title: Association of SCNN1B promoter methylation with essential hypertension.
abstract: The amiloride-sensitive sodium channel beta subunit (SCNN1B) gene encodes the beta subunit of the epithelial sodium channel, which is involved in blood pressure homeostasis. The aim of the present study was to investigate the association between SCNN1B gene promoter methylation and essential hypertension (EH), and to explore whether SCNN1B methylation was altered by antihypertensive therapy. The present study recruited 282 individuals: 94 controls, 94 incident cases and 94 prevalent cases. Subsequently, the methylation status of six CpG sites in the SCNN1B promoter region was measured using bisulfite pyrosequencing technology. Among the six CpG sites, a significant difference in CpG1 and CpG2 methylation levels were detected between controls and incident cases (CpG1: β‑standardized=0.17, adjusted P=0.015; CpG2: β‑standardized=‑0.41, adjusted P=0.001). In addition, a significant difference was detected in CpG1 methylation levels between incident cases and prevalent cases (β‑standardized=‑0.252, adjusted P=3.77E‑04). The present study also demonstrated that CpG1 and CpG2 methylation levels were significantly lower in males compared with in females (CpG1: t=‑3.180, P=0.002; CpG2: t=‑2.148, P=0.033). CpG1 methylation was also shown to be positively correlated with age (controls: r=0.285, P=0.008; incident cases: r=0.401, P=0.0001; prevalent cases: r=0.367, P=0.001). These results indicated a significant association between EH and SCNN1B methylation, which was affected by age, gender and antihypertensive therapy.
authors.affiliation: Zhejiang Provincial Key Laboratory of Pathophysiology, School of Medicine, Ningbo University, Ningbo, Zhejiang 315211, P.R. China.
authors.forename: Qilong
authors.initials: Q
authors.lastname: Zhong
authors.affiliation: Clinical Laboratory, Ningbo Baizhang Street Community Health Service Center, Ningbo, Zhejiang 315200, P.R. China.
authors.forename: Chunyan
authors.initials: C
authors.lastname: Liu
authors.affiliation: Zhejiang Provincial Key Laboratory of Pathophysiology, School of Medicine, Ningbo University, Ningbo, Zhejiang 315211, P.R. China.
authors.forename: Rui
authors.initials: R
authors.lastname: Fan
authors.affiliation: Zhejiang Provincial Key Laboratory of Pathophysiology, School of Medicine, Ningbo University, Ningbo, Zhejiang 315211, P.R. China.
authors.forename: Shiwei
authors.initials: S
authors.lastname: Duan
authors.affiliation: Zhejiang Provincial Key Laboratory of Pathophysiology, School of Medicine, Ningbo University, Ningbo, Zhejiang 315211, P.R. China.
authors.forename: Xuting
authors.initials: X
authors.lastname: Xu
authors.affiliation: Zhejiang Provincial Key Laboratory of Pathophysiology, School of Medicine, Ningbo University, Ningbo, Zhejiang 315211, P.R. China.
authors.forename: Jinshun
authors.initials: J
authors.lastname: Zhao
authors.affiliation: Zhejiang Provincial Key Laboratory of Pathophysiology, School of Medicine, Ningbo University, Ningbo, Zhejiang 315211, P.R. China.
authors.forename: Shuqi
authors.initials: S
authors.lastname: Mao
authors.affiliation: Zhejiang Provincial Key Laboratory of Pathophysiology, School of Medicine, Ningbo University, Ningbo, Zhejiang 315211, P.R. China.
authors.forename: Wen
authors.initials: W
authors.lastname: Zhu
authors.affiliation: Clinical laboratory, The Seventh Hospital of Ningbo, Ningbo, Zhejiang 315202, P.R. China.
authors.forename: Lingmei
authors.initials: L
authors.lastname: Hao
authors.affiliation: Clinical Laboratory, The First Hospital of Ningbo, Ningbo, Zhejiang 315010, P.R. China.
authors.forename: Fengying
authors.initials: F
authors.lastname: Yin
authors.affiliation: Zhejiang Provincial Key Laboratory of Pathophysiology, School of Medicine, Ningbo University, Ningbo, Zhejiang 315211, P.R. China.
authors.forename: Lina
authors.initials: L
authors.lastname: Zhang
mesh_terms: D000959:Antihypertensive Agents / Q000627:therapeutic use; D018899:CpG Islands; D019175:DNA Methylation; D053503:Epithelial Sodium Channels / Q000235:genetics*; D000075222:Essential Hypertension; D005260:Female; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease; D006801:Humans; D006973:Hypertension / Q000188:drug therapy / Q000235:genetics* / Q000503:physiopathology; D008297:Male; D016017:Odds Ratio; D011401:Promoter Regions, Genetic; D012307:Risk Factors
chemical_list: D000959:Antihypertensive Agents; D053503:Epithelial Sodium Channels; C507048:SCNN1B protein, human