title: Global transcriptomic response of Anoxybacillus sp. SK 3-4 to aluminum exposure.
abstract: Anoxybacillus sp. SK 3-4 is a Gram-positive, rod-shaped bacterium and a member of family Bacillaceae. We had previously reported that the strain is an aluminum resistant thermophilic bacterium. This is the first report to provide a detailed analysis of the global transcriptional response of Anoxybacillus when the cells were exposed to 600 mg L-1of aluminum. The transcriptome was sequenced using Illumina MiSeq sequencer. Total of 708 genes were differentially expressed (fold change >2.00) with 316 genes were up-regulated while 347 genes were down-regulated, in comparing to control with no aluminum added in the culture. Based on Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis, the majority of genes encoding for cell metabolism such as glycolysis, sulfur metabolism, cysteine and methionine metabolism were up-regulated; while most of the gene associated with tricarboxylic acid cycle (TCA cycle) and valine, leucine and isoleucine metabolism were down-regulated. In addition, a significant number of the genes encoding ABC transporters, metal ions transporters, and some stress response proteins were also differentially expressed following aluminum exposure. The findings provide further insight and help us to understand on the resistance of Anoxybacillus sp. SK 3-4 toward aluminium.
authors.affiliation: Faculty of Biosciences and Medical Engineering, Universiti Teknologi Malaysia, Skudai, Johor, Malaysia.
authors.forename: Jia Chun
authors.initials: JC
authors.lastname: Lim
authors.affiliation: Faculty of Biosciences and Medical Engineering, Universiti Teknologi Malaysia, Skudai, Johor, Malaysia.
authors.forename: Suganthi
authors.initials: S
authors.lastname: Thevarajoo
authors.affiliation: Faculty of Biosciences and Medical Engineering, Universiti Teknologi Malaysia, Skudai, Johor, Malaysia.
authors.forename: Chitra
authors.initials: C
authors.lastname: Selvaratnam
authors.affiliation: Faculty of Biosciences and Medical Engineering, Universiti Teknologi Malaysia, Skudai, Johor, Malaysia.
authors.forename: Kian Mau
authors.initials: KM
authors.lastname: Goh
authors.affiliation: Faculty of Biosciences and Medical Engineering, Universiti Teknologi Malaysia, Skudai, Johor, Malaysia.
authors.forename: Mohd Shahir
authors.initials: MS
authors.lastname: Shamsir
authors.affiliation: Faculty of Biosciences and Medical Engineering, Universiti Teknologi Malaysia, Skudai, Johor, Malaysia.
authors.forename: Zaharah
authors.initials: Z
authors.lastname: Ibrahim
authors.affiliation: Faculty of Biosciences and Medical Engineering, Universiti Teknologi Malaysia, Skudai, Johor, Malaysia.
authors.forename: Chun Shiong
authors.initials: CS
authors.lastname: Chong
mesh_terms: D000535:Aluminum / Q000378:metabolism*; D058677:Anoxybacillus / Q000187:drug effects* / Q000235:genetics*; D020869:Gene Expression Profiling; D059014:High-Throughput Nucleotide Sequencing; D053858:Metabolic Networks and Pathways / Q000235:genetics; D017422:Sequence Analysis, DNA; D013312:Stress, Physiological
chemical_list: D000535:Aluminum
keywords: Aluminum; Anoxybacillus sp. SK 3-4; Transcriptomic