title: Serum Protein KNG1, APOC3, and PON1 as Potential Biomarkers for Yin-Deficiency-Heat Syndrome.
abstract: Yin-deficiency-heat (YDH) syndrome is a concept in Traditional Chinese Medicine (TCM) for describing subhealth status. However, there are few efficient diagnostic methods available for confirming YDH syndrome. To explore the novel method for diagnosing YDH syndrome, we applied iTRAQ to observe the serum protein profiles in YDH syndrome rats and confirmed protein levels by ELISA. A total of 92 differentially expressed proteins (63 upregulated proteins and 29 downregulated proteins), which were mainly involved in complement and coagulation cascades and glucose metabolism pathway, were identified by the proteomic experiments. Kininogen 1 (KNG1) was significantly increased (p< 0.0001), while apolipoprotein C-III (APOC3,p< 0.005) and paraoxonase 1 (PON1,p< 0.001) were significantly decreased in the serum of YDH syndrome rats. The combination of KNG1, APOC3, and PON1 constituted a diagnostic model with 100.0% sensitivity and 85.0% specificity. The results indicated that KNG1, APOC3, and PON1 may act as potential biomarkers for diagnosing YDH syndrome. KNG1 may regulate cytokines and chemokines release in YDH syndrome, and the low levels of PON1 and APOC3 may increase oxidative stress and lipolysis in YDH syndrome, respectively. Our work provides a novel method for YDH syndrome diagnosis and also provides valuable experimental basis to understand the molecular mechanism of YDH syndrome.
authors.affiliation: Institute of Cell Biology, Zhejiang University, Hangzhou 310058, China.
authors.forename: Changming
authors.initials: C
authors.lastname: Liu
authors.affiliation: Institute of Cell Biology, Zhejiang University, Hangzhou 310058, China.
authors.forename: Liangen
authors.initials: L
authors.lastname: Mao
authors.affiliation: Institute of Cell Biology, Zhejiang University, Hangzhou 310058, China.
authors.forename: Zepeng
authors.initials: Z
authors.lastname: Ping
authors.affiliation: Institute of Cell Biology, Zhejiang University, Hangzhou 310058, China.
authors.forename: Tingting
authors.initials: T
authors.lastname: Jiang
authors.affiliation: Institute of Cell Biology, Zhejiang University, Hangzhou 310058, China.
authors.forename: Chong
authors.initials: C
authors.lastname: Wang
authors.affiliation: Institute of Cell Biology, Zhejiang University, Hangzhou 310058, China.
authors.forename: Zhongliang
authors.initials: Z
authors.lastname: Chen
authors.affiliation: Institute of Cell Biology, Zhejiang University, Hangzhou 310058, China.
authors.forename: Zhongjie
authors.initials: Z
authors.lastname: Li
authors.affiliation: Institute of Cell Biology, Zhejiang University, Hangzhou 310058, China.
authors.forename: Jicheng
authors.identifier: 0000-0002-9631-8931
authors.initials: J
authors.lastname: Li