title: Production of ethanol from lignocellulosics: an enzymatic venture.
abstract: The major objective of the present investigation was to evaluate the effect of enzymatic pretreatment onLantana camarafor improved yield of reducing sugar and bioethanol production. An optimum enzymatic delignification (88.79 %) was achieved after 8 h of incubation. After delignification the substrate was further treated with the mixture of carbohydratases for appropriate saccharification. The enzyme treated substrate yielded maximum reducing sugar (713.33 mg/g dry substrate) after 9 h of saccharification. Monosaccharide content in the saccharified samples were quantified using high performance liquid chromatography (HPLC) system. Using conventional yeast strain, 9.63 g/L bioethanol was produced from saccharified samples ofLantana camara. Structural changes ofLantana camarabefore and after enzymatic pretreatment were further investigated through Fourier transformed infrared spectroscopy (FTIR), X-ray diffraction (XRD) and Scanning electron microscopy (SEM).
authors.affiliation: Microbial Biotechnology and Downstream Processing Laboratory, Agricultural and Food Engineering Department, Indian Institute of Technology, Kharagpur, India 721302.
authors.forename: Arindam
authors.initials: A
authors.lastname: Kuila
authors.affiliation: Microbial Biotechnology and Downstream Processing Laboratory, Agricultural and Food Engineering Department, Indian Institute of Technology, Kharagpur, India 721302.
authors.forename: Mainak
authors.initials: M
authors.lastname: Mukhopadhyay
authors.affiliation: Indian Oil Corporation Ltd., R & D Centre, Faridabad, India 121 007.
authors.forename: D K
authors.initials: DK
authors.lastname: Tuli
authors.affiliation: Microbial Biotechnology and Downstream Processing Laboratory, Agricultural and Food Engineering Department, Indian Institute of Technology, Kharagpur, India 721302.
authors.forename: Rintu
authors.initials: R
authors.lastname: Banerjee
keywords: Lantana camara; X-ray diffraction; bioethanol; delignification; optimization; saccharification