title: Gene expression profiles of human subcutaneous and visceral adipose-derived stem cells.
abstract: Subcutaneous and visceral adipose tissues show a different risk effect on metabolic disorders because they have distinct cellular properties. We isolated stem cells from the separate human adipose tissues to investigate that subcutaneous and visceral fat depots have metabolic differences. Adipose-derived stem cells (ASCs) were characterized by immunophenotype and differentiation potentials into adipogenic, osteogenic, and chondrogenic lineages. Although subcutaneous and visceral ASCs (S-ASC and V-ASC) express same surface markers (CD31-, CD34-, CD45-, CD73+, CD90+, and CD105+) and have differentiation potentials, S-ASCs had higher capacity to proliferate and to differentiate into adipogenic lineage than V-ASCs. Next, we identified that S-ASC and V-ASC were genetically distinct based on microarray analysis. Among a total of 810 genes detected in ASCs of both depots, the differentially expressed genes were involved in energy and lipid metabolism. These data show the existence of the intrinsic difference between S-ASC and V-ASC and suggest the differences of anatomically separated adipose tissue. On the basis of the differentially expressed gene profiles between S-ASC and V-ASC, we suggested significant evidence that adipose tissues originating from different anatomic regions are distinguished at the level of the undifferentiated stem cells such as mature adipocytes. V-ASCs had the upregulated clusters of genes related to lipid biosynthesis and metabolism. By contrast, S-ASCs highly expressed genes involved in DNA-dependent transcription, contributing to proliferation. We provide further insights for ASCs with the different origins to understand fat accumulation and distribution and a possibility of ASCs as a therapeutic target against metabolic disorders or cancer.
authors.affiliation: Cancer Research Institute, College of Medicine, Seoul National University, Seoul, South Korea.|Nano System Institute, Seoul National University, Seoul, South Korea.
authors.forename: Boyun
authors.initials: B
authors.lastname: Kim
authors.affiliation: Cancer Research Institute, College of Medicine, Seoul National University, Seoul, South Korea.|WCU Biomodulation, Department of Agricultural Biotechnology, Seoul National University, Seoul, South Korea.
authors.forename: Boram
authors.initials: B
authors.lastname: Lee
authors.affiliation: Department of Obstetrics and Gynecology, Cheil General Hospital & Women's Healthcare Center, Dankook University College of Medicine, Seoul, South Korea.
authors.forename: Mi-Kyung
authors.initials: MK
authors.lastname: Kim
authors.affiliation: Department of Marine Bio-materials and Aquaculture, College of Fisheries Sciences, Pukyong National University, Busan, South Korea.
authors.forename: Seung Pyo
authors.initials: SP
authors.lastname: Gong
authors.affiliation: Department of Obstetrics and Gynecology, Seoul National University College of Medicine, Seoul, South Korea.
authors.forename: Noh Hyun
authors.initials: NH
authors.lastname: Park
authors.affiliation: Department of Obstetrics and Gynecology, Seoul National University College of Medicine, Seoul, South Korea.
authors.forename: Hyun Hoon
authors.initials: HH
authors.lastname: Chung
authors.affiliation: Department of Obstetrics and Gynecology, Seoul National University College of Medicine, Seoul, South Korea.
authors.forename: Hee Seung
authors.initials: HS
authors.lastname: Kim
authors.affiliation: Department of Obstetrics and Gynecology, Seoul National University College of Medicine, Seoul, South Korea.
authors.forename: Jae Hong
authors.initials: JH
authors.lastname: No
authors.affiliation: Samsung Medical Center, Samsung Genome Institute, Seoul, South Korea.
authors.forename: Woong Yang
authors.initials: WY
authors.lastname: Park
authors.affiliation: College of Pharmacy, Sunchon National University, Jeonnam, South Korea.
authors.forename: Ae Kyung
authors.initials: AK
authors.lastname: Park
authors.affiliation: WCU Biomodulation, Department of Agricultural Biotechnology, Seoul National University, Seoul, South Korea.
authors.forename: Jeong Mook
authors.initials: JM
authors.lastname: Lim
authors.affiliation: Cancer Research Institute, College of Medicine, Seoul National University, Seoul, South Korea.|WCU Biomodulation, Department of Agricultural Biotechnology, Seoul National University, Seoul, South Korea.|Department of Obstetrics and Gynecology, Seoul National University College of Medicine, Seoul, South Korea.
authors.forename: Yong Sang
authors.initials: YS
authors.lastname: Song
mesh_terms: D000328:Adult; D000368:Aged; D002469:Cell Separation; D020869:Gene Expression Profiling; D063990:Gene Ontology; D006801:Humans; D050152:Intra-Abdominal Fat / Q000166:cytology*; D008875:Middle Aged; D060888:Real-Time Polymerase Chain Reaction; D015203:Reproducibility of Results; D013234:Stem Cells / Q000378:metabolism*; D050151:Subcutaneous Fat / Q000166:cytology*; D015854:Up-Regulation / Q000235:genetics
keywords: adipose tissue; adipose-derived stem cell; metabolism; microarray; subcutaneous fat; visceral fat