title: Detection of Protein Interactions in T3S Systems Using Yeast Two-Hybrid Analysis.
abstract: Two-hybrid systems, sometimes termed interaction traps, are genetic systems designed to find and analyze interactions between proteins. The most common systems are yeast based (commonly Saccharomyces cerevisae) and rely on the functional reconstitution of the GAL4 transcriptional activator. Reporter genes, such as the lacZ gene of Escherichia coli (encodes β-galactosidase), are placed under GAL4-dependent transcriptional control to provide quick and reliable detection of protein interactions. In this method the use of a yeast-based two-hybrid system is described to study protein interactions between components of type III secretion systems.
authors.affiliation: Department of Biomedical Sciences, School of Medicine and Health Sciences, University of North Dakota, Grand Forks, ND, USA. matthew.nilles@med.und.edu.
authors.forename: Matthew L
authors.initials: ML
authors.lastname: Nilles
mesh_terms: D001426:Bacterial Proteins / Q000378:metabolism*; D015870:Gene Expression; D017930:Genes, Reporter; D011485:Protein Binding; D025941:Protein Interaction Mapping / Q000379:methods*; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism; D014170:Transformation, Genetic; D020798:Two-Hybrid System Techniques; D000069296:Type III Secretion Systems / Q000378:metabolism*; D001616:beta-Galactosidase / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D000069296:Type III Secretion Systems; D001616:beta-Galactosidase
keywords: Protein–protein interactions; Two-hybrid; Type III secretion system