Fecal microbiota in pouchitis and ulcerative colitis.
OBJECTIVE
To investigate the changes in microbiota in feces of patients with ulcerative colitis (UC) and pouchitis using genomic technology.


METHODS
Fecal samples were obtained from UC patients with or without an ileal pouch-anal anastomosis (IPAA) procedure, as well as healthy controls. The touchdown polymerase chain reaction technique was used to amplify the whole V3 region of the 16S rRNA gene, which was transcribed from DNA extracted from fecal samples. Denaturing gradient gel electrophoresis was used to separate the amplicons. The band profiles and similarity indices were analyzed digitally. The predominant microbiota in different groups was confirmed by sequencing the 16S rRNA gene.


RESULTS
Microbial biodiversity in the healthy controls was significantly higher compared with the UC groups (P< 0.001) and IPAA groups (P< 0.001). Compared with healthy controls, the UC patients in remission and those in the mildly active stage, the predominant species in patients with moderately and severely active UC changed obviously. In addition, the proportion of the dominant microbiota, which was negatively correlated with the disease activity of UC (r= -6.591,P< 0.01), was decreased in pouchitis patients. The numbers of two types of bacteria,Faecalibacterium prausnitziiandEubacterium rectale, were reduced in UC. Patients with pouchitis had an altered microbiota composition compared with UC patients. The microbiota from pouchitis patients was less diverse than that from severely active UC patients. Sequencing results showed that similar microbiota, such asClostridium perfringens, were shared in both UC and pouchitis.


CONCLUSIONS
Less diverse fecal microbiota was present in patients with UC and pouchitis. IncreasedC. perfringensin feces suggest its role in the exacerbation of UC and pouchitis.
Kai-Yu Li, Jiang-Peng Wei, Sen-Yang Gao, Ying-Ying Zhang, Li-Tian Wang, Gang Liu, Department of General Surgery, Tianjin Medical University General Hospital, Tianjin 300052, China.
Kai-Yu
KY
Li
Kai-Yu Li, Jiang-Peng Wei, Sen-Yang Gao, Ying-Ying Zhang, Li-Tian Wang, Gang Liu, Department of General Surgery, Tianjin Medical University General Hospital, Tianjin 300052, China.
Jian-Lin
JL
Wang
Kai-Yu Li, Jiang-Peng Wei, Sen-Yang Gao, Ying-Ying Zhang, Li-Tian Wang, Gang Liu, Department of General Surgery, Tianjin Medical University General Hospital, Tianjin 300052, China.
Jiang-Peng
JP
Wei
Kai-Yu Li, Jiang-Peng Wei, Sen-Yang Gao, Ying-Ying Zhang, Li-Tian Wang, Gang Liu, Department of General Surgery, Tianjin Medical University General Hospital, Tianjin 300052, China.
Sen-Yang
SY
Gao
Kai-Yu Li, Jiang-Peng Wei, Sen-Yang Gao, Ying-Ying Zhang, Li-Tian Wang, Gang Liu, Department of General Surgery, Tianjin Medical University General Hospital, Tianjin 300052, China.
Ying-Ying
YY
Zhang
Kai-Yu Li, Jiang-Peng Wei, Sen-Yang Gao, Ying-Ying Zhang, Li-Tian Wang, Gang Liu, Department of General Surgery, Tianjin Medical University General Hospital, Tianjin 300052, China.
Li-Tian
LT
Wang
Kai-Yu Li, Jiang-Peng Wei, Sen-Yang Gao, Ying-Ying Zhang, Li-Tian Wang, Gang Liu, Department of General Surgery, Tianjin Medical University General Hospital, Tianjin 300052, China.
Gang
G
Liu
D016022:Case-Control Studies; D003093:Colitis, Ulcerative / Q000382:microbiology*; D005243:Feces / Q000382:microbiology; D006801:Humans; D019449:Pouchitis / Q000382:microbiology*
Disease activity index; Ileal pouch-anal anastomosis; Intestinal flora; Pouchitis; Ulcerative colitis