title: In vitro cow uterine response to Escherichia coli, leukotrienes and cytokines.
abstract: The aim was to determine the dynamic profile of interactions between Escherichia coli (E. coli) and the actions of leukotrienes (LTs) and TNF and INFγ (cytokines) in the uterus in vitro. Uterine explants (N=6) were incubated for 2, 12 and 24h either as E. coli-treated (106CFU) or non-treated and/or with: LTB4and C4(10-6M, for both LTs), LTs receptors antagonists (aLTR; 10-6M) and/or cytokines (each 10ng/ml). Toll Like Receptor 4 (TLR4) mRNA expression increased in explants incubated with E. coli, cytokines and LTs after 2 and 12h and aLTR inhibited the effect of LTs in explants incubated with E. coli (P<0.05). IL-6 mRNA expression was up-regulated in E.coli-treated explants with cytokines after 2h and cytokines with LTs after 12h (P<0.05). E. coli increased prostaglandin (PG)E2output after all examined time points, and PGF2αand IL-6 levels in E.coli-treated explants after 12 and 24h with cytokines, with LTs (P<0.05). aLTR inhibited LT stimulating action on PGs and IL-6 output in explants incubated with E. coli after 12 and 24h (P<0.05). LTs modify and enhance experimentally induced infection: TLR4 and IL-6 mRNA expression, IL-6 and PGs secretion, and cytokines participate in this process.
authors.affiliation: Department of Reproductive Immunology and Pathology, Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, 10-747 Olsztyn, Poland. Electronic address: a.korzekwa@pan.olsztyn.pl.
authors.forename: A J
authors.initials: AJ
authors.lastname: Korzekwa
authors.affiliation: Department of Reproductive Immunology and Pathology, Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, 10-747 Olsztyn, Poland.
authors.forename: M
authors.initials: M
authors.lastname: Łupicka
authors.affiliation: Department of Reproductive Immunology and Pathology, Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, 10-747 Olsztyn, Poland.
authors.forename: B M-
authors.initials: BM
authors.lastname: Socha
authors.affiliation: Department of Reproductive Immunology and Pathology, Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, 10-747 Olsztyn, Poland.
authors.forename: A A
authors.initials: AA
authors.lastname: Szczepańska
authors.affiliation: Department of Reproductive Immunology and Pathology, Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, 10-747 Olsztyn, Poland.
authors.forename: E
authors.initials: E
authors.lastname: Piotrowicz
authors.affiliation: Department of Animal Reproduction With Clinic, Faculty of Veterinary Medicine, University of Warmia and Mazury, Olsztyn, Poland.
authors.forename: W
authors.initials: W
authors.lastname: Barański
mesh_terms: D000818:Animals; D002417:Cattle / Q000235:genetics / Q000276:immunology*; D002418:Cattle Diseases / Q000235:genetics / Q000276:immunology; D016207:Cytokines / Q000235:genetics / Q000276:immunology* / Q000494:pharmacology; D004716:Endometritis / Q000235:genetics / Q000276:immunology / Q000662:veterinary; D004926:Escherichia coli / Q000276:immunology* / Q000472:pathogenicity; D004927:Escherichia coli Infections / Q000235:genetics / Q000276:immunology / Q000662:veterinary; D005260:Female; D066298:In Vitro Techniques; D015850:Interleukin-6 / Q000235:genetics; D015289:Leukotrienes / Q000235:genetics / Q000276:immunology* / Q000494:pharmacology; D011453:Prostaglandins / Q000276:immunology; D011644:Puerperal Disorders / Q000235:genetics / Q000276:immunology / Q000662:veterinary; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051197:Toll-Like Receptor 4 / Q000235:genetics; D014599:Uterus / Q000187:drug effects / Q000276:immunology* / Q000382:microbiology
chemical_list: D016207:Cytokines; D015850:Interleukin-6; D015289:Leukotrienes; D011453:Prostaglandins; D012333:RNA, Messenger; D051197:Toll-Like Receptor 4
keywords: Cow; Cytokines; Inflammation; Leukotrienes; Uterus