title: Evaluation of Hypoglycemic and Genotoxic Effect of Polyphenolic Bark Extract fromQuercus sideroxyla.
abstract: Quercus sideroxylais a wood species whose bark has phenolic compound and should be considered to be bioactive; the hypoglycemic and genotoxic properties ofQ.sideroxylabark were evaluated in this study. Total phenolic compound was determined in crude extract (CE) and organic extract (OE). The OE has the highest amount of phenols (724.1 ± 12.0 GAE/g). Besides, both CE and OE demonstrated effect over the inhibition ofα-amylasein vitro. Hypoglycemic activity was assessed by glucose tolerance curve and the area under curve (UAC); OE showed the highest hypoglycemic activity. In addition, diabetes was induced by streptozotocin (65 mg/kg) and the extracts (50 mg/kg) were administered for 10 days; OE showed hypoglycemic effect compared with diabetic control and decreased hepatic lipid peroxidation. Acute toxicity and genotoxicity were evaluated in CE; results of acute toxicity did not show any mortality. Besides, the comet assay showed that CE at a dose of 100 mg/kg did not show any genotoxic effect when evaluated at 24 h, whereas it induced slight damage at 200 mg/kg, with the formation of type 1 comets.
authors.affiliation: CIIDIR-Unidad Durango, Instituto Politécnico Nacional, Sigma 119 Fraccionamiento 20 de Noviembre, 34220 Durango, DGO, Mexico.
authors.forename: Marcela
authors.initials: M
authors.lastname: Soto-García
authors.affiliation: CIIDIR-Unidad Durango, Instituto Politécnico Nacional, Sigma 119 Fraccionamiento 20 de Noviembre, 34220 Durango, DGO, Mexico.
authors.forename: Martha
authors.initials: M
authors.lastname: Rosales-Castro
authors.affiliation: Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Wilfrido Massieu s/n, Esquina Manuel L. Stampa, Colonia Unidad Profesional Adolfo López Mateos, Delegación Gustavo A. Madero, 07738 Ciudad de México, Mexico.
authors.forename: Gerardo N
authors.initials: GN
authors.lastname: Escalona-Cardoso
authors.affiliation: Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Wilfrido Massieu s/n, Esquina Manuel L. Stampa, Colonia Unidad Profesional Adolfo López Mateos, Delegación Gustavo A. Madero, 07738 Ciudad de México, Mexico.
authors.forename: Norma
authors.identifier: 0000-0002-2434-4145
authors.initials: N
authors.lastname: Paniagua-Castro