title: Tips on ligand immobilization and kinetic study using surface plasmon resonance.
abstract: Surface plasmon resonance (SPR) technique offers a robust label-free approach applicable in various investigations including binding affinity, specificity and kinetics of biological macromolecules (e.g., peptides, proteins and nucleotidase) and small molecules. SPR provides extremely important data on the kinetics and affinity of substances examined, through which bio-specific interaction(s) can be established by the analysis of adsorption of analyteonto the immobilized ligand(s) on a sensor-based analytical system. Due to SPR wide applications in biomedical laboratories, the aim of this editorial is to highlight the importance of SPR in affinity kinetics and ligand immobilization.
authors.affiliation: Research Center for Pharmaceutical Nanotechnology, Tabriz University of Medical Sciences, Tabriz, Iran.
authors.forename: Jafar
authors.initials: J
authors.lastname: Ezzati Nazhad Dolatabadi
authors.affiliation: Department of Analytical Chemistry, University of Valencia, Valencia, Spain.
authors.forename: Miguel
authors.initials: M
authors.lastname: de la Guardia
keywords: Analyte; Kinetic study; Ligand immobilization; Surface plasmon resonance