pH-degradable PVA-based nanogels via photo-crosslinking of thermo-preinduced nanoaggregates for controlled drug delivery.
pH-Degradable PVA nanogels, which are prepared by photo-crosslinking thermo-preinduced PVA nanoaggregates in water without any surfactants or toxic organic solvents, are used for intracellular PTX release and anticancer treatment. These nanogels fast degraded at mildly acidic conditions with a pH-triggered PTX release, and the degradation products are only native PVA and poly(hydroxyethyl acrylate) (PHEA) as well as acetaldehyde without any toxic byproducts. The nanogel sizes could be tailored by different temperatures during the crosslinking process. The results of confocal microscopy and flow cytometry revealed that smaller nanogels exhibited enhanced internalization with MCF-7 cells than the ones treated with larger nanogels, by which the smaller PTX-loaded nanogels induced a more significant cytotoxicity against MCF-7 cells. GRAPHIC ABSTRACT: pH-Degradable PVA nanogels can be prepared by photo-crosslinking of thermo-preinduced nanoaggregates with tailored nanogel sizes given their pH-triggered PTX release and fast acid-degradation into native PVA and cell-compatible poly(hydroxyethyl acrylate) (PHEA) as well as acetaldehyde.
Institute of Chemistry and Biochemistry, Freie Universität Berlin, Takustrasse 3, Berlin 14195, Germany. Electronic address: chenwei1984@zedat.fu-berlin.de.
Wei
W
Chen
Institute of Chemistry and Biochemistry, Freie Universität Berlin, Takustrasse 3, Berlin 14195, Germany.
Yong
Y
Hou
Institute of Chemistry and Biochemistry, Freie Universität Berlin, Takustrasse 3, Berlin 14195, Germany.
Zhaoxu
Z
Tu
Institute of Chemistry and Biochemistry, Freie Universität Berlin, Takustrasse 3, Berlin 14195, Germany.
Lingyan
L
Gao
Institute of Chemistry and Biochemistry, Freie Universität Berlin, Takustrasse 3, Berlin 14195, Germany. Electronic address: haag@chemie.fu-berlin.de.
Rainer
R
Haag
D000072283:A549 Cells; D000972:Antineoplastic Agents, Phytogenic / Q000008:administration & dosage / Q000737:chemistry; D001557:Benzenesulfonates / Q000737:chemistry / Q000528:radiation effects; D002470:Cell Survival / Q000187:drug effects; D003692:Delayed-Action Preparations; D016503:Drug Delivery Systems; D065546:Drug Liberation; D016650:Fluorescein-5-isothiocyanate / Q000008:administration & dosage / Q000737:chemistry; D005456:Fluorescent Dyes / Q000008:administration & dosage / Q000737:chemistry; D005782:Gels; D006801:Humans; D006863:Hydrogen-Ion Concentration; D061986:MCF-7 Cells; D049329:Nanostructures / Q000008:administration & dosage / Q000737:chemistry / Q000528:radiation effects; D017239:Paclitaxel / Q000008:administration & dosage / Q000737:chemistry; D011109:Polymethacrylic Acids / Q000008:administration & dosage / Q000737:chemistry / Q000528:radiation effects; D011142:Polyvinyl Alcohol / Q000008:administration & dosage / Q000737:chemistry / Q000528:radiation effects; D011407:Propane / Q000031:analogs & derivatives / Q000737:chemistry / Q000528:radiation effects; D014466:Ultraviolet Rays
C499598:2-hydroxy-1-(4-(hydroxyethoxy)phenyl)-2-methyl-1-propanone; D000972:Antineoplastic Agents, Phytogenic; D001557:Benzenesulfonates; D003692:Delayed-Action Preparations; D005456:Fluorescent Dyes; D005782:Gels; D011109:Polymethacrylic Acids; D011142:Polyvinyl Alcohol; D016650:Fluorescein-5-isothiocyanate; D017239:Paclitaxel; C029501:4-toluenesulfonic acid; D011407:Propane
Antitumor treatment; Drug delivery; PVA nanogel; Photo-crosslinking; pH-degradation