Decreased paraoxonase1 activity and increased malondialdehyde and oxidative DNA damage levels in primary open angle glaucoma.
To investigate the malondialdehyde (MDA) levels, paraoxonase1 (PON1) activity and 8-hydroxy 2-deoxyguanosine (8-OHdG) levels in the primary open angle glaucoma (POAG) patient. Blood samples from 52 healthy individuals and 53 patients with POAG were analyzed for MDA and 8-OHdG by HPLC (high-performance liquid chromatography) and PON1 by spectrophotometry. The data obtained were analyzed statistically. MDA levels were 10.46±8.4 and 4.70±1.79 µmol; PON1 levels were 121±39.55 and 161.62±60.22 U/mL; and 8-OHdG values were 1.32±0.53/106dG and 0.47±0.27/106dG in the POAG patients and the control group, respectively. The difference was significant in MDA levels, 8-OHdG levels and PON1 activity in POAG patients in comparison with controls (P<0.001). We concluded that the observed increase in MDA and 8-OHdG levels may be correlated with decreased PON1 activity. Oxidative stress plays an important role in glaucoma development.
Department of Ophthalmology, Haydarpasa Training and Research Hospital, Istanbul 34668, Turkey.
Ugur Yilmaz
UY
Mumcu
Department of Ophthalmology, Faculty of Medicine, Atatürk University, Erzurum 25040, Turkey.
Ibrahim
I
Kocer
Department of Ophthalmology, Faculty of Medicine, Atatürk University, Erzurum 25040, Turkey.
Orhan
O
Ates
Department of Biochemistry, Atatürk University, Erzurum 25040, Turkey.
H Hakan
HH
Alp
8-hydroxy 2-deoxyguanosine; glaucoma; paraoxonase