High expression of muscarinic acetylcholine receptor 3 predicts poor prognosis in patients with pancreatic ductal adenocarcinoma.
OBJECTIVE
Recent studies showed that muscarinic acetylcholine receptor 3 (M3), as a muscarinic acetylcholine receptor family member that plays an important role in normal physiological function, is engaged in cancer progression. However, the role of M3 in pancreatic ductal adenocarcinoma (PDAC) is not known. The aim of this study is to investigate the expression and prognostic value of M3 in patients with PDAC.


METHODS
The localization and expression of M3 in PDAC were examined by immunohistochemistry. VAChT was employed to detect parasympathetic nerve fibers in the corresponding M3 PDAC tissues. The correlation between M3 expression and patients' survival was assessed by Kaplan-Meier analysis.


RESULTS
M3 was discovered predominantly localized in the cell cytoplasm and expressed in all specimens of PDAC patients. Significant correlation was noted between increased M3 intensity and high grade of PDAC (P<0.01), more lymph node metastasis (P<0.01) as well as shorter patient overall survival (P<0.01). Morphologically, cells with high M3 expression were more frequently located at the invasive tumor front/tumor budding cells, metastatic lymph nodes and parasympathetic nerve fibers.


CONCLUSIONS
High expression of M3 is a prognostic marker for PDAC.
Department of General Surgery, Peking University Third Hospital.
Lingfu
L
Zhang
Department of General Surgery, Peking University Third Hospital.
Dianrong
D
Xiu
Key Laboratory of Carcinogenesis and Translational Research, Ministry of Education, State Key Laboratory of Natural and Biomimetic Drugs; Laboratory of Molecular Cell Biology and Tumor Biology, Department of Anatomy, Histology and Embryology.
Jun
J
Zhan
Laboratory of Molecular Cell Biology and Tumor Biology, Department of Anatomy, Histology and Embryology.
Xiaokun
X
He
Department of Pathology, Peking University Health Science Center; Department of Pathology, Peking University Third Hospital, Beijing, People's Republic of China.
Limei
L
Guo
Department of General Surgery, Peking University Third Hospital.
Jilian
J
Wang
Department of General Surgery, Peking University Third Hospital.
Ming
M
Tao
Department of General Surgery, Peking University Third Hospital.
Wei
W
Fu
Key Laboratory of Carcinogenesis and Translational Research, Ministry of Education, State Key Laboratory of Natural and Biomimetic Drugs; Laboratory of Molecular Cell Biology and Tumor Biology, Department of Anatomy, Histology and Embryology.
Hongquan
H
Zhang
M3; PDAC; muscarinic acetylcholine receptor 3; parasympathetic nerve fiber; prognosis; tumor budding