title: Characterization of the LM5 pectic galactan epitope with synthetic analogues of β-1,4-d-galactotetraose.
abstract: Plant cell wall glycans are important polymers that are crucial to plant development and serve as an important source of sustainable biomass. The study of polysaccharides in the plant cell wall relies heavily on monoclonal antibodies (mAbs) for localization and visualization of glycans, using e.g. immunofluorescent microscopy. Here, we describe the detailed epitope mapping of the mAb LM5 that is shown to bind to a minimum of three sugar residues at the non-reducing end of linear beta-1,4-linked galactan. The study uses de novo synthetic analogues of galactans combined with carbohydrate microarray and competitive inhibition ELISA for analysis of antibody-carbohydrate interactions.
authors.affiliation: Center for Nanomedicine and Theranostics, Department of Chemistry, Technical University of Denmark, Kemitorvet, Building 207, Lyngby, Denmark.
authors.forename: Mathias C F
authors.initials: MC
authors.lastname: Andersen
authors.affiliation: Center for Nanomedicine and Theranostics, Department of Chemistry, Technical University of Denmark, Kemitorvet, Building 207, Lyngby, Denmark.
authors.forename: Irene
authors.initials: I
authors.lastname: Boos
authors.affiliation: Centre for Plant Sciences, Faculty of Biological Sciences, University of Leeds, Leeds LS2 9JT, United Kingdom.
authors.forename: Susan E
authors.initials: SE
authors.lastname: Marcus
authors.affiliation: Department of Plant and Environmental Sciences, Thorvaldsensvej 40, Frederiksberg C, Denmark.
authors.forename: Stjepan K
authors.initials: SK
authors.lastname: Kračun
authors.affiliation: Department of Plant and Environmental Sciences, Thorvaldsensvej 40, Frederiksberg C, Denmark.
authors.forename: Maja Gro
authors.initials: MG
authors.lastname: Rydahl
authors.affiliation: Department of Plant and Environmental Sciences, Thorvaldsensvej 40, Frederiksberg C, Denmark; School of Agriculture, Food and Rural Development, Newcastle University, Newcastle upon Tyne, United Kingdom.
authors.forename: William G T
authors.initials: WG
authors.lastname: Willats
authors.affiliation: Centre for Plant Sciences, Faculty of Biological Sciences, University of Leeds, Leeds LS2 9JT, United Kingdom.
authors.forename: J Paul
authors.initials: JP
authors.lastname: Knox
authors.affiliation: Center for Nanomedicine and Theranostics, Department of Chemistry, Technical University of Denmark, Kemitorvet, Building 207, Lyngby, Denmark. Electronic address: mhc@kemi.dtu.dk.
authors.forename: Mads H
authors.initials: MH
authors.lastname: Clausen
mesh_terms: D000911:Antibodies, Monoclonal / Q000737:chemistry / Q000378:metabolism*; D002473:Cell Wall / Q000737:chemistry*; D000939:Epitopes / Q000737:chemistry*; D005685:Galactans / Q000737:chemistry*; D005690:Galactose / Q000737:chemistry*; D009844:Oligosaccharides / Q000737:chemistry / Q000378:metabolism*; D010368:Pectins / Q000737:chemistry*
chemical_list: D000911:Antibodies, Monoclonal; D000939:Epitopes; D005685:Galactans; D009844:Oligosaccharides; D010368:Pectins; D005690:Galactose