title: Tetrodotoxin- and tributyltin-binding abilities of recombinant pufferfish saxitoxin and tetrodotoxin binding proteins of Takifugu rubripes.
abstract: We investigated the ability of recombinant pufferfish saxitoxin and tetrodotoxin binding protein types 1 and 2 of Takifugu rubripes (rTrub.PSTBP1 and rTrub.PSTBP2) to bind to tetrodotoxin (TTX) and tributyltin. Both rTrub.PSTBPs bound to tributyltin in an ultrafiltration binding assay but lost this ability on heat denaturation. In contrast, only rTrub.PSTBP2 bound to TTX even heat denaturation. This result suggests that the amino acid sequence of PSTBP2 may be contributed for its affinity for TTX.
authors.affiliation: Department of Applied Chemistry and Biotechnology, Graduate School of Natural Science and Technology, Okayama University, Tsushimanaka, Okayama 700-8530, Japan.
authors.forename: Hina
authors.initials: H
authors.lastname: Satone
authors.affiliation: Laboratory of Marine Environmental Science, Faculty of Agriculture, Kyushu University, Hakozaki, Fukuoka 812-8581, Japan.
authors.forename: Shohei
authors.initials: S
authors.lastname: Nonaka
authors.affiliation: Laboratory of Insect Genome Science, Graduate School of Bioresource and Bioenvironmental Sciences, Kyushu University, Hakozaki, Fukuoka 812-8581, Japan.
authors.forename: Jae Man
authors.initials: JM
authors.lastname: Lee
authors.affiliation: Laboratory of Marine Environmental Science, Faculty of Agriculture, Kyushu University, Hakozaki, Fukuoka 812-8581, Japan.
authors.forename: Yohei
authors.initials: Y
authors.lastname: Shimasaki
authors.affiliation: Laboratory of Insect Genome Science, Graduate School of Bioresource and Bioenvironmental Sciences, Kyushu University, Hakozaki, Fukuoka 812-8581, Japan.
authors.forename: Takahiro
authors.initials: T
authors.lastname: Kusakabe
authors.affiliation: Department of Biology, Faculty of Science, Kyushu University, 744 Motooka, Nishi-ku, Fukuoka 819-0395, Japan.
authors.forename: Shun-Ichiro
authors.initials: SI
authors.lastname: Kawabata
authors.affiliation: Laboratory of Marine Environmental Science, Faculty of Agriculture, Kyushu University, Hakozaki, Fukuoka 812-8581, Japan. Electronic address: yoshima@agr.kyushu-u.ac.jp.
authors.forename: Yuji
authors.initials: Y
authors.lastname: Oshima
mesh_terms: D000818:Animals; D029941:Fish Proteins / Q000737:chemistry* / Q000302:isolation & purification; D011485:Protein Binding; D011994:Recombinant Proteins / Q000737:chemistry*; D023741:Takifugu; D013779:Tetrodotoxin / Q000737:chemistry*; D014220:Trialkyltin Compounds / Q000737:chemistry*; D014462:Ultrafiltration
chemical_list: D029941:Fish Proteins; D011994:Recombinant Proteins; D014220:Trialkyltin Compounds; D013779:Tetrodotoxin; C011559:tributyltin
keywords: Binding assay; Pufferfish saxitoxin and tetrodotoxin binding proteins; Tetrodotoxin