Lipoprotein lipase gene-deficient mice with hypertriglyceridaemia associated with acute pancreatitis.
To investigate the severity of pancreatitis in lipoprotein lipase (LPL)-deficient hypertriglyceridaemic (HTG) heterozygous mice and to establish an experimental animal model for HTG pancreatitis study.
LPL-deficient HTG heterozygous mice were rescued by somatic gene transfer and mated with wild-type mice. The plasma amylase, triglyceride, and pathologic changes in the pancreas of the LPL-deficient HTG heterozygous mice were compared with those of wild-type mice to assess the severity of pancreatitis. In addition, acute pancreatitis (AP) was induced by caerulein (50 µg/kg) for further assessment.
The levels of plasma amylase and triglyceride were significantly higher in the LPL-deficient HTG heterozygous mice. According to the pancreatic histopathologic scores, the LPL-deficient HTG heterozygous mice showed more severe pathologic damage than the wild-type mice.
Lipoprotein lipase deficient heterozygous mice developed severe caerulein-induced pancreatitis. In addition, their high triglyceride levels were stable. Therefore, LPL-deficient HTG heterozygous mice are a useful experimental model for studying HTG pancreatitis.
MD, Department of Gastroenterology, Shanghai Tenth People's Hospital, Tongji University School of Medicine, Shanghai, China. Conception and design of the study, analysis of data, manuscript writing, final approved.
Maochun
M
Tang
MD, Department of Gastroenterology, Shanghai Tenth People's Hospital, Tongji University School of Medicine, Shanghai, China. Conception and design of the study, analysis of data, manuscript writing, final approved.
Pengfei
P
Zong
MD, Department of Gastroenterology, Shanghai Tenth People's Hospital, Tongji University School of Medicine, Shanghai, China. Conception and design of the study, analysis of data, manuscript writing, final approved.
Ting
T
Zhang
MD, Department of Gastroenterology, Shanghai Tenth People's Hospital, Tongji University School of Medicine, Shanghai, China. Conception and design of the study, analysis of data, manuscript writing, final approved.
Dongyan
D
Wang
MD, Institute of Cardiovascular Science, Peking University Healthy Science Center, Beijing, China. Analysis of data, final approved.
Yuhui
Y
Wang
MD, Department of Gastroenterology, Shanghai Tenth People's Hospital, Tongji University School of Medicine, Shanghai, China. Conception and design of the study, analysis of data, manuscript writing, final approved.
Yan
Y
Zhao
D000208:Acute Disease; D000681:Amylases / Q000097:blood; D000818:Animals; D004195:Disease Models, Animal; D005260:Female; D006579:Heterozygote; D008072:Hyperlipoproteinemia Type I / Q000150:complications* / Q000235:genetics; D015228:Hypertriglyceridemia / Q000150:complications*; D008810:Mice, Inbred C57BL; D010195:Pancreatitis / Q000209:etiology* / Q000473:pathology; D012720:Severity of Illness Index; D013997:Time Factors; D014280:Triglycerides / Q000097:blood
D014280:Triglycerides; D000681:Amylases