De novoTranscriptome Analysis Revealed Genes Involved in Flavonoid and Vitamin C Biosynthesis inPhyllanthus emblica(L.).
Phyllanthus emblicais an affluent source of various therapeutic components. A few of them like vitamin C and flavonoids are predominant bioactive compounds that are being used in immense pharmacological applications. In-spite of numerous applications, the genomic information of this plant was limited to a few expressed sequence tags (ESTs) in DNA databases. Herein, we developed in-depth transcriptome information ofP. emblicausing Illumina Hiseq 2000 platform and characterized. A total of 31,285,965 high-quality reads were assembled into 91,288 contigs with the N50 value 358. Out of them, 47,267 contigs were functionally annotated using BLASTX search against NCBI-non-redundant (NR) protein database. Further, 31,366 contigs showed similarity with various gene ontology (GO) terms, and 1299 were related to different enzymes and biosynthetic pathways. We identified the transcripts related to each gene involved in flavonoid and vitamin C biosynthesis. Severalcytochrome P450s(CYPs) andglucosyltransferases(GTs) genes involved in flavonoid biosynthesis and various other metabolic pathways were also documented. Further, 6510 transcription factors and 4420 EST derived simple sequence repeat (SSR) markers were also predicted. The present study enlightened various characteristic features ofP. emblicagenome, and provided an important resource for future molecular and functional genomics studies.
Department of Biotechnology, Panjab University Chandigarh, India.
Avneesh
A
Kumar
Department of Biotechnology, Panjab University Chandigarh, India.
Sunil
S
Kumar
Department of Biotechnology, Panjab University Chandigarh, India.
Savita
S
Bains
Department of Biotechnology, Panjab University Chandigarh, India.
Vanya
V
Vaidya
Department of Biotechnology, Panjab University Chandigarh, India.
Baljinder
B
Singh
Department of Biotechnology, Panjab University Chandigarh, India.
Ravneet
R
Kaur
Department of Biotechnology, Panjab University Chandigarh, India.
Jagdeep
J
Kaur
Department of Biotechnology, Panjab University Chandigarh, India.
Kashmir
K
Singh
Phyllanthus emblica; flavonoids; gene ontology; simple sequence repeats; transcription factors; transcriptome; vitamin C