title: A New Antifungal Isocoumarin from The Endophytic FungusTrichodermaSp. 09 ofMyoporum bontioidesA. Gray.
abstract: BACKGROUND
  Myoporum bontioidesA. Gray is a commonly used medicinal plant in China. Recently, the chemical and bioactive investigations to the endophytic fungi of this plant have led to several new compounds with antimicrobial and cytotoxic activities. To find out more active molecules, the metabolites of an endophytic fungus,Trichodermasp. 09 from the root ofMyoporum bontioideswere investigated.
  
  
  METHODS
  The metabolites were isolated by column chromatography on silica gel, and their structures were elucidated on the basis of spectroscopic analysis[one-dimensional (1D), two-dimensional (2D)-nuclear magnetic resonance (NMR), Mass spectrometry (MS)], and by comparison with the published data. The dilution method was used for the evaluation of antifungal activity.
  
  
  RESULTS
  Four metabolites were isolated and identified as: dichlorodiaportinolide (1), dichlorodiaportin (2), diaportinol (3), and diaportin (4). Compounds1and2showed weak to high antifungal activities againstColletotrichum musae(Berk. and M. A. Curtis) Arx and Rhizoctonia solani Kühn, as compared with the positive control.
  
  
  CONCLUSIONS
  Compound1was a new isocoumarin being worthy of consideration for the development and research of antifungal agents.
  
  
  CONCLUSIONS
  A new isocoumarin named dichlorodiaportinolide, along with dichlorodiaportin, diaportinol, and diaportin were isolated from the endophytic fungusTrichodermasp. 09 of the root ofMyoporum bontioides.Dichlorodiaportinolide and dichlorodiaportin showed weak to high antifungal activities againstmusae and R. solani(MIC values from 6.25 to 150 μg/mL).Dichlorodiaportinolide and dichlorodiaportin were inactive toP. italicandF. graminearum(MIC values > 200 μg/mL).Abbreviations used:IR: Infrared Radiation, HR-ESI-MS: High resolution electrospray ionization mass spectroscopy, LCMS-IT-TOF: Liquid chromatography mass spectroscopy-Ion trap-Time-of-flight, UV: Ultraviolet-visible, HMBC: Heteronuclear multiple bond correlation, NOE: Nuclear Overhauser effect.
authors.affiliation: Department of Applied Chemistry, College of Materials and Energy (Formerly College of Science), South China Agricultural University, Guangzhou, China.
authors.forename: Wensheng
authors.initials: W
authors.lastname: Li
authors.affiliation: Department of Applied Chemistry, College of Materials and Energy (Formerly College of Science), South China Agricultural University, Guangzhou, China.
authors.forename: Jiaxin
authors.initials: J
authors.lastname: Xu
authors.affiliation: Department of Applied Chemistry, College of Materials and Energy (Formerly College of Science), South China Agricultural University, Guangzhou, China.
authors.forename: Fenqi
authors.initials: F
authors.lastname: Li
authors.affiliation: Department of Applied Chemistry, College of Materials and Energy (Formerly College of Science), South China Agricultural University, Guangzhou, China.
authors.forename: Li
authors.initials: L
authors.lastname: Xu
authors.affiliation: Department of Applied Chemistry, College of Materials and Energy (Formerly College of Science), South China Agricultural University, Guangzhou, China.
authors.forename: Chunyuan
authors.initials: C
authors.lastname: Li
keywords: Antifungal activity; Myoporum bontioides; isocoumarin; trichoderma sp. 09