CbRCI35, a Cold Responsive Peroxidase fromCapsella bursa-pastorisRegulates Reactive Oxygen Species Homeostasis and Enhances Cold Tolerance in Tobacco.
Low temperature affects gene regulatory networks and alters cellular metabolism to inhibit plant growth. Peroxidases are widely distributed in plants and play a large role in adjusting and controlling reactive oxygen species (ROS) homeostasis in response to abiotic stresses such as low temperature. The Rare Cold-Inducible 35 gene fromCapsella bursa-pastoris(CbRCI35) belongs to the type III peroxidase family and has been reported to be a cold responsive gene in plants. Here we performed an expressional characterization ofCbRCI35under cold and ionic liquid treatments. The promoter ofCbRCI35was also cloned and its activity was examined using the GUS reporter system. CbRCI35 protein was localized in the cytoplasm according to sequence prediction and GFP fusion assay. Heterologous expression tests revealed thatCbRCI35conferred enhanced resistance to low temperature and activated endogenous cold responsive signaling in tobacco. Furthermore, in the normal condition the ROS accumulation was moderately enhanced while after chilling exposure superoxide dismutase activity was increased inCbRCI53transgenic plants. The ROS metabolism related genes expression was altered accordingly. We conclude thatCbRCI35modulates ROS homeostasis and contributes to cold tolerance in plants.
State Key Laboratory of Genetic Engineering, Institute of Plant Biology, School of Life Sciences, Fudan University Shanghai, China.
Mingqi
M
Zhou
State Key Laboratory of Genetic Engineering, Institute of Plant Biology, School of Life Sciences, Fudan University Shanghai, China.
Weiwei
W
Li
State Key Laboratory of Genetic Engineering, Institute of Plant Biology, School of Life Sciences, Fudan University Shanghai, China.
Ye
Y
Zheng
Research Institute of Subtropical Forestry, Chinese Academy of Forestry Fuyang, China.
Ping
P
Lin
Research Institute of Subtropical Forestry, Chinese Academy of Forestry Fuyang, China.
Xiaohua
X
Yao
State Key Laboratory of Genetic Engineering, Institute of Plant Biology, School of Life Sciences, Fudan University Shanghai, China.
Juan
J
Lin
CbRCI35; ROS; cold tolerance; gene expression; tobacco