title: 13C MR Hyperpolarization of Lactate by Using ParaHydrogen and Metabolic Transformation in Vitro.
abstract: Hyperpolarization of the13C magnetic resonance signal of l-[1-13C]lactate has been obtained using the chemically based, cost-effective method called parahydrogen-induced polarization by means of side-arm hydrogenation (PHIP-SAH). Two ester derivatives of lactate were tested and the factors that determine the polarization level on the product have been investigated in detail. The metabolic conversion of hyperpolarized l-[1-13C]lactate into pyruvate has been observed in vitro using lactate dehydrogenase (LDH) and in a cells lysate. From the acquisition of a series of13C NMR spectra, the metabolic build-up of the [1-13C]pyruvate signal has been observed. These studies demonstrate that, even if the experimental set-up used for these PHIP-SAH hyperpolarization studies is still far from optimal, the attained polarization level is already sufficient to carry out in vitro metabolic studies.
authors.affiliation: Department of Molecular Biotechnology and Health Sciences, University of Torino, via Nizza 52, Torino, Italy.
authors.forename: Eleonora
authors.initials: E
authors.lastname: Cavallari
authors.affiliation: Department of Molecular Biotechnology and Health Sciences, University of Torino, via Nizza 52, Torino, Italy.
authors.forename: Carla
authors.initials: C
authors.lastname: Carrera
authors.affiliation: Department of Molecular Biotechnology and Health Sciences, University of Torino, via Nizza 52, Torino, Italy.|Istituto Bioimmagini e Biostrutture del CNR, Sezione di Torino c/o Center of Molecular Imaging, Via Nizza 52, Torino, Italy.
authors.forename: Silvio
authors.initials: S
authors.lastname: Aime
authors.affiliation: Department of Molecular Biotechnology and Health Sciences, University of Torino, via Nizza 52, Torino, Italy.
authors.forename: Francesca
authors.identifier: http://orcid.org/0000-0002-3318-8367
authors.initials: F
authors.lastname: Reineri
mesh_terms: D066241:Carbon-13 Magnetic Resonance Spectroscopy; D006859:Hydrogen / Q000737:chemistry*; D006865:Hydrogenation; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D019344:Lactic Acid / Q000737:chemistry / Q000378:metabolism; D011773:Pyruvates / Q000737:chemistry / Q000378:metabolism
chemical_list: D011773:Pyruvates; D019344:Lactic Acid; D006859:Hydrogen; D007770:L-Lactate Dehydrogenase
keywords: NMR spectroscopy; lactate dehydrogenase; metabolism; pyruvates