title: Effect of Follicle Stimulating Hormone Receptor Gene Polymorphisms in Cervical Cancer Risk.
abstract: For the first time in the word, we investigated the association between five FSHR polymorphisms with the risk of cervical cancer among Tunisians. Study subjects comprised 112 Cervical Cancer (CC) patients and 164 control women. Genotyping of FSHR rs6166, rs1007541, rs11692782, rs2055571 and rs1394205 variants was done by realtime PCR, with defined clusters. The allelic distributions of the tested FSHR SNPs were comparable between CC patients and control women. In contrast, the heterozygous genotype of rs1007541 was associated with 1.8-fold increased risk of CC. Stratification according to FIGO staging revealed that the minor allele of rs1007541 was more frequent among advanced tumor stage patients, with 11-fold increased risk of CC [P < 0.0001; OR (95 % CI) = 11.32 (7.46-17.18)]. However, no significant allelic association was revealed in the rest of analyzed FSHR SNPs. Haploview analysis showed high Linkage disequilibrium (LD) between rs2055571 and rs1394205. Haplotype analysis revealed a lack of association between cases and controls. However, analysis of CC patient subgroups demonstrated enrichment of GGTAG haplotype in early tumor stage [P = 0.025; OR (95 % CI) = 0.07 (0.01-0.70)]. The FSHR variants and haplotypes may be a genetic markers for CC susceptibility and evolution among Tunisian women.
authors.affiliation: Department of Biology, Faculty of Sciences of Tunis, Laboratory of Mycology, Pathologies and Biomarkers: LR16ES05, El Manar University, 1092, Tunis, Tunisia. ZIDISABRINA86@gmail.com.
authors.forename: Sabrina
authors.initials: S
authors.lastname: Zidi
authors.affiliation: Department of Biology, Faculty of Sciences of Tunis, Laboratory of Mycology, Pathologies and Biomarkers: LR16ES05, El Manar University, 1092, Tunis, Tunisia.
authors.forename: Mouna
authors.initials: M
authors.lastname: Stayoussef
authors.affiliation: Department of Medical Biochemistry, Arabian Gulf University, Manama, Bahrain.
authors.forename: Bano L
authors.initials: BL
authors.lastname: Alsaleh
authors.affiliation: Military Hospital of Tunis, Laboratory of Immunology, Tunis, Tunisia.
authors.forename: Ezzedine
authors.initials: E
authors.lastname: Gazouani
authors.affiliation: Salah Azeiz Oncology Institute, Tunis, Tunisia.
authors.forename: Amel
authors.initials: A
authors.lastname: Mezlini
authors.affiliation: Department of Medical Biochemistry, Arabian Gulf University, Manama, Bahrain.
authors.forename: Bashayer H
authors.initials: BH
authors.lastname: Ebrahim
authors.affiliation: Department of Biology, Faculty of Sciences of Tunis, Laboratory of Mycology, Pathologies and Biomarkers: LR16ES05, El Manar University, 1092, Tunis, Tunisia.
authors.forename: Besma
authors.initials: B
authors.lastname: Yacoubi-Loueslati
authors.affiliation: Department of Medical Biochemistry, Arabian Gulf University, Manama, Bahrain.
authors.forename: Wassim Y
authors.initials: WY
authors.lastname: Almawi
mesh_terms: D000483:Alleles; D016022:Case-Control Studies; D005260:Female; D005787:Gene Frequency / Q000235:genetics; D020022:Genetic Predisposition to Disease / Q000235:genetics*; D005838:Genotype; D006579:Heterozygote; D006801:Humans; D015810:Linkage Disequilibrium / Q000235:genetics; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D011962:Receptors, FSH / Q000235:genetics*; D012189:Retrospective Studies; D012306:Risk; D002583:Uterine Cervical Neoplasms / Q000235:genetics*
chemical_list: D011962:Receptors, FSH
keywords: Cervical cancer; FIGO stages; FSHR; Haplotypes; Polymorphisms; Tunisians