title: Encapsulation of amine dehydrogenase in hybrid titania nanoparticles by polyethylenimine coating and templated biomineralization.
abstract: Asymmetric synthesis of chiral amines by amine dehydrogenases (AmDH), which catalyzed reductive amination of ketones with high enantioselectivity, is an ideal route for production of chiral amine. In this study, a facile approach is proposed to immobilize unstable amine dehydrogenase by two steps. Firstly, polyethylenimine (PEI), a cationic polymer, was applied for coating enzyme to inhibit the dissociation of multimeric enzymes. Strong interaction of PEI with AmDH were analyzed and indicated PEI provided the hydrophilic microenvironment for enzyme. The half-life was improved about 18 fold in 50°C. Secondly, to further stabilize AmDH-PEI, PEI coated on AmDH surface was used as a biological template inducing the hydrolysis and condensation of titanium precursor to form titania. AmDH-PEI-Ti possessed more than 80% of the catalytic activity of free enzyme and the entrapment efficiency can be high up to 90%. A mechanistic illustration of the formation of AmDH-PEI-Ti nanoparticles were proposed. Titania provided a rigid cage pocket for the protection from structure unfolding. This generally applicable strategy offers a potential technique for multimeric enzyme immobilization with the advantages of low cost, easy operation, high reservation of activity and high stability.
authors.affiliation: Department of Chemical and Biochemical Engineering, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen, 361005, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Ren
authors.affiliation: Department of Chemical and Biochemical Engineering, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen, 361005, China.
authors.forename: Yonghui
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Chemical and Biochemical Engineering, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen, 361005, China.
authors.forename: Jieru
authors.initials: J
authors.lastname: Su
authors.affiliation: Department of Chemical and Biochemical Engineering, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen, 361005, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Lin
authors.affiliation: Department of Chemical and Biochemical Engineering, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen, 361005, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Wang
authors.affiliation: Department of Chemical and Biochemical Engineering, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen, 361005, China; The Key Lab for Synthetic Biotechnology of Xiamen City, Xiamen University, Xiamen, Fujian, 361005, PR China. Electronic address: fbs@xmu.edu.cn.
authors.forename: Baishan
authors.initials: B
authors.lastname: Fang
authors.affiliation: Department of Chemical and Biochemical Engineering, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen, 361005, China; The Key Lab for Synthetic Biotechnology of Xiamen City, Xiamen University, Xiamen, Fujian, 361005, PR China. Electronic address: szwang@xmu.edu.cn.
authors.forename: Shizhen
authors.initials: S
authors.lastname: Wang
mesh_terms: D001407:Bacillus / Q000201:enzymology / Q000235:genetics; D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D004795:Enzyme Stability; D004926:Escherichia coli / Q000235:genetics; D053768:Metal Nanoparticles / Q000737:chemistry*; D010088:Oxidoreductases / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D011094:Polyethyleneimine / Q000737:chemistry*; D011994:Recombinant Proteins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D014025:Titanium / Q000737:chemistry*
chemical_list: D001426:Bacterial Proteins; D011994:Recombinant Proteins; C009495:titanium dioxide; D011094:Polyethyleneimine; D014025:Titanium; D010088:Oxidoreductases
keywords: Amine dehydrogenase; Bioinspired mineralization; Hybrid immobilization; Multimeric enzyme; Polyethylenimine; Thermostability