A novel method for sensitive microRNA detection: Electropolymerization based doping.
In the proposed study, for the first time, sensitive electrochemical detection of a breast cancer biomarker microRNA (miRNA), mir-21 was achieved via electropolymerized polypyrrole (PPy) modified pencil graphite electrodes (PPy/PGE). The detection of hybridization of electrochemically doped probe miRNA, antimir-21, with its complementary target, mir-21 was monitored by either electrochemical impedance spectroscopy (EIS) via comparison of charge transfer resistance (Rct) values before and after hybridization or by electrochemical reduction signal of an hybridization indicator, Meldola's blue (MDB). The study covers all the optimization steps for hybridization procedure and electropolymerization of pyrrole as well as detection from real samples of breast cancer cell line, MCF-7. The designed sensor shows a high selectivity and a low detection limit of 0.17nM thanks to electrical conductivity and porous structure of PPy.
Gediz University, Department of Biomedical Engineering, 35665 Seyrek, Turkey.
Merve
M
Kaplan
Swiss Federal Institute of Technology Lausanne, EPFL, Integrated Systems Laboratory, 1015 Lausanne, Switzerland. Electronic address: tugba.kilic@epfl.ch.
Tugba
T
Kilic
Center for Drug Research & Development and Pharmacokinetic Applications (ARGEFAR), Ege University, 35100 Izmir, Turkey.
Gunnur
G
Guler
Université Hassan II de Casablanca, Faculté des Sciences et Techniques, Laboratoire Génie des Procédés et Environnement, B.P. 146, 20800 Mohammedia, Morocco.
Jihane
J
Mandli
Université Hassan II de Casablanca, Faculté des Sciences et Techniques, Laboratoire Génie des Procédés et Environnement, B.P. 146, 20800 Mohammedia, Morocco.
Aziz
A
Amine
Gediz University, Department of Biomedical Engineering, 35665 Seyrek, Turkey. Electronic address: ozsozsg@gmail.com.
Mehmet
M
Ozsoz
D015374:Biosensing Techniques / Q000379:methods; D001943:Breast Neoplasms / Q000235:genetics; D058266:Dielectric Spectroscopy / Q000379:methods; D055664:Electrochemical Techniques / Q000379:methods*; D004566:Electrodes; D005260:Female; D006108:Graphite / Q000737:chemistry*; D006801:Humans; D057230:Limit of Detection; D061986:MCF-7 Cells; D035683:MicroRNAs / Q000032:analysis* / Q000235:genetics; D009693:Nucleic Acid Hybridization / Q000379:methods; D058105:Polymerization; D011108:Polymers / Q000737:chemistry*; D011758:Pyrroles / Q000737:chemistry*; D015347:RNA Probes / Q000737:chemistry / Q000235:genetics
C524940:MIRN21 microRNA, human; D035683:MicroRNAs; D011108:Polymers; D011758:Pyrroles; D015347:RNA Probes; C067635:polypyrrole; D006108:Graphite
Differential pulse voltammetry; Electrochemical impedance spectroscopy; Electropolymerization; Meldola's blue; Mir-21; Pyrrole; microRNA