title: A mechanistic model of ion-exchange chromatography on polymer fiber stationary phases.
abstract: Fibers are prominent among novel stationary phase supports for preparative chromatography. Several recent studies have highlighted the potential of fiber-based adsorbents for high productivity downstream processing in both batch and continuous mode, but so far the development of these materials and of processes employing these materials has solely been based on experimental data. In this study we assessed whether mechanistic modeling can be performed on fiber-based adsorbents. With a column randomly filled with short cut hydrogel grafted anion exchange fibers, we tested whether tracer, linear gradient elution, and breakthrough data could be reproduced by mechanistic models. Successful modeling was achieved for all of the considered experiments, for both non-retained and retained molecules. For the fibers used in this study the best results were obtained with a transport-dispersive model in combination with a steric mass action isotherm. This approach accurately accounted for the convection and dispersion of non-retained tracers, and the breakthrough and elution behaviors of three different proteins with sizes ranging from 6 to 160kDa were accurately modeled, with simulation results closely resembling the experimental data. The estimated model parameters were plausible both from their physical meaning, and from an analysis of the underlying model assumptions. Parameters were determined within good confidence levels; the average confidence estimate was below 7% for confidence levels of 95%. This shows that fiber-based adsorbents can be modeled mechanistically, which will be valuable for the future design and evaluation of these novel materials and for the development of processes employing such materials.
authors.affiliation: Institute of Engineering in Life Sciences, Section IV: Biomolecular Separation Engineering, Karlsruhe Institute of Technology (KIT), Karlsruhe, Germany. Electronic address: johannes.winderl@kit.edu.
authors.forename: Johannes
authors.initials: J
authors.lastname: Winderl
authors.affiliation: GoSilico GmbH, Karlsruhe, Germany. Electronic address: tobias.hahn@gosilico.com.
authors.forename: Tobias
authors.initials: T
authors.lastname: Hahn
authors.affiliation: Institute of Engineering in Life Sciences, Section IV: Biomolecular Separation Engineering, Karlsruhe Institute of Technology (KIT), Karlsruhe, Germany. Electronic address: juergen.hubbuch@kit.edu.
authors.forename: Jürgen
authors.initials: J
authors.lastname: Hubbuch
mesh_terms: D002852:Chromatography, Ion Exchange; D008956:Models, Chemical; D011108:Polymers / Q000737:chemistry; D011506:Proteins / Q000302:isolation & purification
chemical_list: D011108:Polymers; D011506:Proteins
keywords: Fibers; Ion exchange chromatography; Mechanistic modeling; Preparative chromatography; Proteins; Stationary phase