title: Gli1 promotes colorectal cancer metastasis in a Foxm1-dependent manner by activating EMT and PI3K-AKT signaling.
abstract: Colorectal cancer(CRC) is one of the most commonly diagnosed cancers in human beings and metastasis is the main death reason. Recently, Gli1 has been reported to be a key regulator of various cancer biologies and genes expressions. However, the detailed molecular mechanism of Gli1 in CRC metastasis remains largely unknown. In this study, we aimed to investigate the role of Gli1 in CRC metastasis. We used qRT-PCR, Immunohistochemistry and Western blot to test the expression levels of Gli1, Foxm1 and other target genes in the tissues and cells; Lentivirus stable transfection to change the expression levels of Gli1 and Foxm1; Wound-healing, cell invasion, migration assays and tail vein metastatic assay to test the role of Gli1 in CRC metastasis in vitro and vivo. We demonstrated that Gli1 was significantly overexpressed in colorectal cancer tissues and cells. Foxm1 level had a positive correlation with Gli1. Furthermore, we found that Gli1 promotes colorectal cancer cells metastasis in a Foxm1-dependent manner by activating EMT and PI3K-AKT signaling. Thus, we proved that Gli1 plays important role in CRC metastasis and provided a new visual field on the therapy of CRC metastasis.
authors.affiliation: Colorectal Department of the First Affiliated Hospital of Nanjing Medical University , Nanjing, Jiangsu CHINA, 210029.
authors.forename: Chuan
authors.initials: C
authors.lastname: Zhang
authors.affiliation: Colorectal Department of the First Affiliated Hospital of Nanjing Medical University , Nanjing, Jiangsu CHINA, 210029.
authors.forename: Yong
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Colorectal Department of the First Affiliated Hospital of Nanjing Medical University , Nanjing, Jiangsu CHINA, 210029.
authors.forename: YiFei
authors.initials: Y
authors.lastname: Feng
authors.affiliation: Colorectal Department of the First Affiliated Hospital of Nanjing Medical University , Nanjing, Jiangsu CHINA, 210029.
authors.forename: Yue
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Colorectal Department of the First Affiliated Hospital of Nanjing Medical University , Nanjing, Jiangsu CHINA, 210029.
authors.forename: Bing
authors.initials: B
authors.lastname: Ji
authors.affiliation: Colorectal Department of the First Affiliated Hospital of Nanjing Medical University , Nanjing, Jiangsu CHINA, 210029.
authors.forename: Sen
authors.initials: S
authors.lastname: Wang
authors.affiliation: Colorectal Department of the First Affiliated Hospital of Nanjing Medical University , Nanjing, Jiangsu CHINA, 210029.
authors.forename: Ye
authors.initials: Y
authors.lastname: Sun
authors.affiliation: Colorectal Department of the First Affiliated Hospital of Nanjing Medical University , Nanjing, Jiangsu CHINA, 210029.
authors.forename: Chunyan
authors.initials: C
authors.lastname: Zhu
authors.affiliation: Colorectal Department of the First Affiliated Hospital of Nanjing Medical University , Nanjing, Jiangsu CHINA, 210029.
authors.forename: Dongsheng
authors.initials: D
authors.lastname: Zhang
authors.affiliation: Colorectal Department of the First Affiliated Hospital of Nanjing Medical University , Nanjing, Jiangsu CHINA, 210029.
authors.forename: Yueming
authors.initials: Y
authors.lastname: Sun
mesh_terms: D000328:Adult; D000368:Aged; D000818:Animals; D045744:Cell Line, Tumor; D015179:Colorectal Neoplasms / Q000401:mortality / Q000473:pathology*; D058750:Epithelial-Mesenchymal Transition; D066246:ErbB Receptors / Q000502:physiology; D005260:Female; D000072278:Forkhead Box Protein M1 / Q000502:physiology*; D006801:Humans; D008297:Male; D051379:Mice; D008875:Middle Aged; D009362:Neoplasm Metastasis; D019869:Phosphatidylinositol 3-Kinases / Q000502:physiology*; D051057:Proto-Oncogene Proteins c-akt / Q000502:physiology*; D000071676:Zinc Finger Protein GLI1 / Q000502:physiology*
chemical_list: C107585:FOXM1 protein, human; D000072278:Forkhead Box Protein M1; C400551:GLI1 protein, human; D000071676:Zinc Finger Protein GLI1; D019869:Phosphatidylinositol 3-Kinases; D066246:ErbB Receptors; D051057:Proto-Oncogene Proteins c-akt
keywords: Gli1; PI3K-AKT signaling; colorectal cancer; epithelial-to-mesenchymal transition; metastasis