Investigator®HDplex (Qiagen) reference population database for forensic use in Argentina.
Currently, autosomal Short Tandem Repeat (STR) markers represent the method of election in forensic human identification. Commercial kits of most common use nowadays -e.g. PowerPlex®Fusion, Promega Corp.; AmpFlSTR GlobalFiler, Thermofisher scientific; Investigator 24Plex QS,Qiagen-, allow the co-amplification of 23 highly polymorphic STR loci providing a high discrimination power in human identity testing. However, in complex kinship analysis and familial database searches involving distant relationships, additional DNA typing is often required in order to achieve well-founded conclusions. The recently developed kit Investigator®HDplex (Qiagen) co-amplify twelve autosomal STRs markers (D7S1517, D3S1744, D12S391, D2S1360, D6S474, D4S2366, D8S1132, D5S2500, D18S51, D21S2055, D10S2325, SE33), nine of which are not present in the above mentioned kits, providing a set of efficient supplementary markers for human identification purposes. In this study we genotyped a sample of 980 individuals from urban areas of ten Argentinean provinces using the Investigator®HDplex kit, aiming to provide forensic estimates for use in forensic casework and parentage testing in Argentina. We report reference allelic frequency databases for each of the provinces studied as well as for the combined samples. No deviation of Hardy-Weinberg equilibrium was observed. A reasonable discrimination capacity and power of exclusion was estimated which allowed predicting an acceptable forensic behavior of this kit, either to be used as the main STR panel for simple cases or as an auxiliary tool in complex cases. Additionally, population comparison tests showed that the studied samples are relatively homogeneous across the country for these STR set.
Servicio de Genética Forense, Poder Judicial de la Provincia de Entre Ríos, Entre Ríos, Argentina. Electronic address: gustavo_g_martinez@outlook.com.
Gustavo
G
Martínez
LIDMO, Córdoba, Argentina.
Alicia
A
Borosky
Servicio de Huellas Digitales Genéticas, Facultad de Farmacia y Bioquímica, U.B.A., Buenos Aires, Argentina.
Daniel
D
Corach
PRICAI-Fundación Favaloro, Buenos Aires, Argentina.
Cintia
C
Llull
Laboratorio de Genética Forense, Cuerpo Médico Forense y Criminalístico, Ministerio Público de la Provincia de Mendoza, Mendoza, Argentina.
Laura
L
Locarno
Asesoría Pericial, La Plata, Poder Judicial de la Provincia de Buenos Aires, Buenos Aires, Argentina.
Mercedes
M
Lojo
Laboratorio de Genética Forense, Cuerpo Médico Forense y Criminalístico, Ministerio Público de la Provincia de Mendoza, Mendoza, Argentina.
Miguel
M
Marino
Laboratorio Regional de Genética Forense del NOA,Departamento Médico-Poder Judicial de Jujuy, Jujuy, Argentina.
María Cecilia
MC
Miozzo
Instituto de Genética Forense, Poder Judicial de Córdoba, Córdoba, Argentina.
Nidia
N
Modesti
Instituto de Genética Forense, Poder Judicial de Córdoba, Córdoba, Argentina.
Carla
C
Pacharoni
Asesoría Pericial, La Plata, Poder Judicial de la Provincia de Buenos Aires, Buenos Aires, Argentina.
Juan Pablo
JP
Pilili
Laboratorio Regional de Genética Forense del NOA,Departamento Médico-Poder Judicial de Jujuy, Jujuy, Argentina.
María Isabel
MI
Ramella
Servicio de Huellas Digitales Genéticas, Facultad de Farmacia y Bioquímica, U.B.A., Buenos Aires, Argentina.
Andrea
A
Sala
Servicio de Genética Forense, Poder Judicial de la Provincia de Entre Ríos, Entre Ríos, Argentina.
Cecilia
C
Schaller
LIDMO, Córdoba, Argentina; EAAF Forensic Genetic Laboratory, Córdoba, Argentina.
Carlos
C
Vullo
PRICAI-Fundación Favaloro, Buenos Aires, Argentina. Electronic address: utoscanini@pricai.com.ar.
Ulises
U
Toscanini
D001118:Argentina; D016172:DNA Fingerprinting; D030561:Databases, Nucleic Acid; D005787:Gene Frequency; D005828:Genetics, Population; D005838:Genotype; D006801:Humans; D018895:Microsatellite Repeats
Allele frequencies; Argentina; Forensic genetics; Investigator(®) HDplex; Population genetics; STR