title: P2rx4 deficiency in mice alleviates allergen-induced airway inflammation.
abstract: Compelling evidences point out a crucial role for extracellular nucleotides such as adenosine triphosphate (ATP) during inflammatory conditions. Once released into the extracellular space, ATP modulates migration, maturation and function of various inflammatory cells via activating of purinergic receptors of the P2Y- and P2X- family. P2RX4 is an ATP-guided ion channel expressed on structural cells such as alveolar epithelial and smooth muscle cells as well as inflammatory cells including macrophages, dendritic cells (DCs) and T cells. P2RX4 has been shown to interact with P2RX7 and promote NLRP3 inflammasome activation. Although P2RX7 has already been implicated in allergic asthma, the role of P2RX4 in airway inflammation has not been elucidated yet. Therefore, we used a selective pharmacological antagonist and genetic ablation to investigate the role of P2RX4 in an ovalbumin (OVA) driven model of allergen-induced airway inflammation (AAI). Both, P2RX4 antagonist 5-BDBD treatment and P2rx4 deficiency resulted in an alleviated broncho alveolar lavage fluid eosinophilia, peribronchial inflammation, Th2 cytokine production and bronchial hyperresponsiveness. Furthermore, P2rx4-deficient bone marrow derived DCs (BMDCs) showed a reduced IL-1ß production in response to ATP accompanied by a decreased P2rx7 expression and attenuated Th2 priming capacity compared to wild type (WT) BMDCs in vitro. Moreover, mice adoptively transferred with P2rx4-deficient BMDCs exhibit a diminished AAI in vivo. In conclusion our data suggests that P2RX4-signaling contributes to AAI pathogenesis by regulating DC mediated Th2 cell priming via modulating IL-1ß secretion and selective P2RX4-antagonists might be a new therapeutic option for allergic asthma.
authors.affiliation: Department of Pneumology, University Medical Centre Freiburg, Germany.
authors.forename: Andreas
authors.initials: A
authors.lastname: Zech
authors.affiliation: Department of Pneumology, University Medical Centre Freiburg, Germany.
authors.forename: Benjamin
authors.initials: B
authors.lastname: Wiesler
authors.affiliation: Department of Pneumology, University Medical Centre Freiburg, Germany.
authors.forename: Cemil Korcan
authors.initials: CK
authors.lastname: Ayata
authors.affiliation: Department of Pneumology, University Medical Centre Freiburg, Germany.
authors.forename: Tilmann
authors.initials: T
authors.lastname: Schlaich
authors.affiliation: Department of Pneumology, University Medical Centre Freiburg, Germany.
authors.forename: Thorsten
authors.initials: T
authors.lastname: Dürk
authors.affiliation: Department of Pneumology, University Medical Centre Freiburg, Germany.
authors.forename: Madelon
authors.initials: M
authors.lastname: Hoßfeld
authors.affiliation: Department of Pneumology, University Medical Centre Freiburg, Germany.
authors.forename: Nicolas
authors.initials: N
authors.lastname: Ehrat
authors.affiliation: Department of Pneumology, University Medical Centre Freiburg, Germany.
authors.forename: Sanja
authors.initials: S
authors.lastname: Cicko
authors.affiliation: Department of Pneumology, University Medical Centre Freiburg, Germany.
authors.forename: Marco
authors.initials: M
authors.lastname: Idzko
mesh_terms: D000255:Adenosine Triphosphate / Q000494:pharmacology; D019264:Adoptive Transfer; D000485:Allergens; D000818:Animals; D001570:Benzodiazepinones / Q000494:pharmacology; D001854:Bone Marrow Cells / Q000276:immunology / Q000378:metabolism; D016026:Bone Marrow Transplantation; D016535:Bronchial Hyperreactivity / Q000235:genetics / Q000276:immunology / Q000378:metabolism / Q000517:prevention & control*; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry; D016084:Bronchoconstriction; D002478:Cells, Cultured; D018920:Coculture Techniques; D016207:Cytokines / Q000378:metabolism; D003713:Dendritic Cells / Q000276:immunology / Q000378:metabolism; D004195:Disease Models, Animal; D020022:Genetic Predisposition to Disease; D006801:Humans; D008168:Lung / Q000187:drug effects / Q000276:immunology / Q000378:metabolism* / Q000503:physiopathology; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D010047:Ovalbumin; D010641:Phenotype; D011014:Pneumonia / Q000235:genetics / Q000276:immunology / Q000378:metabolism / Q000517:prevention & control*; D058911:Purinergic P2X Receptor Agonists / Q000494:pharmacology; D058920:Purinergic P2X Receptor Antagonists / Q000494:pharmacology; D039981:Pyroglyphidae / Q000276:immunology; D058848:Receptors, Purinergic P2X4 / Q000172:deficiency* / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D018418:Th2 Cells / Q000187:drug effects / Q000276:immunology / Q000378:metabolism
chemical_list: C587048:5-(3-bromophenyl)-1,3-dihydro-2H-benzofuro(3,2-e)-1,4-diazepin-2-one; D000485:Allergens; D001570:Benzodiazepinones; D016207:Cytokines; C548226:P2RX4 protein, human; C548227:P2rx4 protein, mouse; D058911:Purinergic P2X Receptor Agonists; D058920:Purinergic P2X Receptor Antagonists; D058848:Receptors, Purinergic P2X4; D000255:Adenosine Triphosphate; D010047:Ovalbumin
keywords: ATP; IL-1ß; Immune response; Immunity; Immunology and Microbiology Section; P2RX4; asthma; dendritic cells