title: α4β1integrin promotes accumulation of tissue-resident memory CD8+T cells in salivary glands.
abstract: The salivary glands (SGs) of virus-immune mice contain substantial numbers of tissue-resident memory CD8+T cells (TRMcells) that can provide immunity to local infections. Integrins regulate entry of activated T cells into nonlymphoid tissues but the molecules that mediate migration of virus-specific CD8+T cells to the SGs have not yet been defined. Here, we found that polyinosinic-polycytidylic acid (poly(I:C)) strongly promoted the accumulation of P14 TCR-transgenic CD8+TRMcells in SGs in an α4β1integrin-dependent manner. After infection with lymphocytic choriomeningitis virus, accumulation of P14 TRMcells in SGs and intestine but not in kidney was also α4integrin dependent. Blockade of α4β7by monoclonal antibodies (mAbs) inhibited lymphocytic choriomeningitis virus-induced accumulation of P14 TRMcells in the intestine but not in SGs. In conclusion, our data reveal that α4β1integrin mediates CD8+TRMaccumulation in SGs and that poly(I:C) can be used to direct activated CD8+T cells to this organ.
authors.affiliation: Institute for Immunology, University Medical Center Freiburg, Freiburg, Germany.|Faculty of Biology, Albert Ludwigs University of Freiburg, Freiburg, Germany.
authors.forename: Sandra
authors.initials: S
authors.lastname: Woyciechowski
authors.affiliation: Institute for Immunology, University Medical Center Freiburg, Freiburg, Germany.
authors.forename: Maike
authors.initials: M
authors.lastname: Hofmann
authors.affiliation: Institute for Immunology, University Medical Center Freiburg, Freiburg, Germany.
authors.forename: Hanspeter
authors.initials: H
authors.lastname: Pircher
mesh_terms: D019264:Adoptive Transfer; D000818:Animals; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D002465:Cell Movement / Q000235:genetics; D002478:Cells, Cultured; D007156:Immunologic Memory; D039041:Integrin alpha4beta1 / Q000235:genetics / Q000378:metabolism*; D008216:Lymphocytic Choriomeningitis / Q000276:immunology*; D008217:Lymphocytic choriomeningitis virus / Q000276:immunology*; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D011070:Poly I-C / Q000276:immunology; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000235:genetics; D054367:Receptors, CXCR3 / Q000235:genetics / Q000378:metabolism; D016201:Receptors, Lymphocyte Homing / Q000235:genetics / Q000378:metabolism*; D012469:Salivary Glands / Q000276:immunology*; D016176:T-Lymphocyte Subsets / Q000276:immunology*
chemical_list: C515188:Cxcr3 protein, mouse; D039041:Integrin alpha4beta1; D016693:Receptors, Antigen, T-Cell, alpha-beta; D054367:Receptors, CXCR3; D016201:Receptors, Lymphocyte Homing; D011070:Poly I-C
keywords: Salivary glands; Tissue-resident memory T cells (TRM cells); VCAM-1; Viral infections; α4β1 integrin