title: Highly sensitive detection of cancer cells with an electrochemical cytosensor based on boronic acid functional polythiophene.
abstract: The detection of cancer cells through important molecular recognition target such as sialic acid is significant for the clinical diagnosis and treatment. There are many electrochemical cytosensors developed for cancer cells detection but most of them have complicated fabrication processes which results in poor reproducibility and reliability. In this study, a simple, low-cost, and highly sensitive electrochemical cytosensor was designed based on boronic acid-functionalized polythiophene. In cytosensors fabrication simple single-step procedure was used which includes coating pencil graphite electrode (PGE) by means of electro-polymerization of 3-Thienyl boronic acid and Thiophen. Electrochemical impedance spectroscopy and cyclic voltammetry were used as an analytical methods to optimize and measure analytical performances of PGE/P(TBA0.5Th0.5) based electrode. Cytosensor showed extremely good analytical performances in detection of cancer cells with linear rage of 1×101to 1×106cellsmL-1exhibiting low detection limit of 10 cellsmL-1and incubation time of 10min. Next to excellent analytical performances, it showed high selectivity towards AGS cancer cells when compared to HEK 293 normal cells and bone marrow mesenchymal stem cells (BM-hMSCs). This method is promising for future applications in early stage cancer diagnosis.
authors.affiliation: Biotechnology Research Lab, EMC Technology Inc, ARGEM Building, Technocity, Avcılar, Istanbul 34320, Turkey.
authors.forename: Muamer
authors.initials: M
authors.lastname: Dervisevic
authors.affiliation: Biotechnology Research Lab, EMC Technology Inc, ARGEM Building, Technocity, Avcılar, Istanbul 34320, Turkey. Electronic address: msenel81@gmail.com.
authors.forename: Mehmet
authors.initials: M
authors.lastname: Senel
authors.affiliation: Orta Mah. Sunay Sok. No 1, Kartal, Istanbul 34880, Turkey.
authors.forename: Tugba
authors.initials: T
authors.lastname: Sagir
authors.affiliation: Biotechnology Research Lab, EMC Technology Inc, ARGEM Building, Technocity, Avcılar, Istanbul 34320, Turkey.
authors.forename: Sevim
authors.initials: S
authors.lastname: Isik
mesh_terms: D015374:Biosensing Techniques; D001854:Bone Marrow Cells / Q000473:pathology; D001897:Boronic Acids / Q000737:chemistry; D002469:Cell Separation / Q000379:methods*; D058266:Dielectric Spectroscopy; D006046:Gold / Q000737:chemistry; D006108:Graphite; D057809:HEK293 Cells; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000473:pathology; D053768:Metal Nanoparticles; D009369:Neoplasms / Q000175:diagnosis* / Q000473:pathology; D011108:Polymers / Q000737:chemistry*; D013876:Thiophenes / Q000737:chemistry*
chemical_list: D001897:Boronic Acids; D011108:Polymers; D013876:Thiophenes; C066730:polythiophene; D006046:Gold; D006108:Graphite
keywords: Boronic acid; Cancer cells; Conducting polymer; Cytosensor; Electrochemical impedance; Sialic acid