title: De novo transcriptome analysis of the red seaweed Gracilaria chilensis and identification of linkers associated with phycobilisomes.
abstract: This work reports the results of the Illumina RNA-Seq of a wild population of female haploid plants of Gracilaria chilensis (Bird et al., 1986) (Rhodophyta, Gigartinalis). Most transcripts were de novo assembled in 12,331 contigs with an average length of 1756bp, showing that 96.64% of the sequences were annotated with known proteins. In particular, the identification of linker proteins of phycobilisomes (PBS) is reported. Linker proteins have primary been identified in cyanobacteria but the information available about them in eukaryotic red alga is not complete, and this is the first report in G. chilensis. This resource will also provide the basis for the study of metabolic pathways related to polysaccharide production.
authors.affiliation: Laboratorio de Biofísica Molecular, Departamento de Bioquímica y Biología Molecular, Facultad de Ciencias Biológicas, Universidad de Concepción, Chile.
authors.forename: María Alejandra
authors.initials: MA
authors.lastname: Vorphal
authors.affiliation: Laboratory of Biotechnology and Aquatic Genomics, Interdisciplinary Center for Sustainable Aquaculture Research (INCAR), Department of Oceanography, Biotechnology Center, University of Concepción, Concepción, Chile. Electronic address: crisgallardo@udec.cl.
authors.forename: Cristian
authors.initials: C
authors.lastname: Gallardo-Escárate
authors.affiliation: Laboratory of Biotechnology and Aquatic Genomics, Interdisciplinary Center for Sustainable Aquaculture Research (INCAR), Department of Oceanography, Biotechnology Center, University of Concepción, Concepción, Chile.
authors.forename: Valentina
authors.initials: V
authors.lastname: Valenzuela-Muñoz
authors.affiliation: Laboratorio de Biofísica Molecular, Departamento de Bioquímica y Biología Molecular, Facultad de Ciencias Biológicas, Universidad de Concepción, Chile.
authors.forename: Jorge
authors.initials: J
authors.lastname: Dagnino-Leone
authors.affiliation: Laboratorio de Biofísica Molecular, Departamento de Bioquímica y Biología Molecular, Facultad de Ciencias Biológicas, Universidad de Concepción, Chile.
authors.forename: José Aleikar
authors.initials: JA
authors.lastname: Vásquez
authors.affiliation: Laboratorio de Biofísica Molecular, Departamento de Bioquímica y Biología Molecular, Facultad de Ciencias Biológicas, Universidad de Concepción, Chile.
authors.forename: José
authors.initials: J
authors.lastname: Martínez-Oyanedel
authors.affiliation: Laboratorio de Biofísica Molecular, Departamento de Bioquímica y Biología Molecular, Facultad de Ciencias Biológicas, Universidad de Concepción, Chile.
authors.forename: Marta
authors.initials: M
authors.lastname: Bunster
mesh_terms: D020418:Algal Proteins / Q000378:metabolism*; D002677:Chile; D020869:Gene Expression Profiling; D044724:Gracilaria / Q000378:metabolism*; D053858:Metabolic Networks and Pathways; D045524:Phycobilisomes / Q000378:metabolism*; D011134:Polysaccharides / Q000378:metabolism*; D012624:Seaweed / Q000378:metabolism; D059467:Transcriptome
chemical_list: D020418:Algal Proteins; D045524:Phycobilisomes; D011134:Polysaccharides
keywords: Gracilaria chilensis; Phycobilisomes; Rhodophyta; Transcriptome