Stromal Cell-Derived Factor 1 Polymorphism in Retinal Vein Occlusion.
BACKGROUND
Stromal cell-derived factor 1 (SDF1) has crucial role in the regulation of angiogenesis and ocular neovascularisation (NV). The purpose of this study was to evaluate the association between SDF1-3'G(801)A polymorphism and NV complications of retinal vein occlusion (RVO).


METHODS
130 patients with RVO (median age: 69.0, range 35-93 years; male/female- 58/72; 55 patients had central RVO, 75 patients had branch RVO) were enrolled in this study. In the RVO group, 40 (30.8%) patients were diagnosed with NV complications of RVO and 90 (69.2%) patients without NVs. The median follow up period was 40.3 months (range: 18-57 months). The SDF1-3'G(801)A polymorphism was detected by PCR-RFLP. Allelic prevalence was related to reference values obtained in the control group consisted of 125 randomly selected, age and gender matched, unrelated volunteers (median age: 68.0, range 36-95 years; male/female- 53/72). Statistical analysis of the allele and genotype differences between groups (RVO patients vs controls; RVO patients with NV vs RVO patients without NV) was determined by chi-squared test. P value of <0.05 was considered statistically significant.


RESULTS
Hardy-Weinberg criteria was fulfilled in all groups. The SDF1-3'G(801)A allele and genotype frequencies of RVO patients were similar to controls (SDF1-3'A allele: 22.3% vs 20.8%; SDF1-3'(801)AA: 5.4% vs 4.8%, SDF1-3'(801)GG: 60.8% vs 63.2%). The frequency of SDF1-3'(801)AA and SDF1-3'(801)GA genotypes, as well as the SDF1-3'(801)A allele frequency were higher in RVO patients with NV versus in patients without NV complication (SDF1-3'(801)AA+AG genotypes: 57.5% vs 31.1%, p = 0.008; SDF1-3'(801)A allele: 35.0% vs 16.7%, p = 0.002) or versus controls (SDF1-3'(801)AA+AG genotypes 57.5% vs 36.8%, p = 0.021; SDF1-3'(801)A allele: 35.0% vs 20.8% p = 0.01). Carrying of SDF1-3'(801)A allele increased the risk of neovascularisation complications of RVO by 2.69 (OR, 95% CI = 1.47-4.93).


CONCLUSIONS
These findings suggest that carrying SDF1-3'(801)A allele plays a role in the development of neovascular complications in retinal vein occlusion.
Department of Ophthalmology, Semmelweis University, Budapest, Hungary.
Andrea
A
Szigeti
Department of Ophthalmology, Semmelweis University, Budapest, Hungary.
Mónika
M
Ecsedy
Department of Ophthalmology, Semmelweis University, Budapest, Hungary.
Miklós
M
Schneider
MTA-SE Lendület Diabetes Research Group, Research Laboratory for Pediatrics and Nephrology of the Hungarian Academy of Sciences and of the Semmelweis University, Budapest, Hungary.
Lilla
L
Lénárt
Department of Ophthalmology, Semmelweis University, Budapest, Hungary.
Balázs
B
Lesch
Department of Ophthalmology, Semmelweis University, Budapest, Hungary.
Zoltán Zsolt
ZZ
Nagy
MTA-SE Lendület Diabetes Research Group, Research Laboratory for Pediatrics and Nephrology of the Hungarian Academy of Sciences and of the Semmelweis University, Budapest, Hungary.
Andrea
A
Fekete
Department of Ophthalmology, Semmelweis University, Budapest, Hungary.
Zsuzsanna
Z
Récsán
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000483:Alleles; D054377:Chemokine CXCL12 / Q000235:genetics*; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D008297:Male; D008875:Middle Aged; D011110:Polymorphism, Genetic; D012170:Retinal Vein Occlusion / Q000235:genetics*
C515255:CXCL12 protein, human; D054377:Chemokine CXCL12