Resveratrol and Coumarin: Novel Agricultural Antibacterial Agent against Ralstonia solanacearum In Vitro and In Vivo.
Bacterial wilt is a destructive disease caused by the phytopathogenRalstonia solanacearum(R. solanacearum), which is widely found in various tobacco-growing areas all over the world. Botanical bactericidal substances have gradually emerged as a hot topic in modern pesticide research. In this study, the antibacterial activities of two phytochemicals (resveratrol and coumarin) againstR. solanacearumand their in vivo and in vitro efficacy for controlling tobacco bacterial wilt were evaluated. We rule out significant biological effects of both phytochemicals using transmission electron microscope (TEM) and fluorescence microscope, which suppressed the growth ofR. solanacearum. Furthermore, we demonstrated that the toxicity mechanisms mainly involved damaging bacterial cell membrane and preventing swarming motility and biofilm formation. A further pot experiment demonstrated that coumarin and resveratrol significantly inhibited early adhesion and colonization ofR. solanacearumin tobacco plants and the corresponding control efficacies were 68% and 85% after incubation for 13 days, respectively. The findings of this study suggest that both resveratrol and coumarin have potential as non-toxic antimicrobial strategies for controlling tobacco bacterial wilt disease.
Laboratory of Natural Product Pesticide, College of Plant Protection, Southwest University, Chongqing 400715, China. chenhuanni521@126.com.
Juanni
J
Chen
Laboratory of Natural Product Pesticide, College of Plant Protection, Southwest University, Chongqing 400715, China. trcmei@126.com.
Yanmei
Y
Yu
Laboratory of Natural Product Pesticide, College of Plant Protection, Southwest University, Chongqing 400715, China. lsl203lst@163.com.
Shili
S
Li
Laboratory of Natural Product Pesticide, College of Plant Protection, Southwest University, Chongqing 400715, China. dingw@swu.edu.cn.
Wei
W
Ding
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D018441:Biofilms / Q000187:drug effects / Q000254:growth & development*; D003374:Coumarins / Q000494:pharmacology*; D010935:Plant Diseases / Q000382:microbiology; D043368:Ralstonia solanacearum / Q000502:physiology*; D000077185:Resveratrol; D013267:Stilbenes / Q000494:pharmacology*; D014026:Nicotiana / Q000382:microbiology
D000900:Anti-Bacterial Agents; D003374:Coumarins; D013267:Stilbenes; C030123:coumarin; D000077185:Resveratrol
Coumarin; R. solanacearum; antibacterial activity; bacterial wilt; resveratrol