title: Quantitative secretomic analysis of pancreatic cancer cells in serum-containing conditioned medium.
abstract: Pancreatic cancer is a highly metastatic and chemo-resistant disease. Secreted proteins involved in cell-cell interactions play an important role in changing the tumor microenvironment. Previous studies generally focus on the secretome of cancer cell line from serum-free media, due to the serious interference of fetal bovine serum (FBS). However, serum-starvation may alter expression patterns of secreted proteins. Hence, efforts to decrease the interference of serum in proteomic analysis of serum-containing media have been hampered to quantitatively measure the tumor secretion levels. Recently, the metabolic labeling, protein equalization, protein fractionation and filter-aided sample preparation (FASP) strategy (MLEFF) has been successfully used to avoid the disturbance of serum on secretome analysis. Here, this efficient method was applied for comparative secretome analysis of two hamster pancreatic cancer cells with differentially metastatic potentials, enabling the observation of 161 differentially expressed proteins, including 106 proteins that had been previously reported and detected in plasma. By integrated analysis of our data and publicly available bioinformatics resources, we found that a combination panel consisting of CDH3, PLAU, and LFNG might improve the prognosis of overall pancreatic cancer survival. These secreted proteins may serve as a potential therapeutic targets for pancreatic cancer metastasis.
authors.affiliation: 1st Department of general surgery, Shengjing Hospital, China Medical University, Shenyang 110004, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Liu
authors.affiliation: Key Lab of Separation Sciences for Analytical Chemistry, National Chromatographic R. &A. Center, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China.
authors.forename: Yejing
authors.initials: Y
authors.lastname: Weng
authors.affiliation: Key Lab of Separation Sciences for Analytical Chemistry, National Chromatographic R. &A. Center, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China.
authors.forename: Zhigang
authors.initials: Z
authors.lastname: Sui
authors.affiliation: 1st Department of general surgery, Shengjing Hospital, China Medical University, Shenyang 110004, China.
authors.forename: Yunhao
authors.initials: Y
authors.lastname: Wu
authors.affiliation: 1st Department of general surgery, Shengjing Hospital, China Medical University, Shenyang 110004, China.
authors.forename: Xiangli
authors.initials: X
authors.lastname: Meng
authors.affiliation: 1st Department of general surgery, Shengjing Hospital, China Medical University, Shenyang 110004, China.
authors.forename: Mengwei
authors.initials: M
authors.lastname: Wu
authors.affiliation: 1st Department of general surgery, Shengjing Hospital, China Medical University, Shenyang 110004, China.
authors.forename: Haoyi
authors.initials: H
authors.lastname: Jin
authors.affiliation: 1st Department of general surgery, Shengjing Hospital, China Medical University, Shenyang 110004, China.
authors.forename: Xiaodong
authors.initials: X
authors.lastname: Tan
authors.affiliation: Key Lab of Separation Sciences for Analytical Chemistry, National Chromatographic R. &A. Center, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China.
authors.forename: Lihua
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Key Lab of Separation Sciences for Analytical Chemistry, National Chromatographic R. &A. Center, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China.
authors.forename: Yukui
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D000818:Animals; D014408:Biomarkers, Tumor / Q000378:metabolism; D006224:Cricetinae; D017077:Culture Media, Conditioned / Q000378:metabolism*; D015972:Gene Expression Regulation, Neoplastic; D063990:Gene Ontology; D052138:Genes, Neoplasm; D006801:Humans; D009363:Neoplasm Proteins / Q000378:metabolism; D010190:Pancreatic Neoplasms / Q000235:genetics / Q000378:metabolism*; D011485:Protein Binding; D020543:Proteome / Q000378:metabolism; D040901:Proteomics / Q000379:methods*; D015203:Reproducibility of Results; D044967:Serum / Q000378:metabolism*; D015398:Signal Transduction; D016019:Survival Analysis; D014407:Tumor Cells, Cultured
chemical_list: D014408:Biomarkers, Tumor; D017077:Culture Media, Conditioned; D009363:Neoplasm Proteins; D020543:Proteome