title: An ultra-sensitive and colorimetric sensor for copper and iron based on glutathione-functionalized gold nanoclusters.
abstract: Here, we report an ultra-sensitive and colorimetric sensor for the detection of Fe3+or Cu2+successively using glutathione-functionalized Au nanoclusters (GSH-AuNCs). For GSH-AuNCs can catalytically oxidize peroxidase substrates, such as 3, 3', 5, 5'-tetramethylbenzidine (TMB), colored products are formed in the presence of H2O2. While upon the addition of Fe3+or Cu2+into the GSH-AuNCs-TMB-H2O2system, diverse color and absorbance of the system was obtained due to the self oxidation of Fe3+and the inhibition of peroxidase-like activity of GSH-AuNCs. With the introduction of ethylene diamine tetraacetic acid (EDTA) or ammonium fluoride (NH4F) to GSH-AuNCs-TMB-H2O2+Cu2+system or GSH-AuNCs-TMB-H2O2+Fe3+system respectively, a restoration of color and absorbance of system was realized. On the basis of above phenomenon, a colorimetric and quantitative approach for detecting Fe3+and Cu2+was developed with detection limit of 1.25 × 10-9M and 1.25 × 10-10M respectively. Moreover, the concentration of Fe3+and Cu2+in human serums was also accurate quantified by this method. So this design strategy realized the simple and simultaneous detection of Fe3+and Cu2+, suggesting significant potential in clinical diagnosis.
authors.affiliation: School of Life Sciences, Tianjin University, Tianjin Engineering Center of Micro-Nano Biomaterials and Detection-Treatment Technology (Tianjin), 92 Weijin Road, Nankai District, Tianjin 300072, China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Zhao
authors.affiliation: Medical Laboratory, Tianjin Medical University General Hospital, Tianjin, 300052, China.
authors.forename: Huan
authors.initials: H
authors.lastname: Yan
authors.affiliation: Bioscience(Tianjin) Diagnostic Technology CO., LTD, Tianjin, 300300, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Liu
authors.affiliation: School of Life Sciences, Tianjin University, Tianjin Engineering Center of Micro-Nano Biomaterials and Detection-Treatment Technology (Tianjin), 92 Weijin Road, Nankai District, Tianjin 300072, China.
authors.forename: Yingyi
authors.initials: Y
authors.lastname: Yao
authors.affiliation: School of Life Sciences, Tianjin University, Tianjin Engineering Center of Micro-Nano Biomaterials and Detection-Treatment Technology (Tianjin), 92 Weijin Road, Nankai District, Tianjin 300072, China.
authors.forename: Yudong
authors.initials: Y
authors.lastname: Wu
authors.affiliation: School of Life Sciences, Tianjin University, Tianjin Engineering Center of Micro-Nano Biomaterials and Detection-Treatment Technology (Tianjin), 92 Weijin Road, Nankai District, Tianjin 300072, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Zhang
authors.affiliation: School of Life Sciences, Tianjin University, Tianjin Engineering Center of Micro-Nano Biomaterials and Detection-Treatment Technology (Tianjin), 92 Weijin Road, Nankai District, Tianjin 300072, China.
authors.forename: Hengxuan
authors.initials: H
authors.lastname: Li
authors.affiliation: School of Life Sciences, Tianjin University, Tianjin Engineering Center of Micro-Nano Biomaterials and Detection-Treatment Technology (Tianjin), 92 Weijin Road, Nankai District, Tianjin 300072, China. Electronic address: gongxiaoqun@tju.edu.cn.
authors.forename: Xiaoqun
authors.initials: X
authors.lastname: Gong
authors.affiliation: School of Life Sciences, Tianjin University, Tianjin Engineering Center of Micro-Nano Biomaterials and Detection-Treatment Technology (Tianjin), 92 Weijin Road, Nankai District, Tianjin 300072, China. Electronic address: jinchang@tju.edu.cn.
authors.forename: Jin
authors.initials: J
authors.lastname: Chang
mesh_terms: D003124:Colorimetry / Q000379:methods*; D003300:Copper / Q000032:analysis*; D005978:Glutathione / Q000737:chemistry*; D006046:Gold / Q000737:chemistry*; D007501:Iron / Q000032:analysis*; D057230:Limit of Detection; D053768:Metal Nanoparticles / Q000737:chemistry*
chemical_list: D006046:Gold; D003300:Copper; D007501:Iron; D005978:Glutathione
keywords: Au nanoclusters; Cu(2+); Fe(3+); Peroxidase-like activity; TMB