title: The Extract ofChrysanthemum zawadskiivar.latilobumAmeliorates Collagen-Induced Arthritis in Mice.
abstract: Chrysanthemum zawadskiivar.latilobum(CZ) has been used for beverage or tea and also as folk medicine for the remedy of diverse inflammatory diseases. Nevertheless, the therapeutic effect of CZ on arthritis remains to be unknown. In this paper we aim to investigate the CZ's antiarthritic effect and mechanism of action both in vitro and in vivo. To assess CZ's antiarthritic effect, mouse models of type II collagen-induced arthritis (CIA) were used. Mice were used to gauge clinical arthritis index and histopathological changes. Reverse transcriptase-polymerase chain reaction (RT-PCR), western blotting, electrophoretic mobility shift assay (EMSA), and other biological methods were adopted to measure CZ's effect on arthritis and to understand the veiled mechanism of action. CZ greatly suppressed CIA, histopathological score, bone erosion, and osteoclast differentiation. Mechanistically, CZ inhibited the production of various inflammatory and arthritic mediators like inflammatory cytokines, matrix metalloproteinases (MMPs), and chemokines. Of note, CZ significantly suppressed the activation of the NF-κB pathway in vivo. CZ exerted an antiarthritic effect in CIA mice by curbing the production of crucial inflammatory and arthritis mediators. This study warrants further investigation of CZ for the use in human rheumatoid arthritis (RA).
authors.affiliation: Department of Immunology, College of Medicine, Konkuk University, Chungju 27478, Republic of Korea.
authors.forename: A-Ram
authors.identifier: 0000-0001-5237-6905
authors.initials: AR
authors.lastname: Kim
authors.affiliation: Department of Immunology, College of Medicine, Konkuk University, Chungju 27478, Republic of Korea.
authors.forename: Hyuk Soon
authors.initials: HS
authors.lastname: Kim
authors.affiliation: Department of Immunology, College of Medicine, Konkuk University, Chungju 27478, Republic of Korea.
authors.forename: Do Kyun
authors.initials: DK
authors.lastname: Kim
authors.affiliation: Department of Immunology, College of Medicine, Konkuk University, Chungju 27478, Republic of Korea.
authors.forename: Jun Ho
authors.initials: JH
authors.lastname: Lee
authors.affiliation: Green Cross Wellbeing Co., Ltd., Seongnam 13595, Republic of Korea.
authors.forename: Young Hyo
authors.initials: YH
authors.lastname: Yoo
authors.affiliation: Green Cross Wellbeing Co., Ltd., Seongnam 13595, Republic of Korea.
authors.forename: Jeom Yong
authors.identifier: 0000-0001-5432-7402
authors.initials: JY
authors.lastname: Kim
authors.affiliation: Green Cross Wellbeing Co., Ltd., Seongnam 13595, Republic of Korea.
authors.forename: Sun Kyu
authors.initials: SK
authors.lastname: Park
authors.affiliation: Department of Immunology, College of Medicine, Konkuk University, Chungju 27478, Republic of Korea.
authors.forename: Seung Taek
authors.initials: ST
authors.lastname: Nam
authors.affiliation: Department of Immunology, College of Medicine, Konkuk University, Chungju 27478, Republic of Korea.
authors.forename: Hyun Woo
authors.initials: HW
authors.lastname: Kim
authors.affiliation: Department of Immunology, College of Medicine, Konkuk University, Chungju 27478, Republic of Korea.
authors.forename: Young Hwan
authors.initials: YH
authors.lastname: Park
authors.affiliation: Department of Immunology, College of Medicine, Konkuk University, Chungju 27478, Republic of Korea.
authors.forename: Dajeong
authors.initials: D
authors.lastname: Lee
authors.affiliation: Department of Immunology, College of Medicine, Konkuk University, Chungju 27478, Republic of Korea.
authors.forename: Min Beom
authors.initials: MB
authors.lastname: Lee
authors.affiliation: College of Pharmacy, Duksung Women's University, Seoul 01369, Republic of Korea.
authors.forename: Young Mi
authors.identifier: 0000-0001-8230-7150
authors.initials: YM
authors.lastname: Kim
authors.affiliation: Department of Immunology, College of Medicine, Konkuk University, Chungju 27478, Republic of Korea.
authors.forename: Wahn Soo
authors.identifier: 0000-0002-0643-2395
authors.initials: WS
authors.lastname: Choi