Association between a non-synonymous HSD17B4 single nucleotide polymorphism and meat-quality traits in Berkshire pigs.
Single nucleotide polymorphisms (SNPs) are useful genetic markers that allow correlation of genetic sequences with phenotypic traits. It is shown here that HSD17B4, a bifunctional enzyme mediating dehydrogenation and anhydration during β-oxidation of long-chain fatty acids, contains a non-synonymous SNP (nsSNP) of chr2:128,825,976A>G, c.2137A>G, I690V, within the sterol carrier protein-2 domain of the HSD17B4 gene, by RNA-Seq of liver RNA. The HSD17B4 mRNA was highly expressed in the kidney and liver among various other tissues in four pig breeds, namely, Berkshire, Duroc, Landrace, and Yorkshire. The nsSNP was significantly associated with carcass weight, backfat thickness, and drip loss (P < 0.05). Furthermore, HSD17B4 may play a crucial role during the early stages of myogenesis when expression of its mRNA was significantly high. In conclusion, HSD17B4 may serve as a possible regulator of muscle development, and its identification should help to select for improved economic traits of Berkshire pigs such as carcass weight, backfat thickness, and drip loss.
Swine Science and Technology Center, Gyeongnam National University of Science & Technology, Jinju, South Korea.
J L
JL
Jo
Swine Science and Technology Center, Gyeongnam National University of Science & Technology, Jinju, South Korea.
J H
JH
Hwang
Swine Science and Technology Center, Gyeongnam National University of Science & Technology, Jinju, South Korea.
S G
SG
Kwon
Swine Science and Technology Center, Gyeongnam National University of Science & Technology, Jinju, South Korea.
D H
DH
Park
Swine Science and Technology Center, Gyeongnam National University of Science & Technology, Jinju, South Korea.
T W
TW
Kim
Swine Science and Technology Center, Gyeongnam National University of Science & Technology, Jinju, South Korea.
D G
DG
Kang
Swine Science and Technology Center, Gyeongnam National University of Science & Technology, Jinju, South Korea.
G E
GE
Yu
Department of Animal Resource Technology, Gyeongnam National University of Science & Technology, Jinju, South Korea.
I S
IS
Kim
Swine Science and Technology Center, Gyeongnam National University of Science & Technology, Jinju, South Korea jiha@gntech.ac.kr.
J G
JG
Ha
Swine Science and Technology Center, Gyeongnam National University of Science & Technology, Jinju, South Korea jiha@gntech.ac.kr.
C W
CW
Kim
D015067:17-Hydroxysteroid Dehydrogenases / Q000235:genetics*; D000818:Animals; D005260:Female; D015971:Gene Expression Regulation, Enzymologic; D056726:Genetic Association Studies; D008099:Liver / Q000201:enzymology; D008297:Male; D008460:Meat / Q000592:standards*; D051379:Mice; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D019655:Quantitative Trait, Heritable; D060888:Real-Time Polymerase Chain Reaction; D034421:Sus scrofa / Q000235:genetics*
D015067:17-Hydroxysteroid Dehydrogenases