title: Human adipogenesis is associated with genome-wide DNA methylation and gene-expression changes.
abstract: To define the genomic distribution and function of DNA methylation changes during human adipogenesis.
  
  
  
  We isolated adipocyte-derived stem cells from 13 individuals and analyzed genome-wide DNA methylation and gene expression in cultured adipocyte-derived stem cells and mature adipocytes.
  
  
  
  We observed altered DNA methylation of 11,947 CpG sites and altered expression of 11,830 transcripts after differentiation. De novo methylation was observed across all genomic elements. Co-existence of genes with both altered expression and DNA methylation was found in genes important for cell cycle and adipokine signaling.
  
  
  
  Human adipogenesis is associated with significant DNA methylation changes across the entire genome and may impact regulation of cell cycle and adipokine signaling.
authors.affiliation: Department of Endocrinology, Diabetes & Metabolism, Rigshospitalet, Copenhagen, Denmark.
authors.forename: Christa
authors.initials: C
authors.lastname: Broholm
authors.affiliation: Department of Endocrinology, Diabetes & Metabolism, Rigshospitalet, Copenhagen, Denmark.
authors.forename: Anders Henrik
authors.initials: AH
authors.lastname: Olsson
authors.affiliation: Department of Clinical Sciences, Epigenetics & Diabetes Unit, Lund University Diabetes Centre, CRC, Malmö, Sweden.
authors.forename: Alexander
authors.initials: A
authors.lastname: Perfilyev
authors.affiliation: Department of Endocrinology, Diabetes & Metabolism, Rigshospitalet, Copenhagen, Denmark.
authors.forename: Linn
authors.initials: L
authors.lastname: Gillberg
authors.affiliation: Department of Endocrinology, Diabetes & Metabolism, Rigshospitalet, Copenhagen, Denmark.|Faculty of Health & Medical Sciences, University of Copenhagen, Copenhagen, Denmark.
authors.forename: Ninna Schiøler
authors.initials: NS
authors.lastname: Hansen
authors.affiliation: Department of Clinical Sciences, GAME, Lund University Diabetes Centre, CRC, Malmö, Sweden.
authors.forename: Ashfaq
authors.initials: A
authors.lastname: Ali
authors.affiliation: Steno Diabetes Centre A/S, Gentofte, Denmark.
authors.forename: Brynjulf
authors.initials: B
authors.lastname: Mortensen
authors.affiliation: Department of Clinical Sciences, Epigenetics & Diabetes Unit, Lund University Diabetes Centre, CRC, Malmö, Sweden.
authors.forename: Charlotte
authors.initials: C
authors.lastname: Ling
authors.affiliation: Department of Endocrinology, Diabetes & Metabolism, Rigshospitalet, Copenhagen, Denmark.|Faculty of Health & Medical Sciences, University of Copenhagen, Copenhagen, Denmark.|Steno Diabetes Centre A/S, Gentofte, Denmark.
authors.forename: Allan
authors.initials: A
authors.lastname: Vaag
mesh_terms: D017667:Adipocytes / Q000166:cytology; D050156:Adipogenesis / Q000235:genetics*; D052242:Adiponectin / Q000235:genetics; D000328:Adult; D002454:Cell Differentiation; D002478:Cells, Cultured; D018899:CpG Islands; D019175:DNA Methylation; D015870:Gene Expression; D015894:Genome, Human; D006801:Humans; D008297:Male; D011401:Promoter Regions, Genetic; D013234:Stem Cells / Q000378:metabolism; D055815:Young Adult
chemical_list: C499918:ADIPOQ protein, human; D052242:Adiponectin
keywords: DNA methylation; adipogenesis; adiponectin; epigenetics; stem cells