title: The Life Cycle of Didymium laxifilum and Physarum album on Oat Agar Culture.
abstract: The plasmodial slime molds is the largest group in the phylum Amoebozoa. Its life cycle includes the plasmodial trophic stage and the spore-bearing fruiting bodies. However, only a few species have their complete life cycle known in details so far. This study is the first reporting the morphogenesis of Didymium laxifilum and Physarum album. Spores, from field-collected sporangia, were incubated into hanging drop cultures for viewing germination and axenic oat agar plates for viewing plasmodial development and sporulation. The spores of D. laxifilum and P. album germinated by method of V-shape split and minute pore, respectively. The amoeboflagellates, released from spores, were observed in water film. The phaneroplasmodia of two species developed into a number of sporangia by subhypothallic type on oat agar culture. The main interspecific difference of morphogenesis was also discussed.
authors.affiliation: College of Life Sciences, Nanjing Normal University, Nanjing, Jiangsu, 210023, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Gao
authors.affiliation: College of Life Sciences, Nanjing Normal University, Nanjing, Jiangsu, 210023, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Tao
authors.affiliation: College of Life Sciences, Nanjing Normal University, Nanjing, Jiangsu, 210023, China.
authors.forename: Shu-Zhen
authors.initials: SZ
authors.lastname: Yan
authors.affiliation: College of Life Sciences, Nanjing Normal University, Nanjing, Jiangsu, 210023, China.
authors.forename: Shuang-Lin
authors.initials: SL
authors.lastname: Chen
mesh_terms: D061245:Axenic Culture; D008018:Life Cycle Stages / Q000502:physiology*; D008855:Microscopy, Electron, Scanning; D009024:Morphogenesis; D009235:Myxomycetes / Q000145:classification / Q000254:growth & development*; D010804:Physarum / Q000145:classification / Q000254:growth & development*; D033761:Spores, Protozoan / Q000254:growth & development; D014934:Wood / Q000469:parasitology
keywords: Morphogenesis; myxomycetes; plasmodia; spore-to-spore culture; sporulation