Effect of Potential Probiotic Lactococcus lactis Subsp. lactis on Growth Performance, Intestinal Microbiota, Digestive Enzyme Activities, and Disease Resistance of Litopenaeus vannamei.
The aims of this study were to evaluate the effects of Lactococcus lactis subsp. lactis on the growth, intestinal microbiota, digestive enzyme activity, and disease resistance of Litopenaeus vannamei. Diets containing four different concentrations of L. lactis (0 [basal diet], 106, 107, and 108CFU g-1) were fed to white shrimps L. vannamei (average weight 5.89 ± 0.36 g) for 8 weeks. At the end of the feeding trial, shrimps were immersed in Caspian Seawater (10.8 ppt) contaminated with 106CFU ml-1pathogenic V. anguillarum for 2 h. Results revealed that growth rate, survival, and body protein level were increased with dietary supplementation of L. lactis. The activities of digestive enzymes (cellulose, lipase, amylase, and protease) were significantly higher in the groups fed with diets containing 107or 108CFU g-1L. lactis than those in the control. The Lactobacillus and Bacillus counts were higher (P < 0.05) in the intestine of shrimps fed with L. lactis-supplemented diets. In addition, higher level of L. lactis supplementation decreased the Vibrio counts. Moreover, L. vannamei fed diet supplemented with 108CFU g-1of L. lactis exhibited significantly the highest hematocyte count and post-challenge survival rate (79.2 %). Collectively, these results suggest that dietary supplementation of L. lactis subsp. lactis at 108CFU g-1can promote growth performance, digestive enzyme activity, and disease resistance of L. vannamei.
Department of Aquatic Animal Health and Diseases, Iranian Fisheries Science Research Institute (IFSRI), Agricultural Research Education and Extension Organization (AREEO), Tehran, Iran.
Milad
M
Adel
Oceanography Department, Faculty of Science, Alexandria University, Alexandria, Egypt.
Abdel-Fattah M
AM
El-Sayed
Department of Fisheries, Sari Agricultural Sciences and Natural Resources University, Sari, Iran.
Sakineh
S
Yeganeh
Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran.
Maryam
M
Dadar
Laboratory of Aquatic Biomedicine, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Seoul, South Korea. gribiotek@gmail.com.
Sib Sankar
0000-0002-2383-1937
SS
Giri
D000681:Amylases / Q000378:metabolism; D000821:Animal Feed / Q000032:analysis; D000818:Animals; D060467:Disease Resistance; D000069196:Gastrointestinal Microbiome / Q000187:drug effects*; D007422:Intestines / Q000201:enzymology / Q000276:immunology / Q000382:microbiology; D013294:Lactococcus lactis / Q000502:physiology*; D008049:Lipase / Q000378:metabolism; D033561:Penaeidae / Q000201:enzymology / Q000254:growth & development* / Q000276:immunology / Q000382:microbiology; D010447:Peptide Hydrolases / Q000378:metabolism; D019936:Probiotics / Q000008:administration & dosage*; D014733:Vibrio / Q000502:physiology
D008049:Lipase; D000681:Amylases; D010447:Peptide Hydrolases
Digestive enzyme; Disease resistance; Growth performance; Intestinal microbiota; Lactococcus lactis subsp. lactis