title: Analyzing Cell Wall Elasticity After Hormone Treatment: An Example Using Tobacco BY-2 Cells and Auxin.
abstract: Atomic force microscopy, and related nano-indentation techniques, is a valuable tool for analyzing the elastic properties of plant cell walls as they relate to changes in cell wall chemistry, changes in development, and response to hormones. Within this chapter I will describe a method for analyzing the effect of the phytohormone auxin on the cell wall elasticity of tobacco BY-2 cells. This general method may be easily altered for different experimental systems and hormones of interest.
authors.affiliation: The Sainsbury Laboratory, University of Cambridge, Bateman Street, Cambridge, CB2 1LR, UK. siobhan.braybrook@slcu.cam.ac.uk.
authors.forename: Siobhan A
authors.initials: SA
authors.lastname: Braybrook
mesh_terms: D002460:Cell Line; D002473:Cell Wall / Q000187:drug effects* / Q000502:physiology*; D055119:Elastic Modulus / Q000187:drug effects / Q000502:physiology; D004548:Elasticity / Q000187:drug effects* / Q000502:physiology*; D007210:Indoleacetic Acids / Q000494:pharmacology*; D018625:Microscopy, Atomic Force / Q000379:methods; D010937:Plant Growth Regulators / Q000494:pharmacology*; D014026:Nicotiana / Q000187:drug effects / Q000502:physiology*
chemical_list: D007210:Indoleacetic Acids; D010937:Plant Growth Regulators
keywords: Atomic force microscopy; Auxin; Cell growth; Cell wall; Elasticity