Brain quantitative proteomic responses reveal new insight of benzotriazole neurotoxicity in female Chinese rare minnow (Gobiocypris rarus).
Benzotriazole (BT) is a high-production volume chemical which has been ubiquitously detected in aquatic environments. Although adverse effects from acute and chronic exposure to BT have been reported, the neurotoxic effect of BT and the mechanisms of toxicity are not well documented. In this study, adult female Chinese rare minnow (Gobiocypris rarus) were exposed to 0.05, 0.5, and 5mg/L BT for 28days. The brain proteome showed that BT exposure mainly involved in metabolic process, signal transduction, stress response, cytoskeleton, and transport. Pathway analysis revealed that cellular processes affected by BT included cellular respiration, G-protein signal cascades, Ca2+-dependent signaling, cell cycle and apoptosis. Moreover, data on relative mRNA levels demonstrated that genes related to these toxic pathways were also significantly affected by BT. Furthermore, proteins affected by BT such as CKBB, GS, HPCA, VDAC1, and FLOT1A are associated with neurological disorders. Therefore, our finding suggested that BT induced molecular responses in the brain and could provide new insight into BT neurotoxicity in Chinese rare minnow.
Key Laboratory of Drinking Water Science and Technology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China; College of Environment and Resources, Inner Mongolia University, Hohhot 010021, China.
Xuefang
X
Liang
Department of Physiological Sciences and Center for Environmental and Human Toxicology, University of Florida, Gainesville, FL 32611, USA.
Christopher J
CJ
Martyniuk
Key Laboratory of Drinking Water Science and Technology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China; Beijing Key Laboratory of Industrial Wastewater Treatment and Reuse, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China. Electronic address: jmzha@rcees.ac.cn.
Jinmiao
J
Zha
Key Laboratory of Drinking Water Science and Technology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China; State Key Laboratory of Environmental Aquatic Chemistry, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China.
Zijian
Z
Wang
D000818:Animals; D001921:Brain / Q000378:metabolism*; D002681:China; D016000:Cluster Analysis; D003530:Cyprinidae / Q000254:growth & development / Q000378:metabolism*; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D029941:Fish Proteins / Q000235:genetics / Q000378:metabolism; D051597:Hippocalcin / Q000235:genetics / Q000378:metabolism; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism; D020543:Proteome / Q000187:drug effects* / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D014230:Triazoles / Q000633:toxicity*; D050995:Voltage-Dependent Anion Channel 1 / Q000235:genetics / Q000378:metabolism; D014874:Water Pollutants, Chemical / Q000737:chemistry / Q000633:toxicity*
D029941:Fish Proteins; D008565:Membrane Proteins; D020543:Proteome; D012333:RNA, Messenger; D014230:Triazoles; D014874:Water Pollutants, Chemical; C106354:flotillins; D051597:Hippocalcin; C012771:benzotriazole; D050995:Voltage-Dependent Anion Channel 1
Benzotriazole; Brain proteome; Neurotoxicity; Rare minnow (Gobiocypris rarus)