title: Pig BVDV-2 non-structural protein (Npro) links to cellular antiviral response in vitro.
abstract: In this study, we constructed for the first time a full-length cDNA clone of pig-original bovine viral diarrhea virus 2 (BVDV-2) strain SH-28, modified the cDNA clone (pASH28) for mutant pASHΔNproand derived virus strain vASHΔNproby deleting the genomic region encoding the Npropolypeptide, and examined significance of protein Nprofor antiviral responses in vitro. Data showed that Npro-deletion mutant virus vASHΔNproled to significant overexpression of oligo adenylate synthetase (OAS), myxovirus-resistant protein 1 (Mx1), and ubiquitin-like protein 15 (ISG15). Data also revealed that overexpression of Npro, but not NS2 and NS3 proteins, resulted in significant down-regulation of OAS, Mx1, and ISG15 production (p ≤ 0.05) in bovine cells as well as porcine cells transfected with Nprorecombinant eukaryotic expression plasmids. Npro(but not NS2 and NS3) was also found to inhibit poly(IC) from inducing production of type I interferon (IFN-I). These results indicated that protein Npromay play multiple roles in regulating antiviral response in host cells interfered by pig BVDV-2 strain, and provided useful information to understand better the mechanism of BVDV-2 persistent infection in pigs.
authors.affiliation: College of Veterinary Medicine, and Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou, 225009, China.|Institute of Animal Sciences and Veterinary Medicine, Shanghai Academy of Agricultural Sciences, Shanghai, 201106, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Tao
authors.affiliation: College of Veterinary Medicine, and Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou, 225009, China.
authors.forename: Jinhu
authors.initials: J
authors.lastname: Liao
authors.affiliation: College of Veterinary Medicine, and Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou, 225009, China.
authors.forename: Jianye
authors.initials: J
authors.lastname: Wang
authors.affiliation: College of Veterinary Medicine, and Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou, 225009, China.
authors.forename: Xinjun
authors.initials: X
authors.lastname: Zhang
authors.affiliation: College of Veterinary Medicine, and Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou, 225009, China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: College of Veterinary Medicine, and Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou, 225009, China.
authors.forename: Liqian
authors.initials: L
authors.lastname: Zhu
authors.affiliation: College of Veterinary Medicine, Kansas State University, Manhattan, KS, 66506, USA.
authors.forename: Weiping
authors.initials: W
authors.lastname: Zhang
authors.affiliation: Institute of Animal Sciences and Veterinary Medicine, Shanghai Academy of Agricultural Sciences, Shanghai, 201106, China.
authors.forename: Huili
authors.initials: H
authors.lastname: Liu
authors.affiliation: College of Veterinary Medicine, and Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou, 225009, China. yzgqzhu@yzu.edu.cn.
authors.forename: Guoqiang
authors.initials: G
authors.lastname: Zhu
mesh_terms: D000818:Animals; D002417:Cattle; D002460:Cell Line; D030242:Diarrhea Virus 2, Bovine Viral / Q000235:genetics* / Q000472:pathogenicity; D015967:Gene Expression Regulation, Viral; D064547:Myxovirus Resistance Proteins / Q000096:biosynthesis / Q000235:genetics*; D018182:Pestivirus Infections / Q000235:genetics* / Q000821:virology; D013552:Swine / Q000821:virology; D014452:Ubiquitins / Q000096:biosynthesis / Q000235:genetics; D017361:Viral Nonstructural Proteins / Q000096:biosynthesis / Q000235:genetics*
chemical_list: D064547:Myxovirus Resistance Proteins; D014452:Ubiquitins; D017361:Viral Nonstructural Proteins
keywords: Antiviral response; BVDV-2; Infectious cDNA clone; Non-structural protein; Pig