IL-37 impairs host resistance to Listeria infection by suppressing macrophage function.
Listeria monocytogenes is a Gram-positive intracellular bacterium that was transmitted through contaminated food and causes sepsis and even death. IL-37 has been described as an important anti-inflammatory factor, but little is known about the function of IL-37 in host defense against Liseria monocytogenes (Lm) infection. In mice model of systemic infection, we found that mice treated with IL-37 were more sensitive to Lm infection compared with PBS-treated mice. This reduced resistance to Lm in IL-37-treated mice is accompanied with increased bacterial burden and liver damage. Serum levels of colony-stimulating factors were decreased in IL-37-treated mice. IL-37 treatment reduced bactericidal ability of bone marrow derived macrophages (BMDMs) in vitro, which contribute to the inability of IL-37-treated mice to combat Lm infection. Furthermore, increased apoptosis was observed in Lm-infected macrophages treated with IL-37. Increased macrophage apoptosis reduced percentage in liver macrophages was observed in IL-37-treated mice following Lm infection. These results indicate the negative regulatory effect of IL-37 on host resistance during immune defense against Lm.
Shanghai Tenth People's Hospital, Tongji University School of Medicine, Shanghai, 200092, China.
Mengmeng
M
Zhao
Shanghai Tenth People's Hospital, Tongji University School of Medicine, Shanghai, 200092, China.
Yongguang
Y
Hu
Shanghai Tenth People's Hospital, Tongji University School of Medicine, Shanghai, 200092, China.
Juanjuan
J
Shou
Shanghai Tenth People's Hospital, Tongji University School of Medicine, Shanghai, 200092, China; State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou, China.
Shao Bo
SB
Su
Shanghai Tenth People's Hospital, Tongji University School of Medicine, Shanghai, 200092, China.
Jianhua
J
Yang
Shanghai Tenth People's Hospital, Tongji University School of Medicine, Shanghai, 200092, China. Electronic address: tianshuy@tongji.edu.cn.
Tianshu
T
Yang
D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D058491:Bacterial Load / Q000187:drug effects; D002478:Cells, Cultured; D016207:Cytokines / Q000097:blood; D004195:Disease Models, Animal; D060467:Disease Resistance / Q000187:drug effects* / Q000276:immunology; D005434:Flow Cytometry; D054884:Host-Pathogen Interactions / Q000187:drug effects; D006801:Humans; D007375:Interleukin-1 / Q000494:pharmacology*; D008089:Listeria monocytogenes / Q000187:drug effects* / Q000276:immunology / Q000502:physiology; D008088:Listeriosis / Q000097:blood / Q000188:drug therapy* / Q000382:microbiology; D008099:Liver / Q000187:drug effects / Q000382:microbiology / Q000473:pathology; D008264:Macrophages / Q000187:drug effects* / Q000276:immunology / Q000382:microbiology; D008807:Mice, Inbred BALB C; D016019:Survival Analysis
D016207:Cytokines; C508587:IL37 protein, human; D007375:Interleukin-1
Apoptosis; Interleukin-37; Liseria monocytogenes; Macrophage