Trichoderma asperellum spore dose depended modulation of plant growth in vegetable crops.
In the present study we bioprimed seeds of six vegetable crops (tomato, brinjal, chilli, okra, ridge gourd and guar) with different spore doses of Trichoderma asperellum BHUT8 ranging from 102to 108spores ml-1and the effect of biopriming was seen on seed germination and development. The most effective spore dose for enhancement in seed germination and radicle length was found to be 103spores ml-1in tomato and ridge gourd, 104spores ml-1in brinjal and okra while 106spores ml-1in chilli and guar. At the most effective spore dose, the increase in germination percentage was 5, 146.15, 112.5, 5.4, 28.13 and 0% while increase in radicle length was 73.17, 50.83, 171.6, 107.35, 247.19 and 90.79% in tomato, brinjal, chilli, okra, ridge gourd and guar, respectively, compared to their controls. Higher spore dose i.e. 107-108spores ml-1and 106-108spores ml-1in tomato and brinjal, respectively, reduced seed germination percentage and radicle growth compared to their controls. Biopriming with T. asperellum BHUT8 also triggered various defense like responses such as high phenylpropanoid activities and lignifications in bioprimed tomato seedlings compared to the non-bioprimed tomato seedlings demonstrating possible use of BHUT8 against phytopathogens.
Department of Botany, Institute of Science, Banaras Hindu University, Varanasi, India; Department of Mycology and Plant Pathology, Institute of Agricultural Sciences, Banaras Hindu University, Varanasi, India.
Vivek
V
Singh
Department of Botany, Institute of Science, Banaras Hindu University, Varanasi, India.
Ram Sanmukh
RS
Upadhyay
Department of Mycology and Plant Pathology, Institute of Agricultural Sciences, Banaras Hindu University, Varanasi, India.
Birinchi Kumar
BK
Sarma
Department of Mycology and Plant Pathology, Institute of Agricultural Sciences, Banaras Hindu University, Varanasi, India. Electronic address: hbs1@rediffmail.com.
Harikesh Bahadur
HB
Singh
D018517:Plant Roots / Q000254:growth & development / Q000382:microbiology; D012639:Seeds / Q000254:growth & development / Q000382:microbiology; D013172:Spores, Fungal / Q000254:growth & development*; D014242:Trichoderma / Q000254:growth & development*; D014675:Vegetables / Q000254:growth & development* / Q000382:microbiology*
Plant growth promotion; Seed germination; Spore dose; Trichoderma asperellum; Vegetable crops