MicroRNA-146a Contributes to SCI Recovery via RegulatingTRAF6andIRAK1Expression.
MicroRNA-146a participates in spinal cord injury (SCI) recovery. Until recently, how miRNA-146a participates in SCI remained unclear. In this study, we tried to explore the roles of miRNA-146a in the recovery of SCI using a rat model. The expression of the probable target genes of miRNA-146a (including IRAK1 and TARF6) as well as proinflammation cytokines were measured until 7 days after surgery in the three groups (sham group, SCI group, and miRNA-146a antagomir injection group). Also, the animals' motivations were estimated using Basso Beattie Bresnahan (BBB) during the whole experiment. A luciferase assay was performed to demonstrate that miRNA-146a could directly target the mRNAs ofIRAK1andTRAF6. Our experiments indicate that miRNA-146a inhibits proinflammatory cytokine secretion by suppressingIRAK1andTRAF6expression in the SCI model. In contrast, miRNA-146a may be upregulated by inflammatory mediators via theIRAK1/TRAF6pathway in the spinal cord. As a negative feedback element, miRNA-146a could make sure that the expression ofIRAK1- andTRAF6-mediated genes was under tight control. Thus, miRNA-146a may serve as a novel therapeutic target for SCI interventions.
Department of Orthopedics, The First Affiliated Hospital of Jinan University, Guangzhou, China.
Jinsong
J
Wei
Stem Cell Research and Cellular Therapy Center, Affiliated Hospital of Guangdong Medical University, Zhanjiang, China.
Jiafeng
0000-0002-2995-8349
J
Wang
Stem Cell Research and Cellular Therapy Center, Affiliated Hospital of Guangdong Medical University, Zhanjiang, China.
Yulan
Y
Zhou
Stem Cell Research and Cellular Therapy Center, Affiliated Hospital of Guangdong Medical University, Zhanjiang, China.
Shouquan
S
Yan
Institute of Neurology, Affiliated Hospital of Guangdong Medical University, Zhanjiang, China; Stroke Center, Neurology & Neurosurgery Division, The Clinical Medicine Research Institute and The First Affiliated Hospital of Jinan University, Guangzhou, China.
Keshen
0000-0003-2389-7706
K
Li
Department of Orthopedics, The First Affiliated Hospital of Jinan University, Guangzhou, China.
Hongsheng
0000-0002-6945-8875
H
Lin
D000818:Animals; D005260:Female; D005786:Gene Expression Regulation; D053592:Interleukin-1 Receptor-Associated Kinases / Q000378:metabolism*; D035683:MicroRNAs / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D020127:Recovery of Function; D013119:Spinal Cord Injuries / Q000503:physiopathology*; D048029:TNF Receptor-Associated Factor 6 / Q000378:metabolism*; D013904:Thoracic Vertebrae / Q000293:injuries
C554180:MIRN146 microRNA, rat; D035683:MicroRNAs; D048029:TNF Receptor-Associated Factor 6; C547807:IRAK1 protein, rat; D053592:Interleukin-1 Receptor-Associated Kinases