title: Knock-down of apoptosis inducing factor gene protects endoplasmic reticulum stress-mediated goat granulosa cell apoptosis.
abstract: The apoptosis of granulosa cells is the main cause of follicular atresia, and endoplasmic reticulum (ER) stress is involved in the apoptosis of granulosa cells. Apoptosis inducing factor (AIF) mediates caspase-independent apoptosis and causes chromatin condensation and DNA fragmentation, but its role in ER stress-mediated granulosa cell apoptosis during goat follicular atresia remains largely unknown. The aim of this study was to investigate the function of AIF in the apoptosis of goat granulosa cells mediated by ER stress. The results of immunohistochemical and Western blot analyses demonstrated that AIF was mainly located in granulosa cells, and the expression of AIF significantly increased during follicular atresia. Then, AIF-short hairpin RNA recombinant lentiviral vectors were constructed successfully and transfected into human telomerase reverse transcriptase-goat granulosa cells (hTERT-GGCs). Real-time quantitative polymerase chain reaction and Western blot analysis confirmed that AIF was effectively knocked down in hTERT-GGCs. Flow cytometry results showed that the knockdown of AIF in hTERT-GGCs reduced apoptosis due to serum starvation or thapsigargin (Tg) treatment. In addition, AIF depletion changed the expression of related molecular marker molecules of ER stress under Tg treatment. In conclusion, AIF may serve as a key factor during follicular atresia, and AIF depletion protects ER stress-mediated goat granulosa cell apoptosis.
authors.affiliation: Key Laboratory of Animal Biotechnology of the Ministry of Agriculture, College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, China.
authors.forename: Diqi
authors.initials: D
authors.lastname: Yang
authors.affiliation: Key Laboratory of Animal Biotechnology of the Ministry of Agriculture, College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, China.
authors.forename: Tingting
authors.initials: T
authors.lastname: Jiang
authors.affiliation: Key Laboratory of Animal Biotechnology of the Ministry of Agriculture, College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, China.
authors.forename: Pengfei
authors.initials: P
authors.lastname: Lin
authors.affiliation: Key Laboratory of Animal Biotechnology of the Ministry of Agriculture, College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, China.
authors.forename: Huatao
authors.initials: H
authors.lastname: Chen
authors.affiliation: Key Laboratory of Animal Biotechnology of the Ministry of Agriculture, College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Wang
authors.affiliation: Key Laboratory of Animal Biotechnology of the Ministry of Agriculture, College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, China.
authors.forename: Nan
authors.initials: N
authors.lastname: Wang
authors.affiliation: Key Laboratory of Animal Biotechnology of the Ministry of Agriculture, College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, China.
authors.forename: Fan
authors.initials: F
authors.lastname: Zhao
authors.affiliation: Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, China.
authors.forename: Aihua
authors.initials: A
authors.lastname: Wang
authors.affiliation: Key Laboratory of Animal Biotechnology of the Ministry of Agriculture, College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, China. Electronic address: yapingjin@163.com.
authors.forename: Yaping
authors.initials: Y
authors.lastname: Jin
mesh_terms: D000818:Animals; D051033:Apoptosis Inducing Factor / Q000378:metabolism*; D049109:Cell Proliferation; D002470:Cell Survival; D004721:Endoplasmic Reticulum / Q000502:physiology*; D004791:Enzyme Inhibitors / Q000008:administration & dosage / Q000494:pharmacology; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects / Q000502:physiology; D055785:Gene Knockdown Techniques; D006041:Goats; D006107:Granulosa Cells / Q000502:physiology*; D013312:Stress, Physiological; D019284:Thapsigargin / Q000008:administration & dosage / Q000494:pharmacology
chemical_list: D051033:Apoptosis Inducing Factor; D004791:Enzyme Inhibitors; D019284:Thapsigargin
keywords: Caspase-independent death effector; Follicular atresia; Short hairpin interfering RNA; Unfolded protein response