title: Analysis of transcriptome in hickory (Carya cathayensis), and uncover the dynamics in the hormonal signaling pathway during graft process.
abstract: Hickory (Carya cathayensis), a woody plant with high nutritional and economic value, is widely planted in China. Due to its long juvenile phase, grafting is a useful technique for large-scale cultivation of hickory. To reveal the molecular mechanism during the graft process, we sequenced the transcriptomes of graft union in hickory.
  
  
  
  In our study, six RNA-seq libraries yielded a total of 83,676,860 clean short reads comprising 4.19 Gb of sequence data. A large number of differentially expressed genes (DEGs) at three time points during the graft process were identified. In detail, 777 DEGs in the 7 d vs 0 d (day after grafting) comparison were classified into 11 enriched Gene Ontology (GO) categories, and 262 DEGs in the 14 d vs 0 d comparison were classified into 15 enriched GO categories. Furthermore, an overview of the PPI network was constructed by these DEGs. In addition, 20 genes related to the auxin-and cytokinin-signaling pathways were identified, and some were validated by qRT-PCR analysis.
  
  
  
  Our comprehensive analysis provides basic information on the candidate genes and hormone signaling pathways involved in the graft process in hickory and other woody plants.
authors.affiliation: Nurturing Station for the State Key Laboratory of Subtropical Silviculture, Zhejiang A & F University, Linan, Hangzhou, 311300, People's Republic of China.
authors.forename: Lingling
authors.initials: L
authors.lastname: Qiu
authors.affiliation: Nurturing Station for the State Key Laboratory of Subtropical Silviculture, Zhejiang A & F University, Linan, Hangzhou, 311300, People's Republic of China.
authors.forename: Bo
authors.initials: B
authors.lastname: Jiang
authors.affiliation: Nurturing Station for the State Key Laboratory of Subtropical Silviculture, Zhejiang A & F University, Linan, Hangzhou, 311300, People's Republic of China.|Center for Cultivation of Subtropical Forest Resources (CCSFR), Zhejiang A & F University, Linan, Hangzhou, 311300, People's Republic of China.
authors.forename: Jia
authors.initials: J
authors.lastname: Fang
authors.affiliation: Nurturing Station for the State Key Laboratory of Subtropical Silviculture, Zhejiang A & F University, Linan, Hangzhou, 311300, People's Republic of China.|Center for Cultivation of Subtropical Forest Resources (CCSFR), Zhejiang A & F University, Linan, Hangzhou, 311300, People's Republic of China.
authors.forename: Yike
authors.initials: Y
authors.lastname: Shen
authors.affiliation: Nurturing Station for the State Key Laboratory of Subtropical Silviculture, Zhejiang A & F University, Linan, Hangzhou, 311300, People's Republic of China.|Center for Cultivation of Subtropical Forest Resources (CCSFR), Zhejiang A & F University, Linan, Hangzhou, 311300, People's Republic of China.
authors.forename: Zhongxiang
authors.initials: Z
authors.lastname: Fang
authors.affiliation: Nurturing Station for the State Key Laboratory of Subtropical Silviculture, Zhejiang A & F University, Linan, Hangzhou, 311300, People's Republic of China.|Center for Cultivation of Subtropical Forest Resources (CCSFR), Zhejiang A & F University, Linan, Hangzhou, 311300, People's Republic of China.
authors.forename: Saravana Kumar
authors.initials: SK
authors.lastname: Rm
authors.affiliation: Key Laboratory of Plant Nutrition and Fertilizer, Ministry of Agriculture, Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.
authors.forename: Keke
authors.initials: K
authors.lastname: Yi
authors.affiliation: College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou, 310036, China.
authors.forename: Chenjia
authors.initials: C
authors.lastname: Shen
authors.affiliation: Nurturing Station for the State Key Laboratory of Subtropical Silviculture, Zhejiang A & F University, Linan, Hangzhou, 311300, People's Republic of China. liangsie2000@163.com.|Center for Cultivation of Subtropical Forest Resources (CCSFR), Zhejiang A & F University, Linan, Hangzhou, 311300, People's Republic of China. liangsie2000@163.com.
authors.forename: Daoliang
authors.initials: D
authors.lastname: Yan
authors.affiliation: Nurturing Station for the State Key Laboratory of Subtropical Silviculture, Zhejiang A & F University, Linan, Hangzhou, 311300, People's Republic of China. bszheng@zafu.edu.cn.|Center for Cultivation of Subtropical Forest Resources (CCSFR), Zhejiang A & F University, Linan, Hangzhou, 311300, People's Republic of China. bszheng@zafu.edu.cn.
authors.forename: Bingsong
authors.initials: B
authors.lastname: Zheng
mesh_terms: D031325:Carya / Q000235:genetics* / Q000378:metabolism*; D019295:Computational Biology / Q000379:methods; D003583:Cytokinins / Q000378:metabolism; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D063990:Gene Ontology; D059014:High-Throughput Nucleotide Sequencing; D007210:Indoleacetic Acids / Q000378:metabolism; D058977:Molecular Sequence Annotation; D010937:Plant Growth Regulators / Q000378:metabolism*; D025941:Protein Interaction Mapping; D060066:Protein Interaction Maps; D015203:Reproducibility of Results; D015398:Signal Transduction; D059467:Transcriptome
chemical_list: D003583:Cytokinins; D007210:Indoleacetic Acids; D010937:Plant Growth Regulators
keywords: Auxin; Cytokinin; Graft; Hickory; Transcriptome