Ex Vivo Expansion of Hematopoietic Stem Cells to Improve Engraftment in Stem Cell Transplantation.
The efficient use of hematopoietic stem cells (HSC) for transplantation is often limited by the relatively low numbers of HSC collected. The ex vivo expansion of HSC for clinical use is a potentially valuable and safe approach to increase HSC numbers thereby increasing engraftment and reducing the risk of morbidity from infection. Here, we describe a protocol for the robust ex vivo expansion of human CD34(+) HSC isolated from umbilical cord blood. The protocol described can efficiently generate large numbers of HSC. We also describe a flow cytometry-based method using high-resolution division tracking to characterize the kinetics of HSC growth and differentiation. Utilizing the guidelines discussed, it is possible for investigators to use this protocol as presented or to modify it for their specific needs.
Sydney Cord and Marrow Transplant Facility, Sydney Children's Hospital, Graduate School of Biomedical Engineering, University of New South Wales, High Street, Randwick, NSW, 2031, Australia.
Kap-Hyoun
KH
Ko
Graduate School of Biomedical Engineering, University of New South Wales, Kensington, NSW, Australia.
Robert
R
Nordon
Sydney Cord and Marrow Transplant Facility, Sydney Children's Hospital, Centre for Children's Cancer and Blood Disorders, Sydney Children's Hospital, Randwick, NSW, Australia.
Tracey A
TA
O'Brien
Faculty of Medicine, University of New South Wales, Sydney, NSW, Australia.
Geoff
G
Symonds
Sydney Cord and Marrow Transplant Facility, Sydney Children's Hospital, Graduate School of Biomedical Engineering, University of New South Wales, High Street, Randwick, NSW, 2031, Australia. Alla.Dolnikov@health.nsw.gov.au.|Faculty of Medicine, University of New South Wales, Sydney, NSW, Australia. Alla.Dolnikov@health.nsw.gov.au.
Alla
A
Dolnikov
D000818:Animals; D018952:Antigens, CD34 / Q000378:metabolism; D018929:Cell Culture Techniques / Q000379:methods*; D002454:Cell Differentiation / Q000235:genetics / Q000502:physiology; D005312:Fetal Blood / Q000166:cytology; D005434:Flow Cytometry; D018380:Hematopoietic Stem Cell Transplantation / Q000379:methods*; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000378:metabolism / Q000502:physiology; D006801:Humans
D018952:Antigens, CD34
Cell division; Expansion; Hematopoietic stem cell