Preparation, textural and photoluminescence characterization of green fluorescence protein-immobilised Ga-ZnO (GZO)-nanocomposites.
Nanostructured thin films of Gallium doped Zinc oxide (GZO) with nanodisk/nanorods and nanoflower morphologies are fabricated on a piezoelectric substrate. Pure wurtzite phase of GZO with nanostructure morphologies were prepared by a one-step spin coating process, followed by hydrothermal treatment. A non-ionic polymer (polyethylene imine) was used as a structure assisting agent to selectively form the nanodisks or nanoflowers depending on the reaction conditions. The morphology, nanostructure, and physicochemical properties of GZO were studied by X-ray diffraction, scanning electron microscopy (SEM) and Raman spectroscopy. The thicknesses and lengths of the individual GZO nanodisks were measured by FE-SEM. The fine nanodisk and nanoflower structures are obtained. Green fluorescent proteins were immobilised on the as-synthesised GZO nanostructured materials by dip coating. Atomic force microscopy was used to study the surface roughness of the GZO nanodisks, nanoflowers and nanorods. Photoluminescence techniques were used to study the GZO nanodisk structural defects and its optical properties. Fluorescence spectrometry analyses confirmed the binding of green fluorescent protein on the GZO nanostructure surface. The biocompatibility study of GZO nanostructures have been studied using Human HT-29 colon cell lines. Trace levels of green florescent protein immobilised on Ga-doped ZnO nanodisks and nanoflowers showed good activity for UV light sensing.
Surfactants Research Chair, Chemistry Department, College of Science, King Saud University, P.O.Box. 2455, Riyadh 11451, Saudi Arabia; School of Engineering, University of Ulsan, Ulsan, South Korea. Electronic address: rjothiram@gmail.com.
Jothi Ramalingam
JR
Rajabathar
Department of Botany and Microbiology, College of Science, King Saud University, Riyadh 11451, Saudi Arabia.
Murugan A
MA
Munusamy
Surfactants Research Chair, Chemistry Department, College of Science, King Saud University, P.O.Box. 2455, Riyadh 11451, Saudi Arabia.
Hamad A
HA
Al-Lohedan
D005708:Gallium / Q000737:chemistry*; D049452:Green Fluorescent Proteins / Q000737:chemistry*; D049449:Luminescence; D053768:Metal Nanoparticles / Q000737:chemistry*; D018625:Microscopy, Atomic Force; D008855:Microscopy, Electron, Scanning; D015034:Zinc Oxide / Q000737:chemistry*
D049452:Green Fluorescent Proteins; D005708:Gallium; D015034:Zinc Oxide
Gallium; Green fluorescence protein; Human HT-29; Nanoflowers; Sensors; ZnO nanodisk; ZnO thinfilm