MYB75 Phosphorylation by MPK4 Is Required for Light-Induced Anthocyanin Accumulation in Arabidopsis.
Light is a major environmental cue affecting various physiological and metabolic processes in plants. Although plant photoreceptors are well characterized, the mechanisms by which light regulates downstream responses are less clear. In Arabidopsis thaliana, the accumulation of photoprotective anthocyanin pigments is light dependent, and the R2R3 MYB transcription factor MYB75/PAP1 regulates anthocyanin accumulation. Here, we report that MYB75 interacts with and is phosphorylated by MAP KINASE4 (MPK4). Their interaction is dependent on MPK4 kinase activity and is required for full function of MYB75. MPK4 can be activated in response to light and is involved in the light-induced accumulation of anthocyanins. We show that MPK4 phosphorylation of MYB75 increases its stability and is essential for light-induced anthocyanin accumulation. Our findings reveal an important role for a MAPK pathway in light signal transduction.
State Key Laboratory of Plant Genomics, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China.|University of Chinese Academy of Sciences, Beijing 100049, China.
Shengnan
S
Li
State Key Laboratory of Plant Genomics, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China.|University of Chinese Academy of Sciences, Beijing 100049, China.
Wenyi
W
Wang
State Key Laboratory of Plant Genomics, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China.
Jinlan
J
Gao
State Key Laboratory of Plant Genomics, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China.
Kangquan
K
Yin
State Key Laboratory of Plant Genomics, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China.
Rui
R
Wang
State Key Laboratory of Plant Genomics, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China.
Chengcheng
C
Wang
Department of Biology, University of Copenhagen, 2200 Copenhagen N, Denmark.
Morten
0000-0002-3035-5991
M
Petersen
Department of Biology, University of Copenhagen, 2200 Copenhagen N, Denmark.
John
J
Mundy
State Key Laboratory of Plant Genomics, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China qiujl@im.ac.cn.
Jin-Long
0000-0001-8054-2552
JL
Qiu
D000872:Anthocyanins / Q000378:metabolism*; D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism* / Q000528:radiation effects; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D018506:Gene Expression Regulation, Plant / Q000235:genetics / Q000528:radiation effects; D008027:Light; D020928:Mitogen-Activated Protein Kinases / Q000235:genetics / Q000378:metabolism*; D000073718:Pancreatitis-Associated Proteins; D010766:Phosphorylation; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*
D000872:Anthocyanins; D029681:Arabidopsis Proteins; D020928:Mitogen-Activated Protein Kinases; D000073718:Pancreatitis-Associated Proteins; D014157:Transcription Factors; C502612:AT1G56650 protein, Arabidopsis; C000612141:REG3A protein, human; C436292:AtMPK4 protein, Arabidopsis