A rapid, automated surface protein profiling of single circulating exosomes in human blood.
Circulating exosomes provide a promising approach to assess novel and dynamic biomarkers in human disease, due to their stability, accessibility and representation of molecules from source cells. However, this potential has been stymied by lack of approaches for molecular profiling of individual exosomes, which have a diameter of 30-150 nm. Here we report a rapid analysis approach to evaluate heterogeneous surface protein expression in single circulating exosomes from human blood. Our studies show a differential CD47 expression in blood-derived individual circulating exosomes that is correlated with breast cancer status, demonstrating a great potential of individual exosome profiles in biomarker discovery. The sensitive and high throughput platform of single exosome analysis can also be applied to characterizing exosomes derived from other patient fluids.
Department of Pathology, School of Medicine, Case Western Reserve University, Cleveland, Ohio, USA.
Golam
G
Kibria
Department of Pathology, School of Medicine, Case Western Reserve University, Cleveland, Ohio, USA.
Erika K
EK
Ramos
Department of Pathology, School of Medicine, Case Western Reserve University, Cleveland, Ohio, USA.
Katelyn E
KE
Lee
Department of Pathology, School of Medicine, Case Western Reserve University, Cleveland, Ohio, USA.
Sarah
S
Bedoyan
Department of Pathology, School of Medicine, Case Western Reserve University, Cleveland, Ohio, USA.
Simo
S
Huang
The Institute Molecular Engineering, the University of Chicago, Chicago, Illinois, USA.
Ravand
R
Samaeekia
Department of Pathology, School of Medicine, Case Western Reserve University, Cleveland, Ohio, USA.
Jaffre J
JJ
Athman
Department of Pathology, School of Medicine, Case Western Reserve University, Cleveland, Ohio, USA.
Clifford V
CV
Harding
Krefting Research Centre, Department of Internal Medicine and Clinical Nutrition, University of Gothenburg, Gothenburg, Sweden.
Jan
J
Lötvall
Department of Medicine, School of Medicine, Case Western Reserve University, Cleveland, Ohio, USA.
Lyndsay
L
Harris
Department of Nutrition, School of Medicine, Case Western Reserve University, Cleveland, Ohio, USA.
Cheryl L
CL
Thompson
Department of Pathology, School of Medicine, Case Western Reserve University, Cleveland, Ohio, USA.|The Case Comprehensive Cancer Center, Cleveland, Ohio, USA.|The National Center for Regenerative Medicine, Case Western Reserve University, Cleveland, Ohio, USA.|Department of Pharmacology, Feinberg School of Medicine, Northwestern University, Chicago, Illinois, USA.
Huiping
H
Liu
D014408:Biomarkers, Tumor / Q000097:blood / Q000235:genetics; D001798:Blood Proteins / Q000235:genetics*; D001943:Breast Neoplasms / Q000235:genetics; D051928:CD47 Antigen / Q000235:genetics; D045744:Cell Line, Tumor; D055354:Exosomes / Q000235:genetics*; D005260:Female; D020869:Gene Expression Profiling / Q000379:methods; D006801:Humans; D011506:Proteins / Q000235:genetics*; D059467:Transcriptome / Q000235:genetics*
D014408:Biomarkers, Tumor; D001798:Blood Proteins; D051928:CD47 Antigen; D011506:Proteins