Cytotoxicity and oxidative stress responses of silica-coated iron oxide nanoparticles in CHSE-214 cells.
The present study aimed at investigating cytotoxicity and oxidative stress induced by silica-coated iron oxide nanoparticles functionalized with dithiocarbamate (Fe3O4NPs) in Chinook salmon cells (CHSE-214) derived from Oncorhynchus tshawytscha embryos. A significant reduction in cell viability was evident in response to Fe3O4NPs as revealed by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay after 24 h of exposure. Out of the tested concentrations (10, 20, and 30 μg/ml), the highest concentration has shown significant decrease in the viability of cells after 24 h of exposure. Alterations in the morphology of CHSE-214 cells was also evident at 10 μg/ml concentration of Fe3O4NPs after 24 h. Fe3O4NPs elicited a significant dose-dependent reduction in total glutathione content (TGSH), catalase (CAT), glutathione reductase (GR) with a concomitant increase in lipid peroxidation (LPO), and protein carbonyl (PC) at highest concentration (30 μg/ml) after 24 h of exposure. In conclusion, our data demonstrated that Fe3O4NPs have potential to induce cytotoxicity in CHSE-214 cells, which is likely to be mediated through reactive oxygen species generation and oxidative stress.
CESAM-Centre for Environmental and Marine Studies, Department of Chemistry, University of Aveiro, 3810-193, Aveiro, Portugal. koigooras@ua.pt.
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Srikanth
CESAM-Centre for Environmental and Marine Studies, Department of Chemistry, University of Aveiro, 3810-193, Aveiro, Portugal.|Department of Chemistry, CICECO and CESAM, Aveiro Institute of Nanotechnology, University of Aveiro, 3810-193, Aveiro, Portugal.
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Trindade
CESAM-Centre for Environmental and Marine Studies, Department of Chemistry, University of Aveiro, 3810-193, Aveiro, Portugal.
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Duarte
CESAM-Centre for Environmental and Marine Studies, Department of Chemistry, University of Aveiro, 3810-193, Aveiro, Portugal.
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Pereira
D000818:Animals; D002374:Catalase / Q000378:metabolism; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D005290:Ferric Compounds / Q000633:toxicity*; D005978:Glutathione / Q000378:metabolism; D005980:Glutathione Reductase / Q000378:metabolism; D015227:Lipid Peroxidation; D053758:Nanoparticles / Q000633:toxicity*; D018384:Oxidative Stress; D017382:Reactive Oxygen Species / Q000378:metabolism; D012474:Salmon / Q000378:metabolism; D012822:Silicon Dioxide / Q000633:toxicity*; D013778:Tetrazolium Salts; D013844:Thiazoles
D005290:Ferric Compounds; D017382:Reactive Oxygen Species; D013778:Tetrazolium Salts; D013844:Thiazoles; C000499:ferric oxide; D012822:Silicon Dioxide; D002374:Catalase; D005980:Glutathione Reductase; C022616:thiazolyl blue; D005978:Glutathione
Chinook salmon; Cytotoxicity; Iron oxide nanoparticles; Lipid peroxidation; Oxidative stress; Protein carbonyl