title: Biocompatible and fluorescent superparamagnetic iron oxide nanoparticles with superior magnetic properties coated with charged polysaccharide derivatives.
abstract: Syntheses and characterizations of biocompatible superparamagnetic iron oxide nanoparticles with embedded curcumin and coated with ultrathin layer of hyaluronic acid-curcumin (HA-Cur) conjugate have been reported. Zeta potential measurements confirmed effective coating of native iron oxide nanoparticles stabilized by cationic derivative of chitosan (SPION-CCh) with the synthesized HA-Cur conjugate. Both SPIONs with embedded curcumin and the ones coated with HA-Cur (SPION-CCh/HA-Cur) revealed desired magnetic characteristics while fluorescent properties were much better for the coated nanoparticles. SPION-CCh/HA-Cur nanoparticles were shown to be very promising candidates for T2MRI contrast agents as they can easily penetrate cell membrane and their relaxivity is exceptionally high (ca. 470mM-1s-1). They may be also tracked using confocal fluorescence microscopy due to the presence of fluorescent curcumin in the coating. In vitro studies indicated that the obtained SPIONs-CCh/HA-Cur were non-toxic for EA.hy926 endothelial cells.
authors.affiliation: Faculty of Chemistry, Jagiellonian University, Ingardena 3, 30-060 Krakow, Poland; Academic Centre of Materials and Nanotechnology, AGH - University of Science and Technology, Kawiory 30, 30-055 Krakow, Poland.
authors.forename: Dorota
authors.initials: D
authors.lastname: Lachowicz
authors.affiliation: Faculty of Chemistry, Jagiellonian University, Ingardena 3, 30-060 Krakow, Poland.
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Szpak
authors.affiliation: Research Centre for Nanometer-Scale Science and Advanced Materials, NANOSAM, Faculty of Physics, Astronomy and Applied Computer Science, Jagiellonian University, Łojasiewicza 11, 30-348 Krakow, Poland.
authors.forename: Katarzyna E
authors.initials: KE
authors.lastname: Malek-Zietek
authors.affiliation: Faculty of Chemistry, Jagiellonian University, Ingardena 3, 30-060 Krakow, Poland.
authors.forename: Mariusz
authors.initials: M
authors.lastname: Kepczynski
authors.affiliation: Department of General, Organic and Biomedical Chemistry, NMR and Molecular Imaging Laboratory, University of Mons, Avenue Maistriau, 19, B-7000 Mons, Belgium; Center for Microscopy and Molecular Imaging (CMMI), Rue A. Bolland, 8, 6041Gosselies, Belgium.
authors.forename: Robert N
authors.initials: RN
authors.lastname: Muller
authors.affiliation: Department of General, Organic and Biomedical Chemistry, NMR and Molecular Imaging Laboratory, University of Mons, Avenue Maistriau, 19, B-7000 Mons, Belgium; Center for Microscopy and Molecular Imaging (CMMI), Rue A. Bolland, 8, 6041Gosselies, Belgium.
authors.forename: Sophie
authors.initials: S
authors.lastname: Laurent
authors.affiliation: Faculty of Chemistry, Jagiellonian University, Ingardena 3, 30-060 Krakow, Poland. Electronic address: nowakows@chemia.uj.edu.pl.
authors.forename: Maria
authors.initials: M
authors.lastname: Nowakowska
authors.affiliation: Faculty of Chemistry, Jagiellonian University, Ingardena 3, 30-060 Krakow, Poland. Electronic address: s.zapotoczny@uj.edu.pl.
authors.forename: Szczepan
authors.initials: S
authors.lastname: Zapotoczny
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism; D003287:Contrast Media / Q000737:chemistry; D003474:Curcumin / Q000737:chemistry; D003911:Dextrans / Q000737:chemistry*; D042783:Endothelial Cells / Q000187:drug effects; D005290:Ferric Compounds / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry; D006801:Humans; D006820:Hyaluronic Acid / Q000737:chemistry; D008027:Light; D008279:Magnetic Resonance Imaging; D008280:Magnetics; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D051379:Mice; D041681:NIH 3T3 Cells; D011134:Polysaccharides / Q000737:chemistry*; D012542:Scattering, Radiation; D013050:Spectrometry, Fluorescence; D013053:Spectrophotometry
chemical_list: D001672:Biocompatible Materials; D003287:Contrast Media; D003911:Dextrans; D005290:Ferric Compounds; D005456:Fluorescent Dyes; D058185:Magnetite Nanoparticles; D011134:Polysaccharides; C000499:ferric oxide; D006820:Hyaluronic Acid; C065507:ferumoxides; D003474:Curcumin
keywords: Cationic chitosan; Curcumin; Hyaluronic acid; Nanoparticles; SPION