title: Fine mapping of the chromosome 5B region carrying closely linked rust resistance genes Yr47 and Lr52 in wheat.
abstract: Fine mapping of Yr47 and Lr52 in chromosome arm 5BS of wheat identified close linkage of the marker sun180 to both genes and its robustness for marker-assisted selection was demonstrated. The widely effective and genetically linked rust resistance genes Yr47 and Lr52 have previously been mapped in the short arm of chromosome 5B in two F3populations (Aus28183/Aus27229 and Aus28187/Aus27229). The Aus28183/Aus27229 F3population was advanced to generate an F6recombinant inbred line (RIL) population to identify markers closely linked with Yr47 and Lr52. Diverse genomic resources including flow-sorted chromosome survey sequence contigs representing the orthologous region in Brachypodium distachyon, the physical map of chromosome arm 5BS, expressed sequence tags (ESTs) located in the 5BS6-0.81-1.00 deletion bin and resistance gene analog contigs of chromosome arm 5BS were used to develop markers to saturate the target region. Selective genotyping was also performed using the iSelect 90 K Infinium wheat SNP assay. A set of SSR, STS, gene-based and SNP markers were developed and genotyped on the Aus28183/Aus27229 RIL population. Yr47 and Lr52 are genetically distinct genes that mapped 0.4 cM apart in the RIL population. The SSR marker sun180 co-segregated with Lr52 and mapped 0.4 cM distal to Yr47. In a high resolution mapping population of 600 F2genotypes Yr47 and Lr52 mapped 0.2 cM apart and marker sun180 was placed 0.4 cM distal to Lr52. The amplification of a different sun180 amplicon (195 bp) than that linked with Yr47 and Lr52 (200 bp) in 204 diverse wheat genotypes demonstrated its robustness for marker-assisted selection of these genes.
authors.affiliation: Faculty of Agriculture, Food and Natural Resources, The University of Sydney Plant Breeding Institute, Private Bag 4011, Narellan, NSW, 2567, Australia.
authors.forename: Naeela
authors.initials: N
authors.lastname: Qureshi
authors.affiliation: Faculty of Agriculture, Food and Natural Resources, The University of Sydney Plant Breeding Institute, Private Bag 4011, Narellan, NSW, 2567, Australia.
authors.forename: Harbans
authors.initials: H
authors.lastname: Bariana
authors.affiliation: Department of Economic Development, Jobs, Transport and Resources, La Trobe University AgriBio, Bundoora, VIC, 3083, Australia.
authors.forename: Kerrie
authors.initials: K
authors.lastname: Forrest
authors.affiliation: Department of Economic Development, Jobs, Transport and Resources, La Trobe University AgriBio, Bundoora, VIC, 3083, Australia.
authors.forename: Matthew
authors.initials: M
authors.lastname: Hayden
authors.affiliation: Department of Plant and Microbial Biology, University of Zurich, Zollikerstrasse 107, 8008, Zurich, Switzerland.
authors.forename: Beat
authors.initials: B
authors.lastname: Keller
authors.affiliation: Department of Plant and Microbial Biology, University of Zurich, Zollikerstrasse 107, 8008, Zurich, Switzerland.
authors.forename: Thomas
authors.initials: T
authors.lastname: Wicker
authors.affiliation: USDA-ARS Cereal Crops Research Unit, Red River Valley Agricultural Research Center, 1605 Albrecht BLVD, Fargo, ND, 58102-2765, USA.
authors.forename: Justin
authors.initials: J
authors.lastname: Faris
authors.affiliation: Institute of Cytology and Genetics SB RAS, Novosibirsk, Russia.
authors.forename: Elena
authors.initials: E
authors.lastname: Salina
authors.affiliation: Faculty of Agriculture, Food and Natural Resources, The University of Sydney Plant Breeding Institute, Private Bag 4011, Narellan, NSW, 2567, Australia. urmil.bansal@sydney.edu.au.
authors.forename: Urmil
authors.identifier: http://orcid.org/0000-0003-1119-4464
authors.initials: U
authors.lastname: Bansal
mesh_terms: D001487:Basidiomycota; D002874:Chromosome Mapping; D018744:DNA, Plant / Q000235:genetics; D060467:Disease Resistance / Q000235:genetics*; D020224:Expressed Sequence Tags; D017343:Genes, Plant; D008040:Genetic Linkage; D005819:Genetic Markers; D005838:Genotype; D018895:Microsatellite Repeats; D010641:Phenotype; D000069600:Plant Breeding; D010935:Plant Diseases / Q000235:genetics* / Q000382:microbiology; D020641:Polymorphism, Single Nucleotide; D016324:Sequence Tagged Sites; D014908:Triticum / Q000235:genetics* / Q000382:microbiology
chemical_list: D018744:DNA, Plant; D005819:Genetic Markers