title: Effect of plant-based carbon sources on denitrifying microorganisms in a vertical flow constructed wetland.
abstract: The effects of supplementing plant-based carbon sources, fermented tissues of Arundo donax and Pontederia cordata, and a combination of the two plants, on the nitrogen removal efficiency and microbial composition in a vertical flow constructed wetland (VFCW) were examined. The results showed that the addition of the composite carbon source produced the highest removal efficiencies of NH4+-N 91.5%, NO3--N 94.5% and TN 92.8% in VFCW. The detected abundance of amoA, nirS, and nxrA genes indicated that ammonia oxidation bacteria and denitrifying bacteria were more abundant than the nitrite oxidation bacteria. Furthermore, the addition of the composite carbon source significantly promoted the growth of the denitrifying bacteria in VFCW. The results indicated that supplementing the system with plant-based carbon sources achieved partial nitrification and denitrification, as well as classic denitrification in VFCWs. The study suggested that multiple nitrogen removal pathways were required to feasibly and efficiently remove nitrogen.
authors.affiliation: Shenzhen Key Laboratory of Marine Bioresource and Eco-environmental Science, College of Life Sciences and Oceanography, Shenzhen University, Shenzhen 518060, China. Electronic address: fugp@szu.edu.cn.
authors.forename: Guiping
authors.initials: G
authors.lastname: Fu
authors.affiliation: Shenzhen Key Laboratory of Marine Bioresource and Eco-environmental Science, College of Life Sciences and Oceanography, Shenzhen University, Shenzhen 518060, China.
authors.forename: Linkun
authors.initials: L
authors.lastname: Huangshen
authors.affiliation: Shenzhen Key Laboratory of Marine Bioresource and Eco-environmental Science, College of Life Sciences and Oceanography, Shenzhen University, Shenzhen 518060, China.
authors.forename: Zhipeng
authors.initials: Z
authors.lastname: Guo
authors.affiliation: State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China.
authors.forename: Qiaohong
authors.initials: Q
authors.lastname: Zhou
authors.affiliation: State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China.
authors.forename: Zhenbin
authors.initials: Z
authors.lastname: Wu
mesh_terms: D000641:Ammonia / Q000378:metabolism; D001419:Bacteria / Q000235:genetics / Q000378:metabolism; D002244:Carbon / Q000378:metabolism; D058440:Denitrification; D059013:Microbial Consortia / Q000235:genetics / Q000502:physiology*; D009566:Nitrates / Q000378:metabolism; D058465:Nitrification; D009584:Nitrogen / Q000302:isolation & purification / Q000378:metabolism; D006109:Poaceae / Q000378:metabolism; D014865:Waste Disposal, Fluid / Q000379:methods*; D053833:Wetlands
chemical_list: D009566:Nitrates; D002244:Carbon; D000641:Ammonia; D009584:Nitrogen
keywords: Gene abundance; Nitrogen removal pathway; Partial nitrification-denitrification; Plant-based carbon source; Vertical flow constructed wetland