Efficient approach to enhance drug solubility by particle engineering of bovine serum albumin.
The aim of this study was to investigate the use of bovine serum albumin (BSA) as a solubility enhancer for indometacin (IND) as a model drug. IND-BSA solid dispersions were prepared by both spray drying and freeze drying techniques using IND:BSA solution (20:1 Molar Ratio (MR)) and IND:BSA suspension (100:1 MR). The solid state of IND in solid dispersions was characterised by SEM, DSC and XRD. The aqueous solubility of IND in the presence of increased amounts of BSA was evaluated. Additionally, IND dissolution and release profiles were evaluated. IND in solid dispersions with BSA showed significantly higher solubility in water than that of the physical mixture of both. Enhancement factors of 24,000 and 100,000 were obtained for the solid dispersion formulated in 20:1 MR and 100:1 MR, respectively. Dissolution studies in-vitro indicated a significant increase in the dissolution rate of IND from solid dispersions compared to that of the free drug, with almost 95% of the drug dissolved in the first 5min. Furthermore, an immediate release of IND from BSA solid dispersions was shown. The potential use of albumin as solubility enhancer for poorly soluble drugs, particularly, for immediate release volume-limited dosage forms is reported.
Drug Discovery, Delivery and Patient Care (DDDPC) Theme, School of Life Sciences, Pharmacy and Chemistry, Kingston University London, Kingston Upon Thames, London, United Kingdom. Electronic address: m.khoder@kingston.ac.uk.
Mouhamad
M
Khoder
Drug Discovery, Delivery and Patient Care (DDDPC) Theme, School of Life Sciences, Pharmacy and Chemistry, Kingston University London, Kingston Upon Thames, London, United Kingdom; Department of Pharmaceutics, Faculty of Pharmacy, Minia University, Minia, Egypt.
Hamdy
H
Abdelkader
Drug Discovery, Delivery and Patient Care (DDDPC) Theme, School of Life Sciences, Pharmacy and Chemistry, Kingston University London, Kingston Upon Thames, London, United Kingdom.
Amr
A
ElShaer
Institute of Chemistry of Poitiers, Materials and Natural Resources, Catalysis and Unconventional Media's Team, University of Poitiers, France.
Ayman
A
Karam
Faculty of Medical Science, Anglia Ruskin University, Bishops Hall Lane, Chelmsford CM1 1SQ, United Kingdom.
Mohammad
M
Najlah
Drug Discovery, Delivery and Patient Care (DDDPC) Theme, School of Life Sciences, Pharmacy and Chemistry, Kingston University London, Kingston Upon Thames, London, United Kingdom; School of Pharmacy, The University of Auckland, Auckland, New Zealand.
Raid G
RG
Alany
D002626:Chemistry, Pharmaceutical / Q000379:methods; D004337:Drug Carriers / Q000737:chemistry; D005612:Freeze Drying / Q000379:methods; D007213:Indomethacin / Q000737:chemistry*; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D012995:Solubility; D014867:Water / Q000378:metabolism
D004337:Drug Carriers; D014867:Water; D012710:Serum Albumin, Bovine; D007213:Indomethacin
Bovine serum albumin; Dissolution rate; Indometacin; Solid dispersion; Solubility enhancer