Cloning and expression ofSgCYP450-4fromSiraitia grosvenorii.
CYP450 plays an essential role in the development and growth of the fruits ofSiraitia grosvenorii. However, little is known about theSgCYP450-4gene inS. grosvenorii. Here, based on transcriptome data, a full-length cDNA sequence ofSgCYP450-4was cloned by reverse transcriptase-polymerase chain reaction (RT-PCR) and rapid-amplification of cDNA ends (RACE) strategies.SgCYP450-4is 1677 bp in length (GenBank accession No. AEM42985.1) and contains a complete open reading frame (ORF) of 1422 bp. The deduced protein was composed of 473 amino acids, the molecular weight is 54.01 kDa, the theoretical isoelectric point (PI) is 8.8, and the protein was predicted to possess cytochrome P450 domains.SgCYP450-4gene was highly expressed in root, diploid fruit and fruit treated with hormone and pollination. At 10 days after treatment with pollination and hormones, the expression of SgCYP450-4had the highest level and then decreased over time, which was consistent with the development of fruits ofS. Grosvenorii. Hormonal treatment could significantly induce the expression ofSgCYP450-4. These results provide a reference for regulation of fruit development and the use of parthenocarpy to generate seedless fruit, and provide a scientific basis for the production of growth regulator application agents.
Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Peking 100193, China.
Dongping
D
Tu
Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Peking 100193, China; Yunnan Branch Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Jinghong 530023, China.
Xiaojun
X
Ma
Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Peking 100193, China.
Huan
H
Zhao
Guangxi Botanical Garden of Medicinal Plants, Nanning 530023, China.
Changming
C
Mo
Horticulture & Landscape College, Hunan Agriculture University, Changsha 410128, China.
Qi
Q
Tang
Guangxi University of Chinese Medicine, Nanning 530001, China.
Liuping
L
Wang
Guangxi University of Chinese Medicine, Nanning 530001, China.
Jie
J
Huang
Guangxi Botanical Garden of Medicinal Plants, Nanning 530023, China.
Limei
L
Pan
Auxin; CYP87A3; Expression pattern; Race clone; SgCYP450-4; Siraitia grosvenorii