title: Proteomic Profiling for Identification of Novel Biomarkers Differentially Expressed in Human Ovaries from Polycystic Ovary Syndrome Patients.
abstract: OBJECTIVE
  To identify differential protein expression pattern associated with polycystic ovary syndrome (PCOS).
  
  
  METHODS
  Twenty women were recruited for the study, ten with PCOS as a test group and ten without PCOS as a control group. Differential in-gel electrophoresis (DIGE) analysis and mass spectroscopy were employed to identify proteins that were differentially expressed between the PCOS and normal ovaries. The differentially expressed proteins were further validated by western blot (WB) and immunohistochemistry (IHC).
  
  
  RESULTS
  DIGE analysis revealed eighteen differentially expressed proteins in the PCOS ovaries of which thirteen were upregulated, and five downregulated. WB and IHC confirmed the differential expression of membrane-associated progesterone receptor component 1 (PGRMC1), retinol-binding protein 1 (RBP1), heat shock protein 90B1, calmodulin 1, annexin A6, and tropomyosin 2. Also, WB analysis revealed significantly (P<0.05) higher expression of PGRMC1 and RBP1 in PCOS ovaries as compared to the normal ovaries. The differential expression of the proteins was also validated by IHC.
  
  
  CONCLUSIONS
  The present study identified novel differentially expressed proteins in the ovarian tissues of women with PCOS that can serve as potential biomarkers for the diagnosis and development of novel therapeutics for the treatment of PCOS using molecular interventions.
authors.affiliation: Department of Obstetrics and Gynecology, Guangdong Women and Children Hospital, Guangzhou, 510010, China.
authors.forename: Li
authors.initials: L
authors.lastname: Li
authors.affiliation: Department of Pathology, Guangdong Women and Children Hospital, Guangzhou, 510010, China.
authors.forename: Jiangyu
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Department of Obstetrics and Gynecology, Guangdong Women and Children Hospital, Guangzhou, 510010, China.
authors.forename: Qingshan
authors.initials: Q
authors.lastname: Deng
authors.affiliation: Department of Obstetrics and Gynecology, Guangdong Women and Children Hospital, Guangzhou, 510010, China.
authors.forename: Jieming
authors.initials: J
authors.lastname: Li
authors.affiliation: The Third Affiliated Hospital of Southern Medical University, Guangzhou, 510515, China.
authors.forename: Zhengfen
authors.initials: Z
authors.lastname: Li
authors.affiliation: Laboratory of Chinese Medicine Quality Research, Macau University of Science and Technology, Macau, 00853, China.
authors.forename: Yao
authors.initials: Y
authors.lastname: Xiao
authors.affiliation: Department of Pathophysiology, Southern Medical University, Guangzhou, 510515, China.
authors.forename: Shuiwang
authors.initials: S
authors.lastname: Hu
authors.affiliation: Department of Obstetrics and Gynecology, Guangdong Women and Children Hospital, Guangzhou, 510010, China.
authors.forename: Tiantian
authors.initials: T
authors.lastname: Li
authors.affiliation: Department of Obstetrics and Gynecology, Guangdong Women and Children Hospital, Guangzhou, 510010, China.
authors.forename: Qiuxiao
authors.initials: Q
authors.lastname: Tan
authors.affiliation: Department of Obstetrics and Gynecology, Guangdong Women and Children Hospital, Guangzhou, 510010, China.
authors.forename: Xiaofang
authors.initials: X
authors.lastname: Li
authors.affiliation: Department of Obstetrics and Gynecology, Guangdong Women and Children Hospital, Guangzhou, 510010, China.
authors.forename: Bingshu
authors.initials: B
authors.lastname: Luo
authors.affiliation: Laboratory of Chinese Medicine Quality Research, Macau University of Science and Technology, Macau, 00853, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Mo
mesh_terms: D000328:Adult; D015415:Biomarkers / Q000378:metabolism*; D015153:Blotting, Western; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D010053:Ovary / Q000378:metabolism*; D011085:Polycystic Ovary Syndrome / Q000378:metabolism*; D040901:Proteomics / Q000379:methods*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D055815:Young Adult
chemical_list: D015415:Biomarkers