title: [Dihydrotestosterone inhibits foam cell formation via a lectin-like ox-low-density lipoprotein receptor mediated mechanism in J774.1 cell line].
abstract: Objective:To investigate the effect of dihydrotestosterone (DHT) on lectin-like ox- low-density lipoprotein (LDL) receptor(LOX-1)expression and foam cell formation in the female macrophage cell line J774.1.Methods:In cultured J774.1 cells, after pretreated with DHT at concentrations of 1×10-9mol/L and 1×10-8mol/L, ox-LDL-induced LOX-1 expression and foam cell formation were investigated by quantitative real-time PCR, Western blotting, and oil-red O staining.Results:DHT at concentrations of 1×10-9mol/L and 1×10-8mol/L inhibited ox-LDL-induced LOX-1 mRNA (2.81±0.46 and 2.29±0.21 vs 4.71±0.31, bothP<0.01) and protein expression (1.35±0.06 and 1.09±0.04 vs 1.75±0.11, bothP<0.05). The effect was partly reversed by the androgen receptor (AR) blocker flutamide (87.6%,P=0.004). Oil-red O staining also revealed that DHT at concentrations of 1×10-9mol/L and 1×10-8mol/L suppressed ox-LDL-induced foam cell formation as quantified by the number of foam cells per high-power field (HPF) (36.0±3.0 and 29.1±1.3 vs 45.9±3.7, bothP<0.05) and by the area of oil-red O stained particles per HPF (7 983±1 035 and 4 060±390 vs 14 750±2 489, bothP<0.05).Conclusion:DHT at concentrations of 1×10-9mol/L and 1×10-8mol/L decreases LOX-1 expression and foam cell formation via AR.
authors.affiliation: Department of Endocrinology, Shengjing Hospital of China Medical University, Shenyang 110004, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Qiu
authors.forename: H D
authors.initials: HD
authors.lastname: Hu
authors.forename: B Q
authors.initials: BQ
authors.lastname: Hu
authors.forename: X Y
authors.initials: XY
authors.lastname: Chen
authors.forename: P Y
authors.initials: PY
authors.lastname: Xu
authors.forename: L
authors.initials: L
authors.lastname: Cui
authors.forename: P
authors.initials: P
authors.lastname: Li
authors.forename: C
authors.initials: C
authors.lastname: Liu
authors.forename: L
authors.initials: L
authors.lastname: Li
mesh_terms: D002460:Cell Line; D013196:Dihydrotestosterone; D005487:Foam Cells; D006801:Humans; D037102:Lectins; D008077:Lipoproteins, LDL; D008264:Macrophages; D011973:Receptors, LDL
chemical_list: C573412:LDLR protein, human; D037102:Lectins; D008077:Lipoproteins, LDL; D011973:Receptors, LDL; C099252:oxidized low density lipoprotein; D013196:Dihydrotestosterone