title: Long Noncoding RNA EWSAT1 Promotes Osteosarcoma Cell Growth and Metastasis Through Suppression of MEG3 Expression.
abstract: Osteosarcoma (OS) is the most common primary bone tumor in children and adolescents. Long noncoding RNAs (lncRNAs) are a class of transcriptional products of the genome without protein-coding potential. Recently, lncRNA Ewing sarcoma-associated transcript 1 (EWSAT1) was functionally identified in Ewing sarcoma, a highly aggressive primary pediatric bone tumor. However, whether EWSAT1 plays a role in OS remains unclear. In the present study, gain- and loss-of-function assays demonstrated that EWSAT1 enhanced OS cell proliferation, migration, and invasion. Further mechanistic studies found that EWSAT1 positively regulated lncRNA MEG3 expression in the transcriptional level. Finally, we observed that EWSAT1 facilitates OS cell growth and metastasis through regulation of MEG3, suggesting that EWSAT1-MEG3 axis might be a promising target for OS treatment.
authors.affiliation: 1 Department of Orthopedics, Cangzhou Central Hospital , Hebei, China .
authors.forename: Lishan
authors.initials: L
authors.lastname: Sun
authors.affiliation: 1 Department of Orthopedics, Cangzhou Central Hospital , Hebei, China .
authors.forename: Cheng
authors.initials: C
authors.lastname: Yang
authors.affiliation: 2 Department of Ultrasound, Cangzhou Central Hospital , Hebei, China .
authors.forename: Juan
authors.initials: J
authors.lastname: Xu
authors.affiliation: 3 Department of Endocrinology, Cangzhou Central Hospital , Hebei, China .
authors.forename: Yanhong
authors.initials: Y
authors.lastname: Feng
authors.affiliation: 1 Department of Orthopedics, Cangzhou Central Hospital , Hebei, China .
authors.forename: Liguo
authors.initials: L
authors.lastname: Wang
authors.affiliation: 1 Department of Orthopedics, Cangzhou Central Hospital , Hebei, China .
authors.forename: Tao
authors.initials: T
authors.lastname: Cui
mesh_terms: D000293:Adolescent; D017209:Apoptosis / Q000235:genetics; D001859:Bone Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D002453:Cell Cycle / Q000235:genetics; D002465:Cell Movement; D049109:Cell Proliferation; D002648:Child; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D009361:Neoplasm Invasiveness; D012516:Osteosarcoma / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D062085:RNA, Long Noncoding / Q000235:genetics* / Q000378:metabolism; D015398:Signal Transduction; D014158:Transcription, Genetic; D014407:Tumor Cells, Cultured
chemical_list: C498907:MEG3 non-coding RNA, human; D062085:RNA, Long Noncoding
keywords: EWSAT1; MEG3; lncRNA; metastasis