Solution-Phase Exome Capture.
This protocol describes the construction of a paired-end library of genomic DNA (gDNA) and subsequent capture of specific regions of a genome using NimbleGen sequence capture probes and Illumina TruSeq oligos. The captured DNA, purified and quantitated, is appropriate for use as template in Illumina sequencing systems. A procedure is also provided for magnetic AMPure bead cleanup.
Elaine
E
Mardis
W Richard
WR
McCombie
D004247:DNA / Q000235:genetics* / Q000302:isolation & purification*; D059472:Exome; D015723:Gene Library; D008967:Molecular Biology / Q000379:methods*; D017422:Sequence Analysis, DNA
D004247:DNA