title: Intracellular synthesis of gold nanoparticles with antioxidant activity by probiotic Lactobacillus kimchicus DCY51Tisolated from Korean kimchi.
abstract: A straightforward synthesis of gold nanoparticles (AuNps) is achieved by novel probiotic Lactobacillus kimchicus DCY51Tisolated from Korean kimchi via an intracellular membrane-bound mechanism. The bioreduction of HAuCl4into AuNps was verified by ultraviolet-visible spectrophotometry at ∼540nm. AuNps were spherical with varying sizes of 5-30nm. AuNps maintained an average crystallite size of 13nm and demonstrated long-term stability in physiological buffer and biological media. Furthermore, the protective capping layer consisted of amino acid residues and surface-bound proteins rendered them non-toxic to murine macrophage (RAW264.7) and human colorectal adenocarcinoma (HT29) cell lines. Finally, biosynthesized AuNps served as superior free radical scavengers against 2,2-diphenyl-1-picrylhydrazyl (DPPH) in contrast to their corresponding gold salt. In short, this green synthesis is cost-effective and advantageous for the development of a new class of probiotics mediated and non-toxic carriers in targeted drug delivery systems, cancer diagnostic, photothermal therapy, biosensing, and medical imaging.
authors.affiliation: Graduate School of Biotechnology, College of Life Science, Kyung Hee University, Republic of Korea.
authors.forename: Josua
authors.initials: J
authors.lastname: Markus
authors.affiliation: Graduate School of Biotechnology, College of Life Science, Kyung Hee University, Republic of Korea.
authors.forename: Ramya
authors.initials: R
authors.lastname: Mathiyalagan
authors.affiliation: Department of Oriental Medicine Biotechnology and Ginseng Bank, College of Life Science, Kyung Hee University, Republic of Korea. Electronic address: yeonjukim@khu.ac.kr.
authors.forename: Yeon-Ju
authors.initials: YJ
authors.lastname: Kim
authors.affiliation: Graduate School of Biotechnology, College of Life Science, Kyung Hee University, Republic of Korea.
authors.forename: Ragavendran
authors.initials: R
authors.lastname: Abbai
authors.affiliation: Department of Oriental Medicine Biotechnology and Ginseng Bank, College of Life Science, Kyung Hee University, Republic of Korea.
authors.forename: Priyanka
authors.initials: P
authors.lastname: Singh
authors.affiliation: Graduate School of Biotechnology, College of Life Science, Kyung Hee University, Republic of Korea.
authors.forename: Sungeun
authors.initials: S
authors.lastname: Ahn
authors.affiliation: Graduate School of Biotechnology, College of Life Science, Kyung Hee University, Republic of Korea.
authors.forename: Zuly Elizabeth Jimenez
authors.initials: ZEJ
authors.lastname: Perez
authors.affiliation: Graduate School of Biotechnology, College of Life Science, Kyung Hee University, Republic of Korea.
authors.forename: Joon
authors.initials: J
authors.lastname: Hurh
authors.affiliation: Graduate School of Biotechnology, College of Life Science, Kyung Hee University, Republic of Korea; Department of Oriental Medicine Biotechnology and Ginseng Bank, College of Life Science, Kyung Hee University, Republic of Korea. Electronic address: dcyang@khu.ac.kr.
authors.forename: Deok Chun
authors.initials: DC
authors.lastname: Yang
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology; D001709:Biotechnology; D005516:Food Microbiology; D016166:Free Radical Scavengers / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D006046:Gold / Q000737:chemistry*; D055772:Green Chemistry Technology; D019073:HT29 Cells; D006801:Humans; D007778:Lactobacillus / Q000145:classification / Q000302:isolation & purification / Q000378:metabolism*; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D051379:Mice; D036103:Nanotechnology; D010084:Oxidation-Reduction; D010316:Particle Size; D010802:Phylogeny; D019936:Probiotics / Q000378:metabolism; D000067996:RAW 264.7 Cells; D056910:Republic of Korea; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D000975:Antioxidants; D016166:Free Radical Scavengers; D006046:Gold
keywords: Antioxidant activity; Biomedical application; Cytotoxicity; Gold nanoparticles; Probiotics; Spectroscopy