title: Magnetic mesoporous nanospheres anchored with LyP-1 as an efficient pancreatic cancer probe.
abstract: Immobilization of a ligand that selectively interacts with cancer cells to nanomaterials can enhance their diagnostic and therapeutic efficiency. In this study, we firstly demonstrate the high expression of receptor for cyclic nine-amino acid peptide LyP-1 (Cys-Gly-Asn-Lys-Arg-Thr-Arg-Gly-Cys) in both mouse and human pancreatic cancer. Based on these findings, sub-50 nm multifunctional superparamagnetic mesoporous nanospheres with surface modified with LyP-1 are rationally designed. Theses nanospheres have a core of silica-protected magnetite nanoparticle and a shell of FITC-labeled mesoporous silica, and they are able to specifically recognize and conjugate with the pancreatic cancer cell in vitro, as verified by the combined techniques of fluorescent imaging and T2 weight magnetic resonance imaging. After systematic administration, these LyP-1 immobilized nanospheres are found to actively target to mouse orthotopic xenograft of pancreatic cancer, which opens up the door for applications in early probing and diagnosis of pancreatic cancer by the multimodal imaging.
authors.affiliation: Department of Pancreatic Surgery, Nephrology & Radiology, Huashan Hospital, Fudan University, Shanghai 200040, China.
authors.forename: Yongjian
authors.initials: Y
authors.lastname: Jiang
authors.affiliation: Department of Pancreatic Surgery, Nephrology & Radiology, Huashan Hospital, Fudan University, Shanghai 200040, China.
authors.forename: Shaojun
authors.initials: S
authors.lastname: Liu
authors.affiliation: Department of Chemistry, State Key Laboratory of Molecular Engineering of Polymers, Shanghai Key Laboratory of Molecular Catalysis and Innovative Materials, Collaborative Innovation Center of Chemistry for Energy Materials, Fudan University, Shanghai 200433, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Pancreatic Surgery, Nephrology & Radiology, Huashan Hospital, Fudan University, Shanghai 200040, China.
authors.forename: Hengchao
authors.initials: H
authors.lastname: Li
authors.affiliation: Department of Pancreatic Surgery, Nephrology & Radiology, Huashan Hospital, Fudan University, Shanghai 200040, China.
authors.forename: Hang
authors.initials: H
authors.lastname: He
authors.affiliation: Department of Pancreatic Surgery, Nephrology & Radiology, Huashan Hospital, Fudan University, Shanghai 200040, China.
authors.forename: Juntao
authors.initials: J
authors.lastname: Dai
authors.affiliation: Department of Radiology, Changhai Hospital, Second Military Medical University, Shanghai 200433, China. Electronic address: laijiangtaotao@163.com.
authors.forename: Tao
authors.initials: T
authors.lastname: Jiang
authors.affiliation: Institute for Complex Engineered Systems, Carnegie Mellon University, Pittsburgh, PA 15213, USA.
authors.forename: Weihang
authors.initials: W
authors.lastname: Ji
authors.affiliation: Department of Pancreatic Surgery, Nephrology & Radiology, Huashan Hospital, Fudan University, Shanghai 200040, China.
authors.forename: Daoying
authors.initials: D
authors.lastname: Geng
authors.affiliation: Materials Science and Technology Program, College of Arts and Sciences, Qatar University, Doha 2713, Qatar.
authors.forename: Ahmed A
authors.initials: AA
authors.lastname: Elzatahry
authors.affiliation: Department of Chemistry, College of Science, King Saud University, Riyadh 11451, Saudi Arabia.
authors.forename: Abdulaziz
authors.initials: A
authors.lastname: Alghamdi
authors.affiliation: Department of Pancreatic Surgery, Nephrology & Radiology, Huashan Hospital, Fudan University, Shanghai 200040, China. Electronic address: surgeonfu@163.com.
authors.forename: Deliang
authors.initials: D
authors.lastname: Fu
authors.affiliation: Department of Chemistry, State Key Laboratory of Molecular Engineering of Polymers, Shanghai Key Laboratory of Molecular Catalysis and Innovative Materials, Collaborative Innovation Center of Chemistry for Energy Materials, Fudan University, Shanghai 200433, China; State Key Lab of Transducer Technology, Shanghai Institute of Microsystem and Information Technology, Chinese Academy of Sciences, Shanghai 200050, China. Electronic address: yhdeng@fudan.edu.cn.
authors.forename: Yonghui
authors.initials: Y
authors.lastname: Deng
authors.affiliation: Department of Chemistry, State Key Laboratory of Molecular Engineering of Polymers, Shanghai Key Laboratory of Molecular Catalysis and Innovative Materials, Collaborative Innovation Center of Chemistry for Energy Materials, Fudan University, Shanghai 200433, China.
authors.forename: Dongyuan
authors.initials: D
authors.lastname: Zhao
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D003287:Contrast Media / Q000737:chemistry; D005260:Female; D058185:Magnetite Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D051379:Mice; D008807:Mice, Inbred BALB C; D015336:Molecular Probe Techniques; D015335:Molecular Probes / Q000737:chemistry; D064847:Multimodal Imaging / Q000379:methods; D053769:Nanocapsules / Q000737:chemistry / Q000648:ultrastructure; D058726:Nanoconjugates / Q000737:chemistry / Q000648:ultrastructure; D054118:Nanospheres / Q000737:chemistry* / Q000648:ultrastructure; D010190:Pancreatic Neoplasms / Q000000981:diagnostic imaging* / Q000378:metabolism* / Q000473:pathology; D010456:Peptides, Cyclic / Q000737:chemistry / Q000493:pharmacokinetics*; D016062:Porosity; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D003287:Contrast Media; C533969:LyP-1 peptide; D058185:Magnetite Nanoparticles; D015335:Molecular Probes; D053769:Nanocapsules; D058726:Nanoconjugates; D010456:Peptides, Cyclic
keywords: LyP-1; Magnetic resonance imaging; Molecular imaging; Pancreatic cancer; Superparamagnetic nanospheres