title: Comparative Genomic Analysis ofDelftia tsuruhatensisMTQ3 and the Identification of Functional NRPS Genes for Siderophore Production.
abstract: Plant growth-promoting rhizobacteria (PGPR) are a group of rhizosphere bacteria that promote plant growth.Delftia tsuruhatensisMTQ3 is a member of PGPR that produces siderophores. The draft genome sequence of MTQ3 has been reported. Here, we analyzed the genome sequence of MTQ3 and performed a comparative genome analysis of four sequencedDelftiastrains, revealing genetic relationships among these strains. In addition, genes responsible for bacteriocin and nonribosomal peptide synthesis were detected in the genomes of each strain. To reveal the functions of NRPS genes in siderophore production inD. tsuruhatensisMTQ3, three NRPS genes were knocked out to obtain the three mutants MTQ3-Δ1941, MTQ3-Δ1945, and MTQ3-Δ1946, which were compared with the wild-type strain. In qualitative and quantitative analyses using CAS assay, the mutants failed to produce siderophores. Accordingly, the NRPS genes in MTQ3 were functionally related to siderophore production. These results clarify one mechanism by which plant growth is promoted in MTQ3 and have important applications in agricultural production.
authors.affiliation: College of Life Sciences/Shandong Key Laboratory of Agricultural Microbiology, Shandong Agricultural University, Tai'an, China.
authors.forename: Haimeng
authors.initials: H
authors.lastname: Guo
authors.affiliation: College of Life Sciences/Shandong Key Laboratory of Agricultural Microbiology, Shandong Agricultural University, Tai'an, China.
authors.forename: Yanan
authors.initials: Y
authors.lastname: Yang
authors.affiliation: College of Life Sciences/Shandong Key Laboratory of Agricultural Microbiology, Shandong Agricultural University, Tai'an, China.
authors.forename: Kai
authors.initials: K
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Nutrition Resources Integrated Utilization, Linyi, China.
authors.forename: Wenfeng
authors.initials: W
authors.lastname: Xu
authors.affiliation: GENEWIZ, Suzhou, China.
authors.forename: Jianyong
authors.initials: J
authors.lastname: Gao
authors.affiliation: GENEWIZ, Suzhou, China.
authors.forename: Hairong
authors.initials: H
authors.lastname: Duan
authors.affiliation: College of Life Sciences/Shandong Key Laboratory of Agricultural Microbiology, Shandong Agricultural University, Tai'an, China.
authors.forename: Binghai
authors.initials: B
authors.lastname: Du
authors.affiliation: College of Life Sciences/Shandong Key Laboratory of Agricultural Microbiology, Shandong Agricultural University, Tai'an, China.
authors.forename: Yanqin
authors.identifier: 0000-0002-3276-9143
authors.initials: Y
authors.lastname: Ding
authors.affiliation: College of Life Sciences/Shandong Key Laboratory of Agricultural Microbiology, Shandong Agricultural University, Tai'an, China.
authors.forename: Chengqiang
authors.identifier: 0000-0003-0222-4094
authors.initials: C
authors.lastname: Wang
mesh_terms: D042742:Delftia / Q000235:genetics* / Q000378:metabolism*; D055786:Gene Knockout Techniques; D016680:Genome, Bacterial / Q000235:genetics*; D010455:Peptides / Q000235:genetics / Q000378:metabolism*; D010802:Phylogeny; D017262:Siderophores / Q000235:genetics / Q000378:metabolism*
chemical_list: D010455:Peptides; D017262:Siderophores