title: FRET-based aptamer biosensor for selective and sensitive detection of aflatoxin B1 in peanut and rice.
abstract: Aflatoxins are potential food pollutants produced by fungi. Among them, Aflatoxin B1 (AFB1) is the most toxic. Therefore, a great deal of concern is associated with AFB1 toxicity. In this work, utilizing a FRET-based method, we have developed a nanobiosensor for detection of AFB1 in agricultural foods. Aptamer-conjugated Quantum dots (QDs) are adsorbed to Au nanoparticles (AuNPs) due to interaction of aptamers with AuNPs leading to quenching effect on QDs fluorescence. Upon the addition of AFB1, the specific aptamers are attracted to AFB1, getting distance from AuNPs which result in fluorescence recovery. Under optimized conditions the detection limit of proposed nanobiosensor was 3.4nM with linear range of 10-400nM. Selectivity test demonstrates that the nanobiosensor could be a promising tool for specific evaluation of food stuff. This method was successfully applied for the analysis of AFB1 in rice and peanut samples.
authors.affiliation: Department of Life Science Engineering, Faculty of New Sciences & Technologies, University of Tehran, Tehran, Iran.
authors.forename: Fereshte Sadat
authors.initials: FS
authors.lastname: Sabet
authors.affiliation: Department of Life Science Engineering, Faculty of New Sciences & Technologies, University of Tehran, Tehran, Iran; Medical Biomaterials Research Center, Tehran University of Medical Sciences, Tehran, Iran. Electronic address: smhosseini@khayam.ut.ac.ir.
authors.forename: Morteza
authors.initials: M
authors.lastname: Hosseini
authors.affiliation: Department of Life Science Engineering, Faculty of New Sciences & Technologies, University of Tehran, Tehran, Iran.
authors.forename: Hossein
authors.initials: H
authors.lastname: Khabbaz
authors.affiliation: Department of Biotechnology, Payame Noor University, Tehran, Iran.
authors.forename: Mehdi
authors.initials: M
authors.lastname: Dadmehr
authors.affiliation: Center of Excellence in Electrochemistry, Faculty of Chemistry, University of Tehran, Tehran, Iran; Biosensor Research Center, Endocrinology & Metabolism Molecular-Cellular Sciences Institute, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Mohammad Reza
authors.initials: MR
authors.lastname: Ganjali
mesh_terms: D016604:Aflatoxin B1 / Q000032:analysis* / Q000378:metabolism; D052157:Aptamers, Nucleotide / Q000378:metabolism*; D010367:Arachis / Q000254:growth & development / Q000378:metabolism*; D015374:Biosensing Techniques / Q000379:methods*; D005453:Fluorescence; D031541:Fluorescence Resonance Energy Transfer / Q000379:methods*; D005504:Food Analysis; D006046:Gold / Q000737:chemistry; D057230:Limit of Detection; D053768:Metal Nanoparticles / Q000737:chemistry; D012275:Oryza / Q000254:growth & development / Q000378:metabolism*; D045663:Quantum Dots
chemical_list: D052157:Aptamers, Nucleotide; D006046:Gold; D016604:Aflatoxin B1
keywords: Aflatoxin B1; Aptasensor; Au nanoparticle; FRET; Fluorescence; Food; Nanobiosensor; Quantum dots; Sensitive detection