title: Effect of boiling on the antidiabetic property of enzyme treated sheep milk casein.
abstract: OBJECTIVE
  Sheep milk-born bioactive peptides have been found to exhibit various physiological activities. The present work was designed with the aim to evaluate the effect of boiling on antidiabetic property of peptides derived from sheep milk caseinate on hydrolysis with three different proteases.
  
  
  METHODS
  In this investigation, casein prepared from raw and boiled sheep milk was hydrolyzed by three commercially available proteases (trypsin, pepsin, and chymotrypsin). These hydrolysates collected at different hydrolysis times (60, 120, 160, and 240 min) were assayed for their antidiabetic activity.
  
  
  RESULTS
  Among the three different enzyme hydrolysates, casein treated with chymotrypsin shows the highest antidiabetic activity among other enzymes. While the antidiabetic effect of raw milk-derived casein hydrolysates always exceeds than effect shown by boiled milk casein hydrolysates.
  
  
  CONCLUSIONS
  The result obtained hence shows that the effect of boiling on the properties of bioactive peptides released during different enzyme digestion depends largely on the enzymatic formulation used and treatment conditions. Chymotrypsin treatment of raw casein yields peptides with maximum antidiabetic activity as compared to pepsin and trypsin. Moreover, the peptides produced after enzymatic treatment of boiled casein show reduced antidiabetic properties. Therefore, enzymatically treated raw milk casein hydrolysates may be used as effective nutritional supplements for diabetic patients, as it causes a significant inhibition of α-amylase activity.
authors.affiliation: Department of Biochemistry, Dolphin PG Institute of Biomedical & Natural Sciences, Dehradun, Uttarakhand, India.
authors.forename: Farhath
authors.initials: F
authors.lastname: Jan
authors.affiliation: Department of Biotechnology, Uttranchal University, Dehradun, Uttarakhand, India.
authors.forename: Santosh
authors.initials: S
authors.lastname: Kumar
authors.affiliation: Department of Biotechnology, Uttranchal University, Dehradun, Uttarakhand, India.
authors.forename: Richa
authors.initials: R
authors.lastname: Jha
keywords: antidiabetic; bioactive peptides; casein; hydrolysates; proteases; α-amylase