title: Some proteins of M. tuberculosis that localise to the nucleus of THP-1-derived macrophages.
abstract: Host-pathogen dialectics in tuberculosis (TB) via DNA-protein interactions are emerging. We investigated whether proteins produced by Mycobacterium tuberculosis (Mtb) could translocate to the host nucleus. Using lysates of nuclei purified from Mtb-infected THP-1-derived macrophages, we identified at least 15 proteins of Mtb-origin by electrophoretic and chromatographic separation and mass spectrometry. Western blotting confirmed time-dependent accumulation of Mtb EF-Tu, GroEL, GroES and MtrA in the host nucleus. MtrA could pull down at least 16 host proteins. Mtb proteins may have moonlighting functions that affect host gene expression.
authors.affiliation: CSIR Central Drug Research Institute, Lucknow 226031, India.
authors.forename: Atul Kumar
authors.initials: AK
authors.lastname: Agrawal
authors.affiliation: CSIR Central Drug Research Institute, Lucknow 226031, India.
authors.forename: Rajeev
authors.initials: R
authors.lastname: Ranjan
authors.affiliation: CSIR Central Drug Research Institute, Lucknow 226031, India.
authors.forename: Sharat
authors.initials: S
authors.lastname: Chandra
authors.affiliation: CSIR Central Drug Research Institute, Lucknow 226031, India.
authors.forename: Tofan Kumar
authors.initials: TK
authors.lastname: Rout
authors.affiliation: CSIR Central Drug Research Institute, Lucknow 226031, India. Electronic address: amit_misra@cdri.res.in.
authors.forename: Amit
authors.initials: A
authors.lastname: Misra
authors.affiliation: CSIR Central Drug Research Institute, Lucknow 226031, India. Electronic address: tjreddy@cdri.res.in.
authors.forename: Thota Jagadeshwar
authors.initials: TJ
authors.lastname: Reddy
mesh_terms: D001426:Bacterial Proteins / Q000378:metabolism*; D002467:Cell Nucleus / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000378:metabolism; D054884:Host-Pathogen Interactions / Q000502:physiology; D006801:Humans; D008264:Macrophages / Q000378:metabolism* / Q000382:microbiology; D009169:Mycobacterium tuberculosis / Q000378:metabolism* / Q000502:physiology; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods; D014376:Tuberculosis / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D004268:DNA-Binding Proteins
keywords: DNA-binding proteins; Moonlighting; Nano-LC/MS; Nuclear proteome; Protein–protein interactions