title: Gadolinium hybrid iron oxide nanocomposites for dual T1- and T2-weighted MR imaging of cell labeling.
abstract: Tracking of cells in biological systems is critically important for monitoring disease treatment, such as in stem cell therapy. This report prepared new types of biocompatible gadolinium hybrid iron oxide (GdIO) nanocomposites, which demonstrated high sensitivity for dual T1- and T2-weighted magnetic resonance imaging (MRI). The GdIO nanocomposites could efficiently label mesenchymal stem cells (MSCs) by incubation for 24 h at a safe dose, as they did not affect the cell viability, proliferation or differentiation capacity. There was high contrast enhancement in the GdIO-labeled stem cells for dual T1- and T2-weighted MR imaging. In addition, the GdIO nanocomposites were injected into adult mouse lateral ventricles, where cells could be labeled to monitor their biological behaviors by MRI. These GdIO nanocomposites with dual-imaging functions are a good platform for cell labeling and other diagnostic applications.
authors.affiliation: Department of Pharmacology, West China School of Preclinical and Forensic Medicine & Collaborative Innovation Center for Biotherapy, West China Hospital, Sichuan University, Chengdu 610041, China. zhou108@163.com.
authors.forename: Yun
authors.initials: Y
authors.lastname: Zeng
authors.affiliation: State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Collaborative Innovation Center for Biotherapy, Sichuan University, Chengdu 610041, China. mi@scu.edu.cn and State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics & Center for Molecular Imaging and Translational Medicine, School of Public Health, Xiamen University, Xiamen 361102, China. gangliu.cmitm@xmu.edu.cn.
authors.forename: Liqin
authors.initials: L
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics & Center for Molecular Imaging and Translational Medicine, School of Public Health, Xiamen University, Xiamen 361102, China. gangliu.cmitm@xmu.edu.cn.
authors.forename: Zijian
authors.initials: Z
authors.lastname: Zhou
authors.affiliation: State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics & Center for Molecular Imaging and Translational Medicine, School of Public Health, Xiamen University, Xiamen 361102, China. gangliu.cmitm@xmu.edu.cn.
authors.forename: Xiaoyong
authors.initials: X
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics & Center for Molecular Imaging and Translational Medicine, School of Public Health, Xiamen University, Xiamen 361102, China. gangliu.cmitm@xmu.edu.cn.
authors.forename: Yang
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics & Center for Molecular Imaging and Translational Medicine, School of Public Health, Xiamen University, Xiamen 361102, China. gangliu.cmitm@xmu.edu.cn.
authors.forename: Junqing
authors.identifier: http://orcid.org/0000-0002-9091-9891
authors.initials: J
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Collaborative Innovation Center for Biotherapy, Sichuan University, Chengdu 610041, China. mi@scu.edu.cn.
authors.forename: Peng
authors.initials: P
authors.lastname: Mi
authors.affiliation: State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics & Center for Molecular Imaging and Translational Medicine, School of Public Health, Xiamen University, Xiamen 361102, China. gangliu.cmitm@xmu.edu.cn.
authors.forename: Gang
authors.initials: G
authors.lastname: Liu
authors.affiliation: Department of Pharmacology, West China School of Preclinical and Forensic Medicine & Collaborative Innovation Center for Biotherapy, West China Hospital, Sichuan University, Chengdu 610041, China. zhou108@163.com.
authors.forename: Liming
authors.initials: L
authors.lastname: Zhou
mesh_terms: D000818:Animals; D003287:Contrast Media; D005290:Ferric Compounds / Q000737:chemistry* / Q000633:toxicity; D005682:Gadolinium / Q000737:chemistry* / Q000633:toxicity; D008279:Magnetic Resonance Imaging / Q000379:methods*; D059630:Mesenchymal Stem Cells / Q000187:drug effects / Q000378:metabolism; D051379:Mice; D053761:Nanocomposites / Q000737:chemistry* / Q000633:toxicity; D013194:Staining and Labeling
chemical_list: D003287:Contrast Media; D005290:Ferric Compounds; C000499:ferric oxide; D005682:Gadolinium