title: Effective degradation of aflatoxin B1using a novel thermophilic microbial consortium TADC7.
abstract: We constructed a novel thermophilic microbial consortium, TADC7, with stable and efficient aflatoxin B1(AFB1) degradation activity. The microbial consortium degraded more than 95% of the toxin within 72h when cultured with AFB1, and the optimum temperature was 55-60°C. TADC7 tolerated high doses of AFB1, with no inhibitory effects up to 5000μgL-1AFB1; moreover, the degradation kinetics fit well with the Monod model. The proteins or enzymes in the TADC7 cell-free supernatant played a major role in AFB1degradation. AFB1degradation by the cell-free supernatant was stable up to 90°C, with an optimal pH of 8-10. We performed 16S rRNA sequencing to determine TADC7 community structure dynamics; the results indicated that Geobacillus and Tepidimicrobium played major roles in AFB1degradation.
authors.affiliation: Institute of Agricultural Products Processing and Nuclear Agriculture Technology Research, Hubei Academy of Agricultural Sciences, Wuhan 430064, China; College of Biology and Pharmacy, Three Gorges University, Yichang 443002, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Institute of Agricultural Products Processing and Nuclear Agriculture Technology Research, Hubei Academy of Agricultural Sciences, Wuhan 430064, China; College of Biology and Pharmacy, Three Gorges University, Yichang 443002, China.
authors.forename: Chunxia
authors.initials: C
authors.lastname: Zhao
authors.affiliation: Institute of Marine Biology, Ocean College, Zhejiang University, Zhoushan 316021, Zhejiang, China.
authors.forename: Dongdong
authors.initials: D
authors.lastname: Zhang
authors.affiliation: Institute of Agricultural Products Processing and Nuclear Agriculture Technology Research, Hubei Academy of Agricultural Sciences, Wuhan 430064, China.
authors.forename: Mingming
authors.initials: M
authors.lastname: Zhao
authors.affiliation: Institute of Agricultural Products Processing and Nuclear Agriculture Technology Research, Hubei Academy of Agricultural Sciences, Wuhan 430064, China.
authors.forename: Dan
authors.initials: D
authors.lastname: Zheng
authors.affiliation: College of Biology and Pharmacy, Three Gorges University, Yichang 443002, China.
authors.forename: Yucai
authors.initials: Y
authors.lastname: Lyu
authors.affiliation: Institute of Agricultural Products Processing and Nuclear Agriculture Technology Research, Hubei Academy of Agricultural Sciences, Wuhan 430064, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Cheng
authors.affiliation: Institute of Agricultural Products Processing and Nuclear Agriculture Technology Research, Hubei Academy of Agricultural Sciences, Wuhan 430064, China. Electronic address: gp.pengguo@gmail.com.
authors.forename: Peng
authors.initials: P
authors.lastname: Guo
authors.affiliation: College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, China.
authors.forename: Zongjun
authors.initials: Z
authors.lastname: Cui
mesh_terms: D016604:Aflatoxin B1 / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D019149:Bioreactors / Q000382:microbiology*; D059013:Microbial Consortia; D012336:RNA, Ribosomal, 16S; D014874:Water Pollutants, Chemical / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D018508:Water Purification / Q000379:methods*
chemical_list: D012336:RNA, Ribosomal, 16S; D014874:Water Pollutants, Chemical; D016604:Aflatoxin B1
keywords: Aflatoxin B(1); Biodegradation; Microbial consortium; Thermophilic