Effects of GSTP1 and GPX1 Polymorphisms on the Risk of Preeclampsia in Chinese Han Women.
OBJECTIVE
Increasing evidence shows that oxidative stress plays an important part in the pathophysiological mechanisms of preeclampsia (PE). Polymorphic variants of oxidative stress-related candidate genes GST1 and GPX1 can affect the antioxidant activities of their encoded enzymes. Therefore, this study aimed to explore the associational analysis between GSTP1 and GPX1 single nucleotide polymorphisms (SNPs) and susceptibility to PE in Chinese Han women.


METHODS
DNA from 1130 PE patients and 1226 healthy individuals was genotyped for SNPs rs1695 in GSTP1 and rs1050450 in GPX1 using a predesigned TaqMan SNP genotyping assay. The χ2 test compared differences in genetic distributions between the two groups in a case-control study.


RESULTS
No significant differences in allelic or genotypic frequencies of GSTP1 rs1695 or GPX1 rs1050450 were detected between cases and controls (GSTP1 rs1695: χ2=1.122, p=0.571 by genotype, χ2=0.138, p=0.710, odds ratio=1.027, 95% confidence interval 0.892-1.183 by allele; GPX1 rs1050450: χ2=0.036, p=0.982 by genotype, χ2=0.002, p=0.960, odds ratio=1.005, 95% confidence interval 0.822-1.229 by allele). Moreover, no significant differences in genetic distribution were found between early/late-onset PE or mild/severe PE and control subgroups.


CONCLUSIONS
Our results suggest that GSTP1 rs1695 and GPX1 rs1050450 SNPs have no effects on the risk of PE in the Chinese Han population. However, these results should be confirmed by replication in different populations.
Prenatal Diagnosis Center, the Affiliated Hospital of Qingdao University, Qingdao, China.
Huijie
H
Gao
Chao
C
Liu
Ping
P
Lin
Luo
L
Xu
Xiao
X
Li
Yujie
Y
Chen
Baohua
B
Cheng
Aiqin
A
Li
Shiguo
S
Liu
D000328:Adult; D000483:Alleles; D044466:Asian People; D016022:Case-Control Studies; D005260:Female; D015870:Gene Expression; D005787:Gene Frequency; D005838:Genotype; D005979:Glutathione Peroxidase / Q000235:genetics*; D051549:Glutathione S-Transferase pi / Q000235:genetics*; D006801:Humans; D018384:Oxidative Stress; D020641:Polymorphism, Single Nucleotide; D011225:Pre-Eclampsia / Q000175:diagnosis* / Q000208:ethnology / Q000235:genetics* / Q000473:pathology; D011247:Pregnancy; D012307:Risk Factors; D000094765:Glutathione Peroxidase GPX1
D005979:Glutathione Peroxidase; C496556:GSTP1 protein, human; D051549:Glutathione S-Transferase pi; D000094765:Glutathione Peroxidase GPX1; C000722067:GPX1 protein, human