title: Copper is a Cofactor of the Formylglycine-Generating Enzyme.
abstract: Formylglycine-generating enzyme (FGE) is an O2-utilizing oxidase that converts specific cysteine residues of client proteins to formylglycine. We show that CuIis an integral cofactor of this enzyme and binds with high affinity (KD=of 10-17m) to a pair of active-site cysteines. These findings establish FGE as a novel type of copper enzyme.
authors.affiliation: Department of Chemistry, University of Basel, St. Johanns-Ring 19, 4056, Basel, Switzerland.
authors.forename: Matthias
authors.initials: M
authors.lastname: Knop
authors.affiliation: Department of Chemistry, University of Basel, St. Johanns-Ring 19, 4056, Basel, Switzerland.
authors.forename: Thanh Quy
authors.initials: TQ
authors.lastname: Dang
authors.affiliation: Laboratory of Physical Chemistry, ETH Zürich, Vladimir-Prelog-Weg 2, 8093, Zürich, Switzerland.
authors.forename: Gunnar
authors.initials: G
authors.lastname: Jeschke
authors.affiliation: Department of Chemistry, University of Basel, St. Johanns-Ring 19, 4056, Basel, Switzerland.
authors.forename: Florian P
authors.identifier: http://orcid.org/0000-0003-4625-1369
authors.initials: FP
authors.lastname: Seebeck
mesh_terms: D039903:Actinobacteria / Q000201:enzymology; D055162:Biocatalysis; D020134:Catalytic Domain; D003300:Copper / Q000737:chemistry / Q000378:metabolism*; D003545:Cysteine / Q000737:chemistry / Q000378:metabolism; D004578:Electron Spin Resonance Spectroscopy; D005998:Glycine / Q000031:analogs & derivatives* / Q000737:chemistry / Q000378:metabolism; D006801:Humans; D007700:Kinetics; D056004:Molecular Dynamics Simulation; D013429:Sulfatases / Q000737:chemistry / Q000378:metabolism*
chemical_list: C032736:N-formylglycine; D003300:Copper; D013429:Sulfatases; D003545:Cysteine; D005998:Glycine
keywords: EPR; copper; formylglycine; oxidases; oxygen activation; protein engineering