Effects of leukotriene D4 on adenoidal T cells in children with obstructive sleep apnea syndrome.
OBJECTIVE
This study aims to determine whether leukotriene D4 (LTD4) can promote T cell proliferation in adenoid tissuesviaactivation of CysLT1 receptors in children with OSAS.


METHODS
CD4+and CD8+T cell proliferation was assessed by flow cytometry in adenoid mononuclear cells (AdMCs) stimulated with LTD4 from children with OSAS. The activation of mitogen-activated protein kinase pathways and their effects on the proliferation of CD4+and CD8+T cells in AdMCs were observed by western blotting.


RESULTS
LTD4 increased the proliferation rates of both phytohemagglutinin (PHA)-stimulated CD4+T cells (15.5±8.4% in the PHA groupvs.24.8±6.3% in the PHA+LTD4 group;n=27;P<0.001) and CD8+T cells (17.2±5.9% in the PHA groupvs.23.5±5.2% in the PHA+LTD4 group;n=27;P<0.05) in AdMCs. LTD4 (10-4mmol) significantly increased the phosphorylation of extracellular signal-regulated kinase (ERK1/2) and p38, but not c-Jun N-terminal kinases (JNK). The ERK1/2 inhibitor PD98059 significantly inhibited the proliferation of CD4+and CD8+T cells in LTD4-stimulated AdMCs.


CONCLUSIONS
LTD4 regulates the proliferation of CD4+and CD8+T cells in PHA-stimulated AdMCsviaupregulation of the ERK1/2 pathway. This finding indicates that CysLT1 receptors play a regulatory role in the pathogenesis of OSAS in children.
Department of Otolaryngology, Children's Hospital of Chongqing Medical University, Ministry of Education Key Laboratory of Child Development and Disorders, China International Science and Technology Cooperation Base of Child Development and Critical Disorders, Chongqing Key Laboratory of Child Infection and Immunity Chongqing 400014, China.
Yan
Y
Shu
Department of Otolaryngology, Children's Hospital of Chongqing Medical University, Ministry of Education Key Laboratory of Child Development and Disorders, China International Science and Technology Cooperation Base of Child Development and Critical Disorders, Chongqing Key Laboratory of Child Infection and Immunity Chongqing 400014, China.
Da-Zhi
DZ
Yang
Department of Otolaryngology, Children's Hospital of Chongqing Medical University, Ministry of Education Key Laboratory of Child Development and Disorders, China International Science and Technology Cooperation Base of Child Development and Critical Disorders, Chongqing Key Laboratory of Child Infection and Immunity Chongqing 400014, China.
Jia
J
Liang
Department of Otolaryngology, Children's Hospital of Chongqing Medical University, Ministry of Education Key Laboratory of Child Development and Disorders, China International Science and Technology Cooperation Base of Child Development and Critical Disorders, Chongqing Key Laboratory of Child Infection and Immunity Chongqing 400014, China.
Feng
F
Zhang
Department of Otolaryngology, Children's Hospital of Chongqing Medical University, Ministry of Education Key Laboratory of Child Development and Disorders, China International Science and Technology Cooperation Base of Child Development and Critical Disorders, Chongqing Key Laboratory of Child Infection and Immunity Chongqing 400014, China.
Bing
B
Wang
Department of Otolaryngology, Children's Hospital of Chongqing Medical University, Ministry of Education Key Laboratory of Child Development and Disorders, China International Science and Technology Cooperation Base of Child Development and Critical Disorders, Chongqing Key Laboratory of Child Infection and Immunity Chongqing 400014, China.
Hong-Bing
HB
Yao
Department of Respiration Medicine, Children's Hospital of Chongqing Medical University, Ministry of Education Key Laboratory of Child Development and Disorders, China International Science and Technology Cooperation Base of Child Development and Critical Disorders, Chongqing Key Laboratory of Child Infection and Immunity Chongqing 400014, China.
En-Mei
EM
Liu
LTD4; MAPKs; OSAS; adenoid; proliferation