Phylogenetic and Diversity Analysis of Dactylis glomerata Subspecies Using SSR and IT-ISJ Markers.
The genusDactylis, an important forage crop, has a wide geographical distribution in temperate regions. While this genus is thought to include a single species,Dactylis glomerata, this species encompasses many subspecies whose relationships have not been fully characterized. In this study, the genetic diversity and phylogenetic relationships of nine representativeDactylissubspecies were examined using SSR and IT-ISJ markers. In total, 21 pairs of SSR primers and 15 pairs of IT-ISJ primers were used to amplify 295 polymorphic bands with polymorphic rates of 100%. The average polymorphic information contents (PICs) of SSR and IT-ISJ markers were 0.909 and 0.780, respectively. The combined data of the two markers indicated a high level of genetic diversity among the nineD. glomeratasubspecies, with a Nei's gene diversity index value of 0.283 and Shannon's diversity of 0.448. Preliminarily phylogenetic analysis results revealed that the 20 accessions could be divided into three groups (A, B, C). Furthermore, they could be divided into five clusters, which is similar to the structure analysis with K = 5. Phylogenetic placement in these three groups may be related to the distribution ranges and the climate types of the subspecies in each group. Group A contained eight accessions of four subspecies, originating from the west Mediterranean, while Group B contained seven accessions of three subspecies, originating from the east Mediterranean.
Department of Grassland Science, Animal Science and Technology College, Sichuan Agricultural University, Chengdu 611130, China. yandfoo001l@163.com.
Defei
D
Yan
Department of Grassland Science, Animal Science and Technology College, Sichuan Agricultural University, Chengdu 611130, China. zxx19890727@163.com.
Xinxin
X
Zhao
Department of Grassland Science, Animal Science and Technology College, Sichuan Agricultural University, Chengdu 611130, China. chengyajuan_sau@hotmail.com.
Yajuan
Y
Cheng
Department of Grassland Science, Animal Science and Technology College, Sichuan Agricultural University, Chengdu 611130, China. maroar@126.com.
Xiao
X
Ma
Department of Grassland Science, Animal Science and Technology College, Sichuan Agricultural University, Chengdu 611130, China. huanglinkai@sicau.edu.cn.
Linkai
L
Huang
Department of Grassland Science, Animal Science and Technology College, Sichuan Agricultural University, Chengdu 611130, China. zhangxq@sicau.edu.cn.
Xinquan
X
Zhang
D018744:DNA, Plant / Q000235:genetics*; D031745:Dactylis / Q000235:genetics*; D005819:Genetic Markers; D014644:Genetic Variation; D010802:Phylogeny
D018744:DNA, Plant; D005819:Genetic Markers
Dactylis; IT-ISJ; SSR; genetic diversity; phylogenetic analysis; subspecies