Polysaccharide of an edible truffle Tuber rufum: Structural studies and effects on human lymphocytes.
A water-soluble heteroglycan (PS-II) with average molecular weight ∼7.27×104Da, was isolated from the fruiting bodies of an edible truffle mushroom Tuber rufum (Pico) var. by hot water extraction. The structural investigation of PS-II has been carried out using acid hydrolysis, methylation analysis, periodate oxidation, and 1D/2D NMR experiments. It was composed of d-glucose, d-galactose, l-fucose in a molar ratio of nearly 4:3:1 respectively. On the basis of these experiments, the repeating unit of the PS-II was found to contain a backbone of two (1→6)-α-d-galactopyranosyl, one (1→4)-α-d-glucopyranosyl, two (1→6)-β-d-glucopyranosyl, and one (1→4)-β-d-glucopyranosyl residues, out of which (1→4)-α-d-glucopyranosyl residue was branched at O-2 position with terminal α-l-fucopyranosyl residue and at O-6 position with terminal α-d-galactopyranosyl residue. Ameliorative activities of the PS-II was observed at different concentrations (25, 50, 100, 200, 400μg/ml) and it maintained the redox balance as well as reduced the lipid peroxidation to protect the cell damage.
Department of Chemistry and Chemical Technology, Vidyasagar University, Midnapore 721102, West Bengal, India.
Manabendra
M
Pattanayak
Department of Chemistry and Chemical Technology, Vidyasagar University, Midnapore 721102, West Bengal, India.
Surajit
S
Samanta
Department of Chemistry and Chemical Technology, Vidyasagar University, Midnapore 721102, West Bengal, India.
Prasenjit
P
Maity
Department of Chemistry and Chemical Technology, Vidyasagar University, Midnapore 721102, West Bengal, India.
Dilip K
DK
Manna
Department of Chemistry and Chemical Technology, Vidyasagar University, Midnapore 721102, West Bengal, India.
Ipsita K
IK
Sen
Department of Chemistry and Chemical Technology, Vidyasagar University, Midnapore 721102, West Bengal, India.
Ashis K
AK
Nandi
Department of Chemistry and Chemical Technology, Vidyasagar University, Midnapore 721102, West Bengal, India.
Bibhash C
BC
Panda
Immunology and Microbiology Laboratory, Department of Human Physiology with Community Health, Vidyasagar University, Midnapore 721102, West Bengal, India.
Sourav
S
Chattopadhyay
Immunology and Microbiology Laboratory, Department of Human Physiology with Community Health, Vidyasagar University, Midnapore 721102, West Bengal, India.
Somenath
S
Roy
Regional Plant Resource Centre, Bhubaneswar 751015, Odisha, India.
Atish K
AK
Sahoo
Regional Plant Resource Centre, Bhubaneswar 751015, Odisha, India.
Nibha
N
Gupta
Department of Chemistry and Chemical Technology, Vidyasagar University, Midnapore 721102, West Bengal, India. Electronic address: sirajul_1999@yahoo.com.
Syed S
SS
Islam
D000363:Agaricales / Q000737:chemistry* / Q000378:metabolism; D002240:Carbohydrate Sequence; D002470:Cell Survival / Q000187:drug effects; D048690:Fruiting Bodies, Fungal / Q000737:chemistry* / Q000378:metabolism; D005643:Fucose / Q000737:chemistry; D062610:Fungal Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D005690:Galactose / Q000737:chemistry; D005947:Glucose / Q000737:chemistry; D006027:Glycosides / Q000737:chemistry; D006358:Hot Temperature; D006801:Humans; D006868:Hydrolysis; D015227:Lipid Peroxidation / Q000187:drug effects; D059625:Liquid-Liquid Extraction / Q000379:methods; D008214:Lymphocytes / Q000166:cytology / Q000187:drug effects* / Q000201:enzymology; D008970:Molecular Weight; D010084:Oxidation-Reduction / Q000187:drug effects; D012995:Solubility; D014867:Water / Q000737:chemistry
D062610:Fungal Polysaccharides; D006027:Glycosides; D014867:Water; D005643:Fucose; D005947:Glucose; D005690:Galactose
Biological studies; NMR analysis; Tuber rufum (Pico) var (Heteropolysaccharide)