title: Responses of adventitial CD34+vascular wall-resident stem/progenitor cells and medial smooth muscle cells to carotid injury in rats.
abstract: Cell culture and carotid injury studies with SD rats were performed to investigate the roles of CD34+vascular wall-resident stem/progenitor cells (VRS/Pcs) and vascular smooth muscle cells (SMCs) in neointimal formation. In vitro, the media-isolated SM MHC+SMCs occupied 93.92±8.62% of total BrdU+cells, whereas the CD34+cells, only 2.61±0.82%, indicating that the cell expansion in SMC culture was attributed to SM MHC+SMCs. The adventitia-isolated CD34+VRS/Pcs responded to PDGF-BB by differentiating into SMC-like cells which expressed SM22α (an early stage SMC marker), but seldom SM MHC (a late stage SMC marker). In carotid injury model, the CD34+VRS/Pcs differentiated SMC-like cells migrated in very few numbers into only the outer layer of the media, and this was further confirmed by a cell tracking analysis. While the neointimal cells were consistently SM MHC+and CD34-SMCs during whole course of the post-injury remodeling. Thus it is speculated that the adventitial CD34+VRS/Pcs, at least in rat model, do not directly participate in neointimal formation, but function to maintain homeostasis of the media during injury-induced vascular wall remodeling.
authors.affiliation: Wuhan University School of Basic Medical Sciences, Wuhan, PR China. Electronic address: shenyan_ni@163.com.
authors.forename: Yan
authors.initials: Y
authors.lastname: Shen
authors.affiliation: Wuhan University School of Basic Medical Sciences, Wuhan, PR China. Electronic address: wuyan9811092@163.com.
authors.forename: Yan
authors.initials: Y
authors.lastname: Wu
authors.affiliation: Wuhan University School of Basic Medical Sciences, Wuhan, PR China. Electronic address: plsdd@163.com.
authors.forename: Yong
authors.initials: Y
authors.lastname: Zheng
authors.affiliation: Wuhan University School of Basic Medical Sciences, Wuhan, PR China. Electronic address: 158397540@qq.com.
authors.forename: Feng
authors.initials: F
authors.lastname: Ao
authors.affiliation: Wuhan University School of Basic Medical Sciences, Wuhan, PR China. Electronic address: 1359667062@qq.com.
authors.forename: Kai
authors.initials: K
authors.lastname: Kang
authors.affiliation: Wuhan University School of Basic Medical Sciences, Wuhan, PR China. Electronic address: wanyu@whu.edu.cn.
authors.forename: Yu
authors.initials: Y
authors.lastname: Wan
authors.affiliation: Wuhan University School of Basic Medical Sciences, Wuhan, PR China. Electronic address: s664184@163.com.
authors.forename: Jian
authors.initials: J
authors.lastname: Song
mesh_terms: D063194:Adventitia / Q000166:cytology*; D000818:Animals; D018952:Antigens, CD34 / Q000235:genetics / Q000378:metabolism; D020212:Carotid Artery Injuries / Q000473:pathology*; D002454:Cell Differentiation; D049109:Cell Proliferation; D002478:Cells, Cultured; D066026:Endothelial Progenitor Cells / Q000166:cytology* / Q000378:metabolism / Q000502:physiology; D004730:Endothelium, Vascular / Q000166:cytology; D009131:Muscle, Smooth, Vascular / Q000166:cytology*; D032389:Myocytes, Smooth Muscle / Q000166:cytology* / Q000378:metabolism / Q000502:physiology; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D018952:Antigens, CD34
keywords: Neointimal formation; Proliferation; Vascular smooth muscle cells; Vascular wall-resident stem/progenitor cells