Simultaneous multi-patch-clamp and extracellular-array recordings: Single neuron reflects network activity.
The increasing number of recording electrodes enhances the capability of capturing the network's cooperative activity, however, using too many monitors might alter the properties of the measured neural network and induce noise. Using a technique that merges simultaneous multi-patch-clamp and multi-electrode array recordings of neural networks in-vitro, we show that the membrane potential of a single neuron is a reliable and super-sensitive probe for monitoring such cooperative activities and their detailed rhythms. Specifically, the membrane potential and the spiking activity of a single neuron are either highly correlated or highly anti-correlated with the time-dependent macroscopic activity of the entire network. This surprising observation also sheds light on the cooperative origin of neuronal burst in cultured networks. Our findings present an alternative flexible approach to the technique based on a massive tiling of networks by large-scale arrays of electrodes to monitor their activity.
Department of Physics, Bar-Ilan University, Ramat-Gan 52900, Israel.|Gonda Interdisciplinary Brain Research Center and the Goodman Faculty of Life Sciences, Bar-Ilan University, Ramat-Gan 52900, Israel.
Roni
R
Vardi
Department of Physics, Bar-Ilan University, Ramat-Gan 52900, Israel.
Amir
A
Goldental
Department of Physics, Bar-Ilan University, Ramat-Gan 52900, Israel.
Shira
S
Sardi
Department of Biochemistry and Molecular Biology, Tel Aviv University, Tel Aviv, Israel.|Sagol School of Neuroscience, Tel Aviv University, Tel Aviv, Israel.
Anton
A
Sheinin
Department of Physics, Bar-Ilan University, Ramat-Gan 52900, Israel.|Gonda Interdisciplinary Brain Research Center and the Goodman Faculty of Life Sciences, Bar-Ilan University, Ramat-Gan 52900, Israel.
Ido
I
Kanter
D000200:Action Potentials; D000818:Animals; D002478:Cells, Cultured; D008564:Membrane Potentials; D009474:Neurons / Q000166:cytology / Q000502:physiology*; D018408:Patch-Clamp Techniques / Q000379:methods*; D051381:Rats; D059010:Single-Cell Analysis / Q000295:instrumentation*