title: Optical Torque Wrench Design and Calibration.
abstract: Expanding the capabilities of optical traps with angular control of the trapped particle has numerous potential applications in all fields where standard linear optical tweezers are employed. Here we describe in detail the construction, alignment, and calibration of the Optical Torque Wrench, a mode of function that can be added to linear optical tweezers to simultaneously apply and measure both force and torque on birefringent microscopic cylindrical particles. The interaction between the linear polarization of the laser and the birefringent cylinder creates an angular trap for the particle orientation, described by a periodic potential. As a consequence of the experimental control of the tilt of the periodic potential, the dynamical excitability of the system can be observed. Angular optical tweezers remain less widespread than their linear counterpart. We hope this technical guide can foster their development and new applications.
authors.affiliation: Department of Single-Molecule Biophysics, Centre de Biochimie Structurale, CNRS UMR5048, UM, INSERM UMR1054, Montpellier, France.
authors.forename: Zhanna
authors.initials: Z
authors.lastname: Santybayeva
authors.affiliation: Department of Single-Molecule Biophysics, Centre de Biochimie Structurale, CNRS UMR5048, UM, INSERM UMR1054, Montpellier, France.
authors.forename: Francesco
authors.initials: F
authors.lastname: Pedaci
mesh_terms: D001718:Birefringence; D002138:Calibration; D008962:Models, Theoretical; D052898:Optical Tweezers; D055095:Optics and Photonics / Q000295:instrumentation / Q000379:methods
keywords: Birefringence; Optical tweezers; Polarization; Single-molecule biophysics; Torque