title: Synchrotron radiation micro-CT as a novel tool to evaluate the effect of agomir-210 in a rat spinal cord injury model.
abstract: MicroRNA-210 (miR-210) was initially reported to be associated with hypoxia and plays a vital role in modulating angiogenesis. However, the potential effect and underlying mechanisms of miR-210 activity in rat spinal cord injury (SCI) have not yet been fully illuminated. In the present study, differential microRNA expression after SCI was determined by Microarray analysis. To explore the effect of miR-210 after SCI, we intrathecally injected agomir-210 with Alzet Osmotic Pumps to up-regulated the endogenous miR-210 expression. Then, synchrotron radiation micro-CT (SRμCT) imaging was used to investigate the effect of agomir-210 in rat SCI model. We found that the endogenous miR-210 expression could be up-regulated by intrathecal agomir-210 injection. The administration of agomir-210 significantly promoted angiogenesis, as evidenced by increased vessel number and volume detected by SRμCT, attenuated the lesion size and improved functional recovery after SCI. Additionally, agomir-210 attenuated cellular apoptosis and inflammation in the injured rat spinal cord. Expression levels of pro-apoptotic protein (Bax) and pro-inflammatory cytokines (TNF-α and IL-1β) were significantly decreased after agomir-210 treatment, whereas expression levels of anti-apoptotic (Bcl-2) and anti-inflammatory (IL-10) proteins were up-regulated. In conclusion, our results indicated that SRμCT is a powerful imaging tool to evaluate the effects of angiogenesis after agomir-210 administration in rat SCI model. The up-regulation of endogenous miR-210 expression following agomir-210 administration promoted angiogenesis and anti-apoptotic protein expression, and attenuated inflammation. MiR-210 played a positive role in neurological functional recovery and could be a potential new therapeutic target for SCI.
authors.affiliation: Department of Spine Surgery, Xiangya Hospital, Central South University, Changsha 410008, PR China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Cao
authors.affiliation: Department of Spine Surgery, Xiangya Hospital, Central South University, Changsha 410008, PR China.
authors.forename: Tian-Ding
authors.initials: TD
authors.lastname: Wu
authors.affiliation: Department of Spine Surgery, Xiangya Hospital, Central South University, Changsha 410008, PR China.
authors.forename: Hao
authors.initials: H
authors.lastname: Wu
authors.affiliation: Department of Spine Surgery, Xiangya Hospital, Central South University, Changsha 410008, PR China.
authors.forename: Ye
authors.initials: Y
authors.lastname: Lang
authors.affiliation: Department of Spine Surgery, Xiangya Hospital, Central South University, Changsha 410008, PR China.
authors.forename: Dong-Zhe
authors.initials: DZ
authors.lastname: Li
authors.affiliation: Department of Spine Surgery, Xiangya Hospital, Central South University, Changsha 410008, PR China.
authors.forename: Shuang-Fei
authors.initials: SF
authors.lastname: Ni
authors.affiliation: Department of Sports Medicine, Research Center of Sports Medicine, Xiangya Hospital, Central South University, Changsha 410008, PR China. Electronic address: hongbinlu@hotmail.com.
authors.forename: Hong-Bin
authors.initials: HB
authors.lastname: Lu
authors.affiliation: Department of Spine Surgery, Xiangya Hospital, Central South University, Changsha 410008, PR China. Electronic address: jianzhonghu@hotmail.com.
authors.forename: Jian-Zhong
authors.initials: JZ
authors.lastname: Hu
mesh_terms: D000792:Angiography; D000818:Animals; D017209:Apoptosis / Q000187:drug effects / Q000502:physiology; D004195:Disease Models, Animal; D004353:Drug Evaluation, Preclinical; D021621:Imaging, Three-Dimensional; D007278:Injections, Spinal; D008297:Male; D035683:MicroRNAs / Q000378:metabolism / Q000494:pharmacology*; D055806:Microvessels / Q000000981:diagnostic imaging / Q000187:drug effects / Q000503:physiopathology; D015213:Neuroimmunomodulation / Q000187:drug effects / Q000502:physiology; D018696:Neuroprotective Agents / Q000494:pharmacology*; D011897:Random Allocation; D017207:Rats, Sprague-Dawley; D020127:Recovery of Function / Q000187:drug effects / Q000502:physiology; D013116:Spinal Cord / Q000000981:diagnostic imaging / Q000187:drug effects / Q000473:pathology / Q000503:physiopathology; D013119:Spinal Cord Injuries / Q000000981:diagnostic imaging* / Q000188:drug therapy* / Q000473:pathology / Q000503:physiopathology; D017356:Synchrotrons; D016896:Treatment Outcome; D055114:X-Ray Microtomography
chemical_list: C546388:MIRN210 microRNA, rat; D035683:MicroRNAs; D018696:Neuroprotective Agents
keywords: Angiogenesis; Apoptotic; Inflammation; MiR-210; SRμCT; Spinal cord injury