title: A comparison of biological characteristics of three strains of Chinese sacbrood virus in Apis cerana.
abstract: We selected and sequenced the entire genomes of three strains of Chinese sacbrood virus (CSBV): LNQY-2008 (isolated in Qingyuan, Liaoning Province), SXYL-2015 (isolated in Yulin, Shanxi Province), and JLCBS-2014 (isolated in Changbaishan, Jilin Province), by VP1 amino acid (aa) analysis. These strains are endemic in China and infect Apis cerana. Nucleotide sequences, deduced amino acid sequences, genetic backgrounds, and other molecular biological characteristics were analysed. We also examined sensitivity of these virus strains to temperature, pH, and organic solvents, as well as to other physicochemical properties. On the basis of these observations, we compared pathogenicity and tested cross-immunogenicity and protective immunity, using antisera raised against each of the three strains. Our results showed that compared with SXYL-2015, LNQY-2008 has a 10-aa deletion and 3-aa deletion (positions 282-291 and 299-301, respectively), whereas JLCBS-2014 has a 17-aa deletion (positions 284-300). However, the three strains showed no obvious differences in physicochemical properties or pathogenicity. Moreover, there was immune cross-reactivity among the antisera raised against the different strains, implying good protective effects of such antisera. The present study should significantly advance the understanding of the pathogenesis of Chinese sacbrood disease, and offers insights into comprehensive prevention and treatment of, as well as possible protection from, the disease by means of an antiserum.
authors.affiliation: Institute of Animal Husbandry and Veterinary, Jinzhou Medical University, Jinzhou, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Hu
authors.affiliation: Institute of Animal Husbandry and Veterinary, Jinzhou Medical University, Jinzhou, China.
authors.forename: Dongliang
authors.initials: D
authors.lastname: Fei
authors.affiliation: Institute of Animal Husbandry and Veterinary, Jinzhou Medical University, Jinzhou, China.
authors.forename: Lili
authors.initials: L
authors.lastname: Jiang
authors.affiliation: Institute of Animal Husbandry and Veterinary, Jinzhou Medical University, Jinzhou, China.
authors.forename: Dong
authors.initials: D
authors.lastname: Wei
authors.affiliation: Institute of Animal Husbandry and Veterinary, Jinzhou Medical University, Jinzhou, China.
authors.forename: Fangbing
authors.initials: F
authors.lastname: Li
authors.affiliation: Honeybee Research Institute, the Chinese Academy of Agricultural Sciences, Beijing, China.
authors.forename: Qingyun
authors.initials: Q
authors.lastname: Diao
authors.affiliation: Institute of Animal Husbandry and Veterinary, Jinzhou Medical University, Jinzhou, China.
authors.forename: Mingxiao
authors.initials: M
authors.lastname: Ma
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001516:Bees / Q000187:drug effects / Q000276:immunology / Q000821:virology*; D002681:China; D016679:Genome, Viral; D006863:Hydrogen-Ion Concentration; D007106:Immune Sera / Q000302:isolation & purification / Q000494:pharmacology; D007814:Larva / Q000187:drug effects / Q000276:immunology / Q000821:virology*; D008807:Mice, Inbred BALB C; D010802:Phylogeny; D012328:RNA Viruses / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification / Q000472:pathogenicity; D016415:Sequence Alignment; D013696:Temperature; D000073336:Whole Genome Sequencing
chemical_list: D007106:Immune Sera