title: Selective reduction of IFN-γ single positive mycobacteria-specific CD4+ T cells in HIV-1 infected individuals with latent tuberculosis infection.
abstract: HIV-1 is recognized to increase the risk for tuberculosis even before CD4+ T cell deficiency is profound. To better understand how HIV-1 alters immunity to latent tuberculosis, we compared the magnitude and functional profile of mycobacteria-specific CD4+ T cells between HIV-uninfected and HIV-infected individuals, using flow cytometry. In HIV-1 infection, IFN-γ single positive mycobacteria-specific CD4+ T cells were decreased, while the frequency of polyfunctional cells (IFN-γ+IL-2+TNF-α+) remained unchanged. Moreover, the proportion of IFN-γ single positive cells correlated inversely with viral replication. Our results suggest that HIV-1 affects mycobacteria-specific cells differentially, depending on their functional capacity.
authors.affiliation: Division of Medical Virology, Department of Pathology, University of Cape Town, Cape Town, South Africa; Institute of Infectious Disease and Molecular Medicine, University of Cape Town, Cape Town, South Africa.
authors.forename: Catherine
authors.initials: C
authors.lastname: Riou
authors.affiliation: Division of Medical Virology, Department of Pathology, University of Cape Town, Cape Town, South Africa.
authors.forename: Rubina
authors.initials: R
authors.lastname: Bunjun
authors.affiliation: Division of Medical Virology, Department of Pathology, University of Cape Town, Cape Town, South Africa.
authors.forename: Tracey L
authors.initials: TL
authors.lastname: Müller
authors.affiliation: Division of Medical Virology, Department of Pathology, University of Cape Town, Cape Town, South Africa.
authors.forename: Agano
authors.initials: A
authors.lastname: Kiravu
authors.affiliation: Division of Medical Virology, Department of Pathology, University of Cape Town, Cape Town, South Africa.
authors.forename: Zekarias
authors.initials: Z
authors.lastname: Ginbot
authors.affiliation: Institute of Infectious Disease and Molecular Medicine, University of Cape Town, Cape Town, South Africa; Clinical Infectious Diseases Research Initiative, Institute of Infectious Disease and Molecular Medicine, University of Cape Town, Cape Town, South Africa; Division of Public Health Medicine, School of Public Health and Family Medicine, University of Cape Town, South Africa.
authors.forename: Tolu
authors.initials: T
authors.lastname: Oni
authors.affiliation: Institute of Infectious Disease and Molecular Medicine, University of Cape Town, Cape Town, South Africa; Clinical Infectious Diseases Research Initiative, Institute of Infectious Disease and Molecular Medicine, University of Cape Town, Cape Town, South Africa.
authors.forename: Rene
authors.initials: R
authors.lastname: Goliath
authors.affiliation: Institute of Infectious Disease and Molecular Medicine, University of Cape Town, Cape Town, South Africa; Clinical Infectious Diseases Research Initiative, Institute of Infectious Disease and Molecular Medicine, University of Cape Town, Cape Town, South Africa; The Francis Crick Institute Mill Hill Laboratory, London, United Kingdom; Department of Medicine, Imperial College London, United Kingdom.
authors.forename: Robert J
authors.initials: RJ
authors.lastname: Wilkinson
authors.affiliation: Division of Medical Virology, Department of Pathology, University of Cape Town, Cape Town, South Africa; Institute of Infectious Disease and Molecular Medicine, University of Cape Town, Cape Town, South Africa. Electronic address: wendy.burgers@uct.ac.za.
authors.forename: Wendy A
authors.initials: WA
authors.lastname: Burgers
mesh_terms: D017088:AIDS-Related Opportunistic Infections / Q000150:complications / Q000276:immunology* / Q000821:virology; D000328:Adult; D001800:Blood Specimen Collection / Q000379:methods; D018791:CD4 Lymphocyte Count; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology*; D005260:Female; D005434:Flow Cytometry; D015497:HIV-1 / Q000302:isolation & purification*; D006801:Humans; D007371:Interferon-gamma / Q000097:blood*; D055985:Latent Tuberculosis / Q000150:complications / Q000276:immunology*; D008297:Male; D009169:Mycobacterium tuberculosis / Q000276:immunology; D019562:Viral Load; D055815:Young Adult
chemical_list: D007371:Interferon-gamma
keywords: BCG; ESAT-6/CFP-10; HIV; LTBI; Memory; Mycobacterium tuberculosis; PPD; Polyfunction