title: Liquid Chromatography-Tandem Mass Spectrometry: An Emerging Technology in the Toxicology Laboratory.
abstract: In the last decade, liquid chromatography-tandem mass spectrometry (LC-MS/MS) has seen enormous growth in routine toxicology laboratories. LC-MS/MS offers significant advantages over other traditional testing, such as immunoassay and gas chromatography-mass spectrometry methodologies. Major strengths of LC-MS/MS include improvement in specificity, flexibility, and sample throughput when compared with other technologies. Here, the basic principles of LC-MS/MS technology are reviewed, followed by advantages and disadvantages of this technology compared with other traditional techniques. In addition, toxicology applications of LC-MS/MS for simultaneous detection of large panels of analytes are presented.
authors.affiliation: University of Rochester Medical Center, 601 Elmwood Avenue, Box 608, Rochester, NY 14642, USA.
authors.forename: Yan Victoria
authors.initials: YV
authors.lastname: Zhang
authors.affiliation: Toxicology Laboratory, Michigan Surgical Hospital, 21230 Dequindre Road, Warren, MI 48091, USA.
authors.forename: Bin
authors.initials: B
authors.lastname: Wei
authors.affiliation: Consolidated Laboratory Management Systems, LLC, 24555 Southfield Road, Southfield, MI 48075, USA. Electronic address: yu.zhu@consolidatedlabsmgt.com.
authors.forename: Yu
authors.initials: Y
authors.lastname: Zhu
authors.affiliation: Total Toxicology Labs, LLC, 24525 Southfield Road Suite 100, Southfield, MI 48075, USA.
authors.forename: Yanhua
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Consolidated Laboratory Management Systems, LLC, 24555 Southfield Road, Southfield, MI 48075, USA; Department of Pathology, Wayne State University School of Medicine, 540 East Canfield, Detroit, MI 48201, USA.
authors.forename: Martin H
authors.initials: MH
authors.lastname: Bluth
mesh_terms: D002853:Chromatography, Liquid / Q000379:methods / Q000639:trends*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods / Q000639:trends; D007753:Laboratories; D012680:Sensitivity and Specificity; D053719:Tandem Mass Spectrometry / Q000379:methods / Q000639:trends*
keywords: APCI; APPI; ESI; Immunoassay; LC-MS/MS; MRM; Quadrupole mass spectrometer; Toxicology application