title: Biopharmaceuticals from microorganisms: from production to purification.
abstract: The use of biopharmaceuticals dates from the 19th century and within 5-10 years, up to 50% of all drugs in development will be biopharmaceuticals. In the 1980s, the biopharmaceutical industry experienced a significant growth in the production and approval of recombinant proteins such as interferons (IFN α, β, and γ) and growth hormones. The production of biopharmaceuticals, known as bioprocess, involves a wide range of techniques. In this review, we discuss the technology involved in the bioprocess and describe the available strategies and main advances in microbial fermentation and purification process to obtain biopharmaceuticals.
authors.affiliation: Universidade de Sorocaba (UNISO), Departamento de Tecnologia e Processo Ambiental, Sorocaba, SP, Brazil.
authors.forename: Angela Faustino
authors.initials: AF
authors.lastname: Jozala
authors.affiliation: Universidade de Campinas (UNICAMP), Instituto de Biologia, Programa de Pós-Graduação em Biociências e Tecnologia de produtos bioativos, Campinas, SP, Brazil.
authors.forename: Danilo Costa
authors.initials: DC
authors.lastname: Geraldes
authors.affiliation: Universidade de Campinas (UNICAMP), Instituto de Biologia, Programa de Pós-Graduação em Biociências e Tecnologia de produtos bioativos, Campinas, SP, Brazil.
authors.forename: Louise Lacalendola
authors.initials: LL
authors.lastname: Tundisi
authors.affiliation: Universidade de São Paulo, Departamento de Bioquímica e Tecnologia Farmacêutica, São Paulo, SP, Brazil.
authors.forename: Valker de Araújo
authors.initials: VA
authors.lastname: Feitosa
authors.affiliation: Universidade Estadual de São Paulo (UNESP), Instituto de Biociências, Campus do Litoral Paulista, SP, Brazil.
authors.forename: Carlos Alexandre
authors.initials: CA
authors.lastname: Breyer
authors.affiliation: Universidade de Brasília, Faculdade de Ciências da Saúde, Programa de Pós-Graduação em Ciências Farmacêuticas, Brasília, DF, Brazil.
authors.forename: Samuel Leite
authors.initials: SL
authors.lastname: Cardoso
authors.affiliation: Universidade Estadual de Campinas, Faculdade de Ciências Farmacêuticas, Campinas, SP, Brazil.
authors.forename: Priscila Gava
authors.initials: PG
authors.lastname: Mazzola
authors.affiliation: Universidade Estadual de Campinas, Faculdade de Ciências Farmacêuticas, Campinas, SP, Brazil; Universidade Estadual de Campinas, Instituto de Biologia, Departamento de Bioquímica e Biologia Tecidual, Campinas, SP, Brazil.
authors.forename: Laura de
authors.initials: L
authors.lastname: Oliveira-Nascimento
authors.affiliation: Universidade de São Paulo, Departamento de Bioquímica e Tecnologia Farmacêutica, São Paulo, SP, Brazil.
authors.forename: Carlota de Oliveira
authors.initials: CO
authors.lastname: Rangel-Yagui
authors.affiliation: Universidade de Brasília, Faculdade de Ciências da Saúde, Departamento de Farmácia, Brasília, DF, Brazil.
authors.forename: Pérola de Oliveira
authors.initials: PO
authors.lastname: Magalhães
authors.affiliation: Universidade Estadual de São Paulo (UNESP), Instituto de Biociências, Campus do Litoral Paulista, SP, Brazil.
authors.forename: Marcos Antonio de
authors.initials: MA
authors.lastname: Oliveira
authors.affiliation: Universidade de São Paulo, Departamento de Bioquímica e Tecnologia Farmacêutica, São Paulo, SP, Brazil. Electronic address: pessoajr@usp.br.
authors.forename: Adalberto
authors.initials: A
authors.lastname: Pessoa
mesh_terms: D001688:Biological Products; D059451:Biosimilar Pharmaceuticals; D001709:Biotechnology; D004345:Drug Industry; D005285:Fermentation; D006801:Humans; D008828:Microbiological Techniques; D004364:Pharmaceutical Preparations; D011994:Recombinant Proteins
chemical_list: D001688:Biological Products; D059451:Biosimilar Pharmaceuticals; D004364:Pharmaceutical Preparations; D011994:Recombinant Proteins
keywords: Biopharmaceuticals; Biotechnology; Downstream process; Fermentation process; Upstream process