title: Human Dental Pulp Stem Cells Suppress Alloantigen-induced Immunity by Stimulating T Cells to Release Transforming Growth Factor Beta.
abstract: BACKGROUND
  Human dental pulp stem cells (hDPSCs) are ideal candidates for regenerating damaged dental tissue. To examine the possibility that hDPSCs may be used to regenerate pulp, we tested their in vitro effects on acute allogeneic immune responses.
  
  
  METHODS
  A peripheral blood mononuclear cell (PBMC) proliferation assay and immunoglobulin (Ig) production assay were performed to evaluate the immunosuppressive properties of hDPSCs.
  
  
  RESULTS
  The mixed lymphocyte reaction was suppressed by incubation with hDPSCs. Transforming growth factor beta (TGF-β) was the major soluble factor responsible for inhibiting the allogeneic proliferation of PBMCs. The production of IgM and IgG by allogeneic activation of responder B lymphocytes was also completely abrogated by TGF-β released from hDPSCs via interferon gamma in response to activation of the responder T lymphocytes.
  
  
  CONCLUSIONS
  hDPSCs inhibit acute allogeneic immune responses by their release of TGF-β as a result of allogeneic stimulation of T lymphocytes. This study provides an insight into the potential clinical use of hDPSCs for allogeneic transplantation.
authors.affiliation: Institute of Oral Biology, School of Dentistry, Graduate School, Kyung Hee University, Seoul, Republic of Korea.
authors.forename: Kyu Hwan
authors.initials: KH
authors.lastname: Kwack
authors.affiliation: Department of Conservative Dentistry, College of Dentistry, Kyung Hee University, Seoul, Republic of Korea.
authors.forename: Jung Min
authors.initials: JM
authors.lastname: Lee
authors.affiliation: Department of Conservative Dentistry, College of Dentistry, Kyung Hee University, Seoul, Republic of Korea. Electronic address: shpark94@khu.ac.kr.
authors.forename: Sang Hyuk
authors.initials: SH
authors.lastname: Park
authors.affiliation: Institute of Oral Biology, School of Dentistry, Graduate School, Kyung Hee University, Seoul, Republic of Korea. Electronic address: hyeonwoo@khu.ac.kr.
authors.forename: Hyeon Woo
authors.initials: HW
authors.lastname: Lee
mesh_terms: D000328:Adult; D003782:Dental Pulp / Q000166:cytology* / Q000276:immunology / Q000502:physiology; D006801:Humans; D007111:Immunity, Cellular / Q000276:immunology / Q000502:physiology; D007074:Immunoglobulin G / Q000378:metabolism; D007075:Immunoglobulin M / Q000378:metabolism; D007519:Isoantigens / Q000276:immunology*; D007963:Leukocytes, Mononuclear / Q000378:metabolism; D059630:Mesenchymal Stem Cells / Q000276:immunology* / Q000502:physiology; D013601:T-Lymphocytes / Q000378:metabolism / Q000502:physiology*; D016212:Transforming Growth Factor beta / Q000378:metabolism* / Q000502:physiology; D055815:Young Adult
chemical_list: D007074:Immunoglobulin G; D007075:Immunoglobulin M; D007519:Isoantigens; D016212:Transforming Growth Factor beta
keywords: Allogeneic transplantation; human dental pulp stem cells; immunosuppression; pulp regeneration; transforming growth factor beta