DNA Nanostructure as Smart Carriers for Drug Delivery.
Self-assembled DNA nanostructures have recently emerged as a type of drug delivery carriers due to their suitable sizes, well-defined nanoscale shapes, precise spatial addressability, and excellent biocompatibility. Here, we describe practical procedures in detail for the design and construction of DNA nanostructures with different width and patterns by long rolling circle amplification (RCA) strands and a few short staples, and provide practical guidance and troubleshooting advice for delivering CpG immunostimulatory drugs with these RCA based DNA nanostructures.
Laboratory of Physical Biology, Chinese Academy of Sciences, Shanghai Institute of Applied Physics, Shanghai, P.O. Box 800-204, 201800, China.|Key Laboratory of Synthetic and Natural Function Molecule Chemistry of Ministry of Education, College of Chemistry and Material Science, Northwest University, Xi'an, 710127, People's Republic of China.
Xiangyuan
X
Ouyang
Laboratory of Physical Biology, Chinese Academy of Sciences, Shanghai Institute of Applied Physics, Shanghai, P.O. Box 800-204, 201800, China. iamjchao@njupt.edu.cn.|Key Laboratory for Organic Electronics and Information Displays & Institute of Advanced Materials (IAM), Jiangsu National Synergetic Innovation Center for Advanced Materials (SICAM), Nanjing University of Posts and Telecommunications, Nanjing, Jiangsu, 210023, China. iamjchao@njupt.edu.cn.
Jie
J
Chao
Key Laboratory for Organic Electronics and Information Displays & Institute of Advanced Materials (IAM), Jiangsu National Synergetic Innovation Center for Advanced Materials (SICAM), Nanjing University of Posts and Telecommunications, Nanjing, Jiangsu, 210023, China.
Shao
S
Su
Laboratory of Physical Biology, Chinese Academy of Sciences, Shanghai Institute of Applied Physics, Shanghai, P.O. Box 800-204, 201800, China. fchh@sinap.ac.cn.
Chunhai
C
Fan
D004247:DNA / Q000737:chemistry*; D004337:Drug Carriers / Q000737:chemistry*; D016503:Drug Delivery Systems / Q000379:methods; D049329:Nanostructures / Q000737:chemistry*; D036103:Nanotechnology / Q000379:methods; D021141:Nucleic Acid Amplification Techniques / Q000379:methods
D004337:Drug Carriers; D004247:DNA
CpG immunostimulatory drugs; DNA nanostructures; Drug delivery; Rolling circle amplification