title: Identification of fraud (with pig stuffs) in chicken-processed meat through information of mitochondrial cytochrome b.
abstract: This study was conducted to find out the fraud in chicken-processed meat ingredients to protect consumers from commercial adulteration and authentication through a reliable way: direct amplification of conserved segment of cytochrome b gene of mitochondrial DNA, in addition, using species-specific primer assay for a certain cytochrome b. The results reported that chicken-processed meats were identified as a chicken meat based on amplification of conserved cytochrome b gene of mtDNA, while different fragments sizes were produced after the application of species-specific primer as follows: 227, 157, 274, 331, 389 and 439 bp for raw meat of chicken, goat, cattle, sheep, pig and horse, respectively. The results revealed that all chicken meat products are produced with 227 bp in size. While, an adulteration with pork stuffs was observed in some of the chicken meat products using a species-specific primer of cytochrome b gene, namely, chicken luncheon and chicken burger. This study represents a reliable technique that could be used to provide a promising solution for identifying the commercial adulteration and substitutions in processed meat in retail markets.
authors.affiliation: a Biological Sciences Department, Faculty of Sciences , King Abdul Aziz University , Jeddah , Kingdom of Saudi Arabia.|b Cell Biology Department, Genetic Engineering and Biotechnology Division , National Research Centre , Gizza , Egypt.
authors.forename: Haitham A
authors.initials: HA
authors.lastname: Yacoub
authors.affiliation: a Biological Sciences Department, Faculty of Sciences , King Abdul Aziz University , Jeddah , Kingdom of Saudi Arabia.|c EgyBlood, Vacsera , Cairo , Egypt.
authors.forename: Mahmoud A
authors.initials: MA
authors.lastname: Sadek
mesh_terms: D000818:Animals; D002645:Chickens / Q000235:genetics* / Q000378:metabolism; D045303:Cytochromes b / Q000235:genetics; D005506:Food Contamination / Q000032:analysis*; D050259:Genes, Mitochondrial; D008460:Meat / Q000032:analysis*; D058889:Molecular Typing / Q000379:methods*; D016133:Polymerase Chain Reaction / Q000379:methods; D013552:Swine / Q000235:genetics* / Q000378:metabolism
chemical_list: D045303:Cytochromes b
keywords: Adulteration; chicken; cytochrome b gene; meat; mtDNA