title: Pharmacological importance, characterization and applications of gold and silver nanoparticles synthesized by Panax ginseng fresh leaves.
abstract: Previously, we showed the rapid and eco-friendly synthesis of gold and silver nanoparticles within 3 and 45 min by fresh leaves extract of herbal medicinal plant Panax ginseng. In addition, we characterized the nanoparticles in terms of shape, size, morphology and stability by FE-TEM, EDX, elemental mapping, SEAD, XRD and particles size analysis. In addition of this, we showed their antimicrobial, anti-coagulant, and biofilm inhibition activity of nanoparticles. Continuing our previous study, here we highlight the further characterization and biomedical applications of P. ginseng leaf-mediated gold and silver nanoparticles. We characterized the nanoparticles further in terms of active functional group and capping layer, surface charge, and temperature stability. Based on these factors, we explored the nanoparticles for antioxidant efficacy, biocompatibility in HaCaT cells, 3T3-L1 pre-adipocytes cells, for anticancer efficacy in A549 lung cancer and B16BL6 skin melenoma cancer cell lines and for anti-inflammation efficacy in RAW 264.7 cell lines. Based on our findings, we suggest that the P. ginseng-mediated gold nanoparticles have high antioxidant activity and highly biocompatibility in HaCaT cells, 3T3-L1 pre-adipocytes cells, RAW 264.7 cells lines and could be considered for future drug delivery carriers. The silver nanoparticles also showed high potent antioxidant efficacy, additionally it showed high anticancer effect in A549 lung cancer and B16BL6 skin melenoma cancer cell lines as compared to precursor salts. Moreover, both gold and silver nanoparticles have anti-inflammatory efficacies in RAW 264.7 cells. Thus, the study may provide useful insights of P. ginseng leaves extract-mediated biocompatible gold and silver nanoparticles and improving their applicability in designing nanoparticles carrier systems for drug delivery applications.
authors.affiliation: a Department of Oriental Medicine Biotechnology , Ginseng Bank College of Life Science, Kyung Hee University , Yongin-si , Gyeonggi-do , Republic of Korea.|b Graduate School of Biotechnology College of life science, Kyung Hee University , Yongin-si , Gyeonggi-do , Republic of Korea.
authors.forename: Priyanka
authors.initials: P
authors.lastname: Singh
authors.affiliation: a Department of Oriental Medicine Biotechnology , Ginseng Bank College of Life Science, Kyung Hee University , Yongin-si , Gyeonggi-do , Republic of Korea.
authors.forename: Hina
authors.initials: H
authors.lastname: Singh
authors.affiliation: a Department of Oriental Medicine Biotechnology , Ginseng Bank College of Life Science, Kyung Hee University , Yongin-si , Gyeonggi-do , Republic of Korea.
authors.forename: Sungeun
authors.initials: S
authors.lastname: Ahn
authors.affiliation: a Department of Oriental Medicine Biotechnology , Ginseng Bank College of Life Science, Kyung Hee University , Yongin-si , Gyeonggi-do , Republic of Korea.
authors.forename: Verónica
authors.initials: V
authors.lastname: Castro-Aceituno
authors.affiliation: b Graduate School of Biotechnology College of life science, Kyung Hee University , Yongin-si , Gyeonggi-do , Republic of Korea.
authors.forename: Zuly
authors.initials: Z
authors.lastname: Jiménez
authors.affiliation: b Graduate School of Biotechnology College of life science, Kyung Hee University , Yongin-si , Gyeonggi-do , Republic of Korea.
authors.forename: Shakina Yesmin
authors.initials: SY
authors.lastname: Simu
authors.affiliation: a Department of Oriental Medicine Biotechnology , Ginseng Bank College of Life Science, Kyung Hee University , Yongin-si , Gyeonggi-do , Republic of Korea.
authors.forename: Yeon Ju
authors.initials: YJ
authors.lastname: Kim
authors.affiliation: a Department of Oriental Medicine Biotechnology , Ginseng Bank College of Life Science, Kyung Hee University , Yongin-si , Gyeonggi-do , Republic of Korea.|b Graduate School of Biotechnology College of life science, Kyung Hee University , Yongin-si , Gyeonggi-do , Republic of Korea.
authors.forename: Deok Chun
authors.initials: DC
authors.lastname: Yang
mesh_terms: D041721:3T3-L1 Cells; D000818:Animals; D001713:Biphenyl Compounds / Q000737:chemistry; D002470:Cell Survival / Q000187:drug effects; D060326:Chemistry Techniques, Synthetic; D006046:Gold / Q000737:chemistry* / Q000494:pharmacology; D006801:Humans; D008422:Materials Testing; D053768:Metal Nanoparticles; D051379:Mice; D036103:Nanotechnology; D009569:Nitric Oxide / Q000096:biosynthesis; D005894:Panax / Q000737:chemistry*; D010851:Picrates / Q000737:chemistry; D018515:Plant Leaves / Q000737:chemistry*; D012834:Silver / Q000737:chemistry* / Q000494:pharmacology
chemical_list: D001713:Biphenyl Compounds; D010851:Picrates; D009569:Nitric Oxide; D012834:Silver; D006046:Gold; C004931:1,1-diphenyl-2-picrylhydrazyl
keywords: Panax ginseng; anti-inflammation; anticancer; antioxidant; gold nanoparticles; silver nanoparticles