Altered hippocampal arteriole structure and function in a rat model of preeclampsia: Potential role in impaired seizure-induced hyperemia.
We investigated the effect of experimental preeclampsia on hyperemia during seizure in the hippocampus and vascular function and structure of hippocampal arterioles using Sprague Dawley rats (n = 14/group) that were nonpregnant, pregnant (d20), or had experimental preeclampsia (induced by a high cholesterol diet d7-20). Hyperemia was measured via hydrogen clearance basally and during pentylenetetrazol-induced seizure (40-130 mg/kg i.v.). Reactivity of isolated and pressurized hippocampal arterioles to KCl, nitric oxide synthase inhibition with NG-nitro-L-arginine methyl ester and the nitric oxide donor sodium nitroprusside were investigated. Capillary density was quantified via immunohistochemistry. Cerebral blood flow increased during seizure vs. baseline in pregnant (118 ± 14 vs. 87 ± 9 mL/100 g/min; p < 0.05) and nonpregnant rats (106 ± 9 vs. 82 ± 9 mL/100 g/min; p < 0.05) but was unchanged in preeclamptic rats (79 ± 16 vs. 91 ± 4 mL/100 g/min; p > 0.05), suggesting impaired seizure-induced hyperemia in preeclampsia. Hippocampal arterioles from preeclamptic rats had less basal tone, and dilated less to 15 mM KCl (9 ± 8%) vs. pregnant (61 ± 27%) and nonpregnant rats (20 ± 11%). L-NAME had no effect on hippocampal arterioles in any group, but dilation to sodium nitroprusside was similar. Structurally, hippocampal arterioles from preeclamptic rats underwent inward hypotrophic remodeling and capillary rarefaction. Impaired seizure-induced hyperemia, vascular dysfunction, and limited vasodilatory reserve of hippocampal arterioles could potentiate hippocampal injury in preeclampsia especially during eclampsia.
1 Department of Neurological Sciences, University of Vermont College of Medicine, Burlington, USA.
Abbie C
AC
Johnson
1 Department of Neurological Sciences, University of Vermont College of Medicine, Burlington, USA.|2 Department of Obstetrics, Gynecology, and Reproductive Sciences, University of Vermont College of Medicine, Burlington, USA.|3 Department of Pharmacology, University of Vermont College of Medicine, Burlington, USA.
Marilyn J
MJ
Cipolla
D000818:Animals; D001160:Arterioles / Q000473:pathology* / Q000503:physiopathology; D056654:CA3 Region, Hippocampal / Q000098:blood supply* / Q000503:physiopathology; D002560:Cerebrovascular Circulation / Q000502:physiology*; D005260:Female; D006940:Hyperemia / Q000150:complications / Q000473:pathology* / Q000503:physiopathology; D011225:Pre-Eclampsia / Q000473:pathology* / Q000503:physiopathology; D011247:Pregnancy; D017207:Rats, Sprague-Dawley; D012640:Seizures / Q000150:complications / Q000473:pathology* / Q000503:physiopathology
Cerebral blood flow; cerebral vascular function; hippocampus; preeclampsia/eclampsia; seizure