title: Effects of deletion of the ac109 gene of Autographa californica nucleopolyhedrovirus on interactions with mammalian cells.
abstract: Baculoviruses are able to enter into mammalian cells, where they can express a transgene that is placed under an appropriate promoter, without producing infectious progeny. ORF109 encodes an essential baculovirus protein that participates in the interaction of the baculovirus with mammalian cells. To date, the mechanisms underlying this interaction are not yet known. We demonstrated that although a Ac109 knock out virus maintained its ability to enter into BHK-21 cells, there was a marked reduction in the expression efficiency of the nuclear transgene. Moreover, the amount of free cytoplasmic viral DNA, which was detected by transcription of a reporter gene, was severely diminished. These results suggest Ac109 could be involved in maintaining the integrity of the viral nucleic acid.
authors.affiliation: INTA, CONICET, Instituto de Biotecnología, CICVyA, Nicolás Repetto y de los Reseros S/N, Hurlingham, CP 1686, Buenos Aires, Argentina.
authors.forename: Victoria
authors.initials: V
authors.lastname: Alfonso
authors.affiliation: INTA, Instituto de Biotecnología, CICVyA, Nicolás Repetto y de los Reseros S/N, Hurlingham, CP 1686, Buenos Aires, Argentina.
authors.forename: Sabrina
authors.initials: S
authors.lastname: Amalfi
authors.affiliation: INTA, CONICET, Instituto de Biotecnología, CICVyA, Nicolás Repetto y de los Reseros S/N, Hurlingham, CP 1686, Buenos Aires, Argentina.
authors.forename: María Gabriela
authors.initials: MG
authors.lastname: López
authors.affiliation: INTA, CONICET, Instituto de Biotecnología, CICVyA, Nicolás Repetto y de los Reseros S/N, Hurlingham, CP 1686, Buenos Aires, Argentina. taboga.oscaralberto@inta.gob.ar.
authors.forename: Oscar
authors.identifier: http://orcid.org/0000-0001-9575-3045
authors.initials: O
authors.lastname: Taboga
mesh_terms: D000818:Animals; D002460:Cell Line; D006224:Cricetinae; D017353:Gene Deletion; D055786:Gene Knockout Techniques; D017930:Genes, Reporter; D017924:Nucleopolyhedroviruses / Q000235:genetics* / Q000302:isolation & purification / Q000502:physiology; D014764:Viral Proteins / Q000235:genetics* / Q000378:metabolism*; D014776:Virus Cultivation; D014779:Virus Replication
chemical_list: D014764:Viral Proteins
keywords: Baculovirus; ac109; mammalian cells