title: Development of bone-like zirconium oxide nanoceramic modified chitosan based porous nanocomposites for biomedical application.
abstract: Here, zirconium oxide nanoparticles (ZrO2NPs) were incorporated for the first time in organic-inorganic hybrid composites containing chitosan, poly(ethylene glycol) and nano-hydroxypatite (CS-PEG-HA) to develop bone-like nanocomposites for bone tissue engineering application. These nanocomposites were characterized by FT-IR, XRD, TEM combined with SAED. SEM images and porosity measurements revealed highly porous structure having pore size of less than 1μm to 10μm. Enhanced water absorption capacity and mechanical strengths were obtained compared to previously reported CS-PEG-HA composite after addition of 0.1-0.3wt% of ZrO2NPs into these nanocomposites. The mechanical strengths and porosities were similar to that of human spongy bone. Strong antimicrobial effects against gram-negative and gram-positive bacterial strains were also observed. Along with getting low alkalinity pH (7.4) values, similar to the pH of human plasma, hemocompatibility and cytocompatibility with osteoblastic MG-63 cells were also established for these nanocomposites. Addition of 15wt% HA-ZrO2(having 0.3wt% ZrO2NPs) into CS-PEG (55:30wt%) composite resulted in greatest mechanical strength, porosity, antimicrobial property and cytocompatibility along with suitable water absorption capacity and compatibility with human pH and blood. Thus, this nanocomposite could serve as a potential candidate to be used for bone tissue engineering.
authors.affiliation: Department of Polymer Science and Technology, University of Calcutta, 92 A.P.C. Road, Kolkata, 700009, India.
authors.forename: Arundhati
authors.initials: A
authors.lastname: Bhowmick
authors.affiliation: Department of Polymer Science and Technology, University of Calcutta, 92 A.P.C. Road, Kolkata, 700009, India.
authors.forename: Nilkamal
authors.initials: N
authors.lastname: Pramanik
authors.affiliation: Department of Polymer Science and Technology, University of Calcutta, 92 A.P.C. Road, Kolkata, 700009, India.
authors.forename: Piyali
authors.initials: P
authors.lastname: Jana
authors.affiliation: Department of Polymer Science and Technology, University of Calcutta, 92 A.P.C. Road, Kolkata, 700009, India.
authors.forename: Tapas
authors.initials: T
authors.lastname: Mitra
authors.affiliation: Microbiology Division, CSIR-Central Leather Research Institute, Adyar, Chennai, 600020, Tamil Nadu, India.
authors.forename: Arumugam
authors.initials: A
authors.lastname: Gnanamani
authors.affiliation: Department of Chemical Engineering, University of Calcutta, 92 A.P.C. Road, Kolkata, 700009, India.
authors.forename: Manas
authors.initials: M
authors.lastname: Das
authors.affiliation: Department of Polymer Science and Technology, University of Calcutta, 92 A.P.C. Road, Kolkata, 700009, India; Department of Chemical Engineering, Indian Institute of Technology, Roorkee, 247667, India. Electronic address: ppk923@yahoo.com.
authors.forename: Patit Paban
authors.initials: PP
authors.lastname: Kundu
mesh_terms: D065966:Absorption, Physicochemical; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology / Q000633:toxicity; D001672:Biocompatible Materials / Q000737:chemistry* / Q000494:pharmacology* / Q000633:toxicity; D001842:Bone and Bones / Q000166:cytology / Q000187:drug effects; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D002516:Ceramics / Q000737:chemistry*; D048271:Chitosan / Q000737:chemistry*; D006461:Hemolysis / Q000187:drug effects; D006801:Humans; D008422:Materials Testing; D055595:Mechanical Phenomena; D053761:Nanocomposites / Q000737:chemistry*; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects; D016062:Porosity; D023822:Tissue Engineering; D054457:Tissue Scaffolds / Q000737:chemistry; D014867:Water / Q000737:chemistry; D015040:Zirconium / Q000737:chemistry*
chemical_list: D000900:Anti-Bacterial Agents; D001672:Biocompatible Materials; D014867:Water; D048271:Chitosan; D015040:Zirconium; C028541:zirconium oxide
keywords: Chitosan; Hydroxyapatite; Nanocomposites; Poly(ethylene glycol); Zirconium oxide