title: Epidemiological Characteristics of Strongyloidiasis in Inhabitants of Indigenous Communities in Borneo Island, Malaysia.
abstract: Epidemiological study on strongyloidiasis in humans is currently lacking in Malaysia. Thus, a cross-sectional study was carried out to determine the prevalence ofStrongyloides stercoralisinfection among the inhabitants of longhouse indigenous communities in Sarawak. A single stool and blood sample were collected from each participant and subjected to microscopy, serological and molecular techniques. Five species of intestinal parasites were identified by stool microscopy. None of the stool samples were positive forS. stercoralis. However, 11% of 236 serum samples were seropositive for strongyloidiasis. Further confirmation using molecular technique on stool samples of the seropositive individuals successfully amplified 5 samples, suggesting current active infections. The prevalence was significantly higher in adult males and tended to increase with age.S. stercoralisshould no longer be neglected in any intestinal parasitic survey. Combination of more than 1 diagnostic technique is necessary to increase the likelihood of estimating the 'true' prevalence ofS. stercoralis.
authors.affiliation: Department of Parasitology, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia.
authors.forename: Romano
authors.initials: R
authors.lastname: Ngui
authors.affiliation: Department of Parasitology, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia.
authors.forename: Noor Amira Abdul
authors.initials: NA
authors.lastname: Halim
authors.affiliation: Department of Parasitology, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia.|International Medical University, No.126, Jalan 19/155B, Bukit Jalil, 57000 Kuala Lumpur, Malaysia.
authors.forename: Yamuna
authors.initials: Y
authors.lastname: Rajoo
authors.affiliation: Department of Parasitology, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia.
authors.forename: Yvonne Al
authors.initials: YA
authors.lastname: Lim
authors.affiliation: International Medical University, No.126, Jalan 19/155B, Bukit Jalil, 57000 Kuala Lumpur, Malaysia.
authors.forename: Stephen
authors.initials: S
authors.lastname: Ambu
authors.affiliation: Hospital Sarikei, Jalan Rentap 96100, Sarikei, Sarawak, Malaysia.
authors.forename: Komalaveni
authors.initials: K
authors.lastname: Rajoo
authors.affiliation: Hospital Sarikei, Jalan Rentap 96100, Sarikei, Sarawak, Malaysia.
authors.forename: Tey Siew
authors.initials: TS
authors.lastname: Chang
authors.affiliation: Hospital Sarikei, Jalan Rentap 96100, Sarikei, Sarawak, Malaysia.
authors.forename: Lu Chan
authors.initials: LC
authors.lastname: Woon
authors.affiliation: Department of Parasitology, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia.
authors.forename: Rohela
authors.initials: R
authors.lastname: Mahmud
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000818:Animals; D001769:Blood / Q000469:parasitology; D001893:Borneo / Q000453:epidemiology; D002648:Child; D002675:Child, Preschool; D003430:Cross-Sectional Studies; D005243:Feces / Q000469:parasitology; D005260:Female; D006801:Humans; D007223:Infant; D008297:Male; D008853:Microscopy; D008875:Middle Aged; D025202:Molecular Diagnostic Techniques; D044382:Population Groups; D015995:Prevalence; D012698:Serologic Tests; D017171:Strongyloides stercoralis / Q000302:isolation & purification*; D013322:Strongyloidiasis / Q000453:epidemiology*; D055815:Young Adult
keywords: ELISA; Malaysia; PCR; Strongyloides stercoralis; soil-transmitted helminth (STH)