title: Prerequisites for Control of Contamination in Fungal Diagnosis.
abstract: Nucleic acid amplification methods facilitate rapid and sensitive detection of clinically relevant fungal pathogens, and can be employed using a variety of patient specimens. However, contamination from various exogenous sources constitutes a serious threat to the validity of amplification-based fungal assays. In this chapter, common origins of fungal contaminants that compromise molecular fungal testing are described, and measures for preventing contamination are proposed. Detailed guidelines for sample handling, reagent selection, contamination screening, and decontamination procedures are provided.
authors.affiliation: Children's Cancer Research Institute (CCRI), St. Anna Kinderkrebsforschung, Vienna, Austria.|LabDia Labordiagnostik GmbH, Vienna, Austria.
authors.forename: Stefan
authors.initials: S
authors.lastname: Czurda
authors.affiliation: Children's Cancer Research Institute (CCRI), St. Anna Kinderkrebsforschung, Vienna, Austria. thomas.lion@ccri.at.|LabDia Labordiagnostik GmbH, Vienna, Austria. thomas.lion@ccri.at.|Department of Pediatrics, Medical University of Vienna, Vienna, Austria. thomas.lion@ccri.at.
authors.forename: Thomas
authors.initials: T
authors.lastname: Lion
mesh_terms: D059018:DNA Contamination; D004271:DNA, Fungal / Q000235:genetics / Q000302:isolation & purification*; D003666:Decontamination; D003851:Deoxyribonucleases / Q000737:chemistry; D004783:Environmental Microbiology; D006801:Humans; D000072742:Invasive Fungal Infections / Q000175:diagnosis* / Q000382:microbiology; D025202:Molecular Diagnostic Techniques
chemical_list: D004271:DNA, Fungal; D003851:Deoxyribonucleases
keywords: Contamination prevention; DNase-mediated decontamination; Invasive fungal infection; Molecular fungal diagnostics; PCR reagent contamination