title: Induction of Laccase, Lignin Peroxidase and Manganese Peroxidase Activities in White-Rot Fungi Using Copper Complexes.
abstract: Ligninolytic enzymes, such as laccase, lignin peroxidase and manganese peroxidase, are biotechnologically-important enzymes. The ability of five white-rot fungal strainsDaedaleopsis confragosa,Fomes fomentarius,Trametes gibbosa,Trametes suaveolensandTrametes versicolorto produce these enzymes has been studied. Three different copper(II) complexes have been prepared ((Him)[Cu(im)₄(H₂O)₂](btc)·3H₂O, where im = imidazole, H₃btc = 1,3,5-benzenetricarboxylic acid, [Cu₃(pmdien)₃(btc)](ClO₄)₃·6H₂O) and [Cu₃(mdpta)₃(btc)](ClO₄)₃·4H₂O, where pmdien =N,N,N',N'',N''-pentamethyl-diethylenetriamine and mdpta =N,N-bis-(3-aminopropyl)methyl- amine), and their potential application for laccase and peroxidases induction have been tested. The enzyme-inducing activities of the complexes were compared with that of copper sulfate, and it has been found that all of the complexes are suitable for the induction of laccase and peroxidase activities in white-rot fungi; however, the newly-synthesized complex M1 showed the greatest potential for the induction. With respect to the different copper inducers, this parameter seems to be important for enzyme activity, which depends also on the fungal strains.
authors.affiliation: Department of Chemistry and Biochemistry, Mendel University in Brno, Zemedelska 1, CZ-613 00 Brno, Czech Republic. mxtinka@seznam.cz.
authors.forename: Martina
authors.initials: M
authors.lastname: Vrsanska
authors.affiliation: Department of Chemistry and Biochemistry, Mendel University in Brno, Zemedelska 1, CZ-613 00 Brno, Czech Republic. stanislava.voberkova@mendelu.cz.|Central European Institute of Technology, Brno University of Technology, Purkynova 123, CZ-612 00 Brno, Czech Republic. stanislava.voberkova@mendelu.cz.
authors.forename: Stanislava
authors.initials: S
authors.lastname: Voberkova
authors.affiliation: Environmental Inorganic Chemistry, Department of Chemical and Biological Engineering, Chalmers University of Technology, SE-412 96 Göteborg, Sweden. langer@chalmers.se.
authors.forename: Vratislav
authors.initials: V
authors.lastname: Langer
authors.affiliation: Department of Forest Protection and Wildlife Management, Faculty of Forestry and Wood Technology, Mendel University in Brno, Zemedelska 1, CZ-613 00 Brno, Czech Republic. palovcik@mendelu.cz.
authors.forename: Dagmar
authors.initials: D
authors.lastname: Palovcikova
authors.affiliation: Department of Chemistry and Biochemistry, Mendel University in Brno, Zemedelska 1, CZ-613 00 Brno, Czech Republic. amitavamoulick@gmail.com.|Central European Institute of Technology, Brno University of Technology, Purkynova 123, CZ-612 00 Brno, Czech Republic. amitavamoulick@gmail.com.
authors.forename: Amitava
authors.initials: A
authors.lastname: Moulick
authors.affiliation: Department of Chemistry and Biochemistry, Mendel University in Brno, Zemedelska 1, CZ-613 00 Brno, Czech Republic. vojtech.adam@mendelu.cz.|Central European Institute of Technology, Brno University of Technology, Purkynova 123, CZ-612 00 Brno, Czech Republic. vojtech.adam@mendelu.cz.
authors.forename: Vojtech
authors.initials: V
authors.lastname: Adam
authors.affiliation: Department of Chemistry and Biochemistry, Mendel University in Brno, Zemedelska 1, CZ-613 00 Brno, Czech Republic. paulko@centrum.cz.|Central European Institute of Technology, Brno University of Technology, Purkynova 123, CZ-612 00 Brno, Czech Republic. paulko@centrum.cz.
authors.forename: Pavel
authors.initials: P
authors.lastname: Kopel
mesh_terms: D056831:Coordination Complexes / Q000737:chemistry / Q000494:pharmacology*; D003300:Copper / Q000737:chemistry; D004790:Enzyme Induction; D005656:Fungal Proteins / Q000096:biosynthesis / Q000235:genetics*; D015971:Gene Expression Regulation, Enzymologic; D006860:Hydrogen Bonding; D042845:Laccase / Q000096:biosynthesis / Q000235:genetics*; D010544:Peroxidases / Q000096:biosynthesis / Q000235:genetics*; D055454:Trametes / Q000201:enzymology*
chemical_list: D056831:Coordination Complexes; D005656:Fungal Proteins; D003300:Copper; D042845:Laccase; D010544:Peroxidases; C042858:lignin peroxidase; C051129:manganese peroxidase
keywords: copper complex; enzymatic induction; laccase; peroxidase; trimesic acid