title: Anti-obesity effect of radix Angelica sinensis and candidate causative genes in transcriptome analyses of adipose tissues in high-fat diet-induced mice.
abstract: We have previously reported that radix Angelica sinensis (RAS) suppressed body weight and altered the expression of the fat mass and obesity associated (FTO) gene in mice with high fat diet (HFD)-induced obesity. In the present study we performed RNA sequencing-mediated transcriptome analysis to elucidate the molecular mechanisms underlying the anti-obesogenic effects of RAS in mice. The results revealed that 36 differentially-expressed genes (DEGs) were identified in adipose tissues from the RAS supplementation group (DH) and control group (HC). These 36 DEGs were clustered into 297 functional gene ontology (GO) categories, among which several GO annotations and signaling pathways were associated with lipid homeostasis. Six out of the 36 DEGs were identified to be involved in lipid metabolism, with the APOA2 gene a potential anti-obesogenic influence. The expression pattern revealed by RNA-Seq was identical to the results of quantitative real-time PCR (qPCR). Therefore, RAS supplementation in HFD-induced obese mice was associated with an anti-obesogenic global transcriptomic response. This study provides insight into potential applications of RAS in obesity therapy.
authors.affiliation: Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Zhong
authors.affiliation: Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, China.
authors.forename: Hao
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, China.
authors.forename: Xiaoyue
authors.initials: X
authors.lastname: Duan
authors.affiliation: Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, China.
authors.forename: Jiangtao
authors.initials: J
authors.lastname: Hu
authors.affiliation: Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, China.
authors.forename: Linjie
authors.initials: L
authors.lastname: Wang
authors.affiliation: Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, China.
authors.forename: Li
authors.initials: L
authors.lastname: Li
authors.affiliation: Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, China.
authors.forename: Hongping
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, China. Electronic address: niulili@sicau.edu.cn.
authors.forename: Lili
authors.initials: L
authors.lastname: Niu
mesh_terms: D000273:Adipose Tissue / Q000187:drug effects* / Q000378:metabolism*; D029971:Angelica sinensis; D000818:Animals; D019440:Anti-Obesity Agents / Q000627:therapeutic use*; D059305:Diet, High-Fat / Q000009:adverse effects; D004365:Drugs, Chinese Herbal / Q000627:therapeutic use*; D005260:Female; D020869:Gene Expression Profiling; D053263:Gene Regulatory Networks / Q000187:drug effects; D050356:Lipid Metabolism / Q000187:drug effects / Q000235:genetics; D051379:Mice; D009765:Obesity / Q000188:drug therapy* / Q000209:etiology / Q000235:genetics*; D008517:Phytotherapy; D012333:RNA, Messenger / Q000235:genetics; D015398:Signal Transduction / Q000187:drug effects / Q000235:genetics
chemical_list: D019440:Anti-Obesity Agents; D004365:Drugs, Chinese Herbal; D012333:RNA, Messenger; C044613:angelicae sinensis extract
keywords: Adipose; Mice; Obesity; Radix Angelica sinensis; Transcriptome