Simultaneous determination of chlortetracycline, ampicillin and sarafloxacin in milk using capillary electrophoresis with electrochemiluminescence detection.
A fast, inexpensive and sensitive approach for the simultaneous determination of chlortetracycline, ampicilline and sarafloxacin in milk was developed using capillary electrophoresis coupled with an electrochemiluminescence detector. Under the optimal detection conditions, the linear ranges for chlortetracyline, ampicilline and sarafloxacin were 0.030-5.0, 0.050-5.0 and 0.0040-2.0 μg ml-1, respectively. The correlation coefficients of chlortetracycline, ampicilline and sarafloxacin were determined as 0.9997, 0.9952 and 0.9978, respectively. Detection limits (S/N = 3) of chlortetracycline, ampicilline and sarafloxacin were found as 0.017, 0.018 and 0.0013 μg ml-1, respectively. This method was successfully applied for the determination of chlortetracycline, ampicilline and sarafloxacin in milk. The recoveries were between 95.3% and 100%. The relative standard deviations of the detection limit and recovery were less than 2.6% and 3.2%, respectively.
a Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources (Ministry of Education of China), School of Chemistry and Pharmaceutical Sciences , Guangxi Normal University , Guilin , China.
Chanjuan
C
Long
a Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources (Ministry of Education of China), School of Chemistry and Pharmaceutical Sciences , Guangxi Normal University , Guilin , China.
Biyang
B
Deng
a Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources (Ministry of Education of China), School of Chemistry and Pharmaceutical Sciences , Guangxi Normal University , Guilin , China.
Shuangjiao
S
Sun
a Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources (Ministry of Education of China), School of Chemistry and Pharmaceutical Sciences , Guangxi Normal University , Guilin , China.
Sa
S
Meng
D000667:Ampicillin / Q000032:analysis*; D000818:Animals; D002751:Chlortetracycline / Q000032:analysis*; D002939:Ciprofloxacin / Q000031:analogs & derivatives* / Q000032:analysis; D055664:Electrochemical Techniques / Q000379:methods; D019075:Electrophoresis, Capillary / Q000379:methods*; D057230:Limit of Detection; D008163:Luminescent Measurements; D008892:Milk / Q000737:chemistry*; D015203:Reproducibility of Results
D002939:Ciprofloxacin; D000667:Ampicillin; C047224:sarafloxacin; D002751:Chlortetracycline
Capillary electrophoresis; antibiotic; electrochemiluminescence; milk; residue