Expression and functional role of Bone Morphogenetic Proteins (BMPs) in cyclical corpus luteum in buffalo (Bubalus bubalis).
The role of growth factors in the modulation of ovarian function is an interesting area of research in reproductive biology. Recently, we have shown the expression and role of IGF, EGF, VEGF and FGF in the follicle and CL. Here, we report the presence of Bone Morphogenetic Proteins (BMPs) and their functional receptors in the corpus luteum (CL) of buffalo. The bubaline CL was classified into four stages according to the morphology and progesterone (P4) concentration. The qPCR, immunoblot and immunohistochemistry studies revealed that BMP2 and BMP Receptors (BMPR1A, BMPR1B and BMPR2) were significantly upregulated during the mid stage whereas BMP4 and BMP7 were upregulated during the early stage of CL (P<0.05). Studies on primary luteal cell culture (LCC) using mid CL showed a significant time and concentration dependent effect of BMP4 and BMP7 (P<0.05). At 100ngml-1, the BMPs maximally stimulated the transcripts of StAR, CYP11A1 and 3βHSD that paralleled with P4accretion in the media (P<0.05). Further, the BMP4 as well as BMP7 upregulated the transcripts of PCNA and downregulated CASPASE3 in the LCC at the same concentration (P<0.05). Though the combined effect of BMP4 and 7 was significantly higher (P<0.05) than that of individual one, it was not additive. In conclusion, the expression of BMPs and their receptors were dependent on the stages of CL in the buffalo. Treatment of LCC with BMPs in vitro confirmed the presence of functional receptors that stimulated the P4production and luteal cell survival. Moreover, the results support the concept that the upregulation of P4and its biosynthetic pathway enzymes such as CYP11A1, StAR and 3βHSD in the CL is likely due to the autocrine and /or paracrine effects of BMP4 and BMP7 under physiological milieu.
Physiology & Climatology Division, Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243122, India.
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Rajesh
Physiology & Climatology Division, Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243122, India.
Avishek
A
Paul
Physiology & Climatology Division, Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243122, India.
S R
SR
Mishra
Physiology & Climatology Division, Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243122, India.
Jaya
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Bharati
Physiology & Climatology Division, Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243122, India.
Nipuna
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Thakur
Physiology & Climatology Division, Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243122, India.
Tanmay
T
Mondal
Physiology & Climatology Division, Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243122, India.
Sanjhali
S
Soren
Division of Pharmacology & Toxicology, Indian Veterinary Research Institute, Izatnagar 243122, India.
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S
Harikumar
Animal Reproduction Division, Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243122, India.
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K
Narayanan
Physiology & Climatology Division, Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243122, India.
V S
VS
Chouhan
Physiology & Climatology Division, Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243122, India.
Sadhan
S
Bag
Eastern Regional Station, Indian Veterinary Research Institute, Kolkatta 700037, India.
B C
BC
Das
Physiology & Climatology Division, Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243122, India.
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G
Singh
Physiology & Climatology Division, Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243122, India.
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VP
Maurya
Physiology & Climatology Division, Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243122, India.
G Taru
GT
Sharma
Physiology & Climatology Division, Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243122, India. Electronic address: msarkar24@gmail.com.
Mihir
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Sarkar
D000818:Animals; D017209:Apoptosis; D052004:Bone Morphogenetic Protein Receptors / Q000378:metabolism; D019485:Bone Morphogenetic Proteins / Q000235:genetics* / Q000378:metabolism; D002020:Buffaloes / Q000235:genetics*; D053148:Caspase 3 / Q000235:genetics / Q000378:metabolism; D002478:Cells, Cultured; D003338:Corpus Luteum / Q000378:metabolism*; D005260:Female; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D015151:Immunoblotting; D007150:Immunohistochemistry; D011374:Progesterone / Q000235:genetics / Q000378:metabolism; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D013997:Time Factors
D019485:Bone Morphogenetic Proteins; D018809:Proliferating Cell Nuclear Antigen; D012333:RNA, Messenger; D011374:Progesterone; D052004:Bone Morphogenetic Protein Receptors; D053148:Caspase 3
BMP; Buffalo; Corpus luteum; Progesterone