title: Selection of DNA aptamers against penicillin G using Capture-SELEX for the development of an impedimetric sensor.
abstract: This paper describes for the first time the selection of aptamers selective to penicillin. Aptamers were selected using a specific process called Capture-SELEX (Systematic Evolution of Ligands by Exponential Enrichment). This technique is based on the selection of DNA aptamers using penicillin G in solution while the ssDNA library is fixed on a support. One aptamer showing a good affinity to penicillin was finally selected and tested in electrochemical sensor configuration, using electrochemical impedance spectroscopy as detection technique. The developed aptasensor allowed the detection of penicillin in a wide concentration range, comprised between 0.4 and 1000µgL-1Such performance was compatible with milk analysis, as the maximum residue limit tolerated in this matrix is 4µgL-1. The selectivity of the developed sensor was also studied, showing that the sensor was also able to bind other beta-lactam antibiotics, although with a weaker affinity. Finally the sensor was used for detection of penicillin G in milk. It was shown that a simple sample treatment with isopropanol followed by filtration was sufficient to eliminate matrix effects, allowing the determination of penicillin in milk at concentrations compatible with legislation requirements.
authors.affiliation: Laboratoire BAE, Université de Perpignan Via Domitia, 52 Avenue Paul Alduy, 66860 Perpignan Cedex 9, France.
authors.forename: Nathalie
authors.initials: N
authors.lastname: Paniel
authors.affiliation: Laboratoire BAE, Université de Perpignan Via Domitia, 52 Avenue Paul Alduy, 66860 Perpignan Cedex 9, France.
authors.forename: Georges
authors.initials: G
authors.lastname: Istamboulié
authors.affiliation: Laboratoire BAE, Université de Perpignan Via Domitia, 52 Avenue Paul Alduy, 66860 Perpignan Cedex 9, France.
authors.forename: Athar
authors.initials: A
authors.lastname: Triki
authors.affiliation: Laboratoire BAE, Université de Perpignan Via Domitia, 52 Avenue Paul Alduy, 66860 Perpignan Cedex 9, France.
authors.forename: Clément
authors.initials: C
authors.lastname: Lozano
authors.affiliation: Laboratoire BAE, Université de Perpignan Via Domitia, 52 Avenue Paul Alduy, 66860 Perpignan Cedex 9, France.
authors.forename: Lise
authors.initials: L
authors.lastname: Barthelmebs
authors.affiliation: Laboratoire BAE, Université de Perpignan Via Domitia, 52 Avenue Paul Alduy, 66860 Perpignan Cedex 9, France. Electronic address: noguer@univ-perp.fr.
authors.forename: Thierry
authors.initials: T
authors.lastname: Noguer
mesh_terms: D000818:Animals; D052157:Aptamers, Nucleotide / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D001483:Base Sequence; D001667:Binding, Competitive; D015374:Biosensing Techniques / Q000379:methods*; D004277:DNA, Single-Stranded / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D058266:Dielectric Spectroscopy / Q000379:methods*; D008892:Milk / Q000737:chemistry; D010400:Penicillin G / Q000032:analysis / Q000737:chemistry* / Q000378:metabolism; D015203:Reproducibility of Results; D052156:SELEX Aptamer Technique / Q000379:methods*; D047090:beta-Lactams / Q000032:analysis / Q000737:chemistry / Q000378:metabolism
chemical_list: D052157:Aptamers, Nucleotide; D004277:DNA, Single-Stranded; D047090:beta-Lactams; D010400:Penicillin G
keywords: Aptamers; Capture-SELEX; Impedimetric sensor; Milk analysis; Penicillin