The endotracheal tube microbiome associated with Pseudomonas aeruginosa or Staphylococcus epidermidis.
Ventilator-associated pneumonia (VAP) is one of the commonest hospital-acquired infections associated with high mortality. VAP pathogenesis is closely linked to organisms colonizing the endotracheal tube (ETT) such as Staphylococcus epidermidis and Pseudomonas aeruginosa, the former a common commensal with pathogenic potential and the latter a known VAP pathogen. However, recent gut microbiome studies show that pathogens rarely function alone. Hence, we determined the ETT microbial consortium co-colonizing with S. epidermidis or P. aeruginosa to understand its importance in the development of VAP and for patient prognosis. Using bacterial 16S rRNA and fungal ITS-II sequencing on ETT biomass showing presence of P. aeruginosa and/or S. epidermidis on culture, we found that presence of P. aeruginosa correlated inversely with patient survival and with bacterial species diversity. A decision tree, using 16S rRNA and patient parameters, to predict patient survival was generated. Patients with a relative abundance of Pseudomonadaceae <4.6% and of Staphylococcaceae <70.8% had the highest chance of survival. When Pseudomonadaceae were >4.6%, age of patient <66.5 years was the most important predictor of patient survival. These data indicate that the composition of the ETT microbiome correlates with patient prognosis, and presence of P. aeruginosa is an important predictor of patient outcome.
Laboratory of Medical Microbiology, University of Antwerp, Wilrijk, Belgium.|Vaccine &Infectious Disease Institute, University of Antwerp, Wilrijk, Belgium.|University of Antwerp, Wilrijk, Belgium.
An
A
Hotterbeekx
Laboratory of Medical Microbiology, University of Antwerp, Wilrijk, Belgium.|Vaccine &Infectious Disease Institute, University of Antwerp, Wilrijk, Belgium.|University of Antwerp, Wilrijk, Belgium.
Basil B
BB
Xavier
Laboratory of Medical Microbiology, University of Antwerp, Wilrijk, Belgium.|University of Antwerp, Wilrijk, Belgium.|Molecular Pathology group, Cell Biology and Histology, University of Antwerp, Wilrijk, Belgium.
Kenny
K
Bielen
Laboratory of Medical Microbiology, University of Antwerp, Wilrijk, Belgium.|Vaccine &Infectious Disease Institute, University of Antwerp, Wilrijk, Belgium.|University of Antwerp, Wilrijk, Belgium.
Christine
C
Lammens
Laboratory of Medical Microbiology, University of Antwerp, Wilrijk, Belgium.|Vaccine &Infectious Disease Institute, University of Antwerp, Wilrijk, Belgium.|University of Antwerp, Wilrijk, Belgium.
Pieter
P
Moons
Critical Care Unit, Antwerp University Hospital, Edegem, Belgium.|Antwerp University Hospital, Edegem, Belgium.
Tom
T
Schepens
Laboratory of Medical Microbiology, University of Antwerp, Wilrijk, Belgium.|Vaccine &Infectious Disease Institute, University of Antwerp, Wilrijk, Belgium.|University of Antwerp, Wilrijk, Belgium.|Antwerp University Hospital, Edegem, Belgium.
Margareta
M
Ieven
University of Antwerp, Wilrijk, Belgium.|Critical Care Unit, Antwerp University Hospital, Edegem, Belgium.|Antwerp University Hospital, Edegem, Belgium.
Philippe G
PG
Jorens
Laboratory of Medical Microbiology, University of Antwerp, Wilrijk, Belgium.|Vaccine &Infectious Disease Institute, University of Antwerp, Wilrijk, Belgium.|University of Antwerp, Wilrijk, Belgium.|Antwerp University Hospital, Edegem, Belgium.
Herman
H
Goossens
Laboratory of Medical Microbiology, University of Antwerp, Wilrijk, Belgium.|University of Antwerp, Wilrijk, Belgium.|Molecular Pathology group, Cell Biology and Histology, University of Antwerp, Wilrijk, Belgium.
Samir
S
Kumar-Singh
Laboratory of Medical Microbiology, University of Antwerp, Wilrijk, Belgium.|Vaccine &Infectious Disease Institute, University of Antwerp, Wilrijk, Belgium.|University of Antwerp, Wilrijk, Belgium.
Surbhi
S
Malhotra-Kumar
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D018441:Biofilms / Q000254:growth & development; D005260:Female; D006801:Humans; D007442:Intubation, Intratracheal / Q000009:adverse effects*; D008297:Male; D064307:Microbiota / Q000235:genetics; D008875:Middle Aged; D053717:Pneumonia, Ventilator-Associated / Q000209:etiology* / Q000382:microbiology*; D011552:Pseudomonas Infections / Q000209:etiology* / Q000382:microbiology; D011550:Pseudomonas aeruginosa / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D013203:Staphylococcal Infections / Q000209:etiology* / Q000382:microbiology; D013212:Staphylococcus epidermidis / Q000235:genetics
D012336:RNA, Ribosomal, 16S