title: In-vivo study for anti-hyperglycemic potential of aqueous extract of Basil seeds (Ocimum basilicum Linn) and its influence on biochemical parameters, serum electrolytes and haematological indices.
abstract: The study introduced anti-hyperglycemic influence of aqueous extract of Ocimum basilicum seeds (AEOBS) in Streptozotocin (STZ) induced diabetic rats and estimating its potential to ameliorate altered level of biochemical parameters, serum electrolytes level and haematological indices along with its effect on body weight of treated rats. The albino rats were selected to observe oral glucose tolerance test by oral intake of aq. glucose solution (4g/kg, body weight) in normal rats and estimation of blood glucose level after administration of AEOBS at 250mg/kg, 500mg/kg and standard drug glibenclamide at 0.6mg/kg, body weight. Antidiabetic activity was evaluated in chronic study models by STZ induced diabetes in rats followed by blood glucose estimation. Chronic study model was selected to carry out further studies to evaluate the effect of AEOBS at 250mg/kg, 500mg/kg and standard drug on body weight, alterations in biochemical parameters including AST, ALT, ALP, total bilirubin and total protein, alterations in serum electrolytes like Na+, K+, Cl-, HCO3-along with estimation of haematological indices like red blood cells (RBC), white blood cells (WBC), hemoglobin (Hb), lymphocytes, neutrophils, eosinophils, monocytes and basophils. AEOBS significantly reduced the blood glucose level of diabetic rats at both doses. Body weight was also improved significantly. Similarly, the levels of biochemical parameters, serum electrolytes, and haematological indices were significantly ameliorated at both doses of AEOBS. The histopathological results revealed reconstitution of pancreatic islets towards normal cellular architecture in rats treated with AEOBS. The results illustrated that AEOBS have eminent antidiabetic potential in STZ effectuated diabetes in rats and can be extensively used for the treatment of diabetes mellitus-II and its associated complications including anaemia, diabetic nephropathy, liver dysfunction, and immunosuppression.
authors.affiliation: College of Pharmacy, University of Sharjah, Sharjah 27272, United Arab Emirates; Moradabad Educational Trust, Group of Institutions Faculty of Pharmacy, Moradabad 244001, Uttar Pradesh, India. Electronic address: sachin.nehra84@gmail.com.
authors.forename: Sachin
authors.initials: S
authors.lastname: Chaudhary
authors.affiliation: Shree Dev Bhoomi Institute of Science Education and Technology, Dehradun 248001, Uttarakhand, India.
authors.forename: Amit
authors.initials: A
authors.lastname: Semwal
authors.affiliation: Moradabad Educational Trust, Group of Institutions Faculty of Pharmacy, Moradabad 244001, Uttar Pradesh, India.
authors.forename: Hitesh
authors.initials: H
authors.lastname: Kumar
authors.affiliation: Moradabad Educational Trust, Group of Institutions Faculty of Pharmacy, Moradabad 244001, Uttar Pradesh, India.
authors.forename: Harish Chandra
authors.initials: HC
authors.lastname: Verma
authors.affiliation: College of Pharmacy, Teerthanker Mahaveer University, Moradabad 244001, Uttar Pradesh, India.
authors.forename: Amit
authors.initials: A
authors.lastname: Kumar
mesh_terms: D000818:Animals; D003921:Diabetes Mellitus, Experimental / Q000097:blood* / Q000188:drug therapy; D004573:Electrolytes / Q000037:antagonists & inhibitors / Q000097:blood*; D005951:Glucose Tolerance Test / Q000379:methods; D007004:Hypoglycemic Agents / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use*; D018649:Ocimum basilicum; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use*; D051381:Rats; D012639:Seeds
chemical_list: D004573:Electrolytes; D007004:Hypoglycemic Agents; D010936:Plant Extracts
keywords: Antihyperglycemic; Aqueous extract; Glibenclamide; Ocimum basilicum; Streptozotocin