title: Cadmium Telluride Quantum Dots as a Fluorescence Marker for Adipose Tissue Grafts.
abstract: Plastic and reconstructive surgeons increasingly apply adipose tissue grafting in a clinical setting, although the anticipation of graft survival is insecure. There are only few tools for tracking transplanted fat grafts in vivo.Murine adipose tissue clusters were incubated with negatively charged, mercaptoproprionic acid-coated cadmium telluride quantum dots (QDs) emitting in the dark red or near infrared. The intracellular localization of QDs was studied by confocal laser scanning microscopy.As a result, the adipose tissue clusters showed a proportional increase in fluorescence with increasing concentrations (1, 10, 16, 30, 50 nM) of cadmium telluride QDs. Laser scanning microscopy demonstrated a membrane bound localization of QDs. Vacuoles and cell nuclei of adipocytes were spared by QDs. We conclude that QDs were for the first time proven intracellular in adult adipocytes and demonstrate a strong fluorescence signal. Therefore, they may play an essential role for in vivo tracking of fat grafts.
authors.affiliation: From the *Hand, Plastic and Aesthetic Surgery, Surgical Department, Klinikum Großhadern, Ludwig Maximilians University; †Centre for Hand Surgery, Microsurgery and Plastic Surgery, Schoen-Klinik Muenchen Harlaching; ‡Centre for Functional Photonics (CFP), Department of Physics and Material Science, City University of Hong Kong, Hong Kong; §Center for Nanoscience (CeNS), ∥Center for System Based Drug Research, Department of Pharmacy, Pharmaceutical Biotechnology, Ludwig Maximilians University, Munich, Germany; and ¶MMCT Laboratory of Macromolecular Cancer Therapeutics, Department for Pharmaceutical Chemistry, Faculty Center for Pharmacy, University of Vienna, Wien, Austria.
authors.forename: Claus J
authors.initials: CJ
authors.lastname: Deglmann
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Błażków-Schmalzbauer
authors.forename: Sarah
authors.initials: S
authors.lastname: Moorkamp
authors.forename: Andrei S
authors.initials: AS
authors.lastname: Susha
authors.forename: Tanja
authors.initials: T
authors.lastname: Herrler
authors.forename: Riccardo E
authors.initials: RE
authors.lastname: Giunta
authors.forename: Ernst
authors.initials: E
authors.lastname: Wagner
authors.forename: Andrey L
authors.initials: AL
authors.lastname: Rogach
authors.forename: Ruediger G
authors.initials: RG
authors.lastname: Baumeister
authors.forename: Manfred
authors.initials: M
authors.lastname: Ogris
mesh_terms: D000818:Animals; D019187:Cadmium Compounds / Q000008:administration & dosage / Q000493:pharmacokinetics; D049408:Luminescent Agents / Q000008:administration & dosage / Q000493:pharmacokinetics; D051379:Mice; D008807:Mice, Inbred BALB C; D018613:Microscopy, Confocal; D045663:Quantum Dots / Q000008:administration & dosage; D019265:Spectroscopy, Near-Infrared; D050151:Subcutaneous Fat / Q000000981:diagnostic imaging* / Q000378:metabolism / Q000637:transplantation*; D013691:Tellurium / Q000008:administration & dosage / Q000493:pharmacokinetics
chemical_list: D019187:Cadmium Compounds; D049408:Luminescent Agents; D013691:Tellurium; C028337:cadmium telluride