title: Host-Derived Biomarkers for Risk Assessment of Invasive Fungal Diseases.
abstract: Invasive fungal diseases are major complications associated with the treatment of hematologic malignancies. The integration of host-derived biomarkers into clinical processes to predict the risk and progression of fungal disease is a promising approach in immunocompromised patients. Recent insights into human antifungal immunity have highlighted the remarkable influence of host genetics in modulating susceptibility to infection. In this chapter, we describe protocols to examine human genetic variation and to assess its functional consequences using the pattern recognition receptor PTX3 as an example.
authors.affiliation: Life and Health Sciences Research Institute (ICVS), School of Health Sciences, University of Minho, Campus de Gualtar, Braga, 4710-057, Portugal.|ICVS/3B's-PT Government Associate Laboratory, Braga/Guimarães, Portugal.
authors.forename: Cristina
authors.initials: C
authors.lastname: Cunha
authors.affiliation: Life and Health Sciences Research Institute (ICVS), School of Health Sciences, University of Minho, Campus de Gualtar, Braga, 4710-057, Portugal.|ICVS/3B's-PT Government Associate Laboratory, Braga/Guimarães, Portugal.
authors.forename: Samuel M
authors.initials: SM
authors.lastname: Gonçalves
authors.affiliation: Life and Health Sciences Research Institute (ICVS), School of Health Sciences, University of Minho, Campus de Gualtar, Braga, 4710-057, Portugal. agostinhocarvalho@ecsaude.uminho.pt.|ICVS/3B's-PT Government Associate Laboratory, Braga/Guimarães, Portugal. agostinhocarvalho@ecsaude.uminho.pt.
authors.forename: Agostinho
authors.initials: A
authors.lastname: Carvalho
mesh_terms: D015415:Biomarkers / Q000097:blood; D002097:C-Reactive Protein / Q000235:genetics / Q000378:metabolism; D002469:Cell Separation; D020022:Genetic Predisposition to Disease; D060005:Genotyping Techniques; D006801:Humans; D000072742:Invasive Fungal Infections / Q000175:diagnosis / Q000235:genetics* / Q000382:microbiology; D009504:Neutrophils / Q000378:metabolism; D020641:Polymorphism, Single Nucleotide; D018570:Risk Assessment; D000683:Serum Amyloid P-Component / Q000235:genetics / Q000378:metabolism; D000099153:Pentraxins
chemical_list: D015415:Biomarkers; D002097:C-Reactive Protein; D000683:Serum Amyloid P-Component; D000099153:Pentraxins
keywords: Antifungal immunity; Host biomarkers; Immunocompromised patients; Invasive fungal disease; Long pentraxin 3 (PTX3); Personalized medicine; Risk stratification; Single nucleotide polymorphism (SNP)