Experimental model of glucocorticoid-induced insulin resistance.
To evaluate metabolic effects in experimental model of glucocorticoid-induced insulin resistance.
Twenty Wistar male rats were randomly divided into two groups, which were treated with intraperitoneally injected dexamethasone 1mg/Kg/day for ten days consecutively (Group D; n=10) and placebo (Group C; n=10). The variables analyzed were: from the first to the 10th day - body weight (before and after treatment); food and water daily consumption; on the 10th day - glycemia, insulinemia, HOMA-beta and HOMA-IR. The blood samples for laboratory analysis were obtained by intracardiac puncture. Also on the 10th day liver fragments were taken for analyzing glycogen and fattty.
Group D animals compared to group C had: weight reduction (g), (D=226.5±24.7 vs C=295.0±25.4; p=0.001); increased glycemia (mmol/l) (D=19.5±2.1 vs C=14.2±3.1; p=0.0001); diminished insulinemia (mU/l) (D=0.2±0.1 vs C=2.0±0.4; p=0.0001); reduced HOMA-β (D=0.2±0.1 vs C=4.2±1.7; p=0.0002); diminished HOMA-IR (D=0.2±0.1 vs C=1.3±0.4; p=0.0002). Histological examination of the liver showed that 100% of group D and none of group C had moderate fatty. (p=0.2).
Animals treated with glucocorticoid, in this experimental model, expressed hyperglycemia, hypoinsulinism and decreased peripheral insulin sensitivity.
Full Professor, Medical Physiology Division, School of Medicine, Postgraduate Program in Sciences Applied to Health, Universidade do Vale do Sapucaí (UNIVAS), Pouso Alegre-MG, Brazil. Conception and design of the study; acquisition, analysis and interpretation of data; manuscript writing; critical revision.
Beatriz Bertolaccini
BB
Martínez
Graduate student, School of Medicine, UNIVAS, Pouso Alegre-MG, Brazil. Acquisition of data.
Ana Carolina Couto
AC
Pereira
Graduate student, School of Medicine, UNIVAS, Pouso Alegre-MG, Brazil. Acquisition of data.
Júlio Henrique
JH
Muzetti
Graduate student, School of Medicine, UNIVAS, Pouso Alegre-MG, Brazil. Acquisition of data.
Fernando de Paiva
FP
Telles
Associate Professor, Department of Pathology, School of Medicine, UNIVAS, Pouso Alegre-MG, Brazil. Histopathological examinations.
Fiorita Gonzáles Lopes
FG
Mundim
Full Professor, Biological Sciences Department, Postgraduate Program in Sciences Applied to Health, UNIVAS, Pouso Alegre-MG, Brazil. Conception and design of the study.
Manoel Araújo
MA
Teixeira
D000818:Animals; D001786:Blood Glucose / Q000032:analysis; D001835:Body Weight; D003907:Dexamethasone / Q000009:adverse effects*; D003920:Diabetes Mellitus / Q000139:chemically induced; D004195:Disease Models, Animal; D005938:Glucocorticoids / Q000009:adverse effects*; D006706:Homeostasis / Q000187:drug effects; D006943:Hyperglycemia / Q000139:chemically induced; D007333:Insulin Resistance; D008099:Liver / Q000187:drug effects; D008297:Male; D011897:Random Allocation; D017208:Rats, Wistar
D001786:Blood Glucose; D005938:Glucocorticoids; D003907:Dexamethasone