Cytotoxic T-lymphocyte-associated protein 4 gene polymorphism is related to rheumatoid arthritis in Egyptian population.
BACKGROUND
Cytotoxic T-lymphocyte-associated protein 4 (CTLA-4) is a CD28-family receptor expressed on T-cells which suppresses T cell proliferation. CTLA-4 -318C/T polymorphism is involved in regulation of CTLA-4 expression.


OBJECTIVE
The study aimed to investigate the genetic association of CTLA-4 -318C/T polymorphism with rheumatoid arthritis (RA) and the activity and severity of the disease in the Egyptian population.


METHODS
A single nucleotide polymorphism (rs5742909) in CTLA-4 was genotyped in 100 RA patients and 100 healthy controls using polymerase chain reaction-restriction fragment length polymorphism. Diagnostic tests were measured for RA patients.


RESULTS
The frequency of T allele in RA patients was significantly higher than in the control subjects (p = 0.002). CT and TT genotypes had high C-reactive protein, erythrocyte sedimentation rate and disease activity score 28 while CC genotype had a high rheumatoid factor.


CONCLUSIONS
A minor allele of CTLA-4 rs5742909 polymorphism was associated with RA and the activity but not the severity of the disease.
a Department of Biochemistry , Faculty of Pharmacy, Suez Canal University , Ismailia , Egypt and.
Shaimaa A
SA
Fattah
b Department of Medical Biochemistry , Faculty of Medicine, Port Said University , Port Said , Egypt.
Maivel H
MH
Ghattas
a Department of Biochemistry , Faculty of Pharmacy, Suez Canal University , Ismailia , Egypt and.
Samy M
SM
Saleh
a Department of Biochemistry , Faculty of Pharmacy, Suez Canal University , Ismailia , Egypt and.
Dina M
DM
Abo-Elmatty
D000328:Adult; D000483:Alleles; D001172:Arthritis, Rheumatoid / Q000097:blood / Q000235:genetics* / Q000378:metabolism / Q000503:physiopathology; D001799:Blood Sedimentation; D002097:C-Reactive Protein / Q000032:analysis; D060908:CTLA-4 Antigen / Q000235:genetics* / Q000378:metabolism; D016022:Case-Control Studies; D004534:Egypt; D005260:Female; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease; D006579:Heterozygote; D006785:Hospitals, University; D006801:Humans; D008297:Male; D008875:Middle Aged; D010044:Outpatient Clinics, Hospital; D020641:Polymorphism, Single Nucleotide; D011401:Promoter Regions, Genetic; D012217:Rheumatoid Factor / Q000097:blood; D012720:Severity of Illness Index
D060908:CTLA-4 Antigen; C556706:CTLA4 protein, human; D002097:C-Reactive Protein; D012217:Rheumatoid Factor
CTLA-4; rheumatoid arthritis; rs5742909 polymorphism