Molecular hydrogen decelerates rheumatoid arthritis progression through inhibition of oxidative stress.
Rheumatoid arthritis (RA) is a chronic inflammatory disease which results in progressive destruction of the joint. In this study, we examined if the hydrogen could inhibit inflammation in a mouse model of collagen-induced arthritis (CIA) via oxidative stress on RA-FLSs. Moreover, to identify the mechanisms of action, we evaluated the effect of hydrogen on RA-FLSs development and the expression of pro-inflammatory cytokines and signaling pathways. Based on our result, H2enriched medium can increase super oxide dismutase (SOD) level following H2O2treatment and decrease 8-hydroxy-2'-deoxyguanosine (8-OHdG) level. Since H2O2treatment activates MAPK, NF-κB and TGF-β1 in cells, our study suggested that H2could inhibit H2O2activated MAPK and NF-κB activation as well as TGF-β1 expression in treated cells. Taken together, our data suggested that H2can directly neutralize OH and ONOO-to reduce oxidative stress. Moreover, MAPK and NF-κB pathway also play roles in oxidative damage caused by H2O2in RA-FLSs. H2can provide protection to cells against inflammation, which may be related to inhibition of the activation of MAPK and NF-κB.
Department of Orthopedics, Jinling Hospital, Clinical School of Nanjing, Second Military Medical University 305 Zhongshan East Road, Nanjing, Jiangsu, China.
Jia
J
Meng
Department of Burn and Plastic Surgery, Jinling Hospital, School of Medicine, Nanjing University Nanjing 210002, Jiangsu, China.
Pan
P
Yu
Department of Orthopedics, Jinling Hospital, Clinical School of Nanjing, Second Military Medical University 305 Zhongshan East Road, Nanjing, Jiangsu, China.
Hui
H
Jiang
Department of Orthopedics, Jinling Hospital, Clinical School of Nanjing, Second Military Medical University 305 Zhongshan East Road, Nanjing, Jiangsu, China.
Tao
T
Yuan
Department of Orthopedics, Jinling Hospital, Clinical School of Nanjing, Second Military Medical University 305 Zhongshan East Road, Nanjing, Jiangsu, China.
Naicheng
N
Liu
Department of Orthopedics, Jinling Hospital, Clinical School of Nanjing, Second Military Medical University 305 Zhongshan East Road, Nanjing, Jiangsu, China.
Jian
J
Tong
Department of Joint, Guanghua Integrative Medicine Hospital 540 Xinhua Road, Shanghai 200025, China.
Haiyan
H
Chen
Department of Orthopedics, Jinling Hospital, Clinical School of Nanjing, Second Military Medical University 305 Zhongshan East Road, Nanjing, Jiangsu, China.
Nirong
N
Bao
Department of Orthopedics, Jinling Hospital, Clinical School of Nanjing, Second Military Medical University 305 Zhongshan East Road, Nanjing, Jiangsu, China.
Jianning
J
Zhao
NF-κB; Rheumatoid arthritis; SOD; TGF-β1; hydrogen