title: RNASET2 silencing affects miRNAs and target gene expression pattern in a human ovarian cancer cell model.
abstract: Ribonucleases (RNases) are hydrolytic enzymes endowed with the ability to either process or degrade ribonucleic acids. Among the many biological functions assigned to RNases, a growing attention has been recently devoted to the control of cancer growth, in the attempt to bring novel therapeutic approaches to clinical oncology. Indeed, several enzymes belonging to different ribonuclease families have been reported in the last decade to display a marked oncosuppressive activity in a wide range of experimental models. The human RNASET2 gene, the only member of the highly conserved T2/Rh/S family of endoribonucleolytic enzymes described in our species, has been shown to display oncosuppressive roles in both in vitro and in vivo models representing several human malignancies. In the present study, we extend previous findings obtained in ovarian cancer models to shed further light on the cell-autonomous roles played by this gene in the context of its oncosuppresive role and to show that RNASET2 silencing can significantly affect the transcriptional output in one of the most thoroughly investigated human ovarian cancer cell lines. Moreover, we report for the first time that RNASET2-mediated changes in the cell transcriptome are in part mediated by its apparent ability to affect the cell's microRNA expression pattern.
authors.affiliation: Department of Theoretical and Applied Sciences, University of Insubria, Varese, Italy.
authors.forename: Giovanna
authors.initials: G
authors.lastname: Turconi
authors.affiliation: Department of Biotechnology and Life Sciences, University of Insubria, Varese, Italy.
authors.forename: Debora
authors.initials: D
authors.lastname: Scaldaferri
authors.affiliation: Department of Clinical and Experimental Medicine, University of Insubria, Varese, Italy.
authors.forename: Marco
authors.initials: M
authors.lastname: Fabbri
authors.affiliation: Department of Biotechnology and Life Sciences, University of Insubria, Varese, Italy.
authors.forename: Laura
authors.initials: L
authors.lastname: Monti
authors.affiliation: Department of Theoretical and Applied Sciences, University of Insubria, Varese, Italy.
authors.forename: Marta
authors.initials: M
authors.lastname: Lualdi
authors.affiliation: Department of Biotechnology and Life Sciences, University of Insubria, Varese, Italy.
authors.forename: Edoardo
authors.initials: E
authors.lastname: Pedrini
authors.affiliation: JRC Directorate F - Health, Consumers and Reference Materials, Chemical Safety and Alternative Methods Unit, Ispra, Varese (VA), Italy.
authors.forename: Laura
authors.initials: L
authors.lastname: Gribaldo
authors.affiliation: Department of Biotechnology and Life Sciences, University of Insubria, Varese, Italy.
authors.forename: Roberto
authors.initials: R
authors.lastname: Taramelli
authors.affiliation: Department of Biotechnology and Life Sciences, University of Insubria, Varese, Italy.
authors.forename: Francesco
authors.initials: F
authors.lastname: Acquati
mesh_terms: D048868:Adaptor Proteins, Signal Transducing; D045744:Cell Line, Tumor; D005260:Female; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics; D006801:Humans; D035683:MicroRNAs / Q000235:genetics*; D010051:Ovarian Neoplasms / Q000235:genetics* / Q000473:pathology*; D011506:Proteins / Q000235:genetics; D034622:RNA Interference; D012333:RNA, Messenger / Q000235:genetics*; D034741:RNA, Small Interfering / Q000235:genetics; D012260:Ribonucleases / Q000235:genetics*; D059467:Transcriptome / Q000235:genetics; D025521:Tumor Suppressor Proteins / Q000235:genetics*
chemical_list: D048868:Adaptor Proteins, Signal Transducing; C498040:DDIT4L protein, human; D035683:MicroRNAs; D011506:Proteins; D012333:RNA, Messenger; D034741:RNA, Small Interfering; D025521:Tumor Suppressor Proteins; D012260:Ribonucleases; C425232:RNASET2 protein, human