title: Integrative Approaches for the Identification and Localization of Specialized Metabolites in Tripterygium Roots.
abstract: Members of the genus Tripterygium are known to contain an astonishing diversity of specialized metabolites. The lack of authentic standards has been an impediment to the rapid identification of such metabolites in extracts. We employed an approach that involves the searching of multiple, complementary chromatographic and spectroscopic data sets against the Spektraris database to speed up the metabolite identification process. Mass spectrometry-based imaging indicated a differential localization of triterpenoids to the periderm and sesquiterpene alkaloids to the cortex layer of Tripterygium roots. We further provide evidence that triterpenoids are accumulated to high levels in cells that contain suberized cell walls, which might indicate a mechanism for storage. To our knowledge, our data provide first insights into the cell type specificity of metabolite accumulation in Tripterygium and set the stage for furthering our understanding of the biological implications of specialized metabolites in this genus.
authors.affiliation: Institute of Biological Chemistry and M.J. Murdock Metabolomics Laboratory, Washington State University, Pullman, Washington 99164-6340 (B.M.L., J.T.F., N.S., D.Š., B.C.P.); lange-m@wsu.edu.|Undergraduate Program in Biochemistry, University of Washington, Seattle, Washington 98195-7350 (M.F.L.); and lange-m@wsu.edu.|Ruđer Bošković Institute, Department of Molecular Biology, HR-10002 Zagreb, Croatia (D.Š.) lange-m@wsu.edu.
authors.forename: B Markus
authors.identifier: 0000-0001-6565-9584
authors.initials: BM
authors.lastname: Lange
authors.affiliation: Institute of Biological Chemistry and M.J. Murdock Metabolomics Laboratory, Washington State University, Pullman, Washington 99164-6340 (B.M.L., J.T.F., N.S., D.Š., B.C.P.).|Undergraduate Program in Biochemistry, University of Washington, Seattle, Washington 98195-7350 (M.F.L.); and.|Ruđer Bošković Institute, Department of Molecular Biology, HR-10002 Zagreb, Croatia (D.Š.).
authors.forename: Justin T
authors.initials: JT
authors.lastname: Fischedick
authors.affiliation: Institute of Biological Chemistry and M.J. Murdock Metabolomics Laboratory, Washington State University, Pullman, Washington 99164-6340 (B.M.L., J.T.F., N.S., D.Š., B.C.P.).|Undergraduate Program in Biochemistry, University of Washington, Seattle, Washington 98195-7350 (M.F.L.); and.|Ruđer Bošković Institute, Department of Molecular Biology, HR-10002 Zagreb, Croatia (D.Š.).
authors.forename: Malte F
authors.initials: MF
authors.lastname: Lange
authors.affiliation: Institute of Biological Chemistry and M.J. Murdock Metabolomics Laboratory, Washington State University, Pullman, Washington 99164-6340 (B.M.L., J.T.F., N.S., D.Š., B.C.P.).|Undergraduate Program in Biochemistry, University of Washington, Seattle, Washington 98195-7350 (M.F.L.); and.|Ruđer Bošković Institute, Department of Molecular Biology, HR-10002 Zagreb, Croatia (D.Š.).
authors.forename: Narayanan
authors.identifier: 0000-0001-7934-7987
authors.initials: N
authors.lastname: Srividya
authors.affiliation: Institute of Biological Chemistry and M.J. Murdock Metabolomics Laboratory, Washington State University, Pullman, Washington 99164-6340 (B.M.L., J.T.F., N.S., D.Š., B.C.P.).|Undergraduate Program in Biochemistry, University of Washington, Seattle, Washington 98195-7350 (M.F.L.); and.|Ruđer Bošković Institute, Department of Molecular Biology, HR-10002 Zagreb, Croatia (D.Š.).
authors.forename: Dunja
authors.identifier: 0000-0002-0600-6847
authors.initials: D
authors.lastname: Šamec
authors.affiliation: Institute of Biological Chemistry and M.J. Murdock Metabolomics Laboratory, Washington State University, Pullman, Washington 99164-6340 (B.M.L., J.T.F., N.S., D.Š., B.C.P.).|Undergraduate Program in Biochemistry, University of Washington, Seattle, Washington 98195-7350 (M.F.L.); and.|Ruđer Bošković Institute, Department of Molecular Biology, HR-10002 Zagreb, Croatia (D.Š.).
authors.forename: Brenton C
authors.initials: BC
authors.lastname: Poirier
mesh_terms: D000470:Alkaloids / Q000032:analysis / Q000378:metabolism; D016208:Databases, Factual; D007091:Image Processing, Computer-Assisted; D055432:Metabolomics / Q000379:methods*; D059828:Plant Cells / Q000737:chemistry / Q000378:metabolism; D010936:Plant Extracts / Q000032:analysis / Q000737:chemistry; D018517:Plant Roots / Q000737:chemistry / Q000378:metabolism*; D012717:Sesquiterpenes / Q000032:analysis / Q000378:metabolism; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D029757:Tripterygium / Q000737:chemistry / Q000166:cytology / Q000378:metabolism*
chemical_list: D000470:Alkaloids; D010936:Plant Extracts; D012717:Sesquiterpenes