Enhanced degradation of isoproturon in an agricultural soil by a Sphingomonas sp. strain and a microbial consortium.
Isoproturon (IPU) degradation in an agricultural soil inoculated with an isolated IPU-degrader strain (Sphingomonas sp. strain AK1, IS) or a microbial consortium (MC) harboring this strain, with or without carrier material, were investigated in soil microcosm experiments during 46 days. Effect of the carrier material and inoculation size on IPU-degradation efficacy of the inoculants were studied. Mineralization, extractable residues and non-extractable residues of14C-labeled IPU were analyzed. The low IPU mineralization in untreated soil (7.0%) was enhanced to different extents by inoculation of IS (17.4%-46.0%) or MC (58.9%-67.5%). Concentrations of IPU residues in soils amended with MC (0.002-0.095 μg g dry soil-1) were significantly lower than in soils amended with IS (0.02-0.67 μg g dry soil-1) and approximately 10 times lower than in the uninoculated soil (0.06-0.80 μg g dry soil-1). Less extractable residues and non-extractable residues were detected in soil with higher IPU mineralization. Inoculation size (as indicated by the volume of liquid cultures or by the number of carrier particles) determined the IPU-removal efficacy of IS in soil, but this effect was less pronounced for MC. The low sorption of IPU to soil and the decreasing IPU-mineralizing rates suggested incapability of IS to establish the IPU-mineralizing function in the soil. The thorough removal of IPU and persistent IPU-mineralizing activity of soil inoculated with MC indicated a high persistence of IPU-metabolic trait. Our results showed that microbial consortia might be more efficient than single degrader strains to enhance clean-up of organic chemicals in soil.
Research Unit Microbe-Plant Interactions, Helmholtz Zentrum München, German Research Center for Environmental Health (GmbH), 85764 Neuherberg, Germany. Electronic address: renyili2009@gmail.com.
Renyi
R
Li
Research Unit Microbe-Plant Interactions, Helmholtz Zentrum München, German Research Center for Environmental Health (GmbH), 85764 Neuherberg, Germany.
Ulrike
U
Dörfler
Lehrstuhl für Grünlandlehre, Technische Universität München, 85764 Neuherberg, Germany.
Jean Charles
JC
Munch
Research Unit Microbe-Plant Interactions, Helmholtz Zentrum München, German Research Center for Environmental Health (GmbH), 85764 Neuherberg, Germany. Electronic address: schroll@helmholtz-muenchen.de.
Reiner
R
Schroll
D000383:Agriculture; D001673:Biodegradation, Environmental; D004787:Environmental Pollution; D006540:Herbicides / Q000737:chemistry*; D059013:Microbial Consortia; D008903:Minerals / Q000378:metabolism; D010671:Phenylurea Compounds / Q000737:chemistry*; D012987:Soil / Q000737:chemistry*; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000032:analysis / Q000737:chemistry*; D020578:Sphingomonas / Q000378:metabolism*
D006540:Herbicides; D008903:Minerals; D010671:Phenylurea Compounds; D012987:Soil; D012989:Soil Pollutants; C028904:isoproturon
(14)C-label; Metabolites; Microbial consortium; Mineralization; Non-extractable residues; Pesticide