Quercetin changes purinergic enzyme activities and oxidative profile in platelets of rats with hypothyroidism.
Diseases related to thyroid hormones have been extensively studied because affect a large number of individuals, and these hormones participate in the regulation of the whole organism homeostasis. However, little is known about the involvement of purinergic signaling related to oxidative stress in hypothyroidism and possible therapeutic adjuncts for treatment of this disorder. Thus, the present study investigates the effects of quercetin on NTPDase, 5'-nucleotidase and adenosine deaminase activities, platelet aggregation and oxidative profile in platelets of rats with methimazole (MMI)-induced hypothyroidism. Methimazole at a concentration of 20mg/100mL was administered for 90days. From the second month the animals received quercetin 10 or 25mg/kg for 60days. Results showed that: Ecto-5'-nucleotidase activity decreased in methimazole/water group and the treatment with quercetin 25mg/kg decreased NTPDase, 5'-nucleotidase and adenosine deaminase activities. Moreover, platelet aggregation increased in methimazole/water group. Lipid peroxidation increased while superoxide dismutase and catalase activities decreased, but, interestingly, the treatment with quercetin reversed these changes. These results demonstrated that quercetin modulates adenine nucleotide hydrolysis decreasing the ADP formation and adenosine deamination. At the same time quercetin improves the oxidative profile, as well as reduces platelet aggregation, which together with the modulation in the nucleotides levels can contribute to the prevention of platelet disorders.
Programa de Pós-Graduação em Ciências Biológicas: Bioquímica Toxicológica, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria, Campus Universitário, Camobi, 97105-900 Santa Maria, RS, Brazil. Electronic address: jucimarabaldissarelli@gmail.com.
Jucimara
J
Baldissarelli
Universidade Federal de Mato Grosso, Conselho de Ensino e Pesquisa, Curso de Medicina. Parque Sagrada Família, Rondonópolis, MT, Brazil.
Adriana
A
Santi
Instituto Federal de Educação, Ciência e Tecnologia do Rio Grande do Sul, Campus Bento Gonçalves, RS, Brazil.
Roberta
R
Schmatz
Programa de Pós-Graduação em Ciências Biológicas: Bioquímica Toxicológica, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria, Campus Universitário, Camobi, 97105-900 Santa Maria, RS, Brazil.
Daniela
D
Zanini
Universidade Federal da Fronteira Sul, Campus Chapecó, Chapecó, SC, Brazil.
Andréia M
AM
Cardoso
Universidade Luterana do Brasil-Campus Carazinho, RS, Brazil.
Fátima H
FH
Abadalla
Programa de Pós-Graduação em Ciências Biológicas: Bioquímica Toxicológica, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria, Campus Universitário, Camobi, 97105-900 Santa Maria, RS, Brazil.
Gustavo R
GR
Thomé
Programa de Pós-Graduação em Ciências Biológicas: Bioquímica Toxicológica, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria, Campus Universitário, Camobi, 97105-900 Santa Maria, RS, Brazil.
Camila
C
Murussi
Programa de Pós-Graduação em Ciências Biológicas: Bioquímica Toxicológica, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria, Campus Universitário, Camobi, 97105-900 Santa Maria, RS, Brazil.
Carla R N
CRN
Polachini
Programa de Pós-Graduação em Ciências Biológicas: Bioquímica Toxicológica, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria, Campus Universitário, Camobi, 97105-900 Santa Maria, RS, Brazil.
Diéssica P
DP
Delenogare
Programa de Pós-Graduação em Ciências Biológicas: Bioquímica Toxicológica, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria, Campus Universitário, Camobi, 97105-900 Santa Maria, RS, Brazil.
Vania L
VL
Loro
Programa de Pós-Graduação em Ciências Biológicas: Bioquímica Toxicológica, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria, Campus Universitário, Camobi, 97105-900 Santa Maria, RS, Brazil.
Vera M
VM
Morsch
Programa de Pós-Graduação em Ciências Biológicas: Bioquímica Toxicológica, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria, Campus Universitário, Camobi, 97105-900 Santa Maria, RS, Brazil. Electronic address: mariachitolina@gmail.com.
Maria R C
MRC
Schetinger
D000227:Adenine Nucleotides / Q000097:blood; D000243:Adenosine Deaminase / Q000097:blood*; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D001792:Blood Platelets / Q000187:drug effects* / Q000201:enzymology; D002374:Catalase / Q000097:blood; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D006868:Hydrolysis; D007037:Hypothyroidism / Q000097:blood / Q000139:chemically induced / Q000188:drug therapy* / Q000201:enzymology; D007700:Kinetics; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D008565:Membrane Proteins / Q000097:blood; D008713:Methimazole; D015513:Oncogene Proteins / Q000097:blood*; D018384:Oxidative Stress / Q000187:drug effects*; D010974:Platelet Aggregation / Q000187:drug effects*; D010975:Platelet Aggregation Inhibitors / Q000494:pharmacology*; D011794:Quercetin / Q000494:pharmacology*; D017208:Rats, Wistar; D013482:Superoxide Dismutase / Q000097:blood
D000227:Adenine Nucleotides; D000975:Antioxidants; C485536:Entpd5 protein, rat; D008565:Membrane Proteins; D015513:Oncogene Proteins; D010975:Platelet Aggregation Inhibitors; D008713:Methimazole; D011794:Quercetin; D002374:Catalase; D013482:Superoxide Dismutase; C548484:Ada protein, rat; D000243:Adenosine Deaminase
Ectonucleotidases; Hypothyroidism; Oxidative stress; Platelet aggregation; Quercetin