title: The vasorelaxant mechanisms of methanol on isolated rat aortic rings: Involvement of ion channels and signal transduction pathways.
abstract: It is reported that methanol is generally used as an industrial solvent, antifreeze, windshield washer fluid, cooking fuel and perfume. Methanol ingestion can lead to severe metabolic disturbances, blindness, or even death. So far, few studies about its negative effects on cardiovascular system have been reported. The purpose of this study was to determine the vasoactive effect of methanol and roles of ion channels and signal transduction pathways on isolated rat aorta. The results suggested that the mechanism of methanol-induced vasorelaxation at low concentrations (<500 mM) was mediated by ATP-sensitive K+(KATP) and L-type Ca2+channels, but the mechanism at high concentrations (>600 mM) was related to KATP, voltage-dependent K+, big-conductance Ca2+-activated K+, L-type Ca2+channels as well as prostacyclin, protein kinase C, β-adrenoceptors pathways. In addition, methanol induced a dose-dependent inhibition of vasoconstrictions caused by calcium chloride, potassium chloride, or norepinephrine. Further work is needed to investigate the relative contribution of each channel and pathway in methanol-induced vasoactive effect.
authors.affiliation: 1 Institute of Environmental Medicine and Toxicology, Institute of Environmental Science, Shanxi University, Taiyuan, People's Republic of China.
authors.forename: Y
authors.initials: Y
authors.lastname: Bai
authors.affiliation: 1 Institute of Environmental Medicine and Toxicology, Institute of Environmental Science, Shanxi University, Taiyuan, People's Republic of China.
authors.forename: Q
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: 1 Institute of Environmental Medicine and Toxicology, Institute of Environmental Science, Shanxi University, Taiyuan, People's Republic of China.
authors.forename: Z
authors.initials: Z
authors.lastname: Yang
authors.affiliation: 1 Institute of Environmental Medicine and Toxicology, Institute of Environmental Science, Shanxi University, Taiyuan, People's Republic of China.
authors.forename: Z
authors.initials: Z
authors.lastname: Meng
authors.affiliation: 2 State Key Laboratory of Environmental Chemistry and Eco-toxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing, People's Republic of China.
authors.forename: Q
authors.initials: Q
authors.lastname: Zhao
mesh_terms: D000818:Animals; D001013:Aorta, Thoracic / Q000187:drug effects* / Q000502:physiology; D020746:Calcium Channels, L-Type / Q000502:physiology; D011464:Epoprostenol / Q000502:physiology; D066298:In Vitro Techniques; D054086:KATP Channels / Q000502:physiology; D051036:Large-Conductance Calcium-Activated Potassium Channels / Q000502:physiology; D008297:Male; D000432:Methanol / Q000633:toxicity*; D009638:Norepinephrine; D011189:Potassium Chloride; D011493:Protein Kinase C / Q000502:physiology; D017208:Rats, Wistar; D011943:Receptors, Adrenergic, beta / Q000502:physiology; D012997:Solvents / Q000633:toxicity*; D014661:Vasoconstriction / Q000187:drug effects; D014665:Vasodilator Agents / Q000633:toxicity*
chemical_list: D020746:Calcium Channels, L-Type; D054086:KATP Channels; D051036:Large-Conductance Calcium-Activated Potassium Channels; D011943:Receptors, Adrenergic, beta; D012997:Solvents; D014665:Vasodilator Agents; D011189:Potassium Chloride; D011464:Epoprostenol; D011493:Protein Kinase C; D009638:Norepinephrine; D000432:Methanol
keywords: Methanol; aortic rings; ion channels; signal transduction pathways