Biodegradation of crude oil by a defined co-culture of indigenous bacterial consortium and exogenous Bacillus subtilis.
The aim of this work was to study biodegradation of crude oil by defined co-cultures of indigenous bacterial consortium and exogenous Bacillus subtilis. Through residual oil analysis, it is apparent that the defined co-culture displayed a degradation ratio (85.01%) superior to indigenous bacterial consortium (71.32%) after 7days of incubation when ratio of inoculation size of indigenous bacterial consortium and Bacillus subtilis was 2:1. Long-chain n-alkanes could be degraded markedly by Bacillus subtilis. Result analysis of the bacterial community showed that a decrease in bacterial diversity in the defined co-culture and the enrichment of Burkholderiales order (98.1%) degrading hydrocarbons. The research results revealed that the promising potential of the defined co-culture for application to degradation of crude oil.
Laboratory of Environmental Remediation, College of Environmental and Chemical Engineering, Shanghai University, Shanghai 200444, China.
Kaiyun
K
Tao
Laboratory of Environmental Remediation, College of Environmental and Chemical Engineering, Shanghai University, Shanghai 200444, China. Electronic address: Lxy999@shu.edu.cn.
Xiaoyan
X
Liu
Laboratory of Environmental Remediation, College of Environmental and Chemical Engineering, Shanghai University, Shanghai 200444, China.
Xueping
X
Chen
Laboratory of Environmental Remediation, College of Environmental and Chemical Engineering, Shanghai University, Shanghai 200444, China.
Xiaoxin
X
Hu
Laboratory of Environmental Remediation, College of Environmental and Chemical Engineering, Shanghai University, Shanghai 200444, China.
Liya
L
Cao
Laboratory of Environmental Remediation, College of Environmental and Chemical Engineering, Shanghai University, Shanghai 200444, China.
Xiaoyu
X
Yuan
D001412:Bacillus subtilis / Q000378:metabolism*; D001673:Biodegradation, Environmental; D006838:Hydrocarbons / Q000378:metabolism*; D010578:Petroleum / Q000378:metabolism*
D006838:Hydrocarbons; D010578:Petroleum
Bacillus subtili; Biodegradation; Co-culture of bacteria; Crude oil; High throughput sequencing