Understanding the immune system architecture and transcriptome responses to southern rice black-streaked dwarf virus in Sogatella furcifera.
Sogatella furcifera, the white-backed planthopper (WBPH), has become one of the most destructive pests in rice production owing to its plant sap-sucking behavior and efficient transmission of Southern rice black-streaked dwarf virus (SRBSDV) in a circulative, propagative and persistent manner. The dynamic and complex SRBSDV-WBPH-rice plant interaction is still poorly understood. In this study, based on a homology-based genome-wide analysis, 348 immune-related genes belonging to 28 families were identified in WBPH. A transcriptome analysis of non-viruliferous (NVF) and viruliferous groups with high viral titers (HVT) and median viral titers (MVT) revealed that feeding on SRBSDV-infected rice plants has a significant impact on gene expression, regardless of viral titers in insects. We identified 278 up-regulated and 406 down-regulated genes shared among the NVF, MVT, and HVT groups and detected significant down-regulation of primary metabolism-related genes and oxidoreductase. In viruliferous WBPH with viral titer-specific transcriptome changes, 1,906 and 1,467 genes exhibited strict monotonically increasing and decreasing expression, respectively. The RNAi pathway was the major antiviral response to increasing viral titers among diverse immune responses. These results clarify the responses of immune genes and the transcriptome of WBPH to SRBSDV and improve our knowledge of the functional relationship between pathogen, vector, and host.
School of Life Sciences, University of Science and Technology of China, Hefei, Anhui 230027, China.
Lin
L
Wang
School of Life Sciences, University of Science and Technology of China, Hefei, Anhui 230027, China.
Nan
N
Tang
School of Life Sciences, University of Science and Technology of China, Hefei, Anhui 230027, China.
Xinlei
X
Gao
School of Life Sciences, University of Science and Technology of China, Hefei, Anhui 230027, China.
Dongyang
D
Guo
School of Life Sciences, University of Science and Technology of China, Hefei, Anhui 230027, China.
Zhaoxia
Z
Chang
School of Life Sciences, University of Science and Technology of China, Hefei, Anhui 230027, China.
Yating
Y
Fu
School of Life Sciences, University of Science and Technology of China, Hefei, Anhui 230027, China.
Ibukun A
IA
Akinyemi
School of Life Sciences, University of Science and Technology of China, Hefei, Anhui 230027, China.|Hefei National Laboratory for Physical Sciences at the Microscale, Bio-X Interdisciplinary Sciences, 443 Huang-Shan Road, Hefei, Anhui 230027, China.
Qingfa
Q
Wu
D000818:Animals; D018506:Gene Expression Regulation, Plant; D063990:Gene Ontology; D006430:Hemiptera / Q000235:genetics* / Q000276:immunology / Q000821:virology*; D054884:Host-Pathogen Interactions; D007107:Immune System / Q000276:immunology*; D007109:Immunity / Q000235:genetics / Q000276:immunology; D007303:Insect Vectors / Q000235:genetics / Q000276:immunology / Q000821:virology; D012275:Oryza / Q000235:genetics / Q000469:parasitology / Q000821:virology; D010935:Plant Diseases / Q000235:genetics / Q000469:parasitology / Q000821:virology; D012087:Reoviridae / Q000502:physiology*; D015398:Signal Transduction / Q000235:genetics / Q000276:immunology; D059467:Transcriptome; D019562:Viral Load