title: Spatiotemporal fluorescence imaging of newly synthesized proteins in normal and cancerous cells with anticarcinogen modulation.
abstract: Some anticarcinogen could treat cancers through regulating the synthesis and degradation of proteins. Therefore, the rules of newly synthesized proteins would be used to evaluate the effect of anticarcinogen. In this study, we coupled noncanonical amino acid tagging with click reaction to label the newly synthesized proteins in normal cells and cancerous cells. We studied the influence of six different drugs on the protein synthesis. The results showed the differences of protein synthesis rate and spatial distribution in normal cells and cancerous cells, as well as their different responding to anticarcinogen stimulation. This approach could help us to understand the growth of proteins and distinguish cancerous cells from normal cells, which would benefit the diagnosis and monitoring of cancer, as well as evaluating the curative effect of drugs. Based on this strategy, more significant biological process about newly synthesized biomolecules would be studied in-depth.
authors.affiliation: Beijing Key Laboratory of Microanalytical Methods and Instrumentation, Department of Chemistry, Tsinghua University, Beijing 100084, China.
authors.forename: Jinyu
authors.initials: J
authors.lastname: Wang
authors.affiliation: School of Chemistry and Chemical Engineering, State Key Laboratory of Analytical Chemistry for Life Science and Collaborative Innovation Center of Chemistry for Life Science, Nanjing University, Nanjing 210093, China.
authors.forename: Linfeng
authors.initials: L
authors.lastname: Sheng
authors.affiliation: Beijing Key Laboratory of Microanalytical Methods and Instrumentation, Department of Chemistry, Tsinghua University, Beijing 100084, China.
authors.forename: Hansen
authors.initials: H
authors.lastname: Zhao
authors.affiliation: Beijing Key Laboratory of Microanalytical Methods and Instrumentation, Department of Chemistry, Tsinghua University, Beijing 100084, China.
authors.forename: Xinrong
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Beijing Key Laboratory of Microanalytical Methods and Instrumentation, Department of Chemistry, Tsinghua University, Beijing 100084, China. Electronic address: sczhang@mail.tsinghua.edu.cn.
authors.forename: Sichun
authors.initials: S
authors.lastname: Zhang
mesh_terms: D000072283:A549 Cells; D000409:Alanine / Q000031:analogs & derivatives / Q000737:chemistry; D000596:Amino Acids / Q000096:biosynthesis* / Q000737:chemistry; D016588:Anticarcinogenic Agents / Q000494:pharmacology*; D002460:Cell Line; D045744:Cell Line, Tumor; D057930:Click Chemistry; D005453:Fluorescence; D006801:Humans; D061986:MCF-7 Cells; D018613:Microscopy, Confocal; D008956:Models, Chemical; D014176:Protein Biosynthesis / Q000187:drug effects*; D011506:Proteins / Q000737:chemistry / Q000378:metabolism*
chemical_list: D000596:Amino Acids; D016588:Anticarcinogenic Agents; D011506:Proteins; C506098:azidohomoalanine; D000409:Alanine
keywords: Anticarcinogen; Click chemistry; Newly synthesized proteins; Normal and cancerous cells