title: Simultaneous determination of docosahexaenoic acid and eicosapentaenoic acid in common seafood using ultrasonic cell crusher extraction combined with gas chromatography.
abstract: An effective method for the simultaneous determination of docosahexaenoic acid and eicosapentaenoic acid in common seafood by gas chromatography was developed and validated. Total docosahexaenoic acid and eicosapentaenoic acid were extracted from seafood by ultrasonic cell crusher assisted extraction and methyl esterified for gas chromatography analysis in the presence of the internal standard. The linearity was good (r > 0.999) in 9.59 ∼ 479.5 μg/mL for docosahexaenoic acid and 9.56 ∼ 477.8 μg/mL for eicosapentaenoic acid. The intrarun and interrun precisions were both within 4.8 and 6.1% for the two analytes, while the accuracy was less than 5.8%. The developed method was applied for determination of docosahexaenoic acid and eicosapentaenoic acid in six kinds of seafood. The result showed the content of docosahexaenoic acid and eicosapentaenoic acid was all higher than 1 mg/g in yellow croaker, hairtail, venerupis philippinarum, mussel, and oyster. Our work may be helpful for dietary optimization and production of docosahexaenoic acid and eicosapentaenoic acid.
authors.affiliation: School of Pharmaceutical Sciences, Binzhou Medical University, Yantai, P. R. China.
authors.forename: Juanjuan
authors.initials: J
authors.lastname: Zhao
authors.affiliation: School of Pharmaceutical Sciences, Binzhou Medical University, Yantai, P. R. China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Ren
authors.affiliation: School of Pharmaceutical Sciences, Binzhou Medical University, Yantai, P. R. China.
authors.forename: Chen
authors.initials: C
authors.lastname: Yu
authors.affiliation: School of Pharmaceutical Sciences, Binzhou Medical University, Yantai, P. R. China.
authors.forename: Xiangming
authors.initials: X
authors.lastname: Chen
authors.affiliation: School of Pharmaceutical Sciences, Binzhou Medical University, Yantai, P. R. China.
authors.forename: Yanan
authors.initials: Y
authors.lastname: Shi
mesh_terms: D002849:Chromatography, Gas; D004281:Docosahexaenoic Acids / Q000032:analysis*; D015118:Eicosapentaenoic Acid / Q000032:analysis*; D005504:Food Analysis / Q000379:methods*; D017747:Seafood / Q000032:analysis*; D014465:Ultrasonics
chemical_list: D004281:Docosahexaenoic Acids; D015118:Eicosapentaenoic Acid
keywords: Docosahexaenoic acid; eicosapentaenoic acid; gas chromatography; seafood; ultrasonic cell crusher