title: Dose-dependent effect of glucose on GLP-1 secretion involves sweet taste receptor in isolated perfused rat ileum [Rapid Communication].
abstract: Luminal glucose is an important stimulus for glucagon-like peptide 1 (GLP-1) secretion from intestinal endocrine cells. However, the effects of luminal glucose concentration on GLP-1 secretion remain unknown. In this study, we investigated the effect of luminal glucose concentrations (3.5, 5, 10, 15, and 20 mmol/L) on GLP-1 secretion from isolated perfused rat ileum. Results showed that the perfusate glucose concentration dose-dependently stimulated GLP-1 secretion from isolated perfused rat ileum, which was eliminated by the sweet taste receptor inhibitor gurmarin (30 μg/mL), but not inhibited by phloridzin (1 mmol/L), a Na+-coupled glucose transporters inhibitor. We conclude that luminal glucose induced GLP-1 secretion from perfused rat ileum in a concentration-dependent manner. This secretion was mediated by sweet taste receptor transducing signal for GLP-1 release on the gut of rat.
authors.affiliation: Institute of Pathogen Biology and Immunology, Department of Biochemistry, North University of Hebei, Zhangjiakou 075000, PR China.
authors.forename: Zhiwei
authors.initials: Z
authors.lastname: Xu
authors.forename: Wendong
authors.initials: W
authors.lastname: Wang
authors.forename: Xiaofeng
authors.initials: X
authors.lastname: Nian
authors.forename: Guiqin
authors.initials: G
authors.lastname: Song
authors.forename: Xiaoyun
authors.initials: X
authors.lastname: Zhang
authors.forename: Hongyuan
authors.initials: H
authors.lastname: Xiao
authors.forename: Xiaobo
authors.initials: X
authors.lastname: Zhu
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D018819:Dipeptidyl Peptidase 4 / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D052216:Glucagon-Like Peptide 1 / Q000378:metabolism*; D005947:Glucose / Q000494:pharmacology*; D007082:Ileum / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D009924:Organ Culture Techniques; D010940:Plant Proteins / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D043562:Receptors, G-Protein-Coupled / Q000378:metabolism / Q000502:physiology*; D015398:Signal Transduction / Q000187:drug effects
chemical_list: D010940:Plant Proteins; D043562:Receptors, G-Protein-Coupled; C431926:taste receptors, type 1; C075206:Gurmarin protein, Gymnema sylvestre; D052216:Glucagon-Like Peptide 1; C000602659:DPP4 protein, rat; D018819:Dipeptidyl Peptidase 4; D005947:Glucose