title: MR targeted imaging for the expression of tenascin-C in myocardial infarction in vivo.
abstract: To investigate the presence of viable myocardium in mice with acute myocardial infarction (MI) using a molecular targeted probe.
  
  
  
  Super paramagnetic iron oxide (SPIO) nanoparticles and tenascin-C antibody were conjugated as an MRI probe. Fifteen mice with infarction were injected with SPIO-anti-tenascin-C (3 days [d], 5d, 7d after infarction; n = 5 for each group). Another five mice with infarction (5d, n = 5) were injected with SPIO for comparison. In vivo MR (7 Tesla, fast low-angle shot multi-slice T2* sequence) was performed for tracing. Histological analysis was used to compare surviving cardiomyocytes with signal changes on MR.
  
  
  
  The mRNA expression of tenascin-C increased directly after MI and peaked at the fifth day (5d 24.29 ± 1.41 versus 3d 10.63 ± 0.72, 7d 6.56 ± 0.12; P < 0.01). T2relaxation rate of synthesized SPIO-anti-tenascin-C was r2 = 338 mM-1s-1. After MR, the signal changes (contrast-to-noise ratio) of the research group were 3d 6.51 ± 1.13 versus 5d 14.06 ± 3.19 versus 7d 5.02 ± 2.65, P < 0.05. The MR signal showed a small decrease in the contrast group on 5d (research group 14.06 ± 3.19 versus contrast group 1.75 ± 0.59, P < 0.05).
  
  
  
  Tenascin-C was expressed by surviving cardiomyocytes within the infarcted region. MR imaging with SPIO-anti-tenascin-C might be used to evaluate myocardial viability of MI patients before therapy.
  
  
  
  1 Technical Efficacy: Stage 4 J. MAGN. RESON. IMAGING 2017;45:1668-1674.
authors.affiliation: Department of Radiology, the First Affiliated Hospital of Nanjing Medical University, Nanjing, China.
authors.forename: Jiacheng
authors.initials: J
authors.lastname: Song
authors.affiliation: Department of Radiology, the First Affiliated Hospital of Nanjing Medical University, Nanjing, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Yu
authors.affiliation: Department of Radiology, the First Affiliated Hospital of Nanjing Medical University, Nanjing, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Li
authors.affiliation: Department of Radiology, the First Affiliated Hospital of Nanjing Medical University, Nanjing, China.
authors.forename: Shanshan
authors.initials: S
authors.lastname: Lu
authors.affiliation: Department of Radiology, the First Affiliated Hospital of Nanjing Medical University, Nanjing, China.
authors.forename: Zhanlong
authors.initials: Z
authors.lastname: Ma
authors.affiliation: Department of Radiology, the First Affiliated Hospital of Nanjing Medical University, Nanjing, China.
authors.forename: Haibin
authors.initials: H
authors.lastname: Shi
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000493:pharmacokinetics; D015415:Biomarkers / Q000378:metabolism; D003287:Contrast Media / Q000493:pharmacokinetics; D003911:Dextrans / Q000493:pharmacokinetics; D005260:Female; D008279:Magnetic Resonance Imaging / Q000379:methods*; D058185:Magnetite Nanoparticles; D051379:Mice; D008813:Mice, Inbred ICR; D057054:Molecular Imaging / Q000379:methods*; D009203:Myocardial Infarction / Q000150:complications / Q000000981:diagnostic imaging* / Q000378:metabolism*; D017682:Myocardial Stunning / Q000000981:diagnostic imaging* / Q000209:etiology / Q000378:metabolism*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D019063:Tenascin / Q000378:metabolism*
chemical_list: D000911:Antibodies, Monoclonal; D015415:Biomarkers; D003287:Contrast Media; D003911:Dextrans; D058185:Magnetite Nanoparticles; D019063:Tenascin; C097921:ferumoxtran-10
keywords: SPIO; Tenascin-C; magnetic resonance image; myocardial infarction