Direct evidence for the semipersistent transmission of Cucurbit chlorotic yellows virus by a whitefly vector.
Cucurbit chlorotic yellows virus (CCYV) (genus Crinivirus, family Closteroviridae) is an emerging plant virus, and is now spreading and causing severe economic losses to cucurbit crops in many Asian countries. CCYV is believed to be transmitted specifically by the sweetpotato whitefly, Bemisia tabaci, in a semipersistent manner. In the present study, we provide direct evidence for the semipersistent transmission of CCYV by Mediterranean (MED) cryptic species of B. tabaci complex. We investigated CCYV transmission characteristics, and immunofluorescently labeled and localized the virus retention site within the vector by laser confocal microscopy. Whiteflies required ≥1 h of acquisition access period (AAP) to successfully acquire CCYV, and the proportion of RT-PCR positive whitefly individuals reached to 100% at 48 h of AAP. CCYV virons could be retained within vectors as long as 12 d, but the proportion of RT-PCR positive whiteflies dropped to 55% by 3 d. Groups of thirty whiteflies given a 24 h of inoculation access period (IAP) to inoculate CCYV on cucumber plants showed a transmission efficiency rate of 72.73%. The retention site of CCYV virons was located in the foregut of virion-fed vectors. These results definitely indicated the semipersistent transmission mode of CCYV by B. tabaci MED.
College of Plant Protection, Henan Agricultural University, Zhengzhou, Henan 450002, China.
Jingjing
J
Li
College of Plant Protection, Henan Agricultural University, Zhengzhou, Henan 450002, China.
Xiangzhi
X
Liang
College of Plant Protection, Henan Agricultural University, Zhengzhou, Henan 450002, China.
Xueli
X
Wang
College of Plant Protection, Henan Agricultural University, Zhengzhou, Henan 450002, China.
Yan
Y
Shi
Zhengzhou Fruit Research Institute, Chinese Academy of Agricultural Sciences, Zhengzhou, Henan 450009, China.
Qinsheng
Q
Gu
Department of Plant Pathology, University of California, Davis, One shields Avenue, Davis, CA 95616-8600, USA.
Yen-Wen
YW
Kuo
Department of Plant Pathology, University of California, Davis, One shields Avenue, Davis, CA 95616-8600, USA.
Bryce W
BW
Falk
College of Plant Protection, Henan Agricultural University, Zhengzhou, Henan 450002, China.
Fengming
F
Yan
D000818:Animals; D029383:Crinivirus / Q000235:genetics / Q000302:isolation & purification* / Q000472:pathogenicity; D018556:Crops, Agricultural / Q000821:virology; D019662:Cucurbitaceae / Q000821:virology*; D006430:Hemiptera / Q000821:virology*; D007303:Insect Vectors; D012367:RNA, Viral / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction
D012367:RNA, Viral