title: The effect of green tea polyphenols on gut microbial diversity and fat deposition in C57BL/6J HFA mice.
abstract: Quantitative and qualitative changes in gut microbial composition have been linked to obesity and obesity-related complications, and eating pattern has been shown to significantly impact the gut microbiome. Meanwhile, tea polyphenols are known to have health benefits such as improving glucose tolerance and decreasing liver fat deposition that may be helpful in combating obesity and obesity-related disorders. We therefore studied the effect of green tea polyphenols on gut microbial diversity and fat deposition in C57BL/6J Human Flora-Associated (HFA) mice, which were divided into five groups: low fat (LF), high fat (HF), high fat + 0.05% tea polyphenols (HF + 0.05% TP), high fat + 0.2% tea polyphenols (HF + 0.2% TP) and high fat + 0.8% tea polyphenols (HF + 0.8% TP). 16S rRNA V6-V8 region PCR-DGGE profiles showed that a high fat diet was associated with a significant reduction in microbial diversity. This reduction could be alleviated by a HF + 0.2% TP diet, with a significant increase in the number of lactic acid bacteria in the HF + 0.2% TP group compared with the LF group (P < 0.05). Body weight (P < 0.05) and fat pad weight (P < 0.001) were significantly increased in the HF compared with the LF group, with notable adipocyte hypertrophy in the HF group, indicating successful establishment of the high fat model. Body weight among the HF + 0.2% TP group and HF + 0.8% TP group (but not the HF + 0.05% TP group) was significantly lower than the body weight in the HF group (P < 0.01). Therefore, tea polyphenols may effectively retard diet-induced weight gain and body fat gain, adipocyte hypertrophy and hepatic steatosis in a dose-dependent manner.
authors.affiliation: College of Food Science, South China Agricultural University, Guangzhou 510642, China. fxiang@scau.edu.cn and Guangdong Province Key Laboratory for Green Processing of Natural Products and Product Safety, Guangzhou 510642, China.
authors.forename: Li
authors.initials: L
authors.lastname: Wang
authors.affiliation: Department of Laboratory Animal Science, College of Basic Medical Sciences, Third Military Medical University, Chongqing 40038, China.
authors.forename: Benhua
authors.initials: B
authors.lastname: Zeng
authors.affiliation: College of Food Science, South China Agricultural University, Guangzhou 510642, China. fxiang@scau.edu.cn.
authors.forename: Xiaojing
authors.initials: X
authors.lastname: Zhang
authors.affiliation: College of Food Science, South China Agricultural University, Guangzhou 510642, China. fxiang@scau.edu.cn.
authors.forename: Zhenlin
authors.initials: Z
authors.lastname: Liao
authors.affiliation: College of Food Science, South China Agricultural University, Guangzhou 510642, China. fxiang@scau.edu.cn.
authors.forename: Lihui
authors.initials: L
authors.lastname: Gu
authors.affiliation: College of Food Science, South China Agricultural University, Guangzhou 510642, China. fxiang@scau.edu.cn.
authors.forename: Zhiwei
authors.initials: Z
authors.lastname: Liu
authors.affiliation: College of Food Science, South China Agricultural University, Guangzhou 510642, China. fxiang@scau.edu.cn.
authors.forename: Qingping
authors.initials: Q
authors.lastname: Zhong
authors.affiliation: Department of Laboratory Animal Science, College of Basic Medical Sciences, Third Military Medical University, Chongqing 40038, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Wei
authors.affiliation: College of Food Science, South China Agricultural University, Guangzhou 510642, China. fxiang@scau.edu.cn.
authors.forename: Xiang
authors.initials: X
authors.lastname: Fang
mesh_terms: D000818:Animals; D004041:Dietary Fats / Q000008:administration & dosage* / Q000009:adverse effects; D005234:Fatty Liver / Q000517:prevention & control*; D000069196:Gastrointestinal Microbiome / Q000187:drug effects*; D051379:Mice; D008810:Mice, Inbred C57BL; D059808:Polyphenols / Q000737:chemistry / Q000494:pharmacology*; D013662:Tea / Q000737:chemistry*
chemical_list: D004041:Dietary Fats; D059808:Polyphenols; D013662:Tea