title: Design of chondroitin sulfate-based polyelectrolyte nanoplexes: Formation of nanocarriers with chitosan and a case study of salmon calcitonin.
abstract: The aim of this work was to examine the formation and properties of chondroitin sulfate (CHON)-based nanoparticles (NPs), namely CHON/chitosan (CHIT), CHON/CHIT/calcitonin (sCT) and CHON/sCT. Both, positively and negatively charged CHON/CHIT NPs have been successfully obtained with properties that were dependent on the polymer mixing ratio, polymer concentration and molecular weight of CHIT. sCT was successfully loaded into CHON/CHIT NPs with efficiency close to 100% and notably high loading (up to 33%). A new type of NPs composed of CHON and sCT (a binary system) has been successfully developed. CHON/sCT NPs offer the advantage of a very high drug loading up to 73%. The particle size of CHON-based NPs increased in PBS, acetate buffer and in HCl solution compared to that in water, but most of them remained in the nano-range even after 24h. The media and composition of the nanocarriers were found to affect the release of sCT.
authors.affiliation: School of Pharmacy and Pharmaceutical Sciences, Trinity College Dublin, Dublin 2, Ireland; INSERM U1066, Micro et Nanomédecines Biomimétiques, Angers, France. Electronic address: umerskam@tcd.ie.
authors.forename: Anita
authors.initials: A
authors.lastname: Umerska
authors.affiliation: School of Pharmacy and Pharmaceutical Sciences, Trinity College Dublin, Dublin 2, Ireland. Electronic address: ocorrign@tcd.ie.
authors.forename: Owen I
authors.initials: OI
authors.lastname: Corrigan
authors.affiliation: School of Pharmacy and Pharmaceutical Sciences, Trinity College Dublin, Dublin 2, Ireland. Electronic address: lidia.tajber@tcd.ie.
authors.forename: Lidia
authors.initials: L
authors.lastname: Tajber
mesh_terms: D002116:Calcitonin / Q000737:chemistry*; D048271:Chitosan / Q000737:chemistry*; D002809:Chondroitin Sulfates / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D053758:Nanoparticles / Q000737:chemistry*; D010316:Particle Size; D000071228:Polyelectrolytes / Q000737:chemistry*
chemical_list: D000071228:Polyelectrolytes; C028815:salmon calcitonin; D002116:Calcitonin; D002809:Chondroitin Sulfates; D048271:Chitosan
keywords: Calcitonin; Chitosan; Chondroitin sulfate; Nanoparticles; Peptide; Polyelectrolyte complexes