Digitization of Endocytic pH by Hybrid Ultra-pH-Sensitive Nanoprobes at Single-Organelle Resolution.
A multispectral hybrid nanotransistor consisting of modular fluorescent block copolymers with discrete and sharp pH transitions in one nanoparticluate system is presented. This nanotransistor probe allows digitized reporting of dynamic maturation at single-organelle resolution over time. This nanotechnology platform offers a powerful tool in fundamental studies of organelle biology, cell signaling in diseases characterized by pH dysregulation in the endosomes or lysosomes.
Department of Pharmacology, Simmons Comprehensive Cancer Center, UT Southwestern Medical Center at Dallas, 5323 Harry Hines Blvd, Dallas, TX, 75390, USA.|Beijing Key Laboratory of Molecular Pharmaceutics and State Key Laboratory of Natural and Biomimetic Drugs, Peking University, Beijing, 100191, China.
Yiguang
Y
Wang
Department of Pharmacology, Simmons Comprehensive Cancer Center, UT Southwestern Medical Center at Dallas, 5323 Harry Hines Blvd, Dallas, TX, 75390, USA.|Department of Cell Biology, UT Southwestern Medical Center at Dallas, 5323 Harry Hines Blvd, Dallas, TX, 75390, USA.
Chensu
C
Wang
Department of Pharmacology, Simmons Comprehensive Cancer Center, UT Southwestern Medical Center at Dallas, 5323 Harry Hines Blvd, Dallas, TX, 75390, USA.
Yang
Y
Li
Department of Pharmacology, Simmons Comprehensive Cancer Center, UT Southwestern Medical Center at Dallas, 5323 Harry Hines Blvd, Dallas, TX, 75390, USA.
Gang
G
Huang
Department of Pharmacology, Simmons Comprehensive Cancer Center, UT Southwestern Medical Center at Dallas, 5323 Harry Hines Blvd, Dallas, TX, 75390, USA.
Tian
T
Zhao
Department of Pharmacology, Simmons Comprehensive Cancer Center, UT Southwestern Medical Center at Dallas, 5323 Harry Hines Blvd, Dallas, TX, 75390, USA.
Xinpeng
X
Ma
Department of Pharmacology, Simmons Comprehensive Cancer Center, UT Southwestern Medical Center at Dallas, 5323 Harry Hines Blvd, Dallas, TX, 75390, USA.
Zhaohui
Z
Wang
Department of Otolaryngology, UT Southwestern Medical Center at Dallas, 5323 Harry Hines Blvd, Dallas, TX, 75390, USA.
Baran D
BD
Sumer
Department of Cell Biology, UT Southwestern Medical Center at Dallas, 5323 Harry Hines Blvd, Dallas, TX, 75390, USA.
Michael A
MA
White
Department of Pharmacology, Simmons Comprehensive Cancer Center, UT Southwestern Medical Center at Dallas, 5323 Harry Hines Blvd, Dallas, TX, 75390, USA.
Jinming
J
Gao
acidification; endocytosis; hybrid nanoprobes; organelle maturation; pH-sensitive nanoparticles