Enzymatic Precipitation Enhanced Surface Plasmon Resonance Immunosensor for the Detection of Salmonella in Powdered Milk.
Contamination of food by pathogenic bacteria has always been a serious threat for human health. The amount of food exports and imports has been increasing in recent years which requires precise food quality control with short analysis time and simplified sample treatment. Surface plasmon resonance (SPR) immunosensor enhanced by biocatalyzed precipitation was developed for the analysis of Salmonella in dairy products. The specific capture antibody was immobilized on the SPR chip which allowed a direct label-free detection of Salmonella Typhimurium with the limit of detection (LOD) of 104CFU·mL-1and the analysis time of 10 min. Alternatively, the secondary detection antibody was conjugated with horseradish peroxidase to provide a signal enhancement by the biocatalyzed conversion of 4-chloro-1-naphthol to insoluble benzo-4-chlorocyclohexadienone. The formation of precipitate was studied in detail by atomic force microscopy (AFM). The sensitivity was increased 40 times in case of the precipitation-enhanced detection compared to the label-free approach. The optimized method provided LOD of 100 CFU·mL-1with linear range up to 106CFU·mL-1. The total time of analysis including bacteria binding and enhancement step was below 60 min. The capability to analyze real samples with complex matrices was demonstrated on the detection of Salmonella in powdered milk. The developed sensor represents simple and robust approach for routine monitoring of food contamination.
CEITEC MU and ‡Department of Biochemistry, Faculty of Science, Masaryk University , Kamenice 5, 625 00 Brno, Czech Republic.
Zdeněk
Z
Farka
CEITEC MU and ‡Department of Biochemistry, Faculty of Science, Masaryk University , Kamenice 5, 625 00 Brno, Czech Republic.
Tomáš
T
Juřík
CEITEC MU and ‡Department of Biochemistry, Faculty of Science, Masaryk University , Kamenice 5, 625 00 Brno, Czech Republic.
Matěj
M
Pastucha
CEITEC MU and ‡Department of Biochemistry, Faculty of Science, Masaryk University , Kamenice 5, 625 00 Brno, Czech Republic.
Petr
P
Skládal
D000818:Animals; D005506:Food Contamination / Q000032:analysis*; D007118:Immunoassay; D018625:Microscopy, Atomic Force; D008892:Milk / Q000382:microbiology*; D011208:Powders / Q000737:chemistry; D012486:Salmonella typhimurium / Q000302:isolation & purification*; D020349:Surface Plasmon Resonance
D011208:Powders