title: Development and characterization of phosphatidylcholine nanovesicles, containing garlic extract, with antilisterial activity in milk.
abstract: Phospholipid nanovesicles were developed to improve the stability of garlic (Allium sativum L.) extract. Electron microscopy of liposomes revealed nanometric and spherical-shaped vesicles with a mean particle size of 174.6±17.3nm and polydispersity index of 0.26±0.02. The entrapment efficiency was 47.5±7.3% and the nanoliposomes had a zeta potential of -16.2±5.5mV. The antimicrobial activity of free and encapsulated garlic extract was evaluated against different strains of Listeria spp. in milk at 37°C for 24h. For free and encapsulated garlic extracts at 5% concentration, a decrease of 4log cycles in viable cell counts was observed at 10h, against four of the five strains of Listeria spp. tested. The results indicate that liposomes constitute a suitable system for encapsulation of unstable garlic active compounds and the encapsulation of garlic extract proves to be a promising technology for multiple applications, including antimicrobial agents.
authors.affiliation: Laboratório de Bioquímica e Microbiologia Aplicada, Departamento de Ciência de Alimentos, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil.
authors.forename: Cristian Mauricio Barreto
authors.initials: CM
authors.lastname: Pinilla
authors.affiliation: Laboratório de Bioquímica e Microbiologia Aplicada, Departamento de Ciência de Alimentos, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil.
authors.forename: Caciano Pelayo Zapata
authors.initials: CP
authors.lastname: Noreña
authors.affiliation: Laboratório de Bioquímica e Microbiologia Aplicada, Departamento de Ciência de Alimentos, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil. Electronic address: abrand@ufrgs.br.
authors.forename: Adriano
authors.initials: A
authors.lastname: Brandelli
mesh_terms: D000818:Animals; D000890:Anti-Infective Agents / Q000494:pharmacology*; D002417:Cattle; D005737:Garlic / Q000737:chemistry*; D008081:Liposomes / Q000737:chemistry*; D008089:Listeria monocytogenes / Q000187:drug effects* / Q000254:growth & development*; D008892:Milk / Q000737:chemistry*; D053758:Nanoparticles / Q000008:administration & dosage / Q000737:chemistry*; D010713:Phosphatidylcholines / Q000737:chemistry; D010936:Plant Extracts / Q000494:pharmacology*
chemical_list: D000890:Anti-Infective Agents; D008081:Liposomes; D010713:Phosphatidylcholines; D010936:Plant Extracts
keywords: Allicin; Antimicrobial; Garlic extract; Listeria; Nanoparticle