title: Gastrodia elata Blume Extract Modulates Antioxidant Activity and Ultraviolet A-Irradiated Skin Aging in Human Dermal Fibroblast Cells.
abstract: Gastrodia elata Blume (GEB), a traditional herbal medicine, has been used to treat a wide range of neurological disorders (e.g., paralysis and stroke) and skin problems (e.g., atopic dermatitis and eczema) in oriental medicine. This study was designed to investigate the antioxidant ability of GEB and its antiaging effect on human dermal fibroblast cells (HDF). The total phenolic and flavonoid contents of GEB were 21.8 and 0.43 mg/g dry weight (DW), respectively. The ergothioneine content of GEB was 0.41 mg/mL DW. The DPPH and ABTS radical scavenging activities of GEB at 5 and 10 mg/mL approximately ranged between 31% and 44%. The superoxide dismutase activity of GEB at 10 and 25 mg/mL was 57% and 76%, respectively. GEB increased procollagen type 1 (PC1) production and inhibited matrix metalloproteinase-1 (MMP-1) production and elastase-1 activity in UVA-irradiated HDF. PC1 messenger RNA (mRNA) levels decreased upon UVA irradiation, but recovered in response to high doses of GEB in HDF. On the contrary, GEB significantly decreased MMP-1 and elastase-1 mRNA levels, which were markedly induced in UVA-irradiated HDF. Collectively, these results suggest that GEB has sufficient antioxidant ability to prevent the signs of skin aging in UVA-irradiated human skin cells, suggesting its potential as a natural antiaging product.
authors.affiliation: 1 Department of Food and Nutrition, College of Natural Sciences, Myongji University , Yongin, Korea.
authors.forename: Eunju
authors.initials: E
authors.lastname: Song
authors.affiliation: 2 Department of Food and Nutrition, College of Natural Sciences, Soongeui Women's College , Seoul, Korea.
authors.forename: Haeyon
authors.initials: H
authors.lastname: Chung
authors.affiliation: 2 Department of Food and Nutrition, College of Natural Sciences, Soongeui Women's College , Seoul, Korea.
authors.forename: Eugene
authors.initials: E
authors.lastname: Shim
authors.affiliation: 3 BK Bio Co., Ltd. , Jeju, Korea.
authors.forename: Jung-Ky
authors.initials: JK
authors.lastname: Jeong
authors.affiliation: 3 BK Bio Co., Ltd. , Jeju, Korea.
authors.forename: Bok-Kyung
authors.initials: BK
authors.lastname: Han
authors.affiliation: 3 BK Bio Co., Ltd. , Jeju, Korea.
authors.forename: Hyuk-Joon
authors.initials: HJ
authors.lastname: Choi
authors.affiliation: 1 Department of Food and Nutrition, College of Natural Sciences, Myongji University , Yongin, Korea.
authors.forename: Jinah
authors.initials: J
authors.lastname: Hwang
mesh_terms: D000975:Antioxidants / Q000494:pharmacology*; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D024042:Collagen Type I / Q000378:metabolism; D004789:Enzyme Activation / Q000187:drug effects; D005347:Fibroblasts / Q000187:drug effects / Q000201:enzymology / Q000528:radiation effects; D031668:Gastrodia / Q000737:chemistry*; D006801:Humans; D020781:Matrix Metalloproteinase 1 / Q000378:metabolism; D010196:Pancreatic Elastase / Q000378:metabolism; D010936:Plant Extracts / Q000494:pharmacology*; D012333:RNA, Messenger / Q000378:metabolism; D012867:Skin / Q000166:cytology / Q000187:drug effects* / Q000201:enzymology / Q000528:radiation effects*; D015595:Skin Aging / Q000187:drug effects* / Q000473:pathology; D013482:Superoxide Dismutase / Q000378:metabolism; D014466:Ultraviolet Rays / Q000009:adverse effects
chemical_list: D000975:Antioxidants; D024042:Collagen Type I; D010936:Plant Extracts; D012333:RNA, Messenger; D013482:Superoxide Dismutase; D010196:Pancreatic Elastase; C522360:MMP1 protein, human; D020781:Matrix Metalloproteinase 1
keywords: Gastrodia elata Blume; UVA irradiation; antioxidant; photoaging