title: Development of an indirect immunofluorescence based assay for diagnosis of ulcerative colitis in Indian population.
abstract: The prevalence of Ulcerative Colitis (UC), once thought to be negligible, has increases exponentially in the Indian population. The development of novel, cost effective and time efficient Indirect Immunofluorescence (IIF) based assay for detection of anti-neutrophil cytoplasmic antibodies (ANCA) and diagnosis of UC in the Indian population is discussed. A novel IIF based assay was developed using intact nuclei from human neutrophils to detect atypical p-ANCA in patients suffering from UC. Sera from 45 patients diagnosed with UC, 45 healthy controls and one related disease control were tested using a novel UC-ANCA assay and validated by commercially available ANCA IIF assay. Prevalence of ANCA amongst UC patients in the Indian population was determined. Atypical p-ANCA was detected in 86.6% of the patients using the UC-ANCA assay as compared to 71.1% using the commercial ANCA assay. The validation of UC-ANCA assay with a commercially available ANCA IIF assay resulted in higher sensitivity. The UC-ANCA assay proved to be not only enhanced in terms of performance but also comparatively economical and rapid. The novel UC-ANCA assay may prove to be very useful in identification and differentiation of UC patients from typical ANCA positive subjects suffering from other autoimmune diseases at one tenth the cost of clinically available ANCA IIF tests which will immensely benefit the cost constrained diagnostic field of developing countries.
authors.affiliation: Laboratory of Applied Immunology, Research and Development Division, Yashraj Biotechnology Ltd., Navi Mumbai 400705, India; Department of Medical Biotechnology, MGM Institute of Health Sciences, Kamothe, Navi Mumbai 410209, India.
authors.forename: Allan V
authors.initials: AV
authors.lastname: Rodrigues
authors.affiliation: Department of Medical Biotechnology, MGM Institute of Health Sciences, Kamothe, Navi Mumbai 410209, India.
authors.forename: Chander P
authors.initials: CP
authors.lastname: Puri
authors.affiliation: Laboratory of Applied Immunology, Research and Development Division, Yashraj Biotechnology Ltd., Navi Mumbai 400705, India; Department of Medical Biotechnology, MGM Institute of Health Sciences, Kamothe, Navi Mumbai 410209, India.
authors.forename: Paresh B
authors.initials: PB
authors.lastname: Bhanushali
authors.affiliation: Laboratory of Applied Immunology, Research and Development Division, Yashraj Biotechnology Ltd., Navi Mumbai 400705, India.
authors.forename: Kunal K
authors.initials: KK
authors.lastname: Shukla
authors.affiliation: Laboratory of Applied Immunology, Research and Development Division, Yashraj Biotechnology Ltd., Navi Mumbai 400705, India.
authors.forename: Sampurna
authors.initials: S
authors.lastname: Roychoudhury
authors.affiliation: Laboratory of Applied Immunology, Research and Development Division, Yashraj Biotechnology Ltd., Navi Mumbai 400705, India; Department of Biochemistry, National Institute for Research in Reproductive Health, Jehangir Merwanji Street, Parel, Mumbai 400012 India. Electronic address: sham83badgujar@gmail.com.
authors.forename: Shamkant B
authors.initials: SB
authors.lastname: Badgujar
mesh_terms: D019268:Antibodies, Antineutrophil Cytoplasmic; D015415:Biomarkers; D016022:Case-Control Studies; D003093:Colitis, Ulcerative / Q000175:diagnosis*; D019084:Fluorescent Antibody Technique, Indirect / Q000379:methods / Q000592:standards; D006801:Humans; D007194:India; D015212:Inflammatory Bowel Diseases / Q000175:diagnosis
chemical_list: D019268:Antibodies, Antineutrophil Cytoplasmic; D015415:Biomarkers
keywords: Anti neutrophil cytoplasmic antibodies; Indirect immunofluorescence; Inflammatory bowel disease; Ulcerative colitis