title: Formulation and comparative in vitro evaluation of various dexamethasone-loaded pH-sensitive polymeric nanoparticles intended for dermal applications.
abstract: pH-sensitive nanoparticles have a great potential for dermal and transfollicular drug delivery. In this study, pH-sensitive, dexamethasone-loaded Eudragit®L 100, Eudragit®L 100-55, Eudragit®S 100, HPMCP-50, HPMCP-55 and cellulose acetate phthalate nanoparticles were prepared by nanoprecipitation and characterized. The pH-dependent swelling, erosion, dissolution and drug release kinetics were investigated in vitro using dynamic light scattering and Franz diffusion cells, respectively. Their toxicity potential was assessed by the ROS and MTT assays. 100-700nm nanoparticles with high drug loading and entrapment efficiency were obtained. The nanoparticles bear no toxicity potential. Cellulose phthalates nanoparticles were more sensitive to pH than acrylates nanoparticles. They dissolved in 10mM pH 7.5 buffer and released>80% of the drug within 7h. The acrylate nanoparticles dissolved in 40mM pH 7.5 buffer and released 65-70% of the drug within 7h. The nanoparticles remained intact in 10 and 40mM pH 6.0 buffers (HPMCP nanoparticles dissolved in 40mM pH 6.0 buffer) and released slowly. The nanoparticles properties could be modulated by blending the different polymers. In conclusion, various pH-sensitive nanoparticles that could release differently on the skin surface and dissolve and release in the hair follicles were obtained.
authors.affiliation: College of Pharmacy, Freie Universität Berlin, Kelchstr. 31, D-12169, Berlin, Germany.
authors.forename: Fitsum Feleke
authors.initials: FF
authors.lastname: Sahle
authors.affiliation: Institute of Nutritional Science, University of Potsdam, Arthur-Scheunert-Allee 114-116, D-14558 Nuthetal, Potsdam, Germany.
authors.forename: Christian
authors.initials: C
authors.lastname: Gerecke
authors.affiliation: Institute of Nutritional Science, University of Potsdam, Arthur-Scheunert-Allee 114-116, D-14558 Nuthetal, Potsdam, Germany.
authors.forename: Burkhard
authors.initials: B
authors.lastname: Kleuser
authors.affiliation: College of Pharmacy, Freie Universität Berlin, Kelchstr. 31, D-12169, Berlin, Germany. Electronic address: bodmeier@zedat.fu-berlin.de.
authors.forename: Roland
authors.initials: R
authors.lastname: Bodmeier
mesh_terms: D000279:Administration, Cutaneous; D002626:Chemistry, Pharmaceutical / Q000379:methods; D003692:Delayed-Action Preparations; D003907:Dexamethasone / Q000008:administration & dosage* / Q000737:chemistry / Q000493:pharmacokinetics; D004337:Drug Carriers / Q000737:chemistry*; D016503:Drug Delivery Systems; D065546:Drug Liberation; D005938:Glucocorticoids / Q000737:chemistry / Q000493:pharmacokinetics; D006863:Hydrogen-Ion Concentration; D053758:Nanoparticles; D010316:Particle Size; D011108:Polymers / Q000737:chemistry*; D012995:Solubility; D013997:Time Factors
chemical_list: D003692:Delayed-Action Preparations; D004337:Drug Carriers; D005938:Glucocorticoids; D011108:Polymers; D003907:Dexamethasone
keywords: Acetic acid (PubChem CID: 176); Cellulose acetate phthalate; Cellulose acetate phthalate (PubChem CID: 44135440); Dexamethasone; Dexamethasone (PubChem CID: 5743); Eudragit(®); Eudragits (PubChem CID: 65358); H2DCFDA (PubChem CID: 77718); HPMCP; HPMCP (PubChem CID: 57469); MTT (PubChem CID: 64965); Polyvinyl alcohol (PubChem CID: 11199); Potassium dihydrogen phosphate (PubChem CID: 516951); Skin nanocarrier; pH-sensitive nanoparticle