title: Evaluation and Comparison of the Antibacterial Activity againstStreptococcus mutansof Grape Seed Extract at Different Concentrations with Chlorhexidine Gluconate: Anin vitroStudy.
abstract: BACKGROUND
  Streptococcus mutanshas been implicated as primary microorganisms which cause dental caries in humans. There has been an increased interest in the therapeutic properties of some medicinal plants and natural compounds which have demonstrated antibacterial activities. Grape is one of the plants of this group which contains tannin and polyphenolic compound.
  
  
  OBJECTIVE
  To evaluate and compare antibacterial activity of grape seed extract at different concentrations with chlorhexidine gluconate against S.mutans.
  
  
  METHODS
  Grape seeds were extracted with ethanol/water ratio of 70:30 volume/volume. The extracts were filtered through Whatman No. 1 filter paper until it becomes colorless.Streptococcus mutansstrains were taken. To check the antimicrobial properties of grape seed extract at different concentration and chlorhexidine gluconate, they were added to S.mutansstrain and incubated for 48 hours than colony-forming units/mL were checked.
  
  
  RESULTS
  Grape seed extract at higher concentration were found to be more potent againstS. mutans.Chlorhexidine gluconate was found to have most potent antibacterial action compared to all different concentrations of grape seed extract.
  
  
  CONCLUSIONS
  Grape seed extract as a natural antimicrobial compound has inhibitory effect against S.mutans.
  Swadas M, Dave B, Vyas SM, Shah N. Evaluation and Comparison of the Antibacterial Activity againstStreptococcus mutansof Grape Seed Extract at Different Concentrations with Chlorhexidine Gluconate: Anin vitroStudy. Int J Clin Pediatr Dent 2016;9(3):181-185.
authors.affiliation: Senior Lecturer, Department of Pedodontics and Preventive Dentistry Narsinhbhai Patel Dental College and Hospital, Visnagar Gujarat, India.
authors.forename: Milan
authors.initials: M
authors.lastname: Swadas
authors.affiliation: Professor and Head, Department of Pedodontics and Preventive Dentistry, KM Shah Dental College and Hospital, Sumadeep Vidyapeeth University, Vadodara, Gujarat, India.
authors.forename: Bhavna
authors.initials: B
authors.lastname: Dave
authors.affiliation: Postgraduate Student, Department of Pedodontics and Preventive Dentistry, KM Shah Dental College and Hospital, Sumadeep Vidyapeeth University, Vadodara, Gujarat, India.
authors.forename: Soham M
authors.initials: SM
authors.lastname: Vyas
authors.affiliation: Postgraduate Student, Department of Pedodontics and Preventive Dentistry, KM Shah Dental College and Hospital, Sumadeep Vidyapeeth University, Vadodara, Gujarat, India.
authors.forename: Nupur
authors.initials: N
authors.lastname: Shah
keywords: Antimicrobial; Grape seed extract; Streptococcus mutans.