Influence of chitosan nanoparticle-mediated C-erbB-2 gene silencing on invasion and apoptosis of Hep-2 cells.
We aimed to measure the invasion ability of Hep-2 laryngeal cancer cells after treatment with C-erbB-2-small interfering RNA (siRNA)-chitosan nanoparticles, and assess the applied value of chitosan nanoparticle-mediated C-erbB-2 interference in inhibiting laryngeal cancer invasion and metastasis. Nanoparticles of approximately 100 nm, comprising C-erbB-2 siRNA packaged with chitosan, were prepared and used to treat Hep-2 cells. Silencing of C-erbB-2 was detected by western blot and polymerase chain reaction. Cell invasion and apoptosis were estimated by transwell assay and flow cytometry, respectively. C-erbB-2-siRNA-chitosan nanoparticles significantly down-regulated C-erbB-2 expression in Hep-2 cells (P < 0.05), and cell invasion was noticeably decreased. Moreover, they significantly induced apoptosis of the Hep-2 cells (P < 0.05). Chitosan nanoparticle-mediated C-erbB-2 gene interference can inhibit the invasion of laryngeal cancer cells and induce their apoptosis.
Teaching and Research Division of Pathology, Medical College of Yangtze University, Jingzhou, Hubei Province, China.
W R
WR
Liu
Teaching and Research Division of Surgical Medicine, Hubei College of Chinese Medicine, Jingzhou, China.
L R
LR
Cao
Department of Ophthalmology, First Hospital of Jingzhou, Jingzhou, Hubei Province, China zuoguojindo@yeah.net.
G J
GJ
Zuo
D017209:Apoptosis; D015153:Blotting, Western; D045744:Cell Line, Tumor; D048271:Chitosan / Q000737:chemistry*; D005434:Flow Cytometry; D020868:Gene Silencing; D018734:Genes, erbB-2; D006801:Humans; D007822:Laryngeal Neoplasms / Q000473:pathology; D018625:Microscopy, Atomic Force; D053758:Nanoparticles / Q000737:chemistry*; D009361:Neoplasm Invasiveness; D010316:Particle Size; D034741:RNA, Small Interfering / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction
D034741:RNA, Small Interfering; D048271:Chitosan