A simple tool for tubing modification to improve spiral high-speed counter-current chromatography for protein purification.
A simple tool is introduced which can modify the shape of tubing to enhance the partition efficiency in high-speed countercurrent chromatography. It consists of a pair of interlocking identical gears, each coaxially holding a pressing wheel to intermittently compress plastic tubing in 0 - 10 mm length at every 1 cm interval. The performance of the processed tubing is examined in protein separation with 1.6 mm ID PTFE tubing intermittently pressed in 3 mm and 10 mm width both at 10 mm intervals at various flow rates and revolution speeds. A series of experiments was performed with a polymer phase system composed of polyethylene glycol and dibasic potassium phosphate each at 12.5% (w/w) in deionized water using three protein samples. Overall results clearly demonstrate that the compressed tubing can yield substantially higher peak resolution than the non-processed tubing. The simple tubing modifier is very useful for separation of proteins with high-speed countercurrent chromatography.
Laboratory of Bioseparation Technology, Biochemistry and Biophysics Center, National Heart, Lung, and Blood Institute, National Institutes of Health, 10 Center Drive, Bldg. 10, Room 8N230, Bethesda, MD 20892, USA.
Yoichiro
Y
Ito
Laboratory of Bioseparation Technology, Biochemistry and Biophysics Center, National Heart, Lung, and Blood Institute, National Institutes of Health, 10 Center Drive, Bldg. 10, Room 8N230, Bethesda, MD 20892, USA; College of Life Sciences, University of Chinese Academy of Sciences, No. 19A Yuquan Road, Beijing 100049, China.
Xiaofeng
X
Ma
Machine Instrumentation Design and Fabrication, National Institutes of Health, Bethesda, MD 20892, USA.
Robert
R
Clary
Intermittently pressed tubing; Polymer phase system; Protein separation; Simple tubing modifier; Spiral high-speed counter-current chromatography; Spiral tube assembly