title: Effects of high-sodium intake on systemic blood pressure and vascular responses in spontaneously diabetic WBN/Kob-Leprfa/farats.
abstract: The prevalence of type 2 diabetes mellitus (T2DM) and hypertension has markedly increased worldwide. The purpose of the present study was to examine the effects of a high-salt intake on the systolic blood pressure (SBP) and vascular responses in WBN/Kob-Leprfa/fa(WBKDF) rats, a new spontaneous animal model of T2DM. Male WBKDF rats and age-matched Wistar rats at 6 weeks of age were each divided into two groups and fed either a normal-sodium (NS, 0.26%) diet or high-sodium (HS, 8%) diet for 14 weeks: (i) Wistar rats on NS diet (Wistar-NS); (ii) Wistar rats on HS diet (Wistar-HS); (iii) WBKDF rats on NS diet (WBKDF-NS); (iv) WBKDF rats on HS diets (WBKDF-HS). Neither WBKDF-NS nor Wistar-NS rats showed significant changes in SBP throughout the experiment, but both WBKDF-HS and Wistar-HS exhibited significant elevation of SBP, which was more prominent (P<.01) in WBKDF-HS than in Wistar-HS. Phenylephrine-induced contractions of isolated thoracic aortic rings were significantly (P<.01) enhanced in WBKDF-HS and Wistar-HS compared with the respective strain of rats on the NS diet. In contrast, acetylcholine- and nitroprusside-induced relaxation were significantly (P<.01) diminished in both WBKDF-HS and Wistar-HS, and these HS diet-induced changes were more profound (P<.01) in WBKDF rats than in Wistar rats. Significantly (P<.05) higher plasma concentrations of 8-iso-prostaglandin F2αand sodium ions were observed in WBKDF-HS than in Wistar-HS. The current study demonstrated that WBKDF-HS rats developed salt-sensitive hypertension associated with vascular dysfunction. The WBKDF rat may be a useful model for investigating the etiology of hypertension with T2DM.
authors.affiliation: Laboratory of Veterinary Pharmacology, Azabu University, Sagamihara, Kanagawa, Japan.
authors.forename: Yoshiichi
authors.initials: Y
authors.lastname: Takagi
authors.affiliation: Laboratory of Veterinary Pharmacology, Azabu University, Sagamihara, Kanagawa, Japan.
authors.forename: Haruno
authors.initials: H
authors.lastname: Kadowaki
authors.affiliation: Laboratory of Veterinary Pharmacology, Azabu University, Sagamihara, Kanagawa, Japan.
authors.forename: Ikumi
authors.initials: I
authors.lastname: Kobayashi
authors.affiliation: Clinical Nutrition, Chiba University Hospital, Chiba, Japan.
authors.forename: Kaoru
authors.initials: K
authors.lastname: Ito
authors.affiliation: Laboratory of Veterinary Pharmacology, Azabu University, Sagamihara, Kanagawa, Japan.
authors.forename: Katsuaki
authors.initials: K
authors.lastname: Ito
authors.affiliation: Laboratory of Veterinary Pharmacology, Azabu University, Sagamihara, Kanagawa, Japan.
authors.forename: Mitsuyuki
authors.initials: M
authors.lastname: Shirai
authors.affiliation: Laboratory of Veterinary Pharmacology, Azabu University, Sagamihara, Kanagawa, Japan.
authors.forename: Fumitoshi
authors.initials: F
authors.lastname: Asai
mesh_terms: D000818:Animals; D020296:Animals, Congenic; D001013:Aorta, Thoracic / Q000187:drug effects / Q000503:physiopathology; D001786:Blood Glucose / Q000032:analysis; D001794:Blood Pressure / Q000187:drug effects*; D003921:Diabetes Mellitus, Experimental / Q000097:blood / Q000150:complications / Q000503:physiopathology*; D003924:Diabetes Mellitus, Type 2 / Q000097:blood / Q000150:complications / Q000503:physiopathology*; D006973:Hypertension / Q000209:etiology; D007328:Insulin / Q000097:blood; D007668:Kidney / Q000473:pathology / Q000503:physiopathology; D008297:Male; D009765:Obesity / Q000097:blood / Q000150:complications / Q000503:physiopathology*; D017208:Rats, Wistar; D017673:Sodium Chloride, Dietary / Q000009:adverse effects*; D014661:Vasoconstriction / Q000187:drug effects*
chemical_list: D001786:Blood Glucose; D007328:Insulin; D017673:Sodium Chloride, Dietary
keywords: WBN/Kob-Leprfa/fa rat; high-sodium diet; salt-sensitive hypertension; type 2 diabetes mellitus