Tissue engineering for neurodegenerative diseases using human amniotic membrane and umbilical cord.
Regenerative medicine, based on the use of stem cells, scaffolds and growth factors, has the potential to be a good approach for restoring damaged tissues of the central nervous system. This study investigated the use of human amniotic mesenchymal stem cells (hAMSC), human amniotic epithelial stem cells (hAESC), and human Wharton's jelly mesenchymal stem cells (hWJMSC) derived from human umbilical cord as a source of stem cells, and the potential of the human amniotic membrane (HAM) as a scaffold and/or source of growth factors to promote nerve regeneration. The hAMSC and hAESC obtained from HAM and the hWJMSC from umbilical cords were cultured in induction medium to obtain neural-like cells. The morphological differentiation of hAMSC, hAESC and hWJMSC into neural-like cells was evident after 4-5 days, when they acquired an elongated and multipolar shape, and at 21 days, when they expressed neural and glial markers. On other way, the HAM was completely decellularized without affecting the components of the basement membrane or the matrix. Subsequently, hAMSC, hAESC and hWJMSC differentiated into neural-like cells were seeded onto the decellularized HAM, maintaining their morphology. Finally, conditioned media from the HAM allowed proliferation of hAMSC, hAESC and hWJMSC differentiated to neural-like cells. Both HAM and umbilical cord are biomaterials with great potential for use in regenerative medicine for the treatment of neurodegenerative diseases.
Unidad de Criobiología-Banco de Tejidos, Complexo Hospitalario Universitario de A Coruña (CHUAC)-Instituto de Investigaciones Biomédicas A Coruña (INIBIC), As Xubias, s/n, 15006, A Coruña, Spain.
Anahí
A
Sanluis-Verdes
Unidad de Criobiología-Banco de Tejidos, Complexo Hospitalario Universitario de A Coruña (CHUAC)-Instituto de Investigaciones Biomédicas A Coruña (INIBIC), As Xubias, s/n, 15006, A Coruña, Spain.|Servicio de Cirugía General y del Aparato Digestivo, Complexo Hospitalario Universitario de A Coruña (CHUAC)-Instituto de Investigaciones Biomédicas A Coruña (INIBIC), As Xubias, s/n, 15006, A Coruña, Spain.
Namibia
N
Sanluis-Verdes
Grupo NEUROVER, Departamento de Biología Celular y Molecular, Facultad de Ciencias, Universidad de A Coruña (UDC), Campus de A Coruña, 15071, A Coruña, Spain.
María Jesús
MJ
Manso-Revilla
Grupo NEUROVER, Departamento de Biología Celular y Molecular, Facultad de Ciencias, Universidad de A Coruña (UDC), Campus de A Coruña, 15071, A Coruña, Spain.
Antonio Manuel
AM
Castro-Castro
Servicio de Anatomía Patológica, Complexo Hospitalario Universitario de A Coruña (CHUAC)-Instituto de Investigaciones Biomédicas A Coruña (INIBIC), As Xubias, s/n, 15006, A Coruña, Spain.
Jorge
J
Pombo-Otero
Biobanco A Coruña, Complexo Hospitalario Universitario de A Coruña (CHUAC)-Instituto de Investigaciones Biomédicas A Coruña (INIBIC), As Xubias, s/n, 15006, A Coruña, Spain.
María
M
Fraga-Mariño
Unidad de Criobiología-Banco de Tejidos, Complexo Hospitalario Universitario de A Coruña (CHUAC)-Instituto de Investigaciones Biomédicas A Coruña (INIBIC), As Xubias, s/n, 15006, A Coruña, Spain.
Jacinto
J
Sanchez-Ibañez
Biobanco A Coruña, Complexo Hospitalario Universitario de A Coruña (CHUAC)-Instituto de Investigaciones Biomédicas A Coruña (INIBIC), As Xubias, s/n, 15006, A Coruña, Spain. Nieves.Domenech.Garcia@sergas.es.
Nieves
N
Doménech
Unidad de Criobiología-Banco de Tejidos, Complexo Hospitalario Universitario de A Coruña (CHUAC)-Instituto de Investigaciones Biomédicas A Coruña (INIBIC), As Xubias, s/n, 15006, A Coruña, Spain. Esther.Rendal.Vazquez@sergas.es.
María Esther
ME
Rendal-Vázquez
D000650:Amnion / Q000737:chemistry / Q000166:cytology*; D049109:Cell Proliferation; D002478:Cells, Cultured; D004847:Epithelial Cells / Q000166:cytology*; D005260:Female; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D009416:Nerve Regeneration; D019636:Neurodegenerative Diseases / Q000628:therapy; D055495:Neurogenesis; D009474:Neurons / Q000166:cytology; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry; D014470:Umbilical Cord / Q000166:cytology*; D059631:Wharton Jelly / Q000166:cytology
Growth factors; Human amniotic membrane; Human umbilical cord; Neural differentiation; Scaffold; Tissue engineering