Efficient utilization of Eucheuma denticulatum hydrolysates using an activated carbon adsorption process for ethanol production in a 5-L fermentor.
A total monosaccharide concentration of 37.8 g/L and 85.9% conversion from total fermentable monosaccharides of 44.0 g/L from 110 g dw/L Eucheuma denticulatum slurry were obtained by thermal acid hydrolysis and enzymatic saccharification. Subsequent adsorption treatment to remove 5-hydroxymethylfurfural (5-HMF) using 5% activated carbon and an adsorption time of 10 min were used to prevent a prolonged lag phase, reduced cell growth, and low ethanol production. The equilibrium adsorption capacity (qe) of HMF (58.183 mg/g) showed high affinity to activated carbon comparing to those of galactose (2.466 mg/g) and glucose (2.474 mg/g). The efficiency of cell growth and ethanol production with activated carbon treatment was higher than that without activated carbon treatment. Fermentation using S. stipitis KCTC7228 produced a cell concentration of 3.58 g dw/L with YX/Sof 0.107, and an ethanol concentration of 15.8 g/L with YP/Sof 0.48 in 96 h.
Department of Biotechnology, Pukyong National University, Busan, 48513, Korea.
Chae Hun
CH
Ra
Federation of Busan Science and Technology, Gijang-gun, Busan, 46048, Korea.
Min Ji
MJ
Kim
Department of Biotechnology, Pukyong National University, Busan, 48513, Korea.
Gwi-Taek
GT
Jeong
Department of Biotechnology, Pukyong National University, Busan, 48513, Korea. skkim@pknu.ac.kr.
Sung-Koo
SK
Kim
D000143:Acids / Q000737:chemistry; D000255:Adenosine Triphosphate / Q000737:chemistry; D000327:Adsorption; D018533:Biomass; D002244:Carbon / Q000737:chemistry*; D000431:Ethanol / Q000737:chemistry*; D005285:Fermentation; D005662:Furaldehyde / Q000031:analogs & derivatives* / Q000737:chemistry; D005947:Glucose / Q000737:chemistry; D006019:Glycolysis; D006868:Hydrolysis; D007218:Industrial Microbiology; D009005:Monosaccharides / Q000737:chemistry; D000461:Rhodophyta / Q000378:metabolism*; D012624:Seaweed; D013464:Sulfuric Acids / Q000737:chemistry
D000143:Acids; D009005:Monosaccharides; D013464:Sulfuric Acids; D000431:Ethanol; C008046:5-hydroxymethylfurfural; D002244:Carbon; D000255:Adenosine Triphosphate; D005662:Furaldehyde; D005947:Glucose; C033158:sulfuric acid
Activated carbon; Adsorption isotherm; Ethanol fermentation; Eucheuma denticulatum; Scheffersomyces stipitis KCTC 7228