MicroRNA Expression Patterns of CD8+ T Cells in Acute and Chronic Brucellosis.
Although our knowledge about Brucella virulence factors and the host response increase rapidly, the mechanisms of immune evasion by the pathogen and causes of chronic disease are still unknown. Here, we aimed to investigate the immunological factors which belong to CD8+ T cells and their roles in the transition of brucellosis from acute to chronic infection. Using miRNA microarray, more than 2000 miRNAs were screened in CD8+ T cells of patients with acute or chronic brucellosis and healthy controls that were sorted from peripheral blood with flow cytometry and validated through qRT-PCR. Findings were evaluated using GeneSpring GX (Agilent) 13.0 software and KEGG pathway analysis. Expression of two miRNAs were determined to display a significant fold change in chronic group when compared with acute or control groups. Both miRNAs (miR-126-5p and miR-4753-3p) were decreased (p <0.05 or fold change > 2). These miRNAs have the potential to be the regulators of CD8+ T cell-related marker genes for chronic brucellosis infections. The differentially expressed miRNAs and their predicted target genes are involved in MAPK signaling pathway, cytokine-cytokine receptor interactions, endocytosis, regulation of actin cytoskeleton, and focal adhesion indicating their potential roles in chronic brucellosis and its progression. It is the first study of miRNA expression analysis of human CD8+ T cells to clarify the mechanism of inveteracy in brucellosis.
Department of Immunology, Faculty of Medicine, Uludag University, Bursa, Turkey.
Ferah
F
Budak
Department of Immunology, Faculty of Medicine, Uludag University, Bursa, Turkey.
S Haldun
SH
Bal
Department of Medical Biology, Faculty of Medicine, Uludag University, Bursa, Turkey.
Gulcin
G
Tezcan
Department of Molecular Genetics, Faculty of Medicine, University of Toronto, Toronto, Canada.
Furkan
F
Guvenc
Department of Clinical Microbiology and Infection Diseases, Faculty of Medicine, Uludag University, Bursa, Turkey.
E Halis
EH
Akalin
Department of Medical Microbiology, Faculty of Medicine, Uludag University, Bursa, Turkey.
Guher
G
Goral
Department of Immunology, Aziz Sancar Institute of Experimental Medicine, Istanbul University, Istanbul, Turkey.
Gunnur
G
Deniz
Department of Immunology, Faculty of Medicine, Uludag University, Bursa, Turkey.
H Barbaros
http://orcid.org/0000-0003-0463-6818
HB
Oral
D000208:Acute Disease; D000328:Adult; D002006:Brucellosis / Q000378:metabolism*; D018414:CD8-Positive T-Lymphocytes / Q000378:metabolism*; D002908:Chronic Disease; D018450:Disease Progression; D005260:Female; D020869:Gene Expression Profiling / Q000379:methods; D054884:Host-Pathogen Interactions / Q000502:physiology; D006801:Humans; D057131:Immune Evasion / Q000502:physiology; D008297:Male; D035683:MicroRNAs / Q000378:metabolism*; D008875:Middle Aged; D015398:Signal Transduction / Q000502:physiology
D035683:MicroRNAs