title: A silk peptide fraction restores cognitive function in AF64A-induced Alzheimer disease model rats by increasing expression of choline acetyltransferase gene.
abstract: This study investigated the effects of a silk peptide fraction obtained by incubating silk proteins with Protease N and Neutrase (SP-NN) on cognitive dysfunction of Alzheimer disease model rats. In order to elucidate underlying mechanisms, the effect of SP-NN on the expression of choline acetyltransferase (ChAT) mRNA was assessed in F3.ChAT neural stem cells and Neuro2a neuroblastoma cells; active amino acid sequence was identified using HPLC-MS. The expression of ChAT mRNA in F3.ChAT cells increased by 3.79-fold of the control level by treatment with SP-NN fraction. The active peptide in SP-NN was identified as tyrosine-glycine with 238.1 of molecular weight. Male rats were orally administered with SP-NN (50 or 300mg/kg) and challenged with a cholinotoxin AF64A. As a result of brain injury and decreased brain acetylcholine level, AF64A induced astrocytic activation, resulting in impairment of learning and memory function. Treatment with SP-NN exerted recovering activities on acetylcholine depletion and brain injury, as well as cognitive deficit induced by AF64A. The results indicate that, in addition to a neuroprotective activity, the SP-NN preparation restores cognitive function of Alzheimer disease model rats by increasing the release of acetylcholine.
authors.affiliation: College of Veterinary Medicine, Veterinary Medical Center, Chungbuk National University, Cheongju, Republic of Korea.
authors.forename: Yeseul
authors.initials: Y
authors.lastname: Cha
authors.affiliation: Department of Food Science and Technology, Chungbuk National University, Cheongju, Republic of Korea.
authors.forename: Sang Hoon
authors.initials: SH
authors.lastname: Lee
authors.affiliation: Division of Marine Molecular Biotechnology, Gangneung-Wonju National University, Gangneung, Republic of Korea.
authors.forename: Su Kil
authors.initials: SK
authors.lastname: Jang
authors.affiliation: College of Veterinary Medicine, Veterinary Medical Center, Chungbuk National University, Cheongju, Republic of Korea.
authors.forename: Haiyu
authors.initials: H
authors.lastname: Guo
authors.affiliation: College of Veterinary Medicine, Veterinary Medical Center, Chungbuk National University, Cheongju, Republic of Korea.
authors.forename: Young-Hwan
authors.initials: YH
authors.lastname: Ban
authors.affiliation: College of Veterinary Medicine, Veterinary Medical Center, Chungbuk National University, Cheongju, Republic of Korea.
authors.forename: Dongsun
authors.initials: D
authors.lastname: Park
authors.affiliation: Department of Food Science and Technology, Chungbuk National University, Cheongju, Republic of Korea.
authors.forename: Gwi Yeong
authors.initials: GY
authors.lastname: Jang
authors.affiliation: Department of Food Science and Technology, Chungbuk National University, Cheongju, Republic of Korea.
authors.forename: Sungho
authors.initials: S
authors.lastname: Yeon
authors.affiliation: Worldway Co., Ltd., Sejong, Republic of Korea.
authors.forename: Jeong-Yong
authors.initials: JY
authors.lastname: Lee
authors.affiliation: College of Veterinary Medicine, Veterinary Medical Center, Chungbuk National University, Cheongju, Republic of Korea.
authors.forename: Ehn-Kyoung
authors.initials: EK
authors.lastname: Choi
authors.affiliation: Division of Marine Molecular Biotechnology, Gangneung-Wonju National University, Gangneung, Republic of Korea.
authors.forename: Seong Soo
authors.initials: SS
authors.lastname: Joo
authors.affiliation: Department of Food Science and Technology, Chungbuk National University, Cheongju, Republic of Korea. Electronic address: hsjeong@cbu.ac.kr.
authors.forename: Heon-Sang
authors.initials: HS
authors.lastname: Jeong
authors.affiliation: College of Veterinary Medicine, Veterinary Medical Center, Chungbuk National University, Cheongju, Republic of Korea. Electronic address: solar93@cbu.ac.kr.
authors.forename: Yun-Bae
authors.initials: YB
authors.lastname: Kim
mesh_terms: D000544:Alzheimer Disease / Q000139:chemically induced / Q000523:psychology*; D000818:Animals; D001362:Avoidance Learning / Q000187:drug effects; D001388:Aziridines / Q000633:toxicity*; D045744:Cell Line, Tumor; D002794:Choline / Q000031:analogs & derivatives* / Q000633:toxicity; D002795:Choline O-Acetyltransferase / Q000235:genetics*; D003071:Cognition / Q000187:drug effects*; D004195:Disease Models, Animal; D015971:Gene Expression Regulation, Enzymologic; D019476:Insect Proteins / Q000737:chemistry*; D008297:Male; D018782:Maze Learning / Q000187:drug effects; D010446:Peptide Fragments / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D047011:Silk / Q000737:chemistry*
chemical_list: D001388:Aziridines; D019476:Insect Proteins; D010446:Peptide Fragments; D047011:Silk; C044894:ethylcholine aziridinium; D002795:Choline O-Acetyltransferase; D002794:Choline
keywords: Acetylcholine; Alzheimer disease; Choline acetyltransferase; Cognitive function; Silk peptide