Nondestructive imaging of fiber structure in articular cartilage using optical polarization tractography.
Collagen fiber orientation plays an important role in determining the structure and function of the articular cartilage. However, there is currently a lack of nondestructive means to image the fiber orientation from the cartilage surface. The purpose of this study is to investigate whether the newly developed optical polarization tractography (OPT) can image fiber structure in articular cartilage. OPT was applied to obtain the depth-dependent fiber orientation in fresh articular cartilage samples obtained from porcine phalanges. For comparison, we also obtained collagen fiber orientation in the superficial zone of the cartilage using the established split-line method. The direction of each split-line was quantified using image processing. The orientation measured in OPT agreed well with those obtained from the split-line method. The correlation analysis of a total of 112 split-lines showed a greater than 0.9 coefficient of determination (R2) between the split-line results and OPT measurements obtained between 40 and 108???m in depth. In addition, the thickness of the superficial layer can also be assessed from the birefringence images obtained in OPT. These results support that OPT provides a nondestructive way to image the collagen fiber structure in articular cartilage. This technology may be valuable for both basic cartilage research and clinical orthopedic applications.
University of Missouri, Department of Bioengineering, 1406 E. Rollins Street, Columbia 65211, United States.
Xuan
X
Yao
University of Missouri, Department of Bioengineering, 1406 E. Rollins Street, Columbia 65211, United States.
Yuanbo
Y
Wang
University of Missouri, Department of Bioengineering, 1406 E. Rollins Street, Columbia 65211, United States.
Mohammadreza
M
Ravanfar
University of Missouri, Department of Bioengineering, 1406 E. Rollins Street, Columbia 65211, United StatesbUniversity of Missouri, Department of Orthopedic Surgery, 1100 Virginia Avenue, Columbia 65212, United States.
Ferris M
FM
Pfeiffer
University of Missouri, Department of Bioengineering, 1406 E. Rollins Street, Columbia 65211, United StatescUniversity of Missouri, Department of Molecular Microbiology and Immunology, One Hospital Drive, Columbia 65212, United States.
Dongsheng
D
Duan
University of Missouri, Department of Bioengineering, 1406 E. Rollins Street, Columbia 65211, United States.
Gang
G
Yao
D000818:Animals; D002358:Cartilage, Articular / Q000000981:diagnostic imaging*; D056324:Diffusion Tensor Imaging / Q000379:methods*; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D013552:Swine; D050277:Toe Phalanges / Q000000981:diagnostic imaging; D041623:Tomography, Optical Coherence