DNA Binding Peptide Directed Synthesis of Continuous DNA Nanowires for Analysis of Large DNA Molecules by Scanning Electron Microscope.
Synthesis of smooth and continuous DNA nanowires, preserving the original structure of native DNA, and allowing its analysis by scanning electron microscope (SEM), is demonstrated. Gold nanoparticles densely assembled on the DNA backbone via thiol-tagged DNA binding peptides work as seeds for metallization of DNA. This method allows whole analysis of DNA molecules with entangled 3D features.
School of Advanced Materials Science and Engineering, Sungkyunkwan University (SKKU), Suwon, 16419, Republic of Korea.
Kyung-Il
KI
Kim
Department of Chemistry and Interdisciplinary Program of Integrated Biotechnology, Sogang University, Seoul, 04107, Republic of Korea.
Seonghyun
S
Lee
Department of Chemistry and Interdisciplinary Program of Integrated Biotechnology, Sogang University, Seoul, 04107, Republic of Korea.
Xuelin
X
Jin
SKKU Advanced Institute of Nanotechnology (SAINT), Sungkyunkwan University (SKKU), Suwon, 16419, Republic of Korea.
Su Ji
SJ
Kim
Department of Chemistry and Interdisciplinary Program of Integrated Biotechnology, Sogang University, Seoul, 04107, Republic of Korea.
Kyubong
K
Jo
School of Advanced Materials Science and Engineering, Sungkyunkwan University (SKKU), Suwon, 16419, Republic of Korea.|SKKU Advanced Institute of Nanotechnology (SAINT), Sungkyunkwan University (SKKU), Suwon, 16419, Republic of Korea.
Jung Heon
JH
Lee
D000595:Amino Acid Sequence; D004247:DNA / Q000032:analysis*; D006046:Gold / Q000737:chemistry; D008855:Microscopy, Electron, Scanning / Q000295:instrumentation*; D053770:Nanowires / Q000737:chemistry* / Q000648:ultrastructure; D010455:Peptides / Q000737:chemistry / Q000378:metabolism*; D013438:Sulfhydryl Compounds / Q000737:chemistry
D010455:Peptides; D013438:Sulfhydryl Compounds; D006046:Gold; D004247:DNA
DNA analysis; DNA binding peptides; DNA nanowires; continuous metallization; scanning electron microscope (SEM)