title: Transmissible Gastroenteritis Virus Infection Enhances SGLT1 and GLUT2 Expression to Increase Glucose Uptake.
abstract: Transmissible gastroenteritis virus (TGEV) is a coronavirus that causes villus atrophy, followed by crypt hyperplasia, reduces the activities of intestinal digestive enzymes, and disrupts the absorption of intestinal nutrients. In vivo, TGEV primarily targets and infects intestinal epithelial cells, which play an important role in glucose absorption via the apical and basolateral transporters Na+-dependent glucose transporter 1 (SGLT1) and facilitative glucose transporter 2 (GLUT2), respectively. In this study, we therefore sought to evaluate the effects of TGEV infection on glucose uptake and SGLT1 and GLUT2 expression. Our data demonstrate that infection with TGEV resulted in increased glucose uptake and augmented expression of EGFR, SGLT1 and GLUT2. Moreover, inhibition studies showed that EGFR modulated glucose uptake in control and TGEV infected cells. Finally, high glucose absorption was subsequently found to promote TGEV replication.
authors.affiliation: Veterinary College, Nanjing Agricultural University, Weigang 1, Nanjing, Jiangsu, PR China.
authors.forename: Lei
authors.initials: L
authors.lastname: Dai
authors.affiliation: Veterinary College, Nanjing Agricultural University, Weigang 1, Nanjing, Jiangsu, PR China.
authors.forename: Wei Wei
authors.initials: WW
authors.lastname: Hu
authors.affiliation: Veterinary College, Nanjing Agricultural University, Weigang 1, Nanjing, Jiangsu, PR China.
authors.forename: Lu
authors.initials: L
authors.lastname: Xia
authors.affiliation: Veterinary College, Nanjing Agricultural University, Weigang 1, Nanjing, Jiangsu, PR China.
authors.forename: Mi
authors.initials: M
authors.lastname: Xia
authors.affiliation: Veterinary College, Nanjing Agricultural University, Weigang 1, Nanjing, Jiangsu, PR China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Yang
mesh_terms: D000818:Animals; D004847:Epithelial Cells / Q000378:metabolism; D066246:ErbB Receptors / Q000378:metabolism; D005761:Gastroenteritis, Transmissible, of Swine / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism*; D051273:Glucose Transporter Type 2 / Q000378:metabolism*; D057809:HEK293 Cells; D006801:Humans; D042541:Intracellular Space / Q000378:metabolism; D051379:Mice; D000067996:RAW 264.7 Cells; D051269:Sodium-Glucose Transporter 1 / Q000378:metabolism*; D034421:Sus scrofa; D013552:Swine; D005760:Transmissible gastroenteritis virus / Q000502:physiology*; D014779:Virus Replication
chemical_list: D051273:Glucose Transporter Type 2; D051269:Sodium-Glucose Transporter 1; D066246:ErbB Receptors; D005947:Glucose