title: Impact of plant growth-promoting rhizobacteria on root colonization potential and life cycle of Rhizophagus irregularis following co-entrapment into alginate beads.
abstract: OBJECTIVE
  This study aimed at evaluating the impact of seven plant growth-promoting rhizobacteria (PGPR) on root colonization and life cycle of Rhizophagus irregularis MUCL 41833 when co-entrapped in alginate beads.
  
  
  RESULTS
  Two in vitro experiments were conducted. The first consisted of the immobilization of R. irregularis and seven PGPR isolates into alginate beads to assess the effect of the bacteria on the pre-symbiotic growth of the fungus. In the second experiment, the best performing PGPR from experiment 1 was tested for its ability to promote the symbiotic development of the AMF in potato plantlets from three cultivars. Results showed that only one isolate identified as Pseudomonas plecoglossicida (R-67094) promoted germ tube elongation and hyphal branching of germinated spores during the pre-symbiotic phase of the fungus. This PGPR further promoted the symbiotic development of the AMF in potato plants.
  
  
  CONCLUSIONS
  The co-entrapment of Ps. plecoglossicida R-67094 and R. irregularis MUCL 41833 in alginate beads improved root colonization by the AMF and its further life cycle under the experimental conditions.
  
  
  CONCLUSIONS
  Co-entrapment of suitable AMF-PGPR combinations within alginate beads may represent an innovative technology that can be fine-tuned for the development of efficient consortia-based bioformulations.
authors.affiliation: Earth and Life Institute, Applied Microbiology, Mycology, Université catholique de Louvain Croix du Sud, Louvain-la-Neuve, Belgium.|Departamento de Ciencias Naturales, Universidad Técnica Particular de Loja, Loja, Ecuador.
authors.forename: P
authors.initials: P
authors.lastname: Loján
authors.affiliation: Earth and Life Institute, Applied Microbiology, Mycology, Université catholique de Louvain Croix du Sud, Louvain-la-Neuve, Belgium.
authors.forename: M
authors.initials: M
authors.lastname: Demortier
authors.affiliation: School of Biological, Earth and Environmental Sciences, University College Cork, Cork, Ireland.
authors.forename: S L S
authors.initials: SL
authors.lastname: Velivelli
authors.affiliation: Bioresources Unit, Department of Health and Environment, AIT Austrian Institute of Technology GmbH, Tulln, Austria.
authors.forename: S
authors.initials: S
authors.lastname: Pfeiffer
authors.affiliation: Departamento de Ciencias Naturales, Universidad Técnica Particular de Loja, Loja, Ecuador.
authors.forename: J P
authors.initials: JP
authors.lastname: Suárez
authors.affiliation: Laboratory for Microbiology, University of Gent, Gent, Belgium.
authors.forename: P
authors.initials: P
authors.lastname: de Vos
authors.affiliation: School of Biological, Earth and Environmental Sciences, University College Cork, Cork, Ireland.
authors.forename: B D
authors.initials: BD
authors.lastname: Prestwich
authors.affiliation: Bioresources Unit, Department of Health and Environment, AIT Austrian Institute of Technology GmbH, Tulln, Austria.
authors.forename: A
authors.initials: A
authors.lastname: Sessitsch
authors.affiliation: Earth and Life Institute, Applied Microbiology, Mycology, Université catholique de Louvain Croix du Sud, Louvain-la-Neuve, Belgium.
authors.forename: S
authors.initials: S
authors.lastname: Declerck
mesh_terms: D000464:Alginates; D001407:Bacillus / Q000145:classification / Q000502:physiology; D020563:Gammaproteobacteria / Q000145:classification / Q000502:physiology; D055137:Glomeromycota / Q000145:classification / Q000254:growth & development*; D020723:Glucuronic Acid; D006603:Hexuronic Acids; D025301:Hyphae / Q000378:metabolism; D008828:Microbiological Techniques / Q000379:methods*; D063245:Plant Development; D018517:Plant Roots / Q000382:microbiology*; D012988:Soil Microbiology; D011198:Solanum tuberosum / Q000382:microbiology
chemical_list: D000464:Alginates; D006603:Hexuronic Acids; D020723:Glucuronic Acid
keywords: 
  AMF
  ; 
  PGPR
  ; 
  Pseudomonas
  ; 
  Rhizophagus irregularis
  ; alginate beads; co-entrapment; formulation; interaction; microbial inoculant; potato