Deletion of a Stay-Green Gene Associates with Adaptive Selection in Brassica napus.
Chlorophyll levels provide important information about plant growth and physiological plasticity in response to changing environments. The stay-green gene NON-YELLOWING 1 (NYE1) is believed to regulate chlorophyll degradation during senescence, concomitantly affecting the disassembly of the light-harvesting complex and hence indirectly influencing photosynthesis. We identified Brassica napus accessions carrying an NYE1 deletion associated with increased chlorophyll content, and with upregulated expression of light-harvesting complex and photosynthetic reaction center (PSI and PSII) genes. Comparative analysis of the seed oil content of accessions with related genetic backgrounds revealed that the B. napus NYE1 gene deletion (bnnye1) affected oil accumulation, and linkage disequilibrium signatures suggested that the locus has been subject to artificial selection by breeding in oilseed B. napus forms. Comparative analysis of haplotype diversity groups (haplogroups) between three different ecotypes of the allopolyploid B. napus and its A-subgenome diploid progenitor, Brassica rapa, indicated that introgression of the bnnye1 deletion from Asian B. rapa into winter-type B. napus may have simultaneously improved its adaptation to cooler environments experienced by autumn-sown rapeseed.
Department of Plant Breeding, IFZ Research Centre for Biosystems, Land Use and Nutrition, Justus Liebig University, Heinrich-Buff-Ring 26-32, 35392 Giessen, Germany.
Lunwen
L
Qian
Department of Plant Breeding, IFZ Research Centre for Biosystems, Land Use and Nutrition, Justus Liebig University, Heinrich-Buff-Ring 26-32, 35392 Giessen, Germany.
Kai
K
Voss-Fels
College of Agronomy and Biotechnology, Southwest University, 400716 Chongqing, China.
Yixin
Y
Cui
Department of Plant Breeding, IFZ Research Centre for Biosystems, Land Use and Nutrition, Justus Liebig University, Heinrich-Buff-Ring 26-32, 35392 Giessen, Germany.
Habib U
HU
Jan
Department of Plant Breeding, IFZ Research Centre for Biosystems, Land Use and Nutrition, Justus Liebig University, Heinrich-Buff-Ring 26-32, 35392 Giessen, Germany.
Birgit
B
Samans
Department of Plant Breeding, IFZ Research Centre for Biosystems, Land Use and Nutrition, Justus Liebig University, Heinrich-Buff-Ring 26-32, 35392 Giessen, Germany.
Christian
C
Obermeier
College of Agronomy and Biotechnology, Southwest University, 400716 Chongqing, China.
Wei
W
Qian
Department of Plant Breeding, IFZ Research Centre for Biosystems, Land Use and Nutrition, Justus Liebig University, Heinrich-Buff-Ring 26-32, 35392 Giessen, Germany. Electronic address: rod.snowdon@agrar.uni-giessen.de.
Rod J
RJ
Snowdon
D029688:Brassica napus / Q000235:genetics* / Q000378:metabolism / Q000502:physiology*; D029741:Brassica rapa / Q000235:genetics / Q000378:metabolism / Q000502:physiology; D001947:Breeding; D002734:Chlorophyll / Q000378:metabolism; D018745:Genome, Plant / Q000235:genetics; D006239:Haplotypes / Q000235:genetics; D010788:Photosynthesis / Q000235:genetics / Q000502:physiology*
D002734:Chlorophyll
PAV; breeding; chlorophyll; photosynthesis; presence-absence variation; rapeseed