title: Bioremediation of hydrocarbon degradation in a petroleum-contaminated soil and microbial population and activity determination.
abstract: Bioremediation of hydrocarbon degradation in petroleum-polluted soil is carried out by various microorganisms. However, little information is available for the relationships between hydrocarbon degradation rates in petroleum-contaminated soil and microbial population and activity in laboratory assay. In a microcosm study, degradation rate and efficiency of total petroleum hydrocarbons (TPH), alkanes, and polycyclic aromatic hydrocarbons (PAH) in a petroleum-contaminated soil were determined using an infrared photometer oil content analyzer and a gas chromatography mass spectrometry (GC-MS). Also, the populations of TPH, alkane, and PAH degraders were enumerated by a modified most probable number (MPN) procedure, and the hydrocarbon degrading activities of these degraders were determined by the Biolog (MT2) MicroPlates assay. Results showed linear correlations between the TPH and alkane degradation rates and the population and activity increases of TPH and alkane degraders, but no correlation was observed between the PAH degradation rates and the PAH population and activity increases. Petroleum hydrocarbon degrading microbial population measured by MPN was significantly correlated with metabolic activity in the Biolog assay. The results suggest that the MPN procedure and the Biolog assay are efficient methods for assessing the rates of TPH and alkane, but not PAH, bioremediation in oil-contaminated soil in laboratory.
authors.affiliation: School of Environmental and Municipal Engineering, Xi'an University of Architecture and Technology, No.13 Yanta Road, Xi'an, Shaanxi Province 710055, China.
authors.forename: Manli
authors.initials: M
authors.lastname: Wu
authors.affiliation: School of Environmental and Municipal Engineering, Xi'an University of Architecture and Technology, No.13 Yanta Road, Xi'an, Shaanxi Province 710055, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Li
authors.affiliation: School of Environment and Natural Resources, The Ohio State University, Ohio Agricultural Research and Development Center, 1680 Madison Avenue, Wooster, OH 44691, USA.
authors.forename: Warren A
authors.initials: WA
authors.lastname: Dick
authors.affiliation: School of Environmental and Municipal Engineering, Xi'an University of Architecture and Technology, No.13 Yanta Road, Xi'an, Shaanxi Province 710055, China.
authors.forename: Xiqiong
authors.initials: X
authors.lastname: Ye
authors.affiliation: School of Environmental and Municipal Engineering, Xi'an University of Architecture and Technology, No.13 Yanta Road, Xi'an, Shaanxi Province 710055, China.
authors.forename: Kaili
authors.initials: K
authors.lastname: Chen
authors.affiliation: School of Environment and Natural Resources, The Ohio State University, Ohio Agricultural Research and Development Center, 1680 Madison Avenue, Wooster, OH 44691, USA.
authors.forename: David
authors.initials: D
authors.lastname: Kost
authors.affiliation: School of Environment and Natural Resources, The Ohio State University, Ohio Agricultural Research and Development Center, 1680 Madison Avenue, Wooster, OH 44691, USA. Electronic address: chen.280@osu.edu.
authors.forename: Liming
authors.initials: L
authors.lastname: Chen
mesh_terms: D000473:Alkanes; D001673:Biodegradation, Environmental; D006838:Hydrocarbons / Q000378:metabolism; D010578:Petroleum / Q000032:analysis*; D059392:Petroleum Pollution / Q000032:analysis*; D011084:Polycyclic Aromatic Hydrocarbons; D012987:Soil; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000032:analysis*
chemical_list: D000473:Alkanes; D006838:Hydrocarbons; D010578:Petroleum; D011084:Polycyclic Aromatic Hydrocarbons; D012987:Soil; D012989:Soil Pollutants
keywords: Biolog assay; Bioremediation; Hydrocarbon degradation efficiency; Microbial population; Petroleum-contaminated soil