title: Decreased percentage of NKG2D+NK cells in patients with incident onset of Type 1 Diabetes.
abstract: Type 1 diabetes mellitus (T1DM) is characterized by absolute insulin deficiency owing to autoimmune destruction of the pancreatic β cells. A significant decrease in natural killer (NK) cells in peripheral blood has been observed in patients with untreated T1DM. In the present study, we aimed to explore the role of NK cells and their subsets in young T1DM patients. A total of 30 children and adolescents with untreated T1DM and 27 healthy controls (HC) were recruited in this study. Flow cytometry analysis indicated that the percentage of peripheral blood CD3-CD56+ NK cells and NK cells subsets (CD56bright, CD56dim and CD56neg), were significantly decreased in the T1DM patients compared to healthy controls. In addition, the percentage of inducible CD107a+ and IFN-γ-secreting NK cells was significantly decreased compared to HC. Interestingly, the percentage of NKG2D+ NK cells negatively correlated with the level of serum TCHOL and TG in T1DM patients. Our data indicate that decreased number and impaired function of NK cells may have a role in the pathogenesis of T1DM.
authors.affiliation: Department of Central Laboratory, The First Hospital, Jilin University, Changchun, China.|Department of Endocrinology, Weihai Municipal Hospital, Weihai, China.
authors.forename: Yupan
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Central Laboratory, The First Hospital, Jilin University, Changchun, China.
authors.forename: Haifeng
authors.initials: H
authors.lastname: Wang
authors.affiliation: Department of Central Laboratory, The First Hospital, Jilin University, Changchun, China.
authors.forename: Xiaoqian
authors.initials: X
authors.lastname: Lou
authors.affiliation: Department of Central Laboratory, The First Hospital, Jilin University, Changchun, China.
authors.forename: Xiaozhang
authors.initials: X
authors.lastname: Qu
authors.affiliation: Department of Central Laboratory, The First Hospital, Jilin University, Changchun, China.
authors.forename: Lichao
authors.initials: L
authors.lastname: Gao
authors.affiliation: Key Laboratory of Zoonosis Research, Ministry of Education, The First Hospital, Jilin University, Changchun, China.
authors.forename: Xiaolei
authors.initials: X
authors.lastname: Liu
authors.affiliation: Department of Central Laboratory, The First Hospital, Jilin University, Changchun, China.
authors.forename: Man
authors.initials: M
authors.lastname: Li
authors.affiliation: Department of Central Laboratory, The First Hospital, Jilin University, Changchun, China.|Department of Endocrinology, Weihai Municipal Hospital, Weihai, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Guo
authors.affiliation: Department of Central Laboratory, The First Hospital, Jilin University, Changchun, China.|Key Laboratory of Zoonosis Research, Ministry of Education, The First Hospital, Jilin University, Changchun, China.|Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, China.
authors.forename: Yanfang
authors.initials: Y
authors.lastname: Jiang
mesh_terms: D000293:Adolescent; D016022:Case-Control Studies; D015550:Cell Degranulation; D002648:Child; D016207:Cytokines; D003922:Diabetes Mellitus, Type 1 / Q000097:blood / Q000276:immunology*; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D007371:Interferon-gamma / Q000378:metabolism; D007694:Killer Cells, Natural / Q000276:immunology* / Q000378:metabolism / Q000502:physiology; D008297:Male; D055655:NK Cell Lectin-Like Receptor Subfamily K / Q000378:metabolism*
chemical_list: D016207:Cytokines; C527085:KLRK1 protein, human; D055655:NK Cell Lectin-Like Receptor Subfamily K; D007371:Interferon-gamma
keywords: KIR2DL3; NKG2A; NKG2D; natural killer cells; type 1 diabetes mellitus