title: Analysis of the Complete Mitochondrial Genome Sequence of the Diploid CottonGossypium raimondiiby Comparative Genomics Approaches.
abstract: Cotton is one of the most important economic crops and the primary source of natural fiber and is an important protein source for animal feed. The complete nuclear and chloroplast (cp) genome sequences ofG. raimondiiare already available but not mitochondria. Here, we assembled the complete mitochondrial (mt) DNA sequence ofG. raimondiiinto a circular genome of length of 676,078 bp and performed comparative analyses with other higher plants. The genome contains 39 protein-coding genes, 6 rRNA genes, and 25 tRNA genes. We also identified four larger repeats (63.9 kb, 10.6 kb, 9.1 kb, and 2.5 kb) in this mt genome, which may be active in intramolecular recombination in the evolution of cotton. Strikingly, nearly all of theG. raimondiimt genome has been transferred to nucleus on Chr1, and the transfer event must be very recent. Phylogenetic analysis reveals thatG. raimondii, as a member of Malvaceae, is much closer to another cotton (G. barbadense) than other rosids, and the clade formed by twoGossypiumspecies is sister to Brassicales. TheG. raimondiimt genome may provide a crucial foundation for evolutionary analysis, molecular biology, and cytoplasmic male sterility in cotton and other higher plants.
authors.affiliation: College of Information Science and Technology, Nanjing Forestry University, Nanjing, Jiangsu, China.
authors.forename: Changwei
authors.identifier: 0000-0002-6951-2464
authors.initials: C
authors.lastname: Bi
authors.affiliation: Plant Genome Mapping Laboratory, University of Georgia, Athens, GA 30602, USA.
authors.forename: Andrew H
authors.initials: AH
authors.lastname: Paterson
authors.affiliation: College of Information Science and Technology, Nanjing Forestry University, Nanjing, Jiangsu, China.
authors.forename: Xuelin
authors.initials: X
authors.lastname: Wang
authors.affiliation: College of Information Science and Technology, Nanjing Forestry University, Nanjing, Jiangsu, China.
authors.forename: Yiqing
authors.initials: Y
authors.lastname: Xu
authors.affiliation: College of Information Science and Technology, Nanjing Forestry University, Nanjing, Jiangsu, China; The Southern Modern Forestry Collaborative Innovation Center, Nanjing Forestry University, Nanjing, Jiangsu, China.
authors.forename: Dongyang
authors.initials: D
authors.lastname: Wu
authors.affiliation: College of Information Science and Technology, Nanjing Forestry University, Nanjing, Jiangsu, China.
authors.forename: Yanshu
authors.initials: Y
authors.lastname: Qu
authors.affiliation: College of Information Science and Technology, Nanjing Forestry University, Nanjing, Jiangsu, China.
authors.forename: Anna
authors.initials: A
authors.lastname: Jiang
authors.affiliation: College of Information Science and Technology, Nanjing Forestry University, Nanjing, Jiangsu, China.
authors.forename: Qiaolin
authors.initials: Q
authors.lastname: Ye
authors.affiliation: College of Information Science and Technology, Nanjing Forestry University, Nanjing, Jiangsu, China; The Southern Modern Forestry Collaborative Innovation Center, Nanjing Forestry University, Nanjing, Jiangsu, China.
authors.forename: Ning
authors.identifier: 0000-0001-7249-8352
authors.initials: N
authors.lastname: Ye
mesh_terms: D001483:Base Sequence; D002467:Cell Nucleus / Q000235:genetics; D004270:DNA, Circular / Q000235:genetics; D018744:DNA, Plant / Q000235:genetics; D004171:Diploidy; D017343:Genes, Plant; D014644:Genetic Variation; D054628:Genome, Chloroplast / Q000235:genetics; D054629:Genome, Mitochondrial; D018745:Genome, Plant; D023281:Genomics / Q000379:methods*; D003368:Gossypium / Q000235:genetics*; D007438:Introns / Q000235:genetics; D016013:Likelihood Functions; D018895:Microsatellite Repeats / Q000235:genetics; D005810:Multigene Family; D016366:Open Reading Frames / Q000235:genetics; D010802:Phylogeny; D017393:RNA Editing / Q000235:genetics; D012343:RNA, Transfer / Q000235:genetics; D020040:Trans-Splicing / Q000235:genetics
chemical_list: D004270:DNA, Circular; D018744:DNA, Plant; D012343:RNA, Transfer