title: In silicoIdentification of Potential Peptides or Allergen Shot Candidates AgainstAspergillus fumigatus.
abstract: Aspergillus fumigatusis capable of causing invasive aspergillosis or acute bronchopulmonary aspergillosis, and the current situation is alarming. There are no vaccine or allergen shots available forAspergillus-induced allergies. Thus, a novel approach in designing of an effective vaccine or allergen shot candidate againstA. fumigatusis needed. Using immunoinformatics approaches from the characterizedA. fumigatusallergens, we have mapped epitopic regions to predict potential peptides that elicit bothAspergillus-specific T cells and B cell immune response. Experimentally derived immunodominant allergens were retrieved from www.allergen.org. A total of 23 allergenic proteins ofA. fumigatuswere retrieved. Out of 23 allergenic proteins, 13 of them showed high sequence similarity to both human and mouse counterparts and thus were eliminated from analysis due to possible cross-reactivity. Remaining allergens were subjected to T cell (major histocompatibility complex class I and II alleles) and B cell epitope prediction using immune epitope database analysis resource. Only five allergens have shown a common B and T cell epitopic region between human and mouse. They areAsp f1{147-156 region (RVIYTYPNKV); Mitogillin},Asp f2{5-19 region (LRLAVLLPLAAPLVA); Hypothetical protein},Asp f5{305-322 region (LNNYRPSSSSLSFKY); Metalloprotease},Asp f17{98-106 region (AANAGGTVY); Hypothetical protein}, andAsp f34{74-82 region (YIQDGSLYL); PhiA cell wall protein}. The epitopic region from these five allergenic proteins showed potential for development of single peptide- or multipeptide-based vaccine or allergen shots for experimental prioritization.
authors.affiliation: Department of Biotechnology and Bioinformatics, Jaypee University of Information Technology , Solan-173234 (Himachal Pradesh), India .
authors.forename: Raman
authors.initials: R
authors.lastname: Thakur
authors.affiliation: Department of Biotechnology and Bioinformatics, Jaypee University of Information Technology , Solan-173234 (Himachal Pradesh), India .
authors.forename: Jata
authors.initials: J
authors.lastname: Shankar
keywords: Asp f34; Aspergillus fumigatus; allergens; epitopes; vaccine; vaccine design