title: A Ramie bZIP Transcription Factor BnbZIP2 Is Involved in Drought, Salt, and Heavy Metal Stress Response.
abstract: bZIP transcription factors play key roles in plant growth, development, and stress signaling. A bZIP gene BnbZIP2 (GenBank accession number: KP642148) was cloned from ramie. BnbZIP2 has a 1416 base pair open reading frame, encoding a 471 amino acid protein containing a characteristic bZIP domain and a leucine zipper. BnbZIP2 shares high sequence similarity with bZIP factors from other plants. The BnbZIP2 protein is localized to both nuclei and cytoplasm. Transcripts of BnbZIP2 were found in various tissues in ramie, with significantly higher levels in female and male flowers. Its expression was induced by drought, high salinity, and abscisic acid treatments. Analysis of the cis-elements in promoters of BnbZIP2 identified cis-acting elements involved in growth, developmental processes, and a variety of stress responses. Transgenic Arabidopsis plants' overexpression of BnbZIP2 exhibited more sensitivity to drought and heavy metal Cd stress during seed germination, whereas more tolerance to high-salinity stress than the wild type during both seed germination and plant development. Thus, BnbZIP2 may act as a positive regulator in plants' response to high-salinity stress and be an important candidate gene for molecular breeding of salt-tolerant plants.
authors.affiliation: 1 Institute of Ramie, Hunan Agricultural University , Changsha, China .|2 Dazhou Institute of Agricultural Sciences , Dazhou, China .
authors.forename: Chengjian
authors.initials: C
authors.lastname: Huang
authors.affiliation: 1 Institute of Ramie, Hunan Agricultural University , Changsha, China .|3 College of Agronomy, Hunan Agricultural University , Changsha, China .
authors.forename: Jinghua
authors.initials: J
authors.lastname: Zhou
authors.affiliation: 1 Institute of Ramie, Hunan Agricultural University , Changsha, China .|3 College of Agronomy, Hunan Agricultural University , Changsha, China .|4 Hunan Provincial Crop Germplasm Innovation and Utilization Laboratory , Changsha, China .
authors.forename: Yucheng
authors.initials: Y
authors.lastname: Jie
authors.affiliation: 1 Institute of Ramie, Hunan Agricultural University , Changsha, China .|3 College of Agronomy, Hunan Agricultural University , Changsha, China .|4 Hunan Provincial Crop Germplasm Innovation and Utilization Laboratory , Changsha, China .
authors.forename: Hucheng
authors.initials: H
authors.lastname: Xing
authors.affiliation: 4 Hunan Provincial Crop Germplasm Innovation and Utilization Laboratory , Changsha, China .|5 College of Bioscience and Biotechnology, Hunan Agricultural University , Changsha, China .
authors.forename: Yingli
authors.initials: Y
authors.lastname: Zhong
authors.affiliation: 1 Institute of Ramie, Hunan Agricultural University , Changsha, China .|4 Hunan Provincial Crop Germplasm Innovation and Utilization Laboratory , Changsha, China .
authors.forename: Weilin
authors.initials: W
authors.lastname: Yu
authors.affiliation: 1 Institute of Ramie, Hunan Agricultural University , Changsha, China .|3 College of Agronomy, Hunan Agricultural University , Changsha, China .
authors.forename: Wei
authors.initials: W
authors.lastname: She
authors.affiliation: 1 Institute of Ramie, Hunan Agricultural University , Changsha, China .|3 College of Agronomy, Hunan Agricultural University , Changsha, China .|4 Hunan Provincial Crop Germplasm Innovation and Utilization Laboratory , Changsha, China .
authors.forename: Yushen
authors.initials: Y
authors.lastname: Ma
authors.affiliation: 1 Institute of Ramie, Hunan Agricultural University , Changsha, China .|3 College of Agronomy, Hunan Agricultural University , Changsha, China .
authors.forename: Zehang
authors.initials: Z
authors.lastname: Liu
authors.affiliation: 1 Institute of Ramie, Hunan Agricultural University , Changsha, China .|3 College of Agronomy, Hunan Agricultural University , Changsha, China .|4 Hunan Provincial Crop Germplasm Innovation and Utilization Laboratory , Changsha, China .
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D000040:Abscisic Acid / Q000494:pharmacology; D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D001483:Base Sequence; D050976:Basic-Leucine Zipper Transcription Factors / Q000235:genetics* / Q000378:metabolism; D032402:Boehmeria / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D055864:Droughts; D035264:Flowers / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D008353:Mannitol / Q000494:pharmacology; D019697:Onions / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D018515:Plant Leaves / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D018517:Plant Roots / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D030821:Plants, Genetically Modified; D011401:Promoter Regions, Genetic; D054712:Salinity; D055049:Salt Tolerance / Q000235:genetics*; D036226:Seedlings / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment; D012965:Sodium Chloride / Q000494:pharmacology; D013312:Stress, Physiological / Q000235:genetics*
chemical_list: D050976:Basic-Leucine Zipper Transcription Factors; D010940:Plant Proteins; D008353:Mannitol; D012965:Sodium Chloride; D000040:Abscisic Acid
keywords: Boehmeria nivea; abiotic stress; bZIP; promoter; transgenic plant