title: The relationship betweenNQO1C609T andCATC-262Tgenetic polymorphisms and the risk of age-related cataracts.
abstract: Cataract is multi-factorial eye disease identified by the disturbance of the transparent ocular lens. There is significant evidence suggesting oxidative damage as a major cause of initiation and progression of numerous diseases including cataracts. NAD(P)H:quinone oxidoreductase 1 (NQO1; OMIM: 125860) and catalase (CAT, OMIM: 115500) are antioxidant enzymes that prevent cells from oxidative stress. The aim of the present study was to investigate the association betweenNQO1 C609T(Pro189Ser, rs1800566) andCATpromoterC-262T(rs1001179) genetic polymorphisms and the susceptibility to cataracts. A case-control study including 190 cataracts cases and 190 healthy subjects was carried out. Genotype distributions ofNQO1andCATpolymorphisms were examined using polymerase chain reactions and a restriction fragment length polymorphism (PCR-RFLP) approach to investigate the possible role of these polymorphisms as risk factors in the development of cataracts. Variant CT heterozygous and TT genotypes of theNQO1 C609Tpolymorphism were found to be associated with an increased risk of cataracts (CTvsCC, OR=1.61, 95%CI: 1.02-2.52, P=0.038), (CT/TTvsCC, OR=1.56, 95%CI: 1.02-2.4, P=0.040). In addition, compared to indoor work places and the CC genotype ofNQO1,outdoor work places and CT/TT genotypes ofNQO1were found to increase the risk of age-related cataracts (OR=2.75, 95%CI: 1.20-6.33, P=0.017). The analysis did not reveal, however, any statistically significant (P>0.05) difference betweenCAT C-262Tpolymorphism and the risk of cataracts.
authors.affiliation: Department of Biology, College of Sciences, Shiraz University, Shiraz, Iran.
authors.forename: Narjes
authors.initials: N
authors.lastname: Zarei
authors.affiliation: Department of Biology, College of Sciences, Shiraz University, Shiraz, Iran.
authors.forename: Iraj
authors.initials: I
authors.lastname: Saadat
authors.affiliation: Department of Ophthalmology, Shiraz University of Medical Sciences, Shiraz, Iran.
authors.forename: Majid
authors.initials: M
authors.lastname: Farvardin-Jahromi
keywords: CAT; Cataract; Genetic polymorphism; NQO1