title: Autoantibody Response to ZRF1 and KRR1 SEREX Antigens in Patients with Breast Tumors of Different Histological Types and Grades.
abstract: Purpose. To investigate a frequency of antibody response to SEREX-identified medullary breast carcinoma autoantigens ZRF1 and KRR1 in sera of breast cancer patients taking into account clinical and molecular characteristics of tumors for opening of new perspectives in creation of minimally invasive immunological tests for cancer diagnostics.Methods. Enzyme-linked immunosorbent assay and bioinformatics analysis.Results. Increased frequency of antibody response was found in sera of breast cancer patients to ZRF and KRR1 antigens. The antibody response to these antigens was higher in sera of patients with invasive ductal carcinoma than in sera of patients with other histological types of breast tumors. Moreover, more frequent antibody response to ZRF antigen was found in sera of patients with less aggressive tumors. The sequence analysis of ZRF1 antigen SEREX clones obtained from cDNA libraries of different tumors demonstrates that they encode different protein isoforms.Conclusion. Tumor-associated antigens KRR1 and ZRF1 and their cognate autoantibodies could be considered as potential molecular markers of breast cancer which need to be further investigated.
authors.affiliation: Department of Cell Signaling, Institute of Molecular Biology and Genetics NASU, Kyiv, Ukraine.
authors.forename: Lada
authors.identifier: 0000-0001-9489-0072
authors.initials: L
authors.lastname: Dyachenko
authors.affiliation: Institute of Fundamental Medicine and Biology, Kazan Federal University, Kazan, Russia.
authors.forename: Kristina
authors.identifier: 0000-0001-7360-1948
authors.initials: K
authors.lastname: Havrysh
authors.affiliation: Department of Cell Signaling, Institute of Molecular Biology and Genetics NASU, Kyiv, Ukraine.
authors.forename: Anita
authors.initials: A
authors.lastname: Lytovchenko
authors.affiliation: Breast Cancer Department, National Cancer Institute, Kyiv, Ukraine.
authors.forename: Irina
authors.initials: I
authors.lastname: Dosenko
authors.affiliation: Department of Oncological Pathology, Dnipropetrovsk Regional Center of Pathology, Dnipropetrovsk, Ukraine.
authors.forename: Stepan
authors.initials: S
authors.lastname: Antoniuk
authors.affiliation: Department of Cell Signaling, Institute of Molecular Biology and Genetics NASU, Kyiv, Ukraine.
authors.forename: Valeriy
authors.identifier: 0000-0003-1839-3335
authors.initials: V
authors.lastname: Filonenko
authors.affiliation: Department of Cell Signaling, Institute of Molecular Biology and Genetics NASU, Kyiv, Ukraine; Institute of Fundamental Medicine and Biology, Kazan Federal University, Kazan, Russia.
authors.forename: Ramziya
authors.identifier: 0000-0002-2547-2843
authors.initials: R
authors.lastname: Kiyamova
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000951:Antigens, Neoplasm / Q000097:blood* / Q000235:genetics / Q000276:immunology; D001323:Autoantibodies / Q000097:blood*; D001483:Base Sequence; D014408:Biomarkers, Tumor / Q000097:blood* / Q000276:immunology; D001943:Breast Neoplasms / Q000097:blood / Q000276:immunology* / Q000473:pathology; D018270:Carcinoma, Ductal, Breast / Q000097:blood / Q000276:immunology / Q000473:pathology; D018275:Carcinoma, Lobular / Q000097:blood / Q000276:immunology / Q000473:pathology; D018276:Carcinoma, Medullary / Q000097:blood / Q000276:immunology / Q000473:pathology; D016022:Case-Control Studies; D004268:DNA-Binding Proteins / Q000097:blood* / Q000235:genetics / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005500:Follow-Up Studies; D015723:Gene Library; D006801:Humans; D008875:Middle Aged; D018832:Molecular Chaperones; D060787:Neoplasm Grading; D009367:Neoplasm Staging; D028861:Nuclear Pore Complex Proteins / Q000097:blood / Q000235:genetics / Q000276:immunology; D015513:Oncogene Proteins / Q000097:blood* / Q000235:genetics / Q000276:immunology; D011379:Prognosis; D016601:RNA-Binding Proteins / Q000097:blood / Q000235:genetics / Q000276:immunology; D055815:Young Adult
chemical_list: C119724:AGFG1 protein, human; D000951:Antigens, Neoplasm; D001323:Autoantibodies; D014408:Biomarkers, Tumor; D004268:DNA-Binding Proteins; C414672:DNAJC2 protein, human; D018832:Molecular Chaperones; D028861:Nuclear Pore Complex Proteins; D015513:Oncogene Proteins; D016601:RNA-Binding Proteins