DNA-PAINT Super-Resolution Imaging for Nucleic Acid Nanostructures.
Far-field super-resolution fluorescence microscopy has allowed observation of biomolecular and synthetic nanoscale systems with features on the nanometre scale, with chemical specificity and multiplexing capability. DNA-PAINT (DNA-based point accumulation for imaging in nanoscale topography) is a super-resolution method that exploits programmable transient hybridization between short oligonucleotide strands, and allows multiplexed, single-molecule, single-label visualization with down to ~5-10 nm resolution. DNA-PAINT provides a method for structural characterisation of nucleic acid nanostructures with high spatial resolution and single-strand visibility.
Wyss Institute for Biologically Inspired Engineering, Harvard University, CLSB 5th Floor, Blackfan Circle, Boston, MA, 02115, USA. mingjie.dai@wyss.harvard.edu.|Program in Biophysics, Harvard University, Boston, MA, 02115, USA. mingjie.dai@wyss.harvard.edu.
Mingjie
M
Dai
D004247:DNA / Q000737:chemistry*; D008856:Microscopy, Fluorescence / Q000379:methods; D049329:Nanostructures / Q000737:chemistry*; D036103:Nanotechnology / Q000379:methods*; D009696:Nucleic Acids / Q000737:chemistry*; D009841:Oligonucleotides / Q000737:chemistry
D009696:Nucleic Acids; D009841:Oligonucleotides; D004247:DNA
DNA-PAINT; Fluorescence microscopy; Super-resolution imaging