title: No association betweenGSTM1andGSTT1genetic polymorphisms and susceptibility to opium sap dependence.
abstract: Glutathione S-transferases (GSTs; EC: 2.5.1.18) are a ubiquitous family of eukaryotic and prokaryotic phase II metabolic isozymes. Genes encodingGSTM1(OMIM: 138350), andGSTT1(OMIM: 600436) are members of class mu and theta, respectively. The most common polymorphism in the GSTM1 is a deletion of the whole GSTM1 gene with a lack of enzyme activity. A homozygous deletion in the GSTT1 has also been reported (null genotypes of GSTT1). The aim of the present study was to investigate the association betweenGSTM1andGSTT1polymorphisms and risk of dependency to opium sap. The present study was performed in Shiraz (southern Iran). In total, 71 males dependent to opium sap and 590 healthy males (as a control group) were included in this study. The genotypes ofGSTM1andGSTT1polymorphisms were determined by PCR. Our data indicate that neitherGSTM1(OR=0.78, 95% CI: 0.47-1.27, P=0.325) norGSTT1(OR=1.25, 95% CI: 0.70-2.21, P=0.442) null genotypes significantly associated with the risk of opium sap dependence. There is no additive effect of the null genotypes ofGSTT1andGSTM1in relation to the risk of dependency to opium sap. The present study indicated that the null genotypes ofGSTT1andGSTM1are not risk factor for opium sap dependence.
authors.affiliation: Department of Biology, College of Sciences, Shiraz University, Shiraz 71467-13565, Iran.
authors.forename: Khyber
authors.initials: K
authors.lastname: Saify
authors.affiliation: Department of Biology, College of Sciences, Shiraz University, Shiraz 71467-13565, Iran.
authors.forename: Mohammad Rashid
authors.initials: MR
authors.lastname: Khalighinasab
authors.affiliation: Department of Biology, College of Sciences, Shiraz University, Shiraz 71467-13565, Iran.
authors.forename: Mostafa
authors.initials: M
authors.lastname: Saadat
keywords: GSTM1; GSTT1; Opium sap dependence; Polymorphism