title: Progesterone Receptor Membrane Component 1 Mediates Progesterone-Induced Suppression of Oocyte Meiotic Prophase I and Primordial Folliculogenesis.
abstract: Well-timed progression of primordial folliculogenesis is essential for mammalian female fertility. Progesterone (P4) inhibits primordial follicle formation under physiological conditions; however, P4 receptor that mediates this effect and its underlying mechanisms are unclear. In this study, we used an in vitro organ culture system to show that progesterone receptor membrane component 1 (PGRMC1) mediated P4-induced inhibition of oocyte meiotic prophase I and primordial follicle formation. We found that membrane-impermeable BSA-conjugated P4 inhibited primordial follicle formation similar to that by P4. Interestingly, PGRMC1 and its partner serpine1 mRNA-binding protein 1 were highly expressed in oocytes in perinatal ovaries. Inhibition or RNA interference of PGRMC1 abolished the suppressive effect of P4 on follicle formation. Furthermore, P4-PGRMC1 interaction blocked oocyte meiotic progression and decreased intra-oocyte cyclic AMP (cAMP) levels in perinatal ovaries. cAMP analog dibutyryl cAMP reversed P4-PGRMC1 interaction-induced inhibition of meiotic progression and follicle formation. Thus, our results indicated that PGRMC1 mediated P4-induced suppression of oocyte meiotic progression and primordial folliculogenesis by decreasing intra-oocyte cAMP levels.
authors.affiliation: Department of Laboratory Animal Science, School of Basic Medical Science, Capital Medical University, Beijing 100069, Peoples' Republic of China.
authors.forename: Meng
authors.initials: M
authors.lastname: Guo
authors.affiliation: College of Life Science, Capital Normal University, Beijing 100048, People's Republic of China.
authors.forename: Cheng
authors.initials: C
authors.lastname: Zhang
authors.affiliation: Department of Medical Genetics and Developmental Biology, School of Basic Medical Science, Capital Medical University, Beijing 100069, Peoples' Republic of China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Wang
authors.affiliation: State Key Laboratory for Agro-Biotechnology, College of Biological Science, China Agricultural University, Beijing 100193, Peoples' Republic of China.
authors.forename: Lizhao
authors.initials: L
authors.lastname: Feng
authors.affiliation: Laboratory of Cellular and Development Biology, NIDDK, National Institutes of Health, Bethesda MD 20892, USA.
authors.forename: Zhengpin
authors.initials: Z
authors.lastname: Wang
authors.affiliation: State Key Laboratory for Agro-Biotechnology, College of Biological Science, China Agricultural University, Beijing 100193, Peoples' Republic of China.
authors.forename: Wanbo
authors.initials: W
authors.lastname: Niu
authors.affiliation: Department of Laboratory Animal Science, School of Basic Medical Science, Capital Medical University, Beijing 100069, Peoples' Republic of China.
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Du
authors.affiliation: Department of Medical Genetics and Developmental Biology, School of Basic Medical Science, Capital Medical University, Beijing 100069, Peoples' Republic of China.
authors.forename: Wang
authors.initials: W
authors.lastname: Tang
authors.affiliation: Department of Medical Genetics and Developmental Biology, School of Basic Medical Science, Capital Medical University, Beijing 100069, Peoples' Republic of China.
authors.forename: Yuna
authors.initials: Y
authors.lastname: Li
authors.affiliation: State Key Laboratory for Agro-Biotechnology, College of Biological Science, China Agricultural University, Beijing 100193, Peoples' Republic of China.
authors.forename: Chao
authors.initials: C
authors.lastname: Wang
authors.affiliation: Department of Medical Genetics and Developmental Biology, School of Basic Medical Science, Capital Medical University, Beijing 100069, Peoples' Republic of China.
authors.forename: Zhenwen
authors.initials: Z
authors.lastname: Chen
mesh_terms: D000818:Animals; D000242:Cyclic AMP / Q000378:metabolism; D005260:Female; D020869:Gene Expression Profiling; D049469:Meiotic Prophase I / Q000187:drug effects*; D008565:Membrane Proteins / Q000378:metabolism*; D008813:Mice, Inbred ICR; D009865:Oocytes / Q000187:drug effects* / Q000502:physiology*; D009924:Organ Culture Techniques; D006080:Ovarian Follicle / Q000187:drug effects*; D011374:Progesterone / Q000378:metabolism*; D016601:RNA-Binding Proteins / Q000096:biosynthesis; D011980:Receptors, Progesterone / Q000378:metabolism*
chemical_list: D008565:Membrane Proteins; C508868:PGRMC1 protein, mouse; D016601:RNA-Binding Proteins; D011980:Receptors, Progesterone; C532831:SERBP1 protein, mouse; D011374:Progesterone; D000242:Cyclic AMP