Aflatoxin B1Detoxification byAspergillus oryzaefrom Meju, a Traditional Korean Fermented Soybean Starter.
Aflatoxins are classified as Group 1 (carcinogenic to humans) by the International Agency for Research on Cancer. In this study, a total of 134 fungal strains were isolated from 65 meju samples, and two fungal isolates were selected as potential aflatoxin B1(AFB1)-biodetoxification fungi. These fungi were identified asAspergillus oryzaeMAO103 andA. oryzaeMAO104 by sequencing the beta-tubulin gene. The twoA. oryzaestrains were able to degrade more than 90% of AFB1(initial concentration: 40 μg/l) in a culture broth in 14 days. The mutagenic effects of AFB1treated withA. oryzaeMAO103 and MAO104 significantly decreased to 5.7% and 6.4%, respectively, in the frame-shift mutation of Ames tests usingSalmonella typhimuriumTA98. The base-substituting mutagenicity of AFB1was also decreased by the two fungi. Moreover, AFB1production byAspergillus flavuswas significantly decreased by the twoA. oryzaestrains on soybean-based agar plates. Our data suggest that the two AFB1-detoxifyingA. oryzaestrains have potential application to control AFB1in foods and feeds.
Department of Integrated Biomedical and Life Science, Korea University, Seoul 02841, Republic of Korea.
Kyu Ri
KR
Lee
Department of Integrated Biomedical and Life Science, Korea University, Seoul 02841, Republic of Korea.
Sun Min
SM
Yang
Department of Integrated Biomedical and Life Science, Korea University, Seoul 02841, Republic of Korea.
Sung Min
SM
Cho
Department of Food Science and Technology, Yeungnam University, Gyeongsan 38541, Republic of Korea.
Myunghee
M
Kim
Department of Integrated Biomedical and Life Science, Korea University, Seoul 02841, Republic of Korea.
Sung-Yong
SY
Hong
Department of Integrated Biomedical and Life Science, Korea University, Seoul 02841, Republic of Korea.
Soo Hyun
SH
Chung
Aflatoxin B1; biodegradation; detoxification; soybean fermentation starter