Treatment of chemically induced oral ulcer using adipose-derived mesenchymal stem cell sheet.
BACKGROUND
This study investigated the effects of mesenchymal stem cell (MSC) sheet transplantation on healing of chemically induced oral ulceration in a rabbit animal model.


METHODS
Oral mucosal ulcers were induced by topical application of filter paper soaked with 70% acetic acid to the anterior gingiva and buccal mucosa of 12 New Zealand white rabbits. The animals were randomly assigned to two groups: with (treatment group, n = 6) or without (control group, n = 6) cell sheets applied to ulcers. Gross findings were sequentially evaluated, and histologic examination was performed on day 7.


RESULTS
Based on gross inspection, ulceration resolved before day 5 in the treatment group; however, in the control group, healing was incomplete on day 7. In the treatment group, the total area of the ulcer decreased significantly from day 2 to day 5 (P < 0.001) and from day 5 to day 7 (P = 0.020), whereas the area decreased significantly from day 5 to day 7 in the control group (P < 0.001). Histologic and immunofluorescence examination revealed full-thickness mucosa healing and complete basal cell coverage in the treatment group; in contrast, only partial healing was observed on day 7 in the control group.


CONCLUSIONS
Cell sheet technology using MSC can be an alternative treatment for oral ulcerations in that it can decrease healing time without invasive properties.
Department of Otorhinolaryngology-Head and Neck Surgery, Anam Hospital, Korea University Medical Center, Seoul, Korea.
Doh Young
DY
Lee
Department of Otorhinolaryngology-Head and Neck Surgery, Dongguk University Ilsan Hospital, Goyang, Korea.
Hee-Bok
HB
Kim
Asan Institute for Life Sciences, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Korea.
In Kyoung
IK
Shim
Institute of Advanced Biomedical Engineering and Science, TWIns, Tokyo Women's Medical University, Tokyo, Japan.
Nobuo
N
Kanai
Institute of Advanced Biomedical Engineering and Science, TWIns, Tokyo Women's Medical University, Tokyo, Japan.
Teruo
T
Okano
Department of Otorhinolaryngology-Head and Neck Surgery, Seoul National University Hospital, Seoul, Korea.|Cancer Research Institute, Seoul National University Hospital, Seoul, Korea.|Medical Research Center, Seoul National University Hospital, Seoul, Korea.
Seong Keun
SK
Kwon
D019342:Acetic Acid; D000273:Adipose Tissue / Q000166:cytology; D000818:Animals; D018929:Cell Culture Techniques; D004195:Disease Models, Animal; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells; D019226:Oral Ulcer / Q000139:chemically induced / Q000473:pathology / Q000503:physiopathology / Q000628:therapy*; D011817:Rabbits; D013997:Time Factors; D023822:Tissue Engineering / Q000379:methods; D014945:Wound Healing
D019342:Acetic Acid
cell sheet; mesenchymal stem cell; mucositis; oral ulcer