title: Intramyocardial Injection of siRNAs Can Efficiently Establish Myocardial Tissue-Specific Renalase Knockdown Mouse Model.
abstract: Ischaemia/reperfusion (I/R) injury will cause additional death of cardiomyocytes in ischaemic heart disease. Recent studies revealed that renalase was involved in the I/R injury. So, the myocardial tissue-specific knockdown mouse models were needed for the investigations of renalase. To establish the mouse models, intramyocardial injection of siRNAs targeting renalase was performed in mice. The wild distribution and high transfection efficiency of the siRNAs were approved. And the renalase expression was efficiently suppressed in myocardial tissue. Compared with the high cost, time consumption, and genetic compensation risk of the Cre/loxP technology, RNA interference (RNAi) technology is much cheaper and less time-consuming. Among the RNAi technologies, injection of siRNAs is safer than virus. And considering the properties of the I/R injury mouse models, the efficiency and durability of injection with siRNAs are acceptable for the studies. Altogether, intramyocardial injection of siRNAs targeting renalase is an economical, safe, and efficient method to establish myocardial tissue-specific renalase knockdown mouse models.
authors.affiliation: Institution of Cardiology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, China.
authors.forename: Kun
authors.identifier: 0000-0001-7378-5660
authors.initials: K
authors.lastname: Huang
authors.affiliation: Department of Geriatrics, Affiliated Hospital of Wuhan Traditional Chinese Medicine and West Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, China.
authors.forename: Ju
authors.initials: J
authors.lastname: Liu
authors.affiliation: Department of Art, International Education College, Wuhan University of Technology, Wuhan, Hubei, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Department of Gastrointestinal Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, China.
authors.forename: Jiliang
authors.identifier: 0000-0001-6169-637X
authors.initials: J
authors.lastname: Wang
authors.affiliation: Department of Gastrointestinal Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, China.
authors.forename: Huili
authors.identifier: 0000-0001-7508-1122
authors.initials: H
authors.lastname: Li
mesh_terms: D000818:Animals; D055785:Gene Knockdown Techniques / Q000379:methods*; D019426:Integrases; D008297:Male; D051379:Mice; D018345:Mice, Knockout; D008995:Monoamine Oxidase; D015428:Myocardial Reperfusion Injury / Q000235:genetics / Q000628:therapy; D009206:Myocardium / Q000378:metabolism* / Q000473:pathology; D009928:Organ Specificity; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics* / Q000494:pharmacology*
chemical_list: D034741:RNA, Small Interfering; D008995:Monoamine Oxidase; C521557:renalase; C045073:Cre recombinase; D019426:Integrases