title: Complete genomic characterization of two European-genotype porcine reproductive and respiratory syndrome virus isolates in Fujian province of China.
abstract: Porcine reproductive and respiratory syndrome (PRRS) is considered one of the most devastating swine diseases worldwide, resulting in immense economic losses. PRRS virus (PRRSV) is divided into two major genotypes, European (type 1) and the North American (type 2). Type 1 PRRSV have recently emerged in Fujian province (South China), and this might have a significant impact on the Chinese pig industry. From 2013 to 2014, two type 1 PRRSV strains, named FJEU13 and FJQEU14, were isolated from piglets and sows with respiratory problems and reproductive disorders in Fujian province. The full genome length of the two isolates was 14,869-15,062 nucleotides (nt), excluding the poly(A) tail. These isolates shared 86.0-89.9% sequence identity with the prototypic strains Lelystad virus (LV) and 82.8-92% with Chinese type 1 PRRSV strains, but only 59.9-60.1% with the North American reference strain VR-2332. However, they were 82.9% identical to each other. Nonstructural protein 2 (Nsp2) and ORF3-ORF5 were the most variable regions when compared to other type 1 PRRSV strains. Nsp2 and ORF3 contained multiple discontinuous deletions and a 204-bp deletion in NSP2 in isolate FJQEU14, which has never been described in other Chinese type 1 PRRSV strains. All of these results might be useful for understanding the epidemic status of type 1 PRRSV in China.
authors.affiliation: College of Life Sciences of Longyan University, Longyan, 364012, Fujian, China.|Fujian Provincial Key Laboratory for the Prevention and Control of Animal Infectious Diseases and Biotechnology, Longyan, 364012, Fujian, China.|College of Veterinary Medicine, South China Agricultural University, Guangzhou, 510642, Guangdong, China.
authors.forename: Jian-Kui
authors.initials: JK
authors.lastname: Liu
authors.affiliation: College of Life Sciences of Longyan University, Longyan, 364012, Fujian, China.|Fujian Provincial Key Laboratory for the Prevention and Control of Animal Infectious Diseases and Biotechnology, Longyan, 364012, Fujian, China.
authors.forename: Chun-Hua
authors.initials: CH
authors.lastname: Wei
authors.affiliation: College of Life Sciences of Longyan University, Longyan, 364012, Fujian, China.|Fujian Provincial Key Laboratory for the Prevention and Control of Animal Infectious Diseases and Biotechnology, Longyan, 364012, Fujian, China.
authors.forename: Ai-Ling
authors.initials: AL
authors.lastname: Dai
authors.affiliation: College of Life Sciences of Longyan University, Longyan, 364012, Fujian, China.|Fujian Provincial Key Laboratory for the Prevention and Control of Animal Infectious Diseases and Biotechnology, Longyan, 364012, Fujian, China.
authors.forename: Ke-Wei
authors.initials: KW
authors.lastname: Fan
authors.affiliation: College of Life Sciences of Longyan University, Longyan, 364012, Fujian, China.|Fujian Provincial Key Laboratory for the Prevention and Control of Animal Infectious Diseases and Biotechnology, Longyan, 364012, Fujian, China.
authors.forename: Bing-Hui
authors.initials: BH
authors.lastname: Yang
authors.affiliation: College of Life Sciences of Longyan University, Longyan, 364012, Fujian, China.|Fujian Provincial Key Laboratory for the Prevention and Control of Animal Infectious Diseases and Biotechnology, Longyan, 364012, Fujian, China.
authors.forename: Chun-Fang
authors.initials: CF
authors.lastname: Huang
authors.affiliation: College of Life Sciences of Longyan University, Longyan, 364012, Fujian, China.|Fujian Provincial Key Laboratory for the Prevention and Control of Animal Infectious Diseases and Biotechnology, Longyan, 364012, Fujian, China.
authors.forename: Xiao-Hua
authors.initials: XH
authors.lastname: Li
authors.affiliation: College of Life Sciences of Longyan University, Longyan, 364012, Fujian, China. lyyxy1988@126.com.|Fujian Provincial Key Laboratory for the Prevention and Control of Animal Infectious Diseases and Biotechnology, Longyan, 364012, Fujian, China. lyyxy1988@126.com.
authors.forename: Xiao-Yan
authors.initials: XY
authors.lastname: Yang
authors.affiliation: College of Veterinary Medicine, South China Agricultural University, Guangzhou, 510642, Guangdong, China.
authors.forename: Man-Lin
authors.initials: ML
authors.lastname: Luo
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002681:China; D014644:Genetic Variation; D016679:Genome, Viral; D023281:Genomics; D005838:Genotype; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010802:Phylogeny; D019318:Porcine Reproductive and Respiratory Syndrome / Q000821:virology*; D019316:Porcine respiratory and reproductive syndrome virus / Q000737:chemistry / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D012367:RNA, Viral / Q000235:genetics; D016415:Sequence Alignment; D013552:Swine; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics
chemical_list: D012367:RNA, Viral; D014764:Viral Proteins
keywords: Nsp2; ORF5; genetic variation; porcine reproductive and respiratory syndrome virus (PRRSV); type 1