A Micropatterned Human Pluripotent Stem Cell-Based Ventricular Cardiac Anisotropic Sheet for Visualizing Drug-Induced Arrhythmogenicity.
A novel cardiomimetic biohybrid material, termed as the human ventricular cardiac anisotropic sheet (hvCAS) is reported. Well-characterized human pluripotent stem-cell-derived ventricular cardiomyocytes are strategically aligned to reproduce key electrophysiological features of native human ventricle, which, along with specific selection criteria, allows for a direct visualization of arrhythmic spiral re-entry and represents a revolutionary tool to assess preclinical drug-induced arrhythmogenicity.
Stem Cell & Regenerative Medicine Consortium, LKS Faculty of Medicine, University of Hong Kong, Pokfulam, Hong Kong.
Angie M Y
AM
Shum
Stem Cell & Regenerative Medicine Consortium, LKS Faculty of Medicine, University of Hong Kong, Pokfulam, Hong Kong.
Hui
H
Che
Stem Cell & Regenerative Medicine Consortium, LKS Faculty of Medicine, University of Hong Kong, Pokfulam, Hong Kong.
Andy On-Tik
AO
Wong
Stem Cell & Regenerative Medicine Consortium, LKS Faculty of Medicine, University of Hong Kong, Pokfulam, Hong Kong.
Chenzi
C
Zhang
Biomedical Engineering, Hong Kong University of Science & Technology, Clear Water Bay, Hong Kong.
Hongkai
H
Wu
Novoheart Ltd., Hong Kong Science Park, Shatin, Hong Kong.
Camie W Y
CW
Chan
Icahn School of Medicine at Mount Sinai, Manhattan, NYC, 10029-5674, USA.
Kevin
K
Costa
Biomedical Engineering, University of California at Irvine, Irvine, CA, 92697-2715, USA.
Michelle
M
Khine
Stem Cell & Regenerative Medicine Consortium, LKS Faculty of Medicine, University of Hong Kong, Pokfulam, Hong Kong.
Chi-Wing
CW
Kong
Dr. Li Dak-Sum Research Centre, University of Hong Kong, Pokfulam, Hong Kong.|Ming-Wai Lau Centre for Reparative Medicine, Karolinska Institutet, Stockholm, 17177, Sweden.
Ronald A
RA
Li
D002454:Cell Differentiation; D006352:Heart Ventricles; D006801:Humans; D032383:Myocytes, Cardiac; D039904:Pluripotent Stem Cells
anisotropy; cell alignments; drug-induced arrhythmias; human pluripotent stem cell-derived ventricular cardiomyocytes; micropatterned monolayers