eIF3 Regulation of Protein Synthesis, Tumorigenesis, and Therapeutic Response.
Translation initiation is the rate-limiting step of protein synthesis and highly regulated. Eukaryotic initiation factor 3 (eIF3) is the largest and most complex initiation factor consisting of 13 putative subunits. A growing number of studies suggest that eIF3 and its subunits may represent a new group of proto-oncogenes and associates with prognosis. They regulate translation of a subset of mRNAs involved in many cellular processes including proliferation, apoptosis, DNA repair, and cell cycle. Therefore, unveiling the mechanisms of eIF3 action in tumorigenesis may help identify attractive targets for cancer therapy. Here, we describe a series of methods used in the study of eIF3 function in regulating protein synthesis, tumorigenesis, and cellular response to therapeutic treatments.
Department of Clinical Pharmacology, Xiangya Hospital, Central South University, 110 Xiang Ya Road, Changsha, Hunan, 410078, China. yinjiye@csu.edu.cn.
Ji-Ye
JY
Yin
Department of Pharmacology and Toxicology and IU Simon Cancer Center, Indiana University School of Medicine, 980 W. Walnut Street, Indianapolis, IN, 46202, USA.
Zizheng
Z
Dong
Department of Pharmacology and Toxicology and IU Simon Cancer Center, Indiana University School of Medicine, 980 W. Walnut Street, Indianapolis, IN, 46202, USA. jianzhan@iu.edu.
Jian-Ting
JT
Zhang
D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects; D063646:Carcinogenesis / Q000378:metabolism* / Q000473:pathology; D002470:Cell Survival / Q000187:drug effects; D004354:Drug Screening Assays, Antitumor; D039621:Eukaryotic Initiation Factor-3 / Q000502:physiology*; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D016688:Mice, Inbred NOD; D008819:Mice, Nude; D016513:Mice, SCID; D009368:Neoplasm Transplantation; D011485:Protein Binding; D014176:Protein Biosynthesis; D014407:Tumor Cells, Cultured
D000970:Antineoplastic Agents; D039621:Eukaryotic Initiation Factor-3
Eukaryotic initiation factor 3 (eIF3); Protein synthesis; Therapeutic response; Translational control; Tumorigenesis