Filamentous Bacteria as Targets to Study Phagocytosis.
Filamentous targets are internalized via phagocytic cups that last for several minutes before closing to form a phagosome. This characteristic offers the possibility to study key events in phagocytosis with greater spatial and temporal resolution than is possible to achieve using spherical particles, for which the transition from a phagocytic cup to an enclosed phagosome occurs within a few seconds after particle attachment. In this chapter, we provide methodologies to prepare filamentous bacteria and describe how they can be used as targets to study different aspects of phagocytosis.
Department of Biological Sciences, University of Toronto Scarborough, 1265 Military Trail, Toronto, ON, Canada, M1C 1A4.
Akriti
A
Prashar
Department of Biological Sciences, University of Toronto Scarborough, 1265 Military Trail, Toronto, ON, Canada, M1C 1A4.
Sana I S
SI
Khan
Department of Biological Sciences, University of Toronto Scarborough, 1265 Military Trail, Toronto, ON, Canada, M1C 1A4. terebiznik@utsc.utoronto.ca.
Mauricio R
MR
Terebiznik
D000818:Animals; D001419:Bacteria / Q000378:metabolism*; D001681:Biological Assay / Q000379:methods*; D008264:Macrophages / Q000382:microbiology; D051379:Mice; D010587:Phagocytosis; D010588:Phagosomes / Q000378:metabolism; D000067996:RAW 264.7 Cells
Filamentous bacteria; Phagocytic cup formation; Phagocytosis; Phagosome maturation; Target morphology