title: Synthesis and facile end-group quantification of functionalized PEG azides.
abstract: Azido-functionalized poly(ethylene glycol) (PEG) derivatives are finding ever-increasing applications in the areas of conjugation chemistry and targeted drug delivery by their judicious incorporation into nanoparticle-forming polymeric systems. Quantification of azide incorporation into such PEG polymers is essential to their effective use.1H Nuclear Magnetic Resonance (NMR) analysis offers the simplest approach; however, the relevant adjacent azide-bearing methylene protons are often obscured by the PEG manifold signals. This study describes the synthesis of 1,2,3-triazole adducts from their corresponding PEG azides via a convenient, mild click reaction, which facilitates straightforward NMR-based quantitative end-group analysis.This method was found to be compatible with many examples of bifunctional azido PEGs with molecular weights ranging from 2 to 18 kDa bearing a variety of functional groups. © 2016 The Authors. Journal of Polymer Science Part A: Polymer Chemistry Published by Wiley Periodicals, Inc. J. Polym. Sci., Part B: Polym. Phys.2016,54, 2888-2895.
authors.affiliation: Intezyne Technologies 3720 Spectrum Blvd, Suite 104 Tampa Florida 33612.
authors.forename: J
authors.initials: J
authors.lastname: Edward Semple
authors.affiliation: Intezyne Technologies 3720 Spectrum Blvd, Suite 104 Tampa Florida 33612.
authors.forename: Bradford
authors.initials: B
authors.lastname: Sullivan
authors.affiliation: Intezyne Technologies 3720 Spectrum Blvd, Suite 104 Tampa Florida 33612.
authors.forename: Tomas
authors.initials: T
authors.lastname: Vojkovsky
authors.affiliation: Intezyne Technologies 3720 Spectrum Blvd, Suite 104 Tampa Florida 33612.
authors.forename: Kevin N
authors.initials: KN
authors.lastname: Sill
keywords: NMR spectroscopy; azides; calculations; click chemistry; hydrophilic polymers; poly(ethylene glycol); triazoles; water‐soluble polymers