title: Sevoflurane attenuates platelets activation of patients undergoing lung cancer surgery and suppresses platelets-induced invasion of lung cancer cells.
abstract: OBJECTIVE
  Platelets play a pivotal role in metastasis of tumor cells. The aim of this study is to explore the effects of sevoflurane and isoflurane on platelets activation of patients undergoing lung cancer surgery, and the effects of sevoflurane and isoflurane on platelets-induced invasion of lung cancer cells.
  
  
  METHODS
  Prospective and randomized study, and in vitro experiment.
  
  
  METHODS
  University-affiliated teaching hospital and laboratory.
  
  
  METHODS
  Forty-six patients scheduled for lung cancer radical surgery.
  
  
  METHODS
  Patients were randomized to two groups of 23 patients each and were received sevoflurane (group SEV) or isoflurane (group ISO) during surgery, respectively. In vitro, lung cancer cells were treated with platelets in the presence or absence anesthetics.
  
  
  METHODS
  Platelets activation were determined by detecting glycoproteinIIb/IIIa (GPIIb/IIIa), CD62P, and platelets aggregation rate (PAR) pre-, intra-, and postoperatively. Invasion ability of lung cancer cells were evaluated by Transwell assay.
  
  
  RESULTS
  The levels of GPIIb/IIIa, CD62P, and PAR were reduced markedly in group SEV during perioperative period compared with group ISO. In vitro, activated platelets contributed profoundly to the invasive ability of lung cancer cells. Sevoflurane, but not isoflurane, inhibited platelets-induced invasion of lung cancer cells. Furthermore, sevoflurane suppressed the platelets activity in vitro.
  
  
  CONCLUSIONS
  Sevoflurane attenuates platelets activation of patients undergoing lung cancer surgery. In vitro, sevoflurane suppresses platelets-induced invasion of lung cancer cells via decreasing platelets activity.
authors.affiliation: Department of Anesthesiology, Affiliated FoShan Hospital of SUN YAT-SEN University, 528000 Foshan, China.
authors.forename: Hua
authors.initials: H
authors.lastname: Liang
authors.affiliation: Department of Anesthesiology, Affiliated FoShan Hospital of SUN YAT-SEN University, 528000 Foshan, China.
authors.forename: Cheng Xiang
authors.initials: CX
authors.lastname: Yang
authors.affiliation: Department of Anesthesiology, Affiliated FoShan Hospital of SUN YAT-SEN University, 528000 Foshan, China. Electronic address: foshanzhangbin@126.com.
authors.forename: Bin
authors.initials: B
authors.lastname: Zhang
authors.affiliation: Department of Anesthesiology, Nanfang Hospital, Southern Medical University, 510515 Guangzhou, China.
authors.forename: Zhen Long
authors.initials: ZL
authors.lastname: Zhao
authors.affiliation: Department of Anesthesiology, Affiliated FoShan Hospital of SUN YAT-SEN University, 528000 Foshan, China.
authors.forename: Ji Ying
authors.initials: JY
authors.lastname: Zhong
authors.affiliation: Department of Anesthesiology, Affiliated FoShan Hospital of SUN YAT-SEN University, 528000 Foshan, China.
authors.forename: Xian Jie
authors.initials: XJ
authors.lastname: Wen
mesh_terms: D000072283:A549 Cells; D000368:Aged; D000768:Anesthesia, General; D018685:Anesthetics, Inhalation / Q000008:administration & dosage / Q000494:pharmacology*; D001792:Blood Platelets / Q000473:pathology*; D006801:Humans; D007530:Isoflurane / Q000008:administration & dosage / Q000494:pharmacology*; D008175:Lung Neoplasms / Q000097:blood / Q000276:immunology / Q000473:pathology* / Q000601:surgery; D008297:Male; D008738:Methyl Ethers / Q000008:administration & dosage / Q000494:pharmacology*; D008875:Middle Aged; D009361:Neoplasm Invasiveness / Q000276:immunology; D019007:P-Selectin / Q000032:analysis; D015539:Platelet Activation / Q000187:drug effects*; D010974:Platelet Aggregation / Q000187:drug effects; D019039:Platelet Glycoprotein GPIIb-IIIa Complex / Q000032:analysis; D011013:Pneumonectomy / Q000379:methods; D011446:Prospective Studies; D000077149:Sevoflurane; D013906:Thoracoscopy
chemical_list: D018685:Anesthetics, Inhalation; D008738:Methyl Ethers; D019007:P-Selectin; D019039:Platelet Glycoprotein GPIIb-IIIa Complex; C546937:SELP protein, human; D000077149:Sevoflurane; D007530:Isoflurane
keywords: Inhaled anesthetic; Lung cancer; Metastasis; Platelets