Differential diagnosis of lung cancer, its metastasis and chronic obstructive pulmonary disease based on serum Vegf, Il-8 and MMP-9.
Chronic obstructive pulmonary disease (COPD) patients are at higher risk of developing lung cancer and its metastasis, but no suitable biomarker has been reported for differential diagnosis of these patients. Levels of serum biomarkers (VEGF, IL-8, MMP-9 and MMP-2) were analyzed in these patients, which were compared with healthy donors (HD). Levels of VEGF (P < 0.005) and MMP-9 (P < 0.05) were significantly higher in COPD patients than HD. Compared to HD, a decrease in IL-8 (~8.1 folds; P < 0.0001) but an increase in MMP-9 (~1.6 folds; P < 0.05) levels were observed in the lung cancer patients. Cancer patients showed significantly (P < 0.005) lower levels of serum VEGF (1.9 folds) and IL-8 (~9 folds) than the COPD patients. VEGF level was significantly higher (2.6 folds; P < 0.0005) in metastatic than non-metastatic cancer patients. However, MMP-2 didn't show significant variation in these patients. The Youden's index (YI) values for lung cancer diagnosis in HD using IL-8 was 0.55 with 83.3% overall accuracy. VEGF was able to diagnose COPD in HD with better YI (0.38) and overall accuracy (70.6%). IL-8 was able to diagnose cancer in COPD patients and HD with YI values of 0.35, 0.55 with 71% and 83.3% overall accuracy, respectively.
Radiation Biology and Health Sciences Division, Bhabha Atomic Research Centre, Trombay, Mumbai 400 085, India.
Murali M S
MM
Balla
Radiation Biology and Health Sciences Division, Bhabha Atomic Research Centre, Trombay, Mumbai 400 085, India.|Homi Bhabha National Institute, Anushakti Nagar, Mumbai 400 094, India.
Sejal
S
Desai
Tata Memorial Hospital, Dr E. Borges Road, Parel, Mumbai, 400012, India.
Pallavi
P
Purwar
Radiation Biology and Health Sciences Division, Bhabha Atomic Research Centre, Trombay, Mumbai 400 085, India.|Homi Bhabha National Institute, Anushakti Nagar, Mumbai 400 094, India.
Amit
A
Kumar
Medical Division, Bhabha Atomic Research Centre, Anushakti Nagar, Mumbai 400 094, India.
Prashant
P
Bhandarkar
Homi Bhabha National Institute, Anushakti Nagar, Mumbai 400 094, India.|Medical Division, Bhabha Atomic Research Centre, Anushakti Nagar, Mumbai 400 094, India.
Yogesh K
YK
Shejul
Homi Bhabha National Institute, Anushakti Nagar, Mumbai 400 094, India.|Tata Memorial Hospital, Dr E. Borges Road, Parel, Mumbai, 400012, India.
C S
CS
Pramesh
Homi Bhabha National Institute, Anushakti Nagar, Mumbai 400 094, India.|Tata Memorial Hospital, Dr E. Borges Road, Parel, Mumbai, 400012, India.
S
S
Laskar
Radiation Biology and Health Sciences Division, Bhabha Atomic Research Centre, Trombay, Mumbai 400 085, India.|Homi Bhabha National Institute, Anushakti Nagar, Mumbai 400 094, India.
Badri N
BN
Pandey
D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000097:blood; D016022:Case-Control Studies; D003937:Diagnosis, Differential; D005260:Female; D006801:Humans; D016209:Interleukin-8 / Q000097:blood*; D008175:Lung Neoplasms / Q000097:blood* / Q000175:diagnosis* / Q000556:secondary; D008297:Male; D020778:Matrix Metalloproteinase 2 / Q000097:blood; D020780:Matrix Metalloproteinase 9 / Q000097:blood*; D008875:Middle Aged; D029424:Pulmonary Disease, Chronic Obstructive / Q000097:blood* / Q000175:diagnosis*; D042461:Vascular Endothelial Growth Factor A / Q000097:blood*
D014408:Biomarkers, Tumor; C508606:CXCL8 protein, human; D016209:Interleukin-8; C467484:VEGFA protein, human; D042461:Vascular Endothelial Growth Factor A; C522361:MMP2 protein, human; D020778:Matrix Metalloproteinase 2; C579270:MMP9 protein, human; D020780:Matrix Metalloproteinase 9