title: Pomegranate-Derived Polyphenols Reduce Reactive Oxygen Species Production via SIRT3-Mediated SOD2 Activation.
abstract: Pomegranate-derived polyphenols are expected to prevent life-style related diseases. In this study, we evaluated the ability of 8 pomegranate-derived polyphenols, along with other polyphenols, to augmentSIRT3, a mammalianSIR2homolog localized in mitochondria. We established a system for screening foods/food ingredients that augment theSIRT3promoter in Caco-2 cells and identified 3 SIRT3-augmenting pomegranate-derived polyphenols (eucalbanin B, pomegraniin A, and eucarpanin T1). Among them, pomegraniin A activated superoxide dismutase 2 (SOD2) through SIRT3-mediated deacetylation, thereby reducing intracellular reactive oxygen species. The other SIRT3-augmenting polyphenols tested also activated SOD2, suggesting antioxidant activity. Our findings clarify the underlying mechanisms involved in the antioxidant activity of pomegraniin A.
authors.affiliation: Graduate School of Systems Life Sciences, Kyushu University, 6-10-1 Hakozaki, Higashi-ku, Fukuoka 812-8581, Japan.
authors.forename: Chong
authors.initials: C
authors.lastname: Zhao
authors.affiliation: Graduate School of Bioscience & Biotechnology, Kyushu University, 6-10-1 Hakozaki, Higashi-ku, Fukuoka 812-8581, Japan.
authors.forename: Takenori
authors.initials: T
authors.lastname: Sakaguchi
authors.affiliation: Graduate School of Systems Life Sciences, Kyushu University, 6-10-1 Hakozaki, Higashi-ku, Fukuoka 812-8581, Japan.
authors.forename: Kosuke
authors.initials: K
authors.lastname: Fujita
authors.affiliation: Faculty of Health and Welfare Science, Okayama Prefectural University, 111 Kuboki, Soja, Okayama 719-1197, Japan.
authors.forename: Hideyuki
authors.initials: H
authors.lastname: Ito
authors.affiliation: Morishita Jintan Co., Ltd., 2-11-1 Tsudayamate, Hirakata, Osaka 573-0128, Japan.
authors.forename: Norihisa
authors.initials: N
authors.lastname: Nishida
authors.affiliation: Morishita Jintan Co., Ltd., 2-11-1 Tsudayamate, Hirakata, Osaka 573-0128, Japan.
authors.forename: Akifumi
authors.initials: A
authors.lastname: Nagatomo
authors.affiliation: Morishita Jintan Co., Ltd., 2-11-1 Tsudayamate, Hirakata, Osaka 573-0128, Japan.
authors.forename: Yukimasa
authors.initials: Y
authors.lastname: Tanaka-Azuma
authors.affiliation: Graduate School of Systems Life Sciences, Kyushu University, 6-10-1 Hakozaki, Higashi-ku, Fukuoka 812-8581, Japan; Graduate School of Bioscience & Biotechnology, Kyushu University, 6-10-1 Hakozaki, Higashi-ku, Fukuoka 812-8581, Japan; Faculty of Agriculture, Kyushu University, 6-10-1 Hakozaki, Higashi-ku, Fukuoka 812-8581, Japan.
authors.forename: Yoshinori
authors.identifier: 0000-0002-9678-9602
authors.initials: Y
authors.lastname: Katakura
mesh_terms: D029561:Lythraceae / Q000378:metabolism*; D018384:Oxidative Stress; D059808:Polyphenols / Q000378:metabolism*; D017382:Reactive Oxygen Species; D037761:Sirtuins / Q000235:genetics*; D013482:Superoxide Dismutase / Q000378:metabolism*
chemical_list: D059808:Polyphenols; D017382:Reactive Oxygen Species; D013482:Superoxide Dismutase; D037761:Sirtuins