title: Characterization of theVerticillium dahliaeExoproteome Involves in Pathogenicity from Cotton-Containing Medium.
abstract: Verticilliumwilt, caused by theVerticillium dahliaephytopathogen, is a devastating disease affecting many economically important crops. Previous studies have shown that the exoproteome ofV. dahliaeplays a significant role in this pathogenic process, but the components and mechanisms that underlie this remain unclear. In this study, the exoproteome ofV. dahliaewas induced in a cotton-containing C'zapek-Dox (CCD) medium and quantified using the high-throughput isobaric tag technique for relative and absolute quantification (iTRAQ). Results showed that the abundance of 271 secreted proteins was affected by the CCD medium, of which 172 contain typical signal peptides generally produced by the Golgi/endoplasmic reticulum (ER). These enhanced abundance proteins were predominantly enriched in carbohydrate hydrolases; 126 were classified as carbohydrate-active (CAZymes) and almost all were significantly up-regulated in the CCD medium. Results showed that CAZymes proteins 30 and 22 participate in pectin and cellulose degradation pathways, corresponding with the transcription levels of several genes encoded plant cell wall degradation enzyme activated significantly during cotton infection. In addition, targeted deletion of two pectin lyase genes (VdPL3.1andVdPL3.3) impaired wilt virulence to cotton. This study demonstrates that theV. dahliaeexoproteome plays a crucial role in the development of symptoms of wilting and necrosis, predominantly via the pathogenic mechanisms of plant cell wall degradation as part of host plant infection.
authors.affiliation: Laboratory of Cotton Disease, Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences Beijing, China.
authors.forename: Jie-Yin
authors.initials: JY
authors.lastname: Chen
authors.affiliation: Laboratory of Cotton Disease, Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences Beijing, China.
authors.forename: Hong-Li
authors.initials: HL
authors.lastname: Xiao
authors.affiliation: Laboratory of Cotton Disease, Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences Beijing, China.
authors.forename: Yue-Jing
authors.initials: YJ
authors.lastname: Gui
authors.affiliation: Laboratory of Cotton Disease, Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences Beijing, China.
authors.forename: Dan-Dan
authors.initials: DD
authors.lastname: Zhang
authors.affiliation: Laboratory of Cotton Disease, Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences Beijing, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Li
authors.affiliation: Laboratory of Cotton Disease, Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences Beijing, China.
authors.forename: Yu-Ming
authors.initials: YM
authors.lastname: Bao
authors.affiliation: Laboratory of Cotton Disease, Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences Beijing, China.
authors.forename: Xiao-Feng
authors.initials: XF
authors.lastname: Dai
keywords: CAZymes; Verticillium dahliae; exoproteome; pectinases; plant cell wall degradation enzyme