title: Isolation and characterization of a high-efficiency erythromycin A-degrading Ochrobactrum sp. strain.
abstract: In this work, Erythromycin A(EA)- degrading bacteria was isolated from the contaminated soil obtained from a pharmaceutical factory in China. The isolate designated as strain WX-J1 was identified as Ochrobactrum sp. by sequence analysis of its 16S rDNA gene. It can grow in a medium containing EA as the sole source of carbon and its optimal growth pH and temperature were 6.5 and 32°C, respectively. Under these conditions, when the initial Erythromycin A concentration was 100mg·L-1, 97% of Erythromycin A has been degraded. HPLC-MS analyses indicated that Erythromycin A degradation produced intermediates contained the following three substances: 3-depyranosyloxy erythromycin A, 7,12-dyhydroxy-6-deoxyerythronolide B, 6-deoxyerythronolide B and propionaldehyde. Since Erythromycin A-degrading Ochrobactrum sp. strain rapidly degraded Erythromycin A, this strain might be useful for bioremediation purposes.
authors.affiliation: School of Chemistry and Biology Engineering, University of Science and Technology, Beijing 100083, China.
authors.forename: Weiwei
authors.initials: W
authors.lastname: Zhang
authors.affiliation: School of Chemistry and Biology Engineering, University of Science and Technology, Beijing 100083, China.
authors.forename: Lina
authors.initials: L
authors.lastname: Qiu
authors.affiliation: School of Chemistry and Biology Engineering, University of Science and Technology, Beijing 100083, China. Electronic address: gongaijun5661@ustb.edu.cn.
authors.forename: Aijun
authors.initials: A
authors.lastname: Gong
authors.affiliation: School of Chemistry and Biology Engineering, University of Science and Technology, Beijing 100083, China.
authors.forename: Xiaotao
authors.initials: X
authors.lastname: Yuan
mesh_terms: D001673:Biodegradation, Environmental; D002681:China; D004275:DNA, Ribosomal / Q000235:genetics; D004785:Environmental Pollutants / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D004917:Erythromycin / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D015394:Molecular Structure; D042041:Ochrobactrum / Q000302:isolation & purification* / Q000378:metabolism; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012988:Soil Microbiology; D013696:Temperature
chemical_list: D004275:DNA, Ribosomal; D004785:Environmental Pollutants; D012336:RNA, Ribosomal, 16S; D004917:Erythromycin
keywords: Biodegradation; Bioremediation; Erythromycin a; Ochrobactrum sp.