title: Liquid lipases for enzymatic concentration of n-3 polyunsaturated fatty acids in monoacylglycerols via ethanolysis: Catalytic specificity and parameterization.
abstract: This work examined catalytic specificity and fatty acid selectivity of five liquid lipases C. antarctica lipase A and B (CAL-A/B), and lipase TL (T. lanuginosus), Eversa Transfrom and NS in ethanolysis of fish oil with the aim to concentrate n-3 PUFAs into monoacylglycerols (MAGs) products. Lipase TL, Eversa Transform & NS entail a much faster reaction and produce higher MAGs yield (>30%); whereas CAL-A obtains the highest concentration of n-3 PUFAs/DHA/EPA into MAGs products (88.30%); followed by lipase NS (81.02%).13C NMR analysis indicates that CAL-B and lipase TL are sn-1,3 specific; but CAL-A and lipase Eversa Transform are non-regiospecific or weak sn-2 specific; which plausibly explains high enrichment effect of the latter two lipases. All liquid lipases are observed reusable for a certain times (lipase Eversa Transform up to 12 times), demonstrating their competitive advantage over immobilized form for industrial application because of their higher activity and cheaper operation cost.
authors.affiliation: Department of Engineering, Aarhus University, Gustav Wieds Vej 10, 8000 Aarhus C, Denmark; College of Life Science, Fujian Normal University, Fuzhou 350117, China.
authors.forename: Yongjin
authors.initials: Y
authors.lastname: He
authors.affiliation: Department of Engineering, Aarhus University, Gustav Wieds Vej 10, 8000 Aarhus C, Denmark.
authors.forename: Jingbo
authors.initials: J
authors.lastname: Li
authors.affiliation: Department of Engineering, Aarhus University, Gustav Wieds Vej 10, 8000 Aarhus C, Denmark.
authors.forename: Sitharam
authors.initials: S
authors.lastname: Kodali
authors.affiliation: Novozymes A/S DK, Krogshojvej 36, 2880 Bagsvaerd, Denmark.
authors.forename: Thomas
authors.initials: T
authors.lastname: Balle
authors.affiliation: College of Life Science, Fujian Normal University, Fuzhou 350117, China; Engineering Research Center of Industrial Microbiology of Ministry of Education, Fujian Normal University, Fuzhou 350117, China.
authors.forename: Bilian
authors.initials: B
authors.lastname: Chen
authors.affiliation: Department of Engineering, Aarhus University, Gustav Wieds Vej 10, 8000 Aarhus C, Denmark. Electronic address: guo@eng.au.dk.
authors.forename: Zheng
authors.initials: Z
authors.lastname: Guo
mesh_terms: D000818:Animals; D001203:Ascomycota / Q000201:enzymology; D002175:Candida / Q000201:enzymology; D002384:Catalysis; D000431:Ethanol / Q000737:chemistry; D015525:Fatty Acids, Omega-3 / Q000737:chemistry*; D005395:Fish Oils / Q000737:chemistry*; D005656:Fungal Proteins / Q000737:chemistry*; D008049:Lipase / Q000737:chemistry*; D050178:Monoglycerides / Q000737:chemistry
chemical_list: D015525:Fatty Acids, Omega-3; D005395:Fish Oils; D005656:Fungal Proteins; D050178:Monoglycerides; D000431:Ethanol; D008049:Lipase
keywords: Candida antarctica lipase A (CAL-A); Catalytic specificity; Concentration effect; Liquid lipase; Monoacylglycerols (MAGs); n-3 polyunsaturated fatty acids (n-3 PUFAs)