title: Zanthoxylum bungeanum pericarp extract prevents dextran sulfate sodium-induced experimental colitis in mice via the regulation of TLR4 and TLR4-related signaling pathways.
abstract: Zanthoxylum bungeanum, which belongs to the Zanthoxylum genus of the Rutaceae family, is now wildly distributed in most parts of China and some Southeast Asian countries. The pericarp of Zanthoxylum bungeanum has been known to exhibit antibacterial, anti-inflammatory and other important therapeutic activities. The purpose of this study was to investigate the effects and mechanisms of Zanthoxylum bungeanum pericarp extract (ZBE) on DSS-induced experimental colitis in mice. The results demonstrated that the major flavonoid composition of ZBE includes rutin (32.36%), quercetin (13.61%) and isoquercitrin (24.89%). ZBE alleviated DSS-induced body weight loss, colon length shortening and colonic pathological damage. Furthermore, ZBE inhibited the expression of TNF-α, IL-1β and IL-12 via the regulation of TLR4 and TLR4-related pathways in DSS-induced experimental colitis in mice and LPS-triggered inflammation in J774.1 cells. Our findings suggest that ZBE is effective in ameliorating experimental colitis, and further investigation is necessary on the use of ZBE as a new dietary strategy to lower the risk of ulcerative colitis (UC).
authors.affiliation: College of Veterinary Medicine, Jilin University, Changchun 130062, People's Republic of China.
authors.forename: Zecai
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: College of Veterinary Medicine, Jilin University, Changchun 130062, People's Republic of China.
authors.forename: Jiuxi
authors.initials: J
authors.lastname: Liu
authors.affiliation: College of Veterinary Medicine, Jilin University, Changchun 130062, People's Republic of China.
authors.forename: Peng
authors.initials: P
authors.lastname: Shen
authors.affiliation: College of Veterinary Medicine, Jilin University, Changchun 130062, People's Republic of China.
authors.forename: Yongguo
authors.initials: Y
authors.lastname: Cao
authors.affiliation: College of Veterinary Medicine, Jilin University, Changchun 130062, People's Republic of China.
authors.forename: Xiaojie
authors.initials: X
authors.lastname: Lu
authors.affiliation: College of Veterinary Medicine, Jilin University, Changchun 130062, People's Republic of China.
authors.forename: Xuejiao
authors.initials: X
authors.lastname: Gao
authors.affiliation: College of Veterinary Medicine, Jilin University, Changchun 130062, People's Republic of China.
authors.forename: Yunhe
authors.initials: Y
authors.lastname: Fu
authors.affiliation: College of Veterinary Medicine, Jilin University, Changchun 130062, People's Republic of China. Electronic address: liuboy@hotmail.com.
authors.forename: Bo
authors.initials: B
authors.lastname: Liu
authors.affiliation: College of Veterinary Medicine, Jilin University, Changchun 130062, People's Republic of China. Electronic address: zhangns@jlu.edu.cn.
authors.forename: Naisheng
authors.initials: N
authors.lastname: Zhang
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology / Q000627:therapeutic use*; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D003093:Colitis, Ulcerative / Q000139:chemically induced / Q000188:drug therapy* / Q000276:immunology; D016207:Cytokines / Q000276:immunology; D016264:Dextran Sulfate; D004195:Disease Models, Animal; D008198:Lymph Nodes / Q000166:cytology; D008297:Male; D008810:Mice, Inbred C57BL; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology / Q000627:therapeutic use*; D015398:Signal Transduction / Q000187:drug effects; D051197:Toll-Like Receptor 4 / Q000276:immunology; D032106:Zanthoxylum
chemical_list: D000893:Anti-Inflammatory Agents; D016207:Cytokines; D010936:Plant Extracts; C493487:Tlr4 protein, mouse; D051197:Toll-Like Receptor 4; D016264:Dextran Sulfate
keywords: Colitis; Inflammation; J774.1 cells; Signaling pathway; Zanthoxylum bungeanum