title: Rubricella aquisinus gen. nov., sp. nov., a novel member of the family Rhodobacteraceae.
abstract: A Gram-stain negative, ovoid or short rod-shaped, aerobic and non-motile bacterial strain, designated J82T, was isolated from a seawater sample collected from the coast of Yellow Sea in Qingdao, China. The strain grew at salinities of 1.0-6.0% (w/v) NaCl (optimum, 2.5%). Growth occurred at pH 6.0-8.0 (optimum, pH 7.0) and 10-42 °C (optimum, 28-30 °C). The genomic DNA G + C content was determined to be 57.5 mol%. Q-10 was detected as the respiratory quinone. The major fatty acid (>10%) was Summed feature 8 (C18:1ω7c and/or C18:1ω6c). The polar lipids consisted of phosphatidylethanolamine, two unidentified aminolipids and two unidentified polar lipids. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain J82Tforms a distinct evolutionary lineage within the family Rhodobacteraceae. On the basis of phenotypic, chemotaxonomic and phylogenetic characteristics, the strain merits recognition as representative of a novel genus and species within the family Rhodobacteraceae for which the name Rubricella aquisinus gen. nov., sp. nov. is proposed. The type strain of Rubricella aquisinus is J82T(= DSM 103377T= CCTCC AB 2016170T).
authors.affiliation: Research Center for Marine Biology and Carbon Sequestration, Shandong Provincial Key Laboratory of Energy Genetics, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, 266101, People's Republic of China.
authors.forename: Li-Qiang
authors.initials: LQ
authors.lastname: Yang
authors.affiliation: Research Center for Marine Biology and Carbon Sequestration, Shandong Provincial Key Laboratory of Energy Genetics, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, 266101, People's Republic of China.
authors.forename: Lili
authors.initials: L
authors.lastname: Tang
authors.affiliation: State Key Laboratory of Biocontrol and Guangdong Provincial Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat-Sen University, Guangzhou, 510275, People's Republic of China.
authors.forename: Lan
authors.initials: L
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Biocontrol and Guangdong Provincial Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat-Sen University, Guangzhou, 510275, People's Republic of China.
authors.forename: Nimaichand
authors.initials: N
authors.lastname: Salam
authors.affiliation: State Key Laboratory of Biocontrol and Guangdong Provincial Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat-Sen University, Guangzhou, 510275, People's Republic of China. liwenjun3@mail.sysu.edu.cn.
authors.forename: Wen-Jun
authors.initials: WJ
authors.lastname: Li
authors.affiliation: CNOOC China Limited, Zhanjiang Branch, Zhanjiang, 524057, People's Republic of China.
authors.forename: Xiaowei
authors.initials: X
authors.lastname: Liu
authors.affiliation: CNOOC China Limited, Zhanjiang Branch, Zhanjiang, 524057, People's Republic of China.
authors.forename: Guangzhi
authors.initials: G
authors.lastname: Jin
authors.affiliation: Institute of Marine Microbes and Ecospheres, State Key Laboratory of Marine Environmental Science, Xiamen University, Xiamen, 361101, People's Republic of China.
authors.forename: Nianzhi
authors.initials: N
authors.lastname: Jiao
authors.affiliation: Research Center for Marine Biology and Carbon Sequestration, Shandong Provincial Key Laboratory of Energy Genetics, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, 266101, People's Republic of China. zhangyy@qibebt.ac.cn.
authors.forename: Yongyu
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D002681:China; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D006863:Hydrogen-Ion Concentration; D010641:Phenotype; D010743:Phospholipids / Q000032:analysis; D010802:Phylogeny; D011809:Quinones / Q000032:analysis; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D041902:Rhodobacteraceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D054712:Salinity; D012623:Seawater / Q000382:microbiology*; D012965:Sodium Chloride / Q000032:analysis; D013045:Species Specificity; D013696:Temperature; D014451:Ubiquinone / Q000032:analysis
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D010743:Phospholipids; D011809:Quinones; D012336:RNA, Ribosomal, 16S; D014451:Ubiquinone; D012965:Sodium Chloride
keywords: Family Rhodobacteraceae; Polyphasic taxonomy; Rubricella aquisinus gen. nov., sp. nov.; Yellow Sea