Influence of nanoporous poly(ether imide) particle extracts on human aortic endothelial cells (HAECs).
Accumulated uremic toxins like indoxyl sulphate, hippuric acid and p-cresyl sulphates in renal failure patients stimulate proinflammatory effects, and consequently kidney and cardiovascular diseases. Low clearance rate of these uremic toxins from the blood of uremic patients by conventional techniques like hemodialysis is due to their strong covalent albumin binding (greater than 95%) and hydrophobic nature, which led to alternatives like usage of hydrophobic adsorber's in removing these toxins from the plasma of kidney patients. Polymers like polyethylene, polyurethane, polymethylmethacrylate, cellophane and polytetrafluoroethylene were already in use as substitutes for metal devices as dialysis membranes. Among new synthetic polymers, one such ideal adsorber material are highly porous microparticles of poly(ether imide) (PEI) with diameters in the range from 50-180μm and a porosity around 88±2% prepared by a spraying and coagulation process.It is essential to make sure that these synthetic polymers should not evoke any inflammatory or apoptotic response during dialysis. Therefore in our study we evaluated in vitro effect of PEI microparticle extracts in human aortic endothelial cells (HEACs) concerning toxicity, inflammation and apoptosis. No cell toxicity was observed when HAECs were treated with PEI extracts and inflammatory/apoptotic markers were not upregulated in presence of PEI extracts. Our results ensure biocompatibility of PEI particles and further hemocompatibility of particles will be tested.
Department of Plant Sciences, School of Life Sciences, University of Hyderabad, Hyderabad, India.
Reddi K
RK
Kumar
Institute of Biomaterial Science and Berlin-Brandenburg Center for Regenerative Therapies, Helmholtz-Zentrum Geesthacht, Teltow, Germany.
Sayantani
S
Basu
eXcorLab GmbH, Industrie Center Obernburg, Obernburg, Germany.
Horst-Dieter
HD
Lemke
Institute for Molecular Cardiovascular Research (IMCAR), RTWTH Aachen University, University Hospital, Pauwelsstraβe 30, Aachen, Germany.|School of Cardiovascular Diseases (CARIM), University of Maastricht, Maastricht, Universiteitssingel 50, The Netherlands.
Joachim
J
Jankowski
Institute of Biomaterial Science and Berlin-Brandenburg Center for Regenerative Therapies, Helmholtz-Zentrum Geesthacht, Teltow, Germany.
Karl
K
Kratz
Institute of Biomaterial Science and Berlin-Brandenburg Center for Regenerative Therapies, Helmholtz-Zentrum Geesthacht, Teltow, Germany.|Institute of Chemistry, University Potsdam, Potsdam, Germany.
Andreas
A
Lendlein
Department of Plant Sciences, School of Life Sciences, University of Hyderabad, Hyderabad, India.
Sarada D
SD
Tetali
D042783:Endothelial Cells / Q000378:metabolism*; D004986:Ether / Q000378:metabolism*; D006801:Humans; D007094:Imides / Q000378:metabolism*; D011108:Polymers / Q000378:metabolism*; D051436:Renal Insufficiency, Chronic / Q000235:genetics* / Q000378:metabolism*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism*
D007094:Imides; D011108:Polymers; D014409:Tumor Necrosis Factor-alpha; D004986:Ether
Poly(ether imide) (PEI); Tumor necrosis factor-alpha(TNF-α); apoptosis; biomaterial; inflammation; medical devices