Identification of novel and differentially expressed MicroRNAs in goat enzootic nasal adenocarcinoma.
MicroRNAs (miRNAs) post-transcriptionally regulate a variety of genes involved in eukaryotic cell growth, development, metabolism and other biological processes, and numerous miRNAs are implicated in the initiation and progression of cancer. Enzootic nasal adenocarcinoma (ENA), an epithelial tumor induced in goats and sheep by enzootic nasal tumor virus (ENTV), is a chronic, progressive, contact transmitted disease.



In this work, small RNA Illumina high-throughput sequencing was used to construct a goat nasal miRNA library. This study aimed to identify novel and differentially expressed miRNAs in the tumor and para-carcinoma nasal tissues of Nanjiang yellow goats with ENA.



Four hundred six known miRNAs and 29 novel miRNAs were identified. A total of 116 miRNAs were significantly differentially expressed in para-carcinoma nasal tissues and ENA (54 downregulated; 60 upregulated; two only expressed in control group); Target gene prediction and functional analysis revealed that 6176 non-redundancy target genes, 1792 significant GO and 97 significant KEGG pathway for 121 miRNAs (116 significant expression miRNAs and five star sequence) were predicted. GO and KEGG pathway analysis revealed the majority of target genes in ENA are involved in cell proliferation, signal transduction and other processes associated with cancer.



This is the first large-scale identification of miRNAs in Capra hircus ENA and provides a theoretical basis for investigating the complicated miRNA-mediated regulatory networks involved in the pathogenesis and progression of ENA.
College of Veterinary Medicine, Sichuan Agricultural University, Hui Min Road 211, Chengdu, Sichuan, 611130, People's Republic of China.
Bin
B
Wang
College of Veterinary Medicine, Sichuan Agricultural University, Hui Min Road 211, Chengdu, Sichuan, 611130, People's Republic of China.
Ni
N
Ye
College of Veterinary Medicine, Sichuan Agricultural University, Hui Min Road 211, Chengdu, Sichuan, 611130, People's Republic of China.
San-Jie
SJ
Cao
College of Veterinary Medicine, Sichuan Agricultural University, Hui Min Road 211, Chengdu, Sichuan, 611130, People's Republic of China.
Xin-Tian
XT
Wen
College of Veterinary Medicine, Sichuan Agricultural University, Hui Min Road 211, Chengdu, Sichuan, 611130, People's Republic of China.
Yong
Y
Huang
College of Veterinary Medicine, Sichuan Agricultural University, Hui Min Road 211, Chengdu, Sichuan, 611130, People's Republic of China. yanqigui@126.com.
Qi-Gui
QG
Yan
D000230:Adenocarcinoma / Q000662:veterinary*; D000820:Animal Diseases / Q000235:genetics* / Q000473:pathology; D000818:Animals; D019295:Computational Biology / Q000379:methods; D030561:Databases, Nucleic Acid; D015972:Gene Expression Regulation, Neoplastic; D015723:Gene Library; D063990:Gene Ontology; D053263:Gene Regulatory Networks; D006041:Goats / Q000235:genetics*; D035683:MicroRNAs / Q000235:genetics*; D058977:Molecular Sequence Annotation; D009669:Nose Neoplasms / Q000662:veterinary*; D034622:RNA Interference; D012333:RNA, Messenger / Q000235:genetics
D035683:MicroRNAs; D012333:RNA, Messenger
Enzootic nasal adenocarcinoma; Gene ontology; Illumina high-throughput sequencing; KEGG pathway; MicroRNA