title: Functionalized nanoparticles enable tracking the rapid entry and release of doxorubicin in human pancreatic cancer cells.
abstract: Efficient drug delivery is critical to therapy. Using electron microscopy, X-ray, and light microscopy, we have characterized functionalized superparamagnetic iron oxide (SPIO) nanoparticles, and determined their ability for rapid entry and release of the cancer drug doxorubicin in human pancreatic cancer cells. Dextran-coated SPIO nanoparticle ferrofluid, functionalized with the red-autofluorescing doxorubicin and the green-fluorescent dye fluorescein isothiocyanate as a reporter, enables tracking the intracellular nanoparticle transport and drug release. This engineered nanoparticle enables a >20 fold rapid entry and release of the drug in human pancreatic cancer cells, holding therapeutic potential as an advanced drug delivery and imaging platform. The low extracellular pH of most tumors precluding the entry of a number of weakly basic drugs such as doxorubicin, conferring drug resistance, can now be overcome.
authors.affiliation: Department of Physics & Astronomy, Wayne State University, Detroit, MI 48201, USA.
authors.forename: Maheshika P
authors.initials: MP
authors.lastname: Arachchige
authors.affiliation: Department of Physiology, Wayne State University School of Medicine, Detroit, MI 48201, USA.
authors.forename: Suvra S
authors.initials: SS
authors.lastname: Laha
authors.affiliation: Department of Physiology, Wayne State University School of Medicine, Detroit, MI 48201, USA.
authors.forename: Akshata R
authors.initials: AR
authors.lastname: Naik
authors.affiliation: Department of Physiology, Wayne State University School of Medicine, Detroit, MI 48201, USA.
authors.forename: Kenneth T
authors.initials: KT
authors.lastname: Lewis
authors.affiliation: Department of Physics & Astronomy, Wayne State University, Detroit, MI 48201, USA.
authors.forename: Ratna
authors.initials: R
authors.lastname: Naik
authors.affiliation: Department of Physics & Astronomy, Wayne State University, Detroit, MI 48201, USA; Department of Physiology, Wayne State University School of Medicine, Detroit, MI 48201, USA. Electronic address: bjena@med.wayne.edu.
authors.forename: Bhanu P
authors.initials: BP
authors.lastname: Jena
mesh_terms: D000903:Antibiotics, Antineoplastic / Q000008:administration & dosage* / Q000378:metabolism; D045744:Cell Line, Tumor; D004317:Doxorubicin / Q000008:administration & dosage* / Q000378:metabolism; D016503:Drug Delivery Systems / Q000379:methods*; D005290:Ferric Compounds / Q000737:chemistry; D016650:Fluorescein-5-isothiocyanate / Q000737:chemistry / Q000378:metabolism; D005453:Fluorescence; D006801:Humans; D058185:Magnetite Nanoparticles / Q000737:chemistry* / Q000706:statistics & numerical data; D053758:Nanoparticles / Q000737:chemistry / Q000378:metabolism*; D010190:Pancreatic Neoplasms / Q000188:drug therapy
chemical_list: D000903:Antibiotics, Antineoplastic; D005290:Ferric Compounds; D058185:Magnetite Nanoparticles; C000499:ferric oxide; D004317:Doxorubicin; D016650:Fluorescein-5-isothiocyanate
keywords: Doxorubicin; Drug delivery; Pancreatic cancer; SPIO nano particles