Genetic Fingerprinting Using Microsatellite Markers in a Multiplex PCR Reaction: A Compilation of Methodological Approaches from Primer Design to Detection Systems.
Microsatellites are polymorphic DNA loci comprising repeated sequence motifs of two to five base pairs which are dispersed throughout the genome. Genotyping of microsatellites is a widely accepted tool for diagnostic and research purposes such as forensic investigations and parentage testing, but also in clinics (e.g. monitoring of bone marrow transplantation), as well as for the agriculture and food industries. The co-amplification of several short tandem repeat (STR) systems in a multiplex reaction with simultaneous detection helps to obtain more information from a DNA sample where its availability may be limited. Here, we introduce and describe this commonly used genotyping technique, providing an overview on available resources on STRs, multiplex design, and analysis.
Department of Medicine, Dermatology and Neurology, University of Leipzig, Leipzig, Germany.|Leipzig University Medical Center, IFB Adiposity Diseases, University of Leipzig, Leipzig, Germany.
Jacqueline
J
Krüger
Department of Medicine, Dermatology and Neurology, University of Leipzig, Leipzig, Germany. dorit.schleinitz@medizin.uni-leipzig.de.|Leipzig University Medical Center, IFB Adiposity Diseases, University of Leipzig, Leipzig, Germany. dorit.schleinitz@medizin.uni-leipzig.de.
Dorit
D
Schleinitz
D016172:DNA Fingerprinting / Q000379:methods*; D017931:DNA Primers; D006801:Humans; D018895:Microsatellite Repeats; D060885:Multiplex Polymerase Chain Reaction / Q000379:methods*
D017931:DNA Primers
Capillary electrophoresis; DNA profiling; Genotyping; Multiplex PCR; NGS; Primer design; STR; WGA