title: Effect of Methanolic Leaf Extract ofTalinum triangulare(Jacq). Willd. on Biochemical Parameters in Diet induced Dyslipidemia Wistar Rats.
abstract: OBJECTIVE
  To investigate the effect of methanolic leaf extract ofTalinum triangulareon hematological parameters, enzymatic and non-enzymatic antioxidant status, and serum lipid in Wistar rats fed standard laboratory, or 2% cholesterol-enrich diet.
  
  
  METHODS
  Wistar rats (180-210g) divided into six groups of six animals (males) each were fed 2% cholesterol-enriched diet and orally treated with 0.9% saline or extract ofTalinum triangulare(250, 500, and 1000 mg/kg per body weight) daily for eight weeks. Lipid profile, lipid peroxidation (MDA), hematological parameters, and their functional indices and serum antioxidant enzymes (catalase, glutathione -S-transferase, and superoxide dismutase) activities and glutathione status were assessed in normal and diet-induced hypercholesterolemic extract treated rats and compared with the rats treated with 100 mg/kg per bwt standard drug gemfibrozil.
  
  
  RESULTS
  A significant (P< 0.05) increase in lipid profile (total glyceride, total cholestrol, low-density lipoprotein, and very low-density lipoprotein), MDA and reduction (P< 0.05) in enzymatic and nonenzymatic antioxidant status coupled with alterations in hematological parameters was observed in the serum of hypercholesterolemic rats when compared with animals on a normal diet. Coadministration of methanolic leaf extracts ofTalinum triangulareor gemfibrozil significantly (P< 0.05) restored the elevated serum lipid profile, MDA, and the deranged hematological parameters to near normal. The extract also protected against hypercholesterolemic-induced diminished enzymatic and nonenzymatic antioxidant status. The activities of the plant extract are dose (250, 500, and 1000 mg/kg) dependent and it compared favorably with the standard drug gemfibrozil.
  
  
  CONCLUSIONS
  The present study suggested that the extract ofTalinum triangularemight protect against hypercholesterolemic-induced altered lipid profiles, oxidative stress, and also improve the status of antioxidant defense system and hematopoiesis.
  
  
  CONCLUSIONS
  Elevated lipid profile (total glyceride, total cholestrol, low-density lipoprotein, and very low-density lipoprotein), lipid peroxidation (MDA), and reduced enzymatic and nonenzymatic antioxidant status coupled with alterations in hematological parameters was observed in the serum of hypercholesterolemic rats when compared with animals on a normal dietCoadministration of methanolic leaf extracts ofTalinum triangularesignificantly (P< 0.05) restored the elevated serum lipid profile, MDA, and the deranged hematological parameters to near normal.The extract also protected against hypercholesterolemic-induced diminished enzymatic and bnonenzymatic antioxidant status.The activities of the plant extract was dose-dependent and it compared favorably with the standard drug gemfibrozil.Abbreviations used:Lipid peroxidation (MDA), (catalase (CAT), glutathione-S-transferase (GST), superoxide dismutase (SOD), glutathione (GSH), Thrombocytes indices (PLT), Red blood cell (RBC), Packed cell volume (PVC), Mean corpuscular hemoglobin(MCH), Mean corpuscular hemoglobin concentration (MCHC), Total glyceride (TG), Very low density lipoprotein (VLDL), Total cholesterol (TC), Low density lipoprotein (LDL), High density lipoprotein (HDL) and 3-Hydroxy-3-methyl-glutaryl-CoA reductase(HMG-CoA).
authors.affiliation: Department of Biochemistry, Ladoke Akintola University of Technology, Ogbomoso, Oyo State, Nigeria; Medicinal Plant and Economic Development (MPED) Research Center. University of Fort Hare, Alice, South Africa.
authors.forename: Olubukola Sinbad
authors.initials: OS
authors.lastname: Olorunnisola
authors.affiliation: Department of Biochemistry, Ladoke Akintola University of Technology, Ogbomoso, Oyo State, Nigeria.
authors.forename: Adewale
authors.initials: A
authors.lastname: Adetutu
authors.affiliation: Medicinal Plant and Economic Development (MPED) Research Center. University of Fort Hare, Alice, South Africa.
authors.forename: Anthony Jide
authors.initials: AJ
authors.lastname: Afolayan
authors.affiliation: Department of Biochemistry, Ladoke Akintola University of Technology, Ogbomoso, Oyo State, Nigeria.
authors.forename: Abiodun Olusoji
authors.initials: AO
authors.lastname: Owoade
keywords: Talinum triangulare; anti-lipidemia; antioxidant; antioxidant enzymes; hematopoiesis