title: A facile and green strategy for the synthesis of Au, Ag and Au-Ag alloy nanoparticles using aerial parts of R. hypocrateriformis extract and their biological evaluation.
abstract: A facile and green strategy is reported here to synthesize gold (Au), silver (Ag) and gold-silver (Au-Ag) alloy nanoparticles (NPs) through bio-reduction reactions of aqueous corresponding metal precursors mediated by extracts of aerial parts of R. hypocrateriformis, which act as both reducing and stabilizing agents, under microwave irradiation. UV-vis spectrophotometer, XRD, FT-IR, FESEM/TEM, TGA and EDAX analysis were used to characterize the obtained NPs. The formation of NPs is evident from their surface plasmon resonance peak observed at λmax=∼550, 450 and 500nm for Au, Ag and Au-Ag alloy NPs respectively. XRD pattern revealed that fcc structure, while FT-IR spectra signify the presence of phytochemicals adsorbed on NPs. Such a biofunctionalized NPs were characterized by their weight loss, 30% due to thermal degradation of plant phytochemicals observed in TG analysis. The spherical shape of Au, Ag and Au-Ag alloy NPs (∼10-50nm) is observed by FE-SEM/TEM images. EDAX analysis confirms the expected elemental composition. Moreover, these NPs showed enhanced antimicrobial, antioxidant, and anticancer activities, though it is more pronounced for Au-Ag alloy NPs, which is due to the combining effect of phytochemicals, Au and Ag metals. Thus, the biosynthesized NPs could be applied as effective growth inhibitors for various biomedical applications.
authors.affiliation: Central Research Laboratory, Department of Studies and Research in Chemistry, Gulbarga University, Gulbarga, 585 106, India.
authors.forename: S S
authors.initials: SS
authors.lastname: Godipurge
authors.affiliation: BMS R&D Centre, BMS College of Engineering, Bangalore, 560019, India.
authors.forename: S
authors.initials: S
authors.lastname: Yallappa
authors.affiliation: Nargund College Of Pharmacy, Bangalore, 560085, India.
authors.forename: Naveen J
authors.initials: NJ
authors.lastname: Biradar
authors.affiliation: Central Research Laboratory, Department of Studies and Research in Chemistry, Gulbarga University, Gulbarga, 585 106, India. Electronic address: jsbiradar55@gmail.com.
authors.forename: J S
authors.initials: JS
authors.lastname: Biradar
authors.affiliation: Toxicology and Drug Discovery Centre for Emerging Technologies, Jain University, Ramanagara, 562 112, India.
authors.forename: B L
authors.initials: BL
authors.lastname: Dhananjaya
authors.affiliation: Environmental Management and Policy Research Centre, Bangalore, 560018, India.
authors.forename: Gajanan
authors.initials: G
authors.lastname: Hegde
authors.affiliation: Center for Materials Science and Technology, Vijnana Bhavan, University of Mysore, Manasagangothri, Mysuru, 570006, India.
authors.forename: K
authors.initials: K
authors.lastname: Jagadish
authors.affiliation: BMS R&D Centre, BMS College of Engineering, Bangalore, 560019, India.
authors.forename: Gurumurthy
authors.initials: G
authors.lastname: Hegde
mesh_terms: D000497:Alloys / Q000737:chemistry / Q000494:pharmacology; D000818:Animals; D000890:Anti-Infective Agents / Q000737:chemistry / Q000494:pharmacology; D000970:Antineoplastic Agents / Q000737:chemistry / Q000494:pharmacology; D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology; D001709:Biotechnology; D045744:Cell Line, Tumor; D002522:Chlorocebus aethiops; D027722:Convolvulaceae / Q000378:metabolism*; D006046:Gold / Q000737:chemistry* / Q000494:pharmacology; D006047:Gold Alloys / Q000737:chemistry / Q000494:pharmacology; D055772:Green Chemistry Technology; D006801:Humans; D061986:MCF-7 Cells; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D036103:Nanotechnology; D010936:Plant Extracts / Q000378:metabolism; D012834:Silver / Q000737:chemistry* / Q000494:pharmacology; D014709:Vero Cells
chemical_list: D000497:Alloys; D000890:Anti-Infective Agents; D000970:Antineoplastic Agents; D000975:Antioxidants; D006047:Gold Alloys; D010936:Plant Extracts; D012834:Silver; D006046:Gold
keywords: Anticancer activity; Antimicrobial activity; Antioxidant activity; Gold-silver alloys; R. hypocrateriformis