title: Piper sarmentosum Effects on 11β-Hydroxysteroid Dehydrogenase Type 1 Enzyme in Serum and Bone in Rat Model of Glucocorticoid-Induced Osteoporosis.
abstract: Glucocorticoid-induced osteoporosis is one of the common causes of secondary osteoporosis.Piper sarmentosum(Ps) extract possesses antioxidant and anti-inflammatory activities. In this study, we determined the correlation between the effects ofPsleaf water extract with the regulation of 11β-hydroxysteroid dehydrogenase (HSD) type 1 enzyme activity in serum and bone of glucocorticoid-induced osteoporotic rats. Twenty-four Sprague-Dawley rats were grouped into following: G1: sham-operated group administered with intramuscular vehicle olive oil and vehicle normal saline orally; G2: adrenalectomized (adrx) control group given intramuscular dexamethasone (120 μg/kg/day) and vehicle normal saline orally; G3: adrx group given intramuscular dexamethasone (120 μg/kg/day) and water extract ofPiper sarmentosum(125 mg/kg/day) orally. After two months, the femur and serum were taken for ELISA analysis. Results showed thatPsleaf water extract significantly reduced the femur corticosterone concentration (p< 0.05). This suggests thatPsleaf water extract was able to prevent bone loss due to long-term glucocorticoid therapy by acting locally on the bone cells by increasing the dehydrogenase action of 11β-HSD type 1. Thus,Psmay have the potential to be used as an alternative medicine against osteoporosis and osteoporotic fracture in patients on long-term glucocorticoid treatment.
authors.affiliation: Department of Anatomy, Faculty of Medicines, Universiti Kebangsaan Malaysia Medical Centre (UKMMC), Jalan Yaacob Latif, Bandar Tun Razak, 56000 Cheras, Kuala Lumpur, Malaysia. fadziyah@yahoo.com.my.|Department of Human Anatomy, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia. fadziyah@yahoo.com.my.
authors.forename: Siti Fadziyah
authors.initials: SF
authors.lastname: Mohamad Asri
authors.affiliation: Department of Anatomy, Faculty of Medicines, Universiti Kebangsaan Malaysia Medical Centre (UKMMC), Jalan Yaacob Latif, Bandar Tun Razak, 56000 Cheras, Kuala Lumpur, Malaysia. elvysuhana@yahoo.com.my.
authors.forename: Elvy Suhana
authors.initials: ES
authors.lastname: Mohd Ramli
authors.affiliation: Department of Pharmacology, Faculty of Medicines, Universiti Kebangsaan Malaysia Medical Centre (UKMMC), Jalan Yaacob Latif, Bandar Tun Razak, 56000 Cheras, Kuala Lumpur, Malaysia. imasoel@ppukm.ukm.edu.my.
authors.forename: Ima Nirwana
authors.initials: IN
authors.lastname: Soelaiman
authors.affiliation: Department of Anatomy, Faculty of Medicine, Universiti Malaya Medical Centre (UMMC), 59100 Lembah Pantai, Kuala Lumpur, Malaysia. pae2300@yahoo.com.
authors.forename: Muhamad Alfakry
authors.initials: MA
authors.lastname: Mat Noh
authors.affiliation: Anatomy Unit, Basic Medical Science Cluster, Faculty of Medicine, Universiti Teknologi MARA (UiTM), Sungai Buloh Campus, Jalan Hospital, 47000 Sungai Buloh, Selangor, Malaysia. dahar_219@yahoo.com.
authors.forename: Abdul Hamid
authors.initials: AH
authors.lastname: Abdul Rashid
authors.affiliation: Department of Anatomy, Faculty of Medicines, Universiti Kebangsaan Malaysia Medical Centre (UKMMC), Jalan Yaacob Latif, Bandar Tun Razak, 56000 Cheras, Kuala Lumpur, Malaysia. farihah@ppukm.ukm.edu.my.
authors.forename: Farihah
authors.initials: F
authors.lastname: Suhaimi
mesh_terms: D043205:11-beta-Hydroxysteroid Dehydrogenase Type 1 / Q000097:blood / Q000378:metabolism*; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000737:chemistry / Q000494:pharmacology; D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology; D015415:Biomarkers; D001835:Body Weight / Q000187:drug effects; D001842:Bone and Bones / Q000187:drug effects* / Q000378:metabolism*; D003345:Corticosterone / Q000097:blood / Q000378:metabolism; D004195:Disease Models, Animal; D005938:Glucocorticoids / Q000009:adverse effects*; D010024:Osteoporosis / Q000097:blood / Q000188:drug therapy / Q000209:etiology* / Q000378:metabolism*; D031701:Piper / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D051381:Rats
chemical_list: D000893:Anti-Inflammatory Agents; D000975:Antioxidants; D015415:Biomarkers; D005938:Glucocorticoids; D010936:Plant Extracts; D043205:11-beta-Hydroxysteroid Dehydrogenase Type 1; D003345:Corticosterone
keywords: 11β-hydroxysteroid dehydrogenase; Piper sarmentosum; glucocorticoid; osteoporosis