Evaluating the effects of refined carbohydrate and fat diets with acute ethanol consumption using a mouse model of alcoholic liver injury.
Alcoholism is a multifactorial and complex disorder responsible for 5.9% of deaths worldwide. Excessive consumption of ethanol (Et-OH) induces alcoholic liver disease (ALD), a condition comprising a spectrum of clinical signs and morphological changes, ranging from fatty liver (steatosis) to more severe forms of chronic liver injury. Secondary cofactors, such as nutritional and hepatotoxic comorbid conditions, can also contribute to liver disease development. Here we investigated the effects in the progression of ALD following short-term exposure to diet high in refined carbohydrates (HC), a high-sugar and -butter (HSB) hypercaloric diet and acute Et-OH consumption. HSB diet increased the body weight (BW) and adiposity independently of acute Et-OH consumption. HC diet did not affect BW but increased the adiposity, while acute Et-OH alone did not affect BW and adiposity. All groups of mice developed steatosis except the control group. Exposure to acute Et-OH and HSB diet increased the number of neutrophils and macrophages, and apoptosis in the liver. This combination also increased the number of circulating neutrophils and reduced mononuclear cells in the blood. Thus, short-term exposure to HSB diet and acute Et-OH intake is linked to increased liver injury. These findings offer important clues to understand the hepatic injuries associated with short exposure to hypercaloric diets and acute Et-OH.
Departamento de Biologia Geral, Belo Horizonte; Departamento de Bioquímica e Imunologia, Belo Horizonte.
Juliana L
JL
Gonçalves
Departamento de Biologia Geral, Belo Horizonte; Departamento de Bioquímica e Imunologia, Belo Horizonte.
Norinne
N
Lacerda-Queiroz
Departamento de Bioquímica e Imunologia, Belo Horizonte.
Josiane F L
JFL
Sabino
Departamento de Bioquímica e Imunologia, Belo Horizonte.
Pedro E
PE
Marques
Departamento de Bioquímica e Imunologia, Belo Horizonte.
Izabela
I
Galvão
Departamento de Patologia Geral, Instituto de Ciências Biológicas, Belo Horizonte.
Conrado O
CO
Gamba
Departamento de Patologia Geral, Instituto de Ciências Biológicas, Belo Horizonte.
Geovanni D
GD
Cassali
Departamento de Biologia Geral, Belo Horizonte.
Luana M
LM
de Carvalho
Departamento de Biologia Geral, Belo Horizonte.
Daniel Almeida
DA
da Silva E Silva
Departamento de Nutrição, Escola de Enfermagem, Universidade Federal de Minas Gerais, Belo Horizonte.
Adaliene
A
Versiani
Departamento de Bioquímica e Imunologia, Belo Horizonte.
Mauro M
MM
Teixeira
Departamento de Bioquímica e Imunologia, Belo Horizonte.
Ana Maria Caetano
AMC
de Faria
Departamento de Biologia Geral, Belo Horizonte; Departamento de Bioquímica e Imunologia, Belo Horizonte. Electronic address: angelicathomaz@gmail.com.
Angélica T
AT
Vieira
Departamento de Biologia Geral, Belo Horizonte. Electronic address: brunialt@ufmg.br.
Ana Lúcia
AL
Brunialti-Godard
D050154:Adiposity; D000410:Alanine Transaminase / Q000097:blood; D000428:Alcohol Drinking / Q000009:adverse effects*; D000818:Animals; D001835:Body Weight; D059305:Diet, High-Fat / Q000009:adverse effects*; D004040:Dietary Carbohydrates / Q000008:administration & dosage / Q000009:adverse effects*; D004195:Disease Models, Animal; D005234:Fatty Liver / Q000209:etiology / Q000473:pathology; D005978:Glutathione / Q000097:blood; D008099:Liver / Q000187:drug effects / Q000473:pathology; D008108:Liver Diseases, Alcoholic / Q000209:etiology / Q000473:pathology*; D051379:Mice; D008810:Mice, Inbred C57BL; D009504:Neutrophils / Q000378:metabolism
D004040:Dietary Carbohydrates; D000410:Alanine Transaminase; D005978:Glutathione
Alcoholic liver disease; Diet; Ethanol; Inflammation; Liver; Mice