title: In vitrohematocrit measurement using spectrally encoded flow cytometry.
abstract: Measuring key physiological parameters of small blood samples extracted from patients could be useful for real-time clinical diagnosis at the point of care. An important parameter required from all blood tests is the blood hematocrit, a measure of the fractional volume occupied by the red cells within the blood. In this work, we present a method forin vitroevaluation of hematocrit based on the data acquired using spectrally encoded flow cytometry. Analysis of the reflectance confocal images of blood within a flow chamber resulted in an error as low as 1.7% in the measured hematocrit. The technique could be used as part of anin vitrodiagnostic system that measures important blood parameters at the point of care.
authors.affiliation: Faculty of Biomedical Engineering, Technion-Israel Institute of Technology, Haifa 3200003, Israel.
authors.forename: Adel
authors.initials: A
authors.lastname: Zeidan
authors.affiliation: Faculty of Biomedical Engineering, Technion-Israel Institute of Technology, Haifa 3200003, Israel.
authors.forename: Lior
authors.initials: L
authors.lastname: Golan
authors.affiliation: Faculty of Biomedical Engineering, Technion-Israel Institute of Technology, Haifa 3200003, Israel.
authors.forename: Dvir
authors.initials: D
authors.lastname: Yelin
keywords: (170.1470) Blood or tissue constituent monitoring; (170.1530) Cell analysis; (170.1790) Confocal microscopy