title: Isoprene Production on Enzymatic Hydrolysate of Peanut Hull Using Different Pretreatment Methods.
abstract: The present study is about the use of peanut hull for isoprene production. In this study, two pretreatment methods, hydrogen peroxide-acetic acid (HPAC) and popping, were employed prior to enzymatic hydrolysis, which could destroy the lignocellulosic structure and accordingly improve the efficiency of enzymatic hydrolysis. It is proven that the isoprene production on enzymatic hydrolysate with HPAC pretreatment is about 1.9-fold higher than that of popping pretreatment. Moreover, through High Performance Liquid Chromatography (HPLC) analysis, the amount and category of inhibitors such as formic acid, acetic acid, and HMF were assayed and were varied in different enzymatic hydrolysates, which may be the reason leading to a decrease in isoprene production during fermentation. To further increase the isoprene yield, the enzymatic hydrolysate of HPAC was detoxified by activated carbon. As a result, using the detoxified enzymatic hydrolysate as the carbon source, the engineered strain YJM21 could accumulate 297.5 mg/L isoprene, which accounted for about 90% of isoprene production by YJM21 fermented on pure glucose (338.6 mg/L). This work is thought to be the first attempt on isoprene production byE. coliusing peanut hull as the feedstock. More importantly, it also shows the prospect of peanut hull to be considered as an alternative feedstock for bio-based chemicals or biofuels production due to its easy access and high polysaccharide content.
authors.affiliation: Key Lab of Plant Biotechnology in Universities of Shandong Province, College of Life Sciences, Qingdao Agricultural University, Qingdao 266109, China.
authors.forename: Sumeng
authors.initials: S
authors.lastname: Wang
authors.affiliation: Key Lab of Plant Biotechnology in Universities of Shandong Province, College of Life Sciences, Qingdao Agricultural University, Qingdao 266109, China.
authors.forename: Ruichao
authors.initials: R
authors.lastname: Li
authors.affiliation: Key Lab of Plant Biotechnology in Universities of Shandong Province, College of Life Sciences, Qingdao Agricultural University, Qingdao 266109, China.
authors.forename: Xiaohua
authors.initials: X
authors.lastname: Yi
authors.affiliation: Key Lab of Plant Biotechnology in Universities of Shandong Province, College of Life Sciences, Qingdao Agricultural University, Qingdao 266109, China.
authors.forename: Tigao
authors.initials: T
authors.lastname: Fang
authors.affiliation: Key Lab of Plant Biotechnology in Universities of Shandong Province, College of Life Sciences, Qingdao Agricultural University, Qingdao 266109, China.
authors.forename: Jianming
authors.identifier: 0000-0002-3268-211X
authors.initials: J
authors.lastname: Yang
authors.affiliation: Bio-Energy Research Institute, Chonnam National University, Gwangju 500-757, Republic of Korea.
authors.forename: Hyeun-Jong
authors.identifier: 0000-0003-3184-3425
authors.initials: HJ
authors.lastname: Bae
mesh_terms: D010367:Arachis / Q000737:chemistry*; D001709:Biotechnology; D002070:Butadienes; D002480:Cellulase / Q000378:metabolism; D043364:Endo-1,4-beta Xylanases / Q000378:metabolism; D005285:Fermentation; D008401:Gas Chromatography-Mass Spectrometry; D045782:Hemiterpenes / Q000096:biosynthesis*; D006868:Hydrolysis; D008031:Lignin / Q000378:metabolism; D010420:Pentanes; D014866:Waste Products / Q000032:analysis
chemical_list: D002070:Butadienes; D045782:Hemiterpenes; D010420:Pentanes; D014866:Waste Products; C005059:isoprene; C036909:lignocellulose; D008031:Lignin; D002480:Cellulase; D043364:Endo-1,4-beta Xylanases