Identification of Lung Inflammation-Related Elevation of Acylamino Acid Releasing Enzyme (APEH) Activity Using an Enzymomics Approach.
Disease-associated alterations of enzymatic functions are potentially useful as disease biomarkers, and here we show that an enzymomics (omics of active enzymes) approach, in which enzymatic activities are screened with panels of substrates, can be an effective way to identify such alterations. In the present study, we used a panel of fluorogenic substrates to search for altered enzyme activities in bronchoalveolar lavage fluid (BALF) from a mouse model of lung inflammation. We found that acylamino acid releasing enzyme (APEH) activity was highly elevated, apparently reflecting the increased population of immune cells in the inflamed lung.
Graduate School of Pharmaceutical Sciences, The University of Tokyo.
Toru
T
Komatsu
Kentaro
K
Yoshioka
Kenjiro
K
Hanaoka
Takuya
T
Terai
Tasuku
T
Ueno
Tetsuo
T
Nagano
Yasuteru
Y
Urano
D000818:Animals; D001992:Bronchoalveolar Lavage Fluid / Q000276:immunology; D004195:Disease Models, Animal; D004789:Enzyme Activation; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000737:chemistry; D005470:Fluorometry; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D015394:Molecular Structure; D010447:Peptide Hydrolases / Q000276:immunology / Q000378:metabolism*; D011014:Pneumonia / Q000201:enzymology* / Q000276:immunology / Q000378:metabolism
D005456:Fluorescent Dyes; D010447:Peptide Hydrolases; C038220:acylaminoacyl-peptidase