title: Cold-inducible RNA binding protein in mouse mammary gland development.
abstract: RNA binding proteins (RBPs) regulate gene expression by controlling mRNA export, translation, and stability. When altered, some RBPs allow cancer cells to grow, survive, and metastasize. Cold-inducible RNA binding protein (CIRP) is overexpressed in a subset of breast cancers, induces proliferation in breast cancer cell lines, and inhibits apoptosis. Although studies have begun to examine the role of CIRP in breast and other cancers, its role in normal breast development has not been assessed. We generated a transgenic mouse model overexpressing human CIRP in the mammary epithelium to ask if it plays a role in mammary gland development. Effects of CIRP overexpression on mammary gland morphology, cell proliferation, and apoptosis were studied from puberty through pregnancy, lactation and weaning. There were no gross effects on mammary gland morphology as shown by whole mounts. Immunohistochemistry for the proliferation marker Ki67 showed decreased proliferation during the lactational switch (the transition from pregnancy to lactation) in mammary glands from CIRP transgenic mice. Two markers of apoptosis showed that the transgene did not affect apoptosis during mammary gland involution. These results suggest a potential in vivo function in suppressing proliferation during a specific developmental transition.
authors.affiliation: Department of Cell Biology and Physiology, University of New Mexico School of Medicine and University of New Mexico Cancer Center, Albuquerque, NM, United States.
authors.forename: Daniel A
authors.initials: DA
authors.lastname: Lujan
authors.affiliation: Department of Cell Biology and Physiology, University of New Mexico School of Medicine and University of New Mexico Cancer Center, Albuquerque, NM, United States.
authors.forename: Selina
authors.initials: S
authors.lastname: Garcia
authors.affiliation: Department of Cell Biology and Physiology, University of New Mexico School of Medicine and University of New Mexico Cancer Center, Albuquerque, NM, United States.
authors.forename: Jennifer
authors.initials: J
authors.lastname: Vanderhoof
authors.affiliation: Department of Cell Biology and Physiology, University of New Mexico School of Medicine and University of New Mexico Cancer Center, Albuquerque, NM, United States.
authors.forename: Joshua
authors.initials: J
authors.lastname: Sifuentes
authors.affiliation: Department of Cell Biology and Physiology, University of New Mexico School of Medicine and University of New Mexico Cancer Center, Albuquerque, NM, United States.
authors.forename: Yekaterina
authors.initials: Y
authors.lastname: Brandt
authors.affiliation: Department of Cell Biology and Physiology, University of New Mexico School of Medicine and University of New Mexico Cancer Center, Albuquerque, NM, United States; Department of Medicine, University of Florida, Gainesville, FL, United States.
authors.forename: Yuehan
authors.initials: Y
authors.lastname: Wu
authors.affiliation: Department of Cell Biology and Physiology, University of New Mexico School of Medicine and University of New Mexico Cancer Center, Albuquerque, NM, United States.
authors.forename: Xun
authors.initials: X
authors.lastname: Guo
authors.affiliation: Department of Cell Biology and Physiology, University of New Mexico School of Medicine and University of New Mexico Cancer Center, Albuquerque, NM, United States.
authors.forename: Therese
authors.initials: T
authors.lastname: Mitchell
authors.affiliation: Department of Cell Biology and Physiology, University of New Mexico School of Medicine and University of New Mexico Cancer Center, Albuquerque, NM, United States.
authors.forename: Tamara
authors.initials: T
authors.lastname: Howard
authors.affiliation: Department of Cell Biology and Physiology, University of New Mexico School of Medicine and University of New Mexico Cancer Center, Albuquerque, NM, United States.
authors.forename: Helen J
authors.initials: HJ
authors.lastname: Hathaway
authors.affiliation: Department of Cell Biology and Physiology, University of New Mexico School of Medicine and University of New Mexico Cancer Center, Albuquerque, NM, United States. Electronic address: rhartley@salud.unm.edu.
authors.forename: Rebecca S
authors.initials: RS
authors.lastname: Hartley
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000235:genetics; D049109:Cell Proliferation / Q000235:genetics*; D005260:Female; D018507:Gene Expression Regulation, Developmental; D006801:Humans; D019394:Ki-67 Antigen / Q000096:biosynthesis; D007774:Lactation / Q000235:genetics; D008321:Mammary Glands, Animal / Q000254:growth & development / Q000378:metabolism*; D051379:Mice; D008822:Mice, Transgenic; D011247:Pregnancy; D016601:RNA-Binding Proteins / Q000096:biosynthesis* / Q000235:genetics; D014886:Weaning
chemical_list: C491390:CIRBP protein, human; D019394:Ki-67 Antigen; D016601:RNA-Binding Proteins
keywords: A18 hnRNP; CIRP; MMTV-LTR; Mammary gland development; RNA binding protein; Transgenic mouse