title: Synthesis, characterization and radiolabeling of folic acid modified nanostructured lipid carriers as a contrast agent and drug delivery system.
abstract: Nanostructured lipid carriers (NLCs) are the new generation of solid lipid drug delivery systems. Their suitability as contrast agents for gamma scintigraphy is an attracting major attention. The aim of current study was to prepare surface modified nanostructured lipid carrier system for paclitaxel (PTX) with active targeting and imaging functions. In accordance with the purpose of study, PTX loaded nanostructured lipid carriers (NLCs) prepared, modified with a folate derivative and radiolabeled with technetium-99m tricarbonyl complex (99mTc(CO)3+). Cellular incorporation ratios of radiolabeled nanoparticles (99mTc(CO)3-PTX-NLC) were investigated in vitro on three cancer cell lines. Additionally in vivo animal studies conducted to evaluate biological behavior of99mTc(CO)3-PTX-NLC on female Wistar Albino rats. Biodistribution results showed that the folate derivative modified99mTc(CO)3-PTX-NLC had considerably higher uptake in folate receptor positive organs. The data obtained from present study could be useful in the design of biodegradable drug carriers of PTX and folate receptor based tumor imaging agents.
authors.affiliation: Department of Nuclear Applications, Institute of Nuclear Sciences Ege University, Bornova, 35100 İzmir, Turkey.
authors.forename: Eser
authors.initials: E
authors.lastname: Ucar
authors.affiliation: Department of Nuclear Applications, Institute of Nuclear Sciences Ege University, Bornova, 35100 İzmir, Turkey. Electronic address: serap.teksoz@ege.edu.tr.
authors.forename: Serap
authors.initials: S
authors.lastname: Teksoz
authors.affiliation: Department of Nuclear Applications, Institute of Nuclear Sciences Ege University, Bornova, 35100 İzmir, Turkey.
authors.forename: Cigdem
authors.initials: C
authors.lastname: Ichedef
authors.affiliation: Department of Nuclear Applications, Institute of Nuclear Sciences Ege University, Bornova, 35100 İzmir, Turkey.
authors.forename: Ayfer Yurt
authors.initials: AY
authors.lastname: Kilcar
authors.affiliation: Department of Nuclear Applications, Institute of Nuclear Sciences Ege University, Bornova, 35100 İzmir, Turkey.
authors.forename: E Ilker
authors.initials: EI
authors.lastname: Medine
authors.affiliation: Department of Nuclear Applications, Institute of Nuclear Sciences Ege University, Bornova, 35100 İzmir, Turkey.
authors.forename: Kadir
authors.initials: K
authors.lastname: Ari
authors.affiliation: Department of Nuclear Medicine, School of Medicine, Celal Bayar University, Manisa, Turkey.
authors.forename: Yasemin
authors.initials: Y
authors.lastname: Parlak
authors.affiliation: Department of Nuclear Medicine, School of Medicine, Celal Bayar University, Manisa, Turkey.
authors.forename: B Elvan
authors.initials: BE
authors.lastname: Sayit Bilgin
authors.affiliation: Department of Nuclear Applications, Institute of Nuclear Sciences Ege University, Bornova, 35100 İzmir, Turkey.
authors.forename: Perihan
authors.initials: P
authors.lastname: Unak
mesh_terms: D000072283:A549 Cells; D000818:Animals; D003287:Contrast Media / Q000008:administration & dosage* / Q000138:chemical synthesis / Q000737:chemistry; D004337:Drug Carriers / Q000008:administration & dosage / Q000138:chemical synthesis / Q000737:chemistry; D016503:Drug Delivery Systems; D005260:Female; D005492:Folic Acid / Q000737:chemistry; D006367:HeLa Cells; D006801:Humans; D008055:Lipids / Q000008:administration & dosage / Q000138:chemical synthesis / Q000737:chemistry; D061986:MCF-7 Cells; D008856:Microscopy, Fluorescence; D049329:Nanostructures / Q000008:administration & dosage / Q000737:chemistry; D017239:Paclitaxel / Q000008:administration & dosage; D051381:Rats; D017208:Rats, Wistar; D013667:Technetium / Q000008:administration & dosage* / Q000737:chemistry; D014018:Tissue Distribution
chemical_list: D003287:Contrast Media; D004337:Drug Carriers; D008055:Lipids; D013667:Technetium; D005492:Folic Acid; D017239:Paclitaxel
keywords: Biodistribution; Cell culture; Nanostructured lipid carriers; Paclitaxel; Radiolabeling; Technetium-99m