Erythropoietin in the Locus coeruleus attenuates the ventilatory response to CO2in rats.
The Locus coeruleus (LC) is a pontine area that contributes to the CO2/pH chemosensitivity. LC cells express erythropoietin (Epo) receptors (EpoR), and Epo in the brainstem is a potent normoxic and hypoxic respiratory stimulant. However, a recent study showed that the intra-cisternal injection (ICI) of Epo antagonist does not alter the hypercapnic ventilatory response in mice. As ICI leads to a widespread dispersal of the product throughout the brainstem, in this work we evaluated the specific impact of Epo in the LC-mediated ventilatory response to CO2(by whole body plethysmography) in juvenile male Wistar rats. Normocapnic and hypercapnic ventilation were evaluated before and after unilateral microinjection of Epo (1ng/100nL) into the LC. To evaluate the long-term effect of Epo, the HcVR was re-evaluated 24h later. Our results show that Epo attenuates the hypercapnic ventilation. We conclude that Epo in the LC tunes the hypercapnia-induced hyperpnea.
Department of Animal Morphology and Physiology, Sao Paulo State University-FCAV, Jaboticabal, SP, Brazil.
Carlos A
CA
Silva
Department of Animal Morphology and Physiology, Sao Paulo State University-FCAV, Jaboticabal, SP, Brazil.
Mariane C
MC
Vicente
Centre de Recherche du CHU de Québec, Pavillon St. François d'Assise, Département de Pédiatrie, Faculté de Médecine, Université Laval, Québec, QC, Canada.
Luana
L
Tenorio-Lopes
Centre de Recherche du CHU de Québec, Pavillon St. François d'Assise, Département de Pédiatrie, Faculté de Médecine, Université Laval, Québec, QC, Canada. Electronic address: jorge.soliz@crchuq.ulaval.ca.
Jorge
J
Soliz
Department of Animal Morphology and Physiology, Sao Paulo State University-FCAV, Jaboticabal, SP, Brazil.
Luciane H
LH
Gargaglioni
D000704:Analysis of Variance; D000818:Animals; D002245:Carbon Dioxide / Q000494:pharmacology*; D004921:Erythropoietin / Q000494:pharmacology*; D007839:Functional Laterality; D006801:Humans; D006935:Hypercapnia / Q000139:chemically induced / Q000188:drug therapy*; D008125:Locus Coeruleus / Q000187:drug effects*; D008297:Male; D008845:Microinjections; D012123:Pulmonary Ventilation / Q000187:drug effects*; D051381:Rats; D017208:Rats, Wistar; D011994:Recombinant Proteins / Q000627:therapeutic use; D012119:Respiration / Q000187:drug effects*; D013990:Tidal Volume / Q000187:drug effects; D013997:Time Factors
C554270:EPO protein, human; D011994:Recombinant Proteins; D004921:Erythropoietin; D002245:Carbon Dioxide
Central chemosensitivity; Erythropoietin; Hypercapnia; Locus coeruleus; Ventilation