title: Characterization of the microbiota associated to Pecten maximus gonads using 454-pyrosequencing.
abstract: A next-generation sequencing (NGS) approach was used to study the microbiota associated to Pecten maximus broodstock, applying pyrosequencing of PCR-amplified V1-V4 16S rRNA gene regions. We analysed the resident bacterial communities in female and male scallop gonads before and after spawning. DNA samples were amplified and quality-filtered reads were assigned to family and genus taxonomic levels using the Ribosomal Database Project classifier. A total of 18,520 sequences were detected, belonging to 13 phyla, including Proteobacteria (55%), Bacteroidetes (11,7%), Firmicutes (3%), Actinobacteria (2%) and Spirochaetes (1,2%), and 110 genera. The major fraction of the sequences detected corresponded to Proteobacteria, Beta- and Gammaprotebacteria being the most abundant classes. The microbiota of P. maximus gonad harbour a wide diversity, however differences on male and female samples were observed, where female gonad samples show a larger number of genera and families. The dominant bacterial genera appeared to be Delftia, Acinetobacter, Hydrotalea, Aquabacterium, Bacillus, Sediminibacterium, Sphingomonas, and Pseudomonas that were present among the four analysed samples. This next generation sequencing technique, applied for the first time in P. maximus (great scallop) gonads was useful for the study of the bacterial communities in this mollusc, unravelling the great bacterial diversity in its microbiota. [Int Microbiol 19(2): 93-99(2016)].
authors.affiliation: Department of Microbiology and Parasitology, CIBUS, Universidade de Santiago de Compostela, Santiago de Compostela, Spain.
authors.forename: Aide
authors.initials: A
authors.lastname: Lasa
authors.affiliation: Department of Genomics and Health, Foundation FISABIO, High Research Center of Public Health, Valencia, Spain.
authors.forename: Alex
authors.initials: A
authors.lastname: Mira
authors.affiliation: Department of Genomics and Health, Foundation FISABIO, High Research Center of Public Health, Valencia, Spain.
authors.forename: Anny
authors.initials: A
authors.lastname: Camelo-Castillo
authors.affiliation: Department of Genomics and Health, Foundation FISABIO, High Research Center of Public Health, Valencia, Spain.
authors.forename: Pedro
authors.initials: P
authors.lastname: Belda-Ferre
authors.affiliation: Department of Microbiology and Parasitology, CIBUS, Universidade de Santiago de Compostela, Santiago de Compostela, Spain.
authors.forename: Jesús L
authors.initials: JL
authors.lastname: Romalde
mesh_terms: D000818:Animals; D001419:Bacteria / Q000145:classification*; D004269:DNA, Bacterial / Q000235:genetics; D005260:Female; D006066:Gonads / Q000382:microbiology*; D008297:Male; D064307:Microbiota; D049910:Pecten / Q000382:microbiology*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S
keywords: Pecten maximus; aquaculture; gonads microbiota; molluscs pathogens; next-generation sequencing (NGS)