title: The fluctuating ribosome: thermal molecular dynamics characterized by neutron scattering.
abstract: Conformational changes associated with ribosome function have been identified by X-ray crystallography and cryo-electron microscopy. These methods, however, inform poorly on timescales. Neutron scattering is well adapted for direct measurements of thermal molecular dynamics, the 'lubricant' for the conformational fluctuations required for biological activity. The method was applied to compare water dynamics and conformational fluctuations in the 30 S and 50 S ribosomal subunits from Haloarcula marismortui, under high salt, stable conditions. Similar free and hydration water diffusion parameters are found for both subunits. With respect to the 50 S subunit, the 30 S is characterized by a softer force constant and larger mean square displacements (MSD), which would facilitate conformational adjustments required for messenger and transfer RNA binding. It has been shown previously that systems from mesophiles and extremophiles are adapted to have similar MSD under their respective physiological conditions. This suggests that the results presented are not specific to halophiles in high salt but a general property of ribosome dynamics under corresponding, active conditions. The current study opens new perspectives for neutron scattering characterization of component functional molecular dynamics within the ribosome.
authors.affiliation: Institut Laue Langevin, F-38042 Grenoble, France.|Institut de Biologie Structurale (IBS), Univ. Grenoble Alpes, CEA, CNRS, 38044 Grenoble, France.
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Zaccai
authors.affiliation: Institut Laue Langevin, F-38042 Grenoble, France.|CNR-IOM, OGG, F-38042 Grenoble, France.
authors.forename: Francesca
authors.initials: F
authors.lastname: Natali
authors.affiliation: Institut Laue Langevin, F-38042 Grenoble, France.|Univ. Grenoble Alpes, LiPhy, F-38044 Grenoble, France.
authors.forename: Judith
authors.initials: J
authors.lastname: Peters
authors.affiliation: Institut de Systématique, Evolution, Biodiversité, ISYEB - UMR 7205- CNRS, MNHN, UPMC, EPHE UPMC, Sorbonne Universités, 57 rue Cuvier, CP 50, 75005 Paris, France.|Institute of Physics, Charles University, Faculty of Mathematics and Physics, CZ-121 16 Prague, Czech Republic.
authors.forename: Martina
authors.initials: M
authors.lastname: Řihová
authors.affiliation: Weizmann Institute, Department of Structural Biology, 76100 Rehovot, Israel.
authors.forename: Ella
authors.initials: E
authors.lastname: Zimmerman
authors.affiliation: Institut Laue Langevin, F-38042 Grenoble, France.
authors.forename: J
authors.initials: J
authors.lastname: Ollivier
authors.affiliation: Institut Laue Langevin, F-38042 Grenoble, France.|Institut Charles Sadron, CNRS-UdS, 67034 Strasbourg Cedex 2, France.
authors.forename: J
authors.initials: J
authors.lastname: Combet
authors.affiliation: Institut de Systématique, Evolution, Biodiversité, ISYEB - UMR 7205- CNRS, MNHN, UPMC, EPHE UPMC, Sorbonne Universités, 57 rue Cuvier, CP 50, 75005 Paris, France.
authors.forename: Marie-Christine
authors.initials: MC
authors.lastname: Maurel
authors.affiliation: Weizmann Institute, Department of Structural Biology, 76100 Rehovot, Israel.
authors.forename: Anat
authors.initials: A
authors.lastname: Bashan
authors.affiliation: Weizmann Institute, Department of Structural Biology, 76100 Rehovot, Israel.
authors.forename: Ada
authors.initials: A
authors.lastname: Yonath
mesh_terms: D019614:Haloarcula marismortui / Q000737:chemistry*; D056004:Molecular Dynamics Simulation; D033363:Neutron Diffraction; D019642:RNA, Archaeal / Q000737:chemistry*; D012333:RNA, Messenger / Q000737:chemistry*; D054748:Ribosome Subunits, Large, Archaeal / Q000737:chemistry*; D054749:Ribosome Subunits, Small, Archaeal / Q000737:chemistry*
chemical_list: D019642:RNA, Archaeal; D012333:RNA, Messenger