title: Cell cycle arrest and gene expression profiling of testis in mice exposed to fluoride.
abstract: Exposure to fluoride results in low reproductive capacity; however, the mechanism underlying the impact of fluoride on male productive system still remains obscure. To assess the potential toxicity in testis of mice administrated with fluoride, global genome microarray and real-time PCR were performed to detect and identify the altered transcriptions. The results revealed that 763 differentially expressed genes were identified, including 330 up-regulated and 433 down-regulated genes, which were involved in spermatogenesis, apoptosis, DNA damage, DNA replication, and cell differentiation. Twelve differential expressed genes were selected to confirm the microarray results using real-time PCR, and the result kept the same tendency with that of microarray. Furthermore, compared with the control group, more apoptotic spermatogenic cells were observed in the fluoride group, and the spermatogonium were markedly increased in S phase and decreased in G2/M phase by fluoride. Our findings suggested global genome microarray provides an insight into the reproductive toxicity induced by fluoride, and several important biological clues for further investigations. © 2016 Wiley Periodicals, Inc. Environ Toxicol 32: 1558-1565, 2017.
authors.affiliation: College of Animal Science and Veterinary Medicine, Shanxi Key Laboratory of Ecological Animal Science and Environmental Veterinary Medicine, Shanxi Agricultural University, Taigu, Shanxi, 030801, People's Republic of China.|Taiyuan Zoo, Taiyuan, Shanxi, 030009, People's Republic of China.
authors.forename: Kai
authors.initials: K
authors.lastname: Su
authors.affiliation: College of Animal Science and Veterinary Medicine, Shanxi Key Laboratory of Ecological Animal Science and Environmental Veterinary Medicine, Shanxi Agricultural University, Taigu, Shanxi, 030801, People's Republic of China.
authors.forename: Zilong
authors.initials: Z
authors.lastname: Sun
authors.affiliation: College of Animal Science and Veterinary Medicine, Shanxi Key Laboratory of Ecological Animal Science and Environmental Veterinary Medicine, Shanxi Agricultural University, Taigu, Shanxi, 030801, People's Republic of China.
authors.forename: Ruiyan
authors.initials: R
authors.lastname: Niu
authors.affiliation: Taiyuan Zoo, Taiyuan, Shanxi, 030009, People's Republic of China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Lei
authors.affiliation: Medical Systems Biology Research Center, Department of Biomedical Engineering, Tsinghua University School of Medicine, Beijing, 100084, People's Republic of China.
authors.forename: Jing
authors.initials: J
authors.lastname: Cheng
authors.affiliation: College of Animal Science and Veterinary Medicine, Shanxi Key Laboratory of Ecological Animal Science and Environmental Veterinary Medicine, Shanxi Agricultural University, Taigu, Shanxi, 030801, People's Republic of China.
authors.forename: Jundong
authors.initials: J
authors.lastname: Wang
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects / Q000235:genetics; D059447:Cell Cycle Checkpoints / Q000187:drug effects* / Q000235:genetics; D002454:Cell Differentiation / Q000187:drug effects / Q000235:genetics; D004249:DNA Damage / Q000187:drug effects / Q000235:genetics; D004781:Environmental Exposure / Q000032:analysis; D005459:Fluorides / Q000633:toxicity*; D020869:Gene Expression Profiling; D005854:Germ Cells / Q000187:drug effects / Q000378:metabolism; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D046228:Microarray Analysis; D010710:Phosphates / Q000633:toxicity; D012098:Reproduction / Q000187:drug effects; D013091:Spermatogenesis / Q000187:drug effects / Q000235:genetics; D013737:Testis / Q000187:drug effects* / Q000378:metabolism; D059467:Transcriptome / Q000187:drug effects*
chemical_list: D010710:Phosphates; C012980:fluorophosphate; D005459:Fluorides
keywords: cell cycle; fluoride; global genome microarray; mice; testis