title: Some factors affecting efficiency of the ultrasound-aided enzymatic hydrolysis of cotton cellulose.
abstract: The efficiency of the enzymatic hydrolysis of cellulose with low frequency ultrasound (horn-type reactor) was investigated and characterized by the concentration of reducing sugars liberated. Small squares of bleached cotton fabric were used for comparing the efficiency of different agitation methods (i.e. magnetic stirring, horizontal and vertical mechanical agitation) and ultrasound. At the same enzyme dosage and substrate level, sonication at 40, 60 and 80% amplitudes (Idiss: 16.2, 32.2 and 43.4W/cm2, respectively) intensified the hydrolysis over the most efficient mechanical agitation (i.e. magnetic stirring) alone by 15%, 24% and 54%, respectively. For mapping the ultrasonicated field, fabric layers positioned perpendicularly to the ultrasonic probe at different distances were hydrolysed. The optimal operating conditions were reached at 60% amplitude and 9mm The yield depended mainly on important factors such as amplitude, the presence of a reflector, distance from horn and form of substrate.
authors.affiliation: Department of Physical Chemistry and Materials Science, Faculty of Chemical Technology and Biotechnology, Budapest University of Technology and Economics, H-1521 Budapest, P.O. Box 91, Hungary.
authors.forename: Orsolya Erzsebet
authors.initials: OE
authors.lastname: Szabo
authors.affiliation: Department of Physical Chemistry and Materials Science, Faculty of Chemical Technology and Biotechnology, Budapest University of Technology and Economics, H-1521 Budapest, P.O. Box 91, Hungary. Electronic address: ecsiszar@mail.bme.hu.
authors.forename: Emilia
authors.initials: E
authors.lastname: Csiszar
mesh_terms: D002482:Cellulose / Q000378:metabolism*; D003368:Gossypium / Q000737:chemistry; D006868:Hydrolysis; D013010:Sonication; D013784:Textiles
chemical_list: D002482:Cellulose
keywords: Conventional agitation; Cotton cellulose; Enzymatic hydrolysis; Mapping of ultrasonic field; Ultrasound