Constitutive cellulase production from glucose using the recombinant Trichoderma reesei strain overexpressing an artificial transcription activator.
The high cost of cellulase production presents biggest challenge in biomass deconstruction. Cellulase production by Trichoderma reesei using low cost carbon source is of great interest. In this study, an artificial transcription activator containing the Cre1 binding domain linked to the Xyr1 effector and binding domains was designed and constitutively overexpressed in T. reesei RUT C30. The recombinant strain T. reesei zxy-2 displayed constitutive cellulase production using glucose as a sole carbon source, and the production titer was 12.75-fold of that observed with T. reesei RUT C30 in shake flask culture. Moreover, FPase and xylanase titers of 2.63 and 108.72IU/mL, respectively, were achieved using glucose as sole carbon source within 48h in a 7-L fermenter by batch fermentation using T. reesei zxy-2. The crude enzyme obtained was used to hydrolyze alkali pretreated corn stover, and a high glucose yield of 99.18% was achieved.
School of Life Science and Biotechnology, Dalian University of Technology, Dalian 116023, China.
Xiaoyue
X
Zhang
School of Life Science and Biotechnology, Dalian University of Technology, Dalian 116023, China.
Yonghao
Y
Li
State Key Laboratory of Microbial Metabolism & School of Life Science and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China. Electronic address: xqzhao@sjtu.edu.cn.
Xinqing
X
Zhao
School of Life Science and Biotechnology, Dalian University of Technology, Dalian 116023, China; State Key Laboratory of Microbial Metabolism & School of Life Science and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China.
Fengwu
F
Bai
D019149:Bioreactors / Q000382:microbiology; D002244:Carbon / Q000378:metabolism; D002480:Cellulase / Q000235:genetics* / Q000378:metabolism*; D005285:Fermentation / Q000235:genetics; D005656:Fungal Proteins / Q000378:metabolism; D005947:Glucose / Q000378:metabolism*; D006868:Hydrolysis; D030781:Organisms, Genetically Modified; D014157:Transcription Factors / Q000235:genetics*; D015533:Transcriptional Activation / Q000235:genetics; D019076:Transgenes; D014242:Trichoderma / Q000201:enzymology / Q000235:genetics* / Q000378:metabolism; D003313:Zea mays / Q000378:metabolism
D005656:Fungal Proteins; D014157:Transcription Factors; D002244:Carbon; D002480:Cellulase; D005947:Glucose
Artificial transcription factor; Cellulase production; Glucose; Trichoderma reesei; xyr1 and cre1