One-step in situ solid-substrate-based whole blood immunoassay based on FRET between upconversion and gold nanoparticles.
Despite their general clinical applications, current fluorescence-based immunoassays are confronted with serious challenges, e.g. the advance serum/ plasma separation and the tedious washing process in current heterogeneous approaches, and aggregation of particles, low sensitivity and the narrow linear range in homogeneous approaches. In this paper, these urgent problems were solved in a novel one-step in situ immunoassay of whole blood samples by combining the traditional fluorescence resonance energy transfer (FRET) technology (between upconversion nanoparticles (UCNPs) and gold nanoparticles (GNPs)) and the solid-substrate based immunoassay technology. The low detection limits of goat IgG (gIgG) as 0.042μg/mL in buffers, 0.51μg/mL in 20-fold diluted whole blood samples and a wide linear range from 0.75μg/mL to 60μg/mL in blood samples were achieved. To the best of our knowledge, it is the first one-step in situ solid-substrate-based immunoassay of whole blood samples with large linear detection range. This development provides a promising platform for a rapid and sensitive immunoassay of various bio-molecules directly in whole blood without tedious separation, washing steps and aggregation problems.
State Key Laboratory of Luminescence and Applications, Changchun Institute of Optics, Fine Mechanics and Physics, Chinese Academy of Sciences, Changchun 130033, China; Graduate University of the Chinese Academy of Sciences, Beijing 100049, China.
Cuixia
C
Li
State Key Laboratory of Luminescence and Applications, Changchun Institute of Optics, Fine Mechanics and Physics, Chinese Academy of Sciences, Changchun 130033, China; Graduate University of the Chinese Academy of Sciences, Beijing 100049, China; Van't Hoff Institute for Molecular Sciences, University of Amsterdam, Science Park 904, 1098 XH Amsterdam, The Netherlands.
Jing
J
Zuo
State Key Laboratory of Luminescence and Applications, Changchun Institute of Optics, Fine Mechanics and Physics, Chinese Academy of Sciences, Changchun 130033, China; Graduate University of the Chinese Academy of Sciences, Beijing 100049, China.
Qiqing
Q
Li
State Key Laboratory of Luminescence and Applications, Changchun Institute of Optics, Fine Mechanics and Physics, Chinese Academy of Sciences, Changchun 130033, China.
Yulei
Y
Chang
State Key Laboratory of Luminescence and Applications, Changchun Institute of Optics, Fine Mechanics and Physics, Chinese Academy of Sciences, Changchun 130033, China. Electronic address: zhangyl0109@sina.com.
Youlin
Y
Zhang
State Key Laboratory of Luminescence and Applications, Changchun Institute of Optics, Fine Mechanics and Physics, Chinese Academy of Sciences, Changchun 130033, China.
Langping
L
Tu
State Key Laboratory of Luminescence and Applications, Changchun Institute of Optics, Fine Mechanics and Physics, Chinese Academy of Sciences, Changchun 130033, China.
Xiaomin
X
Liu
State Key Laboratory of Luminescence and Applications, Changchun Institute of Optics, Fine Mechanics and Physics, Chinese Academy of Sciences, Changchun 130033, China.
Bin
B
Xue
Department of Basic Medicine, Gerontology Department of First Bethune Hospital, University of Jilin, Changchun 130021, China.
Huiying
H
Zhao
Van't Hoff Institute for Molecular Sciences, University of Amsterdam, Science Park 904, 1098 XH Amsterdam, The Netherlands. Electronic address: h.zhang@uva.nl.
Hong
H
Zhang
State Key Laboratory of Luminescence and Applications, Changchun Institute of Optics, Fine Mechanics and Physics, Chinese Academy of Sciences, Changchun 130033, China. Electronic address: xgkong14@ciomp.ac.cn.
Xianggui
X
Kong
D000818:Animals; D004871:Erbium / Q000737:chemistry*; D031541:Fluorescence Resonance Energy Transfer / Q000379:methods*; D005455:Fluorescent Antibody Technique / Q000379:methods; D005459:Fluorides / Q000737:chemistry*; D006041:Goats; D006046:Gold / Q000737:chemistry*; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis / Q000097:blood*; D057230:Limit of Detection; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D011817:Rabbits; D015018:Ytterbium / Q000737:chemistry*; D015019:Yttrium / Q000737:chemistry*
D007074:Immunoglobulin G; C525362:sodium yttriumtetrafluoride; D015019:Yttrium; D006046:Gold; D004871:Erbium; D015018:Ytterbium; D005459:Fluorides
FRET; Immunoassay; In situ; Upconversion nanoparticles; Whole blood