His-tag based in situ labelling of progeny viruses for real-time single virus tracking in living cells.
Tracking virus infection events in live cells is useful for understanding the mechanism of virus infection, and fluorescent labelling is a critical step. Herein a noninvasive strategy for labelling viruses with His-tags was developed by in situ modifying the cell surface proteins with polypeptides containing His-tags during progeny virus assembly. The His-tagged viruses were further conjugated with Ni2+-nitrilotriacetate complex modified quantum dots, and retained their infectivity for real-time single virus tracking in living cells.
Key Laboratory of Luminescent and Real-Time Analytical Chemistry (Southwest University), Ministry of Education, College of Pharmaceutical Sciences, Southwest University, Chongqing 400715, PR China. chengzhi@swu.edu.cn.
Lin Ling
LL
Zheng
Key Laboratory of Luminescent and Real-Time Analytical Chemistry (Southwest University), Ministry of Education, College of Pharmaceutical Sciences, Southwest University, Chongqing 400715, PR China. chengzhi@swu.edu.cn.
Chun Mei
CM
Li
Chongqing Key Laboratory of Biomedical Analysis (Southwest University), Chongqing Science & Technology Commission, College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, PR China.
Shu Jun
SJ
Zhen
Chongqing Key Laboratory of Biomedical Analysis (Southwest University), Chongqing Science & Technology Commission, College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, PR China.
Yuan Fang
YF
Li
Key Laboratory of Luminescent and Real-Time Analytical Chemistry (Southwest University), Ministry of Education, College of Pharmaceutical Sciences, Southwest University, Chongqing 400715, PR China. chengzhi@swu.edu.cn and Chongqing Key Laboratory of Biomedical Analysis (Southwest University), Chongqing Science & Technology Commission, College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, PR China.
Cheng Zhi
CZ
Huang
D056945:Hep G2 Cells; D006639:Histidine / Q000737:chemistry*; D006801:Humans; D007202:Indicators and Reagents; D010455:Peptides / Q000737:chemistry*; D045663:Quantum Dots; D019065:Virus Assembly; D014780:Viruses / Q000302:isolation & purification*
D007202:Indicators and Reagents; D010455:Peptides; D006639:Histidine