title: Lipid Rafts Promote trans Fatty Acid-Induced Inflammation in Human Umbilical Vein Endothelial Cells.
abstract: The effects of two fatty acids, oleic acid (OLA) and elaidic acid (ELA) on normal human umbilical vein endothelial cells (HUVEC) and non-rafts HUVEC were investigated in this study. The expression levels of inflammatory cytokines (ICAM-1, VCAM-1 and IL-6) were analyzed. Western blot was used to analyze the expression levels of inflammation-related proteins (NF-κB, ERK1/2) and toll-like receptors 4 (TLR4). The results showed that the levels of nuclear translocation of NF-κB p65 and phosphorylated ERK1/2 were significantly decreased only in non-lipid rafts cells pretreated with trans fatty acid (TFA). The expression of TLR4 in the ELA-treated normal cells was higher than that in non-lipid rafts HUVEC. When the lipid rafts was destroyed by methyl-β-cyclodextrin, the levels of nuclear translocation of NF-κB p65, phosphorylated ERK1/2 and TLR4 were decreased significantly. Therefore, lipid rafts may be involved in TFA induced-inflammation in HUVEC through blocking the inflammatory signal pathway. Lipid rafts might be a platform for specific receptors such as TLR4 for TFA to activate the pro-inflammation on cell membranes.
authors.affiliation: State Key Laboratory of Food Science and Technology, University of Nanchang, Nanchang, 330047, Jiangxi, China.
authors.forename: Yao
authors.initials: Y
authors.lastname: Pan
authors.affiliation: State Key Laboratory of Food Science and Technology, University of Nanchang, Nanchang, 330047, Jiangxi, China.
authors.forename: Benxin
authors.initials: B
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Food Science and Technology, University of Nanchang, Nanchang, 330047, Jiangxi, China.|Institute for Advanced Study, University of Nanchang, Nanchang, 330031, Jiangxi, China.
authors.forename: Zeyuan
authors.initials: Z
authors.lastname: Deng
authors.affiliation: State Key Laboratory of Food Science and Technology, University of Nanchang, Nanchang, 330047, Jiangxi, China.
authors.forename: Yawei
authors.initials: Y
authors.lastname: Fan
authors.affiliation: State Key Laboratory of Food Science and Technology, University of Nanchang, Nanchang, 330047, Jiangxi, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Li
authors.affiliation: State Key Laboratory of Food Science and Technology, University of Nanchang, Nanchang, 330047, Jiangxi, China. lihongyan@ncu.edu.cn.
authors.forename: Hongyan
authors.initials: H
authors.lastname: Li
mesh_terms: D002467:Cell Nucleus / Q000276:immunology; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D007249:Inflammation / Q000378:metabolism*; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism; D015850:Interleukin-6; D020935:MAP Kinase Signaling System / Q000187:drug effects; D021962:Membrane Microdomains / Q000276:immunology*; D016328:NF-kappa B / Q000378:metabolism; D019301:Oleic Acid / Q000494:pharmacology*; D009829:Oleic Acids; D010766:Phosphorylation / Q000187:drug effects; D021381:Protein Transport / Q000187:drug effects; D051197:Toll-Like Receptor 4; D044242:Trans Fatty Acids / Q000494:pharmacology*; D019010:Vascular Cell Adhesion Molecule-1 / Q000378:metabolism
chemical_list: C573420:ICAM1 protein, human; C508600:IL6 protein, human; D015850:Interleukin-6; D016328:NF-kappa B; D009829:Oleic Acids; C495345:TLR4 protein, human; D051197:Toll-Like Receptor 4; D044242:Trans Fatty Acids; D019010:Vascular Cell Adhesion Molecule-1; D018799:Intercellular Adhesion Molecule-1; D019301:Oleic Acid; C011459:elaidic acid
keywords: ERK1/2; Inflammation; Lipid rafts; NF-kB; trans Fatty acids