Engineering deacetoxycephalosporin C synthase as a catalyst for the bioconversion of penicillins.
7-aminodeacetoxycephalosporanic acid (7-ADCA) is a key intermediate of many clinically useful semisynthetic cephalosporins that were traditionally prepared by processes involving chemical ring expansion of penicillin G. Bioconversion of penicillins to cephalosporins using deacetoxycephalosporin C synthase (DAOCS) is an alternative and environmentally friendly process for 7-ADCA production. Arnold Demain and co-workers pioneered such a process. Later, protein engineering efforts to improve the substrate specificity and catalytic efficiency of DAOCS for penicillins have been made by many groups, and a whole cell process using Escherichia coli for bioconversion of penicillins has been developed.
State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, No. 1 West Beichen Road, Beijing, 100101, People's Republic of China.
Keqiang
K
Fan
ASCR Key Laboratory of Microbial Physiological and Metabolic Engineering, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, People's Republic of China.
Baixue
B
Lin
ASCR Key Laboratory of Microbial Physiological and Metabolic Engineering, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, People's Republic of China.
Yong
Y
Tao
State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, No. 1 West Beichen Road, Beijing, 100101, People's Republic of China. yangkq@im.ac.cn.
Keqian
K
Yang
D055162:Biocatalysis; D002511:Cephalosporins / Q000096:biosynthesis* / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D010400:Penicillin G / Q000378:metabolism; D010406:Penicillins / Q000096:biosynthesis* / Q000378:metabolism; D013379:Substrate Specificity
D002511:Cephalosporins; D010406:Penicillins; C025578:deacetoxycephalosporin C; C014320:7-aminodesacetoxycephalosporanic acid; D010400:Penicillin G
7-aminodeacetoxycephalosporanic acid (7-ADCA); Bioconversion; Deacetoxycephalosporin C synthase (DAOCS); Penicillin G; Penicillin expandase