RANTES Gene Polymorphisms Associated with HIV-1 Infections in Kenyan Population.
Previous studies have reported that two single nucleotide polymorphisms (SNPs) in the RANTES gene promoter region, -403G/A and -28C/G, are associated with a slower rate of decline in CD4+ T cell count. In addition, as a ligand of the major HIV coreceptor CCR5, it is known to block HIV-CCR5 interactions in the course of the HIV infection cycle. This study was carried out with the aim of determining the occurrence of single nucleotide polymorphisms (SNPs) -403G > A and -28C > G in the promoter region of RANTES, in a subset of the Kenyan population. Genomic DNA was extracted from peripheral blood monocular cells and used to amplify the RANTES gene region. Restriction fragment length polymorphism was used to determine the genotypes of the RANTES gene. Out of 100 HIV infected individuals, 19% had G1 genotypes (403G/G, 28C/G), 30% (403A/A, 28C/C), and 50% (403G/A, 28C/C), while in healthy blood donors 13% had G4 (403G/A, 28C/C) genotypes, 22% (403A/A, 28C/C), and 54% (403G/A, 28C/C). HIV negative blood donors (54%) had higher risk of alteration to risk of HIV transmission compared to those who were HIV infected (50%). However, the risk to transmission and distribution differences was not significant (P= 0.092). The study showed that RANTES polymorphisms -403 and -28 alleles do exist in the Kenyan population.
Institute of Tropical Medicine and Infectious Diseases, Jomo Kenyatta University of Agriculture and Technology, Juja, Kenya.
Shem P M
SP
Mutuiri
Institute of Tropical Medicine and Infectious Diseases, Jomo Kenyatta University of Agriculture and Technology, Juja, Kenya.
Helen L
HL
Kutima
Institute of Tropical Medicine and Infectious Diseases, Jomo Kenyatta University of Agriculture and Technology, Juja, Kenya.
Lamech M
0000-0003-1048-1787
LM
Mwapagha
Institute of Biotechnology, Jomo Kenyatta University of Agriculture and Technology, Juja, Kenya.
James K
JK
Munyao
Department of Plant and Microbial Sciences, Kenyatta University, Nairobi, Kenya.
Anthony Kebira
AK
Nyamache
Department of Plant and Microbial Sciences, Kenyatta University, Nairobi, Kenya.
Irene
I
Wanjiru
Center for Virus Research, Kenya Medical Research Institute, Nairobi, Kenya.
Samoel A
0000-0003-3050-5977
SA
Khamadi
D000293:Adolescent; D000328:Adult; D015415:Biomarkers / Q000378:metabolism*; D016022:Case-Control Studies; D018946:Chemokine CCL5 / Q000235:genetics*; D005260:Female; D005500:Follow-Up Studies; D020022:Genetic Predisposition to Disease; D005838:Genotype; D015658:HIV Infections / Q000453:epidemiology* / Q000235:genetics* / Q000821:virology; D015497:HIV-1 / Q000502:physiology*; D006801:Humans; D007630:Kenya / Q000453:epidemiology; D008297:Male; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D011379:Prognosis; D055815:Young Adult
D015415:Biomarkers; C483024:CCL5 protein, human; D018946:Chemokine CCL5