title: Modulation of Cell Attachment, Proliferation, and Angiogenesis by Decellularized, Dehydrated Human Amniotic Membrane in In Vitro Models.
abstract: BACKGROUND
  Decellularized, dehydrated human amniotic membrane (DDHAM) is an extracellular matrix devoid of cells, cell debris, and growth factors. This study examines the effect of cell attachment to the DDHAM and the induced cellular responses.
  
  
  METHODS
  The cell types employed in this study were human dermal fibroblasts (HDF), human epithelial keratinocytes (HEK), and human dermal microvascular endothelial cells (HDMEC), all of which play critical roles in the wound healing process. Further, the DDHAM was compared to a dehydrated human amnion/chorion membrane (dHACM), which contains and releases biological entities including growth factors and cytokines. The HDF and HEK were cultured on the DDHAM and the dHACM, and cell imaging and proliferation assays were performed to evaluate cell attachment to and the ability to proliferate on the DDHAM relative to the dHACM. In addition, the effect of soluble factors released by the DDHAM and the dHACM on cell survival, attachment, and proliferation were examined. The authors also evaluated the effect of soluble factors produced by culturing cells on the DDHAM in in vitro functional assays, including cell survival and endothelial cell migration in a wound closure angiogenesis assay.
  
  
  RESULTS
  The HDF and HEK cells readily attached to and proliferated on the DDHAM, while the dHACM did not support cell attachment and proliferation when cultured under the same conditions. Soluble factors secreted when HDF were cultured on the DDHAM enhanced both endothelial cell and keratinocyte survival and endothelial cell migration in a wound closure assay.
  
  
  CONCLUSIONS
  Although DDHAM is only an extracellular matrix and serves primarily as a scaffold, it has sufficient cues to allow for cell attachment and proliferation. Further, the biological entities released as a consequence of cell attachment promote cell survival and migration.
authors.affiliation: Celgene Cellular Therapeutics, Warren, NJ.
authors.forename: Xuan
authors.initials: X
authors.lastname: Guo
authors.affiliation: Celgene Cellular Therapeutics, Warren, NJ.
authors.forename: Aleksandr
authors.initials: A
authors.lastname: Kaplunovsky
authors.affiliation: Celgene Cellular Therapeutics, Warren, NJ.
authors.forename: Raihana
authors.initials: R
authors.lastname: Zaka
authors.affiliation: Celgene Cellular Therapeutics, Warren, NJ.
authors.forename: Chuan
authors.initials: C
authors.lastname: Wang
authors.affiliation: Celgene Cellular Therapeutics, Warren, NJ.
authors.forename: Hemlata
authors.initials: H
authors.lastname: Rana
authors.affiliation: Celgene Cellular Therapeutics, Warren, NJ.
authors.forename: Jeffrey
authors.initials: J
authors.lastname: Turner
authors.affiliation: Celgene Cellular Therapeutics, Warren, NJ.
authors.forename: Qian
authors.initials: Q
authors.lastname: Ye
authors.affiliation: Celgene Cellular Therapeutics, Warren, NJ.
authors.forename: Ivana
authors.initials: I
authors.lastname: Djuretic
authors.affiliation: Celgene Cellular Therapeutics, Warren, NJ.
authors.forename: Joseph
authors.initials: J
authors.lastname: Gleason
authors.affiliation: Celgene Cellular Therapeutics, Warren, NJ.
authors.forename: Vladimir
authors.initials: V
authors.lastname: Jankovic
authors.affiliation: Alliqua BioMedical, Yardley, PA.
authors.forename: Janice M
authors.initials: JM
authors.lastname: Smiell
authors.affiliation: Celgene Cellular Therapeutics, Warren, NJ.
authors.forename: Mohit
authors.initials: M
authors.lastname: Bhatia
authors.affiliation: Celgene Cellular Therapeutics, Warren, NJ.
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Hofgartner
authors.affiliation: Celgene Cellular Therapeutics, Warren, NJ.
authors.forename: Robert
authors.initials: R
authors.lastname: Hariri
mesh_terms: D064591:Allografts; D000650:Amnion / Q000737:chemistry*; D001684:Biological Dressings; D002448:Cell Adhesion; D049109:Cell Proliferation; D021982:Cell-Matrix Junctions; D005109:Extracellular Matrix / Q000378:metabolism / Q000648:ultrastructure; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism; D006801:Humans; D066298:In Vitro Techniques; D018919:Neovascularization, Physiologic; D016896:Treatment Outcome; D014945:Wound Healing; D014947:Wounds and Injuries / Q000473:pathology*