title: CD47 is a Potential Target for the Treatment of Laryngeal Squamous Cell Carcinoma.
abstract: OBJECTIVE
  This study aims to investigate the effect of CD47 on the development of laryngeal squamous cell carcinoma (LSCC) and the therapeutic potential of monoclonal antibody against CD47 and its ligand SIRPα in the treatment of LSCC.
  
  
  METHODS
  We firstly detected the expressions of CD47 mRNA and protein in LSCC and para-carcinoma tissues, introduced the most efficient CD47siRNA sequence into LSCC cells by lentiviral transfection and employed three monoclonal antibodies to evaluate their anti-LSCC effects in vitro and in vivo.
  
  
  RESULTS
  We observed that the mRNA and protein expressions of CD47 in LSCC tissue had significant increase in LSCC tissues compared with those in para-carcinoma tissue (p < 0.05). After the treatments of three monoclonal antibodies, i.e. anti-SIRPα, anti-CD47 BRIC126, anti-CD47 B6H12.2, in rats transfected with Hep-2 cell, it has been showed that the mRNA and protein expressions of CD47 in LSCC tissue decreased, macrophage efficiency was promoted when anti-SIRPα and/or CD47siRNA were used, the amounts, viabilities and expressions of CD47 protein of tumor cell were significantly inhibited. Additionally, combined use of CD47siRNA and anti-SIRPα seemed more efficient than solo use of CD47siRNA/anti-SIRPα.
  
  
  CONCLUSIONS
  The results suggested a critical role of CD47 in LSCC development and the promising treatment of antiCD47/SIRPα and/or CD47siRNA in LSCC.
authors.affiliation: Department of Otorhinolaryngology Head and Neck Surgery, The Second Affiliated Hospital of Nanchang University, Nanchang, China.
authors.forename: ChunPing
authors.initials: C
authors.lastname: Yang
authors.forename: ShuFeng
authors.initials: S
authors.lastname: Gao
authors.forename: HaiZhen
authors.initials: H
authors.lastname: Zhang
authors.forename: Lian
authors.initials: L
authors.lastname: Xu
authors.forename: JianGuo
authors.initials: J
authors.lastname: Liu
authors.forename: Meiqun
authors.initials: M
authors.lastname: Wang
authors.forename: ShaoRong
authors.initials: S
authors.lastname: Zhang
mesh_terms: D000818:Animals; D051928:CD47 Antigen / Q000235:genetics / Q000378:metabolism*; D002294:Carcinoma, Squamous Cell / Q000235:genetics / Q000473:pathology / Q000628:therapy*; D045744:Cell Line, Tumor; D005434:Flow Cytometry; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007822:Laryngeal Neoplasms / Q000235:genetics / Q000473:pathology / Q000628:therapy*; D016086:Lentivirus / Q000378:metabolism; D008264:Macrophages / Q000378:metabolism; D058990:Molecular Targeted Therapy; D010587:Phagocytosis; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D034741:RNA, Small Interfering / Q000378:metabolism; D051381:Rats; D014162:Transfection
chemical_list: D051928:CD47 Antigen; C497894:CD47 protein, human; D012333:RNA, Messenger; D034741:RNA, Small Interfering