A high accuracy cantilever array sensor for early liver cancer diagnosis.
Early liver cancer diagnosis has clinical significance in treating cancer. Joint detection of multiple biomarkers has been considered as an effective and reliable method for early cancer diagnosis. In this work, a novel biosensor based on microcantilever array was batch-fabricated for multiple liver cancer biomarkers detection with high sensitivity, high accuracy, high throughput, and high specification. A micro-cavity was designed in the free end of the cantilever for local reaction between antibody and antigen, which can dramatically reduce the effect of adsorption-induced stiffness coefficient k variation. Furthermore,the pillar arrays in the micro-cavity were designed for increasing detection upper limit. A linear relationship between the relative frequency shift and the antigen concentration was observed for three liver cancer biomarkers, alpha-fetoprotein (AFP), γ-glutamyltranspeptidase II (GGT-2), and hepatocyte growth factor (HGF). Slight cross-reaction response to different antigens ensures high specificity of the sensor. These features will promote clinical application of the cantilever sensors in early cancer diagnosis.
Institute of Semiconductors, Chinese Academy of Sciences, Qinghua Donglu A 35, Beijing, 100083, People's Republic of China.|School of Electronics and Information Engineering, Tianjin Polytechnic University, Tianjin, 300387, People's Republic of China.|State Key Laboratory of Transducer Technology, Shanghai, 200050, People's Republic of China.
Jingjing
J
Wang
Institute of Semiconductors, Chinese Academy of Sciences, Qinghua Donglu A 35, Beijing, 100083, People's Republic of China.|State Key Laboratory of Transducer Technology, Shanghai, 200050, People's Republic of China.
Lihao
L
Wang
Institute of Semiconductors, Chinese Academy of Sciences, Qinghua Donglu A 35, Beijing, 100083, People's Republic of China.|State Key Laboratory of Transducer Technology, Shanghai, 200050, People's Republic of China.
Yinfang
Y
Zhu
Institute of Semiconductors, Chinese Academy of Sciences, Qinghua Donglu A 35, Beijing, 100083, People's Republic of China.|State Key Laboratory of Transducer Technology, Shanghai, 200050, People's Republic of China.|University of Chinese Academy of Sciences, Beijing, 100083, People's Republic of China.
Jinying
J
Zhang
Institute of Semiconductors, Chinese Academy of Sciences, Qinghua Donglu A 35, Beijing, 100083, People's Republic of China.|State Key Laboratory of Transducer Technology, Shanghai, 200050, People's Republic of China.
Ju
J
Liao
Institute of Semiconductors, Chinese Academy of Sciences, Qinghua Donglu A 35, Beijing, 100083, People's Republic of China.|State Key Laboratory of Transducer Technology, Shanghai, 200050, People's Republic of China.
Shuaipeng
S
Wang
Institute of Semiconductors, Chinese Academy of Sciences, Qinghua Donglu A 35, Beijing, 100083, People's Republic of China. jlyang@semi.ac.cn.|State Key Laboratory of Transducer Technology, Shanghai, 200050, People's Republic of China. jlyang@semi.ac.cn.|University of Chinese Academy of Sciences, Beijing, 100083, People's Republic of China. jlyang@semi.ac.cn.
Jinling
J
Yang
Institute of Semiconductors, Chinese Academy of Sciences, Qinghua Donglu A 35, Beijing, 100083, People's Republic of China.|University of Chinese Academy of Sciences, Beijing, 100083, People's Republic of China.
Fuhua
F
Yang
D000951:Antigens, Neoplasm / Q000276:immunology; D014408:Biomarkers, Tumor / Q000378:metabolism; D003429:Cross Reactions; D055088:Early Detection of Cancer / Q000295:instrumentation*; D006801:Humans; D008113:Liver Neoplasms / Q000097:blood / Q000175:diagnosis*; D055616:Microtechnology; D012680:Sensitivity and Specificity
D000951:Antigens, Neoplasm; D014408:Biomarkers, Tumor
Liver cancer; MEMS cantilever; Mass detection; Multiple biomarkers; Stiffness coefficient