Combination of OipA, BabA, and SabA as candidate biomarkers for predicting Helicobacter pylori-related gastric cancer.
Helicobacter pylori (H. pylori ) infection is a major cause of chronic gastritis and is highly related to duodenal ulcer (DU) and gastric cancer (GC). To identify H. pylori-related GC biomarkers with high seropositivity in GC patients, differences in levels of protein expression between H. pylori from GC and DU patients were analyzed by isobaric tag for relative and absolute quantitation (iTRAQ). In total, 99 proteins showed increased expression (>1.5-fold) in GC patients compared to DU patients, and 40 of these proteins were categorized by KEGG pathway. The four human disease-related adhesin identified, AlpA, OipA, BabA, and SabA, were potential GC-related antigens, with a higher seropositivity in GC patients (n = 76) than in non-GC patients (n = 100). Discrimination between GC and non-GC patients was improved using multiple antigens, with an odds ratio of 9.16 (95% CI, 2.99-28.07; p < 0.0001) for three antigens recognized. The optimized combination of OipA, BabA, and SabA gave a 77.3% correct prediction rate. A GC-related protein microarray was further developed using these antigens. The combination of OipA, BabA, and SabA showed significant improvement in the diagnostic accuracy and the protein microarray containing above antigens should provide a rapid and convenient diagnosis of H. pylori-associated GC.
Graduate Institute of Biochemistry and Molecular Biology, College of Medicine, National Taiwan University, Taipei, Taiwan.
Yu-Lin
YL
Su
Graduate Institute of Biochemistry and Molecular Biology, College of Medicine, National Taiwan University, Taipei, Taiwan.
Hsiang-Ling
HL
Huang
Graduate Institute of Biochemistry and Molecular Biology, College of Medicine, National Taiwan University, Taipei, Taiwan.
Bo-Shih
BS
Huang
Graduate Institute of Systems Biology and Bioinformatics, National Central University, Taoyuan, Taiwan.
Po-Chung
PC
Chen
Graduate Institute of Systems Biology and Bioinformatics, National Central University, Taoyuan, Taiwan.
Chien-Sheng
CS
Chen
Department of Statistics, National Taipei University, New Taipei City, Taiwan.
Hong-Long
HL
Wang
Graduate Institute of Biochemistry and Molecular Biology, College of Medicine, National Taiwan University, Taipei, Taiwan.
Pin-Hsin
PH
Lin
First Core Laboratory, College of Medicine, National Taiwan University, Taipei, Taiwan.
Meng-Shu
MS
Chieh
Department of Medical Laboratory Science and Biotechnology, Center of Infectious Disease and Signaling Research, College of Medicine, National Cheng Kung University, Tainan, Taiwan.
Jiunn-Jong
JJ
Wu
Department of Internal Medicine, Hospital and College of Medicine, National Taiwan University, Taipei, Taiwan.
Jyh-Chin
JC
Yang
Graduate Institute of Biochemistry and Molecular Biology, College of Medicine, National Taiwan University, Taipei, Taiwan.|Center of Genomic Medicine, National Taiwan University, Taipei, Taiwan.
Lu-Ping
LP
Chow
D018829:Adhesins, Bacterial / Q000737:chemistry / Q000276:immunology / Q000378:metabolism*; D000907:Antibodies, Bacterial / Q000097:blood; D000942:Antigens, Bacterial / Q000276:immunology / Q000378:metabolism; D001425:Bacterial Outer Membrane Proteins / Q000737:chemistry / Q000276:immunology / Q000378:metabolism*; D015415:Biomarkers / Q000378:metabolism*; D004381:Duodenal Ulcer / Q000150:complications / Q000382:microbiology / Q000473:pathology; D016481:Helicobacter Infections / Q000150:complications / Q000382:microbiology / Q000473:pathology*; D016480:Helicobacter pylori / Q000302:isolation & purification / Q000378:metabolism*; D006801:Humans; D007118:Immunoassay; D016017:Odds Ratio; D010455:Peptides / Q000032:analysis; D040081:Protein Array Analysis; D040901:Proteomics; D013274:Stomach Neoplasms / Q000175:diagnosis* / Q000209:etiology / Q000473:pathology; D015854:Up-Regulation
D018829:Adhesins, Bacterial; D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; C400429:BabA protein, Helicobacter pylori; D001425:Bacterial Outer Membrane Proteins; D015415:Biomarkers; C526444:OipA protein, Helicobacter pylori; D010455:Peptides; C500808:SabA protein, Helicobacter pylori