title: Assessment of organic micropollutants occurrence in treated wastewater using heat shock protein 47 stress responses in Chinese hamster ovary cells and GC/MS-based non-target screening.
abstract: Combining bioassays and analytical chemistry screening is a powerful approach to assess organic micropollutants which are the main contributors to toxic potential in complex mixtures of treated wastewater (TWW). The aim of this study was to perform a comprehensive toxicity assessment of treated effluents using stress response bioassays and then to assess the occurrence of the organic micropollutants which were responsible for this biological response using gas chromatography coupled with a mass spectrometry detector (GC/MS). Results showed that TWW samples induced significant stress response on Chinese hamster ovary cells, stably transfected with heat shock protein 47 promoter, at 0.1%, 1%, 5% and 10% concentrations. The organic chemical compounds responsible for stress response potential were identified at different percentage values using non-target chemical screening. Of the compounds detected in TWW1 and TWW4, 55.09% and 74.5% respectively, fell within the class of aliphatic hydrocarbons. Aliphatic hydrocarbons were also present in TWW3 at 26.46% whereas 11.96% corresponded to 6-acetyl-1,1,2,4,4,7-hexamethyltetralin and 16.08% to triethoxysilane. Moreover, 76.73% of TWW2 was recorded as decamethylcyclopentasiloxane (D5) and 17.44% as n-hexadecanoic acid.
authors.affiliation: Laboratory of Water Science and Technology (LSTE), National Agronomic Institute of Tunisia INAT, 43 Charles Nicolle Street Mahrajène, Tunis 1082, Tunisia.
authors.forename: Selma
authors.initials: S
authors.lastname: Etteieb
authors.affiliation: Graduate School of Life and Environmental Science, University of Tsukuba, 1-1-1 Tennodai, Tsukuba City, Ibaraki 305-8572, Japan and Alliance for Research on North Africa (ARENA), University of Tsukuba, 1-1-1 Tennodai, Tsukuba City, Ibaraki 305-8572, Japan E-mail: isoda.hiroko.ga@u.tsukuba.ac.jp.
authors.forename: Atsushi
authors.initials: A
authors.lastname: Kawachi
authors.affiliation: Graduate School of Life and Environmental Science, University of Tsukuba, 1-1-1 Tennodai, Tsukuba City, Ibaraki 305-8572, Japan and Alliance for Research on North Africa (ARENA), University of Tsukuba, 1-1-1 Tennodai, Tsukuba City, Ibaraki 305-8572, Japan E-mail: isoda.hiroko.ga@u.tsukuba.ac.jp.
authors.forename: Junkyu
authors.initials: J
authors.lastname: Han
authors.affiliation: International Center for Environmental Technologies of Tunis-CITET, Boulevard du Leader Yasser Arafat, Tunis 1080, Tunisia.
authors.forename: Foued
authors.initials: F
authors.lastname: Elayni
authors.affiliation: Laboratory of Water Science and Technology (LSTE), National Agronomic Institute of Tunisia INAT, 43 Charles Nicolle Street Mahrajène, Tunis 1082, Tunisia.
authors.forename: Jamila
authors.initials: J
authors.lastname: Tarhouni
authors.affiliation: Graduate School of Life and Environmental Science, University of Tsukuba, 1-1-1 Tennodai, Tsukuba City, Ibaraki 305-8572, Japan and Alliance for Research on North Africa (ARENA), University of Tsukuba, 1-1-1 Tennodai, Tsukuba City, Ibaraki 305-8572, Japan E-mail: isoda.hiroko.ga@u.tsukuba.ac.jp.
authors.forename: Hiroko
authors.initials: H
authors.lastname: Isoda
mesh_terms: D000818:Animals; D001681:Biological Assay; D016466:CHO Cells; D006224:Cricetinae; D003412:Cricetulus; D008401:Gas Chromatography-Mass Spectrometry; D050958:HSP47 Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism*; D009930:Organic Chemicals / Q000032:analysis / Q000633:toxicity*; D062065:Wastewater / Q000032:analysis / Q000633:toxicity; D014874:Water Pollutants, Chemical / Q000032:analysis / Q000633:toxicity*
chemical_list: D050958:HSP47 Heat-Shock Proteins; D009930:Organic Chemicals; D062065:Wastewater; D014874:Water Pollutants, Chemical