title: Molecular characterization of a novel ssRNA ourmia-like virus from the rice blast fungus Magnaporthe oryzae.
abstract: In this study we characterize a novel positive and single stranded RNA (ssRNA) mycovirus isolated from the rice field isolate of Magnaporthe oryzae Guy11. The ssRNA contains a single open reading frame (ORF) of 2,373 nucleotides in length and encodes an RNA-dependent RNA polymerase (RdRp) closely related to ourmiaviruses (plant viruses) and ourmia-like mycoviruses. Accordingly, we name this virus Magnaporthe oryzae ourmia-like virus 1 (MOLV1). Although phylogenetic analysis suggests that MOLV1 is closely related to ourmia and ourmia-like viruses, it has some features never reported before within the Ourmiavirus genus. 3' RLM-RACE (RNA ligase-mediated rapid amplification of cDNA ends) and extension poly(A) tests (ePAT) suggest that the MOLV1 genome contains a poly(A) tail whereas the three cytosine and the three guanine residues present in 5' and 3' untranslated regions (UTRs) of ourmia viruses are not observed in the MOLV1 sequence. The discovery of this novel viral genome supports the hypothesis that plant pathogenic fungi may have acquired this type of viruses from their host plants.
authors.affiliation: Centro de Biotecnología y Genómica de Plantas, Universidad Politécnica de Madrid (UPM), Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA Campus Montegancedo UPM), Pozuelo de Alarcón, 28223, Madrid, Spain.|Departamento de Biotecnología-Biología Vegetal, Escuela Técnica Superior de Ingeniería Agronómica, Alimentaria y de Biosistemas, UPM, 28040, Madrid, Spain.
authors.forename: Adriana
authors.initials: A
authors.lastname: Illana
authors.affiliation: Centro de Biotecnología y Genómica de Plantas, Universidad Politécnica de Madrid (UPM), Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA Campus Montegancedo UPM), Pozuelo de Alarcón, 28223, Madrid, Spain.|Departamento de Biotecnología-Biología Vegetal, Escuela Técnica Superior de Ingeniería Agronómica, Alimentaria y de Biosistemas, UPM, 28040, Madrid, Spain.
authors.forename: Marco
authors.initials: M
authors.lastname: Marconi
authors.affiliation: Centro de Biotecnología y Genómica de Plantas, Universidad Politécnica de Madrid (UPM), Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA Campus Montegancedo UPM), Pozuelo de Alarcón, 28223, Madrid, Spain.|Departamento de Biotecnología-Biología Vegetal, Escuela Técnica Superior de Ingeniería Agronómica, Alimentaria y de Biosistemas, UPM, 28040, Madrid, Spain.
authors.forename: Julio
authors.initials: J
authors.lastname: Rodríguez-Romero
authors.affiliation: School of Biological Sciences, University of East Anglia, Norwich Research Park, Norwich, NR4 7TJ, UK.
authors.forename: Ping
authors.initials: P
authors.lastname: Xu
authors.affiliation: School of Biological Sciences, University of East Anglia, Norwich Research Park, Norwich, NR4 7TJ, UK.
authors.forename: Tamas
authors.initials: T
authors.lastname: Dalmay
authors.affiliation: Centro de Biotecnología y Genómica de Plantas, Universidad Politécnica de Madrid (UPM), Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA Campus Montegancedo UPM), Pozuelo de Alarcón, 28223, Madrid, Spain.|Departamento de Biotecnología-Biología Vegetal, Escuela Técnica Superior de Ingeniería Agronómica, Alimentaria y de Biosistemas, UPM, 28040, Madrid, Spain.
authors.forename: Mark D
authors.initials: MD
authors.lastname: Wilkinson
authors.affiliation: Centro de Biotecnología y Genómica de Plantas, Universidad Politécnica de Madrid (UPM), Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA Campus Montegancedo UPM), Pozuelo de Alarcón, 28223, Madrid, Spain.|Departamento de Biotecnología-Biología Vegetal, Escuela Técnica Superior de Ingeniería Agronómica, Alimentaria y de Biosistemas, UPM, 28040, Madrid, Spain.
authors.forename: Maria Ángeles
authors.initials: MÁ
authors.lastname: Ayllón
authors.affiliation: Centro de Biotecnología y Genómica de Plantas, Universidad Politécnica de Madrid (UPM), Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA Campus Montegancedo UPM), Pozuelo de Alarcón, 28223, Madrid, Spain. ane.sesma@upm.es.|Departamento de Biotecnología-Biología Vegetal, Escuela Técnica Superior de Ingeniería Agronómica, Alimentaria y de Biosistemas, UPM, 28040, Madrid, Spain. ane.sesma@upm.es.
authors.forename: Ane
authors.identifier: http://orcid.org/0000-0003-3982-8932
authors.initials: A
authors.lastname: Sesma
mesh_terms: D020413:3' Untranslated Regions; D000595:Amino Acid Sequence; D000067148:Fungal Viruses / Q000737:chemistry / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D016679:Genome, Viral; D020082:Magnaporthe / Q000821:virology*; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D012275:Oryza / Q000382:microbiology*; D010802:Phylogeny; D010935:Plant Diseases / Q000382:microbiology*; D012328:RNA Viruses / Q000737:chemistry / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D012367:RNA, Viral / Q000235:genetics; D016415:Sequence Alignment; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics
chemical_list: D020413:3' Untranslated Regions; D012367:RNA, Viral; D014764:Viral Proteins