Minimally Invasive Liver Preconditioning for Hepatocyte Transplantation in Rats.
In the context of cell transplantation in the liver parenchyma, preconditioning is essential to enhance cell engraftment and liver repopulation. The authors have developed a minimally invasive technique of temporary portal embolization using an absorbable material, called reversible portal vein embolization. We hereby describe the method for isolating hepatocytes from a donor rat before transplanting hepatocytes after reversible portal vein embolization in the recipient.
INSERM U1193, Paul Brousse Hospital, Villejuif, F-91807, France.|Department of Digestive Minimally Invasive Surgery, Antoine Béclère Hospital, AP-HP, Clamart, F-92140, France.|Faculté de Médecine Paris-Sud, Paris-Saclay University, Orsay, F-91405, France.
Martin
M
Gaillard
INSERM U1193, Paul Brousse Hospital, Villejuif, F-91807, France. ibrahim.dagher@abc.aphp.fr.|Department of Digestive Minimally Invasive Surgery, Antoine Béclère Hospital, AP-HP, Clamart, F-92140, France. ibrahim.dagher@abc.aphp.fr.|Faculté de Médecine Paris-Sud, Paris-Saclay University, Orsay, F-91405, France. ibrahim.dagher@abc.aphp.fr.
Ibrahim
I
Dagher
D000818:Animals; D002469:Cell Separation / Q000295:instrumentation / Q000379:methods; D017690:Cell Transplantation / Q000295:instrumentation / Q000379:methods*; D005780:Gelatin / Q000008:administration & dosage; D022781:Hepatocytes / Q000637:transplantation*; D008099:Liver / Q000098:blood supply / Q000166:cytology / Q000502:physiology* / Q000601:surgery; D008115:Liver Regeneration; D008297:Male; D019060:Minimally Invasive Surgical Procedures / Q000379:methods; D010477:Perfusion / Q000295:instrumentation / Q000379:methods; D011169:Portal Vein / Q000601:surgery*; D051381:Rats; D013154:Spleen / Q000601:surgery; D019172:Transplantation Conditioning / Q000379:methods*
D005780:Gelatin
Hepatocyte isolation; Hepatocyte transplantation; Liver perfusion; Liver preconditioning; Portal vein embolization