title: Physicochemical and functional properties of gelatin extracted from Yak skin.
abstract: Different molecular weight distribution (MWD) gelatin was extracted from Yak skin after enzymatic pretreatments and their physicochemical and functional properties (SDS-PAGE, UV-vis absorption spectra, DSC, FT-IR, Amino acid analysis, AFM, emulsibility and foamability) were analyzed. The gelatin was extracted by pepsin and got different MWD of Yak skin gelatin by controlling the enzymolysis time. The SDS-PAGE showed the MWD of the Yak skin gelatin. The UV-vis absorption turned out that the broad MWD of Yak skin gelatin had a higher maximum absorption peaks. The FT-IR and AFM indicated that the gelatin structures and microstructures changed with the change of the MWD. The broad MWD of the Yak skin gelatin had a higher denaturation temperature (TD), and it was higher than most of the other mammals and marine biological gelatin. The broad MWD gelatin also had higher imino acids (proline and hydroxyproline) contents and lower foamability and emulsibility compared to the narrow MWD gelatin. These findings, obtained for the first time for Yak skin gelatin, showed that it has great potential for application as an alternative to commercial gelatin due to its good thermotolerance, particularly in the applications of the biological materials, stabilizer of thermo-tolerant and so on.
authors.affiliation: Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Qinghai key laboratory Of Tibetan Medicine Pharmacology and Safety Evaluation, 810008 Xining, China; Key Laboratory of Tibetan Medicine Research, Chinese Academy of Sciences, 810008 Xining, China; University of Chinese Academic of Sciences, 100049 Beijing, China.
authors.forename: Mengqi
authors.initials: M
authors.lastname: Xu
authors.affiliation: Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Qinghai key laboratory Of Tibetan Medicine Pharmacology and Safety Evaluation, 810008 Xining, China; Key Laboratory of Tibetan Medicine Research, Chinese Academy of Sciences, 810008 Xining, China.
authors.forename: Lixin
authors.initials: L
authors.lastname: Wei
authors.affiliation: Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Qinghai key laboratory Of Tibetan Medicine Pharmacology and Safety Evaluation, 810008 Xining, China; Key Laboratory of Tibetan Medicine Research, Chinese Academy of Sciences, 810008 Xining, China.
authors.forename: Yuancan
authors.initials: Y
authors.lastname: Xiao
authors.affiliation: Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Qinghai key laboratory Of Tibetan Medicine Pharmacology and Safety Evaluation, 810008 Xining, China; Key Laboratory of Tibetan Medicine Research, Chinese Academy of Sciences, 810008 Xining, China.
authors.forename: Hongtao
authors.initials: H
authors.lastname: Bi
authors.affiliation: Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Qinghai key laboratory Of Tibetan Medicine Pharmacology and Safety Evaluation, 810008 Xining, China; Key Laboratory of Tibetan Medicine Research, Chinese Academy of Sciences, 810008 Xining, China. Electronic address: hxyang@nwipb.cas.cn.
authors.forename: Hongxia
authors.initials: H
authors.lastname: Yang
authors.affiliation: Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Qinghai key laboratory Of Tibetan Medicine Pharmacology and Safety Evaluation, 810008 Xining, China; Key Laboratory of Tibetan Medicine Research, Chinese Academy of Sciences, 810008 Xining, China. Electronic address: yzdu@nwipb.cas.cn.
authors.forename: Yuzhi
authors.initials: Y
authors.lastname: Du
mesh_terms: D000596:Amino Acids / Q000032:analysis*; D000818:Animals; D002417:Cattle; D005780:Gelatin / Q000737:chemistry* / Q000145:classification / Q000302:isolation & purification; D006358:Hot Temperature; D008970:Molecular Weight; D010434:Pepsin A / Q000737:chemistry; D011489:Protein Denaturation; D055550:Protein Stability; D059748:Proteolysis; D012867:Skin / Q000737:chemistry*
chemical_list: D000596:Amino Acids; D005780:Gelatin; D010434:Pepsin A
keywords: Physicochemical and functional properties; Thermotolerance; Yak skin gelatin