title: Differentially expressed genes in the testicular tissues of adenylyl cyclase 3 knockout mice.
abstract: Adenylate cyclase 3 (AC3) is an important component of the cyclic adenosine 3',5'-monophosphate (cAMP) signaling pathway and converts adenosine triphosphate into cAMP. Male mice with AC3 deletion (AC3-/-) are sterile. However, the mechanical mechanism remains unclear. By TUNEL staining, we found that cell apoptosis in the testicular tissues of AC3-/-mice increased significantly compared with that in the wild-type (AC3+/+) mice. Differentially expressed genes regulated by AC3 in the testicular tissues were identified by gene chip hybridization. We observed that the expression of 693 genes was altered in the testicular tissues of AC3-/-mice, including 330 up-regulated and 363 down-regulated gene expression with fold changes higher than 2 (≥2) as the standards. Furthermore, part of these differentially expressed genes was verified by the real-time fluorescence quantification PCR and immunofluorescent staining. The expression levels of the genes related to olfactory receptors, cell apoptosis, transcriptional activity, defensive reaction, cell adhesion, cell death, and immunoreactions were significantly altered in the testicular tissues of AC3-/-mice compared with AC3+/+mice. In addition, the corresponding Ca2+, cAMP, and cell adhesion signaling pathways, as well as the signaling pathways related to axon guidance and cell interaction, were altered significantly in the AC3-/-mice. These data would help elucidate the general understanding of the mechanisms underlying the sterility in AC3-/-male mice.
authors.affiliation: College of Life Science, Hebei University, Baoding, Hebei Province 071002, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Zhang
authors.affiliation: College of Life Science, Hebei University, Baoding, Hebei Province 071002, China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Yuan
authors.affiliation: College of Life Science, Hebei University, Baoding, Hebei Province 071002, China.
authors.forename: Qingxiu
authors.initials: Q
authors.lastname: Liu
authors.affiliation: College of Life Science, Hebei University, Baoding, Hebei Province 071002, China.
authors.forename: Dong
authors.initials: D
authors.lastname: Yang
authors.affiliation: College of Public Health, Hebei University, Baoding, Hebei Province 071002, China.
authors.forename: Mingshen
authors.initials: M
authors.lastname: Liu
authors.affiliation: College of Medicine, Hebei University, Baoding, Hebei Province 071002, China.
authors.forename: Limin
authors.initials: L
authors.lastname: Shen
authors.affiliation: College of Life Science, Hebei University, Baoding, Hebei Province 071002, China. Electronic address: zhou66913@163.com.
authors.forename: Yanfen
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: College of Life Science, Hebei University, Baoding, Hebei Province 071002, China. Electronic address: zswang@hbu.edu.cn.
authors.forename: Zhenshan
authors.initials: Z
authors.lastname: Wang
mesh_terms: D000262:Adenylyl Cyclases / Q000172:deficiency* / Q000235:genetics; D000818:Animals; D017209:Apoptosis / Q000235:genetics / Q000502:physiology; D044127:Epigenesis, Genetic; D015870:Gene Expression; D020869:Gene Expression Profiling; D007248:Infertility, Male / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D008297:Male; D051379:Mice; D018345:Mice, Knockout; D020411:Oligonucleotide Array Sequence Analysis; D015398:Signal Transduction / Q000235:genetics; D013737:Testis / Q000378:metabolism* / Q000473:pathology
chemical_list: D000262:Adenylyl Cyclases; C421784:adenylate cyclase 3
keywords: Adenylyl cyclase 3; Differentially expressed genes; Gene chip hybridization; Testicular tissues; cAMP