title: Identification of potential genes for human ischemic cardiomyopathy based on RNA-Seq data.
abstract: Ischemic cardiomyopathy (ICM) is an important cause of heart failure, yet no ICM disease genes were stored in any public databases. Mutations of genes provided by RNA-Seq data could set a foundation for a variety of biological processes. This also made it possible to elucidate the mechanism and identify potential genes for ICM. In this paper, an integrated co-expression network was constructed using univariate and bivariate canonical correlation analysis for RNA-Seq data of human ICM samples. Three ICM-related modules were recognized after comparing between Pearson correlation coefficients of ICM samples and normal controls. Furthermore, 32 ICM potential genes were identified from ICM-related modules considering protein-protein interactions. Most of these genes were verified to be involved in ICM and diseases caused it by OMIM and literature. Our study could provide a novel perspective for potential gene identification and the pathogenesis for ICM and other complex diseases.
authors.affiliation: College of Bioinformatics Science and Technology, Harbin Medical University, Harbin, Heilongjiang, China.
authors.forename: Wan
authors.initials: W
authors.lastname: Li
authors.affiliation: College of Bioinformatics Science and Technology, Harbin Medical University, Harbin, Heilongjiang, China.
authors.forename: Liansheng
authors.initials: L
authors.lastname: Li
authors.affiliation: College of Bioinformatics Science and Technology, Harbin Medical University, Harbin, Heilongjiang, China.
authors.forename: Shiying
authors.initials: S
authors.lastname: Zhang
authors.affiliation: Department of internal medicine, Heilongjiang Commercial Hospital, Harbin, Heilongjiang, China.
authors.forename: Ce
authors.initials: C
authors.lastname: Zhang
authors.affiliation: College of Bioinformatics Science and Technology, Harbin Medical University, Harbin, Heilongjiang, China.
authors.forename: Hao
authors.initials: H
authors.lastname: Huang
authors.affiliation: College of Bioinformatics Science and Technology, Harbin Medical University, Harbin, Heilongjiang, China.
authors.forename: Yiran
authors.initials: Y
authors.lastname: Li
authors.affiliation: College of Bioinformatics Science and Technology, Harbin Medical University, Harbin, Heilongjiang, China.
authors.forename: Erqiang
authors.initials: E
authors.lastname: Hu
authors.affiliation: College of Bioinformatics Science and Technology, Harbin Medical University, Harbin, Heilongjiang, China.
authors.forename: Gui
authors.initials: G
authors.lastname: Deng
authors.affiliation: College of Bioinformatics Science and Technology, Harbin Medical University, Harbin, Heilongjiang, China.
authors.forename: Shanshan
authors.initials: S
authors.lastname: Guo
authors.affiliation: College of Bioinformatics Science and Technology, Harbin Medical University, Harbin, Heilongjiang, China.
authors.forename: Yahui
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Cardiology, the First Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang, China.
authors.forename: Weimin
authors.initials: W
authors.lastname: Li
authors.affiliation: College of Bioinformatics Science and Technology, Harbin Medical University, Harbin, Heilongjiang, China.
authors.forename: Lina
authors.initials: L
authors.lastname: Chen
mesh_terms: D009202:Cardiomyopathies / Q000175:diagnosis / Q000209:etiology / Q000235:genetics*; D016022:Case-Control Studies; D019295:Computational Biology; D030541:Databases, Genetic; D005786:Gene Expression Regulation; D053263:Gene Regulatory Networks; D056726:Genetic Association Studies; D005819:Genetic Markers; D020022:Genetic Predisposition to Disease; D006801:Humans; D017202:Myocardial Ischemia / Q000150:complications / Q000175:diagnosis / Q000235:genetics*; D010641:Phenotype; D060066:Protein Interaction Maps; D012313:RNA / Q000235:genetics*; D017423:Sequence Analysis, RNA; D018511:Systems Integration
chemical_list: D005819:Genetic Markers; D012313:RNA
keywords: Pathology Section; RNA-Seq; canonical correlation analysis; co-expression network; ischemic cardiomyopathy