title: Filipendula ulmaria extracts attenuate cisplatin-induced liver and kidney oxidative stress in rats: In vivo investigation and LC-MS analysis.
abstract: Filipendula ulmaria, known as meadowsweet, is a perennial herb found in wild and cultivated habitats in Europe and Asia. Usage of F. ulmaria in traditional medicine is based on diuretic, astringent, antirheumatic, and anti-inflammatory properties of this plant. Exposure to cisplatin at a dose of 7.5 mg/kg caused significant increase in serum parameters of liver and kidneys function and tissue oxidative stress markers along with some histopathological changes in liver and kidney tissues of experimental rats, as well as high level of genotoxicity. Administration of F. ulmaria extracts in three different concentrations (100, 200, and 400 mg/kg/day) for 10 days resulted in a reduction of oxidative stress in tissues and decrease of serum parameters. Moreover, tested extracts attenuated the genotoxicity of cisplatin in reverse dose-dependent manner. F. ulmaria extracts had no in vitro cytotoxic activity at all applied concentrations (IC50> 50 μg/mL). Tested extracts, rich in polyphenolic compounds, attenuate cisplatin-induced liver and kidney oxidative stress, reduce tissue damage, and enhance the antioxidative status of experimental animals during cisplatin application. Therefore, F. ulmaria extracts may be used as supportive agent for the prevention and amelioration of cisplatin side effects.
authors.affiliation: Department of Chemistry, Faculty of Science, University of Kragujevac, Radoja Domanovića 12, 34000 Kragujevac, Serbia. Electronic address: jkatanic@kg.ac.rs.
authors.forename: Jelena
authors.initials: J
authors.lastname: Katanić
authors.affiliation: Department of Biology and Ecology, Faculty of Science, University of Kragujevac, Radoja Domanovića 12, 34000 Kragujevac, Serbia.
authors.forename: Sanja
authors.initials: S
authors.lastname: Matić
authors.affiliation: Institute of Pharmaceutical Sciences, Department of Pharmacognosy, University of Graz, Universitaetsplatz 4, 8010 Graz, Austria.
authors.forename: Eva-Maria
authors.initials: EM
authors.lastname: Pferschy-Wenzig
authors.affiliation: Institute of Pharmaceutical Sciences, Department of Pharmacognosy, University of Graz, Universitaetsplatz 4, 8010 Graz, Austria.
authors.forename: Nadine
authors.initials: N
authors.lastname: Kretschmer
authors.affiliation: Department of Chemistry, Faculty of Science, University of Kragujevac, Radoja Domanovića 12, 34000 Kragujevac, Serbia.
authors.forename: Tatjana
authors.initials: T
authors.lastname: Boroja
authors.affiliation: Department of Chemistry, Faculty of Science, University of Kragujevac, Radoja Domanovića 12, 34000 Kragujevac, Serbia.
authors.forename: Vladimir
authors.initials: V
authors.lastname: Mihailović
authors.affiliation: Institute of Pathology, Faculty of Medicine, University of Kragujevac, Svetozara Markovića 69, 34000 Kragujevac, Serbia.
authors.forename: Vesna
authors.initials: V
authors.lastname: Stanković
authors.affiliation: Department of Chemistry, Faculty of Science, University of Kragujevac, Radoja Domanovića 12, 34000 Kragujevac, Serbia.
authors.forename: Nevena
authors.initials: N
authors.lastname: Stanković
authors.affiliation: Department of Chemistry, Faculty of Science, University of Kragujevac, Radoja Domanovića 12, 34000 Kragujevac, Serbia.
authors.forename: Milan
authors.initials: M
authors.lastname: Mladenović
authors.affiliation: Department of Biology and Ecology, Faculty of Science, University of Kragujevac, Radoja Domanovića 12, 34000 Kragujevac, Serbia.
authors.forename: Snežana
authors.initials: S
authors.lastname: Stanić
authors.affiliation: Institute for Biological Research "Siniša Stanković", University of Belgrade, Bulevar despota Stefana 142, 11060 Belgrade, Serbia.
authors.forename: Mirjana
authors.initials: M
authors.lastname: Mihailović
authors.affiliation: Institute of Pharmaceutical Sciences, Department of Pharmacognosy, University of Graz, Universitaetsplatz 4, 8010 Graz, Austria.
authors.forename: Rudolf
authors.initials: R
authors.lastname: Bauer
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000633:toxicity; D002470:Cell Survival / Q000187:drug effects; D056486:Chemical and Drug Induced Liver Injury / Q000209:etiology / Q000517:prevention & control*; D002853:Chromatography, Liquid / Q000379:methods; D002945:Cisplatin / Q000633:toxicity*; D004249:DNA Damage / Q000187:drug effects; D031984:Filipendula / Q000737:chemistry*; D007668:Kidney / Q000187:drug effects* / Q000378:metabolism; D007674:Kidney Diseases / Q000139:chemically induced / Q000517:prevention & control*; D008099:Liver / Q000187:drug effects* / Q000378:metabolism; D013058:Mass Spectrometry / Q000379:methods; D018384:Oxidative Stress / Q000187:drug effects*; D010936:Plant Extracts / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D000970:Antineoplastic Agents; D010936:Plant Extracts; D002945:Cisplatin
keywords: Cisplatin; Cytotoxicity; Filipendula ulmaria; Genotoxicity; LC-DAD-MS(n) analysis; Oxidative stress