title: Ischemic Postconditioning Assessment in the Liver of Rats Undergoing Mesenteric Ischemia and Reperfusion.
abstract: Ischemic postconditioning is a method that shows evidence of efficacy in minimizing reperfusion injury; however, its effectiveness in preventing injuries in distant organs is still unknown, especially in those who have undergone mesenteric ischemia and reperfusion.
  
  
  
  To evaluate the effect of ischemic postconditioning in preventing reperfusion injury in the liver of rats submitted to mesenteric ischemia and reperfusion, comparing two different methods of ischemic postconditioning.
  
  
  
  30 Wistar male rats were used, distributed into three groups: Group A: Ten rats submitted to intestinal ischemia for 30 minutes followed by reperfusion for 60 minutes; Group B: Ten rats subjected to ischemia and reperfusion; after ischemia, two cycles of reperfusion (two minutes each) interleaved with two cycles of ischemia (two minutes each); and Group C: Ten rats subjected to ischemia and reperfusion; after ischemia, four cycles of reperfusion (30 seconds each) interspersed with four cycles of ischemia (30 seconds each). After the experiment, the left lobe of the liver was resected for subsequent histological analysis, using the following classification: grade 1 - centrilobular congestion; grade 2 - centrilobular congestion with some degeneration of hepatocytes in one or two central veins; and grade 3 - multifocal centrilobular congestion and degeneration of portal hepatocytes.
  
  
  
  The mean degree of liver damage found was 1.8 in group A, 1.7 in group B and 1.3 in group C. There was no statistically significant difference between the groups.
  
  
  
  Ischemic postconditioning was unable to minimize reperfusion injury in rats undergoing mesenteric ischemia and reperfusion.
authors.affiliation: Faculdade de Medicina da Universidade Federal de Mato Grosso do Sul, Campo Grande, MS, Brazil.
authors.forename: Carlos Henrique Marques
authors.initials: CHM
authors.lastname: dos Santos
authors.affiliation: Faculdade de Medicina da Universidade Federal de Mato Grosso do Sul, Campo Grande, MS, Brazil.
authors.forename: Ricardo Dutra
authors.initials: RD
authors.lastname: Aydos
authors.affiliation: Faculdade de Medicina da Universidade Federal de Mato Grosso do Sul, Campo Grande, MS, Brazil.
authors.forename: Ed
authors.initials: E
authors.lastname: Nogueira
authors.affiliation: Faculdade de Medicina da Universidade Federal de Mato Grosso do Sul, Campo Grande, MS, Brazil.
authors.forename: Luciana Nakao Odashiro
authors.initials: LN
authors.lastname: Miiji
authors.affiliation: Faculdade de Medicina da Universidade Federal de Mato Grosso do Sul, Campo Grande, MS, Brazil.
authors.forename: Pedro Carvalho
authors.initials: PC
authors.lastname: Cassino
authors.affiliation: Faculdade de Medicina da Universidade Federal de Mato Grosso do Sul, Campo Grande, MS, Brazil.
authors.forename: Isadora Ishaq
authors.initials: II
authors.lastname: Alves
authors.affiliation: Faculdade de Medicina da Universidade Federal de Mato Grosso do Sul, Campo Grande, MS, Brazil.
authors.forename: Nádia Meneguesso
authors.initials: NM
authors.lastname: Calheiros
authors.affiliation: Faculdade de Medicina da Universidade Federal de Mato Grosso do Sul, Campo Grande, MS, Brazil.
authors.forename: Milena
authors.initials: M
authors.lastname: Garcia
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D007413:Intestinal Mucosa / Q000098:blood supply / Q000473:pathology; D057775:Ischemic Postconditioning / Q000379:methods*; D008099:Liver / Q000098:blood supply / Q000473:pathology*; D008297:Male; D065666:Mesenteric Ischemia / Q000150:complications / Q000473:pathology*; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000150:complications / Q000473:pathology*