title: In VitroTNF-αInhibitory Activity of Brazilian Plants and Anti-Inflammatory Effect ofStryphnodendron adstringensin an Acute Arthritis Model.
abstract: Stryphnodendronspecies, popularly named "barbatimão," are traditionally used in Brazil as anti-inflammatory agents. This study aimed to investigate the effect of barbatimão and 11 other species on the production of tumor necrosis factor-alpha (TNF-α) in lipopolysaccharide- (LPS-) stimulated THP-1 cells, as well as their anti-arthritis activity. The extracts ofStryphnodendron adstringens,Stryphnodendron obovatum,Campomanesia lineatifolia, andTerminalia glabrescenspromoted a concentration-dependent inhibition of TNF-α. Mice injected with LPS in the knee joint were treatedper oswith fractions from the selected extracts. Both the organic (SAO) and the aqueous (SAA) fractions ofS. adstringenspromoted a dose-dependent reduction of leukocyte migration and neutrophil accumulation into the joint, but none of them reduced CXCL1 concentration in the periarticular tissue. In contrast, treatment withC. lineatifoliaandT. glabrescensfractions did not ameliorate the inflammatory parameters. Analyses of SAO by Ultra Performance Liquid Chromatography (UPLC) coupled to electrospray ionization mass spectrometry (ESI-MS) led to the identification of gallic acid along with 11 prodelphinidins, characterized as monomers and dimers of the B-type. Our findings contribute to some extent to corroborating the traditional use ofS. adstringensas an anti-inflammatory agent. This activity is probably related to a decrease of leukocyte migration into the inflammatory site. Polyphenols like gallic acid and prodelphinidins, identified in the active fraction, may contribute to the observed activity.
authors.affiliation: Department of Pharmaceutical Sciences, Faculty of Pharmacy, Universidade Federal de Minas Gerais, Campus Pampulha, Avenida Antônio Carlos 6627, 31.270-901 Belo Horizonte, MG, Brazil.
authors.forename: Bárbara O
authors.initials: BO
authors.lastname: Henriques
authors.affiliation: Department of Pharmaceutical Sciences, Faculty of Pharmacy, Universidade Federal de Minas Gerais, Campus Pampulha, Avenida Antônio Carlos 6627, 31.270-901 Belo Horizonte, MG, Brazil.
authors.forename: Olívia
authors.initials: O
authors.lastname: Corrêa
authors.affiliation: Department of Pharmaceutical Sciences, Faculty of Pharmacy, Universidade Federal de Minas Gerais, Campus Pampulha, Avenida Antônio Carlos 6627, 31.270-901 Belo Horizonte, MG, Brazil.
authors.forename: Elaine Patrícia C
authors.initials: EP
authors.lastname: Azevedo
authors.affiliation: Department of Pharmaceutical Sciences, Faculty of Pharmacy, Universidade Federal de Minas Gerais, Campus Pampulha, Avenida Antônio Carlos 6627, 31.270-901 Belo Horizonte, MG, Brazil.
authors.forename: Rodrigo M
authors.initials: RM
authors.lastname: Pádua
authors.affiliation: Department of Biochemistry and Immunology, Institute of Biological Sciences, Universidade Federal de Minas Gerais, Campus Pampulha, Avenida Antônio Carlos 6627, 31.270-901 Belo Horizonte, MG, Brazil.
authors.forename: Vívian Louise S
authors.initials: VL
authors.lastname: de Oliveira
authors.affiliation: Department of Biochemistry and Immunology, Institute of Biological Sciences, Universidade Federal de Minas Gerais, Campus Pampulha, Avenida Antônio Carlos 6627, 31.270-901 Belo Horizonte, MG, Brazil.
authors.forename: Thiago Henrique C
authors.initials: TH
authors.lastname: Oliveira
authors.affiliation: Department of Biochemistry and Immunology, Institute of Biological Sciences, Universidade Federal de Minas Gerais, Campus Pampulha, Avenida Antônio Carlos 6627, 31.270-901 Belo Horizonte, MG, Brazil.
authors.forename: Daiane
authors.initials: D
authors.lastname: Boff
authors.affiliation: Department of Microbiology, Institute of Biological Sciences, Universidade Federal de Minas Gerais, Campus Pampulha, Avenida Antônio Carlos 6627, 31.270-901 Belo Horizonte, MG, Brazil.
authors.forename: Ana Carolina F
authors.initials: AC
authors.lastname: Dias
authors.affiliation: Department of Microbiology, Institute of Biological Sciences, Universidade Federal de Minas Gerais, Campus Pampulha, Avenida Antônio Carlos 6627, 31.270-901 Belo Horizonte, MG, Brazil.
authors.forename: Danielle G
authors.identifier: 0000-0002-7478-5934
authors.initials: DG
authors.lastname: de Souza
authors.affiliation: Department of Biochemistry and Immunology, Institute of Biological Sciences, Universidade Federal de Minas Gerais, Campus Pampulha, Avenida Antônio Carlos 6627, 31.270-901 Belo Horizonte, MG, Brazil.
authors.forename: Flávio A
authors.initials: FA
authors.lastname: Amaral
authors.affiliation: Department of Biochemistry and Immunology, Institute of Biological Sciences, Universidade Federal de Minas Gerais, Campus Pampulha, Avenida Antônio Carlos 6627, 31.270-901 Belo Horizonte, MG, Brazil.
authors.forename: Mauro M
authors.identifier: 0000-0002-6944-3008
authors.initials: MM
authors.lastname: Teixeira
authors.affiliation: Department of Pharmaceutical Sciences, Faculty of Pharmacy, Universidade Federal de Minas Gerais, Campus Pampulha, Avenida Antônio Carlos 6627, 31.270-901 Belo Horizonte, MG, Brazil.
authors.forename: Rachel O
authors.initials: RO
authors.lastname: Castilho
authors.affiliation: Department of Pharmaceutical Sciences, Faculty of Pharmacy, Universidade Federal de Minas Gerais, Campus Pampulha, Avenida Antônio Carlos 6627, 31.270-901 Belo Horizonte, MG, Brazil.
authors.forename: Fernão C
authors.identifier: 0000-0001-9468-376X
authors.initials: FC
authors.lastname: Braga