Model acupuncture point: Bone marrow-derived stromal stem cells are moved by a weak electromagnetic field.
OBJECTIVE
To show the existence of a structural formative role of magnetic fields (MFs) with respect to biological objects by using our proposed model of an acupoint.


METHODS
We introduced a magnetised 10-100 μT metal rod (needle) into culture dishes with a negatively charged working surface and observed during 24 h how cells were arranged by MFs and by electrical fields (EFs) when attached. Rat and human bone marrow-derived stromal stem cells (rBMSCs and hBMSCs), human nonadherent mononuclear blood cells, NCTCs and A172 cells, andEscherichia coli(E. coli) were evaluated. The dish containing BMSCs was defined as the model of an acupoint. rBMSCs proliferative activity affected by the needle was investigated. For investigating electromagnetic field structures, we used the gas discharge visualisation (GDV) method.


RESULTS
During 24 h of incubation in 50-mm culture dishes, BMSCs or the nonadherent cells accumulated into a central heap in each dish. BMSCs formed a torus (central ring) with an inner diameter of approximately 10 mm only upon the introduction of the needle in the centre of the dish. The cells did not show these effects in 35- or 90-mm culture dishes or hydrophobic dishes or rectangular cuvettes. NCTCs and A172 cells showed unstable the effects and only up to two weeks after thawing. Moreover, we observed that the appearance of these effects depended on the season. In winter, BMSCs showed no the effects. GDV experiments revealed that the resonant annular illumination gradually formed from 10 to 18-20 s in polar solutions with and without cell suspension of BMSCs, NCTCs andE. coliwhen using circular 50-mm dishes, stimulation at 115 V and switching of the electrode poles at 1 kHz. All these data demonstrate the resonant nature of the central ring. Significant influence of MFs on the rBMSC proliferation rate was not observed.


CONCLUSIONS
BMSCs can be moved by MFs when in the presence of a constant EF and MF, when the cells are in the responsive functional state, and when there is a resonant relationship between them.
Artem N Emelyanov, Laboratory of High Laser and Magnetic Technology, North-Western State Medical University, 191015 St. Petersburg, Russia.
Artem N
AN
Emelyanov
Artem N Emelyanov, Laboratory of High Laser and Magnetic Technology, North-Western State Medical University, 191015 St. Petersburg, Russia.
Marina V
MV
Borisova
Artem N Emelyanov, Laboratory of High Laser and Magnetic Technology, North-Western State Medical University, 191015 St. Petersburg, Russia.
Vera V
VV
Kiryanova
Acupuncture; Biology resonance; Cytoplasm movement; Frizzled-related protein; Glycocalyx; Magnetic targeting; Model acupoint; Stem cell movement