Metabolic Changes Investigated by Proton NMR Spectroscopy in Cells Undergoing Oncogene-Induced Senescence.
Investigating metabolic changes during different organismal or cellular states is of increasing interest. The combination of a data-rich analytical method like mass spectrometry or NMR spectroscopy with a statistical analysis identifies metabolites that are affected by a certain stimulus. Thus, important information on the underlying molecular pathways can be obtained. Here, we describe how to investigate metabolic changes in a model of oncogene-induced senescence. The water-soluble metabolites are isolated by a chloroform-methanol treatment and subsequently analyzed by NMR spectroscopy.
Institute of Chemistry, University of Lübeck, Ratzeburger Allee 160, 23562, Lübeck, Germany.
Claudia
C
Gey
Institute of Chemistry, University of Lübeck, Ratzeburger Allee 160, 23562, Lübeck, Germany. karsten.seeger@chemie.uni-luebeck.de.
Karsten
K
Seeger
D002460:Cell Line; D002478:Cells, Cultured; D016922:Cellular Senescence / Q000235:genetics; D002794:Choline / Q000378:metabolism; D005347:Fibroblasts / Q000378:metabolism; D006801:Humans; D055442:Metabolome; D055432:Metabolomics / Q000379:methods; D009857:Oncogenes / Q000235:genetics; D066244:Proton Magnetic Resonance Spectroscopy / Q000379:methods
D002794:Choline
Cellular senescence; Choline metabolism; Metabolite extraction; Metabolomics; NMR spectroscopy