title: An Ex Vivo Tissue Culture Model for Anti-angiogenic Drug Testing.
abstract: Angiogenesis, defined as the growth of new blood vessels from existing ones, plays a key role in development, growth, and tissue repair. Its necessary role in tumor growth and metastasis has led to the creation of a new category of anti-angiogenic cancer therapies. Preclinical development and evaluation of potential drug candidates require models that mimic real microvascular networks. Here, we describe the rat mesentery culture model as a simple ex vivo assay that offers time-lapse imaging of intact microvascular network remodeling and demonstrate its application for anti-angiogenic drug testing.
authors.affiliation: Department of Biomedical Engineering, Tulane University, New Orleans, LA, USA.
authors.forename: Mohammad S
authors.initials: MS
authors.lastname: Azimi
authors.affiliation: Department of Mathematics, Tulane University, New Orleans, LA, USA.
authors.forename: Michelle
authors.initials: M
authors.lastname: Lacey
authors.affiliation: Department of Pharmacology, Tulane University, New Orleans, LA, USA.
authors.forename: Debasis
authors.initials: D
authors.lastname: Mondal
authors.affiliation: Department of Biomedical Engineering, Tulane University, New Orleans, LA, USA. wmurfee@tulane.edu.
authors.forename: Walter L
authors.initials: WL
authors.lastname: Murfee
mesh_terms: D020533:Angiogenesis Inhibitors / Q000494:pharmacology*; D000818:Animals; D004353:Drug Evaluation, Preclinical; D008643:Mesentery / Q000098:blood supply / Q000166:cytology* / Q000187:drug effects; D055806:Microvessels / Q000187:drug effects / Q000648:ultrastructure*; D008954:Models, Biological; D051381:Rats; D017208:Rats, Wistar; D059008:Time-Lapse Imaging; D046509:Tissue Culture Techniques / Q000379:methods*
chemical_list: D020533:Angiogenesis Inhibitors
keywords: Angiogenesis; Drug screening; Endothelial cell; Mesentery; Microvascular network; Pericytes; Tissue culture