title: Allele-Specific Phenotype Suggests a Possible Stimulatory Activity of RCAN-1 on Calcineurin inCaenorhabditis elegans.
abstract: Regulator of calcineurin 1 (RCAN1) binds to calcineurin through the PxIxIT motif, which is evolutionarily conserved. SP repeat phosphorylation in RCAN1 is required for its complete function. The specific interaction between RCAN1 and calcineurin is critical for calcium/calmodulin-dependent regulation of calcineurin serine/threonine phosphatase activity. In this study, we investigated two available deletionrcan-1mutants inCaenorhabditis elegans, which proceed differently for transcription and translation. We found thatrcan-1may be required for calcineurin activity and possess calcineurin-independent function in body growth and egg-laying behavior. In the genetic background of enhanced calcineurin activity, thercan-1mutant expressing a truncated RCAN-1 which retains the calcineurin-binding PxIxIT motif but misses SP repeats stimulated growth, whilercan-1lack mutant resulted in hyperactive egg-laying suppression. These data suggestrcan-1has unknown functions independent of calcineurin, and may be a stimulatory calcineurin regulator under certain circumstances.
authors.affiliation: Department of Life Science, Hanyang University, Seoul 04763, Korea.|BK21 PLUS Life Science for BDR Team, Hanyang University, Seoul 04763, Korea.|Research Institute for Natural Sciences, Hanyang University, Seoul 04763, Korea.
authors.forename: Weixun
authors.initials: W
authors.lastname: Li
authors.affiliation: Department of Life Science, Hanyang University, Seoul 04763, Korea.|BK21 PLUS Life Science for BDR Team, Hanyang University, Seoul 04763, Korea.|Research Institute for Natural Sciences, Hanyang University, Seoul 04763, Korea.
authors.forename: Tae-Woo
authors.initials: TW
authors.lastname: Choi
authors.affiliation: Department of Life Science, Hanyang University, Seoul 04763, Korea.|BK21 PLUS Life Science for BDR Team, Hanyang University, Seoul 04763, Korea.|Research Institute for Natural Sciences, Hanyang University, Seoul 04763, Korea.
authors.forename: Joohong
authors.initials: J
authors.lastname: Ahnn
authors.affiliation: Department of Life Science, Hanyang University, Seoul 04763, Korea.|BK21 PLUS Life Science for BDR Team, Hanyang University, Seoul 04763, Korea.|Research Institute for Natural Sciences, Hanyang University, Seoul 04763, Korea.
authors.forename: Sun-Kyung
authors.initials: SK
authors.lastname: Lee
mesh_terms: D000483:Alleles; D000818:Animals; D030801:Animals, Genetically Modified; D017173:Caenorhabditis elegans / Q000235:genetics* / Q000378:metabolism; D029742:Caenorhabditis elegans Proteins / Q000235:genetics* / Q000378:metabolism; D019703:Calcineurin / Q000235:genetics* / Q000378:metabolism; D047908:Intracellular Signaling Peptides and Proteins / Q000235:genetics* / Q000378:metabolism; D009124:Muscle Proteins / Q000235:genetics* / Q000378:metabolism; D010641:Phenotype; D010766:Phosphorylation; D015398:Signal Transduction
chemical_list: D029742:Caenorhabditis elegans Proteins; D047908:Intracellular Signaling Peptides and Proteins; D009124:Muscle Proteins; D019703:Calcineurin
keywords: C. elegans; RCAN; calcineurin