Endogenous cannabinoid system alterations and their role in epileptogenesis after brain injury in rat.
Post-traumatic epilepsy (PTE) is one of the most common complications resulting from brain injury, however, antiepileptic drugs usually fail to prevent it. Several lines of evidence have demonstrated that the endogenous cannabinoid system (ECS) plays a pivotal role during epileptogenesis in several animal models. A recent study has shown that a cannabinoid type 1 (CB1) receptor antagonist could suppress long-term neuron hyperexcitability after brain injury, but the underlying mechanisms remain largely unknown. In this study, we first analyzed the dynamic expression of different components of the ECS at various time points after brain injury in rats. Then, we conducted a 12-month-long session of behavioral monitoring after the brain injury, and based on the results, the rats were divided into a PTE group and a non-PTE group. Finally, the changes in the ECS between the two groups were compared. We found that the ECS exhibited a biphasic alteration after brain injury; the expression of the CB1receptor and 2-arachidonoylglycerol (2-AG) in the PTE group was significantly higher than that of the non-PTE group 12 months after traumatic brain injury. Our preliminary results indicated that the ECS might be involved in post-traumatic epileptogenesis.
Department of Neurosurgery, Beijing Tian Tan Hospital, Capital Medical University, Beijing 100050, China; Beijing Key Laboratory of Neurostimulation, Beijing 100050, China.
Xiu
X
Wang
Department of Neurosurgery, Beijing Tian Tan Hospital, Capital Medical University, Beijing 100050, China; Beijing Key Laboratory of Neurostimulation, Beijing 100050, China.
Yao
Y
Wang
Department of Neurosurgery, Beijing Tian Tan Hospital, Capital Medical University, Beijing 100050, China; Beijing Key Laboratory of Neurostimulation, Beijing 100050, China.
Chao
C
Zhang
Department of Neurosurgery, Beijing Tian Tan Hospital, Capital Medical University, Beijing 100050, China; Beijing Key Laboratory of Neurostimulation, Beijing 100050, China.
Chang
C
Liu
Beijing Jingmei Group General Hospital, Beijing 102300, China.
Hai-Feng
HF
Yang
Beijing Key Laboratory of Neurostimulation, Beijing 100050, China; Stereotactic and Functional Neurosurgery Laboratory, Beijing Neurosurgical Institute, Capital Medical University, Beijing 100050, China; Epilepsy Center, Medical Alliance of Beijing Tian Tan Hospital, Peking University First Hospital Fengtai Hospital, Beijing 100050, China.
Wen-Han
WH
Hu
Department of Neurosurgery, Beijing Tian Tan Hospital, Capital Medical University, Beijing 100050, China; Beijing Key Laboratory of Neurostimulation, Beijing 100050, China; Stereotactic and Functional Neurosurgery Laboratory, Beijing Neurosurgical Institute, Capital Medical University, Beijing 100050, China; Epilepsy Center, Medical Alliance of Beijing Tian Tan Hospital, Peking University First Hospital Fengtai Hospital, Beijing 100050, China.
Jian-Guo
JG
Zhang
Department of Neurosurgery, Beijing Tian Tan Hospital, Capital Medical University, Beijing 100050, China; Beijing Key Laboratory of Neurostimulation, Beijing 100050, China; Stereotactic and Functional Neurosurgery Laboratory, Beijing Neurosurgical Institute, Capital Medical University, Beijing 100050, China; Epilepsy Center, Medical Alliance of Beijing Tian Tan Hospital, Peking University First Hospital Fengtai Hospital, Beijing 100050, China. Electronic address: zhangkai62035@163.com.
Kai
K
Zhang
D000818:Animals; D001095:Arachidonic Acids / Q000378:metabolism; D015153:Blotting, Western; D001930:Brain Injuries / Q000150:complications* / Q000378:metabolism*; D004195:Disease Models, Animal; D018450:Disease Progression; D000069280:Electrocorticography; D063388:Endocannabinoids / Q000378:metabolism*; D004834:Epilepsy, Post-Traumatic / Q000209:etiology / Q000378:metabolism*; D015870:Gene Expression / Q000502:physiology; D005989:Glycerides / Q000378:metabolism; D006624:Hippocampus / Q000378:metabolism; D008297:Male; D012333:RNA, Messenger / Q000378:metabolism; D017207:Rats, Sprague-Dawley; D060888:Real-Time Polymerase Chain Reaction; D043884:Receptor, Cannabinoid, CB1 / Q000378:metabolism
D001095:Arachidonic Acids; C543264:Cnr1 protein, rat; D063388:Endocannabinoids; D005989:Glycerides; D012333:RNA, Messenger; D043884:Receptor, Cannabinoid, CB1; C094503:glyceryl 2-arachidonate
CB(1) receptor; Cannabinoid; Epileptogenesis; Post-traumatic epilepsy