Monitoring of dynamin during the Toxoplasma gondii cell cycle.
The obligate intracellular protozoan parasite Toxoplasma gondii actively invades virtually all warm-blooded nucleated cells. This process results in a non-fusogenic vacuole, inside which the parasites replicate continuously until egress signaling is triggered. In this work, we investigated the role of the large GTPase dynamin in the interaction of T. gondii with the host cell by using laser and electron microscopy during three key stages: invasion, development and egress. The detection of dynamin during invasion indicates the occurrence of endocytosis, while T. gondii egress appeared to be independent of dynamin participation. However, the presence of dynamin during T. gondii development suggests that this molecule plays undescribed roles in the tachyzoite's cell cycle.
Instituto de Biofí-sica, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ 21941-902, Brazil lucio@biof.ufrj.br.
Lucio Ayres
LA
Caldas
Instituto Nacional de Metrologia Normalizacao e Qualidade Industrial, Duque de Caxias, RJ 25250-020, Brazil.|Wellcome Trust Centre for Molecular Parasitology, University of Glasgow, 120 University Place Glasgow, Glasgow G12 8QQ, UK.
Leandro Lemgruber
LL
Soares
Microbiologia, UEZO, Av. Manuel Caldeira de Alvarenga, Rio de Janeiro, RJ 23070-200, Brazil.
Sergio
S
Henrique Seabra
Instituto de Biofí-sica, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ 21941-902, Brazil.
Marcia
M
Attias
Instituto de Biofí-sica, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ 21941-902, Brazil.
Wanderley
W
de Souza
D000199:Actins / Q000378:metabolism; D002453:Cell Cycle; D020285:Cryoelectron Microscopy; D034281:Dynamins / Q000378:metabolism*; D006790:Host-Parasite Interactions; D007150:Immunohistochemistry; D018613:Microscopy, Confocal; D014122:Toxoplasma / Q000472:pathogenicity / Q000502:physiology* / Q000648:ultrastructure; D014123:Toxoplasmosis / Q000378:metabolism* / Q000469:parasitology* / Q000473:pathology
D000199:Actins; D034281:Dynamins
Toxoplasma gondii; dynamin; host-pathogen interactions