Pine Defensive Monoterpene α-Pinene Influences the Feeding Behavior of Dendroctonus valens and Its Gut Bacterial Community Structure.
The exposure to plant defense chemicals has negative effects on insect feeding activity and modifies insect gut microbial community composition.Dendroctonus valensis a very destructive forest pest in China, and harbors a large diversity and abundance of gut microorganisms. Host pine defensive chemicals can protect the pines from attack by the holobiont. In this study, boring length ofD. valensfeeding on 0 mg/g α-pinene and 9 mg/g α-pinene concentration in phloem media for 6 and 48 h were recorded, and their gut bacterial communities were analyzed in parallel. Nine milligram per gram α-pinene concentration significantly inhibited boring length ofD. valensand altered its gut microbial community structure after 6 h. The inhibition of boring length from 9 mg/g α-pinene in diets ceased after 48 h. No significant differences of the bacterial communities were observed between the beetles in 0 and 9 mg/g α-pinene concentration in phloem media after 48 h. Our results showed that the inhibition of the feeding behavior ofD. valensand the disturbance to its gut bacterial communities in 9 mg/g α-pinene concentration in phloem media after 6 h were eliminated after 48 h. The resilience of gut bacterial community ofD. valensmay help the beetle catabolize pine defense chemical.
State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, No. 1 Beichen West Road, Chaoyang District, Beijing 100101, China. letian0926@163.com.|TEDA Institute of Biological Sciences and Biotechnology, Nankai University, TEDA, Tianjin 300457, China. letian0926@163.com.|Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, Tianjin 300071, China. letian0926@163.com.
Letian
L
Xu
Fujian Provincial Key Laboratory of Insect Ecology, College of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou 350002, China. shizh@fafu.edu.cn.
Zhanghong
Z
Shi
Key Laboratory of Tropical Forest Ecology, Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, Menglun 666303, China. vincentwb@126.com.
Bo
B
Wang
State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, No. 1 Beichen West Road, Chaoyang District, Beijing 100101, China. lumin@ioz.ac.cn.
Min
M
Lu
State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, No. 1 Beichen West Road, Chaoyang District, Beijing 100101, China. sunjh@ioz.ac.cn.
Jianghua
J
Sun
D000818:Animals; D001419:Bacteria / Q000145:classification / Q000235:genetics; D000081008:Bicyclic Monoterpenes; D001517:Coleoptera / Q000187:drug effects* / Q000382:microbiology / Q000502:physiology; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004305:Dose-Response Relationship, Drug; D005247:Feeding Behavior / Q000187:drug effects*; D000069196:Gastrointestinal Microbiome / Q000187:drug effects* / Q000235:genetics / Q000502:physiology; D014644:Genetic Variation; D054884:Host-Pathogen Interactions / Q000187:drug effects; D039821:Monoterpenes / Q000494:pharmacology*; D052585:Phloem / Q000737:chemistry; D028223:Pinus / Q000737:chemistry*; D016133:Polymerase Chain Reaction; D025341:Principal Component Analysis; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D013997:Time Factors
D000081008:Bicyclic Monoterpenes; D004269:DNA, Bacterial; D039821:Monoterpenes; D012336:RNA, Ribosomal, 16S; C005451:alpha-pinene
Dendroctonus valens; gut microbiota; α-pinene