Archaeal orthologs of Cdc45 and GINS form a stable complex that stimulates the helicase activity of MCM.
The regulated recruitment of Cdc45 and GINS is key to activating the eukaryotic MCM(2-7) replicative helicase. We demonstrate that the homohexameric archaeal MCM helicase associates with orthologs of GINS and Cdc45 in vivo and in vitro. Association of these factors with MCM robustly stimulates the MCM helicase activity. In contrast to the situation in eukaryotes, archaeal Cdc45 and GINS form an extremely stable complex before binding MCM. Further, the archaeal GINS•Cdc45 complex contains two copies of Cdc45. Our analyses give insight into the function and evolution of the conserved core of the archaeal/eukaryotic replisome.
Molecular and Cellular Biochemistry Department, Indiana University, Bloomington, IN 47405.|Biology Department, Indiana University, Bloomington, IN 47405.
Yuli
Y
Xu
Sir William Dunn School of Pathology, Oxford OX13RE, United Kingdom.
Tamzin
T
Gristwood
Sir William Dunn School of Pathology, Oxford OX13RE, United Kingdom.
Ben
B
Hodgson
Chemistry Department, Indiana University, Bloomington, IN 47405.
Jonathan C
JC
Trinidad
Max Planck Institute für terrestrische Mikrobiologie, D-35043 Marburg, Germany.
Sonja-Verena
SV
Albers
Molecular and Cellular Biochemistry Department, Indiana University, Bloomington, IN 47405; stedbell@indiana.edu.|Biology Department, Indiana University, Bloomington, IN 47405.
Stephen D
SD
Bell
D001105:Archaea / Q000378:metabolism*; D019843:Archaeal Proteins / Q000378:metabolism; D018797:Cell Cycle Proteins / Q000378:metabolism*; D002868:Chromosomal Proteins, Non-Histone / Q000378:metabolism*; D019143:Evolution, Molecular; D064110:Minichromosome Maintenance Proteins / Q000378:metabolism*; D046912:Multiprotein Complexes / Q000737:chemistry / Q000378:metabolism; D011485:Protein Binding
D019843:Archaeal Proteins; D018797:Cell Cycle Proteins; D002868:Chromosomal Proteins, Non-Histone; D046912:Multiprotein Complexes; D064110:Minichromosome Maintenance Proteins
Archaea; CMG; DNA replication; MCM; helicase