Downregulation of miR-181a protects mice from LPS-induced acute lung injury by targeting Bcl-2.
Acute lung injury/acute respiratory distress syndrome (ALI/ARDS) is a progressive syndrome with a high incidence and mortality rate. Apoptosis is a very important pathological feature of ALI. Recently, more and more researches on mircoRNAs are involved in the regulation of proliferation and apoptosis of cancer cells. However, the functions of microRNAs in ALI remain unknown. In this study, PCR array and qRT-PCR were used to investigate microRNA profiles and miR-181a regulates apoptosis induced by LPS. Then, we predict the potential target gene of miR-181a is BCL-2 by bioinformatics methods, and validate it using luciferase reporter gene assays. Finally, the effect of miR-181a on apoptosis was investigated in ALI animal model and cell model induced by LPS. Inhibition expression of miR-181a can significantly reduce LPS-induced acute lung injury in mice and A549 cells. Therefore, we suggest that MiR-181a inhibition protects mice from LPS-induced acute lung injury by targeting Bcl-2.
Burn Center of Yunnan Province, Second Affiliated Hospital of Kunming Medical University, Kunming, China.
Wuquan
W
Li
Department of General Surgery, The 309th Hospital of PLA, Beijing, China.
Xiaochen
X
Qiu
Burn Center of Yunnan Province, Second Affiliated Hospital of Kunming Medical University, Kunming, China.
He
H
Jiang
Burn Center of Yunnan Province, Second Affiliated Hospital of Kunming Medical University, Kunming, China.
Yalong
Y
Han
Burn Center of Yunnan Province, Second Affiliated Hospital of Kunming Medical University, Kunming, China.
Dinan
D
Wei
Burn Center of Yunnan Province, Second Affiliated Hospital of Kunming Medical University, Kunming, China. Electronic address: liujun_63@126.com.
Jun
J
Liu
D000072283:A549 Cells; D055371:Acute Lung Injury / Q000139:chemically induced / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D015536:Down-Regulation / Q000502:physiology*; D018390:Gene Targeting / Q000379:methods*; D008070:Lipopolysaccharides / Q000633:toxicity*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D020011:Protective Agents / Q000378:metabolism; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000235:genetics / Q000378:metabolism*; D011897:Random Allocation
D008070:Lipopolysaccharides; D035683:MicroRNAs; D020011:Protective Agents; D019253:Proto-Oncogene Proteins c-bcl-2; C529100:mirn181 microRNA, mouse
Acute lung injury (ALI); Apoptosis; Bcl-2; Mice; MicroRNA-181a