title: Identification of the Targets of Type III Secretion System Inhibitors.
abstract: A type III secretion system (T3SS) Inhibitor can be utilized for study in the research lab but also progressed into drug development. Since many pathogenic Gram-negative bacteria utilize this highly conserved system as a virulence factor, the prospect of the T3SS as a drug target is promising. To effectively move a T3SS inhibitor into the route of either research or pharmaceuticals an understanding of the target and mechanism of the inhibitor is required. Several methods can be utilized to identify the target. Included here is the use of knockout mutations, tagged inhibitor pull-down assays, and targeted identification methods.
authors.affiliation: Department of Biomedical Sciences, School of Medicine and Health Sciences, University of North Dakota, Grand Forks, ND, USA. Danielle.condry@med.und.edu.
authors.forename: Danielle L Jessen
authors.initials: DL
authors.lastname: Condry
authors.affiliation: Department of Biomedical Sciences, School of Medicine and Health Sciences, University of North Dakota, Grand Forks, ND, USA.
authors.forename: Matthew L
authors.initials: ML
authors.lastname: Nilles
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000037:antagonists & inhibitors* / Q000235:genetics / Q000378:metabolism; D015153:Blotting, Western; D055808:Drug Discovery; D004591:Electrophoresis, Polyacrylamide Gel; D055786:Gene Knockout Techniques; D006090:Gram-Negative Bacteria / Q000187:drug effects / Q000378:metabolism; D006461:Hemolysis / Q000187:drug effects; D009154:Mutation; D000069296:Type III Secretion Systems / Q000037:antagonists & inhibitors* / Q000235:genetics / Q000378:metabolism
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D000069296:Type III Secretion Systems
keywords: Knockout mutations; Pull-down assays; Secretion system inhibitor; Targeted inhibitor identification