Isolation of Hepatocytes and Stellate Cells from a Single Piece of Human Liver.
Co-transplantation of hepatocytes and hepatic stellate cells has been shown to increase the engraftment of transplanted hepatocytes in the liver. Here, we describe a method for the simultaneous isolation of human primary hepatocytes and hepatic stellate cells from the same donor for co-transplantation or for use in in vitro cell culture models.
Department of General, Visceral and Transplantation Surgery, Hospital of the LMU Munich, Munich, Germany.
Serene M L
SM
Lee
Department of General, Visceral and Transplantation Surgery, Hospital of the LMU Munich, Munich, Germany.|Biobank under the administration of the Human Tissue and Cell Research Foundation, Department of General, Visceral and Transplantation Surgery, Hospital of the LMU Munich, Munich, Germany.
Tobias S
TS
Schiergens
Department of General, Visceral and Transplantation Surgery, Hospital of the LMU Munich, Munich, Germany.|Hepacult GmbH, Munich, Germany.
Maresa
M
Demmel
Biobank under the administration of the Human Tissue and Cell Research Foundation, Department of General, Visceral and Transplantation Surgery, Hospital of the LMU Munich, Munich, Germany.
Reinhard M K
RM
Thasler
Department of General, Visceral and Transplantation Surgery, Hospital of the LMU Munich, Munich, Germany. Wolfgang.Thasler@med.uni-muenchen.de.|Biobank under the administration of the Human Tissue and Cell Research Foundation, Department of General, Visceral and Transplantation Surgery, Hospital of the LMU Munich, Munich, Germany. Wolfgang.Thasler@med.uni-muenchen.de.
Wolfgang E
WE
Thasler
D002469:Cell Separation / Q000295:instrumentation / Q000379:methods*; D002470:Cell Survival; D017690:Cell Transplantation / Q000379:methods; D002478:Cells, Cultured; D018920:Coculture Techniques / Q000379:methods; D015925:Cryopreservation; D055166:Hepatic Stellate Cells / Q000502:physiology* / Q000637:transplantation; D022781:Hepatocytes / Q000502:physiology* / Q000637:transplantation; D006801:Humans; D008099:Liver / Q000166:cytology / Q000601:surgery*; D010477:Perfusion / Q000295:instrumentation / Q000379:methods*; D061251:Primary Cell Culture / Q000379:methods; D014019:Tissue Donors
Collagenase; Hepatic stellate cells; Hepatocyte isolation; Hepatocytes; Human; Human hepatocytes; Liver; Liver cell isolation; Perfusion; Stellate cell isolation