title: Investigation on interaction of DNA and several cationic surfactants with different head groups by spectroscopy, gel electrophoresis and viscosity technologies.
abstract: In this study, the interaction between DNA and several cationic surfactants with different head groups such as ethyl hexadecyl dimethyl ammonium bromide (EHDAB), hexadecyl dimethyl benzyl ammonium chloride (HDBAC), and cetyl pyridinium bromide (CPB) were investigated by UV-vis absorption, fluorescence and circular dichroism (CD) spectroscopy, gel electrophoresis, and viscosity technologies. The results show that these cationic surfactants can interact with DNA and major binding modes are electrostatic and hydrophobic. Also, CPB and HDBAC molecules interact with DNA by partial intercalation, and CPB has slightly stronger intercalation than HDBAC, while EHDAB interacts with DNA by non-intercalation. The different head groups of the surfactant molecules can influence the interaction strength. CPB has the stronger interaction with DNA than the others. Moreover, surfactant concentration, the ratio of DNA and fluorescence probe, ionic strength can influence the interaction. The surfactants may interact with DNA by the competition reactions with BR for DNA-BR. The increase of ionic strength may favor the surface binding between DNA and surfactants to some extent. This work provides deep mechanistic insight on the toxicity of cationic surfactants with different head groups to DNA molecules.
authors.affiliation: School of Environment, Liaoning University, Shenyang 110036, PR China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Guo
authors.affiliation: School of Environment, Liaoning University, Shenyang 110036, PR China. Electronic address: lnuhjhx@163.com.
authors.forename: Zhaohong
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: School of Environment, Liaoning University, Shenyang 110036, PR China.
authors.forename: Youtao
authors.initials: Y
authors.lastname: Song
authors.affiliation: Shenyang Red Cross Hospital, Shenyang 110014, PR China.
authors.forename: Shuo
authors.initials: S
authors.lastname: Liu
authors.affiliation: School of Environment, Liaoning University, Shenyang 110036, PR China.
authors.forename: Wei
authors.initials: W
authors.lastname: Gao
authors.affiliation: School of Environment, Liaoning University, Shenyang 110036, PR China; Pony Testing International Group, Qingdao 266100, PR China.
authors.forename: Heng
authors.initials: H
authors.lastname: Qiao
authors.affiliation: School of Environment, Liaoning University, Shenyang 110036, PR China.
authors.forename: Lili
authors.initials: L
authors.lastname: Guo
authors.affiliation: School of Chemistry, Liaoning University, Shenyang 110036, PR China. Electronic address: wangjun888tg@126.com.
authors.forename: Jun
authors.initials: J
authors.lastname: Wang
mesh_terms: D001965:Bromides / Q000737:chemistry; D002942:Circular Dichroism; D004247:DNA / Q000737:chemistry*; D004587:Electrophoresis, Agar Gel; D052244:Endocrine Disruptors / Q000737:chemistry*; D004785:Environmental Pollutants / Q000737:chemistry*; D005456:Fluorescent Dyes; D057927:Hydrophobic and Hydrophilic Interactions; D007364:Intercalating Agents / Q000737:chemistry*; D009994:Osmolar Concentration; D000644:Quaternary Ammonium Compounds / Q000737:chemistry; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet; D055672:Static Electricity; D013501:Surface-Active Agents / Q000737:chemistry*; D014783:Viscosity
chemical_list: D001965:Bromides; D052244:Endocrine Disruptors; D004785:Environmental Pollutants; D005456:Fluorescent Dyes; D007364:Intercalating Agents; D000644:Quaternary Ammonium Compounds; D013501:Surface-Active Agents; D004247:DNA; C427203:calf thymus DNA; C051470:ammonium bromide
keywords: Cationic surfactant; DNA; Gel electrophoresis; Interaction; Spectroscopy; Viscosity