Exploring viral infection using single-cell sequencing.
Single-cell sequencing (SCS) has emerged as a valuable tool to study cellular heterogeneity in diverse fields, including virology. By studying the viral and cellular genome and/or transcriptome, the dynamics of viral infection can be investigated at single cell level. Most studies have explored the impact of cell-to-cell variation on the viral life cycle from the point of view of the virus, by analyzing viral sequences, and from the point of view of the cell, mainly by analyzing the cellular host transcriptome. In this review, we will focus on recent studies that use single-cell sequencing to explore viral diversity and cell variability in response to viral replication.
Institute of Microbiology, University Hospital Center and University of Lausanne, Bugnon 48, CH-1011 Lausanne, Switzerland. Electronic address: Sylvie.Ferreira-Rato@chuv.ch.
Sylvie
S
Rato
Department of Biosystems Science and Engineering, ETH Zürich and SIB Swiss Institute of Bioinformatics, CH-4058 Basel, Switzerland. Electronic address: monica.golumbeanu@bsse.ethz.ch.
Monica
M
Golumbeanu
The J. Craig Venter Institute, 4120 Capricorn Lane, La Jolla, CA 92037, USA. Electronic address: atelenti@jcvi.org.
Amalio
A
Telenti
Institute of Microbiology, University Hospital Center and University of Lausanne, Bugnon 48, CH-1011 Lausanne, Switzerland. Electronic address: Angela.Ciuffi@chuv.ch.
Angela
A
Ciuffi
D000818:Animals; D019295:Computational Biology / Q000379:methods; D044127:Epigenesis, Genetic; D020869:Gene Expression Profiling; D023281:Genomics / Q000379:methods; D059014:High-Throughput Nucleotide Sequencing; D054884:Host-Pathogen Interactions / Q000235:genetics*; D006801:Humans; D059010:Single-Cell Analysis / Q000379:methods; D014777:Virus Diseases / Q000235:genetics* / Q000821:virology*; D018406:Virus Physiological Phenomena
Heterogeneity; Single-cell; Single-cell sequencing; Viral infection; Virus