title: Epitope analysis of Japanese cedar pollen allergen Cry j2 with a human IgM class monoclonal antibody 404-117.
abstract: Japanese cedar pollen allergen Cry j2 is a causal allergen of seasonal pollinosis in Japan. To analyze B cell epitopes of Cry j2, we established two human-mouse hybridomas secreting IgM class human monoclonal antibodies to Cry j2. A pin-peptide enzyme-linked immunosorbent assay with synthesized icosa peptides showed that 404-117 monoclonal antibody bound to peptides #11-13 with cry j2 amino acid sequence of 101F-L140. Detailed analysis with octa peptides and alanine substituted peptides indicated that an amino acid sequence of 118FKVD121 was an essential for antibody binding. When K119 (Asn) was substituted with alanine, 404-117 monoclonal antibody did not bind to the alanine substituted peptide. We concluded that the 118FKVD121 sequence might have a very important role in early recognition by Cry j2-specific B cells, which could act as antigen presenting cells.
authors.affiliation: Tamagawa University, College of Agriculture, Machida, Tokyo, Japan.
authors.forename: Seiya
authors.initials: S
authors.lastname: Chiba
authors.affiliation: Tamagawa University, College of Agriculture, Machida, Tokyo, Japan.
authors.forename: Haruka
authors.initials: H
authors.lastname: Yokoyama
authors.affiliation: Tamagawa University, College of Agriculture, Machida, Tokyo, Japan.
authors.forename: Ayane
authors.initials: A
authors.lastname: Kumazawa
authors.affiliation: Tokyo Metropolitan Institute of Medical Science, Setagaya-ku, Tokyo, Japan.
authors.forename: Michie
authors.initials: M
authors.lastname: Shimmoto
authors.affiliation: Tamagawa University, College of Agriculture, Machida, Tokyo, Japan.
authors.forename: Yasunori
authors.initials: Y
authors.lastname: Naganawa
authors.affiliation: Tamagawa University, College of Agriculture, Machida, Tokyo, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Shinmoto
mesh_terms: D000485:Allergens / Q000737:chemistry / Q000276:immunology; D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis*; D000918:Antibody Specificity; D000938:Antigen-Presenting Cells / Q000276:immunology / Q000473:pathology; D052179:Antigens, Plant / Q000737:chemistry / Q000276:immunology; D001402:B-Lymphocytes / Q000276:immunology / Q000473:pathology; D001665:Binding Sites; D032485:Cryptomeria / Q000737:chemistry / Q000276:immunology; D000939:Epitopes / Q000737:chemistry / Q000276:immunology*; D006801:Humans; D006825:Hybridomas / Q000276:immunology / Q000378:metabolism; D007075:Immunoglobulin M / Q000096:biosynthesis*; D007564:Japan; D051379:Mice; D010455:Peptides / Q000737:chemistry / Q000276:immunology; D010940:Plant Proteins / Q000737:chemistry / Q000276:immunology*; D011058:Pollen / Q000737:chemistry / Q000276:immunology*; D011485:Protein Binding; D006255:Rhinitis, Allergic, Seasonal / Q000139:chemically induced / Q000276:immunology* / Q000473:pathology
chemical_list: D000485:Allergens; D000911:Antibodies, Monoclonal; D052179:Antigens, Plant; C065291:Cry j II protein, Cryptomeria japonica; D000939:Epitopes; D007075:Immunoglobulin M; D010455:Peptides; D010940:Plant Proteins
keywords: Human monoclonal antibody; IgM; Japanese cedar pollen; allergen; epitope