Anticancer, antioxidant potential and profiling of polyphenolic compounds ofWrightia tinctoriaRoxb. (R.Br.) bark.
Wrightia tinctoriaRoxb. (R.Br.) is an Ayurvedic remedy, ethnomedically used in the treatment of various ailments. The present work was carried out to evaluate the anticancer and antioxidant activity as well as total phenolic and phytochemical contents ofW. tinctoriabark methanolic extract (WTBM) by high-performance liquid chromatography (HPLC)-diode array detector. Antiproliferative activity of WTBM was evaluated against MDA-MB-231 and MCF-7 cancer cells by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, colony formation, and Hoechst staining. In addition, the antioxidant potential was determined by 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity and 2,2- azino-bis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS) radical cation decolorization assay. Total phenolic content was assessed by Folin-Ciocalteu method. The results demonstrated that WTBM exhibited significant antiproliferative effect against MDA-MB-231 (IC50= 88.9 ± 1.27 μg/ml) and MCF-7 (IC50= 45.71 ± 7.74 μg/ml) cancer cells in time- and dose-dependent manner. WTBM significantly suppresses colony formation and induces apoptosis in both MDA-MB-231 and MCF-7 cells as evident by morphological assessment, clonogenic assay, and Hoechst staining. The total phenolic content of WTBM was found to be 30.3 gallic acid equivalent mg/g dry weight of bark extract while IC50value for DPPH and ABTS radical scavenging activity was 72.2 ± 2.8 μg/ml and 45.16 ± 1.95 μg/ml, respectively. HPLC analysis showed the presence of gallic acid, rutin, and quercetin in WTBM. These findings demonstrated that WTBM significantly inhibited proliferation of breast cancer cells and induced apoptosis, suggesting the potential chemopreventive activity ofW. tinctoriabark.
Department of Biochemistry, Natural Products Research Laboratory, King George's Medical University, Lucknow, Uttar Pradesh, India; Department of Chemistry, Integral University, Lucknow, Uttar Pradesh, India.
Nishat
N
Fatima
Department of Biochemistry, Natural Products Research Laboratory, King George's Medical University, Lucknow, Uttar Pradesh, India.
Mohammad Kaleem
MK
Ahmad
Department of Biochemistry, Natural Products Research Laboratory, King George's Medical University, Lucknow, Uttar Pradesh, India; Department of Chemistry, Integral University, Lucknow, Uttar Pradesh, India.
Jamal Akhtar
JA
Ansari
Department of Chemistry, Integral University, Lucknow, Uttar Pradesh, India.
Zulfiqar
Z
Ali
Department of Chemistry, Integral University, Lucknow, Uttar Pradesh, India.
Abdul Rahman
AR
Khan
Department of Biochemistry, Natural Products Research Laboratory, King George's Medical University, Lucknow, Uttar Pradesh, India.
Abbas Ali
AA
Mahdi
Antioxidant; Wrightia tinctoria; antiproliferative; breast cancer; high-performance liquid chromatography