The SURMetaGIT study: Design and rationale for a prospective pan-omics examination of the gastrointestinal response to Roux-en-Y gastric bypass surgery.
Objective To describe the protocol of the SURgically induced Metabolic effects on the Human GastroIntestinal Tract (SURMetaGIT) study, a clinical pan-omics study exploring the gastrointestinal tract as a central organ driving remission of type 2 diabetes mellitus (T2DM) after Roux-en-Y gastric bypass (RYGB). The main points considered in the study's design and challenges faced in its application are detailed. Methods This observational, longitudinal, prospective study involved collection of gastrointestinal biopsy specimens, faeces, urine, and blood from 25 obese women with T2DM who were candidates for RYGB (20 patients for omics assessment and 5 for omics validation). These collections were performed preoperatively and 3 and 24 months postoperatively. Gastrointestinal transcriptomics; faecal metagenomics and metabolomics; plasma proteomics, lipidomics, and metabolomics; and biochemical, nutritional, and metabolic data were assessed to identify their short- and long-term correlations with T2DM remission. Results Data were collected from 20 patients before and 3 months after RYGB. These patients have nearly completed the 2-year follow-up assessments. The five additional patients are currently being selected for omics data validation. Conclusion The multi-integrated pan-omics approach of the SURMetaGIT study enables integrated analysis of data that will contribute to the understanding of molecular mechanisms involved in T2DM remission after RYGB.
1 Department of Gastroenterology, Digestive Surgery Discipline, School of Medicine, FMUSP - University of São Paulo, São Paulo, Brazil.
Priscila
P
Sala
1 Department of Gastroenterology, Digestive Surgery Discipline, School of Medicine, FMUSP - University of São Paulo, São Paulo, Brazil.
Giliane
G
Belarmino
1 Department of Gastroenterology, Digestive Surgery Discipline, School of Medicine, FMUSP - University of São Paulo, São Paulo, Brazil.
Natasha Mendonça
NM
Machado
1 Department of Gastroenterology, Digestive Surgery Discipline, School of Medicine, FMUSP - University of São Paulo, São Paulo, Brazil.
Camila Siqueira
CS
Cardinelli
1 Department of Gastroenterology, Digestive Surgery Discipline, School of Medicine, FMUSP - University of São Paulo, São Paulo, Brazil.
Karina
K
Al Assal
1 Department of Gastroenterology, Digestive Surgery Discipline, School of Medicine, FMUSP - University of São Paulo, São Paulo, Brazil.
Mariane Marques
MM
Silva
1 Department of Gastroenterology, Digestive Surgery Discipline, School of Medicine, FMUSP - University of São Paulo, São Paulo, Brazil.
Danielle Cristina
DC
Fonseca
1 Department of Gastroenterology, Digestive Surgery Discipline, School of Medicine, FMUSP - University of São Paulo, São Paulo, Brazil.
Robson Kiyoshi
RK
Ishida
1 Department of Gastroenterology, Digestive Surgery Discipline, School of Medicine, FMUSP - University of São Paulo, São Paulo, Brazil.
Marco Aurélio
MA
Santo
1 Department of Gastroenterology, Digestive Surgery Discipline, School of Medicine, FMUSP - University of São Paulo, São Paulo, Brazil.
Eduardo Guimarães Hourneaux
EG
de Moura
1 Department of Gastroenterology, Digestive Surgery Discipline, School of Medicine, FMUSP - University of São Paulo, São Paulo, Brazil.
Paulo
P
Sakai
1 Department of Gastroenterology, Digestive Surgery Discipline, School of Medicine, FMUSP - University of São Paulo, São Paulo, Brazil.
Ismael Francisco Mota Siqueira
IF
Guarda
2 UNIFESP - Federal University of São Paulo, São Paulo, Brazil.
Ismael Dale Cotrim Guerreiro
ID
da Silva
3 Statistics Department of Mathematics and Statistics Institute (EMI) - USP, São Paulo, Brazil.
Agatha Sacramento
AS
Rodrigues
3 Statistics Department of Mathematics and Statistics Institute (EMI) - USP, São Paulo, Brazil.
Carlos Alberto de Bragança
CA
Pereira
4 Pennington Biomedical Research Center, Baton Rouge, Louisiana, LA, USA.
Steven
S
Heymsfield
5 Institut National de la Recherche Agronomique INRA, France.
Joel
J
Doré
1 Department of Gastroenterology, Digestive Surgery Discipline, School of Medicine, FMUSP - University of São Paulo, São Paulo, Brazil.
Raquel Susana Matos de Miranda
RS
Torrinhas
6 University Nove de Julho, São Paulo, Brazil.
Daniel
D
Giannella-Neto
1 Department of Gastroenterology, Digestive Surgery Discipline, School of Medicine, FMUSP - University of São Paulo, São Paulo, Brazil.
Dan Linetzky
DL
Waitzberg
D000328:Adult; D001706:Biopsy; D003924:Diabetes Mellitus, Type 2 / Q000097:blood* / Q000150:complications / Q000601:surgery / Q000652:urine; D005243:Feces / Q000737:chemistry; D005247:Feeding Behavior; D005260:Female; D015390:Gastric Bypass; D041981:Gastrointestinal Tract / Q000378:metabolism* / Q000503:physiopathology / Q000601:surgery; D006801:Humans; D008137:Longitudinal Studies; D055442:Metabolome; D008875:Middle Aged; D009767:Obesity, Morbid / Q000097:blood* / Q000150:complications / Q000601:surgery / Q000652:urine; D011446:Prospective Studies; D020543:Proteome / Q000235:genetics / Q000378:metabolism*; D012074:Remission Induction; D012107:Research Design; D059467:Transcriptome; D015431:Weight Loss
D020543:Proteome
Roux-en-Y gastric bypass; faecal water; gene expression; lipidomic; metabolomic; metagenomic; microbiota; proteomic; type 2 diabetes mellitus