title: Hormonal profiling: Development of a simple method to extract and quantify phytohormones in complex matrices by UHPLC-MS/MS.
abstract: Plant growth regulators (PGRs) are very different chemical compounds that play essential roles in plant development and the regulation of physiological processes. They exert their functions by a mechanism called cross-talk (involving either synergistic or antagonistic actions) thus; it is for great interest to study as many PGRs as possible to obtain accurate information about plant status. Much effort has been applied to develop methods capable of analyze large numbers of these compounds but frequently excluding some chemical families or important PGRs within each family. In addition, most of the methods are specially designed for matrices easy to work with. Therefore, we wanted to develop a method which achieved the requirements lacking in the literature and also being fast and reliable. Here we present a simple, fast and robust method for the extraction and quantification of 20 different PGRs using UHPLC-MS/MS optimized in complex matrices.
authors.affiliation: Área de Fisiología Vegetal. Departamento de Biología de Organismos y Sistemas, Universidad de Oviedo, Catedrático Rodrigo Uría, 33006 Oviedo, Spain. Electronic address: delatorre85@gmail.com.
authors.forename: Carolina
authors.initials: C
authors.lastname: Delatorre
authors.affiliation: Área de Fisiología Vegetal. Departamento de Biología de Organismos y Sistemas, Universidad de Oviedo, Catedrático Rodrigo Uría, 33006 Oviedo, Spain.
authors.forename: Ana
authors.initials: A
authors.lastname: Rodríguez
authors.affiliation: Servicio Regional de Investigación y Desarrollo Agroalimentario de Asturias, Finca Experimental "La Mata", 33820, Grado, Spain.
authors.forename: Lucía
authors.initials: L
authors.lastname: Rodríguez
authors.affiliation: Centro Tecnológico Forestal y de la Madera, Pumarabule s/n, 33936 Carbayín, Asturias, Spain.
authors.forename: Juan P
authors.initials: JP
authors.lastname: Majada
authors.affiliation: Laboratorio de Biotecnología Agroforestal, Escuela Politécnica de Mieres, Universidad de Oviedo, Gonzalo Gutiérrez Quirós, 33600 Mieres, Spain.
authors.forename: Ricardo J
authors.initials: RJ
authors.lastname: Ordás
authors.affiliation: Servicio Regional de Investigación y Desarrollo Agroalimentario de Asturias, Finca Experimental "La Mata", 33820, Grado, Spain.
authors.forename: Isabel
authors.initials: I
authors.lastname: Feito
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000191:economics / Q000379:methods*; D059625:Liquid-Liquid Extraction / Q000379:methods; D028223:Pinus / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry*; D010937:Plant Growth Regulators / Q000032:analysis* / Q000302:isolation & purification; D012997:Solvents / Q000737:chemistry; D053719:Tandem Mass Spectrometry / Q000191:economics / Q000379:methods*
chemical_list: D010936:Plant Extracts; D010937:Plant Growth Regulators; D012997:Solvents
keywords: Complex matrix; Pinus pinaster; Plant growth regulator; Tandem mass spectrometry; UHPLC