title: Characterization of Prototype Foamy Virus Infectivity in Transportin 3 Knockdown Human 293t Cell Line.
abstract: The foamy viruses are currently considered essential for development as vectors for gene delivery. Previous studies demonstrated that prototype foamy virus (PFV) can infect and replicate prevalently in a variety of cell types for its exclusive replication strategy. However, the virus-host interaction, especially PFV-transportin3 (TNPO3), is still poorly understood. In our investigation of the role of TNPO3 in PFV infection, we found lower virus production in TNPO3 knockdown (KD) cells compared with wild-type 293T cells. PCR analysis revealed that viral DNAs were mostly altered to circular forms: both 1-long terminal repeat (1-LTR) and 2-LTR in TNPO3 KD cells. We therefore suggest that TNPO3 is required for successful PFV replication, at least at/after the nuclear entry step of viral DNA. These findings highlight the obscure mysteries of PFV-host interaction and the requirement of TNPO3 for productive infection of PFV in 293T cells.
authors.affiliation: Department of Systems Biotechnology, Chung-Ang University, Ansung 17546, Republic of Korea.
authors.forename: Faysal Bin
authors.initials: FB
authors.lastname: Hamid
authors.affiliation: Department of Systems Biotechnology, Chung-Ang University, Ansung 17546, Republic of Korea.
authors.forename: Jinsun
authors.initials: J
authors.lastname: Kim
authors.affiliation: Department of Systems Biotechnology, Chung-Ang University, Ansung 17546, Republic of Korea.
authors.forename: Cha-Gyun
authors.initials: CG
authors.lastname: Shin
mesh_terms: D004279:DNA, Viral; D055785:Gene Knockdown Techniques; D057809:HEK293 Cells; D054884:Host-Pathogen Interactions; D006801:Humans; D016092:Spumavirus / Q000235:genetics / Q000502:physiology*; D020079:Terminal Repeat Sequences; D014162:Transfection; D014779:Virus Replication; D028961:beta Karyopherins / Q000235:genetics / Q000502:physiology*
chemical_list: D004279:DNA, Viral; C546476:TNPO3 protein, human; D028961:beta Karyopherins
keywords: 1-long terminal repeat; 293 T cell; Transportin-3; prototype foamy virus