Enterovirus-71 genotype C isolated in Peru between 2006 and 2009.
Enterovirus-71 (EV71) was first isolated in California, United States in 1969, belongs to the genus Enterovirus, family Picornaviridae. Although infection normally causes mild, often undiagnosed illness, it can cause central nervous system infections that could turn fatal. Based on VP1 gene analysis, EV71 has been classified into six separate genotypes. Although the molecular epidemiology of EV71 has been well described via studies originating from Asia and Europe, it is mostly unknown in South America. From our study, four EV71 isolates from Peru were characterized using phylogenetic methods to determine their relationship with known reference strains. These four Peruvian EV71 isolates from between 2006 and 2009 were analyzed by RT-PCR using primers capable of amplifying the entire VP1 gene. Reference strains representing all six known genotypes were used to determine any recognizable phylogenetic relationships. In fact, all of our isolates clustered together within the genotype C1 lineage- separate from Asian, European, North American, and Australian strains. We present evidence that EV71 genotype C1 exists in Peru, and this is the first such report documenting EV71 genotype C1 circulating in South America. Gathering additional isolates will help elucidate a more complete global epidemiological picture of EV71 infections.
U.S. Naval Medical Research Unit No. 6, Lima, Peru. Electronic address: jose.l.huaman2.fn@mail.mil.
Jose L
JL
Huaman
U.S. Naval Medical Research Unit No. 6, Lima, Peru. Electronic address: gladys.r.carrion.fn@mail.mil.
Gladys
G
Carrion
U.S. Naval Medical Research Unit No. 6, Lima, Peru. Electronic address: julia.s.ampuero.fn@mail.mil.
Julia S
JS
Ampuero
Centro de Salud Pachitea, Dirección Regional de Salud de Piura, Ministerio de Salud, Piura, Peru. Electronic address: vocanag01@yahoo.es.
Victor
V
Ocaña
Instituto de Medicina Tropical Daniel Alcides Carrión, UNMSM, Lima, Peru. Electronic address: laguna_alberto@hotmail.com.
V Alberto
VA
Laguna-Torres
U.S. Naval Medical Research Unit No. 6, Lima, Peru. Electronic address: robert.d.hontz.mil@mail.mil.
Robert D
RD
Hontz
D016000:Cluster Analysis; D029821:Enterovirus A, Human / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D004769:Enterovirus Infections / Q000453:epidemiology / Q000821:virology*; D005838:Genotype; D006801:Humans; D010568:Peru / Q000453:epidemiology; D010802:Phylogeny; D017422:Sequence Analysis, DNA; D015678:Viral Structural Proteins / Q000235:genetics
D015678:Viral Structural Proteins
Enterovirus-71; Genotype C1; ILI; Influenza like-illness; Latin America; Peru