Comparative analysis of the interaction of HSPs in dendritic cells, macrophages, RGM-1 cells infected byHelicobacter pylori.
Helicobacter pylorimay cause chronic gastritis, even gastric cancer, however, antigen-presenting cells (APCs) are most important immune cells involved in the induction and expression of the underlying inflammatory responses to resistH. pylori. To study the interaction of HSPs in dendritic cells (DCs), macrophages and RGM-1 cells infected withH. pylori, HSP-27, HSP-60, HSP-70 and HSP-90 proteins were analyzed in the mucosa tissue or serum of gastritis patients caused byH. pylori, and in cell supernatant of DCs, macrophages, RGM-1 infected byH. pylori, or in above host cells. We found that HSP-27, HSP-60, HSP-70 and HSP-90 decreased in gastric epithelial cells, but increased significantly in DCs, macrophages. Meanwhile, inflammation associated proteins iNOS-2 and COX-2 were participated in the expression of HSPs in the process of host cells defensing againstH. pyloriinfection. These findings contribute to understand the functions of HSP-27, HSP-60, HSP-70 and HSP-90 inH. pyloriinfection APCs and gastric epithelial cells indicating that HSPs would be diagnostic markers forH. pyloriinfection.
Department of Clinical Laboratory, Kunshan First People's Hospital, Affiliated to Jiangsu University Kunshan 215300, People's Republic of China.
Yongliang
Y
Yao
Department of Clinical Laboratory, Kunshan First People's Hospital, Affiliated to Jiangsu University Kunshan 215300, People's Republic of China.
Jianhong
J
Wu
Department of Clinical Laboratory, Kunshan First People's Hospital, Affiliated to Jiangsu University Kunshan 215300, People's Republic of China.
Tao
T
Gu
Department of Clinical Laboratory, Kunshan First People's Hospital, Affiliated to Jiangsu University Kunshan 215300, People's Republic of China.
Yang
Y
Cheng
Department of Pathology, Chongqing Cancer Institute Chongqing 400030, People's Republic of China.
Guangxin
G
Li
HSPs; Helicobacter pylori; RGM-1; dendritic cells; macrophages