title: A STUDY OF THE RELATIONSHIP BETWEEN LEVELS OF METHYLTRANSFERASES IN PERIPHERAL BLOOD MONONUCLEAR CELLS AND CHARACTERISTICS OF TUMOR IN PATIENTS WITH DUCTAL INVASIVE CARCINOMA OF BREAST.
abstract: The role of epigenetics in tumor development and progression is actively being studied. The aim of our current pilot study is to analyze correlation of changes in the levels of methyltransferases in nuclear extracts of blood cells with some morphological and phenotypic characteristics of breast cancer. The levels of DNMT1, DNMT3a and H3K4 methyltransferase were measured. The results showed that the level of DNMT1 was highest in the control group but correlation with the tumor grade was just moderate. DNMT3a was found in highest level in Grade III cancer group, followed by Grade II and Grade I groups. Correlation of DNMT1 level with tumor grade was moderate. An opposite pattern was seen for H3K4 methyltransferase. DNMT3a level was higher in larger tumors, while the level of H3K4 methyltransferase was lowest in large tumors with significant negative correlation with the tumor size. This primary study shows that there are some changes in methyltransferase levels in PBMC from breast cancer patients. These changes are most probably attributed to modification of initiation as well as sustainment of methylated status of products.
authors.affiliation: Tbilisi State Medical University, Department of Molecular and Medical Genetics, Georgia.
authors.forename: K
authors.initials: K
authors.lastname: Kankava
authors.affiliation: Tbilisi State Medical University, Department of Molecular and Medical Genetics, Georgia.
authors.forename: E
authors.initials: E
authors.lastname: Kvaratskhelia
authors.affiliation: Tbilisi State Medical University, Department of Molecular and Medical Genetics, Georgia.
authors.forename: E
authors.initials: E
authors.lastname: Abzianidze
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001943:Breast Neoplasms / Q000097:blood / Q000201:enzymology* / Q000473:pathology; D018270:Carcinoma, Ductal, Breast / Q000097:blood / Q000201:enzymology* / Q000473:pathology; D016022:Case-Control Studies; D000076123:DNA (Cytosine-5-)-Methyltransferase 1; D004248:DNA (Cytosine-5-)-Methyltransferases / Q000097:blood*; D000090205:DNA Methyltransferase 3A; D005260:Female; D011495:Histone-Lysine N-Methyltransferase / Q000097:blood*; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000201:enzymology*; D008875:Middle Aged; D060787:Neoplasm Grading; D010865:Pilot Projects
chemical_list: C000717967:DNMT3A protein, human; D000076123:DNA (Cytosine-5-)-Methyltransferase 1; D004248:DNA (Cytosine-5-)-Methyltransferases; D000090205:DNA Methyltransferase 3A; C000619380:DNMT1 protein, human; D011495:Histone-Lysine N-Methyltransferase