Hierarchically mesostructured porous TiO2hollow nanofibers for high performance glucose biosensing.
Effective immobilization of enzymes on an electrode surface is of great importance for biosensor development, but it still remains challenging because enzymes tend to denaturation and/or form close-packed structures. In this work, a free-standing TiO2hollow nanofibers (HNF-TiO2) was successfully prepared by a simple and scalable electrospun nanofiber film template-assisted sol-gel method, and was further explored for glucose oxidase (GOD) immobilization and biosensing. This porous and nanotubular HNF-TiO2provides a well-defined hierarchical nanostructure for GOD loading, and the fine TiO2nanocrystals facilitate direct electron transfer from GOD to the electrode, also the strong interaction between GOD and HNF-TiO2greatly enhances the stability of the biosensor. The as-prepared glucose biosensors show good sensing performances both in O2-free and O2-containing conditions with good sensitivity, satisfactory selectivity, long-term stability and sound reliability. The novel textile formation, porous and hierarchically mesostructured nature of HNF-TiO2with excellent analytical performances make it a superior platform for the construction of high-performance glucose biosensors.
Department of Chemistry and Chemical Engineering, Jiangxi Normal University, Nanchang, Jiangxi 330022, China. Electronic address: guoqiaohui@jxnu.edu.cn.
Qiaohui
Q
Guo
Department of Chemistry and Chemical Engineering, Jiangxi Normal University, Nanchang, Jiangxi 330022, China.
Lijuan
L
Liu
Department of Chemistry and Chemical Engineering, Jiangxi Normal University, Nanchang, Jiangxi 330022, China.
Man
M
Zhang
Department of Chemistry and Chemical Engineering, Jiangxi Normal University, Nanchang, Jiangxi 330022, China.
Haoqing
H
Hou
Department of Chemistry and Chemical Engineering, Jiangxi Normal University, Nanchang, Jiangxi 330022, China.
Yonghai
Y
Song
Department of Chemistry and Chemical Engineering, Jiangxi Normal University, Nanchang, Jiangxi 330022, China.
Huadong
H
Wang
Department of Chemistry and Chemical Engineering, Jiangxi Normal University, Nanchang, Jiangxi 330022, China.
Baoying
B
Zhong
Department of Chemistry and Chemical Engineering, Jiangxi Normal University, Nanchang, Jiangxi 330022, China.
Li
L
Wang
D015374:Biosensing Techniques / Q000379:methods*; D001786:Blood Glucose / Q000032:analysis*; D004800:Enzymes, Immobilized / Q000737:chemistry; D005949:Glucose Oxidase / Q000737:chemistry; D006801:Humans; D057230:Limit of Detection; D057139:Nanofibers / Q000737:chemistry* / Q000648:ultrastructure; D015203:Reproducibility of Results; D014025:Titanium / Q000737:chemistry*
D001786:Blood Glucose; D004800:Enzymes, Immobilized; C009495:titanium dioxide; D014025:Titanium; D005949:Glucose Oxidase
Direct electron transfer; Glucose biosensor; Glucose oxidase; Sol-gel method; TiO(2) hollow nanofiber