Interplay between RNA interference and heat shock response systems in Drosophila melanogaster.
The genome expression pattern is strongly modified during the heat shock response (HSR) to form an adaptive state. This may be partly achieved by modulating microRNA levels that control the expression of a great number of genes that are embedded within the gene circuitry. Here, we investigated the cross-talk between two highly conserved and universal house-keeping systems, the HSR and microRNA machinery, in Drosophila melanogaster We demonstrated that pronounced interstrain differences in the microRNA levels are alleviated after heat shock (HS) to form a uniform microRNA pattern. However, individual strains exhibit different patterns of microRNA expression during the course of recovery. Importantly, HS-regulated microRNAs may target functionally similar HS-responsive genes involved in the HSR. Despite the observed general downregulation of primary microRNA precursor expression as well as core microRNA pathway genes after HS, the levels of many mature microRNAs are upregulated. This indicates that the regulation of miRNA expression after HS occurs at transcriptional and post-transcriptional levels. It was also shown that deletion of all hsp70 genes had no significant effect on microRNA biogenesis but might influence the dynamics of microRNA expression during the HSR.
Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow 119991, Russian Federation.
S Yu
SY
Funikov
Institute of Molecular Genetics, Russian Academy of Sciences, Moscow 123182, Russian Federation.
S S
0000-0002-3171-2997
SS
Ryazansky
University of Oxford, Oxford, UK.
A A
AA
Kanapin
Lomonosov Moscow State University, Moscow 119991, Russian Federation.
M D
MD
Logacheva
Lomonosov Moscow State University, Moscow 119991, Russian Federation.|Institute for Information Transmission Problems of the Russian Academy of Sciences, Moscow 127051, Russian Federation.
A A
AA
Penin
Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow 119991, Russian Federation.
A V
AV
Snezhkina
Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow 119991, Russian Federation.
V Yu
VY
Shilova
Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow 119991, Russian Federation.
D G
DG
Garbuz
Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow 119991, Russian Federation misha672011@yahoo.com.
M B
0000-0001-8554-2121
MB
Evgen'ev
Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow 119991, Russian Federation.
O G
OG
Zatsepina
D000818:Animals; D029721:Drosophila Proteins; D004331:Drosophila melanogaster / Q000145:classification / Q000235:genetics*; D017353:Gene Deletion; D005786:Gene Expression Regulation; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics; D018869:Heat-Shock Response; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods*; D035683:MicroRNAs / Q000235:genetics*; D034622:RNA Interference; D017423:Sequence Analysis, RNA / Q000379:methods*
D029721:Drosophila Proteins; D018840:HSP70 Heat-Shock Proteins; D035683:MicroRNAs
Drosophila melanogaster; heat shock; hsp70; microRNA