title: Aspirin upregulates αB-Crystallin to protect the myocardium against heat stress in broiler chickens.
abstract: We established in vivo and in vitro models to investigate the role of αB-Crystallin (CryAB) and assess the ability of aspirin (ASA) to protect the myocardium during prolonged heat stress. Thirty-day-old chickens were divided into three groups (n = 90): heat stress (HS, 40±1 °C); ASA(-)HS(+), 1 mg/kg ASA orally 2 h before heat stress; and ASA(+)HS(-), pretreated with aspirin, no heat stress (25 °C). Hearts were excised after 0, 1, 2, 3, 5, 7, 10, 15 and 24 h. Heat stress increased body temperature, though the ASA(-)HS(+) group had significantly higher temperatures than the ASA(+)HS(+) group at all time points. Compared to ASA(+)HS(+), the ASA(-)HS(+) group displayed increased sensitivity to heat stress. Pathological analysis revealed the ASA (+)HS(+) myocardium showed less severe changes (narrowed, chaotic fibers; fewer necrotic cells) than the ASA(-)HS(+) group (bleeding and extensive cell death). In vitro, ASA-pretreatment significantly increased primary chicken myocardial cell survival during heat stress. ELISAs indicated ASA induced CryAB in vivo to protect against heat stress-induced myocardial damage, but ASA did not induce CryAB in primary chicken myocardial cells. The mechanisms by which ASA induces the expression of CryAB in vivo and protects the myocardium during heat stress merit further research.
authors.affiliation: College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China.
authors.forename: Shu
authors.initials: S
authors.lastname: Tang
authors.affiliation: College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Yin
authors.affiliation: College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China.
authors.forename: Erbao
authors.initials: E
authors.lastname: Song
authors.affiliation: College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China.
authors.forename: Hongbo
authors.initials: H
authors.lastname: Chen
authors.affiliation: College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China.
authors.forename: Yanfen
authors.initials: Y
authors.lastname: Cheng
authors.affiliation: College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China.
authors.forename: Xiaohui
authors.initials: X
authors.lastname: Zhang
authors.affiliation: College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China.
authors.forename: Endong
authors.initials: E
authors.lastname: Bao
authors.affiliation: Institute for Animal Hygiene, Animal Welfare and Farm Animal Behaviour, University of Veterinary Medicine Hannover, Foundation, Hannover, 30173, Germany.
authors.forename: Joerg
authors.initials: J
authors.lastname: Hartung
mesh_terms: D000818:Animals; D001241:Aspirin / Q000494:pharmacology*; D030161:Avian Proteins / Q000096:biosynthesis*; D002645:Chickens / Q000378:metabolism*; D018882:Heat Stress Disorders / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control* / Q000662:veterinary; D009206:Myocardium / Q000378:metabolism* / Q000473:pathology; D011201:Poultry Diseases / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control*; D038203:alpha-Crystallin B Chain / Q000096:biosynthesis*
chemical_list: D030161:Avian Proteins; D038203:alpha-Crystallin B Chain; D001241:Aspirin