title: Membrane-bound glycerol dehydrogenase catalyzes oxidation of D-pentonates to 4-keto-D-pentonates, D-fructose to 5-keto-D-fructose, and D-psicose to 5-keto-D-psicose.
abstract: A novel oxidation of D-pentonates to 4-keto-D-pentonates was analyzed with Gluconobacter thailandicus NBRC 3258. D-Pentonate 4-dehydrogenase activity in the membrane fraction was readily inactivated by EDTA and it was reactivated by the addition of PQQ and Ca2+. D-Pentonate 4-dehydrogenase was purified to two different subunits, 80 and 14 kDa. The absorption spectrum of the purified enzyme showed no typical absorbance over the visible regions. The enzyme oxidized D-pentonates to 4-keto-D-pentonates at the optimum pH of 4.0. In addition, the enzyme oxidized D-fructose to 5-keto-D-fructose, D-psicose to 5-keto-D-psicose, including the other polyols such as, glycerol, D-ribitol, D-arabitol, and D-sorbitol. Thus, D-pentonate 4-dehydrogenase was found to be identical with glycerol dehydrogenase (GLDH), a major polyol dehydrogenase in Gluconobacter species. The reaction versatility of quinoprotein GLDH was notified in this study.
authors.affiliation: a Department of Bioscience , Graduate School of Agriculture, Ehime University , Matsuyama , Japan.
authors.forename: Yoshitaka
authors.initials: Y
authors.lastname: Ano
authors.affiliation: b CINDEFI , School of Science, La Plata National University , La Plata , Argentina.
authors.forename: Roque A
authors.initials: RA
authors.lastname: Hours
authors.affiliation: c Faculty of Agriculture, Department of Biological Chemistry , Yamaguchi University , Yamaguchi , Japan.
authors.forename: Yoshihiko
authors.initials: Y
authors.lastname: Akakabe
authors.affiliation: c Faculty of Agriculture, Department of Biological Chemistry , Yamaguchi University , Yamaguchi , Japan.
authors.forename: Naoya
authors.initials: N
authors.lastname: Kataoka
authors.affiliation: c Faculty of Agriculture, Department of Biological Chemistry , Yamaguchi University , Yamaguchi , Japan.
authors.forename: Toshiharu
authors.initials: T
authors.lastname: Yakushi
authors.affiliation: c Faculty of Agriculture, Department of Biological Chemistry , Yamaguchi University , Yamaguchi , Japan.
authors.forename: Kazunobu
authors.initials: K
authors.lastname: Matsushita
authors.affiliation: c Faculty of Agriculture, Department of Biological Chemistry , Yamaguchi University , Yamaguchi , Japan.
authors.forename: Osao
authors.initials: O
authors.lastname: Adachi
mesh_terms: D055162:Biocatalysis; D002462:Cell Membrane / Q000201:enzymology* / Q000378:metabolism; D005632:Fructose / Q000031:analogs & derivatives* / Q000737:chemistry; D023281:Genomics; D020576:Gluconobacter / Q000201:enzymology; D010084:Oxidation-Reduction; D012995:Solubility; D013401:Sugar Alcohol Dehydrogenases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*
chemical_list: C033856:5-ketofructose; C003243:psicose; D005632:Fructose; D013401:Sugar Alcohol Dehydrogenases; C020672:glycerol dehydrogenase
keywords: 4-keto-D-arabonate production; 4-keto-D-pentonate; acetic acid bacteria; glycerol dehydrogenase; oxidative fermentation