title: [Effect of different doses of radiation on human salivary gland adenoid cystic carcinoma cells].
abstract: OBJECTIVE
  To explore the effect of different doses of radiation on human salivary gland adenoid cystic carcinoma cells (SACC-83, SACC-LM).
  
  
  METHODS
  Different doses of radiation (0, 2, 4, 6, 8, 10 Gy) were applied to SACC-83, SACC-LM cells and the cells were continued to culture for 48 h. CCK-8 test, flow cytometry(FCM) and cell scratch experiment were used to observe cell proliferation, apoptosis and cell migration. SPSS19.0 software package was used for data analysis.
  
  
  RESULTS
  The effect of radiation on SACC-LM cells survival rate, cell apoptosis, heteroploid and cell migration ability were significantly greater than that on SACC-83 cells (P<0.05). In SACC-83 cells, compared with other doses of radiation, 6 Gy of irradiation was the most sensitive on cell survival rate, cell apoptosis, heteroploid and cell migration ability.
  
  
  CONCLUSIONS
  Radiation sensitivity of SACC-LM was greater than that of SACC-83 cells. With 6 Gy of radiation, changes of biology in SACC-83 cells most significant than other doses of radiation.
authors.affiliation: Department of Oral and Maxillofacial Surgery, Stomatological Hospital of Chongqing Medical University. Chongqing 401147,China. E-mail:huangymcq@163.com.
authors.forename: Yu-Meng
authors.initials: YM
authors.lastname: Huang
authors.forename: Ying
authors.initials: Y
authors.lastname: Wang
authors.forename: Yong
authors.initials: Y
authors.lastname: Li
authors.forename: Ping
authors.initials: P
authors.lastname: Liu
authors.forename: Xian-Feng
authors.initials: XF
authors.lastname: Liu
authors.forename: Hong-Wei
authors.initials: HW
authors.lastname: Zhao
mesh_terms: D017209:Apoptosis; D003528:Carcinoma, Adenoid Cystic / Q000532:radiotherapy*; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D006801:Humans; D012468:Salivary Gland Neoplasms / Q000532:radiotherapy*; D012469:Salivary Glands