title: PCR arrays indicate that the expression of extracellular matrix and cell adhesion genes in human adipocytes is regulated by IL-1β (interleukin-1β).
abstract: The role of IL-1β in regulating the expression of extracellular matrix (ECM) and cell adhesion genes in human adipocytes has been examined. Adipocytes differentiated in culture were incubated with IL-1β for 4 or 24 h and RNA probed with PCR arrays for 84 ECM and cell adhesion genes. Treatment with IL-1β resulted in changes in the expression at one or both time points of ∼50% of the genes probed by the arrays, the majority being down-regulated. Genes whose expression was down-regulated by IL-1β included those encoding several collagen chains and integrin subunits. In contrast, IL-1β induced substantial increases (>10-fold) in the expression of ICAM1, VCAM1, MMP1 and MMP3; the secretion of the encoded proteins was also markedly stimulated. IL-1β has a pervasive effect on the expression of ECM and cell adhesion genes in human adipocytes, consistent with the derangement of tissue structure during inflammation in white fat.
authors.affiliation: a Clore Laboratory, University of Buckingham , Buckingham , United Kingdom.
authors.forename: Malgorzata A
authors.initials: MA
authors.lastname: Kępczyńska
authors.affiliation: a Clore Laboratory, University of Buckingham , Buckingham , United Kingdom.
authors.forename: Mohamed S
authors.initials: MS
authors.lastname: Zaibi
authors.affiliation: b Zoology Department, College of Science, King Saud University , Riyadh , Saudi Arabia , and.
authors.forename: Suliman Y
authors.initials: SY
authors.lastname: Alomar
authors.affiliation: a Clore Laboratory, University of Buckingham , Buckingham , United Kingdom.|b Zoology Department, College of Science, King Saud University , Riyadh , Saudi Arabia , and.|c Obesity Biology Unit, University of Liverpool , Liverpool , United Kingdom.
authors.forename: Paul
authors.initials: P
authors.lastname: Trayhurn
mesh_terms: D052438:Adipocytes, White / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D015815:Cell Adhesion Molecules / Q000235:genetics / Q000378:metabolism*; D002454:Cell Differentiation; D002478:Cells, Cultured; D016326:Extracellular Matrix Proteins / Q000235:genetics / Q000378:metabolism*; D005260:Female; D005786:Gene Expression Regulation; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D053583:Interleukin-1beta / Q000235:genetics / Q000378:metabolism*; D020781:Matrix Metalloproteinase 1 / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D019278:Matrix Metalloproteinase 3 / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D008875:Middle Aged; D009765:Obesity / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D020411:Oligonucleotide Array Sequence Analysis; D060888:Real-Time Polymerase Chain Reaction; D011994:Recombinant Proteins / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D050151:Subcutaneous Fat / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D015854:Up-Regulation; D019010:Vascular Cell Adhesion Molecule-1 / Q000737:chemistry / Q000235:genetics / Q000378:metabolism
chemical_list: D015815:Cell Adhesion Molecules; D016326:Extracellular Matrix Proteins; C573420:ICAM1 protein, human; C508585:IL1B protein, human; D053583:Interleukin-1beta; D011994:Recombinant Proteins; D019010:Vascular Cell Adhesion Molecule-1; D018799:Intercellular Adhesion Molecule-1; C511768:MMP3 protein, human; D019278:Matrix Metalloproteinase 3; C522360:MMP1 protein, human; D020781:Matrix Metalloproteinase 1
keywords: Adipose tissue; ICAM1; MMPs; VCAM1; fibrosis