title: Characterization of a neutral recombinant xylanase from Thermoactinospora rubra YIM 77501T.
abstract: A xylanase gene (TrXyn10) from Thermoactinospora rubra YIM 77501Twas cloned and expressed in Escherichia coli. The amino acid sequence displayed 78% homology with Microbispora mesophila xylanase (WP_062413927.1). The recombinant xylanase (TrXyn10), with MW 46.1 kDa, could hydrolyse beechwood, birchwood and oatspelt xylan. Based on the sequence, enzymatic properties and tertiary structure of the protein, TrXyn10 belongs to glycoside hydrolase family 10 (GH10). The optimal pH and temperature for the recombinant enzyme were determined to be 7.0 and 55 °C, respectively. TrXyn10 was stable over a wide pH range, and it retained more than 45% of the total activity at pH 6.0-12.0 for 12 h. In addition, the activity was greatly promoted, by approximately 200% of the initial activity, after incubation at pH 6.0 and 7.0 for 12 h. Based on enzymatic properties and product analysis, we showed that TrXyn10 is a neutral endoxylanase.
authors.affiliation: Yunnan Institute of Microbiology, Yunnan University, 650091, Kunming, People's Republic of China.
authors.forename: Yi-Rui
authors.initials: YR
authors.lastname: Yin
authors.affiliation: Yunnan Institute of Microbiology, Yunnan University, 650091, Kunming, People's Republic of China.
authors.forename: Qing-Wen
authors.initials: QW
authors.lastname: Hu
authors.affiliation: State Key Laboratory of Biocontrol and Guangdong Provincial Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat-Sen University, 510275, Guangzhou, People's Republic of China.
authors.forename: Wen-Dong
authors.initials: WD
authors.lastname: Xian
authors.affiliation: Key Laboratory of Biopesticide and Chemical Biology, Ministry of Education, and School of Life Sciences, Fujian Agriculture and Forestry University, 350002, Fuzhou, People's Republic of China.
authors.forename: Feng
authors.initials: F
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Biocontrol and Guangdong Provincial Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat-Sen University, 510275, Guangzhou, People's Republic of China.
authors.forename: En-Min
authors.initials: EM
authors.lastname: Zhou
authors.affiliation: Yunnan Institute of Microbiology, Yunnan University, 650091, Kunming, People's Republic of China.|College of Life Sciences and Technology, Xinxiang Medical University, 453003, Xinxiang, People's Republic of China.
authors.forename: Hong
authors.initials: H
authors.lastname: Ming
authors.affiliation: State Key Laboratory of Biocontrol and Guangdong Provincial Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat-Sen University, 510275, Guangzhou, People's Republic of China.
authors.forename: Min
authors.initials: M
authors.lastname: Xiao
authors.affiliation: Yunnan Institute of Microbiology, Yunnan University, 650091, Kunming, People's Republic of China. xyzhi@ynu.edu.cn.
authors.forename: Xiao-Yang
authors.initials: XY
authors.lastname: Zhi
authors.affiliation: Yunnan Institute of Microbiology, Yunnan University, 650091, Kunming, People's Republic of China. liwenjun3@mail.sysu.edu.cn.|State Key Laboratory of Biocontrol and Guangdong Provincial Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat-Sen University, 510275, Guangzhou, People's Republic of China. liwenjun3@mail.sysu.edu.cn.
authors.forename: Wen-Jun
authors.initials: WJ
authors.lastname: Li
mesh_terms: D039903:Actinobacteria / Q000201:enzymology* / Q000235:genetics; D000595:Amino Acid Sequence; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000235:genetics; D043364:Endo-1,4-beta Xylanases / Q000235:genetics; D004789:Enzyme Activation; D004795:Enzyme Stability; D004926:Escherichia coli / Q000235:genetics; D006026:Glycoside Hydrolases; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D014990:Xylans / Q000378:metabolism; D014995:Xylosidases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*
chemical_list: D004269:DNA, Bacterial; D011994:Recombinant Proteins; D014990:Xylans; D006026:Glycoside Hydrolases; D014995:Xylosidases; D043364:Endo-1,4-beta Xylanases
keywords: Acid tolerant; Alkali tolerant; Thermoactinospora rubra YIM 77501T; Xylanase