title: Silkworm larvae plasma (SLP) assay for detection of bacteria: False positives secondary to inflammation in vivo.
abstract: The silkworm larvae plasma (SLP) assay has been developed as a means to detect bacterial peptidoglycan as a surrogate for live bacteria. Here, we present results that indicate that generation of melanin by this assay is not fully reliable as a surrogate marker for bacterial count.
authors.affiliation: Inflammation Immunobiology Section, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, United States.
authors.forename: Michelle
authors.initials: M
authors.lastname: Ma
authors.affiliation: Inflammation Immunobiology Section, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, United States.
authors.forename: Tyler A
authors.initials: TA
authors.lastname: Rice
authors.affiliation: Inflammation Immunobiology Section, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, United States.
authors.forename: Caroline M
authors.initials: CM
authors.lastname: Percopo
authors.affiliation: Inflammation Immunobiology Section, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, United States. Electronic address: hrosenberg@niaid.nih.gov.
authors.forename: Helene F
authors.initials: HF
authors.lastname: Rosenberg
mesh_terms: D000818:Animals; D001419:Bacteria / Q000302:isolation & purification*; D058491:Bacterial Load; D001681:Biological Assay; D015415:Biomarkers / Q000097:blood; D012831:Bombyx / Q000378:metabolism*; D004269:DNA, Bacterial / Q000302:isolation & purification; D005260:Female; D007249:Inflammation / Q000097:blood* / Q000382:microbiology; D048191:Lactiplantibacillus plantarum / Q000302:isolation & purification; D007814:Larva / Q000378:metabolism; D008168:Lung / Q000382:microbiology; D008543:Melanins / Q000097:blood; D051379:Mice; D008810:Mice, Inbred C57BL; D010457:Peptidoglycan / Q000097:blood; D051192:Receptors, Pattern Recognition / Q000819:agonists / Q000378:metabolism; D012680:Sensitivity and Specificity; D051195:Toll-Like Receptor 2 / Q000235:genetics / Q000378:metabolism
chemical_list: D015415:Biomarkers; D004269:DNA, Bacterial; D008543:Melanins; D010457:Peptidoglycan; D051192:Receptors, Pattern Recognition; C493486:Tlr2 protein, mouse; D051195:Toll-Like Receptor 2
keywords: Inflammation; Lactobacillus; Pattern recognition receptors