title: Proteomics Screen Identifies Class I Rab11 Family Interacting Proteins as Key Regulators of Cytokinesis.
abstract: The 14-3-3 protein family orchestrates a complex network of molecular interactions that regulates various biological processes. Owing to their role in regulating the cell cycle and protein trafficking, 14-3-3 proteins are prevalent in human diseases such as cancer, diabetes, and neurodegeneration. 14-3-3 proteins are expressed in all eukaryotic cells, suggesting that they mediate their biological functions through evolutionarily conserved protein interactions. To identify these core 14-3-3 client proteins, we used an affinity-based proteomics approach to characterize and compare the human and Drosophila 14-3-3 interactomes. Using this approach, we identified a group of Rab11 effector proteins, termed class I Rab11 family interacting proteins (Rab11-FIPs), or Rip11 in Drosophila We found that 14-3-3 binds to Rip11 in a phospho-dependent manner to ensure its proper subcellular distribution during cell division. Our results indicate that Rip11 plays an essential role in the regulation of cytokinesis and that this function requires its association with 14-3-3 but not with Rab11. Together, our results suggest an evolutionarily conserved role for 14-3-3 in controlling Rip11-dependent protein transport during cytokinesis.
authors.affiliation: Institute for Research in Immunology and Cancer (IRIC), Université de Montréal, Montréal, Québec, Canada.
authors.forename: Carl
authors.initials: C
authors.lastname: Laflamme
authors.affiliation: Institute for Research in Immunology and Cancer (IRIC), Université de Montréal, Montréal, Québec, Canada.
authors.forename: Jacob A
authors.initials: JA
authors.lastname: Galan
authors.affiliation: Institute for Research in Immunology and Cancer (IRIC), Université de Montréal, Montréal, Québec, Canada.
authors.forename: Khaled
authors.initials: K
authors.lastname: Ben El Kadhi
authors.affiliation: Institute for Research in Immunology and Cancer (IRIC), Université de Montréal, Montréal, Québec, Canada.
authors.forename: Antoine
authors.initials: A
authors.lastname: Méant
authors.affiliation: Institute for Research in Immunology and Cancer (IRIC), Université de Montréal, Montréal, Québec, Canada.
authors.forename: Carlos
authors.initials: C
authors.lastname: Zeledon
authors.affiliation: Institute for Research in Immunology and Cancer (IRIC), Université de Montréal, Montréal, Québec, Canada.|Department of Pathology and Cell Biology, Faculty of Medicine, Université de Montréal, Montréal, Québec, Canada.
authors.forename: Sébastien
authors.initials: S
authors.lastname: Carréno
authors.affiliation: Institute for Research in Immunology and Cancer (IRIC), Université de Montréal, Montréal, Québec, Canada philippe.roux@umontreal.ca gregory.emery@umontreal.ca.|Department of Pathology and Cell Biology, Faculty of Medicine, Université de Montréal, Montréal, Québec, Canada.
authors.forename: Philippe P
authors.identifier: http://orcid.org/0000-0002-5962-0250
authors.initials: PP
authors.lastname: Roux
authors.affiliation: Institute for Research in Immunology and Cancer (IRIC), Université de Montréal, Montréal, Québec, Canada philippe.roux@umontreal.ca gregory.emery@umontreal.ca.|Department of Pathology and Cell Biology, Faculty of Medicine, Université de Montréal, Montréal, Québec, Canada.
authors.forename: Gregory
authors.identifier: http://orcid.org/0000-0002-6874-6875
authors.initials: G
authors.lastname: Emery
mesh_terms: D048948:14-3-3 Proteins / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D017124:Conserved Sequence; D048749:Cytokinesis; D004330:Drosophila; D019143:Evolution, Molecular; D057809:HEK293 Cells; D006801:Humans; D024101:Mitochondrial Proteins / Q000378:metabolism; D050505:Mutant Proteins / Q000378:metabolism; D010766:Phosphorylation; D011485:Protein Binding; D000072417:Protein Domains; D021381:Protein Transport; D040901:Proteomics / Q000379:methods*; D020691:rab GTP-Binding Proteins / Q000378:metabolism*; D000099193:rab11 GTP-Binding Proteins
chemical_list: D048948:14-3-3 Proteins; D024101:Mitochondrial Proteins; D050505:Mutant Proteins; D020691:rab GTP-Binding Proteins; D000099193:rab11 GTP-Binding Proteins
keywords: 14-3-3; cell division; cytokinesis; proteomics; vesicular trafficking