Use of Adipose-Derived Stem Cells to Support Topical Skin Adhesive for Wound Closure: A Preliminary Report from Animal In Vivo Study.
The aim of this study was to determine the local and systemic effects of adipose-derived stem cells (ADSCs) as a component of topical skin adhesive in an animal artificial wound closure model. In presented study the cosmetic effects, histological analysis, mechanical properties, and cell migration have been assessed to evaluate the usefulness of ADSCs as supporting factor for octyl blend cyanoacrylate adhesive. The total of 40 rats were used and divided into six groups. In the Study Group, ADSCs were administered by multipoint injection of the six surrounding intrawound areas with additional freely leaving procedure of the cells between the skin flaps just before applying adhesive to close the wound. Five control groups without using ADSCs, utilizing different types of standard wound closure, were created in order to check efficiency of experimental stem cell therapy. In our study, we proved that ADSCs could be used effectively also as a supportive tool in topical skin adhesive for wound closure. However we did not achieve any spectacular differences related to such aspects as better mechanical properties or special biological breakthroughs in wound healing properties. The use of stem cells, especially ADSCs for wound closure can provide an inspiring development in plastic and dermatologic surgery.
Chair and Department of Surgical Oncology, Ludwik Rydygier Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University in Torun, Oncology Centre-Prof. Franciszek Łukaszczyk Memorial Hospital in Bydgoszcz, ul. Romanowskiej 2, 85-796 Bydgoszcz, Poland.
Maciej
0000-0001-8424-7198
M
Nowacki
Chair of Cosmetology and Aesthetic Dermatology, Ludwik Rydygier Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University in Torun, ul. Jagiellońska 15, 85-067 Bydgoszcz, Poland; Chair and Department of Rehabilitation, Ludwik Rydygier Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University in Torun, ul. M. Curie Skłodowskiej 9, 85-094 Bydgoszcz, Poland.
Katarzyna
K
Pietkun
Department of Plastic, Reconstructive and Aesthetic Surgery, Ludwik Rydygier Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University in Torun, ul. M. Curie Skłodowskiej 9, 85-094 Bydgoszcz, Poland; Chair of Urology, Department of Regenerative Medicine, Ludwik Rydygier Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University in Torun, ul. M. Curie Skłodowskiej 9, 85-094 Bydgoszcz, Poland.
Arkadiusz
A
Jundziłł
Chair of Urology, Department of Regenerative Medicine, Ludwik Rydygier Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University in Torun, ul. M. Curie Skłodowskiej 9, 85-094 Bydgoszcz, Poland.
Tomasz
0000-0001-5599-9315
T
Kloskowski
Department and Clinic of Dermatology, Sexually Transmitted Diseases and Immunodermatology, Ludwik Rydygier Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University in Torun, ul. M. Curie Skłodowskiej 9, 85-094 Bydgoszcz, Poland.
Dariusz
D
Grzanka
Department of Chemistry of Biomaterials and Cosmetics, Faculty of Chemistry, Nicolaus Copernicus University in Torun, Gagarina 7, 87-100 Torun, Poland.
Joanna
J
Skopinska-Wisniewska
Department and Clinic of Dermatology, Sexually Transmitted Diseases and Immunodermatology, Ludwik Rydygier Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University in Torun, ul. M. Curie Skłodowskiej 9, 85-094 Bydgoszcz, Poland.
Kinga
K
Scibior
Department of Histology and Embryology, Ludwik Rydygier Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University in Torun, ul. Karłowicza 24, 85-092 Bydgoszcz, Poland.
Maciej
0000-0002-5445-8821
M
Gagat
Chair of Urology, Department of Regenerative Medicine, Ludwik Rydygier Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University in Torun, ul. M. Curie Skłodowskiej 9, 85-094 Bydgoszcz, Poland.
Marta
M
Pokrywczyńska
Department of Histology and Embryology, Ludwik Rydygier Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University in Torun, ul. Karłowicza 24, 85-092 Bydgoszcz, Poland.
Alina
A
Grzanka
Chair and Department of Surgical Oncology, Ludwik Rydygier Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University in Torun, Oncology Centre-Prof. Franciszek Łukaszczyk Memorial Hospital in Bydgoszcz, ul. Romanowskiej 2, 85-796 Bydgoszcz, Poland.
Wojciech
W
Zegarski
Department and Clinic of Dermatology, Sexually Transmitted Diseases and Immunodermatology, Ludwik Rydygier Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University in Torun, ul. M. Curie Skłodowskiej 9, 85-094 Bydgoszcz, Poland.
Rafał
0000-0003-3418-1252
R
Czajkowski
Chair of Urology, Department of Regenerative Medicine, Ludwik Rydygier Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University in Torun, ul. M. Curie Skłodowskiej 9, 85-094 Bydgoszcz, Poland.
Tomasz
0000-0001-5347-4136
T
Drewa
Chair of Cosmetology and Aesthetic Dermatology, Ludwik Rydygier Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University in Torun, ul. Jagiellońska 15, 85-067 Bydgoszcz, Poland.
Barbara
0000-0001-9259-2637
B
Zegarska
D000273:Adipose Tissue; D000287:Administration, Topical; D000818:Animals; D051381:Rats; D011923:Rats, Nude; D013234:Stem Cells; D014014:Tissue Adhesives / Q000737:chemistry / Q000494:pharmacology*; D058106:Wound Closure Techniques; D014947:Wounds and Injuries / Q000628:therapy*
D014014:Tissue Adhesives