Prevalence of Extended-Spectrum β-Lactamases CTX-M-8 and CTX-M-2-Producing Salmonella Serotypes from Clinical and Nonhuman Isolates in Brazil.
We characterized extended-spectrum β-lactamases (ESBL) enzymes among Salmonella strains isolated in Brazil from 2009 to 2014. Salmonella recovered from both clinical and nonhuman (food, poultry, and environment) sources were subjected to antimicrobial susceptibility testing. β-lactamases genes were detected by polymerase chain reaction/sequencing; plasmid profiles and transferability were assessed by S1-pulsed field gel electrophoresis (PFGE). Genetic diversity was evaluated by XbaI-PFGE. Out of 630 Salmonella strains screened, 46 displayed ESBL phenotype, distributed across 11 different serotypes. blaCTX-M-8and blaCTX-M-2genes were detected at frequencies of 47% and 41%, respectively. blaSHV-5and blaSHV-2were also detected but in lower frequencies (4%, 2%). blaTEM-1gene was detected in 22% of the strains. Most of the ESBL genes were transferable by conjugation, and the respective blaESBLgene was detected in the recipient strain, indicating the location of ESBL determinants on transferable plasmids. XbaI-PFGE revealed genomic diversity of Salmonella Typhimurium bearing blaCTX-M-2, blaCTX-M-8, blaTEM-1, and blaSHV-2genes. Salmonella Muenchen (harboring blaCTX-M-2) and Salmonella Corvallis (blaCTX-M-8and blaSHV-5) showed clonal relatedness within respective serotypes. Our findings underscore the occurrence of diverse ESBL genes in several Salmonella serotypes, reinforcing the need for continuous surveillance of resistance genes circulating in human and nonhuman sources.
1 Centro de Bacteriologia , Instituto Adolfo Lutz, São Paulo, Brazil .
Sueli Aparecida
SA
Fernandes
1 Centro de Bacteriologia , Instituto Adolfo Lutz, São Paulo, Brazil .
Carlos Henrique
CH
Camargo
1 Centro de Bacteriologia , Instituto Adolfo Lutz, São Paulo, Brazil .
Gabriela Rodrigues
GR
Francisco
1 Centro de Bacteriologia , Instituto Adolfo Lutz, São Paulo, Brazil .
Maria Fernanda Campagnari
MFC
Bueno
1 Centro de Bacteriologia , Instituto Adolfo Lutz, São Paulo, Brazil .
Doroti Oliveira
DO
Garcia
2 Division of Infectious Diseases, University of Pittsburgh School of Medicine , Pittsburgh, Pennsylvania.
Yohei
Y
Doi
1 Centro de Bacteriologia , Instituto Adolfo Lutz, São Paulo, Brazil .
Monique Ribeiro Tiba
MRT
Casas
D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001938:Brazil / Q000453:epidemiology; D002645:Chickens; D003227:Conjugation, Genetic; D024901:Drug Resistance, Multiple, Bacterial / Q000235:genetics*; D016521:Electrophoresis, Gel, Pulsed-Field; D015870:Gene Expression; D022761:Gene Transfer, Horizontal; D014644:Genetic Variation; D006801:Humans; D007527:Isoenzymes / Q000235:genetics / Q000378:metabolism; D008461:Meat Products / Q000382:microbiology; D008826:Microbial Sensitivity Tests; D010957:Plasmids / Q000737:chemistry / Q000378:metabolism; D015995:Prevalence; D062486:Public Health Surveillance; D012475:Salmonella / Q000145:classification / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification; D012480:Salmonella Infections / Q000188:drug therapy / Q000453:epidemiology* / Q000382:microbiology; D012486:Salmonella typhimurium / Q000145:classification / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification; D065288:Serogroup; D012722:Sewage / Q000382:microbiology; D001618:beta-Lactamases / Q000235:genetics* / Q000378:metabolism
D000900:Anti-Bacterial Agents; D007527:Isoenzymes; D012722:Sewage; C071167:beta-lactamase CTX-2; D001618:beta-Lactamases
ESBL; microbial drug resistance; public health