title: Comparison of In-Solution Biorecognition Properties of Aptamers against Ochratoxin A.
abstract: Ochratoxin A (OTA) is a mycotoxin produced as a secondary metabolite by several species ofAspergillusandPenicilliumand frequently found as a natural contaminant in a wide range of food commodities. Novel and robust biorecognition agents for detecting this molecule are required. Aptamers are artificial nucleic acid ligands able to bind with high affinity and specificity to a given target molecule. In the last few years, three separate research groups have selected aptamers for ochratoxin A. While each of these three families of aptamers have been incorporated into various methods for detecting OTA, it is unclear if each aptamer candidate is better suited for a particular application. Here, we perform the first head-to-head comparison of solution-based binding parameters for these groups of aptamers. Based on our results, we provide recommendations for the appropriate choice of aptamer for incorporation into solution-based biorecognition assays and applications.
authors.affiliation: Chemistry Department, Carleton University, 1125 Colonel by Drive, Ottawa, ON K1S 5B6, Canada. mmckeague@gmail.com.
authors.forename: Maureen
authors.initials: M
authors.lastname: McKeague
authors.affiliation: Chemistry Department, Carleton University, 1125 Colonel by Drive, Ottawa, ON K1S 5B6, Canada. rvelunathan@gmail.com.
authors.forename: Ranganathan
authors.initials: R
authors.lastname: Velu
authors.affiliation: Institute of Sciences of Food Production, National Research Council of Italy, via G. Amendola 122/O, Bari 70126, Italy. annalisa.degirolamo@ispa.cnr.it.
authors.forename: Annalisa
authors.initials: A
authors.lastname: De Girolamo
authors.affiliation: Institute of Sciences of Food Production, National Research Council of Italy, via G. Amendola 122/O, Bari 70126, Italy. stefania.valenzano@ispa.cnr.it.
authors.forename: Stefania
authors.initials: S
authors.lastname: Valenzano
authors.affiliation: Institute of Sciences of Food Production, National Research Council of Italy, via G. Amendola 122/O, Bari 70126, Italy. michelangelo.pascale@ispa.cnr.it.
authors.forename: Michelangelo
authors.initials: M
authors.lastname: Pascale
authors.affiliation: Chemistry Department, Carleton University, 1125 Colonel by Drive, Ottawa, ON K1S 5B6, Canada. mckenziesmith@cmail.carleton.ca.
authors.forename: McKenzie
authors.initials: M
authors.lastname: Smith
authors.affiliation: Chemistry Department, Carleton University, 1125 Colonel by Drive, Ottawa, ON K1S 5B6, Canada. maria.derosa@carleton.ca.
authors.forename: Maria C
authors.initials: MC
authors.lastname: DeRosa
mesh_terms: D052157:Aptamers, Nucleotide / Q000737:chemistry*; D015374:Biosensing Techniques; D006046:Gold / Q000737:chemistry; D053768:Metal Nanoparticles / Q000737:chemistry; D009793:Ochratoxins / Q000032:analysis* / Q000737:chemistry; D012996:Solutions
chemical_list: D052157:Aptamers, Nucleotide; D009793:Ochratoxins; D012996:Solutions; C025589:ochratoxin A; D006046:Gold
keywords: aptamer; aptasensor; biorecognition; biosensing; fluorescent assay; microscale thermophoresis; mycotoxins; ochratoxin A