title: Molecular detection and characterization ofBrucellaspecies in raw informally marketed milk from Uganda.
abstract: This study identified and characterizedBrucellaspecies in the informal milk chain in Uganda. A total of 324 cattle bulk milk samples were screened for the genusBrucellaby real-time PCR with primers targeting thebcsp31gene and further characterized by theomp25gene. Of the samples tested, 6.5% were positive forBrucellaspecies. In theomp25phylogeny, the study sequences were found to form a separate clade within the branch containingB. abortussequences. The study shows that informally marketed cattle milk in Uganda is a likely risk factor for human brucellosis and confirms thatB. abortusis present in the cattle population. This information is important for potential future control measures, such as vaccination of cattle.
authors.affiliation: Zoonosis Science Center, Department of Medical Biochemistry and Microbiology, Uppsala University, Uppsala, Sweden.|Department of Medical Science, Uppsala University, Uppsala, Sweden.
authors.forename: Tove
authors.initials: T
authors.lastname: Hoffman
authors.affiliation: Department of Biomedical Sciences and Veterinary Public Health, Swedish University of Agricultural Sciences, Uppsala, Sweden.
authors.forename: Kim
authors.initials: K
authors.lastname: Rock
authors.affiliation: College of Veterinary Medicine, Animal Resources and Bio-Security, Makerere University, Kampala, Uganda.
authors.forename: Denis Rwabiita
authors.initials: DR
authors.lastname: Mugizi
authors.affiliation: Zoonosis Science Center, Department of Medical Biochemistry and Microbiology, Uppsala University, Uppsala, Sweden.|Department of Biomedical Sciences and Veterinary Public Health, Swedish University of Agricultural Sciences, Uppsala, Sweden.|Division of Clinical Microbiology, Department of Laboratory Medicine, Karolinska University Hospital, Stockholm, Sweden.
authors.forename: Shaman
authors.initials: S
authors.lastname: Muradrasoli
authors.affiliation: Department of Clinical Sciences, Swedish University of Agricultural Sciences, Uppsala, Sweden.
authors.forename: Elisabeth
authors.initials: E
authors.lastname: Lindahl-Rajala
authors.affiliation: College of Veterinary Medicine, Animal Resources and Bio-Security, Makerere University, Kampala, Uganda.
authors.forename: Joseph
authors.initials: J
authors.lastname: Erume
authors.affiliation: Department of Clinical Sciences, Swedish University of Agricultural Sciences, Uppsala, Sweden.
authors.forename: Ulf
authors.initials: U
authors.lastname: Magnusson
authors.affiliation: Zoonosis Science Center, Department of Medical Biochemistry and Microbiology, Uppsala University, Uppsala, Sweden.|Department of Medical Science, Uppsala University, Uppsala, Sweden.
authors.forename: Åke
authors.initials: Å
authors.lastname: Lundkvist
authors.affiliation: Department of Biomedical Sciences and Veterinary Public Health, Swedish University of Agricultural Sciences, Uppsala, Sweden; Sofia.Boqvist@slu.se.
authors.forename: Sofia
authors.initials: S
authors.lastname: Boqvist
keywords: Africa; PCR; bcsp31; brucellosis; bulk milk; milk delivery chain; omp25