Microwaved bacterial cellulose-based hydrogel microparticles for the healing of partial thickness burn wounds.
Burn wound management is a complex process because the damage may extend as far as the dermis which has an acknowledged slow rate of regeneration. This study investigates the feasibility of using hydrogel microparticles composed of bacterial cellulose and polyacrylamide as a dressing material for coverage of partial-thickness burn wounds. The microparticulate carrier structure and surface morphology were investigated by Fourier transform infrared, X-ray diffraction, elemental analysis, and scanning electron microscopy. The cytotoxicity profile of the microparticles showed cytocompatibility with L929 cells. Dermal irritation test demonstrated that the hydrogel was non-irritant to the skin and had a significant effect on wound contraction compared to the untreated group. Moreover, histological examination of in vivo burn healing samples revealed that the hydrogel treatment enhanced epithelialization and accelerated fibroblast proliferation with wound repair and intact skin achieved by the end of the study. Both the in vitro and in vivo results proved the biocompatibility and efficacy of hydrogel microparticles as a wound dressing material.
School of Pharmacy, International Medical University, Kuala Lumpur, Malaysia.|Centre for Drug Delivery Research, Faculty of Pharmacy, Universiti Kebangsaan Malaysia, Jalan Raja Muda Abdul Aziz, 50300, Kuala Lumpur, Malaysia.
Manisha
M
Pandey
Centre for Drug Delivery Research, Faculty of Pharmacy, Universiti Kebangsaan Malaysia, Jalan Raja Muda Abdul Aziz, 50300, Kuala Lumpur, Malaysia.
Najwa
N
Mohamad
School of Pharmacy, Faculty of Science and Engineering, University of Wolverhampton, Wulfruna Street, Wolverhampton, WV1 1LY, UK.
Wan-Li
WL
Low
School of Pharmacy, Faculty of Science and Engineering, University of Wolverhampton, Wulfruna Street, Wolverhampton, WV1 1LY, UK.
Claire
C
Martin
Centre for Drug Delivery Research, Faculty of Pharmacy, Universiti Kebangsaan Malaysia, Jalan Raja Muda Abdul Aziz, 50300, Kuala Lumpur, Malaysia. mciamin@ukm.edu.my.
Mohd Cairul Iqbal
MC
Mohd Amin
D000180:Acrylic Resins / Q000008:administration & dosage / Q000737:chemistry / Q000528:radiation effects / Q000627:therapeutic use; D000818:Animals; D001419:Bacteria; D002056:Burns / Q000188:drug therapy / Q000473:pathology; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D002482:Cellulose / Q000008:administration & dosage / Q000737:chemistry / Q000528:radiation effects / Q000627:therapeutic use; D004337:Drug Carriers / Q000008:administration & dosage / Q000737:chemistry / Q000528:radiation effects / Q000627:therapeutic use; D005260:Female; D020100:Hydrogels / Q000008:administration & dosage / Q000737:chemistry / Q000528:radiation effects / Q000627:therapeutic use; D051379:Mice; D008872:Microwaves; D017207:Rats, Sprague-Dawley; D012867:Skin / Q000187:drug effects / Q000473:pathology; D023422:Skin Irritancy Tests; D014945:Wound Healing / Q000187:drug effects; D000099166:Polyacrylamides
D000180:Acrylic Resins; D002482:Cellulose; D004337:Drug Carriers; D020100:Hydrogels; D000099166:Polyacrylamides
Bacterial cellulose; Cytotoxicity; Hydrogel microparticles; Microwave irradiation; Partial-thickness burn wound