Evaluation of the miRNA profiling and effectiveness of the propolis on B-cell acute lymphoblastic leukemia cell line.
Acute lymphoblastic leukemia (ALL) is one of the most frequent causes of death from cancer. Since the discovery of chemotherapeutic agents, ALL has become a model for improvement of survival. In parallel to this, serious side effects were observed and new natural therapeutic options has been discussed. One of these substances is called propolis which is a resinous substance gathered by honeybees. In the molecular era, miRNAs have been shown to play crucial roles in the development of many clinical conditions. The aim of this study is to evaluate the effect of Aydın propolis on 81 human miRNA activity in CCRF-SB leukemia cell line. Apoptotic effects of propolis on cell lines were also evaluated and apoptosis were found to be induced 1.5 fold in B-cell leukemia cells. The expression of 63 miRNAs (46 miRNAs were downregulated, 19 miRNAs were upregulated) in propolis treated leukemia cells have changed significantly (p<0.05). In conclusion propolis has changed expression of miRNAs which have epigenetic effects on leukemic cells. It is thought that it can be a promising agent for ALL treatment for future studies.
Ege University, Faculty of Medicine, Izmir, Turkey.
Ugur Cem
UC
Yilmaz
Ege University, Faculty of Medicine, Department of Medical Biology, Izmir, Turkey. Electronic address: bakiye.goker@ege.edu.tr.
Bakiye Goker
BG
Bagca
Ege University, Faculty of Medicine, Department of Medical Genetics, Izmir, Turkey.
Emin
E
Karaca
Ege University, Faculty of Medicine, Department of Medical Genetics, Izmir, Turkey.
Asude
A
Durmaz
Ege University, Faculty of Medicine, Department of Medical Genetics, Izmir, Turkey.
Burak
B
Durmaz
Ege University, Faculty of Medicine, Department of Medical Genetics, Izmir, Turkey.
Ayca
A
Aykut
Ege University, Faculty of Pharmacy, Izmir, Turkey.
Husniye
H
Kayalar
Ege University, Faculty of Medicine, Department of Medical Biology, Izmir, Turkey.
Cigir Biray
CB
Avci
Ege University, Faculty of Medicine, Department of Medical Biology, Izmir, Turkey.
Sunde Yilmaz
SY
Susluer
Ege University, Faculty of Medicine, Department of Medical Biology, Izmir, Turkey.
Cumhur
C
Gunduz
Ege University, Faculty of Medicine, Department of Medical Genetics, Izmir, Turkey.
Ozgur
O
Cogulu
D017209:Apoptosis / Q000187:drug effects; D063646:Carcinogenesis / Q000235:genetics / Q000473:pathology; D045744:Cell Line, Tumor; D020869:Gene Expression Profiling; D015973:Gene Expression Regulation, Leukemic / Q000187:drug effects*; D006801:Humans; D020128:Inhibitory Concentration 50; D015448:Leukemia, B-Cell / Q000235:genetics*; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000235:genetics*; D011429:Propolis / Q000494:pharmacology*
D035683:MicroRNAs; D011429:Propolis
Acute lymphoblastic leukemia; Apoptosis; Propolis; miRNA