Epithelial cell adhesion molecule independent capture of non-small lung carcinoma cells with peptide modified microfluidic chip.
Circulating tumor cells (CTCs) present in the blood of patients with non-hematological cancers are accessible sources for diagnosis and monitoring of cancers. By the aid of the ability of the anti-EpCAM antibody to recognize the epithelial cells, microsystem-based technologies provide robust means for effectively detecting CTCs in vitro. Considering the EpCAM expression is down-regulated during epithelial-mesenchymal transition (EMT) process, the amount of CTCs detected based on anti-EpCAM antibody is underestimated. In our study, the A549 cells targeting peptide (A-1 peptide), as the substitute of anti-EpCAM antibody, was introduced to microfluidic chip to capture A549 cells. Our results showed that both epithelial-like and mesenchymal-like A549 cells could efficiently be captured by the A-1 peptide modified microfluidic chip, and the capture efficiency for epithelial-like cells is comparable to that captured by the EpCAM antibody. Thus, we concluded that the peptide could be a better supplement to the EpCAM antibody for capturing CTCs in microfluidic system with broader spectrum.
Key Laboratory of Nano-Bio Interface, Division of Nanobiomedicine, Suzhou institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences, Suzhou 215123, China.
Kefeng
K
Pu
Key Laboratory of Nano-Bio Interface, Division of Nanobiomedicine, Suzhou institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences, Suzhou 215123, China; Suzhou Institute of Nano-Tech and Nano-Bionics, CAS, University of Chinese Academy of Sciences, Beijing 100049, China.
Chunlin
C
Li
Key Laboratory of Nano-Bio Interface, Division of Nanobiomedicine, Suzhou institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences, Suzhou 215123, China; Suzhou Institute of Nano-Tech and Nano-Bionics, CAS, University of Chinese Academy of Sciences, Beijing 100049, China.
Nengpan
N
Zhang
Key Lab of Nanodevices and Applications, Suzhou institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences, Suzhou 215123, China; School of Material Science and Engineering, Shanghai University, Shanghai 200444, China.
Hui
H
Wang
Key Lab of Nanodevices and Applications, Suzhou institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences, Suzhou 215123, China.
Wenjiang
W
Shen
Key Laboratory of Nano-Bio Interface, Division of Nanobiomedicine, Suzhou institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences, Suzhou 215123, China. Electronic address: ymzhu2008@sinano.ac.cn.
Yimin
Y
Zhu
D000072283:A549 Cells; D000951:Antigens, Neoplasm / Q000737:chemistry / Q000276:immunology; D014408:Biomarkers, Tumor / Q000097:blood; D015374:Biosensing Techniques; D002289:Carcinoma, Non-Small-Cell Lung / Q000097:blood* / Q000473:pathology; D000071858:Epithelial Cell Adhesion Molecule / Q000097:blood* / Q000302:isolation & purification; D058750:Epithelial-Mesenchymal Transition; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D009360:Neoplastic Cells, Circulating / Q000737:chemistry; D010455:Peptides / Q000737:chemistry* / Q000276:immunology
D000951:Antigens, Neoplasm; D014408:Biomarkers, Tumor; C499379:EPCAM protein, human; D000071858:Epithelial Cell Adhesion Molecule; D010455:Peptides
A-1 peptide; Circulating tumor cells; Epithelial-mesenchymal transition; Microfluidic chips