title: Retroviral Transduction of Helper T Cells as a Genetic Approach to Study Mechanisms Controlling their Differentiation and Function.
abstract: Helper T cell development and function must be tightly regulated to induce an appropriate immune response that eliminates specific pathogens yet prevents autoimmunity. Many approaches involving different model organisms have been utilized to understand the mechanisms controlling helper T cell development and function. However, studies using mouse models have proven to be highly informative due to the availability of genetic, cellular, and biochemical systems. One genetic approach in mice used by many labs involves retroviral transduction of primary helper T cells. This is a powerful approach due to its relative ease, making it accessible to almost any laboratory with basic skills in molecular biology and immunology. Therefore, multiple genes in wild type or mutant forms can readily be tested for function in helper T cells to understand their importance and mechanisms of action. We have optimized this approach and describe here the protocols for production of high titer retroviruses, isolation of primary murine helper T cells, and their transduction by retroviruses and differentiation toward the different helper subsets. Finally, the use of this approach is described in uncovering mechanisms utilized by microRNAs (miRNAs) to regulate pathways controlling helper T cell development and function.
authors.affiliation: Department of Comparative Biomedical Sciences, The Royal Veterinary College; Institute of Physiology I, Cardiology & Vascular Medicine, Eberhard Karls University of Tuebingen.
authors.forename: Yogesh
authors.initials: Y
authors.lastname: Singh
authors.affiliation: Department of Clinical Science and Services, The Royal Veterinary College.
authors.forename: Oliver A
authors.initials: OA
authors.lastname: Garden
authors.affiliation: Institute of Physiology I, Cardiology & Vascular Medicine, Eberhard Karls University of Tuebingen.
authors.forename: Florian
authors.initials: F
authors.lastname: Lang
authors.affiliation: Department of Comparative Biomedical Sciences, The Royal Veterinary College; bcobb@rvc.ac.uk.
authors.forename: Bradley S
authors.initials: BS
authors.lastname: Cobb
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D051379:Mice; D035683:MicroRNAs; D012190:Retroviridae; D006377:T-Lymphocytes, Helper-Inducer; D014161:Transduction, Genetic
chemical_list: D035683:MicroRNAs