title: Molecular assessment of the transplacental transmission of Toxoplasma gondii, Neospora caninum, Brucella canis and Ehrlichia canis in dogs.
abstract: Given the fact that numerous microbial species can be detected in pregnant female dogs, the objective of this study was to assess the transplacental transmission of Brucella canis, Ehrlichia canis, Neospora caninum and Toxoplasma gondii in stillborn puppies. This study involved 41 stillborn puppies, 78.6% of which were positive for T. gondii, 52.4% for N. caninum and 59.5% for B. canis. E. canis was not detected in any of the analyzed puppies. Pregnancy is an important physiological condition for the transmission of infectious agents to puppies and transplacental transmission may be epidemiologically relevant in the spread of these opportunistic agents.
authors.affiliation: Laboratory of Virology and Rickettsial Infections, Veterinary Hospital of the Faculty of Veterinary Medicine, Federal University of Mato Grosso, Av. Fernando Correa da Costa 2367, Cuiabá 78090-900, MT, Brazil.
authors.forename: Isis Indaiara Gonçalves Granjeiro
authors.initials: IIGG
authors.lastname: Taques
authors.affiliation: Laboratory of Virology and Rickettsial Infections, Veterinary Hospital of the Faculty of Veterinary Medicine, Federal University of Mato Grosso, Av. Fernando Correa da Costa 2367, Cuiabá 78090-900, MT, Brazil.
authors.forename: Tatiane Rodrigues
authors.initials: TR
authors.lastname: Barbosa
authors.affiliation: Small Animals Surgery Clinic, Veterinary Hospital, of the Faculty of Veterinary Medicine, Federal University of Mato Grosso, Av. Fernando Correa da Costa 2367, Cuiabá 78090-900, MT, Brazil.
authors.forename: Andresa de Cássia
authors.initials: AC
authors.lastname: Martini
authors.affiliation: Laboratory of Veterinary Microbiology, Veterinary Hospital, of the Faculty of Veterinary Medicine, Federal University of Mato Grosso, Av. Fernando Correa da Costa 2367, Cuiabá 78090-900, MT, Brazil.
authors.forename: Letícia Camara
authors.initials: LC
authors.lastname: Pitchenin
authors.affiliation: Laboratory of Virology and Rickettsial Infections, Veterinary Hospital of the Faculty of Veterinary Medicine, Federal University of Mato Grosso, Av. Fernando Correa da Costa 2367, Cuiabá 78090-900, MT, Brazil.
authors.forename: Ísis Assis
authors.initials: ÍA
authors.lastname: Braga
authors.affiliation: Laboratory of Virology and Rickettsial Infections, Veterinary Hospital of the Faculty of Veterinary Medicine, Federal University of Mato Grosso, Av. Fernando Correa da Costa 2367, Cuiabá 78090-900, MT, Brazil.
authors.forename: Andréia Lima Tomé
authors.initials: ALT
authors.lastname: de Melo
authors.affiliation: Laboratory of Molecular Biology, Veterinary Hospital, of the Faculty of Veterinary Medicine, Federal University of Mato Grosso, Av. Fernando Correa da Costa 2367, Cuiabá 78090-900, MT, Brazil.
authors.forename: Luciano
authors.initials: L
authors.lastname: Nakazato
authors.affiliation: Laboratory of Veterinary Microbiology, Veterinary Hospital, of the Faculty of Veterinary Medicine, Federal University of Mato Grosso, Av. Fernando Correa da Costa 2367, Cuiabá 78090-900, MT, Brazil.
authors.forename: Valéria
authors.initials: V
authors.lastname: Dutra
authors.affiliation: Laboratory of Virology and Rickettsial Infections, Veterinary Hospital of the Faculty of Veterinary Medicine, Federal University of Mato Grosso, Av. Fernando Correa da Costa 2367, Cuiabá 78090-900, MT, Brazil. Electronic address: danmoura@ufmt.br.
authors.forename: Daniel Moura
authors.initials: DM
authors.lastname: de Aguiar
mesh_terms: D000818:Animals; D041002:Brucella canis / Q000235:genetics / Q000302:isolation & purification; D002006:Brucellosis / Q000382:microbiology / Q000635:transmission*; D003048:Coccidiosis / Q000382:microbiology / Q000635:transmission*; D004283:Dog Diseases / Q000382:microbiology / Q000469:parasitology / Q000635:transmission*; D004285:Dogs; D041061:Ehrlichia canis / Q000235:genetics / Q000302:isolation & purification; D016873:Ehrlichiosis / Q000382:microbiology / Q000635:transmission*; D005260:Female; D018445:Infectious Disease Transmission, Vertical / Q000662:veterinary*; D018688:Neospora / Q000235:genetics / Q000302:isolation & purification; D010920:Placenta / Q000382:microbiology / Q000469:parasitology; D016133:Polymerase Chain Reaction; D011247:Pregnancy; D011251:Pregnancy Complications, Infectious / Q000382:microbiology; D015597:Pregnancy Complications, Parasitic / Q000469:parasitology; D050497:Stillbirth / Q000662:veterinary*; D014122:Toxoplasma / Q000235:genetics / Q000302:isolation & purification; D014124:Toxoplasmosis, Animal / Q000469:parasitology / Q000635:transmission*
keywords: Bacteria; Pregnant bitch; Protozoa; Stillbirth