Serological study using glycoarray for detecting antibodies to glycolipids and glycolipid complexes in immune-mediated neuropathies.
We performed a serological investigation using glycoarray in Guillain-Barré syndrome (GBS), chronic inflammatory demyelinating polyradiculoneuropathy (CIDP), and multifocal motor neuropathy (MMN). Antibodies to 10 glycolipids and 45 glycolipid complexes were tested. Anti-GM1/sulfatide and anti-GA1/sulfatide IgG antibodies were common in GBS (20.0% and 19.0%, respectively). Anti-GQ1b/sulfatide IgG antibody was detected in 14.0% of GBS patients. IgG antibodies to antigens containing GQ1b were significantly correlated with ophthalmoplegia in GBS (p<0.01). IgM antibodies to antigens containing GM1 or GalNAc-GD1a were in 50% and 37.5% of MMN patients, respectively. Glycoarray is efficient for detecting antibodies against numerous glycolipid complexes in immune-mediated neuropathies.
Department of Neurology, Kindai University Faculty of Medicine, 377-2 Ohno-Higashi, Osaka-Sayama, Osaka 589-8511, Japan.
Miyuki
M
Morikawa
Department of Neurology, Kindai University Faculty of Medicine, 377-2 Ohno-Higashi, Osaka-Sayama, Osaka 589-8511, Japan.
Motoi
M
Kuwahara
Department of Neurology, Kindai University Faculty of Medicine, 377-2 Ohno-Higashi, Osaka-Sayama, Osaka 589-8511, Japan.
Rino
R
Ueno
Department of Neurology, Kindai University Faculty of Medicine, 377-2 Ohno-Higashi, Osaka-Sayama, Osaka 589-8511, Japan.
Makoto
M
Samukawa
Department of Neurology, Kindai University Faculty of Medicine, 377-2 Ohno-Higashi, Osaka-Sayama, Osaka 589-8511, Japan.
Yukihiro
Y
Hamada
Department of Neurology, Kindai University Faculty of Medicine, 377-2 Ohno-Higashi, Osaka-Sayama, Osaka 589-8511, Japan. Electronic address: kusunoki-tky@umin.ac.jp.
Susumu
S
Kusunoki
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001323:Autoantibodies / Q000097:blood*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005732:Gangliosides / Q000276:immunology; D006017:Glycolipids / Q000276:immunology*; D020275:Guillain-Barre Syndrome / Q000097:blood*; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood*; D007564:Japan; D008297:Male; D008875:Middle Aged; D011115:Polyneuropathies / Q000097:blood*; D020277:Polyradiculoneuropathy, Chronic Inflammatory Demyelinating / Q000097:blood*; D018709:Statistics, Nonparametric; D013433:Sulfoglycosphingolipids / Q000276:immunology; D055815:Young Adult
D001323:Autoantibodies; D005732:Gangliosides; D006017:Glycolipids; D007074:Immunoglobulin G; D013433:Sulfoglycosphingolipids; C039129:GQ1b ganglioside; C055253:IV(4)-galactosyl-N-acetylganglioside GD1a
Antibodies; Glycoarray; Glycolipid complex; Guillain-Barré syndrome; Multifocal motor neuropathy