title: Molecular Detection of Resistance to Azole Components.
abstract: Fungal infections have increased significantly in the last few years, and their outcomes are in part complicated by the emergence of antifungal drug-resistant pathogens. Together with Candida species, the mould Aspergillus fumigatus is one of the most prevalent organisms to cause invasive fungal disease. The molecular detection of (tri)azole resistance in both Candida and Aspergillus species may represent a useful means of monitoring the incidence of clinical isolates with antifungal resistance-associated gene alterations. Here, we describe molecular methods that have been developed to allow for accurate detection of azole-resistant isolates among C. glabrata and A. fumigatus fungal species.
authors.affiliation: Section of Hygiene, Institute of Public Health, Università Cattolica del Sacro Cuore, Largo Francesco Vito 1, Rome, 00168, Italy. brunella.posteraro@unicatt.it.
authors.forename: Brunella
authors.initials: B
authors.lastname: Posteraro
authors.affiliation: Institute of Microbiology, Università Cattolica del Sacro Cuore, Rome, Italy.
authors.forename: Antonietta
authors.initials: A
authors.lastname: Vella
authors.affiliation: Institute of Microbiology, Università Cattolica del Sacro Cuore, Rome, Italy.
authors.forename: Elena
authors.initials: E
authors.lastname: De Carolis
authors.affiliation: Institute of Microbiology, Università Cattolica del Sacro Cuore, Rome, Italy.
authors.forename: Maurizio
authors.initials: M
authors.lastname: Sanguinetti
mesh_terms: D000935:Antifungal Agents / Q000494:pharmacology*; D001228:Aspergillosis / Q000175:diagnosis / Q000188:drug therapy / Q000382:microbiology*; D001230:Aspergillus / Q000187:drug effects / Q000235:genetics; D001393:Azoles / Q000494:pharmacology*; D002175:Candida / Q000187:drug effects; D002177:Candidiasis / Q000175:diagnosis / Q000188:drug therapy / Q000382:microbiology*; D025141:Drug Resistance, Fungal / Q000235:genetics*; D005800:Genes, Fungal; D006801:Humans; D008826:Microbial Sensitivity Tests; D025202:Molecular Diagnostic Techniques; D060888:Real-Time Polymerase Chain Reaction; D017422:Sequence Analysis, DNA
chemical_list: D000935:Antifungal Agents; D001393:Azoles
keywords: Antifungal drug resistance; Fungal pathogens; Gene sequencing; Molecular analysis; Quantitative real-time RT-PCR