Nickel chloride-induced apoptosis via mitochondria- and Fas-mediated caspase-dependent pathways in broiler chickens.
Ni, a metal with industrial and commercial uses, poses a serious hazard to human and animal health. In the present study, we used flow cytometry, immunohistochemistry and qRT-PCR to investigate the mechanisms of NiCl2-induced apoptosis in kidney cells. After treating 280 broiler chickens with 0, 300, 600 or 900 mg/kg NiCl2 for 42 days, we found that two caspase-dependent pathways were involved in the induced renal tubular cell apoptosis. In the mitochondria-mediated caspase-dependent apoptotic pathway, cyt-c, HtrA2/Omi, Smac/Diablo, apaf-1, PARP, and caspase-9, 3, 6 and 7 were all increased, while. XIAP transcription was decreased. Concurrently, in the Fas-mediated caspase-dependent apoptotic pathway, Fas, FasL, caspase-8, caspase-10 and Bid levels were all increased. These results indicate that dietary NiCl2 at 300+ mg/kg induces renal tubular cell apoptosis in broiler chickens, involving both mitochondrial and Fas-mediated caspase-dependent apoptotic pathways. Our results provide novel insight into Ni and Ni-compound toxicology evaluated in vitro and in vivo.
College of Veterinary Medicine, Sichuan Agricultural University, Ya'an 625014, China.
Hongrui
H
Guo
College of Veterinary Medicine, Sichuan Agricultural University, Ya'an 625014, China.|Key Laboratory of Animal Diseases and Environmental Hazards of Sichuan Province, Sichuan Agricultural University, Ya'an 625014, China.
Hengmin
H
Cui
College of Veterinary Medicine, Sichuan Agricultural University, Ya'an 625014, China.|Key Laboratory of Animal Diseases and Environmental Hazards of Sichuan Province, Sichuan Agricultural University, Ya'an 625014, China.
Jing
J
Fang
College of Veterinary Medicine, Sichuan Agricultural University, Ya'an 625014, China.|Key Laboratory of Animal Diseases and Environmental Hazards of Sichuan Province, Sichuan Agricultural University, Ya'an 625014, China.
Zhicai
Z
Zuo
College of Veterinary Medicine, Sichuan Agricultural University, Ya'an 625014, China.|Key Laboratory of Animal Diseases and Environmental Hazards of Sichuan Province, Sichuan Agricultural University, Ya'an 625014, China.
Junliang
J
Deng
College of Veterinary Medicine, Sichuan Agricultural University, Ya'an 625014, China.|Key Laboratory of Animal Diseases and Environmental Hazards of Sichuan Province, Sichuan Agricultural University, Ya'an 625014, China.
Xun
X
Wang
College of Veterinary Medicine, Sichuan Agricultural University, Ya'an 625014, China.|Key Laboratory of Animal Diseases and Environmental Hazards of Sichuan Province, Sichuan Agricultural University, Ya'an 625014, China.
Ling
L
Zhao
College of Veterinary Medicine, Sichuan Agricultural University, Ya'an 625014, China.
Bangyuan
B
Wu
College of Veterinary Medicine, Sichuan Agricultural University, Ya'an 625014, China.
Kejie
K
Chen
College of Veterinary Medicine, Sichuan Agricultural University, Ya'an 625014, China.
Jie
J
Deng
D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D020169:Caspases / Q000378:metabolism*; D002645:Chickens; D004305:Dose-Response Relationship, Drug; D053222:Fas Ligand Protein / Q000378:metabolism*; D007674:Kidney Diseases / Q000139:chemically induced* / Q000201:enzymology / Q000473:pathology; D007684:Kidney Tubules / Q000187:drug effects* / Q000201:enzymology / Q000473:pathology; D008928:Mitochondria / Q000187:drug effects* / Q000201:enzymology / Q000473:pathology; D009532:Nickel / Q000633:toxicity*; D015398:Signal Transduction / Q000187:drug effects; D013997:Time Factors; D019014:fas Receptor / Q000378:metabolism*
D053222:Fas Ligand Protein; D019014:fas Receptor; C022838:nickel chloride; D009532:Nickel; D020169:Caspases
Fas-mediated caspase-dependent apoptosis; NiCl2; apoptosis; kidney; mitochondria-mediated caspase-dependent apoptosis