title: Activity control of autodisplayed proteins on the same outer membrane layer of E. coli by using Z-domain/streptavidin/and lipase/foldase systems.
abstract: The autodisplay technology has been applied for expression of a desired protein on the outer membrane (OM) of Escherichia coli. In this work, the OM fractions of E. coli with two autodisplayed proteins were separately prepared and mixed to demonstrate the feasibility of control over the ratio of two autodisplayed proteins. As the first model, Z-domain and streptavidin were autodisplayed, and their activities were tested by means of the combined OM layer in a 96-well microplate and a surface plasmon resonance (SPR) biosensor. As the second model, lipase and foldase were autodisplayed which required an interaction between two proteins to obtain the activity of lipase. The OM fractions of E. coli with an autodisplayed lipase and foldase were separately prepared and mixed to demonstrate the feasibility of control over the ratio of two autodisplayed proteins when the interaction of two proteins is required within the same OM layer for the activity of the lipase.
authors.affiliation: Department of Materials Science and Engineering, Yonsei University, 50 Yonsei-ro, Seo-dae-mun-gu, Seoul 120-749, Republic of Korea.
authors.forename: Seo-Yoon
authors.initials: SY
authors.lastname: Chang
authors.affiliation: Department of Materials Science and Engineering, Yonsei University, 50 Yonsei-ro, Seo-dae-mun-gu, Seoul 120-749, Republic of Korea.
authors.forename: Ji-Hong
authors.initials: JH
authors.lastname: Bong
authors.affiliation: Department of Materials Science and Engineering, Yonsei University, 50 Yonsei-ro, Seo-dae-mun-gu, Seoul 120-749, Republic of Korea.
authors.forename: Gu
authors.initials: G
authors.lastname: Yoo
authors.affiliation: College of Life Science and Bioengineering, Incheon National University, Republic of Korea.
authors.forename: Misu
authors.initials: M
authors.lastname: Lee
authors.affiliation: Korea Institute of Science and Technology (KIST), Seoul, Republic of Korea.
authors.forename: Min-Jung
authors.initials: MJ
authors.lastname: Kang
authors.affiliation: Institute of Pharmaceutical and Medical Chemistry, University of Muenster, Muenster, Germany.
authors.forename: Joachim
authors.initials: J
authors.lastname: Jose
authors.affiliation: Department of Materials Science and Engineering, Yonsei University, 50 Yonsei-ro, Seo-dae-mun-gu, Seoul 120-749, Republic of Korea. Electronic address: jcpyun@yonsei.ac.kr.
authors.forename: Jae-Chul
authors.initials: JC
authors.lastname: Pyun
mesh_terms: D002462:Cell Membrane / Q000378:metabolism; D061505:Cell Surface Display Techniques / Q000379:methods*; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism*; D008049:Lipase / Q000235:genetics / Q000378:metabolism; D000072417:Protein Domains; D011993:Recombinant Fusion Proteins / Q000235:genetics* / Q000378:metabolism*; D019809:Streptavidin / Q000235:genetics / Q000378:metabolism
chemical_list: D011993:Recombinant Fusion Proteins; D019809:Streptavidin; D008049:Lipase; C504169:lipase foldase
keywords: Autodisplay; Co-transformation; Foldase; Lipase; Streptavidin; Z-domain