title: Prevalence ofCampylobacter jejuniandCampylobacter coliin raw milk and some dairy products.
abstract: OBJECTIVE
  This study was accomplished to test raw milk and certain dairy products sold in local markets of Qena, Egypt, for the presence ofCampylobacter coliandCampylobacter jejuni.
  
  
  METHODS
  A total of 150 samples of raw milk, kareish cheese, and yoghurt (50 samples each) were subjected first to enrichment in Bolton broth at 42°C for 2 days under a microaerobic condition, subsequently campylobacter blood free selective agar plates were cultured and incubated in the same condition of the broth. Based on the morphological and biochemical themes of the growing colonies, it was further classified intoCampylobacterspp. The identified isolates were later affirmed by polymerase chain reaction using primers that were designed to locatehipO genes inC. jejuniandglyA inC. coli.
  
  
  RESULTS
  Of the total 150 examined samples of raw milk and soft cheese samples; 37 (24.6%) samples were contaminated withCampylobacterspp.C. jejuniwas dominating in this study in 20%, 14%, and 8% of the examined raw milk, kareish cheese, and yoghurt samples, respectively. No sample harboredC. coli.
  
  
  CONCLUSIONS
  Campylobacterspp. could be detected in 24.6% of the investigated samples.C. jejuniisolated from 14% of the total tested samples, whileC. colicould not be detected from the examined samples.Campylobacterspp. is rampant in the areas of poor hygienic conditions making products made from raw milk of public health hazard.
authors.affiliation: Department of Food Hygiene and Control, Faculty of Veterinary Medicine, South Valley University, Qena 83523, Egypt.
authors.forename: Mona A
authors.initials: MA
authors.lastname: El-Zamkan
authors.affiliation: Department of Food Hygiene and Control, Faculty of Veterinary Medicine, South Valley University, Qena 83523, Egypt.
authors.forename: Karima G Abdel
authors.initials: KG
authors.lastname: Hameed
keywords: Campylobacter coli; Campylobacter jejuni; dairy products; multiplex polymerase chain reaction; raw milk