title: Oxidative costs of reproduction in mouse strains selected for different levels of food intake and which differ in reproductive performance.
abstract: Oxidative damage caused by reactive oxygen species has been hypothesised to underpin the trade-off between reproduction and somatic maintenance, i.e., the life-history-oxidative stress theory. Previous tests of this hypothesis have proved equivocal, and it has been suggested that the variation in responses may be related to the tissues measured. Here, we measured oxidative damage (protein carbonyls, 8-OHdG) and antioxidant protection (enzymatic antioxidant activity and serum antioxidant capacity) in multiple tissues of reproductive (R) and non-reproductive (N) mice from two mouse strains selectively bred for high (H) or low (L) food intake, which differ in their reproductive performance, i.e., H mice have increased milk energy output (MEO) and wean larger pups. Levels of oxidative damage were unchanged (liver) or reduced (brain and serum) in R versus N mice, and no differences in multiple measures of oxidative protection were found between H and L mice in liver (except for Glutathione Peroxidase), brain or mammary glands. Also, there were no associations between an individual's energetic investment (e.g., MEO) and most of the oxidative stress measures detected in various tissues. These data are inconsistent with the oxidative stress theory, but were more supportive of, but not completely consistent, with the 'oxidative shielding' hypothesis.
authors.affiliation: Institute of Biological and Environmental Sciences, University of Aberdeen, Aberdeen AB24 2TZ, UK.|Department of Physiology and Pharmacology, College of Veterinary Medicine, University of Karbala, Karbala, Iraq.
authors.forename: Aqeel H Al
authors.initials: AH
authors.lastname: Jothery
authors.affiliation: Institute of Biological and Environmental Sciences, University of Aberdeen, Aberdeen AB24 2TZ, UK.
authors.forename: Lobke M
authors.initials: LM
authors.lastname: Vaanholt
authors.affiliation: Institute of Medical Sciences, University of Aberdeen, College of Life Sciences and Medicine, Foresterhill Health Campus, Aberdeen, United Kingdom.
authors.forename: Nimesh
authors.initials: N
authors.lastname: Mody
authors.affiliation: Section of Experimental Animal Models, Faculty of Health &Medical Sciences,University of Copenhagen, Denmark.
authors.forename: Anis
authors.initials: A
authors.lastname: Arnous
authors.affiliation: Section of Experimental Animal Models, Faculty of Health &Medical Sciences,University of Copenhagen, Denmark.
authors.forename: Jens
authors.initials: J
authors.lastname: Lykkesfeldt
authors.affiliation: Animal and Veterinary Science Group, Scotland's Rural College (SRUC), Edinburgh EH9 3JG, UK.
authors.forename: Lutz
authors.initials: L
authors.lastname: Bünger
authors.affiliation: Institute of Evolutionary Biology, University of Edinburgh, Edinburgh EH9 3JT, UK.
authors.forename: William G
authors.initials: WG
authors.lastname: Hill
authors.affiliation: Institute of Biological and Environmental Sciences, University of Aberdeen, Aberdeen AB24 2TZ, UK.
authors.forename: Sharon E
authors.initials: SE
authors.lastname: Mitchell
authors.affiliation: School of Public Health, University of Alabama at Birmingham, Birmingham, Alabama, USA.
authors.forename: David B
authors.initials: DB
authors.lastname: Allison
authors.affiliation: Institute of Biological and Environmental Sciences, University of Aberdeen, Aberdeen AB24 2TZ, UK.|Institute of Genetics and Developmental Biology, State Key Laboratory of Molecular Developmental Biology, Chinese Academy of Sciences, Beijing, People's Republic of China.
authors.forename: John R
authors.initials: JR
authors.lastname: Speakman
mesh_terms: D000818:Animals; D004435:Eating / Q000502:physiology*; D005260:Female; D008297:Male; D051379:Mice; D010084:Oxidation-Reduction; D012098:Reproduction / Q000502:physiology*