title: Near-infrared ratiometric fluorescent detection of arginine in lysosome with a new hemicyanine derivative.
abstract: A Near-Infrared (NIR) ratiometric and on-off fluorescent probe 1 was synthesized, which exhibited high sensitivity and excellent selectivity in arginine (Arg) recognition. 1 can quantitatively detect Arg in concentration range from 0 to 150μM with the LOD of 138nM. Further, it displayed excellent selectivity and anti-interference over many biological species, metal ions and anions. Due to its ability to target lysosome, the response of Arg to this probe in a living cell was successfully tracked in a ratiometric manner via fluorescence imaging.
authors.affiliation: College of Chemistry and Molecular Engineering, Zhengzhou University, Zhengzhou 450001, China. Electronic address: yumm@zzu.edu.cn.
authors.forename: Mingming
authors.initials: M
authors.lastname: Yu
authors.affiliation: College of Chemistry and Molecular Engineering, Zhengzhou University, Zhengzhou 450001, China.
authors.forename: Weiwei
authors.initials: W
authors.lastname: Du
authors.affiliation: College of Chemistry and Molecular Engineering, Zhengzhou University, Zhengzhou 450001, China.
authors.forename: Haixia
authors.initials: H
authors.lastname: Li
authors.affiliation: Key Laboratory of Photochemical Conversion and Optoelectronic Materials, Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing, 100190, China. Electronic address: zhanghongyan@mail.ipc.ac.cn.
authors.forename: Hongyan
authors.initials: H
authors.lastname: Zhang
authors.affiliation: College of Chemistry and Molecular Engineering, Zhengzhou University, Zhengzhou 450001, China. Electronic address: lizx@zzu.edu.cn.
authors.forename: Zhanxian
authors.initials: Z
authors.lastname: Li
mesh_terms: D001120:Arginine / Q000032:analysis*; D015374:Biosensing Techniques / Q000379:methods; D002232:Carbocyanines / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D008247:Lysosomes / Q000737:chemistry*; D018613:Microscopy, Confocal / Q000379:methods; D061848:Optical Imaging / Q000379:methods*
chemical_list: D002232:Carbocyanines; D005456:Fluorescent Dyes; C000601156:tetramethylene hemicyanine; D001120:Arginine
keywords: Arg; Cell imaging; Lysosome; Near-infrared; Ratiometric; Recognition