Molecular evidence of high-risk human papillomavirus infection in colorectal tumours from Cuban patients.
The association between colorectal cancer and human papillomavirus (HPV) infection is still unproven. The aim of this study was to investigate the presence of high-risk HPV (HR-HPV) DNA in colorectal tissues from Cuban patients. A total of 63 colorectal formalin-fixed paraffin-embedded tissues were studied (24 adenocarcinoma, 18 adenoma, and 21 colorectal tissues classified as benign colitis). DNA from colorectal samples was analysed by quantitative real-time polymerase chain reaction to detect the most clinically relevant high HR-HPV types (HPV-16, -18, -31, -33, -45, -52, and -58). Associations between histologic findings and other risk factors were also analysed. Overall, HPV DNA was detected in 23.8% (15/63) of the samples studied. Viral infections were detected in 41.7% of adenocarcinoma (10/24) and 27.7% of adenoma cases (5/18). HPV DNA was not found in any of the negative cases. An association between histological diagnosis of adenocarcinoma and HPV infection was observed (odd ratio = 4.85, 95% confidence interval = 1.40-16.80, p = 0.009). The only genotypes identified were HPV 16 and 33. Viral loads were higher in adenocarcinoma, and these cases were associated with HPV 16. This study provides molecular evidence of HR-HPV infection in colorectal adenocarcinoma tissues from Cuban patients.
Institute of Tropical Medicine Pedro Kourí, Department of Virology, Laboratory of Sexually Transmitted Diseases,La Habana, Cuba.
Yudira
Y
Soto
Institute of Tropical Medicine Pedro Kourí, Department of Virology, Laboratory of Sexually Transmitted Diseases,La Habana, Cuba.
Celia Maria
CM
Limia
Institute of Gastroenterology, La Habana, Cuba.
Licet
L
González
Institute of Gastroenterology, La Habana, Cuba.
Bienvenido
B
Grá
Institute of Gastroenterology, La Habana, Cuba.
Olga Marina
OM
Hano
Institute of Tropical Medicine Pedro Kourí, Department of Virology, Laboratory of Sexually Transmitted Diseases,La Habana, Cuba.
Pedro Ariel
PA
Martínez
Institute of Tropical Medicine Pedro Kourí, Department of Virology, Laboratory of Sexually Transmitted Diseases,La Habana, Cuba.
Vivian
V
Kourí
D000230:Adenocarcinoma / Q000821:virology*; D000236:Adenoma / Q000821:virology*; D000328:Adult; D015179:Colorectal Neoplasms / Q000821:virology*; D003462:Cuba; D004279:DNA, Viral / Q000032:analysis*; D005260:Female; D005838:Genotype; D006801:Humans; D008297:Male; D008875:Middle Aged; D027383:Papillomaviridae / Q000235:genetics*; D030361:Papillomavirus Infections / Q000150:complications*; D060888:Real-Time Polymerase Chain Reaction; D019562:Viral Load
D004279:DNA, Viral