A conserved inhibitory role of suppressor of cytokine signaling 1 (SOCS1) in salmon antiviral immunity.
The SOCS proteins are regulators of JAK/STAT signaling. A number of viral infections has been associated with SOCS upregulation. Here we report that SOCS1 mRNA expression is up-regulated in salmon alphavirus (SAV3) infected TO cells, while infectious pancreatic necrosis virus infection has a negligible effect. SAV3 infected salmon showed increased SOCS1 mRNA levels in heart correlating with increased viral transcripts. Together, the in vitro and in vivo data suggests that SAV3-induced SOCS1 expression may affect the outcome of the virus infection. Using CHSE-214 cells overexpressing SOCS1 we revealed increased SAV3 replication compared to control, suggesting that SOCS1 suppress the antiviral capacity of the cells. In IFNa1-treated cells, the suppression of viral replication was partially rescued by SOCS1 overexpression. The increased viral replication in SOCS1 transgene cells was likely a result of impaired IFN-signaling and the reduced expression of interferon-stimulated genes in the transgene cells underscores this.
The Norwegian College of Fishery Science, UiT The Arctic University of Norway, N-9037 Tromsø, Norway.
Mehrdad
M
Sobhkhez
The Norwegian College of Fishery Science, UiT The Arctic University of Norway, N-9037 Tromsø, Norway.
Lisbeth L
LL
Joensen
The Norwegian College of Fishery Science, UiT The Arctic University of Norway, N-9037 Tromsø, Norway.
Linn Greiner
LG
Tollersrud
The Norwegian College of Fishery Science, UiT The Arctic University of Norway, N-9037 Tromsø, Norway.
Guro
G
Strandskog
The Norwegian College of Fishery Science, UiT The Arctic University of Norway, N-9037 Tromsø, Norway.
Hanna L
HL
Thim
The Norwegian College of Fishery Science, UiT The Arctic University of Norway, N-9037 Tromsø, Norway. Electronic address: jorunn.jorgensen@uit.no.
Jorunn B
JB
Jørgensen
D000524:Alphavirus / Q000502:physiology*; D018354:Alphavirus Infections / Q000276:immunology*; D000818:Animals; D018175:Birnaviridae Infections / Q000276:immunology*; D002460:Cell Line; D005393:Fish Diseases / Q000276:immunology*; D029941:Fish Proteins / Q000235:genetics / Q000378:metabolism*; D005786:Gene Expression Regulation; D060226:Head Kidney / Q000473:pathology; D007113:Immunity, Innate; D018061:Infectious pancreatic necrosis virus / Q000502:physiology*; D016898:Interferon-alpha / Q000378:metabolism; D053612:Janus Kinases / Q000378:metabolism; D050791:STAT Transcription Factors / Q000378:metabolism; D012474:Salmon / Q000276:immunology*; D015398:Signal Transduction; D000071222:Suppressor of Cytokine Signaling 1 Protein / Q000235:genetics / Q000378:metabolism*; D019562:Viral Load
D029941:Fish Proteins; D016898:Interferon-alpha; D050791:STAT Transcription Factors; D000071222:Suppressor of Cytokine Signaling 1 Protein; D053612:Janus Kinases
Atlantic salmon; IPNV; Interferons; JAK/STAT signaling; SAV; SOCS