title: A modification of intraluminal middle cerebral artery occlusion/reperfusion model for ischemic stroke with laser Doppler flowmetry guidance in mice.
abstract: Stroke is one of the common causes of death and disability in the world. The intraluminal middle cerebral artery occlusion/reperfusion (MCAO/R) model is a "gold standard" in surgical ischemic stroke models. Here, we optimized the procedure of this model by ligating on external carotid artery (ECA) stump and two ligatures prepared on internal carotid artery, which could improve the success and survival rate in mice. The results show that ECA approach was superior to common carotid artery approach. Meanwhile, we found that the exposure of pterygopalatine artery was not an essential step for MCAO/R model in mice.
authors.affiliation: Department of Neurosurgery, Renmin Hospital of Wuhan University, Wuhan.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Cai
authors.affiliation: Department of Neurosurgery, Xiantao First People's Hospital, Xiantao.
authors.forename: Gang
authors.initials: G
authors.lastname: Xu
authors.affiliation: Department of Neurosurgery, Renmin Hospital of Wuhan University, Wuhan.
authors.forename: Junhui
authors.initials: J
authors.lastname: Liu
authors.affiliation: Department of Neurosurgery, Renmin Hospital of Wuhan University, Wuhan.
authors.forename: Long
authors.initials: L
authors.lastname: Wang
authors.affiliation: Department of Neurosurgery, Renmin Hospital of Wuhan University, Wuhan.
authors.forename: Gang
authors.initials: G
authors.lastname: Deng
authors.affiliation: Department of Emergency, The Central Hospital of Wuhan, Wuhan, Hubei, People's Republic of China.
authors.forename: Jun
authors.initials: J
authors.lastname: Liu
authors.affiliation: Department of Neurosurgery, Renmin Hospital of Wuhan University, Wuhan.
authors.forename: Zhibiao
authors.initials: Z
authors.lastname: Chen
keywords: animal model; ischemia stroke; mice; middle cerebral artery occlusion