Label-free attomolar detection of lactate based on radio frequency sputtered of nickel oxide thin film field effect transistor.
The radio frequency sputtered nickel oxide thin film nanostrtablucture deposited on glass substrate was used as a potential matrix for the realization of highly sensitive and selective field effect transistor-type lactate biosensor. Firstly, NiO-FET was tested for NADH detection showing a linear concentration range 1aM to 1nM and a low detection limit of 0.2aM. Then, NiO surface modified with chitosan and functionalized with glutaraldehyde and lactate dehydrogenase enzyme was immobilized on the aldehyde terminal. The biosensor is found to exhibit highly efficient sensing response characteristics with good linearity of 1aM to 1pM and low limit of detection of 0.5aM. The biosensor shows high stability without interferences from commonly interfering compounds in biological fluids, including uric acid, ascorbic acid, glucose and acetaminophen. Furthermore, the application of the proposed biosensor for analysis of lactate in artificial serum samples was evaluated with good satisfactory results. This protocol can be used to develop of disposable, low cost, and portable various types of dehydrogenase based biosensor devices using metal oxide nanomaterials.
Department of Chemistry, University of Kurdistan, 66177-15175 Sanandaj, Iran.
Samira
S
Mansouri Majd
Department of Chemistry, University of Kurdistan, 66177-15175 Sanandaj, Iran; Research Center for Nanotechnology, University of Kurdistan, 66177-15175 Sanandaj, Iran. Electronic address: absalimi@uok.ac.ir.
Abdollah
A
Salimi
Research Center for Nanotechnology, University of Kurdistan, 66177-15175 Sanandaj, Iran; Department of Physics, University of Kurdistan, 66177-15175 Sanandaj, Iran.
Bandar
B
Astinchap
D000818:Animals; D015374:Biosensing Techniques / Q000295:instrumentation*; D004800:Enzymes, Immobilized / Q000737:chemistry; D004867:Equipment Design; D006801:Humans; D007770:L-Lactate Dehydrogenase / Q000737:chemistry; D019344:Lactic Acid / Q000032:analysis / Q000097:blood*; D057230:Limit of Detection; D009532:Nickel / Q000737:chemistry*; D011817:Rabbits; D014173:Transistors, Electronic
D004800:Enzymes, Immobilized; D019344:Lactic Acid; D009532:Nickel; C028007:nickel monoxide; D007770:L-Lactate Dehydrogenase
Chitosan; Field effect transistor; Lactate biosensor; NiO thin film; Radio frequency sputtering