title: Pre-sowing Seed Treatment with 24-Epibrassinolide Ameliorates Pesticide Stress inBrassica junceaL. through the Modulation of Stress Markers.
abstract: The present experiment was designed to assess the effects of seed soaking with 24-epibrassinolide (EBR) on the physiology ofBrassica junceaL. seedlings grown under imidacloprid (IMI) toxicity. Application of EBR increased the length of seedlings, dry weight, and pigment contents, polyphenols, total phenols, and organic acids under IMI toxicity. The expression of genes coding key enzymes of pigment, phenols, polyphenols, and organic acid biosynthetic pathways was also studied includingCHLASE(chlorophyllase),PSY(phytoene synthase),CHS(chalcone synthase) andPAL(phenylalanine ammonialyase),CS(citrate synthase),SUCLG1(succinyl Co-A ligase,),SDH(succinate dehydrogenase),FH(fumarate hydratase),MS(malate synthase). Multiple linear regression (MLR) analysis revealed that IMI application regressed negatively on seedling length, dry weight and total chlorophyll content. However, EBR seed treatment regressed positively on all the parameters studied. Moreover, interaction between IMI and EBR showed positive regression for growth parameters, content of pigments, total polyphenol, total phenol and malate, and expression ofPSYandPAL. Negative interactions were noticed for the contents of fumarate, succinate and citrate, and expression ofCHSand all genes studied related to organic acid metabolism. In conclusion, EBR enhanced the growth and contents of all studied metabolites by regulating the gene expression ofB. junceaseedlings under IMI stress.
authors.affiliation: Plant Stress Physiology Lab, Department of Botanical and Environmental Sciences, Guru Nanak Dev University Amritsar, India.
authors.forename: Anket
authors.initials: A
authors.lastname: Sharma
authors.affiliation: Department of Molecular Biology and Biochemistry, Guru Nanak Dev University Amritsar, India.
authors.forename: Sharad
authors.initials: S
authors.lastname: Thakur
authors.affiliation: Plant Stress Physiology Lab, Department of Botanical and Environmental Sciences, Guru Nanak Dev University Amritsar, India.
authors.forename: Vinod
authors.initials: V
authors.lastname: Kumar
authors.affiliation: Department of Botany and Environmental Science, Sri Guru Granth Sahib World University Fatehgarh Sahib, India.
authors.forename: Mukesh K
authors.initials: MK
authors.lastname: Kanwar
authors.affiliation: Department of Molecular Biology and Biochemistry, Guru Nanak Dev University Amritsar, India.
authors.forename: Anup K
authors.initials: AK
authors.lastname: Kesavan
authors.affiliation: Plant Stress Physiology Lab, Department of Botanical and Environmental Sciences, Guru Nanak Dev University Amritsar, India.
authors.forename: Ashwani K
authors.initials: AK
authors.lastname: Thukral
authors.affiliation: Plant Stress Physiology Lab, Department of Botanical and Environmental Sciences, Guru Nanak Dev University Amritsar, India.
authors.forename: Renu
authors.initials: R
authors.lastname: Bhardwaj
authors.affiliation: Biology Department, College of Science and Humanities, Prince Sattam bin Abdulaziz University Alkharj, Saudi Arabia.
authors.forename: Pravej
authors.initials: P
authors.lastname: Alam
authors.affiliation: Department of Botany and Microbiology, Faculty of Science, King Saud UniversityRiyadh, Saudi Arabia; Department of Botany, S. P. CollegeSrinagar, India.
authors.forename: Parvaiz
authors.initials: P
authors.lastname: Ahmad
keywords: GC-MS; HPLC; brassinosteroids; mustard; phenylalanine ammonialyase; pigments; total phenols