title: Simulated ischemia/reperfusion-induced p65-Beclin 1-dependent autophagic cell death in human umbilical vein endothelial cells.
abstract: Myocardial ischemia/reperfusion (I/R) injury detrimentally alters the prognosis of patients undergoing revascularization after acute myocardial infarction. Our previous study demonstrated that NF-κB-induced autophagy plays a detrimental role in cardiac I/R injury using a rabbit myocardial I/R model. In this study, we sought to explore the specific mechanism of this autophagy-mediated cell damage in an in vitro simulated ischemia/reperfusion (sI/R) model using human umbilical vein endothelial cells. Our current study demonstrates that simulated I/R induces autophagy in a p65-Beclin 1-dependent manner, which can be suppressed with the inhibition of NF-κB. Furthermore, rapamycin which promotes autophagy, exacerbates sI/R-induced cell death. While 3-methyladenine rescues cell damage. Our data thus suggest that I/R promotes NF-κB p65 activity mediated Beclin 1-mediated autophagic flux, thereby exacerbating myocardial injury.
authors.affiliation: Medical Center, Hainan General Hospital, Haikou, 570311, China.
authors.forename: Min
authors.initials: M
authors.lastname: Zeng
authors.affiliation: Medical Center, Hainan General Hospital, Haikou, 570311, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Wei
authors.affiliation: Medical Center, Hainan General Hospital, Haikou, 570311, China.
authors.forename: Zhiyong
authors.initials: Z
authors.lastname: Wu
authors.affiliation: Medical Center, Hainan General Hospital, Haikou, 570311, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Li
authors.affiliation: Medical Center, Hainan General Hospital, Haikou, 570311, China.
authors.forename: Yin
authors.initials: Y
authors.lastname: Zheng
authors.affiliation: Medical Center, Hainan General Hospital, Haikou, 570311, China.
authors.forename: Bing
authors.initials: B
authors.lastname: Li
authors.affiliation: Medical Center, Hainan General Hospital, Haikou, 570311, China.
authors.forename: Xuqing
authors.initials: X
authors.lastname: Meng
authors.affiliation: Medical Center, Hainan General Hospital, Haikou, 570311, China.
authors.forename: Xiuhong
authors.initials: X
authors.lastname: Fu
authors.affiliation: Medical Center, Hainan General Hospital, Haikou, 570311, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Fei
mesh_terms: D000225:Adenine / Q000008:administration & dosage / Q000031:analogs & derivatives; D001343:Autophagy / Q000235:genetics*; D000071186:Beclin-1 / Q000235:genetics*; D016923:Cell Death / Q000187:drug effects / Q000235:genetics; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D015428:Myocardial Reperfusion Injury / Q000188:drug therapy / Q000235:genetics* / Q000473:pathology; D020123:Sirolimus / Q000008:administration & dosage; D051996:Transcription Factor RelA / Q000235:genetics*
chemical_list: C491997:BECN1 protein, human; D000071186:Beclin-1; D051996:Transcription Factor RelA; C025946:3-methyladenine; D000225:Adenine; D020123:Sirolimus