Routine identification of Nocardia species by MALDI-TOF mass spectrometry.
We here show adequate species identification for bacterial isolates of the genus Nocardia spp. through VITEK mass spectrometry. Application of a specific sample preparation method in combination with a robust matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) database leads to 94% accurate identification to the species level on a set of 164 isolates. The possibility to identify Nocardia spp. using MALDI-TOF MS will be available in the next release of VITEK MS update (IVD Version 3.0).
R&D Microbiology, bioMérieux, La Balme Les Grottes, France. Electronic address: victoria.girard@biomerieux.com.
Victoria
V
Girard
R&D Microbiology, bioMérieux, La Balme Les Grottes, France.
Sandrine
S
Mailler
R&D Microbiology, bioMérieux, La Balme Les Grottes, France.
Sophie
S
Polsinelli
R&D Microbiology, bioMérieux, La Balme Les Grottes, France.
Delphine
D
Jacob
R&D Microbiology, bioMérieux, La Balme Les Grottes, France.
Marie Christine
MC
Saccomani
R&D Microbiology, bioMérieux, La Balme Les Grottes, France.
Beatrice
B
Celliere
R&D Microbiology, bioMérieux, La Balme Les Grottes, France.
Valerie
V
Monnin
R&D Microbiology, bioMérieux, La Balme Les Grottes, France.
Alex
A
van Belkum
Canisius-Wilhelmina Hospital, Department of Medical Microbiology and Infectious Diseases, Nijmegen, The Netherlands.
Ferry
F
Hagen
Canisius-Wilhelmina Hospital, Department of Medical Microbiology and Infectious Diseases, Nijmegen, The Netherlands; Department of Medical Microbiology, Radboud University Medical Center, Nijmegen, The Netherlands.
Jacques F
JF
Meis
R&D Microbiology, bioMérieux, La Balme Les Grottes, France.
Géraldine
G
Durand
D001431:Bacteriological Techniques / Q000379:methods*; D006801:Humans; D009615:Nocardia / Q000737:chemistry* / Q000145:classification*; D009617:Nocardia Infections / Q000175:diagnosis*; D013048:Specimen Handling / Q000379:methods; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
MALDI TOF MS; Nocardia; Routine diagnostic microbiology; Species identification