Tartary buckwheat FtMYB10 encodes an R2R3-MYB transcription factor that acts as a novel negative regulator of salt and drought response in transgenic Arabidopsis.
Tartary buckwheat is a strongly abiotic, resistant coarse cereal, but its tolerance mechanisms for stress are largely unknown. MYB transcription factors play key roles in various physiological, biochemical and molecular responses, which can both positively and negatively regulate the stress tolerance of plants. In this study, we report that the expression of FtMYB10, a R2R3-MYB gene from Tartary buckwheat, was induced significantly by ABA and drought treatments. A seed germination test under ABA treatment indicated that transgenic lines were less sensitive to ABA. The overexpression of FtMYB10 in Arabidopsis reduced drought and salt tolerance. Further studies showed that the proline contents in the transgenic plants are markedly decreased associated with reduced expression of the P5CS1 gene under both normal and stress conditions. Furthermore, the expression of some stress-responsive genes, including DREB1/CBFs, RD29B, RD22, and several genes of the DRE/CRT class, decreased in response to FtMYB10 overexpression in Arabidopsis. These results suggest that FtMYB10 may play a key role in ABA signaling feedback regulation and act as a novel negative regulator of salt and drought stress tolerance in plants.
College of Life Science, Sichuan Agricultural University, No. 46, Xinkang Road, Ya'an, 625014, Sichuan Province, China.
Fei
F
Gao
College of Life Science, Sichuan Agricultural University, No. 46, Xinkang Road, Ya'an, 625014, Sichuan Province, China.
Huipeng
H
Yao
College of Life Science, Sichuan Agricultural University, No. 46, Xinkang Road, Ya'an, 625014, Sichuan Province, China.
Haixia
H
Zhao
College of Life Science, Sichuan Agricultural University, No. 46, Xinkang Road, Ya'an, 625014, Sichuan Province, China.
Jing
J
Zhou
College of Life Science, Sichuan Agricultural University, No. 46, Xinkang Road, Ya'an, 625014, Sichuan Province, China.
Xiaopeng
X
Luo
College of Life Science, Sichuan Agricultural University, No. 46, Xinkang Road, Ya'an, 625014, Sichuan Province, China.
Yunji
Y
Huang
College of Life Science, Sichuan Agricultural University, No. 46, Xinkang Road, Ya'an, 625014, Sichuan Province, China.
Chenglei
C
Li
College of Life Science, Sichuan Agricultural University, No. 46, Xinkang Road, Ya'an, 625014, Sichuan Province, China.
Hui
H
Chen
College of Life Science, Sichuan Agricultural University, No. 46, Xinkang Road, Ya'an, 625014, Sichuan Province, China. Electronic address: wuqi@sicau.edu.cn.
Qi
Q
Wu
D000040:Abscisic Acid / Q000494:pharmacology; D000064:Acclimatization / Q000235:genetics; D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism; D055864:Droughts; D019612:Fagopyrum / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism*; D015870:Gene Expression; D017343:Genes, Plant; D010802:Phylogeny; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism*; D030821:Plants, Genetically Modified; D055049:Salt Tolerance / Q000235:genetics; D017386:Sequence Homology, Amino Acid; D013312:Stress, Physiological; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism*
D010940:Plant Proteins; D014157:Transcription Factors; D000040:Abscisic Acid
ABA; Abiotic stress; Negative regulator; R2R3-MYB; Tartary buckwheat; Transgenic Arabidopsis