Tetramethylpyrazine Effects on the Expression of Scar-Related Genes in Rabbit Benign Biliary Stricture Fibroblasts.
OBJECTIVE
To investigate the effects of tetramethylpyrazine (TMP) on transforming growth factor-β1 (TGF- β1), α-smooth muscle actin (α-SMA), and neuronal regeneration related protein (P311) in benign biliary stricture fibroblasts of rabbit.


METHODS
An experimental study.


METHODS
Guizhou Medical University, Guiyang, Guizhou, China, from April to December 2015.


METHODS
Fibroblasts isolated from rabbits following benign biliary stricture were cultured and treated with different concentrations of TMP(0.08, 0.4, and 2.0 mg/ml). TMP-treated cells and non-treated control groups were incubated for 48-hours, and proliferation was assessed using the cell counting kit-8 assay. The mRNAexpressions of TGF-β1, α-SMA, and P311 were assessed by quantitative RT-PCR. Protein expressions of TGF-β1 and α-SMAwere investigated by Western blotting.


RESULTS
Treatment with TMPsignificantly reduced the proliferation of benign biliary stricture fibroblasts, and significantly attenuated both the mRNAand protein expressions of TGF-β1, α-SMA, and P311 (p < 0.05) in a dose-dependent manner.


CONCLUSIONS
TMPsignificantly reduced the proliferation of benign biliary stricture fibroblasts, and significantly downregulated the mRNA/protein expression of TGF-β1, α-SMA, and P311. Therefore, TMPmay be a therapeutic option for the prevention of benign biliary stricture.
Graduate School of Tianjin Medical University, 300070, Tianjin, China.
Ke Yue
KY
Li
Department of Hepatobiliary Surgery, Guizhou Provincial People's Hospital, Guiyang 550002, Guizhou Province, China.
Cheng Xian
CX
Shi
Department of Hepatobiliary Surgery, Guizhou Provincial People's Hospital, Guiyang 550002, Guizhou Province, China.
Jian Zhao
JZ
Huang
Department of Hepatobiliary Surgery, Guizhou Provincial People's Hospital, Guiyang 550002, Guizhou Province, China.
Ke Li
KL
Tang
D000199:Actins / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D002921:Cicatrix / Q000378:metabolism / Q000473:pathology*; D003251:Constriction, Pathologic / Q000473:pathology; D005347:Fibroblasts / Q000187:drug effects* / Q000378:metabolism*; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism*; D015513:Oncogene Proteins / Q000235:genetics / Q000378:metabolism*; D011719:Pyrazines / Q000494:pharmacology*; D012333:RNA, Messenger / Q000235:genetics; D011817:Rabbits; D020133:Reverse Transcriptase Polymerase Chain Reaction; D053773:Transforming Growth Factor beta1 / Q000235:genetics / Q000378:metabolism*; D014665:Vasodilator Agents / Q000494:pharmacology*
C541116:ACTA2 protein, human; D000199:Actins; C428027:NREP protein, human; D009419:Nerve Tissue Proteins; D015513:Oncogene Proteins; D011719:Pyrazines; D012333:RNA, Messenger; D053773:Transforming Growth Factor beta1; D014665:Vasodilator Agents; C017953:tetramethylpyrazine