title: Alterations of gene expression indicating effects on estrogen signaling and lipid homeostasis in seabream hepatocytes exposed to extracts of seawater sampled from a coastal area of the central Adriatic Sea (Italy).
abstract: Recent evidences suggest that the toxicological effects of endocrine disrupting chemicals (EDCs) involve multiple nuclear receptor-mediated pathways, including estrogen receptor (ER) and peroxisome proliferator-activated receptor (PPAR) signaling systems. Thus, our objective in this study was to detect the summated endocrine effects of EDCs with metabolic activity in coastal waters of the central Adriatic Sea by means of a toxicogenomic approach using seabream hepatocytes. Gene expression patterns were also correlated with seawater levels of polychlorinated biphenyls (PCBs) and polycyclic aromatic hydrocarbons (PAHs). We found that seawater extracts taken at certain areas induced gene expression profiles of ERα/vitellogenin, PPARα/Stearoyl-CoA desaturase 1A, cytochrome P4501A (CYP1A) and metallothionein. These increased levels of biomarkers responses correlated with spatial distribution of PAHs/PCBs concentrations observed by chemical analysis in the different study areas. Collectively, our data give a snapshot of the presence of complex EDC mixtures that are able to perturb metabolic signaling in coastal marine waters.
authors.affiliation: School of Biosciences and Veterinary Medicine, University of Camerino, Via Gentile III Da Varano, I-62032 Camerino MC, Italy.
authors.forename: Paolo
authors.initials: P
authors.lastname: Cocci
authors.affiliation: School of Biosciences and Veterinary Medicine, University of Camerino, Via Gentile III Da Varano, I-62032 Camerino MC, Italy.
authors.forename: Martina
authors.initials: M
authors.lastname: Capriotti
authors.affiliation: School of Biosciences and Veterinary Medicine, University of Camerino, Via Gentile III Da Varano, I-62032 Camerino MC, Italy.
authors.forename: Gilberto
authors.initials: G
authors.lastname: Mosconi
authors.affiliation: National Research Council, Institute of Marine Science CNR-ISMAR, L.go Fiera della Pesca, 2, 60125 Ancona, Italy.
authors.forename: Alessandra
authors.initials: A
authors.lastname: Campanelli
authors.affiliation: National Research Council, Institute of Marine Science CNR-ISMAR, L.go Fiera della Pesca, 2, 60125 Ancona, Italy.
authors.forename: Emanuela
authors.initials: E
authors.lastname: Frapiccini
authors.affiliation: National Research Council, Institute of Marine Science CNR-ISMAR, L.go Fiera della Pesca, 2, 60125 Ancona, Italy.
authors.forename: Mauro
authors.initials: M
authors.lastname: Marini
authors.affiliation: School of Pharmacy, University of Camerino, Via Sant'Agostino 1, I-62032 Camerino MC, Italy.
authors.forename: Giovanni
authors.initials: G
authors.lastname: Caprioli
authors.affiliation: School of Pharmacy, University of Camerino, Via Sant'Agostino 1, I-62032 Camerino MC, Italy.
authors.forename: Gianni
authors.initials: G
authors.lastname: Sagratini
authors.affiliation: Controllo Statistico, Pescara, Italy(1); Marine Protected Area Torre del Cerrano, 64025 Pineto, TE, Italy.
authors.forename: Graziano
authors.initials: G
authors.lastname: Aretusi
authors.affiliation: School of Biosciences and Veterinary Medicine, University of Camerino, Via Gentile III Da Varano, I-62032 Camerino MC, Italy. Electronic address: francesco.palermo@unicam.it.
authors.forename: Francesco Alessandro
authors.initials: FA
authors.lastname: Palermo
mesh_terms: D000818:Animals; D052244:Endocrine Disruptors / Q000633:toxicity; D004784:Environmental Monitoring / Q000379:methods*; D015870:Gene Expression / Q000187:drug effects; D022781:Hepatocytes / Q000187:drug effects / Q000502:physiology*; D006706:Homeostasis; D007558:Italy; D008055:Lipids / Q000502:physiology*; D011078:Polychlorinated Biphenyls / Q000633:toxicity; D011084:Polycyclic Aromatic Hydrocarbons / Q000633:toxicity; D021541:Sea Bream; D012623:Seawater; D014874:Water Pollutants, Chemical / Q000633:toxicity*
chemical_list: D052244:Endocrine Disruptors; D008055:Lipids; D011084:Polycyclic Aromatic Hydrocarbons; D014874:Water Pollutants, Chemical; D011078:Polychlorinated Biphenyls
keywords: Adriatic Sea; Coastal waters; Endocrine disruption; Gene transcription; Oestrogen mimics; Sparus aurata