title: Identification of snake venom allergens by two-dimensional electrophoresis followed by immunoblotting.
abstract: This allergic reaction to snake venom was described to occur in patients after recurrent exposure through bites in amateur and professional snake handlers, which might be underestimated and contribute to fatal snakebites in victim, independently from the toxicity of the venom itself. Few allergens were identified from snake venoms by normal SDS-PAGE, which cannot separate the snake venom completely. In the present study, we identified nine potential allergens by two-dimensional (2D) electrophoresis followed by immunoblotting (named as allergenomics) in Protobothrops mucrosquamatus venom. By multidimensional liquid chromatography-ion trap mass spectrometry (MDLC-ESI-LTQ-MS/MS) analysis, six allergens showed sequence similarity to snake venom serine proteinases. Other allergens showed sequence similarity to snake venom metalloproteinase. These allergic reactions to snake venom allergens might contribute to fatal snakebites in victim, independently.
authors.affiliation: Allergy and Clinical Immunology Research Centre, The First Affiliated Hospital of Jinzhou Medical University, Jinzhou, Liaoning 121001, China; Research Division of Clinical Pharmacology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, 210029, China; Department of Clinical Laboratory, The Changzhou No 2 People's Hospital, Changzhou, China.
authors.forename: Yujing
authors.initials: Y
authors.lastname: Hu
authors.affiliation: Jiangsu Collaborative Innovation Center of Regional Modern Agriculture and Environment Protection, Jiangsu Key Laboratory for Eco-Agriculture Biotechnology around Hongze Lake, Huaiyin Normal University, Huai'an, 223001, China.
authors.forename: Liming
authors.initials: L
authors.lastname: Yang
authors.affiliation: Department of Urology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, 210029, China.
authors.forename: Haiwei
authors.initials: H
authors.lastname: Yang
authors.affiliation: Allergy and Clinical Immunology Research Centre, The First Affiliated Hospital of Jinzhou Medical University, Jinzhou, Liaoning 121001, China. Electronic address: shoahenghe@hotmail.com.
authors.forename: Shaoheng
authors.initials: S
authors.lastname: He
authors.affiliation: Research Division of Clinical Pharmacology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, 210029, China. Electronic address: weijifu@hotmail.com.
authors.forename: Ji-Fu
authors.initials: JF
authors.lastname: Wei
mesh_terms: D000485:Allergens / Q000737:chemistry / Q000302:isolation & purification*; D002853:Chromatography, Liquid; D003435:Crotalid Venoms / Q000737:chemistry* / Q000276:immunology; D015180:Electrophoresis, Gel, Two-Dimensional; D006801:Humans; D006967:Hypersensitivity; D015151:Immunoblotting; D053719:Tandem Mass Spectrometry
chemical_list: D000485:Allergens; D003435:Crotalid Venoms
keywords: Allergen; Allergenomics; Metalloproteinase; Serine proteinase; Snake venom