Hyaluronic Acid/PLGA Core/Shell Fiber Matrices Loaded with EGCG Beneficial to Diabetic Wound Healing.
During the last few decades, considerable research on diabetic wound healing strategies has been performed, but complete diabetic wound healing remains an unsolved problem, which constitutes an enormous biomedical burden. Herein, hyaluronic acid (HA)/poly(lactic-co-glycolic acid, PLGA) core/shell fiber matrices loaded with epigallocatechin-3-O-gallate (EGCG) (HA/PLGA-E) are fabricated by coaxial electrospinning. HA/PLGA-E core/shell fiber matrices are composed of randomly-oriented sub-micrometer fibers and have a 3D porous network structure. EGCG is uniformly dispersed in the shell and sustainedly released from the matrices in a stepwise manner by controlled diffusion and PLGA degradation over four weeks. EGCG does not adversely affect the thermomechanical properties of HA/PLGA-E matrices. The number of human dermal fibroblasts attached on HA/PLGA-E matrices is appreciably higher than that on HA/PLGA counterparts, while their proliferation is steadily retained on HA/PLGA-E matrices. The wound healing activity of HA/PLGA-E matrices is evaluated in streptozotocin-induced diabetic rats. After two weeks of surgical treatment, the wound areas are significantly reduced by the coverage with HA/PLGA-E matrices resulting from enhanced re-epithelialization/neovascularization and increased collagen deposition, compared with no treatment or HA/PLGA. In conclusion, the HA/PLGA-E matrices can be potentially exploited to craft strategies for the acceleration of diabetic wound healing and skin regeneration.
Department of Cogno-Mechatronics Engineering, College of Nanoscience & Nanotechnology, Pusan National University, Busan, 46241, Korea.
Yong Cheol
YC
Shin
Research Center for Energy Convergence Technology, Pusan National University, Busan, 46241, Korea.
Dong-Myeong
DM
Shin
Department of Cogno-Mechatronics Engineering, College of Nanoscience & Nanotechnology, Pusan National University, Busan, 46241, Korea.
Eun Ji
EJ
Lee
Department of Cogno-Mechatronics Engineering, College of Nanoscience & Nanotechnology, Pusan National University, Busan, 46241, Korea.
Jong Ho
JH
Lee
Department of Biomaterials Science, College of Natural Resources and Life Science, Life and Industry Convergence Research Institute, Pusan National University, Miryang, 50463, Korea.
Ji Eun
JE
Kim
Department of Biomaterials Science, College of Natural Resources and Life Science, Life and Industry Convergence Research Institute, Pusan National University, Miryang, 50463, Korea.
Sung Hwa
SH
Song
Department of Biomaterials Science, College of Natural Resources and Life Science, Life and Industry Convergence Research Institute, Pusan National University, Miryang, 50463, Korea.
Dae-Youn
DY
Hwang
Department of Prosthodontics, Dental Research Institute, School of Dentistry, Seoul National University, Seoul, 03080, Korea.
Jun Jae
JJ
Lee
Dental Life Science Research Institute, Seoul National University Dental Hospital, Seoul, 03080, Korea.
Bongju
B
Kim
Department of Mechanical Engineering, Sejong University, Seoul, 05006, Korea.
Dohyung
D
Lim
Center for Fiber and Textile Science, Kyoto Institute of Technology, Kyoto, 606-8585, Japan.
Suong-Hyu
SH
Hyon
Department of Prosthodontics, Dental Research Institute, School of Dentistry, Seoul National University, Seoul, 03080, Korea.
Young-Jun
YJ
Lim
Department of Cogno-Mechatronics Engineering, College of Nanoscience & Nanotechnology, Pusan National University, Busan, 46241, Korea.
Dong-Wook
DW
Han
D000818:Animals; D002392:Catechin / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000737:chemistry; D003094:Collagen / Q000378:metabolism; D003921:Diabetes Mellitus, Experimental / Q000188:drug therapy*; D005347:Fibroblasts / Q000187:drug effects; D006820:Hyaluronic Acid / Q000008:administration & dosage* / Q000737:chemistry*; D018919:Neovascularization, Physiologic / Q000187:drug effects; D011100:Polyglycolic Acid / Q000008:administration & dosage / Q000737:chemistry*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012867:Skin / Q000187:drug effects; D014945:Wound Healing / Q000187:drug effects*
D011100:Polyglycolic Acid; D002392:Catechin; D006820:Hyaluronic Acid; D003094:Collagen; C045651:epigallocatechin gallate
core/shell fiber matrix; diabetic wound healing; epigallocatechin-3-O-gallate; hyaluronic acid; poly(lactic-co-glycolic acid)