title: Assessment of multifunctional activity of bioactive peptides derived from fermented milk by specific Lactobacillus plantarum strains.
abstract: Milk-derived bioactive peptides with a single activity (e.g., antioxidant, immunomodulatory, or antimicrobial) have been previously well documented; however, few studies describe multifunctional bioactive peptides, which may be preferred over single-activity peptides, as they can simultaneously trigger, modulate, or inhibit multiple physiological pathways. Hence, the aim of this study was to assess the anti-inflammatory, antihemolytic, antioxidant, antimutagenic, and antimicrobial activities of crude extracts (CE) and peptide fractions (<3 and 3-10 kDa) obtained from fermented milks with specific Lactobacillus plantarum strains. Overall, CE showed higher activity than both peptide fractions (<3 and 3-10 kDa) in most of the activities assessed. Furthermore, activity of <3 kDa was generally higher, or at least equal, to the 3 to 10 kDa peptide fractions. In particular, L. plantarum 55 crude extract or their fractions showed the higher anti-inflammatory (723.68-1,759.43μg/mL of diclofenac sodium equivalents), antihemolytic (36.65-74.45% of inhibition), and antioxidant activity [282.8-362.3µmol of Trolox (Sigma-Aldrich, St. Louis, MO) equivalents]. These results provide valuable evidence of multifunctional role of peptides derived of fermented milk by the action of specific L. plantarum strains. Thus, they may be considered for the development of biotechnological products to be used to reduce the risk of disease or to enhance a certain physiological function.
authors.affiliation: Centro de Investigación en Alimentación y Desarrollo A.C. (CIAD), Hermosillo, Sonora 83304, Mexico.
authors.forename: J E
authors.initials: JE
authors.lastname: Aguilar-Toalá
authors.affiliation: Centro de Investigación en Alimentación y Desarrollo A.C. (CIAD), Hermosillo, Sonora 83304, Mexico.
authors.forename: L
authors.initials: L
authors.lastname: Santiago-López
authors.affiliation: Instituto de Tecnologia Química e Biológica, Universidade Nova de Lisboa, P-2780-157 Oeiras, Portugal.
authors.forename: C M
authors.initials: CM
authors.lastname: Peres
authors.affiliation: Instituto Nacional de Inestigação Agraria e Veternária (INIAV), Instituto Português (IP), Estação Agrnómica Nacional (EAN), P-2780-157 Oeiras, Portugal.
authors.forename: C
authors.initials: C
authors.lastname: Peres
authors.affiliation: Unidad de Investigación y Desarrollo en Alimentos (UNIDA), Instituto Tecnológico de Veracruz, Veracruz 91897, Mexico.
authors.forename: H S
authors.initials: HS
authors.lastname: Garcia
authors.affiliation: Centro de Investigación en Alimentación y Desarrollo A.C. (CIAD), Hermosillo, Sonora 83304, Mexico.
authors.forename: B
authors.initials: B
authors.lastname: Vallejo-Cordoba
authors.affiliation: Centro de Investigación en Alimentación y Desarrollo A.C. (CIAD), Hermosillo, Sonora 83304, Mexico.
authors.forename: A F
authors.initials: AF
authors.lastname: González-Córdova
authors.affiliation: Centro de Investigación en Alimentación y Desarrollo A.C. (CIAD), Hermosillo, Sonora 83304, Mexico. Electronic address: ahernandez@ciad.mx.
authors.forename: A
authors.initials: A
authors.lastname: Hernández-Mendoza
mesh_terms: D000818:Animals; D000890:Anti-Infective Agents / Q000032:analysis; D000893:Anti-Inflammatory Agents / Q000032:analysis; D016587:Antimutagenic Agents / Q000032:analysis; D000975:Antioxidants / Q000032:analysis; D043302:Cultured Milk Products / Q000032:analysis*; D005285:Fermentation; D048191:Lactiplantibacillus plantarum / Q000235:genetics / Q000502:physiology*; D008892:Milk / Q000737:chemistry; D008894:Milk Proteins / Q000032:analysis; D010455:Peptides / Q000032:analysis*
chemical_list: D000890:Anti-Infective Agents; D000893:Anti-Inflammatory Agents; D016587:Antimutagenic Agents; D000975:Antioxidants; D008894:Milk Proteins; D010455:Peptides
keywords: Lactobacillus; milk-derived peptide; multifunctional peptides; peptide fraction