title: Cryopreservation of Human Pluripotent Stem Cell-Derived Cardiomyocytes: Strategies, Challenges, and Future Directions.
abstract: In recent years, human pluripotent stem cell-derived cardiomyocytes (hPSC-CMs) have emerged as a vital cell source for in vitro modeling of genetic cardiovascular disorders, drug screening, and in vivo cardiac regeneration research. Looking forward, the ability to efficiently cryopreserve hPSC-CMs without compromising their normal biochemical and physiologic functions will dramatically facilitate their various biomedical applications. Although working protocols for freezing, storing, and thawing hPSC-CMs have been established, the question remains as to whether they are optimal. In this chapter, we discuss our current understanding of cryopreservation appertaining to hPSC-CMs, and proffer key questions regarding the mechanical, contractile, and regenerative properties of cryopreserved hPSC-CMs.
authors.affiliation: Division of Pediatric Cardiology, Department of Pediatrics, Emory University School of Medicine and Children's Healthcare of Atlanta, 2015 Uppergate Drive, Atlanta, GA, 30322, USA.|Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, GA, USA.
authors.forename: Marcela K
authors.initials: MK
authors.lastname: Preininger
authors.affiliation: Division of Pediatric Cardiology, Department of Pediatrics, Emory University School of Medicine and Children's Healthcare of Atlanta, 2015 Uppergate Drive, Atlanta, GA, 30322, USA.
authors.forename: Monalisa
authors.initials: M
authors.lastname: Singh
authors.affiliation: Division of Pediatric Cardiology, Department of Pediatrics, Emory University School of Medicine and Children's Healthcare of Atlanta, 2015 Uppergate Drive, Atlanta, GA, 30322, USA. chunhui.xu@emory.edu.|Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, GA, USA. chunhui.xu@emory.edu.
authors.forename: Chunhui
authors.initials: C
authors.lastname: Xu
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D015925:Cryopreservation / Q000295:instrumentation / Q000379:methods*; D003451:Cryoprotective Agents / Q000494:pharmacology; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D004195:Disease Models, Animal; D055808:Drug Discovery; D015507:Drugs, Investigational / Q000494:pharmacology; D006801:Humans; D051379:Mice; D017566:Microvascular Angina / Q000473:pathology / Q000628:therapy*; D017202:Myocardial Ischemia / Q000473:pathology / Q000628:therapy*; D009206:Myocardium / Q000473:pathology; D032383:Myocytes, Cardiac / Q000166:cytology* / Q000187:drug effects / Q000502:physiology / Q000637:transplantation; D039904:Pluripotent Stem Cells / Q000166:cytology* / Q000502:physiology; D012038:Regeneration / Q000502:physiology
chemical_list: D003451:Cryoprotective Agents; D015507:Drugs, Investigational; D004121:Dimethyl Sulfoxide
keywords: Beating; Cell viability; Contraction; Cryopreservation; Electrical coupling; Engraftment; Freezing; Human pluripotent stem cell-derived cardiomyocytes; Thawing; Transplantation