title: The effects of ropivacaine hydrochloride on the expression of CaMK II mRNA in the dorsal root ganglion neurons.
abstract: In this study, we identified the subtype of Calcium/calmodulin-dependent protein kinase II (CaMK II) mRNA in dorsal root ganglion neurons and observed the effects of ropivacaine hydrochloride in different concentration and different exposure time on the mRNA expression. Dorsal root ganglion neurons were isolated from the SD rats and cultured in vitro. The mRNA of the CaMK II subtype in dorsal root ganglion neurons were detected by real-time PCR. As well as, the dorsal root ganglion neurons were treated with ropivacaine hydrochloride in different concentration (1mM,2mM, 3mM and 4mM) for the same exposure time of 4h, or different exposure time (0h,2h,3h,4h and 6h) at the same concentration(3mM). The changes of the mRNA expression of the CaMK II subtype were observed with real-time PCR. All subtype mRNA of the CaMK II, CaMK IIα, CaMK IIβ, CaMK IIδ, CaMK IIγ, can be detected in dorsal root ganglion neurons. With the increased of the concentration and exposure time of the ropivacaine hydrochloride, all the subtype mRNA expression increased. Ropivacaine hydrochloride up-regulate the CaMK IIβ, CaMK IIδ, CaMK IIgmRNA expression with the concentration and exposure time increasing. The nerve blocking or the neurotoxicity of the ropivacaine hydrochloride maybe involved with CaMK II.
authors.affiliation: Department of Anesthesiology, The First People's Hospital of Foshan & Foshan Hospital of Sun Yat-sen University, Foshan 528000, Guangdong Province, China. Electronic address: xjwen166@163.com.
authors.forename: Xianjie
authors.initials: X
authors.lastname: Wen
authors.affiliation: Department of Anesthesiology, The First People's Hospital of Foshan & Foshan Hospital of Sun Yat-sen University, Foshan 528000, Guangdong Province, China. Electronic address: lxhong@fsyyy.com.
authors.forename: Xiaohong
authors.initials: X
authors.lastname: Lai
authors.affiliation: Department of Anesthesiology, The First People's Hospital of Foshan & Foshan Hospital of Sun Yat-sen University, Foshan 528000, Guangdong Province, China.
authors.forename: Xiaohong
authors.initials: X
authors.lastname: Li
authors.affiliation: Department of Anesthesiology, The First People's Hospital of Foshan & Foshan Hospital of Sun Yat-sen University, Foshan 528000, Guangdong Province, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Zhang
authors.affiliation: Department of Anesthesiology, The First People's Hospital of Foshan & Foshan Hospital of Sun Yat-sen University, Foshan 528000, Guangdong Province, China.
authors.forename: Hua
authors.initials: H
authors.lastname: Liang
mesh_terms: D000577:Amides / Q000494:pharmacology*; D000779:Anesthetics, Local / Q000494:pharmacology*; D000818:Animals; D000831:Animals, Newborn; D054732:Calcium-Calmodulin-Dependent Protein Kinase Type 2 / Q000096:biosynthesis* / Q000235:genetics; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D005727:Ganglia, Spinal / Q000187:drug effects / Q000201:enzymology*; D015971:Gene Expression Regulation, Enzymologic; D009474:Neurons / Q000187:drug effects / Q000201:enzymology*; D012333:RNA, Messenger / Q000096:biosynthesis* / Q000235:genetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D000077212:Ropivacaine
chemical_list: D000577:Amides; D000779:Anesthetics, Local; D012333:RNA, Messenger; D000077212:Ropivacaine; D054732:Calcium-Calmodulin-Dependent Protein Kinase Type 2
keywords: Calcium/calmodulin-dependent protein kinase II; Dorsal root ganglion neurons; Local anesthetics; Ropivacaine hydrochloride