title: The Chromatin Remodeler ISW1 Is a Quality Control Factor that Surveys Nuclear mRNP Biogenesis.
abstract: Chromatin dynamics play an essential role in regulating DNA transaction processes, but it is unclear whether transcription-associated chromatin modifications control the mRNA ribonucleoparticles (mRNPs) pipeline from synthesis to nuclear exit. Here, we identify the yeast ISW1 chromatin remodeling complex as an unanticipated mRNP nuclear export surveillance factor that retains export-incompetent transcripts near their transcription site. This tethering activity of ISW1 requires chromatin binding and is independent of nucleosome sliding activity or changes in RNA polymerase II processivity. Combination of in vivo UV-crosslinking and genome-wide RNA immunoprecipitation assays show that Isw1 and its cofactors interact directly with premature mRNPs. Our results highlight that the concerted action of Isw1 and the nuclear exosome ensures accurate surveillance mechanism that proofreads the efficiency of mRNA biogenesis.
authors.affiliation: Université Paris Diderot, Sorbonne Paris Cité, INSERM UMR944, CNRS UMR7212, Hôpital St. Louis 1, Avenue Claude Vellefaux, 75475 Paris Cedex, France. Electronic address: anna.babour@inserm.fr.
authors.forename: Anna
authors.initials: A
authors.lastname: Babour
authors.affiliation: Université Paris Diderot, Sorbonne Paris Cité, INSERM UMR944, CNRS UMR7212, Hôpital St. Louis 1, Avenue Claude Vellefaux, 75475 Paris Cedex, France.
authors.forename: Qingtang
authors.initials: Q
authors.lastname: Shen
authors.affiliation: Université Paris Diderot, Sorbonne Paris Cité, INSERM UMR944, CNRS UMR7212, Hôpital St. Louis 1, Avenue Claude Vellefaux, 75475 Paris Cedex, France.
authors.forename: Julien
authors.initials: J
authors.lastname: Dos-Santos
authors.affiliation: Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, UK.
authors.forename: Struan
authors.initials: S
authors.lastname: Murray
authors.affiliation: Université Paris Diderot, Sorbonne Paris Cité, INSERM UMR944, CNRS UMR7212, Hôpital St. Louis 1, Avenue Claude Vellefaux, 75475 Paris Cedex, France.
authors.forename: Alexandre
authors.initials: A
authors.lastname: Gay
authors.affiliation: Institut Jacques Monod, Université Paris Diderot, Sorbonne Paris Cité, CNRS, Bâtiment Buffon, 15 rue Hélène Brion, 75205 Paris Cedex, France.
authors.forename: Drice
authors.initials: D
authors.lastname: Challal
authors.affiliation: Department of Biochemistry, Emory University School of Medicine, Atlanta, GA 30322, USA.
authors.forename: Milo
authors.initials: M
authors.lastname: Fasken
authors.affiliation: Institut Jacques Monod, Université Paris Diderot, Sorbonne Paris Cité, CNRS, Bâtiment Buffon, 15 rue Hélène Brion, 75205 Paris Cedex, France.
authors.forename: Benoît
authors.initials: B
authors.lastname: Palancade
authors.affiliation: Department of Biochemistry, Emory University School of Medicine, Atlanta, GA 30322, USA.
authors.forename: Anita
authors.initials: A
authors.lastname: Corbett
authors.affiliation: Institut Jacques Monod, Université Paris Diderot, Sorbonne Paris Cité, CNRS, Bâtiment Buffon, 15 rue Hélène Brion, 75205 Paris Cedex, France.
authors.forename: Domenico
authors.initials: D
authors.lastname: Libri
authors.affiliation: Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, UK.
authors.forename: Jane
authors.initials: J
authors.lastname: Mellor
authors.affiliation: Université Paris Diderot, Sorbonne Paris Cité, INSERM UMR944, CNRS UMR7212, Hôpital St. Louis 1, Avenue Claude Vellefaux, 75475 Paris Cedex, France.
authors.forename: Catherine
authors.initials: C
authors.lastname: Dargemont
mesh_terms: D000251:Adenosine Triphosphatases / Q000378:metabolism*; D042002:Chromatin Assembly and Disassembly; D004268:DNA-Binding Proteins / Q000378:metabolism*; D063326:Exosome Multienzyme Ribonuclease Complex / Q000378:metabolism; D055354:Exosomes / Q000378:metabolism; D046912:Multiprotein Complexes / Q000378:metabolism; D012319:RNA Polymerase II / Q000378:metabolism; D012261:Ribonucleoproteins / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism*
chemical_list: D004268:DNA-Binding Proteins; D046912:Multiprotein Complexes; D012261:Ribonucleoproteins; D029701:Saccharomyces cerevisiae Proteins; C026452:messenger ribonucleoprotein; D012319:RNA Polymerase II; D063326:Exosome Multienzyme Ribonuclease Complex; C112610:RRP6 protein, S cerevisiae; D000251:Adenosine Triphosphatases; C470546:ISW1 protein, S cerevisiae
keywords: ISWI; Isw1; Npl3; chromatin remodeler; mRNA export; mRNP biogenesis; mRNP quality control