title: Antianxiety activity guided isolation and characterization of bergenin from Caesalpinia digyna Rottler roots.
abstract: BACKGROUND
  Caesalpinia digyna Rottler (Caesalpiniaceae) roots have been used traditionally for soothing nerves, as nervine tonic and febrifuge. The aim of the present study was to isolate the antianxiety constituent(s) from C. digyna roots following bioactivity guided fractionation approach.
  
  
  METHODS
  Bioactive fraction F4 was subjected to column chromatography to get three sub-fractions (F4.1-F4.3) and a compound CD2. CD1 and CD2 were characterized using standard spectral techniques. Three fractions (F4.1-F4.3) were evaluated for antianxiety activity in mice using EPM. An effective dose of the antianxiety principle was optimized, and its activity was, further evaluated using open field and mirror chamber tests.
  
  
  RESULTS
  CD1 and CD2 characterized as bergenin, exhibited significant antianxiety activity at 80mg/kg, po which was statistically comparable to that of diazepam (2mg/kg, po). However, F4.1-F4.3 were observed to be devoid of antianxiety activity. Bergenin also exhibited significant antianxiety activity in open field and mirrored chamber tests.
  
  
  CONCLUSIONS
  Bergenin, isolated from roots of C. digyna, exhibits significant antianxiety activity at 80mg/kg, po in three different models for evaluating antianxiety activity. This activity of bergenin is being reported for the first time.
authors.affiliation: University Institute of Pharmaceutical Sciences, Panjab University, Chandigarh 160014, India.
authors.forename: Jitender
authors.initials: J
authors.lastname: Singh
authors.affiliation: University Institute of Pharmaceutical Sciences, Panjab University, Chandigarh 160014, India. Electronic address: bashwani@pu.ac.in.
authors.forename: Ashwani
authors.initials: A
authors.lastname: Kumar
authors.affiliation: University Institute of Pharmaceutical Sciences, Panjab University, Chandigarh 160014, India.
authors.forename: Anupam
authors.initials: A
authors.lastname: Sharma
mesh_terms: D000818:Animals; D014151:Anti-Anxiety Agents / Q000302:isolation & purification / Q000494:pharmacology*; D001007:Anxiety / Q000517:prevention & control* / Q000523:psychology; D001522:Behavior, Animal / Q000187:drug effects*; D001578:Benzopyrans / Q000302:isolation & purification / Q000494:pharmacology*; D029911:Caesalpinia / Q000737:chemistry*; D005591:Chemical Fractionation; D002850:Chromatography, Gel; D003975:Diazepam / Q000494:pharmacology; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D005260:Female; D008297:Male; D051379:Mice; D009043:Motor Activity / Q000187:drug effects*; D008517:Phytotherapy; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology*; D018517:Plant Roots / Q000737:chemistry*; D010946:Plants, Medicinal
chemical_list: D014151:Anti-Anxiety Agents; D001578:Benzopyrans; D010936:Plant Extracts; C006741:bergenin; D003975:Diazepam
keywords: Antianxiety; Antianxiety activity-guided fractionation; Bergenin; Bergenin, PubChem (CID 66065); Caesalpinia digyna; Diazepam, PubChem (CID 3016)