title: In VitroAnti-Inflammatory Effects of Three Fatty Acids from Royal Jelly.
abstract: Trans-10-hydroxy-2-decenoic acid (10-H2DA), 10-hydroxydecanoic acid (10-HDAA), and sebacic acid (SEA) are the three major fatty acids in royal jelly (RJ). Previous studies have revealed several pharmacological activities of 10-H2DA and 10-HDAA, although the anti-inflammatory effects and underlying mechanisms by which SEA acts are poorly understood. In the present study, we evaluated and compared thein vitroanti-inflammatory effects of these RJ fatty acids in lipopolysaccharide-stimulated RAW 264.7 macrophages. The results showed that 10-H2DA, 10-HDAA, and SEA had potent, dose-dependent inhibitory effects on the release of the major inflammatory-mediators, nitric oxide, and interleukin-10, and only SEA decreased TNF-αproduction. Several key inflammatory genes have also been modulated by these RJ fatty acids, with 10-H2DA showing distinct modulating effects as compared to the other two FAs. Furthermore, we found that these three FAs regulated several proteins involved in MAPK and NF-κB signaling pathways. Taken together, these findings provide additional references for using RJ against inflammatory diseases.
authors.affiliation: College of Animal Sciences, Zhejiang University, Hangzhou 310058, China.
authors.forename: Yi-Fan
authors.identifier: 0000-0003-2628-5764
authors.initials: YF
authors.lastname: Chen
authors.affiliation: Institute of Apicultural Research, Chinese Academy of Agricultural Sciences, Beijing 100093, China.
authors.forename: Kai
authors.identifier: 0000-0003-0553-5293
authors.initials: K
authors.lastname: Wang
authors.affiliation: College of Animal Sciences, Zhejiang University, Hangzhou 310058, China.
authors.forename: Yan-Zheng
authors.initials: YZ
authors.lastname: Zhang
authors.affiliation: College of Animal Sciences, Zhejiang University, Hangzhou 310058, China.
authors.forename: Yu-Fei
authors.initials: YF
authors.lastname: Zheng
authors.affiliation: College of Animal Sciences, Zhejiang University, Hangzhou 310058, China.
authors.forename: Fu-Liang
authors.identifier: 0000-0002-2457-7475
authors.initials: FL
authors.lastname: Hu
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000737:chemistry*; D002470:Cell Survival; D016207:Cytokines / Q000378:metabolism; D003652:Decanoic Acids / Q000737:chemistry*; D003998:Dicarboxylic Acids / Q000737:chemistry*; D005227:Fatty Acids / Q000737:chemistry*; D005229:Fatty Acids, Monounsaturated / Q000737:chemistry*; D007249:Inflammation; D008070:Lipopolysaccharides; D020935:MAP Kinase Signaling System; D008264:Macrophages / Q000166:cytology / Q000378:metabolism; D051379:Mice; D016328:NF-kappa B / Q000378:metabolism; D009569:Nitric Oxide / Q000378:metabolism; D000067996:RAW 264.7 Cells; D015398:Signal Transduction; D000099219:Royal Jelly
chemical_list: D000893:Anti-Inflammatory Agents; D016207:Cytokines; D003652:Decanoic Acids; D003998:Dicarboxylic Acids; D005227:Fatty Acids; D005229:Fatty Acids, Monounsaturated; D008070:Lipopolysaccharides; D016328:NF-kappa B; D009569:Nitric Oxide; D000099219:Royal Jelly; C000621793:10-hydroxydecanoic acid; C055543:10-hydroxy-2-decenoic acid; C011107:sebacic acid