Construction and Rescue of a Molecular Clone of Deformed Wing Virus (DWV).
European honey bees are highly important in crop pollination, increasing the value of global agricultural production by billions of dollars. Current knowledge about virulence and pathogenicity of Deformed wing virus (DWV), a major factor in honey bee colony mortality, is limited. With this study, we close the gap between field research and laboratory investigations by establishing a complete in vitro model for DWV pathogenesis. Infectious DWV was rescued from a molecular clone of a DWV-A genome that induces DWV symptoms such as crippled wings and discoloration. The expression of DWV proteins, production of infectious virus progeny, and DWV host cell tropism could be confirmed using newly generated anti-DWV monoclonal antibodies. The recombinant RNA fulfills Koch's postulates circumventing the need of virus isolation and propagation of pure virus cultures. In conclusion, we describe the development and application of a reverse genetics system for the study of DWV pathogenesis.
Institute of Virology, Department of Pathobiology, University of Veterinary Medicine, Vienna, Austria.
Benjamin
B
Lamp
Institute of Pathology and Forensic Veterinary Medicine, Department of Pathobiology, University of Veterinary Medicine, Vienna, Austria.
Angelika
A
Url
Institute of Virology, Department of Pathobiology, University of Veterinary Medicine, Vienna, Austria.
Kerstin
K
Seitz
Institute of Virology, Department of Pathobiology, University of Veterinary Medicine, Vienna, Austria.
Jürgen
J
Eichhorn
Institute of Virology, Department of Pathobiology, University of Veterinary Medicine, Vienna, Austria.
Christiane
C
Riedel
Institute of Virology, Department of Pathobiology, University of Veterinary Medicine, Vienna, Austria.
Leonie Janina
LJ
Sinn
Institute of Virology, Department of Pathobiology, University of Veterinary Medicine, Vienna, Austria.
Stanislav
S
Indik
Institute for Apiculture, Agricultural Inspection Service and Research Centre Vienna, Austrian Agency for Health and Food Safety and Federal Office for Food Safety, Vienna, Austria.
Hemma
H
Köglberger
Institute of Virology, Department of Pathobiology, University of Veterinary Medicine, Vienna, Austria.
Till
T
Rümenapf
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D001483:Base Sequence; D001516:Bees / Q000821:virology; D015153:Blotting, Western; D036022:Capsid Proteins / Q000276:immunology; D016679:Genome, Viral / Q000235:genetics; D054884:Host-Pathogen Interactions; D007150:Immunohistochemistry; D007304:Insect Viruses / Q000235:genetics* / Q000378:metabolism / Q000502:physiology; D008807:Mice, Inbred BALB C; D046529:Microscopy, Electron, Transmission; D010802:Phylogeny; D010849:Picornaviridae / Q000145:classification / Q000235:genetics* / Q000378:metabolism; D020815:Polyproteins / Q000235:genetics / Q000378:metabolism; D011679:Pupa / Q000821:virology; D012328:RNA Viruses / Q000235:genetics* / Q000378:metabolism / Q000648:ultrastructure; D012367:RNA, Viral / Q000235:genetics* / Q000302:isolation & purification; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D014764:Viral Proteins / Q000378:metabolism; D014921:Wings, Animal / Q000821:virology
D000911:Antibodies, Monoclonal; D036022:Capsid Proteins; D020815:Polyproteins; D012367:RNA, Viral; D014764:Viral Proteins