title: Assessment of chlorpyrifos toxic effects in zebrafish (Danio rerio) metabolism.
abstract: In this work the effect of chlorpyrifos exposure on metabolic profiles of zebrafish muscle was evaluated by liquid chromatography coupled to high resolution mass spectrometry. Different chemometric tools based on the selection of Regions of Interest and on Multivariate Curve-Resolution-Alternating Least Squares are proposed for the analysis of the complex data sets generated in the different exposure experiments. Analysis of Variance Simultaneous Component Analysis of changes on metabolite peak profile areas showed significant chlorpyrifos concentration and exposure time-dependent changes, clearly differentiating between exposed and non-exposed samples and between short (2 h) and long exposure times (6 h or 24 h). The changes observed in the concentrations of 50 muscle metabolites are indicative of induction of oxidative stress, of a general disruption of neurotransmitter metabolism, and of muscle exhaustion. These three effects are intimately related to the toxicity of chlorpyrifos.
authors.affiliation: Department of Environmental Chemistry, IDAEA-CSIC, Jordi Girona 18-26, 08034 Barcelona, Catalonia, Spain. Electronic address: cristian.gomez@cid.csic.es.
authors.forename: Cristian
authors.initials: C
authors.lastname: Gómez-Canela
authors.affiliation: Centre d'Investigació I Desenvolupament, CID-CSIC, Jordi Girona 18-26, 08034 Barcelona, Catalonia, Spain.
authors.forename: Eva
authors.initials: E
authors.lastname: Prats
authors.affiliation: Department of Environmental Chemistry, IDAEA-CSIC, Jordi Girona 18-26, 08034 Barcelona, Catalonia, Spain.
authors.forename: Benjamí
authors.initials: B
authors.lastname: Piña
authors.affiliation: Department of Environmental Chemistry, IDAEA-CSIC, Jordi Girona 18-26, 08034 Barcelona, Catalonia, Spain.
authors.forename: Romà
authors.initials: R
authors.lastname: Tauler
mesh_terms: D000818:Animals; D004390:Chlorpyrifos / Q000633:toxicity*; D002853:Chromatography, Liquid; D007306:Insecticides / Q000633:toxicity*; D016018:Least-Squares Analysis; D008297:Male; D013058:Mass Spectrometry; D009132:Muscles / Q000187:drug effects* / Q000378:metabolism; D014874:Water Pollutants, Chemical / Q000633:toxicity*; D015027:Zebrafish / Q000378:metabolism*
chemical_list: D007306:Insecticides; D014874:Water Pollutants, Chemical; D004390:Chlorpyrifos
keywords: Environmental pollution; LC-HRMS; Metabolomics; ROI-MCR-ALS; Zebrafish