title: Antioxidant properties of tea blunt ROS-dependent lipogenesis: beneficial effect on hepatic steatosis in a high fat-high sucrose diet NAFLD obese rat model.
abstract: Oxidative stress could trigger lipid accumulation in liver and thus hepatic steatosis. Tea is able to prevent liver disorders, but a direct link between antioxidant capacities and prevention of steatosis has not been reported yet. We aimed to investigate such relationship in a rat model of high fat-high sucrose diet (HFS)-induced obesity and to explore more deeply the mechanisms in isolated hepatocytes. Wistar rats were divided into a control group (standard diet), an HFS group (high fat-sucrose diet) and an HFS+tea group (HFS diet with ad-libitum access to tea drink). Body weight, fat mass, glycemic parameters in blood, lipid and oxidative stress parameters in blood and liver were measured in each group after 14 weeks. Isolated hepatocytes were treated with the reactive oxygen species (ROS) inducer t-BHP in the presence or not of antioxidants (tempol or tea), and superoxide anion production and lipid accumulation were measured using specific fluorescent probes. We reported that the HFS diet highly increased hepatic lipids content, while tea consumption attenuated steatosis and improved the oxidative status (decrease in hepatic oxidative stress, increase in plasma total antioxidant capacity). The role of antioxidant properties of tea in such phenomenon was confirmed in primary cultured rat hepatocytes. Indeed, the increase of mitochondrial ROS production with t-BHP resulted in lipid accumulation in hepatocytes (positive linear regression), and antioxidants (tempol or tea) normalized both. We reported that the antioxidant properties of tea protect rats from an obesogenic HFS diet-induced hepatic steatosis by counteracting the ROS-dependent lipogenesis.
authors.affiliation: Laboratoire Protee, Equipe EB2M, Université de Toulon, avenue de l'Université, 83130 La Garde, France. Electronic address: lbraud01@gmail.com.
authors.forename: Laura
authors.initials: L
authors.lastname: Braud
authors.affiliation: Laboratoire de Pharm-Ecologie Cardiovasculaire (EA4278), Faculty of Sciences, Avignon University, 33 rue Louis Pasteur, 84000 Avignon, France.
authors.forename: Sylvain
authors.initials: S
authors.lastname: Battault
authors.affiliation: Laboratoire de Pharm-Ecologie Cardiovasculaire (EA4278), Faculty of Sciences, Avignon University, 33 rue Louis Pasteur, 84000 Avignon, France.
authors.forename: Grégory
authors.initials: G
authors.lastname: Meyer
authors.affiliation: Laboratoire de Pharm-Ecologie Cardiovasculaire (EA4278), Faculty of Sciences, Avignon University, 33 rue Louis Pasteur, 84000 Avignon, France.
authors.forename: Alessandro
authors.initials: A
authors.lastname: Nascimento
authors.affiliation: Plateforme BioTechServices, Université de Toulon, avenue de l'Université, 83130 La Garde, France.
authors.forename: Sandrine
authors.initials: S
authors.lastname: Gaillard
authors.affiliation: UMR 1331 TOXALIM (Research Centre in Food Toxicology), Institut National de la Recherche Agronomique (INRA), Laboratory of Xenobiotic's Cellular and Molecular Toxicology, 400 route des Chappes, 06903 Sophia-Antipolis, France.
authors.forename: Georges
authors.initials: G
authors.lastname: de Sousa
authors.affiliation: UMR 1331 TOXALIM (Research Centre in Food Toxicology), Institut National de la Recherche Agronomique (INRA), Laboratory of Xenobiotic's Cellular and Molecular Toxicology, 400 route des Chappes, 06903 Sophia-Antipolis, France.
authors.forename: Roger
authors.initials: R
authors.lastname: Rahmani
authors.affiliation: Laboratoire de Pharm-Ecologie Cardiovasculaire (EA4278), Faculty of Sciences, Avignon University, 33 rue Louis Pasteur, 84000 Avignon, France.
authors.forename: Catherine
authors.initials: C
authors.lastname: Riva
authors.affiliation: Aix-Marseille Université, CNRS, CRMBM UMR 7339, F-13385 Marseille, France.
authors.forename: Martine
authors.initials: M
authors.lastname: Armand
authors.affiliation: Laboratoire Protee, Equipe EB2M, Université de Toulon, avenue de l'Université, 83130 La Garde, France.
authors.forename: Jean-Michel
authors.initials: JM
authors.lastname: Maixent
authors.affiliation: Laboratoire de Pharm-Ecologie Cardiovasculaire (EA4278), Faculty of Sciences, Avignon University, 33 rue Louis Pasteur, 84000 Avignon, France.
authors.forename: Cyril
authors.initials: C
authors.lastname: Reboul
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism / Q000494:pharmacology*; D002478:Cells, Cultured; D059305:Diet, High-Fat / Q000009:adverse effects*; D004195:Disease Models, Animal; D022781:Hepatocytes / Q000378:metabolism; D015227:Lipid Peroxidation; D050155:Lipogenesis / Q000502:physiology*; D008297:Male; D065626:Non-alcoholic Fatty Liver Disease / Q000178:diet therapy* / Q000209:etiology; D009765:Obesity / Q000178:diet therapy / Q000503:physiopathology; D018384:Oxidative Stress; D017208:Rats, Wistar; D017382:Reactive Oxygen Species / Q000378:metabolism; D013662:Tea / Q000737:chemistry
chemical_list: D000975:Antioxidants; D017382:Reactive Oxygen Species; D013662:Tea
keywords: Antilipogenesis; Antioxidant; Camellia sinensis; Mitochondrial ROS; NAFLD; Obesogenic diet