title: Diversity of bat astroviruses in Lao PDR and Cambodia.
abstract: Astroviruses are known to infect humans and a wide range of animal species, and can cause gastroenteritis in their hosts. Recent studies have reported astroviruses in bats in Europe and in several locations in China. We sampled 1876 bats from 17 genera at 45 sites from 14 and 13 provinces in Cambodia and Lao PDR respectively, and tested them for astroviruses. Our study revealed a high diversity of astroviruses among various Yangochiroptera and Yinpterochiroptera bats. Evidence for varying degrees of host restriction for astroviruses in bats was found. Furthermore, additional Pteropodid hosts were detected. The astroviruses formed distinct phylogenetic clusters within the genus Mamastrovirus, most closely related to other known bat astroviruses. The astrovirus sequences were found to be highly saturated indicating that phylogenetic relationships should be interpreted carefully. An astrovirus clustering in a group with other viruses from diverse hosts, including from ungulates and porcupines, was found in a Rousettus bat. These findings suggest that diverse astroviruses can be found in many species of mammals, including bats.
authors.affiliation: Institut Pasteur du Cambodge, Virology Unit, Phnom Penh, Cambodia.
authors.forename: Audrey
authors.initials: A
authors.lastname: Lacroix
authors.affiliation: Institut Pasteur du Cambodge, Virology Unit, Phnom Penh, Cambodia.
authors.forename: Veasna
authors.initials: V
authors.lastname: Duong
authors.affiliation: Institut Pasteur du Cambodge, Virology Unit, Phnom Penh, Cambodia.
authors.forename: Vibol
authors.initials: V
authors.lastname: Hul
authors.affiliation: National Veterinary Research Institute, Department of Animal Health and Production, Ministry of Agriculture Forestry and Fisheries, Cambodia.
authors.forename: Sorn
authors.initials: S
authors.lastname: San
authors.affiliation: National Veterinary Research Institute, Department of Animal Health and Production, Ministry of Agriculture Forestry and Fisheries, Cambodia.
authors.forename: Holl
authors.initials: H
authors.lastname: Davun
authors.affiliation: Forest Administration, Ministry of Agriculture Forestry and Fisheries, Cambodia.
authors.forename: Keo
authors.initials: K
authors.lastname: Omaliss
authors.affiliation: Wildlife Conservation Society, Cambodia.
authors.forename: Sokha
authors.initials: S
authors.lastname: Chea
authors.affiliation: Institut de Systématique, Evolution, Biodiversité (ISYEB), UMR 7205 MNHN CNRS UPMC, EPHE, Muséum national d'Histoire naturelle, Paris, France; Muséum national d'Histoire naturelle, Institut de Systématique, Evolution, Biodiversité (ISYEB), UMR 7205 MNHN CNRS UPMC, France.
authors.forename: Alexandre
authors.initials: A
authors.lastname: Hassanin
authors.affiliation: National Animal Health Laboratory, Ministry of Agriculture Forestry and Fisheries, Laos.
authors.forename: Watthana
authors.initials: W
authors.lastname: Theppangna
authors.affiliation: Wildlife Conservation Society, Laos; Metabiota Inc., Vientiane, Laos.
authors.forename: Soubanh
authors.initials: S
authors.lastname: Silithammavong
authors.affiliation: Wildlife Conservation Society, Laos.
authors.forename: Kongsy
authors.initials: K
authors.lastname: Khammavong
authors.affiliation: Wildlife Conservation Society, Laos.
authors.forename: Sinpakone
authors.initials: S
authors.lastname: Singhalath
authors.affiliation: Institute of Physical Geography, Faculty of Geography and Regional Studies, University of Warsaw, Warsaw, Poland.
authors.forename: Aneta
authors.initials: A
authors.lastname: Afelt
authors.affiliation: Wildlife Conservation Society, Laos.
authors.forename: Zoe
authors.initials: Z
authors.lastname: Greatorex
authors.affiliation: Wildlife Conservation Society, Wildlife Health Program, Bronx, N.Y., USA.
authors.forename: Amanda E
authors.initials: AE
authors.lastname: Fine
authors.affiliation: One Health Institute, School of Veterinary Medicine, University of California, Davis, USA.
authors.forename: Tracey
authors.initials: T
authors.lastname: Goldstein
authors.affiliation: Wildlife Conservation Society, Wildlife Health Program, Bronx, N.Y., USA.
authors.forename: Sarah
authors.initials: S
authors.lastname: Olson
authors.affiliation: Wildlife Conservation Society, Wildlife Health Program, Bronx, N.Y., USA; Metabiota Inc., Nanaimo, British Columbia, Canada.
authors.forename: Damien O
authors.initials: DO
authors.lastname: Joly
authors.affiliation: Wildlife Conservation Society, Cambodia.
authors.forename: Lucy
authors.initials: L
authors.lastname: Keatts
authors.affiliation: Institut Pasteur du Cambodge, Virology Unit, Phnom Penh, Cambodia.
authors.forename: Philippe
authors.initials: P
authors.lastname: Dussart
authors.affiliation: Cirad, UMR 17, Cirad-Ird, TA-A17/G, Montpellier, France; Université de Montpellier, IES, UMR 5214, CNRS-UM, Montpellier, France. Electronic address: roger.frutos@ies.univ-montp2.fr.
authors.forename: Roger
authors.initials: R
authors.lastname: Frutos
authors.affiliation: Institut Pasteur du Cambodge, Virology Unit, Phnom Penh, Cambodia; GSK Vaccines R&D, 150 Beach road, # 22-00, 189720, Singapore. Electronic address: buchyphilippe@hotmail.com.
authors.forename: Philippe
authors.initials: P
authors.lastname: Buchy
mesh_terms: D000818:Animals; D019200:Astroviridae / Q000145:classification / Q000235:genetics*; D019350:Astroviridae Infections / Q000662:veterinary* / Q000821:virology*; D002160:Cambodia; D002685:Chiroptera / Q000821:virology*; D005243:Feces / Q000821:virology; D014644:Genetic Variation / Q000235:genetics*; D007812:Laos; D008168:Lung / Q000821:virology; D009055:Mouth / Q000821:virology; D010802:Phylogeny; D012007:Rectum / Q000821:virology
keywords: Astroviruses; Bats; Cambodia; Genetic diversity; Lao PDR