Multispectroscopic studies on the interaction of a copper(ii) complex of ibuprofen drug with calf thymus DNA.
The interaction of copper(II)-ibuprofenato complex with calf thymus DNA (ct-DNA) has been explored following, UV-visible spectrophotometry, fluorescence measurement, dynamic viscosity measurements, and circular dichroism spectroscopy. In spectrophotometric studies of ct-DNA it was found that [Cu(ibp)2]2can form a complex with double-helical DNA. The association constant of [Cu(ibp)2]2with DNA from UV-Vis study was found to be 6.19 × 104L mol-1. The values of Kffrom fluorescence measurement clearly underscore the high affinity of [Cu(ibp)2]2to DNA. The experimental results showed that the conformational changes in DNA helix induced by [Cu(ibp)2]2are the reason for the fluorescence quenching of the DNA-Hoechst system. In addition, the fluorescence emission spectra of intercalated methylene blue (MB) with increasing concentrations of [Cu(ibp)2]2represented a significant increase of MB intensity as to release MB from MB-DNA system. The results of circular dichroism (CD) suggested that copper(II)-ibuprofenato complex can change the conformation of DNA. In addition, the results of viscosity measurements suggest that copper(II)-ibuprofenato complex may bind with non-classical intercalative mode. From spectroscopic and hydrodynamic studies, it has been found that [Cu(ibp)2]2interacts with DNA by partial intercalation mode which contains intercalation and groove properties.
a Inorganic Chemistry Department , Faculty of Chemistry, Razi University , Kermanshah , Iran.|b Medical Biology Research Center (MBRC) Kermanshah University of Medical Sciences , Kermanshah , Iran.
Nahid
N
Shahabadi
a Inorganic Chemistry Department , Faculty of Chemistry, Razi University , Kermanshah , Iran.
Farshad
F
Shiri
D001665:Binding Sites; D001667:Binding, Competitive; D006690:Bisbenzimidazole / Q000737:chemistry / Q000378:metabolism; D002942:Circular Dichroism; D004247:DNA / Q000737:chemistry / Q000378:metabolism*; D005453:Fluorescence; D007364:Intercalating Agents / Q000737:chemistry; D008751:Methylene Blue / Q000737:chemistry / Q000378:metabolism; D062105:Molecular Docking Simulation; D009690:Nucleic Acid Conformation; D009942:Organometallic Compounds / Q000737:chemistry* / Q000378:metabolism*; D013056:Spectrophotometry, Ultraviolet; D017550:Spectroscopy, Fourier Transform Infrared; D013816:Thermodynamics; D014783:Viscosity
D007364:Intercalating Agents; D009942:Organometallic Compounds; C418618:tetrakis(ibuprofenato)dicopper(II); D004247:DNA; C427203:calf thymus DNA; D006690:Bisbenzimidazole; D008751:Methylene Blue
Copper(II)-ibuprofen complex; DNA interaction; Spectral probes; partial intercalation