title: Short communication: Labeling Listeria with anaerobic fluorescent protein for food safety studies.
abstract: Many food safety-related studies require the tracking of inoculated food-borne pathogens to monitor their fate in food complex environments. In the current study, we demonstrate the potential of plasmids containing the fluorescence protein gene evoglow-Pp1 (Evocatal, Dusseldorf, Germany) as a real-time reporter system for Listeria strains. This anaerobic fluorescent protein provides an easily detectable phenotype of microorganisms for food safety studies. This work is the first to report a reliable method to identify fluorescently labeled Listeria strains in food ecosystems.
authors.affiliation: Departamento de Tecnología de Alimentos, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), Carretera de la Coruña Km 7.5, 28040 Madrid, Spain. Electronic address: landete.josem@inia.es.
authors.forename: José M
authors.initials: JM
authors.lastname: Landete
authors.affiliation: Departamento de Tecnología de Alimentos, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), Carretera de la Coruña Km 7.5, 28040 Madrid, Spain.
authors.forename: Ángela
authors.initials: Á
authors.lastname: Peirotén
authors.affiliation: Departamento de Tecnología de Alimentos, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), Carretera de la Coruña Km 7.5, 28040 Madrid, Spain.
authors.forename: Margarita
authors.initials: M
authors.lastname: Medina
authors.affiliation: Departamento de Tecnología de Alimentos, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), Carretera de la Coruña Km 7.5, 28040 Madrid, Spain.
authors.forename: Juan L
authors.initials: JL
authors.lastname: Arqués
mesh_terms: D005516:Food Microbiology / Q000379:methods*; D008087:Listeria / Q000235:genetics*; D008089:Listeria monocytogenes / Q000235:genetics* / Q000302:isolation & purification; D008164:Luminescent Proteins / Q000032:analysis*; D010957:Plasmids / Q000235:genetics
chemical_list: D008164:Luminescent Proteins
keywords: Listeria; anaerobic fluorescent protein; food safety studies