title: Mismatch repair deficiency increases the transfer of antibiosis and antixenosis properties against Colorado potato beetle in somatic hybrids of Solanum tuberosum + S. chacoense.
abstract: BACKGROUND
  Colorado potato beetle (CPB) has become the biggest enemy of cultivated potato worldwide. One of the most effective sources of resistance to CPB is Solanum chacoense, an accession with a high leptine glycoalkaloid content. The aim of our study was to assay the repellence and toxicity of S. chacoense, its somatic hybrids (SHs) and their backcross progenies (BC1) with potato for CPB adults and larvae. Transgenic S. chacoense, deficient in DNA mismatch repair (MMR), was also used to produce SHs, in order to increase homeologous recombination and hence introgression of wild-species DNA into the potato gene pool.
  
  
  RESULTS
  Wild-type SH was highly resistant to CPB. Resistance to CPB of BC1progenies showed a 1:3 inheritance pattern. MMR-deficient SHs performed better in the resistance analysis. Most MMR-deficient SHs had a similar toxicity as S. chacoense and an intensely repellent effect on CPB adults. Resistance of SHs and BC1clones may be attributed to leptine biosynthesis, which was confirmed using a RAPD marker.
  
  
  CONCLUSIONS
  This is the first report of SHs and their progenies exhibiting both antibiosis and antixenosis against CPB. Resistant SHs are an important step forward in combating this voracious pest of potato. © 2016 Society of Chemical Industry.
authors.affiliation: Department of Molecular Biology and Biotechnology, Faculty of Biology and Geology, 'Babes-Bolyai' University, Cluj-Napoca, Romania.
authors.forename: Imola
authors.initials: I
authors.lastname: Molnár
authors.affiliation: Department of Molecular Biology and Biotechnology, Faculty of Biology and Geology, 'Babes-Bolyai' University, Cluj-Napoca, Romania.
authors.forename: Enikő
authors.initials: E
authors.lastname: Besenyei
authors.affiliation: Institute for Breeding Research on Agriculture Crops, Julius Kühn-Institut (JKI), Groß Lüsewitz, Germany.
authors.forename: Ramona
authors.initials: R
authors.lastname: Thieme
authors.affiliation: BTL Bio-Test Lab GmbH, Sagerheide, Germany.
authors.forename: Thomas
authors.initials: T
authors.lastname: Thieme
authors.affiliation: Department of Molecular Biology and Biotechnology, Faculty of Biology and Geology, 'Babes-Bolyai' University, Cluj-Napoca, Romania.
authors.forename: Adriana
authors.initials: A
authors.lastname: Aurori
authors.affiliation: Department of Molecular Biology and Biotechnology, Faculty of Biology and Geology, 'Babes-Bolyai' University, Cluj-Napoca, Romania.
authors.forename: Andreea
authors.initials: A
authors.lastname: Baricz
authors.affiliation: Department of Molecular Biology and Biotechnology, Faculty of Biology and Geology, 'Babes-Bolyai' University, Cluj-Napoca, Romania.
authors.forename: Horia Leonard
authors.initials: HL
authors.lastname: Banciu
authors.affiliation: Department of Molecular Biology and Biotechnology, Faculty of Biology and Geology, 'Babes-Bolyai' University, Cluj-Napoca, Romania.
authors.forename: Elena
authors.identifier: http://orcid.org/0000-0002-0982-8547
authors.initials: E
authors.lastname: Rakosy-Tican
mesh_terms: D000818:Animals; D000898:Antibiosis / Q000235:genetics*; D001517:Coleoptera / Q000187:drug effects*; D053843:DNA Mismatch Repair; D005247:Feeding Behavior / Q000187:drug effects; D007300:Insect Control / Q000379:methods; D007814:Larva / Q000187:drug effects; D030821:Plants, Genetically Modified / Q000235:genetics / Q000378:metabolism; D019105:Random Amplified Polymorphic DNA Technique; D012991:Solanaceous Alkaloids / Q000096:biosynthesis; D011198:Solanum tuberosum / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism
chemical_list: D012991:Solanaceous Alkaloids; C410862:leptine I
keywords: Leptinotarsa decemlineata; MMR deficiency; Solanum chacoense; antibiosis; antixenosis; somatic hybrids