title: Reduced cytotoxicity in PCB-exposed Chinese Hamster Ovary (CHO) cells pretreated with vitamin E.
abstract: The aim of this study was to evaluate protective effects of vitamin E (50 -150 μM) in ovary cells upon cytotoxic effects induced by two structurally distinct PCB congeners - planar "dioxin-like" PCB 77 and non-planar di-ortho-substituted PCB 153 with an emphasis on identifying differences in the mechanism of vitamin E action depending on the structure of congeners. Application of three bioassays confirmed that PCBs decrease ovarian cell proliferation with slightly profound effects of PCB 77. PCB - induced ROS production and lipid peroxidation were significant for both congeners with also more noticeable effect for PCB 77. Vitamin E pre-incubation has improved viability of cells, reduced ROS formation and lipid peroxidation induced by PCBs' treatment. Preincubation with vitamin E was more effective when cells where treated with non-planar PCB 153. Altogether, vitamin E action was protective, congener specific and more effective when ovary cells were exposed to ortho-substituted PCB congener.
authors.affiliation: Laboratory for Toxicology, Faculty of Food Technology and Biotechnology, University of Zagreb, Pierotti St. 6, 10000 Zagreb, Croatia.
authors.forename: Teuta
authors.initials: T
authors.lastname: Murati
authors.affiliation: Laboratory for Toxicology, Faculty of Food Technology and Biotechnology, University of Zagreb, Pierotti St. 6, 10000 Zagreb, Croatia.
authors.forename: Branimir
authors.initials: B
authors.lastname: Šimić
authors.affiliation: Laboratory for Analytical Chemistry, Department of Veterinary Public Health, Croatian Veterinary Institute, Savska St. 143, 10000 Zagreb, Croatia.
authors.forename: Jelka
authors.initials: J
authors.lastname: Pleadin
authors.affiliation: Laboratory for Toxicology, Faculty of Food Technology and Biotechnology, University of Zagreb, Pierotti St. 6, 10000 Zagreb, Croatia.
authors.forename: Maja
authors.initials: M
authors.lastname: Vukmirović
authors.affiliation: Laboratory for Toxicology, Faculty of Food Technology and Biotechnology, University of Zagreb, Pierotti St. 6, 10000 Zagreb, Croatia.
authors.forename: Marina
authors.initials: M
authors.lastname: Miletić
authors.affiliation: Laboratory for Biology and Microbial Genetics, Faculty of Food Technology and Biotechnology, University of Zagreb, Pierotti St. 6, 10000 Zagreb, Croatia.
authors.forename: Ksenija
authors.initials: K
authors.lastname: Durgo
authors.affiliation: Laboratory for Toxicology, Faculty of Food Technology and Biotechnology, University of Zagreb, Pierotti St. 6, 10000 Zagreb, Croatia.
authors.forename: Jasna
authors.initials: J
authors.lastname: Kniewald
authors.affiliation: Laboratory for Toxicology, Faculty of Food Technology and Biotechnology, University of Zagreb, Pierotti St. 6, 10000 Zagreb, Croatia. Electronic address: ikmetic@pbf.hr.
authors.forename: Ivana
authors.initials: I
authors.lastname: Kmetič
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D016466:CHO Cells; D002470:Cell Survival / Q000187:drug effects*; D006224:Cricetinae; D003412:Cricetulus; D004785:Environmental Pollutants / Q000633:toxicity; D015227:Lipid Peroxidation / Q000187:drug effects*; D011078:Polychlorinated Biphenyls / Q000633:toxicity*; D017382:Reactive Oxygen Species / Q000378:metabolism*; D014810:Vitamin E / Q000494:pharmacology*
chemical_list: D000975:Antioxidants; D004785:Environmental Pollutants; D017382:Reactive Oxygen Species; D014810:Vitamin E; D011078:Polychlorinated Biphenyls
keywords: Cytotoxicity; Ovary cells; PCB 153; PCB 77; Vitamin E