title: Colorimetric detection of hydrogen peroxide and lactate based on the etching of the carbon based Au-Ag bimetallic nanocomposite synthesized by carbon dots as the reductant and stabilizer.
abstract: In this report, carbon-based gold core silver shell Au-Ag bimetallic nanocomposite (Au-Ag/C NC) was synthesized using carbon dots (C-dots) as the reductant and stabilizer by a facile green sequential reduction approach. The structure and morphology of the nanocomposite are characterized by ultraviolet-visible spectroscopy (UV-Vis), Fourier transform infrared spectroscopy (FT-IR) and transmission electron microscopy (TEM). The as synthesized Au-Ag/C NC exhibits good optic response toward hydrogen peroxide (H2O2) without adding any other chromogenic agents. The characteristic surface plasmon resonance (SPR) absorbance peak of Au-Ag/C NC declined and red-shifted with the solution color changing from reddish orange to light pink when adding H2O2owing to the etching effect of H2O2towards Ag. Thus, a simple colorimetric and UV strategy for sensitive detection of H2O2is proposed. It provides the wide linear range for detection of H2O2from 0.8-90 μM and 90-500 μM, and the detection limit was as low as 0.3 μM (S/N = 3). In addition, this colorimetric strategy can also be applied to directly distinguish and detect of lactate by naked eye and UV-Vis. The linear range of colorimetric sensing towards lactate was 0.1-22 μM and 22-220 μM, which was successfully applied in the analysis of lactate in human serum.
authors.affiliation: Key Laboratory of Chemical Biology and Traditional Chinese Medicine Research (Ministry of Education), College of Chemistry and Chemical Engineering, Hunan Normal University, Changsha 410081, PR China.
authors.forename: Lin
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Chemical Biology and Traditional Chinese Medicine Research (Ministry of Education), College of Chemistry and Chemical Engineering, Hunan Normal University, Changsha 410081, PR China.
authors.forename: Wenli
authors.initials: W
authors.lastname: Hou
authors.affiliation: Key Laboratory of Chemical Biology and Traditional Chinese Medicine Research (Ministry of Education), College of Chemistry and Chemical Engineering, Hunan Normal University, Changsha 410081, PR China.
authors.forename: Qiujun
authors.initials: Q
authors.lastname: Lu
authors.affiliation: Key Laboratory of Chemical Biology and Traditional Chinese Medicine Research (Ministry of Education), College of Chemistry and Chemical Engineering, Hunan Normal University, Changsha 410081, PR China. Electronic address: liumeilingww@126.com.
authors.forename: Meiling
authors.initials: M
authors.lastname: Liu
authors.affiliation: Key Laboratory of Chemical Biology and Traditional Chinese Medicine Research (Ministry of Education), College of Chemistry and Chemical Engineering, Hunan Normal University, Changsha 410081, PR China.
authors.forename: Chao
authors.initials: C
authors.lastname: Chen
authors.affiliation: Key Laboratory of Chemical Biology and Traditional Chinese Medicine Research (Ministry of Education), College of Chemistry and Chemical Engineering, Hunan Normal University, Changsha 410081, PR China.
authors.forename: Youyu
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Chemical Biology and Traditional Chinese Medicine Research (Ministry of Education), College of Chemistry and Chemical Engineering, Hunan Normal University, Changsha 410081, PR China.
authors.forename: Shouzhuo
authors.initials: S
authors.lastname: Yao
mesh_terms: D002244:Carbon / Q000737:chemistry*; D060326:Chemistry Techniques, Synthetic; D003124:Colorimetry / Q000379:methods*; D006046:Gold / Q000737:chemistry*; D055772:Green Chemistry Technology; D006861:Hydrogen Peroxide / Q000032:analysis* / Q000737:chemistry; D019344:Lactic Acid / Q000032:analysis* / Q000737:chemistry; D053768:Metal Nanoparticles / Q000737:chemistry; D053761:Nanocomposites / Q000737:chemistry*; D045663:Quantum Dots / Q000737:chemistry; D019163:Reducing Agents / Q000737:chemistry; D012834:Silver / Q000737:chemistry*
chemical_list: D019163:Reducing Agents; D019344:Lactic Acid; D012834:Silver; D002244:Carbon; D006046:Gold; D006861:Hydrogen Peroxide
keywords: C-dots; Carbon-based Au-Ag bimetallic nanocomposite; H(2)O(2); Lactate