title: Influence of calcium salts and bovine thrombin on growth factor release from equine platelet-rich gel supernatants.
abstract: OBJECTIVE
  To compare five activation methods in equine platelet-rich plasma (PRP) by determination of platelet-derived growth factor BB (PDGF-BB) and transforming growth factor beta 1 (TGF-β1) concentrations in platelet-rich gel (PRG) supernatants.
  
  
  METHODS
  Platelet-rich plasma from 20 horses was activated by calcium chloride (CC), calcium gluconate (CG), bovine thrombin (BT), and their combinations, BTCC and BTCG. Both growth factor concentrations in PRG supernatants were measured by ELISA and compared with plasma and platelet lysates (PL) over time.
  
  
  RESULTS
  Growth factor concentrations were significantly lower in plasma and higher for all PRG supernatants. Platelet lysates contained a significantly lower concentration of PDGF-BB than PRG supernatants and a significantly higher concentration of TGF-β1 than PRG supernatants. Clots from PRP activated with sodium salts were more stable over time and had significant growth factor release, whereas CC produced gross salt deposition. Significant correlations were noticed for platelet with leukocyte concentrations in PRP (rs: 0.76), platelet counts in PRP with TGF-β1 concentrations in PRG supernatants (rs: 0.86), platelet counts in PRP with PDGF-BB concentrations in PRG supernatants (rs: 0.78), leukocyte counts in PRP with TGF-β1 concentrations in PRG supernatants (rs: 0.76), and PDGF-BB concentrations with activating substances (rs: 0.72).
  
  
  CONCLUSIONS
  Calcium gluconate was the better substance to induce PRP activation. It induced growth factor release free from calcium precipitates in the clots. Use of BT alone or combined with calcium salts was not advantageous for growth factor release.
authors.forename: Carlos E
authors.initials: CE
authors.lastname: Giraldo
authors.forename: María E
authors.initials: ME
authors.lastname: Álvarez
authors.affiliation: Prof. Jorge U. Carmona, MVZ, MSc, PhD, Departamento de Salud Animal, Universidad de Caldas, Calle 65, No 26-10, Manizales, Colombia, Phone/Fax: +57 68 781516, E-mail: carmona@ucaldas.edu.co.
authors.forename: Jorge U
authors.initials: JU
authors.lastname: Carmona
mesh_terms: D000818:Animals; D001777:Blood Coagulation; D001792:Blood Platelets; D002118:Calcium / Q000494:pharmacology; D002417:Cattle; D005782:Gels; D006736:Horses; D007958:Leukocyte Count; D007962:Leukocytes; D008297:Male; D010976:Platelet Count; D010982:Platelet-Derived Growth Factor / Q000378:metabolism*; D053657:Platelet-Rich Plasma / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D013917:Thrombin / Q000494:pharmacology; D053773:Transforming Growth Factor beta1 / Q000378:metabolism*
chemical_list: D005782:Gels; D010982:Platelet-Derived Growth Factor; D053773:Transforming Growth Factor beta1; D013917:Thrombin; D002118:Calcium
keywords: Horse; bovine thrombin; calcium salts; equine; platelet concentrates; platelet-derived growth factor BB; transforming growth factor beta 1