Sensitive detection of estradiol based on ligand binding domain of estrogen receptor and gold nanoparticles.
With increasing concerns of estrogenic effects of endocrine disrupting compounds, the development of simple detection assay for these compounds is an ongoing need. Herein, a simple, rapid, and highly sensitive assay for estradiol (E2) detection was developed using the ligand binding domain of estrogen receptor α (LBD-ERα), the receptor interacting domain of steroid receptor co-activator 1 (RID-SRC1), and gold nanoparticles (AuNPs). The colloidal AuNPs could be stabilized against a salt-induced aggregation by adding LBD-ERα protein. However, with the presence of E2, the specific binding of LBD-ERα protein and E2 led to a salt-induced aggregation of AuNPs as seeing from a color change from red to blue. This developed assay exhibited a high sensitivity for E2 detection with the limit of detection (LOD) of 2.62 × 10-14M. When the RID-SRC1 protein was included, the detection sensitivity was increased, which the LOD for E2 was at 1.20 × 10-15M. This assay was specific for a detection of E2 but not progesterone, the negative control ligand. Results of this work clearly showed the efficiency of developed assay for E2 detection, which possibly further developed for an onsite monitoring of E2.
Department of Biochemistry, Faculty of Science, Khon Kaen University, Khon Kaen 40002, Thailand.
Pimchanok
P
Busayapongchai
School of Biology, Institute of Science, Suranaree University of Technology, Nakhon Ratchasima 30000, Thailand. Electronic address: ssinee@sut.ac.th.
Sineenat
S
Siri
D004958:Estradiol / Q000032:analysis*; D047628:Estrogen Receptor alpha / Q000737:chemistry*; D005260:Female; D006046:Gold / Q000737:chemistry*; D006801:Humans; D008024:Ligands; D061986:MCF-7 Cells; D053768:Metal Nanoparticles / Q000737:chemistry*; D000072417:Protein Domains
D047628:Estrogen Receptor alpha; D008024:Ligands; D004958:Estradiol; D006046:Gold
Co-activator; Colorimetric detection; Estradiol; Estrogen receptor; Gold nanoparticles