title: Reactive Carbonyl Species Mediate ABA Signaling in Guard Cells.
abstract: Drought is responsible for a massive reduction in crop yields. In response to drought, plants synthesize the hormone ABA, which induces stomatal closure, thus reducing water loss. In guard cells, ABA triggers production of reactive oxygen species (ROS), which is mediated by NAD(P)H oxidases. The production of ROS is a key factor for ABA-induced stomatal closure, but it remains to be clarified how the production of ROS is transduced into downstream signaling components in guard cells. We investigated roles of reactive carbonyl species (RCS) in ABA-induced stomatal closure using transgenic tobacco (Nicotiana tabacum) overexpressing Arabidopsis 2-alkenal reductase (AER-OE), which scavenges RCS. ABA and hydrogen peroxide (H2O2) induced accumulation of RCS including acrolein and 4-hydroxy-(E)-2-nonenal in wild-type tobacco but not in AER-OE. Stomatal closure and RCS accumulation in response to ABA and H2O2were inhibited in AER-OE unlike in the wild type, while ABA-induced H2O2production in guard cells was observed in AER-OE as well as in the wild type. Moreover, ABA inhibited inward-rectifying K+channels in wild-type guard cells but not in AER-OE guard cells. These results suggest that RCS is involved in ABA-induced stomatal closure and functions downstream of H2O2production in the ABA signaling pathway in guard cells.
authors.affiliation: Graduate School of Environmental and Life Science, Okayama University, 1-1-1 Tsushima-Naka, Okayama, 700-8530 Japan.|Department of Agronomy, Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur 1706, Bangladesh.|These authors contributed equally to this work.
authors.forename: Md Moshiul
authors.initials: MM
authors.lastname: Islam
authors.affiliation: Graduate School of Environmental and Life Science, Okayama University, 1-1-1 Tsushima-Naka, Okayama, 700-8530 Japan.|These authors contributed equally to this work.
authors.forename: Wenxiu
authors.initials: W
authors.lastname: Ye
authors.affiliation: Graduate School of Environmental and Life Science, Okayama University, 1-1-1 Tsushima-Naka, Okayama, 700-8530 Japan.
authors.forename: Daiki
authors.initials: D
authors.lastname: Matsushima
authors.affiliation: Graduate School of Environmental and Life Science, Okayama University, 1-1-1 Tsushima-Naka, Okayama, 700-8530 Japan.
authors.forename: Shintaro
authors.initials: S
authors.lastname: Munemasa
authors.affiliation: Graduate School of Environmental and Life Science, Okayama University, 1-1-1 Tsushima-Naka, Okayama, 700-8530 Japan.
authors.forename: Eiji
authors.initials: E
authors.lastname: Okuma
authors.affiliation: Graduate School of Environmental and Life Science, Okayama University, 1-1-1 Tsushima-Naka, Okayama, 700-8530 Japan.
authors.forename: Yoshimasa
authors.initials: Y
authors.lastname: Nakamura
authors.affiliation: Graduate School of Agriculture, Yamaguchi University, Yoshida 1677-1, Yamaguchi, 753-8515 Japan.
authors.forename: Sanaullah
authors.initials: S
authors.lastname: Biswas
authors.affiliation: Graduate School of Agriculture, Yamaguchi University, Yoshida 1677-1, Yamaguchi, 753-8515 Japan.|Science Research Center, Yamaguchi University, Yoshida 1677-1, Yamaguchi, 753-8515 Japan.
authors.forename: Jun'ichi
authors.initials: J
authors.lastname: Mano
authors.affiliation: Graduate School of Environmental and Life Science, Okayama University, 1-1-1 Tsushima-Naka, Okayama, 700-8530 Japan muta@cc.okayama-u.ac.jp.
authors.forename: Yoshiyuki
authors.initials: Y
authors.lastname: Murata
mesh_terms: D000040:Abscisic Acid / Q000378:metabolism*; D017360:Arabidopsis / Q000235:genetics*; D029681:Arabidopsis Proteins / Q000235:genetics; D055864:Droughts; D005609:Free Radicals / Q000032:analysis / Q000378:metabolism*; D015870:Gene Expression; D006861:Hydrogen Peroxide / Q000032:analysis / Q000378:metabolism; D019255:NADPH Oxidases / Q000235:genetics / Q000378:metabolism; D010937:Plant Growth Regulators / Q000378:metabolism*; D054046:Plant Stomata / Q000235:genetics / Q000502:physiology; D030821:Plants, Genetically Modified; D017382:Reactive Oxygen Species / Q000032:analysis / Q000378:metabolism; D015398:Signal Transduction; D014026:Nicotiana / Q000201:enzymology / Q000235:genetics / Q000502:physiology*
chemical_list: D000040:Abscisic Acid; D029681:Arabidopsis Proteins; D005609:Free Radicals; D006861:Hydrogen Peroxide; D019255:NADPH Oxidases; D010937:Plant Growth Regulators; D017382:Reactive Oxygen Species; C000615822:AER protein, Arabidopsis
keywords: ABA; Reactive carbonyl species; Reactive oxygen species; Stomatal closure