title: DNA Methylation of Cellular Retinoic Acid-Binding Proteins in Cervical Cancer.
abstract: This study determined the methylation status of cellular retinoic acid-binding protein (CRABP) gene promoters and associated them with demographic characteristics, habits, and the presence of human papilloma virus (HPV) in patients with cervical cancer (CC), low and high squamous intraepithelial lesions, and no intraepithelial lesion. Women (n = 158) were selected from the Colposcopy Clinic of Sanitary Jurisdiction II in Ciudad Juarez, Chihuahua, Mexico. Demographic characteristics and habit information were collected. Cervical biopsy and endocervical scraping were used to determine methylation in promoter regions by methylation-specific polymerase chain reaction technique. We found hemi-methylation patterns in the promoter regions ofCRABP1andCRABP2; there was 28.5% hemi-methylation inCRABP1and 7.0% in that ofCRABP2. Methylation inCRABP1was associated with age (≥35 years,P= 0.002), family history of cancer (P= 0.032), the presence of HPV-16 (P= 0.013), and no alcohol intake (P= 0.035). These epigenetic changes could be involved in the CC process, andCRABP1has the potential to be a predictive molecular marker of retinoid therapy response.
authors.affiliation: Laboratorio de Biotecnología, Instituto de Ciencias Biomédicas, Universidad Autónoma de Ciudad Juárez, Ciudad Juárez, Chihuahua, México.
authors.forename: Ana L
authors.initials: AL
authors.lastname: Arellano-Ortiz
authors.affiliation: Unidad de Investigación Médica de Enfermedades Oncológicas, Hospital de Oncología, Centro Médico Nacional-Siglo XXI, IMSS, Ciudad de México, México.
authors.forename: Mauricio
authors.initials: M
authors.lastname: Salcedo-Vargas
authors.affiliation: Laboratorio de Biotecnología, Instituto de Ciencias Biomédicas, Universidad Autónoma de Ciudad Juárez, Ciudad Juárez, Chihuahua, México.
authors.forename: Claudia L
authors.initials: CL
authors.lastname: Vargas-Requena
authors.affiliation: Laboratorio de Biotecnología, Instituto de Ciencias Biomédicas, Universidad Autónoma de Ciudad Juárez, Ciudad Juárez, Chihuahua, México.
authors.forename: José A
authors.initials: JA
authors.lastname: López-Díaz
authors.affiliation: Laboratorio de Biotecnología, Instituto de Ciencias Biomédicas, Universidad Autónoma de Ciudad Juárez, Ciudad Juárez, Chihuahua, México.
authors.forename: Antonio
authors.initials: A
authors.lastname: De la Mora-Covarrubias
authors.affiliation: Laboratorio de Biotecnología, Instituto de Ciencias Biomédicas, Universidad Autónoma de Ciudad Juárez, Ciudad Juárez, Chihuahua, México.
authors.forename: Juan C
authors.initials: JC
authors.lastname: Silva-Espinoza
authors.affiliation: Laboratorio de Biotecnología, Instituto de Ciencias Biomédicas, Universidad Autónoma de Ciudad Juárez, Ciudad Juárez, Chihuahua, México.
authors.forename: Florinda
authors.initials: F
authors.lastname: Jiménez-Vega
keywords: CRABP; cervical cancer; methylation; squamous intraepithelial lesions