title: A whole genome gene content phylogenetic analysis of anopheline mosquitoes.
abstract: Construction of stringent gene content matrices was accomplished for 21 Anopheline mosquito species and strains and four outgroups species. The presence absence matrix using e-75as a cutoff in single linkage clustering had over 17,000 ortholog groups. We used the gene content matrix to generate a phylogenetic hypothesis that is in general agreement with gene sequence based phylogenies. In addition to establishing a congruent gene content phylogeny we examined the consistency of three methods for analyzing presence absence data - unweighted parsimony, dollo parsimonly and maximum likelihood using a BINGAMMA model. An examination of the chromosomal location of the gains and losses in the presence absence matrix revealed a low frequency of gains and losses at centromeres and tips of chromosomes.
authors.affiliation: Sackler Institute for Comparative Genomics, American Museum of Natural History, New York, NY 10024, USA; Cancer Institute of New Jersey, Rutgers University, New Brunswick, NJ, USA.
authors.forename: Jeffrey A
authors.initials: JA
authors.lastname: Rosenfeld
authors.affiliation: Sackler Institute for Comparative Genomics, American Museum of Natural History, New York, NY 10024, USA.
authors.forename: Sara
authors.initials: S
authors.lastname: Oppenheim
authors.affiliation: Sackler Institute for Comparative Genomics, American Museum of Natural History, New York, NY 10024, USA. Electronic address: desalle@amnh.org.
authors.forename: Rob
authors.initials: R
authors.lastname: DeSalle
mesh_terms: D000818:Animals; D000852:Anopheles / Q000235:genetics*; D059006:Chromosomes, Insect / Q000235:genetics; D049750:Genome, Insect; D059785:Karyotype; D010802:Phylogeny
keywords: Anopheline; Chromosome; Dollo parsimony; Gene content; Phylogeny; e-value