title: Production of poly(malic acid) from sugarcane juice in fermentation by Aureobasidium pullulans: Kinetics and process economics.
abstract: Poly(β-l-malic acid) (PMA) is a biodegradable polymer with many potential biomedical applications. PMA can be readily hydrolyzed to malic acid (MA), which is widely used as an acidulant in foods and pharmaceuticals. PMA production from sucrose and sugarcane juice by Aureobasidium pullulans ZX-10 was studied in shake-flasks and bioreactors, confirming that sugarcane juice can be used as an economical substrate without any pretreatment or nutrients supplementation. A high PMA titer of 116.3g/L and yield of 0.41g/g were achieved in fed-batch fermentation. A high productivity of 0.66g/L·h was achieved in repeated-batch fermentation with cell recycle. These results compared favorably with those obtained from glucose and other biomass feedstocks. A process economic analysis showed that PMA could be produced from sugarcane juice at a cost of $1.33/kg, offering a cost-competitive bio-based PMA for industrial applications.
authors.affiliation: School of Biological and Chemical Engineering, Zhejiang University of Science & Technology, Hangzhou, Zhejiang 310023, China; William G. Lowrie Department of Chemical and Biomolecular Engineering, The Ohio State University, 151 West Woodruff Avenue, Columbus, OH 43210, USA.
authors.forename: Peilian
authors.initials: P
authors.lastname: Wei
authors.affiliation: William G. Lowrie Department of Chemical and Biomolecular Engineering, The Ohio State University, 151 West Woodruff Avenue, Columbus, OH 43210, USA.
authors.forename: Chi
authors.initials: C
authors.lastname: Cheng
authors.affiliation: Bioprocessing Innovative Company, 4734 Bridle Path Ct., Dublin, OH 43017, USA.
authors.forename: Meng
authors.initials: M
authors.lastname: Lin
authors.affiliation: Bioprocessing Innovative Company, 4734 Bridle Path Ct., Dublin, OH 43017, USA.
authors.forename: Yipin
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: William G. Lowrie Department of Chemical and Biomolecular Engineering, The Ohio State University, 151 West Woodruff Avenue, Columbus, OH 43210, USA. Electronic address: yang.15@osu.edu.
authors.forename: Shang-Tian
authors.initials: ST
authors.lastname: Yang
mesh_terms: D001203:Ascomycota / Q000378:metabolism*; D061249:Batch Cell Culture Techniques; D018533:Biomass; D019149:Bioreactors; D001709:Biotechnology / Q000191:economics / Q000295:instrumentation / Q000379:methods*; D005285:Fermentation; D005947:Glucose / Q000378:metabolism; D007700:Kinetics; D008293:Malates / Q000191:economics* / Q000378:metabolism*; D011108:Polymers / Q000191:economics* / Q000378:metabolism*; D031786:Saccharum / Q000737:chemistry / Q000378:metabolism*; D013395:Sucrose / Q000378:metabolism
chemical_list: D008293:Malates; D011108:Polymers; C121060:poly(malic acid); D013395:Sucrose; D005947:Glucose
keywords: Aureobasidium pullulans; Biopolymer; Fermentation; Polymalic acid; Sucrose; Sugarcane juice