title: Multifunctional Thioredoxin-Like Protein from the Gastrointestinal Parasitic NematodesStrongyloides rattiandTrichuris suisAffects Mucosal Homeostasis.
abstract: The cellular redox state is important for the regulation of multiple functions and is essential for the maintenance of cellular homeostasis and antioxidant defense. In the excretory/secretory (E/S) products ofStrongyloides rattiandTrichuris suissequences for thioredoxin (Trx) and Trx-like protein (Trx-lp) were identified. To characterize the antioxidant Trx-lp and its interaction with the parasite's mucosal habitat,S. rattiandT. suisTrx-lps were cloned and recombinantly expressed. The primary antioxidative activity was assured by reduction of insulin and IgM. Further analysis applying anin vitromucosal 3D-cell culture model revealed that the secreted Trx-lps were able to bind to monocytic and intestinal epithelial cells and induce the time-dependent release of cytokines such as TNF-α, IL-22, and TSLP. In addition, the redox proteins also possessed chemotactic activity for monocytic THP-1 cells and fostered epithelial wound healing activity. These results confirm that the parasite-secreted Trx-lps are multifunctional proteins that can affect the host intestinal mucosa.
authors.affiliation: Department of Molecular Physiology, Westfälische Wilhelms-University, Münster, Germany; Department of Molecular Medicine, Bernhard Nocht Institute for Tropical Medicine, Hamburg, Germany.
authors.forename: Dana
authors.identifier: 0000-0002-9989-7764
authors.initials: D
authors.lastname: Ditgen
authors.affiliation: Department of Molecular Physiology, Westfälische Wilhelms-University, Münster, Germany; Department of Molecular Medicine, Bernhard Nocht Institute for Tropical Medicine, Hamburg, Germany.
authors.forename: Emmanuela M
authors.identifier: 0000-0002-5616-5969
authors.initials: EM
authors.lastname: Anandarajah
authors.affiliation: Department of Tissue Engineering and Regenerative Medicine (TERM), University of Würzburg, Germany.
authors.forename: Jan
authors.initials: J
authors.lastname: Hansmann
authors.affiliation: Institute for Biochemistry and Molecular Biology, University of Bonn, Bonn, Germany.
authors.forename: Dominic
authors.initials: D
authors.lastname: Winter
authors.affiliation: Ovamed GmbH, Hamburg, Germany.
authors.forename: Guido
authors.initials: G
authors.lastname: Schramm
authors.affiliation: Department of Molecular Medicine, Bernhard Nocht Institute for Tropical Medicine, Hamburg, Germany.
authors.forename: Klaus D
authors.initials: KD
authors.lastname: Erttmann
authors.affiliation: Department of Molecular Physiology, Westfälische Wilhelms-University, Münster, Germany.
authors.forename: Eva
authors.initials: E
authors.lastname: Liebau
authors.affiliation: Department of Molecular Medicine, Bernhard Nocht Institute for Tropical Medicine, Hamburg, Germany.
authors.forename: Norbert W
authors.identifier: 0000-0003-4291-5009
authors.initials: NW
authors.lastname: Brattig