title: Organ- and Growing Stage-Specific Expression of Solanesol Biosynthesis Genes in Nicotiana tabacum Reveals Their Association with Solanesol Content.
abstract: Solanesol is a noncyclic terpene alcohol that is composed of nine isoprene units and mainly accumulates in solanaceous plants, especially tobacco (Nicotiana tabacumL.). In the present study, RNA-seq analyses of tobacco leaves, stems, and roots were used to identify putative solanesol biosynthesis genes. Six 1-deoxy-d-xylulose 5-phosphate synthase (DXS), two 1-deoxy-d-xylulose 5-phosphate reductoisomerase (DXR), two 2-C-methyl-d-erythritol 4-phosphate cytidylyltransferase (IspD), four 4-diphosphocytidyl-2-C-methyl-d-erythritol kinase (IspE), two 2-C-methyl-d-erythritol 2,4-cyclo-diphosphate synthase (IspF), four 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase (IspG), two 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate reductase (IspH), six isopentenyl diphosphate isomerase (IPI), and two solanesyl diphosphate synthase (SPS) candidate genes were identified in the solanesol biosynthetic pathway. Furthermore, the twoN. tabacumSPS proteins (NtSPS1andNtSPS2), which possessed two conserved aspartate-rich DDxxD domains, were highly homologous with SPS enzymes from other solanaceous plant species. In addition, the solanesol contents of three organs and of leaves from four growing stages of tobacco plants corresponded with the distribution of chlorophyll. Our findings provide a comprehensive evaluation of the correlation between the expression of different biosynthesis genes and the accumulation of solanesol, thus providing valuable insight into the regulation of solanesol biosynthesis in tobacco.
authors.affiliation: Tobacco Research Institute of Chinese Academy of Agricultural Sciences, Qingdao 266101, China. yanning@caas.cn.
authors.forename: Ning
authors.initials: N
authors.lastname: Yan
authors.affiliation: Tobacco Research Institute of Chinese Academy of Agricultural Sciences, Qingdao 266101, China. zhanghongbo@caas.cn.
authors.forename: Hongbo
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Tobacco Research Institute of Chinese Academy of Agricultural Sciences, Qingdao 266101, China. zhangzhongfeng@caas.cn.
authors.forename: Zhongfeng
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: Guelph Food Research Center, Agriculture and Agri-Food Canada, Guelph, ON N1G 5C9, Canada. john.shi@agr.gc.ca.
authors.forename: John
authors.initials: J
authors.lastname: Shi
authors.affiliation: Department of Biology, University of Virginia, Charlottesville, VA 22904, USA. mpt9g@virginia.edu.
authors.forename: Michael P
authors.initials: MP
authors.lastname: Timko
authors.affiliation: Tobacco Research Institute of Chinese Academy of Agricultural Sciences, Qingdao 266101, China. duyongmei@caas.cn.
authors.forename: Yongmei
authors.initials: Y
authors.lastname: Du
authors.affiliation: Tobacco Research Institute of Chinese Academy of Agricultural Sciences, Qingdao 266101, China. liuxinmin@caas.cn.
authors.forename: Xinmin
authors.initials: X
authors.lastname: Liu
authors.affiliation: Tobacco Research Institute of Chinese Academy of Agricultural Sciences, Qingdao 266101, China. 13646810352@163.com.
authors.forename: Yanhua
authors.initials: Y
authors.lastname: Liu
mesh_terms: D000595:Amino Acid Sequence; D053898:Biosynthetic Pathways / Q000235:genetics*; D002734:Chlorophyll / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D009928:Organ Specificity / Q000235:genetics; D010802:Phylogeny; D018515:Plant Leaves / Q000378:metabolism; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D013729:Terpenes / Q000378:metabolism*; D014026:Nicotiana / Q000145:classification / Q000235:genetics* / Q000378:metabolism*
chemical_list: D010940:Plant Proteins; D013729:Terpenes; D002734:Chlorophyll; C017719:solanesol
keywords: Nicotiana tabacum; RNA-seq; chlorophyll; gene expression; solanesol; solanesyl diphosphate synthase