title: A straightforward method to produce decellularized dermis-based matrices for tumour cell cultures.
abstract: Decellularized matrices are steadily gaining popularity to study the biology of cells and tissues, as they represent a biomimetic environment in which cells can recapitulate certain behaviours that share similarities with those observed in vivo. Basically, biochemistry, microstructure and mechanics of the decellularized matrices are the most valuable properties that differentiate these culturing systems from conventional bidimensional models. Several procedures to decellularize tissues have been proposed so far, with the common aim to preserve the tissue chemical/physical properties of the original tissue. However, these processes are complex, time-consuming and expensive. In this work, we propose a cost-effective, easy-to-produce decellularized dermal matrix, derived from animal skin. The chemical/physical processes to obtain the matrices proved to not alter matrix structure and did not induce cytotoxicity issues. To test the validity of the decellularized matrices as a model to study the behaviour of tumour cells in vitro, we performed microstructural and mechanical investigations as well as cell proliferation assays. In particular, three different tumour cell lines were used, which proliferated and invaded the matrix with no additional treatments. Decellularized skin scaffold, presented in this work, could be a strong competitor for conventional 3D systems like synthetic porous scaffolds or hydrogels. Copyright © 2016 John Wiley & Sons, Ltd.
authors.affiliation: Interdisciplinary Research Centre on Biomaterials, University of Naples Federico II, P.le Tecchio 80, 80125, Naples, Italy.
authors.forename: Virginia
authors.initials: V
authors.lastname: Brancato
authors.affiliation: Interdisciplinary Research Centre on Biomaterials, University of Naples Federico II, P.le Tecchio 80, 80125, Naples, Italy.|Department of Chemical, Materials and Industrial Production Engineering University of Naples Federico II, P.le Tecchio 80, 80125, Naples, Italy.
authors.forename: Maurizio
authors.initials: M
authors.lastname: Ventre
authors.affiliation: Center for Advanced Biomaterials for Healthcare@CRIB, Istituto Italiano di Tecnologia, L.go Barsanti e Matteucci 53, 80125, Naples, Italy.
authors.forename: Giorgia
authors.initials: G
authors.lastname: Imparato
authors.affiliation: Center for Advanced Biomaterials for Healthcare@CRIB, Istituto Italiano di Tecnologia, L.go Barsanti e Matteucci 53, 80125, Naples, Italy.
authors.forename: Francesco
authors.initials: F
authors.lastname: Urciuolo
authors.affiliation: Interdisciplinary Research Centre on Biomaterials, University of Naples Federico II, P.le Tecchio 80, 80125, Naples, Italy.
authors.forename: Concetta
authors.initials: C
authors.lastname: Meo
authors.affiliation: Interdisciplinary Research Centre on Biomaterials, University of Naples Federico II, P.le Tecchio 80, 80125, Naples, Italy.|Department of Chemical, Materials and Industrial Production Engineering University of Naples Federico II, P.le Tecchio 80, 80125, Naples, Italy.|Center for Advanced Biomaterials for Healthcare@CRIB, Istituto Italiano di Tecnologia, L.go Barsanti e Matteucci 53, 80125, Naples, Italy.
authors.forename: Paolo A
authors.initials: PA
authors.lastname: Netti
mesh_terms: D062088:Acellular Dermis / Q000378:metabolism*; D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods*; D055119:Elastic Modulus; D006801:Humans; D012756:Sheep; D054457:Tissue Scaffolds / Q000737:chemistry*; D014407:Tumor Cells, Cultured; D055114:X-Ray Microtomography
keywords: collagen networks; collagen stiffness; decellularized dermis; dehydration process; tumour cell proliferation; tumour cells