Novel efficient promoter of the mitochondrial porin, voltage-dependent anion channel (VDAC), in the genome of the Yarrowia lipolytica yeast.
This article presents the characteristics of the highly inducible promoter of the gene encoding the mitochondrial porin, the voltage-dependent anion channel (VDAC). This promoter is recommended for use in new genetic constructs both in basic research for assessing the adaptive strategy of lower eukaryotes under adverse conditions and in designing new highly competitive transformants producing economically important compounds (proteins, lipids, and organic acids) on its basis.
Mendeleev University of Chemical Technology, Miusskaya pl. 9, Moscow, 125190, Russia.
F F
FF
Kulanbaewa
Bach Institute of Biochemistry, Federal Research Center "Fundamentals of Biotechnology,", Russian Academy of Sciences, Leninskii pr. 33, Moscow, 119071, Russia.
V Yu
VY
Sekova
Bach Institute of Biochemistry, Federal Research Center "Fundamentals of Biotechnology,", Russian Academy of Sciences, Leninskii pr. 33, Moscow, 119071, Russia.
E P
EP
Isakova
Bach Institute of Biochemistry, Federal Research Center "Fundamentals of Biotechnology,", Russian Academy of Sciences, Leninskii pr. 33, Moscow, 119071, Russia. yul_der@mail.ru.
Y I
YI
Deryabina
Schmidt Joint Institute of Physics of the Earth, Russian Academy of Sciences, ul. Bol'shaya Gruzinskaya 10, Moscow, 123810, Russia.
A V
AV
Nikolaev
D003470:Culture Media / Q000737:chemistry; D018014:Gene Transfer Techniques; D017930:Genes, Reporter; D005818:Genetic Engineering / Q000379:methods; D005822:Genetic Vectors; D016678:Genome; D006861:Hydrogen Peroxide / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D007763:Lac Operon; D011401:Promoter Regions, Genetic; D013312:Stress, Physiological; D050994:Voltage-Dependent Anion Channels / Q000235:genetics* / Q000378:metabolism; D025062:Yarrowia / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D001616:beta-Galactosidase / Q000235:genetics / Q000378:metabolism
D003470:Culture Media; D050994:Voltage-Dependent Anion Channels; D006861:Hydrogen Peroxide; D001616:beta-Galactosidase