title: Direct Ethanol Production from Ionic Liquid-Pretreated Lignocellulosic Biomass by Cellulase-Displaying Yeasts.
abstract: Among the many types of lignocellulosic biomass pretreatment methods, the use of ionic liquids (ILs) is regarded as one of the most promising strategies. In this study, the effects of four kinds of ILs for pretreatment of lignocellulosic biomass such as bagasse, eucalyptus, and cedar were evaluated. In direct ethanol fermentation from biomass incorporated with ILs by cellulase-displaying yeast, 1-butyl-3-methylimidazolium acetate ([Bmim][OAc]) was the most effective IL. The ethanol production and yield from [Bmim][OAc]-pretreated bagasse reached 0.81 g/L and 73.4% of the theoretical yield after fermentation for 96 h. The results prove the initial concept, in which the direct fermentation from lignocellulosic biomass effectively promoted by the pretreatment with IL.
authors.affiliation: Organization of Advanced Science and Technology, Kobe University, 1-1 Rokkodaicho, Nada-ku, Kobe, Hyogo, 657-8501, Japan.|Department of Chemical Engineering, Osaka Prefecture University, 1-1 Gakuencho, Naka-ku, Sakai, Osaka, 599-8531, Japan.
authors.forename: Ryosuke
authors.initials: R
authors.lastname: Yamada
authors.affiliation: Organization of Advanced Science and Technology, Kobe University, 1-1 Rokkodaicho, Nada-ku, Kobe, Hyogo, 657-8501, Japan.|Division of Sustainable Resources Engineering, Faculty of Engineering, Hokkaido University, Kita 13, Nishi 8, Kita-Ku, Sapporo, 060-8628, Japan.
authors.forename: Kazunori
authors.initials: K
authors.lastname: Nakashima
authors.affiliation: Organization of Advanced Science and Technology, Kobe University, 1-1 Rokkodaicho, Nada-ku, Kobe, Hyogo, 657-8501, Japan.
authors.forename: Nanami
authors.initials: N
authors.lastname: Asai-Nakashima
authors.affiliation: Bio Fuel Group, Organic Material Engineering Division, Toyota Motor Corporation, Aichi, Japan.
authors.forename: Wataru
authors.initials: W
authors.lastname: Tokuhara
authors.affiliation: Biotechnology Laboratory, Toyota Central R&D Labs., Inc., Aichi, Japan.
authors.forename: Nobuhiro
authors.initials: N
authors.lastname: Ishida
authors.affiliation: Biotechnology Laboratory, Toyota Central R&D Labs., Inc., Aichi, Japan.
authors.forename: Satoshi
authors.initials: S
authors.lastname: Katahira
authors.affiliation: Center for Future Chemistry, Kyushu University, 744 Motooka, Fukuoka, 819-0395, Japan.|Department of Applied Chemistry, Graduate School of Engineering, Kyushu University, Fukuoka, Japan.
authors.forename: Noriho
authors.initials: N
authors.lastname: Kamiya
authors.affiliation: Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1-1 Rokkodaicho, Nada-ku, Kobe, Hyogo, 657-8501, Japan. ochiaki@port.kobe-u.ac.jp.
authors.forename: Chiaki
authors.initials: C
authors.lastname: Ogino
authors.affiliation: Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1-1 Rokkodaicho, Nada-ku, Kobe, Hyogo, 657-8501, Japan.
authors.forename: Akihiko
authors.initials: A
authors.lastname: Kondo
mesh_terms: D001230:Aspergillus / Q000187:drug effects / Q000201:enzymology; D056804:Biofuels; D018533:Biomass; D028203:Cedrus / Q000737:chemistry; D044602:Cellulases / Q000378:metabolism; D002482:Cellulose / Q000737:chemistry; D000431:Ethanol / Q000378:metabolism*; D005052:Eucalyptus / Q000737:chemistry; D005285:Fermentation; D005656:Fungal Proteins / Q000378:metabolism; D005947:Glucose / Q000096:biosynthesis*; D007093:Imidazoles / Q000737:chemistry* / Q000494:pharmacology; D052578:Ionic Liquids / Q000737:chemistry* / Q000494:pharmacology; D007700:Kinetics; D008031:Lignin / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000201:enzymology; D014242:Trichoderma / Q000187:drug effects / Q000201:enzymology; D014994:Xylose / Q000096:biosynthesis*
chemical_list: C525963:1-butyl-3-methylimidazolium; D056804:Biofuels; D005656:Fungal Proteins; D007093:Imidazoles; D052578:Ionic Liquids; C036909:lignocellulose; D000431:Ethanol; D002482:Cellulose; D008031:Lignin; C027433:bagasse; D014994:Xylose; D044602:Cellulases; D005947:Glucose
keywords: Direct fermentation; Ethanol; Ionic liquid; Lignocellulosic biomass; Yeast