Molecular Epidemiology of Multi-Drug ResistantAcinetobacter baumanniiIsolated in Shandong, China.
Acinetobacter baumanniiis an emerging nosocomial pathogen prevalent in hospitals worldwide. In order to understand the molecular epidemiology of multi-drug resistant (MDR)A. baumannii, we investigated the genotypes ofA. baumanniiisolated from 10 hospitals in Shandong, China, from August 2013 to December 2013, by pulsed field gel electrophoresis (PFGE) and multilocus sequence typing (MLST). Antimicrobial resistance genes were analyzed by PCR and DNA sequencing. By PFGE analysis, we discovered 11 PFGE types in these 10 hospitals. By MLST, we assigned these isolates to 12 sequence types (STs), 10 of which belong to the cloning complex CC92, including the prevalent ST369, ST208, ST195, and ST368. Two new STs, namely ST794 and ST809, were detected only in one hospital. All isolates of the MDRA. baumanniiwere resistant to carbapenem, except 2 isolates, which did not express theblaOXA-23carbapenemase gene, indicatingblaOXA-23is the major player for carbapenem resistance. We also discoveredarmAis likely to be responsible for amikacin resistance, and may play a role in gentamicin and tobramycin resistance.aac(3)-Iis another gene responsible for gentamicin and tobramycin resistance. In summary, we discovered that the majority of the isolates in Shandong, China, were the STs belonging to the CC92. Besides, two new STs were detected in one hospital. These new STs should be further investigated for prevention of outbreaks caused byA. baumannii.
The Department of Clinical Laboratory, Tai'an City Central Hospital Tai'an, China.
Meijie
M
Jiang
The Department of Hematology, Laiwu City People's Hospital Laiwu, China.
Lijuan
L
Liu
The Department of Clinical Laboratory, Zoucheng City People's Hospital Zoucheng, China.
Yunhua
Y
Ma
The Department of Clinical Laboratory, Tai'an City Central Hospital Tai'an, China.
Zhijun
Z
Zhang
The Department of Preventive Veterinary, College of Veterinary Medicine, Shandong Agricultural University Tai'an, China.
Ning
N
Li
Intensive Medicine, Tai'an City Central Hospital Tai'an, China.
Fusen
F
Zhang
The Department of Clinical Laboratory, Tai'an City Central Hospital Tai'an, China.
Shuping
S
Zhao
hospitals; molecular epidemiology; multi-drug resistant Acinetobacter baumannii; multilocus sequence typing; pulsed-field gel electrophoresis