Assessment of Basic Biological Functions Exerted by miRNAs.
Assessment of cell viability and proliferation under different miRNA expression levels is an important step in the evaluation of basic miRNA functional effects within the cell. Here, we describe the overexpression of miRNA in question in cells achieved by transfection with subsequent examination of cell viability and proliferation over a period of time using the acid phosphatase assay.
Molecular and Cellular Therapeutics, Royal College of Surgeons in Ireland, Dublin, Ireland.|National Children's Research Centre Our Lady's Children's Hospital, Crumlin, Dublin, Ireland.
John
J
Nolan
Molecular and Cellular Therapeutics, Royal College of Surgeons in Ireland, Dublin, Ireland.|National Children's Research Centre Our Lady's Children's Hospital, Crumlin, Dublin, Ireland.
Raymond L
RL
Stallings
Molecular and Cellular Therapeutics, Royal College of Surgeons in Ireland, Dublin, Ireland. olgapiskareva@rcsi.ie.|National Children's Research Centre Our Lady's Children's Hospital, Crumlin, Dublin, Ireland. olgapiskareva@rcsi.ie.
Olga
O
Piskareva
D045744:Cell Line, Tumor; D002470:Cell Survival; D015870:Gene Expression; D020869:Gene Expression Profiling; D006801:Humans; D035683:MicroRNAs / Q000502:physiology*; D034622:RNA Interference; D014162:Transfection
D035683:MicroRNAs
Cell viability; Proliferation; Transfection; miRNA