title: Comparison of autohydrolysis and ionic liquid 1-butyl-3-methylimidazolium acetate pretreatment to enhance enzymatic hydrolysis of sugarcane bagasse.
abstract: The aim of this work was to evaluate the efficiency of an ionic liquid (IL) 1-butyl-3-methylimidazolium acetate ([C4mim][OAc]) pretreatment (110°C for 30min) in comparison to high severity autohydrolysis pretreatment in terms of delignification, cellulose crystallinity and enzymatic digestibility. The increase in severity of autohydrolysis pretreatment had positive effect on glucan digestibility, but was limited by the crystallinity of cellulose. [C4mim][OAc] pretreated sugarcane bagasse exhibited a substantial decrease in lignin content, reduced cellulose crystallinity, and enhanced glucan and xylan digestibility. Glucan and xylan digestibility was determined as 97.4% and 98.6% from [C4mim][OAc] pretreated bagasse, and 62.1% and 57.5% from the bagasse autohydrolyzed at 205°C for 6min, respectively. The results indicated the improved digestibility and hydrolysis rates after [C4mim][OAc] pretreatment when compared against a comparable autohydrolyzed biomass.
authors.affiliation: Department of Chemical and Biomolecular Engineering, The University of Tennessee, Knoxville, TN 37996-2200, USA; Department of Forestry, Wildlife and Fisheries, The University of Tennessee, Knoxville, TN 37996-2200, USA; Department of Microbiology, Faculty of Biological Sciences, Quaid-i-Azam University, Islamabad, Pakistan.
authors.forename: Muzna
authors.initials: M
authors.lastname: Hashmi
authors.affiliation: Department of Chemical and Biomolecular Engineering, The University of Tennessee, Knoxville, TN 37996-2200, USA; Department of Forestry, Wildlife and Fisheries, The University of Tennessee, Knoxville, TN 37996-2200, USA.
authors.forename: Qining
authors.initials: Q
authors.lastname: Sun
authors.affiliation: Center of Renewable Carbon, The University of Tennessee, Knoxville, TN, USA.
authors.forename: Jingming
authors.initials: J
authors.lastname: Tao
authors.affiliation: Department of Chemical and Biomolecular Engineering, The University of Tennessee, Knoxville, TN 37996-2200, USA; Department of Forestry, Wildlife and Fisheries, The University of Tennessee, Knoxville, TN 37996-2200, USA.
authors.forename: Tyrone
authors.initials: T
authors.lastname: Wells
authors.affiliation: Department of Microbiology, Faculty of Biological Sciences, Quaid-i-Azam University, Islamabad, Pakistan.
authors.forename: Aamer Ali
authors.initials: AA
authors.lastname: Shah
authors.affiliation: Department of Forestry, Wildlife and Fisheries, The University of Tennessee, Knoxville, TN 37996-2200, USA; Center of Renewable Carbon, The University of Tennessee, Knoxville, TN, USA.
authors.forename: Nicole
authors.initials: N
authors.lastname: Labbé
authors.affiliation: Department of Chemical and Biomolecular Engineering, The University of Tennessee, Knoxville, TN 37996-2200, USA; Department of Forestry, Wildlife and Fisheries, The University of Tennessee, Knoxville, TN 37996-2200, USA; Center of Renewable Carbon, The University of Tennessee, Knoxville, TN, USA; Joint Institute for Biological Sciences, Biosciences Division, Oak Ridge National Laboratory (ORNL), Oak Ridge, TN 37831, USA. Electronic address: aragausk@utk.edu.
authors.forename: Arthur J
authors.initials: AJ
authors.lastname: Ragauskas
mesh_terms: D018533:Biomass; D001709:Biotechnology / Q000379:methods*; D002482:Cellulose / Q000737:chemistry* / Q000378:metabolism; D004798:Enzymes / Q000737:chemistry / Q000378:metabolism; D005936:Glucans / Q000737:chemistry; D006868:Hydrolysis; D007093:Imidazoles / Q000737:chemistry*; D052578:Ionic Liquids / Q000737:chemistry; D008031:Lignin / Q000737:chemistry; D031786:Saccharum / Q000737:chemistry* / Q000378:metabolism; D017550:Spectroscopy, Fourier Transform Infrared; D014961:X-Ray Diffraction; D014990:Xylans / Q000737:chemistry
chemical_list: C525963:1-butyl-3-methylimidazolium; D004798:Enzymes; D005936:Glucans; D007093:Imidazoles; D052578:Ionic Liquids; D014990:Xylans; D002482:Cellulose; D008031:Lignin; C027433:bagasse
keywords: Autohydrolysis; Hydrolysability; Ionic liquid; Sugarcane bagasse