title: Synthesis and characterization of silver nanoparticles using Bacillus amyloliquefaciens and Bacillus subtilis to control filarial vector Culex pipiens pallens and its antimicrobial activity.
abstract: Culex pipiens pallens are the most common mosquito's vector in Asia. In order to protect the people from diseases, the anti-mosquito population is necessary that uses safe and new bio-pesticides such as bacteria-AgNPs. In our report, we used two kinds of bacteria to synthesize silver nanoparticles to examine the toxic effect on the larvae and pupae of Cx. pipiens pallens and also used as antimicrobial activity. The biosynthesis of AgNPs and its characterization was carried out by UV-Vis spectrophotometry, FTIR, TEM, SEM, and EDX. The larvicidal and pupicidal assays revealed that the lethal concentration LC50values of Bacillus amyloliquefaciens-AgNPs were 0.72 ppm (I), 0.73 ppm (II), 0.69 ppm (III), 1.16 ppm (IV), and 4.18 (Pupae), while LC50values of Bacillus subtilis-AgNPs were 0.60 ppm (I), 0.62 ppm (II), 0.21 ppm (III), 0.28 ppm (IV), and 3.46 ppm (Pupae) after 72 h of exposure. Antibacterial activity test of AgNPs reveals better results against rice pathogenic bacteria than bacteria alone. Thus, bacteria-mediated silver nanoparticles have a rapid effect on vector mosquito and microbial pathogen suggesting savings of energy and resources. Hence, bacteria-AgNPs may be used in the future as an effective weapon to control vector mosquito and harmful bacteria.
authors.affiliation: a Ministry of Agriculture Key Laboratory of Agricultural Entomology , Institute of Insect Sciences, Zhejiang University , Zhejiang , PR China.|b Department of Field Crop Pests , Plant Protection Research Institute, Agricultural Research Centre , Cairo , Egypt.
authors.forename: Hatem
authors.initials: H
authors.lastname: Fouad
authors.affiliation: a Ministry of Agriculture Key Laboratory of Agricultural Entomology , Institute of Insect Sciences, Zhejiang University , Zhejiang , PR China.
authors.forename: Li
authors.initials: L
authors.lastname: Hongjie
authors.affiliation: a Ministry of Agriculture Key Laboratory of Agricultural Entomology , Institute of Insect Sciences, Zhejiang University , Zhejiang , PR China.
authors.forename: Ding
authors.initials: D
authors.lastname: Yanmei
authors.affiliation: a Ministry of Agriculture Key Laboratory of Agricultural Entomology , Institute of Insect Sciences, Zhejiang University , Zhejiang , PR China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Baoting
authors.affiliation: c Department of Crop Protection , University of Bakht Alruda , Ad Douiem , White Nile State , Sudan.
authors.forename: Ahmed
authors.initials: A
authors.lastname: El-Shakh
authors.affiliation: d Department of Chemical Engineering , University of Gujrat , Gujrat , Pakistan.
authors.forename: Ghulam
authors.initials: G
authors.lastname: Abbas
authors.affiliation: a Ministry of Agriculture Key Laboratory of Agricultural Entomology , Institute of Insect Sciences, Zhejiang University , Zhejiang , PR China.
authors.forename: Mo
authors.initials: M
authors.lastname: Jianchu
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000096:biosynthesis / Q000737:chemistry / Q000494:pharmacology; D000069977:Bacillus amyloliquefaciens / Q000378:metabolism* / Q000502:physiology; D001412:Bacillus subtilis / Q000378:metabolism* / Q000502:physiology; D003465:Culex; D007306:Insecticides / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D007814:Larva; D053768:Metal Nanoparticles; D036103:Nanotechnology; D011679:Pupa; D012834:Silver / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology*
chemical_list: D000900:Anti-Bacterial Agents; D007306:Insecticides; D012834:Silver
keywords: Bacillus amyloliquefaciens; Bacillus subtilis; Culex pipiens pallens; Nanoparticles; antibacterial activity; vector mosquito control