title: Sex-specific mitonuclear epistasis and the evolution of mitochondrial bioenergetics, ageing, and life history in seed beetles.
abstract: The role of mitochondrial DNA for the evolution of life-history traits remains debated. We examined mitonuclear effects on the activity of the multisubunit complex of the electron transport chain (ETC) involved in oxidative phosphorylation (OXPHOS) across lines of the seed beetle Acanthoscelides obtectus selected for a short (E) or a long (L) life for more than >160 generations. We constructed and phenotyped mitonuclear introgression lines, which allowed us to assess the independent effects of the evolutionary history of the nuclear and the mitochondrial genome. The nuclear genome was responsible for the largest share of divergence seen in ageing. However, the mitochondrial genome also had sizeable effects, which were sex-specific and expressed primarily as epistatic interactions with the nuclear genome. The effects of mitonuclear disruption were largely consistent with mitonuclear coadaptation. Variation in ETC activity explained a large proportion of variance in ageing and life-history traits and this multivariate relationship differed somewhat between the sexes. In conclusion, mitonuclear epistasis has played an important role in the laboratory evolution of ETC complex activity, ageing, and life histories and these are closely associated. The mitonuclear architecture of evolved differences in life-history traits and mitochondrial bioenergetics was sex-specific.
authors.affiliation: Department of Evolutionary Biology, Institute for Biological Research, University of Belgrade, Despota Stefana Boulevard 142, Belgrade, 11060, Serbia.
authors.forename: Mirko
authors.initials: M
authors.lastname: Đorđević
authors.affiliation: Department of Evolutionary Biology, Institute for Biological Research, University of Belgrade, Despota Stefana Boulevard 142, Belgrade, 11060, Serbia.|Institute of Zoology, Faculty of Biology, University of Belgrade, Studentski trg 16, Belgrade, 11000, Serbia.
authors.forename: Biljana
authors.initials: B
authors.lastname: Stojković
authors.affiliation: Department of Evolutionary Biology, Institute for Biological Research, University of Belgrade, Despota Stefana Boulevard 142, Belgrade, 11060, Serbia.
authors.forename: Uroš
authors.initials: U
authors.lastname: Savković
authors.affiliation: Animal Ecology, Department of Ecology and Genetics, Evolutionary Biology Centre, Uppsala University, Norbyvägen 18D, SE-752 36, Uppsala, Sweden.
authors.forename: Elina
authors.initials: E
authors.lastname: Immonen
authors.affiliation: Department of Evolutionary Biology, Institute for Biological Research, University of Belgrade, Despota Stefana Boulevard 142, Belgrade, 11060, Serbia.
authors.forename: Nikola
authors.initials: N
authors.lastname: Tucić
authors.affiliation: Department of Insect Physiology and Biochemistry, Institute for Biological Research, University of Belgrade, Despota Stefana Boulevard 142, Belgrade, 11060, Serbia.
authors.forename: Jelica
authors.initials: J
authors.lastname: Lazarević
authors.affiliation: Animal Ecology, Department of Ecology and Genetics, Evolutionary Biology Centre, Uppsala University, Norbyvägen 18D, SE-752 36, Uppsala, Sweden.
authors.forename: Göran
authors.initials: G
authors.lastname: Arnqvist
mesh_terms: D000375:Aging; D000818:Animals; D001517:Coleoptera / Q000235:genetics / Q000502:physiology*; D045222:Electron Transport Chain Complex Proteins / Q000235:genetics / Q000378:metabolism; D004734:Energy Metabolism; D004843:Epistasis, Genetic; D005260:Female; D019476:Insect Proteins / Q000235:genetics / Q000378:metabolism; D000071421:Life History Traits; D008297:Male; D008928:Mitochondria / Q000235:genetics*; D012641:Selection, Genetic
chemical_list: D045222:Electron Transport Chain Complex Proteins; D019476:Insect Proteins
keywords: Bruchinae; OXPHOS; coadaptation; epistasis; evolution of ageing; mitochondria; mtDNA; senescence; sexual dimorphism