title: Acute phase protein haptoglobin as inflammatory marker in serum and synovial fluid in an equine model of arthritis.
abstract: Acute phase proteins are useful inflammatory markers in horses. Haptoglobin (Hp) serum level is increased in horses undergoing different inflammatory processes, including arthritis. However, Hp concentration has not been assessed in inflammatory synovial fluid (SF). The aim of the present study was to investigate the Hp response in serum and SF in horses undergoing experimentally induced arthritis. For this purpose, serum and SF samples were collected from 12 animals before amphotericin B-induced arthritis was created (T0, healthy) and 15days after the lesion induction (T1, joint inflammation) and Hp was determined by single radial immunodiffusion. The Hp increase between T0 and T1 was significant in both serum and SF, and serum Hp concentration at T0 was significantly higher than in SF, but significant differences were not found at T1, indicating a higher Hp increase in SF. A significant positive correlation for Hp concentration between serum and SF samples was found. These results highlight the potential usefulness of Hp as inflammatory marker in horses, showing for the first time the increase of Hp in SF from joint inflammation in the horse.
authors.affiliation: Laboratorio de Genética Bioquímica LAGENBIO (Universidad de Zaragoza), Instituto Agroalimentario de Aragón- IA2 - (Universidad de Zaragoza-CITA), Instituto de Investigación Sanitaria de Aragón (IIS), C/Miguel Servet, 177, 50013 Zaragoza, Spain; Servicio de Cirugía y Medicina Equina, Hospital Veterinario, Universidad de Zaragoza, C/Miguel Servet, 177, 50013 Zaragoza, Spain.
authors.forename: Laura
authors.initials: L
authors.lastname: Barrachina
authors.affiliation: Laboratorio de Genética Bioquímica LAGENBIO (Universidad de Zaragoza), Instituto Agroalimentario de Aragón- IA2 - (Universidad de Zaragoza-CITA), Instituto de Investigación Sanitaria de Aragón (IIS), C/Miguel Servet, 177, 50013 Zaragoza, Spain.
authors.forename: Ana Rosa
authors.initials: AR
authors.lastname: Remacha
authors.affiliation: Departamento de Bioquímica y Biología Molecular y Celular, Facultad de Ciencias, Universidad de Zaragoza, C/Pedro Cerbuna, 12, 50009, Zaragoza, Spain.
authors.forename: Lourdes
authors.initials: L
authors.lastname: Soler
authors.affiliation: Departamento de Bioquímica y Biología Molecular y Celular, Facultad de Ciencias, Universidad de Zaragoza, C/Pedro Cerbuna, 12, 50009, Zaragoza, Spain.
authors.forename: Natalia
authors.initials: N
authors.lastname: García
authors.affiliation: Laboratorio de Genética Bioquímica LAGENBIO (Universidad de Zaragoza), Instituto Agroalimentario de Aragón- IA2 - (Universidad de Zaragoza-CITA), Instituto de Investigación Sanitaria de Aragón (IIS), C/Miguel Servet, 177, 50013 Zaragoza, Spain; Servicio de Cirugía y Medicina Equina, Hospital Veterinario, Universidad de Zaragoza, C/Miguel Servet, 177, 50013 Zaragoza, Spain.
authors.forename: Antonio
authors.initials: A
authors.lastname: Romero
authors.affiliation: Laboratorio de Genética Bioquímica LAGENBIO (Universidad de Zaragoza), Instituto Agroalimentario de Aragón- IA2 - (Universidad de Zaragoza-CITA), Instituto de Investigación Sanitaria de Aragón (IIS), C/Miguel Servet, 177, 50013 Zaragoza, Spain; Servicio de Cirugía y Medicina Equina, Hospital Veterinario, Universidad de Zaragoza, C/Miguel Servet, 177, 50013 Zaragoza, Spain.
authors.forename: Francisco José
authors.initials: FJ
authors.lastname: Vázquez
authors.affiliation: Laboratorio de Genética Bioquímica LAGENBIO (Universidad de Zaragoza), Instituto Agroalimentario de Aragón- IA2 - (Universidad de Zaragoza-CITA), Instituto de Investigación Sanitaria de Aragón (IIS), C/Miguel Servet, 177, 50013 Zaragoza, Spain; Servicio de Cirugía y Medicina Equina, Hospital Veterinario, Universidad de Zaragoza, C/Miguel Servet, 177, 50013 Zaragoza, Spain.
authors.forename: Arantza
authors.initials: A
authors.lastname: Vitoria
authors.affiliation: Departamento de Bioquímica y Biología Molecular y Celular, Facultad de Ciencias, Universidad de Zaragoza, C/Pedro Cerbuna, 12, 50009, Zaragoza, Spain.
authors.forename: María Ángeles
authors.initials: MÁ
authors.lastname: Álava
authors.affiliation: Departamento de Bioquímica y Biología Molecular y Celular, Facultad de Ciencias, Universidad de Zaragoza, C/Pedro Cerbuna, 12, 50009, Zaragoza, Spain.
authors.forename: Fermín
authors.initials: F
authors.lastname: Lamprave
authors.affiliation: Laboratorio de Genética Bioquímica LAGENBIO (Universidad de Zaragoza), Instituto Agroalimentario de Aragón- IA2 - (Universidad de Zaragoza-CITA), Instituto de Investigación Sanitaria de Aragón (IIS), C/Miguel Servet, 177, 50013 Zaragoza, Spain. Electronic address: rodellar@unizar.es.
authors.forename: Clementina
authors.initials: C
authors.lastname: Rodellar
mesh_terms: D000818:Animals; D001168:Arthritis / Q000097:blood / Q000378:metabolism / Q000662:veterinary*; D001169:Arthritis, Experimental / Q000097:blood / Q000378:metabolism; D015415:Biomarkers / Q000097:blood / Q000378:metabolism; D006242:Haptoglobins / Q000378:metabolism*; D006734:Horse Diseases / Q000097:blood* / Q000378:metabolism*; D006736:Horses; D018836:Inflammation Mediators / Q000097:blood / Q000378:metabolism; D008297:Male; D013582:Synovial Fluid / Q000378:metabolism; D013997:Time Factors
chemical_list: D015415:Biomarkers; D006242:Haptoglobins; D018836:Inflammation Mediators
keywords: Acute phase protein; Equine arthritis; Equine haptoglobin; Serum; Synovial fluid