title: Oxidative DNA Damage to Sperm Cells and Peripheral Blood Leukocytes in Infertile Men.
abstract: BACKGROUND Oxidative DNA damage is associated with male infertility. The aim of this study was to evaluate the oxidative DNA damage of sperm cells and blood leukocytes and to determine the levels of MDA and NO levels in seminal and blood plasma of idiopathic infertile men. MATERIAL AND METHODS The study enrolled 52 patients, including 30 infertile and 22 fertile men. MDA, NO, and 8-OHdG/106dG were estimated using spectrophotometry and high-pressure liquid chromatography (HPLC)-based methods in seminal and blood plasma. The association with the sperm parameters was assessed, particularly sperm counts and motility. RESULTS The mean sperm concentration and sperm motility of the fertile men were significantly higher than that of the infertile men. The mean MDA and NO concentration in the seminal and blood samples of the infertile men were higher than that of fertile men. Also, the mean numbers of sperm cells and leukocytes 8-OHdG/106dG of the infertile men were significantly higher than that of fertile men (p=0.04 and p<0.001, respectively). Sperm motility and sperm count were negatively correlated with leukocyte and sperm cell 8-OHdG/106dG ratio. However, progressive motility was significantly negatively correlated with sperm cell and leukocyte 8-OHdG/106dG ratio (R=-0.357, p=0.026; R=-0.388, p=0.024, respectively). CONCLUSIONS Oxidative stress is an important factor in male infertility. Therefore, biochemical detection of 8-OHdG/106dG in sperm cells and blood leukocytes may be an additional tool in the diagnosis of male infertility.
authors.affiliation: Department of Urology, Yüzüncü Yıl University, Van, Turkey.
authors.forename: Kerem
authors.initials: K
authors.lastname: Taken
authors.affiliation: Department of Clinical Biochemistry, Yüzüncü Yıl University, Van, Turkey.
authors.forename: Hamit Hakan
authors.initials: HH
authors.lastname: Alp
authors.affiliation: Department of Urology, State Hospital, Bitlis, Turkey.
authors.forename: Recep
authors.initials: R
authors.lastname: Eryilmaz
authors.affiliation: Department of Urology, Istanbul University, Istanbul, Turkey.
authors.forename: Muhammet Irfan
authors.initials: MI
authors.lastname: Donmez
authors.affiliation: Department of Urology, Yüzüncü Yıl University, Van, Turkey.
authors.forename: Murat
authors.initials: M
authors.lastname: Demir
authors.affiliation: Department of Urology, Yüzüncü Yıl University, Van, Turkey.
authors.forename: Mustafa
authors.initials: M
authors.lastname: Gunes
authors.affiliation: Department of Urology, Research Hospital, Batman, Turkey.
authors.forename: Rahmi
authors.initials: R
authors.lastname: Aslan
authors.affiliation: Department of Clinical Biochemistry, Yüzüncü Yıl University, Van, Turkey.
authors.forename: Mehmet Ramazan
authors.initials: MR
authors.lastname: Sekeroglu
mesh_terms: D000328:Adult; D000975:Antioxidants / Q000378:metabolism; D016022:Case-Control Studies; D004249:DNA Damage; D006801:Humans; D007248:Infertility, Male / Q000097:blood / Q000235:genetics* / Q000378:metabolism* / Q000473:pathology; D007962:Leukocytes / Q000378:metabolism* / Q000473:pathology; D008297:Male; D018384:Oxidative Stress / Q000235:genetics / Q000502:physiology*; D013076:Sperm Count; D013081:Sperm Motility / Q000502:physiology; D013094:Spermatozoa / Q000378:metabolism* / Q000473:pathology*; D014421:Turkey
chemical_list: D000975:Antioxidants
keywords: DNA Damage; Infertility, Male; Oxidative Stress; Sperm Motility