title: Outcome of transplanted donor corneas with more than 6 h of death-to-preservation time.
abstract: OBJECTIVE
  In tropical countries, physicians are skeptic in using corneas with death-to-preservation time (DTPT) >6 h, concerns being endothelial cell viability and microbial contamination on prolonged DTPT. The objective of the study was to investigate these concerns by analyzing the outcomes of corneal transplants performed using donor corneas with DTPT >6 h.
  
  
  METHODS
  The study was a retrospective case series of 65 transplants performed in 2013 with donor corneas that had DTPT >6 h (range, 6.1-9.8 h). The information on donor cornea tissues and the recipient details were collected from the eye bank and the medical records department of our tertiary eye care center. The main outcome measures were slit lamp assessment of the donor corneas, primary graft failure, graft survival, and postoperative adverse reactions, especially infections, if any.
  
  
  RESULTS
  Median DTPT was 7 h. Forty-four (67.7%) corneas were evaluated as optical grade and 21 (32.3%) were deemed as therapeutic grade; 36 (55.4%) were used for optical indications. There was no relationship between DTPT and the tissue grading of corneas or endothelial cell density. Of the 23 keratoplasties for purely optical indications with a minimum follow-up of 3 months, 15 (65.2%) remained clear whereas 7 (30.4%) failed (mean follow-up 15.1 ± 6.7 months). The causes of failure were primary graft failure (n = 1) and secondary graft failure (n = 6).
  
  
  CONCLUSIONS
  The donor corneas with DTPT 6 h to 10 h can be utilized for optical indications provided that they meet the criteria of tissue acceptance for optical use.
authors.affiliation: Ophthalmic Biophysics, L. V. Prasad Eye Institute, Hyderabad, Telangana, India.
authors.forename: Ashik
authors.initials: A
authors.lastname: Mohamed
authors.affiliation: Cornea and Anterior Segment Services, L. V. Prasad Eye Institute, Hyderabad, Telangana, India.
authors.forename: Sunita
authors.initials: S
authors.lastname: Chaurasia
authors.affiliation: Cornea and Anterior Segment Services, L. V. Prasad Eye Institute, Hyderabad, Telangana, India.
authors.forename: Prashant
authors.initials: P
authors.lastname: Garg
mesh_terms: D000328:Adult; D000368:Aged; D003315:Cornea; D003316:Corneal Diseases / Q000503:physiopathology / Q000601:surgery; D005125:Eye Banks / Q000706:statistics & numerical data; D005260:Female; D006085:Graft Survival / Q000502:physiology*; D006801:Humans; D015948:Keratoplasty, Penetrating; D008297:Male; D008875:Middle Aged; D012189:Retrospective Studies; D013997:Time Factors; D014019:Tissue Donors; D014021:Tissue Preservation; D020858:Tissue and Organ Harvesting; D016896:Treatment Outcome