Novel nanofibrous dressings containing rhEGF and Aloe vera for wound healing applications.
Nanofibrous membranes produced by electrospinning possess a large surface area-to-volume ratio, which mimics the three-dimensional structure of the extracellular matrix. Thus, nanofibrous dressings are a promising alternative for chronic wound healing, since they can replace the natural ECM until it is repaired. Therefore, in this study we have developed a PLGA nanofibrous membrane that contains recombinant human Epidermal Growth Factor (rhEGF) and Aloe vera (AV) extract. Both of them promote wound healing, as EGF is a wound healing mediator and AV stimulates the proliferation and activity of fibroblast. The obtained membranes were composed of uniform and randomly oriented fibers with an average diameter of 356.03±112.05nm, they presented a porosity of 87.92±11.96% and the amount of rhEGF was 9.76±1.75μg/mg. The in vitro viability assay demonstrated that the membranes containing rhEGF and AV improved fibroblast proliferation, revealing the beneficial effect of the combination. Furthermore, these membranes accelerated significantly wound closure and reepithelisation in an in vivo full thickness wound healing assay carried out in db/db mice. Overall, these findings demonstrated the potential of PLGA nanofibers containing rhEGF and AV for the treatment of chronic wounds.
NanoBioCel Group, Laboratory of Pharmaceutics, School of Pharmacy, University of the Basque Country (UPV/EHU), Vitoria-Gasteiz, Spain.
Itxaso
I
Garcia-Orue
NanoBioCel Group, Laboratory of Pharmaceutics, School of Pharmacy, University of the Basque Country (UPV/EHU), Vitoria-Gasteiz, Spain.
Garazi
G
Gainza
Hospital Universitario de Álava (HUA) Txagorritxu, Vitoria-Gasteiz, 01009, Spain.
Franciso Borja
FB
Gutierrez
Hospital Universitario de Álava (HUA) Txagorritxu, Vitoria-Gasteiz, 01009, Spain.
Jose Javier
JJ
Aguirre
Department of Chemical Engineering and Pharmaceutical Technology, School of Pharmacy, Institute of Biomedical Technologies (ITB), Center for Biomedical Research of the Canary Islands (CIBICAN), University of La Laguna, Tenerife, Spain.
Carmen
C
Evora
NanoBioCel Group, Laboratory of Pharmaceutics, School of Pharmacy, University of the Basque Country (UPV/EHU), Vitoria-Gasteiz, Spain; Biomedical Research Networking Centre in Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), Vitoria-Gasteiz, Spain.
Jose Luis
JL
Pedraz
NanoBioCel Group, Laboratory of Pharmaceutics, School of Pharmacy, University of the Basque Country (UPV/EHU), Vitoria-Gasteiz, Spain; Biomedical Research Networking Centre in Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), Vitoria-Gasteiz, Spain.
Rosa Maria
RM
Hernandez
Department of Chemical Engineering and Pharmaceutical Technology, School of Pharmacy, Institute of Biomedical Technologies (ITB), Center for Biomedical Research of the Canary Islands (CIBICAN), University of La Laguna, Tenerife, Spain. Electronic address: adelgado@ull.es.
Araceli
A
Delgado
NanoBioCel Group, Laboratory of Pharmaceutics, School of Pharmacy, University of the Basque Country (UPV/EHU), Vitoria-Gasteiz, Spain; Biomedical Research Networking Centre in Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), Vitoria-Gasteiz, Spain. Electronic address: manoli.igartua@ehu.eus.
Manoli
M
Igartua
D000504:Aloe / Q000737:chemistry*; D000818:Animals; D001458:Bandages; D004815:Epidermal Growth Factor / Q000494:pharmacology*; D006801:Humans; D051379:Mice; D057139:Nanofibers; D011994:Recombinant Proteins / Q000494:pharmacology; D014945:Wound Healing
D011994:Recombinant Proteins; D004815:Epidermal Growth Factor
Aloe vera; Electrospinning; Growth factors; Wound dressing; Wound healing; rhEGF