The yeast Scheffersomyces amazonensis is an efficient xylitol producer.
This study assessed the efficiency of Scheffersomyces amazonensis UFMG-CM-Y493T, cultured in xylose-supplemented medium (YPX) and rice hull hydrolysate (RHH), to convert xylose to xylitol under moderate and severe oxygen limitation. The highest xylitol yields of 0.75 and 1.04 g g-1in YPX and RHH, respectively, were obtained under severe oxygen limitation. However, volumetric productivity in RHH was ninefold decrease than that in YPX medium. The xylose reductase (XR) and xylitol dehydrogenase (XDH) activities in the YPX cultures were strictly dependent on NADPH and NAD+respectively, and were approximately 10% higher under severe oxygen limitation than under moderate oxygen limitation. This higher xylitol production observed under severe oxygen limitation can be attributed to the higher XR activity and shortage of the NAD+needed by XDH. These results suggest that Sc. amazonensis UFMG-CM-Y493Tis one of the greatest xylitol producers described to date and reveal its potential use in the biotechnological production of xylitol.
Departamento de Microbiologia, Instituto de Ciências Biológicas (ICB), Universidade Federal de Minas Gerais, C.P. 486, Belo Horizonte, MG, 31270-901, Brazil.|Laboratório Nacional de Energia e Geologia, I.P., Unidade de Bioenergia, Estrada do Paço do Lumiar 22, 1649-038, Lisbon, Portugal.
Raquel M
RM
Cadete
Departamento de Microbiologia, Instituto de Ciências Biológicas (ICB), Universidade Federal de Minas Gerais, C.P. 486, Belo Horizonte, MG, 31270-901, Brazil.
Monaliza A
MA
Melo-Cheab
Departamento de Alimentos, Faculdade de Farmácia, Universidade Federal de Minas Gerais, Belo Horizonte, MG, 31270-901, Brazil.
Adriana L
AL
Viana
Departamento de Alimentos, Faculdade de Farmácia, Universidade Federal de Minas Gerais, Belo Horizonte, MG, 31270-901, Brazil.
Evelyn S
ES
Oliveira
Laboratório Nacional de Energia e Geologia, I.P., Unidade de Bioenergia, Estrada do Paço do Lumiar 22, 1649-038, Lisbon, Portugal.|Section for Sustainable Biotechnology, Aalborg University Copenhagen, A.C. Meyers Vænge 15, 2450, Copenhagen SV, Denmark.
César
C
Fonseca
Departamento de Microbiologia, Instituto de Ciências Biológicas (ICB), Universidade Federal de Minas Gerais, C.P. 486, Belo Horizonte, MG, 31270-901, Brazil. carlrosa@icb.ufmg.br.
Carlos A
CA
Rosa
D000449:Aldehyde Reductase / Q000378:metabolism; D003470:Culture Media / Q000737:chemistry; D050542:D-Xylulose Reductase / Q000378:metabolism; D055165:Debaryomyces / Q000145:classification / Q000201:enzymology / Q000254:growth & development*; D005285:Fermentation; D005656:Fungal Proteins / Q000378:metabolism; D007218:Industrial Microbiology; D009243:NAD / Q000378:metabolism; D009249:NADP / Q000378:metabolism; D014993:Xylitol / Q000096:biosynthesis* / Q000378:metabolism; D014994:Xylose / Q000378:metabolism
D003470:Culture Media; D005656:Fungal Proteins; D009243:NAD; D009249:NADP; D014994:Xylose; D000449:Aldehyde Reductase; D050542:D-Xylulose Reductase; D014993:Xylitol
Bioconversion; D-Xylose; Hemicellulose hydrolysates; Scheffersomyces amazonensis; Xylitol