title: Polyphosphoric acid-induced perylene probe self-assembly and label-free fluorescence turn-on detection of alkaline phosphatase.
abstract: A label-free fluorescence turn-on strategy for alkaline phosphatase (ALP) detection was established based on its enzymatic catalyzed hydrolysis of polyphosphoric acid (PPA, an anionic polymer) that had been utilized for aggregation with our homemade positively charged perylene derivative (Probe-1) via noncovalent interactions. The disaggregation caused turn-on fluorescence signal which was recovered by the released Proble-1 molecules whose original strong fluorescence in an aqueous buffer solution had been quenched due to their previous aggregation induced by PPA. Such method presents its great advantages of free labeling, convenience and simplicity, cost effectiveness, high selectivity, and high sensitivity, with a detection limit of 0.5 mU/mL of ALP. Graphical Abstract A label-free fluorescence turn-on strategy for alkaline phosphatase based on its enzymatic catalyzed hydrolysis of polyphosphoric acid that had been utilized for aggregation with our homemade positively charged perylene derivative via noncovalent interactions.
authors.affiliation: School of Chemical & Environmental Engineering, Changchun University of Science and Technology, Changchun, Jilin, 130022, China.|State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin, 130022, China.|University of Chinese Academy of Sciences, Beijing, 100049, China.
authors.forename: Wenping
authors.initials: W
authors.lastname: Zhang
authors.affiliation: School of Chemical & Environmental Engineering, Changchun University of Science and Technology, Changchun, Jilin, 130022, China. gaoying@cust.edu.cn.
authors.forename: Ying
authors.initials: Y
authors.lastname: Gao
authors.affiliation: State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin, 130022, China. liyongxin@ciac.ac.cn.|University of Chinese Academy of Sciences, Beijing, 100049, China. liyongxin@ciac.ac.cn.
authors.forename: Yongxin
authors.initials: Y
authors.lastname: Li
authors.affiliation: State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin, 130022, China.|University of Chinese Academy of Sciences, Beijing, 100049, China.
authors.forename: Qingfeng
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin, 130022, China.|University of Chinese Academy of Sciences, Beijing, 100049, China.
authors.forename: Zhenzhen
authors.initials: Z
authors.lastname: Hu
authors.affiliation: State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin, 130022, China.|University of Chinese Academy of Sciences, Beijing, 100049, China.
authors.forename: Yunyi
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin, 130022, China.|University of Chinese Academy of Sciences, Beijing, 100049, China.
authors.forename: Ejaz
authors.initials: E
authors.lastname: Hussain
authors.affiliation: School of Chemical & Environmental Engineering, Changchun University of Science and Technology, Changchun, Jilin, 130022, China.
authors.forename: Xiuyun
authors.initials: X
authors.lastname: Yang
authors.affiliation: Department of Chemistry and Bioscience, Aalborg University, 9220, Aalborg East, Denmark. yu@bio.aau.dk.|Sino-Danish Center for Education and Research, Aarhus University, 8000, Aarhus, Denmark. yu@bio.aau.dk.
authors.forename: Donghong
authors.initials: D
authors.lastname: Yu
authors.affiliation: State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin, 130022, China. congyu@ciac.ac.cn.|University of Chinese Academy of Sciences, Beijing, 100049, China. congyu@ciac.ac.cn.
authors.forename: Cong
authors.initials: C
authors.lastname: Yu
mesh_terms: D000469:Alkaline Phosphatase / Q000032:analysis*; D010569:Perylene / Q000737:chemistry*; D010756:Phosphoric Acids / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry*; D013050:Spectrometry, Fluorescence
chemical_list: D010756:Phosphoric Acids; D011108:Polymers; D010569:Perylene; C047269:polyphosphoric acid; D000469:Alkaline Phosphatase
keywords: Aggregation; Alkaline phosphatase; Fluorescence; Perylene; Polyphosphoric acid; Small molecule probe