Active antioxidants in ex-vivo examination of burn wound healing by means of IR and Raman spectroscopies-Preliminary comparative research.
Being a complex traumatic event, burn injury also affects other organ systems apart from the skin. Wounds undergo various pathological changes which are accompanied by alterations in the molecular environment. Information about molecules may be obtained with the use of Raman spectroscopy and Fourier-transform infrared spectroscopy, and when combined, both methods are a powerful tool for providing material characterization. Alterations in the molecular environment may lead to identifying objective markers of acute wound healing. In general, incubation of samples in solutions of l-ascorbic acid and 5% and 7% orthosilicic acid organizes the collagen structure, whereas the increased intensity of the Raman bands in the region of 1500-800cm-1reveals regeneration of the burn tissue. Since oxidative damage is one of the mechanisms responsible for local and distant pathophysiological events after burn, antioxidant therapy can prove to be beneficial in minimizing burn wounds, which was examined on the basis of human skin samples and chicken skin samples, the latter being subject to modification when heated to a temperature sufficient for the simulation of a burn incident.
Faculty of Materials, Civil and Environmental Engineering, University of Bielsko-Biała, 2 Willowa St., 43-300 Bielsko-Biała, Poland. Electronic address: apielesz@ath.bielsko.pl.
Anna
A
Pielesz
Faculty of Materials, Civil and Environmental Engineering, University of Bielsko-Biała, 2 Willowa St., 43-300 Bielsko-Biała, Poland.
Dorota
D
Biniaś
Faculty of Materials, Civil and Environmental Engineering, University of Bielsko-Biała, 2 Willowa St., 43-300 Bielsko-Biała, Poland.
Ewa
E
Sarna
Faculty of Health Sciences, University of Bielsko-Biała, Poland.
Rafał
R
Bobiński
Faculty of Health Sciences, University of Bielsko-Biała, Poland; Dr Stanislaw Sakiel Center for Burns Treatment in Siemianowice Śląskie, 2 Jana Pawła II St., 41-100 Siemianowice Śląskie, Poland.
Marek
M
Kawecki
Dr Stanislaw Sakiel Center for Burns Treatment in Siemianowice Śląskie, 2 Jana Pawła II St., 41-100 Siemianowice Śląskie, Poland; Unit for Chronic Wound Treatment Organization, Division of Nursery, School of Health Care, Medical University of Silesia, 2 Jana Pawła II St., 41-100 Siemianowice Śląskie, Poland.
Justyna
J
Glik
Dr Stanislaw Sakiel Center for Burns Treatment in Siemianowice Śląskie, 2 Jana Pawła II St., 41-100 Siemianowice Śląskie, Poland.
Agnieszka
A
Klama-Baryła
Dr Stanislaw Sakiel Center for Burns Treatment in Siemianowice Śląskie, 2 Jana Pawła II St., 41-100 Siemianowice Śląskie, Poland.
Diana
D
Kitala
Dr Stanislaw Sakiel Center for Burns Treatment in Siemianowice Śląskie, 2 Jana Pawła II St., 41-100 Siemianowice Śląskie, Poland.
Wojciech
W
Łabuś
Faculty of Materials, Civil and Environmental Engineering, University of Bielsko-Biała, 2 Willowa St., 43-300 Bielsko-Biała, Poland.
Jadwiga
J
Paluch
Dr Stanislaw Sakiel Center for Burns Treatment in Siemianowice Śląskie, 2 Jana Pawła II St., 41-100 Siemianowice Śląskie, Poland.
Małgorzata
M
Kraut
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D002056:Burns / Q000188:drug therapy / Q000378:metabolism / Q000473:pathology; D002645:Chickens; D006801:Humans; D012867:Skin / Q000378:metabolism / Q000473:pathology; D013055:Spectrophotometry, Infrared; D013059:Spectrum Analysis, Raman; D014945:Wound Healing / Q000187:drug effects*
D000975:Antioxidants
Burn wound healing; Hydrogels e.g. H(4)SiO(4)×nH(2)O (5% and 7%); l-ascorbic acid