title: Capsular stroke modeling based on somatotopic mapping of motor fibers.
abstract: Recently, several capsular stroke models have been reported with different targets of destruction. This study was performed to establish an accurate internal capsule (IC) target for capsular stroke modeling in rats. We injected adeno-associated virus serotype 5 (AAV)-CaMKII-EYFP into forelimb motor cortex and AAV-CaMKII-mCherry into hindlimb motor cortex (n = 9) to anterogradely trace the pyramidal fibers and map their somatotopic distribution in the IC. On the basis of the neural tracing results, we created photothrombotic infarct lesions in rat forelimb and hindlimb motor fiber (FMF and HMF) areas of the IC (n = 29) and assessed motor behavior using a forelimb-use asymmetry test, a foot-fault test, and a single-pellet reaching test. We found that the FMFs and HMFs were primarily distributed in the inferior portion of the posterior limb of the IC, with the FMFs located largely ventral to the HMFs but with an area of partial overlap. Photothrombotic lesions in the FMF area resulted in persistent motor deficits. In contrast, lesions in the HMF area did not result in persistent motor deficits. These results indicate that identification of the somatotopic distribution of pyramidal fibers is critical for accurate targeting in animal capsular stroke models: only infarcts in the FMF area resulted in long-lasting motor deficits.
authors.affiliation: 1 Department of Biomedical Science and Engineering, Gwangju Institute of Science and Technology, Gwangju, Republic of Korea.
authors.forename: Hanlim
authors.initials: H
authors.lastname: Song
authors.affiliation: 1 Department of Biomedical Science and Engineering, Gwangju Institute of Science and Technology, Gwangju, Republic of Korea.
authors.forename: Wonbin
authors.initials: W
authors.lastname: Jung
authors.affiliation: 1 Department of Biomedical Science and Engineering, Gwangju Institute of Science and Technology, Gwangju, Republic of Korea.
authors.forename: Eulgi
authors.initials: E
authors.lastname: Lee
authors.affiliation: 1 Department of Biomedical Science and Engineering, Gwangju Institute of Science and Technology, Gwangju, Republic of Korea.
authors.forename: Ji-Young
authors.initials: JY
authors.lastname: Park
authors.affiliation: 2 Department of Physiology, Wonkwang University School of Medicine, Iksan, Republic of Korea.
authors.forename: Min Sun
authors.initials: MS
authors.lastname: Kim
authors.affiliation: 3 Department of Pathology, Chonnam National University Medical School, Gwangju, Republic of Korea.
authors.forename: Min-Cheol
authors.initials: MC
authors.lastname: Lee
authors.affiliation: 1 Department of Biomedical Science and Engineering, Gwangju Institute of Science and Technology, Gwangju, Republic of Korea.|4 Department of Neurosurgery, Presbyterian Medical Center, Jeonju, Republic of Korea.
authors.forename: Hyoung-Ihl
authors.initials: HI
authors.lastname: Kim
mesh_terms: D000818:Animals; D001522:Behavior, Animal / Q000502:physiology; D000229:Dependovirus; D004195:Disease Models, Animal; D005552:Forelimb / Q000294:innervation; D006614:Hindlimb / Q000294:innervation; D020772:Internal Capsule / Q000473:pathology / Q000503:physiopathology; D008297:Male; D009043:Motor Activity / Q000502:physiology; D009044:Motor Cortex / Q000473:pathology* / Q000503:physiopathology; D009412:Nerve Fibers / Q000502:physiology*; D009434:Neural Pathways / Q000502:physiology*; D017207:Rats, Sprague-Dawley; D020521:Stroke / Q000473:pathology* / Q000503:physiopathology; D000071939:Stroke Rehabilitation
keywords: Stroke; internal capsule; neural tracing; photothrombosis; post-stroke recovery