title: A lysosome targetable luminescent bioprobe based on a europium β-diketonate complex for cellular imaging applications.
abstract: Herein, we report a novel lysosome targetable luminescent bioprobe derived from a europium coordination compound, namely Eu(pfphOCH3IN)3(DDXPO) 4 [where HpfphOCH3IN = 4,4,5,5,5-pentafluoro-3-hydroxy-1-(1-(4-methoxyphenyl)-1H-indol-3-yl)pent-2-en-1-one and DDXPO = 4,5-bis(diphenylphosphino)-9,9-dimethylxanthene oxide]. Notably, the newly designed europium complex exhibits significant quantum yield (Φoverall= 25 ± 3%) and5D0excited state lifetime (τ = 398 ± 3 μs) values under physiological pH (7.2) conditions when excited at 405 nm. Hence the developed europium complex has been evaluated for live cell imaging applications using mouse pre-adipocyte cell lines (3T3L1). Colocalization studies of the designed bio-probe with commercial Lysosome-GFP in 3T3L1 cells demonstrated the specific localization of the probe in the lysosome with a high colocalization coefficient (A = 0.83). Most importantly, the developed bioprobe exhibits good cell permeability, photostability and non-cytotoxicity.
authors.affiliation: AcSIR-Academy of Scientific & Innovative Research, CSIR-NIIST Campus, Thiruvananthapuram, India and Materials Science and Technology Division, National Institute for Interdisciplinary Science and Technology (NIIST), Council of Scientific and Industrial Research (CSIR), Thiruvananthapuram-695 019, India. mlpreddy55@gmail.com.
authors.forename: T M
authors.initials: TM
authors.lastname: George
authors.affiliation: Cardiovascular Diseases and Diabetes Biology Lab, Rajiv Gandhi Centre for Biotechnology, Thiruvananthapuram, India.
authors.forename: Mahesh S
authors.initials: MS
authors.lastname: Krishna
authors.affiliation: AcSIR-Academy of Scientific & Innovative Research, CSIR-NIIST Campus, Thiruvananthapuram, India and Materials Science and Technology Division, National Institute for Interdisciplinary Science and Technology (NIIST), Council of Scientific and Industrial Research (CSIR), Thiruvananthapuram-695 019, India. mlpreddy55@gmail.com.
authors.forename: M L P
authors.initials: ML
authors.lastname: Reddy
mesh_terms: D041721:3T3-L1 Cells; D000818:Animals; D056831:Coordination Complexes / Q000032:analysis / Q000737:chemistry*; D005063:Europium / Q000032:analysis / Q000737:chemistry*; D054879:Halogenation; D007211:Indoles / Q000032:analysis / Q000737:chemistry; D008024:Ligands; D049408:Luminescent Agents / Q000032:analysis / Q000737:chemistry*; D008247:Lysosomes / Q000648:ultrastructure*; D051379:Mice; D018613:Microscopy, Confocal / Q000379:methods; D061848:Optical Imaging / Q000379:methods*; D014966:Xanthenes / Q000032:analysis / Q000737:chemistry
chemical_list: C545289:4,5-bis(diphenylphosphino)-9,9-dimethylxanthene oxide; D056831:Coordination Complexes; D007211:Indoles; D008024:Ligands; D049408:Luminescent Agents; D014966:Xanthenes; D005063:Europium