title: The biochemical characterization, stabilization studies and the antiproliferative effect of bromelain against B16F10 murine melanoma cells.
abstract: The current study aims to extract bromelain from different parts (stem, crown, peels, pulp and leaves) of Ananas comosus var. comosus AGB 772; to determine of optimum pH and temperature; to test bromelain stability in disodium EDTA and sodium benzoate, and to investigate its pharmacological activity on B16F10 murine melanoma cells in vitro. The highest enzymatic activity was found in bromelain extracted from the pulp and peel. The optimum bromelain pH among all studied pineapple parts was 6.0. The optimum temperature was above 50 °C in all bromelain extracts. The fluorescence analysis confirmed the stability of bromelain in the presence of EDTA and sodium benzoate. Bromelain was pharmacologically active against B16F10 melanoma cells and it was possible verifying approximately 100% inhibition of tumor cell proliferation in vitro. Since bromelain activity was found in different parts of pineapple plants, pineapple residues from the food industry may be used for bromelain extraction.
authors.affiliation: a Health Department , State University of Feira de Santana (UEFS) , Feira de Santana , Brazil.
authors.forename: Íngara Keisle
authors.initials: ÍK
authors.lastname: São Paulo Barretto Miranda
authors.affiliation: b Parasitic Biology Laboratory , Oswaldo Cruz Foundation (FIOCRUZ) , Salvador , Brazil.
authors.forename: Anderson
authors.initials: A
authors.lastname: Fontes Suzart Miranda
authors.affiliation: c Embrapa Mandioca e Fruticultura , Cruz das Almas , Brazil.
authors.forename: Fernanda Vidigal Duarte
authors.initials: FV
authors.lastname: Souza
authors.affiliation: b Parasitic Biology Laboratory , Oswaldo Cruz Foundation (FIOCRUZ) , Salvador , Brazil.
authors.forename: Marcos André
authors.initials: MA
authors.lastname: Vannier-Santos
authors.affiliation: d Biological Sciences Department , Biotechnology and Genetics Center, State University of Santa Cruz , Ilhéus , Brazil.
authors.forename: Carlos Priminho
authors.initials: CP
authors.lastname: Pirovani
authors.affiliation: e Laboratory of Optical Properties, Physics Department , Federal University of Bahia , Salvador , Brazil.
authors.forename: Iuri Muniz
authors.initials: IM
authors.lastname: Pepe
authors.affiliation: e Laboratory of Optical Properties, Physics Department , Federal University of Bahia , Salvador , Brazil.
authors.forename: Ivanoé João
authors.initials: IJ
authors.lastname: Rodowanski
authors.affiliation: d Biological Sciences Department , Biotechnology and Genetics Center, State University of Santa Cruz , Ilhéus , Brazil.
authors.forename: Katiúcia Tícila de Souza Eduvirgens
authors.initials: KT
authors.lastname: Ferreira
authors.affiliation: f Technology, Sanitation, Hydric Resources and Environment Department , State University of Feira de Santana (UEFS) , Feira de Santana , Brazil.
authors.forename: Luciano
authors.initials: L
authors.lastname: Mendes Souza Vaz
authors.affiliation: a Health Department , State University of Feira de Santana (UEFS) , Feira de Santana , Brazil.
authors.forename: Sandra Aparecida
authors.initials: SA
authors.lastname: de Assis
mesh_terms: D031230:Ananas / Q000737:chemistry*; D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000494:pharmacology*; D001963:Bromelains / Q000737:chemistry / Q000494:pharmacology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D051379:Mice; D035261:Plant Components, Aerial / Q000737:chemistry
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D001963:Bromelains
keywords: Antiproliferative effect in vitro; bromelain; cell culture; enzyme stability; murine melanoma cells; response surface methodology