A two-step method for identification of the Chinese glutinous rice Suyunuo, based on ISSR-SCAR and allele-specific markers.
Suyunuo is a valuable glutinous rice variety cultivated mainly in the Lake Taihu area of China. Historically, Suyunuo was presented to emperors as a tribute, and, still today, enjoys a great reputation in China. This study aimed to develop a unique, specific molecular marker for the identification of Suyunuo rice. Polymerase chain reaction (PCR) amplification of inter-simple sequence repeat (ISSR) molecular markers was performed on Suyunuo and 11 other glutinous rice varieties that are mainly cultivated in the Yangtze River Delta region. A Suyunuo-specific band was detected in the PCR products generated from primer ISSR-807. A sequence characterized amplified region (SCAR) primer pair targeting a Suyunuo-specific band was subsequently designed. The SCAR primers amplified a target band in all individuals of Suyunuo and in four glutinous indica varieties, whereas no bands were found in the seven glutinous japonica varieties. Subsequently, sequences amplified by the SCAR primer pair were analyzed to facilitate the design of Suyunuo allele-specific primers. The allele-specific primer pair produced target bands in all individuals of Suyunuo rice but no bands in individuals of any of the other 11 rice varieties. This study provides a theoretical guideline for rice germplasm identification and innovation of other valuable rice landraces.
Seed Administrative Station of Suzhou, Suzhou, Jiangsu Province, China.
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Lin
Institute of Botany Jiangsu Province and Chinese Academy of Sciences, Nanjing, China.|The Jiangsu Provincial Platform for Conservation and Utilization of Agricultural Germplasm, Nanjing, China.
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Zhang
Institute of Botany Jiangsu Province and Chinese Academy of Sciences, Nanjing, China.|The Jiangsu Provincial Platform for Conservation and Utilization of Agricultural Germplasm, Nanjing, China.
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Hang
Institute of Botany Jiangsu Province and Chinese Academy of Sciences, Nanjing, China.|The Jiangsu Provincial Platform for Conservation and Utilization of Agricultural Germplasm, Nanjing, China.
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Li
Institute of Botany Jiangsu Province and Chinese Academy of Sciences, Nanjing, China.|The Jiangsu Provincial Platform for Conservation and Utilization of Agricultural Germplasm, Nanjing, China.
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Zhou
Seed Administrative Station of Suzhou, Suzhou, Jiangsu Province, China szseed@126.com.
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Shen
Institute of Botany Jiangsu Province and Chinese Academy of Sciences, Nanjing, China.|The Jiangsu Provincial Platform for Conservation and Utilization of Agricultural Germplasm, Nanjing, China.
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Sun
D000483:Alleles; D001483:Base Sequence; D017931:DNA Primers / Q000378:metabolism; D004587:Electrophoresis, Agar Gel; D005819:Genetic Markers; D018895:Microsatellite Repeats / Q000235:genetics*; D012275:Oryza / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods*; D015203:Reproducibility of Results; D016415:Sequence Alignment
D017931:DNA Primers; D005819:Genetic Markers