title: Evaluation of the production of exopolysaccharides by two strains of the thermophilic bacterium Rhodothermus marinus.
abstract: The thermophile Rhodothermus marinus produces extracellular polysaccharides (EPSs) that forms a distinct cellular capsule. Here, the first data on EPS production in strains DSM4252Tand MAT493 are reported and compared. Cultures of both strains, supplemented with either glucose, sucrose, lactose or maltose showed that the EPS were produced both in the exponential and stationary growth phase and that production in the exponential phase was boosted by maltose supplementation, while stationary phase production was boosted by lactose. The latter was higher, resulting in 8.8 (DSM4252T) and 13.7mg EPS/g cell dry weight (MAT493) in cultures in marine broth supplemented with 10g/L lactose. The EPSs were heteropolymeric with an average molecular weight of 8×104Da and different monosaccharides, including arabinose and xylose. FT-IR spectroscopy revealed presence of hydroxyl, carboxyl, N-acetyl, amine, and sulfate ester groups, showing that R. marinus produces unusual sulfated EPS with high arabinose and xylose content.
authors.affiliation: Department of Biotechnology, Center for Chemistry and Chemical Engineering, Lund University, P.O. Box 124, 22100 Lund, Sweden. Electronic address: Roya.Rezaei@biotek.lu.se.
authors.forename: Roya R R
authors.initials: RR
authors.lastname: Sardari
authors.affiliation: Biochemistryand Structural Biology, Department of Chemistry, Lund University, P.O. Box 124, 22100 Lund, Sweden.
authors.forename: Evelina
authors.initials: E
authors.lastname: Kulcinskaja
authors.affiliation: Department of Biotechnology, Center for Chemistry and Chemical Engineering, Lund University, P.O. Box 124, 22100 Lund, Sweden.
authors.forename: Emanuel Y C
authors.initials: EY
authors.lastname: Ron
authors.affiliation: Matís ohf, Vinlandsleid 12, Reykjavik 113, Iceland.
authors.forename: Snædís
authors.initials: S
authors.lastname: Björnsdóttir
authors.affiliation: Matís ohf, Vinlandsleid 12, Reykjavik 113, Iceland.
authors.forename: Ólafur H
authors.initials: ÓH
authors.lastname: Friðjónsson
authors.affiliation: Matís ohf, Vinlandsleid 12, Reykjavik 113, Iceland; Faculty of Life and Environmental Sciences, University of Iceland, Askja, Reykjavik IS-101, Iceland.
authors.forename: Guðmundur Óli
authors.initials: GÓ
authors.lastname: Hreggviðsson
authors.affiliation: Department of Biotechnology, Center for Chemistry and Chemical Engineering, Lund University, P.O. Box 124, 22100 Lund, Sweden.
authors.forename: Eva Nordberg
authors.initials: EN
authors.lastname: Karlsson
mesh_terms: D000606:Amino Sugars / Q000737:chemistry; D001089:Arabinose / Q000737:chemistry; D005947:Glucose / Q000378:metabolism; D007785:Lactose / Q000378:metabolism; D008320:Maltose / Q000378:metabolism; D011135:Polysaccharides, Bacterial / Q000096:biosynthesis* / Q000737:chemistry / Q000302:isolation & purification; D045245:Rhodothermus / Q000737:chemistry / Q000145:classification / Q000378:metabolism*; D013395:Sucrose / Q000378:metabolism; D014574:Uronic Acids / Q000737:chemistry; D014994:Xylose / Q000737:chemistry
chemical_list: D000606:Amino Sugars; D011135:Polysaccharides, Bacterial; D014574:Uronic Acids; D013395:Sucrose; D008320:Maltose; D014994:Xylose; D001089:Arabinose; D005947:Glucose; D007785:Lactose
keywords: EPS; Exopolysaccharide; Heteropolymer; Rhodothermus marinus