Possible involvement of iNOS and TNF-α in nutritional intervention against nicotine-induced pancreatic islet cell damage.
Nicotine is the more abundant and most significant components of cigarette smoke. Epidemiological evidence strongly suggests an association between cigarette smoking and pancreatic injury. Although effects of smoking on endocrine pancreas are still controversial Here, we examined the impact and underlying mechanisms of action of folic acid and vitamin B12 on nicotine induced damage in pancreatic islets of rats. Male Wistar rats were treated with nicotine (3mg/kg body weight/day, intraperitonealy) with or without folic acid (36μg/kg body weight/day, orally) and vitamin B12(0.63μg/kg body weight/day, orally) for 21days. Supplementation with folic acid and vitamin B12suppressed the nicotine induced changes in HbA1c, insulin, TNF-α, IL-6, generation of reactive oxygen species, and attenuated the changes in markers of oxidative stress. Moreover, folic acid and vitamin B12also counteracted the increased expression of protein and mRNA contents of TNF-α and iNOS produced by nicotine. Further, folic acid and vitamin B12in combination limits the nicotine induced changes in cell cycle and excessive apoptosis of the pancreatic β-cells and also successfully blunted the nicotine induced alteration in loss of mitochondrial membrane potential. In conclusion, data demonstrate that folic acid and vitamin B12may be possible nutritional intervention against cellular oxidative stress, which is a critical step in nicotine-mediated islet injury, and improves islet cell functional status by scavenging free radicals and by inhibiting the generation of pro-inflammatory mediators.
Department of Physiology, Serampore College, Serampore, Hooghly 712201, West Bengal, India.
Ankita
A
Bhattacharjee
Department of Physiology, Serampore College, Serampore, Hooghly 712201, West Bengal, India.
Shilpi Kumari
SK
Prasad
Department of Physiology, Y. S. Palpara Mahavidyalaya, Palpara, Purba Midnapore, West Bengal 721458, India.
Swagata
S
Pal
Department of Physiology, Serampore College, Serampore, Hooghly 712201, West Bengal, India.
Bithin
B
Maji
Department of Physiology, Serampore College, Serampore, Hooghly 712201, West Bengal, India.
Arnab
A
Banerjee
Department of Physiology, Serampore College, Serampore, Hooghly 712201, West Bengal, India.
Debasmita
D
Das
Department of Physiology, Serampore College, Serampore, Hooghly 712201, West Bengal, India.
Ananya
A
Bose
CSIR Indian Institute of Chemical Biology, 4, Raja S.C. Mullick Road, Kolkata 700032, India.
Nabanita
N
Chatterjee
Department of Physiology, Serampore College, Serampore, Hooghly 712201, West Bengal, India. Electronic address: sm_kdc@yahoo.co.in.
Sandip
S
Mukherjee
D000818:Animals; D000893:Anti-Inflammatory Agents / Q000008:administration & dosage*; D000975:Antioxidants / Q000008:administration & dosage*; D017209:Apoptosis / Q000187:drug effects; D015415:Biomarkers / Q000097:blood; D059447:Cell Cycle Checkpoints / Q000187:drug effects; D019610:Cytoprotection; D019587:Dietary Supplements; D004195:Disease Models, Animal; D005492:Folic Acid / Q000008:administration & dosage*; D005786:Gene Expression Regulation; D018836:Inflammation Mediators / Q000097:blood / Q000378:metabolism*; D007515:Islets of Langerhans / Q000187:drug effects* / Q000201:enzymology / Q000473:pathology; D008297:Male; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D009538:Nicotine; D052247:Nitric Oxide Synthase Type II / Q000235:genetics / Q000378:metabolism*; D018384:Oxidative Stress / Q000187:drug effects; D010182:Pancreatic Diseases / Q000139:chemically induced / Q000201:enzymology / Q000473:pathology / Q000517:prevention & control*; D017208:Rats, Wistar; D015398:Signal Transduction / Q000187:drug effects; D014409:Tumor Necrosis Factor-alpha / Q000097:blood / Q000235:genetics / Q000378:metabolism*; D014805:Vitamin B 12 / Q000008:administration & dosage*
D000893:Anti-Inflammatory Agents; D000975:Antioxidants; D015415:Biomarkers; D018836:Inflammation Mediators; D014409:Tumor Necrosis Factor-alpha; D009538:Nicotine; D005492:Folic Acid; D052247:Nitric Oxide Synthase Type II; C496318:Nos2 protein, rat; D014805:Vitamin B 12
Apoptosis; Islet cells; Oxidative stress; Pro-inflammatory mediators