The influence of acute hypoxia on the functional and morphological state of the black scorpionfish red blood cells.
The investigation of the mechanisms of red blood cell steadiness to the oxygen lack in tolerant teleosts is of current scientific interest. Black scorpionfish, Scorpaena porcus L., is a widespread benthal species in the Black Sea and is highly resistant to hypoxic influence. The morphological state of black scorpionfish red blood cells under acute hypoxia was assessed using DNA-binding dye SYBR Green I and fluorescent microscopy. Changes in membrane potential of mitochondria and functional activity of cells were determined by rhodamine 123 (R123) and fluorescein diacetate (FDA) fluorescence. Oxygen deficiency leads to bidirectional changes in volume of erythrocytes and their nuclei. Between 0.57 and 1.76 mg О2l-1, both parameters increased on 3-12 and 7-21%, respectively. At 1.76-4.03, cells shrank on 1.5-6.0% and nucleus size decreased on 1.5-3%. Acute hypoxia induced a significant increase of R123 (12-60%) and FDA (30-184%) fluorescence. These reactions are caused by a probable decrease in erythrocyte membrane permeability.
Department of Animal Physiology and Biochemistry, Institute of Marine Biological Research, Russian Academy of Sciences, Leninsky Ave, 14, Moscow, Russia, 119991. andreevaal@gmail.com.
Aleksandra Y
AY
Andreyeva
Department of Animal Physiology and Biochemistry, Institute of Marine Biological Research, Russian Academy of Sciences, Leninsky Ave, 14, Moscow, Russia, 119991.
Aleksander A
AA
Soldatov
Department of Plankton, Institute of Marine Biological Research, Russian Academy of Sciences, Moscow, Russia.
Vladimir S
VS
Mukhanov
D000818:Animals; D015687:Cell Hypoxia; D002467:Cell Nucleus / Q000378:metabolism; D048430:Cell Shape; D004911:Erythrocyte Volume; D004912:Erythrocytes / Q000378:metabolism / Q000473:pathology*; D005434:Flow Cytometry; D005452:Fluoresceins / Q000378:metabolism; D005453:Fluorescence; D010473:Perciformes / Q000378:metabolism*; D020112:Rhodamine 123 / Q000378:metabolism
D005452:Fluoresceins; D020112:Rhodamine 123; C018506:diacetylfluorescein
Erythrocytes; Flow cytometry; Hypoxia; In vitro; Scorpaena porcus L.