CRISPR/Cas9 in zebrafish: an efficient combination for human genetic diseases modeling.
The next-generation sequencing identifies a growing number of candidate genes associated with human genetic diseases, which inevitably requires efficient methods to validate the causal links between genotype and phenotype. Recently, zebrafish, with sufficiently high-throughput capabilities, has become a favored option to study human pathogenesis. In addition, CRISPR/Cas9-based approaches have radically reduced the efforts to introduce targeted genome engineering in various organisms. Here, we systemically review the basic considerations in the design of gene editing in zebrafish with CRISPR/Cas9, and explore the potential of the combination of these two to support efficient functional analysis of human genetic variants.
Department of Orthopaedic Surgery, Peking Union Medical College Hospital, Peking Union Medical College and Chinese Academy of Medical Sciences, No. 1 Shuaifuyuan, Beijing, 100730, China.|Beijing Key Laboratory for Genetic Research of Skeletal Deformity, Beijing, China.|Department of Breast Surgical Oncology, National Cancer Center/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.
Jiaqi
J
Liu
Beijing Key Laboratory for Genetic Research of Skeletal Deformity, Beijing, China.|Medical Research Center of Orthopaedics, Chinese Academy of Medical Sciences, Beijing, China.|Tsinghua University Medical School, Beijing, China.
Yangzhong
Y
Zhou
Department of General Surgery, Nanfang Hospital, Southern Medical University, Guangzhou, China.
Xiaolong
X
Qi
Department of Orthopaedic Surgery, Peking Union Medical College Hospital, Peking Union Medical College and Chinese Academy of Medical Sciences, No. 1 Shuaifuyuan, Beijing, 100730, China.|Beijing Key Laboratory for Genetic Research of Skeletal Deformity, Beijing, China.|Medical Research Center of Orthopaedics, Chinese Academy of Medical Sciences, Beijing, China.
Jia
J
Chen
Department of Orthopaedic Surgery, Peking Union Medical College Hospital, Peking Union Medical College and Chinese Academy of Medical Sciences, No. 1 Shuaifuyuan, Beijing, 100730, China.|Beijing Key Laboratory for Genetic Research of Skeletal Deformity, Beijing, China.|Medical Research Center of Orthopaedics, Chinese Academy of Medical Sciences, Beijing, China.
Weisheng
W
Chen
Department of Orthopaedic Surgery, Peking Union Medical College Hospital, Peking Union Medical College and Chinese Academy of Medical Sciences, No. 1 Shuaifuyuan, Beijing, 100730, China.|Beijing Key Laboratory for Genetic Research of Skeletal Deformity, Beijing, China.|Medical Research Center of Orthopaedics, Chinese Academy of Medical Sciences, Beijing, China.
Guixing
G
Qiu
Beijing Key Laboratory for Genetic Research of Skeletal Deformity, Beijing, China. orthoscience@126.com.|Medical Research Center of Orthopaedics, Chinese Academy of Medical Sciences, Beijing, China. orthoscience@126.com.|Department of Central Laboratory, Peking Union Medical College Hospital, Peking Union Medical College and Chinese Academy of Medical Sciences, No. 1 Shuaifuyuan, Beijing, 100730, China. orthoscience@126.com.
Zhihong
Z
Wu
Department of Orthopaedic Surgery, Peking Union Medical College Hospital, Peking Union Medical College and Chinese Academy of Medical Sciences, No. 1 Shuaifuyuan, Beijing, 100730, China. dr.wunan@pumch.cn.|Beijing Key Laboratory for Genetic Research of Skeletal Deformity, Beijing, China. dr.wunan@pumch.cn.|Medical Research Center of Orthopaedics, Chinese Academy of Medical Sciences, Beijing, China. dr.wunan@pumch.cn.
Nan
N
Wu
D000818:Animals; D064113:CRISPR-Cas Systems / Q000235:genetics*; D064112:Clustered Regularly Interspaced Short Palindromic Repeats / Q000235:genetics*; D004195:Disease Models, Animal; D000072669:Gene Editing; D005818:Genetic Engineering; D005826:Genetics, Medical; D015894:Genome, Human; D059014:High-Throughput Nucleotide Sequencing; D006801:Humans; D015027:Zebrafish / Q000235:genetics*