High-density natural luffa sponge as anaerobic microorganisms carrier for degrading 1,1,1-TCA in groundwater.
Anaerobic microorganisms were applied to degrade organic contaminants in groundwater with permeable reactive barriers (PRBs). However, anaerobic microorganisms need to select optimal immobilizing material as carrier. The potential of high-density natural luffa sponge (HDLS) (a new variety of luffa) for the immobilization and protection of anaerobic microorganisms was investigated. The HDLS has a dense structure composed of a complicated interwoven fibrous network. Therefore, the abrasion rate of HDLS (0.0068 g s-1) was the smallest among the four carriers [HDLS, ordinary natural luffa sponge (OLS), polyurethane sponge (PS), and gel carrier AQUAPOROUSGEL (APG)]. The results suggest that it also had the greatest water retention (10.26 H2O-g dry carrier-g-1) and SS retention (0.21 g dry carrier-g-1). In comparison to well-established commercialized gel carrier APG, HDLS was of much better mechanical strength, hydrophilicity and stability. Microbial-immobilized HDLS also had the best performance for the remediation of 1,1,1-TCA simulated groundwater. Analysis of the clone libraries from microorganism-immobilized HDLS showed the HDLS could protect microorganisms from the toxicity of 1,1,1-TCA and maintain the stability of microbial community diversity. The mechanism of HDLS immobilizing and protecting microorganisms was proposed as follows. The HDLS had a micron-scale honeycomb structure (30-40 μm) and an irregular ravine structure (4-20 μm), which facilitate the immobilization of anaerobic microorganisms and protect the anaerobic microorganisms.
School of Environmental Science and Engineering, Shanghai Jiao Tong University, Shanghai, 200240, People's Republic of China.
Wenbing
W
Wang
School of Environmental Science and Engineering, Shanghai Jiao Tong University, Shanghai, 200240, People's Republic of China. wuyanqing@sjtu.edu.cn.
Yanqing
Y
Wu
School of Environmental Science and Engineering, Shanghai Jiao Tong University, Shanghai, 200240, People's Republic of China.
Chi
C
Zhang
D000693:Anaerobiosis; D001673:Biodegradation, Environmental; D004800:Enzymes, Immobilized / Q000737:chemistry*; D060587:Groundwater; D057927:Hydrophobic and Hydrophilic Interactions; D029776:Luffa; D064307:Microbiota; D008855:Microscopy, Electron, Scanning; D011140:Polyurethanes / Q000737:chemistry; D014240:Trichloroethanes / Q000737:chemistry*; D014867:Water / Q000737:chemistry; D014871:Water Microbiology; D014874:Water Pollutants, Chemical; D018508:Water Purification / Q000379:methods*
D004800:Enzymes, Immobilized; D011140:Polyurethanes; D014240:Trichloroethanes; D014874:Water Pollutants, Chemical; D014867:Water; C024566:1,1,1-trichloroethane
Anaerobic microorganism; Biodegradation; Groundwater contamination; Immobilization medium; Natural luffa sponge