The Complete Mitochondrial Genome of Aleurocanthus camelliae: Insights into Gene Arrangement and Genome Organization within the Family Aleyrodidae.
There are numerous gene rearrangements and transfer RNA gene absences existing in mitochondrial (mt) genomes of Aleyrodidae species. To understand how mt genomes evolved in the family Aleyrodidae, we have sequenced the complete mt genome ofAleurocanthus camelliaeand comparatively analyzed all reported whitefly mt genomes. The mt genome ofA. camelliaeis 15,188 bp long, and consists of 13 protein-coding genes, two rRNA genes, 21 tRNA genes and a putative control region (GenBank: KU761949). The tRNA gene,trnI, has not been observed in this genome. The mt genome has a unique gene order and shares most gene boundaries withTetraleurodes acaciae. Nineteen of 21 tRNA genes have the conventional cloverleaf shaped secondary structure and two (trnS₁andtrnS₂) lack the dihydrouridine (DHU) arm. Using ARWEN and homologous sequence alignment, we have identified five tRNA genes and revised the annotation for three whitefly mt genomes. This result suggests that most absent genes exist in the genomes and have not been identified, due to be lack of technology and inference sequence. The phylogenetic relationships among 11 whiteflies andDrosophila melanogasterwere inferred by maximum likelihood and Bayesian inference methods.Aleurocanthus camelliaeandT. acaciaeform a sister group, and all threeBemisia tabaciand twoBemisia aferstrains gather together. These results are identical to the relationships inferred from gene order. We inferred that gene rearrangement plays an important role in the mt genome evolved from whiteflies.
Tea Research Institute, Chongqing Academy of Agricultural Science, Chongqing 402160, China. chensc0318@163.com.
Shi-Chun
SC
Chen
Tea Research Institute, Chongqing Academy of Agricultural Science, Chongqing 402160, China. wangxiaoqing2891@126.com.
Xiao-Qing
XQ
Wang
Tea Science Department, College of Horticulture, Sichuan Agricultural University, Ya'an 611130, China. lipinwu@126.com.
Pin-Wu
PW
Li
Tea Research Institute, Chongqing Academy of Agricultural Science, Chongqing 402160, China. huxiang2016@126.com.
Xiang
X
Hu
Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing 400715, China. wangjinjun@swu.edu.cn.
Jin-Jun
JJ
Wang
Tea Research Institute, Chongqing Academy of Agricultural Science, Chongqing 402160, China. pptea2006@163.com.
Ping
P
Peng
D000818:Animals; D001483:Base Sequence; D001499:Bayes Theorem; D005075:Biological Evolution; D017353:Gene Deletion; D063990:Gene Ontology; D015321:Gene Rearrangement; D049750:Genome, Insect; D054629:Genome, Mitochondrial; D006430:Hemiptera / Q000145:classification / Q000235:genetics*; D059014:High-Throughput Nucleotide Sequencing; D058977:Molecular Sequence Annotation; D009690:Nucleic Acid Conformation; D016366:Open Reading Frames; D010802:Phylogeny; D012343:RNA, Transfer / Q000235:genetics; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA
D012343:RNA, Transfer
Aleurocanthus camelliae; genome evolution; mitochondrial genome; rearrangement; whitefly