Association of Mannose-binding Lectin Polymorphisms with Tuberculosis Susceptibility among Chinese.
Tuberculosis (TB) is caused by infection of Mycobacterium tuberculosis. Host genetic variability is an important determinant of the risk of developing TB in humans. Although the association between MBL2 polymorphisms and TB has been studied in various populations, the results are controversial. In this study four functional single-nucleotide polymorphisms (SNPs, H/L, X/Y, P/Q and A/B) across the MBL2 gene were genotyped by direct DNA sequencing of PCR products in a case-control population of Chinese Han origin, consisting of 1,020 patients with pulmonary TB and 1,020 controls. We found that individuals carrying variant allele at A/B (namely BB or AB genotypes) was associated with increased susceptibility to TB (odds ratios [OR] = 1.57, 95% confidence interval [CI] 1.30-1.91, P = 1.3 × 10-6). Additionally, LYPB haplotype showed a significant association with increased risk of TB (OR = 1.54, 95% CI 1.27-1.87, P = 4.2 × 10-6; global haplotype association P = 3.5 × 10-5). Furthermore, individuals bearing low- or medium- MBL expression haplotype pairs had an increased risk of TB (OR = 1.56, 95% CI 1.29-1.90, P = 1.4 × 10-6). Thus, the reduced expression of functional MBL secondary to having MBL2 variants may partially mediate the increased susceptibility to TB risk.
Medical Experimental Center, General Hospital of Chinese People's Armed Police Forces, Beijing, P. R. China.|Department of Orthopedics (307 Hospital of PLA), Affiliated Hospital of Academy of Military Medical Sciences, Beijing, P. R. China.
Cheng
C
Liu
Beijing Institute of Biotechnology, Beijing, P. R. China.
Tao
T
He
Laboratory of Environment and Health, College of Life Sciences, University of Chinese Academy of Sciences, Beijing, P. R. China.|Department of Cardiology, General Hospital of Chinese People's Armed Police Forces, Beijing, P. R. China.|Department of Tuberculosis, The Fifth Hospital of Shijiazhuang, Shijiazhuang City, P. R. China.
Yanxiao
Y
Rong
Laboratory of Environment and Health, College of Life Sciences, University of Chinese Academy of Sciences, Beijing, P. R. China.|Department of Tuberculosis, The Fifth Hospital of Shijiazhuang, Shijiazhuang City, P. R. China.|Beijing Key Laboratory for Drug Resistance Tuberculosis Research, Beijing Chest Hospital, Capital Medical University, Beijing Tuberculosis and Thoracic Tumor Research Institute, Beijing, P. R. China.
Fengjiao
F
Du
Department of Cardiology, General Hospital of Chinese People's Armed Police Forces, Beijing, P. R. China.
Dongxing
D
Ma
Department of Cardiology, General Hospital of Chinese People's Armed Police Forces, Beijing, P. R. China.
Yujie
Y
Wei
Department of Tuberculosis, The Fifth Hospital of Shijiazhuang, Shijiazhuang City, P. R. China.
Zhiqin
Z
Mei
Department of Tuberculosis, The Fifth Hospital of Shijiazhuang, Shijiazhuang City, P. R. China.
Yuling
Y
Wang
Department of Tuberculosis, The Fifth Hospital of Shijiazhuang, Shijiazhuang City, P. R. China.
Haibin
H
Wang
Laboratory of Environment and Health, College of Life Sciences, University of Chinese Academy of Sciences, Beijing, P. R. China.|Department of Tuberculosis, The Fifth Hospital of Shijiazhuang, Shijiazhuang City, P. R. China.
Yuehua
Y
Zhu
Laboratory of Environment and Health, College of Life Sciences, University of Chinese Academy of Sciences, Beijing, P. R. China.|Department of Tuberculosis, The Fifth Hospital of Shijiazhuang, Shijiazhuang City, P. R. China.|Beijing Key Laboratory for Drug Resistance Tuberculosis Research, Beijing Chest Hospital, Capital Medical University, Beijing Tuberculosis and Thoracic Tumor Research Institute, Beijing, P. R. China.
Zongde
Z
Zhang
Medical Experimental Center, General Hospital of Chinese People's Armed Police Forces, Beijing, P. R. China.|Laboratory of Environment and Health, College of Life Sciences, University of Chinese Academy of Sciences, Beijing, P. R. China.|Department of Cardiology, General Hospital of Chinese People's Armed Police Forces, Beijing, P. R. China.
Li
L
Zheng
Army Tuberculosis Prevention and Control Key Laboratory, Institute for Tuberculosis Research, the 309th Hospital of Chinese PLA, Beijing, P. R. China.
Xueqiong
X
Wu
Medical Experimental Center, General Hospital of Chinese People's Armed Police Forces, Beijing, P. R. China.|Department of Cardiology, General Hospital of Chinese People's Armed Police Forces, Beijing, P. R. China.
Huiliang
H
Liu
Laboratory of Environment and Health, College of Life Sciences, University of Chinese Academy of Sciences, Beijing, P. R. China.
Wenjun
W
Ding
D000328:Adult; D044466:Asian People / Q000235:genetics*; D016022:Case-Control Studies; D005260:Female; D020022:Genetic Predisposition to Disease / Q000235:genetics*; D006239:Haplotypes / Q000235:genetics; D006801:Humans; D008297:Male; D037601:Mannose-Binding Lectin / Q000235:genetics*; D009169:Mycobacterium tuberculosis / Q000235:genetics; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D014397:Tuberculosis, Pulmonary / Q000235:genetics*
C445619:MBL2 protein, human; D037601:Mannose-Binding Lectin