title: Advancement of green process through microwave-assisted extraction of bioactive metabolites from Arthrospira Platensis and bioactivity evaluation.
abstract: Bioactivity and functional properties of cyanobacterial extract mostly depends on process of extraction, temperature and solvent used (polar or non-polar). To evaluate these parameters a design of experiment (DOE; using a 2kdesign) was performed with Arthrospira platensis. Extraction process was optimized through microwave-assisted extraction considering solvent ratio, temperature and time of extraction with polar (PS) and non-polar (NPS). Maximum extract yield obtained was 4.32±0.25% and 5.26±0.11% (w/w) respectively for PS and NPS. Maximum content of bioactive metabolites in PS extracts were thiamine (846.57±14.12μg/g), riboflavin (101.09±1.63μg/g), C-phycocyanin (2.28±0.10μg/g) and A-phycocyanin (4.11±0.03μg/g), while for NPS extracts were α-tocopherol (37.86±0.78μg/g), β-carotene (123.64±1.45μg/g) and 19.44±0.21mg/g of fatty acids. A. platensis PS extracts showed high antimicrobial activity and PS extracts had antioxidant activity of 0.79±0.12μmolTE/g for FRAP assay, while for NPS extracts 1.03±0.08μmol α-TE/g for FRAP assay.
authors.affiliation: Tecnologico de Monterrey, Escuela Nacional de Ciencias, Ingenieria y Tecnologia, Campus Monterrey, Ave. Eugenio Garza Sada 2501, Monterrey, N.L. 64849, Mexico.
authors.forename: Diego A
authors.initials: DA
authors.lastname: Esquivel-Hernández
authors.affiliation: Tecnologico de Monterrey, Escuela Nacional de Ciencias, Ingenieria y Tecnologia, Campus Monterrey, Ave. Eugenio Garza Sada 2501, Monterrey, N.L. 64849, Mexico.
authors.forename: José
authors.initials: J
authors.lastname: Rodríguez-Rodríguez
authors.affiliation: Tecnologico de Monterrey, Escuela Nacional de Ciencias, Ingenieria y Tecnologia, Campus Monterrey, Ave. Eugenio Garza Sada 2501, Monterrey, N.L. 64849, Mexico.
authors.forename: Magdalena
authors.initials: M
authors.lastname: Rostro-Alanis
authors.affiliation: Laboratory of Aquatic Biology, KU Leuven Kulak, E. Sabbelaan 53, 8500 Kortrijk, Belgium.
authors.forename: Sara P
authors.initials: SP
authors.lastname: Cuéllar-Bermúdez
authors.affiliation: Tecnologico de Monterrey, Escuela Nacional de Ciencias, Ingenieria y Tecnologia, Campus Monterrey, Ave. Eugenio Garza Sada 2501, Monterrey, N.L. 64849, Mexico.
authors.forename: Elena I
authors.initials: EI
authors.lastname: Mancera-Andrade
authors.affiliation: Tecnologia Ambiental Biomex S.A. de C.V., Volcan Jorullo 5268, Zapopan, Jalisco 45070, Mexico.
authors.forename: Jade E
authors.initials: JE
authors.lastname: Núñez-Echevarría
authors.affiliation: Tecnologico de Monterrey, Escuela Nacional de Ciencias, Ingenieria y Tecnologia, Campus Monterrey, Ave. Eugenio Garza Sada 2501, Monterrey, N.L. 64849, Mexico.
authors.forename: J Saúl
authors.initials: JS
authors.lastname: García-Pérez
authors.affiliation: Tecnologico de Monterrey, Escuela Nacional de Ciencias, Ingenieria y Tecnologia, Campus Monterrey, Ave. Eugenio Garza Sada 2501, Monterrey, N.L. 64849, Mexico.
authors.forename: Rashmi
authors.initials: R
authors.lastname: Chandra
authors.affiliation: Tecnologico de Monterrey, Escuela Nacional de Ciencias, Ingenieria y Tecnologia, Campus Monterrey, Ave. Eugenio Garza Sada 2501, Monterrey, N.L. 64849, Mexico. Electronic address: r.parra@itesm.mx.
authors.forename: Roberto
authors.initials: R
authors.lastname: Parra-Saldívar
mesh_terms: D000890:Anti-Infective Agents / Q000302:isolation & purification / Q000494:pharmacology; D005591:Chemical Fractionation / Q000379:methods*; D008826:Microbial Sensitivity Tests; D008872:Microwaves; D010636:Phenols / Q000302:isolation & purification; D010798:Phycocyanin / Q000302:isolation & purification; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification; D012997:Solvents / Q000737:chemistry; D053545:Spirulina / Q000737:chemistry* / Q000254:growth & development / Q000378:metabolism; D019207:beta Carotene / Q000302:isolation & purification
chemical_list: D000890:Anti-Infective Agents; D010636:Phenols; D010936:Plant Extracts; D012997:Solvents; D019207:beta Carotene; D010798:Phycocyanin
keywords: FRAP; Microwave-assisted extraction; Phycocyanin; α-Tocopherol; β-Carotene; γ-Linolenic acid