Organic amendments enhance Pb tolerance and accumulation during micropropagation of Daphne jasminea.
The study investigated the effects of organic amendments: pineapple pulp (PP) and agar hydrolyzate (AH), on micropropagation and Pb bioaccumulation and tolerance in a woody shrub Daphne jasminea cultured in vitro. The amendments were analyzed for their content of carbohydrates, phenolic acids, and phytohormones and added at a dose of 10 mL L-1to the medium containing 1.0 mM lead nitrate. Micropropagation coefficient increased by 10.2-16.6 % in PP and AH variants, respectively. Growth tolerance index increased by 22.9-31.8 % for the shoots and by 60.1-82.4 % for the roots. In the absence of Pb, the additives inhibited multiplication and growth of microplantlets. PP and AH facilitated Pb accumulation in plant organs, especially in the roots. PP enhanced bioconcentration factor and AH improved Pb translocation to the shoots. Adaptation to Pb was associated with increased accumulation of phenolics and higher radical scavenging activity. Medium supplementation, particularly with AH, enhanced antiradical activity of Pb-adapted lines but reduced the content of phenolic compounds. The study results indicated that supplementation with organic amendments may be beneficial in in vitro selection against lead toxicity.
Unit of Botany and Plant Physiology, Institute of Plant Biology and Biotechnology, Faculty of Biotechnology and Horticulture, University of Agriculture in Kraków, Al. 29 Listopada 54, 31-425, Kraków, Poland. a.wiszniewska@ogr.ur.krakow.pl.
Alina
A
Wiszniewska
Department of Botany, Faculty of Agriculture and Biology, Warsaw University of Life Sciences (SGGW), Nowoursynowska 159, Building 37, 02-776, Warszawa, Poland.
Ewa
E
Muszyńska
Unit of Botany and Plant Physiology, Institute of Plant Biology and Biotechnology, Faculty of Biotechnology and Horticulture, University of Agriculture in Kraków, Al. 29 Listopada 54, 31-425, Kraków, Poland.
Ewa
E
Hanus-Fajerska
Unit of Plant Nutrition, Institute of Plant Biology and Biotechnology, Faculty of Biotechnology and Horticulture, University of Agriculture in Kraków, Al. 29 Listopada 54, 31-425, Kraków, Poland.
Sylwester
S
Smoleń
The Franciszek Górski Institute of Plant Physiology, Polish Academy of Sciences, Niezapominajek 21, 30-239, Kraków, Poland.
Michał
M
Dziurka
The Franciszek Górski Institute of Plant Physiology, Polish Academy of Sciences, Niezapominajek 21, 30-239, Kraków, Poland.
Kinga
K
Dziurka
D032364:Daphne / Q000187:drug effects*; D007854:Lead / Q000633:toxicity*; D009566:Nitrates / Q000633:toxicity*; D018517:Plant Roots / Q000187:drug effects; D012987:Soil / Q000737:chemistry*; D012989:Soil Pollutants / Q000032:analysis / Q000633:toxicity*
D009566:Nitrates; D012987:Soil; D012989:Soil Pollutants; D007854:Lead; C017461:lead nitrate
Antioxidant activity; Biostimulation; In vitro culture; Lead adaptation; Medium supplements; Phenolic compounds; Thymelaeaceae