Preparation of Co3O4/crumpled graphene microsphere as peroxidase mimetic for colorimetric assay of ascorbic acid.
The well-dispersed Co3O4nanoparticles-decorated crumpled graphene microsphere (CGM) was successfully prepared by aerosol-assisted frying self-assembly and annealing process. It is found that the obtained Co3O4/CGM nanohybrid possessed enhanced intrinsic peroxidase-like activity and could catalytically oxidize 3,3',5,5'-tetramethylbenzidine by H2O2to produce a typical blue product. But the presence of ascorbic acid could induce the reduction of oxTMB to TMB, resulting in a significant blue color fading. Therefore, a simple, sensitive and selective colorimetric method to detect ascorbic acid was established with a good linear relationship (30-140μM) and a low detection limit of 0.19μM. Meanwhile, the selectivity, stability and repeatability were acceptable. It is also a facile route to fabricate nanoparticles/CGM as high-performance enzyme mimetic for colorimetric biosensing.
Department of Materials Science and Engineering, Ocean University of China, 266100 Qingdao, PR China.
Sisi
S
Fan
Department of Materials Science and Engineering, Ocean University of China, 266100 Qingdao, PR China. Electronic address: zhaomg@ouc.edu.cn.
Minggang
M
Zhao
Department of Materials Science and Engineering, Ocean University of China, 266100 Qingdao, PR China.
Longjiang
L
Ding
Optoelectronic Materials and Technologies Engineering Laboratory of Shandong, Physics Department, Qingdao University of Science and Technology, Qingdao 266100, PR China.
Hui
H
Li
Department of Materials Science and Engineering, Ocean University of China, 266100 Qingdao, PR China. Electronic address: sgchen@ouc.edu.cn.
Shougang
S
Chen
D001205:Ascorbic Acid / Q000032:analysis*; D001560:Benzidines / Q000737:chemistry; D040761:Biomimetic Materials / Q000737:chemistry*; D015374:Biosensing Techniques / Q000379:methods; D002384:Catalysis; D002863:Chromogenic Compounds / Q000737:chemistry; D003035:Cobalt / Q000737:chemistry*; D003124:Colorimetry / Q000379:methods*; D006108:Graphite / Q000737:chemistry*; D006861:Hydrogen Peroxide / Q000737:chemistry; D057230:Limit of Detection; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D010084:Oxidation-Reduction; D010087:Oxides / Q000737:chemistry*; D009195:Peroxidase / Q000737:chemistry*
D001560:Benzidines; D002863:Chromogenic Compounds; D010087:Oxides; C021758:3,3',5,5'-tetramethylbenzidine; D003035:Cobalt; D006108:Graphite; D006861:Hydrogen Peroxide; D009195:Peroxidase; D001205:Ascorbic Acid; C060728:cobalt oxide
Ascorbic acid detection; Co(3)O(4); Colorimetric; Crumpled graphene; Peroxidase mimetic