Evaluation of two platelet-rich plasma processing methods and two platelet-activation techniques for use in llamas and alpacas.
OBJECTIVE To evaluate 2 processing methods (commercial kit vs conical tube centrifugation) for preparing platelet rich plasma (PRP) for use in llamas and alpacas. SAMPLES Blood samples (30 mL each) aseptically collected from 6 healthy llamas and 6 healthy alpacas. PROCEDURES PRP was prepared from blood samples by use of a commercial kit and by double-step conical tube centrifugation. A CBC was performed for blood and PRP samples. Platelets in PRP samples were activated by means of a freeze-thaw method with or without 23mM CaCl2, and concentrations of platelet-derived growth factor-BB and transforming growth factor-β1were measured. Values were compared between processing methods and camelid species. RESULTS Blood CBC values for llamas and alpacas were similar. The commercial kit yielded a significantly greater degree of platelet enrichment (mean increase, 8.5 fold vs 2.8 fold) and WBC enrichment (mean increase, 3.7 fold vs 1.9 fold) than did conical tube centrifugation. Llamas had a significantly greater degree of platelet enrichment than alpacas by either processing method. No difference in WBC enrichment was identified between species. Concentrations of both growth factors were significantly greater in PRP samples obtained by use of the commercial kit versus those obtained by conical tube centrifugation. CONCLUSIONS AND CLINICAL RELEVANCE For blood samples from camelids, the commercial kit yielded a PRP product with a higher platelet and WBC concentration than achieved by conical tube centrifugation. Optimal PRP platelet and WBC concentrations for various applications need to be determined for llamas and alpacas.
Stacy A
SA
Semevolos
Cori D
CD
Youngblood
Stephanie K
SK
Grissom
M Elena
ME
Gorman
Maureen K
MK
Larson
D000818:Animals; D001781:Blood Component Removal / Q000379:methods / Q000662:veterinary*; D001792:Blood Platelets; D002161:Camelids, New World / Q000097:blood*; D002498:Centrifugation / Q000662:veterinary; D036341:Intercellular Signaling Peptides and Proteins / Q000097:blood; D053657:Platelet-Rich Plasma / Q000378:metabolism; D016212:Transforming Growth Factor beta / Q000097:blood
D036341:Intercellular Signaling Peptides and Proteins; D016212:Transforming Growth Factor beta