title: Highly sensitive and selective miRNA detection based on a closed ring probe and multiple signal amplification.
abstract: A highly sensitive and selective miRNA detection approach based on a small circular probe and dual signal amplification has been reported. We successfully applied the assay for quantitative let-7a detection in cell lysates and the results agreed well with those of qRT-PCR.
authors.affiliation: School of Pharmaceutical Sciences and Innovative Drug Research Center, Chongqing University, Chongqing, 401331, China. fengxuli@cqu.edu.cn.
authors.forename: Yaqin
authors.initials: Y
authors.lastname: Tang
authors.affiliation: School of Pharmaceutical Sciences and Innovative Drug Research Center, Chongqing University, Chongqing, 401331, China. fengxuli@cqu.edu.cn.
authors.forename: Xiao
authors.initials: X
authors.lastname: He
authors.affiliation: School of Pharmaceutical Sciences and Innovative Drug Research Center, Chongqing University, Chongqing, 401331, China. fengxuli@cqu.edu.cn.
authors.forename: Zhenxia
authors.initials: Z
authors.lastname: Zhou
authors.affiliation: School of Pharmaceutical Sciences and Innovative Drug Research Center, Chongqing University, Chongqing, 401331, China. fengxuli@cqu.edu.cn.
authors.forename: Jiakun
authors.initials: J
authors.lastname: Tang
authors.affiliation: School of Pharmaceutical Sciences and Innovative Drug Research Center, Chongqing University, Chongqing, 401331, China. fengxuli@cqu.edu.cn.
authors.forename: Rong
authors.initials: R
authors.lastname: Guo
authors.affiliation: School of Pharmaceutical Sciences and Innovative Drug Research Center, Chongqing University, Chongqing, 401331, China. fengxuli@cqu.edu.cn.
authors.forename: Xuli
authors.initials: X
authors.lastname: Feng
mesh_terms: D015374:Biosensing Techniques; D021881:DNA, Catalytic / Q000737:chemistry / Q000378:metabolism; D005456:Fluorescent Dyes / Q000737:chemistry*; D035683:MicroRNAs / Q000032:analysis* / Q000235:genetics; D011108:Polymers / Q000737:chemistry / Q000378:metabolism
chemical_list: D021881:DNA, Catalytic; D005456:Fluorescent Dyes; D035683:MicroRNAs; D011108:Polymers