Planomonospora algeriensis sp. nov., an actinobacterium isolated from a Saharan soil of Algeria.
A filamentous actinobacterium, designated strain PM3T, was isolated from a Saharan soil sample collected from Béni-Abbès, Béchar (South-West Algeria). A polyphasic taxonomic study was carried out to establish the status of strain PM3T. The isolate was found to have morphological and chemotaxonomical properties associated with members of the genus Planomonospora. The new isolated microorganism developed cylindrical sporangia arranged in double parallel rows on aerial mycelium, each one containing a motile single sporangiospore. The cell wall of the strain was found to contain meso-diaminopimelic acid. Whole-cell hydrolysates were found to contain madurose, glucose, mannose and ribose. The predominant menaquinone was identified as MK-9(H2) (69.6%). The polar lipids detected were identified as diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, phosphatidylethanolamine, phosphatidylhydroxyethanolamine and glucosamine-containing lipids. The major fatty acids were found to be C17:1ω9c (38.6%) and C17:0(24.2%). Results of 16S rRNA gene sequence comparison revealed that strain PM3Tshared a high degree of 16S rRNA gene sequence similarity with Planomonospora sphaerica DSM 44632T(99.3%), Planomonospora parontospora subsp. parontospora DSM 43177T(99.2%) and P. parontospora subsp. antibiotica DSM 43869T(99.0%). DNA-DNA hybridization values between strain PM3Tand the type strains of the closely related species were between 58.4 and 70.1%. The combination of phylogenetic analysis, DNA-DNA relatedness data, phenotypic characteristics and chemotaxonomic data support the conclusion that strain PM3Trepresents a novel species of the genus Planomonospora, for which the name Planomonospora algeriensis sp. nov. is proposed. The type strain is PM3T(=DSM 46752T= CECT 9047T).
Laboratoire de Biologie des Systèmes Microbiens (LBSM), Ecole Normale Supérieure de Kouba, Alger, Algeria.
Fawzia
F
Chaabane Chaouch
Laboratoire de Biologie des Systèmes Microbiens (LBSM), Ecole Normale Supérieure de Kouba, Alger, Algeria.|Département de Biologie, Faculté des Sciences de la Nature et de la Vie et Sciences de la Terre, Université de Ghardaïa, BP 455, 47000, Ghardaïa, Algeria.
Noureddine
N
Bouras
Laboratoire de Biologie des Systèmes Microbiens (LBSM), Ecole Normale Supérieure de Kouba, Alger, Algeria.
Salim
S
Mokrane
Laboratoire de Biologie des Systèmes Microbiens (LBSM), Ecole Normale Supérieure de Kouba, Alger, Algeria.
Khaoula
K
Bouznada
Laboratoire de Biologie des Systèmes Microbiens (LBSM), Ecole Normale Supérieure de Kouba, Alger, Algeria.
Abdelghani
A
Zitouni
Leibniz Institute DSMZ - German Collection of Microorganisms and Cell Cultures, Inhoffenstraße 7B, 38124, Braunschweig, Germany.
Gabriele
G
Pötter
Leibniz Institute DSMZ - German Collection of Microorganisms and Cell Cultures, Inhoffenstraße 7B, 38124, Braunschweig, Germany.
Cathrin
C
Spröer
School of Biology, Newcastle University, Ridley Building, Newcastle upon Tyne, NE1 7RU, UK. hans-peter.klenk@ncl.ac.uk.
Hans-Peter
HP
Klenk
Laboratoire de Biologie des Systèmes Microbiens (LBSM), Ecole Normale Supérieure de Kouba, Alger, Algeria. sabaou@yahoo.fr.
Nasserdine
N
Sabaou
D039903:Actinobacteria / Q000145:classification* / Q000235:genetics*; D000462:Algeria; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012988:Soil Microbiology
D012336:RNA, Ribosomal, 16S
Actinobacteria; Algerian Saharan soil; Planomonospora algeriensis sp. nov.; Polyphasic taxonomy