title: PLD4 promotes M1 macrophages to perform antitumor effects in colon cancer cells.
abstract: Phospholipase D4 (PLD4) is a newly identified protein expressed in microglia. However, the function of PLD4 in tumor-associated macrophages (TAMs) is unknown. In the present study, we revealed that the expression of PLD4 was located in macrophages in the colon cancer mesenchymal and lymph nodes as shown by immunohistochemical analysis. furthermore, its expression was associated with clinical staging of colon cancer. Then, THP-1 as a cell model induced into TAMs. Western blot and RT-PCR analysis showed that PLD4 was mainly presented in M1 phenotype TAMs. The secretion of pro-inflammatory cytokines in M1 macrophages was significantly reduced after the expression of PLD4 inhibited by PLD4-siRNA. Furthermore, co-cultured with condition-medium from control or PLD4-siRNA M1 macrophages for 24 h, cell apoptosis, cycle and proliferation of cancer cells improved compared to control. These results indicated that PLD4 could be involved in the activation process of M1 phenotype macrophages.
authors.affiliation: The Gastroenterology Tumor and Microenvironment Laboratory, Department of Gastroenterology, The First Affiliated Hospital of Chengdu Medical College, Chengdu Medical College, Chengdu, Sichuan 610041, P.R. China.
authors.forename: Long
authors.initials: L
authors.lastname: Gao
authors.affiliation: The Gastroenterology Tumor and Microenvironment Laboratory, Department of Gastroenterology, The First Affiliated Hospital of Chengdu Medical College, Chengdu Medical College, Chengdu, Sichuan 610041, P.R. China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: The Gastroenterology Tumor and Microenvironment Laboratory, Department of Gastroenterology, The First Affiliated Hospital of Chengdu Medical College, Chengdu Medical College, Chengdu, Sichuan 610041, P.R. China.
authors.forename: Shu-Xian
authors.initials: SX
authors.lastname: Zhou
authors.affiliation: The Gastroenterology Tumor and Microenvironment Laboratory, Department of Gastroenterology, The First Affiliated Hospital of Chengdu Medical College, Chengdu Medical College, Chengdu, Sichuan 610041, P.R. China.
authors.forename: Xian-Jing
authors.initials: XJ
authors.lastname: Yu
authors.affiliation: The Gastroenterology Tumor and Microenvironment Laboratory, Department of Gastroenterology, The First Affiliated Hospital of Chengdu Medical College, Chengdu Medical College, Chengdu, Sichuan 610041, P.R. China.
authors.forename: Jin-Mei
authors.initials: JM
authors.lastname: Xu
authors.affiliation: The Gastroenterology Tumor and Microenvironment Laboratory, Department of Gastroenterology, The First Affiliated Hospital of Chengdu Medical College, Chengdu Medical College, Chengdu, Sichuan 610041, P.R. China.
authors.forename: Luo
authors.initials: L
authors.lastname: Zuo
authors.affiliation: Burn and Plastic Surgery Department, The First Affiliated Hospital of Chengdu Medical College, Chengdu, Sichuan 610500, P.R. China.
authors.forename: Yong-Hui
authors.initials: YH
authors.lastname: Luo
authors.affiliation: The Gastroenterology Tumor and Microenvironment Laboratory, Department of Gastroenterology, The First Affiliated Hospital of Chengdu Medical College, Chengdu Medical College, Chengdu, Sichuan 610041, P.R. China.
authors.forename: Xiao-An
authors.initials: XA
authors.lastname: Li
mesh_terms: D045744:Cell Line, Tumor; D018920:Coculture Techniques; D003110:Colonic Neoplasms / Q000378:metabolism / Q000473:pathology*; D017077:Culture Media, Conditioned / Q000494:pharmacology; D016207:Cytokines / Q000378:metabolism; D005092:Exonucleases; D006801:Humans; D008198:Lymph Nodes / Q000201:enzymology / Q000473:pathology; D008262:Macrophage Activation / Q000235:genetics; D008264:Macrophages / Q000502:physiology*; D010739:Phospholipase D / Q000235:genetics / Q000378:metabolism*; D034741:RNA, Small Interfering; D005090:Exodeoxyribonucleases
chemical_list: D017077:Culture Media, Conditioned; D016207:Cytokines; D005092:Exonucleases; D010739:Phospholipase D; D034741:RNA, Small Interfering; C579637:PLD4 protein, human; D005090:Exodeoxyribonucleases