title: Carbon nanotube-polymer composite for effervescent pipette tip solid phase microextraction of alkaloids and flavonoids from Epimedii herba in biological samples.
abstract: An effervescent pipette tip solid phase microextraction based on carbon nanotube-polymer composite microspheres was developed for the simultaneous extraction and determination of four alkaloids (magnoflorine, epiberberine, palmatine, and jatrorrhizine) and four flavonoids (epimedin A/B/C and icariin) from Epimedii herba in biological samples. In this work, the polymeric sorbent was prepared by incorporating carbon nanotube in sulfonated polystyrene-divinylbenzene microspheres. During the extraction, the dispersion of the sorbents at milligram level was achieved by the in situ generation of carbon dioxide. The effervescence enhanced the interaction between the sorbent and the analytes. Langmuir and Freundlich models were used to evaluate adsorption processes. Under the optimum analytical conditions, the method showed good linearity (3-300μgL-1), acceptable precision (RSD <5%), low limits of quantification (1.02-2.98μgL-1) and satisfactory recoveries (90.05-99.85%). The proposed method was successfully applied to the analysis of alkaloids and flavonoids from Epimedii herba in cell culture fluids, rat urine and feces obtained at the different time intervals.
authors.affiliation: Department of Pharmacognosy, Second Military Medical University, School of Pharmacy, Shanghai 200433, China; Department of Medicine, Zhejiang Academy of Traditional Chinese Medicine, Hangzhou, Zhejiang 310007, China.
authors.forename: Nani
authors.initials: N
authors.lastname: Wang
authors.affiliation: Department of Pharmacognosy, Second Military Medical University, School of Pharmacy, Shanghai 200433, China.
authors.forename: Hailiang
authors.initials: H
authors.lastname: Xin
authors.affiliation: Department of Pharmacognosy, Second Military Medical University, School of Pharmacy, Shanghai 200433, China.
authors.forename: Qiaoyan
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: Department of Pharmacognosy, Second Military Medical University, School of Pharmacy, Shanghai 200433, China.
authors.forename: Yiping
authors.initials: Y
authors.lastname: Jiang
authors.affiliation: Department of Medicine, Zhejiang Academy of Traditional Chinese Medicine, Hangzhou, Zhejiang 310007, China.
authors.forename: Xuping
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Medicine, Zhejiang Academy of Traditional Chinese Medicine, Hangzhou, Zhejiang 310007, China. Electronic address: shoudanok@163.com.
authors.forename: Dan
authors.initials: D
authors.lastname: Shou
authors.affiliation: Department of Pharmacognosy, Second Military Medical University, School of Pharmacy, Shanghai 200433, China. Electronic address: lpqin@smmu.edu.cn.
authors.forename: Luping
authors.initials: L
authors.lastname: Qin
mesh_terms: D000470:Alkaloids / Q000032:analysis* / Q000302:isolation & purification / Q000652:urine; D000818:Animals; D002478:Cells, Cultured; D017077:Culture Media, Conditioned / Q000737:chemistry; D034181:Epimedium / Q000737:chemistry*; D005243:Feces / Q000737:chemistry; D005260:Female; D005419:Flavonoids / Q000032:analysis* / Q000302:isolation & purification / Q000652:urine; D051379:Mice; D037742:Nanotubes, Carbon / Q000737:chemistry*; D010006:Osteoblasts / Q000737:chemistry; D010010:Osteoclasts / Q000737:chemistry; D011108:Polymers / Q000737:chemistry*; D017208:Rats, Wistar; D015203:Reproducibility of Results; D052617:Solid Phase Microextraction / Q000295:instrumentation / Q000379:methods*
chemical_list: D000470:Alkaloids; D017077:Culture Media, Conditioned; D005419:Flavonoids; D037742:Nanotubes, Carbon; D011108:Polymers
keywords: Biological sample; Carbon nanotube; Effervescent; Solid phase microextraction; Traditional Chinese medicine