Total flavonoid extract fromCoreopsis tinctoriaNutt. protects rats against myocardial ischemia/reperfusion injury.
OBJECTIVE
This study aimed to evaluate the protective effects of total flavonoid extract from Coreopsis tinctoria Nutt. (CTF) against myocardial ischemia/reperfusion injury (MIRI) using an isolated Langendorff rat heart model.


METHODS
Left ventricular developed pressure (LVDP) and the maximum rate of rise and fall of LV pressure (±dp/dtmax) were recorded. Cardiac injury was assessed by analyzing lactate dehydrogenase (LDH) and creatine kinase (CK) released in the coronary effluent. Superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), and malondialdehyde (MDA) levels were determined. Myocardial inflammation was assessed by monitoring tumor necrosis factor-alpha (TNF-α), C-reactive protein (CRP), interleukin-8 (IL-8), and interleukin-6 (IL-6) levels. Myocardial infarct size was estimated. Cell morphology was assessed by 2,3,5-triphenyltetrazolium chloride and hematoxylin and eosin (HE) staining. Cardiomyocyte apoptosis was determined by terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) staining.


RESULTS
Pretreatment with CTF significantly increased the heart rate and increased LVDP, as well as SOD and GSH-Px levels. In addition, CTF pretreatment decreased the TUNEL-positive cell ratio, infarct size, and levels of CK, LDH, MDA, TNF-α, CRP, IL-6, and IL-8.


CONCLUSIONS
These results suggest that CTF exerts cardio-protective effects against MIRI via anti-oxidant, anti-inflammatory, and anti-apoptotic activities.
Ministry of Education, Pharmacy Shihezi University Xinjiang China.
Ya
Y
Zhang
Ministry of Education, Pharmacy Shihezi University Xinjiang China.
Changsheng
C
Yuan
Ministry of Education, Pharmacy Shihezi University Xinjiang China.
He
H
Fang
Ministry of Education, Pharmacy Shihezi University Xinjiang China.
Jia
J
Li
Ministry of Education, Pharmacy Shihezi University Xinjiang China.
Shanshan
S
Su
Pharmacy Shihezi University Xinjiang China.
Wen
W
Chen
Anti-apoptosis; Anti-inflammatory; Anti-oxidant; Cardio-protective; Coreopsis tinctoria Nutt