Antidiabetic and Antihyperalgesic Effects of a Decoction and Compounds from Acourtia thurberi.
The purpose of this research was to examine the preclinical efficacy of a decoction from the roots ofAcourtia thurberias a hypoglycemic, antihyperglycemic, and antihyperalgesic agent using well-known experimental models in mice. Acute oral administration ofA. thurberidecoction did not produce toxic effects in mice, according to the Lorke procedure.A. thurberidecoction (31.6-316.2 mg/kg, p. o.) decreased blood glucose levels during acute hypoglycemic and the oral glucose tolerance and oral sucrose tolerance tests, both in normoglycemic and hyperglycemic animals. Phytochemical analysis ofA. thurberiroots led to the isolation of perezone (1), a mixture ofα-pipitzol (2) andβ-pipitzol (3), and 8-β-D-glucopyranosyloxy-4-methoxy-5-methyl-coumarin (4). A pharmacological evaluation of compounds1-4(3.2-31.6 mg/kg) using the same assays revealed their hypoglycemic and antihyperglycemic actions. Finally, local administration ofA. thurberidecoction (31.6-316.2 µg/paw) and compounds1-4(3.2-31.6 µg/paw) produced significant inhibition on the licking time during the formalin test in healthy and hyperglycemic mice, demonstrating their antinociceptive and antihyperalgesic potential, respectively. Altogether, these results could be related to the use ofA. thurberifor treating diabetes and painful complaints in contemporary Mexican folk medicine. A suitable UPLC-ESI/MS method was developed and successfully applied to quantify simultaneously compounds1and4inA. thurberidecoction.
Departamento de Farmacia, Facultad de Química, Universidad Nacional Autónoma de México, Ciudad de México, México.
Ana Laura
AL
Martínez
Departamento de Farmacia, Facultad de Química, Universidad Nacional Autónoma de México, Ciudad de México, México.
Abraham
A
Madariaga-Mazón
Departamento de Farmacia, Facultad de Química, Universidad Nacional Autónoma de México, Ciudad de México, México.
Isabel
I
Rivero-Cruz
Jardín Botánico, Instituto de Biología, Universidad Nacional Autónoma de México, Ciudad de México, México.
Robert
R
Bye
Departamento de Farmacia, Facultad de Química, Universidad Nacional Autónoma de México, Ciudad de México, México.
Rachel
R
Mata
D000818:Animals; D019659:Asteraceae / Q000737:chemistry*; D001786:Blood Glucose / Q000187:drug effects; D003921:Diabetes Mellitus, Experimental / Q000139:chemically induced / Q000188:drug therapy*; D004195:Disease Models, Animal; D006801:Humans; D007004:Hypoglycemic Agents / Q000302:isolation & purification* / Q000494:pharmacology*; D008297:Male; D008519:Medicine, Traditional; D008800:Mexico; D051379:Mice; D008813:Mice, Inbred ICR; D009536:Niacinamide / Q000009:adverse effects; D008517:Phytotherapy; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D010946:Plants, Medicinal; D012717:Sesquiterpenes / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D013311:Streptozocin / Q000009:adverse effects
D001786:Blood Glucose; D007004:Hypoglycemic Agents; D010936:Plant Extracts; D012717:Sesquiterpenes; C558405:pipitzol; D009536:Niacinamide; D013311:Streptozocin; C053752:perezone