title: Differences between renal effects of venom from two Bothrops jararaca populations from southeastern and southern Brazil.
abstract: Components from animal venoms may vary according to the snake's age, gender and region of origin. Recently, we performed a proteomic analysis of Bothrops jararaca venom from southern (BjSv) and southeastern (BjSEv) Brazil, showing differences in the venom composition, as well as its biological activity. To continue the study, we report in this short communication the different effects induced by the BjSEv and BjSv on isolated kidney and MDCK renal cells. BjSEv decreased perfusion pressure (PP) and renal vascular resistance (RVR) and increased urinary flow (UF) and glomerular filtration rate (GFR), while BjSv did not alter PP and RVR and reduced UF and GFR. Both types of venom, more expressively BjSEv, reduced %TNa+, %TK+and %Cl-. In MDCK cells, the two types of venom showed cytotoxicity with IC50of 1.22 μg/mL for BjSv and 1.18 μg/mL for BjSEv and caused different profiles of cell death, with BjSv being more necrotic. In conclusion, we suggest that BjSv is more nephrotoxic than BjSEv.
authors.affiliation: Centro Acadêmico de Vitória, Universidade Federal de Pernambuco, Vitória de Santo Antão, Pernambuco, Brazil. Electronic address: robertajeanebj@gmail.com.
authors.forename: Roberta Jeane Bezerra
authors.initials: RJ
authors.lastname: Jorge
authors.affiliation: Departamento de Fisiologia e Farmacologia, Universidade Federal do Ceará, Fortaleza, Ceara, Brazil.
authors.forename: Antônio Rafael Coelho
authors.initials: AR
authors.lastname: Jorge
authors.affiliation: Departamento de Fisiologia e Farmacologia, Universidade Federal do Ceará, Fortaleza, Ceara, Brazil.
authors.forename: Ramon Róseo Paula Pessoa Bezerra
authors.initials: RR
authors.lastname: de Menezes
authors.affiliation: Departmento de Análises Clínicas e Toxicológicas, Universidade Federal do Ceará, Fortaleza, Ceara, Brazil.
authors.forename: Clarissa Perdigão
authors.initials: CP
authors.lastname: Mello
authors.affiliation: Departmento de Análises Clínicas e Toxicológicas, Universidade Federal do Ceará, Fortaleza, Ceara, Brazil.
authors.forename: Danya Bandeira
authors.initials: DB
authors.lastname: Lima
authors.affiliation: Departamento de Fisiologia e Farmacologia, Universidade Federal do Ceará, Fortaleza, Ceara, Brazil.
authors.forename: João Alison de Moraes
authors.initials: JA
authors.lastname: Silveira
authors.affiliation: Departamento de Fisiologia e Farmacologia, Universidade Federal do Ceará, Fortaleza, Ceara, Brazil.
authors.forename: Natacha Teresa Queiroz
authors.initials: NT
authors.lastname: Alves
authors.affiliation: Departamento de Fisiologia e Farmacologia, Universidade Federal do Ceará, Fortaleza, Ceara, Brazil.
authors.forename: Aline Diogo
authors.initials: AD
authors.lastname: Marinho
authors.affiliation: Departmento de Antibióticos, Universidade Federal de Pernambuco, Recife, Pernambuco, Brazil.
authors.forename: Rafael Matos
authors.initials: RM
authors.lastname: Ximenes
authors.affiliation: Instituto Nacional de Biologia Estrutural e Bioimagem, Instituto de Bioquímica Médica Leopoldo de Meis - Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil.
authors.forename: Carlos
authors.initials: C
authors.lastname: Corrêa-Netto
authors.affiliation: Instituto Nacional de Biologia Estrutural e Bioimagem, Instituto de Bioquímica Médica Leopoldo de Meis - Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil.
authors.forename: Larissa
authors.initials: L
authors.lastname: Gonçalves Machado
authors.affiliation: Instituto Nacional de Biologia Estrutural e Bioimagem, Instituto de Bioquímica Médica Leopoldo de Meis - Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil.
authors.forename: Russolina Benedeta
authors.initials: RB
authors.lastname: Zingali
authors.affiliation: Departmento de Análises Clínicas e Toxicológicas, Universidade Federal do Ceará, Fortaleza, Ceara, Brazil.
authors.forename: Alice Maria Costa
authors.initials: AM
authors.lastname: Martins
authors.affiliation: Departamento de Fisiologia e Farmacologia, Universidade Federal do Ceará, Fortaleza, Ceara, Brazil.
authors.forename: Helena Serra Azul
authors.initials: HS
authors.lastname: Monteiro
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D017837:Bothrops; D003435:Crotalid Venoms / Q000737:chemistry / Q000633:toxicity*; D004285:Dogs; D066298:In Vitro Techniques; D007668:Kidney / Q000187:drug effects* / Q000473:pathology; D061985:Madin Darby Canine Kidney Cells; D008297:Male; D017208:Rats, Wistar
chemical_list: D003435:Crotalid Venoms
keywords: Bothrops jararaca venom; Isolated kidney perfusion; MDCK; Renal effects