Proteomics profiling of interactome dynamics by colocalisation analysis (COLA).
Localisation and protein function are intimately linked in eukaryotes, as proteins are localised to specific compartments where they come into proximity of other functionally relevant proteins. Significant co-localisation of two proteins can therefore be indicative of their functional association. We here present COLA, a proteomics based strategy coupled with a bioinformatics framework to detect protein-protein co-localisations on a global scale. COLA reveals functional interactions by matching proteins with significant similarity in their subcellular localisation signatures. The rapid nature of COLA allows mapping of interactome dynamics across different conditions or treatments with high precision.
Institute of Cancer Research, Division of Cancer Biology, 237 Fulham Road, London SW3 6JB, UK. chris.bakal@icr.ac.uk mardakheh@icr.ac.uk.
Faraz K
FK
Mardakheh
Institute of Cancer Research, Division of Cancer Biology, 237 Fulham Road, London SW3 6JB, UK. chris.bakal@icr.ac.uk mardakheh@icr.ac.uk and Institute of Biomedical Engineering, University of Oxford, Old Road Campus Research Building, Oxford OX3 7DQ, UK.
Heba Z
HZ
Sailem
Institute of Cancer Research, Division of Cancer Biology, 237 Fulham Road, London SW3 6JB, UK. chris.bakal@icr.ac.uk mardakheh@icr.ac.uk.
Sandra
S
Kümper
Institute of Cancer Research, Division of Cancer Biology, 237 Fulham Road, London SW3 6JB, UK. chris.bakal@icr.ac.uk mardakheh@icr.ac.uk and Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA.
Christopher J
http://orcid.org/0000-0003-4712-2737
CJ
Tape
Institute of Cancer Research, Division of Cancer Biology, 237 Fulham Road, London SW3 6JB, UK. chris.bakal@icr.ac.uk mardakheh@icr.ac.uk.
Ryan R
RR
McCully
Institute of Cancer Research, Division of Cancer Biology, 237 Fulham Road, London SW3 6JB, UK. chris.bakal@icr.ac.uk mardakheh@icr.ac.uk.
Angela
A
Paul
Institute of Cancer Research, Division of Cancer Biology, 237 Fulham Road, London SW3 6JB, UK. chris.bakal@icr.ac.uk mardakheh@icr.ac.uk.
Sara
S
Anjomani-Virmouni
Cancer Research UK Manchester Institute, University of Manchester, Wilmslow Road, Manchester M20 4BX, UK.
Claus
http://orcid.org/0000-0002-7841-943X
C
Jørgensen
Institute of Cancer Research, Division of Cancer Biology, 237 Fulham Road, London SW3 6JB, UK. chris.bakal@icr.ac.uk mardakheh@icr.ac.uk.
George
G
Poulogiannis
Institute of Cancer Research, Division of Cancer Biology, 237 Fulham Road, London SW3 6JB, UK. chris.bakal@icr.ac.uk mardakheh@icr.ac.uk.
Christopher J
CJ
Marshall
Institute of Cancer Research, Division of Cancer Biology, 237 Fulham Road, London SW3 6JB, UK. chris.bakal@icr.ac.uk mardakheh@icr.ac.uk.
Chris
http://orcid.org/0000-0002-0413-6744
C
Bakal
D002458:Cell Fractionation; D002460:Cell Line; D002853:Chromatography, Liquid; D016000:Cluster Analysis; D006801:Humans; D042541:Intracellular Space / Q000378:metabolism; D013058:Mass Spectrometry; D011485:Protein Binding; D025941:Protein Interaction Mapping / Q000379:methods*; D060066:Protein Interaction Maps; D021381:Protein Transport; D020543:Proteome; D040901:Proteomics / Q000379:methods; D012680:Sensitivity and Specificity; D013347:Subcellular Fractions
D020543:Proteome