title: Determination of 1,2/1,3-diglycerides in Sicilian extra-virgin olive oils by1H-NMR over a one-year storage period.
abstract: This study is aimed to monitor by1H NMR spectroscopy the effect of a 12-month storage period on the 1,2-diglycerides over 1,3-diglycerides ratio for five mono-cultivar 'extra virgin olive oils' (EVOO) (Arbequina, Arbosana, Cerasuola, Nocellara and FS17) and one blend of two different cultivars (Nocellara + Biancolilla) preserved in the dark and at room temperature. These quantifications, at 500 MHz, are readily extracted through a specific and original integration difference method. Albeit it was known that the isomerisation rate is affected by the free acidity, we here demonstrate that it also depends on the presence of specific macromolecules (lipases), indeed, different EVOO cultivars with similar free acidity, show different isomerisation rate. Our results are consistent with similar diglyceride monitoring performed on Greek and Spanish EVOOs by31P NMR.
authors.affiliation: a Department BIOMORF , University of Messina , Messina , Italy.
authors.forename: Andrea
authors.initials: A
authors.lastname: Salvo
authors.affiliation: a Department BIOMORF , University of Messina , Messina , Italy.
authors.forename: Archimede
authors.initials: A
authors.lastname: Rotondo
authors.affiliation: a Department BIOMORF , University of Messina , Messina , Italy.
authors.forename: Giovanna Loredana
authors.initials: GL
authors.lastname: La Torre
authors.affiliation: a Department BIOMORF , University of Messina , Messina , Italy.
authors.forename: Nicola
authors.initials: N
authors.lastname: Cicero
authors.affiliation: a Department BIOMORF , University of Messina , Messina , Italy.
authors.forename: Giacomo
authors.initials: G
authors.lastname: Dugo
mesh_terms: D004075:Diglycerides / Q000032:analysis*; D061353:Food Storage; D007536:Isomerism; D009682:Magnetic Resonance Spectroscopy; D031658:Olea / Q000737:chemistry*; D000069463:Olive Oil / Q000032:analysis*; D066244:Proton Magnetic Resonance Spectroscopy
chemical_list: C011439:1,2-diacylglycerol; D004075:Diglycerides; D000069463:Olive Oil
keywords: 1,2-diglyceride; 1,3-diglyceride; NMR spectroscopy; extra-virgin olive oil; shelf life