Cloning and bioinformatic analysis of transcription factor MYB10 from the red-leaf peach.
In higher plants, the transcription factor MYB10 is an important regulator of anthocyanin biosynthesis. In order to study its role in the development of red coloration in peach leaves, the full-length MYB10 complementary DNA sequence of the red-leaf peach cultivar 'Tsukuba No. 5' (Prunus persica f. atropurpurea) was successfully cloned using reverse transcription-polymerase chain reaction. The sequence was assigned the GenBank accession No. KP315904. Bioinformatic analysis identified the complete MYB10 open reading frame, consisting of 678 bp encoding 225 amino acids. The predicted protein has a molecular weight of 26.56 kDa and a theoretical isoelectric point of 8.97. The secondary structure was found to comprise approximately 34.22% alpha helix, 15.11% extended strand, 10.67% beta turn, and 40% random coil. Subcellular analysis indicated that MYB10 may function in the cytoplasm. Assessment of the amino acid sequence suggested the presence of one serine and two threonine phosphorylation sites. Quantitative real-time polymerase chain reaction revealed that MYB10 expression positively correlated with anthocyanin content in red-leaf peach, indicating that this transcription factor plays a role in the biosynthesis of this pigment in peach trees.
College of Horticulture, Nanjing Agricultural University, Nanjing, China.
X Q
XQ
Wang
College of Horticulture, Nanjing Agricultural University, Nanjing, China.
J
J
Han
College of Horticulture, Nanjing Agricultural University, Nanjing, China.
Y
Y
Wen
College of Horticulture, Nanjing Agricultural University, Nanjing, China weibingj@sohu.com.
W B
WB
Jiang
College of Horticulture, Nanjing Agricultural University, Nanjing, China.
J G
JG
Fang
Institute of Horticulture, Jiangsu Academy of Agricultural Sciences/Jiangsu Key Laboratory of Horticultural Crop Genetic Improvement, Nanjing, China.
B B
BB
Zhang
Institute of Horticulture, Jiangsu Academy of Agricultural Sciences/Jiangsu Key Laboratory of Horticultural Crop Genetic Improvement, Nanjing, China weibingj@sohu.com.
R J
RJ
Ma
D000595:Amino Acid Sequence; D000872:Anthocyanins / Q000235:genetics* / Q000378:metabolism; D003001:Cloning, Molecular; D019295:Computational Biology; D018506:Gene Expression Regulation, Plant; D010858:Pigmentation; D018515:Plant Leaves / Q000235:genetics* / Q000378:metabolism; D010940:Plant Proteins / Q000096:biosynthesis / Q000235:genetics*; D000068239:Prunus persica / Q000235:genetics*
D000872:Anthocyanins; D010940:Plant Proteins