title: Development of highly transferable microsatellites forPanax ginseng(Araliaceae) using whole-genome data.
abstract: OBJECTIVE
  Highly transferable expressed sequence tag (EST) microsatellites were developed forPanax ginseng(Araliaceae), one of the most celebrated traditional Chinese medicines and an endangered species in East Asia, using whole-genome data.
  
  
  RESULTS
  Twenty-one EST microsatellites were characterized from next-generation sequencing and were composed of di- and trinucleotide repeats. Polymorphisms and genetic diversity were evaluated for 45 accessions of three ginseng landraces. The number of alleles for each locus ranged from one to five among the landraces, and the polymorphism information content varied from 0.0000 to 0.6450. These microsatellites were also tested for congeneric amplification withP. notoginseng,P. stipuleanatus,P. quinquefolius,P. bipinnatifidus, and the closely related speciesAralia elata.
  
  
  CONCLUSIONS
  These novel EST-derived microsatellite markers will facilitate further population genetic studies of the generaPanaxandAralia.
authors.affiliation: Key Laboratory of Molecular Epigenetics of the Ministry of Education, Northeast Normal University, Changchun 130024, People's Republic of China.
authors.forename: Peng
authors.initials: P
authors.lastname: Jiang
authors.affiliation: Museum of Natural History, Northeast Normal University, Changchun 130117, People's Republic of China.
authors.forename: Feng-Xue
authors.initials: FX
authors.lastname: Shi
authors.affiliation: Key Laboratory of Molecular Epigenetics of the Ministry of Education, Northeast Normal University, Changchun 130024, People's Republic of China.
authors.forename: Ya-Ling
authors.initials: YL
authors.lastname: Li
authors.affiliation: Key Laboratory of Molecular Epigenetics of the Ministry of Education, Northeast Normal University, Changchun 130024, People's Republic of China.
authors.forename: Bao
authors.initials: B
authors.lastname: Liu
authors.affiliation: Key Laboratory of Molecular Epigenetics of the Ministry of Education, Northeast Normal University, Changchun 130024, People's Republic of China; Department of Biology, Washington University, St. Louis, Missouri 63130 USA.
authors.forename: Lin-Feng
authors.initials: LF
authors.lastname: Li
keywords: Araliaceae; Panax ginseng; microsatellite; polyploidy; traditional Chinese medicine