Electrochemical luminescence determination of hyperin using a sol-gel@graphene luminescent composite film modified electrode for solid phase microextraction.
In this paper, a novel electrochemiluminescence (ECL) sensor of sol-gel@graphene luminescent composite film modified electrode for hyperin determination was prepared using graphene (G) as solid-phase microextraction (SPME) material, based on selective preconcentration of target onto an electrode and followed by luminol ECL detection. Hyperin was firstly extracted from aqueous solution through the modified GCE. Hydrogel, electrogenerated chemiluminescence reagents, pH of working solution, extraction time and temperature and scan rate were discussed. Under the optimum conditions, the change of ECL intensity was in proportion to the concentration of hyperin in the range of 0.02-0.24μg/mL with a detection limit of 0.01μg/mL. This method showed good performance in stability, reproducibility and precision for the determination of hyperin.
Shandong Shengli Bioengineering Co., LTD., Jining 272000, Shandong, China.
Xiaojun
X
Zou
Faculty of Materials Science and Chemical Engineering, State Key Laboratory Base of Novel Functional Materials and Preparation Science, Ningbo University, Ningbo 315211, China.
Fang
F
Shang
Faculty of Materials Science and Chemical Engineering, State Key Laboratory Base of Novel Functional Materials and Preparation Science, Ningbo University, Ningbo 315211, China. Electronic address: wjydjy2011@163.com.
Sui
S
Wang
Electrochemiluminescence; Hyperin; Modified electrode; Sol-gel@graphene luminescent composite film