Production of the Functional Trisaccharide 1-Kestose from Cane Sugar Molasses Using Aspergillus japonicus β-Fructofuranosidase.
We report the production of the functional trisaccharide 1-kestose, O-β-D-fructofuranosyl-(2→1)-β-D-fructofuranosyl α-D-glucopyranoside, by β-fructofuranosidase from Aspergillus japonicus using sugar cane molasses as substrate. Sucrose in cane sugar molasses acted as a fructosyl donor and acceptor for the enzyme. The tetrasaccharide nystose, O-β-D-fructofuranosyl-(2→1)-β-D-fructofuranosyl-(2→1)-β-D-fructofuranosyl α-D-glucopyranoside, was produced from 1-kestose. Cane sugar molasses mixed with water provided a better substrate solution for β-fructofuranosidase compared to undiluted molasses due to the high concentration of product inhibitors such as glucose and fructose in molasses. The maximum concentration of 1-kestose obtained was 84.9 mg/ml and the maximum production efficiency was 32.3% after 24 h reaction at 40 °C. The maximum efficiency of combined fructo-oligosaccharide (1-kestose and nystose) production was 40.6%. 1-Kestose was therefore produced via a fructosyl-transfer reaction catalyzed by β-fructofuranosidase from A. japonicus.
Itochu Sugar Co. Ltd, 3 Tamatsuura, Hekinan-Shi, Aichi, 447-8506, Japan.|The Interdisciplinary Graduate School of Agriculture and Engineering, University of Miyazaki, 1-1 Gakuen Kibanadai Nishi, Miyazaki-Shi, Miyazaki, 889-2192, Japan.
Katsuki
K
Hirabayashi
Itochu Sugar Co. Ltd, 3 Tamatsuura, Hekinan-Shi, Aichi, 447-8506, Japan.
Nobuhiro
N
Kondo
Daiichi-Togyo Co. Ltd, 17371 Hichiya, Hyuga-Shi, Miyazaki, 883-0062, Japan.
Hiroshi
H
Toyota
The Interdisciplinary Graduate School of Agriculture and Engineering, University of Miyazaki, 1-1 Gakuen Kibanadai Nishi, Miyazaki-Shi, Miyazaki, 889-2192, Japan. shayashi@cc.miyazaki-u.ac.jp.
Sachio
S
Hayashi
D001230:Aspergillus / Q000201:enzymology*; D055162:Biocatalysis; D005656:Fungal Proteins / Q000737:chemistry* / Q000378:metabolism; D007700:Kinetics; D008965:Molasses / Q000032:analysis*; D009844:Oligosaccharides / Q000737:chemistry* / Q000378:metabolism; D031786:Saccharum / Q000378:metabolism / Q000382:microbiology; D014312:Trisaccharides / Q000737:chemistry* / Q000378:metabolism; D043324:beta-Fructofuranosidase / Q000737:chemistry* / Q000378:metabolism
D005656:Fungal Proteins; D009844:Oligosaccharides; D014312:Trisaccharides; C072433:1-kestose; C043600:fungitetraose; D043324:beta-Fructofuranosidase