Laminin-Alginate Beads as Preadipocyte Carriers to Enhance Adipogenesis In Vitro and In Vivo.
The use of autologous fat grafting in breast reconstruction still requires optimization. Fat survival and calcification are the main issues that affect the outcomes of the procedure. In this study, a cell-based therapy utilizing laminin-alginate beads (LABs) as carriers was proposed to promote cell survival and adipogenesis by providing short-term physical support and facilitate nutrient diffusion of the implants. Laminin-modified alginate beads were fabricated by immobilizing laminin onto ring-opened alginate, used to encapsulate 3T3-L1 preadipocytes, and evaluated in vitro and in vivo. LABs as preadipocyte carriers showed better biocompatibility and stability than unmodified alginate beads. Preadipocytes in LABs had higher survival rate and enhanced adipogenesis than those in unmodified alginate beads. In vivo studies showed that LABs gradually degraded and the sites were replaced by newly formed fat tissues, and new blood vessels were also observed. 7T-MRI study mimicking clinical fat grafting showed that LABs carrying adipose stem cells improved the results of conventional fat grafts. Therefore, we believe that LABs represent promising cell carriers and can be potentially used for the reconstruction of breasts or other soft tissues in the future.
1 Institute of Biomedical Engineering, National Taiwan University , Taipei, Taiwan .|2 Taiwan Food and Drug Administration, Ministry of Health and Welfare , Taipei, Taiwan .
Yu-Sheng
YS
Hsueh
1 Institute of Biomedical Engineering, National Taiwan University , Taipei, Taiwan .|3 Department of Plastic Surgery, Far Eastern Memorial Hospital , New Taipei City, Taiwan .
Yo-Shen
YS
Chen
4 Department of Plastic Surgery, National Taiwan University Hospital , Taipei, Taiwan .
Hao-Chih
HC
Tai
5 Department of Plastic Surgery, First Faculty of Medicine, Charles University in Prague , Bulovka Hospital, Prague, Czech Republic .
Ondrej
O
Mestak
6 Department of Surgery, National Taiwan University Hospital , Taipei, Taiwan .
Sung-Chuan
SC
Chao
1 Institute of Biomedical Engineering, National Taiwan University , Taipei, Taiwan .
Yen-Yu
YY
Chen
1 Institute of Biomedical Engineering, National Taiwan University , Taipei, Taiwan .
Ying
Y
Shih
1 Institute of Biomedical Engineering, National Taiwan University , Taipei, Taiwan .
Jung-Feng
JF
Lin
1 Institute of Biomedical Engineering, National Taiwan University , Taipei, Taiwan .
Ming-Jium
MJ
Shieh
1 Institute of Biomedical Engineering, National Taiwan University , Taipei, Taiwan .|7 Division of Medical Engineering, National Health Research Institute , Maoli, Taiwan .
Feng-Huei
FH
Lin
D041721:3T3-L1 Cells; D017667:Adipocytes / Q000378:metabolism / Q000637:transplantation; D050156:Adipogenesis; D000464:Alginates / Q000737:chemistry*; D000818:Animals; D018914:Cells, Immobilized / Q000378:metabolism / Q000637:transplantation; D005260:Female; D020723:Glucuronic Acid / Q000737:chemistry; D006603:Hexuronic Acids / Q000737:chemistry; D007797:Laminin / Q000737:chemistry*; D051379:Mice; D016688:Mice, Inbred NOD; D016513:Mice, SCID; D017207:Rats, Sprague-Dawley
D000464:Alginates; D006603:Hexuronic Acids; D007797:Laminin; D020723:Glucuronic Acid
adipogenesis; cell-assisted lipotransfer; fat survival; laminin-alginate bead; preadipocytes