title: Revisiting the expression and function of follicle-stimulation hormone receptor in human umbilical vein endothelial cells.
abstract: Expression of follicle-stimulation hormone receptor (FSHR) is confined to gonads and at low levels to some extragonadal tissues like human umbilical vein endothelial cells (HUVEC). FSH-FSHR signaling was shown to promote HUVEC angiogenesis and thereafter suggested to have an influential role in pregnancy. We revisited hereby the expression and functionality of FSHR in HUVECs angiogenesis, and were unable to reproduce the FSHR expression in human umbilical cord, HUVECs or immortalized HUVECs (HUV-ST). Positive controls as granulosa cells and HEK293 cells stably transfected with human FSHR cDNA expressed FSHR signal. In contrast to positive control VEGF, FSH treatment showed no effects on tube formation, nitric oxide production, wound healing or cell proliferation in HUVEC/HUV-ST. Thus, it remains open whether the FSH-FSHR activation has a direct regulatory role in the angiogenesis of HUVECs.
authors.affiliation: Department of Reproduction and Gynecological Endocrinology, Medical University of Bialystok, 15276 Bialystok, Poland.
authors.forename: Joanna
authors.initials: J
authors.lastname: Stelmaszewska
authors.affiliation: Institute of Biomedicine, Department of Physiology, University of Turku, 20520 Turku, Finland.|Department of Biology and Pathology of Human Reproduction, Institute of Animal Reproduction and Food Research, Polish Academy of Science, 10714 Olsztyn, Poland.
authors.forename: Marcin
authors.initials: M
authors.lastname: Chrusciel
authors.affiliation: Institute of Biomedicine, Department of Physiology, University of Turku, 20520 Turku, Finland.
authors.forename: Milena
authors.initials: M
authors.lastname: Doroszko
authors.affiliation: Institute of Biomedicine, Department of Cell Biology and Anatomy, University of Turku, 20520 Turku, Finland.
authors.forename: Malin
authors.initials: M
authors.lastname: Akerfelt
authors.affiliation: Department of Biology and Pathology of Human Reproduction, Institute of Animal Reproduction and Food Research, Polish Academy of Science, 10714 Olsztyn, Poland.
authors.forename: Donata
authors.initials: D
authors.lastname: Ponikwicka-Tyszko
authors.affiliation: Institute of Biomedicine, Department of Cell Biology and Anatomy, University of Turku, 20520 Turku, Finland.
authors.forename: Matthias
authors.initials: M
authors.lastname: Nees
authors.affiliation: Regenerative Immunology and Aging, Berlin-Brandenburg Center for Regenerative Therapies, CVK Charité University Medicine, 13353 Berlin, Germany.
authors.forename: Marco
authors.initials: M
authors.lastname: Frentsch
authors.affiliation: State Key Lab for Agrobiotechnology, China Agriculture University, 100193 Beijing, China.
authors.forename: Xiangdong
authors.initials: X
authors.lastname: Li
authors.affiliation: Institute of Biomedicine, Department of Physiology, University of Turku, 20520 Turku, Finland.
authors.forename: Jukka
authors.initials: J
authors.lastname: Kero
authors.affiliation: Institute of Biomedicine, Department of Physiology, University of Turku, 20520 Turku, Finland.|Institute of Reproductive and Developmental Biology, Imperial College London, W12 ONN London, UK.
authors.forename: Ilpo
authors.initials: I
authors.lastname: Huhtaniemi
authors.affiliation: Department of Reproduction and Gynecological Endocrinology, Medical University of Bialystok, 15276 Bialystok, Poland.|Department of Biology and Pathology of Human Reproduction, Institute of Animal Reproduction and Food Research, Polish Academy of Science, 10714 Olsztyn, Poland.
authors.forename: Slawomir
authors.initials: S
authors.lastname: Wolczynski
authors.affiliation: Department of Reproduction and Gynecological Endocrinology, Medical University of Bialystok, 15276 Bialystok, Poland.|Institute of Biomedicine, Department of Physiology, University of Turku, 20520 Turku, Finland.
authors.forename: Nafis A
authors.initials: NA
authors.lastname: Rahman
mesh_terms: D049109:Cell Proliferation / Q000187:drug effects*; D005260:Female; D005640:Follicle Stimulating Hormone / Q000494:pharmacology*; D005786:Gene Expression Regulation / Q000187:drug effects*; D057809:HEK293 Cells; D061307:Human Umbilical Vein Endothelial Cells / Q000166:cytology / Q000378:metabolism*; D006801:Humans; D018919:Neovascularization, Physiologic / Q000187:drug effects*; D011247:Pregnancy; D011962:Receptors, FSH / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D011962:Receptors, FSH; D005640:Follicle Stimulating Hormone