A microfluidic chip based model for the study of full thickness human intestinal tissue using dual flow.
The study of inflammatory bowel disease, including Ulcerative Colitis and Crohn's Disease, has relied largely upon the use of animal or cell culture models; neither of which can represent all aspects of the human pathophysiology. Presented herein is a dual flow microfluidic device which holds full thickness human intestinal tissue in a known orientation. The luminal and serosal sides are independently perfusedex vivowith nutrients with simultaneous waste removal for up to 72 h. The microfluidic device maintains the viability and integrity of the tissue as demonstrated through Haematoxylin & Eosin staining, immunohistochemistry and release of lactate dehydrogenase. In addition, the inflammatory state remains in the tissue after perfusion on the device as determined by measuring calprotectin levels. It is anticipated that this human model will be extremely useful for studying the biology and testing novel interventions in diseased tissue.
Faculty of Life Sciences, University of Hull , Cottingham Road, Hull HU6 7RX, United Kingdom.
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A
Dawson
Faculty of Life Sciences, University of Hull , Cottingham Road, Hull HU6 7RX, United Kingdom.
C
C
Dyer
Scarborough Hospital , Woodlands Drive, Scarborough Y012 6QL, United Kingdom.
J
J
Macfie
General Surgery, Castlehill Hospital , Castle Rd, Cottingham HU16 5JQ, United Kingdom.
J
J
Davies
Pathology Building, Hull Royal Infirmary , Anlaby Road, Hull HU3 2JZ, United Kingdom.
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L
Karsai
Faculty of Life Sciences, University of Hull , Cottingham Road, Hull HU6 7RX, United Kingdom.
J
J
Greenman
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M
Jacobsen