Myosin accelerates synaptic vesicle recycling in the motor nerve endings.
Neurotransmitter release and exocytosis of synaptic vesicles in the motor nerve endings of the frog cutaneous-pectoris muscle were studied using electrophysiological and optical methods under the conditions of inhibition of the myosin light-chain kinase and non-muscle myosin by the specific inhibitors ML-7 (12 μM) and (-)-blebbistatin (100 μM). At high-frequency stimulation (20 pulses/s), these inhibitors strengthened suppression of transmitter release during the first 20-25 s and slowed down the release of the fluorescent dye FM 1-43. The obtained results indicate that myosin accelerates rapid synaptic vesicle recycling upon high-frequency stimulation.
Kazan State Medical University, Ministry of Health, Kazan, Tatarstan, Russia.
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Grigoryev
Kazan State Medical University, Ministry of Health, Kazan, Tatarstan, Russia. zefiroval@rambler.ru.
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Zefirov
D000818:Animals; D066298:In Vitro Techniques; D009046:Motor Neurons / Q000502:physiology*; D009218:Myosins / Q000378:metabolism*; D009469:Neuromuscular Junction / Q000502:physiology*; D017729:Presynaptic Terminals / Q000502:physiology*; D011898:Ranidae; D009435:Synaptic Transmission / Q000502:physiology*; D013572:Synaptic Vesicles / Q000502:physiology*
D009218:Myosins