A 30-InDel Assay for Genetic Variation and Population Structure Analysis of Chinese Tujia Group.
In the present study, thirty autosomal insertion and deletion polymorphic loci were simultaneously amplified and genotyped in a multiplex system, and their allelic frequencies as well as several forensic parameters were obtained in a sample of 236 unrelated healthy Tujia individuals. All the loci were in Hardy-Weinberg equilibrium after applying a Bonferroni correction and all pair-wise loci showed no significant linkage disequilibrium. These loci were observed to be relatively informative and discriminating, quite efficient for forensic applications. Allelic frequencies of 30 loci were compared between the Tujia group and other reference populations, and the results of analysis of molecular variance indicated the Tujia group showed the least significant differences with the Shanghai Han at one locus, and the most with Central Spanish population at 22 loci. We analyzed the population genetic structure by the principal component analysis, the clustering of STRUCTURE program and a Neighbor-Joining tree, and then evaluated the genetic relationships among Tujia and other 15 populations.
Blood Center of Shaanxi Province, Xi'an, Shaanxi 710061, P. R. China.|Institute of Brain and Behavioral Sciences, College of Life Sciences, Shaanxi Normal University, Xi'an, Shaanxi 710062, P. R. China.
Chunmei
C
Shen
Department of Forensic Genetics, School of Forensic Medicine, Southern Medical University, Guangzhou 510515, P. R. China.|Key Laboratory of Shaanxi Province for Craniofacial Precision Medicine Research, College of Stomatology, Xi'an Jiaotong University, Xi'an, Shaanxi 710004, P. R. China.|Clinical Research Center of Shaanxi Province for Dental and Maxillofacial Diseases, College of Stomatology, Xi'an Jiaotong University, Xi'an, Shaanxi 710004, P. R. China.
Bofeng
B
Zhu
Department of General Dentistry, Stomatological Hospital, Xi'an Jiaotong University, Xi'an, Shaanxi 710004, P. R. China.
Tianhua
T
Yao
Department of Endodontics, Stomatological Hospital, Xi'an Jiaotong University, Xi'an, Shaanxi 710004, P. R. China.
Zhidan
Z
Li
Institute of Forensic Science of Anhui Public Security Department, Hefei, Anhui 230061, P. R. China.
Yudang
Y
Zhang
Key Laboratory of Genome Sciences, Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing 100101, P. R. China.
Jiangwei
J
Yan
College of Biological Technology, Xi'an University, Xi'an, Shaanxi 710065, P. R. China.
Bo
B
Wang
Department of Neurosurgery, Hong-hui Hospital, Xi'an Jiaotong University College of Medicine, Xi'an, Shaanxi 710054, P. R. China.
Xiaohua
X
Bie
Institute of Brain and Behavioral Sciences, College of Life Sciences, Shaanxi Normal University, Xi'an, Shaanxi 710062, P. R. China.
Fadao
F
Tai
D044466:Asian People / Q000235:genetics*; D002681:China; D005787:Gene Frequency; D060005:Genotyping Techniques; D006801:Humans; D054643:INDEL Mutation; D015810:Linkage Disequilibrium; D018895:Microsatellite Repeats; D010802:Phylogeny; D011110:Polymorphism, Genetic; D044465:White People / Q000235:genetics