title: Structural characterization and macrophage immunomodulatory activity of a novel polysaccharide from Smilax glabra Roxb.
abstract: A novel heteropolysaccharide (SGRP1) with great immunomodulatory activity was isolated from the root of Smilax glabra Roxb. by hot water extraction. Physical and chemical analyses showed that SGRP1 had an average molecular weight of 1.26×104Da and was composed of mannose, fucose and glucose in molar ratio of 1.00:3.09:39.41. The glycosidic linkage types of SGRP1 were proven to be (1→3)-linked -α-l-Fuc, (1→3)-linked-α-l-Man, (1→)-linked-α-d-Glc, and (1→6)-linked-α-d-Glc. The in vitro immunomodulatory assays demonstrated that SGRP1 could evidently promote the phagocytosis and increase macrophage-derived biological factors including nitric oxide (NO), interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β) secretion via JNK and ERK signaling pathways and NLRP3 inflammasome signaling pathway. The data supported that SGRP1 had immunomodulatory potential through activating macrophages and enhancing host immune system function, which enabled it to be a novel immunomodulator for application in immunological diseases or functional food.
authors.affiliation: The Second Institute of Clinical Medicine, Guangzhou University of Chinese Medicine, Guangzhou 510120, China.
authors.forename: Min
authors.initials: M
authors.lastname: Wang
authors.affiliation: The Second Institute of Clinical Medicine, Guangzhou University of Chinese Medicine, Guangzhou 510120, China.
authors.forename: Xiao-Bo
authors.initials: XB
authors.lastname: Yang
authors.affiliation: The Second Institute of Clinical Medicine, Guangzhou University of Chinese Medicine, Guangzhou 510120, China.
authors.forename: Jing-Wen
authors.initials: JW
authors.lastname: Zhao
authors.affiliation: The Second Institute of Clinical Medicine, Guangzhou University of Chinese Medicine, Guangzhou 510120, China. Electronic address: luchuanjian888@vip.sina.com.
authors.forename: Chuan-Jian
authors.initials: CJ
authors.lastname: Lu
authors.affiliation: The Second Institute of Clinical Medicine, Guangzhou University of Chinese Medicine, Guangzhou 510120, China. Electronic address: zhuwei160409@yahoo.com.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhu
mesh_terms: D006801:Humans; D007155:Immunologic Factors / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D015850:Interleukin-6 / Q000378:metabolism; D008264:Macrophages / Q000187:drug effects*; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D011134:Polysaccharides / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D032281:Smilax / Q000737:chemistry*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D007155:Immunologic Factors; D015850:Interleukin-6; D010936:Plant Extracts; D011134:Polysaccharides; D014409:Tumor Necrosis Factor-alpha
keywords: Immunomodulatory activity; NLRP3 inflammasome; Polysaccharide; Smilax glabra Roxb.; Structure