title: Polyadenylated tail length variation pattern in ultra-rapid vitrified bovine oocytes.
abstract: OBJECTIVE
  Thecurrent study aims at investigating the polyadenylated (poly[A]) tail length of morphologically high and low competent oocytes at different developmental stages. Furthermore, effect of ultra-rapid vitrification on the poly(A) tail length was studied.
  
  
  METHODS
  Fresh bovine cumulus oocyte complexes from abattoir originated ovaries were graded based on morphological characters and maturedin vitro. Cryopreservation was done by ultra-rapid vitrification method. mRNA was isolated from different categories of oocyte and subjected to ligation-mediated poly(A) test followed by polymerase chain reaction for determining the poly(A) tail length of β actin, gap junction protein alpha 1 (GJA1), poly(A) polymerase alpha (PAPOLA), and heat shock 70 kDa protein (HSP70) transcripts.
  
  
  RESULTS
  GJA1, PAPOLA, and HSP70 showed significantly higher poly(A) in immature oocytes of higher competence irrespective of vitrification effects as compared to mature oocytes of higher competence.
  
  
  CONCLUSIONS
  mRNA poly(A) tail size increases in developmentally high competent immature bovine oocytes. There was limited effect of ultra-rapid vitrification of bovine oocytes on poly(A).
authors.affiliation: Department of Veterinary Physiology, Faculty of Veterinary Science, Assam Agricultural University, Khanapara, Guwahati - 781 022, Assam, India.
authors.forename: D J
authors.initials: DJ
authors.lastname: Dutta
authors.affiliation: Department of Veterinary Physiology, Faculty of Veterinary Science, Assam Agricultural University, Khanapara, Guwahati - 781 022, Assam, India.
authors.forename: Himangshu
authors.initials: H
authors.lastname: Raj
authors.affiliation: Department of Veterinary Physiology, Faculty of Veterinary Science, Assam Agricultural University, Khanapara, Guwahati - 781 022, Assam, India.
authors.forename: And Hiramoni
authors.initials: AH
authors.lastname: Dev
keywords: ligation-mediated poly(A) test; oocyte; poly-adenylated tail; vitrification