Knock-Down of a Tonoplast Localized Low-Affinity Nitrate TransporterOsNPF7.2Affects Rice Growth under High Nitrate Supply.
The large nitrate transporter 1/peptide transporter family (NPF) has been shown to transport diverse substrates, including nitrate, amino acids, peptides, phytohormones, and glucosinolates. However, the rice (Oryza sativa) root-specific family memberOsNPF7.2has not been functionally characterized. Here, our data show that OsNPF7.2 is a tonoplast localized low-affinity nitrate transporter, that affects rice growth under high nitrate supply. Expression analysis showed thatOsNPF7.2was mainly expressed in the elongation and maturation zones of roots, especially in the root sclerenchyma, cortex and stele. It was also induced by high concentrations of nitrate. Subcellular localization analysis showed that OsNPF7.2 was localized on the tonoplast of large and small vacuoles. Heterologous expression inXenopus laevisoocytes suggested that OsNPF7.2 was a low-affinity nitrate transporter. Knock-down ofOsNPF7.2retarded rice growth under high concentrations of nitrate. Therefore, we deduce thatOsNPF7.2plays a role in intracellular allocation of nitrate in roots, and thus influences rice growth under high nitrate supply.
Key Laboratory of South China Agricultural Plant Molecular Analysis and Genetic Improvement and Guangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of SciencesGuangzhou, China; University of Chinese Academy of SciencesBeijing, China.
Rui
R
Hu
Key Laboratory of South China Agricultural Plant Molecular Analysis and Genetic Improvement and Guangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of SciencesGuangzhou, China; University of Chinese Academy of SciencesBeijing, China.
Diyang
D
Qiu
Metabolic Biology Department, John Innes Centre Norwich, UK.
Yi
Y
Chen
Metabolic Biology Department, John Innes Centre Norwich, UK.
Anthony J
AJ
Miller
State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University Nanjing, China.
Xiaorong
X
Fan
Key Laboratory of South China Agricultural Plant Molecular Analysis and Genetic Improvement and Guangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences Guangzhou, China.
Xiaoping
X
Pan
Key Laboratory of South China Agricultural Plant Molecular Analysis and Genetic Improvement and Guangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences Guangzhou, China.
Mingyong
M
Zhang
Oryza sativa; OsNPF7.2; growth; nitrate; transporter