title: Effect of circulating tissue factor on hypercoagulability in type 2 diabetes mellitus studied by rheometry and dielectric blood coagulometry.
abstract: Hypercoagulability in type 2 diabetes mellitus (T2DM) patients increases their risk of cardiovascular diseases.
  
  
  
  The aim of this work was to investigate the hypercoagulation mechanism in T2DM patients in terms of circulating tissue factor (TF).
  
  
  
  Whole blood coagulation tests by damped oscillation rheometry and dielectric blood coagulometry (DBCM) were performed.
  
  
  
  The average coagulation time was significantly shorter for T2DM patients than for healthy controls. In vitro addition of either anti-TF or anti-activated factor VII (FVIIa) antibody to hypercoagulable blood samples prolonged coagulation times for one group of patients, while coagulation times remained short for another group. The levels of circulating TF were estimated in the former group by measuring the coagulation times for blood samples from healthy subjects with addition of various concentrations of TF and comparing them with the coagulation times for the group. The results indicated that the levels of circulating TF were on the order of subpicomolar at most.
  
  
  
  Circulating TF is at least partially responsible for a hypercoagulable group of T2DM patients, while an abnormality in the intrinsic coagulation pathway probably occurs in the other group.
authors.affiliation: Department of Endocrinology and Metabolism, Tokyo Medical and Dental University, Tokyo, Japan.
authors.forename: Isao
authors.initials: I
authors.lastname: Uchimura
authors.affiliation: RIKEN (The Institute of Physical and Chemical Research), Wako, Saitama, Japan.
authors.forename: Makoto
authors.initials: M
authors.lastname: Kaibara
authors.affiliation: Department of Pediatrics, Tokyo Medical and Dental University, Tokyo, Japan.|Department of Pediatrics, Musashino Red Cross Hospital, Musashino-city, Tokyo, Japan.
authors.forename: Masayuki
authors.initials: M
authors.lastname: Nagasawa
authors.affiliation: LE Development Department, R&D Division, Medical Business Unit, Imaging Products & Solutions Sector, Sony Corporation, Tokyo, Japan.
authors.forename: Yoshihito
authors.initials: Y
authors.lastname: Hayashi
mesh_terms: D001777:Blood Coagulation; D001780:Blood Coagulation Tests / Q000379:methods*; D048909:Diabetes Complications / Q000175:diagnosis* / Q000473:pathology; D003924:Diabetes Mellitus, Type 2 / Q000175:diagnosis* / Q000473:pathology; D058266:Dielectric Spectroscopy / Q000379:methods*; D006801:Humans; D019851:Thrombophilia / Q000150:complications*; D013925:Thromboplastin / Q000378:metabolism*
chemical_list: D013925:Thromboplastin
keywords: Dielectric spectroscopy; coagulation time; extrinsic/intrinsic coagulation pathway; hypercoagulable state