title: CD8+T Cells from Human Neonates Are Biased toward an Innate Immune Response.
abstract: To better understand why human neonates show a poor response to intracellular pathogens, we compared gene expression and histone modification profiles of neonatal naive CD8+T cells with that of their adult counterparts. We found that neonatal lymphocytes have a distinct epigenomic landscape associated with a lower expression of genes involved in T cell receptor (TCR) signaling and cytotoxicity and a higher expression of genes involved in the cell cycle and innate immunity. Functional studies corroborated that neonatal CD8+T cells are less cytotoxic, transcribe antimicrobial peptides, and produce reactive oxygen species. Altogether, our results show that neonatal CD8+T cells have a specific genetic program biased toward the innate immune response. These findings will contribute to better diagnosis and management of the neonatal immune response.
authors.affiliation: Centro de Investigación en Dinámica Celular (IICBA), Universidad Autónoma del Estado de Morelos, Av. Universidad 1001, Chamilpa, Cuernavaca Morelos 62100, Mexico; INSERM, TAGC UMR_S 1090, 13288 Marseille Cedex 09, France; Aix-Marseille University, TAGC UMR_S 1090, 13288 Marseille Cedex 09, France.
authors.forename: Ariel O
authors.initials: AO
authors.lastname: Galindo-Albarrán
authors.affiliation: Centro de Investigación en Dinámica Celular (IICBA), Universidad Autónoma del Estado de Morelos, Av. Universidad 1001, Chamilpa, Cuernavaca Morelos 62100, Mexico.
authors.forename: Oscar H
authors.initials: OH
authors.lastname: López-Portales
authors.affiliation: Centro de Investigación en Dinámica Celular (IICBA), Universidad Autónoma del Estado de Morelos, Av. Universidad 1001, Chamilpa, Cuernavaca Morelos 62100, Mexico.
authors.forename: Darely Y
authors.initials: DY
authors.lastname: Gutiérrez-Reyna
authors.affiliation: Centro de Investigación en Dinámica Celular (IICBA), Universidad Autónoma del Estado de Morelos, Av. Universidad 1001, Chamilpa, Cuernavaca Morelos 62100, Mexico.
authors.forename: Otoniel
authors.initials: O
authors.lastname: Rodríguez-Jorge
authors.affiliation: Centro de Investigación en Dinámica Celular (IICBA), Universidad Autónoma del Estado de Morelos, Av. Universidad 1001, Chamilpa, Cuernavaca Morelos 62100, Mexico.
authors.forename: José Antonio
authors.initials: JA
authors.lastname: Sánchez-Villanueva
authors.affiliation: Centro de Investigación en Dinámica Celular (IICBA), Universidad Autónoma del Estado de Morelos, Av. Universidad 1001, Chamilpa, Cuernavaca Morelos 62100, Mexico.
authors.forename: Oscar
authors.initials: O
authors.lastname: Ramírez-Pliego
authors.affiliation: INSERM, TAGC UMR_S 1090, 13288 Marseille Cedex 09, France; Aix-Marseille University, TAGC UMR_S 1090, 13288 Marseille Cedex 09, France; Transcriptomique et Génomique de Marseille-Luminy (TGML), IBiSA platform, 13288 Marseille, France.
authors.forename: Aurélie
authors.initials: A
authors.lastname: Bergon
authors.affiliation: INSERM, TAGC UMR_S 1090, 13288 Marseille Cedex 09, France; Aix-Marseille University, TAGC UMR_S 1090, 13288 Marseille Cedex 09, France; Transcriptomique et Génomique de Marseille-Luminy (TGML), IBiSA platform, 13288 Marseille, France.
authors.forename: Béatrice
authors.initials: B
authors.lastname: Loriod
authors.affiliation: INSERM, TAGC UMR_S 1090, 13288 Marseille Cedex 09, France; Aix-Marseille University, TAGC UMR_S 1090, 13288 Marseille Cedex 09, France; Transcriptomique et Génomique de Marseille-Luminy (TGML), IBiSA platform, 13288 Marseille, France.
authors.forename: Hélène
authors.initials: H
authors.lastname: Holota
authors.affiliation: INSERM, TAGC UMR_S 1090, 13288 Marseille Cedex 09, France; Aix-Marseille University, TAGC UMR_S 1090, 13288 Marseille Cedex 09, France; Transcriptomique et Génomique de Marseille-Luminy (TGML), IBiSA platform, 13288 Marseille, France.
authors.forename: Jean
authors.initials: J
authors.lastname: Imbert
authors.affiliation: Centro de Investigación en Dinámica Celular (IICBA), Universidad Autónoma del Estado de Morelos, Av. Universidad 1001, Chamilpa, Cuernavaca Morelos 62100, Mexico.
authors.forename: Armando
authors.initials: A
authors.lastname: Hernández-Mendoza
authors.affiliation: Centre d'Immunologie de Marseille-Luminy, Aix Marseille Université, INSERM, Centre National de la Recherche Scientifique (CNRS), 13288 Marseille Cedex 09, France.
authors.forename: Pierre
authors.initials: P
authors.lastname: Ferrier
authors.affiliation: Structural Biology and BioComputing Program, Spanish National Cancer Research Center, CNIO, 28029 Madrid, Spain.
authors.forename: Enrique
authors.initials: E
authors.lastname: Carrillo-de Santa Pau
authors.affiliation: Structural Biology and BioComputing Program, Spanish National Cancer Research Center, CNIO, 28029 Madrid, Spain.
authors.forename: Alfonso
authors.initials: A
authors.lastname: Valencia
authors.affiliation: INSERM, TAGC UMR_S 1090, 13288 Marseille Cedex 09, France; Aix-Marseille University, TAGC UMR_S 1090, 13288 Marseille Cedex 09, France. Electronic address: salvatore.spicuglia@inserm.fr.
authors.forename: Salvatore
authors.initials: S
authors.lastname: Spicuglia
authors.affiliation: Centro de Investigación en Dinámica Celular (IICBA), Universidad Autónoma del Estado de Morelos, Av. Universidad 1001, Chamilpa, Cuernavaca Morelos 62100, Mexico. Electronic address: santana@uaem.mx.
authors.forename: M Angélica
authors.initials: MA
authors.lastname: Santana
mesh_terms: D000328:Adult; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D003602:Cytotoxicity, Immunologic / Q000235:genetics; D044127:Epigenesis, Genetic; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D006801:Humans; D007113:Immunity, Innate / Q000235:genetics / Q000276:immunology*; D007231:Infant, Newborn; D014157:Transcription Factors / Q000378:metabolism
chemical_list: D014157:Transcription Factors
keywords: T lymphocyte; epigenome; human immunity; immune response