A complete toolset for the study ofUstilago bromivoraandBrachypodiumsp. as a fungal-temperate grass pathosystem.
Due to their economic relevance, the study of plant pathogen interactions is of importance. However, elucidating these interactions and their underlying molecular mechanisms remains challenging since both host and pathogen need to be fully genetically accessible organisms. Here we present milestones in the establishment of a new biotrophic model pathosystem:Ustilago bromivoraandBrachypodiumsp. We provide a complete toolset, including an annotated fungal genome and methods for genetic manipulation of the fungus and its host plant. This toolset will enable researchers to easily study biotrophic interactions at the molecular level on both the pathogen and the host side. Moreover, our research on the fungal life cycle revealed a mating type bias phenomenon.U. bromivoraharbors a haplo-lethal allele that is linked to one mating type region. As a result, the identified mating type bias strongly promotes inbreeding, which we consider to be a potential speciation driver.
Gregor Mendel Institute, Austrian Academy of Sciences, Vienna Biocenter, Vienna, Austria.|Max Planck Institute for Terrestrial Microbiology, Marburg, Germany.
Franziska
F
Rabe
Gregor Mendel Institute, Austrian Academy of Sciences, Vienna Biocenter, Vienna, Austria.
Jason
J
Bosch
Gregor Mendel Institute, Austrian Academy of Sciences, Vienna Biocenter, Vienna, Austria.
Alexandra
A
Stirnberg
Gregor Mendel Institute, Austrian Academy of Sciences, Vienna Biocenter, Vienna, Austria.
Tilo
T
Guse
Gregor Mendel Institute, Austrian Academy of Sciences, Vienna Biocenter, Vienna, Austria.
Lisa
L
Bauer
Gregor Mendel Institute, Austrian Academy of Sciences, Vienna Biocenter, Vienna, Austria.
Denise
D
Seitner
Gregor Mendel Institute, Austrian Academy of Sciences, Vienna Biocenter, Vienna, Austria.
Fernando A
FA
Rabanal
Gregor Mendel Institute, Austrian Academy of Sciences, Vienna Biocenter, Vienna, Austria.
Angelika
A
Czedik-Eysenberg
Gregor Mendel Institute, Austrian Academy of Sciences, Vienna Biocenter, Vienna, Austria.
Simon
S
Uhse
Gregor Mendel Institute, Austrian Academy of Sciences, Vienna Biocenter, Vienna, Austria.
Janos
J
Bindics
Gregor Mendel Institute, Austrian Academy of Sciences, Vienna Biocenter, Vienna, Austria.
Bianca
B
Genenncher
Gregor Mendel Institute, Austrian Academy of Sciences, Vienna Biocenter, Vienna, Austria.
Fernando
F
Navarrete
Max Planck Institute for Plant Breeding Research, Cologne, Germany.
Ronny
0000-0002-4618-0110
R
Kellner
Vienna Biocenter Core Facilities GmbH, Vienna, Austria.
Heinz
H
Ekker
Leibniz-Institut für Pflanzengenetik und Kulturpflanzenforschung, Gatersleben, Germany.
Jochen
J
Kumlehn
DOE Joint Genome Institute, California, United States.
John P
JP
Vogel
DOE Joint Genome Institute, California, United States.
Sean P
SP
Gordon
INRA UMR BIOGER, AgroParisTech, Université Paris-Saclay, Thiverval-Grignon, France.
Thierry C
TC
Marcel
Institute of Bioinformatics and Systems Biology, Helmholtz Zentrum München, German Research Center for Environmental Health, Neuherberg, Germany.
Martin
M
Münsterkötter
Department of Genome-oriented Bioinformatics, Wissenschaftszentrum Weihenstephan, Technische Universität München, Freising, Germany.
Mathias C
0000-0003-3012-2626
MC
Walter
Institute of Bioinformatics and Systems Biology, Helmholtz Zentrum München, German Research Center for Environmental Health, Neuherberg, Germany.
Christian Mk
CM
Sieber
Max Planck Institute for Terrestrial Microbiology, Marburg, Germany.|Institute of Bioinformatics and Systems Biology, Helmholtz Zentrum München, German Research Center for Environmental Health, Neuherberg, Germany.
Gertrud
G
Mannhaupt
Institute of Bioinformatics and Systems Biology, Helmholtz Zentrum München, German Research Center for Environmental Health, Neuherberg, Germany.|Department of Genome-oriented Bioinformatics, Wissenschaftszentrum Weihenstephan, Technische Universität München, Freising, Germany.
Ulrich
0000-0001-5052-8610
U
Güldener
Max Planck Institute for Terrestrial Microbiology, Marburg, Germany.
Regine
R
Kahmann
Gregor Mendel Institute, Austrian Academy of Sciences, Vienna Biocenter, Vienna, Austria.|Max Planck Institute for Terrestrial Microbiology, Marburg, Germany.
Armin
0000-0002-8087-9566
A
Djamei
D058431:Brachypodium / Q000235:genetics* / Q000382:microbiology*; D049770:Genes, Mating Type, Fungal; D054884:Host-Pathogen Interactions; D008967:Molecular Biology / Q000379:methods*; D014588:Ustilago / Q000235:genetics* / Q000502:physiology*
Brachypodium distachyon; Ustilago bromivora; biotrophic interaction; effector; grass; infectious disease; microbiology; plant biology; plant pathogen