A chemical reporter strategy for detecting and identifying O-mycoloylated proteins in Corynebacterium.
Protein O-mycoloylation is a unique post-translational lipidation that was recently discovered in Corynebacterium. We describe an alkyne-modified trehalose monomycolate chemical reporter that can metabolically tag O-mycoloylated proteins in C. glutamicum, enabling their detection and identification through click chemistry.
Department of Chemistry and Biochemistry, Central Michigan University, Mount Pleasant, MI 48859, USA. ben.swarts@cmich.edu.
Herbert W
HW
Kavunja
Department of Chemistry and Biochemistry, Central Michigan University, Mount Pleasant, MI 48859, USA. ben.swarts@cmich.edu.
Brent F
BF
Piligian
Department of Chemistry and Biochemistry, Central Michigan University, Mount Pleasant, MI 48859, USA. ben.swarts@cmich.edu.
Taylor J
TJ
Fiolek
Department of Chemistry and Biochemistry, Central Michigan University, Mount Pleasant, MI 48859, USA. ben.swarts@cmich.edu.
Hannah N
HN
Foley
Department of Chemistry and Biochemistry, Central Michigan University, Mount Pleasant, MI 48859, USA. ben.swarts@cmich.edu.
Temitope O
TO
Nathan
Department of Chemistry and Biochemistry, Central Michigan University, Mount Pleasant, MI 48859, USA. ben.swarts@cmich.edu.
Benjamin M
BM
Swarts
D000480:Alkynes / Q000737:chemistry / Q000378:metabolism*; D001426:Bacterial Proteins / Q000032:analysis* / Q000378:metabolism; D057930:Click Chemistry; D003311:Cord Factors / Q000737:chemistry / Q000378:metabolism*; D003352:Corynebacterium / Q000737:chemistry* / Q000378:metabolism; D015394:Molecular Structure; D011499:Protein Processing, Post-Translational
D000480:Alkynes; D001426:Bacterial Proteins; D003311:Cord Factors; C021099:trehalose monomycolate