title: Functional analysis of Debaryomyces hansenii Rpn4 on a genetic background of Saccharomyces cerevisiae.
abstract: The transcription factor ScRpn4 coordinates the expression of Saccharomyces cerevisiae proteasomal genes. ScRpn4 orthologues are found in a number of other Saccharomycetes yeasts. Their functions, however, have not yet been characterised experimentally in vivo . We expressed the Debaryomyces hansenii DEHA2D12848 gene encoding an ScRpn4 orthologue (DhRpn4), in an S. cerevisiae strain lacking RPN4 . We showed that DhRpn4 activates transcription of proteasomal genes using ScRpn4 binding site and provides resistance to various stresses. The 43-238 aa segment of DhRpn4 contains an unique portable transactivation domain. Similar to the ScRpn4 N-terminus, this domain lacks a compact structure Moreover, upon overexpression in D. hansenii , DhRpn4 upregulates protesomal genes. Thus, we show that DhRpn4 is the activator for proteasomal genes.
authors.affiliation: Engelhardt Institute of Molecular Biology Russian Academy of Sciences, Department of Intracellular proteolysis regulation, Vavilov str. 32 Moscow 119991, Russia.|Orekhovich Institute of Biomedical Chemistry, Laboratory of Medicinal Proteomics, Pogodinskaya str. 10 Moscow, 119121, Russia.
authors.forename: Dmitry S
authors.initials: DS
authors.lastname: Karpov
authors.affiliation: Engelhardt Institute of Molecular Biology Russian Academy of Sciences, Department of Intracellular proteolysis regulation, Vavilov str. 32 Moscow 119991, Russia.
authors.forename: Evgenia N
authors.initials: EN
authors.lastname: Grineva
authors.affiliation: Engelhardt Institute of Molecular Biology Russian Academy of Sciences, Department of Intracellular proteolysis regulation, Vavilov str. 32 Moscow 119991, Russia.
authors.forename: Arvo T
authors.initials: AT
authors.lastname: Leinsoo
authors.affiliation: Engelhardt Institute of Molecular Biology Russian Academy of Sciences, Department of Intracellular proteolysis regulation, Vavilov str. 32 Moscow 119991, Russia.
authors.forename: Nonna I
authors.initials: NI
authors.lastname: Nadolinskaia
authors.affiliation: Engelhardt Institute of Molecular Biology Russian Academy of Sciences, Department of Intracellular proteolysis regulation, Vavilov str. 32 Moscow 119991, Russia.|Leibniz University of Hanover, Carlomagno Group at Centre of Biomolecular Drug Research, 30159 Hannover, Germany.
authors.forename: Nataliya K
authors.initials: NK
authors.lastname: Danilenko
authors.affiliation: Engelhardt Institute of Molecular Biology Russian Academy of Sciences, Department of Intracellular proteolysis regulation, Vavilov str. 32 Moscow 119991, Russia.
authors.forename: Vera V
authors.initials: VV
authors.lastname: Tutyaeva
authors.affiliation: Engelhardt Institute of Molecular Biology Russian Academy of Sciences, Department of Intracellular proteolysis regulation, Vavilov str. 32 Moscow 119991, Russia.|State Research Institute of Genetics and Selection of Industrial Microorganisms, 1-st Dorozhniy pr., 1, Moscow, 117545, Russia.
authors.forename: Daria S
authors.initials: DS
authors.lastname: Spasskaya
authors.affiliation: Engelhardt Institute of Molecular Biology Russian Academy of Sciences, Department of Intracellular proteolysis regulation, Vavilov str. 32 Moscow 119991, Russia.
authors.forename: Olga V
authors.initials: OV
authors.lastname: Preobrazhenskaya
authors.affiliation: Engelhardt Institute of Molecular Biology Russian Academy of Sciences, Department of Intracellular proteolysis regulation, Vavilov str. 32 Moscow 119991, Russia.
authors.forename: Yuriy P
authors.initials: YP
authors.lastname: Lysov
authors.affiliation: Engelhardt Institute of Molecular Biology Russian Academy of Sciences, Department of Intracellular proteolysis regulation, Vavilov str. 32 Moscow 119991, Russia.
authors.forename: Vadim L
authors.initials: VL
authors.lastname: Karpov
mesh_terms: D015966:Gene Expression Regulation, Fungal; D046988:Proteasome Endopeptidase Complex / Q000235:genetics / Q000378:metabolism*; D011487:Protein Conformation; D000072417:Protein Domains; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000201:enzymology* / Q000235:genetics; D004718:Saccharomycetales / Q000201:enzymology* / Q000235:genetics; D014157:Transcription Factors / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*
chemical_list: D011994:Recombinant Proteins; D014157:Transcription Factors; D046988:Proteasome Endopeptidase Complex
keywords: Debaryomyces hansenii Rpn4; proteasomal genes; transcription regulation