title: Lysosomal membrane protein SIDT2 mediates the direct uptake of DNA by lysosomes.
abstract: Lysosomes degrade macromolecules such as proteins and nucleic acids. We previously identified 2 novel types of autophagy, RNautophagy and DNautophagy, where lysosomes directly take up RNA and DNA, in an ATP-dependent manner, for degradation. We have also reported that SIDT2 (SID1 transmembrane family, member 2), an ortholog of the Caenorhabditis elegans putative RNA transporter SID-1 (systemic RNA interference defective-1), mediates RNA translocation during RNautophagy. In this addendum, we report that SIDT2 also mediates DNA translocation in the process of DNautophagy. These findings help elucidate the mechanisms underlying the direct uptake of nucleic acids by lysosomes and the physiological functions of DNautophagy.
authors.affiliation: a Department of Degenerative Neurological Diseases , National Institute of Neuroscience, National Center of Neurology and Psychiatry , Kodaira, Tokyo , Japan.
authors.forename: Shu
authors.initials: S
authors.lastname: Aizawa
authors.affiliation: a Department of Degenerative Neurological Diseases , National Institute of Neuroscience, National Center of Neurology and Psychiatry , Kodaira, Tokyo , Japan.|b Department of Neurology , Interdisciplinary Graduate School of Medicine and Engineering, University of Yamanashi , Chuo, Yamanashi , Japan.
authors.forename: Viorica Raluca
authors.initials: VR
authors.lastname: Contu
authors.affiliation: a Department of Degenerative Neurological Diseases , National Institute of Neuroscience, National Center of Neurology and Psychiatry , Kodaira, Tokyo , Japan.
authors.forename: Yuuki
authors.initials: Y
authors.lastname: Fujiwara
authors.affiliation: a Department of Degenerative Neurological Diseases , National Institute of Neuroscience, National Center of Neurology and Psychiatry , Kodaira, Tokyo , Japan.
authors.forename: Katsunori
authors.initials: K
authors.lastname: Hase
authors.affiliation: a Department of Degenerative Neurological Diseases , National Institute of Neuroscience, National Center of Neurology and Psychiatry , Kodaira, Tokyo , Japan.
authors.forename: Hisae
authors.initials: H
authors.lastname: Kikuchi
authors.affiliation: a Department of Degenerative Neurological Diseases , National Institute of Neuroscience, National Center of Neurology and Psychiatry , Kodaira, Tokyo , Japan.
authors.forename: Chihana
authors.initials: C
authors.lastname: Kabuta
authors.affiliation: a Department of Degenerative Neurological Diseases , National Institute of Neuroscience, National Center of Neurology and Psychiatry , Kodaira, Tokyo , Japan.
authors.forename: Keiji
authors.initials: K
authors.lastname: Wada
authors.affiliation: a Department of Degenerative Neurological Diseases , National Institute of Neuroscience, National Center of Neurology and Psychiatry , Kodaira, Tokyo , Japan.
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Kabuta
mesh_terms: D000818:Animals; D001343:Autophagy; D045744:Cell Line, Tumor; D002462:Cell Membrane / Q000378:metabolism*; D004247:DNA / Q000378:metabolism*; D049452:Green Fluorescent Proteins / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D008247:Lysosomes / Q000378:metabolism*; D008565:Membrane Proteins / Q000378:metabolism*; D051379:Mice; D033704:Nucleotide Transport Proteins / Q000378:metabolism*; D012313:RNA / Q000378:metabolism; D034741:RNA, Small Interfering / Q000378:metabolism
chemical_list: D008565:Membrane Proteins; D033704:Nucleotide Transport Proteins; D034741:RNA, Small Interfering; C000613088:SIDT2 protein, human; C554947:Sidt2 protein, mouse; D049452:Green Fluorescent Proteins; D012313:RNA; D004247:DNA
keywords: DNA; DNautophagy; RNA; RNautophagy; autophagy; lysosome; membrane protein