Adhesion of Porphyromonas gingivalis and Tannerella forsythia to dentin and titanium with sandblasted and acid etched surface coated with serum and serum proteins - An in vitro study.
OBJECTIVE
To evaluate the adhesion of selected bacterial strains incl. expression of important virulence factors at dentin and titanium SLA surfaces coated with layers of serum proteins.


METHODS
Dentin- and moderately rough SLA titanium-discs were coated overnight with human serum, or IgG, or human serum albumin (HSA). Thereafter, Porphyromonas gingivalis, Tannerella forsythia, or a six-species mixture were added for 4h and 24h. The number of adhered bacteria (colony forming units; CFU) was determined. Arg-gingipain activity of P. gingivalis and mRNA expressions of P. gingivalis and T. forsythia proteases and T. forsythia protease inhibitor were measured.


RESULTS
Coating specimens never resulted in differences exceeding 1.1 log10 CFU, comparing to controls, irrespective the substrate. Counts of T. forsythia were statistically significantly higher at titanium than dentin, the difference was up to 3.7 log10 CFU after 24h (p=0.002). No statistically significant variation regarding adhesion of the mixed culture was detected between surfaces or among coatings. Arg-gingipain activity of P. gingivalis was associated with log10 CFU but not with the surface or the coating. Titanium negatively influenced mRNA expression of T. forsythia protease inhibitor at 24h (p=0.026 uncoated, p=0.009 with serum).


CONCLUSIONS
The present findings indicate that: a) single bacterial species (T. forsythia) can adhere more readily to titanium SLA than to dentin, b) low expression of T. forsythia protease inhibitor may influence the virulence of the species on titanium SLA surfaces in comparison with teeth, and c) surface properties (e.g. material and/or protein layers) do not appear to significantly influence multi-species adhesion.
Department of Periodontology, School of Dental Medicine, University of Bern, Freiburgstrasse 7, CH-3010 Bern, Switzerland. Electronic address: sigrun.eick@zmk.unibe.ch.
Sigrun
S
Eick
Department of Periodontology, Faculty of Odontology, Malmö University, Malmö, Sweden.
Christian
C
Kindblom
Department of Microbiology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University in Krakow, Gronostajowa Krakow, Poland.
Danuta
D
Mizgalska
Department of Periodontology, School of Dental Medicine, University of Bern, Freiburgstrasse 7, CH-3010 Bern, Switzerland.
Anna
A
Magdoń
Department of Periodontology, School of Dental Medicine, University of Bern, Freiburgstrasse 7, CH-3010 Bern, Switzerland; Department of Conservative Dentistry and Periodontology, Poznan University of Medical Sciences, Poznan, Poland.
Karolina
K
Jurczyk
Department of Periodontology, School of Dental Medicine, University of Bern, Freiburgstrasse 7, CH-3010 Bern, Switzerland.
Anton
A
Sculean
Department of Periodontology, Faculty of Odontology, Malmö University, Malmö, Sweden.
Andreas
A
Stavropoulos
D018829:Adhesins, Bacterial / Q000378:metabolism; D001422:Bacterial Adhesion / Q000187:drug effects*; D020099:Coated Materials, Biocompatible; D015169:Colony Count, Microbial; D003546:Cysteine Endopeptidases / Q000378:metabolism; D045662:Dental Etching; D003804:Dentin / Q000382:microbiology*; D000080867:Gingipain Cysteine Endopeptidases; D006801:Humans; D007074:Immunoglobulin G; D016966:Porphyromonas gingivalis / Q000201:enzymology / Q000254:growth & development / Q000502:physiology*; D011480:Protease Inhibitors; D012333:RNA, Messenger / Q000378:metabolism; D044967:Serum; D000075462:Serum Albumin, Human / Q000494:pharmacology*; D013499:Surface Properties; D000070436:Tannerella forsythia / Q000201:enzymology / Q000254:growth & development / Q000378:metabolism*; D014025:Titanium
D018829:Adhesins, Bacterial; D020099:Coated Materials, Biocompatible; D000080867:Gingipain Cysteine Endopeptidases; D007074:Immunoglobulin G; D011480:Protease Inhibitors; D012333:RNA, Messenger; D014025:Titanium; D003546:Cysteine Endopeptidases; D000075462:Serum Albumin, Human
Bacterial proteases; Implant surface; Porphyromonas gingivalis; Proteinaceous layer; Tannerella forsythia