title: In female rat heart mitochondria, oophorectomy results in loss of oxidative phosphorylation.
abstract: Oophorectomy in adult rats affected cardiac mitochondrial function. Progression of mitochondrial alterations was assessed at one, two and three months after surgery: at one month, very slight changes were observed, which increased at two and three months. Gradual effects included decrease in the rates of oxygen consumption and in respiratory uncoupling in the presence of complex I substrates, as well as compromised Ca2+buffering ability. Malondialdehyde concentration increased, whereas the ROS-detoxifying enzyme Mn2+superoxide dismutase (MnSOD) and aconitase lost activity. In the mitochondrial respiratory chain, the concentration and activity of complex I and complex IV decreased. Among other mitochondrial enzymes and transporters, adenine nucleotide carrier and glutaminase decreased. 2-Oxoglutarate dehydrogenase and pyruvate dehydrogenase also decreased. Data strongly suggest that in the female rat heart, estrogen depletion leads to progressive, severe mitochondrial dysfunction.
authors.affiliation: Departamento de FarmacologíaInstituto Nacional de Cardiología Ignacio Chávez, México, Mexico pavitonat@yahoo.com.mx.
authors.forename: Natalia
authors.initials: N
authors.lastname: Pavón
authors.affiliation: Departamento de Genética MolecularInstituto de Fisiología Celular, Universidad Nacional Autónoma de México, México D.F., Mexico.
authors.forename: Alfredo
authors.initials: A
authors.lastname: Cabrera-Orefice
authors.affiliation: Departamento de BioquímicaInstituto Nacional de Cardiología Ignacio Chávez, México, Mexico.
authors.forename: Juan Carlos
authors.initials: JC
authors.lastname: Gallardo-Pérez
authors.affiliation: Departamento de Genética MolecularInstituto de Fisiología Celular, Universidad Nacional Autónoma de México, México D.F., Mexico.
authors.forename: Cristina
authors.initials: C
authors.lastname: Uribe-Alvarez
authors.affiliation: Unidad de Investigación en Reproducción HumanaInstituto Nacional de Perinatología-Facultad de Química UNAM, México D.F., Mexico.
authors.forename: Nadia A
authors.initials: NA
authors.lastname: Rivero-Segura
authors.affiliation: Unidad de Investigación en Reproducción HumanaInstituto Nacional de Perinatología-Facultad de Química UNAM, México D.F., Mexico.
authors.forename: Edgar Ricardo
authors.initials: ER
authors.lastname: Vazquez-Martínez
authors.affiliation: Unidad de Investigación en Reproducción HumanaInstituto Nacional de Perinatología-Facultad de Química UNAM, México D.F., Mexico.
authors.forename: Marco
authors.initials: M
authors.lastname: Cerbón
authors.affiliation: División Académica Multidisciplinaria de ComalcalcoUniversidad Juárez Autónoma de Tabasco, México, Mexico.
authors.forename: Eduardo
authors.initials: E
authors.lastname: Martínez-Abundis
authors.affiliation: Departamento de FarmacologíaInstituto Nacional de Cardiología Ignacio Chávez, México, Mexico.
authors.forename: Juan Carlos
authors.initials: JC
authors.lastname: Torres-Narvaez
authors.affiliation: Departamento de Instrumentación ElectromecánicaInstituto Nacional de Cardiología Ignacio Chávez, Tlalpan DF, México, Mexico.
authors.forename: Raúl
authors.initials: R
authors.lastname: Martínez-Memije
authors.affiliation: Departamento de Consulta externaInstituto Nacional de Cardiología Ignacio Chávez, México, Mexico.
authors.forename: Francisco-Javier
authors.initials: FJ
authors.lastname: Roldán-Gómez
authors.affiliation: Departamento de Genética MolecularInstituto de Fisiología Celular, Universidad Nacional Autónoma de México, México D.F., Mexico.
authors.forename: Salvador
authors.initials: S
authors.lastname: Uribe-Carvajal
mesh_terms: D000154:Aconitate Hydratase / Q000378:metabolism; D000818:Animals; D005260:Female; D008315:Malondialdehyde / Q000378:metabolism; D008929:Mitochondria, Heart / Q000378:metabolism*; D010052:Ovariectomy; D010085:Oxidative Phosphorylation; D010101:Oxygen Consumption / Q000502:physiology*; D051381:Rats; D017382:Reactive Oxygen Species / Q000378:metabolism*; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D017382:Reactive Oxygen Species; D008315:Malondialdehyde; D013482:Superoxide Dismutase; D000154:Aconitate Hydratase
keywords: estrogen receptors; estrogens; gender; heart mitochondria; oophorectomy