title: MicroRNA-98 regulates osteogenic differentiation of human bone mesenchymal stromal cells by targeting BMP2.
abstract: To study the effects of microRNA-98 (miR-98) on human bone mesenchymal stromal cells (hBMSCs). The patients undergoing hip arthroplasty were selected by inclusion/exclusion criteria for this study. The extracted hBMSCs were detected of osteogenic differentiation by alizarin red S staining, and of cell phenotype by flow cytometry. Bioinformatics, dual luciferase report, western blotting, RT-PCR and immunoblotting were used in our study. The hBMSCs were divided into miR-98 mimics, miR-98 negative control (NC), miR-98 inhibitors, Mock and miR-98 inhibitors + siBMP2 groups. Human bone mesenchymal stromal cells were extracted and purified in vitro and had specific cytological morphology, surface markers and abilities of self-renewal and differentiation. Compared with the NC group and Mock group, the miR-98 mimics group showed increased miR-98 level while the miR-98 inhibitors group decreased miR-98 level (both P < 0.01). Dual luciferase reporter showed BMP2 was the target gene of miR-98. The levels of mRNA and protein expression of BMP2, protein expression of RUNX2, alkaline phosphatase activity and osteocalcin content significantly decreased in the miR-98 mimics group while increased in the miR-98 inhibitors group and showed no changes in the NC group and Mock group (all P < 0.05). The miR-98 mimics group showed obviously declined stained red particles and the miR-98 inhibitors group showed opposite result. After lowering the expression of miR-98, osteogenic differentiation ability of hBMSCs rose, which was weakened by the transfection with siBMP2. miR-98 may regulate osteogenic differentiation of hBMSCs by targeting BMP2.
authors.affiliation: Department of Orthopedics, The First Hospital of Hebei Medical University, Shijiazhuang, China.
authors.forename: Guo-Ping
authors.initials: GP
authors.lastname: Zhang
authors.affiliation: Medical Physics Department of Basic Medical College of Hebei Medical University, Shijiazhuang, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Department of Orthopedics, The First Hospital of Hebei Medical University, Shijiazhuang, China.
authors.forename: Chao-Hua
authors.initials: CH
authors.lastname: Zhu
authors.affiliation: Department of Orthopedics, The First Hospital of Hebei Medical University, Shijiazhuang, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Lin
authors.affiliation: Department of Orthopedics, The First Hospital of Hebei Medical University, Shijiazhuang, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Wang
authors.affiliation: Department of Orthopedics, The First Hospital of Hebei Medical University, Shijiazhuang, China.
authors.forename: Hai-Jing
authors.initials: HJ
authors.lastname: Zhang
authors.affiliation: Department of Orthopedics, The First Hospital of Hebei Medical University, Shijiazhuang, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Li
authors.affiliation: Department of Orthopedics, The First Hospital of Hebei Medical University, Shijiazhuang, China.
authors.forename: Xiao-Guang
authors.initials: XG
authors.lastname: Yu
authors.affiliation: Department of Orthopedics, The First Hospital of Hebei Medical University, Shijiazhuang, China.
authors.forename: Zhen-Shuan
authors.initials: ZS
authors.lastname: Zhao
authors.affiliation: Department of Orthopedics, The First Hospital of Hebei Medical University, Shijiazhuang, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Dong
authors.affiliation: Department of Orthopedics, The First Hospital of Hebei Medical University, Shijiazhuang, China.
authors.forename: Guo-Bin
authors.initials: GB
authors.lastname: Liu
mesh_terms: D001483:Base Sequence; D015415:Biomarkers / Q000378:metabolism; D001854:Bone Marrow Cells / Q000166:cytology* / Q000378:metabolism; D055396:Bone Morphogenetic Protein 2 / Q000378:metabolism*; D002118:Calcium / Q000378:metabolism; D002454:Cell Differentiation / Q000235:genetics*; D048430:Cell Shape / Q000235:genetics; D005260:Female; D006801:Humans; D008156:Luciferases / Q000378:metabolism; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism*; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D008875:Middle Aged; D010012:Osteogenesis / Q000235:genetics*; D015203:Reproducibility of Results
chemical_list: C525718:BMP2 protein, human; D015415:Biomarkers; D055396:Bone Morphogenetic Protein 2; C546084:MIRN98 microRNA, human; D035683:MicroRNAs; D008156:Luciferases; D002118:Calcium
keywords: bone morphogenetic protein-2; human bone mesenchymal stromal cells; microRNA; microRNA-98; osteogenic differentiation; signal pathway; transcription; translation