title: Label-Free 3D Ag Nanoflower-Based Electrochemical Immunosensor for the Detection of Escherichia coli O157:H7 Pathogens.
abstract: It is highly desirable to develop a rapid and simple method to detect pathogens. Combining nanomaterials with electrochemical techniques is an efficient way for pathogen detection. Herein, a novel 3D Ag nanoflower was prepared via a biomineralization method by using bovine serum albumin (BSA) as a template. It was adopted as a sensing interface to construct an electrochemical bacteria immunosensor for the rapid detection of foodborne pathogens Escherichia coli (E. coli) O157:H7. Bacterial antibody was immobilized onto the surface of Ag nanoflowers through covalent conjugation. Electrochemical impedance spectroscopy (EIS) was used to detect and validate the resistance changes, where [Fe(CN)6]3-/4-acted as the redox probe. A linear relation between Retand E. coli concentration was obtained in the E. coli concentration range of 3.0 × 102-3.0 × 108cfu mL-1. The as-prepared biosensor gave rise to an obvious response to E. coli but had no distinct response to Cronobacter sakazakii, methicillin-resistant Staphylococcus aureus (MRSA), Staphylococcus albus, Lactobacillus easei, and Shigella flexneri, revealing a high selectivity for the detection of the pathogens down to 100 cfu mL-1in a short time. We believe that this BSA-conjugated 3D Ag nanoflowers could be used as a powerful interface material with good conductivity and biocompatibility for improving pathogen detection and treatment in the field of medicine, environment, and food safety.
authors.affiliation: Department of Plastic and Reconstructive Surgery, Shanghai Key Laboratory of Tissue Engineering, National Tissue Engineering Center of China, Shanghai 9th People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, People's Republic of China.
authors.forename: He
authors.initials: H
authors.lastname: Huang
authors.affiliation: College of Chemical Engineering & Materials Science, Quanzhou Normal University, Quanzhou, China.
authors.forename: Minghuan
authors.initials: M
authors.lastname: Liu
authors.affiliation: Department of Plastic and Reconstructive Surgery, Shanghai Key Laboratory of Tissue Engineering, National Tissue Engineering Center of China, Shanghai 9th People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, People's Republic of China.
authors.forename: Xiangsheng
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Plastic and Reconstructive Surgery, Shanghai Key Laboratory of Tissue Engineering, National Tissue Engineering Center of China, Shanghai 9th People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, People's Republic of China.
authors.forename: Wenjie
authors.initials: W
authors.lastname: Zhang
authors.affiliation: College of Chemical Engineering & Materials Science, Quanzhou Normal University, Quanzhou, China. yangdp@qztc.edu.cn.
authors.forename: Da-Peng
authors.initials: DP
authors.lastname: Yang
authors.affiliation: School of Medicine, Qingdao University, Qingdao, China.
authors.forename: Lianhua
authors.initials: L
authors.lastname: Cui
authors.affiliation: Department of Plastic and Reconstructive Surgery, Shanghai Key Laboratory of Tissue Engineering, National Tissue Engineering Center of China, Shanghai 9th People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, People's Republic of China. wonderluis@126.com.
authors.forename: Xiansong
authors.identifier: http://orcid.org/0000-0001-5836-6601
authors.initials: X
authors.lastname: Wang
keywords: 3D Ag nanoflowers; Bovine serum albumin; E. coli; Electrochemical immunosensor