title: Influence of Incubation Conditions on Biofilm Formation byPseudomonas FluorescensIsolated from Dairy Products and Dairy Manufacturing Plants.
abstract: In this study, biofilm formation of 64Pseudomonas fluorescensstrains isolated from milk, dairy products and dairy plants was compared. The strains were grown on Tryptic Soy Broth supplemented with 0.2% of glucose, on polystyrene microplates at 10 and 30°C for 48 h. In general, 57/64P. fluorescensstrains formed biofilm, although with great variability at both tested temperatures. Moreover, our results evidenced that the biofilm-forming ability of the strains was temperature- and strain-dependent. Interestingly, the ability of several isolates to form biofilms was associated with the low temperature after 48 h. Our findings evidenced that temperature was more important than incubation time for biofilm formation. Considering the origin of the strains, it is relevant to underline the importance of performing accurate cleaning and disinfection procedures on food processing surfaces.
authors.affiliation: Faculty of Bioscience and Technology for Food, Agriculture and Environment, University of Teramo , Mosciano S. Angelo (TE), Italy.
authors.forename: Chiara
authors.initials: C
authors.lastname: Rossi
authors.affiliation: Faculty of Bioscience and Technology for Food, Agriculture and Environment, University of Teramo , Mosciano S. Angelo (TE), Italy.
authors.forename: Clemencia
authors.initials: C
authors.lastname: Chaves-López
authors.affiliation: Faculty of Bioscience and Technology for Food, Agriculture and Environment, University of Teramo , Mosciano S. Angelo (TE), Italy.
authors.forename: Annalisa
authors.initials: A
authors.lastname: Serio
authors.affiliation: Veterinary Public Health Institute of Apulia and Basilicata , Foggia, Italy.
authors.forename: Elisa
authors.initials: E
authors.lastname: Goffredo
authors.affiliation: Department of Veterinary Medicine, University of Perugia , Perugia, Italy.
authors.forename: Beniamino Terzo Cenci
authors.initials: BT
authors.lastname: Goga
authors.affiliation: Faculty of Bioscience and Technology for Food, Agriculture and Environment, University of Teramo , Mosciano S. Angelo (TE), Italy.
authors.forename: Antonello
authors.initials: A
authors.lastname: Paparella
keywords: Biofilm; Mozzarella cheese; Pseudomonas fluorescens; Dairy products