title: miR-30e-5p and miR-15a Synergistically Regulate Fatty Acid Metabolism in Goat Mammary Epithelial Cells via LRP6 and YAP1.
abstract: MicroRNA (miRNA) regulates the expression of genes and influences a series of biological processes, including fatty acid metabolism. We screened the expression of miRNA in goat mammary glands during peak-lactation and non-lactating ("dry") periods, and performed an in vitro study with goat mammary epithelial cells (GMEC) prior to sequencing analysis. Results illustrated that miR-30e-5p and miR-15a were highly expressed. Utilizing a luciferase reporter assay and Western blot, low-density lipoprotein receptor-related protein 6 (LRP6) andYes associated protein 1(YAP1) genes were demonstrated to be a target of miR-30e-5p and miR-15a in GMEC. Moreover, we demonstrated that the overexpression of miR-30e-5p and miR-15a in GMEC promoted fat metabolism while their knockdown impaired fat metabolism. These findings extend the discovery of a key role of miR-30e-5p and miR-15a in mediating adipocyte differentiation by suggesting a role in promoting milk fat synthesis. In conclusion, our findings indicate that miR-30e-5p, together with miR-15a, represses expression ofLRP6and promotes fat metabolism in GMEC. The data expanded our knowledge on the function of miRNAs in milk fat metabolism and synthesis in ruminant mammary cells.
authors.affiliation: Shanxi Key Laboratory of Molecular Biology for Agriculture, College of Animal Science and Technology, Northwest A&F University, Yangling 712100, Shaanxi, China. chenzhijerom@163.com.
authors.forename: Zhi
authors.initials: Z
authors.lastname: Chen
authors.affiliation: College of Life Sciences, Shenzhen University, Shenzhen 518060, China. 18938944369@163.com.
authors.forename: Huiling
authors.initials: H
authors.lastname: Qiu
authors.affiliation: College of Life Sciences, Shenzhen University, Shenzhen 518060, China. wind2596358@yahoo.com.
authors.forename: Liuan
authors.initials: L
authors.lastname: Ma
authors.affiliation: Shanxi Key Laboratory of Molecular Biology for Agriculture, College of Animal Science and Technology, Northwest A&F University, Yangling 712100, Shaanxi, China. luojun@nwsuaf.edu.cn.
authors.forename: Jun
authors.initials: J
authors.lastname: Luo
authors.affiliation: Shanxi Key Laboratory of Molecular Biology for Agriculture, College of Animal Science and Technology, Northwest A&F University, Yangling 712100, Shaanxi, China. luckysunshuang@126.com.
authors.forename: Shuang
authors.initials: S
authors.lastname: Sun
authors.affiliation: College of Life Sciences, Shenzhen University, Shenzhen 518060, China. kangkang@szu.edu.cn.
authors.forename: Kang
authors.initials: K
authors.lastname: Kang
authors.affiliation: College of Life Sciences, Shenzhen University, Shenzhen 518060, China. dmgou@hotmail.com.
authors.forename: Deming
authors.initials: D
authors.lastname: Gou
authors.affiliation: Mammalian NutriPhysioGenomics, Department of Animal Sciences, Division of Nutritional Sciences, University of Illinois, Urbana, IL 61801, USA. jloor@illinois.edu.
authors.forename: Juan J
authors.initials: JJ
authors.lastname: Loor
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000235:genetics* / Q000378:metabolism; D000818:Animals; D004847:Epithelial Cells / Q000378:metabolism; D005227:Fatty Acids / Q000235:genetics / Q000378:metabolism*; D005260:Female; D005786:Gene Expression Regulation; D006041:Goats / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D060488:Low Density Lipoprotein Receptor-Related Protein-6 / Q000235:genetics* / Q000378:metabolism; D008321:Mammary Glands, Animal / Q000166:cytology / Q000378:metabolism*; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D015854:Up-Regulation
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D005227:Fatty Acids; D060488:Low Density Lipoprotein Receptor-Related Protein-6; D035683:MicroRNAs
keywords: LRP6; YAP1; fat metabolism; miR-15a; miR-30e-5p