title: Enhanced production of fructosyltransferase in Aspergillus oryzae by genome shuffling.
abstract: OBJECTIVE
  To breed Aspergillus oryzae strains with high fructosyltransferase (FTase) activity using intraspecific protoplast fusion via genome-shuffling.
  
  
  RESULTS
  A candidate library was developed using UV/LiCl of the conidia of A. oryzae SBB201. By screening for enzyme activity and cell biomass, two mutants (UV-11 and UV-76) were chosen for protoplast fusion and subsequent genome shuffling. After three rounds of genome recombination, a fusion mutant RIII-7 was obtained. Its FTase activity was 180 U g-1, approximately double that of the original strain, and RIII-7 was genetically stable. In fermentation culture, FTase activity of the genome-shuffled strain reached a maximum of 353 U g-1using substrate-feeding method, and this value was approximately 3.4-times higher than that of the original strain A. oryzae SBB201.
  
  
  CONCLUSIONS
  Intraspecific protoplast fusion of A. oryzae significantly enhanced FTase activity and generated a potentially useful strain for industrial production.
authors.affiliation: School of Bioscience and Bioengineering, Guangzhou Higher Education Mega Centre, South China University of Technology, Panyu District, Guangzhou, 510006, China.
authors.forename: Shenghai
authors.initials: S
authors.lastname: Wang
authors.affiliation: School of Bioscience and Bioengineering, Guangzhou Higher Education Mega Centre, South China University of Technology, Panyu District, Guangzhou, 510006, China.
authors.forename: Mengjie
authors.initials: M
authors.lastname: Duan
authors.affiliation: School of Bioscience and Bioengineering, Guangzhou Higher Education Mega Centre, South China University of Technology, Panyu District, Guangzhou, 510006, China.
authors.forename: Yalan
authors.initials: Y
authors.lastname: Liu
authors.affiliation: School of Bioscience and Bioengineering, Guangzhou Higher Education Mega Centre, South China University of Technology, Panyu District, Guangzhou, 510006, China.
authors.forename: Sen
authors.initials: S
authors.lastname: Fan
authors.affiliation: School of Bioscience and Bioengineering, Guangzhou Higher Education Mega Centre, South China University of Technology, Panyu District, Guangzhou, 510006, China.
authors.forename: Xiaoshan
authors.initials: X
authors.lastname: Lin
authors.affiliation: School of Bioscience and Bioengineering, Guangzhou Higher Education Mega Centre, South China University of Technology, Panyu District, Guangzhou, 510006, China. btyzhang@scut.edu.cn.
authors.forename: Yi
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D001236:Aspergillus oryzae / Q000187:drug effects / Q000201:enzymology*; D032621:DNA Shuffling / Q000379:methods*; D005285:Fermentation / Q000187:drug effects; D016681:Genome, Fungal; D042822:Genomic Instability; D006602:Hexosyltransferases / Q000096:biosynthesis*; D008561:Membrane Fusion / Q000187:drug effects; D016296:Mutagenesis / Q000235:genetics; D009154:Mutation / Q000235:genetics; D011523:Protoplasts / Q000187:drug effects / Q000378:metabolism; D012038:Regeneration / Q000187:drug effects; D013395:Sucrose / Q000494:pharmacology; D001426:Bacterial Proteins
chemical_list: D006602:Hexosyltransferases; D013395:Sucrose; C021451:inulosucrase; D001426:Bacterial Proteins
keywords: Aspergillus oryzae; Fructo-oligosaccharides; Fructosyltransferase; Genome shuffling; Protoplast fusion