Design, synthesis and transfection efficiency of a novel redox-sensitive polycationic amphiphile.
A novel redox-sensitive polycationic amphiphile (2S3) with disulphide linkers for nucleic acid delivery was developed. Cationic liposomes formed by 2S3 and the helper lipid DOPE demonstrated effective DNA delivery into HEK293 cells with a maximal transfection activity that is superior than both nonredox-sensitive cationic liposomes and Lipofectamine® 2000 at an N/P ratio of 6/1. Redox-sensitivity was tested by experiments with extracellular glutathione which shown the ability of disulphide linker degradation. Our results suggest that polycationic amphiphile 2S3 is a promising candidate for nucleic acid delivery.
Institute of Fine Chemical Technologies, Moscow Technological University, Vernadsky ave. 86, Moscow 119571, Russia.
Pavel A
PA
Puchkov
Institute of Fine Chemical Technologies, Moscow Technological University, Vernadsky ave. 86, Moscow 119571, Russia.
Elena V
EV
Shmendel
Institute of Fine Chemical Technologies, Moscow Technological University, Vernadsky ave. 86, Moscow 119571, Russia.
Anastasya S
AS
Luneva
Institute of Fine Chemical Technologies, Moscow Technological University, Vernadsky ave. 86, Moscow 119571, Russia.
Nina G
NG
Morozova
Institute of Chemical Biology and Fundamental Medicine SB RAS, Lavrentiev ave. 8, Novosibirsk 630090, Russia.
Marina A
MA
Zenkova
Institute of Fine Chemical Technologies, Moscow Technological University, Vernadsky ave. 86, Moscow 119571, Russia. Electronic address: mamaslov@mail.ru.
Mikhail A
MA
Maslov
D004247:DNA / Q000235:genetics* / Q000378:metabolism; D015195:Drug Design; D018014:Gene Transfer Techniques; D057809:HEK293 Cells; D006801:Humans; D015394:Molecular Structure; D010084:Oxidation-Reduction; D011073:Polyamines / Q000138:chemical synthesis / Q000737:chemistry*; D000071228:Polyelectrolytes; D013501:Surface-Active Agents / Q000138:chemical synthesis / Q000737:chemistry*; D014162:Transfection
D004247:DNA; D011073:Polyamines; D000071228:Polyelectrolytes; D013501:Surface-Active Agents
Cationic liposomes; Disulphide linker; Gemini amphiphiles; Gene therapy; Redox-sensitive