Studies on the regulation of lipid metabolism and its mechanism of the iridoids rich fraction in Valeriana jatamansi Jones.
Valeriana jatamansi Jones, a plant with heart-shaped leaves in the Valeriana genus of Valerianaceae, is widely used in Chinese folk medicine. Iridoid is an important constituent of V. jatamansi that contributes to the pharmacological efficacy of the herb. This study aims to investigate the regulation of lipid metabolism and its mechanism of the iridoids rich fraction in V. jatamansi (IRFV). A high fat diet was used to establish the hyperlipidemia rat model, with 2mg/kg/d of simvastatin as a positive control, fed with 7.5, 15, and 30mg/kg/d of IRFV for 20days to investigate the lipid regulation activity and mechanism of IRFV. Body weight, liver index, total cholesterol (TC), triglyceride (TG), low-density lipoprotein cholesterol (LDL-C), and high-density lipoprotein cholesterol (HDL-C) in both serum and liver, as well as total bile acid (TBA), aspartate aminotransferase (AST), and alanine aminotransferase (ALT) in serum were measured. The lipoprotein lipase (LPL) and hepatic lipase (HL) activities and the apoprotein A5 (ApoA5), peroxisome proliferator-activated receptor α (PPAR-α), sterol regulatory element-binding proteins (SREBP-1c), and liver X receptor α (LXR-α) protein expressions were observed. Liver pathology was described through hematoxylin-eosin (HE) staining. Compared with the model group, three different IRFV dosages can slow down the weight gain of rats, reduce the contents of TG, and increase the contents of HDL-C in serum. Low IRFV dosage can significantly reduce the AST and ALT contents in serum, liver index, and the TG contents in liver, enhance LPL activity. Medium IRFV dosage can significantly decrease the TG and LDL-C contents in liver. High IRFV dosage can significantly reduce LDL-C, TBA, AST, and ALT contents in serum, and enhance HL activity. Three different IRFV dosages can significantly increase the ApoA5 and PPAR-α protein expression and decrease the SREBP-1c protein expression. Furthermore, the LXR-α protein expression decreased in low- and high-dose groups. Liver tissue pathological observation showed that IRFV can improve cell degeneration to a certain extent. These results strongly suggest that IRFV play significant roles in regulating lipid metabolism, the mechanism may be related to the increased ApoA5 protein expression.
School of Life Science and Engineering, Southwest Jiaotong University, Chengdu 610031, PR China.
Jiali
J
Zhu
School of Life Science and Engineering, Southwest Jiaotong University, Chengdu 610031, PR China.
Keke
K
Xu
School of Life Science and Engineering, Southwest Jiaotong University, Chengdu 610031, PR China.
Xuemei
X
Zhang
School of Life Science and Engineering, Southwest Jiaotong University, Chengdu 610031, PR China.
Jiahong
J
Cao
School of Life Science and Engineering, Southwest Jiaotong University, Chengdu 610031, PR China.
Zhanrong
Z
Jia
School of Life Science and Engineering, Southwest Jiaotong University, Chengdu 610031, PR China.
Ruocong
R
Yang
School of Life Science and Engineering, Southwest Jiaotong University, Chengdu 610031, PR China.
Chaoying
C
Ma
Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, PR China.
Chang
C
Chen
School of Basic Medicine, Chengdu University of Traditional Chinese Medicine,Chengdu 611137, PR China. Electronic address: zhte2003@aliyun.com.
Tiane
T
Zhang
School of Life Science and Engineering, Southwest Jiaotong University, Chengdu 610031, PR China. Electronic address: yzhiy@swjtu.edu.cn.
Zhiyong
Z
Yan
D000818:Animals; D005260:Female; D039823:Iridoids / Q000302:isolation & purification / Q000494:pharmacology*; D050356:Lipid Metabolism / Q000187:drug effects* / Q000502:physiology*; D008099:Liver / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D008297:Male; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology*; D018517:Plant Roots; D051381:Rats; D017207:Rats, Sprague-Dawley; D027343:Rhizome; D014632:Valerian
D039823:Iridoids; D010936:Plant Extracts
Hyperlipidemia; Iridoids rich fraction; Lipid metabolism; Mechanism; Regulation; Valeriana jatamansi Jones