title: Oral exposure to diisodecyl phthalate aggravates allergic dermatitis by oxidative stress and enhancement of thymic stromal lymphopoietin.
abstract: Diisodecyl phthalate (DIDP) is extensively used as an environmentally friendly plasticizer. However, little is known about the adverse effects and the underlying mechanisms of DIDP exposure on immunological diseases. We aimed to determine the role and mechanisms of DIDP exposure in allergic contact dermatitis-like skin lesions. We show that oral DIDP exposure can aggravate allergic dermatitis in mice. Moreover, an increase of ROS, total serum IgE and IL-4 levels were concomitant with this deterioration. We detected the expression of Thymic stromal lymphopoietin (TSLP) and the activation of STATs and NF-κB signal pathways. The data indicated that DIDP in combination with FITC triggers TSLP production. Our results also suggested that DIDP exacerbated the activation of NF-κB signal pathways, with an enhancement in TSLP expression, which potentiated the activation of STATs and the degranulation of mast cells in the skin, and finally exacerbated allergic dermatitis. The study also suggested that melatonin enhanced the expression of Nrf2, up-regulated the antioxidant genes HO-1 and NQO1, reduced the levels of oxidative stress and TSLP, and alleviated allergic dermatitis. The results demonstrated that DIDP exacerbated allergic dermatitis through oxidative stress and enhanced TSLP production.
authors.affiliation: Hubei Key Laboratory of Genetic Regulation and Integrative Biology, School of Life Sciences, Central China Normal University, Wuhan 430079, Hubei, China.
authors.forename: Shiping
authors.initials: S
authors.lastname: Shen
authors.affiliation: Hubei Key Laboratory of Genetic Regulation and Integrative Biology, School of Life Sciences, Central China Normal University, Wuhan 430079, Hubei, China.
authors.forename: Jinquan
authors.initials: J
authors.lastname: Li
authors.affiliation: Hubei Key Laboratory of Genetic Regulation and Integrative Biology, School of Life Sciences, Central China Normal University, Wuhan 430079, Hubei, China.
authors.forename: Huihui
authors.initials: H
authors.lastname: You
authors.affiliation: Hubei Key Laboratory of Genetic Regulation and Integrative Biology, School of Life Sciences, Central China Normal University, Wuhan 430079, Hubei, China.
authors.forename: Zhuo
authors.initials: Z
authors.lastname: Wu
authors.affiliation: College of Basic Medical Sciences, Hubei University of Science and Technology, Xianning 437100, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Wu
authors.affiliation: Hubei Key Laboratory of Genetic Regulation and Integrative Biology, School of Life Sciences, Central China Normal University, Wuhan 430079, Hubei, China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: Hubei Key Laboratory of Genetic Regulation and Integrative Biology, School of Life Sciences, Central China Normal University, Wuhan 430079, Hubei, China.
authors.forename: Yuqing
authors.initials: Y
authors.lastname: Zhu
authors.affiliation: Hubei Key Laboratory of Genetic Regulation and Integrative Biology, School of Life Sciences, Central China Normal University, Wuhan 430079, Hubei, China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Guo
authors.affiliation: Hubei Key Laboratory of Genetic Regulation and Integrative Biology, School of Life Sciences, Central China Normal University, Wuhan 430079, Hubei, China.
authors.forename: Xiaoxiao
authors.initials: X
authors.lastname: Li
authors.affiliation: Hubei Key Laboratory of Genetic Regulation and Integrative Biology, School of Life Sciences, Central China Normal University, Wuhan 430079, Hubei, China.
authors.forename: Rui
authors.initials: R
authors.lastname: Li
authors.affiliation: College of Basic Medical Sciences, Hubei University of Science and Technology, Xianning 437100, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Ma
authors.affiliation: Hubei Key Laboratory of Genetic Regulation and Integrative Biology, School of Life Sciences, Central China Normal University, Wuhan 430079, Hubei, China. Electronic address: yangxu@mail.ccnu.edu.cn.
authors.forename: Xu
authors.initials: X
authors.lastname: Yang
authors.affiliation: Hubei Key Laboratory of Genetic Regulation and Integrative Biology, School of Life Sciences, Central China Normal University, Wuhan 430079, Hubei, China. Electronic address: chenmq@mail.ccnu.edu.cn.
authors.forename: Mingqing
authors.initials: M
authors.lastname: Chen
mesh_terms: D000284:Administration, Oral; D000818:Animals; D016207:Cytokines / Q000378:metabolism*; D017449:Dermatitis, Allergic Contact / Q000209:etiology* / Q000378:metabolism / Q000473:pathology; D007124:Immunoenzyme Techniques; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D016328:NF-kappa B / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects*; D010795:Phthalic Acids / Q000008:administration & dosage / Q000633:toxicity*; D010968:Plasticizers / Q000008:administration & dosage / Q000633:toxicity; D017382:Reactive Oxygen Species / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects; D000094632:Thymic Stromal Lymphopoietin
chemical_list: D016207:Cytokines; D016328:NF-kappa B; D010795:Phthalic Acids; D010968:Plasticizers; D017382:Reactive Oxygen Species; C042080:diisodecyl phthalate; D000094632:Thymic Stromal Lymphopoietin; C000721967:TSLP protein, mouse
keywords: Allergic contact dermatitis; Diisodecyl phthalate (DIDP); NF-κB; Oxidative stress; Thymic stromal lymphopoietin (TSLP)