In Vivo Follow-up of Brain Tumor Growth via Bioluminescence Imaging and Fluorescence Tomography.
Reporter gene-based strategies are widely used in experimental oncology. Bioluminescence imaging (BLI) using the firefly luciferase (Fluc) as a reporter gene and d-luciferin as a substrate is currently the most widely employed technique. The present paper compares the performances of BLI imaging with fluorescence imaging using the near infrared fluorescent protein (iRFP) to monitor brain tumor growth in mice. Fluorescence imaging includes fluorescence reflectance imaging (FRI), fluorescence diffuseopticaltomography (fDOT), and fluorescence molecular Imaging (FMT®). A U87 cell line was genetically modified for constitutive expression of both the encoding Fluc and iRFP reporter genes and assayed for cell, subcutaneous tumor and brain tumor imaging. On cultured cells, BLI was more sensitive than FRI; in vivo, tumors were first detected by BLI. Fluorescence of iRFP provided convenient tools such as flux cytometry, direct detection of the fluorescent protein on histological slices, and fluorescent tomography that allowed for 3D localization and absolute quantification of the fluorescent signal in brain tumors.
Molecular Imaging and Innovative Therapy in Oncology (IMOTION), EA 7435, University of Bordeaux, Bordeaux 33076, France. coralie.genevois@u-bordeaux.fr.
Coralie
C
Genevois
Molecular Imaging and Innovative Therapy in Oncology (IMOTION), EA 7435, University of Bordeaux, Bordeaux 33076, France. hugues.loiseau@chu-bordeaux.fr.
Hugues
H
Loiseau
Molecular Imaging and Innovative Therapy in Oncology (IMOTION), EA 7435, University of Bordeaux, Bordeaux 33076, France. franck.couillaud@u-bordeaux.fr.
Franck
F
Couillaud
D000818:Animals; D001932:Brain Neoplasms / Q000175:diagnosis* / Q000473:pathology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D003952:Diagnostic Imaging / Q000379:methods*; D005453:Fluorescence; D006801:Humans; D008163:Luminescent Measurements / Q000379:methods*; D008164:Luminescent Proteins / Q000378:metabolism; D016688:Mice, Inbred NOD; D016513:Mice, SCID; D061848:Optical Imaging; D041622:Tomography, Optical / Q000379:methods*
D008164:Luminescent Proteins
bioluminescence; cancer; fluorescence tomography; glioblastoma; optical imaging; reporter gene