title: IgG4 anti-neurofascin155 antibodies in chronic inflammatory demyelinating polyradiculoneuropathy: Clinical significance and diagnostic utility of a conventional assay.
abstract: We aimed to validate the diagnostic utility of enzyme-linked immunosorbent assay (ELISA) for the detection of anti-neurofascin (NF) 155 antibody in 191 patients with chronic inflammatory demyelinating polyneuropathy (CIDP). Human NF155-based ELISA clearly distinguished between anti-NF155 antibody-positive and -negative sera. Fifteen CIDP patients (8%) were IgG4 anti-human NF155 antibody-positive, which were confirmed by western blot, cell-based assay and immunohistochemical study. None of disease controls or healthy subjects had positive results. Clinical presentation of IgG4 anti-NF155 antibody-positive patients was consistent with those in previous reports. This ELISA combined with determination of the IgG4 subclass is useful in screening for anti-NF155 antibodies.
authors.affiliation: Department of Neurology, Anti-aging and Vascular Medicine, National Defense Medical College, Tokorozawa, Saitama 359-8513, Japan.
authors.forename: Masato
authors.initials: M
authors.lastname: Kadoya
authors.affiliation: Department of Neurology, Anti-aging and Vascular Medicine, National Defense Medical College, Tokorozawa, Saitama 359-8513, Japan. Electronic address: adiak901@ndmc.ac.jp.
authors.forename: Kenichi
authors.initials: K
authors.lastname: Kaida
authors.affiliation: Department of Neurology, Nagoya University Graduate School of Medicine, Nagoya, Aichi 466-8550, Japan.
authors.forename: Haruki
authors.initials: H
authors.lastname: Koike
authors.affiliation: Department of Neurology, Anti-aging and Vascular Medicine, National Defense Medical College, Tokorozawa, Saitama 359-8513, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Takazaki
authors.affiliation: Department of Neurology, Neurological Institute, Graduate School of Medical Sciences, Kyushu University, Higashi-Ku, Fukuoka 812-8582, Japan.
authors.forename: Hidenori
authors.initials: H
authors.lastname: Ogata
authors.affiliation: Department of Neurology, Anti-aging and Vascular Medicine, National Defense Medical College, Tokorozawa, Saitama 359-8513, Japan.
authors.forename: Kota
authors.initials: K
authors.lastname: Moriguchi
authors.affiliation: Department of Neurology, University of Tokyo, Bunkyo-Ku, Tokyo 113-8655, Japan.
authors.forename: Jun
authors.initials: J
authors.lastname: Shimizu
authors.affiliation: Department of Neurology, Tokai University School of Medicine, Isehara, Kanagawa 259-1193, Japan.
authors.forename: Eiichiro
authors.initials: E
authors.lastname: Nagata
authors.affiliation: Department of Neurology, Tokai University School of Medicine, Isehara, Kanagawa 259-1193, Japan.
authors.forename: Shunya
authors.initials: S
authors.lastname: Takizawa
authors.affiliation: Department of Neurology, Kyorin University, Mitaka, Tokyo 181-8611, Japan.
authors.forename: Atsuro
authors.initials: A
authors.lastname: Chiba
authors.affiliation: Department of Neurology, Neurological Institute, Graduate School of Medical Sciences, Kyushu University, Higashi-Ku, Fukuoka 812-8582, Japan.
authors.forename: Ryo
authors.initials: R
authors.lastname: Yamasaki
authors.affiliation: Department of Neurology, Neurological Institute, Graduate School of Medical Sciences, Kyushu University, Higashi-Ku, Fukuoka 812-8582, Japan.
authors.forename: Jun-Ichi
authors.initials: JI
authors.lastname: Kira
authors.affiliation: Department of Neurology, Nagoya University Graduate School of Medicine, Nagoya, Aichi 466-8550, Japan.
authors.forename: Gen
authors.initials: G
authors.lastname: Sobue
authors.affiliation: Department of Neurology, Anti-aging and Vascular Medicine, National Defense Medical College, Tokorozawa, Saitama 359-8513, Japan.
authors.forename: Katsunori
authors.initials: K
authors.lastname: Ikewaki
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000818:Animals; D015815:Cell Adhesion Molecules / Q000235:genetics / Q000276:immunology*; D015816:Cell Adhesion Molecules, Neuronal / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005434:Flow Cytometry; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D057809:HEK293 Cells; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood*; D008297:Male; D008875:Middle Aged; D009414:Nerve Growth Factors / Q000235:genetics / Q000276:immunology*; D020277:Polyradiculoneuropathy, Chronic Inflammatory Demyelinating / Q000097:blood* / Q000276:immunology / Q000473:pathology; D051381:Rats; D012584:Sciatic Nerve / Q000378:metabolism; D018709:Statistics, Nonparametric; D014162:Transfection; D055815:Young Adult
chemical_list: C491323:CNTNAP1 protein, human; D015815:Cell Adhesion Molecules; D015816:Cell Adhesion Molecules, Neuronal; D007074:Immunoglobulin G; C054305:NFASC protein, human; D009414:Nerve Growth Factors; D049452:Green Fluorescent Proteins
keywords: Antibody; Chronic inflammatory demyelinating polyradiculoneuropathy; Enzyme-linked immunosorbent assay; IgG4; Neurofascin155