title: Urinary Concentrations of Steroids in Bulls under Anabolic Treatment by Revalor-XS® Implant.
abstract: Despite the European ban of using anabolics in food-producing animals, growth promoters might still be illegally used in the European Union. To control the food chain and guarantee consumers' health, there is a need of highly sensitive analytical methods for the identification of marker residues of such treatments. In the present study, a group of bulls (n= 16) received trenbolone acetate (200 mg) and estradiol (40 mg) by a commercial ear implant during a time range of 71 days, and a second group (n= 16) was kept for control. The aim of the research was to measure the residual urinary concentrations of the administered drugs (β-trenbolone andβ-estradiol), their main metabolites (α-trenbolone andα-estradiol), and possible alterations of the urinary profile of other endogenous hormones metabolically related. The analytical method was based on liquid chromatography-tandem mass spectrometry. Results showed average urinary concentrations ofα-trenbolone andα-estradiol during treatment in the range of (0.81 ÷ 2.1) ng mL-1and (0.96 ÷ 4.4) ng mL-1, respectively, whereasβ-trenbolone andβ-estradiol exhibit urinary concentrations lower than 0.22 ng mL-1in both cases. Data obtained from the urinary profiles of endogenous steroids indicate that they could be useful to indirectly detect the ongoing treatment.
authors.affiliation: Department of Chemistry, Istituto Zooprofilattico Sperimentale delle Venezie, Viale dell'Università 10, 35020 Legnaro, Italy.
authors.forename: Giancarlo
authors.identifier: 0000-0002-7679-6549
authors.initials: G
authors.lastname: Biancotto
authors.affiliation: Department of Chemistry, Istituto Zooprofilattico Sperimentale delle Venezie, Viale dell'Università 10, 35020 Legnaro, Italy.
authors.forename: Roberto
authors.initials: R
authors.lastname: Stella
authors.affiliation: Department of Public Health and Risk Analysis, Istituto Zooprofilattico Sperimentale delle Venezie, Viale dell'Università 10, 35020 Legnaro, Italy.
authors.forename: Federica
authors.initials: F
authors.lastname: Barrucci
authors.affiliation: Department of Chemistry, Istituto Zooprofilattico Sperimentale delle Venezie, Viale dell'Università 10, 35020 Legnaro, Italy.
authors.forename: Francesca
authors.initials: F
authors.lastname: Lega
authors.affiliation: Department of Chemistry, Istituto Zooprofilattico Sperimentale delle Venezie, Viale dell'Università 10, 35020 Legnaro, Italy.
authors.forename: Roberto
authors.initials: R
authors.lastname: Angeletti