title: [The effect of Foxc2 overexpression on the osteogenic properties of C3H10T1/2 cells].
abstract: OBJECTIVE
  To investigate the effect of Foxc2 overexpression on osteogenic and adipogenic differentiation of C3H10T1/2 cells.
  
  
  METHODS
  C3H10T1/2 cells were transfected with plenti-Foxc2 and selected with puromycin for stable clones. The expression of Foxc2 was determined by real-time PCR and Western blot. Cell proliferation was detected by CCK-8 kit. Cell cycle and apoptosis were detected by flow cytometry. The level of osteogenic biomarkers Runx2, OPN, OCN and adipogenic biomarker PPARγ were quantified by real-time PCR and Western blot. Alkaline phosphatase (ALP) staining and oil red staining were conducted to evaluate the effect of Foxc2 overexpression on osteogenic and adipogenic differentiation. Statistical analysis was performed using SPSS 17.0 software package.
  
  
  RESULTS
  C3H10T1/2-Foxc2 cell line was successfully constructed and verified by direct sequencing and Foxc2 overexpression in vitro. Cell proliferation was reduced and cell cycle was blocked in G1/G0 phase. Enhanced ALP staining and reduced oil red staining were observed in C3H10T1/2-Foxc2 cells as compared with the control. Foxc2 overexpression up-regulated Runx2, OPN, OCN during osteogenic differentiation and down-regulated PPARγduring adipogenic differentiation.
  
  
  CONCLUSIONS
  C3H10T1/2 cell line stably expressing Foxc2 gene was successfully established, cell proliferation was reduced, osteogenesis biomarkers were up-regulated during the osteogenesis by overexpression Foxc2, PPARγwas down-regulated during adipogenesis.
authors.affiliation: Department of Oral and Craniomaxillofacial Science, Shanghai Ninth People's Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine; Shanghai Key Laboratory of Stomatology. Shanghai 200011, China. E-mail:wangminjiao442773955@126.com.
authors.forename: Min-Jiao
authors.initials: MJ
authors.lastname: Wang
authors.forename: Jia-Wen
authors.initials: JW
authors.lastname: Si
authors.forename: Hong-Liang
authors.initials: HL
authors.lastname: Li
authors.forename: Ning-Juan
authors.initials: NJ
authors.lastname: Ouyang
authors.forename: Guo-Fang
authors.initials: GF
authors.lastname: Shen
mesh_terms: D001854:Bone Marrow Cells; D002454:Cell Differentiation; D002460:Cell Line; D002478:Cells, Cultured; D051858:Forkhead Transcription Factors; D006801:Humans; D059630:Mesenchymal Stem Cells; D010012:Osteogenesis; D047495:PPAR gamma; D060888:Real-Time Polymerase Chain Reaction; D014162:Transfection
chemical_list: D051858:Forkhead Transcription Factors; D047495:PPAR gamma; C082078:mesenchyme fork head 1 protein