title: p53R2 regulates thioredoxin reductase activity through interaction with TrxR2.
abstract: Ribonucleotide reductase small subunit p53R2 is a member of the ribonucleotide reductase family that supplies dNTPs for nuclear and mitochondrial DNA replication and repair. Here, we have identified a mitochondrial thioredoxin reductase 2 (TrxR2) as a novel p53R2-binding protein. We demonstrated a direct interaction between the two, and observed that p53R2 stimulated the enzymatic activity of TrxR in vitro. Moreover, TrxR2 activity was significantly lower in p53R2 knockdown cells, and increased when p53R2 was overexpressed, effects that were independent of p53. Furthermore, p53R2 knockdown suppressed UV-induced TrxR activity. These findings suggest that p53R2 acts as a positive regulator of TrxR2 activity in mitochondria both under normal physiological conditions and during the cellular response to DNA damage.
authors.affiliation: Laboratory of Genomic Instability and Cancer Therapeutics, Cancer Mutation Research Center, Chosun University School of Medicine, 375 Seosuk-dong, Gwangju 61452, Republic of Korea; Department of Premedical Sciences, Chosun University School of Medicine, 375 Seosuk-dong, Gwangju 61452, Republic of Korea.
authors.forename: Seon-Joo
authors.initials: SJ
authors.lastname: Park
authors.affiliation: Laboratory of Genomic Instability and Cancer Therapeutics, Cancer Mutation Research Center, Chosun University School of Medicine, 375 Seosuk-dong, Gwangju 61452, Republic of Korea; Department of Premedical Sciences, Chosun University School of Medicine, 375 Seosuk-dong, Gwangju 61452, Republic of Korea.
authors.forename: Hong Beum
authors.initials: HB
authors.lastname: Kim
authors.affiliation: Laboratory of Genomic Instability and Cancer Therapeutics, Cancer Mutation Research Center, Chosun University School of Medicine, 375 Seosuk-dong, Gwangju 61452, Republic of Korea.
authors.forename: Chunmei
authors.initials: C
authors.lastname: Piao
authors.affiliation: Laboratory of Genomic Instability and Cancer Therapeutics, Cancer Mutation Research Center, Chosun University School of Medicine, 375 Seosuk-dong, Gwangju 61452, Republic of Korea.
authors.forename: Mi Yeong
authors.initials: MY
authors.lastname: Kang
authors.affiliation: Department of Internal Medicine, Hemato-Oncology, Chosun University Hospital, 375 Seosuk-dong, Gwangju 61452, Republic of Korea. Electronic address: sgpark@chosun.ac.kr.
authors.forename: Sang-Gon
authors.initials: SG
authors.lastname: Park
authors.affiliation: Department of Neurosurgery, Chosun University School of Medicine, 375 Seosuk-dong, Gwangju 61452, Republic of Korea. Electronic address: chosunns@chosun.ac.kr.
authors.forename: Seok Won
authors.initials: SW
authors.lastname: Kim
authors.affiliation: Laboratory of Genomic Instability and Cancer Therapeutics, Cancer Mutation Research Center, Chosun University School of Medicine, 375 Seosuk-dong, Gwangju 61452, Republic of Korea; Department of Cellular and Molecular Medicine, Chosun University School of Medicine, 375 Seosuk-dong, Gwangju 61452, Republic of Korea. Electronic address: jhlee75@chosun.ac.kr.
authors.forename: Jung-Hee
authors.initials: JH
authors.lastname: Lee
mesh_terms: D018797:Cell Cycle Proteins / Q000378:metabolism*; D045744:Cell Line, Tumor; D004249:DNA Damage; D004260:DNA Repair; D004272:DNA, Mitochondrial / Q000378:metabolism; D015971:Gene Expression Regulation, Enzymologic; D005822:Genetic Vectors; D006801:Humans; D008928:Mitochondria / Q000378:metabolism; D010957:Plasmids / Q000378:metabolism; D011485:Protein Binding; D034741:RNA, Small Interfering / Q000378:metabolism; D011994:Recombinant Proteins / Q000378:metabolism; D012264:Ribonucleotide Reductases / Q000378:metabolism*; D054482:Thioredoxin Reductase 2 / Q000378:metabolism*; D014466:Ultraviolet Rays; D015854:Up-Regulation
chemical_list: D018797:Cell Cycle Proteins; D004272:DNA, Mitochondrial; D034741:RNA, Small Interfering; D011994:Recombinant Proteins; C405947:RRM2B protein, human; D012264:Ribonucleotide Reductases; C516127:TXNRD2 protein, human; D054482:Thioredoxin Reductase 2
keywords: Mitochondria DNA repair; Ribonucleotide reductase; Thioredoxin reductase; TrxR2; p53R2