title: Polymorphism of SLC6A2 gene does not influence outcome of myocardial123I-mIBG scintigraphy in patients with chronic heart failure.
abstract: The NET, encoded by SLC6A2, is responsible for presynaptic NE-reuptake.123I-mIBG is clinically used to evaluate cardiac sympathetic function. However, it is unknown if polymorphism of SLC6A2 influences cardiac sympathetic activity as assessed with123I-mIBG. Therefore we studied the influence of SLC6A2 SNPs on myocardial123I-mIBG parameters in CHF.
  
  
  
  Forty-nine adults with stable CHF (age 66.5 ± 8.1 years, LVEF 22.3 ± 6.4) were enrolled. Fifteen minutes (early) and 4 hours (late) after administration of123I-mIBG planar images were acquired. The H/M ratio was calculated from the manually drawn ROI over the left ventricle and a fixed mediastinal ROI. Fourteen exons of the SLC6A2 gene were analyzed from whole blood samples.
  
  
  
  We found 6 different SLC6A2 SNPs, although none were functional. LVEF was the only independent predictor for early (adjusted R2= 0.063, p = 0.045) and late H/M ratio (adjusted R2= 0.116, p = 0.010). NT-proBNP was the only independent predictor for123I-mIBG WO (adjusted R2= 0.074, p = 0.032). SLC6A2 SNPs were not associated with any myocardial123I-mIBG-derived parameter.
  
  
  
  In this specific CHF population polymorphism of SLC6A2 gene was not associated with any123I-mIBG derived parameters.
authors.affiliation: Department of Nuclear Medicine, Academic Medical Center, University of Amsterdam, P.O. Box 22700, 1100 DE, Amsterdam, The Netherlands. d.o.verschure@amc.uva.nl.|Department of Cardiology, Zaans Medical Center, Zaandam, The Netherlands. d.o.verschure@amc.uva.nl.
authors.forename: Derk O
authors.initials: DO
authors.lastname: Verschure
authors.affiliation: Department of Genome Analysis, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands.
authors.forename: F
authors.initials: F
authors.lastname: Baas
authors.affiliation: Department of Nuclear Medicine, Academic Medical Center, University of Amsterdam, P.O. Box 22700, 1100 DE, Amsterdam, The Netherlands.
authors.forename: Berthe L F
authors.initials: BLF
authors.lastname: van Eck-Smit
authors.affiliation: Cardiology Centers of the Netherlands, Amsterdam, The Netherlands.
authors.forename: G Aernout
authors.initials: GA
authors.lastname: Somsen
authors.affiliation: Department of Nuclear Medicine, Academic Medical Center, University of Amsterdam, P.O. Box 22700, 1100 DE, Amsterdam, The Netherlands.
authors.forename: Hein J
authors.initials: HJ
authors.lastname: Verberne
mesh_terms: D019797:3-Iodobenzylguanidine; D000368:Aged; D002908:Chronic Disease; D005260:Female; D006333:Heart Failure / Q000000981:diagnostic imaging* / Q000235:genetics*; D006801:Humans; D007457:Iodine Radioisotopes; D008297:Male; D008875:Middle Aged; D050484:Norepinephrine Plasma Membrane Transport Proteins / Q000235:genetics*; D011110:Polymorphism, Genetic / Q000235:genetics*; D011877:Radionuclide Imaging; D019275:Radiopharmaceuticals; D013318:Stroke Volume
chemical_list: D007457:Iodine Radioisotopes; D050484:Norepinephrine Plasma Membrane Transport Proteins; D019275:Radiopharmaceuticals; C493227:SLC6A2 protein, human; D019797:3-Iodobenzylguanidine; C000614958:Iodine-123
keywords: Cardiac sympathetic activity; SLC6A2 gene; norepinephrine transporter; planar 123I-mIBG myocardial scintigraphy; polymorphism