PEG Grafted-Nanodiamonds for the Delivery of Gemcitabine.
Carboxyl end-functionalized poly[poly(ethylene glycol) methyl ether methacrylate] [P(PEGMEMA)] and its block copolymer with gemcitabine substituted poly(N-hydroxysuccinimide methacrylate) [PGem-block-P(PEGMEMA)] are synthesized via reversible addition-fragmentation transfer (RAFT) polymerization. Then, two polymers are grafted onto the surface of amine-functionalized nanodiamonds to obtain [P(PEGMEMA)]-grafted nanodiamonds (ND-PEG) and [PGem-block-P(PEGMEMA)]-grafted nanodiamonds (ND-PF). Gemcitabine is physically absorbed to ND-PEG to produce ND-PEG (Gem). Two polymer-grafted nanodiamonds (i.e., with physically absorbed gemcitabine ND-PEG (Gem) and with chemically conjugated gemcitabine ND-PF) are characterized using attenuated total reflectance infrared spectroscopy, dynamic light scattering, and thermogravimetric analysis. The drug release, cytotoxicity (to seed human pancreatic carcinoma AsPC-1 cells), and cellular uptake of ND-PEG (Gem) and ND-PF are also investigated.
Centre for Advanced Macromolecular Design, School of Chemistry, University of New South Wales, Sydney, NSW, 2052, Australia.
Mingxia
M
Lu
Centre for Advanced Macromolecular Design, School of Chemistry, University of New South Wales, Sydney, NSW, 2052, Australia.
Yu-Kai
YK
Wang
Centre for Advanced Macromolecular Design, School of Chemistry, University of New South Wales, Sydney, NSW, 2052, Australia.
Jiacheng
J
Zhao
Centre for Advanced Macromolecular Design, School of Chemistry, University of New South Wales, Sydney, NSW, 2052, Australia.
Hongxu
H
Lu
Centre for Advanced Macromolecular Design, School of Chemistry, University of New South Wales, Sydney, NSW, 2052, Australia.
Martina H
MH
Stenzel
Centre for Advanced Macromolecular Design, School of Chemistry, University of New South Wales, Sydney, NSW, 2052, Australia.
Pu
P
Xiao
D045744:Cell Line, Tumor; D003841:Deoxycytidine / Q000031:analogs & derivatives* / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology; D016503:Drug Delivery Systems / Q000379:methods*; D006801:Humans; D058612:Nanodiamonds / Q000737:chemistry*; D010190:Pancreatic Neoplasms / Q000188:drug therapy* / Q000378:metabolism; D011092:Polyethylene Glycols / Q000737:chemistry*; D000093542:Gemcitabine
D058612:Nanodiamonds; D003841:Deoxycytidine; D011092:Polyethylene Glycols; C000595209:polyethylene glycol 1000; D000093542:Gemcitabine
drug delivery; gemcitabine; nanodiamonds; pancreatic cancer