title: PTENp1, a natural sponge of miR-21, mediates PTEN expression to inhibit the proliferation of oral squamous cell carcinoma.
abstract: PTENp1, non-coding RNA (ncRNA) pseudogene, is involved in oral squamous cell carcinoma (OSCC). The precise effects mediated by PTENp1 transcripts within intricate regulatory networks involving molecular interactions with ancestral gene PTEN and tumorigenicity in OSCC remain unclear. Here, we found that PTENp1 was aberrantly expressed in OSCC. There was a positive correlation between the expression levels of PTENp1 and PTEN. Further, we showed that PTENp1 acted as a competing endogenous RNA that protects PTEN transcripts from being inhibited by miR-21, and consequently inhibited proliferation and colony formation and triggered S-G2/M cell cycle arrest through the AKT pathway. Also, the homogeneous relationship between expression of PTENp1 and PTEN was confirmed in OSCC tumor xenografts. Finally, low expression of PTENp1 and PTEN was negatively associated with histological differentiation and OSCC prognosis. The present work provided the first evidence for the extraordinary crosstalk among PTENp1, PTEN, and miR-21, and rendered a new light on the treatment of OSCC. © 2016 Wiley Periodicals, Inc.
authors.affiliation: Department of Oral Maxillofacial Surgery, The Affiliated Hospital of Qingdao University, Qingdao, Shandong, P. R. China.|Department of Oral Maxillofacial Surgery, Stomatological Hospital, College of Medicine, Xi'an Jiaotong University, Xi'an, Shaanxi, P. R. China.
authors.forename: Ling
authors.initials: L
authors.lastname: Gao
authors.affiliation: Department of Oral Maxillofacial Surgery, The Affiliated Hospital of Qingdao University, Qingdao, Shandong, P. R. China.|Department of Oral Maxillofacial Surgery, Stomatological Hospital, College of Medicine, Xi'an Jiaotong University, Xi'an, Shaanxi, P. R. China.
authors.forename: Wenhao
authors.initials: W
authors.lastname: Ren
authors.affiliation: Department of Oral Maxillofacial Surgery, Stomatological Hospital, College of Medicine, Xi'an Jiaotong University, Xi'an, Shaanxi, P. R. China.
authors.forename: Linmei
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Department of Oral Maxillofacial Surgery, The Affiliated Hospital of Qingdao University, Qingdao, Shandong, P. R. China.
authors.forename: Shaoming
authors.initials: S
authors.lastname: Li
authors.affiliation: Department of Gastroenterology, The Affiliated Hospital of Qingdao University, Qingdao, Shandong, P. R. China.
authors.forename: Xinjuan
authors.initials: X
authors.lastname: Kong
authors.affiliation: Department of Oral Maxillofacial Surgery, Stomatological Hospital, College of Medicine, Xi'an Jiaotong University, Xi'an, Shaanxi, P. R. China.
authors.forename: Hao
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Department of Oral Maxillofacial Surgery, Stomatological Hospital, College of Medicine, Xi'an Jiaotong University, Xi'an, Shaanxi, P. R. China.
authors.forename: Jianwei
authors.initials: J
authors.lastname: Dong
authors.affiliation: Department of Oral Maxillofacial Surgery, Stomatological Hospital, College of Medicine, Xi'an Jiaotong University, Xi'an, Shaanxi, P. R. China.
authors.forename: Guangfeng
authors.initials: G
authors.lastname: Cai
authors.affiliation: Department of Oral Maxillofacial Surgery, Stomatological Hospital, College of Medicine, Xi'an Jiaotong University, Xi'an, Shaanxi, P. R. China.
authors.forename: Changxiong
authors.initials: C
authors.lastname: Jin
authors.affiliation: Department of Oral Maxillofacial Surgery, Stomatological Hospital, College of Medicine, Xi'an Jiaotong University, Xi'an, Shaanxi, P. R. China.
authors.forename: Danqing
authors.initials: D
authors.lastname: Zheng
authors.affiliation: Department of Oral Maxillofacial Surgery, The Affiliated Hospital of Qingdao University, Qingdao, Shandong, P. R. China.|Department of Oral Maxillofacial Surgery, Stomatological Hospital, College of Medicine, Xi'an Jiaotong University, Xi'an, Shaanxi, P. R. China.
authors.forename: Keqian
authors.initials: K
authors.lastname: Zhi
mesh_terms: D000818:Animals; D002294:Carcinoma, Squamous Cell / Q000175:diagnosis / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D002453:Cell Cycle; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D008807:Mice, Inbred BALB C; D035683:MicroRNAs / Q000235:genetics*; D008875:Middle Aged; D009055:Mouth / Q000378:metabolism / Q000473:pathology; D009062:Mouth Neoplasms / Q000175:diagnosis / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D051059:PTEN Phosphohydrolase / Q000235:genetics*; D011379:Prognosis; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism; D011544:Pseudogenes / Q000235:genetics*; D022661:RNA, Untranslated / Q000235:genetics*; D015398:Signal Transduction
chemical_list: C524940:MIRN21 microRNA, human; D035683:MicroRNAs; D022661:RNA, Untranslated; D051057:Proto-Oncogene Proteins c-akt; D051059:PTEN Phosphohydrolase; C494929:PTEN protein, human
keywords: OSCC; PTEN; PTENp1; miR-21; proliferation