title: Development of a Novel Test for Simultaneous Bacterial Identification and Antibiotic Susceptibility.
abstract: Background. Elucidation of a pathogen's antimicrobial susceptibility requires subculture after the organism is first isolated. This takes several days, requiring patients to be treated with broad-spectrum antibiotics. This approach contributes to the development of bacterial resistance.Methods. Microtiter wells were coated with a polyclonal antibody targeting the pathogen of interest. Bacterial suspensions were added in the presence/absence of selected antibiotics. After washing, captured bacteria were detected.Findings. Group B streptococcus (GBS),Enterococcus faecalis, andNeisseria gonorrhoeaewere each detected at 105bacteria/mL following a 20-minute incubation period. Susceptibility to select antibiotics was discernable following a 6-hour incubation period (GBS andEnterococcus). Sensitivity was increased to 10-2bacteria/mL for GBS, 10-1bacteria/mL forE. faecalis, and 101bacteria/mL forN. gonorrhoeaefollowing 18-24-hour culture.Conclusion. This novel assay allows for the highly sensitive and specific identification of a pathogen and simultaneous determination of its antimicrobial susceptibility in a reduced time.
authors.affiliation: The Woman's Hospital of Texas, 7400 Fannin Suite 930, Houston, TX 77054, USA.
authors.forename: Jonathan
authors.identifier: 0000-0002-7201-2399
authors.initials: J
authors.lastname: Faro
authors.affiliation: The University of Texas Health Science Center at Houston, Medical College, Houston, TX 77054, USA.
authors.forename: Malika
authors.initials: M
authors.lastname: Mitchell
authors.affiliation: The University of Texas Health Science Center at Houston, Medical College, Houston, TX 77054, USA.
authors.forename: Yuh-Jue
authors.initials: YJ
authors.lastname: Chen
authors.affiliation: The University of Texas Health Science Center at Houston, Medical College, Houston, TX 77054, USA.
authors.forename: Sarah
authors.initials: S
authors.lastname: Kamal
authors.affiliation: The Woman's Hospital of Texas, 7400 Fannin Suite 930, Houston, TX 77054, USA.
authors.forename: Gerald
authors.identifier: 0000-0002-6148-0234
authors.initials: G
authors.lastname: Riddle
authors.affiliation: The Woman's Hospital of Texas, 7400 Fannin Suite 930, Houston, TX 77054, USA.
authors.forename: Sebastian
authors.initials: S
authors.lastname: Faro
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001419:Bacteria / Q000187:drug effects / Q000235:genetics / Q000302:isolation & purification; D001424:Bacterial Infections / Q000382:microbiology*; D006801:Humans; D057230:Limit of Detection; D008826:Microbial Sensitivity Tests / Q000379:methods*; D058889:Molecular Typing / Q000379:methods*
chemical_list: D000900:Anti-Bacterial Agents