Genome-wide expression analysis of a rice mutant line under salt stress.
Salinity is a major environmental stress to plants. In this study, the ability of plants to tolerate salt was investigated by studying growth, physiological characteristics, and expression levels of genes related to the salt-stress response in the salt-tolerant rice mutant (Till-II-877), which was derived from γ-ray irradiation. Compared to plants grown under normal conditions, the height and root length of wild type (WT) were reduced by approximately 40 and 29% following exposure to salt stress for 3 weeks, whereas Till-II-877 line showed 29 and 23% reductions in plant height and root length, respectively. No significant changes were observed in total chlorophyll content, and the malondialdehyde content of the mutant increased less than that of the WT under salt treatment. Gene expression was compared between the WT and mutant lines using microarray analysis. An unbiased analysis of the gene expression datasets allowed us to identify the pathways involved in salt-stress responses. Among the most significantly affected pathways, changes in gene expression were observed in α-linolenic acid and linoleic acid metabolism (in lipid metabolism), fructose and mannose metabolism and glycolysis-gluconeogenesis (in carbohydrate metabolism), cysteine and methionine metabolism (in amino acid metabolism), and carbon fixation (in the energy metabolism of photosynthetic organisms) under salt stress. These results show that the differential response of plants subjected to salt stress was due to changes in multiple metabolic pathways. These findings increase our understanding of the effects of salt stress in rice and may aid in the development of salt-tolerant rice cultivars.
National Agrobiodiversity Center, National Academy of Agricultural Science, Rural Development Administration, Jeonju, Jeonbuk, Korea.
K J
KJ
Lee
Radiation Breeding Research Center, Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, Jeongeup, Jeonbuk, Korea.|Radiation Biotechnology and Applied Radioisotope Science, University of Science and Technology, Daejeon, Korea.
S-J
SJ
Kwon
Radiation Breeding Research Center, Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, Jeongeup, Jeonbuk, Korea.
J E
JE
Hwang
Radiation Breeding Research Center, Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, Jeongeup, Jeonbuk, Korea.
S M
SM
Han
Radiation Breeding Research Center, Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, Jeongeup, Jeonbuk, Korea.
I
I
Jung
Radiation Breeding Research Center, Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, Jeongeup, Jeonbuk, Korea.
J-B
JB
Kim
Radiation Breeding Research Center, Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, Jeongeup, Jeonbuk, Korea.
H-I
HI
Choi
Radiation Breeding Research Center, Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, Jeongeup, Jeonbuk, Korea jhryu@kaeri.re.kr.
J
J
Ryu
Radiation Breeding Research Center, Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, Jeongeup, Jeonbuk, Korea jhryu@kaeri.re.kr.
S-Y
SY
Kang
D002338:Carotenoids / Q000378:metabolism; D002734:Chlorophyll / Q000378:metabolism; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D063990:Gene Ontology; D017343:Genes, Plant; D018745:Genome, Plant; D008315:Malondialdehyde / Q000378:metabolism; D053858:Metabolic Networks and Pathways / Q000187:drug effects / Q000235:genetics; D009154:Mutation / Q000235:genetics*; D012275:Oryza / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development / Q000502:physiology*; D012965:Sodium Chloride / Q000494:pharmacology; D013312:Stress, Physiological / Q000187:drug effects / Q000235:genetics*; D014158:Transcription, Genetic / Q000187:drug effects
D002734:Chlorophyll; D002338:Carotenoids; D012965:Sodium Chloride; D008315:Malondialdehyde