title: Complete genome sequence of a non-pathogenic strain of Fowl Adenovirus serotype 11: Minimal genomic differences between pathogenic and non-pathogenic viruses.
abstract: In this study, we conducted the clinicopathological characterization of a non-pathogenic FAdV-D serotype 11 strain MX95, isolated from healthy chickens, and its entire genome was sequenced. Experiments in SPF chickens revealed that the strain is a non-pathogenic virus that did not cause death at challenge doses of 1×106TCID50. Additionally, the infection in SPF chickens caused no apparent damage in most of the organs analyzed by necropsy and histopathology, but it did cause inclusion body hepatitis; nevertheless it did not generate severe infectious clinical symptoms. The virus was detected in several chicken organs, including the lymphoid organs, by real-time polymerase chain reaction (PCR) until 42 days. The genome of FAdV-11 MX95 has a size of 44,326bp, and it encodes 36 open reading frames (ORFs). Comparative analysis of the genome indicated only 0.8% dissimilarity with a highly virulent serotype 11 that was previously reported.
authors.affiliation: Instituto Politécnico Nacional, Centro de Investigación en Biotecnología Aplicada-Unidad Tlaxcala, Carr. Est. Santa Inés Tecuexcomac-Tepetitla Km. 1.5, Tepetitla, Tlaxcala CP 90700, Mexico. Electronic address: aabsalon@ipn.mx.
authors.forename: Angel E
authors.initials: AE
authors.lastname: Absalón
authors.affiliation: Investigación Aplicada S.A. de C.V., 7 Norte No. 416 Col. Centro, Tehuacán, Puebla CP 75740, Mexico.
authors.forename: Andrés
authors.initials: A
authors.lastname: Morales-Garzón
authors.affiliation: Instituto Politécnico Nacional, Centro de Investigación en Biotecnología Aplicada-Unidad Tlaxcala, Carr. Est. Santa Inés Tecuexcomac-Tepetitla Km. 1.5, Tepetitla, Tlaxcala CP 90700, Mexico.
authors.forename: Pedro F
authors.initials: PF
authors.lastname: Vera-Hernández
authors.affiliation: Instituto Politécnico Nacional, Centro de Investigación en Biotecnología Aplicada-Unidad Tlaxcala, Carr. Est. Santa Inés Tecuexcomac-Tepetitla Km. 1.5, Tepetitla, Tlaxcala CP 90700, Mexico.
authors.forename: Diana V
authors.initials: DV
authors.lastname: Cortés-Espinosa
authors.affiliation: Instituto Politécnico Nacional, Centro de Investigación en Biotecnología Aplicada-Unidad Tlaxcala, Carr. Est. Santa Inés Tecuexcomac-Tepetitla Km. 1.5, Tepetitla, Tlaxcala CP 90700, Mexico.
authors.forename: Sara M
authors.initials: SM
authors.lastname: Uribe-Ochoa
authors.affiliation: Instituto Politécnico Nacional, Centro de Investigación en Biotecnología Aplicada-Unidad Tlaxcala, Carr. Est. Santa Inés Tecuexcomac-Tepetitla Km. 1.5, Tepetitla, Tlaxcala CP 90700, Mexico.
authors.forename: Laura J
authors.initials: LJ
authors.lastname: García
authors.affiliation: Investigación Aplicada S.A. de C.V., 7 Norte No. 416 Col. Centro, Tehuacán, Puebla CP 75740, Mexico.
authors.forename: Eduardo
authors.initials: E
authors.lastname: Lucio-Decanini
mesh_terms: D000257:Adenoviridae Infections / Q000473:pathology / Q000662:veterinary* / Q000821:virology; D000595:Amino Acid Sequence; D000818:Animals; D000259:Aviadenovirus / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification / Q000472:pathogenicity*; D001483:Base Sequence; D002645:Chickens; D016679:Genome, Viral; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D011201:Poultry Diseases / Q000473:pathology / Q000821:virology*; D016415:Sequence Alignment; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics
chemical_list: D014764:Viral Proteins
keywords: FAdV-11; Fowl Adenovirus; Hepatitis-hydropericardium syndrome; Inclusion bodies hepatitis; Low pathogenic Adenovirus