title: A Simple Thermoplastic Substrate Containing Hierarchical Silica Lamellae for High-Molecular-Weight DNA Extraction.
abstract: An inexpensive, magnetic thermoplastic nanomaterial is developed utilizing a hierarchical layering of micro- and nanoscale silica lamellae to create a high-surface-area and low-shear substrate capable of capturing vast amounts of ultrahigh-molecular-weight DNA. Extraction is performed via a simple 45 min process and is capable of achieving binding capacities up to 1 000 000 times greater than silica microparticles.
authors.affiliation: Biomedical Engineering Department, Johns Hopkins University, Baltimore, MD 21218, USA.
authors.forename: Ye
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Biomedical Engineering Department, Johns Hopkins University, Baltimore, MD 21218, USA.|Institute of Bioengineering and Nanotechnology, Agency of Science Technology and Research, Singapore, 138669, Singapore.
authors.forename: Yi
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Circulomics Inc, Baltimore, MD, 21211, USA.
authors.forename: Jeffrey M
authors.initials: JM
authors.lastname: Burke
authors.affiliation: Pacific Biosciences Inc, Menlo Park, CA 94025, 94025, USA.
authors.forename: Kristin
authors.initials: K
authors.lastname: Gleitsman
authors.affiliation: Biomedical Engineering Department, Johns Hopkins University, Baltimore, MD 21218, USA.
authors.forename: Sarah M
authors.initials: SM
authors.lastname: Friedrich
authors.affiliation: Circulomics Inc, Baltimore, MD, 21211, USA.
authors.forename: Kelvin J
authors.initials: KJ
authors.lastname: Liu
authors.affiliation: Biomedical Engineering Department, Johns Hopkins University, Baltimore, MD 21218, USA.|Mechanical Engineering Department, Johns Hopkins University, Baltimore, MD, 21218, USA.|Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University, Baltimore, MD, 21218, USA.|Center of Cancer Nanotechnology Excellence, Johns Hopkins University, Baltimore, MD, 21218, USA.
authors.forename: Tza-Huei
authors.initials: TH
authors.lastname: Wang
mesh_terms: D004247:DNA / Q000737:chemistry* / Q000302:isolation & purification*; D006801:Humans; D061986:MCF-7 Cells; D008280:Magnetics; D008970:Molecular Weight; D049329:Nanostructures / Q000737:chemistry*; D012822:Silicon Dioxide / Q000737:chemistry*; D052616:Solid Phase Extraction / Q000379:methods*
chemical_list: D012822:Silicon Dioxide; D004247:DNA
keywords: high molecular weight; long read sequencing; nanoscale silica; solid-phase extraction; thermoplastic