Inhibitory effect ofLactobacillus plantarumandLb. fermentumisolated from the faeces of healthy infants against nonfermentative bacteria causing nosocomial infections.
Nosocomial infection constitutes a major public health problem worldwide. Increasing antibiotic resistance of pathogens associated with nosocomial infections has also become a major therapeutic challenge for physicians. Thus, development of alternative treatment protocols, such as the use of probiotics, matters. The aim of this research was to determine the antagonistic properties ofLactobacillus plantarumandLb. fermentumisolated from the faeces of healthy infants against nonfermentative bacteria causing nosocomial infections. One hundred five samples of nosocomial infections were collected and processed for bacterial isolation and antimicrobial susceptibility testing following standard bacteriologic techniques. The antibiotic susceptibility test was performed by the disk diffusion method, and antagonistic effect ofLactobacillusstrains was investigated by well diffusion method. Of 105 samples, a total of 29 bacterial strains were identified as nonfermentative bacteria, including 17Acinetobacter baumanniiand 12Pseudomonas aeruginosa.A. baumanniishowed high resistance to tested antibiotics except ampicillin/sulbactam, andP. aeruginosashowed resistance to ampicillin/sulbactam and gentamicin and sensitive to amikacin and meropenem.Lb. plantarumhad antagonistic properties against bothA. baumanniiandP. aeruginosastrains. Lb. plantarumhad considerable effects on preventing the growth ofA. baumanniiandP. aeruginosastrains.However, further research is needed to better understanding of these effects onP. aeruginosa.
Food Microbiology Research Center, Tehran University of Medical Sciences, Iran; Division of Food Microbiology, Department of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Iran.
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Soltan Dallal
Department of Microbiology, Medical School, Babol University of Medical Science, Babol, Iran.
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Davoodabadi
Division of Bacteriology, Department of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Iran.
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Abdi
Department of Infectious and Tropical Diseases, Imam Khomeini Hospital, Tehran University of Medical Sciences, Iran.
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Hajiabdolbaghi
Zoonosis Research Center, Tehran University of Medical Sciences, Iran; Department of Medical Laboratory Sciences, School of Para Medicine, Tehran University of Medical Sciences, Tehran, Iran.
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Sharifi Yazdi
Food Microbiology Research Center, Tehran University of Medical Sciences, Iran; Division of Bacteriology, Department of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Iran.
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Douraghi
Food Microbiology Research Center, Tehran University of Medical Sciences, Iran.
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Tabatabaei Bafghi
Antimicrobial resistance; Lactobacillus; nonfermentative bacteria; nosocomial; probiotics