Optimization of industrial microorganisms: recent advances in synthetic dynamic regulators.
Production of biochemicals by industrial fermentation using microorganisms requires maintaining cellular production capacity, because maximal productivity is economically important. High-productivity microbial strains can be developed using static engineering, but these may not maintain maximal productivity throughout the culture period as culture conditions and cell states change dynamically. Additionally, economic reasons limit heterologous protein expression using inducible promoters to prevent metabolic burden for commodity chemical and biofuel production. Recently, synthetic and systems biology has been used to design genetic circuits, precisely controlling gene expression or influencing genetic behavior toward a desired phenotype. Development of dynamic regulators can maintain cellular phenotype in a maximum production state in response to factors including cell concentration, oxygen, temperature, pH, and metabolites. Herein, we introduce dynamic regulators of industrial microorganism optimization and discuss metabolic flux fine control by dynamic regulators in response to metabolites or extracellular stimuli, robust production systems, and auto-induction systems using quorum sensing.
Department of Chemical Engineering, Pohang University of Science and Technology, 77 Cheongam-ro, Nam-gu, Pohang, Gyeongbuk, 37673, Korea.
Byung Eun
BE
Min
School of Interdisciplinary Bioscience and Bioengineering, Pohang University of Science and Technology, 77 Cheongam-ro, Nam-gu, Pohang, Gyeongbuk, 37673, Korea.
Hyun Gyu
HG
Hwang
Department of Chemical Engineering, Pohang University of Science and Technology, 77 Cheongam-ro, Nam-gu, Pohang, Gyeongbuk, 37673, Korea.
Hyun Gyu
HG
Lim
Department of Chemical Engineering, Pohang University of Science and Technology, 77 Cheongam-ro, Nam-gu, Pohang, Gyeongbuk, 37673, Korea. gyjung@postech.ac.kr.|School of Interdisciplinary Bioscience and Bioengineering, Pohang University of Science and Technology, 77 Cheongam-ro, Nam-gu, Pohang, Gyeongbuk, 37673, Korea. gyjung@postech.ac.kr.
Gyoo Yeol
GY
Jung
D056804:Biofuels; D015870:Gene Expression; D006863:Hydrogen-Ion Concentration; D007218:Industrial Microbiology; D060847:Metabolic Engineering; D010100:Oxygen / Q000737:chemistry; D010641:Phenotype; D011401:Promoter Regions, Genetic; D053038:Quorum Sensing; D058615:Synthetic Biology; D049490:Systems Biology / Q000379:methods*; D013696:Temperature
D056804:Biofuels; D010100:Oxygen
Dynamic regulator; Industrial microorganism; Quorum sensing; Synthetic biology; Transcription factors