title: Deletion of Pr130 Interrupts Cardiac Development in Zebrafish.
abstract: Protein phosphatase 2 regulatory subunit B, alpha (PPP2R3A), a regulatory subunit of protein phosphatase 2A (PP2A), is a major serine/threonine phosphatase that regulates crucial function in development and growth. Previous research has implied thatPPP2R3Awas involved in heart failure, and PR130, the largest transcription ofPPP2R3A, functioning in the calcium release of sarcoplasmic reticulum (SR), plays an important role in the excitation-contraction (EC) coupling. To obtain a better understanding of PR130 functions in myocardium and cardiac development, twopr130-deletion zebrafish lines were generated using clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated proteins (Cas) system.Pr130-knockout zebrafish exhibited cardiac looping defects and decreased cardiac function (decreased fractional area and fractional shortening). Hematoxylin and eosin (H&amp;E) staining demonstrated reduced cardiomyocytes. Subsequent transmission electron microscopy revealed that the bright and dark bands were narrowed and blurred, the Z- and M-lines were fogged, and the gaps between longitudinal myocardial fibers were increased. Additionally, increased apoptosis was observed in cardiomyocyte inpr130-knockout zebrafish compared to wild-type (WT). Taken together, our results suggest thatpr130is required for normal myocardium formation and efficient cardiac contractile function.
authors.affiliation: Center for Gene Diagnosis, Zhongnan Hospital of Wuhan University, Wuhan 430071, China. manbuyunhailzh@163.com.
authors.forename: Jie
authors.initials: J
authors.lastname: Yang
authors.affiliation: Center for Gene Diagnosis, Zhongnan Hospital of Wuhan University, Wuhan 430071, China. lzu@whu.edu.cn.
authors.forename: Zuhua
authors.initials: Z
authors.lastname: Li
authors.affiliation: Department of Cardiology, Zhongnan Hospital of Wuhan University, Wuhan 430071, China. sunshine8789@yeah.net.
authors.forename: Xuedong
authors.initials: X
authors.lastname: Gan
authors.affiliation: Key Laboratory of Aquatic Biodiversity and Conservation of Chinese Academy of Sciences, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China. zhaigang19@126.com.
authors.forename: Gang
authors.initials: G
authors.lastname: Zhai
authors.affiliation: Center for Gene Diagnosis, Zhongnan Hospital of Wuhan University, Wuhan 430071, China. flower198901@163.com.
authors.forename: Jiajia
authors.initials: J
authors.lastname: Gao
authors.affiliation: Center for Gene Diagnosis, Zhongnan Hospital of Wuhan University, Wuhan 430071, China. perkinshine@yeah.net.
authors.forename: Chenling
authors.initials: C
authors.lastname: Xiong
authors.affiliation: Center for Gene Diagnosis, Zhongnan Hospital of Wuhan University, Wuhan 430071, China. happytime19881@yeah.net.
authors.forename: Xueping
authors.initials: X
authors.lastname: Qiu
authors.affiliation: Center for Gene Diagnosis, Zhongnan Hospital of Wuhan University, Wuhan 430071, China. xbwang2013@whu.edu.cn.
authors.forename: Xuebin
authors.initials: X
authors.lastname: Wang
authors.affiliation: Key Laboratory of Aquatic Biodiversity and Conservation of Chinese Academy of Sciences, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China. zyin@ihb.ac.cn.
authors.forename: Zhan
authors.initials: Z
authors.lastname: Yin
authors.affiliation: Center for Gene Diagnosis, Zhongnan Hospital of Wuhan University, Wuhan 430071, China. zhengfang@whu.edu.cn.
authors.forename: Fang
authors.initials: F
authors.lastname: Zheng
mesh_terms: D000818:Animals; D017209:Apoptosis; D017353:Gene Deletion; D006321:Heart / Q000196:embryology*; D009200:Myocardial Contraction; D009206:Myocardium / Q000201:enzymology / Q000473:pathology; D032383:Myocytes, Cardiac / Q000201:enzymology; D054648:Protein Phosphatase 2 / Q000235:genetics*; D015027:Zebrafish
chemical_list: D054648:Protein Phosphatase 2
keywords: CRISPR-Cas9; cardiac development; pr130; zebrafish