title: Proteomic and physiological approach reveals drought-induced changes in rapeseeds: Water-saver and water-spender strategy.
abstract: The cultivar-dependent differences in Brassica napus L. seed yield are significantly affected by drought stress. Here, the response of leaf proteome to long-term drought (28days) was studied in cultivars (cvs): Californium (C), Cadeli (D), Navajo (N), and Viking (V). Analysis of twenty-four 2-D DIGE gels revealed 134 spots quantitatively changed at least 2-fold; from these, 79 proteins were significantly identified by MALDI-TOF/TOF. According to the differences in water use, the cultivars may be assigned to two categories: water-savers or water-spenders. In the water-savers group (cvs C+D), proteins related to nitrogen assimilation, ATP and redox homeostasis were increased under stress, while in the water-spenders category (cvs N+V), carbohydrate/energy, photosynthesis, stress related and rRNA processing proteins were increased upon stress. Taking all data together, we indicated cv C as a drought-adaptable water-saver, cv D as a medium-adaptable water-saver, cv N as a drought-adaptable water-spender, and cv V as a low-adaptable drought sensitive water-spender rapeseed. Proteomic data help to evaluate the impact of drought and the extent of genotype-based adaptability and contribute to the understanding of their plasticity. These results provide new insights into the provenience-based drought acclimation/adaptation strategy of contrasting winter rapeseeds and link data at gasometric, biochemical, and proteome level.
  
  
  
  Soil moisture deficit is a real problem for every crop. The data in this study demonstrates for the first time that in stem-prolongation phase cultivars respond to progressive drought in different ways and at different levels. Analysis of physiological and proteomic data showed two different water regime-related strategies: water-savers and spenders. However, not only water uptake rate itself, but also individual protein abundances, gasometric and biochemical parameters together with final biomass accumulation after stress explained genotype-based responses. Interestingly, under a mixed climate profile, both water-use patterns (savers or spenders) can be appropriate for drought adaptation. These data suggest, than complete "acclimation image" of rapeseeds in stem-prolongation phase under drought could be reached only if these characteristics are taken, explained and understood together.
authors.affiliation: Crop Research Institute, Department of Genetics and Plant Breeding, Drnovská 507/73, Prague, Czech Republic; Charles University, Department of Experimental Plant Biology, Viničná 5, Prague, Czech Republic. Electronic address: dehydrins@gmail.com.
authors.forename: Milan Oldřich
authors.initials: MO
authors.lastname: Urban
authors.affiliation: Czech University of Life Sciences Prague, Department of Genetics and Breeding, Kamýcká 129, Prague, Czech Republic.
authors.forename: Jakub
authors.initials: J
authors.lastname: Vašek
authors.affiliation: Crop Research Institute, Department of Genetics and Plant Breeding, Drnovská 507/73, Prague, Czech Republic.
authors.forename: Miroslav
authors.initials: M
authors.lastname: Klíma
authors.affiliation: Charles University, Department of Experimental Plant Biology, Viničná 5, Prague, Czech Republic.
authors.forename: Jana
authors.initials: J
authors.lastname: Krtková
authors.affiliation: Crop Research Institute, Department of Genetics and Plant Breeding, Drnovská 507/73, Prague, Czech Republic.
authors.forename: Klára
authors.initials: K
authors.lastname: Kosová
authors.affiliation: Crop Research Institute, Department of Genetics and Plant Breeding, Drnovská 507/73, Prague, Czech Republic.
authors.forename: Ilja Tom
authors.initials: IT
authors.lastname: Prášil
authors.affiliation: Crop Research Institute, Department of Genetics and Plant Breeding, Drnovská 507/73, Prague, Czech Republic.
authors.forename: Pavel
authors.initials: P
authors.lastname: Vítámvás
mesh_terms: D000222:Adaptation, Physiological; D029741:Brassica rapa / Q000737:chemistry* / Q000502:physiology; D055864:Droughts; D015180:Electrophoresis, Gel, Two-Dimensional; D010940:Plant Proteins / Q000032:analysis; D040901:Proteomics / Q000379:methods*; D013312:Stress, Physiological; D053719:Tandem Mass Spectrometry; D014867:Water / Q000378:metabolism*
chemical_list: D010940:Plant Proteins; D014867:Water
keywords: 2D-DIGE; Brassica napus; Proteomics; Soil moisture deficit; Water-saver; Water-spender