title: Global profiling of cellular targets of gambogic acid by quantitative chemical proteomics.
abstract: Gambogic acid has shown significant potential as an anti-cancer and anti-inflammatory compound; however, the molecular mechanism underlying the actions of gambogic acid, particularly its cellular target profiles, has not been investigated. In this study, we report the proteome-wide profiling of potential cellular targets of gambogic acid using quantitative mass spectrometry-based chemical proteomics. The target profiles of gambogic acid in living HeLa and K562 cells were identified, which strengthened our understanding of the mode of actions of the phytochemical.
authors.affiliation: CAS Key Laboratory of Synthetic Biology, CAS Centre for Excellence in Molecular Plant Sciences, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200032, China. ylxiao@sibs.ac.cn.
authors.forename: Yiqing
authors.identifier: http://orcid.org/0000-0002-6391-3259
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: CAS Key Laboratory of Synthetic Biology, CAS Centre for Excellence in Molecular Plant Sciences, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200032, China. ylxiao@sibs.ac.cn and University of Chinese Academy of Sciences, Beijing 100039, China.
authors.forename: Weichao
authors.initials: W
authors.lastname: Li
authors.affiliation: University of Chinese Academy of Sciences, Beijing 100039, China and Institute for Nutritional Sciences, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200032, China.
authors.forename: Xixi
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Institute for Nutritional Sciences, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200032, China.
authors.forename: Haibing
authors.initials: H
authors.lastname: Zhang
authors.affiliation: CAS Key Laboratory of Synthetic Biology, CAS Centre for Excellence in Molecular Plant Sciences, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200032, China. ylxiao@sibs.ac.cn and University of Chinese Academy of Sciences, Beijing 100039, China.
authors.forename: Youli
authors.initials: Y
authors.lastname: Xiao
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology*; D006367:HeLa Cells; D006801:Humans; D020014:K562 Cells; D013058:Mass Spectrometry; D058990:Molecular Targeted Therapy; D040901:Proteomics; D044004:Xanthones / Q000494:pharmacology*
chemical_list: D000970:Antineoplastic Agents; D044004:Xanthones; C052659:gambogic acid