L-cysteine capped lanthanum hydroxide nanostructures for non-invasive detection of oral cancer biomarker.
In this paper, we present the result of studies related to the in situ synthesis of amino acid (L-Cysteine) capped lanthanum hydroxide nanoparticles [Cys-La(OH)3NPs] towards the fabrication of efficient immunosensor for non-invasive detection of oral cancer. The characterization of Cys-La(OH)3NPs was carried out by different techniques including X-ray diffraction, scanning electron microscopy, transmission electron microscopy, fourier transform infrared spectroscopy and electrochemical techniques. These Cys-La(OH)3NPs were electrophoretically deposited onto an indium-tin-oxide glass substrate and used for immobilization of anti-cytokeratin fragment-21-1 (anti-Cyfra-21-1) for the electrochemical detection of Cyfra-21-1. This immunosensor shows a broad detection range of 0.001-10.2ngmL-1, the low detection limit of 0.001ngmL-1, and high sensitivity of 12.044µA (ng per mL cm-2)-1with a response time of 5min. This immunosensor was found to be more advanced in terms of high sensitivity and low detection limit as compared to previously reported biosensors and commercially available ELISA kit (Kinesis DX).
Special Centre for Nanosciences, Jawaharlal Nehru University, New Delhi 110067, India.
Sachchidanand
S
Tiwari
Special Centre for Nanosciences, Jawaharlal Nehru University, New Delhi 110067, India.
Pramod K
PK
Gupta
Special Centre for Nanosciences, Jawaharlal Nehru University, New Delhi 110067, India.
Yana
Y
Bagbi
Special Centre for Nanosciences, Jawaharlal Nehru University, New Delhi 110067, India.
Tamal
T
Sarkar
Special Centre for Nanosciences, Jawaharlal Nehru University, New Delhi 110067, India. Electronic address: pratimarsolanki@gmail.com.
Pratima R
PR
Solanki
D000951:Antigens, Neoplasm / Q000737:chemistry / Q000302:isolation & purification*; D014408:Biomarkers, Tumor / Q000737:chemistry / Q000302:isolation & purification*; D003545:Cysteine / Q000737:chemistry*; D005898:Glass / Q000737:chemistry; D006801:Humans; D007204:Indium / Q000737:chemistry; D053539:Keratin-19 / Q000737:chemistry / Q000302:isolation & purification*; D007811:Lanthanum / Q000737:chemistry; D009062:Mouth Neoplasms / Q000175:diagnosis*; D049329:Nanostructures / Q000737:chemistry; D017971:Tin Compounds / Q000737:chemistry
D000951:Antigens, Neoplasm; D014408:Biomarkers, Tumor; D053539:Keratin-19; D017971:Tin Compounds; C102012:antigen CYFRA21.1; D007204:Indium; C033562:lanthanum hydroxide; D007811:Lanthanum; D003545:Cysteine; C045358:stannic oxide
Anti-Cyfra-21-1; Cyfra-21-1; Immunosensor; L-Cysteine, Lanthanum hydroxide; Oral cancer