Effect of VEGF-C siRNA and endostatin on ring formation and proliferation of esophageal squamous cell carcinoma lymphatic endothelial cells.
OBJECTIVE
To study the effects of vascular endothelial growth factor C small interfering RNA and endostatin on esophageal squamous cell carcinoma-related ring formation in vitro and proliferation of lymphatic endothelial cells.


METHODS
KYSE150 cells were subjected to analysis of cell transfection and endostatin operation. The groups were as follows: negative group, blank group, negative plus endostatin group, endostatin group, SG1 group, SG2 group, SG1 plus endostatin group, and SG2 plus endostatin group. The esophageal cancer-related microlymphatic endothelial cells were three-dimensionally cultured. Cell Counting Kit-8 (CCK-8) assay was employed to detect cell proliferation.


RESULTS
The negative group's three-dimensional culture result was the highest, followed by the blank group, negative plus endostatin group, endostatin group, SG2 group, SG1 group, SG1 plus endostatin group, and SG2 plus endostatin group. The quantity of living cells in the blank group was the highest, followed by the negative control, endostatin, SG2, SG1, negative plus endostatin, SG1 plus endostatin, and SG2 plus endostatin groups.


CONCLUSIONS
Both vascular endothelial growth factor C small interfering RNA and endostatin could inhibit ring formation in esophageal squamous cell carcinoma and proliferation of lymphatic endothelial cells.
Pathology Department, The First Affiliated Hospital of Zhengzhou University; Henan Key Laboratory of Tumor Pathology; Pathology Department, The Second Hospital of Shandong University, Jinan.
Yuping
Y
Zheng
Pathology Department, Henan Tumor Hospital, Zhengzhou, People's Republic of China.
Miaomiao
M
Sun
Pathology Department, The First Affiliated Hospital of Zhengzhou University; Henan Key Laboratory of Tumor Pathology.
Jinyan
J
Chen
Pathology Department, The First Affiliated Hospital of Zhengzhou University; Henan Key Laboratory of Tumor Pathology.
Lulu
L
He
Pathology Department, The First Affiliated Hospital of Zhengzhou University; Henan Key Laboratory of Tumor Pathology.
Na
N
Zhao
Pathology Department, The First Affiliated Hospital of Zhengzhou University; Henan Key Laboratory of Tumor Pathology.
Kuisheng
K
Chen
VEGF-C; esophageal cancer-associated lymphatic endothelial cells; esophageal squamous carcinoma cells; proliferation; ring formation