Effect of Hydrofluoric Acid Etching Time on Titanium Topography, Chemistry, Wettability, and Cell Adhesion.
Titanium implant surface etching has proven an effective method to enhance cell attachment. Despite the frequent use of hydrofluoric (HF) acid, many questions remain unresolved, including the optimal etching time and its effect on surface and biological properties. The objective of this study was to investigate the effect of HF acid etching time on Ti topography, surface chemistry, wettability, and cell adhesion. These data are useful to design improved acid treatment and obtain an improved cell response. The surface topography, chemistry, dynamic wetting, and cell adhesiveness of polished Ti surfaces were evaluated after treatment with HF acid solution for 0, 2; 3, 5, 7, or 10 min, revealing a time-dependent effect of HF acid on their topography, chemistry, and wetting. Roughness and wetting increased with longer etching time except at 10 min, when roughness increased but wetness decreased. Skewness became negative after etching and kurtosis tended to 3 with longer etching time. Highest cell adhesion was achieved after 5-7 min of etching time. Wetting and cell adhesion were reduced on the highly rough surfaces obtained after 10-min etching time.
Prosthodontics Department, School of Dentistry, University of Granada, Granada, Spain.|Stomatognatic Physiopathology and Prosthodontics Research Group (CTS-974), University of Granada, Granada, Spain.
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Zahran
Prosthodontics Department, School of Dentistry, University of Granada, Granada, Spain.|Stomatognatic Physiopathology and Prosthodontics Research Group (CTS-974), University of Granada, Granada, Spain.|Instituto de Investigación Biosanitariaibs.GRANADA. University of Granada, Granada, Spain.
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Rosales Leal
Applied Physics Department.School of Sciences.University of Granada, Granada, Spain.|Fluid and Biocolloid Physics Research Group, University of Granada, Granada, Spain.
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Rodríguez Valverde
Applied Physics Department.School of Sciences.University of Granada, Granada, Spain.|Fluid and Biocolloid Physics Research Group, University of Granada, Granada, Spain.
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Cabrerizo Vílchez
D002448:Cell Adhesion / Q000187:drug effects*; D002460:Cell Line; D006801:Humans; D006858:Hydrofluoric Acid / Q000737:chemistry*; D008422:Materials Testing / Q000379:methods; D008855:Microscopy, Electron, Scanning; D013499:Surface Properties; D014025:Titanium / Q000737:chemistry*; D017155:Wettability
D014025:Titanium; D006858:Hydrofluoric Acid