Comparison of Antibody Immune responses to different HIV-1 Envelope Glycoprotein Mutants.
The identification of immunogens is crucial for human immunodeficiency virus type 1 (HIV-1) vaccine development. In our previous study, we demonstrated that HIV-1 envelope glycoprotein mutants based on the equine infectious anemia virus (EIAV)attenuated vaccine enhance immunogenicity, both for DNA immunization alone and as a combined DNA prime-vaccinia boost immunization. An RV144 clinical trial has demonstrated that an envelope protein boost may provide some degree of protection against HIV-1 infection. In order to explore the antibody immune responses to two HIV-1 envelope glycoprotein mutants based on the EIAV vaccine and wild-type envelope glycoprotein, mice and guinea pigs were immunized using a DNA prime-protein boost immunization strategy. The result showed, compared with wild-type gp140, gp140 2M (which contained 2 sites amino acid mutations) and gp140 5M (which contained 5 sites amino acid mutations) increased env-specific IgG and IgG3 binding antibody titers.Gp140 2M resulted in a slight improvement in the neutralizing antibody response against sensitive HIV-1 isolates compared with gp140. These findings have implications for HIV-1 vaccine development based on the HIV-1 CN54 envelope glycoprotein.
Jian-Dong
JD
Liu
Li
L
Ren
Bin
B
Ju
Wei
W
Song
Xiang-Yang
XY
Ge
Kun-Xue
KX
Hong
Ying
Y
Liu
Wei
W
Xu
Yan-Ling
YL
Hao
State Key Laboratory for Infectious Disease Prevention and Control National Center for AIDS/STD Control and Prevention, Chinese Center for Disease Control and Prevention 155 Changbai Road, Changping District, Beijing 102206, China. yshao08@gmail.com.
Yi-Ming
YM
Shao