title: Towards understanding the distribution and tumor targeting of sericin regulated spherical calcium phosphate nanoparticles.
abstract: The study of calcium phosphate (CaP) nanoparticles in vivo is still incomplete, which has limited their applications for biomedical delivery. Herein, we synthesized amorphous spherical calcium phosphate (S-CaP) nanoparticles with an average size of 80 nm via a co-precipitation method in the presence of silk sericin as the regulation template. S-CaP was labeled by the near-infrared dye reagent DiR, and then, the labeled nanoparticles (S-CaP@DiR) were used to investigate the distribution and degradation in healthy mice by IVIS and TEM. The results showed that the S-CaP nanoparticles were mainly distributed in the liver, and ∼90% of them (500 μg) could be degraded by the liver within 2 weeks. Tumor-bearing mice were then prepared, and the S-CaP was injected intravenously. Strikingly, the nanoparticles can effectively target solid tumors in cancer cell-bearing mice, indicating that the solid tumor was a foundation for the enrichment of the nanoparticles by the EPR effect, which showed the important potential of biodegradable inorganic nanoparticles in clinical drugs for tumor therapy.
authors.affiliation: Institute of Biomaterials and Marine Biological Resources, College of Life Sciences, Zhejiang Sci-Tech University, Hangzhou, 310018, China.|Center for Biomaterials and Biopathways, Department of Chemistry, Zhejiang University, Hangzhou, Zhejiang, 310027, China.
authors.forename: Ruibo
authors.initials: R
authors.lastname: Zhao
authors.affiliation: Institute of Biomaterials and Marine Biological Resources, College of Life Sciences, Zhejiang Sci-Tech University, Hangzhou, 310018, China.
authors.forename: Xiaoyuan
authors.initials: X
authors.lastname: Ren
authors.affiliation: Institute of Biomaterials and Marine Biological Resources, College of Life Sciences, Zhejiang Sci-Tech University, Hangzhou, 310018, China.
authors.forename: Chungang
authors.initials: C
authors.lastname: Xie
authors.affiliation: Institute of Biomaterials and Marine Biological Resources, College of Life Sciences, Zhejiang Sci-Tech University, Hangzhou, 310018, China.|The Key Laboratory of Advanced Textile Materials and Manufacturing Technology of Ministry of Education, College of Materials and Textile, Zhejiang Sci-Tech University, Hangzhou, 310018, China.
authors.forename: Xiangdong
authors.initials: X
authors.lastname: Kong
mesh_terms: D000818:Animals; D002130:Calcium Phosphates / Q000008:administration & dosage / Q000138:chemical synthesis / Q000627:therapeutic use*; D045744:Cell Line, Tumor; D002470:Cell Survival; D006801:Humans; D051379:Mice; D008813:Mice, Inbred ICR; D008863:Microspheres; D053758:Nanoparticles / Q000008:administration & dosage / Q000737:chemistry* / Q000378:metabolism / Q000627:therapeutic use; D009369:Neoplasms / Q000188:drug therapy*; D010316:Particle Size; D047030:Sericins / Q000008:administration & dosage / Q000737:chemistry* / Q000627:therapeutic use; D014162:Transfection
chemical_list: D002130:Calcium Phosphates; D047030:Sericins; C519480:amorphous calcium phosphate
keywords: calcium phosphate; detection; in vivo; nanoparticles