title: Complete genome sequence of Novosphingobium resinovorum SA1, a versatile xenobiotic-degrading bacterium capable of utilizing sulfanilic acid.
abstract: Sulfanilic acid (4-aminobenzenesulfonic acid) is a sulfonated aromatic amine widely used in chemical industries for synthesis of various organic dyes and sulfa drugs. There are quite a few microbial co-cultures or single isolates capable of completely degrading this compound. Novosphingobium resinovorum SA1 was the first single bacterium which could utilize sulfanilic acid as its sole carbon, nitrogen and sulfur source. The strain has versatile catabolic routes for the bioconversion of numerous other aromatic compounds. Here, the complete genome sequence of the N. resinovorum SA1 strain is reported. The genome consists of a circular chromosome of 3.8 Mbp and four extrachromosomal elements between 67 and 1 759.8 kbp in size. Three alternative 3-ketoadipate pathways were identified on the plasmids. Sulfanilic acid is decomposed via a modified 3-ketoadipate pathway and the oxygenases involved form a phylogenetically separate branch on the tree. Sequence analysis of these elements might provide a genetic background for deeper insight into the versatile catabolic metabolism of various aromatic xenobiotics, including sulfanilic acid and its derivatives. Moreover, this is also a good model strain for understanding the role and evolution of multiple genetic elements within a single strain.
authors.affiliation: Department of Biotechnology, University of Szeged, Közép fasor 52, H-6726 Szeged, Hungary; Institute of Biophysics, Biological Research Center, Temesvári krt. 62, H-6726 Szeged, Hungary.
authors.forename: Botond
authors.initials: B
authors.lastname: Hegedűs
authors.affiliation: Department of Biotechnology, University of Szeged, Közép fasor 52, H-6726 Szeged, Hungary; Institute of Plant Biology, Biological Research Center, Temesvári krt. 62, H-6726 Szeged, Hungary.
authors.forename: Péter B
authors.initials: PB
authors.lastname: Kós
authors.affiliation: Seqomics Ltd, Vállalkozók útja 7, H-6782 Mórahalom, Hungary.
authors.forename: Balázs
authors.initials: B
authors.lastname: Bálint
authors.affiliation: Seqomics Ltd, Vállalkozók útja 7, H-6782 Mórahalom, Hungary.
authors.forename: Gergely
authors.initials: G
authors.lastname: Maróti
authors.affiliation: School of Science, Monash University Malaysia, Jalan Lagoon Selatan, Bandar Sunway, 47500 Petaling Jaya, Selangor, Malaysia.
authors.forename: Han Ming
authors.initials: HM
authors.lastname: Gan
authors.affiliation: Department of Biotechnology, University of Szeged, Közép fasor 52, H-6726 Szeged, Hungary.
authors.forename: Katalin
authors.initials: K
authors.lastname: Perei
authors.affiliation: Department of Biotechnology, University of Szeged, Közép fasor 52, H-6726 Szeged, Hungary; Institute of Biophysics, Biological Research Center, Temesvári krt. 62, H-6726 Szeged, Hungary; Institute of Environmental and Technological Sciences, Közép fasor 52, H-6726 Szeged, Hungary. Electronic address: rakhely.gabor@brc.mta.hu.
authors.forename: Gábor
authors.initials: G
authors.lastname: Rákhely
mesh_terms: D020561:Alphaproteobacteria / Q000235:genetics* / Q000378:metabolism; D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics; D016680:Genome, Bacterial / Q000235:genetics*; D017422:Sequence Analysis, DNA; D013425:Sulfanilic Acids / Q000032:analysis / Q000378:metabolism*
chemical_list: D004269:DNA, Bacterial; D013425:Sulfanilic Acids
keywords: Aminobenzenesulfonate; Biodegradation of xenobiotics; Megaplasmid; Multiple extrachromosomal elements; Novosphingobium resinovorum SA1 genome