title: The role of S100P and IMP3 in the cytologic diagnosis of pancreatic adenocarcinoma.
abstract: OBJECTIVE
  The aim of this study was to assess the role of the two markers, S100P and IMP3, in differentiating between pancreatic ductal adenocarcinoma (PDA) and non-neoplastic pancreatic tissue in (fine needle aspiration cytology) FNAC.
  
  
  METHODS
  This is a retrospective study that included 72 cases presented with pancreatic mass, where endoscopic guided FNAC was taken from pancreatic lesions. The final histopathologic diagnosis was considered the gold standard. Cell blocks were stained with anti S100P, and IMP3. Nuclear immunoreactivity with or without cytoplasmic staining for the first marker, and cytoplasmic staining for the second marker was considered specific. Sensitivity, specificity, positive predictive value (PPV), negative predictive value (NPV), and total accuracy of the two markers, as well as the combined accuracy of both markers were calculated.
  
  
  RESULTS
  S100P achieved 96.4% sensitivity, 93.3% specificity, 98.2% PPV, 87.5% NPV and 95.8% total accuracy, while IMP3 achieved 91.2% sensitivity, 86.7% specificity, 96.2% PPV, 72.2% NPV and 90.3% total accuracy for PDA. Both markers showed a total combined accuracy of 89%. S100P showed strong and diffuse staining pattern in most of cases, while the staining pattern for IMP3 was moderate and focal in most of cases.
  
  
  CONCLUSIONS
  Both markers were sensitive and specific for diagnosis of PDA. The staining pattern for S100P was easier to evaluate than IMP3.
authors.affiliation: Pathology Department, Cytopathology Unit, NCI, Cairo University, Egypt. Electronic address: Noha781973@yahoo.com.
authors.forename: Noha E
authors.initials: NE
authors.lastname: Ezzat
authors.affiliation: Pathology Department, Cytopathology Unit, NCI, Cairo University, Egypt.
authors.forename: Neveen S
authors.initials: NS
authors.lastname: Tahoun
authors.affiliation: Medical Oncology Department, NCI, Cairo University, Egypt.
authors.forename: Yahia M
authors.initials: YM
authors.lastname: Ismail
mesh_terms: D000230:Adenocarcinoma / Q000175:diagnosis* / Q000235:genetics / Q000473:pathology; D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000096:biosynthesis* / Q000235:genetics; D044963:Biopsy, Fine-Needle; D002135:Calcium-Binding Proteins / Q000096:biosynthesis* / Q000235:genetics; D021441:Carcinoma, Pancreatic Ductal / Q000175:diagnosis* / Q000235:genetics / Q000473:pathology; D003581:Cytodiagnosis / Q000379:methods; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000096:biosynthesis* / Q000235:genetics; D010179:Pancreas / Q000378:metabolism / Q000473:pathology; D016601:RNA-Binding Proteins / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D014408:Biomarkers, Tumor; D002135:Calcium-Binding Proteins; C498780:IGF2BP3 protein, human; D009363:Neoplasm Proteins; D016601:RNA-Binding Proteins; C103003:S100P protein, human
keywords: Cell block; IMP3; Pancreatic adenocarcinoma; S100