TaqMan Low Density Array: MicroRNA Profiling for Biomarker and Oncosuppressor Discovery.
MicroRNAs (miRNAs) have gained a lot of interest as biomarkers and biotherapeutics in recent years. The discovery of miRNAs in circulation as recently as 2008, aided by rapid advances in high-throughput profiling techniques initiated an explosion of investigations dedicated to discovering circulating miRNAs as minimally invasive biomarkers. As miRNAs regulate many cellular processes, investigators are actively exploring their relevance in disease treatment by miRNA restoration. This chapter demonstrates an approach to discover miRNAs of biomarker and therapeutic potential by isolating miRNAs from cell lines, performing global miRNA profiling, investigating miRNA expression in clinical specimens and examining their therapeutic relevance by restoring miRNA expression using Lipofectamine in vitro.
School of Pharmacy and Pharmaceutical Sciences & Trinity Biomedical Sciences Institute, Trinity College, Dublin, Ireland. obrienk9@tcd.ie.|APC Ltd., Building 11, Cherrywood Business Park, Loughlinstown, Co., Dublin, D18 DH50, Ireland. obrienk9@tcd.ie.
Keith
K
O'Brien
D014408:Biomarkers, Tumor / Q000235:genetics* / Q000378:metabolism; D045744:Cell Line, Tumor; D015870:Gene Expression; D020869:Gene Expression Profiling; D016147:Genes, Tumor Suppressor; D006801:Humans; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction
D014408:Biomarkers, Tumor; D035683:MicroRNAs
Biomarker; Clinical; In vitro; RNAi; TaqMan low density array; Transfection; miRNA mimic; microRNA