Rutin inhibits proliferation, attenuates superoxide production and decreases adhesion and migration of human cancerous cells.
Lung and colorectal cancer are the principal causes of death in the world. Rutin, an active flavonoid compound, is known for possessing a wide range of biological activities. In this study, we examined the effect of rutin on the viability, superoxide anion production, adhesion and migration of human lung (A549) and colon (HT29 and Caco-2) cancer cell lines. In order to control the harmlessness of the tested concentrations of rutin, the viability of cancer cell lines was assessed using a 3-(4,5-dimethylthiazol- 2-yl)-2,5-diphenyl-tetrazolium bromide (MTT) assay. ROS generation was measured by lucigenin chemiluminescence detecting superoxide ions. To investigate the effect of rutin on the behavior of human lung and colon cancer cell lines, we performed adhesion assays, using various purified extracellular matrix (ECM) proteins. Finally, in vitro cell migration assays were explored using modified Boyden chambers. The viability of cancerous cells was inhibited by rutin. It also significantly attenuated the superoxide production in HT29 cells. In addition, rutin affected adhesion and migration of A549 and HT29 cell. These findings indicate that rutin, a natural molecule, might have potential as anticancer agent against lung and colorectal carcinogenesis.
Laboratory for Forest Ecology, National Institute for Research in Rural Engineering, Water and Forests, University of carthage, BP 10, 2080 Ariana, Tunisia. Electronic address: medbensghaier@gmail.com.
Mohamed
M
Ben Sghaier
Centre de Recherche en Oncologie Biologique et Oncopharmacologie (CRO2), INSERM UMR 911, Faculté de Pharmacie, Marseille, France; Aix-Marseille Université, France.
Alessandra
A
Pagano
Centre de Recherche en Oncologie Biologique et Oncopharmacologie (CRO2), INSERM UMR 911, Faculté de Pharmacie, Marseille, France; Aix-Marseille Université, France.
Mohamed
M
Mousslim
Laboratory for Forest Ecology, National Institute for Research in Rural Engineering, Water and Forests, University of carthage, BP 10, 2080 Ariana, Tunisia.
Youssef
Y
Ammari
Centre de Recherche en Oncologie Biologique et Oncopharmacologie (CRO2), INSERM UMR 911, Faculté de Pharmacie, Marseille, France; Aix-Marseille Université, France.
Hervé
H
Kovacic
Centre de Recherche en Oncologie Biologique et Oncopharmacologie (CRO2), INSERM UMR 911, Faculté de Pharmacie, Marseille, France; Aix-Marseille Université, France.
José
J
Luis
D000072283:A549 Cells; D018938:Caco-2 Cells; D002448:Cell Adhesion / Q000187:drug effects* / Q000502:physiology; D002465:Cell Movement / Q000187:drug effects* / Q000502:physiology; D049109:Cell Proliferation / Q000187:drug effects* / Q000502:physiology; D002470:Cell Survival / Q000187:drug effects / Q000502:physiology; D004305:Dose-Response Relationship, Drug; D019073:HT29 Cells; D006801:Humans; D017382:Reactive Oxygen Species / Q000037:antagonists & inhibitors* / Q000378:metabolism; D012431:Rutin / Q000494:pharmacology*; D013481:Superoxides / Q000037:antagonists & inhibitors* / Q000378:metabolism
D017382:Reactive Oxygen Species; D013481:Superoxides; D012431:Rutin
Adhesion; Colorectal cancer; Lung cancer; Migration; Rutin; Superoxide production