Library Quantification Using SYBR Green-Quantitative Polymerase Chain Reaction (qPCR).
To quantify complex DNA libraries, Kapa Biosystems engineered a DNA polymerase specifically for SYBR Green-based qPCR, enabling efficient amplification of targets such as GC-rich DNAs that present a challenge to wild-type DNA polymerases. Kapa Library Quantification Kits contain this engineered polymerase to ensure robust amplification of longer fragments, across a broad range of GC content.
Elaine
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Mardis
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D052160:Benzothiazoles; D004247:DNA / Q000032:analysis* / Q000235:genetics*; D003959:Diamines; D015723:Gene Library; D009930:Organic Chemicals / Q000378:metabolism; D011804:Quinolines; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*; D013194:Staining and Labeling / Q000379:methods
D052160:Benzothiazoles; D003959:Diamines; D009930:Organic Chemicals; D011804:Quinolines; C098022:SYBR Green I; D004247:DNA