title: A Transcriptional Switch Point During Hematopoietic Stem and Progenitor Cell Ontogeny.
abstract: During mammalian embryogenesis, hematopoietic stem and progenitor cells (HSPCs) originate from mesoderm-derived endothelial cells in the aorta-gonad-mesonephros (AGM) region and placenta (PL). Later, HSPCs expand in fetal liver (FL) and migrate to bone marrow (BM) shortly before birth. Understanding global transcriptional regulation governing HSPC emergence from embryonic stem/induced pluripotent stem cells is necessary to devise clinical applications, such as novel transplantation approaches. In this study, to assess transcriptional dynamics during development, we performed cap analysis of gene expression on 10 developmental murine HSPC populations isolated from the AGM region, PL, FL, and BM and identified 15,681 transcripts across HSPC ontogeny. We performed microarray analysis of AGM-derived HSPCs at 9.5 and 10.5 days postcoitum (dpc) and identified 40 differentially expressed genes, 23 confirmed as significantly changed by real-time polymerase chain reaction. We conclude that a transcriptional switch point occurs in HSPC ontogeny between 9.5 and 10.5 dpc in the AGM region.
authors.affiliation: 1 Department of Research and Development of Next Generation Medicine, Faculty of Medical Sciences, Kyushu University , Fukuoka, Japan .|2 Center for Clinical and Translational Research, Kyushu University , Fukuoka, Japan .|3 Department of Clinical Study, Center for Advanced Medical Innovation, Kyushu University , Fukuoka, Japan .
authors.forename: Daisuke
authors.initials: D
authors.lastname: Sugiyama
authors.affiliation: 4 The Roslin Institute and Royal (Dick) School of Veterinary Studies, University of Edinburgh , Easter Bush Campus, Midlothian, United Kingdom .
authors.forename: Anagha
authors.initials: A
authors.lastname: Joshi
authors.affiliation: 1 Department of Research and Development of Next Generation Medicine, Faculty of Medical Sciences, Kyushu University , Fukuoka, Japan .
authors.forename: Kasem
authors.initials: K
authors.lastname: Kulkeaw
authors.affiliation: 1 Department of Research and Development of Next Generation Medicine, Faculty of Medical Sciences, Kyushu University , Fukuoka, Japan .
authors.forename: Keai Sinn
authors.initials: KS
authors.lastname: Tan
authors.affiliation: 1 Department of Research and Development of Next Generation Medicine, Faculty of Medical Sciences, Kyushu University , Fukuoka, Japan .
authors.forename: Tomoko
authors.initials: T
authors.lastname: Yokoo-Inoue
authors.affiliation: 1 Department of Research and Development of Next Generation Medicine, Faculty of Medical Sciences, Kyushu University , Fukuoka, Japan .
authors.forename: Chiyo
authors.initials: C
authors.lastname: Mizuochi-Yanagi
authors.affiliation: 5 Cell Innovator Ltd. , Fukuoka, Japan .
authors.forename: Kaori
authors.initials: K
authors.lastname: Yasuda
authors.affiliation: 5 Cell Innovator Ltd. , Fukuoka, Japan .
authors.forename: Atsushi
authors.initials: A
authors.lastname: Doi
authors.affiliation: 3 Department of Clinical Study, Center for Advanced Medical Innovation, Kyushu University , Fukuoka, Japan .
authors.forename: Tadafumi
authors.initials: T
authors.lastname: Iino
authors.affiliation: 6 RIKEN Preventive Medicine and Diagnosis Innovation Program , Yokohama, Japan .|7 RIKEN Center for Life Science Technologies , Division of Genomic Technologies, Yokohama, Japan .|8 RIKEN Yokohama Institute , Omics Science Center, Yokohama, Kanagawa, Japan.
authors.forename: Masayoshi
authors.initials: M
authors.lastname: Itoh
authors.affiliation: 8 RIKEN Yokohama Institute , Omics Science Center, Yokohama, Kanagawa, Japan.
authors.forename: Sayaka
authors.initials: S
authors.lastname: Nagao-Sato
authors.affiliation: 9 Division of Molecular and Clinical Genetics, Medical Institute of Bioregulation, Kyushu University , Fukuoka, Japan .
authors.forename: Kenzaburo
authors.initials: K
authors.lastname: Tani
authors.affiliation: 10 Department of Medicine and Biosystemic Science, Kyushu University Graduate School of Medical Sciences , Fukuoka, Japan .
authors.forename: Koichi
authors.initials: K
authors.lastname: Akashi
authors.affiliation: 6 RIKEN Preventive Medicine and Diagnosis Innovation Program , Yokohama, Japan .|7 RIKEN Center for Life Science Technologies , Division of Genomic Technologies, Yokohama, Japan .
authors.forename: Yoshihide
authors.initials: Y
authors.lastname: Hayashizaki
authors.affiliation: 7 RIKEN Center for Life Science Technologies , Division of Genomic Technologies, Yokohama, Japan .
authors.forename: Harukazu
authors.initials: H
authors.lastname: Suzuki
authors.affiliation: 6 RIKEN Preventive Medicine and Diagnosis Innovation Program , Yokohama, Japan .|7 RIKEN Center for Life Science Technologies , Division of Genomic Technologies, Yokohama, Japan .|8 RIKEN Yokohama Institute , Omics Science Center, Yokohama, Kanagawa, Japan.
authors.forename: Hideya
authors.initials: H
authors.lastname: Kawaji
authors.affiliation: 6 RIKEN Preventive Medicine and Diagnosis Innovation Program , Yokohama, Japan .|7 RIKEN Center for Life Science Technologies , Division of Genomic Technologies, Yokohama, Japan .|8 RIKEN Yokohama Institute , Omics Science Center, Yokohama, Kanagawa, Japan.
authors.forename: Piero
authors.initials: P
authors.lastname: Carninci
authors.affiliation: 7 RIKEN Center for Life Science Technologies , Division of Genomic Technologies, Yokohama, Japan .
authors.forename: Alistair R R
authors.initials: AR
authors.lastname: Forrest
mesh_terms: D000818:Animals; D001011:Aorta / Q000166:cytology; D002469:Cell Separation; D004622:Embryo, Mammalian / Q000166:cytology / Q000378:metabolism; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D006066:Gonads / Q000166:cytology; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000378:metabolism*; D008650:Mesonephros / Q000166:cytology; D008810:Mice, Inbred C57BL; D008813:Mice, Inbred ICR; D020411:Oligonucleotide Array Sequence Analysis; D012315:RNA Caps / Q000378:metabolism; D015203:Reproducibility of Results; D014158:Transcription, Genetic
chemical_list: D012315:RNA Caps
keywords: cap analysis of gene expression; hematopoietic stem and progenitor cells; ontogeny