Genome-Wide Identification, Characterization, and Stress-Responsive Expression Profiling of Genes Encoding LEA (Late Embryogenesis Abundant) Proteins in Moso Bamboo (Phyllostachys edulis).
Late embryogenesis abundant (LEA) proteins have been identified in a wide range of organisms and are believed to play a role in the adaptation of plants to stress conditions. In this study, we performed genome-wide identification of LEA proteins and their coding genes in Moso bamboo (Phyllostachys edulis) of Poaceae. A total of 23 genes encoding LEA proteins (PeLEAs) were found in P. edulis that could be classified to six groups based on Pfam protein family and homologous analysis. Further in silico analyses of the structures, gene amount, and biochemical characteristics were conducted and compared with those of O. sativa (OsLEAs), B. distachyon (BdLEAs), Z. mays (ZmLEAs), S. bicolor (SbLEAs), Arabidopsis, and Populus trichocarpa. The less number of PeLEAs was found. Evolutionary analysis revealed orthologous relationship and colinearity between P. edulis, O. sativa, B. distachyon, Z. mays, and S. bicolor. Analyses of the non-synonymous (Ka) and synonymous (Ks)substitution rates and their ratios indicated that the duplication of PeLEAs may have occurred around 18.8 million years ago (MYA), and divergence time of LEA family among the P. edulis-O. sativa and P. edulis-B. distachyon, P. edulis-S. bicolor, and P. edulis-Z. mays was approximately 30 MYA, 36 MYA, 48 MYA, and 53 MYA, respectively. Almost all PeLEAs contain ABA- and (or) stress-responsive regulatory elements. Further RNA-seq analysis revealed approximately 78% of PeLEAs could be up-regulated by dehydration and cold stresses. The present study makes insights into the LEA family in P. edulis and provides inventory of stress-responsive genes for further functional validation and transgenic research aiming to plant genetic improvement of abiotic stress tolerance.
College of Landscape Architecture, Sichuan Agricultural University, 211 Huimin Road, Wenjiang, Chengdu 611130, Sichuan, China.
Zhuo
Z
Huang
College of Landscape Architecture, Sichuan Agricultural University, 211 Huimin Road, Wenjiang, Chengdu 611130, Sichuan, China.
Xiao-Juan
XJ
Zhong
College of Landscape Architecture, Sichuan Agricultural University, 211 Huimin Road, Wenjiang, Chengdu 611130, Sichuan, China.
Jiao
J
He
College of Landscape Architecture, Sichuan Agricultural University, 211 Huimin Road, Wenjiang, Chengdu 611130, Sichuan, China.
Si-Han
SH
Jin
College of Landscape Architecture, Sichuan Agricultural University, 211 Huimin Road, Wenjiang, Chengdu 611130, Sichuan, China.
Han-Du
HD
Guo
College of Landscape Architecture, Sichuan Agricultural University, 211 Huimin Road, Wenjiang, Chengdu 611130, Sichuan, China.
Xiao-Fang
XF
Yu
College of Landscape Architecture, Sichuan Agricultural University, 211 Huimin Road, Wenjiang, Chengdu 611130, Sichuan, China.
Yu-Jue
YJ
Zhou
College of Landscape Architecture, Sichuan Agricultural University, 211 Huimin Road, Wenjiang, Chengdu 611130, Sichuan, China.
Xi
X
Li
College of Landscape Architecture, Sichuan Agricultural University, 211 Huimin Road, Wenjiang, Chengdu 611130, Sichuan, China.
Ming-Dong
MD
Ma
College of Landscape Architecture, Sichuan Agricultural University, 211 Huimin Road, Wenjiang, Chengdu 611130, Sichuan, China.
Qi-Bing
QB
Chen
Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, Sichuan, China.
Hai
H
Long
D000222:Adaptation, Physiological / Q000235:genetics; D002874:Chromosome Mapping; D032461:Chromosomes, Plant; D003080:Cold Temperature; D055864:Droughts; D019143:Evolution, Molecular; D020869:Gene Expression Profiling / Q000379:methods*; D018506:Gene Expression Regulation, Plant; D014644:Genetic Variation; D018745:Genome, Plant / Q000235:genetics*; D005810:Multigene Family; D012275:Oryza / Q000235:genetics; D010940:Plant Proteins / Q000145:classification / Q000235:genetics*; D006109:Poaceae / Q000145:classification / Q000235:genetics*; D013312:Stress, Physiological; D026801:Synteny; D013997:Time Factors
D010940:Plant Proteins; C066246:late embryogenesis abundant protein, plant