title: The overexpression of KIFC1 was associated with the proliferation and prognosis of non-small cell lung cancer.
abstract: BACKGROUND
  The kinesin family member C1 (KIFC1, also known as HSET) is a kinesin superfamily protein (KIFs). Although KIFC1 acts as a crucial role in the development of several human cancers, the KIFC1 expression profile and functional remain unclear in non-small cell lung cancer (NSCLC).
  
  
  METHODS
  We collected the fresh NSCLC samples and paired normal lung tissue in patients with lung cancer operation, and detected KIFC1 expression using quantitative reverse-transcription polymerase chain reaction (qRT-PCR) and Western blotting. To expand on previous smaller-scale studies, NSCLC tissue microarrays (TMA) were analyzed by IHC. Finally, cell lines were employed to further probe the potential mechanisms.
  
  
  RESULTS
  In this study, we described that KIFC1 was significantly upregulated in NSCLC tissues compared with the corresponding normal tissues. Moreover, KIFC1 overexpression was associated with the poor overall survival (OS) of NSCLC patients, and siRNA-mediated knockdown of KIFC1 significantly suppressed tumor cell proliferationin vitro. Further verification showed that inhibition of KIFC1 gene expression caused the upregulation of the cyclin-dependent kinases inhibitor p21 and downregulation of the cell cycle driver protein cdc2, which arrested cells in the G2-M phase.
  
  
  CONCLUSIONS
  we report that increased KIFC1 expression may promote cell proliferation and identified it as a biomarker of unfavorable prognosis in NSCLC patients.
authors.affiliation: Department of Respiratory Medicine, Jinling Hospital, Southern Medical University, Nanjing 210002, China.
authors.forename: Yafang
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Department of Respiratory Medicine, Jinling Hospital, Nanjing University School of Medicine, Nanjing 210002, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Zhan
authors.affiliation: Department of Respiratory Medicine, Jinling Hospital, Southern Medical University, Nanjing 210002, China.
authors.forename: Zejun
authors.initials: Z
authors.lastname: Zhou
authors.affiliation: Department of Respiratory Medicine, Jinling Hospital, Nanjing University School of Medicine, Nanjing 210002, China.
authors.forename: Ze
authors.initials: Z
authors.lastname: Xing
authors.affiliation: Department of Respiratory Medicine, Jinling Hospital, Southern Medical University, Nanjing 210002, China.
authors.forename: Suhua
authors.initials: S
authors.lastname: Zhu
authors.affiliation: Department of Respiratory Medicine, Jinling Hospital, Southern Medical University, Nanjing 210002, China.
authors.forename: Chenhui
authors.initials: C
authors.lastname: Ma
authors.affiliation: Department of Respiratory Medicine, Jinling Hospital, Southern Medical University, Nanjing 210002, China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Li
authors.affiliation: Department of Respiratory Medicine, Jinling Hospital, Southern Medical University, Nanjing 210002, China.
authors.forename: Qingqing
authors.initials: Q
authors.lastname: Zhu
authors.affiliation: Department of Respiratory Medicine, Jinling Hospital, Southern Medical University, Nanjing 210002, China.
authors.forename: Yingying
authors.initials: Y
authors.lastname: Miao
authors.affiliation: Department of Respiratory Medicine, Jinling Hospital, Southern Medical University, Nanjing 210002, China.
authors.forename: Jianya
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Department of Respiratory Medicine, Jinling Hospital, Southern Medical University, Nanjing 210002, China;; Department of Respiratory Medicine, Jinling Hospital, Nanjing University School of Medicine, Nanjing 210002, China.
authors.forename: Tangfeng
authors.initials: T
authors.lastname: Lv
authors.affiliation: Department of Respiratory Medicine, Jinling Hospital, Southern Medical University, Nanjing 210002, China;; Department of Respiratory Medicine, Jinling Hospital, Nanjing University School of Medicine, Nanjing 210002, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Song
keywords: Kinesin family member C1 (KIFC1); cell division cycle protein 2 homolog (cdc2); cyclin-dependent kinase inhibitor 1A (p21); non-small cell lung cancer (NSCLC); prognostic