Simultaneous quantification of Myelin Basic Protein and Tau proteins in cerebrospinal fluid and serum of Multiple Sclerosis patients using nanoimmunosensor.
This study was aimed at the development of an immunosensor for the simultaneous quantification of Myelin Basic Protein (MBP) and Tau proteins in cerebrospinal fluid (CSF) and serum, obtained from Multiple Sclerosis (MS) patients. The newly developed GO/pPG/anti-MBP/anti-Tau nanoimmunosensor has been established by immobilization of MBP and Tau antibodies. The newly developed nanoimmunosensor was tested, optimized and characterized using differential pulse voltammetry (DPV) and electrochemical impedance spectroscopy (EIS). The developed nanoimmunosensor was seen to have detection limits of 0.30nM for MBP and 0.15nM for Tau proteins which were sufficient for the levels to be analysed in neuro-clinic. The clinical study performed using CSF and serum of MS patients showed that the designed nanoimmunosensor was capable of detecting the proteins properly, that were essentially proven by ELISA.
Department of Chemistry, Ankara University, Tandogan, Ankara 06100, Turkey.
Burak
B
Derkus
Department of Chemistry, Ankara University, Tandogan, Ankara 06100, Turkey.
Pinar
P
Acar Bozkurt
Department of Chemistry, Yildiz Technical University, Esenler, Istanbul 34220, Turkey.
Metin
M
Tulu
Department of Chemistry, Ankara University, Tandogan, Ankara 06100, Turkey.
Kaan C
KC
Emregul
Department of Neurology, Medical School, Ankara University, Ibni Sina Hospital, Sihhiye, Ankara 06100, Turkey.
Canan
C
Yucesan
Department of Chemistry, Ankara University, Tandogan, Ankara 06100, Turkey. Electronic address: eemregul@yahoo.com.
Emel
E
Emregul
D055910:Antibodies, Immobilized / Q000737:chemistry; D015374:Biosensing Techniques / Q000379:methods*; D019187:Cadmium Compounds / Q000737:chemistry; D050091:Dendrimers / Q000737:chemistry; D058266:Dielectric Spectroscopy / Q000379:methods; D055664:Electrochemical Techniques / Q000379:methods; D006108:Graphite / Q000737:chemistry; D006801:Humans; D007118:Immunoassay / Q000379:methods; D007854:Lead / Q000737:chemistry; D057230:Limit of Detection; D009103:Multiple Sclerosis / Q000097:blood* / Q000134:cerebrospinal fluid*; D004676:Myelin Basic Protein / Q000097:blood* / Q000134:cerebrospinal fluid*; D053758:Nanoparticles / Q000737:chemistry / Q000648:ultrastructure; D010087:Oxides / Q000737:chemistry; D013440:Sulfides / Q000737:chemistry; D016875:tau Proteins / Q000097:blood* / Q000134:cerebrospinal fluid*
D055910:Antibodies, Immobilized; D019187:Cadmium Compounds; D050091:Dendrimers; D004676:Myelin Basic Protein; D010087:Oxides; D013440:Sulfides; D016875:tau Proteins; C034939:cadmium sulfide; C018391:lead sulfide; D007854:Lead; D006108:Graphite
Dendrimer; Differential pulse voltammetry; Electrochemical impedance spectroscopy; Graphene oxide; Immunosensor; Multiple Sclerosis; Myelin Basic Protein; Tau