title: The Stress-Responsive and Host-Oriented Role of Nonribosomal Peptide Synthetases in an Entomopathogenic Fungus,Beauveria bassiana.
abstract: Beauveria bassianainfects a number of pest species and is known to produce insecticidal substances, such as the nonribosomal peptides (NRPs) beauvericin and bassianolide. However, most NRPs and their biological roles inB. bassianaremain undiscovered. To identify NRPs that potentially contribute to pathogenesis, the 21 predicted NRP synthetases (NRPSs) or NRPS-like proteins ofB. bassianaARSEF 2860 were primarily ranked into three functional groups: basic metabolism (7 NRPSs), pathogenicity (12 NRPSs), and unknown function (2 NRPSs). Based on the transcript levels during in vivo growth on diamondback moth (Plutella xylostella(Linnaeus)), half of the Group II NRPSs were likely to be involved in infection. Given that the metabolites biosynthesized by these NRPSs remain to be determined, our result underlines the importance of the NRPSome in fungal pathogenesis, and will serve as a guide for future genomic mining projects to discover functionally essential and structurally diverse NRPs in fungal genomes.
authors.affiliation: Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R. China.
authors.forename: Hang
authors.initials: H
authors.lastname: Liu
authors.affiliation: Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R. China.
authors.forename: Linan
authors.initials: L
authors.lastname: Xie
authors.affiliation: Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R. China.
authors.forename: Jing
authors.initials: J
authors.lastname: Wang
authors.affiliation: Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R. China.
authors.forename: Qiannan
authors.initials: Q
authors.lastname: Guo
authors.affiliation: Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R. China.
authors.forename: Shengnan
authors.initials: S
authors.lastname: Yang
authors.affiliation: Department of Entomology, China Agricultural University, Beijing 100083, P.R. China.
authors.forename: Pei
authors.initials: P
authors.lastname: Liang
authors.affiliation: Key Laboratory of Insect Developmental and Evolutionary Biology, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200032, P.R. China.
authors.forename: Chengshu
authors.initials: C
authors.lastname: Wang
authors.affiliation: Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R. China.
authors.forename: Min
authors.initials: M
authors.lastname: Lin
authors.affiliation: Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R. China.
authors.forename: Yuquan
authors.initials: Y
authors.lastname: Xu
authors.affiliation: Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R. China.
authors.forename: Liwen
authors.initials: L
authors.lastname: Zhang
mesh_terms: D001481:Basal Metabolism; D052982:Beauveria / Q000145:classification / Q000502:physiology*; D019143:Evolution, Molecular; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism; D020869:Gene Expression Profiling; D015966:Gene Expression Regulation, Fungal; D023061:Gene Order; D016681:Genome, Fungal; D054884:Host-Pathogen Interactions; D010453:Peptide Synthases / Q000235:genetics / Q000378:metabolism*; D010802:Phylogeny; D064210:Secondary Metabolism; D013312:Stress, Physiological
chemical_list: D005656:Fungal Proteins; D010453:Peptide Synthases; C104385:non-ribosomal peptide synthase
keywords: Nonribosomal peptide synthetase; entomopathogen; secondary metabolites