title: Characterization of Iranian nonaflatoxigenic strains ofAspergillus flavusbased on microsatellite-primed PCR.
abstract: Out of fifty-two Iranian nonaflatoxigenic strains ofAspergillus flavus,collected from various substrates (soil and kernel) and sources (peanut, corn and pistachio),fifteen representatives were selected according to their different geographical origins (six provinces: Guilan and Golestan, Ardebil, Fars, Kerman and Semnan) and vegetative compatibility groups (VCGs, IR1 to IR15) for microsatellite-primed PCR analysis. Two inter-simple sequence repeat (ISSR) primers AFMPP and AFM13 were used to determine polymorphism and the relationship among strain isolates.A. flavusisolates were identified by their morphologies and their identities were confirmed by PCR amplification using the specific primer pair ITS1 and ITS4. The results revealed variations in the percentages of polymorphisms. In the ISSR analysis, primers AFMPP and AFM13 generated a total of 18 and 23 amplicons among the fungal strains, out of which 12 (66.7%) and 22 (95.7%) were polymorphic, respectively. Cluster analysis of the ISSR data was carried out using 1 D DNA gel image analysis. The two dendrograms obtained through these markers showed six different clusterings of testing nonaflatoxigenicA. flavusL strains, but we noticed that some clusters were different in some cases. The microsatellite-primed PCR data revealed that the Iranian nonaflatoxigenic isolates ofA. flavuswere not clustered according to their origins and sources. This study is the first to characterize Iranian nonaflatoxigenic isolates ofA. flavususing ISSR markers.
authors.affiliation: Department of Plant Protection, Faculty of Agriculture, Ferdowsi University of Mashhad, Iran.
authors.forename: Mahmoud
authors.initials: M
authors.lastname: Houshyarfard
authors.affiliation: Department of Plant Protection, Faculty of Agriculture, Ferdowsi University of Mashhad, Iran.
authors.forename: Hamid
authors.initials: H
authors.lastname: Rouhani
authors.affiliation: Department of Plant Protection, Faculty of Agriculture, Ferdowsi University of Mashhad, Iran.
authors.forename: Mahrokh
authors.initials: M
authors.lastname: Falahati-Rastegar
authors.affiliation: Department of Crop Biotechnology and Breeding, Faculty of Agriculture, Ferdowsi University of Mashhad, Iran.
authors.forename: Saeid
authors.initials: S
authors.lastname: Malekzadeh-Shafaroudi
authors.affiliation: Department of Plant Protection, Faculty of Agriculture, Ferdowsi University of Mashhad, Iran.
authors.forename: Esmat
authors.initials: E
authors.lastname: Mahdikhani-Moghaddam
keywords: Aflatoxin; Inter-simple sequence repeat; Molecular marker; Polymorphism