title: H2S biosynthesis and catabolism: new insights from molecular studies.
abstract: Hydrogen sulfide (H2S) has profound biological effects within living organisms and is now increasingly being considered alongside other gaseous signalling molecules, such as nitric oxide (NO) and carbon monoxide (CO). Conventional use of pharmacological and molecular approaches has spawned a rapidly growing research field that has identified H2S as playing a functional role in cell-signalling and post-translational modifications. Recently, a number of laboratories have reported the use of siRNA methodologies and genetic mouse models to mimic the loss of function of genes involved in the biosynthesis and degradation of H2S within tissues. Studies utilising these systems are revealing new insights into the biology of H2S within the cardiovascular system, inflammatory disease, and in cell signalling. In light of this work, the current review will describe recent advances in H2S research made possible by the use of molecular approaches and genetic mouse models with perturbed capacities to generate or detoxify physiological levels of H2S gas within tissues.
authors.affiliation: School of Life Science, University of Lincoln, Brayford Pool, Lincoln, Lincolnshire, LN6 7TS, UK. prose@lincoln.ac.uk.|State Key Laboratory of Quality Research in Chinese Medicine and School of Pharmacy, Macau University of Science and Technology, Macau, China. prose@lincoln.ac.uk.
authors.forename: Peter
authors.initials: P
authors.lastname: Rose
authors.affiliation: Department of Pharmacology, National University of Singapore, Lee Kong Chian Wing, UHL #05-02R, 21 Lower Kent Ridge Road, Singapore, 119077, Singapore.
authors.forename: Philip K
authors.initials: PK
authors.lastname: Moore
authors.affiliation: State Key Laboratory of Quality Research in Chinese Medicine and School of Pharmacy, Macau University of Science and Technology, Macau, China.
authors.forename: Yi Zhun
authors.initials: YZ
authors.lastname: Zhu
mesh_terms: D000818:Animals; D053898:Biosynthetic Pathways; D019757:Carbon-Oxygen Lyases / Q000235:genetics / Q000378:metabolism; D003541:Cystathionine beta-Synthase / Q000235:genetics / Q000378:metabolism; D050558:Cysteine Dioxygenase / Q000235:genetics / Q000378:metabolism; D049308:Dioxygenases / Q000235:genetics / Q000378:metabolism; D005786:Gene Expression Regulation; D055786:Gene Knockout Techniques / Q000379:methods; D006801:Humans; D006862:Hydrogen Sulfide / Q000378:metabolism*; D024101:Mitochondrial Proteins / Q000235:genetics / Q000378:metabolism; D011808:Quinone Reductases / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction; D013466:Sulfurtransferases / Q000235:genetics / Q000378:metabolism
chemical_list: D024101:Mitochondrial Proteins; D049308:Dioxygenases; C538757:ETHE1 protein, mouse; D050558:Cysteine Dioxygenase; D011808:Quinone Reductases; C073412:sulfide quinone reductase; C022696:O-succinylhomoserine (thiol)-lyase; D013466:Sulfurtransferases; C021550:3-mercaptopyruvate sulphurtransferase; D019757:Carbon-Oxygen Lyases; D003541:Cystathionine beta-Synthase; D006862:Hydrogen Sulfide
keywords: Biosynthesis; Catabolism; Hydrogen sulfide; Molecular models