title: Preparation and Physical Characterization of DNA-Binding Cationic Liposomes.
abstract: DNA-binding cationic liposomes are routinely employed as one of the major non-viral transfecting agents delivering DNA and other genes inside the cells. Cationic liposomes when complexed with DNA form a strong positively charged liposome-DNA complex or lipoplex. The chapter discusses primarily the major preparation technique for cationic liposomes and its physical characterization, with a focus on SYBR Green-I dye exclusion assay and DNA encapsulation enhancement by freeze-thaw technique. SYBR Green-I dye exclusion assay is a technique to determine the total amount of liposomal lipids required to bind a unit weight of DNA, which is critical for transfection experiments. Freeze-thaw technique on the other hand is one of the major techniques to improve DNA encapsulation in cationic liposomes.
authors.affiliation: Sancilio & Company, Inc., 2129 N. Congress Avenue, Riviera Beach, FL, 33404, USA. vaibhavsx@gmail.com.
authors.forename: Vaibhav
authors.initials: V
authors.lastname: Saxena
mesh_terms: D001671:Biochemistry / Q000379:methods*; D002412:Cations; D004247:DNA / Q000378:metabolism*; D005229:Fatty Acids, Monounsaturated / Q000737:chemistry; D008081:Liposomes / Q000138:chemical synthesis* / Q000737:chemistry*; D000644:Quaternary Ammonium Compounds / Q000737:chemistry
chemical_list: D002412:Cations; D005229:Fatty Acids, Monounsaturated; D008081:Liposomes; D000644:Quaternary Ammonium Compounds; D004247:DNA; C070046:1,2-dioleoyloxy-3-(trimethylammonium)propane
keywords: DNA; Dye exclusion; Encapsulation; Extrusion; Fluorescence; Freeze–thaw; Lipoplex; Liposomes; Transfection