Microencapsulation of Hepatocytes and Mesenchymal Stem Cells for Therapeutic Applications.
Encapsulated hepatocyte transplantation and encapsulated mesenchymal stem cell transplantation are newly developed potential treatments for acute and chronic liver diseases, respectively. Cells are microencapsulated in biocompatible semipermeable alginate-based hydrogels. Microspheres protect cells against antibodies and immune cells, while allowing nutrients, small/medium size proteins and drugs to diffuse inside and outside the polymer matrix. Microencapsulated cells are assessed in vitro and designed for experimental transplantation and for future clinical applications.Here, we describe the protocol for microencapsulation of hepatocytes and mesenchymal stem cells within hybrid poly(ethylene glycol)-alginate hydrogels.
Department of Surgery, Visceral and Transplant Surgery, Geneva University Hospital and Medical School, Rue Gabrielle-Perret-Gentil 4, 1211, Geneva, Switzerland. Raphael.Meier@hcuge.ch.
Raphael P H
RP
Meier
Department of Surgery, Visceral and Transplant Surgery, Geneva University Hospital and Medical School, Rue Gabrielle-Perret-Gentil 4, 1211, Geneva, Switzerland.
Elisa
E
Montanari
Department of Surgery, Visceral and Transplant Surgery, Geneva University Hospital and Medical School, Rue Gabrielle-Perret-Gentil 4, 1211, Geneva, Switzerland.
Philippe
P
Morel
Department of Surgery, Visceral and Transplant Surgery, Geneva University Hospital and Medical School, Rue Gabrielle-Perret-Gentil 4, 1211, Geneva, Switzerland.
Joël
J
Pimenta
Department of Surgery, Visceral and Transplant Surgery, Geneva University Hospital and Medical School, Rue Gabrielle-Perret-Gentil 4, 1211, Geneva, Switzerland.
Henk-Jan
HJ
Schuurman
Interfaculty Institute of Bioengineering and Institute of Chemical Sciences and Engineering, Ecole Polytechnique Fédérale de Lausanne (EPFL), 1015, Lausanne, Switzerland.
Christine
C
Wandrey
Interfaculty Institute of Bioengineering and Institute of Chemical Sciences and Engineering, Ecole Polytechnique Fédérale de Lausanne (EPFL), 1015, Lausanne, Switzerland.
Sandrine
S
Gerber-Lemaire
Interfaculty Institute of Bioengineering and Institute of Chemical Sciences and Engineering, Ecole Polytechnique Fédérale de Lausanne (EPFL), 1015, Lausanne, Switzerland.
Redouan
R
Mahou
Department of Surgery, Visceral and Transplant Surgery, Geneva University Hospital and Medical School, Rue Gabrielle-Perret-Gentil 4, 1211, Geneva, Switzerland.
Leo H
LH
Bühler
D000464:Alginates / Q000737:chemistry; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry; D002214:Capsules / Q000737:chemistry*; D002454:Cell Differentiation; D049109:Cell Proliferation; D002470:Cell Survival; D002478:Cells, Cultured; D004195:Disease Models, Animal; D004339:Drug Compounding / Q000295:instrumentation / Q000379:methods*; D058625:End Stage Liver Disease / Q000628:therapy*; D020723:Glucuronic Acid / Q000737:chemistry; D022781:Hepatocytes / Q000502:physiology / Q000637:transplantation*; D006603:Hexuronic Acids / Q000737:chemistry; D006801:Humans; D020100:Hydrogels / Q000737:chemistry; D007120:Immunochemistry; D008099:Liver / Q000166:cytology / Q000473:pathology; D045164:Mesenchymal Stem Cell Transplantation / Q000295:instrumentation / Q000379:methods* / Q000401:mortality; D059630:Mesenchymal Stem Cells / Q000502:physiology; D051379:Mice; D011092:Polyethylene Glycols / Q000737:chemistry; D061251:Primary Cell Culture / Q000379:methods; D016019:Survival Analysis
D000464:Alginates; D001672:Biocompatible Materials; D002214:Capsules; D006603:Hexuronic Acids; D020100:Hydrogels; D011092:Polyethylene Glycols; D020723:Glucuronic Acid
Alginate; Capsule; Cirrhosis; Encapsulation; Fibrosis; Hepatitis; Hepatocyte; Hydrogels; Liver failure; Mesenchymal stem cells; Microbeads; Microspheres