title: MicroRNA-187 regulates gastric cancer progression by targeting the tumor suppressor CRMP1.
abstract: Aberrant expression of microRNAs contributes to the initiation and progression of numerous human cancers. The underlying effects and molecular mechanisms of microRNA-187 (miR-187) in gastric cancer (GC) remain unclear. The present study reports that miR-187 was significantly overexpressed in GC tissues compared to that in non-tumor tissues and was associated with malignant clinical factors such as depth of invasion (P = 0.005), tumor size (P = 0.024), lymph node metastasis (P = 0.048), and TNM stage (P = 0.035). Additionally, miR-187 promoted tumor growth in vivo, and significantly increased migration, invasion, and proliferation, but inhibited apoptosis in GC cells. It was found that collapsin response mediator protein 1 (CRMP1), a tumor suppressor, was a direct downstream target of miR-187 in GC. Furthermore, CRMP1 silencing resulted in similar effects on cell proliferation, migration, and apoptosis as those of miR-187 overexpressing GC cells. Additionally, the effects of miR-187 inhibitor on cell migration and cell apoptosis were reversed by CRMP1 downregulation. In summary, miR-187 promotes tumor progression by regulating CRMP1 expression in GC and may thus be a potential prognostic marker and a therapeutic target in GC.
authors.affiliation: Department of Gastrointestinal Surgery, Taihe Hospital, Hubei University of Medicine, Shiyan City, Hubei Province, China.
authors.forename: Lian
authors.initials: L
authors.lastname: Ren
authors.affiliation: Department of Ophthalmology, Renmin Hospital, Hubei University of Medicine, Shiyan City, Hubei Province, China.
authors.forename: Fang
authors.initials: F
authors.lastname: Li
authors.affiliation: Department of Gastrointestinal Surgery, Taihe Hospital, Hubei University of Medicine, Shiyan City, Hubei Province, China.
authors.forename: Maojun
authors.initials: M
authors.lastname: Di
authors.affiliation: Department of Gastrointestinal Surgery, Taihe Hospital, Hubei University of Medicine, Shiyan City, Hubei Province, China.
authors.forename: Yingfeng
authors.initials: Y
authors.lastname: Fu
authors.affiliation: Department of Gastrointestinal Surgery, Taihe Hospital, Hubei University of Medicine, Shiyan City, Hubei Province, China.
authors.forename: Yuanjian
authors.initials: Y
authors.lastname: Hui
authors.affiliation: Department of Gastrointestinal Surgery, Taihe Hospital, Hubei University of Medicine, Shiyan City, Hubei Province, China.
authors.forename: Gaochun
authors.initials: G
authors.lastname: Xiao
authors.affiliation: Department of Gastrointestinal Surgery, Taihe Hospital, Hubei University of Medicine, Shiyan City, Hubei Province, China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Sun
authors.affiliation: Department of Gastrointestinal Surgery, Taihe Hospital, Hubei University of Medicine, Shiyan City, Hubei Province, China.
authors.forename: Yanwei
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Department of Gastrointestinal Surgery, Taihe Hospital, Hubei University of Medicine, Shiyan City, Hubei Province, China.
authors.forename: Dan
authors.initials: D
authors.lastname: Ren
authors.affiliation: Department of Gastrointestinal Surgery, Taihe Hospital, Hubei University of Medicine, Shiyan City, Hubei Province, China. Electronic address: dx801426@163.com.
authors.forename: Xian
authors.initials: X
authors.lastname: Du
mesh_terms: D000818:Animals; D017209:Apoptosis; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D035683:MicroRNAs / Q000235:genetics*; D008875:Middle Aged; D009361:Neoplasm Invasiveness / Q000235:genetics / Q000473:pathology; D009419:Nerve Tissue Proteins / Q000235:genetics*; D010750:Phosphoproteins / Q000235:genetics*; D013270:Stomach / Q000473:pathology*; D013274:Stomach Neoplasms / Q000235:genetics* / Q000473:pathology*; D000098855:Collapsin Response Mediator Protein 1
chemical_list: C571843:MIRN187 microRNA, human; D035683:MicroRNAs; D009419:Nerve Tissue Proteins; D010750:Phosphoproteins; D000098855:Collapsin Response Mediator Protein 1
keywords: Apoptosis; CRMP1; Gastric cancer; Migration; Proliferation; miR-187