Tapirira guianensis Aubl. Extracts Inhibit Proliferation and Migration of Oral Cancer Cells Lines.
Cancer of the head and neck is a group of upper aerodigestive tract neoplasms in which aggressive treatments may cause harmful side effects to the patient. In the last decade, investigations on natural compounds have been particularly successful in the field of anticancer drug research. Our aim is to evaluate the antitumor effect ofTapirira guianensisAubl. extracts on a panel of head and neck squamous cell carcinoma (HNSCC) cell lines. Analysis of secondary metabolites classes in fractions ofT. guianensiswas performed using Nuclear Magnetic Resonance (NMR). Mutagenicity effect was evaluated by Ames mutagenicity assay. The cytotoxic effect, and migration and invasion inhibition were measured. Additionally, the expression level of apoptosis-related molecules (PARP, Caspases 3, and Fas) and MMP-2 was detected using Western blot. Heterogeneous cytotoxicity response was observed for all fractions, which showed migration inhibition, reduced matrix degradation, and decreased cell invasion ability. Expression levels of MMP-2 decreased in all fractions, and particularly in the hexane fraction. Furthermore, overexpression of FAS and caspase-3, and increase of cleaved PARP indicates possible apoptosis extrinsic pathway activation. Antiproliferative activity ofT. guianensisextract in HNSCC cells lines suggests the possibility of developing an anticancer agent or an additive with synergic activities associated with conventional anticancer therapy.
Molecular Oncology Research Center, Barretos Cancer Hospital, Barretos 14784-400, Brazil. renatokjso@gmail.com.
Renato José
RJ
Silva-Oliveira
Laboratory of Experimental Pathology, Federal University of São João del Rei-CCO/UFSJ, Divinópolis 35501-296, Brazil. gabrielafrancine_lp@hotmail.com.
Gabriela Francine
GF
Lopes
Laboratory of Mutagenesis, Federal University of São João del Rei-CCO/UFSJ, Divinópolis 35501-296, Brazil. luizfcamargos@yahoo.com.br.
Luiz Fernando
LF
Camargos
Medical School, Federal University of Minas Gerais-UFMG, Belo Horizonte 31270-901, Brazil. anamacielr@gmail.com.
Ana Maciel
AM
Ribeiro
Laboratory of Mutagenesis, Federal University of São João del Rei-CCO/UFSJ, Divinópolis 35501-296, Brazil. fabiosantos@ufsj.edu.br.
Fábio Vieira Dos
FV
Santos
Special Academic Unit of Physics and Chemistry, Federal University of Goiás, Catalão 75704-020, Brazil. richeleps@ufg.br.
Richele Priscila
RP
Severino
Special Academic Unit of Physics and Chemistry, Federal University of Goiás, Catalão 75704-020, Brazil. vanessa.pasqualotto@gmail.com.
Vanessa Gisele Pasqualotto
VG
Severino
Special Academic Unit of Physics and Chemistry, Federal University of Goiás, Catalão 75704-020, Brazil. apterezan@hotmail.com.
Ana Paula
AP
Terezan
Laboratory of Tissue Processing, Federal University of São João del Rei-CCO/UFSJ, Divinópolis 35501-296, Brazil. ralphthome@gmail.com.
Ralph Gruppi
RG
Thomé
Laboratory of Tissue Processing, Federal University of São João del Rei-CCO/UFSJ, Divinópolis 35501-296, Brazil. hbsufsj@gmail.com.
Hélio Batista Dos
HB
Santos
Molecular Oncology Research Center, Barretos Cancer Hospital, Barretos 14784-400, Brazil. ruireis.hcb@gmail.com.|Life and Health Sciences Research Institute (ICVS), Health Sciences School, University of Minho, Braga 4710-057, Portugal. ruireis.hcb@gmail.com.|3ICVS/3B's-PT Government Associate Laboratory, Braga 4710-057, Portugal. ruireis.hcb@gmail.com.
Rui Manuel
RM
Reis
Laboratory of Mutagenesis, Federal University of São João del Rei-CCO/UFSJ, Divinópolis 35501-296, Brazil. rosy@ufsj.edu.br.
Rosy Iara Maciel de Azambuja
RI
Ribeiro
D027926:Anacardiaceae / Q000737:chemistry*; D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects* / Q000235:genetics; D053148:Caspase 3 / Q000235:genetics / Q000378:metabolism; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects; D003094:Collagen / Q000737:chemistry; D004338:Drug Combinations; D004847:Epithelial Cells / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007797:Laminin / Q000737:chemistry; D020778:Matrix Metalloproteinase 2 / Q000235:genetics / Q000378:metabolism; D009152:Mutagenicity Tests; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D011065:Poly(ADP-ribose) Polymerases / Q000235:genetics / Q000378:metabolism; D011509:Proteoglycans / Q000737:chemistry; D012486:Salmonella typhimurium / Q000187:drug effects / Q000235:genetics; D019014:fas Receptor / Q000235:genetics / Q000378:metabolism
D000972:Antineoplastic Agents, Phytogenic; D004338:Drug Combinations; C491901:FAS protein, human; D007797:Laminin; D010936:Plant Extracts; D011509:Proteoglycans; D019014:fas Receptor; C059485:matrigel; D003094:Collagen; D011065:Poly(ADP-ribose) Polymerases; C505407:CASP3 protein, human; D053148:Caspase 3; C522361:MMP2 protein, human; D020778:Matrix Metalloproteinase 2
HNSCC; alkaloids; apoptosis markers; cytotoxic activity