title: Immunogenicity of porcine circovirus type 2 nucleic acid vaccine containing CpG motif for mice.
abstract: BACKGROUND
  This study aimed at reseaching the immune effect of porcine circovirus type 2 (PCV2) DNA vaccine containing CpG motif on mice.
  
  
  METHODS
  A total of 40 6-week-old female BALB/c mice were randomly divided into four groups which were immunized by 18CpG-pVAX1-ORF2, pVAX1-ORF2, pVAX1 and PBS, respectively, and immunized again 2 weeks later. All mice were challenged with 0.2 mL PCV2 cells virulent strain SD (106.0TCID50/mL) after 4 weeks. Average daily gain, blood antibody levels, microscopic changes and viremia were detected to estimate the effect of DNA vaccine.
  
  
  CONCLUSIONS
  The results showed that compared to those of the control mice, groups immunized with pVAX1-ORF2 and 18CpG-pVAX1-ORF2 could induce PCV2-specific antibodies. The PCV2-specific antibodies level of 18 CpG-pVAX1-ORF2 groups was higher significantly than other groups and decreased slowly along with time. There was no distinct pathological damage and viremia occurring in mice that inoculated with CpG motif DNA vaccines. The results demonstrated that the DNA vaccine containing 18 CpG could build up resistibility immunity and reduce immune organ damage on mice.
authors.affiliation: Division of Swine Diseases, Shandong Provincial Key Laboratory of Animal Disease Control & Breeding, Institute of Animal Science and Veterinary Medicine Shandong Academy of Agricultural Sciences, Jinan, 250100, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Li
authors.affiliation: Division of Swine Diseases, Shandong Provincial Key Laboratory of Animal Disease Control & Breeding, Institute of Animal Science and Veterinary Medicine Shandong Academy of Agricultural Sciences, Jinan, 250100, China.
authors.forename: Jiang
authors.initials: J
authors.lastname: Yu
authors.affiliation: Division of Swine Diseases, Shandong Provincial Key Laboratory of Animal Disease Control & Breeding, Institute of Animal Science and Veterinary Medicine Shandong Academy of Agricultural Sciences, Jinan, 250100, China.
authors.forename: Shaojian
authors.initials: S
authors.lastname: Xu
authors.affiliation: Division of Swine Diseases, Shandong Provincial Key Laboratory of Animal Disease Control & Breeding, Institute of Animal Science and Veterinary Medicine Shandong Academy of Agricultural Sciences, Jinan, 250100, China.
authors.forename: Jianli
authors.initials: J
authors.lastname: Shi
authors.affiliation: Division of Swine Diseases, Shandong Provincial Key Laboratory of Animal Disease Control & Breeding, Institute of Animal Science and Veterinary Medicine Shandong Academy of Agricultural Sciences, Jinan, 250100, China.
authors.forename: Shengnan
authors.initials: S
authors.lastname: Xu
authors.affiliation: Division of Swine Diseases, Shandong Provincial Key Laboratory of Animal Disease Control & Breeding, Institute of Animal Science and Veterinary Medicine Shandong Academy of Agricultural Sciences, Jinan, 250100, China.
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Wu
authors.affiliation: Division of Swine Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, 150001, China.
authors.forename: Fang
authors.initials: F
authors.lastname: Fu
authors.affiliation: Division of Swine Diseases, Shandong Provincial Key Laboratory of Animal Disease Control & Breeding, Institute of Animal Science and Veterinary Medicine Shandong Academy of Agricultural Sciences, Jinan, 250100, China.
authors.forename: Zhe
authors.initials: Z
authors.lastname: Peng
authors.affiliation: Division of Swine Diseases, Shandong Provincial Key Laboratory of Animal Disease Control & Breeding, Institute of Animal Science and Veterinary Medicine Shandong Academy of Agricultural Sciences, Jinan, 250100, China.
authors.forename: Lingling
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Division of Swine Diseases, Shandong Provincial Key Laboratory of Animal Disease Control & Breeding, Institute of Animal Science and Veterinary Medicine Shandong Academy of Agricultural Sciences, Jinan, 250100, China.
authors.forename: Shuxuan
authors.initials: S
authors.lastname: Zheng
authors.affiliation: Division of Swine Diseases, Shandong Provincial Key Laboratory of Animal Disease Control & Breeding, Institute of Animal Science and Veterinary Medicine Shandong Academy of Agricultural Sciences, Jinan, 250100, China.
authors.forename: Xiaoyuan
authors.initials: X
authors.lastname: Yuan
authors.affiliation: Division of Swine Diseases, Shandong Provincial Key Laboratory of Animal Disease Control & Breeding, Institute of Animal Science and Veterinary Medicine Shandong Academy of Agricultural Sciences, Jinan, 250100, China.
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Cong
authors.affiliation: Division of Swine Diseases, Shandong Provincial Key Laboratory of Animal Disease Control & Breeding, Institute of Animal Science and Veterinary Medicine Shandong Academy of Agricultural Sciences, Jinan, 250100, China.
authors.forename: Wenbo
authors.initials: W
authors.lastname: Sun
authors.affiliation: Division of Swine Diseases, Shandong Provincial Key Laboratory of Animal Disease Control & Breeding, Institute of Animal Science and Veterinary Medicine Shandong Academy of Agricultural Sciences, Jinan, 250100, China.
authors.forename: Kaihui
authors.initials: K
authors.lastname: Cheng
authors.affiliation: Division of Swine Diseases, Shandong Provincial Key Laboratory of Animal Disease Control & Breeding, Institute of Animal Science and Veterinary Medicine Shandong Academy of Agricultural Sciences, Jinan, 250100, China.
authors.forename: Yijun
authors.initials: Y
authors.lastname: Du
authors.affiliation: Division of Swine Diseases, Shandong Provincial Key Laboratory of Animal Disease Control & Breeding, Institute of Animal Science and Veterinary Medicine Shandong Academy of Agricultural Sciences, Jinan, 250100, China.
authors.forename: Jiaqiang
authors.initials: J
authors.lastname: Wu
authors.affiliation: Division of Swine Diseases, Shandong Provincial Key Laboratory of Animal Disease Control & Breeding, Institute of Animal Science and Veterinary Medicine Shandong Academy of Agricultural Sciences, Jinan, 250100, China. wangjb@saas.ac.cn.
authors.forename: Jinbao
authors.initials: J
authors.lastname: Wang
mesh_terms: D000276:Adjuvants, Immunologic; D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D001835:Body Weight; D018173:Circoviridae Infections / Q000473:pathology / Q000517:prevention & control*; D017925:Circovirus / Q000276:immunology*; D018899:CpG Islands; D004195:Disease Models, Animal; D005260:Female; D007115:Immunization Schedule; D008807:Mice, Inbred BALB C; D016896:Treatment Outcome; D019444:Vaccines, DNA / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*; D014765:Viral Vaccines / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*; D014766:Viremia / Q000517:prevention & control
chemical_list: D000276:Adjuvants, Immunologic; D000914:Antibodies, Viral; D019444:Vaccines, DNA; D014765:Viral Vaccines
keywords: CpG motifs; Immunogenicity; Mice; Nucleic acid vaccine; Porcine circovirus type 2