HPLC-based metabolomics to identify cytotoxic compounds from Plectranthus amboinicus (Lour.) Spreng against human breast cancer MCF-7Cells.
The objective of this study was to identify the active compounds in Plectranthus amboinicus (Lour.) Spreng which play a role to inhibit viability of breast cancer MCF-7 cells using HPLC-based metabolomics approach. Five fractions of the plant extract were observed including ethanol, hexane, chloroform, ethyl acetate and water fraction. There were 45 HPLC chromatograms resulted from 5 fractions with 3 replications and 3 wavelengths detection. The chromatograms were compared to the data of IC50from MTT assay of each fraction against human breast cancer MCF-7 cells using metabolomics. The OPLS analysis result promptly pointed towards a chloroform fraction at retention time of 40.16-41.28min that has the greatest contribution to the cytotoxic activity. The data of mass spectra indicated that an abietane diterpene namely 7-acetoxy-6-hydroxyroyleanone was the main compound that contributed to the cytotoxic activity. This metabolomics application method can be used as a quick preliminary guideline to uncover the most dominant compound related to the bioactivity.
Department of Food Science and Technology, Bogor Agricultural University, Bogor, Indonesia.
Wahid
W
Yulianto
Department of Food Science and Technology, Bogor Agricultural University, Bogor, Indonesia; Southeast Asian Food and Agricultural Science and Technology (SEAFAST) Center, Bogor Agricultural University, Bogor, Indonesia. Electronic address: nuri@seafast.org.
Nuri
N
Andarwulan
Department of Food Science and Technology, Bogor Agricultural University, Bogor, Indonesia; Southeast Asian Food and Agricultural Science and Technology (SEAFAST) Center, Bogor Agricultural University, Bogor, Indonesia.
Puspo Edi
PE
Giriwono
Department of Animal Disease Science and Veterinary Medicine, Bogor Agricultural University, Bogor, Indonesia; Primate Research Center, Bogor Agricultural University, Bogor, Indonesia.
Joko
J
Pamungkas
D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000627:therapeutic use / Q000633:toxicity*; D001943:Breast Neoplasms / Q000188:drug therapy*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005260:Female; D006801:Humans; D061986:MCF-7 Cells; D055432:Metabolomics / Q000379:methods*; D010936:Plant Extracts / Q000737:chemistry / Q000627:therapeutic use / Q000633:toxicity*; D018515:Plant Leaves / Q000737:chemistry / Q000378:metabolism; D031346:Plectranthus / Q000737:chemistry* / Q000378:metabolism; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods
D000972:Antineoplastic Agents, Phytogenic; D010936:Plant Extracts
Bioactive compounds; Cytotoxicity; Metabolomics; Plectranthus amboinicus (Lour.) Spreng