title: Effect of chicken leptin recptor short hairpin RNA on expression of JAK2, STAT3, SOCS3 and CPT1 genes in chicken preadipocytes.
abstract: In this study, we detect depressive effect on leptin receptor (LEPR) by LEPR-specific short hairpin RNA (shRNA) expression plasmids in chicken preadipocytes, and effect on messenger RNA (mRNA) expression levels of genes related to signal transduction, including JAK2, STAT3, SOCS3 as well as CPT1, which is associated with fatty acid metabolism. shRNA expression vectors targeting LEPR were constructed and transfected into chicken preadipocytes. The transfection efficiency was evaluated by fluorescence microscopy. Real-time PCR was used to detect its effect on mRNA expression levels of JAK2, STAT3, SOCS3 and CPT1. Results showed that LEPR mRNA was knocked down by 99% (P < 0.01) after transfection for 72 h. In the knockdown preadipocytes, the mRNA levels of JAK2 and CPT1 were down-regulated by 47.56% (P < 0.01) and 42.26% (P < 0.05), respectively; while expression of STAT3 and SOCS3 increased 7.72-fold (P < 0.01), 1.71-fold (P < 0.01), respectively. It is concluded that knockdown of LEPR influences mRNA expression of its down-stream genes, suggesting that chicken LEPR play a certain role in regulating genes in the complicated gene network of preadipocytes.
authors.affiliation: School of Life Science, Anhui University, Hefei, China.|College of Animal Science and Technology, Shihezi University, Shihezi, China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Ban
authors.affiliation: Institute of Animal Science and Veterinary Medicine, Anhui Academy of Agricultural Sciences, Hefei, China.|College of Animal Science and Technology, Shihezi University, Shihezi, China.
authors.forename: Wenqiao
authors.initials: W
authors.lastname: Hui
authors.affiliation: School of Life Science, Anhui University, Hefei, China.
authors.forename: Fei
authors.initials: F
authors.lastname: Cheng
authors.affiliation: School of Life Science, Anhui University, Hefei, China.
authors.forename: Dahai
authors.initials: D
authors.lastname: Liu
authors.affiliation: College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou, China.|College of Animal Science and Technology, Shihezi University, Shihezi, China.
authors.forename: Xiaojun
authors.initials: X
authors.lastname: Liu
mesh_terms: D017667:Adipocytes / Q000378:metabolism*; D000818:Animals; D002334:Carnitine O-Palmitoyltransferase / Q000235:genetics* / Q000378:metabolism*; D002478:Cells, Cultured; D002645:Chickens / Q000235:genetics* / Q000378:metabolism*; D005227:Fatty Acids / Q000378:metabolism; D015870:Gene Expression; D053614:Janus Kinase 2 / Q000235:genetics* / Q000378:metabolism*; D010957:Plasmids; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D034741:RNA, Small Interfering / Q000235:genetics* / Q000502:physiology*; D054411:Receptors, Leptin / Q000502:physiology*; D050796:STAT3 Transcription Factor / Q000235:genetics* / Q000378:metabolism*; D015398:Signal Transduction / Q000235:genetics; D000071223:Suppressor of Cytokine Signaling 3 Protein / Q000235:genetics* / Q000378:metabolism*; D014162:Transfection
chemical_list: D005227:Fatty Acids; D012333:RNA, Messenger; D034741:RNA, Small Interfering; D054411:Receptors, Leptin; D050796:STAT3 Transcription Factor; D000071223:Suppressor of Cytokine Signaling 3 Protein; D002334:Carnitine O-Palmitoyltransferase; D053614:Janus Kinase 2
keywords: LEPR; RNAi; chicken; preadipocytes