title: Energy-dense diets increase FGF23, lead to phosphorus retention and promote vascular calcifications in rats.
abstract: Rats with normal renal function (Experiment 1, n = 12) and uninephrectomized (1/2Nx) rats (Experiment 2, n = 12) were fed diets with normal P (NP) and either normal (NF) or high fat (HF). Rats with intact renal function (Experiment 3, n = 12) were also fed NF or HF diets with high P (HP). Additionally, uremic (5/6Nx) rats (n = 16) were fed HP diets with NF or HF. Feeding the HF diets resulted in significant elevation of plasma FGF23 vs rats fed NF diets: Experiment 1, 593 ± 126 vs 157 ± 28 pg/ml (p < 0.01); Experiment 2, 538 ± 105 vs 250 ± 18 pg/ml (p < 0.05); Experiment 3, 971 ± 118 vs 534 ± 40 pg/ml (p < 0.01). Rats fed HF diets showed P retention and decreased renal klotho (ratio klotho/actin) vs rats fed NF diets: Experiment 1, 0.75 ± 0.06 vs 0.97 ± 0.02 (p < 0.01); Experiment 2, 0.69 ± 0.07 vs 1.12 ± 0.08 (p < 0.01); Experiment 3, 0.57 ± 0.19 vs 1.16 ± 0.15 (p < 0.05). Uremic rats fed HF diet showed more severe vascular calcification (VC) than rats fed NF diet (aortic Ca = 6.3 ± 1.4 vs 1.4 ± 0.1 mg/g tissue, p < 0.001). In conclusion, energy-rich diets increased plasma levels of FGF23, a known risk factor of cardiovascular morbidity and mortality. Even though FGF23 has major phosphaturic actions, feeding HF diets resulted in P retention, likely secondary to decreased renal klotho, and aggravated uremic VC.
authors.affiliation: Dept Medicina y Cirugia Animal, Universidad de Cordoba, Cordoba, Spain.
authors.forename: Ana I
authors.initials: AI
authors.lastname: Raya
authors.affiliation: Dept Medicina y Cirugia Animal, Universidad de Cordoba, Cordoba, Spain.
authors.forename: Rafael
authors.initials: R
authors.lastname: Rios
authors.affiliation: Dept Medicina y Cirugia Animal, Universidad de Cordoba, Cordoba, Spain.
authors.forename: Carmen
authors.initials: C
authors.lastname: Pineda
authors.affiliation: Laboratorio de Nefrologia. IIS-Fundacion Jimenez-Diaz, REDinREN, Madrid, Spain.
authors.forename: Maria E
authors.initials: ME
authors.lastname: Rodriguez-Ortiz
authors.affiliation: Dept Medicina y Cirugia Animal, Universidad de Cordoba, Cordoba, Spain.
authors.forename: Elisa
authors.initials: E
authors.lastname: Diez
authors.affiliation: Unidad de Lipidos y Aterosclerosis. IMIBIC/Hospital Universitario Reina Sofia and CIBER Fisiopatologia Obesidad y Nutricion (CIBEROBN), Instituto de Salud Carlos III, Spain.
authors.forename: Yolanda
authors.initials: Y
authors.lastname: Almaden
authors.affiliation: Servicio de Nefrologia, REDinREN, IMIBIC/Hospital Universitario Reina Sofia, Cordoba, Spain.
authors.forename: Juan R
authors.initials: JR
authors.lastname: Muñoz-Castañeda
authors.affiliation: Servicio de Nefrologia, REDinREN, IMIBIC/Hospital Universitario Reina Sofia, Cordoba, Spain.
authors.forename: Mariano
authors.initials: M
authors.lastname: Rodriguez
authors.affiliation: Dept Medicina y Cirugia Animal, Universidad de Cordoba, Cordoba, Spain.
authors.forename: Escolastico
authors.initials: E
authors.lastname: Aguilera-Tejero
authors.affiliation: Dept Medicina y Cirugia Animal, Universidad de Cordoba, Cordoba, Spain.
authors.forename: Ignacio
authors.initials: I
authors.lastname: Lopez
mesh_terms: D000818:Animals; D001835:Body Weight; D004041:Dietary Fats / Q000009:adverse effects*; D005346:Fibroblast Growth Factors / Q000097:blood*; D005786:Gene Expression Regulation / Q000187:drug effects; D005966:Glucuronidase / Q000378:metabolism; D007668:Kidney / Q000378:metabolism / Q000502:physiology; D000090265:Klotho Proteins; D008297:Male; D010758:Phosphorus / Q000378:metabolism*; D051381:Rats; D014511:Uremia / Q000150:complications* / Q000378:metabolism; D061205:Vascular Calcification / Q000139:chemically induced* / Q000378:metabolism
chemical_list: D004041:Dietary Fats; C505605:Fgf23 protein, rat; D010758:Phosphorus; D005346:Fibroblast Growth Factors; D005966:Glucuronidase; D000090265:Klotho Proteins