title: Effects of different photoperiod and trophic conditions on biomass, protein and lipid production by the marine alga Nannochloropsis gaditana at optimal concentration of desalination concentrate.
abstract: This study investigated the cultivation of the marine alga Nannochloropsis gaditana in a medium based on desalination concentrate (DC) with an optimal concentration of 75% DC, under three trophic conditions and four photoperiod schedules. N. gaditana produced a peak biomass concentration (1.25gL-1) under mixotrophic culture condition and a photoperiod of 16L:08D. N. gaditana cells compensate to different light-dark regimes producing different amounts of protein (17.9-44.8%). The intracellular lipid content in N. gaditana cells increased both under autotrophic conditions with a 16L:08D cycle (16.7%), and under mixotrophic conditions with a 08L:16D cycle (15.7%). In heterotrophic culture, N. gaditana cells were rich in polyunsaturated fatty acids (46.0%). This study demonstrates an alternative approach to enhancing intracellular lipid content of the marine alga N. gaditana by modifying the photoperiod, trophic conditions and stress-salinity-conductivity with the use of a DC-based medium.
authors.affiliation: Department of Food Science and Technology, Federal University of Santa Catarina, Av. Admar Gonzaga 1346, Itacorubi, 88034-001 Florianópolis, SC, Brazil. Electronic address: matos.a@posgrad.ufsc.br.
authors.forename: Ângelo Paggi
authors.initials: ÂP
authors.lastname: Matos
authors.affiliation: Department of Food Science and Technology, Federal University of Santa Catarina, Av. Admar Gonzaga 1346, Itacorubi, 88034-001 Florianópolis, SC, Brazil.
authors.forename: Monnik Gandin
authors.initials: MG
authors.lastname: Cavanholi
authors.affiliation: Department of Food Science and Technology, Federal University of Santa Catarina, Av. Admar Gonzaga 1346, Itacorubi, 88034-001 Florianópolis, SC, Brazil; Laboratory of Environmental Engineering, Southern University of Santa Catarina, Av. Pedra Branca, Unidade Pedra Branca, 88137-270, Palhoça, SC, Brazil.
authors.forename: Elisa Helena Siegel
authors.initials: EHS
authors.lastname: Moecke
authors.affiliation: Department of Food Science and Technology, Federal University of Santa Catarina, Av. Admar Gonzaga 1346, Itacorubi, 88034-001 Florianópolis, SC, Brazil.
authors.forename: Ernani Sebastião
authors.initials: ES
authors.lastname: Sant'Anna
mesh_terms: D052818:Autotrophic Processes; D018533:Biomass; D057486:Carbon Cycle; D052836:Heterotrophic Processes; D008027:Light; D008055:Lipids / Q000096:biosynthesis*; D017440:Photoperiod; D011506:Proteins / Q000378:metabolism; D058009:Stramenopiles / Q000378:metabolism*
chemical_list: D008055:Lipids; D011506:Proteins
keywords: Inland desalination wastewater; Lipid enhancement; Microalgae; Photo-osmotic; Saturated fatty acids