title: Linolenic acid prevents early and advanced glycation end-products (AGEs) modification of albumin.
abstract: In this study, we report the protective effects of linolenic acid towards the formation of early (HbA1c) and advanced glycation end-products (AGEs) based on fluorescence, circular dichroism, confocal microscopy and molecular interaction studies. Linolenic acid was found to be a potent inhibitor of AGEs formed by both glucose and fructose. The HbA1c (early glycation product) level was found to be reduced to 7.4% when compared to glycated control (8.4%). Similarly, linolenic acid also inhibited the methylglyoxal mediated AGEs formation. Circular dichroism spectroscopy studies suggested that the protective effect of linolenic acid for the helical structure of albumin. The molecular interaction studies showed that linolenic acid interacts with arginine residues of albumin with high affinity. Results suggested linolenic acid to be a potent antiglycation compound and also it could be a better lead compound for AGE inhibition.
authors.affiliation: Molecular Biophysics Laboratory, School of Chemical and Biotechnology, SASTRA University, Thanjavur, 613401, India.
authors.forename: Govindarajan
authors.initials: G
authors.lastname: Prasanna
authors.affiliation: Molecular Biophysics Laboratory, School of Chemical and Biotechnology, SASTRA University, Thanjavur, 613401, India. Electronic address: saras@scbt.sastra.edu.
authors.forename: N T
authors.initials: NT
authors.lastname: Saraswathi
mesh_terms: D000818:Animals; D002417:Cattle; D017127:Glycation End Products, Advanced / Q000378:metabolism*; D006031:Glycosylation / Q000187:drug effects; D062105:Molecular Docking Simulation; D066329:Protein Aggregates / Q000187:drug effects; D011485:Protein Binding / Q000187:drug effects; D011487:Protein Conformation; D055550:Protein Stability / Q000187:drug effects; D012710:Serum Albumin, Bovine / Q000737:chemistry / Q000378:metabolism*; D017962:alpha-Linolenic Acid / Q000378:metabolism / Q000494:pharmacology*
chemical_list: D017127:Glycation End Products, Advanced; D066329:Protein Aggregates; D017962:alpha-Linolenic Acid; D012710:Serum Albumin, Bovine
keywords: Advanced glycation end-products; Confocal microscopy; Docking; HbA(1)c; Linolenic acid