title: Endothelium-Dependent Vasorelaxant Effects of Dealcoholized Wine Powder of Wild Grape (Vitis coignetiae) in the Rat Thoracic Aorta.
abstract: The vasorelaxant effects of dealcoholized wild grape (Vitis coignetiae) wine were investigated with isolated rat thoracic aorta. In our present study, we demonstrate that wild grape wine powder (WGWP) induced relaxation of aortic rings preconstricted with norepinephrine in a dose-dependent manner (at concentrations ranging from 0.1 to 1 mg/mL). The vasorelaxant effect of WGWP was dependent on intact endothelia, which was attenuated by incubation with inhibitors of endothelium-derived relaxing factors, such as NG-nitro-L-arginine (nitric oxide synthase inhibitor), methylene blue (guanylate cyclase inhibitor), and indomethacin (cyclooxygenase inhibitor). Moreover, treatment with WGWP and atropine (muscarinic receptor antagonist) or diphenylhydramine (histamine receptor antagonist) significantly inhibited endothelium-dependent vasorelaxation. Our results suggest that WGWP induces relaxation in rat aortic rings in an endothelium-dependent manner. Results further indicate that this effect occurs via nitric oxide-cGMP pathway and prostacyclin-cAMP pathway through a muscarinic receptor and histamine receptor.
authors.affiliation: Division of Functional Food Research, Korea Food Research Institute, Gyeonggi 463-746, Republic of Korea.
authors.forename: Sang Keun
authors.initials: SK
authors.lastname: Ha
authors.affiliation: Division of Functional Food Research, Korea Food Research Institute, Gyeonggi 463-746, Republic of Korea.
authors.forename: Ho-Young
authors.initials: HY
authors.lastname: Park
authors.affiliation: Division of Functional Food Research, Korea Food Research Institute, Gyeonggi 463-746, Republic of Korea.
authors.forename: Mee-Ra
authors.initials: MR
authors.lastname: Ryu
authors.affiliation: Division of Functional Food Research, Korea Food Research Institute, Gyeonggi 463-746, Republic of Korea.
authors.forename: Yoonsook
authors.initials: Y
authors.lastname: Kim
authors.affiliation: Division of Functional Food Research, Korea Food Research Institute, Gyeonggi 463-746, Republic of Korea.
authors.forename: Yongkon
authors.identifier: 0000-0003-1226-0574
authors.initials: Y
authors.lastname: Park