title: Genetic and Environmental Biomarkers Associated with Triglyceride Levels in Two Groups of Slovak Women.
abstract: OBJECTIVE
  This study analyzed the association between the MLXIPL gene polymorphism (rs3812316) and triglyceride (TG) levels and selected environmental biomarkers in Slovak women at risk for cardiovascular disease compared to a reference sample.
  
  
  METHODS
  The studied sample consisted of 200 women at cardiovascular risk (mean age 52.96 ± 6.01 years) and 244 healthy women (mean age 47.52 ± 5.34 years). Participants gave details of their health and lifestyle during their medical examination, and peripheral blood samples were used for biochemical analyses and DNA genotyping. A nested polymerase chain reaction-restriction fragment length polymorphism assay was used to detect the rs 3812316 SNP.
  
  
  RESULTS
  We determined that there were significantly different genotype distributions in two TG categories: (1) subjects with normal TG values had a significantly higher G allele frequency than those with elevated TG levels (χ2= 6.1556, df = 2, p = 0.046); and (2) the rare G allele frequency was 0.11 in the cardiovascular risk group and 0.15 in the reference group. Binary regression analysis showed that women with at least one G allele had a significantly lower relative risk of hypertriglyceridemia than women with the CC genotype (OR = 0.399, p = 0.022, 95% CI = 0.182-0.876).
  
  
  CONCLUSIONS
  This cross-sectional study suggests that MLXIPL rs3812316 genotypes may be associated with TG levels. However, further analysis is advisable because of study limitations.
authors.affiliation: 1 Jessenius Faculty of Medicine in Martin (JFM CU), Biomedical Center Martin JFM CU, Comenius University in Bratislava , Martin, Slovakia .
authors.forename: Zuzana
authors.initials: Z
authors.lastname: Danková
authors.affiliation: 2 Department of Anthropology, Faculty of Natural Sciences, Comenius University in Bratislava , Bratislava, Slovakia .
authors.forename: Lenka
authors.initials: L
authors.lastname: Vorobel'ová
authors.affiliation: 2 Department of Anthropology, Faculty of Natural Sciences, Comenius University in Bratislava , Bratislava, Slovakia .
authors.forename: Veronika
authors.initials: V
authors.lastname: Čerňanová
authors.affiliation: 2 Department of Anthropology, Faculty of Natural Sciences, Comenius University in Bratislava , Bratislava, Slovakia .
authors.forename: Darina
authors.initials: D
authors.lastname: Drozdová
authors.affiliation: 1 Jessenius Faculty of Medicine in Martin (JFM CU), Biomedical Center Martin JFM CU, Comenius University in Bratislava , Martin, Slovakia .
authors.forename: Marian
authors.initials: M
authors.lastname: Grendár
authors.affiliation: 3 Department of Molecular Biology, Faculty of Natural Sciences, Comenius University in Bratislava , Bratislava, Slovakia .
authors.forename: Marian
authors.initials: M
authors.lastname: Baldovič
authors.affiliation: 2 Department of Anthropology, Faculty of Natural Sciences, Comenius University in Bratislava , Bratislava, Slovakia .
authors.forename: Marta
authors.initials: M
authors.lastname: Cvíčelová
authors.affiliation: 2 Department of Anthropology, Faculty of Natural Sciences, Comenius University in Bratislava , Bratislava, Slovakia .
authors.forename: Daniela
authors.initials: D
authors.lastname: Siváková
mesh_terms: D000328:Adult; D000483:Alleles; D051778:Basic Helix-Loop-Helix Leucine Zipper Transcription Factors / Q000097:blood / Q000235:genetics*; D015415:Biomarkers / Q000097:blood; D002318:Cardiovascular Diseases / Q000097:blood* / Q000235:genetics*; D016022:Case-Control Studies; D003430:Cross-Sectional Studies; D005260:Female; D005787:Gene Frequency; D056726:Genetic Association Studies; D006801:Humans; D015228:Hypertriglyceridemia / Q000097:blood / Q000235:genetics; D008055:Lipids / Q000097:blood; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D012307:Risk Factors; D018154:Slovakia; D014280:Triglycerides / Q000097:blood* / Q000235:genetics*
chemical_list: D051778:Basic Helix-Loop-Helix Leucine Zipper Transcription Factors; D015415:Biomarkers; D008055:Lipids; C116385:MLXIPL protein, human; D014280:Triglycerides
keywords: MLXIPL; fat mass; physical activity; plasma lipids; smoking