Whole-brain ex-vivo quantitative MRI of the cuprizone mouse model.
Myelin is a critical component of the nervous system and a major contributor to contrast in Magnetic Resonance (MR) images. However, the precise contribution of myelination to multiple MR modalities is still under debate. The cuprizone mouse is a well-established model of demyelination that has been used in several MR studies, but these have often imaged only a single slice and analysed a small region of interest in the corpus callosum. We imaged and analyzed the whole brain of the cuprizone mouse ex-vivo using high-resolution quantitative MR methods (multi-component relaxometry, Diffusion Tensor Imaging (DTI) and morphometry) and found changes in multiple regions, including the corpus callosum, cerebellum, thalamus and hippocampus. The presence of inflammation, confirmed with histology, presents difficulties in isolating the sensitivity and specificity of these MR methods to demyelination using this model.
Department of Neuroimaging, IOPPN, King's College London , London , United Kingdom.
Tobias C
0000-0001-7640-5520
TC
Wood
Department of Neuroimaging, IOPPN, King's College London , London , United Kingdom.
Camilla
0000-0002-8561-4241
C
Simmons
FMRIB Centre, Nuffield Department of Clinical Neurosciences, University of Oxford, Oxford, Oxfordshire, United Kingdom; Synaptive Medical, Toronto, ON, Canada.
Samuel A
SA
Hurley
Cells and Behaviour Unit, Department of Basic and Clinical Neuroscience, IOPPN, King's College London , London , United Kingdom.
Anthony C
AC
Vernon
Department of Neuroimaging, IOPPN, King's College London , London , United Kingdom.
Joel
J
Torres
Department of Neuroimaging, IOPPN, King's College London, London, United Kingdom; NatBrainLab, Department of Basic and Clinical Neuroscience, IOPPN, King's College London, London, United Kingdom.
Flavio
F
Dell'Acqua
Department of Neuroimaging, IOPPN, King's College London , London , United Kingdom.
Steve C R
SC
Williams
Department of Neuroimaging, IOPPN, King's College London , London , United Kingdom.
Diana
D
Cash
Cuprizone; Inflammation; MRI; Mouse; Myelin; Quantitative imaging