Embryonic chirality and the evolution of spiralian left-right asymmetries.
The group Spiralia includes species with one of the most significant cases of left-right asymmetries in animals: the coiling of the shell of gastropod molluscs (snails). In this animal group, an early event of embryonic chirality controlled by cytoskeleton dynamics and the subsequent differential activation of the genes nodal and Pitx determine the left-right axis of snails, and thus the direction of coiling of the shell. Despite progressive advances in our understanding of left-right axis specification in molluscs, little is known about left-right development in other spiralian taxa. Here, we identify and characterize the expression of nodal and Pitx orthologues in three different spiralian animals-the brachiopod Novocrania anomala, the annelid Owenia fusiformis and the nemertean Lineus ruber-and demonstrate embryonic chirality in the biradial-cleaving spiralian embryo of the bryozoan Membranipora membranacea We show asymmetric expression of nodal and Pitx in the brachiopod and annelid, respectively, and symmetric expression of Pitx in the nemertean. Our findings indicate that early embryonic chirality is widespread and independent of the cleavage programme in the Spiralia. Additionally, our study illuminates the evolution of nodal and Pitx signalling by demonstrating embryonic asymmetric expression in lineages without obvious adult left-right asymmetries.This article is part of the themed issue 'Provocative questions in left-right asymmetry'.
Sars International Centre for Marine Molecular Biology, University of Bergen, Thormøhlensgate 55, Bergen, 5006, Norway.
José M
0000-0002-2572-1061
JM
Martín-Durán
Sars International Centre for Marine Molecular Biology, University of Bergen, Thormøhlensgate 55, Bergen, 5006, Norway.
Bruno C
0000-0002-0000-9465
BC
Vellutini
Sars International Centre for Marine Molecular Biology, University of Bergen, Thormøhlensgate 55, Bergen, 5006, Norway andreas.hejnol@uib.no.
Andreas
0000-0003-2196-8507
A
Hejnol
D000818:Animals; D005075:Biological Evolution; D019521:Body Patterning; D018507:Gene Expression Regulation, Developmental; D007448:Invertebrates / Q000196:embryology* / Q000235:genetics; D055457:Nodal Protein / Q000235:genetics; D014157:Transcription Factors / Q000235:genetics
D055457:Nodal Protein; D014157:Transcription Factors
Nodal; Pitx; Spiralia; evolution; left–right axis