Comparison of chito-oligosaccharide production from three different colloidal chitosans using the endochitonsanolytic system of Bacillus thuringiensis.
Bacillus thuringiensis is a nonhuman pathogen bacterium that is used as a fungal and insect biocontrol agent. Because of its environmental interaction, it possesses several extracellular enzymes that are able to degrade chitin and chitosan, two of the most important polymers because of their application in numerous fields. However, in recent years, it has been observed that oligosaccharides from the enzymatic degradation of chitosan have important benefits for human health. Comparison and exploration of the production of chito-oligosaccharides from different sources of chitosan will improve the process parameters and expand the biotechnology based in these molecules. This study shows the production of chito-oligosaccharides from three different sources of colloidal chitosan and conducts a qualitative-quantitative comparison between them, using the extracellular enzyme of B. thuringiensis. We found that in the three substrates, it is possible to get a mixture of chito-oligosaccharides from dimer to hexamer in a concentration range from 0.72 to 8.09 mg · g-1of original substrate. The best substrate to obtain these molecules was commercial chitosan as it has the highest production yields.
a Department of Microbiology, ENCB-IPN, Col Santo Tomas , Delegación Miguel Hidalgo , México.
Dario Rafael
DR
Olicón-Hernández
b CICATA-IPN Querétaro, Col Colinas del Cimatario , Querétaro , México.
Pedro Alberto
PA
Vázquez-Landaverde
a Department of Microbiology, ENCB-IPN, Col Santo Tomas , Delegación Miguel Hidalgo , México.
Ramón
R
Cruz-Camarillo
c Facultad de Ciencias Biológicas y Agropecuarias, Universidad Veracruzana, Municipio de Amatlán de Los Reyes , México.
Luz Irene
LI
Rojas-Avelizapa
D001413:Bacillus thuringiensis / Q000378:metabolism*; D048271:Chitosan / Q000737:chemistry* / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D003102:Colloids / Q000737:chemistry*; D004798:Enzymes / Q000378:metabolism; D009844:Oligosaccharides / Q000096:biosynthesis*
D003102:Colloids; D004798:Enzymes; D009844:Oligosaccharides; D048271:Chitosan
Bacillus thuringiensis; chitin; chito-oligosaccharides; chitosan; chitosanolytic activity; shrimp waste