title: Investigation of thiolysis of NBD amines for the development of H2S probes and evaluating the stability of NBD dyes.
abstract: In order to evaluate the thiolysis of NBD (7-nitro-1,2,3-benzoxadiazole) amines for development of H2S probes, herein we investigated the reactivity and selectivity of a series of NBD amines for the first time. The piperazinyl- and piperidyl-based NBD probes could react efficiently with micromolar H2S in buffer (pH 7.4), while such NBD(S) (nitrobenzothiadiazole) derivatives showed much slow thiolysis even in the presence of millimolar H2S. Low reactivity was also observed for thiolysis of these ethylamino-, ethanolamino- and anilino-based NBD probes. Therefore, almost all NBD amines used in bioimaging should be stable, in consideration of the presence of only micromolar endogenous H2S in vivo. Moreover, the piperazinyl-NBD derivatives could be efficient in the development of fluorescent H2S probes and for directly visualizing H2S by paper-based detection.
authors.affiliation: Tianjin Key Laboratory of Water Resources and Environment, Tianjin Normal University, Tianjin 300387, China.
authors.forename: Fanbo
authors.initials: F
authors.lastname: Song
authors.affiliation: State Key Laboratory of Organic-Inorganic Composites and College of Information Science and Technology, Beijing University of Chemical Technology (BUCT), 15 Beisanhuan East Road, Chaoyang District, Beijing 100029, China. xbqiu@mail.buct.edu.cn yilong@mail.buct.edu.cn.
authors.forename: Zhifei
authors.initials: Z
authors.lastname: Li
authors.affiliation: State Key Laboratory of Organic-Inorganic Composites and College of Information Science and Technology, Beijing University of Chemical Technology (BUCT), 15 Beisanhuan East Road, Chaoyang District, Beijing 100029, China. xbqiu@mail.buct.edu.cn yilong@mail.buct.edu.cn.
authors.forename: Jiayuan
authors.initials: J
authors.lastname: Li
authors.affiliation: State Key Laboratory of Elemento-Organic Chemistry and Department of Chemical Biology, National Engineering Research Center of Pesticide (Tianjin), Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), Nankai University, Tianjin 300071, China.
authors.forename: Shuai
authors.initials: S
authors.lastname: Wu
authors.affiliation: State Key Laboratory of Organic-Inorganic Composites and College of Information Science and Technology, Beijing University of Chemical Technology (BUCT), 15 Beisanhuan East Road, Chaoyang District, Beijing 100029, China. xbqiu@mail.buct.edu.cn yilong@mail.buct.edu.cn.
authors.forename: Xianbo
authors.initials: X
authors.lastname: Qiu
authors.affiliation: State Key Laboratory of Elemento-Organic Chemistry and Department of Chemical Biology, National Engineering Research Center of Pesticide (Tianjin), Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), Nankai University, Tianjin 300071, China.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Xi
authors.affiliation: State Key Laboratory of Organic-Inorganic Composites and College of Information Science and Technology, Beijing University of Chemical Technology (BUCT), 15 Beisanhuan East Road, Chaoyang District, Beijing 100029, China. xbqiu@mail.buct.edu.cn yilong@mail.buct.edu.cn.
authors.forename: Long
authors.initials: L
authors.lastname: Yi