Patterning of Endothelial Cells and Mesenchymal Stem Cells by Laser-Assisted Bioprinting to Study Cell Migration.
Tissue engineering of large organs is currently limited by the lack of potent vascularizationin vitro. Tissue-engineered bone grafts can be prevascularizedin vitrousing endothelial cells (ECs). The microvascular network architecture could be controlled by printing ECs following a specific pattern. Using laser-assisted bioprinting, we investigated the effect of distance between printed cell islets and the influence of coprinted mesenchymal cells on migration. When printed alone, ECs spread out evenly on the collagen hydrogel, regardless of the distance between cell islets. However, when printed in coculture with mesenchymal cells by laser-assisted bioprinting, they remained in the printed area. Therefore, the presence of mesenchymal cell is mandatory in order to create a pattern that will be conserved over time. This work describes an interesting approach to study cell migration that could be reproduced to study the effect of trophic factors.
Bioingénierie Tissulaire, INSERM U1026, Bordeaux, France; Département d'Ophtalmologie, Université de Montréal, Montréal, QC, Canada.
Jean-Michel
0000-0003-1462-7392
JM
Bourget
Bioingénierie Tissulaire, INSERM U1026, Bordeaux, France; Bioingénierie Tissulaire, Université de Bordeaux, Bordeaux, France; Service d'Odontologie et de Santé Buccale, CHU de Bordeaux, Bordeaux, France.
Olivia
0000-0001-7967-5569
O
Kérourédan
Bioingénierie Tissulaire, INSERM U1026, Bordeaux, France; Bioingénierie Tissulaire, Université de Bordeaux, Bordeaux, France.
Manuela
M
Medina
Bioingénierie Tissulaire, INSERM U1026, Bordeaux, France; Bioingénierie Tissulaire, Université de Bordeaux, Bordeaux, France.
Murielle
M
Rémy
Bioingénierie Tissulaire, INSERM U1026, Bordeaux, France; Bioingénierie Tissulaire, Université de Bordeaux, Bordeaux, France; Service d'Odontologie et de Santé Buccale, CHU de Bordeaux, Bordeaux, France.
Noélie Brunehilde
NB
Thébaud
Bioingénierie Tissulaire, INSERM U1026, Bordeaux, France.
Reine
R
Bareille
Bioingénierie Tissulaire, INSERM U1026, Bordeaux, France; Bioingénierie Tissulaire, Université de Bordeaux, Bordeaux, France.
Olivier
O
Chassande
Bioingénierie Tissulaire, INSERM U1026, Bordeaux, France; Bioingénierie Tissulaire, Université de Bordeaux, Bordeaux, France.
Joëlle
J
Amédée
Bioingénierie Tissulaire, INSERM U1026, Bordeaux, France; Bioingénierie Tissulaire, Université de Bordeaux, Bordeaux, France.
Sylvain
S
Catros
Bioingénierie Tissulaire, INSERM U1026, Bordeaux, France; Bioingénierie Tissulaire, Université de Bordeaux, Bordeaux, France; Service d'Odontologie et de Santé Buccale, CHU de Bordeaux, Bordeaux, France.
Raphaël
0000-0002-2962-2044
R
Devillard
D062028:Bioprinting / Q000379:methods*; D002465:Cell Movement; D018920:Coculture Techniques; D005260:Female; D061307:Human Umbilical Vein Endothelial Cells / Q000166:cytology / Q000378:metabolism*; D006801:Humans; D007834:Lasers; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism*