title: Relationships between the expression of hepatocyte nuclear factors and factors essential for lipoprotein production in a human mesenchymal stem cell line, UE7T-13.
abstract: To clarify the mechanisms regulating lipoprotein production by hepatocyte nuclear factors (HNFs), we generated four kinds of transfectants in human bone marrow mesenchymal stem cells: UE7T-13, stably expressing FOXA2 (also known as HNF3β), HNF4α, HNF1α or co-expressing HNF4α, and HNF1α (HNF4α/HNF1α). In HNF4α/HNF1α transfectants, cellular contents of triglycerides (TG) and cholesterol were markedly higher than in UE7T-13 cells and comparable to those in human hepatoma HepG2 cells. However, TG and cholesterol, which are secreted from cells as components of lipoproteins, were hardly detected in the medium for any of the transfectants. ApoB100 and MTP, which are essential for the formation and secretion of lipoproteins, were undetectable and detected at low levels, respectively, in HNF4α/HNF1α transfectants. We suggest that enforced co-expression of HNF4α and HNF1α is effective for cellular lipid accumulation, while additional factors are probably required for lipoprotein formation and secretion.
authors.affiliation: a Graduate School of Bioresource Sciences , Akita Prefectural University , Akita , Japan.|b Food Processing Research Station , Akita Research Institute of Food and Brewing , Akita , Japan.
authors.forename: Akira
authors.initials: A
authors.lastname: Sasaki
authors.affiliation: b Food Processing Research Station , Akita Research Institute of Food and Brewing , Akita , Japan.
authors.forename: Kazuyuki
authors.initials: K
authors.lastname: Hiwatashi
authors.affiliation: b Food Processing Research Station , Akita Research Institute of Food and Brewing , Akita , Japan.
authors.forename: Masanori
authors.initials: M
authors.lastname: Kumagai
authors.affiliation: b Food Processing Research Station , Akita Research Institute of Food and Brewing , Akita , Japan.
authors.forename: Keishi
authors.initials: K
authors.lastname: Hata
authors.affiliation: a Graduate School of Bioresource Sciences , Akita Prefectural University , Akita , Japan.
authors.forename: Masayuki
authors.initials: M
authors.lastname: Kobayashi
mesh_terms: D002454:Cell Differentiation; D005786:Gene Expression Regulation; D056945:Hep G2 Cells; D051538:Hepatocyte Nuclear Factor 1-alpha / Q000235:genetics*; D051542:Hepatocyte Nuclear Factor 3-beta / Q000235:genetics; D051557:Hepatocyte Nuclear Factor 4 / Q000235:genetics; D022781:Hepatocytes / Q000166:cytology / Q000378:metabolism; D006801:Humans; D008074:Lipoproteins / Q000096:biosynthesis*; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism*
chemical_list: C496502:FOXA2 protein, human; C496592:HNF4A protein, human; D051538:Hepatocyte Nuclear Factor 1-alpha; D051557:Hepatocyte Nuclear Factor 4; D008074:Lipoproteins; D051542:Hepatocyte Nuclear Factor 3-beta
keywords: ApoB100; HNF1α; HNF4α; MTP; lipoprotein production