title: Brain-Derived Neurotrophic Factor Inhibits Peptidoglycan-Induced Inflammatory Cytokine Expression in Human Dental Pulp Cells.
abstract: The aim of this study was to examine the anti-inflammatory effect of brain-derived neurotrophic factor (BDNF) on human dental pulp cells (HDPCs) and identify the intracellular signaling pathway involved. We investigated the effect of BDNF (50 ng/ml) on interleukin (IL)-6 and IL-8 expression in peptidoglycan (PGN)-treated HDPCs. An inhibition assay was performed with MAPK or NF-κB inhibitors to determine the possible signaling pathway. IL-6 and IL-8 mRNA, IL-6 and IL-8 protein, and phosphorylated p38 kinase activity were determined using real-time PCR, ELISA, and Western blot analysis, respectively. BDNF significantly attenuated PGN-induced IL-6 and IL-8 mRNA and protein levels in HDPCs. A p38 inhibitor also inhibited IL-6 and IL-8 mRNA transcription. PGN stimulated phosphorylated p38 kinase activity in HDPCs, which was inhibited by BDNF. Suppression of phosphorylated p38 kinase activity by BDNF in HDPCs inhibited increased IL-6 and IL-8 expression induced by PGN. Our findings suggest that BDNF regulates intracellular signaling molecule activities to exert its anti-inflammatory effect.
authors.affiliation: Department of Periodontal Medicine, Division of Applied Life Sciences, Institute of Biomedical and Health Sciences, Hiroshima University, Hiroshima, Japan. takepon@hiroshima-u.ac.jp.
authors.forename: Katsuhiro
authors.initials: K
authors.lastname: Takeda
authors.affiliation: Department of Periodontal Medicine, Division of Applied Life Sciences, Institute of Biomedical and Health Sciences, Hiroshima University, Hiroshima, Japan.
authors.forename: Naoko
authors.initials: N
authors.lastname: Tokunaga
authors.affiliation: Department of Periodontal Medicine, Division of Applied Life Sciences, Institute of Biomedical and Health Sciences, Hiroshima University, Hiroshima, Japan.
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Aida
authors.affiliation: Department of Periodontal Medicine, Division of Applied Life Sciences, Institute of Biomedical and Health Sciences, Hiroshima University, Hiroshima, Japan.
authors.forename: Mikihito
authors.initials: M
authors.lastname: Kajiya
authors.affiliation: Department of Periodontal Medicine, Division of Applied Life Sciences, Institute of Biomedical and Health Sciences, Hiroshima University, Hiroshima, Japan.
authors.forename: Kazuhisa
authors.initials: K
authors.lastname: Ouhara
authors.affiliation: Department of Periodontal Medicine, Division of Applied Life Sciences, Institute of Biomedical and Health Sciences, Hiroshima University, Hiroshima, Japan.
authors.forename: Shinya
authors.initials: S
authors.lastname: Sasaki
authors.affiliation: Department of Periodontal Medicine, Division of Applied Life Sciences, Institute of Biomedical and Health Sciences, Hiroshima University, Hiroshima, Japan.
authors.forename: Noriyoshi
authors.initials: N
authors.lastname: Mizuno
authors.affiliation: Department of Periodontal Medicine, Division of Applied Life Sciences, Institute of Biomedical and Health Sciences, Hiroshima University, Hiroshima, Japan.
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Fujita
authors.affiliation: Department of Periodontal Medicine, Division of Applied Life Sciences, Institute of Biomedical and Health Sciences, Hiroshima University, Hiroshima, Japan.
authors.forename: Hidemi
authors.initials: H
authors.lastname: Kurihara
mesh_terms: D019208:Brain-Derived Neurotrophic Factor / Q000502:physiology*; D016207:Cytokines / Q000378:metabolism*; D003782:Dental Pulp / Q000166:cytology*; D006801:Humans; D007249:Inflammation; D018836:Inflammation Mediators / Q000378:metabolism; D015850:Interleukin-6 / Q000378:metabolism; D016209:Interleukin-8 / Q000378:metabolism; D010457:Peptidoglycan / Q000494:pharmacology*; D015398:Signal Transduction; D048051:p38 Mitogen-Activated Protein Kinases / Q000378:metabolism
chemical_list: D019208:Brain-Derived Neurotrophic Factor; D016207:Cytokines; D018836:Inflammation Mediators; D015850:Interleukin-6; D016209:Interleukin-8; D010457:Peptidoglycan; C579455:BDNF protein, human; D048051:p38 Mitogen-Activated Protein Kinases
keywords: brain-derived neurotrophic factor; endodontic treatment; peptidoglycan; pulp cells