Independent modes of ganglion cell translocation ensure correct lamination of the zebrafish retina.
The arrangement of neurons into distinct layers is critical for neuronal connectivity and function. During development, most neurons move from their birthplace to the appropriate layer, where they polarize. However, kinetics and modes of many neuronal translocation events still await exploration. In this study, we investigate retinal ganglion cell (RGC) translocation across the embryonic zebrafish retina. After completing their translocation, RGCs establish the most basal retinal layer where they form the optic nerve. Using in toto light sheet microscopy, we show that somal translocation of RGCs is a fast and directed event. It depends on basal process attachment and stabilized microtubules. Interestingly, interference with somal translocation induces a switch to multipolar migration. This multipolar mode is less efficient but still leads to successful RGC layer formation. When both modes are inhibited though, RGCs fail to translocate and induce lamination defects. This indicates that correct RGC translocation is crucial for subsequent retinal lamination.
Max Planck Institute of Molecular Cell Biology and Genetics, 01307 Dresden, Germany.
Jaroslav
http://orcid.org/0000-0002-0657-3278
J
Icha
Max Planck Institute of Molecular Cell Biology and Genetics, 01307 Dresden, Germany.
Christiane
http://orcid.org/0000-0002-1280-6998
C
Kunath
Max Planck Institute of Molecular Cell Biology and Genetics, 01307 Dresden, Germany.|Instituto de Biofísica Carlos Chagas Filho, 21941-902 Rio de Janeiro, Brazil.
Mauricio
http://orcid.org/0000-0001-6881-6733
M
Rocha-Martins
Max Planck Institute of Molecular Cell Biology and Genetics, 01307 Dresden, Germany norden@mpi-cbg.de.
Caren
http://orcid.org/0000-0001-8835-1451
C
Norden
D051376:Actin-Related Protein 2-3 Complex / Q000378:metabolism; D000818:Animals; D002454:Cell Differentiation; D002465:Cell Movement; D002467:Cell Nucleus / Q000378:metabolism; D002470:Cell Survival; D004625:Embryo, Nonmammalian / Q000166:cytology; D007700:Kinetics; D008870:Microtubules / Q000378:metabolism; D008954:Models, Biological; D015388:Organelles / Q000378:metabolism; D012165:Retinal Ganglion Cells / Q000166:cytology*; D013234:Stem Cells / Q000166:cytology / Q000378:metabolism; D015027:Zebrafish / Q000196:embryology / Q000378:metabolism*
D051376:Actin-Related Protein 2-3 Complex