title: Beneficial effects of nontoxic ozone on H2O2-induced stress and inflammation.
abstract: In this study, the anti-oxidant and anti-inflammatory efficacy of ozone oxidative preconditioning (OOP) were investigated on hydrogen peroxide (H2O2)-induced human lung alveolar cells. In MTT and trypan blue viability tests, while 100 μmol/L H2O2caused a 17.3% and 21.9% decrease in the number of living cells, respectively, ozone at 20 μmol/L regenerated cell proliferation and prevented 9.6% and 11.0% of cell loss, respectively. In addition, H2O2decreased the transcription levels of catalase (CAT), glutathione peroxidase (GPx), and superoxide dismutase (SOD) 5.43-, 2.89-, and 5.33-fold, respectively, while it increased Bax, NF-κβ, TNF-α, and iNOS expression 1.57-, 1.32-, 1.40-, and 1.41-fold, respectively. Ozone pretreatment, however, increased CAT, GPx, and SOD transcription levels 7.08-, 5.17-, and 6.49-fold and decreased Bax, NF-κβ, TNF-α, and iNOS transcriptions by 1.25-, 0.76-, 3.63-, and 7.91-fold, respectively. Moreover, intracellular glutathione (GSH) level and SOD activity were decreased by 46.2% and 45.0% in the H2O2treatment group, and OOP recovered 58.5% and 20.1% of the decreases caused by H2O2. H2O2also increased nitrite levels 7.84-fold, and OOP reduced this increase by half. Consequently, OOP demonstrated potent anti-oxidant and anti-inflammatory effects on in vitro model of oxidative stress-induced lung injury.
authors.affiliation: a Mustafa Kemal University, Veterinary Faculty, Department of Biochemistry, Hatay 31060, Turkey.
authors.forename: Altug
authors.initials: A
authors.lastname: Kucukgul
authors.affiliation: b Trakya University, Faculty of Medicine, Department of Medical Biology, Edirne 22050, Turkey.
authors.forename: Suat
authors.initials: S
authors.lastname: Erdogan
authors.affiliation: c Mustafa Kemal University, Veterinary Faculty, Department of Surgery, Hatay 31060, Turkey.
authors.forename: Ramazan
authors.initials: R
authors.lastname: Gonenci
authors.affiliation: d Firat University, Veterinary Faculty, Department of Biochemistry, Elazıg 23200, Turkey.
authors.forename: Gonca
authors.initials: G
authors.lastname: Ozan
mesh_terms: D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D000975:Antioxidants / Q000494:pharmacology*; D002289:Carcinoma, Non-Small-Cell Lung / Q000139:chemically induced / Q000188:drug therapy* / Q000473:pathology; D002374:Catalase / Q000378:metabolism; D049109:Cell Proliferation / Q000187:drug effects; D005979:Glutathione Peroxidase / Q000378:metabolism; D006801:Humans; D006861:Hydrogen Peroxide / Q000009:adverse effects*; D007249:Inflammation / Q000139:chemically induced / Q000473:pathology / Q000517:prevention & control*; D008175:Lung Neoplasms / Q000139:chemically induced / Q000188:drug therapy / Q000473:pathology; D016877:Oxidants / Q000009:adverse effects; D010084:Oxidation-Reduction; D018384:Oxidative Stress / Q000187:drug effects*; D010126:Ozone / Q000494:pharmacology*; D017382:Reactive Oxygen Species / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D015398:Signal Transduction; D013482:Superoxide Dismutase / Q000378:metabolism; D014407:Tumor Cells, Cultured
chemical_list: D000893:Anti-Inflammatory Agents; D000975:Antioxidants; D016877:Oxidants; D017382:Reactive Oxygen Species; D010126:Ozone; D006861:Hydrogen Peroxide; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase
keywords: cellules alvéolaires pulmonaires; inflammation; lung alveolar cells; oxidative stress; ozone oxidative preconditioning; préconditionnement oxydant à l’ozone; stress oxydant