title: N-Glycosylation at Asn 402 Stabilizes N-Cadherin and Promotes Cell-Cell Adhesion of Glioma Cells.
abstract: Cadherin is crucial for cell-cell adhesion and N-glycosylation of N-cadherin has been implicated in the process of mammary, renal, and ovarian carcinogenesis. However, whether N-glycosylation of N-cadherin plays a role in glioma remains unknown. Previous studies had indicated that N-glycosylation could occur at three asparagine residues of N-cadherin. By generating and over-expressing N-glycosylation-deficient N-cadherin mutants in the human glioma cell lines SHG66 and U87, we found that mutation of N402 but not of the other potentially N-glycosylated residues destabilized N-cadherin and led to its ubiquitylation and subsequent proteasomal degradation. Furthermore, destabilized N-cadherin inhibited cadherin-mediated cell-cell adhesion and promoted cell migration. Our findings reveal that N-glycosylation controls N-cadherin stability and plays a role in glioma migration. J. Cell. Biochem. 118: 1423-1431, 2017. © 2016 Wiley Periodicals, Inc.
authors.affiliation: Central Hospital of Minhang District, Fudan University, Shanghai, China.|Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Fudan University, Shanghai, China.|Key Laboratory of Glycoconjugate Research, Ministry of Health, Shanghai, China.
authors.forename: Yaolin
authors.initials: Y
authors.lastname: Xu
authors.affiliation: Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Fudan University, Shanghai, China.|Key Laboratory of Glycoconjugate Research, Ministry of Health, Shanghai, China.
authors.forename: Ruiqi
authors.initials: R
authors.lastname: Chang
authors.affiliation: Central Hospital of Minhang District, Fudan University, Shanghai, China.
authors.forename: Fulin
authors.initials: F
authors.lastname: Xu
authors.affiliation: Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Fudan University, Shanghai, China.|Key Laboratory of Glycoconjugate Research, Ministry of Health, Shanghai, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Gao
authors.affiliation: Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Fudan University, Shanghai, China.|Key Laboratory of Glycoconjugate Research, Ministry of Health, Shanghai, China.
authors.forename: Fuming
authors.initials: F
authors.lastname: Yang
authors.affiliation: Shanghai Institute for Food and Drug Control, Shanghai, China.
authors.forename: Can
authors.initials: C
authors.lastname: Wang
authors.affiliation: Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Fudan University, Shanghai, China.|Key Laboratory of Glycoconjugate Research, Ministry of Health, Shanghai, China.
authors.forename: Jin
authors.initials: J
authors.lastname: Xiao
authors.affiliation: Central Hospital of Minhang District, Fudan University, Shanghai, China.
authors.forename: Zuopeng
authors.initials: Z
authors.lastname: Su
authors.affiliation: Central Hospital of Minhang District, Fudan University, Shanghai, China.
authors.forename: Yongyan
authors.initials: Y
authors.lastname: Bi
authors.affiliation: Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Fudan University, Shanghai, China.|Key Laboratory of Glycoconjugate Research, Ministry of Health, Shanghai, China.|Key Laboratory of Molecular Medicine, Ministry of Education, Shanghai, China.
authors.forename: Liying
authors.initials: L
authors.lastname: Wang
authors.affiliation: Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Fudan University, Shanghai, China.|Key Laboratory of Glycoconjugate Research, Ministry of Health, Shanghai, China.|Key Laboratory of Molecular Medicine, Ministry of Education, Shanghai, China.
authors.forename: Xiliang
authors.initials: X
authors.lastname: Zha
mesh_terms: D015703:Antigens, CD / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D001216:Asparagine / Q000737:chemistry* / Q000235:genetics; D015820:Cadherins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D002448:Cell Adhesion; D045744:Cell Line, Tumor; D002465:Cell Movement; D005910:Glioma / Q000235:genetics / Q000378:metabolism*; D006031:Glycosylation; D006801:Humans; D009154:Mutation; D017351:N-Acetylglucosaminyltransferases / Q000378:metabolism; D055550:Protein Stability
chemical_list: D015703:Antigens, CD; C050361:CDH2 protein, human; D015820:Cadherins; D001216:Asparagine; D017351:N-Acetylglucosaminyltransferases
keywords: CELL ADHESION; CELL MIGRATION; GLIOMA; N-CADHERIN; N-GLYCOSYLATION