Cytotoxic Potential of Centaurea bruguierana ssp. belangerana: The MTT Assay.
The genus Centaurea with an extensive background in Iranian traditional medicine represents more than 70 species in Iran that some of them are endemic to Iran. A variety of secondary metabolites has been isolated from this genus thus far. Sesquiterpene lactones and flavonoids have been reported as main compounds of C.bruguierana. Methanolic extract and different fractions of the whole fruiting samples of Centaurea bruguierana ssp.belangerana (Asteraceae) were examined for cytotoxicity against various cell lines using MTT cytotoxicity assay in order to identify active fraction(s). The chloroform and ethyl acetate fractions of plant have demonstrated significant cytotoxicity against colon adenocarcinoma and breast ductal carcinoma cell lines, which chloroform fraction, exhibited the most potent in vitro cytotoxic activity against colon adenocarcinoma cell line, and therefore, can be considered as the potential fraction through main compounds of plant against the adenocarcinoma colon cancer cell line. Moreover, only chloroform fraction was moderately active against Swiss embryo fibroblast cell lines while the other fractions were nontoxic. To summarize, the chloroform fraction of C.bruguierana demonstrated better cytotoxic activity against all of the tested cell lines compared with the other fractions; and promisingly in some cases represented moderate to very good cytotoxicity that suggests more investigations about its phytochemical properties.
Department of Toxicology and Pharmacology, Poisoning Research Center, Tehran University of Medical Sciences, Tehran, Iran.
Seyed Nasser
SN
Ostad
Department of Pharmacognosy, Medicinal Plants Research Center, Tehran University of Medical Sciences, Tehran, Iran.
Afsaneh
A
Rajabi
Agricultural Research and Natural Resources, Bushehr, Iran.
Rahim
R
Khademi
Department of Traditional Pharmacy, School of Traditional Medicine, Tehran University of Medical Sciences, Tehran, Iran. AND Pharmaceutical Division, Food and Drug Organization, Ministry of Health and Medical Education, Tehran, Iran.
Fatemeh
F
Farjadmand
Department of Pharmacognosy, Medicinal Plants Research Center, Tehran University of Medical Sciences, Tehran, Iran.
Mahdieh
M
Eftekhari
Department of Pharmacognosy, Medicinal Plants Research Center, Tehran University of Medical Sciences, Tehran, Iran.
Abbas
A
Hadjiakhoondi
Department of Pharmacognosy, Medicinal Plants Research Center, Tehran University of Medical Sciences, Tehran, Iran. AND Faculty of Land and Food Systems, University of British Columbia, Vancouver, Canada.
Mahnaz
M
Khanavi
D000818:Animals; D045744:Cell Line, Tumor; D031179:Centaurea / Q000737:chemistry*; D005638:Fruit; D006801:Humans; D007492:Iran; D061986:MCF-7 Cells; D008519:Medicine, Traditional; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*
D010936:Plant Extracts
Asteraceae; Centaurea bruguierana ssp.belangerana; Cytotoxicity; IC50; MTT assay