Fever-range hyperthermia inhibits cells immune response to protein-bound polysaccharides derived from Coriolus versicolor extract.
The aim of the study was to explore whether fever-range hyperthermia (FRH) might enhance the anticancer and immunoregulatory activities of protein-bound polysaccharides (PBP), a class of fungus derived immunomodifiers used in the cancer adjuvant therapy. Blood lymphocytes and breast cancer cells (MCF-7) were cultured at 39.5°C in humidified atmosphere containing 5% CO2for 2h. After rested at 37°C for 6h, the cells were treated with PBP extract at 100- and 300μg/ml concentration. After indicated time, the proliferative response was analyzed and cytokine mRNA expression assessment was performed by qRT-PCR. In animal model, the FRH was induced by placing rats in the Homeothermic Controller with heating blanket. Animals were heated until Tb reached 39.5°C (±0.2°C) and were maintained at this temperature for 30min. The protein-bound polysaccharides solution was injected i.p. at a dose of 100 mg/kg 6h post FRH. Twenty four hours after treatment, the blood was collected and cytokines expression analysis were performed. The results have shown that fever-range hyperthermia has an inhibitory effect on PBP extract-induced proliferative response of blood lymphocytes, as well as IL-1β and IL-6 mRNA expression. Moreover, the temperature of 39.5°C blocks PBP-induced cytotoxicity against MCF-7 cells, which correlates with significant reduction in TNF-α level. Combined treatment of rats (FRH+PBP) results in decrease of IL-1β, IL-6 and TNF-α mRNA expression in peripheral blood mononuclear cells compared to cells derived from rats treated with protein-bound polysaccharides extract alone. This study demonstrates that fever-range temperature inhibits immunostimulatory as well as anticancer effects mediated by protein-bound polysaccharides.
Department of Immunology, Faculty of Biology and Environment Protection, Nicolaus Copernicus University, Lwowska 1 Street, 87-100 Torun, Poland. Electronic address: m_pawlikowska@umk.pl.
Małgorzata
M
Pawlikowska
Department of Immunology, Faculty of Biology and Environment Protection, Nicolaus Copernicus University, Lwowska 1 Street, 87-100 Torun, Poland. Electronic address: tomaszj@umk.pl.
Tomasz
T
Jędrzejewski
Department of Immunology, Faculty of Biology and Environment Protection, Nicolaus Copernicus University, Lwowska 1 Street, 87-100 Torun, Poland. Electronic address: piotrowski_jak@umk.pl.
Jakub
J
Piotrowski
Department of Immunology, Faculty of Biology and Environment Protection, Nicolaus Copernicus University, Lwowska 1 Street, 87-100 Torun, Poland. Electronic address: wkozak@umk.pl.
Wiesław
W
Kozak
D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects; D016207:Cytokines / Q000096:biosynthesis; D005260:Female; D005656:Fungal Proteins / Q000494:pharmacology*; D006801:Humans; D006979:Hyperthermia, Induced; D008214:Lymphocytes / Q000187:drug effects / Q000276:immunology*; D061986:MCF-7 Cells; D023421:Models, Animal; D016133:Polymerase Chain Reaction; D011134:Polysaccharides / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D060888:Real-Time Polymerase Chain Reaction
D000276:Adjuvants, Immunologic; D016207:Cytokines; D005656:Fungal Proteins; D011134:Polysaccharides
Coriolus versicolor; Cytokine; Fever-range hyperthermia; Lymphocytes; MCF-7 cells; Protein-bound polysaccharides