Localization and expression pattern of amelotin, odontogenic ameloblast-associated protein and follicular dendritic cell-secreted protein in the junctional epithelium of inflamed gingiva.
The purpose of this study is to elucidate the localization of amelotin (AMTN), odontogenic ameloblast-associated protein (ODAM) and follicular dendritic cell-secreted protein (FDC-SP) at the junctional epithelium (JE) in Porphyromonas gingivalis and Aggregatibacter actinomycetemcomitans infected mice and inflamed and non-inflamed human gingiva. We performed immunostaining to determine the localization and expression pattern of AMTN, ODAM and FDC-SP. AMTN, ODAM and FDC-SP in A. actinomycetemcomitans infected mice did not change dramatically compared with non-infected mice. AMTN and FDC-SP expressions were observed stronger in P. gingivalis infected mice at early stage. However, at the following stage, the coronal part of the AMTN expression disappeared from the JE, and FDC-SP expression decreased due to severe inflammation by P. gingivalis. ODAM expressed internal and external basal lamina, and the expression increased not only at early stage but also at the following stage in the inflammatory JE induced by P. gingivalis. In the human gingival tissues, AMTN was detected at the surface of the sulcular epithelium and JE in the non-inflamed and inflamed gingiva, and the localization did not change the process of inflammation. ODAM and FDC-SP were more widely detected at the sulcular epithelium and JE in the non-inflamed gingiva. In the inflamed gingiva, localization of ODAM and FDC-SP was spread into the gingival epithelium, compared to AMTN. These studies demonstrated that the expression pattern of AMTN, ODAM and FDC-SP at the JE were changed during inflammation process and these three proteins might play an important role in the resistance to inflammation.
Department of Periodontology and Research Institute of Oral Science, Nihon University School of Dentistry at Matsudo, Chiba, 271-8587, Japan.|Research Institute of Oral Science, Nihon University School of Dentistry at Matsudo, Chiba, 271-8587, Japan.
Yohei
Y
Nakayama
Department of Oral Immunology, Nihon University School of Dentistry at Matsudo, Chiba, 271-8587, Japan.
Ryoki
R
Kobayashi
Department of Periodontology and Research Institute of Oral Science, Nihon University School of Dentistry at Matsudo, Chiba, 271-8587, Japan.
Sari
S
Matsui
Department of Periodontology and Research Institute of Oral Science, Nihon University School of Dentistry at Matsudo, Chiba, 271-8587, Japan.
Hiroyoshi
H
Matsumura
Department of Periodontology and Research Institute of Oral Science, Nihon University School of Dentistry at Matsudo, Chiba, 271-8587, Japan.
Yasunobu
Y
Iwai
Department of Periodontology and Research Institute of Oral Science, Nihon University School of Dentistry at Matsudo, Chiba, 271-8587, Japan.
Keisuke
K
Noda
Department of Periodontology and Research Institute of Oral Science, Nihon University School of Dentistry at Matsudo, Chiba, 271-8587, Japan.
Mizuho
M
Yamazaki
Research Institute of Oral Science, Nihon University School of Dentistry at Matsudo, Chiba, 271-8587, Japan.|Department of Oral Immunology, Nihon University School of Dentistry at Matsudo, Chiba, 271-8587, Japan.
Tomoko
T
Kurita-Ochiai
Department of Periodontology, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, 852-8588, Japan.
Atsutoshi
A
Yoshimura
Tissue Regeneration, Department of Bio-Matrix, Tokyo Medical and Dental University, Tokyo, 113-8549, Japan.
Tamayuki
T
Shinomura
Matrix Dynamics Group, Faculty of Dentistry, University of Toronto, Toronto, ON, Canada.
Bernhard
B
Ganss
Department of Periodontology and Research Institute of Oral Science, Nihon University School of Dentistry at Matsudo, Chiba, 271-8587, Japan. ogata.yorimasa@nihon-u.ac.jp.|Research Institute of Oral Science, Nihon University School of Dentistry at Matsudo, Chiba, 271-8587, Japan. ogata.yorimasa@nihon-u.ac.jp.
Yorimasa
Y
Ogata
D016976:Aggregatibacter actinomycetemcomitans; D000818:Animals; D016866:Bacteroidaceae Infections / Q000378:metabolism*; D003746:Dental Enamel Proteins / Q000378:metabolism*; D004195:Disease Models, Animal; D004846:Epithelial Attachment / Q000378:metabolism*; D005881:Gingiva / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry; D007249:Inflammation / Q000378:metabolism; D047908:Intracellular Signaling Peptides and Proteins; D051379:Mice; D016871:Pasteurellaceae Infections / Q000378:metabolism*; D010518:Periodontitis / Q000378:metabolism*; D016966:Porphyromonas gingivalis; D011506:Proteins / Q000378:metabolism*
C507066:Amtn protein, mouse; D003746:Dental Enamel Proteins; C465579:FDC secreted protein, mouse; D047908:Intracellular Signaling Peptides and Proteins; C555400:ODAM protein, mouse; D011506:Proteins
Aggregatibacter actinomycetemcomitans; Amelotin; FDC-SP; Inflammation; Junctional epithelium; ODAM; Porphyromonas gingivalis