title: Purification of Bioactive Lipopeptides Produced byBacillus subtilisStrain BIA.
abstract: Bacillus subtilisstrain BIA was used for the production of bioactive lipopeptides. Different extraction and purification methods were assayed as liquid-liquid extraction, and acid and ammonium sulfate precipitation followed by TLC, SPE, and gel filtration. Active fractions were further purified using RP-HPLC. The molecular mass of the purified product from HPLC was determined through Tris-Tricine SDS-PAGE and MALDI-TOF-MS. The results revealed thatBacillus subtilisstrain BIA produced surfactin and iturin like compounds. Coproduction of surfactin and iturin like compounds by this strain is a remarkable trait for a potential biocontrol agent. This paper also includeds techniques that have been developed for the optimal and convenient extraction of bioactive lipopeptides from microbial origin.
authors.affiliation: Department of Pharmacognosy, Institute of Pharmaceutical Sciences, University of Graz, Universitaetsplatz 4/I, 8010 Graz, Austria.
authors.forename: Muaaz
authors.identifier: 0000-0001-9087-6863
authors.initials: M
authors.lastname: Alajlani
authors.affiliation: Department of Microbiology and Molecular Genetics, Faculty of Life Sciences, University of the Punjab, Lahore, Pakistan.
authors.forename: Abid
authors.initials: A
authors.lastname: Shiekh
authors.affiliation: Department of Microbiology and Molecular Genetics, Faculty of Life Sciences, University of the Punjab, Lahore, Pakistan ; Department of Microbiology and Molecular Genetics, Faculty of Life Sciences, The Women University Multan, Multan, Pakistan.
authors.forename: Shahida
authors.initials: S
authors.lastname: Hasnain
authors.affiliation: Department of Pharmacognosy, Institute of Pharmaceutical Sciences, University of Graz, Universitaetsplatz 4/I, 8010 Graz, Austria.
authors.forename: Adelheid
authors.initials: A
authors.lastname: Brantner
keywords: Gel-filtration; Lipopeptides; MALDI–TOF-MS; Purification; RP-HPLC; TLC