title: Combined De-Algination Process as a Fractionation Strategy for Valorization of Brown Macroalga Saccharina japonica.
abstract: A combined process, de-algination followed by enzymatic saccharification, was designed to produce alginate and glucose from Saccharina japonica consecutively. The process conditions of de-algination were optimized separately for each stage of acidification and alkaline extraction. Collectively, the de-algination yield was 70.1% under the following optimized conditions: 2.4 wt% of Na2CO3, 70 °C, and 100 min with the acidified S. japonica immersed in a 0.5 wt% H2SO4solution for 2 h at room temperature. The glucan content in the de-alginated S. japonica increased to 38.0%, which was approximately fivefold higher than that of the raw S. japonica. The enzymatic hydrolysis of the de-alginated S. japonica almost completed in 9 h, affording 5.2 g (96.8% of glucan digestibility) of glucose at a de-alginated S. japonica loading of 14.2 g.
authors.affiliation: R&D Center, SugarEn Co., Ltd, Cheonan, Chungnam, 31116, South Korea.
authors.forename: Hyun Jin
authors.initials: HJ
authors.lastname: Ryu
authors.affiliation: Department of Applied Chemical Engineering, Dankook University, Cheonan, Chungnam, 31116, South Korea. kkoh@dankook.ac.kr.
authors.forename: Kyeong Keun
authors.initials: KK
authors.lastname: Oh
mesh_terms: D000464:Alginates / Q000737:chemistry / Q000302:isolation & purification*; D002254:Carbonates / Q000737:chemistry; D005936:Glucans / Q000737:chemistry; D005947:Glucose / Q000737:chemistry / Q000302:isolation & purification*; D020723:Glucuronic Acid / Q000737:chemistry / Q000302:isolation & purification; D006603:Hexuronic Acids / Q000737:chemistry / Q000302:isolation & purification; D006863:Hydrogen-Ion Concentration; D006867:Hydrolases / Q000737:chemistry; D006868:Hydrolysis; D007700:Kinetics; D007795:Laminaria / Q000737:chemistry*; D013464:Sulfuric Acids / Q000737:chemistry
chemical_list: D000464:Alginates; D002254:Carbonates; D005936:Glucans; D006603:Hexuronic Acids; D013464:Sulfuric Acids; C005686:sodium carbonate; D020723:Glucuronic Acid; D006867:Hydrolases; D005947:Glucose; C033158:sulfuric acid
keywords: Alginate extraction; Biorefinery; Brown algae; Laminaria; Phycocolloids; Pretreatment