title: [Analysis of the protein expression differences between colonized and infectious multidrug-resistant acinetobacter baumannii].
abstract: Objective:To analyze the protein expression differences between colonized multidrug-resistantacinetobacter baumannii(MDR-AB) and infectious MDR-AB using comparative proteomics and matrix assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF MS) and to explore the molecular characteristics of MDR-AB infections.Methods:A total of 59 MDR-AB strains including 23 of bloodstream infections MDR-AB and 36 of nasal, pharyngeal or environmental colonization MDR-AB were collected from Beijing Tongren Hospital between January 2008 and December 2011. MALDI-TOF MS cluster analysis was applied to identify the differences of protein expression between infection strains and colonization strains (including patient colonization and environmental colonization) of MDR-AB. Two-dimensional protein differences electrophoresis and MALDI-TOF-TOF MS were used to analyze the protein expression differences between bloodstream infection strain and nasal colonization strain isolated from the same patient.Results:ST92 was the main multiple locus sequence typing of 23 bloodstream infections MDR-AB. Cluster analysis showed that there were differences of protein fingerprint of MADLI-TOF MS between colonized and infectious MDR-AB. Proteomic differences were found between colonization strain and infection strain which were isolated from the same patient. And 9 major different protein spots were identified. They were trigger factor (score of identification was 10 218 and coverage rate was 44%), inosine-5'-monophosphate dehydrogenase (score of identification was 1 919 and coverage rate was 60%), phosphoglycerate kinase (score of identification was 947 and coverage rate was 49%), outer membrane protein 38 (score of identification was 572 and coverage rate was 23%) and so on.Conclusion:There were differences of protein expression between MDR-AB infection strains and colonization strains. And such different proteins could provide experimental evidence for molecular markers research of MDR-AB infections.
authors.affiliation: Department of Clinical Laboratory, Beijing Tongren Hospital, Capital Medical University, Beijing 100730, China.
authors.forename: W J
authors.initials: WJ
authors.lastname: Sui
authors.forename: M
authors.initials: M
authors.lastname: Wang
authors.forename: Y F
authors.initials: YF
authors.lastname: Huang
authors.forename: X X
authors.initials: XX
authors.lastname: Lu
mesh_terms: D000151:Acinetobacter Infections; D040981:Acinetobacter baumannii; D016000:Cluster Analysis; D024881:Drug Resistance, Bacterial; D018432:Drug Resistance, Multiple; D006761:Hospitals; D006801:Humans; D008565:Membrane Proteins; D040901:Proteomics; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D008565:Membrane Proteins