title: miR-27b inhibits gastric cancer metastasis by targeting NR2F2.
abstract: Increasing attention is focused on the down-regulation of miRNAs in cancer process. Nuclear receptor subfamily 2 (NR2F2, also known as COUP-TFII) is involved in the development of many types of cancers, but its role in gastric cancer remains elusive. In this experiment, oncomine and Kaplan-meier database revealed that NR2F2 was up-regulated in gastric cancer and that the high NR2F2 expression contributed to poor survival. MicroRNA-27b was targeted and down-regulated by NR2F2 in human gastric cancer tissues and cells. The ectopic expression of miR-27b inhibited gastric cancer cell proliferation and tumor growth in vitro and in vivo. Assays suggested that the overexpression of miR-27b could promote MGC-803 cells' migration and invasion and retard their metastasis to the liver. In addition, down-regulation of miR-27b enhanced GES-1 cells' proliferation and metastasis in vitro. These findings reveal that miR-27b is a tumor suppressor in gastric cancer and a biomarker for improving patients' survival.
authors.affiliation: Department of General Surgery, The Affiliated Drum Tower Hospital of Medical School of Nanjing University, Nanjing, 210008, China. izaobao@vip.qq.com.
authors.forename: Qingzhao
authors.initials: Q
authors.lastname: Feng
authors.affiliation: Department of Emergency, The Yiwu Affiliated Hospital of Wenzhou Medical University, Yiwu, 322000, China.
authors.forename: Xionglin
authors.initials: X
authors.lastname: Wu
authors.affiliation: Department of Gerontology, The Affiliated Drum Tower Hospital of Medical School of Nanjing University, Nanjing, 210008, China.
authors.forename: Fuchao
authors.initials: F
authors.lastname: Li
authors.affiliation: The Children's Hospital of Zhejiang University School of Medicine, Hangzhou, 310003, China.
authors.forename: Beibei
authors.initials: B
authors.lastname: Ning
authors.affiliation: Department of General Surgery, The Affiliated Drum Tower Hospital of Medical School of Nanjing University, Nanjing, 210008, China.
authors.forename: Xiaofeng
authors.initials: X
authors.lastname: Lu
authors.affiliation: Department of General Surgery, The Affiliated Drum Tower Hospital of Medical School of Nanjing University, Nanjing, 210008, China.
authors.forename: Yin
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Orthopaedics, The Affiliated Drum Tower Hospital of Medical School of Nanjing University, Nanjing, 210008, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Pan
authors.affiliation: Department of General Surgery, The Affiliated Drum Tower Hospital of Medical School of Nanjing University, Nanjing, 210008, China. guan-wx@163.com.
authors.forename: Wenxian
authors.initials: W
authors.lastname: Guan
mesh_terms: D000818:Animals; D014408:Biomarkers, Tumor / Q000235:genetics / Q000378:metabolism*; D051837:COUP Transcription Factor II / Q000235:genetics / Q000378:metabolism*; D045744:Cell Line, Tumor; D005260:Female; D016147:Genes, Tumor Suppressor; D064593:Heterografts; D006801:Humans; D008297:Male; D008819:Mice, Nude; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D009362:Neoplasm Metastasis; D009363:Neoplasm Proteins / Q000235:genetics / Q000378:metabolism*; D009368:Neoplasm Transplantation; D012334:RNA, Neoplasm / Q000235:genetics / Q000378:metabolism*; D013274:Stomach Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology
chemical_list: D014408:Biomarkers, Tumor; D051837:COUP Transcription Factor II; D035683:MicroRNAs; D009363:Neoplasm Proteins; D012334:RNA, Neoplasm; C531388:MIRN27 microRNA, human; C095569:NR2F2 protein, human
keywords: NR2F2; gastric cancer; miR-27b; tumor metastasis