Image analysis for a microfluidic paper-based analytical device using the CIE L*a*b* color system.
The combination of a microfluidic paper-based analytical device (μPAD) and digital image analysis is widely used for quantitative analysis with μPADs because of its easy and simple operation. Herein, we have demonstrated a quantitative analysis based on multiple color changes on a μPAD. The CIE L*a*b* color system was employed to analyse the digital images obtained with the μPAD. We made pH measurements using a universal pH-indicator showing multiple color changes for various pH values of aqueous test solutions. The detectable pH range of this method was wider than the typical grayscale-based image analysis, and we succeeded in the measurements for a wide pH range of 2-9.
Graduate School of Chemical Sciences and Engineering, Hokkaido University, Kita 13 Nishi8, Kita-ku, Sapporo 060-8628, Japan.
Takeshi
T
Komatsu
Graduate School of Chemical Sciences and Engineering, Hokkaido University, Kita 13 Nishi8, Kita-ku, Sapporo 060-8628, Japan.
Saeed
S
Mohammadi
Graduate School of Chemical Sciences and Engineering, Hokkaido University, Kita 13 Nishi8, Kita-ku, Sapporo 060-8628, Japan.
Lori Shayne Alamo
LS
Busa
Division of Applied Chemistry, Faculty of Engineering, Hokkaido University, Kita 13 Nishi 8, Kita-ku, Sapporo 060-8628, Japan. ishida-a@eng.hokudai.ac.jp.
Masatoshi
M
Maeki
Division of Applied Chemistry, Faculty of Engineering, Hokkaido University, Kita 13 Nishi 8, Kita-ku, Sapporo 060-8628, Japan. ishida-a@eng.hokudai.ac.jp.
Akihiko
A
Ishida
Division of Applied Chemistry, Faculty of Engineering, Hokkaido University, Kita 13 Nishi 8, Kita-ku, Sapporo 060-8628, Japan. ishida-a@eng.hokudai.ac.jp.
Hirofumi
H
Tani
Division of Applied Chemistry, Faculty of Engineering, Hokkaido University, Kita 13 Nishi 8, Kita-ku, Sapporo 060-8628, Japan. ishida-a@eng.hokudai.ac.jp and ImPACT Research Centre for Advanced Nanobiodevices, Nagoya University, Furo-cho Chikusa-ku, Nagoya 464-8603, Japan and Innovative Research Centre for Preventive Medical Engineering, Nagoya University, Furo-cho Chikusa-ku, Nagoya 464-8603, Japan and Institute of Innovation for Future Society, Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8603, Japan. tokeshi@eng.hokudai.ac.jp.
Manabu
M
Tokeshi