title: The Class II Trehalose 6-phosphate Synthase GenePvTPS9Modulates Trehalose Metabolism inPhaseolus vulgarisNodules.
abstract: Legumes form symbioses with rhizobia, producing nitrogen-fixing nodules on the roots of the plant host. The network of plant signaling pathways affecting carbon metabolism may determine the final number of nodules. The trehalose biosynthetic pathway regulates carbon metabolism and plays a fundamental role in plant growth and development, as well as in plant-microbe interactions. The expression of genes for trehalose synthesis during nodule development suggests that this metabolite may play a role in legume-rhizobia symbiosis. In this work,PvTPS9, which encodes a Class II trehalose-6-phosphate synthase (TPS) of common bean (Phaseolus vulgaris), was silenced by RNA interference in transgenic nodules. The silencing ofPvTPS9in root nodules resulted in a reduction of 85% (± 1%) of its transcript, which correlated with a 30% decrease in trehalose contents of transgenic nodules and in untransformed leaves. Composite transgenic plants withPvTPS9silenced in the roots showed no changes in nodule number and nitrogen fixation, but a severe reduction in plant biomass and altered transcript profiles of all Class IITPSgenes. Our data suggest thatPvTPS9plays a key role in modulating trehalose metabolism in the symbiotic nodule and, therefore, in the whole plant.
authors.affiliation: Departamento de Biología Molecular de Plantas, Instituto de Biotecnología/Universidad Nacional Autónoma de México Cuernavaca, Mexico.
authors.forename: Aarón
authors.initials: A
authors.lastname: Barraza
authors.affiliation: Departamento de Biología Molecular de Plantas, Instituto de Biotecnología/Universidad Nacional Autónoma de México Cuernavaca, Mexico.
authors.forename: Cecilia
authors.initials: C
authors.lastname: Contreras-Cubas
authors.affiliation: Departamento de Biología Molecular de Plantas, Instituto de Biotecnología/Universidad Nacional Autónoma de México Cuernavaca, Mexico.
authors.forename: Georgina
authors.initials: G
authors.lastname: Estrada-Navarrete
authors.affiliation: Departamento de Biología Molecular de Plantas, Instituto de Biotecnología/Universidad Nacional Autónoma de México Cuernavaca, Mexico.
authors.forename: José L
authors.initials: JL
authors.lastname: Reyes
authors.affiliation: Departamento de Biología Molecular de Plantas, Instituto de Biotecnología/Universidad Nacional Autónoma de México Cuernavaca, Mexico.
authors.forename: Marco A
authors.initials: MA
authors.lastname: Juárez-Verdayes
authors.affiliation: Centro de Investigación en Dinámica Celular, Universidad Autónoma del Estado de Morelos Cuernavaca, Mexico.
authors.forename: Nelson
authors.initials: N
authors.lastname: Avonce
authors.affiliation: Departamento de Biología Molecular de Plantas, Instituto de Biotecnología/Universidad Nacional Autónoma de México Cuernavaca, Mexico.
authors.forename: Carmen
authors.initials: C
authors.lastname: Quinto
authors.affiliation: Departamento de Biología Molecular de Plantas, Instituto de Biotecnología/Universidad Nacional Autónoma de México Cuernavaca, Mexico.
authors.forename: Claudia
authors.initials: C
authors.lastname: Díaz-Camino
authors.affiliation: Departamento de Biología Molecular de Plantas, Instituto de Biotecnología/Universidad Nacional Autónoma de México Cuernavaca, Mexico.
authors.forename: Federico
authors.initials: F
authors.lastname: Sanchez
keywords: AGO1-complex; Class II trehalose-6-phosphate synthase; Phaseolus vulgaris (common bean); legume-rhizobium symbiosis signaling; trehalose biosynthetic pathway