Improvement of Electroacupuncture on APP/PS1 Transgenic Mice in Spatial Learning and Memory Probably due to Expression of Aβand LRP1 in Hippocampus.
Objectives. To explore the alterations ofβ-amyloid (Aβ) and low density lipoprotein receptor-related protein-1 (LRP1) in APP/PS1 mice after electroacupuncture (EA) treatment and further to explore the mechanism.Methods. Forty 6-month-old APP/PS1 mice were randomly divided into a model group and an EA group, with twenty wild-type mice used as a normal control group. Mice in the EA group were treated with EA at GV 20 (băi huì) and bilateral KI 1 (yŏng quán) acupoints for 6 weeks. The Morris water maze was applied to assess the spatial memory in behavior. Immunohistochemistry (IHC), ELISA, Western blotting, and so forth were used to observe the expression of LRP1 and Aβ.Results. The Morris water maze test showed that, compared with the normal control group, the model group's learning and memory capabilities were significantly decreased (P< 0.05;P< 0.01). The EA group was reversed (P< 0.05;P< 0.01). The hippocampal expression of Aβin the EA group was significantly decreased compared to the model group (P< 0.01). The expression of LRP1 in the model group was significantly lower than that in the normal control group (P< 0.01); the expression in the EA group was significantly higher than that in the model group (P< 0.01).Conclusions. EA therapy can improve the learning and memory capabilities of APP/PS1 mice. The underlying mechanism may lie in the upregulation of an Aβtransport receptor and LRP1.
School of Acupuncture, Moxibustion and Tuina, Beijing University of Chinese Medicine, No. 11 Bei San Huan Dong Lu, Chaoyang District, Beijing 100029, China.
Xin
0000-0002-7759-9117
X
Wang
Community Health Service Administration Center of Dongcheng District, No. 192-1 Chaoyangmen Internal Street, Dongcheng District, Beijing 100053, China.
Yanhuan
0000-0001-8507-7784
Y
Miao
School of Acupuncture, Moxibustion and Tuina, Beijing University of Chinese Medicine, No. 11 Bei San Huan Dong Lu, Chaoyang District, Beijing 100029, China.
Jiawula
J
Abulizi
School of Acupuncture, Moxibustion and Tuina, Beijing University of Chinese Medicine, No. 11 Bei San Huan Dong Lu, Chaoyang District, Beijing 100029, China.
Fu
F
Li
School of Acupuncture, Moxibustion and Tuina, Beijing University of Chinese Medicine, No. 11 Bei San Huan Dong Lu, Chaoyang District, Beijing 100029, China.
Yuping
0000-0002-0917-0773
Y
Mo
School of Acupuncture, Moxibustion and Tuina, Beijing University of Chinese Medicine, No. 11 Bei San Huan Dong Lu, Chaoyang District, Beijing 100029, China.
Weiguo
0000-0002-1327-0582
W
Xue
School of Acupuncture, Moxibustion and Tuina, Beijing University of Chinese Medicine, No. 11 Bei San Huan Dong Lu, Chaoyang District, Beijing 100029, China.
Zhigang
0000-0003-0378-3496
Z
Li