Effects of 4-nonylphenol on the steroidogenesis of human adrenocarcinoma cell line (NCI-H295R).
In this study, the human H295R adrenocarcinoma cell line was exposed to different concentrations (0.04, 0.2, 1.0, 2.5 or 5 µg/mL) of nonylphenol (NP) to investigate its impact on the inhibition or induction of the steroid hormones production during 48 h of in vitro culture. The hormone production was measured using ELISA kits. Results of this in vitro study suggest various effect of nonylphenol in relatively low concentrations on the selected steroid hormones production by the human H295R adrenocarcinoma cell line. The inhibiting impact on progesterone and androstenedione production was observed. The amount of progesterone was significantly decreased at 1.0, 2.5 and 5 µg/mL NP. Equally, the androstenedione production significantly decreased at 5 µg/mL NP. On the other hand, the amount of testosterone and 17β-estradiol was induced after nonylphenol exposition. The significant increase of testosterone level was found out at treatment with 5 µg/mL NP. 17β-estradiol production significantly increased at the doses of 2.5 and 5 µg/mL NP.
a Department of Animal Physiology , Faculty of Biotechnology and Food Sciences, Slovak University of Agriculture , Nitra , Slovak Republic.
Jana
J
Bistakova
b National Institute of Chemical Safety , Budapest , Hungary.
Zsolt
Z
Forgacs
b National Institute of Chemical Safety , Budapest , Hungary.
Zsuzsa
Z
Bartos
b National Institute of Chemical Safety , Budapest , Hungary.
Maria Racz
MR
Szivosne
a Department of Animal Physiology , Faculty of Biotechnology and Food Sciences, Slovak University of Agriculture , Nitra , Slovak Republic.
Tomas
T
Jambor
c AgroBioTech Research Centre, Slovak University of Agriculture , Nitra , Slovak Republic.
Zuzana
Z
Knazicka
a Department of Animal Physiology , Faculty of Biotechnology and Food Sciences, Slovak University of Agriculture , Nitra , Slovak Republic.
Eva
E
Tvrda
d St. Ladislav Institute of Health and Social Sciences, Nove Zamky, St. Elizabeth University of Health and Social Sciences , Bratislava , Slovak Republic.
Lubica
L
Libova
e Institute of Biology, Faculty of Geography and Biology, Pedagogical University of Cracow , Cracow , Poland.
Zofia
Z
Goc
a Department of Animal Physiology , Faculty of Biotechnology and Food Sciences, Slovak University of Agriculture , Nitra , Slovak Republic.|e Institute of Biology, Faculty of Geography and Biology, Pedagogical University of Cracow , Cracow , Poland.
Peter
P
Massanyi
a Department of Animal Physiology , Faculty of Biotechnology and Food Sciences, Slovak University of Agriculture , Nitra , Slovak Republic.
Norbert
N
Lukac
D000306:Adrenal Cortex Neoplasms / Q000378:metabolism; D045744:Cell Line, Tumor / Q000187:drug effects* / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D052244:Endocrine Disruptors / Q000494:pharmacology*; D004797:Enzyme-Linked Immunosorbent Assay; D004958:Estradiol / Q000096:biosynthesis; D006801:Humans; D010636:Phenols / Q000494:pharmacology*; D011374:Progesterone / Q000096:biosynthesis; D013256:Steroids / Q000096:biosynthesis; D013739:Testosterone / Q000096:biosynthesis; D018675:Toxicity Tests
D052244:Endocrine Disruptors; D010636:Phenols; D013256:Steroids; D013739:Testosterone; D011374:Progesterone; D004958:Estradiol; C041594:4-nonylphenol
17β-estradiol; H295R cell line; androstenedione; nonylphenol; progesterone; testosterone