title: Prosthetic Valve Endocarditis Caused byBartonella henselae: A Case Report of Molecular Diagnostics Informing Nonsurgical Management.
abstract: Identifying the pathogen responsible for culture-negative valve endocarditis often depends on molecular studies performed on surgical specimens. A patient with Ehlers-Danlos syndrome who had an aortic graft, a mechanical aortic valve, and a mitral anulloplasty ring presented with culture-negative prosthetic valve endocarditis and aortic graft infection. Research-based polymerase chain reaction (PCR)/electrospray ionization mass spectrometry on peripheral blood samples identifiedBartonella henselae. Quantitative PCR targeting the16S-23S ribonucleic acid intergenic region and Western immunoblotting confirmed this result. This, in turn, permitted early initiation of pathogen-directed therapy and subsequent successful medical management ofB henselaeprosthetic valve endocarditis and aortic graft infection.
authors.affiliation: Division of Infectious Diseases and HIV Medicine, Department of Medicine, and.
authors.forename: Patricia
authors.initials: P
authors.lastname: Bartley
authors.affiliation: Aix-Marseille Université , Marseille , France.
authors.forename: Emmanouil
authors.initials: E
authors.lastname: Angelakis
authors.affiliation: Aix-Marseille Université , Marseille , France.
authors.forename: Didier
authors.initials: D
authors.lastname: Raoult
authors.affiliation: Ibis Biosciences, Inc. , Carlsbad , California.
authors.forename: Rangarajan
authors.initials: R
authors.lastname: Sampath
authors.affiliation: Division of Infectious Diseases and HIV Medicine, Department of Medicine, and; Departments of Pathology, Pharmacology, and Molecular Biology and Microbiology and Biochemistry, Case Western Reserve University School of Medicine, Cleveland, Ohio;; Geriatric Research Education and Clinical Center, Louis Stokes Cleveland Department of Veterans Affairs Medical Center, Cleveland, Ohio.
authors.forename: Robert A
authors.initials: RA
authors.lastname: Bonomo
authors.affiliation: Division of Infectious Diseases and HIV Medicine, Department of Medicine, and; Geriatric Research Education and Clinical Center, Louis Stokes Cleveland Department of Veterans Affairs Medical Center, Cleveland, Ohio.
authors.forename: Robin L P
authors.initials: RL
authors.lastname: Jump
keywords: Bartonella henselae; Ehlers-Danlos syndrome; culture-negative endocarditis; electrospray ionization mass spectrometry; quantitative PCR.