Expression of senescence-associated genes in multipotent mesenchymal stromal cells during long-term cultivation at various hypoxic levels.
The expression of several genes which functions are associated with cellular senescence was analyzed in multipotent mesenchymal stromal cells during long-term cultivation at different oxygen levels (20, 5, and 1%) using the RT² Profiler™ PCR Array Human Cellular Senescence system (Qiagen, United States). It was established that replicative senescence processes develop most actively in the cells cultured under the standard conditions (20% O2). The most significant changes were observed in the expression of CCND1, ID1, IGF1, PIK3CA, and SERPINE1 genes.
Institute of Biomedical Problems, Russian Academy of Sciences, Khoroshevskoe sh. 76-a, Moscow, 123007, Russia. pogodina_m@mail.ru.
M V
MV
Lobanova
Institute of Biomedical Problems, Russian Academy of Sciences, Khoroshevskoe sh. 76-a, Moscow, 123007, Russia.
A Y
AY
Ratushnyy
Institute of Biomedical Problems, Russian Academy of Sciences, Khoroshevskoe sh. 76-a, Moscow, 123007, Russia.
L B
LB
Buravkova
D000273:Adipose Tissue / Q000378:metabolism; D015687:Cell Hypoxia / Q000502:physiology*; D002478:Cells, Cultured; D016922:Cellular Senescence / Q000502:physiology*; D058534:Class I Phosphatidylinositol 3-Kinases; D019938:Cyclin D1 / Q000378:metabolism; D051766:Early Growth Response Protein 1 / Q000378:metabolism; D005136:Eye Proteins / Q000378:metabolism; D015870:Gene Expression; D000071679:Glycogen Synthase Kinase 3 beta / Q000378:metabolism; D006801:Humans; D051798:Inhibitor of Differentiation Protein 1 / Q000378:metabolism; D007334:Insulin-Like Growth Factor I / Q000378:metabolism; D059630:Mesenchymal Stem Cells / Q000378:metabolism*; D009414:Nerve Growth Factors / Q000378:metabolism; D019869:Phosphatidylinositol 3-Kinases / Q000378:metabolism; D016133:Polymerase Chain Reaction; D051736:Proto-Oncogene Proteins c-mdm2 / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D015843:Serpins / Q000378:metabolism; D000099174:Pigment Epithelium-Derived Factor
D058534:Class I Phosphatidylinositol 3-Kinases; D019938:Cyclin D1; D051766:Early Growth Response Protein 1; D005136:Eye Proteins; D000071679:Glycogen Synthase Kinase 3 beta; D051798:Inhibitor of Differentiation Protein 1; D007334:Insulin-Like Growth Factor I; D009414:Nerve Growth Factors; D019869:Phosphatidylinositol 3-Kinases; D051736:Proto-Oncogene Proteins c-mdm2; D012333:RNA, Messenger; D015843:Serpins; D000099174:Pigment Epithelium-Derived Factor; C530104:CCND1 protein, human; C497325:EGR1 protein, human; C497453:ID1 protein, human; C583557:IGF1 protein, human; C497206:MDM2 protein, human; C484760:PIK3CA protein, human; C000605506:GSK3B protein, human