title: Mediator-free interaction of glucose oxidase, as model enzyme for immobilization, with Al-doped and undoped ZnO thin films laser-deposited on polycarbonate supports.
abstract: Al doped and undoped ZnO thin films were deposited by pulsed-laser deposition on polycarbonate sheets. The films were characterized by optical transmission, Hall effect measurement, XRD and SEM. Optical transmission and surface reflectometry studies showed good transparency with thicknesses ∼100nm and surface roughness of 10nm. Hall effect measurements showed that the sheet carrier concentration was -1.44×1015cm-2for AZO and -6×1014cm-2for ZnO. The films were then modified by drop-casting glucose oxidase (GOx) without the use of any mediators. Higher protein concentration was observed on ZnO as compared to AZO with higher specific activity for ZnO (0.042Umg-1) compared to AZO (0.032Umg-1), and was in agreement with cyclic voltemmetry (CV). X-ray photoelectron spectroscopy (XPS) suggested that the protein was bound by dipole interactions between AZO lattice oxygen and the amino group of the enzyme. Chronoamperometry showed sensitivity of 5.5μAmM-1cm-2towards glucose for GOx/AZO and 2.2μAmM-1cm-2for GOx/ZnO. The limit of detection (LoD) was 167μM of glucose for GOx/AZO, as compared to 360μM for GOx/ZnO. The linearity was 0.28-28mM for GOx/AZO whereas it was 0.6-28mM for GOx/ZnO with a response time of 10s. Possibly due to higher enzyme loading, the decrease of impedance in presence of glucose was larger for GOx/ZnO as compared to GOx/AZO in electrochemical impedance spectroscopy (EIS). Analyses with clinical blood serum samples showed that the systems had good reproducibility and accuracy. The characteristics of novel ZnO and AZO thin films with GOx as a model enzyme, should prove useful for the future fabrication of inexpensive, highly sensitive, disposable electrochemical biosensors for high throughput diagnostics.
authors.affiliation: Bhupat and Jyoti Mehta School of Biosciences, Department of Biotechnology, Indian Institute of Technology, Madras, Chennai-600036, India.
authors.forename: Fidal
authors.initials: F
authors.lastname: V T K P
authors.affiliation: School of Physical Sciences and National Centre for Plasma Science and Technology, Dublin City University, Collins Avenue, Dublin9, Ireland.
authors.forename: Saikumar
authors.initials: S
authors.lastname: Inguva
authors.affiliation: School of Physical Sciences and National Centre for Plasma Science and Technology, Dublin City University, Collins Avenue, Dublin9, Ireland.
authors.forename: Satheesh
authors.initials: S
authors.lastname: Krishnamurthy
authors.affiliation: School of Biotechnology, Dublin City University, Collins Avenue, Dublin9, Ireland.
authors.forename: Enrico
authors.initials: E
authors.lastname: Marsili
authors.affiliation: School of Physical Sciences and National Centre for Plasma Science and Technology, Dublin City University, Collins Avenue, Dublin9, Ireland.
authors.forename: Jean-Paul
authors.initials: JP
authors.lastname: Mosnier
authors.affiliation: Bhupat and Jyoti Mehta School of Biosciences, Department of Biotechnology, Indian Institute of Technology, Madras, Chennai-600036, India. Electronic address: chandrasainathan@gmail.com.
authors.forename: Chandra
authors.initials: C
authors.lastname: T S
mesh_terms: D000535:Aluminum / Q000737:chemistry; D015374:Biosensing Techniques / Q000379:methods*; D001709:Biotechnology; D001774:Blood Chemical Analysis / Q000379:methods; D001786:Blood Glucose / Q000032:analysis; D058266:Dielectric Spectroscopy; D004800:Enzymes, Immobilized / Q000737:chemistry* / Q000378:metabolism; D005949:Glucose Oxidase / Q000737:chemistry* / Q000378:metabolism; D006801:Humans; D007700:Kinetics; D007834:Lasers; D011076:Polycarboxylate Cement; D015034:Zinc Oxide / Q000737:chemistry*
chemical_list: D001786:Blood Glucose; D004800:Enzymes, Immobilized; D011076:Polycarboxylate Cement; C005506:polycarbonate; D000535:Aluminum; D005949:Glucose Oxidase; D015034:Zinc Oxide
keywords: Al-doped ZnO; Glucose oxidase; Polycarbonate; ZnO