title: DPPH·-luminol chemiluminescence system and its application in the determination of scutellarin in pharmaceutical injections and rat plasma with flow injection analysis.
abstract: In this article, a DPPH·-luminol chemiluminescence (CL) system was reported and the CL mechanism was discussed according to the CL kinetic properties after sequence injecting DPPH· into the DPPH·-luminol reaction mixture. It was observed that scutellarin could inhibit the CL response of the DPPH·-luminol system. Based on this observation, a simple and rapid flow injection CL method was developed for the determination of scutellarin using the inhibition effect in alkaline medium. The optimized chemical conditions for the CL reaction were 5 × 10-6mol/L DPPH· and 1.0 × 10-4mol/L luminol in 0.01 mol/L NaOH. Under optimized conditions, the CL intensity was inversely proportional to the concentration of scutellarin over the ranges 5-2000 and 40-3200 ng/ml in pharmaceutical injection and rat plasma, respectively. The limits of detection (S/N = 3) were 5 and 40 ng/ml in preparations and rat plasma, respectively. Furthermore, the precision, recovery and stability of the validated method were acceptable for the determination of scutellarin in both pharmaceutical injections and rat plasma. The presented method was successfully applied in the determination of scutellarin in pharmaceutical injections and real rat plasma samples.
authors.affiliation: Department of Pharmaceutical Analysis, Fujian Medical University, Fuzhou, China.|Institute of Analytical Sciences, Xi'an Jiaotong University, Xi'an, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Yao
authors.affiliation: Department of Pharmaceutical Analysis, Fujian Medical University, Fuzhou, China.
authors.forename: Xiaomei
authors.initials: X
authors.lastname: Huang
authors.affiliation: Department of Traditional Chinese Medicine Resource and Bee Products, Fujian Agriculture and Forestry University, Fuzhou, China.
authors.forename: Peiying
authors.initials: P
authors.lastname: Shi
authors.affiliation: Department of Pharmaceutical Analysis, Fujian Medical University, Fuzhou, China.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Lin
authors.affiliation: Department of Pharmaceutical Analysis, Fujian Medical University, Fuzhou, China.
authors.forename: Meilan
authors.initials: M
authors.lastname: Zhu
authors.affiliation: Department of Pharmaceutical Analysis, Fujian Medical University, Fuzhou, China.
authors.forename: Ailin
authors.initials: A
authors.lastname: Liu
authors.affiliation: Department of Pharmaceutical Analysis, Fujian Medical University, Fuzhou, China.
authors.forename: Xinhua
authors.initials: X
authors.lastname: Lin
authors.affiliation: Institute of Analytical Sciences, Xi'an Jiaotong University, Xi'an, China.
authors.forename: Yuhai
authors.initials: Y
authors.lastname: Tang
mesh_terms: D000818:Animals; D047310:Apigenin / Q000032:analysis* / Q000097:blood; D001713:Biphenyl Compounds / Q000737:chemistry*; D017022:Flow Injection Analysis / Q000379:methods*; D005965:Glucuronates / Q000032:analysis* / Q000097:blood; D057230:Limit of Detection; D049408:Luminescent Agents / Q000737:chemistry; D008163:Luminescent Measurements / Q000379:methods; D008165:Luminol / Q000737:chemistry*; D010851:Picrates / Q000737:chemistry*; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results
chemical_list: D001713:Biphenyl Compounds; D005965:Glucuronates; D049408:Luminescent Agents; D010851:Picrates; C484876:scutellarin; D008165:Luminol; D047310:Apigenin; C004931:1,1-diphenyl-2-picrylhydrazyl
keywords: DPPH; chemiluminescence; flow injection; pharmaceutical injections; rat plasma; scutellarin