X-Ray based Lung Function measurement-a sensitive technique to quantify lung function in allergic airway inflammation mouse models.
In mice, along with the assessment of eosinophils, lung function measurements, most commonly carried out by plethysmography, are essential to monitor the course of allergic airway inflammation, to examine therapy efficacy and to correlate animal with patient data. To date, plethysmography techniques either use intubation and/or restraining of the mice and are thus invasive, or are limited in their sensitivity. We present a novel unrestrained lung function method based on low-dose planar cinematic x-ray imaging (X-Ray Lung Function, XLF) and demonstrate its performance in monitoring OVA induced experimental allergic airway inflammation in mice and an improved assessment of the efficacy of the common treatment dexamethasone. We further show that XLF is more sensitive than unrestrained whole body plethysmography (UWBP) and that conventional broncho-alveolar lavage and histology provide only limited information of the efficacy of a treatment when compared to XLF. Our results highlight the fact that a multi-parametric imaging approach as delivered by XLF is needed to address the combined cellular, anatomical and functional effects that occur during the course of asthma and in response to therapy.
Institute for Diagnostic and Interventional Radiology, University Medical Center Goettingen, Germany.|Italian Synchrotron Light Source 'Elettra' Trieste, Italy.
C
C
Dullin
Max-Plank-Institute for Experimental Medicine, Dept. of Molecular Biology of Neuronal Signals, Goettingen, Germany.
M A
MA
Markus
Italian Synchrotron Light Source 'Elettra' Trieste, Italy.
E
E
Larsson
Italian Synchrotron Light Source 'Elettra' Trieste, Italy.
G
G
Tromba
Clinic for Anesthesiology, University Medical Center, Goettingen, Germany.
S
S
Hülsmann
Institute for Diagnostic and Interventional Radiology, University Medical Center Goettingen, Germany.|Max-Plank-Institute for Experimental Medicine, Dept. of Molecular Biology of Neuronal Signals, Goettingen, Germany.|Department of Hematology and Medical Oncology, University Medical Center, Goettingen, Germany.
F
F
Alves
D000818:Animals; D004195:Disease Models, Animal; D005260:Female; D006801:Humans; D007249:Inflammation / Q000175:diagnosis / Q000276:immunology / Q000503:physiopathology*; D008297:Male; D008807:Mice, Inbred BALB C; D010047:Ovalbumin / Q000276:immunology; D010993:Plethysmography, Whole Body / Q000379:methods; D015203:Reproducibility of Results; D012129:Respiratory Function Tests / Q000379:methods*; D012130:Respiratory Hypersensitivity / Q000175:diagnosis / Q000276:immunology / Q000503:physiopathology*; D012143:Respiratory Physiological Phenomena; D012680:Sensitivity and Specificity; D014965:X-Rays
D010047:Ovalbumin