Phytosterols fromSpondias mombinLinn with Antimycobacterial Activities.
The growing problems of tuberculosis have led to the search for new anti-Mtb agents from higher plants. The stem bark ofSpondias mombinwas evaluated for itsin vitroactivity againstMycobacterium tuberculosis(H37Rv strain). Bioassay-guided fractionation of the methanol extract was carried out by Vacuum Liquid Chromatography (VLC) on Silica gel (230-400 mesh) and purification was done using HPLC and TLC.In vitroantimycobacterial susceptibility was performed by a fluorometric microplate alamar blue assay (MABA) and percentage mycobacterial inhibition was calculated. The structures of the isolated compounds were established by spectroscopic analysis. The active VLC fraction exhibited 91% inhibition againstM. tuberculosisH37Rv at a concentration of 40 μg/mL. The HPLC fraction SMi-15 containing compounds1and2showed 92.8% inhibition againstM. tuberculosis. Two new antimycobacterial phytosterols were isolated from the stem bark ofS. mombinand the structures were identified as mombintane I (1) and mombintane II (2). The stem bark extractives ofS. mombincontain antitubercular principles of the class phytosterol and support an important potential of triterpenoids.
Department of Chemistry, Covenant University, P.M.B. 1023 Ota; Nigeria.
J A O
JA
Olugbuyiro
Department of Pharmacognosy, University of Ibadan, Ibadan; Nigeria.
J O
JO
Moody
Department of Pharmacognosy, and National Center for Natural Products Research, School of Pharmacy, The University of Mississippi, University, MS 38677, U.S.A.
M T
MT
Hamann
Mycobacterium tuberculosis; Spondias mombin; antimycobacterial; mombintane I; mombintane II