Chip-based dual-molecularly imprinted monolithic capillary array columns coated Ag/GO for selective extraction and simultaneous determination of bisphenol A and nonyl phenol in fish samples.
An analytical technique for selective, simultaneous determination of bisphenol A (BPA) and nonyl phenol (NP) in fish samples was established by a solid-phase extraction chip integrated with array columns of dual-molecularly imprinted polymer-coated silver-modified graphene oxide (Ag/GO-dual-MISPE-chip). Ag/GO dual-molecularly imprinted polymers (Ag/GO-dual-MIPs) were synthesized by in situ polymerization using Ag/GO as the supporting matrix which extracted templates easily. The affinity and specificity on the Ag/GO-dual-MISPE monolithic column were also investigated. The array extraction chip showed high binding capacity and selectivity to BPA and NP, with the imprinting factors of BPA and NP reaching 2.6 and 2.9, respectively. Ag/GO-dual-MIPs were characterized by scanning electron microscopy and Fourier transform-infrared (FT-IR) spectroscopy. As Low as 2.4ngL-1BPA and 4.7ngL-1NP were detected by high performance liquid chromatography coupled with fluorescence detection. The enrichment factor was 113-fold for BPA and 92-fold for NP. Therefore, the chip-based array columns are feasibly applicable to facile extraction of BPA and NP and effective clean-up of impurities in fish samples.
College of Pharmacy, Guangdong Pharmaceutical University, Guangzhou 510006, China.
Lu
L
Huang
College of Pharmacy, Guangdong Pharmaceutical University, Guangzhou 510006, China. Electronic address: zhaihaiyun@126.com.
Haiyun
H
Zhai
College of Pharmacy, Guangdong Pharmaceutical University, Guangzhou 510006, China.
Guohuan
G
Liang
College of Pharmacy, Guangdong Pharmaceutical University, Guangzhou 510006, China.
Zihao
Z
Su
College of Pharmacy, Guangdong Pharmaceutical University, Guangzhou 510006, China.
Kaisong
K
Yuan
College of Pharmacy, Guangdong Pharmaceutical University, Guangzhou 510006, China.
Guoqiang
G
Lu
College of Pharmacy, Guangdong Pharmaceutical University, Guangzhou 510006, China.
Yufang
Y
Pan
D000818:Animals; D001559:Benzhydryl Compounds / Q000032:analysis*; D053801:Capillary Electrochromatography; D002851:Chromatography, High Pressure Liquid; D005399:Fishes / Q000378:metabolism*; D006108:Graphite / Q000737:chemistry*; D008838:Microcomputers; D008855:Microscopy, Electron, Scanning; D054802:Molecular Imprinting; D010636:Phenols / Q000032:analysis*; D015203:Reproducibility of Results; D012834:Silver / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D017550:Spectroscopy, Fourier Transform Infrared; D000098826:Bisphenol A Compounds
D001559:Benzhydryl Compounds; D006108:Graphite; D010636:Phenols; D012834:Silver; C025256:nonylphenol; C006780:bisphenol A; D000098826:Bisphenol A Compounds
Array column; Dual-molecularly imprinted polymer; Fish; Silver-modified graphene oxide; Solid-phase extraction