title: The Oxidative and Inflammatory State in Patients with Acute Renal Graft Dysfunction Treated with Tacrolimus.
abstract: Objective.To determine the oxidative stress/inflammation behavior in patients with/without acute graft dysfunction (AGD) with Tacrolimus.Methods.Cross-sectional study, in renal transplant (RT) recipients (1-yr follow-up). Patients with AGD and without AGD were included. Serum IL-6, TNF-α, 8-isoprostanes (8-IP), and Nitric Oxide (NO) were determined by ELISA; C-reactive protein (CRP) was determined by nephelometry; lipid peroxidation products (LPO) and superoxide dismutase (SOD) were determined by colorimetry.Results.The AGD presentation was at 5.09 ± 3.07 versus 8.27 ± 3.78 months (p< 0.001); CRP >3.19 mg/L was found in 21 versus 19 in the N-AGD group (p= 0.83); TNF-α145.53 ± 18.87 pg/mL versus 125.54 ± 15.92 pg/mL in N-AGD (p= 0.64); IL-6 2110.69 ± 350.97 pg/mL versus 1933.42 ± 235.38 pg/mL in N-AGD (p= 0.13). The LPO were higher in AGD (p= 0.014): 4.10 ± 0.69µM versus 2.41 ± 0.29µM; also levels of 8-IP were higher in AGD 27.47 ± 9.28 pg/mL versus 8.64 ± 1.54 pg/mL (p= 0.01). Serum levels of NO in AGD were lower 138.44 ± 19.20µmol/L versus 190.57 ± 22.04µmol/L in N-AGD (p= 0.042); antioxidant enzyme SOD activity was significantly diminished in AGD with 9.75 ± 0.52 U/mL versus 11.69 ± 0.55 U/mL in N-AGD (p= 0.012).Discussion.Patients with RT present with a similar state of the proinflammatory cytokines whether or not they have AGD. The patients with AGD showed deregulation of the oxidative state with increased LPO and 8-IP and decreased NO and SOD.
authors.affiliation: Institute of Experimental and Clinical Therapeutics, Department of Physiology, University Health Sciences Centre, University of Guadalajara, Guadalajara, JAL, Mexico.
authors.forename: Sandra
authors.initials: S
authors.lastname: Carrillo-Ibarra
authors.affiliation: Department of Nephrology and Transplants, Specialties Hospital, National Occidental Medical Centre, The Mexican Social Security Institute, Guadalajara, JAL, Mexico.
authors.forename: José Ignacio
authors.initials: JI
authors.lastname: Cerrillos-Gutiérrez
authors.affiliation: Department of Nephrology and Transplants, Specialties Hospital, National Occidental Medical Centre, The Mexican Social Security Institute, Guadalajara, JAL, Mexico.
authors.forename: Ariadna
authors.initials: A
authors.lastname: Escalante-Núñez
authors.affiliation: Kidney Diseases Medical Research Unit, Specialties Hospital, National Occidental Medical Centre, The Mexican Social Security Institute, Guadalajara, JAL, Mexico.
authors.forename: Enrique
authors.initials: E
authors.lastname: Rojas-Campos
authors.affiliation: Department of Nephrology and Transplants, Specialties Hospital, National Occidental Medical Centre, The Mexican Social Security Institute, Guadalajara, JAL, Mexico.
authors.forename: Benjamín
authors.initials: B
authors.lastname: Gómez-Navarro
authors.affiliation: Institute of Experimental and Clinical Therapeutics, Department of Physiology, University Health Sciences Centre, University of Guadalajara, Guadalajara, JAL, Mexico.
authors.forename: Sonia
authors.initials: S
authors.lastname: Sifuentes-Franco
authors.affiliation: Institute of Experimental and Clinical Therapeutics, Department of Physiology, University Health Sciences Centre, University of Guadalajara, Guadalajara, JAL, Mexico.
authors.forename: Ernesto Germán
authors.identifier: 0000-0001-7868-667X
authors.initials: EG
authors.lastname: Cardona-Muñoz
authors.affiliation: Institute of Experimental and Clinical Therapeutics, Department of Physiology, University Health Sciences Centre, University of Guadalajara, Guadalajara, JAL, Mexico.
authors.forename: Alejandra Guillermina
authors.identifier: 0000-0002-3635-6135
authors.initials: AG
authors.lastname: Miranda-Díaz
mesh_terms: D000328:Adult; D015415:Biomarkers / Q000378:metabolism; D003710:Demography; D005260:Female; D006801:Humans; D007249:Inflammation / Q000473:pathology*; D018836:Inflammation Mediators / Q000378:metabolism; D016030:Kidney Transplantation / Q000009:adverse effects*; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects; D055031:Primary Graft Dysfunction / Q000188:drug therapy* / Q000209:etiology / Q000473:pathology*; D016559:Tacrolimus / Q000494:pharmacology / Q000627:therapeutic use*
chemical_list: D015415:Biomarkers; D018836:Inflammation Mediators; D016559:Tacrolimus