Microstructure and antioxidative capacity of the microalgae mutant Chlorella PY-ZU1 during tilmicosin removal from wastewater under 15% CO2.
The response mechanisms of microalgal mutant Chlorella PY-ZU1 cells were investigated in their removal of antibiotic tilmicosin from wastewater under 15% CO2. Low concentrations (0.01-2mgL-1) of tilmicosin in wastewater stimulated the growth of microalgal cells, whereas high concentrations (5-50mgL-1) of tilmicosin significantly inhibited cell growth. When initial tilmicosin concentration increased from 0 to 50mgL-1, fractal dimension of microalgal cells monotonically increased from 1.36 to 1.62 and cell size monotonically decreased from 4.86 to 3.75μm. In parallel, malondialdehyde content, which represented the degree of cellular oxidative damage, monotonically increased from 1.92×10-7to 7.07×10-7nmol cell-1. Superoxide dismutase activity that represented cellular antioxidant capacity first increased from 2.59×10-4to the peak of 6.60×10-4U cell-1, then gradually decreased to 2.39×10-4U cell-1. The maximum tilmicosin removal efficiency of 99.8% by Chlorella PY-ZU1 was obtained at the initial tilmicosin concentration of 50mgL-1.
State Key Laboratory of Clean Energy Utilization, Zhejiang University, Hangzhou 310027, China. Electronic address: juncheng@zju.edu.cn.
Jun
J
Cheng
State Key Laboratory of Clean Energy Utilization, Zhejiang University, Hangzhou 310027, China.
Qing
Q
Ye
State Key Laboratory of Clean Energy Utilization, Zhejiang University, Hangzhou 310027, China.
Zongbo
Z
Yang
State Key Laboratory of Clean Energy Utilization, Zhejiang University, Hangzhou 310027, China.
Weijuan
W
Yang
State Key Laboratory of Clean Energy Utilization, Zhejiang University, Hangzhou 310027, China.
Junhu
J
Zhou
State Key Laboratory of Clean Energy Utilization, Zhejiang University, Hangzhou 310027, China.
Kefa
K
Cen
D000900:Anti-Bacterial Agents / Q000378:metabolism*; D001673:Biodegradation, Environmental; D002245:Carbon Dioxide / Q000494:pharmacology; D002708:Chlorella / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D008315:Malondialdehyde / Q000378:metabolism; D058086:Microalgae / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D009154:Mutation; D010940:Plant Proteins / Q000378:metabolism; D013482:Superoxide Dismutase / Q000378:metabolism; D015645:Tylosin / Q000031:analogs & derivatives* / Q000378:metabolism; D014865:Waste Disposal, Fluid / Q000379:methods; D062065:Wastewater; D014874:Water Pollutants, Chemical / Q000378:metabolism*
D000900:Anti-Bacterial Agents; D002245:Carbon Dioxide; D008315:Malondialdehyde; D010940:Plant Proteins; D013482:Superoxide Dismutase; D015645:Tylosin; D062065:Wastewater; D014874:Water Pollutants, Chemical; C052319:tilmicosin
Antibiotic removal; Antioxidative capacity; Microalgae; Microstructures; Tilmicosin