Analysis of the Distribution Profiles of Circulating MicroRNAs by Asymmetrical Flow Field Flow Fractionation.
MicroRNAs (miRNAs) are stably present in circulatory systems. They are bound to various carriers like proteins, lipoprotein particles, and exosomes. Investigating the process of miRNA distribution among these carriers will help improve our understanding of their functions in the extracellular environment and their potential relationship with diseases. Here, we describe how to obtain the distribution profiles of circulating miRNAs by separation of different miRNA carriers in human serum with asymmetrical flow field flow fractionation (AF4), and detection of the miRNAs in the eluted fractions that enrich particular types of carriers with RT-qPCR.
Department of Chemistry, University of California, Riverside, CA, USA.
Kenneth
K
Flack
Program in Biomedical Sciences, University of California, Riverside, CA, USA.
Luis A
LA
Jimenez
Department of Chemistry, University of California, Riverside, CA, USA. wenwan.zhong@ucr.edu.
Wenwan
W
Zhong
D014408:Biomarkers, Tumor / Q000097:blood / Q000235:genetics / Q000302:isolation & purification*; D002853:Chromatography, Liquid / Q000379:methods; D000074166:Circulating MicroRNA / Q000097:blood / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D020133:Reverse Transcriptase Polymerase Chain Reaction
D014408:Biomarkers, Tumor; D000074166:Circulating MicroRNA
Cancer biomarkers; Circulating microRNA; Exosomes; Extracellular miRNA carriers; Fractionation; Lipoprotein