title: Elevated expression of STIM1 is involved in lung tumorigenesis.
abstract: This study aimed to address the potential role of STIM1 (stromal interaction molecule 1) in lung tumorigenesis. Colony formation in soft agar assay and tumorigenicity in nude mice assay were conducted. Western blot, immunohistochemistry and quantitative real-time polymerase chain reaction were used to measure the STIM1 expression. The distribution of cell cycle was detected by flow cytometry assay. Our results showed that the expression of STIM1 mRNA was significantly higher in human lung tumors than that in adjacent non-neoplastic lung tissues. Significantly increased expression of STIM1 mRNA and protein was observed in 16HBE-benzo(a)pyrene (BaP) cells and in BaP-treated mice lung tissues compared with 16HBE-control cells and the control group, respectively. Silencing STIM1 inhibited the proliferation and colony formation of A549 cells in in vitro experiments, attenuated the growth of tumor xenografts of A549 cells in in vivo experiments and induced the arrest of cell cycle in the G1 phase. The markedly decreased expression of cyclin D1 protein was observed in A549-shRNA-STIM1 cells as compared to A549-shRNA-control cells. The markedly increased expression of p21 protein was observed in A549-shRNA-STIM1 cells as compared to A549-shRNA-control cells. The expression levels of β-catenin and TGIF proteins were lower in A549-shRNA-STIM1 cells than those in A549-shRNA-control cells. In conclusion, this study indicated that the elevated expression of STIM1 might be involved in lung tumorigenesis.
authors.affiliation: Department of Toxicology, Henan Center for Disease Control and Prevention, Zhengzhou 450016, China.|Henan Collaborative Innovation Center of Molecular Diagnosis and Laboratory Medicine, Xinxiang Medical University, Xinxiang 453003, China.
authors.forename: Yadong
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Toxicology, Henan Center for Disease Control and Prevention, Zhengzhou 450016, China.
authors.forename: Haiyu
authors.initials: H
authors.lastname: Wang
authors.affiliation: Department of Toxicology, Henan Center for Disease Control and Prevention, Zhengzhou 450016, China.
authors.forename: Li
authors.initials: L
authors.lastname: Li
authors.affiliation: Department of Toxicology, Henan Center for Disease Control and Prevention, Zhengzhou 450016, China.
authors.forename: Jiangmin
authors.initials: J
authors.lastname: Li
authors.affiliation: Department of Epidemiology, School of Public Health, Zhengzhou University, Zhengzhou 450001, China.
authors.forename: Teng
authors.initials: T
authors.lastname: Pan
authors.affiliation: Department of Toxicology, Henan Center for Disease Control and Prevention, Zhengzhou 450016, China.
authors.forename: Ding
authors.initials: D
authors.lastname: Zhang
authors.affiliation: Department of Epidemiology, School of Public Health, Zhengzhou University, Zhengzhou 450001, China.
authors.forename: Haiyan
authors.initials: H
authors.lastname: Yang
mesh_terms: D033942:Adaptor Proteins, Vesicular Transport / Q000032:analysis; D000818:Animals; D001564:Benzo(a)pyrene / Q000633:toxicity; D063646:Carcinogenesis; D045744:Cell Line, Tumor; D005260:Female; D059585:G1 Phase Cell Cycle Checkpoints; D006801:Humans; D008175:Lung Neoplasms / Q000737:chemistry / Q000209:etiology*; D051379:Mice; D008807:Mice, Inbred BALB C; D009363:Neoplasm Proteins / Q000032:analysis / Q000502:physiology*; D000071737:Stromal Interaction Molecule 1 / Q000032:analysis / Q000502:physiology*; D051176:beta Catenin / Q000032:analysis
chemical_list: D033942:Adaptor Proteins, Vesicular Transport; C495270:CTNNB1 protein, human; D009363:Neoplasm Proteins; C103530:STIM1 protein, human; D000071737:Stromal Interaction Molecule 1; C470409:TICAM1 protein, human; D051176:beta Catenin; D001564:Benzo(a)pyrene
keywords: A549 cell; STIM1; cell cycle; lung cancer; tumorigenicity