MicroRNA Expression Profiling Using Agilent One-Color Microarray.
MicroRNA (miRNA) expression profiling is an important tool to identify miRNA regulation in physiological or pathological states. This technique has a large number of molecular diagnostic applications, including in cancer, cardiovascular and autoimmune diseases, and forensics. To date, a multitude of high-throughput genomic approaches have been developed. Here, we focus on miRNA expression profiling by microarray using SurePrint technology, providing a description of both the workflow and methods for expression profiling by Agilent One-Color Microarray.
Institute of Genetics and Biophysics, CNR, 80131, Naples, Italy. dellaversana@igb.cnr.it.|Department of Biochemistry, Biophysics and General Pathology, Second University of Naples, Via Antonio Vivaldi, 43, 80138, Naples, Italy. dellaversana@igb.cnr.it.
Carmela
C
Dell'Aversana
Department of Biochemistry, Biophysics and General Pathology, Second University of Naples, Via Antonio Vivaldi, 43, 80138, Naples, Italy.
Cristina
C
Giorgio
Institute of Genetics and Biophysics, CNR, 80131, Naples, Italy.|Department of Biochemistry, Biophysics and General Pathology, Second University of Naples, Via Antonio Vivaldi, 43, 80138, Naples, Italy.
Lucia
L
Altucci
D000818:Animals; D020869:Gene Expression Profiling / Q000379:methods*; D006801:Humans; D035683:MicroRNAs / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis
D035683:MicroRNAs
Expression profile; Microarray; Platform; Slide; miRNA