title: Production of gold nanoparticles by electrode-respiring Geobacter sulfurreducens biofilms.
abstract: The goal of this work was to synthesize gold nanoparticles (AuNPs) using electrode-respiring Geobacter sulfurreducens biofilms. We found that AuNPs are generated in the extracellular matrix of Geobacter biofilms and have an average particle size of 20nm. The formation of AuNPs was verified using TEM, FTIR and EDX. We also found that the extracellular substances extracted from electrode-respiring G. sulfurreducens biofilms reduce Au3+to AuNPs. From FTIR spectra, it appears that reduced sugars were involved in the bioreduction and synthesis of AuNPs and that amine groups acted as the major biomolecules involved in binding.
authors.affiliation: The Gene and Linda Voiland School of Chemical Engineering and Bioengineering, Washington State University, Pullman, WA, USA.
authors.forename: Abid H
authors.initials: AH
authors.lastname: Tanzil
authors.affiliation: The Gene and Linda Voiland School of Chemical Engineering and Bioengineering, Washington State University, Pullman, WA, USA.
authors.forename: Sujala T
authors.initials: ST
authors.lastname: Sultana
authors.affiliation: The Gene and Linda Voiland School of Chemical Engineering and Bioengineering, Washington State University, Pullman, WA, USA.
authors.forename: Steven R
authors.initials: SR
authors.lastname: Saunders
authors.affiliation: Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory, Richland, WA, USA.
authors.forename: Alice C
authors.initials: AC
authors.lastname: Dohnalkova
authors.affiliation: Biological Sciences Division, Pacific Northwest National Laboratory, Richland, WA, USA.
authors.forename: Liang
authors.initials: L
authors.lastname: Shi
authors.affiliation: The Gene and Linda Voiland School of Chemical Engineering and Bioengineering, Washington State University, Pullman, WA, USA.
authors.forename: Emily
authors.initials: E
authors.lastname: Davenport
authors.affiliation: The Gene and Linda Voiland School of Chemical Engineering and Bioengineering, Washington State University, Pullman, WA, USA.
authors.forename: Phuc
authors.initials: P
authors.lastname: Ha
authors.affiliation: The Gene and Linda Voiland School of Chemical Engineering and Bioengineering, Washington State University, Pullman, WA, USA. Electronic address: beyenal@wsu.edu.
authors.forename: Haluk
authors.initials: H
authors.lastname: Beyenal
mesh_terms: D018441:Biofilms; D001709:Biotechnology; D055664:Electrochemical Techniques; D005109:Extracellular Matrix / Q000378:metabolism; D045302:Geobacter / Q000378:metabolism*; D006046:Gold / Q000737:chemistry*; D055772:Green Chemistry Technology; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D046529:Microscopy, Electron, Transmission; D036103:Nanotechnology; D010084:Oxidation-Reduction; D010316:Particle Size; D013052:Spectrometry, X-Ray Emission; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D006046:Gold
keywords: Biofilms; Extracellular substances; Geobacter sulfurreducens; Gold nanoparticles