Development of magnetic molecularly imprinted polymers with double templates for the rapid and selective determination of amphenicol antibiotics in water, blood, and egg samples.
A magnetic mesoporous dual-template molecularly imprinted polymer (Fe3O4@mSiO2@DMIP) with a specific recognition capability for chloramphenicol (CAP) and florfenicol (FF) was synthesised. CAP and FF were used as dual-template molecules, α-methacrylic acid and Fe3O4@mSiO2@-CHCH2as dual functional monomers, and ethylene glycol dimethyl methacrylate as a crosslinking agent. For comparison, a magnetic mesoporous non-molecularly imprinted polymer (Fe3O4@mSiO2@NIP) was also prepared using the same synthesis procedure, but without the dual templates. The prepared polymers were characterised using scanning electron microscopy, Fourier-transform infrared spectroscopy and adsorption experiments. Results indicated that both the Fe3O4@mSiO2@DMIP and the Fe3O4@mSiO2@NIP were microspherical nanoparticles, and the surface of the Fe3O4@mSiO2@DMIP was rougher than that of the Fe3O4@mSiO2@NIP. In addition, the prepared Fe3O4@mSiO2@DMIP possessed a higher adsorption capacity and better selectivity for CAP and FF than the Fe3O4@mSiO2@NIP. The maximum static adsorption capacities of the Fe3O4@mSiO2@ DMIP for CAP and FF were 146.5 and 190.1mgg-1, respectively, whereas those of the Fe3O4@mSiO2@NIP were 50.0 and 44.0mgg-1, respectively. The obtained Fe3O4@mSiO2@DMIP particles were applied as a magnetic solid-phase extraction sorbent for the rapid and selective extraction of CAP, FF, and thiamphenicol (TAP) in water, chicken blood and egg samples. The method of magnetic molecularly imprinted solid-phase extraction (M-MISPE) coupled to high-performance liquid chromatography with UV detection (HPLC-UV) was conducted to detect CAP, FF, and TAP. The limits of detection for CAP, FF, and TAP were 0.16, 0.08, and 0.08μgkg-1, respectively. The average recovery and precision values for the spiked water, chicken blood, and egg samples ranged from 88.3% to 99.1% and 2.7% to 7.9%, respectively. Given its rapidity, selectivity, and sensitivity, the developed method of M-MISPE coupled to HPLC-UV detection has good application prospects in environmental, biological, and food samples.
College of Chemistry and Chemical Engineering, Zhaoqing University, Duanzhou District, Zhaoqing, Guangdong, 526061, China.
Shoulian
S
Wei
Department of Chinese Medicine and Biology, Guangdong Food and Drug Vocational College, Guangzhou 510520, China.
Jianwen
J
Li
College of Chemistry and Chemical Engineering, Zhaoqing University, Duanzhou District, Zhaoqing, Guangdong, 526061, China.
Yong
Y
Liu
College of Chemistry and Chemical Engineering, Zhaoqing University, Duanzhou District, Zhaoqing, Guangdong, 526061, China. Electronic address: jinkuima@gmail.com.
Jinkui
J
Ma
D000327:Adsorption; D000818:Animals; D000900:Anti-Bacterial Agents / Q000032:analysis* / Q000097:blood*; D002623:Chemistry Techniques, Analytical / Q000379:methods*; D002645:Chickens; D002851:Chromatography, High Pressure Liquid; D004531:Eggs / Q000032:analysis*; D008280:Magnetics; D008689:Methacrylates / Q000737:chemistry; D008855:Microscopy, Electron, Scanning; D054802:Molecular Imprinting; D053758:Nanoparticles / Q000032:analysis / Q000648:ultrastructure; D011108:Polymers / Q000737:chemistry*; D012680:Sensitivity and Specificity; D052616:Solid Phase Extraction; D014867:Water / Q000737:chemistry*
D000900:Anti-Bacterial Agents; D008689:Methacrylates; D011108:Polymers; D014867:Water; C008384:methacrylic acid
Amphenicol antibiotic; Chicken blood; Dual-template; Eggs; Magnetic solid phase extraction; Molecular imprinted polymer