Improving respiration measurements with gas exchange analyzers.
Dark respiration measurements with open-flow gas exchange analyzers are often questioned for their low accuracy as their low values often reach the precision limit of the instrument. Respiration was measured in five species, two hypostomatous (Vitis Vinifera L. and Acanthus mollis) and three amphistomatous, one with similar amount of stomata in both sides (Eucalyptus citriodora) and two with different stomata density (Brassica oleracea and Vicia faba). CO2differential (ΔCO2) increased two-fold with no change in apparent Rd, when the two leaves with higher stomatal density faced outside. These results showed a clear effect of the position of stomata on ΔCO2. Therefore, it can be concluded that leaf position is important to guarantee the improvement of respiration measurements increasing ΔCO2without affecting the respiration results by leaf or mass units. This method will help to increase the accuracy of leaf respiration measurements using gas exchange analyzers.
Institut de Recerca i Formació Agrària i Pesquera, Conselleria d'Agricultura, Medi Ambient i Territori. Govern de les Illes Balears, C/Eusebio Estada n° 145, 07009 Palma de Mallorca, Spain.
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Montero
Grup de Recerca en Biologia de les Plantes en Condicions Mediterrànies, Departament de Biologia, Universitat de les Illes Balears, Carretera de Valldemossa, km 7.5, 07122, Palma de Mallorca, Balears, Spain.
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Ribas-Carbó
Grup de Recerca en Biologia de les Plantes en Condicions Mediterrànies, Departament de Biologia, Universitat de les Illes Balears, Carretera de Valldemossa, km 7.5, 07122, Palma de Mallorca, Balears, Spain.
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Del Saz
Grup de Recerca en Biologia de les Plantes en Condicions Mediterrànies, Departament de Biologia, Universitat de les Illes Balears, Carretera de Valldemossa, km 7.5, 07122, Palma de Mallorca, Balears, Spain.
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El Aou-Ouad
Department of Global Ecology, Carnegie Institution for Science, Stanford, CA 94305, USA.
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Berry
Grup de Recerca en Biologia de les Plantes en Condicions Mediterrànies, Departament de Biologia, Universitat de les Illes Balears, Carretera de Valldemossa, km 7.5, 07122, Palma de Mallorca, Balears, Spain.
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Flexas
Grup de Recerca en Biologia de les Plantes en Condicions Mediterrànies, Departament de Biologia, Universitat de les Illes Balears, Carretera de Valldemossa, km 7.5, 07122, Palma de Mallorca, Balears, Spain. Electronic address: j.bota@uib.es.
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Bota
D019069:Cell Respiration; D003624:Darkness; D005740:Gases / Q000378:metabolism*; D010827:Physiology / Q000295:instrumentation* / Q000379:methods*; D054046:Plant Stomata / Q000166:cytology / Q000502:physiology; D010944:Plants / Q000378:metabolism
D005740:Gases
Amphistomatous leaf; Dark respiration; Gas exchange analyzer; Hypostomatous leaf; Overlapping leaves