Qualitative Parameters of the Colonic Flora in Patients with HNF1A-MODY Are Different from Those Observed in Type 2 Diabetes Mellitus.
Background. Type 2 diabetes mellitus (T2DM) is determined by genetic and environmental factors. There have been many studies on the relationship between the composition of the gastrointestinal bacterial flora, T2DM, and obesity. There are no data, however, on the gut microbiome structure in monogenic forms of the disease including Maturity Onset Diabetes of the Young (MODY).Methods. The aim of the investigation was to compare the qualitative parameters of the colonic flora in patients with HNF1A-MODY and T2DM and healthy individuals. 16S sequencing of bacterial DNA isolated from the collected fecal samples using the MiSeq platform was performed.Results. There were significant between-group differences in the bacterial profile. At the phylum level, the amount of Proteobacteria was higher (p= 0.0006) and the amount of Bacteroidetes was lower (p= 0.0005) in T2DM group in comparison to the control group. In HNF1A-MODY group, the frequency of Bacteroidetes was lower than in the control group (p= 0.0143). At the order level, Turicibacterales was more abundant in HNF1A-MODY group than in T2DM group.Conclusions. It appears that there are differences in the gut microbiome composition between patients with HNF1A-MODY and type 2 diabetes. Further investigation on this matter should be conducted.
Department of Metabolic Diseases, Jagiellonian University Medical College, 15 Kopernika Street, 31-501 Kraków, Poland; University Hospital, 36 Kopernika Street, 31-501 Kraków, Poland.
Sandra
S
Mrozinska
Center for Medical Genomics OMICRON, Jagiellonian University Medical College, 7c Kopernika Street, 31-034 Kraków, Poland.
Piotr
P
Radkowski
Department of Microbiology, Jagiellonian University Medical College, 18 Czysta Street, 31-121 Kraków, Poland.
Tomasz
T
Gosiewski
Department of Metabolic Diseases, Jagiellonian University Medical College, 15 Kopernika Street, 31-501 Kraków, Poland; University Hospital, 36 Kopernika Street, 31-501 Kraków, Poland.
Magdalena
M
Szopa
Department of Microbiology, Jagiellonian University Medical College, 18 Czysta Street, 31-121 Kraków, Poland.
Malgorzata
M
Bulanda
Department of Metabolic Diseases, Jagiellonian University Medical College, 15 Kopernika Street, 31-501 Kraków, Poland; Center for Medical Genomics OMICRON, Jagiellonian University Medical College, 7c Kopernika Street, 31-034 Kraków, Poland.
Agnieszka H
AH
Ludwig-Galezowska
Department of Metabolic Diseases, Jagiellonian University Medical College, 15 Kopernika Street, 31-501 Kraków, Poland; University Hospital, 36 Kopernika Street, 31-501 Kraków, Poland.
Iwona
I
Morawska
Department of Microbiology, Jagiellonian University Medical College, 18 Czysta Street, 31-121 Kraków, Poland.
Agnieszka
A
Sroka-Oleksiak
Department of Metabolic Diseases, Jagiellonian University Medical College, 15 Kopernika Street, 31-501 Kraków, Poland.
Bartlomiej
B
Matejko
Center for Medical Genomics OMICRON, Jagiellonian University Medical College, 7c Kopernika Street, 31-034 Kraków, Poland.
Przemyslaw
P
Kapusta
Department of Microbiology, Jagiellonian University Medical College, 18 Czysta Street, 31-121 Kraków, Poland.
Dominika
D
Salamon
Department of Metabolic Diseases, Jagiellonian University Medical College, 15 Kopernika Street, 31-501 Kraków, Poland; University Hospital, 36 Kopernika Street, 31-501 Kraków, Poland.
Maciej T
MT
Malecki
Center for Medical Genomics OMICRON, Jagiellonian University Medical College, 7c Kopernika Street, 31-034 Kraków, Poland.
Pawel
P
Wolkow
Department of Metabolic Diseases, Jagiellonian University Medical College, 15 Kopernika Street, 31-501 Kraków, Poland; University Hospital, 36 Kopernika Street, 31-501 Kraków, Poland.
Tomasz
0000-0002-7199-4079
T
Klupa
D000328:Adult; D015373:Bacterial Typing Techniques; D041963:Bacteroidetes / Q000235:genetics / Q000302:isolation & purification; D016022:Case-Control Studies; D003106:Colon / Q000382:microbiology*; D003924:Diabetes Mellitus, Type 2 / Q000382:microbiology*; D005260:Female; D000069196:Gastrointestinal Microbiome / Q000235:genetics*; D006801:Humans; D008297:Male; D008875:Middle Aged; D010802:Phylogeny; D020560:Proteobacteria / Q000235:genetics / Q000302:isolation & purification; D012336:RNA, Ribosomal, 16S / Q000235:genetics
D012336:RNA, Ribosomal, 16S