The tenocyte phenotype of human primary tendon cells in vitro is reduced by glucocorticoids.
The use of corticosteroids (e.g., dexamethasone) as treatment for tendinopathy has recently been questioned as higher risks for ruptures have been observed clinically. In vitro studies have reported that dexamethasone exposed tendon cells, tenocytes, show reduced cell viability and collagen production. Little is known about the effect of dexamethasone on the characteristics of tenocytes. Furthermore, there are uncertainties about the existence of apoptosis and if the reduction of collagen affects all collagen subtypes.



We evaluated these aspects by exposing primary tendon cells to dexamethasone (Dex) in concentrations ranging from 1 to 1000 nM. Gene expression of the specific tenocyte markers scleraxis (Scx) and tenomodulin (Tnmd) and markers for other mesenchymal lineages, such as bone (Alpl, Ocn), cartilage (Acan, Sox9) and fat (Cebpα, Pparg) was measured via qPCR. Cell viability and proliferation was calculated using a MTS Assay. Cell death was measured by LDH assay and cleaved caspase-3 using Western Blot. Gene expression of collagen subtypes Col1, Col3 and Col14 was analyzed using qPCR.



Stimulation with Dex decreased cell viability and LDH levels. Dex also induced a significant reduction of Scx gene expression and a marked loss of fibroblast like cell shape. The mRNA for all examined collagen subtypes was found to be down-regulated. Among non-tendinous genes only Pparg was significantly increased, whereas Acan, Alpl and Sox9 were reduced.



These results indicate a Dex induced phenotype drift of the tenocytes by reducing scleraxis expression. Reduction of several collagen subtypes, but not cell death, seems to be a feature of Dex induced tissue degeneration.
Department of Integrative Medical Biology, Anatomy, Umeå University, SE-901 87, Umeå, Sweden. christoph.spang@umu.se.|Dr Alfen Orthopedic Spine Center, 97080, Würzburg, Germany. christoph.spang@umu.se.
Christoph
0000-0002-9977-334X
C
Spang
Department of Integrative Medical Biology, Anatomy, Umeå University, SE-901 87, Umeå, Sweden.
Jialin
J
Chen
Department of Integrative Medical Biology, Anatomy, Umeå University, SE-901 87, Umeå, Sweden.
Ludvig J
LJ
Backman
D051792:Basic Helix-Loop-Helix Proteins / Q000378:metabolism; D016923:Cell Death / Q000187:drug effects; D003094:Collagen / Q000378:metabolism; D003907:Dexamethasone / Q000009:adverse effects*; D005260:Female; D005938:Glucocorticoids / Q000009:adverse effects*; D006801:Humans; D008297:Male; D008565:Membrane Proteins / Q000378:metabolism; D008875:Middle Aged; D010641:Phenotype; D061251:Primary Cell Culture; D052256:Tendinopathy / Q000188:drug therapy*; D000070916:Tenocytes / Q000187:drug effects* / Q000378:metabolism
D051792:Basic Helix-Loop-Helix Proteins; D003094:Collagen; D003907:Dexamethasone; D005938:Glucocorticoids; D008565:Membrane Proteins; C093504:SCX protein, human; C422444:TNMD protein, human
Cell viability; Collagen; Dexamethasone; Phenotype; Scleraxis; Tendinopathy; Tendon cells; Tenocytes