title: Evaluation of the modified carbapenem inactivation method and sodium mercaptoacetate-combination method for the detection of metallo-β-lactamase production by carbapenemase-producing Enterobacteriaceae.
abstract: We evaluated the effectiveness of carbapenem inactivation method (CIM) and modified CIM (mCIM). Our results indicated that mCIM with 4h incubation improved sensitivity and specificity for detecting carbapenemase-producing Enterobacteriaceae compared to CIM. Additionally, we developed a sodium mercaptoacetate-combination method (SMA-mCIM) to detect metallo-β-lactamase (MBL) with high sensitivity and specificity.
authors.affiliation: Department of Clinical Laboratory, Tokyo Metropolitan Health and Medical Treatment Corporation Toshima Hospital, Tokyo, Japan. Electronic address: kageto_yamada@tokyo-hmt.jp.
authors.forename: Kageto
authors.initials: K
authors.lastname: Yamada
authors.affiliation: Department of Clinical Laboratory, Tokyo Metropolitan Health and Medical Treatment Corporation Toshima Hospital, Tokyo, Japan.
authors.forename: Machiko
authors.initials: M
authors.lastname: Kashiwa
authors.affiliation: Department of Clinical Laboratory, Tokyo Metropolitan Health and Medical Treatment Corporation Toshima Hospital, Tokyo, Japan.
authors.forename: Katsumi
authors.initials: K
authors.lastname: Arai
authors.affiliation: Department of Health and Medical Sciences, Shinshu University, Graduate School of Medicine, Japan.
authors.forename: Noriyuki
authors.initials: N
authors.lastname: Nagano
authors.affiliation: Department of Microbiology and Immunology, Graduate School of Health Care Sciences, Tokyo Medical and Dental University, Tokyo, Japan.
authors.forename: Ryoichi
authors.initials: R
authors.lastname: Saito
mesh_terms: D001426:Bacterial Proteins / Q000378:metabolism; D001431:Bacteriological Techniques; D015780:Carbapenems / Q000037:antagonists & inhibitors*; D004755:Enterobacteriaceae / Q000201:enzymology / Q000302:isolation & purification*; D012680:Sensitivity and Specificity; D013864:Thioglycolates / Q000737:chemistry; D001618:beta-Lactamases / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D015780:Carbapenems; D013864:Thioglycolates; C017487:2-mercaptoacetate; D001618:beta-Lactamases; C063912:carbapenemase
keywords: Carbapenem inactivation method; Carbapenemase-producing Enterobacteriaceae; Modified carbapenem inactivation method; Sodium mercaptoacetate