title: Composite cell sheet for periodontal regeneration: crosstalk between different types of MSCs in cell sheet facilitates complex periodontal-like tissue regeneration.
abstract: Tissue-engineering strategies based on mesenchymal stem cells (MSCs) and cell sheets have been widely used for periodontal tissue regeneration. However, given the complexity in periodontal structure, the regeneration methods using a single species of MSC could not fulfill the requirement for periodontal regeneration.
  
  
  
  We researched the interaction between the periodontal ligament stem cells (PDLSCs) and jaw bone marrow-derived mesenchymal stem cells (JBMMSCs), and constructed a composite cell sheet comprising both of the above MSCs to regenerate complex periodontium-like structures in nude mice.
  
  
  
  Our results show that by co-culturing PDLSCs and JBMMSCs, the expressions of bone and extracellular matrix (ECM)-related genes and proteins were significantly improved in both MSCs. Further investigations showed that, compared to the cell sheet using PDLSCs or JBMMSCs, the composite stem cell sheet (CSCS), which comprises these two MSCs, expressed higher levels of bone- and ECM-related genes and proteins, and generated a composite structure more similar to the native periodontal tissue physiologically in vivo.
  
  
  
  In conclusion, our results demonstrate that the crosstalk between PDLSCs and JBMMSCs in cell sheets facilitate regeneration of complex periodontium-like structures, providing a promising new strategy for physiological and functional regeneration of periodontal tissue.
authors.affiliation: State Key Laboratory of Military Stomatology, National Clinical Research Center for Oral Diseases, Center for Tissue Engineering, School of Stomatology, The Fourth Military Medical University, Xi'an, Shaanxi, 710032, People's Republic of China.|State Key Laboratory of Military Stomatology, National Clinical Research Center for Oral Diseases, Shanxxi Clinical Research Center for Oral Diseases, Department of Orthodontics, School of Stomatology, The Fourth Military Medical University, Xi'an, Shaanxi, 710032, People's Republic of China.|Research and Development Center for Tissue Engineering, Fourth Military Medical University, Xi'an, Shaanxi, 710032, People's Republic of China.
authors.forename: Hao
authors.initials: H
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Military Stomatology, National Clinical Research Center for Oral Diseases, Center for Tissue Engineering, School of Stomatology, The Fourth Military Medical University, Xi'an, Shaanxi, 710032, People's Republic of China.|Research and Development Center for Tissue Engineering, Fourth Military Medical University, Xi'an, Shaanxi, 710032, People's Republic of China.
authors.forename: Shiyu
authors.initials: S
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Military Stomatology, National Clinical Research Center for Oral Diseases, Center for Tissue Engineering, School of Stomatology, The Fourth Military Medical University, Xi'an, Shaanxi, 710032, People's Republic of China.|Research and Development Center for Tissue Engineering, Fourth Military Medical University, Xi'an, Shaanxi, 710032, People's Republic of China.|Department of Stomatology, General Hospital of Tibet Military Region, Lhasa, Tibet, 850007, People's Republic of China.
authors.forename: Bin
authors.initials: B
authors.lastname: Zhu
authors.affiliation: State Key Laboratory of Military Stomatology, National Clinical Research Center for Oral Diseases, Center for Tissue Engineering, School of Stomatology, The Fourth Military Medical University, Xi'an, Shaanxi, 710032, People's Republic of China.|Research and Development Center for Tissue Engineering, Fourth Military Medical University, Xi'an, Shaanxi, 710032, People's Republic of China.|School and Hospital of Stomatology, Wenzhou Medical University, Wenzhou, Zhejiang, 325003, People's Republic of China.
authors.forename: Qiu
authors.initials: Q
authors.lastname: Xu
authors.affiliation: State Key Laboratory of Military Stomatology, National Clinical Research Center for Oral Diseases, Shanxxi Clinical Research Center for Oral Diseases, Department of Orthodontics, School of Stomatology, The Fourth Military Medical University, Xi'an, Shaanxi, 710032, People's Republic of China. dingyin@fmmu.edu.cn.
authors.forename: Yin
authors.initials: Y
authors.lastname: Ding
authors.affiliation: State Key Laboratory of Military Stomatology, National Clinical Research Center for Oral Diseases, Center for Tissue Engineering, School of Stomatology, The Fourth Military Medical University, Xi'an, Shaanxi, 710032, People's Republic of China. yanjin@fmmu.edu.cn.|Research and Development Center for Tissue Engineering, Fourth Military Medical University, Xi'an, Shaanxi, 710032, People's Republic of China. yanjin@fmmu.edu.cn.
authors.forename: Yan
authors.initials: Y
authors.lastname: Jin
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology; D002454:Cell Differentiation / Q000502:physiology; D018920:Coculture Techniques / Q000379:methods; D005109:Extracellular Matrix / Q000502:physiology; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D010012:Osteogenesis / Q000502:physiology*; D010513:Periodontal Ligament / Q000166:cytology*; D012038:Regeneration / Q000502:physiology*; D033581:Stem Cell Transplantation / Q000379:methods; D023822:Tissue Engineering / Q000379:methods; D014945:Wound Healing / Q000502:physiology
keywords: Bone marrow mesenchymal stem cells; Cell sheet; Periodontal ligament stem cells; Periodontal regeneration; Tissue engineering