Association of oncogenic bacteria with colorectal cancer in South China.
To quantify Fusobacterium spp., Enterococcus faecalis (E.faecalis), Enterotoxigenic Bacteroides fragilis (ETBF), and Enteropathogenic Escherichia coli in colorectal cancer (CRC) patients and their possible association with CRC clinicopathogical features, we collected the resected tumors and adjacent normal tissues (N) from 97 CRC patients. 48 age- and sex-matched healthy controls (HC) were also recruited. Real-time PCR was used for bacterial quantification. The median abundance ofFusobacterium spp.(p < 0.001, vs. N; p < 0.01,vs. HC), E.faecalis (p < 0.05, vs. N; p < 0.01, vs. HC) and ETBF (p < 0.001, vs. N; p < 0.05,vs. HC) in tumor tissues was significantly higher than that detected in normal tissue and HC. E.faecalis was detected in 95.88% of tumors and 93.81% of adjacent tissues. Fusobacterium spp. was detected in 72.16% of tumors and 67.01% of adjacent tissues. The combined E.faecalis and Fusobacterium spp. were detected in 70.10% of tumors and 36.08% of adjacent normal tissues. All four bacteria were detected in 33.72% and 22.09% of paired tumor and adjacent normal tissues, respectively. E.faecalis and Fusobacterium spp. are enriched in both tumor and adjacent tissue of CRC patients when compared to HC, suggesting that it is possible to be previously undetected changes in the pathohistologically normal colon tissue in the proximity of the tumor.
Department of Gastroenterology, Guangzhou Digestive Disease Center, Guangzhou First People's Hospital, Guangzhou Medical University, Guangzhou, 510180, China.
Youlian
Y
Zhou
The First People's Foshan Hospital, Chancheng District, Foshan, 528000, Guangdong, China.
Hanchang
H
He
Department of Gastroenterology, Guangzhou Digestive Disease Center, Guangzhou First People's Hospital, Guangzhou Medical University, Guangzhou, 510180, China.
Haoming
H
Xu
Department of Gastroenterology, Guangzhou Digestive Disease Center, Guangzhou First People's Hospital, Guangzhou Medical University, Guangzhou, 510180, China.
Yingfei
Y
Li
Department of Gastroenterology, Guangzhou Digestive Disease Center, Guangzhou First People's Hospital, Guangzhou Medical University, Guangzhou, 510180, China.
Zhiming
Z
Li
Department of Gastroenterology, Guangzhou Digestive Disease Center, Guangzhou First People's Hospital, Guangzhou Medical University, Guangzhou, 510180, China.
Yanlei
Y
Du
Department of Gastroenterology, Guangzhou Digestive Disease Center, Guangzhou First People's Hospital, Guangzhou Medical University, Guangzhou, 510180, China.
Jie
J
He
Department of Gastroenterology, Guangzhou Digestive Disease Center, Guangzhou First People's Hospital, Guangzhou Medical University, Guangzhou, 510180, China.
Yongjian
Y
Zhou
Department of Gastroenterology, Guangzhou Digestive Disease Center, Guangzhou First People's Hospital, Guangzhou Medical University, Guangzhou, 510180, China.
Hong
H
Wang
Department of Gastroenterology, Guangzhou Digestive Disease Center, Guangzhou First People's Hospital, Guangzhou Medical University, Guangzhou, 510180, China.
Yuqiang
Y
Nie
D000230:Adenocarcinoma / Q000175:diagnosis / Q000453:epidemiology / Q000382:microbiology*; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001441:Bacteroides fragilis / Q000235:genetics / Q000302:isolation & purification*; D016022:Case-Control Studies; D002471:Cell Transformation, Neoplastic; D002681:China / Q000453:epidemiology; D003106:Colon / Q000382:microbiology*; D015179:Colorectal Neoplasms / Q000175:diagnosis / Q000453:epidemiology / Q000382:microbiology*; D004269:DNA, Bacterial / Q000235:genetics; D013293:Enterococcus faecalis / Q000235:genetics / Q000302:isolation & purification*; D054308:Enteropathogenic Escherichia coli / Q000235:genetics / Q000302:isolation & purification*; D005260:Female; D005673:Fusobacterium / Q000235:genetics / Q000302:isolation & purification*; D000069196:Gastrointestinal Microbiome / Q000235:genetics; D054884:Host-Pathogen Interactions; D006801:Humans; D008297:Male; D008875:Middle Aged; D060888:Real-Time Polymerase Chain Reaction
D004269:DNA, Bacterial
colorectal cancer; enterococcus faecalis; enteropathogenic escherichia coli; enterotoxigenic bacteroidesfragilis; fusobacterium spp.