title: Hepatic gene transcription profiles in turbot (Scophthalmus maximus) experimentally exposed to heavy fuel oil nº 6 and to styrene.
abstract: Oil and chemical spills in the marine environment, although sporadic, are highly dangerous to biota inhabiting coastal and estuarine areas. Effects of spilled compounds in exposed organisms occur at different biological organization levels: from molecular, cellular or tissue levels to the physiological one. The present study aims to determine the specific hepatic gene transcription profiles observed in turbot juveniles under exposure to fuel oil n °6 and styrene vs controls using an immune enriched turbot (Scophthalmus maximus) oligo-microarray containing 2716 specific gene probes. After 3 days of exposure, fuel oil specifically induced aryl hydrocarbon receptor mediated transcriptional response through up-regulation of genes, such as ahrr and cyp1a1. More gene transcripts were regulated after 14 days of exposure involved in ribosomal biosynthesis, immune modulation, and oxidative response among the most significantly regulated functional pathways. On the contrary, gene transcription alterations caused by styrene did not highlight any significantly regulated molecular or metabolic pathway. This was also previously reported at cell and tissue level where no apparent responses were distinguishable. For the fuel oil experiment, obtained specific gene profiles could be related to changes in cell-tissue organization in the same individuals, such as increased hepatocyte vacuolization, decrease in melano-macrophage centers and the regulation of leukocyte numbers. In conclusion, the mode of action reflected by gene transcription profiles analyzed hereby in turbot livers could be linked with the responses previously reported at higher biological organization levels. Molecular alterations described hereby could be preceding observed alterations at cell and tissue levels.
authors.affiliation: CBET Research Group, Dept. of Zoology and Animal Cell Biology, Fac. Science and Technology and Research Centre for Experimental Marine Biology and Biotechnology (PiE-UPV/EHU), University of the Basque Country (UPV/EHU), E-48080 Bilbao, PO Box 644, Basque Country, Spain.
authors.forename: Oihane
authors.initials: O
authors.lastname: Diaz de Cerio
authors.affiliation: CBET Research Group, Dept. of Zoology and Animal Cell Biology, Fac. Science and Technology and Research Centre for Experimental Marine Biology and Biotechnology (PiE-UPV/EHU), University of the Basque Country (UPV/EHU), E-48080 Bilbao, PO Box 644, Basque Country, Spain.
authors.forename: Eider
authors.initials: E
authors.lastname: Bilbao
authors.affiliation: CBET Research Group, Dept. of Zoology and Animal Cell Biology, Fac. Science and Technology and Research Centre for Experimental Marine Biology and Biotechnology (PiE-UPV/EHU), University of the Basque Country (UPV/EHU), E-48080 Bilbao, PO Box 644, Basque Country, Spain.
authors.forename: Pamela
authors.initials: P
authors.lastname: Ruiz
authors.affiliation: Departamento de Xenética, Facultade de Veterinaria, Universidade de Santiago de Compostela, Lugo, 27002, Spain.
authors.forename: Belén G
authors.initials: BG
authors.lastname: Pardo
authors.affiliation: Departamento de Xenética, Facultade de Veterinaria, Universidade de Santiago de Compostela, Lugo, 27002, Spain.
authors.forename: Paulino
authors.initials: P
authors.lastname: Martínez
authors.affiliation: CBET Research Group, Dept. of Zoology and Animal Cell Biology, Fac. Science and Technology and Research Centre for Experimental Marine Biology and Biotechnology (PiE-UPV/EHU), University of the Basque Country (UPV/EHU), E-48080 Bilbao, PO Box 644, Basque Country, Spain.
authors.forename: Miren P
authors.initials: MP
authors.lastname: Cajaraville
authors.affiliation: CBET Research Group, Dept. of Zoology and Animal Cell Biology, Fac. Science and Technology and Research Centre for Experimental Marine Biology and Biotechnology (PiE-UPV/EHU), University of the Basque Country (UPV/EHU), E-48080 Bilbao, PO Box 644, Basque Country, Spain. Electronic address: ibon.cancio@ehu.eus.
authors.forename: Ibon
authors.initials: I
authors.lastname: Cancio
mesh_terms: D000818:Animals; D019363:Cytochrome P-450 CYP1A1 / Q000235:genetics / Q000378:metabolism; D005412:Flatfishes / Q000502:physiology*; D005648:Fuel Oils / Q000633:toxicity*; D008099:Liver / Q000378:metabolism*; D018336:Receptors, Aryl Hydrocarbon / Q000235:genetics; D020058:Styrene / Q000633:toxicity*; D014158:Transcription, Genetic / Q000187:drug effects*; D014874:Water Pollutants, Chemical / Q000633:toxicity*
chemical_list: D005648:Fuel Oils; D018336:Receptors, Aryl Hydrocarbon; D014874:Water Pollutants, Chemical; D020058:Styrene; D019363:Cytochrome P-450 CYP1A1
keywords: Fuel oil; Liver; Microarray; Styrene; Transcriptomic; Turbot