title: Crosstalk between sugarcane and a plant-growth promoting Burkholderia species.
abstract: Bacterial species in the plant-beneficial-environmental clade of Burkholderia represent a substantial component of rhizosphere microbes in many plant species. To better understand the molecular mechanisms of the interaction, we combined functional studies with high-resolution dual transcriptome analysis of sugarcane and root-associated diazotrophic Burkholderia strain Q208. We show that Burkholderia Q208 forms a biofilm at the root surface and suppresses the virulence factors that typically trigger immune response in plants. Up-regulation of bd-type cytochromes in Burkholderia Q208 suggests an increased energy production and creates the microaerobic conditions suitable for BNF. In this environment, a series of metabolic pathways are activated in Burkholderia Q208 implicated in oxalotrophy, microaerobic respiration, and formation of PHB granules, enabling energy production under microaerobic conditions. In the plant, genes involved in hypoxia survival are up-regulated and through increased ethylene production, larger aerenchyma is produced in roots which in turn facilitates diffusion of oxygen within the cortex. The detected changes in gene expression, physiology and morphology in the partnership are evidence of a sophisticated interplay between sugarcane and a plant-growth promoting Burkholderia species that advance our understanding of the mutually beneficial processes occurring in the rhizosphere.
authors.affiliation: School of Agriculture and Food Science, The University of Queensland, St Lucia, QLD 4072, Australia.|Institute for Molecular Bioscience, The University of Queensland, St Lucia, QLD 4072, Australia.
authors.forename: Chanyarat
authors.initials: C
authors.lastname: Paungfoo-Lonhienne
authors.affiliation: School of Chemistry and Molecular Biosciences, The University of Queensland, St Lucia, QLD 4072, Australia.
authors.forename: Thierry G A
authors.initials: TG
authors.lastname: Lonhienne
authors.affiliation: Institute for Molecular Bioscience, The University of Queensland, St Lucia, QLD 4072, Australia.|School of Chemistry and Molecular Biosciences, The University of Queensland, St Lucia, QLD 4072, Australia.|Australian Centre for Ecogenomics, The University of Queensland, St Lucia, QLD 4072, Australia.
authors.forename: Yun Kit
authors.initials: YK
authors.lastname: Yeoh
authors.affiliation: Advanced Water Management Centre and Centre for Microbial Electrosynthesis, The University of Queensland, St Lucia, QLD 4072, Australia.
authors.forename: Bogdan C
authors.initials: BC
authors.lastname: Donose
authors.affiliation: Centre for Microscopy and Microanalysis, The University of Queensland, St Lucia, QLD 4072, Australia.
authors.forename: Richard I
authors.initials: RI
authors.lastname: Webb
authors.affiliation: School of Chemistry and Molecular Biosciences, The University of Queensland, St Lucia, QLD 4072, Australia.|Australian Centre for Ecogenomics, The University of Queensland, St Lucia, QLD 4072, Australia.|QFAB Bioinformatics, The University of Queensland, St Lucia, QLD 4072, Australia.
authors.forename: Jeremy
authors.initials: J
authors.lastname: Parsons
authors.affiliation: Bioinformatics Resource EMBL Australia, The University of Queensland, St Lucia, QLD 4072, Australia.
authors.forename: Webber
authors.initials: W
authors.lastname: Liao
authors.affiliation: Sustainable Organic Solutions Pty Ltd, Indooroopilly, QLD 4068, Australia.
authors.forename: Evgeny
authors.initials: E
authors.lastname: Sagulenko
authors.affiliation: Sugar Research Australia, Indooroopilly, QLD 4068, Australia.
authors.forename: Prakash
authors.initials: P
authors.lastname: Lakshmanan
authors.affiliation: Institute for Molecular Bioscience, The University of Queensland, St Lucia, QLD 4072, Australia.|School of Chemistry and Molecular Biosciences, The University of Queensland, St Lucia, QLD 4072, Australia.|Australian Centre for Ecogenomics, The University of Queensland, St Lucia, QLD 4072, Australia.
authors.forename: Philip
authors.initials: P
authors.lastname: Hugenholtz
authors.affiliation: School of Agriculture and Food Science, The University of Queensland, St Lucia, QLD 4072, Australia.
authors.forename: Susanne
authors.initials: S
authors.lastname: Schmidt
authors.affiliation: Institute for Molecular Bioscience, The University of Queensland, St Lucia, QLD 4072, Australia.|Australian Research Council Centre of Excellence in Bioinformatics, The University of Queensland, St. Lucia, QLD 4072, Australia.
authors.forename: Mark A
authors.initials: MA
authors.lastname: Ragan
mesh_terms: D000693:Anaerobiosis; D018441:Biofilms / Q000254:growth & development; D019117:Burkholderia / Q000235:genetics / Q000502:physiology* / Q000648:ultrastructure; D002244:Carbon / Q000378:metabolism; D003580:Cytochromes / Q000378:metabolism; D015536:Down-Regulation / Q000235:genetics; D005407:Flagella / Q000378:metabolism; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D005798:Genes, Bacterial; D017343:Genes, Plant; D008070:Lipopolysaccharides / Q000096:biosynthesis; D053858:Metabolic Networks and Pathways / Q000235:genetics; D010788:Photosynthesis; D018517:Plant Roots / Q000382:microbiology / Q000648:ultrastructure; D031786:Saccharum / Q000254:growth & development* / Q000382:microbiology* / Q000648:ultrastructure; D017423:Sequence Analysis, RNA; D015854:Up-Regulation / Q000235:genetics
chemical_list: D003580:Cytochromes; D008070:Lipopolysaccharides; D002244:Carbon