A SERS nano-tag-based fiber-optic strategy for in situ immunoassay in unprocessed whole blood.
Assay technologies capable of detecting biomarker concentrations in unprocessed whole blood samples are fundamental for applications in medical diagnostics. SERS nano-tags integrated fiber-optic biosensor (FOB) was realized for the first time for in situ immunoassay in whole blood. The reliability and sensitivity of this method rely, in a large extent, on the quality and properties of the SERS nano-tags. The constructed silica-coated Ag SERS nano-tags as labels were used in a rapid and specific in situ FOB immune sensor to detect alpha fetoprotein (AFP) in unprocessed blood samples. Preliminary results of in vivo and in situ dynamic observation of AFP of whole blood in wistar rat highlight the power of this new method.
State Key Laboratory of Luminescence and Applications, Changchun Institute of Optics, Fine Mechanics and Physics, Chinese Academy of Sciences, 130033 Changchun, China; State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, 130033 Changchun, China.
Xiaokun
X
Li
State Key Laboratory of Luminescence and Applications, Changchun Institute of Optics, Fine Mechanics and Physics, Chinese Academy of Sciences, 130033 Changchun, China. Electronic address: zhangyl@ciomp.ac.cn.
Youlin
Y
Zhang
State Key Laboratory of Luminescence and Applications, Changchun Institute of Optics, Fine Mechanics and Physics, Chinese Academy of Sciences, 130033 Changchun, China.
Bin
B
Xue
State Key Laboratory of Luminescence and Applications, Changchun Institute of Optics, Fine Mechanics and Physics, Chinese Academy of Sciences, 130033 Changchun, China. Electronic address: xgkong14@ciomp.ac.cn.
Xianggui
X
Kong
State Key Laboratory of Luminescence and Applications, Changchun Institute of Optics, Fine Mechanics and Physics, Chinese Academy of Sciences, 130033 Changchun, China.
Xiaomin
X
Liu
State Key Laboratory of Luminescence and Applications, Changchun Institute of Optics, Fine Mechanics and Physics, Chinese Academy of Sciences, 130033 Changchun, China.
Langping
L
Tu
State Key Laboratory of Luminescence and Applications, Changchun Institute of Optics, Fine Mechanics and Physics, Chinese Academy of Sciences, 130033 Changchun, China.
Yulei
Y
Chang
Van't Hoff Institute for Molecular Sciences, University of Amsterdam, Science Park 904, 1098 XH Amsterdam, The Netherlands. Electronic address: h.zhang@uva.nl.
Hong
H
Zhang
D000818:Animals; D055910:Antibodies, Immobilized / Q000737:chemistry; D015374:Biosensing Techniques / Q000295:instrumentation*; D004867:Equipment Design; D005336:Fiber Optic Technology / Q000295:instrumentation*; D007118:Immunoassay / Q000295:instrumentation; D053768:Metal Nanoparticles / Q000737:chemistry*; D011817:Rabbits; D017208:Rats, Wistar; D012822:Silicon Dioxide / Q000737:chemistry*; D012834:Silver / Q000737:chemistry*; D013059:Spectrum Analysis, Raman / Q000295:instrumentation*; D000509:alpha-Fetoproteins / Q000032:analysis*
D055910:Antibodies, Immobilized; D000509:alpha-Fetoproteins; D012834:Silver; D012822:Silicon Dioxide
Fiber-optic biosensor; SERS nano-tags; Silica encapsulation; Whole blood