A Proximity-Based Programmable DNA Nanoscale Assembly Line.
The assembly line is one of the key features of industrial production on the macroscopic scale, allowing programmability and sequential addition of parts to a final product. In this chapter, we use DNA to extend this notion to the nanoscale by the judicious combination of three DNA-based components: a DNA origami tile that provides a framework and track for the assembly process, three two-state DNA cassettes that can be programmed to donate cargo and are attached to the tile, and a DNA walker that can move on the track to collect cargo.
Fudan University Shanghai Cancer Center, Institutes of Biomedical Sciences, Shanghai Medical College of Fudan University, Shanghai, 200032, China.|Kidney and Dialysis Institute of Shanghai, Shanghai, 200032, China.|Kidney and Blood Purification Laboratory of Shanghai, Shanghai, 200032, China.|Department of Nephrology, Zhongshan Hospital, Shanghai Medical College, Fudan University, Shanghai, 200032, China.
Xiaoyan
X
Zhang
Kidney and Dialysis Institute of Shanghai, Shanghai, 200032, China.|Kidney and Blood Purification Laboratory of Shanghai, Shanghai, 200032, China.|Department of Nephrology, Zhongshan Hospital, Shanghai Medical College, Fudan University, Shanghai, 200032, China.
Xiaoqiang
X
Ding
Kidney and Dialysis Institute of Shanghai, Shanghai, 200032, China.|Kidney and Blood Purification Laboratory of Shanghai, Shanghai, 200032, China.|Department of Nephrology, Zhongshan Hospital, Shanghai Medical College, Fudan University, Shanghai, 200032, China.
Jianzhou
J
Zou
Kidney and Dialysis Institute of Shanghai, Shanghai, 200032, China. hongzhou.gu@fudan.edu.cn.|Kidney and Blood Purification Laboratory of Shanghai, Shanghai, 200032, China. hongzhou.gu@fudan.edu.cn.|Department of Nephrology, Zhongshan Hospital, Shanghai Medical College, Fudan University, Shanghai, 200032, China. hongzhou.gu@fudan.edu.cn.
Hongzhou
H
Gu
D004247:DNA / Q000737:chemistry*; D018625:Microscopy, Atomic Force / Q000379:methods; D049329:Nanostructures / Q000737:chemistry; D036103:Nanotechnology / Q000379:methods; D009690:Nucleic Acid Conformation
D004247:DNA
Assembly line; DNA cassettes; DNA origami; DNA walker