Deep Sequencing Analysis Reveals the Mycoviral Diversity of the Virome of an Avirulent Isolate of Rhizoctonia solani AG-2-2 IV.
Rhizoctonia solani represents an important plant pathogenic Basidiomycota species complex and the host of many different mycoviruses, as indicated by frequent detection of dsRNA elements in natural populations of the fungus. To date, eight different mycoviruses have been characterized in Rhizoctonia and some of them have been reported to modulate its virulence. DsRNA extracts of the avirulent R. solani isolate DC17 (AG2-2-IV) displayed a diverse pattern, indicating multiple infections with mycoviruses. Deep sequencing analysis of the dsRNA extract, converted to cDNA, revealed that this isolate harbors at least 17 different mycovirus species. Based on the alignment of the conserved RNA-dependent RNA-polymerase (RdRp) domain, this viral community included putative members of the families Narnaviridae, Endornaviridae, Partitiviridae and Megabirnaviridae as well as of the order Tymovirales. Furthermore, viruses, which could not be assigned to any existing family or order, but showed similarities to so far unassigned species like Sclerotinia sclerotiorum RNA virus L, Rhizoctonia solani dsRNA virus 1, Aspergillus foetidus slow virus 2 or Rhizoctonia fumigata virus 1, were identified. This is the first report of a fungal isolate infected by 17 different viral species and a valuable study case to explore the diversity of mycoviruses infecting R. solani.
Institute of Sugar Beet Research, D-37077, Göttingen, Germany.
Anika
A
Bartholomäus
Institute for Genome Research and Systems Biology, CeBiTec, Bielefeld University, D-33501, Bielefeld, Germany.
Daniel
D
Wibberg
Institute for Genome Research and Systems Biology, CeBiTec, Bielefeld University, D-33501, Bielefeld, Germany.
Anika
A
Winkler
Institute for Genome Research and Systems Biology, CeBiTec, Bielefeld University, D-33501, Bielefeld, Germany.
Alfred
A
Pühler
Institute for Genome Research and Systems Biology, CeBiTec, Bielefeld University, D-33501, Bielefeld, Germany.
Andreas
A
Schlüter
Institute of Sugar Beet Research, D-37077, Göttingen, Germany.
Mark
M
Varrelmann
D000595:Amino Acid Sequence; D018076:DNA, Complementary / Q000235:genetics; D000067148:Fungal Viruses / Q000235:genetics*; D016679:Genome, Viral / Q000235:genetics*; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods; D010802:Phylogeny; D012328:RNA Viruses / Q000235:genetics; D012330:RNA, Double-Stranded / Q000235:genetics; D012367:RNA, Viral / Q000235:genetics; D012324:RNA-Dependent RNA Polymerase / Q000378:metabolism; D012232:Rhizoctonia / Q000821:virology*; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA
D018076:DNA, Complementary; D012330:RNA, Double-Stranded; D012367:RNA, Viral; D012324:RNA-Dependent RNA Polymerase