title: Isolation and molecular characterization of Clostridium perfringens from healthy Merino lambs in Patagonia region, Argentina.
abstract: The presence and molecular characterization of Clostridium perfringens in healthy Merino lambs over a six-month period was investigated in this study. Overall, a high prevalence of C. perfringens was detected, even in day-old lambs. Even though the majority of the isolates were characterized as being of type A, types C and D were also isolated. Furthermore, a high genetic diversity was observed by PFGE among the type A isolates.
authors.affiliation: National Institute for Agricultural Technology, Bariloche Research Station, CONICET, Bote Modesta Victoria 4450, San Carlos de Bariloche, R8403DVZ, Río Negro, Argentina. Electronic address: mignaqui.anaclara@inta.gob.ar.
authors.forename: A C
authors.initials: AC
authors.lastname: Mignaqui
authors.affiliation: National Institute for Agricultural Technology, Bariloche Research Station, Bote Modesta Victoria 4450, San Carlos de Bariloche, R8403DVZ, Río Negro, Argentina.
authors.forename: R B
authors.initials: RB
authors.lastname: Marcellino
authors.affiliation: Technical University of Denmark, National Veterinary Institute, Bülowsvej 27, 1870, Frederiksberg, Denmark.
authors.forename: T
authors.initials: T
authors.lastname: Ronco
authors.affiliation: National Institute for Agricultural Technology, Bariloche Research Station, Bote Modesta Victoria 4450, San Carlos de Bariloche, R8403DVZ, Río Negro, Argentina.
authors.forename: J S
authors.initials: JS
authors.lastname: Pappalardo
authors.affiliation: Technical University of Denmark, National Veterinary Institute, Bülowsvej 27, 1870, Frederiksberg, Denmark.
authors.forename: B
authors.initials: B
authors.lastname: Nonnemann
authors.affiliation: Technical University of Denmark, National Veterinary Institute, Bülowsvej 27, 1870, Frederiksberg, Denmark.
authors.forename: K
authors.initials: K
authors.lastname: Pedersen
authors.affiliation: National Institute for Agricultural Technology, Bariloche Research Station, Bote Modesta Victoria 4450, San Carlos de Bariloche, R8403DVZ, Río Negro, Argentina.
authors.forename: C A
authors.initials: CA
authors.lastname: Robles
mesh_terms: D000818:Animals; D001118:Argentina / Q000453:epidemiology; D003015:Clostridium Infections / Q000453:epidemiology / Q000382:microbiology / Q000662:veterinary*; D003016:Clostridium perfringens / Q000235:genetics* / Q000302:isolation & purification; D016521:Electrophoresis, Gel, Pulsed-Field / Q000662:veterinary; D005260:Female; D005767:Gastrointestinal Diseases / Q000453:epidemiology / Q000382:microbiology / Q000662:veterinary*; D014644:Genetic Variation; D016133:Polymerase Chain Reaction / Q000662:veterinary; D015995:Prevalence; D012756:Sheep; D012757:Sheep Diseases / Q000453:epidemiology / Q000382:microbiology*
keywords: Clostridium perfringens; Enterotoxaemia; Lambs; PCR; PFGE