title: Composition and diversity of mucosa-associated microbiota along the entire length of the pig gastrointestinal tract; dietary influences.
abstract: Mucosa-associated microbial populations of the gastrointestinal tract are in intimate contact with the outer mucus layer. This proximity offers these populations a higher potential, than lumenal microbiota, in exerting effects on the host. Functional characteristics of the microbiota and influences of host-physiology shape the composition and activity of the mucosa-associated bacterial community. We have shown previously that inclusion of an artificial sweetener, SUCRAM, included in the diet of weaning piglets modulates the composition of lumenal-residing gut microbiota and reduces weaning-related gastrointestinal disorders. In this study, using Illumina sequencing we characterised the mucosa-associated microbiota along the length of the intestine of piglets, and determined the effect of SUCRAM supplementation on mucosa-associated populations. There were clear distinctions in the composition of mucosa-associated microbiota, between small and large intestine, concordant with differences in regional oxygen distribution and nutrient provision by the host. There were significant differences in the composition of mucosa-associated compared with lumenal microbiota in pig caecum. Dietary supplementation with SUCRAM affected mucosa-associated bacterial community structure along the length of the intestinal tract. Most notably, there was a substantial reduction in predominant Campylobacter populations proposing that SUCRAM supplementation of swine diet has potential for reducing meat contamination and promoting food safety.
authors.affiliation: Department of Functional and Comparative Genomics, Institute of Integrative Biology, University of Liverpool, Liverpool, L69 7ZB, UK.
authors.forename: Jennifer
authors.initials: J
authors.lastname: Kelly
authors.affiliation: Department of Functional and Comparative Genomics, Institute of Integrative Biology, University of Liverpool, Liverpool, L69 7ZB, UK.
authors.forename: Kristian
authors.initials: K
authors.lastname: Daly
authors.affiliation: Department of Functional and Comparative Genomics, Institute of Integrative Biology, University of Liverpool, Liverpool, L69 7ZB, UK.
authors.forename: Andrew W
authors.initials: AW
authors.lastname: Moran
authors.affiliation: Department of Functional and Comparative Genomics, Institute of Integrative Biology, University of Liverpool, Liverpool, L69 7ZB, UK.
authors.forename: Sheila
authors.initials: S
authors.lastname: Ryan
authors.affiliation: Pancosma SA, Voie-des-Traz 6, Le Grand-Sacconex, Geneva, CH 1218, Switzerland.
authors.forename: David
authors.initials: D
authors.lastname: Bravo
authors.affiliation: Department of Functional and Comparative Genomics, Institute of Integrative Biology, University of Liverpool, Liverpool, L69 7ZB, UK.
authors.forename: Soraya P
authors.initials: SP
authors.lastname: Shirazi-Beechey
mesh_terms: D000818:Animals; D004032:Diet; D019587:Dietary Supplements; D000069196:Gastrointestinal Microbiome; D041981:Gastrointestinal Tract / Q000382:microbiology; D007413:Intestinal Mucosa / Q000382:microbiology*; D013552:Swine / Q000382:microbiology*; D014886:Weaning