title: Protein-based inverse opals: A novel support for enzyme immobilization.
abstract: In this study, protein-based inverse opals were prepared for the first time by using the colloidal crystal templating method. The preparation process involved three steps including filling the templates with protein molecules, crosslinking, and template removal. The obtained inverse opals were used to immobilize Penicillin G acylase (PGA) because of its intrinsic biocompatible property. The immobilization process was optimized and the properties of the immobilized PGA (PGA@IO) were investigated. PGA@IO exhibited improved thermal and pH stability compared with its free counterpart. After reusing nine times, it retained 70% of the initial activity. Besides, the PGA@IO retained high activity during the hydrolysis reactions in continuous catalysis in packed-bed reactor (PBR) after 15 days.
authors.affiliation: School of Chemical Engineering and Technology, Hebei University of Technology, Guangrong Road, Hongqiao District, Tianjin 300130, PR China.
authors.forename: Yanjun
authors.initials: Y
authors.lastname: Jiang
authors.affiliation: School of Chemical Engineering and Technology, Hebei University of Technology, Guangrong Road, Hongqiao District, Tianjin 300130, PR China.
authors.forename: Wenya
authors.initials: W
authors.lastname: Sun
authors.affiliation: School of Chemical Engineering and Technology, Hebei University of Technology, Guangrong Road, Hongqiao District, Tianjin 300130, PR China.
authors.forename: Yaping
authors.initials: Y
authors.lastname: Wang
authors.affiliation: School of Chemical Engineering and Technology, Hebei University of Technology, Guangrong Road, Hongqiao District, Tianjin 300130, PR China.
authors.forename: Lihui
authors.initials: L
authors.lastname: Wang
authors.affiliation: School of Chemical Engineering and Technology, Hebei University of Technology, Guangrong Road, Hongqiao District, Tianjin 300130, PR China.
authors.forename: Liya
authors.initials: L
authors.lastname: Zhou
authors.affiliation: School of Chemical Engineering and Technology, Hebei University of Technology, Guangrong Road, Hongqiao District, Tianjin 300130, PR China; Hebei Provincial Key Laboratory of Green Chemical Technology and High Efficient Energy Saving, Hebei University of Technology, 8 Guangrong Road, Hongqiao District, Tianjin 300130, PR China. Electronic address: jgao@hebut.edu.cn.
authors.forename: Jing
authors.initials: J
authors.lastname: Gao
authors.affiliation: School of Chemical Engineering and Technology, Hebei University of Technology, Guangrong Road, Hongqiao District, Tianjin 300130, PR China.
authors.forename: Ying
authors.initials: Y
authors.lastname: He
authors.affiliation: School of Chemical Engineering and Technology, Hebei University of Technology, Guangrong Road, Hongqiao District, Tianjin 300130, PR China.
authors.forename: Li
authors.initials: L
authors.lastname: Ma
authors.affiliation: School of Chemical Engineering and Technology, Hebei University of Technology, Guangrong Road, Hongqiao District, Tianjin 300130, PR China. Electronic address: xuzhang@hebut.edu.cn.
authors.forename: Xu
authors.initials: X
authors.lastname: Zhang
mesh_terms: D000818:Animals; D055162:Biocatalysis; D001672:Biocompatible Materials / Q000737:chemistry; D019149:Bioreactors; D001709:Biotechnology; D002417:Cattle; D003102:Colloids; D003432:Cross-Linking Reagents; D003460:Crystallization; D004795:Enzyme Stability; D004800:Enzymes, Immobilized / Q000378:metabolism; D006868:Hydrolysis; D010399:Penicillin Amidase / Q000378:metabolism; D011506:Proteins / Q000737:chemistry*; D012710:Serum Albumin, Bovine / Q000737:chemistry
chemical_list: D001672:Biocompatible Materials; D003102:Colloids; D003432:Cross-Linking Reagents; D004800:Enzymes, Immobilized; D011506:Proteins; D012710:Serum Albumin, Bovine; D010399:Penicillin Amidase
keywords: Biocatalysis; Immobilized enzyme; Penicillin G acylase; Protein-based inverse opal