title: Determination of Relative Response Factors of Cefazolin Impurities by Quantitative NMR.
abstract: The relative response factors (RRFs) of ten cefazolin impurities were determined by quantitative nuclear magnetic resonance (qNMR) and high-performance liquid chromatography (HPLC) equipped with an ultraviolet (UV) detector. The purities of these ten cefazolin impurities were successfully measured by qNMR for the purpose of RRFs determination by HPLC. The RRF values and their uncertainties determined by the two approaches are comparable. While the qNMR approach is effective and makes it easier to determine the RRFs for impurities, it also has the advantage of allowing the universal detection of protons without the limitations of common mass detectors. The use of qNMR provides a reliable and universal method for the RRF determination of impurities.
authors.affiliation: College of Chemistry and Chemical Engineering, Shanghai University of Engineering Science, Shanghai, 201620, China.
authors.forename: Shuyu
authors.initials: S
authors.lastname: Liu
authors.affiliation: National Institutes for Food and Drug Control, No.2 Tiantan Xili, Dongcheng, Beijing, 100050, China.
authors.forename: Shangchen
authors.initials: S
authors.lastname: Yao
authors.affiliation: College of Chemistry and Chemical Engineering, Shanghai University of Engineering Science, Shanghai, 201620, China.
authors.forename: Hailing
authors.initials: H
authors.lastname: Zhang
authors.affiliation: National Institutes for Food and Drug Control, No.2 Tiantan Xili, Dongcheng, Beijing, 100050, China. hucq@nicpbp.org.cn.
authors.forename: Changqin
authors.initials: C
authors.lastname: Hu
mesh_terms: D000900:Anti-Bacterial Agents / Q000032:analysis / Q000737:chemistry; D002437:Cefazolin / Q000032:analysis* / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004340:Drug Contamination; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D011522:Protons
chemical_list: D000900:Anti-Bacterial Agents; D011522:Protons; D002437:Cefazolin
keywords: HPLC; RRF; qNMR; uncertainty