title: Isolation of lactic acid bacteria bound to the porcine intestinal mucosa and an analysis of their moonlighting adhesins.
abstract: The adhesion of lactic acid bacteria (LAB) to the intestinal mucosa is one of the criteria in selecting for probiotics. Eighteen LAB were isolated from porcine intestinal mucin (PIM): ten strains ofLactobacillus, six strains ofWeissella, and two strains ofStreptococcus. Using sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) for phosphate-buffered saline (PBS) extracts from the LAB, many bands were detected in half of the samples, while a few and/or no clear bands were detected in the other half. All six of the selected LAB showed adhesion to PIM.L. johnsoniiMYU 214 and MYU 221 showed adhesion at more than 10%.W. viridescensMYU 208,L. reuteriMYU 213,L. mucosaeMYU 225, andL. agilisMYU 227 showed medium levels of adhesion at 5.9-8.3%. In a comprehensive analysis for the adhesins in the PBS extracts using a receptor overlay analysis, many moonlighting proteins were detected and identified as candidates for adhesins: GroEL, enolase, and elongation factor Tu in MYU 208; peptidase C1, enolase, formyl-CoA transferase, phosphoglyceromutase, triosephosphate isomerase, and phosphofructokinase in MYU 221; and DnaK, enolase, and phosphoglycerate kinase in MYU 227. These proteins in the PBS extracts, which included such things as molecular chaperones and glycolytic enzymes, may play important roles as adhesins.
authors.affiliation: Laboratory of Food Biochemistry, Department of Bioscience, School of Agriculture, Tokai University, Kawayo, Minami Aso-mura, Aso-gun, Kumamoto, Japan.
authors.forename: Hideki
authors.initials: H
authors.lastname: Kinoshita
authors.affiliation: Department of Food Management, School of Food, Agricultural and Environmental Sciences, Miyagi University, 2-2-1 Hatatate, Taihaku-ku, Sendai, Miyagi, Japan.
authors.forename: Satoko
authors.initials: S
authors.lastname: Ohuchi
authors.affiliation: Graduate School of Environmental and Life Science, Okayama University, 1-1-1 Tsushima-naka, Okayama, Japan.
authors.forename: Kensuke
authors.initials: K
authors.lastname: Arakawa
authors.affiliation: Research Faculty of Agriculture, Hokkaido University, Kita 9, Nishi 9, Kita-ku, Sapporo, Hokkaido 060-8589, Japan.
authors.forename: Masamichi
authors.initials: M
authors.lastname: Watanabe
authors.affiliation: Laboratory of Animal Products Chemistry, Graduate School of Agricultural Science, Tohoku University, 1-1 Tsutsumidori-Amamiyamachi, Aoba-ku, Sendai, Miyagi, Japan.
authors.forename: Haruki
authors.initials: H
authors.lastname: Kitazawa
authors.affiliation: Laboratory of Animal Products Chemistry, Graduate School of Agricultural Science, Tohoku University, 1-1 Tsutsumidori-Amamiyamachi, Aoba-ku, Sendai, Miyagi, Japan.
authors.forename: Tadao
authors.initials: T
authors.lastname: Saito
keywords: adhesin; adhesion; lactic acid bacteria; moonlighting protein; porcine intestinal mucin; probiotics