Ethanol extract of Lophatheri Herba exhibits anti-cancer activity in human cancer cells by suppression of metastatic and angiogenic potential.
Lophatheri Herba (LH), dried leaf of Lophatherum gracile Brongn, has long been used to reduce thirst and treat fever and inflammation in Chinese medicine. Recent studies have shown that LH has anti-viral, anti-bacterial, anti-cancer, anti-oxidant, diuretic, and hyperglycemic properties. However, the effects of an ethanol extract of L. herba (ELH), at non-cytotoxic doses, on the metastatic and angiogenic abilities of malignant tumor cells have not been reported. We found that ELH significantly suppressed p38, JNK, and NF-κB activation and proteolytic activities under phorbol 12-myristate 13-acetate (PMA) stimulation, thus leading to a decrease in metastatic potential, including migration and invasion. In addition, ELH suppressed tumor-induced angiogenesis, including migration and tube formation in human umbilical vein endothelial cells (HUVECs) and microvessel sprouting from aortic rings via decreasing the pro-angiogenic factors in tumors. Interestingly, in ovo xenografts ELH-treated HT1080 cells did not increase in volume and eventually disappeared, owing to a lack of angiogenesis. Daily oral administration of ELH at 50 and 100 mg/kg markedly inhibited metastatic colonization of B16F10 cells in the lungs of C57BL/6J mice and caused no apparent side effects. These data collectively indicate that ELH is safe and may be useful for managing metastasis and growth of malignant cancers.
Korean Medicine (KM) Application Center, Korea Institute of Oriental Medicine (KIOM), 70 Chumdan-ro, Dong-gu, Daegu 701-300, Republic of Korea.
Aeyung
A
Kim
Korean Medicine (KM) Application Center, Korea Institute of Oriental Medicine (KIOM), 70 Chumdan-ro, Dong-gu, Daegu 701-300, Republic of Korea.
Minju
M
Im
Korean Medicine (KM) Application Center, Korea Institute of Oriental Medicine (KIOM), 70 Chumdan-ro, Dong-gu, Daegu 701-300, Republic of Korea.
Min Jung
MJ
Gu
Korean Medicine (KM) Application Center, Korea Institute of Oriental Medicine (KIOM), 70 Chumdan-ro, Dong-gu, Daegu 701-300, Republic of Korea.
Jin Yeul
JY
Ma
D020533:Angiogenesis Inhibitors / Q000737:chemistry / Q000494:pharmacology*; D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000494:pharmacology*; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D002642:Chick Embryo; D000431:Ethanol / Q000737:chemistry; D005260:Female; D006801:Humans; D008297:Male; D008810:Mice, Inbred C57BL; D008813:Mice, Inbred ICR; D009362:Neoplasm Metastasis; D009369:Neoplasms / Q000098:blood supply / Q000188:drug therapy / Q000473:pathology; D009389:Neovascularization, Pathologic / Q000473:pathology / Q000517:prevention & control; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D006109:Poaceae / Q000737:chemistry*; D017207:Rats, Sprague-Dawley
D020533:Angiogenesis Inhibitors; D000972:Antineoplastic Agents, Phytogenic; D010936:Plant Extracts; D000431:Ethanol