Micro-differential scanning calorimeter for liquid biological samples.
We developed an ultrasensitive micro-DSC (differential scanning calorimeter) for liquid protein sample characterization. This design integrated vanadium oxide thermistors and flexible polymer substrates with microfluidics chambers to achieve a high sensitivity (6 V/W), low thermal conductivity (0.7 mW/K), high power resolutions (40 nW), and well-defined liquid volume (1 μl) calorimeter sensor in a compact and cost-effective way. We further demonstrated the performance of the sensor with lysozyme unfolding. The measured transition temperature and enthalpy change were in accordance with the previous literature data. This micro-DSC could potentially raise the prospect of high-throughput biochemical measurement by parallel operation with miniaturized sample consumption.
Department of Mechanical Engineering, Stony Brook University, Stony Brook, New York 11794, USA.
Shuyu
S
Wang
Department of Mechanical Engineering, Virginia Tech, Blacksburg, Virginia 24061, USA.
Shifeng
S
Yu
AbbVie, Deutschland, 67061 Ludwigshafen, Germany.
Michael S
MS
Siedler
AbbVie Bioresearch Center, Worcester, Massachusetts 01605, USA.
Peter M
PM
Ihnat
AbbVie Bioresearch Center, Worcester, Massachusetts 01605, USA.
Dana I
DI
Filoti
Center for Functional Nanomaterials, Brookhaven National Laboratory, Upton, New York 11973, USA.
Ming
M
Lu
Department of Mechanical Engineering, Virginia Tech, Blacksburg, Virginia 24061, USA.
Lei
L
Zuo
D002152:Calorimetry, Differential Scanning / Q000295:instrumentation / Q000379:methods; D056656:Lab-On-A-Chip Devices; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation / Q000379:methods