Protein aggregation under high concentration/density state during chromatographic and ultrafiltration processes.
Local transient high protein concentration or high density condition can occur during processing of protein solutions. Typical examples are saturated binding of proteins during column chromatography and high protein concentration on the semi-permeable membrane during ultrafiltration. Both column chromatography and ultrafiltration are fundamental technologies, specially for production of pharmaceutical proteins. We summarize here our experiences related to such high concentration conditions.
Alliance Protein Laboratories, 6042 Cornerstone Court West, San Diego, CA 92121, United States. Electronic address: tarakawa@ap-lab.com.
Tsutomu
T
Arakawa
AminoScience Laboratories, Ajinomoto Inc., 1-1 Kawasaki, Japan.
Daisuke
D
Ejima
Product Development Division, Kyokuto Pharmaceutical Industrial Co., Ltd., 3333-26, Aza-Asayama, Kamitezuna Takahagi-shi, Ibaraki, 318-0004, Japan.
Teruo
T
Akuta
D001120:Arginine / Q000737:chemistry*; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D008567:Membranes, Artificial; D066329:Protein Aggregates; D011485:Protein Binding; D011506:Proteins / Q000737:chemistry*; D012996:Solutions; D014462:Ultrafiltration
D008567:Membranes, Artificial; D066329:Protein Aggregates; D011506:Proteins; D012996:Solutions; D001120:Arginine
Aggregation; Chromatography; High concentration; High density; Ultrafiltration