16S rRNA Amplicon Sequencing for Epidemiological Surveys of Bacteria in Wildlife.
The human impact on natural habitats is increasing the complexity of human-wildlife interactions and leading to the emergence of infectious diseases worldwide. Highly successful synanthropic wildlife species, such as rodents, will undoubtedly play an increasingly important role in transmitting zoonotic diseases. We investigated the potential for recent developments in 16S rRNA amplicon sequencing to facilitate the multiplexing of the large numbers of samples needed to improve our understanding of the risk of zoonotic disease transmission posed by urban rodents in West Africa. In addition to listing pathogenic bacteria in wild populations, as in other high-throughput sequencing (HTS) studies, our approach can estimate essential parameters for studies of zoonotic risk, such as prevalence and patterns of coinfection within individual hosts. However, the estimation of these parameters requires cleaning of the raw data to mitigate the biases generated by HTS methods. We present here an extensive review of these biases and of their consequences, and we propose a comprehensive trimming strategy for managing these biases. We demonstrated the application of this strategy using 711 commensal rodents, including 208Mus musculus domesticus, 189Rattus rattus, 93Mastomys natalensis, and 221Mastomys erythroleucus, collected from 24 villages in Senegal. Seven major genera of pathogenic bacteria were detected in their spleens:Borrelia,Bartonella,Mycoplasma,Ehrlichia,Rickettsia,Streptobacillus, andOrientia.Mycoplasma,Ehrlichia,Rickettsia,Streptobacillus, andOrientiahave never before been detected in West African rodents. Bacterial prevalence ranged from 0% to 90% of individuals per site, depending on the bacterial taxon, rodent species, and site considered, and 26% of rodents displayed coinfection. The 16S rRNA amplicon sequencing strategy presented here has the advantage over other molecular surveillance tools of dealing with a large spectrum of bacterial pathogens without requiring assumptions about their presence in the samples. This approach is therefore particularly suitable to continuous pathogen surveillance in the context of disease-monitoring programs.IMPORTANCESeveral recent public health crises have shown that the surveillance of zoonotic agents in wildlife is important to prevent pandemic risks. High-throughput sequencing (HTS) technologies are potentially useful for this surveillance, but rigorous experimental processes are required for the use of these effective tools in such epidemiological contexts. In particular, HTS introduces biases into the raw data set that might lead to incorrect interpretations. We describe here a procedure for cleaning data before estimating reliable biological parameters, such as positivity, prevalence, and coinfection, using 16S rRNA amplicon sequencing on an Illumina MiSeq platform. This procedure, applied to 711 rodents collected in West Africa, detected several zoonotic bacterial species, including some at high prevalence, despite their never before having been reported for West Africa. In the future, this approach could be adapted for the monitoring of other microbes such as protists, fungi, and even viruses.
INRA, CBGP, Montferrier sur Lez, France.
Maxime
M
Galan
INRA, CBGP, Montferrier sur Lez, France.
Maria
M
Razzauti
INRA, EpiA, Clermont-Ferrand, France.
Emilie
E
Bard
INRA, Sigenae, France; INRA, GABI, AgroParisTech, Université Paris-Saclay, Jouy-en-Josas, France.
Maria
M
Bernard
IRD, CBGP, Montferrier sur Lez, France.
Carine
C
Brouat
INRA, CBGP, Montferrier sur Lez, France.
Nathalie
N
Charbonnel
INRA, CBGP, Montferrier sur Lez, France.
Alexandre
A
Dehne-Garcia
INRA, CBGP, Montferrier sur Lez, France.
Anne
A
Loiseau
INRA, CBGP, Montferrier sur Lez, France.
Caroline
C
Tatard
INRA, CBGP, Montferrier sur Lez, France.
Lucie
L
Tamisier
INRA, Bipar, Maisons-Alfort, France.
Muriel
M
Vayssier-Taussat
CIRAD, AGAP, Montpellier, France.
Helene
H
Vignes
INRA, CBGP, Montferrier sur Lez, France; INRA, Bipar, Maisons-Alfort, France.
Jean-François
0000-0003-0863-5871
JF
Cosson
West Africa; bacteria; emerging infectious diseases; high-throughput sequencing; metabarcoding; molecular epidemiology; next-generation sequencing; rodents; zoonoses