Differential proteomics analysis of Frankliniella occidentalis immune response after infection with Tomato spotted wilt virus (Tospovirus).
Tomato spotted wilt virus (TSWV) is mainly vectored by Frankliniella occidentalis Pergande, and it potentially activates the vector's immune response. However, molecular background of the altered immune response is not clearly understood. Therefore, using a proteomic approach, we investigated the immune pathways that are activated in F. occidentalis larvae after 24 h exposure to TSWV. Two-dimensional isoelectric focusing/sodium dodecyl sulfate polyacrylamide gel electrophoresis (2D-IEF/SDS/PAGE) combined with mass spectrometry (MS), were used to identify proteins that were differentially expressed upon viral infection. High numbers of proteins were abundantly expressed in F. occidentalis exposed to TSWV (73%) compared to the non-exposed (27%), with the majority functionally linked to the innate immune system such as: signaling, stress response, defense response, translation, cellular lipids and nucleotide metabolism. Key proteins included: 70 kDa heat shock proteins, Ubiquitin and Dermcidin, among others, indicative of a responsive pattern of the vector's innate immune system to viral infection.
Department of Phytomedicine, Institute of Horticultural Production Systems, Gottfried Wilhelm Leibniz Universität Hannover, Herrenhäuser Strasse 2, 30419 Hannover, Germany. Electronic address: ogada@ipp.uni-hannover.de.
Pamella Akoth
PA
Ogada
Department of Plant Molecular Biology, Institute of Plant Genetics, Gottfried Wilhelm Leibniz Universität Hannover, Herrenhäuser Strasse 2, 30419 Hannover, Germany.
Leonard Muriithi
LM
Kiirika
Leibniz-Institut für Analytische Wissenschaften - ISAS - e.V., Otto-Hahn-Straße 6b, 44227 Dortmund, Germany; Department of Plant Proteomics, Institute of Plant Genetics, Gottfried Wilhelm Leibniz Universität Hannover, Herrenhäuser Strasse 2, 30419 Hannover, Germany.
Christin
C
Lorenz
Department of Plant Proteomics, Institute of Plant Genetics, Gottfried Wilhelm Leibniz Universität Hannover, Herrenhäuser Strasse 2, 30419 Hannover, Germany.
Jennifer
J
Senkler
Department of Plant Proteomics, Institute of Plant Genetics, Gottfried Wilhelm Leibniz Universität Hannover, Herrenhäuser Strasse 2, 30419 Hannover, Germany.
Hans-Peter
HP
Braun
Department of Phytomedicine, Institute of Horticultural Production Systems, Gottfried Wilhelm Leibniz Universität Hannover, Herrenhäuser Strasse 2, 30419 Hannover, Germany.
Hans-Michael
HM
Poehling
D000818:Animals; D001179:Arthropod Vectors / Q000276:immunology*; D005786:Gene Expression Regulation; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism; D007113:Immunity, Innate / Q000235:genetics; D019476:Insect Proteins / Q000235:genetics / Q000378:metabolism; D007814:Larva; D050356:Lipid Metabolism / Q000235:genetics; D013058:Mass Spectrometry; D010455:Peptides / Q000235:genetics / Q000378:metabolism; D040901:Proteomics; D061149:Thysanoptera / Q000276:immunology*; D017865:Tospovirus / Q000276:immunology*; D059467:Transcriptome; D025801:Ubiquitin / Q000235:genetics / Q000378:metabolism
D018840:HSP70 Heat-Shock Proteins; D019476:Insect Proteins; D010455:Peptides; D025801:Ubiquitin; C442243:dermcidin
2D-IEF/SDS/PAGE; Frankliniella occidentalis; Innate immunity; Mass spectrometry; Proteomics; Tomato spotted wilt virus