title: MicroRNA-215 enhances invasion and migration by targeting retinoblastoma tumor suppressor gene 1 in high-grade glioma.
abstract: OBJECTIVE
  To elucidate the molecular mechanism of microRNA-215 (miR-215) in the migration and invasion of high grade glioma.
  
  
  RESULTS
  42 Patients were analysed for clinicopathological characteristics. qRT-PCR showed that miR-215 was up-regulated in glioma tissues compared with non-neoplastic brain tissues (P < 0.05). The up-regulated miR-215 was closely associated with high grade glioma (P < 0.01) and poor overall survival (P < 0.01). Transwell assay showed that re-expression of miR-215 enhanced migration and invasion of glioma cells. miR-215 also down-regulated retinoblastoma tumor suppressor gene 1 (RB1) expression by targeting its 3'-UTR. Reversely, re-expression of RB1 inhibited partial effect of miR-215 on migration and invasion in vitro.
  
  
  CONCLUSIONS
  Re-expression of miR-215 promoted cell migration and invasion of glioma by targeting RB1. miR-215 can thus be used as a biomarker for tumor progression and prognosis in human high grade glioma.
authors.affiliation: Department of Neurosurgery, Qilu Hospital of Shandong University, 107 of Wenhua Xi Road Lixia District, Jinan, 250012, Shandong, People's Republic of China.|Department of Neurosurgery, Jining No.1 People's Hospital, Jining, Shandong, People's Republic of China.
authors.forename: Yuzhen
authors.initials: Y
authors.lastname: Wei
authors.affiliation: Department of Endocrinology, Jining No.1 People's Hospital, Jining, 272 011, Shandong, People's Republic of China.
authors.forename: Jianjing
authors.initials: J
authors.lastname: Sun
authors.affiliation: Department of Neurosurgery, Qilu Hospital of Shandong University, 107 of Wenhua Xi Road Lixia District, Jinan, 250012, Shandong, People's Republic of China. lixingangmed@yeah.net.|Brain Science Research Institute, Shandong University, Jinan, Shandong, People's Republic of China. lixingangmed@yeah.net.
authors.forename: Xingang
authors.initials: X
authors.lastname: Li
mesh_terms: D000328:Adult; D001932:Brain Neoplasms / Q000235:genetics* / Q000473:pathology*; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics / Q000502:physiology*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics / Q000502:physiology; D005910:Glioma / Q000235:genetics* / Q000473:pathology*; D006801:Humans; D066298:In Vitro Techniques; D008297:Male; D035683:MicroRNAs / Q000235:genetics* / Q000502:physiology; D008875:Middle Aged
chemical_list: C553667:MIRN215 microRNA, human; D035683:MicroRNAs
keywords: Glioma; Invasion; Migration; Retinoblastoma tumor suppressor gene 1; miRNA-215