title: A rapid and user-friendly assay to detect the Neutrophil gelatinase-associated lipocalin (NGAL) using up-converting nanoparticles.
abstract: NGAL is a promising novel biomarker for acute kidney injury (AKI) and chronic kidney disease (CKD). More rapid and user-friendly methods are needed for the timely monitoring of NGAL in human urine and serum. UCP technology-based lateral flow assay (UPT-LFA) was developed for rapid, user-friendly and quantitative detection of the NGAL in human serum specimens and urine specimens. Under optimal conditions, the UPT-LFA displayed a rapid response to NGAL with a LOD of 7.68ng/mL and detection range from 7.68 to 1000ng/mL. The UPT-LFA method was compared with commercial immunoturbidimetry (103 urine specimens), and by ELISA (26 serum specimens), respectively. The results demonstrated that the UPT-LFA was consistent with immunoturbidimetry assay, reporting a 97.92% of positive and 92.73% of negative coincidence rates, respectively. Meanwhile, the concordance rate between UPT-LFA and ELISA, as shown by correlative regression analysis, was also high (R2=0.95). The whole assay can be completed within 30min compared to 4h consuming with ELISA. The research implies that, the UPT-LFA provides a potential to be used in point of care testing (POCT) to define early acute kidney injury with advantages of user-friendly and rapid testing, promising this new assay a bright future.
authors.affiliation: College of chemistry and molecular engineering, Nanjing Tech University, Nanjing 211816, China.
authors.forename: Lijiang
authors.initials: L
authors.lastname: Lei
authors.affiliation: College of chemistry and molecular engineering, Nanjing Tech University, Nanjing 211816, China.
authors.forename: Jin
authors.initials: J
authors.lastname: Zhu
authors.affiliation: College of chemistry and molecular engineering, Nanjing Tech University, Nanjing 211816, China.
authors.forename: Gangqiang
authors.initials: G
authors.lastname: Xia
authors.affiliation: College of chemistry and molecular engineering, Nanjing Tech University, Nanjing 211816, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Feng
authors.affiliation: College of chemistry and molecular engineering, Nanjing Tech University, Nanjing 211816, China. Electronic address: hmzhang@njtech.edu.cn.
authors.forename: Hongman
authors.initials: H
authors.lastname: Zhang
authors.affiliation: College of chemistry and molecular engineering, Nanjing Tech University, Nanjing 211816, China. Electronic address: hanyw@njtech.edu.cn.
authors.forename: Yuwang
authors.initials: Y
authors.lastname: Han
mesh_terms: D002846:Chromatography, Affinity / Q000295:instrumentation / Q000379:methods*; D004871:Erbium / Q000737:chemistry; D005453:Fluorescence; D005459:Fluorides / Q000737:chemistry; D006801:Humans; D000071068:Lipocalin-2 / Q000097:blood* / Q000652:urine*; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D046529:Microscopy, Electron, Transmission; D015203:Reproducibility of Results; D013050:Spectrometry, Fluorescence; D015018:Ytterbium / Q000737:chemistry; D015019:Yttrium / Q000737:chemistry
chemical_list: D000071068:Lipocalin-2; D015019:Yttrium; D004871:Erbium; D015018:Ytterbium; D005459:Fluorides
keywords: Acute kidney injury; Lateral flow assay; NGAL; Point of care testing; Up-converting nanoparticles technology