title: MHC class II peptides induce CD8+CD44+Ly49+regulatory T cells in C57BL/6 mice.
abstract: CD8+regulatory T cells (Tregs) play an important role in regulating peripheral immune tolerance. However, difficulties in the characterization of CD8+Tregs that lack suitable markers have a considerably limited research in this area. Moreover, the induction and effector mechanisms of CD8+Tregs remain unclear. Herein, we demonstrate the suitability of Ly49 and CD44 as markers for CD8+Tregs. Our data also show that MHC class II restricted peptides induce CD8+CD44+Ly49+Tregs via CD4+T cell activation. Furthermore, we also found cross-suppressive activity of these CD8+Tregs on responding CD4+T cells in a cytotoxicity dependent manner. Our data provide new insights into the induction and function of CD8+Tregs.
authors.affiliation: Program in Molecular & Translational Medicine, PMTM, Huzhou University, Huzhou 313000, China.
authors.forename: Yunliang
authors.initials: Y
authors.lastname: Yao
authors.affiliation: First Affiliated Hospital, Huzhou University, Huzhou 313000, China.
authors.forename: Xiang
authors.initials: X
authors.lastname: Wang
authors.affiliation: Program in Molecular & Translational Medicine, PMTM, Huzhou University, Huzhou 313000, China.
authors.forename: Hongchang
authors.initials: H
authors.lastname: Zhou
authors.affiliation: Program in Molecular & Translational Medicine, PMTM, Huzhou University, Huzhou 313000, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Program in Molecular & Translational Medicine, PMTM, Huzhou University, Huzhou 313000, China.
authors.forename: Yinfang
authors.initials: Y
authors.lastname: Shi
authors.affiliation: Program in Molecular & Translational Medicine, PMTM, Huzhou University, Huzhou 313000, China.
authors.forename: Shiyu
authors.initials: S
authors.lastname: Wang
authors.affiliation: First Affiliated Hospital, Huzhou University, Huzhou 313000, China. Electronic address: wangxy0308@hotmail.com.
authors.forename: Xiaoyi
authors.initials: X
authors.lastname: Wang
mesh_terms: D000217:Acyltransferases / Q000276:immunology / Q000378:metabolism; D000818:Animals; D000942:Antigens, Bacterial / Q000276:immunology / Q000378:metabolism; D001426:Bacterial Proteins / Q000276:immunology / Q000378:metabolism; D024201:Bystander Effect; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology*; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D002478:Cells, Cultured; D003602:Cytotoxicity, Immunologic; D005260:Female; D000949:Histocompatibility Antigens Class II / Q000378:metabolism; D018960:Hyaluronan Receptors / Q000378:metabolism; D007114:Immunization; D007165:Immunosuppression Therapy; D008213:Lymphocyte Activation; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D063308:Myelin-Oligodendrocyte Glycoprotein / Q000276:immunology / Q000378:metabolism; D055650:NK Cell Lectin-Like Receptor Subfamily A / Q000378:metabolism; D010446:Peptide Fragments / Q000276:immunology / Q000378:metabolism
chemical_list: D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D000949:Histocompatibility Antigens Class II; D018960:Hyaluronan Receptors; C474482:I-A(b) antigen, mouse; D063308:Myelin-Oligodendrocyte Glycoprotein; D055650:NK Cell Lectin-Like Receptor Subfamily A; D010446:Peptide Fragments; C445081:myelin oligodendrocyte glycoprotein (35-55); D000217:Acyltransferases; C121433:antigen 85B, Mycobacterium tuberculosis
keywords: CD8(+) T regulatory cells; Ly49; MHC class I; Peptides