title: Comparative Proteomic Analysis of Light-Induced Mycelial Brown Film Formation inLentinula edodes.
abstract: Light-induced brown film (BF) formation by the vegetative mycelium ofLentinula edodesis important for ensuring the quantity and quality of this edible mushroom. Nevertheless, the molecular mechanism underlying this phenotype is still unclear. In this study, a comparative proteomic analysis of mycelial BF formation inL. edodeswas performed. Seventy-three protein spots with at least a twofold difference in abundance on two-dimensional electrophoresis (2DE) maps were observed, and 52 of them were successfully identified by matrix-assisted laser desorption/ionization tandem time-of-flight mass spectrometry (MALDI-TOF/TOF/MS). These proteins were classified into the following functional categories: small molecule metabolic processes (39%), response to oxidative stress (5%), and organic substance catabolic processes (5%), followed by oxidation-reduction processes (3%), single-organism catabolic processes (3%), positive regulation of protein complex assembly (3%), and protein metabolic processes (3%). Interestingly, four of the proteins that were upregulated in response to light exposure were nucleoside diphosphate kinases. To our knowledge, this is the first proteomic analysis of the mechanism of BF formation inL. edodes. Our data will provide a foundation for future detailed investigations of the proteins linked to BF formation.
authors.affiliation: National Engineering Research Centre of Edible Fungi, Key Laboratory of Edible Fungi Resources and Utilization (South), Ministry of Agriculture, Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Shanghai 201403, China; State Key Laboratory of Microbial Metabolism, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China.
authors.forename: Li Hua
authors.initials: LH
authors.lastname: Tang
authors.affiliation: National Engineering Research Centre of Edible Fungi, Key Laboratory of Edible Fungi Resources and Utilization (South), Ministry of Agriculture, Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Shanghai 201403, China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Tan
authors.affiliation: National Engineering Research Centre of Edible Fungi, Key Laboratory of Edible Fungi Resources and Utilization (South), Ministry of Agriculture, Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Shanghai 201403, China.
authors.forename: Da Peng
authors.initials: DP
authors.lastname: Bao
authors.affiliation: State Key Laboratory of Microbial Metabolism, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China.
authors.forename: Xue Hong
authors.initials: XH
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Microbial Metabolism, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China.
authors.forename: Hua Hua
authors.initials: HH
authors.lastname: Jian
authors.affiliation: National Engineering Research Centre of Edible Fungi, Key Laboratory of Edible Fungi Resources and Utilization (South), Ministry of Agriculture, Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Shanghai 201403, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Li
authors.affiliation: National Engineering Research Centre of Edible Fungi, Key Laboratory of Edible Fungi Resources and Utilization (South), Ministry of Agriculture, Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Shanghai 201403, China.
authors.forename: Rui Heng
authors.initials: RH
authors.lastname: Yang
authors.affiliation: National Engineering Research Centre of Edible Fungi, Key Laboratory of Edible Fungi Resources and Utilization (South), Ministry of Agriculture, Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Shanghai 201403, China.
authors.forename: Ying
authors.identifier: 0000-0002-0580-4337
authors.initials: Y
authors.lastname: Wang
mesh_terms: D000363:Agaricales / Q000235:genetics* / Q000528:radiation effects; D018441:Biofilms / Q000254:growth & development / Q000528:radiation effects; D005656:Fungal Proteins / Q000096:biosynthesis*; D015966:Gene Expression Regulation, Fungal / Q000528:radiation effects; D008027:Light; D018384:Oxidative Stress / Q000235:genetics / Q000528:radiation effects; D040901:Proteomics; D020942:Shiitake Mushrooms / Q000235:genetics / Q000378:metabolism* / Q000528:radiation effects; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D005656:Fungal Proteins