Application study of 1,2-α-l-fucosynthase: introduction of Fucα1-2Gal disaccharide structures on N-glycan, ganglioside, and xyloglucan oligosaccharide.
We have recently generated a highly efficient 1,2-α-l-fucosynthase (BbAfcA N423H mutant) by protein engineering of 1,2-α-l-fucosidase from Bifidobacterium bifidum JCM 1254. This synthase could specifically introduce H-antigens (Fucα1-2Gal) into the non-reducing ends of oligosaccharides and in O-linked glycans in mucin glycoprotein. In the present study, we show an extended application of the engineered 1,2-α-l-fucosynthase by demonstrating its ability to insert Fuc residues into N- and O-glycans in fetuin glycoproteins, GM1 ganglioside, and a plant-derived xyloglucan nonasaccharide. This application study broadens the feasibility of this novel H-antigen synthesis technique in functional glycomics.
a Faculty of Bioresources and Environmental Sciences , Ishikawa Prefectural University , Nonoichi , Japan.
Yuta
Y
Sugiyama
a Faculty of Bioresources and Environmental Sciences , Ishikawa Prefectural University , Nonoichi , Japan.|b Graduate School of Biostudies , Kyoto University , Kyoto , Japan.
Toshihiko
T
Katoh
a Faculty of Bioresources and Environmental Sciences , Ishikawa Prefectural University , Nonoichi , Japan.
Yuji
Y
Honda
a Faculty of Bioresources and Environmental Sciences , Ishikawa Prefectural University , Nonoichi , Japan.|b Graduate School of Biostudies , Kyoto University , Kyoto , Japan.
Aina
A
Gotoh
c Faculty of Biology-Oriented Science and Technology , Kindai University , Kinokawa , Japan.
Hisashi
H
Ashida
a Faculty of Bioresources and Environmental Sciences , Ishikawa Prefectural University , Nonoichi , Japan.
Shin
S
Kurihara
a Faculty of Bioresources and Environmental Sciences , Ishikawa Prefectural University , Nonoichi , Japan.
Kenji
K
Yamamoto
a Faculty of Bioresources and Environmental Sciences , Ishikawa Prefectural University , Nonoichi , Japan.|b Graduate School of Biostudies , Kyoto University , Kyoto , Japan.
Takane
T
Katayama
D001212:Asialoglycoproteins / Q000378:metabolism; D001644:Bifidobacterium / Q000201:enzymology; D004187:Disaccharides / Q000737:chemistry* / Q000378:metabolism*; D060748:Fetuins / Q000378:metabolism; D005643:Fucose / Q000737:chemistry* / Q000378:metabolism; D005732:Gangliosides / Q000737:chemistry* / Q000378:metabolism; D005936:Glucans / Q000737:chemistry* / Q000378:metabolism; D006017:Glycolipids / Q000737:chemistry / Q000378:metabolism; D009154:Mutation; D009844:Oligosaccharides / Q000737:chemistry* / Q000378:metabolism; D010944:Plants / Q000737:chemistry; D015202:Protein Engineering; D014990:Xylans / Q000737:chemistry* / Q000378:metabolism; D005644:alpha-L-Fucosidase / Q000235:genetics / Q000378:metabolism*
D001212:Asialoglycoproteins; D004187:Disaccharides; D060748:Fetuins; D005732:Gangliosides; D005936:Glucans; D006017:Glycolipids; D009844:Oligosaccharides; D014990:Xylans; C017029:asialofetuin; D005643:Fucose; C029353:xyloglucan; D005644:alpha-L-Fucosidase
1,2-α-l-fucosidase; H-antigen; glycosynthase; oligosaccharide; sugar chains