title: An investigation into respiratory tract viruses in children with acute lower respiratory tract infection or wheezing.
abstract: BACKGROUND
  This study aimed to determine the frequencies of respiratory tract viruses in patient (acute lower respiratory tract infection [LRTI] or wheezing) and control (history of asthma without symptoms) groups.
  
  
  METHODS
  Using multiplex-polymerase chain reaction (PCR), respiratory tract viruses were investigated in the respiratory tract specimens from patient and control groups followed in the Pediatric Clinic.
  
  
  RESULTS
  The viruses detected in the patient and control groups (P=0.013) were as follows, respectively: rhinoviruses A, B, C (25.6% and 36.7%), influenza virus A (21.1% and 0.0%), parainfluenza virus type 1 (7.8% and 1.7%), parainfluenza virus type 4 (5.6% and 0.0%), adenoviruses A, B, C, D, E (4.4% and 1.7%), parainfluenza virus type 3 (4.4% and 1.7%), coronaviruses 229E and NL63 (4.4% and 1.7%), coronavirus OC43 (3.3% and 0.0%), respiratory syncytial virus A (3.3% and 0.0%), parainfluenza virus type 2 (2.2% and 0.0%), influenza virus B (2.2% and 0.0%), and respiratory syncytial virus B (1.1% and 1.7%). No bocavirus, metapneumovirus or enterovirus was found in any specimen. Statistically significant differences in the detection of influenza virus A (P=0.000), the total detection of parainfluenza viruses (P=0.008) and coinfection (P=0.004) were observed between the patient and control groups.
  
  
  CONCLUSIONS
  The advantage of our study compared with other studies is the inclusion of not only wheezing patients but also children with asthma without symptom. The higher detection of rhinoviruses both in patient and control groups give rise to thought that these viruses may be responsible for asthma exacerbations and may be related with long duration of virus shedding.
authors.affiliation: Department of Medical Microbiology, Faculty of Medicine, Gaziantep University, Gaziantep, Turkey.
authors.forename: Demet
authors.initials: D
authors.lastname: Dabaniyasti
authors.affiliation: Department of Medical Microbiology, Faculty of Medicine, Gaziantep University, Gaziantep, Turkey - eksi@gantep.edu.tr.
authors.forename: Fahriye
authors.initials: F
authors.lastname: Eksi
authors.affiliation: Unit of Pediatric Allergy and Immunology, Faculty of Medicine, Gaziantep University, Gaziantep, Turkey.
authors.forename: Özlem
authors.initials: Ö
authors.lastname: Keskin
authors.affiliation: Unit of Pediatric Allergy and Immunology, Faculty of Medicine, Gaziantep University, Gaziantep, Turkey.
authors.forename: Mehmet Y
authors.initials: MY
authors.lastname: Özkars
authors.affiliation: Department of Medical Microbiology, Faculty of Medicine, Gaziantep University, Gaziantep, Turkey.
authors.forename: Tekin
authors.initials: T
authors.lastname: Karsligil
authors.affiliation: Department of Medical Microbiology, Faculty of Medicine, Gaziantep University, Gaziantep, Turkey.
authors.forename: Iclal
authors.initials: I
authors.lastname: Balci
mesh_terms: D000208:Acute Disease; D000293:Adolescent; D001249:Asthma; D016022:Case-Control Studies; D002648:Child; D002675:Child, Preschool; D017934:Coronavirus / Q000302:isolation & purification; D005260:Female; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D029521:Alphainfluenzavirus / Q000302:isolation & purification; D008297:Male; D060885:Multiplex Polymerase Chain Reaction; D012135:Respiratory Sounds; D018113:Respiratory Syncytial Virus, Human / Q000302:isolation & purification; D012137:Respiratory System / Q000821:virology*; D012141:Respiratory Tract Infections / Q000821:virology*; D018108:Respirovirus / Q000302:isolation & purification; D012229:Rhinovirus / Q000302:isolation & purification; D013048:Specimen Handling / Q000379:methods; D063189:Symptom Assessment