title: Defects in the ratio of the dynein isoform, DHC11 in the long-flagella mutants of Chlamydomonas reinhardtii.
abstract: The long-flagella mutants (lf1, lf2, lf3 and lf4) of Chlamydomonas reinhardtii are defective in proteins that are required for the assembly of normal flagella, their phenotype being long flagella. In a previous study, we biophysically characterized these mutants for their waveform patterns, swimming speeds, beat frequencies and correlated these parameters with their flagellar lengths. We found an anomaly in this correlation and set out to explore the underlying molecular significance, if any. The diverse inner dynein isoforms are the flagellar motors that convert the chemical energy of ATP into the mechanical energy of motility; we probed the presence of one of these isoforms (DHC11, which might help in bend initiation) in the lf mutants and compared it with the wild-type. Our studies show that the ratio of DHC11 is defective in the long-flagella mutants of Chlamydomonas reinhardtii.
authors.affiliation: UM-DAE Center for Excellence in Basic Sciences, Kalina Campus, Santacruz (E), Mumbai, India.
authors.forename: Marilyn P
authors.initials: MP
authors.lastname: Sequeira
authors.affiliation: UM-DAE Center for Excellence in Basic Sciences, Kalina Campus, Santacruz (E), Mumbai, India.
authors.forename: Sapna
authors.initials: S
authors.lastname: Sinha
authors.affiliation: UM-DAE Center for Excellence in Basic Sciences, Kalina Campus, Santacruz (E), Mumbai, India.
authors.forename: Mustafa J
authors.initials: MJ
authors.lastname: Motiwalla
authors.affiliation: UM-DAE Center for Excellence in Basic Sciences, Kalina Campus, Santacruz (E), Mumbai, India.
authors.forename: Venkatramanan G
authors.initials: VG
authors.lastname: Rao
authors.affiliation: UM-DAE Center for Excellence in Basic Sciences, Kalina Campus, Santacruz (E), Mumbai, India. Electronic address: jacinta@cbs.ac.in.
authors.forename: Jacinta S
authors.initials: JS
authors.lastname: D'Souza
mesh_terms: D016825:Chlamydomonas reinhardtii / Q000235:genetics* / Q000502:physiology*; D004398:Dyneins / Q000235:genetics*; D009068:Movement; D009154:Mutation; D010940:Plant Proteins / Q000235:genetics*; D020033:Protein Isoforms / Q000235:genetics
chemical_list: D010940:Plant Proteins; D020033:Protein Isoforms; D004398:Dyneins
keywords: Chlamydomonas reinhardtii; DHC11; Dynein isoform; Flagella; lf mutants