title: Fluorescent rare earth solutions as intrinsic wavelength standards for protein fluorescence spectroscopy.
abstract: Trivalent Gd, Tm, and Dy solutions can be used as intrinsic excitation and emission standards to validate the UV and violet-blue wavelength accuracy of a spectrofluorimeter. Europium extends the range into the red. To attain sufficient sensitivity, these luminescent rare earth ions require deuterated reagents or carbonate complexation, which allow the use of ordinary water and thus preparation in virtually any laboratory. Such solutions are particularly valuable as system suitability standards (SST) for protein fluorescence spectroscopy to detect red shifts of the intrinsic fluorescence maximum in stability and storage studies.
authors.affiliation: Shire Pharmaceuticals, Process Development & Technical Services/Analytical Development, Benatzkygasse 2-6, A-1220 Wien, Vienna, Austria.
authors.forename: Heinz
authors.initials: H
authors.lastname: Anderle
authors.affiliation: Shire Pharmaceuticals, Process Development & Technical Services/Analytical Development, Benatzkygasse 2-6, A-1220 Wien, Vienna, Austria. Electronic address: alfred.weber@shire.com.
authors.forename: Alfred
authors.initials: A
authors.lastname: Weber
mesh_terms: D002138:Calibration; D008674:Metals, Rare Earth / Q000737:chemistry*; D011506:Proteins / Q000032:analysis*; D013050:Spectrometry, Fluorescence / Q000379:methods / Q000592:standards
chemical_list: D008674:Metals, Rare Earth; D011506:Proteins
keywords: Dysprosium; Gadolinium; Instrument validation; Spectrofluorimeter; Thulium