title: Differential Expression of NADPH Oxidases Depends on Skeletal Muscle Fiber Type in Rats.
abstract: NADPH oxidases (NOX) are important sources of reactive oxygen species (ROS) in skeletal muscle, being involved in excitation-contraction coupling. Thus, we aimed to investigate if NOX activity and expression in skeletal muscle are fiber type specific and the possible contribution of this difference to cellular oxidative stress. Oxygen consumption rate, NOX activity and mRNA levels, and the activity of catalase (CAT), glutathione peroxidase (GPX), and superoxide dismutase (SOD), as well as the reactive protein thiol levels, were measured in the soleus (SOL), red gastrocnemius (RG), and white gastrocnemius (WG) muscles of rats. RG showed higher oxygen consumption flow than SOL and WG, while SOL had higher oxygen consumption than WG. SOL showed higher NOX activity, as well as NOX2 and NOX4 mRNA levels, antioxidant enzymatic activities, and reactive protein thiol contents when compared to WG and RG. NOX activity and NOX4 mRNA levels as well as antioxidant enzymatic activities were higher in RG than in WG. Physical exercise increased NOX activity in SOL and RG, specifically NOX2 mRNA levels in RG and NOX4 mRNA levels in SOL. In conclusion, we demonstrated that NOX activity and expression differ according to the skeletal muscle fiber type, as well as antioxidant defense.
authors.affiliation: Laboratório de Expressão Gênica, Instituto Superior de Ciências Biomédicas, Universidade Estadual do Ceará, Fortaleza, CE, Brazil.
authors.forename: Adriano César Carneiro
authors.initials: AC
authors.lastname: Loureiro
authors.affiliation: Laboratório de Radiobiologia Molecular, Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brazil.
authors.forename: Igor Coutinho
authors.initials: IC
authors.lastname: do Rêgo-Monteiro
authors.affiliation: Laboratório de Fisiologia Endócrina Doris Rosenthal, Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brazil.
authors.forename: Ruy A
authors.initials: RA
authors.lastname: Louzada
authors.affiliation: Laboratório de Radiobiologia Molecular, Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brazil.
authors.forename: Victor Hugo
authors.initials: VH
authors.lastname: Ortenzi
authors.affiliation: Laboratório de Expressão Gênica, Instituto Superior de Ciências Biomédicas, Universidade Estadual do Ceará, Fortaleza, CE, Brazil.
authors.forename: Angélica Ponte
authors.initials: AP
authors.lastname: de Aguiar
authors.affiliation: Laboratório de Expressão Gênica, Instituto Superior de Ciências Biomédicas, Universidade Estadual do Ceará, Fortaleza, CE, Brazil.
authors.forename: Ewerton Sousa
authors.initials: ES
authors.lastname: de Abreu
authors.affiliation: Laboratório de Fisiologia Endócrina Doris Rosenthal, Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brazil.
authors.forename: João Paulo Albuquerque
authors.initials: JP
authors.lastname: Cavalcanti-de-Albuquerque
authors.affiliation: Laboratório de Fisiologia Endócrina Doris Rosenthal, Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brazil.
authors.forename: Fabio
authors.initials: F
authors.lastname: Hecht
authors.affiliation: Laboratório de Expressão Gênica, Instituto Superior de Ciências Biomédicas, Universidade Estadual do Ceará, Fortaleza, CE, Brazil.
authors.forename: Ariclécio Cunha
authors.initials: AC
authors.lastname: de Oliveira
authors.affiliation: Laboratório de Expressão Gênica, Instituto Superior de Ciências Biomédicas, Universidade Estadual do Ceará, Fortaleza, CE, Brazil.
authors.forename: Vânia Marilande
authors.initials: VM
authors.lastname: Ceccatto
authors.affiliation: Laboratório de Radiobiologia Molecular, Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brazil.
authors.forename: Rodrigo S
authors.initials: RS
authors.lastname: Fortunato
authors.affiliation: Laboratório de Fisiologia Endócrina Doris Rosenthal, Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brazil.
authors.forename: Denise P
authors.identifier: 0000-0001-6933-6424
authors.initials: DP
authors.lastname: Carvalho
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D006019:Glycolysis; D008297:Male; D018485:Muscle Fibers, Skeletal / Q000201:enzymology*; D019255:NADPH Oxidases / Q000235:genetics / Q000378:metabolism*; D010084:Oxidation-Reduction; D010101:Oxygen Consumption; D010805:Physical Conditioning, Animal; D012333:RNA, Messenger / Q000378:metabolism; D017208:Rats, Wistar; D060888:Real-Time Polymerase Chain Reaction; D013438:Sulfhydryl Compounds / Q000378:metabolism
chemical_list: D000975:Antioxidants; D012333:RNA, Messenger; D013438:Sulfhydryl Compounds; D019255:NADPH Oxidases