Olfactomedin-like 2 A and B (OLFML2A and OLFML2B) expression profile in primates (human and baboon).
BACKGROUND
The olfactomedin-like domain (OLFML) is present in at least four families of proteins, including OLFML2A and OLFML2B, which are expressed in adult rat retina cells. However, no expression of their orthologous has ever been reported in human and baboon.


OBJECTIVE
The aim of this study was to investigate the expression of OLFML2A and OLFML2B in ocular tissues of baboons (Papio hamadryas) and humans, as a key to elucidate OLFML function in eye physiology.


METHODS
OLFML2A and OLFML2B cDNA detection in ocular tissues of these species was performed by RT-PCR. The amplicons were cloned and sequenced, phylogenetically analyzed and their proteins products were confirmed by immunofluorescence assays.


RESULTS
OLFML2A and OLFML2B transcripts were found in human cornea, lens and retina and in baboon cornea, lens, iris and retina. The baboon OLFML2A and OLFML2B ORF sequences have 96% similarity with their human's orthologous. OLFML2A and OLFML2B evolution fits the hypothesis of purifying selection. Phylogenetic analysis shows clear orthology in OLFML2A genes, while OLFML2B orthology is not clear.


CONCLUSIONS
Expression of OLFML2A and OLFML2B in human and baboon ocular tissues, including their high similarity, make the baboon a powerful model to deduce the physiological and/or metabolic function of these proteins in the eye.
Servicio de Oncología, Universidad Autónoma de Nuevo León, Hospital Universitario "Dr. José Eleuterio González", Monterrey, Nuevo León, Mexico.
Diana Cristina
DC
Pérez-Ibave
Facultad de Medicina, Universidad Autónoma de Guadalajara, Zapopan, Jalisco, Mexico.
Rafael
R
González-Alvarez
Unidad Académica de Medicina Humana y Ciencias de la Salud, Universidad Autónoma de Zacatecas, Carretera Zacatecas-Guadalajara Km.6. Ejido La Escondida, Zacatecas, Mexico.
Margarita
M
de La Luz Martinez-Fierro
Departamento de Ecología, Facultad de Ciencias Biológicas, Universidad Autónoma de Nuevo León, 66451, San Nicolás de los Garza, Nuevo León, Mexico.
Gabriel
G
Ruiz-Ayma
Departamento de Neurobiología Celular y Molecular, Instituto de Neurobiología, Universidad Nacional Autónoma de México, Querétaro, Querétaro, Mexico.
Maricela
M
Luna-Muñoz
Departamento de Genética, Universidad Autónoma de Nuevo León, Hospital Universitario "Dr. José Eleuterio González", 64460, Monterrey, Nuevo León, Mexico.
Laura Elia
LE
Martínez-De-Villarreal
Departamento de Bioquímica y Medicina Molecular, Facultad de Medicina, Universidad Autónoma de Nuevo León, Monterrey, Nuevo León, Mexico.
María
M
De Lourdes Garza-Rodríguez
Departamento de Biología Celular y Genética, Facultad de Ciencias Biológicas, Universidad Autónoma de Nuevo León, Monterrey, Nuevo León, Mexico.
Diana
D
Reséndez-Pérez
Departamento de Oftalmología, Universidad Autónoma de Nuevo León Hospital Universitario "Dr. José Eleuterio González", Monterrey, Nuevo León, Mexico.
Jibran
J
Mohamed-Noriega
Servicio de Anatomía Patológica y Citopatología, Universidad Autónoma de Nuevo León, Hospital Universitario "Dr. José Eleuterio González", Monterrey, Nuevo León, Mexico.
Raquel
R
Garza-Guajardo
Departamento de Bioquímica y Medicina Molecular, Instituto de Oftalmología. Fundación de Asistencia Privada Conde de Valenciana IAP, Mexico, Mexico.
Víctor Manuel
VM
Bautista-De-Lucío
Departamento de Oftalmología, Universidad Autónoma de Nuevo León Hospital Universitario "Dr. José Eleuterio González", Monterrey, Nuevo León, Mexico.
Karim
K
Mohamed-Noriega
Servicio de Anatomía Patológica y Citopatología, Universidad Autónoma de Nuevo León, Hospital Universitario "Dr. José Eleuterio González", Monterrey, Nuevo León, Mexico.
Oralia
O
Barboza-Quintana
Departamento de Neurobiología Celular y Molecular, Instituto de Neurobiología, Universidad Nacional Autónoma de México, Querétaro, Querétaro, Mexico.
Carlos
C
Arámburo-De-La-Hoz
Departamento de Bioquímica y Medicina Molecular, Facultad de Medicina, Universidad Autónoma de Nuevo León, Monterrey, Nuevo León, Mexico.
Hugo Alberto
HA
Barrera-Saldaña
Departamento de Genética, Universidad Autónoma de Nuevo León, Hospital Universitario "Dr. José Eleuterio González", 64460, Monterrey, Nuevo León, Mexico. iramrodriguez@gmail.com.
Irám Pablo
IP
Rodríguez-Sánchez
D000818:Animals; D058893:DNA Barcoding, Taxonomic; D019143:Evolution, Molecular; D016326:Extracellular Matrix Proteins / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D005123:Eye / Q000737:chemistry / Q000378:metabolism*; D005455:Fluorescent Antibody Technique / Q000379:methods; D006023:Glycoproteins / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D008565:Membrane Proteins / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D009799:Ocular Physiological Phenomena; D010215:Papio; D012016:Reference Values; D020133:Reverse Transcriptase Polymerase Chain Reaction; D048348:Reverse Transcription; D020539:Sequence Analysis, Protein
D016326:Extracellular Matrix Proteins; D006023:Glycoproteins; D008565:Membrane Proteins; C581693:Olfml2a protein, human; C081451:olfactomedin
Baboon; Eye; OLFML2; Old world monkey; Olfactomedin