title: Fluorometric Analysis of Individual Cationic Lipid-DNA Complexes.
abstract: Lipoplex preparations are heterogeneous mixtures of lipoplex particles of different structure. Because these structures determine the efficiency of genetic material delivery, it is important to characterize the distribution of particles of different types in lipoplex preparations with good statistics. We describe the application of flow fluorometry which allow producing such distributions (in terms of lipoplex particle size and composition) within minutes using basic flow cytometer (Pozharski and MacDonald, Anal Biochem 341:230-240, 2005).
authors.affiliation: Department of Biochemistry and Molecular Biology, Center for Biomolecular Therapeutics, Institute for Bioscience and Biotechnology Research, University of Maryland School of Medicine, 9600 Gudelsky Dr., 4127D Bldg 2, Rockville, MD, 20850, USA. EPozharskiy@som.umaryland.edu.
authors.forename: Edwin
authors.initials: E
authors.lastname: Pozharski
mesh_terms: D002412:Cations; D004247:DNA / Q000737:chemistry*; D005470:Fluorometry / Q000379:methods*; D008055:Lipids / Q000737:chemistry*; D008081:Liposomes / Q000737:chemistry; D013194:Staining and Labeling; D013223:Statistics as Topic
chemical_list: D002412:Cations; D008055:Lipids; D008081:Liposomes; D004247:DNA
keywords: Cationic lipid; Flow cytometry; Heterogeneity; Lipoplex; Single particle detection; Size-stoichiometry distribution