title: The potential application and challenge of powerful CRISPR/Cas9 system in cardiovascular research.
abstract: CRISPR/Cas9 is a precision-guided munition found in bacteria to fight against invading viruses. This technology has enormous potential applications, including altering genes in both somatic and germ cells, as well as generating knockout animals. Compared to other gene editing techniques such as zinc finger nucleases and TALENS, CRISPR/Cas9 is much easier to use and highly efficient. Importantly, the multiplex capacity of this technology allows multiple genes to be edited simultaneously. CRISPR/Cas9 also has the potential to prevent and cure human diseases. In this review, we wish to highlight some key points regarding the future prospect of using CRISPR/Cas9 as a powerful tool for cardiovascular research, and as a novel therapeutic strategy to treat cardiovascular diseases.
authors.affiliation: Institute for Cardiovascular Science & Department of Cardiovascular Surgery, First Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215123, PR China.. Electronic address: yangxin_li@yahoo.com.
authors.forename: Yangxin
authors.initials: Y
authors.lastname: Li
authors.affiliation: Cyrus Tang Hematology Center, Jiangsu Institute of Hematology, First Affiliated Hospital, Collaborative Innovation Center of Hematology, Soochow University, Suzhou, Jiangsu 215123, PR China.
authors.forename: Yao-Hua
authors.initials: YH
authors.lastname: Song
authors.affiliation: Department of Cardiology, Second Hospital of Jilin University, Changchun, Jilin .130041, PR China.
authors.forename: Bin
authors.initials: B
authors.lastname: Liu
authors.affiliation: Guangdong Cardiovascular Institute, Guangzhou Medical University, Guangzhou, Guangdong 510080, PR China.
authors.forename: Xi-Yong
authors.initials: XY
authors.lastname: Yu
mesh_terms: D000818:Animals; D035843:Biomedical Research / Q000379:methods / Q000639:trends*; D064113:CRISPR-Cas Systems; D002318:Cardiovascular Diseases / Q000175:diagnosis / Q000235:genetics* / Q000628:therapy*; D064112:Clustered Regularly Interspaced Short Palindromic Repeats / Q000235:genetics*; D005544:Forecasting; D000072669:Gene Editing; D018390:Gene Targeting / Q000379:methods*; D015316:Genetic Therapy / Q000379:methods; D006801:Humans; D015533:Transcriptional Activation
keywords: CRISPR/Cas9; Cardiovascular research; Gene regulation; Gene therapy; Stem cell