title: Rhamnolipids Produced by IndigenousAcinetobacter juniifrom Petroleum Reservoir and its Potential in Enhanced Oil Recovery.
abstract: Biosurfactant producers are crucial for incremental oil production in microbial enhanced oil recovery (MEOR) processes. The isolation of biosurfactant-producing bacteria from oil reservoirs is important because they are considered suitable for the extreme conditions of the reservoir. In this work, a novel biosurfactant-producing strainAcinetobacter juniiBD was isolated from a reservoir to reduce surface tension and emulsify crude oil. The biosurfactants produced by the strain were purified and then identified via electrospray ionization-Fourier transform ion cyclotron resonance mass spectrometry (ESI FT-ICR-MS). The biosurfactants generated by the strain were concluded to be rhamnolipids, the dominant rhamnolipids were C26H48O9, C28H52O9, and C32H58O13. The optimal carbon source and nitrogen source for biomass and biosurfactant production were NaNO3and soybean oil. The results showed that the content of acid components increased with the progress of crude oil biodegradation. A glass micromodel test demonstrated that the strain significantly increased oil recovery through interfacial tension reduction, wettability alteration and the mobility of microorganisms. In summary, the findings of this study indicate that the newly developed BD strain and its metabolites have great potential in MEOR.
authors.affiliation: State Key Laboratory of Heavy Oil Processing, College of Chemical Engineering, China University of Petroleum Beijing, China.
authors.forename: Hao
authors.initials: H
authors.lastname: Dong
authors.affiliation: Power Environmental Energy Research Institute, Covina CA, USA.
authors.forename: Wenjie
authors.initials: W
authors.lastname: Xia
authors.affiliation: State Key Laboratory of Heavy Oil Processing, College of Chemical Engineering, China University of Petroleum Beijing, China.
authors.forename: Honghong
authors.initials: H
authors.lastname: Dong
authors.affiliation: College of Chemistry and Environmental Engineering, Yangtze University Jingzhou, China.
authors.forename: Yuehui
authors.initials: Y
authors.lastname: She
authors.affiliation: State Key Laboratory of Heavy Oil Processing, College of Chemical Engineering, China University of Petroleum Beijing, China.
authors.forename: Panfeng
authors.initials: P
authors.lastname: Zhu
authors.affiliation: State Key Laboratory of Heavy Oil Processing, College of Chemical Engineering, China University of Petroleum Beijing, China.
authors.forename: Kang
authors.initials: K
authors.lastname: Liang
authors.affiliation: State Key Laboratory of Heavy Oil Processing, College of Chemical Engineering, China University of Petroleum Beijing, China.
authors.forename: Zhongzhi
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: Dalian Design Branch, China Petroleum Engineering and Construction Corporation Dalian, China.
authors.forename: Chuanfu
authors.initials: C
authors.lastname: Liang
authors.affiliation: State Key Laboratory of Heavy Oil Processing, Faculty of Sciences, China University of Petroleum Beijing, China.
authors.forename: Zhaozheng
authors.initials: Z
authors.lastname: Song
authors.affiliation: State Key Laboratory of Heavy Oil Processing, College of Chemical Engineering, China University of Petroleum Beijing, China.
authors.forename: Shanshan
authors.initials: S
authors.lastname: Sun
authors.affiliation: School of Mechanical, Materials and Mechatronic Engineering, University of Wollongong, Wollongong NSW, Australia.
authors.forename: Guangqing
authors.initials: G
authors.lastname: Zhang
keywords: Acinetobacter junii; ESI FT-ICR-MS; biodegradation; biosurfactant; microbial enhanced oil recovery; polar components; visualization micromodel