title: Chenopodium album Linn. leaves prevent ethylene glycol-induced urolithiasis in rats.
abstract: BACKGROUND
  The leaves of Chenopodium album Linn. are traditionally used for correction of kidney diseases and urinary stones. The present work investigated the effect of methanolic and aqueous extracts of leaves of Chenopodium album on experimentally-induced urolithiasis in rats to substantiate its traditional use as antilithiatic agent.
  
  
  METHODS
  The leaf extract was standardized by HPLC. Urolithiasis was induced in rats by administration of 0.75% v/v of ethylene glycol (EG) in distilled water and in addition, vehicle or methanol (CAME) or aqueous (CAAE) extract of the leaves of Chenopodium album each in the dose 100, 200 and 400mg/kg or Cystone (750mg/kg) were administered daily orally for 28 days. Urolithiasis was assessed by estimating the calcium, phosphorus, urea, uric acid, and creatinine in both urine and plasma. The volume, pH and oxalate levels were also estimated in urine. The renal oxalate content was estimated in kidney while calcium oxalate deposits were observed histologically.
  
  
  RESULTS
  The treatment with CAME or CAAE for 28 days significantly attenuated the EG-induced elevations in the urine and plasma levels of calcium, phosphorus, urea, uric acid and creatinine along with decrease in urine volume, pH and oxalates. The treatments also decreased renal tissue oxalate and deposition of oxalate crystals in kidney due to EG treatment. The effects of CAME and CAAE were comparable to standard antilithiatic agent, cystone. The findings indicate the preventive effect of CAME and CAAE which can be due to inhibitory effect on crystallization and stone dissolution. The effect was attributed to the presence of phytochemicals like flavonoids and saponins.
  
  
  CONCLUSIONS
  In conclusion, Chenopodium album leaves exhibited antilithiatic effect and validates its ethnomedicinal use in urinary disorders and kidney stones.
authors.affiliation: Shri Ram College of Pharmacy, Banmore 474 011, Madhya Pradesh, India.
authors.forename: Indu
authors.initials: I
authors.lastname: Sikarwar
authors.affiliation: National Research Institute for Ayurveda-Siddha Human Resource Development, Aamkho, Gwalior 474 009, Madhya Pradesh, India.
authors.forename: Yadu Nandan
authors.initials: YN
authors.lastname: Dey
authors.affiliation: National Research Institute for Ayurveda-Siddha Human Resource Development, Aamkho, Gwalior 474 009, Madhya Pradesh, India. Electronic address: manish.nriashrd@gmail.com.
authors.forename: Manish M
authors.initials: MM
authors.lastname: Wanjari
authors.affiliation: Shri Ram College of Pharmacy, Banmore 474 011, Madhya Pradesh, India.
authors.forename: Ajay
authors.initials: A
authors.lastname: Sharma
authors.affiliation: Central Council for Research in Ayurvedic Sciences, Ministry of AYUSH, New Delhi 110 058, India.
authors.forename: Sudesh N
authors.initials: SN
authors.lastname: Gaidhani
authors.affiliation: National Research Institute for Ayurveda-Siddha Human Resource Development, Aamkho, Gwalior 474 009, Madhya Pradesh, India.
authors.forename: Ankush D
authors.initials: AD
authors.lastname: Jadhav
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000097:blood / Q000652:urine; D027463:Chenopodium album / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D003460:Crystallization; D004195:Disease Models, Animal; D019855:Ethylene Glycol; D005260:Female; D005419:Flavonoids / Q000302:isolation & purification / Q000494:pharmacology; D007668:Kidney / Q000187:drug effects* / Q000378:metabolism / Q000503:physiopathology; D008297:Male; D000432:Methanol / Q000737:chemistry; D008517:Phytotherapy; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology* / Q000633:toxicity; D018515:Plant Leaves / Q000737:chemistry*; D010946:Plants, Medicinal; D017208:Rats, Wistar; D012503:Saponins / Q000302:isolation & purification / Q000494:pharmacology; D012997:Solvents / Q000737:chemistry; D013997:Time Factors; D014554:Urination / Q000187:drug effects; D052878:Urolithiasis / Q000097:blood / Q000139:chemically induced / Q000517:prevention & control* / Q000652:urine; D064804:Urological Agents / Q000302:isolation & purification / Q000494:pharmacology* / Q000633:toxicity; D014867:Water / Q000737:chemistry
chemical_list: D015415:Biomarkers; D005419:Flavonoids; D010936:Plant Extracts; D012503:Saponins; D012997:Solvents; D064804:Urological Agents; C041060:cystone; D014867:Water; D019855:Ethylene Glycol; D000432:Methanol
keywords: Bathua; Calcium oxalate; Cystone; Ethylene glycol; Urolithiasis