[Effect of respiratory syncytial virus-related pulmonary infection on endogenous metabolites in large intestinal mucosa in mice].
OBJECTIVE
To investigate the effect of respiratory syncytial virus (RSV)-related pulmonary infection on endogenous metabolites in large intestinal mucosa in BALB/c mice using metabolomics technology based on gas chromatography-mass spectrometry (GC-MS).


METHODS
Mice were randomly divided into a control group and a RSV pneumonia model group (n=16 each). The mouse model of RSV pneumonia was established using intranasal RSV infection (100×TCID50, 50 μL/mouse, once a day). After 7 days of intranasal RSV infection, the mice were sacrificed and GC-MS was used to identify endogenous metabolites and measure the changes in their relative content in colon tissue. SMCA-P12.0 software was used to perform principal component analysis (PCA) and orthogonal partial least squares-discriminant analysis (OPLS-DA) for endogenous metabolites in colon tissue. The differentially expressed metabolites in colon tissue were imported into the metabolic pathway platform Metaboanalyst to analyze related metabolic pathways.


RESULTS
PCA and OPLS-DA showed significant differences between the control and RSV pneumonia model groups. A total of 32 metabolites were identified in the colon tissue of the mice with RSV pneumonia. The RSV pneumonia model group had significant increases in the content of leucine, isoleucine, glycine, alanine, arachidonic acid, and lactic acid, which were related to the valine, leucine, isoleucine, arachidonic acid, and pyruvic acid metabolic pathways.


CONCLUSIONS
RSV pneumonia might cause metabolic disorders in the large intestinal tissue in mice.
Institute of Pediatrics, Nanjing University of Chinese Medicine, Nanjing 210023, China. Wscnj@126.com.
Xin
X
Meng
Shou-Chuan
SC
Wang
Jin-Jun
JJ
Shan
Tong
T
Xie
Jian-Ya
JY
Xu
Cun-Si
CS
Shen
D000597:Amino Acids, Branched-Chain / Q000378:metabolism; D000818:Animals; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry; D007413:Intestinal Mucosa / Q000378:metabolism*; D007420:Intestine, Large / Q000378:metabolism* / Q000473:pathology; D008168:Lung / Q000473:pathology; D051379:Mice; D008807:Mice, Inbred BALB C; D011024:Pneumonia, Viral / Q000378:metabolism*; D018357:Respiratory Syncytial Virus Infections / Q000378:metabolism*
D000597:Amino Acids, Branched-Chain