title: IFN-τ Plays an Anti-Inflammatory Role in Staphylococcus aureus-Induced Endometritis in Mice Through the Suppression of NF-κB Pathway and MMP9 Expression.
abstract: Interferon-tau (IFN-τ) is a type I interferon and considered as a pregnancy recognition signal in ruminants. Our previous reports have confirmed that IFN-τ has a potential anti-inflammatory effect in macrophage. However, the anti-inflammatory effect of IFN-τ on endometritis has never been reported. Thus, the aim of this study was to investigate the effects of IFN-τ in a mouse model of Staphylococcus aureus-induced endometritis. The histopathological and myeloperoxidase activity results showed that IFN-τ could protect the uterus from S. aureus damage. Enzyme-linked immunosorbent assay and quantitative real-time polymerase chain reaction results revealed that IFN-τ inhibited TNF-α, IL-1β, and IL-6 production. TLR2, involved in the S. aureus infection, was downregulated by IFN-τ and directly activated nuclear transcription factor kappa-B (NF-κB) pathway. Then, we measured the phosphorylation of IκBα and NF-κB p65 by Western blotting. Western blotting results indicated that IFN-τ inhibited the phosphorylation of IκBα and NF-κB p65 in the S. aureus-induced endometritis. Matrix metalloproteinase (MMP)9, which has been reported to be regulated by NF-κB, was also suppressed by IFN-τ, but its inhibitors, tissue inhibitor of metalloproteinases1 level, increased. All of these findings suggested that IFN-τ plays an anti-inflammatory role in S. aureus-induced endometritis by suppressing NF-κB pathway and MMP9 expression.
authors.affiliation: 1 Department of Clinical Veterinary Medicine, College of Veterinary Medicine, Huazhong Agricultural University , Wuhan, People's Republic of China .
authors.forename: Kangfeng
authors.initials: K
authors.lastname: Jiang
authors.affiliation: 1 Department of Clinical Veterinary Medicine, College of Veterinary Medicine, Huazhong Agricultural University , Wuhan, People's Republic of China .
authors.forename: Xiuying
authors.initials: X
authors.lastname: Chen
authors.affiliation: 1 Department of Clinical Veterinary Medicine, College of Veterinary Medicine, Huazhong Agricultural University , Wuhan, People's Republic of China .
authors.forename: Gan
authors.initials: G
authors.lastname: Zhao
authors.affiliation: 1 Department of Clinical Veterinary Medicine, College of Veterinary Medicine, Huazhong Agricultural University , Wuhan, People's Republic of China .
authors.forename: Haichong
authors.initials: H
authors.lastname: Wu
authors.affiliation: 1 Department of Clinical Veterinary Medicine, College of Veterinary Medicine, Huazhong Agricultural University , Wuhan, People's Republic of China .
authors.forename: Junxian
authors.initials: J
authors.lastname: Mi
authors.affiliation: 1 Department of Clinical Veterinary Medicine, College of Veterinary Medicine, Huazhong Agricultural University , Wuhan, People's Republic of China .
authors.forename: Changwei
authors.initials: C
authors.lastname: Qiu
authors.affiliation: 2 Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction, Ministry of Education, Huazhong Agricultural University , Wuhan, People's Republic of China .
authors.forename: Xiuli
authors.initials: X
authors.lastname: Peng
authors.affiliation: 1 Department of Clinical Veterinary Medicine, College of Veterinary Medicine, Huazhong Agricultural University , Wuhan, People's Republic of China .
authors.forename: Ganzhen
authors.initials: G
authors.lastname: Deng
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D016207:Cytokines / Q000378:metabolism; D004195:Disease Models, Animal; D004716:Endometritis / Q000188:drug therapy / Q000378:metabolism* / Q000382:microbiology* / Q000473:pathology; D004789:Enzyme Activation; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects; D018836:Inflammation Mediators / Q000378:metabolism; D007370:Interferon Type I / Q000494:pharmacology*; D020780:Matrix Metalloproteinase 9 / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D016328:NF-kappa B / Q000378:metabolism*; D009195:Peroxidase / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects*; D013211:Staphylococcus aureus / Q000187:drug effects*; D051195:Toll-Like Receptor 2 / Q000235:genetics / Q000378:metabolism
chemical_list: D000893:Anti-Inflammatory Agents; D016207:Cytokines; D018836:Inflammation Mediators; D007370:Interferon Type I; D016328:NF-kappa B; D051195:Toll-Like Receptor 2; D009195:Peroxidase; D020780:Matrix Metalloproteinase 9
keywords: IFN-τ; MMP9; NF-κB; S. aureus; endometritis