title: Characterization of a specific monoclonal antibody against immunoglobulin light kappa/L1 chain in olive flounder (Paralichthys olivaceus).
abstract: Immunoglobulins (Ig) are heterodimeric proteins that play critical roles in the adaptive immune system of vertebrates. Because of their plasticity, teleostean Igs are more diverse, and thus do not conform to mammalian classifications. Because of this, mammalian-based Ig cell markers cannot be used successfully to study immune responses in fish. There is therefore a need to produce Ig-specific cell markers for fish. Here, we attempted to identify the specific isotype detected by an Ig light chain-specific monoclonal antibody (anti-olive flounder IgL-mAb: M7C3-4) that we had previously produced [11]. Three newly identified sequences of the Ig light chain from olive flounder were classified according to their isotypes. Subsequent analyses revealed that M7C3-4 was able to specifically detect lymphocytes expressing one of the κ chains (Igκ-a) in olive flounder. Interestingly, Igκ-a+B cells were more abundant in spleen and trunk-kidney than in peripheral blood, indicating a distribution different from that of IgM+B cells. Our work reveals interesting aspects of B cell distribution and differentiation, and may aid in the production of suitable and effective cell markers for olive flounder.
authors.affiliation: Laboratory of Aquatic Animal Diseases, Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University, Jinju 660-701, South Korea.
authors.forename: Young Kyu
authors.initials: YK
authors.lastname: Kim
authors.affiliation: Laboratory of Aquatic Animal Diseases, Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University, Jinju 660-701, South Korea.
authors.forename: Jung Seok
authors.initials: JS
authors.lastname: Lee
authors.affiliation: Laboratory of Aquatic Animal Diseases, Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University, Jinju 660-701, South Korea.
authors.forename: Jae Wook
authors.initials: JW
authors.lastname: Jung
authors.affiliation: Department of Biochemistry and Applied Biosciences, Faculty of Agriculture, University of Miyazaki, 1-1 Gakuen-kibanadai-nishi, Miyazaki 889-2192, Japan.
authors.forename: Jun-Ichi
authors.initials: JI
authors.lastname: Hikima
authors.affiliation: Department of Veterinary Diseases Biology, Faculty of Health and Medical Science, University of Copenhagen, Copenhagen 13626, Denmark.
authors.forename: Maki
authors.initials: M
authors.lastname: Ohtani
authors.affiliation: Laboratory of Aquatic Animal Diseases, Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University, Jinju 660-701, South Korea.
authors.forename: Ho Bin
authors.initials: HB
authors.lastname: Jang
authors.affiliation: Laboratory of Aquatic Animal Diseases, Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University, Jinju 660-701, South Korea.
authors.forename: Seong Won
authors.initials: SW
authors.lastname: Nho
authors.affiliation: Laboratory of Aquatic Animal Diseases, Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University, Jinju 660-701, South Korea.
authors.forename: In Seok
authors.initials: IS
authors.lastname: Cha
authors.affiliation: Laboratory of Aquatic Animal Diseases, Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University, Jinju 660-701, South Korea.
authors.forename: Seong Bin
authors.initials: SB
authors.lastname: Park
authors.affiliation: Inland Aquaculture Research Center, National Institute of Fisheries Science (NIFS), Changwon 51688, South Korea.
authors.forename: Jeong Ho
authors.initials: JH
authors.lastname: Lee
authors.affiliation: Consolidated Research Institute for Advanced Science and Medical Care, Waseda University, 513, Wasedatsurumaki-cho, Shinjuku-ku, Tokyo 162-0041, Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Aoki
authors.affiliation: Laboratory of Aquatic Animal Diseases, Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University, Jinju 660-701, South Korea. Electronic address: jungts@gnu.ac.kr.
authors.forename: Tae Sung
authors.initials: TS
authors.lastname: Jung
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000235:genetics* / Q000378:metabolism; D001402:B-Lymphocytes / Q000378:metabolism; D029941:Fish Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D005412:Flatfishes / Q000235:genetics* / Q000276:immunology; D005434:Flow Cytometry / Q000662:veterinary; D007145:Immunoglobulin kappa-Chains / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D018613:Microscopy, Confocal / Q000662:veterinary; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000662:veterinary
chemical_list: D000911:Antibodies, Monoclonal; D029941:Fish Proteins; D007145:Immunoglobulin kappa-Chains
keywords: Igκ-a; Immunoglobulin (Ig); Monoclonal antibodies (mAbs); Olive flounder