title: Isolation and characterization of LcSAP, a Leymus chinensis gene which enhances the salinity tolerance of Saccharomyces cerevisiae.
abstract: A number of members of the SAP ("stress-associated protein") gene family have been implicated in the plant stress response. Here, a SAP gene has been isolated using PCR RACE from the perennial grass Leymus chinensis, a species which has reputation for ecological adaptability. The 17.6 kDa LcSAP product comprised 161 residues, including both an A20 domain and an AN1 domain, a feature of type I SAPs. Using a semi-quantitative RT-PCR assay to profile its transcription, it was shown that LcSAP was more strongly transcribed in the leaf than in the root under control conditions. The level of LcSAP transcription began to rise 6 h after the plant's exposure to 400 mM NaCl, and the abundance of transcript remained stable for at least 24 h. Exposing the plant to 100 mM Na2CO3also induced LcSAP transcription, but the abundance of SAP transcript faded after 6 h. When LcSAP was introduced into yeast cells, the transgenic cells grew better than wild type ones when the medium contained 1.4 M NaCl. The ability of LcSAP to respond to salinity stress in yeast suggests that it also makes a contribution to the stress tolerance shown by L. chinensis.
authors.affiliation: State Key Laboratory of Soil Erosion and Dryland Farming on the Loess Plateau, Northwest A&F University, Yangling, 712100, People's Republic of China.|College of Life Sciences, Northwest A&F University, Yangling, 712100, People's Republic of China.
authors.forename: Jingying
authors.initials: J
authors.lastname: Liu
authors.affiliation: College of Life Sciences, Northwest A&F University, Yangling, 712100, People's Republic of China.
authors.forename: Xiangna
authors.initials: X
authors.lastname: Yang
authors.affiliation: College of Life Sciences, Northwest A&F University, Yangling, 712100, People's Republic of China.
authors.forename: Xizhe
authors.initials: X
authors.lastname: Yang
authors.affiliation: College of Life Sciences, Northwest A&F University, Yangling, 712100, People's Republic of China.
authors.forename: Mingyue
authors.initials: M
authors.lastname: Xu
authors.affiliation: College of Life Sciences, Northwest A&F University, Yangling, 712100, People's Republic of China.
authors.forename: Jie
authors.initials: J
authors.lastname: Liu
authors.affiliation: College of Life Sciences, Northwest A&F University, Yangling, 712100, People's Republic of China.
authors.forename: Mengmeng
authors.initials: M
authors.lastname: Xue
authors.affiliation: College of Life Sciences, Northwest A&F University, Yangling, 712100, People's Republic of China. mapengda@163.com.
authors.forename: Pengda
authors.initials: P
authors.lastname: Ma
mesh_terms: D003001:Cloning, Molecular / Q000379:methods*; D018506:Gene Expression Regulation, Plant; D010802:Phylogeny; D018515:Plant Leaves / Q000235:genetics; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D018517:Plant Roots / Q000235:genetics; D006109:Poaceae / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000254:growth & development*; D055049:Salt Tolerance; D012965:Sodium Chloride / Q000494:pharmacology; D013312:Stress, Physiological; D014018:Tissue Distribution; D014170:Transformation, Genetic
chemical_list: D010940:Plant Proteins; D012965:Sodium Chloride
keywords: Leymus chinensis; Saccharomyces cerevisiae; Salinity; Stress associated protein