title: Danggui Buxue Tang Attenuates Tubulointerstitial Fibrosis via Suppressing NLRP3 Inflammasome in a Rat Model of Unilateral Ureteral Obstruction.
abstract: Inflammation significantly contributes to the progression of chronic kidney disease (CKD). This study aimed to characterize Danggui Buxue Tang (DBT) renoprotection and relationship with NOD-like receptors family pyrin domain-containing 3 (NLRP3) inflammasome expression in rats with unilateral ureteral obstruction (UUO). Sprague-Dawley rats were subjected to UUO and randomly assigned to untreated UUO, enalapril-treated (10 mg/kg/day), and DBT-treated (9 g/kg/day) groups. Sham-operated rats served as controls, with 8 rats in each group. All rats were sacrificed for blood and renal specimen collection at 14 days after UUO. Untreated UUO rats exhibited azotemia, intense tubulointerstitial collagen deposition, upregulations of tubulointerstitial injury index, augmentation levels of collagen I (Col I),α-smooth muscle actin (α-SMA), NLRP3, apoptosis-associated speck-like protein containing a caspase recruitment domain (ASC), pro-caspase-1, caspase-1, IL-1β, and pro-IL-1β. DBT treatment significantly attenuated interstitial collagen deposition and tubulointerstitial injury, lowering Col I andα-SMA levels. Synchronous expressions of NLRP3, ASC, pro-caspase-1, caspase-1, pro-IL-1β, and IL-1βdecreased in renal tissue. In comparison to enalapril, DBT significantly reduced tubulointerstitial injury, interstitial collagen deposition, and expressions of Col I and IL-1β. Thus, DBT offers renoprotection in UUO rats, which was associated with suppressing NLRP3 inflammasome expression and following reduction of the secretion of cytokine IL-1β. The mechanisms of multitargets of traditional Chinese medicine can be better used for antifibrotic treatment.
authors.affiliation: Department of Nephrology, Chinese PLA General Hospital, Chinese PLA Institute of Nephrology, State Key Laboratory of Kidney Diseases, National Clinical Research Center for Kidney Diseases, Beijing 100853, China; Department of Nephrology, Henan Province Hospital of Chinese Medicine, Zhengzhou, Henan 450002, China.
authors.forename: Linna
authors.identifier: 0000-0001-5075-9224
authors.initials: L
authors.lastname: Wang
authors.affiliation: Department of Nephrology, Chinese PLA General Hospital, Chinese PLA Institute of Nephrology, State Key Laboratory of Kidney Diseases, National Clinical Research Center for Kidney Diseases, Beijing 100853, China; The First Affiliated Hospital of Henan Traditional Chinese Medicine University, Zhengzhou, Henan 450008, China.
authors.forename: Jiwei
authors.initials: J
authors.lastname: Ma
authors.affiliation: Department of Nephrology, Henan Province Hospital of Chinese Medicine, Zhengzhou, Henan 450002, China.
authors.forename: Cunxia
authors.initials: C
authors.lastname: Guo
authors.affiliation: Department of Radiology, Xiangya Hospital, Central South University, Changsha, Hunan 410008, China.
authors.forename: Changyong
authors.initials: C
authors.lastname: Chen
authors.affiliation: Department of Nephrology, Chinese PLA General Hospital, Chinese PLA Institute of Nephrology, State Key Laboratory of Kidney Diseases, National Clinical Research Center for Kidney Diseases, Beijing 100853, China.
authors.forename: Zhong
authors.initials: Z
authors.lastname: Yin
authors.affiliation: Department of Nephrology, Chinese PLA General Hospital, Chinese PLA Institute of Nephrology, State Key Laboratory of Kidney Diseases, National Clinical Research Center for Kidney Diseases, Beijing 100853, China.
authors.forename: Xueguang
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Department of Nephrology, Chinese PLA General Hospital, Chinese PLA Institute of Nephrology, State Key Laboratory of Kidney Diseases, National Clinical Research Center for Kidney Diseases, Beijing 100853, China.
authors.forename: Xiangmei
authors.identifier: 0000-0001-8774-6021
authors.initials: X
authors.lastname: Chen
mesh_terms: D000818:Animals; D015536:Down-Regulation / Q000187:drug effects / Q000276:immunology; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage*; D005355:Fibrosis; D058847:Inflammasomes / Q000276:immunology; D008297:Male; D000071199:NLR Family, Pyrin Domain-Containing 3 Protein / Q000276:immunology*; D009395:Nephritis, Interstitial / Q000188:drug therapy* / Q000276:immunology* / Q000473:pathology; D051381:Rats; D017207:Rats, Sprague-Dawley; D016896:Treatment Outcome; D014517:Ureteral Obstruction / Q000188:drug therapy* / Q000276:immunology* / Q000473:pathology
chemical_list: D004365:Drugs, Chinese Herbal; D058847:Inflammasomes; D000071199:NLR Family, Pyrin Domain-Containing 3 Protein; C573346:Nlrp3 protein, rat; C477279:danggui buxue decoction