title: Immunofluorescence of Histone Proteins.
abstract: The identification of a vast array of posttranslational modifications of histone proteins during cell cycle, repair, replication, and transcription has created the challenge of determining structure-function relationships for individual modifications and combinations of modifications. Some of this information can be gathered from indirect immunofluorescence, where the location and cell cycle relationships can be readily identified. Here we present an immunofluorescence protocol that is adapted for the use in histone modifications.
authors.affiliation: Departments of Oncology and Cell Biology, Faculty of Medicine and Dentistry, University of Alberta, 11560 University Avenue, Edmonton, AB, Canada, T6 G 1Z2.
authors.forename: Hilmar
authors.initials: H
authors.lastname: Strickfaden
authors.affiliation: Departments of Oncology and Cell Biology, Faculty of Medicine and Dentistry, University of Alberta, 11560 University Avenue, Edmonton, AB, Canada, T6 G 1Z2. mhendzel@ualberta.ca.
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Hendzel
mesh_terms: D002453:Cell Cycle / Q000235:genetics / Q000502:physiology; D004261:DNA Replication / Q000235:genetics / Q000502:physiology; D005455:Fluorescent Antibody Technique / Q000379:methods*; D042421:Histone Code; D006657:Histones / Q000378:metabolism*; D006801:Humans; D011499:Protein Processing, Post-Translational
chemical_list: D006657:Histones
keywords: Histone modifications; Immunofluorescence