Electrospun Lipid Binding Proteins Composite Nanofibers with Antibacterial Properties.
Electrospinning is here used for the first time to prepare nanofibers including a host/guest complex in a keratin/poly(ethylene oxide) matrix. The host is a lipid binding protein and the guest is an insoluble bactericidal molecule, irgasan, bound within the protein internal cavity. The obtained nanofibers, characterized by scanning electron microscopy, exhibit excellent antibacterial activity toward Gram positive and negative bacteria, even with a moderate protein/irgasan cargo. Solution NMR studies, employed to provide molecular information on the cargo system, points to a micromolar affinity, compatible with both the electrospinning process and slow guest release. The versatility of the carrier protein, capable of interacting with a variety of druggable hydrophobic molecules, is exploitable for the development of innovative biomedical devices, whose properties can be tuned by the selected guest.
Istituto per lo Studio delle Macromolecole (ISMAC), CNR, via Corti 12, 20133, Milano, Italy.
Simona
S
Tomaselli
Istituto per lo Studio delle Macromolecole (ISMAC), CNR, C.so G. Pella 16, 13900, Biella, Italy.
Diego Omar Sanchez
DO
Ramirez
Istituto per lo Studio delle Macromolecole (ISMAC), CNR, C.so G. Pella 16, 13900, Biella, Italy.
Riccardo Andrea
RA
Carletto
Istituto per lo Studio delle Macromolecole (ISMAC), CNR, C.so G. Pella 16, 13900, Biella, Italy.
Alessio
A
Varesano
Istituto per lo Studio delle Macromolecole (ISMAC), CNR, C.so G. Pella 16, 13900, Biella, Italy.
Claudia
C
Vineis
Dipartimento di Biotecnologie, Università degli Studi di Verona, Strada le Grazie 15, 37134, Verona, Italy.
Serena
S
Zanzoni
Istituto per lo Studio delle Macromolecole (ISMAC), CNR, via Corti 12, 20133, Milano, Italy.
Henriette
H
Molinari
Istituto per lo Studio delle Macromolecole (ISMAC), CNR, via Corti 12, 20133, Milano, Italy.
Laura
L
Ragona
D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002224:Carbanilides / Q000494:pharmacology; D002352:Carrier Proteins / Q000378:metabolism*; D002645:Chickens; D004926:Escherichia coli / Q000187:drug effects; D008055:Lipids / Q000737:chemistry*; D008562:Membrane Glycoproteins / Q000378:metabolism*; D008826:Microbial Sensitivity Tests; D057139:Nanofibers / Q000737:chemistry* / Q000648:ultrastructure; D036103:Nanotechnology / Q000379:methods*; D066244:Proton Magnetic Resonance Spectroscopy; D012756:Sheep; D012996:Solutions; D013211:Staphylococcus aureus / Q000187:drug effects; D017155:Wettability
D000900:Anti-Bacterial Agents; D002224:Carbanilides; D002352:Carrier Proteins; D008055:Lipids; D008562:Membrane Glycoproteins; D012996:Solutions; C037643:bile acid binding proteins; C005055:cloflucarban
NMR; electrospun nanofiber; irgasan; lipid binding protein; wound dressing