Archetype and Rearranged Non-coding Control Regions in Urothelial Bladder Carcinoma of Immunocompetent Individuals.
BACKGROUND
Polyomaviruses (PyVs) are potential transforming viruses. Despite their involvement in human tumours still being debated, there is evidence to suggest a role for PyVs in bladder carcinoma (BC). Therefore, a possible association between PyVs and BC was investigated.


METHODS
Urine, blood and fresh bladder tissue specimens were collected from 29 patients with BC. PyV prevalence, non-coding control region (NCCR) organization and genotypic analysis were assessed.


RESULTS
Data showed a significant prevalence of John Cunningham (JC) PyV in BC tissues and in urine with respect to BKPyV, while simian virus 40 was not revealed. A BKPyV rearranged NCCR sequence was isolated, whereas a JCPyV archetypal structure was consistently retained. A prevalence of European genotypes was observed.


CONCLUSIONS
Our data would suggest a JCPyV involvement in cancer progression and a BKPyV association with BC pathogenesis in immunocompetent patients. However, further work is necessary to better understand the exact role of PyVs in urothelial carcinogenesis.
Department of Health Sciences and Infectious Diseases, Sapienza University of Rome, Rome, Italy.
Elena
E
Anzivino
Department of Health Sciences and Infectious Diseases, Sapienza University of Rome, Rome, Italy.
Maria Antonella
MA
Zingaropoli
Department of Health Sciences and Infectious Diseases, Sapienza University of Rome, Rome, Italy.
Marco
M
Iannetta
Department of Health Sciences and Infectious Diseases, Sapienza University of Rome, Rome, Italy.
Valeria Antonietta
VA
Pietropaolo
Department of Health Sciences and Infectious Diseases, Sapienza University of Rome, Rome, Italy.
Alessandra
A
Oliva
Department of Obstretics, Gynecology and Urological Sciences, Sapienza University of Rome, Rome, Italy.
Francesco
F
Iori
Department of Radiological Sciences, Oncology and Pathology, Sapienza University of Rome, Rome, Italy.
Antonio
A
Ciardi
Department of Health Sciences and Infectious Diseases, Sapienza University of Rome, Rome, Italy.
Donatella Maria
DM
Rodio
Department of Obstretics, Gynecology and Urological Sciences, Sapienza University of Rome, Rome, Italy.
Francesca
F
Antonini
Diagnostic Microbiology Service National Center for Microbiology, Majadahonda, Madrid, Spain.
Cesare Giovanni
CG
Fedele
Department of Health Sciences and Infectious Diseases, Sapienza University of Rome, Rome, Italy.
Alessandra
A
D'Abramo
Department of Health Sciences and Infectious Diseases, Sapienza University of Rome, Rome, Italy.
Claudio Maria
CM
Mastroianni
Department of Health Sciences and Infectious Diseases, Sapienza University of Rome, Rome, Italy.
Vincenzo
V
Vullo
Department of Health Sciences and Infectious Diseases, Sapienza University of Rome, Rome, Italy maria.ciardi@uniroma1.it.
Maria Rosa
MR
Ciardi
D000368:Aged; D000369:Aged, 80 and over; D001739:BK Virus / Q000235:genetics* / Q000302:isolation & purification / Q000472:pathogenicity; D005260:Female; D015321:Gene Rearrangement; D005838:Genotype; D006801:Humans; D007577:JC Virus / Q000235:genetics* / Q000302:isolation & purification / Q000472:pathogenicity; D008297:Male; D008875:Middle Aged; D022661:RNA, Untranslated / Q000235:genetics*; D001749:Urinary Bladder Neoplasms / Q000097:blood / Q000235:genetics* / Q000652:urine / Q000821:virology; D019459:Urothelium / Q000473:pathology / Q000821:virology
D022661:RNA, Untranslated
BKPyV; Polyomaviruses; PyVs; urothelial bladder carcinoma