Efficient, Cost-Effective, High-Throughput, Multilocus Sequencing Typing (MLST) Method, NGMLST, and the Analytical Software Program MLSTEZ.
Multilocus sequence typing (MLST) has become the preferred method for genotyping many biological species. It can be used to identify major phylogenetic clades, molecular groups, or subpopulations of a species, as well as individual strains or clones. However, conventional MLST is costly and time consuming, which limits its power for genotyping large numbers of samples. Here, we describe a new MLST method that uses next-generation sequencing, a multiplexing protocol, and appropriate analytical software to provide accurate, rapid, and economical MLST genotyping of 96 or more isolates in a single assay.
Division of Infectious Diseases, Department of Medicine, Duke University Medical Center, Durham, NC, 27710, USA.
Yuan
Y
Chen
Division of Infectious Diseases, Department of Medicine, Duke University Medical Center, Durham, NC, 27710, USA. john.perfect@dm.duke.edu.
John R
JR
Perfect
D003362:Cost-Benefit Analysis; D005838:Genotype; D058885:Multilocus Sequence Typing / Q000379:methods*; D012984:Software
Genotyping; Multilocus sequence typing; Multiplex PCR; Next-generation sequencing