title: The aqueous extract ofBrucea javanicasuppresses cell growth and alleviates tumorigenesis of human lung cancer cells by targeting mutated epidermal growth factor receptor.
abstract: As a practical and safe herbal medicine, the seeds ofBrucea javanica(L.) Merr., were used to cure patients suffering from infectious diseases such as malaria. Recent advances revealed that the herb could also be a useful cancer therapy agent. The study demonstrated that aqueousB. javanica(BJ) extract attenuated the growth of human non-small-lung cancer cells bearing mutant L858R/T790M epidermal growth factor receptor (EGFR). The reduced cell viability in H1975 cells was attributed to apoptosis. Transfection of EGFR small hairpin RNA reverted the sensitivities. When nude mice were fed BJ extract, the growth of xenograft tumors, as established by H1975 cells, was suppressed. Additional histological examination and fluorescence analysis of the resected tissues proved that the induced apoptosis mitigated tumor growth. The work proved that the BJ extract exerted its effectiveness by targeting lung cancer cells carrying mutated EGFR while alleviating tumorigenesis. Aqueous BJ extract is a good candidate to overcome drug resistance in patients undergoing target therapy.
authors.affiliation: Department of Life Science, National Taiwan Normal University, Taipei.
authors.forename: Seung-Hun
authors.initials: SH
authors.lastname: Kim
authors.affiliation: Department of Life Science, National Taiwan Normal University, Taipei.
authors.forename: Chun-Yen
authors.initials: CY
authors.lastname: Liu
authors.affiliation: Department of Life Science, National Taiwan Normal University, Taipei.
authors.forename: Po-Wei
authors.initials: PW
authors.lastname: Fan
authors.affiliation: Department of Life Science, National Taiwan Normal University, Taipei.
authors.forename: Chang-Heng
authors.initials: CH
authors.lastname: Hsieh
authors.affiliation: Department of Life Science, National Taiwan Normal University, Taipei.
authors.forename: Hsuan-Yuan
authors.initials: HY
authors.lastname: Lin
authors.affiliation: Brion Research Institute of Taiwan, New Taipei City, Taiwan.
authors.forename: Ming-Chung
authors.initials: MC
authors.lastname: Lee
authors.affiliation: Department of Life Science, National Taiwan Normal University, Taipei.
authors.forename: Kang
authors.initials: K
authors.lastname: Fang
mesh_terms: D000818:Animals; D061067:Antibodies, Monoclonal, Humanized; D032269:Brucea; D002289:Carcinoma, Non-Small-Cell Lung / Q000737:chemistry* / Q000378:metabolism / Q000503:physiopathology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D066246:ErbB Receptors / Q000737:chemistry* / Q000235:genetics; D029001:Herbal Medicine; D006801:Humans; D008175:Lung Neoplasms / Q000737:chemistry / Q000188:drug therapy* / Q000378:metabolism; D051379:Mice; D036702:Quassins / Q000494:pharmacology*; D034741:RNA, Small Interfering / Q000737:chemistry* / Q000378:metabolism; D012639:Seeds / Q000737:chemistry* / Q000378:metabolism
chemical_list: D061067:Antibodies, Monoclonal, Humanized; D036702:Quassins; D034741:RNA, Small Interfering; D066246:ErbB Receptors
keywords: Brucea javanica; apoptosis; epidermal growth factor receptor; herbal medicine; human lung; target therapy