Impaired telomere length and telomerase activity in peripheral blood leukocytes and granulosa cells in patients with biochemical primary ovarian insufficiency.
Are telomere length and telomerase activity associated with biochemical primary ovarian insufficiency (POI)?



Shortened telomere length and diminished telomerase activity were associated with biochemical POI.



POI is a result of pathological reproductive aging and encompasses occult, biochemical and overt stages. Studies have indicated telomere length as a biomarker for biological aging.



A total of 120 patients with biochemical POI and 279 control women were recruited by the Center for Reproductive Medicine of Shandong University.



Telomere length in peripheral blood leukocytes (LTL) and granulosa cells (GTL) was measured using a modified Quantitative Polymerase Chain Reaction technique. The relative telomerase activity (RTA) in granulosa cells was detected using a modified quantitative-telomeric repeat amplification protocol assay.



After adjusting for age, patients with biochemical POI (n = 120) exhibited significantly shorter LTLs (0.75 ± 0.09 vs 1.79 ± 0.12, P < 0.001; OR = 0.54, 95% CI = 0.43-0.68) and GTLs (0.78 ± 0.09 vs 1.90 ± 0.23, P < 0.001; OR = 0.54, 95% CI = 0.41-0.70) than the controls (n = 279 for LTLs; n = 90 for GTLs). Significantly diminished RTAs in granulosa cells were detected in patients with biochemical POI (n = 31) compared with the controls (n = 38) (1.57 ± 0.59 vs 4.63 ± 0.93, P = 0.025; OR = 0.84, 95% CI = 0.72-0.98).



N/A.



The cross-sectional nature of this study might have its limit in telomere length as well as telomerase activity along with the progressing decline in ovarian function.



These findings suggest that telomere length and telomerase activity may be considered as indicators for progression of ovarian decline.



This research was supported by the National Basic Research Program of China (973 Program) (2012CB944700), Science research foundation item of no-earnings health vocation (201402004) and the National Natural Science Foundation of China (31471352, 81270662, 81471509, 81300461, 81522018). The authors have no potential conflict of interest to declare.
Center for Reproductive Medicine, Provincial Hospital Affiliated to Shandong University, No. 324 Jingwu Road, Jinan 250021, P.R. China.|National Research Center for Assisted Reproductive Technology and Reproductive Genetics, Jinan 250021, P.R. China.|The Key laboratory of Reproductive Endocrinology (Shandong University) Jinan, P.R. China.
Xiaofei
X
Xu
Center for Reproductive Medicine, Provincial Hospital Affiliated to Shandong University, No. 324 Jingwu Road, Jinan 250021, P.R. China.|National Research Center for Assisted Reproductive Technology and Reproductive Genetics, Jinan 250021, P.R. China.|The Key laboratory of Reproductive Endocrinology (Shandong University) Jinan, P.R. China.
Xinxia
X
Chen
Center for Reproductive Medicine, Provincial Hospital Affiliated to Shandong University, No. 324 Jingwu Road, Jinan 250021, P.R. China.|National Research Center for Assisted Reproductive Technology and Reproductive Genetics, Jinan 250021, P.R. China.|The Key laboratory of Reproductive Endocrinology (Shandong University) Jinan, P.R. China.
Xiruo
X
Zhang
State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, P.R. China.
Yixun
Y
Liu
Center for Reproductive Medicine, Provincial Hospital Affiliated to Shandong University, No. 324 Jingwu Road, Jinan 250021, P.R. China.|National Research Center for Assisted Reproductive Technology and Reproductive Genetics, Jinan 250021, P.R. China.|The Key laboratory of Reproductive Endocrinology (Shandong University) Jinan, P.R. China.
Zhao
Z
Wang
Center for Reproductive Medicine, Provincial Hospital Affiliated to Shandong University, No. 324 Jingwu Road, Jinan 250021, P.R. China.|National Research Center for Assisted Reproductive Technology and Reproductive Genetics, Jinan 250021, P.R. China.|The Key laboratory of Reproductive Endocrinology (Shandong University) Jinan, P.R. China.
Peng
P
Wang
Shanghai Key Laboratory for Assisted Reproduction and Reproductive Genetics, Shanghai, P.R. China.
Yanzhi
Y
Du
Center for Reproductive Medicine, Provincial Hospital Affiliated to Shandong University, No. 324 Jingwu Road, Jinan 250021, P.R. China.|National Research Center for Assisted Reproductive Technology and Reproductive Genetics, Jinan 250021, P.R. China.|The Key laboratory of Reproductive Endocrinology (Shandong University) Jinan, P.R. China.
Yingying
Y
Qin
Center for Reproductive Medicine, Provincial Hospital Affiliated to Shandong University, No. 324 Jingwu Road, Jinan 250021, P.R. China chenzijiang@hotmail.com.|National Research Center for Assisted Reproductive Technology and Reproductive Genetics, Jinan 250021, P.R. China.|The Key laboratory of Reproductive Endocrinology (Shandong University) Jinan, P.R. China.|Shanghai Key Laboratory for Assisted Reproduction and Reproductive Genetics, Shanghai, P.R. China.|Center for Reproductive Medicine, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200030, P.R. China.
Zi-Jiang
ZJ
Chen
D000328:Adult; D003430:Cross-Sectional Studies; D005260:Female; D006107:Granulosa Cells / Q000378:metabolism*; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000378:metabolism*; D016649:Primary Ovarian Insufficiency / Q000378:metabolism*; D019098:Telomerase / Q000378:metabolism*; D016615:Telomere / Q000378:metabolism*
D019098:Telomerase
granulosa cell; leukocyte; primary ovarian insufficiency; telomerase activity; telomere length