title: Prenatal deletion ofDNA methyltransferase 1in neural stem cells impairs neurogenesis and causes anxiety-like behavior in adulthood.
abstract: Despite recent advances in our understanding of epigenetic regulation of central nervous system development, little is known regarding the effects of epigenetic dysregulation on neurogenesis and brain function in adulthood. In the present study, we show that prenatal deletion ofDNA methyltransferase 1 (Dnmt1)in neural stem cells results in impaired neurogenesis as well as increases in inflammatory features (e.g., elevated glial fibrillary acidic protein [GFAP] expression in astrocytes and increased numbers of microglia) in the adult mouse brain. Moreover, these mice exhibited anxiety-like behavior during an open-field test. These findings suggest thatDnmt1plays a critical role in regulating neurogenesis and behavior in the developing brain and into adulthood.
authors.affiliation: Stem Cell Biology and Medicine, Department of Stem Cell Biology and Medicine, Graduate School of Medical Sciences, Kyushu University , Fukuoka, Japan.
authors.forename: Hirofumi
authors.initials: H
authors.lastname: Noguchi
authors.affiliation: Stem Cell Biology and Medicine, Department of Stem Cell Biology and Medicine, Graduate School of Medical Sciences, Kyushu University , Fukuoka, Japan.
authors.forename: Ayaka
authors.initials: A
authors.lastname: Kimura
authors.affiliation: Stem Cell Biology and Medicine, Department of Stem Cell Biology and Medicine, Graduate School of Medical Sciences, Kyushu University , Fukuoka, Japan.
authors.forename: Naoya
authors.initials: N
authors.lastname: Murao
authors.affiliation: Molecular Neurophysiology Research Group, Biomedical Research Institute, National Institute of Advanced Industrial Science and Technology , Ibaraki, Japan.
authors.forename: Masakazu
authors.initials: M
authors.lastname: Namihira
authors.affiliation: Stem Cell Biology and Medicine, Department of Stem Cell Biology and Medicine, Graduate School of Medical Sciences, Kyushu University , Fukuoka, Japan.
authors.forename: Kinichi
authors.initials: K
authors.lastname: Nakashima
keywords: Dnmt1; anxiety; dentate gyrus; hippocampus; neurogenesis