title: Role of Pea Enation Mosaic Virus Coat Protein in the Host Plant and Aphid Vector.
abstract: Understanding the molecular mechanisms involved in plant virus-vector interactions is essential for the development of effective control measures for aphid-vectored epidemic plant diseases. The coat proteins (CP) are the main component of the viral capsids, and they are implicated in practically every stage of the viral infection cycle.Pea enation mosaic virus1 (PEMV1,Enamovirus,Luteoviridae) andPea enation mosaic virus2 (PEMV2,Umbravirus,Tombusviridae) are two RNA viruses in an obligate symbiosis causing the pea enation mosaic disease. Sixteen mutant viruses were generated with mutations in different domains of the CP to evaluate the role of specific amino acids in viral replication, virion assembly, long-distance movement inPisum sativum, and aphid transmission. Twelve mutant viruses were unable to assemble but were able to replicate in inoculated leaves, move long-distance, and express the CP in newly infected leaves. Four mutant viruses produced virions, but three were not transmissible by the pea aphid,Acyrthosiphon pisum. Three-dimensional modeling of the PEMV CP, combined with biological assays for virion assembly and aphid transmission, allowed for a model of the assembly of PEMV coat protein subunits.
authors.affiliation: Department of Plant Pathology & Microbiology, 351 Bessey Hall, Iowa State University, Ames, IA 50011, USA. juliette.doumayrou@gmail.com.
authors.forename: Juliette
authors.initials: J
authors.lastname: Doumayrou
authors.affiliation: Department of Plant Pathology & Microbiology, 351 Bessey Hall, Iowa State University, Ames, IA 50011, USA. mksheber@iastate.edu.
authors.forename: Melissa
authors.initials: M
authors.lastname: Sheber
authors.affiliation: Department of Entomology, 339 Science II, Iowa State University, Ames, IA 50011, USA. bbonning@iastate.edu.
authors.forename: Bryony C
authors.initials: BC
authors.lastname: Bonning
authors.affiliation: Department of Plant Pathology & Microbiology, 351 Bessey Hall, Iowa State University, Ames, IA 50011, USA. wamiller@iastate.edu.
authors.forename: W Allen
authors.initials: WA
authors.lastname: Miller
mesh_terms: D000818:Animals; D001042:Aphids / Q000821:virology*; D036022:Capsid Proteins / Q000235:genetics / Q000378:metabolism*; D004252:DNA Mutational Analysis; D054884:Host-Pathogen Interactions; D052679:Luteoviridae / Q000235:genetics / Q000502:physiology*; D018532:Pisum sativum / Q000821:virology*; D018515:Plant Leaves / Q000821:virology; D019183:Tombusviridae / Q000235:genetics / Q000502:physiology*; D019065:Virus Assembly; D014779:Virus Replication
chemical_list: D036022:Capsid Proteins
keywords: Pea enation mosaic virus; aphid transmission; coat protein; viral accumulation; viral encapsidation