A novel glucose sensor using lutetium phthalocyanine as redox mediator in reduced graphene oxide conducting polymer multifunctional hydrogel.
Herein, we report a scalable synthesis of multifunctional conducting polyacrylic acid (PAA) hydrogel (MFH) integrated with reduced grapheme oxide (rGO), vinyl substituted polyaniline (VS-PANI) and lutetium Phthalocyanine (LuPc2) as three dimensional robust matrix for glucose oxidase (GOx) immobilization (PAA-rGO/VS-PANI/LuPc2/GOx-MFH). We have integrated the multicomponents such as PAA with rGO, and VS-PANI through free radical polymerization using methylene bis-acrylamide, and ammonium persulphate as the cross linker and initiator. The LuPc2was then doped to form multifunctional hydrogel (PAA-rGO/VS-PANI/LuPc2-MFH). Finally, biosensor was fabricated by immobilizing GOx into PAA-rGO/VS-PANI/LuPc2-MFH and subsequently used for electrochemical detection of glucose. The PAA-rGO/VS-PANI/LuPc2/GOx-MFH biosensor exhibited high sensitivity (15.31μAmM-1cm-2) for the detection of glucose over a concentration range of 2-12mM with a low detection limit of 25µm. The PAA-rGO/VS-PANI/LuPc2-MFH biosensor showed a fast response time (1s) to the addition of glucose with high storage stability of 3 months. The real sample analysis reveals that PAA-rGO/VS-PANI/LuPc2/GOx-MFH could be effectively used as an electrochemical biosensor in industrial as well clinical diagnosis.
Materials and Engineering Research Institute, Sheffield Hallam University, Sheffield, UK.
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Al-Sagur
Materials and Engineering Research Institute, Sheffield Hallam University, Sheffield, UK.
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Komathi
Biomedical Research Institute, Sheffield Hallam University, Sheffield, UK.
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Khan
Gebze Technical University, Department of Chemistry, Gebze 41400, Kocaeli, Turkey.
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Gurek
Materials and Engineering Research Institute, Sheffield Hallam University, Sheffield, UK. Electronic address: aseel50.hassan@gmail.com.
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Hassan
D000814:Aniline Compounds / Q000737:chemistry; D015374:Biosensing Techniques / Q000379:methods*; D001786:Blood Glucose / Q000032:analysis*; D055664:Electrochemical Techniques / Q000379:methods; D004800:Enzymes, Immobilized / Q000737:chemistry; D005949:Glucose Oxidase / Q000737:chemistry; D006108:Graphite / Q000737:chemistry*; D006801:Humans; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000737:chemistry*; D007211:Indoles / Q000737:chemistry*; D054833:Isoindoles; D057230:Limit of Detection; D008187:Lutetium / Q000737:chemistry*; D010084:Oxidation-Reduction; D010087:Oxides / Q000737:chemistry
D000814:Aniline Compounds; D001786:Blood Glucose; D004800:Enzymes, Immobilized; D007211:Indoles; D054833:Isoindoles; D010087:Oxides; C416807:polyaniline; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D008187:Lutetium; D006108:Graphite; D005949:Glucose Oxidase; C013647:phthalocyanine
Glucose biosensor; Lutetium phthalocyanine; Multifunctional hydrogel; Polyacrylic acid; Polyaniline; Reduced graphene oxide