title: Characterization of hemicelluloses from Neolamarckia cadamba (Rubiaceae) during xylogenesis.
abstract: In this study, hemicelluloses were isolated from the apical, middle and basal segments of N. cadamba using a 2N KOH extraction procedure. Chemical composition and structural characterization of the three hemicellulosic fractions obtained were comparatively investigated by a combination of HPLC, GPC, FTIR,1H,13C, HSQC NMR and TGA techniques. According to the sugar analysis and spectral results, (4-O-methyl) glucuronoxylan was the primary hemicellulose identified in the samples with trace levels of mannan also present. All of the three samples showed spherical polymers in 0.005M sodium phosphate solution (containing 0.02N NaCl). Xylan in the middle and basal segment stems had higher molecular weights. Our findings show that during xylogenesis in N. cadamba, changes are observed in xylan content and molecular weight.
authors.affiliation: State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, South China Agricultural University, Guangzhou, China; Provincial Key Laboratory of Agrobiology, Jiangsu Academy of Agricultural Sciences, China; Guangdong Key Laboratory for Innovative Development and Utilization of Forest Plant Germplasm, college of Forestry and Landscape Architecture, South China Agricultural University, Guangzhou, China; Guangdong Province Research Center of Woody Forage Engineering Technology, Guangzhou, China.
authors.forename: Xianhai
authors.initials: X
authors.lastname: Zhao
authors.affiliation: State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, South China Agricultural University, Guangzhou, China; Provincial Key Laboratory of Agrobiology, Jiangsu Academy of Agricultural Sciences, China; Guangdong Key Laboratory for Innovative Development and Utilization of Forest Plant Germplasm, college of Forestry and Landscape Architecture, South China Agricultural University, Guangzhou, China; Guangdong Province Research Center of Woody Forage Engineering Technology, Guangzhou, China.
authors.forename: Tingting
authors.initials: T
authors.lastname: Tong
authors.affiliation: Guangdong Key Laboratory for Innovative Development and Utilization of Forest Plant Germplasm, college of Forestry and Landscape Architecture, South China Agricultural University, Guangzhou, China; Guangdong Province Research Center of Woody Forage Engineering Technology, Guangzhou, China.
authors.forename: HuiLing
authors.initials: H
authors.lastname: Li
authors.affiliation: Guangdong Key Laboratory for Innovative Development and Utilization of Forest Plant Germplasm, college of Forestry and Landscape Architecture, South China Agricultural University, Guangzhou, China; Guangdong Province Research Center of Woody Forage Engineering Technology, Guangzhou, China.
authors.forename: Haojun
authors.initials: H
authors.lastname: Lu
authors.affiliation: State Key Laboratory of Pulp and Paper Engineering, South China University of Technology, Guangzhou, 510640, China.
authors.forename: Junli
authors.initials: J
authors.lastname: Ren
authors.affiliation: College of Materials and Energy, South China Agricultural University, Guangzhou, China.
authors.forename: Aiping
authors.initials: A
authors.lastname: Zhang
authors.affiliation: Guangdong Key Laboratory for Innovative Development and Utilization of Forest Plant Germplasm, college of Forestry and Landscape Architecture, South China Agricultural University, Guangzhou, China; Guangdong Province Research Center of Woody Forage Engineering Technology, Guangzhou, China.
authors.forename: Xiaomei
authors.initials: X
authors.lastname: Deng
authors.affiliation: State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, South China Agricultural University, Guangzhou, China; Guangdong Key Laboratory for Innovative Development and Utilization of Forest Plant Germplasm, college of Forestry and Landscape Architecture, South China Agricultural University, Guangzhou, China; Guangdong Province Research Center of Woody Forage Engineering Technology, Guangzhou, China.
authors.forename: Xiaoyang
authors.initials: X
authors.lastname: Chen
authors.affiliation: State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, South China Agricultural University, Guangzhou, China; Provincial Key Laboratory of Agrobiology, Jiangsu Academy of Agricultural Sciences, China; Guangdong Key Laboratory for Innovative Development and Utilization of Forest Plant Germplasm, college of Forestry and Landscape Architecture, South China Agricultural University, Guangzhou, China; Guangdong Province Research Center of Woody Forage Engineering Technology, Guangzhou, China. Electronic address: wuaimin@scau.edu.cn.
authors.forename: Ai-Min
authors.initials: AM
authors.lastname: Wu
mesh_terms: D009682:Magnetic Resonance Spectroscopy; D008970:Molecular Weight; D011134:Polysaccharides / Q000737:chemistry*; D019666:Rubiaceae / Q000737:chemistry*; D014990:Xylans / Q000096:biosynthesis / Q000737:chemistry* / Q000302:isolation & purification
chemical_list: D011134:Polysaccharides; D014990:Xylans; C007916:hemicellulose
keywords: (4-O-methyl) glucuronoxylan; Hemicellulose; N. cadamba; Structural characterization