title: In vitro evaluation of gelatin and chitosan electrospun fibres as an artificial guide in peripheral nerve repair: a comparative study.
abstract: Random and aligned gelatin (GL) and chitosan (CS) nano-fibres have been prepared by electrospinning tuning the collector rotation speed. The effect of fibre alignment on cell adhesion and proliferation was assessed in vitro by using different Schwann cell (SC) and neuronal models. Moreover, actin cytoskeleton organization, lamellipodia and filipodia formation, and axon outgrowth were evaluated. GL and CS fibres induced similar adhesion and proliferation rates. GL and CS random fibres promoted higher adhesion and proliferation rates induction in comparison to the aligned ones, although GL and CS fibres alignment resulted in SC and axon-oriented growth. Filipodia formation was higher on aligned fibres, suggesting that these substrates can promote higher cell migration in comparison to random ones. 50B11 (neuronal cell line) differentiation was higher on GL fibres, whereas no differences were observed in dorsal root ganglia explants model. These data suggest that both GL and CS fibres can be promising substrates to be used in peripheral nerve reconstruction. Copyright © 2016 John Wiley & Sons, Ltd.
authors.affiliation: Department of Clinical and Biological Sciences, University of Torino, Orbassano, 10043, Italy.|Neuroscience Institute of the Cavalieri-Ottolenghi Foundation, University of Torino, Orbassano, 10043, Italy.
authors.forename: S
authors.initials: S
authors.lastname: Gnavi
authors.affiliation: Department of Clinical and Biological Sciences, University of Torino, Orbassano, 10043, Italy.|Neuroscience Institute of the Cavalieri-Ottolenghi Foundation, University of Torino, Orbassano, 10043, Italy.
authors.forename: B E
authors.initials: BE
authors.lastname: Fornasari
authors.affiliation: Politecnico di Torino, Department of Mechanical and Aerospace Engineering, Politecnico of Torino, Torino, 10100, Italy.
authors.forename: C
authors.initials: C
authors.lastname: Tonda-Turo
authors.affiliation: Politecnico di Torino, Department of Mechanical and Aerospace Engineering, Politecnico of Torino, Torino, 10100, Italy.
authors.forename: R
authors.initials: R
authors.lastname: Laurano
authors.affiliation: Nanostructured Interfaces and Surfaces, Department of Chemistry, University of Torino, Torino, 10100, Italy.
authors.forename: M
authors.initials: M
authors.lastname: Zanetti
authors.affiliation: Politecnico di Torino, Department of Mechanical and Aerospace Engineering, Politecnico of Torino, Torino, 10100, Italy.|CNR-IPCF UOS, Pisa, 56124, Italy.
authors.forename: G
authors.initials: G
authors.lastname: Ciardelli
authors.affiliation: Department of Clinical and Biological Sciences, University of Torino, Orbassano, 10043, Italy.|Neuroscience Institute of the Cavalieri-Ottolenghi Foundation, University of Torino, Orbassano, 10043, Italy.
authors.forename: S
authors.initials: S
authors.lastname: Geuna
mesh_terms: D008841:Actin Cytoskeleton / Q000187:drug effects / Q000378:metabolism; D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D048271:Chitosan / Q000494:pharmacology*; D005260:Female; D022001:Focal Adhesions / Q000187:drug effects / Q000378:metabolism; D005780:Gelatin / Q000494:pharmacology*; D057139:Nanofibers / Q000737:chemistry / Q000648:ultrastructure; D009416:Nerve Regeneration / Q000187:drug effects*; D016501:Neurites / Q000187:drug effects / Q000378:metabolism; D011554:Pseudopodia / Q000187:drug effects / Q000378:metabolism; D017208:Rats, Wistar; D012583:Schwann Cells / Q000166:cytology / Q000187:drug effects; D017550:Spectroscopy, Fourier Transform Infrared; D034421:Sus scrofa; D023822:Tissue Engineering / Q000379:methods*
chemical_list: D005780:Gelatin; D048271:Chitosan
keywords: aligned fibres; biomimetic materials; chitosan; electrospun fibres; gelatin; peripheral nerve repair; random fibres