title: The Neurospora Transcription Factor ADV-1 Transduces Light Signals and Temporal Information to Control Rhythmic Expression of Genes Involved in Cell Fusion.
abstract: Light and the circadian clock have a profound effect on the biology of organisms through the regulation of large sets of genes. Toward understanding how light and the circadian clock regulate gene expression, we used genome-wide approaches to identify the direct and indirect targets of the light-responsive and clock-controlled transcription factor ADV-1 in Neurospora crassa A large proportion of ADV-1 targets were found to be light- and/or clock-controlled, and enriched for genes involved in development, metabolism, cell growth, and cell fusion. We show that ADV-1 is necessary for transducing light and/or temporal information to its immediate downstream targets, including controlling rhythms in genes critical to somatic cell fusion. However, while ADV-1 targets are altered in predictable ways in Δadv-1 cells in response to light, this is not always the case for rhythmic target gene expression. These data suggest that a complex regulatory network downstream of ADV-1 functions to generate distinct temporal dynamics of target gene expression relative to the central clock mechanism.
authors.affiliation: Department of Biology, Texas A&M University, College Station, Texas 77843.
authors.forename: Rigzin
authors.initials: R
authors.lastname: Dekhang
authors.affiliation: Department of Biology, Texas A&M University, College Station, Texas 77843.
authors.forename: Cheng
authors.identifier: 0000-0001-6282-4476
authors.initials: C
authors.lastname: Wu
authors.affiliation: Department of Biochemistry and Biophysics, Oregon State University, Corvallis, Oregon 97331.
authors.forename: Kristina M
authors.initials: KM
authors.lastname: Smith
authors.affiliation: Department of Biology, Texas A&M University, College Station, Texas 77843.
authors.forename: Teresa M
authors.initials: TM
authors.lastname: Lamb
authors.affiliation: Bioinformatics Program, Boston University, Massachusetts 02215.
authors.forename: Matthew
authors.initials: M
authors.lastname: Peterson
authors.affiliation: Department of Biochemistry and Biophysics, Oregon State University, Corvallis, Oregon 97331.
authors.forename: Erin L
authors.initials: EL
authors.lastname: Bredeweg
authors.affiliation: Department of Biology, Texas A&M University, College Station, Texas 77843.
authors.forename: Oneida
authors.initials: O
authors.lastname: Ibarra
authors.affiliation: Department of Genetics, Geisel School of Medicine at Dartmouth, Hanover, New Hampshire 03755.
authors.forename: Jillian M
authors.initials: JM
authors.lastname: Emerson
authors.affiliation: Department of Biology, Texas A&M University, College Station, Texas 77843.
authors.forename: Nirmala
authors.initials: N
authors.lastname: Karunarathna
authors.affiliation: Bioinformatics Program, Boston University, Massachusetts 02215.
authors.forename: Anna
authors.initials: A
authors.lastname: Lyubetskaya
authors.affiliation: Bioinformatics Program, Boston University, Massachusetts 02215.
authors.forename: Elham
authors.initials: E
authors.lastname: Azizi
authors.affiliation: Department of Biological Sciences, Rensselaer Polytechnic Institute, Troy, New York 12180.
authors.forename: Jennifer M
authors.initials: JM
authors.lastname: Hurley
authors.affiliation: Department of Genetics, Geisel School of Medicine at Dartmouth, Hanover, New Hampshire 03755.
authors.forename: Jay C
authors.initials: JC
authors.lastname: Dunlap
authors.affiliation: Bioinformatics Program, Boston University, Massachusetts 02215.|National Emerging Infectious Diseases Laboratories, Boston University, Massachusetts 02118.|Department of Microbiology, Boston University, Massachusetts 02215.|Department of Biomedical Engineering, Boston University, Massachusetts 02215.
authors.forename: James E
authors.initials: JE
authors.lastname: Galagan
authors.affiliation: Department of Biochemistry and Biophysics, Oregon State University, Corvallis, Oregon 97331.
authors.forename: Michael
authors.initials: M
authors.lastname: Freitag
authors.affiliation: Department of Biology, Texas A&M University, College Station, Texas 77843.
authors.forename: Matthew S
authors.initials: MS
authors.lastname: Sachs
authors.affiliation: Department of Biology, Texas A&M University, College Station, Texas 77843 dpedersen@bio.tamu.edu.
authors.forename: Deborah
authors.identifier: 0000-0002-1639-8215
authors.initials: D
authors.lastname: Bell-Pedersen
mesh_terms: D057906:Circadian Clocks / Q000235:genetics* / Q000502:physiology; D002940:Circadian Rhythm / Q000235:genetics; D005656:Fungal Proteins / Q000235:genetics; D015966:Gene Expression Regulation, Fungal; D053263:Gene Regulatory Networks / Q000235:genetics*; D016681:Genome, Fungal; D008027:Light; D009492:Neurospora crassa / Q000235:genetics* / Q000502:physiology; D014157:Transcription Factors / Q000235:genetics*
chemical_list: D005656:Fungal Proteins; D014157:Transcription Factors
keywords: circadian clock; development; phototransduction; regulatory network