title: Structure Studies of the Extracellular Polysaccharide from Trichoderma sp. KK19L1 and Its Antitumor Effect via Cell Cycle Arrest and Apoptosis.
abstract: An extracellular polysaccharide TP1A was purified from the fermented broth of Trichoderma sp. KK19L1 by combination of Q Sepharose fast flow and Sephacryl S-300 chromatography. TP1A was composed of Man, Gal, and Glc in a molar ratio of about 3.0:5.1:8.1. The molar mass of TP1A was about 40.0 kDa. Methylation and NMR analysis indicated that the probable structure of TP1A was [→4,6)-α-D-Glcp(1→6)-β-D-Galf(1→6)-β-D-Galf(1→2,6)-β-D-Galf(1→2,6)-β-D-Galf(1→2,6)-β-D-Galf(1→2,6)-α-D-Manp(1→2,6)-α-D-Manp(1→] with [α-D-Glcp(1→] and [α-D-Manp(1→6)-α-D-Glcp(1→6)-α-D-Glcp(1→] as branches. The antitumor study showed that TP1A was able to inhibit the cell viability of HeLa and MCF-7 cells. TP1A could arrest HeLa cells in G2/M phase and induce HeLa cell apoptosis. These findings suggest that fungal polysaccharides could be a potential source for antitumor agents.
authors.affiliation: Key Laboratory of Medicinal Chemistry and Molecular Diagnosis, Ministry of Education, Baoding, China. lihongyan1447@163.com.|College of Chemistry and Environment Science, Hebei University, Baoding, China. lihongyan1447@163.com.
authors.forename: Hongyan
authors.initials: H
authors.lastname: Li
authors.affiliation: Key Laboratory of Medicinal Chemistry and Molecular Diagnosis, Ministry of Education, Baoding, China.|College of Chemistry and Environment Science, Hebei University, Baoding, China.
authors.forename: He
authors.initials: H
authors.lastname: Yu
authors.affiliation: Key Laboratory of Medicinal Chemistry and Molecular Diagnosis, Ministry of Education, Baoding, China. hjzhu@hbu.edu.cn.|College of Pharmaceutical Science, Hebei University, Baoding, China. hjzhu@hbu.edu.cn.
authors.forename: Huajie
authors.initials: H
authors.lastname: Zhu
mesh_terms: D000970:Antineoplastic Agents / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects*; D002240:Carbohydrate Sequence; D062610:Fungal Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology; D059565:G2 Phase Cell Cycle Checkpoints / Q000187:drug effects*; D005690:Galactose / Q000737:chemistry; D005947:Glucose / Q000737:chemistry; D006367:HeLa Cells; D006801:Humans; D061986:MCF-7 Cells; D008358:Mannose / Q000737:chemistry; D014242:Trichoderma / Q000737:chemistry* / Q000378:metabolism
chemical_list: D000970:Antineoplastic Agents; D062610:Fungal Polysaccharides; D005947:Glucose; D008358:Mannose; D005690:Galactose
keywords: Antitumor activity; Extracellular polysaccharide; Structure; Trichoderma sp. KK19L1