title: Illumina Sequencing of Bisulfite-Converted DNA Libraries.
abstract: Here we describe a standard MethylC-seq protocol using single-read sequencing on an Illumina Genome Analyzer II platform. The protocol involves ligation of methylated sequencing adaptors to sonicated genomic DNA, gel purification, sodium bisulfite conversion, polymerase chain reaction (PCR) amplification, and sequencing.
authors.forename: Paul M
authors.initials: PM
authors.lastname: Lizardi
authors.forename: Qin
authors.initials: Q
authors.lastname: Yan
authors.forename: Narendra
authors.initials: N
authors.lastname: Wajapeyee
mesh_terms: D011088:DNA Ligases / Q000378:metabolism; D017931:DNA Primers / Q000378:metabolism; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA / Q000379:methods*; D013447:Sulfites / Q000378:metabolism
chemical_list: D017931:DNA Primers; D013447:Sulfites; D011088:DNA Ligases; C042345:hydrogen sulfite