Viral interference of the bacterial RNA metabolism machinery.
In a recent publication, we reported a unique interaction between a protein encoded by the giant myovirus phiKZ and the Pseudomonas aeruginosa RNA degradosome. Crystallography, site-directed mutagenesis and interactomics approaches revealed this 'degradosome interacting protein' or Dip, to adopt an 'open-claw' dimeric structure that presents acidic patches on its outer surface which hijack 2 conserved RNA binding sites on the scaffold domain of the RNase E component of the RNA degradosome. This interaction prevents substrate RNAs from being bound and degraded by the RNA degradosome during the virus infection cycle. In this commentary, we provide a perspective into the biological role of Dip, its structural analysis and its mysterious evolutionary origin, and we suggest some therapeutic and biotechnological applications of this distinctive viral protein.
a Laboratory of Gene Technology , KU Leuven , Leuven , Belgium.|b Department of Biochemistry , University of Cambridge , Cambridge , UK.
Tom
T
Dendooven
a Laboratory of Gene Technology , KU Leuven , Leuven , Belgium.|c Division of Bacterial Diseases, Scientific Institute of Public Health (WIV-ISP) , Brussels , Belgium.
An
A
Van den Bossche
a Laboratory of Gene Technology , KU Leuven , Leuven , Belgium.
Hanne
H
Hendrix
a Laboratory of Gene Technology , KU Leuven , Leuven , Belgium.|c Division of Bacterial Diseases, Scientific Institute of Public Health (WIV-ISP) , Brussels , Belgium.
Pieter-Jan
PJ
Ceyssens
a Laboratory of Gene Technology , KU Leuven , Leuven , Belgium.
Marleen
M
Voet
b Department of Biochemistry , University of Cambridge , Cambridge , UK.
K J
KJ
Bandyra
d Biochemistry, Molecular and Structural Biology Section , KU Leuven , Leuven , Belgium.
Marc
M
De Maeyer
e Laboratory of Food Microbiology , KU Leuven , Leuven , Belgium.
Abram
A
Aertsen
f Biomedical Research Institute and Transnational University Limburg, Hasselt University , Diepenbeek , Belgium.
Jean-Paul
JP
Noben
b Department of Biochemistry , University of Cambridge , Cambridge , UK.
Steven W
SW
Hardwick
b Department of Biochemistry , University of Cambridge , Cambridge , UK.
Ben F
BF
Luisi
a Laboratory of Gene Technology , KU Leuven , Leuven , Belgium.
Rob
R
Lavigne
D001419:Bacteria / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism / Q000821:virology*; D001435:Bacteriophages / Q000502:physiology*; D004722:Endoribonucleases / Q000235:genetics / Q000378:metabolism; D054884:Host-Pathogen Interactions / Q000235:genetics*; D009097:Multienzyme Complexes / Q000235:genetics / Q000378:metabolism; D011117:Polyribonucleotide Nucleotidyltransferase / Q000235:genetics / Q000378:metabolism; D011485:Protein Binding; D011550:Pseudomonas aeruginosa / Q000502:physiology / Q000821:virology; D020365:RNA Helicases / Q000235:genetics / Q000378:metabolism; D020871:RNA Stability; D012329:RNA, Bacterial / Q000235:genetics* / Q000378:metabolism
D009097:Multienzyme Complexes; D012329:RNA, Bacterial; C111244:degradosome; D011117:Polyribonucleotide Nucleotidyltransferase; D004722:Endoribonucleases; D020365:RNA Helicases
Bacteriophage; Pseudomonas aeruginosa; RNA degradosome; RNase E; phage-host interaction