title: GPR39 is region-specifically expressed in mouse oviduct correlating with the Zn2+distribution.
abstract: G-protein-coupled receptor 39 (GPR39) plays a role in cellular and physiological processes, including insulin secretion, cell death inhibition, wound healing, and obesity. Increasing evidence suggests that GPR39 is potently stimulated by zinc ions (Zn2+) and is therefore considered a putative Zn2+receptor. Given the importance of Zn2+in the reproductive system, we proposed that GPR39 might have a functional role in the reproductive system. However, the localization of GPR39 in the reproductive system remains unknown. Here, we used mice expressing a Gpr39 promoter-driven LacZ reporter system to detect Gpr39 expression in the reproductive system at different phases of the estrous cycle and found an interesting region-specific distribution of Gpr39 in the mouse oviduct epithelium, with strong expression at the ampulla and weak expression at the isthmus, which was consistent with the results using reverse transcription polymerase chain reaction and immunofluorescence. Moreover, using ZnSeAMGstaining, we found that Zn2+, the putative ligand of GPR39, also found a distribution similar to GPR39 expression, suggesting that their potential interaction mediates fertilization and embryo transportation.
authors.affiliation: College of Animal Science and Technology, Beijing University of Agriculture, Beijing, China.
authors.forename: Jingqiao
authors.initials: J
authors.lastname: Qiao
authors.affiliation: State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China.
authors.forename: Huashan
authors.initials: H
authors.lastname: Zhao
authors.affiliation: State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China.
authors.forename: Hongying
authors.initials: H
authors.lastname: Peng
authors.affiliation: State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Chen
authors.affiliation: College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, China.
authors.forename: He
authors.initials: H
authors.lastname: Zhang
authors.affiliation: College of Animal Science and Technology, Beijing University of Agriculture, Beijing, China.
authors.forename: Xueying
authors.initials: X
authors.lastname: Zheng
authors.affiliation: College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, China.
authors.forename: Yaping
authors.initials: Y
authors.lastname: Jin
authors.affiliation: College of Animal Science and Technology, Beijing University of Agriculture, Beijing, China.
authors.forename: Hemin
authors.initials: H
authors.lastname: Ni
authors.affiliation: State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China. Electronic address: duane@ioz.ac.cn.
authors.forename: Enkui
authors.initials: E
authors.lastname: Duan
authors.affiliation: College of Animal Science and Technology, Beijing University of Agriculture, Beijing, China. Electronic address: y63guo@126.com.
authors.forename: Yong
authors.initials: Y
authors.lastname: Guo
mesh_terms: D000818:Animals; D030762:Estrous Cycle; D005187:Fallopian Tubes / Q000378:metabolism*; D005260:Female; D005786:Gene Expression Regulation / Q000502:physiology*; D051379:Mice; D043562:Receptors, G-Protein-Coupled / Q000235:genetics / Q000378:metabolism*; D015032:Zinc / Q000378:metabolism*
chemical_list: C512928:GPR39 protein, mouse; D043562:Receptors, G-Protein-Coupled; D015032:Zinc
keywords: Estrous cycle; GPR39; Mice; Oviduct; Zinc