Effect of Topical Administration of Fractions and Isolated Molecules from Plant Extracts on Skin Wound Healing: A Systematic Review of Murine Experimental Models.
Background and Purpose.Skin wound healing is a dynamic process driven by molecular events responsible for the morphofunctional repair of the injured tissue. In a systematic review, we analyzed the relevance of plant fractions and isolates on skin wound healing. By revising preclinical investigations with murine models, we investigated if the current evidence could support clinical trials.Methods.Studies were selected in the MEDLINE/PubMed and Scopus databases according to the PRISMA statement. All 32 identified studies were submitted to data extraction and the methodological bias was investigated according to ARRIVE strategy.Results.The studies demonstrated that plant fractions and isolates are able to modulate the inflammatory process during skin wound healing, being also effective in attenuating the oxidative tissue damage in the scar tissue and stimulating cell proliferation, neoangiogenesis, collagen synthesis, granulation tissue expansion, reepithelialization, and the wound closure rate. However, we identified serious methodological flaws in all studies, such as the high level of reporting bias and absence of standardized experimental designs, analytical methods, and outcome measures.Conclusion.Considering these limitations, the current evidence generated from flawed methodological animal studies makes it difficult to determine the relevance of herbal medicines to treat skin wounds and derails conducting clinical studies.
Department of General Biology, Federal University of Viçosa, 35570-000 Viçosa, MG, Brazil.
Mariáurea Matias
0000-0003-1313-7877
MM
Sarandy
Department of Animal Biology, Federal University of Viçosa, 35570-000 Viçosa, MG, Brazil.
Fernanda Barbosa
FB
Lopes
Department of General Biology, Federal University of Viçosa, 35570-000 Viçosa, MG, Brazil.
Sérgio Luis Pinto
0000-0003-3868-9475
SL
da Matta
Instituto Celulare, 25730-735 Petropolis, RJ, Brazil.
Marcus Vinicius Mello
MV
Pinto
Department of Animal Biology, Federal University of Viçosa, 35570-000 Viçosa, MG, Brazil.
Sirlene Souza Rodrigues
SS
Sartori
Institute of Biomedical Sciences, Department of Structural Biology, Federal University of Alfenas, 37130-000 Alfenas, MG, Brazil.
Rômulo Dias
0000-0002-3186-5328
RD
Novaes
Department of Animal Biology, Federal University of Viçosa, 35570-000 Viçosa, MG, Brazil.
Reggiani Vilela
0000-0002-5831-3590
RV
Golçalves
D000818:Animals; D051379:Mice; D010936:Plant Extracts / Q000627:therapeutic use*; D012871:Skin Diseases / Q000188:drug therapy*; D014945:Wound Healing / Q000187:drug effects*
D010936:Plant Extracts