Multiplex immunoassays in endometriosis: An array of possibilities.
Multiplex immunoassays range from small-scaled multiplex sandwich ELISAs in a planar or bead-based format to the more expanded antibody arrays employing direct sample labeling. The plethora of data generated from these arrays could be of great interest to understand a complex disorder such as endometriosis. Multiplex immunoassay analysis may provide information on disease pathology and may lead to improved, timely diagnosis. Until now, the use of multiplex immunoassays has been limited in endometriosis. With the constant development of multiplex technologies, future studies should focus on implementing these techniques, and combining them with multivariate statistical analysis. In this review, we provide an overview of multiplex immunoassay methods used in endometriosis studies and the data sets acquired by these methodologies. These data and future studies might provide novel insights for biomarker discovery and investigation of the pathogenesis in endometriosis.
KU Leuven, Department of Development and Regeneration, Organ systems, Leuven, Belgium and Department of Obstetrics and Gynaecology, Leuven University Fertility Centre, University Hospital Leuven, Leuven, Belgium.
Dorien F
DF
O
Department of Electrical Engineering (ESAT), STADIUS Center for Dynamical Systems, Signal Processing, and Data Analytics, KU Leuven, Kasteelpark Arenberg 10, Box 2446, 3001 Leuven, Belgium.
Yousef
Y
El Aalamat
KU Leuven, Department of Cellular and Molecular Medicine, Campus Gasthuisberg, Leuven, Belgium and SyBioMa, Interfaculty Centre for Systems Biology based Mass Spectrometry, Campus Gasthuisberg, Leuven, Belgium.
Etienne
E
Waelkens
Department of Electrical Engineering (ESAT), STADIUS Center for Dynamical Systems, Signal Processing, and Data Analytics, KU Leuven, Kasteelpark Arenberg 10, Box 2446, 3001 Leuven, Belgium.
Bart
B
De Moor
KU Leuven, Department of Development and Regeneration, Organ systems, Leuven, Belgium and Department of Obstetrics and Gynaecology, Leuven University Fertility Centre, University Hospital Leuven, Leuven, Belgium.
Thomas
T
D'Hooghe
KU Leuven, Department of Development and Regeneration, Organ Systems, Leuven, Belgium, 2Department of Obstetrics and Gynaecology, Leuven University Fertility Centre, University Hospital Leuven, Leuven, Belgium, amelie.fassbender@yahoo.com.
Amelie
A
Fassbender
D001202:Ascitic Fluid / Q000378:metabolism; D001798:Blood Proteins / Q000378:metabolism; D004715:Endometriosis / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005260:Female; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D015999:Multivariate Analysis; D011506:Proteins / Q000378:metabolism
D001798:Blood Proteins; D011506:Proteins