An intracellular protein delivery platform based on glutathione-responsive protein nanocapsules.
An efficient strategy for the intracellular delivery of proteins was developed based on assembling proteins with a self-crosslinkable polymer. Such a disulfide-crosslinking structure enhances the stability of the protein-polymer assembly, and also allows effective dissociation of the assembly in response to glutathione, which allows effective delivery of various proteins with high intracellular bioactivity.
Department of Chemical and Biomolecular Engineering, University of California, Los Angeles, Los Angeles, CA 90095, USA. luucla@ucla.edu.
Jie
J
Li
Department of Nuclear Medicine, Xinhua Hospital Affiliated to Shanghai Jiao Tong University, Shanghai Jiao Tong University, Shanghai 200092, China. wanghuishanghai@hotmail.com.
Linlin
L
Zhang
Department of Chemical and Biomolecular Engineering, University of California, Los Angeles, Los Angeles, CA 90095, USA. luucla@ucla.edu and Key Laboratory of Functional Polymer Materials of Ministry of Education and State Key Laboratory of Medicinal Chemical Biology, Institute of Polymer Chemistry, Nankai University, Tianjin 300071, China.
Yang
Y
Liu
Department of Microbiology, Immunology, and Molecular Genetics, University of California, Los Angeles, CA 90095, USA.
Jing
J
Wen
Department of Chemical and Biomolecular Engineering, University of California, Los Angeles, Los Angeles, CA 90095, USA. luucla@ucla.edu.
Di
D
Wu
Department of Chemical and Biomolecular Engineering, University of California, Los Angeles, Los Angeles, CA 90095, USA. luucla@ucla.edu.
Duo
D
Xu
Department of Chemical and Biomolecular Engineering, University of California, Los Angeles, Los Angeles, CA 90095, USA. luucla@ucla.edu.
Tatiana
T
Segura
Diagnosis Biotech Co., Ltd, No. 3 Hengda Road, Qixia District, Nanjing, Jiangsu 210038, China. jjin.seu@gmail.com.
Jing
J
Jin
Department of Chemical and Biomolecular Engineering, University of California, Los Angeles, Los Angeles, CA 90095, USA. luucla@ucla.edu.
Yunfeng
Y
Lu
Department of Nuclear Medicine, Xinhua Hospital Affiliated to Shanghai Jiao Tong University, Shanghai Jiao Tong University, Shanghai 200092, China. wanghuishanghai@hotmail.com.
Hui
H
Wang
D002470:Cell Survival / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D005978:Glutathione / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D015394:Molecular Structure; D053769:Nanocapsules / Q000737:chemistry*; D010316:Particle Size; D011073:Polyamines / Q000737:chemistry* / Q000494:pharmacology; D011506:Proteins / Q000737:chemistry*; D013329:Structure-Activity Relationship
D053769:Nanocapsules; D011073:Polyamines; D011506:Proteins; C063994:polyallylamine; D005978:Glutathione