title: Bupleurum chinense polysaccharide inhibit adhesion of human melanoma cells via blocking β1 integrin function.
abstract: Adhesive interaction contributes toward tumor metastasis and the transmembrane glycoprotein receptor, integrin has been recognized to mediate the adhesion to extracellular matrix thus upregulating tumor metastasis. In the current study, we evaluated the anti-adhesive mechanisms of a water-soluble polysaccharide (BCP) extracted from Bupleurum chinense. BCP inhibited integrin-mediated adhesion of human melanoma A375 cells to fibronectin but had no effects on nonspecific adhesion to poly-l-lysine. BCP also reduced β1 integrin ligand affinity for GST-FNIII9-10 proteins. The adhesion-dependent formation of F-actin stress fiber and focal adhesion (FA) was also inhibited by BCP treatment. The inhibition of BCP on integrin-mediated signaling is probably through inhibiting phosphorylation of focal adhesion kinase (FAK) and paxillin. Collectively, our current findings indicated that BCP may be a potential therapy for melanoma metastasis due to its inhibitory effects on integrin function.
authors.affiliation: Jilin Provincial Key Laboratory of Molecular Geriatric Medicine, Life Science Research Center, Beihua University, Jilin 132013, China; Wood Material Science and Engineering Key Laboratory of Jilin Province, Beihua University, Jilin 132013, China.
authors.forename: Haibin
authors.initials: H
authors.lastname: Tong
authors.affiliation: Wood Material Science and Engineering Key Laboratory of Jilin Province, Beihua University, Jilin 132013, China.
authors.forename: Guiquan
authors.initials: G
authors.lastname: Jiang
authors.affiliation: Division of Biomedical Sciences, Faculty of Medicine, Memorial University of Newfoundland, St. John's, NL, Canada.
authors.forename: Dake
authors.initials: D
authors.lastname: Qi
authors.affiliation: Research Center for Clinical & Translational Medicine, 302 Hospital, Beijing 100039, China.
authors.forename: Jingfeng
authors.initials: J
authors.lastname: Bi
authors.affiliation: Jilin Provincial Key Laboratory of Molecular Geriatric Medicine, Life Science Research Center, Beihua University, Jilin 132013, China.
authors.forename: Dan
authors.initials: D
authors.lastname: Tian
authors.affiliation: Jilin Provincial Key Laboratory of Molecular Geriatric Medicine, Life Science Research Center, Beihua University, Jilin 132013, China.
authors.forename: Xingang
authors.initials: X
authors.lastname: Guan
authors.affiliation: School of Chemical Engineering, Northeast Dianli University, Jilin 132012, China.
authors.forename: Sheng
authors.initials: S
authors.lastname: Zheng
authors.affiliation: Jilin Provincial Key Laboratory of Molecular Geriatric Medicine, Life Science Research Center, Beihua University, Jilin 132013, China. Electronic address: sunxinbh@126.com.
authors.forename: Xin
authors.initials: X
authors.lastname: Sun
mesh_terms: D029972:Bupleurum / Q000737:chemistry*; D002448:Cell Adhesion / Q000187:drug effects*; D045744:Cell Line, Tumor; D051417:Focal Adhesion Kinase 1 / Q000037:antagonists & inhibitors; D006801:Humans; D019012:Integrin beta1 / Q000378:metabolism*; D008545:Melanoma; D010766:Phosphorylation / Q000187:drug effects; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology*; D011134:Polysaccharides / Q000494:pharmacology*; D011485:Protein Binding / Q000187:drug effects
chemical_list: D019012:Integrin beta1; D010936:Plant Extracts; D011134:Polysaccharides; D051417:Focal Adhesion Kinase 1
keywords: Bupleurum chinense; Fibronectin; Focal adhesion; Integrin; Polysaccharide