title: Characterization of the subcellular localization and nuclear import molecular mechanisms of herpes simplex virus 1 UL2.
abstract: As a crucial protein, the herpes simplex virus 1 (HSV-1) UL2 protein has been shown to take part in various stages of viral infection, nonetheless, its exact subcellular localization and transport molecular determinants are not well known thus far. In the present study, by using live cells fluorescent microscopy assay, UL2 tagged with enhanced yellow fluorescent protein was transiently expressed in live cells and showed a completely nuclear accumulation without the presence of other HSV-1 proteins. Moreover, the nuclear transport of UL2 was characterized to be assisted by multiple transport pathways through Ran-, importin α1-, α5-, α7-, β1- and transportin-1 cellular transport receptors. Consequently, these results will improve understanding of UL2-mediated biological functions in HSV-1 infection cycles.
authors.forename: Mingsheng
authors.initials: M
authors.lastname: Cai
authors.forename: Zebin
authors.initials: Z
authors.lastname: Huang
authors.forename: Zongmin
authors.initials: Z
authors.lastname: Liao
authors.forename: Tao
authors.initials: T
authors.lastname: Chen
authors.forename: Ping
authors.initials: P
authors.lastname: Wang
authors.forename: Si
authors.initials: S
authors.lastname: Jiang
authors.forename: Daixiong
authors.initials: D
authors.lastname: Chen
authors.forename: Tao
authors.initials: T
authors.lastname: Peng
authors.forename: Yun
authors.initials: Y
authors.lastname: Bian
authors.forename: Gengde
authors.initials: G
authors.lastname: Hong
authors.forename: Hang
authors.initials: H
authors.lastname: Yang
authors.forename: Zhancheng
authors.initials: Z
authors.lastname: Zeng
authors.forename: Xiaowei
authors.initials: X
authors.lastname: Li
authors.forename: Meili
authors.initials: M
authors.lastname: Li
mesh_terms: D021581:Active Transport, Cell Nucleus; D000818:Animals; D019556:COS Cells; D002467:Cell Nucleus / Q000378:metabolism; D002522:Chlorocebus aethiops; D057809:HEK293 Cells; D006561:Herpes Simplex / Q000503:physiopathology* / Q000821:virology*; D018259:Herpesvirus 1, Human / Q000502:physiology*; D006801:Humans; D013347:Subcellular Fractions; D014018:Tissue Distribution; D051981:Uracil-DNA Glycosidase / Q000235:genetics / Q000378:metabolism*; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism*; D028901:alpha Karyopherins / Q000378:metabolism; D028961:beta Karyopherins / Q000378:metabolism
chemical_list: D014764:Viral Proteins; D028901:alpha Karyopherins; D028961:beta Karyopherins; C087978:karyopherin alpha 2; C000623137:UL2 protein, HSV-1; D051981:Uracil-DNA Glycosidase