Cytogenetic and Molecular Characterization of B-Genome Introgression Lines of Brassica napus L.
Brassica napus introgression lines (ILs), having B-genome segments from B. carinata, were assessed genetically for extent of introgression and phenotypically for siliqua shatter resistance. Introgression lines had 7-9% higher DNA content, were meiotically stable, and had almost normal pollen fertility/seed set. Segment introgressions were confirmed by fluorescent genomic in situ hybridization (fl-GISH), SSR analyses, and SNP studies. Genotyping with 48 B-genome specific SSRs detected substitutions from B3, B4, B6, and B7 chromosomes on 39 of the 69 ILs whereas SNP genotyping detected a total of 23 B-segments (≥3 Mb) from B4, B6, and B7 introgressed into 10 of the 19 (C1, C2, C3, C5, C6, C8, C9, A3, A9, A10) chromosomes in 17 ILs. The size of substitutions varied from 3.0 Mb on chromosome A9 (IL59) to 42.44 Mb on chromosome C2 (IL54), ranging from 7 to 83% of the recipient chromosome. Average siliqua strength in ILs was observed to be higher than that of B. napus parents (2.2-6.0 vs. 1.9-4.0 mJ) while siliqua strength in some of the lines was almost equal to that of the donor parent B. carinata (6.0 vs.7.2 mJ). These ILs, with large chunks of substituted B-genome, can prove to be a useful prebreeding resource for germplasm enhancement in B. napus, especially for siliqua shatter resistance.
Department of Plant Breeding and Genetics, Punjab Agricultural University, Ludhiana 141001, India.
Inderpreet
I
Dhaliwal
School of Agriculture and Food Sciences, University of Queensland, Brisbane, Queensland 4072, Australia.|Department of Plant Breeding, Research Centre for Biosystems, Land Use and Nutrition, Justus Liebig University, 35392 Giessen, Germany.
Annaliese S
AS
Mason
Department of Plant Breeding and Genetics, Punjab Agricultural University, Ludhiana 141001, India.
Shashi
S
Banga
Department of Plant Breeding and Genetics, Punjab Agricultural University, Ludhiana 141001, India.
Sakshi
S
Bharti
Department of Plant Breeding and Genetics, Punjab Agricultural University, Ludhiana 141001, India.
Beerpal
B
Kaur
Institute of Land and Food Resources, University of Melbourne, Parkville 3010, Victoria, Australia.
Allison Mary
AM
Gurung
Institute of Land and Food Resources, University of Melbourne, Parkville 3010, Victoria, Australia.
Phillip Anthony
PA
Salisbury
School of Agriculture and Food Sciences, University of Queensland, Brisbane, Queensland 4072, Australia.|School of Plant Biology, University of Western Australia, Crawley, Western Australia 6009, Australia.
Jacqueline
J
Batley
Department of Plant Breeding and Genetics, Punjab Agricultural University, Ludhiana 141001, India nppbg@pau.edu.
Surinder Singh
SS
Banga
D029688:Brassica napus / Q000235:genetics*; D032461:Chromosomes, Plant / Q000235:genetics; D018745:Genome, Plant / Q000235:genetics*; D005838:Genotype; D006824:Hybridization, Genetic; D017404:In Situ Hybridization, Fluorescence; D010641:Phenotype; D000069600:Plant Breeding; D011003:Ploidies; D020641:Polymorphism, Single Nucleotide / Q000235:genetics
SNP genotyping; fl-GISH; graphical genotyping; oilseed rape; siliqua shatter resistance