Evidence that ferritin is associated with light production in the mucus of the marine worm Chaetopterus.
The blue glow of the mucus from Chaetopterus involves a photoprotein, iron and flavins. Identity and respective role of these components remain, however, largely unresolved today, likely because of viscosity issues and inhibition of this system by oxidizers conventionally used to track bioluminescence activity. Here, we used gentle centrifugation to obtain a mucus supernatant showing no inhibition to oxidizers, allowing for further analysis. We applied conventional chromatographic techniques to isolate major proteins associated with light emission. Luminescence ability of elutriate fractions was tested with hydrogen peroxide to track photoprotein and/or protein-bound chromophore. Fractions producing light contained few major proteins, one with similarity to ferritin. Addition to the mucus of elements with inhibitory/potentiary effect on ferritin ferroxidase activity induced corresponding changes in light production, emphasizing the possible role of ferritin in the worm bioluminescence. DNA of the protein was cloned, sequenced, and expressed, confirming its identity to a Chaetopterus Ferritin (ChF). Both ferric and ferrous iron were found in the mucus, indicating the occurrence of both oxidase and reductase activity. Biochemical analysis showed ChF has strong ferroxidase activity, which could be a source of biological iron and catalytic energy for the worm bioluminescence when coupled to a reduction process with flavins.
Scripps Institution of Oceanography, Marine Biology Research Division, University of California San Diego, 9500 Gillman Drive, La Jolla, CA 92093, USA.
Renu
R
Rawat
Scripps Institution of Oceanography, Marine Biology Research Division, University of California San Diego, 9500 Gillman Drive, La Jolla, CA 92093, USA.
Dimitri D
DD
Deheyn
D000818:Animals; D002498:Centrifugation; D002570:Ceruloplasmin / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D003001:Cloning, Molecular; D005293:Ferritins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D006861:Hydrogen Peroxide / Q000494:pharmacology; D007501:Iron / Q000032:analysis; D049449:Luminescence; D008164:Luminescent Proteins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D009093:Mucus / Q000737:chemistry*; D011077:Polychaeta
D008164:Luminescent Proteins; D005293:Ferritins; D006861:Hydrogen Peroxide; D007501:Iron; D002570:Ceruloplasmin