Mitochondrial DNA sequence-based phylogenetic relationship ofTrichiurus lepturus(Perciformes: Trichiuridae) from the Persian Gulf.
In this study, mitochondrial DNA analysis using 16S ribosomal DNA (rDNA) was performed to investigate the phylogeny relationship ofTrichiurus lepturusin the Persian Gulf compared to the other investigated area. The amplification of 16S rDNA resulted in a product of 600 bp in all samples. The results showed that the isolated strain belongs toT. lepturusshowing 42 divergence sites among the same reported partial sequences of 16S rRNA gene from the other area (West Atlantic and Indo-Pacific area). Phylogeny results showed that all 18 haplotypes of the species clustered into five clades with reasonably high bootstrap support of values (>64%). Overall, the tree topology for both phylogenetic and phenetic trees for 16S rDNA was similar. Both trees exposed two major clusters, one wholly containing the haplotypes of theT. lepturusspecies belonging to Indo-Pacific area with two major sister groups including Persian Gulf specimen and the other cleared the Western Atlantic and Japan individuals clustered in another distinct clade supporting the differentiation between the two areas. Phylogenic relationship observed between the Persian Gulf and the other Indo-Pacific Individuals suggested homogeneity between two mentioned areas.
Department of Genetic, Persian Gulf and Oman Sea Ecology Research Center, Iranian Fisheries Sciences Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Bandar Abass, Iran.
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Tamadoni Jahromi
Department of Biology, School of Biological Sciences, University Science Malaysia (USM), Penang, Malaysia.
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Mohd Noor
Department of Biotechnology, School of Agricultural Sciences, Bu-Alisina University, Hamadan, Iran.
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Pirian
Department of Biology, Persian Gulf and Oman Sea Ecology Research Center, Iranian Fisheries Sciences Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Bandar Abass, Iran.
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Dehghani
Department of Genetic, Persian Gulf and Oman Sea Ecology Research Center, Iranian Fisheries Sciences Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Bandar Abass, Iran.
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Nazemi
Young Researchers and Elite Club, Bandar Abass Branch, Islamic Azad University, Bandar Abass, Iran.
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Khazaali
16S rDNA; Mitochondrial DNA; Persian Gulf; Phylogeny; Trichiurus lepturus