Facile Preparation of Photoactivatable Surfaces with Tuned Substrate Adhesiveness.
This paper describes a facile method for the preparation of photoactivatable substrates with tuned surface density of an extracellular matrix peptide to resolve the impacts of biochemical and mechanical cues on collective cell migration. The controllability of surface ligand density was validated by cell adhesion and migration tests, complemented with fluorescence observation of an alternative ligand. Depending on the surface ligand density, HeLa cells either kept or lost collective characteristics. The present materials will be useful to address mechanobiology of collective cell migration.
WPI Research Initiative for Materials Nanoarchitectonics (MANA), National Institute for Materials Science (NIMS).
Yoshihisa
Y
Shimizu
Masao
M
Kamimura
Shota
S
Yamamoto
Shimaa A
SA
Abdellatef
Kazuo
K
Yamaguchi
Jun
J
Nakanishi
D000268:Adhesiveness; D002448:Cell Adhesion; D002450:Cell Communication; D002465:Cell Movement; D004220:Disulfides / Q000737:chemistry; D005109:Extracellular Matrix / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D008024:Ligands; D010455:Peptides / Q000737:chemistry; D010777:Photochemistry / Q000379:methods*; D013499:Surface Properties
D004220:Disulfides; D008024:Ligands; D010455:Peptides