title: Cell-type specific variation in the induction of ER stress and downstream events in chikungunya virus infection.
abstract: Over the last decade infections with the mosquito transmitted chikungunya virus (CHIKV) have become a major worldwide concern, and considerable efforts have been made in understanding the interaction of this virus with the host cell machinery. Studies have documented the induction of the unfolded protein response (UPR), as well as the induction of apoptosis and autophagy in response to CHIKV infection. This study comparatively analysed these three processes in two cell lines, Hela and HepG2. Infection of Hela cells was characterized by activation of the PERK/eIF2α branch of the UPR, the induction of autophagy and early apoptosis, while infection of HepG2 cells was characterized by activation of the IRE/XBP1 branch of the UPR, limited or no activation of autophagy and comparatively later apoptosis. These results show that the specific cell context is an important mediator of the host cell response to CHIKV infection.
authors.affiliation: Institute of Molecular Biosciences, Mahidol University, Bangkok, Nakhon Pathom, Thailand.
authors.forename: Sarawut
authors.initials: S
authors.lastname: Khongwichit
authors.affiliation: Institute of Molecular Biosciences, Mahidol University, Bangkok, Nakhon Pathom, Thailand.
authors.forename: Nitwara
authors.initials: N
authors.lastname: Wikan
authors.affiliation: Institute of Molecular Biosciences, Mahidol University, Bangkok, Nakhon Pathom, Thailand.
authors.forename: Bizunesh
authors.initials: B
authors.lastname: Abere
authors.affiliation: Institute of Molecular Biosciences, Mahidol University, Bangkok, Nakhon Pathom, Thailand.
authors.forename: Chutima
authors.initials: C
authors.lastname: Thepparit
authors.affiliation: Institute of Molecular Biosciences, Mahidol University, Bangkok, Nakhon Pathom, Thailand.
authors.forename: Atichat
authors.initials: A
authors.lastname: Kuadkitkan
authors.affiliation: Department of Microbiology, Faculty of Science, Mahidol University, Bangkok, Thailand; Center for Emerging and Neglected Infectious Diseases, Mahidol University, Bangkok, Nakhon Pathom, Thailand.
authors.forename: Sukathida
authors.initials: S
authors.lastname: Ubol
authors.affiliation: Institute of Molecular Biosciences, Mahidol University, Bangkok, Nakhon Pathom, Thailand; Center for Emerging and Neglected Infectious Diseases, Mahidol University, Bangkok, Nakhon Pathom, Thailand. Electronic address: duncan.smi@mahidol.ac.th.
authors.forename: Duncan R
authors.initials: DR
authors.lastname: Smith
mesh_terms: D017209:Apoptosis; D001343:Autophagy; D002646:Chikungunya virus / Q000472:pathogenicity*; D059865:Endoplasmic Reticulum Stress; D004847:Epithelial Cells / Q000502:physiology / Q000821:virology; D006367:HeLa Cells; D056945:Hep G2 Cells; D022781:Hepatocytes / Q000502:physiology / Q000821:virology; D054884:Host-Pathogen Interactions; D006801:Humans; D056811:Unfolded Protein Response
keywords: Apoptosis; Autophagy; Chikungunya; Unfolded protein response