title: Improved Anticancer Photothermal Therapy Using the Bystander Effect Enhanced by Antiarrhythmic Peptide Conjugated Dopamine-Modified Reduced Graphene Oxide Nanocomposite.
abstract: Despite tremendous efforts toward developing novel near-infrared (NIR)-absorbing nanomaterials, improvement in therapeutic efficiency remains a formidable challenge in photothermal cancer therapy. This study aims to synthesize a specific peptide conjugated polydopamine-modified reduced graphene oxide (pDA/rGO) nanocomposite that promotes the bystander effect to facilitate cancer treatment using NIR-activated photothermal therapy. To prepare a nanoplatform capable of promoting the bystander effect in cancer cells, we immobilized antiarrhythmic peptide 10 (AAP10) on the surface of dopamine-modified rGO (AAP10-pDA/rGO). Our AAP10-pDA/rGO could promote the bystander effect by increasing the expression of connexin 43 protein in MCF-7 breast-cancer cells. Because of its tremendous ability to absorb NIR absorption, AAP10-pDA/rGO offers a high photothermal effect under NIR irradiation. This leads to a massive death of MCF-7 cells via the bystander effect. Using tumor-bearing mice as the model, it is found that NIR radiation effectively ablates breast tumor in the presence of AAP10-pDA/rGO and inhibits tumor growth by ≈100%. Therefore, this research integrates the bystander and photothermal effects into a single nanoplatform in order to facilitate an efficient photothermal therapy. Furthermore, our AAP10-pDA/rGO, which exhibits both hyperthermia and the bystander effect, can prevent breast-cancer recurrence and, therefore, has great potential for future clinical and research applications.
authors.affiliation: Key Laboratory of Nano-Bio Interface, Division of Nanobiomedicine, Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences, Suzhou, 215123, China.
authors.forename: Jiantao
authors.initials: J
authors.lastname: Yu
authors.affiliation: Department of Food and Beverage Management, Taipei College of Maritime Technology, Taipei, 11174, Taiwan.
authors.forename: Yu-Hsin
authors.initials: YH
authors.lastname: Lin
authors.affiliation: Key Laboratory of Nano-Bio Interface, Division of Nanobiomedicine, Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences, Suzhou, 215123, China.
authors.forename: Lingyan
authors.initials: L
authors.lastname: Yang
authors.affiliation: Institute of Bioscience and Biotechnology, National Taiwan Ocean University, Keelung, 20224, Taiwan.
authors.forename: Chih-Ching
authors.initials: CC
authors.lastname: Huang
authors.affiliation: The National Key Laboratory of Shock Wave and Detonation Physics, Institute of Fluid Physics, CAEP, Mianyang, 621900, China.
authors.forename: Liliang
authors.initials: L
authors.lastname: Chen
authors.affiliation: Research Center for Environmental Changes, Academia Sinica, Taipei, 11529, Taiwan.
authors.forename: Wen-Cheng
authors.initials: WC
authors.lastname: Wang
authors.affiliation: Department of Food Science, National Taiwan Ocean University, Keelung, 20224, Taiwan.
authors.forename: Guan-Wen
authors.initials: GW
authors.lastname: Chen
authors.affiliation: Key Laboratory of Nano-Bio Interface, Division of Nanobiomedicine, Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences, Suzhou, 215123, China.
authors.forename: Junyan
authors.initials: J
authors.lastname: Yan
authors.affiliation: Department of Biotechnology, National Formosa University, Yunlin, 63208, Taiwan.
authors.forename: Saranta
authors.initials: S
authors.lastname: Sawettanun
authors.affiliation: Department of Biotechnology, National Formosa University, Yunlin, 63208, Taiwan.
authors.forename: Chia-Hua
authors.initials: CH
authors.lastname: Lin
mesh_terms: D000818:Animals; D024201:Bystander Effect / Q000187:drug effects*; D004298:Dopamine / Q000737:chemistry / Q000494:pharmacology; D005260:Female; D006108:Graphite / Q000737:chemistry / Q000494:pharmacology; D006801:Humans; D006979:Hyperthermia, Induced / Q000379:methods*; D061986:MCF-7 Cells; D051379:Mice; D008819:Mice, Nude; D053761:Nanocomposites / Q000737:chemistry / Q000627:therapeutic use; D009374:Neoplasms, Experimental / Q000473:pathology / Q000628:therapy*; D009842:Oligopeptides / Q000737:chemistry / Q000494:pharmacology; D010789:Phototherapy / Q000379:methods*; D023041:Xenograft Model Antitumor Assays
chemical_list: D009842:Oligopeptides; D006108:Graphite; C029579:antiarrhythmic peptide; D004298:Dopamine
keywords: breast-cancer cells; bystander effect; near-infrared; phototherapy; reduced graphene oxide