title: Aqueous Extract ofClerodendranthus spicatusExerts Protective Effect on UV-Induced Photoaged Mice Skin.
abstract: Clerodendranthus spicatus(Thunb.) C.Y.Wu (CS) is commonly used to treat kidney diseases in traditional Chinese medicine for its prominent anti-inflammatory effect and nourishing function to kidneys. In this study, aqueous extract of CS was assessed for its protective effect on UV-induced skin damage of mice. The chemical compositions of CS aqueous extract were determined by HPLC-ESI-MS/MS, in which 10 components were identified. During the experimental period, CS (0.9, 1.8, and 3.6 g/mL) was externally applied to shaved dorsal skins of mice prior to UV irradiation, daily for ten weeks. The results presented that CS (3.6 g/mL) apparently improved photodamaged skin appearance such as erythema, edema, and coarseness. The abnormal epidermal thickening was significantly reduced, and the dermal structures became more complete. The underlying protective mechanisms were associated with improving antioxidant enzymes activities including superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px), downregulating inflammatory cytokines (IL-1β, IL-6, TNF-α, COX-2, and PGE2) expressions, recovering collagen density, and reducing matrix metalloproteinases productions. Sun protection factor of CS (3.6 g/mL) was 16.21 ± 0.03. Our findings for the first time demonstrated that CS had therapeutic effect on the photoaged skin. The results indicated that CS is a potential agent for photoprotective cosmetics.
authors.affiliation: The First Affiliated Hospital of Chinese Medicine, Guangzhou University of Chinese Medicine, 12 Airport Road, Bai Yun District, Guangzhou 510405, China.
authors.forename: Lan
authors.identifier: 0000-0001-7701-2219
authors.initials: L
authors.lastname: Wang
authors.affiliation: School of Chinese Medicine, Faculty of Science, The Chinese University of Hong Kong, Shatin, Hong Kong.
authors.forename: Xie
authors.identifier: 0000-0002-3113-9633
authors.initials: X
authors.lastname: Zhang
authors.affiliation: The First Affiliated Hospital of Chinese Medicine, Guangzhou University of Chinese Medicine, 12 Airport Road, Bai Yun District, Guangzhou 510405, China.
authors.forename: Yong-Xian
authors.identifier: 0000-0002-4897-1194
authors.initials: YX
authors.lastname: Li
authors.affiliation: School of Chinese Materia Medica, Guangzhou University of Chinese Medicine, Guangzhou Higher Education Mega Center, 232 Wai Huan Dong Road, Guangzhou 510006, China.
authors.forename: Lie-Qiang
authors.identifier: 0000-0001-6094-9873
authors.initials: LQ
authors.lastname: Xu
authors.affiliation: School of Chinese Materia Medica, Guangzhou University of Chinese Medicine, Guangzhou Higher Education Mega Center, 232 Wai Huan Dong Road, Guangzhou 510006, China.
authors.forename: Cai-Lan
authors.initials: CL
authors.lastname: Li
authors.affiliation: School of Chinese Materia Medica, Guangzhou University of Chinese Medicine, Guangzhou Higher Education Mega Center, 232 Wai Huan Dong Road, Guangzhou 510006, China.
authors.forename: Zhen-Biao
authors.identifier: 0000-0002-9556-8103
authors.initials: ZB
authors.lastname: Zhang
authors.affiliation: School of Chinese Materia Medica, Guangzhou University of Chinese Medicine, Guangzhou Higher Education Mega Center, 232 Wai Huan Dong Road, Guangzhou 510006, China.
authors.forename: Jia-Li
authors.identifier: 0000-0002-2800-7349
authors.initials: JL
authors.lastname: Liang
authors.affiliation: School of Chinese Materia Medica, Guangzhou University of Chinese Medicine, Guangzhou Higher Education Mega Center, 232 Wai Huan Dong Road, Guangzhou 510006, China.
authors.forename: Zi-Ren
authors.identifier: 0000-0002-7018-3207
authors.initials: ZR
authors.lastname: Su
authors.affiliation: The First Affiliated Hospital of Chinese Medicine, Guangzhou University of Chinese Medicine, 12 Airport Road, Bai Yun District, Guangzhou 510405, China.
authors.forename: Hui-Fang
authors.identifier: 0000-0002-4841-9084
authors.initials: HF
authors.lastname: Zeng
authors.affiliation: School of Chinese Materia Medica, Guangzhou University of Chinese Medicine, Guangzhou Higher Education Mega Center, 232 Wai Huan Dong Road, Guangzhou 510006, China.
authors.forename: Yu-Cui
authors.identifier: 0000-0002-0917-6198
authors.initials: YC
authors.lastname: Li