Antitumor efficacy of combined gene and radiotherapy in animals.
Antitumor efficacy of the combined suicide gene therapy and radiotherapy was studied on the model of CT26 murine colon adenocarcinoma. CMV-FCU1-IRES-mGM-CSF-pGL3 construct with PEG-PEI-TAT (FCU1-mGM/5-FC) block copolymer as a vector was used for intratumoral administration. Tumors were irradiated with a single 5 Gy dose. The efficacy was evaluated according to the grade of tumor growth inhibition (T/C) and lifespan of the animals. Pronounced antitumor activity of the combined use of FCU1-mGM/5-FC system with radiotherapy on the background of prolonged lifespan and the synergism of the applied methods was revealed.
National Medical Research Radiological Center (NMRRC), Ministry of Healthcare of the Russian Federation, Obninsk, Kaluga oblast, Russia. olgabezborodova@yandex.ru.
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OA
Bezborodova
National Medical Research Radiological Center (NMRRC), Ministry of Healthcare of the Russian Federation, Obninsk, Kaluga oblast, Russia.
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ER
Nemtsova
National Medical Research Radiological Center (NMRRC), Ministry of Healthcare of the Russian Federation, Obninsk, Kaluga oblast, Russia.
A R
AR
Gevorkov
National Medical Research Radiological Center (NMRRC), Ministry of Healthcare of the Russian Federation, Obninsk, Kaluga oblast, Russia.
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Boyko
National Medical Research Radiological Center (NMRRC), Ministry of Healthcare of the Russian Federation, Obninsk, Kaluga oblast, Russia.
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JB
Venediktova
Institute of Molecular Genetics, Russian Academy of Sciences, pl. Akademika Kurchatova 46, Moscow, 123182, Russia.|Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, ul. Miklukho-Maklaya 16/10, Moscow, 117997, Russia.
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Alekseenko
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, ul. Miklukho-Maklaya 16/10, Moscow, 117997, Russia.
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Kostina
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, ul. Miklukho-Maklaya 16/10, Moscow, 117997, Russia.
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Monastyrskaya
Institute of Molecular Genetics, Russian Academy of Sciences, pl. Akademika Kurchatova 46, Moscow, 123182, Russia.|Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, ul. Miklukho-Maklaya 16/10, Moscow, 117997, Russia.
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Sverdlov
National Medical Research Radiological Center (NMRRC), Ministry of Healthcare of the Russian Federation, Obninsk, Kaluga oblast, Russia.
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EV
Khmelevskiy
National Medical Research Radiological Center (NMRRC), Ministry of Healthcare of the Russian Federation, Obninsk, Kaluga oblast, Russia.
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Yakubovskaya
D000230:Adenocarcinoma / Q000473:pathology / Q000628:therapy*; D000818:Animals; D000964:Antimetabolites, Antineoplastic / Q000008:administration & dosage; D045744:Cell Line, Tumor; D003110:Colonic Neoplasms / Q000473:pathology / Q000628:therapy*; D003131:Combined Modality Therapy / Q000379:methods; D003587:Cytomegalovirus / Q000235:genetics; D005437:Flucytosine / Q000008:administration & dosage; D005472:Fluorouracil / Q000008:administration & dosage; D041722:Genes, Transgenic, Suicide; D015316:Genetic Therapy / Q000379:methods*; D005822:Genetic Vectors; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000235:genetics; D008807:Mice, Inbred BALB C; D060787:Neoplasm Grading; D009368:Neoplasm Transplantation; D016896:Treatment Outcome; D047368:Tumor Burden
D000964:Antimetabolites, Antineoplastic; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D005437:Flucytosine; D005472:Fluorouracil