Syntenin-1 is a promoter and prognostic marker of head and neck squamous cell carcinoma invasion and metastasis.
Metastasis represents a key factor associated with poor prognosis of head and neck squamous cell carcinoma (HNSC). However, the underlying molecular mechanisms remain largely unknown. In this study, our liquid chromatography with tandem mass spectrometry analysis revealed a number of significantly differentially expressed membrane/membrane-associated proteins between high invasive UM1 and low invasive UM2 cells. One of the identified membrane proteins, Syntenin-1, was remarkably up-regulated in HNSC tissues and cell lines when compared to the controls, and also over-expressed in recurrent HNSC and high invasive UM1 cells. Syntenin-1 over-expression was found to be significantly associated with lymph node metastasis and disease recurrence. HNSC patients with higher syntenin-1 expression had significantly poorer long term overall survival and similar results were found in many other types of cancers based on analysis of The Cancer Genome Atlas data. Finally, knockdown of syntenin-1 inhibited the proliferation, migration and invasion of HNSC cells, and opposite findings were observed when syntenin-1 was over-expressed. Collectively, our studies indicate that syntenin-1 promotes invasion and progression of HNSC. It may serve as a valuable biomarker for lymph node metastasis or a potential target for therapeutic intervention in HNSC.
University of California at Los Angeles, School of Dentistry, Los Angeles, CA 90095, USA.|University of California at Los Angeles, Jonsson Comprehensive Cancer Center, Los Angeles, CA 90095, USA.
Li
L
Cui
University of California at Los Angeles, School of Dentistry, Los Angeles, CA 90095, USA.|University of California at Los Angeles, Department of Statistics, Los Angeles, CA 90095, USA.
Siliangyu
S
Cheng
University of California at Los Angeles, School of Dentistry, Los Angeles, CA 90095, USA.
Xiaojun
X
Liu
University of California at Los Angeles, School of Dentistry, Los Angeles, CA 90095, USA.|University of California at Los Angeles, Jonsson Comprehensive Cancer Center, Los Angeles, CA 90095, USA.
Diana
D
Messadi
University of California at Los Angeles, School of Dentistry, Los Angeles, CA 90095, USA.|Department of Stomatology, Zhongnan Hospital, Wuhan University, Wuhan 430071, China.
Yan
Y
Yang
University of California at Los Angeles, School of Dentistry, Los Angeles, CA 90095, USA.|University of California at Los Angeles, Jonsson Comprehensive Cancer Center, Los Angeles, CA 90095, USA.
Shen
S
Hu
D000368:Aged; D014408:Biomarkers, Tumor / Q000235:genetics / Q000378:metabolism*; D002294:Carcinoma, Squamous Cell / Q000235:genetics / Q000378:metabolism* / Q000556:secondary / Q000628:therapy; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D002853:Chromatography, Liquid; D005260:Female; D006258:Head and Neck Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology / Q000628:therapy; D006801:Humans; D053208:Kaplan-Meier Estimate; D008207:Lymphatic Metastasis; D008297:Male; D008875:Middle Aged; D009361:Neoplasm Invasiveness; D009364:Neoplasm Recurrence, Local; D040901:Proteomics / Q000379:methods; D034622:RNA Interference; D015398:Signal Transduction; D000077195:Squamous Cell Carcinoma of Head and Neck; D053676:Syntenins / Q000235:genetics / Q000378:metabolism*; D053719:Tandem Mass Spectrometry; D013997:Time Factors; D014162:Transfection
D014408:Biomarkers, Tumor; C109621:SDCBP protein, human; D053676:Syntenins
head and neck squamous cell carcinoma; membrane proteins; metastasis; syntenin-1