title: Isolation and chemoenzymatic treatment of glycoalkaloids from green, sprouting and rotting Solanum tuberosum potatoes for solanidine recovery.
abstract: The estimation of glycoalkaloids in the flesh of different types of decayed potatoes was evaluated. The results showed that turned green and also sprouting or rotting potato flesh contain high amounts of toxic solanine and chaconine, exceeding by 2-5-fold the recommended limit, and ranging from 2578±86mg/kg to 5063±230mg/kg of dry weight potato flesh. For safety consideration, these decayed potatoes should be systematically set aside. To avoid a net economic loss and encourage the removal of this hazardous food, a recycling process was investigated to generate added-value compounds from the toxic glycoalkaloids. A simple chemo-enzymatic protocol comprising a partial acidic hydrolysis followed by an enzymatic treatment with the β-glycosidase from Periplaneta americana allowed the efficient conversion of α-chaconine to solanidine.
authors.affiliation: Université de Toulouse; INSA, UPS, INP, LISBP, 135 Avenue de Rangueil, F-31077 Toulouse, France; CNRS, UMR 5504, F-31400 Toulouse, France; INRA, UMR 792 Ingénierie des Systèmes Biologiques et des Procédés, F-31400 Toulouse, France; Université Nangui Abrogoua Abidjan, Côte d'Ivoire.
authors.forename: Grokoré Yvonne
authors.initials: GY
authors.lastname: Koffi
authors.affiliation: Université de Toulouse; INSA, UPS, INP, LISBP, 135 Avenue de Rangueil, F-31077 Toulouse, France; CNRS, UMR 5504, F-31400 Toulouse, France; INRA, UMR 792 Ingénierie des Systèmes Biologiques et des Procédés, F-31400 Toulouse, France.
authors.forename: Magali
authors.initials: M
authors.lastname: Remaud-Simeon
authors.affiliation: Université Nangui Abrogoua Abidjan, Côte d'Ivoire.
authors.forename: Ahipo Edmond
authors.initials: AE
authors.lastname: Due
authors.affiliation: Université de Toulouse; INSA, UPS, INP, LISBP, 135 Avenue de Rangueil, F-31077 Toulouse, France; CNRS, UMR 5504, F-31400 Toulouse, France; INRA, UMR 792 Ingénierie des Systèmes Biologiques et des Procédés, F-31400 Toulouse, France. Electronic address: combes@insa-toulouse.fr.
authors.forename: Didier
authors.initials: D
authors.lastname: Combes
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid; D004144:Diosgenin / Q000032:analysis*; D006863:Hydrogen-Ion Concentration; D010526:Periplaneta / Q000201:enzymology; D012992:Solanine / Q000031:analogs & derivatives / Q000737:chemistry; D011198:Solanum tuberosum / Q000737:chemistry*; D001617:beta-Glucosidase / Q000378:metabolism
chemical_list: D012992:Solanine; C011860:alpha-chaconine; D001617:beta-Glucosidase; D004144:Diosgenin; C013255:solanidine
keywords: Potato glycoalkaloids; α-Chaconine; α-Solanine; β-Glycosidase; γ-Chaconine and solanidine recovery