Alginate Encapsulation of Human Hepatocytes and Assessment of Microbeads.
Alginate encapsulation of cells is an attractive technique in which alginate becomes polymerized entrapping the cells. The structure of formed microbeads/microcapsules is semipermeable as it allows oxygen and nutrients to go in, and waste products and other materials produced by the cells to go out. Here, we describe basic protocols for alginate encapsulation of human hepatocytes and methods for assessing the microbeads produced.
Institute of Liver Studies, King's College Hospital, Bessemer Road, London, SE5 9PJ, UK. ragai.mitry@kcl.ac.uk.
Ragai R
RR
Mitry
Institute of Liver Studies, King's College Hospital, Bessemer Road, London, SE5 9PJ, UK.
Suttiruk
S
Jitraruch
Institute of Liver Studies, King's College Hospital, Bessemer Road, London, SE5 9PJ, UK.
Valeria
V
Iansante
Institute of Liver Studies, King's College Hospital, Bessemer Road, London, SE5 9PJ, UK.
Anil
A
Dhawan
D000464:Alginates / Q000737:chemistry*; D002214:Capsules / Q000737:chemistry; D018929:Cell Culture Techniques; D002469:Cell Separation / Q000379:methods; D002470:Cell Survival; D017690:Cell Transplantation / Q000295:instrumentation / Q000379:methods*; D003124:Colorimetry / Q000379:methods; D004339:Drug Compounding / Q000295:instrumentation / Q000379:methods*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D020723:Glucuronic Acid / Q000737:chemistry; D022781:Hepatocytes / Q000502:physiology / Q000637:transplantation*; D006603:Hexuronic Acids / Q000737:chemistry; D006801:Humans; D008099:Liver / Q000166:cytology / Q000601:surgery; D008853:Microscopy; D008863:Microspheres
D000464:Alginates; D002214:Capsules; D006603:Hexuronic Acids; D020723:Glucuronic Acid
Alginate; Encapsulation; Hepatocyte; Microbeads; Microcapsules