title: Application of a dual target PCR-high resolution melting (HRM) method for rapid nontuberculous mycobacteria identification.
abstract: Species differentiation of nontuberculous mycobacteria (NTM) has long been a difficult task in clinical laboratories. This study demonstrated and evaluated a simple and cost-effective method using the real-time PCR with high-resolution melting (PCR-HRM) analysis technique, which could differentiate at least 14 different medically related NTM.
authors.affiliation: Department of Microbiology, Queen Mary Hospital, The University of Hong Kong, Hong Kong Special Administrative Region.
authors.forename: Jonathan Hk
authors.initials: JH
authors.lastname: Chen
authors.affiliation: Department of Microbiology, Queen Mary Hospital, The University of Hong Kong, Hong Kong Special Administrative Region; Carol Yu Centre for Infection, The University of Hong Kong, Hong Kong Special Administrative Region.
authors.forename: Vincent Cc
authors.initials: VC
authors.lastname: Cheng
authors.affiliation: Department of Microbiology, Queen Mary Hospital, The University of Hong Kong, Hong Kong Special Administrative Region.
authors.forename: Kevin Kk
authors.initials: KK
authors.lastname: She
authors.affiliation: Department of Microbiology, Queen Mary Hospital, The University of Hong Kong, Hong Kong Special Administrative Region.
authors.forename: Wing-Cheong
authors.initials: WC
authors.lastname: Yam
authors.affiliation: Department of Microbiology, Queen Mary Hospital, The University of Hong Kong, Hong Kong Special Administrative Region; Carol Yu Centre for Infection, The University of Hong Kong, Hong Kong Special Administrative Region. Electronic address: kyyuen@hkucc.hku.hk.
authors.forename: Kwok-Yung
authors.initials: KY
authors.lastname: Yuen
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics; D018834:Chaperonin 60 / Q000235:genetics; D003362:Cost-Benefit Analysis; D004269:DNA, Bacterial / Q000302:isolation & purification*; D005798:Genes, Bacterial; D009170:Nontuberculous Mycobacteria / Q000145:classification* / Q000302:isolation & purification*; D012336:RNA, Ribosomal, 16S / Q000302:isolation & purification; D060888:Real-Time Polymerase Chain Reaction
chemical_list: D001426:Bacterial Proteins; D018834:Chaperonin 60; D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S; C074119:heat-shock protein 65, Mycobacterium
keywords: High-resolution melting; Nontuberculous mycobacteria