Identification ofKlebsiella pneumoniaeCarbapenemase-producingKlebsiella oxytocain Clinical Isolates in Tehran Hospitals, Iran by Chromogenic Medium and Molecular Methods.
OBJECTIVE
Production of carbapenemase, especiallyKlebsiella pneumoniaecarbapenemases (KPC), is one of the antibiotic resistance mechanisms of Enterobacteriaceae such asKlebsiella oxytoca. This study aimed to investigate and identify KPC-producingK. oxytocaisolates using molecular and phenotypic methods.


METHODS
A total of 75 isolates ofK. oxytocawere isolated from various clinical samples, and were verified asK. oxytocaafter performing standard microbiological tests and using a polymerase chain reaction (PCR) method. An antibiotic susceptibility test was performed using a disc diffusion method according to the Clinical and Laboratory Standards Institute guidelines. CHROMagar KPC chromogenic culture media was used to examine and confirm the production of the carbapenemase enzyme inK. oxytocaisolates; in addition, PCR was used to evaluate the presence ofblaKPCgene inK. oxytocastrains.


RESULTS
Of a total of 75K. oxytocaisolates, one multidrug resistant strain was isolated from the urine of a hospitalized woman. This strain was examined to assess its ability to produce carbapenemase enzyme; it produced a colony with a blue metallic color on the CHROMagar KPC chromogenic culture media. In addition, theblaKPCgene was confirmed by PCR. After sequencing, it was confirmed and deposited in GenBank.


CONCLUSIONS
To date, many cases of KPC-producing Enterobacteriaceae, in particularK. pneumoniae, have been reported in different countries; there are also some reports on the identification of KPC-producingK. oxytoca. Therefore, to prevent the outbreak of nosocomial infections, the early detection, control, and prevention of the spread of these strains are of great importance.
Division of Microbiology, Department of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
Majid
M
Validi
Division of Microbiology, Department of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran; Food Microbiology Research Center, Tehran University of Medical Sciences, Tehran, Iran.
Mohammad Mehdi
MM
Soltan Dallal
Division of Microbiology, Department of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran; Food Microbiology Research Center, Tehran University of Medical Sciences, Tehran, Iran.
Masoumeh
M
Douraghi
Lister Laboratory of Microbiology, Tehran, Iran.
Jalil
J
Fallah Mehrabadi
Department of Epidemiology and Biostatistics, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
Abbas
A
Rahimi Foroushani
CHROMagar KPC; Klebsiella oxytoca; blaKPC; polymerase chain reaction