Clinical significance of GCF sIL-6R and calprotectin to evaluate the periodontal inflammation.
Background Periodontitis is an inflammatory disease. The aim of this study was to investigate whether the soluble form of interleukin-6 receptor (sIL-6R) and calprotectin concentrations in gingival crevicular fluid are useful biomarkers in the evaluation of periodontitis. Methods First, a cross-sectional study was performed. A total of 34 periodontitis patients were enrolled and the gingival crevicular fluid samples were collected from the healthy and inflamed sites of periodontal pockets in each patient. The relationship between periodontal condition and gingival crevicular fluid sIL-6R and calprotectin concentrations was analysed statistically. The cut-off values of gingival crevicular fluid sIL-6R and calprotectin concentrations for the evaluation of periodontitis were determined using a receiver operating characteristic curve. Next, by using enzyme-linked immunosorbent assay, it was examined whether calprotectin induces sIL-6R production in THP-1 macrophages. Results Both gingival crevicular fluid sIL-6R and calprotectin concentrations were significantly higher in the inflamed sites than in the healthy sites ( P < 0.0001). The cut-off values of gingival crevicular fluid sIL-6R and calprotectin concentrations for the evaluation of periodontal inflammation were as follows: sIL-6R: 43.5 pg/site; calprotectin: 134.3 ng/site. In the in vitro study, calprotectin significantly induced sIL-6R production in THP-1 macrophages ( P < 0.01). Conclusions Both gingival crevicular fluid sIL-6R and calprotectin concentrations are significant biomarkers in the evaluation of periodontal inflammation.
Department of Periodontology and Endodontology, Institute of Health Biosciences, Tokushima University Graduate School, Tokushima, Japan.
Yukari
Y
Kajiura
Department of Periodontology and Endodontology, Institute of Health Biosciences, Tokushima University Graduate School, Tokushima, Japan.
Jung-Hwan
JH
Lew
Department of Periodontology and Endodontology, Institute of Health Biosciences, Tokushima University Graduate School, Tokushima, Japan.
Takahisa
T
Ikuta
Department of Periodontology and Endodontology, Institute of Health Biosciences, Tokushima University Graduate School, Tokushima, Japan.
Yasufumi
Y
Nishikawa
Department of Periodontology and Endodontology, Institute of Health Biosciences, Tokushima University Graduate School, Tokushima, Japan.
Jun-Ichi
JI
Kido
Department of Periodontology and Endodontology, Institute of Health Biosciences, Tokushima University Graduate School, Tokushima, Japan.
Toshihiko
T
Nagata
Department of Periodontology and Endodontology, Institute of Health Biosciences, Tokushima University Graduate School, Tokushima, Japan.
Koji
K
Naruishi
D000368:Aged; D015415:Biomarkers / Q000378:metabolism; D018450:Disease Progression; D005260:Female; D005883:Gingival Crevicular Fluid / Q000378:metabolism*; D006801:Humans; D039841:Leukocyte L1 Antigen Complex / Q000378:metabolism*; D008264:Macrophages / Q000378:metabolism; D008297:Male; D010518:Periodontitis / Q000276:immunology / Q000378:metabolism*; D019947:Receptors, Interleukin-6 / Q000096:biosynthesis / Q000737:chemistry* / Q000378:metabolism*; D012995:Solubility
D015415:Biomarkers; D039841:Leukocyte L1 Antigen Complex; D019947:Receptors, Interleukin-6
Periodontitis; calprotectin; gingival crevicular fluid; laboratory test; sIL-6R