title: Sequence analysis of the gliding protein Gli349 inMycoplasma mobile.
abstract: The motile mechanism ofMycoplasma mobileremains unknown but is believed to differ from any previously identified mechanism in bacteria. Gli349 ofM. mobileis known to be responsible for both adhesion to glass surfaces and mobility. We therefore carried out sequence analyses of Gli349 and its homolog MYPU2110 fromM. pulmonisto decipher their structures. We found that the motif "YxxxxxGF" appears 11 times in Gli349 and 16 times in MYPU2110. Further analysis of the sequences revealed that Gli349 contains 18 repeats of about 100 amino acid residues each, and MYPU2110 contains 22. No sequence homologous to any of the repeats was found in the NCBI RefSeq non-redundant sequence database, and no compatible fold structure was found among known protein structures, suggesting that the repeat found in Gli349 and MYPU2110 is novel and takes a new fold structure. Proteolysis of Gli349 using chymotrypsin revealed that cleavage positions were often located between the repeats, implying that regions connecting repeats are unstructured, flexible and exposed to the solvent. Assuming that each repeat folds into a structural domain, we constructed a model of Gli349 that fits well the shape and size of images obtained with electron microscopy.
authors.affiliation: Graduate School of Information Science, Nara Institute of Science and Technology, 8916-5, Takayama-cho, Ikoma, Nara 630-0101, Japan; Center for Promotion of Computational Science and Engineering, Japan Atomic Energy Research Institute, 8-1, Umemidai, Soraku-gun, Kyoto 619-0215, Japan.
authors.forename: Shoichi
authors.initials: S
authors.lastname: Metsugi
authors.affiliation: Graduate School of Science, Osaka City University, 3-3-138, Sugimoto, Sumiyoshi-ku, Osaka 558-8585, Japan.
authors.forename: Atsuko
authors.initials: A
authors.lastname: Uenoyama
authors.affiliation: Graduate School of Science, Osaka City University, 3-3-138, Sugimoto, Sumiyoshi-ku, Osaka 558-8585, Japan.
authors.forename: Jun
authors.initials: J
authors.lastname: Adan-Kubo
authors.affiliation: Graduate School of Science, Osaka City University, 3-3-138, Sugimoto, Sumiyoshi-ku, Osaka 558-8585, Japan; PRESTO, Japan Science and Technology Agency.
authors.forename: Makoto
authors.initials: M
authors.lastname: Miyata
authors.affiliation: Center for Promotion of Computational Science and Engineering, Japan Atomic Energy Research Institute, 8-1, Umemidai, Soraku-gun, Kyoto 619-0215, Japan; CREST, Japan Science and Technology Agency.
authors.forename: Kei
authors.initials: K
authors.lastname: Yura
authors.affiliation: Center for Promotion of Computational Science and Engineering, Japan Atomic Energy Research Institute, 8-1, Umemidai, Soraku-gun, Kyoto 619-0215, Japan; PRESTO, Japan Science and Technology Agency; Neutron Science Research Center, Japan Atomic Energy Research Institute, 8-1, Umemidai, Souraku-gun, Kyoto 619-0215, Japan.
authors.forename: Hidetoshi
authors.initials: H
authors.lastname: Kono
authors.affiliation: Graduate School of Information Science, Nara Institute of Science and Technology, 8916-5, Takayama-cho, Ikoma, Nara 630-0101, Japan; Center for Promotion of Computational Science and Engineering, Japan Atomic Energy Research Institute, 8-1, Umemidai, Soraku-gun, Kyoto 619-0215, Japan; Neutron Science Research Center, Japan Atomic Energy Research Institute, 8-1, Umemidai, Souraku-gun, Kyoto 619-0215, Japan.
authors.forename: Nobuhiro
authors.initials: N
authors.lastname: Go
keywords: Gli349; Mycoplasma mobile; YGF motif; motility; repeat sequence; sequence analysis