title: Influenza virus infection induces translocation of apoptosis-inducing factor (AIF) in A549 cells: role of AIF in apoptosis and viral propagation.
abstract: It is recognized that influenza virus induces caspase-dependent apoptosis by activating caspase-3. Apoptosis-inducing factor (AIF) is a caspase-independent cell death effector, and its mitochondrial-nuclear translocation plays an important role in apoptosis. It is demonstrated in this study how influenza virus infection can induce caspase-independent apoptosis in the human alveolar epithelial cell line A549. AIF is translocated from the mitochondria to the nucleus in a caspase-independent manner in response to infection with influenza virus. Knockdown of AIF expression by small interfering RNA (siRNA) led to a reduction in virus-infection-induced apoptosis and virus yield. These results indicate that AIF translocation has a role in influenza-virus-induced apoptosis.
authors.affiliation: Beijing Institute of Radiation Medicine, 27 Taiping Road, Beijing, 100850, China.|Shandong Analysis and Test Center, Shandong Academy of Sciences, Jinan, China.
authors.forename: Xinyan
authors.identifier: http://orcid.org/0000-0002-9512-465X
authors.initials: X
authors.lastname: Qu
authors.affiliation: Beijing Institute of Radiation Medicine, 27 Taiping Road, Beijing, 100850, China.
authors.forename: Xiaoran
authors.initials: X
authors.lastname: Ding
authors.affiliation: Beijing Institute of Radiation Medicine, 27 Taiping Road, Beijing, 100850, China.|Institute of Zoonoses, Jilin University, 5333 Xian road, Changchun, 130062, China.
authors.forename: Ming
authors.initials: M
authors.lastname: Duan
authors.affiliation: Beijing Institute of Radiation Medicine, 27 Taiping Road, Beijing, 100850, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Yang
authors.affiliation: Beijing Institute of Radiation Medicine, 27 Taiping Road, Beijing, 100850, China.
authors.forename: Ruxian
authors.initials: R
authors.lastname: Lin
authors.affiliation: Beijing Institute of Radiation Medicine, 27 Taiping Road, Beijing, 100850, China. zhouzhe@bmi.ac.cn.
authors.forename: Zhe
authors.initials: Z
authors.lastname: Zhou
authors.affiliation: Beijing Institute of Radiation Medicine, 27 Taiping Road, Beijing, 100850, China. sqwang@bmi.ac.cn.
authors.forename: Shengqi
authors.initials: S
authors.lastname: Wang
mesh_terms: D000072283:A549 Cells; D017209:Apoptosis; D051033:Apoptosis Inducing Factor / Q000235:genetics / Q000378:metabolism*; D002467:Cell Nucleus / Q000378:metabolism; D006801:Humans; D053118:Influenza A Virus, H1N1 Subtype / Q000235:genetics / Q000254:growth & development / Q000502:physiology*; D007251:Influenza, Human / Q000235:genetics / Q000378:metabolism* / Q000821:virology; D008928:Mitochondria / Q000378:metabolism; D021381:Protein Transport
chemical_list: D051033:Apoptosis Inducing Factor