Self-assembling peptide nanofibers containing phenylalanine for the controlled release of 5-fluorouracil.
The study shows that RADA-F6 peptide with pH-responsive self-assembling nature can be effectively used as a drug delivery system for the sustained release of a potent anticancer drug 5-fluorouracil (5-FU) at basic pH. As 5-FU contains the aromatic pyrimidine ring, RADA-F6 system is suitable for entrapping an aromatic drug due to effective π-π stacking with phenylalanine and be able to show better controlled release behavior. The stability and controlled release nature of RADA-F6 in different conditions followed by 5-FU entrapment at in silico conditions was confirmed by molecular dynamics simulation taking RADA-16 as control. Cytotoxicity of the drug-loaded RADA-F6 was measured by MTT assay and cellular uptake by confocal microscopy. Physicochemical characterization and further Western blot analysis and flow cytometric studies confirm that RADA-F6 can be successfully used as an efficient vector for pH-sensitive, controlled 5-FU delivery system.
Chemical Biology, Nano Drug Delivery Systems, Bio-Innovation Center.
Narayanan
N
Ashwanikumar
Cancer Research Programme.
Nisha Asok
NA
Kumar
Cancer Research Programme.
Padma S
PS
Saneesh Babu
Distributed Information Sub-Centre (Bioinformatics Centre), Rajiv Gandhi Centre for Biotechnology, Poojappura, Thiruvananthapuram, Kerala, India.
Krishnankutty C
KC
Sivakumar
Chemical Biology, Nano Drug Delivery Systems, Bio-Innovation Center.
Mithun Varghese
MV
Vadakkan
Chemical Biology, Nano Drug Delivery Systems, Bio-Innovation Center.
Parvathi
P
Nair
Chemical Biology, Nano Drug Delivery Systems, Bio-Innovation Center.
Ilamathi
I
Hema Saranya
Cancer Research Programme.
Sivakumari
S
Asha Nair
Chemical Biology, Nano Drug Delivery Systems, Bio-Innovation Center.
Gopalakrishnapillai S
GS
Vinod Kumar
D000964:Antimetabolites, Antineoplastic / Q000008:administration & dosage; D017209:Apoptosis; D002942:Circular Dichroism; D003692:Delayed-Action Preparations / Q000737:chemistry*; D004337:Drug Carriers / Q000737:chemistry*; D016503:Drug Delivery Systems; D005472:Fluorouracil / Q000008:administration & dosage*; D045325:HCT116 Cells; D006801:Humans; D020100:Hydrogels / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D018613:Microscopy, Confocal; D046529:Microscopy, Electron, Transmission; D056004:Molecular Dynamics Simulation; D057139:Nanofibers / Q000737:chemistry*; D050997:Nanomedicine / Q000379:methods; D010455:Peptides / Q000737:chemistry; D010649:Phenylalanine / Q000737:chemistry*; D013050:Spectrometry, Fluorescence
D000964:Antimetabolites, Antineoplastic; D003692:Delayed-Action Preparations; D004337:Drug Carriers; D020100:Hydrogels; D010455:Peptides; D010649:Phenylalanine; D005472:Fluorouracil
aromatic; drug delivery; nanofibrous; scaffold