Prevalence, and virulence determination of Listeria monocytogenes strains isolated from clinical and non-clinical samples by multiplex polymerase chain reaction.
This study aimed to determine the prevalence, and virulence factors of Listeria monocytogenes isolated from various samples by multiplex polymerase chain reaction (MPCR).
A total of 617 isolates were obtained and MPCR was employed for detection of the inlA, inlC, and inlJ genes.
L. monocytogenes was detected in 46 (7.45%) of the 617 specimens. inlA, inlC, and inlJ were detected in 100%, 76.26%, and 71% isolates, respectively.
This study validated MPCR in the analysis and rapid detection of L. monocytogenes. The role of the genes in pathogenesis of the strains can also be affirmed.
Department of Microbiology, School of Medicine, Iran University of Medical Science, Tehran, IR Iran.
Abazar
A
Pournajaf
Department of Microbiology, School of Medicine, Babol University of Medical Science, Babol, IR Iran.
Ramazan
R
Rajabnia
Department of Microbiology, School of Medicine, Iran University of Medical Science, Tehran, IR Iran.
Mansour
M
Sedighi
Department of Community Medicine, School of Medicine, Dezful University of Medical Sciences, Dezful, IR Iran.
Aziz
A
Kassani
Department of Biostatistics, School of Health Sciences, Mazandaran University of Medical Sciences, Sari, IR Iran.
Vahid
V
Moqarabzadeh
Department of Microbiology, School of Medicine, Urmia University of Medical Science, Uremia, IR Iran.
Lida
L
Lotfollahi
Department of Microbiology, School of Medicine, Golestan University of Medical Science, Gorgan, IR Iran.
Abdollah
A
Ardebilli
Department of Microbiology, School of Medicine, Iran University of Medical Science, International campus, Tehran, IR Iran.
Behzad
B
Emadi
Department of Microbiology, School of Medicine, Iran University of Medical Science, Tehran, IR Iran.
Gholamreza
G
Irajian
D000818:Animals; D000829:Animals, Domestic / Q000382:microbiology; D003611:Dairy Products / Q000382:microbiology; D005260:Female; D005516:Food Microbiology; D005838:Genotype; D006801:Humans; D007492:Iran; D008089:Listeria monocytogenes / Q000235:genetics / Q000302:isolation & purification / Q000472:pathogenicity*; D008460:Meat / Q000382:microbiology; D060885:Multiplex Polymerase Chain Reaction; D015995:Prevalence; D012703:Serotyping; D014774:Virulence / Q000235:genetics*