title: Artemisia capillaris Alleviates Bone Loss by Stimulating Osteoblast Mineralization and Suppressing Osteoclast Differentiation and Bone Resorption.
abstract: Artemisia capillaris has been used to treat jaundice and relieve high liver-heat in traditional medicine. In this study, we found that the administration of a water extract from A. capillaris (WEAC) to the receptor activator of nuclear factor kappa-B ligand (RANKL)-induced bone loss model significantly prevents osteoporotic bone loss, increasing bone volume/trabecular volume by 22% and trabecular number by 24%, and decreasing trabecular separation by 29%. WEAC stimulated in vitro osteoblast mineralization from primary osteoblasts in association with increasing expression of osterix, nuclear factor of activated T cells cytoplasmic 1, and activator protein-1, as well as phosphorylation of extracellular signal-regulated kinase. In contrast to the anabolic effect of WEAC, WEAC significantly suppressed in vitro osteoclast formation from bone marrow macrophages by inhibiting the RANKL signaling pathways and bone resorption by downregulating the expression of resorption markers. Therefore, this study demonstrated that WEAC has a beneficial effect on bone loss through the regulation of osteoblast mineralization, as well as osteoclast formation and bone resorption. These results suggest that A. capillaris may be a promising herbal candidate for therapeutic agents to treat or prevent osteoporotic bone diseases.
authors.affiliation: 1 KM Application Center, Korea Institute of Oriental Medicine, Dong-gu, Daegu 701-300, Republic of Korea.
authors.forename: Chung-Jo
authors.initials: CJ
authors.lastname: Lee
authors.affiliation: 1 KM Application Center, Korea Institute of Oriental Medicine, Dong-gu, Daegu 701-300, Republic of Korea.
authors.forename: Ki-Shuk
authors.initials: KS
authors.lastname: Shim
authors.affiliation: 1 KM Application Center, Korea Institute of Oriental Medicine, Dong-gu, Daegu 701-300, Republic of Korea.
authors.forename: Jin Yeul
authors.initials: JY
authors.lastname: Ma
mesh_terms: D000818:Animals; D019306:Artemisia / Q000737:chemistry*; D001862:Bone Resorption / Q000188:drug therapy*; D002113:Calcification, Physiologic / Q000187:drug effects*; D002454:Cell Differentiation / Q000187:drug effects*; D002478:Cells, Cultured; D003864:Depression, Chemical; D004195:Disease Models, Animal; D008297:Male; D008813:Mice, Inbred ICR; D010006:Osteoblasts / Q000378:metabolism*; D010010:Osteoclasts / Q000166:cytology*; D010024:Osteoporosis / Q000188:drug therapy* / Q000378:metabolism / Q000517:prevention & control; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology* / Q000627:therapeutic use; D013268:Stimulation, Chemical; D014867:Water
chemical_list: D010936:Plant Extracts; D014867:Water
keywords: Artemisia capillaris; Nuclear Factor of Activated T cells Cytoplasmic 1; Osteoblast; Osteoclast; Osterix; Receptor Activator of Nuclear Factor Kappa-B Ligand