title: Novel electrochemical biosensor based on PVP capped CoFe2O4@CdSe core-shell nanoparticles modified electrode for ultra-trace level determination of rifampicin by square wave adsorptive stripping voltammetry.
abstract: This work introduces a new electrochemical sensor based on polyvinyl pyrrolidone capped CoFe2O4@CdSe core-shell modified electrode for a rapid detection and highly sensitive determination of rifampicin (RIF) by square wave adsorptive stripping voltammetry. The new PVP capped CoFe2O4@CdSe with core-shell nanostructure was synthesized by a facile synthesis method for the first time. PVP can act as a capping and etching agent for protection of the outer surface nanoparticles and formation of a mesoporous shell, respectively. Another important feature of this work is the choice of the ligand (1,10-phenanthroline) for precursor cadmium complex that works as a chelating agent in order to increase optical and electrical properties and stability of prepared nanomaterial. The nanoparticles have been characterized by field emission scanning electron microscopy (FESEM), transmission electron microscope (TEM), energy dispersive X-ray spectroscopy (EDX), X-ray diffraction (XRD), UV-vis, photoluminescence (PL) spectroscopy, FT-IR, and cyclic voltammetry techniques. The PL spectroscopy study of CoFe2O4@CdSe has shown significant PL quenching by the formation of CoFe2O4core inside CdSe, this shows that CoFe2O4NPs are efficient electron acceptors with the CdSe. It is clearly observed that the biosensor can significantly enhance electrocatalytic activity towards the oxidation of RIF, under the optimal conditions. The novelty of this work arises from the new synthesis method for the core-shell of CoFe2O4@CdSe. Then, the novel electrochemical biosensor was fabricated for ultra-trace level determination of rifampicin with very low detection limit (4.55×10-17M) and a wide linear range from 1.0×10-16to 1.0×10-7M. The fabricated biosensor showed high sensitivity and selectivity, good reproducibility and stability. Therefore, it was successfully applied for the determination of ultra-trace RIF amounts in biological and pharmaceutical samples with satisfactory recovery data.
authors.affiliation: Department of Analytical Chemistry, Faculty of Chemistry, University of Tabriz, Tabriz 51666-16471, Iran. Electronic address: asadpour@tabrizu.ac.ir.
authors.forename: Karim
authors.initials: K
authors.lastname: Asadpour-Zeynali
authors.affiliation: Department of Analytical Chemistry, Faculty of Chemistry, University of Tabriz, Tabriz 51666-16471, Iran.
authors.forename: Fariba
authors.initials: F
authors.lastname: Mollarasouli
mesh_terms: D000904:Antibiotics, Antitubercular / Q000032:analysis / Q000097:blood*; D015374:Biosensing Techniques / Q000295:instrumentation; D019187:Cadmium Compounds / Q000737:chemistry*; D003035:Cobalt / Q000737:chemistry*; D055664:Electrochemical Techniques / Q000295:instrumentation*; D005290:Ferric Compounds / Q000737:chemistry*; D006801:Humans; D007917:Leprostatic Agents / Q000032:analysis / Q000097:blood; D057230:Limit of Detection; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D011205:Povidone / Q000737:chemistry*; D015203:Reproducibility of Results; D012293:Rifampin / Q000032:analysis / Q000097:blood*; D018036:Selenium Compounds / Q000737:chemistry*; D013607:Tablets
chemical_list: D000904:Antibiotics, Antitubercular; D019187:Cadmium Compounds; D005290:Ferric Compounds; D007917:Leprostatic Agents; D018036:Selenium Compounds; D013607:Tablets; C569492:cobalt ferrite; D003035:Cobalt; C058667:cadmium selenide; D011205:Povidone; D012293:Rifampin
keywords: Electrochemical biosensor; Glassy carbon electrode; PVP capped CoFe(2)O(4)@CdSe magnetic nanoparticles; Rifampicin; Square wave adsorptive stripping voltammetry (SWASV)