title: Cancer biomarker sensing using packaged plasmonic optical fiber gratings: Towards in vivo diagnosis.
abstract: This work presents the development of an innovative plasmonic optical fiber (OF) immunosensor for the detection of cytokeratin 17 (CK17), a biomarker of interest for lung cancer diagnosis. The development of this sensing platform is such that it can be assessed in non-liquid environments, demonstrating that a surface plasmon resonance (SPR) can be excited in this case. For this purpose, detections have been first carried out on CK17 encapsulated in gel matrix in the aim of mimicking tissue samples. Gold-coated OF immunosensors were embedded in a specifically designed packaging providing enough stiffness to penetrate into soft matters. Resulting reflected spectra have revealed, for the first time, the presence of a stable SPR signal recorded in soft matters. Experiments conducted to detect CK17 trapped in a porous polyacrylamide gel matrix have highlighted the specific and selective biosensor response towards the target protein. Finally, the packaged OF immunosensor has been validated by a preliminary test on human lung biopsy, which has confirmed the ex-vivo CK17 detection. Consequently, this work represents an important milestone towards the detection of biomarkers in tissues, which is still a clinical challenge for minimally-invasive in vivo medical diagnosis.
authors.affiliation: Proteomics and Microbiology Department, Université de Mons, 20 place du Parc, 7000 Mons, Belgium; Electromagnetism and Telecommunication Department, Université de Mons, Bld Dolez 31, 7000 Mons, Belgium.
authors.forename: Clotilde
authors.initials: C
authors.lastname: Ribaut
authors.affiliation: Proteomics and Microbiology Department, Université de Mons, 20 place du Parc, 7000 Mons, Belgium.
authors.forename: Médéric
authors.initials: M
authors.lastname: Loyez
authors.affiliation: Bio, Electro And Mechanical System Department (CP 165/56), Université libre de Bruxelles, Av. F.D. Roosevelt, 50 B-1050 Brussels, Belgium.
authors.forename: Jean-Charles
authors.initials: JC
authors.lastname: Larrieu
authors.affiliation: Proteomics and Microbiology Department, Université de Mons, 20 place du Parc, 7000 Mons, Belgium.
authors.forename: Samia
authors.initials: S
authors.lastname: Chevineau
authors.affiliation: Bio, Electro And Mechanical System Department (CP 165/56), Université libre de Bruxelles, Av. F.D. Roosevelt, 50 B-1050 Brussels, Belgium.
authors.forename: Pierre
authors.initials: P
authors.lastname: Lambert
authors.affiliation: Department of Pathology, Université Libre de Bruxelles, Hôpital Erasme, 808 Route de Lennik, 1070 Brussels, Belgium.
authors.forename: Myriam
authors.initials: M
authors.lastname: Remmelink
authors.affiliation: Proteomics and Microbiology Department, Université de Mons, 20 place du Parc, 7000 Mons, Belgium.
authors.forename: Ruddy
authors.initials: R
authors.lastname: Wattiez
authors.affiliation: Electromagnetism and Telecommunication Department, Université de Mons, Bld Dolez 31, 7000 Mons, Belgium. Electronic address: christophe.caucheteur@umons.ac.be.
authors.forename: Christophe
authors.initials: C
authors.lastname: Caucheteur
mesh_terms: D055910:Antibodies, Immobilized / Q000737:chemistry; D014408:Biomarkers, Tumor / Q000032:analysis; D004867:Equipment Design; D005336:Fiber Optic Technology / Q000295:instrumentation; D006046:Gold / Q000737:chemistry; D006801:Humans; D007118:Immunoassay / Q000295:instrumentation; D053537:Keratin-17 / Q000032:analysis*; D008168:Lung / Q000473:pathology*; D008175:Lung Neoplasms / Q000175:diagnosis*; D055100:Optical Fibers; D020349:Surface Plasmon Resonance / Q000295:instrumentation*
chemical_list: D055910:Antibodies, Immobilized; D014408:Biomarkers, Tumor; D053537:Keratin-17; D006046:Gold
keywords: Biomarkers detection in soft matters; Catheter and mechanical packaging; Cytokeratin 17 (CK17) detection; Fiber optic biosensor; Surface plasmon resonance (SPR); Tilted fiber Bragg grating (TFBG)