Structural Prediction andIn SilicoPhysicochemical Characterization for Mouse Caltrin I and Bovine Caltrin Proteins.
It is known that caltrin (calcium transport inhibitor) protein binds to sperm cells during ejaculation and inhibits extracellular Ca2+uptake. Although the sequence and some biological features of mouse caltrin I and bovine caltrin are known, their physicochemical properties and tertiary structure are mainly unknown. We predicted the 3D structures of mouse caltrin I and bovine caltrin by molecular homology modeling and threading. Surface electrostatic potentials and electric fields were calculated using the Poisson-Boltzmann equation. Several different bioinformatics tools and available web servers were used to thoroughly analyze the physicochemical characteristics of both proteins, such as their Kyte and Doolittle hydropathy scores and helical wheel projections. The results presented in this work significantly aid further understanding of the molecular mechanisms of caltrin proteins modulating physiological processes associated with fertilization.
Instituto de Investigaciones Biológicas y Tecnológicas (IIByT) CONICET-Universidad Nacional de Córdoba, and Instituto de Ciencia y Tecnología de los Alimentos (ICTA), Cátedra de Química Biológica, Departamento de Química Industrial y Aplicada, Facultad de Ciencias Exactas, Físicas y Naturales, Córdoba, Argentina.
Ernesto J
EJ
Grasso
Instituto de Investigaciones Biológicas y Tecnológicas (IIByT) CONICET-Universidad Nacional de Córdoba, and Instituto de Ciencia y Tecnología de los Alimentos (ICTA), Cátedra de Química Biológica, Departamento de Química Industrial y Aplicada, Facultad de Ciencias Exactas, Físicas y Naturales, Córdoba, Argentina.
Adolfo E
AE
Sottile
Instituto de Investigaciones Biológicas y Tecnológicas (IIByT) CONICET-Universidad Nacional de Córdoba, and Instituto de Ciencia y Tecnología de los Alimentos (ICTA), Cátedra de Química Biológica, Departamento de Química Industrial y Aplicada, Facultad de Ciencias Exactas, Físicas y Naturales, Córdoba, Argentina.
Carlos E
CE
Coronel
bovine caltrin; electrostatic surface potentials; homology modeling; hydropathy plots; mouse caltrin I; threading