Frankia inefficax sp. nov., an actinobacterial endophyte inducing ineffective, non nitrogen-fixing, root nodules on its actinorhizal host plants.
Strain EuI1cTis the first actinobacterial endophyte isolated from Elaeagnus umbellata that was shown to be infective on members of Elaeagnaceae and Morella but lacking the ability to form effective root nodules on its hosts. The strain can be easily distinguished from strains of other Frankia species based on its inability to produce vesicles, the specialized thick-walled structures where nitrogen fixation occurs. Chemotaxonomically, strain EuI1cTcontains phosphatidylinositol, diphosphatidylglycerol, two glycophospholipids and phosphatidylglycerol as phospholipids. The whole cell sugars were composed of glucose, galactose, mannose, ribose, rhamnose and fucose as diagnostic sugars of the species. Major fatty acids were iso-C16:0, C17:1ω8c and C15:0and C17:0and the predominant menaquinones were MK-9(H6), MK-9(H8) and MK-9(H4). Analysis of the 16S rRNA gene sequence of strain EuI1cTshowed 97, 97.4 and 97.9% identity with Frankia elaeagni DSM 46783T, Frankia casuarinae DSM 45818Tand Frankia alni DSM 45986T, respectively. Digital DNA:DNA hybridizations with type strains of the three Frankia species with validly/effectively published names are significantly below 70%. These results warrant distinction of EuI1cT(= DSM 45817T= CECT 9037T) as the type strain of a novel species designated Frankia inefficax sp. nov.
Laboratoire Microorganismes et Biomolécules Actives, Université Tunis El Manar (FST) & Université de Carthage (INSAT), 2092, Tunis, Tunisia.|School of Biology, Newcastle University, Ridley Building 2, Newcastle upon Tyne, NE1 7RU, UK.
Imen
I
Nouioui
Laboratoire Microorganismes et Biomolécules Actives, Université Tunis El Manar (FST) & Université de Carthage (INSAT), 2092, Tunis, Tunisia.
Faten
F
Ghodhbane-Gtari
School of Biology, Newcastle University, Ridley Building 2, Newcastle upon Tyne, NE1 7RU, UK.
Maria
M
Del Carmen Montero-Calasanz
Central Facility for Microscopy, HZI - Helmholtz Centre for Infection Research, Inhoffenstraße 7, 38124, Braunschweig, Germany.
Manfred
M
Rohde
Department of Molecular, Cellular, and Biomedical Sciences, University of New Hampshire, 289 Rudman Hall, 46 college Road, Durham, NH, 03824-2617, USA.
Louis S
LS
Tisa
Laboratoire Microorganismes et Biomolécules Actives, Université Tunis El Manar (FST) & Université de Carthage (INSAT), 2092, Tunis, Tunisia. maher.gtari@fst.rnu.tn.
Maher
http://orcid.org/0000-0001-6946-6907
M
Gtari
School of Biology, Newcastle University, Ridley Building 2, Newcastle upon Tyne, NE1 7RU, UK.
Hans-Peter
HP
Klenk
D015373:Bacterial Typing Techniques; D002241:Carbohydrates / Q000032:analysis; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D060026:Endophytes / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D005227:Fatty Acids / Q000032:analysis; D040161:Frankia / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D009586:Nitrogen Fixation; D010743:Phospholipids / Q000032:analysis; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D053204:Root Nodules, Plant / Q000382:microbiology*; D012988:Soil Microbiology; D013045:Species Specificity; D013559:Symbiosis; D064028:Tracheophyta / Q000382:microbiology
D002241:Carbohydrates; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S
Chemotaxonomy; Frankia; Ineffective symbiosis; Phylogeny; Root nodules