title: Embryonic alcohol exposure promotes long-term effects on cerebral glutamate transport of adult zebrafish.
abstract: Ethanol is a widely consumed substance throughout the world. During development it can substantially damage the human fetus, whereas the developing brain is particularly vulnerable. The brain damage induced by prenatal alcohol exposure may lead to a variety of long-lasting behavioral and neurochemical problems. However, there are no data concerning the effects of developmental ethanol exposure on the glutamatergic system, where extracellular glutamate acts as signaling molecule. Here we investigated the effect of ethanol exposure for 2h (concentrations of 0.0%, 0.1%, 0.25%, 0.50%, and 1.00%) in embryos at 24h post-fertilization (hpf) by measuring the functionality of glutamate transporters in the brain of adult (4 months) zebrafish. However, ethanol 0.1%, 0.25% and 0.50% decreased transport of glutamate to 81.96%, 60.65% and 45.91% respectively, when compared with the control group. Interestingly, 1.00% was able to inhibit the transport activity to 68.85%. In response to the embryonic alcohol exposure, we found impairment in the function of cerebral glutamate transport in adult fish, contributing to long-term alteration in the homeostasis glutamatergic signaling.
authors.affiliation: Programa de Pós-graduação em Bioquímica, Departamento de Bioquímica, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul, Rua Ramiro Barcelos 2600-Anexo, 90035-003, Porto Alegre, RS, Brazil.
authors.forename: Suelen
authors.initials: S
authors.lastname: Baggio
authors.affiliation: Programa de Pós-graduação em Bioquímica, Departamento de Bioquímica, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul, Rua Ramiro Barcelos 2600-Anexo, 90035-003, Porto Alegre, RS, Brazil.
authors.forename: Ben Hur
authors.initials: BH
authors.lastname: Mussulini
authors.affiliation: Programa de Pós-graduação em Bioquímica, Departamento de Bioquímica, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul, Rua Ramiro Barcelos 2600-Anexo, 90035-003, Porto Alegre, RS, Brazil.
authors.forename: Diogo Losch
authors.initials: DL
authors.lastname: de Oliveira
authors.affiliation: Programa de Pós-graduação em Bioquímica, Departamento de Bioquímica, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul, Rua Ramiro Barcelos 2600-Anexo, 90035-003, Porto Alegre, RS, Brazil.
authors.forename: Kamila Cagliari
authors.initials: KC
authors.lastname: Zenki
authors.affiliation: Programa de Pós-graduação em Bioquímica, Departamento de Bioquímica, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul, Rua Ramiro Barcelos 2600-Anexo, 90035-003, Porto Alegre, RS, Brazil.
authors.forename: Emerson
authors.initials: E
authors.lastname: Santos da Silva
authors.affiliation: Programa de Pós-Graduação em Ciências da Saúde, Universidade do Extremo Sul Catarinense - UNESC, Av. Universitária, 1105, Bairro Universitário, 88806-000, Criciúma, SC, Brazil. Electronic address: eduprico@gmail.com.
authors.forename: Eduardo Pacheco
authors.initials: EP
authors.lastname: Rico
mesh_terms: D000818:Animals; D001522:Behavior, Animal / Q000187:drug effects*; D001692:Biological Transport; D001921:Brain / Q000187:drug effects* / Q000378:metabolism; D004625:Embryo, Nonmammalian / Q000187:drug effects* / Q000378:metabolism; D000431:Ethanol / Q000494:pharmacology*; D018698:Glutamic Acid / Q000378:metabolism*; D013997:Time Factors; D015027:Zebrafish / Q000196:embryology
chemical_list: D000431:Ethanol; D018698:Glutamic Acid
keywords: Ethanol; Fetal alcohol syndrome disorder; Glutamate; Zebrafish