title: In vivo synthesis of europium selenide nanoparticles and related cytotoxicity evaluation of human cells.
abstract: Nanotechnology strives to combine new materials for development of noble nanoparticles. As the nanoparticles exhibit unique optical, electronic, and magnetic properties depending on their composition, developing safe, cost-effective and environmentally friendly technologies for the synthesis have become an important issue. In this study, in vivo synthesis of europium selenide (EuSe) nanoparticles was performed using recombinant Escherichia coli cells expressing heavy-metal binding proteins, phytochelatin synthase and metallothionein. The formation of EuSe nanoparticles was confirmed by using UV-vis spectroscopy, spectrofluorometry, X-ray diffraction, energy dispersive X-ray and transmission electron microscopy. The synthesized EuSe nanoparticles exhibited high fluorescence intensities as well as strong magnetic properties. Furthermore, anti-cancer effect of EuSe nanoparticles against cancer cell lines was investigated. This strategy for the biogenic synthesis of nanoparticles has a great potential as bioimaging tools and drug carrying agents in biomedical fields due to its simplicity and nontoxicity.
authors.affiliation: Department of Chemistry, Chung-Ang University, 84 Heukseok-ro, Dongjak-gu, Seoul 06974, South Korea.
authors.forename: Eun Bee
authors.initials: EB
authors.lastname: Kim
authors.affiliation: Department of Chemistry, Chung-Ang University, 84 Heukseok-ro, Dongjak-gu, Seoul 06974, South Korea.
authors.forename: Ji Min
authors.initials: JM
authors.lastname: Seo
authors.affiliation: Department of Chemistry, Chung-Ang University, 84 Heukseok-ro, Dongjak-gu, Seoul 06974, South Korea.
authors.forename: Gi Wook
authors.initials: GW
authors.lastname: Kim
authors.affiliation: Department of Chemical & Biomolecular Engineering, Department of Bio & Brain Engineering, Department of Biological Sciences, BioProcess Engineering Research Center, Center for Systems & Synthetic Biotechnology, Institute for the BioCentury, and Bioinformatics Research Center, KAIST, 291 Daehak-ro, Yuseong-gu, Daejeon 305-701, South Korea.
authors.forename: Sang Yup
authors.initials: SY
authors.lastname: Lee
authors.affiliation: Department of Chemistry, Chung-Ang University, 84 Heukseok-ro, Dongjak-gu, Seoul 06974, South Korea. Electronic address: tjpark@cau.ac.kr.
authors.forename: Tae Jung
authors.initials: TJ
authors.lastname: Park
mesh_terms: D019881:Aminoacyltransferases / Q000235:genetics / Q000378:metabolism; D000970:Antineoplastic Agents / Q000737:chemistry / Q000494:pharmacology; D001709:Biotechnology; D045744:Cell Line, Tumor; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D005063:Europium / Q000737:chemistry* / Q000494:pharmacology* / Q000633:toxicity; D055772:Green Chemistry Technology; D057809:HEK293 Cells; D006367:HeLa Cells; D006801:Humans; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000633:toxicity / Q000648:ultrastructure; D008668:Metallothionein / Q000235:genetics / Q000378:metabolism; D036103:Nanotechnology; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D018036:Selenium Compounds / Q000737:chemistry* / Q000494:pharmacology* / Q000633:toxicity
chemical_list: D000970:Antineoplastic Agents; D011994:Recombinant Proteins; D018036:Selenium Compounds; D005063:Europium; D008668:Metallothionein; D019881:Aminoacyltransferases; C093784:glutathione gamma-glutamylcysteinyltransferase
keywords: Biomedical application; EuSe nanoparticle; In vivo synthesis; Metal binding protein; Multi-functional properties