title: Study on the relationship of genotoxic and oxidative potential of a new mixed chelate copper antitumoral drug, Casiopeina II-gly (Cas II-gly) in Drosophila melanogaster.
abstract: The present study evaluates the superoxide dismutase (SOD) and catalase (CAT) activities in a wild strain of Drosophila melanogaster and the genotoxic potential induced by Cas II-gly (a new antineoplastic drug) using the somatic mutation and recombination test. Larvae 48h old were treated with Cas II-gly in a range of 0-1.5mM and aliquot were taken every 24h to have individuals treated for 24, 48, 72h and adulthood as well. A dose-dependent toxicity and a significant increase in SOD and CAT activities were found after a 24 and 48h treatment with 0.5-1.5mM concentrations. The comparison of the effect in enzymes with mutation indicated a positive correlation with increased genetic damage, after 24 and 48h of exposure for all concentrations tested. The addition of the genetic damage induced in each exposure time showed a significant effect, but only the small single spots had a concentration-related increase.
authors.affiliation: Departamento de Biología, Instituto Nacional de Investigaciones Nucleares (ININ), Carretera México-Toluca, S/N, la Marquesa, Ocoyoacac, CP. 52750, Mexico.
authors.forename: V E
authors.initials: VE
authors.lastname: Jiménez
authors.affiliation: Departamento de Biología, Instituto Nacional de Investigaciones Nucleares (ININ), Carretera México-Toluca, S/N, la Marquesa, Ocoyoacac, CP. 52750, Mexico. Electronic address: emilio.pimentel@inin.gob.mx.
authors.forename: E
authors.initials: E
authors.lastname: Pimentel
authors.affiliation: Departamento de Biología, Instituto Nacional de Investigaciones Nucleares (ININ), Carretera México-Toluca, S/N, la Marquesa, Ocoyoacac, CP. 52750, Mexico.
authors.forename: M P
authors.initials: MP
authors.lastname: Cruces
authors.affiliation: Facultad de Química, Universidad Autónoma del Estado de México, Toluca, Mexico.
authors.forename: A
authors.initials: A
authors.lastname: Amaya-Chávez
authors.affiliation: Departamento de Química Inorgánica y Nuclear, Facultad de Química, Universidad Nacional Autónoma de México, Avenida Universidad 3000, 04510, Ciudad de México, Mexico.
authors.forename: L
authors.initials: L
authors.lastname: Ruiz-Azuara
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000633:toxicity*; D002374:Catalase / Q000378:metabolism; D002614:Chelating Agents / Q000633:toxicity*; D003300:Copper / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D004331:Drosophila melanogaster / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D005260:Female; D007928:Lethal Dose 50; D015227:Lipid Peroxidation / Q000187:drug effects / Q000235:genetics; D008297:Male; D009153:Mutagens / Q000633:toxicity*; D009942:Organometallic Compounds / Q000633:toxicity*; D018384:Oxidative Stress / Q000187:drug effects* / Q000235:genetics; D011995:Recombination, Genetic / Q000187:drug effects; D013482:Superoxide Dismutase / Q000378:metabolism; D014921:Wings, Animal / Q000187:drug effects
chemical_list: D000970:Antineoplastic Agents; D002614:Chelating Agents; D009153:Mutagens; D009942:Organometallic Compounds; C505569:casiopeina II-glycine; D003300:Copper; D002374:Catalase; D013482:Superoxide Dismutase