title: A rapid and high-throughput method for the determination of serum uric acid based on microarray technology and nanomaterial.
abstract: Serum uric acid (SUA) is a new therapeutic target for non-alcoholic fatty liver disease (NAFLD). In this study, we introduced a chemiluminescence (CL) method combined with microarray technology and a simple fabrication procedure to obtain a highly sensitive SUA probe based on a mesoporous metal oxide nanomaterial. The high-throughput method was based on the generation of H2O2from SUA by immobilized uricase and its measurement by a CL reaction catalyzed by mesoporous metal oxide nanomaterials. The CL probe was designed for SUA The linear range of the uric acid concentration was 0.6-9 μM and the detection limit was 0.1 μM. In comparison with the other SUA detection techniques, this method has the advantages of a low detection limit, high sensitivity and simplicity. A new sensitive high-throughput approach was obtained for the determination of SUA.
authors.affiliation: School of Chemistry and Environmental Engineering, Shanxi Datong University, Datong, Shanxi, China.
authors.forename: Xiaohua
authors.initials: X
authors.lastname: Li
authors.affiliation: School of Chemistry and Environmental Engineering, Shanxi Datong University, Datong, Shanxi, China.
authors.forename: Yunfeng
authors.initials: Y
authors.lastname: Bai
authors.affiliation: School of Chemistry and Environmental Engineering, Shanxi Datong University, Datong, Shanxi, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Feng
authors.affiliation: School of Chemistry and Chemical Engineering, Shaanxi Normal University, China.
authors.forename: Zhujun
authors.initials: Z
authors.lastname: Zhang
mesh_terms: D000481:Allantoin / Q000378:metabolism; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods; D003035:Cobalt / Q000737:chemistry; D004800:Enzymes, Immobilized / Q000737:chemistry / Q000378:metabolism; D057166:High-Throughput Screening Assays; D006801:Humans; D057230:Limit of Detection; D008163:Luminescent Measurements / Q000295:instrumentation / Q000379:methods*; D008165:Luminol / Q000737:chemistry; D046228:Microarray Analysis / Q000295:instrumentation / Q000379:methods; D053758:Nanoparticles / Q000737:chemistry*; D065626:Non-alcoholic Fatty Liver Disease / Q000097:blood; D010087:Oxides / Q000737:chemistry; D012680:Sensitivity and Specificity; D012822:Silicon Dioxide / Q000737:chemistry; D013056:Spectrophotometry, Ultraviolet; D014503:Urate Oxidase / Q000737:chemistry / Q000378:metabolism; D014527:Uric Acid / Q000097:blood*
chemical_list: D004800:Enzymes, Immobilized; D010087:Oxides; D014527:Uric Acid; D000481:Allantoin; D003035:Cobalt; D008165:Luminol; D012822:Silicon Dioxide; D014503:Urate Oxidase; C060728:cobalt oxide
keywords: high-throughput; microarray technology; nanomaterial; serum uric acid