title: BODIPY-conjugated chitosan nanoparticles as a fluorescent probe.
abstract: Recently, development of fluorescent nanoparticle-based probes for various bioimaging applications has attracted great attention. This work aims to develop a new type fluorescent nanoparticle conjugate and evaluate its cytotoxic effects on A549 and BEAS 2B cell lines. Throughout the study, ionically crosslinked chitosan nanoparticles (CNs) were conjugated with carboxylated 4,4-difluoro-4-bora-3a,4a-diaza-s-indacene (BODIPY-COOH). The results of conjugates (BODIPY-CNs) were investigated with regard to their physic-chemical, optical, cytotoxic properties and cellular internalization. The morphology of BODIPY-CNs was found to be spherical in shape and quite uniform having average diameter of 70.25 ± 11.99 nm. Cytotoxicty studies indicated that although BODIPY-COOH itself was quite toxic on both A549- and BEAS 2B-treated cells, CNs increased the cell viability of both cell lines via conjugation to BODIPY-COOH fluorescent molecule up to 67% for A549 and 74% for BEAS 2B cells. These results may suggest a possible utilization of the new fluorescent nanoparticle-based probe for bioimaging in biology and medicine.
authors.affiliation: a Biotechnology and Bioengineering Department.
authors.forename: Gizem
authors.initials: G
authors.lastname: Bor
authors.affiliation: b Chemistry Department and.
authors.forename: Muhammed
authors.initials: M
authors.lastname: Üçüncü
authors.affiliation: b Chemistry Department and.
authors.forename: Mustafa
authors.initials: M
authors.lastname: Emrullahoğlu
authors.affiliation: c Materials Science and Engineering Department, Izmir Institute of Technology , Izmir , Turkey.
authors.forename: Aysel
authors.initials: A
authors.lastname: Tomak
authors.affiliation: b Chemistry Department and.
authors.forename: Gülşah
authors.initials: G
authors.lastname: Şanlı-Mohamed
mesh_terms: D065567:Absorption, Physiological; D001980:Bronchi / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D002460:Cell Line; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D048271:Chitosan / Q000009:adverse effects / Q000737:chemistry / Q000378:metabolism*; D003952:Diagnostic Imaging / Q000009:adverse effects; D000067493:Dynamic Light Scattering; D005456:Fluorescent Dyes / Q000009:adverse effects / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D018625:Microscopy, Atomic Force; D018613:Microscopy, Confocal; D008855:Microscopy, Electron, Scanning; D053758:Nanoparticles / Q000009:adverse effects / Q000737:chemistry / Q000378:metabolism* / Q000648:ultrastructure; D010316:Particle Size; D011162:Porphobilinogen / Q000009:adverse effects / Q000031:analogs & derivatives* / Q000737:chemistry / Q000378:metabolism; D020545:Respiratory Mucosa / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: C538930:3-anilino difluoroboron dipyrromethene; D005456:Fluorescent Dyes; D011162:Porphobilinogen; D048271:Chitosan
keywords: A549 cells; BEAS 2B cells; BODIPY; Chitosan; cytotoxicity; nanoparticles