title: Quantification of furosemide in camel plasma by high resolution mass spectrometry, application on pharmacokinetics.
abstract: We developed and validated a high-resolution liquid chromatography mass spectrometry method for the quantification of furosemide in camel plasma which was used for a pharmacokinetic study in camels. Plasma samples were extracted by supported liquid extraction and furosemide and internal standard (furosemide-D5) were separated on a an Agilent Zorbax XDB C18column (50 × 2.1 mm i.d., 3.5 μm). Data was acquired in full-scan mode over a mass range of 200-400 Da in negative electrospray mode at a resolution of 70,000. Linear calibration curves were obtained over the concentration ranges of 1.0-10,000 ng/mL. The validated method was then successfully applied in evaluating the pharmacokinetics and metabolites of furosemide in six camels (Camelus dromedarus) and we were able to advice on a withdrawal time of furosemide treatment before racing.
authors.affiliation: Camel Racing Labosratory, Forensic Evidence Department, Abu Dhabi, United Arab Emirates.
authors.forename: Ibrahim A
authors.initials: IA
authors.lastname: Wasfi
authors.affiliation: Camel Racing Labosratory, Forensic Evidence Department, Abu Dhabi, United Arab Emirates.
authors.forename: Sayed A
authors.initials: SA
authors.lastname: Wajid
authors.affiliation: Camel Racing Labosratory, Forensic Evidence Department, Abu Dhabi, United Arab Emirates.
authors.forename: B A
authors.initials: BA
authors.lastname: Agha
authors.affiliation: Camel Racing Labosratory, Forensic Evidence Department, Abu Dhabi, United Arab Emirates.
authors.forename: Asmaa M
authors.initials: AM
authors.lastname: Kamel
authors.affiliation: Camel Racing Labosratory, Forensic Evidence Department, Abu Dhabi, United Arab Emirates.
authors.forename: Nasreen A
authors.initials: NA
authors.lastname: Al Biriki
authors.affiliation: Camel Racing Labosratory, Forensic Evidence Department, Abu Dhabi, United Arab Emirates.
authors.forename: Khaled M
authors.initials: KM
authors.lastname: Al Neaimi
authors.affiliation: Camel Racing Labosratory, Forensic Evidence Department, Abu Dhabi, United Arab Emirates.
authors.forename: Waleed A
authors.initials: WA
authors.lastname: Al Ali
mesh_terms: D000818:Animals; D002162:Camelus; D005665:Furosemide / Q000097:blood* / Q000493:pharmacokinetics; D008297:Male; D013058:Mass Spectrometry / Q000379:methods*; D012015:Reference Standards
chemical_list: D005665:Furosemide
keywords: camels; clearance; furosemide; high-resolution; pharmacokinetics