title: Single-Layer Ternary Chalcogenide Nanosheet as a Fluorescence-Based "Capture-Release" Biomolecular Nanosensor.
abstract: The novel application of two-dimensional (2D) single-layer ternary chalcogenide nanosheets as "capture-release" fluorescence-based biomolecular nanosensors is demonstrated. Fluorescently labeled biomolecular probe is first captured by the ultrathin Ta2NiS5nanosheets and then released upon adding analyte containing a target biomolecule due to the higher probe-target affinity. Here, the authors use a nucleic acid probe for the model target biomolecule Plasmodium lactate dehydrogenase, which is an important malarial biomarker. The ultrathin Ta2NiS5nanosheet serves as a highly efficient fluorescence quencher and the nanosensor developed from the nanosheet is highly sensitive and specific toward the target biomolecule. Apart from the specificity toward the target biomolecule in homogeneous solutions, the developed nanosensor is capable of detecting and differentiating the target in heterogeneous solutions consisting of either a mixture of biomolecules or serum, with exceptional specificity. The simplicity of the "capture-release" method, by eliminating the need for preincubation of the probe with the test sample, may facilitate further development of portable and rapid biosensors. The authors anticipate that this ternary chalcogenide nanosheet-based biomolecular nanosensor will be useful for the rapid detection and differentiation of a wide range of chemical and biological species.
authors.affiliation: NUS Graduate School for Integrative Sciences and Engineering, National University of Singapore, Singapore, 117456, Singapore.|Centre for Advanced 2D Materials and Graphene Research Centre, National University of Singapore, Singapore, 117543, Singapore.|Department of Biomedical Engineering, National University of Singapore, Singapore, 117576, Singapore.
authors.lastname: Kenry
authors.affiliation: Department of Biomedical Engineering, National University of Singapore, Singapore, 117576, Singapore.
authors.forename: Alisha
authors.initials: A
authors.lastname: Geldert
authors.affiliation: School of Materials Science and Engineering, Nanyang Technological University, Singapore, 639798, Singapore.
authors.forename: Zhuangchai
authors.initials: Z
authors.lastname: Lai
authors.affiliation: School of Materials Science and Engineering, Nanyang Technological University, Singapore, 639798, Singapore.
authors.forename: Ying
authors.initials: Y
authors.lastname: Huang
authors.affiliation: School of Materials Science and Engineering, Nanyang Technological University, Singapore, 639798, Singapore.
authors.forename: Peng
authors.initials: P
authors.lastname: Yu
authors.affiliation: School of Materials Science and Engineering, Nanyang Technological University, Singapore, 639798, Singapore.
authors.forename: Chaoliang
authors.initials: C
authors.lastname: Tan
authors.affiliation: School of Materials Science and Engineering, Nanyang Technological University, Singapore, 639798, Singapore.
authors.forename: Zheng
authors.initials: Z
authors.lastname: Liu
authors.affiliation: School of Materials Science and Engineering, Nanyang Technological University, Singapore, 639798, Singapore.
authors.forename: Hua
authors.initials: H
authors.lastname: Zhang
authors.affiliation: NUS Graduate School for Integrative Sciences and Engineering, National University of Singapore, Singapore, 117456, Singapore.|Centre for Advanced 2D Materials and Graphene Research Centre, National University of Singapore, Singapore, 117543, Singapore.|Department of Biomedical Engineering, National University of Singapore, Singapore, 117576, Singapore.|Mechanobiology Institute, National University of Singapore, Singapore, 117411, Singapore.
authors.forename: Chwee Teck
authors.initials: CT
authors.lastname: Lim
mesh_terms: D015374:Biosensing Techniques; D005453:Fluorescence; D018625:Microscopy, Atomic Force; D049329:Nanostructures / Q000737:chemistry*
keywords: 2D nanomaterials; biomolecules; fluorescence quenching; nanosensors; transition metal chalcogenides