Novel amperometric glucose biosensor based on MXene nanocomposite.
A biosensor platform based on Au/MXene nanocomposite for sensitive enzymatic glucose detection is reported. The biosensor leverages the unique electrocatalytic properties and synergistic effects between Au nanoparticles and MXene sheets. An amperometric glucose biosensor is fabricated by the immobilization of glucose oxidase (GOx) enzyme on Nafion solubilized Au/ MXene nanocomposite over glassy carbon electrode (GCE). The biomediated Au nanoparticles play a significant role in facilitating the electron exchange between the electroactive center of GOx and the electrode. The GOx/Au/MXene/Nafion/GCE biosensor electrode displayed a linear amperometric response in the glucose concentration range from 0.1 to 18 mM with a relatively high sensitivity of 4.2 μAmM-1cm-2and a detection limit of 5.9 μM (S/N = 3). Furthermore, the biosensor exhibited excellent stability, reproducibility and repeatability. Therefore, the Au/MXene nanocomposite reported in this work is a potential candidate as an electrochemical transducer in electrochemical biosensors.
Materials Science and Engineering, King Abdullah University of Science and Technology, (KAUST), Thuwal 23955-6900, Saudi Arabia.|Chemical Sciences and Technology Division, CSIR-National Institute of Interdisciplinary Science and Technology (CSIR-NIIST), Thiruvananthapuram, 695019, India.
R B
RB
Rakhi
Materials Science and Engineering, King Abdullah University of Science and Technology, (KAUST), Thuwal 23955-6900, Saudi Arabia.
Pranati
P
Nayak
Materials Science and Engineering, King Abdullah University of Science and Technology, (KAUST), Thuwal 23955-6900, Saudi Arabia.
Chuan
C
Xia
Materials Science and Engineering, King Abdullah University of Science and Technology, (KAUST), Thuwal 23955-6900, Saudi Arabia.
Husam N
HN
Alshareef
D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D002244:Carbon / Q000737:chemistry; D055664:Electrochemical Techniques; D004566:Electrodes; D004800:Enzymes, Immobilized / Q000737:chemistry / Q000378:metabolism; D005465:Fluorocarbon Polymers / Q000737:chemistry; D005947:Glucose / Q000032:analysis*; D005949:Glucose Oxidase / Q000737:chemistry / Q000378:metabolism; D006046:Gold / Q000737:chemistry; D057230:Limit of Detection; D053761:Nanocomposites / Q000737:chemistry* / Q000648:ultrastructure
D004800:Enzymes, Immobilized; D005465:Fluorocarbon Polymers; C040402:perfluorosulfonic acid; D002244:Carbon; D006046:Gold; D005949:Glucose Oxidase; D005947:Glucose