The effects of hydration media on the characteristics of non-ionic surfactant vesicles (NISV) prepared by microfluidics.
Non-ionic surfactant vesicles (NISV) are colloidal particles that provide a useful delivery system for drugs and vaccines. One of the methods that is used for NISV preparation is microfluidics in which the lipid components dissolved in organic phase are mixed with an aqueous medium to prepare the particles through self-assembly of the lipids. In this work, we examined the effect of using different types of aqueous media on the characteristics of the NISV prepared by microfluidics. Five aqueous media were tested: phosphate buffered saline, HEPES buffer, Tris buffer, normal saline and distilled water. The resulting particles were tested for their physical characteristics and cytotoxicity. The aqueous media were found to have significant effects on the physical characteristics of the particles, as well as their overall stability under different conditions and their cytotoxicity to different human cell lines. Careful consideration should be taken when choosing the aqueous media for preparing NISV through microfluidics. This is an important factor that will also have implications with respect to the entrapped material, but which in addition may help to design vesicles for different uses based on changing the preparation medium.
Strathclyde Institute of Pharmacy and Biomedical Sciences, University of Strathclyde, 161 Cathedral Street, G4 0RE Glasgow, United Kingdom; Faculty of Pharmacy, Yarmouk University, Irbid, Jordan. Electronic address: mohmad-ali-radi-obid@strath.ac.uk.
Mohammad A
MA
Obeid
Strathclyde Institute of Pharmacy and Biomedical Sciences, University of Strathclyde, 161 Cathedral Street, G4 0RE Glasgow, United Kingdom.
Ibrahim
I
Khadra
Strathclyde Institute of Pharmacy and Biomedical Sciences, University of Strathclyde, 161 Cathedral Street, G4 0RE Glasgow, United Kingdom.
Alexander B
AB
Mullen
Strathclyde Institute of Pharmacy and Biomedical Sciences, University of Strathclyde, 161 Cathedral Street, G4 0RE Glasgow, United Kingdom.
Rothwelle J
RJ
Tate
Strathclyde Institute of Pharmacy and Biomedical Sciences, University of Strathclyde, 161 Cathedral Street, G4 0RE Glasgow, United Kingdom.
Valerie A
VA
Ferro
D002460:Cell Line; D002626:Chemistry, Pharmaceutical / Q000379:methods; D016503:Drug Delivery Systems; D004355:Drug Stability; D006801:Humans; D008055:Lipids / Q000737:chemistry*; D044085:Microfluidics / Q000379:methods*; D013501:Surface-Active Agents / Q000737:chemistry*; D018675:Toxicity Tests
D008055:Lipids; D013501:Surface-Active Agents
Drug delivery; Hydration media; Microfluidics; Non-ionic surfactant vesicles