title: High-Resolution Cell-Type Specific Analysis of Cytokinins in Sorted Root Cell Populations of Arabidopsis thaliana.
abstract: We describe a method combining fluorescence-activated cell sorting (FACS) with one-step miniaturized isolation and accurate quantification of cytokinins (CKs) using ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) to measure these phytohormones in specific cell types of Arabidopsis thaliana roots. The methodology provides information of unprecedented resolution about spatial distributions of CKs, and thus should facilitate attempts to elucidate regulatory networks involved in root developmental processes.
authors.affiliation: Laboratory of Growth Regulators, Centre of the Region Haná for Biotechnological and Agricultural Research, Institute of Experimental Botany AS CR & Faculty of Science, Palacký University, Šlechtitelů 27, CZ-78371, Olomouc, Czech Republic. novako@ueb.cas.cz.
authors.forename: Ondřej
authors.initials: O
authors.lastname: Novák
authors.affiliation: Department of Forest Genetics and Plant Physiology, Umeå Plant Science Centre, Swedish University of Agricultural Sciences, SE-901 83, Umeå, Sweden.
authors.forename: Ioanna
authors.initials: I
authors.lastname: Antoniadi
authors.affiliation: Department of Forest Genetics and Plant Physiology, Umeå Plant Science Centre, Swedish University of Agricultural Sciences, SE-901 83, Umeå, Sweden.
authors.forename: Karin
authors.initials: K
authors.lastname: Ljung
mesh_terms: D017360:Arabidopsis / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003583:Cytokinins / Q000378:metabolism*; D005434:Flow Cytometry / Q000379:methods; D053263:Gene Regulatory Networks / Q000502:physiology; D010937:Plant Growth Regulators / Q000378:metabolism*; D018517:Plant Roots / Q000378:metabolism*; D053719:Tandem Mass Spectrometry / Q000379:methods
chemical_list: D029681:Arabidopsis Proteins; D003583:Cytokinins; D010937:Plant Growth Regulators
keywords: Arabidopsis; Cytokinins; Fluorescence-activated cell sorting (FACS); Liquid chromatography–mass spectrometry (LC-MS); Protoplasts; Roots; Solid-phase extraction (SPE)