Taiwanin E inhibits cell migration in human LoVo colon cancer cells by suppressing MMP-2/9 expression via p38 MAPK pathway.
Taiwanin E is a natural compound which is structurally analogous to estrogen II and is abundantly found in Taiwania cryptomerioides. It has been previously reported for its anticancer effects; however, the pharmaceutical effect of Taiwanin E on Human LoVo colon cancer cells is not clear. In this study, we investigated the effects of Taiwanin E on metastasis and the associated mechanism of action on Human LoVo colon cancer cells with respect to the modulations in their cell migration and signaling pathways associated with migration. The results showed that Taiwanin E inhibited cell migration ability correlated with reduced expression and activity of MMP-2 and MMP-9. In addition, Taiwanin E induced activation of p38 through phosphorylation. Inhibition of p38α/β significantly abolished the effect of Taiwanin E on cell migration and MMP-2/-9 activity. Our results conclude that Taiwanin E inhibited cell migration chiefly via p38α MAPK pathway and in a lesser extend via p38β MAPK. The results elucidate the potential of the phytoestrogen natural compound Taiwanin E as a cancer therapeutic agent in inhibiting the cell migration. © 2016 Wiley Periodicals, Inc. Environ Toxicol 32: 2021-2031, 2017.
Division of Colorectal Surgery, Mackay Memorial Hospital, Taipei, Taiwan.|Nursing Division, Mackay Medicine, Nursing and Management College, Taipei, Taiwan.
Hsi-Hsien
HH
Hsu
Department of Biological Science and Technology, China Medical University, Taichung, Taiwan.
Wei-Wen
WW
Kuo
Department of Nursing, Mei Ho University, Pingguang Road, Pingtung, Taiwan.
Cecilia Hsuan
CH
Day
Graduate Institute of Basic Medical Science, China Medical University, Taichung, Taiwan.
Marthandam Asokan
MA
Shibu
Graduate Institute of Basic Medical Science, China Medical University, Taichung, Taiwan.
Shin-Yi
SY
Li
Department of Health, Executive Yuan, Tsao-Tun Psychiatric Center, Nantou, Taiwan.
Sheng-Huang
SH
Chang
Graduate Institute of Basic Medical Science, China Medical University, Taichung, Taiwan.
Hui-Nung
HN
Shih
Department of Surgery, School of Medicine, College of Medicine, Taipei Medical University, Taipei, Taiwan.
Ray-Jade
RJ
Chen
Department of Biotechnology, Bharathiar University, Coimbatore, 641 046, India.
Vijaya Padma
VP
Viswanadha
Department of Chinese Pharmaceutical Sciences and Chinese Medicine Resources, China Medical University, Taichung, Taiwan.
Yueh-Hsiung
YH
Kuo
Graduate Institute of Basic Medical Science, China Medical University, Taichung, Taiwan.|Graduate Institute of Chinese Medical Science, China Medical University, Taichung, Taiwan.|Department of Health and Nutrition Biotechnology, Asia University, Taichung, Taiwan.
Chih-Yang
CY
Huang
D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects; D003110:Colonic Neoplasms; D006801:Humans; D017705:Lignans / Q000494:pharmacology*; D020935:MAP Kinase Signaling System; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism*; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism*; D010766:Phosphorylation; D048051:p38 Mitogen-Activated Protein Kinases / Q000378:metabolism*
D000972:Antineoplastic Agents, Phytogenic; D017705:Lignans; C000602275:taiwanin E; D048051:p38 Mitogen-Activated Protein Kinases; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9
Taiwania cryptomerioides Hayata; cell migration; colon cancer; matrix metalloproteinases; p38 MAPK