Comparative Analysis of HaSNPV-AC53 and Derived Strains.
Complete genome sequences of two Australian isolates ofH. armigerasingle nucleopolyhedrovirus (HaSNPV) and nine strains isolated by plaque selection in tissue culture identified multiple polymorphisms in tissue culture-derived strains compared to the consensus sequence of the parent isolate. Nine open reading frames (ORFs) in all tissue culture-derived strains contained changes in nucleotide sequences that resulted in changes in predicted amino acid sequence compared to the parent isolate. Of these, changes in predicted amino acid sequence of six ORFs were identical in all nine derived strains. Comparison of sequences and maximum likelihood estimation (MLE) of specific ORFs and whole genome sequences were used to compare the isolates and derived strains to published sequence data from other HaSNPV isolates. The Australian isolates and derived strains had greater sequence similarity to New World SNPV isolates fromH. zeathan to Old World isolates fromH. armigera, but with characteristics associated with both. Three distinct geographic clusters within HaSNPV genome sequences were identified: Australia/Americas, Europe/Africa/India, and China. Comparison of sequences and fragmentation of ORFs suggest that geographic movement and passage in vitro result in distinct patterns of baculovirus strain selection and evolution.
Queensland University of Technology, Brisbane 4000, Australia. chris.noune@connect.qut.edu.au.
Christopher
C
Noune
Queensland University of Technology, Brisbane 4000, Australia. caroline.hauxwell@qut.edu.au.
Caroline
C
Hauxwell
D000818:Animals; D001315:Australia; D016000:Cluster Analysis; D014644:Genetic Variation; D007915:Lepidoptera / Q000821:virology*; D020125:Mutation, Missense; D017924:Nucleopolyhedroviruses / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D016366:Open Reading Frames; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D010948:Viral Plaque Assay; D014776:Virus Cultivation
Helicoverpa; Next Generation Sequencing; SNPV; baculovirus; strain selection; virus evolution