title: Advanced oxidation protein products induce chondrocyte death through a redox-dependent, poly (ADP-ribose) polymerase-1-mediated pathway.
abstract: This study aimed to investigate the effect of AOPPs on apoptosis in human chondrocytes. Chondrocytes were treated with AOPPs. Cell death, nicotinamide adenine dinucleotide phosphate (NADPH) oxidase activity, reactive oxygen species (ROS) generation, and the expression of apoptotic proteins were detected in vitro. AOPPs levels were detected by colorimetric method. The results in vitro demonstrated that AOPPs induced cell death in human chondrocyte through a redox-dependent pathway, including RAGE-mediated, NADPH oxidase-dependent ROS generation, and poly (ADP-ribose) polymerase-1 (PARP-1) activation. Targeting AOPPs-induced cellular mechanisms might emerge as a promising therapeutic option for patients with RA.
authors.affiliation: Department of Orthopaedics, The 175th Hospital of PLA, Affiliated Dongnan Hospital of Xiamen University, Zhangzhou, Fujian, China.
authors.forename: Wenbin
authors.initials: W
authors.lastname: Ye
authors.affiliation: Department of Orthopaedic Spinal Surgery, Nanfang Hospital, Southern Medical University, 1838 North Guangzhou Avenue, Guangzhou, 510515, China.
authors.forename: Zhaoming
authors.initials: Z
authors.lastname: Zhong
authors.affiliation: Department of Orthopaedic Spinal Surgery, Nanfang Hospital, Southern Medical University, 1838 North Guangzhou Avenue, Guangzhou, 510515, China.
authors.forename: Siyuan
authors.initials: S
authors.lastname: Zhu
authors.affiliation: Department of Orthopaedic Spinal Surgery, Nanfang Hospital, Southern Medical University, 1838 North Guangzhou Avenue, Guangzhou, 510515, China.
authors.forename: Shuai
authors.initials: S
authors.lastname: Zheng
authors.affiliation: Department of Orthopedic Joint Surgery, Nanfang Hospital, Southern Medical University, Guangzhou, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Xiao
authors.affiliation: Department of Pharmacy, Nanfang Hospital, Southern Medical University, Guangzhou, China.
authors.forename: Shaolian
authors.initials: S
authors.lastname: Song
authors.affiliation: Department of Orthopaedic Spinal Surgery, Nanfang Hospital, Southern Medical University, 1838 North Guangzhou Avenue, Guangzhou, 510515, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Yu
authors.affiliation: Department of Orthopaedic Spinal Surgery, Nanfang Hospital, Southern Medical University, 1838 North Guangzhou Avenue, Guangzhou, 510515, China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Wu
authors.affiliation: Department of Orthopaedic Spinal Surgery, Nanfang Hospital, Southern Medical University, 1838 North Guangzhou Avenue, Guangzhou, 510515, China.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Lin
authors.affiliation: Department of Orthopaedic Spinal Surgery, Nanfang Hospital, Southern Medical University, 1838 North Guangzhou Avenue, Guangzhou, 510515, China. jianting_chen@sina.com.
authors.forename: Jianting
authors.initials: J
authors.lastname: Chen
mesh_terms: D062107:Advanced Oxidation Protein Products / Q000235:genetics* / Q000378:metabolism; D017209:Apoptosis / Q000235:genetics*; D019902:Chondrocytes / Q000378:metabolism*; D006801:Humans; D010084:Oxidation-Reduction; D000071137:Poly (ADP-Ribose) Polymerase-1 / Q000235:genetics* / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism; D015398:Signal Transduction
chemical_list: D062107:Advanced Oxidation Protein Products; D017382:Reactive Oxygen Species; D000071137:Poly (ADP-Ribose) Polymerase-1
keywords: Advanced oxidation protein production (AOPPs); Apoptosis; Chondrocytes; Oxidative stress; PARP-1; Rheumatoid arthritis