Preparation of a 3-kb Mate-Pair Library for Illumina Sequencing.
The "mate-pair" approach for dual end sequencing uses long library fragments (1000-10,000 bp) that are circularized by ligation to the ends of a single, common DNA adaptor of known sequence. Once the ends are mated to the adaptor, a final library of fragments that retain the adaptor and only the ends of the original, circularized piece of DNA can be obtained. This protocol describes how to prepare a 3-kb mate-paired-end library. The resulting amplified DNA is suitable for sequencing on the Illumina sequencer.
Elaine
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Mardis
W Richard
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McCombie
D004247:DNA / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification*; D015723:Gene Library; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods*; D017422:Sequence Analysis, DNA / Q000379:methods
D004247:DNA