title: Liposome Biodistribution via Europium Complexes.
abstract: The drug delivery field needs tools to follow vector biodistribution. Radioactive tracers and conventional fluorophores are widely used. We propose here to use europium complexes. Use of pulsed light source time-resolved fluorimetry takes into account the fluorescence decay time of the lanthanide chelates to gain sensitivity in biological media. The method was developed to follow liposome biodistribution. Octadecyl-DTPA.Eu compound has been prepared and incorporated into liposomes without alteration of its fluorescence signal. The method has been validated by comparison with fluorophore-labeled liposomes. The way to proceed to use this method for liposomes or other vectors is detailed.
authors.affiliation: Chimie ParisTech, PSL Research University, Unité de Technologies Chimiques et Biologiques pour la Santé (UTCBS), 4, Avenue de l'Observatoire, F-75005, Paris, France. nathalie.mignet@parisdescartes.fr.|CNRS, UTCBS UMR 8258, Paris, F-75006, France. nathalie.mignet@parisdescartes.fr.|Université Paris Descartes, Sorbonne-Paris-Cité, UTCBS, Paris, F-75006, France. nathalie.mignet@parisdescartes.fr.|INSERM, UTCBS U 1022, Paris, F-75006, France. nathalie.mignet@parisdescartes.fr.
authors.forename: Nathalie
authors.initials: N
authors.lastname: Mignet
authors.affiliation: Chimie ParisTech, PSL Research University, Unité de Technologies Chimiques et Biologiques pour la Santé (UTCBS), 4, Avenue de l'Observatoire, F-75005, Paris, France.|CNRS, UTCBS UMR 8258, Paris, F-75006, France.|Université Paris Descartes, Sorbonne-Paris-Cité, UTCBS, Paris, F-75006, France.|INSERM, UTCBS U 1022, Paris, F-75006, France.
authors.forename: Daniel
authors.initials: D
authors.lastname: Scherman
mesh_terms: D000818:Animals; D002021:Buffers; D005063:Europium / Q000737:chemistry*; D008081:Liposomes / Q000737:chemistry*; D008810:Mice, Inbred C57BL; D008856:Microscopy, Fluorescence; D004369:Pentetic Acid / Q000737:chemistry; D013194:Staining and Labeling; D014018:Tissue Distribution
chemical_list: D002021:Buffers; D008081:Liposomes; D005063:Europium; D004369:Pentetic Acid
keywords: Europium; Fluorescence; Liposomes; Pharmacokinetics; Time-resolved fluorimetry