title: A microRNA biomarker panel for the non-invasive detection of bladder cancer.
abstract: The development of accurate, non-invasive urinary assays for bladder cancer would greatly facilitate the detection and management of a disease that has a high rate of recurrence and progression. In this study, we employed a discovery and validation strategy to identify microRNA signatures that can perform as a non-invasive bladder cancer diagnostic assay. Expression profiling of 754 human microRNAs (TaqMan low density arrays) was performed on naturally voided urine samples from a cohort of 85 subjects of known bladder disease status (27 with active BCa). A panel of 46 microRNAs significantly associated with bladder cancer were subsequently monitored in an independent cohort of 121 subjects (61 with active BCa) using quantitative real-time PCR (RT-PCR). Multivariable modeling identified a 25-target diagnostic signature that predicted the presence of BCa with an estimated sensitivity of 87% at a specificity of 100% (AUC 0.982). With additional validation, the monitoring of a urinary microRNA biomarker panel could facilitate the non-invasive evaluation of patients under investigation for BCa.
authors.affiliation: Nonagen Bioscience Corporation, Jacksonville, FL, USA.
authors.forename: Virginia
authors.initials: V
authors.lastname: Urquidi
authors.affiliation: Cancer Research Institute, MD Anderson Cancer Center, Orlando, FL, USA.
authors.forename: Mandy
authors.initials: M
authors.lastname: Netherton
authors.affiliation: Cancer Research Institute, MD Anderson Cancer Center, Orlando, FL, USA.
authors.forename: Evan
authors.initials: E
authors.lastname: Gomes-Giacoia
authors.affiliation: Department of Health Sciences Research, Mayo Clinic, Jacksonville, FL USA.
authors.forename: Daniel J
authors.initials: DJ
authors.lastname: Serie
authors.affiliation: Department of Health Sciences Research, Mayo Clinic, Rochester, MN USA.
authors.forename: Jeanette
authors.initials: J
authors.lastname: Eckel-Passow
authors.affiliation: Nonagen Bioscience Corporation, Jacksonville, FL, USA.|University of Hawaii Cancer Center, Honolulu, HI USA.
authors.forename: Charles J
authors.initials: CJ
authors.lastname: Rosser
authors.affiliation: Nonagen Bioscience Corporation, Jacksonville, FL, USA.|Department of Health Sciences Research, Mayo Clinic, Jacksonville, FL USA.|Department of Urology, Mayo Clinic, Jacksonville, FL USA.
authors.forename: Steve
authors.initials: S
authors.lastname: Goodison
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000652:urine*; D005260:Female; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000652:urine*; D008875:Middle Aged; D060888:Real-Time Polymerase Chain Reaction; D001749:Urinary Bladder Neoplasms / Q000175:diagnosis* / Q000652:urine
chemical_list: D014408:Biomarkers, Tumor; D035683:MicroRNAs
keywords: bladder cancer; diagnostic biomarkers; microRNA; multiplex; urinalysis