Alterations in oviductal cilia morphology and reduced expression of axonemal dynein in diabetic NOD mice.
In the present study, we examined the morphology of cilia and expression of the dynein intermediate chain 2 (DNAI2) in the oviduct of non-obese diabetic (NOD) mice. Results obtained with immunohistochemistry showed that DNAI2 expression was reduced in oviducts of diabetic NOD (dNOD) mice, as compared to that observed in the normoglycemic NOD (cNOD) group, especially in the acyclic dNOD mice. Oviductal cilia of dNOD mice appeared to be reduced in number. Results obtained with Western blot analysis revealed that the expression of DNAI2 protein was significantly less in oviducts of dNOD mice as compared to that of cNOD mice corroborating the results obtained with immunohistochemistry. Electron microscopic examination and quantitative imaging of thin sections of Epon-embedded oviducts of both dNOD and cNOD mice confirmed the reduction of the number of cilia in the oviduct of the dNOD group which also displayed aberrant axonemal ultrastructure, including disorganization of the axoneme and alteration of microtubule doublets into singlets as well as disruption of the plasma membrane in many cilia. Taken together, the present findings suggest that structural alterations of oviductal cilia in female diabetic NOD mice might be detrimental to the normal function of these particular cell structures in gamete transport.
Department of Biomedical and Molecular Sciences, Faculty of Health Sciences, Queen's University, Kingston, Ontario, K7M 6X1, Canada.
Stephanie L
SL
Shorter
Department of Biomedical and Molecular Sciences, Faculty of Health Sciences, Queen's University, Kingston, Ontario, K7M 6X1, Canada.
Ahmad J H
AJ
Albaghdadi
Department of Biomedical and Molecular Sciences, Faculty of Health Sciences, Queen's University, Kingston, Ontario, K7M 6X1, Canada. Electronic address: kanfwk@queensu.ca.
Frederick W K
FW
Kan
D000818:Animals; D056444:Axonemal Dyneins / Q000096:biosynthesis*; D054468:Axoneme / Q000378:metabolism / Q000473:pathology / Q000648:ultrastructure; D002923:Cilia / Q000378:metabolism* / Q000473:pathology / Q000648:ultrastructure; D003920:Diabetes Mellitus / Q000235:genetics* / Q000473:pathology; D004195:Disease Models, Animal; D005187:Fallopian Tubes / Q000378:metabolism* / Q000473:pathology / Q000648:ultrastructure; D005260:Female; D006801:Humans; D051379:Mice; D016688:Mice, Inbred NOD / Q000235:genetics; D008854:Microscopy, Electron
C531409:Dnaic2 protein, mouse; D056444:Axonemal Dyneins
Axonemal dynein; Female NOD mice; Oviductal cilia; Type 1 diabetes