title: Mechanism of Corilagin interference with IL-13/STAT6 signaling pathways in hepatic alternative activation macrophages in schistosomiasis-induced liver fibrosis in mouse model.
abstract: This study tried to find the mechanism of Corilagin interference with interleukin (IL)-13/signal transducer and activator of transcription (STAT) 6 signaling pathways in IL-13-activated liver alternative activation macrophages in schistosomiasis-induced liver fibrosis in Balb/c mice. As a result, IL-13 in serum and the mRNA expression of IL-13 Receptor α1, IL-4 Receptor α and downstream mediators supressor of cytokine signaling (SOCS) 1, Kruppel-like factor (KLF) 4, peroxisome proliferator-activated receptor (PPAR) δ in the liver tissue were significantly inhibited by Corilagin (P<0.05 or 0.01). The protein expression of IL-13 Receptor α1, IL-4 Receptor α, SOCS1, KLF4, PPARγ, PPARδ and Phospho-STAT6 (P-STAT6) in Corilagin group were also markedly suppressed when compared with the model group (P<0.05 or 0.01). Furthermore, the inhibitory effect was enhanced when the concentration of Corilagin increased (P<0.05). By hematoxylin and eosin (HE) staining, when compared with the model group, the Corilagin group showed smaller granulomas (P<0.05 or 0.01). The area of positive cells and integrated optical density (IOD) of CD68, CD206 and KLF4 was significantly decreased by Corilagin stained by IHC (P<0.05 or 0.01). In conclusion, Corilagin had potential to relieve hepatic fibrosis caused by egg granuloma in Schistosoma japonicum infection by decreasing the expression of molecules associated with IL-13/STAT6 signaling pathway in liver alternative activation macrophages.
authors.affiliation: Hubei Collaborative Innovation Center for Green Transformation of Bio-Resources, Hubei University, Wuhan 430062, PR China.
authors.forename: Peng
authors.initials: P
authors.lastname: Du
authors.affiliation: Hubei Collaborative Innovation Center for Green Transformation of Bio-Resources, Hubei University, Wuhan 430062, PR China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Ma
authors.affiliation: Guangxi University of Chinese Medicine Pharmaceutical Factory, Nanning 530023, PR China.
authors.forename: Zhi-De
authors.initials: ZD
authors.lastname: Zhu
authors.affiliation: Department of Infectious Diseases, Renmin Hospital, Hubei University of Medicine, Shiyan 442000, PR China.
authors.forename: Gang
authors.initials: G
authors.lastname: Li
authors.affiliation: School of Clinical Medical, Hubei University of Chinese Medicine, Wuhan 430060, PR China.
authors.forename: Yao
authors.initials: Y
authors.lastname: Wang
authors.affiliation: School of Clinical Medical, Guangxi University of Chinese Medicine, Nanning 530001, PR China.
authors.forename: Quan-Qiang
authors.initials: QQ
authors.lastname: Li
authors.affiliation: Department of Vascular Surgery Disease, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, PR China.
authors.forename: Yun-Fei
authors.initials: YF
authors.lastname: Chen
authors.affiliation: School of Clinical Medical, Guangxi University of Chinese Medicine, Nanning 530001, PR China.
authors.forename: Zhen-Zhong
authors.initials: ZZ
authors.lastname: Shang
authors.affiliation: School of Clinical Medical, Hubei University of Chinese Medicine, Wuhan 430060, PR China.
authors.forename: Juan
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Department of Infectious Diseases, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, PR China. Electronic address: leizhao@hust.edu.cn.
authors.forename: Lei
authors.initials: L
authors.lastname: Zhao
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D005786:Gene Expression Regulation / Q000187:drug effects; D005960:Glucosides / Q000494:pharmacology* / Q000627:therapeutic use; D047348:Hydrolyzable Tannins / Q000494:pharmacology* / Q000627:therapeutic use; D018793:Interleukin-13 / Q000378:metabolism*; D000090062:Kruppel-Like Factor 4; D051741:Kruppel-Like Transcription Factors / Q000378:metabolism; D008099:Liver / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D008103:Liver Cirrhosis / Q000188:drug therapy* / Q000209:etiology / Q000276:immunology / Q000473:pathology; D008262:Macrophage Activation / Q000187:drug effects*; D008264:Macrophages / Q000187:drug effects / Q000276:immunology; D008297:Male; D051379:Mice; D050800:STAT6 Transcription Factor / Q000378:metabolism*; D012552:Schistosomiasis / Q000150:complications*; D015398:Signal Transduction / Q000187:drug effects
chemical_list: D005960:Glucosides; D047348:Hydrolyzable Tannins; D018793:Interleukin-13; C000715468:Klf4 protein, mouse; D000090062:Kruppel-Like Factor 4; D051741:Kruppel-Like Transcription Factors; D050800:STAT6 Transcription Factor; C049096:corilagin
keywords: Corilagin; IL-13; Liver fibrosis; M2 macrophage; Schistosomiasis