UHPLC-MS/MS determination and pharmacokinetic study of plantamajoside in rat plasma after oral administration of single plantamajoside and Plantago asiatica extract.
A sensitive and reliable ultra-high performance liquid chromatography coupled with tandem quadrupole mass spectrometry (UHPLC-MS/MS) method was developed for quantitation of plantamajoside in rat plasma. First, this study compared the pharmacokinetic properties of plantamajoside after oral administration of Plantago asiatica extract and pure plantamajoside in rat plasma with approximately the same dosage of 8.98 mg/kg. Second, chromatographic separation was performed on an Acquity HSS C18column (50 × 2.1 mm, p.d.1.7 μm) with isocratic elution using methanol-water (80:20, v/v) as mobile phase at a flow rate of 0.25 mL/min. The calibration curves were linear over the range of 0.1-100 ng/mL for plantamajoside. At different time points (0, 0.083, 0.167, 0.25, 0.5, 0.75, 1, 2, 3, 4, 5, 6 and 8 h) after administration, the concentrations of plantamajoside in plasma were measured and the main pharmacokinetic parameters were estimated. The study indicates that the pharmacokinetics of plantamajoside in rat plasma have significant differences between two groups.
Department of Pathology, Health Science Center, Xi'an Jiaotong University, Xi'an, China.|Department of Emergency, Zhongshan Hospital of Dalian University, Dalian, China.
Lizhi
L
Bai
Department of General Surgery, The First People's Hospital of Jinzhou District in Dalian City, Dalian, China.
Li
L
Han
Department of Emergency, Zhongshan Hospital of Dalian University, Dalian, China.
Xiaoguang
X
Lu
Department of Emergency, Zhongshan Hospital of Dalian University, Dalian, China.
Xin
X
Kang
Department of Emergency, Zhongshan Hospital of Dalian University, Dalian, China.
Zhiwei
Z
Fan
Department of Pathology and Pathophysiology, Dalian Medical University, Dalian, China.
Rong
R
Xing
Department of Pathology, Health Science Center, Xi'an Jiaotong University, Xi'an, China.
Dangxia
D
Zhou
D000284:Administration, Oral; D000818:Animals; D002396:Catechols / Q000008:administration & dosage / Q000097:blood* / Q000493:pharmacokinetics*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004355:Drug Stability; D005960:Glucosides / Q000008:administration & dosage / Q000097:blood* / Q000493:pharmacokinetics*; D057230:Limit of Detection; D008297:Male; D010936:Plant Extracts / Q000008:administration & dosage / Q000493:pharmacokinetics*; D010943:Plantago / Q000737:chemistry*; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D052616:Solid Phase Extraction; D053719:Tandem Mass Spectrometry / Q000379:methods
D002396:Catechols; D005960:Glucosides; D010936:Plant Extracts; C421173:plantamajoside
Plantago asiatica extract; UHPLC-MS/MS; comparative pharmacokinetics; plantamajoside; rat plasma