Identification of a new small bioactive peptide from Lactobacillus gasseri supernatant.
Ten lactic acid bacteria (LAB) strains, previously isolated from human ileal biopsy of healthy volunteers, were screened for production and secretion of molecules having anti-bacterial and anti-biofilm activities. Because many recent reports indicate that LAB secreted molecules may exert immune-modulatory action, we also tested the effect on human intestinal HCT116 cells challenged with bacterial lipopolysaccharides. One of the Lactobacillus gasseri strains, SF1109, strongly inhibited: (1) Pseudomonas aeruginosa growth; (2) Escherichia coli biofilm production; (3) LPS induction of P-ERK1/2 in HCT116 cells, and was selected for further characterisation of the secreted active molecule. Cell-free supernatant of the L. gasseri SF1109 was analysed and one 1.3 kDa peptide has been characterised. Eight out twelve amino acids of this peptide were identified allowing the synthesis of an octa-peptide which still presented the mentioned activities.
1 Department of Biology, University of Naples Federico II, Via Cintia, 80126 Naples, Italy.
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Zanfardino
1 Department of Biology, University of Naples Federico II, Via Cintia, 80126 Naples, Italy.
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Criscuolo
1 Department of Biology, University of Naples Federico II, Via Cintia, 80126 Naples, Italy.
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B
Di Luccia
1 Department of Biology, University of Naples Federico II, Via Cintia, 80126 Naples, Italy.
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Pizzo
2 CNR - Institute of Biomolecular Chemistry, Via Campi Flegrei 34, 80078 Pozzuoli, Italy.
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Ciavatta
1 Department of Biology, University of Naples Federico II, Via Cintia, 80126 Naples, Italy.
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Notomista
3 Department of Chemical Sciences, University of Naples Federico II, Via Cintia, 80126 Naples, Italy.
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Carpentieri
3 Department of Chemical Sciences, University of Naples Federico II, Via Cintia, 80126 Naples, Italy.
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Pezzella
1 Department of Biology, University of Naples Federico II, Via Cintia, 80126 Naples, Italy.
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M
Varcamonti
D000890:Anti-Infective Agents / Q000494:pharmacology; D018441:Biofilms / Q000187:drug effects* / Q000254:growth & development; D004926:Escherichia coli / Q000187:drug effects* / Q000254:growth & development; D045325:HCT116 Cells; D006801:Humans; D007155:Immunologic Factors / Q000378:metabolism; D056747:Immunomodulation; D007422:Intestines / Q000382:microbiology; D000069998:Lactobacillus gasseri / Q000737:chemistry*; D009894:Opportunistic Infections / Q000382:microbiology / Q000517:prevention & control*; D010455:Peptides / Q000302:isolation & purification* / Q000494:pharmacology; D011550:Pseudomonas aeruginosa / Q000187:drug effects* / Q000254:growth & development
D000890:Anti-Infective Agents; D007155:Immunologic Factors; D010455:Peptides
antimicrobial molecule; intestinal bacteria; opportunistic pathogens