Determination of Alternate Splicing Events Using Transcriptome Arrays.
Understanding differential isoform expression is critical to mechanistically illuminate the biology underlying both normal development and disease states. High-throughput expression profiling analysis of splice variants has thus far been limited by sample requirements and an appropriate platform for quantitation and analysis. Here we describe Affymetrix GeneChip Human Transcriptome Array 2.0, which is employed for comprehensive examination of all known transcript isoforms.
Department of Biomedical Sciences, School of Public Health, Cancer Research Center, Rensselaer, NY, 12144, USA.
Rebecca S
RS
DeVaux
Center for Functional Genomics, University at Albany-SUNY, 1 Discovery Drive, Health Sciences Campus, Rensselaer, NY, 12144, USA.
Marcy
M
Kuentzel
Center for Functional Genomics, University at Albany-SUNY, 1 Discovery Drive, Health Sciences Campus, Rensselaer, NY, 12144, USA.
Jason
J
Herschkowitz
Department of Biomedical Sciences, School of Public Health, Cancer Research Center, Rensselaer, NY, 12144, USA. schittur@albany.edu.|Center for Functional Genomics, University at Albany-SUNY, 1 Discovery Drive, Health Sciences Campus, Rensselaer, NY, 12144, USA. schittur@albany.edu.
Sridar V
SV
Chittur
D017398:Alternative Splicing; D001483:Base Sequence; D045744:Cell Line, Tumor; D020869:Gene Expression Profiling; D006801:Humans; D012333:RNA, Messenger / Q000235:genetics* / Q000378:metabolism; D017423:Sequence Analysis, RNA; D059467:Transcriptome; D014584:User-Computer Interface
D012333:RNA, Messenger
Alternative splicing; Exon splicing; Expression profiling; Gene regulation; Microarray