title: Association of locally produced IL10 and TGFb1 with tumor size, histological type and presence of metastases in patients with lung carcinoma.
abstract: OBJECTIVE
  Advanced lung carcinoma is charasterized with fast disease progression. Interleukin (IL)10 and transforming growth factor (TGF)b1 are immunosuppressive mediators and their role in lung carcinoma pathogenesis and in the antitumor response has not yet been elucidated. The purpose of this study was to correlate IL10 and TGFb1 levels in the serum and lung tumor microcirculation with clinical stage, disease extent, histological features and TNM stage.
  
  
  METHODS
  The study included 41 lung cancer patients in clinical stage III and IV. Histological type was determined immunohistochemically, while tumor size, localization and dissemination were determined radiologically by multislice computerized tomography (MSCT). IL10 and TGFb1 levels were quantified with commercial flow cytometric test in serum and lung tumor microcirculation samples.
  
  
  RESULTS
  Non small cell lung cancer (NSCLC) patients had significantly elevated TGFb1 while small cell lung cancer (SCLC) patients had significantly increased IL10 in tumor microcirculation. IL10 was significantly elevated in patients with the largest tumors, as well as in patients with III clinical stage and without metastases, both in the serum and tumor microcirculation. TGFb1 was significantly increased in serum and tumor microcirculation in patients with larger tumors. We found significant correlation between these two immunosuppressive cytokines, IL10 and TGFb1, in tumor microcirculation but not in patient serum samples.
  
  
  CONCLUSIONS
  IL10 and TGFb1 in systemic and tumor microcirculation are significantly associated with particular histological type of lung cancer, tumor size and degree of disease extent.
authors.affiliation: Clinic for Lung diseases, Military Medical Academy (MMA), Belgrade, Serbia Serbia.
authors.forename: Vukoica
authors.initials: V
authors.lastname: Karlicic
authors.forename: Jelena
authors.initials: J
authors.lastname: Vukovic
authors.forename: Ivan
authors.initials: I
authors.lastname: Stanojevic
authors.forename: Jelena
authors.initials: J
authors.lastname: Sotirovic
authors.forename: Aleksandar
authors.initials: A
authors.lastname: Peric
authors.forename: Milena
authors.initials: M
authors.lastname: Jovic
authors.forename: Vlado
authors.initials: V
authors.lastname: Cvijanovic
authors.forename: Mirjana
authors.initials: M
authors.lastname: Djukic
authors.forename: Tatjana
authors.initials: T
authors.lastname: Banovic
authors.forename: Danilo
authors.initials: D
authors.lastname: Vojvodic
mesh_terms: D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000097:blood*; D002277:Carcinoma / Q000097:blood* / Q000000981:diagnostic imaging / Q000556:secondary*; D016022:Case-Control Studies; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D016753:Interleukin-10 / Q000097:blood*; D008175:Lung Neoplasms / Q000097:blood* / Q000000981:diagnostic imaging / Q000473:pathology*; D008297:Male; D008833:Microcirculation; D008875:Middle Aged; D061330:Multidetector Computed Tomography; D009367:Neoplasm Staging; D011237:Predictive Value of Tests; D011652:Pulmonary Circulation; D053773:Transforming Growth Factor beta1 / Q000097:blood*; D047368:Tumor Burden
chemical_list: D014408:Biomarkers, Tumor; C508609:IL10 protein, human; C508960:TGFB1 protein, human; D053773:Transforming Growth Factor beta1; D016753:Interleukin-10