Using Flow Cytometry to Analyze Cryptococcus Infection of Macrophages.
Flow cytometry is a powerful analytical technique, which is increasingly being used to study the interaction between host cells and intracellular pathogens. Flow cytometry is capable of measuring a greater number of infected cells within a sample compared to alternative techniques such as fluorescence microscopy. This means that robust quantification of rare events during infection is possible. Our lab and others have developed flow cytometry methods to study interactions between host cells and intracellular pathogens, such as Cryptococcus neoformans, to quantify phagocytosis, intracellular replication, and non-lytic expulsion or "vomocytosis" from the phagosome. Herein we describe these methods and how they can be applied to the study of C. neoformans as well as other similar intracellular pathogens.
Institute of Microbiology and Infection, School of Biosciences, College of Life and Environmental Sciences, University of Birmingham, Birmingham, West Midlands, B15 2TT, UK.|Bateson Centre, Firth Court, University of Sheffield, Western Bank, Sheffield, South Yorkshire, UK.|Department of Infection, Immunity and Cardiovascular Disease, Medical School, University of Sheffield, Sheffield, South Yorkshire, UK.
Robert J
RJ
Evans
Institute of Microbiology and Infection, School of Biosciences, College of Life and Environmental Sciences, University of Birmingham, Birmingham, West Midlands, B15 2TT, UK.
Kerstin
K
Voelz
Bateson Centre, Firth Court, University of Sheffield, Western Bank, Sheffield, South Yorkshire, UK.|Department of Infection, Immunity and Cardiovascular Disease, Medical School, University of Sheffield, Sheffield, South Yorkshire, UK.
Simon A
SA
Johnston
Institute of Microbiology and Infection, School of Biosciences, College of Life and Environmental Sciences, University of Birmingham, Birmingham, West Midlands, B15 2TT, UK. R.C.May@bham.ac.uk.
Robin C
RC
May
D000818:Animals; D003453:Cryptococcosis / Q000382:microbiology*; D003455:Cryptococcus neoformans / Q000502:physiology*; D005434:Flow Cytometry / Q000379:methods*; D008264:Macrophages / Q000382:microbiology*; D051379:Mice; D013223:Statistics as Topic
Cryptococcus gattii; Cryptococcus neoformans; Flow cytometry; Infection; Macrophage; Mycology