title: Complete sequence and variability of a new subgroup B nepovirus infecting potato in central Peru.
abstract: The complete bipartite genome (RNA1 and RNA2) of a new nepovirus infecting potato was obtained using small RNA sequencing and assembly complemented by Sanger sequencing. Each RNA encodes a single polyprotein, flanked by 5' and 3' untranslate regions (UTR) and followed by a poly (A) tail. The putative polyproteins encoded by RNA1 and RNA2 had sets of motifs which are characteristic of viruses in the genus Nepovirus. Sequence comparisons using the Pro-Pol region and the coat protein, including phylogenetic analysis of these regions, showed closest relationships with nepoviruses. The data obtained support the taxonomical status of this new virus (putative named Potato virus B, PVB) as a member of the genus Nepovirus, subgroup B.
authors.affiliation: International Potato Center (CIP), Apartado 1558, Lima 12, Peru.|Facultad de Ciencias Biológicas, Programa de Biología Molecular, Universidad Nacional Mayor de San Marcos, Lima 11, Peru.
authors.forename: Joao
authors.initials: J
authors.lastname: De Souza
authors.affiliation: International Potato Center (CIP), Apartado 1558, Lima 12, Peru.
authors.forename: Giovanna
authors.initials: G
authors.lastname: Müller
authors.affiliation: International Potato Center (CIP), Apartado 1558, Lima 12, Peru.
authors.forename: Wilmer
authors.initials: W
authors.lastname: Perez
authors.affiliation: International Potato Center (CIP), Apartado 1558, Lima 12, Peru.|International Center for Tropical Agriculture (CIAT), Apartado Aéreo 6713, Cali, Colombia.
authors.forename: Wilmer
authors.initials: W
authors.lastname: Cuellar
authors.affiliation: International Potato Center (CIP), Apartado 1558, Lima 12, Peru. j.kreuze@cgiar.org.
authors.forename: Jan
authors.initials: J
authors.lastname: Kreuze
mesh_terms: D001483:Base Sequence; D014644:Genetic Variation; D016679:Genome, Viral; D008969:Molecular Sequence Data; D017860:Nepovirus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D010568:Peru; D010802:Phylogeny; D010935:Plant Diseases / Q000821:virology*; D012367:RNA, Viral / Q000235:genetics; D017422:Sequence Analysis, DNA; D011198:Solanum tuberosum / Q000821:virology*; D014764:Viral Proteins / Q000235:genetics
chemical_list: D012367:RNA, Viral; D014764:Viral Proteins