Metformin Reduces Lipogenesis Markers in Obese Mice Fed a Low-Carbohydrate and High-Fat Diet.
Lipogenesis is the process by which fatty acids are synthesized. In metabolic syndrome, an insulin resistant state along with high plasma levels of free fatty acids (FFA) and hyperglycemia may contribute to the lipogenic process. The aim of the present study was to investigate the effects of oral administration of metformin on the expression of lipogenic genes and glycemic profile in mice fed with low-carbohydrate high-fat diet by evaluating their metabolic profile. SWISS male mice were divided into 4 groups (N = 7) that were fed with standard (ST), standard plus metformin (ST + MET), low-carbohydrate high-fat diet (LCHFD) and low-carbohydrate high-fat diet plus metformin (LCHFD + MET) (100 mg kg-1diet) diets respectively. Food intake, body weight and blood parameters, such as glucose tolerance, insulin sensitivity, glucose, HDL-c, total cholesterol, triglycerides, ASL and ALT levels were assessed. Histological analyses were performed on hematoxylin and eosin-stained epididymal adipose tissue histological specimens. The expression levels of peroxisome proliferator-activated receptor (PPARγ), sterol regulatory element-binding protein 1 (SREBP1), fatty acid synthase (FAS) and acetyl-CoA carboxylase (ACC), were assessed by RT-PCR. This study showed that metformin decreased adipocyte area, body weight and food consumption in obese animals when compared to the standard group. Furthermore, the expression of lipogenic markers in adipose tissue were diminished in obese animals treated with metformin. This data showed that oral administration of metformin improved glucose and lipid metabolic parameters in white adipose tissue by reducing the expression of lipogenesis markers, suggesting an important clinical application of MET in treating obesity-related diseases in metabolic syndrome.
Laboratory of Health Science, Postgraduate Program in Health Sciences, Universidade Estadual de Montes Claros (Unimontes), Montes Claros, MG, Brazil.
Karla Nayara
KN
de Oliveira Santana
Laboratory of Health Science, Postgraduate Program in Health Sciences, Universidade Estadual de Montes Claros (Unimontes), Montes Claros, MG, Brazil.
Deborah Farias
DF
Lelis
Laboratory of Health Science, Postgraduate Program in Health Sciences, Universidade Estadual de Montes Claros (Unimontes), Montes Claros, MG, Brazil.
Keila Lopes
KL
Mendes
Laboratory of Health Science, Postgraduate Program in Health Sciences, Universidade Estadual de Montes Claros (Unimontes), Montes Claros, MG, Brazil.
Jamille Fernandes
JF
Lula
Laboratory of Health Science, Postgraduate Program in Health Sciences, Universidade Estadual de Montes Claros (Unimontes), Montes Claros, MG, Brazil.
Alanna Fernandes
AF
Paraíso
Laboratory of Health Science, Postgraduate Program in Health Sciences, Universidade Estadual de Montes Claros (Unimontes), Montes Claros, MG, Brazil.
João Marcus Oliveira
JM
Andrade
Texas Tech University, Lubbock, TX, USA.
John David
JD
Feltenberger
Institute of Agricultural Sciences, Food Engineering College, Universidade Federal de Minas Gerais (UFMG), Avenida Universitária, 1.000-Universitário, Montes Claros, MG, 39404-547, Brazil.
Junio
J
Cota
Institute of Agricultural Sciences, Food Engineering College, Universidade Federal de Minas Gerais (UFMG), Avenida Universitária, 1.000-Universitário, Montes Claros, MG, 39404-547, Brazil.
Diego Vicente
DV
da Costa
Laboratory of Health Science, Postgraduate Program in Health Sciences, Universidade Estadual de Montes Claros (Unimontes), Montes Claros, MG, Brazil.|Department of Dentistry, Universidade Estadual de Montes Claros (Unimontes), Montes Claros, MG, Brazil.
Alfredo Mauricio Batista
AM
de Paula
Laboratory of Health Science, Postgraduate Program in Health Sciences, Universidade Estadual de Montes Claros (Unimontes), Montes Claros, MG, Brazil.|Department of Dentistry, Universidade Estadual de Montes Claros (Unimontes), Montes Claros, MG, Brazil.
André Luiz Sena
AL
Guimarães
Laboratory of Health Science, Postgraduate Program in Health Sciences, Universidade Estadual de Montes Claros (Unimontes), Montes Claros, MG, Brazil. sergiosousas@hotmail.com.|Institute of Agricultural Sciences, Food Engineering College, Universidade Federal de Minas Gerais (UFMG), Avenida Universitária, 1.000-Universitário, Montes Claros, MG, 39404-547, Brazil. sergiosousas@hotmail.com.
Sérgio Henrique Sousa
SH
Santos
D000103:Acetyl-CoA Carboxylase / Q000235:genetics; D000273:Adipose Tissue / Q000187:drug effects / Q000378:metabolism; D000284:Administration, Oral; D000818:Animals; D015415:Biomarkers / Q000097:blood*; D001835:Body Weight / Q000187:drug effects; D050528:Diet, Carbohydrate-Restricted; D059305:Diet, High-Fat; D004435:Eating / Q000187:drug effects; D064429:Fatty Acid Synthases / Q000235:genetics; D005786:Gene Expression Regulation / Q000187:drug effects; D007004:Hypoglycemic Agents / Q000008:administration & dosage* / Q000494:pharmacology; D050155:Lipogenesis / Q000187:drug effects*; D008297:Male; D008687:Metformin / Q000008:administration & dosage* / Q000494:pharmacology; D051379:Mice; D008820:Mice, Obese; D009765:Obesity / Q000235:genetics / Q000378:metabolism*; D047495:PPAR gamma / Q000235:genetics; D051780:Sterol Regulatory Element Binding Protein 1 / Q000235:genetics
D015415:Biomarkers; D007004:Hypoglycemic Agents; D047495:PPAR gamma; C497388:Srebf1 protein, mouse; D051780:Sterol Regulatory Element Binding Protein 1; D008687:Metformin; D064429:Fatty Acid Synthases; D000103:Acetyl-CoA Carboxylase
Adipose tissue; Lipogenesis; Metabolism; Metformin; Obesity