title: Identification and structure-activity relationship of purine derivatives as novel MTH1 inhibitors.
abstract: The human mutT homolog-1 (MTH1) protein prevents the incorporation of oxidized nucleotides such as 2-OH-dATP and 8-oxo-dGTP during DNA replication by hydrolyzing them into their corresponding monophosphates. It was found previously that cancer cells could tolerate oxidative stress due to this enzymatic activity of MTH1 and its inhibition could be a promising approach to treat several types of cancer. This finding has been challenged recently with increasing line of evidence suggesting that the cancer cell-killing effects of MTH1 inhibitors may be related to their engagement of off-targets. We have previously reported a few purine-based MTH1 inhibitors that enabled us to elucidate the dispensability of MTH1 in cancer cell survival. Here, we provide a detailed process of the identification of purine-based MTH1 inhibitors. Several new compounds with potency in the submicromolar range are disclosed. Furthermore, the structure-activity relationship and associated binding mode prediction using molecular docking have provided insights for the development of highly potent MTH1 inhibitors.
authors.affiliation: Structural Bioinformatics Team, RIKEN Center for Life Science Technologies, Yokohama, Kanagawa, Japan.
authors.forename: Ashutosh
authors.initials: A
authors.lastname: Kumar
authors.affiliation: Chemical Biology Research Group, RIKEN Center for Sustainable Resource Science, Wako, Saitama, Japan.
authors.forename: Tatsuro
authors.initials: T
authors.lastname: Kawamura
authors.affiliation: Chemical Biology Research Group, RIKEN Center for Sustainable Resource Science, Wako, Saitama, Japan.
authors.forename: Makoto
authors.initials: M
authors.lastname: Kawatani
authors.affiliation: Chemical Biology Research Group, RIKEN Center for Sustainable Resource Science, Wako, Saitama, Japan.
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Osada
authors.affiliation: Structural Bioinformatics Team, RIKEN Center for Life Science Technologies, Yokohama, Kanagawa, Japan.
authors.forename: Kam Y J
authors.initials: KYJ
authors.lastname: Zhang
mesh_terms: D000970:Antineoplastic Agents / Q000737:chemistry / Q000494:pharmacology; D001665:Binding Sites; D045643:DNA Repair Enzymes / Q000037:antagonists & inhibitors*; D004791:Enzyme Inhibitors / Q000737:chemistry / Q000494:pharmacology; D006801:Humans; D008958:Models, Molecular; D062105:Molecular Docking Simulation; D010744:Phosphoric Monoester Hydrolases / Q000037:antagonists & inhibitors*; D011687:Purines / Q000737:chemistry* / Q000494:pharmacology*; D013329:Structure-Activity Relationship
chemical_list: D000970:Antineoplastic Agents; D004791:Enzyme Inhibitors; C000624342:NPD15095; D011687:Purines; D010744:Phosphoric Monoester Hydrolases; C078207:8-oxodGTPase; D045643:DNA Repair Enzymes
keywords: MTH1; cancer; molecular docking; purines; structure-activity relationship