title: A chemical proteomic probe for detecting native carrier protein motifs in nonribosomal peptide synthetases.
abstract: Derivatization of a 5'-(vinylsulfonylaminodeoxy)adenosine scaffold with a clickable functionality provided an activity-based probe that was used to label native carrier protein (CP) motifs in nonribosomal peptide synthetases (NRPSs). When coupled with a fluorescent tag, this probe selectively targeted phosphopantetheinylated CPs (holo-form) from recombinant NRPS enzyme systems and in whole proteomes.
authors.affiliation: Department of System Chemotherapy and Molecular Sciences, Division of Bioinformatics and Chemical Genomics, Graduate School of Pharmaceutical Sciences, Kyoto University, Sakyo, Kyoto 606-8501, Japan. fishika@pharm.kyoto-u.ac.jp scseigyo-hisyo@pharm.kyoto-u.ac.jp.
authors.forename: Shota
authors.initials: S
authors.lastname: Kasai
authors.affiliation: Department of System Chemotherapy and Molecular Sciences, Division of Bioinformatics and Chemical Genomics, Graduate School of Pharmaceutical Sciences, Kyoto University, Sakyo, Kyoto 606-8501, Japan. fishika@pharm.kyoto-u.ac.jp scseigyo-hisyo@pharm.kyoto-u.ac.jp.
authors.forename: Fumihiro
authors.initials: F
authors.lastname: Ishikawa
authors.affiliation: Biomolecular Characterization Unit, RIKEN Center for Sustainable Resource Science, 2-1 Hirokawa, Wako, Saitama 351-0198, Japan.
authors.forename: Takehiro
authors.initials: T
authors.lastname: Suzuki
authors.affiliation: Biomolecular Characterization Unit, RIKEN Center for Sustainable Resource Science, 2-1 Hirokawa, Wako, Saitama 351-0198, Japan.
authors.forename: Naoshi
authors.initials: N
authors.lastname: Dohmae
authors.affiliation: Department of System Chemotherapy and Molecular Sciences, Division of Bioinformatics and Chemical Genomics, Graduate School of Pharmaceutical Sciences, Kyoto University, Sakyo, Kyoto 606-8501, Japan. fishika@pharm.kyoto-u.ac.jp scseigyo-hisyo@pharm.kyoto-u.ac.jp.
authors.forename: Hideaki
authors.initials: H
authors.lastname: Kakeya
mesh_terms: D000241:Adenosine / Q000031:analogs & derivatives / Q000737:chemistry*; D020816:Amino Acid Motifs; D002352:Carrier Proteins / Q000737:chemistry* / Q000378:metabolism; D015335:Molecular Probes / Q000737:chemistry*; D015394:Molecular Structure; D010453:Peptide Synthases / Q000737:chemistry* / Q000378:metabolism; D040901:Proteomics
chemical_list: D002352:Carrier Proteins; D015335:Molecular Probes; D010453:Peptide Synthases; C104385:non-ribosomal peptide synthase; D000241:Adenosine