Effects of elevated carbon dioxide on contraction force and proteome composition of sea urchin tube feet.
This study examined how contraction force and protein profiles of the tube feet of the sea urchin (Pseudocentrotus depressus) were affected when acclimated to 400 (control), 2000 and 10,000μatm CO2for 48days. Acclimation to higher CO2conditions significantly reduced contraction force of the tube feet. Two-dimensional gel electrophoresis showed that eight spots changed in protein volume: six up-regulated and two down-regulated. Using matrix-assisted laser desorption/ionization-quadrupole ion trap-time of flight mass spectrometry, three up-regulated spots (tubulin beta chain, tropomyosin fragment, and actin N-terminal fragment) and two down-regulated spots (actin C-terminal fragment and myosin light chain) were identified. One possible interpretation of the results is that elevated CO2weakened contraction of the tube feet muscle through an alteration of proteome composition, mainly associated with post-translational processing/proteolysis of muscle-related proteins.
Institute for East China Sea Research, Nagasaki University, 1551-7 Taira-machi, Nagasaki 851-2213, Japan; Chumphon Marine Fisheries Research and Development Center, Department of Fisheries, 408 M. 8, Paknam, Muang, Chumphon, Thailand, 86120. Electronic address: bb53512001@ms.nagasaki-u.ac.jp.
Nopparat
N
Nasuchon
Graduate School of Fisheries and Environmental Sciences, Nagasaki University, 1-14 Bunkyo, Nagasaki 852-8521, Japan. Electronic address: hirasaka@nagasaki-u.ac.jp.
Katsuya
K
Hirasaka
Graduate School of Fisheries and Environmental Sciences, Nagasaki University, 1-14 Bunkyo, Nagasaki 852-8521, Japan. Electronic address: kenichi@nagasaki-u.ac.jp.
Kenichi
K
Yamaguchi
Graduate School of Fisheries and Environmental Sciences, Nagasaki University, 1-14 Bunkyo, Nagasaki 852-8521, Japan. Electronic address: jokada@nagasaki-u.ac.jp.
Jiro
J
Okada
Institute for East China Sea Research, Nagasaki University, 1551-7 Taira-machi, Nagasaki 851-2213, Japan. Electronic address: a-ishima@nagasaki-u.ac.jp.
Atsushi
A
Ishimatsu
D000818:Animals; D002245:Carbon Dioxide / Q000494:pharmacology*; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods; D009119:Muscle Contraction / Q000187:drug effects / Q000502:physiology*; D010449:Peptide Mapping; D020543:Proteome / Q000032:analysis* / Q000187:drug effects; D040901:Proteomics / Q000379:methods; D012617:Sea Urchins / Q000187:drug effects / Q000378:metabolism*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods
D020543:Proteome; D002245:Carbon Dioxide
Muscle contraction; Proteomics; Sea urchins; Tube feet