title: Cloning and characterization of decaprenyl diphosphate synthase from three different fungi.
abstract: Coenzyme Q (CoQ) is composed of a benzoquinone moiety and an isoprenoid side chain of varying lengths. The length of the side chain is controlled by polyprenyl diphosphate synthase. In this study, dps1 genes encoding decaprenyl diphosphate synthase were cloned from three fungi: Bulleromyces albus, Saitoella complicata, and Rhodotorula minuta. The predicted Dps1 proteins contained seven conserved domains found in typical polyprenyl diphosphate synthases and were 528, 440, and 537 amino acids in length in B. albus, S. complicata, and R. minuta, respectively. Escherichia coli expressing the fungal dps1 genes produced CoQ10in addition to endogenous CoQ8. Two of the three fungal dps1 genes (from S. complicata and R. minuta) were able to replace the function of ispB in an E. coli mutant strain. In vitro enzymatic activities were also detected in recombinant strains. The three dps1 genes were able to complement a Schizosaccharomyces pombe dps1, dlp1 double mutant. Recombinant S. pombe produced mainly CoQ10, indicating that the introduced genes were independently functional and did not require dlp1. The cloning of dps1 genes from various fungi has the potential to enhance production of CoQ10in other organisms.
authors.affiliation: Kaneka Corporation, 1-8, Miyamae-cho, Takasago-cho, Takasago, Hyogo, 676-8688, Japan.|Department of Life Science and Biotechnology, Faculty of Life and Environmental Science, Shimane University, 1060 Nishikawatsu, Matsue, 690-8504, Japan.
authors.forename: Daisuke
authors.initials: D
authors.lastname: Moriyama
authors.affiliation: Department of Life Science and Biotechnology, Faculty of Life and Environmental Science, Shimane University, 1060 Nishikawatsu, Matsue, 690-8504, Japan.
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Kaino
authors.affiliation: Kaneka Corporation, 1-8, Miyamae-cho, Takasago-cho, Takasago, Hyogo, 676-8688, Japan.
authors.forename: Kazuyoshi
authors.initials: K
authors.lastname: Yajima
authors.affiliation: Department of Life Science and Biotechnology, Faculty of Life and Environmental Science, Shimane University, 1060 Nishikawatsu, Matsue, 690-8504, Japan.
authors.forename: Ryota
authors.initials: R
authors.lastname: Yanai
authors.affiliation: Kaneka Corporation, 1-8, Miyamae-cho, Takasago-cho, Takasago, Hyogo, 676-8688, Japan.
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Ikenaka
authors.affiliation: Kaneka Corporation, 1-8, Miyamae-cho, Takasago-cho, Takasago, Hyogo, 676-8688, Japan.
authors.forename: Junzo
authors.initials: J
authors.lastname: Hasegawa
authors.affiliation: Kaneka Corporation, 1-8, Miyamae-cho, Takasago-cho, Takasago, Hyogo, 676-8688, Japan.
authors.forename: Motohisa
authors.initials: M
authors.lastname: Washida
authors.affiliation: Kaneka Corporation, 1-8, Miyamae-cho, Takasago-cho, Takasago, Hyogo, 676-8688, Japan.
authors.forename: Hirokazu
authors.initials: H
authors.lastname: Nanba
authors.affiliation: Department of Life Science and Biotechnology, Faculty of Life and Environmental Science, Shimane University, 1060 Nishikawatsu, Matsue, 690-8504, Japan. kawamuka@life.shimane-u.ac.jp.
authors.forename: Makoto
authors.initials: M
authors.lastname: Kawamukai
mesh_terms: D019883:Alkyl and Aryl Transferases / Q000235:genetics / Q000378:metabolism*; D005658:Fungi / Q000201:enzymology*; D012568:Schizosaccharomyces / Q000201:enzymology; D014451:Ubiquinone / Q000378:metabolism
chemical_list: D014451:Ubiquinone; D019883:Alkyl and Aryl Transferases; C039821:decaprenyl pyrophosphate synthetase
keywords: CoQ; Coenzyme Q; Decaprenyl diphosphate synthase; Fission yeast; Ubiquinone