title: Antheraea pernyi silk sericin mediating biomimetic nucleation and growth of hydroxylapatite crystals promoting bone matrix formation.
abstract: Bone biomineralization is well-regulated processes mediated by extracellular matrix proteins. The materials that can direct nucleation of hydroxylapatite (HAp) crystals and assembly of well-structured material-minerals complex are the key to mimicking the natural mineralization. This study used sericin from Antheraea pernyi (A.pernyi), non-mulberry silkworm cocoon as template to mediate nucleation of HAp crystals. Here we find out that AS (Antheraea pernyi sericin) can nucleate the formation HAp crystals in simulated body fluid verified by XRD and FTIR observations. The HAp crystals are organized into nano-rods oriented with c-axis preferentially parallel to the long axis of AS due to hydrogen bonds and electrostatic interaction and finally aggregated into HAp globule. The cell culture of human bone marrow-derived mesenchymal stem cells (BMSCs) showed that the HAp crystals mediated by AS not only stimulate cell adhesion and proliferation but also promote 0f osteogenic differentiation, suggesting that the resultant mineralized AS biomaterial has potential in promoting bone formation. Thus our work will provide significant implication on biomineralization of A. pernyi silk sericin as a potential scaffold for tissue engineering.
authors.affiliation: Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing, 210039, People's Republic of China.
authors.forename: Zhuang
authors.initials: Z
authors.lastname: Jiayao
authors.affiliation: Institute of Applied Bioresources, College of Animal Sciences, Zhejiang University, Hangzhou, 310058, People's Republic of China.
authors.forename: Zhou
authors.initials: Z
authors.lastname: Guanshan
authors.affiliation: Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing, 210039, People's Republic of China.
authors.forename: Zhang
authors.initials: Z
authors.lastname: Jinchi
authors.affiliation: Institute of Applied Bioresources, College of Animal Sciences, Zhejiang University, Hangzhou, 310058, People's Republic of China.
authors.forename: Chen
authors.initials: C
authors.lastname: Yuyin
authors.affiliation: Zhejiang Academy of Traditional Chinese Medicine, Hangzhou, 310007, People's Republic of China.
authors.forename: Zhu
authors.initials: Z
authors.lastname: Yongqiang
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry; D032701:Biomimetics; D012831:Bombyx / Q000502:physiology; D001857:Bone Matrix / Q000187:drug effects / Q000502:physiology; D002113:Calcification, Physiologic; D002118:Calcium / Q000378:metabolism; D002448:Cell Adhesion; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D003460:Crystallization; D017886:Durapatite / Q000737:chemistry* / Q000494:pharmacology; D006801:Humans; D008422:Materials Testing; D059630:Mesenchymal Stem Cells / Q000502:physiology; D009036:Moths / Q000502:physiology*; D010012:Osteogenesis / Q000187:drug effects*; D047030:Sericins / Q000737:chemistry / Q000494:pharmacology*; D017550:Spectroscopy, Fourier Transform Infrared; D023822:Tissue Engineering / Q000379:methods; D014961:X-Ray Diffraction
chemical_list: D001672:Biocompatible Materials; D047030:Sericins; D017886:Durapatite; D000469:Alkaline Phosphatase; D002118:Calcium
keywords: Antheraea pernyi (A.pernyi) sericin; biomineralization; human bone marrow-derived mesenchymal stem cells; hydroxylapatite crystals