title: Association of the LOX-1 rs1050283 Polymorphism with Risk for Atherosclerotic Cerebral Infarction and its Effect on sLOX-1 and LOX-1 Expression in a Chinese Population.
abstract: OBJECTIVE
  The interaction between lectin-like oxidized low density lipoprotein (LDL) receptor-1 (LOX-1) and oxidized LDL (ox-LDL) has been viewed as an important pathogenic factor for cardiovascular diseases. This study aimed to explore the association of a functional polymorphism rs1050283 in the 3'-untranslated region of the LOX-1 gene with atherosclerotic cerebral infarction (ACI) susceptibility, and we also investigated the effects of the rs1050283 polymorphism on LOX-1 expression and serum levels of sLOX-1 in patients with ACI.
  
  
  METHODS
  A case-controlled study was performed in 526 patients with ACI and 640 healthy controls. Genotyping was performed by DNA sequencing method. Real-time PCR and Western blotting were used to determine the level of LOX-1 expression. Serum levels of sLOX-1 were quantified using ELISA according to the manufacturer's instruction.
  
  
  RESULTS
  The results of the present study showed that the frequency of rs1050283 T allele was significantly higher in patients with ACI than in healthy controls. We also found that the rs1050283 polymorphism T allele was associated with increased LOX-1 expression at mRNA and protein levels in patients with ACI. Furthermore, we also observed that among patients with ACI, those with the rs1050283 T allele showed an increased serum level of sLOX-1.
  
  
  CONCLUSIONS
  Our research demonstrated that the rs1050283 T allele of LOX-1 is strongly associated with an increased risk for ACI in a Chinese population, which also affects levels of LOX-1 and sLOX-1.
authors.affiliation: Department of Pharmacy, The Third Xiangya Hospital, Central South University.
authors.forename: Xin
authors.initials: X
authors.lastname: Guo
authors.affiliation: Department of Pharmacy, The Third Xiangya Hospital, Central South University.
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Xiang
authors.affiliation: Department of Neurology, The Third Xiangya Hospital, Central South University.
authors.forename: Heng
authors.initials: H
authors.lastname: Yang
authors.affiliation: Department of Pharmacy, The Third Xiangya Hospital, Central South University.
authors.forename: Lijin
authors.initials: L
authors.lastname: Yu
authors.affiliation: Department of Pharmacy, The Third Xiangya Hospital, Central South University.
authors.forename: Xiangdong
authors.initials: X
authors.lastname: Peng
authors.affiliation: Department of Pharmacy, The Third Xiangya Hospital, Central South University.
authors.forename: Ren
authors.initials: R
authors.lastname: Guo
mesh_terms: D000368:Aged; D000483:Alleles; D050197:Atherosclerosis / Q000208:ethnology / Q000235:genetics*; D016022:Case-Control Studies; D002544:Cerebral Infarction / Q000208:ethnology / Q000235:genetics*; D002681:China; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005786:Gene Expression Regulation; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D037102:Lectins / Q000737:chemistry; D008077:Lipoproteins, LDL / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D060888:Real-Time Polymerase Chain Reaction; D051127:Scavenger Receptors, Class E / Q000097:blood* / Q000235:genetics*
chemical_list: D037102:Lectins; D008077:Lipoproteins, LDL; C495137:OLR1 protein, human; D051127:Scavenger Receptors, Class E; C099252:oxidized low density lipoprotein
keywords: Atherosclerotic cerebral infarction; Lectin-like oxidized LDL receptor-1; Oxidized low density lipoprotein; rs1050283 polymorphism