title: Identification and Expression Analysis of Polygalacturonase Family Members during Peach Fruit Softening.
abstract: Polygalacturonase (PG) is an important hydrolytic enzyme involved in pectin degradation during fruit softening. However, the roles ofPGfamily members in fruit softening remain unclear. We identified 45PpPGgenes in the peach genome which are clustered into six subclasses.PpPGsconsist of four to nine exons and three to eight introns, and the exon/intron structure is basically conserved in all but subclass E. Only 16PpPGgenes were expressed in ripening fruit, and their expression profiles were analyzed during storage in two peach cultivars with different softening characteristics. EightPGs(PpPG1, -10, -12, -13, -15, -23, -21, and -22) in fast-softening "Qian Jian Bai" (QJB) fruit and threePGs(PpPG15, -21, and -22) in slow-softening "Qin Wang" (QW) fruit exhibited softening-associated patterns; which also were affected by ethylene treatment. Our results suggest that the different softening characters in QW and QJB fruit is related to the amount ofPGmembers. While keeping relatively lower levels during QW fruit softening, the expression of sixPGs(PpPG1, -10, -12, -11, -14, and -35) rapidly induced by ethylene.PpPG24, -25and -38may not be involved in softening of peach fruit.
authors.affiliation: College of Horticulture, Northwest A&F University, Yangling 712100, China. m13637099856@163.com.
authors.forename: Ming
authors.initials: M
authors.lastname: Qian
authors.affiliation: College of Horticulture, Northwest A&F University, Yangling 712100, China. 18821635518@163.com.
authors.forename: Yike
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: College of Horticulture, Northwest A&F University, Yangling 712100, China. xiangyan0209@163.com.
authors.forename: Xiangyan
authors.initials: X
authors.lastname: Yan
authors.affiliation: College of Horticulture, Northwest A&F University, Yangling 712100, China. hanmy@nwsuaf.edu.cn.
authors.forename: Mingyu
authors.initials: M
authors.lastname: Han
authors.affiliation: College of Horticulture, Northwest A&F University, Yangling 712100, China. lijinjin@bigizy.cn.
authors.forename: Jinjin
authors.initials: J
authors.lastname: Li
authors.affiliation: College of Horticulture, Northwest A&F University, Yangling 712100, China. fangfang0918@126.com.
authors.forename: Fang
authors.initials: F
authors.lastname: Li
authors.affiliation: College of Horticulture, Northwest A&F University, Yangling 712100, China. 18821635911@163.com.
authors.forename: Furui
authors.initials: F
authors.lastname: Li
authors.affiliation: College of Horticulture, Northwest A&F University, Yangling 712100, China. afant@nwsuaf.edu.cn.
authors.forename: Dong
authors.initials: D
authors.lastname: Zhang
authors.affiliation: College of Horticulture, Northwest A&F University, Yangling 712100, China. zhcc@nwsuaf.edu.cn.
authors.forename: Caiping
authors.initials: C
authors.lastname: Zhao
mesh_terms: D001483:Base Sequence; D002874:Chromosome Mapping; D032461:Chromosomes, Plant / Q000235:genetics; D005030:Ethylenes / Q000096:biosynthesis; D005638:Fruit / Q000235:genetics* / Q000378:metabolism; D020869:Gene Expression Profiling / Q000379:methods; D015971:Gene Expression Regulation, Enzymologic; D018506:Gene Expression Regulation, Plant; D007527:Isoenzymes / Q000145:classification / Q000235:genetics / Q000378:metabolism; D005810:Multigene Family; D010802:Phylogeny; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D011096:Polygalacturonase / Q000145:classification / Q000235:genetics* / Q000378:metabolism; D000068239:Prunus persica / Q000145:classification / Q000201:enzymology / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity
chemical_list: D005030:Ethylenes; D007527:Isoenzymes; D010940:Plant Proteins; C036216:ethylene; D011096:Polygalacturonase
keywords: PG family; fruit; peach; ripening; softening