title: LIN-23, an E3 Ubiquitin Ligase Component, Is Required for the Repression of CDC-25.2 Activity during Intestinal Development inCaenorhabditis elegans.
abstract: Caenorhabditis elegans(C. elegans) utilizes two different cell-cycle modes, binucleations during the L1 larval stage and endoreduplications at four larval moltings, for its postembryonic intestinal development. Previous genetic studies indicated that CDC-25.2 is specifically required for binucleations at the L1 larval stage and is repressed before endoreduplications. Furthermore, LIN-23, theC. elegansβ-TrCP ortholog, appears to function as a repressor of CDC-25.2 to prevent excess intestinal divisions. We previously reported that intestinal hyperplasia inlin-23(e1883)mutants was effectively suppressed by the RNAi depletion ofcdc-25.2. Nevertheless, LIN-23 targeting CDC-25.2 for ubiquitination as a component of E3 ubiquitin ligase has not yet been tested. In this study, LIN-23 is shown to be the major E3 ubiquitin ligase component, recognizing CDC-25.2 to repress their activities for proper transition of cell-cycle modes during theC. eleganspostembryonic intestinal development. In addition, for the first time that LIN-23 physically interacts with both CDC-25.1 and CDC-25.2 and facilitates ubiquitination for timely regulation of their activities during the intestinal development.
authors.affiliation: Department of Bioscience and Biotechnology, Konkuk University, Seoul 05029, Korea.
authors.forename: Miseol
authors.initials: M
authors.lastname: Son
authors.affiliation: Department of Bioscience and Biotechnology, Konkuk University, Seoul 05029, Korea.
authors.forename: Ichiro
authors.initials: I
authors.lastname: Kawasaki
authors.affiliation: Institute of Medical Science, Hanyang University College of Medicine, Seoul 04763, Korea.
authors.forename: Bong-Kyeong
authors.initials: BK
authors.lastname: Oh
authors.affiliation: Department of Bioscience and Biotechnology, Konkuk University, Seoul 05029, Korea.
authors.forename: Yhong-Hee
authors.initials: YH
authors.lastname: Shim
mesh_terms: D000818:Animals; D017173:Caenorhabditis elegans / Q000201:enzymology / Q000254:growth & development* / Q000378:metabolism*; D029742:Caenorhabditis elegans Proteins / Q000235:genetics / Q000378:metabolism*; D018797:Cell Cycle Proteins / Q000235:genetics / Q000378:metabolism*; D044783:F-Box Proteins / Q000235:genetics / Q000378:metabolism*; D006367:HeLa Cells; D006801:Humans; D007413:Intestinal Mucosa / Q000378:metabolism; D007422:Intestines / Q000201:enzymology / Q000254:growth & development; D010749:Phosphoprotein Phosphatases / Q000235:genetics / Q000378:metabolism*; D014162:Transfection; D044767:Ubiquitin-Protein Ligases / Q000235:genetics / Q000378:metabolism*
chemical_list: D029742:Caenorhabditis elegans Proteins; D018797:Cell Cycle Proteins; D044783:F-Box Proteins; C418507:lin-23 protein, C elegans; D044767:Ubiquitin-Protein Ligases; D010749:Phosphoprotein Phosphatases; C550003:cdc-25.2 protein, C elegans
keywords: C. elegans; CDC-25.2; E3 ubiquitin ligase; LIN-23; intestinal development