title: Production of monoclonal antibody against recombinant NS3 protein of bovine viral diarrhea virus (NADL strain).
abstract: This study was conducted to investigate the prevalence of subclinical mastitis caused byStaphylococcusspp. in ewes in West-Azerbaijan province of Iran. Molecular characterization of isolatedStaphylococcusspp.from diseased ewes were performed using polymerase chain reaction (PCR) followed by restriction fragment length polymorphism (RFLP) and DNA sequencing of glyceraldehyde-3-phosphate dehydrogenase (gap) gene. Also, antibiotic resistance of staphylococcal isolates against different antibiotics was investigated. A total number of 900 milk samples from 450 native ewes in their mid-lactation period were examined by the California mastitis test (CMT). The CMT positive samples were cultured and bacteria were isolated from 86 (9.50%) glands and 74 (16.40%) ewes. The prevalence of subclinical mastitis in the examined ewes was 16.40%. Microbiological analysis of milk samples revealed that 27 out of 74 sheep with subclinical mastitis were infected withStaphylococcusspp. Amplification ofgapgene of 27Staphylococcusisolates generated a single amplicon of 933 bp in size confirming that isolates were belonged toStaphylococcusgenus. Digestion of PCR products byAluI endonuclease generated different RFLP patterns for each species. Nucleotide sequencing ofgapgene followed by phylogenetic analysis showed that the most dominantStaphylococcusspecies wereS. epidermidis,S. xylosusandS. chromogenes. Staphylococcal isolates showed the highest resistance to penicillin and ampicillin. In conclusion,Staphylococcusspecies, except for the southern parts of the province, play an important role in the development of subclinical mastitis in sheep in West-Azerbaijan province of Iran. Also, chloramphenicol, ciprofloxacin and neomycin are the most effective antibiotics for treatment of this disease.
authors.affiliation: Department of Pathobiology, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran.
authors.forename: Masood Reza
authors.initials: MR
authors.lastname: Seyfi Abad Shapouri
authors.affiliation: Department of Pharmacognosy, Faculty of Pharmacy, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
authors.forename: Maryam
authors.initials: M
authors.lastname: Ekhtelat
authors.affiliation: Department of Pathobiology, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran.
authors.forename: Masood
authors.initials: M
authors.lastname: Ghorbanpoor Najaf Abadi
authors.affiliation: Department of Quality control, Razi Vaccine and Serum Research Institute, Karaj, Iran.
authors.forename: Mohsen
authors.initials: M
authors.lastname: Lotfi
authors.affiliation: Department of Immunology, Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
authors.forename: Mohamad
authors.initials: M
authors.lastname: Rashno
keywords: Bovine viral diarrhea; Monoclonal antibody; NS3; Recombinant antigen