Genotyping DNA Variants with High-Resolution Melting Analysis.
High-resolution melting analysis (HRMA) is a simple, quick, and effective method to scan and screen PCR amplicons for sequence variants. HRMA is a nondestructive closed tube assay; after PCR, DNA melting can directly be performed on the amplified samples without any purification or separation steps. For single SNP genotyping, HRMA is an attractive alternative to Sanger sequencing, restriction enzyme analysis, and hydrolysis probes.
Department of Human Genetics, Leiden Genome Technology Center, Leiden University Medical Center, Einthovenweg 20, 2333 ZC, Leiden, The Netherlands. r.vossen@lumc.nl.
Rolf H A M
RH
Vossen
D000483:Alleles; D004247:DNA / Q000235:genetics*; D017931:DNA Primers; D005838:Genotype; D006801:Humans; D009691:Nucleic Acid Denaturation; D016133:Polymerase Chain Reaction / Q000379:methods; D020641:Polymorphism, Single Nucleotide
D017931:DNA Primers; D004247:DNA
DNA; Melting curve analysis; Single nucleotide polymorphism; Variant detection