title: Augmentation and repair of tendons using demineralised cortical bone.
abstract: In severe injuries with loss of tendon substance a tendon graft or a synthetic substitute is usually used to restore functional length. This is usually associated with donor site morbidity, host tissue reactions and lack of remodelling of the synthetic substitutes, which may result in suboptimal outcome. A biocompatible graft with mechanical and structural properties that replicate those of normal tendon and ligament has so far not been identified. The use of demineralised bone for tendon reattachment onto bone has been shown to be effective in promoting the regeneration of a normal enthesis. Because of its properties, we proposed that Demineralised Cortical Bone (DCB) could be used in repair of a large tendon defect.
  
  
  
  Allogenic DCB grafts in strip form were prepared from sheep cortical bone by acid decalcification and used to replace the enthesis and distal 1 cm of the ovine patellar tendon adjacent to the tibial tuberosity. In 6 animals the DCB strip was used to bridge the gap between the resected end of the tendon and was attached with bone anchors. Force plate analysis was done for each animal preoperatively and at weeks 3, 9, and 12 post operatively. At week 12, after euthanasia x-rays were taken and range of movements were recorded for hind limbs of each animal. Patella, patellar tendon - DCB and proximal tibia were harvested as a block and pQCT scan was done prior to histological analysis.
  
  
  
  Over time functional weight bearing significantly increased from 44% at 3 weeks post surgery to 79% at week 12. On retrieval none of the specimens showed any evidence of ossification of the DCB. Histological analysis proved formation of neo-enthesis with presence of fibrocartilage and mineralised fibrocartilage in all the specimens. DCB grafts contained host cells and showed evidence of vascularisation. Remodelling of the collagen leading to ligamentisation of the DCB was proved by the presence of crimp in the DCB graft on polarized microscopy.
  
  
  
  Combined with the appropriate surgical techniques, DCB can be used to achieve early mobilization and regeneration of a tendon defect which may be applicable to the repair of chronic rotator cuff injury in humans.
authors.affiliation: John Scales Centre for Biomedical Engineering, Institute of Orthopaedics and Musculo-Skeletal Science, University College London, Brockley Hill, Stanmore, Middlesex, HA7 4LP, United Kingdom.
authors.forename: Sherif
authors.identifier: 0000-0001-5597-2331
authors.initials: S
authors.lastname: Elnikety
authors.affiliation: John Scales Centre for Biomedical Engineering, Institute of Orthopaedics and Musculo-Skeletal Science, University College London, Brockley Hill, Stanmore, Middlesex, HA7 4LP, United Kingdom.
authors.forename: Catherine J
authors.initials: CJ
authors.lastname: Pendegrass
authors.affiliation: John Scales Centre for Biomedical Engineering, Institute of Orthopaedics and Musculo-Skeletal Science, University College London, Brockley Hill, Stanmore, Middlesex, HA7 4LP, United Kingdom.
authors.forename: Roberta Ferro
authors.initials: RF
authors.lastname: de Godoy
authors.affiliation: John Scales Centre for Biomedical Engineering, Institute of Orthopaedics and Musculo-Skeletal Science, University College London, Brockley Hill, Stanmore, Middlesex, HA7 4LP, United Kingdom.
authors.forename: Charles
authors.initials: C
authors.lastname: Holden
authors.affiliation: John Scales Centre for Biomedical Engineering, Institute of Orthopaedics and Musculo-Skeletal Science, University College London, Brockley Hill, Stanmore, Middlesex, HA7 4LP, United Kingdom. g.blunn@ucl.ac.uk.
authors.forename: Gordon W
authors.initials: GW
authors.lastname: Blunn
mesh_terms: D000818:Animals; D018775:Bone Demineralization Technique; D000071538:Cortical Bone; D005260:Female; D005684:Gait; D017847:Patellar Ligament / Q000000981:diagnostic imaging / Q000293:injuries / Q000601:surgery*; D011859:Radiography; D012756:Sheep; D013708:Tendon Injuries / Q000601:surgery*; D054457:Tissue Scaffolds; D016896:Treatment Outcome
keywords: Demineralised bone; Ligament; Scaffold; Tendon; Tissue engineering