title: Bioanalytical method for the estimation of co-administered esomeprazole, leflunomide and ibuprofen in human plasma and in pharmaceutical dosage forms using micellar liquid chromatography.
abstract: The present study represents a connection between basic science and clinical applied science through providing a bioanalytical method for the analysis of certain co-administered drugs used for the treatment of rheumatoid arthritis. The studied drugs are esomeprazole, leflunomide and ibuprofen. The proposed bioanalytical method is a simple reversed phase high performance liquid chromatographic method using micellar mobile phase. The method is conducted using a Shim-pack VP-ODS (150 mm × 4.6 mm ID) stainless steel column at ambient temperature with ultraviolet detection at 285 nm. The micellar mobile phase consisted of 0.1 m sodium dodecyl sulfate, 10% n-propanol, 0.3% triethylamine in 0.02 m orthophosphoric acid (pH 3.5) and is pumped at a flow rate of 1.0 mL/min. The calibration curve was rectilinear over the concentration range of 0.1-5.0, 0.5-10.0 and 1.0-20.0 μg/mL for esomeprazole, leflunomide and ibuprofen respectively. The proposed method was successfully applied to the analysis of these drugs in dosage forms. The method is extended to the in-vitro, in-vivo determination of these drugs in spiked and real human plasma samples.
authors.affiliation: Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, Damanhour University, Damnhour, Egypt.
authors.forename: Wael
authors.initials: W
authors.lastname: Talaat
mesh_terms: D000328:Adult; D002138:Calibration; D002853:Chromatography, Liquid / Q000379:methods*; D004359:Drug Therapy, Combination; D064098:Esomeprazole / Q000008:administration & dosage / Q000097:blood*; D005021:Ethylamines / Q000737:chemistry; D006801:Humans; D007052:Ibuprofen / Q000008:administration & dosage / Q000097:blood*; D007555:Isoxazoles / Q000008:administration & dosage / Q000097:blood*; D000077339:Leflunomide; D057230:Limit of Detection; D008297:Male; D008823:Micelles; D010756:Phosphoric Acids / Q000737:chemistry; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012967:Sodium Dodecyl Sulfate / Q000737:chemistry; D014466:Ultraviolet Rays
chemical_list: D005021:Ethylamines; D007555:Isoxazoles; D008823:Micelles; D010756:Phosphoric Acids; D012967:Sodium Dodecyl Sulfate; C030242:phosphoric acid; D000077339:Leflunomide; D064098:Esomeprazole; C016162:triethylamine; D007052:Ibuprofen
keywords: Bioanalysis; Biological fluids; HPLC; Micellar mobile phase