Impact of a pesticide cocktail (fenhexamid, folpel, deltamethrin) on the abundance of Glomeromycota in two agricultural soils.
Pesticide contamination of the environment can result from agricultural practices. Persistence of pesticide residues is a threat to the soil biota including plant roots and beneficial microorganisms, which support an important number of soil ecosystem services. Arbuscular mycorrhizal fungi (AMF) are key symbiotic microorganisms contributing to plant nutrition. In the present study, we assessed whether AMF could indicate eventual side effects of pesticides when directly applied to field soils. We evaluated the ecotoxicological impact of a cocktail of three commonly used agricultural pesticides (fenhexamid, folpel, deltamethrin) on the abundance and composition of the AMF community in vineyard (Montagne de Saint-Emilion) and arable (Martincourt) soils subjected to different agricultural practices. The dissipation of applied pesticides was monitored by multiresidual analyses to determine the scenario of exposure of the AMF community. Diversity analysis before application of the pesticide cocktail showed that the AMF communities of vineyard soils, subjected to mechanical weeding or grass cover, and of the arable soil subjected to intensive agriculture, were dominated by Glomerales. Ribotypes specific to each soil and to each agricultural practice in the same soil were found, with the highest abundance and diversity of AMF being observed in the vineyard soil with a grass-cover. The abundance of the global AMF community (Glomeromycota) and of three taxa of AMF (Funneliformis mosseae, Claroideoglomus etunicatum/C. claroideum) was evaluated after pesticide application. The abundance of Glomeromycota decreased in both soils after pesticide application while the abundance of Claroideoglomus and F. mosseae decreased only in the arable soil. These results show that higher doses of pesticide exposure did not affect the global abundance, but altered the composition, of the AMF community. Resilience of the AMF community composition was observed only in the vineyard soil, where F. mosseae was the most tolerant taxon to pesticide exposure.
Agroécologie, AgroSup Dijon, CNRS, INRA, Univ. Bourgogne Franche-Comté, Dijon, France; Agroécologie, AgroSup Dijon, INRA, Univ. Bourgogne Franche-Comté, Dijon, France; Departamento El Hombre y su Ambiente, Universidad Autónoma Metropolitana-Xochimilco, Mexico City, Mexico.
Facundo
F
Rivera-Becerril
Agroécologie, AgroSup Dijon, CNRS, INRA, Univ. Bourgogne Franche-Comté, Dijon, France. Electronic address: diederik.van-tuinen@inra.fr.
Diederik
D
van Tuinen
Agroécologie, AgroSup Dijon, CNRS, INRA, Univ. Bourgogne Franche-Comté, Dijon, France.
Odile
O
Chatagnier
Agroécologie, AgroSup Dijon, INRA, Univ. Bourgogne Franche-Comté, Dijon, France.
Nadine
N
Rouard
Agroécologie, AgroSup Dijon, INRA, Univ. Bourgogne Franche-Comté, Dijon, France.
Jérémie
J
Béguet
Agroécologie, AgroSup Dijon, CNRS, INRA, Univ. Bourgogne Franche-Comté, Dijon, France.
Catherine
C
Kuszala
INRA/Université de Bordeaux 2, UMR Œnologie, Villenave Dornon, France.
Guy
G
Soulas
Agroécologie, AgroSup Dijon, CNRS, INRA, Univ. Bourgogne Franche-Comté, Dijon, France.
Vivienne
V
Gianinazzi-Pearson
Agroécologie, AgroSup Dijon, INRA, Univ. Bourgogne Franche-Comté, Dijon, France.
Fabrice
F
Martin-Laurent
D000577:Amides; D005602:France; D055137:Glomeromycota / Q000145:classification / Q000254:growth & development*; D038821:Mycorrhizae / Q000145:classification / Q000254:growth & development; D009570:Nitriles; D010575:Pesticides / Q000032:analysis*; D011722:Pyrethrins; D012987:Soil / Q000737:chemistry*; D012988:Soil Microbiology
D000577:Amides; D009570:Nitriles; D010575:Pesticides; D011722:Pyrethrins; D012987:Soil; C017180:decamethrin; C451426:N-(2,3-dichloro-4-hydroxyphenyl)-1-methylcyclohexanecarboxamide
Arbuscular mycorrhiza; Deltamethrin; Fenhexamid; Folpel; Glomeromycetes; Vineyard