title: Methylation-Specific Polymerase Chain Reaction (PCR) for Gene-Specific DNA Methylation Detection.
abstract: Methylation-specific polymerase chain reaction (MS-PCR) is a more rapid way to detect changes in DNA methylation than is bisulfite sequencing. In addition, by incorporating some basic automation, samples can be prepared and analyzed in a 96-well plate format. The method can be used either quantitatively (qRT-PCR-based MethyLight) or qualitatively (using agarose gels) to detect changes in DNA methylation; both are described in this protocol.
authors.forename: Paul M
authors.initials: PM
authors.lastname: Lizardi
authors.forename: Qin
authors.initials: Q
authors.lastname: Yan
authors.forename: Narendra
authors.initials: N
authors.lastname: Wajapeyee
mesh_terms: D004247:DNA / Q000737:chemistry* / Q000235:genetics*; D019175:DNA Methylation; D016133:Polymerase Chain Reaction / Q000379:methods*
chemical_list: D004247:DNA