Triple-Internal Standard Based Glycan Structural Assignment Method for Capillary Electrophoresis Analysis of Carbohydrates.
Despite the ever growing use of capillary electrophoresis in biomedical research and the biopharmaceutical industry, the development of data interpretation methods is lagging behind. In this paper we report the design and implementation of a coinjected triple-internal standard method to alleviate the need of an accompanying run of the maltooligosaccharide ladder for glucose unit (GU) calculation. Based on the migration times of the coinjected standards of maltose, maltotriose, and maltopentadecaose (bracketing the peaks of interest), a data processing approach was designed and developed to set up a virtual ladder that was used for GU calculation. The data processing was tested in terms of the calculated GU values of human IgG glycans, and the resulting relative standard deviation was ≤1.07%. This approach readily supports high-throughput capillary electrophoresis systems by significantly speeding up the processing time for glycan structural assignment.
Horváth Csaba Memorial Institute for Bioanalytical Research, University of Debrecen , Debrecen, Hungary.|MTA-PE Translational Glycomics Group, University of Pannonia , Veszprem, Hungary.
Gabor
G
Jarvas
Horváth Csaba Memorial Institute for Bioanalytical Research, University of Debrecen , Debrecen, Hungary.|MTA-PE Translational Glycomics Group, University of Pannonia , Veszprem, Hungary.
Marton
M
Szigeti
SCIEX, Brea, California 92821, United States.
Jeff
J
Chapman
Horváth Csaba Memorial Institute for Bioanalytical Research, University of Debrecen , Debrecen, Hungary.|SCIEX, Brea, California 92821, United States.
Andras
A
Guttman
D002236:Carbohydrate Conformation; D019075:Electrophoresis, Capillary; D057166:High-Throughput Screening Assays; D006801:Humans; D007074:Immunoglobulin G / Q000737:chemistry*; D011134:Polysaccharides / Q000032:analysis*
D007074:Immunoglobulin G; D011134:Polysaccharides