title: The Molecular Blueprint of a Fungus by Next-Generation Sequencing (NGS).
abstract: Sequencing the whole genome of an organism is invaluable for its comprehensive molecular characterization and has been drastically facilitated by the advent of high-throughput sequencing techniques. Especially in clinical microbiology the impact of sequenced strains increases as resistance and virulence markers can easily be detected. Here, we describe a combined approach for sequencing a fungal genome and transcriptome from initial nucleic acid isolation through the generation of ready-to-load DNA libraries for the Illumina platform and the final step of genome assembly with subsequent gene annotation.
authors.affiliation: Fraunhofer Institute for Interfacial Engineering and Biotechnology, Stuttgart, Germany.
authors.forename: Christian
authors.initials: C
authors.lastname: Grumaz
authors.affiliation: Fraunhofer Institute for Interfacial Engineering and Biotechnology, Stuttgart, Germany.
authors.forename: Philipp
authors.initials: P
authors.lastname: Kirstahler
authors.affiliation: Fraunhofer Institute for Interfacial Engineering and Biotechnology, Stuttgart, Germany. kai.sohn@igb.fraunhofer.de.|Institute of Interfacial Process Engineering and Plasma Technology, University of Stuttgart, Stuttgart, Germany. kai.sohn@igb.fraunhofer.de.
authors.forename: Kai
authors.initials: K
authors.lastname: Sohn
mesh_terms: D001483:Base Sequence; D016681:Genome, Fungal; D059014:High-Throughput Nucleotide Sequencing; D006801:Humans; D058977:Molecular Sequence Annotation; D009181:Mycoses / Q000175:diagnosis* / Q000382:microbiology; D012331:RNA, Fungal / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D017422:Sequence Analysis, DNA; D017423:Sequence Analysis, RNA; D059467:Transcriptome
chemical_list: D012331:RNA, Fungal; D012333:RNA, Messenger
keywords: Annotation; Assembly; Bioinformatics; Fungal genome; Next-generation sequencing (NGS); RNA sequencing (RNA-seq); Whole-genome shotgun sequencing (WGS)