title: Potential effect of mechano growth factor E-domain peptide on axonal guidance growth in primary cultured cortical neurons of rats.
abstract: Establishing appropriate synaptic connections and plasticity is a critical need in neuronal regeneration and development. Mechano growth factor (MGF) and its C-terminal E-domain peptide with 24 amino acids, MGF-Ct24E, are potential neuroprotective agents. Our preliminary study indicates that Netrin-1 can guide axonal growth and its expression is sensitive to MGF, but how MGF regulates the expression of Netrin-1 and its receptor DCC is still unclear. Here, we investigate the effect of MGF-Ct24E on the expression of Netrin-1 and DCC in primary cultured cortical neurons in vitro and the adult rat brain in vivo. MTT assay shows that MGF-Ct24E can significantly protect primary cortical neurons against nerve injury. There is a significant increase in axonal elongation after MGF-Ct24E treatment at concentrations of 0.5 and 1.0 μg/ml. Real-time polymerase chain reaction assay indicates that MGF-Ct24E can effectively promote the expression of Netrin-1 and DCC in primary cultured cortical neurons. To identify the certain mechanism of MGF-Ct24E on neuronal guidance and growth, adult rats are subjected to intramuscular injection of MGF-Ct24E after traumatic brain injury. Rats injected with MGF-Ct24E start eating and drinking within 14 days, indicating that MGF-Ct24E can promote rehabilitation. HE staining and immunohistochemistry assays of brain section slices reveal that MGF-Ct24E treatment can significantly inhibit the haemorrhage of traumatic brain injury and promote expression of Netrin-1. Further investigation of protein expression by Western blot assay shows that MGF-Ct24E promotes expression of Netrin-1 and DCC after nerve injury. MGF-Ct24E can effectively improve axonal guidance through upregulation of Netrin-1/DCC signalling in neuronal regeneration. Copyright © 2016 John Wiley & Sons, Ltd.
authors.affiliation: Key Laboratory for Biomechanics and Mechanobiology of Ministry of Education, School of Biological Science and Medical Engineering, Beihang University, Beijing, 100191, China.
authors.forename: Meili
authors.initials: M
authors.lastname: Liu
authors.affiliation: Key Laboratory for Biomechanics and Mechanobiology of Ministry of Education, School of Biological Science and Medical Engineering, Beihang University, Beijing, 100191, China.
authors.forename: Xufeng
authors.initials: X
authors.lastname: Niu
authors.affiliation: Key Laboratory for Biomechanics and Mechanobiology of Ministry of Education, School of Biological Science and Medical Engineering, Beihang University, Beijing, 100191, China.
authors.forename: Gang
authors.initials: G
authors.lastname: Zhou
authors.affiliation: Key Laboratory for Biomechanics and Mechanobiology of Ministry of Education, School of Biological Science and Medical Engineering, Beihang University, Beijing, 100191, China.
authors.forename: Zhengtai
authors.initials: Z
authors.lastname: Jia
authors.affiliation: Key Laboratory for Biomechanics and Mechanobiology of Ministry of Education, School of Biological Science and Medical Engineering, Beihang University, Beijing, 100191, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Li
authors.affiliation: Key Laboratory for Biomechanics and Mechanobiology of Ministry of Education, School of Biological Science and Medical Engineering, Beihang University, Beijing, 100191, China.|National Research Center for Rehabilitation Technical Aids, Beijing, 100176, China.
authors.forename: Yubo
authors.initials: Y
authors.lastname: Fan
mesh_terms: D000375:Aging; D000818:Animals; D000071437:Axon Guidance / Q000187:drug effects*; D000070642:Brain Injuries, Traumatic / Q000473:pathology; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000166:cytology*; D000089622:Doublecortin Domain Proteins; D007334:Insulin-Like Growth Factor I / Q000494:pharmacology*; D007382:Intermediate Filaments / Q000378:metabolism; D008869:Microtubule-Associated Proteins / Q000378:metabolism; D009417:Nerve Tissue / Q000187:drug effects / Q000293:injuries / Q000473:pathology; D000075388:Netrin-1 / Q000378:metabolism; D009474:Neurons / Q000187:drug effects / Q000378:metabolism*; D009479:Neuropeptides / Q000378:metabolism; D010455:Peptides / Q000494:pharmacology*; D020011:Protective Agents / Q000494:pharmacology; D017207:Rats, Sprague-Dawley
chemical_list: D000089622:Doublecortin Domain Proteins; D008869:Microtubule-Associated Proteins; D009479:Neuropeptides; D010455:Peptides; D020011:Protective Agents; D000075388:Netrin-1; D007334:Insulin-Like Growth Factor I
keywords: DCC; Netrin-1; axonal guidance growth; mechano growth factor; nerve regeneration