MRZH reaction increases sensitivity for intrathecal IgG synthesis in IgG Oligoclonal band negative Multiple Sclerosis patients.
Given the low detection rates of CSF IgG-Oligoclonal bands (IgG-OCB) in non-European Multiple Sclerosis (MS) patients and higher specificity of the MRZH-reaction, we evaluated whether associating MRZH-reaction to CSF IgG-OCB detection improved investigation of suspected MS. Paired CSF and sera were analyzed for IgG-OCB and polyspecific viral antibodies. IgG-OCB were detected in 72% of MS patients and an MRZH-reaction in 67%. Combining IgG-OCB and MRZH raised detection of IgG abnormalities to 97% of studied MS patients. Detection of IgG-OCB and/or ≥2 MRZH antibodies showed sensitivity of 88% and specificity of 92% for MS, versus 72% and 96% for IgG-OCB alone.
Neuroimmunology Unit, University of Campinas, Campinas, Brazil; CATEM - Multiple Sclerosis Care Center, Santa Casa de São Paulo, São Paulo, Brazil. Electronic address: r.paterno@me.com.
Rafael P C
RP
Dias-Carneiro
Neuroimmunology Unit, University of Campinas, Campinas, Brazil; Department of Neurology, Brigham and Women's Hospital, Ann Romney Center for Neurologic Diseases, Harvard Medical School, Boston, USA. Electronic address: fvonglehnsilva@bwh.harvard.edu.
Felipe
F
von Glehn
Neuroimmunology Unit, University of Campinas, Campinas, Brazil. Electronic address: moraesadr@yahoo.com.br.
Adriel S
AS
Moraes
Neuroimmunology Unit, University of Campinas, Campinas, Brazil. Electronic address: vi_boldrini@hotmail.com.
Vinícius O
VO
Boldrini
Department of Neurology, University of Campinas, Campinas, Brazil. Electronic address: alfredodamasceno@hotmail.com.
Alfredo
A
Damasceno
Neuroimmunology Unit, University of Campinas, Campinas, Brazil. Electronic address: mdandrade43@gmail.com.
Marilia D
MD
Andrade
Neuroimmunology Unit, University of Campinas, Campinas, Brazil. Electronic address: allinydionete@yahoo.com.br.
Alliny C D
AC
Lima
Neuroimmunology Unit, University of Campinas, Campinas, Brazil. Electronic address: cristianecasanova@neurolife.com.br.
Cristiane S
CS
Casanova
CATEM - Multiple Sclerosis Care Center, Santa Casa de São Paulo, São Paulo, Brazil. Electronic address: charlespt@einstein.br.
Charles P
CP
Tilbery
Department of Neurology, University of Campinas, Campinas, Brazil. Electronic address: damascen@unicamp.br.
Benito P
BP
Damasceno
Neuroimmunology Unit, University of Campinas, Campinas, Brazil. Electronic address: leonilda@unicamp.br.
Leonilda Mb Dos
LM
Santos
Neuroimmunology Unit, University of Campinas, Campinas, Brazil; Department of Neurology, University of Campinas, Campinas, Brazil. Electronic address: carlosotavio@neurolife.com.br.
Carlos O
CO
Brandão
D000293:Adolescent; D000328:Adult; D000914:Antibodies, Viral / Q000134:cerebrospinal fluid*; D015415:Biomarkers / Q000134:cerebrospinal fluid; D005260:Female; D014645:Herpesvirus 3, Human / Q000378:metabolism; D006801:Humans; D007074:Immunoglobulin G / Q000096:biosynthesis* / Q000134:cerebrospinal fluid*; D008297:Male; D008459:Measles virus / Q000378:metabolism; D008875:Middle Aged; D009103:Multiple Sclerosis / Q000134:cerebrospinal fluid* / Q000175:diagnosis*; D043025:Oligoclonal Bands / Q000134:cerebrospinal fluid*; D012412:Rubella virus / Q000378:metabolism; D018139:Simplexvirus / Q000378:metabolism; D055815:Young Adult
D000914:Antibodies, Viral; D015415:Biomarkers; D007074:Immunoglobulin G; D043025:Oligoclonal Bands
Brazil; Cerebrospinal Fluid; Intrathecal synthesis; Multiple Sclerosis; Oligoclonal bands; Polyspecific antibodies