title: The phosphorylation of Metaxin 1 controls Bak activation during TNFα induced cell death.
abstract: The proapoptotic protein Bak is implicated in the execution phase of apoptosis, a cell death program. Bak is essentially mitochondrial and during early steps of apoptosis undergoes conformational changes that lead to its full membrane integration in mitochondria and the subsequent liberation of pro-apoptotic mitochondrial proteins. Little is known about the partners and mechanisms implicated in the activation of Bak. We have recently shown that Bak is incorporated into a Voltage dependent anionic channel of type 2 (VDAC2)/Metaxin 1(Mtx1)/Metaxin 2 (Mtx2) multi-protein complex in both resting and dying cells. Here, we show that, after the induction of apoptosis, Bak switches from its association with Mtx2 and VDAC2 to a closer association with Mtx1. This change of partners is under the control of a tyrosine phosphorylation of Mtx1 by c-Abl.
authors.affiliation: Team 9 Centre de Recherche en Cancérologie Nantes-Angers, UMR INSERM 892/CNRS UMR 6299, F-44007 Nantes, France; Université de Nantes, Faculté de Médecine, 9 Quai Moncousu, 44035 Nantes Cedex 01, France.
authors.forename: Elise
authors.initials: E
authors.lastname: Petit
authors.affiliation: Team 9 Centre de Recherche en Cancérologie Nantes-Angers, UMR INSERM 892/CNRS UMR 6299, F-44007 Nantes, France; Université de Nantes, Faculté de Médecine, 9 Quai Moncousu, 44035 Nantes Cedex 01, France; LaBCT, Institut de Cancérologie de l'Ouest-avenue Jean Monnet St Herblain, France.
authors.forename: Pierre-François
authors.initials: PF
authors.lastname: Cartron
authors.affiliation: Team 9 Centre de Recherche en Cancérologie Nantes-Angers, UMR INSERM 892/CNRS UMR 6299, F-44007 Nantes, France; Université de Nantes, Faculté de Médecine, 9 Quai Moncousu, 44035 Nantes Cedex 01, France; CHU de Nantes, 1 place Alexis-Ricordeau, 44093 Nantes Cedex 1, France.
authors.forename: Lisa
authors.initials: L
authors.lastname: Oliver
authors.affiliation: Team 9 Centre de Recherche en Cancérologie Nantes-Angers, UMR INSERM 892/CNRS UMR 6299, F-44007 Nantes, France; Université de Nantes, Faculté de Médecine, 9 Quai Moncousu, 44035 Nantes Cedex 01, France; LaBCT, Institut de Cancérologie de l'Ouest-avenue Jean Monnet St Herblain, France. Electronic address: francois.vallette@inserm.fr.
authors.forename: François M
authors.initials: FM
authors.lastname: Vallette
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D001932:Brain Neoplasms / Q000378:metabolism / Q000473:pathology; D045744:Cell Line, Tumor; D005910:Glioma / Q000378:metabolism / Q000473:pathology; D006801:Humans; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism; D033681:Mitochondrial Membrane Transport Proteins; D008954:Models, Biological; D009363:Neoplasm Proteins / Q000378:metabolism; D010766:Phosphorylation / Q000187:drug effects; D011485:Protein Binding / Q000187:drug effects; D011506:Proteins / Q000378:metabolism*; D053221:TNF-Related Apoptosis-Inducing Ligand / Q000494:pharmacology; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology*; D050998:bcl-2 Homologous Antagonist-Killer Protein / Q000378:metabolism*; D051028:bcl-2-Associated X Protein / Q000172:deficiency / Q000378:metabolism
chemical_list: C093550:MTX1 protein, human; D033681:Mitochondrial Membrane Transport Proteins; D009363:Neoplasm Proteins; D011506:Proteins; D053221:TNF-Related Apoptosis-Inducing Ligand; D014409:Tumor Necrosis Factor-alpha; D050998:bcl-2 Homologous Antagonist-Killer Protein; D051028:bcl-2-Associated X Protein
keywords: Apoptosis; Bak; Glioma; Metaxins; Mitochondria; Protein import