The Analysis of CD83 Expression on Human Immune Cells Identifies a Unique CD83+-Activated T Cell Population.
CD83 is a member of the Ig gene superfamily, first identified in activated lymphocytes. Since then, CD83 has become an important marker for defining activated human dendritic cells (DC). Several potential CD83 mRNA isoforms have been described, including a soluble form detected in human serum, which may have an immunosuppressive function. To further understand the biology of CD83, we examined its expression in different human immune cell types before and after activation using a panel of mouse and human anti-human CD83 mAb. The mouse anti-human CD83 mAbs, HB15a and HB15e, and the human anti-human CD83 mAb, 3C12C, were selected to examine cytoplasmic and surface CD83 expression, based on their different binding characteristics. Glycosylation of CD83, the CD83 mRNA isoforms, and soluble CD83 released differed among blood DC, monocytes, and monocyte-derived DC, and other immune cell types. A small T cell population expressing surface CD83 was identified upon T cell stimulation and during allogeneic MLR. This subpopulation appeared specifically during viral Ag challenge. We did not observe human CD83 on unstimulated human natural regulatory T cells (Treg), in contrast to reports describing expression of CD83 on mouse Treg. CD83 expression was increased on CD4+, CD8+T, and Treg cells in association with clinical acute graft-versus-host disease in allogeneic hematopoietic cell transplant recipients. The differential expression and function of CD83 on human immune cells reveal potential new roles for this molecule as a target of therapeutic manipulation in transplantation, inflammation, and autoimmune diseases.
ANZAC Research Institute, Concord Repatriation General Hospital, Sydney, New South Wales 2139, Australia.
Xinsheng
0000-0001-9755-9908
X
Ju
ANZAC Research Institute, Concord Repatriation General Hospital, Sydney, New South Wales 2139, Australia.|Sydney Medical School, University of Sydney, Sydney, New South Wales 2006, Australia.
Pablo A
0000-0001-5074-8824
PA
Silveira
ANZAC Research Institute, Concord Repatriation General Hospital, Sydney, New South Wales 2139, Australia.|Sydney Medical School, University of Sydney, Sydney, New South Wales 2006, Australia.
Wei-Hsun
0000-0002-6902-4719
WH
Hsu
ANZAC Research Institute, Concord Repatriation General Hospital, Sydney, New South Wales 2139, Australia.
Zehra
Z
Elgundi
ANZAC Research Institute, Concord Repatriation General Hospital, Sydney, New South Wales 2139, Australia.
Renz
0000-0002-4456-9368
R
Alingcastre
ANZAC Research Institute, Concord Repatriation General Hospital, Sydney, New South Wales 2139, Australia.
Nirupama D
ND
Verma
ANZAC Research Institute, Concord Repatriation General Hospital, Sydney, New South Wales 2139, Australia.|Sydney Medical School, University of Sydney, Sydney, New South Wales 2006, Australia.
Phillip D
0000-0001-7047-1113
PD
Fromm
ANZAC Research Institute, Concord Repatriation General Hospital, Sydney, New South Wales 2139, Australia.|Institute of Haematology, Royal Prince Alfred Hospital, University of Sydney, Sydney, New South Wales 2050, Australia.
Jennifer L
0000-0001-9920-0720
JL
Hsu
ANZAC Research Institute, Concord Repatriation General Hospital, Sydney, New South Wales 2139, Australia.|Institute of Haematology, Royal Prince Alfred Hospital, University of Sydney, Sydney, New South Wales 2050, Australia.
Christian
C
Bryant
ANZAC Research Institute, Concord Repatriation General Hospital, Sydney, New South Wales 2139, Australia.|Sydney Medical School, University of Sydney, Sydney, New South Wales 2006, Australia.
Ziduo
0000-0002-4173-6394
Z
Li
ANZAC Research Institute, Concord Repatriation General Hospital, Sydney, New South Wales 2139, Australia.
Fiona
F
Kupresanin
ANZAC Research Institute, Concord Repatriation General Hospital, Sydney, New South Wales 2139, Australia.|Sydney Medical School, University of Sydney, Sydney, New South Wales 2006, Australia.
Tsun-Ho
0000-0003-0229-6004
TH
Lo
Anatomical Pathology Department, Concord Repatriation General Hospital, Sydney, New South Wales 2139, Australia; and.
Candice
C
Clarke
Sydney Medical School, University of Sydney, Sydney, New South Wales 2006, Australia.|Anatomical Pathology Department, Concord Repatriation General Hospital, Sydney, New South Wales 2139, Australia; and.
Kenneth
0000-0001-8700-5581
K
Lee
Centenary Institute, Royal Prince Alfred Hospital, Sydney, New South Wales 2050, Australia.
Helen
0000-0003-2047-6543
H
McGuire
Centenary Institute, Royal Prince Alfred Hospital, Sydney, New South Wales 2050, Australia.
Barbara
0000-0001-6817-9690
B
Fazekas de St Groth
Sydney Medical School, University of Sydney, Sydney, New South Wales 2006, Australia.|Institute of Haematology, Royal Prince Alfred Hospital, University of Sydney, Sydney, New South Wales 2050, Australia.
Stephen R
SR
Larsen
Sydney Medical School, University of Sydney, Sydney, New South Wales 2006, Australia.|Institute of Haematology, Royal Prince Alfred Hospital, University of Sydney, Sydney, New South Wales 2050, Australia.
John
J
Gibson
ANZAC Research Institute, Concord Repatriation General Hospital, Sydney, New South Wales 2139, Australia.|Sydney Medical School, University of Sydney, Sydney, New South Wales 2006, Australia.
Kenneth F
KF
Bradstock
ANZAC Research Institute, Concord Repatriation General Hospital, Sydney, New South Wales 2139, Australia.|Sydney Medical School, University of Sydney, Sydney, New South Wales 2006, Australia.
Georgina J
0000-0001-7894-232X
GJ
Clark
ANZAC Research Institute, Concord Repatriation General Hospital, Sydney, New South Wales 2139, Australia; derek.hart@sydney.edu.au.|Sydney Medical School, University of Sydney, Sydney, New South Wales 2006, Australia.
Derek N J
0000-0003-2620-1612
DN
Hart
D000208:Acute Disease; D000818:Animals; D015703:Antigens, CD / Q000235:genetics / Q000378:metabolism*; D000956:Antigens, Viral / Q000276:immunology; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000276:immunology*; D006031:Glycosylation; D006086:Graft vs Host Disease / Q000276:immunology*; D018380:Hematopoietic Stem Cell Transplantation; D006801:Humans; D007136:Immunoglobulins / Q000235:genetics / Q000378:metabolism*; D008213:Lymphocyte Activation; D008562:Membrane Glycoproteins / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D009000:Monocytes / Q000276:immunology*; D059368:RNA Isoforms / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics; D016176:T-Lymphocyte Subsets / Q000276:immunology*; D050378:T-Lymphocytes, Regulatory / Q000276:immunology*; D014184:Transplantation, Homologous; D000097802:CD83 Antigen
D015703:Antigens, CD; D000956:Antigens, Viral; D007136:Immunoglobulins; D008562:Membrane Glycoproteins; D059368:RNA Isoforms; D012333:RNA, Messenger; D000097802:CD83 Antigen