Adaptive illumination based on direct wavefront sensing in a light-sheet fluorescence microscope.
A methodology for the adaptive control and correction of phase aberrations in the illumination arm of a light-sheet fluorescence microscope has been developed. The method uses direct wavefront sensing on epi-fluorescent light to detect the aberration present in the sample. Using this signal, the aberrations in the illumination arm are subsequently corrected with a spatial light modulator in a feedforward mode. Adaptive correction, resulting in significant improvement in the axial resolution, has been demonstrated by imaging Tg(fli:GFP) zebrafish embryos.
Dean
D
Wilding
Paolo
P
Pozzi
Oleg
O
Soloviev
Gleb
G
Vdovin
Michel
M
Verhaegen
D000818:Animals; D004625:Embryo, Nonmammalian; D005453:Fluorescence; D008856:Microscopy, Fluorescence; D055095:Optics and Photonics; D015027:Zebrafish