A multi-step peptidolytic cascade for amino acid recovery in chloroplasts.
Plastids (including chloroplasts) are subcellular sites for a plethora of proteolytic reactions, required in functions ranging from protein biogenesis to quality control. Here we show that peptides generated from pre-protein maturation within chloroplasts of Arabidopsis thaliana are degraded to amino acids by a multi-step peptidolytic cascade consisting of oligopeptidases and aminopeptidases, effectively allowing the recovery of single amino acids within these organelles.
Department of Biochemistry and Biophysics, Stockholm University, Arrhenius Laboratories for Natural Sciences, Stockholm, Sweden.
Pedro F
http://orcid.org/0000-0001-8638-7477
PF
Teixeira
Department of Biochemistry and Biophysics, Stockholm University, Arrhenius Laboratories for Natural Sciences, Stockholm, Sweden.
Beata
B
Kmiec
Clinical Proteomics Mass Spectrometry, Department of Oncology-Pathology, Science for Life Laboratory and Karolinska Institutet, Stockholm, Sweden.
Rui M M
RM
Branca
ARC Centre of Excellence in Plant Energy Biology, University of Western Australia, Crawley, Western Australia, Australia.
Monika W
http://orcid.org/0000-0002-3689-6158
MW
Murcha
Division of Bioorganic Chemistry, Department of Chemistry, Wroclaw University of Technology, Wroclaw, Poland.
Anna
A
Byzia
ARC Centre of Excellence in Plant Energy Biology, University of Western Australia, Crawley, Western Australia, Australia.
Aneta
A
Ivanova
ARC Centre of Excellence in Plant Energy Biology, Department of Animal, Plant and Soil Science, School of Life Science, La Trobe University, Victoria, Australia.
James
J
Whelan
Division of Bioorganic Chemistry, Department of Chemistry, Wroclaw University of Technology, Wroclaw, Poland.
Marcin
M
Drag
Clinical Proteomics Mass Spectrometry, Department of Oncology-Pathology, Science for Life Laboratory and Karolinska Institutet, Stockholm, Sweden.
Janne
J
Lehtiö
Department of Biochemistry and Biophysics, Stockholm University, Arrhenius Laboratories for Natural Sciences, Stockholm, Sweden.
Elzbieta
E
Glaser
D000596:Amino Acids / Q000378:metabolism*; D017360:Arabidopsis / Q000166:cytology*; D002736:Chloroplasts / Q000378:metabolism*; D010447:Peptide Hydrolases / Q000378:metabolism*; D010455:Peptides / Q000737:chemistry / Q000378:metabolism*; D059748:Proteolysis
D000596:Amino Acids; D010455:Peptides; D010447:Peptide Hydrolases