ROS mediated pro-apoptotic effects ofTinospora cordifoliaon breast cancer cells.
The inevitable development of chemoresistance and unmanageable side effects are the major therapeutic challenges in management of breast cancer imposing an urgent need for identification of novel therapeutic agents. In the present investigation, we report anti-proliferative activity of chloroform fraction ofTinospora cordifolia(TcCF),an Ayurvedic medicinal plant, on breast cancer cells. We found that TcCF inhibited growth of breast cancer cells, MDA-MB-231 and MCF-7. More interestingly, we observed TcCF treatment increased intra-cellular ROS levels, altered expression of pro and anti-apoptotic genes, decreased colony formation ability and induced apoptosis in breast cancer cells. We also found that inhibition of ROS abrogated TcCF induced apoptosis in breast cancer cells, emphasizing the role ROS in TcCF induced breast cancer cell death. Furthermore, we identified the presence of pharmacologically active compounds like rutin and quercetin which account for the anti-cancer property of TcCF against breast cancer cells. These data show TcCF is a promising anti-cancer agent against breast cancer cells.
Molecular Cell Biology Lab, Department of Biochemistry, King George's Medical University, Lucknow 226003, UP, India, Department of Chemistry, Integral University, Lucknow 226 026, U.P., India.
Jamal Akhtar
JA
Ansari
Molecular Cell Biology Lab, Department of Biochemistry, King George's Medical University, Lucknow 226003, UP, India.
Namrata
N
Rastogi
Department of Biochemistry, King George's Medical University, Lucknow-226 003, Uttar Pradesh, India, kaleembaksh@gmail.com.
Mohammad Kaleem
MK
Ahmad
Molecular Cell Biology Lab, Department of Biochemistry, King George's Medical University, Lucknow 226003, UP, India.
Abbas Ali
AA
Mahdi
Department of Chemistry, Integral University, Lucknow 226 026, U.P., India.
Abdul Rahman
AR
Khan
Division of Endocrinology, Central Drug Research Institute, Lucknow 226 031, U.P., India.
Ravi
R
Thakur
Department of Chemistry, Integral University, Lucknow 226 026, U.P., India.
Vikas Kumar
VK
Srivastava
Division of Endocrinology, Central Drug Research Institute, Lucknow 226 031, U.P., India.
Durga Prasad
DP
Mishra
Molecular Cell Biology Lab, Department of Biochemistry, King George's Medical University, Lucknow 226003, UP, India, Department of Chemistry, Integral University, Lucknow 226 026, U.P., India.
Nishat
N
Fatima
Molecular Cell Biology Lab, Department of Biochemistry, King George's Medical University, Lucknow 226003, UP, India, Department of Chemistry, Integral University, Lucknow 226 026, U.P., India.
Homa Jilani
HJ
Khan
Molecular Cell Biology Lab, Department of Biochemistry, King George's Medical University, Lucknow 226003, UP, India.
Mohammad
M
Waseem
D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000494:pharmacology*; D017209:Apoptosis; D001943:Breast Neoplasms / Q000188:drug therapy*; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D059808:Polyphenols / Q000737:chemistry / Q000302:isolation & purification; D017382:Reactive Oxygen Species / Q000378:metabolism*; D031610:Tinospora / Q000737:chemistry*; D014407:Tumor Cells, Cultured
D000972:Antineoplastic Agents, Phytogenic; D010936:Plant Extracts; D059808:Polyphenols; D017382:Reactive Oxygen Species