title: Ameliorating effects of green tea extract on cadmium induced reproductive injury in male Wistar rats with respect to androgen receptors and caspase- 3.
abstract: Cadmium is one of the potent environmental endocrine disruptors that has adverse effects on male fertility. This study was conducted to investigate the protective role of green tea extract (GTE) on cadmium chloride (CdCl2) induced reproductive toxicities in male Wistar rats. Thirty-six male Wistar rats were divided to 4 groups, each one contained 9 rats. Control group, they were gavaged distilled water, meanwhile the treated groups gavaged CdCl2(3mg/kg), GTE (70mg/kg) and CdCl2+GTE (3mg/kg and 70mg/kg) for 63days. In GTE+ CdCl2treated rats, the final body weight, relative testicular weight, epididymal weight, seminal and prostate glands weights were significantly (P<0.05) increased compared to CdCl2intoxicated rats. Sperm cell concentration was significantly (P<0.05) increased in CdCl2+GTE group, while sperm morphological abnormalities were significantly (P<0.05) decreased than CdCl2group. The levels of superoxide dismutase (SOD) and reduced glutathione (GSH) were elevated significantly (P<0.05) in CdCl2+GTE group, meanwhile catalase activity was significantly (P<0.05) declined than CdCl2group. Hypercholesterolemia was observed in CdCl2group than control one while GTE+CdCl2group revealed significant (P<0.05) reduction in cholesterol level than CdCl2treated group. Testicular androgen receptors and caspase-3 protein expression showed marked reduction and increase (P<0.05) in CdCl2group than control, respectively. Treatment with GTE with CdCl2significantly (P<0.05) increased androgen receptors, while decreased caspase-3 protein than CdCl2group. In conclusion, GTE exhibited protective effect on testes by inhibiting CdCl2induced oxidative damage and cellular apoptosis.
authors.affiliation: Department of Physiology, Faculty of Veterinary Medicine, Suez Canal University, Ismailia, 41522, Egypt. Electronic address: hebaabdelrazekvet@gmail.com.
authors.forename: Heba M A
authors.initials: HM
authors.lastname: Abdelrazek
authors.affiliation: Department of Cytology and Histology, Faculty of Veterinary Medicine, Suez Canal University, Ismailia, Egypt; Female collage of Health Sciences, University of Bisha, Saudi Arabia.
authors.forename: Seham A
authors.initials: SA
authors.lastname: Helmy
authors.affiliation: Department Of Theriogenology, Faculty of Veterinary Medicine, Suez Canal University, Ismailia, Egypt.
authors.forename: Doaa H
authors.initials: DH
authors.lastname: Elsayed
authors.affiliation: Department of Zoology, Faculty of Sciences, Suez Canal University, Ismailia, Egypt.
authors.forename: Hala M
authors.initials: HM
authors.lastname: Ebaid
authors.affiliation: Department of Forensic medicine & Clinical Toxicology, Faculty of Medicine, Suez Canal University, Ismailia, Egypt.
authors.forename: Racha M
authors.initials: RM
authors.lastname: Mohamed
mesh_terms: D000818:Animals; D019256:Cadmium Chloride / Q000633:toxicity*; D053148:Caspase 3 / Q000378:metabolism*; D004822:Epididymis / Q000187:drug effects / Q000378:metabolism; D008297:Male; D009929:Organ Size / Q000187:drug effects; D018384:Oxidative Stress / Q000187:drug effects*; D010936:Plant Extracts / Q000494:pharmacology*; D011467:Prostate / Q000187:drug effects / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D011944:Receptors, Androgen / Q000378:metabolism*; D013094:Spermatozoa / Q000187:drug effects / Q000378:metabolism; D013662:Tea; D013737:Testis / Q000187:drug effects* / Q000378:metabolism
chemical_list: D010936:Plant Extracts; D011944:Receptors, Androgen; D013662:Tea; D053148:Caspase 3; D019256:Cadmium Chloride
keywords: Androgen receptors; Cadmium; Caspase-3; Green tea; Testis