title: Targeted profiling of arachidonic acid and eicosanoids in rat tissue by UFLC-MS/MS: Application to identify potential markers for rheumatoid arthritis.
abstract: We describe a method for the targeted analysis of bioactive arachidonic acid metabolites through cyclooxygenase (COX) and lipoxygenase (LOX) pathway in knee joint, liver, kidney, spleen and heart using an ultra-fast liquid chromatography-tandem mass (UFLC-MS/MS) method. Method validation was investigated, including linearity, precision, accuracy, matrix effect, extraction recovery and stability for the simultaneous analysis of prostaglandins (PGs), thromboxanes (TXs), leukotrienes (LTs) and hydroxyeicosatetraenoic acids (HETEs). The method enables us to chromatographically separate branched-chain species from their straight-chain isomers as well as separate biologically important eicosanoids. The concentrations of the following major eicosanoids were significantly increased in rheumatoid arthritis model rats than in normal ones: 5-HETE, 8-HETE, 12-HETE, 15-HETE, PGF2α, TXB2, 5-HpETE, LTE4, PGE2, PGD2, LTB4. Further multivariate data analysis (partial least square-discriminant analysis) showed COX products (PGs, TXs) were readily distributed towards liver and kidney, LOX products (LTs, HETEs) towards knee joint and spleen, and heart had no characteristic metabolites. The method described here offers a useful tool for the evaluation of complex regulatory eicosanoids responses in RA disease states and provides support for use of dual inhibitors of COX and LOX enzymes on RA treatment.
authors.affiliation: School of Pharmacy, Shenyang Pharmaceutical University, Shenyang, China; National and Local United Engineering Laboratory for Key Technology of Chinese Material Medica Quality Control, Shenyang Pharmaceutical University, Shenyang, China.
authors.forename: Nannan
authors.initials: N
authors.lastname: Wang
authors.affiliation: School of Pharmacy, Shenyang Pharmaceutical University, Shenyang, China; National and Local United Engineering Laboratory for Key Technology of Chinese Material Medica Quality Control, Shenyang Pharmaceutical University, Shenyang, China.
authors.forename: Xiaoning
authors.initials: X
authors.lastname: Zhao
authors.affiliation: School of Pharmacy, Shenyang Pharmaceutical University, Shenyang, China; National and Local United Engineering Laboratory for Key Technology of Chinese Material Medica Quality Control, Shenyang Pharmaceutical University, Shenyang, China.
authors.forename: Weihui
authors.initials: W
authors.lastname: Wang
authors.affiliation: School of Pharmacy, Shenyang Pharmaceutical University, Shenyang, China; National and Local United Engineering Laboratory for Key Technology of Chinese Material Medica Quality Control, Shenyang Pharmaceutical University, Shenyang, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Peng
authors.affiliation: School of Pharmacy, Shenyang Pharmaceutical University, Shenyang, China; National and Local United Engineering Laboratory for Key Technology of Chinese Material Medica Quality Control, Shenyang Pharmaceutical University, Shenyang, China.
authors.forename: Kaishun
authors.initials: K
authors.lastname: Bi
authors.affiliation: School of Pharmacy, Shenyang Pharmaceutical University, Shenyang, China; National and Local United Engineering Laboratory for Key Technology of Chinese Material Medica Quality Control, Shenyang Pharmaceutical University, Shenyang, China. Electronic address: ronghuadai@sina.com.
authors.forename: Ronghua
authors.initials: R
authors.lastname: Dai
mesh_terms: D000818:Animals; D016718:Arachidonic Acid / Q000032:analysis* / Q000302:isolation & purification / Q000378:metabolism; D001172:Arthritis, Rheumatoid / Q000201:enzymology / Q000378:metabolism; D015415:Biomarkers / Q000032:analysis / Q000378:metabolism; D002853:Chromatography, Liquid / Q000379:methods*; D015777:Eicosanoids / Q000032:analysis* / Q000302:isolation & purification / Q000378:metabolism; D006893:Hydroxyeicosatetraenoic Acids / Q000032:analysis / Q000302:isolation & purification / Q000378:metabolism; D015289:Leukotrienes / Q000032:analysis / Q000302:isolation & purification / Q000378:metabolism; D008084:Lipoxygenase / Q000378:metabolism; D008297:Male; D055432:Metabolomics / Q000379:methods; D011451:Prostaglandin-Endoperoxide Synthases / Q000378:metabolism; D011453:Prostaglandins / Q000032:analysis / Q000302:isolation & purification / Q000378:metabolism; D017207:Rats, Sprague-Dawley; D053719:Tandem Mass Spectrometry / Q000379:methods*; D013931:Thromboxanes / Q000032:analysis / Q000302:isolation & purification / Q000378:metabolism
chemical_list: D015415:Biomarkers; D015777:Eicosanoids; D006893:Hydroxyeicosatetraenoic Acids; D015289:Leukotrienes; D011453:Prostaglandins; D013931:Thromboxanes; D016718:Arachidonic Acid; D008084:Lipoxygenase; D011451:Prostaglandin-Endoperoxide Synthases
keywords: Biomarker; Eicosanoids; Mechanism; Rheumatoid arthritis; UFLC–MS/MS method