Analysis of polyethylene glycol (PEG) fusion in cultured neuroblastoma cells via flow cytometry: Techniques & optimization.
Polyethylene glycol (PEG) has long been used as a membrane fusogen, but recently it has been adopted as a technique for peripheral nerve repair. Vertebrate models using PEG fusion have shown improved outcomes when PEG is applied during repair of severed peripheral nerves. The cellular mechanism of PEG fusion in the peripheral nerve repair model has not previously been assessed via flow cytometry. PEG fusion was assessed in this experiment by dying B35 rat neuroblastoma cells with different color fluorescent labels. The different color cells were combined and PEG was applied in concentrations of 50%, 75% and 100%. The amount of cell fusion was assessed via flow cytometry as the percentage of double positive cells. Results showed increasing fusion and decreasing viability with increasing concentrations of PEG.
Vanderbilt School of Medicine, 1161 21st Ave S # T1217, Nashville, TN 37232, United States. Electronic address: Ashley.n.hoffman@vanderbilt.edu.
Ashley N
AN
Hoffman
Department of Plastic Surgery, Vanderbilt University Medical Center, 1161 21st Ave South, MCN S-2221, Nashville, TN, United States. Electronic address: rbamba@gmail.com.
Ravinder
R
Bamba
Department of Plastic Surgery, Vanderbilt University Medical Center, 1161 21st Ave South, MCN S-2221, Nashville, TN, United States. Electronic address: Alonda.c.pollins@vanderbilt.edu.
Alonda C
AC
Pollins
Department of Plastic Surgery, Vanderbilt University Medical Center, 1161 21st Ave South, MCN S-2221, Nashville, TN, United States. Electronic address: Wesley.thayer@vanderbilt.edu.
Wesley P
WP
Thayer
D000818:Animals; D002459:Cell Fusion / Q000379:methods; D045744:Cell Line, Tumor; D005434:Flow Cytometry / Q000379:methods*; D009447:Neuroblastoma / Q000473:pathology; D009474:Neurons / Q000187:drug effects*; D011092:Polyethylene Glycols / Q000494:pharmacology*; D051381:Rats
D011092:Polyethylene Glycols
Flow cytometry; Neuroblastoma cells; PEG fusion; Peripheral nerve repair; Polyethylene glycol