title: Extracts containing CLPs of Bacillus amyloliquefaciens JN68 isolated from chicken intestines exert antimicrobial effects, particularly on methicillin-resistant Staphylococcus aureus and Listeria monocytogenes.
abstract: Bacillus amyloliquefaciens JN68, which has been discussed with regards to its antimicrobial activities, was successfully isolated from healthy chicken intestines in the present study. Using the spot-on-the-lawn antagonism method, the preliminary study indicated that a suspension culture of the B. amyloliquefaciens JN68 strain can inhibit the growth of Aspergillus niger and Penicillium pinophilum. Furthermore, the cyclic lipopeptides (CLPs) produced by the B. amyloliquefaciens JN68 strain were further purified through acid precipitation and Bond Elut®C18 chromatography, and their structures were identified using the liquid chromatography‑electrospray ionization‑mass spectrometry (MS)/MS method. Purified CLPs exerted broad spectrum antimicrobial activities on various pathogenic and foodborne bacteria and fungi, as determined using the agar well diffusion method. Listeria monocytogenes can induce listeriosis, which is associated with a high mortality rate. Methicillin‑resistant Staphylococcus aureus (MRSA) is a major pathogenic bacteria that causes nosocomial infections. Therefore, L. monocytogenes and MRSA are currently of great concern. The present study aimed to determine whether B. amyloliquefaciens JN68 extracts could inhibit L. monocytogenes and MRSA. The results indicated that extracts of B. amyloliquefaciens JN68 have CLP components, and can successfully inhibit the growth of L. monocytogenes and MRSA.
authors.affiliation: Department of Nutrition, Master Program of Biomedical Nutrition, Hungkuang University, Taichung 433, Taiwan, R.O.C.
authors.forename: Jen-Ni
authors.initials: JN
authors.lastname: Chen
authors.affiliation: Department of Nutrition, Master Program of Biomedical Nutrition, Hungkuang University, Taichung 433, Taiwan, R.O.C.
authors.forename: Chyou-Wei
authors.initials: CW
authors.lastname: Wei
authors.affiliation: Department of Nutrition, Master Program of Biomedical Nutrition, Hungkuang University, Taichung 433, Taiwan, R.O.C.
authors.forename: Hsiao-Chun
authors.initials: HC
authors.lastname: Liu
authors.affiliation: Department of Nursing, Hungkuang University, Taichung 433, Taiwan, R.O.C.
authors.forename: Shu-Ying
authors.initials: SY
authors.lastname: Chen
authors.affiliation: Department of Food Science and Biotechnology, National Chung Hsing University, Taichung 402, Taiwan, R.O.C.
authors.forename: Chinshuh
authors.initials: C
authors.lastname: Chen
authors.affiliation: Institute of Molecular Biology, National Chung Hsing University, Taichung 402, Taiwan, R.O.C.
authors.forename: Yu-Min
authors.initials: YM
authors.lastname: Juang
authors.affiliation: Institute of Molecular Biology, National Chung Hsing University, Taichung 402, Taiwan, R.O.C.
authors.forename: Chien-Chen
authors.initials: CC
authors.lastname: Lai
authors.affiliation: Department of Emergency Medicine, School of Medicine, Tzu Chi University, Hualien 970, Taiwan, R.O.C.
authors.forename: Giou-Teng
authors.initials: GT
authors.lastname: Yiang
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000302:isolation & purification / Q000494:pharmacology; D000890:Anti-Infective Agents / Q000302:isolation & purification / Q000494:pharmacology*; D000898:Antibiosis; D000069977:Bacillus amyloliquefaciens / Q000302:isolation & purification / Q000378:metabolism*; D002645:Chickens; D007422:Intestines / Q000382:microbiology; D055666:Lipopeptides / Q000302:isolation & purification / Q000494:pharmacology*; D008089:Listeria monocytogenes / Q000187:drug effects*; D055624:Methicillin-Resistant Staphylococcus aureus / Q000187:drug effects*; D010456:Peptides, Cyclic / Q000302:isolation & purification / Q000494:pharmacology*
chemical_list: D000900:Anti-Bacterial Agents; D000890:Anti-Infective Agents; D055666:Lipopeptides; D010456:Peptides, Cyclic