The NF-kB regulates the SHP-1 expression in monocytes in congestive heart failure.
It has been shown that functional recovery of patients with acute congestive heart failure (ACHF) after treatment with conventional drugs (CD) is mediated by suppression of inflammation in peripheral blood mononuclear cells. Here, we analyzed gene expression profiles of monocytes from symptomatic ACHF patients (NYHA Class III-IV) before and after pharmacological treatment with CD. The treatment was associated with selective down-regulation of "TNFR signaling" and pro-inflammatory mediators CCL5, MIP-1α receptor, CD14, ITGAM, and significant up-regulation of "TNFR signaling" as evidenced by increase in anti-inflammatory factors including NF-kBIA, TNFAIP3 and SHP-1. In monocyte TNF-alpha-stimulated there is a down-regulation of the phosphatase SHP-1 which induces a significant activation of TAK-1/IKK/NF-kB signaling. These findings suggest that the therapeutic impact of CD treatment in symptomatic ACHF includes negative regulation of the NF-kB signaling in monocytes and the improvement of the SHP-1 activity.
Department of Psychological, Health and Territorial Sciences, University "G. D'Annunzio", Chieti, Italy.
Mirko
M
Pesce
Via dei Vestini, 31 66100 Chieti, Italy, s.franceschelli@unich.it.
Sara
S
Franceschelli
Department of Medicine and Science of Aging, University "G. D'Annunzio", Chieti, Italy.
Alessio
A
Ferrone
Department of Medicine and Science of Aging, University "G. D'Annunzio", Chieti, Italy.
Antonia
A
Patruno
Department of Psychological, Health and Territorial Sciences, University "G. D'Annunzio", Chieti, Italy.
Alfredo
A
Grilli
Department of Psychological, Health and Territorial Sciences, University "G. D'Annunzio", Chieti, Italy.
Maria Anna
MA
De Lutiis
IRCCS "S. Donato" Hospital, San Donato Milanese, Piazza Edmondo Malan, 20097 Milan, Italy.
Francesca R
FR
Pluchinotta
IRCCS "S. Donato" Hospital, San Donato Milanese, Piazza Edmondo Malan, 20097 Milan, Italy.
Sonia
S
Bergante
Department of Medicine and Science of Aging, University "G. D'Annunzio", Chieti, Italy, IRCCS "S. Donato" Hospital, San Donato Milanese, Piazza Edmondo Malan, 20097 Milan, Italy.
Guido
G
Tettamanti
Intensive Cardiology Care Unit, San Camillo de Lellis Hospital, San Severo (FG) 71016, Italy.
Graziano
G
Riccioni
Department of Medicine and Science of Aging, University "G. D'Annunzio", Chieti, Italy.
Mario
M
Felaco
Department of Medicine and Science of Aging, University "G. D'Annunzio", Chieti, Italy.
Lorenza
L
Speranza
D000368:Aged; D016022:Case-Control Studies; D005260:Female; D006333:Heart Failure / Q000097:blood* / Q000235:genetics; D006801:Humans; D051550:I-kappa B Kinase / Q000097:blood; D008214:Lymphocytes / Q000378:metabolism; D020930:MAP Kinase Kinase Kinases / Q000097:blood; D008297:Male; D008875:Middle Aged; D009000:Monocytes / Q000378:metabolism*; D016328:NF-kappa B / Q000097:blood*; D009504:Neutrophils / Q000378:metabolism; D053824:Protein Tyrosine Phosphatase, Non-Receptor Type 6 / Q000037:antagonists & inhibitors / Q000097:blood* / Q000235:genetics; D034741:RNA, Small Interfering / Q000235:genetics; D015398:Signal Transduction; D059467:Transcriptome; D014409:Tumor Necrosis Factor-alpha / Q000097:blood; D000099151:MAP Kinase Kinase Kinase 7
D051550:I-kappa B Kinase; D020930:MAP Kinase Kinase Kinases; D016328:NF-kappa B; D053824:Protein Tyrosine Phosphatase, Non-Receptor Type 6; D034741:RNA, Small Interfering; D014409:Tumor Necrosis Factor-alpha; D000099151:MAP Kinase Kinase Kinase 7; C509249:PTPN6 protein, human