title: Transcriptome-Based Discovery of Fusarium graminearum Stress Responses to FgHV1 Infection.
abstract: Fusarium graminearum hypovirus 1 (FgHV1), which is phylogenetically related to Cryphonectria hypovirus 1 (CHV1), is a virus in the familyHypoviridaethat infects the plant pathogenic fungusF. graminearum. Although hypovirus FgHV1 infection does not attenuate the virulence of the host (hypovirulence), it results in defects in mycelial growth and spore production. We now report that the vertical transmission rate of FgHV1 through asexual spores reached 100%. Using RNA deep sequencing, we performed genome-wide expression analysis to reveal phenotype-related genes with expression changes in response to FgHV1 infection. A total of 378 genes were differentially expressed, suggesting that hypovirus infection causes a significant alteration of fungal gene expression. Nearly two times as many genes were up-regulated as were down-regulated. A differentially expressed gene enrichment analysis identified a number of important pathways. Metabolic processes, the ubiquitination system, and especially cellular redox regulation were the most affected categories inF. graminearumchallenged with FgHV1. The p20, encoded by FgHV1 could induce H₂O₂ accumulation and hypersensitive response inNicotiana benthamianaleaves. Moreover, hypovirus FgHV1 may regulate transcription factors and trigger the RNA silencing pathway inF. graminearum.
authors.affiliation: State Key Laboratory for Biology of Plant Disease and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Science, Beijing 100081, China. yuxinren2006@163.com.|Walloon Centre of Industrial Biology, Gembloux Agro-Bio Tech, University of Liège, Passage des Déportés, 2, Gembloux 5030, Belgium. yuxinren2006@163.com.
authors.forename: Shuangchao
authors.initials: S
authors.lastname: Wang
authors.affiliation: State Key Laboratory for Biology of Plant Disease and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Science, Beijing 100081, China. jingzezhang0820@gmail.com.
authors.forename: Jingze
authors.initials: J
authors.lastname: Zhang
authors.affiliation: State Key Laboratory for Biology of Plant Disease and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Science, Beijing 100081, China. li_pengfei2014@163.com.
authors.forename: Pengfei
authors.initials: P
authors.lastname: Li
authors.affiliation: State Key Laboratory for Biology of Plant Disease and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Science, Beijing 100081, China. qiudewen@caas.cn.
authors.forename: Dewen
authors.initials: D
authors.lastname: Qiu
authors.affiliation: State Key Laboratory for Biology of Plant Disease and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Science, Beijing 100081, China. guolihua72@yahoo.com.
authors.forename: Lihua
authors.initials: L
authors.lastname: Guo
mesh_terms: D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism; D000067148:Fungal Viruses / Q000502:physiology*; D005670:Fusarium / Q000235:genetics / Q000378:metabolism* / Q000821:virology; D015966:Gene Expression Regulation, Fungal; D063990:Gene Ontology; D053263:Gene Regulatory Networks; D005800:Genes, Fungal; D058977:Molecular Sequence Annotation; D012328:RNA Viruses / Q000502:physiology*; D017423:Sequence Analysis, RNA; D013312:Stress, Physiological; D059467:Transcriptome
chemical_list: D005656:Fungal Proteins
keywords: Fusarium graminearum; Fusarium graminearum hypovirus 1; cellular redox regulation; stress responses; transcriptome