title: Self-Renewal of Bone Marrow Stem Cells by Nanovesicles Engineered from Embryonic Stem Cells.
abstract: Extracellular vesicles can enhance cell proliferation by stimulating signal transduction and delivering genetic materials, and thus may have applications in regenerative medicine and other therapeutic applications. The processes employed to isolate extracellular vesicles, however, are complex and achieve low yield. To overcome these obstacles, a large-scale, micropore device for generating extracellular vesicle-mimetic nanovesicles that have characteristics similar to those of extracellular vesicles is fabricated. The nanovesicles are generated through the self-assembly capability of cell membrane fragments in an aqueous solution. The nanovesicles enhance the proliferation of murine mesenchymal stem cells (MSCs), stimulate the signal pathway related to cell proliferation, and do not influence the characteristics of murine MSCs. Therefore, these nanovesicles could provide stable MSCs for regenerative medicine and other therapeutic applications.
authors.affiliation: Department of Mechanical Engineering, Pohang University of Science and Technology (POSTECH), 77 Cheongam-Ro, Nam-gu, Pohang, Gyeong-buk, 37673, Republic of Korea.
authors.forename: Wonju
authors.initials: W
authors.lastname: Jo
authors.affiliation: School of Interdisciplinary Bioscience and Bioengineering, POSTECH, 77 Cheongam-Ro, Nam-gu, Pohang, Gyeong-buk, 37673, Republic of Korea.
authors.forename: Dayeong
authors.initials: D
authors.lastname: Jeong
authors.affiliation: School of Interdisciplinary Bioscience and Bioengineering, POSTECH, 77 Cheongam-Ro, Nam-gu, Pohang, Gyeong-buk, 37673, Republic of Korea.
authors.forename: Junho
authors.initials: J
authors.lastname: Kim
authors.affiliation: Department of Mechanical Engineering, Pohang University of Science and Technology (POSTECH), 77 Cheongam-Ro, Nam-gu, Pohang, Gyeong-buk, 37673, Republic of Korea.|School of Interdisciplinary Bioscience and Bioengineering, POSTECH, 77 Cheongam-Ro, Nam-gu, Pohang, Gyeong-buk, 37673, Republic of Korea.
authors.forename: Jaesung
authors.initials: J
authors.lastname: Park
mesh_terms: D000818:Animals; D001853:Bone Marrow / Q000502:physiology*; D001854:Bone Marrow Cells / Q000502:physiology*; D002454:Cell Differentiation / Q000502:physiology; D049109:Cell Proliferation / Q000502:physiology; D053595:Embryonic Stem Cells / Q000502:physiology*; D059630:Mesenchymal Stem Cells / Q000502:physiology*; D051379:Mice; D008810:Mice, Inbred C57BL; D036103:Nanotechnology / Q000379:methods; D044968:Regenerative Medicine / Q000379:methods; D015398:Signal Transduction / Q000502:physiology
keywords: extracellular vesicles; mesenchymal stem cells; nanovesicles; regenerative medicine; self-renewal