title: Cytosolic iron-sulfur cluster transfer-a proposed kinetic pathway for reconstitution of glutaredoxin 3.
abstract: Iron-sulfur (Fe-S) clusters are ubiquitously conserved and play essential cellular roles. The mechanism of Fe-S cluster biogenesis involves multiple proteins in a complex pathway. Cluster biosynthesis primarily occurs in the mitochondria, but key Fe-S proteins also exist in the cytosol. One such protein, glutaredoxin 3 (Grx3), is involved in iron regulation, sensing, and mediating [2Fe-2S] cluster delivery to cytosolic protein targets, but the cluster donor for cytosolic Grx3 has not been elucidated. Herein, we delineate the kinetic transfer of [2Fe-2S] clusters into Grx3 from potential cytosolic carrier/scaffold proteins, IscU and Nfu, to evaluate a possible model for Grx3 reconstitution in vivo.
authors.affiliation: Department of Chemistry and Biochemistry, The Ohio State University, Columbus, OH, USA.|The Ohio State Biochemistry Program, The Ohio State University, Columbus, OH, USA.
authors.forename: Christine
authors.initials: C
authors.lastname: Wachnowsky
authors.affiliation: Department of Chemistry and Biochemistry, The Ohio State University, Columbus, OH, USA.|The Biophysics Graduate Program, The Ohio State University, Columbus, OH, USA.
authors.forename: Insiya
authors.initials: I
authors.lastname: Fidai
authors.affiliation: Department of Chemistry and Biochemistry, The Ohio State University, Columbus, OH, USA.|The Ohio State Biochemistry Program, The Ohio State University, Columbus, OH, USA.|The Biophysics Graduate Program, The Ohio State University, Columbus, OH, USA.
authors.forename: James A
authors.initials: JA
authors.lastname: Cowan
mesh_terms: D001059:Apoproteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D001692:Biological Transport; D002352:Carrier Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D003600:Cytosol / Q000737:chemistry / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D006801:Humans; D007501:Iron / Q000737:chemistry* / Q000378:metabolism; D007506:Iron-Sulfur Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D007700:Kinetics; D010088:Oxidoreductases / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000737:chemistry / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D013455:Sulfur / Q000737:chemistry* / Q000378:metabolism; D020124:Thermotoga maritima / Q000737:chemistry / Q000378:metabolism
chemical_list: D001059:Apoproteins; D001426:Bacterial Proteins; D002352:Carrier Proteins; C404105:GLRX3 protein, human; C506160:ISCU protein, human; D007506:Iron-Sulfur Proteins; C426067:NFU1 protein, human; D011994:Recombinant Proteins; D029701:Saccharomyces cerevisiae Proteins; C080001:nifS protein, Bacteria; D013455:Sulfur; D007501:Iron; C545181:Grx3 protein, S cerevisiae; D010088:Oxidoreductases
keywords: IscU; Nfu; [2Fe-2S] cluster transfer; glutaredoxin; iron-sulfur cluster