Design of Tetra-arm PEG-crosslinked Thermoresponsive Hydrogel for 3D Cell Culture.
We synthesized poly(N-isopropylacrylamide) gel containing cell-adhesive peptides, RGDS pendants, and crosslinked by a hydrophilic polymer. Since gelation occurs by simply mixing the polymers, cells can be simultaneously encapsulated inside the gel during gelation. This gel has roughly a 15% volume change between 25 and 37°C, and is transparent at both temperatures. Moreover, adhesion of encapsulated C2C12 cells to the gel could be observed. This thermoresponsive gel would be potentially useful as a cell culture material that can control cell fate by changing mechanical properties of the cell's external environment.
Department of Materials Engineering, Graduate School of Engineering, The University of Tokyo.
Aya Mizutani
AM
Akimoto
Erika
E
Hasuike
Hiroto
H
Tada
Kenichi
K
Nagase
Teruo
T
Okano
Hideko
H
Kanazawa
Ryo
R
Yoshida
D000178:Acrylamides / Q000737:chemistry*; D000180:Acrylic Resins / Q000737:chemistry; D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods*; D019070:Cell Lineage; D002470:Cell Survival; D002478:Cells, Cultured; D020100:Hydrogels / Q000737:chemistry*; D057927:Hydrophobic and Hydrophilic Interactions; D008422:Materials Testing; D051379:Mice; D010455:Peptides / Q000737:chemistry; D011092:Polyethylene Glycols / Q000737:chemistry*; D013314:Stress, Mechanical; D013696:Temperature
D000178:Acrylamides; D000180:Acrylic Resins; D020100:Hydrogels; D010455:Peptides; C052970:poly-N-isopropylacrylamide; D011092:Polyethylene Glycols; C067295:N-isopropylacrylamide