title: Is it feasible to use granulocyte-colony stimulating factor alone to mobilize progenitor cells in multiple myeloma patients induced with a cyclophosphamide, thalidomide and dexamethasone regimen?
abstract: BACKGROUND
  Cyclophosphamide plus thalidomide as induction for multiple myeloma patients eligible for autologous stem cell transplantation may be a limiting factor for cell mobilization. The minimum acceptable mobilized peripheral blood stem cell count to prevent deleterious effects during transplantation is 2.0×106CD34+cells/kg. Combining other treatments to granulocyte-colony stimulating factor, such as cyclophosphamide, could overcome the mobilization limitation. The objective of this study was to assess the number of CD34+cells mobilized using granulocyte-colony stimulating factor with and without cyclophosphamide after induction with cyclophosphamide, thalidomide and dexamethasone.
  
  
  METHODS
  A retrospective study was performed of a cohort of multiple myeloma patients submitted to autologous stem cell transplantations at two Brazilian centers between May 2009 and July 2013. The oral cyclophosphamide and thalidomide induction doses used were 1500mg/month and 100-200mg/day, respectively. Mobilization doses were 10-15mcg/kg granulocyte-colony stimulating factor with 2-4g/m2cyclophosphamide, or 15-20mcg/kg granulocyte-colony stimulating factor alone for 5 days. Collection of >2.0×106CD34+cells/kg was considered sufficient.
  
  
  RESULTS
  Eighty-eight patients were analyzed; only 18 received cyclophosphamide. The median age was 58 years old (range: 51-62) for the granulocyte-colony stimulating factor group and 56.5 years old (range: 54-60) for granulocyte-colony stimulating factor plus cyclophosphamide group. Fifty-two patients were male. Eighty cases (90.9%) were Durie-Salmon Staging System III-A/B and 38 (44.7%) and 20 cases (23.5%) were International Staging System 2 and 3, respectively. The group that received cyclophosphamide collected a higher median number of progenitor cells [3.8 (range: 3.1-4.4) vs. 3.2 (range: 2.3-3.8)] (p-value=0.008). No correlation was observed between better responses or number of induction cycles and the number of cells collected.
  
  
  CONCLUSIONS
  The number of cells mobilized with granulocyte-colony stimulating factor plus cyclophosphamide was higher. However, in both groups, the median number of CD34+cells was sufficient to perform a single autologous stem cell transplantation; no deleterious effects were reported during harvesting.
authors.affiliation: Faculdade de Ciências Médicas da Santa Casa de São Paulo (FCMSCSP), São Paulo, SP, Brazil. Electronic address: edvancrusoe@gmail.com.
authors.forename: Edvan de Queiroz
authors.initials: EQ
authors.lastname: Crusoe
authors.affiliation: Faculdade de Ciências Médicas da Santa Casa de São Paulo (FCMSCSP), São Paulo, SP, Brazil.
authors.forename: Fabiana
authors.initials: F
authors.lastname: Higashi
authors.affiliation: Universidade de São Paulo (USP), Faculdade de Medicina, Hospital das Clínicas, Instituto do Câncer do Estado de São Paulo Octavio Frias de Oliveira (ICESP), São Paulo, SP, Brazil.
authors.forename: Gracia Aparecida
authors.initials: GA
authors.lastname: Martinez
authors.affiliation: Faculdade de Ciências Médicas da Santa Casa de São Paulo (FCMSCSP), São Paulo, SP, Brazil.
authors.forename: José Carlos
authors.initials: JC
authors.lastname: Barros
authors.affiliation: Universidade de São Paulo (USP), Faculdade de Medicina, Hospital das Clínicas, Instituto do Câncer do Estado de São Paulo Octavio Frias de Oliveira (ICESP), São Paulo, SP, Brazil.
authors.forename: Marcelo
authors.initials: M
authors.lastname: Bellesso
authors.affiliation: Faculdade de Ciências Médicas da Santa Casa de São Paulo (FCMSCSP), São Paulo, SP, Brazil.
authors.forename: Marina
authors.initials: M
authors.lastname: Rossato
authors.affiliation: Faculdade de Ciências Médicas da Santa Casa de São Paulo (FCMSCSP), São Paulo, SP, Brazil.
authors.forename: Ana Cinira F
authors.initials: AC
authors.lastname: Marret
authors.affiliation: Faculdade de Ciências Médicas da Santa Casa de São Paulo (FCMSCSP), São Paulo, SP, Brazil.
authors.forename: Carlos Sérgio
authors.initials: CS
authors.lastname: Chiattone
authors.affiliation: Faculdade de Ciências Médicas da Santa Casa de São Paulo (FCMSCSP), São Paulo, SP, Brazil.
authors.forename: Vania Tietsch de Moraes
authors.initials: VT
authors.lastname: Hungria
keywords: Bone marrow transplantation; Cyclophosphamide; Granulocyte-colony stimulating factor; Multiple myeloma