title: Identification and molecular cloning of glutamate decarboxylase gene fromLactobacillus casei.
abstract: Gamma-amino butyric acid (GABA) possesses several physiological functions such as neurotransmission, induction of hypotension, diuretic and tranquilizer effects. Production of GABA-enriched products by lactic acid bacteria has been a focus of different researches in recent years because of their safety and health-promoting specifities. In this study, glutamate decarboxylase (gad)gene of a local strainsLactobacillus caseiwas identified and cloned. In order to clone thegadgene from this strain, the PCR was carried out using primers designed based on conserved regions. The PCR product was purified and ligated into PGEM-T vector. Comparison of obtained sequences shows that this fragment codes the pyridoxal 5'-phosphate binding region. This strain could possibly be used for the industrial GABA production and also for development of functional fermented foods.Gadgene manipulation can also either decrease or increase the activity of enzyme in bacteria.
authors.affiliation: Department of Biotechnology, Faculty of Advanced Sciences and Technologies, University of Isfahan, Isfahan, Iran.
authors.forename: Yasaman
authors.initials: Y
authors.lastname: Tavakoli
authors.affiliation: Department of Biotechnology, Faculty of Advanced Sciences and Technologies, University of Isfahan, Isfahan, Iran; Department of Biology, Faculty of Sciences, University of Isfahan, Isfahan, Iran.
authors.forename: Abolghasem
authors.initials: A
authors.lastname: Esmaeili
authors.affiliation: Department of Biotechnology, Faculty of Advanced Sciences and Technologies, University of Isfahan, Isfahan, Iran; Department of Biology, Faculty of Sciences, University of Isfahan, Isfahan, Iran.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Rabbani
keywords: Gamma-aminobutyric acid; Glutamate decarboxylase; Lactobacillus casei