Dendrobium officinale Kimura et Migo attenuates diabetic cardiomyopathy through inhibiting oxidative stress, inflammation and fibrosis in streptozotocin-induced mice.
Dendrobium officinale Kimura et Migo (Dendrobium catenatum Lindley), a prized traditional Chinese Medicine, has been used in China and Southeast Asian countries for centuries. The present study was aimed to investigate the effects and the possible mechanisms of the Dendrobium officinale extracts (DOE) on diabetic cardiomyopathy in mice. The diabetic model was induced by intraperitoneal injection of streptozotocin at the dose of 50mg/kg body weight for 5 consecutive days. After 8 weeks treatment of DOE, mice were sacrificed, blood sample and heart tissues were collected. Our results showed that Streptozotocin-induced diabetic model was effectively achieved and serum CK and LDH levels were significantly increased in mice with diabetic cardiomyopathy. Pretreatment with DOE decreased the heart-to-body weight ratio (HW/BW) and showed an evident hypoglycemic effect. DOE pretreatment significantly decreased CK, LDH, TC and TG levels, limited the production of MDA and increased the activities of T-SOD. The histological analysis of Oil red O staining and Sirius red staining showed an obvious amelioration of cardiac injury, inhibition of cardiac lipid accumulation and deposition of collagen when pretreatment with DOE. In addition, Western blot detection and analysis showed that DOE down-regulated the expression of TGF-β, collegan-1, fibronectin, NF-κB, TNF-α and IL-1β. In conclusion, our study suggested that DOE possesses the cardioprotective potential against diabetic cardiomyopathy, which may be due to the inhibition of oxidative stress, cardiac lipid accumulation, pro-inflammatory cytokines and cardiac fibrosis.
College of Pharmaceutical Sciences, Southwest University, Chongqing 400716, China. Electronic address: sunshine9118@163.com.
Zhihao
Z
Zhang
College of Pharmaceutical Sciences, Southwest University, Chongqing 400716, China. Electronic address: Duozhang1023@163.com.
Duoduo
D
Zhang
College of Pharmaceutical Sciences, Southwest University, Chongqing 400716, China. Electronic address: doumeng7768@sina.com.
Mengmeng
M
Dou
College of Pharmaceutical Sciences, Southwest University, Chongqing 400716, China. Electronic address: zb63@163.com.
Zhubo
Z
Li
Department of Neurology, The Ninth People's Hospital of Chongqing, Chongqing 400700, China. Electronic address: zhjzhj121@163.com.
Jie
J
Zhang
College of Pharmaceutical Sciences, Southwest University, Chongqing 400716, China. Electronic address: zhaoxiaoyan67@163.com.
Xiaoyan
X
Zhao
D000818:Animals; D002316:Cardiotonic Agents / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use*; D031667:Dendrobium; D003921:Diabetes Mellitus, Experimental / Q000139:chemically induced / Q000378:metabolism / Q000517:prevention & control*; D058065:Diabetic Cardiomyopathies / Q000139:chemically induced / Q000378:metabolism / Q000517:prevention & control*; D004305:Dose-Response Relationship, Drug; D004365:Drugs, Chinese Herbal / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use*; D005355:Fibrosis / Q000188:drug therapy / Q000378:metabolism; D007249:Inflammation / Q000139:chemically induced / Q000378:metabolism / Q000517:prevention & control; D008297:Male; D051379:Mice; D018384:Oxidative Stress / Q000187:drug effects* / Q000502:physiology; D013311:Streptozocin / Q000633:toxicity
D002316:Cardiotonic Agents; D004365:Drugs, Chinese Herbal; D013311:Streptozocin
Dendrobium officinale; Diabetic cardiomyopathy; Fibrosis; Inflammation; Lipid accumulation; Oxidative stress