title: Molecular Characteristics of Mycobacterium tuberculosis Strains Isolated from Cutaneous Tuberculosis Patients in China.
abstract: Cutaneous tuberculosis (CTB) is probably underreported due to difficulties in detection and diagnosis. To address this issue, genotypes of Mycobacterium tuberculosis strains isolated from 30 patients with CTB were mapped at multiple loci, namely, RD105 deletions, spacer oligonucleotides, and Mycobacterial Interspersed Repetitive Unit-Variable Number Tandem Repeats (MIRU-VNTRs). Fifty-eight strains of pulmonary tuberculosis (PTB) were mapped as experimental controls. Drug resistance-associated gene mutations were determined by amplicon sequencing of target regions within 7 genes. Beijing family isolates were the most prevalent strains in CTB and PTB. MIRU-VNTR typing separated the Beijing strains from the non-Beijing strains, and the majority of CTB could be separated from PTB counterparts. Drug resistance determining regions showed only one CTB strain expressing isomazid resistance. Thus, while the CTB strains belonged to the same phylogenetic lineages and sub-lineages as the PTB strains, they differed at the level of several MIRU-VNTRs and in the proportion of drug resistance.
authors.affiliation: Institute of Dermatology, Chinese Academy of Medical Sciences and Peking Union Medical College, Nanjing, China.
authors.forename: Haiqin
authors.initials: H
authors.lastname: Jiang
authors.forename: Yali
authors.initials: Y
authors.lastname: Jin
authors.forename: Varalakshmi
authors.initials: V
authors.lastname: Vissa
authors.forename: Liangfen
authors.initials: L
authors.lastname: Zhang
authors.forename: Weijun
authors.initials: W
authors.lastname: Liu
authors.forename: Lianhua
authors.initials: L
authors.lastname: Qin
authors.forename: Kanglin
authors.initials: K
authors.lastname: Wan
authors.forename: Xiaocui
authors.initials: X
authors.lastname: Wu
authors.forename: Hongsheng
authors.initials: H
authors.lastname: Wang
authors.forename: Weida
authors.initials: W
authors.lastname: Liu
authors.forename: Baoxi
authors.initials: B
authors.lastname: Wang
mesh_terms: D000328:Adult; D000995:Antitubercular Agents / Q000627:therapeutic use; D016022:Case-Control Studies; D002681:China / Q000453:epidemiology; D004269:DNA, Bacterial / Q000235:genetics* / Q000302:isolation & purification; D024881:Drug Resistance, Bacterial / Q000235:genetics; D005260:Female; D005838:Genotype; D006801:Humans; D020071:Interspersed Repetitive Sequences; D008297:Male; D008826:Microbial Sensitivity Tests; D008875:Middle Aged; D018598:Minisatellite Repeats; D025202:Molecular Diagnostic Techniques; D009161:Mycobacterium / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification; D010641:Phenotype; D010802:Phylogeny; D012867:Skin / Q000382:microbiology*; D014382:Tuberculosis, Cutaneous / Q000175:diagnosis / Q000188:drug therapy / Q000453:epidemiology / Q000382:microbiology*
chemical_list: D000995:Antitubercular Agents; D004269:DNA, Bacterial