Ocular toxoplasmosis: susceptibility in respect to the genes encoding the KIR receptors and their HLA class I ligands.
The objective of this study was to investigate the influence of the genes encoding the KIR receptors and their HLA ligands in the susceptibility of ocular toxoplasmosis. A total of 297 patients serologically-diagnosed with toxoplasmosis were selected and stratified according to the presence (n = 148) or absence (n = 149) of ocular scars/lesions due to toxoplasmosis. The group of patients with scars/lesions was further subdivided into two groups according to the type of ocular manifestation observed: primary (n = 120) or recurrent (n = 28). Genotyping was performed by PCR-SSOP. Statistical analyses were conducted using the Chi-square test, and odds ratio with a 95% confidence interval was also calculated to evaluate the risk association. The activating KIR3DS1 gene was associated with increased susceptibility for ocular toxoplasmosis. The activating KIR together with their HLA ligands (KIR3DS1-Bw4-80Ile and KIR2DS1+/C2++KIR3DS1+/Bw4-80Ile+) were associated with increased susceptibility for ocular toxoplasmosis and its clinical manifestations. KIR-HLA inhibitory pairs -KIR2DL3/2DL3-C1/C1 and KIR2DL3/2DL3-C1- were associated with decreased susceptibility for ocular toxoplasmosis and its clinical forms, while the KIR3DS1-/KIR3DL1+/Bw4-80Ile+combination was associated as a protective factor against the development of ocular toxoplasmosis and, in particular, against recurrent manifestations. Our data demonstrate that activating and inhibitory KIR genes may influence the development of ocular toxoplasmosis.
Immunogenetics Laboratory, Molecular Biology Department, Faculdade de Medicina de São José do Rio Preto. Avenida Brigadeiro Faria Lima, 5416, Vila São Pedro. CEP: 15090-000 - São José do Rio Preto, SP, Brazil.|FAMERP Toxoplasma Research Group, Faculdade de Medicina de São José do Rio Preto, São José do Rio Preto, SP, Brazil.
Christiane Maria
CM
Ayo
FAMERP Toxoplasma Research Group, Faculdade de Medicina de São José do Rio Preto, São José do Rio Preto, SP, Brazil.|Ophthalmology Outpatient Clinic, Hospital de Base de São José do Rio Preto, Fundação Faculdade Regional de Medicina de São José do Rio Preto. Avenida Brigadeiro Faria Lima, 5544, Vila São José. CEP: 15090-000 - São José do Rio Preto, SP, Brazil.
Fábio Batista
FB
Frederico
Immunogenetics Laboratory, Molecular Biology Department, Faculdade de Medicina de São José do Rio Preto. Avenida Brigadeiro Faria Lima, 5416, Vila São Pedro. CEP: 15090-000 - São José do Rio Preto, SP, Brazil.
Rubens Camargo
RC
Siqueira
Immunogenetics Laboratory, Molecular Biology Department, Faculdade de Medicina de São José do Rio Preto. Avenida Brigadeiro Faria Lima, 5416, Vila São Pedro. CEP: 15090-000 - São José do Rio Preto, SP, Brazil.|FAMERP Toxoplasma Research Group, Faculdade de Medicina de São José do Rio Preto, São José do Rio Preto, SP, Brazil.
Cinara de Cássia
CC
Brandão de Mattos
Immunogenetics Laboratory, Molecular Biology Department, Faculdade de Medicina de São José do Rio Preto. Avenida Brigadeiro Faria Lima, 5416, Vila São Pedro. CEP: 15090-000 - São José do Rio Preto, SP, Brazil.|FAMERP Toxoplasma Research Group, Faculdade de Medicina de São José do Rio Preto, São José do Rio Preto, SP, Brazil.
Mariana
M
Previato
Ophthalmology Outpatient Clinic, Hospital de Base de São José do Rio Preto, Fundação Faculdade Regional de Medicina de São José do Rio Preto. Avenida Brigadeiro Faria Lima, 5544, Vila São José. CEP: 15090-000 - São José do Rio Preto, SP, Brazil.
Amanda Pires
AP
Barbosa
Immunogenetics Laboratory, Molecular Biology Department, Faculdade de Medicina de São José do Rio Preto. Avenida Brigadeiro Faria Lima, 5416, Vila São Pedro. CEP: 15090-000 - São José do Rio Preto, SP, Brazil.|FAMERP Toxoplasma Research Group, Faculdade de Medicina de São José do Rio Preto, São José do Rio Preto, SP, Brazil.
Fernando Henrique Antunes
FH
Murata
Immunogenetics Laboratory, Molecular Biology Department, Faculdade de Medicina de São José do Rio Preto. Avenida Brigadeiro Faria Lima, 5416, Vila São Pedro. CEP: 15090-000 - São José do Rio Preto, SP, Brazil.
Aparecida Perpétuo
AP
Silveira-Carvalho
Immunogenetics Laboratory, Molecular Biology Department, Faculdade de Medicina de São José do Rio Preto. Avenida Brigadeiro Faria Lima, 5416, Vila São Pedro. CEP: 15090-000 - São José do Rio Preto, SP, Brazil.|FAMERP Toxoplasma Research Group, Faculdade de Medicina de São José do Rio Preto, São José do Rio Preto, SP, Brazil.
Luiz Carlos
LC
de Mattos
D000328:Adult; D005260:Female; D020022:Genetic Predisposition to Disease; D006680:HLA Antigens / Q000235:genetics*; D006801:Humans; D008297:Male; D008875:Middle Aged; D054344:Receptors, KIR2DL3 / Q000235:genetics*; D054349:Receptors, KIR3DS1 / Q000235:genetics*; D014126:Toxoplasmosis, Ocular / Q000235:genetics*
D006680:HLA Antigens; C515078:KIR2DL3 protein, human; D054344:Receptors, KIR2DL3; D054349:Receptors, KIR3DS1