title: Gene expression profiling of breast cancer in Lebanese women.
abstract: Breast cancer is commonest cancer in women worldwide. Elucidation of underlying biology and molecular pathways is necessary for improving therapeutic options and clinical outcomes. Molecular alterations in breast cancer are complex and involve cross-talk between multiple signaling pathways. The aim of this study is to extract a unique mRNA fingerprint of breast cancer in Lebanese women using microarray technologies. Gene-expression profiles of 94 fresh breast tissue samples (84 cancerous/10 non-tumor adjacent samples) were analyzed using GeneChip Human Genome U133 Plus 2.0 arrays. Quantitative real-time PCR was employed to validate candidate genes. Differentially expressed genes between breast cancer and non-tumor tissues were screened. Significant differences in gene expression were established for COL11A1/COL10A1/MMP1/COL6A6/DLK1/S100P/CXCL11/SOX11/LEP/ADIPOQ/OXTR/FOSL1/ACSBG1 and C21orf37. Pathways/diseases representing these genes were retrieved and linked using PANTHER®/Pathway Studio®. Many of the deregulated genes are associated with extracellular matrix, inflammation, angiogenesis, metastasis, differentiation, cell proliferation and tumorigenesis. Characteristics of breast cancers in Lebanese were compared to those of women from Western populations to explain why breast cancer is more aggressive and presents a decade earlier in Lebanese victims. Delineating molecular mechanisms of breast cancer in Lebanese women led to key genes which could serve as potential biomarkers and/or novel drug targets for breast cancer.
authors.affiliation: Department of Biochemistry and Molecular Genetics, American University of Beirut Medical Center, Beirut, Lebanon.
authors.forename: Joelle
authors.initials: J
authors.lastname: Makoukji
authors.affiliation: Department of Biochemistry and Molecular Genetics, American University of Beirut Medical Center, Beirut, Lebanon.
authors.forename: Nadine J
authors.initials: NJ
authors.lastname: Makhoul
authors.affiliation: Department of Internal Medicine, University of Miami Miller SOM Regional Campus, 5301 S. Congress Avenue, Atlantis City, Fl 33462, US.
authors.forename: Maya
authors.initials: M
authors.lastname: Khalil
authors.affiliation: Department of Biochemistry and Molecular Genetics, American University of Beirut Medical Center, Beirut, Lebanon.
authors.forename: Sally
authors.initials: S
authors.lastname: El-Sitt
authors.affiliation: Department of Radiology, University of Iowa Hospitals and Clinics, 200 Hawkins Drive, Iowa City, IA 52242, US.
authors.forename: Ehab Saad
authors.initials: ES
authors.lastname: Aldin
authors.affiliation: Department of Pathology and Laboratory Medicine, American University of Beirut Medical Center, Beirut, Lebanon.
authors.forename: Mark
authors.initials: M
authors.lastname: Jabbour
authors.affiliation: Department of Pathology and Laboratory Medicine, American University of Beirut Medical Center, Beirut, Lebanon.
authors.forename: Fouad
authors.initials: F
authors.lastname: Boulos
authors.affiliation: Centre for Molecular Oncology, Barts Cancer Institute, Queen Mary University of London, Charterhouse Square, London EC1M 6BQ, UK.
authors.forename: Emanuela
authors.initials: E
authors.lastname: Gadaleta
authors.affiliation: Department of Pathology and Laboratory Medicine, American University of Beirut Medical Center, Beirut, Lebanon.
authors.forename: Ajanthah
authors.initials: A
authors.lastname: Sangaralingam
authors.affiliation: Centre for Molecular Oncology, Barts Cancer Institute, Queen Mary University of London, Charterhouse Square, London EC1M 6BQ, UK.
authors.forename: Claude
authors.initials: C
authors.lastname: Chelala
authors.affiliation: Department of Biochemistry and Molecular Genetics, American University of Beirut Medical Center, Beirut, Lebanon.|Neurogenetics Program and Division of Pediatric Neurology, Department of Pediatrics and Adolescent Medicine, American University of Beirut Medical Center, Beirut, Lebanon.
authors.forename: Rose-Mary
authors.initials: RM
authors.lastname: Boustany
authors.affiliation: Department of Internal Medicine, American University of Beirut Medical Center, Beirut, Lebanon.
authors.forename: Arafat
authors.initials: A
authors.lastname: Tfayli
mesh_terms: D001943:Breast Neoplasms / Q000208:ethnology / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D005260:Female; D020869:Gene Expression Profiling; D006801:Humans; D007861:Lebanon / Q000208:ethnology; D008875:Middle Aged; D020411:Oligonucleotide Array Sequence Analysis; D060066:Protein Interaction Maps; D060888:Real-Time Polymerase Chain Reaction; D015398:Signal Transduction