title: Transgenic Zebrafish.
abstract: The generation and use of transgenic animals carrying exogenous pieces of DNA stably integrated in their genome is a quite common practice in modern laboratories. Zebrafish have been increasingly used for transgenesis studies mainly due to easy egg accessibility and manipulation together with relatively short generation time. The zebrafish transgenic technology becomes very useful when coupled to continuous in vivo observation of the vertebrate embryonic vasculature. Here we describe the most common technique to generate zebrafish transgenic fish using the Tol2-based methodology and their applications to visualize vascular tissues or endothelial cells in vivo and for functional tumor angiogenesis studies.
authors.affiliation: Vesalius Research Center, VIB-KUL, O&N 9, 9e verd, Campus Gasthuisberg, Herestraat 49, bus 912, 3000, Leuven, Belgium.
authors.forename: Xiaowen
authors.initials: X
authors.lastname: Chen
authors.affiliation: Vesalius Research Center, VIB-KUL, Leuven, Belgium.
authors.forename: Dafne
authors.initials: D
authors.lastname: Gays
authors.affiliation: Vesalius Research Center, VIB-KUL, O&N 9, 9e verd, Campus Gasthuisberg, Herestraat 49, bus 912, 3000, Leuven, Belgium. massimo.santoro@vib.kuleuven.be.|Department of Molecular Biotechnology and Health Sciences, Molecular Biotechnology Center, University of Torino, Torino, Italy. massimo.santoro@vib.kuleuven.be.
authors.forename: Massimo M
authors.initials: MM
authors.lastname: Santoro
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D004251:DNA Transposable Elements; D018014:Gene Transfer Techniques; D016254:Mutagenesis, Insertional; D011401:Promoter Regions, Genetic; D050436:Regulatory Elements, Transcriptional; D015027:Zebrafish / Q000196:embryology / Q000235:genetics*
chemical_list: D004251:DNA Transposable Elements
keywords: Gateway cloning system; Tol2 system; Transgenesis; Zebrafish embryos