title: Direct Stimulation of Islet Insulin Secretion by Glycolytic and Mitochondrial Metabolites in KCl-Depolarized Islets.
abstract: We have previously demonstrated that islet depolarization with 70 mM KCl opens Cx36 hemichannels and allows diffusion of small metabolites and cofactors through the β-cell plasma membrane. We have investigated in this islet "permeabilized" model whether glycolytic and citric acid cycle intermediates stimulate insulin secretion and how it correlates with ATP production (islet content plus extracellular nucleotide accumulation). Glycolytic intermediates (10 mM) stimulated insulin secretion and ATP production similarly. However, they showed differential sensitivities to respiratory chain or enzyme inhibitors. Pyruvate showed a lower secretory capacity and less ATP production than phosphoenolpyruvate, implicating an important role for glycolytic generation of ATP. ATP production by glucose-6-phosphate was not sensitive to a pyruvate kinase inhibitor that effectively suppressed the phosphoenolpyruvate-induced secretory response and islet ATP rise. Strong suppression of both insulin secretion and ATP production induced by glucose-6-phosphate was caused by 10 μM antimycin A, implicating an important role for the glycerophosphate shuttle in transferring reducing equivalents to the mitochondria. Five citric acid cycle intermediates were investigated for their secretory and ATP production capacity (succinate, fumarate, malate, isocitrate and α-ketoglutarate at 5 mM, together with ADP and/or NADP+ to feed the NADPH re-oxidation cycles). The magnitude of the secretory response was very similar among the different mitochondrial metabolites but α-ketoglutarate showed a more sustained second phase of secretion. Gabaculine (1 mM, a GABA-transaminase inhibitor) suppressed the second phase of secretion and the ATP-production stimulated by α-ketoglutarate, supporting a role for the GABA shuttle in the control of glucose-induced insulin secretion. None of the other citric acid intermediates essayed showed any suppression of both insulin secretion or ATP-production by the presence of gabaculine. We propose that endogenous GABA metabolism in the "GABA-shunt" facilitates ATP production in the citric acid cycle for an optimal insulin secretion.
authors.affiliation: Obesity Research Center, Department of Medicine, Boston University School of Medicine, Boston, MA, United States of America.|Biochemistry Department, Medical School, Complutense University, Madrid, Spain.
authors.forename: Javier
authors.initials: J
authors.lastname: Pizarro-Delgado
authors.affiliation: Obesity Research Center, Department of Medicine, Boston University School of Medicine, Boston, MA, United States of America.
authors.forename: Jude T
authors.initials: JT
authors.lastname: Deeney
authors.affiliation: Obesity Research Center, Department of Medicine, Boston University School of Medicine, Boston, MA, United States of America.
authors.forename: Barbara E
authors.initials: BE
authors.lastname: Corkey
authors.affiliation: Obesity Research Center, Department of Medicine, Boston University School of Medicine, Boston, MA, United States of America.|Biochemistry Department, Medical School, Complutense University, Madrid, Spain.
authors.forename: Jorge
authors.identifier: http://orcid.org/0000-0002-6912-8745
authors.initials: J
authors.lastname: Tamarit-Rodriguez
mesh_terms: D000255:Adenosine Triphosphate / Q000096:biosynthesis; D000818:Animals; D002952:Citric Acid Cycle / Q000187:drug effects; D003509:Cyclohexanecarboxylic Acids / Q000494:pharmacology; D006019:Glycolysis / Q000187:drug effects*; D007328:Insulin / Q000378:metabolism*; D000078790:Insulin Secretion; D007515:Islets of Langerhans / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D008564:Membrane Potentials / Q000187:drug effects*; D055442:Metabolome / Q000187:drug effects*; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism*; D011189:Potassium Chloride / Q000494:pharmacology*; D017208:Rats, Wistar
chemical_list: D003509:Cyclohexanecarboxylic Acids; D007328:Insulin; C012987:gabaculine; D011189:Potassium Chloride; D000255:Adenosine Triphosphate