title: Tracing Males From Different Continents by Genotyping JC Polyomavirus in DNA From Semen Samples.
abstract: The human JC polyomavirus (JCPyV) is an ubiquitous viral agent infecting approximately 60% of humans. Recently, JCPyV sequences have been detected in semen samples. The aim of this investigation was to test whether semen JCPyV genotyping can be employed to trace the origin continent of males. Semen DNA samples (n = 170) from males of different Continents were investigated by PCR for the polymorphic JCPyV viral capsid protein 1 (VP1) sequences, followed by DNA sequencing. JCPyV sequences were detected with an overall prevalence of 27.6% (47/170). DNA sequencing revealed that European males carried JCPyV types 1A (71.4%), 4 (11.4%), 2B (2.9%), 2D1 (2.9%), and 3A (2.9%). Asians JCPyV type 2D1 (66.7%) and Africans JCPyV types 3A (33.3%) and 1A (33.3%). In 10.6% of males, two different JCPyV genotypes were detected, suggesting that the second JCPyV genotype was acquired in the destination country. This study indicates that the majority of semen samples found to be JCPyV-positive, were infected with the JCPyV genotype found in the geographic area of male origin. Therefore, semen JCPyV genotyping could be employed to trace the origin continent of males. Our findings could be applied to forensic investigations, in case of for instance sexual crimes. Indeed, JCPyV genotyping should enable investigators to make additional detailed profiling of the offender. J. Cell. Physiol. 232: 982-985, 2017. © 2016 Wiley Periodicals, Inc.
authors.affiliation: Department of Morphology, Surgery and Experimental Medicine, Section of Pathology, Oncology Experimental Biology, Laboratories of Cell Biology and Molecular Genetics, Ferrara, Italy.
authors.forename: John Charles
authors.initials: JC
authors.lastname: Rotondo
authors.affiliation: Department of Morphology, Surgery and Experimental Medicine, Section of Obstetrics and Gynaecology, Ferrara, Italy.
authors.forename: Tommaso
authors.initials: T
authors.lastname: Candian
authors.affiliation: Department of Medical Sciences, Section of Microbiology and Medical Genetics, School of Medicine, University of Ferrara, Ferrara, Italy.
authors.forename: Rita
authors.initials: R
authors.lastname: Selvatici
authors.affiliation: Department of Morphology, Surgery and Experimental Medicine, Section of Pathology, Oncology Experimental Biology, Laboratories of Cell Biology and Molecular Genetics, Ferrara, Italy.
authors.forename: Elisa
authors.initials: E
authors.lastname: Mazzoni
authors.affiliation: Department of Morphology, Surgery and Experimental Medicine, Section of Obstetrics and Gynaecology, Ferrara, Italy.
authors.forename: Gloria
authors.initials: G
authors.lastname: Bonaccorsi
authors.affiliation: Department of Morphology, Surgery and Experimental Medicine, Section of Obstetrics and Gynaecology, Ferrara, Italy.
authors.forename: Pantaleo
authors.initials: P
authors.lastname: Greco
authors.affiliation: Department of Morphology, Surgery and Experimental Medicine, Section of Pathology, Oncology Experimental Biology, Laboratories of Cell Biology and Molecular Genetics, Ferrara, Italy.
authors.forename: Mauro
authors.initials: M
authors.lastname: Tognon
authors.affiliation: Department of Morphology, Surgery and Experimental Medicine, Section of Pathology, Oncology Experimental Biology, Laboratories of Cell Biology and Molecular Genetics, Ferrara, Italy.
authors.forename: Fernanda
authors.initials: F
authors.lastname: Martini
mesh_terms: D000328:Adult; D001483:Base Sequence; D004279:DNA, Viral / Q000235:genetics*; D005838:Genotype; D060005:Genotyping Techniques / Q000379:methods*; D006801:Humans; D038622:Internationality; D007577:JC Virus / Q000235:genetics*; D008297:Male; D012661:Semen / Q000821:virology*; D016415:Sequence Alignment
chemical_list: D004279:DNA, Viral