Effects of two commercial diets and technical feed treatment on stomach lesions and immune system of fattening pigs.
The impact of technical feed treatment and diet on stomach lesions and traits of the local and systemic immune system were investigated in fattening pigs. Feeding groups differed in technical feed treatment (standard ground meal vs. finely ground and pelleted feed) and diet (soya bean meal vs. rapeseed meal/DDGS/soya beans). Pigs were fattened approximately 10 weeks by ad libitum feeding and slaughtered subsequently. Gastric alterations were assessed by a macroscopic scoring system [macroscopic stomach score (MSC) 0 =  normal to 4 =  severe lesions]. For immunological investigations, lymphocytes from blood and jejunal tissues were isolated. T-cell phenotyping was carried out by staining intestinal lymphocytes with monoclonal antibodies for CD4 and CD8 and flow cytometric measurements. MSC was higher in animals fed finely ground and pelleted feed compared with their counterparts. Significant interactions between diet and feed treatment considering the MSC were observed (p = 0.027). There was no effect of diet or technical feed treatment on T cells of blood, Lymphonodi gastrici or lamina propria (LP) and intraepithelial cells. However, technical feed treatment significantly affected subsets of CD4+, CD8+, CD8low, CD4/CD8 double-positive T cells, the mean fluorescence intensity of CD4+T cells and the ratio of CD8low/CD8highT cells in Peyer's patches (PP). All named parameters were reduced in PP of animals fed finely ground and pelleted feed compared with animals fed standard ground meal. Furthermore, significant differences between T cells of lymph nodes and LP were observed between animals with middle MSC (MSC = 1-2.5) and animals with high MSC (MSC = 3-4). Significant alterations in T cells of PP were observed between animals of low (MSC = 0-0.5) and high MSC. The observed effects provide the evidence that the impact of technical feed treatment is not limited on the stomach lesions. Possible stimuli and consequences of the immune system should be studied in more detail.
Institute of Animal Nutrition, Friedrich-Loeffler-Institut, Federal Institute for Animal Health, Brunswick, Germany.
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W
Liermann
Institute of Animal Nutrition, Friedrich-Loeffler-Institut, Federal Institute for Animal Health, Brunswick, Germany.
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A
Berk
Institute of Animal Nutrition, Friedrich-Loeffler-Institut, Federal Institute for Animal Health, Brunswick, Germany.
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J
Frahm
Research Institute of Feed Technology of the International Research Association of Feed Technology e.V., Brunswick, Germany.
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V
Böschen
Institute of Animal Nutrition, Friedrich-Loeffler-Institut, Federal Institute for Animal Health, Brunswick, Germany.
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S
Dänicke
D056704:Adaptive Immunity; D000821:Animal Feed; D000818:Animals; D004032:Diet / Q000662:veterinary*; D005511:Food Handling; D005766:Gastrointestinal Contents; D008297:Male; D013270:Stomach / Q000276:immunology; D013272:Stomach Diseases / Q000473:pathology / Q000517:prevention & control / Q000662:veterinary*; D013552:Swine / Q000276:immunology* / Q000502:physiology*; D013553:Swine Diseases / Q000517:prevention & control*; D016176:T-Lymphocyte Subsets / Q000502:physiology
CD4+ cells; CD8+ cells; intestinal immune system; pelleting; pigs; stomach