title: Generation of Mitochondrial-nuclear eXchange Mice via Pronuclear Transfer.
abstract: The mitochondrial paradigm for common disease proposes that mitochondrial DNA (mtDNA) sequence variation can contribute to disease susceptibility and progression. To test this concept, we developed the Mitochondrial-nuclear eXchange (MNX) model, in which isolated embryonic pronuclei from one strain of species are implanted into an enucleated embryo of a different strain of the same species (e.g., C57BL/6 and C3H/HeN,Mus musculus),generating a re-constructed zygote harboring nuclear and mitochondrial genomes from different strains. Two-cell embryos are transferred to the ostia of oviducts in CD-1 pseudopregnant mice and developed to term. Nuclear genotype and mtDNA haplotype are verified in offspring, and females selected as founders for desired MNX colonies. By utilizing MNX models, many new avenues for thein vivostudy for mitochondrial and nuclear genetics, or mito-Mendelian genetics, are now possible.
authors.affiliation: Department of Genetics, University of Alabama, Birmingham, USA.
authors.forename: Robert A
authors.initials: RA
authors.lastname: Kesterson
authors.affiliation: Department of Genetics, University of Alabama, Birmingham, USA.
authors.forename: Larry W
authors.initials: LW
authors.lastname: Johnson
authors.affiliation: Department of Genetics, University of Alabama, Birmingham, USA.
authors.forename: Laura J
authors.initials: LJ
authors.lastname: Lambert
authors.affiliation: Department of Pathology, University of Kansas Medical Center, Kansas City, USA.
authors.forename: Jay L
authors.initials: JL
authors.lastname: Vivian
authors.affiliation: Department of Cancer Biology, University of Kansas Cancer Center, Kansas City, USA.
authors.forename: Danny R
authors.initials: DR
authors.lastname: Welch
authors.affiliation: Division of Molecular and Cellular Pathology, University of Alabama, Birmingham, USA.
authors.forename: Scott W
authors.initials: SW
authors.lastname: Ballinger