Molecularly imprinted electrochemical sensor based on bioinspired Au microflowers for ultra-trace cholesterol assay.
A novel imprinted sensor for ultra-trace cholesterol (CHO) detection based on electropolymerized aminothiophenol (ATP) molecularly imprinted polymer (MIP) on a glassy carbon electrode (GCE) modified with dopamine@graphene (DGr) and bioinspired Au microflowers has been developed in this work. As the specific recognition element, the bioinspired Au microflowers were formed by Au nanoparticles (AuNPs) and wrapped by bionic polydopamine film (PDA) through electropolymerization method. These excellent biocompatible materials could capture the target CHO effectively. The morphology of the MIP modified electrode was characterized by scanning electron microscopy (SEM) and atomic force microscope (AFM). The hydrogen-bonding interaction between templates and monomers was characterized by ultraviolet spectroscopy. Under the optimal experimental conditions, the sensor's linear response range was between 10-18and 10-13M, with a detection limit of 3.3×10-19M, which was much more sensitive than most available CHO detection methods in previously reports. Moreover, the MIP sensor exhibited high sensitivity for CHO, low interference, and good stability. The human serum samples analysis confirmed the applicability of this MIP sensor to quantitative analysis of ultra-trace CHO.
Department of Chemistry, Shanghai University, Shanghai 200444, PR China.
Hua
H
Yang
Department of Chemistry, Shanghai University, Shanghai 200444, PR China.
Li
L
Li
Department of Chemistry, Shanghai University, Shanghai 200444, PR China; Shanghai Key Laboratory of High Temperature Superconductors, Shanghai University, Shanghai 200444, PR China. Electronic address: wdingyp@sina.com.
Yaping
Y
Ding
Department of Chemistry and Molecular Biology, University of Gothenburg, Gothenburg S-41296, Sweden.
Daixin
D
Ye
Department of Chemistry, Shanghai University, Shanghai 200444, PR China.
Yingzi
Y
Wang
Department of Chemistry, Shanghai University, Shanghai 200444, PR China.
Shiqiang
S
Cui
Department of Chemistry, Shanghai University, Shanghai 200444, PR China.
Lanfeng
L
Liao
D000814:Aniline Compounds / Q000737:chemistry; D015374:Biosensing Techniques / Q000379:methods; D002784:Cholesterol / Q000097:blood*; D055664:Electrochemical Techniques / Q000379:methods*; D006046:Gold / Q000737:chemistry*; D006108:Graphite / Q000737:chemistry; D006801:Humans; D007211:Indoles / Q000737:chemistry*; D057230:Limit of Detection; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D054802:Molecular Imprinting / Q000379:methods*; D011108:Polymers / Q000737:chemistry*
D000814:Aniline Compounds; D007211:Indoles; D011108:Polymers; C568283:polydopamine; C005464:2-aminothiophenol; D006046:Gold; D006108:Graphite; D002784:Cholesterol
Au microflowers; Cholesterol; Molecularly imprinted electrochemical sensor; Polydopamine