title: Highlighting Gibberellins Accumulation Sites in Arabidopsis thaliana Root Using Fluorescently Labeled Gibberellins.
abstract: The physical location of plant hormones is an important factor in maintaining their proper metabolism, perception, and mediated developmental responses. Thus, unveiling plant hormones dynamics at the molecule's level is essential for a comprehensive, detailed understanding of both their functions and the regulative mechanisms they are subjected to. Here, we describe the use of fluorescently labeled, bioactive gibberellins (GAs) to highlight the dynamic distribution and accumulation sites of bioactive GAs in Arabidopsis thaliana roots by confocal microscopy.
authors.affiliation: Department of Molecular Biology and Ecology of Plants, Life Sciences Faculty, Tel-Aviv University, PO Box 39040, 6997801, Tel-Aviv, Israel.
authors.forename: Hilla
authors.initials: H
authors.lastname: Schayek
authors.affiliation: Department of Molecular Biology and Ecology of Plants, Life Sciences Faculty, Tel-Aviv University, PO Box 39040, 6997801, Tel-Aviv, Israel.
authors.forename: Eilon
authors.initials: E
authors.lastname: Shani
authors.affiliation: Department of Molecular Biology and Ecology of Plants, Life Sciences Faculty, Tel-Aviv University, PO Box 39040, 6997801, Tel-Aviv, Israel. royweinstain@post.tau.ac.il.
authors.forename: Roy
authors.initials: R
authors.lastname: Weinstain
mesh_terms: D017360:Arabidopsis / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000378:metabolism; D018506:Gene Expression Regulation, Plant / Q000502:physiology; D005875:Gibberellins / Q000378:metabolism*; D010937:Plant Growth Regulators / Q000378:metabolism; D018517:Plant Roots / Q000378:metabolism*
chemical_list: D029681:Arabidopsis Proteins; D005875:Gibberellins; D010937:Plant Growth Regulators
keywords: Chemical biology; Fluorescence microscopy; Fluorescent labeling; Gibberellins; Plant hormones