Expression of heparanase in basal cell carcinoma and squamous cell carcinoma.
Heparanase is an enzyme that cleaves heparan sulfate chains. Oligosaccharides generated by heparanase induce tumor progression. Basal cell carcinoma and squamous cell carcinoma comprise types of nonmelanoma skin cancer.
Evaluate the glycosaminoglycans profile and expression of heparanase in two human cell lines established in culture, immortalized skin keratinocyte (HaCaT) and squamous cell carcinoma (A431) and also investigate the expression of heparanase in basal cell carcinoma, squamous cell carcinoma and eyelid skin of individuals not affected by the disease (control).
Glycosaminoglycans were quantified by electrophoresis and indirect ELISA method. The heparanase expression was analyzed by quantitative RT-PCR (qRTPCR).
The A431 strain showed significant increase in the sulfated glycosaminoglycans, increased heparanase expression and decreased hyaluronic acid, comparing to the HaCaT lineage. The mRNA expression of heparanase was significantly higher in Basal cell carcinoma and squamous cell carcinoma compared with control skin samples. It was also observed increased heparanase expression in squamous cell carcinoma compared to the Basal cell carcinoma.
The glycosaminoglycans profile, as well as heparanase expression are different between HaCaT and A431 cell lines. The increased expression of heparanase in Basal cell carcinoma and squamous cell carcinoma suggests that this enzyme could be a marker for the diagnosis of such types of non-melanoma cancers, and may be useful as a target molecule for future alternative treatment.
Faculdade de Medicina do ABC (FMABC), Santo André, SP, Brazil.|Universidade Federal de São Paulo (UNIFESP), São Paulo, SP, Brazil.
Maria Aparecida Silva
MA
Pinhal
Universidade Federal de São Paulo (UNIFESP), São Paulo, SP, Brazil.
Maria Carolina Leal
MC
Almeida
Faculdade de Medicina do ABC (FMABC), Santo André, SP, Brazil.
Alessandra Scorse
AS
Costa
Faculdade de Medicina do ABC (FMABC), Santo André, SP, Brazil.|Universidade Federal de São Paulo (UNIFESP), São Paulo, SP, Brazil.
Thérèse Rachell
TR
Theodoro
Faculdade de Medicina do ABC (FMABC), Santo André, SP, Brazil.
Rodrigo Lorenzetti
RL
Serrano
Faculdade de Medicina do ABC (FMABC), Santo André, SP, Brazil.
Carlos D'Apparecida Santos
CD
Machado
D002280:Basal Cell Carcinoma / Q000201:enzymology*; D002294:Carcinoma, Squamous Cell / Q000201:enzymology*; D005143:Eyelids / Q000201:enzymology; D005966:Glucuronidase / Q000235:genetics / Q000378:metabolism*; D006025:Glycosaminoglycans / Q000032:analysis / Q000378:metabolism*; D006801:Humans; D006820:Hyaluronic Acid / Q000032:analysis / Q000378:metabolism; D015603:Keratinocytes / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods; D012878:Skin Neoplasms / Q000201:enzymology*; D000099175:Heparanase
D005966:Glucuronidase; D006025:Glycosaminoglycans; D006820:Hyaluronic Acid; D012333:RNA, Messenger; D000099175:Heparanase