A Fluorescein-Based Colorimetric and Fluorescent Probe for Hydrazine and its Bioimaging in Live Cells.
A turn-on fluorescent probe (FN) for detection of hydrazine has been developed. Probe FN exhibited high selectivity and excellent sensitivity towards hydrazine with a detection limit as low as 4.6 × 10-10M. Probe FN selectively reacts with hydrazine (N2H4) in a physiological environment, leading to an off-on fluorescence response along with the color change from colorless to yellow, allowing colorimetric detection of hydrazine by the naked eye. Furthermore, probe FN was successfully applied for visualizing hydrazine in living cells.
School of Chemistry and Chemical Engineering, Xuchang University, Xuchang, 461000, China.
Gongchun
G
Li
Phosphorus Chemical Engineering Research Center of Henan Province, the College of Chemistry and Molecular Engineering, Zhengzhou University, Zhengzhou, 450052, China.
Yongxiang
Y
Liu
Phosphorus Chemical Engineering Research Center of Henan Province, the College of Chemistry and Molecular Engineering, Zhengzhou University, Zhengzhou, 450052, China.
Jianhua
J
Song
Phosphorus Chemical Engineering Research Center of Henan Province, the College of Chemistry and Molecular Engineering, Zhengzhou University, Zhengzhou, 450052, China. yeyong03@tsinghua.org.cn.
Yong
http://orcid.org/0000-0001-6234-3971
Y
Ye
D003124:Colorimetry / Q000379:methods*; D019793:Fluorescein / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry*; D006801:Humans; D006834:Hydrazines / Q000032:analysis*; D057230:Limit of Detection; D057054:Molecular Imaging / Q000379:methods*; D010190:Pancreatic Neoplasms / Q000378:metabolism*; D013050:Spectrometry, Fluorescence / Q000379:methods*; D014407:Tumor Cells, Cultured
D005456:Fluorescent Dyes; D006834:Hydrazines; C029424:hydrazine; D019793:Fluorescein
Fluorescein; Fluorescence; Hydrazine