GoTLR7 but not GoTLR21 mediated antiviral immune responses against low pathogenic H9N2 AIV and Newcastle disease virus infection.
Aquatic birds are considered the biological and genetic reservoirs of avian influenza virus and play a critical role in the transmission and dissemination of Newcastle Disease Virus (NDV). Both TLR7 and TLR21 are important for the host antiviral immune response. In an in vivo study, goTLR7, not goTLR21, was significantly up-regulated in the lungs of geese at 3 to 7 d after challenge with H9N2. And goOASL expression was induced in the bursa of fabricius, harderian glands and lungs. An increase in goRIG-I was detected in the lung and small intestine, whereas goPKR was increased in the lung but decreased in the thymus. In the in vitro study, goTLR7 and goRIG-I but not goTLR21 were highly induced by H9N2. Moreover, goOASL and goPKR were significantly induced in H9N2-treated PBMCs, whereas goMx was suppressed. The over-expression of goTLR7, not goTLR21, controlled NDV replication in DF-1 cells, resulting in a decrease in viral copies and the viral titres. Furthermore, we explored the cellular localization of goTLR7 and goTLR21 in heterologous (DF-1 and BHK21) and homologous cells (GEF) through ectopic expression of goTLRs. The antiviral functions of goTLR7 and goTLR21 during H9N2 and NDV infection and their cellular locations were reported here for the first time. These results will contribute to better understand the TLR-dependent antiviral immune responses of waterfowl.
Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu, Sichuan 611130, China.
Bing
B
Yan
Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu, Sichuan 611130, China.
Jinyue
J
Zhang
Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu, Sichuan 611130, China.
Wei
W
Zhang
Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu, Sichuan 611130, China; Avian Disease Research Center, College of Veterinary Medicine of Sichuan Agricultural University, Chengdu, Sichuan 611130, China; Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Chengdu, Sichuan 611130, China.
Mingshu
M
Wang
Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu, Sichuan 611130, China; Avian Disease Research Center, College of Veterinary Medicine of Sichuan Agricultural University, Chengdu, Sichuan 611130, China; Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Chengdu, Sichuan 611130, China.
Renyong
R
Jia
Avian Disease Research Center, College of Veterinary Medicine of Sichuan Agricultural University, Chengdu, Sichuan 611130, China; Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Chengdu, Sichuan 611130, China.
Dekang
D
Zhu
Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu, Sichuan 611130, China.
Mafeng
M
Liu
Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu, Sichuan 611130, China; Avian Disease Research Center, College of Veterinary Medicine of Sichuan Agricultural University, Chengdu, Sichuan 611130, China; Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Chengdu, Sichuan 611130, China.
Qiao
Q
Yang
Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu, Sichuan 611130, China; Avian Disease Research Center, College of Veterinary Medicine of Sichuan Agricultural University, Chengdu, Sichuan 611130, China; Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Chengdu, Sichuan 611130, China.
Ying
Y
Wu
Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu, Sichuan 611130, China; Avian Disease Research Center, College of Veterinary Medicine of Sichuan Agricultural University, Chengdu, Sichuan 611130, China; Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Chengdu, Sichuan 611130, China.
Kunfeng
K
Sun
Avian Disease Research Center, College of Veterinary Medicine of Sichuan Agricultural University, Chengdu, Sichuan 611130, China; Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Chengdu, Sichuan 611130, China.
Xiaoyue
X
Chen
Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu, Sichuan 611130, China; Avian Disease Research Center, College of Veterinary Medicine of Sichuan Agricultural University, Chengdu, Sichuan 611130, China; Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Chengdu, Sichuan 611130, China. Electronic address: chenganchun@vip.163.com.
Anchun
A
Cheng
Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu, Sichuan 611130, China; Avian Disease Research Center, College of Veterinary Medicine of Sichuan Agricultural University, Chengdu, Sichuan 611130, China; Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Chengdu, Sichuan 611130, China. Electronic address: shunchen@sicau.edu.cn.
Shun
S
Chen
D000818:Animals; D002460:Cell Line; D005777:Geese; D015870:Gene Expression; D054884:Host-Pathogen Interactions / Q000235:genetics / Q000276:immunology; D053127:Influenza A Virus, H9N2 Subtype / Q000276:immunology*; D005585:Influenza in Birds / Q000235:genetics / Q000276:immunology* / Q000378:metabolism* / Q000821:virology; D007372:Interferons / Q000378:metabolism; D009521:Newcastle Disease / Q000235:genetics / Q000276:immunology* / Q000378:metabolism* / Q000821:virology; D009522:Newcastle disease virus / Q000276:immunology*; D021381:Protein Transport; D051199:Toll-Like Receptor 7 / Q000235:genetics / Q000378:metabolism*; D051193:Toll-Like Receptors / Q000235:genetics / Q000378:metabolism*; D019562:Viral Load; D014779:Virus Replication
D051199:Toll-Like Receptor 7; D051193:Toll-Like Receptors; D007372:Interferons
Antiviral immune responses; Cellular localization; GoTLR21; GoTLR7; H9N2; NDV