Freeze-Dried Platelet-Rich Plasma Accelerates Bone Union with Adequate Rigidity in Posterolateral Lumbar Fusion Surgery Model in Rats.
Fresh platelet-rich plasma (PRP) accelerates bone union in rat model. However, fresh PRP has a short half-life. We suggested freeze-dried PRP (FD-PRP) prepared in advance and investigated its efficacy in vivo. Spinal posterolateral fusion was performed on 8-week-old male Sprague-Dawley rats divided into six groups based on the graft materials (n = 10 per group): sham control, artificial bone (A hydroxyapatite-collagen composite) -alone, autologous bone, artificial bone + fresh-PRP, artificial bone + FD-PRP preserved 8 weeks, and artificial bone + human recombinant bone morphogenetic protein 2 (BMP) as a positive control. At 4 and 8 weeks after the surgery, we investigated their bone union-related characteristics including amount of bone formation, histological characteristics of trabecular bone at remodeling site, and biomechanical strength on 3-point bending. Comparable radiological bone union was confirmed at 4 weeks after surgery in 80% of the FD-PRP groups, which was earlier than in other groups (p < 0.05). Histologically, the trabecular bone had thinner and more branches in the FD-PRP. Moreover, the biomechanical strength was comparable to that of autologous bone. FD-PRP accelerated bone union at a rate comparable to that of fresh PRP and BMP by remodeling the bone with thinner, more tangled, and rigid trabecular bone.
Department of Orthopaedic Surgery, Graduate School of Medicine, Chiba University, Chiba, Japan.
Yasuhiro
Y
Shiga
Department of Orthopaedic Surgery, Graduate School of Medicine, Chiba University, Chiba, Japan.
Sumihisa
S
Orita
Department of Orthopaedic Surgery, Graduate School of Medicine, Chiba University, Chiba, Japan.
Go
G
Kubota
Department of Orthopaedic Surgery, Graduate School of Medicine, Chiba University, Chiba, Japan.
Hiroto
H
Kamoda
Department of Orthopaedic Surgery, Graduate School of Medicine, Chiba University, Chiba, Japan.
Masaomi
M
Yamashita
Department of Orthopaedic Surgery, Graduate School of Medicine, Chiba University, Chiba, Japan.
Yusuke
Y
Matsuura
Department of Orthopaedic Surgery, Graduate School of Medicine, Chiba University, Chiba, Japan.
Kazuyo
K
Yamauchi
Department of Orthopaedic Surgery, Graduate School of Medicine, Chiba University, Chiba, Japan.
Yawara
Y
Eguchi
Department of Orthopaedic Surgery, Graduate School of Medicine, Chiba University, Chiba, Japan.
Miyako
M
Suzuki
Department of Orthopaedic Surgery, Graduate School of Medicine, Chiba University, Chiba, Japan.
Kazuhide
K
Inage
Department of Orthopaedic Surgery, Graduate School of Medicine, Chiba University, Chiba, Japan.
Takeshi
T
Sainoh
Department of Orthopaedic Surgery, Graduate School of Medicine, Chiba University, Chiba, Japan.
Jun
J
Sato
Department of Orthopaedic Surgery, Graduate School of Medicine, Chiba University, Chiba, Japan.
Kazuki
K
Fujimoto
Department of Orthopaedic Surgery, Graduate School of Medicine, Chiba University, Chiba, Japan.
Koki
K
Abe
Department of Orthopaedic Surgery, Graduate School of Medicine, Chiba University, Chiba, Japan.
Hirohito
H
Kanamoto
Department of Orthopaedic Surgery, Graduate School of Medicine, Chiba University, Chiba, Japan.
Masahiro
M
Inoue
Department of Orthopaedic Surgery, Graduate School of Medicine, Chiba University, Chiba, Japan.
Hideyuki
H
Kinoshita
Department of Orthopaedic Surgery, Eastern Chiba Medical Center, Chiba, Japan.
Yasuchika
Y
Aoki
Department of Orthopaedic Surgery, Showa University, Tokyo, Japan.
Tomoaki
T
Toyone
Department of Orthopaedic Surgery, Graduate School of Medicine, Chiba University, Chiba, Japan.
Takeo
T
Furuya
Department of Orthopaedic Surgery, Graduate School of Medicine, Chiba University, Chiba, Japan.
Masao
M
Koda
Department of Orthopaedic Surgery, Graduate School of Medicine, Chiba University, Chiba, Japan.
Kazuhisa
K
Takahashi
Department of Orthopaedic Surgery, Graduate School of Medicine, Chiba University, Chiba, Japan.
Seiji
S
Ohtori
D000818:Animals; D001861:Bone Regeneration; D000071556:Cancellous Bone / Q000166:cytology / Q000000981:diagnostic imaging / Q000601:surgery; D005612:Freeze Drying; D008159:Lumbar Vertebrae / Q000166:cytology / Q000000981:diagnostic imaging / Q000601:surgery*; D008297:Male; D053657:Platelet-Rich Plasma; D017207:Rats, Sprague-Dawley; D013123:Spinal Fusion / Q000379:methods