title: Target identification of volatile metabolites to allow the differentiation of lactic acid bacteria by gas chromatography-ion mobility spectrometry.
abstract: The purpose of this work was to study the potential of gas chromatography-ion mobility spectrometry (GC-IMS) to differentiate lactic acid bacteria (LAB) through target identification and fingerprints of volatile metabolites. The LAB selected were used as reference strains for their influence in the flavour of cheese. The four strains of LAB can be distinguished by the fingerprints generated by the volatile organic compounds (VOCs) emitted. 2-butanone, 2-pentanone, 2-heptanone and 3-methyl-1-butanol were identified as relevant VOCs for Lactobacillus casei and Lactobacillus paracasei subsp. paracasei. 2-Butanone and 3-methyl-1-butanol were identified in Lactococcus lactis subsp. lactis and Lactococcus cremoris subsp. cremoris. The IMS signals monitoring during a 24-30h period showed the growth of the LAB in vitro. The results demonstrated that GC-IMS is a useful technology for bacteria recognition and also for screening the aromatic potential of new isolates of LAB.
authors.affiliation: Food Science and Technology Department, University of Córdoba, Campus de Rabanales, 14071 Córdoba, Spain; Escuela Superior Politécnica de Chimborazo, Facultad de Ciencias. Riobamba, Ecuador.
authors.forename: Janneth
authors.initials: J
authors.lastname: Gallegos
authors.affiliation: Animal Production Department, University of Córdoba, Campus de Rabanales, 14071 Córdoba, Spain.
authors.forename: Cristina
authors.initials: C
authors.lastname: Arce
authors.affiliation: Food Science and Technology Department, University of Córdoba, Campus de Rabanales, 14071 Córdoba, Spain.
authors.forename: Rafael
authors.initials: R
authors.lastname: Jordano
authors.affiliation: Analytical Chemistry Department, University of Córdoba, Campus de Rabanales, 14071 Córdoba, Spain. Electronic address: lourdes.arce@uco.es.
authors.forename: Lourdes
authors.initials: L
authors.lastname: Arce
authors.affiliation: Food Science and Technology Department, University of Córdoba, Campus de Rabanales, 14071 Córdoba, Spain.
authors.forename: Luis M
authors.initials: LM
authors.lastname: Medina
mesh_terms: D002074:Butanones / Q000378:metabolism; D002611:Cheese / Q000382:microbiology*; D002849:Chromatography, Gas / Q000379:methods*; D005516:Food Microbiology; D007659:Ketones / Q000378:metabolism; D019344:Lactic Acid / Q000378:metabolism*; D007780:Lacticaseibacillus casei / Q000302:isolation & purification* / Q000378:metabolism; D013294:Lactococcus lactis / Q000302:isolation & purification* / Q000378:metabolism; D000439:Pentanols / Q000378:metabolism; D010422:Pentanones / Q000378:metabolism; D013057:Spectrum Analysis / Q000379:methods*
chemical_list: D002074:Butanones; D007659:Ketones; D000439:Pentanols; D010422:Pentanones; D019344:Lactic Acid; C005222:methylethyl ketone; C011917:2-heptanone; C029683:isopentyl alcohol; C076402:2-pentanone
keywords: IMS fingerprints; Lactic acid bacteria; Lactobacillus; Lactococcus; Metabolites; Volatile organic compounds