title: Generation of mouse lines with conditionally or constitutively inactivated Snca gene and Rosa26-stop-lacZ reporter located in cis on the mouse chromosome 6.
abstract: α-Synuclein is involved in many important molecular processes in neuronal cells and their synapses, and its malfunction has been linked to the development of Parkinson's and certain other neurodegenerative diseases. Animal models allowing tightly monitored conditional inactivation of the encoding gene, Snca, are indispensible for studies aimed at understanding normal function of α-synuclein in various neuronal populations and its role in pathogenesis of neurodegenerative diseases. We have recently reported the production of several novel mouse lines for manipulating expression of the endogenous Snca gene, including a line for Cre-recombinase-driven conditional inactivation of the gene (mice with floxed Snca) and a new line with a constitutive knockout of α-synuclein. Rosa26-stop-lacZ reporter cassette is commonly used for monitoring efficiency of Cre-recombination but in mouse genome Snca and Rosa26 loci are located on the same chromosome. Here we describe production of lines with a modified Snca locus, either floxed or constitutively inactivated and the Rosa26-stop-lacZ reporter cassette located in cis on the mouse chromosome 6. These new mouse lines are invaluable for fast identification of cells with inactivation of Snca by Cre-recombination and represent useful tools for in vivo studies of α-synuclein function and dysfunction.
authors.affiliation: Institute of Physiologically Active Compounds, Russian Academy of Sciences, 1 Severniy proezd, Chernogolovka, Moscow Region, Russian Federation, 142432.|Inserm, CRO2 UMR_S 911, Faculté de Pharmacie, Aix-Marseille Université, 13385, Marseille, France.
authors.forename: Andrei Yu
authors.initials: AY
authors.lastname: Roman
authors.affiliation: School of Biosciences, Cardiff University, Museum Avenue, Cardiff, CF10 3AX, UK.
authors.forename: Galina
authors.initials: G
authors.lastname: Limorenko
authors.affiliation: Institute of Physiologically Active Compounds, Russian Academy of Sciences, 1 Severniy proezd, Chernogolovka, Moscow Region, Russian Federation, 142432.
authors.forename: Alexey A
authors.initials: AA
authors.lastname: Ustyugov
authors.affiliation: Institute of Physiologically Active Compounds, Russian Academy of Sciences, 1 Severniy proezd, Chernogolovka, Moscow Region, Russian Federation, 142432.|School of Biosciences, Cardiff University, Museum Avenue, Cardiff, CF10 3AX, UK.
authors.forename: Tatiana V
authors.initials: TV
authors.lastname: Tarasova
authors.affiliation: Institute of Physiologically Active Compounds, Russian Academy of Sciences, 1 Severniy proezd, Chernogolovka, Moscow Region, Russian Federation, 142432.
authors.forename: Ekaterina A
authors.initials: EA
authors.lastname: Lysikova
authors.affiliation: Institute of Physiologically Active Compounds, Russian Academy of Sciences, 1 Severniy proezd, Chernogolovka, Moscow Region, Russian Federation, 142432.|School of Biosciences, Cardiff University, Museum Avenue, Cardiff, CF10 3AX, UK.
authors.forename: Vladimir L
authors.initials: VL
authors.lastname: Buchman
authors.affiliation: Institute of Physiologically Active Compounds, Russian Academy of Sciences, 1 Severniy proezd, Chernogolovka, Moscow Region, Russian Federation, 142432. ninkinan@cf.ac.uk.|School of Biosciences, Cardiff University, Museum Avenue, Cardiff, CF10 3AX, UK. ninkinan@cf.ac.uk.
authors.forename: Natalia
authors.identifier: 0000-0001-8570-5648
authors.initials: N
authors.lastname: Ninkina
mesh_terms: D000818:Animals; D015870:Gene Expression / Q000235:genetics; D017930:Genes, Reporter / Q000235:genetics*; D019426:Integrases / Q000235:genetics*; D007763:Lac Operon / Q000235:genetics; D051379:Mice; D018345:Mice, Knockout; D008822:Mice, Transgenic / Q000235:genetics; D022661:RNA, Untranslated / Q000235:genetics; D011995:Recombination, Genetic; D051844:alpha-Synuclein / Q000235:genetics*
chemical_list: C110853:Gt(ROSA)26Sor non-coding RNA, mouse; D022661:RNA, Untranslated; C497605:Snca protein, mouse; D051844:alpha-Synuclein; C045073:Cre recombinase; D019426:Integrases
keywords: Conditional gene knockout; LoxP/Cre recombination; Meiotic recombination; Synuclein; Transgenic mice