title: [THE BRAF MUTATION AND THE POSSIBILITIES OF UVEAL MELANOMA METASTASING PROGNOSTIC MARKERS IDENTIFICATION].
abstract: OBJECTIVE
  The aim is to assess the BRAF gene mutations in patients with posterior uveal melanoma.
  
  
  METHODS
  Retrospective analysis of the group of patients with malignant melanoma of the uvea, who were indicated to enucleation between 1.1 2015 to 1.3.2016. We analyzed stage of uveal melanoma, volume, cell type and BRAF gene mutations.
  
  
  RESULTS
  In clinical study of 20 patients after enucleation due to uveal melanoma at the Department of Ophthalmology in Bratislava, patient age was ranged from 22 to 89 years with a median of 62 years. In 14 patients (70 %) enucleation was the primary treatment and in 6 patients (30 %) enucleation was after irradiation (brachytherapy, Leksell gama knife, linear accelerator). In 17 cases (85 %) the mutation of the BRAF gene was negative and in 3 cases the sample was not assessable for the BRAF mutation.
  
  
  CONCLUSIONS
  BRAF gene mutation is confirmed by several studies found in malignant melanoma of the skin. The histopathology findings in our group did not confirmed our theory, that since the uveal melanoma itself has the similar origin as skin melanoma, should also contain a BRAF mutation.Key words: malignant melanoma of the uvea, mutation of the BRAF gene, chromosomal abnormalities as a prognostic factor.
authors.forename: K
authors.initials: K
authors.lastname: Horkovičová
authors.forename: J
authors.initials: J
authors.lastname: Markus
authors.forename: I
authors.initials: I
authors.lastname: Krčová
authors.forename: P
authors.initials: P
authors.lastname: Babál
authors.forename: D
authors.initials: D
authors.lastname: Kobzová
authors.forename: B
authors.initials: B
authors.lastname: Smolková
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000235:genetics*; D001918:Brachytherapy; D004252:DNA Mutational Analysis; D015353:Eye Enucleation; D005260:Female; D006801:Humans; D008297:Male; D008545:Melanoma / Q000235:genetics* / Q000473:pathology / Q000601:surgery; D008875:Middle Aged; D009154:Mutation; D009362:Neoplasm Metastasis / Q000235:genetics; D011379:Prognosis; D048493:Proto-Oncogene Proteins B-raf / Q000235:genetics*; D012189:Retrospective Studies; D012878:Skin Neoplasms / Q000235:genetics / Q000473:pathology; D014604:Uveal Neoplasms / Q000235:genetics* / Q000473:pathology / Q000601:surgery; D055815:Young Adult; D000096142:Cutaneous Malignant Melanoma; D000098943:Uveal Melanoma
chemical_list: D014408:Biomarkers, Tumor; C482119:BRAF protein, human; D048493:Proto-Oncogene Proteins B-raf