title: Probing mucin interaction behavior of magnetic nanoparticles.
abstract: In this study, we developed iron oxide based magnetic nanoparticles (MNPs) by precipitation of iron salts in the presence of ammonia and created four different formulations: without functionality (plain MNPs, no coating), with β-cyclodextrin (MNPs+β-CD) or pluronic 127 polymer (MNPs+F-127), and both β-cyclodextrin and pluronic 127 polymer (MNPs+β-CD-F-127) functionality for its efficient use in mucosal delivery. We studied the interaction and/or binding behavior of these MNPs formulations with porcine stomach mucin using steady-state fluorescence spectroscopy, and then quantified the bound mucin from absorption studies. Toxicity of these MNPs against cervical cancer cells and red blood cells was evaluated. Ex-vivo studies were performed using freshly collected gastrointestinal, ovarian, pancreas and colon organ tissues of pig to evaluate binding and uptake phenomenon of MNPs. Transport studies of these MNPs in mucin was evaluated using Boyden's chamber assay. All these studies together suggest that the MNPs+β-CD-F-127 formulation was strongly interacted with mucin and interestingly transported through mucin compared to other MNPs formulations. Hence, MNPs+β-CD-F-127 formulation could be a good candidate for the mucoadhesive biopharmaceuticals and drug delivery system.
authors.affiliation: Department of Pharmaceutical Sciences and Center for Cancer Research, University of Tennessee Health Science Center, Memphis, TN 38105, USA; Department of Materials Science and Nanotechnology, Yogi Vemana University, Kadapa 516 001, AP, India.
authors.forename: Vijayakumar N
authors.initials: VN
authors.lastname: Boya
authors.affiliation: Department of Pharmaceutical Sciences and Center for Cancer Research, University of Tennessee Health Science Center, Memphis, TN 38105, USA.
authors.forename: Renn
authors.initials: R
authors.lastname: Lovett
authors.affiliation: Department of Pharmaceutical Sciences and Center for Cancer Research, University of Tennessee Health Science Center, Memphis, TN 38105, USA.
authors.forename: Saini
authors.initials: S
authors.lastname: Setua
authors.affiliation: Department of Pharmaceutical Sciences and Center for Cancer Research, University of Tennessee Health Science Center, Memphis, TN 38105, USA.
authors.forename: Vaibhav
authors.initials: V
authors.lastname: Gandhi
authors.affiliation: Department of Pharmaceutical Sciences and Center for Cancer Research, University of Tennessee Health Science Center, Memphis, TN 38105, USA.
authors.forename: Prashanth K B
authors.initials: PKB
authors.lastname: Nagesh
authors.affiliation: Department of Pharmaceutical Sciences and Center for Cancer Research, University of Tennessee Health Science Center, Memphis, TN 38105, USA.
authors.forename: Sheema
authors.initials: S
authors.lastname: Khan
authors.affiliation: Department of Pharmaceutical Sciences and Center for Cancer Research, University of Tennessee Health Science Center, Memphis, TN 38105, USA.
authors.forename: Meena
authors.initials: M
authors.lastname: Jaggi
authors.affiliation: Department of Pharmaceutical Sciences and Center for Cancer Research, University of Tennessee Health Science Center, Memphis, TN 38105, USA. Electronic address: myallapu@uthsc.edu.
authors.forename: Murali M
authors.initials: MM
authors.lastname: Yallapu
authors.affiliation: Department of Pharmaceutical Sciences and Center for Cancer Research, University of Tennessee Health Science Center, Memphis, TN 38105, USA. Electronic address: schauhan1@uthsc.edu.
authors.forename: Subhash C
authors.initials: SC
authors.lastname: Chauhan
mesh_terms: D000641:Ammonia / Q000737:chemistry; D000818:Animals; D001692:Biological Transport; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D003106:Colon / Q000378:metabolism; D004337:Drug Carriers / Q000138:chemical synthesis / Q000378:metabolism* / Q000493:pharmacokinetics; D005260:Female; D005290:Ferric Compounds / Q000737:chemistry; D005753:Gastric Mucosa / Q000378:metabolism; D006801:Humans; D058185:Magnetite Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D009077:Mucins / Q000378:metabolism*; D010053:Ovary / Q000378:metabolism; D010179:Pancreas / Q000378:metabolism; D020442:Poloxamer / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D013552:Swine; D047392:beta-Cyclodextrins / Q000737:chemistry*
chemical_list: D004337:Drug Carriers; D005290:Ferric Compounds; D058185:Magnetite Nanoparticles; D009077:Mucins; D047392:beta-Cyclodextrins; D020442:Poloxamer; C000499:ferric oxide; D000641:Ammonia; C031215:betadex
keywords: Cancer; Drug delivery; Magnetic nanoparticles; Mucin; Mucoadhesive pharmaceuticals