title: Application ofmyostatinin sheep breeding programs: A review.
abstract: Plasma membrane H+-ATPase is a major integral membrane protein with a role in various physiological processes including abiotic stress response. To study the effect of NaCl on the expression pattern of a gene encoding the plasma membrane H+-ATPase, an experiment was carried out in a completely random design with three replications. A pair of specific primers was designed based on the sequence of the gene encoding plasma membrane H+-ATPase inAeluropus littoralisto amplify a 259 bp fragment from the target gene by PCR. A gene encoding actin was used as reference gene to normalize the expression level of the target gene. A pair of specific primers was designed to amplify a 157 bp fragment from the actin gene by PCR. Plants were treated with different concentrations of NaCl, 0, 50, 100, 150, 200, 250, 500 and 1000 mM, for two days. Our results showed that the expression level of the plasma membrane H+-ATPase gene increased dramatically at 500 mM and then decreased with increasing concentrations of NaCl. The results also indicated that the leaves of plants, were treated with high concentrations of NaCl changed morphologically, but those grown under low concentrations of NaCl as well as the control plants did not show morphological changes in their leaves. Our results suggest a relation between morphological changes of treated plants and the expression level of the plasma membrane H+-ATPase gene inAeluropus littoralis.
authors.affiliation: Department of Animal and Poultry Science, College of Abouraihan, University of Tehran, Iran ; Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, Alberta, Canada.
authors.forename: Younes
authors.initials: Y
authors.lastname: Miar
authors.affiliation: Department of Animal and Poultry Science, College of Abouraihan, University of Tehran, Iran.
authors.forename: Abdolreza
authors.initials: A
authors.lastname: Salehi
authors.affiliation: Monsanto Company, 3302 SE Convenience Blvd, Ankeny, Iowa, 50021, USA.
authors.forename: Davood
authors.initials: D
authors.lastname: Kolbehdari
authors.affiliation: National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran.
authors.forename: Seyed Ahmad
authors.initials: SA
authors.lastname: Aleyasin
keywords: NaCl; Real-time PCR; gene expression; gouan; proton pump