title: Protective effect of lycopene on fluoride-induced ameloblasts apoptosis and dental fluorosis through oxidative stress-mediated Caspase pathways.
abstract: Fluoride is an environmental toxicant and induces dental fluorosis and oxidative stress. Lycopene (LYC) is an effective antioxidant that is reported to attenuate fluoride toxicity. To determine the effects of LYC on sodium fluoride (NaF) -induced teeth and ameloblasts toxicity, rats were treated with NaF (10 mg/kg) and/or LYC (10 mg/kg) by orally administration for 5 weeks; ameloblasts were treated with NaF (5 mM) and/or LYC (2 μM) for 6 h. We found that the concentrations of fluoride, malondialdehyde (MDA) and reactive oxygen species (ROS), gene expressions and activities of Caspase-9 and Caspase-3, and the gene expressions of Bax were significantly decreased, while the activities of superoxide dismutase (SOD) and glutathione peroxidase (GPX), the gene expression of Bcl-2 were significantly increased in the LYC + NaF-treated rats group; concentrations of MDA and ROS, gene expressions and activities of Caspase-9 and Caspase-3, and the gene expression of Bax, and ameloblasts apoptosis rate were significantly decreased, while the activities of SOD and GPX, the gene expression of Bcl-2 were significantly increased in the LYC + NaF-treated ameloblasts group. These results suggest that LYC significantly combated NaF-induced ameloblasts apoptosis and dental fluorosis by attenuation oxidative stress and down-regulation Caspase pathway.
authors.affiliation: The Affiliated Stomatological Hospital of Jiamusi University, Jiamusi 154007, China. Electronic address: weishanli666@126.com.
authors.forename: Weishan
authors.initials: W
authors.lastname: Li
authors.affiliation: Hainan Stomatological Hospital, Haikou 570105, China.
authors.forename: Binghua
authors.initials: B
authors.lastname: Jiang
authors.affiliation: Hainan Stomatological Hospital, Haikou 570105, China.
authors.forename: Xianglin
authors.initials: X
authors.lastname: Cao
authors.affiliation: Daqing Oilfield General Hospital, Daqing 163000, China.
authors.forename: Yongjiang
authors.initials: Y
authors.lastname: Xie
authors.affiliation: The Affiliated Stomatological Hospital of Jiamusi University, Jiamusi 154007, China.
authors.forename: Ting
authors.initials: T
authors.lastname: Huang
mesh_terms: D000565:Ameloblasts / Q000187:drug effects / Q000201:enzymology / Q000473:pathology*; D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D002338:Carotenoids / Q000494:pharmacology*; D020169:Caspases / Q000235:genetics / Q000378:metabolism*; D015536:Down-Regulation / Q000187:drug effects; D009050:Fluorosis, Dental / Q000201:enzymology / Q000473:pathology*; D005979:Glutathione Peroxidase / Q000378:metabolism; D007180:Incisor / Q000187:drug effects; D000077276:Lycopene; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D051379:Mice; D018384:Oxidative Stress / Q000187:drug effects*; D020011:Protective Agents / Q000494:pharmacology*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D017207:Rats, Sprague-Dawley; D017382:Reactive Oxygen Species / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects; D012969:Sodium Fluoride / Q000633:toxicity*; D013482:Superoxide Dismutase / Q000378:metabolism; D051028:bcl-2-Associated X Protein / Q000235:genetics / Q000378:metabolism
chemical_list: D020011:Protective Agents; D012333:RNA, Messenger; D017382:Reactive Oxygen Species; D051028:bcl-2-Associated X Protein; D002338:Carotenoids; D008315:Malondialdehyde; D012969:Sodium Fluoride; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D020169:Caspases; D000077276:Lycopene
keywords: Fluoride; Lycopene; Oxidative stress; Rat