title: Single nucleotide polymorphisms in major histocompatibility class II haplotypes are associated with potential resistance to inflammatory bowel disease in German shepherd dogs.
abstract: German shepherd dogs (GSD) in the UK are at increased risk of developing the Inflammatory Bowel Disaese (IBD). IBD is believed to be a multifactorial immune mediated disease affecting genetically predisposed dogs. The aim of the current study was to investigate whether susceptibility to IBD in GSD is associated with the major histocompatibility complex (MHC) class II locus (Dog Leukocyte Antigen, DLA). Sequence-based genotyping of the three polymorphic DLA genes DLA-DRB1, -DQA1 and -DQB1 was performed in 56 GSDs affected by IBD and in 50 breed-matched controls without any history of gastrointestinal signs. The haplotype DLA-DRB1*015:02-DQA1*006:01-DQB1*023:01 was found to be present only in the control population and was associated with a reduced risk of IBD (P<0.001). In contrast, the haplotype DLA-DRB1*015:01-DQA1*006:01-DQB1*003:01 was associated with IBD (Odds ratio [OR]=1.93, confidence interval [CI]=1.02-3.67, P=0.05). This study has identified an association between DLA-type and canine IBD, supporting the immunogenetic aetiology and immunopathogenesis of this disease.
authors.affiliation: Department of Clinical Sciences and Services, Royal Veterinary College, University of London, North Mymms, United Kingdom. Electronic address: apeiravan@rvc.ac.uk.
authors.forename: Atiyeh
authors.initials: A
authors.lastname: Peiravan
authors.affiliation: Department of Clinical Sciences and Services, Royal Veterinary College, University of London, North Mymms, United Kingdom.
authors.forename: Karin
authors.initials: K
authors.lastname: Allenspach
authors.affiliation: Department of Pathology and Pathogen Biology, Royal Veterinary College, University of London, North Mymms, United Kingdom.
authors.forename: Alisdair M
authors.initials: AM
authors.lastname: Boag
authors.affiliation: Department of Pathology and Pathogen Biology, Royal Veterinary College, University of London, North Mymms, United Kingdom.
authors.forename: Francesca
authors.initials: F
authors.lastname: Soutter
authors.affiliation: Department of Pathology and Pathogen Biology, Royal Veterinary College, University of London, North Mymms, United Kingdom.
authors.forename: Angela
authors.initials: A
authors.lastname: Holder
authors.affiliation: Department of Pathology and Pathogen Biology, Royal Veterinary College, University of London, North Mymms, United Kingdom.
authors.forename: Brian
authors.initials: B
authors.lastname: Catchpole
authors.affiliation: Centre for Integrated Genomic Medical Research, University of Manchester, Manchester, United Kingdom.
authors.forename: Lorna J
authors.initials: LJ
authors.lastname: Kennedy
authors.affiliation: Department of Pathology and Pathogen Biology, Royal Veterinary College, University of London, North Mymms, United Kingdom.
authors.forename: Dirk
authors.initials: D
authors.lastname: Werling
authors.affiliation: Department of Clinical Sciences and Services, Royal Veterinary College, University of London, North Mymms, United Kingdom. Electronic address: Fabio@andersonmoores.com.
authors.forename: Fabio
authors.initials: F
authors.lastname: Procoli
mesh_terms: D000818:Animals; D004283:Dog Diseases / Q000235:genetics* / Q000276:immunology*; D004285:Dogs; D005260:Female; D005802:Genes, MHC Class II; D020022:Genetic Predisposition to Disease; D006239:Haplotypes; D015395:Histocompatibility Antigens Class I / Q000235:genetics; D015212:Inflammatory Bowel Diseases / Q000235:genetics / Q000276:immunology / Q000662:veterinary*; D008297:Male; D020641:Polymorphism, Single Nucleotide
chemical_list: D015395:Histocompatibility Antigens Class I; C039692:histocompatibility antigen DLA
keywords: Canine; DLA II; GSD; Inflammatory bowel disease; MHC II