title: Fluorescent detection of dipicolinic acid as a biomarker of bacterial spores using lanthanide-chelated gold nanoparticles.
abstract: Gold nanoparticles (GNPs) functionalized with ethylenediamine-lanthanide complexes (Eu-GNPs and Tb-GNPs) were used for the selective fluorescent detection of dipicolinic acid (DPA), a unique biomarker of bacterial spores, in water. Particles were characterized by transmission electron microscopy and zeta potential measurements. The coordination of DPA to the lanthanides resulted in the enhancement of the fluorescence. A selective response to DPA was observed over the nonselective binding of aromatic ligands. The ligand displacement strategy were also employed for the ratiometric fluorescent detection of DPA. 4,4,4-trifluoro-1-(2-naphthyl)-1,3-butanedion (TFNB) was chosen as an antenna to synthesize ternary complexes. The addition of DPA on EuGNP:TFNB ternary complex quenched the initial emission of the complex at 615nm and increased the TFNB emission at 450nm when excited at 350nm. The results demonstrated that the ratiometric fluorescent detection of DPA was achieved by ligand displacement strategy.
authors.affiliation: Department of Chemistry, Faculty of Art and Sciences, Duzce University, Duzce 81620, Turkey.
authors.forename: Mert
authors.initials: M
authors.lastname: Donmez
authors.affiliation: Department of Bioengineering, Faculty of Engineering and Architecture, Konya Food and Agriculture University, Konya 42080, Turkey. Electronic address: deniz.yilmaz@gidatarim.edu.tr.
authors.forename: M Deniz
authors.initials: MD
authors.lastname: Yilmaz
authors.affiliation: Department of Chemistry, Faculty of Art and Sciences, Duzce University, Duzce 81620, Turkey. Electronic address: benankilbas@duzce.edu.tr.
authors.forename: Benan
authors.initials: B
authors.lastname: Kilbas
mesh_terms: D015415:Biomarkers; D003931:Diacetyl / Q000031:analogs & derivatives / Q000737:chemistry; D004735:Energy Transfer; D006046:Gold / Q000737:chemistry*; D028581:Lanthanoid Series Elements / Q000737:chemistry*; D008163:Luminescent Measurements / Q000379:methods*; D053768:Metal Nanoparticles / Q000737:chemistry*; D009281:Naphthalenes / Q000737:chemistry; D010848:Picolinic Acids / Q000032:analysis*; D013171:Spores, Bacterial / Q000302:isolation & purification*; D014871:Water Microbiology / Q000592:standards
chemical_list: C575376:4,4,4 trifluoro-1-(2-naphthyl)1,3-butanedione; D015415:Biomarkers; D028581:Lanthanoid Series Elements; D009281:Naphthalenes; D010848:Picolinic Acids; D006046:Gold; D003931:Diacetyl; C004860:dipicolinic acid
keywords: Bacterial spores; Dipicolinic acid; Fluorescence; Gold nanoparticles; Lanthanide