Abrasive Endoprosthetic Wear Particles Inhibit IFN-γ Secretion in Human Monocytes Via Upregulating TNF-α-Induced miR-29b.
The adverse biological responses to prostheses wear particles commonly led to the failure of total hip arthroplasty. Among the released cytokines, interferon-γ (IFN-γ) has been found to be a critical functional factor during osteoclast differentiation. However, the molecular mechanism underlying the regulation of IFN-γ in wear particles-induced cells still needs to be determined. Four kinds of abrasive endoprosthetic wear particle were used to treat THP-1 cells, including polymethylmethacrylate (PMMA), zirconiumoxide (ZrO2), commercially pure titanium (cpTi), and titanium alloy (Ti-6Al-7Nb), with a concentration of 0.01, 0.05, 0.1, or 0.2 mg/ml for 48 h. The expression of IFN-γ and miR-29b was detected by real-time RT-PCR or ELISA. Luciferase reporter assay was performed to determine the regulation of miR-29b on IFN-γ. The effect of miR-29b inhibitor on the expression of wear particle-induced IFN-γ was detected. The expression of miR-29b was examined in THP-1 cells treated with tumor necrosis factor-alpha (TNF-α). The expression of IFN-γ was downregulated and the level of miR-29b was increased in THP-1 cells pretreated with wear particles. IFN-γ was a target of miR-29b. Wear particles inhibited the expression of IFN-γ through miR-29b. The expression of miR-29b was significantly reduced in THP-1 cells treated with TNF-α neutralizing antibody and particles comparing to that in the cells treated with particles alone. Wear particles inhibit the IFN-γ secretion in human monocytes, which was associated with the upregulating TNF-α-induced miR-29b.
Department of Orthopedics, Tianjin Hospital, No. 406, Jie Fang South Rd, Tianjin, 300211, People's Republic of China.
Yan-Min
YM
Bu
Department of Orthopedics, Tianjin Hospital, No. 406, Jie Fang South Rd, Tianjin, 300211, People's Republic of China.
De-Zhi
DZ
Zheng
Department of Orthopedics, Tianjin Hospital, No. 406, Jie Fang South Rd, Tianjin, 300211, People's Republic of China.
Lei
L
Wang
Department of Orthopedics, Tianjin Hospital, No. 406, Jie Fang South Rd, Tianjin, 300211, People's Republic of China. Ljjunliu@126.com.
Jun
J
Liu
D002460:Cell Line; D002478:Cells, Cultured; D005786:Gene Expression Regulation; D006801:Humans; D007371:Interferon-gamma / Q000037:antagonists & inhibitors / Q000378:metabolism*; D007595:Joint Prosthesis / Q000009:adverse effects; D035683:MicroRNAs / Q000502:physiology*; D009000:Monocytes / Q000378:metabolism*; D052638:Particulate Matter / Q000737:chemistry / Q000494:pharmacology*; D019904:Polymethyl Methacrylate; D011475:Prosthesis Failure / Q000209:etiology*; D014025:Titanium; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism*; D015854:Up-Regulation; D015040:Zirconium
C532045:MIRN29a microRNA, human; D035683:MicroRNAs; D052638:Particulate Matter; D014409:Tumor Necrosis Factor-alpha; D007371:Interferon-gamma; D019904:Polymethyl Methacrylate; D015040:Zirconium; D014025:Titanium; C028541:zirconium oxide
IFN-γ; TNF-α; abrasive endoprosthetic wear particles; human monocytes; miR-29b