The Abscission Checkpoint: Making It to the Final Cut.
Cytokinesis is the final stage of cell division and is concluded by abscission of the intercellular bridge to physically separate the daughter cells. Timing of cytokinetic abscission is monitored by a molecular machinery termed the abscission checkpoint. This machinery delays abscission in cells with persistent chromatin in the intercellular bridge. Recent work has also uncovered its response to high membrane tension, nuclear pore defects, and DNA replication stress. Although it is known that the abscission checkpoint depends on persistent activity of the Aurora B protein kinase, we have only recently begun to understand its molecular basis. We propose here a molecular framework for abscission checkpoint signaling and we discuss outstanding questions relating to its function and physiological relevance.
Centre for Cancer Biomedicine, Faculty of Medicine, University of Oslo, Montebello, 0379 Oslo, Norway; Department of Molecular Cell Biology, Institute for Cancer Research, Oslo University Hospital, Montebello, 0379 Oslo, Norway.
Viola
V
Nähse
Centre for Cancer Biomedicine, Faculty of Medicine, University of Oslo, Montebello, 0379 Oslo, Norway; Department of Molecular Cell Biology, Institute for Cancer Research, Oslo University Hospital, Montebello, 0379 Oslo, Norway.
Liliane
L
Christ
Centre for Cancer Biomedicine, Faculty of Medicine, University of Oslo, Montebello, 0379 Oslo, Norway; Department of Molecular Cell Biology, Institute for Cancer Research, Oslo University Hospital, Montebello, 0379 Oslo, Norway; Centre of Molecular Inflammation Research, Norwegian University of Science and Technology, Faculty of Medicine, 7491 Trondheim, Norway. Electronic address: stenmark@ulrik.uio.no.
Harald
H
Stenmark
Centre for Cancer Biomedicine, Faculty of Medicine, University of Oslo, Montebello, 0379 Oslo, Norway; Department of Molecular Cell Biology, Institute for Cancer Research, Oslo University Hospital, Montebello, 0379 Oslo, Norway. Electronic address: coen.campsteijn@rr-research.no.
Coen
C
Campsteijn
D000818:Animals; D059447:Cell Cycle Checkpoints; D048749:Cytokinesis; D004261:DNA Replication; D056827:Endosomal Sorting Complexes Required for Transport / Q000378:metabolism; D006801:Humans; D008954:Models, Biological; D015398:Signal Transduction
D056827:Endosomal Sorting Complexes Required for Transport
Aurora B; ESCRT-III; NoCut; chromatin bridge; cytokinesis; genome instability; membrane scission