title: A proteomic adaptation of small intestinal mucosa in response to dietary protein limitation.
abstract: Dietary protein limitation (PL) is not only beneficial to human health but also applied to minimize nitrogen excretion in livestock production. However, the impact of PL on intestinal physiology is largely unknown. In this study, we identified 5275 quantitative proteins using a porcine model in which pigs suffered PL. A total of 202 proteins |log2fold-change| > 1 were taken as differentially expressed proteins and subjected to functional and pathway enrichment analysis to reveal proteomic alterations of the jejunal mucosa. Combining with the results of western blotting analysis, we found that protein/carbohydrate digestion, intestinal mucosal tight junction and cell adhesion molecules, and the immune response to foreign antigens were increased in the jejunal mucosa of the pigs upon PL. In contrast, amino acid transport, innate and auto immunity, as well as cell proliferation and apoptosis were reduced. In addition, the expression of functional proteins that involved in DNA replication, transcription and mRNA splicing as well as translation were altered in the jejunal mucosa in response to PL. Furthermore, PL may reduce amino acid transport and cell proliferation through the depression of mTOR pathway. This study provides new insights into the molecular mechanisms underlying the small intestinal response to PL.
authors.affiliation: State Key Lab of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing, 100193, P. R. China.
authors.forename: Chunfu
authors.initials: C
authors.lastname: Qin
authors.affiliation: State Key Lab of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing, 100193, P. R. China.
authors.forename: Kai
authors.initials: K
authors.lastname: Qiu
authors.affiliation: State Key Lab of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing, 100193, P. R. China.
authors.forename: Wenjuan
authors.initials: W
authors.lastname: Sun
authors.affiliation: State Key Lab of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing, 100193, P. R. China.
authors.forename: Ning
authors.initials: N
authors.lastname: Jiao
authors.affiliation: State Key Lab of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing, 100193, P. R. China.
authors.forename: Xin
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Institute of Animal Nutrition, Sichuan Agricultural University, Ya'an, 625099, P. R. China.
authors.forename: Lianqiang
authors.initials: L
authors.lastname: Che
authors.affiliation: Genecreate Biological Engineering Co., Ltd., National Bio-industry Base, Wuhan, 430075, P. R. China.
authors.forename: Haiyi
authors.initials: H
authors.lastname: Zhao
authors.affiliation: Genecreate Biological Engineering Co., Ltd., National Bio-industry Base, Wuhan, 430075, P. R. China.
authors.forename: Hexiao
authors.initials: H
authors.lastname: Shen
authors.affiliation: State Key Lab of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing, 100193, P. R. China.
authors.forename: Jingdong
authors.initials: J
authors.lastname: Yin
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D015551:Autoimmunity / Q000187:drug effects; D050260:Carbohydrate Metabolism; D015815:Cell Adhesion Molecules / Q000187:drug effects / Q000378:metabolism; D004044:Dietary Proteins / Q000009:adverse effects*; D005786:Gene Expression Regulation / Q000187:drug effects; D053263:Gene Regulatory Networks / Q000187:drug effects*; D007413:Intestinal Mucosa / Q000378:metabolism*; D007421:Intestine, Small / Q000378:metabolism*; D008297:Male; D040901:Proteomics / Q000379:methods*; D013552:Swine; D062725:Tight Junction Proteins / Q000187:drug effects / Q000378:metabolism
chemical_list: D015815:Cell Adhesion Molecules; D004044:Dietary Proteins; D062725:Tight Junction Proteins