title: Detergent assisted ultrasonication aided in situ transesterification for biodiesel production from oleaginous yeast wet biomass.
abstract: In situ transesterification of oleaginous yeast wet biomass for fatty acid methyl esters (FAMEs) production using acid catalyst, methanol with or without N-Lauroyl sarcosine (N-LS) treatment was performed. The maximum FAMEs yield obtained with or without N-LS treatment in 24h reaction time was 96.1±1.9 and 71±1.4% w/w, respectively. The N-LS treatment of biomass followed by with or without ultrasonication revealed maximum FAMEs yield of 94.3±1.9% and 82.9±1.8% w/w using methanol to lipid molar ratio 360:1 and catalyst concentration 360mM (64μL H2SO4/g lipid) within 5 and 25min reaction time, respectively. The FAMEs composition obtained in in situ transesterification was similar to that obtained with conventional two step lipid extraction and transesterification process. Biodiesel fuel properties (density, kinematic viscosity, cetane number and total glycerol) were in accordance with international standard (ASTM D6751), which suggests the suitability of biodiesel as a fuel.
authors.affiliation: INRS Eau, Terre et Environnement, 490, rue de la Couronne, Québec G1K 9A9, Canada.
authors.forename: Sravan Kumar
authors.initials: SK
authors.lastname: Yellapu
authors.affiliation: INRS Eau, Terre et Environnement, 490, rue de la Couronne, Québec G1K 9A9, Canada.
authors.forename: Rajwinder
authors.initials: R
authors.lastname: Kaur
authors.affiliation: INRS Eau, Terre et Environnement, 490, rue de la Couronne, Québec G1K 9A9, Canada. Electronic address: rd.tyagi@ete.inrs.ca.
authors.forename: Rajeshwar D
authors.initials: RD
authors.lastname: Tyagi
mesh_terms: D056804:Biofuels; D018533:Biomass; D001709:Biotechnology / Q000379:methods*; D003902:Detergents / Q000494:pharmacology*; D004951:Esterification / Q000187:drug effects; D004952:Esters / Q000378:metabolism; D008055:Lipids / Q000302:isolation & purification; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000378:metabolism*; D012521:Sarcosine / Q000494:pharmacology; D013464:Sulfuric Acids / Q000494:pharmacology; D013997:Time Factors; D014465:Ultrasonics / Q000379:methods*; D014867:Water / Q000737:chemistry*
chemical_list: D056804:Biofuels; D003902:Detergents; D004952:Esters; D008055:Lipids; D013464:Sulfuric Acids; D014867:Water; C033158:sulfuric acid; D012521:Sarcosine
keywords: Biodiesel; In situ transesterification; Microbial lipid; N-Lauroyl sarcosine; Ultrasonication