title: Identification of two substrates of FTS_1067 protein - An essential virulence factor of Francisella tularensis.
abstract: Francisella tularensis is a highly virulent intracellular pathogen with the capacity to infect a variety of hosts including humans. One of the most important proteins involved in F. tularensis virulence and pathogenesis is the protein DsbA. This protein is annotated as a lipoprotein with disulfide oxidoreductase/isomerase activity. Therefore, its interactions with different substrates, including probable virulence factors, to assist in their proper folding are anticipated. We aimed to use the immunopurification approach to find DsbA (gene locus FTS_1067) interacting partners in F. tularensis subsp. holarctica strain FSC200 and compare the identified substrates with proteins which were found in our previous comparative proteome analysis. As a result of our work two FTS_1067 substrates, D-alanyl-D-alanine carboxypeptidase family protein and HlyD family secretion protein, were identified. Bacterial two-hybrid systems were further used to test their relevance in confirming FTS_1067 protein interactions.
authors.affiliation: 1 Department of Molecular Pathology and Biology, Faculty of Military Health Sciences, University of Defence , Hradec Králové, Czech Republic.
authors.forename: Petra
authors.initials: P
authors.lastname: Spidlova
authors.affiliation: 1 Department of Molecular Pathology and Biology, Faculty of Military Health Sciences, University of Defence , Hradec Králové, Czech Republic.
authors.forename: Iva
authors.initials: I
authors.lastname: Senitkova
authors.affiliation: 1 Department of Molecular Pathology and Biology, Faculty of Military Health Sciences, University of Defence , Hradec Králové, Czech Republic.
authors.forename: Marek
authors.initials: M
authors.lastname: Link
authors.affiliation: 1 Department of Molecular Pathology and Biology, Faculty of Military Health Sciences, University of Defence , Hradec Králové, Czech Republic.
authors.forename: Jiri
authors.initials: J
authors.lastname: Stulik
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D005604:Francisella tularensis / Q000235:genetics / Q000378:metabolism* / Q000472:pathogenicity*; D008074:Lipoproteins / Q000235:genetics / Q000378:metabolism*; D011485:Protein Binding; D037521:Virulence Factors / Q000235:genetics / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D008074:Lipoproteins; D037521:Virulence Factors
keywords: FTS_1067 protein; Francisella tularensis; bacterial two-hybrid assay; immunopurification