The polymorphisms of methionine synthase (MTR) and methionine synthase reductase (MTRR) genes in pathogenesis of preeclampsia.
OBJECTIVE
The aim of the study was to determine the MTR (methionine synthase) and MTRR (mehionine synthase reductase) polymorphisms in pregnant women with preeclampsia (PE).


METHODS
The group of 98 women with PE and the group of 120 healthy pregnant women were analyzed. Determination of MTR 2756A > G and MTRR 66A > G polymorphisms was performed using polymerase chain reaction (PCR)/restriction fragment length polymorphism (RFLP) method.


RESULTS
The study did not show any statistically significant differences in frequency of genotypes and alleles of MTR 2756A > G polymorphism between PE group and controls. Higher frequency of 66GG genotype and 66G allele of MTRR 66A > G polymorphism was observed in the women with PE compared to control group. Moreover, the 66GG genotype correlated with higher doses of methyldopa, lower birth weight and higher placenta weight in women with PE.


CONCLUSIONS
The obtained results for 66A > G polymorphism of MTRR gene suggest the predisposition to PE in carriers of mutated 66GG genotype and - 66G allele.
a Division of Perinatology and Women's Diseases, Poznan University of Medical Sciences , Poznan , Poland.|b Department of Pharmacology and Phytochemistry.
Agnieszka
A
Seremak-Mrozikiewicz
c Department of Stem Cells and Regenerative Medicine, Institute of Natural Fibres and Medicinal Plants , Poznan , Poland.|d Department of Histocompatibility with Laboratory of Genetic Diagnostics, Regional Blood Center , Poznan , Poland.
Anna
A
Bogacz
a Division of Perinatology and Women's Diseases, Poznan University of Medical Sciences , Poznan , Poland.
Donata
D
Deka-Pawlik
e Department of Nursing, Poznan University of Medical Sciences , Poznan , Poland.
Andrzej
A
Klejewski
a Division of Perinatology and Women's Diseases, Poznan University of Medical Sciences , Poznan , Poland.|f Division of Gynecology and Obstetrics, Podhale Multidisciplinary Hospital , Nowy Targ , Poland.
Hubert
H
Wolski
a Division of Perinatology and Women's Diseases, Poznan University of Medical Sciences , Poznan , Poland.
Krzysztof
K
Drews
c Department of Stem Cells and Regenerative Medicine, Institute of Natural Fibres and Medicinal Plants , Poznan , Poland.
Monika
M
Karasiewicz
c Department of Stem Cells and Regenerative Medicine, Institute of Natural Fibres and Medicinal Plants , Poznan , Poland.|g Department of General Pharmacology and Pharmacoeconomics, Pomeranian Medical University , Szczecin , Poland.
Boguslaw
B
Czerny
D013766:5-Methyltetrahydrofolate-Homocysteine S-Methyltransferase / Q000235:genetics*; D000328:Adult; D016022:Case-Control Studies; D005260:Female; D005287:Ferredoxin-NADP Reductase / Q000235:genetics*; D006801:Humans; D011110:Polymorphism, Genetic; D011225:Pre-Eclampsia / Q000235:genetics*; D011247:Pregnancy; D055815:Young Adult
C111241:methionine synthase reductase; D005287:Ferredoxin-NADP Reductase; D013766:5-Methyltetrahydrofolate-Homocysteine S-Methyltransferase
Preeclampsia; mehionine synthase reductase; methionine synthase; polymorphism