A Multiplex Ligation Assay for miRNA Copy Number Profiling.
Ligo-miR is an assay technology that can perform multiplexed detection of miRNAs from a wide range of biological sources. At its core are two sequential ligation steps. First in the capture ligation, template molecules are created by ligating a DNA adapter to the 3' end of all miRNA molecules. Then in the coding ligation these templates are used to generate, linearly amplified, DNA products encoded by length. The resultant number of each DNA product is proportional to the original number of miRNA molecules. The products and their corresponding miRNA can be identified and quantified using common DNA sizing methods such as electrophoresis.
Circulomics Inc., 810 Wyman Park Drive, Unit G01, Baltimore, MD, 21211, USA. dkilburn@circulomics.com.
Duncan
D
Kilburn
Biomedical Engineering Department, Johns Hopkins University, Baltimore, MD, USA.
Yunke
Y
Song
Biomedical Engineering Department, Johns Hopkins University, Baltimore, MD, USA.|Mechanical Engineering Department, Johns Hopkins University, Baltimore, MD, USA.|Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University, Baltimore, MD, USA.|Center of Cancer Nanotechnology Excellence, Johns Hopkins University, Baltimore, MD, USA.
Tza-Huei
TH
Wang
Circulomics Inc., 810 Wyman Park Drive, Unit G01, Baltimore, MD, 21211, USA.
Kelvin J
KJ
Liu
D002478:Cells, Cultured; D020869:Gene Expression Profiling / Q000379:methods*; D006801:Humans; D035683:MicroRNAs / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism
D035683:MicroRNAs
Copy number; Ligation; Multiplex; Profiling; Quantification assay; miRNA