title: Serum metabolomics analysis of patients with chikungunya and dengue mono/co-infections reveals distinct metabolite signatures in the three disease conditions.
abstract: Chikungunya and dengue are arboviral infections with overlapping clinical symptoms. A subset of chikungunya infection occurs also as co-infections with dengue, resulting in complications during diagnosis and patient management. The present study was undertaken to identify the global metabolome of patient sera infected with chikungunya as mono infections and with dengue as co-infections. Using nuclear magnetic resonance (NMR) spectroscopy, the metabolome of sera of three disease conditions, namely, chikungunya and dengue as mono-infections and when co-infected were ascertained and compared with healthy individuals. Further, the cohorts were analyzed on the basis of age, onset of fever and joint involvement. Here we show that many metabolites in the serum are significantly differentially regulated during chikungunya mono-infection as well as during chikungunya co-infection with dengue. We observed that glycine, serine, threonine, galactose and pyrimidine metabolisms are the most perturbed pathways in both mono and co-infection conditions. The affected pathways in our study correlate well with the clinical manifestation like fever, inflammation, energy deprivation and joint pain during the infections. These results may serve as a starting point for validations and identification of distinct biomolecules that could be exploited as biomarker candidates thereby helping in better patient management.
authors.affiliation: Vector Borne Disease group, International Centre for Genetic Engineering and Biotechnology (ICGEB), Aruna Asaf Ali Marg, New Delhi, 110067, India.
authors.forename: Jatin
authors.initials: J
authors.lastname: Shrinet
authors.affiliation: T. N. Medical College &B. Y. L. Nair Charitable Hospital, Dr. A. L. Nair Road, Mumbai, 400008, India.
authors.forename: Jayanthi S
authors.initials: JS
authors.lastname: Shastri
authors.affiliation: Vardhman Mahavir Medical College-Safdarjung Hospital, New Delhi, 110029, India.
authors.forename: Rajni
authors.initials: R
authors.lastname: Gaind
authors.affiliation: Transcription Regulation group, International Centre for Genetic Engineering and Biotechnology (ICGEB), Aruna Asaf Ali Marg, New Delhi, 110067, India.
authors.forename: Neel Sarovar
authors.initials: NS
authors.lastname: Bhavesh
authors.affiliation: Vector Borne Disease group, International Centre for Genetic Engineering and Biotechnology (ICGEB), Aruna Asaf Ali Marg, New Delhi, 110067, India.
authors.forename: Sujatha
authors.initials: S
authors.lastname: Sunil
mesh_terms: D000293:Adolescent; D000328:Adult; D017677:Age Distribution; D000368:Aged; D001798:Blood Proteins / Q000378:metabolism*; D016022:Case-Control Studies; D065632:Chikungunya Fever / Q000097:blood / Q000378:metabolism*; D002648:Child; D060085:Coinfection / Q000097:blood / Q000378:metabolism*; D003715:Dengue / Q000097:blood / Q000378:metabolism*; D005260:Female; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D053858:Metabolic Networks and Pathways; D055432:Metabolomics / Q000379:methods*; D008875:Middle Aged; D055815:Young Adult
chemical_list: D001798:Blood Proteins