Spatial Organization of Enzyme Cascade on a DNA Origami Nanostructure.
Self-assembled DNA nanostructures hold great promise to organize multi-enzyme systems with the precise control of the geometric arrangements. Enzymes modified with single-stranded DNA anchors are assembled onto the DNA origami tiles by hybridizing with the corresponding complementary strands displayed on the surface of the DNA nanostructures. Here, we describe a protocol of assembling a two-enzyme cascade on a discrete, rectangular DNA origami tile, where the distance between enzymes is precisely controlled for investigating the distance-dependent cascade activities.
Center for Computational and Integrative Biology, Rutgers University, Camden, NJ, 08102, USA. jinglin.fu@rutgers.edu.|Department of Chemistry, Rutgers University, Camden, NJ, 08102, USA. jinglin.fu@rutgers.edu.
Jinglin
J
Fu
Center for Computational and Integrative Biology, Rutgers University, Camden, NJ, 08102, USA.
Tianran
T
Li
D004277:DNA, Single-Stranded / Q000737:chemistry*; D049329:Nanostructures / Q000737:chemistry*; D036103:Nanotechnology / Q000379:methods; D009690:Nucleic Acid Conformation
D004277:DNA, Single-Stranded
AFM; DNA origami; Denaturing PAGE; Enzyme cascade; Protein–DNA conjugation