Endothelial Cell Hypertrophy and Microvascular Proliferation in Meningiomas Are Correlated with Higher Histological Grade and Shorter Progression-Free Survival.
Microvascular proliferation (MVP) is a hallmark of glioblastoma. Endothelial cell hypertrophy (ECH), also known as endothelial hyperplasia, is correlated with a shorter survival of patients with gliomas. However, the prognostic value of these 2 morphological features has not been studied in meningiomas. The aim of this study was to evaluate the prognostic value of angiogenesis in meningiomas, most notably ECH, MVP, and microvascular density, which were evaluated using immunohistochemistry with antibodies against CD34 and CD105 (a marker of neovascularization) in a series of 139 meningiomas. ECH, MVP, and CD105 immunoreactivity were significantly correlated with higher histological grades (p < 0.0001, p = 0.0004, and p = 0.0003, respectively). ECH and MVP but not CD105 immunoreactivities were significantly correlated with a shorter progression-free survival time (PFS) (p = 0.017, p = 0.021, and p = 0.137, respectively). In Cox multivariate analysis, ECH was an independent predictor of shorter PFS (p = 0.028). Therefore, ECH and MVP are markers of shorter PFS in meningiomas and are significantly correlated with grade. These findings give insight into the use of anti-angiogenic therapies. Further studies are needed to determine whether these markers could allow us to identify patients who could benefit from anti-angiogenic therapies.
From the Department of Pathology, CHRU, Nancy, France (CL, CP, MT JMC, JMV, GG); Department of Neurosurgery, CHRU, Nancy, France (FR); INSERM U1051, Institut des Neurosciences de Montpellier, Montpellier, France (FR); INSERM U954, NGERE, Faculté de Médecine de Nancy, Université de Lorraine, Vandoeuvre-lès-Nancy, France (RP, JMV, GG); Laboratory of Neuropathology, Groupe Hospitalier Pitié-Salpêtrière, Assistance Publique-Hôpitaux de Paris, Paris, France (FB, KM); INSERM U1127, CNRS UMR 7225, Université Pierre et Marie Curie-Paris 6, Institut du Cerveau et de la Moelle épinière, Paris, France (FB, KM, MK, MP); Centre de Ressources Biologiques, BB-0033-00035, CHRU, Nancy, France (JMV, GG); and Department of Neurosurgery, Groupe Hospitalier Pitié-Salpêtrière, Assistance Publique-Hôpitaux de Paris, Paris, France (MK, MP).
Catherine
C
Ling
From the Department of Pathology, CHRU, Nancy, France (CL, CP, MT JMC, JMV, GG); Department of Neurosurgery, CHRU, Nancy, France (FR); INSERM U1051, Institut des Neurosciences de Montpellier, Montpellier, France (FR); INSERM U954, NGERE, Faculté de Médecine de Nancy, Université de Lorraine, Vandoeuvre-lès-Nancy, France (RP, JMV, GG); Laboratory of Neuropathology, Groupe Hospitalier Pitié-Salpêtrière, Assistance Publique-Hôpitaux de Paris, Paris, France (FB, KM); INSERM U1127, CNRS UMR 7225, Université Pierre et Marie Curie-Paris 6, Institut du Cerveau et de la Moelle épinière, Paris, France (FB, KM, MK, MP); Centre de Ressources Biologiques, BB-0033-00035, CHRU, Nancy, France (JMV, GG); and Department of Neurosurgery, Groupe Hospitalier Pitié-Salpêtrière, Assistance Publique-Hôpitaux de Paris, Paris, France (MK, MP).
Celso
C
Pouget
From the Department of Pathology, CHRU, Nancy, France (CL, CP, MT JMC, JMV, GG); Department of Neurosurgery, CHRU, Nancy, France (FR); INSERM U1051, Institut des Neurosciences de Montpellier, Montpellier, France (FR); INSERM U954, NGERE, Faculté de Médecine de Nancy, Université de Lorraine, Vandoeuvre-lès-Nancy, France (RP, JMV, GG); Laboratory of Neuropathology, Groupe Hospitalier Pitié-Salpêtrière, Assistance Publique-Hôpitaux de Paris, Paris, France (FB, KM); INSERM U1127, CNRS UMR 7225, Université Pierre et Marie Curie-Paris 6, Institut du Cerveau et de la Moelle épinière, Paris, France (FB, KM, MK, MP); Centre de Ressources Biologiques, BB-0033-00035, CHRU, Nancy, France (JMV, GG); and Department of Neurosurgery, Groupe Hospitalier Pitié-Salpêtrière, Assistance Publique-Hôpitaux de Paris, Paris, France (MK, MP).
Fabien
F
Rech
From the Department of Pathology, CHRU, Nancy, France (CL, CP, MT JMC, JMV, GG); Department of Neurosurgery, CHRU, Nancy, France (FR); INSERM U1051, Institut des Neurosciences de Montpellier, Montpellier, France (FR); INSERM U954, NGERE, Faculté de Médecine de Nancy, Université de Lorraine, Vandoeuvre-lès-Nancy, France (RP, JMV, GG); Laboratory of Neuropathology, Groupe Hospitalier Pitié-Salpêtrière, Assistance Publique-Hôpitaux de Paris, Paris, France (FB, KM); INSERM U1127, CNRS UMR 7225, Université Pierre et Marie Curie-Paris 6, Institut du Cerveau et de la Moelle épinière, Paris, France (FB, KM, MK, MP); Centre de Ressources Biologiques, BB-0033-00035, CHRU, Nancy, France (JMV, GG); and Department of Neurosurgery, Groupe Hospitalier Pitié-Salpêtrière, Assistance Publique-Hôpitaux de Paris, Paris, France (MK, MP).
Robin
R
Pflaum
From the Department of Pathology, CHRU, Nancy, France (CL, CP, MT JMC, JMV, GG); Department of Neurosurgery, CHRU, Nancy, France (FR); INSERM U1051, Institut des Neurosciences de Montpellier, Montpellier, France (FR); INSERM U954, NGERE, Faculté de Médecine de Nancy, Université de Lorraine, Vandoeuvre-lès-Nancy, France (RP, JMV, GG); Laboratory of Neuropathology, Groupe Hospitalier Pitié-Salpêtrière, Assistance Publique-Hôpitaux de Paris, Paris, France (FB, KM); INSERM U1127, CNRS UMR 7225, Université Pierre et Marie Curie-Paris 6, Institut du Cerveau et de la Moelle épinière, Paris, France (FB, KM, MK, MP); Centre de Ressources Biologiques, BB-0033-00035, CHRU, Nancy, France (JMV, GG); and Department of Neurosurgery, Groupe Hospitalier Pitié-Salpêtrière, Assistance Publique-Hôpitaux de Paris, Paris, France (MK, MP).
Mathilde
M
Treffel
From the Department of Pathology, CHRU, Nancy, France (CL, CP, MT JMC, JMV, GG); Department of Neurosurgery, CHRU, Nancy, France (FR); INSERM U1051, Institut des Neurosciences de Montpellier, Montpellier, France (FR); INSERM U954, NGERE, Faculté de Médecine de Nancy, Université de Lorraine, Vandoeuvre-lès-Nancy, France (RP, JMV, GG); Laboratory of Neuropathology, Groupe Hospitalier Pitié-Salpêtrière, Assistance Publique-Hôpitaux de Paris, Paris, France (FB, KM); INSERM U1127, CNRS UMR 7225, Université Pierre et Marie Curie-Paris 6, Institut du Cerveau et de la Moelle épinière, Paris, France (FB, KM, MK, MP); Centre de Ressources Biologiques, BB-0033-00035, CHRU, Nancy, France (JMV, GG); and Department of Neurosurgery, Groupe Hospitalier Pitié-Salpêtrière, Assistance Publique-Hôpitaux de Paris, Paris, France (MK, MP).
Franck
F
Bielle
From the Department of Pathology, CHRU, Nancy, France (CL, CP, MT JMC, JMV, GG); Department of Neurosurgery, CHRU, Nancy, France (FR); INSERM U1051, Institut des Neurosciences de Montpellier, Montpellier, France (FR); INSERM U954, NGERE, Faculté de Médecine de Nancy, Université de Lorraine, Vandoeuvre-lès-Nancy, France (RP, JMV, GG); Laboratory of Neuropathology, Groupe Hospitalier Pitié-Salpêtrière, Assistance Publique-Hôpitaux de Paris, Paris, France (FB, KM); INSERM U1127, CNRS UMR 7225, Université Pierre et Marie Curie-Paris 6, Institut du Cerveau et de la Moelle épinière, Paris, France (FB, KM, MK, MP); Centre de Ressources Biologiques, BB-0033-00035, CHRU, Nancy, France (JMV, GG); and Department of Neurosurgery, Groupe Hospitalier Pitié-Salpêtrière, Assistance Publique-Hôpitaux de Paris, Paris, France (MK, MP).
Karima
K
Mokhtari
From the Department of Pathology, CHRU, Nancy, France (CL, CP, MT JMC, JMV, GG); Department of Neurosurgery, CHRU, Nancy, France (FR); INSERM U1051, Institut des Neurosciences de Montpellier, Montpellier, France (FR); INSERM U954, NGERE, Faculté de Médecine de Nancy, Université de Lorraine, Vandoeuvre-lès-Nancy, France (RP, JMV, GG); Laboratory of Neuropathology, Groupe Hospitalier Pitié-Salpêtrière, Assistance Publique-Hôpitaux de Paris, Paris, France (FB, KM); INSERM U1127, CNRS UMR 7225, Université Pierre et Marie Curie-Paris 6, Institut du Cerveau et de la Moelle épinière, Paris, France (FB, KM, MK, MP); Centre de Ressources Biologiques, BB-0033-00035, CHRU, Nancy, France (JMV, GG); and Department of Neurosurgery, Groupe Hospitalier Pitié-Salpêtrière, Assistance Publique-Hôpitaux de Paris, Paris, France (MK, MP).
Jean-Matthieu
JM
Casse
From the Department of Pathology, CHRU, Nancy, France (CL, CP, MT JMC, JMV, GG); Department of Neurosurgery, CHRU, Nancy, France (FR); INSERM U1051, Institut des Neurosciences de Montpellier, Montpellier, France (FR); INSERM U954, NGERE, Faculté de Médecine de Nancy, Université de Lorraine, Vandoeuvre-lès-Nancy, France (RP, JMV, GG); Laboratory of Neuropathology, Groupe Hospitalier Pitié-Salpêtrière, Assistance Publique-Hôpitaux de Paris, Paris, France (FB, KM); INSERM U1127, CNRS UMR 7225, Université Pierre et Marie Curie-Paris 6, Institut du Cerveau et de la Moelle épinière, Paris, France (FB, KM, MK, MP); Centre de Ressources Biologiques, BB-0033-00035, CHRU, Nancy, France (JMV, GG); and Department of Neurosurgery, Groupe Hospitalier Pitié-Salpêtrière, Assistance Publique-Hôpitaux de Paris, Paris, France (MK, MP).
Jean-Michel
JM
Vignaud
From the Department of Pathology, CHRU, Nancy, France (CL, CP, MT JMC, JMV, GG); Department of Neurosurgery, CHRU, Nancy, France (FR); INSERM U1051, Institut des Neurosciences de Montpellier, Montpellier, France (FR); INSERM U954, NGERE, Faculté de Médecine de Nancy, Université de Lorraine, Vandoeuvre-lès-Nancy, France (RP, JMV, GG); Laboratory of Neuropathology, Groupe Hospitalier Pitié-Salpêtrière, Assistance Publique-Hôpitaux de Paris, Paris, France (FB, KM); INSERM U1127, CNRS UMR 7225, Université Pierre et Marie Curie-Paris 6, Institut du Cerveau et de la Moelle épinière, Paris, France (FB, KM, MK, MP); Centre de Ressources Biologiques, BB-0033-00035, CHRU, Nancy, France (JMV, GG); and Department of Neurosurgery, Groupe Hospitalier Pitié-Salpêtrière, Assistance Publique-Hôpitaux de Paris, Paris, France (MK, MP).
Michel
M
Kalamarides
From the Department of Pathology, CHRU, Nancy, France (CL, CP, MT JMC, JMV, GG); Department of Neurosurgery, CHRU, Nancy, France (FR); INSERM U1051, Institut des Neurosciences de Montpellier, Montpellier, France (FR); INSERM U954, NGERE, Faculté de Médecine de Nancy, Université de Lorraine, Vandoeuvre-lès-Nancy, France (RP, JMV, GG); Laboratory of Neuropathology, Groupe Hospitalier Pitié-Salpêtrière, Assistance Publique-Hôpitaux de Paris, Paris, France (FB, KM); INSERM U1127, CNRS UMR 7225, Université Pierre et Marie Curie-Paris 6, Institut du Cerveau et de la Moelle épinière, Paris, France (FB, KM, MK, MP); Centre de Ressources Biologiques, BB-0033-00035, CHRU, Nancy, France (JMV, GG); and Department of Neurosurgery, Groupe Hospitalier Pitié-Salpêtrière, Assistance Publique-Hôpitaux de Paris, Paris, France (MK, MP).
Matthieu
M
Peyre
From the Department of Pathology, CHRU, Nancy, France (CL, CP, MT JMC, JMV, GG); Department of Neurosurgery, CHRU, Nancy, France (FR); INSERM U1051, Institut des Neurosciences de Montpellier, Montpellier, France (FR); INSERM U954, NGERE, Faculté de Médecine de Nancy, Université de Lorraine, Vandoeuvre-lès-Nancy, France (RP, JMV, GG); Laboratory of Neuropathology, Groupe Hospitalier Pitié-Salpêtrière, Assistance Publique-Hôpitaux de Paris, Paris, France (FB, KM); INSERM U1127, CNRS UMR 7225, Université Pierre et Marie Curie-Paris 6, Institut du Cerveau et de la Moelle épinière, Paris, France (FB, KM, MK, MP); Centre de Ressources Biologiques, BB-0033-00035, CHRU, Nancy, France (JMV, GG); and Department of Neurosurgery, Groupe Hospitalier Pitié-Salpêtrière, Assistance Publique-Hôpitaux de Paris, Paris, France (MK, MP).
Guillaume
G
Gauchotte
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D049109:Cell Proliferation; D018572:Disease-Free Survival; D042783:Endothelial Cells / Q000473:pathology*; D006801:Humans; D006984:Hypertrophy / Q000473:pathology; D008577:Meningeal Neoplasms / Q000473:pathology*; D008579:Meningioma / Q000473:pathology*; D055806:Microvessels / Q000473:pathology*; D008875:Middle Aged; D060787:Neoplasm Grading / Q000379:methods; D009389:Neovascularization, Pathologic / Q000473:pathology; D011897:Random Allocation; D012189:Retrospective Studies
CD105; CD34; Endothelial cell hypertrophy; Meningioma; Microvascular proliferation; Microvessel density; Vascularization.