title: The chromatin-remodeling enzyme BRG1 promotes colon cancer progression via positive regulation of WNT3A.
abstract: In this study, we aimed to elucidate the clinical significance and underlying mechanisms of BRG1 in colon cancer. In the clinical analysis, overexpression of BRG1 correlates with colon cancer progression in two cohorts (n = 191 and n = 75). Kaplan-Meier survival analysis revealed that BRG1 is a prognosis predictor for overall survival (P < 0.001) and disease-free survival (P = 0.001). Knocking down BRG1 expression significantly suppressed the proliferation and invasion in colon cancer cells. The expression pattern of WNT3A is consistent with BRG1 in colon cancer tissues and WNT3A expression was inhibited in BRG1 knockdown cells. In addition, restoring WNT3A expression rescues the inhibition of cell proliferation and invasion induced by BRG1. In this study, we demonstrate that BRG1 may contribute to colon cancer progression through upregulating WNT3A expression.
authors.affiliation: Department of General Surgery, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200080,China.
authors.forename: Shengtao
authors.initials: S
authors.lastname: Lin
authors.affiliation: Department of Anal-Colorectal Surgery, General Hospital of Ningxia Medical University, Yinchuan 750004, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Jiang
authors.affiliation: Department of General Surgery, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200080,China.
authors.forename: Ling
authors.initials: L
authors.lastname: Ye
authors.affiliation: Department of General Surgery, Central Hospital of Zi Bo, Zi Bo, Shandong 255000, China.
authors.forename: Zhongbo
authors.initials: Z
authors.lastname: Han
authors.affiliation: Department of General Surgery, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200080,China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Department of General Surgery, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200080,China.
authors.forename: Chenchen
authors.initials: C
authors.lastname: Liu
authors.affiliation: Department of General Surgery, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200080,China.
authors.forename: Chenwei
authors.initials: C
authors.lastname: Yuan
authors.affiliation: Department of General Surgery, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200080,China.
authors.forename: Senlin
authors.initials: S
authors.lastname: Zhao
authors.affiliation: Department of General Surgery, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200080,China.
authors.forename: Jian
authors.initials: J
authors.lastname: Chen
authors.affiliation: Department of General Surgery, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200080,China.
authors.forename: Jingtao
authors.initials: J
authors.lastname: Wang
authors.affiliation: Department of Pathology, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200080, China.
authors.forename: Huamei
authors.initials: H
authors.lastname: Tang
authors.affiliation: Department of Pathology, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200080, China.
authors.forename: Su
authors.initials: S
authors.lastname: Lu
authors.affiliation: Key Lab of Systems Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China.
authors.forename: Liguang
authors.initials: L
authors.lastname: Yang
authors.affiliation: Department of General Surgery, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200080,China.
authors.forename: Xiaoliang
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of General Surgery, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200080,China.
authors.forename: Dongwang
authors.initials: D
authors.lastname: Yan
authors.affiliation: Department of General Surgery, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200080,China.
authors.forename: Zhihai
authors.initials: Z
authors.lastname: Peng
authors.affiliation: Department of General Surgery, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200080,China.
authors.forename: Junwei
authors.initials: J
authors.lastname: Fan
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000818:Animals; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D003110:Colonic Neoplasms / Q000209:etiology* / Q000401:mortality / Q000473:pathology; D004265:DNA Helicases / Q000502:physiology*; D018450:Disease Progression; D005260:Female; D006801:Humans; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008875:Middle Aged; D009361:Neoplasm Invasiveness; D009687:Nuclear Proteins / Q000502:physiology*; D011379:Prognosis; D014157:Transcription Factors / Q000502:physiology*; D060509:Wnt3A Protein / Q000235:genetics*
chemical_list: D009687:Nuclear Proteins; D014157:Transcription Factors; C555510:WNT3A protein, human; D060509:Wnt3A Protein; C084108:SMARCA4 protein, human; D004265:DNA Helicases
keywords: BRG1; WNT3A; colon cancer; progression