title: Metabolic characterization of the early stage of hepatic fibrosis in rat using GC-TOF/MS and multivariate data analyses.
abstract: The aim of this study was to explore the changes in the urine metabolic spectrum in rats with the early stage of liver fibrosis using gas chromatography-time of flight/mass spectrometry (GC-TOF/MS), try to search for potential biomarkers and elucidate the probably metabonomic pathogenesis. The early stage of liver fibrosis was established with a single subcutaneous injection of carbon tetrachloride twice each week for 4 weeks continuously. At the end of the experiment, GC-TOF/MS technology with multivariate statistical approaches such as principal component analysis, partial least squares-discriminant analysis and orthogonal partial least squares-discriminant analysis was used to analyze the changes in the metabolic spectrum trajectory and identify potential biomarkers. Twelve potential biomarkers in the model group, such as succinic acid, threonine and lactose, were selected, which indicate that the metabonomic pathogenesis of the early stage of liver fibrosis may be related to disorders of energy metabolism, amino acid metabolism and fatty acid metabolism.
authors.affiliation: Department of Pharmacy, The first affiliated hospital of Anhui university of Chinese medicine, Hefei, China.|College of Basic Medicine, Anhui Medical University, Hefei, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Jiang
authors.affiliation: Department of Pharmacy, The first affiliated hospital of Anhui university of Chinese medicine, Hefei, China.
authors.forename: Jun-Mei
authors.initials: JM
authors.lastname: Song
authors.affiliation: College of Pharmacy, Dali University, Dali, China.
authors.forename: Peng-Fei
authors.initials: PF
authors.lastname: Gao
authors.affiliation: Department of Pharmacy, The first affiliated hospital of Anhui university of Chinese medicine, Hefei, China.
authors.forename: Xiu-Juan
authors.initials: XJ
authors.lastname: Qin
authors.affiliation: Department of Pharmacy, The first affiliated hospital of Anhui university of Chinese medicine, Hefei, China.
authors.forename: Shuang-Zhi
authors.initials: SZ
authors.lastname: Xu
authors.affiliation: Department of Pharmacy, The first affiliated hospital of Anhui university of Chinese medicine, Hefei, China.
authors.forename: Jia-Fu
authors.initials: JF
authors.lastname: Zhang
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000652:urine; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D008099:Liver / Q000473:pathology; D008103:Liver Cirrhosis / Q000378:metabolism* / Q000473:pathology; D008297:Male; D015999:Multivariate Analysis; D025341:Principal Component Analysis; D051381:Rats; D017207:Rats, Sprague-Dawley; D016482:Urinalysis
chemical_list: D015415:Biomarkers
keywords: GC-TOF/MS; metabolomics; potential biomarker; the early stage of liver fibrosis; urine