Transcriptomic Variation during Spermiogenesis in Mouse Germ Cells.
To explore variations in the transcription activity during spermiogenesis, round and elongated spermatids were collected from ICR/CD1 model mice using laser capture microdissection (LCM) and cauda epididymal sperm samples. The transcripts were sequenced using RNA-seq, and the reads were mapped to mm9. The majority of the reads (70%) in the round and elongated spermatids were mappable to known and predicted exons, but that in sperm was only 9%. The results of the distribution of reads suggested that alternative splicing was more complicated in sperm than in round and elongated spermatids. In the 19,127 genes, we detected the expression of 5,104 de novo genes and 91,112 alternative splicing events, and 12,105 were differentially expressed. Gene ontology (GO), InterPro domains, and KEGG revealed changes in gene transcription, mitochondrial protein translation, cellular components, and energy metabolism during spermiogenesis. The results provided considerable information about alternative splicing events, differentiallly expressed genes (DEGs), and novel transcriptions during spermiogenesis in mice.
The Key Laboratory for Farm Animal Genetic and Utilization of Ministry of Agriculture of China, Institute of Animal Science, Chinese Academy of Agricultural Science, Beijing, 100193, China.|JiLin Agriculture University Animal Science and Technology, Changchun, 130118, China.
Haiyang
H
Zuo
The Key Laboratory for Farm Animal Genetic and Utilization of Ministry of Agriculture of China, Institute of Animal Science, Chinese Academy of Agricultural Science, Beijing, 100193, China.
Junfang
J
Zhang
The Key Laboratory for Farm Animal Genetic and Utilization of Ministry of Agriculture of China, Institute of Animal Science, Chinese Academy of Agricultural Science, Beijing, 100193, China.|HaiKou Mary Hospital Reproductive Medicine Center, Haikou, 570100, China.
Liuguang
L
Zhang
The Key Laboratory for Farm Animal Genetic and Utilization of Ministry of Agriculture of China, Institute of Animal Science, Chinese Academy of Agricultural Science, Beijing, 100193, China.
Xiaoxia
X
Ren
The Key Laboratory for Farm Animal Genetic and Utilization of Ministry of Agriculture of China, Institute of Animal Science, Chinese Academy of Agricultural Science, Beijing, 100193, China.
Xiaoli
X
Chen
The Key Laboratory for Farm Animal Genetic and Utilization of Ministry of Agriculture of China, Institute of Animal Science, Chinese Academy of Agricultural Science, Beijing, 100193, China.
Haisheng
H
Hao
The Key Laboratory for Farm Animal Genetic and Utilization of Ministry of Agriculture of China, Institute of Animal Science, Chinese Academy of Agricultural Science, Beijing, 100193, China.
Xueming
X
Zhao
The Key Laboratory for Farm Animal Genetic and Utilization of Ministry of Agriculture of China, Institute of Animal Science, Chinese Academy of Agricultural Science, Beijing, 100193, China.
Dong
D
Wang
D017398:Alternative Splicing; D000818:Animals; D004734:Energy Metabolism / Q000235:genetics; D063990:Gene Ontology; D014644:Genetic Variation; D061346:Laser Capture Microdissection; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D024101:Mitochondrial Proteins / Q000235:genetics* / Q000378:metabolism; D058977:Molecular Sequence Annotation; D017423:Sequence Analysis, RNA; D013087:Spermatids / Q000254:growth & development / Q000378:metabolism* / Q000648:ultrastructure; D013091:Spermatogenesis; D013094:Spermatozoa / Q000254:growth & development / Q000378:metabolism* / Q000648:ultrastructure; D059467:Transcriptome
D024101:Mitochondrial Proteins