title: In vitro anticancer activity of silver nanoparticle synthesized by Escherichia coli VM1 isolated from marine sediments of Ennore southeast coast of India.
abstract: In the present investigation, the bacterium Escherichia coli (VM1) was isolated from a marine sediments of Ennore coastal water along southeast coast of India was exploited for its capability of Ag NPs synthesis. The derived nanoparticles were characterized employing ultraviolet-visible (UV-vis) spectroscopy, Fourier Transform Infrared (FTIR) spectroscopy, X-ray diffraction and High Resolution Transmission Electron Microscopy (HRTEM) studies. The Ag NPs were further subjected to MTT assay to evaluate their anticancer activity against human lung cancer cell line (A549), human cervical cancer cell line (HeLa) and normal (Vero) cell line. The marine E. coli (VM1) synthesized silver nanoparticles exhibited considerable cytotoxic influence against human cancer cell lines, thereby suggesting that they have tremendous potential as effective anticancer agents.
authors.affiliation: Centre of Advance Study in Marine Biology, Faculty of Marine Sciences, Annamalai University, Parangipettai 608 502, India.
authors.forename: Viswanathan
authors.initials: V
authors.lastname: Maharani
authors.affiliation: Centre of Advance Study in Marine Biology, Faculty of Marine Sciences, Annamalai University, Parangipettai 608 502, India. Electronic address: fish_lar@yahoo.com.
authors.forename: Arumugam
authors.initials: A
authors.lastname: Sundaramanickam
authors.affiliation: Centre of Advance Study in Marine Biology, Faculty of Marine Sciences, Annamalai University, Parangipettai 608 502, India.
authors.forename: Thangavel
authors.initials: T
authors.lastname: Balasubramanian
mesh_terms: D000072283:A549 Cells; D000818:Animals; D000970:Antineoplastic Agents / Q000737:chemistry* / Q000494:pharmacology*; D002522:Chlorocebus aethiops; D004354:Drug Screening Assays, Antitumor; D004926:Escherichia coli / Q000145:classification / Q000302:isolation & purification / Q000378:metabolism*; D019015:Geologic Sediments / Q000382:microbiology; D055772:Green Chemistry Technology; D006367:HeLa Cells; D006801:Humans; D066298:In Vitro Techniques; D007194:India; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D012834:Silver / Q000737:chemistry* / Q000494:pharmacology*; D017550:Spectroscopy, Fourier Transform Infrared; D014709:Vero Cells
chemical_list: D000970:Antineoplastic Agents; D012834:Silver
keywords: Anticancer activity; Biosynthesis; Escherichia coli; Silver nanoparticle