title: Association betweenGSTM1andGSTT1polymorphisms and susceptibility to methamphetamine dependence.
abstract: Glutathione S-transferases (GSTs; EC: 2.5.1.18) are ubiquitous multifunctional enzymes, which play a key role in cellular detoxification. Functional genetic polymorphisms in genes encodingGSTM1(a member of GST class mu; OMIM: 138350), andGSTT1(a member of GST class theta; OMIM: 600436) have been well defined. The functional null alleles of GSTM1 andGSTT1represent deletions ofGSTM1andGSTT1genes, respectively. The aim of the present study is to investigate the association betweenGSTM1andGSTT1polymorphisms and methamphetamine dependence. The present population-based case-control study was performed in Shiraz (southern Iran). In total, 52 methamphetamine dependence (11 females, 41 males) and 635 healthy controls (110 females, 525 males) were included in this study. The genotypes ofGSTM1andGSTT1polymorphisms were determined by PCR. NeitherGSTM1(OR=0.92, 95% CI: 0.52-1.61, P=0.771) norGSTT1(OR=0.71, 95% CI: 0.33-1.54, P=0.381) null genotypes were significantly associated with risk of methamphetamine dependence. It should be noted that although there was no association between theGSTM1null genotype and risk of methamphetamine dependence, in both genders, there was significant interaction between gender andGSTM1polymorphism (P=0.029). The combination genotypes of theGSTM1andGSTT1polymorphisms revealed that the genotypes of these two polymorphisms had no additive effect in relation to the susceptibility to methamphetamine dependence. The present study revealed that genetic polymorphisms ofGSTT1andGSTM1are not risk factors for methamphetamine dependence.
authors.affiliation: Department of Biology, College of Sciences, Shiraz University, Shiraz 71454, Iran.
authors.forename: Mohammad Rashid
authors.initials: MR
authors.lastname: Khalighinasab
authors.affiliation: Department of Biology, College of Sciences, Shiraz University, Shiraz 71454, Iran.
authors.forename: Khyber
authors.initials: K
authors.lastname: Saify
authors.affiliation: Department of Biology, College of Sciences, Shiraz University, Shiraz 71454, Iran.
authors.forename: Mostafa
authors.initials: M
authors.lastname: Saadat
keywords: GSTM1; GSTT1; Methamphetamine dependence; Polymorphism