title: Low-voltage scanning electron microscopy study of lampbrush chromosomes and nuclear bodies in avian and amphibian oocytes.
abstract: Nucleus is a highly compartmentalized part of the cell where the key processes of genome functionality are realized through the formation of non-membranous nuclear domains. Physically nuclear domains appear as liquid droplets with different viscosity stably maintained throughout the interphase or during the long diplotene stage of meiosis. Since nuclear body surface represents boundary between two liquid phases, the ultrastructural surface topography of nuclear domains is of an outstanding interest. The aim of this study was to examine ultrathin surface topography of the amphibian and avian oocyte nuclear structures such as lampbrush chromosomes, nucleoli, histone-locus bodies, Cajal body-like bodies, and the interchromatin granule clusters via low-voltage scanning electron microscopy. Our results demonstrate that nuclear bodies with similar molecular composition may differ dramatically in the surface topography and vice versa, nuclear bodies that do not share common molecular components may possess similar topographical characteristics. We also have analyzed surface distribution of particular nuclear antigens (double stranded DNA, coilin and splicing snRNA) using indirect immunogold labeling with subsequent secondary electron detection of gold nanoparticles. We suggest that ultrastructural surface morphology reflects functional status of a nuclear body.
authors.affiliation: Saint-Petersburg State University, Saint-Petersburg, Russia.
authors.forename: Tatiana
authors.initials: T
authors.lastname: Kulikova
authors.affiliation: Saint-Petersburg State University, Saint-Petersburg, Russia.
authors.forename: Tatiana
authors.initials: T
authors.lastname: Khodyuchenko
authors.affiliation: Saint-Petersburg State University, Saint-Petersburg, Russia.
authors.forename: Yuri
authors.initials: Y
authors.lastname: Petrov
authors.affiliation: Saint-Petersburg State University, Saint-Petersburg, Russia.
authors.forename: Alla
authors.initials: A
authors.lastname: Krasikova
mesh_terms: D000663:Amphibians; D000818:Animals; D001717:Birds; D002467:Cell Nucleus / Q000648:ultrastructure*; D002875:Chromosomes / Q000648:ultrastructure*; D020541:Coiled Bodies / Q000648:ultrastructure*; D008855:Microscopy, Electron, Scanning / Q000379:methods*; D009865:Oocytes / Q000648:ultrastructure*; D013499:Surface Properties