Knockdown of DIXDC1 Inhibits the Proliferation and Migration of Human Glioma Cells.
DIX domain containing 1 (DIXDC1), the human homolog of coiled-coil-DIX1 (Ccd1), is a positive regulator of Wnt signaling pathway. Recently, it was found to act as a candidate oncogene in colon cancer, non-small-cell lung cancer, and gastric cancer. In this study, we aimed to investigate the clinical significance of DIXDC1 expression in human glioma and its biological function in glioma cells. Western blot and immunohistochemistry analysis showed that DIXDC1 was overexpressed in glioma tissues and glioma cell lines. The expression level of DIXDC1 was evidently linked to glioma pathological grade and Ki-67 expression. Kaplan-Meier curve showed that high expression of DIXDC1 may lead to poor outcome of glioma patients. Serum starvation and refeeding assay indicated that the expression of DIXDC1 was associated with cell cycle. To determine whether DIXDC1 could regulate the proliferation and migration of glioma cells, we transfected glioma cells with interfering RNA-targeting DIXDC1; investigated cell proliferation with Cell Counting Kit (CCK)-8, flow cytometry assays, and colony formation analyses; and investigated cell migration with wound healing assays and transwell assays. According to our data, knockdown of DIXDC1 significantly inhibited proliferation and migration of glioma cells. These data implied that DIXDC1 might participate in the development of glioma, suggesting that DIXDC1 can become a potential therapeutic strategy for glioma.
Department of Neurosurgery, Affiliated Hospital of Nantong University, Xisi Road No. 20, Nantong, 226001, Jiangsu, People's Republic of China.
Jianguo
J
Chen
Department of Radiotherapy and Oncology, Affiliated Hospital of Nantong University, Xisi Road No. 20, Nantong, 226001, Jiangsu, People's Republic of China.
Chaoyan
C
Shen
Department of Neurosurgery, Affiliated Hospital of Nantong University, Xisi Road No. 20, Nantong, 226001, Jiangsu, People's Republic of China.
Jinlong
J
Shi
Department of Neurosurgery, Affiliated Hospital of Nantong University, Xisi Road No. 20, Nantong, 226001, Jiangsu, People's Republic of China.
Jianhong
J
Shen
Department of Radiotherapy and Oncology, Affiliated Hospital of Nantong University, Xisi Road No. 20, Nantong, 226001, Jiangsu, People's Republic of China.
Wenjuan
W
Chen
Department of Radiotherapy and Oncology, Affiliated Hospital of Nantong University, Xisi Road No. 20, Nantong, 226001, Jiangsu, People's Republic of China.
Jie
J
Sun
Department of Neurosurgery, Affiliated Hospital of Nantong University, Xisi Road No. 20, Nantong, 226001, Jiangsu, People's Republic of China.
Shaocheng
S
Fan
Department of Radiotherapy and Oncology, Affiliated Hospital of Nantong University, Xisi Road No. 20, Nantong, 226001, Jiangsu, People's Republic of China.
Yuanqi
Y
Bei
Jiangsu Province Key Laboratory for Inflammation and Molecular Drug Target, Medical College of Nantong University, Nantong, 226001, Jiangsu, People's Republic of China.
Peng
P
Xu
Department of Neurosurgery, Wuxi Second Hospital Affiliated to Nanjing Medical University, Wuxi, 214002, Jiangsu, People's Republic of China.
Hao
H
Chang
Department of Neurosurgery, Affiliated Hospital of Nantong University, Xisi Road No. 20, Nantong, 226001, Jiangsu, People's Republic of China.
Rui
R
Jiang
Department of Radiotherapy and Oncology, Affiliated Hospital of Nantong University, Xisi Road No. 20, Nantong, 226001, Jiangsu, People's Republic of China.
Lu
L
Hua
Department of Radiotherapy and Oncology, Affiliated Hospital of Nantong University, Xisi Road No. 20, Nantong, 226001, Jiangsu, People's Republic of China. jibinrobert@163.com.
Bin
B
Ji
Department of Neurosurgery, Affiliated Hospital of Nantong University, Xisi Road No. 20, Nantong, 226001, Jiangsu, People's Republic of China. hqf025@ntu.edu.cn.
Qingfeng
Q
Huang
D000328:Adult; D001932:Brain Neoplasms / Q000473:pathology*; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D015536:Down-Regulation; D005260:Female; D055785:Gene Knockdown Techniques; D005910:Glioma / Q000473:pathology*; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins / Q000378:metabolism*; D053208:Kaplan-Meier Estimate; D008297:Male; D008840:Microfilament Proteins / Q000378:metabolism*; D008875:Middle Aged; D016016:Proportional Hazards Models; D015854:Up-Regulation
C513335:DIXDC1 protein, human; D047908:Intracellular Signaling Peptides and Proteins; D008840:Microfilament Proteins
DIXDC1; Glioma; Migration; Prognosis; Proliferation