title: Unanimous Model for Describing the Fast Bioluminescence Kinetics of Ca2+-regulated Photoproteins of Different Organisms.
abstract: Upon binding their metal ion cofactors, Ca2+-regulated photoproteins display a rapid increase of light signal, which reaches its peak within milliseconds. In the present study, we investigate bioluminescence kinetics of the entire photoprotein family. All five recombinant hydromedusan Ca2+-regulated photoproteins-aequorin from Aequorea victoria, clytin from Clytia gregaria, mitrocomin from Mitrocoma cellularia and obelins from Obelia longissima and Obelia geniculata-demonstrate the same bioluminescent kinetics pattern. Based on these findings, for the first time we propose a unanimous kinetic model describing the bioluminescence mechanism of Ca2+-regulated photoproteins.
authors.affiliation: Photobiology Laboratory, Institute of Biophysics SB RAS, Federal Research Center "Krasnoyarsk Science Center SB RAS", Krasnoyarsk, Russia.
authors.forename: Elena V
authors.initials: EV
authors.lastname: Eremeeva
authors.affiliation: Theoretical Biophysics Laboratory, Institute of Biophysics SB RAS, Federal Research Center "Krasnoyarsk Science Center SB RAS", Krasnoyarsk, Russia.
authors.forename: Sergey I
authors.initials: SI
authors.lastname: Bartsev
authors.affiliation: Laboratory of Biochemistry, Wageningen University & Research, Wageningen, The Netherlands.
authors.forename: Willem J H
authors.initials: WJ
authors.lastname: van Berkel
authors.affiliation: Photobiology Laboratory, Institute of Biophysics SB RAS, Federal Research Center "Krasnoyarsk Science Center SB RAS", Krasnoyarsk, Russia.
authors.forename: Eugene S
authors.initials: ES
authors.lastname: Vysotski
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism*; D037402:Hydrozoa / Q000145:classification / Q000378:metabolism*; D007700:Kinetics; D049449:Luminescence; D008164:Luminescent Proteins / Q000378:metabolism*; D008954:Models, Biological
chemical_list: D008164:Luminescent Proteins; D002118:Calcium