Molecular Role of EGFR-MAPK Pathway in Patchouli Alcohol-Induced Apoptosis and Cell Cycle Arrest on A549 CellsIn VitroandIn Vivo.
Nowadays, chemotherapy is still the main effective treatment for cancer. Herb prescriptions containingPogostemon cablin Benth(also known as "Guang-Huo-Xiang") have been widely used in Chinese medicine today. In our research, we found that patchouli alcohol, a compound isolated from the oil ofPogostemon cablin Benth, exerted antitumor ability against human lung cancer A549 cells ability bothin vitroandin vivo. MTT assay was used to assess cell viability. Hoechst 33342 staining and TUNEL cover glass staining provided the visual evidence of apoptosis. Caspase activity measurement showed that patchouli alcohol activated caspase 9 and caspase 3 of mitochondria-mediated apoptosis. Consistently, patchouli alcohol inhibited the xenograft tumorin vivo. Further investigation of the underlying molecular mechanism showed that MAPK and EGFR pathway might contribute to the antitumor effect of patchouli alcohol. Our study proved that patchouli alcohol might be able to serve as a novel antitumor compound in the clinical treatment of lung cancer.
Department of Traditional Chinese Medicine, Huadong Hospital, Fudan University, Shanghai 200040, China.
XinGang
0000-0003-0893-7263
X
Lu
Department of Oncology, Baoshan Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 201999, China.
Liu
L
Yang
Department of Respiratory, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200030, China.
ChengHua
C
Lu
Department of Traditional Chinese Medicine, Wujing Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200241, China.
ZhenYu
Z
Xu
Department of Traditional Chinese Medicine, Huadong Hospital, Fudan University, Shanghai 200040, China.
HongFu
H
Qiu
Department of Rehabilitation, The Second People Huaian Hospital, Xuzhou Medical College, Huaian 223002, China.
JiaJia
0000-0001-7515-3958
J
Wu
Department of Oncology, Huadong Hospital, Fudan University, Shanghai 200040, China.
JingWen
J
Wang
Department of Traditional Chinese Medicine, Huadong Hospital, Fudan University, Shanghai 200040, China.
JiaFeng
0000-0003-3972-878X
J
Tong
Department of Traditional Chinese Medicine, Huadong Hospital, Fudan University, Shanghai 200040, China.
Yin
Y
Zhu
Department of Pharmacy, Huadong Hospital, Fudan University, Shanghai 200040, China.
Jie
0000-0001-7330-2446
J
Shen
D000072283:A549 Cells; D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D006801:Humans; D008175:Lung Neoplasms / Q000188:drug therapy* / Q000473:pathology; D008516:Medicine, Chinese Traditional; D051379:Mice; D000070177:Pogostemon / Q000737:chemistry; D012717:Sesquiterpenes / Q000008:administration & dosage* / Q000737:chemistry; D023041:Xenograft Model Antitumor Assays
D012717:Sesquiterpenes; C052983:patchouli alcohol