In vivo transgenic expression of collybistin in neurons of the rat cerebral cortex.
Collybistin (CB) is a guanine nucleotide exchange factor selectively localized to γ-aminobutyric acid (GABA)ergic and glycinergic postsynapses. Active CB interacts with gephyrin, inducing the submembranous clustering and the postsynaptic accumulation of gephyrin, which is a scaffold protein that recruits GABAAreceptors (GABAARs) at the postsynapse. CB is expressed with or without a src homology 3 (SH3) domain. We have previously reported the effects on GABAergic synapses of the acute overexpression of CBSH3-or CBSH3+in cultured hippocampal (HP) neurons. In the present communication, we are studying the effects on GABAergic synapses after chronic in vivo transgenic expression of CB2SH3-or CB2SH3+in neurons of the adult rat cerebral cortex. The embryonic precursors of these cortical neurons were in utero electroporated with CBSH3-or CBSH3+DNAs, migrated to the appropriate cortical layer, and became integrated in cortical circuits. The results show that: 1) the strength of inhibitory synapses in vivo can be enhanced by increasing the expression of CB in neurons; and 2) there are significant differences in the results between in vivo and in culture studies. J. Comp. Neurol. 525:1291-1311, 2017. © 2016 Wiley Periodicals, Inc.
Department of Physiology and Neurobiology, University of Connecticut, Storrs, Connecticut, 06269.
Christopher D
CD
Fekete
Department of Physiology and Neurobiology, University of Connecticut, Storrs, Connecticut, 06269.
Roman U
RU
Goz
Department of Physiology and Neurobiology, University of Connecticut, Storrs, Connecticut, 06269.
Sean
S
Dinallo
Department of Physiology and Neurobiology, University of Connecticut, Storrs, Connecticut, 06269.
Celia P
CP
Miralles
Department of Physiology and Neurobiology, University of Connecticut, Storrs, Connecticut, 06269.
Tzu-Ting
TT
Chiou
Department of Physiology and Neurobiology, University of Connecticut, Storrs, Connecticut, 06269.
John
J
Bear
Department of Physiology and Neurobiology, University of Connecticut, Storrs, Connecticut, 06269.
Christopher G
CG
Fiondella
Department of Physiology and Neurobiology, University of Connecticut, Storrs, Connecticut, 06269.
Joseph J
JJ
LoTurco
Department of Physiology and Neurobiology, University of Connecticut, Storrs, Connecticut, 06269.
Angel L
AL
De Blas
D000818:Animals; D002540:Cerebral Cortex / Q000254:growth & development / Q000378:metabolism*; D004622:Embryo, Mammalian; D005260:Female; D005455:Fluorescent Antibody Technique; D007091:Image Processing, Computer-Assisted; D008297:Male; D018613:Microscopy, Confocal; D055495:Neurogenesis / Q000502:physiology*; D009474:Neurons / Q000378:metabolism*; D018408:Patch-Clamp Techniques; D051381:Rats; D055647:Rats, Transgenic; D017208:Rats, Wistar; D064067:Rho Guanine Nucleotide Exchange Factors / Q000378:metabolism*; D013569:Synapses / Q000378:metabolism
C577404:Arhgef9 protein, rat; D064067:Rho Guanine Nucleotide Exchange Factors
GABAA receptors; GABAergic synapse; GABAergic synaptic plasticity; GABAergic synaptic strength; RRID: AB_10064220; RRID: AB_2232546; RRID: AB_2314482; RRID: AB_887873; RRID: RGD_2312511; RRID: SCR_002285; RRID: SCR_002815; RRID: SCR_002865; RRID: SCR_013674; RRID: SCR_014479; collybistin; gephyrin; in utero electroporation; synapse formation; vesicular GABA transporter