title: Usefulness of toxicological validation of VOCs catalytic degradation by air-liquid interface exposure system.
abstract: Toluene is one of the most used Volatile Organic Compounds (VOCs) in the industry despite its major health impacts. Catalytic oxidation represents an efficient remediation technique in order to reduce its emission directly at the source, but it can release by-products. To complete the classical performance assessment using dedicated analytical chemistry methods, we propose to perform an untargeted toxicological validation on two efficient catalysts. Using biological system allows integrating synergy and antagonism in toxic effects of emitted VOCs and by-products, often described in case of multi-exposure condition. Catalysts Pd/α-Al2O3and Pd/γ-Al2O3developed for the oxidation of toluene were both coupled to a Vitrocell®Air-Liquid Interface (ALI) system, for exposure of human A549 lung cells during 1h to toluene or to catalysts exhaust before quantification of xenobiotics metabolizing enzymes. This study validated initially the Vitrocell®as an innovative, direct and dynamic model of ALI exposure in the assessment of the performances of new catalysts, showing the presence of chemically undetected by-products. The comparison of the two catalysts showed then that fewer organic compounds metabolizing genes were induced by Pd/γ-Al2O3in comparison to Pd/α-Al2O3, suggesting that Pd/γ-Al2O3is more efficient for toluene total oxidation from a toxicological point of view.
authors.affiliation: Unité de Chimie Environnementale et Interactions sur le Vivant EA4492, Université du Littoral Côte d'Opale, 189A Avenue Maurice Schumann, 59140 Dunkerque, France.
authors.forename: Margueritta
authors.initials: M
authors.lastname: Al Zallouha
authors.affiliation: Unité de Chimie Environnementale et Interactions sur le Vivant EA4492, Université du Littoral Côte d'Opale, 189A Avenue Maurice Schumann, 59140 Dunkerque, France.
authors.forename: Yann
authors.initials: Y
authors.lastname: Landkocz
authors.affiliation: Unité de Chimie Environnementale et Interactions sur le Vivant EA4492, Université du Littoral Côte d'Opale, 189A Avenue Maurice Schumann, 59140 Dunkerque, France.
authors.forename: Julien
authors.initials: J
authors.lastname: Brunet
authors.affiliation: Unité de Chimie Environnementale et Interactions sur le Vivant EA4492, Université du Littoral Côte d'Opale, 189A Avenue Maurice Schumann, 59140 Dunkerque, France.
authors.forename: Renaud
authors.initials: R
authors.lastname: Cousin
authors.affiliation: Unité de Chimie Environnementale et Interactions sur le Vivant EA4492, Université du Littoral Côte d'Opale, 189A Avenue Maurice Schumann, 59140 Dunkerque, France.
authors.forename: Eric
authors.initials: E
authors.lastname: Genty
authors.affiliation: Unité de Chimie Environnementale et Interactions sur le Vivant EA4492, Université du Littoral Côte d'Opale, 189A Avenue Maurice Schumann, 59140 Dunkerque, France.
authors.forename: Dominique
authors.initials: D
authors.lastname: Courcot
authors.affiliation: Unité de Chimie Environnementale et Interactions sur le Vivant EA4492, Université du Littoral Côte d'Opale, 189A Avenue Maurice Schumann, 59140 Dunkerque, France.
authors.forename: Stéphane
authors.initials: S
authors.lastname: Siffert
authors.affiliation: Unité de Chimie Environnementale et Interactions sur le Vivant EA4492, Université du Littoral Côte d'Opale, 189A Avenue Maurice Schumann, 59140 Dunkerque, France.
authors.forename: Pirouz
authors.initials: P
authors.lastname: Shirali
authors.affiliation: Unité de Chimie Environnementale et Interactions sur le Vivant EA4492, Université du Littoral Côte d'Opale, 189A Avenue Maurice Schumann, 59140 Dunkerque, France. Electronic address: Sylvain.Billet@univ-littoral.fr.
authors.forename: Sylvain
authors.initials: S
authors.lastname: Billet
mesh_terms: D000537:Aluminum Oxide / Q000737:chemistry*; D002384:Catalysis; D004785:Environmental Pollutants / Q000737:chemistry*; D052918:Environmental Restoration and Remediation / Q000295:instrumentation / Q000379:methods*; D010084:Oxidation-Reduction; D010165:Palladium / Q000737:chemistry*; D014050:Toluene / Q000737:chemistry*; D055549:Volatile Organic Compounds / Q000737:chemistry
chemical_list: D004785:Environmental Pollutants; D055549:Volatile Organic Compounds; D014050:Toluene; D010165:Palladium; D000537:Aluminum Oxide
keywords: Air-liquid interface exposure; By-products identification; Catalytic oxidation; Toluene; Toxicological validation