title: Mouse Immunization with Purified Needle Proteins from Type III Secretion Systems and the Characterization of the Immune Response to These Proteins.
abstract: Many Gram-negative pathogens utilize a type III secretion (T3S) system to directly deliver effector molecules into host eukaryotic cells to manipulate cellular processes. These surface-exposed syringe-like structures are highly conserved, necessary for pathogenesis, and hence are therapeutic targets against a number of Gram-negative pathogens. Here we describe a protocol for using purified needle proteins to immunize mice, and subsequently, ways to characterize the immune response to immunization.
authors.affiliation: Department of Biomedical Sciences, University of North Dakota, 1301 Columbia Road North, Stop 9037, Grand Forks, ND, 58202, USA.
authors.forename: Travis D
authors.initials: TD
authors.lastname: Alvine
authors.affiliation: Department of Biomedical Sciences, University of North Dakota, 1301 Columbia Road North, Stop 9037, Grand Forks, ND, 58202, USA. david.s.bradley@med.und.edu.
authors.forename: David S
authors.initials: DS
authors.lastname: Bradley
authors.affiliation: Department of Biomedical Sciences, School of Medicine and Health Sciences, University of North Dakota, Grand Forks, ND, USA.
authors.forename: Matthew L
authors.initials: ML
authors.lastname: Nilles
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000276:immunology; D001426:Bacterial Proteins / Q000276:immunology*; D016207:Cytokines / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay; D007114:Immunization; D007074:Immunoglobulin G / Q000276:immunology; D051379:Mice; D011994:Recombinant Proteins / Q000008:administration & dosage / Q000276:immunology*; D000069296:Type III Secretion Systems / Q000276:immunology*
chemical_list: D000907:Antibodies, Bacterial; D001426:Bacterial Proteins; D016207:Cytokines; D007074:Immunoglobulin G; D011994:Recombinant Proteins; D000069296:Type III Secretion Systems
keywords: Antibodies; Cytokines; ELISA; Immunization; Type III secretion