title: Experimental Application of Bone Marrow Mesenchymal Stem Cells for the Repair of Intervertebral Disc Annulus Fibrosus.
abstract: BACKGROUND This study provides experimental results on the applicability of bone marrow mesenchymal stem cells (BMSCs) for the repair of intervertebral disc annulus fibrosus in rabbits. MATERIAL AND METHODS Thirty healthy rabbits were randomized into an observation group (n=15) and a control group (n=15). Both groups underwent degeneration of intervertebral disc annulus fibrosus. The observation group was treated with a solution of BMSCs and dexamethasone sodium phosphate, while the control group was treated with dexamethasone sodium phosphate only. RESULTS The two groups were compared for efficacy and pathological conditions after treatment. Both disc height index and level of type II collagen in nucleus pulposus were significantly higher in the observation group than in the control group at 2, 4, 8, and 12 weeks after degeneration (p<0.05 for all comparisons). The percentages of grade 0 and grade 1 were significantly higher in the observation group than in the control group (p<0.05 for both grade 0 and 1 comparisons), while the percentage of grade 4 and grade 5 were significantly lower in the observation group than in the control group (p<0.05 for both grade 4 and 5 comparisons). CONCLUSIONS BMSCs cultured in vitro can effectively repair intervertebral disc annulus fibrosus, which is of positive significance, and thus is clinically recommended.
authors.affiliation: Department of Anatomy, Basic Medical College, Inner Mongolia Medical University, Huhhot, Inner Mongolia, China (mainland).
authors.forename: Xiaohe
authors.initials: X
authors.lastname: Li
authors.affiliation: Department of CT Center, The Second Hospital of Inner Mongolia Medical University, Huhhot, Inner Mongolia, China (mainland).
authors.forename: Yunfeng
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Orthopedics, The First People's Hospital of Wuxue, Wuxue, Hubei, China (mainland).
authors.forename: Bing
authors.initials: B
authors.lastname: Song
authors.affiliation: Department of Anatomy, Basic Medical College, Inner Mongolia Medical University, Huhhot, Inner Mongolia, China (mainland).
authors.forename: He
authors.initials: H
authors.lastname: En
authors.affiliation: Department of Anatomy, Basic Medical College, Inner Mongolia Medical University, Huhhot, Inner Mongolia, China (mainland).
authors.forename: Shang
authors.initials: S
authors.lastname: Gao
authors.affiliation: Department of Anatomy, Basic Medical College, Inner Mongolia Medical University, Huhhot, Inner Mongolia, China (mainland).
authors.forename: Shaojie
authors.initials: S
authors.lastname: Zhang
authors.affiliation: Department of Anatomy, Basic Medical College, Inner Mongolia Medical University, Huhhot, Inner Mongolia, China (mainland).
authors.forename: Yongqiang
authors.initials: Y
authors.lastname: Cai
authors.affiliation: Department of Anatomy, Basic Medical College, Inner Mongolia Medical University, Huhhot, Inner Mongolia, China (mainland).
authors.forename: Zhi-Jun
authors.initials: ZJ
authors.lastname: Li
authors.affiliation: Department of Biochemistry, Basic Medical College, Inner Mongolia Medical University, Huhhot, Inner Mongolia, China (mainland).
authors.forename: Cunbao
authors.initials: C
authors.lastname: Li
authors.affiliation: , Second Affiliated Hospital of Xinxiang Medical College, Huhhot, Inner Mongolia, China (mainland).
authors.forename: Weiping
authors.initials: W
authors.lastname: Wang
authors.affiliation: Department of Anatomy, Basic Medical College, Inner Mongolia Medical University, Huhhot, Inner Mongolia, China (mainland).
authors.forename: Xing
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Anatomy, Basic Medical College, Inner Mongolia Medical University, Huhhot, Inner Mongolia, China (mainland).
authors.forename: Haiyan
authors.initials: H
authors.lastname: Wang
authors.affiliation: The department of anatomy, Basic medical college,Inner Mongolia Medical University, Hohhot, China (mainland).
authors.forename: Zhiqiang
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Department of Anatomy, Basic Medical College, Inner Mongolia Medical University, Huhhot, Inner Mongolia, China (mainland).
authors.forename: Qi
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: Department of Anatomy, Basic Medical College, Inner Mongolia Medical University, Huhhot, Inner Mongolia, China (mainland).
authors.forename: Jierong
authors.initials: J
authors.lastname: Ma
mesh_terms: D000818:Animals; D000070616:Annulus Fibrosus / Q000601:surgery*; D024043:Collagen Type II / Q000032:analysis; D003907:Dexamethasone / Q000031:analogs & derivatives / Q000494:pharmacology; D005260:Female; D007403:Intervertebral Disc / Q000473:pathology; D055959:Intervertebral Disc Degeneration / Q000473:pathology / Q000601:surgery; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation / Q000379:methods*; D059630:Mesenchymal Stem Cells / Q000166:cytology; D023421:Models, Animal; D011817:Rabbits; D023822:Tissue Engineering / Q000379:methods; D014945:Wound Healing
chemical_list: D024043:Collagen Type II; C004180:dexamethasone 21-phosphate; D003907:Dexamethasone