title: Cloning and characterization of fatty acid-binding proteins (fabps) from Japanese seabass (Lateolabraxjaponicus) liver, and their gene expressions in response to dietary arachidonic acid (ARA).
abstract: In the present study, putative cDNA of five fabp isoforms, i.e., fabp1, fabp2, fabp3, fabp4, and fabp7, was cloned and characterized from the liver of Japanese seabass (Lateolabrax japonicus), and their expression in response to diets with different arachidonic acid (ARA) levels (0.05%, 0.22%, 0.37%, 0.60%, 1.38% and 2.32% of dry matter) was investigated following a feeding trial. The Japanese seabass fabps showed high identity to their orthologs in other fish species and mammals. However, a specific fabp of Japanese seabass showed much lower identity to other Japanese seabass fabps. fabp1 has high expressions in liver and intestine, whereas fabp2 is mainly expressed in the gastrointestinal tract. The highest expression level of fabp3, fabp4, and fabp7 was observed in muscle, eye, and liver respectively. Different tissue expression patterns of fabp2, fabp4, and fabp7 between Japanese seabass and other teleost may indicate specific evolutionary Fabp functions in Japanese seabass. Moderate levels of dietary ARA (0.37-0.60%) enhanced the gene expressions of fabp1 in liver and intestine, fabp2 in intestine, and fabp3 in intestine, whereas excess dietary ARA levels (1.38-2.323%) were ineffective. The highest level of dietary ARA (2.32%) increased only the expression of fabp3 in muscle compared to the control diet. Gene expressions of fabp3 and fabp7 in liver, and fabp4 in liver, intestine, and muscle were not significantly influenced by dietary ARA. To our knowledge, this is the first study investigating the regulation of fabp expressions by dietary ARA.
authors.affiliation: Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, 106 Nanjing Road, Qingdao, Shandong 266071, China; Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, 1 Wenhai Road, Qingdao, Shandong 266237, China.
authors.forename: Houguo
authors.initials: H
authors.lastname: Xu
authors.affiliation: Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, 106 Nanjing Road, Qingdao, Shandong 266071, China; Wuxi Fisheries College, Nanjing Agricultural University, Wuxi, Jiangsu 214081, China.
authors.forename: Yuanqin
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, 106 Nanjing Road, Qingdao, Shandong 266071, China.
authors.forename: Chengqiang
authors.initials: C
authors.lastname: Wang
authors.affiliation: Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, 106 Nanjing Road, Qingdao, Shandong 266071, China.
authors.forename: Yuliang
authors.initials: Y
authors.lastname: Wei
authors.affiliation: Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, 106 Nanjing Road, Qingdao, Shandong 266071, China; Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, 1 Wenhai Road, Qingdao, Shandong 266237, China.
authors.forename: Keke
authors.initials: K
authors.lastname: Zheng
authors.affiliation: Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, 106 Nanjing Road, Qingdao, Shandong 266071, China; Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, 1 Wenhai Road, Qingdao, Shandong 266237, China. Electronic address: liangmq@ysfri.ac.cn.
authors.forename: Mengqing
authors.initials: M
authors.lastname: Liang
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D016718:Arachidonic Acid / Q000494:pharmacology*; D003001:Cloning, Molecular; D004041:Dietary Fats / Q000494:pharmacology*; D050556:Fatty Acid-Binding Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D005786:Gene Expression Regulation / Q000187:drug effects*; D008099:Liver / Q000187:drug effects* / Q000378:metabolism*; D010473:Perciformes / Q000235:genetics*; D010802:Phylogeny; D021381:Protein Transport / Q000187:drug effects; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment
chemical_list: D004041:Dietary Fats; D050556:Fatty Acid-Binding Proteins; D012333:RNA, Messenger; D016718:Arachidonic Acid
keywords: Characterization; Cloning; Dietary arachidonic acid; Fatty acid-binding protein; Gene expression; Japanese seabass