Primer containing dimethylaminododecyl methacrylate kills bacteria impregnated in human dentin blocks.
Antibacterial dimethylaminododecyl methacrylate (DMADDM) was recently synthesized. The objectives of this study were to: (1) investigate antibacterial activity of DMADDM-containing primer on Streptococcus mutans impregnated into dentin blocks for the first time, and (2) compare the antibacterial efficacy of DMADDM with a previous quaternary ammonium dimethacrylate (QADM). Scotchbond Multi-Purpose (SBMP) bonding agent was used. DMADDM and QADM were mixed into SBMP primer. Six primers were tested: SBMP control primer P, P+2.5% DMADDM, P+5% DMADDM, P+7.5% DMADDM, P+10% DMADDM, and P+10% QADM. S. mutans were impregnated into human dentin blocks, and each primer was applied to dentin to test its ability to kill bacteria in dentinal tubules. Bacteria in dentin were collected via a sonication method, and the colony-forming units (CFU) and inhibition zones were measured. The bacterial inhibition zone of P+10% DMADDM was 10 times that of control primer (P<0.05). CFU in dentin with P+10% DMADDM was reduced by three orders of magnitude, compared with control. DMADDM had a much stronger antibacterial effect than QADM, and antibacterial efficacy increased with increasing DMADDM concentration. Dentin shear bond strengths were similar among all groups (P>0.1). In conclusion, antibacterial DMADDM-containing primer was validated to kill bacteria inside dentin blocks, possessing a much stronger antibacterial potency than the previous QADM. DMADDM-containing bonding agent was effective in eradicating bacteria in dentin, and its efficacy was directly proportional to DMADDM mass fraction. Therefore, DMADDM may be promising for use in bonding agents as well as in other restorative and preventive materials to inhibit bacteria.
State Key Laboratory of Oral Diseases, West China School of Stomatology, Sichuan University, Chengdu, China.|Biomaterials & Tissue Engineering Division, Department of Endodontics, Periodontics and Prosthodontics, University of Maryland Dental School, Baltimore, USA.
Chen
C
Chen
State Key Laboratory of Oral Diseases, West China School of Stomatology, Sichuan University, Chengdu, China.
Lei
L
Cheng
Biomaterials & Tissue Engineering Division, Department of Endodontics, Periodontics and Prosthodontics, University of Maryland Dental School, Baltimore, USA.
Michael D
MD
Weir
Biomaterials Group, Biosystems and Biomaterials Division, National Institute of Standards & Technology, Gaithersburg, USA.
Nancy J
NJ
Lin
Biomaterials Group, Biosystems and Biomaterials Division, National Institute of Standards & Technology, Gaithersburg, USA.
Sheng
S
Lin-Gibson
State Key Laboratory of Oral Diseases, West China School of Stomatology, Sichuan University, Chengdu, China.
Xue-Dong
XD
Zhou
Biomaterials & Tissue Engineering Division, Department of Endodontics, Periodontics and Prosthodontics, University of Maryland Dental School, Baltimore, USA.|Center for Stem Cell Biology & Regenerative Medicine, University of Maryland School of Medicine, Baltimore, USA.|Department of Mechanical Engineering, University of Maryland, Baltimore County, USA.
Hockin Hk
HH
Xu
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D018441:Biofilms; D003804:Dentin / Q000737:chemistry*; D017220:Dentin-Bonding Agents; D006801:Humans; D008422:Materials Testing; D008689:Methacrylates / Q000494:pharmacology*; D000644:Quaternary Ammonium Compounds / Q000494:pharmacology*; D019279:Resin Cements; D013295:Streptococcus mutans
D000900:Anti-Bacterial Agents; D017220:Dentin-Bonding Agents; D008689:Methacrylates; D000644:Quaternary Ammonium Compounds; D019279:Resin Cements; C084018:Scotchbond Multi-Purpose; C586974:dimethylaminododecyl methacrylate