Suppression of isoproterenol-induced apoptosis in H9c2 cardiomyoblast cells by daidzein through activation of Akt.
Increased serum norepinephrine level is one of pathological processes relating to heart disease (HD). Estrogens
are considered as potential therapeutics for the treatment of HD; however, estrogen supplementation
shows some side-effects, such as increasing the risk of developing breast, endometrial and ovarian cancers.
This study investigated the cardio-protective effects of daidzein (Dai), a selective estrogen receptor
modulator (SERM) from soy bean extract, in H9c2 cardiomyoblast cells treated with isoproterenol (ISO),
a norepinephrine analog. In this in vitro model, H9c2 cells treated with Dai at different concentrations
showed no statistical difference in cell viability. TdT-mediated digoxigenin-dUTP nick-end labeling (TUNEL) data and western blotting results indicated
that Dai treated-H9c2 cells recovered from the damage induced by ISO. The recovery effects of Dai
on ISO-induced damage were blocked by inhibition of Akt activation through adding Akt inhibitor.
On the other hand, the fold changes of phosphorylated Akt (p-Akt)/Akt normalized with the control for con, 0.25, 0.5, 1, 3
and 24 h of treatment were 1, 2, 5, 13, 11 and 10, respectively. In conclusion, Dai ameliorates apoptosis
of cardiomyoblasts induced by ISO through Akt signaling pathway.
School of Medicine, College of Medicine, China Medical University, Taichung 40402, Republic of China.|Division of Cardiovascular Medicine, Department of Medicine, China Medical University Hospital, Taichung 40447, Republic of China.
Wei-Syun
WS
Hu
Department of Pathology, Changhua Christian Hospital, Changhua 50006, Republic of China.|Jen-Teh Junior College of Medicine, Nursing and Management, Miaoli 35664, Republic of China.
Yueh-Min
YM
Lin
Department of Biological Science and Technology, China Medical University, Taichung 40402, Republic of China.
Wei-Wen
WW
Kuo
Division of Cardiology, Armed Force Taichung General Hospital, Taichung 41152, Republic of China.|Department of Medical Imaging and Radiological Sciences of Central Taiwan University of Science and Technology, Taichung 40601, Republic of China.
Lung-Fa
LF
Pan
Department of Pathology, Changhua Christian Hospital, Changhua 50006, Republic of China.|Jen-Teh Junior College of Medicine, Nursing and Management, Miaoli 35664, Republic of China.
Yu-Lan
YL
Yeh
Graduate Institute of Basic Medical Science, China Medical University, Taichung 40402, Republic of China.
Yi-Hui
YH
Li
Department of Sports Sciences, University of Taipei, Taipei 10048, Republic of China.
Chia-Hua
CH
Kuo
Department of Surgery, School of Medicine, College of Medicine, Taipei Medical University, Taipei 11031, Republic of China.
Ray-Jade
RJ
Chen
Department of Biotechnology, Bharathiar University, Coimbatore-641 046, India.
V. Vijaya
VV
Padma
Graduate Institute of Basic Medical Science, China Medical University, Taichung 40402, Republic of China.|Biomaterials Translational Research Center, China Medical University Hospital, Taichung 40402, Republic of China.
Tung-Sheng
TS
Chen
Graduate Institute of Basic Medical Science, China Medical University, Taichung 40402, Republic of China.|Graduate Institute of Chinese Medical Science, China Medical University, Taichung 40402, Republic of China.|Department of Health and Nutrition Biotechnology, Asia University, Taichung 41354, Taiwan, Republic of China.
Chih-Yang
CY
Huang
D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D002460:Cell Line; D020287:In Situ Nick-End Labeling; D007529:Isoflavones / Q000494:pharmacology*; D007545:Isoproterenol; D032386:Myoblasts, Cardiac / Q000187:drug effects* / Q000378:metabolism; D048789:Phytoestrogens / Q000494:pharmacology*; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism*; D051381:Rats
D007529:Isoflavones; D048789:Phytoestrogens; C004742:daidzein; D051057:Proto-Oncogene Proteins c-akt; D007545:Isoproterenol
apoptosis; daidzein; estrogen; heart disease; isoproterenol