title: Genetic and biochemical analysis reveals linked QTLs determining natural variation for fruit post-harvest water loss in pepper (Capsicum).
abstract: Molecular markers linked to QTLs controlling post-harvest fruit water loss in pepper may be utilized to accelerate breeding for improved shelf life and inhibit over-ripening before harvest. Bell pepper (Capsicum annuum L.) is an important vegetable crop world-wide. However, marketing is limited by the relatively short shelf life of the fruit due to water loss and decay that occur during prolonged storage. Towards breeding pepper with reduced fruit post-harvest water loss (PWL), we studied the genetic, physiological and biochemical basis for natural variation of PWL. We performed quantitative trait locus (QTL) mapping of fruit PWL in multiple generations of an interspecific cross of pepper, which resulted in the identification of two linked QTLs on chromosome 10 that control the trait. We further developed near-isogenic lines (NILs) for characterization of the QTL effects. Transcriptome analysis of the NILs allowed the identification of candidate genes associated with fruit PWL-associated traits such as cuticle biosynthesis, cell wall metabolism and fruit ripening. Significant differences in PWL between the NILs in the immature fruit stage, differentially expressed cuticle-associated genes and differences in the content of specific chemical constituents of the fruit cuticle, indicated a likely influence of cuticle composition on the trait. Reduced PWL in the NILs was associated with delayed over-ripening before harvest, low total soluble solids before storage, and reduced fruit softening after storage. Our study enabled a better understanding of the genetic and biological processes controlling natural variation in fruit PWL in pepper. Furthermore, the genetic materials and molecular markers developed in this study may be utilized to breed peppers with improved shelf life and inhibited over-ripening before harvest.
authors.affiliation: Institute of Plant Science, Agricultural Research Organization, The Volcani Center, P.O. Box 15159, 7528809, Rishon Lezion, Israel.|Robert H. Smith, Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, 76100, Rehovot, Israel.
authors.forename: Sigal
authors.initials: S
authors.lastname: Popovsky-Sarid
authors.affiliation: Institute of Plant Science, Agricultural Research Organization, The Volcani Center, P.O. Box 15159, 7528809, Rishon Lezion, Israel.
authors.forename: Yelena
authors.initials: Y
authors.lastname: Borovsky
authors.affiliation: Institute of Plant Science, Agricultural Research Organization, The Volcani Center, P.O. Box 15159, 7528809, Rishon Lezion, Israel.
authors.forename: Adi
authors.initials: A
authors.lastname: Faigenboim
authors.affiliation: Department of Horticulture and Landscape Architecture, Purdue University, West Lafayette, IN, 47907, USA.
authors.forename: Eugene P
authors.initials: EP
authors.lastname: Parsons
authors.affiliation: US Arid Land Agricultural Research Center, Maricopa, AZ, 85138, USA.
authors.forename: Gregory T
authors.initials: GT
authors.lastname: Lohrey
authors.affiliation: Institute of Plant Science, Agricultural Research Organization, The Volcani Center, P.O. Box 15159, 7528809, Rishon Lezion, Israel.
authors.forename: Sharon
authors.initials: S
authors.lastname: Alkalai-Tuvia
authors.affiliation: Institute of Plant Science, Agricultural Research Organization, The Volcani Center, P.O. Box 15159, 7528809, Rishon Lezion, Israel.
authors.forename: Elazar
authors.initials: E
authors.lastname: Fallik
authors.affiliation: Division of Plant and Soil Sciences, Davis College of Agriculture, Natural Resources and Design, West Virginia University, 1090 Agricultural Sciences Building, Morgantown, WV, 26506-6108, USA.
authors.forename: Matthew A
authors.initials: MA
authors.lastname: Jenks
authors.affiliation: Institute of Plant Science, Agricultural Research Organization, The Volcani Center, P.O. Box 15159, 7528809, Rishon Lezion, Israel. iparan@volcani.agri.gov.il.
authors.forename: Ilan
authors.identifier: http://orcid.org/0000-0003-2742-3049
authors.initials: I
authors.lastname: Paran
mesh_terms: D002212:Capsicum / Q000737:chemistry / Q000235:genetics* / Q000502:physiology; D002874:Chromosome Mapping; D003433:Crosses, Genetic; D018744:DNA, Plant / Q000235:genetics; D005638:Fruit / Q000737:chemistry / Q000502:physiology*; D005819:Genetic Markers; D000069600:Plant Breeding; D040641:Quantitative Trait Loci; D059467:Transcriptome; D014867:Water / Q000502:physiology*; D014885:Waxes / Q000737:chemistry
chemical_list: D018744:DNA, Plant; D005819:Genetic Markers; D014885:Waxes; D014867:Water