title: Comparison between urine culture profile and morphology classification using fluorescence parameters of the Sysmex UF-1000i urine flow cytometer.
abstract: OBJECTIVE
  To determine the usefulness of the fluorescence parameters generated by Sysmex UF-1000i flow cytometer for the rapid diagnosis of urinary tract infection by bacilli or cocci.
  
  
  RESULTS
  Urine samples (n = 1924) were studied by culture and microbiology and subsequently by cytometry, using BACT-Morph software and considering forward-scattered light (FSC) and fluorescent light scatter fluorescence parameters. BACT-Morph software showed moderate diagnostic accuracy (78·4%) to detect rod-shaped bacteria, with sensitivity of 82·4% and specificity of 62·5%. Forward-scattered (B_FSC) values of the bacterial channel were significant higher for the Gram-positive cocci category (P < 0·001). A cut-off of B_FSC ≥24·2, expressed in arbitrary units (analytical channel, ch), provided higher sensitivity (90·0%) but lower specificity (38·9%), and the diagnostic accuracy for Gram-positive cocci classification reached 62·0%.
  
  
  CONCLUSIONS
  Utilization of BACT-Morph software and bacterial channel fluorescence parameters (B_FSC ≥24·2 ch) offered an approximate discrimination of bacilli and cocci but the specificity was low, especially for FSC.
  
  
  CONCLUSIONS
  Further research is needed to establish the usefulness of flow cytometry for aetiological diagnosis.
authors.affiliation: Laboratorio de Microbiología, Complejo Hospitalario Universitario de Granada (Hospital Virgen de las Nieves)-IBS, Granada, Spain.
authors.forename: G
authors.initials: G
authors.lastname: Jiménez-Guerra
authors.affiliation: Laboratorio de Microbiología, Complejo Hospitalario Universitario de Granada (Hospital Virgen de las Nieves)-IBS, Granada, Spain.
authors.forename: V
authors.initials: V
authors.lastname: Heras-Cañas
authors.affiliation: Laboratorio de Microbiología, Complejo Hospitalario Universitario de Granada (Hospital Virgen de las Nieves)-IBS, Granada, Spain.
authors.forename: M D
authors.initials: MD
authors.lastname: Valera-Arcas
authors.affiliation: Laboratorio de Microbiología, Complejo Hospitalario Universitario de Granada (Hospital Virgen de las Nieves)-IBS, Granada, Spain.
authors.forename: J
authors.initials: J
authors.lastname: Rodríguez-Grangér
authors.affiliation: Laboratorio de Microbiología, Complejo Hospitalario Universitario de Granada (Hospital Virgen de las Nieves)-IBS, Granada, Spain.
authors.forename: J M
authors.initials: JM
authors.lastname: Navarro
authors.affiliation: Laboratorio de Microbiología, Complejo Hospitalario Universitario de Granada (Hospital Virgen de las Nieves)-IBS, Granada, Spain.|Departamento de Microbiología, Universidad de Granada-IBS, Granada, Spain.
authors.forename: J
authors.initials: J
authors.lastname: Gutiérrez-Fernández
mesh_terms: D004396:Coloring Agents; D005260:Female; D005434:Flow Cytometry / Q000379:methods; D005453:Fluorescence; D006801:Humans; D006967:Hypersensitivity; D008027:Light; D008297:Male; D012680:Sensitivity and Specificity; D012984:Software; D016482:Urinalysis / Q000379:methods*; D014552:Urinary Tract Infections / Q000382:microbiology; D014556:Urine / Q000382:microbiology
chemical_list: D004396:Coloring Agents
keywords: diagnosis; fluorescence; urinary tract infection; urine culture; urine flow cytometer