title: eIF4B stimulates eIF4A ATPase and unwinding activities by direct interaction through its 7-repeats region.
abstract: Eukaryotic translation initiation starts with binding of the eIF4F complex to the 5'-m7G cap of the mRNA. Recruitment of the 43S pre-initiation complex (PIC), formed by the 40S ribosomal subunit and other translation initiation factors, leads to formation of the 48S PIC that then scans the 5'-untranslated region (5'-UTR) toward the start codon. The eIF4F complex consists of eIF4E, the cap binding protein, eIF4A, a DEAD-box RNA helicase that is believed to unwind secondary structures in the 5'-UTR during scanning, and eIF4G, a scaffold protein that binds to both eIF4E and eIF4A. The ATPase and helicase activities of eIF4A are jointly stimulated by eIF4G and the translation initiation factor eIF4B. Yeast eIF4B mediates recruitment of the 43S PIC to the cap-bound eIF4F complex by interacting with the 40S subunit and possibly with eIF4A. However, a direct interaction between yeast eIF4A and eIF4B has not been demonstrated yet. Here we show that eIF4B binds to eIF4A in the presence of RNA and ADPNP, independent of the presence of eIF4G. A stretch of seven moderately conserved repeats, the r1-7 region, is responsible for complex formation, for modulation of the conformational energy landscape of eIF4A by eIF4B, and for stimulating the RNA-dependent ATPase- and ATP-dependent RNA unwinding activities of eIF4A. The isolated r1-7 region only slightly stimulates eIF4A conformational changes and activities, suggesting that communication of the repeats with other regions of eIF4B is required for full stimulation of eIF4A activity, for recruitment of the PIC to the mRNA and for translation initiation.
authors.affiliation: a University of Muenster, Institute for Physical Chemistry , Muenster , Germany.
authors.forename: Alexandra Zoi
authors.initials: AZ
authors.lastname: Andreou
authors.affiliation: a University of Muenster, Institute for Physical Chemistry , Muenster , Germany.
authors.forename: Ulf
authors.initials: U
authors.lastname: Harms
authors.affiliation: a University of Muenster, Institute for Physical Chemistry , Muenster , Germany.
authors.forename: Dagmar
authors.initials: D
authors.lastname: Klostermeier
mesh_terms: D020121:5' Untranslated Regions; D000251:Adenosine Triphosphatases / Q000378:metabolism*; D001665:Binding Sites; D004789:Enzyme Activation; D039601:Eukaryotic Initiation Factor-4A / Q000737:chemistry / Q000378:metabolism*; D039642:Eukaryotic Initiation Factors / Q000737:chemistry / Q000378:metabolism*; D046912:Multiprotein Complexes / Q000378:metabolism; D011485:Protein Binding; D054730:Protein Interaction Domains and Motifs; D020365:RNA Helicases / Q000378:metabolism; D012333:RNA, Messenger / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D012091:Repetitive Sequences, Nucleic Acid; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism
chemical_list: D020121:5' Untranslated Regions; D039642:Eukaryotic Initiation Factors; D046912:Multiprotein Complexes; D012333:RNA, Messenger; C031799:eIF-4B; D039601:Eukaryotic Initiation Factor-4A; D000251:Adenosine Triphosphatases; C027900:RNA-dependent ATPase; D020365:RNA Helicases
keywords: ATP hydrolysis; DEAD-box helicase; conformational change; eIF4A; regulation