L
KL
Kaufman
Department of Dermatology, Westmead Hospital, The University of Sydney, Westmead, NSW, Australia.
Marina
M
Ali
Dermatopathology, Skin and Cancer Foundation Australia, Darlinghurst, NSW, Australia.
Steven
S
Kossard
Department of Dermatology, Westmead Hospital, The University of Sydney, Westmead, NSW, Australia pablo.fernandezpenas@sydney.edu.au.
Pablo
P
Fernandez-Penas
D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000473:pathology; D002853:Chromatography, Liquid; D003198:Computer Simulation; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D009363:Neoplasm Proteins / Q000096:biosynthesis* / Q000235:genetics; D016612:Paraffin Embedding; D040901:Proteomics; D012878:Skin Neoplasms / Q000235:genetics* / Q000473:pathology; D053719:Tandem Mass Spectrometry; D016707:Tissue Fixation
D009363:Neoplasm Proteins
Cutaneous squamous cell carcinoma; formalin-fixed paraffin embedded; label-free mass spectrometry; laser capture microdissection; proteomics