title: OsACOS12, an orthologue of Arabidopsis acyl-CoA synthetase5, plays an important role in pollen exine formation and anther development in rice.
abstract: Sporopollenin is a major component of the pollen exine pattern. In Arabidopsis, acyl-CoA synthetase5 (ACOS5) is involved in sporopollenin precursor biosynthesis. In this study, we identified its orthologue, OsACOS12, in rice (Oryza sativa) and compared the functional conservation of ACOS in rice to Arabidopsis.
  
  
  
  Sequence analysis showed that OsACOS12 shares 63.9 % amino acid sequence identity with ACOS5. The osacos12 mutation caused by a pre-mature stop codon in LOC_Os04g24530 exhibits defective sexine resulting in a male sterile phenotype in rice. In situ hybridization shows that OsACOS12 is expressed in tapetal cells and microspores at the transcript level. The localization of OsACOS12-GFP demonstrated that OsACOS12 protein is accumulated in tapetal cells and anther locules. OsACOS12 driven by the ACOS5 promoter could partially restore the male fertility of the acos5 mutant in Arabidopsis.
  
  
  
  OsACOS12 is an orthologue of ACOS5 that is essential for sporopollenin synthesis in rice. ACOS5 and OsACOS12 are conserved for pollen wall formation in monocot and dicot species.
authors.affiliation: College of Life and Environment Sciences, Shanghai Normal University, 100 Guilin Road, Shanghai, 200234, China.
authors.forename: Yueling
authors.initials: Y
authors.lastname: Li
authors.affiliation: College of Life and Environment Sciences, Shanghai Normal University, 100 Guilin Road, Shanghai, 200234, China.
authors.forename: Dandan
authors.initials: D
authors.lastname: Li
authors.affiliation: College of Life and Environment Sciences, Shanghai Normal University, 100 Guilin Road, Shanghai, 200234, China.
authors.forename: Zongli
authors.initials: Z
authors.lastname: Guo
authors.affiliation: College of Life and Environment Sciences, Shanghai Normal University, 100 Guilin Road, Shanghai, 200234, China.
authors.forename: Qiangsheng
authors.initials: Q
authors.lastname: Shi
authors.affiliation: College of Life and Environment Sciences, Shanghai Normal University, 100 Guilin Road, Shanghai, 200234, China.
authors.forename: Shuangxi
authors.initials: S
authors.lastname: Xiong
authors.affiliation: College of Life and Environment Sciences, Shanghai Normal University, 100 Guilin Road, Shanghai, 200234, China.
authors.forename: Cheng
authors.initials: C
authors.lastname: Zhang
authors.affiliation: College of Life and Environment Sciences, Shanghai Normal University, 100 Guilin Road, Shanghai, 200234, China. zhujun78@shnu.edu.cn.
authors.forename: Jun
authors.initials: J
authors.lastname: Zhu
authors.affiliation: College of Life and Environment Sciences, Shanghai Normal University, 100 Guilin Road, Shanghai, 200234, China. znyang@shnu.edu.cn.
authors.forename: Zhongnan
authors.initials: Z
authors.lastname: Yang
mesh_terms: D017360:Arabidopsis / Q000201:enzymology / Q000235:genetics / Q000254:growth & development; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D003066:Coenzyme A Ligases / Q000235:genetics / Q000378:metabolism*; D035264:Flowers / Q000201:enzymology / Q000235:genetics / Q000254:growth & development; D018506:Gene Expression Regulation, Plant; D012275:Oryza / Q000201:enzymology / Q000235:genetics / Q000254:growth & development; D011058:Pollen / Q000201:enzymology / Q000235:genetics / Q000254:growth & development
chemical_list: D029681:Arabidopsis Proteins; C542001:ACOS5 protein, Arabidopsis; D003066:Coenzyme A Ligases
keywords: Anther cuticle; Male sterility; Oryza sativa; OsACOS12; Pollen exine