The Arabidopsis Polycomb Repressive Complex 1 (PRC1) Components AtBMI1A, B, and C Impact Gene Networks throughout All Stages of Plant Development.
Polycomb Group regulation in Arabidopsis (Arabidopsis thaliana) is required to maintain cell differentiation and allow developmental phase transitions. This is achieved by the activity of three PcG repressive complex 2s (PRC2s) and the participation of a yet poorly defined PRC1. Previous results showed that apparent PRC1 components perform discrete roles during plant development, suggesting the existence of PRC1 variants; however, it is not clear in how many processes these components participate. We show that AtBMI1 proteins are required to promote all developmental phase transitions and to control cell proliferation during organ growth and development, expanding their proposed range of action. While AtBMI1 function during germination is closely linked to B3 domain transcription factors VAL1/2 possibly in combination with GT-box binding factors, other AtBMI1 regulatory networks require participation of different factor combinations. Conversely, EMF1 and LHP1 bind many H3K27me3 positive genes up-regulated in atbmi1a/b/c mutants; however, loss of their function affects expression of a different subset, suggesting that even if EMF1, LHP1, and AtBMI1 exist in a common PRC1 variant, their role in repression depends on the functional context.
Institute of Plant Biochemistry and Photosynthesis, 41092 Seville, Spain (W.M., A.G.-Z., M.C.).|Department of Computer Science and Artificial Intelligence, University of Seville, 41012 Seville, Spain (F.J.R.-C.); and.|Max Planck Institute for Plant Breeding Research, Department of Plant Developmental Biology, 50829 Cologne, Germany (F.T.).
Wiam
0000-0001-6606-7978
W
Merini
Institute of Plant Biochemistry and Photosynthesis, 41092 Seville, Spain (W.M., A.G.-Z., M.C.).|Department of Computer Science and Artificial Intelligence, University of Seville, 41012 Seville, Spain (F.J.R.-C.); and.|Max Planck Institute for Plant Breeding Research, Department of Plant Developmental Biology, 50829 Cologne, Germany (F.T.).
Francisco J
0000-0001-9834-030X
FJ
Romero-Campero
Institute of Plant Biochemistry and Photosynthesis, 41092 Seville, Spain (W.M., A.G.-Z., M.C.).|Department of Computer Science and Artificial Intelligence, University of Seville, 41012 Seville, Spain (F.J.R.-C.); and.|Max Planck Institute for Plant Breeding Research, Department of Plant Developmental Biology, 50829 Cologne, Germany (F.T.).
Angeles
0000-0003-4921-0212
A
Gomez-Zambrano
Institute of Plant Biochemistry and Photosynthesis, 41092 Seville, Spain (W.M., A.G.-Z., M.C.).|Department of Computer Science and Artificial Intelligence, University of Seville, 41012 Seville, Spain (F.J.R.-C.); and.|Max Planck Institute for Plant Breeding Research, Department of Plant Developmental Biology, 50829 Cologne, Germany (F.T.).
Yue
Y
Zhou
Institute of Plant Biochemistry and Photosynthesis, 41092 Seville, Spain (W.M., A.G.-Z., M.C.).|Department of Computer Science and Artificial Intelligence, University of Seville, 41012 Seville, Spain (F.J.R.-C.); and.|Max Planck Institute for Plant Breeding Research, Department of Plant Developmental Biology, 50829 Cologne, Germany (F.T.).
Franziska
0000-0002-4982-5949
F
Turck
Institute of Plant Biochemistry and Photosynthesis, 41092 Seville, Spain (W.M., A.G.-Z., M.C.); myriam.calonje@ibvf.csic.es.|Department of Computer Science and Artificial Intelligence, University of Seville, 41012 Seville, Spain (F.J.R.-C.); and myriam.calonje@ibvf.csic.es.|Max Planck Institute for Plant Breeding Research, Department of Plant Developmental Biology, 50829 Cologne, Germany (F.T.) myriam.calonje@ibvf.csic.es.
Myriam
0000-0001-6351-6774
M
Calonje
D017360:Arabidopsis / Q000235:genetics* / Q000254:growth & development*; D029681:Arabidopsis Proteins / Q000235:genetics* / Q000378:metabolism; D049109:Cell Proliferation / Q000235:genetics; D056625:Endosperm / Q000235:genetics / Q000254:growth & development; D018506:Gene Expression Regulation, Plant; D053263:Gene Regulatory Networks; D018745:Genome, Plant; D005964:Glucosyltransferases / Q000235:genetics / Q000378:metabolism; D008239:Lysine / Q000378:metabolism; D018519:Meristem / Q000235:genetics; D046912:Multiprotein Complexes; D009154:Mutation; D057445:Plant Dormancy / Q000235:genetics; D018517:Plant Roots / Q000235:genetics / Q000254:growth & development; D063150:Polycomb Repressive Complex 1 / Q000235:genetics*; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D002868:Chromosomal Proteins, Non-Histone
D029681:Arabidopsis Proteins; D005964:Glucosyltransferases; D008239:Lysine; D046912:Multiprotein Complexes; D063150:Polycomb Repressive Complex 1; D012097:Repressor Proteins; D014157:Transcription Factors; C000624995:BMI1A protein, Arabidopsis; C000624996:BMI1B protein, Arabidopsis; C000624997:BMI1C protein, Arabidopsis; C435921:EMF1 protein, Arabidopsis; C504531:HSI2 protein, Arabidopsis; C457769:PRC1 protein, Arabidopsis; C477788:At5g17690 protein, Arabidopsis; D002868:Chromosomal Proteins, Non-Histone