title: Apoptotic neuron-secreted HN12 inhibits cell apoptosis in Hirschsprung's disease.
abstract: Perturbation in apoptosis can lead to Hirschsprung's disease (HSCR), which is a genetic disorder of neural crest development. It is believed that long noncoding RNAs (lncRNAs) play a role in the progression of HSCR. This study shows that apoptotic neurons can suppress apoptosis of nonapoptotic cells by secreting exosomes that contain high levels of HN12 lncRNA. Elevated exogenous HN12 in nonapoptotic cells effectively inhibited cell apoptosis by maintaining the function of mitochondria, including the production of ATP and the release of cytochrome C. These results demonstrate that secreted lncRNAs may serve as signaling molecules mediating intercellular communication in HSCR. In addition, high HN12 levels in the circulation worked as a biomarker for predicting HSCR, providing a potential, novel, noninvasive diagnostic approach for early screening of HSCR.
authors.affiliation: Department of Pediatric Surgery, Nanjing Children's Hospital Affiliated to Nanjing Medical University; State Key Laboratory of Reproductive Medicine, Institute of Toxicology, School of Public Health.
authors.forename: Chunxia
authors.initials: C
authors.lastname: Du
authors.affiliation: Department of Pediatric Surgery, Nanjing Children's Hospital Affiliated to Nanjing Medical University; State Key Laboratory of Reproductive Medicine, Institute of Toxicology, School of Public Health.
authors.forename: Hua
authors.initials: H
authors.lastname: Xie
authors.affiliation: Department of Pediatric Surgery, Nanjing Children's Hospital Affiliated to Nanjing Medical University; State Key Laboratory of Reproductive Medicine, Institute of Toxicology, School of Public Health.
authors.forename: Rujin
authors.initials: R
authors.lastname: Zang
authors.affiliation: Department of Pediatric Surgery, Nanjing Children's Hospital Affiliated to Nanjing Medical University; State Key Laboratory of Reproductive Medicine, Institute of Toxicology, School of Public Health.
authors.forename: Ziyang
authors.initials: Z
authors.lastname: Shen
authors.affiliation: Department of Pediatric Surgery, Nanjing Children's Hospital Affiliated to Nanjing Medical University; State Key Laboratory of Reproductive Medicine, Institute of Toxicology, School of Public Health.
authors.forename: Hongxing
authors.initials: H
authors.lastname: Li
authors.affiliation: Department of Pediatric Surgery, Nanjing Children's Hospital Affiliated to Nanjing Medical University; State Key Laboratory of Reproductive Medicine, Institute of Toxicology, School of Public Health.
authors.forename: Pingfa
authors.initials: P
authors.lastname: Chen
authors.affiliation: Department of Pediatric Surgery, Nanjing Children's Hospital Affiliated to Nanjing Medical University; State Key Laboratory of Reproductive Medicine, Institute of Toxicology, School of Public Health.
authors.forename: Xiaoqun
authors.initials: X
authors.lastname: Xu
authors.affiliation: State Key Laboratory of Reproductive Medicine, Institute of Toxicology, School of Public Health; Key Laboratory of Modern Toxicology, Ministry of Education, Nanjing Medical University, Nanjing, People's Republic of China.
authors.forename: Yankai
authors.initials: Y
authors.lastname: Xia
authors.affiliation: Department of Pediatric Surgery, Nanjing Children's Hospital Affiliated to Nanjing Medical University; State Key Laboratory of Reproductive Medicine, Institute of Toxicology, School of Public Health.
authors.forename: Weibing
authors.initials: W
authors.lastname: Tang
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D017209:Apoptosis / Q000235:genetics*; D015415:Biomarkers / Q000378:metabolism; D016022:Case-Control Studies; D002460:Cell Line; D003106:Colon / Q000473:pathology; D045304:Cytochromes c / Q000378:metabolism; D055354:Exosomes / Q000378:metabolism; D005260:Female; D005786:Gene Expression Regulation; D006627:Hirschsprung Disease / Q000175:diagnosis / Q000235:genetics* / Q000473:pathology; D006801:Humans; D007231:Infant, Newborn; D036341:Intercellular Signaling Peptides and Proteins; D008297:Male; D008928:Mitochondria / Q000235:genetics / Q000473:pathology; D009474:Neurons / Q000378:metabolism / Q000473:pathology*; D011506:Proteins / Q000737:chemistry / Q000235:genetics*; D062085:RNA, Long Noncoding / Q000235:genetics* / Q000378:metabolism
chemical_list: D015415:Biomarkers; D036341:Intercellular Signaling Peptides and Proteins; C000607374:MTRNR2L1 protein, human; D011506:Proteins; D062085:RNA, Long Noncoding; D000255:Adenosine Triphosphate; D045304:Cytochromes c
keywords: Hirschsprung’s disease; apoptosis; exosomal long noncoding RNA; intercellular communication; mitochondria; neuronal development