title: Development and characterization of microsatellite loci forLindera glauca(Lauraceae).
abstract: OBJECTIVE
  Microsatellite primers were developed to investigate population genetic structure inLindera glauca(Lauraceae).
  
  
  RESULTS
  Twenty-five microsatellite primers were developed and optimized forL. glaucausing Illumina's Solexa sequencing technology. These novel primers were found to be polymorphic in nine wildL. glaucapopulations with 81 total alleles confirmed and genotyped via capillary gel electrophoresis. The total number of alleles, observed heterozygosity, and expected heterozygosity for each population ranged from one to four, from 0.00 to 0.90, and from 0.00 to 0.79, respectively. In addition, the 25 primers were tested in 10 additional individuals of the related speciesL. communis, and all but four markers showed good amplification results.
  
  
  CONCLUSIONS
  This set of microsatellite primers is the first specifically developed forL. glaucaand will facilitate studies of genetic diversity and evolution among populations of this species.
authors.affiliation: Laboratory of Systematic Evolution and Biogeography of Woody Plants, School of Nature Conservation, Beijing Forestry University, 35 Qinghua East Road, Beijing 100083, People's Republic of China.
authors.forename: Biao
authors.initials: B
authors.lastname: Xiong
authors.affiliation: Laboratory of Systematic Evolution and Biogeography of Woody Plants, School of Nature Conservation, Beijing Forestry University, 35 Qinghua East Road, Beijing 100083, People's Republic of China.
authors.forename: Shubin
authors.initials: S
authors.lastname: Dong
authors.affiliation: Laboratory of Systematic Evolution and Biogeography of Woody Plants, School of Nature Conservation, Beijing Forestry University, 35 Qinghua East Road, Beijing 100083, People's Republic of China.
authors.forename: Ji
authors.initials: J
authors.lastname: Qi
authors.affiliation: Laboratory of Systematic Evolution and Biogeography of Woody Plants, School of Nature Conservation, Beijing Forestry University, 35 Qinghua East Road, Beijing 100083, People's Republic of China.
authors.forename: Limei
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Jigongshan National Nature Reserve, Xinyang 464133, People's Republic of China.
authors.forename: Denglong
authors.initials: D
authors.lastname: Ha
authors.affiliation: Jigongshan National Nature Reserve, Xinyang 464133, People's Republic of China.
authors.forename: Yuxi
authors.initials: Y
authors.lastname: Ju
authors.affiliation: Laboratory of Systematic Evolution and Biogeography of Woody Plants, School of Nature Conservation, Beijing Forestry University, 35 Qinghua East Road, Beijing 100083, People's Republic of China.
authors.forename: Zhixiang
authors.initials: Z
authors.lastname: Zhang
keywords: Lauraceae; Lindera glauca; genetic diversity; microsatellite; polymorphism