The histocompatibility research of hair follicle stem cells with bladder acellular matrix.
BACKGROUND
Hair follicle stem cells (HFSCs) were reported to have multidirectional differentiation ability and could be differentiated into melanocytes, keratin cells, smooth muscle cells, and neurons. However, the functionality of HFSCs in bladder tissue regeneration is unknown.


METHODS
This study was conducted to build HFSCs vs bladder acellular matrix (BAM) complexes (HFSCs-BAM complexes) in vitro and evaluated whether HFSCs have well biocompatibility with BAM. HFSCs were separated from SD rats. BAM scaffold was prepared from the submucosa of rabbit bladder tissue. Afterwards, HFSCs were inoculated on BAM.


RESULTS
HFSCs-BAM complexes grew rapidly through inverted microscope observation. Cell growth curve showed the proliferation was in stagnate phase at 7th and 8th day. Cytotoxicity assay showed the toxicity grading of BAM was 0 or 1. Scanning electron microscopy, HE staining, and masson staining showed that cells have germinated on the surface of scaffold.


CONCLUSIONS
The results provide evidence that HFSCs-BAM complexes have well biocompatibility and accumulate important experimental basis for clinical applying of tissue engineering bladder.
Urinary Center and Pediatric Center, The First Affiliated Hospital of Xinjiang Medical University Department of Urology, Renmin Hospital of Xinjiang Uygur Autonomous Region Urinary Center, The First Affiliated Hospital of Xinjiang Medical University, Urumqi City, Xinjiang, China.
Jia
J
Li
Wenguang
W
Wang
Jiuzhi
J
Li
Mulati
M
Rexiati
Henqing
H
An
Feng
F
Wang
Yujie
Y
Wang
D000818:Animals; D002454:Cell Differentiation; D018859:Hair Follicle / Q000166:cytology*; D006648:Histocompatibility; D051381:Rats; D013234:Stem Cells / Q000276:immunology*; D001743:Urinary Bladder