title: Molecular epidemiology of carbapenemase-producing Enterobacteriaceae infection/colonisation in a hospital in Madrid.
abstract: A description is presented on the molecular epidemiology of carbapenemase-producing enterobacteriaceae infection in a tertiary hospital.
  
  
  
  A study was made on all the carbapenemase-producing enterobacteriaceae isolations obtained between February 2015 and March 2016 in the Hospital Universitario 12 de Octubre (Madrid). Phenotypic and molecular methods were used.
  
  
  
  A total of 7 bacterial species were identified, with the majority being Klebsiella pneumoniae (K. pneumoniae) (78.9%) and Enterobacter cloacae (E. cloacae) (16.4%). The resistance of K. pneumoniae and E. cloacae for carbapenems was 88.7 and 88.6% for ertapenem, 21.4 and 54.3% for imipenem, and 20.8 and 34.3% for meropenem, respectively. The most frequent carbapenemase type was OXA-48 (91.1%) and VIM (71.4%) in E. cloacae. A total of 9K. pneumoniae clonal types were identified, including a majority pertaining to the sequence type ST11. In E. cloacae, 16 clonal types were identified.
  
  
  
  The current increase in carbapenemase-producing enterobacteriaceae is mainly due to the spread of OXA-48-producing K. pneumoniae.
authors.affiliation: Servicio de Microbiología Clínica, Hospital Universitario 12 de Octubre, Madrid, España. Electronic address: patriciabg1984@gmail.com.
authors.forename: Patricia
authors.initials: P
authors.lastname: Brañas
authors.affiliation: Servicio de Microbiología Clínica, Hospital Universitario 12 de Octubre, Madrid, España.
authors.forename: Marta
authors.initials: M
authors.lastname: Gil
authors.affiliation: Servicio de Microbiología Clínica, Hospital Universitario 12 de Octubre, Madrid, España.
authors.forename: Jennifer
authors.initials: J
authors.lastname: Villa
authors.affiliation: Servicio de Microbiología Clínica, Hospital Universitario 12 de Octubre, Madrid, España.
authors.forename: María Ángeles
authors.initials: MÁ
authors.lastname: Orellana
authors.affiliation: Servicio de Microbiología Clínica, Hospital Universitario 12 de Octubre, Madrid, España.
authors.forename: Fernando
authors.initials: F
authors.lastname: Chaves
mesh_terms: D001426:Bacterial Proteins / Q000032:analysis* / Q000235:genetics; D003428:Cross Infection / Q000453:epidemiology / Q000382:microbiology*; D024901:Drug Resistance, Multiple, Bacterial / Q000235:genetics*; D016972:Enterobacter cloacae / Q000201:enzymology / Q000235:genetics / Q000302:isolation & purification; D004755:Enterobacteriaceae / Q000145:classification / Q000201:enzymology / Q000235:genetics / Q000302:isolation & purification*; D004756:Enterobacteriaceae Infections / Q000453:epidemiology / Q000382:microbiology*; D006785:Hospitals, University; D006801:Humans; D007710:Klebsiella Infections / Q000453:epidemiology / Q000382:microbiology; D007711:Klebsiella pneumoniae / Q000201:enzymology / Q000235:genetics / Q000302:isolation & purification; D008826:Microbial Sensitivity Tests; D017720:Molecular Epidemiology; D012189:Retrospective Studies; D012621:Seasons; D013030:Spain / Q000453:epidemiology; D062606:Tertiary Care Centers; D018440:beta-Lactam Resistance / Q000235:genetics*; D001618:beta-Lactamases / Q000032:analysis* / Q000235:genetics
chemical_list: D001426:Bacterial Proteins; D001618:beta-Lactamases; C063912:carbapenemase; C108449:oxacillinase
keywords: Carbapenemasas; Carbapenemases; Enterobacteria; Enterobacterias; Epidemiología molecular; Klebsiella pneumoniae; Molecular epidemiology; OXA-48