title: One-pot four-enzyme synthesis of thymidinediphosphate-l-rhamnose.
abstract: A new, robust one-pot four-enzyme synthetic method was developed for thymidinediphosphate-l-rhamnose starting from d-glucose-1-phosphate. The enzymes, Glc-1-P thymidylyltransferase, dTDP-Glc-4,6-dehydratase, dTDP-4-keto-6-deoxy-Glc-3,5-epimerase and dTDP-4-keto-Rha reductase were derived from Streptococcus pneumonia serotype 23F, expressed in Escherichia coli, and studied in detail to provide the first direct evidence for their functions.
authors.affiliation: National Glycoengineering Research Center and Shandong Provincial Key Laboratory of Carbohydrate Chemistry and Glycobiology, Shandong University, 27 Shanda Nan Lu, Jinan 250100, China. guofenggu@sdu.edu.cn chenzg@sdu.edu.cn.
authors.forename: Siqiang
authors.initials: S
authors.lastname: Li
authors.affiliation: National Glycoengineering Research Center and Shandong Provincial Key Laboratory of Carbohydrate Chemistry and Glycobiology, Shandong University, 27 Shanda Nan Lu, Jinan 250100, China. guofenggu@sdu.edu.cn chenzg@sdu.edu.cn.
authors.forename: Hong
authors.initials: H
authors.lastname: Wang
authors.affiliation: National Glycoengineering Research Center and Shandong Provincial Key Laboratory of Carbohydrate Chemistry and Glycobiology, Shandong University, 27 Shanda Nan Lu, Jinan 250100, China. guofenggu@sdu.edu.cn chenzg@sdu.edu.cn.
authors.forename: Juncai
authors.initials: J
authors.lastname: Ma
authors.affiliation: National Glycoengineering Research Center and Shandong Provincial Key Laboratory of Carbohydrate Chemistry and Glycobiology, Shandong University, 27 Shanda Nan Lu, Jinan 250100, China. guofenggu@sdu.edu.cn chenzg@sdu.edu.cn.
authors.forename: Guofeng
authors.initials: G
authors.lastname: Gu
authors.affiliation: National Glycoengineering Research Center and Shandong Provincial Key Laboratory of Carbohydrate Chemistry and Glycobiology, Shandong University, 27 Shanda Nan Lu, Jinan 250100, China. guofenggu@sdu.edu.cn chenzg@sdu.edu.cn.
authors.forename: Zonggang
authors.initials: Z
authors.lastname: Chen
authors.affiliation: Department of Chemistry, University of Florida, 214 Leigh Hall, Gainesville, Florida 32611, USA. zguo@chem.ufl.edu.
authors.forename: Zhongwu
authors.initials: Z
authors.lastname: Guo