title: Assaying Germination and Seedling Responses of Arabidopsis to Karrikins.
abstract: Karrikins are a small family of naturally occurring plant growth regulators present in the smoke and char produced from burning plant material in wildfires. They can stimulate germination of dormant seed and can influence seedling morphogenesis. Although Arabidopsis thaliana is not considered to be a smoke-responsive species, karrikins will stimulate seed germination under the appropriate circumstances and will cause repression of hypocotyl elongation in low light. This chapter describes how to conduct assays of the activity of karrikins on Arabidopsis seeds and seedlings. The methods presented can potentially be modified for use in a range of Arabidopsis genotypes or in other plant species.
authors.affiliation: Australian Research Council Centre of Excellence in Plant Energy Biology, University of Western Australia, Crawley, WA, 6009, Australia.|School of Chemistry and Biochemistry, University of Western Australia, Crawley, WA, 6009, Australia.
authors.forename: Mark T
authors.initials: MT
authors.lastname: Waters
authors.affiliation: School of Chemistry and Biochemistry, University of Western Australia, Crawley, WA, 6009, Australia.
authors.forename: Gavin R
authors.initials: GR
authors.lastname: Flematti
authors.affiliation: School of Biological Sciences, University of Tasmania, Hobart, TAS, 7001, Australia. Steven.smith@utas.edu.au.|State Key Laboratory of Plant Genomics & National Center for Plant Gene Research (Beijing), Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, 100101, China. Steven.smith@utas.edu.au.
authors.forename: Steven M
authors.initials: SM
authors.lastname: Smith
mesh_terms: D017360:Arabidopsis / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000378:metabolism*; D005663:Furans / Q000378:metabolism*; D018506:Gene Expression Regulation, Plant / Q000502:physiology; D005838:Genotype; D018525:Germination / Q000502:physiology*; D018546:Hypocotyl / Q000378:metabolism; D008027:Light; D057445:Plant Dormancy / Q000502:physiology; D010937:Plant Growth Regulators / Q000378:metabolism*; D011714:Pyrans / Q000378:metabolism*; D036226:Seedlings / Q000378:metabolism*; D012639:Seeds / Q000378:metabolism; D012906:Smoke
chemical_list: D029681:Arabidopsis Proteins; D005663:Furans; D010937:Plant Growth Regulators; D011714:Pyrans; D012906:Smoke
keywords: Arabidopsis thaliana; Dormancy; Hypocotyl elongation; Karrikins; Photomorphogenesis; Seed germination