title: Single-Cell Sequencing for Drug Discovery and Drug Development.
abstract: Next-generation sequencing (NGS), particularly single-cell sequencing, has revolutionized the
  scale and scope of genomic and biomedical research. Recent technological advances in NGS and singlecell
  studies have made the deep whole-genome (DNA-seq), whole epigenome and whole-transcriptome
  sequencing (RNA-seq) at single-cell level feasible. NGS at the single-cell level expands our view of
  genome, epigenome and transcriptome and allows the genome, epigenome and transcriptome of any
  organism to be explored without a priori assumptions and with unprecedented throughput. And it does
  so with single-nucleotide resolution. NGS is also a very powerful tool for drug discovery and drug development.
  In this review, we describe the current state of single-cell sequencing techniques, which can
  provide a new, more powerful and precise approach for analyzing effects of drugs on treated cells and
  tissues. Our review discusses single-cell whole genome/exome sequencing (scWGS/scWES), single-cell
  transcriptome sequencing (scRNA-seq), single-cell bisulfite sequencing (scBS), and multiple omics of
  single-cell sequencing. We also highlight the advantages and challenges of each of these approaches.
  Finally, we describe, elaborate and speculate the potential applications of single-cell sequencing for drug
  discovery and drug development.
authors.affiliation: Cancer Research Institute, Hangzhou Cancer Hospital, Hangzhou, 320000, Zhejiang Province, P.R. China|Center for Genomics & Department of Basic Sciences, School of Medicine, Loma Linda University, Loma Linda, CA 92350, USA
authors.forename: Hongjin
authors.initials: H
authors.lastname: Wu
authors.affiliation: Center for Genomics & Department of Basic Sciences, School of Medicine, Loma Linda University, Loma Linda, CA 92350, USA
authors.forename: Charles
authors.initials: C
authors.lastname: Wang
authors.affiliation: Cancer Research Institute, Hangzhou Cancer Hospital, Hangzhou, 320000, Zhejiang Province, P.R. China
authors.forename: Shixiu
authors.initials: S
authors.lastname: Wu
mesh_terms: D055808:Drug Discovery; D006801:Humans; D017422:Sequence Analysis, DNA; D059010:Single-Cell Analysis
keywords: Drug discovery; NGS; RNA-seq; Single-cell bisulfite sequencing; Single-cell sequencing