title: [Expression of miR-let-7e-3p in cervical intraepithelial neoplasm and cervix carcinoma and its clinical significance].
abstract: Objective:To investigate the expression of microRNA (miRNA, miR) let-7e-3p in different cervical lesions and its clinical significance.Methods:The expression of miR-let-7e-3p in the tissues of normal cervix (n=26), high-grade squamous intraepithelial lesion (HSIL) (n=37), and cervix carcinoma (n=101) were detected by reverse transcription and quantitative polymerase chain reaction (RT-qPCR). The correlation of miR-let-7e-3p expression with the clinicopathological parameters of patients with cervical cancer was analyzed. miR-let-7e-3p mimic was transfected into cervical carcinoma Siha cells. The cell cycle and apoptosis were determined by flow cytometry; cell proliferation was determined by CCK-8 kit; and the migration and invasion of cells were determined by Transwell assay.Results:The relative expression levels of miR-let-7e-3p in normal cervix, HSIL, and cervical carcinoma were 1.45±0.24, 0.79±0.05 and 0.46±0.04, respectively (allP<0.05). After transfection with miR-let-7e-3p mimic, the S-phase fraction and apoptosis rate of Siha cells were increased significantly compared with control group[(29.76±6.6)% vs (13.38±1.3)%,P<0.05; (5.98±1.38)% vs (3.53±0.79)%,P<0.05, respectively]. OD of transfected Siha cells at 48, 72 and 96 h were 0.57±0.11,0.65±0.04 and 0.84±0.14, which were significantly lower than those of untransfected Siha cells (0.74±0.05, 0.93±0.10 and 1.47±0.14, allP<0.05). The migration and invasion abilities of transfected Siha cells were not significantly changed (allP>0.05).Conclusion:The expression of miR-let-7e-3p is down-regulated in cervical neoplasms, which is associated with cell cycle arrest and proliferation inhibition of cervical cancer cells.
authors.affiliation: Central Laboratory, Women's Hospital, Zhejiang University School of Medicine, Key Laboratory of Women's Reproductive Health of Zhejiang Province, Hangzhou 310006, China.
authors.forename: Xiaojing
authors.initials: X
authors.lastname: Chen
authors.affiliation: Central Laboratory, Women's Hospital, Zhejiang University School of Medicine, Key Laboratory of Women's Reproductive Health of Zhejiang Province, Hangzhou 310006, China.
authors.forename: Junfen
authors.initials: J
authors.lastname: Xu
authors.affiliation: Central Laboratory, Women's Hospital, Zhejiang University School of Medicine, Key Laboratory of Women's Reproductive Health of Zhejiang Province, Hangzhou 310006, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Ye
authors.affiliation: Central Laboratory, Women's Hospital, Zhejiang University School of Medicine, Key Laboratory of Women's Reproductive Health of Zhejiang Province, Hangzhou 310006, China.
authors.forename: Xiaodong
authors.initials: X
authors.lastname: Cheng
authors.affiliation: Central Laboratory, Women's Hospital, Zhejiang University School of Medicine, Key Laboratory of Women's Reproductive Health of Zhejiang Province, Hangzhou 310006, China.
authors.forename: Xing
authors.initials: X
authors.lastname: Xie
authors.affiliation: Central Laboratory, Women's Hospital, Zhejiang University School of Medicine, Key Laboratory of Women's Reproductive Health of Zhejiang Province, Hangzhou 310006, China. lbwg@zju.edu.cn.
authors.forename: Weiguo
authors.initials: W
authors.lastname: Lyu
mesh_terms: D017209:Apoptosis / Q000187:drug effects / Q000235:genetics; D002277:Carcinoma / Q000737:chemistry / Q000235:genetics; D002453:Cell Cycle / Q000187:drug effects / Q000235:genetics; D045744:Cell Line, Tumor / Q000737:chemistry* / Q000187:drug effects* / Q000502:physiology*; D002465:Cell Movement / Q000187:drug effects / Q000235:genetics; D049109:Cell Proliferation / Q000187:drug effects / Q000235:genetics; D015536:Down-Regulation / Q000502:physiology; D005260:Female; D006801:Humans; D035683:MicroRNAs / Q000032:analysis* / Q000494:pharmacology*; D009361:Neoplasm Invasiveness / Q000235:genetics / Q000503:physiopathology; D009385:Neoplastic Processes; D060888:Real-Time Polymerase Chain Reaction; D014162:Transfection; D002583:Uterine Cervical Neoplasms / Q000737:chemistry* / Q000235:genetics* / Q000503:physiopathology*; D002578:Uterine Cervical Dysplasia / Q000737:chemistry* / Q000235:genetics* / Q000503:physiopathology*
chemical_list: D035683:MicroRNAs