title: Regulation of human cerebrospinal fluid malate dehydrogenase 1 in sporadic Creutzfeldt-Jakob disease patients.
abstract: The identification of reliable diagnostic biomarkers in differential diagnosis of neurodegenerative diseases is an ongoing topic. A previous two-dimensional proteomic study on cerebrospinal fluid (CSF) revealed an elevated level of an enzyme, mitochondrial malate dehydrogenase 1 (MDH1), in sporadic Creutzfeldt-Jakob disease (sCJD) patients. Here, we could demonstrate the expression of MDH1 in neurons as well as in the neuropil. Its levels are lower in sCJD brains than in control brains. An examination of CSF-MDH1 in sCJD patients by ELISA revealed a significant elevation of CSF-MDH1 levels in sCJD patients (independently from thePRNPcodon 129 MV genotype or the prion protein scrapie (PrPSc) type) in comparison to controls. In combination with total tau (tau), CSF-MDH1 detection exhibited a high diagnostic accuracy for sCJD diagnosis with a sensitivity of 97.5% and a specificity of 95.6%. A correlation study of MDH1 level in CSF with other neurodegenerative marker proteins revealed a significant positive correlation between MDH1 concentration with tau, 14-3-3 and neuron specific enolase level. In conclusion, our study indicated the potential of MDH1 in combination with tau as an additional biomarker in sCJD improving diagnostic accuracy of tau markedly.
authors.affiliation: From the Department of Neurology, University Medical Center Göttingen, Göttingen, Germany.|German Center for Neurodegenerative Diseases (DZNE) - Göttingen Campus, Göttingen, Germany.
authors.forename: Matthias
authors.initials: M
authors.lastname: Schmitz
authors.affiliation: From the Department of Neurology, University Medical Center Göttingen, Göttingen, Germany.|German Center for Neurodegenerative Diseases (DZNE) - Göttingen Campus, Göttingen, Germany.
authors.forename: Franc
authors.initials: F
authors.lastname: Llorens
authors.affiliation: From the Department of Neurology, University Medical Center Göttingen, Göttingen, Germany.
authors.forename: Alexander
authors.initials: A
authors.lastname: Pracht
authors.affiliation: From the Department of Neurology, University Medical Center Göttingen, Göttingen, Germany.
authors.forename: Tobias
authors.initials: T
authors.lastname: Thom
authors.affiliation: From the Department of Neurology, University Medical Center Göttingen, Göttingen, Germany.
authors.forename: Ângela
authors.initials: Â
authors.lastname: Correia
authors.affiliation: From the Department of Neurology, University Medical Center Göttingen, Göttingen, Germany.|German Center for Neurodegenerative Diseases (DZNE) - Göttingen Campus, Göttingen, Germany.
authors.forename: Saima
authors.initials: S
authors.lastname: Zafar
authors.affiliation: Institute of Neuropathology, Bellvitge University Hospital, CIBERNED, Hospitalet de Llobregat, University of Barcelona, Barcelona, Spain.
authors.forename: Isidre
authors.initials: I
authors.lastname: Ferrer
authors.affiliation: From the Department of Neurology, University Medical Center Göttingen, Göttingen, Germany.|German Center for Neurodegenerative Diseases (DZNE) - Göttingen Campus, Göttingen, Germany.
authors.forename: Inga
authors.initials: I
authors.lastname: Zerr
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D015415:Biomarkers / Q000134:cerebrospinal fluid; D001921:Brain / Q000473:pathology; D016022:Case-Control Studies; D007562:Creutzfeldt-Jakob Syndrome / Q000134:cerebrospinal fluid* / Q000175:diagnosis; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D008291:Malate Dehydrogenase / Q000134:cerebrospinal fluid* / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D000072002:Prion Proteins / Q000134:cerebrospinal fluid; D012680:Sensitivity and Specificity; D016875:tau Proteins / Q000378:metabolism
chemical_list: D015415:Biomarkers; D000072002:Prion Proteins; D016875:tau Proteins; D008291:Malate Dehydrogenase
keywords: Creutzfeldt-Jakob disease; PRNP codon 129 genotypes; cerebrospinal fluid; diagnostic marker; mitochondrial malate dehydrogenase 1