title: Interleukin-3 Prevents Cellular Death Induced by Oxidative Stress in HEK293 Cells.
abstract: Interleukin-3 (IL-3) is a well-characterized growth factor in hematopoietic cells, but it is also expressed in other cell types with poorly described functions. Many studies have provided evidence that IL-3 plays an important role in cell survival. We have previously shown that IL-3 is able to increase glucose uptake in HEK293 cells, suggesting that this factor requires sustained glucose metabolism to promote cell survival. In this study, we demonstrate that IL-3 contributes to cell survival under oxidative stress, a prominent feature in the pathophysiology of cancer, diabetes, and neurodegenerative diseases, as well as in the aging process. Our results suggest a molecular mechanism that involves signaling pathways mediated by PI-3k/Akt and Erk. Altogether, these findings show an important role for IL-3 in supporting the viability of non-hematopoietic systems. J. Cell. Biochem. 118: 1330-1340, 2017. © 2016 Wiley Periodicals, Inc.
authors.affiliation: Facultad de Ciencias, Instituto de Bioquímica y Microbiología, Valdivia, Chile.
authors.forename: Camila
authors.initials: C
authors.lastname: López
authors.affiliation: Facultad de Ciencias, Instituto de Bioquímica y Microbiología, Valdivia, Chile.
authors.forename: Patricia
authors.initials: P
authors.lastname: Zamorano
authors.affiliation: Facultad de Ciencias, Instituto de Bioquímica y Microbiología, Valdivia, Chile.
authors.forename: Stefanie
authors.initials: S
authors.lastname: Teuber
authors.affiliation: Facultad de Ciencias, Instituto de Bioquímica y Microbiología, Valdivia, Chile.
authors.forename: Mónica
authors.initials: M
authors.lastname: Salas
authors.affiliation: Facultad de Medicina, Instituto de Microbiología Clínica, Valdivia, Los Ríos, Chile.|Center for Interdisciplinary Studies on the Nervous System (CISNe), Universidad Austral de Chile, Valdivia, Chile.
authors.forename: Carola
authors.initials: C
authors.lastname: Otth
authors.affiliation: Facultad de Ciencias Veterinarias, Instituto de Farmacología, Universidad Austral de Chile, Valdivia, Los Ríos, Chile.
authors.forename: María A
authors.initials: MA
authors.lastname: Hidalgo
authors.affiliation: Facultad de Ciencias, Instituto de Bioquímica y Microbiología, Valdivia, Chile.
authors.forename: Ilona
authors.initials: I
authors.lastname: Concha
authors.affiliation: Facultad de Ciencias, Instituto de Bioquímica y Microbiología, Valdivia, Chile.|Center for Interdisciplinary Studies on the Nervous System (CISNe), Universidad Austral de Chile, Valdivia, Chile.
authors.forename: Angara
authors.initials: A
authors.lastname: Zambrano
mesh_terms: D016923:Cell Death; D002470:Cell Survival / Q000187:drug effects; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism; D005947:Glucose / Q000378:metabolism*; D057809:HEK293 Cells; D006801:Humans; D006861:Hydrogen Peroxide / Q000009:adverse effects*; D007377:Interleukin-3 / Q000378:metabolism*; D018384:Oxidative Stress; D019869:Phosphatidylinositol 3-Kinases / Q000378:metabolism; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects
chemical_list: C508596:IL3 protein, human; D007377:Interleukin-3; D006861:Hydrogen Peroxide; D019869:Phosphatidylinositol 3-Kinases; D051057:Proto-Oncogene Proteins c-akt; D048049:Extracellular Signal-Regulated MAP Kinases; D005947:Glucose
keywords: CELL SURVIVAL; Erk; IL-3; OXIDATIVE STRESS; PI3k/Akt