title: Effects of normobaricversushyperbaric oxygen on cell injury induced by oxygen and glucose deprivation in acute brain slices.
abstract: Normobaric oxygen (NBO) and hyperbaric oxygen (HBO) are emerging as a possible co-treatment of acute ischemic stroke. Both have been shown to reduce infarct volume, to improve neurologic outcome, to promote endogenous tissue plasminogen activator-induced thrombolysis and cerebral blood flow, and to improve tissue oxygenation through oxygen diffusion in the ischemic areas, thereby questioning the interest of HBO compared to NBO. In the present study, in order to investigate and compare the oxygen diffusion effects of NBO and HBO on acute ischemic stroke independently of their effects at the vascular level, we used acute brain slices exposed to oxygen and glucose deprivation, anex vivomodel of brain ischemia that allows investigating the acute effects of NBO (partial pressure of oxygen (pO2) = 1 atmospheres absolute (ATA) = 0.1 MPa) and HBO (pO2= 2.5 ATA = 0.25 MPa) through tissue oxygenation on ischemia-induced cell injury as measured by the release of lactate dehydrogenase. We found that HBO, but not NBO, reduced oxygen and glucose deprivation-induced cell injury, indicating that passive tissue oxygenation (i.e. without vascular support) of the brain parenchyma requires oxygen partial pressure higher than 1 ATA.
authors.affiliation: Normandie Université, UNICAEN, CNRS, UMR 6301 ISTCT, Equipe Cervoxy, Caen, France.
authors.forename: Laurent
authors.initials: L
authors.lastname: Chazalviel
authors.affiliation: Hôpital d'Instruction des Armées (HIA) Sainte-Anne, Service de Médecine Hyperbare et Expertise Plongée (SMHEP), Toulon, France.
authors.forename: Jean-Eric
authors.initials: JE
authors.lastname: Blatteau
authors.affiliation: Institut de Recherche Biomédicale des Armées (IRBA), Equipe Résidente de Recherche Subaquatique Opérationnelle (ERRSO), Toulon, France.
authors.forename: Nicolas
authors.initials: N
authors.lastname: Vallée
authors.affiliation: Institut de Recherche Biomédicale des Armées (IRBA), Equipe Résidente de Recherche Subaquatique Opérationnelle (ERRSO), Toulon, France.
authors.forename: Jean-Jacques
authors.initials: JJ
authors.lastname: Risso
authors.affiliation: Normandie Université, UNICAEN, INSERM, UMR 1075, Caen, France.
authors.forename: Stéphane
authors.initials: S
authors.lastname: Besnard
authors.affiliation: Institut de Recherche Biomédicale des Armées (IRBA), Equipe Résidente de Recherche Subaquatique Opérationnelle (ERRSO), Toulon, France; Normandie Université, UNICAEN, Faculté de Médecine, France; Université Laval, Département d'Anesthésiologie, Québec, Canada.
authors.forename: Jacques H
authors.identifier: 0000-0002-6435-9819
authors.initials: JH
authors.lastname: Abraini
keywords: brain ischemia; brain slices; cell injury; hyperbaric oxygen; lactate dehydogenase; normobaric oxygen; oxygen and glucose deprivation; oxygen diffusion