Effect of Heme Oxygenase-1 on Mitofusin-1 protein in LPS-induced ALI/ARDS in rats.
Acute lung injury (ALI)/acute respiratory distress syndrome (ARDS) is a common and important oxidative stress in the lung. Mitochondrial fusion responds to the normal morphology and function of cells and is finely regulated by mitochondrial fusion proteins, such as mitofusin-1 protein (Mfn1), mitofusin-2 protein (Mfn2) and optical atrophy 1 (OPA1). Additionally, Mfn1 has been identified as the most important protein in mitochondrial fusion. Heme oxygenase-1 (HO-1) is a stress-inducible protein that plays a critical role in protecting against oxidative stress. However, whether the protection of HO-1 is related to mitochondrial fusion is still a question. Thus, our in vitro and in vivo experiments aimed to identify the relationship between HO-1 and Mfn1. Here, we used Hemin and ZnPP-IX as treatments in an in vivo experiment. Then, HO-1 and Mfn1 were measured using RT-PCR and Western blotting. Supernatants were analyzed for MDA, SOD, and ROS. Our results implied that HO-1 upregulation suppressed oxidative stress induced by LPS, and the possible mechanism could be associated with Mfn1 and the PI3K/Akt pathway.
Department of Anesthesiology, Tianjin Nan Kai Hospital, Tianjin Medical University, Tianjin, 300100, China.
Jianbo
J
Yu
Department of Anesthesiology, Tianjin Nan Kai Hospital, Tianjin Medical University, Tianjin, 300100, China.
Ying
Y
Wang
Department of Anesthesiology, Tianjin Nan Kai Hospital, Tianjin Medical University, Tianjin, 300100, China.
Zhen
Z
Li
Department of Anesthesiology, Tianjin Nan Kai Hospital, Tianjin Medical University, Tianjin, 300100, China.
Shuan
S
Dong
Department of Anesthesiology, Tianjin Nan Kai Hospital, Tianjin Medical University, Tianjin, 300100, China.
Dan
D
Wang
Department of Anesthesiology, Tianjin Nan Kai Hospital, Tianjin Medical University, Tianjin, 300100, China.
Lirong
L
Gong
Department of Anesthesiology, Tianjin Nan Kai Hospital, Tianjin Medical University, Tianjin, 300100, China.
Jia
J
Shi
Department of Anesthesiology, Tianjin Nan Kai Hospital, Tianjin Medical University, Tianjin, 300100, China.
Yuan
Y
Zhang
Institute of Acute Abdominal Diseases of Integrated Traditional Chinese and Western Medicine, Tianjin, 300100, China.
Daquan
D
Liu
Department of Anesthesiology, Tianjin Nan Kai Hospital, Tianjin Medical University, Tianjin, 300100, China.
Rui
R
Mu
D055371:Acute Lung Injury / Q000139:chemically induced / Q000378:metabolism* / Q000473:pathology; D000818:Animals; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D006419:Heme Oxygenase (Decyclizing) / Q000096:biosynthesis*; D006427:Hemin / Q000494:pharmacology; D008070:Lipopolysaccharides / Q000633:toxicity*; D008297:Male; D008565:Membrane Proteins / Q000378:metabolism*; D024101:Mitochondrial Proteins / Q000378:metabolism*; D018384:Oxidative Stress / Q000187:drug effects; D011524:Protoporphyrins / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D012128:Respiratory Distress Syndrome / Q000139:chemically induced / Q000378:metabolism* / Q000473:pathology; D015398:Signal Transduction; D015854:Up-Regulation / Q000187:drug effects
D008070:Lipopolysaccharides; D008565:Membrane Proteins; C489709:Mfn1 protein, rat; D024101:Mitochondrial Proteins; D011524:Protoporphyrins; C017803:zinc protoporphyrin; D006427:Hemin; D006419:Heme Oxygenase (Decyclizing); C491523:Hmox1 protein, rat