title: A New MicroRNA Expression Signature for Cervical Cancer.
abstract: Cervical cancer is the second most common cancer among women worldwide. The potential of microRNAs as novel biomarkers in cervical cancer is growing.
  
  
  
  In this study, we investigated the functions and targets of miR-466 in cervical cancer tissues.
  
  
  
  Fresh cervical tissues were obtained from 157 patients with cervical cancer, cervical intraepithelial neoplasia (CIN), and healthy controls, and the tissues were immediately frozen in liquid nitrogen until use. The RNA was extracted and quantitative real-time polymerase chain reaction (PCR) was performed.
  
  
  
  A total of 157 participants were summarized, including 56 patients with cervical cancer, 60 patients with CIN, and 49 healthy controls. The expression levels of miR-466 in cervical cancers (0.68) were higher than that in healthy controls (0.082) (P < 0.01). The average fold changes of miR-466 in the patients with CIN group and people group were 0.28 and 0.082, respectively (P < 0.01). It was a statistically significant difference in patients with lymph node involvement (P = 0.022). However, the expression of miR-466 was not correlated with International Federation of Gynecology and Obstetrics stages, tumor size, or vascular invasion (P = 0.506, P = 0.667, and P = 0.108, respectively).
  
  
  
  Our results indicate that the aberrant expression of miR-466 is closely associated with the occurrence and development of cervical cancer.
authors.affiliation: *Department of Clinical Laboratory, Affiliated Cancer Hospital of Zhengzhou University, Henan Cancer Hospital; †Department of Clinical Laboratory, the Second Affiliated Hospital of Zhengzhou University; and ‡Department of Obstetrics and Gynecology, Affiliated Cancer Hospital of Zhengzhou University, Henan Cancer Hospital, Zhengzhou, Henan, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Sun
authors.forename: Yong
authors.initials: Y
authors.lastname: Shen
authors.forename: Jiao-Mei
authors.initials: JM
authors.lastname: Gong
authors.forename: Li-Li
authors.initials: LL
authors.lastname: Zhou
authors.forename: Jia-He
authors.initials: JH
authors.lastname: Sheng
authors.forename: Fu-Jiao
authors.initials: FJ
authors.lastname: Duan
mesh_terms: D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000096:biosynthesis* / Q000235:genetics*; D016022:Case-Control Studies; D005260:Female; D006801:Humans; D035683:MicroRNAs / Q000096:biosynthesis* / Q000235:genetics; D008875:Middle Aged; D012334:RNA, Neoplasm / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D002583:Uterine Cervical Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D002578:Uterine Cervical Dysplasia / Q000235:genetics* / Q000378:metabolism / Q000473:pathology
chemical_list: D014408:Biomarkers, Tumor; C585904:MIRN466 microRNA, human; D035683:MicroRNAs; D012334:RNA, Neoplasm