title: Efficient production of recombinant glycoprotein D of herpes simplex virus type 2 in Pichia pastoris and its protective efficacy against viral challenge in mice.
abstract: Herpes simplex virus type 2 (HSV-2) infection is the leading cause of genital ulcer disease and a significant public health concern. However, there are no approved vaccines available to prevent HSV-2 infection. The glycoprotein D (gD) of HSV-2 is the most important candidate antigen for vaccine development. In this study, a truncated form of gD (codons 1-340, gD1-340) was produced as a secretory protein in the methylotrophic yeast Pichia pastoris. The recombinant gD1-340with a His6tag was purified to homogeneity by one-step affinity chromatography. Mice immunized with the recombinant gD1-340developed high levels of antigen-specific antibody responses with HSV-2 neutralizing activity. Immunization with the recombinant gD1-340conferred significant protection against lethal HSV-2 infection in mice. Moreover, measurement of the secretion of gD1-340-specific cytokines demonstrated that the recombinant gD1-340induced mixed Th1/Th2 cellular immune responses. These findings indicated that P. pastoris-derived gD1-340represents a promising HSV-2 vaccine candidate with strong immunogenicity and prophylactic efficacy.
authors.affiliation: Institute for Translational Medicine, Medical College of Qingdao University, Qingdao, 266021, China. wangman@qdu.edu.cn.
authors.forename: Man
authors.initials: M
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan, 430072, China.
authors.forename: Shuai
authors.initials: S
authors.lastname: Jiang
authors.affiliation: Animal Biosafety Level III Laboratory at the Center for Animal Experiment, Wuhan University, Wuhan, 430071, China.
authors.forename: Li
authors.initials: L
authors.lastname: Zhou
authors.affiliation: Institute for Translational Medicine, Medical College of Qingdao University, Qingdao, 266021, China.
authors.forename: Chaoqun
authors.initials: C
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan, 430072, China.
authors.forename: Ruifeng
authors.initials: R
authors.lastname: Mao
authors.affiliation: Institute for Translational Medicine, Medical College of Qingdao University, Qingdao, 266021, China.
authors.forename: Murugavel
authors.initials: M
authors.lastname: Ponnusamy
mesh_terms: D000818:Animals; D057134:Antibodies, Neutralizing / Q000276:immunology; D000914:Antibodies, Viral / Q000276:immunology; D000917:Antibody Formation; D005260:Female; D015870:Gene Expression; D006561:Herpes Simplex / Q000276:immunology / Q000517:prevention & control* / Q000821:virology; D018258:Herpesvirus 2, Human / Q000235:genetics / Q000276:immunology*; D006801:Humans; D007114:Immunization; D051379:Mice; D008807:Mice, Inbred BALB C; D010843:Pichia / Q000235:genetics* / Q000378:metabolism; D011994:Recombinant Proteins / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology; D014759:Viral Envelope Proteins / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*
chemical_list: D057134:Antibodies, Neutralizing; D000914:Antibodies, Viral; D011994:Recombinant Proteins; D014759:Viral Envelope Proteins; C040570:glycoprotein D-herpes simplex virus type 2