Detection of MicroRNAs in Brain Slices Using In Situ Hybridization.
MicroRNAs are key posttranscriptional regulators of protein levels in cells. The brain is particularly enriched in microRNAs, and important roles have been demonstrated for these noncoding RNAs in various neurological disorders. To this end, visualization of microRNAs in specific cell types and subcellular compartments within tissue sections provides researchers with essential insights that support understanding of the cell and molecular mechanisms of microRNAs in brain diseases. In this chapter we describe an in situ hybridization protocol for the detection of microRNAs in mouse brain sections, which provides cellular resolution of the expression of microRNAs in the brain.
Department of Physiology and Medical Physics, Royal College of Surgeons in Ireland, Dublin, Ireland.
Sean
S
Quinlan
Department of Physiology and Medical Physics, Royal College of Surgeons in Ireland, Dublin, Ireland.
Christine
C
Henke
Department of Physiology and Medical Physics, Royal College of Surgeons in Ireland, Dublin, Ireland.
Gary P
GP
Brennan
Department of Physiology and Medical Physics, Royal College of Surgeons in Ireland, Dublin, Ireland.
David C
DC
Henshall
Department of Physiology and Medical Physics, Royal College of Surgeons in Ireland, Dublin, Ireland. evajimenez@rcsi.ie.
Eva M
EM
Jimenez-Mateos
D000818:Animals; D001921:Brain / Q000378:metabolism*; D017403:In Situ Hybridization; D051379:Mice; D035683:MicroRNAs / Q000378:metabolism*; D016707:Tissue Fixation
D035683:MicroRNAs
Brain; CNS; In situ hybridization; Neurodegenerative disorders; Staining; microRNA