title: Effects of temperature, pH and sugar binding on the structures of lectins ebulin f and SELfd.
abstract: Ebulin f and SELfd are two lectins of Sambucus ebulus L. that show different stability and digestibility properties in gastric fluid due to their structural differences which may explain their different toxicological profiles. The main aim was to determine the effects of pH, temperature and sugar binding on the intrinsic structures of both proteins by fluorescence analyses. Quenching experiments were conducted, under different pH and temperature conditions, with acrylamide (uncharged) and iodide (charged), to study the possible changes of their intrinsic fluorescence. Results revealed that the native structure of SELfd is more folded than that of ebulin f. At pH 2.0, ebulin f displayed a more open structure than at neutral pH. It can be concluded that this is the main reason why ebulin f is accessible to pepsin action and more sensitive to degradation, in contrast to SELfd as we reported previously.
authors.affiliation: Nutrición y Bromatología, Facultad de Medicina, Universidad de Valladolid, E-47005 Valladolid, Spain.
authors.forename: Celia
authors.initials: C
authors.lastname: Carrillo
authors.affiliation: Farmacia y Tecnología Farmacéutica and IUFI (Instituto Universitario de Farmacia Industrial), Facultad de Farmacia, Universidad Complutense de Madrid, E-28040 Madrid, Spain.
authors.forename: Damián
authors.initials: D
authors.lastname: Cordoba-Diaz
authors.affiliation: Farmacia y Tecnología Farmacéutica and IUFI (Instituto Universitario de Farmacia Industrial), Facultad de Farmacia, Universidad Complutense de Madrid, E-28040 Madrid, Spain.
authors.forename: Manuel
authors.initials: M
authors.lastname: Cordoba-Diaz
authors.affiliation: Nutrición y Bromatología, Facultad de Medicina, Universidad de Valladolid, E-47005 Valladolid, Spain. Electronic address: girbes@bio.uva.es.
authors.forename: Tomás
authors.initials: T
authors.lastname: Girbés
authors.affiliation: Nutrición y Bromatología, Facultad de Medicina, Universidad de Valladolid, E-47005 Valladolid, Spain.
authors.forename: Pilar
authors.initials: P
authors.lastname: Jiménez
mesh_terms: D006863:Hydrogen-Ion Concentration; D037102:Lectins / Q000737:chemistry*; D010434:Pepsin A / Q000378:metabolism; D011487:Protein Conformation; D054790:Ribosome Inactivating Proteins, Type 2 / Q000737:chemistry*; D027923:Sambucus / Q000737:chemistry*; D013696:Temperature
chemical_list: D037102:Lectins; D054790:Ribosome Inactivating Proteins, Type 2; D010434:Pepsin A
keywords: 8-Anilino-1-naphthalenesulfonic acid (PubChem CID: 24890494); Acrylamide (PubChem CID: 6579); Dwarf elder; Ebulin f; Guanidinium chloride (PubChem CID: 5742); Hydrochloric acid (PubChem CID: 313); Lactose (PubChem CID: 84571); Lectin; Pepsin, lactose; SELfd; Sambucus ebulus; Sepharose (PubChem CID: 11966311); d-Galactose; d-Galactose (PubChem CID: 6036)