title: Two MYB-related transcription factors play opposite roles in sugar signaling in Arabidopsis.
abstract: Sugar regulation of gene expression has profound effects at all stages of the plant life cycle. Although regulation at the transcriptional level is one of the most prominent mechanisms by which gene expression is regulated, only a few transcription factors have been identified and demonstrated to be involved in the regulation of sugar-regulated gene expression. OsMYBS1, an R1/2-type MYB transcription factor, has been demonstrated to be involved in sugar- and hormone-regulated α-amylase gene expression in rice. Arabidopsis contains two OsMYBS1 homologs. In the present study, we investigate MYBS1 and MYBS2 in sugar signaling in Arabidopsis. Our results indicate that MYBS1 and MYBS2 play opposite roles in regulating glucose and ABA signaling in Arabidopsis during seed germination and early seedling development. MYB proteins have been classified into four subfamilies: R2R3-MYB, R1/2-MYB, 3R-MYB, and 4R-MYB. An R1/2-type MYB transcription factor, OsMYBS1, has been demonstrated to be involved in sugar- and hormone-regulated α-amylase genes expression in rice. In this study, two genes homologous to OsMYBS1, MYBS1 and MYBS2, were investigated in Arabidopsis. Subcellular localization analysis showed that MYBS1 and MYBS2 were localized in the nucleus. Rice embryo transient expression assays indicated that both MYBS1 and MYBS2 could recognize the sugar response element, TA-box, in the promoter and induced promoter activity. mybs1 mutant exhibited hypersensitivity to glucose, whereas mybs2 seedlings were hyposensitive to it. MYBS1 and MYBS2 are involved in the control of glucose-responsive gene expression, as the mybs1 mutant displayed increased expression of a hexokinase gene (HXK1), chlorophyll a/b-binding protein gene (CAB1), ADP-glucose pyrophosphorylase gene (APL3), and chalcone synthase gene (CHS), whereas the mybs2 mutant exhibited decreased expression of these genes. mybs1 also showed an enhanced response to abscisic acid (ABA) in the seed germination and seedling growth stages, while mybs2 showed reduced responses. The ABA biosynthesis inhibitor fluridone rescued the mybs1 glucose-hypersensitive phenotype. Moreover, the mRNA levels of three ABA biosynthesis genes, ABA1, NCED9, and AAO3, and three ABA signaling genes, ABI3, ABI4, and ABI5, were increased upon glucose treatment of mybs1 seedlings, but were decreased in mybs2 seedlings. These results indicate that MYBS1 and MYBS2 play opposite roles in regulating glucose and ABA signaling in Arabidopsis during seed germination and early seedling development.
authors.affiliation: Department of Life Sciences, National Central University, Jhongli District, Taoyuan City, 32001, Taiwan.
authors.forename: Yi-Shih
authors.initials: YS
authors.lastname: Chen
authors.affiliation: Department of Life Sciences, National Central University, Jhongli District, Taoyuan City, 32001, Taiwan.
authors.forename: Yi-Chi
authors.initials: YC
authors.lastname: Chao
authors.affiliation: Department of Life Sciences, National Central University, Jhongli District, Taoyuan City, 32001, Taiwan.
authors.forename: Tzu-Wei
authors.initials: TW
authors.lastname: Tseng
authors.affiliation: Department of Life Sciences, National Central University, Jhongli District, Taoyuan City, 32001, Taiwan.
authors.forename: Chun-Kai
authors.initials: CK
authors.lastname: Huang
authors.affiliation: Department of Life Sciences, National Central University, Jhongli District, Taoyuan City, 32001, Taiwan.
authors.forename: Pei-Ching
authors.initials: PC
authors.lastname: Lo
authors.affiliation: Department of Life Sciences, National Central University, Jhongli District, Taoyuan City, 32001, Taiwan. chungan@cc.ncu.edu.tw.
authors.forename: Chung-An
authors.initials: CA
authors.lastname: Lu
mesh_terms: D000040:Abscisic Acid / Q000494:pharmacology; D017360:Arabidopsis / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D050260:Carbohydrate Metabolism / Q000187:drug effects / Q000235:genetics; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D017343:Genes, Plant; D005816:Genetic Complementation Test; D018525:Germination / Q000187:drug effects; D005947:Glucose / Q000494:pharmacology; D012275:Oryza / Q000196:embryology / Q000235:genetics; D030821:Plants, Genetically Modified; D011401:Promoter Regions, Genetic; D012639:Seeds / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects / Q000235:genetics; D013347:Subcellular Fractions / Q000187:drug effects / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*
chemical_list: D029681:Arabidopsis Proteins; C000614450:MYBS1 protein, Arabidopsis; C000614451:MYBS2 protein, Arabidopsis; D014157:Transcription Factors; D000040:Abscisic Acid; D005947:Glucose
keywords: ABA; Arabidopsis; MYB transcription factor; Sugar signaling