title: Hypoxia-induced vasculogenic mimicry formation in human colorectal cancer cells: Involvement of HIF-1a, Claudin-4, and E-cadherin and Vimentin.
abstract: Vasculogenic mimicry (VM) plays an important role in colorectal cancer (CRC) metastasis, and both hypoxia and the epithelial-mesenchymal transition (EMT) are necessary for VM. In this study, HIF-1α expression was upregulated in the VM-positive CRC cell line HCT-116 and thereby affected the expression of the EMT-related markers Claudin-4, E-cadherin (E-cd) and Vimentin(VIM). SB431542 and U0126EtOH, which can inhibit of EMT were used to treat HCT-116 and HCT-8 in these experiments. Both of the inhibitors had significant effect on EMT markers and the formations of VM in CRC cells. In addition, knockdown of HIF-1α in the HCT-116 cells inhibited their capacity for VM. Our study reveals a regulatory role for HIF-1α in VM and suggests that targeting either HIF-1α or EMT may be a valuable strategy for the elimination of CRC metastasis.
authors.affiliation: Oncology Department of Shanghai Municipal Hospital of Traditional Chinese Medicine affiliated to Shanghai TCM University, Shanghai, 200071, China.
authors.forename: Wen
authors.initials: W
authors.lastname: Li
authors.affiliation: Oncology Department of Shanghai Municipal Hospital of Traditional Chinese Medicine affiliated to Shanghai TCM University, Shanghai, 200071, China.
authors.forename: ShaoQi
authors.initials: S
authors.lastname: Zong
authors.affiliation: Oncology Department of Shanghai Municipal Hospital of Traditional Chinese Medicine affiliated to Shanghai TCM University, Shanghai, 200071, China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Shi
authors.affiliation: Oncology Department of Shanghai Municipal Hospital of Traditional Chinese Medicine affiliated to Shanghai TCM University, Shanghai, 200071, China.
authors.forename: HongJia
authors.initials: H
authors.lastname: Li
authors.affiliation: Mental diseases Department of Shanghai Municipal Hospital of Traditional Chinese Medicine affiliated to Shanghai TCM University, Shanghai, 200071, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Xu
authors.affiliation: Oncology Department of Shanghai Municipal Hospital of Traditional Chinese Medicine affiliated to Shanghai TCM University, Shanghai, 200071, China.
authors.forename: Fenggang
authors.initials: F
authors.lastname: Hou
mesh_terms: D000818:Animals; D015703:Antigens, CD; D015820:Cadherins / Q000378:metabolism*; D015687:Cell Hypoxia; D002465:Cell Movement; D062506:Claudin-4 / Q000378:metabolism*; D015179:Colorectal Neoplasms / Q000098:blood supply* / Q000378:metabolism* / Q000473:pathology; D058750:Epithelial-Mesenchymal Transition / Q000235:genetics; D015972:Gene Expression Regulation, Neoplastic; D045325:HCT116 Cells; D019073:HT29 Cells; D006801:Humans; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000378:metabolism*; D008297:Male; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009361:Neoplasm Invasiveness; D009389:Neovascularization, Pathologic / Q000378:metabolism*; D014746:Vimentin / Q000378:metabolism*; D023041:Xenograft Model Antitumor Assays
chemical_list: D015703:Antigens, CD; C513384:CDH1 protein, human; D015820:Cadherins; D062506:Claudin-4; D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D014746:Vimentin