omicsNPC: Applying the Non-Parametric Combination Methodology to the Integrative Analysis of Heterogeneous Omics Data.
BACKGROUND
The advance of omics technologies has made possible to measure several data modalities on a system of interest. In this work, we illustrate how the Non-Parametric Combination methodology, namely NPC, can be used for simultaneously assessing the association of different molecular quantities with an outcome of interest. We argue that NPC methods have several potential applications in integrating heterogeneous omics technologies, as for example identifying genes whose methylation and transcriptional levels are jointly deregulated, or finding proteins whose abundance shows the same trends of the expression of their encoding genes.


RESULTS
We implemented the NPC methodology within "omicsNPC", an R function specifically tailored for the characteristics of omics data. We compare omicsNPC against a range of alternative methods on simulated as well as on real data. Comparisons on simulated data point out that omicsNPC produces unbiased / calibrated p-values and performs equally or significantly better than the other methods included in the study; furthermore, the analysis of real data show that omicsNPC (a) exhibits higher statistical power than other methods, (b) it is easily applicable in a number of different scenarios, and (c) its results have improved biological interpretability.


CONCLUSIONS
The omicsNPC function competitively behaves in all comparisons conducted in this study. Taking into account that the method (i) requires minimal assumptions, (ii) it can be used on different studies designs and (iii) it captures the dependences among heterogeneous data modalities, omicsNPC provides a flexible and statistically powerful solution for the integrative analysis of different omics data.
Institute of Computer Science, Foundation for Research and Technology-Hellas, Heraklion, Greece.
Nestoras
N
Karathanasis
Department of Computer Science, University of Crete, Heraklion, Greece.
Ioannis
I
Tsamardinos
Department of Computer Science, University of Crete, Heraklion, Greece.
Vincenzo
http://orcid.org/0000-0002-6552-6076
V
Lagani
D001943:Breast Neoplasms / Q000235:genetics / Q000473:pathology; D020869:Gene Expression Profiling; D023281:Genomics; D005909:Glioblastoma / Q000235:genetics; D006801:Humans; D009361:Neoplasm Invasiveness; D012559:Schizophrenia / Q000235:genetics; D013223:Statistics as Topic / Q000379:methods*; D018709:Statistics, Nonparametric