RNF167 targets Arl8B for degradation to regulate lysosome positioning and endocytic trafficking.
The protease-associated (PA) domain-containing E3 ubiquitin ligases are transmembrane proteins located in intracellular organelles such as the endoplasmic reticulum, endosomes, or lysosomes. The functional roles of these ubiquitin ligases are not well defined. To understand the function of E3 ubiquitin ligases, identification of their substrates is of critical importance. In this study, we describe a newly devised method based on proximity-dependent biotin labeling to identify substrates of ubiquitin ligases. Application of this method to RING finger protein 167 (RNF167), a member of the PA domain-containing E3 family, led to identification of Arl8B as its substrate. We demonstrated that RNF167 ubiquitinates Arl8B at the lysine residue K141 and reduces the level of the Arl8B protein. Overexpression and knockdown of RNF167 revealed its regulatory role in Arl8B-dependent lysosome positioning and endocytic trafficking to lysosomes. Furthermore, we found that the ubiquitination-defective Arl8B K141R mutant counteracts RNF167 in these cellular events. These results indicate that RNF167 plays a crucial role as an E3 ubiquitin ligase targeting Arl8B to regulate lysosome positioning and endocytic trafficking.
Department of Medical Lifesciences, The Catholic University of Korea, Seoul, Korea.
Rakesh
R
Deshar
Department of Biochemistry and Translational Research Center for Protein Function Control, Yonsei University, Seoul, Korea.
Song
0000-0002-0436-3699
S
Moon
Department of Biochemistry and Translational Research Center for Protein Function Control, Yonsei University, Seoul, Korea.
Wonjin
W
Yoo
Department of Biochemistry and Translational Research Center for Protein Function Control, Yonsei University, Seoul, Korea.
Eun-Bee
0000-0002-0880-7584
EB
Cho
Department of Medical Lifesciences, The Catholic University of Korea, Seoul, Korea.
Sungjoo K
SK
Yoon
Department of Biochemistry and Translational Research Center for Protein Function Control, Yonsei University, Seoul, Korea.
Jong-Bok
JB
Yoon
D020727:ADP-Ribosylation Factors / Q000235:genetics / Q000378:metabolism*; D001665:Binding Sites / Q000235:genetics; D004705:Endocytosis; D011992:Endosomes / Q000378:metabolism*; D006367:HeLa Cells; D006801:Humans; D015151:Immunoblotting; D008239:Lysine / Q000235:genetics / Q000378:metabolism; D008247:Lysosomes / Q000378:metabolism*; D018613:Microscopy, Confocal; D009154:Mutation; D021381:Protein Transport; D059748:Proteolysis; D034622:RNA Interference; D044767:Ubiquitin-Protein Ligases / Q000235:genetics / Q000378:metabolism*; D054875:Ubiquitination
C511801:ARL8B protein, human; C108062:TTC3 protein, human; D044767:Ubiquitin-Protein Ligases; D020727:ADP-Ribosylation Factors; D008239:Lysine
Arl8B; RNF167; endocytosis; lysosome; proteolysis