Reconstruction of Alar Nasal Cartilage Defects Using a Tissue Engineering Technique Based on a Combined Use of Autologous Chondrocyte Micrografts and Platelet-rich Plasma: Preliminary Clinical and Instrumental Evaluation.
BACKGROUND
Developing cartilage constructs with injectability, appropriate matrix composition, and persistent cartilaginous phenotype remains an enduring challenge in cartilage repair. The combined use of autologous chondrocyte micrografts and platelet-rich plasma (PRP) is an alternative that opens a new era in this field.


METHODS
At the Department of Plastic and Reconstructive Surgery, University of Rome Tor Vergata, Italy, 11 patients underwent nasal alar reconstruction with chondrocyte micrografts gently poured onto PRP in solid form. A computed tomographic scan control was performed after 12 months. Pearson's Chi-square test was used to investigate difference in cartilage density between native and newly formed cartilages.


RESULTS
The constructs of chondrocyte micrografts-PRP that were subcutaneously injected resulted in a persistent cartilage tissue with appropriate morphology, adequate central nutritional perfusion without central necrosis or ossification, and further augmented nasal dorsum without obvious contraction and deformation.


CONCLUSIONS
This report demonstrated that chondrocyte micrografts derived from nasal septum poured onto PRP in solid form are useful for cartilage regeneration in patients with external nasal valve collapse.
Plastic and Reconstructive Surgery Department, University of Rome Tor Vergata, Rome, Italy; Plastic and Reconstructive Surgery, Catholic University, Tirana, Albania; and Institute of Anatomic Pathology, University of Rome Tor Vergata, Rome, Italy.
Pietro
P
Gentile
Plastic and Reconstructive Surgery Department, University of Rome Tor Vergata, Rome, Italy; Plastic and Reconstructive Surgery, Catholic University, Tirana, Albania; and Institute of Anatomic Pathology, University of Rome Tor Vergata, Rome, Italy.
Maria G
MG
Scioli
Plastic and Reconstructive Surgery Department, University of Rome Tor Vergata, Rome, Italy; Plastic and Reconstructive Surgery, Catholic University, Tirana, Albania; and Institute of Anatomic Pathology, University of Rome Tor Vergata, Rome, Italy.
Alessandra
A
Bielli
Plastic and Reconstructive Surgery Department, University of Rome Tor Vergata, Rome, Italy; Plastic and Reconstructive Surgery, Catholic University, Tirana, Albania; and Institute of Anatomic Pathology, University of Rome Tor Vergata, Rome, Italy.
Augusto
A
Orlandi
Plastic and Reconstructive Surgery Department, University of Rome Tor Vergata, Rome, Italy; Plastic and Reconstructive Surgery, Catholic University, Tirana, Albania; and Institute of Anatomic Pathology, University of Rome Tor Vergata, Rome, Italy.
Valerio
V
Cervelli