title: Ferrocyanide-Mediated Photoreduction of Ferricytochrome C Utilized to Selectively Probe Non-native Conformations Induced by Binding to Cardiolipin-Containing Liposomes.
abstract: Ferricytochrome c binding to cardiolipin-containing liposomes produces a heterogeneous distribution of conformations comprising native-like and non-native misfolded proteins. We utilized the photoreduction of native ferricytochrome c in the presence of potassium ferrocyanide and resonance Raman spectroscopy to probe the population of native and misfolded cytochrome c on liposomes with 20 % tetraoleylcardiolipin (TOCL)/80 % dioleylphosphocholine (DOPC) and with 100 % TOCL as a function of TOCL concentration. Our data provided strong support for an earlier model, which predicts that the equilibrium between native and non-native conformations is shifted to the latter with decreasing protein occupation of liposomes.
authors.affiliation: Department of Chemistry, Drexel University, 3141 Chestnut Street, Philadelphia, PA, 19104, USA.
authors.forename: Dmitry
authors.initials: D
authors.lastname: Malyshka
authors.affiliation: Department of Chemistry, Drexel University, 3141 Chestnut Street, Philadelphia, PA, 19104, USA.
authors.forename: Reinhard
authors.identifier: http://orcid.org/0000-0001-5616-0722
authors.initials: R
authors.lastname: Schweitzer-Stenner
mesh_terms: D002308:Cardiolipins / Q000737:chemistry*; D045304:Cytochromes c / Q000737:chemistry*; D005295:Ferrocyanides / Q000737:chemistry*; D008027:Light; D008081:Liposomes / Q000737:chemistry* / Q000378:metabolism; D010084:Oxidation-Reduction; D010713:Phosphatidylcholines / Q000737:chemistry; D013059:Spectrum Analysis, Raman
chemical_list: D002308:Cardiolipins; D005295:Ferrocyanides; D008081:Liposomes; D010713:Phosphatidylcholines; D045304:Cytochromes c; C017251:1,2-oleoylphosphatidylcholine; C031835:potassium ferrocyanide
keywords: Raman spectroscopy; cardiolipin; cytochrome c; liposomes; photoreduction