Report on Liver Cell Transplantation Using Human Fetal Liver Cells.
In an era of organ shortage, human fetuses donated after medically indicated abortion could be considered a potential liver donor for hepatic cell isolation. We investigated transplantation of fetal liver cells as a strategy to support liver functionality in end-stage liver disease. Here, we report our protocol of human fetal liver cells (hFLC) isolation in fetuses from 17 to 22 gestational weeks, and our clinical procedure of hFLC transplantation through the splenic artery.
Hepatology Unit, Department of Medicine, Mediterranean Institute for Transplantation and Advanced Specialized Therapies, IRCCS-ISMETT, Palermo, Italy. gpietrosi@ismett.edu.
Giada
G
Pietrosi
Fondazione Ri.MED, Regenerative Medicine and Biomedical Technologies Unit, Department of Laboratory Medicine and Advanced Biotechnologies, IRCCS-ISMETT, Palermo, Italy.
Cinzia
C
Chinnici
D018929:Cell Culture Techniques; D002469:Cell Separation / Q000295:instrumentation / Q000379:methods*; D017690:Cell Transplantation / Q000009:adverse effects / Q000941:ethics / Q000379:methods* / Q000592:standards; D058625:End Stage Liver Disease / Q000628:therapy*; D005260:Female; D016332:Fetal Tissue Transplantation / Q000009:adverse effects / Q000941:ethics / Q000379:methods* / Q000592:standards; D005333:Fetus / Q000166:cytology*; D006084:Graft Rejection / Q000517:prevention & control; D022781:Hepatocytes / Q000276:immunology / Q000382:microbiology / Q000637:transplantation*; D006801:Humans; D007165:Immunosuppression Therapy / Q000379:methods; D007166:Immunosuppressive Agents / Q000627:therapeutic use; D008099:Liver / Q000166:cytology; D010477:Perfusion / Q000295:instrumentation / Q000379:methods; D017410:Practice Guidelines as Topic; D011247:Pregnancy; D011786:Quality Control; D013157:Splenic Artery / Q000601:surgery; D016559:Tacrolimus / Q000627:therapeutic use; D014019:Tissue Donors; D020858:Tissue and Organ Harvesting / Q000941:ethics / Q000379:methods / Q000592:standards
D007166:Immunosuppressive Agents; D016559:Tacrolimus
Chronic liver disease; Collagenase perfusion; Fetal liver; Fetal stem cell transplantation; Liver cell transplantation; Liver progenitors; Liver stem cell isolation