A novel alkaloid from Portulaca oleracea L.
A novel alkaloid named oleraciamide C (1), with six known compounds, hydroxydihydrobovolide (2), uracil (3), catechol (4), 4-aminophenol (5), vanillic acid (6) as well as 3-hydroxypyridine (7), were isolated from Portulaca oleracea L. Additionally, hydroxydihydrobovolide (2), 4-aminophenol (5), 3-hydroxypyridine (7) were obtained from the plant for the first time. Structure of the new compound was determined using spectroscopic methods including HR-ESI-TOF-MS, 1D and 2D NMR. Others were elucidated through1H NMR,13C NMR spectra and comparison with literature data. Notably, Compound 1 possessed an unusual bis-substituted eight-membered ring linked with the β-glucopyranose moiety. The cytotoxicity of compound 1 was evaluated against human adipose-derived stem cells (hADSCs) by CCK-8 method.
a School of Pharmacy , Liaoning University of Traditional Chinese Medicine , Dalian , China.
Liang
L
Xu
b School of the First Clinic , Liaoning University of Traditional Chinese Medicine , Shenyang , China.
Zheming
Z
Ying
c Regenerative Medicine Center , The First Affiliated Hospital of Dalian Medical University , Dalian , China.
Wenjuan
W
Wei
a School of Pharmacy , Liaoning University of Traditional Chinese Medicine , Dalian , China.
Dong
D
Hao
a School of Pharmacy , Liaoning University of Traditional Chinese Medicine , Dalian , China.
Haibo
H
Wang
a School of Pharmacy , Liaoning University of Traditional Chinese Medicine , Dalian , China.
Wenjie
W
Zhang
a School of Pharmacy , Liaoning University of Traditional Chinese Medicine , Dalian , China.
Cuiyu
C
Li
a School of Pharmacy , Liaoning University of Traditional Chinese Medicine , Dalian , China.
Mingyue
M
Jiang
a School of Pharmacy , Liaoning University of Traditional Chinese Medicine , Dalian , China.
Xixiang
X
Ying
c Regenerative Medicine Center , The First Affiliated Hospital of Dalian Medical University , Dalian , China.
Jing
J
Liu
D000273:Adipose Tissue / Q000166:cytology; D000470:Alkaloids / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D000627:Aminophenols / Q000302:isolation & purification; D002301:Cardiac Glycosides / Q000737:chemistry; D002396:Catechols / Q000302:isolation & purification; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D004353:Drug Evaluation, Preclinical / Q000379:methods; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D009005:Monosaccharides / Q000737:chemistry* / Q000494:pharmacology; D010946:Plants, Medicinal / Q000737:chemistry; D031822:Portulaca / Q000737:chemistry*; D011725:Pyridines / Q000302:isolation & purification; D021241:Spectrometry, Mass, Electrospray Ionization; D013234:Stem Cells / Q000187:drug effects; D014641:Vanillic Acid / Q000302:isolation & purification
D000470:Alkaloids; D000627:Aminophenols; D002301:Cardiac Glycosides; D002396:Catechols; D009005:Monosaccharides; D011725:Pyridines; C000619392:hydroxydihydrobovolide; C000619391:oleraciamide C; C023500:3-hydroxypyridine; D014641:Vanillic Acid; C034221:catechol; C026729:4-aminophenol
NMR; Portulaca oleracea L.; alkaloid; cytotoxicity