Aqueous enzymatic process for cell wall degradation and lipid extraction from Nannochloropsis sp.
An effective cell disruption method, including alkaline pretreatment and subsequent enzymatic treatment, was established to break cell walls and extract lipid from Nannochloropsis sp. A synergistic effect was found between alkaline pretreatment and enzymatic treatment. The combination of commercialize enzymes (cellulase, protease, lysozyme, and pectinase) achieved higher lipid yield compared with a single enzyme application. With the compromise between economic feasibility and lipid yield, the optimum reaction conditions were obtained with alkaline pretreatment at pH 10.5 at 110°C for 4h, and subsequent enzymatic treatment at pH 4 at 50°C for 30min with the dosage of each enzyme at 200IU/g. As high as 90.0% of lipid was extracted under optimal conditions from Nannochloropsis sp.
Department of Chemical and Petroleum Engineering, University of Calgary, T2N 1N4 Calgary, Alberta, Canada.
Chongchong
C
Wu
Department of Chemical and Petroleum Engineering, University of Calgary, T2N 1N4 Calgary, Alberta, Canada.
Ye
Y
Xiao
Research Institute of Petroleum Processing, Sinopec, Beijing 100083, China.
Weiguo
W
Lin
Research Institute of Petroleum Processing, Sinopec, Beijing 100083, China.
Jiaquan
J
Li
Research Institute of Petroleum Processing, Sinopec, Beijing 100083, China.
Saisai
S
Zhang
Research Institute of Petroleum Processing, Sinopec, Beijing 100083, China.
Junying
J
Zhu
Research Institute of Petroleum Processing, Sinopec, Beijing 100083, China. Electronic address: rongjf.ripp@sinopec.com.
Junfeng
J
Rong
D002473:Cell Wall / Q000737:chemistry* / Q000378:metabolism*; D002480:Cellulase / Q000378:metabolism; D005591:Chemical Fractionation / Q000379:methods; D050356:Lipid Metabolism; D008055:Lipids / Q000302:isolation & purification*; D058086:Microalgae / Q000201:enzymology* / Q000378:metabolism*; D009113:Muramidase / Q000378:metabolism; D011096:Polygalacturonase / Q000378:metabolism; D014867:Water / Q000737:chemistry
D008055:Lipids; D014867:Water; D011096:Polygalacturonase; D009113:Muramidase; D002480:Cellulase
Algae; Alkaline; Degradation; Enzyme; Lipid