title: Tissue distribution of capilliposide B, capilliposide C and their bioactive metabolite in mice using liquid -tandem mass spectrometry.
abstract: Lysimachia capillipes Hemsl (Primulaceae), a folk medicinal plant in China, showed significant anti-tumor activities in vivo and in vitro. Capilliposide B (LC-B) and capilliposide C (LC-C) are the main bioactive components in this plant. To explore their tissue distribution, a reliable bioanalytical method for the quantification of LC-B, LC-C and their bioactive metabolite, capilliposide A (LC-A), in mouse tissues was developed and validated. In this study, the tissue distribution profiles of the three compounds were examined after intravenous administration of pure LC-B and oral administration of total saponins of L. capillipes Hemsl extract (LCE) for 10 days. Method validation was conducted over the curve range 10.0-5000 ng/mL for all three analytes in various tissue homogenates. The relative standard deviation of intra-day and inter-day precision of the QC samples was <14.7%, and the accuracy ranged from 85.9 to 114.0%. The results indicated that LC-B was rapidly and widely distributed throughout the whole body except for muscle following intravenous administration of LC-B. In addition, LC-A was only in liver, intestine, lung and stomach. After oral administration of LCE, LC-B and LC-C were distributed into various tissues. The highest levels were observed in stomach and intestine.
authors.affiliation: School of Pharmacy, Weifang Medical University, Weifang, Shandong, China.
authors.forename: Zhongzhe
authors.initials: Z
authors.lastname: Cheng
authors.affiliation: Tongji School of Pharmacy, Huazhong University of Science and Technology, Wuhan, Hubei, China.
authors.forename: Xing
authors.initials: X
authors.lastname: Zhou
authors.affiliation: Tongji School of Pharmacy, Huazhong University of Science and Technology, Wuhan, Hubei, China.
authors.forename: Bingying
authors.initials: B
authors.lastname: Hu
authors.affiliation: Tongji School of Pharmacy, Huazhong University of Science and Technology, Wuhan, Hubei, China.
authors.forename: Wenyi
authors.initials: W
authors.lastname: Li
authors.affiliation: Tongji School of Pharmacy, Huazhong University of Science and Technology, Wuhan, Hubei, China.
authors.forename: Guiying
authors.initials: G
authors.lastname: Chen
authors.affiliation: Department of Pharmacy, Tongji Hospital of Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Biomedical Engineering, Zhejiang University, Hangzhou, Zhejiang, China.
authors.forename: Jingkui
authors.initials: J
authors.lastname: Tian
authors.affiliation: Department of Biomedical Engineering, Zhejiang University, Hangzhou, Zhejiang, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, China.
authors.forename: Ming
authors.initials: M
authors.lastname: Li
authors.affiliation: Tongji School of Pharmacy, Huazhong University of Science and Technology, Wuhan, Hubei, China.
authors.forename: Hongliang
authors.initials: H
authors.lastname: Jiang
mesh_terms: D000818:Animals; D002853:Chromatography, Liquid / Q000379:methods*; D051379:Mice; D012503:Saponins / Q000493:pharmacokinetics*; D053719:Tandem Mass Spectrometry / Q000379:methods*; D014018:Tissue Distribution; D014315:Triterpenes / Q000493:pharmacokinetics*
chemical_list: D012503:Saponins; D014315:Triterpenes; C511099:capilliposide B; C511098:capilliposide C
keywords: LC-MS/MS; Lysimachia capillipes Hemsl; capilliposide B; capilliposide C; tissue distribution