Characterization and enzymatic hydrolysis of hydrothermally treated β-1,3-1,6-glucan from Aureobasidium pullulans.
The chemical structure of hydrothermally treated β-1,3-1,6-glucan from Aureobasidium pullulans was characterized using techniques such as gas chromatography/mass spectrometry (GC/MS) and nuclear magnetic resonance (NMR). The chemical shifts of anomeric carbons observed in the13C-NMR spectra suggested the presence of single flexible chains of polysaccharide in the sample. β-1,3-1,6-Glucan from A. pullulans became water-soluble, with an average molecular weight of 128,000 Da after hydrothermal treatment, and the solubility in water was approximately 10% (w/w). Sample (3% w/v) was completely hydrolyzed to glucose by enzymatic reaction with Lysing enzymes from Trichoderma harzianum. Gentiobiose (Glcβ1 → 6Glc) and glucose were released as products during the reaction, and the maximum yield of gentiobiose was approximately 70% (w/w). The molar ratio of gentiobiose to glucose after 1 h reaction suggested that the sample is likely highly branched. Sample (3% w/v) was also hydrolyzed to glucose by Uskizyme from Trichoderma sp., indicating that it is very sensitive to enzymatic hydrolysis.
Itochu Sugar Co. Ltd, 3 Tamatsuura, Hekinan-shi, Aichi, 447-8506, Japan.|The Interdisciplinary Graduate School of Agriculture and Engineering, University of Miyazaki, 1-1 Gakuen Kibanadai Nishi, Miyazaki-shi, Miyazaki, 889-2192, Japan.
Katsuki
K
Hirabayashi
Itochu Sugar Co. Ltd, 3 Tamatsuura, Hekinan-shi, Aichi, 447-8506, Japan.
Nobuhiro
N
Kondo
The Interdisciplinary Graduate School of Agriculture and Engineering, University of Miyazaki, 1-1 Gakuen Kibanadai Nishi, Miyazaki-shi, Miyazaki, 889-2192, Japan. shayashi@cc.miyazaki-u.ac.jp.
Sachio
S
Hayashi
D005656:Fungal Proteins / Q000378:metabolism*; D008401:Gas Chromatography-Mass Spectrometry; D005936:Glucans / Q000737:chemistry*; D006868:Hydrolysis; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D008970:Molecular Weight; D004718:Saccharomycetales / Q000201:enzymology* / Q000378:metabolism; D012995:Solubility; D014867:Water / Q000737:chemistry
D005656:Fungal Proteins; D005936:Glucans; C033671:epiglucan; D014867:Water
Aureobasidium pullulans; Chemical structure; Enzymatic hydrolysis; Hydrothermal treatment; β-1,3–1,6-Glucan