title: Microarray-based analysis and clinical validation identify ubiquitin-conjugating enzyme E2E1 (UBE2E1) as a prognostic factor in acute myeloid leukemia.
abstract: Previous research suggested that single gene expression might be correlated with acute myeloid leukemia (AML) survival. Therefore, we conducted a systematical analysis for AML prognostic gene expressions.
  
  
  
  We performed a microarray-based analysis for correlations between gene expression and adult AML overall survival (OS) using datasets GSE12417 and GSE8970. Positive findings were validated in an independent cohort of 50 newly diagnosed, non-acute promyelocytic leukemia (APL) AML patients by quantitative RT-PCR and survival analysis.
  
  
  
  Microarray-based analysis suggested that expression of eight genes was each associated with 1-year and 3-year AML OS in both GSE12417 and GSE8970 datasets (p < 0.05). Next, we validated our findings in an independent cohort of AML samples collected in our hospital. We found that ubiquitin-conjugating enzyme E2E1 (UBE2E1) expression was adversely correlated with AML survival (p = 0.04). Multivariable analysis showed that UBE2E1highpatients had a significant shorter OS and shorter progression-free survival after adjusting other known prognostic factors (p = 0.03). At last, we found that UBE2E1 expression was negatively correlated with patients' response to induction chemotherapy (p < 0.05).
  
  
  
  In summary, we demonstrated that UBE2E1 expression was a novel prognostic factor in adult, non-APL AML patients.
authors.affiliation: Department of Hematology, Hematology Research Laboratory, West China Hospital, Sichuan University, #37 GuoXue Xiang Street, Chengdu, 610041, China.
authors.forename: Hongmei
authors.initials: H
authors.lastname: Luo
authors.affiliation: Department of Internal Medicine, Weiss Memorial Hospital, University of Illinois, Chicago, IL, USA.
authors.forename: Yu
authors.initials: Y
authors.lastname: Qin
authors.affiliation: Department of Haemotologic Oncology, Taussig Cancer Center, Cleveland Clinic, Cleveland, OH, USA.
authors.forename: Frederic
authors.initials: F
authors.lastname: Reu
authors.affiliation: Sinopec Southwest Company, Chengdu, China.
authors.forename: Sujuan
authors.initials: S
authors.lastname: Ye
authors.affiliation: Department of Hematology, Hematology Research Laboratory, West China Hospital, Sichuan University, #37 GuoXue Xiang Street, Chengdu, 610041, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Dai
authors.affiliation: Department of Hematology, Hematology Research Laboratory, West China Hospital, Sichuan University, #37 GuoXue Xiang Street, Chengdu, 610041, China.
authors.forename: Jingcao
authors.initials: J
authors.lastname: Huang
authors.affiliation: Department of Hematology, Hematology Research Laboratory, West China Hospital, Sichuan University, #37 GuoXue Xiang Street, Chengdu, 610041, China.
authors.forename: Fangfang
authors.initials: F
authors.lastname: Wang
authors.affiliation: Department of Hematology, Hematology Research Laboratory, West China Hospital, Sichuan University, #37 GuoXue Xiang Street, Chengdu, 610041, China.|State Key Laboratory of Biotherapy and Cancer Center, Sichuan University, Chengdu, China.
authors.forename: Dan
authors.initials: D
authors.lastname: Zhang
authors.affiliation: Department of Hematology, Hematology Research Laboratory, West China Hospital, Sichuan University, #37 GuoXue Xiang Street, Chengdu, 610041, China.
authors.forename: Ling
authors.initials: L
authors.lastname: Pan
authors.affiliation: Department of Hematology, Hematology Research Laboratory, West China Hospital, Sichuan University, #37 GuoXue Xiang Street, Chengdu, 610041, China.
authors.forename: Huanling
authors.initials: H
authors.lastname: Zhu
authors.affiliation: Department of Hematology, Hematology Research Laboratory, West China Hospital, Sichuan University, #37 GuoXue Xiang Street, Chengdu, 610041, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Wu
authors.affiliation: Department of Hematology, Hematology Research Laboratory, West China Hospital, Sichuan University, #37 GuoXue Xiang Street, Chengdu, 610041, China.
authors.forename: Ting
authors.initials: T
authors.lastname: Niu
authors.affiliation: Chinese Academy of Medical Sciences, Institute of Hematology and Blood Diseases Hospital, Tianjin, China.
authors.forename: Zhijian
authors.initials: Z
authors.lastname: Xiao
authors.affiliation: Department of Hematology, Hematology Research Laboratory, West China Hospital, Sichuan University, #37 GuoXue Xiang Street, Chengdu, 610041, China. yuhuan_zheng@163.com.|State Key Laboratory of Biotherapy and Cancer Center, Sichuan University, Chengdu, China. yuhuan_zheng@163.com.
authors.forename: Yuhuan
authors.initials: Y
authors.lastname: Zheng
authors.affiliation: Department of Hematology, Hematology Research Laboratory, West China Hospital, Sichuan University, #37 GuoXue Xiang Street, Chengdu, 610041, China. liuting@scu.edu.cn.
authors.forename: Ting
authors.initials: T
authors.lastname: Liu
mesh_terms: D000328:Adult; D018572:Disease-Free Survival; D005260:Female; D020869:Gene Expression Profiling; D006801:Humans; D060828:Induction Chemotherapy; D015470:Leukemia, Myeloid, Acute / Q000175:diagnosis* / Q000188:drug therapy / Q000235:genetics / Q000401:mortality; D015473:Leukemia, Promyelocytic, Acute / Q000175:diagnosis / Q000188:drug therapy / Q000235:genetics / Q000401:mortality; D008297:Male; D046228:Microarray Analysis; D011379:Prognosis; D016019:Survival Analysis; D044763:Ubiquitin-Conjugating Enzymes / Q000032:analysis*
chemical_list: C489963:UBE2E1 protein, human; D044763:Ubiquitin-Conjugating Enzymes
keywords: Acute myeloid leukemia; Prognosis; UBE2E1