The General Stress Response σS Is Regulated by a Partner Switch in the Gram-negative Bacterium Shewanella oneidensis.
Here, we show that a partner-switching system of the aquatic Proteobacterium Shewanella oneidensis regulates post-translationally σS(also called RpoS), the general stress response sigma factor. Genes SO2118 and SO2119 encode CrsA and CrsR, respectively. CrsR is a three-domain protein comprising a receiver, a phosphatase, and a kinase/anti-sigma domains, and CrsA is an anti-sigma antagonist. In vitro, CrsR sequesters σSand possesses kinase and phosphatase activities toward CrsA. In turn, dephosphorylated CrsA binds the anti-sigma domain of CrsR to allow the release of σSThis study reveals a novel pathway that post-translationally regulates the general stress response sigma factor differently than what was described for other proteobacteria like Escherichia coli We argue that this pathway allows probably a rapid bacterial adaptation.
From the Aix-Marseille Université, CNRS, BIP UMR7281, 13402 Marseille, France.
Sophie
S
Bouillet
From the Aix-Marseille Université, CNRS, BIP UMR7281, 13402 Marseille, France.
Olivier
O
Genest
From the Aix-Marseille Université, CNRS, BIP UMR7281, 13402 Marseille, France.
Cécile
C
Jourlin-Castelli
From the Aix-Marseille Université, CNRS, BIP UMR7281, 13402 Marseille, France.
Michel
M
Fons
From the Aix-Marseille Université, CNRS, BIP UMR7281, 13402 Marseille, France.
Vincent
V
Méjean
From the Aix-Marseille Université, CNRS, BIP UMR7281, 13402 Marseille, France iobbi@imm.cnrs.fr.
Chantal
C
Iobbi-Nivol
D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D010749:Phosphoprotein Phosphatases / Q000235:genetics / Q000378:metabolism*; D010766:Phosphorylation / Q000502:physiology; D011494:Protein Kinases / Q000235:genetics / Q000378:metabolism*; D020592:Shewanella / Q000235:genetics / Q000378:metabolism*; D012808:Sigma Factor / Q000235:genetics / Q000378:metabolism*; D013312:Stress, Physiological / Q000502:physiology*
D001426:Bacterial Proteins; D012808:Sigma Factor; C069567:sigma factor KatF protein, Bacteria; D011494:Protein Kinases; D010749:Phosphoprotein Phosphatases
bacteria; bacterial protein kinase; bacterial protein phosphatase; protein-protein interaction; stress response