title: Development of a novel polystyrene/metal-organic framework-199 electrospun nanofiber adsorbent for thin film microextraction of aldehydes in human urine.
abstract: In this work, electrospun polystyrene/metal-organic frameworks-199 (PS/MOF-199) nanofiber film was synthesized and investigated as a novel adsorbent for thin film microextraction (TFME) of aldehydes in human urine. Some properties of the prepared PS/MOF-199 nanofiber film, including morphology, structure, wettability, solvent stability and extraction performance were studied systematically. Porous fibrous structure, large surface area, good stability, strong hydrophobicity and excellent extraction efficiency were obtained for the film. Based on the PS/MOF-199 film, a thin film microextraction-high performance liquid chromatography (TFME-HPLC) method was developed, and the experimental parameters that affected the extraction and desorption were optimized. Under the optimal conditions, the limits of detection (LODs) were in the range of 4.2-17.3nmolL-1for the analysis of six aldehydes. Good linearity was achieved with correlation coefficients (R2) being lager than 0.9943. Satisfactory recovery (82-112%) and acceptable reproducibility (relative standard deviation: 2.1-13.3%) were also obtained for the method. The developed TFME-HPLC method has been successfully applied to the analysis of aldehyde metabolites in the urine samples of lung cancer patients and healthy people. The method possesses the advantages of simplicity, rapidity, cost-effective, sensitivity and non-invasion, it provides an alternative tool for the determination of aldehydes in complex sample matrices.
authors.affiliation: Key Laboratory of Pesticide & Chemical Biology, Ministry of Education, College of Chemistry, Central China Normal University, Wuhan 430079, China.
authors.forename: Feilong
authors.initials: F
authors.lastname: Liu
authors.affiliation: Key Laboratory of Pesticide & Chemical Biology, Ministry of Education, College of Chemistry, Central China Normal University, Wuhan 430079, China. Electronic address: huixu@mail.ccnu.edu.cn.
authors.forename: Hui
authors.initials: H
authors.lastname: Xu
mesh_terms: D000447:Aldehydes / Q000302:isolation & purification / Q000652:urine*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D055664:Electrochemical Techniques; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D000073396:Metal-Organic Frameworks / Q000737:chemistry*; D008855:Microscopy, Electron, Scanning; D057139:Nanofibers / Q000737:chemistry* / Q000648:ultrastructure; D011137:Polystyrenes / Q000737:chemistry*; D015203:Reproducibility of Results; D052617:Solid Phase Microextraction / Q000379:methods*; D014961:X-Ray Diffraction
chemical_list: D000447:Aldehydes; D000073396:Metal-Organic Frameworks; D011137:Polystyrenes
keywords: Aldehydes; Electrospinning; High performance liquid chromatography; Nonafiber; Polystyrene/metal-organic frameworks-199; Thin film microextraction