Distinct signaling programs control human hematopoietic stem cell survival and proliferation.
Several growth factors (GFs) that together promote quiescent human hematopoietic stem cell (HSC) expansion ex vivo have been identified; however, the molecular mechanisms by which these GFs regulate the survival, proliferation. and differentiation of human HSCs remain poorly understood. We now describe experiments in which we used mass cytometry to simultaneously measure multiple surface markers, transcription factors, active signaling intermediates, viability, and cell-cycle indicators in single CD34+cord blood cells before and up to 2 hours after their stimulation with stem cell factor, Fms-like tyrosine kinase 3 ligand, interleukin-3, interleukin-6, and granulocyte colony-stimulating factor (5 GFs) either alone or combined. Cells with a CD34+CD38-CD45RA-CD90+CD49f+(CD49f+) phenotype (∼10% HSCs with >6-month repopulating activity in immunodeficient mice) displayed rapid increases in activated STAT1/3/5, extracellular signal-regulated kinase 1/2, AKT, CREB, and S6 by 1 or more of these GFs, and β-catenin only when the 5 GFs were combined. Certain minority subsets within the CD49f+compartment were poorly GF-responsive and, among the more GF-responsive subsets of CD49f+cells, different signaling intermediates correlated with the levels of the myeloid- and lymphoid-associated transcription factors measured. Phenotypically similar, but CD90-CD49f-cells (MPPs) contained lower baseline levels of multiple signaling intermediates than the CD90+CD49f+cells, but showed similar response amplitudes to the same GFs. Importantly, we found activation or inhibition of AKT and β-catenin directly altered immediate CD49f+cell survival and proliferation. These findings identify rapid signaling events that 5 GFs elicit directly in the most primitive human hematopoietic cell types to promote their survival and proliferation.
Terry Fox Laboratory, British Columbia Cancer Agency, Vancouver, BC, Canada.
David J H F
http://orcid.org/0000-0002-2161-9959
DJ
Knapp
Terry Fox Laboratory, British Columbia Cancer Agency, Vancouver, BC, Canada.
Colin A
CA
Hammond
Baxter Laboratory for Stem Cell Biology, Department of Microbiology & Immunology, Stanford University, Palo Alto, CA.
Nima
N
Aghaeepour
Terry Fox Laboratory, British Columbia Cancer Agency, Vancouver, BC, Canada.
Paul H
PH
Miller
Terry Fox Laboratory, British Columbia Cancer Agency, Vancouver, BC, Canada.
Davide
D
Pellacani
Terry Fox Laboratory, British Columbia Cancer Agency, Vancouver, BC, Canada.
Philip A
PA
Beer
Baxter Laboratory for Stem Cell Biology, Department of Microbiology & Immunology, Stanford University, Palo Alto, CA.
Karen
K
Sachs
Terrence Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, Toronto, ON, Canada.
Wenlian
W
Qiao
Terrence Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, Toronto, ON, Canada.
WeiJia
W
Wang
Terry Fox Laboratory, British Columbia Cancer Agency, Vancouver, BC, Canada.
R Keith
RK
Humphries
Molecular Genetics of Stem Cells Laboratory, Institute of Research in Immunology and Cancer, University of Montreal, Montreal, QC, Canada.
Guy
G
Sauvageau
Terrence Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, Toronto, ON, Canada.
Peter W
PW
Zandstra
Department of Pathology, Stanford University, Palo Alto, CA; and.
Sean C
SC
Bendall
Baxter Laboratory for Stem Cell Biology, Department of Microbiology & Immunology, Stanford University, Palo Alto, CA.
Garry P
GP
Nolan
Centre for High-Throughput Biology, University of British Columbia, Vancouver, BC, Canada.
Carl
C
Hansen
Terry Fox Laboratory, British Columbia Cancer Agency, Vancouver, BC, Canada.
Connie J
CJ
Eaves
D000818:Animals; D049109:Cell Proliferation; D002470:Cell Survival; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D006801:Humans; D016130:Immunophenotyping; D036341:Intercellular Signaling Peptides and Proteins; D051379:Mice; D015398:Signal Transduction / Q000502:physiology*; D014157:Transcription Factors
D036341:Intercellular Signaling Peptides and Proteins; D014157:Transcription Factors