Number of blastocysts biopsied as a predictive indicator to obtain at least one normal/balanced embryo following preimplantation genetic diagnosis with single nucleotide polymorphism microarray in translocation cases.
OBJECTIVE
The aim of this study is to investigate the minimum number of blastocysts for biopsy to increase the likelihood of obtaining at least one normal/balanced embryo in preimplantation genetic diagnosis (PGD) for translocation carriers.


METHODS
This blinded retrospective study included 55 PGD cycles for Robertsonian translocation (RT) and 181 cycles for reciprocal translocation (rcp) to indicate when only one of the couples carried a translocation. Single-nucleotide polymorphism microarray after trophectoderm biopsy was performed.


RESULTS
Reliable results were obtained for 355/379 (93.7 %) biopsied blastocysts in RT group and 986/1053 (93.6 %) in rcp group. Mean numbers of biopsied embryos per patient, normal/balanced embryos per patient, and mean normal/balanced embryo rate per patient were 7.4, 3.1, and 40.7 % in RT group and 8.0, 2.1, and 27.3 %, respectively, in rcp group. In a regression model, three factors significantly affected the number of genetically transferrable embryos: number of biopsied embryos (P = 0.001), basal FSH level (P = 0.040), and maternal age (P = 0.027). ROC analysis with a cutoff of 1.5 was calculated for the number of biopsied embryos required to obtain at least one normal/balanced embryo for RT carriers. For rcp carriers, the cutoff was 3.5. The clinical pregnancy rate per embryo transfer was 44.2 and 42.6 % in RT and rcp groups (P = 0.836).


CONCLUSIONS
The minimum numbers of blastocysts to obtain at least one normal/balanced embryo for RT and rcp were 2 and 4 under the conditions of female age < 37 years with a basal FSH level < 11.4 IU/L.
Reproductive Medical Center, The First Affiliated Hospital of Sun Yat-sen University, (Floor 1, Building 6th) ZhongshanEr Road No. 58, Yuexiu District, Guangzhou, Guangdong, 510080, China.|Guangdong Provincial Key Laboratory of Reproductive Medicine, Guangzhou, China.
Yi-Zi
YZ
Wang
Reproductive Medical Center, The First Affiliated Hospital of Sun Yat-sen University, (Floor 1, Building 6th) ZhongshanEr Road No. 58, Yuexiu District, Guangzhou, Guangdong, 510080, China.|Guangdong Provincial Key Laboratory of Reproductive Medicine, Guangzhou, China.
Chen-Hui
CH
Ding
Reproductive Medical Center, The First Affiliated Hospital of Sun Yat-sen University, (Floor 1, Building 6th) ZhongshanEr Road No. 58, Yuexiu District, Guangzhou, Guangdong, 510080, China.|Guangdong Provincial Key Laboratory of Reproductive Medicine, Guangzhou, China.
Jing
J
Wang
Reproductive Medical Center, The First Affiliated Hospital of Sun Yat-sen University, (Floor 1, Building 6th) ZhongshanEr Road No. 58, Yuexiu District, Guangzhou, Guangdong, 510080, China.|Guangdong Provincial Key Laboratory of Reproductive Medicine, Guangzhou, China.
Yan-Hong
YH
Zeng
Reproductive Medical Center, The First Affiliated Hospital of Sun Yat-sen University, (Floor 1, Building 6th) ZhongshanEr Road No. 58, Yuexiu District, Guangzhou, Guangdong, 510080, China.|Guangdong Provincial Key Laboratory of Reproductive Medicine, Guangzhou, China.
Wen
W
Zhou
Reproductive Medical Center, The First Affiliated Hospital of Sun Yat-sen University, (Floor 1, Building 6th) ZhongshanEr Road No. 58, Yuexiu District, Guangzhou, Guangdong, 510080, China.|Guangdong Provincial Key Laboratory of Reproductive Medicine, Guangzhou, China.
Rong
R
Li
Reproductive Medical Center, The First Affiliated Hospital of Sun Yat-sen University, (Floor 1, Building 6th) ZhongshanEr Road No. 58, Yuexiu District, Guangzhou, Guangdong, 510080, China. zhoucanquan@hotmail.com.|Guangdong Provincial Key Laboratory of Reproductive Medicine, Guangzhou, China. zhoucanquan@hotmail.com.
Can-Quan
CQ
Zhou
Reproductive Medical Center, The First Affiliated Hospital of Sun Yat-sen University, (Floor 1, Building 6th) ZhongshanEr Road No. 58, Yuexiu District, Guangzhou, Guangdong, 510080, China. dengmfen@163.com.|Guangdong Provincial Key Laboratory of Reproductive Medicine, Guangzhou, China. dengmfen@163.com.
Ming-Fen
MF
Deng
Reproductive Medical Center, The First Affiliated Hospital of Sun Yat-sen University, (Floor 1, Building 6th) ZhongshanEr Road No. 58, Yuexiu District, Guangzhou, Guangdong, 510080, China. xuyanwen@live.cn.|Guangdong Provincial Key Laboratory of Reproductive Medicine, Guangzhou, China. xuyanwen@live.cn.
Yan-Wen
YW
Xu
D000328:Adult; D001706:Biopsy; D001755:Blastocyst / Q000166:cytology*; D004624:Embryo Transfer; D005260:Female; D005307:Fertilization in Vitro; D005640:Follicle Stimulating Hormone / Q000378:metabolism; D006801:Humans; D008423:Maternal Age; D020641:Polymorphism, Single Nucleotide; D011247:Pregnancy; D018873:Pregnancy Rate; D019836:Preimplantation Diagnosis; D014178:Translocation, Genetic
D005640:Follicle Stimulating Hormone
Preimplantation genetic diagnosis; Reciprocal translocation; Robertsonian translocation; Single-nucleotide polymorphism; Trophectoderm biopsy