Regulation of the Docosapentaenoic Acid/Docosahexaenoic Acid Ratio (DPA/DHA Ratio) in Schizochytrium limacinum B4D1.
Docosapentaenoic acid/docosahexaenoic acid ratio (DPA/DHA ratio) in Schizochytrium was relatively stable. But ideally the ratio of DPA/DHA will vary according to the desired end use. This study reports several ways of modulating the DPA/DHA ratio. Incubation times changed the DPA/DHA ratio, and changes in this ratio were associated with the variations in the saturated fatty acid (SFAs) content. Propionic acid sharply increased the SFAs content in lipids, dramatically decreased the even-chain SFAs content, and reduced the DPA/DHA ratio. Pentanoic acid (C5:0) and heptanoic acid (C7:0) had similar effects as propionic acid, whereas butyric acid (C4:0), hexanoic acid (C6:0), and octanoic acid (C8:0) did not change the fatty acid profile and the DPA/DHA ratio. Transcription analyses show that β-oxidation might be responsible for this phenomenon. Iodoacetamide upregulated polyunsaturated fatty acid (PUFA) synthase genes, reduced the DHA content, and improved the DPA content, causing the DPA/DHA ratio to increase. These results present new insights into the regulation of the DPA/DHA ratio.
Tianjin Key Laboratory for Industrial BioSystems and Bioprocessing Engineering, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, 300308, China.
Ke
K
Zhang
Tianjin Central Hospital of Gynecology and Obstetrics, Tianjin, 300100, China.
Huidong
H
Li
Tianjin Key Laboratory for Industrial BioSystems and Bioprocessing Engineering, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, 300308, China.
Wuxi
W
Chen
Tianjin Key Laboratory for Industrial BioSystems and Bioprocessing Engineering, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, 300308, China.
Minli
M
Zhao
Tianjin Key Laboratory for Industrial BioSystems and Bioprocessing Engineering, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, 300308, China.
Haiyang
H
Cui
Tianjin Key Laboratory for Industrial BioSystems and Bioprocessing Engineering, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, 300308, China.
Qingsong
Q
Min
Tianjin Key Laboratory for Industrial BioSystems and Bioprocessing Engineering, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, 300308, China.
Haijun
H
Wang
Tianjin Key Laboratory for Industrial BioSystems and Bioprocessing Engineering, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, 300308, China.
Shulin
S
Chen
Tianjin Key Laboratory for Industrial BioSystems and Bioprocessing Engineering, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, 300308, China. li_dm@tib.cas.cn.
Demao
D
Li
D020148:Butyric Acid / Q000378:metabolism / Q000494:pharmacology; D002208:Caproates / Q000378:metabolism / Q000494:pharmacology; D002210:Caprylates / Q000378:metabolism / Q000494:pharmacology; D004281:Docosahexaenoic Acids / Q000096:biosynthesis*; D064429:Fatty Acid Synthases / Q000235:genetics* / Q000378:metabolism; D005227:Fatty Acids / Q000096:biosynthesis; D005231:Fatty Acids, Unsaturated / Q000096:biosynthesis*; D006538:Heptanoic Acids / Q000378:metabolism / Q000494:pharmacology; D007460:Iodoacetamide / Q000494:pharmacology; D010421:Pentanoic Acids / Q000378:metabolism / Q000494:pharmacology; D011422:Propionates / Q000378:metabolism / Q000494:pharmacology; D058009:Stramenopiles / Q000187:drug effects* / Q000201:enzymology / Q000235:genetics / Q000254:growth & development; D014158:Transcription, Genetic / Q000187:drug effects*
D002208:Caproates; D002210:Caprylates; D005227:Fatty Acids; D005231:Fatty Acids, Unsaturated; D006538:Heptanoic Acids; D010421:Pentanoic Acids; D011422:Propionates; D020148:Butyric Acid; C037652:hexanoic acid; D004281:Docosahexaenoic Acids; D064429:Fatty Acid Synthases; C029658:propionic acid; C026219:docosapentaenoic acid; C031492:octanoic acid; D007460:Iodoacetamide
Docosapentaenoic acid; Docosapentaenoic acid / docosahexaenoic acid ratio; Docosapentaenoic acid biosynthesis; Polyunsaturated fatty acid synthase; Schizochytrium