Noscapine targets EGFRp-Tyr1068to suppress the proliferation and invasion of MG63 cells.
Osteosarcoma, the most common primary malignant bone tumor, usually arises in the metaphysis of long bones. Amplification and mutation of the epidermal growth factor receptor (EGFR) gene represent signature genetic abnormalities encountered in osteosarcoma. Noscapine is a benzylisoquinoline alkaloid derived from the opium poppy Papaver somniferum. Recently several studies have suggested its anti-cancer effect in melanoma, ovarian cancer, gliomas, breast cancer, lung cancer, and colon cancer. However, the underlying molecular mechanism for its anti-cancer effect still remains unclear. In this paper, we found the mechanism of noscapine effectively suppressed proliferation and invasion of MG63 cell line by inhibiting the phosphorylation of EGFR and its downstream pathway.
Department of Orthopedic Surgery, Shengjing Hospital of China Medical University, Shenyang, Liaoning, People's Republic of China.
Ming
M
He
Department of Electrotheropy, Shenyang Medical College Affiliated Central Hospital, Shenyang, Liaoning, People's Republic of China.
Linlin
L
Jiang
Department of Orthopedic Surgery, Shengjing Hospital of China Medical University, Shenyang, Liaoning, People's Republic of China.
Zhaozhou
Z
Ren
Department of Orthopedic Surgery, Shengjing Hospital of China Medical University, Shenyang, Liaoning, People's Republic of China.
Guangbin
G
Wang
Department of Orthopedic Surgery, Shengjing Hospital of China Medical University, Shenyang, Liaoning, People's Republic of China.
Jiashi
J
Wang
D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D001859:Bone Neoplasms / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D066246:ErbB Receptors / Q000037:antagonists & inhibitors* / Q000378:metabolism; D006801:Humans; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D015394:Molecular Structure; D058990:Molecular Targeted Therapy; D009361:Neoplasm Invasiveness; D009363:Neoplasm Proteins / Q000037:antagonists & inhibitors* / Q000378:metabolism; D009665:Noscapine / Q000737:chemistry / Q000494:pharmacology*; D012516:Osteosarcoma / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D010207:Papaver / Q000737:chemistry; D010766:Phosphorylation / Q000187:drug effects; D019000:Phosphotyrosine / Q000032:analysis; D047428:Protein Kinase Inhibitors / Q000494:pharmacology*; D011499:Protein Processing, Post-Translational / Q000187:drug effects*; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics; D015398:Signal Transduction / Q000187:drug effects; D023041:Xenograft Model Antitumor Assays
D000972:Antineoplastic Agents, Phytogenic; D009363:Neoplasm Proteins; D047428:Protein Kinase Inhibitors; D034741:RNA, Small Interfering; D019000:Phosphotyrosine; D009665:Noscapine; C512478:EGFR protein, human; D066246:ErbB Receptors