title: Twig pre-harvest temperature significantly influences effective cryopreservation of Vaccinium dormant buds.
abstract: Cryopreservation of temperate woody-plant material by dormant buds is less expensive than using shoot tips isolated from tissue cultured plants; however currently, dormant buds are used only for preservation of selected temperate tree and shrub species. Using dormant buds could be an efficient strategy for long-term preservation of blueberry (Vaccinium L.) genetic resources. In this study, viability of V. hybrid 'Northsky' (PI 554943) dormant buds was evaluated at 30 harvest dates over three consecutive fall/winter seasons to determine the optimal harvest time that promotes high post cryopreservation viability. Twigs with dormant buds were cut into 70 mm segments containing at least two nodes, desiccated, slowly cooled, stored in liquid nitrogen vapor and tested for post-cryopreservation regrowth. The highest regrowth of cryopreserved dormant buds was observed for buds harvested in mid-December and during the first half of January. Pearson's correlation coefficients were computed to evaluate the association between bud characteristics and viability at harvest date and logistic regression models were fit to test the ability of twig characteristics and temperatures to predict post cryopreservation bud viability. Post-cryopreservation viability was negatively correlated (p < 0.05) with average minimum, maximum and daily mean temperature preceding the bud harvest but was not correlated with the dormant bud initial and end moisture content, twig diameter, the number of dormant buds/cm of twig length and the number of days in desiccation. Regression tree analysis suggested post-cryopreservation viability to be between 52 and 80% for dormant buds harvested after a 10 day average maximum air temperature of <11.2 °C. Pre-harvest air temperature was a significant indicator of optimal dormant bud harvest time to produce adequate viability for long term preservation of blueberry genetic resources.
authors.affiliation: Plant and Animal Genetic Resources Preservation Unit, National Laboratory for Genetic Resources Preservation, USDA-ARS, Fort Collins, CO 80521, USA. Electronic address: maria.jenderek@ars.usda.gov.
authors.forename: Maria M
authors.initials: MM
authors.lastname: Jenderek
authors.affiliation: Plant and Animal Genetic Resources Preservation Unit, National Laboratory for Genetic Resources Preservation, USDA-ARS, Fort Collins, CO 80521, USA.
authors.forename: Justin D
authors.initials: JD
authors.lastname: Tanner
authors.forename: Barbara D
authors.initials: BD
authors.lastname: Ambruzs
authors.affiliation: Plains Area, USDA-ARS, Fort Collins, CO 80521, USA.
authors.forename: Mark
authors.initials: M
authors.lastname: West
authors.affiliation: National Clonal Repository, USDA-ARS, Corvallis, OR 97333, USA.
authors.forename: Joseph D
authors.initials: JD
authors.lastname: Postman
authors.affiliation: National Clonal Repository, USDA-ARS, Corvallis, OR 97333, USA.
authors.forename: Kim E
authors.initials: KE
authors.lastname: Hummer
mesh_terms: D015925:Cryopreservation / Q000379:methods*; D003890:Desiccation; D018520:Plant Shoots / Q000254:growth & development*; D018547:Plant Stems / Q000254:growth & development*; D012621:Seasons; D013696:Temperature; D029796:Vaccinium / Q000254:growth & development*
keywords: Blueberry; Gene banking; Post cryopreservation regrowth; Regression models