title: p38α has an important role in antigen cross-presentation by dendritic cells.
abstract: The role of the p38 signaling pathway in the innate and adaptive immune responses has been well documented, especially in inflammatory cytokine production by dendritic cells (DCs). However, whether the p38 signaling pathway affects the important antigen (Ag) presentation function of DCs remains largely unknown. In this study, we reported that the deletion of p38α resulted in an impaired cross-presentation ability of CD8+conventional DCs (cDCs) and a reduction in the direct presentation ability of CD8-cDCs ex vivo. Further study revealed that p38α had a crucial role in Ag processing by CD8+cDCs but did not affect the Ag uptake or co-stimulation of T cells. Moreover, p38α deficiency led to reduced cross-priming of T cells in vivo. The production of the IL-12p40 and IL-12p70 cytokines by p38α-deficient cDCs was also significantly reduced. Our study identified a new role for p38α in modulating the important antigen cross-presentation function of DCs.
authors.affiliation: Institute for Immunology, School of Medicine, Tsinghua University, Beijing 100084, China.
authors.forename: Yifan
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: State Key Laboratory of Cellular Stress Biology, School of Life Sciences, Xiamen University, Xiamen 361005, Fujian, China.
authors.forename: Jianfeng
authors.initials: J
authors.lastname: Wu
authors.affiliation: State Key Laboratory of Biomembrane, National Engineering Laboratory for Anti-tumor Therapeutics, School of Medicine, Tsinghua University, Beijing 100084, China.
authors.forename: Chunxiao
authors.initials: C
authors.lastname: Liu
authors.affiliation: Institute for Immunology, School of Medicine, Tsinghua University, Beijing 100084, China.
authors.forename: Xueheng
authors.initials: X
authors.lastname: Guo
authors.affiliation: Institute for Immunology, School of Medicine, Tsinghua University, Beijing 100084, China.
authors.forename: Xinyi
authors.initials: X
authors.lastname: Zhu
authors.affiliation: Institute for Immunology, School of Medicine, Tsinghua University, Beijing 100084, China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Yao
authors.affiliation: Institute for Immunology, School of Medicine, Tsinghua University, Beijing 100084, China.
authors.forename: Yuhao
authors.initials: Y
authors.lastname: Jiao
authors.affiliation: State Key Laboratory of Cellular Stress Biology, School of Life Sciences, Xiamen University, Xiamen 361005, Fujian, China.
authors.forename: Peng
authors.initials: P
authors.lastname: He
authors.affiliation: State Key Laboratory of Cellular Stress Biology, School of Life Sciences, Xiamen University, Xiamen 361005, Fujian, China.
authors.forename: Jiahuai
authors.initials: J
authors.lastname: Han
authors.affiliation: Institute for Immunology, School of Medicine, Tsinghua University, Beijing 100084, China.|Tsinghua-Peking Joint Center for Life Sciences, Beijing 100084, China.
authors.forename: Li
authors.initials: L
authors.lastname: Wu
mesh_terms: D000818:Animals; D016827:CD8 Antigens / Q000378:metabolism; D002478:Cells, Cultured; D045142:Cross-Priming; D003713:Dendritic Cells / Q000276:immunology*; D015395:Histocompatibility Antigens Class I / Q000378:metabolism; D018664:Interleukin-12 / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D048308:Mitogen-Activated Protein Kinase 14 / Q000235:genetics / Q000378:metabolism*; D011485:Protein Binding; D059748:Proteolysis; D015398:Signal Transduction; D013601:T-Lymphocytes / Q000276:immunology*
chemical_list: D016827:CD8 Antigens; D015395:Histocompatibility Antigens Class I; D018664:Interleukin-12; D048308:Mitogen-Activated Protein Kinase 14