Extracellular matrix gene expression profiling using microfluidics for colorectal carcinoma stratification.
In cancer, biomarkers have many potential applications including generation of a differential diagnosis, prediction of response to treatment, and monitoring disease progression. Many molecular biomarkers have been put forward for different diseases but most of them do not possess the required specificity and sensitivity. A biomarker with a high sensitivity has a low specificity and vice versa. The inaccuracy of the biomarkers currently in use has led to a compelling need to identify more accurate markers with diagnostic and prognostic significance. The aim of the present study was to use a novel, droplet-based, microfluidic platform to evaluate the prognostic value of a panel of thirty-four genes that regulate the composition of extracellular matrices in colorectal carcinoma. Our method is a novel approach as it uses using continuous-flowing Polymerase Chain Reaction for the sensitive detection and accurate quantitation of gene expression. We identified a panel of relevant extracellular matrix genes whose expression levels were measured by real-time quantitative polymerase chain reaction using Taqman®reagents in twenty-four pairs of matched colorectal cancer tumour and associated normal tissue. Differential expression patterns occurred between the normal and malignant tissue and correlated with histopathological parameters and overall surgical staging. The findings demonstrate that a droplet-based microfluidic quantitative PCR system enables biomarker classification. It was further possible to sub-classify colorectal cancer based on extracellular matrix protein expressing groups which in turn correlated with prognosis.
Christopher J
CJ
Hayes
Catriona M
CM
Dowling
Stokes Laboratories, Bernal Institute, University of Limerick , Limerick, Ireland.
Susan
S
Dwane
Mary E
ME
McCumiskey
Department of Surgery, University Hospital Limerick , Limerick, Ireland.
Shona M
SM
Tormey
Department of Surgery, University Hospital Limerick , Limerick, Ireland.
B
B
Anne Merrigan
John C
JC
Coffey
Patrick A
PA
Kiely
Stokes Laboratories, Bernal Institute, University of Limerick , Limerick, Ireland.
Tara M
TM
Dalton