Depot-specific differences in angiogenic capacity of adipose tissue in differential susceptibility to diet-induced obesity.
Adipose tissue (AT) expansion requires AT remodeling, which depends on AT angiogenesis. Modulation of AT angiogenesis could have therapeutic promise for the treatment of obesity. However, it is unclear how the capacity of angiogenesis in each adipose depot is affected by over-nutrition. Therefore, we investigated the angiogenic capacity (AC) of subcutaneous and visceral fats in lean and obese mice.



We compared the AC of epididymal fat (EF) and inguinal fat (IF) using an angiogenesis assay in diet-induced obese (DIO) mice and diet-resistant (DR) mice fed a high-fat diet (HFD). Furthermore, we compared the expression levels of genes related to angiogenesis, macrophage recruitment, and inflammation using RT-qPCR in the EF and IF of lean mice fed a low-fat diet (LFD), DIO mice, and DR mice fed a HFD.



DIO mice showed a significant increase in the AC of EF only at 22 weeks of age compared to DR mice. The expression levels of genes related to angiogenesis, macrophage recruitment, and inflammation were significantly higher in the EF of DIO mice than in those of LFD mice and DR mice, while expression levels of genes related to macrophages and their recruitment were higher in the IF of DIO mice than in those of LFD and DR mice. Expression of genes related to angiogenesis (includingHif1a,Vegfa,Fgf1,Kdr, andPecam1), macrophage recruitment, and inflammation (includingEmr1,Ccr2,Itgax,Ccl2,Tnf, andIl1b) correlated more strongly with body weight in the EF of HFD-fed obese mice compared to that of IF.



These results suggest depot-specific differences in AT angiogenesis and a potential role in the susceptibility to diet-induced obesity.
Department of Pharmacology, Korea University College of Medicine, Seoul, Republic of Korea.
Mun-Gyu
MG
Song
Department of Pharmacology, Korea University College of Medicine, Seoul, Republic of Korea.
Hye-Jin
HJ
Lee
Department of Pharmacology, Korea University College of Medicine, Seoul, Republic of Korea.
Bo-Yeong
BY
Jin
División de Investigación, Facultad de Medicina, Universidad Nacional Autónoma de México (UNAM), Mexico City, Mexico; Laboratorio de Enfermedades Metabólicas: Obesidad y Diabetes, Hospital Infantil de México "Federico Gómez", Mexico City, Mexico.
Ruth
R
Gutierrez-Aguilar
Department of Pharmacology, Korea University College of Medicine, Seoul, Republic of Korea.
Kyung-Ho
KH
Shin
Department of Pharmacology, Korea University College of Medicine, Seoul, Republic of Korea.
Sang-Hyun
SH
Choi
Department of Molecular Cell Biology, Samsung Biomedical Research Institute, Sungkyunkwan University School of Medicine, Suwon, Republic of Korea. Electronic address: shum@skku.edu.
Sung Hee
SH
Um
Department of Pharmacology, Korea University College of Medicine, Seoul, Republic of Korea. Electronic address: LDHKIM@korea.ac.kr.
Dong-Hoon
DH
Kim
D000273:Adipose Tissue / Q000378:metabolism*; D000818:Animals; D059305:Diet, High-Fat; D015870:Gene Expression; D051379:Mice; D008810:Mice, Inbred C57BL; D008820:Mice, Obese; D018919:Neovascularization, Physiologic / Q000235:genetics / Q000502:physiology*; D009765:Obesity / Q000378:metabolism*
Adipose tissue; Angiogenesis; Diet-induced obese mice; Diet-resistant mice; High-fat diet; Inflammation