title: TCRα-TCRβ pairing controls recognition of CD1d and directs the development of adipose NKT cells.
abstract: The interaction between the T cell antigen receptor (TCR) expressed by natural killer T cells (NKT cells) and the antigen-presenting molecule CD1d is distinct from interactions between the TCR and major histocompatibility complex (MHC). Our molecular modeling suggested that a hydrophobic patch created after TCRα-TCRβ pairing has a role in maintaining the conformation of the NKT cell TCR. Disruption of this patch ablated recognition of CD1d by the NKT cell TCR but not interactions of the TCR with MHC. Partial disruption of the patch, while permissive to the recognition of CD1d, significantly altered NKT cell development, which resulted in the selective accumulation of adipose-tissue-resident NKT cells. These results indicate that a key component of the TCR is essential for the development of a distinct population of NKT cells.
authors.affiliation: Child Health Institute of New Jersey, Rutgers University, New Brunswick, New Jersey, USA.|Rutgers Robert Wood Johnson Medical School, Rutgers University, New Brunswick, New Jersey, USA.
authors.forename: Joshua A
authors.initials: JA
authors.lastname: Vieth
authors.affiliation: Immunology Program, Sloan-Kettering Institute, Memorial Sloan-Kettering Cancer Center, New York, New York, USA.
authors.forename: Joy
authors.initials: J
authors.lastname: Das
authors.affiliation: Child Health Institute of New Jersey, Rutgers University, New Brunswick, New Jersey, USA.|Rutgers Robert Wood Johnson Medical School, Rutgers University, New Brunswick, New Jersey, USA.
authors.forename: Fanomezana M
authors.initials: FM
authors.lastname: Ranaivoson
authors.affiliation: Child Health Institute of New Jersey, Rutgers University, New Brunswick, New Jersey, USA.|Rutgers Robert Wood Johnson Medical School, Rutgers University, New Brunswick, New Jersey, USA.|Department of Neuroscience and Cell Biology, Rutgers University, New Brunswick, New Jersey, USA.
authors.forename: Davide
authors.initials: D
authors.lastname: Comoletti
authors.affiliation: Child Health Institute of New Jersey, Rutgers University, New Brunswick, New Jersey, USA.|Rutgers Robert Wood Johnson Medical School, Rutgers University, New Brunswick, New Jersey, USA.|Rutgers Graduate School of Biomedical Sciences, Rutgers University, New Brunswick, New Jersey, USA.|Department of Pediatrics, Rutgers University, New Brunswick, New Jersey, USA.|Department of Pharmacology, Rutgers University, New Brunswick, New Jersey, USA.
authors.forename: Lisa K
authors.initials: LK
authors.lastname: Denzin
authors.affiliation: Child Health Institute of New Jersey, Rutgers University, New Brunswick, New Jersey, USA.|Rutgers Robert Wood Johnson Medical School, Rutgers University, New Brunswick, New Jersey, USA.|Rutgers Graduate School of Biomedical Sciences, Rutgers University, New Brunswick, New Jersey, USA.|Department of Pediatrics, Rutgers University, New Brunswick, New Jersey, USA.|Department of Pharmacology, Rutgers University, New Brunswick, New Jersey, USA.
authors.forename: Derek B
authors.initials: DB
authors.lastname: Sant'Angelo
mesh_terms: D000273:Adipose Tissue / Q000276:immunology*; D000818:Animals; D017951:Antigen Presentation; D055610:Antigens, CD1d / Q000378:metabolism*; D002454:Cell Differentiation / Q000235:genetics; D002478:Cells, Cultured; D003198:Computer Simulation; D015395:Histocompatibility Antigens Class I / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D055611:Natural Killer T-Cells / Q000502:physiology*; D011485:Protein Binding; D011487:Protein Conformation; D015202:Protein Engineering; D055503:Protein Multimerization; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000235:genetics / Q000378:metabolism*; D016176:T-Lymphocyte Subsets / Q000502:physiology*
chemical_list: D055610:Antigens, CD1d; D015395:Histocompatibility Antigens Class I; D016693:Receptors, Antigen, T-Cell, alpha-beta