Biological conversion of the aqueous wastes from hydrothermal liquefaction of algae and pine wood by Rhodococci.
In this study, R. opacus PD630, R. jostii RHA1, R. jostii RHA1 VanA-, and their co-culture were employed to convert hydrothermal liquefaction aqueous waste (HTLAW) into lipids. After 11days, the COD reduction of algal-HTLAW reached 93.4% and 92.7% by R. jostii RHA1 and its mutant VanA-, respectively. Woody-HTLAW promoted lipid accumulation of 0.43glipid/gcell dry weight in R. opacus PD630 cells. Additionally, the total number of chemicals in HTLAW decreased by over 1/3 after 7days of coculture, and 0.10g/L and 0.46g/L lipids were incrementally accumulated in the cellular mass during the fermentation of wood- and algal-HTLAW, respectively. The GC-MS data supported that different metabolism pathways were followed when these Rhodococci strains degraded algae- and woody-HTLAW. These results indicated promising potential of bioconversion of under-utilized carbon and toxic compounds in HTLAW into useful products by selected Rhodococci.
Bioproducts, Sciences and Engineering Laboratory and Department of Biological Systems Engineering, Washington State University, Richland, WA 99354, United States.
Yucai
Y
He
Bioproducts, Sciences and Engineering Laboratory and Department of Biological Systems Engineering, Washington State University, Richland, WA 99354, United States.
Xiaolu
X
Li
Bioproducts, Sciences and Engineering Laboratory and Department of Biological Systems Engineering, Washington State University, Richland, WA 99354, United States.
Xiaoyun
X
Xue
Energy and Efficiency Division and the Bioproduct Sciences and Engineering Laboratory, Pacific Northwest National Laboratory, Richland, WA 99354, United States.
Marie S
MS
Swita
Energy and Efficiency Division and the Bioproduct Sciences and Engineering Laboratory, Pacific Northwest National Laboratory, Richland, WA 99354, United States.
Andrew J
AJ
Schmidt
Bioproducts, Sciences and Engineering Laboratory and Department of Biological Systems Engineering, Washington State University, Richland, WA 99354, United States. Electronic address: binyang@tricity.wsu.edu.
Bin
B
Yang
D002244:Carbon / Q000378:metabolism; D000460:Chlorophyta / Q000378:metabolism*; D005285:Fermentation; D008401:Gas Chromatography-Mass Spectrometry; D008055:Lipids; D028223:Pinus / Q000378:metabolism; D012240:Rhodococcus / Q000378:metabolism*; D018505:Waste Management / Q000379:methods; D014867:Water / Q000378:metabolism; D014934:Wood / Q000378:metabolism*
D008055:Lipids; D014867:Water; D002244:Carbon
Bioconversion; Coculture; Hydrothermal liquefaction aqueous waste; Lipid; Oleaginous Rhodococci