title: The Modification of Tet1 in Male Germline Stem Cells and Interact with PCNA, HDAC1 to promote their Self-renewal and Proliferation.
abstract: Epigenetic modification plays key roles in spermatogenesis, especially DNA methylation dynamic is important in sustaining normal spermatogenesis. Ten-eleven translocation 1 (Tet1) is not only a key demethylase, which works in specific gene regions, but also crosstalks with partners to regulate epigenetic progress as protein complexes. Dairy goat is an important livestock in China, while the unstable culture system in vitro inhibits optimization of new dairy goat species. The study of epigenetic modification in male germline stem cells (mGSCs) is beneficial to the optimization of adult stem cell culture system in vitro, and the improvement of sperm quality and breeding of selected livestock. In our study, we not only analyzed the morphology, gene expression, DNA methylation and histone methylation dynamic in mouse Tet1 (mTet1) modified mGSCs, we also analyzed the stemness ability by in vivo transplantation and explored the functional mechanism of Tet1 in dairy goat mGSCs. The results showed mTet1 modified mGSCs had better self-renewal and proliferation ability than wild-type mGSCs, mTet1 could also up-regulate JMJD3 to decrease H3K27me3, which also showed to suppress the MEK-ERK pathway. Furthermore, Co-IP analysis demonstrated that TET1 interact with PCNA and HDAC1 by forming protein complexes to comprehensively regulate dairy goat mGSCs and spermatogenesis.
authors.affiliation: College of Veterinary Medicine, Shaanxi Centre of Stem Cells Engineering &Technology, Northwest A&F University, Yangling, Shaanxi, 712100 China.
authors.forename: Liming
authors.initials: L
authors.lastname: Zheng
authors.affiliation: College of Veterinary Medicine, Shaanxi Centre of Stem Cells Engineering &Technology, Northwest A&F University, Yangling, Shaanxi, 712100 China.
authors.forename: Yuanxin
authors.initials: Y
authors.lastname: Zhai
authors.affiliation: College of Veterinary Medicine, Shaanxi Centre of Stem Cells Engineering &Technology, Northwest A&F University, Yangling, Shaanxi, 712100 China.
authors.forename: Na
authors.initials: N
authors.lastname: Li
authors.affiliation: College of Veterinary Medicine, Shaanxi Centre of Stem Cells Engineering &Technology, Northwest A&F University, Yangling, Shaanxi, 712100 China.
authors.forename: Fanglin
authors.initials: F
authors.lastname: Ma
authors.affiliation: College of Veterinary Medicine, Shaanxi Centre of Stem Cells Engineering &Technology, Northwest A&F University, Yangling, Shaanxi, 712100 China.
authors.forename: Haijing
authors.initials: H
authors.lastname: Zhu
authors.affiliation: College of Veterinary Medicine, Shaanxi Centre of Stem Cells Engineering &Technology, Northwest A&F University, Yangling, Shaanxi, 712100 China.
authors.forename: Xiaomin
authors.initials: X
authors.lastname: Du
authors.affiliation: Key Laboratory for Mammalian Reproductive Biology and Biotechnology, Ministry of Education, Inner Mongolia University, Hohhot, 010021, China.
authors.forename: Guangpeng
authors.initials: G
authors.lastname: Li
authors.affiliation: College of Veterinary Medicine, Shaanxi Centre of Stem Cells Engineering &Technology, Northwest A&F University, Yangling, Shaanxi, 712100 China.
authors.forename: Jinlian
authors.initials: J
authors.lastname: Hua
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000235:genetics; D049109:Cell Proliferation / Q000235:genetics*; D000066673:Cell Self Renewal / Q000235:genetics*; D019175:DNA Methylation / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics*; D044127:Epigenesis, Genetic; D018507:Gene Expression Regulation, Developmental; D005854:Germ Cells / Q000254:growth & development; D006041:Goats / Q000235:genetics; D056284:Histone Deacetylase 1 / Q000235:genetics*; D056484:Jumonji Domain-Containing Histone Demethylases / Q000235:genetics; D020935:MAP Kinase Signaling System; D008297:Male; D051379:Mice; D018809:Proliferating Cell Nuclear Antigen / Q000235:genetics*; D011518:Proto-Oncogene Proteins / Q000235:genetics*; D013091:Spermatogenesis / Q000235:genetics; D013234:Stem Cells / Q000378:metabolism
chemical_list: D004268:DNA-Binding Proteins; D018809:Proliferating Cell Nuclear Antigen; D011518:Proto-Oncogene Proteins; C540839:TET1 protein, mouse; D056484:Jumonji Domain-Containing Histone Demethylases; C523761:Kdm6b protein, mouse; C484389:Hdac1 protein, mouse; D056284:Histone Deacetylase 1