The effect of hippophae rhamnoides extract on oral mucositis induced in rats with methotrexate.
To investigate the effect of HRE (Hippophae rhamnoides extract) on oral mucositis induced in rats with MTX.
Experimental animals were divided into groups as healthy (HG), HRE+MTX (HMTX), and control group, which received MTX (MTXC). HMTX group received 50 mg/kg HRE while MTXC and HG groups received equivolume distilled water with gavage once a day. After one hour of HRE and distilled water administration, HMTX and MTXC groups received a single dose of oral MTX 5 mg/ kg. This procedure was repeated for one month.
The levels of MDA, IL-1β, and TNF-α were found to be significantly higher in the cheek, lower lip, and tongue tissue of the animals receiving MTX, compared with HG and HMTX groups; however, these parameters were lower in the cheek and low lip tissue, and a milder damage ocurred in these tissues, compared with the tongue tissue in MTXC group. No histopathologic damage was observed in the cheek, lower lip, and tongue tissues of the rats treated with HRE.
This findings indicate that HRE as a natural product is an important advantage compared with synthetic drugs for prophylaxis of oral mucositis developed due to MTX.
Erzurum Education and Research Hospital, Ear Nose and Throat Head&Neck Surgery Clinic, Erzurum, Turkey.
Ozan
O
Kuduban
Hinis State Hospital, Department of Otorhinolaryngology, Erzurum, Turkey.
Muhammed Recai
MR
Mazlumoglu
Palandoken State Hospital, Department of Plastic Reconstructive and Esthetic Surgery, Erzurum, Turkey.
Selma Denktas
SD
Kuduban
Erzincan University, Faculty of Medicine, Department of Otorhinolaryngology, Erzincan, Turkey.
Ertugrul
E
Erhan
Erzincan University, Faculty of Medicine, Department of Pharmacology, Erzincan, Turkey.
Nihal
N
Cetin
Ondokuzmayis University, Faculty of Medicine, Department of Pharmacology, Samsun, Turkey.
Osman
O
Kukula
Erzurum Training and Research Hospital, Department of Medical Genetics, Erzurum, Turkey.
Oguzhan
O
Yarali
Mengucek Gazi Education and Research Hospital, Department of Pathology, Erzincan, Turkey.
Ferda Keskin
FK
Cimen
Erzincan University, Faculty of Arts and Sciences, Department of Biology, Erzincan, Turkey.
Murat
M
Cankaya
D000818:Animals; D001808:Blood Vessels / Q000473:pathology; D002610:Cheek / Q000473:pathology; D005493:Folic Acid Antagonists / Q000009:adverse effects*; D015870:Gene Expression; D031281:Hippophae / Q000737:chemistry*; D053583:Interleukin-1beta / Q000032:analysis / Q000187:drug effects; D008046:Lip / Q000473:pathology; D008315:Malondialdehyde / Q000032:analysis; D008727:Methotrexate / Q000009:adverse effects*; D010936:Plant Extracts / Q000494:pharmacology* / Q000627:therapeutic use; D051381:Rats; D015203:Reproducibility of Results; D013280:Stomatitis / Q000139:chemically induced* / Q000188:drug therapy* / Q000473:pathology; D014059:Tongue / Q000473:pathology; D016896:Treatment Outcome; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis / Q000187:drug effects
D005493:Folic Acid Antagonists; D053583:Interleukin-1beta; D010936:Plant Extracts; D014409:Tumor Necrosis Factor-alpha; D008315:Malondialdehyde; D008727:Methotrexate