Genome-Wide Analysis of Differentially Expressed microRNA in Bombyx mori Infected with Nucleopolyhedrosis Virus.
Bombyx mori nucleopolyhedrosis virus (BmNPV) is a major pathogen that threatens the growth and sustainability of the sericulture industry. Since microRNAs (miRNAs) have been shown to play important roles in host-pathogen interactions, in this study we investigated the effects of BmNPV infection on silkworm microRNAs expression profile. To achieve this, we constructed and deep-sequenced two small RNA libraries generated from BmNPV infected and un-infected larvae. The results revealed that 38 silkworm miRNAs were differentially expressed after BmNPV infection. Based on the GO analysis, their predicted target genes were found to be involved in diverse functions such as binding, catalytic, virion and immune response to stimulus suggesting their potential roles in host-virus interactions. Using the dual-luciferase reporter assay, we confirmed that Bmo-miR-277-5p, up-regulated in BmNPV-infected larvae, targeted the B. mori DNA cytosine-5 methyltransferase (Dnmt2) gene which may play potential role in silkworm-BmNPV interaction. These results provide new insights into exploring the interaction mechanism between silkworm and BmNPV.
Sericultural Research Institute, Jiangsu University of Science and Technology, Zhenjiang Jiangsu, China.|Quality Inspection Center for Sericulture Products, Ministry of Agriculture, Zhenjiang Jiangsu, China.
Ping
P
Wu
School of Biology, Jiangsu University of Science and Technology, Zhenjiang Jiangsu, China.
Xiaoxu
X
Jiang
Sericultural Research Institute, Jiangsu University of Science and Technology, Zhenjiang Jiangsu, China.
Xijie
X
Guo
Sericultural Research Institute, Jiangsu University of Science and Technology, Zhenjiang Jiangsu, China.|Quality Inspection Center for Sericulture Products, Ministry of Agriculture, Zhenjiang Jiangsu, China.
Long
L
Li
Sericultural Research Institute, Jiangsu University of Science and Technology, Zhenjiang Jiangsu, China.|Quality Inspection Center for Sericulture Products, Ministry of Agriculture, Zhenjiang Jiangsu, China.
Tao
T
Chen
D000818:Animals; D012831:Bombyx / Q000235:genetics / Q000821:virology*; D004248:DNA (Cytosine-5-)-Methyltransferases / Q000235:genetics; D005786:Gene Expression Regulation; D063990:Gene Ontology; D015698:Genomic Library; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods*; D054884:Host-Pathogen Interactions; D035683:MicroRNAs / Q000235:genetics*; D017924:Nucleopolyhedroviruses / Q000201:enzymology / Q000502:physiology*; D017423:Sequence Analysis, RNA / Q000379:methods*; D014764:Viral Proteins / Q000235:genetics
D035683:MicroRNAs; D014764:Viral Proteins; D004248:DNA (Cytosine-5-)-Methyltransferases