title: Featured Article: Transcriptional landscape analysis identifies differently expressed genes involved in follicle-stimulating hormone induced postmenopausal osteoporosis.
abstract: Osteoporosis is a disorder associated with bone tissue reorganization, bone mass, and mineral density. Osteoporosis can severely affect postmenopausal women, causing bone fragility and osteoporotic fractures. The aim of the current study was to compare blood mRNA profiles of postmenopausal women with and without osteoporosis, with the aim of finding different gene expressions and thus targets for future osteoporosis biomarker studies. Our study consisted of transcriptome analysis of whole blood serum from 12 elderly female osteoporotic patients and 12 non-osteoporotic elderly female controls. The transcriptome analysis was performed with RNA sequencing technology. For data analysis, the edgeR package of R Bioconductor was used. Two hundred and fourteen genes were expressed differently in osteoporotic compared with non-osteoporotic patients. Statistical analysis revealed 20 differently expressed genes with a false discovery rate of less than 1.47 × 10-4among osteoporotic patients. The expression of 10 genes were up-regulated and 10 down-regulated. Further statistical analysis identified a potential osteoporosis mRNA biomarker pattern consisting of six genes: CACNA1G, ALG13, SBK1, GGT7, MBNL3, and RIOK3. Functional ingenuity pathway analysis identified the strongest candidate genes with regard to potential involvement in a follicle-stimulating hormone activated network of increased osteoclast activity and hypogonadal bone loss. The differentially expressed genes identified in this study may contribute to future research of postmenopausal osteoporosis blood biomarkers.
authors.affiliation: 1 Department of Traumatology and Orthopedics, University of Tartu, Tartu 51014, Estonia.|2 Clinic of Traumatology and Orthopedics, Tartu University Hospital, Tartu 51014, Estonia.
authors.forename: Katre
authors.initials: K
authors.lastname: Maasalu
authors.affiliation: 1 Department of Traumatology and Orthopedics, University of Tartu, Tartu 51014, Estonia.
authors.forename: Ott
authors.initials: O
authors.lastname: Laius
authors.affiliation: 1 Department of Traumatology and Orthopedics, University of Tartu, Tartu 51014, Estonia.
authors.forename: Lidiia
authors.initials: L
authors.lastname: Zhytnik
authors.affiliation: 3 Department of Pathophysiology, University of Tartu, Tartu 50411, Estonia.
authors.forename: Sulev
authors.initials: S
authors.lastname: Kõks
authors.affiliation: 3 Department of Pathophysiology, University of Tartu, Tartu 50411, Estonia.
authors.forename: Ele
authors.initials: E
authors.lastname: Prans
authors.affiliation: 3 Department of Pathophysiology, University of Tartu, Tartu 50411, Estonia.
authors.forename: Ene
authors.initials: E
authors.lastname: Reimann
authors.affiliation: 1 Department of Traumatology and Orthopedics, University of Tartu, Tartu 51014, Estonia.|2 Clinic of Traumatology and Orthopedics, Tartu University Hospital, Tartu 51014, Estonia.
authors.forename: Aare
authors.initials: A
authors.lastname: Märtson
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D001483:Base Sequence; D015519:Bone Density / Q000235:genetics* / Q000502:physiology; D020747:Calcium Channels, T-Type / Q000235:genetics; D005260:Female; D005640:Follicle Stimulating Hormone / Q000235:genetics / Q000378:metabolism*; D020869:Gene Expression Profiling; D005819:Genetic Markers / Q000235:genetics*; D006801:Humans; D008875:Middle Aged; D017351:N-Acetylglucosaminyltransferases / Q000235:genetics; D015663:Osteoporosis, Postmenopausal / Q000175:diagnosis / Q000235:genetics*; D011494:Protein Kinases / Q000235:genetics; D017346:Protein Serine-Threonine Kinases / Q000235:genetics; D012333:RNA, Messenger / Q000097:blood* / Q000235:genetics; D016601:RNA-Binding Proteins / Q000235:genetics; D017423:Sequence Analysis, RNA; D014158:Transcription, Genetic
chemical_list: C121855:CACNA1G protein, human; D020747:Calcium Channels, T-Type; D005819:Genetic Markers; C467324:MBNL3 protein, human; D012333:RNA, Messenger; D016601:RNA-Binding Proteins; D005640:Follicle Stimulating Hormone; C583889:ALG13 protein, human; D017351:N-Acetylglucosaminyltransferases; D011494:Protein Kinases; C561879:SH3-binding domain kinase 1, human; D017346:Protein Serine-Threonine Kinases; C534426:RIO kinase 3, human
keywords: Bone; age; biomarkers; female; musculoskeletal; transcriptome