title: Integrated monitoring of chemicals and their effects on four sentinel species, Limanda limanda, Platichthys flesus, Nucella lapillus and Mytilus sp., in Seine Bay: A key step towards applying biological effects to monitoring.
abstract: The International workshop on Integrated Assessment of CONtaminants impacts on the North sea (ICON) provided a framework to validate the application of chemical and biological assessment thresholds (BACs and EACs) in the Seine Bay in France. Bioassays (oyster larval anomalies, Corophium arenarium toxicity assay and DR Calux) for sediment and biomarkers: ethoxyresorufin-O-deethylase (EROD) activity, acetylcholinesterase (AChE) activity, lysosomal membrane stability (LMS), DNA strand breaks using the Comet assay, DNA adducts, micronucleus (MN), PAH metabolites, imposex, intersex and fish external pathologies were analysed in four marine sentinel species (Platichthys flesus, Limanda limanda, Mytilus sp. and Nucella lapilus). Polycyclic aromatic hydrocarbons (PAHs), polychlorinated biphenyls (PCBs) and heavy metals were analysed in biota and sediment. Results for sediment and four species in 2008-2009 made it possible to quantify the impact of contaminants using thresholds (Environmental Assessment Criteria/EAC2008: 70% and EAC2009: 60%) and effects (EAC2008: 50% and EAC2009: 40%) in the Seine estuary. The Seine estuary is ranked among Europe's most highly polluted sites.
authors.affiliation: Department of Biogeochemistry and Ecotoxicology, Ifremer, Rue de l'Ile d'Yeu, BP 21105, 44311, Nantes cedex 03, France. Electronic address: tburgeot@ifremer.fr.
authors.forename: Thierry
authors.initials: T
authors.lastname: Burgeot
authors.affiliation: Department of Biogeochemistry and Ecotoxicology, Ifremer, Rue de l'Ile d'Yeu, BP 21105, 44311, Nantes cedex 03, France.
authors.forename: Farida
authors.initials: F
authors.lastname: Akcha
authors.affiliation: Department of Biogeochemistry and Ecotoxicology, Ifremer, Rue de l'Ile d'Yeu, BP 21105, 44311, Nantes cedex 03, France.
authors.forename: Dominique
authors.initials: D
authors.lastname: Ménard
authors.affiliation: Marine Scotland Science, Marine Laboratory, 375 Victoria Road, Aberdeen, AB11 9DB, UK.
authors.forename: Craig
authors.initials: C
authors.lastname: Robinson
authors.affiliation: Department of Biogeochemistry and Ecotoxicology, Ifremer, Rue de l'Ile d'Yeu, BP 21105, 44311, Nantes cedex 03, France.
authors.forename: Véronique
authors.initials: V
authors.lastname: Loizeau
authors.affiliation: Department of Biogeochemistry and Ecotoxicology, Ifremer, Rue de l'Ile d'Yeu, BP 21105, 44311, Nantes cedex 03, France.
authors.forename: Christophe
authors.initials: C
authors.lastname: Brach-Papa
authors.affiliation: Instituto Espagňol de Oceanografia, Oceanografic Centre of Murcia, Varadero 1, 30740, San Pedro del Pinatar, Murcia, Spain.
authors.forename: Concepción
authors.initials: C
authors.lastname: Martínez-Gòmez
authors.affiliation: Adn'Tox, GRECAN, Centre François Baclesse, Avenue du Général Harris, 14076, Caen Cedex 05, France.
authors.forename: Jérémie
authors.initials: J
authors.lastname: Le Goff
authors.affiliation: University of Bordeaux, Laboratory EPOC, UMR CNRS 5805, 33615, Pessac cedex, France.
authors.forename: Hélène
authors.initials: H
authors.lastname: Budzinski
authors.affiliation: University of Bordeaux, Laboratory EPOC, UMR CNRS 5805, 33615, Pessac cedex, France.
authors.forename: Karine
authors.initials: K
authors.lastname: Le Menach
authors.affiliation: University of Bordeaux, Laboratory EPOC, UMR CNRS 5805, 33615, Pessac cedex, France.
authors.forename: Jérome
authors.initials: J
authors.lastname: Cachot
authors.affiliation: Laboratory of Ecotoxicology (LEMA), UMR SEBIO, University of Le Havre, 25 Rue P. Lebon, 76058, Le Havre, France.
authors.forename: Christophe
authors.initials: C
authors.lastname: Minier
authors.affiliation: Federal Maritime and Hydrographic Agency of Germany (BSH), Benhard-Nocht-Straße 78, 20359, Hamburg, Germany.
authors.forename: Katja
authors.initials: K
authors.lastname: Broeg
authors.affiliation: Department of Biosciences, University of Oslo, Pb 1066, Blindern, N-0316, Oslo, Norway.
authors.forename: Ketil
authors.initials: K
authors.lastname: Hylland
mesh_terms: D000818:Animals; D061006:Bays; D019363:Cytochrome P-450 CYP1A1 / Q000378:metabolism; D004784:Environmental Monitoring / Q000379:methods*; D063366:Estuaries; D005432:Flounder / Q000378:metabolism; D005602:France; D049851:Gastropoda / Q000378:metabolism; D019216:Metals, Heavy / Q000378:metabolism; D049878:Mytilus / Q000378:metabolism; D011078:Polychlorinated Biphenyls / Q000378:metabolism; D011084:Polycyclic Aromatic Hydrocarbons / Q000378:metabolism; D000072739:Sentinel Species / Q000378:metabolism*; D014874:Water Pollutants, Chemical / Q000378:metabolism*
chemical_list: D019216:Metals, Heavy; D011084:Polycyclic Aromatic Hydrocarbons; D014874:Water Pollutants, Chemical; D011078:Polychlorinated Biphenyls; D019363:Cytochrome P-450 CYP1A1
keywords: Bioassays; Biomarkers; Chemical contamination; Integrated monitoring in chemistry and biology; Large geographical scale; Local study