NRAMP1 Polymorphisms like Susceptibility Marker in Mexican Focus of Cutaneous Leishmaniasis.
Cutaneous leishmaniasis (CL) is endemic in Campeche state, Mexico. Host and parasite factors are involved in the establishment and development of CL. Host factors include immune response and genetic background. NRAMP1 (Natural Resistance Associated Macrophage Protein 1) is important in innate immunity. Polymorphisms inNRAMP1have been associated with susceptibility or resistance to infectious and autoimmune diseases. To study the association ofNRAMP1mutations with CL in patients from Calakmul, Campeche, samples from 115 CL patients and 69 samples of healthy people from the same area were evaluated. Five regions inNRAMP1were amplified and digested, looking for mutations in the promoter region (-524G/C), exon 3 (274C/T), exon 8 (823 C7T), and exon 15 (G/A) and deletion of 4 bp in the 3'UTR region. We found a statistical association between polymorphisms in 3'UTR region and exon 8 and CL [χ2= 13.26;p< 0.05; OR = 17.00; IC of 95% (2.24-128.99)]. Some patients who needed more than 40 doses of Glucantime® to heal injuries presented mutations in exons 3, 8, and 15. Multiple or ear lesions were not associated withNRAMP1polymorphism.
Departamento de Inmunología, Escuela Nacional de Ciencias Biológicas, IPN, Carpio y Plan de Ayala, 11340 Mexico City, Mexico.
Mirsha Pamela
MP
Hernández-Rivera
Departamento de Infectología e Inmunología, Instituto Nacional de Perinatología, Montes Urales 800, 11000 Mexico City, Mexico.
Alicia
A
Ramírez-Ramírez
H. Ayuntamiento de Calakmul, Halaltun, 24640 Xpujil, CAM, Mexico.
Adelaido
A
Chiñas-Pérez
Departamento de Inmunología, Escuela Nacional de Ciencias Biológicas, IPN, Carpio y Plan de Ayala, 11340 Mexico City, Mexico.
Amalia
0000-0001-9942-2737
A
Monroy-Ostria
Departamento de Inmunología, Escuela Nacional de Ciencias Biológicas, IPN, Carpio y Plan de Ayala, 11340 Mexico City, Mexico.
Mario Eugenio
ME
Cancino-Díaz
Departamento de Inmunología, Escuela Nacional de Ciencias Biológicas, IPN, Carpio y Plan de Ayala, 11340 Mexico City, Mexico.
Omar
0000-0001-7455-0070
O
Hernández-Montes
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D027682:Cation Transport Proteins / Q000235:genetics*; D002648:Child; D005260:Female; D005819:Genetic Markers / Q000235:genetics; D020022:Genetic Predisposition to Disease / Q000453:epidemiology* / Q000235:genetics*; D006801:Humans; D016773:Leishmaniasis, Cutaneous / Q000175:diagnosis / Q000453:epidemiology* / Q000235:genetics*; D008297:Male; D008800:Mexico / Q000453:epidemiology; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D015995:Prevalence; D015203:Reproducibility of Results; D012307:Risk Factors; D012680:Sensitivity and Specificity; D000099173:Natural Resistance-associated Macrophage Protein 1
D027682:Cation Transport Proteins; D005819:Genetic Markers; D000099173:Natural Resistance-associated Macrophage Protein 1