Whole-Genome Sequencing: Manual Library Preparation.
This protocol describes a manual approach for the preparation of genomic DNA libraries suitable for Illumina sequencing. Genomic DNA fragments produced by shearing by sonication are ligated to adaptors and amplified by polymerase chain reaction (PCR). The amplified DNA, separated by size and gel-purified, is suitable for use as template in whole-genome sequencing.
Elaine
E
Mardis
W Richard
WR
McCombie
D004247:DNA / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification* / Q000378:metabolism; D011088:DNA Ligases / Q000378:metabolism; D004586:Electrophoresis; D015723:Gene Library; D023281:Genomics / Q000379:methods*; D016133:Polymerase Chain Reaction; D013010:Sonication
D004247:DNA; D011088:DNA Ligases