title: Synergistic Effect of Sarocladium sp. and Cryptococcus sp. Co-Culture on Crude Oil Biodegradation and Biosurfactant Production.
abstract: This study was conducted to evaluate the co-culture ability of two yeast (Sarocladium sp. and Cryptococcus sp.) isolates as compared to their individual cultures in surfactant production and oil degradation. The results showed that individual culture of each strain was capable of producing surfactant, degrading oil, and pyrene; also, a synergistic effect was observed when a co-culture was applied. Oil removal and biomass production were 28 and 35% higher in the co-culture than in individual cultures, respectively. To investigate the synergistic effects of mix culture on oil degradation, the surface tension, emulsification activity (EA), and cell surface hydrophobicity of individual and co-culture were studied. A comparison between the produced biosurfactant and chemical surfactants showed that individual culture of each yeast strain could reduce the surface tension like SDS and about 10% better than Tween 80. The results showed that the microbial consortium could reduce the surface tension more, by 10 and 20%, than SDS and Tween 80, respectively. Both individual cultures of Sarocladium sp. and Cryptococcus sp. showed good emulsification activity (0.329 and 0.412, respectively) when compared with a non-inoculated medium. Emulsification activity measurement for the two yeast mix cultures showed an excellent 33 and 67% increase as compared to the individual culture of Sarocladium sp. and Cryptococcus sp., respectively. The cell surface hydrophobicity of Sarocladium sp. and Cryptococcus sp. increased (38 and 85%) when the cells were treated with pyrene as a hydrophobic substrate for four generations. Finally, a 40% increase for pyrene degradation was measured in a co-culture of the two yeast mix culture. According to the results of the present study, the co-culture system exhibited better performance and this study will enhance the understanding of the synergistic effects of yeast co-culture on oil degradation.
authors.affiliation: Department of Microbial Biotechnology, School of Biology, College of Science, University of Tehran, Tehran, Iran.
authors.forename: Aliyeh
authors.initials: A
authors.lastname: Kamyabi
authors.affiliation: Department of Microbial Biotechnology, School of Biology, College of Science, University of Tehran, Tehran, Iran.
authors.forename: Hoda
authors.initials: H
authors.lastname: Nouri
authors.affiliation: Department of Microbial Biotechnology, School of Biology, College of Science, University of Tehran, Tehran, Iran. hmoghimi@ut.ac.ir.
authors.forename: Hamid
authors.initials: H
authors.lastname: Moghimi
mesh_terms: D001673:Biodegradation, Environmental; D018920:Coculture Techniques; D003454:Cryptococcus / Q000378:metabolism*; D004785:Environmental Pollutants / Q000378:metabolism*; D057927:Hydrophobic and Hydrophilic Interactions; D007700:Kinetics; D010578:Petroleum / Q000378:metabolism*; D011136:Polysorbates / Q000737:chemistry; D011721:Pyrenes / Q000378:metabolism*; D004718:Saccharomycetales / Q000378:metabolism*; D012967:Sodium Dodecyl Sulfate / Q000737:chemistry; D013500:Surface Tension; D013501:Surface-Active Agents / Q000378:metabolism
chemical_list: D004785:Environmental Pollutants; D010578:Petroleum; D011136:Polysorbates; D011721:Pyrenes; D013501:Surface-Active Agents; D012967:Sodium Dodecyl Sulfate; C030984:pyrene
keywords: Biosurfactant; Co-culture; Crude oil; Oil biodegradation; Pyrene