title: Gadolinium containing telechelic PEG-polymers end-capped by di-phenylalanine motives as potential supramolecular MRI contrast agents.
abstract: Telechelic PEG-polymers end-capped by diphenylalanine (FF) motives and containing a DOTA-Gd complex, bound on a lysine side chain at the centre of peptide moiety, are studied for their assembling properties and for the relaxometric behavior. The observed variations in terms of relaxivity are correlated to the assembling properties of the aggregates by using several techniques: fluorescence, Circular Dichroism (CD) and Fourier Transform Infrared (FTIR) for aggregation tendency and secondary structure determination; Dynamic Light Scattering (DLS) and Transmission Electron Microscopy (TEM) for morphological definition. Self-aggregation in water solution of the peptide conjugates, due to interaction of the phenylalanine frameworks, starts at concentration around 1 mg/ml with a first evidence of the coexistence of fibrillary networks and hydrogels at 10 mg/ml. Definitive presence of well-structured fibrillary networks, dominated by an antiparallel β-sheet arrangement, occurs at 50 mg/ml. At the latter concentration relaxivity values measured at 20 MHz and 298 K, are around 11 mM-1s-1, in line with a possible use of the these aggregates as MRI contrast agents. Copyright © 2016 European Peptide Society and John Wiley & Sons, Ltd.
authors.affiliation: Department of Pharmacy, Research Centre on Bioactive Peptides (CIRPeB), University of Naples "Federico II", Via Mezzocannone 16, 80134, Naples, Italy.
authors.forename: Carlo
authors.initials: C
authors.lastname: Diaferia
authors.affiliation: Department of Molecular Biotechnologies and Health Science, University of Turin, Via Nizza 52, 10125, Turin, Italy.
authors.forename: Eliana
authors.initials: E
authors.lastname: Gianolio
authors.affiliation: Department of Pharmacy, Research Centre on Bioactive Peptides (CIRPeB), University of Naples "Federico II", Via Mezzocannone 16, 80134, Naples, Italy.
authors.forename: Antonella
authors.initials: A
authors.lastname: Accardo
authors.affiliation: Department of Pharmacy, Research Centre on Bioactive Peptides (CIRPeB), University of Naples "Federico II", Via Mezzocannone 16, 80134, Naples, Italy.
authors.forename: Giancarlo
authors.initials: G
authors.lastname: Morelli
mesh_terms: D003287:Contrast Media / Q000138:chemical synthesis / Q000737:chemistry*; D004151:Dipeptides; D005682:Gadolinium / Q000737:chemistry*; D020100:Hydrogels / Q000737:chemistry; D057927:Hydrophobic and Hydrophilic Interactions; D008279:Magnetic Resonance Imaging / Q000295:instrumentation; D010455:Peptides / Q000138:chemical synthesis / Q000737:chemistry*; D010649:Phenylalanine / Q000031:analogs & derivatives; D011092:Polyethylene Glycols / Q000737:chemistry*; D017433:Protein Structure, Secondary; D014867:Water / Q000737:chemistry
chemical_list: D003287:Contrast Media; D004151:Dipeptides; D020100:Hydrogels; D010455:Peptides; D014867:Water; C026650:phenylalanylphenylalanine; D011092:Polyethylene Glycols; D010649:Phenylalanine; D005682:Gadolinium
keywords: MRI contrast agents; nanofibers; oligo-phenylalanines; self-assembling; telechelic polimers