Genome-Wide miRNA Screening for Genes Bypassing Oncogene-Induced Senescence.
MicroRNAs are small noncoding RNAs that regulate gene expression by binding to sequences within the 3'-UTR of mRNAs. Genome-wide screens have proven powerful in associating genes with certain phenotypes or signal transduction pathways and thus are valuable tools to define gene function. Here we describe a genome-wide miRNA screening strategy to identify miRNAs that are required to bypass oncogene-induced senescence.
Musculoskeletal and Oncology Lab, Department of Orthopaedics and Rehabilitation, University of Florida, 1600 Archer Road, MSB M2-212, Gainesville, FL, 32610, USA. guijam@ufl.edu.
Maria V
MV
Guijarro
Molecular Biology of Cancer Group, Oncohematology and Genetic Department, Instituto de Biomedicina de Sevilla (IBIS/HUVR/CSIC/Universidad de Sevilla), Campus HUVR, Edificio IBIS, Avda. Manuel Siurot s/n. 41013, Sevilla, Spain. acarnero-ibis@us.es.
Amancio
A
Carnero
D002460:Cell Line; D016922:Cellular Senescence / Q000235:genetics*; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D011905:Genes, ras; D005822:Genetic Vectors / Q000235:genetics; D055106:Genome-Wide Association Study; D006801:Humans; D035683:MicroRNAs / Q000235:genetics*; D009857:Oncogenes; D034622:RNA Interference; D015203:Reproducibility of Results; D012190:Retroviridae / Q000235:genetics; D059467:Transcriptome; D014161:Transduction, Genetic
D035683:MicroRNAs
Cellular senescence; Oncogene-induced senescence; Screening; miRNA