Association study between mannose-binding lectin haplotypes and X gene mutation of hepatitis B virus from treatment naïve patients.
Mannose binding lectin (MBL) plays important role in the innate immunity of human. Mutations in the MBL2 gene can significantly change the serum level of MBL, and consequently alter the susceptibility and progression of infectious disease. However, the association between the MBL2 profile and the HBV mutation and quasispecies complexity has not yet been reported. Our approach includes the study of the MBL2 gene genotype as well as ultra-deep sequencing of the HBV viruses obtained from the plasma of 50 treatment naïve patients with chronic HBV infection. We found that the liver function was better among patients within the high MBL2 group with respect to those within the medium/low MBL2 group. Likewise, the number of mutations in the HBV X gene as well as the viral quasispecies complexity were significantly higher in medium/low MBL2 production group. Nucleotide substitution rates were also higher within the medium/low MBL2 production group in all positions described to have an influence in liver cancer development, except for A1499G. In this work we show that the MBL2 profile may have an impact on the HBV X gene mutations as well as on viral quasispecies complexity.
Xiamen Center for Disease Control and Prevention, Xiamen, Fujian 361021, China.
Chenghao
C
Su
Xiamen Center for Disease Control and Prevention, Xiamen, Fujian 361021, China.|School of Public Health, Fujian Medical University, Fuzhou, Fujian 351022, China.
Yong
Y
Lin
Xiamen Hospital of Traditional Chinses Medicine, Xiamen, Fujian 361001, China.
Qianguo
Q
Mao
Roche Pharma Research and Early Development, Immunology, Inflammation and Infectious Diseases, Roche Innovation Center Shanghai 201203, China.
Daitze
D
Wu
Roche Pharma Research and Early Development, Immunology, Inflammation and Infectious Diseases, Roche Innovation Center Shanghai 201203, China.
Lina
L
Zhu
Roche Pharma Research and Early Development, Immunology, Inflammation and Infectious Diseases, Roche Innovation Center Basel 4070, Switzerland.
Isabel
I
Najera
Roche Pharma Research and Early Development, Immunology, Inflammation and Infectious Diseases, Roche Innovation Center Basel 4070, Switzerland.
Fernando
F
Garcia-Alcalde
Zhongshan Hospital, Xiamen University, Xiamen, Fujian 361004, China.
Jianjun
J
Niu
D000328:Adult; D000368:Aged; D018450:Disease Progression; D005260:Female; D005814:Genes, Viral; D020022:Genetic Predisposition to Disease; D006239:Haplotypes; D006509:Hepatitis B / Q000235:genetics / Q000276:immunology*; D006515:Hepatitis B virus / Q000235:genetics*; D006801:Humans; D007113:Immunity, Innate; D008297:Male; D037601:Mannose-Binding Lectin / Q000097:blood / Q000235:genetics*; D008875:Middle Aged; D009154:Mutation; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods; D055815:Young Adult
D037601:Mannose-Binding Lectin
hepatitis B virus; mannose-binding lectin; mutation; quasispecies complexity; treatment naïve