Identification of Novel Sequence Types among Staphylococcus haemolyticus Isolated from Variety of Infections in India.
The aim of this study was to determine sequence types of 34 S. haemolyticus strains isolated from a variety of infections between 2013 and 2016 in India by MLST. The MEGA5.2 software was used to align and compare the nucleotide sequences. The advanced cluster analysis was performed to define the clonal complexes. MLST analysis showed 24 new sequence types (ST) among S. haemolyticus isolates, irrespective of sources and place of isolation. The finding of this study allowed to set up an MLST database on the PubMLST.org website using BIGSdb software and made available at http://pubmlst.org/shaemolyticus/. The data of this study thus suggest that MLST can be used to study population structure and diversity among S. haemolyticus isolates.
Infectious Disease Biology, Institute of Life Sciences, Nalco Square, Bhubaneswar-751023, India.
Sasmita
S
Panda
Infectious Disease Biology, Institute of Life Sciences, Nalco Square, Bhubaneswar-751023, India.
Smrutiti
S
Jena
Jhaveri Microbiology Centre, LV Prasad Eye Institute, Kallam Anji Reddy Campus, LV Prasad Marg, Banjara Hills, Hyderabad-500034, India.
Savitri
S
Sharma
Department of Microbiology, All India Institute of Medical Sciences, Ansari Nagar, New Delhi-110029, India.
Benu
B
Dhawan
Department of Microbiology, Institute of Medical Sciences, Banaras Hindu University, Varanasi-221005, India.
Gopal
G
Nath
Infectious Disease Biology, Institute of Life Sciences, Nalco Square, Bhubaneswar-751023, India.
Durg Vijai
http://orcid.org/0000-0002-9258-073X
DV
Singh
D015373:Bacterial Typing Techniques / Q000379:methods; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000235:genetics; D014644:Genetic Variation / Q000235:genetics; D005838:Genotype; D006801:Humans; D007194:India; D058885:Multilocus Sequence Typing / Q000379:methods; D010802:Phylogeny; D017422:Sequence Analysis, DNA / Q000379:methods; D013203:Staphylococcal Infections / Q000382:microbiology*; D041162:Staphylococcus haemolyticus / Q000235:genetics*
D004269:DNA, Bacterial