title: Dietary 2'-Fucosyllactose Enhances Operant Conditioning and Long-Term Potentiation via Gut-Brain Communication through the Vagus Nerve in Rodents.
abstract: 2´-fucosyllactose (2´-FL) is an abundant human milk oligosaccharide (HMO) in human milk with diverse biological effects. We recently reported ingested 2´-FL stimulates central nervous system (CNS) function, such as hippocampal long term potentiation (LTP) and learning and memory in rats. Conceivably the effect of 2´-FL on CNS function may be via the gut-brain axis (GBA), specifically the vagus nerve, and L-fucose (Fuc) may play a role. This study had two aims: (1) determine if the effect of ingested 2´-FL on the modulation of CNS function is dependent on the integrity of the molecule; and (2) confirm if oral 2´-FL modified hippocampal LTP and associative learning related skills in rats submitted to bilateral subdiaphragmatic vagotomy. Results showed that 2´-FL but not Fuc enhanced LTP, and vagotomy inhibited the effects of oral 2´-FL on LTP and associative learning related paradigms. Taken together, the data show that dietary 2´-FL but not its Fuc moiety affects cognitive domains and improves learning and memory in rats. This effect is dependent on vagus nerve integrity, suggesting GBA plays a role in 2´-FL-mediated cognitive benefits.
authors.affiliation: Strategic R&D Department, Abbott Nutrition, Granada, 18004, Spain.
authors.forename: Enrique
authors.initials: E
authors.lastname: Vazquez
authors.affiliation: Strategic R&D Department, Abbott Nutrition, Granada, 18004, Spain.
authors.forename: Alejandro
authors.initials: A
authors.lastname: Barranco
authors.affiliation: Strategic R&D Department, Abbott Nutrition, Granada, 18004, Spain.
authors.forename: Maria
authors.initials: M
authors.lastname: Ramirez
authors.affiliation: Division of Neurosciences, Pablo de Olavide University, Seville, 41013, Spain.
authors.forename: Agnes
authors.initials: A
authors.lastname: Gruart
authors.affiliation: Division of Neurosciences, Pablo de Olavide University, Seville, 41013, Spain.
authors.forename: Jose M
authors.initials: JM
authors.lastname: Delgado-Garcia
authors.affiliation: Strategic R&D Department, Abbott Nutrition, Granada, 18004, Spain.
authors.forename: Maria L
authors.initials: ML
authors.lastname: Jimenez
authors.affiliation: Strategic R&D Department, Abbott Nutrition, Columbus, OH, United States of America.
authors.forename: Rachael
authors.initials: R
authors.lastname: Buck
authors.affiliation: Strategic R&D Department, Abbott Nutrition, Granada, 18004, Spain.
authors.forename: Ricardo
authors.initials: R
authors.lastname: Rueda
mesh_terms: D000284:Administration, Oral; D000818:Animals; D001921:Brain / Q000187:drug effects / Q000502:physiology*; D003216:Conditioning, Operant / Q000187:drug effects*; D004032:Diet; D004064:Digestive System / Q000187:drug effects*; D005643:Fucose / Q000008:administration & dosage / Q000494:pharmacology; D017774:Long-Term Potentiation / Q000187:drug effects*; D008297:Male; D017207:Rats, Sprague-Dawley; D014312:Trisaccharides / Q000008:administration & dosage / Q000494:pharmacology*; D014628:Vagotomy; D014630:Vagus Nerve / Q000187:drug effects / Q000502:physiology*
chemical_list: D014312:Trisaccharides; D005643:Fucose; C031420:2'-fucosyllactose