Hyperspectral imaging analysis of a photonic crystal bead array for multiplex bioassays.
For multiplex bioassays, one effective strategy is to employ microfluidic chips based on an array of photonic crystal beads (PCBs) that are encoded by their characteristic reflection spectrum (CRS). In this paper, we report a hyperspectral imaging system and algorithms for the high throughput decoding of a PCB array and subsequent detection. The results showed that the decoding accuracy of up to ∼500 PCBs is 98.56% with an excellent ability to extract low-intensity fluorescence intensities. The results also demonstrated hyperspectral imaging techniques which can simultaneously obtain both spatial and spectral information as powerful tools in the analysis of multiplex bioassays or microfluidic chips.
State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing, 210096, China and Laboratory of Environment and Biosafety Research Institute of Southeast University in Suzhou, Suzhou 215123, China.
Xiangwei
X
Zhao
State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing, 210096, China and Laboratory of Environment and Biosafety Research Institute of Southeast University in Suzhou, Suzhou 215123, China.
Tengfei
T
Ma
State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing, 210096, China and Laboratory of Environment and Biosafety Research Institute of Southeast University in Suzhou, Suzhou 215123, China.
Zhaoyu
http://orcid.org/0000-0002-9683-3538
Z
Zeng
State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing, 210096, China and Department of Oncology, Zhongda Hospital, Medical School, Southeast University, Nanjing, Jiangsu Province 210009, P.R. China.
Shiya
S
Zheng
State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing, 210096, China and Laboratory of Environment and Biosafety Research Institute of Southeast University in Suzhou, Suzhou 215123, China.
Zhongze
Z
Gu
D001681:Biological Assay; D008027:Light; D044085:Microfluidics; D017785:Photons; D013057:Spectrum Analysis