title: Cell surface hydrophobicity and colony morphology of Trichosporon asahii clinical isolates.
abstract: Trichosporon asahii is a pathogenic basidiomycetous yeast. Individual strains of T. asahii have different colony morphologies. However, it is not clear whether cell surface phenotypes differ among the colony morphologies. Here we characterized the cell surface hydrophobicity and analysed the carbohydrate contents of the cell surface polysaccharides in T. asahii clinical isolates with various colony morphologies. Among the three distinctive colony morphologies obtained from one clinical isolate, the white-type morphology exhibited higher hydrophobicity. The hydrophobicity of heat-killed T. asahii cells was greatly reduced after periodate oxidation of the cell surface carbohydrates. Furthermore, the cell wall and extracellular polysaccharide components differed among the morphologies. Our results suggest that T. asahii cell surface hydrophobicity is affected by cell surface carbohydrate composition. Copyright © 2016 John Wiley & Sons, Ltd.
authors.affiliation: Department of Microbial Science and Host Defense, Meiji Pharmaceutical University, 2-522-1, Noshio, Kiyose, Tokyo, 204-8588, Japan.
authors.forename: Tomoe
authors.initials: T
authors.lastname: Ichikawa
authors.affiliation: Department of Microbial Science and Host Defense, Meiji Pharmaceutical University, 2-522-1, Noshio, Kiyose, Tokyo, 204-8588, Japan.
authors.forename: Chihiro
authors.initials: C
authors.lastname: Hirata
authors.affiliation: Department of Microbial Science and Host Defense, Meiji Pharmaceutical University, 2-522-1, Noshio, Kiyose, Tokyo, 204-8588, Japan.
authors.forename: Mizuki
authors.initials: M
authors.lastname: Takei
authors.affiliation: Department of Microbial Science and Host Defense, Meiji Pharmaceutical University, 2-522-1, Noshio, Kiyose, Tokyo, 204-8588, Japan.
authors.forename: Naoyuki
authors.initials: N
authors.lastname: Tagami
authors.affiliation: Department of Microbial Science and Host Defense, Meiji Pharmaceutical University, 2-522-1, Noshio, Kiyose, Tokyo, 204-8588, Japan.
authors.forename: Hiromi
authors.initials: H
authors.lastname: Murasawa
authors.affiliation: Department of Microbial Science and Host Defense, Meiji Pharmaceutical University, 2-522-1, Noshio, Kiyose, Tokyo, 204-8588, Japan.
authors.forename: Reiko
authors.initials: R
authors.lastname: Ikeda
mesh_terms: D018441:Biofilms; D002241:Carbohydrates / Q000032:analysis; D002448:Cell Adhesion; D002462:Cell Membrane / Q000737:chemistry / Q000502:physiology; D057927:Hydrophobic and Hydrophilic Interactions; D011134:Polysaccharides / Q000032:analysis; D014250:Trichosporon / Q000737:chemistry / Q000502:physiology*
chemical_list: D002241:Carbohydrates; D011134:Polysaccharides
keywords: carbohydrates; cell surface hydrophobicity; cell wall; yeast