title: Cytomegalovirus Reinfections Stimulate CD8 T-Memory Inflation.
abstract: Cytomegalovirus (CMV) has been shown to induce large populations of CD8 T-effector memory cells that unlike central memory persist in large quantities following infection, a phenomenon commonly termed "memory inflation". Although murine models to date have shown very large and persistent CMV-specific T-cell expansions following infection, there is considerable variability in CMV-specific T-memory responses in humans. Historically such memory inflation in humans has been assumed a consequence of reactivation events during the life of the host. Because basic information about CMV infection/re-infection and reactivation in immune competent humans is not available, we used a murine model to test how primary infection, reinfection, and reactivation stimuli influence memory inflation. We show that low titer infections induce "partial" memory inflation of both mCMV specific CD8 T-cells and antibody. We show further that reinfection with different strains can boost partial memory inflation. Finally, we show preliminary results suggesting that a single strong reactivation stimulus does not stimulate memory inflation. Altogether, our results suggest that while high titer primary infections can induce memory inflation, reinfections during the life of a host may be more important than previously appreciated.
authors.affiliation: Department of Surgery, The Ohio State University, Columbus, OH 43210, United States of America.
authors.forename: Joanne
authors.initials: J
authors.lastname: Trgovcich
authors.affiliation: Department of Surgery, The Ohio State University, Columbus, OH 43210, United States of America.
authors.forename: Michelle
authors.initials: M
authors.lastname: Kincaid
authors.affiliation: Department of Surgery, The Ohio State University, Columbus, OH 43210, United States of America.
authors.forename: Alicia
authors.initials: A
authors.lastname: Thomas
authors.affiliation: Department of Surgery, Beth Israel Deaconess Medical Center / Harvard Medical School, Boston MA 02215, United States of America.
authors.forename: Marion
authors.initials: M
authors.lastname: Griessl
authors.affiliation: Department of Surgery, The Ohio State University, Columbus, OH 43210, United States of America.
authors.forename: Peter
authors.initials: P
authors.lastname: Zimmerman
authors.affiliation: Department of Surgery, The Ohio State University, Columbus, OH 43210, United States of America.
authors.forename: Varun
authors.initials: V
authors.lastname: Dwivedi
authors.affiliation: College of Veterinary Medicine, The Ohio State University, Columbus, OH 43210, United States of America.
authors.forename: Valerie
authors.initials: V
authors.lastname: Bergdall
authors.affiliation: Department of Medicine, Oxford University, Oxford, UK.
authors.forename: Paul
authors.initials: P
authors.lastname: Klenerman
authors.affiliation: Department of Surgery, Beth Israel Deaconess Medical Center / Harvard Medical School, Boston MA 02215, United States of America.
authors.forename: Charles H
authors.identifier: http://orcid.org/0000-0003-4446-3616
authors.initials: CH
authors.lastname: Cook
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D005260:Female; D006566:Herpesviridae Infections / Q000276:immunology*; D007156:Immunologic Memory; D051379:Mice; D008807:Mice, Inbred BALB C; D018448:Models, Immunological; D018146:Muromegalovirus / Q000276:immunology*
chemical_list: D000914:Antibodies, Viral