Occurrence and antimicrobial susceptibility of enteric rods and pseudomonads isolated from the dental prostheses biofilm.
To determine the occurrence and the in vitro antimicrobial susceptibility of enteric rods and pseudomonads from the denture biofilm of 52 subjects at the Center for Dental Specialties of Sobral/ Ceara, Brazil.
Denture biofilm was collected and samples plated on MacConkey agar. The isolated bacterial colonies were identified using the BBL Crystal enteric/non-fermenter system. Antibiotic bacterial susceptibility was assessed by the disc diffusion method of amoxicillin, amoxicillin/clavulanic acid, doxycycline, tetracycline, tobramycin, imipenem, cefotaxime, and ciprofloxacin. The Minimum Inhibitory Concentration (MIC) of cefotaxime, tobramycin, doxycycline, imipenem, and ciprofloxacin was determined for 40 species by E-test.
34 subjects (65.4%) harbored enteric rods in their prostheses. Klebsiella pneumoniae (26.5%), Escherichia coli (23.5%), and Enterobacter aerogenes (23.5%) were the most prevalent species. All organisms were susceptible to ciprofloxacin and most species were resistant to amoxicillin or amoxicillin/clavulanic acid, demonstrating variable sensitivity patterns to other antimicrobials. However, the MIC showed the emergence of strains with reduced sensitivity to ciprofloxacin (MIC90≥3 μg/ mL) and cefotaxime (MIC90≥2 μg/mL).
The findings show high prevalence of nosocomial diseases-related bacterial species and low susceptibility to antimicrobial drugs. Therefore, these results imply caution against the indiscriminate use of broad spectrum antibiotics in dental practice.
Universidade Federal do Ceará, Faculdade de Odontologia, Campus Sobral, Sobral, CE, Brasil.
Sanrrangers Sales
SS
Silva
Universidade Federal do Ceará, Faculdade de Medicina, Campus Sobral,Sobral, CE, Brasil.
Maximilo de Oliveira
MO
Ribeiro
Universidade Federal do Ceará, Faculdade de Odontologia, Campus Sobral, Sobral, CE, Brasil.
Francisco Isaac Fernandes
FI
Gomes
Universidade Federal do Ceará, Faculdade de Odontologia, Campus Sobral, Sobral, CE, Brasil.
Hellíada Vasconcelos
HV
Chaves
Agência Nacional de Vigilância Sanitária, Brasília, DF, Brasil.
Antonio Alfredo Rodrigues E
AA
Silva
Universidade Federal do Ceará, Faculdade de Odontologia, Campus Sobral, Sobral, CE, Brasil.
Iriana Carla Junqueira
IC
Zanin
Universidade Federal do Ceará, Faculdade de Odontologia, Campus Sobral, Sobral, CE, Brasil.
Francisco Cesar Barroso
FC
Barbosa
D000328:Adult; D017677:Age Distribution; D000368:Aged; D000369:Aged, 80 and over; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D018441:Biofilms / Q000254:growth & development*; D017266:Dental Prosthesis / Q000382:microbiology*; D024901:Drug Resistance, Multiple, Bacterial; D004755:Enterobacteriaceae / Q000187:drug effects / Q000302:isolation & purification*; D005260:Female; D006801:Humans; D008297:Male; D008826:Microbial Sensitivity Tests; D008875:Middle Aged; D011549:Pseudomonas / Q000187:drug effects / Q000302:isolation & purification*; D012016:Reference Values; D017678:Sex Distribution; D018709:Statistics, Nonparametric; D013997:Time Factors
D000900:Anti-Bacterial Agents