Detection of the pH-dependent Activity of Escherichia coli Chaperone HdeB In Vitro and In Vivo.
Bacteria are frequently exposed to environmental changes, such as alterations in pH, temperature, redox status, light exposure or mechanical force. Many of these conditions cause protein unfolding in the cell and have detrimental impact on the survival of the organism. A group of unrelated, stress-specific molecular chaperones have been shown to play essential roles in the survival of these stress conditions. While fully folded and chaperone-inactive before stress, these proteins rapidly unfold and become chaperone-active under specific stress conditions. Once activated, these conditionally disordered chaperones bind to a large number of different aggregation-prone proteins, prevent their aggregation and either directly or indirectly facilitate protein refolding upon return to non-stress conditions. The primary approach for gaining a more detailed understanding about the mechanism of their activation and client recognition involves the purification and subsequent characterization of these proteins using in vitro chaperone assays. Follow-up in vivo stress assays are absolutely essential to independently confirm the obtained in vitro results. This protocol describes in vitro and in vivo methods to characterize the chaperone activity of E. coli HdeB, an acid-activated chaperone. Light scattering measurements were used as a convenient read-out for HdeB's capacity to prevent acid-induced aggregation of an established model client protein, MDH, in vitro. Analytical ultracentrifugation experiments were applied to reveal complex formation between HdeB and its client protein LDH, to shed light into the fate of client proteins upon their return to non-stress conditions. Enzymatic activity assays of the client proteins were conducted to monitor the effects of HdeB on pH-induced client inactivation and reactivation. Finally, survival studies were used to monitor the influence of HdeB's chaperone function in vivo.
Department of Molecular, Cellular, and Developmental Biology, University of Michigan; judahl@umich.edu.
Jan-Ulrik
JU
Dahl
Department of Molecular, Cellular, and Developmental Biology, University of Michigan; Howard Hughes Medical Institute, University of Michigan.
Philipp
P
Koldewey
Department of Molecular, Cellular, and Developmental Biology, University of Michigan; Howard Hughes Medical Institute, University of Michigan; jbardwel@umich.edu.
James C A
JC
Bardwell
Department of Molecular, Cellular, and Developmental Biology, University of Michigan.
Ursula
U
Jakob
D004926:Escherichia coli; D029968:Escherichia coli Proteins; D006863:Hydrogen-Ion Concentration; D018832:Molecular Chaperones; D011485:Protein Binding
D029968:Escherichia coli Proteins; D018832:Molecular Chaperones