title: Enhanced colloidal stability, solubility and rapid dissolution of resveratrol by nanocomplexation with soy protein isolate.
abstract: The polyphenolic compound resveratrol has received significant attention due to its many pharmacological actions such as anti-cancer, anti-inflammatory, antioxidant and antimicrobial activities. However, poor solubility and stability are major impediments for resveratrol's clinical effectiveness. In this work we have encapsulated resveratrol into soy protein isolate nanoparticles using a simple rotary evaporation technique. Resveratrol-loaded nanoparticles were around 100nm in diameter and negatively charged. Nano-encapsulated resveratrol was found to be in amorphous form and showed more than two times higher solubility with significantly increased dissolution when compared to free resveratrol. Finally, an in-vitro NF-κB inhibition assay revealed that encapsulated resveratrol was stable and retained bioactivity. This new formulation of resveratrol has the potential to boost the clinical effectiveness of this drug and could be utilised for other poorly soluble hydrophobic drugs.
authors.affiliation: The School of Pharmacy, The University of Queensland, Brisbane, QLD 4072, Australia; Inflammatory Disease Biology and Therapeutics Group, Mater Research Institute - The University of Queensland, Translational Research Institute, 37 Kent St, Woolloongabba, QLD 4102, Australia.
authors.forename: Naisarg
authors.initials: N
authors.lastname: Pujara
authors.affiliation: The School of Pharmacy, The University of Queensland, Brisbane, QLD 4072, Australia; Inflammatory Disease Biology and Therapeutics Group, Mater Research Institute - The University of Queensland, Translational Research Institute, 37 Kent St, Woolloongabba, QLD 4102, Australia.
authors.forename: Siddharth
authors.initials: S
authors.lastname: Jambhrunkar
authors.affiliation: Inflammatory Disease Biology and Therapeutics Group, Mater Research Institute - The University of Queensland, Translational Research Institute, 37 Kent St, Woolloongabba, QLD 4102, Australia.
authors.forename: Kuan Yau
authors.initials: KY
authors.lastname: Wong
authors.affiliation: Inflammatory Disease Biology and Therapeutics Group, Mater Research Institute - The University of Queensland, Translational Research Institute, 37 Kent St, Woolloongabba, QLD 4102, Australia.
authors.forename: Michael
authors.initials: M
authors.lastname: McGuckin
authors.affiliation: The School of Pharmacy, The University of Queensland, Brisbane, QLD 4072, Australia; Inflammatory Disease Biology and Therapeutics Group, Mater Research Institute - The University of Queensland, Translational Research Institute, 37 Kent St, Woolloongabba, QLD 4102, Australia. Electronic address: a.popat@uq.edu.au.
authors.forename: Amirali
authors.initials: A
authors.lastname: Popat
mesh_terms: D000818:Animals; D002460:Cell Line; D003102:Colloids; D003692:Delayed-Action Preparations / Q000737:chemistry* / Q000378:metabolism; D004339:Drug Compounding / Q000379:methods; D065546:Drug Liberation; D004355:Drug Stability; D015870:Gene Expression; D017930:Genes, Reporter; D049452:Green Fluorescent Proteins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism; D057927:Hydrophobic and Hydrophilic Interactions; D008264:Macrophages / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D051379:Mice; D016328:NF-kappa B / Q000037:antagonists & inhibitors* / Q000235:genetics / Q000378:metabolism; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D010316:Particle Size; D000077185:Resveratrol; D012995:Solubility; D030262:Soybean Proteins / Q000737:chemistry*; D055672:Static Electricity; D013267:Stilbenes / Q000737:chemistry* / Q000494:pharmacology
chemical_list: D003102:Colloids; D003692:Delayed-Action Preparations; D016328:NF-kappa B; D030262:Soybean Proteins; D013267:Stilbenes; D049452:Green Fluorescent Proteins; D000077185:Resveratrol
keywords: Colloidal stability; Control release; Nanocomplex; Resveratrol; Solubility; Soy protein isolate