title: Variation in pestivirus growth in testicle primary cell culture is more dependent on the individual cell donor than cattle breed.
abstract: The causes of bovine respiratory disease complex (BRDC) are multifactorial and include infection with both viral and bacterial pathogens. Host factors are also involved as different breeds of cattle appear to have different susceptibilities to BRDC. Infection with bovine pestiviruses, including bovine viral diarrhea virus 1 (BVDV1), BVDV2 and 'HoBi'-like viruses, is linked to the development of BRDC. The aim of the present study was to compare the growth of different bovine pestiviruses in primary testicle cell cultures obtained from taurine, indicine and mixed taurine and indicine cattle breeds. Primary cells strains, derived from testicular tissue, were generated from three animals from each breed. Bovine pestivirus strains used were from BVDV-1a, BVDV-1b, BVDV-2a and 'HoBi'-like virus. Growth was compared by determining virus titers after one passage in primary cells. All tests were run in triplicate. Virus titers were determined by endpoint dilution and RT-qPCR. Statistical analysis was performed using one way analysis of variance (ANOVA) followed by the Tukey's Multiple Comparison Test (P˂0.05). Significant differences in virus growth did not correlate with cattle breed. However, significant differences were observed between cells derived from different individuals regardless of breed. Variation in the replication of virus in primary cell strains may reflect a genetic predisposition that favors virus replication.
authors.affiliation: Laboratório de Virologia, Faculdade de Veterinária, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, Rio Grande do Sul, Brazil.
authors.forename: Matheus N
authors.initials: MN
authors.lastname: Weber
authors.affiliation: Ruminant Diseases and Immunology Research Unit, Agricultural Research Service (ARS), National Animal Disease Center (NADC), United States Department of Agriculture (USDA), 1920 Dayton Avenue, PO Box 70, Ames, IA, 50010, USA.
authors.forename: Fernando V
authors.initials: FV
authors.lastname: Bauermann
authors.affiliation: Laboratorio de Vacunología y Constatación, Facultad de Medicina Veterinaria y Zootecnia (FMVZ), Universidad Nacional Autónoma de México (UNAM), Ciudad de Mexico, Distrito Federal, Mexico.
authors.forename: Ninnet
authors.initials: N
authors.lastname: Gómez-Romero
authors.affiliation: Department of Animal Science, Texas A&M University (TAMU), College Station, TX, USA.
authors.forename: Andy D
authors.initials: AD
authors.lastname: Herring
authors.affiliation: Laboratório de Virologia, Faculdade de Veterinária, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, Rio Grande do Sul, Brazil.
authors.forename: Cláudio W
authors.initials: CW
authors.lastname: Canal
authors.affiliation: Ruminant Diseases and Immunology Research Unit, Agricultural Research Service (ARS), National Animal Disease Center (NADC), United States Department of Agriculture (USDA), 1920 Dayton Avenue, PO Box 70, Ames, IA, 50010, USA.
authors.forename: John D
authors.initials: JD
authors.lastname: Neill
authors.affiliation: Ruminant Diseases and Immunology Research Unit, Agricultural Research Service (ARS), National Animal Disease Center (NADC), United States Department of Agriculture (USDA), 1920 Dayton Avenue, PO Box 70, Ames, IA, 50010, USA. julia.ridpath@ars.usda.gov.
authors.forename: Julia F
authors.initials: JF
authors.lastname: Ridpath
mesh_terms: D000818:Animals; D001912:Bovine Virus Diarrhea-Mucosal Disease / Q000821:virology*; D002417:Cattle; D001908:Diarrhea Viruses, Bovine Viral / Q000254:growth & development / Q000502:physiology*; D054884:Host-Pathogen Interactions / Q000235:genetics; D008297:Male; D061251:Primary Cell Culture; D013737:Testis / Q000166:cytology / Q000821:virology*; D014779:Virus Replication
keywords: Bovine; Cell culture; Indicine; Pestivirus; Taurine