title: Error-Correcting Output Codes in Classification of Human Induced Pluripotent Stem Cell Colony Images.
abstract: The purpose of this paper is to examine how well the human induced pluripotent stem cell (hiPSC) colony images can be classified using error-correcting output codes (ECOC). Our image dataset includes hiPSC colony images from three classes (bad, semigood, and good) which makes our classification task a multiclass problem. ECOC is a general framework to model multiclass classification problems. We focus on four different coding designs of ECOC and apply to each one of themk-Nearest Neighbor (k-NN) searching, naïve Bayes, classification tree, and discriminant analysis variants classifiers. We use Scaled Invariant Feature Transformation (SIFT) based features in classification. The best accuracy (62.4%) is obtained with ternary complete ECOC coding design andk-NN classifier (standardized Euclidean distance measure and inverse weighting). The best result is comparable with our earlier research. The quality identification of hiPSC colony images is an essential problem to be solved before hiPSCs can be used in practice in large-scale. ECOC methods examined are promising techniques for solving this challenging problem.
authors.affiliation: School of Information Sciences, University of Tampere, Kanslerinrinne 1, 33014 Tampere, Finland.
authors.forename: Henry
authors.identifier: 0000-0001-6341-7055
authors.initials: H
authors.lastname: Joutsijoki
authors.affiliation: BioMediTech, University of Tampere, Biokatu 12, 33520 Tampere, Finland.
authors.forename: Markus
authors.initials: M
authors.lastname: Haponen
authors.affiliation: School of Information Sciences, University of Tampere, Kanslerinrinne 1, 33014 Tampere, Finland.
authors.forename: Jyrki
authors.initials: J
authors.lastname: Rasku
authors.affiliation: School of Medicine, University of Tampere, Biokatu 12, 33520 Tampere, Finland.
authors.forename: Katriina
authors.identifier: 0000-0003-0334-2172
authors.initials: K
authors.lastname: Aalto-Setälä
authors.affiliation: School of Information Sciences, University of Tampere, Kanslerinrinne 1, 33014 Tampere, Finland.
authors.forename: Martti
authors.identifier: 0000-0003-2298-9553
authors.initials: M
authors.lastname: Juhola
mesh_terms: D002454:Cell Differentiation / Q000235:genetics*; D058948:Cell Tracking / Q000379:methods; D065150:Cellular Reprogramming / Q000235:genetics; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000145:classification / Q000648:ultrastructure*; D057054:Molecular Imaging; D013234:Stem Cells / Q000145:classification / Q000648:ultrastructure*