Murine models susceptibility to distinct Trypanosoma cruzi I genotypes infection.
Chagas disease is a complex zoonosis that affects around 8 million people worldwide. This pathology is caused by Trypanosoma cruzi, a kinetoplastid parasite that shows tremendous genetic diversity evinced in six distinct Discrete Typing Units (TcI-TcVI) including a recent genotype named as TcBat and associated with anthropogenic bats. TcI presents a broad geographical distribution and has been associated with chronic cardiomyopathy. Recent phylogenetic studies suggest the existence of two genotypes (Domestic (TcIDom) and sylvatic TcI) within TcI. The understanding of the course of the infection in different mouse models by these two genotypes is not yet known. Therefore, we infected 126 animals (ICR-CD1, National Institute of Health (NIH) and Balb/c) with two TcIDom strains and one sylvatic strain for a follow-up period of 60 days. We quantified the parasitaemia, immune response and histopathology observing that the maximum day of parasitaemia was achieved at day 21 post-infection. Domestic strains showed higher parasitaemia than the sylvatic strain in the three mouse models; however in the survival curves Balb/c mice were less susceptible to infection compared with NIH and ICR-CD1. Our results suggest that the genetic background plays a fundamental role in the natural history of the infection and the sympatric TcI genotypes have relevant implications in disease pathogenesis.
Grupo de Investigaciones Microbiológicas -UR (GIMUR), Programa de Biología, Facultad de Ciencias Naturales y Matemáticas,Universidad del Rosario,Bogotá,Colombia.
Cielo M
CM
León
Instituto Nacional de Salud,Bogotá,Colombia.
Marleny
M
Montilla
Instituto Nacional de Salud,Bogotá,Colombia.
Ricardo
R
Vanegas
Instituto Nacional de Salud,Bogotá,Colombia.
Maria
M
Castillo
Instituto Nacional de Salud,Bogotá,Colombia.
Edgar
E
Parra
Grupo de Investigaciones Microbiológicas -UR (GIMUR), Programa de Biología, Facultad de Ciencias Naturales y Matemáticas,Universidad del Rosario,Bogotá,Colombia.
Juan David
0000-0002-1344-9312
JD
Ramírez
D000818:Animals; D014355:Chagas Disease / Q000469:parasitology*; D004198:Disease Susceptibility; D005838:Genotype; D051379:Mice; D014349:Trypanosoma cruzi / Q000235:genetics*

               Trypanosoma cruzi
            ; Inbred line; mice