Adeno-Associated Virus 5 Transduces Adipose-Derived Stem Cells with Greater Efficacy Than Other Adeno-Associated Viral Serotypes.
Adipose-derived stem cells (ASCs) have shown potential in the treatment of a myriad of diseases; however, infusion of cells alone is unlikely to provide the full range of potential therapeutic applications. Transient genetic manipulation of ASCs could increase their repair and regeneration characteristics in a disease-specific context, essentially transforming them into drug-eluting depots. The goal of this study was to determine the optimal parameters necessary to transduce ASCs with recombinant adeno-associated virus (rAAV), an approved gene therapy vector that has never been associated with disease. Transduction and duration of gene expression of the most common recombinant AAV vectors were tested in this study. Among all tested serotypes, rAAV5 resulted in both the highest and longest term expression. Furthermore, we determined the glycosylation profile of ASCs before and after neuraminidase treatment and demonstrate that rAAV5 transduction requires plasma membrane-associated sialic acid. Future studies will focus on the optimization of gene delivery to ASCs, using rAAV5 as the vector of choice, to drive biological drug delivery, engraftment, and disease correction.
1 Department of Biological Sciences, Wright State University.
Priyanka
P
Sharma
2 Department of Orthopedic Surgery, Sports Medicine and Rehabilitation, Boonshoft School of Medicine, Wright State University.|3 Wright State Physicians Plastic Surgery, Miami Valley Hospital, Dayton, Ohio.
Sunishka M
SM
Wimalawansa
2 Department of Orthopedic Surgery, Sports Medicine and Rehabilitation, Boonshoft School of Medicine, Wright State University.
Gregory C
GC
Gould
2 Department of Orthopedic Surgery, Sports Medicine and Rehabilitation, Boonshoft School of Medicine, Wright State University.|3 Wright State Physicians Plastic Surgery, Miami Valley Hospital, Dayton, Ohio.
R Michael
RM
Johnson
1 Department of Biological Sciences, Wright State University.|2 Department of Orthopedic Surgery, Sports Medicine and Rehabilitation, Boonshoft School of Medicine, Wright State University.
Katherine J D A
KJ
Excoffon
D000273:Adipose Tissue / Q000166:cytology; D000818:Animals; D000229:Dependovirus / Q000235:genetics*; D018014:Gene Transfer Techniques; D015316:Genetic Therapy; D005822:Genetic Vectors / Q000235:genetics; D006801:Humans; D065288:Serogroup; D013234:Stem Cells; D014161:Transduction, Genetic
adeno-associated virus; adipose-derived stem cells; gene therapy; stem cell therapy