title: Quantitative proteomic analysis of age-related subventricular zone proteins associated with neurodegenerative disease.
abstract: Aging is characterized by a progressive decline in the function of adult tissues which can lead to neurodegenerative disorders. However, little is known about the correlation between protein changes in the subventricular zone (SVZ) and neurodegenerative diseases with age. In the present study, neural stem cells (NSCs) were derived from the SVZ on postnatal 7 d, 1 m, and 12 m-old mice. With age, NSCs exhibited increased SA-β-gal activity and decreased proliferation and pool size in the SVZ zone, and were associated with elevated inflammatory chemokines and cytokines. Furthermore, quantitative proteomics and ingenuity pathway analysis were used to evaluate the significant age-related alterations in proteins and their functions. Some downregulated proteins such as DPYSL2, TPI1, ALDH, and UCHL1 were found to play critical roles in the neurological disease and PSMA1, PSMA3, PSMC2, PSMD11, and UCHL1 in protein homeostasis. Taken together, we have provided valuable insight into the cellular and molecular processes that underlie aging-associated declines in SVZ neurogenesis for the early detection of differences in gene expression and the potential risk of neurological disease, which is beneficial in the prevention of the diseases.
authors.affiliation: East Hospital, Tongji University School of Medicine, Shanghai, China.
authors.forename: Xianli
authors.initials: X
authors.lastname: Wang
authors.affiliation: East Hospital, Tongji University School of Medicine, Shanghai, China.|Department of Anatomy, Nantong University, Nantong, Jiangsu, China.
authors.forename: Chuanming
authors.initials: C
authors.lastname: Dong
authors.affiliation: East Hospital, Tongji University School of Medicine, Shanghai, China.
authors.forename: Lixin
authors.initials: L
authors.lastname: Sun
authors.affiliation: East Hospital, Tongji University School of Medicine, Shanghai, China.
authors.forename: Liang
authors.initials: L
authors.lastname: Zhu
authors.affiliation: East Hospital, Tongji University School of Medicine, Shanghai, China.
authors.forename: Chenxi
authors.initials: C
authors.lastname: Sun
authors.affiliation: East Hospital, Tongji University School of Medicine, Shanghai, China.
authors.forename: Rongjie
authors.initials: R
authors.lastname: Ma
authors.affiliation: East Hospital, Tongji University School of Medicine, Shanghai, China.|Department of Neuroscience, Sheffield Institute for Translational Neuroscience (SITraN), University of Sheffield, Sheffield, UK.
authors.forename: Ke
authors.initials: K
authors.lastname: Ning
authors.affiliation: East Hospital, Tongji University School of Medicine, Shanghai, China.
authors.forename: Bing
authors.initials: B
authors.lastname: Lu
authors.affiliation: Department of Urology, Shanghai Tongren Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
authors.forename: Jinfu
authors.initials: J
authors.lastname: Zhang
authors.affiliation: East Hospital, Tongji University School of Medicine, Shanghai, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Xu
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000235:genetics; D049109:Cell Proliferation / Q000235:genetics; D005786:Gene Expression Regulation; D006801:Humans; D020547:Lateral Ventricles / Q000378:metabolism / Q000473:pathology; D051379:Mice; D009419:Nerve Tissue Proteins / Q000235:genetics*; D058953:Neural Stem Cells / Q000378:metabolism / Q000473:pathology; D019636:Neurodegenerative Diseases / Q000235:genetics* / Q000473:pathology; D055495:Neurogenesis / Q000235:genetics*; D040901:Proteomics; D055153:Stem Cell Niche
chemical_list: D009419:Nerve Tissue Proteins