Probiotic Properties ofLactobacillus plantarumRYPR1 from an Indigenous Fermented Beverage Raabadi.
Present study documents the potential probioticLactobacillusisolated from indigenous fermented beverage Raabadi, consumed during summers in Haryana and Rajasthan regions of India. A total of five Raabadi samples were collected aseptically and 54 isolates were purified using MRS medium. All the isolates were assessed for tolerance to low pH and bile salts. It was observed that out of 54 only 24 isolates could survive the simulated gastric conditions. These isolates were further evaluatedin vitrofor cell surface hydrophobicity, cell surface hydrophobicity, hypocholesteramic activity, anti-oxidative potential, BSH activity, antagonistic activity, and antibiotic resistance profile. In addition, the confirmation of phenol resistance was also done. On the basis of results obtained, the survival rate of isolates was noted and six isolates were finally selected for further studies. Among themLactobacillus plantarumRYPR1 and RYPC7 showed good survival at pH 2 which shows good acid tolerance. Moreover,L. plantarumRYPR1 showed the highest hydrophobicity (79.13%) and represented the deconjugation of bile salts, which help in their adhesion to epithelial cells and colonization. Furthermore, RYPR1 also exhibited highest cholesterol reduction (59%) and subsequent analysis of results revealed that the above mentioned isolates further exhibit a good hypocholesterolemic effect and could be possibly used to prevent hypercholesterolemia. The present study divulges thatL. plantarumRYPR1 has an excellent probiotic potential.
Enzyme Technology and Protein Bioinformatics Laboratory, Department of Microbiology, Maharshi Dayanand University Rohtak, India.
Ruby
R
Yadav
Division of Dairy Microbiology, ICAR-National Dairy Research InstituteKarnal, India; College of Dairy Science and Technology, Guru Angad Dev Veterinary and Animal Sciences UniversityLudhiana, India.
Anil K
AK
Puniya
Enzyme Technology and Protein Bioinformatics Laboratory, Department of Microbiology, Maharshi Dayanand University Rohtak, India.
Pratyoosh
P
Shukla
Lactobacillus plantarum; cell surface hydrophobicity; hypocholesterolemic effect; probiotics; raabadi