Neuroprotective Effect of Melatonin Against PCBs Induced Behavioural, Molecular and Histological Changes in Cerebral Cortex of Adult Male Wistar Rats.
There is ample evidence stating Polychlorinated biphenyls (PCBs) as neurotoxins. In the current study, we have analyzed the behavioural impact of PCBs exposure in adult rats and assessed the simultaneous effect of antioxidant melatonin against the PCBs action. The rats were grouped into four and treated intraperitoneally with vehicle, PCBs, PCBs + melatonin and melatonin alone for 30 days, respectively. After the treatment period the rats were tested for locomotor activity and anxiety behaviour analysis. We confirmed the neuronal damage in the cerebral cortex by molecular and histological analysis. Our data indicates that there is impairment in locomotor activity and behaviour of PCBs treated rats compared to control. The simultaneous melatonin treated rat shows increased motor coordination and less anxiety like behaviour compared to PCBs treated rats. Molecular and histological analysis supports that, the impaired motor coordination in PCBs treated rats is due to neurodegeneration in motor cortex region. The results proved that melatonin treatment improved the motor co-ordination and reduced anxiety behaviour, prevented neurodegeneration in the cerebral cortex of PCBs-exposed adult male rats.
Department of Endocrinology, Dr. ALM Post Graduate Institute of Basic Medical Sciences, University of Madras, Chennai, 600 113, India.
S
S
Bavithra
Department of Clinical Laboratory, Kauvery Hospital and Billroth Hospitals, Chennai, India.
K
K
Selvakumar
Department of Physiology, Dr. ALM Post Graduate Institute of Basic Medical Sciences, University of Madras, Chennai, 600 113, India.
L
L
Sundareswaran
Department of Endocrinology, Dr. ALM Post Graduate Institute of Basic Medical Sciences, University of Madras, Chennai, 600 113, India. j_arunakaran@hotmail.com.
J
J
Arunakaran
D000818:Animals; D000071187:Autophagy-Related Protein 5 / Q000378:metabolism; D002540:Cerebral Cortex / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology*; D004785:Environmental Pollutants / Q000633:toxicity; D008124:Locomotion / Q000187:drug effects / Q000502:physiology*; D008297:Male; D018782:Maze Learning / Q000187:drug effects / Q000502:physiology; D008550:Melatonin / Q000494:pharmacology*; D018696:Neuroprotective Agents / Q000494:pharmacology*; D018384:Oxidative Stress / Q000187:drug effects / Q000502:physiology; D011078:Polychlorinated Biphenyls / Q000633:toxicity*; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar
D000071187:Autophagy-Related Protein 5; D004785:Environmental Pollutants; D018696:Neuroprotective Agents; D011078:Polychlorinated Biphenyls; D008550:Melatonin
Anxiety; Apoptosis; Melatonin; Neurodegeneration; PCBs; Rats