title: Ubiquitination of ERMES components by the E3 ligase Rsp5 is involved in mitophagy.
abstract: Mitochondria are dynamic organelles that undergo permanent fission and fusion events. These processes play an essential role in maintaining normal cellular function. In the yeast Saccharomyces cerevisiae, the endoplasmic reticulum-mitochondrial encounter structure (ERMES) is a marker of sites of mitochondrial division, but it is also involved in a plethora of other mitochondrial functions. However, it remains unclear how these different functions are regulated. We show here that Mdm34 and Mdm12, 2 components of ERMES, are ubiquitinated by the E3 ligase Rsp5. This ubiquitination is not involved in mitochondrial dynamics or in the distribution and turnover of ERMES. Nevertheless, the ubiquitination of Mdm34 and Mdm12 was required for efficient mitophagy. We thus report here the first identification of ubiquitinated substrates participating in yeast mitophagy.
authors.affiliation: a UMR8226, CNRS/UPMC, Sorbonne Université, Institut de Biologie Physico-Chimique , Paris , France.
authors.forename: Naïma
authors.initials: N
authors.lastname: Belgareh-Touzé
authors.affiliation: a UMR8226, CNRS/UPMC, Sorbonne Université, Institut de Biologie Physico-Chimique , Paris , France.
authors.forename: Laetitia
authors.initials: L
authors.lastname: Cavellini
authors.affiliation: a UMR8226, CNRS/UPMC, Sorbonne Université, Institut de Biologie Physico-Chimique , Paris , France.
authors.forename: Mickael M
authors.initials: MM
authors.lastname: Cohen
mesh_terms: D020816:Amino Acid Motifs; D001343:Autophagy; D004721:Endoplasmic Reticulum / Q000378:metabolism; D056827:Endosomal Sorting Complexes Required for Transport / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D008565:Membrane Proteins / Q000737:chemistry*; D008928:Mitochondria / Q000378:metabolism; D063154:Mitochondrial Dynamics; D024101:Mitochondrial Proteins / Q000737:chemistry*; D063306:Mitophagy; D010957:Plasmids / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry* / Q000378:metabolism*; D043743:Ubiquitin-Protein Ligase Complexes / Q000378:metabolism*; D044767:Ubiquitin-Protein Ligases / Q000378:metabolism*; D054875:Ubiquitination
chemical_list: D056827:Endosomal Sorting Complexes Required for Transport; C543343:Mdm12 protein, S cerevisiae; D008565:Membrane Proteins; D024101:Mitochondrial Proteins; D029701:Saccharomyces cerevisiae Proteins; C543344:mdm34 protein, S cerevisiae; D043743:Ubiquitin-Protein Ligase Complexes; D044767:Ubiquitin-Protein Ligases; C469287:RSP5 protein, S cerevisiae
keywords: ER; ERMES; Mdm12; Mdm34; Rsp5; S. cerevisiae; mitochondria; mitophagy; ubiquitin