title: Overexpression of heat shock GroEL stress protein in leptospiral biofilm.
abstract: Leptospira is the causative agent of leptospirosis, which is an emerging zoonotic disease. Recent studies on Leptospira have demonstrated biofilm formation on abiotic surfaces. The protein expressed in the biofilm was investigated by using SDS-PAGE and immunoblotting in combination with MALDI-TOF mass spectrometry. The proteins expressed in Leptospira biofilm and planktonic cells was analyzed and compared. Among these proteins, one (60 kDa) was found to overexpress in biofilm as compared to the planktonic cells. MALDI-TOF analysis identified this protein as stress and heat shock chaperone GroEL. Our findings demonstrate that GroEL is associated with Leptospira biofilm. GroEL is conserved, highly immunogenic and a prominent stress response protein in pathogenic Leptospira spp., which may have clinical relevance.
authors.affiliation: Regional Medical Research Centre (ICMR), WHO Collaborating Centre for Diagnosis, Reference, Research and Training in Leptospirosis, Port Blair 744101, Andaman and Nicobar Islands, India.
authors.forename: K
authors.initials: K
authors.lastname: Vinod Kumar
authors.affiliation: Regional Medical Research Centre (ICMR), WHO Collaborating Centre for Diagnosis, Reference, Research and Training in Leptospirosis, Port Blair 744101, Andaman and Nicobar Islands, India.
authors.forename: Chandan
authors.initials: C
authors.lastname: Lall
authors.affiliation: Regional Medical Research Centre (ICMR), WHO Collaborating Centre for Diagnosis, Reference, Research and Training in Leptospirosis, Port Blair 744101, Andaman and Nicobar Islands, India.
authors.forename: R
authors.initials: R
authors.lastname: Vimal Raj
authors.affiliation: National Hub for Healthcare Instrumentation Development (NHHID), Centre for Biotechnology, Anna University, Chennai 600 025, India.
authors.forename: K
authors.initials: K
authors.lastname: Vedhagiri
authors.affiliation: Regional Medical Research Centre (ICMR), WHO Collaborating Centre for Diagnosis, Reference, Research and Training in Leptospirosis, Port Blair 744101, Andaman and Nicobar Islands, India.
authors.forename: C
authors.initials: C
authors.lastname: Kartick
authors.affiliation: Regional Medical Research Centre (ICMR), WHO Collaborating Centre for Diagnosis, Reference, Research and Training in Leptospirosis, Port Blair 744101, Andaman and Nicobar Islands, India.
authors.forename: P
authors.initials: P
authors.lastname: Surya
authors.affiliation: Bharathidasan University, Department of Microbiology, School of Life Sciences, Tiruchirappalli 620 024, India.
authors.forename: K
authors.initials: K
authors.lastname: Natarajaseenivasan
authors.affiliation: Regional Medical Research Centre (ICMR), WHO Collaborating Centre for Diagnosis, Reference, Research and Training in Leptospirosis, Port Blair 744101, Andaman and Nicobar Islands, India. Electronic address: pblicmr@sancharnet.in.
authors.forename: P
authors.initials: P
authors.lastname: Vijayachari
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D018441:Biofilms / Q000254:growth & development*; D018834:Chaperonin 60 / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D015870:Gene Expression; D015151:Immunoblotting; D007919:Leptospira / Q000235:genetics* / Q000254:growth & development* / Q000378:metabolism; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D001426:Bacterial Proteins; D018834:Chaperonin 60
keywords: Biofilm; GroEL; Hsp; Leptospira; Planktonic