title: Comparison on extraction yield of sennoside A and sennoside B from senna (Cassia angustifolia) using conventional and non conventional extraction techniques and their quantification using a validated HPLC-PDA detection method.
abstract: Senna is an important medicinal plant and is used in many Ayurvedic formulations. Dianthraquinone glucosides are the main bioactive phytochemicals present in leaves and pods of senna. The extraction efficiency in terms of yield and composition of the extract of senna prepared using both conventional (cold percolation at room temperature and refluxing) and non conventional (ultrasound and microwave assisted solvent extraction as well as supercritical fluid extraction) techniques were compared in the present study. Also a rapid reverse phase HPLC-PDA detection method was developed and validated for the simultaneous determination of sennoside A and sennoside B in the different extracts of senna leaves. Ultrasound and microwave assisted solvent extraction techniques were more effective in terms of yield and composition of the extracts compared to cold percolation at room temperature and refluxing methods of extraction.
authors.affiliation: a Directorate of Medicinal and Aromatic Plants Research (Indian Council of Agricultural Research) , Anand , India.
authors.forename: Tushar
authors.initials: T
authors.lastname: Dhanani
authors.affiliation: a Directorate of Medicinal and Aromatic Plants Research (Indian Council of Agricultural Research) , Anand , India.
authors.forename: Raghuraj
authors.initials: R
authors.lastname: Singh
authors.affiliation: a Directorate of Medicinal and Aromatic Plants Research (Indian Council of Agricultural Research) , Anand , India.
authors.forename: Nagaraja
authors.initials: N
authors.lastname: Reddy
authors.affiliation: a Directorate of Medicinal and Aromatic Plants Research (Indian Council of Agricultural Research) , Anand , India.
authors.forename: A
authors.initials: A
authors.lastname: Trivedi
authors.affiliation: a Directorate of Medicinal and Aromatic Plants Research (Indian Council of Agricultural Research) , Anand , India.
authors.forename: Satyanshu
authors.initials: S
authors.lastname: Kumar
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D056148:Chromatography, Reverse-Phase; D025924:Chromatography, Supercritical Fluid; D018515:Plant Leaves / Q000737:chemistry; D012676:Senna Extract / Q000032:analysis / Q000302:isolation & purification*; D029846:Senna Plant / Q000737:chemistry*; D000081226:Sennosides
chemical_list: D000081226:Sennosides; D012676:Senna Extract
keywords: Cassia angustifolia; microwave assisted solvent extraction; sennosides; supercritical fluid extraction; ultrasound assisted solvent extraction