Upregulation of long non-coding RNA LINC00152 by SP1 contributes to gallbladder cancer cell growth and tumor metastasis via PI3K/AKT pathway.
Gallbladder cancer (GBC) is one of the most lethal cancers with poor prognosis. In this study, we report that the long non-coding RNA LINC00152 is significantly upregulated in GBC tissues and cell lines. The high LINC00152 levels correlated positively with tumor status progression, lymph node invasion and TNM stage advancement. Functionally, we revealed that LINC00152 dramatically promoted cell proliferation, metastasis and inhibited apoptosisin vitro.In vivo, LINC00152 overexpression significantly promoted tumor growth. Mechanistic analyses indicated that LINC00152 could participate in the phosphatidylinositol 3-kinase (PI3K)/AKT signaling pathway, and transcription factor specificity protein 1 (SP1) induces its overexpression. In summary, our findings suggest that LINC00152 contributes to the oncogenic potential of GBC and SP1/LINC00152/PI3K/AKT may be a potential therapeutic target for GBC.
Department of General Surgery, Xinhua Hospital, School of Medicine, Shanghai Jiaotong University Shanghai 200092, China.
Qiang
Q
Cai
Shanghai Key Laboratory of Gastric Neoplasms, Department of Surgery, Shanghai Institute of Digestive Surgery, Ruijin Hospital, School of Medicine, Shanghai Jiaotong University Shanghai 200025, China.
Zhen-Qiang
ZQ
Wang
Department of General Surgery, Xinhua Hospital, School of Medicine, Shanghai Jiaotong University Shanghai 200092, China.
Shou-Hua
SH
Wang
Shanghai Key Laboratory of Gastric Neoplasms, Department of Surgery, Shanghai Institute of Digestive Surgery, Ruijin Hospital, School of Medicine, Shanghai Jiaotong University Shanghai 200025, China.
Chen
C
Li
Shanghai Key Laboratory of Gastric Neoplasms, Department of Surgery, Shanghai Institute of Digestive Surgery, Ruijin Hospital, School of Medicine, Shanghai Jiaotong University Shanghai 200025, China.
Zheng-Gang
ZG
Zhu
Department of General Surgery, Xinhua Hospital, School of Medicine, Shanghai Jiaotong University Shanghai 200092, China.
Zhi-Wei
ZW
Quan
Department of General Surgery, Xinhua Hospital, School of Medicine, Shanghai Jiaotong University Shanghai 200092, China.
Wen-Jie
WJ
Zhang
LINC00152; Long non-coding RNA; PI3K/AKT; SP1; gallbladder cancer; transcription factor