title: STARD13 promotes hepatocellular carcinoma apoptosis by acting as a ceRNA for Fas.
abstract: OBJECTIVE
  To study the roles of STARD13 in cellular apoptosis of hepatocellular carcinoma (HCC).
  
  
  RESULTS
  Quantitative real-time PCR and immunohistochemistry analyses showed that the expression levels of STARD13 and Fas were lower in clinical HCC tissues than in normal tissues and were positively correlated, which is consistent with the results analyzed by The Cancer Genome Atlas (TCGA) data. Patients with higher STARD13 or Fas expression levels had longer overall survival. Additionally, STARD13 3'-UTR enhanced cellular apoptosis and the 3'-UTRs of STARD13 and Fas were predicted to harbor nine similar miRNA binding sites. And STARD13 3'-UTR promoted Fas expression in a 3'-UTR- and miRNA-dependent way and increased the sensitivity of HCC cells to chemotherapy. Importantly, the coding sequence of STARD13 did not increase Fas expression.
  
  
  CONCLUSIONS
  STARD13 3'-UTR promotes HCC apoptosis through acting as a ceRNA for Fas.
authors.affiliation: Department of Gastroenterology, Huangshi Central Hospital, The Affiliated Hospital of Hubei Polytechnic University, Huangshi, 435000, China.
authors.forename: Hai
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Department of Gastroenterology, Huangshi Central Hospital, The Affiliated Hospital of Hubei Polytechnic University, Huangshi, 435000, China.
authors.forename: Fang
authors.initials: F
authors.lastname: Wang
authors.affiliation: Department of Gastroenterology, Huangshi Central Hospital, The Affiliated Hospital of Hubei Polytechnic University, Huangshi, 435000, China. yahua_hu@163.com.
authors.forename: Yahua
authors.initials: Y
authors.lastname: Hu
mesh_terms: D017209:Apoptosis / Q000235:genetics / Q000502:physiology; D006528:Carcinoma, Hepatocellular / Q000235:genetics / Q000378:metabolism*; D002453:Cell Cycle / Q000235:genetics / Q000502:physiology; D045744:Cell Line, Tumor; D020690:GTPase-Activating Proteins; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics; D056945:Hep G2 Cells; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics* / Q000378:metabolism*; D035683:MicroRNAs / Q000235:genetics; D012313:RNA / Q000235:genetics*; D062085:RNA, Long Noncoding / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics; D025521:Tumor Suppressor Proteins / Q000235:genetics / Q000378:metabolism*; D019014:fas Receptor / Q000235:genetics*
chemical_list: D020690:GTPase-Activating Proteins; D035683:MicroRNAs; D062085:RNA, Long Noncoding; D012333:RNA, Messenger; C473607:STARD13 protein, human; D025521:Tumor Suppressor Proteins; D019014:fas Receptor; D012313:RNA
keywords: 3′-UTR; Apoptosis; Fas; Hepatocellular carcinoma; STARD13; Tumor suppression; ceRNA