title: Association of a TFAM haplotype with aggressive prostate cancer in overweight or obese Mexican Mestizo men.
abstract: Mitochondrial dysfunction has been associated with the development of cancer and obesity, being prostate cancer more aggressive in obese men. It has been suggested that the mitochondrial transcription factor A (TFAM) plays a central role in these events.
  
  
  
  The aim of this study was to analyze the possible association of 3 TFAM polymorphisms, as well as their haplotypes, with the development of aggressive prostate cancer in overweight or obese Mexican Mestizo men.
  
  
  
  A total of 257 unrelated men with histologically confirmed prostate cancer, of Mexican Mestizo ethnic origin, were included. Body mass index was determined and the degree of prostate cancer aggressiveness was demarcated by the D'Amico classification. DNA was obtained from blood leukocytes. The rs1937, rs1049432, and rs11006132, as well as their haplotypes, were studied by real-time polymerase chain reaction allelic discrimination. Deviations from Hardy-Weinberg equilibrium were tested. Pairwise linkage disequilibrium between single nucleotide polymorphisms was calculated; haplotype analysis was performed.
  
  
  
  A higher risk (D'Amico classification) was documented in 56 patients (21.8%). We did not find a significant association among those polymorphisms analyzed; however, one haplotype was significantly associated with cancer aggressiveness.
  
  
  
  To our knowledge, this constitutes the first study regarding the relationship of 3 TFAM polymorphisms, as well as their haplotypes, and the aggressiveness of prostate cancer in overweight or obese men; the most frequent haplotype was associated with cancer aggressiveness.
authors.affiliation: Unidad de Investigación en Obesidad, Facultad de Medicina, Universidad Nacional Autónoma de México, México D.F., Mexico.
authors.forename: Jesús Benítez
authors.initials: JB
authors.lastname: Granados
authors.affiliation: Unidad de Investigación en Obesidad, Facultad de Medicina, Universidad Nacional Autónoma de México, México D.F., Mexico; Clínica de Obesidad, Instituto Nacional de Ciencias Médicas y Nutrición "Salvador Zubirán," México D.F., Mexico.
authors.forename: Juan Pablo
authors.initials: JP
authors.lastname: Méndez
authors.affiliation: Departamento de Urología, Instituto Nacional de Ciencias Médicas y Nutrición "Salvador Zubirán," México D.F., Mexico.
authors.forename: Guillermo
authors.initials: G
authors.lastname: Feria-Bernal
authors.affiliation: Clínica de Obesidad, Instituto Nacional de Ciencias Médicas y Nutrición "Salvador Zubirán," México D.F., Mexico.
authors.forename: Eduardo
authors.initials: E
authors.lastname: García-García
authors.affiliation: Unidad de Investigación en Obesidad, Facultad de Medicina, Universidad Nacional Autónoma de México, México D.F., Mexico.
authors.forename: María Elena
authors.initials: ME
authors.lastname: Tejeda
authors.affiliation: Unidad de Medicina Física y Rehabilitación Centro, UMAE, Hospital de Traumatología y Ortopedia "Lomas Verdes," Instituto Mexicano del Seguro Social, México D.F., Mexico.
authors.forename: David
authors.initials: D
authors.lastname: Rojano-Mejía
authors.affiliation: Unidad de Investigación en Obesidad, Facultad de Medicina, Universidad Nacional Autónoma de México, México D.F., Mexico.
authors.forename: André
authors.initials: A
authors.lastname: Tapia
authors.affiliation: Unidad de Investigación en Obesidad, Facultad de Medicina, Universidad Nacional Autónoma de México, México D.F., Mexico; Clínica de Obesidad, Instituto Nacional de Ciencias Médicas y Nutrición "Salvador Zubirán," México D.F., Mexico. Electronic address: ipcanto@yahoo.com.mx.
authors.forename: Patricia
authors.initials: P
authors.lastname: Canto
mesh_terms: D000230:Adenocarcinoma / Q000097:blood / Q000235:genetics* / Q000473:pathology*; D000368:Aged; D000483:Alleles; D015992:Body Mass Index; D003430:Cross-Sectional Studies; D004247:DNA / Q000302:isolation & purification; D004268:DNA-Binding Proteins / Q000235:genetics*; D005787:Gene Frequency; D006239:Haplotypes; D006801:Humans; D007610:Kallikreins / Q000097:blood; D007962:Leukocytes; D015810:Linkage Disequilibrium; D008297:Male; D008800:Mexico; D008875:Middle Aged; D008928:Mitochondria / Q000473:pathology; D024101:Mitochondrial Proteins / Q000235:genetics*; D060787:Neoplasm Grading; D009367:Neoplasm Staging; D050177:Overweight / Q000097:blood / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide; D011467:Prostate / Q000473:pathology; D017430:Prostate-Specific Antigen / Q000097:blood; D011471:Prostatic Neoplasms / Q000097:blood / Q000235:genetics* / Q000473:pathology*; D060888:Real-Time Polymerase Chain Reaction; D012189:Retrospective Studies; D012307:Risk Factors; D017422:Sequence Analysis, DNA; D014157:Transcription Factors / Q000235:genetics*
chemical_list: D004268:DNA-Binding Proteins; D024101:Mitochondrial Proteins; C499631:TFAM protein, human; D014157:Transcription Factors; D004247:DNA; C549870:KLK3 protein, human; D007610:Kallikreins; D017430:Prostate-Specific Antigen
keywords: Aggressiveness; Obesity; Overweight; Polymorphisms; Prostate cancer; TFAM