Therapeutic effect of erythroid differentiation regulator 1 (Erdr1) on collagen-induced arthritis in DBA/1J mouse.
Rheumatoid arthritis (RA) is a chronic inflammatory autoimmune disease, and multiple inflammatory cytokines are involved in RA pathogenesis. Interleukin (IL)-18, in particular, has a significant positive correlation with RA. In this study, we investigated the effect of erythroid differentiation regulator 1 (Erdr1), which is negatively regulated by IL-18, in an animal model of inflammatory arthritis, collagen-induced arthritis (CIA) in DBA/1J mice. Treatment of mice with recombinant (r)Erdr1 significantly suppressed the severity of arthritis, histologic features of arthritic tissue, and serum levels of anti-collagen autoantibodies (IgG, IgG1, IgG2a and IgM) in CIA. In addition, IL-18 expression was reduced in the affected synovium of rErdr1-treated mice. Interestingly, Erdr1 treatment suppressed migration in contrast to the pro-migratory effect of IL-18, indicating the therapeutic effects of Erdr1 on CIA through inhibiting synovial fibroblast migration. In addition, Erdr1 inhibited activation of ERK1/2, a key signaling pathway in migration of various cell types. Taken together, these data show that rErdr1 exerts therapeutic effects on RA by inhibiting synovial fibroblast migration, suggesting that rErdr1 treatment might be an effective therapeutic approach for RA.
Department of Cosmetic Sciences, Sookmyung Women's University, Yongsan-ku, Seoul, Republic of Korea.|Department of Biological Sciences, Sookmyung Women's University, Yongsan-ku, Seoul, Republic of Korea.
Kyung Eun
KE
Kim
Department of Biological Sciences, Sookmyung Women's University, Yongsan-ku, Seoul, Republic of Korea.
Sungryung
S
Kim
Department of Biological Sciences, Sookmyung Women's University, Yongsan-ku, Seoul, Republic of Korea.
Sunyoung
S
Park
Department of Biological Sciences, Sookmyung Women's University, Yongsan-ku, Seoul, Republic of Korea.
Younkyung
Y
Houh
Department of Plastic Surgery, Samsung Medical Center, Sungkyunkwan University School of Medicine, Gangnam-gu, Seoul, Republic of Korea.
Yoolhee
Y
Yang
Biotech Team, Central Research Institute, Ilyang Pharm. Co., Ltd., Gyeonggi-do, Republic of Korea.
Seung Beom
SB
Park
Biotech Team, Central Research Institute, Ilyang Pharm. Co., Ltd., Gyeonggi-do, Republic of Korea.
Sangyoon
S
Kim
Department of Anatomy, Inje University College of Medicine, Busan, Republic of Korea.
Daejin
D
Kim
Department of Anatomy, Inje University College of Medicine, Busan, Republic of Korea.
Dae Young
DY
Hur
Department of Anatomy, Inje University College of Medicine, Busan, Republic of Korea.
Seonghan
S
Kim
Department of Dermatology, Yeouido St. Mary's Hospital, The Catholic University of Korea, Seoul, Republic of Korea.
Hyun Jeong
HJ
Park
Department of Plastic Surgery, Samsung Medical Center, Sungkyunkwan University School of Medicine, Gangnam-gu, Seoul, Republic of Korea.
Sa Ik
SI
Bang
Department of Cosmetic Sciences, Sookmyung Women's University, Yongsan-ku, Seoul, Republic of Korea.|Department of Biological Sciences, Sookmyung Women's University, Yongsan-ku, Seoul, Republic of Korea.
Daeho
D
Cho
D000818:Animals; D001169:Arthritis, Experimental / Q000188:drug therapy / Q000209:etiology* / Q000378:metabolism / Q000473:pathology*; D001172:Arthritis, Rheumatoid / Q000188:drug therapy / Q000209:etiology / Q000473:pathology; D001323:Autoantibodies / Q000097:blood / Q000276:immunology; D003094:Collagen / Q000276:immunology; D004195:Disease Models, Animal; D015870:Gene Expression; D007074:Immunoglobulin G / Q000097:blood / Q000276:immunology; D007150:Immunohistochemistry; D020382:Interleukin-18 / Q000235:genetics / Q000378:metabolism; D008297:Male; D008565:Membrane Proteins / Q000494:pharmacology*; D051379:Mice; D008811:Mice, Inbred DBA; D011994:Recombinant Proteins; D012720:Severity of Illness Index; D025521:Tumor Suppressor Proteins / Q000494:pharmacology*
D001323:Autoantibodies; D007074:Immunoglobulin G; D020382:Interleukin-18; D008565:Membrane Proteins; D011994:Recombinant Proteins; D025521:Tumor Suppressor Proteins; C505963:erythroid differentiation regulator 1, mouse; D003094:Collagen
Immune response; Immunity; Immunology and Microbiology Section; erythroid differentiation regulator 1 (Erdr1); inflammation; interleukin-18 (IL-18); rheumatoid arthritis; synovial fibroblast migration