title: Long non‑coding RNA AK055347 is upregulated in patients with atrial fibrillation and regulates mitochondrial energy production in myocardiocytes.
abstract: The role of long non-coding RNAs (lncRNAs) in atrial fibrillation remains to be fully elucidated. The current study performed microarray analysis to investigate differential lncRNA expression profiling in atrial samples from the pulmonary vein and the surrounding left atrial area (LA‑PV) and from the left atrial appendage (LAA) in 16 patients with atrial fibrillation (AF). Microarray analysis identified 94 lncRNAs that were differentially expressed between the LA‑PV and LAA in patients with AF. AK055347 was one of lncRNAs with the most significant alterations observed. Knockdown of AK055347 inhibited cell viability of H9C2 cardiomyocytes, accompanied by downregulation of Cyp450 and ATP synthases. In addition, microarray analysis identified that MSS51 was a target of AK055347. Knockdown of AK055347 inhibited the expression of MSS51 in H9C2 cells. The observations of the current study suggest that lncRNAs are differentially expressed in the LA‑PV and LAA in patients with AF. AK055347 may contribute to the pathogenesis of AF by dysregulating mitochondrial energy production via regulation of Cyp450, ATP synthase and MSS51.
authors.affiliation: Department of Cardiology, The First Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang 150001, P.R. China.
authors.forename: Guiying
authors.initials: G
authors.lastname: Chen
authors.affiliation: Department of Cardiology, The First Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang 150001, P.R. China.
authors.forename: Hong
authors.initials: H
authors.lastname: Guo
authors.affiliation: Department of Cardiology, The First Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang 150001, P.R. China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Song
authors.affiliation: Department of Cardiology, The First Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang 150001, P.R. China.
authors.forename: Huiying
authors.initials: H
authors.lastname: Chang
authors.affiliation: Department of Cardiology, The First Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang 150001, P.R. China.
authors.forename: Shaojun
authors.initials: S
authors.lastname: Wang
authors.affiliation: Department of Cardiology, The First Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang 150001, P.R. China.
authors.forename: Miaomiao
authors.initials: M
authors.lastname: Zhang
authors.affiliation: Department of Cardiology, The First Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang 150001, P.R. China.
authors.forename: Chang
authors.initials: C
authors.lastname: Liu
mesh_terms: D000818:Animals; D001281:Atrial Fibrillation / Q000235:genetics* / Q000378:metabolism*; D002460:Cell Line; D002470:Cell Survival / Q000235:genetics; D016000:Cluster Analysis; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics; D004734:Energy Metabolism; D020869:Gene Expression Profiling; D055785:Gene Knockdown Techniques; D006801:Humans; D008928:Mitochondria / Q000378:metabolism*; D032383:Myocytes, Cardiac / Q000378:metabolism*; D062085:RNA, Long Noncoding / Q000235:genetics*; D034741:RNA, Small Interfering / Q000235:genetics; D051381:Rats
chemical_list: D062085:RNA, Long Noncoding; D034741:RNA, Small Interfering; D003577:Cytochrome P-450 Enzyme System