title: The Rip1Tag2 Transgenic Mouse Model.
abstract: The Rip1Tag2 transgenic mouse model of β-cell carcinogenesis has been instrumental in studying various aspects of tumor angiogenesis and in investigating the response to anti-angiogenic therapeutics. Thereby, the in-depth assessment of blood and lymphatic vessel phenotypes and functionality represents key experimental analyses. In this chapter, we describe basic protocols to assess tumor blood vessel morphology (pericyte coverage), functionality (perfusion, leakiness, and hypoxia), lymphatic tumor coverage, and tumor cell proliferation and apoptosis based on immunofluorescence microscopy analysis.
authors.affiliation: Department of Biomedicine, University of Basel, Mattenstrasse 28, 4058, Basel, Switzerland.
authors.forename: Ruben
authors.initials: R
authors.lastname: Bill
authors.affiliation: Department of Biomedicine, University of Basel, Mattenstrasse 28, 4058, Basel, Switzerland. gerhard.christofori@unibas.ch.
authors.forename: Gerhard
authors.initials: G
authors.lastname: Christofori
mesh_terms: D000818:Animals; D000957:Antigens, Viral, Tumor / Q000235:genetics; D017209:Apoptosis; D018278:Carcinoma, Neuroendocrine / Q000098:blood supply / Q000235:genetics* / Q000473:pathology; D049109:Cell Proliferation; D007328:Insulin / Q000235:genetics; D007340:Insulinoma / Q000098:blood supply / Q000235:genetics* / Q000473:pathology; D051379:Mice; D008822:Mice, Transgenic; D009374:Neoplasms, Experimental / Q000098:blood supply / Q000235:genetics; D009389:Neovascularization, Pathologic / Q000235:genetics / Q000473:pathology*; D010190:Pancreatic Neoplasms / Q000098:blood supply / Q000235:genetics* / Q000473:pathology; D011401:Promoter Regions, Genetic
chemical_list: D000957:Antigens, Viral, Tumor; D007328:Insulin
keywords: Anti-angiogenic therapy; Endothelial cells; Immunofluorescence; Insulinoma; PNET; Rip1Tag2; Tumor angiogenesis