The expression patterns of Tetratricopeptide repeat domain 36 (Ttc36).
Tetratricopeptide repeat domain 36 (Ttc36), whose coding protein belongs to tetratricopeptide repeat (TPR) motif family, has not been studied extensively. We for the first time showed that Ttc36 is evolutionarily conserved across mammals by bioinformatics. Rabbit anti-mouse Ttc36 polyclonal antibody was generated by injecting synthetic full-length peptides through "antigen intersection" strategy. Subsequently, we characterized Ttc36 expression profile in mouse, showing its expression in liver and kidney both from embryonic day 15.5 (E15.5) until adult, as well as in testis. Immunofluorescence staining showed that Ttc36 is diffusely expressed in liver, however, specifically in kidney cortex. Thus, we further compare Ttc36 with proximal tubules (PT) marker Lotus Tetragonolobus Lectin (LTL) and distal tubules (DT) marker Calbindin-D28k respectively by double immunofluorescence staining. Results showed the co-localization of Ttc36 with LTL rather than Calbindin-D28k. Collectively, on the basis of the expression pattern, Ttc36 is specifically expressed in proximal distal tubules.
Division of Molecular Nephrology and the Creative Training Center for Undergraduates, The Ministry of Education Key Laboratory of Laboratory Medical Diagnostics, The College of Laboratory Medicine, Chongqing Medical University, Chongqing, 400016, PR China; The Seventh Class of 2012 Year Entry, The Third Clinical College, Chongqing Medical University, Chongqing, 400016, PR China. Electronic address: zhouyuru93@163.com.
Yuru
Y
Zhou
Division of Molecular Nephrology and the Creative Training Center for Undergraduates, The Ministry of Education Key Laboratory of Laboratory Medical Diagnostics, The College of Laboratory Medicine, Chongqing Medical University, Chongqing, 400016, PR China. Electronic address: hqltsang@foxmail.com.
Qingling
Q
He
Department of Dermatology, West China Hospital, West China Medical School, Sichuan University, Chengdu, Sichuan, 610041, PR China. Electronic address: Cjh_111222@126.com.
Jihui
J
Chen
Division of Molecular Nephrology and the Creative Training Center for Undergraduates, The Ministry of Education Key Laboratory of Laboratory Medical Diagnostics, The College of Laboratory Medicine, Chongqing Medical University, Chongqing, 400016, PR China. Electronic address: liuyunhong2001@163.com.
Yunhong
Y
Liu
Division of Molecular Nephrology and the Creative Training Center for Undergraduates, The Ministry of Education Key Laboratory of Laboratory Medical Diagnostics, The College of Laboratory Medicine, Chongqing Medical University, Chongqing, 400016, PR China. Electronic address: zhaominmao@sina.com.
Zhaomin
Z
Mao
Division of Molecular Nephrology and the Creative Training Center for Undergraduates, The Ministry of Education Key Laboratory of Laboratory Medical Diagnostics, The College of Laboratory Medicine, Chongqing Medical University, Chongqing, 400016, PR China. Electronic address: zhongshilyu@sina.com.
Zhongshi
Z
Lyu
Division of Molecular Nephrology and the Creative Training Center for Undergraduates, The Ministry of Education Key Laboratory of Laboratory Medical Diagnostics, The College of Laboratory Medicine, Chongqing Medical University, Chongqing, 400016, PR China. Electronic address: dongshengni@outlook.com.
Dongsheng
D
Ni
Division of Molecular Nephrology and the Creative Training Center for Undergraduates, The Ministry of Education Key Laboratory of Laboratory Medical Diagnostics, The College of Laboratory Medicine, Chongqing Medical University, Chongqing, 400016, PR China. Electronic address: lys960110@sina.com.
Yaoshui
Y
Long
Division of Molecular Nephrology and the Creative Training Center for Undergraduates, The Ministry of Education Key Laboratory of Laboratory Medical Diagnostics, The College of Laboratory Medicine, Chongqing Medical University, Chongqing, 400016, PR China. Electronic address: 18883936591@163.com.
Pan
P
Ju
Division of Molecular Nephrology and the Creative Training Center for Undergraduates, The Ministry of Education Key Laboratory of Laboratory Medical Diagnostics, The College of Laboratory Medicine, Chongqing Medical University, Chongqing, 400016, PR China. Electronic address: keithljn@sina.cn.
Jianing
J
Liu
Division of Molecular Nephrology and the Creative Training Center for Undergraduates, The Ministry of Education Key Laboratory of Laboratory Medical Diagnostics, The College of Laboratory Medicine, Chongqing Medical University, Chongqing, 400016, PR China. Electronic address: guyupinglittle@outlook.com.
Yuping
Y
Gu
Division of Molecular Nephrology and the Creative Training Center for Undergraduates, The Ministry of Education Key Laboratory of Laboratory Medical Diagnostics, The College of Laboratory Medicine, Chongqing Medical University, Chongqing, 400016, PR China. Electronic address: zhouqin@cqmu.edu.cn.
Qin
Q
Zhou
D020816:Amino Acid Motifs / Q000235:genetics; D000818:Animals; D064092:Calbindin 1 / Q000096:biosynthesis / Q000235:genetics; D002352:Carrier Proteins / Q000096:biosynthesis / Q000235:genetics*; D018507:Gene Expression Regulation, Developmental / Q000235:genetics*; D007687:Kidney Tubules, Proximal / Q000378:metabolism; D037102:Lectins / Q000096:biosynthesis / Q000235:genetics; D008565:Membrane Proteins / Q000235:genetics*; D051379:Mice; D024101:Mitochondrial Proteins / Q000235:genetics*; D000072417:Protein Domains / Q000235:genetics*; D011817:Rabbits; D020449:Repetitive Sequences, Amino Acid / Q000235:genetics
D064092:Calbindin 1; D002352:Carrier Proteins; D037102:Lectins; D008565:Membrane Proteins; D024101:Mitochondrial Proteins; C000619574:Ttc36 protein, mouse; C027112:fucose-binding lectin
Proximal tubules; TPR protein; Ttc36