title: Disruption of diphenylalanine assembly by a Boc-modified variant.
abstract: Peptide-based biomaterials are key to the future of diagnostics and therapy, promoting applications such as tissue scaffolds and drug delivery vehicles. To realise the full potential of the peptide systems, control and optimisation of material properties are essential. Here we investigated the co-assembly of the minimal amyloid motif peptide, diphenylalanine (FF), and its tert-butoxycarbonyl (Boc)-modified derivative. Using Atomic Force Microscopy, we demonstrated that the co-assembled fibers are less rigid and show a curvier morphology. We propose that the Boc-modification of FF disrupts the hydrogen bond packing of adjacent N-termini, as supported by Fourier transform infrared and fluorescence spectroscopic data. Such rationally modified co-assemblies offer chemical functionality for after-assembly modification and controllable surface properties for tissue engineering scaffolds, along with tunable morphological vs. mechanical properties.
authors.affiliation: School of Pharmacy, University of Nottingham, NG7 2RD Nottingham, UK.
authors.forename: Rhiannon C G
authors.identifier: http://orcid.org/0000-0003-4780-4623
authors.initials: RC
authors.lastname: Creasey
authors.affiliation: School of Pharmacy, University of Nottingham, NG7 2RD Nottingham, UK.
authors.forename: Iria
authors.initials: I
authors.lastname: Louzao
authors.affiliation: Department for Molecular Microbiology and Biotechnology, Tel Aviv University, 6997801 Tel Aviv, Israel.
authors.forename: Zohar A
authors.initials: ZA
authors.lastname: Arnon
authors.affiliation: Department for Molecular Microbiology and Biotechnology, Tel Aviv University, 6997801 Tel Aviv, Israel.
authors.forename: Pini
authors.initials: P
authors.lastname: Marco
authors.affiliation: Department of Oral Biology, The Goldschleger School of Dental Medicine, Sackler Faculty of Medicine, Tel Aviv University, 6997801 Tel Aviv, Israel.
authors.forename: Lihi
authors.initials: L
authors.lastname: Adler-Abramovich
authors.affiliation: School of Pharmacy, University of Nottingham, NG7 2RD Nottingham, UK.
authors.forename: Clive J
authors.initials: CJ
authors.lastname: Roberts
authors.affiliation: Department for Molecular Microbiology and Biotechnology, Tel Aviv University, 6997801 Tel Aviv, Israel.
authors.forename: Ehud
authors.initials: E
authors.lastname: Gazit
authors.affiliation: School of Pharmacy, University of Nottingham, NG7 2RD Nottingham, UK.
authors.forename: Saul J B
authors.initials: SJ
authors.lastname: Tendler