Altered apoptosis/autophagy and epigenetic modifications cause the impaired postimplantation octaploid embryonic development in mice.
Polyploids are pervasive in plants and have large impacts on crop breeding, but natural polyploids are rare in animals. Mouse diploid embryos can be induced to become tetraploid by blastomere fusion at the 2-cell stage and tetraploid embryos can develop to the blastocyst stage in vitro. However, there is little information regarding mouse octaploid embryonic development and precise mechanisms contributing to octaploid embryonic developmental limitations are unknown. To investigate the genetic and epigenetic mechanisms underlying octaploid embryonic development, we generated mouse octaploid embryos and evaluated the in vitro/in vivo developmental potential. Here we show that octaploid embryos can develop to the blastocyst stage in vitro, but all fetus impaired immediately after implantation. Our results indicate that cell lineage specification of octaploid embryo was disorganized. Furthermore, these octaploid embryos showed increased apoptosis as well as alterations in epigenetic modifications when compared with diploid embryos. Thus, our cumulative data provide cues for why mouse octaploid embryonic development is limited and its failed postimplantation development.
a College of Animal Science and Technology, Nanjing Agricultural University , Nanjing , China.|b Research Center for Animal Genetic Resources of Mongolia Plateau, College of Life Science, Inner Mongolia University , Huhhot , China.|c Inner Mongolia Saikexing Institute of Breeding and Reproductive Biotechnology in Domestic Animal , Huhhot , China.
Bao-Jiang
BJ
Wu
b Research Center for Animal Genetic Resources of Mongolia Plateau, College of Life Science, Inner Mongolia University , Huhhot , China.|c Inner Mongolia Saikexing Institute of Breeding and Reproductive Biotechnology in Domestic Animal , Huhhot , China.
Li-Xia
LX
Zhao
a College of Animal Science and Technology, Nanjing Agricultural University , Nanjing , China.
Cheng-Cheng
CC
Zhu
b Research Center for Animal Genetic Resources of Mongolia Plateau, College of Life Science, Inner Mongolia University , Huhhot , China.
Yang-Lin
YL
Chen
b Research Center for Animal Genetic Resources of Mongolia Plateau, College of Life Science, Inner Mongolia University , Huhhot , China.
Meng-Yi
MY
Wei
b Research Center for Animal Genetic Resources of Mongolia Plateau, College of Life Science, Inner Mongolia University , Huhhot , China.|c Inner Mongolia Saikexing Institute of Breeding and Reproductive Biotechnology in Domestic Animal , Huhhot , China.
Si-Qin
SQ
Bao
a College of Animal Science and Technology, Nanjing Agricultural University , Nanjing , China.
Shao-Chen
SC
Sun
b Research Center for Animal Genetic Resources of Mongolia Plateau, College of Life Science, Inner Mongolia University , Huhhot , China.|c Inner Mongolia Saikexing Institute of Breeding and Reproductive Biotechnology in Domestic Animal , Huhhot , China.
Xi-He
XH
Li
D000818:Animals; D017209:Apoptosis / Q000235:genetics*; D001343:Autophagy / Q000235:genetics*; D015415:Biomarkers / Q000378:metabolism; D001755:Blastocyst / Q000166:cytology / Q000378:metabolism; D019070:Cell Lineage / Q000235:genetics; D004622:Embryo, Mammalian / Q000166:cytology / Q000378:metabolism; D047108:Embryonic Development / Q000235:genetics*; D044127:Epigenesis, Genetic; D005260:Female; D008813:Mice, Inbred ICR; D008954:Models, Biological; D011123:Polyploidy
D015415:Biomarkers
apoptosis; embryo development; epigenetics; octaploid