METHYLENE BLUE SENSITIVITY 1 (MBS1) is required for acclimation of Arabidopsis to singlet oxygen and acts downstream of β-cyclocitral.
Singlet oxygen (1O2) signalling in plants is essential to trigger both acclimatory mechanisms and programmed cell death under high light stress. However, because of its chemical features,1O2requires mediators, and the players involved in this pathway are largely unknown. The β-carotene oxidation product, β-cyclocitral, is one such mediator. Produced in the chloroplast, β-cyclocitral induces changes in nuclear gene expression leading to photoacclimation. Recently, the METHYLENE BLUE SENSITIVITY protein MBS has been identified as a key player in1O2signalling leading to tolerance to high light. Here, we provide evidence that MBS1 is essential for acclimation to1O2and cross-talks with β-cyclocitral to mediate transfer of the1O2signal to the nucleus, leading to photoacclimation. The presented results position MBS1 downstream of β-cyclocitral in1O2signalling and suggest an additional role for MBS1 in the regulation of plant growth and development under chronic1O2production.
CEA Cadarache, CNRS UMR 7265, Aix-Marseille Université, Laboratoire d'Ecophysiologie Moléculaire des Plantes, F-13108, Saint-Paul-lez-Durance, France.
Leonard
L
Shumbe
CEA Cadarache, CNRS UMR 7265, Aix-Marseille Université, Laboratoire d'Ecophysiologie Moléculaire des Plantes, F-13108, Saint-Paul-lez-Durance, France.
Stefano
0000-0002-0464-5549
S
D'Alessandro
State Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, 100101, Beijing, China.|Max-Planck-Institut für Molekulare Pflanzenphysiologie, 14476, Potsdam-Golm, Germany.
Ning
N
Shao
CEA Cadarache, CNRS UMR 7265, Aix-Marseille Université, Laboratoire d'Ecophysiologie Moléculaire des Plantes, F-13108, Saint-Paul-lez-Durance, France.
Anne
A
Chevalier
CEA Cadarache, CNRS UMR 7265, Aix-Marseille Université, Laboratoire d'Ecophysiologie Moléculaire des Plantes, F-13108, Saint-Paul-lez-Durance, France.
Brigitte
B
Ksas
Max-Planck-Institut für Molekulare Pflanzenphysiologie, 14476, Potsdam-Golm, Germany.
Ralph
R
Bock
CEA Cadarache, CNRS UMR 7265, Aix-Marseille Université, Laboratoire d'Ecophysiologie Moléculaire des Plantes, F-13108, Saint-Paul-lez-Durance, France.
Michel
M
Havaux
D000064:Acclimatization / Q000528:radiation effects; D000447:Aldehydes / Q000494:pharmacology*; D017360:Arabidopsis / Q000235:genetics / Q000254:growth & development / Q000502:physiology* / Q000528:radiation effects; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D004224:Diterpenes / Q000494:pharmacology*; D018506:Gene Expression Regulation, Plant / Q000187:drug effects / Q000528:radiation effects; D049452:Green Fluorescent Proteins / Q000378:metabolism; D008027:Light; D009154:Mutation / Q000235:genetics; D010641:Phenotype; D010788:Photosynthesis / Q000528:radiation effects; D018515:Plant Leaves / Q000502:physiology / Q000528:radiation effects; D026082:Singlet Oxygen / Q000494:pharmacology*; D013312:Stress, Physiological / Q000528:radiation effects
D000447:Aldehydes; D029681:Arabidopsis Proteins; D004224:Diterpenes; D049452:Green Fluorescent Proteins; D026082:Singlet Oxygen; C516118:beta-cyclocitral
carotenoid oxidation; high light stress; signalling