Positive Regulation of Decidualization by l-Type Amino Acid Transporter 1 (lat1) in Pregnant Mice.
Amino acids have an important role in the pre and post implantation of placenta and embryo development. l-type amino-acid transporter 1 (lat1) is responsible for the transportation of large neutral amino acids and is mainly expressed in human fetal liver, placenta, brain, etc. This study is the first to investigate the expression of lat1 in the early pregnancy of mouse uteri and its role in the process of decidualization. Endometrial stromal cells of a mouse model were used to evaluate decidualization from Day 4-8 of pregnancy in vitro followed by lat1 knock down by small interfering RNA and by a competitive inhibitor of Leucine transport 2-aminobicyclo-(2,2,1)-heptane-2-carboxylic acid (BCH). The effects of lat1 on decidualization in vivo were assessed by injecting BCH into the uterine horns. The mRNA and protein expressions of lat1 in the implantation sites were higher than that in the inter-implantation sites and were localized in the luminal and gland epithelium, stromal and decidual cells. Its increased expression (p< 0.05) was associated with artificial decidualization as well as activation of prl expression. Down-regulation of lat1 expression in these cells by siRNA and BCH inhibited the decidual progression in vitro. Inhibition of lat1 transportation by BCH controlled decidual progression in vivo also accompanied the down-regulation of prl, lat1 expression in the decidual area and embryo size on Day 8 of pregnancy. In conclusion, these results revealed that lat1 might play an important role in the decidual progression both in vitro and in vivo.
Laboratory Animal Center, Chongqing Medical University, Chongqing 400016, China. duomiaicha@163.com.
Xiaojie
X
Wang
Laboratory Animal Center, Chongqing Medical University, Chongqing 400016, China. dongmei_tan@126.com.
Dongmei
D
Tan
Laboratory Animal Center, Chongqing Medical University, Chongqing 400016, China. mj5658700@126.com.
Jing
J
Ma
Laboratory Animal Center, Chongqing Medical University, Chongqing 400016, China. lianghaowd@163.com.
Hao
H
Liang
Laboratory Animal Center, Chongqing Medical University, Chongqing 400016, China. jenniferzhang85@126.com.
Qian
Q
Zhang
Laboratory Animal Center, Chongqing Medical University, Chongqing 400016, China. tanyee66@hotmail.com.
Yi
Y
Tan
Chong Qing Obstetrics and Gynecology Hospital, Chongqing 400013, China. leidoug@gmail.com.
Jiang
J
Wang
Chongqing Key Laboratory of Oral Diseases and Biomedical Sciences, Chongqing 401147, China. lwplucky@163.com.|Stomatological Hospital of Chongqing Medical University, Chongqing 401147, China. lwplucky@163.com.|Chongqing Municipal Key Laboratory of Oral Biomedical Engineering of Higher Education, Chongqing 401147, China. lwplucky@163.com.
Wenping
W
Luo
D027182:Amino Acid Transport System y+ / Q000037:antagonists & inhibitors / Q000235:genetics / Q000502:physiology*; D027241:Amino Acid Transport System y+L; D000598:Amino Acids, Cyclic / Q000494:pharmacology; D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D002478:Cells, Cultured; D003656:Decidua / Q000502:physiology*; D015536:Down-Regulation / Q000187:drug effects; D010064:Embryo Implantation / Q000502:physiology; D047108:Embryonic Development / Q000502:physiology; D005260:Female; D015870:Gene Expression; D005865:Gestational Age; D007930:Leucine / Q000378:metabolism; D051379:Mice; D011247:Pregnancy; D012333:RNA, Messenger / Q000032:analysis; D034741:RNA, Small Interfering / Q000494:pharmacology; D017154:Stromal Cells; D014599:Uterus / Q000378:metabolism
D027182:Amino Acid Transport System y+; D027241:Amino Acid Transport System y+L; D000598:Amino Acids, Cyclic; D012333:RNA, Messenger; D034741:RNA, Small Interfering; C518287:Slc7a7 protein, mouse; C006504:2-aminobicyclo(2,2,1)heptane-2-carboxylic acid; D007930:Leucine
">l-amino acid transporter-1 (lat1); decidualization; pregnant mice; stromal cell