title: Dibenzo-α-pyrones: a new class of larvicidal metabolites against Aedes aegypti from the endophytic fungus Hyalodendriella sp. Ponipodef12.
abstract: BACKGROUND
  In our search for new agrochemicals from endophytic fungi, the crude extract of the endophytic Hyalodendriella sp. Ponipodef12 associated with the hybrid 'Neva' of Populus deltoides Marsh × P. nigra L. was found to possess larvicidal activity against Aedes aegypti.
  
  
  RESULTS
  Fractionation of the extract has led to the isolation of 11 dibenzo-α-pyrones (1-11), including three new congeners: hyalodendriols A-C (1-3). The structures of the new compounds were elucidated by comprehensive spectroscopic analyses, including the modified Mosher's method for the assignment of the absolute configuration. Compounds 2-7 showed potent larvicidal activities against the fourth-instar larvae of A. aegypti with IC50values ranging from 7.21 to 120.81 µg mL-1. Among them, penicilliumolide D (6) displayed the strongest activity (IC50= 7.21 µg mL-1). A structure-larvicidal activity relationship was discussed. The possible mode of action of these compounds was assessed for their acetylcholinesterase inhibitory activities. In addition, hyalodendriol C (3) displayed antibacterial activity against Bacillus subtilis and Xanthomonas vesicatoria, and exhibited strong inhibition against the spore germination of Magnaporthe oryzae.
  
  
  CONCLUSIONS
  Our study revealed dibenzo-α-pyrones to be a new class of larvicidal metabolites against A. aegypti. © 2016 Society of Chemical Industry.
authors.affiliation: Key Laboratory of Plant Pathology, Ministry of Agriculture/Department of Plant Pathology, College of Plant Protection, China Agricultural University, Beijing, China.
authors.forename: Ziling
authors.initials: Z
authors.lastname: Mao
authors.affiliation: Key Laboratory of Plant Pathology, Ministry of Agriculture/Department of Plant Pathology, College of Plant Protection, China Agricultural University, Beijing, China.
authors.forename: Daowan
authors.initials: D
authors.lastname: Lai
authors.affiliation: Key Laboratory of Plant Pathology, Ministry of Agriculture/Department of Plant Pathology, College of Plant Protection, China Agricultural University, Beijing, China.
authors.forename: Xunda
authors.initials: X
authors.lastname: Liu
authors.affiliation: Key Laboratory of Plant Pathology, Ministry of Agriculture/Department of Plant Pathology, College of Plant Protection, China Agricultural University, Beijing, China.
authors.forename: Xiaoxiang
authors.initials: X
authors.lastname: Fu
authors.affiliation: Key Laboratory of Plant Pathology, Ministry of Agriculture/Department of Plant Pathology, College of Plant Protection, China Agricultural University, Beijing, China.
authors.forename: Jiajia
authors.initials: J
authors.lastname: Meng
authors.affiliation: Key Laboratory of Plant Pathology, Ministry of Agriculture/Department of Plant Pathology, College of Plant Protection, China Agricultural University, Beijing, China.
authors.forename: Ali
authors.initials: A
authors.lastname: Wang
authors.affiliation: Key Laboratory of Plant Pathology, Ministry of Agriculture/Department of Plant Pathology, College of Plant Protection, China Agricultural University, Beijing, China.
authors.forename: Xiaohan
authors.initials: X
authors.lastname: Wang
authors.affiliation: Key Laboratory of Plant Pathology, Ministry of Agriculture/Department of Plant Pathology, College of Plant Protection, China Agricultural University, Beijing, China.
authors.forename: Weibo
authors.initials: W
authors.lastname: Sun
authors.affiliation: Department of Entomology, College of Plant Protection, China Agricultural University, Beijing, China.
authors.forename: Zhi Long
authors.initials: ZL
authors.lastname: Liu
authors.affiliation: Key Laboratory of Plant Pathology, Ministry of Agriculture/Department of Plant Pathology, College of Plant Protection, China Agricultural University, Beijing, China.
authors.forename: Ligang
authors.initials: L
authors.lastname: Zhou
authors.affiliation: Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences/Key Laboratory of Agro-products Processing, Ministry of Agriculture, Beijing, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Liu
mesh_terms: D000330:Aedes / Q000187:drug effects*; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001203:Ascomycota / Q000737:chemistry* / Q000378:metabolism; D001412:Bacillus subtilis / Q000187:drug effects; D001688:Biological Products / Q000737:chemistry* / Q000494:pharmacology*; D005659:Fungicides, Industrial / Q000494:pharmacology; D007306:Insecticides; D007814:Larva / Q000187:drug effects; D020082:Magnaporthe / Q000187:drug effects; D011753:Pyrones / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D013329:Structure-Activity Relationship; D044184:Xanthomonas vesicatoria / Q000187:drug effects
chemical_list: D000900:Anti-Bacterial Agents; D001688:Biological Products; D005659:Fungicides, Industrial; D007306:Insecticides; D011753:Pyrones
keywords: Aedes aegypti; Hyalodendriella; antibacterial; antifungal; dibenzo-α-pyrone; larvicidal activity; mosquito