title: Plug-and-playable fluorescent cell imaging modular toolkits using the bacterial superglue, SpyTag/SpyCatcher.
abstract: Simple plug-and-playable fluorescent cell imaging modular toolkits are established using the bacterial superglue SpyTag/SpyCatcher protein ligation system. A variety of affibody-fluorescent protein conjugates (AFPCs) are post-translationally generated via the isopeptide bond formation, and each AFPC effectively recognizes and binds to its targeting cells, visualizing them with selective colors on demand.
authors.affiliation: Department of Biological Sciences, School of Life Sciences, Ulsan National Institute of Science and Technology (UNIST), Ulsan, 44919, Korea. sabsab7@unist.ac.kr.
authors.forename: Hyojin
authors.initials: H
authors.lastname: Moon
authors.affiliation: Department of Biological Sciences, School of Life Sciences, Ulsan National Institute of Science and Technology (UNIST), Ulsan, 44919, Korea. sabsab7@unist.ac.kr.
authors.forename: Yoonji
authors.initials: Y
authors.lastname: Bae
authors.affiliation: Department of Biological Sciences, School of Life Sciences, Ulsan National Institute of Science and Technology (UNIST), Ulsan, 44919, Korea. sabsab7@unist.ac.kr.
authors.forename: Hansol
authors.initials: H
authors.lastname: Kim
authors.affiliation: Department of Biological Sciences, School of Life Sciences, Ulsan National Institute of Science and Technology (UNIST), Ulsan, 44919, Korea. sabsab7@unist.ac.kr.
authors.forename: Sebyung
authors.initials: S
authors.lastname: Kang
mesh_terms: D000595:Amino Acid Sequence; D003116:Color; D008164:Luminescent Proteins / Q000378:metabolism; D061848:Optical Imaging / Q000379:methods*; D010455:Peptides / Q000737:chemistry / Q000378:metabolism*
chemical_list: D008164:Luminescent Proteins; D010455:Peptides; C000625572:SpyCatcher peptide; C000625571:SpyTag peptide