title: High mannose-specific lectin Msl mediates key interactions of the vaginal Lactobacillus plantarum isolate CMPG5300.
abstract: To characterize the interaction potential of the human vaginal isolate Lactobacillus plantarum CMPG5300, its genome was mined for genes encoding lectin-like proteins. cmpg5300.05_29 was identified as the gene encoding a putative mannose-binding lectin. Phenotypic analysis of a gene knock-out mutant of cmpg5300.05_29 showed that expression of this gene is important for auto-aggregation, adhesion to the vaginal epithelial cells, biofilm formation and binding to mannosylated glycans. Purification of the predicted lectin domain of Cmpg5300.05_29 and characterization of its sugar binding capacity confirmed the specificity of the lectin for high- mannose glycans. Therefore, we renamed Cmpg5300.05_29 as a mannose-specific lectin (Msl). The purified lectin domain of Msl could efficiently bind to HIV-1 glycoprotein gp120 and Candida albicans, and showed an inhibitory activity against biofilm formation of uropathogenic Escherichia coli, Staphylococcus aureus and Salmonella Typhimurium. Thus, using a combination of molecular lectin characterization and functional assays, we could show that lectin-sugar interactions play a key role in host and pathogen interactions of a prototype isolate of the vaginal Lactobacillus microbiota.
authors.affiliation: KU Leuven, Centre of Microbial and Plant Genetics, Leuven, Belgium.|University of Antwerp, Department of Bioscience Engineering, Research Group Environmental Ecology and Applied Microbiology, Antwerp, Belgium.
authors.forename: Shweta
authors.initials: S
authors.lastname: Malik
authors.affiliation: KU Leuven, Centre of Microbial and Plant Genetics, Leuven, Belgium.|University of Antwerp, Department of Bioscience Engineering, Research Group Environmental Ecology and Applied Microbiology, Antwerp, Belgium.
authors.forename: Mariya I
authors.initials: MI
authors.lastname: Petrova
authors.affiliation: KU Leuven, Centre of Microbial and Plant Genetics, Leuven, Belgium.|University of Antwerp, Department of Bioscience Engineering, Research Group Environmental Ecology and Applied Microbiology, Antwerp, Belgium.
authors.forename: Nicole C E
authors.initials: NC
authors.lastname: Imholz
authors.affiliation: KU Leuven, Centre of Microbial and Plant Genetics, Leuven, Belgium.
authors.forename: Tine L A
authors.initials: TL
authors.lastname: Verhoeven
authors.affiliation: KU Leuven, Department of Microbiology and Immunology, Rega Institute for Medical Research, Laboratory of Virology and Chemotherapy, Leuven, Belgium.
authors.forename: Sam
authors.initials: S
authors.lastname: Noppen
authors.affiliation: Ghent University, Department of Molecular Biotechnology, Ghent, Belgium.
authors.forename: Els J M
authors.initials: EJ
authors.lastname: Van Damme
authors.affiliation: KU Leuven, Department of Microbiology and Immunology, Rega Institute for Medical Research, Laboratory of Virology and Chemotherapy, Leuven, Belgium.
authors.forename: Sandra
authors.initials: S
authors.lastname: Liekens
authors.affiliation: KU Leuven, Department of Microbiology and Immunology, Rega Institute for Medical Research, Laboratory of Virology and Chemotherapy, Leuven, Belgium.
authors.forename: Jan
authors.initials: J
authors.lastname: Balzarini
authors.affiliation: KU Leuven, Department of Microbiology and Immunology, Rega Institute for Medical Research, Laboratory of Virology and Chemotherapy, Leuven, Belgium.
authors.forename: Dominique
authors.initials: D
authors.lastname: Schols
authors.affiliation: KU Leuven, Centre of Microbial and Plant Genetics, Leuven, Belgium.
authors.forename: Jos
authors.initials: J
authors.lastname: Vanderleyden
authors.affiliation: KU Leuven, Centre of Microbial and Plant Genetics, Leuven, Belgium.|University of Antwerp, Department of Bioscience Engineering, Research Group Environmental Ecology and Applied Microbiology, Antwerp, Belgium.
authors.forename: Sarah
authors.initials: S
authors.lastname: Lebeer
mesh_terms: D000371:Agglutination; D001422:Bacterial Adhesion; D001426:Bacterial Proteins / Q000494:pharmacology / Q000502:physiology*; D018441:Biofilms; D002176:Candida albicans / Q000502:physiology; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D004847:Epithelial Cells / Q000382:microbiology; D005260:Female; D015699:HIV Envelope Protein gp120 / Q000737:chemistry; D015700:HIV Envelope Protein gp41 / Q000737:chemistry; D015497:HIV-1 / Q000502:physiology; D006801:Humans; D048191:Lactiplantibacillus plantarum / Q000502:physiology*; D008351:Mannans / Q000737:chemistry; D037601:Mannose-Binding Lectin / Q000494:pharmacology / Q000502:physiology*; D056265:Microbial Interactions; D011485:Protein Binding; D012441:Saccharomyces cerevisiae / Q000502:physiology; D012486:Salmonella typhimurium / Q000187:drug effects / Q000502:physiology; D014621:Vagina / Q000382:microbiology*
chemical_list: D001426:Bacterial Proteins; D015699:HIV Envelope Protein gp120; D015700:HIV Envelope Protein gp41; D008351:Mannans; D037601:Mannose-Binding Lectin