Microbiological and clinical assessment of the abutment and non-abutment teeth of partial removable denture wearers.
OBJECTIVE
The aim of this study was assessing the changes in both clinical and microbiological parameters of healthy individuals after rehabilitation with removable partial denture (RPD).


METHODS
11 women received unilateral or bilateral free-end saddle RPD in the mandibular arch. Clinical and microbiological parameters of abutment, non-abutment, and antagonist teeth were assessed at baseline (RPD installation) and after 7, 30, 90, and 180days of function. The Checkerboard DNA-DNA hybridization technique was used to identify and quantify up to 43 different microbial species from subgingival biofilm samples. Probing depth, gingival recession, and bleeding on probing were also investigated over time.


RESULTS
The total and individual microbial genome counts were shown significantly increased after 180days with no significant differences between abutment, non-abutment, or antagonist teeth. Streptococcus spp., Aggregatibacter actinomycetemcomitans, and other species associated to periodontitis (Peptostreptococcus anaerobius, Prevotella nigrescens, and Tannerella forsythia), as well as opportunistic Candida spp., were recovered in moderate counts. Abutment teeth presented higher values of gingival recession when compared with non-abutment or antagonist teeth, irrespectively time of sampling (p<0.05). No significant differences were found between groups regarding bleeding on probing or probing depth over time.


CONCLUSIONS
Overall, the microbial counts significantly increased after 6 months of denture loading for both abutment and non-abutment teeth with no significant differences regarding the microbial profile over time. Bleeding on probing and probing depth showed no significant difference between groups over time whereas gingival recession increased in the abutment teeth.
Department of Dental Materials and Prosthesis, School of Dentistry of Ribeirão Preto, University of São Paulo, Av. do Café s/n°, Monte Alegre, Ribeirão Preto-SP, 14040-904, Brazil.
Luciana
L
Costa
Department of Dental Materials and Prosthesis, School of Dentistry of Ribeirão Preto, University of São Paulo, Av. do Café s/n°, Monte Alegre, Ribeirão Preto-SP, 14040-904, Brazil.
Cássio
C
do Nascimento
Department of Dental Materials and Prosthesis, School of Dentistry of Ribeirão Preto, University of São Paulo, Av. do Café s/n°, Monte Alegre, Ribeirão Preto-SP, 14040-904, Brazil.
Valéria Oliveira Pagnano
VO
de Souza
Department of Dental Materials and Prosthesis, School of Dentistry of Ribeirão Preto, University of São Paulo, Av. do Café s/n°, Monte Alegre, Ribeirão Preto-SP, 14040-904, Brazil. Electronic address: pedrazzi@forp.usp.br.
Vinícius
V
Pedrazzi
D000328:Adult; D000368:Aged; D001419:Bacteria / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D058491:Bacterial Load; D018441:Biofilms; D002175:Candida / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D015342:DNA Probes; D000044:Dental Abutments / Q000382:microbiology*; D003832:Denture, Partial, Removable / Q000382:microbiology*; D005260:Female; D064349:Genome, Microbial; D005889:Gingival Recession; D006801:Humans; D008334:Mandible; D008875:Middle Aged; D010512:Periodontal Index; D010514:Periodontal Pocket; D013997:Time Factors
D015342:DNA Probes
Clinical assessment; DNA probes; Microbiology; Partial removable denture