In vitro and in vivo anti-coagulant activity and toxicological studies of marine sulfated glycosaminoglycans.
The present study aimed to characterize and evaluate the in vitro and in vivo anticoagulant activity of sulfated glycosaminoglycans from the skins of smooth hound (SHSG) and grey triggerfish (GTSG). The analysis of SHSG and GTSG with acetate cellulose electrophoresis in Zn-acetate revealed the presence of hyaluronic acid (HA), chondroitin sulfate (CS) and dermatan sulfate (DS). Both glycosaminoglycans were evaluated for their in vitro anticoagulant activities using activated partial thromboplastin time (aPTT), thrombin time (TT) and prothrombine time (PT) tests. SHSG and GTSG and calciparin were tested as in vivo anticoagulants by subcutaneous (s.c) injection to adult female Wistar rats in a concentration of 75mg/kg of body weight. The administration of SHSG, GTSG and calciparin to rats induced a significant decrease of platelet rates compared to the control. The aPTT assay of SHSG and GTSG was prolonged 1.3 and 1.23-fold respectively compared with the control. Toxicity studies were performed to investigate whether or not SHSG and GTSG can cause pathological changes in the liver, proteins and DNA. The concentration and catalytic activity of liver oxidative stress markers and enzymes, respectively, as well as the observed hepatic morphological changes indicated that calciparin induced hepatic toxicity and oxidative damage in the liver. The higher activity and lower toxicity of SHSG and GTSG recommended these compounds as a better drug candidate than calciparin.
Laboratoire d'Amélioration des Plantes et Valorisation des Agro-Ressources, ENIS, Université de Sfax, 3038 Tunisia.
Fatma
F
Krichen
Unité de Recherche Pharmacologie et Toxicologie des Xénobiotiques, Faculté de Médecine de Sfax, Sfax, 3029, Tunisia.
Zohra
Z
Ghlissi
Centre Régional de Transfusion Sanguine de Sfax, Route El-Ain Km 0.5, CP 3003 Sfax, Tunisia.
Ikram Ben
IB
Amor
Laboratoire d'Amélioration des Plantes et Valorisation des Agro-Ressources, ENIS, Université de Sfax, 3038 Tunisia.
Nadhem
N
Sayari
Laboratoire Anatomie Pathologie, CHU Habib Bourguiba, Sfax 3029, Tunisia.
Rim
R
Kallel
Centre Régional de Transfusion Sanguine de Sfax, Route El-Ain Km 0.5, CP 3003 Sfax, Tunisia.
Jalel
J
Gargouri
Unité de Recherche Pharmacologie et Toxicologie des Xénobiotiques, Faculté de Médecine de Sfax, Sfax, 3029, Tunisia.
Zouheir
Z
Sahnoun
Laboratoire Anatomie Pathologie, CHU Habib Bourguiba, Sfax 3029, Tunisia.
Tahia
T
Boudawara
Laboratoire d'Amélioration des Plantes et Valorisation des Agro-Ressources, ENIS, Université de Sfax, 3038 Tunisia. Electronic address: ali.bougatef79@gmail.com.
Ali
A
Bougatef
D000818:Animals; D000925:Anticoagulants / Q000737:chemistry / Q000494:pharmacology*; D005260:Female; D005399:Fishes; D006025:Glycosaminoglycans / Q000737:chemistry / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D012867:Skin / Q000737:chemistry
D000925:Anticoagulants; D006025:Glycosaminoglycans
Anticoagulant activity; Fish skins; Sulfated glycosaminoglycans; Toxicological studies