title: Use of Xenopus laevis Oocytes to Study Auxin Transport.
abstract: Xenopus laevis oocytes are an expression system that is particularly well suited for the characterization of membrane transporters. Oocytes possess only very little endogenous transport systems and therefore transporters can be studied with a high signal-to-noise ratio. This book chapter provides the basic methods to use Xenopus oocytes for the characterization of transporters by radiotracer experiments. While the methods described here were established to study auxin transport they can easily be adapted to study other hormone transporters and their substrates.
authors.affiliation: Cell Biology and Plant Biochemistry, University of Regensburg, Universitätsstraße 31, 93053, Regensburg, Germany.
authors.forename: Astrid
authors.initials: A
authors.lastname: Fastner
authors.affiliation: Cell Biology and Plant Biochemistry, University of Regensburg, Universitätsstraße 31, 93053, Regensburg, Germany.
authors.forename: Birgit
authors.initials: B
authors.lastname: Absmanner
authors.affiliation: Cell Biology and Plant Biochemistry, University of Regensburg, Universitätsstraße 31, 93053, Regensburg, Germany. ulrich.hammes@biologie.uni-regensburg.de.
authors.forename: Ulrich Z
authors.initials: UZ
authors.lastname: Hammes
mesh_terms: D000818:Animals; D001692:Biological Transport / Q000502:physiology*; D007210:Indoleacetic Acids / Q000378:metabolism*; D026901:Membrane Transport Proteins / Q000378:metabolism*; D009865:Oocytes / Q000378:metabolism*; D010937:Plant Growth Regulators / Q000378:metabolism*; D059629:Signal-To-Noise Ratio; D014982:Xenopus laevis / Q000378:metabolism*
chemical_list: D007210:Indoleacetic Acids; D026901:Membrane Transport Proteins; D010937:Plant Growth Regulators
keywords: Import export; Liquid scintillation; Microinjection; Radiotracer; Transport; Xenopus laevis oocytes