title: Axin2-expressing cells execute regeneration after skeletal injury.
abstract: The mammalian skeleton performs a diverse range of vital functions, requiring mechanisms of regeneration that restore functional skeletal cell populations after injury. We hypothesized that the Wnt pathway specifies distinct functional subsets of skeletal cell types, and that lineage tracing of Wnt-responding cells (WRCs) using the Axin2 gene in mice identifies a population of long-lived skeletal cells on the periosteum of long bone. Ablation of these WRCs disrupts healing after injury, and three-dimensional finite element modeling of the regenerate delineates their essential role in functional bone regeneration. These progenitor cells in the periosteum are activated upon injury and give rise to both cartilage and bone. Indeed, our findings suggest that WRCs may serve as a therapeutic target in the setting of impaired skeletal regeneration.
authors.affiliation: Hagey Laboratory for Pediatric Regenerative Medicine, Division of Plastic and Reconstructive Surgery, Department of Surgery, Stanford University School of Medicine, Stanford, CA 94305-5148, USA.|Institute for Stem Cell Biology and Regenerative Medicine, Stanford University School of Medicine, Stanford, CA 94305, USA.
authors.forename: R C
authors.initials: RC
authors.lastname: Ransom
authors.affiliation: Hagey Laboratory for Pediatric Regenerative Medicine, Division of Plastic and Reconstructive Surgery, Department of Surgery, Stanford University School of Medicine, Stanford, CA 94305-5148, USA.
authors.forename: D J
authors.initials: DJ
authors.lastname: Hunter
authors.affiliation: Hagey Laboratory for Pediatric Regenerative Medicine, Division of Plastic and Reconstructive Surgery, Department of Surgery, Stanford University School of Medicine, Stanford, CA 94305-5148, USA.
authors.forename: S
authors.initials: S
authors.lastname: Hyman
authors.affiliation: Hagey Laboratory for Pediatric Regenerative Medicine, Division of Plastic and Reconstructive Surgery, Department of Surgery, Stanford University School of Medicine, Stanford, CA 94305-5148, USA.
authors.forename: G
authors.initials: G
authors.lastname: Singh
authors.affiliation: Hagey Laboratory for Pediatric Regenerative Medicine, Division of Plastic and Reconstructive Surgery, Department of Surgery, Stanford University School of Medicine, Stanford, CA 94305-5148, USA.
authors.forename: S C
authors.initials: SC
authors.lastname: Ransom
authors.affiliation: Hagey Laboratory for Pediatric Regenerative Medicine, Division of Plastic and Reconstructive Surgery, Department of Surgery, Stanford University School of Medicine, Stanford, CA 94305-5148, USA.
authors.forename: E Z
authors.initials: EZ
authors.lastname: Shen
authors.affiliation: Hagey Laboratory for Pediatric Regenerative Medicine, Division of Plastic and Reconstructive Surgery, Department of Surgery, Stanford University School of Medicine, Stanford, CA 94305-5148, USA.
authors.forename: K C
authors.initials: KC
authors.lastname: Perez
authors.affiliation: Hagey Laboratory for Pediatric Regenerative Medicine, Division of Plastic and Reconstructive Surgery, Department of Surgery, Stanford University School of Medicine, Stanford, CA 94305-5148, USA.
authors.forename: M
authors.initials: M
authors.lastname: Gillette
authors.affiliation: Hagey Laboratory for Pediatric Regenerative Medicine, Division of Plastic and Reconstructive Surgery, Department of Surgery, Stanford University School of Medicine, Stanford, CA 94305-5148, USA.
authors.forename: J
authors.initials: J
authors.lastname: Li
authors.affiliation: Hagey Laboratory for Pediatric Regenerative Medicine, Division of Plastic and Reconstructive Surgery, Department of Surgery, Stanford University School of Medicine, Stanford, CA 94305-5148, USA.
authors.forename: B
authors.initials: B
authors.lastname: Liu
authors.affiliation: Hagey Laboratory for Pediatric Regenerative Medicine, Division of Plastic and Reconstructive Surgery, Department of Surgery, Stanford University School of Medicine, Stanford, CA 94305-5148, USA.
authors.forename: J B
authors.initials: JB
authors.lastname: Brunski
authors.affiliation: Hagey Laboratory for Pediatric Regenerative Medicine, Division of Plastic and Reconstructive Surgery, Department of Surgery, Stanford University School of Medicine, Stanford, CA 94305-5148, USA.
authors.forename: J A
authors.initials: JA
authors.lastname: Helms
mesh_terms: D000818:Animals; D060466:Axin Protein / Q000235:genetics / Q000378:metabolism*; D001842:Bone and Bones / Q000166:cytology* / Q000293:injuries*; D019070:Cell Lineage; D049109:Cell Proliferation; D020342:Finite Element Analysis; D008115:Liver Regeneration; D051379:Mice; D008962:Models, Theoretical; D060449:Wnt Signaling Pathway
chemical_list: D060466:Axin Protein; C111729:Axin2 protein, mouse