title: Soluble CD200 Correlates With Interleukin-6 Levels in Sera of COPD Patients: Potential Implication of the CD200/CD200R Axis in the Disease Course.
abstract: COPD represents a multifactorial lung disorder with high morbidity and mortality. Despite intensive research concerning the underlying disease mechanisms, the involvement of the CD200/CD200R axis in supporting or preventing the onset of COPD has not yet been addressed. Since the CD200/CD200R axis is crucially implicated in the maintenance of pulmonary immune homeostasis, we hypothesized that it might be involved in controlling the onset of COPD.
  
  
  
  To address this, we analyzed the serum samples from COPD patients and normal controls for soluble (s) CD200 and correlated the data to COPD-relevant clinical parameters. In addition, basic studies were conducted in CD200-deficient and wild-type mice in which COPD-like inflammation was induced with elastase/LPS followed by lung and serum component analysis.
  
  
  
  We observed a positive correlation between serum sCD200 and IL-6 levels as well as a trend toward a negative correlation of sCD200 with vitamin D3 in COPD patients. Further investigations in mice revealed that despite elevated serum concentration of MMP-9 in CD200KO mice, the early onset of COPD-like lung inflammation was similar in CD200-deficient and wild-type animals in terms of immune cell infiltration, emphysematous changes, and mucus overproduction.
  
  
  
  While our murine studies suggest that the co-inhibitory molecule CD200 does not appear to play a prominent role in the early onset of COPD-like features, correlation of sCD200 serum levels with COPD-related parameters in humans with established disease revealed that the CD200/CD200R axis may be mechanistically linked to the disease course in COPD patients.
authors.affiliation: Immune Regulation Group, Helmholtz Centre for Infection Research, Inhoffenstrasse 7, 38124, Braunschweig, Germany. priya.sakthivel@helmholtz-hzi.de.|Infection Immunology Group, Institute of Medical Microbiology, Infection Control and Prevention, Otto-von-Guericke University Magdeburg, Magdeburg, Germany. priya.sakthivel@helmholtz-hzi.de.
authors.forename: Priya
authors.initials: P
authors.lastname: Sakthivel
authors.affiliation: Department of Veterinary Medicine, Institute of Veterinary Pathology, Freie Universität Berlin, Berlin, Germany.
authors.forename: Angele
authors.initials: A
authors.lastname: Breithaupt
authors.affiliation: Immune Regulation Group, Helmholtz Centre for Infection Research, Inhoffenstrasse 7, 38124, Braunschweig, Germany.|Infection Immunology Group, Institute of Medical Microbiology, Infection Control and Prevention, Otto-von-Guericke University Magdeburg, Magdeburg, Germany.
authors.forename: Marcus
authors.initials: M
authors.lastname: Gereke
authors.affiliation: School of Clinical Sciences, University of Bristol, Bristol, BS8 1TD, UK.|School of Cellular and Molecular Medicine, University of Bristol, Bristol, BS8 1TD, UK.|National Institute for Health Research Biomedical Research Centre at Moorfields Eye Hospital NHS Foundation Trust and UCL Institute of Ophthalmology, London, EC1V 2PD, UK.
authors.forename: David A
authors.initials: DA
authors.lastname: Copland
authors.affiliation: Department of Gastroenterology, Hepatology and Infectious Diseases, Otto-von-Guericke-University Magdeburg, University Hospital, Magdeburg, Germany.
authors.forename: Christian
authors.initials: C
authors.lastname: Schulz
authors.affiliation: Department of Veterinary Medicine, Institute of Veterinary Pathology, Freie Universität Berlin, Berlin, Germany.
authors.forename: Achim D
authors.initials: AD
authors.lastname: Gruber
authors.affiliation: School of Clinical Sciences, University of Bristol, Bristol, BS8 1TD, UK.|School of Cellular and Molecular Medicine, University of Bristol, Bristol, BS8 1TD, UK.|National Institute for Health Research Biomedical Research Centre at Moorfields Eye Hospital NHS Foundation Trust and UCL Institute of Ophthalmology, London, EC1V 2PD, UK.
authors.forename: Andrew D
authors.initials: AD
authors.lastname: Dick
authors.affiliation: Department of Pulmonology, Otto-von-Guericke-University Magdeburg, University Hospital, Magdeburg, Germany.
authors.forename: Jens
authors.initials: J
authors.lastname: Schreiber
authors.affiliation: Immune Regulation Group, Helmholtz Centre for Infection Research, Inhoffenstrasse 7, 38124, Braunschweig, Germany.|Infection Immunology Group, Institute of Medical Microbiology, Infection Control and Prevention, Otto-von-Guericke University Magdeburg, Magdeburg, Germany.
authors.forename: Dunja
authors.initials: D
authors.lastname: Bruder
mesh_terms: D000368:Aged; D000818:Animals; D015703:Antigens, CD / Q000097:blood* / Q000235:genetics*; D000954:Antigens, Surface / Q000378:metabolism; D016022:Case-Control Studies; D002762:Cholecalciferol / Q000097:blood; D004195:Disease Models, Animal; D005260:Female; D006801:Humans; D015850:Interleukin-6 / Q000097:blood; D008070:Lipopolysaccharides; D008214:Lymphocytes / Q000473:pathology; D016676:Macrophages, Alveolar / Q000473:pathology; D008297:Male; D020780:Matrix Metalloproteinase 9 / Q000097:blood; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D008875:Middle Aged; D009504:Neutrophils / Q000473:pathology; D064446:Orexin Receptors; D010196:Pancreatic Elastase; D029424:Pulmonary Disease, Chronic Obstructive / Q000097:blood* / Q000139:chemically induced / Q000473:pathology*; D011956:Receptors, Cell Surface / Q000378:metabolism
chemical_list: D015703:Antigens, CD; D000954:Antigens, Surface; C477337:CD200R1 protein, human; D015850:Interleukin-6; D008070:Lipopolysaccharides; D064446:Orexin Receptors; D011956:Receptors, Cell Surface; D002762:Cholecalciferol; D010196:Pancreatic Elastase; D020780:Matrix Metalloproteinase 9; C501830:Mmp9 protein, mouse; C042739:antigens, CD200
keywords: CD200-deficient mice; CD200/CD200R axis; COPD; LPS/elastase model; MMP-9