Temperature-dependent regulation of gene expression in Japanese flounder Paralichthys olivaceus kidney after Edwardsiella tarda formalin-killed cells.
Temperature affects the activities of the immune system and the susceptibility of fish to pathogens. To investigate the modulation of temperature on immune related gene expression in formalin-killed cells (FKC) of Edwardsiella tarda-injected Japanese flounder Paralichthys olivaceus, fish reared at 15 or 22 °C were injected with FKC of E. tarda. The up-regulation of immune related genes was detected in FKC-injected fish at both temperatures by qPCR. The mRNA expression of IFNγ was highly up-regulated at 6 h post injection (hpi) in FKC-injected fish at 15 °C, whereas at 22 °C, strong up-regulation of the gene was detected at 3 hpi The mRNA expression level of IRF1 was detected from 3 hpi to day 14 post injection in fish reared at 15 °C, but the gene was up-regulated from 3 to 6 hpi in fish reared at 22 °C. Comprehensive gene expression profiling showed that immune related genes are differentially expressed between 15 and 22 °C. Genes involved in the IFNγ signaling pathway were up-regulated at 22 °C but not at 15 °C. These results demonstrate that gene(s) involved in IFNγ signaling pathway in Japanese flounder stimulated with FKC of E. tarda are regulated by temperature.
Laboratory of Genome Science, Graduate School of Tokyo University of Marine Science and Technology, Konan 4-5-7, Minato-ku, Tokyo 108-8477, Japan.
Norie
N
Kaneshige
Laboratory of Genome Science, Graduate School of Tokyo University of Marine Science and Technology, Konan 4-5-7, Minato-ku, Tokyo 108-8477, Japan.
Walissara
W
Jirapongpairoj
Laboratory of Genome Science, Graduate School of Tokyo University of Marine Science and Technology, Konan 4-5-7, Minato-ku, Tokyo 108-8477, Japan.
Ikuo
I
Hirono
Laboratory of Genome Science, Graduate School of Tokyo University of Marine Science and Technology, Konan 4-5-7, Minato-ku, Tokyo 108-8477, Japan. Electronic address: h-kondo@kaiyodai.ac.jp.
Hidehiro
H
Kondo
D000818:Animals; D016207:Cytokines / Q000235:genetics* / Q000378:metabolism; D020609:Edwardsiella tarda / Q000276:immunology*; D029941:Fish Proteins / Q000235:genetics* / Q000378:metabolism; D005412:Flatfishes / Q000235:genetics* / Q000276:immunology; D005557:Formaldehyde; D005786:Gene Expression Regulation; D006358:Hot Temperature / Q000009:adverse effects*; D007113:Immunity, Innate; D007274:Injections, Intraperitoneal / Q000662:veterinary; D007668:Kidney / Q000276:immunology; D020411:Oligonucleotide Array Sequence Analysis / Q000662:veterinary
D016207:Cytokines; D029941:Fish Proteins; D005557:Formaldehyde
Edwardsiella tarda; Japanese flounder; Microarray; Temperature