Cellular Model of p21-Induced Senescence.
Cellular senescence is a unique process of normal physiology, from embryonic development to aging, also known for its association with a broad range of pathological conditions. Therefore a reliable model of cellular senescence remains an indispensable tool for the investigation of senescence-associated changes and human disease. Here we describe a model of HT1080 fibrosarcoma cells with an inducible senescence phenotype. These cells are equipped with the lac repressor and exogenous p21 under the control of a lac repressor regulated promoter. The senescent phenotype is induced in these cells by isopropyl-β-D-thiogalactopyranoside (IPTG)-inducible expression of senescence-associated cell cycle inhibitor p21Waf1/Cip1/Sdi1.
Department of Drug Discovery and Biomedical Sciences, South Carolina College of Pharmacy, University of South Carolina, Columbia, SC, USA.
Michael
M
Shtutman
PeptiMed, Inc., Madison, WI, USA.
Bey-Dih
BD
Chang
Department of Drug Discovery and Biomedical Sciences, South Carolina College of Pharmacy, University of South Carolina, Columbia, SC, USA.
Gary P
GP
Schools
Department of Drug Discovery and Biomedical Sciences, South Carolina College of Pharmacy, University of South Carolina, Columbia, SC, USA. broude@sccp.sc.edu.
Eugenia V
EV
Broude
D015415:Biomarkers; D002453:Cell Cycle / Q000235:genetics; D002460:Cell Line; D016922:Cellular Senescence / Q000502:physiology*; D050759:Cyclin-Dependent Kinase Inhibitor p21 / Q000235:genetics* / Q000378:metabolism*; D005434:Flow Cytometry; D015870:Gene Expression; D055879:Gene Knock-In Techniques; D006801:Humans; D010641:Phenotype
D015415:Biomarkers; D050759:Cyclin-Dependent Kinase Inhibitor p21
HT1080-p21-9; IPTG; Lac-repressor; Senescence; p21Waf1/Cip1/Sdi1