title: Aquamicrobium soli sp. nov., a bacterium isolated from a chlorobenzoate-contaminated soil.
abstract: An aerobic, Gram-stain negative, short rod-shaped, asporogenous, non-motile bacterium designated strain NK8Twas isolated from a chlorobenzoate contaminated soil in China. Strain NK8Twas observed to grow optimally at pH 7.0, 30 °C and in the absence of NaCl in LB medium. The G + C content of the total DNA of strain NK8Twas found to be 65.5 mol%. The 16S rRNA gene sequence of strain NK8Tshowed high similarity to that of Aquamicrobium aerolatum Sa14T(97.3%), followed by Aquamicrobium lusatiense S1T(96.7%) and Mesorhizobium sangali SCAU7T(96.6%). The DNA-DNA relatedness between strain NK8Tand A. aerolatum Sa14Twas 35.5 ± 0.9%. The major fatty acids of strain NK8Twere determined to be C19:0cyclo ω8c (45.6%), C18:1ω7c (33.4%) and C16:0(8.4%). The respiratory quinone was found to be ubiquinone Q-10. The major polyamine was found to be spermidine. The polar lipid profile include the major compounds phosphatidylcholine and diphosphatidylglycerol, and moderate amounts of phosphatidylethanolamine, phosphatidylmonomethylethanolamine, aminolipid and phospholipid. Based on the differential biochemical and physiological characteristics, the geno-, chemo- and phenotypic characteristics, strain NK8Tis proposed to represent a novel species of the genus Aquamicrobium, Aquamicrobium soli sp. nov. The type strain is NK8T(=KCTC 52165T=CCTCC AB2016045T).
authors.affiliation: Department of Microbiology, Key Lab of Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, College of Life Sciences, Nanjing Agricultural University, Nanjing, 210095, People's Republic of China.
authors.forename: Chang-Feng
authors.initials: CF
authors.lastname: Xu
authors.affiliation: Department of Microbiology, Key Lab of Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, College of Life Sciences, Nanjing Agricultural University, Nanjing, 210095, People's Republic of China.
authors.forename: Long
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Department of Microbiology, Key Lab of Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, College of Life Sciences, Nanjing Agricultural University, Nanjing, 210095, People's Republic of China.
authors.forename: Jun-Wei
authors.initials: JW
authors.lastname: Huang
authors.affiliation: Department of Microbiology, Key Lab of Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, College of Life Sciences, Nanjing Agricultural University, Nanjing, 210095, People's Republic of China.
authors.forename: Kai
authors.initials: K
authors.lastname: Chen
authors.affiliation: Department of Microbiology, Key Lab of Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, College of Life Sciences, Nanjing Agricultural University, Nanjing, 210095, People's Republic of China.
authors.forename: Shun-Peng
authors.initials: SP
authors.lastname: Li
authors.affiliation: Department of Microbiology, Key Lab of Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, College of Life Sciences, Nanjing Agricultural University, Nanjing, 210095, People's Republic of China. jiang_jjd@njau.edu.cn.
authors.forename: Jian-Dong
authors.initials: JD
authors.lastname: Jiang
mesh_terms: D002681:China; D002712:Chlorides / Q000378:metabolism; D002723:Chlorobenzoates / Q000737:chemistry*; D004269:DNA, Bacterial / Q000235:genetics; D004787:Environmental Pollution; D005227:Fatty Acids / Q000032:analysis; D010743:Phospholipids / Q000032:analysis; D059127:Phyllobacteriaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D010802:Phylogeny; D011809:Quinones / Q000032:analysis; D012987:Soil / Q000737:chemistry; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000737:chemistry*; D013045:Species Specificity; D014451:Ubiquinone / Q000031:analogs & derivatives / Q000032:analysis
chemical_list: D002712:Chlorides; D002723:Chlorobenzoates; D004269:DNA, Bacterial; D005227:Fatty Acids; D010743:Phospholipids; D011809:Quinones; D012987:Soil; D012989:Soil Pollutants; D014451:Ubiquinone; C024989:coenzyme Q10; C001599:chlorite
keywords: 16S rRNA gene; Aquamicrobium soli sp. nov.; Gram-stain negative; Taxonomy