title: Transcriptome analysis revealed the dynamic oil accumulation in Symplocos paniculata fruit.
abstract: Symplocos paniculata, asiatic sweetleaf or sapphire berry, is a widespread shrub or small tree from Symplocaceae with high oil content and excellent fatty acid composition in fruit. It has been used as feedstocks for biodiesel and cooking oil production in China. Little transcriptome information is available on the regulatory molecular mechanism of oil accumulation at different fruit development stages.
  
  
  
  The transcriptome at four different stages of fruit development (10, 80,140, and 170 days after flowering) of S. paniculata were analyzed. Approximately 28 million high quality clean reads were generated. These reads were trimmed and assembled into 182,904 non-redundant putative transcripts with a mean length of 592.91 bp and N50 length of 785 bp, respectively. Based on the functional annotation through Basic Local Alignment Search Tool (BLAST) with public protein database, the key enzymes involved in lipid metabolism were identified, and a schematic diagram of the pathway and temporal expression patterns of lipid metabolism was established. About 13,939 differentially expressed unigenes (DEGs) were screened out using differentially expressed sequencing (DESeq) method. The transcriptional regulatory patterns of the identified enzymes were highly related to the dynamic oil accumulation along with the fruit development of S. paniculata. In addition, quantitative real-time PCR (qRT-PCR) of six vital genes was significantly correlated with DESeq data.
  
  
  
  The transcriptome sequences obtained and deposited in NCBI would enrich the public database and provide an unprecedented resource for the discovery of the genes associated with lipid metabolism pathway in S. paniculata. Results in this study will lay the foundation for exploring transcriptional regulatory profiles, elucidating molecular regulatory mechanisms, and accelerating genetic engineering process to improve the yield and quality of seed oil of S. paniculata.
authors.affiliation: Central South University of Forestry and Technology, 498 South Shaoshan Rd., Changsha, Hunan, 410004, China.|Texas A&M AgriLife Research Center at El Paso, 1380 A&M Circle, El Paso, TX, 79927, USA.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Liu
authors.affiliation: Texas A&M AgriLife Research Center at El Paso, 1380 A&M Circle, El Paso, TX, 79927, USA.
authors.forename: Youping
authors.initials: Y
authors.lastname: Sun
authors.affiliation: Central South University of Forestry and Technology, 498 South Shaoshan Rd., Changsha, Hunan, 410004, China.|Hunan Academy of Forestry, 658 South Shaoshan Rd., Changsha, Hunan, 410004, China.
authors.forename: Jinzheng
authors.initials: J
authors.lastname: Chen
authors.affiliation: Hunan Academy of Forestry, 658 South Shaoshan Rd., Changsha, Hunan, 410004, China.
authors.forename: Peiwang
authors.initials: P
authors.lastname: Li
authors.affiliation: Hunan Academy of Forestry, 658 South Shaoshan Rd., Changsha, Hunan, 410004, China.
authors.forename: Changzhu
authors.initials: C
authors.lastname: Li
authors.affiliation: Texas A&M AgriLife Research Center at El Paso, 1380 A&M Circle, El Paso, TX, 79927, USA.
authors.forename: Genhua
authors.initials: G
authors.lastname: Niu
authors.affiliation: Central South University of Forestry and Technology, 498 South Shaoshan Rd., Changsha, Hunan, 410004, China. znljiang2542@163.com.
authors.forename: Lijuan
authors.initials: L
authors.lastname: Jiang
mesh_terms: D030541:Databases, Genetic; D004798:Enzymes / Q000235:genetics / Q000378:metabolism; D005227:Fatty Acids / Q000032:analysis / Q000096:biosynthesis; D005638:Fruit / Q000235:genetics; D008401:Gas Chromatography-Mass Spectrometry; D020869:Gene Expression Profiling; D059014:High-Throughput Nucleotide Sequencing; D019684:Magnoliopsida / Q000235:genetics*; D010938:Plant Oils / Q000032:analysis / Q000378:metabolism*; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D018749:RNA, Plant / Q000302:isolation & purification / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D059467:Transcriptome
chemical_list: D004798:Enzymes; D005227:Fatty Acids; D010938:Plant Oils; D010940:Plant Proteins; D018749:RNA, Plant
keywords: Differentially expressed profile; Oil accumulation; Symplocos paniculata; Transcriptome analysis