title: Oligoclonal expansion of TCR Vδ T cells may be a potential immune biomarker for clinical outcome of acute myeloid leukemia.
abstract: Recent data have shown that γδ T cells can act as mediators for immune defense against tumors. Our previous study has demonstrated that persisting clonally expanded TRDV4 T cells might be relatively beneficial for the outcome of patients with T cell acute lymphoblastic leukemia after hematopoietic stem cell transplantation (HSCT). However, little is known about the distribution and clonality of the TRDV repertoire in T cell receptor (TCR) of γδ T cells and their effects on the clinical outcome of patients with acute myeloid leukemia (AML). The aim of this study was to assess whether the oligoclonal expansion of TCR Vδ T cells could be used as an immune biomarker for AML outcome.
  
  
  
  γδ T cells were sorted from the peripheral blood of 30 patients with untreated AML and 12 healthy donors. The complementarity-determining region 3 (CDR3) sizes of eight TCR Vδ subfamily genes (TRDV1 to TRDV8) were analyzed in sorted γδ T cells using RT-PCR and GeneScan. The most frequently expressed TRDV subfamilies in the AML patients were TRDV8 (86.67 %) and TRDV2 (83.33 %), and the frequencies for TRDV1, TRDV3, TRDV4, and TRDV6 were significantly lower than those in healthy individuals. The most frequent clonally expanded TRDV subfamilies in the AML patients included TRDV8 (56.67 %) and TRDV4 (40 %). The clonal expansion frequencies of the TRDV2 and TRDV4 T cells were significantly higher than those in healthy individuals, whereas a significantly lower TRDV1 clonal expansion frequency was observed in those with AML. Moreover, the oligoclones of TRDV4 and TRDV8 were independent protective factors for complete remission. Furthermore, the oligoclonal expansion frequencies of TRDV5 and TRDV6 in patients with relapse were significantly higher than those in non-recurrent cases.
  
  
  
  To the best of our knowledge, we characterized for the first time a significant alteration in the distribution and clonality of the TRDV subfamily members in γδ T cells sorted from AML patients. Clonally expanded TRDV4 and TRDV8 T cells might contribute to the immune response directed against AML, while oligoclonal TRDV5 and TRDV6 might occur in patients who undergo relapse. While the function of such γδ T cell clones requires further investigation, TRDV γδ T cell clones might be potential immune biomarkers for AML outcome.
authors.affiliation: Institute of Hematology, Medical College, Jinan University, Guangzhou, 510632, China.
authors.forename: Zhenyi
authors.initials: Z
authors.lastname: Jin
authors.affiliation: Institute of Hematology, Medical College, Jinan University, Guangzhou, 510632, China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Luo
authors.affiliation: Key Laboratory for Regenerative Medicine of Ministry of Education, Jinan University, Guangzhou, 510632, China.
authors.forename: Shuai
authors.initials: S
authors.lastname: Lu
authors.affiliation: Institute of Hematology, Medical College, Jinan University, Guangzhou, 510632, China.
authors.forename: Xinyu
authors.initials: X
authors.lastname: Wang
authors.affiliation: Institute of Hematology, Medical College, Jinan University, Guangzhou, 510632, China.
authors.forename: Zifan
authors.initials: Z
authors.lastname: He
authors.affiliation: Institute of Hematology, Medical College, Jinan University, Guangzhou, 510632, China.|Department of Hematology, First Affiliated Hospital, Jinan University, Guangzhou, 510632, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Lai
authors.affiliation: Institute of Hematology, Medical College, Jinan University, Guangzhou, 510632, China.
authors.forename: Shaohua
authors.initials: S
authors.lastname: Chen
authors.affiliation: Institute of Hematology, Medical College, Jinan University, Guangzhou, 510632, China.
authors.forename: Lijian
authors.initials: L
authors.lastname: Yang
authors.affiliation: Institute of Hematology, Medical College, Jinan University, Guangzhou, 510632, China. siulier@163.com.
authors.forename: Xiuli
authors.initials: X
authors.lastname: Wu
authors.affiliation: Institute of Hematology, Medical College, Jinan University, Guangzhou, 510632, China. yangqiuli@hotmail.com.|Department of Hematology, First Affiliated Hospital, Jinan University, Guangzhou, 510632, China. yangqiuli@hotmail.com.|Key Laboratory for Regenerative Medicine of Ministry of Education, Jinan University, Guangzhou, 510632, China. yangqiuli@hotmail.com.
authors.forename: Yangqiu
authors.initials: Y
authors.lastname: Li
mesh_terms: D015415:Biomarkers / Q000032:analysis; D001773:Blood Cells; D016022:Case-Control Studies; D049109:Cell Proliferation; D002999:Clone Cells; D022801:Complementarity Determining Regions / Q000235:genetics; D006801:Humans; D000075942:Intraepithelial Lymphocytes / Q000166:cytology*; D015470:Leukemia, Myeloid, Acute / Q000175:diagnosis* / Q000473:pathology; D016133:Polymerase Chain Reaction; D011379:Prognosis; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000032:analysis*
chemical_list: D015415:Biomarkers; D022801:Complementarity Determining Regions; D016692:Receptors, Antigen, T-Cell, gamma-delta
keywords: Acute myeloid leukemia; Clonality; T cell receptor; γδ T cells