Blue Light- and Low Temperature-Regulated COR27 and COR28 Play Roles in the Arabidopsis Circadian Clock.
Light and temperature are two key environmental signals that profoundly affect plant growth and development, but underlying molecular mechanisms of how light and temperature signals affect the circadian clock are largely unknown. Here, we report that COR27 and COR28 are regulated not only by low temperatures but also by light signals. COR27 and COR28 are negative regulators of freezing tolerance but positive regulators of flowering, possibly representing a trade-off between freezing tolerance and flowering. Furthermore, loss-of-function mutations in COR27 and COR28 result in period lengthening of various circadian output rhythms and affect central clock gene expression. Also, the cor27 cor28 double mutation affects the pace of the circadian clock. Additionally, COR27 and COR28 are direct targets of CCA1, which represses their transcription via chromatin binding. Finally, we report that COR27 and COR28 bind to the chromatin of TOC1 and PRR5 to repress their transcription, suggesting that their effects on rhythms are in part due to their regulation of TOC1 and PRR5 These data demonstrate that blue light and low temperature-regulated COR27 and COR28 regulate the circadian clock as well as freezing tolerance and flowering time.
National Key Laboratory of Plant Molecular Genetics, CAS Center for Excellence in Molecular Plant Sciences, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 200032 Shanghai, P.R. China.
Xu
X
Li
National Key Laboratory of Plant Molecular Genetics, CAS Center for Excellence in Molecular Plant Sciences, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 200032 Shanghai, P.R. China.|University of Chinese Academy of Sciences, Shanghai 200032, P.R. China.
Dingbang
D
Ma
Department of Molecular, Cell, and Developmental Biology, University of California, Los Angeles, California 90095.
Sheen X
SX
Lu
National Key Laboratory of Plant Molecular Genetics, CAS Center for Excellence in Molecular Plant Sciences, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 200032 Shanghai, P.R. China.|University of Chinese Academy of Sciences, Shanghai 200032, P.R. China.
Xinyi
X
Hu
University of Chinese Academy of Sciences, Shanghai 200032, P.R. China.|Chinese Academy of Sciences Center for Excellence in Molecular Plant Sciences, Shanghai Center for Plant Stress Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 201602 Shanghai, P.R. China.
Rongfeng
0000-0002-5295-6261
R
Huang
National Key Laboratory of Plant Molecular Genetics, CAS Center for Excellence in Molecular Plant Sciences, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 200032 Shanghai, P.R. China.|University of Chinese Academy of Sciences, Shanghai 200032, P.R. China.
Tong
0000-0003-4506-2195
T
Liang
Chinese Academy of Sciences Center for Excellence in Molecular Plant Sciences, Shanghai Center for Plant Stress Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 201602 Shanghai, P.R. China.
Tongda
T
Xu
Department of Molecular, Cell, and Developmental Biology, University of California, Los Angeles, California 90095.
Elaine M
EM
Tobin
National Key Laboratory of Plant Molecular Genetics, CAS Center for Excellence in Molecular Plant Sciences, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 200032 Shanghai, P.R. China htliu@sibs.ac.cn.
Hongtao
0000-0002-6363-7450
H
Liu
D017360:Arabidopsis / Q000378:metabolism* / Q000502:physiology / Q000528:radiation effects*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D057906:Circadian Clocks / Q000502:physiology* / Q000528:radiation effects*; D002940:Circadian Rhythm / Q000502:physiology / Q000528:radiation effects; D018506:Gene Expression Regulation, Plant / Q000502:physiology / Q000528:radiation effects; D008027:Light; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism*; D013696:Temperature; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism
D029681:Arabidopsis Proteins; C114425:CCA1 protein, Arabidopsis; C000620558:COR27 protein, Arabidopsis; C000620559:COR28 protein, Arabidopsis; C523307:PRR5 protein, Arabidopsis; D012097:Repressor Proteins; C411822:TOC1 protein, Arabidopsis; D014157:Transcription Factors