title: Promoter hypermethylation ofKLOTHO; an anti-senescence related gene in colorectal cancer patients of Kashmir valley.
abstract: Hypermethylation of CpG islands located in the promoter regions of genes is a major event in the development of the majority of cancer types, due to the subsequent aberrant silencing of important tumor suppressor genes.KLOTHO; a novel gene associated primarily with suppressing senescence has been shown to contribute to tumorigenesis as a result of its impaired function. Recently the relevance ofKLOTHOpromoter hypermethylation in colorectal carcinoma in humans has been reported. We analyzed the promoter hypermethylation ofKLOTHOgene in 50 histopathologically confirmed tumor and adjacent normal tissues of colorectal cancer patients. Methylation was assessed by bisulfite conversion of DNA followed by methylation specific-polymerase chain reaction. Methylation status was compared with gender, smoking status and histopathological parameters of patients. Promoter hypermethylation inKLOTHOgene was detected in 86% (43/50) of tumor tissues and 14% (7/50) of adjacent normal tissues. The methylation pattern differed significantly between tumor and adjacent normal tissues (P<0.0001). However, no association was found between promoter hypermethylation status and gender (P=0.68), smoking status (P=0.64) or other histopathological parameters (P>0.05) of colorectal cancer patients. We conclude that this novel tumor suppressor gene is epigenetically inactivated in colorectal cancer in our population paving way towards the potential ofKLOTHOpromoter hypermethylation as a predictor of the prognosis in colorectal cancer patients.
authors.affiliation: Department of General and Minimal Access Surgery, SKIMS Srinagar, India.
authors.forename: Malik
authors.initials: M
authors.lastname: Perveez
authors.affiliation: Department of General and Minimal Access Surgery, SKIMS Srinagar, India.
authors.forename: Malik
authors.initials: M
authors.lastname: Ajaz
authors.affiliation: Department of Immunology and Molecular Medicine, SKIMS Srinagar, India.
authors.forename: Dil
authors.initials: D
authors.lastname: Afroze
keywords: Colorectal cancer; KLOTHO; Promoter hypermethylation; Tumor suppressor