A Major QTL, Which Is Co-located withcly1, and Two Minor QTLs Are Associated with Glume Opening Angle in Barley (Hordeum vulgareL.).
Cleistogamous and chasmogamous are two opposing phenomena for flowering in barley. Cleistogamy limits the rate of outcrossing, and increases the cost of producing hybrid barley seeds. Selecting chasmogamous lines with a large glume opening angle (GOA) is essential for the utilization of barley heterosis. In the current study, 247 DH lines derived from a cross between Yangnongpi7 and Yang0187 were used to identify and validate quantitative trait loci (QTLs) associated with the GOA in different environments using SSR markers. Three QTLs associated with barley GOA were mapped on chromosomes 2H and 7H. The major QTLQGOA-2H-2was mapped on chromosome 2H with the flanking markers of KDH and GBM1498, explaining 63.92% of the phenotypic variation. The marker KDH was developed from the geneCly1, which was the candidate gene forQGOA-2H-2. This new marker can be used to identify barley chasmogamous lines with a large GOA. The two minor QTLs were validated at all three locations across two seasons after removing DH lines carrying the candidate geneCly1ofQGOA-2H-2.
Jiangsu Key Laboratory of Crop Genetics and Physiology, Yangzhou UniversityYangzhou, China; Co-Innovation Center for Modern Production Technology of Grain Crops, Yangzhou UniversityYangzhou, China; Key Laboratory of Plant Functional Genomics of the Ministry of Education, Yangzhou UniversityYangzhou, China; Institute of Barley Research, Yangzhou UniversityYangzhou, China.
XinZhong
X
Zhang
Jiangsu Key Laboratory of Crop Genetics and Physiology, Yangzhou UniversityYangzhou, China; Co-Innovation Center for Modern Production Technology of Grain Crops, Yangzhou UniversityYangzhou, China; Key Laboratory of Plant Functional Genomics of the Ministry of Education, Yangzhou UniversityYangzhou, China; Institute of Barley Research, Yangzhou UniversityYangzhou, China.
BaoJian
B
Guo
Jiangsu Key Laboratory of Crop Genetics and Physiology, Yangzhou UniversityYangzhou, China; Co-Innovation Center for Modern Production Technology of Grain Crops, Yangzhou UniversityYangzhou, China; Key Laboratory of Plant Functional Genomics of the Ministry of Education, Yangzhou UniversityYangzhou, China; Institute of Barley Research, Yangzhou UniversityYangzhou, China.
GuoFang
G
Lan
Jiangsu Key Laboratory of Crop Genetics and Physiology, Yangzhou UniversityYangzhou, China; Co-Innovation Center for Modern Production Technology of Grain Crops, Yangzhou UniversityYangzhou, China; Key Laboratory of Plant Functional Genomics of the Ministry of Education, Yangzhou UniversityYangzhou, China; Institute of Barley Research, Yangzhou UniversityYangzhou, China.
HongTao
H
Li
Jiangsu Key Laboratory of Crop Genetics and Physiology, Yangzhou UniversityYangzhou, China; Co-Innovation Center for Modern Production Technology of Grain Crops, Yangzhou UniversityYangzhou, China; Key Laboratory of Plant Functional Genomics of the Ministry of Education, Yangzhou UniversityYangzhou, China; Institute of Barley Research, Yangzhou UniversityYangzhou, China.
Shen
S
Lin
Jiangsu Key Laboratory of Crop Genetics and Physiology, Yangzhou UniversityYangzhou, China; Co-Innovation Center for Modern Production Technology of Grain Crops, Yangzhou UniversityYangzhou, China; Key Laboratory of Plant Functional Genomics of the Ministry of Education, Yangzhou UniversityYangzhou, China; Institute of Barley Research, Yangzhou UniversityYangzhou, China.
Jun
J
Ma
Jiangsu Key Laboratory of Crop Genetics and Physiology, Yangzhou UniversityYangzhou, China; Co-Innovation Center for Modern Production Technology of Grain Crops, Yangzhou UniversityYangzhou, China; Key Laboratory of Plant Functional Genomics of the Ministry of Education, Yangzhou UniversityYangzhou, China; Institute of Barley Research, Yangzhou UniversityYangzhou, China.
Chao
C
Lv
Jiangsu Key Laboratory of Crop Genetics and Physiology, Yangzhou UniversityYangzhou, China; Co-Innovation Center for Modern Production Technology of Grain Crops, Yangzhou UniversityYangzhou, China; Key Laboratory of Plant Functional Genomics of the Ministry of Education, Yangzhou UniversityYangzhou, China; Institute of Barley Research, Yangzhou UniversityYangzhou, China.
RuGen
R
Xu
Hordeum vulgare L.; QTL; chasmogamous; doubled haploid; glume opening angle