Design and Use of Chimeric Proteins Containing a Collagen-Binding Domain for Wound Healing and Bone Regeneration.
Collagen-based biomaterials are widely used in the field of tissue engineering; they can be loaded with biomolecules such as growth factors (GFs) to modulate the biological response of the host and thus improve its potential for regeneration. Recombinant chimeric GFs fused to a collagen-binding domain (CBD) have been reported to improve their bioavailability and the host response, especially when combined with an appropriate collagen-based biomaterial. This review first provides an extensive description of the various CBDs that have been fused to proteins, with a focus on the need for accurate characterization of their interaction with collagen. The second part of the review highlights the benefits of various CBD/GF fusion proteins that have been designed for wound healing and bone regeneration.
Biomedical Science and Technology Research Group, Bio-P2 Research Unit , Department of Chemical Engineering, École Polytechnique de Montréal, Montréal, Canada .
Cyril
C
Addi
Biomedical Science and Technology Research Group, Bio-P2 Research Unit , Department of Chemical Engineering, École Polytechnique de Montréal, Montréal, Canada .
Frederic
F
Murschel
Biomedical Science and Technology Research Group, Bio-P2 Research Unit , Department of Chemical Engineering, École Polytechnique de Montréal, Montréal, Canada .
Gregory
G
De Crescenzo
D000818:Animals; D001861:Bone Regeneration / Q000187:drug effects*; D003094:Collagen / Q000737:chemistry* / Q000494:pharmacology*; D006801:Humans; D000072417:Protein Domains; D015202:Protein Engineering / Q000379:methods*; D011994:Recombinant Proteins / Q000494:pharmacology*; D014945:Wound Healing / Q000187:drug effects*
D011994:Recombinant Proteins; D003094:Collagen
biomedical engineering; bone; collagen; extracellular matrix; growth factors; wound healing