title: Synergistic effect of polyaniline coverage and surface microstructure on the inhibition of Pseudomonas aeruginosa biofilm formation.
abstract: Biofilm Formation is a survival strategy for microorganisms to adapt to their environment. Microbial cells in biofilm become tolerant and resistant to antibiotics and immune responses, increasing the difficulties for the clinical treatment of microbial infections. The surface chemistry and the micro/nano-topography of solid interfaces play a major role in mediating microorganism activity and adhesion. The effect of the surface chemical composition and topography on the adhesion and viability of Pseudomonas aeruginosa was studied. Polymeric (polyethylene terephthalate) surfaces were covered with a conducting polymer (polyaniline, PANI) film by in-situ polymerization and microstructured by Direct Laser Interference Patterning (DLIP). The viability of Pseudomonas aeruginosa on the different surfaces was investigated. The physicochemical properties of the surfaces were characterized by water contact angle measurements, scanning electron microscopy and atomic force microscopy. Bacterial biofilms were imaged by atomic force and scanning electron microscopies. The bacterial viability decreased on PANI compared with the substrate (polyethylene terephthalate) and it decreased even more upon micro-structuring the PANI films. In addition, the biofilm reduction could be improved using polymers with different chemical composition and/or the same polymer with different topographies. Both methods presented diminish the bacterial attachment and biofilm formation. These findings present a high impact related to materials for biomedical engineer applications regarding medical devices, as prostheses or catheters.
authors.affiliation: Departamento de Biología Molecular, Universidad Nacional de Río Cuarto-CONICET, Agencia Postal No. 3, X580BYA Río Cuarto, Argentina.
authors.forename: L A
authors.initials: LA
authors.lastname: Gallarato
authors.affiliation: Departamento de Química, Universidad Nacional de Río Cuarto-CONICET, Agencia Postal No. 3, X580BYA Río Cuarto, Argentina.
authors.forename: L E
authors.initials: LE
authors.lastname: Mulko
authors.affiliation: Departamento de Biología Molecular, Universidad Nacional de Río Cuarto-CONICET, Agencia Postal No. 3, X580BYA Río Cuarto, Argentina.
authors.forename: M S
authors.initials: MS
authors.lastname: Dardanelli
authors.affiliation: Departamento de Química, Universidad Nacional de Río Cuarto-CONICET, Agencia Postal No. 3, X580BYA Río Cuarto, Argentina.
authors.forename: C A
authors.initials: CA
authors.lastname: Barbero
authors.affiliation: Departamento de Química, Universidad Nacional de Río Cuarto-CONICET, Agencia Postal No. 3, X580BYA Río Cuarto, Argentina. Electronic address: dacevedo@ing.unrc.edu.ar.
authors.forename: D F
authors.initials: DF
authors.lastname: Acevedo
authors.affiliation: Departamento de Biología Molecular, Universidad Nacional de Río Cuarto-CONICET, Agencia Postal No. 3, X580BYA Río Cuarto, Argentina. Electronic address: eyslas@exa.unrc.edu.ar.
authors.forename: E I
authors.initials: EI
authors.lastname: Yslas
mesh_terms: D000814:Aniline Compounds / Q000737:chemistry*; D001422:Bacterial Adhesion; D001672:Biocompatible Materials / Q000737:chemistry; D018441:Biofilms; D057785:Catheters; D024881:Drug Resistance, Bacterial; D004864:Equipment and Supplies; D005840:Gentian Violet / Q000737:chemistry; D018625:Microscopy, Atomic Force; D008855:Microscopy, Electron, Scanning; D008856:Microscopy, Fluorescence; D011093:Polyethylene Terephthalates / Q000737:chemistry; D011550:Pseudomonas aeruginosa / Q000502:physiology*; D013499:Surface Properties
chemical_list: D000814:Aniline Compounds; D001672:Biocompatible Materials; D011093:Polyethylene Terephthalates; C416807:polyaniline; D005840:Gentian Violet
keywords: Adhesion; Antimicrobial; Microstructured surface; Polyaniline; Pseudomonas aeruginosa