title: Humoral immune response and coated or uncoated oxygenators during cardiopulmonary bypass surgery.
abstract: OBJECTIVE
  To investigate and compare uncoated and phosphorylcholine-coated oxygenators in terms of induction of humoral immune response during coronary artery bypass surgery.
  
  
  METHODS
  A total of 20 consecutive patients who underwent coronary artery bypass surgery were randomly distributed into two groups according to the type of oxygenator used during surgery. Group 1 consisted of 10 patients who were operated on using phosphorylcholine-coated oxygenators. Group 2 contained 10 patients who underwent surgery using uncoated oxygenators. Blood and oxygenator fibre samples were obtained and compared in terms of immunoglobulins (IgG, IgM), complements (C3c, C4), serum total protein and albumin levels using electron microscopy and flow cytometry.
  
  
  RESULTS
  In group 1, levels of IgM, IgG, total protein and serum albumin were significantly increased at the end of cardiopulmonary bypass (CPB) compared to those at the beginning of CPB. In group 2, C3c and C4 levels at the beginning of CPB were found to be significantly higher than at the end. Electron microscopic examination of oxygenator fibres demonstrated that phosphorylcholine-coated fibres were less likely to be adsorbed by serum proteins and complements than the uncoated fibres.
  
  
  CONCLUSIONS
  Our results indicate that phosphorylcholine-coated oxygenators seemed to induce humoral immune response to a lesser extent than uncoated oxygenators during coronary artery bypass procedures.
authors.affiliation: Department of Cardiovascular Surgery, Faculty of Medicine, Recep Tayyip Erdogan University, Training and Research Hospital, Rize, Turkey.
authors.forename: Sedat Ozan
authors.initials: SO
authors.lastname: Karakisi
authors.affiliation: Department of Cardiovascular Surgery, Faculty of Medicine, Yıldırım Beyazıd University, Atatürk Training and Research Hospital, Ankara, Turkey.
authors.forename: Ayşe Gül
authors.initials: AG
authors.lastname: Kunt
authors.affiliation: Department of Cardiovascular Surgery, Faculty of Medicine, Recep Tayyip Erdogan University, Training and Research Hospital, Rize, Turkey.
authors.forename: Şahin
authors.initials: Ş
authors.lastname: Bozok
authors.affiliation: Department of Biomedical Engineering, Baskent University, Ankara, Turkey.
authors.forename: İdil
authors.initials: İ
authors.lastname: Çankaya
authors.affiliation: Department of Biomedical Engineering, Baskent University, Ankara, Turkey.
authors.forename: Mustafa
authors.initials: M
authors.lastname: Kocakulak
authors.affiliation: Department of Immunology, Gulhane Military Medical Academy and School of Medicine, Ankara, Turkey.
authors.forename: Uğur
authors.initials: U
authors.lastname: Muşabak
authors.affiliation: Department of Anatomy, Faculty of Medicine, Hacettepe University, Ankara, Turkey.
authors.forename: Mustafa Fevzi
authors.initials: MF
authors.lastname: Sargon
authors.affiliation: Department of Cardiovascular Surgery, Faculty of Medicine, Recep Tayyip Erdogan University, Training and Research Hospital, Rize, Turkey.
authors.forename: Şaban
authors.initials: Ş
authors.lastname: Ergene
authors.affiliation: Department of Cardiovascular Surgery, Faculty of Medicine, Recep Tayyip Erdogan University, Training and Research Hospital, Rize, Turkey.
authors.forename: Gökhan
authors.initials: G
authors.lastname: İlhan
authors.affiliation: Department of Cardiovascular Surgery, Faculty of Medicine, Recep Tayyip Erdogan University, Training and Research Hospital, Rize, Turkey.
authors.forename: Hakan
authors.initials: H
authors.lastname: Karamustafa
authors.affiliation: Department of Cardiovascular Surgery, Faculty of Medicine, Recep Tayyip Erdogan University, Training and Research Hospital, Rize, Turkey.
authors.forename: Nebiye
authors.initials: N
authors.lastname: Tufekci
authors.affiliation: Department of Cardiovascular Surgery, Faculty of Medicine, Yıldırım Beyazıd University, Atatürk Training and Research Hospital, Ankara, Turkey.
authors.forename: Erol
authors.initials: E
authors.lastname: Şener
mesh_terms: D000327:Adsorption; D000368:Aged; D015415:Biomarkers / Q000097:blood; D002315:Cardiopulmonary Bypass / Q000009:adverse effects / Q000295:instrumentation*; D020099:Coated Materials, Biocompatible / Q000009:adverse effects; D015932:Complement C3c / Q000378:metabolism; D003181:Complement C4 / Q000378:metabolism; D001026:Coronary Artery Bypass / Q000009:adverse effects; D003430:Cross-Sectional Studies; D004867:Equipment Design; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D056724:Immunity, Humoral; D007074:Immunoglobulin G / Q000097:blood; D007075:Immunoglobulin M / Q000097:blood; D008297:Male; D008854:Microscopy, Electron; D008875:Middle Aged; D010107:Oxygenators, Membrane / Q000009:adverse effects; D010767:Phosphorylcholine / Q000009:adverse effects / Q000276:immunology* / Q000378:metabolism; D012709:Serum Albumin / Q000378:metabolism; D000075462:Serum Albumin, Human; D013499:Surface Properties; D016896:Treatment Outcome; D014421:Turkey
chemical_list: C422455:ALB protein, human; D015415:Biomarkers; D020099:Coated Materials, Biocompatible; D003181:Complement C4; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D012709:Serum Albumin; D010767:Phosphorylcholine; D015932:Complement C3c; D000075462:Serum Albumin, Human