title: Structure elucidation and analysis of biosynthesis genes of the O-antigen of Escherichia coli O131 containing N-acetylneuraminic acid.
abstract: The O-polysaccharide (O-antigen) of Escherichia coli O131 was studied by sugar analysis along with 1D and 2D1H and13C NMR spectroscopy. The following structure of the linear tetrasaccharide repeating unit of the polysaccharide was established: →8)-α-Neup5Ac-(2 → 6)-β-D-Galp-(1 → 6)-β-D-Galp-(1 → 3)-β-D-GalpNAc-(1→ The gene functions were tentatively assigned by comparison with sequences in the available databases and found to be in agreement with the E. coli O131-antigen structure.
authors.affiliation: N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 119991, Moscow, Russian Federation. Electronic address: perepel@ioc.ac.ru.
authors.forename: Andrei V
authors.initials: AV
authors.lastname: Perepelov
authors.affiliation: Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, TEDA Institute of Biological Sciences and Biotechnology, Nankai University, TEDA, Tianjin, China.
authors.forename: Xi
authors.initials: X
authors.lastname: Guo
authors.affiliation: N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 119991, Moscow, Russian Federation.
authors.forename: Sof'ya N
authors.initials: SN
authors.lastname: Senchenkova
authors.affiliation: N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 119991, Moscow, Russian Federation.
authors.forename: Alexander S
authors.initials: AS
authors.lastname: Shashkov
authors.affiliation: Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, TEDA Institute of Biological Sciences and Biotechnology, Nankai University, TEDA, Tianjin, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Liu
authors.affiliation: N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 119991, Moscow, Russian Federation.
authors.forename: Yuriy A
authors.initials: YA
authors.lastname: Knirel
mesh_terms: D002240:Carbohydrate Sequence; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism; D005798:Genes, Bacterial / Q000235:genetics*; D009682:Magnetic Resonance Spectroscopy; D019158:N-Acetylneuraminic Acid / Q000378:metabolism*; D019081:O Antigens / Q000737:chemistry* / Q000235:genetics*; D009844:Oligosaccharides / Q000737:chemistry*
chemical_list: D019081:O Antigens; D009844:Oligosaccharides; D019158:N-Acetylneuraminic Acid
keywords: Bacterial polysaccharide structure; Escherichia coli; Lipopolysaccharide; N-acetylneuraminic acid; O-antigen gene cluster; O-polysaccharide structure