title: Mercury detection based on label-free and isothermal enzyme-free amplified fluorescence platform.
abstract: A novel and convenient biosensor for Mercury (II) detection was developed based on toehold-mediated strand displacement isothermal enzyme-free amplification (EFA) technology and label-free fluorescence platform using Sybr Green Ι (SG) and graphene oxide (GO). The method is highly sensitive and selective, and the logarithmically related Hg2+linearity range is from 0.1 to 50nM with a detection limit 0.091nM. Moreover, our strategy is simple, enzyme-free, and inexpensive and can be applied to detect spiked Hg2+in environmental water samples with good recovery and accuracy, which demonstrates that the biosensor has a good potential in the environment surveys in the future.
authors.affiliation: School of Chemistry and Materials Science, Ludong University, Yantai 264025, China.
authors.forename: Fanbin
authors.initials: F
authors.lastname: Meng
authors.affiliation: School of Chemistry and Materials Science, Ludong University, Yantai 264025, China. Electronic address: xuhui235@163.com.
authors.forename: Hui
authors.initials: H
authors.lastname: Xu
authors.affiliation: School of Chemistry and Materials Science, Ludong University, Yantai 264025, China.
authors.forename: Xue
authors.initials: X
authors.lastname: Yao
authors.affiliation: School of Materials Science and Engineering, Lanzhou University of Technology, Lanzhou 730050, China.
authors.forename: Xuan
authors.initials: X
authors.lastname: Qin
authors.affiliation: School of Life Sciences, Ludong University, Yantai 264025, China.
authors.forename: Tingting
authors.initials: T
authors.lastname: Jiang
authors.affiliation: School of Chemistry and Materials Science, Ludong University, Yantai 264025, China.
authors.forename: Shanmin
authors.initials: S
authors.lastname: Gao
authors.affiliation: School of Chemistry and Materials Science, Ludong University, Yantai 264025, China.
authors.forename: Yahui
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: School of Chemistry and Materials Science, Ludong University, Yantai 264025, China.
authors.forename: Di
authors.initials: D
authors.lastname: Yang
authors.affiliation: School of Chemistry and Materials Science, Ludong University, Yantai 264025, China.
authors.forename: Xia
authors.initials: X
authors.lastname: Liu
keywords: Hg(2+); Isothermal enzyme-free amplification; Label-free fluorescence platform