The microRNA-635 suppresses tumorigenesis in non-small cell lung cancer.
The microRNAs represent a class of noncoding RNAs with short length and play diverse roles in many biological processes. Despite tremendous effects have been devoted, the role of miR-635 in non-small cell lung cancer (NSCLC) remains elusive. Here we report a potential tumor suppressive function of miR-635 in NSCLC. By microRNA screening based on invasion assays, we identified series of functional miRNAs. The miR-635 was then identified as a potent inhibitor of cell invasion and differentially expressed in normal and cancerous tissues. We further confirmed that Ying Yang 1 (YY1) may be the direct target of miR-635 as miR-635 transfection can significantly decrease endogenous YY1 expression which can mimic the effect of siRNA-mediated YY1 knockdown. Meanwhile, both miR-635 transfection and YY1 silencing can attenuate NSCLC cell invasion. In addition, miR-635 transfection can also significantly inhibit the growth of xenograft tumors. Taken together, our results have suggested a novel tumor suppressive role for miR-635 in NSCLC.
Department of Respiratory Medicine, Jining NO.1 People's Hospital, Jining, 272011, China.
Yan
Y
Zhang
Department of Oncology‬, Jining NO.1 People's Hospital, Jining, 272011, China.
Zongwen
Z
Sun
Department of Health Care, Laiwu People's Hospital, Laiwu, 271100, China.
Yanqun
Y
Zhang
Department of Respiratory Medicine, Jining NO.1 People's Hospital, Jining, 272011, China.
Tian
T
Fu
Department of Clinical Laboratory, Jining NO.1 People's Hospital, Jining, 272011, China.
Cun
C
Liu
Department of Respiratory Medicine, Jining NO.1 People's Hospital, Jining, 272011, China.
Yongrui
Y
Liu
Department of Respiratory Medicine, Jining NO.1 People's Hospital, Jining, 272011, China. Electronic address: linyong_jph@163.com.
Yong
Y
Lin
D000368:Aged; D000818:Animals; D001483:Base Sequence; D063646:Carcinogenesis / Q000235:genetics* / Q000473:pathology; D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics* / Q000473:pathology; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000235:genetics; D018450:Disease Progression; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics* / Q000473:pathology; D008297:Male; D051379:Mice; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D008875:Middle Aged; D009361:Neoplasm Invasiveness; D050986:YY1 Transcription Factor / Q000235:genetics
C000614096:MIRN635 microRNA, human; D035683:MicroRNAs; D050986:YY1 Transcription Factor
Invasion; NSCLC; YY1; miR-635