title: Nanosized mesoporous metal-organic framework MIL-101 as a nanocarrier for photoactive hexamolybdenum cluster compounds.
abstract: Inclusion compounds of photoluminescent hexamolybdenum cluster complexes in the chromium terephthalate metal-organic framework, MIL-101 (MIL, Matérial Institut Lavoisier) were successfully synthesized in two different ways and characterized by means of powder X-Ray diffraction, chemical analysis and nitrogen sorption. Some important functional properties of hexamolybdenum cluster complexes for biological and medical applications, in particular singlet oxygen generation ability, luminescence properties, cellular uptake behavior and cytotoxicity were studied. It was revealed that the inclusion compounds possessed significant singlet oxygen generation activity. The materials obtained showed a low cytotoxicity, thus allowing them to be used in living cells. Confocal microscopy of human larynx carcinoma (Hep-2) cells incubated with the inclusion compounds showed that MIL-101 performed as a nanocarrier adhering to the external cell membrane surface and releasing the cluster complexes which that penetrated into the cells. Moreover, photoinduced generation of reactive oxygen species (ROS) in Hep-2 cells incubated with inclusion compounds was demonstrated. The cluster supported on MIL-101 was shown to possess in vivo phototoxicity.
authors.affiliation: Nikolaev Institute of Inorganic Chemistry SB RAS, 3 Acad. Lavrentiev ave., 630090 Novosibirsk, Russian Federation; Novosibirsk State University, 2 Pirogova st., 630090 Novosibirsk, Russian Federation.
authors.forename: Anastasia M
authors.initials: AM
authors.lastname: Cheplakova
authors.affiliation: Scientific Institute of Clinical and Experimental Lymphology, 2 Timakova st., 630060 Novosibirsk, Russian Federation.
authors.forename: Anastasiya O
authors.initials: AO
authors.lastname: Solovieva
authors.affiliation: Novosibirsk State University, 2 Pirogova st., 630090 Novosibirsk, Russian Federation; Scientific Institute of Clinical and Experimental Lymphology, 2 Timakova st., 630060 Novosibirsk, Russian Federation.
authors.forename: Tatiana N
authors.initials: TN
authors.lastname: Pozmogova
authors.affiliation: Nikolaev Institute of Inorganic Chemistry SB RAS, 3 Acad. Lavrentiev ave., 630090 Novosibirsk, Russian Federation.
authors.forename: Yuri A
authors.initials: YA
authors.lastname: Vorotnikov
authors.affiliation: Nikolaev Institute of Inorganic Chemistry SB RAS, 3 Acad. Lavrentiev ave., 630090 Novosibirsk, Russian Federation; Novosibirsk State University, 2 Pirogova st., 630090 Novosibirsk, Russian Federation; Scientific Institute of Clinical and Experimental Lymphology, 2 Timakova st., 630060 Novosibirsk, Russian Federation.
authors.forename: Konstantin A
authors.initials: KA
authors.lastname: Brylev
authors.affiliation: Nikolaev Institute of Inorganic Chemistry SB RAS, 3 Acad. Lavrentiev ave., 630090 Novosibirsk, Russian Federation.
authors.forename: Natalya A
authors.initials: NA
authors.lastname: Vorotnikova
authors.affiliation: The Institute of Molecular Biology and Biophysics, 2/12 Timakova st., 630117 Novosibirsk, Russian Federation.
authors.forename: Elena V
authors.initials: EV
authors.lastname: Vorontsova
authors.affiliation: Nikolaev Institute of Inorganic Chemistry SB RAS, 3 Acad. Lavrentiev ave., 630090 Novosibirsk, Russian Federation; Novosibirsk State University, 2 Pirogova st., 630090 Novosibirsk, Russian Federation.
authors.forename: Yuri V
authors.initials: YV
authors.lastname: Mironov
authors.affiliation: Scientific Institute of Clinical and Experimental Lymphology, 2 Timakova st., 630060 Novosibirsk, Russian Federation.
authors.forename: Alexander F
authors.initials: AF
authors.lastname: Poveshchenko
authors.affiliation: Nikolaev Institute of Inorganic Chemistry SB RAS, 3 Acad. Lavrentiev ave., 630090 Novosibirsk, Russian Federation; Novosibirsk State University, 2 Pirogova st., 630090 Novosibirsk, Russian Federation. Electronic address: k.a.kovalenko@niic.nsc.ru.
authors.forename: Konstantin A
authors.initials: KA
authors.lastname: Kovalenko
authors.affiliation: Nikolaev Institute of Inorganic Chemistry SB RAS, 3 Acad. Lavrentiev ave., 630090 Novosibirsk, Russian Federation; Novosibirsk State University, 2 Pirogova st., 630090 Novosibirsk, Russian Federation; Scientific Institute of Clinical and Experimental Lymphology, 2 Timakova st., 630060 Novosibirsk, Russian Federation. Electronic address: shtopy@niic.nsc.ru.
authors.forename: Michael A
authors.initials: MA
authors.lastname: Shestopalov
mesh_terms: D000970:Antineoplastic Agents / Q000737:chemistry / Q000494:pharmacology; D002460:Cell Line; D056831:Coordination Complexes / Q000737:chemistry / Q000494:pharmacology; D004337:Drug Carriers / Q000737:chemistry / Q000494:pharmacology; D006801:Humans; D007822:Laryngeal Neoplasms / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D000073396:Metal-Organic Frameworks; D008982:Molybdenum / Q000737:chemistry / Q000494:pharmacology; D017382:Reactive Oxygen Species / Q000378:metabolism
chemical_list: D000970:Antineoplastic Agents; D056831:Coordination Complexes; D004337:Drug Carriers; C000589635:MIL-101; D000073396:Metal-Organic Frameworks; D017382:Reactive Oxygen Species; D008982:Molybdenum
keywords: Hep-2 cells; Luminescence; MIL-101; Molybdenum clusters; Phototoxicity; Singlet oxygen