title: Thin-Film Hydration Followed by Extrusion Method for Liposome Preparation.
abstract: One of the simplest ways to prepare liposomes in a research laboratory is the thin-film hydration method followed by extrusion. This method involves making a thin lipid film in a round-bottom flask by the removal of organic solvent. Upon the addition and agitation of the dispersion medium, heterogeneous liposomes are formed. Finally, after extrusion through polycarbonate membranes, homogeneous small liposomes are obtained.
authors.affiliation: School of Pharmacy - Boston, MCPHS University, 179 Longwood Ave., Boston, MA, 02115, USA. hongwei.zhang@mcphs.edu.
authors.forename: Hongwei
authors.initials: H
authors.lastname: Zhang
mesh_terms: D001671:Biochemistry / Q000379:methods*; D008081:Liposomes / Q000737:chemistry*; D010316:Particle Size; D013499:Surface Properties; D014867:Water / Q000737:chemistry*
chemical_list: D008081:Liposomes; D014867:Water
keywords: Drug delivery; Extrusion; Liposomes; Phase transition temperature; Thin-film hydration