Diminished effector and memory CD8+ circulating T lymphocytes in patients with severe influenza caused by the AH1N1 pdm09 virus.
The T cell immune response to viral infection includes the expansion of naïve T cells, effector cell differentiation and the induction of long-lived memory cells. We compared the differentiation of CD8+T cells in patients with severe or mild pneumonia induced by influenza infection occurring during the 2009 influenza outbreak and compared their T cell subsets with those in blood samples obtained from healthy volunteers before the AH1N1 influenza outbreak in Mexico. Patients with severe influenza exhibited significantly lower numbers of effector memory CD8+CD26highCD45RO+CCR7+phenotype and lower numbers of central memory CD8+CD26highCD62L+CCR7+, CD26highCD62L+CD127+or CD26highCD45RO+CD57lowphenotypes than patients with mild influenza or unexposed healthy subjects. Effector T cells with CD8+CD26CD62LlowCD57+phenotype were significantly diminished in severe influenza patients compared to those in patients with mild influenza or unexposed healthy subjects. These results suggest that low levels of circulating CD8+T effector and central memory cells are associated with influenza severity.
Departamento de Microbiología, Instituto Nacional de Enfermedades Respiratorias Ismael Cosío Villegas, Mexico City 14080, Mexico. Electronic address: yolag@hotmail.com.
Yolanda
Y
Gonzalez
Departamento de Microbiología, Instituto Nacional de Enfermedades Respiratorias Ismael Cosío Villegas, Mexico City 14080, Mexico. Electronic address: ejuarez@iner.gob.mx.
Esmeralda
E
Juárez
Departamento de Microbiología, Instituto Nacional de Enfermedades Respiratorias Ismael Cosío Villegas, Mexico City 14080, Mexico. Electronic address: carranza.salazar.claudia@gmail.com.
Claudia
C
Carranza
Departamento de Microbiología, Instituto Nacional de Enfermedades Respiratorias Ismael Cosío Villegas, Mexico City 14080, Mexico. Electronic address: eduardosadadiaz@yahoo.com.
Eduardo
E
Sada
Unidad de Bioquímica, Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán, Mexico City 14000, Mexico. Electronic address: sigifredo.pedraza9@gmail.com.
Sigifredo
S
Pedraza-Sánchez
Departamento de Microbiología, Instituto Nacional de Enfermedades Respiratorias Ismael Cosío Villegas, Mexico City 14080, Mexico. Electronic address: marthatorres98@yahoo.com.
Martha
M
Torres
D018414:CD8-Positive T-Lymphocytes / Q000276:immunology* / Q000821:virology; D006801:Humans; D007156:Immunologic Memory; D053118:Influenza A Virus, H1N1 Subtype / Q000235:genetics / Q000276:immunology* / Q000502:physiology; D007251:Influenza, Human / Q000276:immunology* / Q000821:virology; D008800:Mexico; D016176:T-Lymphocyte Subsets / Q000276:immunology / Q000821:virology
H1N1pdm09 virus; Influenza severity; Memory CD8+ T lymphocytes