title: Photoactivation and inactivation of Arabidopsis cryptochrome 2.
abstract: Cryptochromes are blue-light receptors that regulate development and the circadian clock in plants and animals. We found that Arabidopsis cryptochrome 2 (CRY2) undergoes blue light-dependent homodimerization to become physiologically active. We identified BIC1 (blue-light inhibitor of cryptochromes 1) as an inhibitor of plant cryptochromes that binds to CRY2 to suppress the blue light-dependent dimerization, photobody formation, phosphorylation, degradation, and physiological activities of CRY2. We hypothesize that regulated dimerization governs homeostasis of the active cryptochromes in plants and other evolutionary lineages.
authors.affiliation: Basic Forestry and Proteomics Research Center, Fujian Agriculture and Forestry University, Fuzhou 350002, China.|Department of Molecular, Cell and Developmental Biology, University of California, Los Angeles, CA 90095, USA.
authors.forename: Qin
authors.initials: Q
authors.lastname: Wang
authors.affiliation: Basic Forestry and Proteomics Research Center, Fujian Agriculture and Forestry University, Fuzhou 350002, China.|College of Plant Science, Jilin University, Changchun 130062, China.
authors.forename: Zecheng
authors.initials: Z
authors.lastname: Zuo
authors.affiliation: Basic Forestry and Proteomics Research Center, Fujian Agriculture and Forestry University, Fuzhou 350002, China.|Department of Molecular, Cell and Developmental Biology, University of California, Los Angeles, CA 90095, USA.
authors.forename: Xu
authors.initials: X
authors.lastname: Wang
authors.affiliation: Basic Forestry and Proteomics Research Center, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
authors.forename: Lianfeng
authors.initials: L
authors.lastname: Gu
authors.affiliation: Biomass Engineering Research Division, RIKEN, Kanagawa 230-0045, Japan.
authors.forename: Takeshi
authors.initials: T
authors.lastname: Yoshizumi
authors.affiliation: Basic Forestry and Proteomics Research Center, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
authors.forename: Zhaohe
authors.initials: Z
authors.lastname: Yang
authors.affiliation: Basic Forestry and Proteomics Research Center, Fujian Agriculture and Forestry University, Fuzhou 350002, China.|College of Plant Science, Jilin University, Changchun 130062, China.
authors.forename: Liang
authors.initials: L
authors.lastname: Yang
authors.affiliation: Basic Forestry and Proteomics Research Center, Fujian Agriculture and Forestry University, Fuzhou 350002, China.|Department of Molecular, Cell and Developmental Biology, University of California, Los Angeles, CA 90095, USA.|College of Plant Science, Jilin University, Changchun 130062, China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Liu
authors.affiliation: College of Plant Science, Jilin University, Changchun 130062, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Liu
authors.affiliation: Department of Biotechnology and Kumho Life Science Laboratory, Chonnam National University, Gwangju 61186, Republic of Korea.
authors.forename: Yun-Jeong
authors.initials: YJ
authors.lastname: Han
authors.affiliation: Department of Biotechnology and Kumho Life Science Laboratory, Chonnam National University, Gwangju 61186, Republic of Korea.
authors.forename: Jeong-Il
authors.initials: JI
authors.lastname: Kim
authors.affiliation: Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 10081, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Liu
authors.affiliation: Department of Biological Chemistry, University of California, Los Angeles, CA 90095, USA.
authors.forename: James A
authors.initials: JA
authors.lastname: Wohlschlegel
authors.affiliation: Biomass Engineering Research Division, RIKEN, Kanagawa 230-0045, Japan.
authors.forename: Minami
authors.initials: M
authors.lastname: Matsui
authors.affiliation: Basic Forestry and Proteomics Research Center, Fujian Agriculture and Forestry University, Fuzhou 350002, China. yoshitooka@fafu.edu.cn clin@mcdb.ucla.edu.|Biomass Engineering Research Division, RIKEN, Kanagawa 230-0045, Japan.
authors.forename: Yoshito
authors.initials: Y
authors.lastname: Oka
authors.affiliation: Basic Forestry and Proteomics Research Center, Fujian Agriculture and Forestry University, Fuzhou 350002, China. yoshitooka@fafu.edu.cn clin@mcdb.ucla.edu.|Department of Molecular, Cell and Developmental Biology, University of California, Los Angeles, CA 90095, USA.
authors.forename: Chentao
authors.initials: C
authors.lastname: Lin
mesh_terms: D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism* / Q000528:radiation effects; D029681:Arabidopsis Proteins / Q000737:chemistry* / Q000235:genetics / Q000528:radiation effects; D056931:Cryptochromes / Q000737:chemistry* / Q000528:radiation effects*; D018506:Gene Expression Regulation, Plant / Q000528:radiation effects; D006706:Homeostasis; D008027:Light; D010766:Phosphorylation; D055668:Photochemical Processes; D030821:Plants, Genetically Modified / Q000235:genetics / Q000378:metabolism / Q000528:radiation effects; D011485:Protein Binding; D055503:Protein Multimerization / Q000528:radiation effects; D059748:Proteolysis / Q000528:radiation effects; D059467:Transcriptome / Q000528:radiation effects
chemical_list: D029681:Arabidopsis Proteins; C103618:CRY2 protein, Arabidopsis; D056931:Cryptochromes