title: Evaluation of DNA/Protein interactions and cytotoxic studies of copper(II) complexes incorporated with N, N donor ligands and terpyridine ligand.
abstract: A series of four new copper(II) heteroleptic complexes, [Cu(2‴-pytpy) (L)] (NO3)2·2H2O (1-4), where 2‴-pytpy=4'-(2'''-Pyridyl)-2, 2':6', 2''-terpyridine, L=bipyridyl (bpy), 1, 10 phenanthroline(phen), dipyridoquinoxaline(dpq) and dipyridophenazine (dppz) were synthesized and characterized by spectroscopic techniques. Further, the molecular structure of the complex (2) was confirmed by single crystal X-ray diffraction technique and the data revealed a penta coordinated, distorted square-pyramidal geometry with triclinic system. The interactions of four complexes with calf thymus DNA and bovine serum albumin (BSA) were investigated by electronic absorption, fluorescence and circular dichroism spectroscopy techniques. Spectral studies substantiated an intercalative binding mode of metal complexes with ct-DNA. Significant binding interactions of the complexes with protein have been further revealed from fluorescence studies. Furthermore, all the four complexes show potential cytotoxicity towards the human liver carcinoma cell line (HepG-2).
authors.affiliation: Department of Chemistry, School of Advanced Sciences, VIT University, Vellore, Tamilnadu 632014, India.
authors.forename: Kiran
authors.initials: K
authors.lastname: Tummalapalli
authors.affiliation: Bioinformatics Division, School of Bio Sciences and Technology, VIT University, Vellore, Tamil Nadu 632 014, India.
authors.forename: Vasavi
authors.initials: V
authors.lastname: C S
authors.affiliation: Center for Computational Natural Sciences and Bioinformatics, International Institute of Information Technology, Hyderabad 500 032, India.
authors.forename: Punnagai
authors.initials: P
authors.lastname: Munusami
authors.affiliation: Department of Chemistry, School of Advanced Sciences, VIT University, Vellore, Tamilnadu 632014, India. Electronic address: madhveshpathak@vit.ac.
authors.forename: Madhvesh
authors.initials: M
authors.lastname: Pathak
authors.affiliation: Department of Chemistry, School of Advanced Sciences, VIT University, Vellore, Tamilnadu 632014, India. Electronic address: balamurali.mm@vit.ac.in.
authors.forename: Balamurali
authors.initials: B
authors.lastname: M M
mesh_terms: D015082:2,2'-Dipyridyl / Q000737:chemistry; D000818:Animals; D000970:Antineoplastic Agents / Q000138:chemical synthesis* / Q000494:pharmacology; D001665:Binding Sites; D002413:Cations, Divalent; D002417:Cattle; D002470:Cell Survival / Q000187:drug effects; D056831:Coordination Complexes / Q000138:chemical synthesis* / Q000494:pharmacology; D003300:Copper / Q000737:chemistry; D004247:DNA / Q000737:chemistry; D056945:Hep G2 Cells; D006801:Humans; D007364:Intercalating Agents / Q000138:chemical synthesis* / Q000494:pharmacology; D007700:Kinetics; D008024:Ligands; D008968:Molecular Conformation; D062105:Molecular Docking Simulation; D009942:Organometallic Compounds / Q000138:chemical synthesis* / Q000494:pharmacology; D010618:Phenanthrolines / Q000737:chemistry; D010619:Phenazines / Q000737:chemistry; D011725:Pyridines / Q000737:chemistry; D011810:Quinoxalines / Q000138:chemical synthesis* / Q000494:pharmacology; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D013816:Thermodynamics
chemical_list: C517923:(2,2'-6',2'')-terpyridine; D000970:Antineoplastic Agents; D002413:Cations, Divalent; D056831:Coordination Complexes; D007364:Intercalating Agents; D008024:Ligands; D009942:Organometallic Compounds; D010618:Phenanthrolines; D010619:Phenazines; D011725:Pyridines; D011810:Quinoxalines; C460959:dipyridoquinoxaline copper(II) complex; D012710:Serum Albumin, Bovine; D015082:2,2'-Dipyridyl; D003300:Copper; D004247:DNA; C427203:calf thymus DNA; C025205:1,10-phenanthroline
keywords: Copper(II) complexes; DNA and protein binding studies; Density Functional Theory (DFT) calculations and cytotoxicity studies; Molecular docking