Morphological and Mechanical Properties of Osteosarcoma Microenvironment Cells Explored by Atomic Force Microscopy.
Cell mechanical properties that depend on cytoskeleton architecture are critical to the mechanotransduction process, and have great potential for cancer diagnosis and therapy. In this study, the morphological and mechanical properties of typical osteosarcoma microenvironment cells, including mesenchymal stem cells (MSC), normal human osteoblast cells (NHOst) and osteosarcoma cells (MG-63), were compared using atomic force microscopy (AFM). The MG-63 cells were smaller and thicker than the MSC and NHOst cells. The membrane roughness of MG-63 cells was higher than that of MSC and NHOst cells. The MG-63 cells had lower stiffness than their normal counterparts due to their reduced organization of the cytoskeleton structure. The cell stiffness influenced the mechanotransduction. The MG-63 cells had a lower percentage of nuclear YAP/TAZ compared with the MSC and NHOst cells. The F-actin assembly was disrupted by the cytochalasin D (cyto D) treatment used to investigate its influence on mechanotransduction. Disruption of the cytoskeleton leaded to a decrease of the cell stiffness, and reduced the nuclear YAP/TAZ percentage, indicating its inhibition in the cell mechanotransduction process. This study would shed light on the development of a novel cancer diagnosis strategy and would contribute to reveal the relationship between the cytoskeleton structure and the cell mechanical properties.
International Center for Materials Nanoarchitectonics, National Institute for Materials Science.
Xinlong
X
Wang
Yingjun
Y
Yang
Xiaohong
X
Hu
Naoki
N
Kawazoe
Yingnan
Y
Yang
Guoping
G
Chen
D000199:Actins / Q000737:chemistry; D001859:Bone Neoplasms / Q000473:pathology*; D045744:Cell Line, Tumor; D002478:Cells, Cultured; D015638:Cytochalasin D / Q000737:chemistry; D003599:Cytoskeleton / Q000737:chemistry; D006801:Humans; D040542:Mechanotransduction, Cellular; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D018625:Microscopy, Atomic Force; D010006:Osteoblasts / Q000166:cytology; D012516:Osteosarcoma / Q000473:pathology*; D059016:Tumor Microenvironment
D000199:Actins; D015638:Cytochalasin D