MicroRNA-107 is downregulated and having tumor suppressive effect in breast cancer by negatively regulating brain-derived neurotrophic factor.
BACKGROUND
In the present study, we investigated the possible tumor suppressive effect of microRNA-107 (miR-107) in human breast cancer.


METHODS
Gene expression of miR-107 in breast cancer cell lines and clinical breast tissues was evaluated by a quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR). MiR-107 was stably overexpressed in MCF-7 and MDA-MB-231 cells via lentiviral transduction. Its role in regulating in vitro cancer proliferation, cell cycle, invasion and in vivo tumor growth was evaluated by MTT, flow cytometry, transwell and in vivo tumorigenicity assays, respectively. Association of miR-107 and its downstream target, brain-derived neurotrophic factor (BDNF), was evaluated by luciferase assay and qRT-PCR. BDNF was further upregulated in miR-107-overexpressed MCF-7 and MDA-MB-231 cells to evaluate its role in regulating breast cancer with respect to in vitro proliferation, cell cycle and invasion.


RESULTS
MiR-107 was markedly downregulated in both breast cancer cell lines and breast tumors. MiR-107 overexpression was markedly suppressed with respect to in vitro breast cancer proliferation, cell cycle progression and invasion, as well as with respect to in vivo development of breast cancer xenograft. BDNF was found to be the downstream target of miR-107 in breast cancer. BDNF upregulation functionally facilitated in vitro proliferation, cell cycle progression and invasion in miR-107-overexpressed breast cancer cells.


CONCLUSIONS
MiR-107 is downregulated and has a tumor suppressive effect in breast cancer, likely through regulation via its inverted downstream target of BDNF.
Department of Breast & Thyroid & Vascular Surgery, Research Institute of Surgery, Daping Hospital, Third Military Medical University, Chongqing, China.
Bo
B
Gao
Department of Breast & Thyroid & Vascular Surgery, Research Institute of Surgery, Daping Hospital, Third Military Medical University, Chongqing, China.
Shuai
S
Hao
Department of Breast & Thyroid & Vascular Surgery, Research Institute of Surgery, Daping Hospital, Third Military Medical University, Chongqing, China.
Wuguo
W
Tian
Department of Breast & Thyroid & Vascular Surgery, Research Institute of Surgery, Daping Hospital, Third Military Medical University, Chongqing, China.
Yan
Y
Jiang
Department of Breast & Thyroid & Vascular Surgery, Research Institute of Surgery, Daping Hospital, Third Military Medical University, Chongqing, China.
Shu
S
Zhang
Department of Breast & Thyroid & Vascular Surgery, Research Institute of Surgery, Daping Hospital, Third Military Medical University, Chongqing, China.
Lingji
L
Guo
Department of Breast & Thyroid & Vascular Surgery, Research Institute of Surgery, Daping Hospital, Third Military Medical University, Chongqing, China.
Jianjie
J
Zhao
Department of Breast & Thyroid & Vascular Surgery, Research Institute of Surgery, Daping Hospital, Third Military Medical University, Chongqing, China.
Gang
G
Zhang
Department of Breast & Thyroid & Vascular Surgery, Research Institute of Surgery, Daping Hospital, Third Military Medical University, Chongqing, China.
Jie
J
Yan
Department of Breast & Thyroid & Vascular Surgery, Research Institute of Surgery, Daping Hospital, Third Military Medical University, Chongqing, China.
Donglin
D
Luo
D020413:3' Untranslated Regions / Q000235:genetics; D000818:Animals; D001483:Base Sequence; D019208:Brain-Derived Neurotrophic Factor / Q000235:genetics* / Q000378:metabolism; D001943:Breast Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D002460:Cell Line; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000235:genetics; D015536:Down-Regulation; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D016147:Genes, Tumor Suppressor; D006801:Humans; D061986:MCF-7 Cells; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D035683:MicroRNAs / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid; D014183:Transplantation, Heterologous
D020413:3' Untranslated Regions; D019208:Brain-Derived Neurotrophic Factor; C549045:MIRN107 microRNA, human; D035683:MicroRNAs
BDNF; breast cancer; in vitro cancer assay; in vivo tumorigenicity; miR-107; qRT-PCR