Lipid vesicular nanocarrier: Quick encapsulation efficiency determination and transcutaneous application.
Nanoscale delivery systems have been widely investigated to overcome the penetration barrier of stratum corneum for effective transcutaneous application. The aim of this study is the development of effective vesicular formulations of ovalbumin and saponin which are able to promote penetration through the skin layers. Three kinds of vesicular formulations have been investigated as carriers, including liposomes, transfersomes and ethosomes, in which cholesterol and/or cationic lipid stearylamine are incorporated. The impact of membrane composition variations on the protein entrapment has been evaluated for each vesicle type. Formulations were characterized for particle size, polydispersity and encapsulation efficiency. The best formulations for each type of vesicle were subjected to in vivo transdermal immunization in mice. Among the three kinds of vesicular carrier, ethosomal nano carrier not only showed the best stability over a two months' storage, but also enabled the highest increase in the titer of serum antibody. In this regard, cationic nano-ethosomes can be considered as a promising vesicular carrier for transdermal vaccines. Meanwhile, we have developed a simple method to determine encapsulation efficiency of vesicular systems, which has potential application as a high throughput screening for vesicular formulations.
Department of Pharmaceutics, School of Pharmacy, Jiangsu University, Zhenjiang, Jiangsu 212013, China. Electronic address: zhangyb@ujs.edu.cn.
Yibang
Y
Zhang
School of Material Science and Engineering, Nanyang Technological University, 50 Nanyang Avenue, 639798, Singapore.
Weibeng
W
Ng
Department of Pharmaceutics, School of Pharmacy, Jiangsu University, Zhenjiang, Jiangsu 212013, China.
Xue
X
Feng
Department of Pharmaceutics, School of Pharmacy, Jiangsu University, Zhenjiang, Jiangsu 212013, China.
Fangying
F
Cao
Department of Immunology, School of Medicine, Jiangsu University, Zhenjiang, Jiangsu 212013, China.
Huaxi
H
Xu
D000279:Administration, Cutaneous; D000588:Amines / Q000737:chemistry; D000818:Animals; D002626:Chemistry, Pharmaceutical / Q000379:methods; D002784:Cholesterol / Q000737:chemistry; D004337:Drug Carriers / Q000737:chemistry; D016503:Drug Delivery Systems; D004355:Drug Stability; D004356:Drug Storage; D005260:Female; D008055:Lipids / Q000737:chemistry; D008081:Liposomes; D051379:Mice; D053758:Nanoparticles; D010047:Ovalbumin / Q000008:administration & dosage* / Q000493:pharmacokinetics / Q000494:pharmacology; D010316:Particle Size; D012503:Saponins / Q000008:administration & dosage* / Q000493:pharmacokinetics / Q000494:pharmacology; D012867:Skin / Q000378:metabolism; D012869:Skin Absorption
D000588:Amines; D004337:Drug Carriers; D008055:Lipids; D008081:Liposomes; D012503:Saponins; D010047:Ovalbumin; D002784:Cholesterol; C009317:stearylamine
Encapsulation efficiency; Ethosome; Liposome; Transdermal immunization; Transfersome