title: Comparative transcriptomic analyses of male and female adult Toxocara canis.
abstract: Toxocariasis is an important, neglected zoonosis caused mainly by Toxocara canis. Although our knowledge of helminth molecular biology is improving through completed draft genome projects, there is limited detailed information on the molecular biology of Toxocara species. Here, transcriptomic sequencing of male and female adult T. canis and comparative analyses were conducted. For each sex, two-thirds (66-67%) of quality-filtered reads mapped to the gene set of T. canis, and at least five reads mapped to each of 16,196 (87.1%) of all 18,596 genes, and 321 genes were specifically transcribed in female and 1467 in male T. canis. Genes differentially transcribed between the two sexes were identified, enriched biological processes and pathways linked to these genes established, and molecules associated with reproduction and development predicted. In addition, small RNA pathways involved in reproduction were characterized, but there was no evidence for piwi RNA pathways in adult T. canis. The results of this transcriptomic study should provide a useful basis to support investigations of the reproductive biology of T. canis and related nematodes.2.
authors.affiliation: Department of Veterinary Medicine, Rongchang Campus of Southwest University, Chongqing 402460, People's Republic of China. Electronic address: rongqiongzhou@126.com.
authors.forename: Rong-Qiong
authors.initials: RQ
authors.lastname: Zhou
authors.affiliation: Department of Veterinary Medicine, Rongchang Campus of Southwest University, Chongqing 402460, People's Republic of China; Faculty of Veterinary and Agricultural Sciences, The University of Melbourne, Parkville, Victoria 3010, Australia. Electronic address: guangxum@student.unimelb.edu.au.
authors.forename: Guang-Xu
authors.initials: GX
authors.lastname: Ma
authors.affiliation: Faculty of Veterinary and Agricultural Sciences, The University of Melbourne, Parkville, Victoria 3010, Australia. Electronic address: pasi.korhonen@unimelb.edu.au.
authors.forename: Pasi K
authors.initials: PK
authors.lastname: Korhonen
authors.affiliation: Department of Veterinary Medicine, Rongchang Campus of Southwest University, Chongqing 402460, People's Republic of China. Electronic address: 15823120635@163.com.
authors.forename: Yong-Li
authors.initials: YL
authors.lastname: Luo
authors.affiliation: Department of Veterinary Medicine, Rongchang Campus of Southwest University, Chongqing 402460, People's Republic of China. Electronic address: bbaacing@126.com.
authors.forename: Hong-Hong
authors.initials: HH
authors.lastname: Zhu
authors.affiliation: Department of Veterinary Medicine, Rongchang Campus of Southwest University, Chongqing 402460, People's Republic of China. Electronic address: realmyongfang@163.com.
authors.forename: Yong-Fang
authors.initials: YF
authors.lastname: Luo
authors.affiliation: Faculty of Veterinary and Agricultural Sciences, The University of Melbourne, Parkville, Victoria 3010, Australia. Electronic address: robinbg@unimelb.edu.au.
authors.forename: Robin B
authors.initials: RB
authors.lastname: Gasser
authors.affiliation: State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400715, People's Republic of China. Electronic address: xiaqy@swu.edu.cn.
authors.forename: Qing-You
authors.initials: QY
authors.lastname: Xia
mesh_terms: D000818:Animals; D005260:Female; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D017238:Genes, Helminth; D015801:Helminth Proteins / Q000235:genetics; D006801:Humans; D008297:Male; D034622:RNA Interference; D018510:RNA, Helminth / Q000235:genetics; D034741:RNA, Small Interfering / Q000235:genetics; D012098:Reproduction / Q000235:genetics; D012727:Sex Characteristics; D017166:Toxocara canis / Q000235:genetics* / Q000254:growth & development / Q000502:physiology; D059467:Transcriptome
chemical_list: D015801:Helminth Proteins; D018510:RNA, Helminth; D034741:RNA, Small Interfering
keywords: Development; Gender-enriched genes; Reproduction; Small RNA pathways; Toxocara canis; Transcriptome