Comparison of Regulation Mechanisms of Five Mulberry Ingredients on Insulin Secretion under Oxidative Stress.
The effects of mulberry ingredients including 1-deoxynojrimycin (DNJ), resveratrol (RES), oxyresveratrol (OXY), cyanidin-3-glucoside (C3G), and cyanidin-3-rutinoside (C3R) on insulin secretion under oxidative stress were investigated. The results revealed that they had distinct effects on insulin secretion in H2O2-induced MIN 6 cells, especially DNJ, C3G, and C3R, while RES and OXY showed modest effects in low dose (12.5 μM). The mechanisms were demonstrated in signal pathway that after treatment with DNJ, C3G, and C3R, the expressions of glucokinase (GK) were up-regulated, leading to intracellular ATP accumulation and insulin secretion. They also bound to glucagon-like peptide-1 receptor (GLP-1R), improved GLP-1R, duodenal homeobox factor-1 (PDX-1) expression, and stimulated insulin secretion. Moreover, ROS production was inhibited, followed by a decreasing apoptosis rate, while RES and OXY accelerated the apoptosis at high dose (50 μM). This work expounded the potential mechanisms of mulberry ingredients on insulin secretion, indicating the potential application in the intervention against hyperglycemia.
State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology , 130 Meilong Road, Shanghai 200237, People's Republic of China.|Shanghai Collaborative Innovation Center for Biomanufacturing Technology , 130 Meilong Road, Shanghai 200237, People's Republic of China.
Yun-Chong
YC
Zheng
State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology , 130 Meilong Road, Shanghai 200237, People's Republic of China.|Shanghai Collaborative Innovation Center for Biomanufacturing Technology , 130 Meilong Road, Shanghai 200237, People's Republic of China.
Hao
H
He
State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology , 130 Meilong Road, Shanghai 200237, People's Republic of China.|Shanghai Collaborative Innovation Center for Biomanufacturing Technology , 130 Meilong Road, Shanghai 200237, People's Republic of China.
Xing
X
Wei
Clinical Nutrition Department, Shanghai Jiaotong University Affiliated Sixth People's Hospital , Shanghai 200233, People's Republic of China.
Sheng
S
Ge
State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology , 130 Meilong Road, Shanghai 200237, People's Republic of China.|Shanghai Collaborative Innovation Center for Biomanufacturing Technology , 130 Meilong Road, Shanghai 200237, People's Republic of China.
Yan-Hua
YH
Lu
D000818:Animals; D000872:Anthocyanins / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects; D002460:Cell Line; D005941:Glucokinase / Q000235:genetics / Q000378:metabolism; D005960:Glucosides / Q000494:pharmacology; D018398:Homeodomain Proteins / Q000235:genetics / Q000378:metabolism; D007328:Insulin / Q000378:metabolism*; D050417:Insulin-Secreting Cells / Q000187:drug effects / Q000378:metabolism; D051379:Mice; D031625:Morus / Q000737:chemistry*; D018384:Oxidative Stress / Q000187:drug effects*; D017239:Paclitaxel / Q000031:analogs & derivatives / Q000494:pharmacology; D010936:Plant Extracts / Q000494:pharmacology*; D000077185:Resveratrol; D015398:Signal Transduction; D013267:Stilbenes / Q000494:pharmacology; D015534:Trans-Activators / Q000235:genetics / Q000378:metabolism
C496493:1-deoxypaclitaxel; D000872:Anthocyanins; D005960:Glucosides; D018398:Homeodomain Proteins; D007328:Insulin; D010936:Plant Extracts; D013267:Stilbenes; D015534:Trans-Activators; C462279:cyanidin-3-O-beta-glucopyranoside; C084010:pancreatic and duodenal homeobox 1 protein; C034912:puag-haad; D005941:Glucokinase; C428983:cyanidin 3-rutinoside; D017239:Paclitaxel; D000077185:Resveratrol
insulin secretion; mulberry; oxidative stress; β-cells apoptosis