title: Live Monitoring of Blastemal Cell Contributions during Appendage Regeneration.
abstract: The blastema is a mass of progenitor cells that enables regeneration of amputated salamander limbs or fish fins. Methodology to label and track blastemal cell progeny has been deficient, restricting our understanding of appendage regeneration. Here, we created a system for clonal analysis and quantitative imaging of hundreds of blastemal cells and their respective progeny in living adult zebrafish undergoing fin regeneration. Amputation stimulates resident cells within a limited recruitment zone to reset proximodistal (PD) positional information and assemble the blastema. Within the newly formed blastema, the spatial coordinates of connective tissue progenitors are predictive of their ultimate contributions to regenerated skeletal structures, indicating early development of an approximate PD pre-pattern. Calcineurin regulates size recovery by controlling the average number of progeny divisions without disrupting this pre-pattern. Our longitudinal clonal analyses of regenerating zebrafish fins provide evidence that connective tissue progenitors are rapidly organized into a scalable blueprint of lost structures.
authors.affiliation: Department of Cell Biology, Duke University Medical Center, Durham, NC 27710, USA.
authors.forename: Valerie A
authors.initials: VA
authors.lastname: Tornini
authors.affiliation: Candiolo Cancer Institute FPO-IRCCS, Candiolo, Turin 10060, Italy.
authors.forename: Alberto
authors.initials: A
authors.lastname: Puliafito
authors.affiliation: Department of Cell Biology, Duke University Medical Center, Durham, NC 27710, USA.
authors.forename: Leslie A
authors.initials: LA
authors.lastname: Slota
authors.affiliation: Department of Cell Biology, Duke University Medical Center, Durham, NC 27710, USA.
authors.forename: John D
authors.initials: JD
authors.lastname: Thompson
authors.affiliation: Department of Cell Biology, Duke University Medical Center, Durham, NC 27710, USA.
authors.forename: Gregory
authors.initials: G
authors.lastname: Nachtrab
authors.affiliation: IBENS, INSERM U1024, CNRS UMR 8197, Paris 75005, France.
authors.forename: Anna-Lila
authors.initials: AL
authors.lastname: Kaushik
authors.affiliation: IBENS, INSERM U1024, CNRS UMR 8197, Paris 75005, France.
authors.forename: Marika
authors.initials: M
authors.lastname: Kapsimali
authors.affiliation: Candiolo Cancer Institute FPO-IRCCS, Candiolo, Turin 10060, Italy; Department of Oncology, University of Torino, Turin 10060, Italy.
authors.forename: Luca
authors.initials: L
authors.lastname: Primo
authors.affiliation: Department of Cell Biology, Duke University Medical Center, Durham, NC 27710, USA.
authors.forename: Stefano
authors.initials: S
authors.lastname: Di Talia
authors.affiliation: Department of Cell Biology, Duke University Medical Center, Durham, NC 27710, USA. Electronic address: kenneth.poss@duke.edu.
authors.forename: Kenneth D
authors.initials: KD
authors.lastname: Poss
mesh_terms: D058500:Animal Fins / Q000502:physiology*; D000818:Animals; D019703:Calcineurin / Q000378:metabolism; D005260:Female; D008297:Male; D012038:Regeneration; D013234:Stem Cells; D015027:Zebrafish / Q000502:physiology*; D029961:Zebrafish Proteins / Q000378:metabolism
chemical_list: D029961:Zebrafish Proteins; D019703:Calcineurin
keywords: appendage; blastema; clonal analysis; fibroblast; fin; positional identity; regeneration; tph1b; vertebrate; zebrafish