Deep Digging: Far Red Imaging for the Monitoring of Transplanted Hepatocytes in Rats.
Technologies for in vivo imaging of the distribution and integration of cell transplants gain significance for the use of novel cell therapy approaches in regenerative medicine. Applied to adequate animal models, they provide information on the spatio-temporal engraftment and functional performance of the cells transplanted. This chapter includes a detailed description of the in vivo tracking of transplanted hepatocytes in rat liver including the conjugation of antibodies to fluorochromes for far red imaging using a multispectral optical imager.
Clinics and Policlinics of Visceral-, Transplantation-, Thoracic- and Vascular Surgery, Applied Molecular Hepatology, University of Leipzig, Liebigstraße 21, 04103, Leipzig, Germany. peggy.stock@medizin.uni-leipzig.de.
Peggy
P
Stock
Department of Surgery, University of Leipzig, Leipzig, Germany.
Kristin
K
Weber
D000818:Animals; D058846:Antibodies, Monoclonal, Murine-Derived / Q000008:administration & dosage; D049109:Cell Proliferation / Q000187:drug effects; D058948:Cell Tracking / Q000379:methods*; D017690:Cell Transplantation / Q000379:methods*; D018819:Dipeptidyl Peptidase 4 / Q000235:genetics / Q000276:immunology; D005456:Fluorescent Dyes / Q000008:administration & dosage; D006498:Hepatectomy / Q000379:methods; D022781:Hepatocytes / Q000187:drug effects / Q000637:transplantation*; D007274:Injections, Intraperitoneal; D007275:Injections, Intravenous; D008099:Liver / Q000098:blood supply / Q000166:cytology / Q000000981:diagnostic imaging* / Q000601:surgery; D062048:Narrow Band Imaging / Q000379:methods*; D010477:Perfusion / Q000379:methods; D011169:Portal Vein / Q000601:surgery; D011763:Pyrrolizidine Alkaloids / Q000633:toxicity; D051381:Rats; D011916:Rats, Inbred F344; D019172:Transplantation Conditioning / Q000379:methods*
D058846:Antibodies, Monoclonal, Murine-Derived; D005456:Fluorescent Dyes; D011763:Pyrrolizidine Alkaloids; C000602659:DPP4 protein, rat; D018819:Dipeptidyl Peptidase 4; C003300:retrorsine
Cell-based therapy; Far red technology; Hepatocyte transplantation; In vivo imaging