Characterization and antimicrobial susceptibility of one antibiotic-sensitive and one multidrug-resistantCorynebacterium kroppenstedtiistrain isolated from patients with granulomatous mastitis.
Human infections associated withCorynebacterium kroppenstedtiiare rarely reported, and this organism is usually described as antibiotic sensitive. Almost all published cases ofC. kroppenstedtiiinfections have been associated with breast pathology in women and have been described in New Zealand, France, Canada, India and Japan. Here we describe the microbiologic characteristics of two strains isolated from two women diagnosed of granulomatous mastitis in Spain. OneC. kroppenstedtiiisolate was antibiotic sensitive while the other was multidrug resistant. Biochemical identification was possible using a wide battery of methods including API Coryne V2.0, API Strep, API NH, API NE, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry and 16S rRNA gene amplification and sequencing. Antimicrobial susceptibility to 28 antibiotics as determined by Etest showed one isolate being sensitive to benzylpenicillin, ciprofloxacin, moxifloxacin, gentamicin, vancomycin, clindamycin, tetracycline, linezolid and rifampin. The second isolate showed resistance to ciprofloxacin, moxifloxacin, clindamycin, tetracycline and rifampin. The multidrug-resistant isolate contained theerm(X),tet(W),cmx, aphA1-IAB, strABandsul1resistance genes known from the R plasmid pJA144188 ofCorynebacterium resistens.These genes were absent in the genome of the antibiotic-sensitive isolate. This report confirms the tropism of this microorganism for women's breasts and presents the first description of a multidrug-resistantC. kroppenstedtiistrain.
Department of Clinical Microbiology, Complejo Asistencial Universitario de León, Spain; Institute of Biomedicine (IBIOMED), León, Spain.
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Fernández-Natal
Microbiology Section, Department of Biotechnology and Food Science, Faculty of Science, University of Burgos, Burgos, Spain.
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Rodríguez-Lázaro
Department of Clinical Microbiology, Complejo Asistencial Universitario de León, Spain.
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Marrodán-Ciordia
Bacterial Taxonomy Laboratory, Centro Nacional de Microbiología, Instituto de Salud Carlos III, Majadahonda, Spain.
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Sáez-Nieto
Bacterial Taxonomy Laboratory, Centro Nacional de Microbiología, Instituto de Salud Carlos III, Majadahonda, Spain.
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Valdezate
Department of Clinical Microbiology, Complejo Asistencial Universitario de León, Spain.
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Rodríguez-Pollán
Centrum für Biotechnologie (CeBiTec), Universität Bielefeld, Bielefeld, Germany.
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Tauch
Public Health, School of Physiotherapy ONCE, Madrid, Spain.
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Soriano
Antimicrobial susceptibility; Corynebacterium kroppenstedtii; MALDI-TOF MS; granulomatous mastitis; multidrug-resistant isolate