title: Assessing the effects of threonyl-tRNA synthetase on angiogenesis-related responses.
abstract: Several recent reports have found a connection between specific aminoacyl-tRNA synthetases and the regulation of angiogenesis. As this new area of research is explored, it is important to have reliable assays to assess the specific angiogenesis functions of these enzymes. This review provides information about specific in vitro and in vivo methods that were used to assess the angiogenic functions of threonyl-tRNA synthetase including endothelial cell migration and tube assays as well as chorioallantoic membrane and tumor vascularization assays. The theory and discussion include best methods of analysis and quantification along with the advantages and limitations of each type of assay.
authors.affiliation: Department of Pharmacology, College of Medicine, University of Vermont, Burlington, VT 05405, United States.
authors.forename: Adam C
authors.initials: AC
authors.lastname: Mirando
authors.affiliation: Department of Pharmacology, College of Medicine, University of Vermont, Burlington, VT 05405, United States.
authors.forename: Khadar
authors.initials: K
authors.lastname: Abdi
authors.affiliation: Department of Pharmacology, College of Medicine, University of Vermont, Burlington, VT 05405, United States.
authors.forename: Peibin
authors.initials: P
authors.lastname: Wo
authors.affiliation: Department of Pharmacology, College of Medicine, University of Vermont, Burlington, VT 05405, United States. Electronic address: Karen.lounsbury@uvm.edu.
authors.forename: Karen M
authors.initials: KM
authors.lastname: Lounsbury
mesh_terms: D000818:Animals; D001681:Biological Assay; D014408:Biomarkers, Tumor / Q000235:genetics / Q000378:metabolism; D002465:Cell Movement / Q000187:drug effects; D002642:Chick Embryo; D049033:Chorioallantoic Membrane / Q000098:blood supply / Q000187:drug effects* / Q000201:enzymology; D003094:Collagen / Q000737:chemistry; D004338:Drug Combinations; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D005260:Female; D005786:Gene Expression Regulation; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D007797:Laminin / Q000737:chemistry; D051379:Mice; D009389:Neovascularization, Pathologic / Q000188:drug therapy* / Q000201:enzymology / Q000235:genetics / Q000473:pathology; D018919:Neovascularization, Physiologic / Q000187:drug effects / Q000235:genetics; D010051:Ovarian Neoplasms / Q000098:blood supply / Q000188:drug therapy* / Q000235:genetics / Q000473:pathology; D019408:Platelet Endothelial Cell Adhesion Molecule-1 / Q000235:genetics / Q000378:metabolism; D011509:Proteoglycans / Q000737:chemistry; D034741:RNA, Small Interfering / Q000235:genetics / Q000378:metabolism; D012363:RNA, Transfer, Thr / Q000235:genetics / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D013914:Threonine-tRNA Ligase / Q000037:antagonists & inhibitors* / Q000235:genetics / Q000378:metabolism; D046249:Transfer RNA Aminoacylation; D023041:Xenograft Model Antitumor Assays
chemical_list: D014408:Biomarkers, Tumor; D004338:Drug Combinations; D004791:Enzyme Inhibitors; D007797:Laminin; D019408:Platelet Endothelial Cell Adhesion Molecule-1; D011509:Proteoglycans; D034741:RNA, Small Interfering; D012363:RNA, Transfer, Thr; D011993:Recombinant Fusion Proteins; C059485:matrigel; D003094:Collagen; D013914:Threonine-tRNA Ligase
keywords: Aminoacyl-tRNA synthetase; Angiogenesis methods; CAM assay; Endothelial cells; ImageJ; MetaMorph