Enhanced production of poly(3-hydroxybutyrate-co-4-hydroxybutyrate) copolymer and antimicrobial yellow pigmentation from Cupriavidus sp. USMAHM13 with antibiofilm capability.
Antibiofilm polymers have the ability to inhibit bacterial biofilm formation, which is known to occur ubiquitously in the environment and pose risks of infection. In this study, production of P(3HB-co-4HB) copolymer and antimicrobial yellow pigment from Cupriavidus sp. USMAHM13 are enhanced through medium optimization. Before the improvement of yellow pigment production, screening for the best additional supplement was performed resulting in high-yield yellow pigmentation using yeast extract with optimum concentration of 2 g/L. Effects of different concentrations of 1,4-butanediol, ammonium acetate, and yeast extract were studied using central composite design. Under optimal conditions, 53 wt% of polyhydroxyalkanoate (PHA) content, 0.35 g/L of pigment concentration, and 5.87 g/L of residual biomass were achieved at 0.56 wt% C of 1,4-butanediol, 1.14 g/L of ammonium acetate, and 2 g/L of yeast extract. Antibiofilm tests revealed that the yellow pigment coated on P(3HB-co-4HB) copolymer had significant effect on the inhibition of bacteria proliferation and colonization from 6 hr onward reaching 100% inhibition by 12 hr, hence effectively inhibiting the biofilm formation.
a School of Biological Sciences , Universiti Sains Malaysia , Penang , Malaysia.
Iszatty
I
Ismail
a School of Biological Sciences , Universiti Sains Malaysia , Penang , Malaysia.
Tana Poorani
TP
Gurusamy
b School of Biological Sciences, Quest International University Perak , Perak , Malaysia.
Hema
H
Ramachandran
a School of Biological Sciences , Universiti Sains Malaysia , Penang , Malaysia.|c Malaysian Institute of Pharmaceuticals and Nutraceuticals, NIBM , Penang , Malaysia.|d Centre for Chemical Biology , Universiti Sains Malaysia , Penang , Malaysia.
Abdullah
A
Al-Ashraf Amirul
D000900:Anti-Bacterial Agents / Q000378:metabolism* / Q000494:pharmacology; D001412:Bacillus subtilis / Q000187:drug effects / Q000502:physiology; D018441:Biofilms / Q000187:drug effects; D049921:Cupriavidus / Q000378:metabolism*; D006885:Hydroxybutyrates / Q000378:metabolism* / Q000494:pharmacology; D007218:Industrial Microbiology / Q000379:methods*; D010860:Pigments, Biological / Q000378:metabolism* / Q000494:pharmacology; D011091:Polyesters / Q000378:metabolism* / Q000494:pharmacology; D000094122:Polyhydroxybutyrates
D000900:Anti-Bacterial Agents; D006885:Hydroxybutyrates; D010860:Pigments, Biological; D011091:Polyesters; C068056:poly(3-hydroxybutyrate-co-4-hydroxybutyrate); D000094122:Polyhydroxybutyrates
Antibiofilm; Cupriavidus sp. USMAHM13; P(3HB-co-4HB) copolymer; pigment-coated scaffold; yellow pigment