title: Fluorescence spectroscopy of teeth and bones of rats to assess demineralization: In vitro, in vivo and ex vivo studies.
abstract: This study investigated the effects of demineralization on teeth and bones evaluated by fluorescence spectroscopy and micro energy-dispersive X-ray fluorescence spectrometry (μ-EDXRF) in rats. For in vitro study, 20 teeth of Wistar rats were removed and decalcified to evaluate fluorescence. For in vivo study, 10 female Wistar rats aged 6months were randomized into 2 groups: Control Group (C): non-ovariectomized rats; Ovariectomy Group (OV): ovariectomized rats to induce osteoporosis. The fluorescence spectroscopy of the teeth was performed for long-term (until 180days). For ex vivo study, the tooth and femur bone of the Wistar rats were removed at 180days to perform fluorescence spectroscopy using excitation laser at 408 and 532nm and μ-EDXRF for calcium (Ca) and phosphorus (P) analysis. There were no intergroup differences in fluorescence spectra with laser at 408nm (p≥0.05), but there were changes in the fluorescence spectra using laser at 532nm which led to both the wavelength shift and changes in the band area (p<0.05). The concentrations of P and Ca for the dentine and cortical bone, respectively, were significantly reduced in OV (p<0.05). Demineralization leading to loss of tissue quality may be assessed by fluorescence spectroscopy using 532nm laser. These findings corroborate those obtained by μ-EDXRF.
authors.affiliation: Optics Group from São Carlos Institute of Physics (IFSC), University of São Paulo (USP), Av. Trabalhador Sãocarlense, 400 - Centro, CEP 13560-970, São Carlos, SP, Brazil. Electronic address: fer.nanda.rp@hotmail.com.
authors.forename: Fernanda Rossi
authors.initials: FR
authors.lastname: Paolillo
authors.affiliation: Optics Group from São Carlos Institute of Physics (IFSC), University of São Paulo (USP), Av. Trabalhador Sãocarlense, 400 - Centro, CEP 13560-970, São Carlos, SP, Brazil. Electronic address: renan.romano@gmail.com.
authors.forename: Renan Arnon
authors.initials: RA
authors.lastname: Romano
authors.affiliation: Optics Group from São Carlos Institute of Physics (IFSC), University of São Paulo (USP), Av. Trabalhador Sãocarlense, 400 - Centro, CEP 13560-970, São Carlos, SP, Brazil. Electronic address: luciana.de.matos@gmail.com.
authors.forename: Luciana
authors.initials: L
authors.lastname: de Matos
authors.affiliation: Optics Group from São Carlos Institute of Physics (IFSC), University of São Paulo (USP), Av. Trabalhador Sãocarlense, 400 - Centro, CEP 13560-970, São Carlos, SP, Brazil. Electronic address: phamilla@ifsc.usp.br.
authors.forename: Phamilla Gracielli Sousa
authors.initials: PG
authors.lastname: Rodrigues
authors.affiliation: Optics Group from São Carlos Institute of Physics (IFSC), University of São Paulo (USP), Av. Trabalhador Sãocarlense, 400 - Centro, CEP 13560-970, São Carlos, SP, Brazil; Biotechnology Program, Federal University of São Carlos (UFSCar), Brazil. Electronic address: vhpanhoca@msn.com.
authors.forename: Vitor Hugo
authors.initials: VH
authors.lastname: Panhóca
authors.affiliation: Laboratory of Biomedical Spectroscopy (LEVB), University of Vale do Paraíba (UNIVAP), Av. Shishima Hifumi, 2911, CEP 12.244-000, São José dos Campos, SP, Brazil. Electronic address: amartin@univap.br.
authors.forename: Airton Abrahão
authors.initials: AA
authors.lastname: Martin
authors.affiliation: Laboratory of Biomedical Spectroscopy (LEVB), University of Vale do Paraíba (UNIVAP), Av. Shishima Hifumi, 2911, CEP 12.244-000, São José dos Campos, SP, Brazil. Electronic address: lesoares@univap.br.
authors.forename: Luis Eduardo Silva
authors.initials: LE
authors.lastname: Soares
authors.affiliation: Optics Group from São Carlos Institute of Physics (IFSC), University of São Paulo (USP), Av. Trabalhador Sãocarlense, 400 - Centro, CEP 13560-970, São Carlos, SP, Brazil. Electronic address: jarbas@ifsc.usp.br.
authors.forename: Jarbas Caiado
authors.initials: JC
authors.lastname: de Castro Neto
authors.affiliation: Optics Group from São Carlos Institute of Physics (IFSC), University of São Paulo (USP), Av. Trabalhador Sãocarlense, 400 - Centro, CEP 13560-970, São Carlos, SP, Brazil. Electronic address: vander@ifsc.usp.br.
authors.forename: Vanderlei Salvador
authors.initials: VS
authors.lastname: Bagnato
mesh_terms: D000818:Animals; D018488:Bone Demineralization, Pathologic; D066298:In Vitro Techniques; D051381:Rats; D013050:Spectrometry, Fluorescence / Q000379:methods*; D017001:Tooth Demineralization