title: Comparison of bacterial community characteristics between complete and shortcut denitrification systems for quinoline degradation.
abstract: For quinoline-denitrifying degradation, very few researches focused on shortcut denitrification process and its bacterial community characteristics. In this study, complete and shortcut denitrification systems were constructed simultaneously for quinoline degradation. By calculation, specific quinoline removal rates were 0.905 and 1.123 g/(gVSS d), respectively, in the complete and shortcut systems, and the latter was 1.24 times of the former. Polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE), high-throughput sequencing, and quantitative PCR (qPCR) techniques based on 16S rRNA were jointly applied to compare microbial community structures of two systems. Many denitrifying bacteria phyla, classes, and genera were detected in the two systems. Phylum Proteobacteria, Class Gammaproteobacteria, and Genus Alicycliphilus denitrificans were the dominant contributors for quinoline-denitrifying degradation. In the shortcut denitrification system, main and specific strains playing crucial roles were more; the species richness and the total abundance of functional genes (narG, nirS, nirK, and nosZ) were higher compared with the complete denitrification system. It could be supposed that inorganic-nitrogen reductase activity of bacterial community was stronger in the shortcut denitrification system, which was the intrinsic reason to result in higher denitrification rate.
authors.affiliation: Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, 266101, China.
authors.forename: Xiaomei
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Department of Scientific Research and Development, Tsinghua University, Beijing, 100084, China. hua_xiufu@163.com.
authors.forename: Xiufu
authors.initials: X
authors.lastname: Hua
authors.affiliation: Environmental Science and Engineering Institute, Taiyuan University of Technology, 79 Yingze West St., Taiyuan, 030024, China.
authors.forename: Xiuping
authors.initials: X
authors.lastname: Yue
mesh_terms: D001419:Bacteria / Q000201:enzymology / Q000235:genetics / Q000378:metabolism*; D058645:Denaturing Gradient Gel Electrophoresis; D058440:Denitrification / Q000235:genetics / Q000502:physiology; D020563:Gammaproteobacteria / Q000201:enzymology / Q000235:genetics / Q000378:metabolism; D020560:Proteobacteria / Q000201:enzymology / Q000235:genetics / Q000378:metabolism; D011804:Quinolines / Q000378:metabolism*; D012336:RNA, Ribosomal, 16S / Q000235:genetics
chemical_list: D011804:Quinolines; D012336:RNA, Ribosomal, 16S
keywords: Bacterial community; High-throughput sequencing; PCR-DGGE; Quinoline degradation; Shortcut denitrification; qPCR