Promotion of the transdermal delivery of protein drugs byN-trimethyl chitosan nanoparticles combined with polypropylene electret.
We recently reported that electret, which was prepared by a corona charging system with polypropylene film, could enhance the transdermal delivery of several drugs of low molecular weight. The aim of this study was to investigate whether electret could enhance the transdermal delivery of protein drugs byN-trimethyl chitosan nanoparticles (TMC NPs) prepared by an ionic gelation method. A series of experiments were performed, including in vitro skin permeation assays and anti-inflammatory effects, to evaluate the transdermal delivery of protein drugs by TMC NPs in the presence of electret. The results showed that in the presence of electret, the transdermal delivery of protein drugs in TMC NPs was significantly enhanced, as demonstrated by in vitro permeation studies and confocal laser scanning microscopy. Notably, superoxide dismutase-loaded TMC NPs combined with electret exhibited the best inhibitory effect on the edema of the mouse ear. TMC NPs combined with electret represent a novel platform for the transdermal delivery of protein drugs.
Department of Medical Affairs, East Hospital, Tongji University School of Medicine; Department of Pharmaceutical Science, School of Pharmacy, Second Military Medical University.
Ye
Y
Tu
Department of Pharmacy, East Hospital of Hepatobiliary Surgery.
Xinxia
X
Wang
Department of Pharmaceutical Science, School of Pharmacy, Second Military Medical University.
Ying
Y
Lu
Department of Pharmaceutical Science, School of Pharmacy, Second Military Medical University.
He
H
Zhang
Department of Pharmaceutical Science, School of Pharmacy, Second Military Medical University.
Yuan
Y
Yu
Department of Pharmaceutical Science, School of Pharmacy, Second Military Medical University.
Yan
Y
Chen
Department of Pharmaceutical Science, School of Pharmacy, Second Military Medical University.
Junjie
J
Liu
Department of Pharmaceutical Science, School of Pharmacy, Second Military Medical University.
Zhiguo
Z
Sun
Department of Inorganic Chemistry, School of Pharmacy, Second Military Medical University, Shanghai, People's Republic of China.
Lili
L
Cui
Department of Pharmaceutical Science, School of Pharmacy, Second Military Medical University.
Jing
J
Gao
Department of Pharmaceutical Science, School of Pharmacy, Second Military Medical University.
Yanqiang
Y
Zhong
D000279:Administration, Cutaneous; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000737:chemistry; D048271:Chitosan / Q000737:chemistry*; D004337:Drug Carriers / Q000494:pharmacology*; D016503:Drug Delivery Systems; D004487:Edema / Q000378:metabolism / Q000473:pathology; D005782:Gels; D007477:Ions; D007633:Keratins / Q000378:metabolism; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D018613:Microscopy, Confocal; D050997:Nanomedicine / Q000379:methods; D053758:Nanoparticles / Q000737:chemistry*; D010316:Particle Size; D011126:Polypropylenes / Q000737:chemistry*; D011506:Proteins; D051381:Rats; D017207:Rats, Sprague-Dawley; D012867:Skin / Q000187:drug effects*; D013482:Superoxide Dismutase / Q000737:chemistry / Q000378:metabolism
D000893:Anti-Inflammatory Agents; D004337:Drug Carriers; D005782:Gels; D007477:Ions; C118071:N-trimethyl chitosan chloride; D011126:Polypropylenes; D011506:Proteins; D007633:Keratins; D048271:Chitosan; D013482:Superoxide Dismutase
N-trimethyl chitosan nanoparticles; electret; protein drugs; transdermal delivery