title: Reducing sugar loss in enzymatic hydrolysis of ethylenediamine pretreated corn stover.
abstract: In this study, the effect of ethylenediamine (EDA) on enzymatic hydrolysis with different cellulosic substrates and the approaches to reduce sugar loss in enzymatic hydrolysis were investigated. During enzymatic hydrolysis, xylose yield reduced 21.2%, 18.1% and 13.0% with 7.5mL/L EDA for AFEX pretreated corn stover (CS), washed EDA pretreated CS and CS cellulose. FTIR and GPC analysis demonstrated EDA reacted with sugar and produced high molecular weight (MW) compounds. EDA was prone to react with xylose other than glucose. H2O2and Na2SO3cannot prevent sugar loss in glucose/xylose-EDA mixture, although they inhibited the browning and high MW compounds formation. By decreasing temperature to 30°C, the loss of xylose yield reduced to only 3.8%, 3.6% and 4.2% with 7.5mL/L EDA in the enzymatic hydrolysis of AFEX pretreated CS, washed EDA pretreated CS and CS cellulose.
authors.affiliation: Key Laboratory of Systems Bioengineering (Ministry of Education), School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, PR China; SynBio Research Platform, Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), Tianjin University, Tianjin 300072, PR China.
authors.forename: Wen-Chao
authors.initials: WC
authors.lastname: Li
authors.affiliation: Key Laboratory of Systems Bioengineering (Ministry of Education), School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, PR China; SynBio Research Platform, Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), Tianjin University, Tianjin 300072, PR China.
authors.forename: Xia
authors.initials: X
authors.lastname: Li
authors.affiliation: Key Laboratory of Systems Bioengineering (Ministry of Education), School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, PR China; SynBio Research Platform, Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), Tianjin University, Tianjin 300072, PR China.
authors.forename: Lei
authors.initials: L
authors.lastname: Qin
authors.affiliation: Key Laboratory of Systems Bioengineering (Ministry of Education), School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, PR China; SynBio Research Platform, Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), Tianjin University, Tianjin 300072, PR China.
authors.forename: Jia-Qing
authors.initials: JQ
authors.lastname: Zhu
authors.affiliation: Key Laboratory of Systems Bioengineering (Ministry of Education), School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, PR China; SynBio Research Platform, Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), Tianjin University, Tianjin 300072, PR China.
authors.forename: Xiao
authors.initials: X
authors.lastname: Han
authors.affiliation: Key Laboratory of Systems Bioengineering (Ministry of Education), School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, PR China; SynBio Research Platform, Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), Tianjin University, Tianjin 300072, PR China. Electronic address: bzli@tju.edu.cn.
authors.forename: Bing-Zhi
authors.initials: BZ
authors.lastname: Li
authors.affiliation: Key Laboratory of Systems Bioengineering (Ministry of Education), School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, PR China; SynBio Research Platform, Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), Tianjin University, Tianjin 300072, PR China.
authors.forename: Ying-Jin
authors.initials: YJ
authors.lastname: Yuan
mesh_terms: D002241:Carbohydrates / Q000737:chemistry*; D002480:Cellulase / Q000378:metabolism*; D005029:Ethylenediamines / Q000494:pharmacology*; D005947:Glucose / Q000032:analysis; D006861:Hydrogen Peroxide / Q000494:pharmacology; D006868:Hydrolysis; D015416:Maillard Reaction; D008962:Models, Theoretical; D008970:Molecular Weight; D012973:Sodium Hypochlorite / Q000494:pharmacology; D017550:Spectroscopy, Fourier Transform Infrared; D013447:Sulfites / Q000494:pharmacology; D013696:Temperature; D014866:Waste Products / Q000032:analysis*; D014994:Xylose / Q000032:analysis; D003313:Zea mays / Q000737:chemistry*
chemical_list: D002241:Carbohydrates; D005029:Ethylenediamines; D013447:Sulfites; D014866:Waste Products; C031234:ethylenediamine; D014994:Xylose; D006861:Hydrogen Peroxide; D012973:Sodium Hypochlorite; D002480:Cellulase; D005947:Glucose; C009279:sodium bisulfite
keywords: Carbohydrate; Corn stover; Enzymatic hydrolysis; Ethylenediamine pretreatment; Maillard reaction