title: Associations of Caspase-3 gene polymorphism with lumbar disc herniation.
abstract: To investigate Caspase-3 gene polymorphisms (rs4647693 G/A, rs4647610 A/G, and rs12108497 T/C) and susceptibility to lumbar intervertebral disc herniation (LDH). The genotype frequency distributions of the polymorphisms were detected by polymerase chain reaction-restriction fragment length polymorphism in 107 LDH patients (case group) and 121 healthy individuals (control group). SHEsis software was used to conduct gene linkage disequilibrium and haplotype analysis. Regression analysis was used to analyze possible risk factors for LDH. Statistically significant differences in family history of LDH, amateur sports, leisure activities, bed types, and spine load grade were found between the case and control groups. The distribution of allele and genotype frequencies of rs4647693 G/A, rs4647610 A/G, and rs12108497 T/C polymorphisms of Caspase-3 were significantly different between the case and control groups. Haplotype analysis showed that the G-G-C (rs4647693-rs4647610-rs12108497) haplotype might be a risk factor for LDH, whereas the A-A-T haplotype might be a protective factor (p < 0.05). Binary logistic regression analysis showed that the GA+AA genotype of rs4647693 was negatively associated with the risk of LDH, whereas high spine load grade was positively associated with the risk of LDH. These findings revealed that rs4647693 G/A, rs4647610 A/G, and rs12108497 T/C polymorphisms of Caspase-3 may be associated with susceptibility to LDH and that interaction and modification effects may exist between Caspase-3 polymorphisms.
authors.affiliation: Department of Anesthesiology, Changsha Central Hospital, Changsha, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Fang
authors.affiliation: Department of Oncology, Mawangdui District of Hunan Provincial People's Hospital, Changsha, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Qiu
authors.affiliation: Department of Pain Medicine, First Affiliated Hospital of Guangxi Medical University, Nanning, China. Electronic address: jiangzongbin2016@sina.com.
authors.forename: Zong-Bin
authors.initials: ZB
authors.lastname: Jiang
authors.affiliation: Department of Pain Medicine, First Affiliated Hospital of Guangxi Medical University, Nanning, China.
authors.forename: Sheng-Rong
authors.initials: SR
authors.lastname: Xu
authors.affiliation: Department of Pain Medicine, First Affiliated Hospital of Guangxi Medical University, Nanning, China.
authors.forename: Zeng-Hua
authors.initials: ZH
authors.lastname: Zhou
authors.affiliation: Department of Pain Medicine, First Affiliated Hospital of Guangxi Medical University, Nanning, China.
authors.forename: Rui-Lin
authors.initials: RL
authors.lastname: He
mesh_terms: D000328:Adult; D000483:Alleles; D016022:Case-Control Studies; D053148:Caspase 3 / Q000235:genetics*; D005260:Female; D005787:Gene Frequency; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease; D006239:Haplotypes / Q000235:genetics; D006801:Humans; D007405:Intervertebral Disc Displacement / Q000201:enzymology* / Q000235:genetics*; D015810:Linkage Disequilibrium / Q000235:genetics; D016015:Logistic Models; D008159:Lumbar Vertebrae / Q000473:pathology*; D008297:Male; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*
chemical_list: D053148:Caspase 3
keywords: Caspase-3; Haplotypes analysis; Lumbar intervertebral disc herniation; Polymorphism; Susceptibility