title: Antimalarial Properties of Aqueous Crude Extracts ofGynostemma pentaphyllumandMoringa oleiferaLeaves in Combination with Artesunate inPlasmodium berghei-Infected Mice.
abstract: Due to the emergence and spread of malaria parasite with resistance to antimalarial drugs, discovery and development of new, safe, and affordable antimalarial are urgently needed. In this respect, medicinal plant extracts are targets to optimize antimalarial actions and restore efficacy of standard antimalarial drugs. The present study was aimed at determining the antimalarial activities ofGynostemma pentaphyllumandMoringa oleiferaleaf extracts in combination with artesunate againstPlasmodium berghei-infected mice.P. bergheiANKA maintained by serial passage in ICR mice were used based on intraperitoneal injection of 1 × 107parasitized erythrocytes and subsequent development of parasitemia. These infected mice were used to investigate the antimalarial activity of artesunate (6 mg/kg) in combination with 500, 1,000, and 2,000 mg/kg ofG. pentaphyllumandM. oleiferaleaf extracts using 4-day suppressive test. It was found that these extracts showed significant (P< 0.05) antimalarial activity in dose-dependent manner with percentage of suppression of 45, 50, and 55% forG. pentaphyllumleaf extract and 35, 40, and 50% forM. oleiferaleaf extract. Additionally, artesunate combined with these extracts presented higher antimalarial activity, compared to extract treated alone with percentage of suppression of 78, 91, and 96% forG. pentaphyllumleaf extract and 73, 82, and 91% forM. oleiferaleaf extract. The results indicated that combination treatment ofG. pentaphyllumorM. oleiferaleaf extracts with artesunate was able to increase the antimalarial activity by using low dose of artesunate. Hence, these results justified the combination of these extracts and artesunate in antimalarial herbal remedies.
authors.affiliation: Department of Clinical Chemistry, Faculty of Medical Technology, Western University, Kanchanaburi 71170, Thailand.
authors.forename: Voravuth
authors.identifier: 0000-0001-9774-2139
authors.initials: V
authors.lastname: Somsak
authors.affiliation: Department of Clinical Chemistry, Faculty of Medical Technology, Western University, Kanchanaburi 71170, Thailand.
authors.forename: Preeyanuch
authors.initials: P
authors.lastname: Borkaew
authors.affiliation: Department of Clinical Chemistry, Faculty of Medical Technology, Western University, Kanchanaburi 71170, Thailand.
authors.forename: Chokdee
authors.initials: C
authors.lastname: Klubsri
authors.affiliation: Department of Clinical Chemistry, Faculty of Medical Technology, Western University, Kanchanaburi 71170, Thailand.
authors.forename: Kittiyaporn
authors.initials: K
authors.lastname: Dondee
authors.affiliation: Department of Clinical Chemistry, Faculty of Medical Technology, Western University, Kanchanaburi 71170, Thailand.
authors.forename: Panatda
authors.initials: P
authors.lastname: Bootprom
authors.affiliation: Department of Clinical Chemistry, Faculty of Medical Technology, Western University, Kanchanaburi 71170, Thailand.
authors.forename: Butsarat
authors.initials: B
authors.lastname: Saiphet