Multiplex assay development and mutation rate analysis for 13 RM Y-STRs in Chinese Han population.
In this study, a novel multiplex polymerase chain reaction (PCR) assay was developed for amplifying the newly introduced 13 rapidly mutating Y-STR markers (RM Y-STRs) including DYF387S1, DYF399S1, DYF403S1a/b, DYF404S1, DYS449, DYS518, DYS526b, DYS547, DYS570, DYS576, DYS612, DYS626, and DYS627. In addition, a survey for mutation rates of the 13 RM Y-STRs in Chinese Han population was performed to make sure of the mutation characteristic and application in Chinese group. With 14,476 allele transfers in 1034 father-son pairs, 221 mutation events occurred, of which 215 were one-step mutations and 6 were two-step mutations. Nineteen father-son pairs were found to have mutations at two loci and one pair at three loci. Based upon our research data, 18.96 % of all 1034 father-son pairs were successfully differentiated, and the estimated locus-specific mutation rates varied from 4.84 × 10-3to 6.29 × 10-2, with an average estimated mutation rate 1.53 × 10-2(95 % CI 1.33 × 10-2to 1.74 × 10-2). Among the 13 Y-STR markers, eight loci (DYF399S1, DYF403S1a, DYF404S1, DYS449, DYS518, DYS547, DYS576, and DYS612) had mutation rates higher than 1.00 × 10-2, and the rest loci lower than 1.00 × 10-2in Chinese samples.
Department of Forensic Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, People's Republic of China.|Qingdao Licang District People's Procuratorate, Qingdao, 266100, People's Republic of China.
Wenqiong
W
Zhang
Department of Forensic Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, People's Republic of China.
Chao
C
Xiao
Wuhan Blood Center, Wuhan, 430033, People's Republic of China.
Jin
J
Yu
Department of Forensic Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, People's Republic of China.
Tian
T
Wei
Department of Forensic Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, People's Republic of China.
Fei
F
Liao
Department of Forensic Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, People's Republic of China.
Wei
W
Wei
Department of Forensic Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, People's Republic of China. huangdaixin@mails.tjmu.edu.cn.
Daixin
D
Huang
D002681:China; D041322:Chromosomes, Human, Y; D016172:DNA Fingerprinting; D005006:Ethnicity / Q000235:genetics*; D005819:Genetic Markers; D005828:Genetics, Population; D006801:Humans; D008297:Male; D018895:Microsatellite Repeats; D060885:Multiplex Polymerase Chain Reaction; D059645:Mutation Rate
D005819:Genetic Markers
Lineage differentiation; Multiplex assay; Mutation; Rapidly mutating Y-STR