Expression of interferon-stimulated gene ISG15 and ubiquitination enzymes is upregulated in peripheral blood monocyte during early pregnancy in dairy cattle.
Pregnancy recognition is associated with the upregulation of the great number of genes in ruminants. One of these genes is interferon-stimulatory gene (ISG15), an ubiquitin-like protein that mediates the conjugation of different proteins through its ISGylation enzymes UBE1L and UBCH8. We demonstrate that ISG15 and its ISGlyation enzymes UBE1L and UBCH8 are differentially regulated in pregnant and non-pregnant cows. The mRNA and/or protein levels of ISG15, UBE1L and UBCH8 increased significantly (p<0.05) after treatment of PBMCs with different concentrations of interferon-tau (IFN-τ). To correlate these changes with pregnancy, cows were synchronized and artificially inseminated on day 0 (AI group). Cows that were not artificially inseminated served at the control (NON-AI group). Blood was collected from cows of both groups on day 18, and the mRNA levels of ISG15, UBE1L and UBCH8 in PBMCs were analyzed and compared between groups. Pregnancy was diagnosed on day 45 by rectal palpation. Compared with non-pregnant cows and the NON-AI group, the mRNA levels of ISG15, UBE1L and UBCH8 increased significantly (p<0.05) in pregnant cows. In conclusion, the secretion of IFN-τ by the conceptus acts on PBMCs to upregulate the interferon-stimulated gene ISG15, thereby activating UBE1L and UBCH8. Thus ISG15, UBE1l and UBCH8 genes are significantly upregulated in the AI- pregnant cows.
Laboratory of Animal Embryonic Biotechnology, National Engineering Laboratory for Animal Breeding, Key Laboratory of Animal Genetics, Breeding, and Reproduction of the Ministry of Agriculture, College of Animal Science and Technology, China Agricultural University, Beijing, China.
Ihtesham Ul
IU
Haq
Laboratory of Animal Embryonic Biotechnology, National Engineering Laboratory for Animal Breeding, Key Laboratory of Animal Genetics, Breeding, and Reproduction of the Ministry of Agriculture, College of Animal Science and Technology, China Agricultural University, Beijing, China.
Ying
Y
Han
College of Veterinary Medicine, China Agricultural University, Beijing, China.
Tariq
T
Ali
Laboratory of Animal Embryonic Biotechnology, National Engineering Laboratory for Animal Breeding, Key Laboratory of Animal Genetics, Breeding, and Reproduction of the Ministry of Agriculture, College of Animal Science and Technology, China Agricultural University, Beijing, China.
Yingzheng
Y
Wang
Laboratory of Animal Embryonic Biotechnology, National Engineering Laboratory for Animal Breeding, Key Laboratory of Animal Genetics, Breeding, and Reproduction of the Ministry of Agriculture, College of Animal Science and Technology, China Agricultural University, Beijing, China.
Hui
H
Gao
Laboratory of Animal Embryonic Biotechnology, National Engineering Laboratory for Animal Breeding, Key Laboratory of Animal Genetics, Breeding, and Reproduction of the Ministry of Agriculture, College of Animal Science and Technology, China Agricultural University, Beijing, China.
Lu
L
Lin
Laboratory of Animal Embryonic Biotechnology, National Engineering Laboratory for Animal Breeding, Key Laboratory of Animal Genetics, Breeding, and Reproduction of the Ministry of Agriculture, College of Animal Science and Technology, China Agricultural University, Beijing, China.
Yi
Y
Wu
Laboratory of Animal Embryonic Biotechnology, National Engineering Laboratory for Animal Breeding, Key Laboratory of Animal Genetics, Breeding, and Reproduction of the Ministry of Agriculture, College of Animal Science and Technology, China Agricultural University, Beijing, China.
Shuaishuai
S
Wu
Laboratory of Animal Embryonic Biotechnology, National Engineering Laboratory for Animal Breeding, Key Laboratory of Animal Genetics, Breeding, and Reproduction of the Ministry of Agriculture, College of Animal Science and Technology, China Agricultural University, Beijing, China. Electronic address: zengsm@cau.edu.cn.
Shenming
S
Zeng
D000818:Animals; D002417:Cattle; D016207:Cytokines / Q000235:genetics* / Q000378:metabolism; D005260:Female; D007370:Interferon Type I / Q000494:pharmacology; D009000:Monocytes / Q000187:drug effects / Q000378:metabolism*; D011247:Pregnancy; D011257:Pregnancy Proteins / Q000494:pharmacology; D044764:Ubiquitin-Activating Enzymes / Q000235:genetics* / Q000378:metabolism; D054875:Ubiquitination / Q000187:drug effects; D014452:Ubiquitins / Q000235:genetics* / Q000378:metabolism; D015854:Up-Regulation / Q000187:drug effects
D016207:Cytokines; D007370:Interferon Type I; D011257:Pregnancy Proteins; D014452:Ubiquitins; C020947:interferon tau; D044764:Ubiquitin-Activating Enzymes
ISGylation cattle; Interferon-tau; Pregnancy; Ubiquitin