Determination of AZD6118 in dog plasma samples utilizing microextraction by packed sorbent and liquid chromatography-electrospray ionization tandem mass spectrometry.
In this work, for the first time, a method has been developed for the determination of AZD6118, a candidate drug, in dog plasma samples. The method is based on microextraction by packed sorbent (MEPS) of the drug prior to liquid chromatography-electrospray ionization tandem mass spectrometry assay. Various important factors affecting MEPS performance were optimized, and under the optimized condition, a linear calibration curve in the concentration range of 20-25,000nmolL-1with a coefficient of determination over 0.99 was obtained. The back-calculated values of the calibration points showed good agreement with the theoretical concentrations (coefficients of variation percent between 0.3-3.8). The lower limit of quantification and limit of detection were 20.0 and 2.9nmolL-1, respectively. The repeatability and accuracy of the method was evaluated by determination of quality control samples at three concentration levels (low, medium and high) using the developed method, and the results (coefficients of variation values were between 1.9% and 3.2%, relative recoveries ranged between 93.5-102.1%) confirm that a powerful method has been developed for the extraction and determination of the investigated drug in dog plasma.
Department of Environmental Sci. & Analytical Chemistry, Stockholm University, SE10691 Stockholm, Sweden.
Aziza
A
El Beqqali
Department of Environmental Sci. & Analytical Chemistry, Stockholm University, SE10691 Stockholm, Sweden.
Mazaher
M
Ahmadi
Department of Environmental Sci. & Analytical Chemistry, Stockholm University, SE10691 Stockholm, Sweden. Electronic address: Mohamed.abdel-rehim@aces.su.se.
Mohamed
M
Abdel-Rehim
D000818:Animals; D002853:Chromatography, Liquid / Q000379:methods*; D004285:Dogs; D015507:Drugs, Investigational / Q000032:analysis* / Q000737:chemistry / Q000493:pharmacokinetics / Q000633:toxicity; D005260:Female; D057230:Limit of Detection; D016014:Linear Models; D008297:Male; D015203:Reproducibility of Results; D052617:Solid Phase Microextraction / Q000379:methods*; D053719:Tandem Mass Spectrometry / Q000379:methods*
D015507:Drugs, Investigational
AZD6118; Dog plasma samples; LC-ESI–MS/MS; Microextraction by packed sorbent; Validation