title: Increased Activity and Apoptosis of Eosinophils in Blister Fluids, Skin and Peripheral Blood of Patients with Bullous Pemphigoid.
abstract: Bullous pemphigoid (BP) is an autoimmune blistering skin disease that is more common in elderly individuals. The aim of this study was to determine the functional activity of eosinophils in patients with BP compared with healthy donors. Blood, skin and blister-derived eosinophils were strongly activated in patients with BP, seen by increased surface expression of CD69 compared with controls. CD11b was also increased in BP blood eosinophils, which may explain the striking accumulation of eosinophils in BP (1×106 per ml blister fluid). Furthermore, CCL26 was expressed by activated eosinophils in BP skin and in blister fluid. BP eosinophils also released IL-6, IL-8 and IL-1α in BP blister fluids. Apoptosis in cultivated BP eosinophils was increased and accompanied by enhanced surface externalization of CD95. Caspase 3 positive eosinophils in lesional BP skin and blister fluid also showed the initiation of apoptosis. These results reveal novel pathophysiological aspects of BP, with a strong activation pattern and increased apoptosis of eosinophils in the peripheral blood, skin and blister fluids.
authors.affiliation: Department of Dermatology and Allergy, Hannover Medical School, DE-30625 Hannover, Germany.
authors.forename: Judith
authors.initials: J
authors.lastname: Engmann
authors.forename: Urda
authors.initials: U
authors.lastname: Rüdrich
authors.forename: Georg
authors.initials: G
authors.lastname: Behrens
authors.forename: Eleni
authors.initials: E
authors.lastname: Papakonstantinou
authors.forename: Manuela
authors.initials: M
authors.lastname: Gehring
authors.forename: Alexander
authors.initials: A
authors.lastname: Kapp
authors.forename: Ulrike
authors.initials: U
authors.lastname: Raap
mesh_terms: D015703:Antigens, CD / Q000097:blood; D000945:Antigens, Differentiation, T-Lymphocyte / Q000097:blood; D017209:Apoptosis; D015415:Biomarkers / Q000097:blood; D001768:Blister / Q000097:blood / Q000276:immunology / Q000473:pathology*; D039481:CD11b Antigen / Q000097:blood; D016022:Case-Control Studies; D053148:Caspase 3 / Q000378:metabolism; D000075906:Chemokine CCL26; D019742:Chemokines, CC / Q000378:metabolism; D004804:Eosinophils / Q000276:immunology / Q000378:metabolism / Q000473:pathology*; D006801:Humans; D053583:Interleukin-1beta / Q000378:metabolism; D015850:Interleukin-6 / Q000378:metabolism; D016209:Interleukin-8 / Q000378:metabolism; D037181:Lectins, C-Type / Q000097:blood; D010391:Pemphigoid, Bullous / Q000097:blood / Q000276:immunology / Q000473:pathology*; D012867:Skin / Q000276:immunology / Q000378:metabolism / Q000473:pathology*; D019014:fas Receptor / Q000378:metabolism; D000099192:CD69 Antigens
chemical_list: D015703:Antigens, CD; D000945:Antigens, Differentiation, T-Lymphocyte; D015415:Biomarkers; D039481:CD11b Antigen; D053148:Caspase 3; D000075906:Chemokine CCL26; D019742:Chemokines, CC; D053583:Interleukin-1beta; D015850:Interleukin-6; D016209:Interleukin-8; D037181:Lectins, C-Type; D019014:fas Receptor; D000099192:CD69 Antigens; C120429:CCL26 protein, human; C508606:CXCL8 protein, human; C491901:FAS protein, human; C508585:IL1B protein, human; C508600:IL6 protein, human; C509295:ITGAM protein, human; C505407:CASP3 protein, human