title: Infection by Rhodococcus fascians maintains cotyledons as a sink tissue for the pathogen.
abstract: Pisum sativum L. (pea) seed is a source of carbohydrate and protein for the developing plant. By studying pea seeds inoculated by the cytokinin-producing bacterium, Rhodococcus fascians , we sought to determine the impact of both an epiphytic (avirulent) strain and a pathogenic strain on source-sink activity within the cotyledons during and following germination.
  
  
  
  Bacterial spread was monitored microscopically, and real-time reverse transcription-quantitative PCR was used to determine the expression of cytokinin biosynthesis, degradation and response regulator gene family members, along with expression of family members of SWEET , SUT , CWINV and AAP genes - gene families identified initially in pea by transcriptomic analysis. The endogenous cytokinin content was also determined.
  
  
  
  The cotyledons infected by the virulent strain remained intact and turned green, while multiple shoots were formed and root growth was reduced. The epiphytic strain had no such marked impact. Isopentenyl adenine was elevated in the cotyledons infected by the virulent strain. Strong expression of RfIPT , RfLOG and RfCKX was detected in the cotyledons infected by the virulent strain throughout the experiment, with elevated expression also observed for PsSWEET , PsSUT and PsINV gene family members. The epiphytic strain had some impact on the expression of these genes, especially at the later stages of reserve mobilization from the cotyledons.
  
  
  
  The pathogenic strain retained the cotyledons as a sink tissue for the pathogen rather than the cotyledon converting completely to a source tissue for the germinating plant. We suggest that the interaction of cytokinins, CWINVs and SWEETs may lead to the loss of apical dominance and the appearance of multiple shoots.
authors.affiliation: School of Biological Sciences, University of Canterbury, Private Bag 4800, Christchurch 8140, New Zealand.
authors.forename: Pragatheswari
authors.initials: P
authors.lastname: Dhandapani
authors.affiliation: School of Biological Sciences, University of Canterbury, Private Bag 4800, Christchurch 8140, New Zealand.|School of Life Sciences, Yantai University, Yantai 264005, China.
authors.forename: Jiancheng
authors.initials: J
authors.lastname: Song
authors.affiliation: Laboratory of Growth Regulators, Centre of the Region Haná for Biotechnological and Agricultural Research, Institute of Experimental Botany CAS & Faculty of Science of Palacký University, Šlechtitelu 27, 783 71 Olomouc, Czech Republic.
authors.forename: Ondrej
authors.initials: O
authors.lastname: Novak
authors.affiliation: School of Biological Sciences, University of Canterbury, Private Bag 4800, Christchurch 8140, New Zealand.
authors.forename: Paula E
authors.initials: PE
authors.lastname: Jameson
mesh_terms: D018548:Cotyledon / Q000254:growth & development / Q000382:microbiology; D003583:Cytokinins / Q000378:metabolism; D018525:Germination; D054884:Host-Pathogen Interactions; D018532:Pisum sativum / Q000235:genetics* / Q000382:microbiology*; D010935:Plant Diseases / Q000382:microbiology*; D016133:Polymerase Chain Reaction; D012240:Rhodococcus / Q000502:physiology*; D012639:Seeds / Q000382:microbiology
chemical_list: D003583:Cytokinins
keywords: Apical dominance; Pisum sativum L.; Rhodococcus fascians; SWEET; amino acid transporter; cell wall invertase; cytokinin; cytokinin oxidase/dehydrogenase; pea; seed; sink and source; sucrose transporter