title: Antibacterial activity and cytocompatibility of chitooligosaccharide-modified polyurethane membrane via polydopamine adhesive layer.
abstract: The aim of this study was to provide a convenient surface modification method for polyurethane (PU) membrane and evaluate its influence on hydrophilicity, antibacterial activity and cell functions, which are the most important factors for wound dressings. For this purpose, chitooligosaccharide (COS) was modified onto the surface of PU membrane based on the self-polymerization of dopamine (DOPA). Surface composition, morphology, hydrophilicity and surface energy of the original and modified PU membranes were characterized. Surface roughness and hydrophilicity of the PU membrane were obviously increased by modified with polydopamine (PDOPA) and COS. Antibacterial experiment against Escherichia coli and Staphylococcus aureus indicated that antibacterial activity of PU membrane increased only slightly by modified with PDOPA, but increased significantly by further modified with COS. Cells culture results revealed that COS-functionalized PU membrane is more beneficial to the adhesion and proliferation of NIH-3T3 cells compared to the original and PDOPA-modified PU membranes.
authors.affiliation: Department of Material Science and Engineering, Jinan University, Guangzhou 510632, PR China; Engineering Research Center of Artificial Organs and Materials, Ministry of Education, Guangzhou 510632, PR China.
authors.forename: Chuang
authors.initials: C
authors.lastname: Luo
authors.affiliation: Department of Material Science and Engineering, Jinan University, Guangzhou 510632, PR China; Engineering Research Center of Artificial Organs and Materials, Ministry of Education, Guangzhou 510632, PR China.
authors.forename: Wenjun
authors.initials: W
authors.lastname: Liu
authors.affiliation: Department of Material Science and Engineering, Jinan University, Guangzhou 510632, PR China. Electronic address: tluobh@jnu.edu.cn.
authors.forename: Binghong
authors.initials: B
authors.lastname: Luo
authors.affiliation: Department of Material Science and Engineering, Jinan University, Guangzhou 510632, PR China; Engineering Research Center of Artificial Organs and Materials, Ministry of Education, Guangzhou 510632, PR China.
authors.forename: Jinhuan
authors.initials: J
authors.lastname: Tian
authors.affiliation: Department of Material Science and Engineering, Jinan University, Guangzhou 510632, PR China; Engineering Research Center of Artificial Organs and Materials, Ministry of Education, Guangzhou 510632, PR China.
authors.forename: Wei
authors.initials: W
authors.lastname: Wen
authors.affiliation: Department of Material Science and Engineering, Jinan University, Guangzhou 510632, PR China; Engineering Research Center of Artificial Organs and Materials, Ministry of Education, Guangzhou 510632, PR China.
authors.forename: Mingxian
authors.initials: M
authors.lastname: Liu
authors.affiliation: Engineering Research Center of Artificial Organs and Materials, Ministry of Education, Guangzhou 510632, PR China. Electronic address: tcrz9@jnu.edu.cn.
authors.forename: Changren
authors.initials: C
authors.lastname: Zhou
mesh_terms: D000269:Adhesives; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001419:Bacteria / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002686:Chitin / Q000031:analogs & derivatives / Q000737:chemistry; D048271:Chitosan; D007211:Indoles / Q000494:pharmacology* / Q000633:toxicity; D051379:Mice; D041681:NIH 3T3 Cells; D009844:Oligosaccharides; D011108:Polymers / Q000494:pharmacology* / Q000633:toxicity; D011140:Polyurethanes / Q000494:pharmacology* / Q000633:toxicity; D013499:Surface Properties
chemical_list: D000269:Adhesives; D000900:Anti-Bacterial Agents; D007211:Indoles; D009844:Oligosaccharides; D011108:Polymers; D011140:Polyurethanes; C493484:oligochitosan; C568283:polydopamine; D002686:Chitin; D048271:Chitosan
keywords: Antibacterial properties; Chitooligosaccharide; Cytocompatibility; Dopamine; Polyurethane