title: Alteration of histone H3K4 methylation in glomerular podocytes associated with proteinuria in patients with membranous nephropathy.
abstract: Histone H3K4 trimethylation (H3K4 me3) is found in active euchromatic regions and plays an important role in podocyte function in which actin filaments are abundant in the foot processes. The pathogenesis of membranous nephropathy (MN), the most prevalent cause of primary nephrotic syndrome in the middle-aged and elderly, is podocyte dysfunction.
  
  
  
  We investigated the role of H3K4 me3 in podocyte dysfunction in nephrotic syndrome using cultured podocytes and a mouse proteinuria model induced by LPS. We examined renal biopsy specimens from six patients with nephrotic syndrome caused by Phospholipase-A2-Receptor-positive primary MN.
  
  
  
  H3K4 me3 exhibited a pattern of nuclear expression in podocytes of the kidneys from patients with MN. The overlapping expression of H3K4 me3 and cathepsin L (a potent endoprotease causing the breakdown of actin-associated protein within lysosomal compartments in kidney podocytes) were higher in patients with MN compared with the controls. Histone H3K4 me3 in kidney podocytes was negatively correlated with synaptopodin, an actin-associated protein in podocytes, and the expression was positively correlated with the proteinuria levels in patients with MN. Histone H3K4 me3 levels were elevated in podocytes of LPS-treated mice, combined with an increase in podocyte swelling, an elevation of serum creatinine and urine albumin, increased cathepsin L, and decreased synaptopodin expression. Histone H3K4 me3 levels at the cathepsin L promoter were elevated in LPS-exposed mouse kidneys. The administration of shRNA against MLL3 (an H3K4 methyltransferase) to LPS-treated mice and cultured podocytes co-cultured with LPS-stimulated macrophages ameliorated podocyte swelling, an elevation in the serum creatinine and urine albumin levels and an increased expression of histone H3K4 me3 and cathepsin L, and a decreased expression of synaptopodin and increase in histone H3K4 me3 levels at the cathepsin L promoter.
  
  
  
  Histone H3K4 me3 upregulation may be involved in podocyte dysfunction and the pathophysiology of MN. Targeting this epigenetic signature of histone H3K4 me3 followed by modulating the actin dynamics may be an effective strategy to ameliorate the consequences of MN.
authors.affiliation: Department of Internal Medicine, Cardiovascular Respiratory and Neurology and Nephrology Division, Asahikawa Medical University, Midorigaoka Higashi 2-1-1-1, Asahikawa, 078-8510, Japan. ffuji42@asahikawa-med.ac.jp.
authors.forename: Takayuki
authors.identifier: 0000-0003-4530-8511
authors.initials: T
authors.lastname: Fujino
authors.affiliation: Department of Internal Medicine, Cardiovascular Respiratory and Neurology and Nephrology Division, Asahikawa Medical University, Midorigaoka Higashi 2-1-1-1, Asahikawa, 078-8510, Japan.
authors.forename: Naoyuki
authors.initials: N
authors.lastname: Hasebe
mesh_terms: D000328:Adult; D000368:Aged; D000818:Animals; D056668:Cathepsin L / Q000378:metabolism; D002478:Cells, Cultured; D019175:DNA Methylation; D015536:Down-Regulation / Q000235:genetics; D005260:Female; D015433:Glomerulonephritis, Membranous / Q000150:complications / Q000378:metabolism / Q000473:pathology*; D011495:Histone-Lysine N-Methyltransferase / Q000235:genetics; D006657:Histones / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D007678:Kidney Glomerulus / Q000473:pathology*; D008070:Lipopolysaccharides; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008840:Microfilament Proteins / Q000378:metabolism; D008875:Middle Aged; D009404:Nephrotic Syndrome / Q000209:etiology / Q000378:metabolism / Q000473:pathology*; D050199:Podocytes / Q000378:metabolism* / Q000648:ultrastructure; D011507:Proteinuria / Q000139:chemically induced / Q000378:metabolism* / Q000503:physiopathology; D034741:RNA, Small Interfering / Q000235:genetics; D015854:Up-Regulation / Q000235:genetics; D004268:DNA-Binding Proteins
chemical_list: D056668:Cathepsin L; D011495:Histone-Lysine N-Methyltransferase; D006657:Histones; D008070:Lipopolysaccharides; D008840:Microfilament Proteins; D034741:RNA, Small Interfering; C503967:SYNPO protein, human; C503968:Synpo protein, mouse; C510652:Kmt2c protein, mouse; D004268:DNA-Binding Proteins; C534281:CTSL protein, human; C534278:Ctsl protein, mouse