title: The transcription factor RFX5 is a transcriptional activator of the TPP1 gene in hepatocellular carcinoma.
abstract: Regulatory factor X-5 (RFX5) was previously characterized as an essential and highly specific regulator of major histocompatibility class II (MHCII) gene expression in the immune system. We found that RFX5 is significantly upregulated in hepatocellular carcinoma (HCC) tumors and cell lines compared with non-tumor tissues in mRNA expression levels, but it fails to induce the expression of MHCII. However, RFX5 can strongly bind to the tripeptidyl peptidase 1 (TPP1) promoter region and then increase its transcriptional activity. We also found that manipulation the expression of RFX5 can significantly affect the expression of TPP1 in HepG2, which suggested that RFX5 can transcriptionally activate TPP1 in HCC. Moreover, TPP1 is overexpressed in HCC tissues and significantly correlated with poor prognosis of HCC patients, suggesting that it may have potential biological implications in HCC.
authors.affiliation: Department of Immunology, and The Key Laboratory of Laboratory Medicine of Jiangsu Province, School of Medicine, Jiangsu University, Zhenjiang, Jiangsu 212013, P.R. China.
authors.forename: Yangjing
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: Peking University People's Hospital, Peking University Hepatology Institute, Beijing Key Laboratory of Hepatitis C and Immunotherapy for Liver Disease, Beijing 100044, P.R. China.
authors.forename: Xingwang
authors.initials: X
authors.lastname: Xie
authors.affiliation: Laboratory of Hepatobiliary and Pancreatic Surgery, The Affiliated Hospital of Guilin Medical University, Guilin, Guangxi 541001, P.R. China.
authors.forename: Weijia
authors.initials: W
authors.lastname: Liao
authors.affiliation: Peking University People's Hospital, Peking University Hepatology Institute, Beijing Key Laboratory of Hepatitis C and Immunotherapy for Liver Disease, Beijing 100044, P.R. China.
authors.forename: Henghui
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Department of Pathology, The First Affiliated Hospital of Zhejiang University, Hangzhou, Zhejiang 310003, P.R. China.
authors.forename: Hui
authors.initials: H
authors.lastname: Cao
authors.affiliation: Peking University People's Hospital, Peking University Hepatology Institute, Beijing Key Laboratory of Hepatitis C and Immunotherapy for Liver Disease, Beijing 100044, P.R. China.
authors.forename: Ran
authors.initials: R
authors.lastname: Fei
authors.affiliation: Peking University People's Hospital, Peking University Hepatology Institute, Beijing Key Laboratory of Hepatitis C and Immunotherapy for Liver Disease, Beijing 100044, P.R. China.
authors.forename: Xueyan
authors.initials: X
authors.lastname: Wang
authors.affiliation: Peking University People's Hospital, Peking University Hepatology Institute, Beijing Key Laboratory of Hepatitis C and Immunotherapy for Liver Disease, Beijing 100044, P.R. China.
authors.forename: Lai
authors.initials: L
authors.lastname: Wei
authors.affiliation: Department of Immunology, and The Key Laboratory of Laboratory Medicine of Jiangsu Province, School of Medicine, Jiangsu University, Zhenjiang, Jiangsu 212013, P.R. China.
authors.forename: Qixiang
authors.initials: Q
authors.lastname: Shao
authors.affiliation: Peking University People's Hospital, Peking University Hepatology Institute, Beijing Key Laboratory of Hepatitis C and Immunotherapy for Liver Disease, Beijing 100044, P.R. China.
authors.forename: Hongsong
authors.initials: H
authors.lastname: Chen
mesh_terms: D000626:Aminopeptidases / Q000235:genetics* / Q000378:metabolism; D006528:Carcinoma, Hepatocellular / Q000235:genetics* / Q000401:mortality; D004152:Dipeptidyl-Peptidases and Tripeptidyl-Peptidases / Q000235:genetics* / Q000378:metabolism; D015972:Gene Expression Regulation, Neoplastic; D005802:Genes, MHC Class II; D056945:Hep G2 Cells; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics* / Q000401:mortality; D011401:Promoter Regions, Genetic; D000071701:Regulatory Factor X Transcription Factors / Q000235:genetics / Q000378:metabolism*; D057057:Serine Proteases / Q000235:genetics* / Q000378:metabolism; D016019:Survival Analysis; D015533:Transcriptional Activation; D000091346:Tripeptidyl-Peptidase 1
chemical_list: C093524:RFX5 protein, human; D000071701:Regulatory Factor X Transcription Factors; D000091346:Tripeptidyl-Peptidase 1; D057057:Serine Proteases; D000626:Aminopeptidases; D004152:Dipeptidyl-Peptidases and Tripeptidyl-Peptidases; C000717267:TPP1 protein, human