title: Immunological Identification of Fungal Species.
abstract: Immunodetection is described in this chapter as a technique for producing specific antibodies for antigen detection of the major human fungal pathogens. In the case of Candida spp., heat-killed cells are used to immunize mice over a couple of weeks and then splenocytes are isolated and further fused with myelomas to easily propagate the antibodies produced in the mice. The resulting antibodies follow a purification process where antibody levels and concentrations are determined. Fungal cells are also lysed to obtain whole cell extracts as a prior step for identification of antigens using immunoprecipitation. Finally, this method permits the production of specific antibodies against fungi and the identification of the respective antigens in an in vivo model.
authors.affiliation: CCRI-Children's Cancer Research Institute, Vienna, Austria.|Labdia-Labordiagnostik GmbH, Vienna, Austria.|Max F. Perutz Laboratories, MFPL-Department of Medical Biochemistry, Medical University of Vienna, Campus Vienna Biocenter, Dr. Bohr-Gasse 9, Vienna, 1030, Austria.
authors.forename: Filomena
authors.initials: F
authors.lastname: Nogueira
authors.affiliation: Max F. Perutz Laboratories, MFPL-Department of Medical Biochemistry, Medical University of Vienna, Campus Vienna Biocenter, Dr. Bohr-Gasse 9, Vienna, 1030, Austria.
authors.forename: Fabian
authors.initials: F
authors.lastname: Istel
authors.affiliation: CCRI-Children's Cancer Research Institute, Vienna, Austria.|Labdia-Labordiagnostik GmbH, Vienna, Austria.|Max F. Perutz Laboratories, MFPL-Department of Medical Biochemistry, Medical University of Vienna, Campus Vienna Biocenter, Dr. Bohr-Gasse 9, Vienna, 1030, Austria.
authors.forename: Leonel
authors.initials: L
authors.lastname: Pereira
authors.affiliation: Max F. Perutz Laboratories, MFPL-Department of Medical Biochemistry, Medical University of Vienna, Campus Vienna Biocenter, Dr. Bohr-Gasse 9, Vienna, 1030, Austria.
authors.forename: Michael
authors.initials: M
authors.lastname: Tscherner
authors.affiliation: Max F. Perutz Laboratories, MFPL-Department of Medical Biochemistry, Medical University of Vienna, Campus Vienna Biocenter, Dr. Bohr-Gasse 9, Vienna, 1030, Austria. karl.kuchler@meduniwien.ac.at.
authors.forename: Karl
authors.initials: K
authors.lastname: Kuchler
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000302:isolation & purification; D000946:Antigens, Fungal / Q000276:immunology*; D002175:Candida / Q000276:immunology; D058365:Candidiasis, Invasive / Q000175:diagnosis* / Q000276:immunology; D062610:Fungal Polysaccharides / Q000276:immunology*; D006801:Humans; D006825:Hybridomas; D007118:Immunoassay; D008807:Mice, Inbred BALB C
chemical_list: D000911:Antibodies, Monoclonal; D000946:Antigens, Fungal; D062610:Fungal Polysaccharides
keywords: Antibodies; Antigens; Aspergillus; Candida; Cryptococcus; Fusarium; Immunodetection; Mucor; Rhizopus; Zygomycetes