title: Identification of genes for normalization of real-time RT-PCR data in placental tissues from intrahepatic cholestasis of pregnancy.
abstract: The selection of suitable reference genes is crucial for proper interpretation of RT-qPCR data. To date, a systematic screening for reference genes in placental tissues from intrahepatic cholestasis of pregnancy (ICP) is missing. Eighteen candidate reference genes were investigated to determine their applicability by descriptive statistics and published algorithms. HPRT1, YWHAZ and PUM1 were found to be the most stable genes and could be used for normalization in gene expression study of placentas from ICP. The combination of HPRT1 and YWHAZ is sufficient and the use of all three genes should be recommended for an improved reliability of normalization.
authors.affiliation: Department of Obstetrics and Gynecology, Shanghai General Hospital, Shanghai Jiao Tong University, School of Medicine, China.
authors.forename: Yanli
authors.initials: Y
authors.lastname: Li
authors.affiliation: Department of Obstetrics and Gynecology, Shanghai General Hospital, Shanghai Jiao Tong University, School of Medicine, China.
authors.forename: Huifang
authors.initials: H
authors.lastname: Lu
authors.affiliation: Department of Obstetrics and Gynecology, Shanghai General Hospital, Shanghai Jiao Tong University, School of Medicine, China.
authors.forename: Yizhen
authors.initials: Y
authors.lastname: Ji
authors.affiliation: Department of Obstetrics and Gynecology, Shanghai General Hospital, Shanghai Jiao Tong University, School of Medicine, China. Electronic address: wusf2016@126.com.
authors.forename: Sufang
authors.initials: S
authors.lastname: Wu
authors.affiliation: Department of Obstetrics and Gynecology, Shanghai General Hospital, Shanghai Jiao Tong University, School of Medicine, China. Electronic address: ybin_yang@126.com.
authors.forename: Yongbin
authors.initials: Y
authors.lastname: Yang
mesh_terms: D048948:14-3-3 Proteins / Q000235:genetics; D000328:Adult; D002780:Cholestasis, Intrahepatic / Q000235:genetics* / Q000378:metabolism; D005260:Female; D020869:Gene Expression Profiling; D020043:Genes, Essential / Q000235:genetics*; D006801:Humans; D010920:Placenta / Q000378:metabolism*; D011247:Pregnancy; D011248:Pregnancy Complications / Q000235:genetics* / Q000378:metabolism; D016601:RNA-Binding Proteins / Q000235:genetics; D012015:Reference Standards; D015203:Reproducibility of Results; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*
chemical_list: D048948:14-3-3 Proteins; C471242:PUM1 protein, human; D016601:RNA-Binding Proteins; C572235:YWHAZ protein, human
keywords: Intrahepatic cholestasis of pregnancy; Placenta; Quantitative real-time reverse transcription polymerase chain reaction; Reference gene