title: Non-invasive Quantitative Analysis of Specific Fat Accumulation in Subcutaneous Adipose Tissues using Raman Spectroscopy.
abstract: Subcutaneous adipose tissue (SAT), visceral adipose tissue (VAT), and fat beneath the dermis layer were investigated using a ball lens top hollow optical fiber Raman probe (BHRP). Hamsters were fed with trilinolein (TL) and tricaprin (TC) for six weeks and measurements were carried out every two weeks. The BHRP with an 800 μm diameter fused-silica ball lens was able to obtain information on the subcutaneous fat in a totally non-invasive manner. Changes in the concentration of TL and TC during the treatment were analyzed, and the relationship between fat accumulation and dietary fat was studied. It was found that SAT had, in general, a higher degree of unsaturation than VAT. The accumulation rate of TC found in SAT and VAT was 0.52 ± 0.38 and 0.58 ± 0.4%, respectively, while the TL accumulation rate was 4.45 ± 1.6 and 4.37 ± 2.4%, respectively. The results suggest different metabolic pathways for TC, a typical medium-chain fatty acid, and TL, a long-chain unsaturated fatty acid. Raman subsurface spectra were successfully obtained and used to analyze the subcutaneous fat layer. The accumulation rates of TL and TC found in skin fat were 5.01 ± 3.53% and 0.45 ± 0.36%, respectively. The results demonstrate the high feasibility of Raman spectroscopy for non-invasive analysis of adipose tissue.
authors.affiliation: Department of Biomedical Chemistry, School of Science and Technology, Kwansei Gakuin University 2-1, Gakuen, Sanda, Hyogo, 669-1337 Japan.
authors.forename: Phiranuphon
authors.initials: P
authors.lastname: Meksiarun
authors.affiliation: Department of Biomedical Chemistry, School of Science and Technology, Kwansei Gakuin University 2-1, Gakuen, Sanda, Hyogo, 669-1337 Japan.
authors.forename: Bibin B
authors.initials: BB
authors.lastname: Andriana
authors.affiliation: Department of Biomedical Chemistry, School of Science and Technology, Kwansei Gakuin University 2-1, Gakuen, Sanda, Hyogo, 669-1337 Japan.
authors.forename: Hiroko
authors.initials: H
authors.lastname: Matsuyoshi
authors.affiliation: Department of Biomedical Chemistry, School of Science and Technology, Kwansei Gakuin University 2-1, Gakuen, Sanda, Hyogo, 669-1337 Japan.
authors.forename: Hidetoshi
authors.initials: H
authors.lastname: Sato
mesh_terms: D000818:Animals; D008647:Mesocricetus; D013059:Spectrum Analysis, Raman / Q000379:methods*; D050151:Subcutaneous Fat / Q000166:cytology / Q000378:metabolism*; D014280:Triglycerides / Q000494:pharmacology
chemical_list: D014280:Triglycerides; C009564:trilinolein