Reactive electrophilic oxylipins trigger a heat stress-like response through HSFA1 transcription factors.
Abiotic and biotic stresses are often characterized by an induction of reactive electrophile species (RES) such as the jasmonate 12-oxo-phytodienoic acid (OPDA) or the structurally related phytoprostanes. Previously, RES oxylipins have been shown massively to induce heat-shock-response (HSR) genes including HSP101 chaperones. Moreover, jasmonates have been reported to play a role in basal thermotolerance. We show that representative HSR marker genes are strongly induced by RES oxylipins through the four master regulator transcription factors HSFA1a, b, d, and e essential for short-term adaptation to heat stress in Arabidopsis. When compared with Arabidopsis seedlings treated at the optimal acclimation temperature of 37 °C, the exogenous application of RES oxylipins at 20 °C induced a much weaker induction of HSP101 at both the gene and protein expression levels which, however, was not sufficient to confer short-term acquired thermotolerance. Moreover, jasmonate-deficient mutant lines displayed a wild-type-like HSR and were not compromised in acquiring thermotolerance. Hence, the OPDA- and RES oxylipin-induced HSR is not sufficient to protect seedlings from severe heat stress but may help plants to cope better with stresses associated with protein unfolding by inducing a battery of chaperones in the absence of heat.
Julius-von-Sachs-Institute of Biosciences, Biocenter, Pharmaceutical Biology, University of Wuerzburg, D-97082 Wuerzburg, Germany.
Miriam
M
Muench
Julius-von-Sachs-Institute of Biosciences, Biocenter, Pharmaceutical Biology, University of Wuerzburg, D-97082 Wuerzburg, Germany.|* Present address: Department of Pharmacology-Clinical Pharmacology Unit, University Hospital of Cologne, D-50931 Cologne, Germany.
Chih-Hsuan
CH
Hsin
Julius-von-Sachs-Institute of Biosciences, Biocenter, Pharmaceutical Biology, University of Wuerzburg, D-97082 Wuerzburg, Germany.
Elena
E
Ferber
Julius-von-Sachs-Institute of Biosciences, Biocenter, Pharmaceutical Biology, University of Wuerzburg, D-97082 Wuerzburg, Germany.
Susanne
S
Berger
Julius-von-Sachs-Institute of Biosciences, Biocenter, Pharmaceutical Biology, University of Wuerzburg, D-97082 Wuerzburg, Germany martin.mueller@biozentrum.uni-wuerzburg.de.
Martin J
MJ
Mueller
D029681:Arabidopsis Proteins / Q000502:physiology; D003517:Cyclopentanes / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000502:physiology*; D000076249:Heat Shock Transcription Factors; D006360:Heat-Shock Proteins / Q000502:physiology*; D018869:Heat-Shock Response / Q000502:physiology*; D006358:Hot Temperature; D054883:Oxylipins / Q000378:metabolism*; D010937:Plant Growth Regulators / Q000502:physiology; D010940:Plant Proteins / Q000502:physiology*; D036226:Seedlings / Q000502:physiology; D014157:Transcription Factors / Q000502:physiology*; D059467:Transcriptome
D029681:Arabidopsis Proteins; D003517:Cyclopentanes; D004268:DNA-Binding Proteins; D000076249:Heat Shock Transcription Factors; D006360:Heat-Shock Proteins; D054883:Oxylipins; D010937:Plant Growth Regulators; D010940:Plant Proteins; D014157:Transcription Factors; C011006:jasmonic acid
12-oxo-phytodienoic acid; acquired thermotolerance; heat stress; jasmonates; phytoprostanes; reactive electrophilic species; unfolded protein response.