Shrimp STAT was hijacked by white spot syndrome virus immediate-early protein IE1 involved in modulation of viral genes.
STATs are a family of transcription factors that regulate a cascade of cellular processes including cell growth, differentiation, apoptosis and immune responses. However, they are usually targeted by viruses to assist infection. In this study, we identified that white spot syndrome virus (WSSV) immediate-early protein IE1 interacted with Litopenaeus vannamei STAT (LvSTAT) and thereby led to its phosphorylation activation. In addition, we demonstrated that LvSTAT could bind to the promoters of the viral immediate-early genes wsv051 and ie1 through STAT-binding motifs in vitro and vivo, allowing the enhancement of their promoters' activities. Moreover, IE1 could promote the transcriptional activation activity of LvSTAT to augment the transcription of wsv051 and ie1. In conclusion, our findings revealed a novel linkage between WSSV IE1 and shrimp STAT, which was a clue to well understand how WSSV adopted the active strategies to modulate the shrimp signaling pathway.
School of Life Science, Xiamen University, Xiamen 361005, People's Republic of China; State Key Laboratory Breeding Base of Marine Genetic Resources, Key Laboratory of Marine Genetic Resources of State Oceanic Administration, Third Institute of Oceanography, State Oceanic Administration, Key Laboratory of Marine Genetic Resources of Fujian Province, South China Sea Bio-Resource Exploitation and Utilization Collaborative Innovation Center, Xiamen 361005, People's Republic of China.
Defu
D
Yao
State Key Laboratory Breeding Base of Marine Genetic Resources, Key Laboratory of Marine Genetic Resources of State Oceanic Administration, Third Institute of Oceanography, State Oceanic Administration, Key Laboratory of Marine Genetic Resources of Fujian Province, South China Sea Bio-Resource Exploitation and Utilization Collaborative Innovation Center, Xiamen 361005, People's Republic of China. Electronic address: ruanlingwei@tio.org.cn.
Lingwei
L
Ruan
State Key Laboratory Breeding Base of Marine Genetic Resources, Key Laboratory of Marine Genetic Resources of State Oceanic Administration, Third Institute of Oceanography, State Oceanic Administration, Key Laboratory of Marine Genetic Resources of Fujian Province, South China Sea Bio-Resource Exploitation and Utilization Collaborative Innovation Center, Xiamen 361005, People's Republic of China.
Huasong
H
Lu
State Key Laboratory Breeding Base of Marine Genetic Resources, Key Laboratory of Marine Genetic Resources of State Oceanic Administration, Third Institute of Oceanography, State Oceanic Administration, Key Laboratory of Marine Genetic Resources of Fujian Province, South China Sea Bio-Resource Exploitation and Utilization Collaborative Innovation Center, Xiamen 361005, People's Republic of China.
Hong
H
Shi
State Key Laboratory Breeding Base of Marine Genetic Resources, Key Laboratory of Marine Genetic Resources of State Oceanic Administration, Third Institute of Oceanography, State Oceanic Administration, Key Laboratory of Marine Genetic Resources of Fujian Province, South China Sea Bio-Resource Exploitation and Utilization Collaborative Innovation Center, Xiamen 361005, People's Republic of China.
Xun
X
Xu
D000818:Animals; D060829:Arthropod Proteins / Q000235:genetics* / Q000378:metabolism; D015967:Gene Expression Regulation, Viral; D017874:Immediate-Early Proteins / Q000235:genetics* / Q000378:metabolism; D033561:Penaeidae / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D010766:Phosphorylation; D050791:STAT Transcription Factors / Q000235:genetics* / Q000378:metabolism; D046848:White spot syndrome virus 1 / Q000235:genetics / Q000502:physiology*
D060829:Arthropod Proteins; D017874:Immediate-Early Proteins; D050791:STAT Transcription Factors
IE1; Interaction; STAT; Shrimp; Transcription regulation; WSSV