title: A novel picorna-like virus, Riptortus pedestris virus-1 (RiPV-1), found in the bean bug, R. pedestris, after fungal infection.
abstract: A viral genome was assembled de novo from next-generation sequencing (NGS) data from bean bugs, Riptortus pedestris, infected with an entomopathogenic fungus, Beauveria bassiana (Bb), and was further confirmed via the RACE method. This is a novel insect positive-sense single-stranded RNA virus, which we named Riptortus pedestris virus-1 (RiPV-1) (GenBank accession no. KU958718). The genome of RiPV-1 consists of 10,554 nucleotides (nt), excluding the poly(A) tail, which contains a single large open reading frame (ORF) of 10,371 nt encoding a polyprotein (3456 aa) and flanked by 71 and 112 nt at the 5' and 3' untranslated regions (UTR), respectively. RiPV-1 genome organization from the 5' end contains a consensus organization of picorna-like RNA virus helicase, cysteine protease, and RNA-dependent RNA polymerase (RdRp), in addition to two putative structural proteins located at the 3' region and a poly(A) tail at the 3' end. The viral particles were approximately 30nm in diameter with some dispersal distinctive surface projections. Based on the phylogenetic analysis of the RdRp sequences, RiPV-1 was clustered in the unassigned insect RNA viruses with two other viruses, APV and KFV. These three viruses were suggested to constitute a new group of insect RNA viruses. RiPV-1 could be found in all stages of lab-reared bean bugs and was detected abundantly in the thorax, abdomen, midgut and fat body, but not in the reproductive organs and muscle. Interestingly, RiPV-1 replication was increased dramatically in bean bugs 2-6days after fungal infection. In conclusion, a novel insect RNA virus was found by NGS data assembly. This virus can provide further insight into the interaction between virus, fungus and the host.
authors.affiliation: Department of Agricultural Biology, College of Agriculture & Life Sciences, Chonbuk National University, Jeonju, Republic of Korea.
authors.forename: Yi-Ting
authors.initials: YT
authors.lastname: Yang
authors.affiliation: Department of Agricultural Biology, College of Agriculture & Life Sciences, Chonbuk National University, Jeonju, Republic of Korea; Department of Biotechnology and Animal Science, National Ilan University, Ilan, Taiwan.
authors.forename: Yu-Shin
authors.initials: YS
authors.lastname: Nai
authors.affiliation: Department of Agricultural Biology, College of Agriculture & Life Sciences, Chonbuk National University, Jeonju, Republic of Korea.
authors.forename: Se Jin
authors.initials: SJ
authors.lastname: Lee
authors.affiliation: Department of Agricultural Biology, College of Agriculture & Life Sciences, Chonbuk National University, Jeonju, Republic of Korea.
authors.forename: Mi Rong
authors.initials: MR
authors.lastname: Lee
authors.affiliation: Department of Agricultural Biology, College of Agriculture & Life Sciences, Chonbuk National University, Jeonju, Republic of Korea.
authors.forename: Sihyeon
authors.initials: S
authors.lastname: Kim
authors.affiliation: Department of Agricultural Biology, College of Agriculture & Life Sciences, Chonbuk National University, Jeonju, Republic of Korea. Electronic address: jskim10@jbnu.ac.kr.
authors.forename: Jae Su
authors.initials: JS
authors.lastname: Kim
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D052982:Beauveria; D060085:Coinfection; D016679:Genome, Viral; D020062:Heteroptera / Q000469:parasitology / Q000821:virology*; D059014:High-Throughput Nucleotide Sequencing; D007304:Insect Viruses / Q000235:genetics*; D009181:Mycoses / Q000662:veterinary; D010802:Phylogeny; D012328:RNA Viruses / Q000235:genetics*; D012367:RNA, Viral; D016415:Sequence Alignment
chemical_list: D012367:RNA, Viral
keywords: Beauveria bassiana; Picorna-like virus; RiPV-1; Riptortus pedestris