Vertical gastrectomy in overweight and non-overweight rats.
To compare the two lines suture (total and seromuscular) after partial gastrectomy in normal and overweight rats.
Forty Wistar rats were distributed in two groups. Group A received normal diet; group B, normal diet and supplementation with saccharose in the water. When group B progressed to a statistically greater weight than the animals of group A, the experiment (sleeve-like gastrectomy) was conducted with gastrorraphy in two sutures lines (total and seromuscular).The animals were distributed into two subgroups of 10. A1 and A2 subgroups were sacrificed at 7 and 14 days postoperatively as well as B1 and B2. Mortality, morbidity, complications attributed to the gastric suture, biochemical dosages, Lee index, macroscopy, weight of retroperitoneal and gonadal fat, optical microscopy with hematoxylin-eosin and picrosirius-red, were the evaluation parameters.
The overweight group achieved statistically greater weight after 16 weeks in induced obesity; there was no mortality or complications with clinical consequences attributable to morbidity. The overweight group had statistically greater weight of gonadal and retroperitoneal fat. The difference was observed in urea, albumin, total cholesterol and indirect bilirubin.
There was no outcome difference between the overweight and non-overweight group in two suture lines in gastrorrhaphy after sleeve-like gastrectomy.
PhD, Associate Professor, Postgraduate Program in Principles of Surgery, Faculdade Evangélica do Paraná (FEPAR), Curitiba-PR, Brazil. Intellectual and scientific content of the study; technical procedures; acquisition, analysis and interpretation of data; manuscript writing.
Fernando Issamu
FI
Tabushi
Fellow Master degree, Postgraduate Program in Surgical Clinic, Universidade Federal do Paraná (UFPR), Curitiba-PR, Brazil. Technical procedures.
Melissa Spena
MS
Stueber
PhD, Associate Professor, Department of Surgical Technique and Experimental Surgery, UFPR, Curitiba-PR, Brazil. Scientific and intellectual content of the study, conception and design of the study, manuscript preparation, critical revision.
Benur
B
Polonio
PhD, Clinics Hospital, UFPR, Curitiba-PR, Brazil. Scientific and intellectual content of the study; conception and design of the study; acquisition, analysis and interpretation of data; technical procedures.
João Carlos Domingues
JC
Repka
PhD, Department of Medical Pathology, UFPR, Curirtiba-PR, Brazil. Histopathological examinations.
Sérgio Ossamu
SO
Ioshii
PhD, Associate Professor, Postgraduate Program in Principles of surgery, FEPAR, Curitiba-PR, Brazil. Conception and design of the study, manuscript writing, critical revision, final approval.
Paulo Afonso Nunes
PA
Nassif
D000818:Animals; D001663:Bilirubin / Q000097:blood; D002784:Cholesterol / Q000097:blood; D005743:Gastrectomy / Q000379:methods*; D007813:Laparotomy / Q000379:methods; D008297:Male; D050177:Overweight / Q000601:surgery*; D011184:Postoperative Period; D017208:Rats, Wistar; D012016:Reference Values; D012709:Serum Albumin / Q000032:analysis; D013536:Suture Techniques; D013997:Time Factors; D016896:Treatment Outcome; D014508:Urea / Q000097:blood; D014945:Wound Healing
D012709:Serum Albumin; D014508:Urea; D002784:Cholesterol; D001663:Bilirubin