title: Tips and Tricks for Exogenous Application of Synthetic Post-translationally Modified Peptides to Plants.
abstract: The first signaling peptide discovered and purified was insulin in 1921. However, it was not until 1991 that the first peptide signal, systemin, was discovered in plants. Since the discovery of systemin, peptides have emerged as a potent and diverse class of signaling molecules in plant systems. Peptides consist of small amino acid sequences, which often act as ligands of receptor kinases. However, not all peptides are created equal, and signaling peptides are grouped into several subgroups dependent on the type of post-translational processing they undergo. Here, we focus on the application of synthetic, post-translationally modified peptides (PTMPs) to plant systems, describing several methods appropriate for the use of peptides in Arabidopsis thaliana and crop models.
authors.affiliation: Division of Plant and Crop Sciences, School of Biosciences, University of Nottingham, Loughborough, LE12 5RD, UK.
authors.forename: Nathan
authors.initials: N
authors.lastname: Czyzewicz
authors.affiliation: Department of Plant Systems Biology, Flemish Institute of Biotechnology (VIB), 9052, Ghent, Belgium.|Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052, Ghent, Belgium.|Medical Biotechnology Center, Flemish Institute of Biotechnology VIB, 9000, Ghent, Belgium.|Department of Biochemistry, Ghent University, 9000, Ghent, Belgium.
authors.forename: Elisabeth
authors.initials: E
authors.lastname: Stes
authors.affiliation: Division of Plant and Crop Sciences, School of Biosciences, University of Nottingham, Loughborough, LE12 5RD, UK. ive.desmet@psb.vib-ugent.be.|Department of Plant Systems Biology, Flemish Institute of Biotechnology (VIB), 9052, Ghent, Belgium. ive.desmet@psb.vib-ugent.be.|Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052, Ghent, Belgium. ive.desmet@psb.vib-ugent.be.|Centre for Plant Integrative Biology, University of Nottingham, Loughborough, LE12 5RD, UK. ive.desmet@psb.vib-ugent.be.
authors.forename: Ive
authors.initials: I
authors.lastname: De Smet
mesh_terms: D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000235:genetics*; D008024:Ligands; D010940:Plant Proteins / Q000235:genetics*; D011499:Protein Processing, Post-Translational / Q000235:genetics*; D021382:Protein Sorting Signals / Q000235:genetics*; D015398:Signal Transduction / Q000235:genetics
chemical_list: D008024:Ligands; D010940:Plant Proteins; D021382:Protein Sorting Signals
keywords: Arabidopsis; Cereal crops; In vitro growth; Post-translationally modified peptide; Synthetic peptide