title: Rubber particle proteins REF1 and SRPP1 interact differently with native lipids extracted from Hevea brasiliensis latex.
abstract: Rubber particle membranes from the Hevea latex contain predominantly two proteins, REF1 and SRPP1 involved in poly(cis-1,4-isoprene) synthesis or rubber quality. The repartition of both proteins on the small or large rubber particles seems to differ, but their role in the irreversible coagulation of the rubber particle is still unknown. In this study we highlighted the different modes of interactions of both recombinant proteins with different classes of lipids extracted from Hevea brasiliensis latex, and defined as phospholipids (PL), glycolipids (GL) and neutral lipids (NL). We combined two biophysical methods, polarization modulated-infrared reflection adsorption spectroscopy (PM-IRRAS) and ellipsometry to elucidate their interactions with monolayers of each class of lipids. REF1 and SRPP1 interactions with native lipids are clearly different; SRPP1 interacts mostly in surface with PL, GL or NL, without modification of its structure. In contrast REF1 inserts deeply in the lipid monolayers with all lipid classes. With NL, REF1 is even able to switch from α-helice conformation to β-sheet structure, as in its aggregated form (amyloid form). Interaction between REF1 and NL may therefore have a specific role in the irreversible coagulation of rubber particles.
authors.affiliation: Department of Biotechnology, Faculty of Agro-Industry, Kasetsart University, 10900 Bangkok, Thailand.
authors.forename: Kanthida
authors.initials: K
authors.lastname: Wadeesirisak
authors.affiliation: CNRS, CBMN, UMR 5248, Univ. Bordeaux, Bordeaux INP, 33600 Pessac, France.
authors.forename: Sabine
authors.initials: S
authors.lastname: Castano
authors.affiliation: CNRS, CBMN, UMR 5248, Univ. Bordeaux, Bordeaux INP, 33600 Pessac, France; CNRS, LCPO, UMR 5629, Univ. Bordeaux, Bordeaux INP, 33600 Pessac, France.
authors.forename: Karine
authors.initials: K
authors.lastname: Berthelot
authors.affiliation: CIRAD-INRA-SupAgro-Univ. Montpellier 2, UMR 1208 IATE, 34060 Montpellier, France.
authors.forename: Laurent
authors.initials: L
authors.lastname: Vaysse
authors.affiliation: CIRAD-INRA-SupAgro-Univ. Montpellier 2, UMR 1208 IATE, 34060 Montpellier, France.
authors.forename: Frédéric
authors.initials: F
authors.lastname: Bonfils
authors.affiliation: CNRS, LCPO, UMR 5629, Univ. Bordeaux, Bordeaux INP, 33600 Pessac, France.
authors.forename: Frédéric
authors.initials: F
authors.lastname: Peruch
authors.affiliation: Department of Biotechnology, Faculty of Agro-Industry, Kasetsart University, 10900 Bangkok, Thailand.
authors.forename: Kittipong
authors.initials: K
authors.lastname: Rattanaporn
authors.affiliation: Kasetsart Agricultural and Agro-Industrial Product Improvement Institute, Kasetsart University, 10900 Bangkok, Thailand.
authors.forename: Siriluck
authors.initials: S
authors.lastname: Liengprayoon
authors.affiliation: CNRS, CBMN, UMR 5248, Univ. Bordeaux, Bordeaux INP, 33600 Pessac, France. Electronic address: sophie.lecomte@cbmn.u-bordeaux.fr.
authors.forename: Sophie
authors.initials: S
authors.lastname: Lecomte
authors.affiliation: CIRAD-INRA-SupAgro-Univ. Montpellier 2, UMR 1208 IATE, 34060 Montpellier, France. Electronic address: celine.bottier@cirad.fr.
authors.forename: Céline
authors.initials: C
authors.lastname: Bottier
mesh_terms: D006017:Glycolipids / Q000378:metabolism; D045782:Hemiterpenes / Q000378:metabolism; D028482:Hevea / Q000378:metabolism*; D007840:Latex / Q000378:metabolism*; D008565:Membrane Proteins / Q000378:metabolism*; D010743:Phospholipids / Q000378:metabolism; D010940:Plant Proteins / Q000378:metabolism*; D000072756:Protein Conformation, alpha-Helical; D000072757:Protein Conformation, beta-Strand; D011994:Recombinant Proteins / Q000378:metabolism; D012408:Rubber / Q000378:metabolism*
chemical_list: D006017:Glycolipids; D045782:Hemiterpenes; D007840:Latex; D008565:Membrane Proteins; D010743:Phospholipids; D010940:Plant Proteins; D011994:Recombinant Proteins; C455100:1,4-polyisoprene; D012408:Rubber
keywords: Hevea brasiliensis rubber particle proteins; Langmuir monolayer; Latex native lipids; Polarization modulated-infrared reflection absorption spectroscopy; Rubber elongation factor; Small rubber particle protein