title: Swimming Exercise Alleviated Insulin Resistance by Regulating Tripartite Motif Family Protein 72 Expression and AKT Signal Pathway in Sprague-Dawley Rats Fed with High-Fat Diet.
abstract: We aimed to investigate whether swimming exercise could improve insulin resistance (IR) by regulating tripartite motif family protein 72 (TRIM72) expression and AKT signal pathway in rats fed with high-fat diet. Five-week-old rats were classified into 3 groups: standard diet as control (CON), high-fat diet (HFD), and HFD plus swimming exercise (Ex-HFD). After 8 weeks, glucose infusion rate (GIR), markers of oxidative stress, mRNA and protein expression of TRIM72, protein of IRS, p-AKTSer473, and AKT were determined in quadriceps muscles. Compared with HFD, the GIR, muscle SOD, and GSH-Px were significantly increased (p< 0.05, resp.), whereas muscle MDA and 8-OHdG levels were significantly decreased (p< 0.05 andp< 0.01) in Ex-HFD. Expression levels of TRIM72 mRNA and protein in muscles were significantly reduced (p< 0.05 andp< 0.01), whereas protein expression levels of IRS-1, p-AKTSer473, and AKT were significantly increased in Ex-HFD compared with HFD (p< 0.01,p< 0.01, andp< 0.05). These results suggest that an 8-week swimming exercise improves HFD-induced insulin resistance maybe through a reduction of TRIM72 in skeletal muscle and enhancement of AKT signal transduction.
authors.affiliation: College of Physical Education, Shanghai Normal University, Shanghai 200234, China.
authors.forename: Jie
authors.identifier: 0000-0002-9604-2875
authors.initials: J
authors.lastname: Qi
authors.affiliation: Department of Food Science and Nutrition, Zhejiang University, Hangzhou 310053, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Yang
authors.affiliation: Rehabilitation College of Gannan Medical University, Jiangxi 341000, China.
authors.forename: Cailing
authors.identifier: 0000-0002-9143-5426
authors.initials: C
authors.lastname: Ren
authors.affiliation: College of Physical Education, Yangzhou University, Jiangsu 225009, China.
authors.forename: Jian
authors.identifier: 0000-0002-3612-5230
authors.initials: J
authors.lastname: Fu
authors.affiliation: College of Physical Education, Shanghai Normal University, Shanghai 200234, China.
authors.forename: Jun
authors.identifier: 0000-0003-1747-1020
authors.initials: J
authors.lastname: Zhang
mesh_terms: D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D059305:Diet, High-Fat; D005230:Fatty Acids, Nonesterified / Q000378:metabolism; D007328:Insulin / Q000097:blood; D007333:Insulin Resistance / Q000502:physiology*; D008297:Male; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism*; D018384:Oxidative Stress / Q000502:physiology*; D010766:Phosphorylation; D010805:Physical Conditioning, Animal / Q000502:physiology*; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism*; D052097:Quadriceps Muscle / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D015398:Signal Transduction / Q000235:genetics*; D013550:Swimming
chemical_list: D001786:Blood Glucose; D005230:Fatty Acids, Nonesterified; D007328:Insulin; D009419:Nerve Tissue Proteins; D051057:Proto-Oncogene Proteins c-akt