In vitro susceptibility of the oomycete Pythium insidiosum to metallic compounds containing cadmium, lead, copper, manganese or zinc.
Pythium insidiosum is an aquatic oomycete that causes pythiosis, an important and severe disease of difficult treatment that affects humans, domestic and wild animals. This infection is often described in horses in Brazil and humans in Thailand. In clinical practice, we have observed many cases that do not respond to available therapies, indicating the need to explore alternative therapeutic approaches. In this sense, studies using metal compounds in conjunction with available antimicrobial agents have been demonstrated greater antimicrobial activity. Thus, in this research, we tested in vitro activities of metallic compounds containing cadmium, lead, copper, manganese, or zinc against 23 isolates of P. insidiosum. The assays were performed by broth microdilution based on CLSI M38-A2 document. The minimum inhibitory and fungicidal concentrations were established for all isolates. Copper acetate and cadmium acetate showed the highest inhibitory effects, with minimal inhibitory concentration ranging from 4-64 μg/ml and 16-256 μg/ml, respectively. The mean geometric for minimal fungicidal concentrations were, respectively, 26 μg/ml and 111.43 μg/ml for copper acetate and cadmium acetate. These results suggest that copper and cadmium can inhibit P. insidiosum growth, highlighting the greater inhibitory activity of copper acetate. In addition, our results propose that copper and/or cadmium compounds can be used in upcoming researches to formulate effective new complexed drugs against P. insidiosum in in vitro and in vivo experimental models.
Laboratório de Pesquisas Micológicas, Departamento de Microbiologia e Parasitologia, Universidade Federal de Santa Maria (UFSM), Campus Universitário, Prédio 20, 4139, 97105900, Santa Maria, RS, Brazil.
Tatiana Corrêa
TC
Ribeiro
Departamento de Medicina Veterinária Preventiva (DMVP), Programa de Pós-graduação em Medicina Veterinária (PPGMV), Centro de Ciências Rurais, UFSM, Santa Maria, RS, Brazil.
Carla
C
Weiblen
Departamento de Medicina Veterinária Preventiva (DMVP), Programa de Pós-graduação em Medicina Veterinária (PPGMV), Centro de Ciências Rurais, UFSM, Santa Maria, RS, Brazil.
Sônia de Avila
SA
Botton
Departamento de Microbiologia e Parasitologia, Instituto de Biologia, Universidade Federal de Pelotas (UFPel), Pelotas, RS, Brazil.
Daniela Isabel Brayer
DIB
Pereira
Laboratório de Pesquisas Micológicas, Departamento de Microbiologia e Parasitologia, Universidade Federal de Santa Maria (UFSM), Campus Universitário, Prédio 20, 4139, 97105900, Santa Maria, RS, Brazil.
Francielli Pantella Kunz
FPK
de Jesus
Laboratório de Pesquisas Micológicas, Departamento de Microbiologia e Parasitologia, Universidade Federal de Santa Maria (UFSM), Campus Universitário, Prédio 20, 4139, 97105900, Santa Maria, RS, Brazil.
Camila Marina
CM
Verdi
Departamento de Medicina Veterinária Preventiva (DMVP), Programa de Pós-graduação em Medicina Veterinária (PPGMV), Centro de Ciências Rurais, UFSM, Santa Maria, RS, Brazil.
Leticia Trevisan
LT
Gressler
Departamento de Medicina Veterinária Preventiva (DMVP), Programa de Pós-graduação em Medicina Veterinária (PPGMV), Centro de Ciências Rurais, UFSM, Santa Maria, RS, Brazil.
Luís Antonio
LA
Sangioni
Laboratório de Pesquisas Micológicas, Departamento de Microbiologia e Parasitologia, Universidade Federal de Santa Maria (UFSM), Campus Universitário, Prédio 20, 4139, 97105900, Santa Maria, RS, Brazil.
Janio Morais
JM
Santurio
D000085:Acetates / Q000494:pharmacology; D000935:Antifungal Agents / Q000737:chemistry / Q000494:pharmacology*; D002104:Cadmium / Q000494:pharmacology; D003300:Copper / Q000494:pharmacology; D066298:In Vitro Techniques; D008345:Manganese / Q000494:pharmacology; D008826:Microbial Sensitivity Tests; D009942:Organometallic Compounds / Q000494:pharmacology; D011775:Pythium / Q000187:drug effects* / Q000302:isolation & purification; D019287:Zinc Sulfate / Q000494:pharmacology
D000085:Acetates; D000935:Antifungal Agents; D009942:Organometallic Compounds; D002104:Cadmium; D008345:Manganese; D019287:Zinc Sulfate; D003300:Copper; C028031:cadmium acetate; C008261:lead acetate
cadmium; copper; metals; oomycetes; susceptibility test