title: In vivovolumetric fluorescence sectioning microscopy with mechanical-scan-free hybrid illumination imaging.
abstract: Optical sectioning microscopy in wide-field fashion has been widely used to obtain three-dimensional images of biological samples; however, it requires scanning in depth and considerable time to acquire multiple depth information of a volumetric sample. In this paper,in vivooptical sectioning microscopy with volumetric hybrid illumination, with no mechanical moving parts, is presented. The proposed system is configured such that the optical sectioning is provided by hybrid illumination using a digital micro-mirror device (DMD) for uniform and non-uniform pattern projection, while the depth of imaging planes is varied by using an electrically tunable-focus lens with invariant magnification and resolution. We present and characterize the design, implementation, and experimentally demonstrate the proposed system's ability through 3D imaging ofin vivoCanenorhabditis elegans' growth cones.
authors.affiliation: Department of Electrical Engineering and Graduate Institute of Photonics and Optoelectronics, National Taiwan University, Taipei 10617, Taiwan; Institute of Medical Device and Imaging, National Taiwan University, Taipei, 10051, Taiwan.
authors.forename: Chen-Yen
authors.initials: CY
authors.lastname: Lin
authors.affiliation: Institute of Medical Device and Imaging, National Taiwan University, Taipei, 10051, Taiwan; School of Medicine, National Taiwan University, Taipei, 10051, Taiwan.
authors.forename: Wei-Hsin
authors.initials: WH
authors.lastname: Lin
authors.affiliation: Institute of Medical Device and Imaging, National Taiwan University, Taipei, 10051, Taiwan.
authors.forename: Ju-Hsuan
authors.initials: JH
authors.lastname: Chien
authors.affiliation: Institute of Medical Device and Imaging, National Taiwan University, Taipei, 10051, Taiwan.
authors.forename: Jui-Chang
authors.initials: JC
authors.lastname: Tsai
authors.affiliation: Institute of Medical Device and Imaging, National Taiwan University, Taipei, 10051, Taiwan; Molecular Imaging Center, National Taiwan University, Taipei 10055, Taiwan.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Luo
keywords: (110.0110) Imaging systems; (110.6880) Three-dimensional image acquisition; (180.2520) Fluorescence microscopy