title: A simple and reliable analytical method based on HPLC-UV to determine oleanonic acid content in Chios gum mastic for quality control.
abstract: A reliable analytical method based on high-performance liquid chromatography-ultraviolet detection was established for the determination of oleanonic acid (OA) content in Chios gum mastic (CGM). A simple method involving methanol extraction of CGM powder followed by basification and ether extraction was developed to isolate the triterpenic fraction including OA. The triterpenic fraction was chromatographed on a Phenomenex Gemini C18column (150 × 4.6 mm, 5 µm) under a simple gradient elution of a mobile phase containing acetonitrile and water at a flow rate of 1.0 mL min-1. The detection wavelength was set at 210 nm. Good linearity was achieved in the range of 100.0-1000.0 μg mL-1with r2> 0.9993, and the limit of quantification was 32.22 μg mL-1. Accuracy measured at three concentration levels was in the range of 93.72-99.56%, while intra-day and inter-day precisions estimated using both OA standard and CGM samples were no more than 2.83 and 4.57% RSD, respectively. Finally, this method was applied to real CGM samples from various batches, revealing that the OA contents were between 88.13 and 100.83 μg mg-1. These results suggest that the current method can be applied as an efficient analytical method for quality control of CGM.
authors.affiliation: School of Pharmacy, Sungkyunkwan University, Suwon, Gyeonggi, 16419, Republic of Korea.
authors.forename: Yan
authors.initials: Y
authors.lastname: Jin
authors.affiliation: School of Pharmacy, Sungkyunkwan University, Suwon, Gyeonggi, 16419, Republic of Korea.
authors.forename: Jing
authors.initials: J
authors.lastname: Zhao
authors.affiliation: School of Pharmacy, Sungkyunkwan University, Suwon, Gyeonggi, 16419, Republic of Korea.
authors.forename: Kyung Min
authors.initials: KM
authors.lastname: Jeong
authors.affiliation: School of Pharmacy, Sungkyunkwan University, Suwon, Gyeonggi, 16419, Republic of Korea.
authors.forename: Da Eun
authors.initials: DE
authors.lastname: Yoo
authors.affiliation: School of Pharmacy, Sungkyunkwan University, Suwon, Gyeonggi, 16419, Republic of Korea.
authors.forename: Se Young
authors.initials: SY
authors.lastname: Han
authors.affiliation: Nutribiotech Co., Ltd., Seoul, 06248, Republic of Korea.
authors.forename: Su-Young
authors.initials: SY
authors.lastname: Choi
authors.affiliation: Nutribiotech Co., Ltd., Seoul, 06248, Republic of Korea.
authors.forename: Dong-Hyeon
authors.initials: DH
authors.lastname: Ko
authors.affiliation: Nutribiotech Co., Ltd., Seoul, 06248, Republic of Korea.
authors.forename: Hyun-Ji
authors.initials: HJ
authors.lastname: Kim
authors.affiliation: Nutribiotech Co., Ltd., Seoul, 06248, Republic of Korea.
authors.forename: Na-Hye
authors.initials: NH
authors.lastname: Sung
authors.affiliation: School of Pharmacy, Sungkyunkwan University, Suwon, Gyeonggi, 16419, Republic of Korea. jlee0610@skku.edu.
authors.forename: Jeongmi
authors.identifier: http://orcid.org/0000-0002-9948-7554
authors.initials: J
authors.lastname: Lee
mesh_terms: D002626:Chemistry, Pharmaceutical / Q000379:methods* / Q000592:standards*; D002851:Chromatography, High Pressure Liquid / Q000379:methods / Q000592:standards; D000069997:Mastic Resin / Q000032:analysis* / Q000737:chemistry; D011786:Quality Control; D015203:Reproducibility of Results; D014315:Triterpenes / Q000032:analysis* / Q000737:chemistry
chemical_list: D000069997:Mastic Resin; D014315:Triterpenes; C450739:oleanonic acid
keywords: Chios gum mastic; Method validation; Oleanonic acid; Quality control