title: [The relationships of polychlorinated dibenzo-p-dioxins and dibenzofurans (PCDD/Fs) inhalational dose and oxidative damage in foundry workers].
abstract: Objective:To investigate the potential dose-response relationship between PCDD/Fs inhalational exposure and oxidative damage to DNA and lipid in foundry workers.Methods:The participants were divided into three groups, including exposure group, administrator group and control group. The concentrations of polychlorinated dibenzo-p-dioxins and dibenzofurans (PCDD/Fs) in air environment were determined by isotope dilution high resolution capillary column gas chromatography/high resolution mass spectrometry (HRGC-HRMS). The inhalational dose of PCDD/Fs were evaluated by Houmen's model. Urinary 8-OHdG was analyzed by high performance liquid chromatography-electrochemical detector (HPLC-ECD). Urinary 8-iso-PGF2ɑ were determined by ELISA.Results:The PCDD/Fs environmental concentrations of three groups (pg·TEQ/m) were 0.21±0.08, 0.03±0.01, 0.0012±0.0007 (P<0.05) , respectively. The inhalational dose of exposure group (92.54 (32.90~264.49) fg·TEQ/kg·d) was 5.4 folds higher than the administrator group, and was 201 times more than control group. The levels of urinary 8-OHdG (nmol/L crea) of three groups were 3.25 (1.07~15.36) , 2.72 (0.75~16.07) , 2.17 (0.38~17.07) (P<0.05) and the levels of urinary 8-iso-PGF2ɑ (nmol/L crea) were 19.11 (9.33~76.50) , 20.95 (8.40~79.31) , 11.48 (4.85~37.80) (P<0.05) , respectively. The multiple linear regression analysis revealed that there were significantly increased trend for levels of urinary 8-OHdG and 8-iso-PGF2ɑ as PCDD/Fs exposure levels increased, after adjustment for age, sex, smoking statue, alcohol use and BMI (P<0.01).Conclusion:There was a dose-response relationship between PCDD/Fs inhalational exposure and oxidative damage in foundry workers.
authors.affiliation: *Department of Occupational and Environmental Health and Ministry of Education Key Lab of Environment and Health, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
authors.forename: J T
authors.initials: JT
authors.lastname: He
authors.forename: S K
authors.initials: SK
authors.lastname: Zhang
authors.forename: M
authors.initials: M
authors.lastname: Zhao
authors.forename: Z
authors.initials: Z
authors.lastname: Zhang
authors.forename: T M
authors.initials: TM
authors.lastname: Shi
authors.forename: W H
authors.initials: WH
authors.lastname: Chen
mesh_terms: D000072318:Dibenzofurans; D015237:Dinoprost / Q000031:analogs & derivatives; D004147:Dioxins; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D019570:Inhalation Exposure; D018384:Oxidative Stress; D000072317:Polychlorinated Dibenzodioxins
chemical_list: D000072318:Dibenzofurans; D004147:Dioxins; D000072317:Polychlorinated Dibenzodioxins; C075750:8-epi-prostaglandin F2alpha; D015237:Dinoprost