Purification Techniques for Three-Dimensional DNA Nanostructures.
Separation of self-assembled three-dimensional nanostructures from excess staple strands, misfolded structures, or unattached functional elements is critical for downstream applications. Numerous purification techniques exist, with varying yields, purities, and hetero-element compatibilities. In this chapter, we focus on three such techniques-agarose gel electrophoresis, ultrafiltration, and polymeric bead pull-down-which together satisfy requirements for a range of applications.
Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, GA, USA. travis.meyer@emory.edu.|Biomolecular Nanoengineering Lab, Emory School of Medicine, Health Sciences Research Bldg E186, 1760 Haygood Drive, Atlanta, GA, 30322, USA. travis.meyer@emory.edu.
Travis A
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Meyer
D004247:DNA / Q000737:chemistry*; D004587:Electrophoresis, Agar Gel / Q000379:methods; D049329:Nanostructures / Q000737:chemistry*; D036103:Nanotechnology / Q000379:methods; D009690:Nucleic Acid Conformation
D004247:DNA
Agarose gel electrophoresis; DNA nanotechnology; Polymeric bead pull-down; Purification; Three-dimensional DNA nanostructures; Ultrafiltration