Biodegradable double-targeted PTX-mPEG-PLGA nanoparticles for ultrasound contrast enhanced imaging and antitumor therapy in vitro.
A porous-structure nano-scale ultrasound contrast agent (UCA) was made of monomethoxypoly (ethylene glycol)-poly (lactic-co-glycolic acid) (mPEG-PLGA), and modified by double-targeted antibody: anti-carcinoembryonic antigen (CEA) and anti-carbohydrate antigen 19-9 (CA19-9), as a double-targeted nanoparticles (NPs). Anti-tumor drug paclitaxel (PTX) was encapsulated in the double-targeted nanoparticles (NPs). The morphor and release curve were characterized. We verified a certain anticancer effect of PTX-NPs through cytotoxicity experiments. The cell uptake result showed much more NPs may be facilitated to ingress the cells or tissues with ultrasound (US) or ultrasound targeted microbubble destruction (UTMD) transient sonoporation in vitro. Ultrasound contrast-enhanced images in vitro and in vivo were investigated. Compared with SonoVue, the NPs prolonged imaging time in rabbit kidneys and tumor of nude mice, which make it possible to further enhance anti-tumor effects by extending retention time in the tumor region. The novel double-targeted NPs with the function of ultrasound contrast enhanced imaging and anti-tumor therapy can be a promising way in clinic.
Department of Ultrasound, Songjiang Hospital Affiliated to The First People's Hospital of Shanghai Jiao tong University, Shanghai 201600, China.|Department of Ultrasound, Shanghai First People's Hospital Affiliated to Shanghai Jiao tong University School of Medicine, Shanghai 200080, China.|Department of Ultrasound, Shanghai East Hospital Affiliated to Tong ji University, Shanghai 200120, China.
Jing
J
Ma
State Key Laboratory of Oncogenes and Related Genes, Shanghai Cancer Institute, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200032, P. R. China.
Ming
M
Shen
Huai'an First People's Hospital, Nanjing Medical University, Jiangsu 223001, China.
Chang Song
CS
Xu
State Key Laboratory of Oncogenes and Related Genes, Shanghai Cancer Institute, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200032, P. R. China.
Ying
Y
Sun
State Key Laboratory of Oncogenes and Related Genes, Shanghai Cancer Institute, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200032, P. R. China.
You Rong
YR
Duan
Department of Ultrasound, Shanghai First People's Hospital Affiliated to Shanghai Jiao tong University School of Medicine, Shanghai 200080, China.
Lian Fang
LF
Du
D020341:Absorbable Implants; D000068196:Albumin-Bound Paclitaxel / Q000008:administration & dosage* / Q000493:pharmacokinetics* / Q000627:therapeutic use; D000818:Animals; D000970:Antineoplastic Agents / Q000008:administration & dosage* / Q000493:pharmacokinetics; D045744:Cell Line, Tumor; D003287:Contrast Media / Q000138:chemical synthesis / Q000737:chemistry; D004337:Drug Carriers / Q000138:chemical synthesis / Q000737:chemistry / Q000493:pharmacokinetics / Q000627:therapeutic use; D016503:Drug Delivery Systems; D006801:Humans; D008422:Materials Testing; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D053758:Nanoparticles / Q000737:chemistry; D010190:Pancreatic Neoplasms / Q000175:diagnosis / Q000188:drug therapy / Q000378:metabolism / Q000473:pathology; D010316:Particle Size; D011091:Polyesters / Q000138:chemical synthesis / Q000737:chemistry* / Q000493:pharmacokinetics; D011092:Polyethylene Glycols / Q000138:chemical synthesis / Q000737:chemistry* / Q000493:pharmacokinetics; D011817:Rabbits; D014463:Ultrasonography / Q000379:methods*; D023041:Xenograft Model Antitumor Assays
D000068196:Albumin-Bound Paclitaxel; D000970:Antineoplastic Agents; D003287:Contrast Media; D004337:Drug Carriers; D011091:Polyesters; C558447:methoxypolyethyleneglycol-poly(lactic-co-glycolic acid); D011092:Polyethylene Glycols
antitumor; double-targeted; nanoparticles; pancreatic cancer; ultrasound contrast enhanced imaging