title: Sesamin inhibits IL-1β-stimulated inflammatory response in human osteoarthritis chondrocytes by activating Nrf2 signaling pathway.
abstract: Sesamin, a bioactive component extracted from sesame, has been reported to exert anti-inflammatory and anti-oxidant effects. In this study, we evaluated the anti-inflammatory effects of sesamin on IL-1β-stimulated human osteoarthritis chondrocytes and investigated the possible mechanism. Results demonstrated that sesamin treatment significantly inhibited PGE2 and NO production induced by IL-1β. Sesamin inhibited MMP1, MMP3, and MMP13 production in IL-1β-stimulated chondrocytes. Sesamin also inhibited IL-1β-induced phosphorylation of NF-κB p65 and IκBα. Meanwhile, sesamin was found to up-regulate the expression of Nrf2 and HO-1. However, Nrf2 siRNA reversed the anti-inflammatory effects of sesamin. In conclusion, our results suggested that sesamin showed anti-inflammatory effects in IL-1β-stimulated chondrocytes by activating Nrf2 signaling pathway.
authors.affiliation: Department of Orthopedics Surgery, The Second Affiliated Hospital of Harbin Medical University, Harbin 150001, China.
authors.forename: Pengyu
authors.initials: P
authors.lastname: Kong
authors.affiliation: Department of Orthopedics Surgery, The Second Affiliated Hospital of Harbin Medical University, Harbin 150001, China.
authors.forename: Guanghua
authors.initials: G
authors.lastname: Chen
authors.affiliation: Department of Orthopedics Surgery, The Second Affiliated Hospital of Harbin Medical University, Harbin 150001, China.
authors.forename: Anlong
authors.initials: A
authors.lastname: Jiang
authors.affiliation: Department of Orthopedics Surgery, The Second Affiliated Hospital of Harbin Medical University, Harbin 150001, China.
authors.forename: Yufu
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Orthopedics Surgery, The Second Affiliated Hospital of Harbin Medical University, Harbin 150001, China.
authors.forename: Chengchao
authors.initials: C
authors.lastname: Song
authors.affiliation: Department of Orthopedics Surgery, The Second Affiliated Hospital of Harbin Medical University, Harbin 150001, China.
authors.forename: Jinpeng
authors.initials: J
authors.lastname: Zhuang
authors.affiliation: Department of Orthopedics Surgery, The Second Affiliated Hospital of Harbin Medical University, Harbin 150001, China.
authors.forename: Chunyang
authors.initials: C
authors.lastname: Xi
authors.affiliation: Department of Orthopedics Surgery, The Second Affiliated Hospital of Harbin Medical University, Harbin 150001, China.
authors.forename: Guangxi
authors.initials: G
authors.lastname: Wang
authors.affiliation: Department of Orthopedics Surgery, The Second Affiliated Hospital of Harbin Medical University, Harbin 150001, China.
authors.forename: Ye
authors.initials: Y
authors.lastname: Ji
authors.affiliation: Department of Orthopedics Surgery, The Second Affiliated Hospital of Harbin Medical University, Harbin 150001, China.
authors.forename: Jinglong
authors.initials: J
authors.lastname: Yan
mesh_terms: D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D019902:Chondrocytes / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism; D015232:Dinoprostone / Q000378:metabolism; D004149:Dioxoles / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D051547:Heme Oxygenase-1 / Q000378:metabolism; D006801:Humans; D053583:Interleukin-1beta / Q000494:pharmacology*; D017705:Lignans / Q000494:pharmacology*; D053505:Matrix Metalloproteinases, Secreted / Q000378:metabolism; D008875:Middle Aged; D051267:NF-E2-Related Factor 2 / Q000235:genetics / Q000378:metabolism*; D000072000:NF-KappaB Inhibitor alpha / Q000378:metabolism; D009569:Nitric Oxide / Q000378:metabolism; D020370:Osteoarthritis, Knee / Q000188:drug therapy* / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D010766:Phosphorylation; D034622:RNA Interference; D015398:Signal Transduction / Q000187:drug effects*; D051996:Transcription Factor RelA / Q000378:metabolism; D014162:Transfection
chemical_list: D000893:Anti-Inflammatory Agents; D004149:Dioxoles; C508585:IL1B protein, human; D053583:Interleukin-1beta; D017705:Lignans; D051267:NF-E2-Related Factor 2; C495635:NFE2L2 protein, human; C000606204:NFKBIA protein, human; C498143:RELA protein, human; D051996:Transcription Factor RelA; D000072000:NF-KappaB Inhibitor alpha; D009569:Nitric Oxide; C496541:HMOX1 protein, human; D051547:Heme Oxygenase-1; D053505:Matrix Metalloproteinases, Secreted; D015232:Dinoprostone; C054125:sesamin
keywords: IL-1β; NF-κB; Nrf2; osteoarthritis chondrocyte; sesamin