title: In vitroEffectiveness of Commercial Bacteriophage Cocktails on Diverse Extended-Spectrum Beta-Lactamase ProducingEscherichia coliStrains.
abstract: The objective of this study is to determine thein vitrosusceptibility of Georgian bacteriophage cocktails on multidrug resistant (MDR) extended-spectrum beta-lactamase producingEscherichia coli(ESBL-EC) isolated from patients' blood and urine cultures. A total of 615E. coliisolates were included in this study. Phene Plate (PhP)-typing and phylogenetic grouping were used for the typing. Antimicrobial resistance profiles and ESBL production of all isolates were confirmed according to Clinical and Laboratory Standards Institute (CLSI) criteria. The activities of four bacteriophage cocktails (Enko-phage, SES-bacteriophage, Pyo-bacteriophage, and Intesti-bacteriophage) were determined against 142 ESBL-EC usingin vitrospot tests. According to this, Enko-phage were active against 87.3% of the tested strains while that ratio was 81.7% for Intesti-bacteriophage, 81.7% for Pyo-bacteriophage, and 59.2% for SES-bacteriophage cocktails. Based on the contingency tests, the phage cocktails were observed to be statistically significantly (p< 0.001) more effective on ESBL-EC strains belonging to phylogenetic groups D and B2. The employed phage cocktails were found to be affective against all tested resistant types. These results are promising especially for the infections that are caused by MDR pathogens that are difficult to treat. As this is a preliminary step to the potential clinical trials to be designed for the country,in vitroconfirmation of their success on a MDR ESBL-EC collection should be accepted as an initial action, which is encouraging to consider clinical trials of phage therapy especially in countries which are not introduce phage therapy.
authors.affiliation: Department of Microbiology and Clinical Microbiology, Faculty of Medicine, Erciyes UniversityKayseri, Turkey; Genome and Stem Cell Center (GENKOK), Erciyes UniversityKayseri, Turkey.
authors.forename: Aycan
authors.initials: A
authors.lastname: Gundogdu
authors.affiliation: Laboratory of Molecular Biology, G. Eliava Institute of Bacteriophages, Microbiology and Virology Tbilisi, Georgia.
authors.forename: Darajen
authors.initials: D
authors.lastname: Bolkvadze
authors.affiliation: Department of Microbiology and Clinical Microbiology, Faculty of Medicine, Erciyes University Kayseri, Turkey.
authors.forename: Huseyin
authors.initials: H
authors.lastname: Kilic
keywords: ESBL-E. coli; antibiotic resistance; bacteriophage; phage therapy; phylogenetic grouping