title: Metabolomics Predicts Neuroimaging Characteristics of Transient Ischemic Attack Patients.
abstract: BACKGROUND
  Neuroimaging is essential for the diagnosis and prognosis of transient ischemic attack (TIA). The discovery of a plasmatic biomarker related to neuroimaging findings is of enormous interest because, despite its relevance, magnetic resonance diffusion weighted imaging (DWI) is not always available in all hospitals that attend to TIA patients.
  
  
  METHODS
  Metabolomic analyses were performed by liquid chromatography coupled to mass spectrometry in order to establish the metabolomic patterns of positive DWI, DWI patterns and acute ischemic lesion volumes. We used these methods with an initial TIA cohort of 129 patients and validated them with a 2nd independent cohort of 152 patients.
  
  
  RESULTS
  Positive DWI was observed in 115 (40.9%) subjects and scattered pearls in one arterial territory was the most frequent lesion pattern (35.7%). The median acute ischemic lesion volume was 0.33 (0.15-1.90)cm3. We detected a specific metabolomic profile common to both cohorts for positive DWI (11 molecules including creatinine, threoninyl-threonine, N-acetyl-glucosamine, lyso phosphatidic acid and cholesterol-related molecules) and ischemic lesion volume (10 molecules including lysophosphatidylcholine, hypoxanthine/threonate, and leucines). Moreover lysophospholipids and creatinine clearly differed the subcortical DWI pattern from other patterns.
  
  
  CONCLUSIONS
  There are specific metabolomic profiles associated with representative neuroimaging features in TIA patients. Our findings could allow the development of serum biomarkers related to acute ischemic lesions and specific acute ischemic patterns.
authors.affiliation: Stroke Unit, Department of Neurology, Universitat de Lleida, Hospital Universitari Arnau de Vilanova de Leida, Clinical Neurosciences Group IRBLleida, Spain. Electronic address: fpurroygarcia@gmail.com.
authors.forename: Francisco
authors.initials: F
authors.lastname: Purroy
authors.affiliation: Stroke Unit, Department of Neurology, Universitat de Lleida, Hospital Universitari Arnau de Vilanova de Leida, Clinical Neurosciences Group IRBLleida, Spain.
authors.forename: Serafi
authors.initials: S
authors.lastname: Cambray
authors.affiliation: Stroke Unit, Department of Neurology, Universitat de Lleida, Hospital Universitari Arnau de Vilanova de Leida, Clinical Neurosciences Group IRBLleida, Spain.
authors.forename: Gerard
authors.initials: G
authors.lastname: Mauri-Capdevila
authors.affiliation: NUTREN-Nutrigenomics Center, Department of Experimental Medicine, Parc Científic i Tecnològic Agroalimentari de Lleida-Universitat de Lleida-IRBLLEIDA, Lleida, Spain.
authors.forename: Mariona
authors.initials: M
authors.lastname: Jové
authors.affiliation: Stroke Unit, Department of Neurology, Universitat de Lleida, Hospital Universitari Arnau de Vilanova de Leida, Clinical Neurosciences Group IRBLleida, Spain.
authors.forename: Jordi
authors.initials: J
authors.lastname: Sanahuja
authors.affiliation: Laboratori Clinic, Universitari Arnau de Vilanova de Lleida, Clinical Neurosciences Group IRBLleida, Spain.
authors.forename: Joan
authors.initials: J
authors.lastname: Farré
authors.affiliation: Stroke Unit, Department of Neurology, Universitat de Lleida, Hospital Universitari Arnau de Vilanova de Leida, Clinical Neurosciences Group IRBLleida, Spain.
authors.forename: Ikram
authors.initials: I
authors.lastname: Benabdelhak
authors.affiliation: Stroke Unit, Department of Neurology, Universitat de Lleida, Hospital Universitari Arnau de Vilanova de Leida, Clinical Neurosciences Group IRBLleida, Spain.
authors.forename: Jessica
authors.initials: J
authors.lastname: Molina-Seguin
authors.affiliation: Stroke Unit, Department of Neurology, Universitat de Lleida, Hospital Universitari Arnau de Vilanova de Leida, Clinical Neurosciences Group IRBLleida, Spain.
authors.forename: Laura
authors.initials: L
authors.lastname: Colàs-Campàs
authors.affiliation: Stroke Unit, Department of Neurology, Universitat de Lleida, Hospital Universitari Arnau de Vilanova de Leida, Clinical Neurosciences Group IRBLleida, Spain.
authors.forename: Robert
authors.initials: R
authors.lastname: Begue
authors.affiliation: Stroke Unit, Department of Neurology, Universitat de Lleida, Hospital Universitari Arnau de Vilanova de Leida, Clinical Neurosciences Group IRBLleida, Spain.
authors.forename: M Isabel
authors.initials: MI
authors.lastname: Gil
authors.affiliation: NUTREN-Nutrigenomics Center, Department of Experimental Medicine, Parc Científic i Tecnològic Agroalimentari de Lleida-Universitat de Lleida-IRBLLEIDA, Lleida, Spain.
authors.forename: Reinald
authors.initials: R
authors.lastname: Pamplona
authors.affiliation: NUTREN-Nutrigenomics Center, Department of Experimental Medicine, Parc Científic i Tecnològic Agroalimentari de Lleida-Universitat de Lleida-IRBLLEIDA, Lleida, Spain.
authors.forename: Manuel
authors.initials: M
authors.lastname: Portero-Otín
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D015415:Biomarkers; D001921:Brain / Q000378:metabolism* / Q000473:pathology*; D016000:Cluster Analysis; D038524:Diffusion Magnetic Resonance Imaging; D005260:Female; D006801:Humans; D002546:Ischemic Attack, Transient / Q000000981:diagnostic imaging* / Q000188:drug therapy / Q000209:etiology / Q000378:metabolism*; D008297:Male; D055442:Metabolome; D055432:Metabolomics / Q000379:methods; D008875:Middle Aged; D059906:Neuroimaging / Q000379:methods; D011379:Prognosis; D012307:Risk Factors
chemical_list: D015415:Biomarkers
keywords: Diffusion magnetic resonance imaging; Metabolomics; TIA