title: [Expression of PAK1 in bladder cancer and its influence on invasion of bladder cancer cells].
abstract: Objective:To investigate the expression of p21-activated kinase 1 (PAK1) in bladder cancer and its biological influence on invasion ability of bladder cancer cells.Methods:A total of 54 paraffin-embedded bladder cancer tissue samples and 12 normal bladder tissue specimens were retrieved in Jinshan Hospital Affiliated to Fudan University between January 2009 and December 2012. The expression of PAK1 in these tissues was detected by immunohistochemical staining. The PAK1 mRNA and protein levels were measured in bladder cancer cell lines and human normal bladder epithelial cell line using real-time, fluorescence-based quantitative polymerase chain reaction (RT-PCR) and Western blot, respectively. A stable PAK1 gene silencing bladder cancer cell strain 5637 were successfully constructed. After treatment with PAK1 RNA interference(RNAi), the ability of migration and invasion of the 5637 cell was evaluated using a Transwell system.Results:The expression of PAK1 proteins was significantly higher in bladder cancer tissues than in normal bladder tissues (28/54 vs 1/12,P<0.05). The overexpression of PAK1 was positively correlated with high histological grade, lymph node metastasis, and tumor size (allP<0.05). The mRNA level and protein level of PAK1 was much higher in bladder cancer cell lines T24, 5637 than human normal urothelial cell line SV-HUC-1.PCR and Western blot showed satisfactory inhibitory effect of PAK1 short hairpin RNA (shRNA) on PAK1 expression in 5637 bladder cancer cells. The number of PAK1 RNAi-treated 5637 cells traversed the membrane was decreased compared with the control group in migration and invasion assays.Conclusions:Overexpression of PAK1 in bladder cancer tissues may be an important feature of bladder cancer and related with the metastasis and invasion of bladder cancer. The molecular mechanisms involved in regulation of PAK1 expression needs further research.
authors.affiliation: Department of Urology, Clinical Medical College of Yangzhou University, Yangzhou 225001, China.
authors.forename: K
authors.initials: K
authors.lastname: Huang
authors.forename: G
authors.initials: G
authors.lastname: Chen
authors.forename: Y
authors.initials: Y
authors.lastname: Li
authors.forename: J K
authors.initials: JK
authors.lastname: Liu
authors.forename: Z Y
authors.initials: ZY
authors.lastname: Wang
authors.forename: G C
authors.initials: GC
authors.lastname: Zhou
mesh_terms: D015153:Blotting, Western; D006801:Humans; D008207:Lymphatic Metastasis; D034622:RNA Interference; D012333:RNA, Messenger; D034741:RNA, Small Interfering; D001749:Urinary Bladder Neoplasms; D054462:p21-Activated Kinases
chemical_list: D012333:RNA, Messenger; D034741:RNA, Small Interfering; C515903:PAK1 protein, human; D054462:p21-Activated Kinases