title: Follistatin-like protein 1 contributes to dendritic cell and T-lymphocyte activation in nasopharyngeal carcinoma patients by altering nuclear factor κb and Jun N-terminal kinase expression.
abstract: Follistatin-like protein 1 (FSTL1) is a newly characterized protein that can regulate the immune response in various ways. Dendritic cells (DCs) are central to immune regulation. In this study, we explored the impact of FSTL1 on DC activity in nasopharyngeal carcinoma (NPC) patients. The surface expression of CD40, CD86, and HLA-DR on DCs was analyzed and showed significantly elevated expression levels, indicating DC maturity. After FSTL1 was added to DCs collected from NPC patients (n = 50), controls (n = 47), and healthy donors (n = 10), interferon γ secretion and T-cell receptor expression in cytotoxic T lymphocytes were also investigated. In the experimental groups, the expression of the critical immune protein nuclear factor (NF)-κb was upregulated, whereas Jun N-terminal kinase (JNK) was downregulated. Our findings demonstrate that FSTL1 plays a critical role in immune regulation, enhancing the antigen presentation ability of DCs by up-regulating NF-κb expression and down-regulating JNK expression.
authors.affiliation: Department of Otorhinolaryngology Head and Neck Surgery, First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Wang
authors.affiliation: Department of Otorhinolaryngology Head and Neck Surgery, The People's Hospital, Guigang, Guangxi, China.
authors.forename: Senyong
authors.initials: S
authors.lastname: Wu
authors.affiliation: Department of Otorhinolaryngology Head and Neck Surgery, The People's Hospital, Guigang, Guangxi, China.
authors.forename: Shiping
authors.initials: S
authors.lastname: Huang
authors.affiliation: Department of Otorhinolaryngology Head and Neck Surgery, The People's Hospital, Guigang, Guangxi, China.|Department of Otolaryngology, The Cooperation of Chinese and Western Medicine Hospital in Guangzhou, Guangzhou, China.
authors.forename: Shaolin
authors.initials: S
authors.lastname: Yin
authors.affiliation: Department of Otorhinolaryngology Head and Neck Surgery, The People's Hospital, Guigang, Guangxi, China.|Department of Otolaryngology, The People's Hospital of Hezhou, Guangxi, China.
authors.forename: Guilong
authors.initials: G
authors.lastname: Zou
authors.affiliation: Department of Otorhinolaryngology Head and Neck Surgery, First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi, China.|Department of Otorhinolaryngology Head and Neck Surgery, The People's Hospital, Guigang, Guangxi, China.
authors.forename: Kuan'en
authors.initials: K
authors.lastname: Huang
authors.affiliation: Department of Otorhinolaryngology Head and Neck Surgery, First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi, China.|Department of Otolaryngology, The Cooperation of Chinese and Western Medicine Hospital in Guangzhou, Guangzhou, China.
authors.forename: Zhe
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: Department of Otorhinolaryngology Head and Neck Surgery, First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi, China.
authors.forename: Anzhou
authors.initials: A
authors.lastname: Tang
authors.affiliation: Department of Otorhinolaryngology Head and Neck Surgery, First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi, China.
authors.forename: Wensheng
authors.initials: W
authors.lastname: Wen
mesh_terms: D000328:Adult; D015153:Blotting, Western; D002277:Carcinoma; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000378:metabolism*; D005260:Female; D038702:Follistatin-Related Proteins / Q000378:metabolism*; D006801:Humans; D007371:Interferon-gamma / Q000096:biosynthesis; D048031:JNK Mitogen-Activated Protein Kinases / Q000378:metabolism*; D008213:Lymphocyte Activation; D018655:Lymphocyte Count; D008297:Male; D008875:Middle Aged; D016328:NF-kappa B / Q000378:metabolism*; D000077274:Nasopharyngeal Carcinoma; D009303:Nasopharyngeal Neoplasms / Q000276:immunology*; D060888:Real-Time Polymerase Chain Reaction; D011948:Receptors, Antigen, T-Cell / Q000378:metabolism; D013601:T-Lymphocytes / Q000378:metabolism*
chemical_list: D038702:Follistatin-Related Proteins; D016328:NF-kappa B; D011948:Receptors, Antigen, T-Cell; C448799:FSTL1 protein, human; D007371:Interferon-gamma; D048031:JNK Mitogen-Activated Protein Kinases
keywords: Jun N-terminal kinase; T lymphocyte; dendritic cell; follistatin-like protein 1; nasopharyngeal carcinoma; nuclear factor κb