Evaluation of Magnetized-Erythrocyte Group Antigens to Detect ABO Antibodies.
Screening for IgM titers of anti-A and anti-B is recommended when providing ABO incompatible platelet transfusion. The life-time of reagent cells depends upon the preservative diluents. We aimed to evaluate the IgM titers of anti-A and anti-B testing with magnetized-erythrocyte group antigens (MEGA) and fresh RBCs and study the relationship of ABO antibody titers between both techniques. Altogether, 100 serum samples from group O donors at the National Blood Centre, Thai Red Cross Society, Bangkok, Thailand were included. EDTA blood from three different A and B blood group individuals was prepared as fresh reagent RBCs and MEGA. Each serum sample was tested simultaneously for IgM anti-A and anti-B titers using fresh RBCs and MEGA by standard tube technique. Antibody titers were compared between both techniques. Test for reproducibility and stability of MEGA were performed. The IgM anti-A and anti-B titers using fresh RBCs yielded higher agglutination scores than MEGA (P< 0.001). However, a good correlation was obtained in the agglutination titers (anti-A,r= 0.838 and anti-B,r= 0.877). The mean and standard deviation of anti-A and anti-B titers using MEGA from five sera in triplicate showed no significant difference (P> 0.05). Moreover, the titer test results using MEGA after dilution remained stable up to 8 h. The MEGA can be used as a replacement for fresh RBCs to perform ABO serum grouping. It is simple to use, avoids centrifugation and provides good results in terms of stability and reproducibility.
Graduate Program, Faculty of Health Sciences, Thammasat University, Pathum Thani, 12120 Thailand.
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Nathalang
Graduate Program, Faculty of Health Sciences, Thammasat University, Pathum Thani, 12120 Thailand.
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Intharanut
Graduate Program, Faculty of Health Sciences, Thammasat University, Pathum Thani, 12120 Thailand.
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Sriwanitchrak
National Blood Centre, Thai Red Cross Society, Bangkok, Thailand.
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Setthakarn
Souzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou, China.
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Duan
Souzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou, China.
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Wang
Souzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou, China.
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Ding
Souzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou, China.
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Li
Anti-A; Anti-B; IgM titers; Magnetized-erythrocyte group antigens