title: Transcript, protein and metabolite temporal dynamics in the CAM plant Agave.
abstract: Already a proven mechanism for drought resilience, crassulacean acid metabolism (CAM) is a specialized type of photosynthesis that maximizes water-use efficiency by means of an inverse (compared to C3and C4photosynthesis) day/night pattern of stomatal closure/opening to shift CO2uptake to the night, when evapotranspiration rates are low. A systems-level understanding of temporal molecular and metabolic controls is needed to define the cellular behaviour underpinning CAM. Here, we report high-resolution temporal behaviours of transcript, protein and metabolite abundances across a CAM diel cycle and, where applicable, compare the observations to the well-established C3model plant Arabidopsis. A mechanistic finding that emerged is that CAM operates with a diel redox poise that is shifted relative to that in Arabidopsis. Moreover, we identify widespread rescheduled expression of genes associated with signal transduction mechanisms that regulate stomatal opening/closing. Controlled production and degradation of transcripts and proteins represents a timing mechanism by which to regulate cellular function, yet knowledge of how this molecular timekeeping regulates CAM is unknown. Here, we provide new insights into complex post-transcriptional and -translational hierarchies that govern CAM in Agave. These data sets provide a resource to inform efforts to engineer more efficient CAM traits into economically valuable C3crops.
authors.affiliation: Chemical Sciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee 37831, USA.
authors.forename: Paul E
authors.initials: PE
authors.lastname: Abraham
authors.affiliation: Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee 37831, USA.
authors.forename: Hengfu
authors.identifier: 0000-0002-0720-5311
authors.initials: H
authors.lastname: Yin
authors.affiliation: Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee 37831, USA.|School of Biology, University of Newcastle, Newcastle upon Tyne NE1 7RU, UK.
authors.forename: Anne M
authors.initials: AM
authors.lastname: Borland
authors.affiliation: Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee 37831, USA.|The Bredesen Center for Interdisciplinary Research and Graduate Education, University of Tennessee, Knoxville, Tennessee 37996, USA.
authors.forename: Deborah
authors.identifier: 0000-0003-4979-5871
authors.initials: D
authors.lastname: Weighill
authors.affiliation: Department of Biochemistry and Molecular Biology, University of Nevada, MS330, Reno, Nevada 89557-0330, USA.
authors.forename: Sung Don
authors.initials: SD
authors.lastname: Lim
authors.affiliation: Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee 37831, USA.
authors.forename: Henrique Cestari
authors.identifier: 0000-0001-8494-0603
authors.initials: HC
authors.lastname: De Paoli
authors.affiliation: Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee 37831, USA.
authors.forename: Nancy
authors.initials: N
authors.lastname: Engle
authors.affiliation: Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee 37831, USA.|The Bredesen Center for Interdisciplinary Research and Graduate Education, University of Tennessee, Knoxville, Tennessee 37996, USA.
authors.forename: Piet C
authors.initials: PC
authors.lastname: Jones
authors.affiliation: Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee 37831, USA.
authors.forename: Ryan
authors.initials: R
authors.lastname: Agh
authors.affiliation: Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee 37831, USA.
authors.forename: David J
authors.initials: DJ
authors.lastname: Weston
authors.affiliation: Environmental Sciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee 37831, USA.
authors.forename: Stan D
authors.initials: SD
authors.lastname: Wullschleger
authors.affiliation: Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee 37831, USA.
authors.forename: Timothy
authors.identifier: 0000-0002-9540-6622
authors.initials: T
authors.lastname: Tschaplinski
authors.affiliation: Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee 37831, USA.|The Bredesen Center for Interdisciplinary Research and Graduate Education, University of Tennessee, Knoxville, Tennessee 37996, USA.
authors.forename: Daniel
authors.initials: D
authors.lastname: Jacobson
authors.affiliation: Department of Biochemistry and Molecular Biology, University of Nevada, MS330, Reno, Nevada 89557-0330, USA.
authors.forename: John C
authors.initials: JC
authors.lastname: Cushman
authors.affiliation: Chemical Sciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee 37831, USA.
authors.forename: Robert L
authors.initials: RL
authors.lastname: Hettich
authors.affiliation: Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee 37831, USA.
authors.forename: Gerald A
authors.initials: GA
authors.lastname: Tuskan
authors.affiliation: Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee 37831, USA.
authors.forename: Xiaohan
authors.identifier: 0000-0001-5207-4210
authors.initials: X
authors.lastname: Yang
mesh_terms: D027882:Agave / Q000235:genetics*; D002940:Circadian Rhythm; D003624:Darkness; D018506:Gene Expression Regulation, Plant; D053263:Gene Regulatory Networks; D008027:Light; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism
chemical_list: D010940:Plant Proteins