Linking expression of aquaporin 3 to activation of JNK pathway.
Aquaporin 3 (AQP3) has been shown to be low in the amnion and chorion tissues of patients with oligohydramnios and thatS. miltiorrhiza,a Chinese herbal medicine, results in increased AQP3 in human amniotic epithelial cells (hAECs). Here, we provide evidence for the involvement of the JNK pathway in AQP3 regulation in isolated oligohydramnios tissuesin vitro,in hAECs derived from normal amniotic fluid and fluid from patients with isolated oligohydramnios. Phosphorylation of JNK was suppressed by pretreatment of cells with JNK-specific inhibitor (SP600125) and was up-regulated byS. miltiorrhiza;S. miltiorrhizacombined with SP600125 prevented SP600125-induced down-regulation of phospho-JNK both in normal amniotic fluid volume and in isolated oligohydramnios. In isolated oligohydramnios, AQP3 expression was signiﬁcantly suppressed by SP600125 in a concentration- and time-dependent mannner, while its expression was up-regulated byS. miltiorrhiza.S. miltiorrhizacombined with SP600125 inhibited the increased expression of AQP3 relative to theS. miltiorrhizatreated group. Together, the data suggest that c-jun N-terminal kinase (JNK) pathway unerlies the regulation of AQP3 byS. miltiorrhizaamnion and chorion tissues.
Department of Obstetrics and Gynecology, the Second Affiliated Hospital of Wenzhou Medical University, Wenzhou 325027, China.
Qi
Q
Shen
Department of Obstetrics and Gynecology, the Second Affiliated Hospital of Wenzhou Medical University, Wenzhou 325027, China.
Jingjing
J
Wang
Department of Obstetrics and Gynecology, The Second Affiliated Hospital of Wenzhou Medical University, Wenzhou 325027, China.
Qingfeng
Q
Zhou
Department of Obstetrics and Gynecology, The Second Affiliated Hospital of Wenzhou Medical University, Wenzhou 325027, China.
Zhaojun
Z
Shen
Department of Obstetrics and Gynecology, The Second Affiliated Hospital of Wenzhou Medical University, Wenzhou 325027, China.
Hui
H
Luo
Department of Obstetrics and Gynecology, The Second Affiliated Hospital of Wenzhou Medical University, Wenzhou 325027, China.
Xuejiao
X
Tao
Department of Obstetrics and Gynecology, the Second Affiliated Hospital of Wenzhou Medical University, Wenzhou 325027, China, zjwzzxq@yeah.net.
Xueqiong
X
Zhu
D000328:Adult; D000650:Amnion / Q000187:drug effects / Q000378:metabolism; D000653:Amniotic Fluid / Q000187:drug effects; D000873:Anthracenes / Q000008:administration & dosage; D051400:Aquaporin 3 / Q000378:metabolism*; D016022:Case-Control Studies; D002478:Cells, Cultured; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage; D004847:Epithelial Cells / Q000187:drug effects / Q000378:metabolism; D005260:Female; D006801:Humans; D020935:MAP Kinase Signaling System / Q000187:drug effects; D016104:Oligohydramnios / Q000188:drug therapy / Q000378:metabolism*; D011247:Pregnancy; D027561:Salvia miltiorrhiza; D055815:Young Adult
C496027:AQP3 protein, human; D000873:Anthracenes; D004365:Drugs, Chinese Herbal; D051400:Aquaporin 3; C432165:pyrazolanthrone