title: Influence of endophytic Bacillus pumilus and EDTA on the phytoextraction of Cu from soil by using Cicer arietinum.
abstract: In developing countries, soil contamination with metals is ubiquitous, which poses a serious threat to the ecosystem. The current study was designed to screen out the nested belongings of Cicer arietinum plants and Bacillus pumilus (KF 875447) in extracting copper (Cu) from contaminated soils. A pot experiment was executed by growing C. arietinum seedlings either inoculated with B. pumilus or uninoculated along with the application of 5 mM ethylenediaminetetraacetic acid (EDTA). Plants were subjected to three different concentrations of Cu (250, 350, and 500 ppm) for 48 days. An increase in Cu uptake was observed in C. arietinum plants inoculated with B. pumilus as compared to uninoculated ones. C. arietinum exhibited improved values for different growth parameters in the presence of B. pumilus, that is, root length (37%), shoot length (31%), whole plant fresh as well as (45%) dry weight (27%), and chlorophyll contents (32%). More than 70% of tolerance index (TI) was observed for plants at 500 ppm Cu treatment. Addition of B. pumilus and EDTA significantly increased metal uptake by C. arietinum up to 19 and 36%, respectively, while the application of B. pumilus and EDTA in combination increased metal accumulation by 41%. The calculated bioaccumulation and translocation factor (TF) revealed that C. arietinum possess phytoextraction potential for Cu, and this ability is significantly improved with application of B. pumilus and EDTA amendments.
authors.affiliation: a Department of Plant Sciences , Faculty of Biological Sciences, Quaid-i-Azam University , Islamabad , Pakistan.
authors.forename: Barkat
authors.initials: B
authors.lastname: Ali
authors.affiliation: a Department of Plant Sciences , Faculty of Biological Sciences, Quaid-i-Azam University , Islamabad , Pakistan.
authors.lastname: Amna
authors.affiliation: b Department of Botany , Government College University , Faisalabad , Pakistan.
authors.forename: Muhammad Tariq
authors.initials: MT
authors.lastname: Javed
authors.affiliation: c Department of Zoology , University of Malakand , Khyber Pakhtunkhwa , Pakistan.
authors.forename: Hazrat
authors.initials: H
authors.lastname: Ali
authors.affiliation: a Department of Plant Sciences , Faculty of Biological Sciences, Quaid-i-Azam University , Islamabad , Pakistan.
authors.forename: Muhammad Farooq Hussain
authors.initials: MF
authors.lastname: Munis
authors.affiliation: a Department of Plant Sciences , Faculty of Biological Sciences, Quaid-i-Azam University , Islamabad , Pakistan.
authors.forename: Hassan Javed
authors.initials: HJ
authors.lastname: Chaudhary
mesh_terms: D000069979:Bacillus pumilus / Q000502:physiology*; D001673:Biodegradation, Environmental; D002614:Chelating Agents / Q000008:administration & dosage / Q000494:pharmacology*; D029863:Cicer / Q000378:metabolism*; D003300:Copper / Q000378:metabolism*; D004492:Edetic Acid / Q000008:administration & dosage / Q000494:pharmacology*; D060026:Endophytes / Q000502:physiology*; D012989:Soil Pollutants / Q000378:metabolism*
chemical_list: D002614:Chelating Agents; D012989:Soil Pollutants; D003300:Copper; D004492:Edetic Acid
keywords: Bacillus pumilus; Cicer arietinum; EDTA; copper; phytoremediation