title: Gelatin/nanoceria nanocomposite fibers as antioxidant scaffolds for neuronal regeneration.
abstract: The design of efficient nerve conduits able to sustain the axonal outgrowth and its guidance towards appropriate targets is of paramount importance in nerve tissue engineering.
  
  
  
  In this work, we propose the preparation of highly aligned nanocomposite fibers of gelatin/cerium oxide nanoparticles (nanoceria), prepared by electrospinning. Nanoceria are powerful self-regenerative antioxidant nanomaterials, that behave as strong reactive oxygen species scavengers, and among various beneficial effects, they have been proven to inhibit the cell senescence and to promote the neurite sprouting.
  
  
  
  After a detailed characterization of the developed substrates, they have been tested on neuron-like SH-SY5Y cells, demonstrating strong antioxidant properties and beneficial multi-cue effects in terms of neurite development and alignment.
  
  
  
  Obtained findings suggest efficiency of the proposed substrates in providing combined topographical stimuli and antioxidant effects to cultured cells.
  
  
  
  Proposed nanocomposite scaffolds represent a promising approach for nerve tissue engineering and regenerative medicine.
authors.affiliation: Center for Micro-BioRobotics @SSSA, Istituto Italiano di Tecnologia, Viale Rinaldo Piaggio 34, 56025 Pontedera, Italy. Electronic address: attilio.marino@iit.it.
authors.forename: Attilio
authors.initials: A
authors.lastname: Marino
authors.affiliation: Nanobiotechnology Department, Istituto Italiano di Tecnologia, Via Morego 30, 16163 Genova, Italy.
authors.forename: Chiara
authors.initials: C
authors.lastname: Tonda-Turo
authors.affiliation: Center for Micro-BioRobotics @SSSA, Istituto Italiano di Tecnologia, Viale Rinaldo Piaggio 34, 56025 Pontedera, Italy.
authors.forename: Daniele
authors.initials: D
authors.lastname: De Pasquale
authors.affiliation: Department of Mechanical and Aerospace Engineering, Politecnico di Torino, Corso Duca degli Abruzzi 24, 10129 Torino, Italy.
authors.forename: Francesca
authors.initials: F
authors.lastname: Ruini
authors.affiliation: Center for Micro-BioRobotics @SSSA, Istituto Italiano di Tecnologia, Viale Rinaldo Piaggio 34, 56025 Pontedera, Italy.
authors.forename: Giada
authors.initials: G
authors.lastname: Genchi
authors.affiliation: Nanobiotechnology Department, Istituto Italiano di Tecnologia, Via Morego 30, 16163 Genova, Italy.
authors.forename: Simone
authors.initials: S
authors.lastname: Nitti
authors.affiliation: Center for Nanotechnology Innovation @NEST, Istituto Italiano di Tecnologia, Piazza San Silvestro 12, 56127 Pisa, Italy.
authors.forename: Valentina
authors.initials: V
authors.lastname: Cappello
authors.affiliation: Center for Nanotechnology Innovation @NEST, Istituto Italiano di Tecnologia, Piazza San Silvestro 12, 56127 Pisa, Italy.
authors.forename: Mauro
authors.initials: M
authors.lastname: Gemmi
authors.affiliation: Center for Micro-BioRobotics @SSSA, Istituto Italiano di Tecnologia, Viale Rinaldo Piaggio 34, 56025 Pontedera, Italy.
authors.forename: Virgilio
authors.initials: V
authors.lastname: Mattoli
authors.affiliation: Department of Mechanical and Aerospace Engineering, Politecnico di Torino, Corso Duca degli Abruzzi 24, 10129 Torino, Italy.
authors.forename: Gianluca
authors.initials: G
authors.lastname: Ciardelli
authors.affiliation: Center for Micro-BioRobotics @SSSA, Istituto Italiano di Tecnologia, Viale Rinaldo Piaggio 34, 56025 Pontedera, Italy; Department of Mechanical and Aerospace Engineering, Politecnico di Torino, Corso Duca degli Abruzzi 24, 10129 Torino, Italy. Electronic address: gianni.ciofani@iit.it.
authors.forename: Gianni
authors.initials: G
authors.lastname: Ciofani
mesh_terms: D000975:Antioxidants / Q000008:administration & dosage / Q000737:chemistry*; D001672:Biocompatible Materials / Q000008:administration & dosage / Q000737:chemistry; D002478:Cells, Cultured; D002563:Cerium / Q000008:administration & dosage / Q000737:chemistry*; D005780:Gelatin / Q000008:administration & dosage / Q000737:chemistry*; D006801:Humans; D053761:Nanocomposites / Q000008:administration & dosage / Q000737:chemistry*; D057139:Nanofibers / Q000008:administration & dosage / Q000737:chemistry*; D053758:Nanoparticles / Q000008:administration & dosage / Q000737:chemistry; D009416:Nerve Regeneration / Q000187:drug effects*; D009417:Nerve Tissue / Q000187:drug effects / Q000378:metabolism; D016501:Neurites / Q000187:drug effects / Q000378:metabolism; D009474:Neurons / Q000187:drug effects / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism; D044968:Regenerative Medicine / Q000379:methods; D023822:Tissue Engineering / Q000379:methods; D054457:Tissue Scaffolds
chemical_list: D000975:Antioxidants; D001672:Biocompatible Materials; D017382:Reactive Oxygen Species; D002563:Cerium; C030583:ceric oxide; D005780:Gelatin
keywords: Antioxidant interfaces; Axonal guidance; Cerium oxide nanoparticles; Electrospinning; Gelatin; Neuronal tissue engineering