Novel insights into the regulatory roles of gene hshB in Xanthomonas oryzae pv. oryzicola.
Xanthomonas oryzae pv. oryzicola causes leaf streak disease of rice. The gene hshB is a newly identified virulence-associated gene that is co-regulated by diffusible signal factor signaling and global regulator Clp in X. oryzae pv. oryzicola. Our previous study showed that mutation of hshB remarkably impaired the virulence, extracellular protease activity, extracellular polysaccharide production and resistance to oxidative stress of X. oryzae pv. oryzicola. In this study, the regulatory role of hshB in X. oryzae pv .oryzicola was expanded. Results showed that hshB was also required for cell swimming motility. Transcriptome analysis showed that 305 genes were significantly differentially expressed after deletion of hshB in X. oryzae pv. oryzicola. Further analysis of transcriptome data indicated that the differentially expressed genes focused on two aspects: namely, cell motility and cell signal transduction. This finding strongly identified the closely related function of hshB to cell motility and signal transduction. In addition, the mutation of hshB of X. oryzae pv. oryzicola enhanced biofilm formation. Collectively, the study showed novel functions of gene hshB in cell motility and biofilm formation by transcriptome analysis, thus expanding our understanding of the roles of gene hshB in the pathogenic X. oryzae pv. oryzicola.
Institute of Plant Protection, Jiangsu Academy of Agricultural Science, Nanjing 210014, China.
Zhiwei
Z
Song
Institute of Plant Protection, Jiangsu Academy of Agricultural Science, Nanjing 210014, China.
Yancun
Y
Zhao
College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China; Key Laboratory of Integrated Management of Crop Diseases and Pests (Nanjing Agricultural University), Ministry of Education, China.
Guoliang
G
Qian
College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China; Key Laboratory of Integrated Management of Crop Diseases and Pests (Nanjing Agricultural University), Ministry of Education, China.
Benard Omondi
BO
Odhiambo
Institute of Plant Protection, Jiangsu Academy of Agricultural Science, Nanjing 210014, China. Electronic address: fqliu20011@sina.com.
Fengquan
F
Liu
D000581:Amidohydrolases / Q000235:genetics* / Q000378:metabolism; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D018441:Biofilms / Q000254:growth & development; D004269:DNA, Bacterial; D017353:Gene Deletion; D020869:Gene Expression Profiling; D015964:Gene Expression Regulation, Bacterial; D009068:Movement; D009154:Mutation; D012275:Oryza / Q000382:microbiology; D010935:Plant Diseases / Q000382:microbiology; D053038:Quorum Sensing; D015398:Signal Transduction; D014774:Virulence / Q000235:genetics*; D014974:Xanthomonas / Q000235:genetics* / Q000378:metabolism / Q000472:pathogenicity*
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D000581:Amidohydrolases; C587551:hshB protein, Xanthomonas oryzae
Biofilm formation; Cell motility; Quorum sensing; Xanthomonas oryzae pv. oryzicola; hshB