title: Grape seed and skin extract reduces pancreas lipotoxicity, oxidative stress and inflammation in high fat diet fed rats.
abstract: Obesity is related to an elevated risk of diabetes and the mechanisms whereby fat adversely affects the pancreas are poorly understood. We studied the effect of a high fat diet (HFD) on pancreas steatosis, oxidative stress and inflammation as well as the putative protection afforded by grape seed and skin extract (GSSE). HFD induced body weight gain, without affecting insulinemia, nor glycemia and dropped adiponectemia. HFD also provoked the ectopic deposition of cholesterol and triglyceride, and an oxidative stress characterized by increased lipoperoxidation and carbonylation, inhibition of antioxidant enzyme activities such as CAT, GPx and SOD, depletion of zinc and a concomitant increase in calcium and H2O2. HFD induced pro-inflammatory chemokines mRNA as RANTES and MCP1 as well as cytokines expression as TNFα, IL6 and IL1β. Importantly GSSE counteracted all the deleterious effects of HFD on pancreas in vivo i-e lipotoxicity, oxidative stress and inflammation. In conclusion, GSSE could find potential applications in fat-induced pancreas lipotoxicity and dysfunction.
authors.affiliation: Université de Carthage, Faculté des Sciences de Bizerte, 7021 Jarzouna, Tunisia; Laboratoire des Substances Bioactives (LSBA), Centre de Biotechnologie de Borj Cedria, BP-901, 2050 Hammam-Lif, Tunisia.
authors.forename: Faten
authors.initials: F
authors.lastname: Aloui
authors.affiliation: Université de Carthage, Faculté des Sciences de Bizerte, 7021 Jarzouna, Tunisia; Laboratoire des Substances Bioactives (LSBA), Centre de Biotechnologie de Borj Cedria, BP-901, 2050 Hammam-Lif, Tunisia. Electronic address: charradi3@yahoo.com.
authors.forename: Kamel
authors.initials: K
authors.lastname: Charradi
authors.affiliation: Physiologie de la Nutrition et Toxicologie, UMR U866 INSERM/Université de Bourgogne/Agro-Sup, 6, Boulevard Gabriel, Dijon 21000, France.
authors.forename: Aziz
authors.initials: A
authors.lastname: Hichami
authors.affiliation: Physiologie de la Nutrition et Toxicologie, UMR U866 INSERM/Université de Bourgogne/Agro-Sup, 6, Boulevard Gabriel, Dijon 21000, France.
authors.forename: Selvakumar
authors.initials: S
authors.lastname: Subramaniam
authors.affiliation: Physiologie de la Nutrition et Toxicologie, UMR U866 INSERM/Université de Bourgogne/Agro-Sup, 6, Boulevard Gabriel, Dijon 21000, France.
authors.forename: Naim Akhtar
authors.initials: NA
authors.lastname: Khan
authors.affiliation: Laboratoire des Substances Bioactives (LSBA), Centre de Biotechnologie de Borj Cedria, BP-901, 2050 Hammam-Lif, Tunisia.
authors.forename: Ferid
authors.initials: F
authors.lastname: Limam
authors.affiliation: Université de Carthage, Faculté des Sciences de Bizerte, 7021 Jarzouna, Tunisia; Laboratoire des Substances Bioactives (LSBA), Centre de Biotechnologie de Borj Cedria, BP-901, 2050 Hammam-Lif, Tunisia.
authors.forename: Ezzedine
authors.initials: E
authors.lastname: Aouani
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use; D059305:Diet, High-Fat / Q000009:adverse effects*; D056604:Grape Seed Extract / Q000302:isolation & purification / Q000494:pharmacology* / Q000627:therapeutic use; D007249:Inflammation / Q000188:drug therapy / Q000378:metabolism; D008297:Male; D009765:Obesity / Q000188:drug therapy / Q000378:metabolism*; D018384:Oxidative Stress / Q000187:drug effects / Q000502:physiology*; D010179:Pancreas / Q000187:drug effects / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D012639:Seeds; D027843:Vitis
chemical_list: D000975:Antioxidants; D056604:Grape Seed Extract
keywords: Grape seed and skin extract; Inflammation; Obesity; Oxidative stress; Pancreas