title: N-(3-oxo-hexanoyl)-homoserine lactone has a critical contribution to the quorum-sensing-dependent regulation in phytopathogen Pseudomonas syringae pv. tabaci 11528.
abstract: The phytopathogen Pseudomonas syringae pv. tabaci 11528 (P. syringae 11528), causing wild-fire disease in soybean and tobacco plants, processes PsyI-PsyR quorum-sensing (QS) system, in which PsyI is the N-(3-oxo-hexanoyl)-homoserine lactone (3OC6-HSL) synthase. In comparison to P. syringae 11528 AHL-deficient mutant, 845 3OC6-HSL-dependent genes were identified using RNA sequencing (RNA-seq) in the AHL-deficient mutant grown with exogenous 3OC6-HSL in the transition from the exponential to the stationary phase, and many of them were associated with virulence, which were negatively regulated. The gene ontology and KEGG pathway enrichment analysis of those genes presented that the most pronounced regulation was involved in bacterial motility. Moreover, similar expression profiles of genes during growth phases were observed in both the wild type and the AHL-deficient mutant with exogenous 3OC6-HSL compared with the AHL-deficient mutant. These findings imply that 3OC6-HSL has a critical contribution to the QS-dependent regulation on gene expression, and 3OC6-HSL-dependent regulation may play a significant role in plant infection.
authors.affiliation: Research Center for Eco-Environment Sciences, Chinese Academy of Sciences, Beijing 100085, China.|University of the Chinese Academy of Sciences, Beijing 100049, China.
authors.forename: Feifei
authors.initials: F
authors.lastname: Cheng
authors.affiliation: Research Center for Eco-Environment Sciences, Chinese Academy of Sciences, Beijing 100085, China gqzhuang@rcees.ac.cn azma@rcees.ac.cn.|University of the Chinese Academy of Sciences, Beijing 100049, China.
authors.forename: Anzhou
authors.initials: A
authors.lastname: Ma
authors.affiliation: Research Center for Eco-Environment Sciences, Chinese Academy of Sciences, Beijing 100085, China.|University of the Chinese Academy of Sciences, Beijing 100049, China.
authors.forename: Xuliang
authors.initials: X
authors.lastname: Zhuang
authors.affiliation: The Administrative Center for China's Agenda 21, Beijing 100038, China.
authors.forename: Xiaojia
authors.initials: X
authors.lastname: He
authors.affiliation: Research Center for Eco-Environment Sciences, Chinese Academy of Sciences, Beijing 100085, China gqzhuang@rcees.ac.cn azma@rcees.ac.cn.|University of the Chinese Academy of Sciences, Beijing 100049, China.
authors.forename: Guoqiang
authors.initials: G
authors.lastname: Zhuang
mesh_terms: D015964:Gene Expression Regulation, Bacterial / Q000235:genetics*; D006714:Homoserine / Q000031:analogs & derivatives* / Q000378:metabolism; D009068:Movement / Q000502:physiology; D010935:Plant Diseases / Q000382:microbiology; D044224:Pseudomonas syringae / Q000235:genetics / Q000472:pathogenicity*; D053038:Quorum Sensing / Q000235:genetics*; D013025:Glycine max / Q000382:microbiology; D014026:Nicotiana / Q000382:microbiology; D037521:Virulence Factors / Q000235:genetics*
chemical_list: D037521:Virulence Factors; C080518:N-(beta-oxooctan-1-oyl)homoserine lactone; D006714:Homoserine
keywords: motility; quorum sensing; Pseudomonas syringae pv. tabaci 11528; virulence; N-(3-oxo-hexanoyl)-homoserine lactone