In Vitro Proinflammatory Polarization of Macrophages Isolated from Hepatocarcinogenic Stage in Humans and Rats.
Macrophages have multiple functions in both inhibiting and promoting hepatocarcinogenesis, which are dependent on their phenotypes. Thus, we were interested in clarifying the 'training' proinflammatory effects exerted by barley β-glucan (BBG) on monocyte-derived macrophages from patients with hepatocellular carcinoma (HCC-Mfs). After isolation and differentiation HCC-Mfs were treated with different concentrations of BBG and functional assays were then conducted after 24 h, and 3 and 5 days of incubation. The release of reactive oxygen species, arginase concentration and cell morphology were analyzed. Under the influence of BBG neoplastic cells slightly elongated and dendric-like filopodia were observed. In HCC-Mfs, the significant generation of NO and O2-was seen on days 3 and 5 of culture, concomitantly with significant depletion (p<0.05) of arginase activity. In summary, we showed that HCC-Mfs, 'trained' in a BBG microenvironment keep a highly dynamic plasticity, together with their proinflammatory polarization, expressed by reactive oxygen species and reactive nitrogen intermediates (RNI) augmentation.
Department of Pathophysiology, Faculty of Veterinary Medicine, University of Life Sciences in Lublin, Lublin, Poland marta.wojcik@up.lublin.pl.
Marta
M
Wójcik
Department of Pathophysiology, Faculty of Veterinary Medicine, University of Life Sciences in Lublin, Lublin, Poland.
Joanna
J
Wessely-Szponder
Department of Gastroenterology with Endoscopic Unit, II Faculty of Medicine with English Language Division, Medical University in Lublin, Lublin, Poland.
Halina
H
Cichoż-Lach
Department of Gastroenterology with Endoscopic Unit, II Faculty of Medicine with English Language Division, Medical University in Lublin, Lublin, Poland.
Krzysztof
K
Celiński
Department of Pathophysiology, Faculty of Veterinary Medicine, University of Life Sciences in Lublin, Lublin, Poland.
Ryszard
R
Bobowiec
D000818:Animals; D006528:Carcinoma, Hepatocellular / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D002478:Cells, Cultured; D060833:Cellular Microenvironment / Q000187:drug effects / Q000276:immunology*; D005260:Female; D001467:Hordeum / Q000737:chemistry; D006801:Humans; D007249:Inflammation / Q000276:immunology; D008113:Liver Neoplasms / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D008262:Macrophage Activation / Q000187:drug effects / Q000276:immunology*; D008264:Macrophages / Q000187:drug effects / Q000276:immunology* / Q000378:metabolism; D009569:Nitric Oxide / Q000276:immunology / Q000378:metabolism; D011554:Pseudopodia / Q000187:drug effects / Q000276:immunology; D017208:Rats, Wistar; D017382:Reactive Oxygen Species / Q000276:immunology / Q000378:metabolism; D013045:Species Specificity; D047071:beta-Glucans / Q000276:immunology* / Q000494:pharmacology
D017382:Reactive Oxygen Species; D047071:beta-Glucans; D009569:Nitric Oxide
Macrophages; hepatocellular carcinoma; trained immunity; β-glucan