title: Bio-based production of fuels and industrial chemicals by repurposing antibiotic-producing type I modular polyketide synthases: opportunities and challenges.
abstract: Complex polyketides comprise a large number of natural products that have broad application in medicine and agriculture. They are produced in bacteria and fungi from large enzyme complexes named type I modular polyketide synthases (PKSs) that are composed of multifunctional polypeptides containing discrete enzymatic domains organized into modules. The modular nature of PKSs has enabled a multitude of efforts to engineer the PKS genes to produce novel polyketides of predicted structure. We have repurposed PKSs to produce a number of short-chain mono- and di-carboxylic acids and ketones that could have applications as fuels or industrial chemicals.
authors.affiliation: Biological Systems and Engineering Division, Lawrence Berkeley National Laboratory, Berkeley, CA, USA.
authors.forename: Satoshi
authors.initials: S
authors.lastname: Yuzawa
authors.affiliation: Biological Systems and Engineering Division, Lawrence Berkeley National Laboratory, Berkeley, CA, USA.|QB3 Institute, University of California, Berkeley, CA, USA.|Joint BioEnergy Institute, Emeryville, CA, USA.|Department of Bioengineering, University of California, Berkeley, CA, USA.|Department of Chemical and Biomolecular Engineering, University of California, Berkeley, CA, USA.|Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Hørsholm, Denmark.
authors.forename: Jay D
authors.initials: JD
authors.lastname: Keasling
authors.affiliation: QB3 Institute, University of California, Berkeley, CA, USA.
authors.forename: Leonard
authors.initials: L
authors.lastname: Katz
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000096:biosynthesis*; D001419:Bacteria / Q000378:metabolism; D056804:Biofuels; D002617:Chemical Industry; D006801:Humans; D048630:Polyketide Synthases / Q000235:genetics / Q000378:metabolism*; D061065:Polyketides
chemical_list: D000900:Anti-Bacterial Agents; D056804:Biofuels; D061065:Polyketides; D048630:Polyketide Synthases