title: [Effect of CPNE7 siRNA on cell proliferation and osteogenic differentiation in human periodontal ligament cells].
abstract: OBJECTIVE
  To investigate the effect and mechanism of CPNE7 siRNA on proliferation and osteogenic differentiation in human periodontal ligament cells (hPDLs).
  
  
  METHODS
  hPDLs were isolated by enzyme digestion, transfected with pSUPER-CPNE7 in order to knock down CPNE7. The expression of CPNE7 mRNA and protein was detected by Western blot and RT-PCR; ELISA was carried out for the activity of NF-κB; hPDLs were pretreated with PDTC (10 μmol/L) for 30 min, CCK8 was used to evaluate the proliferation; ALP activity was assayed with ELISA; The expression of RUNX2, OSX and OCN was measured with RT-PCR and Western blot. The data were analyzed with SPSS11.0 software package.
  
  
  RESULTS
  After transfection with pSUPER-CPNE7, CPNE7 expression was significantly decreased (P<0.05). ELISA assay indicated that CPNE7 siRNA enhanced NF-κB activity. CCK8 and RT-PCR assay showed that CPNE7 siRNA inhibited cell proliferation, ALP activity and decreased the expression of RUNX2, OSX and OCN in hPDLs; however, these effects were abolished by PDTC.
  
  
  CONCLUSIONS
  CPNE7 siRNA inhibits the proliferation and osteogenic differentiation of hPDLs through NF-κB pathway.
authors.affiliation: Department of Prosthodontics, Affiliated Hospital of Yan'an University. Yan'an 716000, Shaanxi Province, China. E-mail:1149577790@qq.com.
authors.forename: Cai-Hong
authors.initials: CH
authors.lastname: Liu
authors.forename: Hai-Yan
authors.initials: HY
authors.lastname: Hu
mesh_terms: D000469:Alkaline Phosphatase; D015153:Blotting, Western; D002454:Cell Differentiation; D049109:Cell Proliferation; D006801:Humans; D008565:Membrane Proteins; D016328:NF-kappa B; D010012:Osteogenesis; D010513:Periodontal Ligament; D034741:RNA, Small Interfering; D015398:Signal Transduction; D014162:Transfection
chemical_list: C400933:CPNE7 protein, human; D008565:Membrane Proteins; D016328:NF-kappa B; D034741:RNA, Small Interfering; D000469:Alkaline Phosphatase