title: Quantitative determination of non-starch polysaccharides in foods using Gas Chromatography with flame ionization detection.
abstract: A gas chromatographic method was developed for the quantitative determination of the monosaccharides of the soluble, insoluble fractions and the total non-starch polysaccharides (NSPs) content in foods with fat contents of less than 5%. Sample preparation involved enzymatic removal of starch and acid hydrolysis of the NSP to their constituent sugars. The alditol acetate derivatives were analysed on a wide bore capillary column with detection by flame ionization. The method was accurate, with recovery of spiked samples between 93.6% and 102.7% for intra-day analysis and between 93.2% and 104.7% for inter-day analysis. Repeatability was excellent; RSD values from 0.1 to 4.4% and 0.2 to 5.7% were observed from intra-day analysis and inter-day analysis, respectively. The peaks for all neutral sugars were sharp and separation was at baseline resolution with no interfering or co-eluting peaks.
authors.affiliation: Food Science and Technology Group, School of Chemical Engineering, UNSW Australia, Sydney 2052, Australia.
authors.forename: Jun
authors.initials: J
authors.lastname: Ma
authors.affiliation: Bioanalytical Mass Spectrometry Facility, Mark Wainwright Analytical Center, UNSW Australia, Sydney 2052, Australia.
authors.forename: Lewis
authors.initials: L
authors.lastname: Adler
authors.affiliation: Food Science and Technology Group, School of Chemical Engineering, UNSW Australia, Sydney 2052, Australia.
authors.forename: George
authors.initials: G
authors.lastname: Srzednicki
authors.affiliation: Food Science and Technology Group, School of Chemical Engineering, UNSW Australia, Sydney 2052, Australia; ARC Training Centre for Advanced Technologies in Food Manufacture, School of Chemical Engineering, UNSW Australia, Sydney 2052, Australia. Electronic address: j.arcot@unsw.edu.au.
authors.forename: Jayashree
authors.initials: J
authors.lastname: Arcot
mesh_terms: D002849:Chromatography, Gas; D004040:Dietary Carbohydrates / Q000032:analysis*; D004043:Dietary Fiber / Q000032:analysis*; D005410:Flame Ionization; D005504:Food Analysis / Q000379:methods*; D006868:Hydrolysis; D057230:Limit of Detection; D011134:Polysaccharides / Q000032:analysis*; D015203:Reproducibility of Results; D013213:Starch / Q000737:chemistry
chemical_list: D004040:Dietary Carbohydrates; D004043:Dietary Fiber; D011134:Polysaccharides; D013213:Starch
keywords: Banana pseudostem; Gas Chromatography; Non-starch polysaccharides