title: [Effects of transthyretin on biological behavior of retinal pigment epithelial cells and retinal microvascular epithelial cells].
abstract: Objective:To explore the effects of transthyretin (TTR) on biological behavior of retinal pigment epithelial cells (RPECs) and retinal microvascular epithelial cells (RMVECs).Methods:RPECs were cultured with exogenous TTR to explore the effect of TTR on the proliferation of RPECs. The expression of TTR of RPECs was silenced by TTR specific small interfering RNA and the expression of TTR was detected by using Western blotting to identify the efficacy of TTR silence. The level of vascular endothelial growth factor (VEGF) massage RNA was detected by using RT-PCR to identify the interaction between VEGF and TTR. The different proliferation and migration abilities of RMVECs with different expressions of TTR were measured by transwell system.Results:MTT assay showed that RPECs with 0 μmol/L TTR glowed faster than with 4 μmol/L TTR (t=18.08,P<0.0001). The expression level of TTR was decreased in the small interfering RNA group as compared with the negative control group (P<0.05). RT-PCR assay showed no differential expression of VEGF after the silencing of TTR (P>0.05). The transwell assay showed RMVECs with the silence of TTR proliferated more slowly than RMVECs without the treatment (t=4.901,P=0.0012), and also migrated more slowly (t=4.213,P=0.0029).Conclusions:TTR can inhibit the proliferation of RPECs and promote the proliferation and migration of RMVECs without the help of VEGF, the mechanism of which may be worth further study.(Chin J Ophthalmol, 2016, 52: 856-860).
authors.affiliation: Department of Ophthalmology, Wuxi People's Hospital, Nanjing Medical University, Wuxi 214023, Jiangsu Province, China.
authors.forename: M
authors.initials: M
authors.lastname: Zhuang
authors.forename: J
authors.initials: J
authors.lastname: Shao
authors.forename: C Y
authors.initials: CY
authors.lastname: Tan
authors.forename: Y
authors.initials: Y
authors.lastname: Yao
mesh_terms: D015153:Blotting, Western; D002465:Cell Movement / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D004847:Epithelial Cells / Q000187:drug effects* / Q000502:physiology; D020868:Gene Silencing; D006801:Humans; D011228:Prealbumin / Q000378:metabolism / Q000494:pharmacology*; D012333:RNA, Messenger / Q000378:metabolism; D034741:RNA, Small Interfering; D055213:Retinal Pigment Epithelium / Q000187:drug effects* / Q000502:physiology; D012168:Retinal Pigments; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics / Q000378:metabolism
chemical_list: D011228:Prealbumin; D012333:RNA, Messenger; D034741:RNA, Small Interfering; D012168:Retinal Pigments; D042461:Vascular Endothelial Growth Factor A