title: Protein regulation and Apoptotic induction in human breast carcinoma cells (MCF-7) through lectin from G. beauts.
abstract: Lectins are proteins that show a variety of biological activities. Nevertheless, information on lectin from Gluttonous beauts and their anticancer activities are very limited. In this study, we purified a lectin from hemolymph of G. beauts and identified its molecular weight to be 66kDa. The effect of lectin at different concentrations (μg/mL) on the cell growth and apoptosis were evaluated against MCF-7 and MCF-10A cells, whereas cytotoxicity to the MCF-7 cells mediated by lectin was observed and the mechanism of action of the lectin in including apoptosis in cancer cells via the intrinsic pathway was also proposed. The MCF-7 cells were employed for in vitro studies on cytotoxicity, induction of apoptosis and apoptotic DNA fragmentation. In MCF-10A cells lectin did not show any adverse effect even at higher concentration. Cell cycle analysis also showed a significant cell cycle arrest on selected cells after lectin treatment. Western blotting suggested that lectin up regulates the apoptotic protein expression in MCF-7 cells while it down regulates the level of Bcl-2 expression.
authors.affiliation: Proteomics and Molecular Cell Physiology Lab, Department of Zoology, School of Life Sciences, Bharathiar University, Coimbatore 641 046, TN, India. Electronic address: ponsprotein17@yahoo.com.
authors.forename: Thondhi
authors.initials: T
authors.lastname: Ponraj
authors.affiliation: Proteomics and Molecular Cell Physiology Lab, Department of Zoology, School of Life Sciences, Bharathiar University, Coimbatore 641 046, TN, India.
authors.forename: Manickam
authors.initials: M
authors.lastname: Paulpandi
authors.affiliation: Biomaterial Laboratory, School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai 200240, China.
authors.forename: Raju
authors.initials: R
authors.lastname: Vivek
authors.affiliation: Proteomics and Molecular Cell Physiology Lab, Department of Zoology, School of Life Sciences, Bharathiar University, Coimbatore 641 046, TN, India.
authors.forename: Karuppaiya
authors.initials: K
authors.lastname: Vimala
authors.affiliation: Department of Zoology, School of Life Sciences, Periyar University, Salem 636 011, TN, India. Electronic address: skperiyaruniv@gmail.com.
authors.forename: Soundarapandian
authors.initials: S
authors.lastname: Kannan
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000302:isolation & purification / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D060829:Arthropod Proteins / Q000302:isolation & purification / Q000494:pharmacology*; D001181:Arthropods / Q000737:chemistry*; D059447:Cell Cycle Checkpoints / Q000187:drug effects; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D053938:DNA Fragmentation / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D005260:Female; D015870:Gene Expression; D006458:Hemolymph / Q000737:chemistry*; D006801:Humans; D037102:Lectins / Q000302:isolation & purification / Q000494:pharmacology*; D061986:MCF-7 Cells; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000037:antagonists & inhibitors* / Q000235:genetics / Q000378:metabolism
chemical_list: D000970:Antineoplastic Agents; D060829:Arthropod Proteins; C000595707:BCL2 protein, human; D037102:Lectins; D019253:Proto-Oncogene Proteins c-bcl-2
keywords: Apoptosis; Cytotoxicity; G. beauts; Human breast cancer MCF-7 cells; Lectin protein