title: Gastroprotective and ulcer healing effects of hydroethanolic extract of leaves of Caryocar coriaceum: Mechanisms involved in the gastroprotective activity.
abstract: This work aimed to determine the chemical fingerprint of hydroethanolic extract of leaves of Caryocar coriaceum (HELCC) and the gastroprotective activity. The chemical fingerprint of HELCC was analyzed by HPLC-DAD, to quantify total phenols and flavonoids were carried out by Folin-Ciocalteu reagent and aluminum chloride assay, while in vitro antioxidant activity was evaluated by the DPPH method. The methods used to determine pharmacological activity were: gastroprotective screening test in classical models of induced acute and chronic gastric lesions and physical barrier test. Further assays were performed to demonstrate the involvement of NO, prostaglandins, ATP-dependent potassium channels, TRPV, noradrenergic α2 receptors, histamines, and opioids. The DPPH method demonstrated the antioxidant activity of the extract, in vitro, explained by the presence of polyphenols and flavonoids. Oral administration of the extract, previously dissolved in deionized water, at a dose of 100 mg/kg decreased the lesions induced by indomethacin, acidified ethanol, ethanol and acetic acid by 75.0, 72.8, 69.4 and 86.2% respectively. It was demonstrated that opioid receptors, α2-adrenergic receptors and primary afferent neurons sensitive to capsaicin were involved in the mechanism of gastric protection, in addition to the contribution of NO and prostaglandins. The results show that extract is a promising candidate for the treatment of gastric ulcers.
authors.affiliation: Chemical-Biology Department, Regional University of Cariri - URCA, Cel. Antonio Luis 1161, Crato, CE, Brazil.
authors.forename: Luis Jardelino
authors.initials: LJ
authors.lastname: de Lacerda Neto
authors.affiliation: Chemical-Biology Department, Regional University of Cariri - URCA, Cel. Antonio Luis 1161, Crato, CE, Brazil.
authors.forename: Andreza Guedes Barbosa
authors.initials: AG
authors.lastname: Ramos
authors.affiliation: Chemical-Biology Department, Regional University of Cariri - URCA, Cel. Antonio Luis 1161, Crato, CE, Brazil.
authors.forename: Valterlucio
authors.initials: V
authors.lastname: Santos Sales
authors.affiliation: Chemical-Biology Department, Regional University of Cariri - URCA, Cel. Antonio Luis 1161, Crato, CE, Brazil.
authors.forename: Severino Denicio Gonçalves
authors.initials: SD
authors.lastname: de Souza
authors.affiliation: Chemical-Biology Department, Regional University of Cariri - URCA, Cel. Antonio Luis 1161, Crato, CE, Brazil.
authors.forename: Antonia Thassya Lucas
authors.initials: AT
authors.lastname: Dos Santos
authors.affiliation: Chemical-Biology Department, Regional University of Cariri - URCA, Cel. Antonio Luis 1161, Crato, CE, Brazil.
authors.forename: Larissa Rolim
authors.initials: LR
authors.lastname: de Oliveira
authors.affiliation: Chemical-Biology Department, Regional University of Cariri - URCA, Cel. Antonio Luis 1161, Crato, CE, Brazil.
authors.forename: Marta Regina
authors.initials: MR
authors.lastname: Kerntopf
authors.affiliation: Chemical-Biology Department, Regional University of Cariri - URCA, Cel. Antonio Luis 1161, Crato, CE, Brazil.
authors.forename: Thais Rodrigues
authors.initials: TR
authors.lastname: de Albuquerque
authors.affiliation: Chemical-Biology Department, Regional University of Cariri - URCA, Cel. Antonio Luis 1161, Crato, CE, Brazil. Electronic address: hdmcoutinho@gmail.com.
authors.forename: Henrique Douglas Melo
authors.initials: HD
authors.lastname: Coutinho
authors.affiliation: Department of Physiology, Federal University of Sergipe, Campus Universitário Prof. Aloísio de Campos, S/N São Cristovão, 49100001, Aracaju, SE, Brazil.
authors.forename: Lucindo Jose
authors.initials: LJ
authors.lastname: Quintans-Júnior
authors.affiliation: Department of Physiology and Pharmacology, Federal University of Pernambuco - UFPE, Av. Prof. Moraes Rego, S/N, Recife, PE, Brazil.
authors.forename: Almir Gonçalves
authors.initials: AG
authors.lastname: Wanderley
authors.affiliation: Chemical-Biology Department, Regional University of Cariri - URCA, Cel. Antonio Luis 1161, Crato, CE, Brazil. Electronic address: Irwin.alencar@urca.br.
authors.forename: Irwin Rose Alencar
authors.initials: IR
authors.lastname: de Menezes
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D002211:Capsaicin / Q000031:analogs & derivatives / Q000494:pharmacology / Q000627:therapeutic use; D002851:Chromatography, High Pressure Liquid; D002908:Chronic Disease; D004195:Disease Models, Animal; D020448:Ericales / Q000737:chemistry*; D000431:Ethanol / Q000737:chemistry*; D005260:Female; D005419:Flavonoids / Q000032:analysis; D005769:Gastrointestinal Motility; D005905:Glyburide / Q000494:pharmacology / Q000627:therapeutic use; D006632:Histamine / Q000494:pharmacology / Q000627:therapeutic use; D007213:Indomethacin; D008297:Male; D051379:Mice; D009093:Mucus / Q000187:drug effects; D019331:NG-Nitroarginine Methyl Ester / Q000494:pharmacology / Q000627:therapeutic use; D009270:Naloxone / Q000494:pharmacology / Q000627:therapeutic use; D010636:Phenols / Q000032:analysis; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology / Q000627:therapeutic use*; D018515:Plant Leaves / Q000737:chemistry*; D020011:Protective Agents / Q000627:therapeutic use*; D013276:Stomach Ulcer / Q000188:drug therapy* / Q000473:pathology; D014867:Water / Q000737:chemistry*; D015016:Yohimbine / Q000494:pharmacology / Q000627:therapeutic use
chemical_list: D000975:Antioxidants; D005419:Flavonoids; D010636:Phenols; D010936:Plant Extracts; D020011:Protective Agents; D014867:Water; D015016:Yohimbine; D009270:Naloxone; D000431:Ethanol; D006632:Histamine; C071423:capsazepine; D002211:Capsaicin; D005905:Glyburide; D019331:NG-Nitroarginine Methyl Ester; D007213:Indomethacin
keywords: Antioxidant; Caryocar coriaceum; Caryocaraceae; Chemcial profile; Gastroprotection; Mechanism of actions