An overview on different strategies for the stemness maintenance of MSCs.
Recent evidence suggests that mesenchymal stem cells (MSCs) have promising therapeutic potential for a broad range of diseases. Because the percentage of MSCs obtained from tissues is very low for cell therapy applications, ex vivo expansion of MSCs is necessary, but aging, loss of stemness and undesired differentiation of them during in vitro cultivation reduces their effectiveness. For achieving ideal therapeutic potential of MSCs in tissue regenerative purposes, it is necessary to retain their stemness properties in vitro. This review emphasis on the last updates in preserving the self-renewal capability of stem cells through in vitro expansion with different parameters.
a Stem Cell Research Center, Tabriz University of Medical Sciences , Tabriz , Iran.|b Department of Medical Biotechnology, Faculty of Advanced Medical Sciences , Tabriz University of Medical Sciences , Tabriz , Iran.
Khatereh
K
Saei Arezoumand
a Stem Cell Research Center, Tabriz University of Medical Sciences , Tabriz , Iran.|b Department of Medical Biotechnology, Faculty of Advanced Medical Sciences , Tabriz University of Medical Sciences , Tabriz , Iran.
Effat
E
Alizadeh
a Stem Cell Research Center, Tabriz University of Medical Sciences , Tabriz , Iran.|b Department of Medical Biotechnology, Faculty of Advanced Medical Sciences , Tabriz University of Medical Sciences , Tabriz , Iran.
Younes
Y
Pilehvar-Soltanahmadi
c Department of Medical Biotechnologies , Universita degli Studi di siena , Siena , Italy.
Mohammad
M
Esmaeillou
a Stem Cell Research Center, Tabriz University of Medical Sciences , Tabriz , Iran.|b Department of Medical Biotechnology, Faculty of Advanced Medical Sciences , Tabriz University of Medical Sciences , Tabriz , Iran.
Nosratollah
N
Zarghami
D000818:Animals; D001672:Biocompatible Materials / Q000494:pharmacology; D018929:Cell Culture Techniques; D049109:Cell Proliferation; D064987:Cell- and Tissue-Based Therapy; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism
D001672:Biocompatible Materials
MSCs; Stemness; differentiation; regenerative medicine