title: Facile synthesis of Fe3O4/g-C3N4/HKUST-1 composites as a novel biosensor platform for ochratoxin A.
abstract: A fluorescent biosensor for ochratoxin A was fabricated on the basis of a new nanocomposite (Fe3O4/g-C3N4/HKUST-1 composites). Fe3O4/g-C3N4/HKUST-1 was synthesized in this work for the first time, which combined HKUST-1 with g-C3N4to improve its chemical stability. Fe3O4/g-C3N4/HKUST-1 composites have strong adsorption capacity for dye-labeled aptamer and are able to completely quench the fluorescence of the dye through the photoinduced electron transfer (PET) mechanism. In the presence of ochratoxin A (OTA), it can bind with the aptamer with high affinity, causing the releasing of the dye-labeled aptamer from the Fe3O4/g-C3N4/HKUST-1 and therefore results in the recovery of fluorescence. The fluorescence intensity of the biosensor has a linear relationship with the OTA concentration in the range of 5.0-160.0ng/mL. The LOD of sensor is 2.57ng/mL (S/N=3). This fluorescence sensor based on the Fe3O4/g-C3N4/HKUST-1 composites has been applied to detect OTA in corn with satisfying results.
authors.affiliation: Ministry of Education Key Laboratory of Analysis and Detection for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection for Food Safety, Fuzhou University, Fuzhou, Fujian 350108, PR China.
authors.forename: Shuisheng
authors.initials: S
authors.lastname: Hu
authors.affiliation: Ministry of Education Key Laboratory of Analysis and Detection for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection for Food Safety, Fuzhou University, Fuzhou, Fujian 350108, PR China.
authors.forename: Wenjun
authors.initials: W
authors.lastname: Ouyang
authors.affiliation: Ministry of Education Key Laboratory of Analysis and Detection for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection for Food Safety, Fuzhou University, Fuzhou, Fujian 350108, PR China.
authors.forename: Longhua
authors.initials: L
authors.lastname: Guo
authors.affiliation: Ministry of Education Key Laboratory of Analysis and Detection for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection for Food Safety, Fuzhou University, Fuzhou, Fujian 350108, PR China; State Key Laboratory of Photocatalysis on Energy and Environment, College of Chemistry, Fuzhou University, Fuzhou 350002, PR China.
authors.forename: Zhenyu
authors.initials: Z
authors.lastname: Lin
authors.affiliation: School of Applied Chemistry and Biological Technology, Shenzhen Polytechnic, Shenzhen, Guangdong 518055, PR China. Electronic address: remotexh@ustc.edu.cn.
authors.forename: Xiaohua
authors.initials: X
authors.lastname: Jiang
authors.affiliation: Ministry of Education Key Laboratory of Analysis and Detection for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection for Food Safety, Fuzhou University, Fuzhou, Fujian 350108, PR China. Electronic address: summer328cn@163.com.
authors.forename: Bin
authors.initials: B
authors.lastname: Qiu
authors.affiliation: Ministry of Education Key Laboratory of Analysis and Detection for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection for Food Safety, Fuzhou University, Fuzhou, Fujian 350108, PR China.
authors.forename: Guonan
authors.initials: G
authors.lastname: Chen
mesh_terms: D052157:Aptamers, Nucleotide / Q000097:blood*; D015374:Biosensing Techniques / Q000379:methods*; D052203:Ferrosoferric Oxide / Q000138:chemical synthesis / Q000737:chemistry*; D005506:Food Contamination / Q000032:analysis*; D006108:Graphite / Q000138:chemical synthesis / Q000737:chemistry; D057230:Limit of Detection; D000073396:Metal-Organic Frameworks; D049329:Nanostructures / Q000737:chemistry / Q000648:ultrastructure; D009570:Nitriles / Q000138:chemical synthesis / Q000737:chemistry*; D009793:Ochratoxins / Q000032:analysis*; D009942:Organometallic Compounds / Q000138:chemical synthesis / Q000737:chemistry*; D013050:Spectrometry, Fluorescence / Q000379:methods; D003313:Zea mays / Q000737:chemistry
chemical_list: D052157:Aptamers, Nucleotide; D000073396:Metal-Organic Frameworks; D009570:Nitriles; D009793:Ochratoxins; D009942:Organometallic Compounds; C539834:bis(1,3,5-benzenetricarboxylate)tricopper(II); C025589:ochratoxin A; C011206:cyanogen; D006108:Graphite; D052203:Ferrosoferric Oxide
keywords: Aptamer; Fe(3)O(4)/g-C(3)N(4)/HKUST-1 composites; Fluorescent biosensor; Ochratoxin A