title: Appearance of claudin-5+leukocytes in the central nervous system during neuroinflammation: a novel role for endothelial-derived extracellular vesicles.
abstract: The mechanism of leukocyte transendothelial migration (TEM) across the highly restrictive blood-brain barrier (BBB) remains enigmatic, with paracellular TEM thought to require leukocytes to somehow navigate the obstructive endothelial tight junctions (TJs). Transient interactions between TJ proteins on the respective leukocyte and endothelial surfaces have been proposed as one mechanism for TEM. Given the expanding role of extracellular vesicles (EVs) in intercellular communication, we investigated whether EVs derived from brain microvascular endothelial cells (BMEC) of the BBB may play a role in transferring a major TJ protein, claudin-5 (CLN-5), to leukocytes as a possible basis for such a mechanism during neuroinflammation.
  
  
  
  High-resolution 3D confocal imaging was used to highlight CLN-5 immunoreactivity in the central nervous system (CNS) and on leukocytes of mice with the neuroinflammatory condition experimental autoimmune encephalomyelitis (EAE). Both Western blotting of circulating leukocytes from wild-type mice and fluorescence imaging of leukocyte-associated eGFP-CLN-5 in the blood and CNS of endothelial-targeted, Tie-2-eGFP-CLN-5 transgenic mice were used to confirm the presence of CLN-5 protein on these cells. EVs were isolated from TNF-α-stimulated BMEC cultures and blood plasma of Tie-2-eGFP-CLN-5 mice with EAE and evaluated for CLN-5 protein by Western blotting and fluorescence-activated cell sorting (FACS), respectively. Confocal imaging and FACS were used to detect binding of endothelial-derived EVs from these two sources to leukocytes in vitro. Serial electron microscopy (serial EM) and 3D contour-based surface reconstruction were employed to view EV-like structures at the leukocyte:BBB interface in situ in inflamed CNS microvessels.
  
  
  
  A subpopulation of leukocytes immunoreactive for CLN-5 on their surface was seen to infiltrate the CNS of mice with EAE and reside in close apposition to inflamed vessels. Confocal imaging of immunostained samples and Western blotting established the presence of CLN-5+leukocytes in blood as well, implying these cells are present prior to TEM. Moreover, imaging of inflamed CNS vessels and the associated perivascular cell infiltrates from Tie-2-eGFP-CLN-5 mice with EAE revealed leukocytes bearing the eGFP label, further supporting the hypothesis CLN-5 is transferred from endothelial cells to circulating leukocytes in vivo. Western blotting of BMEC-derived EVs, corresponding in size to both exosomes and microvesicles, and FACS analysis of plasma-derived EVs from Tie-2-eGFP-CLN-5 mice with EAE validated expression of CLN-5 by EVs of endothelial origin. Confocal imaging and FACS further revealed both PKH-67-labeled EVs from cultured BMECs and eGFP-CLN-5+EVs from plasma of Tie-2-eGFP-CLN-5 mice with EAE can bind to leukocytes. Lastly, serial EM and 3D contour-based surface reconstruction revealed a close association of EV-like structures between the marginating leukocytes and BMECs in situ during EAE.
  
  
  
  During neuroinflammation, CLN-5+leukocytes appear in the CNS, and both CLN-5+leukocytes and CLN-5+EVs are detected in the blood. As endothelial cells transfer CLN-5+to leukocytes in vivo, and EVs released from BMEC bind to leukocytes in vitro, EVs may serve as the vehicles to transfer CLN-5 protein at sites of leukocyte:endothelial contact along the BBB. This action may be a prelude to facilitate TEM through the formation of temporary TJ protein bridges between these two cell types.
authors.affiliation: Blood-Brain Barrier Laboratory, University of Connecticut Health Center, 263 Farmington Ave., Farmington, CT, 06070, USA.|Department of Cell Biology, University of Connecticut Health Center, 263 Farmington Ave., Farmington, CT, 06070, USA.
authors.forename: Debayon
authors.initials: D
authors.lastname: Paul
authors.affiliation: Department of Cell Biology, University of Connecticut Health Center, 263 Farmington Ave., Farmington, CT, 06070, USA.
authors.forename: Valentina
authors.initials: V
authors.lastname: Baena
authors.affiliation: Blood-Brain Barrier Laboratory, University of Connecticut Health Center, 263 Farmington Ave., Farmington, CT, 06070, USA.|Department of Cell Biology, University of Connecticut Health Center, 263 Farmington Ave., Farmington, CT, 06070, USA.
authors.forename: Shujun
authors.initials: S
authors.lastname: Ge
authors.affiliation: Blood-Brain Barrier Laboratory, University of Connecticut Health Center, 263 Farmington Ave., Farmington, CT, 06070, USA.|Department of Cell Biology, University of Connecticut Health Center, 263 Farmington Ave., Farmington, CT, 06070, USA.
authors.forename: Xi
authors.initials: X
authors.lastname: Jiang
authors.affiliation: Department of Immunology, University of Connecticut Health Center, 263 Farmington Ave., Farmington, CT, 06070, USA.
authors.forename: Evan R
authors.initials: ER
authors.lastname: Jellison
authors.affiliation: Department of Neuroscience, University of Connecticut Health Center, 263 Farmington Ave., Farmington, CT, 06070, USA.
authors.forename: Timothy
authors.initials: T
authors.lastname: Kiprono
authors.affiliation: Department of Pathology and Cell Biology, Columbia University School of Medicine, 630 W 168th St, New York, NY, 10032, USA.
authors.forename: Dritan
authors.initials: D
authors.lastname: Agalliu
authors.affiliation: Blood-Brain Barrier Laboratory, University of Connecticut Health Center, 263 Farmington Ave., Farmington, CT, 06070, USA. pachter@uchc.edu.|Department of Cell Biology, University of Connecticut Health Center, 263 Farmington Ave., Farmington, CT, 06070, USA. pachter@uchc.edu.
authors.forename: Joel S
authors.initials: JS
authors.lastname: Pachter
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D002490:Central Nervous System / Q000000981:diagnostic imaging / Q000473:pathology*; D016207:Cytokines / Q000378:metabolism; D004195:Disease Models, Animal; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000097:blood / Q000276:immunology / Q000473:pathology*; D042783:Endothelial Cells / Q000473:pathology* / Q000648:ultrastructure; D004730:Endothelium, Vascular / Q000378:metabolism* / Q000648:ultrastructure; D000067128:Extracellular Vesicles / Q000378:metabolism* / Q000648:ultrastructure; D005260:Female; D007962:Leukocytes / Q000378:metabolism; D051907:Lysosomal Membrane Proteins; D008562:Membrane Glycoproteins / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D063308:Myelin-Oligodendrocyte Glycoprotein / Q000276:immunology / Q000633:toxicity; D010446:Peptide Fragments / Q000276:immunology / Q000633:toxicity
chemical_list: C488572:Cln5 protein, mouse; D016207:Cytokines; D051907:Lysosomal Membrane Proteins; D008562:Membrane Glycoproteins; D063308:Myelin-Oligodendrocyte Glycoprotein; D010446:Peptide Fragments; C445081:myelin oligodendrocyte glycoprotein (35-55)
keywords: Blood-brain barrier; Exosomes; Extracellular vesicles; Leukocytes; Microvesicles; Transendothelial migration