title: Microsatellite primers for the rare sedgeLepidosperma bungalbin(Cyperaceae).
abstract: OBJECTIVE
  Microsatellite markers were developed for the rare sedgeLepidosperma bungalbin(Cyperaceae) to assess genetic variation and its spatial structuring.
  
  
  RESULTS
  We conducted shotgun sequencing on an Illumina MiSeq and produced 6,215,872 sequence reads. The QDD pipeline was used to design 60 primer pairs that were screened using PCR. We developed 17 loci, of which 12 loci were identified that were polymorphic, amplified reliably, and could be consistently scored. We then screened these loci for variation in individuals from three populations. The number of alleles observed for these 12 loci across the three populations ranged from nine to 19 and expected heterozygosity ranged from 0.41 to 0.89.
  
  
  CONCLUSIONS
  These markers will enable the quantification of the potential impact of mining on genetic variation withinL. bungalbinand establish a baseline for future management of genetic variation of the rare sedge.
authors.affiliation: Australian Research Council Centre for Mine Restoration, Department of Environment and Agriculture, Curtin University, Bentley 6102, Western Australia, Australia; School of Plant Biology, University of Western Australia, Nedlands 6009, Western Australia, Australia.
authors.forename: Paul G
authors.initials: PG
authors.lastname: Nevill
authors.affiliation: Australian Research Council Centre for Mine Restoration, Department of Environment and Agriculture, Curtin University, Bentley 6102, Western Australia, Australia.
authors.forename: Grant
authors.initials: G
authors.lastname: Wardell-Johnson
keywords: Cyperaceae; Lepidosperma bungalbin; South West Australian Floristic Region (SWAFR); microsatellite primers; shotgun sequencing