title: A Mixture of Extracts of Kochia scoparia and Rosa multiflora with PPAR α/γ Dual Agonistic Effects Prevents Photoaging in Hairless Mice.
abstract: Activation of peroxisome proliferator-activated receptors (PPAR) α/γ is known to inhibit the increases in matrix metalloproteinase (MMP) and reactive oxygen species (ROS) induced by ultraviolet light (UV). Extracts of natural herbs, such asKochia scopariaandRosa multiflora, have a PPAR α/γ dual agonistic effect. Therefore, we investigated whether and how they have an antiaging effect on photoaging skin. Eighteen-week-old hairless mice were irradiated with UVA 14 J/cm² and UVB 40 mJ/cm² three times a week for 8 weeks. A mixture of extracts ofKochia scopariaandRosa multiflora(KR) was topically applied on the dorsal skin of photoaging mice twice a day for 8 weeks. Tesaglitazar, a known PPAR α/γ agonist, and vehicle (propylene glycol:ethanol = 7:3,v/v) were applied as positive and negative controls, respectively. Dermal effects (including dermal thickness, collagen density, dermal expression of procollagen 1 and collagenase 13) and epidermal effects (including skin barrier function, epidermal proliferation, epidermal differentiation, and epidermal cytokines) were measured and compared. In photoaging murine skin, KR resulted in a significant recovery of dermal thickness as well as dermal fibroblasts, although it did not change dermal collagen density. KR increased the expression of dermal transforming growth factor (TGF)-β. The dermal effects of KR were explained by an increase in procollagen 1 expression, induced by TGF-β, and a decrease in MMP-13 expression. KR did not affect basal transepidermal water loss (TEWL) or stratum corneum (SC) integrity, but did decrease SC hydration. It also did not affect epidermal proliferation or epidermal differentiation. KR decreased the expression of epidermal interleukin (IL)-1α. Collectively, KR showed possible utility as a therapeutic agent for photoaging skin, with few epidermal side effects such as epidermal hyperplasia or poor differentiation.
authors.affiliation: Department of Dermatology, Yonsei University Wonju College of Medicine, Wonju 26426, Korea. moonlady84@naver.com.
authors.forename: Hyerin
authors.initials: H
authors.lastname: Jeon
authors.affiliation: Department of Dermatology, Yonsei University Wonju College of Medicine, Wonju 26426, Korea. donghye05@hanmail.net.
authors.forename: Dong Hye
authors.initials: DH
authors.lastname: Kim
authors.affiliation: Skin Research Team, Cosmax R&I Center, Seongnam 13486, Korea. yhno@cosmax.com.
authors.forename: Youn-Hwa
authors.initials: YH
authors.lastname: Nho
authors.affiliation: Skin Research Team, Cosmax R&I Center, Seongnam 13486, Korea. jepark@cosmax.com.
authors.forename: Ji-Eun
authors.initials: JE
authors.lastname: Park
authors.affiliation: Natural Skinomics Team, KIST Gangneung Institute of Natural Products, Gangneung 25451, Korea. snkim@kist.re.kr.
authors.forename: Su-Nam
authors.initials: SN
authors.lastname: Kim
authors.affiliation: Department of Dermatology, Yonsei University Wonju College of Medicine, Wonju 26426, Korea. choieh@yonsei.ac.kr.
authors.forename: Eung Ho
authors.initials: EH
authors.lastname: Choi
mesh_terms: D000818:Animals; D027467:Bassia scoparia / Q000737:chemistry*; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects / Q000528:radiation effects; D020781:Matrix Metalloproteinase 1 / Q000032:analysis / Q000235:genetics; D053509:Matrix Metalloproteinase 13 / Q000235:genetics; D008812:Mice, Hairless; D047493:PPAR alpha / Q000819:agonists* / Q000235:genetics; D047495:PPAR gamma / Q000819:agonists* / Q000235:genetics; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D011347:Procollagen / Q000235:genetics; D027826:Rosa / Q000737:chemistry*; D012867:Skin / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects / Q000648:ultrastructure; D015595:Skin Aging / Q000187:drug effects* / Q000528:radiation effects*; D016212:Transforming Growth Factor beta / Q000235:genetics; D014466:Ultraviolet Rays / Q000009:adverse effects
chemical_list: D047493:PPAR alpha; D047495:PPAR gamma; D010936:Plant Extracts; D011347:Procollagen; D016212:Transforming Growth Factor beta; D053509:Matrix Metalloproteinase 13; D020781:Matrix Metalloproteinase 1
keywords: PPAR α/γ; photoaging; procollagen 1; skin barrier; ultraviolet radiation