Characterization of glutathione peroxidase diversity in the symbiotic sea anemone Anemonia viridis.
Cnidarians living in symbiosis with photosynthetic dinoflagellates (commonly named zooxanthellae) are exposed to high concentrations of reactive oxygen species (ROS) upon illumination. To quench ROS production, both the cnidarian host and zooxanthellae express a full suite of antioxidant enzymes. Studying antioxidative balance is therefore crucial to understanding how symbiotic cnidarians cope with ROS production. We characterized glutathione peroxidases (GPx) in the symbiotic cnidarian Anemonia viridis by analysis of their isoform diversity, their activity distribution in the three cellular compartments (ectoderm, endoderm and zooxanthellae) and their involvement in the response to thermal stress. We identified a GPx repertoire through a phylogenetic analysis showing 7 GPx transcripts belonging to the A. viridis host and 4 GPx transcripts strongly related to Symbiodinium sp. The biochemical approach, used for the first time with a cnidarian species, allowed the identification of GPx activity in the three cellular compartments and in the animal mitochondrial fraction, and revealed a high GPx electrophoretic diversity. The symbiotic lifestyle of zooxanthellae requires more GPx activity and diversity than that of free-living species. Heat stress induced no modification of GPx activities. We highlight a high GPx diversity in A. viridis tissues by genomic and biochemical approaches. GPx activities represent an overall constitutive enzymatic pattern inherent to symbiotic lifestyle adaptation. This work allows the characterization of the GPx family in a symbiotic cnidarian and establishes a foundation for future studies of GPx in symbiotic cnidarians.
Sorbonne Universités, UPMC Univ Paris 06, Univ Antilles, Univ Nice Sophia Antipolis, CNRS, Evolution Paris Seine - Institut de Biologie Paris Seine (EPS - IBPS), 75005, Paris, France. Electronic address: pey@unice.fr.
Alexis
A
Pey
Sorbonne Universités, UPMC Univ Paris 06, Univ Antilles, Univ Nice Sophia Antipolis, CNRS, Evolution Paris Seine - Institut de Biologie Paris Seine (EPS - IBPS), 75005, Paris, France. Electronic address: thamilla.zamoum@unice.fr.
Thamilla
T
Zamoum
Sorbonne Universités, UPMC Univ Paris 06, Univ Antilles, Univ Nice Sophia Antipolis, CNRS, Evolution Paris Seine - Institut de Biologie Paris Seine (EPS - IBPS), 75005, Paris, France. Electronic address: richard.christen@unice.fr.
Richard
R
Christen
Sorbonne Universités, UPMC Univ Paris 06, Univ Antilles, Univ Nice Sophia Antipolis, CNRS, Evolution Paris Seine - Institut de Biologie Paris Seine (EPS - IBPS), 75005, Paris, France.
Pierre-Laurent
PL
Merle
Sorbonne Universités, UPMC Univ Paris 06, Univ Antilles, Univ Nice Sophia Antipolis, CNRS, Evolution Paris Seine - Institut de Biologie Paris Seine (EPS - IBPS), 75005, Paris, France. Electronic address: furla@unice.fr.
Paola
P
Furla
D000222:Adaptation, Physiological / Q000235:genetics; D000818:Animals; D004141:Dinoflagellida / Q000201:enzymology / Q000235:genetics / Q000254:growth & development; D020869:Gene Expression Profiling / Q000379:methods; D018507:Gene Expression Regulation, Developmental; D015971:Gene Expression Regulation, Enzymologic; D014644:Genetic Variation; D005979:Glutathione Peroxidase / Q000145:classification / Q000235:genetics* / Q000378:metabolism; D006358:Hot Temperature; D007527:Isoenzymes / Q000145:classification / Q000235:genetics / Q000378:metabolism; D010084:Oxidation-Reduction; D010802:Phylogeny; D017382:Reactive Oxygen Species / Q000378:metabolism; D012615:Sea Anemones / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development; D013053:Spectrophotometry; D013559:Symbiosis
D007527:Isoenzymes; D017382:Reactive Oxygen Species; D005979:Glutathione Peroxidase
Cnidaria; Glutathione peroxidase; Oxidative stress; Symbiosis; Zooxanthellae