title: Altered uterine contractility in response to β-adrenoceptor agonists in ovarian cancer.
abstract: We aimed to prospectively examine β-adrenoceptor-mediated uterine contractility in women suffering from gynecological malignancies. Myometrial specimens were obtained from non-pregnant women undergoing hysterectomy for benign gynecological disorders, and ovarian, endometrial, synchronous ovarian-endometrial, and cervical cancer. Contractions of myometrial strips in an organ bath before and after cumulative dosages of β2- and β3-adrenoceptor agonists with preincubation of propranolol, SR 59230A, and butoxamine were studied. All agonists induced a dose-dependent attenuation for uterine contractility in endometrial or cervical cancer, similar to that observed in the reference group. Contradictory effects were observed for ovarian cancer alone or in combination with endometrial cancer. CL 316243 or ritodrine abolished the relaxation, whereas BRL 37344 increased the uterine contractility in ovarian cancer. Moreover, β-adrenoceptor antagonists caused varied effects for β2- or β3-adrenoceptor agonists. Our experiments demonstrate that ovarian cancer, alone or as synchronous ovarian-endometrial cancer, substantially alters uterine contractility in response to β-adrenoceptor agonists.
authors.affiliation: Department of Biophysics, Medical University of Białystok, Mickiewicza 2A, 15-089, Białystok, Poland. beata.modzelewska@umb.edu.pl.
authors.forename: Beata
authors.identifier: http://orcid.org/0000-0002-1051-7906
authors.initials: B
authors.lastname: Modzelewska
authors.affiliation: Department of Gynecology and Gynecologic Oncology, Medical University of Białystok, Białystok, Poland.
authors.forename: Maciej
authors.initials: M
authors.lastname: Jóźwik
authors.affiliation: Department of Gynecology, Gynecologic Endocrinology and Obstetrics, Faculty of Medical Sciences, University of Warmia and Mazury, Olsztyn, Poland.
authors.forename: Marcin
authors.initials: M
authors.lastname: Jóźwik
authors.affiliation: Department of General Pathomorphology, Medical University of Białystok, Białystok, Poland.
authors.forename: Stanisław
authors.initials: S
authors.lastname: Sulkowski
authors.affiliation: Department of Biophysics, Medical University of Białystok, Mickiewicza 2A, 15-089, Białystok, Poland.|Department of Experimental Physiology and Pathophysiology, Medical University of Białystok, Białystok, Poland.
authors.forename: Anna
authors.initials: A
authors.lastname: Pędzińska-Betiuk
authors.affiliation: Department of Biophysics, Medical University of Białystok, Mickiewicza 2A, 15-089, Białystok, Poland.
authors.forename: Tomasz
authors.initials: T
authors.lastname: Kleszczewski
authors.affiliation: Department of Biophysics, Medical University of Białystok, Mickiewicza 2A, 15-089, Białystok, Poland.|Łomza Medical College of the Universal Educational Society, Łomża, Poland.
authors.forename: Anna
authors.initials: A
authors.lastname: Kostrzewska
mesh_terms: D058667:Adrenergic beta-3 Receptor Agonists / Q000494:pharmacology; D000318:Adrenergic beta-Agonists / Q000494:pharmacology; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D004149:Dioxoles / Q000494:pharmacology*; D016889:Endometrial Neoplasms; D004983:Ethanolamines / Q000494:pharmacology*; D005260:Female; D006801:Humans; D008875:Middle Aged; D010051:Ovarian Neoplasms; D011943:Receptors, Adrenergic, beta / Q000502:physiology; D012312:Ritodrine / Q000494:pharmacology*; D014590:Uterine Contraction / Q000187:drug effects / Q000502:physiology*
chemical_list: D058667:Adrenergic beta-3 Receptor Agonists; D000318:Adrenergic beta-Agonists; D004149:Dioxoles; D004983:Ethanolamines; D011943:Receptors, Adrenergic, beta; C076126:disodium (R,R)-5-(2-((2-(3-chlorophenyl)-2-hydroxyethyl)-amino)propyl)-1,3-benzodioxole-2,3-dicarboxylate; C057368:BRL 37344; D012312:Ritodrine
keywords: Cervical cancer; Endometrial cancer; Ovarian cancer; Uterine contraction; β2-Adrenoceptors; β3-Adrenoceptors