title: Parkinson's disease-like motor and non-motor symptoms in rotenone-treated zebrafish.
abstract: The pesticide rotenone is widely used to produce Parkinson's disease (PD)-like symptoms in rodents, but few studies have examined whether rotenone-treated zebrafish can serve as an animal model of PD. Here, we report that 4 weeks of rotenone treatment induced motor and non-motor PD-like symptoms in adult zebrafish. Compared with control fish, rotenone-treated fish spent less time swimming at a fast speed, indicating a deficit in motor function. In the light-dark box test, rotenone-treated fish exhibited longer latencies to enter the dark compartment and spent more time in the light compartment, reflecting anxiety- and depression-like behavior. Furthermore, rotenone-treated fish showed less of an olfactory preference for amino acid, indicating olfactory dysfunction. These behavioral symptoms were associated with decreased levels of dopamine in the brains of rotenone-treated fish. Taken together, these results suggest that rotenone-treated zebrafish are a suitable model of PD.
authors.affiliation: Department of Neurology and Suzhou Clinical Research Center of Neurological Disease, The Second Affiliated Hospital of Soochow University, Suzhou 215004, China; Institutes of Neuroscience, Soochow University, Suzhou 215123, China.
authors.forename: Yali
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Institutes of Neuroscience, Soochow University, Suzhou 215123, China.
authors.forename: Wenwen
authors.initials: W
authors.lastname: Liu
authors.affiliation: Institutes of Neuroscience, Soochow University, Suzhou 215123, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Yang
authors.affiliation: Institutes of Neuroscience, Soochow University, Suzhou 215123, China.
authors.forename: Fen
authors.initials: F
authors.lastname: Wang
authors.affiliation: Institutes of Neuroscience, Soochow University, Suzhou 215123, China.
authors.forename: Yizhen
authors.initials: Y
authors.lastname: Sima
authors.affiliation: Center for Circadian Clock, Soochow University, Suzhou 215123, China; School of Biology & Basic Medical Sciences, Medical College, Soochow University, Suzhou 215123 Jiangsu, China.
authors.forename: Zhao-Min
authors.initials: ZM
authors.lastname: Zhong
authors.affiliation: Center for Circadian Clock, Soochow University, Suzhou 215123, China; School of Biology & Basic Medical Sciences, Medical College, Soochow University, Suzhou 215123 Jiangsu, China.
authors.forename: Han
authors.initials: H
authors.lastname: Wang
authors.affiliation: Institutes of Neuroscience, Soochow University, Suzhou 215123, China.
authors.forename: Li-Fang
authors.initials: LF
authors.lastname: Hu
authors.affiliation: Department of Neurology and Suzhou Clinical Research Center of Neurological Disease, The Second Affiliated Hospital of Soochow University, Suzhou 215004, China; Institutes of Neuroscience, Soochow University, Suzhou 215123, China. Electronic address: liuchunfeng@suda.edu.cn.
authors.forename: Chun-Feng
authors.initials: CF
authors.lastname: Liu
mesh_terms: D000818:Animals; D001007:Anxiety / Q000209:etiology*; D003623:Dark Adaptation / Q000187:drug effects; D004195:Disease Models, Animal; D005786:Gene Expression Regulation / Q000187:drug effects; D007306:Insecticides / Q000633:toxicity*; D008124:Locomotion / Q000187:drug effects; D008297:Male; D018377:Neurotransmitter Agents / Q000378:metabolism; D000857:Olfaction Disorders / Q000209:etiology*; D010302:Parkinson Disease, Secondary / Q000139:chemically induced* / Q000150:complications*; D012333:RNA, Messenger / Q000378:metabolism; D012402:Rotenone / Q000633:toxicity*; D015027:Zebrafish; D029961:Zebrafish Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D007306:Insecticides; D018377:Neurotransmitter Agents; D012333:RNA, Messenger; D029961:Zebrafish Proteins; D012402:Rotenone
keywords: Non-motor symptoms; Parkinson’s disease; Rotenone; Zebrafish