Effect of 20-Hydroxyecdysone on Proteolytic Regulation in Skeletal Muscle Atrophy.
OBJECTIVE
20-Hydroxyecdystone (20E) is an ecdysteroid hormone which controls molting and reproduction in arthropods. 20E also produces a variety of effects in vertebrates, including enhancing protein synthesis and skeletal muscle regeneration. The effect of 20E on disuse muscle atrophy has not been reported to date. This study examined the proteolytic regulation of 20E in tenotomized rat slow soleus and fast plantaris muscles.


METHODS
Male Wistar rats were randomly divided into three groups: sedentary control (CON), tenotomy without 20E treatment (TEN), and tenotomy with treatment of 5 mg/kg BW of 20E (TEN+20E). The TEN+20E group was administered 20E via subcutaneous injection to the right thigh for 7 days after tenotomy.


RESULTS
20E treatment tended to attenuate disuse muscle atrophy and reduced ubiquitination only in soleus muscle.


CONCLUSIONS
20E treatment alleviates skeletal muscle atrophy partially mediated by ubiquitinate pathway, dependent on the muscle phenotype.
Department of Biopharmacy, Faculty of Pharmacy, Srinakharinwirot University, Nakhon Nayok, Thailand muthitah@hotmail.com muthita@swu.ac.th.|Department of Physiology, Faculty of Science, Mahidol University, Bangkok, Thailand.
Muthita
M
Hirunsai
Department of Physiology, Faculty of Science, Mahidol University, Bangkok, Thailand.|Faculty of Sports Science, Chulalongkorn University, Pathumwan, Bangkok, Thailand.
Tossaporn
T
Yimlamai
Department of Chemistry, Faculty of Science, Ramkhamhaeng University, Bangkapi, Bangkok, Thailand.
Apichart
A
Suksamrarn
D000818:Animals; D015153:Blotting, Western; D004441:Ecdysterone / Q000008:administration & dosage / Q000494:pharmacology*; D007279:Injections, Subcutaneous; D008297:Male; D018482:Muscle, Skeletal / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D009133:Muscular Atrophy / Q000378:metabolism / Q000517:prevention & control*; D059748:Proteolysis / Q000187:drug effects*; D011897:Random Allocation; D017208:Rats, Wistar; D015398:Signal Transduction / Q000187:drug effects; D058971:Tenotomy; D057149:Ubiquitinated Proteins / Q000378:metabolism; D054875:Ubiquitination / Q000187:drug effects
D057149:Ubiquitinated Proteins; D004441:Ecdysterone
Ecdysteroid; MURF1; muscle-type specific; tenotomy; ubiquitin