title: Chromatin Immunoprecipitation of Histone Modifications in Fission Yeast.
abstract: Chromatin immunoprecipitation (ChIP) is a sensitive, accurate, and reliable technique widely used to analyze protein-DNA interactions at specific binding sites in vivo. It has been a particularly powerful technique for mapping of histone modification patterns both at individual loci and genome-wide. Here we provide a detailed protocol for ChIP of histone modifications associated with active transcription in fission yeast (Schizosaccharomyces pombe).
authors.affiliation: Department of Pharmacology and Therapeutics, McGill University, Montreal, QC, Canada.
authors.forename: Jean
authors.initials: J
authors.lastname: Mbogning
authors.affiliation: Department of Pharmacology and Therapeutics, McGill University, Montreal, QC, Canada. jason.tanny@mcgill.ca.
authors.forename: Jason C
authors.initials: JC
authors.lastname: Tanny
mesh_terms: D002843:Chromatin / Q000378:metabolism; D047369:Chromatin Immunoprecipitation / Q000379:methods*; D006657:Histones / Q000378:metabolism*; D011499:Protein Processing, Post-Translational; D012568:Schizosaccharomyces / Q000235:genetics* / Q000378:metabolism*; D029702:Schizosaccharomyces pombe Proteins / Q000378:metabolism
chemical_list: D002843:Chromatin; D006657:Histones; D029702:Schizosaccharomyces pombe Proteins
keywords: ChIP; Chromatin immunoprecipitation; Fission yeast; Histone modification; S. pombe