L-arginine as dietary supplement for improving microvascular function.
BACKGROUND
Reduced availability of nitric oxide leads to dysfunction of endothelium which plays an important role in the development of cardiovascular diseases.


OBJECTIVE
The aim of the present study was to determine whether the dietary supplement L-arginine improves the endothelial function of microvessels by increasing nitric oxide production.


METHODS
We undertook experiments on 51 healthy male volunteers, divided into 4 groups based on their age and physical activity since regular physical activity itself increases endothelium-dependent vasodilation. The skin laser Doppler flux was measured in the microvessels before and after the ingestion of L-arginine (0.9 g). The endothelium-dependent vasodilation was assessed by acetylcholine iontophoresis and the endothelium-independent vasodilation by sodium nitroprusside iontophoresis. In addition, we measured endothelium-dependent and endothelium-independent vasodilation in 81 healthy subjects divided into four age groups.


RESULTS
After the ingestion of L-arginine, the endothelium-dependent vasodilation in the young trained subjects increased (paired t-test, p < 0.05), while in the other groups it remained the same. There were no differences in the endothelium-independent vasodilation after ingestion of L-arginine. With aging endothelium-independent vasodilation decreased while endothelium-dependent vasodilation remained mainly unchanged.


CONCLUSIONS
Obtained results demonstrated that a single dose of L-arginine influences endothelium-dependent vasodilation predominantly in young, trained individuals.
Ziva
Z
Melik
Polona
P
Zaletel
Tina
T
Virtic
Ksenija
K
Cankar
D000328:Adult; D000368:Aged; D001120:Arginine / Q000008:administration & dosage / Q000627:therapeutic use*; D019587:Dietary Supplements / Q000032:analysis*; D004730:Endothelium, Vascular / Q000503:physiopathology*; D006801:Humans; D008297:Male; D008875:Middle Aged; D009569:Nitric Oxide / Q000378:metabolism*; D012867:Skin / Q000098:blood supply*; D055815:Young Adult
D009569:Nitric Oxide; D001120:Arginine
L-arginine; aging; endothelium-dependent vasodilation; endothelium-independent vasodilation; laser Doppler flux; nitric oxide; physical activity; skin microcirculation