A proteomic analysis of acute leukemia cells treated with 9-hydroxyoctadecadienoic acid.
BACKGROUND
9s-hydroxy-octadecadienoic acid (9S-HOD), one of the natural products of linoleic acid oxygenation by 15-lipoxygenase (15-LOX), has been found to have anti-tumor properties in vitro and in vivo. The present study therefore investigated whether 9S-HOD affects acute leukemia HL-60 cells.


METHODS
The cytotoxicity of 9S-HOD in HL-60 with or without the presence of fetal bovine serum (FBS) in the culture media was tested using cell viability assays and flow cytometry. To explore the mechanism of its anti-tumor activity by 9S-HOD, we used a proteomic analysis to identify HL-60 cells protein profiles, based on two-dimensional gel electrophoresis (2-DE) and mass spectrometry (MS) identification.


RESULTS
9S-HOD exerted cytotoxicity efficacy and induced apoptosis in HL-60 cells, and the cytotoxicity was largely attenuated by the presence of FBS in culture media. The proteomic results revealed that 9S-HOD remarkably altered the abundance of 23 proteins that were involved in mRNA metabolic process, protein binding, DNA replication and apoptosis.


CONCLUSIONS
Our results indicated that 9S-HOD exerts cytotoxicity in HL-60 cells by affecting several pathways.
School of Laboratory Medicine and Biotechnology, Southern Medical University, Guangzhou, Guangdong Province, 510515, People's Republic of China.
Zhen
Z
Li
School of Laboratory Medicine and Biotechnology, Southern Medical University, Guangzhou, Guangdong Province, 510515, People's Republic of China.
Bohong
B
Chen
School of Laboratory Medicine and Biotechnology, Southern Medical University, Guangzhou, Guangdong Province, 510515, People's Republic of China.
Ping
P
Wang
School of Laboratory Medicine and Biotechnology, Southern Medical University, Guangzhou, Guangdong Province, 510515, People's Republic of China.
Xin
X
Li
School of Laboratory Medicine and Biotechnology, Southern Medical University, Guangzhou, Guangdong Province, 510515, People's Republic of China.
Gaotai
G
Cai
School of Laboratory Medicine and Biotechnology, Southern Medical University, Guangzhou, Guangdong Province, 510515, People's Republic of China.
Wei
W
Wei
School of Laboratory Medicine and Biotechnology, Southern Medical University, Guangzhou, Guangdong Province, 510515, People's Republic of China. dongwq63@263.net.
Wenqi
W
Dong
D000970:Antineoplastic Agents / Q000494:pharmacology* / Q000627:therapeutic use; D017209:Apoptosis / Q000187:drug effects*; D005231:Fatty Acids, Unsaturated / Q000494:pharmacology* / Q000627:therapeutic use; D018922:HL-60 Cells; D006801:Humans; D007938:Leukemia / Q000188:drug therapy* / Q000378:metabolism; D020543:Proteome / Q000187:drug effects*
C000616972:9-hydroxyoctadecadienoic acid; D000970:Antineoplastic Agents; D005231:Fatty Acids, Unsaturated; D020543:Proteome
9s-hydroxy-octadecadienoic acid; Acute leukemia cells; Bioinformatics analysis; Proteomics