Sugar Composition Analysis of Fuzi Polysaccharides by HPLC-MSnand Their Protective Effects on Schwann Cells Exposed to High Glucose.
Fuzi has been used to treat diabetic complications for many years in china. In a previous study, we have shown that Fuzi aqueous extract can attenuate Diabetic peripheral neuropathy (DPN) in rats and protect Schwann cells from injury. Thus, the protective effect of Fuzi polysaccharides (FPS) on high glucose-induced SCs and the preliminary mechanism were investigated. Firstly, the FPS were obtained and their monose composition was analyzed by the combination of pre-column derivatization and high performance liquid chromatography coupled with electrospray ionization multi-tandem mass spectrometry (HPLC/ESI-MSn). The results witnessed the efficiency of this method and seven monosaccharides were tentatively identified, among which fucose was first reported. Simultaneously,m/z215 can be considered as diagnostic ions to confirm the number of monosaccharides. Next, high glucose-induced SC model was applied and divided into model group, treated group of FPS, normal and osmotic control group. After treatment for 48 h, the data showed FPS could significantly decrease the intracellular ROS and apoptosis, which were determined by the corresponding fluorescent probes. Then, the expression of oxidative stress-related proteins in SCs were measured by Western blot. Furthermore, the protein tests found that FPS markedly up-regulated superoxide dismutase (SOD), catalase (CAT) and peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC-1α) protein level, but down-regulated NADPH oxidase-1 (Nox1) protein level. Moreover, FPS could also increase AMP-activated protein kinase (AMPK) activation significantly. Hence, we preliminary deduced that AMPK-PGC-1α pathway may play an important role in the protective effect of FPS against high glucose-induced cell damage.
School of Chinese Materia Medica, Beijing University of Chinese Medicine, Wangjing Zhonghuan Road No. 6 School Range, Chaoyang District, Beijing 100102, China. wbb20131681@163.com.
Bei-Bei
BB
Wang
School of Chinese Materia Medica, Beijing University of Chinese Medicine, Wangjing Zhonghuan Road No. 6 School Range, Chaoyang District, Beijing 100102, China. wangjl120@163.com.
Jia-Li
JL
Wang
School of Chinese Materia Medica, Beijing University of Chinese Medicine, Wangjing Zhonghuan Road No. 6 School Range, Chaoyang District, Beijing 100102, China. Bucmyj2015@163.com.
Jiang
J
Yuan
School of Chinese Materia Medica, Beijing University of Chinese Medicine, Wangjing Zhonghuan Road No. 6 School Range, Chaoyang District, Beijing 100102, China. 18810689952@163.com.
Qing-Hua
QH
Quan
School of Chinese Materia Medica, Beijing University of Chinese Medicine, Wangjing Zhonghuan Road No. 6 School Range, Chaoyang District, Beijing 100102, China. 17801164351@163.com.
Rui-Fang
RF
Ji
School of Chinese Materia Medica, Beijing University of Chinese Medicine, Wangjing Zhonghuan Road No. 6 School Range, Chaoyang District, Beijing 100102, China. tanpengtcm@163.com.
Peng
P
Tan
Beijing Chinese Medicine Research Institute, Beijing University of Chinese Medicine, North Third Ring Road No. 11 School Range, Chaoyang District, Beijing 100029, China. hanjing8585@163.com.
Jing
J
Han
School of Chinese Materia Medica, Beijing University of Chinese Medicine, Wangjing Zhonghuan Road No. 6 School Range, Chaoyang District, Beijing 100102, China. liuyg0228@163.com.
Yong-Gang
YG
Liu
D000818:Animals; D002236:Carbohydrate Conformation; D002460:Cell Line; D002851:Chromatography, High Pressure Liquid; D003929:Diabetic Neuropathies / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D004224:Diterpenes; D004365:Drugs, Chinese Herbal; D005947:Glucose / Q000378:metabolism*; D013058:Mass Spectrometry; D018384:Oxidative Stress / Q000187:drug effects; D010088:Oxidoreductases / Q000096:biosynthesis; D000071248:Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha / Q000096:biosynthesis; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology; D011134:Polysaccharides / Q000737:chemistry / Q000494:pharmacology; D051381:Rats; D017382:Reactive Oxygen Species / Q000378:metabolism; D012583:Schwann Cells / Q000378:metabolism* / Q000473:pathology
D004224:Diterpenes; D004365:Drugs, Chinese Herbal; D000071248:Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha; D010936:Plant Extracts; D011134:Polysaccharides; C501429:Ppargc1a protein, rat; D017382:Reactive Oxygen Species; C575009:fuzi drug herbal; D010088:Oxidoreductases; D005947:Glucose
AMPK-PGC-1α pathway; Fuzi polysaccharides; HPLC-ESI-MSn; Schwann cells; antioxidant; monosaccharide composition