Development and evaluation of an experimental protocol for 3-D visualization and characterization of the structure of bacterial biofilms in porous media using laboratory X-ray tomography.
The development of a reliable model allowing accurate predictions of biofilm growth in porous media relies on a good knowledge of the temporal evolution of biofilm structure within the porous network. Since little is known about the real 3-D structure of biofilms in porous media, this work was aimed at developing a new experimental protocol to visualize the 3-D microstructure of the inside of a porous medium using laboratory X-ray microtomography. A reliable and reproducible methodology is proposed for (1) growing a biofilm inside a porous medium, and (2) X-ray tomography-based characterization of the temporal development of the biofilm at the inlet of the biofilter. The statistical analysis proposed here also validates the results presented in the literature based on a biofilm structure single measurement.
a Laboratoire 3SR , Université Grenoble-Alpes/CNRS/G-INP, UMR 5521 , Grenoble , France.|b Division of Microbiology, Department of Biology, Faculty of Science , University of Zagreb , Zagreb , Croatia.|c Laboratoire LEGI , Université Grenoble-Alpes/CNRS/G-INP, UMR 5519 , Grenoble , France.
Tomislav
T
Ivankovic
a Laboratoire 3SR , Université Grenoble-Alpes/CNRS/G-INP, UMR 5521 , Grenoble , France.
Sabine
S
Rolland du Roscoat
a Laboratoire 3SR , Université Grenoble-Alpes/CNRS/G-INP, UMR 5521 , Grenoble , France.
Christian
C
Geindreau
c Laboratoire LEGI , Université Grenoble-Alpes/CNRS/G-INP, UMR 5519 , Grenoble , France.
Philipe
P
Séchet
c Laboratoire LEGI , Université Grenoble-Alpes/CNRS/G-INP, UMR 5519 , Grenoble , France.
Zhujun
Z
Huang
d Laboratoire LTHE Université Grenoble-Alpes/CNRS/G-INP/IRD, UMR 5564 , Grenoble , France.
Jean M F
JM
Martins
D018441:Biofilms / Q000254:growth & development*; D003287:Contrast Media / Q000737:chemistry; D021621:Imaging, Three-Dimensional / Q000379:methods*; D007753:Laboratories; D008962:Models, Theoretical; D009281:Naphthalenes / Q000737:chemistry; D016062:Porosity; D016958:Pseudomonas putida / Q000502:physiology*; D013499:Surface Properties; D055114:X-Ray Microtomography / Q000379:methods*
D003287:Contrast Media; D009281:Naphthalenes; C031337:1-chloronaphthalene
Biofilter; Pseudomonas putida; X-ray tomography; biofilm