title: Development of single-nucleotide polymorphism markers forBromus tectorum(Poaceae) from a partially sequenced transcriptome.
abstract: OBJECTIVE
  Bromus tectorum(Poaceae) is an annual grass species that is invasive in many areas of the world but most especially in the U.S. Intermountain West. Single-nucleotide polymorphism (SNP) markers were developed for use in investigating the geospatial and ecological diversity ofB. tectorumin the Intermountain West to better understand the mechanisms behind its successful invasion.
  
  
  RESULTS
  Normalized cDNA libraries from six diverseB. tectorumindividuals were pooled and sequenced using 454 sequencing. Ninety-five SNP assays were developed for use on 96.96 arrays with the Fluidigm EP1 genotyping platform. Verification of the 95 SNPs by genotyping 251 individuals from 12 populations is reported, along with amplification data from four relatedBromusspecies.
  
  
  CONCLUSIONS
  These SNP markers are polymorphic across populations ofB. tectorum, are optimized for high-throughput applications, and may be applicable to other, relatedBromusspecies.
authors.affiliation: Shrub Sciences Laboratory, United States Forest Service, Rocky Mountain Research Station, 735 North 500 East, Provo, Utah 84606 USA; Department of Plant and Wildlife Sciences, 4105 Life Sciences Building, Brigham Young University, Provo, Utah 84602 USA.
authors.forename: Keith R
authors.initials: KR
authors.lastname: Merrill
authors.affiliation: Department of Plant and Wildlife Sciences, 4105 Life Sciences Building, Brigham Young University, Provo, Utah 84602 USA.
authors.forename: Craig E
authors.initials: CE
authors.lastname: Coleman
authors.affiliation: Shrub Sciences Laboratory, United States Forest Service, Rocky Mountain Research Station, 735 North 500 East, Provo, Utah 84606 USA.
authors.forename: Susan E
authors.initials: SE
authors.lastname: Meyer
authors.affiliation: Department of Natural Resources and Environmental Science, University of Nevada, Reno, 1664 N. Virginia Street, MS 186, Reno, Nevada 89557 USA.
authors.forename: Elizabeth A
authors.initials: EA
authors.lastname: Leger
authors.affiliation: Department of Plant and Wildlife Sciences, 4105 Life Sciences Building, Brigham Young University, Provo, Utah 84602 USA.
authors.forename: Katherine A
authors.initials: KA
authors.lastname: Collins
keywords: Bromus tectorum; Poaceae; cheatgrass; invasive; single-nucleotide polymorphism (SNP) development