title: Altered extracellular ATP, ADP, and AMP hydrolysis in blood serum of sedentary individuals after an acute, aerobic, moderate exercise session.
abstract: Nucleotidases participate in the regulation of physiological and pathological events, such as inflammation and coagulation. Exercise promotes distinct adaptations, and can influence purinergic signaling. In the present study, we investigated soluble nucleotidase activities in the blood serum of sedentary young male adults at pre- and post-acute moderate aerobic exercise. In addition, we evaluated how this kind of exercise could influence adenine nucleotide concentrations in the blood serum. Sedentary individuals were submitted to moderate aerobic exercise on a treadmill; blood samples were collected pre- and post-exercise, and serum was separated for analysis. Results showed increases in ATP, ADP, and AMP hydrolysis post-exercise, compared to pre-exercise values. The ecto-nucleotide pyrophosphatase/phosphodiesterase was also evaluated, showing an increased activity post-exercise, compared to pre-exercise. Purine levels were analyzed by HPLC in the blood serum, pre- and post-exercise. Decreased levels of ATP and ADP were found post-exercise, in contrast with pre-exercise values. Conversely, post-exercise levels of adenosine and inosine increased compared to pre-exercise levels. Our results indicate an influence of acute exercise on ATP metabolism, modifying enzymatic behavior to promote a protective biological environment.
authors.affiliation: Programa de Pós-Graduação em Medicina: Ciências Médicas, Faculdade de Medicina, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil.
authors.forename: Cesar Eduardo Jacintho
authors.initials: CE
authors.lastname: Moritz
authors.affiliation: Programa de Pós-Graduação em Ciência do Movimento Humano, Escola de Educação Física, Universidade Federado do Rio Grande do Sul, Porto Alegre, RS, Brazil.|Departamento de Educação Física, Universidade Regional Integrada do Alto Uruguai e das Missões, São Luiz Gonzaga, RS, Brazil.
authors.forename: Bruno Costa
authors.initials: BC
authors.lastname: Teixeira
authors.affiliation: Progama de Pós-Graduação em Ciências Biológicas: Bioquímica, Instituto de Ciências Básicas, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil.
authors.forename: Liliana
authors.initials: L
authors.lastname: Rockenbach
authors.affiliation: Programa de Pós-Graduação em Ciência do Movimento Humano, Escola de Educação Física, Universidade Federado do Rio Grande do Sul, Porto Alegre, RS, Brazil.
authors.forename: Alvaro
authors.initials: A
authors.lastname: Reischak-Oliveira
authors.affiliation: Departamento de Bioquímica, Instituto de Ciências Básicas, Universidade Federal do Rio Grande do Sul, Rua Ramiro Barcelos, 2600 - Anexo, Porto Alegre, RS, 90035-003, Brazil.|Departamento de Ciências Morfológicas, Instituto de Ciências Básicas, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil.
authors.forename: Emerson André
authors.initials: EA
authors.lastname: Casali
authors.affiliation: Programa de Pós-Graduação em Medicina: Ciências Médicas, Faculdade de Medicina, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil. abattastini@gmail.com.|Progama de Pós-Graduação em Ciências Biológicas: Bioquímica, Instituto de Ciências Básicas, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil. abattastini@gmail.com.|Departamento de Bioquímica, Instituto de Ciências Básicas, Universidade Federal do Rio Grande do Sul, Rua Ramiro Barcelos, 2600 - Anexo, Porto Alegre, RS, 90035-003, Brazil. abattastini@gmail.com.
authors.forename: Ana Maria Oliveira
authors.identifier: http://orcid.org/0000-0001-6068-3278
authors.initials: AM
authors.lastname: Battastini
mesh_terms: D000244:Adenosine Diphosphate / Q000097:blood*; D000249:Adenosine Monophosphate / Q000097:blood*; D000255:Adenosine Triphosphate / Q000097:blood*; D000328:Adult; D015444:Exercise; D006801:Humans; D006868:Hydrolysis; D008297:Male
chemical_list: D000249:Adenosine Monophosphate; D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate
keywords: Exercise; Nucleotidases; Purinergic signaling; Sedentary