title: Oxidative stress induced autophagy in cancer associated fibroblast enhances proliferation and metabolism of colorectal cancer cells.
abstract: Tumors are comprised of malignant cancer cells and stromal cells which constitute the tumor microenvironment (TME). Previous studies have shown that cancer associated fibroblast (CAF) in TME is an important promoter of tumor initiation and progression. However, the underlying molecular mechanisms by which CAFs influence the growth of colorectal cancer cells (CRCs) have not been clearly elucidated. In this study, by using a non-contact co-culture system between human colorectal fibroblasts (CCD-18-co) and CRCs (LoVo, SW480, and SW620), we found that fibroblasts existing in tumor microenvironment positively influenced the metabolism of colorectal cancer cells, through its autophagy and oxidative stress pathway which were initially induced by neighboring tumor cells. Therefore, our data provided a novel possibility to develop fibroblasts as a potential target to treat CRC.
authors.affiliation: a Department of Biochemistry and Molecular Biology , Shandong University School of Medicine , Jinan , Shandong , China.|b Department of Neurosurgery , Qilu Hospital of Shandong University and Brain Science Research Institute, Shandong University , Jinan , China.
authors.forename: Wenjing
authors.initials: W
authors.lastname: Zhou
authors.affiliation: c Department of Gastroenterology , 456 Hospital of PLA , Jinan , Shandong , China.
authors.forename: Gang
authors.initials: G
authors.lastname: Xu
authors.affiliation: a Department of Biochemistry and Molecular Biology , Shandong University School of Medicine , Jinan , Shandong , China.
authors.forename: Yunqiu
authors.initials: Y
authors.lastname: Wang
authors.affiliation: d The First Affiliated Hospital of Anhui Medical University , Hefei , Anhui , China.
authors.forename: Ziao
authors.initials: Z
authors.lastname: Xu
authors.affiliation: a Department of Biochemistry and Molecular Biology , Shandong University School of Medicine , Jinan , Shandong , China.
authors.forename: Xiaofei
authors.initials: X
authors.lastname: Liu
authors.affiliation: a Department of Biochemistry and Molecular Biology , Shandong University School of Medicine , Jinan , Shandong , China.
authors.forename: Xia
authors.initials: X
authors.lastname: Xu
authors.affiliation: a Department of Biochemistry and Molecular Biology , Shandong University School of Medicine , Jinan , Shandong , China.
authors.forename: Guijie
authors.initials: G
authors.lastname: Ren
authors.affiliation: a Department of Biochemistry and Molecular Biology , Shandong University School of Medicine , Jinan , Shandong , China.
authors.forename: Keli
authors.initials: K
authors.lastname: Tian
mesh_terms: D000111:Acetylcysteine / Q000494:pharmacology; D000225:Adenine / Q000031:analogs & derivatives / Q000494:pharmacology; D001343:Autophagy / Q000187:drug effects; D015153:Blotting, Western; D000072645:Cancer-Associated Fibroblasts / Q000378:metabolism* / Q000473:pathology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D018920:Coculture Techniques; D015179:Colorectal Neoplasms / Q000378:metabolism* / Q000473:pathology*; D006801:Humans; D018384:Oxidative Stress / Q000187:drug effects
chemical_list: C025946:3-methyladenine; D000225:Adenine; D000111:Acetylcysteine
keywords: 3-MA; Colorectal cancer; NAC; autophagy; co-culture; metabolism; oxidative stress