A glyphosate-based herbicide induces histomorphological and protein expression changes in the liver of the female guppy Poecilia reticulata.
Glyphosate-based herbicides (GBH) are among the most common herbicides found in aquatic systems, but limited data are available about their mode of action and hepatotoxicity in fish. This study investigated the hepatotoxicity induced by GBH in the guppy Poecilia reticulata using a histopathological assessment associated with a proteomic approach. Guppies were exposed to GBH for 24 h at 1.8 mg of glyphosate L-1, corresponding to 50% of the LC50, 96 h. The results indicate that the GBH at 1.8 mg of glyphosate L-1induce the development of hepatic damage in P. reticulata, which is exposure-time dependent. The histopathological indexes demonstrate that GBH cause inflammatory, regressive, vascular and progressive disorders in the liver of guppies. Using 2D gel electrophoresis associated with mass spectrometry, 18 proteins that changed by GBH were identified and were related to the cellular structure, motility and transport, energy metabolism and apoptosis. The results show that the acute exposure to GBH causes hepatic histopathological damage related to protein expression profile changes in P. reticulata, indicating that a histopathological assessment associated with a proteomic analysis provides a valuable approach to assess the toxic effects of GBH in sentinel fish species.
Laboratory of Cellular Behavior, Biological Sciences Institute, Federal University of Goiás, Goiânia, Goiás, Brazil.
Ana Paula Rezende
APR
Dos Santos
Laboratory of Cellular Behavior, Biological Sciences Institute, Federal University of Goiás, Goiânia, Goiás, Brazil; CIMA, Faculty of Science and Technology, University of Algarve, Campus de Gambelas, Faro, Portugal.
Thiago Lopes
TL
Rocha
Laboratory of Molecular Biology, Biological Sciences Institute, Federal University of Goiás, Goiânia, Goiás, Brazil.
Clayton Luiz
CL
Borges
Laboratory of Molecular Biology, Biological Sciences Institute, Federal University of Goiás, Goiânia, Goiás, Brazil.
Alexandre Melo
AM
Bailão
Laboratory of Molecular Biology, Biological Sciences Institute, Federal University of Goiás, Goiânia, Goiás, Brazil.
Célia Maria
CM
de Almeida Soares
Laboratory of Cellular Behavior, Biological Sciences Institute, Federal University of Goiás, Goiânia, Goiás, Brazil. Electronic address: simonesaboias@gmail.com.
Simone Maria Teixeira
SMT
de Sabóia-Morais
D000818:Animals; D056486:Chemical and Drug Induced Liver Injury / Q000378:metabolism / Q000473:pathology; D005260:Female; D005998:Glycine / Q000031:analogs & derivatives* / Q000633:toxicity; D006540:Herbicides / Q000633:toxicity*; D008099:Liver / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D008107:Liver Diseases; D011036:Poecilia; D040901:Proteomics; D000097797:Glyphosate
D005998:Glycine; D006540:Herbicides; D000097797:Glyphosate
Biomarkers; Ecotoxicology; Histopathology; Proteomics; Roundup