title: Serum apolipoprotein-A1 as a possible biomarker for monitoring treatment of canine leishmaniosis.
abstract: The aims of this study were: the identification of proteins differentially represented in the serum proteome of dogs with leishmaniosis after treatment and the verification of one selected protein as a possible biomarker for treatment monitoring. Serum samples from five dogs with leishmaniosis, before and after treatment were pooled into two groups and analysed using 2-dimensional electrophoresis followed by mass spectrometry analysis (MS). The MS analysis allowed the identification of 8 proteins differently expressed. APO-A1 was selected and an immunoturbidimetric assay was validated for its measurement in dogs. Significantly decreased concentrations of APO-A1 in dogs with leishmaniosis and a significant increase after a good response to the treatment were observed, suggesting that APO-A1 could be a potential biomarker of treatment monitoring with the advantages of an automated measurement.
authors.affiliation: Interlab-UMU, Regional Campus of International Excellence "Mare Nostrum", University of Murcia, Espinardo, Murcia 30100, Spain.
authors.forename: Damián
authors.initials: D
authors.lastname: Escribano
authors.affiliation: Interlab-UMU, Regional Campus of International Excellence "Mare Nostrum", University of Murcia, Espinardo, Murcia 30100, Spain; Department of Animal Medicine and Surgery, Veterinary School, Autonomous University of Barcelona, Barcelona, Spain.
authors.forename: Asta
authors.initials: A
authors.lastname: Tvarijonaviciute
authors.affiliation: Department of Internal Medicine, Faculty of Veterinary Medicine, Uludag University, 16059 Bursa, Turkey.
authors.forename: Meric
authors.initials: M
authors.lastname: Kocaturk
authors.affiliation: Interlab-UMU, Regional Campus of International Excellence "Mare Nostrum", University of Murcia, Espinardo, Murcia 30100, Spain.
authors.forename: Jose Joaquín
authors.initials: JJ
authors.lastname: Cerón
authors.affiliation: Interlab-UMU, Regional Campus of International Excellence "Mare Nostrum", University of Murcia, Espinardo, Murcia 30100, Spain.
authors.forename: Luis
authors.initials: L
authors.lastname: Pardo-Marín
authors.affiliation: Research Support Service, Regional Campus of International Excellence "Mare Nostrum", University of Murcia, Murcia, Spain.
authors.forename: Alejandro
authors.initials: A
authors.lastname: Torrecillas
authors.affiliation: Department of Internal Medicine, Faculty of Veterinary Medicine, Uludag University, 16059 Bursa, Turkey.
authors.forename: Zeki
authors.initials: Z
authors.lastname: Yilmaz
authors.affiliation: Interlab-UMU, Regional Campus of International Excellence "Mare Nostrum", University of Murcia, Espinardo, Murcia 30100, Spain. Electronic address: silviams@um.es.
authors.forename: Silvia
authors.initials: S
authors.lastname: Martínez-Subiela
mesh_terms: D000493:Allopurinol / Q000627:therapeutic use; D000818:Animals; D000981:Antiprotozoal Agents / Q000627:therapeutic use*; D016632:Apolipoprotein A-I / Q000097:blood*; D015415:Biomarkers / Q000097:blood*; D004285:Dogs; D015180:Electrophoresis, Gel, Two-Dimensional; D007896:Leishmaniasis / Q000097:blood / Q000175:diagnosis / Q000188:drug therapy*; D013058:Mass Spectrometry; D008536:Meglumine / Q000627:therapeutic use; D000077485:Meglumine Antimoniate; D009942:Organometallic Compounds / Q000627:therapeutic use; D020543:Proteome / Q000737:chemistry
chemical_list: D000981:Antiprotozoal Agents; D016632:Apolipoprotein A-I; D015415:Biomarkers; D009942:Organometallic Compounds; D020543:Proteome; D000493:Allopurinol; D008536:Meglumine; D000077485:Meglumine Antimoniate
keywords: Apolipoprotein A1; Dogs; Leishmaniosis; Treatment monitoring