title: Detection and Genotyping of Human Papillomaviruses from Archival Formalin-Fixed Tissue Samples.
abstract: Pathology departments routinely process and store formalin-fixed, paraffin-embedded (FFPE) tissue samples for clinical diagnosis. These collections often contain decades' worth of samples and represent a treasure trove of specimens that can be analyzed for retrospective epidemiological studies, diagnostics, and pathogen discovery. Accurate amplification and sequencing of DNA from these samples is critical for the usability of these FFPE samples. Here we present a collection of protocols that describe extraction of DNA from FFPE tissues, PCR amplification of human papillomavirus DNA, and subsequent genotyping of the infecting virus. © 2016 by John Wiley & Sons, Inc.
authors.affiliation: Lab of Viral Diseases, NIAID, NIH, Bethesda, Maryland.
authors.forename: Koenraad
authors.initials: K
authors.lastname: Van Doorslaer
authors.affiliation: Department of Microbiology, The Chinese University of Hong Kong, Hong Kong.
authors.forename: Zigui
authors.initials: Z
authors.lastname: Chen
authors.affiliation: Lab of Viral Diseases, NIAID, NIH, Bethesda, Maryland.
authors.forename: Alison A
authors.initials: AA
authors.lastname: McBride
mesh_terms: D003001:Cloning, Molecular / Q000379:methods*; D004279:DNA, Viral / Q000235:genetics; D005838:Genotype; D006801:Humans; D027383:Papillomaviridae / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D030361:Papillomavirus Infections / Q000821:virology*; D016133:Polymerase Chain Reaction / Q000379:methods*; D016610:Tissue Embedding / Q000379:methods*; D016707:Tissue Fixation / Q000379:methods*
chemical_list: D004279:DNA, Viral
keywords: FFPE; HPV; PCR; cancer; cervix; degenerate primers; formalin; neoplasia; papillomavirus; sequencing