Anvillea garcinii extract inhibits the oxidative burst of primary human neutrophils.
BACKGROUND
Anvillea garcinii Coss. & Durieu (Anv) plant is used as a traditional North African medicine against several diseases associated with inflammation. At inflammatory sites, reactive oxygen species (ROS) produced in excess by activated phagocyte NADPH oxidase (NOX2) can accentuate inflammatory responses. Thus, we investigated if Anv-water soluble polysaccharides could modulate primary human neutrophil oxidative burst in vitro.


METHODS
Human neutrophils were isolated from fresh whole blood and O2.-generation was measured by cytochrome c reduction assays. Western blots were used to analyse the translocation of PKC, p47phox(a key component of NOX2 activity) to neutrophil plasma membrane. Also, myeloperoxidase (MPO) release in the extracellular medium was studied by western blots. Flow cytometric analysis was used to detect CD11b membrane expression.


RESULTS
Water soluble polysaccharides from Anv dose-dependently inhibited N-formyl-methionyl-leucyl-phenylalanine (fMLF)- and phorbol myristate acetate (PMA)-induced O2.-generation by human neutrophils. Moreover, Anv-polysaccharides strongly inhibited PMA-induced PKCβ and p47phoxtranslocation to membranes and p47phoxphosphorylation on Ser328, a main PKC target. In contrast, polysaccharides extract from Zygophyllum gaetulum plant, which is also used as a traditional North African medicine against inflammatory diseases, was ineffective on this PKCβ-p47phox pathway. Further, Anv inhibited important neutrophil degranulation markers corresponding to myeloperoxidase (MPO) release and CD11b membrane expression.


CONCLUSIONS
The process of down-regulating NADPH oxidase by polysaccharides extracts from Anv provides new insights into the mechanism of Anv's anti-inflammatory actions.
INSERM, U1149, CNRS-ERL8252, Centre de Recherche sur l'Inflammation (CRI), 16 rue Henri Huchard, 75018, Paris, France.|Université Paris Diderot, Sorbonne, 75018, Paris, France.|Laboratoire d'Excellence Inflamex, Faculté de Médecine, Site Xavier Bichat, 75018, Paris, France.|Laboratoire de Biologie, eau et Environnement - Département de Biologie, Faculté SNV/ STU, Université 8 mai 1945 - Guelma, BP 401, Guelma, 24000, Algeria.
Hanane
H
Boukemara
INSERM, U1149, CNRS-ERL8252, Centre de Recherche sur l'Inflammation (CRI), 16 rue Henri Huchard, 75018, Paris, France.|Université Paris Diderot, Sorbonne, 75018, Paris, France.|Laboratoire d'Excellence Inflamex, Faculté de Médecine, Site Xavier Bichat, 75018, Paris, France.|AP-HP, HUPNVS Bichat-Claude Bernard, UF Dysfonctionnements Immunitaires, 75018, Paris, France.
Margarita
M
Hurtado-Nedelec
INSERM, U1149, CNRS-ERL8252, Centre de Recherche sur l'Inflammation (CRI), 16 rue Henri Huchard, 75018, Paris, France.|Université Paris Diderot, Sorbonne, 75018, Paris, France.|Laboratoire d'Excellence Inflamex, Faculté de Médecine, Site Xavier Bichat, 75018, Paris, France.
Viviana
V
Marzaioli
Laboratoire de Biologie, eau et Environnement - Département de Biologie, Faculté SNV/ STU, Université 8 mai 1945 - Guelma, BP 401, Guelma, 24000, Algeria.
Dalila
D
Bendjeddou
INSERM, U1149, CNRS-ERL8252, Centre de Recherche sur l'Inflammation (CRI), 16 rue Henri Huchard, 75018, Paris, France.|Université Paris Diderot, Sorbonne, 75018, Paris, France.|Laboratoire d'Excellence Inflamex, Faculté de Médecine, Site Xavier Bichat, 75018, Paris, France.
Jamel
J
El Benna
INSERM, U1149, CNRS-ERL8252, Centre de Recherche sur l'Inflammation (CRI), 16 rue Henri Huchard, 75018, Paris, France. jean-claude.marie@inserm.fr.|Université Paris Diderot, Sorbonne, 75018, Paris, France. jean-claude.marie@inserm.fr.|Laboratoire d'Excellence Inflamex, Faculté de Médecine, Site Xavier Bichat, 75018, Paris, France. jean-claude.marie@inserm.fr.
Jean-Claude
JC
Marie
D000352:Africa, Northern; D019659:Asteraceae / Q000737:chemistry*; D015550:Cell Degranulation / Q000187:drug effects; D002478:Cells, Cultured; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000187:drug effects; D009504:Neutrophils / Q000187:drug effects*; D018384:Oxidative Stress / Q000187:drug effects*; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D010946:Plants, Medicinal / Q000737:chemistry*
D010936:Plant Extracts
Anvillea garcinii; Inflammation; NADPH oxidase; North Africa; Zygophyllum gaetulum