title: NK cell degranulation as a marker for measuring antibody-dependent cytotoxicity in neutralizing and non-neutralizing human sera from dengue patients.
abstract: The study assessed antibody-dependent NK cell degranulation, a biomarker relevant to antibody-dependent cell cytotoxicity (ADCC), to analyze dengue immune sera. We first determined binding intensity of patient sera to the surface of DENV-infected cells and examined the types of antigens expressed on infected cells. Antigens from pre-membrane (PreM) and envelope (E), but not from NS proteins were detected on the surface of infected cells. After adding NK cells to infected target cells previously treated with patient sera, rapid NK cell degranulation was observed. Non-neutralizing patient sera generated comparable NK cell degranulation as that of neutralizing sera, suggesting ADCC may be a protective mechanism apart from Ab neutralization. The level of NK cell degranulation varied dramatically among human individuals and was associated with the level of CD16 expression on NK cells, informing on the complexity of ADCC among human population.
authors.affiliation: Henry Jackson Foundation, United States. Electronic address: peifang.sun2.ctr@mail.mil.
authors.forename: Peifang
authors.initials: P
authors.lastname: Sun
authors.affiliation: Naval Medical Research Center, United States.
authors.forename: Brian J
authors.initials: BJ
authors.lastname: Morrison
authors.affiliation: Navy Bloodborne Infection Management Center, United States.
authors.forename: Charmagne G
authors.initials: CG
authors.lastname: Beckett
authors.affiliation: Henry Jackson Foundation, United States.
authors.forename: Zhaodong
authors.initials: Z
authors.lastname: Liang
authors.affiliation: Henry Jackson Foundation, United States.
authors.forename: Nishith
authors.initials: N
authors.lastname: Nagabhushana
authors.affiliation: University of Maryland College Park, United States.
authors.forename: An
authors.initials: A
authors.lastname: Li
authors.affiliation: Naval Medical Research Center, United States.
authors.forename: Kevin R
authors.initials: KR
authors.lastname: Porter
authors.affiliation: Naval Medical Research Center, United States.
authors.forename: Maya
authors.initials: M
authors.lastname: Williams
mesh_terms: D057134:Antibodies, Neutralizing / Q000097:blood / Q000276:immunology*; D000914:Antibodies, Viral / Q000097:blood / Q000276:immunology; D000920:Antibody-Dependent Cell Cytotoxicity; D015550:Cell Degranulation; D003715:Dengue / Q000276:immunology*; D003716:Dengue Virus / Q000276:immunology*; D058851:GPI-Linked Proteins / Q000235:genetics / Q000276:immunology; D006801:Humans; D007694:Killer Cells, Natural / Q000276:immunology*; D017452:Receptors, IgG / Q000235:genetics / Q000276:immunology
chemical_list: D057134:Antibodies, Neutralizing; D000914:Antibodies, Viral; C428200:FCGR3B protein, human; D058851:GPI-Linked Proteins; D017452:Receptors, IgG
keywords: ADCC; CD16; Dengue serum; Dengue virus; NK cell degranulation