Antioxidant and anti-inflammatory action of melatonin in an experimental model of secondary biliary cirrhosis induced by bile duct ligation.
OBJECTIVE
To evaluate the effects of melatonin (Mel) on oxidative stress in an experimental model of bile duct ligation (BDL).


METHODS
Male Wistar rats (n= 32, weight ± 300 g) were allocated across four groups: CO (sham BDL), BDL (BDL surgery), CO + Mel (sham BDL and Mel administration) and BDL + Mel (BDL surgery and Mel administration). Mel was administered intraperitoneally for 2 wk, starting on postoperative day 15, at a dose of 20 mg/kg.


RESULTS
Mel was effective at the different standards, reestablishing normal liver enzyme levels, reducing the hepatosomatic and splenosomatic indices, restoring lipoperoxidation and antioxidant enzyme concentrations, reducing fibrosis and inflammation, and thereby reducing liver tissue injury in the treated animals.


CONCLUSIONS
The results of this study suggest a protective effect of Mel when administered to rats with secondary biliary cirrhosis induced by BDL.
Josieli Raskopf Colares, Elizângela Gonçalves Schemitt, Renata Minuzzo Hartmann, Adriane Dal Bosco, Graduate Program in Medical Sciences, Universidade Federal do Rio Grande do Sul, Porto Alegre 90040-060, Brazil.
Josieli Raskopf
JR
Colares
Josieli Raskopf Colares, Elizângela Gonçalves Schemitt, Renata Minuzzo Hartmann, Adriane Dal Bosco, Graduate Program in Medical Sciences, Universidade Federal do Rio Grande do Sul, Porto Alegre 90040-060, Brazil.
Elizângela Gonçalves
EG
Schemitt
Josieli Raskopf Colares, Elizângela Gonçalves Schemitt, Renata Minuzzo Hartmann, Adriane Dal Bosco, Graduate Program in Medical Sciences, Universidade Federal do Rio Grande do Sul, Porto Alegre 90040-060, Brazil.
Renata Minuzzo
RM
Hartmann
Josieli Raskopf Colares, Elizângela Gonçalves Schemitt, Renata Minuzzo Hartmann, Adriane Dal Bosco, Graduate Program in Medical Sciences, Universidade Federal do Rio Grande do Sul, Porto Alegre 90040-060, Brazil.
Francielli
F
Licks
Josieli Raskopf Colares, Elizângela Gonçalves Schemitt, Renata Minuzzo Hartmann, Adriane Dal Bosco, Graduate Program in Medical Sciences, Universidade Federal do Rio Grande do Sul, Porto Alegre 90040-060, Brazil.
Mariana do Couto
MD
Soares
Josieli Raskopf Colares, Elizângela Gonçalves Schemitt, Renata Minuzzo Hartmann, Adriane Dal Bosco, Graduate Program in Medical Sciences, Universidade Federal do Rio Grande do Sul, Porto Alegre 90040-060, Brazil.
Adriane Dal
AD
Bosco
Josieli Raskopf Colares, Elizângela Gonçalves Schemitt, Renata Minuzzo Hartmann, Adriane Dal Bosco, Graduate Program in Medical Sciences, Universidade Federal do Rio Grande do Sul, Porto Alegre 90040-060, Brazil.
Norma Possa
NP
Marroni
D000818:Animals; D000975:Antioxidants / Q000378:metabolism / Q000494:pharmacology / Q000627:therapeutic use*; D004195:Disease Models, Animal; D005978:Glutathione / Q000378:metabolism; D015227:Lipid Peroxidation; D008099:Liver / Q000187:drug effects / Q000201:enzymology / Q000473:pathology; D008105:Liver Cirrhosis, Biliary / Q000188:drug therapy* / Q000209:etiology / Q000378:metabolism; D008297:Male; D008550:Melatonin / Q000494:pharmacology / Q000627:therapeutic use*; D052247:Nitric Oxide Synthase Type II / Q000378:metabolism; D017208:Rats, Wistar; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
D000975:Antioxidants; D014409:Tumor Necrosis Factor-alpha; D052247:Nitric Oxide Synthase Type II; C496318:Nos2 protein, rat; D005978:Glutathione; D008550:Melatonin
Antioxidant; Cirrhosis; Fibrosis; Melatonin; Oxidative stress