Humanin ameliorates diazepam-induced memory deficit in mice.
Humanin (HN) is an endogenous 24-residue peptide. A highly potent HN derivative, S14G-HN, which has a substitution of serine 14 to glycine, reduced amyloid burden and suppressed cognitive impairment in a mouse model of Alzheimer's disease. S14G-HN also suppressed amnesia induced by a muscarinic receptor antagonist in rodents. To understand the effects of HN on brain function, we tested the effect of S14G-HN on diazepam (DZP)-induced memory impairment and anxiety in mice using the object recognition test and zero-maze test, respectively. Intraperitoneal injection of S14G-HN reversed the DZP-induced memory deficit, whereas no significant change was observed in behavioral markers of anxiety. S14G-HN had no effect on locomotor activity in either test, indicating that S14G-HN did not affect physical functioning or motivation. These results suggest that HN preferentially influences cognitive function but not emotional function in the central nervous system.
Department of Information and Communication Sciences, Faculty of Science and Technology, Sophia University, Japan.
Minetaka
M
Murakami
Department of Information and Communication Sciences, Faculty of Science and Technology, Sophia University, Japan.
Masatoshi
M
Nagahama
Department of Information and Communication Sciences, Faculty of Science and Technology, Sophia University, Japan.
Takuma
T
Maruyama
Department of Information and Communication Sciences, Faculty of Science and Technology, Sophia University, Japan. Electronic address: niikura@sophia.ac.jp.
Takako
T
Niikura
D000544:Alzheimer Disease / Q000188:drug therapy*; D000818:Animals; D000927:Anticonvulsants / Q000494:pharmacology; D003071:Cognition / Q000187:drug effects*; D003975:Diazepam / Q000494:pharmacology; D004195:Disease Models, Animal; D047908:Intracellular Signaling Peptides and Proteins / Q000494:pharmacology*; D008297:Male; D018782:Maze Learning / Q000187:drug effects; D008569:Memory Disorders / Q000188:drug therapy*; D051379:Mice; D018696:Neuroprotective Agents / Q000494:pharmacology*
D000927:Anticonvulsants; D047908:Intracellular Signaling Peptides and Proteins; D018696:Neuroprotective Agents; C427211:humanin; D003975:Diazepam
Amnesia; Diazepam; GABAergic; Humanin