title: The role of three heat shock protein genes in the immune response toAeromonas hydrophilachallenge in marbled eel,Anguilla marmorata.
abstract: Heat shock proteins (HSPs) are highly conserved molecular chaperones that play critical roles in both innate and adaptive immunity. However, little information about HSPs from marbled eelAnguilla marmoratais known. In this study, the full-lengthAmhsp90(2527 bp),Amhsp70(2443 bp) andAmhsc70(2247 bp) were first cloned fromA. marmorata, using rapid amplification of cDNA ends, containing open reading frames of 2181, 1932 and 1950 bp in length, and encoding proteins with 726, 643 and 649 amino acids, respectively. The deduced amino acid sequences of threeAmhspsshared a high homology similarity with other migratory fish. Real-time fluorescent quantitative polymerase chain reaction was used to evaluate tissue-specific distribution and mRNA expression levels of threeAmhspssubjected to infection withAeromonas hydrophila. The mRNA expression of threeAmhspsin eight tested tissues, namely liver, heart, muscle, gill, spleen, kidney, brain and intestine, of juvenileA. marmoratawas evaluated to reveal the major expression distribution in liver, intestine, muscle and heart. After pathogen challenge treatments, mRNA transcriptions of threeAmhspsrevealed a significant regulation at various time points in the same tissue. All these findings suggest thatAmhspsmay be involved in the immune response inA. marmorata.
authors.affiliation: Jiangsu Key Laboratory for Biodiversity and Biotechnology, College of Life Sciences, Nanjing Normal University, Nanjing, Jiangsu 210023, People's Republic of China; Co-Innovation Center for Marine Bio-Industry Technology of Jiangsu Province, Lianyungang, Jiangsu 222005, People's Republic of China.
authors.forename: Fenfei
authors.initials: F
authors.lastname: Liang
authors.affiliation: Jiangsu Key Laboratory for Biodiversity and Biotechnology, College of Life Sciences, Nanjing Normal University, Nanjing, Jiangsu 210023, People's Republic of China; Co-Innovation Center for Marine Bio-Industry Technology of Jiangsu Province, Lianyungang, Jiangsu 222005, People's Republic of China.
authors.forename: Guosong
authors.initials: G
authors.lastname: Zhang
authors.affiliation: Jiangsu Key Laboratory for Biodiversity and Biotechnology, College of Life Sciences, Nanjing Normal University, Nanjing, Jiangsu 210023, People's Republic of China; Co-Innovation Center for Marine Bio-Industry Technology of Jiangsu Province, Lianyungang, Jiangsu 222005, People's Republic of China.
authors.forename: Shaowu
authors.identifier: 0000-0003-0802-0930
authors.initials: S
authors.lastname: Yin
authors.affiliation: Jiangsu Key Laboratory for Biodiversity and Biotechnology, College of Life Sciences, Nanjing Normal University, Nanjing, Jiangsu 210023, People's Republic of China; Co-Innovation Center for Marine Bio-Industry Technology of Jiangsu Province, Lianyungang, Jiangsu 222005, People's Republic of China.
authors.forename: Li
authors.initials: L
authors.lastname: Wang
keywords: Aeromonas hydrophila; Anguilla marmorata; heat shock protein; immune response; mRNA expression