title: Pyruvate cellular uptake and enzymatic conversion probed by dissolution DNP-NMR: the impact of overexpressed membrane transporters.
abstract: Pyruvate membrane crossing and its lactate dehydrogenase-mediated conversion to lactate in cells featuring different levels of expression of membrane monocarboxylate transporters (MCT4) were probed by dissolution dynamic nuclear polarization-enhanced NMR. Hyperpolarized13C-1-labeled pyruvate was transferred to suspensions of rodent tumor cell carcinoma, cell line 39. The pyruvate-to-lactate conversion rate monitored by dissolution dynamic nuclear polarization-NMR in carcinoma cells featuring native MCT4 expression level was lower than the rate observed for cells in which the human MCT4 gene was overexpressed. The enzymatic activity of lactate dehydrogenase was also assessed in buffer solutions, following the real-time pyruvate-to-lactate conversion speeds at different enzyme concentrations. Copyright © 2016 John Wiley & Sons, Ltd.
authors.affiliation: Laboratoire de Chimie et Biochimie Pharmacologiques et Toxicologiques, UMR 8601, UFR Biomédicale et des Sciences de Base, Université Paris Descartes - CNRS, Paris, France.
authors.forename: Riccardo
authors.initials: R
authors.lastname: Balzan
authors.affiliation: Laboratoire de Chimie et Biochimie Pharmacologiques et Toxicologiques, UMR 8601, UFR Biomédicale et des Sciences de Base, Université Paris Descartes - CNRS, Paris, France.
authors.forename: Laetitia
authors.initials: L
authors.lastname: Fernandes
authors.affiliation: PARCC - Inserm U970 - Faculté de Médecine, Université Paris Descartes, Paris, France.
authors.forename: Laetitia
authors.initials: L
authors.lastname: Pidial
authors.affiliation: EPFL, Institute of Physics of Biological Systems, Lausanne, Switzerland.
authors.forename: Arnaud
authors.initials: A
authors.lastname: Comment
authors.affiliation: PARCC - Inserm U970 - Faculté de Médecine, Université Paris Descartes, Paris, France.
authors.forename: Bertrand
authors.initials: B
authors.lastname: Tavitian
authors.affiliation: Laboratoire de Chimie et Biochimie Pharmacologiques et Toxicologiques, UMR 8601, UFR Biomédicale et des Sciences de Base, Université Paris Descartes - CNRS, Paris, France.
authors.forename: Paul R
authors.initials: PR
authors.lastname: Vasos
mesh_terms: D000818:Animals; D001692:Biological Transport; D045744:Cell Line, Tumor; D006224:Cricetinae; D003412:Cricetulus; D006801:Humans; D019344:Lactic Acid / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D027501:Monocarboxylic Acid Transporters / Q000378:metabolism*; D019289:Pyruvic Acid / Q000378:metabolism*; D012995:Solubility
chemical_list: D027501:Monocarboxylic Acid Transporters; D019344:Lactic Acid; D019289:Pyruvic Acid
keywords: dissolution dynamic nuclear polarization (d-DNP); lactate dehydrogenase (LDH); metabolism; nuclear magnetic resonance (NMR); rodent cancer cells