title: Bioactive spirans and other constituents from the leaves of Cannabis sativa f. sativa.
abstract: In this paper, 17 compounds (1-17) were isolated from the leaves of Hemp (Cannabis sativa f. sativa). Among the isolates, two were determined to be new spirans: cannabispirketal (1), and α-cannabispiranol 4'-O-β-D-glucopyranose (2) by 1D and 2D NMR spectroscopy, LC-MS, and HRESIMS. The known compounds 7, 8, 10, 13, 15, and 16 were isolated from Hemp (C. sativa f. sativa) for the first time. Furthermore, compounds 8 and 13 were isolated from the nature for the first time. All isolated compounds were evaluated for cytotoxicity on different tissue-derived passage cancer cell lines through cell viability and apoptosis assay. Among these compounds, compounds 5, 9 and 16 exhibited a broad-spectrum antitumor effect via inhibiting cell proliferation and promoting apoptosis. These results obtained have provided valuable clues to the understanding of the cytotoxic profile for these isolated compounds from Hemp (C. sativa f. sativa).
authors.affiliation: a Department of Pharmaceutical Engineering, College of Chemical Engineering , Northwest University , Xi'an 710069 , China.
authors.forename: Tian-Tian
authors.initials: TT
authors.lastname: Guo
authors.affiliation: b Quartermaster Research Institute of the General Logistic Department , China-Hemp Research Center , Beijing 100082 , China.
authors.forename: Jian-Chun
authors.initials: JC
authors.lastname: Zhang
authors.affiliation: c Laboratory Animal Center , Fourth Military Medical University , Xi'an 710032 , China.
authors.forename: Hai
authors.initials: H
authors.lastname: Zhang
authors.affiliation: a Department of Pharmaceutical Engineering, College of Chemical Engineering , Northwest University , Xi'an 710069 , China.
authors.forename: Qing-Chao
authors.initials: QC
authors.lastname: Liu
authors.affiliation: c Laboratory Animal Center , Fourth Military Medical University , Xi'an 710032 , China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: a Department of Pharmaceutical Engineering, College of Chemical Engineering , Northwest University , Xi'an 710069 , China.
authors.forename: Yu-Fei
authors.initials: YF
authors.lastname: Hou
authors.affiliation: a Department of Pharmaceutical Engineering, College of Chemical Engineering , Northwest University , Xi'an 710069 , China.
authors.forename: Lu
authors.initials: L
authors.lastname: Bai
authors.affiliation: a Department of Pharmaceutical Engineering, College of Chemical Engineering , Northwest University , Xi'an 710069 , China.
authors.forename: Li
authors.initials: L
authors.lastname: Zhang
authors.affiliation: b Quartermaster Research Institute of the General Logistic Department , China-Hemp Research Center , Beijing 100082 , China.
authors.forename: Xue-Qiang
authors.initials: XQ
authors.lastname: Liu
authors.affiliation: d Department of Medicinal Chemistry, School of Pharmacy , Fourth Military Medical University , Xi'an 710032 , China.
authors.forename: Xue-Ying
authors.initials: XY
authors.lastname: Liu
authors.affiliation: d Department of Medicinal Chemistry, School of Pharmacy , Fourth Military Medical University , Xi'an 710032 , China.
authors.forename: Sheng-Yong
authors.initials: SY
authors.lastname: Zhang
authors.affiliation: a Department of Pharmaceutical Engineering, College of Chemical Engineering , Northwest University , Xi'an 710069 , China.
authors.forename: Nai-Sheng
authors.initials: NS
authors.lastname: Bai
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D047310:Apigenin; D002188:Cannabis / Q000737:chemistry*; D004354:Drug Screening Assays, Antitumor; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D047309:Flavones; D005419:Flavonoids / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D006801:Humans; D061986:MCF-7 Cells; D015394:Molecular Structure; D018515:Plant Leaves / Q000737:chemistry*; D013141:Spiro Compounds / Q000737:chemistry / Q000302:isolation & purification*
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D004365:Drugs, Chinese Herbal; D047309:Flavones; D005419:Flavonoids; D013141:Spiro Compounds; D047310:Apigenin; C045457:cannflavin; C032731:vitexin
keywords: Cannabaceae; Cannabis sativa f. sativa; cytotoxicity; flavonoids; spirans