Percutaneous Transluminal Angioplasty in Patients with Peripheral Arterial Disease Does Not Affect Circulating Monocyte Subpopulations.
Monocytes are mononuclear cells characterized by distinct morphology and expression of CD14 and CD16 surface receptors. Classical, quiescent monocytes are positive for CD14 (lipopolysaccharide receptor) but do not express Fc gamma receptor III (CD16). Intermediate monocytes coexpress CD16 and CD14. Nonclassical monocytes with low expression of CD14 represent mature macrophage-like monocytes. Monocyte behavior in peripheral arterial disease (PAD) and during vessel wall directed treatment is not well defined. This observation study aimed at monitoring of acute changes in monocyte subpopulations during percutaneous transluminal angioplasty (PTA) in PAD patients. Patients with Rutherford 3 and 4 PAD with no signs of inflammatory process underwent PTA of iliac, femoral, or popliteal segments. Flow cytometry for CD14, CD16, HLA-DR, CD11b, CD11c, and CD45RA antigens allowed characterization of monocyte subpopulations in blood sampled before and after PTA (direct angioplasty catheter sampling). Patients were clinically followed up for 12 months. All 61 enrolled patients completed 12-month follow-up. Target vessel failure occurred in 12 patients. While absolute counts of monocyte were significantly lower after PTA, only subtle monocyte activation after PTA (CD45RA andβ-integrins) occurred. None of the monocyte parameters correlated with long-term adverse clinical outcome. Changes in absolute monocyte counts and subtle changes towards an activation phenotype after PTA may reflect local cell adhesion phenomenon in patients with Rutherford 3 or 4 peripheral arterial disease.
Department of Angiology, Jagiellonian University Medical College, Kraków, Poland; ANGIO-MEDICUS Angiology Clinic, Kraków, Poland.
Pawel
0000-0002-0944-0972
P
Maga
Department of Internal and Agricultural Medicine, Jagiellonian University Medical College, Kraków, Poland; Institute of Cardiovascular and Medical Sciences, University of Glasgow, Glasgow, UK.
Tomasz P
0000-0002-7990-2896
TP
Mikolajczyk
Department of Angiology, Jagiellonian University Medical College, Kraków, Poland; ANGIO-MEDICUS Angiology Clinic, Kraków, Poland.
Lukasz
0000-0002-8853-639X
L
Partyka
ANGIO-MEDICUS Angiology Clinic, Kraków, Poland.
Marek
M
Krzanowski
Faculty of Health Sciences, Jagiellonian University Medical College, Kraków, Poland.
Krzysztof P
KP
Malinowski
Department of Angiology, Jagiellonian University Medical College, Kraków, Poland.
Rafal
R
Nizankowski
D017130:Angioplasty / Q000379:methods*; D015703:Antigens, CD / Q000378:metabolism; D015415:Biomarkers / Q000378:metabolism; D003710:Demography; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D008297:Male; D008875:Middle Aged; D009000:Monocytes / Q000473:pathology*; D058729:Peripheral Arterial Disease / Q000473:pathology*
D015703:Antigens, CD; D015415:Biomarkers