Engineering Yarrowia lipolytica for poly-3-hydroxybutyrate production.
Strains of Yarrowia lipolytica were engineered to express the poly-3-hydroxybutyrate (PHB) biosynthetic pathway. The genes for β-ketothiolase, NADPH-dependent acetoacetyl-CoA reductase, and PHB synthase were cloned and inserted into the chromosome of Y. lipolytica. In shake flasks, the engineered strain accumulated PHB to 1.50 and 3.84% of cell dry weight in complex medium supplemented with glucose and acetate as carbon source, respectively. In fed-batch fermentation using acetate as sole carbon source, 7.35 g/l PHB (10.2% of cell dry weight) was produced. Selection of Y. lipolytica as host for PHB synthesis was motivated by the fact that this organism is a good lipids producer, which suggests robust acetyl-CoA supply also the precursor of the PHB pathway. Acetic acid could be supplied by gas fermentation, anaerobic digestion, and other low-cost supply route.
Department of Chemical Engineering, Massachusetts Institute of Technology, Room 56-469, 77 Massachusetts Avenue, Cambridge, MA, 02139, USA.|Beijing Key Laboratory of Bioprocess, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing, 100029, People's Republic of China.
Zheng-Jun
ZJ
Li
Department of Chemical Engineering, Massachusetts Institute of Technology, Room 56-469, 77 Massachusetts Avenue, Cambridge, MA, 02139, USA.
Kangjian
K
Qiao
Department of Chemical Engineering, Massachusetts Institute of Technology, Room 56-469, 77 Massachusetts Avenue, Cambridge, MA, 02139, USA.
Nian
N
Liu
Department of Chemical Engineering, Massachusetts Institute of Technology, Room 56-469, 77 Massachusetts Avenue, Cambridge, MA, 02139, USA. gregstep@mit.edu.
Gregory
G
Stephanopoulos
D000105:Acetyl Coenzyme A / Q000235:genetics / Q000378:metabolism; D000429:Alcohol Oxidoreductases / Q000235:genetics / Q000378:metabolism; D019149:Bioreactors; D053898:Biosynthetic Pathways; D060846:Cell Engineering; D003470:Culture Media / Q000737:chemistry; D005285:Fermentation; D005818:Genetic Engineering; D006885:Hydroxybutyrates / Q000378:metabolism*; D007218:Industrial Microbiology; D009249:NADP / Q000235:genetics / Q000378:metabolism; D011091:Polyesters / Q000378:metabolism*; D012331:RNA, Fungal / Q000235:genetics; D025062:Yarrowia / Q000235:genetics* / Q000378:metabolism; D000094122:Polyhydroxybutyrates
D000105:Acetyl Coenzyme A; D000429:Alcohol Oxidoreductases; D003470:Culture Media; D006885:Hydroxybutyrates; D009249:NADP; D011091:Polyesters; D012331:RNA, Fungal; C003182:poly-beta-hydroxybutyrate; D000094122:Polyhydroxybutyrates; C023744:acetoacetyl-CoA reductase
Acetate; Metabolic engineering; PHB; Poly-3-hydroxybutyrate; Yarrowia lipolytica; phaCAB