Metabolites from the co-culture of nigranoic acid and Umbelopsis dimorpha SWUKD3.1410, an endophytic fungus from Kadsura angustifolia.
The ability of the endophytic fungus Umbelopsis dimorpha SWUKD3.1410 to transform the triterpene nigranoic acid (3, 4-secocycloarta-4(28), 24-(Z)-diene-3, 26-dioic acid; 1) was investigated. Co-culture of nigranoic acid with U. dimorpha SWUKD3.1410 yielded six major products, including a new triterpene (2) and 5 known compounds (3-7). Their structures were identified by the extensive 1D, 2D NMR and HR-ESI-MS Data analysis, and by comparison with those reported data. Among them, 2-4 were transformed from nigranoic acid (1), while 5-7 were derived from the culture medium stimulated by the substrate. On the basis of the structures of 2-4, it was proposed that the transformative process probably involved isomerase and hydroxylase, in which the former was rare in fungi. Additionally, all the compounds (1-7) were evaluated for their cytotoxic activity. As a result, compounds 1, 3-5 exhibited weak cytotoxicity against the tested cell lines, while others showed no cytotoxicity.
a Key Laboratory of Eco-environments in Three Gorges Reservoir Region, Ministry of Education, School of Life Sciences , Southwest University , Chongqing , People's Republic of China.
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Wang
a Key Laboratory of Eco-environments in Three Gorges Reservoir Region, Ministry of Education, School of Life Sciences , Southwest University , Chongqing , People's Republic of China.
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D
Qin
a Key Laboratory of Eco-environments in Three Gorges Reservoir Region, Ministry of Education, School of Life Sciences , Southwest University , Chongqing , People's Republic of China.
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K
Zhang
a Key Laboratory of Eco-environments in Three Gorges Reservoir Region, Ministry of Education, School of Life Sciences , Southwest University , Chongqing , People's Republic of China.
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Huang
a Key Laboratory of Eco-environments in Three Gorges Reservoir Region, Ministry of Education, School of Life Sciences , Southwest University , Chongqing , People's Republic of China.
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S
Liu
a Key Laboratory of Eco-environments in Three Gorges Reservoir Region, Ministry of Education, School of Life Sciences , Southwest University , Chongqing , People's Republic of China.
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MJ
Han
a Key Laboratory of Eco-environments in Three Gorges Reservoir Region, Ministry of Education, School of Life Sciences , Southwest University , Chongqing , People's Republic of China.
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JY
Dong
D000970:Antineoplastic Agents / Q000494:pharmacology; D045744:Cell Line, Tumor; D018920:Coculture Techniques; D060026:Endophytes / Q000378:metabolism*; D006801:Humans; D032135:Kadsura / Q000382:microbiology*; D009682:Magnetic Resonance Spectroscopy; D009090:Mucorales / Q000378:metabolism*; D014315:Triterpenes / Q000378:metabolism*
D000970:Antineoplastic Agents; D014315:Triterpenes; C100860:nigranoic acid
Endophytic fungus; Umbelopsis dimorpha; cytotoxicity; microbial transformation; nigranoic acid; structural identification