title: Mast Cells Density Positive to Tryptase Correlate with Microvascular Density in both Primary Gastric Cancer Tissue and Loco-Regional Lymph Node Metastases from Patients That Have Undergone Radical Surgery.
abstract: Mast Cells (MCs) play a role in immune responses and more recently MCs have been involved in tumoral angiogenesis. In particular MCs can release tryptase, a potent in vivo and in vitro pro-angiogenic factor via proteinase-activated receptor-2 (PAR-2) activation and mitogen-activated protein kinase (MAPK) phosphorylation. MCs can release tryptase following c-Kit receptor activation. Nevertheless, no data are available concerning the relationship among MCs Density Positive to Tryptase (MCDPT) and Microvascular Density (MVD) in both primary gastric cancer tissue and loco-regional lymph node metastases. A series of 75 GC patients with stage T2-3N2-3M₀ (by AJCC for Gastric Cancer Seventh Edition) undergone to radical surgery were selected for the study. MCDPT and MVD were evaluated by immunohistochemistry and by image analysis system and results were correlated each to other in primary tumor tissue and in metastatic lymph nodes harvested. Furthermore, tissue parameters were correlated with important clinico-pathological features. A significant correlation between MCDPT and MVD was found in primary gastric cancer tissue and lymph node metastases. Pearsont-test analysis (rranged from 0.74 to 0.79;p-value ranged from 0.001 to 0.003). These preliminary data suggest that MCDPT play a role in angiogenesis in both primary tumor and in lymph node metastases from GC. We suggest that MCs and tryptase could be further evaluated as novel targets for anti-angiogenic therapies.
authors.affiliation: Department of Medical and Surgical Sciences, Clinical Surgery Unit, University "Magna Graecia" Medical School, Viale Europa, Germaneto, 88100 Catanzaro, Italy. michele.ammendola@libero.it.|Surgery Unit, National Cancer Research Centre, Istituto Tumori "Giovanni Paolo II", Viale Orazio Flacco 65, 70124 Bari, Italy. michele.ammendola@libero.it.
authors.forename: Michele
authors.initials: M
authors.lastname: Ammendola
authors.affiliation: Department of Medical and Surgical Sciences, Clinical Surgery Unit, University "Magna Graecia" Medical School, Viale Europa, Germaneto, 88100 Catanzaro, Italy. sacco@unicz.it.
authors.forename: Rosario
authors.initials: R
authors.lastname: Sacco
authors.affiliation: Pathology Unit, "Pugliese-Ciaccio" Hospital, Viale Pio X, 88100 Catanzaro, Italy. valezy@libero.it.
authors.forename: Valeria
authors.initials: V
authors.lastname: Zuccalà
authors.affiliation: Cardiovascular Disease Unit, "San Giovanni di Dio" Hospital, 88900 Crotone, Italy. marilyn_luposella@live.it.
authors.forename: Maria
authors.initials: M
authors.lastname: Luposella
authors.affiliation: Chair of Pathology, Veterinary Medical School, University "Aldo Moro" of Bari, Via Casamassima, 70010 Bari, Italy. rosavet@libero.it.
authors.forename: Rosa
authors.initials: R
authors.lastname: Patruno
authors.affiliation: Diagnostic and Interventional Radiology Unit with Integrated Section of Translational Medical Oncology, National Cancer Research Centre, Istituto Tumori "Giovanni Paolo II", viale Orazio Flacco 65, 70124 Bari, Italy. gadaleta5@libero.it.
authors.forename: Pietro
authors.initials: P
authors.lastname: Gadaleta
authors.affiliation: Chair of Pathology, Veterinary Medical School, University "Aldo Moro" of Bari, Via Casamassima, 70010 Bari, Italy. nicola.zizzo@uniba.it.
authors.forename: Nicola
authors.initials: N
authors.lastname: Zizzo
authors.affiliation: Diagnostic and Interventional Radiology Unit with Integrated Section of Translational Medical Oncology, National Cancer Research Centre, Istituto Tumori "Giovanni Paolo II", viale Orazio Flacco 65, 70124 Bari, Italy. c.gadaleta@oncologico.bari.it.
authors.forename: Cosmo Damiano
authors.initials: CD
authors.lastname: Gadaleta
authors.affiliation: Department of Health Science, Clinical Pharmacology and Pharmacovigilance Unit and Pharmacovigilance's Centre Calabria Region, University of Catanzaro "Magna Graecia" Medical School, Viale Europa, Germaneto, 88100 Catanzaro, Italy. desarro@unicz.it.
authors.forename: Giovambattista
authors.initials: G
authors.lastname: De Sarro
authors.affiliation: Department of Medical and Surgical Sciences, Clinical Surgery Unit, University "Magna Graecia" Medical School, Viale Europa, Germaneto, 88100 Catanzaro, Italy. sammarco@unicz.it.
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Sammarco
authors.affiliation: The Institute for Clinical Sciences, Department of Transplantation, University Hospital, Sahlgrenska Academy at the University of Gothenburg, 41345 Gothenburg, Sweden. mihai.oltean@surgery.gu.se.
authors.forename: Mihai
authors.initials: M
authors.lastname: Oltean
authors.affiliation: Diagnostic and Interventional Radiology Unit with Integrated Section of Translational Medical Oncology, National Cancer Research Centre, Istituto Tumori "Giovanni Paolo II", viale Orazio Flacco 65, 70124 Bari, Italy. iroran@tiscalinet.it.
authors.forename: Girolamo
authors.initials: G
authors.lastname: Ranieri
mesh_terms: D000368:Aged; D014408:Biomarkers, Tumor / Q000378:metabolism*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008198:Lymph Nodes / Q000201:enzymology / Q000473:pathology*; D008207:Lymphatic Metastasis; D008297:Male; D008407:Mast Cells / Q000201:enzymology / Q000473:pathology*; D008875:Middle Aged; D060787:Neoplasm Grading; D009367:Neoplasm Staging; D009389:Neovascularization, Pathologic / Q000175:diagnosis* / Q000201:enzymology / Q000473:pathology; D010865:Pilot Projects; D013274:Stomach Neoplasms / Q000175:diagnosis* / Q000201:enzymology / Q000473:pathology / Q000601:surgery; D053802:Tryptases / Q000378:metabolism*
chemical_list: D014408:Biomarkers, Tumor; D053802:Tryptases
keywords: angiogenesis; gastric cancer; mast cells; prognostic factor; therapy; tryptase