title: Sensitive detection of trimethylamine based on dopant-assisted positive photoionization ion mobility spectrometry.
abstract: Biogenic amines are degradation products generated through enzymatic and microbial processes during food spoilage, which may pose a health hazard to consumers at elevated levels. Trimethylamine (TMA) is a good target for the detection of biogenic amines due to its volatility and fishy odor. In this study, we developed a stand-alone dopant-assisted positive photoionization ion mobility spectrometry (DAPP-IMS) for rapid and sensitive detection of TMA. Response of TMA was enhanced by the addition of dopants and characteristic product ions with reduced mobility 2.26cm2V-1s-1were formed. 2-Butaone was chosen as the dopant for better separation between reagent ion peak and TMA product ion peak as well as higher sensitivity and the limit of detections (LODs) for TMA standard sample was 1ppb. The potential application of DAAP-IMS was evaluated by the detection of TMA generated by oyster and shrimp during 4°C storage. Analysis of two kinds of seafood showed the same characteristic peak to TMA standard sample, and the intensity of TMA increased over the storage time. The results of this study testify to the potential of DAPP-IMS for qualitative and quantitative determination of TMA in real food samples.
authors.affiliation: School of Food Science and Technology, Dalian Polytechnic University, National Engineering Research Center of Seafood, Dalian, Liaoning 116034, PR China.
authors.forename: Shasha
authors.initials: S
authors.lastname: Cheng
authors.affiliation: School of Food Science and Technology, Dalian Polytechnic University, National Engineering Research Center of Seafood, Dalian, Liaoning 116034, PR China.
authors.forename: Haitao
authors.initials: H
authors.lastname: Li
authors.affiliation: Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, Liaoning 116023, PR China.
authors.forename: Dandan
authors.initials: D
authors.lastname: Jiang
authors.affiliation: Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, Liaoning 116023, PR China.
authors.forename: Chuang
authors.initials: C
authors.lastname: Chen
authors.affiliation: School of Food Science and Technology, Dalian Polytechnic University, National Engineering Research Center of Seafood, Dalian, Liaoning 116034, PR China.
authors.forename: Tan
authors.initials: T
authors.lastname: Zhang
authors.affiliation: School of Food Science and Technology, Dalian Polytechnic University, National Engineering Research Center of Seafood, Dalian, Liaoning 116034, PR China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Li
authors.affiliation: School of Food Science and Technology, Dalian Polytechnic University, National Engineering Research Center of Seafood, Dalian, Liaoning 116034, PR China.
authors.forename: Haitao
authors.initials: H
authors.lastname: Wang
authors.affiliation: College of Environment, Zhejiang University of Technology, Hangzhou, Zhejiang 310014, PR China.
authors.forename: Qinghua
authors.initials: Q
authors.lastname: Zhou
authors.affiliation: Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, Liaoning 116023, PR China. Electronic address: hli@dicp.ac.cn.
authors.forename: Haiyang
authors.initials: H
authors.lastname: Li
authors.affiliation: School of Food Science and Technology, Dalian Polytechnic University, National Engineering Research Center of Seafood, Dalian, Liaoning 116034, PR China. Electronic address: mqtan@dlpu.edu.cn.
authors.forename: Mingqian
authors.initials: M
authors.lastname: Tan
mesh_terms: D000818:Animals; D002074:Butanones / Q000737:chemistry*; D003080:Cold Temperature; D000075663:Ion Mobility Spectrometry / Q000379:methods*; D007477:Ions / Q000737:chemistry; D008744:Methylamines / Q000032:analysis*; D010125:Ostreidae / Q000737:chemistry; D033561:Penaeidae / Q000737:chemistry; D015203:Reproducibility of Results; D017747:Seafood / Q000032:analysis*; D014835:Volatilization
chemical_list: D002074:Butanones; D007477:Ions; D008744:Methylamines; C005222:methylethyl ketone; C023336:trimethylamine
keywords: Biogenic amines; Dopant-assisted positive photoionization (DAPP); Ion mobility spectrometry (IMS); Oyster; Shrimp; Trimethylamine (TMA)