title: Composition of The Essential Oil From Danggui-zhiqiao Herb-Pair and Its Analgesic Activity and Effect on Hemorheology in Rats With Blood Stasis Syndrome.
abstract: BACKGROUND
  Angelica sinensisandAurantii fructuused in a pair, named Danggui-Zhiqiao herb-pair (DZHP), which was rich in essential oil and has been adopted to promote blood circulation, dispel blood stasis, and relieve pain in traditional Chinese medicine (TCM).
  
  
  OBJECTIVE
  To analyze the composition and pharmacological effects of essential oil from DZHP.
  
  
  METHODS
  The composition of the essential oil from DZHP was analyzed by gas chromatography/mass spectrometry (GC/MS). Its analgesic activity was evaluated by acetic acid-induced writhing test and hot plate test. The hemorheology test was carried out to evaluate the effect on hemorheology in rats with blood stasis syndrome.
  
  
  RESULTS
  Twenty-eight components were identified and the main components wereα-pinene (3.07%),β-pinene (2.0%),β-myrcene (3.71%), D-limonene (49.28%),γ-terpinen (9.53%),α-terpinolene (1.80%),α-terpineol (2.02%),β-bisabolene (1.13%), butylidenephthalide (1.43%), and Z-ligustilide (16.08%). The pharmacology test showed that the essential oil significantly inhibited the number of writhes induced by acetic acid with inhibition rate of 44.64% and significantly increased hot-plate latency compared with control group from 30 to 90 min after oral administration of drugs in mice. It could significantly decrease plasma viscosity, whole blood relative index at high and low shear rate, whole blood reduced viscosity at high and low shear rate, and erythrocyte rigidity index in hemorheology test.
  
  
  CONCLUSIONS
  The composition of the essential oil of DZHP was determined successfully and it had analgesic and promoting blood circulation activities.
  
  
  CONCLUSIONS
  Angelica sinensisandAurantii fructuused in a pair, named Danggui-Zhiqiao herb-pair (DZHP), which was rich in Essential oil and has been adopted to promote blood circulation, dispel blood stasis and relieve pain in traditional Chinese medicine (TCM).Twenty-eight components were identified and the main components wereα-pinene (3.07%),β-pinene (2.0%),β-myrcene (3.71%), D-limonene (49.28%),γ-terpinen (9.53%),α-terpinolene (1.80%),α-terpineol (2.02%),β-bisabolene (1.13%), butylidenephthalide (1.43%), and Z-ligustilide (16.08%).The essential oil significantly inhibited the number of writhes induced by acetic acid and increased hot-plate latency compared with control group from 30 min to 90 min after oral administration of drugs in mice.The essential oil could significantly decrease plasma viscosity (PV), whole blood relative index (WBRI) at high and low shear rate, whole blood reduced viscosity (WBRV) at high and low shear rate, and erythrocyte rigidity index (ERI) in hemorheology test.Abbreviations used:DZHP: Danggui-Zhiqiao herb-pair; TCM: traditional Chinese medicine; GC/MS: gas chromatography /mass spectrometry; PV: plasma viscosity; WBRI: whole blood relative index; WBRV: whole blood reduced viscosity; ERI: erythrocyte rigidity index.
authors.affiliation: Department of biotechnology, Lab of Biotechnology and Engineering, School of Life Science and Technology, Central South University of Forestry and Technology, Changsha, China.
authors.forename: Yuanqing
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Chemistry, Hunan Province Engineering Research Center of Bioactive Substance Discovery of Traditional Chinese Medicine, Changsha, China.
authors.forename: Jianye
authors.initials: J
authors.lastname: Yan
authors.affiliation: Department of Chemistry, Hunan Province Engineering Research Center of Bioactive Substance Discovery of Traditional Chinese Medicine, Changsha, China.
authors.forename: Shunxiang
authors.initials: S
authors.lastname: Li
authors.affiliation: Department of Chemistry, TCM and Ethnomedicine Innovation and Development Laboratory, School of Pharmacy, Changsha, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.affiliation: Department of diagnosis, Diagnostic Institute of Traditional Chinese Medicine, Changsha, China.
authors.forename: Xiong
authors.initials: X
authors.lastname: Cai
authors.affiliation: Department of Chemistry, Hunan Province Engineering Research Center of Bioactive Substance Discovery of Traditional Chinese Medicine, Changsha, China; Department of Chemistry, Province & State Key Laboratory Breeding Base of Chinese medicine powder and Medicine Innovation in Hunan, Hunan University of Chinese Medicine, Changsha, China.
authors.forename: Dan
authors.initials: D
authors.lastname: Huang
authors.affiliation: Department of Chemistry, Hunan Province Engineering Research Center of Bioactive Substance Discovery of Traditional Chinese Medicine, Changsha, China.
authors.forename: Limin
authors.initials: L
authors.lastname: Gong
authors.affiliation: Department of diagnosis, Diagnostic Institute of Traditional Chinese Medicine, Changsha, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Li
keywords: Danggui-Zhiqiao herb-pair; analgesic activity; composition; essential oil; hemorheology