title: Carbon-based nanocomposites with aptamer-templated silver nanoclusters for the highly sensitive and selective detection of platelet-derived growth factor.
abstract: We synthesized two kinds of carbon-based nanocomposites of silver nanoclusters (AgNCs). An aptamer for targeted platelet-derived growth factor-BB (PDGF-BB) detection was used as the organic phase to produce AgNCs@Apt, three dimensional reduced graphene oxide@AgNCs@Aptamer (3D-rGO@AgNCs@Apt), and graphene quantum dots@AgNCs@Aptamer (GQD@AgNCs@Apt) nanocomposites. The formation mechanism of the developed nanocomposites was described by detailed characterizations of their chemical and crystal structures. Subsequently, the as-synthesized nanoclusters containing aptamer strands were applied as the sensitive layers to fabricate a novel electrochemical aptasensor for the detection of PDGF-BB, which may be directly used to determine the target protein. Electrochemical impedance spectra showed that the developed 3D-rGO@AgNCs@Apt-based biosensor exhibited the highest sensitivity for PDGF-BB detection among three kinds of fabricated aptasensors, with an extremely low detection limit of 0.82pgmL-1. In addition, the 3D-rGO@AgNCs@Apt-based biosensor showed high selectivity, stability, and applicability for the detection of PDGF-BB. This finding indicated that the AgNC-based nanocomposites prepared by a one-step method could be used as an electrochemical biosensor for various detection procedures in the biomedical field.
authors.affiliation: Henan Provincial Key Laboratory of Surface and Interface Science, Zhengzhou University of Light Industry, No. 136, Science Avenue, Zhengzhou 450001, China. Electronic address: mainzhh@163.com.
authors.forename: Zhihong
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: Henan Provincial Key Laboratory of Surface and Interface Science, Zhengzhou University of Light Industry, No. 136, Science Avenue, Zhengzhou 450001, China.
authors.forename: Chuanpan
authors.initials: C
authors.lastname: Guo
authors.affiliation: Henan Provincial Key Laboratory of Surface and Interface Science, Zhengzhou University of Light Industry, No. 136, Science Avenue, Zhengzhou 450001, China.
authors.forename: Shuai
authors.initials: S
authors.lastname: Zhang
authors.affiliation: Henan Provincial Key Laboratory of Surface and Interface Science, Zhengzhou University of Light Industry, No. 136, Science Avenue, Zhengzhou 450001, China.
authors.forename: Linghao
authors.initials: L
authors.lastname: He
authors.affiliation: Henan Provincial Key Laboratory of Surface and Interface Science, Zhengzhou University of Light Industry, No. 136, Science Avenue, Zhengzhou 450001, China.
authors.forename: Minghua
authors.initials: M
authors.lastname: Wang
authors.affiliation: Henan Provincial Key Laboratory of Surface and Interface Science, Zhengzhou University of Light Industry, No. 136, Science Avenue, Zhengzhou 450001, China.
authors.forename: Donglai
authors.initials: D
authors.lastname: Peng
authors.affiliation: Henan Provincial Key Laboratory of Surface and Interface Science, Zhengzhou University of Light Industry, No. 136, Science Avenue, Zhengzhou 450001, China.
authors.forename: Junfeng
authors.initials: J
authors.lastname: Tian
authors.affiliation: Henan Provincial Key Laboratory of Surface and Interface Science, Zhengzhou University of Light Industry, No. 136, Science Avenue, Zhengzhou 450001, China.
authors.forename: Shaoming
authors.initials: S
authors.lastname: Fang
mesh_terms: D052157:Aptamers, Nucleotide / Q000737:chemistry*; D000077214:Becaplermin; D015374:Biosensing Techniques / Q000379:methods*; D002244:Carbon / Q000737:chemistry; D058266:Dielectric Spectroscopy / Q000379:methods; D006108:Graphite / Q000737:chemistry*; D006801:Humans; D057230:Limit of Detection; D053761:Nanocomposites / Q000737:chemistry* / Q000648:ultrastructure; D010087:Oxides / Q000737:chemistry; D020574:Proto-Oncogene Proteins c-sis / Q000097:blood*; D012834:Silver / Q000737:chemistry*
chemical_list: D052157:Aptamers, Nucleotide; D010087:Oxides; D020574:Proto-Oncogene Proteins c-sis; D000077214:Becaplermin; D012834:Silver; D002244:Carbon; D006108:Graphite
keywords: Carbon-based nanocomposites; Electrochemical biosensor; Electrochemical impedance spectra; PDGF-BB; Silver nanoclusters