Ionizing radiation affects profile of serum metabolites: increased level of 3-hydroxybutyric acid in serum of cancer patients treated with radiotherapy.
Radiotherapy causes molecular changes observed at the level of body fluids, which are potential biomarker candidates for assessment of radiation exposure. Here we analyzed radiotherapy-induced changes in a profile of small metabolites detected in sera of head and neck cancer patients using the gas chromatography coupled with mass spectrometry approach. There were about 20 compounds, including carboxylic acids, sugars, amines and amino acids, whose levels significantly differed between pre-treatment and post-treatment samples. Among metabolites upregulated by radiotherapy there was 3-hydroxybutyric acid, whose level increased about three times in post-treatment samples. Moreover, compounds affected by irradiation were associated with several metabolic pathways, including protein biosynthesis and amino acid metabolism.
Maria Skłodowska-Curie Memorial Cancer Center and Institute of Oncology, Gliwice, Poland.
Małgorzata
M
Roś-Mazurczyk
Maria Skłodowska-Curie Memorial Cancer Center and Institute of Oncology, Gliwice, Poland.
Anna
A
Wojakowska
Institute of Bioorganic Chemistry Polish Academy of Sciences, Poznań, Poland.
Łukasz
Ł
Marczak
Warwick Systems Biology Centre, University of Warwick, Warwick, United Kingdom.
Krzysztof
K
Polański
Maria Skłodowska-Curie Memorial Cancer Center and Institute of Oncology, Gliwice, Poland.
Monika
M
Pietrowska
Maria Skłodowska-Curie Memorial Cancer Center and Institute of Oncology, Gliwice, Poland.
Karol
K
Jelonek
Maria Skłodowska-Curie Memorial Cancer Center and Institute of Oncology, Gliwice, Poland.
Iwona
I
Domińczyk
Maria Skłodowska-Curie Memorial Cancer Center and Institute of Oncology, Gliwice, Poland.
Agata
A
Hajduk
Maria Skłodowska-Curie Memorial Cancer Center and Institute of Oncology, Gliwice, Poland.
Tomasz
T
Rutkowski
Maria Skłodowska-Curie Memorial Cancer Center and Institute of Oncology, Gliwice, Poland.
Krzysztof
K
Składowski
Maria Skłodowska-Curie Memorial Cancer Center and Institute of Oncology, Gliwice, Poland.
Piotr
P
Widłak
D020155:3-Hydroxybutyric Acid / Q000097:blood / Q000378:metabolism* / Q000528:radiation effects; D000328:Adult; D000368:Aged; D015415:Biomarkers; D002294:Carcinoma, Squamous Cell / Q000378:metabolism / Q000532:radiotherapy*; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry; D006258:Head and Neck Neoplasms / Q000378:metabolism / Q000532:radiotherapy*; D006801:Humans; D008297:Male; D053858:Metabolic Networks and Pathways / Q000528:radiation effects; D055432:Metabolomics / Q000379:methods; D008875:Middle Aged; D000069079:Radiation Exposure / Q000032:analysis; D011839:Radiation, Ionizing; D044967:Serum / Q000378:metabolism* / Q000528:radiation effects; D015854:Up-Regulation / Q000528:radiation effects
D015415:Biomarkers; D020155:3-Hydroxybutyric Acid
head and neck cancer; mass spectrometry; metabolomics; radiation response; serum metabolome