title: Transmitting characters of individual E chromosomes of Thinopyrum elongatum in Triticum turgidum background.
abstract: The transmission patterns of Thinopyrum elongatum chromosomes in the background of Triticum turgidum were investigated through cytogenetic and molecular marker analysis based on the F2and F3plants derived from a cross between Triticum trititrigia (AABBEE) and T. turgidum L. ssp. durum (AABB). An additional objective was to develop durum-Th. elongatum E chromosome addition lines. Among 218 F2plants, individuals with 2n=28 accounted for 41.7%, those with 2n=29 accounted for 18.3%, and the remaining 40.0% had 2n=31-42. Molecular marker analysis of the F2monosomic addition plants with 2n=29 showed significant differences in transmission rate among Th. elongatum chromosomes. Chromosome 1E had the highest transmission rates, while 3E and 6E had the lowest. Among F2double monosomic addition plants with 2n=30, joint transmission was frequent between 1E, 4E, 7E and 5E; 6E was less frequent in joint transmission with other chromosomes, while 2E and 3E never transmitted along with other chromosomes. Among F3plants derived from F2monosomic addition plants, the transmission rates of E chromosomes varied widely, from the minimum of 9.1% for 6E to the maximum of 27.5% for 1E, consistent with observations from F2plants. A full set of durum-Th. elongatum 1E-7E monosomic addition lines and several disomic addition lines were selected from F3plants, and all monosomic addition plants were fertile. These chromosome E addition lines will be useful intermediate materials for developing substitution lines and translocation lines.
authors.affiliation: 1. College of Bioscience and Biotechnology, Yangzhou University, Yangzhou 225009, China; 2. Yangzhou Polytechnic College, Yangzhou 225012, China.
authors.forename: Hai-feng
authors.initials: HF
authors.lastname: Li
authors.affiliation: College of Bioscience and Biotechnology, Yangzhou University, Yangzhou 225009, China.
authors.forename: Hui-ping
authors.initials: HP
authors.lastname: Liu
authors.affiliation: College of Bioscience and Biotechnology, Yangzhou University, Yangzhou 225009, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Dai
authors.affiliation: Jiangsu Provincial Key Lab for Agrobiology, Nanjing 210014, China.
authors.forename: Shuai
authors.initials: S
authors.lastname: Huang
authors.affiliation: Yangzhou Polytechnic College, Yangzhou 225012, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Zhang
authors.affiliation: College of Bioscience and Biotechnology, Yangzhou University, Yangzhou 225009, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Gao
authors.affiliation: College of Bioscience and Biotechnology, Yangzhou University, Yangzhou 225009, China.
authors.forename: Jianmin
authors.initials: J
authors.lastname: Chen
mesh_terms: D032461:Chromosomes, Plant / Q000235:genetics*; D006824:Hybridization, Genetic; D006109:Poaceae / Q000235:genetics*; D014178:Translocation, Genetic; D014908:Triticum / Q000235:genetics*