title: Intratubular disinfection with tri-antibiotic and calcium hydroxide pastes.
abstract: OBJECTIVE
  The aim of this study was to compare the in vitro intradentinal antimicrobial ability of the calcium hydroxide and tri-antibiotic pastes.
  
  
  METHODS
  Standard bovine dentin tubes were sterilized and then infected with Enterococcus faecalis by a new contamination protocol of great depths of dentin. The specimens were filled with the medications, divided into two test-groups: calcium hydroxide (Group 1) and tri-antibiotic (Group 2) pastes. After 15 days, the teeth were evaluated by microbiological culture and confocal laser scanning microscopy (CLSM) with viability dye assay LIVE/DEAD inside dentinal tubules. In experiment of culture, the bacterial collection of the dentin fragments was done for counting the colony-forming units.
  
  
  RESULTS
  The tri-antibiotic paste had a slightly greater antimicrobial effect; however, there was no statistical difference between the groups.
  
  
  CONCLUSIONS
  It was concluded that the tri-antibiotic paste and the calcium hydroxide paste exercise the same effect on intra-tubular decontamination against E. faecalis. So, due the multiples advantages, the calcium hydroxide paste can be the choice for dentinal decontamination in regenerative procedures.
authors.affiliation: a Department of Dentistry, Endodontics and Dental Materials , Bauru Dental School, University of São Paulo , Bauru , SP , Brazil.
authors.forename: Thais Cristina
authors.initials: TC
authors.lastname: Pereira
authors.affiliation: a Department of Dentistry, Endodontics and Dental Materials , Bauru Dental School, University of São Paulo , Bauru , SP , Brazil.
authors.forename: Layla Reginna Silva Munhoz De
authors.initials: LR
authors.lastname: Vasconcelos
authors.affiliation: a Department of Dentistry, Endodontics and Dental Materials , Bauru Dental School, University of São Paulo , Bauru , SP , Brazil.
authors.forename: Marcia Sirlene Zardin
authors.initials: MS
authors.lastname: Graeff
authors.affiliation: a Department of Dentistry, Endodontics and Dental Materials , Bauru Dental School, University of São Paulo , Bauru , SP , Brazil.
authors.forename: Marco Antonio Hungaro
authors.initials: MA
authors.lastname: Duarte
authors.affiliation: a Department of Dentistry, Endodontics and Dental Materials , Bauru Dental School, University of São Paulo , Bauru , SP , Brazil.
authors.forename: Clóvis Monteiro
authors.initials: CM
authors.lastname: Bramante
authors.affiliation: a Department of Dentistry, Endodontics and Dental Materials , Bauru Dental School, University of São Paulo , Bauru , SP , Brazil.
authors.forename: Flaviana Bombarda De
authors.initials: FB
authors.lastname: Andrade
mesh_terms: D000818:Animals; D000890:Anti-Infective Agents / Q000494:pharmacology*; D002126:Calcium Hydroxide / Q000494:pharmacology*; D002417:Cattle; D003804:Dentin / Q000382:microbiology; D004203:Disinfection / Q000379:methods*; D013293:Enterococcus faecalis / Q000187:drug effects*; D050296:Microbial Viability / Q000187:drug effects; D009824:Ointments / Q000008:administration & dosage; D012388:Root Canal Irrigants / Q000494:pharmacology; D040521:Subcutaneous Tissue / Q000187:drug effects*
chemical_list: D000890:Anti-Infective Agents; D009824:Ointments; D012388:Root Canal Irrigants; D002126:Calcium Hydroxide
keywords: Antibiotic; Enterococcus faecalis; calcium hydroxide; microscopy