title: CRISPR-Cas9 System as a Versatile Tool for Genome Engineering in Human Cells.
abstract: Targeted nucleases are influential instruments for intervening in genome revision with great accuracy. RNA-guided Cas9 nucleases produced from clustered regularly interspaced short palindromic repeats (CRISPR)-Cas systems have noticeably altered the means to modify the genomes of distinct organisms. They can be notably used to facilitate effective genome manipulation in eukaryotic cells by clearly detailing a 20-nt targeting sequence inside its directed RNA. We discuss the most recent advancements in the molecular basis of the type II CRISPR/Cas system and encapsulate applications and elements affecting its use in human cells. We also propose possible applications covering its uses ranging from basic science to implementation in the clinic.
authors.affiliation: Department of Gynecology and Obstetrics, Zhongshan Hospital of Xiamen University, Xiamen, China.|Department of Gynecology and Obstetrics, Qilu Hospital of Shandong University, Jinan, China.
authors.forename: Xuelian
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Gynecology and Obstetrics, Zhongshan Hospital of Xiamen University, Xiamen, China.
authors.forename: Xiumin
authors.initials: X
authors.lastname: Huang
authors.affiliation: Department of Gynecology and Obstetrics, Zhongshan Hospital of Xiamen University, Xiamen, China.
authors.forename: Xiuli
authors.initials: X
authors.lastname: Fang
authors.affiliation: Department of Gynecology and Obstetrics, Qilu Hospital of Shandong University, Jinan, China.
authors.forename: Youzhong
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Marine Biogenetic Resources, the Third Institute of Oceanography, State of Oceanic Administration, Xiamen, China.
authors.forename: Wanpeng
authors.initials: W
authors.lastname: Wang