Enhanced electrogenerated chemiluminescence behavior of C3N4QDs@ C3N4nanosheet and its signal-on aptasensing for platelet derived growth factor.
A novel g-C3N4nanosheets embedded with C3N4QDs nanocomposites (QD@CNNS) was prepared by simple oxidation using hydrogen peroxide and UV light irradiation. This nanocomposite exhibits more stable and stronger electrochemiluminescent (ECL) behavior compared with CNNS. Coupling this nanocomposite with Fc-labeled aptamer, a signal-on aptasensor for platelet derived growth factor BB (PDGF-BB) is fabricated. Initially, the Fc-labeled aptamer binds onto QD@CNNS via π-π conjugation and electrostatic interaction, quenching ECL emission from QD@CNNS. The introduction of target efficiently recovers the ECL signal by the formation of PDGF-BB/aptamer complex. The ECL intensity is proportion to the concentration of PDGF-BB in the range of 0.02-80nM with a detection limit of 0.013nM. This work demonstrates a simple synthesis method to obtain QD@CNNS with excellent ECL behavior, and opens up the application of g-C3N4nanocomposite in signal-on aptasensing.
MOE Key Laboratory of Analysis and Detection for Food Safety, State Key Laboratory of Photo catalysis on Energy and Environment, and College of Chemistry, Fuzhou University, Fujian 350108, PR China; Academy of Integrative Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou, Fujian 350122, PR China.
Huifeng
H
Xu
Department of Environmental Science and Engineering, Fuzhou University, Minhou, Fujian 350108, PR China.
Shijing
S
Liang
College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, PR China.
Xi
X
Zhu
Department of Environmental Science and Engineering, Fuzhou University, Minhou, Fujian 350108, PR China.
Xiuqin
X
Wu
MOE Key Laboratory of Analysis and Detection for Food Safety, State Key Laboratory of Photo catalysis on Energy and Environment, and College of Chemistry, Fuzhou University, Fujian 350108, PR China.
Yongqiang
Y
Dong
MOE Key Laboratory of Analysis and Detection for Food Safety, State Key Laboratory of Photo catalysis on Energy and Environment, and College of Chemistry, Fuzhou University, Fujian 350108, PR China.
Haishan
H
Wu
Academy of Integrative Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou, Fujian 350122, PR China.
Wenxia
W
Zhang
MOE Key Laboratory of Analysis and Detection for Food Safety, State Key Laboratory of Photo catalysis on Energy and Environment, and College of Chemistry, Fuzhou University, Fujian 350108, PR China. Electronic address: y.w.chi@fzu.edu.cn.
Yuwu
Y
Chi
D052157:Aptamers, Nucleotide / Q000737:chemistry*; D000077214:Becaplermin; D015374:Biosensing Techniques / Q000379:methods*; D055664:Electrochemical Techniques / Q000379:methods; D006801:Humans; D057230:Limit of Detection; D008163:Luminescent Measurements / Q000379:methods; D053761:Nanocomposites / Q000737:chemistry / Q000648:ultrastructure; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure; D009570:Nitriles / Q000737:chemistry*; D020574:Proto-Oncogene Proteins c-sis / Q000097:blood*; D045663:Quantum Dots / Q000737:chemistry* / Q000648:ultrastructure
D052157:Aptamers, Nucleotide; D009570:Nitriles; D020574:Proto-Oncogene Proteins c-sis; D000077214:Becaplermin; C011206:cyanogen
C(3)N(4) QDs@ C(3)N(4) nanosheet; Electrochemiluminescent; Platelet derived growth factor BB; Signal-on aptasensor