title: Molecular characterization ofMyxobolus cuttacki(Myxozoa, Myxosporea, Bivalvulida) infecting gill lamellae of minor carpLabeo bata(Ham.).
abstract: As new pathogenic strains are emerging and threatening aquaculture development, myxosporeans (Myxozoa) are receiving much attention in recent years. Myxosporean taxonomy is traditionally based on morphology of the myxospore stage. Molecular data on Indian myxosporeans are rare. In this report, the 18S rRNA gene sequence of Myxobolus cuttacki infecting gill lamellae of minor carpLabeo bata(Ham.) and its phylogenetic relationship with other myxobolids are described for the first time. The plasmodia ofM. cuttackiwere 0.5-0.9 mm in size and whitish with a round to oval shape. The mean mature spore size was 16.10×7.05 μm. The 18S rRNA nucleotide sequence with 1703 bp ofM. cuttacki(accession number KF465682) clustered phylogenetically with otherMyxobolusspp. infecting cyprinid gills with 78-90% homogeneity. The gill lamellae infectingM. catmrigalae(KC933944) andM. orissae(KF448527) of Indian major carpCirrhinus mrigalafrom India, exhibited 86% and 81% homogeneity withM. cuttacki, respectively. The infection rate was low to moderate on the gills which can have a negative impact on respiratory and physiological functions and subsequently on fish production.
authors.affiliation: Department of Aquatic Animal Health, Faculty of Fishery Sciences, West Bengal University of Animal and Fishery Sciences, Kolkata 700094, India.
authors.forename: Sandya Chinna
authors.initials: SC
authors.lastname: Rajesh
authors.affiliation: Department of Aquatic Animal Health, Faculty of Fishery Sciences, West Bengal University of Animal and Fishery Sciences, Kolkata 700094, India.
authors.forename: Sayani
authors.initials: S
authors.lastname: Banerjee
authors.affiliation: Department of Aquatic Animal Health, Faculty of Fishery Sciences, West Bengal University of Animal and Fishery Sciences, Kolkata 700094, India.
authors.forename: Avijit
authors.initials: A
authors.lastname: Patra
authors.affiliation: Department of Aquatic Animal Health, Faculty of Fishery Sciences, West Bengal University of Animal and Fishery Sciences, Kolkata 700094, India.
authors.forename: Gadadhar
authors.initials: G
authors.lastname: Dash
authors.affiliation: Department of Aquatic Animal Health, Faculty of Fishery Sciences, West Bengal University of Animal and Fishery Sciences, Kolkata 700094, India.
authors.forename: Thangapalam Jawahar
authors.initials: TJ
authors.lastname: Abraham
keywords: 18S rRNA; Labeo bata; Myxobolus cuttacki; Phylogenetic relationship