title: Real-time monitoring of vesicle pH in an endocytic pathway using an EGF-conjugated two-photon probe.
abstract: Herein, we developed a ratiometric two-photon probe (BHS3-EGF), derived from a pH sensitive dye and epidermal growth factor (EGF), for real-time monitoring and quantitative analysis of acidic luminal pH values during endocytic pathway activity. Two-photon microscopy imaging with BHS3-EGF allows the quantitative analysis of pH distributions of single vesicles and their dynamics in receptor-mediated endocytosis in real-time.
authors.affiliation: Department of Energy Systems Research, Ajou University, Suwon 443-749, Korea. kimhm@ajou.ac.kr.
authors.forename: Cheol Ho
authors.initials: CH
authors.lastname: Heo
authors.affiliation: Department of Energy Systems Research, Ajou University, Suwon 443-749, Korea. kimhm@ajou.ac.kr.
authors.forename: Myoung Ki
authors.initials: MK
authors.lastname: Cho
authors.affiliation: Department of Molecular Science and Technology, Ajou University, Suwon 433-721, Korea. taehyeonyoo@ajou.ac.kr.
authors.forename: Seunggun
authors.initials: S
authors.lastname: Shin
authors.affiliation: Department of Molecular Science and Technology, Ajou University, Suwon 433-721, Korea. taehyeonyoo@ajou.ac.kr.
authors.forename: Tae Hyeon
authors.initials: TH
authors.lastname: Yoo
authors.affiliation: Department of Energy Systems Research, Ajou University, Suwon 443-749, Korea. kimhm@ajou.ac.kr.
authors.forename: Hwan Myung
authors.initials: HM
authors.lastname: Kim