title: Physarum polycephalum for Studying the Function of Histone Modifications In Vivo.
abstract: Histone modifications have been widely correlated with genetic activities. However, how these posttranslational modifications affect the dynamics and the structure of chromatin is poorly understood. Here, we describe the incorporation of the exogenous histone proteins into the slime mold Physarum polycephalum, which has been revealed to be a valuable tool for examining different facets of the function histones in chromatin dynamics like replication-coupled chromatin assembly, histone exchange, and nucleosome turnover.
authors.affiliation: UMR CNRS 6286 UFIP, Université de Nantes, Epigénétique: Prolifération et Différenciation, 2 rue de Houssinière, 44322, Nantes Cedex 03, France.
authors.forename: Vanessa
authors.initials: V
authors.lastname: Menil-Philippot
authors.affiliation: UMR CNRS 6286 UFIP, Université de Nantes, Epigénétique: Prolifération et Différenciation, 2 rue de Houssinière, 44322, Nantes Cedex 03, France. Christophe.Thiriet@univ-nantes.fr.
authors.forename: Christophe
authors.initials: C
authors.lastname: Thiriet
mesh_terms: D002843:Chromatin / Q000378:metabolism; D042002:Chromatin Assembly and Disassembly / Q000235:genetics / Q000502:physiology; D004261:DNA Replication / Q000235:genetics / Q000502:physiology; D042421:Histone Code / Q000235:genetics / Q000502:physiology; D006657:Histones / Q000378:metabolism*; D009707:Nucleosomes / Q000378:metabolism; D016839:Physarum polycephalum / Q000235:genetics / Q000378:metabolism*; D011499:Protein Processing, Post-Translational / Q000235:genetics / Q000502:physiology
chemical_list: D002843:Chromatin; D006657:Histones; D009707:Nucleosomes
keywords: Assembly; Chromatin; Exchange; Histone modification; Nucleosome; Physarum polycephalum; Turnover