Mulberry water extract regulates the osteoblast/osteoclast balance in an ovariectomic rat model.
Mulberry has favorable antioxidant ability. Menopause is a major cause of osteoporosis in women, and it is partially associated with oxidative stress. Here, mulberry water extract (MWE) was fed to ovariectomic (OVX) rats to explore the potential effect of MWE on osteoporosis. The results revealed that plasma alkaline phosphatase (ALP) significantly decreased in the OVX rats after MWE administration for 8 weeks. Histological examination showed that the MWE increased the density of trabecular bone in the OVX rats. It also revealed that the MWE increases the expression of Runx2 and decreases that of the receptor activator of nuclear factor κB and its ligand in the OVX rats. This implies that MWE might regulate osteoblast differentiation and osteoclast proliferation. The MWE improved the antioxidant status by lowering the expression of heme oxygenase-1. In addition, the MWE increased the expression of osteocalcin, ALP, and bone morphogenetic protein-2 in an osteoblast cell line, MG-63. The present results imply that MWE has potential to decelerate osteoporosis in an experimental OVX rat model.
Institute of Biochemistry, Microbiology and Immunology, Medical College, Chung Shan Medical University, Taichung, Taiwan.
Hsing-Yu
HY
Jao
Department of Pathology, Chung Shan Medical University Hospital, Taichung, Taiwan.
Jeng-Dong
JD
Hsu
Institute of Biochemistry, Microbiology and Immunology, Medical College, Chung Shan Medical University, Taichung, Taiwan and Department of Pathology, Chung Shan Medical University Hospital, Taichung, Taiwan.
Yi-Ru
YR
Lee
Department of Orthopaediology, Show Chwuan Memory Hospital, Changhua, Taiwan. johnlcs317@gmail.com.
Chien-Sheng
CS
Lo
Institute of Biochemistry, Microbiology and Immunology, Medical College, Chung Shan Medical University, Taichung, Taiwan and Department of Biochemistry, School of Medicine, College of Medicine, Chung Shan Medical University, Taichung, Taiwan and Department of Clinical Biochemistry, Chung Shan Medical University Hospital, Taichung, Taiwan. lhj@csmu.edu.tw.
Huei-Jane
HJ
Lee
D000818:Animals; D002460:Cell Line; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects; D031625:Morus / Q000737:chemistry*; D010006:Osteoblasts / Q000187:drug effects; D010052:Ovariectomy; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D014867:Water / Q000737:chemistry*
D010936:Plant Extracts; D014867:Water