title: Lactobacillus salivarius Isolated from Patients with Rheumatoid Arthritis Suppresses Collagen-Induced Arthritis and Increases Treg Frequency in Mice.
abstract: Previously, we demonstrated that Lactobacillus salivarius was more abundant in patients with rheumatoid arthritis (RA), an inflammatory autoimmune disease wherein the gut microbiota is altered, than in healthy individuals. However, the effect of L. salivarius in RA is unclear. Hence, we investigated the effect of L. salivarius isolated from patients with RA on collagen-induced arthritis (CIA) in mice. L. salivarius UCC118 or L. plantarum WCFS1 isolated from patients with RA was administered orally for 5 weeks, starting from 2 weeks before the induction of arthritis in DBA/1 mice. Clinical score progression, histological changes, serum cytokine concentrations, and the proportion of interleukin (IL)-17-producing T cells [T helper 17 (Th17)] and regulatory T cells (Tregs) in the spleen were evaluated. Bone erosion was evaluated by micro-computed tomography. CIA mice treated with either L. salivarius or L. plantarum showed lower arthritis scores, milder synovial infiltration, and less bone erosion when compared with phosphate-buffered, saline-treated CIA mice. Administration of L. salivarius and L. plantarum reduced the Th17 cell fraction and increased the Treg fraction. L. salivarius-treated CIA mice displayed a significant increase in serum anti-inflammatory IL-10 levels. Thus, pretreatment with L. salivarius could significantly improve CIA in mice and may help alleviate RA in a clinical setting.
authors.affiliation: 1 Department of Rheumatology, Southwest Hospital, Third Military Medical University , Chongqing, China .
authors.forename: Xiaofei
authors.initials: X
authors.lastname: Liu
authors.affiliation: 2 Department of Infectious Diseases, Chongqing Key Laboratory of Infectious Diseases, Southwest Hospital, Third Military Medical University , Chongqing, China .
authors.forename: Juan
authors.initials: J
authors.lastname: Zhang
authors.affiliation: 1 Department of Rheumatology, Southwest Hospital, Third Military Medical University , Chongqing, China .
authors.forename: Qinghua
authors.initials: Q
authors.lastname: Zou
authors.affiliation: 1 Department of Rheumatology, Southwest Hospital, Third Military Medical University , Chongqing, China .
authors.forename: Bing
authors.initials: B
authors.lastname: Zhong
authors.affiliation: 1 Department of Rheumatology, Southwest Hospital, Third Military Medical University , Chongqing, China .
authors.forename: Heng
authors.initials: H
authors.lastname: Wang
authors.affiliation: 1 Department of Rheumatology, Southwest Hospital, Third Military Medical University , Chongqing, China .
authors.forename: Fangxiang
authors.initials: F
authors.lastname: Mou
authors.affiliation: 2 Department of Infectious Diseases, Chongqing Key Laboratory of Infectious Diseases, Southwest Hospital, Third Military Medical University , Chongqing, China .
authors.forename: Like
authors.initials: L
authors.lastname: Wu
authors.affiliation: 1 Department of Rheumatology, Southwest Hospital, Third Military Medical University , Chongqing, China .
authors.forename: Yongfei
authors.initials: Y
authors.lastname: Fang
mesh_terms: D000818:Animals; D001169:Arthritis, Experimental; D001172:Arthritis, Rheumatoid / Q000097:blood* / Q000175:diagnosis / Q000276:immunology* / Q000628:therapy; D001706:Biopsy; D016207:Cytokines / Q000097:blood; D004195:Disease Models, Animal; D004797:Enzyme-Linked Immunosorbent Assay; D005434:Flow Cytometry; D006801:Humans; D018836:Inflammation Mediators; D000069996:Ligilactobacillus salivarius / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification; D018655:Lymphocyte Count; D051379:Mice; D050378:T-Lymphocytes, Regulatory / Q000276:immunology* / Q000378:metabolism; D058504:Th17 Cells / Q000276:immunology / Q000378:metabolism; D055114:X-Ray Microtomography
chemical_list: D016207:Cytokines; D018836:Inflammation Mediators
keywords: Lactobacillus salivarius; Th17 cells; collagen-induced arthritis; regulatory T cell; rheumatoid arthritis