title: Centromere protein W interacts with beta-transducin repeat-containing protein 1 and modulates its subcellular localization.
abstract: Beta-transducin repeat-containing protein 1 (β-TrCP1) is a substrate-recognition module of SCFβ-TrCP1ubiquitin ligases and its subcellular distribution is known to be critical for target specificity. Heterogeneous nuclear ribonucleoprotein (hnRNP) U, an abundant nuclear protein, is known to be a unique regulator of β-TrCP1 shuttling between the cytoplasm and the nucleus. In this study, we report that centromere protein W (CENP-W), which is frequently overexpressed in a variety of human cancers, may also contribute to β-TrCP1 shuttling. Although hnRNP U and CENP-W can interact with β-TrCP1 and transport it independently, these proteins do not compete for β-TrCP1 binding, but rather cooperate to form a stable shuttling complex. Intriguingly, we found that overexpression of CENP-W leads to accumulation of β-TrCP1 in the nucleus. Thus, we propose that CENP-W may function as a booster of β-TrCP1 nuclear import to increase the oncogenicity of β-TrCP1.
authors.affiliation: Department of Microbiology and Molecular Biology, Chungnam National University, Daejeon, Korea.
authors.forename: Yeongmi
authors.initials: Y
authors.lastname: Cheon
authors.affiliation: Department of Microbiology and Molecular Biology, Chungnam National University, Daejeon, Korea.
authors.forename: Seyeong
authors.initials: S
authors.lastname: Jeon
authors.affiliation: Department of Microbiology and Molecular Biology, Chungnam National University, Daejeon, Korea.
authors.forename: Soojin
authors.identifier: http://orcid.org/0000-0002-0383-5025
authors.initials: S
authors.lastname: Lee
mesh_terms: D021581:Active Transport, Cell Nucleus / Q000235:genetics; D002467:Cell Nucleus / Q000378:metabolism* / Q000648:ultrastructure; D002503:Centromere / Q000378:metabolism* / Q000648:ultrastructure; D002868:Chromosomal Proteins, Non-Histone / Q000235:genetics / Q000378:metabolism*; D003593:Cytoplasm / Q000378:metabolism* / Q000648:ultrastructure; D005786:Gene Expression Regulation; D057809:HEK293 Cells; D034722:Heterogeneous-Nuclear Ribonucleoprotein U / Q000235:genetics / Q000378:metabolism; D006801:Humans; D010957:Plasmids / Q000737:chemistry / Q000378:metabolism; D011485:Protein Binding; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction; D014162:Transfection; D044844:beta-Transducin Repeat-Containing Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: C471644:BTRC protein, human; C534000:CENPW protein, human; D002868:Chromosomal Proteins, Non-Histone; D034722:Heterogeneous-Nuclear Ribonucleoprotein U; D011994:Recombinant Proteins; D044844:beta-Transducin Repeat-Containing Proteins
keywords: cancer; centromere protein W; cullin-RING ubiquitin ligase; nuclear export; nuclear import; β-TrCP1