A study of DNA/BSA interaction and catalytic potential of oxidovanadium(v) complexes with ONO donor ligands.
The study of DNA/BSA interaction and the catalytic potential of four mononuclear oxidoalkoxido vanadium(v) [VVO(L1-4)OEt] (1-4) and one dinuclear oxidoalkoxido mixed-ligand vanadium(v) [{VO(L2)OEt}2(Q)]{Q = 4,4'-bipyridine}(5) complexes, with tridentate binegative aroylazine ligands are reported [where H2L1= anthranylhydrazone of 2-hydroxy-1-napthaldehyde, H2L2= salicylhydrazone of 2-hydroxy-1-napthaldehyde, H2L3= benzoylhydrazone of 2-hydroxy-1-acetonaphthone, H2L4= anthranylhydrazone of 2-hydroxy-1-acetonaphthone]. All the complexes are characterized by elemental analysis as well as various spectroscopic techniques. Single crystal X-ray diffraction crystallography of 2 reveals that the metal centre is in distorted square pyramidal geometry with O4N coordination spheres, whereas 5 exhibits a distorted octahedral geometry around the metal center. In addition, all the complexes (1-5) show moderate DNA binding propensity which is investigated using UV-vis absorption titration, circular dichroism, thermal denaturation and fluorescence spectral studies. The experimental results show that the complexes effectively interact with CT-DNA through both minor and major groove binding modes, with binding constants ranging from 104-105M-1. Among 1-5, complexes 3 and 4 show higher binding affinity towards CT-DNA than others and at the same time also exhibit negative ΔTmvalues of about ∼1.5 and 1.0 °C which resembles the properties shown by cisplatin. All complexes show moderate photo-induced cleavage of pUC19 supercoiled plasmid DNA with complex 3 showing the highest photo induced DNA cleavage activity of ∼48%. In coherence with the DNA interaction studies, 3 and 4 also exhibit good binding affinity towards BSA in the range of 1010-1011M-1, which is also supported by their ability to quench the tryptophan fluorescence emission spectra of BSA. All the complexes show remarkable photo-induced BSA cleavage activity (>90%) at a complex concentration of 50 μM. The catalytic potential of 1-5 is also tested for the oxidative bromination of styrene, salicylaldehyde and oxidation of methyl phenyl sulphide. All the reactions show a high percentage of conversion (>90%) with a high turnover frequency (TOF). Particularly, in the oxidative bromination of styrene the percentage of conversion and TOF vary from 96-98% and 8000-19 600 (h-1) respectively, which signifies the potential of these oxidovanadium(v) complexes to stimulate research for the synthesis of a better catalyst.
Department of Chemistry, National Institute of Technology, Rourkela 769008, Odisha, India. rupamdinda@nitrkl.ac.in and Department of Chemistry, Kuchinda College, Kuchinda, Sambalpur 768222, India.
Subhashree P
SP
Dash
School of Basic Sciences, Indian Institute of Technology Bhubaneswar, Bhubaneswar 751 013, Odisha, India.
Alok K
http://orcid.org/0000-0002-4452-993X
AK
Panda
Department of Chemistry, Indian Institute of Technology Roorkee, Roorkee 247667, India.
Sarita
S
Dhaka
Department of Chemistry, National Institute of Technology, Rourkela 769008, Odisha, India. rupamdinda@nitrkl.ac.in.
Sagarika
S
Pasayat
School of Basic Sciences, Indian Institute of Technology Bhubaneswar, Bhubaneswar 751 013, Odisha, India.
Ashis
A
Biswas
Department of Chemistry, Indian Institute of Technology Roorkee, Roorkee 247667, India.
Mannar R
MR
Maurya
Department of Chemistry, National Institute of Technology, Rourkela 769008, Odisha, India. rupamdinda@nitrkl.ac.in and Institute for Chemical Research, Kyoto University, Gokasho Uji, Kyoto 611-0011, Japan.
Paresh Kumar
PK
Majhi
Fribourg Center for Nanomaterials, Department of Chemistry, University of Fribourg, CH-1700 Fribourg, Switzerland.
Aurélien
A
Crochet
Department of Chemistry, National Institute of Technology, Rourkela 769008, Odisha, India. rupamdinda@nitrkl.ac.in.
Rupam
R
Dinda
D000447:Aldehydes / Q000737:chemistry; D002384:Catalysis; D002942:Circular Dichroism; D018360:Crystallography, X-Ray; D004247:DNA / Q000737:chemistry*; D053837:DNA Cleavage; D009682:Magnetic Resonance Spectroscopy; D009281:Naphthalenes / Q000737:chemistry; D009942:Organometallic Compounds / Q000138:chemical synthesis / Q000737:chemistry*; D055668:Photochemical Processes; D011485:Protein Binding; D011725:Pyridines / Q000737:chemistry; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D020058:Styrene / Q000737:chemistry; D014639:Vanadium / Q000737:chemistry*
D000447:Aldehydes; D009281:Naphthalenes; D009942:Organometallic Compounds; D011725:Pyridines; D014639:Vanadium; C013243:salicylaldehyde; D012710:Serum Albumin, Bovine; D020058:Styrene; D004247:DNA; C427203:calf thymus DNA; C521160:1-acetonaphthone; C034306:4,4'-bipyridyl