Identification of a discordant genotype at the D2S1338 locus between Identifiler Plus®16 kit and Power Plex®21 system kit.
Genotyping errors can perturb estimates of genetic diversity and adversely affects the reliability of forensic identification, which has become increasingly recognized. During our daily laboratory work, we hit upon a discordant short tandem repeat (STR) genotype at the D2S1338 locus between Identifiler Plus®16 system kit and Power Plex®21 system kit. To figure out the reason of the discordance, nested PCR was performed to amplify the D2S1338 locus and then the final amplicons were sequenced. We found a G to G/A transition at the position on which caused an allele dropout of Identifiler Plus®16 kit.
Department of Forensic Medicine, Xinxiang Medical University, Xinxiang, Henan, China.
Lin
L
Zhang
Department of Forensic Medicine, Xinxiang Medical University, Xinxiang, Henan, China.
Hongyan
H
Wu
Department of Forensic Medicine, Xinxiang Medical University, Xinxiang, Henan, China.
Kejie
K
Wang
Department of Forensic Medicine, Xinxiang Medical University, Xinxiang, Henan, China.
Aiying
A
Fan
Morphological Laboratory, Heze Medical College, Heze, Shandong, China.
Jiafeng
J
Niu
Department of Human Anatomy, Xinxiang Medical University, Xinxiang, Henan, China. Electronic address: zhuzhendong0413@163.com.
Zhendong
Z
Zhu
D002648:Child; D016172:DNA Fingerprinting; D005260:Female; D056426:Genetic Loci; D005838:Genotype; D006801:Humans; D018895:Microsatellite Repeats; D016133:Polymerase Chain Reaction / Q000295:instrumentation*
D2S1338; Forensic genetics; Null allele; Primer binding site mutation; STR