Methods of Synchronization of Yeast Cells for the Analysis of Cell Cycle Progression.
Cell division is a fascinating and fundamental process that sustains life. By this process, unicellular organisms reproduce and multicellular organisms sustain development, growth, and tissue repair. Division of a mother cell gives rise to two daughter cells according to an ordered set of events within four successive phases called G1 (gap1), S (DNA Synthesis), G2 (gap2), and M (Mitosis) phase. How these different phases are orchestrated to ensure the physical separation of the two daughter cells is a tightly regulated process. Indeed, inappropriate cell division could lead to uncontrolled cell proliferation and ultimately to cancer. Saccharomyces cerevisiae is an excellent model system for unraveling the secrets of cell division. A large community of researchers has chosen budding yeast as a model because of its advantages: rapid growth in simple and economical media, tractable genetics, powerful biochemistry, cell biology, and proteomics approaches. Furthermore, the cell cycle mechanisms, as elucidated in yeast, are conserved in higher eukaryotes. The ability to synchronize and get large numbers of cells in a particular stage of the cell cycle is crucial to properly explore the mechanisms of the cell cycle. An overview of the most common yeast synchronization techniques has been compiled in this chapter.
Rosenstiel Basic Medical Sciences Research Center, Brandeis University, 415 South Street, Waltham, MA, 02454, USA. juanes@brandeis.edu.
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D064199:Cdc20 Proteins / Q000235:genetics; D002452:Cell Count; D018929:Cell Culture Techniques / Q000379:methods*; D002453:Cell Cycle / Q000187:drug effects; D018797:Cell Cycle Proteins / Q000235:genetics; D004261:DNA Replication / Q000187:drug effects; D004271:DNA, Fungal / Q000235:genetics; D006918:Hydroxyurea / Q000494:pharmacology; D000072235:Mating Factor / Q000494:pharmacology; D009154:Mutation; D015739:Nocodazole / Q000494:pharmacology; D012441:Saccharomyces cerevisiae / Q000166:cytology* / Q000187:drug effects / Q000235:genetics; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics; D050257:Tubulin Modulators / Q000494:pharmacology
C577495:CDC20 protein, S cerevisiae; D064199:Cdc20 Proteins; D018797:Cell Cycle Proteins; D004271:DNA, Fungal; D029701:Saccharomyces cerevisiae Proteins; D050257:Tubulin Modulators; C000606786:alpha-factor (Saccharomyces cerevisiae); D000072235:Mating Factor; D015739:Nocodazole; D006918:Hydroxyurea
Alpha-factor; Block-and-release; Elutriation; Hydroxyurea; Nocodazole; Synchronization; Yeast; cdc mutants