title: Genetic diversity of bovine viral diarrhea viruses from the Galicia region of Spain.
abstract: This study examined the frequency and diversity of bovine viral diarrhoea viruses (BVDVs) infecting cattle in Galicia (northwestern Spain). A total of 86 BVDV strains were typed in samples of serum from 79 persistently infected animals and 3 viraemic animals and of abomasal fluid from 4 fetuses. Samples came from 73 farms participating in a voluntary BVDV control programme. Typing was based on a 288-bp sequence from the 5' untranslated region amplified using primers 324 and 326. Of the 86 strains, 85 (98.8 per cent) belonged to species BVDV-1 and 1 (1.2 per cent) belonged to BVDV-2; 73 strains (84.9 per cent) were typed as BVDV-1b, 2 as BVDV-1e and 6 as BVDV-1d. One strain each was typed as belonging to 1a, 1h, 1k and 1l. The sole BVDV-2 strain was classified as 2a. These results identify BVDV-1b as the predominant species, and they indicate the presence of viral types not previously described anywhere in Spain. This is also the first report of BVDV-2 in Galicia and only the second report of BVDV-2 in Spain.
authors.affiliation: Lalín , Spain.
authors.forename: C
authors.initials: C
authors.lastname: Factor
authors.affiliation: Veterinary Faculty of Lugo , Institute of Food Analysis and Research (Animal Health and Epidemiology Unit), Santiago de Compostela University , Lugo , Spain.
authors.forename: E
authors.initials: E
authors.lastname: Yus
authors.affiliation: Animal Health and Production Laboratory of Galicia , Lugo , Spain.
authors.forename: C
authors.initials: C
authors.lastname: Eiras
authors.affiliation: Veterinary Faculty of Lugo , Institute of Food Analysis and Research (Animal Health and Epidemiology Unit), Santiago de Compostela University , Lugo , Spain.
authors.forename: M L
authors.initials: ML
authors.lastname: Sanjuan
authors.affiliation: Boehringer Ingelheim España , Sant Cugat del Vallès , Barcelona.
authors.forename: M
authors.initials: M
authors.lastname: Cerviño
authors.affiliation: Center of Agrarian Research , Galicia , Spain.
authors.forename: I
authors.initials: I
authors.lastname: Arnaiz
authors.affiliation: Anatomy and Animal Production Department, Veterinary Faculty of Lugo , Santiago de Compostela University , Lugo , Spain.
authors.forename: F J
authors.initials: FJ
authors.lastname: Diéguez
keywords: Bovine viral diarrhea virus; Cattle; PCR; Pestivirus; Phylogenetic analysis