title: Detection of trans-fatty acids by high performance liquid chromatography coupled with in-tube solid-phase microextraction using hydrophobic polymeric monolith.
abstract: Based on in-tube solid-phase microextraction (in-tube SPME) using a hydrophobic poly (octadecyl methacrylate-co-ethylene dimethacrylate) [poly (OMA-co-EDMA)] monolith, a simple high performance liquid chromatography (HPLC) method has been developed for detection of trans-fatty acids (TFAs) as their fatty acid methyl esters (FAMEs). The poly (OMA-co-EDMA) monolithic column with high hydrophobicity was specially prepared for simultaneous microextraction, pre-separation and purification for the analytes. The pre-separation selectivity, the extraction efficiency, and the purification effect for FAMEs were investigated respectively. Furthermore, some operation parameters have been optimized in detail with respect to satisfactory extraction efficiency of the target compounds. Under the optimized conditions, the enrichment factors for model FAMEs were ranged from 58.3 to 70.9, wide linear range (0.01-1.00mg/kg) and low detection limits (LODs) (3.0-7.1μg/kg) were achieved, respectively. In addition, recoveries of the method were in the range from 83.0 to 106.4% with low relative standard deviations (RSDs) of 3.2-4.7% (n=4) at spiking levels of 0.05, 0.25 and 0.5mg/kg, respectively. Finally, the proposed method was successfully applied for detection of TFAs in practical samples, which possesses short time-consuming, low disturbance and high detection sensitivity.
authors.affiliation: Institute of Biomedical and Pharmaceutical Technology, Fuzhou University, Fuzhou, 350002, China; Institute of Laser Chemistry, Department of Chemistry, Fudan University, Shanghai, 200433, China.
authors.forename: Fangling
authors.initials: F
authors.lastname: Wu
authors.affiliation: Institute of Biomedical and Pharmaceutical Technology, Fuzhou University, Fuzhou, 350002, China. Electronic address: jbwang@fzu.edu.cn.
authors.forename: Jiabin
authors.initials: J
authors.lastname: Wang
authors.affiliation: Institute of Biomedical and Pharmaceutical Technology, Fuzhou University, Fuzhou, 350002, China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Zhao
authors.affiliation: Institute of Biomedical and Pharmaceutical Technology, Fuzhou University, Fuzhou, 350002, China.
authors.forename: Nan
authors.initials: N
authors.lastname: Jiang
authors.affiliation: Institute of Food Safety and Environment Monitoring, Fuzhou University, Fuzhou, 350108, China.
authors.forename: Xucong
authors.initials: X
authors.lastname: Lin
authors.affiliation: Institute of Food Safety and Environment Monitoring, Fuzhou University, Fuzhou, 350108, China.
authors.forename: Zenghong
authors.initials: Z
authors.lastname: Xie
authors.affiliation: Institute of Biomedical and Pharmaceutical Technology, Fuzhou University, Fuzhou, 350002, China.
authors.forename: Jianhua
authors.initials: J
authors.lastname: Li
authors.affiliation: Institute of Biomedical and Pharmaceutical Technology, Fuzhou University, Fuzhou, 350002, China. Electronic address: zhangqiq@126.com.
authors.forename: Qiqing
authors.initials: Q
authors.lastname: Zhang
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003069:Coffee / Q000737:chemistry*; D004951:Esterification; D004952:Esters / Q000032:analysis; D057927:Hydrophobic and Hydrophilic Interactions; D057230:Limit of Detection; D008689:Methacrylates / Q000737:chemistry*; D008745:Methylation; D052617:Solid Phase Microextraction / Q000379:methods*; D044242:Trans Fatty Acids / Q000032:analysis*
chemical_list: D003069:Coffee; D004952:Esters; D008689:Methacrylates; D044242:Trans Fatty Acids; C004919:ethylene dimethacrylate
keywords: HPLC; In-tube solid-phase microextraction; Poly (OMA-co-EDMA) monolithic column; Trans-fatty acids