Antigenotoxic Effect of Piperine in Broiler Chickens Intoxicated with Aflatoxin B1.
Piperine is an abundant amide extracted from black pepper seeds which has been shown to have protective effects against cytotoxic and genotoxic carcinogenesis induced by certain chemical carcinogens and aflatoxin B₁ (AFB₁) in vitro. The aim of this work was to study, in vivo, the antigenotoxic potential of feed-added piperine on broiler chickens experimentally intoxicated with AFB₁, using micronucleus and comet assays. The antigenotoxicity assessment of 9-day-old chicks was performed on a total of 60 chickens divided into four groups of 15 broilers each: (C) control, (P) 60 mg·piperine kg-1feed, (A) 0.5 mg·AFB₁·kg-1body weight, (daily by oral route), and (P + A) co-treatment with piperine and AFB₁. The experiment was conducted for 26 days. Chicks intoxicated with AFB₁ showed significant genotoxic effects in the first 24 h post intoxication, and the effects remained in the other periods analyzed (48, 72, and 96 h and 26 days of treatment). The DNA damage in peripheral blood cells, the number of erythrocytes with micronuclei, and polychromatic-to-normochromatic erythrocyte ratio were significantly reduced or absent in the piperine/AFB₁ group. No significant differences were observed between the group piperine/AFB₁ and the control and piperine-alone groups. The addition 60 mg·kg-1of piperine to the diet of the broiler chicks was safe, promoting beneficial effects in poultry health with respect to the toxic effects 0.5 mg·AFB₁·kg-1body weight.
BIOINOVAR-Biotecnologia, Unidade de Biocatálise, Bioprodutos e Bioenergia, Instituto de Microbiologia Paulo de Góes, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ 21941-901, Brazil. verocardoso@micro.ufrj.br.
Verônica
V
da Silva Cardoso
BIOINOVAR-Biotecnologia, Unidade de Biocatálise, Bioprodutos e Bioenergia, Instituto de Microbiologia Paulo de Góes, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ 21941-901, Brazil. abvermelho@micro.ufrj.br.
Alane Beatriz
AB
Vermelho
Instituto de Zootecnia, Universidade Federal Rural do Rio de Janeiro, Seropédica, RJ 23890-000, Brazil. criblima@terra.com.br.
Cristina Amorim
CA
Ribeiro de Lima
Instituto de Veterinária, Universidade Federal Rural do Rio de Janeiro, Seropédica, RJ 23890-000, Brazil. jessicamop@hotmail.com.
Jéssica
J
Mendes de Oliveira
Instituto de Ciências Exatas, Universidade Federal Rural do Rio de Janeiro, Seropédica, RJ 23890-000, Brazil. marco@ufrrj.br.
Marco Edilson
ME
Freire de Lima
Instituto de Veterinária, Universidade Federal Rural do Rio de Janeiro, Seropédica, RJ 23890-000, Brazil. lpinto_silva@yahoo.com.
Lúcia Helena
LH
Pinto da Silva
Instituto de Veterinária, Universidade Federal Rural do Rio de Janeiro, Seropédica, RJ 23890-000, Brazil. gdireito@ufrrj.br.
Glória Maria
GM
Direito
Instituto de Veterinária, Universidade Federal Rural do Rio de Janeiro, Seropédica, RJ 23890-000, Brazil. danelli@ufrrj.br.
Maria das Graças
MD
Miranda Danelli
D016604:Aflatoxin B1 / Q000633:toxicity*; D000470:Alkaloids / Q000494:pharmacology*; D000821:Animal Feed; D000818:Animals; D016588:Anticarcinogenic Agents / Q000494:pharmacology*; D052117:Benzodioxoles / Q000494:pharmacology*; D002273:Carcinogens / Q000633:toxicity*; D002645:Chickens; D020552:Comet Assay; D004249:DNA Damage; D004032:Diet / Q000662:veterinary; D004435:Eating / Q000187:drug effects; D008297:Male; D015162:Micronucleus Tests; D010880:Piperidines / Q000494:pharmacology*; D053284:Polyunsaturated Alkamides / Q000494:pharmacology*; D015430:Weight Gain / Q000187:drug effects
D000470:Alkaloids; D016588:Anticarcinogenic Agents; D052117:Benzodioxoles; D002273:Carcinogens; D010880:Piperidines; D053284:Polyunsaturated Alkamides; D016604:Aflatoxin B1; C008922:piperine
Aspergillus; aflatoxin B1; broiler chicken; piperine