title: All trans retinoic acid depresses the content and activity of the mitochondrial ATP synthase in human keratinocytes.
abstract: Proteomic analysis shows that treatment of keratinocytes cultures with all trans retinoic acid (ATRA), under condition in which it inhibits cell growth, results in marked decrease of the level of the F1-β subunit of the catalytic sector of the mitochondrial FoF1 ATP synthase complex. Enzymatic analysis shows in ATRA-treated keratinocytes a consistent depression of the ATPase activity, with decreased olygomycin sensitivity, indicating an overall alteration of the ATP synthase complex. These findings, together with the previously reported inhibition of respiratory complex I, show that depression of the activity of oxidative phosphorylation enzymes is involved in the cell growth inhibitory action of ATRA.
authors.affiliation: Department of Basic Medical Sciences, Neurosciences and Sense Organs, University 'A. Moro', Bari, Italy.
authors.forename: F
authors.initials: F
authors.lastname: Papa
authors.affiliation: Institute of Biomembranes and Bioenergetics, Italian National Research Council (CNR), Via G. Amendola 165/A, Italy.
authors.forename: R
authors.initials: R
authors.lastname: Lippolis
authors.affiliation: Department of Basic Medical Sciences, Neurosciences and Sense Organs, University 'A. Moro', Bari, Italy.
authors.forename: N
authors.initials: N
authors.lastname: Sardaro
authors.affiliation: Department of Basic Medical Sciences, Neurosciences and Sense Organs, University 'A. Moro', Bari, Italy.
authors.forename: A
authors.initials: A
authors.lastname: Gnoni
authors.affiliation: Department of Basic Medical Sciences, Neurosciences and Sense Organs, University 'A. Moro', Bari, Italy. Electronic address: salvatore.scacco@uniba.it.
authors.forename: S
authors.initials: S
authors.lastname: Scacco
mesh_terms: D049109:Cell Proliferation / Q000187:drug effects / Q000502:physiology*; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D015536:Down-Regulation / Q000187:drug effects / Q000502:physiology; D004789:Enzyme Activation / Q000187:drug effects; D006801:Humans; D015603:Keratinocytes / Q000166:cytology / Q000201:enzymology*; D025261:Mitochondrial Proton-Translocating ATPases / Q000096:biosynthesis*; D014212:Tretinoin / Q000008:administration & dosage*
chemical_list: D014212:Tretinoin; D025261:Mitochondrial Proton-Translocating ATPases
keywords: ATP synthase; All-trans retinoic acid; Human keratinocytes; Mass spectrometry; Proteomics