title: β-eudesmol suppresses allergic reactions via inhibiting mast cell degranulation.
abstract: The regulatory effect of β-eudesmol, which is an active constituent of Pyeongwee-San (KMP6), is evaluated for allergic reactions induced by mast cell degranulation. Phorbol 12-myristate 13-acetate (PMA) plus calcium ionophore A23187-stimulated human mast cell line, HMC-1 cells, and compound 48/80-stimulated rat peritoneal mast cells (RPMCs) are used as the in vitro models; mice models of systemic anaphylaxis, ear swelling, and IgE-dependent passive cutaneous anaphylaxis (PCA) are used as the in vivo allergic models. The results demonstrate that β-eudesmol suppressed the histamine and tryptase releases from the PMA plus calcium ionophore A23187-stimulated HMC-1 cells. β-eudesmol inhibits the expression and activity of histidine decarboxylase in the activated HMC-1 cells. In addition, β-eudesmol inhibits the levels of histamine and tryptase released from the compound 48/80-stimulated RPMCs. Furthermore, β-eudesmol decreases the intracellular calcium level in the activated RPMCs. β-eudesmol also decreases the compound 48/80-induced mortality and ear swelling response. β-eudesmol suppresses the serum levels of histamine, IgE, interleukin (IL)-1β, IL-4, IL-5, IL-6, IL-13, and vascular endothelial growth factor (VEGF) under PCA mice as well as PCA reactions. Therefore, the results from this study indicate the potential of β-eudesmol as an anti-allergic drug with respect to its pharmacological properties against mast cell-mediated allergic reactions.
authors.affiliation: Department of Pharmacology, College of Korean Medicine, Kyung Hee University, Seoul, Korea.
authors.forename: Na-Ra
authors.initials: NR
authors.lastname: Han
authors.affiliation: Department of Pharmacology, College of Korean Medicine, Kyung Hee University, Seoul, Korea.
authors.forename: Phil-Dong
authors.initials: PD
authors.lastname: Moon
authors.affiliation: Department of Pharmacology, College of Korean Medicine, Kyung Hee University, Seoul, Korea.
authors.forename: Ka-Jung
authors.initials: KJ
authors.lastname: Ryu
authors.affiliation: Department of Pharmaceutical Engineering and Inflammatory Disease Research Centre, Hoseo University, Asan, Chungnam, Korea.
authors.forename: Jae-Bum
authors.initials: JB
authors.lastname: Jang
authors.affiliation: Department of Pharmacology, College of Korean Medicine, Kyung Hee University, Seoul, Korea.
authors.forename: Hyung-Min
authors.initials: HM
authors.lastname: Kim
authors.affiliation: Department of Food Science & Technology and Research Institute for Basic Science, Hoseo University, Asan, Chungnam, Korea.
authors.forename: Hyun-Ja
authors.initials: HJ
authors.lastname: Jeong
mesh_terms: D000707:Anaphylaxis / Q000097:blood / Q000188:drug therapy* / Q000276:immunology / Q000473:pathology; D000818:Animals; D018926:Anti-Allergic Agents / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D015550:Cell Degranulation / Q000187:drug effects* / Q000276:immunology; D002460:Cell Line; D016207:Cytokines / Q000097:blood; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D006632:Histamine / Q000097:blood; D006801:Humans; D007073:Immunoglobulin E / Q000097:blood; D008407:Mast Cells / Q000187:drug effects* / Q000276:immunology; D051379:Mice; D010323:Passive Cutaneous Anaphylaxis / Q000187:drug effects*; D051381:Rats; D045787:Sesquiterpenes, Eudesmane / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use
chemical_list: D018926:Anti-Allergic Agents; D016207:Cytokines; D045787:Sesquiterpenes, Eudesmane; D007073:Immunoglobulin E; C051082:beta-eudesmol; D006632:Histamine
keywords: anaphylaxis; degranulation; ear swelling; histamine; mast cells; β-eudesmol