Transcriptional Analysis of serk1 and serk3 Coreceptor Mutants.
Somatic embryogenesis receptor kinases (SERKs) are ligand-binding coreceptors that are able to combine with different ligand-perceiving receptors such as BRASSINOSTEROID INSENSITIVE1 (BRI1) and FLAGELLIN-SENSITIVE2. Phenotypical analysis of serk single mutants is not straightforward because multiple pathways can be affected, while redundancy is observed for a single phenotype. For example, serk1serk3 double mutant roots are insensitive toward brassinosteroids but have a phenotype different from bri1 mutant roots. To decipher these effects, 4-d-old Arabidopsis (Arabidopsis thaliana) roots were studied using microarray analysis. A total of 698 genes, involved in multiple biological processes, were found to be differentially regulated in serk1-3serk3-2 double mutants. About half of these are related to brassinosteroid signaling. The remainder appear to be unlinked to brassinosteroids and related to primary and secondary metabolism. In addition, methionine-derived glucosinolate biosynthesis genes are up-regulated, which was verified by metabolite profiling. The results also show that the gene expression pattern in serk3-2 mutant roots is similar to that of the serk1-3serk3-2 double mutant roots. This confirms the existence of partial redundancy between SERK3 and SERK1 as well as the promoting or repressive activity of a single coreceptor in multiple simultaneously active pathways.
Laboratory of Biochemistry, Wageningen University, 6708 WE Wageningen, The Netherlands.
G Wilma
0000-0001-5012-2346
GW
van Esse
Laboratory of Biochemistry, Wageningen University, 6708 WE Wageningen, The Netherlands.
Colette A
0000-0002-6110-2209
CA
Ten Hove
Laboratory of Biochemistry, Wageningen University, 6708 WE Wageningen, The Netherlands.
Francesco
0000-0002-6953-0523
F
Guzzonato
Laboratory of Biochemistry, Wageningen University, 6708 WE Wageningen, The Netherlands.
H Peter
0000-0002-3667-060X
HP
van Esse
Laboratory of Biochemistry, Wageningen University, 6708 WE Wageningen, The Netherlands.
Mark
M
Boekschoten
Laboratory of Biochemistry, Wageningen University, 6708 WE Wageningen, The Netherlands.
Lars
L
Ridder
Laboratory of Biochemistry, Wageningen University, 6708 WE Wageningen, The Netherlands.
Jacques
J
Vervoort
Laboratory of Biochemistry, Wageningen University, 6708 WE Wageningen, The Netherlands sacco.devries@wur.nl.
Sacco C
0000-0001-9454-775X
SC
de Vries
D000483:Alleles; D017360:Arabidopsis / Q000235:genetics*; D029681:Arabidopsis Proteins / Q000235:genetics*; D060406:Brassinosteroids / Q000378:metabolism; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D005961:Glucosinolates / Q000494:pharmacology; D055442:Metabolome / Q000187:drug effects; D015999:Multivariate Analysis; D009154:Mutation / Q000235:genetics*; D010641:Phenotype; D018517:Plant Roots / Q000187:drug effects / Q000235:genetics; D011494:Protein Kinases / Q000235:genetics*; D017346:Protein Serine-Threonine Kinases / Q000235:genetics*; D014158:Transcription, Genetic / Q000187:drug effects
D029681:Arabidopsis Proteins; D060406:Brassinosteroids; D005961:Glucosinolates; D011494:Protein Kinases; C463660:BAK1 protein, Arabidopsis; C440035:SERK1 protein, Arabidopsis; D017346:Protein Serine-Threonine Kinases