title: Expression of Steroid Receptors in Ameloblasts during Amelogenesis in Rat Incisors.
abstract: Endocrine disrupting chemicals (EDCs) play a part in the modern burst of diseases and interfere with the steroid hormone axis. Bisphenol A (BPA), one of the most active and widely used EDCs, affects ameloblast functions, leading to an enamel hypomineralization pattern similar to that of Molar Incisor Hypomineralization (MIH). In order to explore the molecular pathways stimulated by BPA during amelogenesis, we thoroughly investigated the receptors known to directly or indirectly mediate the effects of BPA. The expression patterns of high affinity BPA receptors (ERRγ, GPR30), of ketosteroid receptors (ERs, AR, PGR, GR, MR), of the retinoid receptor RXRα, and PPARγ were established using RT-qPCR analysis of RNAs extracted from microdissected enamel organ of adult rats. Their expression was dependent on the stage of ameloblast differentiation, except that of ERβ and PPARγ which remained undetectable. An additional large scale microarray analysis revealed three main groups of receptors according to their level of expression in maturation-stage ameloblasts. The expression level of RXRα was the highest, similar to the vitamin D receptor (VDR), whereas the others were 13 to 612-fold lower, with AR and GR being intermediate. Immunofluorescent analysis of VDR, ERα and AR confirmed their presence mainly in maturation- stage ameloblasts. These data provide further evidence that ameloblasts express a specific combination of hormonal receptors depending on their developmental stage. This study represents the first step toward understanding dental endocrinology as well as some of the effects of EDCs on the pathophysiology of amelogenesis.
authors.affiliation: Paris Laboratory of Molecular Oral Pathophysiology, Centre de Recherche des Cordeliers, Institut National de la Santé et de la Recherche Médicale UMRS 1138, Université Paris-Descartes, Université Pierre et Marie Curie-ParisParis, France; Université Paris-Diderot, Unité de Formation et de Recherche d'OdontologieParis, France.
authors.forename: Sophia
authors.initials: S
authors.lastname: Houari
authors.affiliation: Paris Laboratory of Molecular Oral Pathophysiology, Centre de Recherche des Cordeliers, Institut National de la Santé et de la Recherche Médicale UMRS 1138, Université Paris-Descartes, Université Pierre et Marie Curie-ParisParis, France; Université Paris-Diderot, Unité de Formation et de Recherche d'OdontologieParis, France.
authors.forename: Sophia
authors.initials: S
authors.lastname: Loiodice
authors.affiliation: Paris Laboratory of Molecular Oral Pathophysiology, Centre de Recherche des Cordeliers, Institut National de la Santé et de la Recherche Médicale UMRS 1138, Université Paris-Descartes, Université Pierre et Marie Curie-ParisParis, France; Université Paris-Diderot, Unité de Formation et de Recherche d'OdontologieParis, France.
authors.forename: Katia
authors.initials: K
authors.lastname: Jedeon
authors.affiliation: Paris Laboratory of Molecular Oral Pathophysiology, Centre de Recherche des Cordeliers, Institut National de la Santé et de la Recherche Médicale UMRS 1138, Université Paris-Descartes, Université Pierre et Marie Curie-ParisParis, France; Université Paris-Diderot, Unité de Formation et de Recherche d'OdontologieParis, France; Centre de Référence des maladies rares de la face et de la cavité buccale MAFACE hôpital Rothschild, AP-HPParis, France.
authors.forename: Ariane
authors.initials: A
authors.lastname: Berdal
authors.affiliation: Paris Laboratory of Molecular Oral Pathophysiology, Centre de Recherche des Cordeliers, Institut National de la Santé et de la Recherche Médicale UMRS 1138, Université Paris-Descartes, Université Pierre et Marie Curie-ParisParis, France; Université Paris-Diderot, Unité de Formation et de Recherche d'OdontologieParis, France.
authors.forename: Sylvie
authors.initials: S
authors.lastname: Babajko
keywords: amelogenesis; enamel mineralization; endocrine disrupting chemicals; steroid hormones; steroid receptors