Birth after human chorionic gonadotropin-primed oocytein vitromaturation and fertilization with testicular sperm in a normo-ovulatory patient.
In this report, we present a case ofin vitromaturation (IVM) with surgical retrieved testicular sperm in a normo-ovulatory female. Human chorionic gonadotropin-primed IVM, testicular biopsy for sperm retrieval and intracytoplasmic sperm injection with fresh sperm were performed. Fourteen cumulus-oocyte complexes were obtained in germinal vesicle or metaphase I stage, eight oocytes reached metaphase II, seven presumptive zygotes were obtained, and three cleavage stages embryos in day 2 were transferred producing a singleton pregnancy. A single healthy newborn was obtained. Our results suggest that IVM may be an alternative forin vitrofertilization in normo-ovulatory women even if surgical retrieval of sperm is needed. Further research is required to depict contributing factors to the success of IVM in indications different from polycystic ovaries syndrome and the role of male gamete.
Institute of Sciences in Human Reproduction Vida, Leon, Mexico.
Claudia
C
González-Ortega
School of Medicine, Medical Sciences and Nutrition, University of Aberdeen, Aberdeen, UK.
Raul Eduardo
RE
Piña-Aguilar
Institute of Sciences in Human Reproduction Vida, Leon, Mexico.
Patricia
P
Cancino-Villareal
Institute of Sciences in Human Reproduction Vida, Leon, Mexico.
Antonio Martin
AM
Gutiérrez-Gutiérrez
Azoospermia; germinal vesicle; in vitro maturation; intracytoplasmic sperm injection; mild approach; surgical retrieval of sperm