title: Exploiting NanoLuc luciferase for smartphone-based bioluminescence cell biosensor for (anti)-inflammatory activity and toxicity.
abstract: The availability of smartphones with high-performance digital image sensors and processing power has completely reshaped the landscape of point-of-need analysis. Thanks to the high maturity level of reporter gene technology and the availability of several bioluminescent proteins with improved features, we were able to develop a bioluminescence smartphone-based biosensing platform exploiting the highly sensitive NanoLuc luciferase as reporter. A 3D-printed smartphone-integrated cell biosensor based on genetically engineered Hek293T cells was developed. Quantitative assessment of (anti)-inflammatory activity and toxicity of liquid samples was performed with a simple and rapid add-and-measure procedure. White grape pomace extracts, known to contain several bioactive compounds, were analyzed, confirming the suitability of the smartphone biosensing platform for analysis of untreated complex biological matrices. Such approach could meet the needs of small medium enterprises lacking fully equipped laboratories for first-level safety tests and rapid screening of new bioactive products. Graphical abstract Smartphone-based bioluminescence cell biosensor.
authors.affiliation: Department of Chemistry "G. Ciamician", University of Bologna, Via Selmi 2, 40126, Bologna, Italy.
authors.forename: Luca
authors.initials: L
authors.lastname: Cevenini
authors.affiliation: Department of Chemistry "G. Ciamician", University of Bologna, Via Selmi 2, 40126, Bologna, Italy.
authors.forename: Maria Maddalena
authors.initials: MM
authors.lastname: Calabretta
authors.affiliation: Department of Chemistry "G. Ciamician", University of Bologna, Via Selmi 2, 40126, Bologna, Italy.
authors.forename: Antonia
authors.initials: A
authors.lastname: Lopreside
authors.affiliation: Department of Chemistry "G. Ciamician", University of Bologna, Via Selmi 2, 40126, Bologna, Italy.|Centro Interdipartimentale di Ricerche sul Cancro "Giorgio Prodi" (CIRC), Bologna, Italy.
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Tarantino
authors.affiliation: Department of Biological, Geological, and Environmental Sciences, University of Bologna, via Irnerio 42, 40126, Bologna, Italy.
authors.forename: Annalisa
authors.initials: A
authors.lastname: Tassoni
authors.affiliation: Department of Biological, Geological, and Environmental Sciences, University of Bologna, via Irnerio 42, 40126, Bologna, Italy.
authors.forename: Maura
authors.initials: M
authors.lastname: Ferri
authors.affiliation: Department of Chemistry "G. Ciamician", University of Bologna, Via Selmi 2, 40126, Bologna, Italy. aldo.roda@unibo.it.|INBB, Istituto Nazionale di Biostrutture e Biosistemi, Viale delle Medaglie d'Oro 305, 00136, Rome, Italy. aldo.roda@unibo.it.
authors.forename: Aldo
authors.initials: A
authors.lastname: Roda
authors.affiliation: Department of Chemistry "G. Ciamician", University of Bologna, Via Selmi 2, 40126, Bologna, Italy. elisa.michelini8@unibo.it.|INBB, Istituto Nazionale di Biostrutture e Biosistemi, Viale delle Medaglie d'Oro 305, 00136, Rome, Italy. elisa.michelini8@unibo.it.
authors.forename: Elisa
authors.initials: E
authors.lastname: Michelini
mesh_terms: D000893:Anti-Inflammatory Agents / Q000737:chemistry / Q000494:pharmacology*; D015374:Biosensing Techniques / Q000295:instrumentation*; D004867:Equipment Design; D017930:Genes, Reporter; D005818:Genetic Engineering; D057809:HEK293 Cells; D006801:Humans; D057230:Limit of Detection; D008156:Luciferases / Q000235:genetics* / Q000378:metabolism; D008163:Luminescent Measurements / Q000295:instrumentation*; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D015203:Reproducibility of Results; D000068997:Smartphone / Q000295:instrumentation*; D027843:Vitis / Q000737:chemistry
chemical_list: D000893:Anti-Inflammatory Agents; D010936:Plant Extracts; D008156:Luciferases
keywords: Anti-inflammatory activity; Bioluminescence; Cell biosensor; Portable analytical device; Smartphone; Toxicity