title: Efficient continuous biosynthesis of silver nanoparticles by activated sludge micromycetes with enhanced tolerance to metal ion toxicity.
abstract: The method for producing AgNPs by granules of activated sludge micromycetes with enhanced tolerance to metal ion toxicity - Penicillium glabrum, Fusarium nivale and Fusarium oxysporum has been developed; the optimum conditions for AgNP biosynthesis being found: the Ag+ion concentration, duration of the contact of microbial cells with silver ions, a growth phase of microorganisms, medium composition, a рН value, mixing conditions, and also lighting intensity. The effect of Cl-, SO42-and HPO42-ions binding Ag+ions was eliminated, that brought to significant increase of the yield of NPs. Under batch conditions, silver particles of 60-110 nanometers in size were formed with a 65% yield. It was established that the nanoparticles were covered with microbial cell membrane proteins composed up to 70% by weight of the NPs that prevented their aggregation. In addition, it was the first time stable AgNPs had been formed by continuous AgNP biosynthesis by living cells of F. oxysporum with an 80% yield for a long time.
authors.affiliation: Department of Biotechnology, Faculty of Biotechnology and Industrial Ecology, D. Mendeleyev University of Chemical Technology of Russia, 9 Miusskaya sq., Moscow 125047, Russia.
authors.forename: Dmitry V
authors.initials: DV
authors.lastname: Tyupa
authors.affiliation: Department of Biotechnology, Faculty of Biotechnology and Industrial Ecology, D. Mendeleyev University of Chemical Technology of Russia, 9 Miusskaya sq., Moscow 125047, Russia. Electronic address: wsezart@yandex.ru.
authors.forename: Sergei V
authors.initials: SV
authors.lastname: Kalenov
authors.affiliation: Department of Biotechnology, Faculty of Biotechnology and Industrial Ecology, D. Mendeleyev University of Chemical Technology of Russia, 9 Miusskaya sq., Moscow 125047, Russia.
authors.forename: Marina M
authors.initials: MM
authors.lastname: Baurina
authors.affiliation: Department of Analytical, Physical and Colloid Chemistry, Faculty of Pharmacy, I. Sechenov First Moscow State Medical University, 2-4 Bolshaya Pirogovskaya st., Moscow 119991, Russia.
authors.forename: Liubov M
authors.initials: LM
authors.lastname: Yakubovich
authors.affiliation: Department of Technology of Inorganic Substances and Electrochemical Processes, Faculty of Technology of Inorganic Substances and High Temperature Materials, D. Mendeleyev University of Chemical Technology of Russia, 9 Miusskaya sq., Moscow 125047, Russia.
authors.forename: Alexander N
authors.initials: AN
authors.lastname: Morozov
authors.affiliation: Laboratory of High-Temperature Solution Crystallization, Department of Crystalline Material Growth, A. Shubnikov Institute of Crystallography, Russian Academy of Sciences, 59 Leninskii pr., Moscow 117333, Russia.
authors.forename: Ruslan M
authors.initials: RM
authors.lastname: Zakalyukin
authors.affiliation: S. Winogradsky Institute of Microbiology, Research Center of Biotechnology, Russian Academy of Sciences, 7 build. 2 60-letiya Oktyabrya pr., Moscow 117312, Russia.
authors.forename: Vladimir V
authors.initials: VV
authors.lastname: Sorokin
authors.affiliation: S. Winogradsky Institute of Microbiology, Research Center of Biotechnology, Russian Academy of Sciences, 7 build. 2 60-letiya Oktyabrya pr., Moscow 117312, Russia.
authors.forename: Dmitry A
authors.initials: DA
authors.lastname: Skladnev
mesh_terms: D018533:Biomass; D001709:Biotechnology; D003470:Culture Media; D005670:Fusarium / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*; D055772:Green Chemistry Technology; D006863:Hydrogen-Ion Concentration; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000633:toxicity / Q000648:ultrastructure; D036103:Nanotechnology; D010407:Penicillium / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*; D012722:Sewage / Q000382:microbiology*; D012834:Silver / Q000737:chemistry* / Q000633:toxicity
chemical_list: D003470:Culture Media; D012722:Sewage; D012834:Silver
keywords: Biosynthesis of nanosilver; NP green biosynthesis; Nanosilver; Nanotechnology; Silver nanoparticles