title: Changes in gene expression induced by histamine, fexofenadine and osthole: Expression of histamine H1receptor, COX-2, NF-κB, CCR1, chemokine CCL5/RANTES and interleukin-1β in PBMC allergic and non-allergic patients.
abstract: Fexofenadine (FXF) is a third-generation antihistamine drug and osthole is assumed as a natural antihistamine alternative. This paper compares results of histamine, FXF and osthole impact on HRH-1, COX-2, NF-κB-p50, CCR1 mRNA expression. We also measured mRNA expression of IL-1β and CCL5/RANTES in incubated peripheral blood mononuclear cells (PBMC) to compared how histamine, FXF and osthole had influence on expression level and interacts on product secretion.
  
  
  
  The purpose was to investigate expression pattern in asthma PBMC.
  
  
  
  The cultures were treated 72h with FXF and osthole. We measured mRNA expression of histamine HRH-1, COX-2, NF-κB-p50, CCR1, IL-1β and CCL5/RANTES with Real-Time PCR (RT-PCR).
  
  
  
  The present study suggest that osthole may be a potential inhibitor of histamine H1receptor activity. We also demonstrated that cells cultured with histamine increase COX-2 mRNA expression and osthole reduce it.
  
  
  
  Allergy remains one of the most common chronic diseases in Europe and it is rapidly approaching epidemic proportions; with current predictions estimating that the number of allergy-afflicted will equal the healthy population by 2020. It is therefore paramount to find new pharmaceuticals which successfully combat allergic disease.
authors.affiliation: University of Warmia and Mazury in Olsztyn, Olsztyn, Warmińsko-Mazurskie, Poland. Electronic address: natalia.smulska@uwm.edu.pl.
authors.forename: Natalia Karolina
authors.initials: NK
authors.lastname: Kordulewska
authors.affiliation: University of Warmia and Mazury in Olsztyn, Olsztyn, Warmińsko-Mazurskie, Poland. Electronic address: elzbieta.kostyra@uwm.edu.pl.
authors.forename: Elżbieta
authors.initials: E
authors.lastname: Kostyra
authors.affiliation: University of Warmia and Mazury in Olsztyn, Olsztyn, Warmińsko-Mazurskie, Poland. Electronic address: anna.cieslinska@uwm.edu.pl.
authors.forename: Anna
authors.initials: A
authors.lastname: Cieślińska
authors.affiliation: University of Warmia and Mazury in Olsztyn, Olsztyn, Warmińsko-Mazurskie, Poland. Electronic address: michal.matysiewicz@uwm.edu.pl.
authors.forename: Michał
authors.initials: M
authors.lastname: Matysiewicz
authors.affiliation: University of Warmia and Mazury in Olsztyn, Olsztyn, Warmińsko-Mazurskie, Poland. Electronic address: ewa.kuzbida@uwm.edu.pl.
authors.forename: Ewa
authors.initials: E
authors.lastname: Fiedorowicz
authors.affiliation: University of Warmia and Mazury in Olsztyn, Olsztyn, Warmińsko-Mazurskie, Poland. Electronic address: edyta.sienkiewicz@uwm.edu.pl.
authors.forename: Edyta
authors.initials: E
authors.lastname: Sienkiewicz-Szłapka
mesh_terms: D000328:Adult; D016022:Case-Control Studies; D018946:Chemokine CCL5 / Q000235:genetics / Q000378:metabolism; D003374:Coumarins / Q000494:pharmacology*; D051546:Cyclooxygenase 2 / Q000235:genetics / Q000378:metabolism; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects*; D006632:Histamine / Q000494:pharmacology*; D006801:Humans; D006967:Hypersensitivity / Q000235:genetics / Q000276:immunology; D053583:Interleukin-1beta / Q000235:genetics / Q000378:metabolism; D007963:Leukocytes, Mononuclear / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism*; D008297:Male; D016328:NF-kappa B / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011969:Receptors, Histamine H1 / Q000235:genetics / Q000378:metabolism; D016593:Terfenadine / Q000031:analogs & derivatives* / Q000494:pharmacology
chemical_list: D018946:Chemokine CCL5; D003374:Coumarins; D053583:Interleukin-1beta; D016328:NF-kappa B; D012333:RNA, Messenger; D011969:Receptors, Histamine H1; D016593:Terfenadine; D006632:Histamine; C093230:fexofenadine; D051546:Cyclooxygenase 2; C046627:osthol
keywords: Allergic disease; Antihistamine drugs; Cultures in vitro; Expression in PBMCs