title: Lipoprotein lipase and phospholipid transfer protein overexpression in human glioma cells and their effect on cell growth, apoptosis, and migration.
abstract: Glioma is one of the common tumors in brain. The expression level of lipoprotein lipase (LPL) or phospholipid transfer protein (PLTP) may influence glioma progression and its relationship with clinical and pathological parameters. The clinical significance of LPL or PLTP expression in glioma has not been established. In the present study, the LPL and PLTP levels in glioma tumors were investigated and the relationship between the LPL and PLTP level and the grade of malignant glioma was analyzed, with the aim to provide new ideas for the diagnosis and treatment of gliomas in clinical and basic research settings. LPL and PLTP mRNA and protein levels were significantly higher in Grade IV glioma than those in the lower grade tumors (P < 0.01). Double immunofluorescent staining showed that the levels of LPL and PLTP were significantly associated with the pathological grade of glioma (P = 0.005). The levels of LPL and PLTP were increased with the shortened survival of glioma patients (P < 0.001). Knockdown of LPL and PLTP led to decreased cell growth and migration but increased apoptosis in vitro Additionally, cell cycle-related cyclins and their partners were found to be down-regulated while cyclin-dependent kinase inhibitors p16, p21, and Rb were up-regulated. Furthermore, knockdown of LPL or PLTP resulted in the up-regulation of pro-apoptotic molecules and the down-regulation of anti-apoptotic molecules. Ablation of LPL or PLTP in U251 cells resulted in the down-regulation of epithelial mesenchymal transition markers and invasion molecules matrix metalloproteinases. LPL and PLTP appear to be novel glioma-associated proteins and play a role in the progression of human glioma.
authors.affiliation: Department of Human Anatomy, Histology and Embryology, School of Basic Medical Sciences, Xi'an Jiaotong University Health Science Center, Xi'an 710061, China.
authors.forename: Weijiang
authors.initials: W
authors.lastname: Dong
authors.affiliation: Department of Pathology, the First Affiliated Hospital of Xi'an Jiaotong University, Xi'an 710061, China.
authors.forename: Huilin
authors.initials: H
authors.lastname: Gong
authors.affiliation: Department of Pathology, the First Affiliated Hospital of Xi'an Jiaotong University, Xi'an 710061, China.
authors.forename: Guanjun
authors.initials: G
authors.lastname: Zhang
authors.affiliation: Northwest Lipid Metabolism and Diabetes Research Laboratories, Department of Medicine, School of Medicine, University of Washington, Seattle, 98109 WA.
authors.forename: Simona
authors.initials: S
authors.lastname: Vuletic
authors.affiliation: Northwest Lipid Metabolism and Diabetes Research Laboratories, Department of Medicine, School of Medicine, University of Washington, Seattle, 98109 WA.
authors.forename: John
authors.initials: J
authors.lastname: Albers
authors.affiliation: Department of Pathology, the First Affiliated Hospital of Xi'an Jiaotong University, Xi'an 710061, China.
authors.forename: Jiaojiao
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Department of Pathology, the First Affiliated Hospital of Xi'an Jiaotong University, Xi'an 710061, China.
authors.forename: Hua
authors.initials: H
authors.lastname: Liang
authors.affiliation: Department of Pathology, the First Affiliated Hospital of Xi'an Jiaotong University, Xi'an 710061, China.
authors.forename: Yanxia
authors.initials: Y
authors.lastname: Sui
authors.affiliation: Hospital of Nephrology, the First Affiliated Hospital of Xi'an Jiaotong University, Xi'an 710061, China jzheng@xjtu.edu.cn.
authors.forename: Jin
authors.initials: J
authors.lastname: Zheng
mesh_terms: D017209:Apoptosis; D001932:Brain Neoplasms / Q000378:metabolism* / Q000473:pathology; D002455:Cell Division; D045744:Cell Line, Tumor; D002465:Cell Movement; D005910:Glioma / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D008071:Lipoprotein Lipase / Q000235:genetics / Q000378:metabolism*; D049294:Phospholipid Transfer Proteins / Q000235:genetics / Q000378:metabolism*; D034741:RNA, Small Interfering / Q000235:genetics
chemical_list: D049294:Phospholipid Transfer Proteins; D034741:RNA, Small Interfering; D008071:Lipoprotein Lipase
keywords: LPL; PLTP; apoptosis; colony formation; glioma; migration