title: Regenerative efficacy of mesenchymal stromal cells from human placenta in sensorineural hearing loss.
abstract: BACKGROUND
  Hearing loss is a common chronic disorder characterized by decline of auditory function. The global population have suffered from deafness and the transplantation of stem cells is regarded as a therapeutic strategy for this disease.
  
  
  METHODS
  We collected placenta from a total of 13 samples of full term pregnant women and isolated MSCs derived from human placenta and transplanted MSCs on deaf animal model. The normal group and the sensorineural hearing loss (SNHL) group and the experimental (transplanted MSCs) group were compared and estimated hearing level using auditory brainstem response (ABR) recordings and the otoacoustic emission (OAE) test.
  
  
  RESULTS
  ABR threshold value and DPOAE level showed that MSCs transplantation groups was improved than the SNHL group. And the number of spiral ganglion neurons were increased in all turn of the cochlea. And there was no evidence of acute immunological rejection and inflammation response was not observed.
  
  
  CONCLUSIONS
  This study is to evaluate regenerative efficacy of hearing loss by transplanting mesenchymal stromal cells (MSCs) derived from human placenta (amnion and chorion) in deaf animal model. We identified that MSCs transplantation restored auditory impairment and promoted cell regeneration. We hope to overcome sensorineural hearing loss by transplanting stem cells such as mesenchymal stromal cells (MSCs) from easily accessible adult stem cell source in placenta.
authors.affiliation: Department of Obstetrics and Gynecology, Seoul St. Mary's Hospital, The Catholic University of Korea School of Medicine, Seoul, South Korea.
authors.forename: Kicheol
authors.initials: K
authors.lastname: Kil
authors.affiliation: Department of Medical Cell Biology, The Catholic University of Korea School of Medicine, Seoul, South Korea; Department of Otolaryngology-Head & Neck Surgery, Seoul St. Mary's Hospital, The Catholic University of Korea School of Medicine, Seoul, South Korea.
authors.forename: Mi Young
authors.initials: MY
authors.lastname: Choi
authors.affiliation: Department of Otolaryngology-Head & Neck Surgery, Seoul St. Mary's Hospital, The Catholic University of Korea School of Medicine, Seoul, South Korea.
authors.forename: Ji Sun
authors.initials: JS
authors.lastname: Kong
authors.affiliation: Department of Otolaryngology-Head & Neck Surgery, Seoul St. Mary's Hospital, The Catholic University of Korea School of Medicine, Seoul, South Korea.
authors.forename: Woo Jin
authors.initials: WJ
authors.lastname: Kim
authors.affiliation: Department of Otolaryngology-Head & Neck Surgery, Seoul St. Mary's Hospital, The Catholic University of Korea School of Medicine, Seoul, South Korea. Electronic address: khpent@catholic.ac.kr.
authors.forename: Kyoung Ho
authors.initials: KH
authors.lastname: Park
mesh_terms: D000818:Animals; D001309:Auditory Threshold; D004195:Disease Models, Animal; D016057:Evoked Potentials, Auditory, Brain Stem; D005260:Female; D006309:Hearing; D006319:Hearing Loss, Sensorineural / Q000473:pathology / Q000503:physiopathology* / Q000628:therapy*; D006801:Humans; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000502:physiology*; D010920:Placenta / Q000166:cytology; D011247:Pregnancy; D012038:Regeneration; D013136:Spiral Ganglion / Q000473:pathology / Q000502:physiology*
keywords: Amnion; Chorion; Hearing loss; Mesenchymal stromal cell