title: Challenges and Opportunities in Linking Long Noncoding RNAs to Cardiovascular, Lung, and Blood Diseases.
abstract: The new millennium heralds an unanticipated surge of genomic information, most notably an expansive class of long noncoding RNAs (lncRNAs). These transcripts, which now outnumber all protein-coding genes, often exhibit the same characteristics as mRNAs (RNA polymerase II-dependent, 5' methyl-capped, multiexonic, polyadenylated); yet, they do not encode for stable, well-conserved proteins. Elucidating the function of all relevant lncRNAs in heart, vasculature, lung, and blood is essential for generating a complete interactome in these tissues. This is particularly evident because an increasing number of investigators perform RNA-sequencing experiments where, typically, annotated lncRNAs exhibit impressive changes in gene expression. How does one go about evaluating an lncRNA when the sequence of the transcript lends no insight into how it may function within a cell type? Here, we provide a brief overview for the rational study of lncRNAs.
authors.affiliation: From the Memorial Heart and Vascular Center, University of Massachusetts Medical School, Worcester (J.E.F.); and Aab Cardiovascular Research Institute, University of Rochester School of Medicine and Dentistry, NY (J.M.M.).
authors.forename: Jane E
authors.initials: JE
authors.lastname: Freedman
authors.affiliation: From the Memorial Heart and Vascular Center, University of Massachusetts Medical School, Worcester (J.E.F.); and Aab Cardiovascular Research Institute, University of Rochester School of Medicine and Dentistry, NY (J.M.M.). j.m.miano@rochester.edu.
authors.forename: Joseph M
authors.initials: JM
authors.lastname: Miano
mesh_terms: D000818:Animals; D002318:Cardiovascular Diseases / Q000235:genetics* / Q000378:metabolism; D019295:Computational Biology; D005786:Gene Expression Regulation; D056726:Genetic Association Studies; D005819:Genetic Markers; D020022:Genetic Predisposition to Disease; D023281:Genomics / Q000379:methods*; D006402:Hematologic Diseases / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D008171:Lung Diseases / Q000235:genetics* / Q000378:metabolism; D010641:Phenotype; D062085:RNA, Long Noncoding / Q000235:genetics* / Q000378:metabolism
chemical_list: D005819:Genetic Markers; D062085:RNA, Long Noncoding
keywords: CRISPR; disease; genomics; method; noncoding RNA