title: Enhanced fucosylation of GA1 in the digestive tracts of X-ray-irradiated mice.
abstract: In mice at 4 days after X-ray-irradiation at 0.5 Gy/min for 16 min, the tissue weights of immune organs, i.e., thymus and spleen, were decreased due to injury to lymphocytes by the X-rays. The resulting immunosuppressive condition allowed the growth of lactobacilli, i.e., L. murinus, which contained LacβTH-DG and possessed the ability to induce transcription of the fucosyltransferase gene for synthesis of FGA1. LacβTH-DG was detected in the jejunal and ileal contents of X-ray-irradiated mice, but not in those of control mice, whereas LacTetH-DG of L. johnsonii was present in the stomach and caecal contents of both mice. The amounts of FGA1 in the duodenal and jejunal tissues of X-ray-irradiated mice increased to 4- and 9-fold of those in controls, respectively. Reflecting the enhanced fucosylation of GA1, the total amounts of FGA1 excreted into the contents of X-ray-irradiated mice were 1.4-times higher than those in controls. Also, when the extent of enhanced fucosylation of GA1 in several regions of the digestive tracts of X-ray-irradiated mice was compared with that in immune deficient nude, scid and pIgR(-/-) mice, the more than 4-fold increases of FGA1 observed in duodenal and jejunal tissues corresponded to those in pIgR(-/-) mice without secretory IgA. Since an increased amount of FGA1 in the small intestine was observed only 4 days after X-ray-irradiation, and diminished synthesis of FGA1 occurred on administration of penicillin and streptomycin, fucosylation of GA1 in the small intestine was revealed to occur quickly in response to a change in the intestinal bacterial population.
authors.affiliation: Faculty of Science and Technology, Kinki University, 3-4-1 Kowakae, Higashiosaka, Osaka, 577-8502, Japan. iwamori@life.kindai.ac.jp.
authors.forename: Masao
authors.initials: M
authors.lastname: Iwamori
authors.affiliation: Department of Obstetrics and Gynecology, School of Medicine, Keio University, 35 Shinanomachi, Shinjuku-ku, Tokyo, 160-8582, Japan.
authors.forename: Kyoko
authors.initials: K
authors.lastname: Tanaka
authors.affiliation: Animal Models of Human Diseases, National Institute of Biomedical Innovation, 7-6-8 Asagi-Saito, Osaka, Ibaraki, 567-0085, Japan.
authors.forename: Shigeki
authors.initials: S
authors.lastname: Adachi
authors.affiliation: Department of Obstetrics and Gynecology, School of Medicine, Keio University, 35 Shinanomachi, Shinjuku-ku, Tokyo, 160-8582, Japan.
authors.forename: Daisuke
authors.initials: D
authors.lastname: Aoki
authors.affiliation: Animal Models of Human Diseases, National Institute of Biomedical Innovation, 7-6-8 Asagi-Saito, Osaka, Ibaraki, 567-0085, Japan.
authors.forename: Taisei
authors.initials: T
authors.lastname: Nomura
mesh_terms: D000818:Animals; D001419:Bacteria / Q000254:growth & development; D005260:Female; D005643:Fucose / Q000378:metabolism*; D005677:G(M1) Ganglioside / Q000378:metabolism*; D005753:Gastric Mucosa / Q000378:metabolism* / Q000382:microbiology; D000069196:Gastrointestinal Microbiome / Q000528:radiation effects; D007413:Intestinal Mucosa / Q000378:metabolism* / Q000382:microbiology; D051379:Mice; D008819:Mice, Nude; D011833:Radiation Injuries, Experimental / Q000378:metabolism* / Q000382:microbiology; D014965:X-Rays
chemical_list: D005643:Fucose; D005677:G(M1) Ganglioside; C018835:asialo GM1 ganglioside
keywords: Bacterial glycolipid; Digestive tract; Fucosyl GA1; Intestinal bacteria; TLC-immunostaining