title: Cytocompatibility testing of cyclodextrin-functionalized antimicrobial textiles-a comprehensive approach.
abstract: Functionalized textiles can be used in wound management to reduce the microbial burden in the wound area, to prevent wound infections, and to avoid cross-contamination between patients. In the present study, a comprehensive in vitro approach to enable the assessment of antibacterial activity of functionalized textiles and cytotoxicity of cyclodextrin (CD)-complexes with chlorhexidine diacetate (CHX), iodine (IOD), and polihexanide (PHMB) is suggested to evaluate their properties for supporting optimal conditions for wound healing. For all β-CD-antiseptic functionalized cotton samples a strong antibacterial effect on the Gram-positive bacteria Staphylococcus aureus and Staphylococcus epidermidis as well as on the Gram-negative bacteria Klebsiella pneumoniae and Escherichia coli was proven. In addition, β-CD-CHX and β-CD-PHMB were effective against the yeast Candida albicans. The growth of Pseudomonas aeruginosa could be reduced significantly by β-CD-IOD and β-CD-PHMB. The established comprehensive testing system for determination of biocompatibility on human HaCaT keratinocytes is suitable for obtaining robust data on cell viability, cytotoxicity and mode of cell death of the β-CD-antiseptic-complexes. The promising results of the high antimicrobial activity of these functionalized textiles show the high potential of such materials in medical applications.
authors.affiliation: Department of Dermatology, University Hospital Jena, Jena, Germany. kirsten.reddersen@med.uni-jena.de.
authors.forename: Kirsten
authors.identifier: http://orcid.org/0000-0001-9814-6419
authors.initials: K
authors.lastname: Reddersen
authors.affiliation: Department of Dermatology, University Hospital Jena, Jena, Germany.
authors.forename: Susanne
authors.initials: S
authors.lastname: Finger
authors.affiliation: Department of Dermatology, University Hospital Jena, Jena, Germany.
authors.forename: Michael
authors.initials: M
authors.lastname: Zieger
authors.affiliation: Department of Dermatology, University Hospital Jena, Jena, Germany.
authors.forename: Cornelia
authors.initials: C
authors.lastname: Wiegand
authors.affiliation: Deutsches Textilforschungszentrum Nord-West e.V. Krefeld, Krefeld, Germany.
authors.forename: Hans-Jürgen
authors.initials: HJ
authors.lastname: Buschmann
authors.affiliation: Department of Dermatology, University Hospital Jena, Jena, Germany.
authors.forename: Peter
authors.initials: P
authors.lastname: Elsner
authors.affiliation: Department of Dermatology, University Hospital Jena, Jena, Germany.
authors.forename: Uta-Christina
authors.initials: UC
authors.lastname: Hipler
mesh_terms: D000890:Anti-Infective Agents / Q000737:chemistry*; D000891:Anti-Infective Agents, Local; D017209:Apoptosis; D001424:Bacterial Infections / Q000188:drug therapy*; D001645:Biguanides; D001672:Biocompatible Materials; D002176:Candida albicans / Q000187:drug effects; D053148:Caspase 3 / Q000378:metabolism; D002460:Cell Line; D002470:Cell Survival; D003505:Cyclodextrins / Q000737:chemistry*; D004926:Escherichia coli / Q000187:drug effects; D005434:Flow Cytometry; D006801:Humans; D015603:Keratinocytes / Q000187:drug effects / Q000382:microbiology; D007711:Klebsiella pneumoniae / Q000187:drug effects; D008422:Materials Testing; D008826:Microbial Sensitivity Tests; D011550:Pseudomonas aeruginosa / Q000187:drug effects; D013203:Staphylococcal Infections / Q000188:drug therapy; D013211:Staphylococcus aureus / Q000187:drug effects; D013212:Staphylococcus epidermidis / Q000187:drug effects; D013784:Textiles
chemical_list: D000890:Anti-Infective Agents; D000891:Anti-Infective Agents, Local; D001645:Biguanides; D001672:Biocompatible Materials; D003505:Cyclodextrins; C031233:polihexanide; C505407:CASP3 protein, human; D053148:Caspase 3