title: Preclinical imaging anesthesia in rodents.
abstract: Despite the outstanding progress achieved by preclinical imaging science, laboratory animal anesthesia remains quite stationary. Ninety percent of preclinical imaging studies are carried on small rodents (mice and rats) anesthetized by outdated injectable and/or inhalation agents. A need for imaging awake (conscious) animals is questionably registered mainly for brain research, for phMRI and for accomplishing pain and analgesia studies. A need for improving current rodent anesthesia protocols and for enforcing the 3Rs paradigm is sought. Patient monitoring throughout the procedure and recovery phases, as well as vital parameter's data must be recorded in basic consciousness states and during imaging sessions. A multidrug approach is suggested to overcome the limits of monoanesthesia and well-timed physiological data are required to ground findings and to interpret imaging data.
authors.affiliation: Department of Veterinary Medicine and Animal Production, Federico II University, Naples, Italy - gvesce@unina.it.
authors.forename: Giancarlo
authors.initials: G
authors.lastname: Vesce
authors.affiliation: Department of Veterinary Medicine and Animal Production, Federico II University, Naples, Italy.
authors.forename: Fabiana
authors.initials: F
authors.lastname: Micieli
authors.affiliation: Ryan Veterinary Hospital, University of Pennsylvania, Philadelphia, PA, USA.
authors.forename: Ludovica
authors.initials: L
authors.lastname: Chiavaccini
mesh_terms: D000758:Anesthesia / Q000009:adverse effects / Q000379:methods*; D000777:Anesthetics / Q000009:adverse effects / Q000494:pharmacology; D000818:Animals; D003952:Diagnostic Imaging / Q000379:methods*; D006801:Humans; D012377:Rodentia
chemical_list: D000777:Anesthetics