Changes in gene expression and catalase activity in Oryza sativa L. under abiotic stress.
Different rice (Oryza sativa L.) genotypes were subjected to high salinity and low temperature (150 mM NaCl and 13°C, respectively) for 0, 6, 24, 48, or 72 h. We evaluated the simultaneous expression of the genes OsCATA, OsCATB, and OsCATC, correlated gene expression with enzyme activity, and verified the regulation of these genes through identification of cis-elements in the promoter region. The hydrogen peroxide content increased in a tolerant genotype and decreased in a sensitive genotype under both stress conditions. Lipid peroxidation increased in the tolerant genotype when exposed to cold, and in the sensitive genotype when exposed to high salinity. Catalase activity significantly increased in both genotypes when subjected to 13°C. In the tolerant genotype, OsCATA and OsCATB were the most responsive to high salinity and cold, while in the sensitive genotype, OsCATA and OsCATC responded positively to saline stress, as did OsCATA and OsCATB to low temperature. Cis-element analysis identified different regulatory sequences in the catalase promoter region of each genotype. The sensitive genotype maintained a better balance between hydrogen oxyacid levels, catalase activity, and lipid peroxidation under low temperature than the resistant genotype. OsCATA and OsCATB were the most responsive in the salt-tolerant genotype to cold, OsCATA and OsCATC were the most responsive to saline stress, and OsCATA and OsCATB were the most responsive to chilling stress in the sensitive genotype. There were positive correlations between catalase activity and OsCATB expression in the tolerant genotype under saline stress and in the sensitive genotype under cold stress.
Departmento de Botânico, Universidade Federal de Pelotas, Pelotas, RS, Brasil.
I L
IL
Vighi
Departmento de Botânico, Universidade Federal de Pelotas, Pelotas, RS, Brasil.
L C
LC
Benitez
Departmento de Botânico, Universidade Federal de Pelotas, Pelotas, RS, Brasil.
M N
MN
do Amaral
Departmento de Botânico, Universidade Federal de Pelotas, Pelotas, RS, Brasil.
P A
PA
Auler
Departmento de Botânico, Universidade Federal de Pelotas, Pelotas, RS, Brasil.
G P
GP
Moraes
Departmento de Botânico, Universidade Federal de Pelotas, Pelotas, RS, Brasil.
G S
GS
Rodrigues
Departamento de Fitotecnia, Universidade Federal de Pelotas, Pelotas, RS, Brasil.
L C
LC
da Maia
Departmento de Botânico, Universidade Federal de Pelotas, Pelotas, RS, Brasil.
L S
LS
Pinto
Departmento de Botânico, Universidade Federal de Pelotas, Pelotas, RS, Brasil jacirabraga@hotmail.com.
E J B
EJ
Braga
D002374:Catalase / Q000235:genetics* / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D006861:Hydrogen Peroxide / Q000378:metabolism; D015227:Lipid Peroxidation / Q000235:genetics; D008315:Malondialdehyde / Q000378:metabolism; D012275:Oryza / Q000201:enzymology* / Q000235:genetics*; D018515:Plant Leaves / Q000201:enzymology / Q000235:genetics / Q000502:physiology; D011401:Promoter Regions, Genetic / Q000235:genetics; D054712:Salinity; D013312:Stress, Physiological / Q000235:genetics*; D013696:Temperature; D013997:Time Factors
D008315:Malondialdehyde; D006861:Hydrogen Peroxide; D002374:Catalase