title: Comparative study on fatty acid metabolism of early stages of two crustacean species: Artemia sp. metanauplii and Grapsus adscensionis zoeae, as live prey for marine animals.
abstract: The present study compared the lipid composition and in vivo capability of Artemia sp. metanauplii (the main live prey used in aquaculture) and Grapsus adscensionis zoeae (as a wild zooplankton model) to metabolise unsaturated fatty acids. The two species were incubated in vivo with 0.3μM of individual [1-14C]fatty acids (FA) including 18:1n-9, 18:2n-6, 18:3n-3, 20:4n-6 (ARA), 20:5n-3 (EPA) and 22:6n-3 (DHA) bound to bovine serum albumin (BSA). Compared to metanauplii, zoeae contained twice the content of polar lipids (PL) and eight-fold the content of long-chain polyunsaturated fatty acids (LC-PUFA). Artemia sp. metanauplii showed increased short chain fatty acid de novo synthesis from beta-oxidation of [1-14C]LC-PUFA, preferentially DHA. Of the LC-PUFA, DHA showed the highest esterification rate into Artemia sp. triacylglycerols. In contrast, in Grapsus zoeae [1-14C]DHA displayed the highest transformation rate into longer chain-length FAs and was preferentially esterified into PL. EPA and ARA, tended to be more easily incorporated and/or retained than DHA in Artemia sp. Moreover, both EPA and ARA were preferentially esterified into Artemia PL, which theoretically would favour their bioavailability to the larvae. In addition to the inherent better nutritional value of Grapsus zoeae due to their intrinsic lipid composition, the changes taking place after the lipid incorporation, point at two distinct models of lipid metabolism that indicate zoeae as a more suitable prey than Artemia sp. for the feeding of marine animals.
authors.affiliation: Departamento de Biología Animal, Edafología y Geología, Universidad de La Laguna, Avenida Astrofísico Francisco Sánchez, 38206, La Laguna, Santa Cruz de Tenerife, Canary Islands, Spain; CCMAR, Centro de Ciências do Mar do Algarve, Universidade do Algarve, Campus de Gambelas, 8005-139, Faro, Portugal; Instituto Español de Oceanografía, Centro Oceanográfico de Canarias, Vía Espaldón no. 1, Dársena pesquera, Parcela no. 8, CP: 38180 Santa Cruz de Tenerife, Canary Islands, Spain. Electronic address: dfbreis@ualg.pt.
authors.forename: Diana B
authors.initials: DB
authors.lastname: Reis
authors.affiliation: Departamento de Biología Animal, Edafología y Geología, Universidad de La Laguna, Avenida Astrofísico Francisco Sánchez, 38206, La Laguna, Santa Cruz de Tenerife, Canary Islands, Spain.
authors.forename: Nieves G
authors.initials: NG
authors.lastname: Acosta
authors.affiliation: Instituto Español de Oceanografía, Centro Oceanográfico de Canarias, Vía Espaldón no. 1, Dársena pesquera, Parcela no. 8, CP: 38180 Santa Cruz de Tenerife, Canary Islands, Spain.
authors.forename: Eduardo
authors.initials: E
authors.lastname: Almansa
authors.affiliation: Instituto de Acuicultura Torre de la Sal (IATS-CSIC), 12595, Ribera de Cabanes, Castellón, Spain.
authors.forename: Juan C
authors.initials: JC
authors.lastname: Navarro
authors.affiliation: Institute of Aquaculture, School of Natural Sciences, University of Stirling, Stirling FK9 4LA, Scotland, UK.
authors.forename: Douglas R
authors.initials: DR
authors.lastname: Tocher
authors.affiliation: CCMAR, Centro de Ciências do Mar do Algarve, Universidade do Algarve, Campus de Gambelas, 8005-139, Faro, Portugal.
authors.forename: José P
authors.initials: JP
authors.lastname: Andrade
authors.affiliation: CCMAR, Centro de Ciências do Mar do Algarve, Universidade do Algarve, Campus de Gambelas, 8005-139, Faro, Portugal.
authors.forename: António V
authors.initials: AV
authors.lastname: Sykes
authors.affiliation: Departamento de Biología Animal, Edafología y Geología, Universidad de La Laguna, Avenida Astrofísico Francisco Sánchez, 38206, La Laguna, Santa Cruz de Tenerife, Canary Islands, Spain.
authors.forename: Covadonga
authors.initials: C
authors.lastname: Rodríguez
mesh_terms: D000818:Animals; D001156:Artemia / Q000378:metabolism*; D001692:Biological Transport; D004951:Esterification; D005231:Fatty Acids, Unsaturated / Q000737:chemistry / Q000378:metabolism*; D020387:Food Chain; D007814:Larva / Q000254:growth & development
chemical_list: D005231:Fatty Acids, Unsaturated
keywords: Artemia sp. metanauplii; Grapsus adscensionis zoeae; Lipid metabolism; Marine larvae; Unsaturated fatty acids