Contribution of HN protein length diversity to Newcastle disease virus virulence, replication and biological activities.
To evaluate the contribution of length diversity in the hemagglutinin-neuraminidase (HN) protein to the pathogenicity, replication and biological characteristics of Newcastle disease virus (NDV), we used reverse genetics to generate a series of recombinant NDVs containing truncated or extended HN proteins based on an infectious clone of genotype VII NDV (SG10 strain). The mean death times and intracerebral pathogenicity indices of these viruses showed that the different length mutations in the HN protein did not alter the virulence of NDV. In vitro studies of recombinant NDVs containing truncated or extended HN proteins revealed that the extension of HN protein increased its hemagglutination titer, receptor-binding ability and impaired its neuraminidase activity, fusogenic activity and replication ability. Furthermore, the hemadsorption, neuraminidase and fusogenic promotion activities at the protein level were consistent with those of viral level. Taken together, our results demonstrate that the HN biological activities affected by the C-terminal extension are associated with NDV replication but not the virulence.
Key Laboratory of Animal Epidemiology and Zoonoses, Ministry of Agriculture, College of Veterinary Medicine, China Agricultural University, Beijing 100193, People's Republic of China.
Jihui
J
Jin
Key Laboratory of Animal Epidemiology and Zoonoses, Ministry of Agriculture, College of Veterinary Medicine, China Agricultural University, Beijing 100193, People's Republic of China.
Jing
J
Zhao
Key Laboratory of Animal Epidemiology and Zoonoses, Ministry of Agriculture, College of Veterinary Medicine, China Agricultural University, Beijing 100193, People's Republic of China.
Yingchao
Y
Ren
Key Laboratory of Animal Epidemiology and Zoonoses, Ministry of Agriculture, College of Veterinary Medicine, China Agricultural University, Beijing 100193, People's Republic of China.
Qi
Q
Zhong
Key Laboratory of Animal Epidemiology and Zoonoses, Ministry of Agriculture, College of Veterinary Medicine, China Agricultural University, Beijing 100193, People's Republic of China.
Guozhong
G
Zhang
D000595:Amino Acid Sequence; D000818:Animals; D002642:Chick Embryo; D002522:Chlorocebus aethiops; D018388:Codon, Terminator; D004912:Erythrocytes / Q000821:virology; D015726:Giant Cells / Q000821:virology; D015707:HN Protein / Q000235:genetics* / Q000378:metabolism; D006461:Hemolysis; D020125:Mutation, Missense; D009521:Newcastle Disease / Q000821:virology*; D009522:Newcastle disease virus / Q000201:enzymology* / Q000472:pathogenicity / Q000502:physiology; D014709:Vero Cells; D014774:Virulence; D014779:Virus Replication
D018388:Codon, Terminator; D015707:HN Protein