Bacterial Binding, Phagocytosis, and Killing: Measurements Using Colony Forming Units.
Herein we provide a colony forming unit (CFU)-based counting method for quantitating the bacterial binding, phagocytosis, and killing capacity of phagocytes. Although these functions can be measured by immunofluorescence and dye-based assays, quantitating CFUs is comparatively inexpensive and easy to perform. The protocol described below is easily modified for use with different phagocytes (e.g., macrophages, neutrophils, cell lines), types of bacteria or opsonic conditions.
Department of Pathology and Molecular Medicine, McMaster University, Hamilton, ON, Canada, L8N 3Z5.|McMaster Immunology Research Centre, McMaster University, 1280 Main Street West, Hamilton, ON, Canada, L8S 4K1.|M. G. DeGroote Institute for Infectious Disease Research, 1280 Main Street West, Hamilton, ON, Canada, L8S 4K1.
Kyle E
KE
Novakowski
Department of Pathology and Molecular Medicine, McMaster University, Hamilton, ON, Canada, L8N 3Z5.|McMaster Immunology Research Centre, McMaster University, 1280 Main Street West, Hamilton, ON, Canada, L8S 4K1.|M. G. DeGroote Institute for Infectious Disease Research, 1280 Main Street West, Hamilton, ON, Canada, L8S 4K1.
Dessi
D
Loukov
Department of Pathology and Molecular Medicine, McMaster University, Hamilton, ON, Canada, L8N 3Z5.|McMaster Immunology Research Centre, McMaster University, 1280 Main Street West, Hamilton, ON, Canada, L8S 4K1.|M. G. DeGroote Institute for Infectious Disease Research, 1280 Main Street West, Hamilton, ON, Canada, L8S 4K1.
Vikash
V
Chawla
Department of Pathology and Molecular Medicine, McMaster University, Hamilton, ON, Canada, L8N 3Z5. bowdish@mcmaster.ca.|McMaster Immunology Research Centre, McMaster University, 1280 Main Street West, Hamilton, ON, Canada, L8S 4K1. bowdish@mcmaster.ca.|M. G. DeGroote Institute for Infectious Disease Research, 1280 Main Street West, Hamilton, ON, Canada, L8S 4K1. bowdish@mcmaster.ca.
Dawn M E
DM
Bowdish
D000818:Animals; D001419:Bacteria / Q000187:drug effects / Q000378:metabolism*; D003114:Colony-Forming Units Assay / Q000379:methods*; D005839:Gentamicins / Q000494:pharmacology; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism; D050296:Microbial Viability / Q000187:drug effects; D010587:Phagocytosis / Q000187:drug effects; D012756:Sheep
D005839:Gentamicins
Bacterial killing; Gentamicin protection; Macrophage; Particle binding; Phagocyte; Phagocytosis