title: Glycine increases preimplantation development of mouse oocytes following vitrification at the germinal vesicle stage.
abstract: Ice-free cryopreservation, referred to as vitrification, is receiving increased attention in the human and animal assisted reproduction. However, it introduces the detrimental osmotic stress by adding and removing high contents of cryoprotectants. In this study, we evaluated the effects of normalizing cell volume regulation by adding glycine, an organic osmolyte, during vitrification of mouse germinal vesicle stage oocyte and/or subsequent maturation on its development. The data showed that glycine supplementation in either vitrification/thawing or maturation medium significantly improved the cytoplasmic maturation of MII oocytes manifested by spindle assembly, chromosomal alignment, mitochondrial distribution, euploidy rate, and blastocyst development following fertilization in vitro, compared to the control without glycine treatment. Furthermore, glycine addition during both vitrification/thawing and maturation further enhanced the oocyte quality demonstrated by various markers, including ATP contents and embryo development. Lastly, the effect of anti-apoptosis was also observed when glycine was added during vitrification. Our result suggests that reducing osmotic stress induced by vitrification could improve the development of vitrified mouse oocyte.
authors.affiliation: Institute of Special Animal and Plant Sciences, Chinese Academy of Agricultural Sciences, No.4899 Juye Street, Jingyue District, Changchun 130112, China.|State Key Laboratory for Molecular Biology of Special Economic Animal and Plant Science, Chinese Academy of Agricultural Sciences, Changchun 130112, P. R. China.
authors.forename: Xin-Yan
authors.initials: XY
authors.lastname: Cao
authors.affiliation: Idaho State University, Department of Biological Sciences, Pocatello, 83209, USA.
authors.forename: Jack
authors.initials: J
authors.lastname: Rose
authors.affiliation: Institute of Special Animal and Plant Sciences, Chinese Academy of Agricultural Sciences, No.4899 Juye Street, Jingyue District, Changchun 130112, China.|State Key Laboratory for Molecular Biology of Special Economic Animal and Plant Science, Chinese Academy of Agricultural Sciences, Changchun 130112, P. R. China.
authors.forename: Shi-Yong
authors.initials: SY
authors.lastname: Wang
authors.affiliation: Key Laboratory of Embryo Development and Reproductive Regulation of Anhui Province, College of Biological and Food Engineering, Fuyang Teachers College, Fuyang, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Institute of Special Animal and Plant Sciences, Chinese Academy of Agricultural Sciences, No.4899 Juye Street, Jingyue District, Changchun 130112, China.|State Key Laboratory for Molecular Biology of Special Economic Animal and Plant Science, Chinese Academy of Agricultural Sciences, Changchun 130112, P. R. China.
authors.forename: Meng
authors.initials: M
authors.lastname: Zhao
authors.affiliation: Institute of Special Animal and Plant Sciences, Chinese Academy of Agricultural Sciences, No.4899 Juye Street, Jingyue District, Changchun 130112, China.|State Key Laboratory for Molecular Biology of Special Economic Animal and Plant Science, Chinese Academy of Agricultural Sciences, Changchun 130112, P. R. China.
authors.forename: Ming-Jie
authors.initials: MJ
authors.lastname: Xing
authors.affiliation: Institute of Special Animal and Plant Sciences, Chinese Academy of Agricultural Sciences, No.4899 Juye Street, Jingyue District, Changchun 130112, China.|State Key Laboratory for Molecular Biology of Special Economic Animal and Plant Science, Chinese Academy of Agricultural Sciences, Changchun 130112, P. R. China.
authors.forename: Tong
authors.initials: T
authors.lastname: Chang
authors.affiliation: Institute of Special Animal and Plant Sciences, Chinese Academy of Agricultural Sciences, No.4899 Juye Street, Jingyue District, Changchun 130112, China.|State Key Laboratory for Molecular Biology of Special Economic Animal and Plant Science, Chinese Academy of Agricultural Sciences, Changchun 130112, P. R. China.
authors.forename: Baozeng
authors.initials: B
authors.lastname: Xu
mesh_terms: D000818:Animals; D001755:Blastocyst / Q000166:cytology / Q000378:metabolism*; D015925:Cryopreservation; D003451:Cryoprotective Agents / Q000494:pharmacology*; D047108:Embryonic Development; D005260:Female; D005307:Fertilization in Vitro; D005998:Glycine / Q000494:pharmacology*; D051379:Mice; D009865:Oocytes / Q000166:cytology / Q000378:metabolism*
chemical_list: D003451:Cryoprotective Agents; D005998:Glycine