title: Red blood cell phase separation in symmetric and asymmetric microchannel networks: effect of capillary dilation and inflow velocity.
abstract: The non-uniform partitioning or phase separation of red blood cells (RBCs) at a diverging bifurcation of a microvascular network is responsible for RBC heterogeneity within the network. The mechanisms controlling RBC heterogeneity are not yet fully understood and there is a need to improve the basic understanding of the phase separation phenomenon. In this context, in vitro experiments can fill the gap between existing in vivo and in silico models as they provide better controllability than in vivo experiments without mathematical idealizations or simplifications inherent to in silico models. In this study, we fabricated simple models of symmetric/asymmetric microvascular networks; we provided quantitative data on the RBC velocity, line density and flux in the daughter branches. In general our results confirmed the tendency of RBCs to enter the daughter branch with higher flow rate (Zweifach-Fung effect); in some cases even inversion of the Zweifach-Fung effect was observed. We showed for the first time a reduction of the Zweifach-Fung effect with increasing flow rate. Moreover capillary dilation was shown to cause an increase of RBC line density and RBC residence time within the dilated capillary underlining the possible role of pericytes in regulating the oxygen supply.
authors.affiliation: ARTORG Center, University of Bern, Bern, Switzerland.
authors.forename: Francesco
authors.initials: F
authors.lastname: Clavica
authors.affiliation: Haute Ecole Arc Ingénierie, HES-SO // University of Applied Sciences Western Switzerland, La Chaux-de-Fonds, Switzerland.
authors.forename: Alexandra
authors.initials: A
authors.lastname: Homsy
authors.affiliation: Haute Ecole Arc Ingénierie, HES-SO // University of Applied Sciences Western Switzerland, La Chaux-de-Fonds, Switzerland.
authors.forename: Laure
authors.initials: L
authors.lastname: Jeandupeux
authors.affiliation: ARTORG Center, University of Bern, Bern, Switzerland.
authors.forename: Dominik
authors.initials: D
authors.lastname: Obrist
mesh_terms: D000818:Animals; D002196:Capillaries / Q000166:cytology / Q000502:physiology; D003198:Computer Simulation; D004912:Erythrocytes / Q000502:physiology*; D056656:Lab-On-A-Chip Devices; D008833:Microcirculation; D055806:Microvessels / Q000166:cytology / Q000502:physiology; D008955:Models, Cardiovascular; D034421:Sus scrofa; D014664:Vasodilation