title: Combinations of fungal and milling pretreatments for enhancing rice straw biogas production during solid-state anaerobic digestion.
abstract: Rice straw was pretreated by different combinations of physical (milling) and biological (incubation with Pleurotus ostreatus fungus) treatment to improve its biodegradability and biogas production during solid-state anaerobic digestion (SS-AD). Effects of milling (⩽2mm) and incubation time (10, 20 and 30d), on lignin, cellulose, and hemicellulose degradation during fungal pretreatment and methane yield during digestion were assessed by comparison with untreated rice straw. Both incubation time and milling had significant impacts on both lignin removal during fungal pre-treatment and methane yield during digestion. A combination of fungal pretreatment at 30days followed by milling prior to anaerobic digestion resulted in 30.4% lignin removal, the highest selectivity value (the ratio between relative lignin removal and relative cellulose removal) of 4.22, and the highest methane yield of 258L/kgVS. This was equivalent to a 165% increase in methane yield from SS-AD compared to untreated rice straw.
authors.affiliation: College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, China; Department of Agricultural Engineering, Faculty of Agriculture, Suez Canal University, Ismailia 41522, Egypt.
authors.forename: Ahmed M
authors.initials: AM
authors.lastname: Mustafa
authors.affiliation: Department of Civil Engineering, Xi'an Jiaotong-Liverpool University, Suzhou 215123, China.
authors.forename: Tjalfe G
authors.initials: TG
authors.lastname: Poulsen
authors.affiliation: College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, China.
authors.forename: Yihua
authors.initials: Y
authors.lastname: Xia
authors.affiliation: College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, China. Electronic address: kcsheng@zju.edu.cn.
authors.forename: Kuichuan
authors.initials: K
authors.lastname: Sheng
mesh_terms: D001673:Biodegradation, Environmental; D056804:Biofuels / Q000032:analysis*; D008031:Lignin / Q000737:chemistry / Q000378:metabolism; D008697:Methane / Q000032:analysis / Q000378:metabolism; D012275:Oryza / Q000737:chemistry / Q000378:metabolism; D020076:Pleurotus / Q000378:metabolism
chemical_list: D056804:Biofuels; D008031:Lignin; D008697:Methane
keywords: Fungal pretreatment; Incubation time; Methane yield; Milling; Rice straw; Solid-state anaerobic digestion