Magneto-controlled aptasensor for simultaneous electrochemical detection of dual mycotoxins in maize using metal sulfide quantum dots coated silica as labels.
Currently there is an urgent need for multi-mycotoxin detection methods due to the co-occurrence of multiple mycotoxins in food raw materials and their augmented toxicity. Herein, a magneto-controlled aptasensor has been developed for simultaneous electrochemical detection of ochratoxin A (OTA) and fumonisin B1 (FB1), two typical mycotoxins found in food crops world-wide. This aptasensor was designed using the high specificity between the target and aptamer with heavy CdTe or PbS quantum dots (QDs) coated silica as labels and the complementary DNA functionalized magnetic beads as capture probes. In presence of targets, the aptamer preferred to form the target-aptamer binding which forced the partial release of the preloaded labels from the magnetic beads. After a one-step incubation and a simple magnetic separation, the electrochemical signals of Cd2+and Pb2+dissolved from the reserved labels which had negative correlation with targets contents, was measured based on the difference of peak potentials. This aptasensor provided a wide detection range of 10pgmL-1to 10ngmL-1for OTA and 50pgmL-1to 50ngmL-1for FB1, and succeeded in real maize samples. This method provides a new avenue for high throughput screen of mycotoxins due to the advantages of simple instrument, low sample consumption, short assay times, and lower detection costs per assay. Moreover, it could be readily expanded for the simultaneous detection of a large panel of mycotoxins by using different metal sulfide QDs when their specific aptamers are available.
School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China; Changzhou College of Information Technology, Changzhou 213164, China.
Chengquan
C
Wang
Key Laboratory of Modern Agriculture Equipment and Technology, School of Chemistry and Chemical Engineering, Jiangsu University, Zhenjiang 212013, China.
Jing
J
Qian
Key Laboratory of Modern Agriculture Equipment and Technology, School of Chemistry and Chemical Engineering, Jiangsu University, Zhenjiang 212013, China.
Keqi
K
An
School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China. Electronic address: h_xingyi@163.com.
Xingyi
X
Huang
Key Laboratory of Modern Agriculture Equipment and Technology, School of Chemistry and Chemical Engineering, Jiangsu University, Zhenjiang 212013, China.
Lufang
L
Zhao
Key Laboratory of Modern Agriculture Equipment and Technology, School of Chemistry and Chemical Engineering, Jiangsu University, Zhenjiang 212013, China.
Qian
Q
Liu
Key Laboratory of Modern Agriculture Equipment and Technology, School of Chemistry and Chemical Engineering, Jiangsu University, Zhenjiang 212013, China.
Nan
N
Hao
Key Laboratory of Modern Agriculture Equipment and Technology, School of Chemistry and Chemical Engineering, Jiangsu University, Zhenjiang 212013, China. Electronic address: wangkun@ujs.edu.cn.
Kun
K
Wang
D052157:Aptamers, Nucleotide / Q000737:chemistry*; D015374:Biosensing Techniques / Q000379:methods*; D019187:Cadmium Compounds / Q000737:chemistry; D055664:Electrochemical Techniques / Q000379:methods; D005504:Food Analysis / Q000379:methods; D005506:Food Contamination / Q000032:analysis; D037341:Fumonisins / Q000032:analysis*; D007854:Lead / Q000737:chemistry; D057230:Limit of Detection; D008280:Magnetics / Q000379:methods; D059346:Magnets / Q000737:chemistry; D009793:Ochratoxins / Q000032:analysis*; D045663:Quantum Dots / Q000737:chemistry* / Q000648:ultrastructure; D012822:Silicon Dioxide / Q000737:chemistry; D013440:Sulfides / Q000737:chemistry; D013691:Tellurium / Q000737:chemistry; D003313:Zea mays / Q000737:chemistry*
D052157:Aptamers, Nucleotide; D019187:Cadmium Compounds; D037341:Fumonisins; D009793:Ochratoxins; D013440:Sulfides; C025589:ochratoxin A; C018391:lead sulfide; D007854:Lead; C056933:fumonisin B1; D012822:Silicon Dioxide; D013691:Tellurium; C028337:cadmium telluride
Electrochemical aptasensor; Fumonisin B1; Magneto-controlled; Mycotoxins; Ochratoxin A; Simultaneous detection