title: Toxicity and oxidative stress induced by T-2 toxin in cultured mouse Leydig cells.
abstract: To explore the toxic mechanism of T-2 toxin on Leydig cells of mice, we would investigate the toxicity and oxidative stress induced by T-2 toxin in the cells. Leydig cells were isolated and cultured with control or T-2 toxin (10-7M, 10-8M, or 10-9M) for 24 h, then cells and supernatants were harvested to examine cell viability, superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and catalase (CAT) activities, expression of messenger RNA (mRNA) related to oxidative stress, malondialdehyde (MDA) content and DNA damage. The cell viability was evaluated in mouse Leydig cells by MTT assay, MDA content and SOD, GSH-Px and CAT activities were measured by routine kits, expression of mRNA related to oxidative stress were examined by quantitative real-time polymerase chain reaction (PCR), and DNA damage was investigated by comet assay. Leydig cells treated with T-2 toxin showed significant reductions in cell viability, SOD, GSH-Px and CAT activities, and expression of mRNA related to oxidative stress, and remarkable increases in MDA content and levels of DNA damage. This study proves that T-2 toxin is toxic to Leydig cells of mice. Furthermore, oxidative stress plays an important role in the above-mentioned negative effects of T-2 toxin.
authors.affiliation: a College of Medical Technology and Engineering , Henan University of Science and Technology , Luoyang , Henan , PR China.|b College of Food and Bioengineering , Henan University of Science and Technology , Luoyang , Henan , PR China.
authors.forename: Yong Fa
authors.initials: YF
authors.lastname: Zhang
authors.affiliation: a College of Medical Technology and Engineering , Henan University of Science and Technology , Luoyang , Henan , PR China.
authors.forename: Jian Ying
authors.initials: JY
authors.lastname: Yang
authors.affiliation: a College of Medical Technology and Engineering , Henan University of Science and Technology , Luoyang , Henan , PR China.
authors.forename: Yong Kai
authors.initials: YK
authors.lastname: Li
authors.affiliation: a College of Medical Technology and Engineering , Henan University of Science and Technology , Luoyang , Henan , PR China.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhou
mesh_terms: D000818:Animals; D002374:Catalase / Q000378:metabolism; D018929:Cell Culture Techniques; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D005979:Glutathione Peroxidase / Q000378:metabolism; D007985:Leydig Cells / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D051379:Mice; D018384:Oxidative Stress / Q000187:drug effects*; D013482:Superoxide Dismutase / Q000378:metabolism; D013605:T-2 Toxin / Q000633:toxicity*
chemical_list: D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D013605:T-2 Toxin
keywords: Leydig cells of mice; T-2 toxin; activities; mRNA expression; oxidative stress; toxicity