title: Phospholipase D activity couples plasma membrane endocytosis with retromer dependent recycling.
abstract: During illumination, the light-sensitive plasma membrane (rhabdomere) ofDrosophilaphotoreceptors undergoes turnover with consequent changes in size and composition. However, the mechanism by which illumination is coupled to rhabdomere turnover remains unclear. We find that photoreceptors contain a light-dependent phospholipase D (PLD) activity. During illumination, loss of PLD resulted in an enhanced reduction in rhabdomere size, accumulation of Rab7 positive, rhodopsin1-containing vesicles (RLVs) in the cell body and reduced rhodopsin protein. These phenotypes were associated with reduced levels of phosphatidic acid, the product of PLD activity and were rescued by reconstitution with catalytically active PLD. In wild-type photoreceptors, during illumination, enhanced PLD activity was sufficient to clear RLVs from the cell body by a process dependent on Arf1-GTP levels and retromer complex function. Thus, during illumination, PLD activity couples endocytosis of RLVs with their recycling to the plasma membrane thus maintaining plasma membrane size and composition.
authors.affiliation: National Centre for Biological Sciences, Bangalore, India.|Shanmugha Arts, Science, Technology & Research Academy, Thanjavur, India.
authors.forename: Rajan
authors.identifier: 0000-0002-9511-5729
authors.initials: R
authors.lastname: Thakur
authors.affiliation: National Centre for Biological Sciences, Bangalore, India.|Manipal University, Karnataka, India.
authors.forename: Aniruddha
authors.initials: A
authors.lastname: Panda
authors.affiliation: Inositide Laboratory, Babraham Institute, Cambridge, United Kingdom.
authors.forename: Elise
authors.initials: E
authors.lastname: Coessens
authors.affiliation: National Centre for Biological Sciences, Bangalore, India.
authors.forename: Nikita
authors.initials: N
authors.lastname: Raj
authors.affiliation: National Centre for Biological Sciences, Bangalore, India.
authors.forename: Shweta
authors.initials: S
authors.lastname: Yadav
authors.affiliation: National Centre for Biological Sciences, Bangalore, India.
authors.forename: Sruthi
authors.initials: S
authors.lastname: Balakrishnan
authors.affiliation: Inositide Laboratory, Babraham Institute, Cambridge, United Kingdom.
authors.forename: Qifeng
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: Inositide Laboratory, Babraham Institute, Cambridge, United Kingdom.
authors.forename: Plamen
authors.initials: P
authors.lastname: Georgiev
authors.affiliation: National Centre for Biological Sciences, Bangalore, India.
authors.forename: Bishal
authors.initials: B
authors.lastname: Basak
authors.affiliation: National Centre for Biological Sciences, Bangalore, India.
authors.forename: Renu
authors.initials: R
authors.lastname: Pasricha
authors.affiliation: Inositide Laboratory, Babraham Institute, Cambridge, United Kingdom.
authors.forename: Michael Jo
authors.initials: MJ
authors.lastname: Wakelam
authors.affiliation: Inositide Laboratory, Babraham Institute, Cambridge, United Kingdom.
authors.forename: Nicholas T
authors.identifier: 0000-0001-9397-2914
authors.initials: NT
authors.lastname: Ktistakis
authors.affiliation: National Centre for Biological Sciences, Bangalore, India.
authors.forename: Padinjat
authors.identifier: 0000-0003-3578-6413
authors.initials: P
authors.lastname: Raghu
mesh_terms: D020823:ADP-Ribosylation Factor 1 / Q000235:genetics / Q000378:metabolism; D000818:Animals; D002462:Cell Membrane / Q000378:metabolism* / Q000528:radiation effects / Q000648:ultrastructure; D022162:Cytoplasmic Vesicles / Q000378:metabolism; D029721:Drosophila Proteins / Q000235:genetics / Q000378:metabolism*; D004331:Drosophila melanogaster / Q000166:cytology / Q000201:enzymology* / Q000235:genetics / Q000528:radiation effects; D004705:Endocytosis / Q000528:radiation effects*; D015870:Gene Expression; D005816:Genetic Complementation Test; D006160:Guanosine Triphosphate / Q000378:metabolism; D008027:Light; D010712:Phosphatidic Acids / Q000378:metabolism; D010739:Phospholipase D / Q000235:genetics / Q000378:metabolism*; D010775:Photic Stimulation; D017956:Photoreceptor Cells, Invertebrate / Q000378:metabolism* / Q000528:radiation effects / Q000648:ultrastructure; D012243:Rhodopsin / Q000235:genetics / Q000378:metabolism; D014785:Vision, Ocular / Q000502:physiology; D020691:rab GTP-Binding Proteins / Q000235:genetics / Q000378:metabolism; D000090802:rab7 GTP-Binding Proteins
chemical_list: D029721:Drosophila Proteins; D010712:Phosphatidic Acids; D000090802:rab7 GTP-Binding Proteins; D006160:Guanosine Triphosphate; D012243:Rhodopsin; D010739:Phospholipase D; D020823:ADP-Ribosylation Factor 1; D020691:rab GTP-Binding Proteins
keywords: Arf1; D. melanogaster; biochemistry; cell biology; lipid signalling; phosphatidic acid; phospholipase D; retromer; rhodopsin