Symmetry-mismatch reconstruction of genomes and associated proteins within icosahedral viruses using cryo-EM.
Although near-atomic resolutions have been routinely achieved for structural determination of many icosahedral viral capsids, structures of genomes and associated proteins within the capsids are still less characterized because the genome information is overlapped by the highly symmetric capsid information in the virus particle images. We recently developed a software package for symmetry-mismatch structural reconstruction and determined the structures of the genome and RNA polymerases within an icosahedral virus for the first time. Here, we describe the protocol used for this structural determination, which may facilitate structural biologists in investigating the structures of viral genome and associated proteins.
College of Physics and Information Science, Synergetic Innovation Center for Quantum Effects and Applications, Hunan Normal University, Changsha, 410081 China.
Xiaowu
X
Li
College of Physics and Information Science, Synergetic Innovation Center for Quantum Effects and Applications, Hunan Normal University, Changsha, 410081 China.
Hongrong
H
Liu
School of Life Sciences, Tsinghua University, Beijing, 100084 China.
Lingpeng
L
Cheng
Cryo-EM reconstruction; Symmetry-mismatch; Viral genome