title: Continuous cultivation of lipid rich microalga Chlorella sp. FC2 IITG for improved biodiesel productivity via control variable optimization and substrate driven pH control.
abstract: A novel two-stage continuous heterotrophic cultivation of Chlorella sp. FC2 IITG was demonstrated for enhanced lipid productivity. Initially, effect of control variable e.g. dilution rate and feed stream substrate concentrations on biomass productivity was evaluated. This showed significant variation in biomass productivity from 2.4gL-1day-1to 11.2gL-1day-1. Further, these control variables were optimized by using multi-nutrient mechanistic model for maximizing the biomass productivity. Finally, continuous production of lipid rich algal biomass was demonstrated in two sequential bioreactors for enhanced lipid productivity. The biomass productivity of 92.7gL-1day-1was observed in the first reactor which was operated at model predicted optimal substrate concentrations of feed stream. The intracellular neutral lipid enrichment by acetate addition resulted in lipid productivity of 9.76gL-1day-1in the second reactor. Both the biomass and lipid productivities obtained from current study are significantly high amongst similarly reported literatures.
authors.affiliation: Department of Biosciences and Bioengineering, Indian Institute of Technology, Guwahati, Assam 781039, India.
authors.forename: Basavaraj
authors.initials: B
authors.lastname: Palabhanvi
authors.affiliation: Department of Biosciences and Bioengineering, Indian Institute of Technology, Guwahati, Assam 781039, India.
authors.forename: Muthusivaramapandian
authors.initials: M
authors.lastname: Muthuraj
authors.affiliation: Centre for Energy, Indian Institute of Technology, Guwahati, Assam 781039, India.
authors.forename: Vikram
authors.initials: V
authors.lastname: Kumar
authors.affiliation: Department of Biosciences and Bioengineering, Indian Institute of Technology, Guwahati, Assam 781039, India.
authors.forename: Mayurketan
authors.initials: M
authors.lastname: Mukherjee
authors.affiliation: Department of Biosciences and Bioengineering, Indian Institute of Technology, Guwahati, Assam 781039, India.
authors.forename: Saumya
authors.initials: S
authors.lastname: Ahlawat
authors.affiliation: Department of Biosciences and Bioengineering, Indian Institute of Technology, Guwahati, Assam 781039, India; Centre for Energy, Indian Institute of Technology, Guwahati, Assam 781039, India. Electronic address: debasishd@iitg.ernet.in.
authors.forename: Debasish
authors.initials: D
authors.lastname: Das
mesh_terms: D056804:Biofuels; D018533:Biomass; D019149:Bioreactors; D002708:Chlorella / Q000254:growth & development / Q000378:metabolism*; D052836:Heterotrophic Processes; D006863:Hydrogen-Ion Concentration; D008055:Lipids / Q000096:biosynthesis*; D058086:Microalgae
chemical_list: D056804:Biofuels; D008055:Lipids
keywords: Continuous fermentation; High biomass productivity; High cell density; High lipid productivity; Microalgae