Crosslinked, cryostructured Lactobacillus reuteri monoliths for production of 3-hydroxypropionaldehyde, 3-hydroxypropionic acid and 1,3-propanediol from glycerol.
Crosslinked, cryostructured monoliths prepared from Lactobacillus reuteri cells were evaluated as potential immobilized whole-cell biocatalyst for conversion of glycerol, to potentially important chemicals for the biobased industry, i.e. 3-hydroxypropionaldehyde (3HPA), 3-hydroxypropionic acid (3HP) and 1,3-propanediol (1,3PDO). Glutaraldehyde, oxidized dextran and activated polyethyleneimine/modified polyvinyl alcohol (PEI/PVA) were evaluated as crosslinkers; the latter gave highly stable preparations with maintained viability and biocatalytic activity. Scanning electron microscopy of the PEI/PVA monoliths showed high density of crosslinked cells with wide channels allowing liquid flow through. Flux analysis of the propanediol-utilization pathway, incorporating glycerol/diol dehydratase, propionaldehyde dehydrogenase, 1,3PDO oxidoreductase, phosphotransacylase, and propionate kinase, for conversion of glycerol to the three chemicals showed that the maximum specific reaction rates were -562.6, 281.4, 62.4 and 50.5mg/gCDWh for glycerol consumption, and 3HPA (extracellular), 3HP and 1,3PDO production, respectively. Under optimal conditions using monolith operated as continuous plug flow reactor, 19.7g/L 3HPA was produced as complex with carbohydrazide at a rate of 9.1g/Lh and a yield of 77mol%. Using fed-batch operation, 1,3PDO and 3HP were co-produced in equimolar amounts with a yield of 91mol%. The monoliths embedded in plastic carriers showed high mechanical stability under different modes in a miniaturized plug flow reactor.
Biotechnology, Department of Chemistry, Center for Chemistry and Chemical Engineering, Lund University, Box 124, SE-221 00 Lund, Sweden.
Oksana
O
Zaushitsyna
Biotechnology, Department of Chemistry, Center for Chemistry and Chemical Engineering, Lund University, Box 124, SE-221 00 Lund, Sweden; Department of Microbiology and Immunology, Faculty of Pharmacy, Beni-Suef University, 62511 Beni-Suef, Egypt.
Tarek
T
Dishisha
Biotechnology, Department of Chemistry, Center for Chemistry and Chemical Engineering, Lund University, Box 124, SE-221 00 Lund, Sweden. Electronic address: Rajni.Hatti-Kaul@biotek.lu.se.
Rajni
R
Hatti-Kaul
Biotechnology, Department of Chemistry, Center for Chemistry and Chemical Engineering, Lund University, Box 124, SE-221 00 Lund, Sweden.
Bo
B
Mattiasson
D018914:Cells, Immobilized / Q000166:cytology / Q000378:metabolism*; D059985:Cryogels / Q000737:chemistry; D005985:Glyceraldehyde / Q000031:analogs & derivatives* / Q000032:analysis / Q000378:metabolism; D005990:Glycerol / Q000378:metabolism*; D019344:Lactic Acid / Q000031:analogs & derivatives* / Q000032:analysis / Q000378:metabolism; D052200:Limosilactobacillus reuteri / Q000166:cytology / Q000378:metabolism*; D011407:Propane / Q000032:analysis / Q000378:metabolism*; D011409:Propylene Glycols / Q000032:analysis / Q000378:metabolism*
D059985:Cryogels; D011409:Propylene Glycols; C047158:3-hydroxypropionaldehyde; D019344:Lactic Acid; D005985:Glyceraldehyde; C041787:1,3-propanediol; C031601:hydracrylic acid; D005990:Glycerol; D011407:Propane
Cell immobilization; Crosslinking; Cryogel monolith; Flux analysis; Glycerol; Lactobacillus reuteri