title: Comparison of monoplex and duplex RT-PCR assays for the detection of measles virus.
abstract: Rapid and accurate detection of measles virus is important for case diagnosis and public health management. This study compared the performance of two monoplex RT-PCR reactions targeting the H and N genes to a duplex RT-PCR targeting both genes simultaneously. The duplex simplified processing without compromising assay performance characteristic.
authors.affiliation: Nova Scotia Health Authority (NSHA) and Dalhousie University, Halifax, NS, Canada.
authors.forename: Khalifa
authors.initials: K
authors.lastname: Binkhamis
authors.affiliation: Nova Scotia Health Authority (NSHA) and Dalhousie University, Halifax, NS, Canada.
authors.forename: Hayley
authors.initials: H
authors.lastname: Gillis
authors.affiliation: Nova Scotia Health Authority (NSHA) and Dalhousie University, Halifax, NS, Canada.
authors.forename: Joseph Daniel
authors.initials: JD
authors.lastname: Lafreniere
authors.affiliation: Viral Exanthemata and STDs, National Microbiology Laboratory (NML), Public Health Agency of Canada (PHAC), Winnipeg, MB, Canada.
authors.forename: Joanne
authors.initials: J
authors.lastname: Hiebert
authors.affiliation: Viral Exanthemata and STDs, National Microbiology Laboratory (NML), Public Health Agency of Canada (PHAC), Winnipeg, MB, Canada.
authors.forename: Lillian
authors.initials: L
authors.lastname: Mendoza
authors.affiliation: Nova Scotia Provincial Public Health Laboratory Network (PPHLN), Halifax, NS, Canada.
authors.forename: Janice
authors.initials: J
authors.lastname: Pettipas
authors.affiliation: Viral Exanthemata and STDs, National Microbiology Laboratory (NML), Public Health Agency of Canada (PHAC), Winnipeg, MB, Canada.
authors.forename: Alberto
authors.initials: A
authors.lastname: Severini
authors.affiliation: Nova Scotia Health Authority (NSHA) and Dalhousie University, Halifax, NS, Canada.
authors.forename: Todd F
authors.initials: TF
authors.lastname: Hatchette
authors.affiliation: Nova Scotia Health Authority (NSHA) and Dalhousie University, Halifax, NS, Canada. Electronic address: jason.leblanc@nshealth.ca.
authors.forename: Jason J
authors.initials: JJ
authors.lastname: LeBlanc
mesh_terms: D002170:Canada / Q000453:epidemiology; D017931:DNA Primers; D006389:Hemagglutinins, Viral / Q000235:genetics; D006801:Humans; D008457:Measles / Q000175:diagnosis* / Q000453:epidemiology / Q000821:virology; D008459:Measles virus / Q000235:genetics / Q000302:isolation & purification*; D025202:Molecular Diagnostic Techniques; D060885:Multiplex Polymerase Chain Reaction; D009439:Neuraminidase / Q000235:genetics; D011634:Public Health; D012367:RNA, Viral / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012680:Sensitivity and Specificity
chemical_list: D017931:DNA Primers; D006389:Hemagglutinins, Viral; D012367:RNA, Viral; D009439:Neuraminidase
keywords: Canada; Hemagglutinin; Measles; Method; Molecular; Neuraminidase; PCR