title: Cryopreserved Adipose Tissue-Derived Stromal/Stem Cells: Potential for Applications in Clinic and Therapy.
abstract: Adipose-Derived Stromal/Stem Cells (ASC) have considerable potential for regenerative medicine due to their abilities to proliferate, differentiate into multiple cell lineages, high cell yield, relative ease of acquisition, and almost no ethical concerns since they are derived from adult tissue. Storage of ASC by cryopreservation has been well described that maintains high cell yield and viability, stable immunophenotype, and robust differentiation potential post-thaw. This ability is crucial for banking research and for clinical therapeutic purposes that avoid the morbidity related to repetitive liposuction tissue harvests. ASC secrete various biomolecules such as cytokines which are reported to have immunomodulatory properties and therapeutic potential to reverse symptoms of multiple degenerative diseases/disorders. Nevertheless, safety regarding the use of these cells clinically is still under investigation. This chapter focuses on the different aspects of cryopreserved ASC and the methods to evaluate their functionality for future clinical use.
authors.affiliation: Federal University of Sao Paulo, Sao Paulo, SP, Brazil.|Center for Stem Cell Research & Regenerative Medicine, Tulane University, New Orleans, LA, USA.
authors.forename: Fabiana
authors.initials: F
authors.lastname: Zanata
authors.affiliation: Department of Mechanical Engineering, Louisiana State University, Baton Rouge, LA, USA.
authors.forename: Shahensha
authors.initials: S
authors.lastname: Shaik
authors.affiliation: Department of Mechanical Engineering, Louisiana State University, Baton Rouge, LA, USA.
authors.forename: Ram V
authors.initials: RV
authors.lastname: Devireddy
authors.affiliation: La Cell LLC, New Orleans BioInnovation Center, Suite 304, 1441 Canal Street, New Orleans, LA, 70112, USA.
authors.forename: Xiying
authors.initials: X
authors.lastname: Wu
authors.affiliation: Federal University of Sao Paulo, Sao Paulo, SP, Brazil.
authors.forename: Lydia Masako
authors.initials: LM
authors.lastname: Ferreira
authors.affiliation: Center for Stem Cell Research & Regenerative Medicine, Tulane University, New Orleans, LA, USA. jeffrey.gimble@lacell-usa.com.|La Cell LLC, New Orleans BioInnovation Center, Suite 304, 1441 Canal Street, New Orleans, LA, 70112, USA. jeffrey.gimble@lacell-usa.com.
authors.forename: Jeffrey M
authors.initials: JM
authors.lastname: Gimble
mesh_terms: D017667:Adipocytes / Q000166:cytology* / Q000502:physiology; D000273:Adipose Tissue / Q000166:cytology* / Q000502:physiology; D000328:Adult; D018070:Biological Specimen Banks; D001861:Bone Regeneration; D002454:Cell Differentiation; D049109:Cell Proliferation; D002470:Cell Survival / Q000187:drug effects; D064987:Cell- and Tissue-Based Therapy / Q000379:methods*; D015925:Cryopreservation / Q000379:methods*; D003451:Cryoprotective Agents / Q000494:pharmacology; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D019855:Ethylene Glycol / Q000494:pharmacology; D006801:Humans; D016462:Mammaplasty / Q000379:methods; D019636:Neurodegenerative Diseases / Q000628:therapy; D017154:Stromal Cells / Q000166:cytology* / Q000502:physiology / Q000637:transplantation
chemical_list: D003451:Cryoprotective Agents; D019855:Ethylene Glycol; D004121:Dimethyl Sulfoxide
keywords: Adipose tissue; Adipose-derived stromal/stem cells; Cell differentiation; Clinical trials; Cryopreservation; Human cells; Immunophenotype; Regenerative medicine