NanoLC-MS coupling of liquid microjunction microextraction for on-tissue proteomic analysis.
Mass spectrometry (MS)-based microproteomics on localized regions of tissue sections was achieved by direct coupling of liquid microjunction microextraction with a nanoscale liquid chromatography-tandem MS, resulting in the identification of >500 protein groups from a region as small as 250μm in diameter representing only a few hundred of cells. The method was applied on the examination of benign and tumor regions initially defined by imaging mass spectrometry (IMS) analysis of a consecutive high grade serous ovarian tumor tissue section. Results identified the higher abundance of eukaryotic translation initiation factors eIF4A, its isoform eIF4A2, and eIF5A and its isoform eIF5A2, and lower abundance of actin-binding proteins OBSCN, TAGLN and CNN3 on tumor regions, concomitant with previous findings. This demonstrates the use of the method for downstream characterization of distinct regions identified by IMS. This article is part of a Special Issue entitled: MALDI Imaging, edited by Dr. Corinna Henkel and Prof. Peter Hoffmann.
Université de Lille 1, Inserm U-1192, Protéomique, Réponse Inflammatoire et Spectrométrie de Masse (PRISM), F-59000 Lille, France. Electronic address: jusal.quanico@univ-lille1.fr.
Jusal
J
Quanico
Université de Lille 1, Inserm U-1192, Protéomique, Réponse Inflammatoire et Spectrométrie de Masse (PRISM), F-59000 Lille, France. Electronic address: julien.franck@univ-lille1.fr.
Julien
J
Franck
Université de Lille 1, Inserm U-1192, Protéomique, Réponse Inflammatoire et Spectrométrie de Masse (PRISM), F-59000 Lille, France. Electronic address: tristan.cardon@ed.univ-lille1.fr.
Tristan
T
Cardon
Université de Lille 1, Inserm U-1192, Laboratoire de Protéomique, Réponse Inflammatoire, Spectrométrie de Masse (PRISM), Department of Gynecology Oncology, Oscar Lambret Center, Lille, France. Electronic address: e-leblanc@o-lambret.fr.
Eric
E
Leblanc
Université de Lille 1, Inserm U-1192, Protéomique, Réponse Inflammatoire et Spectrométrie de Masse (PRISM), F-59000 Lille, France. Electronic address: maxence.wisztorski@univ-lille1.fr.
Maxence
M
Wisztorski
Université de Lille 1, Inserm U-1192, Protéomique, Réponse Inflammatoire et Spectrométrie de Masse (PRISM), F-59000 Lille, France. Electronic address: michel.salzet@univ-lille1.fr.
Michel
M
Salzet
Université de Lille 1, Inserm U-1192, Protéomique, Réponse Inflammatoire et Spectrométrie de Masse (PRISM), F-59000 Lille, France. Electronic address: isabelle.fournier@univ-lille1.fr.
Isabelle
I
Fournier
D005591:Chemical Fractionation / Q000379:methods; D002853:Chromatography, Liquid / Q000379:methods; D039601:Eukaryotic Initiation Factor-4A / Q000378:metabolism; D006801:Humans; D008840:Microfilament Proteins / Q000378:metabolism; D009369:Neoplasms / Q000175:diagnosis / Q000378:metabolism / Q000473:pathology; D010448:Peptide Initiation Factors / Q000378:metabolism; D011506:Proteins / Q000378:metabolism*; D020543:Proteome / Q000378:metabolism*; D040901:Proteomics / Q000379:methods; D016601:RNA-Binding Proteins / Q000378:metabolism; D053719:Tandem Mass Spectrometry / Q000379:methods; D000097574:Eukaryotic Translation Initiation Factor 5A
D039601:Eukaryotic Initiation Factor-4A; D008840:Microfilament Proteins; D010448:Peptide Initiation Factors; D011506:Proteins; D020543:Proteome; D016601:RNA-Binding Proteins; D000097574:Eukaryotic Translation Initiation Factor 5A
Imaging mass spectrometry; Liquid microjunction; Microextraction; Microproteomics; Ovarian cancer