Bioactivity of an antihypertensive peptide expressed in Chlamydomonas reinhardtii.
In this study, we developed a transplastomic C. reinhardtii strain that accumulates anti-hypertensive peptides. Tandem repeats of VLPVP peptide were included. PCR analysis confirmed the presence of the transgene in the modified strains. After in vitro digestion of biomass of a recombinant C. reinhardtii strain the VLVPV peptide was identified and quantified by HPLC. The highest expression line produced 0.292mg of recombinant protein per mg of freeze-dried biomass. Intragastric administration of the genetically modified strain to spontaneous hypertensive rats at a dose of 30mg/kg of body weight of recombinant protein significantly reduced systolic blood pressure. At the same dose, the recombinant protein exerts an ACE-inhibitory effect. This is the first study that indicates the potential of this microalga producing an antihypertensive peptide as a dietary supplement for hypertension patients.
Facultad de Ciencias Químicas, Universidad Autónoma de San Luis Potosí, Av. Dr. Manuel Nava 6, SLP 78210, Mexico.
Celma Estefanía
CE
Ochoa-Méndez
Facultad de Ciencias Químicas, Universidad Autónoma de San Luis Potosí, Av. Dr. Manuel Nava 6, SLP 78210, Mexico.
Ignacio
I
Lara-Hernández
Facultad de Ciencias Químicas, Universidad Autónoma de San Luis Potosí, Av. Dr. Manuel Nava 6, SLP 78210, Mexico.
Luzmila Martínez
LM
González
Facultad de Ciencias Químicas, Universidad Autónoma de San Luis Potosí, Av. Dr. Manuel Nava 6, SLP 78210, Mexico.
Patricia
P
Aguirre-Bañuelos
UBIMED, FES-Iztacala, Universidad Nacional Autónoma de México, Av. de los Barrios 1, Tlalnepantla 54090, Mexico.
Maximiliano
M
Ibarra-Barajas
UBIMED, FES-Iztacala, Universidad Nacional Autónoma de México, Av. de los Barrios 1, Tlalnepantla 54090, Mexico.
Patricia
P
Castro-Moreno
Facultad de Ciencias Químicas, Universidad Autónoma de San Luis Potosí, Av. Dr. Manuel Nava 6, SLP 78210, Mexico.
Omar
O
González-Ortega
Facultad de Ciencias Químicas, Universidad Autónoma de San Luis Potosí, Av. Dr. Manuel Nava 6, SLP 78210, Mexico. Electronic address: ruth_soria2@hotmail.com.
Ruth Elena
RE
Soria-Guerra
D000806:Angiotensin-Converting Enzyme Inhibitors; D000818:Animals; D000959:Antihypertensive Agents / Q000494:pharmacology*; D001794:Blood Pressure / Q000187:drug effects; D016825:Chlamydomonas reinhardtii; D006973:Hypertension / Q000188:drug therapy* / Q000503:physiopathology; D008297:Male; D009842:Oligopeptides / Q000096:biosynthesis / Q000235:genetics / Q000302:isolation & purification / Q000494:pharmacology*; D051381:Rats; D011918:Rats, Inbred SHR; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics / Q000302:isolation & purification / Q000494:pharmacology*
D000806:Angiotensin-Converting Enzyme Inhibitors; D000959:Antihypertensive Agents; D009842:Oligopeptides; D011994:Recombinant Proteins; C530287:valyl-leucyl-prolyl-valyl-proline
Bioactive peptides; Hypertension; Microalgae; Spontaneous hypertensive rats