Novel biogenic synthesis of silver nanoparticles and their therapeutic potential.
Here, we explored the medicinal uses of the novel biogenic silver nanoparticles ofPterospermum acerifolium(PaAgNPs) as a cost effective, eco-friendly, reducing and stabilizing compounds. The formation of PaAgNPs was confirmed by changing its color from colorless to yellowish brown, with maximum absorbance at 417 nm. FTIR spectrum of PaAgNPs suggested the presence of polycyclic compound similar to betulinic acid which plays as a capping agent and provided stability to PaAgNPs. FESEM and HRTEM images depicted the spherical shape of synthesized biogenic silver nanoparticles with an average particle size range of 10-20 nm. The EDX spectrum of the solution confirmed the presence of elemental silver signals. The crystalline nature of PaAgNPs was identified by XRD technique and its stability was recorded using Zeta potential analyzer. The antioxidant potential was assayed using diphenyl-beta-picrylhydrazyl (DPPH). Maximum free radical scavenging action of PaAgNPs was 69.52% as compared to 63.53% for PALE. Using a model of carrageenan-induced paw edema in rats, PaAgNPs showed two-fold enhanced anti-inflammatory activityin vivo.
Molecular Microbiology Laboratory, Biotechnology Department, Indian Institute of Technology, Roorkee 247667, Uttarakhand, India.
Navdeep
N
Raghuwanshi
Sapience Bioanalytical Research Lab, Bhopal 462021, Madhya Pradesh, India.
Ashish
A
Pathak
Molecular Microbiology Laboratory, Biotechnology Department, Indian Institute of Technology, Roorkee 247667, Uttarakhand, India.
Alok
A
Patel
Molecular Microbiology Laboratory, Biotechnology Department, Indian Institute of Technology, Roorkee 247667, Uttarakhand, India.
Priya
P
Vashisth
Molecular Microbiology Laboratory, Biotechnology Department, Indian Institute of Technology, Roorkee 247667, Uttarakhand, India.
Harmeet
H
Singh
Sapience Bioanalytical Research Lab, Bhopal 462021, Madhya Pradesh, India.
Amit Kumar
AK
Srivastava
Molecular Microbiology Laboratory, Biotechnology Department, Indian Institute of Technology, Roorkee 247667, Uttarakhand, India,, vikasfbs@gmail.com.
Vikas
V
Pruthi
D000818:Animals; D000893:Anti-Inflammatory Agents / Q000627:therapeutic use*; D000975:Antioxidants / Q000627:therapeutic use*; D005260:Female; D008297:Male; D019660:Malvaceae / Q000378:metabolism*; D053768:Metal Nanoparticles / Q000737:chemistry / Q000627:therapeutic use*; D010936:Plant Extracts / Q000737:chemistry; D051381:Rats; D017208:Rats, Wistar; D012834:Silver / Q000737:chemistry / Q000378:metabolism; D017550:Spectroscopy, Fourier Transform Infrared
D000893:Anti-Inflammatory Agents; D000975:Antioxidants; D010936:Plant Extracts; D012834:Silver