title: Beneficial effect of coenzyme Q10injection on nitric oxide -related dilation of the rat aorta.
abstract: This study examined whether coenzyme Q10can improve nitric oxide (NO)-dependent vasodilatation in the rat aorta after pre-incubation or intravenous administration. In initial experiments, intact isolated aortic rings were incubated with coenzyme Q10or L-arginine. In further experiments, coenzyme Q10was administered intravenously in anesthetized rats, then in 2h aorta was isolated. In both cases, after preliminary preparation the isolated aortic rings were tested for acetylcholine-induced NO-dependent relaxation. Acetylcholine elicited concentration-dependent relaxation of phenylephine precontracted aortic rings. Relaxant responses to acetylcholine were markedly potentiated after pre-incubation with coenzyme Q10or L-arginine. The maximum relaxant responses (%) were significantly increased from 64.1±5.3 (control) to 89.8±3.0 and 83.6±3.0 (coenzyme Q10and L-arginine, respectively). pD2(-lgEC50) value in control study was 5.81±0.28, after pretreatment with coenzyme Q10or L-arginine were 7.59±0.16 and 7.26±0.32, respectively. There was no difference between coenzyme Q10and L-arginine groups. After intravenous administration, the relaxant responses to acetylcholine were significantly increased in coenzyme Q10-treated group (94.2±2.0) compared with controls (68.1±4.4). pD2values were also different between control and treatment groups (5.79±0.29 vs. 8.14±0.65, respectively). Thus, coenzyme Q10improved NO-mediated vasodilation in rat aorta in magnitude close to the effects of L-arginine - substrate for eNOS. Our data first show that exogenous coenzyme Q10through intravenous administration is able to improve rapidly NO-dependent vasodilation in rat aorta, likely due to accumulation of coenzyme Q10in the vessel wall. Improvement of endothelial function can contribute, at least in part, to beneficial effects of coenzyme Q10in cardiovascular diseases associated with endothelial dysfunction.
authors.affiliation: Department of Pharmacology, Moscow State University, Lomonosovsky prosp. 31/5, 119192 Moscow, Russia. Electronic address: lali_k@mail.ru.
authors.forename: Larisa P
authors.initials: LP
authors.lastname: Kozaeva
authors.affiliation: Department of Pharmacology, Moscow State University, Lomonosovsky prosp. 31/5, 119192 Moscow, Russia.
authors.forename: Evgeniya A
authors.initials: EA
authors.lastname: Gorodetskaya
authors.affiliation: Department of Institute of Experimental Cardiology, Russian Cardiology Research and Production Complex, 3rd Cherepkovskaya str.15a, 121552 Moscow, Russia.
authors.forename: Enno K
authors.initials: EK
authors.lastname: Ruuge
authors.affiliation: Department of Pharmacology, Moscow State University, Lomonosovsky prosp. 31/5, 119192 Moscow, Russia.
authors.forename: Elena I
authors.initials: EI
authors.lastname: Kalenikova
authors.affiliation: Department of Pharmacology, Moscow State University, Lomonosovsky prosp. 31/5, 119192 Moscow, Russia.
authors.forename: Oleg S
authors.initials: OS
authors.lastname: Medvedev
mesh_terms: D000818:Animals; D001013:Aorta, Thoracic / Q000187:drug effects / Q000502:physiology; D007267:Injections; D008297:Male; D009569:Nitric Oxide / Q000494:pharmacology*; D051381:Rats; D014451:Ubiquinone / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000494:pharmacology; D014664:Vasodilation / Q000187:drug effects*
chemical_list: D014451:Ubiquinone; D009569:Nitric Oxide; C024989:coenzyme Q10
keywords: Coenzyme Q(10); Endothelial dysfunction; Intravenous administration; Isolated rat aorta; NO-dependent relaxation