Preliminary characterization of Tasmanian aquareovirus (TSRV) isolates.
In an attempt to determine whether or not genetic variants of the Tasmanian strain of Atlantic salmon aquareovirus (TSRV) exist, 14 isolates of TSRV, originating from various locations in Tasmania, covering a 20-year period (1990-2010), obtained from various host species and tissues, and isolated on different cell lines, were selected for this study. Two categories, termed "typical" and "atypical", of variants of TSRV were identified based on preliminary genotypic and phenotypic characterization carried out on these 14 different isolates. In addition, electron microscopic examination indicated the existence of at least three variants based on viral particle size. Finally, this study demonstrated the existence of at least one new variant of TSRV isolates, other than the more commonly isolated typical TSRV isolates, in farmed Tasmanian Atlantic salmon.
National Centre for Marine Conservation and Resource Sustainability, University of Tasmania, Launceston, TAS, 7248, Australia. sandra@umt.edu.my.|School of Fisheries Science and Aquaculture, University Malaysia Terengganu, Terengganu, Malaysia. sandra@umt.edu.my.
Sandra C
SC
Zainathan
Department of Primary Industries, Park, Water and Environment, Launceston, TAS, 7248, Australia.
Jeremy
J
Carson
CSIRO Australian Animal Health Laboratory (AAHL), Geelong, VIC, 3220, Australia.
Mark St J
MS
Crane
CSIRO Australian Animal Health Laboratory (AAHL), Geelong, VIC, 3220, Australia.
Lynette M
LM
Williams
CSIRO Australian Animal Health Laboratory (AAHL), Geelong, VIC, 3220, Australia.
John
J
Hoad
CSIRO Australian Animal Health Laboratory (AAHL), Geelong, VIC, 3220, Australia.
Nicholas J G
NJ
Moody
CSIRO Australian Animal Health Laboratory (AAHL), Geelong, VIC, 3220, Australia.
Nicholas
N
Gudkovs
Andrew
Leis
CSIRO Australian Animal Health Laboratory (AAHL), Geelong, VIC, 3220, Australia.
Sandra
S
Crameri
CSIRO Australian Animal Health Laboratory (AAHL), Geelong, VIC, 3220, Australia.
Alex D
AD
Hyatt
CSIRO Australian Animal Health Laboratory (AAHL), Geelong, VIC, 3220, Australia.
John
J
Young
National Centre for Marine Conservation and Resource Sustainability, University of Tasmania, Launceston, TAS, 7248, Australia.
Barbara F
BF
Nowak
D000818:Animals; D005393:Fish Diseases / Q000821:virology*; D005838:Genotype; D010802:Phylogeny; D012087:Reoviridae / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000648:ultrastructure; D012088:Reoviridae Infections / Q000662:veterinary* / Q000821:virology; D020317:Salmo salar / Q000821:virology; D013648:Tasmania