Follicular fluid lipid peroxidation levels in women with endometriosis during controlled ovarian hyperstimulation.
This observational study aimed to establishing a relationship between lipid peroxidation and endometriosis in women undergoing controlled ovarian hyperstimulation. A total of 79 women were divided into two groups: (i) controls (tubal or male factor); and (ii) endometriosis (stages III/IV). The endometriosis diagnosis was confirmed by videolaparoscopy and the controlled ovarian stimulation protocol was similar to all patients. Follicular fluid (FF) lipid peroxidation levels were determined through the quantification of malondialdehyde. Statistical analysis was performed using parametric and non-parametric tests, logistic regression was performed to estimate the chance of achieving a pregnancy in each group and a moving average was calculated for the endometriosis group. Peroxidation levels in the endometriosis group were significantly higher when compared to controls. The moving average showed a decrease of MDA levels in the endometriosis group with increasing female age. Moreover, women with endometriosis who were under 33 years of age were 4.3 times more likely to achieve a pregnancy than women above that age. In conclusion, endometriosis is associated with increased FF oxidative stress (OS) in patients undergoing in vitro fertilization (IVF). Also, increasing age is associated with a decrease in severity of the oxidative status, but a decreased chance of pregnancy.
a Department of Surgery, Division of Urology, Human Reproduction Section , Sao Paulo Federal University, Sao Paulo Hospital , Sao Paulo , Brazil.
Camila Bruna
CB
de Lima
a Department of Surgery, Division of Urology, Human Reproduction Section , Sao Paulo Federal University, Sao Paulo Hospital , Sao Paulo , Brazil.
Fernanda Bertuccez
FB
Cordeiro
a Department of Surgery, Division of Urology, Human Reproduction Section , Sao Paulo Federal University, Sao Paulo Hospital , Sao Paulo , Brazil.
Mariana
M
Camargo
a Department of Surgery, Division of Urology, Human Reproduction Section , Sao Paulo Federal University, Sao Paulo Hospital , Sao Paulo , Brazil.
Daniel Suslik
DS
Zylbersztejn
a Department of Surgery, Division of Urology, Human Reproduction Section , Sao Paulo Federal University, Sao Paulo Hospital , Sao Paulo , Brazil.
Agnaldo Pereira
AP
Cedenho
a Department of Surgery, Division of Urology, Human Reproduction Section , Sao Paulo Federal University, Sao Paulo Hospital , Sao Paulo , Brazil.
Ricardo Pimenta
RP
Bertolla
a Department of Surgery, Division of Urology, Human Reproduction Section , Sao Paulo Federal University, Sao Paulo Hospital , Sao Paulo , Brazil.
Edson Guimarães
EG
Lo Turco
D000328:Adult; D000375:Aging; D006063:Chorionic Gonadotropin / Q000008:administration & dosage; D004624:Embryo Transfer; D004715:Endometriosis / Q000150:complications*; D005260:Female; D005300:Fertility Agents, Female / Q000008:administration & dosage*; D005640:Follicle Stimulating Hormone / Q000008:administration & dosage; D015571:Follicular Fluid / Q000737:chemistry*; D007987:Gonadotropin-Releasing Hormone / Q000008:administration & dosage / Q000031:analogs & derivatives; D006728:Hormones / Q000008:administration & dosage; D006801:Humans; D015227:Lipid Peroxidation; D016015:Logistic Models; D007986:Luteinizing Hormone; D008315:Malondialdehyde / Q000737:chemistry / Q000378:metabolism; D010062:Ovulation Induction; D011247:Pregnancy; D017392:Thiobarbituric Acid Reactive Substances
D006063:Chorionic Gonadotropin; D005300:Fertility Agents, Female; D006728:Hormones; D017392:Thiobarbituric Acid Reactive Substances; D007987:Gonadotropin-Releasing Hormone; D008315:Malondialdehyde; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone; C062876:cetrorelix
Endometriosis; TBARS; follicular fluid; oxidative stress