title: Megakaryocytes from CYCS mutation-associated thrombocytopenia release platelets by both proplatelet-dependent and -independent processes.
abstract: Thrombocytopenia Cargeeg is a rare autosomal dominant disorder and one of three thrombocytopenias caused by mutation of cytochrome c (Online Mendelian Inheritance in Man entry THC4). Our previous observations of platelet-like structures in the marrow space and early platelet production in vitro suggested that the low platelet phenotype in Thrombocytopenia Cargeeg subjects is caused by premature release of platelets into non-vascular regions of the bone marrow. We now show that two processes of platelet release occur in Thrombocytopenia Cargeeg subjects. Circulating platelets have a normal marginal microtubule coil, and cultured megakaryocytes derived from peripheral blood cells of Thrombocytopenia Cargeeg subjects form proplatelets normally and release platelets containing a marginal microtubule coil, consistent with effective platelet release via the proplatelet mechanism. In contrast, platelet-like structures within the extravascular bone marrow space have the dimensions of platelets but lack the marginal microtubule coil, suggesting abnormal proplatelet-independent platelet release. The mechanism of extravascular platelet release remains unclear. The failure to recapitulate this mechanism in vitro implies that the phenotype is not simply an intrinsic property of CYCS mutation-carrying megakaryocytes, but is dependent on the interaction between these cells and their environment.
authors.affiliation: Department of Biochemistry, Otago School of Medical Sciences, University of Otago, Dunedin, New Zealand.
authors.forename: Lily
authors.initials: L
authors.lastname: Ong
authors.affiliation: Department of Pathology, Dunedin School of Medicine, University of Otago, Dunedin, New Zealand.
authors.forename: Ian M
authors.initials: IM
authors.lastname: Morison
authors.affiliation: Department of Biochemistry, Otago School of Medical Sciences, University of Otago, Dunedin, New Zealand.
authors.forename: Elizabeth C
authors.identifier: http://orcid.org/0000-0002-4956-6043
authors.initials: EC
authors.lastname: Ledgerwood
mesh_terms: D001792:Blood Platelets / Q000473:pathology*; D001853:Bone Marrow / Q000473:pathology; D002478:Cells, Cultured; D045304:Cytochromes c / Q000235:genetics*; D006801:Humans; D008533:Megakaryocytes / Q000473:pathology*; D008870:Microtubules; D009154:Mutation; D013921:Thrombocytopenia / Q000235:genetics / Q000473:pathology*
chemical_list: D045304:Cytochromes c
keywords: CYCS-related thrombocytopenia; cytochrome c; human bone marrow; megakaryopoiesis; thrombopoiesis