Primary cilia are sensors of electrical field stimulation to induce osteogenesis of human adipose-derived stem cells.
In this study, we report for the first time that the primary cilium acts as a crucial sensor for electrical field stimulation (EFS)-enhanced osteogenic response in osteoprogenitor cells. In addition, primary cilia seem to functionally modulate effects of EFS-induced cellular calcium oscillations. Primary cilia are organelles that have recently been implicated to play a crucial sensor role for many mechanical and chemical stimuli on stem cells. Here, we investigate the role of primary cilia in EFS-enhanced osteogenic response of human adipose-derived stem cells (hASCs) by knocking down 2 primary cilia structural proteins, polycystin-1 and intraflagellar protein-88. Our results indicate that structurally integrated primary cilia are required for detection of electrical field signals in hASCs. Furthermore, by measuring changes of cytoplasmic calcium concentration in hASCs during EFS, our findings also suggest that primary cilia may potentially function as a crucial calcium-signaling nexus in hASCs during EFS.-Cai, S., Bodle, J. C., Mathieu, P. S., Amos, A., Hamouda, M., Bernacki, S., McCarty, G., Loboa, E. G. Primary cilia are sensors of electrical field stimulation to induce osteogenesis of human adipose-derived stem cells.
Department of Materials Science and Engineering, North Carolina State University, Raleigh, North Carolina, USA.
Shaobo
S
Cai
Joint Department of Biomedical Engineering, North Carolina State University and University of North Carolina at Chapel Hill, Raleigh, North Carolina, USA; and.
Josephine C
JC
Bodle
Joint Department of Biomedical Engineering, North Carolina State University and University of North Carolina at Chapel Hill, Raleigh, North Carolina, USA; and.
Pattie S
PS
Mathieu
Joint Department of Biomedical Engineering, North Carolina State University and University of North Carolina at Chapel Hill, Raleigh, North Carolina, USA; and.
Alison
A
Amos
Joint Department of Biomedical Engineering, North Carolina State University and University of North Carolina at Chapel Hill, Raleigh, North Carolina, USA; and.
Mehdi
M
Hamouda
Joint Department of Biomedical Engineering, North Carolina State University and University of North Carolina at Chapel Hill, Raleigh, North Carolina, USA; and.
Susan
S
Bernacki
Joint Department of Biomedical Engineering, North Carolina State University and University of North Carolina at Chapel Hill, Raleigh, North Carolina, USA; and.
Greg
G
McCarty
Joint Department of Biomedical Engineering, North Carolina State University and University of North Carolina at Chapel Hill, Raleigh, North Carolina, USA; and egloboa@missouri.edu.|College of Engineering, University of Missouri, Columbia, Missouri, USA.
Elizabeth G
EG
Loboa
D000273:Adipose Tissue / Q000166:cytology*; D015415:Biomarkers; D002118:Calcium / Q000378:metabolism; D002470:Cell Survival; D002478:Cells, Cultured; D002923:Cilia / Q000502:physiology*; D004558:Electric Stimulation; D005786:Gene Expression Regulation / Q000502:physiology; D006801:Humans; D010012:Osteogenesis / Q000502:physiology*; D034622:RNA Interference; D034741:RNA, Small Interfering; D013234:Stem Cells / Q000502:physiology*
D015415:Biomarkers; D034741:RNA, Small Interfering; D002118:Calcium
calcium oscillations; intraflagellar protein-88; polycystin-1; siRNA knockdown; signal transduction