miRNA profiling in the mice in response to Echinococcus multilocularis infection.
miRNAs are small non-coding regulatory RNAs and actively contribute to the pathogenesis of parasitic diseases in multiple ways. The influence of Echinococcus multilocularis infection on host miRNAs remains unclear. Herein, it was shown that E. multilocularis infection disturbed the expression of 4 of 10 genes essential to miRNA biogenesis in the mouse liver, including ago1, ago4, tarbp2 and xrn2. Comparative analysis of deep sequencing data identified 46 differentially expressed miRNAs with 93.5% (43/46) being down-regulated, some of which are associated with modulation of liver cell death and fibrosis, and GO analysis revealed that these miRNAs were mainly enriched in signal transduction (p<0.008). Moreover, 57 miRNAs were commonly found to be edited in complex patterns in both control and E. multilocularis-infected samples. In some miRNAs, editing of nucleotides at the same or/and distinct positions in a given miRNA occurred in different frequencies. Correlation analysis showed that the mutation and editing rates of 57 commonly edited miRNAs were significantly correlated between both samples (r=0.9974, p<0.0001), suggesting little effect of E. multilocularis infection on miRNA mutation and editing. These results provide a rich and informative data for further studies of a role of host miRNAs during E. multilocularis infection.
Department of Ophthalmology, Shanghai Ninth People's Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, China.
Xiaoliang
X
Jin
State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, CAAS, Lanzhou 730046, China.
Xiaola
X
Guo
Department of Ophthalmology, Shanghai Ninth People's Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, China.
Dongqing
D
Zhu
Department of Pathobiology, Faculty of Veterinary Sciences, Bahauddin Zakariya University, Multan 60800, Pakistan.
Mazhar
M
Ayaz
State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, CAAS, Lanzhou 730046, China; Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou 225009, China. Electronic address: zhengyadong@caas.cn.
Yadong
Y
Zheng
D000818:Animals; D004443:Echinococcosis / Q000469:parasitology*; D048210:Echinococcus multilocularis / Q000235:genetics*; D005786:Gene Expression Regulation; D008099:Liver / Q000469:parasitology; D051379:Mice; D035683:MicroRNAs / Q000235:genetics*
D035683:MicroRNAs
Echinococcus multilocularis; Mutation and editing; miRNA