title: [Expression of CD10 in tumor-associated fibroblast of cancerized or recurrent colorectal adenomas].
abstract: Objective:To investigate the expression of CD10 in tumor-associated fibroblasts (TAF) in colorectal adenomas and its relation to cancerization and recurrence of adenoma.Methods:Tissue samples of low-grade adenoma (n=50), high-grade adenoma (n=50) and colorectal adenocarcinoma (n=50) were collected, and tissue samples at the distal margin of corresponding colorectal lesions were taken as controls. The expression of CD10 in the stromal TAFs, and the expressions of β-catenin, Ki-67, p53 and CyclinD1 in tumor cells were detected by immunohistochemistry (Envision). The correlation of CD10 expression in stromal TAFs with the expressions of β-catenin, Ki-67, p53 and CyclinD1 in tumor cells was analyzed by Spearmen. One hundred samples of low-grade colorectal adenoma were collected, including 57 non-recurrent cases and 43 recurrent cases (16 cases of recurrent adenoma and 27 cases of recurrent adenocarcinoma); the expression of stromal TAF CD10 were determined and compared among groups.Results:There was no TAF in normal colorectal mucosa. The expression rates of TAF CD10 in low-grade adenoma, high-grade adenoma and colorectal adenocarcinoma were 22%, 50% and 78%, respectively (allP<0.05). The expression of Ki-67 and β-catenin in low-grade adenoma, high-grade adenoma, colorectal adenocarcinoma was on a rising trend (allP<0.01). The expression of CyclinD1 in high-grade adenoma was higher than that in colorectal adenocarcinoma and low-grade adenoma (allP>0.05). The expression of p53 in colorectal adenocarcinoma and high-grade adenoma was higher than that in low grade adenoma (allP<0.01). The expression of TAF CD10 was correlated with the expression of p53, Ki-67 and β-catenin-nucleus(r=0.264、0.307、0.320, allP<0.01),but not correlated with CyclinD1 and β-catenin-membrane (r=0.012、-0.073, allP>0.05). The TAF CD10 level was significantly higher in low-grade adenoma with recurrence than that in those without recurrence (P<0.05).The expression of CD10 in recurrent colorectal adenocarcinoma was higher than that in recurrent adenoma (P<0.05).Conclusion:The expression of TAF CD10 is increased gradually in the process of adenoma-cancer, indicating that it may play an important role in the canceration of adenoma. Adenomas with high expression of CD10 TAF are likely to be recurrent and cancerized, and detection of TAF CD10 combined with p53, Ki-67 and β-catenin may be of value in predicting canceration or recurrence of colorectal adenoma.
authors.affiliation: Department of Pathology and Pathophysiology, Zhejiang University School of Medicine, Hangzhou 310058, China;Department of Pathology, Yinzhou Second Hospital of Ningbo City, Ningbo 315100, China.
authors.forename: Jiangjiang
authors.initials: J
authors.lastname: Zheng
authors.affiliation: Department of Pathology and Pathophysiology, Zhejiang University School of Medicine, Hangzhou 310058, China;Department of Pathology, Yinzhou Second Hospital of Ningbo City, Ningbo 315100, China.
authors.forename: Yin
authors.initials: Y
authors.lastname: Zhu
authors.affiliation: Department of Pathology, Yinzhou Second Hospital of Ningbo City, Ningbo 315100, China.
authors.forename: Changshui
authors.initials: C
authors.lastname: Li
authors.affiliation: Department of Pathology, Yinzhou Second Hospital of Ningbo City, Ningbo 315100, China.
authors.forename: Yinya
authors.initials: Y
authors.lastname: Li
authors.affiliation: Department of Pathology and Pathophysiology, Zhejiang University School of Medicine, Hangzhou 310058, China.
authors.forename: Qianqian
authors.initials: Q
authors.lastname: Nie
authors.affiliation: Department of Pathology and Pathophysiology, Zhejiang University School of Medicine, Hangzhou 310058, China.
authors.forename: Ziling
authors.initials: Z
authors.lastname: Zhu
authors.affiliation: Department of Pathology and Pathophysiology, Zhejiang University School of Medicine, Hangzhou 310058, China. hongdeng@zju.edu.cn.
authors.forename: Hong
authors.initials: H
authors.lastname: Deng
mesh_terms: D000230:Adenocarcinoma / Q000737:chemistry* / Q000235:genetics*; D000236:Adenoma / Q000737:chemistry* / Q000235:genetics*; D014408:Biomarkers, Tumor / Q000032:analysis*; D000072645:Cancer-Associated Fibroblasts / Q000737:chemistry*; D063646:Carcinogenesis / Q000737:chemistry; D015179:Colorectal Neoplasms / Q000737:chemistry* / Q000235:genetics*; D019938:Cyclin D1 / Q000032:analysis; D018450:Disease Progression; D006801:Humans; D007150:Immunohistochemistry; D019394:Ki-67 Antigen / Q000032:analysis; D060787:Neoplasm Grading; D009364:Neoplasm Recurrence, Local / Q000737:chemistry; D015260:Neprilysin / Q000032:analysis*; D011237:Predictive Value of Tests; D016159:Tumor Suppressor Protein p53 / Q000032:analysis; D051176:beta Catenin / Q000032:analysis
chemical_list: D014408:Biomarkers, Tumor; D019394:Ki-67 Antigen; D016159:Tumor Suppressor Protein p53; D051176:beta Catenin; D019938:Cyclin D1; D015260:Neprilysin