title: The Mechanism Forming the Cell Surface of Tip-Growing Rooting Cells Is Conserved among Land Plants.
abstract: To discover mechanisms that controlled the growth of the rooting system in the earliest land plants, we identified genes that control the development of rhizoids in the liverwort Marchantia polymorpha. 336,000 T-DNA transformed lines were screened for mutants with defects in rhizoid growth, and a de novo genome assembly was generated to identify the mutant genes. We report the identification of 33 genes required for rhizoid growth, of which 6 had not previously been functionally characterized in green plants. We demonstrate that members of the same orthogroup are active in cell wall synthesis, cell wall integrity sensing, and vesicle trafficking during M. polymorpha rhizoid and Arabidopsis thaliana root hair growth. This indicates that the mechanism for constructing the cell surface of tip-growing rooting cells is conserved among land plants and was active in the earliest land plants that existed sometime more than 470 million years ago [1, 2].
authors.affiliation: Department of Plant Sciences, University of Oxford, South Parks Road, Oxford, OX1 3RB, UK.
authors.forename: Suvi
authors.initials: S
authors.lastname: Honkanen
authors.affiliation: Department of Plant Sciences, University of Oxford, South Parks Road, Oxford, OX1 3RB, UK.
authors.forename: Victor A S
authors.initials: VAS
authors.lastname: Jones
authors.affiliation: Department of Plant Sciences, University of Oxford, South Parks Road, Oxford, OX1 3RB, UK.
authors.forename: Giulia
authors.initials: G
authors.lastname: Morieri
authors.affiliation: Department of Plant Sciences, University of Oxford, South Parks Road, Oxford, OX1 3RB, UK.
authors.forename: Clement
authors.initials: C
authors.lastname: Champion
authors.affiliation: Department of Plant Sciences, University of Oxford, South Parks Road, Oxford, OX1 3RB, UK.
authors.forename: Alexander J
authors.initials: AJ
authors.lastname: Hetherington
authors.affiliation: Department of Plant Sciences, University of Oxford, South Parks Road, Oxford, OX1 3RB, UK.
authors.forename: Steve
authors.initials: S
authors.lastname: Kelly
authors.affiliation: Department of Plant Sciences, University of Oxford, South Parks Road, Oxford, OX1 3RB, UK.
authors.forename: Hélène
authors.initials: H
authors.lastname: Proust
authors.affiliation: Department of Plant Sciences, University of Oxford, South Parks Road, Oxford, OX1 3RB, UK.
authors.forename: Denis
authors.initials: D
authors.lastname: Saint-Marcoux
authors.affiliation: Department of Plant Sciences, University of Oxford, South Parks Road, Oxford, OX1 3RB, UK.
authors.forename: Helen
authors.initials: H
authors.lastname: Prescott
authors.affiliation: Department of Plant Sciences, University of Oxford, South Parks Road, Oxford, OX1 3RB, UK. Electronic address: liam.dolan@plants.ox.ac.uk.
authors.forename: Liam
authors.initials: L
authors.lastname: Dolan
mesh_terms: D017360:Arabidopsis / Q000235:genetics* / Q000254:growth & development*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D005075:Biological Evolution; D017124:Conserved Sequence; D018744:DNA, Plant / Q000235:genetics*; D018506:Gene Expression Regulation, Plant / Q000502:physiology; D047008:Marchantia; D010802:Phylogeny; D018517:Plant Roots / Q000166:cytology* / Q000254:growth & development*
chemical_list: D029681:Arabidopsis Proteins; D018744:DNA, Plant
keywords: Marchantia polymorpha; cell growth; rhizoid; root hair; tip growth