title: Vaccination againstCorynebacterium pseudotuberculosisinfections controlling caseous lymphadenitis (CLA) and oedematousskin disease.
abstract: Corynebacterium pseudotuberculosis(C. pseudotuberculosis) is a causative organism of caseous lymphadenitis (CLA) in sheep and acute disease in buffaloes known as oedematous skin disease (OSD). Human affected with the disease show liver abscess and abscess in the internal lymph nodes. The vaccination against CLA up till now occurs by using formalin inactivated whole cells of biovar 1 (sheep strain). Combined vaccine composed of formalin inactivated whole cells of sheep strain and recombinant phospholipase D (rPLD) and another vaccine composed of formalin inactivated whole cells (buffalo origin) and rPLD were prepared in Biotechnology center for services and Researches laboratory at Cairo university and applied for protection against CLA. Both vaccines induced complete protection (100%) against challenge with virulent biovar 1 or biovar 2. Also vaccination against OSD was performed by two types of vaccines. Vaccine-1 was composed of formalin inactivated whole cell biovar 1 combined with rPLD and the second vaccine was composed of formalin inactivated whole cells of biovar 2 combined with rPLD. No lesions developed in vaccinated and non vaccinated buffaloes challenged withC. pseudotuberculosisbiovar revealing that biovar 1C. pseudotuberculosisis not infective for buffaloes. Buffaloes vaccinated with the second vaccine and control non vaccinated animals challenged with biovar 2 (buffalo origin) resulted in development of OSD in all animals. This indicates that OSD results due to production of toxin (s) other than PLD. Discovering this toxin (s) is of value in formulation of a future vaccine against OSD.
authors.affiliation: Department of Botany and Microbiology, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi Arabia; Department of Microbiology, Faculty of Veterinary Medicine, Cairo University, Egypt.
authors.forename: Ihab M
authors.initials: IM
authors.lastname: Moussa
authors.affiliation: Department of Microbiology and Immunology, College of Pharmacy, AL Azhar University, Cairo, Egypt.
authors.forename: Mohamed S
authors.initials: MS
authors.lastname: Ali
authors.affiliation: Department of Microbiology, Faculty of Veterinary Medicine, Cairo University, Egypt; Department of Health Science, College of Applied Studies and Community Service, King Saud University, P.O. Box 22459, Riyadh 11495, Saudi Arabia.
authors.forename: Ashgan M
authors.initials: AM
authors.lastname: Hessain
authors.affiliation: Department of Biology, Faculty of Science, King Abdulaziz University, P.O. Box 80203, Jeddah, Saudi Arabia.
authors.forename: Saleh A
authors.initials: SA
authors.lastname: Kabli
authors.affiliation: Department of Medical Technology/Microbiology, College of Applied Medical Science, Taibah University, Madinah, Saudi Arabia.
authors.forename: Hassan A
authors.initials: HA
authors.lastname: Hemeg
authors.affiliation: Department of Microbiology, Faculty of Veterinary Medicine, Cairo University, Egypt.
authors.forename: Salha Abdelkareem
authors.initials: SA
authors.lastname: Selim
keywords: Caseous lymphadenitis; Corynebacterium pseudotuberculosis; Oedematous skin disease; Recombinant phospholipase D; Vaccination