Regulation of Vegetative Phase Change by SWI2/SNF2 Chromatin Remodeling ATPase BRAHMA.
Plants progress from a juvenile vegetative phase of development to an adult vegetative phase of development before they enter the reproductive phase. miR156 has been shown to be the master regulator of the juvenile-to-adult transition in plants. However, the mechanism of how miR156 is transcriptionally regulated still remains elusive. In a forward genetic screen, we identified that a mutation in the SWI2/SNF2 chromatin remodeling ATPase BRAHMA (BRM) exhibited an accelerated vegetative phase change phenotype by reducing the expression of miR156, which in turn caused a corresponding increase in the levels of SQUAMOSA PROMOTER BINDING PROTEIN LIKE genes. BRM regulates miR156 expression by directly binding to the MIR156A promoter. Mutations in BRM not only increased occupancy of the -2 and +1 nucleosomes proximal to the transcription start site at the MIR156A locus but also the levels of trimethylated histone H3 at Lys 27. The precocious phenotype of brm mutant was partially suppressed by a second mutation in SWINGER (SWN), but not by a mutation in CURLEY LEAF, both of which are key components of the Polycomb Group Repressive Complex 2 in plants. Our results indicate that BRM and SWN act antagonistically at the nucleosome level to fine-tune the temporal expression of miR156 to regulate vegetative phase change in Arabidopsis.
Zhejiang Provincial Key Laboratory of Bioremediation of Soil Contamination, Laboratory of Plant Molecular and Developmental Biology, Zhejiang Agriculture and Forestry University, Hangzhou 311300, China (Y.X., C.G., B. Zhou., H.W., B. Zheng, H.D., Z.Z., G.W.).|Agriculture and Agri-Food Canada, London Research and Development Centre, London, Ontario, N5V 4T3, Canada (C.L., Y.C.); and.|Department of Biology, University of Pennsylvania, Philadelphia, Pennsylvania 19104 (A.P., S.P.).
Yunmin
Y
Xu
Zhejiang Provincial Key Laboratory of Bioremediation of Soil Contamination, Laboratory of Plant Molecular and Developmental Biology, Zhejiang Agriculture and Forestry University, Hangzhou 311300, China (Y.X., C.G., B. Zhou., H.W., B. Zheng, H.D., Z.Z., G.W.).|Agriculture and Agri-Food Canada, London Research and Development Centre, London, Ontario, N5V 4T3, Canada (C.L., Y.C.); and.|Department of Biology, University of Pennsylvania, Philadelphia, Pennsylvania 19104 (A.P., S.P.).
Changkui
C
Guo
Zhejiang Provincial Key Laboratory of Bioremediation of Soil Contamination, Laboratory of Plant Molecular and Developmental Biology, Zhejiang Agriculture and Forestry University, Hangzhou 311300, China (Y.X., C.G., B. Zhou., H.W., B. Zheng, H.D., Z.Z., G.W.).|Agriculture and Agri-Food Canada, London Research and Development Centre, London, Ontario, N5V 4T3, Canada (C.L., Y.C.); and.|Department of Biology, University of Pennsylvania, Philadelphia, Pennsylvania 19104 (A.P., S.P.).
Bingying
B
Zhou
Zhejiang Provincial Key Laboratory of Bioremediation of Soil Contamination, Laboratory of Plant Molecular and Developmental Biology, Zhejiang Agriculture and Forestry University, Hangzhou 311300, China (Y.X., C.G., B. Zhou., H.W., B. Zheng, H.D., Z.Z., G.W.).|Agriculture and Agri-Food Canada, London Research and Development Centre, London, Ontario, N5V 4T3, Canada (C.L., Y.C.); and.|Department of Biology, University of Pennsylvania, Philadelphia, Pennsylvania 19104 (A.P., S.P.).
Chenlong
0000-0003-3405-6056
C
Li
Zhejiang Provincial Key Laboratory of Bioremediation of Soil Contamination, Laboratory of Plant Molecular and Developmental Biology, Zhejiang Agriculture and Forestry University, Hangzhou 311300, China (Y.X., C.G., B. Zhou., H.W., B. Zheng, H.D., Z.Z., G.W.).|Agriculture and Agri-Food Canada, London Research and Development Centre, London, Ontario, N5V 4T3, Canada (C.L., Y.C.); and.|Department of Biology, University of Pennsylvania, Philadelphia, Pennsylvania 19104 (A.P., S.P.).
Huasen
H
Wang
Zhejiang Provincial Key Laboratory of Bioremediation of Soil Contamination, Laboratory of Plant Molecular and Developmental Biology, Zhejiang Agriculture and Forestry University, Hangzhou 311300, China (Y.X., C.G., B. Zhou., H.W., B. Zheng, H.D., Z.Z., G.W.).|Agriculture and Agri-Food Canada, London Research and Development Centre, London, Ontario, N5V 4T3, Canada (C.L., Y.C.); and.|Department of Biology, University of Pennsylvania, Philadelphia, Pennsylvania 19104 (A.P., S.P.).
Ben
0000-0002-8542-8465
B
Zheng
Zhejiang Provincial Key Laboratory of Bioremediation of Soil Contamination, Laboratory of Plant Molecular and Developmental Biology, Zhejiang Agriculture and Forestry University, Hangzhou 311300, China (Y.X., C.G., B. Zhou., H.W., B. Zheng, H.D., Z.Z., G.W.).|Agriculture and Agri-Food Canada, London Research and Development Centre, London, Ontario, N5V 4T3, Canada (C.L., Y.C.); and.|Department of Biology, University of Pennsylvania, Philadelphia, Pennsylvania 19104 (A.P., S.P.).
Han
H
Ding
Zhejiang Provincial Key Laboratory of Bioremediation of Soil Contamination, Laboratory of Plant Molecular and Developmental Biology, Zhejiang Agriculture and Forestry University, Hangzhou 311300, China (Y.X., C.G., B. Zhou., H.W., B. Zheng, H.D., Z.Z., G.W.).|Agriculture and Agri-Food Canada, London Research and Development Centre, London, Ontario, N5V 4T3, Canada (C.L., Y.C.); and.|Department of Biology, University of Pennsylvania, Philadelphia, Pennsylvania 19104 (A.P., S.P.).
Zhujun
Z
Zhu
Zhejiang Provincial Key Laboratory of Bioremediation of Soil Contamination, Laboratory of Plant Molecular and Developmental Biology, Zhejiang Agriculture and Forestry University, Hangzhou 311300, China (Y.X., C.G., B. Zhou., H.W., B. Zheng, H.D., Z.Z., G.W.).|Agriculture and Agri-Food Canada, London Research and Development Centre, London, Ontario, N5V 4T3, Canada (C.L., Y.C.); and.|Department of Biology, University of Pennsylvania, Philadelphia, Pennsylvania 19104 (A.P., S.P.).
Angela
A
Peragine
Zhejiang Provincial Key Laboratory of Bioremediation of Soil Contamination, Laboratory of Plant Molecular and Developmental Biology, Zhejiang Agriculture and Forestry University, Hangzhou 311300, China (Y.X., C.G., B. Zhou., H.W., B. Zheng, H.D., Z.Z., G.W.).|Agriculture and Agri-Food Canada, London Research and Development Centre, London, Ontario, N5V 4T3, Canada (C.L., Y.C.); and.|Department of Biology, University of Pennsylvania, Philadelphia, Pennsylvania 19104 (A.P., S.P.).
Yuhai
0000-0002-3675-3288
Y
Cui
Zhejiang Provincial Key Laboratory of Bioremediation of Soil Contamination, Laboratory of Plant Molecular and Developmental Biology, Zhejiang Agriculture and Forestry University, Hangzhou 311300, China (Y.X., C.G., B. Zhou., H.W., B. Zheng, H.D., Z.Z., G.W.).|Agriculture and Agri-Food Canada, London Research and Development Centre, London, Ontario, N5V 4T3, Canada (C.L., Y.C.); and.|Department of Biology, University of Pennsylvania, Philadelphia, Pennsylvania 19104 (A.P., S.P.).
Scott
0000-0001-6592-5862
S
Poethig
Zhejiang Provincial Key Laboratory of Bioremediation of Soil Contamination, Laboratory of Plant Molecular and Developmental Biology, Zhejiang Agriculture and Forestry University, Hangzhou 311300, China (Y.X., C.G., B. Zhou., H.W., B. Zheng, H.D., Z.Z., G.W.); wugang@zafu.edu.cn.|Agriculture and Agri-Food Canada, London Research and Development Centre, London, Ontario, N5V 4T3, Canada (C.L., Y.C.); and wugang@zafu.edu.cn.|Department of Biology, University of Pennsylvania, Philadelphia, Pennsylvania 19104 (A.P., S.P.) wugang@zafu.edu.cn.
Gang
0000-0002-6876-3886
G
Wu
D000251:Adenosine Triphosphatases / Q000235:genetics / Q000378:metabolism*; D017360:Arabidopsis / Q000254:growth & development* / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D042002:Chromatin Assembly and Disassembly; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D056426:Genetic Loci; D006657:Histones / Q000378:metabolism; D018398:Homeodomain Proteins / Q000378:metabolism; D008239:Lysine / Q000378:metabolism; D008745:Methylation; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism; D008954:Models, Biological; D009154:Mutation / Q000235:genetics; D009707:Nucleosomes / Q000378:metabolism; D010641:Phenotype; D011401:Promoter Regions, Genetic / Q000235:genetics; D011485:Protein Binding
D029681:Arabidopsis Proteins; C104597:CURLY LEAF protein, Arabidopsis; D006657:Histones; D018398:Homeodomain Proteins; D035683:MicroRNAs; D009707:Nucleosomes; D000251:Adenosine Triphosphatases; C514541:BRM protein, Arabidopsis; D008239:Lysine