title: Hyperbaric oxygen increases tissue-plasminogen activator-induced thrombolysisin vitro, and reduces ischemic brain damage and edema in rats subjected to thromboembolic brain ischemia.
abstract: Recent data have shown that normobaric oxygen (NBO) increases the catalytic and thrombolytic efficiency of recombinant tissue plasminogen activator (rtPA)in vitro, and is as efficient as rtPA at restoring cerebral blood flow in rats subjected to thromboembolic brain ischemia. Therefore, in the present study, we studied the effects of hyperbaric oxygen (HBO) (i) on rtPA-induced thrombolysisin vitroand (ii) in rats subjected to thromboembolic middle cerebral artery occlusion-induced brain ischemia. HBO increases rtPA-induced thrombolysisin vitroto a greater extent than NBO; in addition, HBO treatment of 5-minute duration, but not of 25-minute duration, reduces brain damage and edemain vivo. In line with the facilitating effect of NBO on cerebral blood flow, our findings suggest that 5-minute HBO could have provided neuroprotection by promoting thrombolysis. The lack of effect of HBO exposure of longer duration is discussed.
authors.affiliation: Université de Caen Normandie - CNRS, UMR 6301 ISTCT, Equipe Cervoxy, Caen, France.
authors.forename: Laurent
authors.initials: L
authors.lastname: Chazalviel
authors.affiliation: Université de Caen Normandie, CURB, Caen, France.
authors.forename: Benoit
authors.initials: B
authors.lastname: Haelewyn
authors.affiliation: Université de Caen Normandie, Faculté de Médecine, France.
authors.forename: Mickael
authors.initials: M
authors.lastname: Degoulet
authors.affiliation: Hôpital d'Instruction des Armées (HIA) Sainte-Anne, Service de Médecine Hyperbare et Expertise Plongée (SMHEP), Toulon, France.
authors.forename: Jean-Eric
authors.initials: JE
authors.lastname: Blatteau
authors.affiliation: Institut de Recherche Biomédicale des Armées (IRBA), Equipe Résidente de Recherche Subaquatique Opérationnelle (ERRSO), Toulon, France.
authors.forename: Nicolas
authors.initials: N
authors.lastname: Vallée
authors.affiliation: Institut de Recherche Biomédicale des Armées (IRBA), Equipe Résidente de Recherche Subaquatique Opérationnelle (ERRSO), Toulon, France.
authors.forename: Jean-Jacques
authors.initials: JJ
authors.lastname: Risso
authors.affiliation: Université de Caen Normandie - INSERM, UMR 1075, Caen, France.
authors.forename: Stéphane
authors.initials: S
authors.lastname: Besnard
authors.affiliation: Université de Caen Normandie, Faculté de Médecine, France; Institut de Recherche Biomédicale des Armées (IRBA), Equipe Résidente de Recherche Subaquatique Opérationnelle (ERRSO), Toulon, France; Université Laval, Département d'Anesthésiologie, Québec, Canada.
authors.forename: Jacques H
authors.identifier: 0000-0002-6435-9819
authors.initials: JH
authors.lastname: Abraini
keywords: brain edema; brain ischemia; hyperbaric oxygen; in vitro; rat; stroke; thrombolysis; tissue-plasminogen activator