title: Diagnostic application of oxidation-reduction potential assay for measurement of oxidative stress: clinical utility in male factor infertility.
abstract: The objectives of this study were to: (i) describe a protocol measuring the oxidation-reduction potential (ORP) by MiOXSYS System as an alternative method of seminal oxidative stress (OS) testing; (ii) establish a reference value for static ORP (sORP) to distinguish between controls and male factor infertility patients; (iii) evaluate intra-observer and inter-observer reliability; and (iv) examine association of sORP with sperm parameters predictive of male factor infertility. Elevated levels of sORP were seen in infertile patients (6.22 ± 1.10 mV/106sperm/ml) compared with controls (1.59 ± 0.29 mV/106sperm/ml) (P = 0.004). A sORP cut-off value 1.36 mV/106sperm/ml identified normal semen and abnormal semen quality with a sensitivity 69.6%, specificity 83.1%, positive predictive value 85.3% and negative predictive value 65.9%. The test demonstrated strong intra-observer (CV 8.39%) and inter-observer reliability (correlations >0.97). Higher sORP levels were associated with poor sperm parameters across the fertility status of subjects. Negative correlations were noted with sperm parameters (concentration, total sperm count, motility and morphology) indicating these male infertility parameters are related to OS. In conclusion, the introduction of ORP as a novel clinical test for assessment of OS will help clinicians to better diagnose and manage male factor infertility patients.
authors.affiliation: American Center for Reproductive Medicine, Cleveland Clinic, Cleveland, OH 44195, USA. Electronic address: agarwaa@ccf.org.
authors.forename: Ashok
authors.initials: A
authors.lastname: Agarwal
authors.affiliation: American Center for Reproductive Medicine, Cleveland Clinic, Cleveland, OH 44195, USA; Department of Life Science and Bioinformatics, Assam University, Silchar 788011, India.
authors.forename: Shubhadeep
authors.initials: S
authors.lastname: Roychoudhury
authors.affiliation: American Center for Reproductive Medicine, Cleveland Clinic, Cleveland, OH 44195, USA.
authors.forename: Rakesh
authors.initials: R
authors.lastname: Sharma
authors.affiliation: American Center for Reproductive Medicine, Cleveland Clinic, Cleveland, OH 44195, USA.
authors.forename: Sajal
authors.initials: S
authors.lastname: Gupta
authors.affiliation: Department of Urology, Cleveland Clinic, Cleveland, OH 44195, USA.
authors.forename: Ahmad
authors.initials: A
authors.lastname: Majzoub
authors.affiliation: Department of Urology, Cleveland Clinic, Cleveland, OH 44195, USA.
authors.forename: Edmund
authors.initials: E
authors.lastname: Sabanegh
mesh_terms: D019540:Area Under Curve; D016022:Case-Control Studies; D005260:Female; D005298:Fertility; D006801:Humans; D007248:Infertility, Male / Q000175:diagnosis*; D008297:Male; D015588:Observer Variation; D010084:Oxidation-Reduction; D018384:Oxidative Stress; D011237:Predictive Value of Tests; D011446:Prospective Studies; D015203:Reproducibility of Results; D012661:Semen; D055101:Semen Analysis; D012680:Sensitivity and Specificity; D013076:Sperm Count; D013081:Sperm Motility; D013094:Spermatozoa / Q000502:physiology
keywords: clinical test; male infertility; oxidation-reduction potential; oxidative stress; reference value; semen