title: Comparative proteomic analyses of the parietal lobe from rhesus monkeys fed a high-fat/sugar diet with and without resveratrol supplementation, relative to a healthy diet: Insights into the roles of unhealthy diets and resveratrol on function.
abstract: A diet consisting of a high intake of saturated fat and refined sugars is characteristic of a Western-diet and has been shown to have a substantial negative effect on human health. Expression proteomics were used to investigate changes to the parietal lobe proteome of rhesus monkeys consuming either a high fat and sugar (HFS) diet, a HFS diet supplemented with resveratrol (HFS+RSV), or a healthy control diet for 2 years. Here we discuss the modifications in the levels of 12 specific proteins involved in various cellular systems including metabolism, neurotransmission, structural integrity, and general cellular signaling following a nutritional intervention. Our results contribute to a better understanding of the mechanisms by which resveratrol functions through the up- or down-regulation of proteins in different cellular sub-systems to affect the overall health of the brain.
authors.affiliation: Department of Chemistry, University of Kentucky, Lexington, KY 40536, USA; Sanders-Brown Center on Aging, University of Kentucky, Lexington, KY 40536, USA.
authors.forename: Aaron M
authors.initials: AM
authors.lastname: Swomley
authors.affiliation: Department of Chemistry, University of Kentucky, Lexington, KY 40536, USA; Sanders-Brown Center on Aging, University of Kentucky, Lexington, KY 40536, USA.
authors.forename: Judy C
authors.initials: JC
authors.lastname: Triplett
authors.affiliation: Department of Chemistry, University of Kentucky, Lexington, KY 40536, USA.
authors.forename: Jeriel T
authors.initials: JT
authors.lastname: Keeney
authors.affiliation: Department of Chemistry, University of Kentucky, Lexington, KY 40536, USA.
authors.forename: Govind
authors.initials: G
authors.lastname: Warrier
authors.affiliation: Sanders-Brown Center on Aging, University of Kentucky, Lexington, KY 40536, USA; Molecular and Biomedical Pharmacology and Nutrition, University of Kentucky, Lexington, KY 40536, USA.
authors.forename: Kevin J
authors.initials: KJ
authors.lastname: Pearson
authors.affiliation: Translational Gerontology Branch, National Institute on Aging, NIH, Baltimore, MD 21224, USA.
authors.forename: Julie A
authors.initials: JA
authors.lastname: Mattison
authors.affiliation: Translational Gerontology Branch, National Institute on Aging, NIH, Baltimore, MD 21224, USA.
authors.forename: Rafael
authors.initials: R
authors.lastname: de Cabo
authors.affiliation: Proteomics Center, University of Louisville, Louisville, KY 40202, USA.
authors.forename: Jian
authors.initials: J
authors.lastname: Cai
authors.affiliation: Proteomics Center, University of Louisville, Louisville, KY 40202, USA.
authors.forename: Jon B
authors.initials: JB
authors.lastname: Klein
authors.affiliation: Department of Chemistry, University of Kentucky, Lexington, KY 40536, USA; Sanders-Brown Center on Aging, University of Kentucky, Lexington, KY 40536, USA. Electronic address: dabcns@uky.edu.
authors.forename: D Allan
authors.initials: DA
authors.lastname: Butterfield
mesh_terms: D063309:2',3'-Cyclic Nucleotide 3'-Phosphodiesterase / Q000235:genetics / Q000378:metabolism; D000818:Animals; D001921:Brain / Q000187:drug effects / Q000378:metabolism; D000072001:Diet, Healthy; D059305:Diet, High-Fat / Q000009:adverse effects*; D000073417:Dietary Sugars / Q000008:administration & dosage / Q000009:adverse effects*; D019587:Dietary Supplements; D004195:Disease Models, Animal; D005974:Glutamate-Ammonia Ligase / Q000235:genetics / Q000378:metabolism; D008253:Macaca mulatta; D008297:Male; D010296:Parietal Lobe / Q000378:metabolism*; D020543:Proteome / Q000378:metabolism*; D015203:Reproducibility of Results; D000077185:Resveratrol; D015398:Signal Transduction; D013267:Stilbenes / Q000494:pharmacology*
chemical_list: D000073417:Dietary Sugars; D020543:Proteome; D013267:Stilbenes; D063309:2',3'-Cyclic Nucleotide 3'-Phosphodiesterase; D005974:Glutamate-Ammonia Ligase; D000077185:Resveratrol
keywords: Brain; Proteomics; Resveratrol; Rhesus monkey; Western diet