title: TP53 codon 72 polymorphism and breast cancer risk in Bangladeshi population.
abstract: BACKGROUND
  Breast cancer, a hereditary or heterogeneous sporadic disease, is the most common cancer in women worldwide. The tumor suppressor TP53 gene has been found to be the most commonly mutated genes in many types of human cancers, including breast cancer. This study aimed to investigate the association of codon 72 polymorphism of TP53 gene with breast cancer risk in Bangladeshi females.
  
  
  METHODS
  The study included 125 cases and 125 healthy controls. Genotyping and polymorphism were determined by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis.
  
  
  RESULTS
  The frequencies of the three genotypes Arg/Arg, Arg/Pro, and Pro/Pro were 43.2, 33.6, and 23.2% in cases, whereas 48.8, 40.8, and 10.4% in controls, respectively. The frequency of mutant homozygous (Pro/Pro) genotype was significantly increased in breast cancer patients as compared with controls (23.2 vs 10.4%), and showed 2.52-fold significantly increased risk for breast cancer (OR 2.5199, 95% CI 1.19-5.33, p = 0.0157). The frequencies of Pro/Pro genotype were significantly higher in breast cancer cases with non-breast feeding status. Pro allele frequency was found to be significantly increased in breast cancer cases (OR 1.4978, 95% CI 1.0357-2.1662, p = 0.0318).
  
  
  CONCLUSIONS
  Our data suggest that mutant (Pro/Pro) homozygosity at codon 72 of TP53 gene is significantly associated with breast cancer susceptibility in Bangladeshi women. In addition, this association was significantly related to lactating status.
authors.affiliation: Pharmacy Discipline, Life Science School, Khulna University, Khulna, 9208, Bangladesh.
authors.forename: Amir
authors.initials: A
authors.lastname: Hossain
authors.affiliation: Pharmacy Discipline, Life Science School, Khulna University, Khulna, 9208, Bangladesh.
authors.forename: Gazi Md Monjur
authors.initials: GMM
authors.lastname: Murshid
authors.affiliation: Pharmacy Discipline, Life Science School, Khulna University, Khulna, 9208, Bangladesh.
authors.forename: Md Nazmul Hasan
authors.initials: MNH
authors.lastname: Zilani
authors.affiliation: Pharmacy Discipline, Life Science School, Khulna University, Khulna, 9208, Bangladesh.|Department of Medicinal Chemistry, University of Zagreb, Zagreb, 10000, Croatia.
authors.forename: Fahrima
authors.initials: F
authors.lastname: Islam
authors.affiliation: MARKS Dental College, Dhaka, Bangladesh.
authors.forename: Razia
authors.initials: R
authors.lastname: Sultana
authors.affiliation: Soonchunhyang University, Asan, South Korea.
authors.forename: Tamanna
authors.initials: T
authors.lastname: Sultana
authors.affiliation: Pharmacy Discipline, Life Science School, Khulna University, Khulna, 9208, Bangladesh.
authors.forename: Md Golam
authors.initials: MG
authors.lastname: Hossain
authors.affiliation: Pharmacy Discipline, Life Science School, Khulna University, Khulna, 9208, Bangladesh. dipti0103@yahoo.com.
authors.forename: Md Mustafizur
authors.identifier: http://orcid.org/0000-0001-8585-263X
authors.initials: MM
authors.lastname: Rahman
mesh_terms: D000328:Adult; D000368:Aged; D001459:Bangladesh / Q000453:epidemiology; D014408:Biomarkers, Tumor / Q000235:genetics*; D001943:Breast Neoplasms / Q000453:epidemiology / Q000235:genetics* / Q000473:pathology; D016022:Case-Control Studies; D003062:Codon / Q000235:genetics*; D005260:Female; D005500:Follow-Up Studies; D020022:Genetic Predisposition to Disease; D006801:Humans; D008875:Middle Aged; D011110:Polymorphism, Genetic; D011379:Prognosis; D012307:Risk Factors; D016159:Tumor Suppressor Protein p53 / Q000235:genetics*
chemical_list: D014408:Biomarkers, Tumor; D003062:Codon; C495901:TP53 protein, human; D016159:Tumor Suppressor Protein p53
keywords: Bangladesh; Breast cancer; Codon 72 polymorphism; SNP; TP53