Remote ischemic preconditioning and tacrolimus in the fetal small bowel transplant in mice.
To evaluate the effect of remote ischemic preconditioning (IPC-R) in the fetal small bowel transplantation model.
Two groups were constituted: The Isogenic transplant (ISO, C57BL/6 mice, n=24) and the allogenic transplant (ALO, BALB/c mice, n=24). In each group, the animals were distributed with and without IPC-R. It was obtained the following subgroups: Tx, IPC-R, Fk, IPC-Fk, in both strains. Intestinal grafts were stained with hematoxylin and eosin and immunohistochemically.
The graft development evaluation in ISO group showed that IPC-R reduced the development compared with ISO-Tx (5.2±0.4 vs 9.0±0.8) and IPC-R-Fk increased the graft development compared with IPC-R (11.2±0.7 and 10.2±0.8). In ALO group, IPC-Fk increased the development compared with ALO-Tx and ALO with IPC-R (6.0±0.8, 9.0±1.2, 0.0±0.0, 0.5±0.3). The PCNA expression was increased in ISO group treated with Fk and IPC-R compared to other groups (12.2±0.8 vs Tx: 8.8±0.9, IPC-R: 8.0±0.4 and Fk: 9.0±0.6). The graft rejection was lower in groups treated with IPC-R (-18%), Fk (-68%) or both (-61%) compared with ALO-Tx.
Remote ischemic preconditioning showed benefic effect even associate with Tacrolimus on the development and acute rejection of the fetal small bowel graft in the Isogenic and Allogenic transplants.
PhD, Operative Technique and Experimental Surgery Division, Medical School, Universidade Federal de São Paulo (UNIFESP), Brazil. Technical procedures, acquisition of data, manuscript writing.
Ricardo José
RJ
Morello
PhD, Laboratory of Emergency Medicine - LIM-51, Faculty of Medicine (FM), Universidade de São Paulo (USP), Brazil. Interpretation of data, histopathological examinations, statistical analysis, manuscript writing.
Marcia Kiyomi
MK
Koike
PhD, Operative Technique and Experimental Surgery Division, Medical School, Universidade Federal de São Paulo (UNIFESP), Brazil. Technical procedures, acquisition of data, manuscript writing.
Marcos de Souza
MS
Abrahão
Associate Professor, Medical School, Universidade Federal do Vale do São Francisco (UNIVASF), Brazil. Manuscript writing, critical revision.
Karen Ruggeri
KR
Saad
Associate Professor, Medical School, Universidade Federal do Vale do São Francisco (UNIVASF), Brazil. Manuscript writing, critical revision.
Paulo Fernandes
PF
Saad
Associate Professor, Surgical Physiopathology Laboratory (LIM-62), FMUSP. Affiliate Professor, Operative Technique and Experimental Surgery Division, Medical School, UNIFESP, Sao Paulo-SP, Brazil. Conception, intellectual and scientific content of the study; critical revision; final approval.
Edna Frasson de Souza
EF
Montero
D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects; D005260:Female; D016332:Fetal Tissue Transplantation / Q000379:methods*; D006084:Graft Rejection / Q000517:prevention & control; D007150:Immunohistochemistry; D007166:Immunosuppressive Agents / Q000627:therapeutic use*; D007421:Intestine, Small / Q000098:blood supply* / Q000637:transplantation*; D019194:Ischemic Preconditioning / Q000379:methods*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D015203:Reproducibility of Results; D016559:Tacrolimus / Q000627:therapeutic use*; D013997:Time Factors; D014185:Transplantation, Isogeneic; D016896:Treatment Outcome
D007166:Immunosuppressive Agents; D016559:Tacrolimus