Molecular Cloning, Characterization, and mRNA Expression of Hemocyanin Subunit in Oriental River PrawnMacrobrachium nipponense.
Hemocyanin is a copper-containing protein with immune function against disease. In this study, a hemocyanin subunit named MnHc-1 was cloned fromMacrobrachium nipponense. The full-length cDNA of MnHc-1 was 2,163 bp with a 2,028-bp open reading frame (ORF) encoding a polypeptide of 675 amino acids. The MnHc-1 mRNA was expressed in the hepatopancreas, gill, hemocytes, intestine, ovary, and stomach, with the highest level in the hepatopancreas. In the infection trial, the MnHc-1 mRNA transcripts in the hemocytes were significantly downregulated at 3 h after injection ofAeromonas hydrophilaand then upregulated at 6 h and 12 h, followed by a gradual recovery from 24 to 48 h. The MnHc-1 transcriptional expression in the hepatopancreas was measured afterM. nipponensewere fed seven diets with 2.8, 12.2, 20.9, 29.8, 43.1, 78.9, and 157.1 mg Cu kg-1for 8 weeks, respectively. The level of MnHc-1 mRNA was significantly higher in the prawns fed 43.1-157.1 mg Cu kg-1diet than in that fed 2.8-29.8 mg Cu kg-1diet. This study indicated that the MnHc-1 expression can be affected by dietary copper and the hemocyanin may potentially participate in the antibacterial defense ofM. nipponense.
School of Life Sciences, East China Normal University, Shanghai 200062, China; School of Life Sciences, Huzhou University, Huzhou, Zhejiang 313000, China.
Youqin
Y
Kong
School of Life Sciences, East China Normal University, Shanghai 200062, China.
Liqiao
0000-0003-0138-7828
L
Chen
School of Life Sciences, East China Normal University, Shanghai 200062, China; School of Life Sciences, Huzhou University, Huzhou, Zhejiang 313000, China.
Zhili
Z
Ding
School of Biological Sciences, Flinders University, Adelaide, SA 5001, Australia.
Jianguang
0000-0003-2448-8058
J
Qin
School of Life Sciences, East China Normal University, Shanghai 200062, China.
Shengming
S
Sun
School of Life Sciences, East China Normal University, Shanghai 200062, China.
Ligai
L
Wang
School of Life Sciences, Huzhou University, Huzhou, Zhejiang 313000, China.
Jinyun
J
Ye