The expression of cytokine and its significance for the intervertebral disks of Kazakhs.
BACKGROUND
Proinflammatory cytokine IL-6 and anti-inflammatory cytokine IL-10 are expressed in herniated intervertebral disks of Kazakhs, but their significance is not yet understood.


METHODS
Specimens of herniated lumbar disk were collected from 30 patients with single disk herniation during lumbar discectomy. As a control, 10 specimens were collected postmortem from the cadavers of individuals who did not die from spinal disease. The clinical symptoms in all cases were evaluated preoperation by the JOA index. The expressions of IL-6 and IL-10 in the degenerative disk cells of each specimen were detected using immunohistochemical staining. The amounts of IL-6 and IL-10 in each intervertebral disk were assessed by enzyme-linked immunosorbent assay.


RESULTS
The expression levels of IL-6 and IL-10 were significantly higher in the patient group than in the control group. Statistical analysis showed differences between ethnic Kazakh and Han patients in both the expression of IL-6 and JOA scores. Immunohistochemical staining showed that in the patient group, IL-6 was found in 28 patients and IL-10 was found in 27 patients.


CONCLUSIONS
The high expression of cytokines, such as IL-6 and IL-10, has an important relationship with disk degeneration. Of course, age is another factor in cell apoptosis of degenerative disks. More research is necessary to discover how ethnicity and heredity impact the levels of expressed cytokines.
People's Hospital of Xinjiang Uygur Autonomous Region, Urumqi, China.
Miao
M
Xiaogang
People's Hospital of Changji Hui Autonomous Prefecture, Changji City, China.
Hou
H
Quanshan
People's Hospital of Xinjiang Uygur Autonomous Region, Urumqi, China.
Zhang
Z
Liping
People's Hospital of Xinjiang Uygur Autonomous Region, Urumqi, China.
Habaxi
H
Kaken
D000328:Adult; D000368:Aged; D002681:China; D015331:Cohort Studies; D017586:Diskectomy; D005006:Ethnicity / Q000706:statistics & numerical data; D005260:Female; D006801:Humans; D016753:Interleukin-10 / Q000032:analysis*; D015850:Interleukin-6 / Q000032:analysis*; D007403:Intervertebral Disc / Q000737:chemistry; D055959:Intervertebral Disc Degeneration / Q000097:blood* / Q000453:epidemiology / Q000601:surgery; D007405:Intervertebral Disc Displacement / Q000097:blood* / Q000453:epidemiology / Q000601:surgery; D008297:Male; D008875:Middle Aged; D055815:Young Adult
C508609:IL10 protein, human; C508600:IL6 protein, human; D015850:Interleukin-6; D016753:Interleukin-10
cytokine; ethnicity; intervertebral disk herniation