Acute Response to Cigarette Smoking Assessed in Exhaled Breath Condensate in Patients with Chronic Obstructive Pulmonary Disease and Healthy Smokers.
The effect of acute exposure to cigarette smoke (CS) on the respiratory system has been less extensively studied than the long term effects of smoking. The aim of the present study was to evaluate the acute response to CS in smokers suffering from chronic obstructive pulmonary disease (COPD) and in healthy smokers. Nineteen stable COPD patients and 19 young healthy smokers were enrolled. Tumor necrosis factor alpha (TNF-α), IL-1β, and malondialdehyde (MDA) were measured in exhaled breath condensate (EBC) before and 60 min after smoking a cigarette. When pre- and post-CS levels of the evaluated biomarkers were compared, no differences were found in either group. However, the post-CS MDA was significantly greater in healthy smokers than that in COPD patients; 20.41 vs. 16.81 nmol/L, p = 0.01, respectively. Post-CS TNF-α correlated inversely with FEV1/FVC in healthy smokers. We conclude that CS does not acutely increase the EBC concentration of the inflammatory markers either in COPD patients or healthy smokers. The short term CS-induced oxidative stress is higher in young smokers than in COPD patients, which what may indicate a higher susceptibility to CS content of the former.
Department of Internal Medicine, Pneumology and Allergology, Medical University of Warsaw, 1A Banacha, 02-097, Warsaw, Poland.
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Maskey-Warzęchowska
Department of Internal Medicine, Pneumology and Allergology, Medical University of Warsaw, 1A Banacha, 02-097, Warsaw, Poland.
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Nejman-Gryz
Student Scientific Association 'Alveolus', Department of Internal Medicine, Pneumology and Allergology, Medical University of Warsaw, Warsaw, Poland.
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Osinka
Student Scientific Association 'Alveolus', Department of Internal Medicine, Pneumology and Allergology, Medical University of Warsaw, Warsaw, Poland.
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Lis
Student Scientific Association 'Alveolus', Department of Internal Medicine, Pneumology and Allergology, Medical University of Warsaw, Warsaw, Poland.
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Malesa
Department of Internal Medicine, Pneumology and Allergology, Medical University of Warsaw, 1A Banacha, 02-097, Warsaw, Poland. drkpgorska@gmail.com.
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Górska
Department of Internal Medicine, Pneumology and Allergology, Medical University of Warsaw, 1A Banacha, 02-097, Warsaw, Poland.
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Krenke
D000328:Adult; D000368:Aged; D015415:Biomarkers / Q000378:metabolism; D001944:Breath Tests / Q000379:methods; D045853:Exhalation / Q000502:physiology*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D018384:Oxidative Stress / Q000502:physiology; D029424:Pulmonary Disease, Chronic Obstructive / Q000378:metabolism / Q000503:physiopathology*; D012137:Respiratory System / Q000378:metabolism / Q000503:physiopathology; D012906:Smoke / Q000009:adverse effects*; D012907:Smoking / Q000009:adverse effects*; D014026:Nicotiana / Q000009:adverse effects*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism; D055815:Young Adult
D015415:Biomarkers; D012906:Smoke; D014409:Tumor Necrosis Factor-alpha
Airway obstruction; Inflammation; Malondialdehyde; Smoking; Tumor necrosis factor alpha