title: Interaction of a 22 kDa Peptidyl Prolylcis/transIsomerase with the Heat Shock Protein DnaK inVibrio anguillarum.
abstract: Peptidyl prolylcis/transisomerases (PPIases) catalyze thecis/transisomerization of peptidyl-prolyl peptide bonds preceding prolines. We investigated the protein-protein interaction between a 22 kDa PPIase (VaFKBP22, an FK506-binding protein) and the molecular chaperone DnaK derived from Vibrio anguillarum O1 (VaDnaK) using GST pull-down assays and a bacterial two-hybrid system for in vivo and in vitro studies, respectively. Furthermore, we analyzed the three-dimensional structure of the protein-protein interaction. Based on our results, VaFKBP22 appears to act as a cochaperone of VaDnaK, and contributes to protein folding and stabilization via its peptidyl-prolylcis/transisomerization activity.
authors.affiliation: Department of Biotechnology, Pukyong National University, Busan 48513, Republic of Korea.
authors.forename: Dong Seop
authors.initials: DS
authors.lastname: Kang
authors.affiliation: Department of Biotechnology, Pukyong National University, Busan 48513, Republic of Korea.
authors.forename: Soo Young
authors.initials: SY
authors.lastname: Moon
authors.affiliation: Department of Biotechnology, Pukyong National University, Busan 48513, Republic of Korea.
authors.forename: Hwa Jin
authors.initials: HJ
authors.lastname: Cho
authors.affiliation: Department of Biotechnology, Pukyong National University, Busan 48513, Republic of Korea.
authors.forename: Jong Min
authors.initials: JM
authors.lastname: Lee
authors.affiliation: Department of Biotechnology, Pukyong National University, Busan 48513, Republic of Korea.
authors.forename: In-Soo
authors.initials: IS
authors.lastname: Kong
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry / Q000378:metabolism*; D001665:Binding Sites; D006360:Heat-Shock Proteins / Q000737:chemistry / Q000378:metabolism*; D008958:Models, Molecular; D008970:Molecular Weight; D019696:Peptidylprolyl Isomerase / Q000737:chemistry / Q000378:metabolism*; D011485:Protein Binding; D011487:Protein Conformation; D054730:Protein Interaction Domains and Motifs; D025941:Protein Interaction Mapping; D055503:Protein Multimerization; D014733:Vibrio / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D006360:Heat-Shock Proteins; D019696:Peptidylprolyl Isomerase
keywords: DnaK; Peptidyl prolyl cis/trans isomerase (PPIase); Vibrio anguillarum; protein-protein interaction