Early Non-invasive Detection of Acute 1,2-Dichloroethane-induced Toxic Encephalopathy in Rats.
OBJECTIVE
To assess the acute effect of 1,2-dichloroethane (1,2-DCE) on rat brain using diffusion magnetic resonance imaging (dMRI).


METHODS
We performed dMRI on 30 male Sprague-Dawley rats, microstructural alterations were investigated by calculating the mean fractional anisotropy (FA) and apparent diffusion coefficient (ADC) changes in eight selected brain regions of interest. For the whole brain, clusters of 20+ voxels that differed significantly in FA and ADC between groups were marked. Hematoxylin-eosin staining was performed to confirm pathological changes.


RESULTS
Brain images showed lesions with brain edema in the white matter in both hemispheres in all groups exposed to 1,2-DCE. Diffusivity values were significantly different after 1,2-DCE inhalation (p<0.05).


CONCLUSIONS
Primarily cytotoxic edema occurred in acute 1,2-DCE-induced brain edema in rats. dMRI could be used for the early non-invasive detection of acute 1,2-DCE-induced toxic encephalopathy.
Department of Neurology, Xiangya Hospital, Central-South University, Changsha, P.R. China.
Xiaoliang
X
Zhou
Department of Neurology, Xiangya Hospital, Central-South University, Changsha, P.R. China.
Yuze
Y
Cao
Richard M.Lucas Center for Imaging, Department of Radiology, Stanford, CA, U.S.A.
Christoph
C
Leuze
Key Laboratory of Nuclear Analytical Techniques, Institute of High Energy Physics, Chinese Academy of Sciences, Beijing, P.R. China.
Binbin
B
Nie
Key Laboratory of Nuclear Analytical Techniques, Institute of High Energy Physics, Chinese Academy of Sciences, Beijing, P.R. China.
Baoci
B
Shan
Department of Neurology, Xiangya Hospital, Central-South University, Changsha, P.R. China.
Wenbin
W
Zhou
Richard M.Lucas Center for Imaging, Department of Radiology, Stanford, CA, U.S.A.
Peter
P
Cipriano
Department of Neurology, Xiangya Hospital, Central-South University, Changsha, P.R. China xiaobo62xy@126.com.
B O
BO
Xiao
D000818:Animals; D016880:Anisotropy; D001921:Brain / Q000000981:diagnostic imaging* / Q000187:drug effects / Q000473:pathology; D001929:Brain Edema / Q000139:chemically induced / Q000000981:diagnostic imaging; D038524:Diffusion Magnetic Resonance Imaging / Q000379:methods*; D005025:Ethylene Dichlorides / Q000633:toxicity*; D008297:Male; D020258:Neurotoxicity Syndromes / Q000000981:diagnostic imaging* / Q000209:etiology; D017207:Rats, Sprague-Dawley
D005025:Ethylene Dichlorides; C024565:ethylene dichloride
1,2-Dichloroethane exposure; brain edema; diffusion magnetic resonance imaging; toxic encephalopathy