title: Association of TET1 expression with colorectal cancer progression.
abstract: OBJECTIVE
  The ten-eleven translocation (TET) proteins, as methylcytosine dioxygenases, catalyze 5-methylcytosine (5mC) into 5-hydroxymethylcytosine (5hmC). The altered expression of TET1 disrupts the balance between DNA methylation and demethylation. This alteration has been reported to be associated with carcinogenesis in various malignancies. The aim of the present study was to investigate changes in expression and the role of TET1 in colorectal cancer (CRC).
  
  
  METHODS
  A total of 109 CRC patients who underwent radical surgical colon resection were enrolled. The QuantiGene Plex Assay was used to detect the expression of TET1 in CRC tissues and matching adjacent normal tissues. We analyzed the associations between TET1 expression levels and various clinicopathologic features of CRC. TET1 overexpression and depletion cells were constructed to investigate its biological role in CRC.
  
  
  RESULTS
  Compared to normal tissues, the expression level of TET1 in CRC was significantly lower. The ratio of TET1 in CRC tissues to that in adjacent normal tissues (C/N-TET1) was an independent overall survival predictive factor. Moreover, in vitro studies showed that TET1 could inhibit cell growth and promote cell metastasis and invasion.
  
  
  CONCLUSIONS
  These findings indicated that TET1 played a multifaceted role in the pathogenesis of CRC, and thereby resulting in multiple effects on tumor progression.
authors.affiliation: a Department of Pathology , Zhejiang University School of Medicine , Hangzhou , China.|b Key Laboratory of Disease Proteomics of Zhejiang Province , Hangzhou , China.|c Pathology Department , Zhejiang Cancer Hospital , Hangzhou , China.
authors.forename: Yiping
authors.initials: Y
authors.lastname: Tian
authors.affiliation: d Department of Epidemiology and Biostatistics , Zhejiang University School of Public Health , Hangzhou , China.
authors.forename: Feixia
authors.initials: F
authors.lastname: Pan
authors.affiliation: d Department of Epidemiology and Biostatistics , Zhejiang University School of Public Health , Hangzhou , China.
authors.forename: Xiaohui
authors.initials: X
authors.lastname: Sun
authors.affiliation: e Department of Pathology , Taizhou Hospital , Linhai , China.
authors.forename: Meifu
authors.initials: M
authors.lastname: Gan
authors.affiliation: f Medical Research Center, Taizhou Hospital of Zhejiang Province, Wenzhou Medical University , Linhai , China.
authors.forename: Aifen
authors.initials: A
authors.lastname: Lin
authors.affiliation: a Department of Pathology , Zhejiang University School of Medicine , Hangzhou , China.|b Key Laboratory of Disease Proteomics of Zhejiang Province , Hangzhou , China.
authors.forename: Dandan
authors.initials: D
authors.lastname: Zhang
authors.affiliation: c Pathology Department , Zhejiang Cancer Hospital , Hangzhou , China.
authors.forename: Yimin
authors.initials: Y
authors.lastname: Zhu
authors.affiliation: a Department of Pathology , Zhejiang University School of Medicine , Hangzhou , China.|b Key Laboratory of Disease Proteomics of Zhejiang Province , Hangzhou , China.
authors.forename: Maode
authors.initials: M
authors.lastname: Lai
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002465:Cell Movement; D049109:Cell Proliferation; D002681:China; D015179:Colorectal Neoplasms / Q000235:genetics* / Q000473:pathology*; D019175:DNA Methylation; D018450:Disease Progression; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D053208:Kaplan-Meier Estimate; D008297:Male; D008875:Middle Aged; D006899:Mixed Function Oxygenases / Q000235:genetics / Q000378:metabolism*; D016016:Proportional Hazards Models; D011518:Proto-Oncogene Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D011518:Proto-Oncogene Proteins; D006899:Mixed Function Oxygenases; C462539:TET1 protein, human
keywords: TET protein; colorectal cancer; metastases; prognosis; proliferation