Enhanced osteogenesis of β-tricalcium phosphate reinforced silk fibroin scaffold for bone tissue biofabrication.
Scaffolds, used for tissue regeneration are important to preserve their function and morphology during tissue healing. Especially, scaffolds for bone tissue engineering should have high mechanical properties to endure load of bone. Silk fibroin (SF) from Bombyx mori silk cocoon has potency as a type of biomaterials in the tissue engineering. β-tricalcium phosphate (β-TCP) as a type of bioceramics is also critical as biomaterials for bone regeneration because of its biocompatibility, osteoconductivity, and mechanical strength. The aim of this study was to fabricate three-dimensional SF/β-TCP scaffolds and access its availability for bone grafts through in vitro and in vivo test. The scaffolds were fabricated in each different ratios of SF and β-TCP (100:0, 75:25, 50:50, 25:75). The characterizations of scaffolds were conducted by FT-IR, compressive strength, porosity, and SEM. The in vitro and in vivo tests were carried out by MTT, ALP, RT-PCR, SEM, μ-CT, and histological staining. We found that the SF/β-TCP scaffolds have high mechanical strength and appropriate porosity for bone tissue engineering. The study showed that SF/β-TCP (75:25) scaffold exhibited the highest osteogenesis compared with other scaffolds. The results suggested that SF/β-TCP (75:25) scaffold can be applied as one of potential bone grafts for bone tissue engineering.
Department of BIN Convergence Technology, Department of Polymer. Nano Science & Technology and Polymer Fusion Research Center, Chonbuk National University, 567 Baekje-daero, Deokjin-gu, Jeonju, Jeollabuk-do, 54896, Republic of Korea.
Dae Hoon
DH
Lee
Department of BIN Convergence Technology, Department of Polymer. Nano Science & Technology and Polymer Fusion Research Center, Chonbuk National University, 567 Baekje-daero, Deokjin-gu, Jeonju, Jeollabuk-do, 54896, Republic of Korea.
Nirmalya
N
Tripathy
Department of BIN Convergence Technology, Department of Polymer. Nano Science & Technology and Polymer Fusion Research Center, Chonbuk National University, 567 Baekje-daero, Deokjin-gu, Jeonju, Jeollabuk-do, 54896, Republic of Korea.
Jae Hun
JH
Shin
Department of BIN Convergence Technology, Department of Polymer. Nano Science & Technology and Polymer Fusion Research Center, Chonbuk National University, 567 Baekje-daero, Deokjin-gu, Jeonju, Jeollabuk-do, 54896, Republic of Korea.
Jeong Eun
JE
Song
Department of BIN Convergence Technology, Department of Polymer. Nano Science & Technology and Polymer Fusion Research Center, Chonbuk National University, 567 Baekje-daero, Deokjin-gu, Jeonju, Jeollabuk-do, 54896, Republic of Korea.
Jae Geun
JG
Cha
Research and Development Institute, CGbio Corporation, Seongnam, Korea.
Kyung Dan
KD
Min
Department of Otorhinolaryngology-Head and Neck Surgery, Chuncheon Sacred Heart Hospital, College of Medicine, Hallym University, 1-1 Okcheon, Chuncheon, Gangwon 200-702, Republic of Korea.
Chan Hum
CH
Park
Department of BIN Convergence Technology, Department of Polymer. Nano Science & Technology and Polymer Fusion Research Center, Chonbuk National University, 567 Baekje-daero, Deokjin-gu, Jeonju, Jeollabuk-do, 54896, Republic of Korea. Electronic address: gskhang@jbnu.ac.kr.
Gilson
G
Khang
D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry / Q000494:pharmacology; D001696:Biomechanical Phenomena / Q000187:drug effects; D001842:Bone and Bones / Q000166:cytology / Q000187:drug effects* / Q000502:physiology*; D002113:Calcification, Physiologic / Q000187:drug effects; D002130:Calcium Phosphates / Q000737:chemistry*; D002454:Cell Differentiation / Q000187:drug effects; D005260:Female; D005349:Fibroins / Q000737:chemistry* / Q000494:pharmacology*; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000187:drug effects; D010012:Osteogenesis / Q000187:drug effects*; D011817:Rabbits; D051381:Rats; D023822:Tissue Engineering; D054457:Tissue Scaffolds / Q000737:chemistry*
D001672:Biocompatible Materials; D002130:Calcium Phosphates; C485817:beta-tricalcium phosphate; D005349:Fibroins; D000469:Alkaline Phosphatase
Bone marrow-derived mesenchymal stem cells; Osteogenic differentiation; Scaffolds; Silk fibroin; β-Tricalcium Phosphate