title: Repressor logic modules assembled by rolling circle amplification platform to construct a set of logic gates.
abstract: Small molecule metabolites and their allosterically regulated repressors play an important role in many gene expression and metabolic disorder processes. These natural sensors, though valuable as good logic switches, have rarely been employed without transcription machinery in cells. Here, two pairs of repressors, which function in opposite ways, were cloned, purified and used to control DNA replication in rolling circle amplification (RCA) in vitro. By using metabolites and repressors as inputs, RCA signals as outputs, four basic logic modules were constructed successfully. To achieve various logic computations based on these basic modules, we designed series and parallel strategies of circular templates, which can further assemble these repressor modules in an RCA platform to realize twelve two-input Boolean logic gates and a three-input logic gate. The RCA-output and RCA-assembled platform was proved to be easy and flexible for complex logic processes and might have application potential in molecular computing and synthetic biology.
authors.affiliation: Animal Science and Veterinary Medicine College, Shenyang Agricultural University, #120 Dongling Road, Shenyang, Liaoning, 110866, China.|Department of Biochemistry and Molecular Biology, China Medical University, #77 Puhe Road, Shenyang, Liaoning, 110122, China.
authors.forename: Hua
authors.initials: H
authors.lastname: Wei
authors.affiliation: Department of Biochemistry and Molecular Biology, China Medical University, #77 Puhe Road, Shenyang, Liaoning, 110122, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Hu
authors.affiliation: Department of Biochemistry and Molecular Biology, China Medical University, #77 Puhe Road, Shenyang, Liaoning, 110122, China.
authors.forename: Suming
authors.initials: S
authors.lastname: Tang
authors.affiliation: Department of Biochemistry and Molecular Biology, China Medical University, #77 Puhe Road, Shenyang, Liaoning, 110122, China.
authors.forename: Guojie
authors.initials: G
authors.lastname: Zhao
authors.affiliation: Department of Biochemistry and Molecular Biology, China Medical University, #77 Puhe Road, Shenyang, Liaoning, 110122, China.
authors.forename: Yifu
authors.initials: Y
authors.lastname: Guan
mesh_terms: D008128:Logic; D021141:Nucleic Acid Amplification Techniques; D012097:Repressor Proteins / Q000378:metabolism*
chemical_list: D012097:Repressor Proteins