title: Arabidopsis plastidial folylpolyglutamate synthetase is required for nitrogen metabolism under nitrate-limited condition in darkness.
abstract: Folates play an important role in plant metabolism. Here we report a T-DNA insertion mutant (atdfb-3) of the plastidial folylpolyglutamate synthetase gene (AtDFB) was defective in folate metabolism and nitrogen metabolism under nitrate-limited conditions in darkness. Exogenous applied 5-formyl-tetrahydrofolate (5-F-THF) completely restored nitrogen content, soluble protein, total amino acids, individual amino acids including Glu, Gln, Asp, Asn, Pro, Arg and Met, nitrate, and endogenous 5-F-THF in atdfb-3 to the wild-type level. At the same time the application of 5-F-THF partially restored the content of Ser and nitrite in the mutant. Taken together, these results indicated that intact folate metabolism was necessary for nitrogen metabolism in Arabidopsis thaliana under nitrate-limited condition in darkness, providing novel insights into function of folate.
authors.affiliation: Fujian Provincial Key Laboratory of Subtropical Plant Physiology and Biochemistry, Fujian Institute of Subtropical Botany, Xiamen, 361006, People's Republic of China. Electronic address: fjsib_mhy@xm.gov.cn.
authors.forename: Hongyan
authors.initials: H
authors.lastname: Meng
authors.affiliation: Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing, 100081, People's Republic of China.
authors.forename: Bosi
authors.initials: B
authors.lastname: Xu
authors.affiliation: Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing, 100081, People's Republic of China; National Key Facility for Crop Gene Resources and Genetic Improvement (NFCRI), Beijing, 100081, People's Republic of China.
authors.forename: Chunyi
authors.initials: C
authors.lastname: Zhang
authors.affiliation: Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing, 100081, People's Republic of China; National Key Facility for Crop Gene Resources and Genetic Improvement (NFCRI), Beijing, 100081, People's Republic of China.
authors.forename: Ling
authors.initials: L
authors.lastname: Jiang
mesh_terms: D017360:Arabidopsis / Q000502:physiology* / Q000528:radiation effects; D003624:Darkness; D008027:Light; D009566:Nitrates / Q000378:metabolism*; D009584:Nitrogen / Q000378:metabolism*; D010453:Peptide Synthases / Q000378:metabolism*; D010788:Photosynthesis / Q000187:drug effects / Q000502:physiology; D018087:Plastids / Q000201:enzymology*; D013312:Stress, Physiological / Q000502:physiology* / Q000528:radiation effects
chemical_list: D009566:Nitrates; D010453:Peptide Synthases; C023103:folylpolyglutamate synthetase; D009584:Nitrogen
keywords: Arabidopsis; Darkness; Folate; Folylpolyglutamate synthetase; Nitrogen metabolism