title: Recyclable Saccharomyces cerevisiae loaded nanofibrous mats with sandwich structure constructing via bio-electrospraying for heavy metal removal.
abstract: Biosorbents, such as algae and yeast, have been applied in heavy metal adsorption due to their low cost and efficacy. However, they cannot be recycled and reused after direct application, which may cause a secondary pollution. In this study, we used bio-electrospraying technique to immobilize Saccharomyces cerevisiae (a byproduct from food fermentation) onto the surface of poly(ε-caprolactone)/chitosan/rectorite ternary composites based nanofibrous mats. This technique not only combined the advantages of both S. cerevisiae (cheap) and nanofibers (large surface area) in heavy metal removal, but also made biosorbents easy to recollect and reuse. Layer-by-layer structured nanofibrous mats were also fabricated by alternating electrospinning and bio-electrospraying for a couple of times and loaded more S. cerevisiae for enhancing heavy metal biosorption. The morphology of S. cerevisiae loaded nanofibrous mats with different numbers of layers was observed. Biosorption assay was performed on PbNO3solution under different pH values, contact time, initial concentrations of Pb2+and biosorbents weights, at last the elemental composition was measured before and after biosorption. The results showed that S. cerevisiae loaded nanofibrous mats had a biosorption capacity of Pb2+up to 238mg/g. Desorption assay indicated that these mats were reusable and maintained high biosorption capacity after three biosorption-desorption cycles.
authors.affiliation: Hubei International Scientific and Technological Cooperation Base of Sustainable Resource and Energy, Hubei Key Lab. of Biomass Resource Chemistry and Environmental Biotechnology, School of Resource and Environmental Science, Wuhan University, Wuhan 430079, China; Department of Biomedical Engineering, Texas A&M University, College Station, TX 77843, USA.
authors.forename: Shangjing
authors.initials: S
authors.lastname: Xin
authors.affiliation: Hubei International Scientific and Technological Cooperation Base of Sustainable Resource and Energy, Hubei Key Lab. of Biomass Resource Chemistry and Environmental Biotechnology, School of Resource and Environmental Science, Wuhan University, Wuhan 430079, China.
authors.forename: Zhaoyang
authors.initials: Z
authors.lastname: Zeng
authors.affiliation: School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
authors.forename: Xue
authors.initials: X
authors.lastname: Zhou
authors.affiliation: Department of Occupational & Environmental Health, School of Public Health, Fourth Military Medical University, Xi'an 710032, China.
authors.forename: Wenjing
authors.initials: W
authors.lastname: Luo
authors.affiliation: Hubei International Scientific and Technological Cooperation Base of Sustainable Resource and Energy, Hubei Key Lab. of Biomass Resource Chemistry and Environmental Biotechnology, School of Resource and Environmental Science, Wuhan University, Wuhan 430079, China.
authors.forename: Xiaowen
authors.initials: X
authors.lastname: Shi
authors.affiliation: Department of Chemical and Biological Engineering, Iowa State University, Ames, IA 50014, USA.
authors.forename: Qun
authors.initials: Q
authors.lastname: Wang
authors.affiliation: Hubei International Scientific and Technological Cooperation Base of Sustainable Resource and Energy, Hubei Key Lab. of Biomass Resource Chemistry and Environmental Biotechnology, School of Resource and Environmental Science, Wuhan University, Wuhan 430079, China. Electronic address: hbdeng@whu.edu.cn.
authors.forename: Hongbing
authors.initials: H
authors.lastname: Deng
authors.affiliation: Hubei International Scientific and Technological Cooperation Base of Sustainable Resource and Energy, Hubei Key Lab. of Biomass Resource Chemistry and Environmental Biotechnology, School of Resource and Environmental Science, Wuhan University, Wuhan 430079, China.
authors.forename: Yumin
authors.initials: Y
authors.lastname: Du
mesh_terms: D000327:Adsorption; D018914:Cells, Immobilized / Q000737:chemistry*; D019216:Metals, Heavy / Q000737:chemistry*; D057139:Nanofibers / Q000737:chemistry*; D012441:Saccharomyces cerevisiae / Q000737:chemistry*; D018508:Water Purification / Q000379:methods
chemical_list: D019216:Metals, Heavy
keywords: Layer-by-layer immobilization; Nanofibrous mats; Recyclable removal of heavy metal; Saccharomyces cerevisiae