title: Investigation of the weak binding of a tetrahistidine-tagged peptide to quantum dots by using capillary electrophoresis with fluorescence detection.
abstract: Capillary electrophoresis with fluorescence detection was utilized to probe the self-assembly between cyanine group dye labeled tetrahistidine containing peptide and CdSe/ZnS quantum dots, inside the capillary. Quantum dots and cyanine group dye labeled tetrahistidine containing peptide were injected into the capillary one after the other and allowed to self-assemble. Their self-assembly resulted into a measurable Förster resonance energy transfer signal between quantum dots and cyanine group dye labeled tetrahistidine containing peptide. The Förster resonance energy transfer signal increased upon increasing the cyanine group dye labeled tetrahistidine containing peptide/quantum dot molar ratio and reached a plateau at the 32/1 molar ratio. Additionally, the Förster resonance energy transfer signal was also affected by the increment of the interval time of injection and the sampling time. Online ligand exchange experiments were used to assess, the potential of a monovalent ligand of imidazole and a hexavalent ligand peptide, to displace surface bound cyanine group dye labeled peptide ligands from the quantum dots surface. Under optimal conditions, a linear relationship between the integrated peak areas and hexavalent ligand peptide was obtained at a hexavalent ligand concentration range of 0-0.5 mM. Therefore, the present assay has the potential to be applied in the online ligands detection.
authors.affiliation: Institute of Pharmaceutical and Environmental Engineering, Changzhou Vocational Institute of Engineering, Changzhou, Jiangsu, P.R. China.
authors.forename: Haifang
authors.initials: H
authors.lastname: Qin
authors.affiliation: Kunshan affiliated Hospital of Nanjing University of Chinese Medicine, Kunshan, Jiangsu, P.R. China.
authors.forename: Xiyuan
authors.initials: X
authors.lastname: Jiang
authors.affiliation: School of Pharmaceutical Engineering and Life Science, Changzhou University, Changzhou, Jiangsu, P.R. China.
authors.forename: Jie
authors.initials: J
authors.lastname: Fan
authors.affiliation: School of Pharmaceutical Engineering and Life Science, Changzhou University, Changzhou, Jiangsu, P.R. China.
authors.forename: Jianpeng
authors.initials: J
authors.lastname: Wang
authors.affiliation: School of Pharmaceutical Engineering and Life Science, Changzhou University, Changzhou, Jiangsu, P.R. China.
authors.forename: Li
authors.initials: L
authors.lastname: Liu
authors.affiliation: School of Pharmaceutical Engineering and Life Science, Changzhou University, Changzhou, Jiangsu, P.R. China.
authors.forename: Lin
authors.initials: L
authors.lastname: Qiu
authors.affiliation: School of Pharmaceutical Engineering and Life Science, Changzhou University, Changzhou, Jiangsu, P.R. China.
authors.forename: Jianhao
authors.initials: J
authors.lastname: Wang
authors.affiliation: School of Pharmaceutical Engineering and Life Science, Changzhou University, Changzhou, Jiangsu, P.R. China.|Key Laboratory of Synthetic Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, P.R. China.
authors.forename: Pengju
authors.initials: P
authors.lastname: Jiang
mesh_terms: D002623:Chemistry Techniques, Analytical / Q000295:instrumentation*; D019075:Electrophoresis, Capillary; D031541:Fluorescence Resonance Energy Transfer; D008024:Ligands; D010455:Peptides / Q000378:metabolism*; D045663:Quantum Dots / Q000378:metabolism*
chemical_list: D008024:Ligands; D010455:Peptides
keywords: Förster resonance energy transfer; capillary electrophoresis; peptides; quantum dots; self-assembly