title: In vitroandIn vivoAntioxidant, Anti-hyperlipidemic Properties and Chemical Characterization ofCentella asiatica(L.) Extract.
abstract: The study aimed to identify the phenolic compounds present inCentella asiatica(L.) (C. asiatica) extract and evaluate the respective antioxidant potential as well as its cholesterol-lowering effects in the experimental animal model. Herein, the antioxidant potential of extracts was assessed by its free radical scavenging activity such as 2, 2-diphenyl -1- picrylhydrazyl as well as reducing capability. The anti-hyperlipidemic effects ofC. asiaticaextract (CAE) were evaluated in high cholesterol-fed (HCF) rats for 4 weeks, where different concentrations of extracts (0.25, 0.5, and 1 g/kg/day) were orally administrated daily. Lipid and antioxidant profiles, including total cholesterol (TC), triglyceride (TG), low density lipoprotein cholesterol (LDL-C), high density lipoprotein cholesterol (HDL-C) and superoxide dismutase (SOD), together with the indices of hepatic functions were also examined.C. asiaticarevealed excellent free radical scavenging activity as revealed by 2-2- diphenyl-1-picryl-hydrazyl (DPPH) assay, with the IC50values (9.62 ± 0.88 μg/mL). Furthermore,C. asiaticaextracts and fenofibrate remarkably lowered the level of TC, TG, LDL-C, and showed elevated levels of HDL-C, SOD. The histopathological observations further demonstrated clear differentiation and structural changes in liver of HCF and CAE treated group. Furthermore, gulonic acid, ferulic acid, kaempferol, chlorogenic acid, and asiatic acid were identified to be the major components which might be responsible for the antioxidant activity of theC. asiaticaextract as evidenced from an ultra-high performance liquid chromatography-mass spectrometer. Taken together, these results signifies the excellent antioxidant and anti-hyperlipidemic properties ofC. asiaticaleaf extracts, which might be useful for the treatment of oxidative-stress related diseases such as hyperlipidemia.
authors.affiliation: Biochemistry Laboratory, Life Sciences Division, Institute of Advanced Study in Science and Technology Guwahati, India.
authors.forename: Sima
authors.initials: S
authors.lastname: Kumari
authors.affiliation: Biochemistry Laboratory, Life Sciences Division, Institute of Advanced Study in Science and Technology Guwahati, India.
authors.forename: Meetali
authors.initials: M
authors.lastname: Deori
authors.affiliation: Drug Discovery Laboratory, Life Sciences Division, Institute of Advanced Study in Science and Technology Guwahati, India.
authors.forename: R
authors.initials: R
authors.lastname: Elancheran
authors.affiliation: Drug Discovery Laboratory, Life Sciences Division, Institute of Advanced Study in Science and Technology Guwahati, India.
authors.forename: Jibon
authors.initials: J
authors.lastname: Kotoky
authors.affiliation: Biochemistry Laboratory, Life Sciences Division, Institute of Advanced Study in Science and Technology Guwahati, India.
authors.forename: Rajlakshmi
authors.initials: R
authors.lastname: Devi
keywords: Centella asiatica; UHPLC–MS/MS; anti-hyperlipidemic; antioxidant; medicinal plants