Retrograde Oxygen Persufflation of Kidney - Experiment on an Animal.
OBJECTIVE
There is still a lack of organs for transplantation purposes. In the field of kidney and liver transplantation, one available solution is the use of organs from so-called marginal donors. These donors can be e.g. non-heart-beating donors. In these cases, perfusion and preservation of organs intended for transplantation is generally more difficult. Retrograde oxygen persufflation (ROP) may be a possible solution to this issue. This method is based on retrograde perfusion by oxygen through the renal vein thus reconditioning the organ.


METHODS
We operated on 10 animals (porcine models). Ischemic injury of the right kidney was simulated in all animals. In group A (N=5), kidneys were perfused with retrograde oxygen persufflation after explantation. In group B (N=5), kidneys were perfused intrarterially as in usual clinical practice. After perfusion all kidneys were transplanted to the original donor animal. Quality of graft restitution was evaluated by the urea level obtained from the renal vein and by histopathological analysis after explantation.


RESULTS
We found no statistically significant differences between groups A and B in urea levels after transplantation, nor did we find any significant differences in quality of kidney parenchyma restoration between these groups.


CONCLUSIONS
Retrograde oxygen persufflation is able to protect and restore kidney parenchyma.
Department of Vascular Surgery, Faculty of Medicine in Pilsen, Charles University, Pilsen, Czech Republic molacek@fnplzen.cz.|Department of Surgery, University Hospital in Pilsen, Pilsen, Czech Republic.
Jiri
J
Moláček
Department of Vascular Surgery, Faculty of Medicine in Pilsen, Charles University, Pilsen, Czech Republic.
Václav
V
Opatrný
Department of Biomedical Technology, Faculty of Biomedical Engineering in Kladno, Czech Technical University in Prague, Kladno, Czech Republic.
Roman
R
Matějka
Department of Surgery, University Hospital in Pilsen, Pilsen, Czech Republic.
Jan
J
Baxa
Department of Vascular Surgery, Faculty of Medicine in Pilsen, Charles University, Pilsen, Czech Republic.|Department of Surgery, University Hospital in Pilsen, Pilsen, Czech Republic.
Vladislav
V
Třeška
D000818:Animals; D007668:Kidney / Q000378:metabolism; D016030:Kidney Transplantation / Q000379:methods*; D008297:Male; D009926:Organ Preservation / Q000379:methods*; D010100:Oxygen / Q000378:metabolism*; D010477:Perfusion / Q000379:methods*; D011169:Portal Vein; D015427:Reperfusion Injury / Q000378:metabolism; D034421:Sus scrofa; D014182:Transplantation, Autologous
D010100:Oxygen
Retrograde oxygen persifflation; kidney transplantation; non-heart-beating donor