title: Assessment of Immune Responses to Fungal Infections: Identification and Characterization of Immune Cells in the Infected Tissue.
abstract: The immune system is important to protect the host from fungal infections. Diverse cell types belonging to the innate or adaptive branch of the immune system act in a tightly coordinated and tissue-specific manner. Experimental mouse models of fungal infections have proved essential for assessing the protective principles against different fungal pathogens. Besides pathological, histological, biochemical and molecular parameters, the analysis of phenotypic and functional aspects of immune cells in infected tissues is key for understanding the mechanisms of antifungal defense. In this chapter, we describe a method based on flow cytometry to assess innate and adaptive immune cells isolated from an in vivo context in a qualitative and quantitative manner.
authors.affiliation: Section of Immunology, Institute of Virology, University of Zürich, Winterthurerstrasse 266a, Zürich, CH-8057, Switzerland.
authors.forename: Florian
authors.initials: F
authors.lastname: Sparber
authors.affiliation: Section of Immunology, Institute of Virology, University of Zürich, Winterthurerstrasse 266a, Zürich, CH-8057, Switzerland. salome.leibundgut-landmann@uzh.ch.
authors.forename: Salomé
authors.initials: S
authors.lastname: LeibundGut-Landmann
mesh_terms: D056704:Adaptive Immunity; D000818:Animals; D002176:Candida albicans / Q000276:immunology*; D002177:Candidiasis / Q000276:immunology* / Q000382:microbiology; D002469:Cell Separation; D005434:Flow Cytometry; D007962:Leukocytes / Q000276:immunology; D008197:Lymph Node Excision; D008810:Mice, Inbred C57BL
keywords: Candida albicans; Flow cytometry; Host defense; Innate and adaptive immunity; Mouse models; Mucosal tissue