miR-1 association with cell proliferation inhibition and apoptosis in vestibular schwannoma by targeting VEGFA.
A growing body of research has demonstrated the tumor suppressive function of microRNA (miR)-1 in many cancers. Our study aimed to investigate its role in vestibular schwannoma (VS). We examined miR-1 expression in 95 VS specimens and 79 normal vestibular nerves using quantitative real-time polymerase chain reaction. Moreover, miR-1 mimics, miR-1 inhibitors, and negative control oligonucleotides were transfected into HEI-193 human VS cells to investigate the functional significance of miR-1 expression in this condition at a cellular level. Finally, the role of vascular endothelial growth factor A (VEGFA) in miR-1-mediated HEI-193 cell growth was confirmed. miR-1 levels were significantly reduced in VS specimens compared with normal vestibular nerve tissues (P < 0.001). In addition, low levels of miR-1 were associated with larger tumor volumes. In functional assays, miR-1 suppressed HEI-193 cell proliferation and colony formation, and enhanced apoptosis. VEGFA was verified as a target gene of miR-1, and VEGFA overexpression partially negated the effects of miR-1 on HEI-193 cells. These findings suggest that miR-1 suppresses VS growth by targeting VEGFA, and should be considered as a potential therapeutic target for treatment of this condition.
Department of Neurology, Xinxiang Central Hospital, Xinxiang, Henan, China.
S L
SL
Li
Department of Neurology, Xinxiang Central Hospital, Xinxiang, Henan, China drhnnywst@163.com.
X H
XH
Ma
Department of Neurology, Xinxiang Central Hospital, Xinxiang, Henan, China.
J F
JF
Ji
Department of Neurology, Xinxiang Central Hospital, Xinxiang, Henan, China.
H
H
Li
Department of Neurology, Xinxiang Central Hospital, Xinxiang, Henan, China.
W
W
Liu
Department of Neurology, Xinxiang Central Hospital, Xinxiang, Henan, China.
F Z
FZ
Lu
Department of Neurology, Xinxiang Central Hospital, Xinxiang, Henan, China.
S T
ST
Wu
Department of Neurology, Xinxiang Central Hospital, Xinxiang, Henan, China.
Y
Y
Zhang
D017209:Apoptosis / Q000235:genetics*; D001483:Base Sequence; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D015972:Gene Expression Regulation, Neoplastic; D056726:Genetic Association Studies; D006801:Humans; D035683:MicroRNAs; D009464:Neuroma, Acoustic / Q000235:genetics* / Q000473:pathology*; D014410:Tumor Stem Cell Assay; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism*
C544547:MIRN1 microRNA, human; D035683:MicroRNAs; C467484:VEGFA protein, human; D042461:Vascular Endothelial Growth Factor A