title: Comparative Transcriptome Analysis of Chinary, Assamica and Cambod tea (Camellia sinensis) Types during Development and Seasonal Variation using RNA-seq Technology.
abstract: Tea quality and yield is influenced by various factors including developmental tissue, seasonal variation and cultivar type. Here, the molecular basis of these factors was investigated in three tea cultivars namely, Him Sphurti (H), TV23 (T), and UPASI-9 (U) using RNA-seq. Seasonal variation in these cultivars was studied during active (A), mid-dormant (MD), dormant (D) and mid-active (MA) stages in two developmental tissues viz. young and old leaf. Development appears to affect gene expression more than the seasonal variation and cultivar types. Further, detailed transcript and metabolite profiling has identified genes such as F3'H, F3'5'H, FLS, DFR, LAR, ANR and ANS of catechin biosynthesis, while MXMT, SAMS, TCS and XDH of caffeine biosynthesis/catabolism as key regulators during development and seasonal variation among three different tea cultivars. In addition, expression analysis of genes related to phytohormones such as ABA, GA, ethylene and auxin has suggested their role in developmental tissues during seasonal variation in tea cultivars. Moreover, differential expression of genes involved in histone and DNA modification further suggests role of epigenetic mechanism in coordinating global gene expression during developmental and seasonal variation in tea. Our findings provide insights into global transcriptional reprogramming associated with development and seasonal variation in tea.
authors.affiliation: Plant Metabolic Engineering Laboratory, Biotechnology Division, CSIR-Institute of Himalayan Bioresource Technology, Palampur-176061 (HP), India.|Academy of Scientific and Innovative Research, New Delhi, India.
authors.forename: Ajay
authors.initials: A
authors.lastname: Kumar
authors.affiliation: Studio of Computational Biology &Bioinformatics, Biotechnology Division, CSIR-Institute of Himalayan Bioresource Technology, Palampur-176061 (HP), India.|Department of Biotechnology, Guru Nanak Dev University, Amritsar, Punjab, India.
authors.forename: Vandna
authors.initials: V
authors.lastname: Chawla
authors.affiliation: Food and Nutraceutical Division, CSIR-Institute of Himalayan Bioresource Technology, Palampur-176061 (HP), India.
authors.forename: Eshita
authors.initials: E
authors.lastname: Sharma
authors.affiliation: Studio of Computational Biology &Bioinformatics, Biotechnology Division, CSIR-Institute of Himalayan Bioresource Technology, Palampur-176061 (HP), India.
authors.forename: Pallavi
authors.initials: P
authors.lastname: Mahajan
authors.affiliation: Academy of Scientific and Innovative Research, New Delhi, India.|Studio of Computational Biology &Bioinformatics, Biotechnology Division, CSIR-Institute of Himalayan Bioresource Technology, Palampur-176061 (HP), India.
authors.forename: Ravi
authors.initials: R
authors.lastname: Shankar
authors.affiliation: Plant Metabolic Engineering Laboratory, Biotechnology Division, CSIR-Institute of Himalayan Bioresource Technology, Palampur-176061 (HP), India.|Academy of Scientific and Innovative Research, New Delhi, India.|Center of Innovative and Applied Bioprocessing (CIAB), Mohali-160071, Punjab, India.
authors.forename: Sudesh Kumar
authors.initials: SK
authors.lastname: Yadav
mesh_terms: D028241:Camellia sinensis / Q000235:genetics / Q000378:metabolism; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant / Q000502:physiology*; D017343:Genes, Plant / Q000502:physiology; D018515:Plant Leaves / Q000235:genetics / Q000378:metabolism; D018749:RNA, Plant / Q000096:biosynthesis / Q000235:genetics; D012621:Seasons; D017423:Sequence Analysis, RNA; D059467:Transcriptome / Q000502:physiology*
chemical_list: D018749:RNA, Plant