title: Bone marrow mesenchymal stem cells decrease CHOP expression and neuronal apoptosis after spinal cord injury.
abstract: Spinal cord injury (SCI) leads to irreversible neuronal loss and ultimately leads to paralysis. Bone marrow derived mesenchymal stem cells (BMSCs) have been demonstrated to be an effective approach to treat SCI. The present study was designed to investigate the role of BMSCs in rats with spinal cord injury and in oxygen-glucose deprivation (OGD) treated motor neurons. The results demonstrated that BMSCs could improve locomotor function and decrease expression of pro-apoptotic transcription factor C/EBP homologous protein (CHOP) and apoptosis after SCI. Furthermore, co-culture with BMSCs or conditioned medium from BMSCs could also decrease the expression of CHOP and apoptosis in post-OGD motor neurons, supporting that BMSCs exerts protective effects by decreasing the expression of CHOP in injured motor neurons. Our findings provide a potential novel mechanism for BMSCs treatments in patients with SCI.
authors.affiliation: Department of Basic Medicine Sciences, School of Medicine, Zhejiang University, Hangzhou 310058, China.
authors.forename: Chuanlong
authors.initials: C
authors.lastname: Gu
authors.affiliation: Department of Basic Medicine Sciences, School of Medicine, Zhejiang University, Hangzhou 310058, China.
authors.forename: Heyangzi
authors.initials: H
authors.lastname: Li
authors.affiliation: Department of Basic Medicine Sciences, School of Medicine, Zhejiang University, Hangzhou 310058, China.
authors.forename: Chao
authors.initials: C
authors.lastname: Wang
authors.affiliation: Core Facilities, Zhejiang University School of Medicine, Hangzhou 310058, China.
authors.forename: Xinghui
authors.initials: X
authors.lastname: Song
authors.affiliation: School of Medicine, Zhejiang University City College, Hangzhou 310015, China.
authors.forename: Yuemin
authors.initials: Y
authors.lastname: Ding
authors.affiliation: Department of Pharmacology, Hangzhou Medical College, Hangzhou 310053, China.
authors.forename: Mingzhi
authors.initials: M
authors.lastname: Zheng
authors.affiliation: Department of Prosthetics, Stomatology Hospital, School of Medicine, Zhejiang University, Hangzhou 310006, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Liu
authors.affiliation: Department of Basic Medicine Sciences, School of Medicine, Zhejiang University, Hangzhou 310058, China.
authors.forename: Yingying
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Department of Basic Medicine Sciences, School of Medicine, Zhejiang University, Hangzhou 310058, China.
authors.forename: Xiaoming
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Department of Basic Medicine Sciences, School of Medicine, Zhejiang University, Hangzhou 310058, China. Electronic address: wanglinin@zju.edu.cn.
authors.forename: Linlin
authors.initials: L
authors.lastname: Wang
mesh_terms: D000818:Animals; D017209:Apoptosis; D001854:Bone Marrow Cells / Q000166:cytology*; D002470:Cell Survival / Q000502:physiology; D002478:Cells, Cultured; D045164:Mesenchymal Stem Cell Transplantation / Q000379:methods; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D009043:Motor Activity / Q000502:physiology; D017207:Rats, Sprague-Dawley; D020127:Recovery of Function / Q000502:physiology*; D013119:Spinal Cord Injuries / Q000503:physiopathology / Q000628:therapy*; D051742:Transcription Factor CHOP / Q000378:metabolism*
chemical_list: D051742:Transcription Factor CHOP
keywords: Apoptosis; Bone marrow mesenchymal stem cells; C/EBP homology protein; Endoplasmic reticulum stress; Spinal cord injury