Evaluation of efficacy of probiotic (BIFILAC) onPorphyromonas gingivalis:In vitrostudy.
BACKGROUND
Periodontitis is inflammation of the periodontium and causes destruction of the connective tissue attachment of the teeth and alveolar bone.Porphyromonas gingivalisis the primary pathogen for the destructive periodontal diseases. The aim of the study is to evaluate the efficacy of probiotic onP. gingivalis.


METHODS
Anin vitrostudy was done to analyze the effectiveness of probiotic BIFILAC onP. gingivaliswas determined using disc diffusion method. The minimum inhibitory concentration for BIFILAC lozenges was also determined using microdilution method.


RESULTS
In disc diffusion method, the antibacterial activity of BIFILAC was analyzed using various concentrations such as 2.5, 5, 10, and 20 μg/ml, of which 20 μg/ml was proved to have a maximum inhibitory zone of 22 mm. In microdilution method, concentration ranging from 7.25 to 100 μg/ml was used and 25 μg/ml was found to have the minimum inhibitory effect onP. gingivalis.


CONCLUSIONS
The presentin vitrostudy confirms that probiotic BIFILAC has an antimicrobial effect againstP. gingivalis. Thus, proving that BIFILAC probiotic can be used as an adjunctive therapeutic modality in periodontitis.
Department of Periodontics, J.K.K. Nataraja Dental College and Hospital, Komarapalayam, Namakkal, Tamil Nadu, India.
Sugumari
S
Elavarasu
Department of Periodontics, J.K.K. Nataraja Dental College and Hospital, Komarapalayam, Namakkal, Tamil Nadu, India.
Thangakumaran
T
Suthanthiran
Department of Periodontics, J.K.K. Nataraja Dental College and Hospital, Komarapalayam, Namakkal, Tamil Nadu, India.
Arthiie
A
Thangavelu
Department of Periodontics, J.K.K. Nataraja Dental College and Hospital, Komarapalayam, Namakkal, Tamil Nadu, India.
Shiva Shangkharii
SS
Kanagaraj
Department of Periodontics, J.K.K. Nataraja Dental College and Hospital, Komarapalayam, Namakkal, Tamil Nadu, India.
Lakshmi
L
Mohandas
Department of Periodontics, J.K.K. Nataraja Dental College and Hospital, Komarapalayam, Namakkal, Tamil Nadu, India.
Santhosh
S
Sekar
Bacterial replacement therapy; Porphyromonas gingivalis; periodontitis; probiotics