Sudan Black B, The Specific Histochemical Stain for Lipofuscin: A Novel Method to Detect Senescent Cells.
The Sudan-Black-B (SBB) histochemical stain is well known to specifically react against lipofuscin, an aggregate of oxidized proteins, lipids, and metals. Lipofuscin is related to many ageing processes. It is also known to accumulate in senescent cells. We recently proved that lipofuscin detection, when applying the SBB staining, is highly specific for the visualization of senescent cells. Here, we present in detail this SBB method that can detect senescent cells in any material, irrespective of its preparation. This provides unique advantages not only in understanding physiological processes and the pathophysiology of various diseases but also in estimating the response to therapeutic interventions.
Molecular Carcinogenesis Group, Department of Histology and Embryology, Medical School, National and Kapodistrian University of Athens, Mikras Asias 75, Athens, 11527, Greece.
Konstantinos
K
Evangelou
Molecular Carcinogenesis Group, Department of Histology and Embryology, Medical School, National and Kapodistrian University of Athens, Mikras Asias 75, Athens, 11527, Greece. vgorg@med.uoa.gr.|Biomedical Research Foundation, Academy of Athens, Athens, Greece. vgorg@med.uoa.gr.|Faculty of Biology, Medicine and Health Manchester Cancer Research Centre, Manchester Academic Health Sciences Centre, University of Manchester, Manchester, UK. vgorg@med.uoa.gr.
Vassilis G
VG
Gorgoulis
D001391:Azo Compounds / Q000378:metabolism*; D002460:Cell Line; D016922:Cellular Senescence / Q000235:genetics; D004396:Coloring Agents / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D008062:Lipofuscin / Q000235:genetics / Q000378:metabolism*; D008297:Male; D009281:Naphthalenes / Q000378:metabolism*; D012669:Seminal Vesicles / Q000378:metabolism
D001391:Azo Compounds; D004396:Coloring Agents; D008062:Lipofuscin; D009281:Naphthalenes; C016118:Sudan Black B
Archival material; Fresh material; Histochemical stain; Lipofuscin; Paraffin; Senescence; Sudan-Black-B