Production of nanocellulose in miniature-bioreactor: Optimization and characterization.
Bacterial cellulose (BC) is a very fascinating microbial biopolymer which is mainly produced by Gluconacetobacter xylinum. Optimization of BC production by G. xylinum was performed based on scale-down studies in miniature-bioreactor and response surface methodology in which the optimum pH value (6.5) and shaking rate (50 rpm) were obtained. The static culture condition for BC production has newly been defined. Nanostructure of BC includes nanofibers up to (60 nm) and nanoporosity up to (265 nm) was observed by scanning electron microscopy. By Fourier transform infrared spectroscopy study, the most expected BC interaction is nucleophilic interaction. MTT assay showed high biocompatibility. Appropriate mechanical strength (0.37 MPa) and Young's modulus (3.36 MPa) evinced BC scaffold utilization for skin tissue. The results indicate that BC sheets can be utilized in biomedical application and nanotechnology approaches.
a Department of Life Science Engineering, Faculty of New Science and Technology , University of Tehran , Tehran , Iran.
Sepideh
S
Khazeni
a Department of Life Science Engineering, Faculty of New Science and Technology , University of Tehran , Tehran , Iran.
Ashrafalsadat
A
Hatamian-Zarmi
a Department of Life Science Engineering, Faculty of New Science and Technology , University of Tehran , Tehran , Iran.
Fatemeh
F
Yazdian
b Department of Chemical Engineering, Faculty of Petroleum and Petrochemical Engineering , Hakim Sabzevari University , Sabzevar , Iran.
Zahra Beagom
ZB
Mokhtari-Hosseini
a Department of Life Science Engineering, Faculty of New Science and Technology , University of Tehran , Tehran , Iran.
Bahman
B
Ebrahimi-Hosseinzadeh
a Department of Life Science Engineering, Faculty of New Science and Technology , University of Tehran , Tehran , Iran.
Behnam
B
Noorani
c Faculty of Chemical Engineering, College of Engineering , University of Tehran , Tehran , Iran.|d Research Center for New Technologies in Life Science Engineering , University of Tehran , Tehran , Iran.
Ghassem
G
Amoabedini
e Department of Microbiology, Faculty of Biological Science , Alzahra University , Tehran , Iran.
Mohammad Reza
MR
Soudi
D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry / Q000378:metabolism*; D019149:Bioreactors; D002460:Cell Line; D002470:Cell Survival; D002482:Cellulose / Q000737:chemistry / Q000378:metabolism* / Q000648:ultrastructure; D055119:Elastic Modulus; D041822:Gluconacetobacter xylinus / Q000737:chemistry / Q000378:metabolism*; D007218:Industrial Microbiology; D051379:Mice; D057139:Nanofibers / Q000737:chemistry / Q000648:ultrastructure; D036103:Nanotechnology; D017550:Spectroscopy, Fourier Transform Infrared; D013718:Tensile Strength
D001672:Biocompatible Materials; D002482:Cellulose
Bacterial cellulose; Gluconacetobacter xylinum; miniature-bioreactor; optimization; static culture