Identification of G-quadruplexes in long functional RNAs using 7-deazaguanine RNA.
RNA G-quadruplex (G4) structures are thought to affect biological processes, including translation and pre-mRNA splicing, but it is not possible at present to demonstrate that they form naturally at specific sequences in long functional RNA molecules. We developed a new strategy, footprinting of long 7-deazaguanine-substituted RNAs (FOLDeR), that allows the formation of G4s to be confirmed in long RNAs and under functional conditions.
Leicester Institute of Structural and Chemical Biology and Department of Molecular and Cell Biology, University of Leicester, Leicester, UK.
Carika
C
Weldon
IMoPA (Ingénierie Moléculaire et Physiopathologie Articulaire), UMR 7365 Centre National de la Recherche Scientifique (CNRS)-UL, Biopôle de l'Université de Lorraine, Vandoeuvre-lès-Nancy, France.
Isabelle
I
Behm-Ansmant
College of Pharmacy and BIO5 Institute, University of Arizona, Tucson, Arizona, USA.|University of Arizona Cancer Center, Tucson, Arizona, USA.
Laurence H
LH
Hurley
Department of Pure and Applied Chemistry, University of Strathclyde, Glasgow, UK.
Glenn A
GA
Burley
IMoPA (Ingénierie Moléculaire et Physiopathologie Articulaire), UMR 7365 Centre National de la Recherche Scientifique (CNRS)-UL, Biopôle de l'Université de Lorraine, Vandoeuvre-lès-Nancy, France.
Christiane
C
Branlant
Leicester Institute of Structural and Chemical Biology and Department of Molecular and Cell Biology, University of Leicester, Leicester, UK.
Ian C
IC
Eperon
Leicester Institute of Structural and Chemical Biology and Department of Molecular and Cell Biology, University of Leicester, Leicester, UK.
Cyril
http://orcid.org/0000-0003-3575-8922
C
Dominguez
D054856:G-Quadruplexes; D006147:Guanine / Q000031:analogs & derivatives* / Q000737:chemistry / Q000378:metabolism; D006801:Humans; D012313:RNA / Q000737:chemistry* / Q000378:metabolism
D006147:Guanine; D012313:RNA; C066856:7-deazaguanine