title: Biochemical characterization of PE_PGRS61 family protein ofMycobacterium tuberculosisH37Rv reveals the binding ability to fibronectin.
abstract: OBJECTIVE
  The periodic binding of protein expressed byMycobacterium tuberculosisH37Rv with the host cell receptor molecules i.e. fibronectin (Fn) is gaining significance because of its adhesive properties. The genome sequencing ofM. tuberculosisH37Rv revealed that the proline-glutamic (PE) proteins contain polymorphic GC-rich repetitive sequences (PGRS) which have clinical importance in pathogenesis events when the host encountersM. tuberculosisH37Rv. The functional parts of PE_PGRS family proteins, have not been extensively studied in tuberculosis biology.
  
  
  METHODS
  Fibronectin (10 ng and 20 ng) were used for FnBP assay and its enzymatic activities were observed by using various protein concentrations.
  
  
  RESULTS
  Therefore, in the present work, we cloned, expressed, purified and identified a novel PE_PGRS61 (Rv3653) family protein inM. tuberculosisH37Rv. Our experiment, observation suggested that at particular concentrations of 10 ng and 20 ng of Fn exhibits optimum binding to the purified Fibronectin Binding Protein (FnBP), a PE_PGRS61 family protein at 0.20 μg and 0.25 μg concentrations, respectively. Moreover, for better understanding the computational analysis, the B-cell and T-cell epitopes prediction prospect some amino acid propensity scales with hydrophilicity and antigenic variation index at their respective locations.
  
  
  CONCLUSIONS
  Thus, the current findings provide an opportunity to illuminate the functions of PE_PGRS61 family protein. So, in this point of view, it could be useful to develop a novel therapeutic approach or diagnostic pipeline through targeting these fibronectin binding protein (FnBP) expressing genes.
authors.affiliation: CSIR-Institute of Genomics and Integrative Biology, Council of Scientific and Industrial Research, Mall Road, Delhi-110007.
authors.lastname: Monu
authors.affiliation: CSIR-Institute of Genomics and Integrative Biology, Council of Scientific and Industrial Research, Mall Road, Delhi-110007.
authors.forename: Laxman S
authors.initials: LS
authors.lastname: Meena
keywords: Fibronectin; Fibronectin binding - protein; Macrophages; Mycobacterium – tuberculosis; PE_PGRS