title: Proteomic Analysis of a Rat Cerebral Ischemic Injury Model after Human Cerebral Endothelial Cell Transplantation.
abstract: OBJECTIVE
  Cerebral endothelial cells have unique biological features and are fascinating candidate cells for stroke therapy.
  
  
  METHODS
  In order to understand the molecular mechanisms of human cerebral endothelial cell (hCMEC/D3) transplantation in a rat stroke model, we performed proteomic analysis using 2-dimensional electrophoresis and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Protein expression was confirmed by quantitative real-time PCR and Western blot.
  
  
  RESULTS
  Several protein spots were identified by gel electrophoresis in the sham, cerebral ischemia (CI), and CI with hCMEC/D3 treatment cerebral ischemia with cell transplantation (CT) groups, and we identified 14 differentially expressed proteins in the CT group. Proteins involved in mitochondrial dysfunction (paraplegin matrix AAA peptidase subunit, SPG7), neuroinflammation (peroxiredoxin 6, PRDX6), and neuronal death (zinc finger protein 90, ZFP90) were markedly reduced in the CT group compared with the CI group. The expression of chloride intracellular channel 4 proteins involved in post-ischemic vasculogenesis was significantly decreased in the CI group but comparable to sham in the CT group.
  
  
  CONCLUSIONS
  These results contribute to our understanding of the early phase processes that follow cerebral endothelial cell treatment in CI. Moreover, some of the identified proteins may present promising new targets for stroke therapy.
authors.affiliation: Department of Neurosurgery, Gwangju Christian Hospital, Gwangju, Korea.; Department of Forensic Medicine, Chonnam National University Medical School, Gwangju, Korea.
authors.forename: Tae-Min
authors.initials: TM
authors.lastname: Choi
authors.affiliation: Department of Nuclear Medicine, Chonnam National University Medical School, Gwangju, Korea.
authors.forename: Misun
authors.initials: M
authors.lastname: Yun
authors.affiliation: Department of Neurology, Chonnam National University Medical School, Gwangju, Korea.
authors.forename: Jung-Kil
authors.initials: JK
authors.lastname: Lee
authors.affiliation: Department of Forensic Medicine, Chonnam National University Medical School, Gwangju, Korea.
authors.forename: Jong-Tae
authors.initials: JT
authors.lastname: Park
authors.affiliation: Department of Neurosurgery, Chonnam National University Medical School, Gwangju, Korea.
authors.forename: Man-Seok
authors.initials: MS
authors.lastname: Park
authors.affiliation: Department of Forensic Medicine, Chonnam National University Medical School, Gwangju, Korea.; Center for Creative Biomedical Scientists, Chonnam National University Medical School, Gwangju, Korea.
authors.forename: Hyung-Seok
authors.initials: HS
authors.lastname: Kim
keywords: 2-dimensional electrophoresis; Brain; Cell therapy; Ischemia; Proteomics