First record of Perkinsus chesapeaki infecting Crassostrea rhizophorae in South America.
This study investigated Perkinsus spp. infecting Crassostrea rhizophorae from the Jaguaribe River estuary, Ceará, Brazil. Fragments of gills and rectum of the oysters (n=150) were incubated in Ray's fluid thioglycollate medium (RFTM). Genus Perkinsus-specific PerkITS85/750 PCR assays were performed and their amplicons were sequenced by the Sanger method. The RFTM assays confirmed Perkinsus spp. The sequencing of the amplified fragments from the rDNA internal transcribed spacers (ITS) of Perkinsus spp. confirmed Perkinsus chesapeaki. Neighbor-Joining analyzes place P. chesapeaki identified in this study in a well-supported clade with other isolates of the same species. This is the first record of P. chesapeaki infecting C. rhizophorae in South America.
Instituto de Ciências do Mar, Universidade Federal do Ceará, Av. da Abolição, 3207 - Meireles, CEP: 60165-081 Fortaleza, CE, Brazil. Electronic address: maxfloyd@hotmail.com.
Maximiano Pinheiro
MP
Dantas Neto
Instituto de Ciências do Mar, Universidade Federal do Ceará, Av. da Abolição, 3207 - Meireles, CEP: 60165-081 Fortaleza, CE, Brazil. Electronic address: cvgesteira@secrel.com.br.
Tereza Cristina Vasconcelos
TC
Gesteira
Instituto Federal de Educação Ciência e Tecnologia do Ceará, Campus Aracati, Rua Teófilo Pinto, 200 - Farias Brito, CEP 62800-000 Aracati, CE, Brazil. Electronic address: rachelsabry@yahoo.com.br.
Rachel Costa
RC
Sabry
Instituto de Ciências do Mar, Universidade Federal do Ceará, Av. da Abolição, 3207 - Meireles, CEP: 60165-081 Fortaleza, CE, Brazil. Electronic address: rubensfeijo2005@yahoo.com.br.
Rubens Galdino
RG
Feijó
Instituto de Ciências do Mar, Universidade Federal do Ceará, Av. da Abolição, 3207 - Meireles, CEP: 60165-081 Fortaleza, CE, Brazil. Electronic address: jamillemforte@gmail.com.
Jamille Martins
JM
Forte
Universidade Estadual de Santa Cruz (UESC), Departamento de Ciências Biológicas (DCB), Rodovia Ilhéus-Itabuna, km 16, CEP 45652-900 Ilhéus, BA, Brazil. Electronic address: guislaboehs@hotmail.com.
Guisla
G
Boehs
Instituto de Ciências do Mar, Universidade Federal do Ceará, Av. da Abolição, 3207 - Meireles, CEP: 60165-081 Fortaleza, CE, Brazil. Electronic address: maggioni@ufc.br.
Rodrigo
R
Maggioni
D056893:Alveolata / Q000235:genetics*; D000818:Animals; D001483:Base Sequence; D001938:Brazil; D049896:Crassostrea / Q000469:parasitology*; D017125:Genes, Protozoan; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D011529:Protozoan Infections, Animal / Q000469:parasitology*
Bivalve; Crassostrea rhizophorae; Health; Perkinsus chesapeaki