title: Inflammatory Cytokines and BDNF Response to High-Intensity Intermittent Exercise: Effect the Exercise Volume.
abstract: The purpose of this study was to compare the effects of two similar high-intensity intermittent exercises (HIIE) but different volume 1.25 km (HIIE1.25) and 2.5 km (HIIE2.5) on inflammatory and BDNF responses. Ten physically active male subjects (age 25.22 ± 1.74 years, body mass 78.98 ± 7.31 kg, height 1.78 ± 0.06 m, VO2peak59.94 ± 9.38 ml·kg·min-1) performed an incremental treadmill exercise test and randomly completed two sessions of HIIE on a treadmill (1:1 min at vVO2maxwith passive recovery). Blood samples were collected at rest, immediately and 60-min after the exercise sessions. Serum was analyzed for glucose, lactate, IL-6, IL-10, and BDNF levels. Blood lactate concentrations was higher immediately post-exercise compared to rest (HIIE1.25: 1.69 ± 0.26-7.78 ± 2.09 mmol·L-1, and HIIE2.5: 1.89 ± 0.26-7.38 ± 2.57 mmol·L-1,p< 0.0001). Glucose concentrations did not present changes under the different conditions, however, levels were higher 60-min post-exercise than at rest only in the HIIE1.25 condition (rest: 76.80 ± 11.14-97.84 ± 24.87 mg·dL-1,p< 0.05). BDNF level increased immediately after exercise in both protocols (HIIE1.25: 9.71 ± 306-17.86 ± 8.59 ng.mL-1, and HIIE2.5: 11.83 ± 5.82-22.84 ± 10.30 ng.mL-1). Although both exercises increased IL-6, level percent between rest and immediately after exercise was higher in the HIIE2.5 than HIIE1.25 (30 and 10%;p= 0.014, respectively). Moreover, IL-10 levels percent increase between immediately and 60-min post-exercise was higher in HIIE2.5 than HIIE1.25 (37 and 10%;p= 0.012, respectively). In conclusion, both HIIE protocols with the same intensity were effective to increase BDNF and IL-6 levels immediately after exercise while only IL-10 response was related to the durantion of exercise indicanting the importance of this exercise prescription variable.
authors.affiliation: Exercise and Immunometabolism Research Group, Departamento de Educação Física, Universidade Estadual Paulista - Presidente Prudente São Paulo, Brasil.
authors.forename: Carolina
authors.initials: C
authors.lastname: Cabral-Santos
authors.affiliation: Laboratório de Fisioterapia Desportiva, Faculdade de Ciências e Tecnologia, Departamento de Fisioterapia, Universidade Estadual Paulista - Presidente Prudente São Paulo, Brasil.
authors.forename: Carlos I M
authors.initials: CI
authors.lastname: Castrillón
authors.affiliation: Laboratório de Fisioterapia Desportiva, Faculdade de Ciências e Tecnologia, Departamento de Fisioterapia, Universidade Estadual Paulista - Presidente Prudente São Paulo, Brasil.
authors.forename: Rodolfo A T
authors.initials: RA
authors.lastname: Miranda
authors.affiliation: Exercise and Immunometabolism Research Group, Departamento de Educação Física, Universidade Estadual Paulista - Presidente Prudente São Paulo, Brasil.
authors.forename: Paula A
authors.initials: PA
authors.lastname: Monteiro
authors.affiliation: Exercise and Immunometabolism Research Group, Departamento de Educação Física, Universidade Estadual Paulista - Presidente Prudente São Paulo, Brasil.
authors.forename: Daniela S
authors.initials: DS
authors.lastname: Inoue
authors.affiliation: Exercise and Immunometabolism Research Group, Departamento de Educação Física, Universidade Estadual Paulista - Presidente PrudenteSão Paulo, Brasil; Department of Physical Education, Federal University of PernambucoRecife, Brazil.
authors.forename: Eduardo Z
authors.initials: EZ
authors.lastname: Campos
authors.affiliation: Exercise Physiology, Training and Training Therapy Research Group, Institute of Sport Science, University of Graz Graz, Austria.
authors.forename: Peter
authors.initials: P
authors.lastname: Hofmann
authors.affiliation: Exercise and Immunometabolism Research Group, Departamento de Educação Física, Universidade Estadual Paulista - Presidente Prudente São Paulo, Brasil.
authors.forename: Fábio S
authors.initials: FS
authors.lastname: Lira
keywords: cytokines; high-intensity intermittent exercise; inflammatory response; low-grade inflammation; metabolic response