Rapid HPLC Quantification Approach for Detection of Active Constituents in Modern Combinatorial Formula, San-Huang-Xie-Xin-Tang (SHXXT).
San-Huang-Xie-Xin-Tang (SHXXT), one of the most important traditional Chinese medicinal formulas, is comprised by three herbal medicines, the rhizome ofRheum officinale[orRheum tanguticum(Polygonaceae) (Dahuang in Chinese)], the root ofScutellaria baicalensis(Labiatae) (Huangqin in Chinese), and the rhizome ofCoptis chinensis(Ranunculaceae) (Huanglian in Chinese) in the ratios of 2:1:1 or 1:1:1. This study is aimed to quantitate and qualify of SHXXT, by a rapid, convenient, and effective HPLC-PDA approach associated with LC-MS technique. Of which method, nine chosen major bioactive components in SHXXT, including aloe-emodin (Ale), baicalin (Ba), berberine (Be), coptisine (Co), palmatine (Pa), resveratroloside (Res), rhein (Rh), sennoside A (Se-A), and wogonin (Wo), were evaluated within 30 min. The nine chemical markers were monitored in a high sensitivity with a low detection limit of 0.01-0.55 μg/mL and the correlation coefficient of the regression curve revealed a good linearity withR2> 0.99. Moreover, the extraction solution system and the HPLC elution conditions were also optimized in the present study. This present developed protocol was then successfully applied to quantify nine chemical markers of 10 SHXXT products from eight Taiwanese TCM pharmaceutical companies. In quantitative results,Reswas found as the major compound in SHXXT-1~5 and 8 with significantly higher amounts than those in other products, indicating the products SHXXT-1~5 and 8 may useR. tanguticumas the raw material, which possessed a higher concentration of the bioactive compositionRes, instead ofR. officinale. Simultaneously,Ale,Rh, andWowere < 2% in these 10 products. Different chemical profiles of commercial products indicated that, probably, each product with the same named formula might be regarded as a sole medicine and need to be investigated individually. Importantly, it is never too much to emphasize the importance of quality control in TCM development.
Chinese Medicine Research and Development Center, China Medical University Hospital Taichung, Taiwan.
Tung-Ying
TY
Wu
Graduate Institute of Natural Products, College of Pharmacy, Kaohsiung Medical UniversityKaohsiung, Taiwan; Center for Infectious Disease and Cancer Research, Kaohsiung Medical UniversityKaohsiung, Taiwan; Cancer Center, Kaohsiung Medical University HospitalKaohsiung, Taiwan; Research Center for Environmental Medicine, Kaohsiung Medical UniversityKaohsiung, Taiwan.
Fang-Rong
FR
Chang
Graduate Institute of Natural Products, College of Pharmacy, Kaohsiung Medical University Kaohsiung, Taiwan.
Jing-Ru
JR
Liou
Graduate Institute of Natural Products, College of Pharmacy, Kaohsiung Medical University Kaohsiung, Taiwan.
I-Wen
IW
Lo
Department of Pharmacy, Kaohsiung Medical University Hospital Kaohsiung, Taiwan.
Tang-Chia
TC
Chung
Department of Pharmacy, Kaohsiung Medical University Hospital Kaohsiung, Taiwan.
Li-Yao
LY
Lee
Graduate Institute of Natural Products, College of Pharmacy, Kaohsiung Medical University Kaohsiung, Taiwan.
Chun-Chen
CC
Chi
Graduate Institute of Natural Products, College of Pharmacy, Kaohsiung Medical University Kaohsiung, Taiwan.
Ying-Chi
YC
Du
Graduate Institute of Natural Products, College of Pharmacy, Kaohsiung Medical University Kaohsiung, Taiwan.
Man-Hon
MH
Wong
Graduate Institute of Medical Genetics, College of Medicine, Kaohsiung Medical University Kaohsiung, Taiwan.
Suh-Hang Hank
SH
Juo
Department of Pharmacy, Kaohsiung Medical University Hospital Kaohsiung, Taiwan.
Chun-Chen
CC
Lee
Chinese Medicine Research and Development Center, China Medical University HospitalTaichung, Taiwan; Graduate Institute of Natural Products, College of Pharmacy, Kaohsiung Medical UniversityKaohsiung, Taiwan; School of Pharmacy, College of Pharmacy, China Medical UniversityTaichung, Taiwan; Research Center for Chinese Herbal Medicine, China Medicinal UniversityTaichung, Taiwan; Center for Molecular Medicine, China Medical University HospitalTaichung, Taiwan.
Yang-Chang
YC
Wu
Coptis chinensis; HPLC; Rheum officinale; Rheum tanguticum; San-Huang-Xie-Xin-Tang (SHXXT); Scutellaria baicalensis; quality control; quantification