Targetting αvβ3 and α5β1 integrins with Ecballium elaterium (L.) A. Rich. seed oil.
In the present study, the effect of Ecbalium elaterium seed oil on adhesion, migration and proliferation of human brain cancer cell line (U87) was determined. Treatment of U87 cell line with the seed oil resulted in strong inhibition of their adhesion to fibrinogen (Fg), fibronectin (Fn). It also reduced their migration and proliferation in a dose-dependent manner without being cytotoxic. Concomitantly, by using Matrigel™ assays, the oil significantly inhibited angiogenesis. The anti- tumor effect of the oil is specifically mediated by αvβ3 and α5β1 integrins. The presence of integrin antagonists in seed oil from E. elaterium could be used for the development of anticancer drugs with targeted "multi-modal" therapies combining anti-adhesif, antiproliferative, antimetastasic and anti-angiogenic, approaches.
Laboratoire des Substances Naturelles, Institut National de Recherche et d'Analyse Physico-chimique (INRAP), Sidi Thabet, 2020 Ariana, Tunisie. Electronic address: imen.touihri@gmail.com.
Imen
I
Touihri-Barakati
Laboratoire des Substances Naturelles, Institut National de Recherche et d'Analyse Physico-chimique (INRAP), Sidi Thabet, 2020 Ariana, Tunisie.
Olfa
O
Kallech-Ziri
Laboratoire des Substances Naturelles, Institut National de Recherche et d'Analyse Physico-chimique (INRAP), Sidi Thabet, 2020 Ariana, Tunisie.
Abdennacer
A
Boulila
Laboratoire des Substances Naturelles, Institut National de Recherche et d'Analyse Physico-chimique (INRAP), Sidi Thabet, 2020 Ariana, Tunisie.
Khaoula
K
Khwaldia
Faculté de Médecine de Tunis, La Rabta 1007 Tunis, Tunisie; Laboratoire des Venins et Biomolécules Thérapeutiques, Institut Pasteur de Tunis, 13, Place Pasteur, 1002 Tunis Belvédère, Tunisie.
Naziha
N
Marrakchi
Faculté des Sciences de Tunis, Campus Universitaire, Tunis El Manar, 1000 Tunis, Tunisie.
Belgacem
B
Hanchi
Laboratoire des Substances Naturelles, Institut National de Recherche et d'Analyse Physico-chimique (INRAP), Sidi Thabet, 2020 Ariana, Tunisie.
Karim
K
Hosni
Aix-Marseille Université, INSERM UMR 911, Centre de Recherche en Oncologie Biologique et Oncopharmacologie (CRO2), Faculté de Pharmacie, 13385 Marseille, France.
José
J
Luis
D001932:Brain Neoplasms / Q000098:blood supply / Q000188:drug therapy / Q000473:pathology; D002448:Cell Adhesion / Q000187:drug effects; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D019662:Cucurbitaceae / Q000737:chemistry*; D005910:Glioma / Q000098:blood supply / Q000188:drug therapy / Q000473:pathology; D006801:Humans; D039081:Integrin alpha5beta1 / Q000378:metabolism*; D039661:Integrin beta3 / Q000378:metabolism*; D009389:Neovascularization, Pathologic / Q000188:drug therapy; D010938:Plant Oils / Q000494:pharmacology* / Q000627:therapeutic use; D012639:Seeds / Q000737:chemistry*; D059008:Time-Lapse Imaging
D039081:Integrin alpha5beta1; D039661:Integrin beta3; D010938:Plant Oils
Adhesion; Angiogenesis; Ecballium elaterium; Glioma cell line; Seed oil; αvβ3 and α5β1 integrins