Papillomavirus infections in the oral and genital mucosa of asymptomatic women.
Human papillomavirus (HPV) has been found in several regions of the body, including the oral cavity. Recently, this virus has been associated with oropharyngeal cancer, but little is known about HPV transmission to the oral cavity. We carried out a study to investigate concurrent oral and cervical infections in 76 asymptomatic women attending a healthcare program. Demographic and behavior data were obtained through a structured questionnaire. Oral and cervical mucosa scrapings were collected and stored for DNA extraction. HPV DNA amplification was performed by polymerase chain reaction assay (PCR) using both primers My09/My11 and FAP59/64, followed by HPV typing with restriction fragment length polymorphism analysis (RFLP) and sequencing. The data collected revealed no risk factors for HPV infection in these 76 women. HPV prevalence of 9.2 and 5.3% was found in cervical and oral mucosa, respectively. Concurrent infections by discordant types were detected in one case only. Sequencing procedures allowed us to detect a new putative HPV 17 subtype from the Betapapillomavirus genus. Our results support the view that cervical and oral HPV infections are independent events. The observed low prevalence of both oral and cervical HPV infections could be associated with attendance in a healthcare program.
Universidade Federal Fluminense (UFF), Departamento de Microbiologia e Parasitologia, Niterói, RJ, Brazil. Electronic address: virologia.uff@gmail.com.
Ledy Horto Santos
LH
Oliveira
Universidade Federal Fluminense (UFF), Departamento de Microbiologia e Parasitologia, Niterói, RJ, Brazil.
Larissa Silva
LS
Santos
Universidade Federal Fluminense (UFF), Departamento de Microbiologia e Parasitologia, Niterói, RJ, Brazil.
Carolina Oliveira
CO
Silva
Universidade Federal Fluminense (UFF), Departamento de Microbiologia e Parasitologia, Niterói, RJ, Brazil.
Everton Faccini
EF
Augusto
Universidade Federal Fluminense (UFF), Departamento de Microbiologia e Parasitologia, Niterói, RJ, Brazil.
Felipe Piedade Gonçalves
FP
Neves
D000293:Adolescent; D000328:Adult; D000368:Aged; D058345:Asymptomatic Infections; D002584:Cervix Uteri / Q000821:virology*; D003430:Cross-Sectional Studies; D004267:DNA Viruses; D005260:Female; D005838:Genotype; D006801:Humans; D008875:Middle Aged; D009059:Mouth Diseases / Q000821:virology*; D009061:Mouth Mucosa / Q000821:virology*; D027383:Papillomaviridae / Q000302:isolation & purification; D030361:Papillomavirus Infections / Q000821:virology*; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D012307:Risk Factors; D011795:Surveys and Questionnaires; D002577:Uterine Cervical Diseases / Q000821:virology*; D055815:Young Adult
HPV; Oral infection; Transmission