title: Protein Expression Differences of 2-Dimensional and Progressive 3-Dimensional Cell Cultures of Non-Small-Cell-Lung-Cancer Cell Line H460.
abstract: Non-small-cell-lung-cancer (NSCLC) constitutes about 75-80% of lung cancers. The challenge to tackle cancers is in early diagnosis and arriving at safer therapeutic options. In vitro studies using cancer cell lines continue to contribute significantly in understanding cancers. Cell culture methods have evolved and the recent developments in 3 dimensional (3D) cell cultures are inducing greater resemblance of the in vitro cultured cells with in vivo conditions. In this study, we established 3D aggregates of H460 cell line on agarose hydrogels and studied the protein expression differences among cells grown as monolayers (2D) and the progressively developing 3D aggregates from days 2 to 10. Analysis included matching of those proteins expressed by the developing aggregates and the available literature on progressing tumors in vivo. J. Cell. Biochem. 118: 1648-1652, 2017. © 2016 Wiley Periodicals, Inc.
authors.affiliation: Faculty of Biomedical Sciences, Department of Human Genetics, Sri Ramachandra University, Porur, Chennai, Tamil Nadu, 600116, India.
authors.forename: Maddaly
authors.initials: M
authors.lastname: Ravi
authors.affiliation: Faculty of Biomedical Sciences, Department of Human Genetics, Sri Ramachandra University, Porur, Chennai, Tamil Nadu, 600116, India.
authors.forename: Divya K
authors.initials: DK
authors.lastname: Mohan
authors.affiliation: Faculty of Biomedical Sciences, Department of Human Genetics, Sri Ramachandra University, Porur, Chennai, Tamil Nadu, 600116, India.
authors.forename: Bellona
authors.initials: B
authors.lastname: Sahu
mesh_terms: D014408:Biomarkers, Tumor / Q000378:metabolism*; D002289:Carcinoma, Non-Small-Cell Lung / Q000378:metabolism*; D018929:Cell Culture Techniques; D045744:Cell Line, Tumor; D004591:Electrophoresis, Polyacrylamide Gel; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics / Q000502:physiology; D006801:Humans; D008175:Lung Neoplasms / Q000378:metabolism
chemical_list: D014408:Biomarkers, Tumor
keywords: 3D AGGREGATES; H460 CELLS; PROTEIN CANCER BIOMARKERS; PROTEIN EXPRESSION; PROTEIN EXTRACTS; SDS-PAGE ANALYSIS