title: Cerebral Microvascular and Systemic Effects Following Intravenous Administration of the Perfluorocarbon Emulsion Perftoran.
abstract: Oxygen-carrying perfluorocarbon (PFC) fluids have the potential to increase tissue oxygenation during hypoxic states and to reduce ischemic cell death. Regulatory approval of oxygen therapeutics was halted due to concerns over vasoconstrictive side effects. The goal of this study was to assess the potential vasoactive properties of Perftoran by measuring brain pial arteriolar diameters in a healthy rat model. Perftoran, crystalloid (saline) or colloid (Hextend) solutions were administered as four sequential 30 min intravenous (IV) infusions, thus allowing an evaluation of cumulative dose-dependent effects. There were no overall changes in diameters of small-sized (<50 μm) pial arterioles within the Perftoran group, while both saline and Hextend groups exhibited vasoconstriction. Medium-sized arterioles (50-100 μm) showed minor (~8-9%) vasoconstriction within saline and Hextend groups and only ~5% vasoconstriction within the Perftoran group. For small- and medium-sized pial arterioles, the mean percent change in vessel diameters was not different among the groups. Although there was a tendency for arterial blood pressures to increase with Perftoran, pressures were not different from the other two groups. These data show that Perftoran, when administered to healthy anesthetized rats, does not cause additional vasoconstriction in cerebral pial arterioles or increase systemic blood pressure compared with saline or Hextend.
authors.affiliation: NeuroTrauma Department, Naval Medical Research Center (NMRC), Silver Spring, MD 20910, USA. rania.abutarboush.ctr@mail.mil.
authors.forename: Rania
authors.initials: R
authors.lastname: Abutarboush
authors.affiliation: NeuroTrauma Department, Naval Medical Research Center (NMRC), Silver Spring, MD 20910, USA. biswajit.k.saha.ctr@mail.mil.
authors.forename: Biswajit K
authors.initials: BK
authors.lastname: Saha
authors.affiliation: NeuroTrauma Department, Naval Medical Research Center (NMRC), Silver Spring, MD 20910, USA. saad.h.mullah.ctr@mail.mil.
authors.forename: Saad H
authors.initials: SH
authors.lastname: Mullah
authors.affiliation: NeuroTrauma Department, Naval Medical Research Center (NMRC), Silver Spring, MD 20910, USA. francoise.arnaud.ctr@mail.mil.|Department of Surgery, Uniformed Services University of the Health Sciences, Bethesda, MD 20895, USA. francoise.arnaud.ctr@mail.mil.
authors.forename: Francoise G
authors.initials: FG
authors.lastname: Arnaud
authors.affiliation: NeuroTrauma Department, Naval Medical Research Center (NMRC), Silver Spring, MD 20910, USA. ashraful.haque.ctr@mail.mil.
authors.forename: Ashraful
authors.initials: A
authors.lastname: Haque
authors.affiliation: NeuroTrauma Department, Naval Medical Research Center (NMRC), Silver Spring, MD 20910, USA. chioma.aligbe@med.navy.mil.
authors.forename: Chioma
authors.initials: C
authors.lastname: Aligbe
authors.affiliation: NeuroTrauma Department, Naval Medical Research Center (NMRC), Silver Spring, MD 20910, USA. georgina.pappas@quinnipiac.edu.
authors.forename: Georgina
authors.initials: G
authors.lastname: Pappas
authors.affiliation: NeuroTrauma Department, Naval Medical Research Center (NMRC), Silver Spring, MD 20910, USA. charles.auker.ctr@mail.mil.
authors.forename: Charles R
authors.initials: CR
authors.lastname: Auker
authors.affiliation: NeuroTrauma Department, Naval Medical Research Center (NMRC), Silver Spring, MD 20910, USA. richard.m.mccarron.civ@mail.mil.|Department of Surgery, Uniformed Services University of the Health Sciences, Bethesda, MD 20895, USA. richard.m.mccarron.civ@mail.mil.
authors.forename: Richard M
authors.initials: RM
authors.lastname: McCarron
authors.affiliation: NeuroTrauma Department, Naval Medical Research Center (NMRC), Silver Spring, MD 20910, USA. pfmoonmassat@outlook.com.
authors.forename: Paula F
authors.initials: PF
authors.lastname: Moon-Massat
authors.affiliation: NeuroTrauma Department, Naval Medical Research Center (NMRC), Silver Spring, MD 20910, USA. anke.h.scultetus2.ctr@mail.mil.|Department of Surgery, Uniformed Services University of the Health Sciences, Bethesda, MD 20895, USA. anke.h.scultetus2.ctr@mail.mil.
authors.forename: Anke H
authors.initials: AH
authors.lastname: Scultetus
keywords: cerebral microcirculation; intravital microscopy; oxygen therapeutic; perfluorocarbon (PFC); vasoconstriction