title: Comparison of the patterns of antibody recall responses to HIV-1 gp120 and hepatitis B surface antigen in immunized mice.
abstract: To date, we still lack an ideal strategy for designing envelope glycoprotein (Env) vaccines to elicit potent protective antibodies against HIV-1 infection. Since the human hepatitis B virus surface antigen (HBsAg) is representative of effective vaccines that can induce ideal humoral immune responses, knowledge of how it elicits antibody responses and T helper cells would be an useful reference for HIV vaccine development. We compared the characteristics of the HIV-1 Env gp120 trimer and HBsAg in antibody elicitation and induction of T follicular helper (Tfh) and memory B cells in immunized Balb/c mice. Using the strategy of protein prime-protein boost, we found that HIV-1 gp120 induced slower recall antibody responses but redundant non-specific IgG responses at early time after boosting compared to HBsAg. The higher frequency of PD-1hiCD4+T cells and Tfh cells that appeared at the early time point after gp120 boosting is likely to limit the development of memory B cells, memory T cells, and specific antibody recall responses. These findings regarding the different features of HIV envelope and HBsAg in T helper cell responses may provide a direction to improve HIV envelope immunogenicity.
authors.affiliation: Department of Microbiology, Harbin Medical University, Harbin, China.
authors.forename: Hao-Tong
authors.initials: HT
authors.lastname: Yu
authors.affiliation: Department of Microbiology, Harbin Medical University, Harbin, China; Heilongjiang Provincial Key Laboratory of Infection and Immunity, Key Laboratory of Pathogen Biology, Harbin, China; Wu Lien-Teh Institute, Harbin Medical University, Harbin, China.
authors.forename: Jia-Ye
authors.initials: JY
authors.lastname: Wang
authors.affiliation: Department of Microbiology, Harbin Medical University, Harbin, China.
authors.forename: Dan
authors.initials: D
authors.lastname: Tian
authors.affiliation: Department of Microbiology, Harbin Medical University, Harbin, China.
authors.forename: Ming-Xia
authors.initials: MX
authors.lastname: Wang
authors.affiliation: Department of Microbiology, Harbin Medical University, Harbin, China; Heilongjiang Provincial Key Laboratory of Infection and Immunity, Key Laboratory of Pathogen Biology, Harbin, China; Wu Lien-Teh Institute, Harbin Medical University, Harbin, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Li
authors.affiliation: Department of Microbiology, Harbin Medical University, Harbin, China.
authors.forename: Li
authors.initials: L
authors.lastname: Yuan
authors.affiliation: Department of Microbiology, Harbin Medical University, Harbin, China.
authors.forename: Wen-Jiang
authors.initials: WJ
authors.lastname: Chen
authors.affiliation: Department of Microbiology, Harbin Medical University, Harbin, China; Heilongjiang Provincial Key Laboratory of Infection and Immunity, Key Laboratory of Pathogen Biology, Harbin, China.
authors.forename: Di
authors.initials: D
authors.lastname: Li
authors.affiliation: Department of Microbiology, Harbin Medical University, Harbin, China; Heilongjiang Provincial Key Laboratory of Infection and Immunity, Key Laboratory of Pathogen Biology, Harbin, China; Wu Lien-Teh Institute, Harbin Medical University, Harbin, China. Electronic address: zhuangm@ems.hrbmu.edu.cn.
authors.forename: Min
authors.initials: M
authors.lastname: Zhuang
authors.affiliation: Department of Microbiology, Harbin Medical University, Harbin, China; Heilongjiang Provincial Key Laboratory of Infection and Immunity, Key Laboratory of Pathogen Biology, Harbin, China; Wu Lien-Teh Institute, Harbin Medical University, Harbin, China; Department of Parasitology, Harbin Medical University, Harbin, China. Electronic address: lingh@ems.hrbmu.edu.cn.
authors.forename: Hong
authors.initials: H
authors.lastname: Ling
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D000917:Antibody Formation; D001402:B-Lymphocytes / Q000276:immunology; D005260:Female; D015699:HIV Envelope Protein gp120 / Q000276:immunology*; D006514:Hepatitis B Surface Antigens / Q000276:immunology*; D008807:Mice, Inbred BALB C; D013601:T-Lymphocytes / Q000276:immunology
chemical_list: D000914:Antibodies, Viral; D015699:HIV Envelope Protein gp120; D006514:Hepatitis B Surface Antigens; C514793:gp120 protein, Human immunodeficiency virus 1
keywords: Envelope; HBsAg; HIV-1; Immune memory; PD-1; T follicular helper cells