title: Diversification of the kinetic properties of yeast NADP-glutamate-dehydrogenase isozymes proceeds independently of their evolutionary origin.
abstract: In the yeast Saccharomyces cerevisiae, the ScGDH1 and ScGDH3 encoded glutamate dehydrogenases (NADP-GDHs) catalyze the synthesis of glutamate from ammonium and α-ketoglutarate (α-KG). Previous kinetic characterization showed that these enzymes displayed different allosteric properties and respectively high or low rate of α-KG utilization. Accordingly, the coordinated action of ScGdh1 and ScGdh3, regulated balanced α-KG utilization for glutamate biosynthesis under either fermentative or respiratory conditions, safeguarding energy provision. Here, we have addressed the question of whether there is a correlation between the regulation and kinetic properties of the NADP-GDH isozymes present in S. cerevisiae (ScGdh1 and ScGdh3), Kluyveromyces lactis (KlGdh1), and Lachancea kluyveri (LkGdh1) and their evolutionary history. Our results show that the kinetic properties of K. lactis and L. kluyveri single NADP-GDHs are respectively similar to either ScGDH3 or ScGDH1, which arose from the whole genome duplication event of the S. cerevisiae lineage, although, KlGDH1 and LkGDH1 originated from a GDH clade, through an ancient interspecies hybridization event that preceded the divergence between the Saccharomyces clade and the one containing the genera Kluyveromyces, Lachancea, and Eremothecium. Thus, the kinetic properties which determine the NADP-GDHs capacity to utilize α-KG and synthesize glutamate do not correlate with their evolutionary origin.
authors.affiliation: Departamento de Bioquímica y Biología Estructural, Instituto de Fisiología Celular, Universidad Nacional Autónoma de México, Mexico City, México.
authors.forename: Carlos
authors.initials: C
authors.lastname: Campero-Basaldua
authors.affiliation: Laboratorio de Inmunología y Proteómica, Hospital Infantil de México Federico Gómez, Mexico City, México.
authors.forename: Héctor
authors.initials: H
authors.lastname: Quezada
authors.affiliation: División de Biología Molecular, IPICYT, San Luis Potosí, México.
authors.forename: Lina
authors.initials: L
authors.lastname: Riego-Ruíz
authors.affiliation: Departamento de Bioquímica y Biología Estructural, Instituto de Fisiología Celular, Universidad Nacional Autónoma de México, Mexico City, México.
authors.forename: Dariel
authors.initials: D
authors.lastname: Márquez
authors.affiliation: Departamento de Bioquímica y Biología Estructural, Instituto de Fisiología Celular, Universidad Nacional Autónoma de México, Mexico City, México.
authors.forename: Erendira
authors.initials: E
authors.lastname: Rojas
authors.affiliation: Departamento de Bioquímica y Biología Estructural, Instituto de Fisiología Celular, Universidad Nacional Autónoma de México, Mexico City, México.
authors.forename: James
authors.initials: J
authors.lastname: González
authors.affiliation: Departamento de Biomedicina Cardiovascular, Instituto Nacional de Cardiología Ignacio Chávez, Mexico City, México.
authors.forename: Mohammed
authors.initials: M
authors.lastname: El-Hafidi
authors.affiliation: Departamento de Bioquímica y Biología Estructural, Instituto de Fisiología Celular, Universidad Nacional Autónoma de México, Mexico City, México.
authors.forename: Alicia
authors.initials: A
authors.lastname: González
mesh_terms: D000595:Amino Acid Sequence; D005075:Biological Evolution; D019143:Evolution, Molecular; D018086:Glutamate Dehydrogenase (NADP+) / Q000235:genetics* / Q000378:metabolism; D005971:Glutamates / Q000096:biosynthesis; D007656:Ketoglutaric Acids / Q000378:metabolism; D007716:Kluyveromyces / Q000201:enzymology* / Q000235:genetics*; D020033:Protein Isoforms / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000201:enzymology* / Q000235:genetics* / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics* / Q000378:metabolism
chemical_list: D005971:Glutamates; D007656:Ketoglutaric Acids; D020033:Protein Isoforms; D029701:Saccharomyces cerevisiae Proteins; C000609733:GDH1 protein, S cerevisiae; C579079:GDH3 protein, S cerevisiae; D018086:Glutamate Dehydrogenase (NADP+)
keywords: functional diversification; glutamate dehydrogenase; kinetics; paralogous enzymes; phylogeny; yeast gene duplication