title: Microfluidic Tissue Mesodissection in Molecular Cancer Diagnostics.
abstract: We present a mesodissection platform that retains the advantages of laser-based dissection instrumentation with the speed and ease of manual dissection. Tissue dissection in clinical laboratories is often performed by manually scraping a physician-selected region from standard glass slide mounts. In this manner, costs associated with dissection remain low, but spatial resolution is compromised. In contrast, laser microdissection methods maintain spatial resolution that matches the requirements for analysis of important tissue heterogeneity but remains costly and labor intensive. We demonstrate a microfluidic tool for rapid extraction of histological regions of interest from formalin-fixed paraffin-embedded tissue, which uses a simple and automated method that is compatible with most downstream enzymatic reactions, including protocols used for next-generation DNA sequencing.
authors.affiliation: 1 Electronics Organization, GE Global Research Center, Niskayuna, NY, USA.
authors.forename: Christine
authors.initials: C
authors.lastname: Surrette
authors.affiliation: 2 Diagnostics and Biomedical Technologies, GE Global Research Center, Niskayuna, NY, USA.
authors.forename: David
authors.initials: D
authors.lastname: Shoudy
authors.affiliation: 1 Electronics Organization, GE Global Research Center, Niskayuna, NY, USA.
authors.forename: Alex
authors.initials: A
authors.lastname: Corwin
authors.affiliation: 2 Diagnostics and Biomedical Technologies, GE Global Research Center, Niskayuna, NY, USA.
authors.forename: Wei
authors.initials: W
authors.lastname: Gao
authors.affiliation: 2 Diagnostics and Biomedical Technologies, GE Global Research Center, Niskayuna, NY, USA.
authors.forename: Maria I
authors.initials: MI
authors.lastname: Zavodszky
authors.affiliation: 3 NeuroInDx, Inc., Torrance, CA, USA.
authors.forename: Stanislav L
authors.initials: SL
authors.lastname: Karsten
authors.affiliation: 1 Electronics Organization, GE Global Research Center, Niskayuna, NY, USA.
authors.forename: Todd
authors.initials: T
authors.lastname: Miller
authors.affiliation: 2 Diagnostics and Biomedical Technologies, GE Global Research Center, Niskayuna, NY, USA.
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Gerdes
authors.affiliation: 2 Diagnostics and Biomedical Technologies, GE Global Research Center, Niskayuna, NY, USA.
authors.forename: Nichole
authors.initials: N
authors.lastname: Wood
authors.affiliation: 2 Diagnostics and Biomedical Technologies, GE Global Research Center, Niskayuna, NY, USA.
authors.forename: John R
authors.initials: JR
authors.lastname: Nelson
authors.affiliation: 1 Electronics Organization, GE Global Research Center, Niskayuna, NY, USA.
authors.forename: Chris M
authors.initials: CM
authors.lastname: Puleo
mesh_terms: D057205:Automation, Laboratory; D004210:Dissection / Q000295:instrumentation / Q000379:methods*; D006801:Humans; D044085:Microfluidics / Q000295:instrumentation / Q000379:methods*; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D009369:Neoplasms / Q000175:diagnosis*; D057089:Pathology, Molecular / Q000295:instrumentation / Q000379:methods*
keywords: and genomics; clinical automation; microfluidics; molecular biology; robotics and instrumentation