Rapid Screening and Identification of BSA Bound Ligands from Radix astragali Using BSA Immobilized Magnetic Nanoparticles Coupled with HPLC-MS.
Radix astragaliis widely used either as a single herb or as a collection of herbs in a complex prescription in China. In this study, bovine serum albumin functionalized magnetic nanoparticles (BSA-MN) coupled with high performance liquid chromatography-mass spectrometry (HPLC-MS) were used to screen and identify bound ligands from then-butanol part of aRadix astragaliextract. The prepared BSA-MN showed sufficient magnetic response for the separation with an ordinary magnet and satisfied reusability. Fundamental parameters affecting the preparation of BSA-MN and the screening efficiency were studied and optimized. Under the optimum conditions, four bound ligands were screened out from then-butanol part of aRadix astragaliextract and identified as genistin (1), calycosin-7-O-β-d-glucoside (2), ononin (3) and formononetin (4). This effective method could be widely applied for rapid screening and identification of active compounds from complex mixtures without the need for preparative isolation.
Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, Changsha 410205, China. liuliangliang@caas.cn.
Liangliang
L
Liu
Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, Changsha 410205, China. juanlengcaas@yahoo.com.
Juan
J
Leng
Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, Changsha 410205, China. xiaiyang@yahoo.com.
Xiai
X
Yang
Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, Changsha 410205, China. lipingliaocaas@yahoo.com.
Liping
L
Liao
College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, China. hn_cenyin@csu.edu.cn.
Yin
Y
Cen
Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, Changsha 410205, China. aipingxiao@yahoo.com.
Aiping
A
Xiao
State Key Laboratory of Cotton Biology, Institute of Cotton Research of CAAS, Anyang 455000, China. malei@caas.cn.
Lei
L
Ma
D020001:1-Butanol / Q000737:chemistry / Q000302:isolation & purification; D027885:Astragalus propinquus; D002851:Chromatography, High Pressure Liquid; D004365:Drugs, Chinese Herbal / Q000737:chemistry* / Q000302:isolation & purification*; D005960:Glucosides / Q000737:chemistry / Q000302:isolation & purification; D007529:Isoflavones / Q000737:chemistry / Q000302:isolation & purification; D008024:Ligands; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D013058:Mass Spectrometry; D012710:Serum Albumin, Bovine / Q000737:chemistry
D004365:Drugs, Chinese Herbal; D005960:Glucosides; D007529:Isoflavones; D008024:Ligands; D058185:Magnetite Nanoparticles; C526426:calycosin-7-O-beta-D-glucoside; C040641:genistin; D012710:Serum Albumin, Bovine; C007768:formononetin; D020001:1-Butanol; C027492:Huang Qi
HPLC-MS; Radix astragali; bovine serum albumin; ligands; magnetic nanoparticles