Distinct expression profiles of lncRNAs between early-onset preeclampsia and preterm controls.
Early-onset preeclampsia (EOPE), which is the most severe form of the syndrome, confers a high risk of neonatal morbidity and perinatal death. We aim to study the roles of long non-coding RNAs (lncRNAs) in the pathogenesis of early-onset preeclampsia (EOPE). Therefore, we examined the expression profiles of lncRNAs between early-onset preeclampsia and preterm controls using microarray analysis. Quantitative real-time PCR (qRT-PCR) was performed to verify the selected differentially expressed lncRNAs. In total, we identified 15,646 upregulated and 13,178 downregulated lncRNAs in the placenta of EOPE patients compared to the preterm controls. Gene ontology and pathway analysis revealed that compared to the preterm controls, many of the processes over-represented in the EOPE patients were related to cell migration and cell motility. A selection of the differentially expressed lncRNA transcripts was confirmed using qRT-PCR, particularly RP11-465L10.10, which is associated with the MMP9 gene. These data may offer a background/reference resource for future functional studies of lncRNAs related to EOPE.
Department of Obstetrics, Obstetrics and Gynecology Hospital Affiliated to Nanjing Medical University, China.
Wei
W
Long
Department of Obstetrics, Obstetrics and Gynecology Hospital Affiliated to Nanjing Medical University, China.
Can
C
Rui
Department of Obstetrics, Obstetrics and Gynecology Hospital Affiliated to Nanjing Medical University, China.
Xuejing
X
Song
Department of Obstetrics, Obstetrics and Gynecology Hospital Affiliated to Nanjing Medical University, China.
Xiaonan
X
Dai
Department of Obstetrics, Obstetrics and Gynecology Hospital Affiliated to Nanjing Medical University, China.
Xuan
X
Xue
Department of Obstetrics, Obstetrics and Gynecology Hospital Affiliated to Nanjing Medical University, China.
Yuanqing
Y
Lu
Department of Obstetrics, Obstetrics and Gynecology Hospital Affiliated to Nanjing Medical University, China.
Rong
R
Shen
Maternal and Child Health Medical Institute, Department of Plastic & Cosmetic Surgery, Obstetrics and Gynecology Hospital Affiliated to Nanjing Medical University, China.
Jun
J
Li
Maternal and Child Health Medical Institute, Department of Plastic & Cosmetic Surgery, Obstetrics and Gynecology Hospital Affiliated to Nanjing Medical University, China. Electronic address: dagumahao@163.com.
Jingyun
J
Li
Department of Obstetrics, Obstetrics and Gynecology Hospital Affiliated to Nanjing Medical University, China. Electronic address: dhj_njfy@126.com.
Hongjuan
H
Ding
D016022:Case-Control Studies; D015536:Down-Regulation; D005260:Female; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis; D011225:Pre-Eclampsia / Q000235:genetics* / Q000378:metabolism; D011247:Pregnancy; D062085:RNA, Long Noncoding / Q000235:genetics* / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D059467:Transcriptome; D015854:Up-Regulation
D062085:RNA, Long Noncoding
Early-onset preeclampsia; LncRNA; MMP9; Microarray; Real-time PCR