title: Digital PCR for Quantifying Norovirus in Oysters Implicated in Outbreaks, France.
abstract: Using samples from oysters clearly implicated in human disease, we quantified norovirus levels by using digital PCR. Concentrations varied from 43 to 1,170 RNA copies/oyster. The analysis of frozen samples from the production area showed the presence of norovirus 2 weeks before consumption.
authors.forename: David
authors.initials: D
authors.lastname: Polo
authors.forename: Julien
authors.initials: J
authors.lastname: Schaeffer
authors.forename: Nelly
authors.initials: N
authors.lastname: Fournet
authors.forename: Jean-Claude
authors.initials: JC
authors.lastname: Le Saux
authors.forename: Sylvain
authors.initials: S
authors.lastname: Parnaudeau
authors.forename: Catherine
authors.initials: C
authors.lastname: McLeod
authors.forename: Françoise S
authors.initials: FS
authors.lastname: Le Guyader
mesh_terms: D000818:Animals; D017250:Caliciviridae Infections / Q000453:epidemiology* / Q000821:virology*; D004196:Disease Outbreaks; D005506:Food Contamination; D059022:Food Safety; D005517:Foodborne Diseases / Q000453:epidemiology / Q000821:virology; D005602:France / Q000453:epidemiology; D005759:Gastroenteritis / Q000453:epidemiology* / Q000821:virology*; D005838:Genotype; D006801:Humans; D029322:Norovirus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D010125:Ostreidae / Q000821:virology*; D012367:RNA, Viral
chemical_list: D012367:RNA, Viral
keywords: France; digital PCR; enteric infections; food safety; norovirus; oysters; shellfish; viruses