title: Synovial fluid concentrations of cold-inducible RNA-binding protein are associated with severity in knee osteoarthritis.
abstract: BACKGROUND
  Cold-inducible RNA-binding protein (CIRP) is a pro-inflammatory cytokine. We determined whether serum or synovial fluid concentrations are associated with severity in osteoarthritis (OA).
  
  
  METHODS
  CIRP concentrations in serum and synovial fluid from 156 knee OA patients and serum from 156 controls were determined. The Kellgren and Lawrence (KL) grade, Lequesne index and Western Ontario McMaster University Osteoarthritis Index (WOMAC) pain score were used to assess radiographic, clinical severity and pain severity respectively. Their scores were dichotomized based on their median values.
  
  
  RESULTS
  OA patients had similar serum CIRP concentrations compared to controls. In OA patients, CIRP concentrations in synovial fluid were dramatically higher compared to, but not correlated with paired serum samples. CIRP concentrations in synovial fluid were significantly correlated with synovial fluid or serum C-reactive protein, tumor necrosis factor- alpha and interleukin-6 concentrations, KL grade, Lequesne index and WOMAC pain score. Synovial fluid CIRP concentrations were independently related to the KL grade>2, Lequesne index >13 and WOMAC pain score>12. Under receiver operating characteristic curves, Synovial fluid CIRP concentrations significantly predicted them.
  
  
  CONCLUSIONS
  Increased synovial fluid CIRP concentrations were closely associated with the severity, substantializing CIRP as a potential marker for synovial inflammation of OA.
authors.affiliation: Department of Anesthesia and Pain, The Hangzhou First People's Hospital, Nanjing Medical University, 261 Huansha Rd., Hangzhou 310006, China.
authors.forename: Liang
authors.initials: L
authors.lastname: Yu
authors.affiliation: Department of Anesthesia and Pain, The Hangzhou First People's Hospital, Nanjing Medical University, 261 Huansha Rd., Hangzhou 310006, China.
authors.forename: Qing-Hua
authors.initials: QH
authors.lastname: Li
authors.affiliation: Department of Anesthesia and Pain, The Hangzhou First People's Hospital, Nanjing Medical University, 261 Huansha Rd., Hangzhou 310006, China.
authors.forename: Fang
authors.initials: F
authors.lastname: Deng
authors.affiliation: Department of Anesthesia and Pain, The Hangzhou First People's Hospital, Nanjing Medical University, 261 Huansha Rd., Hangzhou 310006, China.
authors.forename: Zheng-Wei
authors.initials: ZW
authors.lastname: Yu
authors.affiliation: Department of Anesthesia and Pain, The Hangzhou First People's Hospital, Nanjing Medical University, 261 Huansha Rd., Hangzhou 310006, China.
authors.forename: Xian-Zhe
authors.initials: XZ
authors.lastname: Luo
authors.affiliation: Department of Anesthesia and Pain, The Hangzhou First People's Hospital, Nanjing Medical University, 261 Huansha Rd., Hangzhou 310006, China. Electronic address: chinadataman@163.com.
authors.forename: Jian-Liang
authors.initials: JL
authors.lastname: Sun
mesh_terms: D003430:Cross-Sectional Studies; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D020370:Osteoarthritis, Knee / Q000175:diagnosis / Q000378:metabolism*; D016601:RNA-Binding Proteins / Q000378:metabolism*; D013582:Synovial Fluid / Q000378:metabolism*
chemical_list: C491390:CIRBP protein, human; D016601:RNA-Binding Proteins
keywords: Cold-inducible RNA-binding protein; Inflammation; Osteoarthritis; Severity; Synovial fluid