title: Achieving ultrasensitive in vivo detection of bone crack with polydopamine-capsulated surface-enhanced Raman nanoparticle.
abstract: The timely diagnosis and intervention of bone microdamage are essential for preventing its accumulation and further diminishing the risk of skeletal fracture. Although staining methods have been adopted to better depict the microdamage on bone, their clinical impacts are still limited because they are based on histological sections which are inherently destructive. Here, highly sensitive and non-invasive in vivo detection of bone crack can be achieved by surface-enhanced Raman scattering (SERS) technique using a carefully chosen polydopamine (PDA)-SERS nanoparticle tag. The bone crack can be specifically labeled by PDA-SERS tags taking advantage of high affinity of PDA towards calcium exposed on the damaged bone, and identified by an intense featured Raman signal both in vitro and in vivo benefiting from the surface-enhanced resonance Raman scattering effect. As a preliminary in vivo application, it is found that PDA-SERS tags present no serious adverse effect in mouse model and hold good biocompatibility. This work may help the development of SERS technique for ultrasensitive in vivo detection of bone crack in clinic.
authors.affiliation: State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China.
authors.forename: Chunhuan
authors.initials: C
authors.lastname: Jiang
authors.affiliation: State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China; University of Chinese Academy of Sciences, Beijing 100049, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China.
authors.forename: Jiawei
authors.initials: J
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Supramolecular Structure and Materials, Jilin University, Changchun 130012, China. Electronic address: weisong@jlu.edu.cn.
authors.forename: Wei
authors.initials: W
authors.lastname: Song
authors.affiliation: State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China. Electronic address: lehuilu@ciac.ac.cn.
authors.forename: Lehui
authors.initials: L
authors.lastname: Lu
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000032:analysis; D002118:Calcium / Q000032:analysis*; D003287:Contrast Media / Q000138:chemical synthesis*; D005260:Female; D050723:Fractures, Bone / Q000175:diagnosis* / Q000378:metabolism / Q000473:pathology; D007211:Indoles / Q000737:chemistry*; D051379:Mice; D008813:Mice, Inbred ICR; D053769:Nanocapsules / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013059:Spectrum Analysis, Raman / Q000379:methods*; D020349:Surface Plasmon Resonance / Q000379:methods; D013499:Surface Properties
chemical_list: D015415:Biomarkers; D003287:Contrast Media; D007211:Indoles; D053769:Nanocapsules; D011108:Polymers; C568283:polydopamine; D002118:Calcium
keywords: Bone crack; In vivo detection; Polydopamine; Surface-enhanced Raman scattering; Ultrasensitive