title: Inhibitory activities of Cystoseira crinita sulfated polysaccharide on key enzymes related to diabetes and hypertension: in vitro and animal study.
abstract: The present study investigated the effect of the Cystoseira crinita sulfated polysaccharide (CCSP) on key enzymes activities related to diabetes in vitro and in diabetic rats. We found that CCSP inhibited pancreatic α-amylase with IC50= 39.16 μg/ml and angiotensin I-converting enzyme (ACE) activity with IC50=58.35 μg/ml in vitro. In diabetic rats, the administration of CCSP reduced the activity of α-amylase in serum, pancreas, and intestine by 23%, 44.38%, and 45%, respectively as compared to untreated diabetic rats. Moreover, the administration of CCSP to surviving diabetic rats protects pancreas β cells from death and damage, which leads to insulin levels. The decrease in α-amylase and the increase in insulin level lead to a decrease in glucose rate by 56% as compared to untreated diabetic rats. The inhibitory action of α-amylase activity and hypoglycemic effect of CCSP were confirmed by oral glucose tolerance test (OGTT). In addition, the administration of CCSP to surviving diabetic rats normalizes lipid profile, stimulates antioxidant capacity, and prevents liver-kidney toxicities, evidenced by decrease in serum indices of liver and kidney toxicity and confirmed by histological analysis. The overall findings presented in this study demonstrate that the administration of CCSP to diabetic rats can make it a potentially strong candidate for industrial application as a pharmacological agent for the treatment of hyperglycemia, hyperlipidemia, and liver-kidney dysfunctions.
authors.affiliation: a Laboratory of Plant Biotechnology Applied to the Improvement of Cultures , Faculty of Sciences of Sfax , Sfax , Tunisia.|b Laboratory of Animal Ecophysiology , Faculty of Sciences of Sfax , Sfax , Tunisia.
authors.forename: Amel
authors.initials: A
authors.lastname: Ben Gara
authors.affiliation: a Laboratory of Plant Biotechnology Applied to the Improvement of Cultures , Faculty of Sciences of Sfax , Sfax , Tunisia.
authors.forename: Rihab
authors.initials: R
authors.lastname: Ben Abdallah Kolsi
authors.affiliation: c Histology, Orthopaedic and Traumatology Laboratory, Faculty of Medicine of Sfax , Sfax , Tunisia.
authors.forename: Neila
authors.initials: N
authors.lastname: Jardak
authors.affiliation: d Biochemistry Laboratory, CHU Hedi Chaker , Sfax , Tunisia , and.
authors.forename: Rim
authors.initials: R
authors.lastname: Chaaben
authors.affiliation: b Laboratory of Animal Ecophysiology , Faculty of Sciences of Sfax , Sfax , Tunisia.
authors.forename: Abdelfattah
authors.initials: A
authors.lastname: El-Feki
authors.affiliation: a Laboratory of Plant Biotechnology Applied to the Improvement of Cultures , Faculty of Sciences of Sfax , Sfax , Tunisia.
authors.forename: Lotfi
authors.initials: L
authors.lastname: Fki
authors.affiliation: e Laboratory of Molecular Biology of Eukaryotes Center of Biotechnology of Sfax , Sfax , Tunisia.
authors.forename: Hafedh
authors.initials: H
authors.lastname: Belghith
authors.affiliation: a Laboratory of Plant Biotechnology Applied to the Improvement of Cultures , Faculty of Sciences of Sfax , Sfax , Tunisia.
authors.forename: Karima
authors.initials: K
authors.lastname: Belghith
mesh_terms: D000806:Angiotensin-Converting Enzyme Inhibitors / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism / Q000627:therapeutic use; D000818:Animals; D000959:Antihypertensive Agents / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism / Q000627:therapeutic use*; D001688:Biological Products / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism / Q000627:therapeutic use; D003924:Diabetes Mellitus, Type 2 / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology; D004791:Enzyme Inhibitors / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism / Q000627:therapeutic use*; D006973:Hypertension / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology; D007004:Hypoglycemic Agents / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism / Q000627:therapeutic use*; D007413:Intestinal Mucosa / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism; D007421:Intestine, Small / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism; D007668:Kidney / Q000187:drug effects / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology; D008099:Liver / Q000187:drug effects / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology; D008297:Male; D008522:Mediterranean Sea; D010179:Pancreas / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D055292:Pancreatic alpha-Amylases / Q000037:antagonists & inhibitors / Q000097:blood / Q000378:metabolism; D007703:Peptidyl-Dipeptidase A / Q000737:chemistry / Q000378:metabolism; D000459:Phaeophyceae / Q000737:chemistry* / Q000254:growth & development; D011134:Polysaccharides / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism / Q000627:therapeutic use*; D017208:Rats, Wistar; D014416:Tunisia
chemical_list: D000806:Angiotensin-Converting Enzyme Inhibitors; D000959:Antihypertensive Agents; D001688:Biological Products; D004791:Enzyme Inhibitors; D007004:Hypoglycemic Agents; D011134:Polysaccharides; D055292:Pancreatic alpha-Amylases; D007703:Peptidyl-Dipeptidase A
keywords: ACE; Diabetes; liver-kidney functions; pancreas; polysaccharide