Thermo-Responsive Complexes of c-Myc Antisense Oligonucleotide with Block Copolymer of Poly(OEGMA) and Quaternized Poly(4-Vinylpyridine).
Solution behavior of thermo-responsive polymers and their complexes with biological macromolecules may be affected by environmental conditions, such as the concentration of macromolecular components, pH, ion concentration, etc. Therefore, a thermo-responsive polymer and its complexes should be characterized in detail to observe their responses against possible environments under physiological conditions before biological applications. To briefly indicate this important issue, thermo-responsive block copolymer of quaternized poly(4-vinylpyridine) and poly(oligoethyleneglycol methyl ether methacrylate) as a potential nonviral vector has been synthesized. Polyelectrolyte complexes of this copolymer with the antisense oligonucleotide of c-Myc oncogene are also thermo-responsive but, have lower LCST (lower critical solution temperature) values compared to individual copolymer. LCST values of complexes decrease with molar ratio of macromolecular components and presence of salt. Dilution of solutions also affects solution behavior of complexes and causes a significant decrease in size and an increase in LCST, which indicates possible effects of severe dilutions in the blood stream.
Department of Bioengineering, Yildiz Technical University, Istanbul, 34210, Turkey.
Murat
M
Topuzogullari
Department of Bioengineering, Yildiz Technical University, Istanbul, 34210, Turkey.
Yeliz Basaran
YB
Elalmis
Bioengineering Department, Abdullah Gul University, Kayseri, 38170, Turkey.
Sevil Dincer
SD
Isoglu
D005453:Fluorescence; D057446:Hydrodynamics; D008689:Methacrylates / Q000737:chemistry*; D018625:Microscopy, Atomic Force; D016376:Oligonucleotides, Antisense / Q000737:chemistry*; D000071228:Polyelectrolytes / Q000737:chemistry; D011092:Polyethylene Glycols / Q000737:chemistry*; D011109:Polymethacrylic Acids; D011145:Polyvinyls / Q000737:chemistry*; D016271:Proto-Oncogene Proteins c-myc / Q000378:metabolism*; D066244:Proton Magnetic Resonance Spectroscopy; D055672:Static Electricity; D013696:Temperature
D008689:Methacrylates; D016376:Oligonucleotides, Antisense; D000071228:Polyelectrolytes; D011109:Polymethacrylic Acids; D011145:Polyvinyls; D016271:Proto-Oncogene Proteins c-myc; C528061:poly(poly(ethylene glycol)methacrylate); C019535:poly(4-vinylpyridine); D011092:Polyethylene Glycols
nonviral vector; polyelectrolyte complex; temperature-responsive polymer