Polymorphism in Murine mtATP8 Gene Correlates with Decreased Reactive Oxygen Species in Aging Hematopoietic Cells.
Mitochondrial DNA (mtDNA) encodes for the respiratory chain proteins. Genetic alterations in mtDNA have been described during aging and linked to impaired hematopoiesis.



We investigated two novel conplastic mouse strains harboring a mitochondrial nt7778 G/T polymorphism leading to an amino acid exchange in respiratory chain complex V. Effects on reactive oxygen species (ROS) and adenosine triphosphate (ATP) levels, as well as bone marrow composition and peripheral blood counts, were investigated during aging (up to 24 month).



The polymorphism correlated with significantly decreased ROS levels in aged mice. Effects on hematopoiesis were marginal and not statistically significant: numbers of erythroid cells in bone marrow, as well as mean corpuscular hemoglobin, tended to decrease over time.



The investigated polymorphism is associated with decreased ROS levels in aged hematopoietic cells but does not significantly influence hematopoiesis itself.
Department of Medicine III, Hematology/Oncology/Palliative Medicine, Rostock University Medical Center, Rostock, Germany.
Catrin
C
Roolf
Department of Medicine III, Hematology/Oncology/Palliative Medicine, Rostock University Medical Center, Rostock, Germany.
Christin
C
Kretzschmar
Department of Medicine III, Hematology/Oncology/Palliative Medicine, Rostock University Medical Center, Rostock, Germany.
Katrin
K
Timmer
Department of Medicine III, Hematology/Oncology/Palliative Medicine, Rostock University Medical Center, Rostock, Germany.
Anett
A
Sekora
Department of Medicine III, Hematology/Oncology/Palliative Medicine, Rostock University Medical Center, Rostock, Germany.
Gudrun
G
Knübel
Department of Medicine III, Hematology/Oncology/Palliative Medicine, Rostock University Medical Center, Rostock, Germany.
Hugo
H
Murua Escobar
Institute of Biostatistics and Informatics in Medicine and Ageing Research, Rostock University Medical Center, Rostock, Germany.
Georg
G
Fuellen
Institute of Experimental Dermatology, University of Lübeck, Lübeck, Germany.
Saleh M
SM
Ibrahim
Institute of Medical Biochemistry and Molecular Biology, Rostock University Medical Center, Rostock, Germany.
Markus
M
Tiedge
Institute of Medical Biochemistry and Molecular Biology, Rostock University Medical Center, Rostock, Germany.
Simone
S
Baltrusch
Department of Medicine II, Division of Gastroenterology, Rostock University Medical Center, Rostock, Germany.
Sarah
S
Müller
Oscar Langendorff Institute of Physiology, Rostock University Medical Center, Rostock, Germany.
Rüdiger
R
Köhling
Department of Medicine III, Hematology/Oncology/Palliative Medicine, Rostock University Medical Center, Rostock, Germany Christian.Junghanss@med.uni-rostock.de.
Christian
C
Junghanss
D000255:Adenosine Triphosphate / Q000378:metabolism; D000375:Aging / Q000235:genetics; D000818:Animals; D002478:Cells, Cultured; D016922:Cellular Senescence / Q000235:genetics*; D004272:DNA, Mitochondrial / Q000235:genetics*; D041905:Erythroid Cells / Q000166:cytology / Q000378:metabolism; D006410:Hematopoiesis / Q000235:genetics; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000378:metabolism*; D006454:Hemoglobins / Q000378:metabolism; D008806:Mice, Inbred AKR; D008810:Mice, Inbred C57BL; D008815:Mice, Inbred Strains; D025261:Mitochondrial Proton-Translocating ATPases / Q000235:genetics* / Q000378:metabolism; D020641:Polymorphism, Single Nucleotide; D017382:Reactive Oxygen Species / Q000378:metabolism*; D013045:Species Specificity
D004272:DNA, Mitochondrial; D006454:Hemoglobins; D017382:Reactive Oxygen Species; D000255:Adenosine Triphosphate; D025261:Mitochondrial Proton-Translocating ATPases; C546989:mt-Atp8 protein, mouse
ROS; conplastic mice; hematopoiesis; mtDNA; stem cells