Type of chromosome abnormality affects embryo morphology dynamics.
To study the differences in the cleavage time between types of embryo chromosomal abnormalities and elaborate algorithm to exclude aneuploid embryos according to the likelihood to be euploid.



Retrospective cohort study.



University affiliated private center.



Preimplantational genetic screening patients (n = 112) including cases of advanced maternal age, repeated implantation failure, and recurrent miscarriage. A total of 485 embryos were analyzed.



None.



All biopsied embryos were cultured in an incubator with time-lapse technology, cleavage timing from insemination to day 3 and all kinetic parameters that have been described in previous studies by our group.



Logistic regression analysis were used to identify morphokinetic parameters and some were strongly associated with complex aneuploid embryos; t3 (odds ratio = 0.590, 95% confidence interval 0.359-0.971) and t5-t2 (odds ratio = 0.151, 95% confidence interval 0.082-0.278).



Embryo morphokinetics are affected by chromosome aneuploidy and further analysis of the chromosome content reveals higher differences when the complexity in the chromosome disorders is increased. The use of time-lapse monitoring, although not able to detect an abnormal embryo, may be potentially useful to discard those embryos with high risk of complex chromosomal abnormalities.
IVI Madrid, Madrid, Spain. Electronic address: Mamen.Nogales@ivi.es.
Maria
M
Del Carmen Nogales
IVI Madrid, Madrid, Spain.
Fernando
F
Bronet
IVI Madrid, Madrid, Spain.
Natalia
N
Basile
IVI Madrid, Madrid, Spain.
Eva María
EM
Martínez
IVI Madrid, Madrid, Spain.
Alberto
A
Liñán
IGENOMIX, Valencia, Spain.
Lorena
L
Rodrigo
Instituto Valenciano de Infertilidad, Universidad de Valencia, Valencia, Spain.
Marcos
M
Meseguer
D000328:Adult; D000782:Aneuploidy; D001706:Biopsy; D001755:Blastocyst / Q000473:pathology*; D002869:Chromosome Aberrations; D025063:Chromosome Disorders / Q000175:diagnosis* / Q000235:genetics / Q000473:pathology; D002877:Chromosomes, Human; D055028:Comparative Genomic Hybridization; D047108:Embryonic Development; D005260:Female; D005307:Fertilization in Vitro / Q000009:adverse effects*; D005820:Genetic Testing; D006801:Humans; D007700:Kinetics; D016015:Logistic Models; D018715:Microscopy, Video; D016017:Odds Ratio; D011237:Predictive Value of Tests; D011247:Pregnancy; D019836:Preimplantation Diagnosis / Q000379:methods; D012189:Retrospective Studies; D012307:Risk Factors; D059008:Time-Lapse Imaging / Q000379:methods
Embryo kinetics; array CGH; complex abnormalities; time lapse