title: Comparison of Chemical Compositions in Pseudostellariae Radix from Different Cultivated Fields and Germplasms by NMR-Based Metabolomics.
abstract: Pseudostellariae Radix (PR) is an important traditional Chinese medicine (TCM), which is consumed commonly for its positive health effects. However, the chemical differences of PR from different cultivated fields and germplasms are still unknown. In order to comprehensively compare the chemical compositions of PR from different cultivated fields, in this study, ¹H-NMR-based metabolomics coupled with high performance liquid chromatography (HPLC) were used to investigate the different metabolites in PR from five germplasms (jr, zs1, zs2, sb, and xc) cultivated in traditional fields (Jurong, Jiangsu, JSJR) and cultivated fields (Zherong, Fujian, FJZR). A total of 34 metabolites were identified based on ¹H-NMR data, and fourteen of them were found to be different in PR from JSJR and FJZR. The relative contents of alanine, lactate, lysine, taurine, sucrose, tyrosine, linolenic acid, γ-aminobutyrate, and hyperoside in PR from JSJR were higher than that in PR from FJZR, while PR from FJZR contained higher levels of glutamine, raffinose, xylose, unsaturated fatty acid, and formic acid. The contents of Heterophyllin A and Heterophyllin B were higher in PR from FJZR. This study will provide the basic information for exploring the influence law of ecological environment and germplasm genetic variation on metabolite biosynthesis of PR and its quality formation mechanism.
authors.affiliation: College of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, China. huayujiao1020@163.com.
authors.forename: Yujiao
authors.initials: Y
authors.lastname: Hua
authors.affiliation: College of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, China. yp0317@163.com.
authors.forename: Ya
authors.initials: Y
authors.lastname: Hou
authors.affiliation: College of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, China. jshmwsn@163.com.
authors.forename: Shengnan
authors.initials: S
authors.lastname: Wang
authors.affiliation: College of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, China. nzymayang1990@163.com.
authors.forename: Yang
authors.initials: Y
authors.lastname: Ma
authors.affiliation: College of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, China. liuzixiu3221@126.com.
authors.forename: Zixiu
authors.initials: Z
authors.lastname: Liu
authors.affiliation: College of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, China. ggn0117@sohu.com.
authors.forename: Lisi
authors.initials: L
authors.lastname: Zou
authors.affiliation: College of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, China. liuxunh1959@sohu.com.
authors.forename: Xunhong
authors.initials: X
authors.lastname: Liu
authors.affiliation: College of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, China. luoyiyuan0012@sohu.com.
authors.forename: Yiyuan
authors.initials: Y
authors.lastname: Luo
authors.affiliation: College of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, China. liujx0516@163.com.
authors.forename: Juanxiu
authors.initials: J
authors.lastname: Liu
mesh_terms: D004365:Drugs, Chinese Herbal / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D055442:Metabolome; D055432:Metabolomics / Q000379:methods; D018517:Plant Roots / Q000737:chemistry* / Q000378:metabolism; D066244:Proton Magnetic Resonance Spectroscopy; D064028:Tracheophyta / Q000737:chemistry* / Q000378:metabolism
chemical_list: D004365:Drugs, Chinese Herbal
keywords: 1H-NMR; Pseudostellariae Radix; cultivated fields; germplasms; metabolomics