Increased biofilm formation ability and accelerated transport of Staphylococcus aureus along a catheter during reciprocal movements.
Staphylococcus spp. is a major cause of device-related infections. However, the mechanisms of deep-tissue infection by staphylococci from the skin surface remain unclear. We performed in vitro experiments to determine how staphylococci are transferred from the surface to the deeper layers of agar along the catheter for different strains of Staphylococcus aureus with respect to bacterial concentrations, catheter movements, and biofilm formation. We found that when 5-mm reciprocal movements of the catheter were repeated every 8h, all catheter samples of S. aureus penetrated the typical distance of 50mm from the skin to the epidural space. The number of reciprocal catheter movements and the depth of bacterial growth were correlated. A greater regression coefficient for different strains implied faster bacterial growth. Enhanced biofilm formation by different strains implied larger regression coefficients. Increased biofilm formation ability may accelerate S. aureus transport along a catheter due to physical movements by patients.
Department of Anesthesiology, Chikushi Hospital, Fukuoka University, Japan. Electronic address: haraga@fukuoka-u.ac.jp.
Isao
I
Haraga
Department of Anesthesiology, Fukuoka Higashi Medical Center, Japan.
Shintaro
S
Abe
The Central Laboratory for Pathology and Morphology, Fukuoka University School of Medicine, Japan.
Shiro
S
Jimi
Academia, Industry and Government Collaborative Research Institute of Translational Medicine for Life Innovation, Fukuoka University, Japan.
Fumiaki
F
Kiyomi
Department of Anesthesiology, Fukuoka University Faculty of Medicine, Japan.
Ken
K
Yamaura
D001422:Bacterial Adhesion; D018441:Biofilms / Q000254:growth & development*; D057785:Catheters / Q000382:microbiology*; D006801:Humans; D009068:Movement; D012867:Skin / Q000382:microbiology; D013211:Staphylococcus aureus / Q000302:isolation & purification*
Biofilms; Catheter; Contamination; Infection; Staphylococcus