Atypical variants of nodular lymphocyte-predominant Hodgkin lymphoma show low microvessel density and vessels of distention type.
Nodular lymphocyte-predominant Hodgkin lymphoma (NLPHL) presents different histopathologic growth patterns, including atypical forms showing overlapping histopathologic and clinical features with T-cell/histiocyte-rich large B-cell lymphoma (THRLBCL). Because growth patterns are associated with vessel distribution, the aim of the present study was to compare angiogenesis in different NLPHL patterns with THRLBCL as well as other lymphomas. Atypical variants of NLPHL and THRLBCL (n=10 per group) both showed a low microvessel density (MVD; 1.16-1.31/μm2) with a diffuse vessel distribution. In contrast, in typical NLPHL (n=10), follicular areas with low MVD were retained, whereas an increase in vessels in the interfollicular areas was observed (MVD 1.35/μm2). THRLBCL and typical NLPHL could additionally be distinguished by differences in their molecular angiogenesis signature. Furthermore, the number of intravascular T cells was significantly reduced in THRLBCL (0.0028 T cells/mm2vessel area) when compared with typical NLPHL (0.0059 T cells/mm2vessel area), potentially reflecting the different composition of the microenvironment in these 2 lymphoma entities. The results of our study reveal a similar vascular pattern and angiogenesis behavior in atypical NLPHL variants and THRLBCL in contrast to the retained follicular pattern in typical NLPHL.
Dr Senckenberg Institute of Pathology, Goethe University, 60590 Frankfurt am Main, Germany.
Victoria
V
Scheidt
Dr Senckenberg Institute of Pathology, Goethe University, 60590 Frankfurt am Main, Germany.
Martin-Leo
ML
Hansmann
Dr Senckenberg Institute of Pathology, Goethe University, 60590 Frankfurt am Main, Germany.
Bianca
B
Schuhmacher
Dr Senckenberg Institute of Pathology, Goethe University, 60590 Frankfurt am Main, Germany.
Claudia
C
Döring
Dr Senckenberg Institute of Pathology, Goethe University, 60590 Frankfurt am Main, Germany. Electronic address: s.hartmann@em.uni-frankfurt.de.
Sylvia
S
Hartmann
D014408:Biomarkers, Tumor / Q000032:analysis / Q000235:genetics; D001706:Biopsy; D003937:Diagnosis, Differential; D006689:Hodgkin Disease / Q000235:genetics / Q000276:immunology / Q000473:pathology*; D006801:Humans; D007150:Immunohistochemistry; D008198:Lymph Nodes / Q000276:immunology / Q000473:pathology*; D018655:Lymphocyte Count; D016393:Lymphoma, B-Cell / Q000235:genetics / Q000276:immunology / Q000473:pathology*; D055806:Microvessels / Q000473:pathology*; D009389:Neovascularization, Pathologic; D011237:Predictive Value of Tests; D013601:T-Lymphocytes / Q000276:immunology / Q000473:pathology; D059016:Tumor Microenvironment
D014408:Biomarkers, Tumor
Angiogenesis; Classical Hodgkin lymphoma; Microvessel density; Nodular lymphocyte–predominant Hodgkin lymphoma; T-cell/histiocyte-rich large B-cell lymphoma