title: A protocol for rapid, label-free histochemical imaging of fibrotic liver.
abstract: Mid-infrared microscopy is a non-destructive, quantitative and label-free spectroscopic imaging technique that, as a result of recent instrument advancements, is now at the point of enabling high-throughput automated biochemical screening of whole histology samples. Currently the mid-infrared field is undergoing a paradigm shift that has not been seen since the introduction of scanning Fourier Transform interferometric spectrometers. The latest mid-infrared microscopes are powered by tunable quantum cascade laser (QCL) sources which offer a number of advantages including measurement protocol flexibility, ease-of-use and a greatly enhanced data acquisition speed at high spectral and spatial resolution. In this study we use a wide-field QCL infrared microscope to develop and validate a fast four-frequency protocol for imaging fibrosis in unstained liver tissue. We compare our results to the gold standard Masson's trichrome histochemical staining protocol.
authors.affiliation: Daylight Solutions Inc., 15378 Avenue of Science, Suite 200, San Diego, CA 92128, USA. b.bird@daylightsolutions.com.
authors.forename: B
authors.initials: B
authors.lastname: Bird
authors.forename: J
authors.initials: J
authors.lastname: Rowlette
mesh_terms: D006801:Humans; D054023:Lasers, Semiconductor; D008103:Liver Cirrhosis / Q000000981:diagnostic imaging*; D008853:Microscopy; D013194:Staining and Labeling