title: Comparative expression profile of NOD1/2 and certain acute inflammatory cytokines in thermal-stressed cell culture model of native and crossbred cattle.
abstract: Thermotolerance depends mainly on the health and immune status of the animals. The variation in the immune status of the animals may alter the level of tolerance of animals exposed to heat or cold stress. The present study was conducted to investigate the expression profile of two important nucleotide binding and oligomerization domain receptors (NLRs) (NOD1 and NOD2) and their central signalling molecule RIP2 gene during in vitro thermal-stressed bovine peripheral blood mononuclear cells (PBMCs) of native (Sahiwal) and crossbred (Sahiwal X HF) cattle. We also examined the differential expression profile of certain acute inflammatory cytokines in in vitro thermal-stressed PBMC culture among native and its crossbred counterparts. Results revealed that the expression profile of NOD1/2 positively correlates with the thermal stress, signalling molecule and cytokines. Present findings also highlighted that the expression patterns during thermal stress were comparatively superior among indigenous compared to crossbred cattle which may add references regarding the better immune adaptability of Zebu cattle.
authors.affiliation: Molecular Genetics Laboratory, ICAR-Central Institute for Research on Cattle, Meerut, Uttar Pradesh, 250001, India. bhanupvhn24@gmail.com.
authors.forename: V
authors.initials: V
authors.lastname: Bhanuprakash
authors.affiliation: Molecular Genetics Laboratory, ICAR-Central Institute for Research on Cattle, Meerut, Uttar Pradesh, 250001, India.
authors.forename: Umesh
authors.initials: U
authors.lastname: Singh
authors.affiliation: Molecular Genetics Laboratory, ICAR-Central Institute for Research on Cattle, Meerut, Uttar Pradesh, 250001, India.
authors.forename: Gyanendra Singh
authors.initials: GS
authors.lastname: Sengar
authors.affiliation: Molecular Genetics Laboratory, ICAR-Central Institute for Research on Cattle, Meerut, Uttar Pradesh, 250001, India.
authors.forename: T V
authors.initials: TV
authors.lastname: Raja
authors.affiliation: School of Abiotic Stress Management, ICAR-National Institute of Abiotic Stress Management, Baramati, India.
authors.forename: Basavraj
authors.initials: B
authors.lastname: Sajjanar
authors.affiliation: Molecular Genetics Laboratory, ICAR-Central Institute for Research on Cattle, Meerut, Uttar Pradesh, 250001, India.
authors.forename: Rani
authors.initials: R
authors.lastname: Alex
authors.affiliation: Molecular Genetics Laboratory, ICAR-Central Institute for Research on Cattle, Meerut, Uttar Pradesh, 250001, India.
authors.forename: Sushil
authors.initials: S
authors.lastname: Kumar
authors.affiliation: Molecular Genetics Laboratory, ICAR-Central Institute for Research on Cattle, Meerut, Uttar Pradesh, 250001, India.
authors.forename: R R
authors.initials: RR
authors.lastname: Alyethodi
authors.affiliation: Molecular Genetics Laboratory, ICAR-Central Institute for Research on Cattle, Meerut, Uttar Pradesh, 250001, India.
authors.forename: Ashish
authors.initials: A
authors.lastname: Kumar
authors.affiliation: Molecular Genetics Laboratory, ICAR-Central Institute for Research on Cattle, Meerut, Uttar Pradesh, 250001, India.
authors.forename: Ankur
authors.initials: A
authors.lastname: Sharma
authors.affiliation: Molecular Genetics Laboratory, ICAR-Central Institute for Research on Cattle, Meerut, Uttar Pradesh, 250001, India.
authors.forename: Suresh
authors.initials: S
authors.lastname: Kumar
authors.affiliation: Division of Animal Genetics and Breeding, ICAR-Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh, 243122, India.
authors.forename: Bharat
authors.initials: B
authors.lastname: Bhusan
authors.affiliation: Molecular Genetics Laboratory, ICAR-Central Institute for Research on Cattle, Meerut, Uttar Pradesh, 250001, India. drrajibdeb@gmail.com.
authors.forename: Rajib
authors.initials: R
authors.lastname: Deb
mesh_terms: D000818:Animals; D002417:Cattle; D002478:Cells, Cultured; D058639:Cold-Shock Response / Q000235:genetics; D016207:Cytokines / Q000235:genetics*; D005260:Female; D015870:Gene Expression; D018869:Heat-Shock Response / Q000235:genetics*; D007963:Leukocytes, Mononuclear / Q000378:metabolism*; D053474:Nod1 Signaling Adaptor Protein / Q000235:genetics*; D053473:Nod2 Signaling Adaptor Protein / Q000235:genetics*; D012333:RNA, Messenger / Q000378:metabolism; D053475:Receptor-Interacting Protein Serine-Threonine Kinase 2 / Q000235:genetics*; D013045:Species Specificity
chemical_list: D016207:Cytokines; D053474:Nod1 Signaling Adaptor Protein; D053473:Nod2 Signaling Adaptor Protein; D012333:RNA, Messenger; D053475:Receptor-Interacting Protein Serine-Threonine Kinase 2
keywords: Cattle; IL-1; IL-6; IL-8; NOD1; NOD2; RIP2; TNFα; Thermal stress