title: Grafting with Arabidopsis thaliana.
abstract: Generating chimeric organisms is an invaluable way to study cell-to-cell movement and non-cell-autonomous actions of molecules. Plant grafting is an ancient method of generating chimeric organisms and recently has been used to study the movement of hormones, proteins, and RNAs. Here, I describe a simple and efficient way to graft Arabidopsis thaliana at the seedling stage to generate plants with roots and shoots of different genotypes. Using this protocol, success rates of over 80 % with up to 80 grafts assembled per hour can be achieved.
authors.affiliation: The Sainsbury Laboratory, University of Cambridge, Bateman Street, Cambridge, CB2 1LR, UK. cwm26@cam.ac.uk.
authors.forename: Charles W
authors.initials: CW
authors.lastname: Melnyk
mesh_terms: D017360:Arabidopsis / Q000235:genetics*; D029681:Arabidopsis Proteins / Q000235:genetics; D005838:Genotype; D010937:Plant Growth Regulators / Q000235:genetics; D018517:Plant Roots / Q000235:genetics; D018520:Plant Shoots / Q000235:genetics; D030821:Plants, Genetically Modified / Q000235:genetics*; D018749:RNA, Plant / Q000235:genetics; D036226:Seedlings / Q000235:genetics
chemical_list: D029681:Arabidopsis Proteins; D010937:Plant Growth Regulators; D018749:RNA, Plant
keywords: Arabidopsis thaliana; Chimeric plants; Graft-transmissible signal; Micro-grafting; Mobile molecules