title: Production of endothelial progenitor cells obtained from human Wharton's jelly using different culture conditions.
abstract: Endothelial progenitor cells (EPC) participate in revascularization and angiogenesis. EPC can be cultured in vitro from mononuclear cells of peripheral blood, umbilical cord blood or bone marrow; they also can be transdifferentiated from mesenchymal stem cells (MSC). We isolated EPCs from Wharton's jelly (WJ) using two methods. The first method was by obtaining MSC from WJ and characterizing them by flow cytometry and their adipogenic and osteogenic differentiation, then applying endothelial growth differentiating media. The second method was by direct culture of cells derived from WJ into endothelial differentiating media. EPCs were characterized by morphology, Dil-LDL uptake/UEA-1 immunostaining and testing the expression of endothelial markers by flow cytometry and RT-PCR. We found that MSC derived from WJ differentiated into endothelial-like cells using simple culture conditions with endothelium induction agents in the medium.
authors.affiliation: a Department of Clinical and Chemical Pathology , Cairo , Egypt.
authors.forename: S A
authors.initials: SA
authors.lastname: Zayed
authors.affiliation: a Department of Clinical and Chemical Pathology , Cairo , Egypt.
authors.forename: T M
authors.initials: TM
authors.lastname: Gaafar
authors.affiliation: a Department of Clinical and Chemical Pathology , Cairo , Egypt.
authors.forename: R M
authors.initials: RM
authors.lastname: Samy
authors.affiliation: b Department of Biochemistry , Cairo , Egypt.
authors.forename: D
authors.initials: D
authors.lastname: Sabry
authors.affiliation: c Department of Obstetrics and Gynecology , Cairo University , Cairo , Egypt.
authors.forename: A S
authors.initials: AS
authors.lastname: Nasr
authors.affiliation: a Department of Clinical and Chemical Pathology , Cairo , Egypt.
authors.forename: Fa Abdel
authors.initials: FA
authors.lastname: Maksoud
mesh_terms: D018929:Cell Culture Techniques; D002454:Cell Differentiation / Q000502:physiology*; D049109:Cell Proliferation / Q000502:physiology; D002478:Cells, Cultured; D066026:Endothelial Progenitor Cells / Q000166:cytology*; D005434:Flow Cytometry; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D010012:Osteogenesis / Q000502:physiology*; D059631:Wharton Jelly / Q000166:cytology*
keywords: Wharton's jelly; culture; endothelium; human tissue; markers; mesenchymal stem cells; progenitor cells