Leonurine ameliorates cognitive dysfunction via antagonizing excitotoxic glutamate insults and inhibiting autophagy.
BACKGROUND
Chronic cerebral hypoperfusion is related with cognitive deficits in different types of dementia.


OBJECTIVE
In this study, we aimed to investigate the effect and potential mechanisms of leonurine on chronic cerebral hypoperfusion both in vitro and in vivo.


METHODS
Chronic cerebral hypoperfusion was duplicated by oxygen-glucose deprivation (OGD) in vitro and by ligation of bilateral common carotid arteries (2-VO) in vivo.


METHODS
In in vitro study, there were control group, OGD group, OGD+ 100µM leonurin group, and OGD+ 10µM donepezil group. The spontaneous excitatory postsynaptic current amplitude and frequency were recorded. In in vivo study, the chronic cerebral hypoperfusion model was induced by ligated bilateral common carotid arteries. Rats were randomly divided into Sham group, 2-VO group, 2-VO+ 60mg/kg/day leonurine group, and 2-VO+ 4mg/kg/day donepezil group. After three weeks, the Morris water maze and Long-term depression recording were observed. Then N-methyl-D-aspartate receptor-associated proteins and autophagy-associated proteins were detected by Western blot assay.


RESULTS
In in vitro experiment, results showed that leonurine could obviously attenuate the spontaneous excitatory postsynaptic current amplitude and frequency on pyramidal neurons. In in vivo experiment, leonurine significantly decreased levels of glutamate and hydrogen peroxide, improved both the cognitive flexibility and the spatial learning and memory abilities. Moreover, leonurine obviously enhanced long-term depression, elevated the ratio of N-methyl-D-aspartate receptor 2A/2B, and decreased the expression of postsynaptic density protein-95. Interestingly, the ratio of LC3II/LC3I and beclin-1 expression were markedly down-regulated by leonurine.


CONCLUSIONS
These findings suggest that leonurine ameliorates cognitive dysfunction at least partly via antagonizing excitotoxic glutamate insults and inhibiting autophagy. Furthermore, it might become a potential drug candidate of chronic cerebral hyperfusion in future.
School of Medicine, State Key Laboratory of Medicinal Chemical Biology, Key Laboratory of Bioactive Materials Ministry of Education, Nankai University, Tianjin 300071, China.
Chunhua
C
Liu
School of Medicine, State Key Laboratory of Medicinal Chemical Biology, Key Laboratory of Bioactive Materials Ministry of Education, Nankai University, Tianjin 300071, China.
Hongqiang
H
Yin
School of Medicine, State Key Laboratory of Medicinal Chemical Biology, Key Laboratory of Bioactive Materials Ministry of Education, Nankai University, Tianjin 300071, China.
Jing
J
Gao
College of Life Sciences, Nankai University, Tianjin 300071, China.
Xiaxia
X
Xu
College of Life Sciences, Nankai University, Tianjin 300071, China.
Tao
T
Zhang
School of Medicine, State Key Laboratory of Medicinal Chemical Biology, Key Laboratory of Bioactive Materials Ministry of Education, Nankai University, Tianjin 300071, China. Electronic address: zhuoyang@nankai.edu.cn.
Zhuo
Z
Yang
D000818:Animals; D001343:Autophagy / Q000187:drug effects*; D001921:Brain / Q000098:blood supply / Q000187:drug effects / Q000503:physiopathology; D060825:Cognitive Dysfunction / Q000188:drug therapy* / Q000473:pathology; D004195:Disease Models, Animal; D019706:Excitatory Postsynaptic Potentials / Q000187:drug effects; D005707:Gallic Acid / Q000031:analogs & derivatives* / Q000494:pharmacology; D005971:Glutamates / Q000378:metabolism*; D006624:Hippocampus / Q000187:drug effects / Q000378:metabolism; D018696:Neuroprotective Agents / Q000494:pharmacology*; D017966:Pyramidal Cells / Q000187:drug effects; D017208:Rats, Wistar; D016194:Receptors, N-Methyl-D-Aspartate / Q000378:metabolism; D065852:Spatial Memory / Q000187:drug effects
D005971:Glutamates; D018696:Neuroprotective Agents; D016194:Receptors, N-Methyl-D-Aspartate; C013587:leonurine; D005707:Gallic Acid
Autophagy; Chronic cerebral hypofusion; Hippocampus; Leonurine; Long-term depression