title: Integrative analysis for the role of long non-coding RNAs in radiation-induced mouse thymocytes responses.
abstract: Long non-coding RNAs (lncRNAs) are a critical class of regulatory molecules involved in a variety of biological functions; however, their role in immune cells response to radiation is unknown. Therefore, in this study we used integrative analysis to determine the expression profile of lncRNAs in mouse thymocytes and the potential functions of lncRNAs in response to radiation. Microarray data profiling indicated that 53 lncRNAs (36 up-regulated and 17 down-regulated) and 74 coding genes (39 up-regulated and 35 down-regulated) were highly differentially expressed in the high dose radiation (HDR) group compared with the control group. In the low dose radiation (LDR) group, only one lncRNA was down-regulated. Moreover, as compared with the control group, 109 lncRNA pathways in the HDR group and 14 lncRNA pathways in the LDR group were differentially expressed. Our data revealed the expression pattern of lncRNAs in mouse thymocytes and predicted their potential functions in response to LDR and HDR. In the HDR group, GO analysis showed that the role of lncRNAs in damage responses of thymocytes to HDR mainly involved chromatin organization and cell death. These findings might improve our understanding of the role of lncRNAs in LDR- and HDR-induced immune cells and provide a new experimental basis for further investigation.
authors.affiliation: Ministry of Health Key Laboratory of Radiobiology, Jilin University, Changchun 130021, China.|Department of Orthopedics, The First Hospital of Jilin University, Changchun 130021, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Gao
authors.affiliation: Ministry of Health Key Laboratory of Radiobiology, Jilin University, Changchun 130021, China.
authors.forename: Zhuo
authors.initials: Z
authors.lastname: Dong
authors.affiliation: Ministry of Health Key Laboratory of Radiobiology, Jilin University, Changchun 130021, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Wei
authors.affiliation: Ministry of Health Key Laboratory of Radiobiology, Jilin University, Changchun 130021, China.
authors.forename: Lihong
authors.initials: L
authors.lastname: Shao
authors.affiliation: Ministry of Health Key Laboratory of Radiobiology, Jilin University, Changchun 130021, China.
authors.forename: Linlin
authors.initials: L
authors.lastname: Jin
authors.affiliation: Ministry of Health Key Laboratory of Radiobiology, Jilin University, Changchun 130021, China.
authors.forename: Yahui
authors.initials: Y
authors.lastname: Lv
authors.affiliation: Ministry of Health Key Laboratory of Radiobiology, Jilin University, Changchun 130021, China.
authors.forename: Gang
authors.initials: G
authors.lastname: Zhao
authors.affiliation: Ministry of Health Key Laboratory of Radiobiology, Jilin University, Changchun 130021, China jinsz@jlu.edu.cn.
authors.forename: Shunzi
authors.initials: S
authors.lastname: Jin
mesh_terms: D000818:Animals; D015536:Down-Regulation; D051379:Mice; D008813:Mice, Inbred ICR; D062085:RNA, Long Noncoding / Q000502:physiology*; D060168:Thymocytes / Q000166:cytology / Q000528:radiation effects*; D015854:Up-Regulation
chemical_list: D062085:RNA, Long Noncoding
keywords: long non-coding RNAs; mouse thymocytes; radiation effects; response mechanism