title: Protection of resveratrol on acute kidney injury in septic rats.
abstract: OBJECTIVE
  The aim of the study is to investigate protective effect of resveratrol (Res) on acute kidney injury (AKI) in sepsis.
  
  
  METHODS
  Rats in sham group received sham operation; in sham + Res received sham operation and Res (3 mg/kg); in cecal ligation and puncture (CLP) established as sepsis; in CLP + Res (3 mg/kg) with sepsis and Res (3 mg/kg); and in CLP + Res (10 mg/kg) with sepsis and Res (10 mg/kg). Survival rate, serum indexes, inflammatory factors, NF-κB-P65, and SIRT1 were detected. Lipopolysaccharide (LPS) mesangial cell was with Res and SIRT1 silencing.
  
  
  RESULTS
  (1) Res intervention improved survival rate of CLP rat. (2) Compared to sham, serum creatinine, blood urine nitrogen, serum cystatin C, neutrophil gelatinase-associated lipocalin, kidney injury molecule-1, tumor necrosis factor-α, interleukin-1β, IL-6, and renal injury index increased in CLP group, while decreased in CLP + Res (3 mg/kg) and CLP + Res (10 mg/kg), significantly, as dose-dependent ( p < 0.05). (3) With Res, NF-κB-P65 and de-acetylated SIRT1 decreased, while SIRT1 and de-acetylated Nuclear factor kB-p65 9 NF-κB-P65) increased, significantly ( p < 0.05). (4) SIRT1 and de-acetylated NF-κB-P65 decreased in LPS cells, while SIRT1 increased after Res intervention, significantly ( p < 0.05). After silencing SIRT1, de-acetylated NF-κB-P65 increased, significantly ( p < 0.05).
  
  
  CONCLUSIONS
  Res increases the survival rate of septic rats by inhibiting inflammatory factors to ease AKI and promotes NF-κB-P65 de-acetylation by upregulating SIRT1.
authors.affiliation: 1 Department of Pediatrics, Zhujiang Hospital of Southern Medical University, Guangzhou, Guangdong, China.|2 Department of Pediatrics, The First Hospital of Huhehaote, The Inner Mongolia Autonomous Region, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Gan
authors.affiliation: 1 Department of Pediatrics, Zhujiang Hospital of Southern Medical University, Guangzhou, Guangdong, China.
authors.forename: S
authors.initials: S
authors.lastname: Tao
authors.affiliation: 3 Department of Pediatrics, Shenzhen Children's Hospital, Shenzhen, Guangdong, China.
authors.forename: D
authors.initials: D
authors.lastname: Cao
authors.affiliation: 1 Department of Pediatrics, Zhujiang Hospital of Southern Medical University, Guangzhou, Guangdong, China.
authors.forename: H
authors.initials: H
authors.lastname: Xie
authors.affiliation: 1 Department of Pediatrics, Zhujiang Hospital of Southern Medical University, Guangzhou, Guangdong, China.
authors.forename: Q
authors.initials: Q
authors.lastname: Zeng
mesh_terms: D058186:Acute Kidney Injury / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology / Q000517:prevention & control*; D000818:Animals; D016207:Cytokines / Q000097:blood; D005919:Glomerular Filtration Rate / Q000187:drug effects; D007668:Kidney / Q000187:drug effects / Q000378:metabolism / Q000473:pathology / Q000502:physiology; D008297:Male; D020011:Protective Agents / Q000494:pharmacology / Q000627:therapeutic use*; D017207:Rats, Sprague-Dawley; D012079:Renal Circulation / Q000187:drug effects; D000077185:Resveratrol; D018805:Sepsis / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology; D056564:Sirtuin 1 / Q000378:metabolism; D013267:Stilbenes / Q000494:pharmacology / Q000627:therapeutic use*; D051996:Transcription Factor RelA / Q000378:metabolism
chemical_list: D016207:Cytokines; D020011:Protective Agents; D013267:Stilbenes; D051996:Transcription Factor RelA; C488368:Sirt1 protein, rat; D056564:Sirtuin 1; D000077185:Resveratrol
keywords: NF-κB-P65; Resveratrol; SIRT1; acute kidney injury; sepsis