title: Combined therapy using low level laser and chitosan-policaju hydrogel for wound healing.
abstract: We have evaluated the effect of POLI-CHI hydrogel based on policaju (POLI) from cashew tree (Anacardium occidentale L.) gum and chitosan (CHI), associated or not with Low level laser therapy (LLLT), in wound healing. Sixty male Wistar rats were assigned into four groups: POLI-CHI hydrogel (H); LLLT (L); POLI-CHI with LLLT (HL) and saline control (C). Macroscopic evaluations were carried out using clinical observations and area measurements, as well as microscopic analysis by histological criteria. H and HL presented more esthetical scar tissue and larger wound contraction compared to C. Histopathological analyzes showed: stronger presence of fibrin-leukocyte crust in L and HL at day 3; stronger collagen presence in H, L and HL; weak presence of focal necrosis at 7 and 14days in H; weak neutrophilic exudate in H, L and HL; regression of the vascular neoformation at 7days in H, and modulation of the same in L and HL. These results demonstrated that POLI-CHI contributed to more efficient healing process and modulation of the inflammation; furthermore, the combined use with LLLT subtle potentiated this process.
authors.affiliation: Departamento de Bioquímica and Laboratório de Imunopatologia Keizo Asami, Universidade Federal de Pernambuco (UFPE), Av. Prof. Moraes Rego, s/n, Cidade Universitária, CEP: 50670-420, Recife, PE, Brazil.
authors.forename: Adelmo C
authors.initials: AC
authors.lastname: Aragão-Neto
authors.affiliation: Departamento de Bioquímica and Laboratório de Imunopatologia Keizo Asami, Universidade Federal de Pernambuco (UFPE), Av. Prof. Moraes Rego, s/n, Cidade Universitária, CEP: 50670-420, Recife, PE, Brazil.
authors.forename: Paulo A G
authors.initials: PA
authors.lastname: Soares
authors.affiliation: Departamento de Bioquímica and Laboratório de Imunopatologia Keizo Asami, Universidade Federal de Pernambuco (UFPE), Av. Prof. Moraes Rego, s/n, Cidade Universitária, CEP: 50670-420, Recife, PE, Brazil.
authors.forename: Maria H M
authors.initials: MH
authors.lastname: Lima-Ribeiro
authors.affiliation: Departamento de Odontologia Preventiva, UFPE, Av. Prof. Moraes Rego, 1235, Cidade Universitária, CEP: 50670-901, Recife, PE, Brazil.
authors.forename: Elaine J A
authors.initials: EJ
authors.lastname: Carvalho
authors.affiliation: Departamento de Bioquímica and Laboratório de Imunopatologia Keizo Asami, Universidade Federal de Pernambuco (UFPE), Av. Prof. Moraes Rego, s/n, Cidade Universitária, CEP: 50670-420, Recife, PE, Brazil.
authors.forename: Maria T S
authors.initials: MT
authors.lastname: Correia
authors.affiliation: Departamento de Bioquímica and Laboratório de Imunopatologia Keizo Asami, Universidade Federal de Pernambuco (UFPE), Av. Prof. Moraes Rego, s/n, Cidade Universitária, CEP: 50670-420, Recife, PE, Brazil. Electronic address: mgcc1954@gmail.com.
authors.forename: Maria G
authors.initials: MG
authors.lastname: Carneiro-da-Cunha
mesh_terms: D031021:Anacardium / Q000737:chemistry; D000818:Animals; D048271:Chitosan / Q000737:chemistry* / Q000494:pharmacology*; D003131:Combined Modality Therapy; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000737:chemistry*; D028022:Low-Level Light Therapy; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D014945:Wound Healing / Q000187:drug effects* / Q000528:radiation effects*
chemical_list: D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D048271:Chitosan
keywords: Anacardium occidentale L.; Chitosan; Cutaneous dressing; Polysaccharides