title: [Effect of shift rotation culture on formation and activity of encapsulated hepatocytes aggregates].
abstract: Objective:To observe the effect of uniform and shift rotation culture on the formation and activity of the alginate-chitosan (AC) microencapsulated HepLL immortalized human hepatocytes and HepG2 cells aggregates.Methods:AC microcapsulated HepG2 and HepLL cells were randomly divided into two groups. Each group was divided into 3 subgroups according to uniform and shift rotation culture.The size and number of aggregates were observed and measured under laser confocal microscopy and inverted microscope dynamically. The amount of albumin synthesis was detected by ELISA, the clearance of ammonia was detected by colorimetry, and diazepam conversion function was detected by high performance liquid chromatography (HPLC).Results:On day 6, 8, 10, 12, 14 and 16, the number and size of the aggregates, albumin synthesis, diazepam clearance and ammonium clearance increased significantly in shift rotation culture group than in uniform group (allP<0.01). The albumin synthesis, diazepam clearance, and ammonium clearance in the microencapsulated HepLL groups were significantly higher than those of HepG2 cells at any time (allP<0.01).Conclusion:Shift rotation culture can significantly promote the formation and increase the activity of AC microencapsulated HepLL and HepG2 aggregates, and HepLL cells may be more suitable for bioartificial liver than HepG2.
authors.affiliation: Department of Infectious Diseases, the First Affiliated Hospital, Xiamen University, Xiamen 361000, China;State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, Department of Infectious Disease, the First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310003, China.
authors.forename: Yanshan
authors.initials: Y
authors.lastname: Chen
authors.affiliation: State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, Department of Infectious Disease, the First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310003, China.
authors.forename: Chengbo
authors.initials: C
authors.lastname: Yu
authors.affiliation: State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, Department of Infectious Disease, the First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310003, China.
authors.forename: Hongcui
authors.initials: H
authors.lastname: Cao
authors.affiliation: State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, Department of Infectious Disease, the First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310003, China. ljli@zju.edu.cn.
authors.forename: Lanjuan
authors.initials: L
authors.lastname: Li
mesh_terms: D000418:Albumins / Q000096:biosynthesis / Q000378:metabolism; D000464:Alginates; D000641:Ammonia / Q000378:metabolism; D000818:Animals; D002449:Cell Aggregation / Q000502:physiology*; D018929:Cell Culture Techniques / Q000379:methods*; D002461:Cell Line, Transformed / Q000502:physiology; D048271:Chitosan; D003975:Diazepam / Q000378:metabolism; D020723:Glucuronic Acid; D056945:Hep G2 Cells / Q000166:cytology / Q000502:physiology*; D022781:Hepatocytes / Q000166:cytology / Q000502:physiology*; D006603:Hexuronic Acids; D006801:Humans; D019164:Liver, Artificial; D012399:Rotation
chemical_list: D000418:Albumins; D000464:Alginates; D006603:Hexuronic Acids; D000641:Ammonia; D020723:Glucuronic Acid; D048271:Chitosan; D003975:Diazepam