Analysis of E2 gene integrity in HPV16 and HPV58 viruses isolated from women with cervical pathology.
Integration of human papillomavirus (HPV) DNA into human cells accompanied by the disruption of the viral genome has been described as a prerequisite for cancer development. This study aimed to investigate E2 gene integrity of HPV16 and HPV58 viruses isolated from infected women with cervical lesions. Forty-two HPV16- and 31 HPV58-positive samples were analysed. E2 integrity was assumed when all fragments covering the E2 gene were amplified with specific polymerase chain reaction primers. Overall, in 59% of the samples, at least one fragment was not amplified in HPV16- (57%) and HPV58-positive samples (61%). Samples from high-grade squamous intraepithelial lesions had the highest frequency of E2 gene disruptions (73%), followed by samples from low-grade squamous intraepithelial lesions (63%) and, finally, samples from invasive cervical cancer (35%). Association between the integrity status of the E2 gene, and lesion grade was assessed by the chi-squared test applied to the combined set of viruses (p = 0.6555) or to populations of the same virus type (HPV58, p = 0.3101; HPV16, p = 0.3024). In conclusion, in this study, no association was found between the presence of E2 gene disruptions and the grade of cervical lesions caused by HPV16 and HPV58.
Universidad Autónoma de Yucatán, Centro de Investigaciones Regionales Dr Hideyo Noguchi, Laboratorio de Virología, Mérida, Yucatán, México.
María Del R
MD
González-Losa
Universidad Autónoma de Yucatán, Centro de Investigaciones Regionales Dr Hideyo Noguchi, Laboratorio de Virología, Mérida, Yucatán, México.
Marylin
M
Puerto-Solis
Universidad Autónoma de Yucatán, Centro de Investigaciones Regionales Dr Hideyo Noguchi, Laboratorio de Virología, Mérida, Yucatán, México.
Juan
J
Tenorio Ruiz
Clínica de Colposcopía, Hospital General O'Horán, Mérida, Yucatán, México.
Ariel I
AI
Rosado-López
Universidad Autónoma de Yucatán, Centro de Investigaciones Regionales Dr Hideyo Noguchi, Laboratorio de Virología, Mérida, Yucatán, México.
Oscar
O
Hau-Aviles
Universidad Autónoma de Yucatán, Centro de Investigaciones Regionales Dr Hideyo Noguchi, Laboratorio de Virología, Mérida, Yucatán, México.
Guadalupe
G
Ayora-Talavera
Clínica de Colposcopía, Hospital General Valladolid, Valladolid, Yucatán, México.
Isidro
I
Cisneros-Cutz
Universidad Autónoma de Yucatán, Centro de Investigaciones Regionales Dr Hideyo Noguchi, Laboratorio de Virología, Mérida, Yucatán, México.
Laura
L
Conde-Ferráez
D002294:Carcinoma, Squamous Cell / Q000821:virology*; D003430:Cross-Sectional Studies; D004279:DNA, Viral / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics*; D005260:Female; D052162:Human papillomavirus 16 / Q000235:genetics*; D006801:Humans; D009856:Oncogene Proteins, Viral / Q000235:genetics*; D030361:Papillomavirus Infections / Q000150:complications / Q000821:virology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D002583:Uterine Cervical Neoplasms / Q000821:virology*; D002578:Uterine Cervical Dysplasia / Q000821:virology*
D004279:DNA, Viral; D004268:DNA-Binding Proteins; C054855:E2 protein, Human papillomavirus type 16; D009856:Oncogene Proteins, Viral