Circulation autoantibody against Lamin A/C in patients with Sjögren's syndrome.
Lamin A/C proteins are major components of nuclear laminae and were encoded by the LMNA gene. Recent studies have found that in addition to provides nuclear-membrane strength; it also regulates the gene expression. Lamin A/C has been confirmed as an autoantigen in RA, SLE and vasculitis. Anti-Lamin A/C antibodies also have been found by indirect immunofluorescence method. In this study, we used various research methods to confirm Lamin A/C is an autoantigen in Han Chinese patients with confirmed Sjögren's syndrome (SS). To further investigate the relationship between the autoimmune disease antigens, we compared the amino acid sequence of Lamin A/C epitope and several common antigens' antigenic determinant. As a result, we found that Lamin A/C has similar epitopes with U1RNP. It means that the potential relationship exist between Lamin A/C and U1RNP. Clinical data we collected also showed that anti-Lamin A/C and anti-U1RNP antibodies always appear in same serum sample. Therefore, we speculated that cross-reaction may take place between antigen and potential antigen, which have similar epitope. Then, by epitope spreading, the potential antigen can be a new autoantigen. Our study provided a new thinking for further research about the relationship between autoantigens and their development mechanism in autoimmune diseases.
112 Laboratory, School of Chemistry and Biological Engineering, University of Science and Technology Beijing, Beijing, China.
Wen
W
Zhang
Department of Clinical Biochemistry, Chinese PLA General Hospital, Beijing, China.
Chunyan
C
Zhang
112 Laboratory, School of Chemistry and Biological Engineering, University of Science and Technology Beijing, Beijing, China.
Peng
P
Chen
112 Laboratory, School of Chemistry and Biological Engineering, University of Science and Technology Beijing, Beijing, China.
Chunhe
C
Yang
112 Laboratory, School of Chemistry and Biological Engineering, University of Science and Technology Beijing, Beijing, China.
Xianfeng
X
Gan
112 Laboratory, School of Chemistry and Biological Engineering, University of Science and Technology Beijing, Beijing, China.
Muhammad
M
Hussain
112 Laboratory, School of Chemistry and Biological Engineering, University of Science and Technology Beijing, Beijing, China.
Yiping
Y
Xun
Department of Clinical Biochemistry, Chinese PLA General Hospital, Beijing, China.
Yaping
Y
Tian
112 Laboratory, School of Chemistry and Biological Engineering, University of Science and Technology Beijing, Beijing, China.
Hongwu
H
Du
D000328:Adult; D000368:Aged; D001323:Autoantibodies / Q000097:blood*; D015415:Biomarkers / Q000097:blood; D016022:Case-Control Studies; D002681:China; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes; D005260:Female; D006801:Humans; D034904:Lamin Type A / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D017412:Ribonucleoprotein, U1 Small Nuclear / Q000276:immunology; D012859:Sjogren's Syndrome / Q000097:blood* / Q000175:diagnosis / Q000276:immunology; D055815:Young Adult
D001323:Autoantibodies; D015415:Biomarkers; D000939:Epitopes; C494847:LMNA protein, human; D034904:Lamin Type A; D017412:Ribonucleoprotein, U1 Small Nuclear
Lamin A/C; Pathology Section; Sjögren’s syndrome; autoantigen; autoimmune diseases; similar antigen epitopes