Trypanosoma cruzi infection in captive Neotropical primates in the Brazilian Amazon.
The aim of this study was to detect the infection by Trypanosoma cruzi in captive Neotropical primates in the Brazilian Amazon. From February 2013 to July 2014, 112 blood samples were collected from Neotropical primates from the Amazonas, Amapá, and Pará States, north of Brazil. The subjects belonged to the families Cebidae (N = 59), Atelidae (N = 41), Callitrichidae (N = 5), Pitheciidae (N = 4), and Aotidae (N = 3). Blood smears also were examined for the presence of trypomastigotes by optical microscopy. For the detection of T. cruzi DNA, a Nested-PCR with primers TCZ1/TCZ2 and TCZ3/TCZ4 was performed. T. cruzi DNA was detected in 12.5% (14/112) of Neotropical primates examined. Positive samples were detected in 16%, 12.5%, and 11.11% of the different species of primates sampled from the Amapá, Pará, and Amazonas states, respectively. The analysis of the blood smears did not reveal trypomastigote forms of T. cruzi. In conclusion, Neotropical primates kept in captivity were infected by T. cruzi in the studied areas. We recommend that a health management protocol be put into place to prevent the transmission of infectious agents among captive populations, captive and wild populations, and between NHPs and the technicians who handle these animals.
Universidade Federal do Pará (UFPA), Instituto de Medicina Veterinária, Laboratório de Parasitologia Animal, Castanhal, Pará, Brazil.
Michele
M
Bahia
Universidade Federal do Pará (UFPA), Instituto de Medicina Veterinária, Laboratório de Parasitologia Animal, Castanhal, Pará, Brazil.
Flávia
F
de Nazaré Leite Barros
Universidade Federal do Pará (UFPA), Instituto de Medicina Veterinária, Laboratório de Parasitologia Animal, Castanhal, Pará, Brazil.
Paulo Cesar
PC
Magalhães-Matos
Universidade Federal do Pará (UFPA), Instituto de Medicina Veterinária, Laboratório de Parasitologia Animal, Castanhal, Pará, Brazil.
Thamirys
T
de Souza Gonçalves
Centro de Triagem de Animais Silvestres Municipal Refúgio da Vida Silvestre Sauim Castanheiras, Manaus, Amazonas, Brazil.
Laerzio
L
Chiesorin Neto
Instituto Chico Mendes de Biodiversidade (ICMBIO), Manaus, Amazonas, Brazil.
Diogo Cesar Lagroteria
DC
Oliveira Faria
Instituto Brasileiro de Meio Ambiente e dos Recursos Naturais Renováveis (IBAMA), Macapá, Amapá, Brazil.
Sandra
S
Aparecida Romeiro
Instituto Brasileiro de Meio Ambiente e dos Recursos Naturais Renováveis (IBAMA), Macapá, Amapá, Brazil.|Universidade Federal Rural da Amazônia (UFRA), Instituto de Saúde e Produção Animal, Belém, Pará, Brazil.
Frederico Ozanan
FO
Barros Monteiro
Universidade Federal do Pará (UFPA), Instituto de Medicina Veterinária, Laboratório de Parasitologia Animal, Castanhal, Pará, Brazil.
Gustavo
G
Góes-Cavalcante
Universidade Federal do Pará (UFPA), Instituto de Medicina Veterinária, Laboratório de Parasitologia Animal, Castanhal, Pará, Brazil.
Alessandra
A
Scofield
D000818:Animals; D016645:Aotidae; D001938:Brazil; D014355:Chagas Disease / Q000635:transmission / Q000662:veterinary; D011323:Primates / Q000469:parasitology*; D014349:Trypanosoma cruzi / Q000472:pathogenicity*
Trypanosoma cruzi; amazon; primates; trypanosomatidae