title: Real-Time Analysis of the Apical Hook Development.
abstract: Mechanisms for cell protection are essential for survival of multicellular organisms. In plants, the apical hook, which is transiently formed in darkness when the germinating seedling penetrates towards the soil surface, plays such protective role and shields the vitally important shoot apical meristem and cotyledons from damage. The apical hook is formed by bending of the upper hypocotyl soon after germination, and it is maintained in a closed stage while the hypocotyl continues to penetrate through the soil and rapidly opens when exposed to light in proximity of the soil surface. To uncover the complex molecular network orchestrating this spatiotemporally tightly coordinated process, monitoring of the apical hook development in real time is indispensable. Here we describe an imaging platform that enables high-resolution kinetic analysis of this dynamic developmental process.
authors.affiliation: Department of Life Sciences, Institute of Science and Technology Austria, Am Campus 1, 3400, Klosterneuburg, Austria.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Zhu
authors.affiliation: Institut für Genetik, Heinrich-Heine-Universität Düsseldorf, Düsseldorf, Germany.
authors.forename: Petra
authors.initials: P
authors.lastname: Žádníková
authors.affiliation: Laboratory of Functional Plant Biology, Department of Physiology, Ghent University, Ghent, Belgium.
authors.forename: Dajo
authors.initials: D
authors.lastname: Smet
authors.affiliation: Laboratory of Functional Plant Biology, Department of Physiology, Ghent University, Ghent, Belgium.
authors.forename: Dominique
authors.initials: D
authors.lastname: Van Der Straeten
authors.affiliation: Department of Life Sciences, Institute of Science and Technology Austria, Am Campus 1, 3400, Klosterneuburg, Austria. eva.benkova@ist.ac.at.
authors.forename: Eva
authors.initials: E
authors.lastname: Benková
mesh_terms: D017360:Arabidopsis / Q000235:genetics / Q000254:growth & development*; D029681:Arabidopsis Proteins / Q000235:genetics; D003624:Darkness; D018507:Gene Expression Regulation, Developmental / Q000235:genetics; D018506:Gene Expression Regulation, Plant / Q000235:genetics; D017343:Genes, Plant / Q000235:genetics; D018525:Germination / Q000235:genetics; D018546:Hypocotyl / Q000235:genetics / Q000254:growth & development*; D007700:Kinetics; D008027:Light; D018519:Meristem / Q000235:genetics / Q000254:growth & development*; D010937:Plant Growth Regulators / Q000235:genetics; D030821:Plants, Genetically Modified / Q000235:genetics / Q000254:growth & development; D036226:Seedlings / Q000235:genetics / Q000254:growth & development
chemical_list: D029681:Arabidopsis Proteins; D010937:Plant Growth Regulators
keywords: Apical hook development; Differential growth; Hormonal cross talk; Phenotype analysis; Real-time imaging