H2O2and Ca2+-based signaling and associated ion accumulation, antioxidant systems and secondary metabolism orchestrate the response to NaCl stress in perennial ryegrass.
Little is known about the interplay between Ca2+and H2O2signaling in stressed cool-season turfgrass. To understand better how Ca2+and H2O2signals are integrated to enhance grass acclimation to stress conditions, we analyzed the rearrangements of endogenous ion accumulation, antioxidant systems and secondary metabolism in roots, stems and leaves of perennial ryegrass (Lolium perenne L.) treated with exogenous Ca2+and H2O2under salinity. Ca2+signaling remarkably enhanced the physiological response to salt conditions. Ca2+signaling could maintain ROS homeostasis in stressed grass by increasing the responses of antioxidant genes, proteins and enzymes. H2O2signaling could activate ROS homeostasis by inducing antioxidant genes but weakened Ca2+signaling in leaves. Furthermore, the metabolic profiles revealed that sugars and sugar alcohol accounted for 49.5-88.2% of all metabolites accumulation in all treated leaves and roots. However, the accumulation of these sugars and sugar alcohols displayed opposing trends between Ca2+and H2O2application in salt-stressed plants, which suggests that these metabolites are the common regulatory factor for Ca2+and H2O2signals. These findings assist in understanding better the integrated network in Ca2+and H2O2of cool-season turfgrass' response to salinity.
Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, Chinese Academy of Science, Wuhan 430074, Hubei, P.R. China.|Sino-Africa Joint Research Center, Chinese Academy of Science, Wuhan 430074, Hubei, P.R. China.
Tao
T
Hu
Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, Chinese Academy of Science, Wuhan 430074, Hubei, P.R. China.|Sino-Africa Joint Research Center, Chinese Academy of Science, Wuhan 430074, Hubei, P.R. China.
Ke
K
Chen
Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, Chinese Academy of Science, Wuhan 430074, Hubei, P.R. China.|Sino-Africa Joint Research Center, Chinese Academy of Science, Wuhan 430074, Hubei, P.R. China.
Longxing
L
Hu
Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, Chinese Academy of Science, Wuhan 430074, Hubei, P.R. China.|Sino-Africa Joint Research Center, Chinese Academy of Science, Wuhan 430074, Hubei, P.R. China.
Erick
E
Amombo
Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, Chinese Academy of Science, Wuhan 430074, Hubei, P.R. China.|Sino-Africa Joint Research Center, Chinese Academy of Science, Wuhan 430074, Hubei, P.R. China.
Jinmin
J
Fu
D000975:Antioxidants / Q000378:metabolism*; D002118:Calcium / Q000378:metabolism*; D018506:Gene Expression Regulation, Plant; D006861:Hydrogen Peroxide / Q000378:metabolism*; D008129:Lolium / Q000254:growth & development* / Q000378:metabolism; D018515:Plant Leaves / Q000378:metabolism; D010940:Plant Proteins / Q000378:metabolism*; D018517:Plant Roots / Q000378:metabolism; D018547:Plant Stems / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism; D054712:Salinity; D064210:Secondary Metabolism; D013312:Stress, Physiological
D000975:Antioxidants; D010940:Plant Proteins; D017382:Reactive Oxygen Species; D006861:Hydrogen Peroxide; D002118:Calcium