Tissue Engineering Platforms to Replicate the Tumor Microenvironment of Multiple Myeloma.
We described here the manufacturing and implementation of two prototype perfusion culture devices designed primarily for the cultivation of difficult-to-preserve primary patient-derived multiple myeloma cells (MMC). The first device consists of an osteoblast (OSB)-derived 3D tissue scaffold constructed in a perfused microfluidic environment. The second platform is a 96-well plate-modified perfusion culture device that can be utilized to reconstruct several tissue and tumor microenvironments utilizing both primary human and murine cells. This culture device was designed and fabricated specifically to: (1) enable the preservation of primary MMC for downstream use in biological studies and chemosensitivity analyses and, (2) provide a high-throughput format that is compatible with plate readers specifically seeing that this system is built on an industry standard 96-well tissue culture plate.
Department of Chemical Engineering and Materials Science, Stevens Institute of Technology, Hoboken, NJ, 07030, USA.
Wenting
W
Zhang
Department of Chemical Engineering and Materials Science, Stevens Institute of Technology, Hoboken, NJ, 07030, USA.
Woo Y
WY
Lee
John Theurer Cancer Center, Hackensack University Medical Center, 40 Prospect Avenue, Hackensack, NJ, 07601, USA. Jenny.Zilberberg@hackensackmeridian.org.
Jenny
J
Zilberberg
D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry / Q000494:pharmacology; D001854:Bone Marrow Cells / Q000187:drug effects / Q000473:pathology; D002460:Cell Line; D049109:Cell Proliferation; D003470:Culture Media / Q000494:pharmacology; D004129:Dimethylpolysiloxanes / Q000737:chemistry / Q000494:pharmacology; D004867:Equipment Design; D006801:Humans; D056656:Lab-On-A-Chip Devices; D051379:Mice; D009101:Multiple Myeloma / Q000473:pathology*; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects; D010477:Perfusion / Q000295:instrumentation / Q000379:methods; D057285:Precision Medicine / Q000295:instrumentation*; D061251:Primary Cell Culture; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds; D059016:Tumor Microenvironment / Q000502:physiology
D001672:Biocompatible Materials; D003470:Culture Media; D004129:Dimethylpolysiloxanes; C013830:baysilon
Microenvironment; Microfluidics; Multiple myeloma; Perfusion; Three-dimensional