Glucose transporter 8 immunoreactivity in astrocytic and microglial cells in subependymal areas of human brains.
Glucose transporter 8 (GLUT8), a glucose/fructose transporter, has been shown to be expressed in neuronal cells in several brain areas. A recent immunohistochemical study has shown the presence of GLUT8 in the cytoplasm of epithelial cells of the choroid plexus and ependymal cells. In this study, localization of GLUT8 in glial cells was investigated using immunohistochemical methods. Immunoreactivity for GLUT8 was observed in cells showing astrocytic or microglial structural features located around the lateral ventricles. Confocal microscopic examination revealed that subependymal GLUT8-positive cells with large amounts of cytoplasm mainly show clear immunoreactivity for vimentin, while they were also colocalized with weak immunoreactivity for glial fibrillary acidic protein (GFAP) within the cytoplasm of some cells. In addition, some GLUT8-positive cells with small amounts of cytoplasm and small nuclei showed CD68 or HLA-DR immunoreactivity, indicating them to be cells of microglia/macrophage lineage. These findings suggest that glucose/fructose is transported into the cytoplasm of vimentin- or GFAP-positive astrocytic and CD68- or HLA-DR-positive microglial cells located around the lateral ventricle.
Department of Pathology and Host Defense, Faculty of Medicine, Kagawa University, Kagawa 761-0793, Japan; Department of Perinatology and Gynecology, Faculty of Medicine, Kagawa University, Kagawa 761-0793, Japan.
Masato
M
Mashima
Department of Pathology and Host Defense, Faculty of Medicine, Kagawa University, Kagawa 761-0793, Japan.
Yoichi
Y
Chiba
Department of Pathology and Host Defense, Faculty of Medicine, Kagawa University, Kagawa 761-0793, Japan.
Ryuta
R
Murakami
Department of Pathology and Host Defense, Faculty of Medicine, Kagawa University, Kagawa 761-0793, Japan; Department of Anesthesiology, Faculty of Medicine, Kagawa University, Kagawa 761-0793, Japan.
Naoya
N
Uemura
Department of Pathology and Host Defense, Faculty of Medicine, Kagawa University, Kagawa 761-0793, Japan.
Koichi
K
Matsumoto
Department of Pathology and Host Defense, Faculty of Medicine, Kagawa University, Kagawa 761-0793, Japan.
Machi
M
Kawauchi
Department of Perinatology and Gynecology, Faculty of Medicine, Kagawa University, Kagawa 761-0793, Japan.
Kenji
K
Kanenishi
Department of Perinatology and Gynecology, Faculty of Medicine, Kagawa University, Kagawa 761-0793, Japan.
Toshiyuki
T
Hata
Department of Pathology and Host Defense, Faculty of Medicine, Kagawa University, Kagawa 761-0793, Japan. Electronic address: masaueno@med.kagawa-u.ac.jp.
Masaki
M
Ueno
D015703:Antigens, CD / Q000378:metabolism; D015214:Antigens, Differentiation, Myelomonocytic / Q000378:metabolism; D001253:Astrocytes / Q000378:metabolism*; D001921:Brain / Q000166:cytology / Q000378:metabolism*; D004805:Ependyma / Q000166:cytology / Q000378:metabolism; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D051246:Glucose Transport Proteins, Facilitative / Q000378:metabolism*; D006684:HLA-DR Antigens / Q000378:metabolism; D006801:Humans; D017628:Microglia / Q000378:metabolism*; D000097382:CD68 Molecule
D015703:Antigens, CD; D015214:Antigens, Differentiation, Myelomonocytic; D005904:Glial Fibrillary Acidic Protein; D051246:Glucose Transport Proteins, Facilitative; D006684:HLA-DR Antigens; C067980:CD68 antigen, human; D000097382:CD68 Molecule; C405325:SLC2A8 protein, human
Astrocyte; Fructose; GLUT8; Vimentin