title: A Protein Interaction Map of the Kalimantacin Biosynthesis Assembly Line.
abstract: The antimicrobial secondary metabolite kalimantacin (also called batumin) is produced by a hybrid polyketide/non-ribosomal peptide system inPseudomonas fluorescensBCCM_ID9359. In this study, the kalimantacin biosynthesis gene cluster is analyzed by yeast two-hybrid analysis, creating a protein-protein interaction map of the entire assembly line. In total, 28 potential interactions were identified, of which 13 could be confirmed further. These interactions include the dimerization of ketosynthase domains, a link between assembly line modules 9 and 10, and a specific interaction between thetrans-acting enoyl reductase BatK and the carrier proteins of modules 8 and 10. These interactions reveal fundamental insight into the biosynthesis of secondary metabolites. This study is the first to reveal interactions in a complete biosynthetic pathway. Similar future studies could build a strong basis for engineering strategies in such clusters.
authors.affiliation: Laboratory of Gene Technology, Department of Biosystems, KU Leuven Leuven, Belgium.
authors.forename: Birgit
authors.initials: B
authors.lastname: Uytterhoeven
authors.affiliation: Laboratory of Gene Technology, Department of Biosystems, KU Leuven Leuven, Belgium.
authors.forename: Thomas
authors.initials: T
authors.lastname: Lathouwers
authors.affiliation: Laboratory of Gene Technology, Department of Biosystems, KU Leuven Leuven, Belgium.
authors.forename: Marleen
authors.initials: M
authors.lastname: Voet
authors.affiliation: Centre for Food and Microbial Technology, Department of Microbial and Molecular Systems, KU Leuven Leuven, Belgium.
authors.forename: Chris W
authors.initials: CW
authors.lastname: Michiels
authors.affiliation: Laboratory of Gene Technology, Department of Biosystems, KU Leuven Leuven, Belgium.
authors.forename: Rob
authors.initials: R
authors.lastname: Lavigne
keywords: antibiotics biosynthesis; batumin; kalimantacin; protein interactions; secondary metabolite; yeast two-hybrid