title: Hyperthermic carbon dioxide pneumoperitoneum reinforces the inhibition of 5-FU on the proliferation and invasion of colon cancer.
abstract: The effect of hyperthermic carbon dioxide (CO2) pneumoperitoneum in combination with 5-fluorouracil (5-FU) on the proliferation and invasion of colon cancer was explored. Colon cancer cell line SW-480 was sealed into the urine collection bag to simulate pneumoperitoneum with 100% CO2 under a pressure of 12 mmHg. The cells were divided into group A, CO2 at 37˚C; group B, CO2 at 43˚C; group C, 5-FU; group D, CO2 at 37˚C+5-FU; group E, CO2 at 43˚C+5-FU; and control groups under normal culture conditions. The cell proliferation was assessed by CCK-8 test; the cell apoptosis was tested by FACS analysis; the cell invasion was examined by Transwell assay; the expression of HSP-70, caspase-3, HIF-1α and MMP-9 proteins and genes were detected by western blot analysis and RT-PCR. The SW-480 cells were injected into nude mouse cecum subserosal to establish a colon cancer model. We applied 43˚C CO2 pneumoperitoneum or 5-FU intraperitoneal chemotherapy to intervene, detected the transplantation tumor growth and metastasis. The cell proliferation was inhibited in groups B, C, D and E, apoptosis was induced in groups B, C, D and E, the Transwell cell number decreased in groups B, C, D and E, the transplantation tumor weight and metastasis rate were inhibited in groups B, C, D and E, but all not in group A. The most significant change was observed in group E. Hyperthermic CO2 pneumoperitoneum was able to reinforce the inhibition of 5-FU on proliferation and invasion of colon cancer.
authors.affiliation: Department of General Surgery, The Fifth People's Hospital of Shanghai, Fudan University, Shanghai 200240, P.R. China.
authors.forename: Jiaying
authors.initials: J
authors.lastname: Zhao
authors.affiliation: Department of General Surgery, The Fifth People's Hospital of Shanghai, Fudan University, Shanghai 200240, P.R. China.
authors.forename: You
authors.initials: Y
authors.lastname: Lv
authors.affiliation: Department of General Surgery, The Fifth People's Hospital of Shanghai, Fudan University, Shanghai 200240, P.R. China.
authors.forename: Yuankun
authors.initials: Y
authors.lastname: Cai
authors.affiliation: Life Science Research Institute, Chinese Academy of Sciences, Shanghai 200031, P.R. China.
authors.forename: Wangui
authors.initials: W
authors.lastname: Wei
authors.affiliation: Department of General Surgery, The Fifth People's Hospital of Shanghai, Fudan University, Shanghai 200240, P.R. China.
authors.forename: Chenqing
authors.initials: C
authors.lastname: Yin
authors.affiliation: Department of General Surgery, The Fifth People's Hospital of Shanghai, Fudan University, Shanghai 200240, P.R. China.
authors.forename: Xin
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of General Surgery, The Fifth People's Hospital of Shanghai, Fudan University, Shanghai 200240, P.R. China.
authors.forename: Zong
authors.initials: Z
authors.lastname: Hao
authors.affiliation: Department of General Surgery, The Fifth People's Hospital of Shanghai, Fudan University, Shanghai 200240, P.R. China.
authors.forename: Chenxia
authors.initials: C
authors.lastname: Shen
authors.affiliation: Department of General Surgery, The Fifth People's Hospital of Shanghai, Fudan University, Shanghai 200240, P.R. China.
authors.forename: Huipeng
authors.initials: H
authors.lastname: Wang
mesh_terms: D000818:Animals; D002245:Carbon Dioxide / Q000008:administration & dosage*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D003110:Colonic Neoplasms / Q000473:pathology / Q000628:therapy*; D003131:Combined Modality Therapy; D005472:Fluorouracil / Q000627:therapeutic use*; D006801:Humans; D006979:Hyperthermia, Induced / Q000379:methods*; D007274:Injections, Intraperitoneal; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009361:Neoplasm Invasiveness; D011028:Pneumoperitoneum, Artificial / Q000379:methods*; D023041:Xenograft Model Antitumor Assays
chemical_list: D002245:Carbon Dioxide; D005472:Fluorouracil