title: Cellular delivery of enzyme-loaded DNA origami.
abstract: In this communication, we show that active enzymes can be delivered into HEK293 cells in vitro when they are attached to tubular DNA origami nanostructures. We use bioluminescent enzymes as a cargo and monitor their activity from a cell lysate. The results show that the enzymes stay intact and retain their activity in the transfection process. The method is highly modular, which makes it a compelling candidate for a great variety of delivery applications.
authors.affiliation: Biohybrid Materials, Department of Biotechnology and Chemical Technology, Aalto University, P. O. Box 16100, FI-00076 Aalto, Finland. mauri.kostiainen@aalto.fi veikko.linko@aalto.fi.
authors.forename: Ari
authors.initials: A
authors.lastname: Ora
authors.affiliation: Biohybrid Materials, Department of Biotechnology and Chemical Technology, Aalto University, P. O. Box 16100, FI-00076 Aalto, Finland. mauri.kostiainen@aalto.fi veikko.linko@aalto.fi.
authors.forename: Erika
authors.initials: E
authors.lastname: Järvihaavisto
authors.affiliation: Division of Pharmaceutical Chemistry and Technology, Faculty of Pharmacy, University of Helsinki, FI-00790 Helsinki, Finland.
authors.forename: Hongbo
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Biohybrid Materials, Department of Biotechnology and Chemical Technology, Aalto University, P. O. Box 16100, FI-00076 Aalto, Finland. mauri.kostiainen@aalto.fi veikko.linko@aalto.fi.
authors.forename: Henni
authors.initials: H
authors.lastname: Auvinen
authors.affiliation: Division of Pharmaceutical Chemistry and Technology, Faculty of Pharmacy, University of Helsinki, FI-00790 Helsinki, Finland.
authors.forename: Hélder A
authors.identifier: http://orcid.org/0000-0001-7850-6309
authors.initials: HA
authors.lastname: Santos
authors.affiliation: Biohybrid Materials, Department of Biotechnology and Chemical Technology, Aalto University, P. O. Box 16100, FI-00076 Aalto, Finland. mauri.kostiainen@aalto.fi veikko.linko@aalto.fi.
authors.forename: Mauri A
authors.initials: MA
authors.lastname: Kostiainen
authors.affiliation: Biohybrid Materials, Department of Biotechnology and Chemical Technology, Aalto University, P. O. Box 16100, FI-00076 Aalto, Finland. mauri.kostiainen@aalto.fi veikko.linko@aalto.fi.
authors.forename: Veikko
authors.identifier: http://orcid.org/0000-0003-2762-1555
authors.initials: V
authors.lastname: Linko
mesh_terms: D004247:DNA / Q000737:chemistry / Q000378:metabolism*; D057809:HEK293 Cells; D006801:Humans; D008156:Luciferases / Q000737:chemistry / Q000378:metabolism*; D049449:Luminescence; D049329:Nanostructures / Q000737:chemistry
chemical_list: D004247:DNA; D008156:Luciferases