title: The expression of HoxB5 and SPC in neonatal rat lung after exposure to fluoxetine.
abstract: OBJECTIVE
  Approximately 10% of pregnant women suffer from pregnancy-associated depression. Fluoxetine, as a selective serotonin reuptake inhibitor, is being employed as a therapy for depressive disorders. The present study aimed to determine the effects of fluoxetine on neonatal lung development.
  
  
  METHODS
  Thirty pregnant Wistar rats (weighing 200-250 g) were treated daily with 7 mg/kg fluoxetine from gestation day 0 to gestation day 21, via gavage. The control group received a similar volume of distilled water only. Following delivery, the newborns and their lungs were immediately weighed in both of the groups. The right lung was fixed for histological assessments while the left lung was used for evaluation of the expression of SPC and HoxB5 by the real-time polymerase chain reaction method.
  
  
  RESULTS
  Results have indicated that even though the body weight and the number of neonatal rats in both groups were the same, the lung weight of neonates exposed to fluoxetine was significantly different compared to the control group (P<0.05). Expression of both genes was increased, nonetheless, only elevation of HoxB5 was significant (P<0.05). Histological studies demonstrated that lung tissue in the fluoxetine treatment group morphologically appears to be similar to the pseudoglandular phase, whereas the control group lungs experienced more development.
  
  
  CONCLUSIONS
  According to the upregulated expression of HoxB5 concerning histological findings, results of the present study showed that fluoxetine can influence lung growth and may in turn lead to delay in lung development. So establishment of studies to identify the effects of antidepressant drugs during pregnancy is deserved.
authors.affiliation: Department of Biology, Payame Noor University, Isfahan, Iran.
authors.forename: Razieh
authors.initials: R
authors.lastname: Taghizadeh
authors.affiliation: Department of Anatomy, Rafsanjan University of Medical Sciences, Rafsanjan, Iran.
authors.forename: Zahra
authors.initials: Z
authors.lastname: Taghipour
authors.affiliation: Department of Biology, Payame Noor University, Isfahan, Iran.
authors.forename: Akbar
authors.initials: A
authors.lastname: Karimi
authors.affiliation: Department of Physiology, Rafsanjan University of Medical Sciences, Rafsanjan, Iran.
authors.forename: Ali
authors.initials: A
authors.lastname: Shamsizadeh
authors.affiliation: Department of Anatomy, Rafsanjan University of Medical Sciences, Rafsanjan, Iran.
authors.forename: Mohammad Mohsen
authors.initials: MM
authors.lastname: Taghavi
authors.affiliation: Department of Anatomy, Rafsanjan University of Medical Sciences, Rafsanjan, Iran.
authors.forename: Mahdi
authors.initials: M
authors.lastname: Shariati
authors.affiliation: Department of Anatomy, Rafsanjan University of Medical Sciences, Rafsanjan, Iran.
authors.forename: Ahmad
authors.initials: A
authors.lastname: Shabanizadeh
authors.affiliation: Department of Anatomy, Rafsanjan University of Medical Sciences, Rafsanjan, Iran.
authors.forename: Hamid Reza
authors.initials: HR
authors.lastname: Jafari Naveh
authors.affiliation: Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
authors.forename: Reza
authors.initials: R
authors.lastname: Bidaki
authors.affiliation: Rafsanjan University of Medical Sciences, Rafsanjan, Iran.
authors.forename: Fariba
authors.initials: F
authors.lastname: Aminzadeh
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D000928:Antidepressive Agents / Q000494:pharmacology*; D005260:Female; D005473:Fluoxetine / Q000494:pharmacology*; D008168:Lung / Q000502:physiology*; D011247:Pregnancy; D051381:Rats; D017208:Rats, Wistar; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods; D017367:Selective Serotonin Reuptake Inhibitors / Q000494:pharmacology*
chemical_list: D000928:Antidepressive Agents; D005473:Fluoxetine; D017367:Selective Serotonin Reuptake Inhibitors
keywords: HoxB5; SPC; fluoxetine; lung; rat