title: Characterizing Auxin Response Circuits in Saccharomyces cerevisiae by Flow Cytometry.
abstract: Recapitulation of the nuclear auxin response pathway in Saccharomyces cerevisiae (yeast) provides a means to functionally assay the contribution of individual signaling components to response dynamics. Here, we describe a time course assay for characterizing auxin response circuits using flow cytometry. This method allows for quantitative measurements of the dynamic response of up to 12 circuits (strains) at once. We also describe a steady-state assay and how to utilize an R package we developed to facilitate data analysis.
authors.affiliation: Department of Biology, University of Washington, 24 Kincaid Hall, Seattle, WA, 98195, USA.
authors.forename: Edith
authors.initials: E
authors.lastname: Pierre-Jerome
authors.affiliation: Department of Biology, University of Washington, 24 Kincaid Hall, Seattle, WA, 98195, USA.
authors.forename: R Clay
authors.initials: RC
authors.lastname: Wright
authors.affiliation: Department of Biology, University of Washington, 24 Kincaid Hall, Seattle, WA, 98195, USA. jn7@uw.edu.
authors.forename: Jennifer L
authors.initials: JL
authors.lastname: Nemhauser
mesh_terms: D005434:Flow Cytometry; D007210:Indoleacetic Acids / Q000378:metabolism*; D010937:Plant Growth Regulators / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D015398:Signal Transduction / Q000502:physiology
chemical_list: D007210:Indoleacetic Acids; D010937:Plant Growth Regulators
keywords: Budding yeast; Fluorescent reporters; Signaling dynamics; Synthetic biology