title: Histone Purification from Saccharomyces cerevisiae.
abstract: The nucleosome structure consists of a histone octamer made by a tetramer of H3-H4 histones and two dimers of H2A-H2B. Nucleosomes undergo extensive posttranslational modifications that regulate nucleosome interactions, position, and stability.We describe a protocol allowing the robust purification of histones from the yeast Saccharomyces cerevisiae. This method appears to be suitable to quantitatively analyze specific posttranslational histone modifications.
authors.affiliation: Cancer Research Center of Marseille (CRCM), U1068 Inserm, UMR7258 CNRS, Aix Marseille University (AMU), Institut Paoli-Calmettes, Marseille, 13009, France.
authors.forename: Frederic
authors.initials: F
authors.lastname: Jourquin
authors.affiliation: Cancer Research Center of Marseille (CRCM), U1068 Inserm, UMR7258 CNRS, Aix Marseille University (AMU), Institut Paoli-Calmettes, Marseille, 13009, France. vincent.geli@inserm.fr.
authors.forename: Vincent
authors.initials: V
authors.lastname: Géli
mesh_terms: D006657:Histones / Q000302:isolation & purification* / Q000378:metabolism; D009707:Nucleosomes / Q000378:metabolism; D011499:Protein Processing, Post-Translational; D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D004718:Saccharomycetales / Q000378:metabolism
chemical_list: D006657:Histones; D009707:Nucleosomes
keywords: Budding yeast; Chromatin; Histones; Purification