title: Preparation of magnetic molecularly imprinted polymers by atom transfer radical polymerization for the rapid extraction of avermectin from fish samples.
abstract: A novel and highly efficient approach to obtain magnetic molecularly imprinted polymers is described to detect avermectin in fish samples. The magnetic molecularly imprinted polymers were synthesized by surface imprinting polymerization using magnetic multiwalled carbon nanotubes as the support materials, atom transfer radical polymerization as the polymerization method, avermectin as template, acrylamide as functional monomer, and ethylene glycol dimethacrylate as crosslinker. The characteristics of the magnetic molecularly imprinted polymers were assessed by using transmission electron microscopy, Fourier transform infrared spectroscopy, X-ray photoelectron spectroscopy, vibrating sample magnetometry, X-ray diffraction, and thermogravimetric analysis. The binding characteristics of magnetic molecularly imprinted polymers were researched through isothermal adsorption experiment, kinetics adsorption experiment, and the selectivity experiment. Coupled with ultra high performance liquid chromatography and tandem mass spectrometry, the extraction conditions of the magnetic molecularly imprinted polymers as adsorbents for avermectin were investigated in detail. The recovery of avermectin was 84.2-97.0%, and the limit of detection was 0.075 μg/kg. Relative standard deviations of intra- and inter-day precisions were in the range of 1.7-2.9% and 3.4-5.6%, respectively. The results demonstrated that the extraction method not only has high selectivity and accuracy, but also is convenient for the determination of avermectin in fish samples.
authors.affiliation: Department of Chemistry, College of Science, Northeast Forestry University, Harbin, China.
authors.forename: Xiaoxiao
authors.initials: X
authors.lastname: You
authors.affiliation: Heilongjiang River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Harbin, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Gao
authors.affiliation: Heilongjiang River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Harbin, China.
authors.forename: Dongli
authors.initials: D
authors.lastname: Qin
authors.affiliation: Department of Chemistry, College of Science, Northeast Forestry University, Harbin, China.
authors.forename: Ligang
authors.initials: L
authors.lastname: Chen
mesh_terms: D000327:Adsorption; D000818:Animals; D002623:Chemistry Techniques, Analytical / Q000379:methods*; D005399:Fishes; D007559:Ivermectin / Q000031:analogs & derivatives* / Q000032:analysis / Q000302:isolation & purification; D057230:Limit of Detection; D054802:Molecular Imprinting; D018482:Muscle, Skeletal / Q000737:chemistry*; D037742:Nanotubes, Carbon / Q000737:chemistry; D058105:Polymerization; D011108:Polymers / Q000138:chemical synthesis*
chemical_list: D037742:Nanotubes, Carbon; D011108:Polymers; D007559:Ivermectin; C019264:avermectin
keywords: atom transfer radical polymerization; carbon nanotubes; magnetic properties; molecularly imprinted polymers; specific recognition