title: Recent advances in high-throughput13C-fluxomics.
abstract: The rise of high throughput (HT) strain engineering tools accompanying the area of synthetic biology is supporting the generation of a large number of microbial cell factories. A current bottleneck in process development is our limited capacity to rapidly analyze the metabolic state of the engineered strains, and in particular their intracellular fluxes. HT13C-fluxomics workflows have not yet become commonplace, despite the existence of several HT tools at each of the required stages. This includes cultivation and sampling systems, analytics for isotopic analysis, and software for data processing and flux calculation. Here, we review recent advances in the field and highlight bottlenecks that must be overcome to allow the emergence of true HT13C-fluxomics workflows.
authors.affiliation: LISBP, Université de Toulouse, CNRS, INRA, INSA, Toulouse, France.
authors.forename: Stéphanie
authors.initials: S
authors.lastname: Heux
authors.affiliation: LISBP, Université de Toulouse, CNRS, INRA, INSA, Toulouse, France.
authors.forename: Cécilia
authors.initials: C
authors.lastname: Bergès
authors.affiliation: LISBP, Université de Toulouse, CNRS, INRA, INSA, Toulouse, France.
authors.forename: Pierre
authors.initials: P
authors.lastname: Millard
authors.affiliation: LISBP, Université de Toulouse, CNRS, INRA, INSA, Toulouse, France.
authors.forename: Jean-Charles
authors.initials: JC
authors.lastname: Portais
authors.affiliation: LISBP, Université de Toulouse, CNRS, INRA, INSA, Toulouse, France. Electronic address: fabien.letisse@insa-toulouse.fr.
authors.forename: Fabien
authors.initials: F
authors.lastname: Létisse
mesh_terms: D001419:Bacteria / Q000378:metabolism*; D064688:Metabolic Flux Analysis / Q000379:methods*; D055442:Metabolome; D012984:Software; D058615:Synthetic Biology / Q000379:methods*