title: Development of SSR markers for a Tibetan medicinal plant,Lancea tibetica(Phrymaceae), based on RAD sequencing.
abstract: OBJECTIVE
  Lancea tibetica(Phrymaceae), a Tibetan medicinal plant, is endemic to the Qinghai-Tibet Plateau. The over-exploitation of wildL. tibeticahas led to the destruction of many populations. To enhance protection and management, biological research, especially population genetic studies, should be carried out onL. tibetica. Simple sequence repeat (SSR) markers ofL. tibeticawere developed to analyze population diversity.
  
  
  RESULTS
  Four thousand four hundred and forty-one SSR loci were identified forL. tibeticabased on restriction-site associated DNA (RAD) sequencing on the Illumina HiSeq platform. One hundred SSR loci were arbitrarily selected for primer design, and 38 of them were successfully amplified. These markers were tested on 56 individuals from three populations ofL. tibetica, and 10 markers displayed polymorphisms. The total number of alleles per locus ranged from three to eight, and observed and expected heterozygosities ranged from 0.200 to 1.000 and 0.683 to 0.879, respectively. We tested for cross-amplification of these 10 markers in the related speciesL. hirsutaand found that nine could be successfully amplified.
  
  
  CONCLUSIONS
  The SSR markers characterized here are the first to be developed and tested inL. tibetica. They will be useful for future population genetic studies onL. tibeticaand closely related species.
authors.affiliation: Key Laboratory of Adaptation and Evolution of Plateau Biota, Northwest Institute of Plateau Biology, Northwest Institute of Eco-Environment and Resources, Chinese Academy of Sciences, Xining 810001, People's Republic of China; University of Chinese Academy of Sciences, Beijing 100039, People's Republic of China.
authors.forename: Zunzhe
authors.initials: Z
authors.lastname: Tian
authors.affiliation: Key Laboratory of Adaptation and Evolution of Plateau Biota, Northwest Institute of Plateau Biology, Northwest Institute of Eco-Environment and Resources, Chinese Academy of Sciences, Xining 810001, People's Republic of China.
authors.forename: Faqi
authors.initials: F
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Adaptation and Evolution of Plateau Biota, Northwest Institute of Plateau Biology, Northwest Institute of Eco-Environment and Resources, Chinese Academy of Sciences, Xining 810001, People's Republic of China; University of Chinese Academy of Sciences, Beijing 100039, People's Republic of China.
authors.forename: Hairui
authors.initials: H
authors.lastname: Liu
authors.affiliation: Key Laboratory of Adaptation and Evolution of Plateau Biota, Northwest Institute of Plateau Biology, Northwest Institute of Eco-Environment and Resources, Chinese Academy of Sciences, Xining 810001, People's Republic of China.
authors.forename: Qingbo
authors.initials: Q
authors.lastname: Gao
authors.affiliation: Key Laboratory of Adaptation and Evolution of Plateau Biota, Northwest Institute of Plateau Biology, Northwest Institute of Eco-Environment and Resources, Chinese Academy of Sciences, Xining 810001, People's Republic of China.
authors.forename: Shilong
authors.initials: S
authors.lastname: Chen
keywords: Lancea tibetica; Phrymaceae; RAD sequencing; population diversity; simple sequence repeat (SSR)