CD40 functional gene polymorphisms and mRNA expression in rheumatoid arthritis patients from western Mexico.
The CD40 pathway is involved in the development and pathogenesis of autoimmune diseases, including rheumatoid arthritis (RA). Two single nucleotide polymorphisms (SNPs) in the CD40 gene, rs1883832 and rs4810485, are associated with susceptibility to inflammatory and autoimmune diseases and are thought to alter CD40 expression at the mRNA and protein level. This study assessed for the first time the association of these SNPs with RA and CD40 mRNA levels in a western Mexican population. A total of 278 RA patients and 318 control subjects were included. Genotyping was performed by polymerase chain reaction (PCR)-restriction fragment length polymorphism, and CD40 mRNA expression was determined by real-time quantitative PCR. No significant differences in genotype and allele frequencies were identified between the RA patients and controls. When stratified by genotype, these SNPs were not found to be associated with the presence of autoantibodies or the clinical activity of the disease. CD40 mRNA levels were elevated 1.5-fold in RA patients compared to control subjects; however, no clear tendencies were observed following stratification by genotype. These results suggest that the CD40 SNPs rs1883832 and rs4810485 are not RA susceptibility markers in the western Mexican population. Further studies are needed to clarify their roles in CD40 mRNA expression.
Research Institute in Biomedical Sciences, CUCS, University of Guadalajara, Guadalajara, Jalisco, Mexico.
I V
IV
Román-Fernández
Research Institute in Biomedical Sciences, CUCS, University of Guadalajara, Guadalajara, Jalisco, Mexico.
D F
DF
Ávila-Castillo
Division of Rheumatology, Guadalajara Civil Hospital "Fray Antonio Alcalde", Guadalajara, Jalisco, Mexico.
S
S
Cerpa-Cruz
Division of Rheumatology, Guadalajara Civil Hospital "Fray Antonio Alcalde", Guadalajara, Jalisco, Mexico.
S
S
Gutiérrez-Ureña
Research Institute in Biomedical Sciences, CUCS, University of Guadalajara, Guadalajara, Jalisco, Mexico.
J
J
Hernández-Bello
Research Institute in Biomedical Sciences, CUCS, University of Guadalajara, Guadalajara, Jalisco, Mexico.
J R
JR
Padilla-Gutiérrez
Research Institute in Biomedical Sciences, CUCS, University of Guadalajara, Guadalajara, Jalisco, Mexico.
Y
Y
Valle
Laboratory of Immunology, CUCS, University of Guadalajara, Guadalajara, Jalisco, Mexico.
M G
MG
Ramírez-Dueñas
Laboratory of Immunology, CUCS, University of Guadalajara, Guadalajara, Jalisco, Mexico.
A L
AL
Pereira-Suárez
Research Institute in Biomedical Sciences, CUCS, University of Guadalajara, Guadalajara, Jalisco, Mexico biologiamolecular@hotmail.com.
J F
JF
Muñoz-Valle
D000328:Adult; D000368:Aged; D001172:Arthritis, Rheumatoid / Q000235:genetics* / Q000473:pathology; D019013:CD40 Antigens / Q000096:biosynthesis / Q000235:genetics*; D005260:Female; D005786:Gene Expression Regulation; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D008297:Male; D008800:Mexico; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics
D019013:CD40 Antigens; D012333:RNA, Messenger