title: Neutrophil plasticity enables the development of pathological microenvironments: implications for cystic fibrosis airway disease.
abstract: BACKGROUND
  The pathological course of several chronic inflammatory diseases, including cystic fibrosis, chronic obstructive pulmonary disease, and rheumatoid arthritis, features an aberrant innate immune response dominated by neutrophils. In cystic fibrosis, neutrophil burden and activity of neutrophil elastase in the extracellular fluid have been identified as strong predictors of lung disease severity.
  
  
  METHODS
  Although neutrophils are generally considered to be rigid, pre-programmed effector leukocytes, recent studies suggest extensive plasticity in how neutrophil functions unfold upon recruitment to peripheral tissues, and how they choose their ultimate fate. Indeed, upon migration to cystic fibrosis airways, neutrophils display dysregulated lifespan, metabolic activation, and altered effector and regulatory functions, consistent with profound adaptation and phenotypic reprogramming. Licensed by signals present in cystic fibrosis airway microenvironment to survive and develop these novel functions, neutrophils orchestrate, in partnership with the epithelium and with the resident microbiota, the evolution of a pathological microenvironment. This microenvironment is defined by altered proteolytic, redox, and metabolic balance and the presence of stable luminal structures in which neutrophils and microbes coexist.
  
  
  CONCLUSIONS
  The elucidation of molecular mechanisms driving neutrophil plasticity in vivo will open new treatment opportunities designed to modulate, rather than block, the crucial adaptive functions fulfilled by neutrophils. This review aims to outline emerging mechanisms of neutrophil plasticity and their participation in the building of pathological microenvironments in the context of cystic fibrosis and other diseases with similar features.
authors.affiliation: Department of Pediatrics, Emory University School of Medicine, Atlanta, GA, 30322, USA.|Center for CF and Airways Disease Research, Children's Healthcare of Atlanta, Atlanta, GA, 30322, USA.|Emory + Children's Center, 2015 Uppergate Dr NE, Rm 344, Atlanta, GA, 30322-1014, USA.
authors.forename: Camilla
authors.initials: C
authors.lastname: Margaroli
authors.affiliation: Department of Pediatrics, Emory University School of Medicine, Atlanta, GA, 30322, USA. tirouvanziam@emory.edu.|Center for CF and Airways Disease Research, Children's Healthcare of Atlanta, Atlanta, GA, 30322, USA. tirouvanziam@emory.edu.|Emory + Children's Center, 2015 Uppergate Dr NE, Rm 344, Atlanta, GA, 30322-1014, USA. tirouvanziam@emory.edu.
authors.forename: Rabindra
authors.identifier: http://orcid.org/0000-0003-3526-9985
authors.initials: R
authors.lastname: Tirouvanziam
keywords: Amino acids; Exocytosis; Glucose; Immunometabolism; Inflammation; Reprogramming