Histopathological and Immunohistochemical Analysis of Small Intestinal Biopsies in Adults Suspected of Celiac Disease.
OBJECTIVE
To analyse histomorphological and immunohistochemical analysis of small intestinal biopsies in adults suspected of celiac disease.


METHODS
Descriptive study.


METHODS
Department of Histopathology, Army Medical College, Rawalpindi, from November 2014 to December 2015.


METHODS
Fifty cases of small intestinal mucosal biopsies (duodenal and jejunal) were analysed in adult patients aged above 14 years suspected of celiac disease. Their histomorphological data was recorded using Modified Marsh Criteria. Type of intraepithelial lymphocytes was assessed using immunohistochemistry. Intraepithelial lymphocytes were counted both by H&E stain and immunostain CD3 and CD20.


RESULTS
Thirty-four percent patients were aged between 21 - 30 years and 22% patients aged between 41 - 50 years. There were 84% (n=42) males. Thirteen (26%) cases showed focal villous atrophy, 32 (64%) cases showed partial villous atrophy and 5 (10%) cases showed complete villous atrophy. Anti-tissue transglutaminase antibody was positive in 21 (42%) cases. CD3 immunomarker was positive for intraepithelial lymphocytes in all 50 cases while CD20 immunomarker showed focal positivity in areas with lymphoid follicle formation. The count of intraepithelial lymphocytes was found to be almost equal (with a difference of 3 - 4 lymphocytes) on both H&E stain and immunostain CD3 and CD20.


CONCLUSIONS
Males aged 21 - 30 years were the most commonly affected group. The most frequent change in histology was partial villous atrophy along with lymphocytic enteritis. All the intraepithelial lymphocytes were present in crescendo pattern of distribution.
Department of Pathology, Army Medical College, Rawalpindi.
Rida
R
Iftikhar
Department of Pathology, Army Medical College, Rawalpindi.
Shahid
S
Jamal
Department of Pathology, Army Medical College, Rawalpindi.
Ambreen
A
Zafar
Department of Pathology, Army Medical College, Rawalpindi.
Aiza
A
Saadia
D000293:Adolescent; D000328:Adult; D017677:Age Distribution; D000368:Aged; D001284:Atrophy / Q000473:pathology; D015415:Biomarkers / Q000032:analysis; D001706:Biopsy; D017252:CD3 Complex / Q000032:analysis; D016827:CD8 Antigens / Q000032:analysis; D002446:Celiac Disease / Q000097:blood / Q000150:complications / Q000175:diagnosis / Q000276:immunology* / Q000473:pathology*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D007413:Intestinal Mucosa / Q000276:immunology* / Q000473:pathology*; D007421:Intestine, Small / Q000276:immunology* / Q000473:pathology*; D018655:Lymphocyte Count; D008214:Lymphocytes / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D055815:Young Adult
D015415:Biomarkers; D017252:CD3 Complex; D016827:CD8 Antigens