Evaluation of the influence of decellularization on the changes in biomechanical properties of primate lungs.
The effect of decellularization on the biomechanical properties of macaque lungs was studied. The quality of the biological scaffold was additionally assessed by morphological methods, and the contents of extracellular matrix (ECM) fibers were determined both qualitatively and quantitatively. Histological analysis revealed no damage of structural integrity of ECM components, but the scaffold elasticity significantly decreased, which was confirmed by the changes in the hysteresis loop without a concomitant decrease in peak loads, with the mechanical strength of the samples being retained. These changes require taking additional measures to prevent a decrease in the effective lung volume.
Kuban State Medical University, Ministry of Healthcare of the Russian Federation, ul. Sedina 4, Krasnodar, 350000, Russia. elenakuevda@yandex.ru.
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Kuevda
Kuban State Medical University, Ministry of Healthcare of the Russian Federation, ul. Sedina 4, Krasnodar, 350000, Russia.
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Gubareva
Kurchatov Institute Russian Research Center, pl. Kurchatova 1, Moscow, 123182, Russia.
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Krasheninnikov
Kurchatov Institute Russian Research Center, pl. Kurchatova 1, Moscow, 123182, Russia.
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Grigoriev
Kuban State Medical University, Ministry of Healthcare of the Russian Federation, ul. Sedina 4, Krasnodar, 350000, Russia.
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Gumenyuk
Kuban State Medical University, Ministry of Healthcare of the Russian Federation, ul. Sedina 4, Krasnodar, 350000, Russia.
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Sotnichenko
Kuban State Medical University, Ministry of Healthcare of the Russian Federation, ul. Sedina 4, Krasnodar, 350000, Russia.
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Gilevich
Research Institute of Medical Primatology, ul. Mira 177, Veseloe village, Adler district, Sochi, 354376, Russia.
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Karal-Ogly
Research Institute of Medical Primatology, ul. Mira 177, Veseloe village, Adler district, Sochi, 354376, Russia.
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Orlov
Kurchatov Institute Russian Research Center, pl. Kurchatova 1, Moscow, 123182, Russia.
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Chvalun
Kuban State Medical University, Ministry of Healthcare of the Russian Federation, ul. Sedina 4, Krasnodar, 350000, Russia.
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Redko
Kuban State Medical University, Ministry of Healthcare of the Russian Federation, ul. Sedina 4, Krasnodar, 350000, Russia.
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Alekseenko
Kuban State Medical University, Ministry of Healthcare of the Russian Federation, ul. Sedina 4, Krasnodar, 350000, Russia.
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Macciarini
D000818:Animals; D001696:Biomechanical Phenomena; D004548:Elasticity; D005109:Extracellular Matrix / Q000378:metabolism*; D008168:Lung / Q000502:physiology*; D008253:Macaca mulatta; D008297:Male; D023822:Tissue Engineering / Q000379:methods; D054457:Tissue Scaffolds