title: Biodistribution of radiolabeled polyglutamic acid and PEG-polyglutamic acid nanocapsules.
abstract: Recently we reported the development of 100nm polyglutamic acid (PGA)-based nanocapsules, which were intended to carry anticancer drugs to the lymphatic system (Abellan-Pose et al., 2016). In this work, the objective was to further assess the potential "lympho-targeting" properties of radiolabeled111In-PGA and111In-PGA-PEG, following intravenous or subcutaneous administration. The results indicate that, following intravenous administration, both types of nanocapsules exhibit a modest accumulation in the lymph nodes (⩽2.3% ID/g). On the contrary, following subcutaneous administration, and irrespective of the presence of PEG on their surface, the nanocapsules were found to form a reservoir at the injection site, from which they drained slowly into the popliteal and the iliac lymph nodes. The significant accumulation of the radiolabeled nanocapsules in the lymph nodes was attained at 24 and 48h post-injection, reaching values comprised between 70% and 187% ID/g in the popliteal lymph nodes. Altogether, the results led us to validate our hypothesis about the ability of the PGA and PGA-PEG nanocapsules to reach the lymphatic system, especially following subcutaneous administration.
authors.affiliation: NanoBioFar Group, Center for Research in Molecular Medicine and Chronic Diseases, Health Research Institute of Santiago de Compostela (IDIS), University of Santiago de Compostela, 15706 Campus Vida, Santiago de Compostela, Spain.
authors.forename: Raquel
authors.initials: R
authors.lastname: Abellan-Pose
authors.affiliation: Dept. of Chemical Engineering and Pharmaceutical Technology, School of Pharmacy, Institute of Biomedical Technologies (ITB), Universidad de La Laguna, 38200 La Laguna, Spain.
authors.forename: Maria
authors.initials: M
authors.lastname: Rodríguez-Évora
authors.affiliation: NanoBioFar Group, Center for Research in Molecular Medicine and Chronic Diseases, Health Research Institute of Santiago de Compostela (IDIS), University of Santiago de Compostela, 15706 Campus Vida, Santiago de Compostela, Spain.
authors.forename: Sara
authors.initials: S
authors.lastname: Vicente
authors.affiliation: NanoBioFar Group, Center for Research in Molecular Medicine and Chronic Diseases, Health Research Institute of Santiago de Compostela (IDIS), University of Santiago de Compostela, 15706 Campus Vida, Santiago de Compostela, Spain.
authors.forename: Noemi
authors.initials: N
authors.lastname: Csaba
authors.affiliation: Dept. of Chemical Engineering and Pharmaceutical Technology, School of Pharmacy, Institute of Biomedical Technologies (ITB), Universidad de La Laguna, 38200 La Laguna, Spain.
authors.forename: Carmen
authors.initials: C
authors.lastname: Évora
authors.affiliation: NanoBioFar Group, Center for Research in Molecular Medicine and Chronic Diseases, Health Research Institute of Santiago de Compostela (IDIS), University of Santiago de Compostela, 15706 Campus Vida, Santiago de Compostela, Spain. Electronic address: mariaj.alonso@usc.es.
authors.forename: Maria Jose
authors.initials: MJ
authors.lastname: Alonso
authors.affiliation: Dept. of Chemical Engineering and Pharmaceutical Technology, School of Pharmacy, Institute of Biomedical Technologies (ITB), Universidad de La Laguna, 38200 La Laguna, Spain. Electronic address: adelgado@ull.es.
authors.forename: Araceli
authors.initials: A
authors.lastname: Delgado
mesh_terms: D000818:Animals; D005260:Female; D007205:Indium Radioisotopes / Q000737:chemistry*; D053769:Nanocapsules; D011092:Polyethylene Glycols / Q000737:chemistry*; D011099:Polyglutamic Acid / Q000493:pharmacokinetics*; D051381:Rats; D017207:Rats, Sprague-Dawley; D014018:Tissue Distribution
chemical_list: D007205:Indium Radioisotopes; D053769:Nanocapsules; D011099:Polyglutamic Acid; D011092:Polyethylene Glycols
keywords: Biodistribution; Lymphatic targeting; Nanocapsules; PEGylated polyglutamic acid; Polyglutamic acid