A promising magnetic SERS immunosensor for sensitive detection of avian influenza virus.
Avian influenza viruses infect a great number of global populations every year and can lead to severe epidemics with high morbidity and mortality. Facile, rapid and sensitive detection of viruses is very crucial to control the viral spread at its early stage. In this work, we developed a novel magnetic immunosensor based on surface enhanced Raman scattering (SERS) spectroscopy to detect intact but inactivated influenza virus H3N2 (A/Shanghai/4084T/2012) by constructing a sandwich complex consisting of SERS tags, target influenza viruses and highly SERS-active magnetic supporting substrates. The procedure of sample pretreatment could be significantly simplified since the magnetic supporting substrates allowed the enrichment and separation of viruses from a complex matrix. With a portable Raman spectrometer, the immunosensor could detect H3N2 down to 102TCID50/mL (TCID50refers to tissue culture infection dose at 50% end point), with a good linear relationship from 102to 5×103TCID50/mL. Considering its time efficiency, portability and sensitivity, the proposed SERS-based magnetic immunoassay is very promising for a point-of-care (POC) test in clinical and diagnostic praxis.
Department of Infectious Diseases, Shanghai Public Health Clinical Center, Fudan University, Shanghai 201508, China; Institute of Arthritis Research, Guanghua Integrative Medicine Hospital, Shanghai 200052, China; Shanghai TargetDrug Ltd., Shanghai 201202, China.
Yang
Y
Sun
The Education Ministry Key Lab of Resource Chemistry, Shanghai Key Laboratory of Rare Earth Functional Materials, Shanghai Municipal Education Committee Key Laboratory of Molecular Imaging Probes and Sensors and Department of Chemistry, Shanghai Normal University, Shanghai 200234, China.
Li
L
Xu
Department of Infectious Diseases, Shanghai Public Health Clinical Center, Fudan University, Shanghai 201508, China.
Fengdi
F
Zhang
Department of Pathogen Diagnosis and Biosafety, Shanghai Public Health Clinical Center, Fudan University, Shanghai 201508, China.
Zhigang
Z
Song
Department of Pathogen Diagnosis and Biosafety, Shanghai Public Health Clinical Center, Fudan University, Shanghai 201508, China.
Yunwen
Y
Hu
Department of Infectious Diseases, Shanghai Public Health Clinical Center, Fudan University, Shanghai 201508, China.
Yongjia
Y
Ji
Department of Infectious Diseases, Shanghai Public Health Clinical Center, Fudan University, Shanghai 201508, China.
Jiayin
J
Shen
Shanghai TargetDrug Ltd., Shanghai 201202, China.
Ben
B
Li
Department of Infectious Diseases, Shanghai Public Health Clinical Center, Fudan University, Shanghai 201508, China. Electronic address: luhongzhou@fudan.edu.cn.
Hongzhou
H
Lu
The Education Ministry Key Lab of Resource Chemistry, Shanghai Key Laboratory of Rare Earth Functional Materials, Shanghai Municipal Education Committee Key Laboratory of Molecular Imaging Probes and Sensors and Department of Chemistry, Shanghai Normal University, Shanghai 200234, China. Electronic address: hfyang@shnu.edu.cn.
Haifeng
H
Yang
D000818:Animals; D015374:Biosensing Techniques; D001717:Birds / Q000821:virology; D002681:China; D006046:Gold / Q000737:chemistry; D006801:Humans; D007118:Immunoassay; D053122:Influenza A Virus, H3N2 Subtype / Q000302:isolation & purification* / Q000472:pathogenicity; D005585:Influenza in Birds / Q000175:diagnosis / Q000821:virology*; D007251:Influenza, Human / Q000175:diagnosis / Q000821:virology*; D008280:Magnetics; D013059:Spectrum Analysis, Raman
D006046:Gold
Immunosensor; Influenza virus; Magnetic separation; Sandwich complex; Surface enhanced Raman scattering