title: Construction and characterization of a pure protein hydrogel for drug delivery application.
abstract: Injectable hydrogels have a variety of applications, including regenerative medicine, tissue engineering and controlled drug delivery. In this paper, we reported on a pure protein hydrogel based on tetrameric recombinant proteins for the potential drug delivery application. This protein hydrogel was formed instantly by simply mixing two recombinant proteins (ULD-TIP1 and ULD-GGGWRESAI) through the specific protein-peptide interaction. The protein hydrogel was characterized by rheology and scanning electron microscopy (SEM). In vitro cytotoxicity test indicated that the developed protein hydrogel had no apparent cytotoxicity against L-929 cells and HCEC cells after 48h incubation. The formed protein hydrogels was gradually degraded after incubation in phosphate buffered solution (PBS, pH=7.4) for a period of 144h study, as indicated by in vitro degradation test. Encapsulation of model drug (sodium diclofenac; DIC) were achieved by simple mixing of drugs with hydrogelator and the entrapped drugs was almost completely released from hydrogels within 24h via a diffusion manner. As a conclusion, the simple and mild preparation procedure and good biocompatibility of protein hydrogel would render its good promising candidate for drug delivery applications.
authors.affiliation: Department of Pharmacology, Xi'an Jiaotong University, Health Science Center, Shanxi, PR China; Institute of Biomedical Engineering, School of Ophthalmology & Optometry and Eye hospital, Wenzhou Medical University, Zhejiang, PR China.
authors.forename: Xu
authors.initials: X
authors.lastname: Xu
authors.affiliation: Institute of Biomedical Engineering, School of Ophthalmology & Optometry and Eye hospital, Wenzhou Medical University, Zhejiang, PR China.
authors.forename: ZhaoKang
authors.initials: Z
authors.lastname: Xu
authors.affiliation: Department of Pharmacology, Xi'an Jiaotong University, Health Science Center, Shanxi, PR China.
authors.forename: XiaoFeng
authors.initials: X
authors.lastname: Yang
authors.affiliation: Department of Pharmacology, Xi'an Jiaotong University, Health Science Center, Shanxi, PR China.
authors.forename: YanHao
authors.initials: Y
authors.lastname: He
authors.affiliation: Department of Pharmacology, Xi'an Jiaotong University, Health Science Center, Shanxi, PR China. Electronic address: linrong@mail.xjtu.edu.cn.
authors.forename: Rong
authors.initials: R
authors.lastname: Lin
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D004337:Drug Carriers / Q000737:chemistry* / Q000633:toxicity; D065546:Drug Liberation; D020100:Hydrogels / Q000737:chemistry*; D051379:Mice; D008958:Models, Molecular; D011487:Protein Conformation; D011994:Recombinant Proteins / Q000737:chemistry* / Q000633:toxicity
chemical_list: D004337:Drug Carriers; D020100:Hydrogels; D011994:Recombinant Proteins
keywords: Drug delivery; Hydrogel; In vitro release; Recombinant proteins