Analysis of the Functionality of the Mitotic Checkpoints.
During cell division the main goal of the cell is to produce two daughter cells with the same genome as the mother, i.e., maintain its genetic stability. Since this issue is essential to preserve the cell ability to proliferate properly, all eukaryotic cells have developed several pathways, called mitotic checkpoints, that regulate mitotic entry, progression, and exit in response to different cellular signals. Given the evolutive conservation of mechanisms and proteins involved in the cell cycle control from yeast to humans, the budding yeast S. cerevisiae has been very helpful to gain insight in these complex regulations. Here, we describe how the checkpoint can be activated and which cellular phenotypes can be used as markers of checkpoint activation.
Dipartimento di Biotecnologie e Bioscienze, Università degli Studi di Milano-Bicocca, Piazza della Scienza 2, Milano, 20126, Italy. roberta.fraschini@unimib.it.
Roberta
R
Fraschini
D004271:DNA, Fungal / Q000032:analysis / Q000235:genetics; D004398:Dyneins / Q000032:analysis / Q000235:genetics / Q000378:metabolism; D005434:Flow Cytometry / Q000379:methods; D059566:M Phase Cell Cycle Checkpoints; D008853:Microscopy / Q000379:methods; D008938:Mitosis; D009154:Mutation; D012441:Saccharomyces cerevisiae / Q000166:cytology* / Q000235:genetics / Q000378:metabolism / Q000648:ultrastructure; D029701:Saccharomyces cerevisiae Proteins / Q000032:analysis / Q000235:genetics / Q000378:metabolism; D008941:Spindle Apparatus / Q000235:genetics / Q000378:metabolism / Q000648:ultrastructure
D004271:DNA, Fungal; D029701:Saccharomyces cerevisiae Proteins; C000596030:DYN1 protein, S cerevisiae; D004398:Dyneins
Cell cycle; Cellular morphology; DNA content; MEN; Mitotic spindle; Nuclear positioning