title: Cytocompatible Polymer Grafting from Individual Living Cells by Atom-Transfer Radical Polymerization.
abstract: A cytocompatible method of surface-initiated, activator regenerated by electron transfer, atom transfer radical polymerization (SI-ARGET ATRP) is developed for engineering cell surfaces with synthetic polymers. Dopamine-based ATRP initiators are used for both introducing the ATRP initiator onto chemically complex cell surfaces uniformly (by the material-independent coating property of polydopamine) and protecting the cells from radical attack during polymerization (by the radical-scavenging property of polydopamine). Synthetic polymers are grafted onto the surface of individual yeast cells without significant loss of cell viability, and the uniform and dense grafting is confirmed by various characterization methods including agglutination assay and cell-division studies. This work will provide a strategic approach to the generation of living cell-polymer hybrid structures and open the door to their application in multitude of areas, such as sensor technology, catalysis, theranostics, and cell therapy.
authors.affiliation: Center for Cell-Encapsulation Research, Department of Chemistry, KAIST, Daejeon, 34141, Korea.
authors.forename: Ji Yup
authors.initials: JY
authors.lastname: Kim
authors.affiliation: Center for Cell-Encapsulation Research, Department of Chemistry, KAIST, Daejeon, 34141, Korea.
authors.forename: Bong Soo
authors.initials: BS
authors.lastname: Lee
authors.affiliation: Department of Chemistry Education, Korea National University of Education, Chungbuk, 28173, Korea.
authors.forename: Jinsu
authors.initials: J
authors.lastname: Choi
authors.affiliation: Center for Cell-Encapsulation Research, Department of Chemistry, KAIST, Daejeon, 34141, Korea.
authors.forename: Beom Jin
authors.initials: BJ
authors.lastname: Kim
authors.affiliation: Center for Cell-Encapsulation Research, Department of Chemistry, KAIST, Daejeon, 34141, Korea.
authors.forename: Ji Yu
authors.initials: JY
authors.lastname: Choi
authors.affiliation: Department of Chemistry, Chungbuk National University, Cheongju, 28644, Korea.
authors.forename: Sung Min
authors.initials: SM
authors.lastname: Kang
authors.affiliation: Department of Chemistry Education, Korea National University of Education, Chungbuk, 28173, Korea.
authors.forename: Sung Ho
authors.initials: SH
authors.lastname: Yang
authors.affiliation: Center for Cell-Encapsulation Research, Department of Chemistry, KAIST, Daejeon, 34141, Korea.
authors.forename: Insung S
authors.initials: IS
authors.lastname: Choi
mesh_terms: D002470:Cell Survival; D005609:Free Radicals / Q000138:chemical synthesis / Q000737:chemistry; D058105:Polymerization; D011108:Polymers / Q000138:chemical synthesis / Q000737:chemistry*; D012441:Saccharomyces cerevisiae / Q000166:cytology*
chemical_list: D005609:Free Radicals; D011108:Polymers
keywords: cell surface engineering; polydopamine; polymer brushes; radical reactions; surface chemistry