title: Fungal-Grade Reagents and Materials for Molecular Analysis.
abstract: Fungal DNA is present at very low loads in clinical specimens. Molecular detection by amplification assays generally is a challenge because of a potentially multiple input of contaminating DNA from exogenous sources. Besides airborne, handling and cross-contamination, materials and reagents used in the molecular laboratory can contain microbial DNA which is a long underestimated potential source of false positive results. In this contribution decontamination procedures of materials and reagents and the selection of certified microbial DNA-free components for sample collection, DNA extraction, and PCR amplification are discussed with respect to the aim of building up a reliable molecular system for the diagnosis of fungal organisms at the limit of detection.
authors.affiliation: Molzym GmbH & Co. KG, Mary-Astell-Str. 10, Bremen, 28359, Germany. lorenz@molzym.com.
authors.forename: Michael G
authors.initials: MG
authors.lastname: Lorenz
authors.affiliation: Molzym GmbH & Co. KG, Mary-Astell-Str. 10, Bremen, 28359, Germany.
authors.forename: Michael
authors.initials: M
authors.lastname: Lustig
authors.affiliation: Molzym GmbH & Co. KG, Mary-Astell-Str. 10, Bremen, 28359, Germany.
authors.forename: Marina
authors.initials: M
authors.lastname: Linow
mesh_terms: D059018:DNA Contamination; D004271:DNA, Fungal / Q000235:genetics / Q000302:isolation & purification*; D005658:Fungi / Q000235:genetics*; D006801:Humans; D025202:Molecular Diagnostic Techniques; D009181:Mycoses / Q000175:diagnosis* / Q000382:microbiology
chemical_list: D004271:DNA, Fungal
keywords: Contamination; DNA-free; Decontamination; Fungal DNA; Fungal DNA extraction; Fungal PCR reagents; Fungal assays