Digital holographic microscopy in label-free analysis of cultured cells' response to photodynamic treatment.
A methodology providing noninvasive monitoring and evaluation of the effect of photodynamic treatment on live cells in vitro is presented. Variations in morphological characteristics of cells in the course and after treatment are recorded by means of digital holographic microscopy. High-precision measurements of phase shift gained by probe radiation in HeLa and human endometrial mesenchymal stem cell cultures demonstrate for the first time changes of their volume occurred in response to treatment.
A V
AV
Belashov
A A
AA
Zhikhoreva
T N
TN
Belyaeva
E S
ES
Kornilova
N V
NV
Petrov
A V
AV
Salova
I V
IV
Semenova
O S
OS
Vasyutinskii
D006367:HeLa Cells; D006696:Holography / Q000379:methods*; D006801:Humans; D059630:Mesenchymal Stem Cells