[Novel miRNA genes hypermethylated in breast cancer].
MicroRNAs play an important role in the regulation of expression of many genes involved in cancer pathogenesis. One of the causes of miRNA level deregulation in tumors is the methylation of CpG islands in the promoter regions of the genes that encode them. Hypermethylation may lead to the suppression of miRNA gene expression and, as a consequence, to a decrease in their inhibitory effect on target gene mRNAs. A search for new miRNA genes hypermethylated in breast cancer has been carried out in the present study. The methylation of five miRNA genes associated with breast cancer (miR-132, miR-1258, miR-107, miR-130b, miR-137) has been as studied using a representative set of 41 breast cancer samples by methylation-specific PCR. Three new genes, MIR-132, MIR-137 and MIR-1258, with a high frequency of hypermethylation (41, 37 and 34%, respectively) have been identified in breast cancer. The methylation of these genes in the breast tissues of ten donors without cancer pathology in anamnesis was only found in single cases. These results enable the involvement of three miRNAs (miR-132, miR-137, miR-1258) and the methylation of the genes that encode them in the pathogenesis of breast cancer to be suggested.
Institute of General Pathology and Pathophysiology, Moscow, 125315 Russia.|loginov7w@gmail.com.
V I
VI
Loginov
Institute of General Pathology and Pathophysiology, Moscow, 125315 Russia.
A M
AM
Burdennyy
Institute of General Pathology and Pathophysiology, Moscow, 125315 Russia.
I V
IV
Pronina
Institute of General Pathology and Pathophysiology, Moscow, 125315 Russia.
V V
VV
Khokonova
Institute of General Pathology and Pathophysiology, Moscow, 125315 Russia.
S V
SV
Kurevljov
Blokhin Russian Cancer Research Center, Moscow, 115478 Russia.
T P
TP
Kazubskaya
Blokhin Russian Cancer Research Center, Moscow, 115478 Russia.
N E
NE
Kushlinskii
Institute of General Pathology and Pathophysiology, Moscow, 125315 Russia.|eleonora10_45@mail.ru.
E A
EA
Braga
D001943:Breast Neoplasms / Q000235:genetics / Q000378:metabolism*; D019175:DNA Methylation; D004273:DNA, Neoplasm / Q000235:genetics / Q000378:metabolism*; D005260:Female; D052138:Genes, Neoplasm; D006801:Humans; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D012334:RNA, Neoplasm / Q000235:genetics / Q000378:metabolism*
D004273:DNA, Neoplasm; D035683:MicroRNAs; D012334:RNA, Neoplasm
breast cancer; hypermethylation; miRNA genes