title: Nanoencapsulation of coenzyme Q10 and vitamin E acetate protects against UVB radiation-induced skin injury in mice.
abstract: This study aimed to investigate the feasibility of producing semisolid formulations based on nanocapsule suspensions containing the association of the coenzyme Q10 and vitamin E acetate by adding gellan gum (2%) to the suspensions. Furthermore, we studied their application as an alternative for the treatment of inflammation induced by ultraviolet B (UVB) radiation. For this, an animal model of injury induced by UVB-radiation was employed. All semisolids presented pH close to 5.5, drug content above 95% and mean diameter on the nanometric range, after redispersion in water. Besides, the semisolids presented non-Newtonian flow with pseudoplastic behavior and suitable spreadability factor values. The results also showed that the semisolid containing coenzyme Q10-loaded nanocapsules with higher vitamin E acetate concentration reduced in 73±8% the UVB radiation-induced ear edema. Moreover, all formulations tested were able to reduce inflammation parameters evaluated through MPO activity and histological procedure on injured tissue and the semisolids containing the nanoencapsulated coenzyme Q10 reduced oxidative parameters assessment through the non-protein thiols levels and lipid peroxidation. This way, the semisolids based on nanocapsules may be considered a promising approach for the treatment and prevention of skin inflammation diseases.
authors.affiliation: Programa de Pós-Graduação em Ciências Farmacêuticas, Centro de Ciências da Saúde, Universidade Federal de Santa Maria, Santa Maria 97105-900, Brazil.
authors.forename: Natháli S
authors.initials: NS
authors.lastname: Pegoraro
authors.affiliation: Departamento de Farmácia Industrial, Universidade Federal de Santa Maria, Santa Maria 97105-900, Brazil.
authors.forename: Allanna V
authors.initials: AV
authors.lastname: Barbieri
authors.affiliation: Programa de Pós-Graduação em Ciências Biológicas, Bioquímica Toxicológica, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria, Santa Maria 97105-900, Brazil.
authors.forename: Camila
authors.initials: C
authors.lastname: Camponogara
authors.affiliation: Programa de Pós-Graduação em Ciências Farmacêuticas, Centro de Ciências da Saúde, Universidade Federal de Santa Maria, Santa Maria 97105-900, Brazil.
authors.forename: Juliane
authors.initials: J
authors.lastname: Mattiazzi
authors.affiliation: Programa de Pós-Graduação em Ciências Biológicas, Bioquímica Toxicológica, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria, Santa Maria 97105-900, Brazil.
authors.forename: Evelyne S
authors.initials: ES
authors.lastname: Brum
authors.affiliation: Programa de Pós-Graduação em Ciências Farmacêuticas, Centro de Ciências da Saúde, Universidade Federal de Santa Maria, Santa Maria 97105-900, Brazil.
authors.forename: Marila C L
authors.initials: MCL
authors.lastname: Marchiori
authors.affiliation: Programa de Pós-Graduação em Ciências Biológicas, Bioquímica Toxicológica, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria, Santa Maria 97105-900, Brazil.
authors.forename: Sara M
authors.initials: SM
authors.lastname: Oliveira
authors.affiliation: Programa de Pós-Graduação em Ciências Farmacêuticas, Centro de Ciências da Saúde, Universidade Federal de Santa Maria, Santa Maria 97105-900, Brazil; Departamento de Farmácia Industrial, Universidade Federal de Santa Maria, Santa Maria 97105-900, Brazil. Electronic address: leticiacruz@smail.ufsm.br.
authors.forename: Letícia
authors.initials: L
authors.lastname: Cruz
mesh_terms: D000118:Acetylglucosaminidase / Q000378:metabolism; D000279:Administration, Cutaneous; D000818:Animals; D004487:Edema / Q000188:drug therapy / Q000473:pathology; D006863:Hydrogen-Ion Concentration; D007249:Inflammation; D007962:Leukocytes / Q000166:cytology; D008027:Light; D015227:Lipid Peroxidation; D051379:Mice; D053769:Nanocapsules / Q000737:chemistry; D049329:Nanostructures / Q000737:chemistry*; D018384:Oxidative Stress; D010316:Particle Size; D009195:Peroxidase / Q000737:chemistry; D011134:Polysaccharides / Q000737:chemistry; D011135:Polysaccharides, Bacterial / Q000737:chemistry; D011832:Radiation Injuries / Q000188:drug therapy* / Q000503:physiopathology; D012212:Rheology; D012867:Skin / Q000187:drug effects / Q000528:radiation effects*; D013438:Sulfhydryl Compounds / Q000737:chemistry; D013471:Sunburn / Q000517:prevention & control; D024505:Tocopherols / Q000737:chemistry*; D014451:Ubiquinone / Q000031:analogs & derivatives* / Q000737:chemistry; D014466:Ultraviolet Rays
chemical_list: D053769:Nanocapsules; D011134:Polysaccharides; D011135:Polysaccharides, Bacterial; D013438:Sulfhydryl Compounds; D014451:Ubiquinone; C048288:gellan gum; D009195:Peroxidase; D000118:Acetylglucosaminidase; C024989:coenzyme Q10; D024505:Tocopherols
keywords: Coenzyme Q10; Ear edema; Nanocapsules; Skin; UV radiation; Vitamin E