title: Disbalance of calcium regulation-related genes in broiler hearts induced by selenium deficiency.
abstract: Dietary selenium (Se) deficiency may influence the calcium (Ca) homeostasis in broilers. Our objective was to investigate the effects of Se deficiency on Ca regulation-related genes in broiler hearts. In the present study, 1-day-old broilers were fed either a commercial diet (as control group) with 0.15 mg/kg Se or a Se-deficient diet (as L group) with 0.033 mg/kg Se for 35 days. We examined the mRNA expression levels of 15 Ca regulation-related genes (ITPR 1, ITPR 2, ITPR3, RyR2, RyR3, SERCA1s, SLC8A1, PMCA1, CACNA1S, TRPC1, TRPC3, stromal interacting molecule 1, ORAI1, calmodulin (CaLM) and calreticulin (CRT) in broiler hearts. Then, Kyoto Encyclopedia of Genes and Genomes analysis, protein-protein interactions (PPI) analysis and correlation analysis were performed to analyse the relationships between these genes. The results showed that the mRNA expression levels of ITPR 1, ITPR 2, RyR2, RyR3, SERCA1s, SLC8A1, PMCA1, CACNA1S, CaLM and CRT were generally decreased by Se deficiency, while mRNA expression levels of TRPC1, TRPC3, stromal interacting molecule 1, ORAI1 and ITPR3 were increased by Se deficiency. Kyoto Encyclopedia of Genes and Genomes and PPI analysis showed that these Ca regulation-related genes are involved in the Ca signalling pathway and a total of 15 PPIs with a combined score of >0.4 were obtained. In conclusion, the results demonstrated that Se deficiency might cause heart injury via modulating the Ca-related pathway genes, and then induce Ca2+overload in the heart of broilers.
authors.affiliation: a College of Veterinary Medicine , Northeast Agricultural University , Harbin , People's Republic of China.
authors.forename: Ziwei
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: a College of Veterinary Medicine , Northeast Agricultural University , Harbin , People's Republic of China.
authors.forename: Man
authors.initials: M
authors.lastname: Liu
authors.affiliation: a College of Veterinary Medicine , Northeast Agricultural University , Harbin , People's Republic of China.
authors.forename: Zhenqiong
authors.initials: Z
authors.lastname: Guan
authors.affiliation: a College of Veterinary Medicine , Northeast Agricultural University , Harbin , People's Republic of China.
authors.forename: Jie
authors.initials: J
authors.lastname: Yang
authors.affiliation: b College of Life Science , Northeast Agricultural University , Harbin , People's Republic of China.
authors.forename: Zhonghua
authors.initials: Z
authors.lastname: Liu
authors.affiliation: a College of Veterinary Medicine , Northeast Agricultural University , Harbin , People's Republic of China.
authors.forename: Shiwen
authors.initials: S
authors.lastname: Xu
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002645:Chickens / Q000235:genetics / Q000378:metabolism*; D004032:Diet / Q000662:veterinary; D019587:Dietary Supplements; D005786:Gene Expression Regulation; D006706:Homeostasis; D009206:Myocardium / Q000378:metabolism; D018384:Oxidative Stress; D012643:Selenium / Q000172:deficiency* / Q000378:metabolism
chemical_list: D012643:Selenium; D002118:Calcium
keywords: Selenium deficiency; broiler; calcium; calcium regulation-related genes; heart