title: Validation of a method for sedatives and β-blockers determination in swine, bovine and equine kidney using liquid chromatography coupled with tandem mass spectrometry.
abstract: A method for the determination of five sedatives and 14 β-blocker residues in swine, bovine and equine kidney was validated. Samples were extracted with acetonitrile and purified using dispersive solid phase extraction (d-SPE) with Celite 545 with subsequent analysis by LC-MS/MS. A simplified protocol was applied to validate the method scope extension to include new matrices. Parameters such as recovery, trueness, linearity (r2), relative standard deviation (RSD), decision limit (CCα) and method capability (CCβ) were measured for the bovine and equine kidney matrices. The method was applied to the analysis of more than 300 real samples and is currently included in the Brazilian National Residue Control Plan.
authors.affiliation: a Escola de Química e Alimentos , Universidade Federal do Rio Grande - FURG, Rua Barão do Caí , 125 Santo Antônio da Patrulha , Brazil.
authors.forename: Lenise Guimarães
authors.initials: LG
authors.lastname: de Oliveira
authors.affiliation: b Laboratório Nacional Agropecuário - LANAGRO/RS , Ministério da Agricultura, Pecuária e Abastecimento , Porto Alegre , Brazil.
authors.forename: Fabiano
authors.initials: F
authors.lastname: Barreto
authors.affiliation: b Laboratório Nacional Agropecuário - LANAGRO/RS , Ministério da Agricultura, Pecuária e Abastecimento , Porto Alegre , Brazil.
authors.forename: Rodrigo
authors.initials: R
authors.lastname: Hoff
authors.affiliation: b Laboratório Nacional Agropecuário - LANAGRO/RS , Ministério da Agricultura, Pecuária e Abastecimento , Porto Alegre , Brazil.
authors.forename: Gabriel
authors.initials: G
authors.lastname: Rübensam
authors.affiliation: a Escola de Química e Alimentos , Universidade Federal do Rio Grande - FURG, Rua Barão do Caí , 125 Santo Antônio da Patrulha , Brazil.
authors.forename: Marcia Helena
authors.initials: MH
authors.lastname: Scherer Kurz
authors.affiliation: a Escola de Química e Alimentos , Universidade Federal do Rio Grande - FURG, Rua Barão do Caí , 125 Santo Antônio da Patrulha , Brazil.
authors.forename: Gabriela
authors.initials: G
authors.lastname: Galle
authors.affiliation: a Escola de Química e Alimentos , Universidade Federal do Rio Grande - FURG, Rua Barão do Caí , 125 Santo Antônio da Patrulha , Brazil.
authors.forename: Fábio Ferreira
authors.initials: FF
authors.lastname: Gonçalves
mesh_terms: D000319:Adrenergic beta-Antagonists / Q000378:metabolism*; D000818:Animals; D002417:Cattle; D002853:Chromatography, Liquid / Q000379:methods*; D006736:Horses; D006993:Hypnotics and Sedatives / Q000378:metabolism*; D007668:Kidney / Q000378:metabolism*; D013552:Swine; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D000319:Adrenergic beta-Antagonists; D006993:Hypnotics and Sedatives
keywords: LC–MS/MS; animal tissues; dispersive solid phase extraction; sedatives; β-blockers