title: Label free measurement of retinal blood cell flux, velocity, hematocrit and capillary width in the living mouse eye.
abstract: Measuring blood cell dynamics within the capillaries of the living eye provides crucial information regarding the health of the microvascular network. To date, the study of single blood cell movement in this network has been obscured by optical aberrations, hindered by weak optical contrast, and often required injection of exogenous fluorescent dyes to perform measurements. Here we present a new strategy to non-invasively image single blood cells in the living mouse eye without contrast agents. Eye aberrations were corrected with an adaptive optics camera coupled with a fast, 15 kHz scanned beam orthogonal to a capillary of interest. Blood cells were imaged as they flowed past a near infrared imaging beam to which the eye is relatively insensitive. Optical contrast of cells was optimized using differential scatter of blood cells in the split-detector imaging configuration. Combined, these strategies provide label-free, non-invasive imaging of blood cells in the retina as they travel in single file in capillaries, enabling determination of cell flux, morphology, class, velocity, and rheology at the single cell level.
authors.affiliation: Center for Visual Science, University of Rochester, Rochester, NY 14627, USA; The Institute of Optics, University of Rochester, Rochester, NY 14620, USA.
authors.forename: A
authors.initials: A
authors.lastname: Guevara-Torres
authors.affiliation: Center for Visual Science, University of Rochester, Rochester, NY 14627, USA; The Institute of Optics, University of Rochester, Rochester, NY 14620, USA.
authors.forename: A
authors.initials: A
authors.lastname: Joseph
authors.affiliation: Center for Visual Science, University of Rochester, Rochester, NY 14627, USA; Flaum Eye Institute, University of Rochester, Rochester, NY 14642, USA; Department of Neuroscience, University of Rochester, Rochester, NY 14642, USA.
authors.forename: J B
authors.initials: JB
authors.lastname: Schallek
keywords: (110.1080) Active or adaptive optics; (170.4460) Ophthalmic optics and devices; (330.4300) Vision system - noninvasive assessment; (330.7324) Visual optics, comparative animal models