Changes in morphology of actin filaments and expression of alkaline phosphatase at 3D cultivation of MG-63 osteoblast-like cells on mineralized fibroin scaffolds.
3D cultivation of MG-63 osteoblast-like cells on mineralized fibroin scaffolds leads to an increase in the expression of alkaline phosphatase, an early marker of bone formation. Increased expression is associated with the actin cytoskeleton reorganization under the influence of 3D cultivation and osteogenic calcium phosphate component of the microcarrier.
Faculty of Biology, Moscow State University, Moscow, 119234, Russia. pylaevanna@gmail.com.
A V
AV
Goncharenko
Faculty of Biology, Moscow State University, Moscow, 119234, Russia.
N V
NV
Malyuchenko
Faculty of Biology, Moscow State University, Moscow, 119234, Russia.
A M
AM
Moisenovich
Faculty of Biology, Moscow State University, Moscow, 119234, Russia.
M S
MS
Kotlyarova
Faculty of Biology, Moscow State University, Moscow, 119234, Russia.
A Yu
AY
Arkhipova
Faculty of Biology, Moscow State University, Moscow, 119234, Russia.
A S
AS
Kon'kov
Shumakov Research Institute of Transplantation and Artificial Organs, Russian Ministry of Health, ul. Shchukinskaya 1, Moscow, 113182, Russia.
I I
II
Agapov
Vladimirskii Moscow Regional Research Clinical Institute, ul. Shchepkina 61/2, Moscow, 129110, Russia.
A V
AV
Molochkov
Faculty of Biology, Moscow State University, Moscow, 119234, Russia.
M M
MM
Moisenovich
Faculty of Biology, Moscow State University, Moscow, 119234, Russia.
M P
MP
Kirpichnikov
D008841:Actin Cytoskeleton / Q000378:metabolism*; D000469:Alkaline Phosphatase / Q000378:metabolism*; D001861:Bone Regeneration; D018786:Bone Substitutes; D002130:Calcium Phosphates / Q000378:metabolism; D002448:Cell Adhesion; D018929:Cell Culture Techniques; D002460:Cell Line; D005349:Fibroins; D005780:Gelatin; D005898:Glass; D006801:Humans; D010006:Osteoblasts / Q000166:cytology* / Q000378:metabolism*; D010012:Osteogenesis / Q000502:physiology; D016062:Porosity; D054457:Tissue Scaffolds
D018786:Bone Substitutes; D002130:Calcium Phosphates; D005780:Gelatin; D005349:Fibroins; C020243:calcium phosphate; D000469:Alkaline Phosphatase