title: Improving the encapsulation efficiency and sustained release behaviour of chitosan/β-lactoglobulin double-coated microparticles by palmitic acid grafting.
abstract: Chitosan (CS) was grafted with 0.1 and 0.5% (w/v) palmitic acid (PA) to improve its encapsulation efficiency (EE) and sustained release characteristics when forming CS microparticles. Thereafter, PA-grafted CS (PA-CS) microparticles were coated with denatured β-lactoglobulin (βlg), which forms an outer protective layer. The possibility of hydrophobic interaction with the hydrophobic substances in the CS microparticles increased as the proportion of the grafted PA increased. EE was measured as 64.79, 83.72, and 85.00% for the non-grafted, 0.1, and 0.5% PA-CS microparticles, respectively. In simulated small intestinal conditions, 4.66 and 17.55% of the core material release in the PA-CS microparticles were sustained after 180min by 0.1, and 0.5% PA grafting, respectively. PA grafting enables the sustained release in simulated gastrointestinal fluids by enhancing the hydrophobic interaction between CS and the hydrophobic core material.
authors.affiliation: Department of Food Science and Technology, Sejong University, Republic of Korea. Electronic address: hjyang@sju.ac.kr.
authors.forename: Han-Joo
authors.initials: HJ
authors.lastname: Yang
authors.affiliation: Department of Food Science and Technology, Sejong University, Republic of Korea. Electronic address: sherrene_lps@yahoo.com.sg.
authors.forename: Pei Sia
authors.initials: PS
authors.lastname: Lee
authors.affiliation: Department of Food Science and Technology, Sejong University, Republic of Korea. Electronic address: choohc2002@naver.com.
authors.forename: Jaehyeog
authors.initials: J
authors.lastname: Choe
authors.affiliation: Department of Food Science and Technology, Sejong University, Republic of Korea. Electronic address: sjs007kr@hanmail.net.
authors.forename: Seokjin
authors.initials: S
authors.lastname: Suh
authors.affiliation: Department of Food Science and Technology, Sejong University, Republic of Korea. Electronic address: sanghoonko@sejong.ac.kr.
authors.forename: Sanghoon
authors.initials: S
authors.lastname: Ko
mesh_terms: D055598:Chemical Phenomena; D048271:Chitosan / Q000737:chemistry*; D003692:Delayed-Action Preparations / Q000737:chemistry*; D041981:Gastrointestinal Tract / Q000378:metabolism; D057927:Hydrophobic and Hydrophilic Interactions; D007782:Lactoglobulins / Q000737:chemistry*; D046529:Microscopy, Electron, Transmission; D019308:Palmitic Acid / Q000737:chemistry*; D010316:Particle Size; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D003692:Delayed-Action Preparations; D007782:Lactoglobulins; D019308:Palmitic Acid; D048271:Chitosan
keywords: Chitosan; Grafting; Microparticle; Palmitic acid; Sustained release; β-Lactoglobulin