title: Reduction of GAPDH in lenses of Parkinson's disease patients: A possible new biomarker.
abstract: We previously showed that the protein pattern of lenses removed in cataract surgery differs between patients with Parkinson's disease and age-matched controls. In this study, we identified the protein reduced in abundance in the 34- to 37-kDa gel band.
  
  
  
  During cataract surgery (phacoemulsification), we collected the rinsing fluid and lens particles from the eyes of PD patients and controls. Residual lens fragments in the supernatant of 3 PD patients and aged-matched controls were studied for protein profiles using liquid chromatography-mass spectrometry and Western blots.
  
  
  
  We identified glyceraldehyde-3-phosphate dehydrogenase by mass spectrometry as the protein reduced in abundance and verified this finding in Western blots.
  
  
  
  Glyceraldehyde-3-phosphate dehydrogenase has been implicated in PD development. The reduction of glyceraldehyde-3-phosphate dehydrogenase in the lenses of PD patients may be a new biomarker for PD and might also indicate an important role for this protein in PD development. © 2016 International Parkinson and Movement Disorder Society.
authors.affiliation: Department of Ophthalmology, University Hospital Schleswig-Holstein, Campus Kiel, Kiel, Germany.
authors.forename: Alexa
authors.initials: A
authors.lastname: Klettner
authors.affiliation: Systematic Proteomics & Bioanalytics, Inst. Experimental Medicine, Christian-Albrechts-Universität zu Kiel, Kiel, Germany.
authors.forename: Andreas
authors.initials: A
authors.lastname: Tholey
authors.affiliation: Systematic Proteomics & Bioanalytics, Inst. Experimental Medicine, Christian-Albrechts-Universität zu Kiel, Kiel, Germany.
authors.forename: Alena
authors.initials: A
authors.lastname: Wiegandt
authors.affiliation: Department of Ophthalmology, University Hospital Schleswig-Holstein, Campus Kiel, Kiel, Germany.
authors.forename: Elisabeth
authors.initials: E
authors.lastname: Richert
authors.affiliation: Department of Ophthalmology, University Hospital Schleswig-Holstein, Campus Kiel, Kiel, Germany.
authors.forename: Bernhard
authors.initials: B
authors.lastname: Nölle
authors.affiliation: Department of Neurology, University Hospital Schleswig-Holstein, Campus Kiel, Kiel, Germany.
authors.forename: Günther
authors.initials: G
authors.lastname: Deuschl
authors.affiliation: Department of Ophthalmology, University Hospital Schleswig-Holstein, Campus Kiel, Kiel, Germany.
authors.forename: Johann
authors.initials: J
authors.lastname: Roider
authors.affiliation: Department of Neurology, University Hospital Schleswig-Holstein, Campus Kiel, Kiel, Germany.|Department of Neurology, Ludwig-Maximilians-University Munich, Klinikum Großhadern, Munich, Germany.
authors.forename: Susanne A
authors.initials: SA
authors.lastname: Schneider
mesh_terms: D000368:Aged; D015415:Biomarkers / Q000378:metabolism; D002387:Cataract Extraction; D024581:Glyceraldehyde-3-Phosphate Dehydrogenase (Phosphorylating) / Q000378:metabolism*; D006801:Humans; D007908:Lens, Crystalline / Q000378:metabolism*; D010300:Parkinson Disease / Q000175:diagnosis / Q000378:metabolism*
chemical_list: D015415:Biomarkers; C579041:GAPDHS protein, human; D024581:Glyceraldehyde-3-Phosphate Dehydrogenase (Phosphorylating)
keywords: GAPDH; Parkinson's disease; cataract