Derivation of Patient Specific Pluripotent Stem Cells Using Clinically Discarded Cumulus Cells.
Induced pluripotent stem cells (iPSCs) are powerful tools for basic and translational research, as well as regenerative medicine. In routine human in vitro fertilization (IVF) practices, cumulus cells (CCs) are discarded, representing a potential source of biological materials for regenerative medicine. In this study, we derived patient-specific iPSCs using CCs from human infertility clinics for the first time. The human cumulus cell derived iPSCs (hc-iPSCs) were characterized for growth, karyotype, expression of pluripotency genes, and were subjected to embryoid bodies (EBs) and teratoma assays to evaluate their differentiation capacity. Hc-iPSCs display typical iPSC characteristics, and are capable of differentiating into all germ layers in vitro and in vivo. We further show that putative primordial germ cell like cells (PGCLCs) can be derived using hc-iPSCs. Our data demonstrate the feasibility of deriving patient-specific pluripotent stem cells using CCs.
Institute of Biotechnology, National Taiwan University, Taipei, Taiwan.
Wei-Fang
WF
Chang
Department of Obstetrics and Gynecology, Mackay Memorial Hospital, Taipei, Taiwan.|Department of Obstetrics and Gynecology, Mackay Medical College, New Taipei City, Taiwan.|Department of Obstetrics and Gynecology, Mackay Junior College of Medicine, Nursing, and Management, Taipei, Taiwan.
Yuh-Ming
YM
Hwu
Center for Advanced Models for Translational Sciences and Therapeutics, University of Michigan Medical Center, Ann Arbor, Michigan, United States of America.
Jie
J
Xu
Department of Obstetrics and Gynecology, Mackay Memorial Hospital, Taipei, Taiwan.|Department of Obstetrics and Gynecology, Mackay Medical College, New Taipei City, Taiwan.
Chen-Ju
CJ
Lin
Institute of Biotechnology, National Taiwan University, Taipei, Taiwan.
Sheng-Wen
SW
Wang
Yupintang Traditional Chinese Medicine Foundation, Kaohsiung, Taiwan.
An-Sheng
AS
Cheng
Genomics Research Center, Academia Sinica, Taipei, Taiwan.
Jean
J
Lu
Department of Obstetrics and Gynecology, Mackay Memorial Hospital, Taipei, Taiwan.
Chung-Hao
CH
Lu
Institute of Biotechnology, National Taiwan University, Taipei, Taiwan.|Center for Biotechnology, National Taiwan University, Taipei, Taiwan.|Animal Resource Center, National Taiwan University, Taipei, Taiwan.|Agricultural Biotechnology Research Center, Academia Sinica, Taipei, Taiwan.
Li-Ying
LY
Sung
D000328:Adult; D002454:Cell Differentiation; D041321:Chromosomes, Human, X / Q000235:genetics; D054885:Cumulus Cells / Q000166:cytology*; D005260:Female; D005786:Gene Expression Regulation; D005855:Germ Layers / Q000166:cytology; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000166:cytology* / Q000378:metabolism