Inbred mice strain shows neurobehavioral changes when exposed to tannery effluent.
The bovine leather processing (tanning industries) stands as a generating activity of potentially toxic waste. The emission of untreated effluents into the environment may cause serious harm to human and environmental health. Nevertheless, few studies have investigated the possible effects of intake of these effluents in experimental mammalian models. Thus, this study aimed to evaluate the neurobehavioral effects of chronic intake of different tannery effluent concentrations diluted with water (0.1, 1, and 5%) in male C57BL/6J mice. After 120 days of exposure, the animals were subjected to different behavioral tests, predictive of anxiety (elevated plus maze (EPM), open-field (OF), and neophobia test), depression (forced swim), and memory deficits (object recognition test). From the EPM test, it was observed that the mice exposed to 0.1, 1, and 5% of tannery effluents showed higher anxiety scores compared to the animals in the control group. However, the results of this study revealed no differences among the experimental groups in the proportion (percentage) of locomotion in the central quarters/total locomotion calculated (by OF), considered an indirect measure for anxiety. At neophobia test, all the animals exposed to chronic intake of tannery effluents showed higher latency time to start eating, which corresponds to an anxiogenic behavior. Regarding the forced swim test, it was observed that the animals exposed to tannery effluents had longer time in immobility behavior, suggesting a predictive behavior to depression. Finally, the object recognition test showed that the treatments did not cause damage to the animals' memory. The recognition rate of the new object did not differ among the experimental groups. Thus, it is concluded that male C57BL/6J mice (inbred strain) exposed to tannery effluents have predictive neurobehavioral changes of anxiety and depression, without memory deficit.
Instituto Federal Goiano-Câmpus Urutaí, Rodovia Geraldo Silva Nascimento, 2.5 km, Zona Rural, Urutaí, GO, Brazil.
Joyce Moreira
JM
de Souza
Instituto Federal Goiano-Câmpus Urutaí, Rodovia Geraldo Silva Nascimento, 2.5 km, Zona Rural, Urutaí, GO, Brazil.
Wellington Alves Mizael
WA
da Silva
Instituto Federal Goiano-Câmpus Urutaí, Rodovia Geraldo Silva Nascimento, 2.5 km, Zona Rural, Urutaí, GO, Brazil.
Bruna
B
de Oliveira Mendes
Instituto Federal Goiano-Câmpus Urutaí, Rodovia Geraldo Silva Nascimento, 2.5 km, Zona Rural, Urutaí, GO, Brazil.
Abraão Tiago Batista
AT
Guimarães
Instituto Federal Goiano-Câmpus Urutaí, Rodovia Geraldo Silva Nascimento, 2.5 km, Zona Rural, Urutaí, GO, Brazil.
Aline Sueli
AS
de Lima Rodrigues
Instituto Federal Goiano-Câmpus Urutaí, Rodovia Geraldo Silva Nascimento, 2.5 km, Zona Rural, Urutaí, GO, Brazil.
Mateus Flores
MF
Montalvão
Instituto Federal Goiano-Câmpus Urutaí, Rodovia Geraldo Silva Nascimento, 2.5 km, Zona Rural, Urutaí, GO, Brazil.
Dieferson
D
da Costa Estrela
Instituto Federal Goiano-Câmpus Urutaí, Rodovia Geraldo Silva Nascimento, 2.5 km, Zona Rural, Urutaí, GO, Brazil.
Anderson Rodrigo
AR
da Silva
Instituto Federal Goiano-Câmpus Urutaí, Rodovia Geraldo Silva Nascimento, 2.5 km, Zona Rural, Urutaí, GO, Brazil. guilhermeifgoiano@gmail.com.
Guilherme
G
Malafaia
D000818:Animals; D001007:Anxiety / Q000139:chemically induced; D001522:Behavior, Animal / Q000187:drug effects*; D002417:Cattle; D003863:Depression / Q000139:chemically induced; D007220:Industrial Waste; D008297:Male; D008568:Memory / Q000187:drug effects; D051379:Mice; D008810:Mice, Inbred C57BL; D013633:Tanning; D014874:Water Pollutants, Chemical / Q000633:toxicity*
D007220:Industrial Waste; D014874:Water Pollutants, Chemical
Animal model; Anxiety; Industrial waste; Toxicity; Xenobiotics