Evaluation of Biogas Production Performance and Dynamics of the Microbial Community in Different Straws.
The development and utilization of crop straw biogas resources can effectively alleviate the shortage of energy, environmental pollution, and other issues. This study performed a continuous batch test at 35°C to assess the methane production potential and volatile organic acid contents using the modified Gompertz equation. Illumina MiSeq platform sequencing, which is a sequencing method based on sequencing-by-synthesis, was used to compare the archaeal community diversity, and denaturing gradient gel electrophoresis (DGGE) was used to analyze the bacterial community diversity in rice straw, dry maize straw, silage maize straw, and tobacco straw. The results showed that cumulative gas production values for silage maize straw, rice straw, dry maize straw, and tobacco straw were 4,870, 4,032.5, 3,907.5, and 3,628.3 ml/g ·VS , respectively, after 24 days. Maximum daily gas production values of silage maize straw and rice straw were 1,025 and 904.17 ml/g ·VS, respectively, followed by tobacco straw and dry maize straw. The methane content of all four kinds of straws was > 60%, particularly that of silage maize straw, which peaked at 67.3%. Biogas production from the four kinds of straw was in the order silage maize straw > rice straw > dry maize straw > tobacco straw, and the values were 1,166.7, 1,048.4, 890, and 637.4 ml/g ·VS, respectively. The microbial community analysis showed that metabolism was mainly carried out by acetate-utilizing methanogens, and thatMethanosarcinawas the dominant archaeal genus in the four kinds of straw, and the DGGE bands belonged to the phyla Firmicutes, Bacteroidetes, and Chloroflexi. Silage maize is useful for biogas production because it contains four kinds of straw.
Yanbian University, Yanji, Jilin 133001, P.R. China.
Xue
X
Li
Yanbian University, Yanji, Jilin 133001, P.R. China.
Yan-Hua
YH
Liu
Yanbian University, Yanji, Jilin 133001, P.R. China.
Xin
X
Zhang
Yanbian University, Yanji, Jilin 133001, P.R. China.
Chang-Ming
CM
Ge
Yanbian University, Yanji, Jilin 133001, P.R. China.
Ren-Zhe
RZ
Piao
Heilongjiang August First Land Reclamation University, Daqing, Heilongjiang 163319, P.R. China.
Wei-Dong
WD
Wang
China Agriculture University, Beijing 100083, P.R. China.
Zong-Jun
ZJ
Cui
Yanbian University, Yanji, Jilin 133001, P.R. China.
Hong-Yan
HY
Zhao
D001673:Biodegradation, Environmental; D044822:Biodiversity; D056804:Biofuels; D019149:Bioreactors; D018556:Crops, Agricultural; D004783:Environmental Microbiology; D005285:Fermentation; D054892:Metagenome; D056186:Metagenomics; D008697:Methane / Q000096:biosynthesis; D064307:Microbiota; D055549:Volatile Organic Compounds
D056804:Biofuels; D055549:Volatile Organic Compounds; D008697:Methane
Straw; anaerobic fermentation; microbial community