title: Dexamethasone and levetiracetam reduce hetero-cellular gap-junctional coupling between F98 glioma cells and glial cells in vitro.
abstract: Gap junctions (GJs) in astrocytes and glioma cells are important channels for cell-to-cell communication that contribute to homo- and heterocellular coupling. According to recent studies, heterocellular gap-junctional communication (H-GJC) between glioma cells and their surrounding environment enhances glioma progression. Therefore, we developed a new in vitro model to examine H-GJC between glioma cells, astrocytes and microglia. Consequently, F98 rat glioma cells were double-labeled with GJ-impermeable (CM-DiI) and GJ-permeable dye (calcein AM) and were seeded on unlabeled astrocyte-microglia co-cultures. Dual whole cell voltage clamp recordings were carried out on selected cell pairs to characterize the functional properties of H-GJC in vitro. The expression of four types of connexins (Cxs), including Cx32, Cx36, Cx43 and Cx45, and microglial phenotypes were analyzed by immunocytochemistry. The H-GJC between glioma cells and astrocytes/microglia increased after a longer incubation period with a higher number of glioma cells. We provided evidence for the direct GJ coupling of microglia and glioma cells under native in vitro conditions. In addition, we exploited this model to evaluate H-GJC after incubation with levetiracetam (LEV) and/or dexamethasone (DEX). Previous in vitro studies suggest that LEV and DEX are frequently used to control seizure and edema in glioma. Our findings showed that LEV and/or DEX decrease the number of heterocellular coupled cells significantly. In conclusion, our newly developed model demonstrated H-GJC between glioma cells and both astrocytes and microglia. The reduced H-GJC by LEV and DEX suggests a potential effect of both drugs on glioma progression.
authors.affiliation: Department of Neuroanatomy and Molecular Brain Research, Ruhr University Bochum, Universitätsstr. 150, 44801, Bochum, Germany. ismaf88@gmail.com.|Department of Neurology, University Hospital Knappschaftskrankenhaus Bochum, Ruhr University Bochum, Bochum, Germany. ismaf88@gmail.com.
authors.forename: Fatme Seval
authors.initials: FS
authors.lastname: Ismail
authors.affiliation: Department of Neuroanatomy and Molecular Brain Research, Ruhr University Bochum, Universitätsstr. 150, 44801, Bochum, Germany.|International Graduate School of Neuroscience, Ruhr University Bochum, Bochum, Germany.
authors.forename: Zahra
authors.initials: Z
authors.lastname: Moinfar
authors.affiliation: Department of Neuroanatomy and Molecular Brain Research, Ruhr University Bochum, Universitätsstr. 150, 44801, Bochum, Germany.
authors.forename: Nora
authors.initials: N
authors.lastname: Prochnow
authors.affiliation: Department of Neuroanatomy and Molecular Brain Research, Ruhr University Bochum, Universitätsstr. 150, 44801, Bochum, Germany.
authors.forename: Hannes
authors.initials: H
authors.lastname: Dambach
authors.affiliation: Department of Neuroanatomy and Molecular Brain Research, Ruhr University Bochum, Universitätsstr. 150, 44801, Bochum, Germany.
authors.forename: Daniel
authors.initials: D
authors.lastname: Hinkerohe
authors.affiliation: Department of Neurology and Clinical Neurophysiology, Evangelical Hospital Gelsenkirchen, Gelsenkirchen, Germany.
authors.forename: Claus Gert
authors.initials: CG
authors.lastname: Haase
authors.affiliation: Department of Neuroanatomy and Molecular Brain Research, Ruhr University Bochum, Universitätsstr. 150, 44801, Bochum, Germany.
authors.forename: Eckart
authors.initials: E
authors.lastname: Förster
authors.affiliation: Department of Neuroanatomy and Molecular Brain Research, Ruhr University Bochum, Universitätsstr. 150, 44801, Bochum, Germany.|International Graduate School of Neuroscience, Ruhr University Bochum, Bochum, Germany.
authors.forename: Pedro Michael
authors.initials: PM
authors.lastname: Faustmann
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology* / Q000627:therapeutic use; D001253:Astrocytes / Q000502:physiology; D002450:Cell Communication / Q000187:drug effects*; D045744:Cell Line, Tumor; D018031:Connexin 43 / Q000378:metabolism; D017630:Connexins / Q000378:metabolism; D003907:Dexamethasone / Q000494:pharmacology* / Q000627:therapeutic use; D017629:Gap Junctions / Q000187:drug effects*; D005910:Glioma / Q000188:drug therapy / Q000503:physiopathology*; D066298:In Vitro Techniques; D000077287:Levetiracetam; D017628:Microglia / Q000502:physiology; D009457:Neuroglia / Q000187:drug effects / Q000502:physiology*; D010889:Piracetam / Q000031:analogs & derivatives* / Q000494:pharmacology / Q000627:therapeutic use; D051381:Rats; D014407:Tumor Cells, Cultured; D000097002:Gap Junction beta-1 Protein; D000097004:Gap Junction delta-2 Protein
chemical_list: D000970:Antineoplastic Agents; D018031:Connexin 43; D017630:Connexins; D003907:Dexamethasone; D000077287:Levetiracetam; D010889:Piracetam; D000097002:Gap Junction beta-1 Protein; D000097004:Gap Junction delta-2 Protein; C580592:Gja1 protein, rat; C487370:Gja3 protein, rat
keywords: Astrocytes; Dexamethasone; Glioma; Heterocellular gap junctions; Levetiracetam; Microglia