Tandem Affinity Purification Approach Coupled to Mass Spectrometry to Identify Post-translational Modifications of Histones Associated with Chromatin-Binding Proteins.
Protein purification by tandem affinity purification (TAP)-tag coupled to mass spectrometry analysis is usually used to reveal protein complex composition. Here we describe a TAP-tag purification of chromatin-bound proteins along with associated nucleosomes, which allow exhaustive identification of protein partners. Moreover, this method allows exhaustive identification of the post-translational modifications (PTMs) of the associated histones. Thus, in addition to partner characterization, this approach reveals the associated epigenetic landscape that can shed light on the function and properties of the studied chromatin-bound protein.
Centre National de la Recherche Scientifique CNRS-Université Paris Diderot, Sorbonne Paris Cité, Epigenetics and Cell Fate, UMR 7216 CNRS, Paris, France.
Sophie
S
Beyer
Centre National de la Recherche Scientifique CNRS-Université Paris Diderot, Sorbonne Paris Cité, Epigenetics and Cell Fate, UMR 7216 CNRS, Paris, France.
Philippe
P
Robin
Centre National de la Recherche Scientifique CNRS-Université Paris Diderot, Sorbonne Paris Cité, Epigenetics and Cell Fate, UMR 7216 CNRS, Paris, France. slimane.aitsiali@univ-paris-diderot.fr.
Slimane
S
Ait-Si-Ali
D002478:Cells, Cultured; D002843:Chromatin / Q000378:metabolism; D002846:Chromatography, Affinity; D006657:Histones / Q000302:isolation & purification* / Q000378:metabolism; D011499:Protein Processing, Post-Translational; D053719:Tandem Mass Spectrometry
D002843:Chromatin; D006657:Histones
Chromatin; Epigenetics; Histones; Mass spectrometry; Post-translational modifications; TAP-tag