SAPOVIRUSES IN CHILDREN WITH ACUTE GASTROENTERITIS FROM MANAUS , AMAZON REGION, BRAZIL, 2010-2011.
Sapoviruses (SaVs) are responsible for acute gastroenteritis in humans, especially children and the elderly. In Brazil, data on SaVs infections are very limited, especially in Northern Brazil. Here, we investigated the occurrence of SaVs in samples from hospitalized children under ten years old that presented acute gastroenteritis. Positive samples were genotyped and phylogenetic analysis was performed using prototype strains sequences obtained from GenBank database. In total, 156 fecal samples were screened by RT-PCR for SaVs. A positivity rate of 3.8% (6/156) was found in children under three years of age. Four genotypes were detected: GI.I, GI.2 and GII.2?-GII.4?/GII.4, suggesting a possible inter-genotypes recombination. Most infections (83.3%) occurred between August and September. The positivity was similar to that found in other countries and genotyping demonstrated the presence of distinct genotypes. To our knowledge, this is the first study reporting the circulation of SaVs in Manaus, state of Amazonas, Amazon region, Brazil.
Evandro Chagas Institute, Postgraduate Program in Virology. Ananindeua, Pará, Brazil. E-mails: tammykathlyn@gmail.com; julianamhernandez@gmail.com.
Tammy Kathlyn Amaral
TK
Reymão
Evandro Chagas Institute, Postgraduate Program in Virology. Ananindeua, Pará, Brazil. E-mails: tammykathlyn@gmail.com; julianamhernandez@gmail.com.
Juliana das Merces
JD
Hernandez
University of the State of Pará, Postgraduate Program in Parasitic, Biology of the Amazon. Belém, Pará, Brazil. E-mail: costasamya@hotmail.com.
Samya Thalita Picanço da
ST
Costa
Federal University of Pará, Tropical Medicine Center, Postgraduate Program in Tropical Diseases. Belém, Pará, Brazil. E-mail: maisaufpa@gmail.com.
Maísa Silva de
MS
Sousa
Evandro Chagas Institute, Brazilian Ministry of Health, Virology Section. Ananindeua, Pará, Brazil. E-mails: darleiseoliveira@iec.pa.gov.br; lucianasilva@iec.pa.gov.br; renatobandeira@iec.pa.gov.br; ianlima@iec.pa.gov.br; luanasoares@iec.pa.gov.br; joanamascarenhas@iec.pa.gov.br.
Darleise de Souza
DS
Oliveira
Evandro Chagas Institute, Brazilian Ministry of Health, Virology Section. Ananindeua, Pará, Brazil. E-mails: darleiseoliveira@iec.pa.gov.br; lucianasilva@iec.pa.gov.br; renatobandeira@iec.pa.gov.br; ianlima@iec.pa.gov.br; luanasoares@iec.pa.gov.br; joanamascarenhas@iec.pa.gov.br.
Luciana Damascena da
LD
Silva
Evandro Chagas Institute, Brazilian Ministry of Health, Virology Section. Ananindeua, Pará, Brazil. E-mails: darleiseoliveira@iec.pa.gov.br; lucianasilva@iec.pa.gov.br; renatobandeira@iec.pa.gov.br; ianlima@iec.pa.gov.br; luanasoares@iec.pa.gov.br; joanamascarenhas@iec.pa.gov.br.
Renato da Silva
RD
Bandeira
Evandro Chagas Institute, Brazilian Ministry of Health, Virology Section. Ananindeua, Pará, Brazil. E-mails: darleiseoliveira@iec.pa.gov.br; lucianasilva@iec.pa.gov.br; renatobandeira@iec.pa.gov.br; ianlima@iec.pa.gov.br; luanasoares@iec.pa.gov.br; joanamascarenhas@iec.pa.gov.br.
Ian Carlos Gomes de
IC
Lima
Evandro Chagas Institute, Brazilian Ministry of Health, Virology Section. Ananindeua, Pará, Brazil. E-mails: darleiseoliveira@iec.pa.gov.br; lucianasilva@iec.pa.gov.br; renatobandeira@iec.pa.gov.br; ianlima@iec.pa.gov.br; luanasoares@iec.pa.gov.br; joanamascarenhas@iec.pa.gov.br.
Luana da Silva
LD
Soares
Evandro Chagas Institute, Brazilian Ministry of Health, Virology Section. Ananindeua, Pará, Brazil. E-mails: darleiseoliveira@iec.pa.gov.br; lucianasilva@iec.pa.gov.br; renatobandeira@iec.pa.gov.br; ianlima@iec.pa.gov.br; luanasoares@iec.pa.gov.br; joanamascarenhas@iec.pa.gov.br.
Joana Darc Pereira
JD
Mascarenhas
Evandro Chagas Institute, Brazilian Ministry of Health, Virology Section. Ananindeua, Pará, Brazil. E-mails: darleiseoliveira@iec.pa.gov.br; lucianasilva@iec.pa.gov.br; renatobandeira@iec.pa.gov.br; ianlima@iec.pa.gov.br; luanasoares@iec.pa.gov.br; joanamascarenhas@iec.pa.gov.br.
Yvone Benchimol
YB
Gabbay
D000208:Acute Disease; D001938:Brazil / Q000453:epidemiology; D017250:Caliciviridae Infections / Q000175:diagnosis / Q000453:epidemiology*; D002648:Child; D002675:Child, Preschool; D005243:Feces / Q000821:virology; D005759:Gastroenteritis / Q000175:diagnosis / Q000453:epidemiology* / Q000821:virology; D005838:Genotype; D006801:Humans; D010802:Phylogeny; D020133:Reverse Transcriptase Polymerase Chain Reaction; D029341:Sapovirus / Q000235:genetics* / Q000302:isolation & purification