title: Crystal structure analysis of a hypothetical protein (MJ0366) from Methanocaldococcus jannaschii revealed a novel topological arrangement of the knot fold.
abstract: The crystal structure of a hypothetical protein MJ0366, derived from Methanocaldococcus jannaschii was solved at 1.9 Å resolution using synchrotron radiation. MJ0366 was crystallized as a monomer and has knot structural arrangement. Intriguingly, the solved structure consists of novel 'KNOT' fold conformation. The 31trefoil knot was observed in the structure. The N-terminal and C-terminal ends did not participate in knot formation.
authors.affiliation: Centre of Advanced Study in Crystallography and Biophysics, University of Madras, Guindy Campus, Chennai, 600025, India.
authors.forename: Viswanathan
authors.initials: V
authors.lastname: Thiruselvam
authors.affiliation: RIKEN Structural Biology Laboratory, Yokohama Institute, RIKEN, 1-7-22 Suehiro-cho, Tsurumi, Yokohama, 230-0045, Japan. Electronic address: kumarevel.thirumananseri@riken.jp.
authors.forename: Thirumananseri
authors.initials: T
authors.lastname: Kumarevel
authors.affiliation: Centre of Advanced Study in Crystallography and Biophysics, University of Madras, Guindy Campus, Chennai, 600025, India.
authors.forename: Ponnuraj
authors.initials: P
authors.lastname: Karthe
authors.affiliation: Department of Biological Sciences, Graduate School of Science, Osaka University, Toyonaka, Osaka, 560-0043, Japan.
authors.forename: Seiki
authors.initials: S
authors.lastname: Kuramitsu
authors.affiliation: RIKEN Structural Biology Laboratory, Yokohama Institute, RIKEN, 1-7-22 Suehiro-cho, Tsurumi, Yokohama, 230-0045, Japan.
authors.forename: Shigeyuki
authors.initials: S
authors.lastname: Yokoyama
authors.affiliation: Centre of Advanced Study in Crystallography and Biophysics, University of Madras, Guindy Campus, Chennai, 600025, India. Electronic address: mnpsy@hotmail.com.
authors.forename: Mondikalipudur Nanjappagounder
authors.initials: MN
authors.lastname: Ponnuswamy
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry* / Q000648:ultrastructure*; D003198:Computer Simulation; D003461:Crystallography; D063747:Methanocaldococcus / Q000378:metabolism*; D008956:Models, Chemical; D008958:Models, Molecular; D011487:Protein Conformation; D017510:Protein Folding
chemical_list: D001426:Bacterial Proteins
keywords: 2EFV; Crystal structure; Hypothetical protein; Knot; Knotted protein; MJ0366; Methanocaldococcus jannaschii; Structural topology