Biomass-water interactions correlate to recalcitrance and are intensified by pretreatment: An investigation of water constraint and retention in pretreated spruce using low field NMR and water retention value techniques.
The underlying mechanisms of the recalcitrance of biomass to enzymatic deconstruction are still not fully understood, and this hampers the development of biomass based fuels and chemicals. With water being necessary for most biological processes, it is suggested that interactions between water and biomass may be key to understanding and controlling biomass recalcitrance. This study investigates the correlation between biomass recalcitrance and the constraint and retention of water by the biomass, using SO2pretreated spruce, a common feedstock for lignocellulosic biofuel production, as a substrate to evaluate this relationship. The water retention value (WRV) of the pretreated materials was measured, and water constraint was assessed using time domain Low Field Nuclear Magnetic Resonance (LFNMR) relaxometry. WRV increased with pretreatment severity, correlating to reduced recalcitrance, as measured by hydrolysis of cellulose using commercial enzyme preparations. Water constraint increased with pretreatment severity, suggesting that a higher level of biomass-water interaction is indicative of reduced recalcitrance in pretreated materials. Both WRV and water constraint increased significantly with reductions in particle size when pretreated materials were further milled, suggesting that particle size plays an important role in biomass water interactions. It is suggested that WRV may be a simple and effective method for measuring and comparing biomass recalcitrance. © 2016 American Institute of Chemical Engineers Biotechnol. Prog., 33:146-153, 2017.
Dept. of Geosciences and Natural Resource Management, University of Copenhagen, Copenhagen, Denmark.
Noah D
0000-0002-1446-3095
ND
Weiss
Dept. of Geosciences and Natural Resource Management, University of Copenhagen, Copenhagen, Denmark.
Lisbeth Garbrecht
LG
Thygesen
Dept. of Geosciences and Natural Resource Management, University of Copenhagen, Copenhagen, Denmark.
Claus
C
Felby
Dept. of Chemical Engineering, Lund University, Lund, Sweden.
Christian
C
Roslander
Dept. of Forest Products, Biotechnology and Bioenergy, University of British Columbia, Vancouver, Canada.
Keith
K
Gourlay
D056804:Biofuels; D018533:Biomass; D001709:Biotechnology / Q000379:methods; D002482:Cellulose / Q000737:chemistry*; D006868:Hydrolysis; D008031:Lignin / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D014867:Water / Q000737:chemistry*
D056804:Biofuels; D014867:Water; D002482:Cellulose; D008031:Lignin
biomass recalcitrance; biomass water interactions; low field NMR; pretreatment; water retention value