Creation of Three-Dimensional Liver Tissue Models from Experimental Images for Systems Medicine.
In this chapter, we illustrate how three-dimensional liver tissue models can be created from experimental image modalities by utilizing a well-established processing chain of experiments, microscopic imaging, image processing, image analysis and model construction. We describe how key features of liver tissue architecture are quantified and translated into model parameterizations, and show how a systematic iteration of experiments and model simulations often leads to a better understanding of biological phenomena in systems biology and systems medicine.
Institute for Computer Science, University of Leipzig, Haertelstraße 16-18, 04107, Leipzig, Germany. hoehme@uni-leipzig.de.
Stefan
S
Hoehme
Institute for Computer Science, University of Leipzig, Haertelstraße 16-18, 04107, Leipzig, Germany.
Adrian
A
Friebel
Molecular Hepatology, Department of Medicine II, Medical Faculty Mannheim, University of Heidelberg, Mannheim, Germany.|Department of Forensic Medicine and Veterinary Toxicology, Faculty of Veterinary Medicine, South Valley University, Qena, Egypt.
Seddik
S
Hammad
INRIA, Unit Rocquencourt B.P.105, 78153 Le Chesnay Cedex-France, Laboratoire Jacques-Louis Lions, France Université of Paris 06, CNRS UMR 7598, 4 pl. Jussieu, Paris, France.
Dirk
D
Drasdo
Leibniz Research Centre for Working Environment and Human Factors (IfADo), TU Dortmund University, Dortmund, Germany.
Jan G
JG
Hengstler
D000818:Animals; D005456:Fluorescent Dyes / Q000737:chemistry; D021621:Imaging, Three-Dimensional / Q000379:methods*; D007150:Immunohistochemistry / Q000295:instrumentation / Q000379:methods; D008099:Liver / Q000033:anatomy & histology / Q000000981:diagnostic imaging* / Q000473:pathology; D051379:Mice; D018345:Mice, Knockout; D018613:Microscopy, Confocal / Q000295:instrumentation / Q000379:methods*; D008954:Models, Biological; D011786:Quality Control; D012984:Software; D013194:Staining and Labeling / Q000295:instrumentation / Q000379:methods*; D013552:Swine
D005456:Fluorescent Dyes
2D/3D microscopy; Confocal scanning microscopy; Hepatocyte transplantation; Liver architecture; Liver tissue model; Spatiotemporal model; Systems biology; Systems medicine; TiQuant software