title: Identification of Stages of Erythroid Differentiation in Bone Marrow and Erythrocyte Subpopulations in Blood Circulation that Are Preferentially Lost in Autoimmune Hemolytic Anemia in Mouse.
abstract: Repeated weekly injections of rat erythrocytes produced autoimmune hemolytic anemia (AIHA) in C57BL/6 mice after 5-6 weeks. Using the double in vivo biotinylation (DIB) technique, recently developed in our laboratory, turnover of erythrocyte cohorts of different age groups during AIHA was monitored. Results indicate a significant decline in the proportion of reticulocytes, young and intermediate age groups of erythrocytes, but a significant increase in the proportion of old erythrocytes in blood circulation. Binding of the autoantibody was relatively higher to the young erythrocytes and higher levels of intracellular reactive oxygen species (ROS) were also seen in these cells. Erythropoietic activity in the bone marrows and the spleen of AIHA induced mice was examined by monitoring the relative proportion of erythroid cells at various stages of differentiation in these organs. Cells at different stages of differentiation were enumerated flow cytometrically by double staining with anti-Ter119 and anti-transferrin receptor (CD71) monoclonal antibodies. Erythroid cells in bone marrow declined significantly in AIHA induced mice, erythroblast C being most affected (50% decline). Erythroblast C also recorded high intracellular ROS level along with increased levels of membrane-bound autoantibody. No such decline was observed in spleen. A model of AIHA has been proposed indicating that binding of autoantibodies may not be a sufficient condition for destruction of erythroid cells in bone marrow and in blood circulation. Last stage of erythropoietic differentiation in bone marrow and early stages of erythrocytes in blood circulation are specifically susceptible to removal in AIHA.
authors.affiliation: School of Life Sciences, Jawaharlal Nehru University, New Delhi, India.|Faculty of Life Sciences and Biotechnology, South Asian University, New Delhi, India.
authors.forename: Sreoshi
authors.initials: S
authors.lastname: Chatterjee
authors.affiliation: Faculty of Life Sciences and Biotechnology, South Asian University, New Delhi, India.
authors.forename: Nitin
authors.initials: N
authors.lastname: Bhardwaj
authors.affiliation: Faculty of Life Sciences and Biotechnology, South Asian University, New Delhi, India.
authors.forename: Rajiv K
authors.initials: RK
authors.lastname: Saxena
mesh_terms: D000744:Anemia, Hemolytic, Autoimmune / Q000097:blood* / Q000276:immunology; D000818:Animals; D001323:Autoantibodies / Q000378:metabolism*; D001854:Bone Marrow Cells / Q000166:cytology; D002454:Cell Differentiation; D004195:Disease Models, Animal; D004912:Erythrocytes / Q000166:cytology* / Q000276:immunology; D051379:Mice; D008810:Mice, Inbred C57BL; D051381:Rats; D017208:Rats, Wistar; D017382:Reactive Oxygen Species / Q000378:metabolism; D012156:Reticulocytes / Q000166:cytology* / Q000276:immunology; D013154:Spleen / Q000166:cytology
chemical_list: D001323:Autoantibodies; D017382:Reactive Oxygen Species