Ameliorative effect of Trametes orientalis polysaccharide against immunosuppression and oxidative stress in cyclophosphamide-treated mice.
The present study evaluated the ameliorative effects of Trametes orientalis polysaccharide (TOP-2) against cyclophosphamide (CP) induced toxicity in mice. Intraperitoneal administration of TOP-2 not only effectively increased the thymus, spleen, heart, liver, and kidney indices, but also significantly enhanced the phagocytic activities of macrophages and splenocyte proliferation, dose-dependently. The lowered nitric oxide level of macrophages after CP treatment was elevated by TOP-2 administration. Impaired splenic natural killer cells and cytotoxic T lymphocytes activities were remarkably enhanced by TOP-2. Furthermore, the levels of interleukin-2, interferon-γ, Immunoglobulin A, immunoglobulin G, and immunoglobulin M were notably reduced by CP, while TOP-2 abolished these effects. TOP-2 could also effectively increase the total antioxidant capacity, superoxidase dismutase, catalase and glutathione peroxidase activities, and inhibit the increase in malondialdehyde level. These results indicate that TOP-2 may be of therapeutic value in ameliorating the immunosuppression and oxidative stress caused by CP treatment, through its immunomodulatory and antioxidant potential.
Key Laboratory of Coal Processing and Efficient Utilization, Ministry of Education, China University of Mining & Technology, Xuzhou 221116, China; Jiangsu Key Laboratory of Food Resource Development and Quality Safe, Xuzhou Institute of Technology, Xuzhou 221000, China.
Yi
Y
Zheng
Key Laboratory of Coal Processing and Efficient Utilization, Ministry of Education, China University of Mining & Technology, Xuzhou 221116, China. Electronic address: zong_zhimin@163.com.
Zhi-Min
ZM
Zong
Key Laboratory of Coal Processing and Efficient Utilization, Ministry of Education, China University of Mining & Technology, Xuzhou 221116, China; Jiangsu Key Laboratory of Food Resource Development and Quality Safe, Xuzhou Institute of Technology, Xuzhou 221000, China.
Shang-Long
SL
Chen
Jiangsu Key Laboratory of Food Resource Development and Quality Safe, Xuzhou Institute of Technology, Xuzhou 221000, China.
An-Hui
AH
Chen
Key Laboratory of Coal Processing and Efficient Utilization, Ministry of Education, China University of Mining & Technology, Xuzhou 221116, China.
Xian-Yong
XY
Wei
D000818:Animals; D000893:Anti-Inflammatory Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D000975:Antioxidants / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D002374:Catalase / Q000276:immunology / Q000378:metabolism; D003520:Cyclophosphamide / Q000009:adverse effects / Q000037:antagonists & inhibitors*; D005260:Female; D062610:Fungal Polysaccharides / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D005979:Glutathione Peroxidase / Q000276:immunology / Q000378:metabolism; D006321:Heart / Q000187:drug effects; D007165:Immunosuppression Therapy; D007166:Immunosuppressive Agents / Q000009:adverse effects / Q000037:antagonists & inhibitors*; D007668:Kidney / Q000187:drug effects / Q000276:immunology; D007694:Killer Cells, Natural / Q000187:drug effects / Q000276:immunology; D008099:Liver / Q000187:drug effects / Q000276:immunology; D008264:Macrophages / Q000187:drug effects / Q000276:immunology; D008297:Male; D008315:Malondialdehyde / Q000276:immunology / Q000378:metabolism; D051379:Mice; D018384:Oxidative Stress / Q000187:drug effects; D010587:Phagocytosis / Q000187:drug effects; D013154:Spleen / Q000187:drug effects / Q000276:immunology; D013482:Superoxide Dismutase / Q000276:immunology / Q000378:metabolism; D013602:T-Lymphocytes, Cytotoxic / Q000187:drug effects / Q000276:immunology; D013950:Thymus Gland / Q000187:drug effects / Q000276:immunology; D055454:Trametes / Q000737:chemistry*
D000893:Anti-Inflammatory Agents; D000975:Antioxidants; D062610:Fungal Polysaccharides; D007166:Immunosuppressive Agents; D008315:Malondialdehyde; D003520:Cyclophosphamide; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase
Antioxidant; Immunomodulatory; Trametes orientalis polysaccharide