Identification of High-risk Cryptic CRLF2 Rearrangements in B-Cell Acute Lymphoblastic Leukemia Utilizing an FGFR3/IGH Dual-Color Dual-Fusion DNA Probe Set.
B-cell acute lymphoblastic leukemia (B-ALL) is the most common childhood malignancy with gene rearrangements involving the IGH locus occurring in ∼5% of cases. Fluorescence in situ hybridization (FISH) probes targeting the IGH locus are not included in the standard children's oncology group (COG) fluorescence in situ hybridization panel. At our institute, we incorporated the use of FGFR3/IGH dual-color dual-fusion DNA probes for confirmation of aneuploidy 4 and 14 in diagnostic B-ALL specimens. Subsequently we have identified 4 B-ALL cases with cryptic CRLF2-IGH translocations that would otherwise have gone undetected. Detection of genetic alterations in B-ALL, such as CRLF2 rearrangements, may enhance patient risk stratification and therapy options in pediatric B-ALL.
*Department of Pathology, Medical College of Wisconsin †Wisconsin Diagnostic Laboratories ‡Department of Pathology §Division of Pediatric Oncology, Children's Hospital of Wisconsin, Milwaukee, WI.
Adam J
AJ
Robin
Jess F
JF
Peterson
John W
JW
Grignon
Gabriela
G
Gheorghe
Michael J
MJ
Burke
Peter
P
vanTuinen
D002648:Child; D002675:Child, Preschool; D015342:DNA Probes; D005260:Female; D015321:Gene Rearrangement; D006801:Humans; D007143:Immunoglobulin Heavy Chains / Q000235:genetics; D017404:In Situ Hybridization, Fluorescence; D007223:Infant; D008297:Male; D015452:Precursor B-Cell Lymphoblastic Leukemia-Lymphoma / Q000175:diagnosis* / Q000235:genetics; D051498:Receptor, Fibroblast Growth Factor, Type 3 / Q000235:genetics; D018121:Receptors, Cytokine / Q000235:genetics*; D012306:Risk
C498762:CRLF2 protein, human; D015342:DNA Probes; D007143:Immunoglobulin Heavy Chains; D018121:Receptors, Cytokine; C496365:FGFR3 protein, human; D051498:Receptor, Fibroblast Growth Factor, Type 3