Isolation of a simian immunodeficiency virus from a malbrouck (Chlorocebus cynosuros).
To investigate the diversity of simian immunodeficiency virus (SIV) among nonhuman primates (NHPs) in Zambia, next-generation sequencing was performed to determine the complete genome sequence of a novel SIV recovered by co-culturing African green monkey (AGM) peripheral blood lymphocytes with human CD4+T-cell lines. We report the first described SIV (SIVagmMAL-ZMB) from a malbrouck (Chlorocebus cynosuros). SIVagmMAL-ZMB was detected by real-time PCR analysis of splenic RNA in 3.2% (3/94) of AGMs and was undetectable in baboons (0/105). SIVagmMAL-ZMB possessed <80% nucleotide sequence identity to known SIV isolates and was located basally to vervet monkey SIV strains in all phylogenies.
Global Station for Zoonosis Control, Global Institution for Collaborative Research and Education (GI-CoRE), Hokkaido University, N20, W10, Kita-ku, Sapporo, 001-0020, Japan.|National Virus Reference Laboratory, University College Dublin, Belfield, Dublin 4, Ireland.
Michael
http://orcid.org/0000-0003-0011-6209
M
Carr
Division of Molecular Pathobiology, Research Center for Zoonosis Control, Hokkaido University, N20, W10, Kita-ku, Sapporo, 001-0020, Japan.
Akira
A
Kawaguchi
Division of Molecular Pathobiology, Research Center for Zoonosis Control, Hokkaido University, N20, W10, Kita-ku, Sapporo, 001-0020, Japan.
Michihito
M
Sasaki
Division of Bioinformatics, Research Center for Zoonosis Control, Hokkaido University, N20, W10, Kita-ku, Sapporo, 001-0020, Japan.
Gabriel
G
Gonzalez
Division of Bioinformatics, Research Center for Zoonosis Control, Hokkaido University, N20, W10, Kita-ku, Sapporo, 001-0020, Japan.
Kimihito
K
Ito
Hokudai Center for Zoonosis Control in Zambia, Research Center for Zoonosis Control, Hokkaido University, Lusaka, Zambia.
Yuka
Y
Thomas
Department of Para-clinical studies, School of Veterinary Medicine, University of Zambia, Lusaka, Zambia.
Bernard M
BM
Hang'ombe
Department of Disease Control, School of Veterinary Medicine, University of Zambia, Lusaka, Zambia.
Aaron S
AS
Mweene
Department of Pathology and Immunology, Washington University School of Medicine, St. Louis, MO, 63110, USA.
Guoyan
G
Zhao
Molecular Microbiology and Pathology and Immunology, Washington University School of Medicine, St. Louis, MO, 63110, USA.
David
D
Wang
Division of Molecular Pathobiology, Research Center for Zoonosis Control, Hokkaido University, N20, W10, Kita-ku, Sapporo, 001-0020, Japan.
Yasuko
Y
Orba
Hokudai Center for Zoonosis Control in Zambia, Research Center for Zoonosis Control, Hokkaido University, Lusaka, Zambia.
Akihiro
A
Ishii
Global Station for Zoonosis Control, Global Institution for Collaborative Research and Education (GI-CoRE), Hokkaido University, N20, W10, Kita-ku, Sapporo, 001-0020, Japan. h-sawa@czc.hokudai.ac.jp.|Division of Molecular Pathobiology, Research Center for Zoonosis Control, Hokkaido University, N20, W10, Kita-ku, Sapporo, 001-0020, Japan. h-sawa@czc.hokudai.ac.jp.
Hirofumi
H
Sawa
D000818:Animals; D015496:CD4-Positive T-Lymphocytes / Q000821:virology; D016663:Cercopithecinae / Q000821:virology*; D018920:Coculture Techniques; D006801:Humans; D010215:Papio; D010802:Phylogeny; D012367:RNA, Viral / Q000235:genetics*; D060888:Real-Time Polymerase Chain Reaction; D016097:Simian Acquired Immunodeficiency Syndrome / Q000453:epidemiology* / Q000821:virology; D015302:Simian Immunodeficiency Virus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D013154:Spleen / Q000821:virology; D015024:Zambia / Q000453:epidemiology
D012367:RNA, Viral