title: Propofol inhibits the growth and survival of gastric cancer cells in vitro through the upregulation of ING3.
abstract: Propofol is one of the most extensively used intravenous anesthetic agents and it can influence the biological behavior of gastric cancer. However, the underlying mechanism is poorly understood. In the present study, we found that propofol significantly inhibited cell proliferation, invasion and migration, and also promoted apoptosis in gastric carcinoma cell lines SGC-7901 and MGC-803, as detected using MTT, colony formation and flow cytometry assays, respectively. Moreover, propofol (10 and 20 µM) markedly upregulated the expression of inhibitor of growth 3 (ING3), which was lower in SGC-7901 and MGC-803 cells compared with that noted in normal human gastric epithelial cell lines GES-1 and HFE145. Furthermore, we transfected SGC-7901 and MGC-803 cells with ING3 overexpression vectors or ING3 small interference RNA (siING3), respectively, to assess the role of ING3 in propofol-induced antitumor activity. The siING3 transfection reversed the effects of propofol on the biological behavior of gastric cancer cells, while transfection of ING3 promoted the effects of propofol. In conclusion, our results indicate that propofol exerts an inhibitory effect on the growth and survival of gastric cancer cells by interfering with ING3 degradation.
authors.affiliation: Department of Anesthesiology, Affiliated Hospital of Logistics University of PAP, Tianjin 300162, P.R. China.
authors.forename: Cheng
authors.initials: C
authors.lastname: Yang
authors.affiliation: Department of Anesthesiology, Affiliated Hospital of Logistics University of PAP, Tianjin 300162, P.R. China.
authors.forename: Jie
authors.initials: J
authors.lastname: Gao
authors.affiliation: Department of Anesthesiology, Affiliated Hospital of Logistics University of PAP, Tianjin 300162, P.R. China.
authors.forename: Nuo
authors.initials: N
authors.lastname: Yan
authors.affiliation: Department of Anesthesiology, The Central Hospital of Enshi Autonomous Prefecture, Enshi, Hubei 445000, P.R. China.
authors.forename: Banglin
authors.initials: B
authors.lastname: Wu
authors.affiliation: Department of Anesthesiology, Affiliated Hospital of Logistics University of PAP, Tianjin 300162, P.R. China.
authors.forename: Yiqing
authors.initials: Y
authors.lastname: Ren
authors.affiliation: Department of Anesthesiology, Affiliated Hospital of Logistics University of PAP, Tianjin 300162, P.R. China.
authors.forename: Hui
authors.initials: H
authors.lastname: Li
authors.affiliation: Department of Anesthesiology, Affiliated Hospital of Logistics University of PAP, Tianjin 300162, P.R. China.
authors.forename: Jiamin
authors.initials: J
authors.lastname: Liang
mesh_terms: D017209:Apoptosis / Q000187:drug effects / Q000235:genetics; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects; D015536:Down-Regulation / Q000187:drug effects; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D018398:Homeodomain Proteins / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D015742:Propofol / Q000494:pharmacology*; D013274:Stomach Neoplasms / Q000235:genetics / Q000473:pathology*; D025521:Tumor Suppressor Proteins / Q000235:genetics* / Q000378:metabolism; D015854:Up-Regulation / Q000187:drug effects
chemical_list: D018398:Homeodomain Proteins; C459806:ING3 protein, human; D025521:Tumor Suppressor Proteins; D015742:Propofol