Towards reconstruction of epithelialized cartilages from autologous adipose tissue-derived stem cells.
Deformities of the upper airways, including those of the nose and throat, are typically corrected by reconstructive surgery. The use of autologous somatic stem cells for repair of defects could improve quality and outcomes of such operations. The present study explored the ability of paediatric adipose-derived stem cells (pADSCs), a readily available source of autologous stem cells, to generate a cartilage construct with a functional epithelium. Paediatric ADSCs seeded on the biodegradable nanocomposite polymer, polyhedral oligomeric silsesquioxane poly(ϵ-caprolactone-urea) urethane (POSS-PCL), proliferated and differentiated towards mesenchymal lineages. The ADSCs infiltrated three-dimensional POSS-PCL nanoscaffold and chondroid matrix was observed throughout chondrogenically induced samples. In ovo chorioallantoic membrane-grafted ADSC-nanoscaffold composites were enwrapped by host vessels indicating good compatibility in an in vivo system. Furthermore, pADSCs could be induced to transdifferentiate towards barrier-forming epithelial-like cells. By combining differentiation protocols, it was possible to generate epithelial cell lined chondrogenic micromasses from the same pADSC line. This proof-of-concept study appears to be the first to demonstrate that individual pADSC lines can differentiate towards two different germ lines and be successfully co-cultured. This has important implications for bioengineering of paediatric airways and further confirms the plastic nature of ADSCs. Copyright © 2016 John Wiley & Sons, Ltd.
Stem Cell and Regenerative Medicine Section, UCL Great Ormond Street Institute of Child Health, University College London (UCL), London, UK.
Sophie E P
SEP
New
Stem Cell and Regenerative Medicine Section, UCL Great Ormond Street Institute of Child Health, University College London (UCL), London, UK.|UCL Ear Institute, Royal National Throat, Nose and Ear Hospital, UCL, London, UK.
Amel
A
Ibrahim
Centre for Endocrinology, William Harvey Research Institute, Barts and the London, Queen Mary University of London, London, UK.
Leonardo
L
Guasti
Stem Cell and Regenerative Medicine Section, UCL Great Ormond Street Institute of Child Health, University College London (UCL), London, UK.
Eleonora
E
Zucchelli
UCL Ear Institute, Royal National Throat, Nose and Ear Hospital, UCL, London, UK.
Martin
M
Birchall
Department of Plastic Surgery, Great Ormond Street Hospital NHS Trust, London, UK.
Neil W
NW
Bulstrode
Centre for Nanotechnology and Regenerative Medicine, Division of Surgery and Interventional Science, UCL, London, UK.
Alexander M
AM
Seifalian
Stem Cell and Regenerative Medicine Section, UCL Great Ormond Street Institute of Child Health, University College London (UCL), London, UK.
Patrizia
P
Ferretti
D000273:Adipose Tissue / Q000166:cytology / Q000378:metabolism*; D000293:Adolescent; D002356:Cartilage / Q000166:cytology / Q000378:metabolism*; D002648:Child; D002675:Child, Preschool; D005260:Female; D006801:Humans; D007223:Infant; D008297:Male; D017646:Organosilicon Compounds / Q000737:chemistry; D011091:Polyesters / Q000737:chemistry; D011140:Polyurethanes / Q000737:chemistry; D013234:Stem Cells / Q000166:cytology / Q000378:metabolism*; D054457:Tissue Scaffolds / Q000737:chemistry*
D017646:Organosilicon Compounds; D011091:Polyesters; D011140:Polyurethanes; C521417:polyhedraloligosilsesquioxane; C016240:polycaprolactone
adipose tissue derived stem cell (ADSC); cartilage; differentiation; epithelium; human; nanomaterial