Synthesis of reticulated hollow spheres structure NiCo2S4and its application in organophosphate pesticides biosensor.
Electrode materials play a key role in the development of electrochemical sensors, particularly enzyme-based biosensors. Here, a novel NiCo2S4with reticulated hollow spheres assembled from rod-like structures was prepared by a one-pot solvothermal method and its formation mechanism was discussed. Moreover, comparison of NiCo2S4materials from different experiment conditions as biosensors was investigated by electrochemical impedance spectroscopy (EIS) and differential pulse voltammetry (DPV), and the best one that was reticulated hollow spheres assembled from rod-like structures NiCo2S4has been successfully employed as a matrix of AChE immobilization for the special structure, superior conductivity and rich reaction active sites. When using common two kinds of organophosphate pesticides (OPs) as model analyte, the biosensors demonstrated a wide linear range of 1.0×10-12-1.0×10-8gmL-1with the detection limit of 4.2×10-13gmL-1for methyl parathion, and 1.0×10-13-1.0×10-10gmL-1with the detection limit of 3.5×10-14gmL-1for paraoxon, respectively. The proposed biosensors exhibited many advantages such as acceptable stability and low cost, providing a promising tool for analysis of OPs.
School of Environmental and Municipal Engineering, Xi'an University of Architecture and Technology, Xi'an 710055, China.
Lei
L
Peng
School of Environmental and Municipal Engineering, Xi'an University of Architecture and Technology, Xi'an 710055, China; College of Sciences, Xi'an University of Architecture and Technology, Xi'an 710055, China. Electronic address: dongsyy@126.com.
Sheying
S
Dong
College of Sciences, Xi'an University of Architecture and Technology, Xi'an 710055, China.
Wenbo
W
Wei
College of Sciences, Xi'an University of Architecture and Technology, Xi'an 710055, China.
Xiaojing
X
Yuan
School of Environmental and Municipal Engineering, Xi'an University of Architecture and Technology, Xi'an 710055, China.
Tinglin
T
Huang
D000110:Acetylcholinesterase / Q000378:metabolism; D015374:Biosensing Techniques / Q000379:methods*; D002800:Cholinesterase Inhibitors / Q000032:analysis / Q000378:metabolism; D003035:Cobalt / Q000737:chemistry*; D055664:Electrochemical Techniques / Q000379:methods; D004800:Enzymes, Immobilized / Q000378:metabolism; D057230:Limit of Detection; D008743:Methyl Parathion / Q000032:analysis* / Q000378:metabolism; D009532:Nickel / Q000737:chemistry*; D010261:Paraoxon / Q000032:analysis* / Q000378:metabolism; D010575:Pesticides / Q000032:analysis* / Q000378:metabolism; D013440:Sulfides / Q000737:chemistry*
D002800:Cholinesterase Inhibitors; D004800:Enzymes, Immobilized; D010575:Pesticides; D013440:Sulfides; D003035:Cobalt; D008743:Methyl Parathion; D009532:Nickel; D000110:Acetylcholinesterase; D010261:Paraoxon
Acetylcholinesterase; NiCo(2)S(4); Organophosphate pesticides; Sensor; Synthesis