Development of a Targeted anti-HER2 scFv Chimeric Peptide for Gene Delivery into HER2-Positive Breast Cancer Cells.
Chimeric polymers are known as suitable carriers for gene delivery. Certain properties are critical for a polymer to be used as a gene delivery vector. A new polymer was designed for the targeted delivery of genes into breast cancer cell lines, based on MPG peptide. It is composed of different functional domains, including HIV gp41, nuclear localization sequence of SV40 T-antigen, two C-terminus repeats of histone H1, and the scFv of anti-HER2 antibody. The results demonstrated that the vector can effectively condense plasmid DNA into nanoparticles with an average size of 250nm. Moreover, fusion of the scFv portion to the carrier brought about the specific recognition of HER2. Overall, the transfection efficiency of the vector demonstrated that it could deliver the desired gene into BT-474 HER2-positive breast cancer cells.
Department of Nanobiotechnology, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran.
Roya
R
Cheraghi
Nanobiotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran. Electronic address: nazari1980azar@yahoo.com.
Mahboobeh
M
Nazari
Department of Nanobiotechnology, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran.
Mohsen
M
Alipour
Department of Nanobiotechnology, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran.
Asia
A
Majidi
Department of Biochemistry, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran. Electronic address: saman_h@modares.ac.ir.
Saman
S
Hosseinkhani
D001943:Breast Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000628:therapy*; D045744:Cell Line, Tumor; D005260:Female; D018014:Gene Transfer Techniques; D015316:Genetic Therapy / Q000379:methods; D005822:Genetic Vectors / Q000235:genetics; D006801:Humans; D053758:Nanoparticles / Q000737:chemistry; D010316:Particle Size; D010455:Peptides / Q000737:chemistry* / Q000235:genetics*; D010957:Plasmids / Q000235:genetics; D011108:Polymers / Q000737:chemistry; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000235:genetics*; D057127:Single-Chain Antibodies / Q000378:metabolism*; D014162:Transfection / Q000379:methods
D010455:Peptides; D011108:Polymers; D018719:Erb-b2 Receptor Tyrosine Kinases; D057127:Single-Chain Antibodies