title: Comparison between smaller ruptured intracranial aneurysm and larger un-ruptured intracranial aneurysm: gene expression profile analysis.
abstract: As it grows in size, an intracranial aneurysm (IA) is prone to rupture. In this study, we compared two extreme groups of IAs, ruptured IAs (RIAs) smaller than 10 mm and un-ruptured IAs (UIAs) larger than 10 mm, to investigate the genes involved in the facilitation and prevention of IA rupture. The aneurismal walls of 6 smaller saccular RIAs (size smaller than 10 mm), 6 larger saccular UIAs (size larger than 10 mm) and 12 paired control arteries were obtained during surgery. The transcription profiles of these samples were studied by microarray analysis. RT-qPCR was used to confirm the expression of the genes of interest. In addition, functional group analysis of the differentially expressed genes was performed. Between smaller RIAs and larger UIAs, 101 genes and 179 genes were significantly over-expressed, respectively. In addition, functional group analysis demonstrated that the up-regulated genes in smaller RIAs mainly participated in the cellular response to metal ions and inorganic substances, while most of the up-regulated genes in larger UIAs were involved in inflammation and extracellular matrix (ECM) organization. Moreover, compared with control arteries, inflammation was up-regulated and muscle-related biological processes were down-regulated in both smaller RIAs and larger UIAs. The genes involved in the cellular response to metal ions and inorganic substances may facilitate the rupture of IAs. In addition, the healing process, involving inflammation and ECM organization, may protect IAs from rupture.
authors.affiliation: Department of Neurosurgery, Beijing Tiantan Hospital, Capital Medical University, No. 6 Tiantanxili, Beijing, 100050, China.|China National Clinical Research Center for Neurological Diseases, Beijing, 100050, China.
authors.forename: Hao
authors.initials: H
authors.lastname: Li
authors.affiliation: Laborotary of Clinical Medicine Research, Beijing Tiantan Hospital, Capital Medical University, Beijing, 100050, China.
authors.forename: Haowen
authors.initials: H
authors.lastname: Li
authors.affiliation: Key Laboratory of RNA Biology, Institute of Biophysics, Chinese Academy of Sciences, Beijing, 100101, China.|Beijing Key Laboratory of Noncoding RNA, Institute of Biophysics, Chinese Academy of Sciences, Beijing, 100101, China.|University of Chinese Academy of Sciences, Beijing, 100049, China.
authors.forename: Haiyan
authors.initials: H
authors.lastname: Yue
authors.affiliation: Department of Neurosurgery, Beijing Tiantan Hospital, Capital Medical University, No. 6 Tiantanxili, Beijing, 100050, China.|China National Clinical Research Center for Neurological Diseases, Beijing, 100050, China.|Department of Neurosurgery, The Second Affiliated Hospital of Soochow University, Suzhou, 215123, China.
authors.forename: Wen
authors.initials: W
authors.lastname: Wang
authors.affiliation: Department of Neurosurgery, Beijing Tiantan Hospital, Capital Medical University, No. 6 Tiantanxili, Beijing, 100050, China.|China National Clinical Research Center for Neurological Diseases, Beijing, 100050, China.
authors.forename: Lanbing
authors.initials: L
authors.lastname: Yu
authors.affiliation: Department of Neurosurgery, Beijing Tiantan Hospital, Capital Medical University, No. 6 Tiantanxili, Beijing, 100050, China.|China National Clinical Research Center for Neurological Diseases, Beijing, 100050, China.
authors.lastname: ShuoWang
authors.affiliation: Department of Neurosurgery, Beijing Tiantan Hospital, Capital Medical University, No. 6 Tiantanxili, Beijing, 100050, China. caoyong6@hotmail.com.|China National Clinical Research Center for Neurological Diseases, Beijing, 100050, China. caoyong6@hotmail.com.
authors.forename: Yong
authors.initials: Y
authors.lastname: Cao
authors.affiliation: Department of Neurosurgery, Beijing Tiantan Hospital, Capital Medical University, No. 6 Tiantanxili, Beijing, 100050, China. zhaojz205@163.com.|China National Clinical Research Center for Neurological Diseases, Beijing, 100050, China. zhaojz205@163.com.
authors.forename: Jizong
authors.initials: J
authors.lastname: Zhao
mesh_terms: D000368:Aged; D017542:Aneurysm, Ruptured / Q000000981:diagnostic imaging / Q000235:genetics* / Q000601:surgery; D002536:Cerebral Arteries / Q000000981:diagnostic imaging; D005109:Extracellular Matrix / Q000473:pathology; D005260:Female; D015870:Gene Expression / Q000187:drug effects; D020869:Gene Expression Profiling; D006801:Humans; D007249:Inflammation / Q000473:pathology; D002532:Intracranial Aneurysm / Q000000981:diagnostic imaging / Q000235:genetics* / Q000601:surgery; D008297:Male; D008670:Metals / Q000494:pharmacology; D008875:Middle Aged; D060888:Real-Time Polymerase Chain Reaction; D015854:Up-Regulation / Q000235:genetics
chemical_list: D008670:Metals
keywords: Healing process; Inflammation; Intracranial aneurysms; Pathogenesis; Rupture