Genomic Analysis of Phylotype I Strain EP1 Reveals Substantial Divergence from Other Strains in theRalstonia solanacearumSpecies Complex.
Ralstonia solanacearumspecies complex is a devastating group of phytopathogens with an unusually wide host range and broad geographical distribution.R. solanacearumisolates may differ considerably in various properties including host range and pathogenicity, but the underlying genetic bases remain vague. Here, we conducted the genome sequencing of strain EP1 isolated from Guangdong Province of China, which belongs to phylotype I and is highly virulent to a range of solanaceous crops. Its complete genome contains a 3.95-Mb chromosome and a 2.05-Mb mega-plasmid, which is considerably bigger than reported genomes of otherR. solanacearumstrains. Both the chromosome and the mega-plasmid have essential house-keeping genes and many virulence genes. Comparative analysis of strain EP1 with other 3 phylotype I and 3 phylotype II, III, IV strains unveiled substantial genome rearrangements, insertions and deletions. Genome sequences are relatively conserved among the 4 phylotype I strains, but more divergent among strains of different phylotypes. Moreover, the strains exhibited considerable variations in their key virulence genes, including those encoding secretion systems and type III effectors. Our results provide valuable information for further elucidation of the genetic basis of diversified virulences and host range ofR. solanacearumspecies.
Guangdong Province Key Laboratory of Microbial Signals and Disease Control, State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Integrative Microbiology Research Centre, College of Agriculture, South China Agricultural University Guangzhou, China.
Peng
P
Li
Guangdong Province Key Laboratory of Microbial Signals and Disease Control, State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Integrative Microbiology Research Centre, College of Agriculture, South China Agricultural University Guangzhou, China.
Dechen
D
Wang
Guangdong Province Key Laboratory of Microbial Signals and Disease Control, State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Integrative Microbiology Research Centre, College of Agriculture, South China Agricultural University Guangzhou, China.
Jinli
J
Yan
Guangdong Province Key Laboratory of Microbial Signals and Disease Control, State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Integrative Microbiology Research Centre, College of Agriculture, South China Agricultural University Guangzhou, China.
Jianuan
J
Zhou
Guangdong Province Key Laboratory of Microbial Signals and Disease Control, State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Integrative Microbiology Research Centre, College of Agriculture, South China Agricultural UniversityGuangzhou, China; Guangdong Innovative and Entepreneurial Research Team of Sociomicrobiology Basic Science and Frontier Technology, College of Agriculture, South China Agricultural UniversityGuangzhou, China.
Yinyue
Y
Deng
Guangdong Province Key Laboratory of Microbial Signals and Disease Control, State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Integrative Microbiology Research Centre, College of Agriculture, South China Agricultural University Guangzhou, China.
Zide
Z
Jiang
Department of Vegetables, College of Horticulture, South China Agricultural University Guangzhou, China.
Bihao
B
Cao
Plant Protection Research Institute Guangdong Academy of Agriculture Sciences Guangzhou, China.
Zifu
Z
He
Guangdong Province Key Laboratory of Microbial Signals and Disease Control, State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Integrative Microbiology Research Centre, College of Agriculture, South China Agricultural UniversityGuangzhou, China; Institute of Molecular and Cell BiologySingapore, Singapore.
Lianhui
L
Zhang
Ralstonia solanacearum; comparative genomics; genome dynamics; genome sequencing; virulence