title: Characterization of BcMF23a and BcMF23b, two putative pectin methylesterase genes related to pollen development in Brassica campestris ssp. chinensis.
abstract: Two homologous genes, Brassica campestris Male Fertility 23a (BcMF23a) and Brassica campestris Male Fertility 23b (BcMF23b), encoding putative pectin methylesterases (PMEs) were isolated from Brassica campestris ssp. chinensis (syn. Brassica rapa ssp. chinensis). These two genes sharing high sequence identity with each other were highly expressed in the fertile flower buds but silenced in the sterile ones of genic male sterile line system ('Bcajh97-01A/B'). Results of RT-PCR and in situ hybridization suggested that BcMF23a and BcMF23b were pollen-expressed genes, whose transcripts were first detected at the binucleate pollen and maintained throughout to the mature pollen grains. Western blot indicated that both of the putative BcMF23a and BcMF23b proteins are approximately 40 kDa, which exhibited extracellular localization revealed by transient expression analysis in the onion epidermal cells. The promoter of BcMF23a was active specifically in pollen during the late pollen developmental stages, while, in addition to the pollen, BcMF23b promoter drove an extra gene expression in the valve margins, abscission layer at the base of the first true leaves, taproot and lateral roots in seedlings.
authors.affiliation: Laboratory of Cell and Molecular Biology, Institute of Vegetable Science, Zhejiang University, Hangzhou, 310058, China.|Key Laboratory of Horticultural Plant Growth, Development and Quality Improvement, Ministry of Agriculture, Hangzhou, 310058, China.
authors.forename: Sue
authors.initials: S
authors.lastname: Lin
authors.affiliation: Laboratory of Cell and Molecular Biology, Institute of Vegetable Science, Zhejiang University, Hangzhou, 310058, China.|Key Laboratory of Horticultural Plant Growth, Development and Quality Improvement, Ministry of Agriculture, Hangzhou, 310058, China.
authors.forename: Li
authors.initials: L
authors.lastname: Huang
authors.affiliation: Laboratory of Cell and Molecular Biology, Institute of Vegetable Science, Zhejiang University, Hangzhou, 310058, China.|Key Laboratory of Horticultural Plant Growth, Development and Quality Improvement, Ministry of Agriculture, Hangzhou, 310058, China.
authors.forename: Xiaolin
authors.initials: X
authors.lastname: Yu
authors.affiliation: Laboratory of Cell and Molecular Biology, Institute of Vegetable Science, Zhejiang University, Hangzhou, 310058, China.|Key Laboratory of Horticultural Plant Growth, Development and Quality Improvement, Ministry of Agriculture, Hangzhou, 310058, China.
authors.forename: Xingpeng
authors.initials: X
authors.lastname: Xiong
authors.affiliation: Laboratory of Cell and Molecular Biology, Institute of Vegetable Science, Zhejiang University, Hangzhou, 310058, China.|Key Laboratory of Horticultural Plant Growth, Development and Quality Improvement, Ministry of Agriculture, Hangzhou, 310058, China.
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Yue
authors.affiliation: Laboratory of Cell and Molecular Biology, Institute of Vegetable Science, Zhejiang University, Hangzhou, 310058, China.|Key Laboratory of Horticultural Plant Growth, Development and Quality Improvement, Ministry of Agriculture, Hangzhou, 310058, China.
authors.forename: Tingting
authors.initials: T
authors.lastname: Liu
authors.affiliation: Laboratory of Cell and Molecular Biology, Institute of Vegetable Science, Zhejiang University, Hangzhou, 310058, China.|Key Laboratory of Horticultural Plant Growth, Development and Quality Improvement, Ministry of Agriculture, Hangzhou, 310058, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Liang
authors.affiliation: Laboratory of Cell and Molecular Biology, Institute of Vegetable Science, Zhejiang University, Hangzhou, 310058, China.|Key Laboratory of Horticultural Plant Growth, Development and Quality Improvement, Ministry of Agriculture, Hangzhou, 310058, China.
authors.forename: Meiling
authors.initials: M
authors.lastname: Lv
authors.affiliation: Laboratory of Cell and Molecular Biology, Institute of Vegetable Science, Zhejiang University, Hangzhou, 310058, China. jshcao@zju.edu.cn.|Key Laboratory of Horticultural Plant Growth, Development and Quality Improvement, Ministry of Agriculture, Hangzhou, 310058, China. jshcao@zju.edu.cn.
authors.forename: Jiashu
authors.initials: J
authors.lastname: Cao
mesh_terms: D001937:Brassica / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development; D002265:Carboxylic Ester Hydrolases / Q000235:genetics* / Q000378:metabolism*; D003001:Cloning, Molecular / Q000379:methods*; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D018506:Gene Expression Regulation, Plant; D008970:Molecular Weight; D010368:Pectins / Q000378:metabolism; D051479:Plant Infertility; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D011058:Pollen / Q000235:genetics / Q000254:growth & development*; D011401:Promoter Regions, Genetic
chemical_list: D010940:Plant Proteins; D010368:Pectins; D002265:Carboxylic Ester Hydrolases; C022323:pectinesterase
keywords: Brassica campestris; Brassica rapa; Male fertility; Pectin methylesterase; Pollen