title: Creating biomolecular motors based on dynein and actin-binding proteins.
abstract: Biomolecular motors such as myosin, kinesin and dynein are protein machines that can drive directional movement along cytoskeletal tracks and have the potential to be used as molecule-sized actuators. Although control of the velocity and directionality of biomolecular motors has been achieved, the design and construction of novel biomolecular motors remains a challenge. Here we show that naturally occurring protein building blocks from different cytoskeletal systems can be combined to create a new series of biomolecular motors. We show that the hybrid motors-combinations of a motor core derived from the microtubule-based dynein motor and non-motor actin-binding proteins-robustly drive the sliding movement of an actin filament. Furthermore, the direction of actin movement can be reversed by simply changing the geometric arrangement of these building blocks. Our synthetic strategy provides an approach to fabricating biomolecular machines that work along artificial tracks at nanoscale dimensions.
authors.affiliation: Advanced ICT Research Institute, National Institute of Information and Communications Technology, Kobe, Hyogo 651-2492, Japan.
authors.forename: Akane
authors.initials: A
authors.lastname: Furuta
authors.affiliation: Advanced ICT Research Institute, National Institute of Information and Communications Technology, Kobe, Hyogo 651-2492, Japan.
authors.forename: Misako
authors.initials: M
authors.lastname: Amino
authors.affiliation: Advanced ICT Research Institute, National Institute of Information and Communications Technology, Kobe, Hyogo 651-2492, Japan.
authors.forename: Maki
authors.initials: M
authors.lastname: Yoshio
authors.affiliation: Advanced ICT Research Institute, National Institute of Information and Communications Technology, Kobe, Hyogo 651-2492, Japan.|CREST, Japan Science and Technology Agency, Chiyoda-ku, Tokyo 102-0076, Japan.|Graduate School of Life Science, University of Hyogo, Harima Science Park City, Hyogo 678-1297, Japan.
authors.forename: Kazuhiro
authors.identifier: 0000-0002-1281-113X
authors.initials: K
authors.lastname: Oiwa
authors.affiliation: Advanced ICT Research Institute, National Institute of Information and Communications Technology, Kobe, Hyogo 651-2492, Japan.
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Kojima
authors.affiliation: Advanced ICT Research Institute, National Institute of Information and Communications Technology, Kobe, Hyogo 651-2492, Japan.
authors.forename: Ken'ya
authors.initials: K
authors.lastname: Furuta
mesh_terms: D003599:Cytoskeleton / Q000737:chemistry* / Q000235:genetics; D004398:Dyneins / Q000737:chemistry* / Q000235:genetics; D006801:Humans; D008840:Microfilament Proteins / Q000737:chemistry* / Q000235:genetics
chemical_list: D008840:Microfilament Proteins; D004398:Dyneins