Heat-Killed Lactobacillus salivarius and Lactobacillus johnsonii Reduce Liver Injury Induced by Alcohol In Vitro and In Vivo.
The aim of the present study was to determine whetherLactobacillus salivarius(LS) andLactobacillus johnsonii(LJ) prevent alcoholic liver damage in HepG2 cells and rat models of acute alcohol exposure. In this study, heat-killed LS and LJ were screened from 50Lactobacillusstrains induced by 100 mM alcohol in HepG2 cells. The severity of alcoholic liver injury was determined by measuring the levels of aspartate transaminase (AST), alanine transaminase (ALT), gamma-glutamyl transferase (γ-GT), lipid peroxidation, triglyceride (TG) and total cholesterol. Our results indicated that heat-killed LS and LJ reduced AST, ALT, γ-GT and malondialdehyde (MDA) levels and outperformed other bacterial strains in cell line studies. We further evaluated these findings by administering these strains to rats. Only LS was able to reduce serum AST levels, which it did by 26.2%. In addition LS significantly inhibited serum TG levels by 39.2%. However, both strains were unable to inhibit ALT levels. In summary, we demonstrated that heat-killed LS and LJ possess hepatoprotective properties induced by alcohol both in vitro and in vivo.
Department of Nutrition, Master Program of Biomedical Nutrition, Hungkuang University, 1018 Sec. 6 Taiwan Boulevard, Taichung 43302, Taiwan. chchuang@hk.edu.tw.
Cheng-Hung
CH
Chuang
Department of Food Science and Technology, Hungkuang University, Taichung 43302, Taiwan. tsaicc@sunrise.hk.edu.tw.
Cheng-Chih
CC
Tsai
Department of Beauty Science, National Taichung University of Science and Technology, No. 193, Sec. 1, San-Min Rd., Taichung 40343, Taiwan. eslin7620@hotmail.com.
En-Shyh
ES
Lin
Department of Health and Nutrition Biotechnology, Asia University, 500 Lioufeng Rd., Wufeng, Taichung 41354, Taiwan. cshuang@asia.edu.tw.|Department of Medical Research, China Medical University Hospital, China Medical University, Taichung 40402, Taiwan. cshuang@asia.edu.tw.
Chin-Shiu
CS
Huang
Department of Nutrition, Master Program of Biomedical Nutrition, Hungkuang University, 1018 Sec. 6 Taiwan Boulevard, Taichung 43302, Taiwan. lin10554@gmail.com.
Yun-Yu
YY
Lin
New Bellus Enterprises Co., Ltd. No. 48, Industrial Rd., Erh Chen Vil., Kuan Tien Dist., Tainan 72042, Taiwan. alicelan.nb@gmail.com.
Chuan-Ching
CC
Lan
New Bellus Enterprises Co., Ltd. No. 48, Industrial Rd., Erh Chen Vil., Kuan Tien Dist., Tainan 72042, Taiwan. john@newbellus.com.tw.
Chun-Chih
CC
Huang
D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D001219:Aspartate Aminotransferases / Q000097:blood; D002784:Cholesterol / Q000097:blood; D056945:Hep G2 Cells; D006519:Hepatitis, Alcoholic / Q000097:blood / Q000188:drug therapy*; D006801:Humans; D000069999:Lactobacillus johnsonii; D000069996:Ligilactobacillus salivarius; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D014280:Triglycerides / Q000097:blood; D005723:gamma-Glutamyltransferase / Q000097:blood
D014280:Triglycerides; D002784:Cholesterol; D005723:gamma-Glutamyltransferase; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase
Lactobacillus johnsonii; Lactobacillus salivariu; alcoholic liver disease; heat-killed Lactobacillus; malondialdehyde; γ-glutamyl transferase