title: Inductors and regulatory properties of the genomic island-associated fru2metabolic operon of Streptococcus agalactiae.
abstract: The fru2metabolic operon of Streptococcus agalactiae encodes the phosphoenolpyruvate:carbohydrate phosphotransferase system (PTS) enzyme II complex Fru2(EIIBFru2, EIIAFru2, and EIICFru2); Fru2R, a transcriptional activator with PTS regulatory domains (PRDs); a d-allulose-6-phosphate 3-epimerase; a transaldolase; and a transketolase. We showed that the transcription of fru2is induced during the stationary phase of growth in complex media and during incubation in human cerebrospinal or amniotic fluids. d-allose and d-ribose are environmental signals governing this induction. PTSFru2is involved in the activation of the fru2promoter, and the histidine-67 of EIIAFru2and the cysteine-9 of EIIBFru2are important for this function. The activation of fru2is also controlled by Fru2R. The histidine-243 in the PRD1domain, the histidine-323 in the PRD2domain, the cysteine-400 in the EIIB-like domain, and the histidine-549 in the EIIA-like domain are important for the function of Fru2R. Fru2R binds to a DNA region containing palindromic sequences upstream of the identified transcriptional start site. EIIBFru2interacts physically with the C-terminal part of Fru2R (expressing the EIIB-like and EIIA-like motifs) and with EIIAFru2. We propose a model of regulation of fru2depending on the presence of an activatory carbohydrate in the growth medium.
authors.affiliation: Bactéries et Risque Materno-Foetal, UMR1282 Infectiologie et Santé Publique, Université François Rabelais, INRA, Tours, F-37032, France.
authors.forename: Kévin
authors.initials: K
authors.lastname: Patron
authors.affiliation: Bactéries et Risque Materno-Foetal, UMR1282 Infectiologie et Santé Publique, Université François Rabelais, INRA, Tours, F-37032, France.
authors.forename: Philippe
authors.initials: P
authors.lastname: Gilot
authors.affiliation: Bactéries et Risque Materno-Foetal, UMR1282 Infectiologie et Santé Publique, Université François Rabelais, INRA, Tours, F-37032, France.
authors.forename: Vanessa
authors.initials: V
authors.lastname: Rong
authors.affiliation: Bactéries et Risque Materno-Foetal, UMR1282 Infectiologie et Santé Publique, Université François Rabelais, INRA, Tours, F-37032, France.
authors.forename: Aurélia
authors.initials: A
authors.lastname: Hiron
authors.affiliation: Bactéries et Risque Materno-Foetal, UMR1282 Infectiologie et Santé Publique, Université François Rabelais, INRA, Tours, F-37032, France.|Service de Bactériologie-Virologie-Hygiène, Centre Hospitalier Universitaire de Tours, Tours, F-37044, France.
authors.forename: Laurent
authors.initials: L
authors.lastname: Mereghetti
authors.affiliation: Bactéries et Risque Materno-Foetal, UMR1282 Infectiologie et Santé Publique, Université François Rabelais, INRA, Tours, F-37032, France.
authors.forename: Emilie
authors.initials: E
authors.lastname: Camiade
mesh_terms: D000653:Amniotic Fluid / Q000378:metabolism; D002555:Cerebrospinal Fluid / Q000378:metabolism; D003470:Culture Media / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial / Q000235:genetics*; D005809:Genes, Regulator / Q000235:genetics*; D044404:Genomic Islands / Q000235:genetics; D005947:Glucose / Q000378:metabolism*; D006801:Humans; D009876:Operon / Q000235:genetics; D010731:Phosphoenolpyruvate Sugar Phosphotransferase System / Q000235:genetics*; D011401:Promoter Regions, Genetic / Q000235:genetics; D012266:Ribose / Q000378:metabolism*; D013292:Streptococcus agalactiae / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D015533:Transcriptional Activation / Q000235:genetics
chemical_list: D003470:Culture Media; D012266:Ribose; D010731:Phosphoenolpyruvate Sugar Phosphotransferase System; D005947:Glucose