Gene Profiles in a Smoke-Induced COPD Mouse Lung Model Following Treatment with Mesenchymal Stem Cells.
Mesenchymal stem cells (MSCs) effectively reduce airway inflammation and regenerate the alveolus in cigarette- and elastase-induced chronic obstructive pulmonary disease (COPD) animal models. The effects of stem cells are thought to be paracrine and immune-modulatory because very few stem cells remain in the lung one day after their systemic injection, which has been demonstrated previously. In this report, we analyzed the gene expression profiles to compare mouse lungs with chronic exposure to cigarette smoke with non-exposed lungs. Gene expression profiling was also conducted in a mouse lung tissue with chronic exposure to cigarette smoke following the systemic injection of human cord blood-derived mesenchymal stem cells (hCB-MSCs). Globally, 834 genes were differentially expressed after systemic injection of hCB-MSCs. Seven and 21 genes, respectively, were up-and downregulated on days 1, 4, and 14 after HCB-MSC injection. TheHbbandHba,genes with oxygen transport and antioxidant functions, were increased on days 1 and 14. A serine protease inhibitor was also increased at a similar time point after injection of hCB-MSCs. Gene Ontology analysis indicated that the levels of genes related to immune responses, metabolic processes, and blood vessel development were altered, indicating host responses after hCB-MSC injection. These gene expression changes suggest that MSCs induce a regeneration mechanism against COPD induced by cigarette smoke. These analyses provide basic data for understanding the regeneration mechanisms promoted by hCB-MSCs in cigarette smoke-induced COPD.
University of Ulsan College of Medicine, Seoul 05505, Korea.|Asan Institute for Life Sciences, Seoul 05505, Korea.
You-Sun
YS
Kim
Department of Pulmonology, Gazi University, Ankara, Turkey.
Nurdan
N
Kokturk
Asan Institute for Life Sciences, Seoul 05505, Korea.
Ji-Young
JY
Kim
University of Ulsan College of Medicine, Seoul 05505, Korea.|Department of Pulmonary and Critical Care Medicine, Asan Medical Center, Seoul 05505, Korea.
Sei Won
SW
Lee
University of Ulsan College of Medicine, Seoul 05505, Korea.
Jaeyun
J
Lim
Biomedical Research Institute, MEDIPOST Co., Ltd., Seoul, Korea.
Soo Jin
SJ
Choi
Biomedical Research Institute, MEDIPOST Co., Ltd., Seoul, Korea.
Wonil
W
Oh
University of Ulsan College of Medicine, Seoul 05505, Korea.|Department of Pulmonary and Critical Care Medicine, Asan Medical Center, Seoul 05505, Korea.
Yeon-Mok
YM
Oh
D000818:Animals; D036101:Cord Blood Stem Cell Transplantation / Q000379:methods; D004195:Disease Models, Animal; D005260:Female; D006801:Humans; D045164:Mesenchymal Stem Cell Transplantation / Q000379:methods*; D059630:Mesenchymal Stem Cells / Q000166:cytology; D051379:Mice; D008810:Mice, Inbred C57BL; D029424:Pulmonary Disease, Chronic Obstructive / Q000209:etiology / Q000235:genetics* / Q000628:therapy; D012906:Smoke; D014026:Nicotiana; D059467:Transcriptome
D012906:Smoke
gene profile; human cord blood-derived mesenchymal stem cells; microarray; smoke-induced chronic obstructive pulmonary disease