Induction of Both Local Immune Response in Mice and Protection in a Rabbit Model by Intranasal Immunization with Modified Vaccinia Ankara Virus Expressing a Secreted Form of Bovine Herpesvirus 1 Glycoprotein D.
In this study, we evaluated the immunogenicity and efficacy of mucosal delivery of a recombinant modified vaccinia Ankara virus (MVA) expressing the secreted version of bovine herpesvirus type 1 (BoHV-1) glycoprotein D (MVA-gDs) without addition of adjuvant in two animal models. First, we demonstrated the capability of MVA-gDs of inducing both local and systemic anti-gD humoral immune response after intranasal immunization of mice. Then, we confirmed that two doses of MVA-gDs administered intranasally to rabbits induced systemic anti-gD antibodies and conferred protection against BoHV-1 challenge. Our results show the potential of using MVA as a vector for the rational design of veterinary vaccines capable of inducing specific and protective immune responses both at local and systemic level.
1 Instituto de Biotecnología, Instituto Nacional de Tecnología Agropecuaria (CICVyA-INTA) , Hurlingham, Argentina .|2 Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET) , Ciudad Autónoma de Buenos Aires, Argentina .
María Paula
MP
Del Medico Zajac
1 Instituto de Biotecnología, Instituto Nacional de Tecnología Agropecuaria (CICVyA-INTA) , Hurlingham, Argentina .|2 Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET) , Ciudad Autónoma de Buenos Aires, Argentina .
Flavia Adriana
FA
Zanetti
1 Instituto de Biotecnología, Instituto Nacional de Tecnología Agropecuaria (CICVyA-INTA) , Hurlingham, Argentina .
María Soledad
MS
Esusy
1 Instituto de Biotecnología, Instituto Nacional de Tecnología Agropecuaria (CICVyA-INTA) , Hurlingham, Argentina .|2 Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET) , Ciudad Autónoma de Buenos Aires, Argentina .
Carlos Rodolfo
CR
Federico
3 Instituto de Virología, Instituto Nacional de Tecnología Agropecuaria (CICVyA-INTA) , Hurlingham, Argentina .
Osvaldo
O
Zabal
4 Cátedra de Virología, Facultad de Ciencias Veterinarias, Universidad Nacional de La Plata , La Plata, Argentina .
Alejandro Rafael
AR
Valera
1 Instituto de Biotecnología, Instituto Nacional de Tecnología Agropecuaria (CICVyA-INTA) , Hurlingham, Argentina .
Gabriela
G
Calamante
D000281:Administration, Intranasal; D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D004195:Disease Models, Animal; D004337:Drug Carriers; D005260:Female; D006566:Herpesviridae Infections / Q000276:immunology / Q000517:prevention & control*; D007242:Herpesvirus 1, Bovine / Q000276:immunology*; D022283:Herpesvirus Vaccines / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*; D008807:Mice, Inbred BALB C; D011817:Rabbits; D014614:Vaccines, Synthetic / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology; D014616:Vaccinia virus / Q000235:genetics*; D014764:Viral Proteins / Q000235:genetics / Q000276:immunology*
D000914:Antibodies, Viral; D004337:Drug Carriers; D022283:Herpesvirus Vaccines; D014614:Vaccines, Synthetic; D014764:Viral Proteins; C046998:bovine herpesvirus type-1 glycoproteins
vaccines; vectors; veterinary