RNAe in a transgenic growth hormone mouse model shows potential for use in gene therapy.
OBJECTIVE
RNAe is a new method that enhances protein expression at the post-transcriptional level. RNAe utility was further explored to improve endogenous protein expression.


RESULTS
Transgenic mice were created by targeting RNAe to growth hormone gene into the C57/BL mouse genome by transposon mediated integration; the mice showed a heavier body weight and longer body length compared with normal mice. RNAe can also be used for gene therapy through the delivery of in vitro transcribed RNA.


CONCLUSIONS
This study takes a further step towards applying RNAe in pharmaceutical approaches by transposon-based transgenic mice model construction and the use of in vitro transcribed RNA transfection assay.
MOE Key Laboratory of Bioinformatics, School of Life Sciences, Tsinghua University, Beijing, 100084, China.
Haizhou
H
Long
MOE Key Laboratory of Bioinformatics, School of Life Sciences, Tsinghua University, Beijing, 100084, China.
Yi
Y
Yao
MOE Key Laboratory of Bioinformatics, School of Life Sciences, Tsinghua University, Beijing, 100084, China.
Shouhong
S
Jin
MOE Key Laboratory of Bioinformatics, School of Life Sciences, Tsinghua University, Beijing, 100084, China.
Yingting
Y
Yu
ViewSolid Biotech, Beijing, 100085, China.
Xiongbing
X
Hu
ViewSolid Biotech, Beijing, 100085, China.
Fengfeng
F
Zhuang
GenoArray Biotech, Suzhou, 215123, China.
Hanshuo
H
Zhang
MOE Key Laboratory of Bioinformatics, School of Life Sciences, Tsinghua University, Beijing, 100084, China. wuqiong@mail.tsinghua.edu.cn.
Qiong
Q
Wu
D000818:Animals; D002460:Cell Line; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005434:Flow Cytometry; D015316:Genetic Therapy / Q000379:methods; D013006:Growth Hormone / Q000235:genetics*; D006367:HeLa Cells; D006801:Humans; D008297:Male; D051379:Mice; D008822:Mice, Transgenic; D011247:Pregnancy; D062085:RNA, Long Noncoding / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics*
D062085:RNA, Long Noncoding; D012333:RNA, Messenger; D013006:Growth Hormone
Growth hormone; Pharmaceutical application; Protein production; RNAe; Transgenic mice