title: Decreased plasma miR-335 expression in patients with acute ischemic stroke and its association with calmodulin expression.
abstract: Objective To determine the expression and clinical significance of plasma miR-335 in patients with acute ischemic stroke (AIS) and investigate its association with calmodulin (CaM) expression. Methods Plasma miR-335 and CaM expression levels in patients with AIS and healthy controls were examined. Correlations between miR-335, CaM, and National Institutes of Health Stroke Scale scores were also analysed. Furthermore, the potential regulatory function of miR-335 on CaM expression was investigated. Results Plasma miR-335 levels were significantly lower in AIS and negatively correlated with NIHSS scores. The converse was observed for plasma CaM levels. Plasma miR-335 and CaM levels were negatively correlated. Plasma miR-335 was confirmed as a novel biomarker for AIS diagnosis and an independent predictor of AIS. Up-regulation of miR-335 suppressed CaM protein expression, and CaM was confirmed as a direct target of miR-335. Conclusions Plasma miR-335 was down-regulated in AIS patients and represents a potential noninvasive circulating biomarker.
authors.affiliation: 1 Department of Neurology, Tianjin Medical University General Hospital, Tianjin, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Zhao
authors.affiliation: 2 Department of Neurology, Tianjin Huanhu Hospital, Tianjin, China.
authors.forename: Zilong
authors.initials: Z
authors.lastname: Zhu
authors.affiliation: 1 Department of Neurology, Tianjin Medical University General Hospital, Tianjin, China.
authors.forename: Junwei
authors.initials: J
authors.lastname: Hao
authors.affiliation: 3 Department of Pharmacology, Logistics College of Chinese People's Armed Police Forces, Tianjin, China.
authors.forename: Zongming
authors.initials: Z
authors.lastname: Wan
authors.affiliation: 4 Department of Pathogenic Biology and Immunology, Logistics College of Chinese People's Armed Police Forces, Tianjin, China.
authors.forename: Xiaoqin
authors.initials: X
authors.lastname: Guo
mesh_terms: D000368:Aged; D001483:Base Sequence; D001665:Binding Sites; D015415:Biomarkers / Q000097:blood; D002545:Brain Ischemia / Q000097:blood / Q000175:diagnosis / Q000235:genetics* / Q000473:pathology; D002147:Calmodulin / Q000097:blood / Q000235:genetics*; D016022:Case-Control Studies; D005260:Female; D005786:Gene Expression Regulation; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000097:blood / Q000235:genetics*; D008875:Middle Aged; D015398:Signal Transduction; D020521:Stroke / Q000097:blood / Q000175:diagnosis / Q000235:genetics* / Q000473:pathology
chemical_list: D015415:Biomarkers; D002147:Calmodulin; C556347:MIRN335 microRNA, human; D035683:MicroRNAs
keywords: MicroRNA-335; acute ischemic stroke; calmodulin; diagnosis