Genome-wide identification of ATP-binding cassette (ABC) transporters and conservation of their xenobiotic transporter function in the monogonont rotifer (Brachionus koreanus).
The ATP-binding cassette (ABC) transporter family is one of the largest gene family in animals, and members of this family are known to be involved in various biological processes due to their ability to transport a wide range of substrates across membranes using ATP cleavage-derived energy. We identified 61 ABC transporters in the genome of the monogonont rotifer Brachionus koreanus, and classified these into eight distinct subfamilies (A-H) by phylogenetic analysis. ABC transporters in the rotifer B. koreanus are comprised of 11 ABCA genes, 19 ABCB genes, 14 ABCC genes, 3 ABCD genes, 1 ABCE gene, 3 ABCF genes, 8 ABCG genes, and 2 ABCH genes. Extensive gene duplication and loss events in synteny were observed in several subfamilies. In particular, massive gene duplications of P-glycoproteins (P-gps), multidrug resistance proteins (MRPs), and Bk-Abcg-like proteins were observed. The ability of these B. koreanus proteins to function as multixenobiotic resistance (MXR) ABC transporters was validated using specific fluorescence substrates/inhibitors. The ABC transporter superfamily members identified in this study will be useful in future toxicological studies, and will facilitate comparative studies of the evolution of the ABC transporter superfamily in invertebrates.
Department of Biological Science, College of Science, Sungkyunkwan University, Suwon 16419, South Korea; Department of Chemistry, College of Natural Sciences, Hanyang University, Seoul 04763, South Korea.
Chang-Bum
CB
Jeong
Department of Biological Science, College of Science, Sungkyunkwan University, Suwon 16419, South Korea.
Hui-Su
HS
Kim
Department of Biological Science, College of Science, Sungkyunkwan University, Suwon 16419, South Korea.
Hye-Min
HM
Kang
Department of Biological Science, College of Science, Sungkyunkwan University, Suwon 16419, South Korea.
Young Hwan
YH
Lee
State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China.
Bingsheng
B
Zhou
Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Daejeon 34141, South Korea.
Joonho
J
Choe
Department of Biological Science, College of Science, Sungkyunkwan University, Suwon 16419, South Korea. Electronic address: jslee2@skku.edu.
Jae-Seong
JS
Lee
D018528:ATP-Binding Cassette Transporters / Q000235:genetics*; D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D002478:Cells, Cultured; D019295:Computational Biology; D020869:Gene Expression Profiling; D016678:Genome / Q000235:genetics*; D010802:Phylogeny; D012403:Rotifera / Q000187:drug effects / Q000235:genetics*; D015262:Xenobiotics / Q000494:pharmacology*
D018528:ATP-Binding Cassette Transporters; D015262:Xenobiotics; D000255:Adenosine Triphosphate
ABC transporter; Brachionus koreanus; MXR; Multixenobiotic resistance; Rotifer