title: The timeline of corona formation around silica nanocarriers highlights the role of the protein interactome.
abstract: Magnetic mesoporous silica nanoparticles (M-MSNs) represent promising targeting tools for theranostics. Engineering the interaction of nanoparticles (NPs) with biological systems requires an understanding of protein corona formation around the nanoparticles as this drives the biological fate of nanocarriers. We investigated the behavior of proteins in contact with M-MSNs by high-throughput comparative proteomics, using human and bovine sera as biological fluids, in order to assess the adsorption dynamics of proteins in these media. Using system biology tools, and especially protein-protein interaction databases, we demonstrated how the protein network builds up within the corona over the course of the experiment. Based on these results, we introduce and discuss the role of the "corona interactome" as an important factor influencing protein corona evolution. The concept of the "corona interactome" is an original methodology which could be generalized to all NP candidates. Based on this, pre-coating nanocarriers with specific proteins presenting minimal interactions with opsonins might provide them with properties such as stealth.
authors.affiliation: Institut Charles Gerhardt de Montpellier, MACS, UMR 5253 CNRS-ENSCM-UM, 1701, Place Eugène Bataillon, F-34095 Montpellier, France and CEA, Direction de la Recherche Fondamentale-BIAM, Site de Marcoule, F-30207 Bagnols-sur-Cèze, France. odette.prat@cea.fr.
authors.forename: Cédric
authors.initials: C
authors.lastname: Pisani
authors.affiliation: CEA, Direction de la Recherche Fondamentale-IBITECS, Site de Marcoule, F-30207 Bagnols-sur-Cèze, France.
authors.forename: Jean-Charles
authors.initials: JC
authors.lastname: Gaillard
authors.affiliation: Institut de Chimie Séparative de Marcoule, UMR 5257 CEA-CNRS-ENSCM-UM, Site de Marcoule, F-30207 Bagnols-sur-Cèze, France.
authors.forename: Michaël
authors.initials: M
authors.lastname: Odorico
authors.affiliation: Institut Charles Gerhardt de Montpellier, IMNO, UMR 5253 CNRS-ENSCM-UM, 1701, Place Eugène Bataillon, F-34095 Montpellier, France.
authors.forename: Jeff L
authors.initials: JL
authors.lastname: Nyalosaso
authors.affiliation: Institut Charles Gerhardt de Montpellier, IMNO, UMR 5253 CNRS-ENSCM-UM, 1701, Place Eugène Bataillon, F-34095 Montpellier, France.
authors.forename: Clarence
authors.initials: C
authors.lastname: Charnay
authors.affiliation: Institut Charles Gerhardt de Montpellier, IMNO, UMR 5253 CNRS-ENSCM-UM, 1701, Place Eugène Bataillon, F-34095 Montpellier, France.
authors.forename: Yannick
authors.initials: Y
authors.lastname: Guari
authors.affiliation: Institut Charles Gerhardt de Montpellier, MACS, UMR 5253 CNRS-ENSCM-UM, 1701, Place Eugène Bataillon, F-34095 Montpellier, France and Université de Nîmes Rue Georges Salan, F-30000 Nîmes, France.
authors.forename: Joël
authors.initials: J
authors.lastname: Chopineau
authors.affiliation: Institut Charles Gerhardt de Montpellier, MACS, UMR 5253 CNRS-ENSCM-UM, 1701, Place Eugène Bataillon, F-34095 Montpellier, France.
authors.forename: Jean-Marie
authors.initials: JM
authors.lastname: Devoisselle
authors.affiliation: CEA, Direction de la Recherche Fondamentale-IBITECS, Site de Marcoule, F-30207 Bagnols-sur-Cèze, France.
authors.forename: Jean
authors.initials: J
authors.lastname: Armengaud
authors.affiliation: CEA, Direction de la Recherche Fondamentale-BIAM, Site de Marcoule, F-30207 Bagnols-sur-Cèze, France. odette.prat@cea.fr.
authors.forename: Odette
authors.initials: O
authors.lastname: Prat
mesh_terms: D000327:Adsorption; D000818:Animals; D002417:Cattle; D006801:Humans; D053758:Nanoparticles; D000066970:Protein Corona / Q000737:chemistry*; D060066:Protein Interaction Maps; D044967:Serum; D012822:Silicon Dioxide; D049490:Systems Biology
chemical_list: D000066970:Protein Corona; D012822:Silicon Dioxide