title: Luminescent Ruthenium(II) Polypyridyl Complexes as Nonviral Carriers for DNA Delivery.
abstract: Two new luminescent ruthenium(II) polypyridyl complexes, [Ru(bpy)2(tpt-phen)]Cl2(1; bpy=2,2'-bipyridine, tpt-phen=triptycenyl-1,10-phenanthroline) and [Ru(phen)2(tpt-phen)]Cl2(2; phen=1,10-phenanthroline), have been developed as potential nonviral vectors for DNA delivery. Photophysical and electrochemical properties of the complexes have been investigated and corroborated with electronic structure calculations. DNA condensation by these complexes has been investigated by UV/Vis and emission spectroscopy, circular dichroism spectroscopy, atomic force microscopy, dynamic light scattering, confocal microscopy, and electrophoretic mobility studies. These complexes interact with DNA and efficiently condense DNA into globular nanoparticles that are taken up efficiently by HeLa cells. DNA cleavage inability and biocompatibility of complexes have been explored. Both complexes have good gene transfection abilities.
authors.affiliation: Department of Chemistry, Karnatak University, Dharwad, 580003, Karnataka, India.
authors.forename: Satish S
authors.identifier: http://orcid.org/0000-0002-3250-9966
authors.initials: SS
authors.lastname: Bhat
authors.affiliation: Department of Chemistry, Karnatak University, Dharwad, 580003, Karnataka, India.
authors.forename: Vidyanand K
authors.initials: VK
authors.lastname: Revankar
authors.affiliation: Department of Chemistry, Savitribhai Phule University of Pune, Pune, 411007, Maharashtra, India.
authors.forename: Ayesha
authors.initials: A
authors.lastname: Khan
authors.affiliation: School of Chemical Science, Swami Ramanand Teerth Marathwada University, Nanded, 431606, Maharashtra, India.
authors.forename: Rahul V
authors.initials: RV
authors.lastname: Pinjari
authors.affiliation: Department of Chemistry, Masaryk University, Kotlarska 2, 61137, Brno, Czech Republic.|Central European Institute of Technology (CEITEC), Masaryk University, Kamenice 5, 62500, Brno, Czech Republic.
authors.forename: Marek
authors.initials: M
authors.lastname: Necas
mesh_terms: D002470:Cell Survival; D002942:Circular Dichroism; D056831:Coordination Complexes / Q000138:chemical synthesis / Q000737:chemistry*; D018360:Crystallography, X-Ray; D004247:DNA / Q000008:administration & dosage* / Q000737:chemistry; D018014:Gene Transfer Techniques / Q000295:instrumentation*; D005822:Genetic Vectors / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D049449:Luminescence; D018625:Microscopy, Atomic Force; D015394:Molecular Structure; D011725:Pyridines / Q000737:chemistry*; D011789:Quantum Theory; D012428:Ruthenium / Q000737:chemistry*
chemical_list: D056831:Coordination Complexes; D011725:Pyridines; D012428:Ruthenium; D004247:DNA
keywords: DNA; electrochemistry; luminescence; photochemistry; ruthenium