title: Improved formulation of cationic solid lipid nanoparticles displays cellular uptake and biological activity of nucleic acids.
abstract: Non-viral delivery using cationic solid lipid nanoparticles (SLNs) represents a useful strategy to introduce large DNA and RNA molecules to target cells. A careful selection of components and their amounts is critical to improve transfection efficiency. In this work, a selected and optimized formulation of SLNs was used to efficiently transfect circular DNA and linear RNA molecules into cells. We characterized the main physicochemical characteristics and binding capabilities of these SLNs and show that they deliver DNA and RNA molecules into cells where they display full bioactivity at nontoxic concentrations using fluorescence- and luminescence-based methodologies. Hence, we established a novel and simple SLN formulation as a powerful tool for future therapeutic use.
authors.affiliation: Drug Development Service, Department of Pharmacy and Pharmaceutical Technology, Faculty of Pharmacy, University of Barcelona, Avda. Joan XXIII, s/n 08028 Barcelona, Spain.
authors.forename: Anna
authors.initials: A
authors.lastname: Fàbregas
authors.affiliation: Department of Molecular Biology, Institute of Parasitology and Biomedicine "López Neyra" (IPBLN-CSIC), PTS, Granada 18016, Spain.
authors.forename: Silvia
authors.initials: S
authors.lastname: Prieto-Sánchez
authors.affiliation: Drug Development Service, Department of Pharmacy and Pharmaceutical Technology, Faculty of Pharmacy, University of Barcelona, Avda. Joan XXIII, s/n 08028 Barcelona, Spain; Department of Molecular Biology, Institute of Parasitology and Biomedicine "López Neyra" (IPBLN-CSIC), PTS, Granada 18016, Spain.
authors.forename: Marc
authors.initials: M
authors.lastname: Suñé-Pou
authors.affiliation: Department of Molecular Biology, Institute of Parasitology and Biomedicine "López Neyra" (IPBLN-CSIC), PTS, Granada 18016, Spain.
authors.forename: Sofía
authors.initials: S
authors.lastname: Boyero-Corral
authors.affiliation: Drug Development Service, Department of Pharmacy and Pharmaceutical Technology, Faculty of Pharmacy, University of Barcelona, Avda. Joan XXIII, s/n 08028 Barcelona, Spain; Bellvitge Biomedical Research Institute (IDIBELL), University of Barcelona, Spain.
authors.forename: Josep Ramón
authors.initials: JR
authors.lastname: Ticó
authors.affiliation: Drug Development Service, Department of Pharmacy and Pharmaceutical Technology, Faculty of Pharmacy, University of Barcelona, Avda. Joan XXIII, s/n 08028 Barcelona, Spain; Bellvitge Biomedical Research Institute (IDIBELL), University of Barcelona, Spain.
authors.forename: Encarna
authors.initials: E
authors.lastname: García-Montoya
authors.affiliation: Drug Development Service, Department of Pharmacy and Pharmaceutical Technology, Faculty of Pharmacy, University of Barcelona, Avda. Joan XXIII, s/n 08028 Barcelona, Spain; Bellvitge Biomedical Research Institute (IDIBELL), University of Barcelona, Spain.
authors.forename: Pilar
authors.initials: P
authors.lastname: Pérez-Lozano
authors.affiliation: Drug Development Service, Department of Pharmacy and Pharmaceutical Technology, Faculty of Pharmacy, University of Barcelona, Avda. Joan XXIII, s/n 08028 Barcelona, Spain; Bellvitge Biomedical Research Institute (IDIBELL), University of Barcelona, Spain.
authors.forename: Montserrat
authors.initials: M
authors.lastname: Miñarro
authors.affiliation: Drug Development Service, Department of Pharmacy and Pharmaceutical Technology, Faculty of Pharmacy, University of Barcelona, Avda. Joan XXIII, s/n 08028 Barcelona, Spain; Bellvitge Biomedical Research Institute (IDIBELL), University of Barcelona, Spain.
authors.forename: Josep Mª
authors.initials: JM
authors.lastname: Suñé-Negre
authors.affiliation: Department of Cell Biology and Immunology, Institute of Parasitology and Biomedicine "López Neyra" (IPBLN-CSIC), PTS, Granada 18016, Spain.
authors.forename: Cristina
authors.initials: C
authors.lastname: Hernández-Munain
authors.affiliation: Department of Molecular Biology, Institute of Parasitology and Biomedicine "López Neyra" (IPBLN-CSIC), PTS, Granada 18016, Spain. Electronic address: csune@ipb.csic.es.
authors.forename: Carlos
authors.initials: C
authors.lastname: Suñé
mesh_terms: D002412:Cations / Q000737:chemistry; D002460:Cell Line; D002626:Chemistry, Pharmaceutical / Q000379:methods; D004247:DNA / Q000008:administration & dosage* / Q000235:genetics; D005453:Fluorescence; D018014:Gene Transfer Techniques; D006801:Humans; D008055:Lipids / Q000737:chemistry*; D008163:Luminescent Measurements; D053758:Nanoparticles; D012313:RNA / Q000008:administration & dosage* / Q000235:genetics; D014162:Transfection
chemical_list: D002412:Cations; D008055:Lipids; D012313:RNA; D004247:DNA
keywords: Binding efficiency; Cationic solid lipid nanoparticles (SLNs); Luciferase; Octadecylamine (PubChem ID: 15793); Plasmid DNA; Poloxamer 188 (PubChem CID: 24751); Stearic acid (PubChem CID: 5281); Transfection; siRNA