Tonsil-Derived Mesenchymal Stem Cells Differentiate into a Schwann Cell Phenotype and Promote Peripheral Nerve Regeneration.
Schwann cells (SCs), which produce neurotropic factors and adhesive molecules, have been reported previously to contribute to structural support and guidance during axonal regeneration; therefore, they are potentially a crucial target in the restoration of injured nervous tissues. Autologous SC transplantation has been performed and has shown promising clinical results for treating nerve injuries and donor site morbidity, and insufficient production of the cells have been considered as a major issue. Here, we performed differentiation of tonsil-derived mesenchymal stem cells (T-MSCs) into SC-like cells (T-MSC-SCs), to evaluate T-MSC-SCs as an alternative to SCs. Using SC markers such asCAD19,GFAP,MBP,NGFR,S100B, andKROX20during quantitative real-time PCR we detected the upregulation ofNGFR,S100B, andKROX20and the downregulation ofCAD19andMBPat the fully differentiated stage. Furthermore, we found myelination of axons when differentiated SCs were cocultured with mouse dorsal root ganglion neurons. The application of T-MSC-SCs to a mouse model of sciatic nerve injury produced marked improvements in gait and promoted regeneration of damaged nerves. Thus, the transplantation of human T-MSCs might be suitable for assisting in peripheral nerve regeneration.
Department of Biochemistry, School of Medicine, Ewha Womans University, Seoul 07985, Korea. polypola@ewhain.net.
Namhee
N
Jung
Department of Biochemistry, School of Medicine, Ewha Womans University, Seoul 07985, Korea. saeyoung@ewha.ac.kr.
Saeyoung
S
Park
Department of Biochemistry, School of Medicine, Ewha Womans University, Seoul 07985, Korea. yychoi27@ewhain.net.
Yoonyoung
Y
Choi
Department of Biochemistry, School of Medicine, Ewha Womans University, Seoul 07985, Korea. joowon.park@ewha.ac.kr.
Joo-Won
JW
Park
Stem Cell & Regenerative Medicine Institute, Samsung Medical Center, Seoul 06351, Korea. ybhong@skku.edu.
Young Bin
YB
Hong
Department of Neuroscience and Behavioral Biology, Emory University, Atlanta, GA 30322, USA. hcpark2@emory.edu.
Hyun Ho Choi
HH
Park
Department of Molecular Medicine, School of Medicine, Ewha Womans University, Seoul 07985, Korea. rys75@ewha.ac.kr.
Yeonsil
Y
Yu
Department of Health Sciences and Technology, SAIHST, Sungkyunkwan University, Seoul 06351, Korea. kgun903@naver.com.
Geon
G
Kwak
Department of Otorhinolaryngology-Head & Neck Surgery, School of Medicine, Ewha Womans University, Seoul 07985, Korea. sevent@ewha.ac.kr.
Han Su
HS
Kim
Department of Pediatrics, School of Medicine, Ewha Womans University, Seoul 07985, Korea. ykh@ewha.ac.kr.
Kyung-Ha
KH
Ryu
Department of Orthopedic Surgery, School of Medicine, Ewha Womans University, Seoul 07985, Korea. kimjk@ewha.ac.kr.
Jae Kwang
JK
Kim
Department of Molecular Medicine, School of Medicine, Ewha Womans University, Seoul 07985, Korea. inhojo@ewha.ac.kr.
Inho
I
Jo
Department of Neurology, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul 06351, Korea. bochoi77@hanmail.net.
Byung-Ok
BO
Choi
Department of Biochemistry, School of Medicine, Ewha Womans University, Seoul 07985, Korea. jungsc@ewha.ac.kr.
Sung-Chul
SC
Jung
D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D002454:Cell Differentiation; D002648:Child; D018920:Coculture Techniques; D005727:Ganglia, Spinal / Q000166:cytology / Q000378:metabolism; D015870:Gene Expression; D006801:Humans; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D009416:Nerve Regeneration / Q000502:physiology*; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism; D009474:Neurons / Q000166:cytology / Q000378:metabolism; D014066:Palatine Tonsil / Q000166:cytology / Q000378:metabolism / Q000601:surgery; D059348:Peripheral Nerve Injuries / Q000378:metabolism / Q000473:pathology / Q000534:rehabilitation* / Q000601:surgery; D020127:Recovery of Function; D012583:Schwann Cells / Q000166:cytology* / Q000378:metabolism / Q000637:transplantation; D012584:Sciatic Nerve / Q000293:injuries* / Q000378:metabolism; D014068:Tonsillectomy; D014183:Transplantation, Heterologous
D015415:Biomarkers; D009419:Nerve Tissue Proteins
Schwann cell; differentiation; peripheral nerve; regeneration; tonsil-derived mesenchymal stem cells