title: Dietary sodium propionate affects mucosal immune parameters, growth and appetite related genes expression: Insights from zebrafish model.
abstract: Propionate is a short-chain fatty acid (SCFA) that improves physiological and pathophysiological properties. However, there is limited information available about the effects of SCFAs on mucosal immune parameters as well as growth and appetite related genes expression. The aim of the present study was to evaluate the effect of sodium propionate (SP) intake on the mucosal immune parameters, growth and appetite related genes expression using zebrafish (Danio rerio) as model organism. Zebrafish fed control or diet supplemented with different levels (0.5, 1 and 2%) of SP for 8weeks. At the end of feeding trial, the expression of the key genes related to growth and appetite (GH, IGF1, MYSTN and Ghrl) was evaluated. Also, mucosal immune parameters (Total Ig, lysozyme and protease activity) were studied in skin mucus of zebrafish. The results showed that dietary administration of SP significantly (P<0.05) up-regulated the expression of GH, IGF1 and down-regulated MYSTN gene. Also, feeding zebrafish with SP supplemented diet significantly increased appetite related gene expression (P<0.05) with a more pronounced effect in higher inclusion levels. Compared with control group, the expression of appetite related gene (Ghrl) was remarkably (P<0.05) higher in SP fed zebrafish. Also, elevated mucosal immune parameters was observed in zebrafish fed SP supplemented diet. The present results revealed beneficial effects of dietary SP on mucosal immune response and growth and appetite related genes expression. These results also highlighted the potential use of SP as additive in human diets.
authors.affiliation: Department of Fisheries, Faculty of Fisheries and Environmental Sciences, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran. Electronic address: Hoseinifar@gau.ac.ir.
authors.forename: Seyed Hossein
authors.initials: SH
authors.lastname: Hoseinifar
authors.affiliation: Department of Fisheries, Faculty of Fisheries and Environmental Sciences, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran.
authors.forename: Roghieh
authors.initials: R
authors.lastname: Safari
authors.affiliation: Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran.
authors.forename: Maryam
authors.initials: M
authors.lastname: Dadar
mesh_terms: D000821:Animal Feed; D000818:Animals; D001066:Appetite / Q000235:genetics*; D005786:Gene Expression Regulation / Q000187:drug effects*; D018928:Immunity, Mucosal / Q000235:genetics*; D011422:Propionates / Q000494:pharmacology*; D012982:Sodium, Dietary / Q000494:pharmacology*; D015027:Zebrafish / Q000235:genetics* / Q000254:growth & development / Q000276:immunology; D029961:Zebrafish Proteins / Q000235:genetics*
chemical_list: D011422:Propionates; D012982:Sodium, Dietary; D029961:Zebrafish Proteins; C514135:sodium propionate
keywords: Appetite; Growth hormone; Mucosal immunity; Myostatin; Physiological response; Short-chain fatty acids