Comparison of cytotoxicity and genotoxicity effects of silver nanoparticles on human cervix and breast cancer cell lines.
The wide application of silver nanoparticles (AgNPs) has pointed out the need to evaluate their potential risk and toxic effects on human health. Herein, the cytotoxic effects of Argovit™ AgNPs were evaluated on eight cancer cell lines. Further cytotoxic studies were performed in gynecological cancer cell lines from cervical (HeLa) and breast (MDA-MB-231 and MCF7) cancer. In both cases, the half maximal inhibitory concentration (IC50) of AgNPs produced the formation of reactive oxygen species (ROS) after 24 h of incubation, but it was not statistically significant compared with untreated cells. However, HeLa, MDA-MB-231, and MCF7 cells treated with the maximal IC of AgNPs induced the formation of ROS either at 12 or 24 h of incubation. Genotoxicity achieved by comet assay in HeLa, MDA-MB-231, and MCF7 cells revealed that exposure to IC50of AgNPs does not induced noticeable DNA damage in the cells. However, the IC of AgNPs provoked severe DNA damage after 12 and 24 h of exposure. We conclude that, Argovit (polyvinylpyrrolidone-coated AgNPs) induce a cytotoxic effect in a time and dose-dependent manner in all the eight cancer cell lines tested. Nevertheless, the genotoxic effect is mainly restricted by the concentration effect. The results contribute to explore new therapeutic applications of AgNPs for malignances in murine models and to study in deep the cytotoxic and genotoxic effects of AgNPs in healthy cells at the surrounding tissue of the neoplasia.
1 Center of Nanosciences and Nanotechnology, National Autonomous University of Mexico. Ensenada, Baja California, Mexico.|2 Catedras CONACYT Fellow at Center of Nanosciences and Nanotechnology, National Autonomous University of Mexico.
K
K
Juarez-Moreno
1 Center of Nanosciences and Nanotechnology, National Autonomous University of Mexico. Ensenada, Baja California, Mexico.
E B
EB
Gonzalez
3 Master and Doctoral Program in Science and Engineering at FIAD, Autonomous University of Baja California, Mexico.
N
N
Girón-Vazquez
4 School of Chemistry, Autonomous University of Baja California, Mexico.
R A
RA
Chávez-Santoscoy
1 Center of Nanosciences and Nanotechnology, National Autonomous University of Mexico. Ensenada, Baja California, Mexico.|2 Catedras CONACYT Fellow at Center of Nanosciences and Nanotechnology, National Autonomous University of Mexico.
J D
JD
Mota-Morales
5 Ensenada Center for Science and Research and Higher Education, Ensenada, Baja California, Mexico.
L L
LL
Perez-Mozqueda
1 Center of Nanosciences and Nanotechnology, National Autonomous University of Mexico. Ensenada, Baja California, Mexico.|2 Catedras CONACYT Fellow at Center of Nanosciences and Nanotechnology, National Autonomous University of Mexico.
M R
MR
Garcia-Garcia
6 Tomsk Polytechnic University, Tomsk Oblast, Russia.
A
A
Pestryakov
1 Center of Nanosciences and Nanotechnology, National Autonomous University of Mexico. Ensenada, Baja California, Mexico.
N
N
Bogdanchikova
D017209:Apoptosis / Q000187:drug effects; D001943:Breast Neoplasms; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D020552:Comet Assay; D004249:DNA Damage; D004305:Dose-Response Relationship, Drug; D005260:Female; D006801:Humans; D053768:Metal Nanoparticles / Q000633:toxicity*; D017382:Reactive Oxygen Species / Q000378:metabolism; D012834:Silver / Q000633:toxicity*; D002583:Uterine Cervical Neoplasms
D017382:Reactive Oxygen Species; D012834:Silver
Silver nanoparticles; cancer; cytotoxicity; genotoxicity; nanobiotechnology; nanotoxicology; triple-negative breast cancer