title: Genome-wide association mapping of resistance to eyespot disease (Pseudocercosporella herpotrichoides) in European winter wheat (Triticum aestivum L.) and fine-mapping of Pch1.
abstract: Genotypes with recombination events in the Triticum ventricosum introgression on chromosome 7D allowed to fine-map resistance gene Pch1, the main source of eyespot resistance in European winter wheat cultivars. Eyespot (also called Strawbreaker) is a common and serious fungal disease of winter wheat caused by the necrotrophic fungi Oculimacula yallundae and Oculimacula acuformis (former name Pseudocercosporella herpotrichoides). A genome-wide association study (GWAS) for eyespot was performed with 732 microsatellite markers (SSR) and 7761 mapped SNP markers derived from the 90 K iSELECT wheat array using a panel of 168 European winter wheat varieties as well as three spring wheat varieties and phenotypic evaluation of eyespot in field tests in three environments. Best linear unbiased estimations (BLUEs) were calculated across all trials and ranged from 1.20 (most resistant) to 5.73 (most susceptible) with an average value of 4.24 and a heritability of H2= 0.91. A total of 108 SSR and 235 SNP marker-trait associations (MTAs) were identified by considering associations with a -log10(P value) ≥3.0. Significant MTAs for eyespot-score BLUEs were found on chromosomes 1D, 2A, 2D, 3D, 5A, 5D, 6A, 7A and 7D for the SSR markers and chromosomes 1B, 2A, 2B, 2D, 3B and 7D for the SNP markers. For 18 varieties (10.5%), a highly resistant phenotype was detected that was linked to the presence of the resistance gene Pch1 on chromosome 7D. The identification of genotypes with recombination events in the introgressed genomic segment from Triticum ventricosum harboring the Pch1 resistance gene on chromosome 7DL allowed the fine-mapping of this gene using additional SNP markers and a potential candidate gene Traes_7DL_973A33763 coding for a CC-NBS-LRR class protein was identified.
authors.affiliation: Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), 06466, Gatersleben, Germany.
authors.forename: Christine D
authors.initials: CD
authors.lastname: Zanke
authors.affiliation: Julius Kühn Institute (JKI), 38104, Brunswick, Germany.
authors.forename: Bernd
authors.initials: B
authors.lastname: Rodemann
authors.affiliation: Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), 06466, Gatersleben, Germany.|Institute of Precision Medicine, Xiangya Hospital, Central South University, Hunan Sheng, People's Republic of China.
authors.forename: Jie
authors.initials: J
authors.lastname: Ling
authors.affiliation: Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), 06466, Gatersleben, Germany.
authors.forename: Quddoos H
authors.initials: QH
authors.lastname: Muqaddasi
authors.affiliation: TraitGenetics GmbH, 06466, Gatersleben, Germany.
authors.forename: Jörg
authors.initials: J
authors.lastname: Plieske
authors.affiliation: TraitGenetics GmbH, 06466, Gatersleben, Germany.
authors.forename: Andreas
authors.initials: A
authors.lastname: Polley
authors.affiliation: KWS LOCHOW GmbH, 29303, Bergen, Germany.
authors.forename: Sonja
authors.initials: S
authors.lastname: Kollers
authors.affiliation: KWS LOCHOW GmbH, 29303, Bergen, Germany.
authors.forename: Erhard
authors.initials: E
authors.lastname: Ebmeyer
authors.affiliation: KWS LOCHOW GmbH, 29303, Bergen, Germany.
authors.forename: Viktor
authors.initials: V
authors.lastname: Korzun
authors.affiliation: Syngenta France S.A.S., 78910, Orgerus, France.
authors.forename: Odile
authors.initials: O
authors.lastname: Argillier
authors.affiliation: Syngenta Seeds GmbH, 32107, Bad Salzuflen, Germany.
authors.forename: Gunther
authors.initials: G
authors.lastname: Stiewe
authors.affiliation: Syngenta Seeds GmbH, 32107, Bad Salzuflen, Germany.
authors.forename: Thomas
authors.initials: T
authors.lastname: Zschäckel
authors.affiliation: TraitGenetics GmbH, 06466, Gatersleben, Germany.
authors.forename: Martin W
authors.initials: MW
authors.lastname: Ganal
authors.affiliation: Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), 06466, Gatersleben, Germany. roder@ipk-gatersleben.de.
authors.forename: Marion S
authors.initials: MS
authors.lastname: Röder
mesh_terms: D001203:Ascomycota; D002874:Chromosome Mapping; D060467:Disease Resistance / Q000235:genetics*; D017343:Genes, Plant; D056726:Genetic Association Studies; D005819:Genetic Markers; D005838:Genotype; D018895:Microsatellite Repeats; D010641:Phenotype; D010935:Plant Diseases / Q000235:genetics* / Q000382:microbiology; D020641:Polymorphism, Single Nucleotide; D014908:Triticum / Q000235:genetics* / Q000382:microbiology
chemical_list: D005819:Genetic Markers