Efficient delivery of therapeutic siRNA into glioblastoma cells using multifunctional dendrimer-entrapped gold nanoparticles.
OBJECTIVE
To synthesize the arginine-glycine-aspartic (RGD) functionalized dendrimer-entrapped gold nanoparticles (Au DENPs) for siRNA delivery to induce gene silencing of cancer cells in vitro and in vivo.


METHODS
Au DENPs modified with RGD peptide via a polyethylene glycol spacer were used as a vector of two distinct small interfering RNAs (siRNAs) (VEGFvascular endothelial growth factor siRNA and B-cell lymphoma/leukemia-2 siRNA), and the physicochemical properties, cytocompatibility and transfection efficiency of Au DENP/siRNA polyplexes were characterized.


RESULTS
The Au DENP/siRNA polyplexes with good cytocompatibility and highly efficient transfection capacity can be used for the transfection of siRNAs.


CONCLUSIONS
The developed functional RGD-modified Au DENPs may be used for efficient gene therapy of different types of cancer.
State Key Laboratory for Modification of Chemical Fibers & Polymer Materials, College of Chemistry, Chemical Engineering & Biotechnology, Donghua University, Shanghai 201620, People's Republic of China.
Lingdan
L
Kong
State Key Laboratory for Modification of Chemical Fibers & Polymer Materials, College of Chemistry, Chemical Engineering & Biotechnology, Donghua University, Shanghai 201620, People's Republic of China.
Yilun
Y
Wu
CQM-Centro de Quimica da Madeira, Universidade da Madeira, Campus da Penteada, 9000-390 Funchal, Portugal.
Carla S
CS
Alves
State Key Laboratory for Modification of Chemical Fibers & Polymer Materials, College of Chemistry, Chemical Engineering & Biotechnology, Donghua University, Shanghai 201620, People's Republic of China.|CQM-Centro de Quimica da Madeira, Universidade da Madeira, Campus da Penteada, 9000-390 Funchal, Portugal.
Xiangyang
X
Shi
D000818:Animals; D045744:Cell Line, Tumor; D050091:Dendrimers / Q000737:chemistry*; D016503:Drug Delivery Systems; D065546:Drug Liberation; D020868:Gene Silencing; D005909:Glioblastoma; D006046:Gold / Q000737:chemistry*; D006801:Humans; D053768:Metal Nanoparticles / Q000737:chemistry*; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009842:Oligopeptides / Q000737:chemistry; D010316:Particle Size; D011092:Polyethylene Glycols / Q000737:chemistry; D034741:RNA, Small Interfering / Q000008:administration & dosage* / Q000737:chemistry; D014162:Transfection
D050091:Dendrimers; D009842:Oligopeptides; D034741:RNA, Small Interfering; D011092:Polyethylene Glycols; D006046:Gold; C047981:arginyl-glycyl-aspartic acid
RGD peptide; dendrimer-entrapped gold nanoparticles; gene silencing; siRNA