title: Oncolysate-loaded Escherichia coli bacterial ghosts enhance the stimulatory capacity of human dendritic cells.
abstract: The natural adjuvant properties of bacterial ghosts (BGs) lie within the presence of intact pathogen-associated molecular patterns on their surface. BGs can improve the direct delivery, natural processing and presentation of target antigens within dendritic cells (DCs). Moreover, sensitization of human DCs by cancer cell lysate (oncolysate)-loaded BGs in the presence of IFN-α and GM-CSF enhanced DC maturation as indicated by an increased expression of maturation markers and co-stimulatory molecules, higher production of IL-12p70 and stimulation of significantly increased proliferation of both autologous CD4+and CD8+T cells compared to DCs matured in the presence of purified lipopolysaccharide. The induced T cells efficiently recognized oncolysate-derived tumor-associated antigens expressed by cancer cells used for the production of oncolysate. Our optimized one-step simultaneous antigen delivery and DC maturation-inducing method emerges as a promising tool for the development and implementation of next-generation cellular cancer immunotherapies.
authors.affiliation: Cellthera, s.r.o., Brno, Czech Republic.|Advanced Cell Immunotherapy Unit, Department of Pharmacology, Medical Faculty, Masaryk University, Brno, Czech Republic.|Department of Pediatrics, The University Hospital Brno, Brno, Czech Republic.
authors.forename: Jaroslav
authors.initials: J
authors.lastname: Michalek
authors.affiliation: Advanced Cell Immunotherapy Unit, Department of Pharmacology, Medical Faculty, Masaryk University, Brno, Czech Republic.
authors.forename: Renata
authors.initials: R
authors.lastname: Hezova
authors.affiliation: Advanced Cell Immunotherapy Unit, Department of Pharmacology, Medical Faculty, Masaryk University, Brno, Czech Republic.|Department of Pharmacology and Immunotherapy, Veterinary Research Institute, Brno, Czech Republic.
authors.forename: Pavlina
authors.initials: P
authors.lastname: Turanek-Knötigova
authors.affiliation: Institute of Mathematics and Physics, Faculty of Mechanical Engineering, Slovak University of Technology, Bratislava, Slovak Republic.
authors.forename: Jana
authors.initials: J
authors.lastname: Gabkova
authors.affiliation: Department of Immunology, National Cancer Institute, Vilnius, Lithuania.
authors.forename: Marius
authors.initials: M
authors.lastname: Strioga
authors.affiliation: BIRD-C GmbH, Dr. Bohrgasse 2-8, 1030, Vienna, Austria.
authors.forename: Werner
authors.initials: W
authors.lastname: Lubitz
authors.affiliation: BIRD-C GmbH, Dr. Bohrgasse 2-8, 1030, Vienna, Austria. pavol.kudela@bird-c.at.
authors.forename: Pavol
authors.initials: P
authors.lastname: Kudela
mesh_terms: D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology; D002454:Cell Differentiation / Q000276:immunology; D045744:Cell Line, Tumor; D003713:Dendritic Cells / Q000276:immunology* / Q000382:microbiology / Q000637:transplantation; D004926:Escherichia coli / Q000276:immunology*; D005909:Glioblastoma / Q000276:immunology / Q000628:therapy; D006801:Humans; D016219:Immunotherapy, Adoptive / Q000379:methods*; D018664:Interleukin-12 / Q000096:biosynthesis / Q000276:immunology; D008070:Lipopolysaccharides / Q000494:pharmacology; D010641:Phenotype
chemical_list: D008070:Lipopolysaccharides; D018664:Interleukin-12
keywords: Bacterial ghosts; Cancer immunotherapy; Dendritic cells; Natural adjuvant; T cell stimulation; Vaccine