title: Purification and Characterization of a Thermostableβ-Mannanase fromBacillus subtilisBE-91: Potential Application in Inflammatory Diseases.
abstract: β-mannanase has shown compelling biological functions because of its regulatory roles in metabolism, inflammation, and oxidation. This study separated and purified theβ-mannanase fromBacillus subtilisBE-91, which is a powerful hemicellulose-degrading bacterium using a "two-step" method comprising ultrafiltration and gel chromatography. The purifiedβ-mannanase (about 28.2 kDa) showed high specific activity (79, 859.2 IU/mg). The optimum temperature and pH were 65°C and 6.0, respectively. Moreover, the enzyme was highly stable at temperatures up to 70°C and pH 4.5-7.0. Theβ-mannanase activity was significantly enhanced in the presence of Mn2+, Cu2+, Zn2+, Ca2+, Mg2+, and Al3+and strongly inhibited by Ba2+and Pb2+.KmandVmaxvalues for locust bean gum were 7.14 mg/mL and 107.5μmol/min/mL versus 1.749 mg/mL and 33.45µmol/min/mL for Konjac glucomannan, respectively. Therefore,β-mannanase purified by this work shows stability at high temperatures and in weakly acidic or neutral environments. Based on such data, theβ-mannanase will have potential applications as a dietary supplement in treatment of inflammatory processes.
authors.affiliation: Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, Changsha, Hunan 410205, China.
authors.forename: Lifeng
authors.identifier: 0000-0001-5820-7504
authors.initials: L
authors.lastname: Cheng
authors.affiliation: Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, Changsha, Hunan 410205, China.
authors.forename: Shengwen
authors.initials: S
authors.lastname: Duan
authors.affiliation: Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, Changsha, Hunan 410205, China.
authors.forename: Xiangyuan
authors.initials: X
authors.lastname: Feng
authors.affiliation: Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, Changsha, Hunan 410205, China.
authors.forename: Ke
authors.initials: K
authors.lastname: Zheng
authors.affiliation: Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, Changsha, Hunan 410205, China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Yang
authors.affiliation: Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, Changsha, Hunan 410205, China.
authors.forename: Zhengchu
authors.identifier: 0000-0001-7948-683X
authors.initials: Z
authors.lastname: Liu
mesh_terms: D001412:Bacillus subtilis / Q000737:chemistry*; D002850:Chromatography, Gel / Q000379:methods; D019587:Dietary Supplements; D006863:Hydrogen-Ion Concentration; D007249:Inflammation / Q000188:drug therapy*; D013696:Temperature; D014462:Ultrafiltration / Q000379:methods; D044902:beta-Mannosidase / Q000302:isolation & purification* / Q000494:pharmacology*
chemical_list: D044902:beta-Mannosidase