title: Mycobacterium smegmatis proteoliposome induce protection in a murine progressive pulmonary tuberculosis model.
abstract: Tuberculosis (TB) remains an important cause of mortality and morbidity. The TB vaccine, BCG, is not fully protective against the adult form of the disease and is unable to prevent its transmission although it is still useful against severe childhood TB. Hence, the search for new vaccines is of great interest. In a previous study, we have shown that proteoliposomes obtained from Mycobacterium smegmatis (PLMs) induced cross reactive humoral and cellular response against Mycobacterium tuberculosis (Mtb) antigens. With the objective to evaluate the protective capability of PLMs, a murine model of progressive pulmonary TB was used. Animals immunized with PLMs with and without alum (PLMs/PLMsAL respectively) showed protection compared to non-immunized animals. Mice immunized with PLMsAL induced similar protection as that of BCG. Animals immunized with BCG, PLMs and PLMsAL showed a significant decrease in tissue damage (percentage of pneumonic area/lung) compared to non-immunized animals, with a more prominent effect in BCG vaccinated mice. The protective effect of the administration of PLMs in mice supports its future evaluation as experimental vaccine candidate against Mtb.
authors.affiliation: Institute Finlay, La Habana, Cuba.
authors.forename: Yanely
authors.initials: Y
authors.lastname: Tirado
authors.affiliation: Institute Finlay, La Habana, Cuba.
authors.forename: Alina
authors.initials: A
authors.lastname: Puig
authors.affiliation: Institute Finlay, La Habana, Cuba.
authors.forename: Nadine
authors.initials: N
authors.lastname: Alvarez
authors.affiliation: Institute Finlay, La Habana, Cuba.
authors.forename: Reinier
authors.initials: R
authors.lastname: Borrero
authors.affiliation: Institute Finlay, La Habana, Cuba.
authors.forename: Alicia
authors.initials: A
authors.lastname: Aguilar
authors.affiliation: Institute Finlay, La Habana, Cuba.
authors.forename: Frank
authors.initials: F
authors.lastname: Camacho
authors.affiliation: Institute Finlay, La Habana, Cuba.
authors.forename: Fatima
authors.initials: F
authors.lastname: Reyes
authors.affiliation: Institute Finlay, La Habana, Cuba.
authors.forename: Sonsire
authors.initials: S
authors.lastname: Fernandez
authors.affiliation: Institute Finlay, La Habana, Cuba.
authors.forename: Jose Luis
authors.initials: JL
authors.lastname: Perez
authors.affiliation: Institute Finlay, La Habana, Cuba.
authors.forename: Reynaldo
authors.initials: R
authors.lastname: Acevedo
authors.affiliation: Experimental Pathology Section, Department of Pathology, National Institute of Medical Sciences and Nutrition "Salvador Zubiran", D.F. Mexico, Mexico.
authors.forename: Dulce
authors.initials: D
authors.lastname: Mata Espinoza
authors.affiliation: Experimental Pathology Section, Department of Pathology, National Institute of Medical Sciences and Nutrition "Salvador Zubiran", D.F. Mexico, Mexico.
authors.forename: Jorge Alberto Barrios
authors.initials: JA
authors.lastname: Payan
authors.affiliation: Institute Finlay, La Habana, Cuba.
authors.forename: Maria de Los A
authors.initials: ML
authors.lastname: Garcia
authors.affiliation: School of Health Sciences, Universiti Sains Malaysia, Kelantan, Malaysia.
authors.forename: Ramlah
authors.initials: R
authors.lastname: Kadir
authors.affiliation: School of Health Sciences, Universiti Sains Malaysia, Kelantan, Malaysia.
authors.forename: María E
authors.initials: ME
authors.lastname: Sarmiento
authors.affiliation: Experimental Pathology Section, Department of Pathology, National Institute of Medical Sciences and Nutrition "Salvador Zubiran", D.F. Mexico, Mexico.
authors.forename: Rogelio
authors.initials: R
authors.lastname: Hernandez-Pando
authors.affiliation: School of Health Sciences, Universiti Sains Malaysia, Kelantan, Malaysia; INFORMM, Universiti Sains Malaysia, Kelantan, Malaysia. Electronic address: norazmimn@usm.my.
authors.forename: Mohd-Nor
authors.initials: MN
authors.lastname: Norazmi
authors.affiliation: INFORMM, Universiti Sains Malaysia, Kelantan, Malaysia. Electronic address: ducmar13@gmail.com.
authors.forename: Armando
authors.initials: A
authors.lastname: Acosta
mesh_terms: D000276:Adjuvants, Immunologic; D000534:Alum Compounds; D000818:Animals; D001500:BCG Vaccine; D058491:Bacterial Load; D004195:Disease Models, Animal; D018450:Disease Progression; D008297:Male; D008807:Mice, Inbred BALB C; D020102:Mycobacterium smegmatis / Q000276:immunology*; D009169:Mycobacterium tuberculosis / Q000254:growth & development / Q000302:isolation & purification; D018410:Pneumonia, Bacterial / Q000382:microbiology / Q000473:pathology / Q000517:prevention & control; D011510:Proteolipids / Q000276:immunology*; D032581:Tuberculosis Vaccines; D014397:Tuberculosis, Pulmonary / Q000276:immunology / Q000382:microbiology / Q000473:pathology / Q000517:prevention & control*
chemical_list: D000276:Adjuvants, Immunologic; D000534:Alum Compounds; D001500:BCG Vaccine; D011510:Proteolipids; D032581:Tuberculosis Vaccines; C020485:proteoliposomes; C041524:aluminum sulfate
keywords: Challenge; Mycobacterium smegmatis; Proteoliposomes; Tuberculosis; Vaccines