Versatility of a localized surface plasmon resonance-based gold nanoparticle-alloyed quantum dot nanobiosensor for immunofluorescence detection of viruses.
Flu infection, caused by the influenza virus, constitutes a serious threat to human lives worldwide. A rapid, sensitive and specific diagnosis is urgently needed for point-of-care treatment and to control the rapid spread of this disease. In this study, an ultrasensitive, rapid and specific localized surface plasmon resonance (LSPR)-induced immunofluorescence nanobiosensor has been developed for the influenza virus based on a gold nanoparticle (AuNP)-induced quantum dot (QD) fluorescence signal. Alloyed quaternary CdSeTeS QDs were synthesized via the hot-injection organometallic route and were subsequently capped with l-cysteine via a ligand exchange reaction. AuNPs were synthesized in HEPES buffer and thiolated with l-cysteine. The concept of the biosensor involves the conjugation of anti-neuraminidase (NA) antibody (anti-NA Ab) to thiolated AuNPs and the conjugation of anti-hemagglutinin (HA) antibody (anti-HA Ab) to alloyed quaternary l-cysteine-capped CdSeTeS QDs. Interaction of the antigens displaying on the surface of the influenza virus target with anti-NA Ab-conjugated AuNPs and anti-HA Ab-conjugated QDs induces an LSPR signal from adjacent AuNPs to trigger fluorescence-enhancement changes in the QDs in proportion to the concentration of the target virus. The detection limit for influenza H1N1 virus was 0.03pg/mL in deionized water and 0.4pg/mL in human serum; while, for the clinically isolated H3N2, the detection limit was 10PFU/mL. The detection of influenza virus H1N1 was accomplished with high sensitivity. The versatility of the biosensor was demonstrated for the detection of clinically isolated influenza virus H3N2 and norovirus-like particles (NoV-LPs).
Laboratory of Biotechnology, Department of Agriculture, Graduate School of Integrated Science and Technology, Shizuoka University, 836 Ohya, Suruga-ku, Shizuoka 422-8529, Japan. Electronic address: kenpi901@yahoo.co.jp.
Kenshin
K
Takemura
Laboratory of Biotechnology, Research Institute of Green Science and Technology, Shizuoka University, 836 Ohya, Suruga-ku, Shizuoka 422-8529, Japan. Electronic address: adegoke.sesan@mailbox.co.za.
Oluwasesan
O
Adegoke
Laboratory of Biotechnology, Department of Agriculture, Graduate School of Integrated Science and Technology, Shizuoka University, 836 Ohya, Suruga-ku, Shizuoka 422-8529, Japan. Electronic address: pakkun0449@yahoo.co.jp.
Naoto
N
Takahashi
Laboratory of Biotechnology, Research Institute of Green Science and Technology, Shizuoka University, 836 Ohya, Suruga-ku, Shizuoka 422-8529, Japan; College of Agriculture, Academic Institute, Shizuoka University, 836 Ohya, Suruga-ku, Shizuoka 422-8529, Japan. Electronic address: kato.tatsuya@shizuoka.ac.jp.
Tatsuya
T
Kato
Department of Virology II, National Institute of Infectious Diseases, Gakuen 4-7-1, Musashi-Murayama, Tokyo 208-0011, Japan. Electronic address: litc@nih.go.jp.
Tian-Cheng
TC
Li
School of Human Science and Environment, University of Hyogo, 1-1-12 Shinzaike-Honcho, Himeji, Hyogo 670-0092, Japan. Electronic address: kitamoto@shse.u-hyogo.ac.jp.
Noritoshi
N
Kitamoto
Hidaka General Hospital, Gobo, Wakayama 644-0002, Japan. Electronic address: moqui-tom@maia.eonet.ne.jp.
Tomoyuki
T
Tanaka
Department of Infectious Diseases, Hamamatsu University School of Medicine, 1-20-1 Higashi-ku, Handa-yama, Hamamatsu 431-3192, Japan. Electronic address: tesuzuki@hama-med.ac.jp.
Tetsuro
T
Suzuki
Laboratory of Biotechnology, Research Institute of Green Science and Technology, Shizuoka University, 836 Ohya, Suruga-ku, Shizuoka 422-8529, Japan; College of Agriculture, Academic Institute, Shizuoka University, 836 Ohya, Suruga-ku, Shizuoka 422-8529, Japan. Electronic address: park.enoch@shizuoka.ac.jp.
Enoch Y
EY
Park
D015374:Biosensing Techniques; D003545:Cysteine / Q000737:chemistry; D006046:Gold / Q000737:chemistry; D019267:Hemagglutinin Glycoproteins, Influenza Virus / Q000302:isolation & purification*; D006801:Humans; D053118:Influenza A Virus, H1N1 Subtype / Q000737:chemistry / Q000302:isolation & purification*; D053122:Influenza A Virus, H3N2 Subtype / Q000737:chemistry / Q000302:isolation & purification*; D007251:Influenza, Human / Q000175:diagnosis* / Q000821:virology; D057230:Limit of Detection; D053768:Metal Nanoparticles / Q000737:chemistry; D045663:Quantum Dots / Q000737:chemistry; D020349:Surface Plasmon Resonance
D019267:Hemagglutinin Glycoproteins, Influenza Virus; D006046:Gold; D003545:Cysteine
Alloyed quantum dot; Gold nanoparticle; Immunofluorescence detection; Influenza virus; Localized surface plasmon resonance; Norovirus