title: Quantifying the growth of chlamydia suis in cell culture using high-content microscopy.
abstract: The porcine pathogen Chlamydia suis is widespread in pig farming. Isolation of Chlamydia suis in cell culture is crucial for the generation and characterization of new isolates. However, isolation of Chlamydia suis strains from field samples is fastidious. Therefore, we exploited high-content microscopy to quantify the growth of Chlamydia suis strains in different cell lines. We found that the cell line yielding optimal propagation of Chlamydia suis differed among isolates, and we identified cell lines outperforming those routinely used for chlamydial isolation. We conclude that adaptation of the propagation procedure to the origin of the putative field isolate is highly recommended to improve the recovery rate.
authors.affiliation: Lab of Immunology and Animal Biotechnology, Faculty of Bioscience Engineering, University of Ghent, Ghent, Belgium.
authors.forename: Leentje
authors.initials: L
authors.lastname: De Puysseleyr
authors.affiliation: Lab of Immunology and Animal Biotechnology, Faculty of Bioscience Engineering, University of Ghent, Ghent, Belgium.
authors.forename: Kristien
authors.initials: K
authors.lastname: De Puysseleyr
authors.affiliation: Lab of Immunology and Animal Biotechnology, Faculty of Bioscience Engineering, University of Ghent, Ghent, Belgium.
authors.forename: Daisy
authors.initials: D
authors.lastname: Vanrompay
authors.affiliation: Lab of Cell Biology and Histology, Faculty of Veterinary Sciences, University of Antwerp, Antwerp, Belgium.|Cell Systems and Imaging, Faculty of Bioscience Engineering, University of Ghent, Ghent, Belgium.
authors.forename: Winnok H
authors.initials: WH
authors.lastname: De Vos
mesh_terms: D000818:Animals; D018938:Caco-2 Cells; D045744:Cell Line, Tumor; D002689:Chlamydia / Q000254:growth & development* / Q000302:isolation & purification; D002690:Chlamydia Infections / Q000382:microbiology / Q000662:veterinary*; D002522:Chlorocebus aethiops; D006801:Humans; D051379:Mice; D008853:Microscopy; D013552:Swine; D013553:Swine Diseases / Q000382:microbiology; D014709:Vero Cells
keywords: Chlamydia; cell culture; high-content microscopy; isolation; swine