title: Prevalence of Colistin Resistance Gene mcr-1 and Absence of mcr-2 in Escherichia coli Isolated from Healthy Food-Producing Animals in Japan.
abstract: We screened mcr-1 and mcr-2 genes in 9,306 Escherichia coli strains isolated from healthy animals in the Japanese Veterinary Antimicrobial Resistance Monitoring (JVARM) system. mcr-1 was detected in 39 strains (5, 20, and 14 strains isolated from cattle, swine, and broilers, respectively), whereas mcr-2 was not detected. mcr-2 was also not detected with the investigation sequence homology search against our curated GenEpid-J database.
authors.affiliation: National Veterinary Assay Laboratory, Ministry of Agriculture, Forestry and Fisheries, Kokubunji, Japan michiko_kawanishi700@maff.go.jp.
authors.forename: Michiko
authors.initials: M
authors.lastname: Kawanishi
authors.affiliation: National Veterinary Assay Laboratory, Ministry of Agriculture, Forestry and Fisheries, Kokubunji, Japan.
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Abo
authors.affiliation: National Veterinary Assay Laboratory, Ministry of Agriculture, Forestry and Fisheries, Kokubunji, Japan.
authors.forename: Manao
authors.initials: M
authors.lastname: Ozawa
authors.affiliation: National Veterinary Assay Laboratory, Ministry of Agriculture, Forestry and Fisheries, Kokubunji, Japan.
authors.forename: Mariko
authors.initials: M
authors.lastname: Uchiyama
authors.affiliation: National Veterinary Assay Laboratory, Ministry of Agriculture, Forestry and Fisheries, Kokubunji, Japan.
authors.forename: Takahiro
authors.initials: T
authors.lastname: Shirakawa
authors.affiliation: Department of Bacteriology II, National Institute of Infectious Diseases, Musashimurayama, Japan.
authors.forename: Satowa
authors.initials: S
authors.lastname: Suzuki
authors.affiliation: Department of Bacteriology II, National Institute of Infectious Diseases, Musashimurayama, Japan.
authors.forename: Ayaka
authors.initials: A
authors.lastname: Shima
authors.affiliation: Pathogen Genomics Center, National Institute of Infectious Diseases, Shinjyuku-ku, Japan.
authors.forename: Akifumi
authors.initials: A
authors.lastname: Yamashita
authors.affiliation: Pathogen Genomics Center, National Institute of Infectious Diseases, Shinjyuku-ku, Japan.
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Sekizuka
authors.affiliation: Pathogen Genomics Center, National Institute of Infectious Diseases, Shinjyuku-ku, Japan.
authors.forename: Kengo
authors.initials: K
authors.lastname: Kato
authors.affiliation: Pathogen Genomics Center, National Institute of Infectious Diseases, Shinjyuku-ku, Japan.
authors.forename: Makoto
authors.initials: M
authors.lastname: Kuroda
authors.affiliation: National Veterinary Assay Laboratory, Ministry of Agriculture, Forestry and Fisheries, Kokubunji, Japan.
authors.forename: Ryoji
authors.initials: R
authors.lastname: Koike
authors.affiliation: National Veterinary Assay Laboratory, Ministry of Agriculture, Forestry and Fisheries, Kokubunji, Japan.
authors.forename: Mayumi
authors.initials: M
authors.lastname: Kijima
mesh_terms: D000822:Animal Husbandry; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D002417:Cattle; D002645:Chickens; D003091:Colistin / Q000494:pharmacology; D024881:Drug Resistance, Bacterial / Q000235:genetics*; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification; D004927:Escherichia coli Infections / Q000453:epidemiology / Q000382:microbiology / Q000662:veterinary*; D029968:Escherichia coli Proteins / Q000235:genetics* / Q000378:metabolism; D015870:Gene Expression; D007564:Japan / Q000453:epidemiology; D008460:Meat / Q000382:microbiology*; D011201:Poultry Diseases / Q000453:epidemiology* / Q000382:microbiology; D015995:Prevalence; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism; D013552:Swine; D013553:Swine Diseases / Q000453:epidemiology* / Q000382:microbiology
chemical_list: D000900:Anti-Bacterial Agents; D029968:Escherichia coli Proteins; C000614037:MCR-1 protein, E coli; D020033:Protein Isoforms; D003091:Colistin
keywords: GenEpid-J; Japanese Veterinary Antimicrobial Resistance Monitoring system; colistin resistance; food-producing animals; mcr-1; mcr-2