Genetic variants in microRNAs predict non-small cell lung cancer prognosis in Chinese female population in a prospective cohort study.
To investigate the prognostic effect of microRNA single nucleotide polymorphisms (SNP) on non-small cell lung cancer (NSCLC) patients, 658 female participants from northeast China were enrolled in our prospective cohort study and followed up from 2010 to 2015. C-containing genotypes of miR-149 rs2292832 were associated with better overall survival (OS). The joint effect of miR-149 and miR-196a2 and the joint effect of miR-149 and miR-608 were also observed in our study. To verify the function of miR-149 rs2292832, A549 cell lines were stably transfected with lenti-virus containing miR-149-C vector, miR-149-T vector and empty vector. Cells containing C allele assumed a higher expression level of miR-149, a decrease in cell growth and the sensitivity to anticancer drug when compared with cells containing T allele. The role of miR-149 playing in cancer prognosis may function through DNA topoisomerases 1 (TOP1) pathway, according to the results from luciferase reporter assays. In conclusion, miR-149 C allele may be a prognostic biomarker for better NSCLC OS.
Department of Epidemiology, China Medical University, Shenyang, Liaoning, 110122, Peoples R China.|Key Laboratory of Cancer Etiology and Prevention, China Medical University, Liaoning Province Department of Education, Shenyang, Liaoning, 110122, Peoples R China.
Xia
X
Lingzi
Department of Epidemiology, China Medical University, Shenyang, Liaoning, 110122, Peoples R China.|Key Laboratory of Cancer Etiology and Prevention, China Medical University, Liaoning Province Department of Education, Shenyang, Liaoning, 110122, Peoples R China.
Yin
Y
Zhihua
Department of Epidemiology, China Medical University, Shenyang, Liaoning, 110122, Peoples R China.|Key Laboratory of Cancer Etiology and Prevention, China Medical University, Liaoning Province Department of Education, Shenyang, Liaoning, 110122, Peoples R China.
Li
L
Xuelian
Department of Epidemiology, China Medical University, Shenyang, Liaoning, 110122, Peoples R China.|Key Laboratory of Cancer Etiology and Prevention, China Medical University, Liaoning Province Department of Education, Shenyang, Liaoning, 110122, Peoples R China.
Ren
R
Yangwu
Department of Radiotherapy, Shenyang North Hospital, Shenyang, Liaoning, 110001, Peoples R China.
Zhang
Z
Haibo
Department of Radiotherapy Oncology, The Fourth Affiliated Hospital of China Medical University, Shenyang, Liaoning, 110001, Peoples R China.
Zhao
Z
Yuxia
Department of Epidemiology, China Medical University, Shenyang, Liaoning, 110122, Peoples R China.|Key Laboratory of Cancer Etiology and Prevention, China Medical University, Liaoning Province Department of Education, Shenyang, Liaoning, 110122, Peoples R China.
Zhou
Z
Baosen
D000072283:A549 Cells; D000970:Antineoplastic Agents / Q000494:pharmacology; D044466:Asian People / Q000235:genetics; D014408:Biomarkers, Tumor / Q000235:genetics* / Q000378:metabolism; D002289:Carcinoma, Non-Small-Cell Lung / Q000208:ethnology / Q000235:genetics* / Q000378:metabolism / Q000628:therapy; D049109:Cell Proliferation / Q000187:drug effects; D002681:China; D002945:Cisplatin / Q000494:pharmacology; D004264:DNA Topoisomerases, Type I / Q000235:genetics / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D005260:Female; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease; D006801:Humans; D053208:Kaplan-Meier Estimate; D008175:Lung Neoplasms / Q000208:ethnology / Q000235:genetics* / Q000378:metabolism / Q000628:therapy; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D008875:Middle Aged; D010641:Phenotype; D020641:Polymorphism, Single Nucleotide; D011446:Prospective Studies; D012307:Risk Factors; D012737:Sex Factors; D013997:Time Factors; D014162:Transfection
D000970:Antineoplastic Agents; D014408:Biomarkers, Tumor; C551557:MIRN149 microRNA, human; C542543:MIRN196 microRNA, human; C000591804:MIRN608 microRNA, human; D035683:MicroRNAs; D004264:DNA Topoisomerases, Type I; C528909:TOP1 protein, human; D002945:Cisplatin
Chinese; SNP; lung cancer; miRNA; prognosis