title: Mitochondrial genotyping of an endangered bitterlingAcheilognathus typus(Cyprinidae).
abstract: Genotyping of endangered species is helpful for establishing and evaluating conservation strategies. Mitochondrial sequence data was analyzed from 541 individuals of a critically endangered fish,Acheilognathus typusfrom present-day range-wide localities to re-evaluate an in-progress restoration program around Lake Izunuma-Uchinuma, Miyagi, Japan.Acheilognathus typusshowed low sequence diversity with only eight haplotypes and π and ĥ values of 0.59129 and 0.00118 respectively. Genetic data suggestsAcheilognathus typusis adapted to pulsed environments and prone to population flush and crash. Genotyping of populations in introduced localities revealed that their source is not from nearby localities.
authors.affiliation: National Research Institute of Fisheries Science, Japan Fisheries Research and Education Agency, Fukuura 2-12-4, Kanazawa, Yokohama 236-8648, Japan.
authors.forename: Kenji
authors.initials: K
authors.lastname: Saitoh
authors.affiliation: The Miyagi Prefectural Izunuma-Uchinuma Environmental Foundation, Shikimi 17-2, Kamihataoka, Wakayanagi, Kurihara, Miyagi 989-5504, Japan; Present address: Central Japan Bitterling Research Association, Hayashi 7-178-3-A503, Ogaki, Gifu 503-0015, Japan.
authors.forename: Kentaro
authors.initials: K
authors.lastname: Shindo
authors.affiliation: The Miyagi Prefectural Izunuma-Uchinuma Environmental Foundation, Shikimi 17-2, Kamihataoka, Wakayanagi, Kurihara, Miyagi 989-5504, Japan.
authors.forename: Yasufumi
authors.initials: Y
authors.lastname: Fujimoto
authors.affiliation: Society for Shinaimotsugo Conservation, Koyachi 504-1, Kashimadai, Osaki, Miyagi 989-4102, Japan.
authors.forename: Kiyotaka
authors.initials: K
authors.lastname: Takahashi
authors.affiliation: The Miyagi Prefectural Izunuma-Uchinuma Environmental Foundation, Shikimi 17-2, Kamihataoka, Wakayanagi, Kurihara, Miyagi 989-5504, Japan.
authors.forename: Tetsuo
authors.initials: T
authors.lastname: Shimada
keywords: Bottleneck; Cyprinidae; conservation genetics; fish; heteroplasmy; invasive alien species; restoration