title: Investigation of sulfonamides inhibition of carbonic anhydrase enzyme using multiphotometric and electrochemical techniques.
abstract: Two approaches for sulfonamides (SA's) determination, based on carbonic anhydrase enzyme inhibition have been investigated in this work. In the first method, nine different SA's have been screened using simultaneous multiphotometric measurement in multiwell plates. The sulfanilamide (SAD) showed significant inhibition compared to other sulfonamides. The carbonic anhydrase (CA) kinetic interactions reveal noncompetitive binding of SAD. Interferences from other inhibitors with enzyme were studied and the results showed very good selectivity toward SAD. In the second approach, an electrochemical enzyme inhibition biosensor, based on CA entrapped in a carbon paste electrode using carbon black nanoparticles and solid paraffin, was successfully applied to SAD measurements. Results from the quantitative analysis of SAD are discussed in terms of detection limit, linear range and sensitivity using multiphotometric and biosensor-based methods The biosensor developed was successfully applied to the determination of SAD at submicromolar levels and it is recommended for application for in situ analysis.
authors.affiliation: Laboratoire Analyses Chimiques et Biocapteurs, Faculté des Sciences et Techniques, Hassan II University of Casablanca, B.P.146, Mohammedia, Morocco.
authors.forename: Ilhame
authors.initials: I
authors.lastname: Bourais
authors.affiliation: Laboratoire Analyses Chimiques et Biocapteurs, Faculté des Sciences et Techniques, Hassan II University of Casablanca, B.P.146, Mohammedia, Morocco.
authors.forename: Sara
authors.initials: S
authors.lastname: Maliki
authors.affiliation: Laboratoire Analyses Chimiques et Biocapteurs, Faculté des Sciences et Techniques, Hassan II University of Casablanca, B.P.146, Mohammedia, Morocco.
authors.forename: Hasna
authors.initials: H
authors.lastname: Mohammadi
authors.affiliation: Laboratoire Analyses Chimiques et Biocapteurs, Faculté des Sciences et Techniques, Hassan II University of Casablanca, B.P.146, Mohammedia, Morocco. Electronic address: azizamine@yahoo.fr.
authors.forename: Aziz
authors.initials: A
authors.lastname: Amine
mesh_terms: D000818:Animals; D015374:Biosensing Techniques / Q000379:methods; D002257:Carbonic Anhydrase Inhibitors / Q000032:analysis / Q000494:pharmacology*; D002256:Carbonic Anhydrases / Q000378:metabolism; D055664:Electrochemical Techniques / Q000379:methods; D004795:Enzyme Stability; D006801:Humans; D006863:Hydrogen-Ion Concentration; D010783:Photometry / Q000379:methods; D053260:Soot; D000077145:Sulfanilamide; D013424:Sulfanilamides / Q000032:analysis / Q000494:pharmacology; D013449:Sulfonamides / Q000032:analysis / Q000494:pharmacology*; D013696:Temperature
chemical_list: D002257:Carbonic Anhydrase Inhibitors; D053260:Soot; D013424:Sulfanilamides; D013449:Sulfonamides; D000077145:Sulfanilamide; D002256:Carbonic Anhydrases
keywords: Carbonic anhydrase; Electrochemical biosensor; Enzyme inhibition; Multiphotometric assay; Sulfonamides