title: CD103+CD11b-salivary gland dendritic cells have antigen cross-presenting capacity.
abstract: Dendritic cells (DCs) in lymphoid and non-lymphoid tissues are professional antigen-presenting cells that are essential for effective immunity and tolerance. However, the presence and characteristics of DCs in steady-state salivary glands (SGs) currently remain unknown. We herein identified CD64-CD11c+classical DCs (cDCs) as well as CD64+macrophages among CD45+MHC class II+antigen-presenting cells in steady-state murine SGs. SG cDCs were divided into CD103+CD11b-and CD103-CD11b+cDCs. CD103+CD11b-cDCs expressed XCR1, CLEC9A, and interferon regulatory factor 8, whereas CD103-CD11b+cDCs strongly expressed CD172a. Both cDC subsets in SGs markedly expanded in response to the Flt3 ligand (Flt3L), were replenished by bone marrow-derived precursors, and differentiated from common DC precursors, but not monocytes. Furthermore, ovalbumin-pulsed SG CD103+CD11b-cDCs induced the proliferation of naïve ovalbumin-specific CD8+T cells and production of interferon-γ from proliferating T cells. SG CD103+CD11b-cDCs expanded by Flt3L in vivo exhibited the same properties. These results indicate that bona fide cDCs reside in steady-state murine SGs and cDCs with the CD103+CD11b-phenotype possess antigen cross-presenting capacity. Moreover, Flt3L enhances protective immunity by expanding cDCs. Taken together, SG cDCs might play an important role in maintaining immune homeostasis in the tissues.
authors.affiliation: Division of Oral Immunology, Department of Oral Biology, Tohoku University Graduate School of Dentistry, Sendai, Japan.|Division of Oral Diagnosis, Department of Oral Medicine and Surgery, Tohoku University Graduate School of Dentistry, Sendai, Japan.
authors.forename: Lu
authors.initials: L
authors.lastname: Lu
authors.affiliation: Division of Oral Immunology, Department of Oral Biology, Tohoku University Graduate School of Dentistry, Sendai, Japan.|Division of Pediatric Dentistry, Department of Oral Health and Development Sciences, Tohoku University Graduate School of Dentistry, Sendai, Japan.
authors.forename: Yukinori
authors.initials: Y
authors.lastname: Tanaka
authors.affiliation: Department of Microbiology and Immunology, Tohoku University Graduate School of Medicine, Sendai, Japan.
authors.forename: Naoto
authors.initials: N
authors.lastname: Ishii
authors.affiliation: Division of Oral Diagnosis, Department of Oral Medicine and Surgery, Tohoku University Graduate School of Dentistry, Sendai, Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Sasano
authors.affiliation: Division of Oral Immunology, Department of Oral Biology, Tohoku University Graduate School of Dentistry, Sendai, Japan.
authors.forename: Shunji
authors.initials: S
authors.lastname: Sugawara
mesh_terms: D000818:Animals; D015703:Antigens, CD / Q000378:metabolism; D039481:CD11b Antigen / Q000378:metabolism; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D002454:Cell Differentiation; D049109:Cell Proliferation; D002478:Cells, Cultured; D045142:Cross-Priming; D003713:Dendritic Cells / Q000502:physiology*; D005260:Female; D039001:Integrin alpha Chains / Q000378:metabolism; D007371:Interferon-gamma / Q000378:metabolism; D008213:Lymphocyte Activation; D008264:Macrophages / Q000502:physiology*; D051379:Mice; D008810:Mice, Inbred C57BL; D017452:Receptors, IgG / Q000378:metabolism; D012469:Salivary Glands / Q000166:cytology*; D051941:fms-Like Tyrosine Kinase 3 / Q000276:immunology
chemical_list: D015703:Antigens, CD; D039481:CD11b Antigen; D039001:Integrin alpha Chains; D017452:Receptors, IgG; C091367:alpha E integrins; D007371:Interferon-gamma; C497971:Flt3 protein, mouse; D051941:fms-Like Tyrosine Kinase 3
keywords: Cross-presentation; Dendritic cells; Host defense; Immune homeostasis; Progenitors; Salivary glands