Characterization of the Expression of Basigin Gene Products Within the Pineal Gland of Mice.
The expression of Basigin gene products and monocarboxylate transporter-1 (MCT1) has been investigated within the mammalian neural retina and suggests a role for these proteins in cellular metabolism within that tissue. The purpose of the present study was to investigate the expression of these same proteins in the pineal gland of the mouse brain. Mouse pineal gland and neural retina RNA and protein were subjected to quantitative reverse transcription-polymerase chain reaction and immunoblotting analyses. In addition, paraffin-embedded sections of each tissue were analyzed for expression of Basigin gene products and MCT1 via immunohistochemistry. The results indicate that MCT1 and Basigin variant-2, but not Basigin variant-1, are expressed within the mouse pineal gland. The expression of Basigin variant-2 and MCT1 was localized to the capsule surrounding the gland. The position and relative amounts of the gene products suggest that they play a much less prominent role within the pineal gland than in the neural retina.
Department of Biology, University of North Florida, 1 UNF Drive, Jacksonville, FL, 32224, USA.
Derek
D
Tokar
Whitney Laboratory for Marine Biosciences and the Department of Anatomy and Cell Biology, University of Florida, St. Augustine, FL, USA.
Leslie
L
van Ekeris
Whitney Laboratory for Marine Biosciences and the Department of Anatomy and Cell Biology, University of Florida, St. Augustine, FL, USA.
Paul J
PJ
Linser
Department of Biology, University of North Florida, 1 UNF Drive, Jacksonville, FL, 32224, USA. j.ochrietor@unf.edu.
Judith D
JD
Ochrietor
D000818:Animals; D051926:Basigin / Q000235:genetics* / Q000378:metabolism; D005786:Gene Expression Regulation; D008810:Mice, Inbred C57BL; D027501:Monocarboxylic Acid Transporters / Q000378:metabolism; D010870:Pineal Gland / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D027981:Symporters / Q000378:metabolism; D000099233:Monocarboxylate Transport Protein 1
D051926:Basigin; D027501:Monocarboxylic Acid Transporters; D012333:RNA, Messenger; D027981:Symporters; D000099233:Monocarboxylate Transport Protein 1
Basigin gene products; Monocarboxylate transporter-1; Neural retina; Pineal gland