Effect of platelet-rich plasma on polypropylene meshes implanted in the rabbit vagina: histological analysis.
OBJECTIVE
The polypropylene mesh (PPM) is used in many surgical interventions because of its good incorporation and accessibility. However, potential mesh-related complications are common. Platelet-rich plasma (PRP) improves the healing of wounds and is inexpensive. Thus, the purpose of this study was to analyze the effect of the PRP-gel coating of a PPM on inflammation, production of collagen, and smooth muscle in the rabbit vagina.


METHODS
The intervention consisted of a 1.5cm incision and divulsion of the vaginal mucosa for the implantation of a PRP-coated PPM. The PRP-coated mesh was implanted in 15 rabbits, and in the second group, the same implant was used without the PRP coating. In the sham group, the intervention consisted of the incision, divulsion, and suture. The rabbits were euthanized at 7, 30 and 90 days, and full-thickness sagittal sections of the posterior vaginal wall and rectum were scored. The inflammatory infiltrate was evaluated using hematoxylin and eosin staining. The Sirius Red stain was used to examine deposition of collagen I and III, and Masson's trichrome staining was used to visualize the smooth muscle.


RESULTS
The group with PRP-coated meshes had a lower inflammatory infiltrate count at 30 days. Deposition of collagen III increased with the use of PRP-coating at 90 days.


CONCLUSIONS
The area of inflammatory infiltrate was significantly increased in the group without the PRP-coated mesh at 30 days but not in the group with the PRPcoated mesh, indicating a less intense inflammatory response. In addition, a significant increase in collagen III occurred at 90 days.
Departamento de Cirurgia, Universidade Estadual de Londrina, Londrina, PR, Brasil.
Natália Gomes
NG
Parizzi
Departamento de Cirurgia, Universidade do Oeste Paulista, Presidente Prudente, SP, Brasil.
Oscar Ávila
OÁ
Rubini
Departamento de Cirurgia, Universidade Estadual de Londrina, Londrina, PR, Brasil.
Silvio Henrique Maia de
SHM
Almeida
Departamento de Cirurgia, Universidade Estadual de Londrina, Londrina, PR, Brasil.
Lais Caetano
LC
Ireno
Departamento de Cirurgia, Universidade Estadual de Londrina, Londrina, PR, Brasil.
Roger Mitio
RM
Tashiro
Departamento de Cirurgia, Universidade Estadual de Londrina, Londrina, PR, Brasil.
Victor Hugo Tolotto de
VHT
Carvalho
D000818:Animals; D003094:Collagen / Q000378:metabolism; D005260:Female; D053657:Platelet-Rich Plasma; D011126:Polypropylenes; D011183:Postoperative Complications; D011817:Rabbits; D013526:Surgical Mesh / Q000009:adverse effects; D014621:Vagina / Q000473:pathology / Q000601:surgery*; D014945:Wound Healing
D011126:Polypropylenes; D003094:Collagen
Collagen; Inflammation ; Platelet-Rich Plasma