Influences of IL-1b-3953 C>T and MMP-9-1562C >T Gene Variants on Myocardial Infarction Susceptibility in a Subset of the Iranian Population.
OBJECTIVE
IL-1b-3953 C>T and MMP-9-1562C>T variants have been shown to be linked to the development of myocardial infarction (MI), although previous studies have reported inconsistent results. The aim of the present study was to determine whether these genetic variations are associated with MI susceptibility in an Iranian population.


METHODS
In the current study, 117 patients with MI and 120 control group members were selected as participants. Peripheral blood samples were taken from all the subjects for genomic DNA extraction. Single nucleotide polymorphism (SNP) genotyping was performed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) assays.


RESULTS
Multiple logistic regression analysis revealed that the TT genotype of the IL-1b-3953 C>T polymorphism is associated with a significant MI protective effect in: the homozygote model after adjustment for MI risk factors (odds ratio [OR]: 0.18, confidence interval [95% CI] = 0.04-0.72; p = 0.01); and also in the recessive genetic model both before (OR: 0.39, 95% CI: 0.15-0.96, p = 0.04) and after (OR: 0.15, 95% CI: 0.04-0.58, p = 0.006) adjustment for MI risk factors. Furthermore, multiple logistic regression analysis indicated that the individuals with the TT genotype of the MMP-9-1562C>T polymorphism were significantly protected against MI in comparison with the CC genotype (OR: 0.01, 95% CI: 0.002-0.68, p = 0.03).


CONCLUSIONS
The findings suggest that the minor alleles of the two polymorphisms under study both have protective effects with respect to MI susceptibility in the Iranian population.
1 Genetic Department, Faculty of Medicine, Babol University of Medical Sciences, Babol, Iran .
Abdolreza
A
Daraei
2 Noncommunicable Diseases Research Center, Fasa University of Medical Sciences , Fasa, Iran .|3 Department of Medical Genetics, School of Medicine, Tehran University of Medical Sciences , Tehran, Iran .
Yaser
Y
Mansoori
3 Department of Medical Genetics, School of Medicine, Tehran University of Medical Sciences , Tehran, Iran .
Zahra
Z
Zendebad
3 Department of Medical Genetics, School of Medicine, Tehran University of Medical Sciences , Tehran, Iran .
Javad
J
Tavakkoly-Bazzaz
4 Department of Epidemiology and Biostatistics, School of Public Health, Tehran University of Medical Sciences , Tehran, Iran .
Farzan
F
Madadizadeh
2 Noncommunicable Diseases Research Center, Fasa University of Medical Sciences , Fasa, Iran .
Mohammad Mehdi
MM
Naghizadeh
5 Department of Biochemistry, School of Medicine, Fasa University of Medical Sciences , Fasa, Iran .
Ali
A
Arghavani
2 Noncommunicable Diseases Research Center, Fasa University of Medical Sciences , Fasa, Iran .
Behnam
B
Mansoori
D000368:Aged; D000483:Alleles; D016022:Case-Control Studies; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D006801:Humans; D053583:Interleukin-1beta / Q000097:blood / Q000235:genetics* / Q000378:metabolism; D007492:Iran; D008297:Male; D020780:Matrix Metalloproteinase 9 / Q000235:genetics* / Q000378:metabolism; D008875:Middle Aged; D009203:Myocardial Infarction / Q000235:genetics* / Q000378:metabolism; D020641:Polymorphism, Single Nucleotide; D011401:Promoter Regions, Genetic
D053583:Interleukin-1beta; C579270:MMP9 protein, human; D020780:Matrix Metalloproteinase 9
IL-1b-3953 C>T and MMP-9-1562C>T gene variants; genetic association; myocardial infarction susceptibility