RANK Expression and Osteoclastogenesis in Human Monocytes in Peripheral Blood from Rheumatoid Arthritis Patients.
Rheumatoid arthritis (RA) appears as inflammation of synovial tissue and joint destruction. Receptor activator of NF-κB (RANK) is a member of the TNF receptor superfamily and a receptor for the RANK ligand (RANKL). In this study, we examined the expression of RANKhighand CCR6 on CD14+monocytes from patients with RA and healthy volunteers. Peripheral blood samples were obtained from both the RA patients and the healthy volunteers. Osteoclastogenesis from monocytes was induced by RANKL and M-CSFin vitro. To study the expression of RANKhighand CCR6 on CD14+monocytes, two-color flow cytometry was performed. Levels of expression of RANK on monocytes were significantly correlated with the level of osteoclastogenesis in the healthy volunteers. The expression of RANKhighon CD14+monocyte in RA patients without treatment was elevated and that in those receiving treatment was decreased. In addition, the high-level expression of RANK on CD14+monocytes was correlated with the high-level expression of CCR6 in healthy volunteers. Monocytes expressing both RANK and CCR6 differentiate into osteoclasts. The expression of CD14+RANKhighin untreated RA patients was elevated. RANK and CCR6 expressed on monocytes may be novel targets for the regulation of bone resorption in RA and osteoporosis.
Institute of Rheumatology, Tokyo Women's Medical University, 10-22 Kawada-cho, Shinjuku-ku, Tokyo 162-0054, Japan.
Yuki
0000-0001-6269-0703
Y
Nanke
Institute of Rheumatology, Tokyo Women's Medical University, 10-22 Kawada-cho, Shinjuku-ku, Tokyo 162-0054, Japan.
Tsuyoshi
T
Kobashigawa
Institute of Rheumatology, Tokyo Women's Medical University, 10-22 Kawada-cho, Shinjuku-ku, Tokyo 162-0054, Japan.
Toru
0000-0003-1393-8615
T
Yago
Institute of Rheumatology, Tokyo Women's Medical University, 10-22 Kawada-cho, Shinjuku-ku, Tokyo 162-0054, Japan.
Manabu
0000-0001-9442-6149
M
Kawamoto
Institute of Rheumatology, Tokyo Women's Medical University, 10-22 Kawada-cho, Shinjuku-ku, Tokyo 162-0054, Japan.
Hisashi
0000-0001-8453-6731
H
Yamanaka
Institute of Rheumatology, Tokyo Women's Medical University, 10-22 Kawada-cho, Shinjuku-ku, Tokyo 162-0054, Japan.
Shigeru
0000-0002-8922-0282
S
Kotake
D000328:Adult; D000368:Aged; D001172:Arthritis, Rheumatoid / Q000097:blood* / Q000378:metabolism*; D016022:Case-Control Studies; D002452:Cell Count; D005453:Fluorescence; D006801:Humans; D018950:Lipopolysaccharide Receptors / Q000378:metabolism; D008875:Middle Aged; D009000:Monocytes / Q000378:metabolism*; D010010:Osteoclasts / Q000378:metabolism*; D010012:Osteogenesis; D053246:Receptor Activator of Nuclear Factor-kappa B / Q000378:metabolism*; D054399:Receptors, CCR6 / Q000378:metabolism
C106311:CCR6 protein, human; D018950:Lipopolysaccharide Receptors; D053246:Receptor Activator of Nuclear Factor-kappa B; D054399:Receptors, CCR6; C505686:TNFRSF11A protein, human