Screening and characterization of novel microsatellite loci in Lateolabrax japonicus.
Lateolabrax japonicus, an economically important species, is widely consumed in the offshore coasts of China, Korea, and Japan. We identified 10 new L. japonicus microsatellite markers, using a modified protocol of fast isolation by AFLP of sequences containing repeats. Thirty L. japonicus individuals were collected from Xiamen, China, to evaluate the degree of polymorphism. The number of identified alleles ranged from three to five. The polymorphism information content varied from 0.267 to 0.711, whereas the observed and expected heterozygosities ranged from 0.249 to 0.706 and 0.294 to 0.751, respectively. One of the 10 loci (L10) deviated from the Hardy-Weinberg equilibrium. These new microsatellite markers will provide a useful tool for the determination of population genetic structure and genetic diversity in L. japonicus.
Fisheries College, Jimei University, Xiamen, China.|Fujian Provincial Key Laboratory of Marine Fishery Resources and Eco-Environment, Xiamen, China.
B B
BB
Li
Fisheries College, Jimei University, Xiamen, China lizhongbao@jmu.edu.cn.|Fujian Provincial Key Laboratory of Marine Fishery Resources and Eco-Environment, Xiamen, China lizhongbao@jmu.edu.cn.
Z B
ZB
Li
Fisheries College, Jimei University, Xiamen, China.|Fujian Provincial Key Laboratory of Marine Fishery Resources and Eco-Environment, Xiamen, China.
J B
JB
Shangguan
Fisheries College, Jimei University, Xiamen, China.|Fujian Provincial Key Laboratory of Marine Fishery Resources and Eco-Environment, Xiamen, China.
Y F
YF
Ning
Fisheries College, Jimei University, Xiamen, China.|Fujian Provincial Key Laboratory of Marine Fishery Resources and Eco-Environment, Xiamen, China.
Y
Y
Yuan
Fisheries College, Jimei University, Xiamen, China.|Fujian Provincial Key Laboratory of Marine Fishery Resources and Eco-Environment, Xiamen, China.
Y S
YS
Huang
Fisheries College, Jimei University, Xiamen, China.|Fujian Provincial Key Laboratory of Marine Fishery Resources and Eco-Environment, Xiamen, China.
X Q
XQ
Mao
D000818:Animals; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D056426:Genetic Loci; D005820:Genetic Testing; D018895:Microsatellite Repeats / Q000235:genetics*; D010473:Perciformes / Q000235:genetics*
D004247:DNA