title: An imprinted long noncoding RNA located between genes Meg8 and Meg9 in the cattle Dlk1-Dio3 domain.
abstract: The Dlk1-Dio3 imprinted domain is located on the cattle chromosome 21 and contains three paternally expressed protein-coding genes and a number of maternally expressed short or long noncoding RNA genes. We have previously obtained two maternally expressed long noncoding RNA genes, Meg8 and Meg9, from the cattle. In this study, we identified a novel noncoding RNA located between Meg8 and Meg9 known as LINC24061 according to the GENCODE annotated bibliography. Two alternatively spliced transcripts (LINC24061-v1 and LINC24061-v2) were obtained using RT-PCR and RACE, and the expression pattern of LINC24061-v1 and LINC24061-v2 was shown to be tissue-specific. The LINC24061-v1 splice variant was expressed in only three types of tissues: heart, kidney and muscle; in contrast, LINC24061-v2 was expressed in all eight tissues examined, including heart, liver, spleen, lung, kidney, skeletal muscle, subcutaneous fat, and brain of adult cattle. The allele-specific expression of LINC24061 was identified based on a single nucleotide polymorphism (SNP) in exon 2 of LINC24061. The results showed that LINC24061 exhibited monoallelic expression in all the examined cattle tissues.
authors.affiliation: Department of Biochemistry and Molecular Biology, College of Life Science, Agriculture University of Hebei, Baoding, China.
authors.forename: Mingyue
authors.initials: M
authors.lastname: Zhang
authors.affiliation: Hebei North University, Zhangjiakou, China.
authors.forename: Yupeng
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: Department of Biochemistry and Molecular Biology, College of Life Science, Agriculture University of Hebei, Baoding, China.
authors.forename: Guannan
authors.initials: G
authors.lastname: Wang
authors.affiliation: College of Life Science and Life Engineering, Hebei University of Science and Technology, Shijiazhuang, China.
authors.forename: Dongjie
authors.initials: D
authors.lastname: Li
authors.affiliation: College of Medical Science, Hebei University, Baoding, China.
authors.forename: Weina
authors.initials: W
authors.lastname: Chen
authors.affiliation: Department of Biochemistry and Molecular Biology, College of Life Science, Agriculture University of Hebei, Baoding, China.
authors.forename: Cui
authors.initials: C
authors.lastname: Zhang
authors.affiliation: Department of Biochemistry and Molecular Biology, College of Life Science, Agriculture University of Hebei, Baoding, China. lishijie20005@163.com.
authors.forename: Shijie
authors.initials: S
authors.lastname: Li
mesh_terms: D000483:Alleles; D017398:Alternative Splicing; D000818:Animals; D002417:Cattle; D002875:Chromosomes; D003001:Cloning, Molecular; D015870:Gene Expression; D020869:Gene Expression Profiling; D023061:Gene Order; D056426:Genetic Loci; D018392:Genomic Imprinting; D005838:Genotype; D020641:Polymorphism, Single Nucleotide; D062085:RNA, Long Noncoding
chemical_list: D062085:RNA, Long Noncoding
keywords: Allele expression; Cows; LINC24061; Splice variants; lncRNA