title: PRKAG3 polymorphisms associated with sporadic Wolff-Parkinson-White syndrome among a Taiwanese population.
abstract: BACKGROUND
  The aim of this study was to investigate whether mutation in AMP-activated protein kinase (AMPK) subunit genes (PRKAG3-230) is associated with sporadic, isolated Wolff-Parkinson-White (WPW) syndrome.
  
  
  METHODS
  This study consisted of 87 patients with symptomatic WPW syndrome and 93 healthy controls. PRKAG3-230 genotypes were determined using real-time polymerase chain reaction assay. Genotype and allele frequencies of PRKAG3-230 between patients with WPW syndrome and healthy controls were ascertained using chi-square test or Fisher exact test when appropriate.
  
  
  RESULTS
  PRKAG3-230 were genotyped in 87 patients (53 men and 34 women; age=24.4±18.0 years) with WPW syndrome and 93 healthy controls (57 men and 36 women; age=16.8±4.2 years). There were no significant differences between the two groups in terms of age and sex. The patients with CG and CG+CC genotypes had a significantly increased risk of WPW syndrome compared with those with GG genotype [odds ratio (OR)=1.99, 95% confidence interval (CI)=1.01-3.89, p=0.045; OR=1.99, 95% CI=1.04-3.78, p=0.037, respectively]. The allelic types were not associated with the risk of WPW syndrome. The patients with manifest type with CG and CG+CC genotypes had a significantly increased risk of WPW syndrome compared with those with GG genotype (OR=2.86, 95% CI=1.16-7.05, p=0.022; OR=2.84, 95% CI=1.19-6.80, p=0.019, respectively). The patients with right-side accessory pathways with CG and CG+CC genotypes had a significantly increased risk of WPW syndrome compared with those with GG genotype (OR=3.07, 95% CI=1.25-7.51, p=0.014; OR=2.84, 95% CI=1.19-6.80, p=0.019, respectively). The allelic types were not associated with the risk of WPW types and locations.
  
  
  CONCLUSIONS
  This study shows that PRKAG3-230 may be associated with sporadic WPW syndrome among a Taiwanese population. Further studies are warranted to elucidate the role of mutations in AMPK subunit genes other than PRKAG3-230 in sporadic WPW syndrome.
authors.affiliation: Department of Pediatrics, Kaohsiung Veterans General Hospital, Kaohsiung, Taiwan, ROC; Faculty of Medicine, National Yang-Ming University, Taipei, Taiwan, ROC. Electronic address: kenpenweng@yahoo.com.tw.
authors.forename: Ken-Pen
authors.initials: KP
authors.lastname: Weng
authors.affiliation: Department of Pediatrics, Cheng Hsin General Hospital, Taipei, Taiwan, ROC.
authors.forename: Yeong-Seng
authors.initials: YS
authors.lastname: Yuh
authors.affiliation: Department of Nursing, Fooyin University, Kaohsiung, Taiwan, ROC.
authors.forename: Shih-Hui
authors.initials: SH
authors.lastname: Huang
authors.affiliation: Division of Cardiology, Kaohsiung Veterans General Hospital, Kaohsiung, Taiwan, ROC.
authors.forename: Hsiang-Chiang
authors.initials: HC
authors.lastname: Hsiao
authors.affiliation: Department of Pediatrics, Kaohsiung Armed Forces General Hospital, Kaohsiung, Taiwan, ROC.
authors.forename: Huang-Wei
authors.initials: HW
authors.lastname: Wu
authors.affiliation: Department of Pediatrics, Zuoying Branch of Kaohsiung Armed Forces General Hospital, Kaohsiung, Taiwan, ROC.
authors.forename: Jen-Hung
authors.initials: JH
authors.lastname: Chien
authors.affiliation: Department of Pediatrics, Taoyuan Armed Forces General Hospital, Taoyuan, Taiwan, ROC.
authors.forename: Bo-Hau
authors.initials: BH
authors.lastname: Chen
authors.affiliation: Department of Pediatrics, Kaohsiung Municipal United Hospital, Kaohsiung, Taiwan, ROC.
authors.forename: Shih-Ming
authors.initials: SM
authors.lastname: Huang
authors.affiliation: Department of Pediatrics, Kaohsiung Veterans General Hospital, Kaohsiung, Taiwan, ROC.
authors.forename: Kuang-Jen
authors.initials: KJ
authors.lastname: Chien
authors.affiliation: Department of Medical Education and Research, Kaohsiung Veterans General Hospital, Kaohsiung, Taiwan, ROC; Institute of Biomedical Sciences, National Sun Yat-Sen University, Kaohsiung, Taiwan, ROC.
authors.forename: Luo-Ping
authors.initials: LP
authors.lastname: Ger
mesh_terms: D055372:AMP-Activated Protein Kinases / Q000235:genetics*; D000293:Adolescent; D000328:Adult; D002648:Child; D005787:Gene Frequency; D005838:Genotype; D006801:Humans; D008297:Male; D014927:Wolff-Parkinson-White Syndrome / Q000235:genetics*; D055815:Young Adult
chemical_list: C526225:PRKAG3 protein, human; D055372:AMP-Activated Protein Kinases
keywords: PRKAG3-230 (rs692243); Wolff–Parkinson–White syndrome; genetics; tachycardia