Performance and microbial community analysis of a novel DEAMOX based on partial-denitrification and anammox treating ammonia and nitrate wastewaters.
In this study, a novel DEAMOX (DEnitrifying AMmonium OXidation) process coupling anammox with partial-denitrification generated nitrite (NO2--N) from nitrate (NO3--N) was developed for simultaneously treating ammonia (NH4+-N) and NO3--N containing wastewaters. The performance was evaluated in sequencing batch reactors (SBRs) with different carbon sources for partial-denitrification: acetate (R1) and ethanol (R2). Long-term operation (180 days) suggested that desirable nitrogen removal was achieved in both reactors. The performance maintained stably in R1 despite the seasonal decrease of temperature (29.2 °C-12.7 °C), and high nitrogen removal efficiency (NRE) of 93.6% on average was obtained with influent NO3--N to NH4+-N ratio (NO3--N/NH4+-N) of 1.0. The anammox process contributed above 95% to total nitrogen (TN) removal in R1 with the nitrate-to-nitrite transformation ratio (NTR) of 95.8% in partial-denitrification. A little lower NRE was observed in R2 with temperature dropped from 90.0% at 22.7 °C to 85.2% at 16.6 °C due to the reduced NTR (87.0%-67.0%). High-throughput sequencing analysis revealed that Thauera genera were dominant in both SBRs (accounted for 61.53% in R1 and 45.17% in R2) and possibly played a key role for partial-denitrification with high NO2--N accumulation. The Denitratisoma capable of complete denitrification (NO3--N→N2) was found in R2 that might lead to lower NTR. Furthermore, different anammox species was detected with Candidatus Brocadia and Candidatus Kuenenia in R1, and only Candidatus Kuenenia in R2.
National Engineering Laboratory for Advanced Municipal Wastewater Treatment and Reuse Technology, Key Laboratory of Beijing for Water Quality Science and Water Environment Recovory Engineering, Beijing University of Technology, Beijing 100124, China.
Rui
R
Du
State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin 150090, China.
Shenbin
S
Cao
National Engineering Laboratory for Advanced Municipal Wastewater Treatment and Reuse Technology, Key Laboratory of Beijing for Water Quality Science and Water Environment Recovory Engineering, Beijing University of Technology, Beijing 100124, China.
Baikun
B
Li
National Engineering Laboratory for Advanced Municipal Wastewater Treatment and Reuse Technology, Key Laboratory of Beijing for Water Quality Science and Water Environment Recovory Engineering, Beijing University of Technology, Beijing 100124, China.
Meng
M
Niu
National Engineering Laboratory for Advanced Municipal Wastewater Treatment and Reuse Technology, Key Laboratory of Beijing for Water Quality Science and Water Environment Recovory Engineering, Beijing University of Technology, Beijing 100124, China.
Shuying
S
Wang
National Engineering Laboratory for Advanced Municipal Wastewater Treatment and Reuse Technology, Key Laboratory of Beijing for Water Quality Science and Water Environment Recovory Engineering, Beijing University of Technology, Beijing 100124, China. Electronic address: pyz@bjut.edu.cn.
Yongzhen
Y
Peng
D000641:Ammonia; D019149:Bioreactors; D058440:Denitrification; D009566:Nitrates; D009584:Nitrogen; D010084:Oxidation-Reduction; D062065:Wastewater
D000641:Ammonia; D009566:Nitrates; D009584:Nitrogen; D062065:Wastewater
Acetate; Anammox; Ethanol; High-throughput sequencing analysis; Nitrate; Partial-denitrification