Micrococcal Nuclease Digestion of Schizosaccharomyces pombe Chromatin.
Digestion of chromatin with micrococcal nuclease (MNase) is widely used to probe nucleosome organization. Analysis of MNase digests by end-labeling techniques or overlapping quantitative polymerase chain reaction (qPCR) can be used to map locus-specific nucleosome positions. Furthermore, the application of genomic technologies can provide genome-wide views of nucleosome position and occupancy. This protocol provides a basic method for MNase digestion of Schizosaccharomyces pombe chromatin and depends on the production of permeabilized spheroplasts.
Biology Department, Boston College, Chestnut Hill, Massachusetts 02467.
Hugh P
HP
Cam
Institute for Cell and Molecular Biosciences, Newcastle University, Newcastle NE2 4HH, United Kingdom.
Simon
S
Whitehall
D002843:Chromatin / Q000378:metabolism*; D006868:Hydrolysis; D008836:Micrococcal Nuclease / Q000378:metabolism*; D009707:Nucleosomes / Q000737:chemistry*; D012568:Schizosaccharomyces / Q000737:chemistry / Q000235:genetics*
D002843:Chromatin; D009707:Nucleosomes; D008836:Micrococcal Nuclease