title: Effects of hPTPβ inhibitor on microcirculation of rat cremaster muscle flap following ischemia-reperfusion injury.
abstract: BACKGROUND
  Inhibition of protein tyrosine phosphatases (PTP) enhances endothelial receptor tyrosine kinases activation and may have beneficial effects on vessel growth and improve blood flow to ischemic tissue. The purpose of this study is to determine influence of hPTPß inhibitors on ischemia-reperfusion injury in muscle flap.
  
  
  METHODS
  Following cremaster muscle dissection, 60 rats divided into 10 experimental groups (placebo and treatment groups following 0, 1, 2, 3, and 4 h of ischemia). Following group-specific treatment (placebo/hPTPß inhibitor, 15 mg/kg), 2 h of reperfusion is initiated. Observations are performed at 4 h after completion of reperfusion and microcirculatory hemodynamics and leukocyte-endothelial activation were recorded.
  
  
  RESULTS
  Administration of hPTPß inhibitor showed preservation of capillary perfusion in group subjected to 2 h of ischemia when compared with placebo (P < .05). The effect of hPTPβ inhibitor on mean venule diameter was found to be altered by duration of ischemia and this effect was statistically significant (P < .05). Treated ischemic groups (1 h, 2 h, and 3 h) showed decreased activation of rolling, sticking, and transmigrating leukocytes compared to respective placebo groups at all time points. The differences were significant for transmigrating leukocytes after 2 h and 3 h of ischemia (P < .05). Endothelial edema index was also significantly reduced in 2 h ischemia group (P < .05).
  
  
  CONCLUSIONS
  Administration of hPTP inhibitors after submission of tissue to subcritical ischemia (1-2 h) improved functional capillary perfusion and decreased leukocyte-endothelial activation after 4 h after reperfusion. These results indicate that hPTP inhibitor has a potential postischemic therapeutic effect applied after tissue ischemia just before the reperfusion injury.
authors.affiliation: Department of Plastic Surgery, Gulhane Military Medical Academy, Ankara, Turkey.
authors.forename: Fatih
authors.initials: F
authors.lastname: Zor
authors.affiliation: Department of Plastic Surgery, Cleveland Clinic, Cleveland, OH.|Klinik für Plastische, Ästhetische und Rekonstruktive Chirurgie, Rote Kreuz Krankenhaus, Kassel, Germany.
authors.forename: Cem
authors.initials: C
authors.lastname: Meric
authors.affiliation: Department of Plastic Surgery, Cleveland Clinic, Cleveland, OH.|Department of Orthopedics, University of Illinois at Chicago, Chicago, IL.
authors.forename: Maria
authors.initials: M
authors.lastname: Siemionow
mesh_terms: D000009:Abdominal Muscles / Q000601:surgery* / Q000637:transplantation; D000704:Analysis of Variance; D000818:Animals; D001783:Blood Flow Velocity / Q000187:drug effects; D004195:Disease Models, Animal; D008297:Male; D008833:Microcirculation / Q000187:drug effects*; D017027:Protein Tyrosine Phosphatases / Q000037:antagonists & inhibitors* / Q000494:pharmacology; D011897:Random Allocation; D051381:Rats; D011917:Rats, Inbred Lew; D012016:Reference Values; D015427:Reperfusion Injury / Q000188:drug therapy*; D018709:Statistics, Nonparametric; D013524:Surgical Flaps / Q000098:blood supply*
chemical_list: D017027:Protein Tyrosine Phosphatases
keywords: hPTPβ inhibitor; ischemia-reperfusion injury; microcirculation; protein tyrosine phosphatase; receptor tyrosine kinase