title: Fibroblast growth factor 10 markedly improves in vitro maturation of porcine cumulus-oocyte complexes.
abstract: Growth factors synthesized by ovarian somatic cells affect cumulus cell expansion and oocyte maturation in vitro. Fibroblast growth factor 10 (FGF10), for example, is a known regulator of mammalian cumulus-oocyte complex maturation. In this study, we investigated the effects of 0, 5, 10, 50, and 100 ng/mL FGF10 (5F, 10F, 50F, and 100F, respectively) on in vitro cumulus cell expansion, oocyte maturation, and embryo development. The percentage of fully expanded cumulus cells at the oocyte's metaphase-II (MII) stage was significantly higher in the 10F-treated group than in the control. Transcript abundance of the cumulus cell expansion-related gene encoding hyaluronian synthase 2 (HAS2) in cumulus cells at oocyte germinal vesicle breakdown (GVBD) was significantly higher in the 10F- and 50F-treated groups compared to untreated controls, whereas the mRNA abundance of the protease cathepsin B (CTSB) at the oocyte MII stage was remarkably decreased in the 10F-treated group. The percentage of oocytes with normal spindles was greater in the 10F- and 50F-treated group at GVBD than in the other groups; the 5F-, 10F-, and 100F-treated groups were higher than the control; and the 50F-treated group was highest at MII. The abundance of GDF9 and BMP15 transcript at GVBD and BMP15 and CCNB1 transcripts at MII increased in the 10F-treated group. Cleavage rate, blastocyst formation rate, and total cell number were significantly higher in the 5F- to 50F-treated groups. These results demonstrate that FGF10 markedly improves cumulus cell expansion, oocyte maturation, and subsequent embryo development. Mol. Reprod. Dev. 84: 67-75, 2017. © 2016 Wiley Periodicals, Inc.
authors.affiliation: Faculty of Biotechnology, College of Applied Life Sciences, Jeju National University, Jeju, Jeju Special Self-Governing Province, Korea.|Stem Cell Research Center, Jeju National University, Jeju, Jeju Special Self-Governing Province, Korea.
authors.forename: Yeo-Jin
authors.initials: YJ
authors.lastname: Son
authors.affiliation: Faculty of Biotechnology, College of Applied Life Sciences, Jeju National University, Jeju, Jeju Special Self-Governing Province, Korea.|Stem Cell Research Center, Jeju National University, Jeju, Jeju Special Self-Governing Province, Korea.
authors.forename: Seung-Eun
authors.initials: SE
authors.lastname: Lee
authors.affiliation: Faculty of Biotechnology, College of Applied Life Sciences, Jeju National University, Jeju, Jeju Special Self-Governing Province, Korea.|Stem Cell Research Center, Jeju National University, Jeju, Jeju Special Self-Governing Province, Korea.
authors.forename: Hyuk
authors.initials: H
authors.lastname: Hyun
authors.affiliation: Faculty of Biotechnology, College of Applied Life Sciences, Jeju National University, Jeju, Jeju Special Self-Governing Province, Korea.|Stem Cell Research Center, Jeju National University, Jeju, Jeju Special Self-Governing Province, Korea.
authors.forename: Min-Young
authors.initials: MY
authors.lastname: Shin
authors.affiliation: Faculty of Biotechnology, College of Applied Life Sciences, Jeju National University, Jeju, Jeju Special Self-Governing Province, Korea.|Stem Cell Research Center, Jeju National University, Jeju, Jeju Special Self-Governing Province, Korea.
authors.forename: Yun-Gwi
authors.initials: YG
authors.lastname: Park
authors.affiliation: Faculty of Biotechnology, College of Applied Life Sciences, Jeju National University, Jeju, Jeju Special Self-Governing Province, Korea.|Stem Cell Research Center, Jeju National University, Jeju, Jeju Special Self-Governing Province, Korea.
authors.forename: Sang-Gi
authors.initials: SG
authors.lastname: Jeong
authors.affiliation: Faculty of Biotechnology, College of Applied Life Sciences, Jeju National University, Jeju, Jeju Special Self-Governing Province, Korea.|Stem Cell Research Center, Jeju National University, Jeju, Jeju Special Self-Governing Province, Korea.|Mirae Cell Bio, Gwangjin-Gu, Seoul, Korea.
authors.forename: Eun-Young
authors.initials: EY
authors.lastname: Kim
authors.affiliation: Faculty of Biotechnology, College of Applied Life Sciences, Jeju National University, Jeju, Jeju Special Self-Governing Province, Korea.|Stem Cell Research Center, Jeju National University, Jeju, Jeju Special Self-Governing Province, Korea.|Mirae Cell Bio, Gwangjin-Gu, Seoul, Korea.
authors.forename: Se-Pill
authors.initials: SP
authors.lastname: Park
mesh_terms: D000818:Animals; D055430:Bone Morphogenetic Protein 15 / Q000096:biosynthesis; D002401:Cathepsin B / Q000096:biosynthesis; D002478:Cells, Cultured; D054885:Cumulus Cells / Q000166:cytology / Q000378:metabolism*; D005260:Female; D051526:Fibroblast Growth Factor 10 / Q000494:pharmacology*; D055429:Growth Differentiation Factor 9 / Q000096:biosynthesis; D000076002:Hyaluronan Synthases / Q000096:biosynthesis; D009865:Oocytes / Q000166:cytology / Q000378:metabolism*; D013552:Swine
chemical_list: D055430:Bone Morphogenetic Protein 15; D051526:Fibroblast Growth Factor 10; D055429:Growth Differentiation Factor 9; D000076002:Hyaluronan Synthases; D002401:Cathepsin B