Cross-Reactive and Cross-Neutralizing Activity of Human Mumps Antibodies Against a Novel Mumps Virus From Bats.
To evaluate the antigenic relationship between bat mumps virus (BMV) and the JL5 vaccine strain of mumps virus (MuVJL5), we rescued a chimeric virus bearing the F and HN glycoproteins of BMV in the background of a recombinant JL5 genome (rMuVJL5). Cross-reactivity and cross-neutralization between this chimeric recombinant MuV bearing the F and HN glycoproteins of BMV (rMuVJL5-F/HNBMV) virus and rMuVJL5 were demonstrated using hyperimmune mouse serum samples and a curated panel of human serum. All mouse and human serum samples that were able to neutralize rMuVJL5 infection had cross-neutralizing activity against rMuVJL5-F/HNBMV. Our data suggest that persons who have neutralizing antibodies against MuV might be protected from infection by BMV.
Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York.
Shannon M
SM
Beaty
Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York.
Raffael
R
Nachbagauer
Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York.
Ariana
A
Hirsh
Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York.
Frederic
F
Vigant
Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York.
James
J
Duehr
Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York.
Kristopher D
KD
Azarm
Division of Structural Biology, Wellcome Trust Centre for Human Genetics, University of Oxford, United Kingdom.
Alice J
AJ
Stelfox
Division of Structural Biology, Wellcome Trust Centre for Human Genetics, University of Oxford, United Kingdom.
Thomas A
TA
Bowden
Department of Microbiology, Boston University School of Medicine, Massachusetts.
W Paul
WP
Duprex
Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York.
Florian
F
Krammer
Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York.
Benhur
B
Lee
D000293:Adolescent; D000328:Adult; D000818:Animals; D057134:Antibodies, Neutralizing / Q000097:blood*; D000914:Antibodies, Viral / Q000097:blood*; D002685:Chiroptera / Q000821:virology*; D003429:Cross Reactions; D005260:Female; D006801:Humans; D008807:Mice, Inbred BALB C; D008875:Middle Aged; D009109:Mumps virus / Q000276:immunology* / Q000302:isolation & purification; D055815:Young Adult
D057134:Antibodies, Neutralizing; D000914:Antibodies, Viral
cross-reactive antibodies; mumps virus; neutralizing antibodies; viral envelope proteins; zoonoses