How to recognize and resolve reagent-dependent reactivity: a review.
Reagent-dependent reactivity can be described as agglutination of red blood cells (RBCs) in serologic testing that is not related to the interaction of RBC antigens and antibodies that the test system is intended to detect. In other words, reagent-dependent reactivity results in false-positive agglutination reactions in serologic testing. These false-positive reactions can cause confusion in antigen typing and RBC antibody detection and identification procedures, and may result in delays in patient transfusion. It is imperative that reagent-dependent reactivity is recognized and resolved during the investigation of ABO discrepancies, positive RBC antibody screens and antibody identification panels, and crossmatch reactivity.
IRL Supervisor.
Gavin C
GC
Patch
IRL Lead Technologist.
Charles F
CF
Hutchinson
IRL Lead Technologist.
Nancy A
NA
Lang
Associate Medical Director, Community Blood Center, 349 South Main St., Dayton, OH 45402.
Ghada
G
Khalife
D000372:Agglutination Tests; D000918:Antibody Specificity; D016477:Artifacts; D001331:Automation; D001788:Blood Grouping and Crossmatching / Q000379:methods; D004912:Erythrocytes / Q000276:immunology*; D005189:False Positive Reactions; D057166:High-Throughput Screening Assays; D006801:Humans; D007106:Immune Sera / Q000276:immunology; D007202:Indicators and Reagents; D007518:Isoantibodies / Q000097:blood*; D011310:Preservatives, Pharmaceutical; D011356:Product Labeling
D007106:Immune Sera; D007202:Indicators and Reagents; D007518:Isoantibodies; D011310:Preservatives, Pharmaceutical