title: Screening and Identification of Novel Ochratoxin A-Producing Fungi from Grapes.
abstract: Ochratoxin A (OTA) contamination has been established as a world-wide problem. In this study, the strains with the ability of OTA production were screened by analyzing the green fluorescence of the isolates colonies from the grapes in Zhenjiang with 365 nm UV light and confirmed by HPLC with fluorescent detection (HPLC-FLD). The results showed that seven isolates acquired the characteristic of the fluorescence, of which only five showed the ability of OTA production as confirmed by HPLC-FLD analysis. The five OTA-producing strains were identified based on comparative sequence analysis of three conserved genes (ITS,BenAandRPB2) of the strains, and they areTalaromyces rugulosus(O1 and Q3),Penicillium commune(V5-1), Penicillium rubens(MQ-5) andAspergillus aculeatus(MB1-1). There are twoPenicilliumspecies of the five OTA-producing strains and our study is the first to report thatP. rubens,T. rugulosusandA. aculeatuscan produce OTA. This work would contribute to comprehensively understanding the fungi with an OTA-producing ability in grapes before harvest and then take effective measures to prevent OTA production.
authors.affiliation: School of Food and Biological Engineering, Jiangsu University, 301 Xuefu Road, Zhenjiang 212013, China. zhangxiaoyungu@126.com.
authors.forename: Xiaoyun
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Hubei Key Laboratory, Edible Wild Plants Conservation and Utilization, 11 Cihu Road, Huangshi 435002, China. liyulin7226@163.com.
authors.forename: Yulin
authors.initials: Y
authors.lastname: Li
authors.affiliation: School of Food and Biological Engineering, Jiangsu University, 301 Xuefu Road, Zhenjiang 212013, China. wanghaiying914@163.com.
authors.forename: Haiying
authors.initials: H
authors.lastname: Wang
authors.affiliation: School of Grain Science and Technology, Jiangsu University of Science and Technology, 2 Mengxi Road, Zhenjiang 212003, China. sdxyugu@126.com.
authors.forename: Xiangyu
authors.initials: X
authors.lastname: Gu
authors.affiliation: School of Food and Biological Engineering, Jiangsu University, 301 Xuefu Road, Zhenjiang 212013, China. 18796002921@163.com.
authors.forename: Xiangfeng
authors.initials: X
authors.lastname: Zheng
authors.affiliation: School of Food and Biological Engineering, Jiangsu University, 301 Xuefu Road, Zhenjiang 212013, China. 13952967706@163.com.
authors.forename: Yun
authors.initials: Y
authors.lastname: Wang
authors.affiliation: School of Food and Biological Engineering, Jiangsu University, 301 Xuefu Road, Zhenjiang 212013, China. wenjun414727@163.com.
authors.forename: Junwei
authors.initials: J
authors.lastname: Diao
authors.affiliation: School of Food and Biological Engineering, Jiangsu University, 301 Xuefu Road, Zhenjiang 212013, China. 15751011009@163.com.
authors.forename: Yaping
authors.initials: Y
authors.lastname: Peng
authors.affiliation: School of Food and Biological Engineering, Jiangsu University, 301 Xuefu Road, Zhenjiang 212013, China. zhanghongyin126@126.com.
authors.forename: Hongyin
authors.initials: H
authors.lastname: Zhang
mesh_terms: D001230:Aspergillus / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D004271:DNA, Fungal / Q000032:analysis; D005506:Food Contamination / Q000032:analysis; D005800:Genes, Fungal; D009793:Ochratoxins / Q000096:biosynthesis*; D010407:Penicillium / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D010802:Phylogeny; D032901:Talaromyces / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D027843:Vitis / Q000382:microbiology*
chemical_list: D004271:DNA, Fungal; D009793:Ochratoxins; C025589:ochratoxin A
keywords: fungi; grapes; identification; ochratoxin A (OTA); screening