title: Comparison between Manual and Automated Methods for Ki-67 Immunoexpression Quantification in Ameloblastomas.
abstract: Ameloblastoma is a common and unpredictable odontogenic tumor with high relapse rates. Several studies assessing the proliferative capacity of these neoplasms have been published, mainly using the protein Ki-67. Cell counts must be completed to determine the cell proliferation rate. Multiple methods have been developed for this purpose. The most widely used method is the labeling index, which has undergone changes over time to better facilitate cell counting. Here, we compared manual cell counting methods with automated cell counting (ImmunoRatio) to determine the relative effectiveness of these methods. The results suggest that ImmunoRatio, a free software tool, may be highly advantageous and provide results similar to manual cell counting methods when used with the appropriate calibration. However, ImmunoRatio has flaws that may affect the labeling index results. Therefore, this automated cell counting method must be supplemented with manual cell counting methods.
authors.affiliation: Department of Research, School of Dentistry, Universidad Juárez del Estado de Durango, Durango, DGO, Mexico.
authors.forename: Rogelio
authors.initials: R
authors.lastname: González-González
authors.affiliation: Department of Health Care, Universidad Autónoma Metropolitana, Unidad Xochimilco, Mexico City, Mexico.
authors.forename: Nelly
authors.identifier: 0000-0002-0435-053X
authors.initials: N
authors.lastname: Molina-Frechero
authors.affiliation: Department of Research, School of Dentistry, Universidad Juárez del Estado de Durango, Durango, DGO, Mexico.
authors.forename: Ramón G
authors.identifier: 0000-0002-4637-0782
authors.initials: RG
authors.lastname: Carreón-Burciaga
authors.affiliation: Microbiology and Pathology Department, Dentistry Research Institute, Health Sciences University Center, Universidad de Guadalajara, Guadalajara, JAL, Mexico.
authors.forename: Sandra
authors.initials: S
authors.lastname: López-Verdín
authors.affiliation: School of Health Sciences, Universidad Anahuac Norte, Lomas Anáhuac, Huixquilucan, MEX, Mexico.
authors.forename: Carlos
authors.initials: C
authors.lastname: Robles-Bonilla
authors.affiliation: Department of Molecular Pathology, School of Dentistry, Universidad de la República, Uruguay, Montevideo, Uruguay.
authors.forename: Vanesa
authors.initials: V
authors.lastname: Pereira-Prado
authors.affiliation: School of Health Sciences, Universidad Anahuac Norte, Lomas Anáhuac, Huixquilucan, MEX, Mexico; Department of Molecular Pathology, School of Dentistry, Universidad de la República, Uruguay, Montevideo, Uruguay.
authors.forename: Ronell
authors.identifier: 0000-0001-9755-4779
authors.initials: R
authors.lastname: Bologna-Molina
mesh_terms: D000564:Ameloblastoma / Q000276:immunology* / Q000473:pathology; D001331:Automation; D002452:Cell Count / Q000379:methods*; D006801:Humans; D019394:Ki-67 Antigen / Q000276:immunology*; D013194:Staining and Labeling
chemical_list: D019394:Ki-67 Antigen