title: Analysis of enteroendocrine cell populations in the human colon.
abstract: Recent studies have shown that patterns of colocalisation of hormones in enteroendocrine cells are more complex than previously appreciated and that the patterns differ substantially between species. In this study, the human sigmoid colon is investigated by immunohistochemistry for the presence of gastrointestinal hormones and their colocalisation. The segments of colon were distant from the pathology that led to colectomy and appeared structurally normal. Only four hormones, 5-hydroxytryptamine (5-HT), glucagon-like peptide 1 (GLP-1), peptide YY (PYY) and somatostatin, were common in enteroendocrine cells of the human colon. Cholecystokinin, present in the colon of some species, was absent, as were glucose-dependent insulinotropic peptide, ghrelin and motilin. Neurotensin cells were extremely rare. The most numerous cells were 5-HT cells, some of which also contained PYY or somatostatin and very rarely GLP-1. Almost all GLP-1 cells contained PYY. It is concluded that enteroendocrine cells of the human colon, like those of other regions and species, exhibit overlapping patterns of hormone colocalisation and that the hormones and their patterns of expression differ between human and other species.
authors.affiliation: Department of Anatomy & Neuroscience, University of Melbourne, Parkville, Victoria, 3010, Australia.|Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil.
authors.forename: Patricia
authors.initials: P
authors.lastname: Martins
authors.affiliation: Department of Anatomy & Neuroscience, University of Melbourne, Parkville, Victoria, 3010, Australia.
authors.forename: Josiane
authors.initials: J
authors.lastname: Fakhry
authors.affiliation: Universidade Federal de Goiás, Goiânia - GO, Brazil.
authors.forename: Enio Chaves
authors.initials: EC
authors.lastname: de Oliveira
authors.affiliation: Department of Anatomy & Neuroscience, University of Melbourne, Parkville, Victoria, 3010, Australia.
authors.forename: Billie
authors.initials: B
authors.lastname: Hunne
authors.affiliation: Department of Anatomy & Neuroscience, University of Melbourne, Parkville, Victoria, 3010, Australia.
authors.forename: Linda J
authors.initials: LJ
authors.lastname: Fothergill
authors.affiliation: Department of Anatomy & Neuroscience, University of Melbourne, Parkville, Victoria, 3010, Australia.
authors.forename: Mitchell
authors.initials: M
authors.lastname: Ringuet
authors.affiliation: Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil.
authors.forename: Débora d'Ávila
authors.initials: DD
authors.lastname: Reis
authors.affiliation: Department of Clinical Biochemistry, Rigshospitalet, University of Copenhagen, Copenhagen, Denmark.
authors.forename: Jens F
authors.initials: JF
authors.lastname: Rehfeld
authors.affiliation: Department of Anatomy & Neuroscience, University of Melbourne, Parkville, Victoria, 3010, Australia.
authors.forename: Brid
authors.initials: B
authors.lastname: Callaghan
authors.affiliation: Department of Anatomy & Neuroscience, University of Melbourne, Parkville, Victoria, 3010, Australia. j.furness@unimelb.edu.au.|Florey Institute of Neuroscience and Mental Health, Parkville, Victoria, 3010, Australia. j.furness@unimelb.edu.au.
authors.forename: John B
authors.initials: JB
authors.lastname: Furness
mesh_terms: D002452:Cell Count; D003106:Colon / Q000166:cytology*; D019858:Enteroendocrine Cells / Q000166:cytology*; D006728:Hormones / Q000378:metabolism; D006801:Humans; D007583:Jejunum / Q000166:cytology; D013194:Staining and Labeling
chemical_list: D006728:Hormones
keywords: Enteroendocrine cells; Gastrointestinal hormones; Human chemical coding