title: Region-specific effects of oestradiol on adipose-derived stem cell differentiation in post-menopausal women.
abstract: The goal of this study was to determine the effect of acute transdermal 17β-oestradiol (E2) on the adipogenic potential of subcutaneous adipose-derived stem cells (ASC) in post-menopausal women. Post-menopausal women (n = 11; mean age 57 ± 4.5 years) were treated for 2 weeks, in a randomized, cross-over design, with transdermal E2(0.15 mg) or placebo patches. Biopsies of abdominal (AB) and femoral (FEM) subcutaneous adipose tissue (SAT) were obtained after each treatment and mature adipocytes were analysed for cell size and ASC for their capacity for proliferation (growth rate), differentiation (triglyceride accumulation) and susceptibility to tumour necrosis factor alpha-induced apoptosis. Gene expression of oestrogen receptors α and β (ESR1 and ESR2), perilipin 1 and hormone-sensitive lipase (HSL), was also assessed. In FEM SAT, but not AB SAT, 2 weeks of E2significantly (P = 0.03) increased ASC differentiation and whole SAT HSL mRNA expression (P = 0.03) compared to placebo. These changes were not associated with mRNA expression of oestrogen receptors α and β, but HSL expression was significantly increased in FEM SAT with transdermal E2treatment. Adipose-derived stem cells proliferation and apoptosis did not change in either SAT depot after E2compared with placebo. Short-term E2appeared to increase the adipogenic potential of FEM, but not AB, SAT in post-menopausal women with possible implications for metabolic disease. Future studies are needed to determine longer term impact of E2on regional SAT accumulation in the context of positive energy imbalance.
authors.affiliation: Department of Nutrition, Colorado State University, Fort Collins, CO, USA.
authors.forename: Kimberly A
authors.initials: KA
authors.lastname: Cox-York
authors.affiliation: Department of Medicine, Division of Geriatric Medicine, University of Colorado Anschutz Medical Campus, Aurora, CO, USA.
authors.forename: Christopher B
authors.initials: CB
authors.lastname: Erickson
authors.affiliation: Department of Medicine, Division of Endocrinology, Metabolism and Diabetes, University of Colorado Anschutz Medical Campus, Aurora, CO, USA.|Denver Health and Hospital Authority, Denver, CO, USA.
authors.forename: Rocio I
authors.initials: RI
authors.lastname: Pereira
authors.affiliation: Department of Medicine, Division of Endocrinology, Metabolism and Diabetes, University of Colorado Anschutz Medical Campus, Aurora, CO, USA.|Denver Health and Hospital Authority, Denver, CO, USA.
authors.forename: Daniel H
authors.initials: DH
authors.lastname: Bessesen
authors.affiliation: Department of Medicine, Division of Geriatric Medicine, University of Colorado Anschutz Medical Campus, Aurora, CO, USA.
authors.forename: Rachael E
authors.initials: RE
authors.lastname: Van Pelt
mesh_terms: D017667:Adipocytes / Q000166:cytology / Q000187:drug effects; D050156:Adipogenesis / Q000187:drug effects; D017209:Apoptosis / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects; D048429:Cell Size / Q000187:drug effects; D004958:Estradiol / Q000494:pharmacology*; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D008875:Middle Aged; D009928:Organ Specificity / Q000187:drug effects*; D017698:Postmenopause / Q000502:physiology*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D013234:Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D050151:Subcutaneous Fat / Q000166:cytology*
chemical_list: D012333:RNA, Messenger; D004958:Estradiol
keywords: adipogenesis; adipose tissue; menopause; oestrogen; stem cells