title: Anti-fatigue activity of polysaccharide fractions from Lepidium meyenii Walp. (maca).
abstract: The two fractions of polysaccharide MPS-1 and MPS-2 were extracted from Lepidium meyenii Walp. (maca) by water, and purified using a DEAE-52 and a Sephadex G-100 column. The molecular weight (MW) of MPS-1 was 7.6kDa, and the MWof MPS-2 was 6.7kDa. The MPS-1 was composed of xylose, arabinose, galactose and glucose, with the mole ratio 1:1.7:3.3:30.5; the MPS-2 was composed of arabinose, galactose and glucose, with the mole ratio 1:1.3:36.8. The IR spectrum implied that only α-pyranose existed in MPS-1, and both α-pyranose and β-pyranose existed in MPS-2. The anti-fatigue activities of MPS-1 and MPS-2 were measured by the forced swimming test, along with the determination of blood lactate (BLA), urea nitrogen (BUN), lactic dehydrogenase (LDH) activity and liver glycogen (LG). The results indicated that both MPS-1 and MPS-2 presented dose-dependently positive effects on the fatigue related parameters. Additionally, MPS-2 has a better anti-fatigue effect than MPS-1.
authors.affiliation: State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, China; School of Food Science and Technology, Jiangnan University, Wuxi 214122, China; National Engineering Research Center for Functional Food, Jiangnan University, Wuxi 214122, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Li
authors.affiliation: School of Food Science and Technology, Jiangnan University, Wuxi 214122, China; National Engineering Research Center for Functional Food, Jiangnan University, Wuxi 214122, China.
authors.forename: Qingrui
authors.initials: Q
authors.lastname: Sun
authors.affiliation: School of Food Science and Technology, Jiangnan University, Wuxi 214122, China; National Engineering Research Center for Functional Food, Jiangnan University, Wuxi 214122, China.
authors.forename: Qingran
authors.initials: Q
authors.lastname: Meng
authors.affiliation: School of Food Science and Technology, Jiangnan University, Wuxi 214122, China; National Engineering Research Center for Functional Food, Jiangnan University, Wuxi 214122, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Wang
authors.affiliation: The Second Military Medical University, 200433 Shanghai, China.
authors.forename: Wentao
authors.initials: W
authors.lastname: Xiong
authors.affiliation: State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, China; School of Food Science and Technology, Jiangnan University, Wuxi 214122, China; National Engineering Research Center for Functional Food, Jiangnan University, Wuxi 214122, China. Electronic address: lianfu@jiangnan.edu.cn.
authors.forename: Lianfu
authors.initials: L
authors.lastname: Zhang
mesh_terms: D000818:Animals; D001089:Arabinose / Q000032:analysis; D001806:Blood Urea Nitrogen; D005591:Chemical Fractionation / Q000379:methods; D004040:Dietary Carbohydrates / Q000302:isolation & purification / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004734:Energy Metabolism / Q000187:drug effects*; D005221:Fatigue / Q000188:drug therapy* / Q000378:metabolism / Q000503:physiopathology; D005690:Galactose / Q000032:analysis; D005947:Glucose / Q000032:analysis; D006003:Glycogen / Q000378:metabolism; D006868:Hydrolysis; D007770:L-Lactate Dehydrogenase / Q000097:blood; D019344:Lactic Acid / Q000097:blood; D029686:Lepidium / Q000737:chemistry*; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008297:Male; D051379:Mice; D008970:Molecular Weight; D010936:Plant Extracts / Q000737:chemistry; D011134:Polysaccharides / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D013550:Swimming; D014994:Xylose / Q000032:analysis
chemical_list: D004040:Dietary Carbohydrates; D010936:Plant Extracts; D011134:Polysaccharides; D019344:Lactic Acid; D006003:Glycogen; D014994:Xylose; D001089:Arabinose; D007770:L-Lactate Dehydrogenase; D005947:Glucose; D005690:Galactose
keywords: Anti-fatigue; Maca (Lepidium meyenii Walp.); Polysaccharide