Desflurane impairs outcome of organotypic hippocampal slices in anin vitromodel of traumatic brain injury.
Decreased mortality and disability after traumatic brain injury is a significant medical challenge. Desflurane, a widely used volatile anesthetic has proven to be neuroprotective in a variety ofin vitroandin vivomodels of ischemic brain injury. The aim of this study was to investigate whether desflurane exhibits neuroprotective properties in anin vitromodel of traumatic brain injury. Organotypic hippocampal slice cultures were prepared from brains of 5-7-day-old C57/BL6 mouse pups. After 14 days of culture, the slices were subjected to a focal mechanical trauma and thereafter incubated with three different concentrations of desflurane (2, 4 and 6%) for 2, 24 and 72 hours. Cell injury was assessed with propodium iodide uptake. Our results showed that after 2 hours of desflurane exposure, no significant change in trauma intensity was observed. However, 2% and 4% desflurane could reduce the trauma intensity significantly in the no trauma group than in the no desflurane and trauma group. Incubation with 4% desflurane for 24 hours doubled the trauma intensity in comparison to the trauma control group and the trauma intensity further increased after 72 hours of incubation. Furthermore, a dose-dependent increase of trauma intensity after 24 hours exposure was observed. Our results suggest that a general neuroprotective attribute of desflurane in anin vitromodel of traumatic brain injury was not observed.
Department of Anesthesiology, RWTH Aachen University Hospital, Aachen, Germany.
Matthias
M
Krings
Department of Neurosurgery, RWTH Aachen University Hospital, Aachen, Germany.
Anke
A
Höllig
Department of Anesthesiology, RWTH Aachen University Hospital, Aachen, Germany.
Jingjin
J
Liu
Department of Anesthesiology, RWTH Aachen University Hospital, Aachen, Germany.
Linda
L
Grüsser
Department of Anesthesiology, RWTH Aachen University Hospital, Aachen, Germany.
Rolf
R
Rossaint
Department of Anesthesiology, RWTH Aachen University Hospital, Aachen, Germany.
Mark
0000-0002-7930-0270
M
Coburn
desflurane; neuroprotection; organotypic hippocampal slices; traumatic brain injury