title: Selenium Nanoparticles Attenuate Oxidative Stress and Testicular Damage in Streptozotocin-Induced Diabetic Rats.
abstract: We investigated the protective and antioxidative effects of selenium nanoparticles (SeNPs) in streptozotocin STZ-induced diabetic rats. STZ-diabetic rats were exposed daily to treatments with SeNPs and/or insulin and then the effect of these treatments on the parameters correlated to oxidative damage of the rat testes were assessed. Biochemical analysis revealed that SeNPs are able to ameliorate the reduction in the serum testosterone caused by STZ-induced diabetes. Furthermore, SeNPs could significantly decrease testicular tissue oxidative stress markers, namely lipid peroxidation and nitric oxide. In contrast, treatment of the STZ-diabetic rats with SeNPs increased the glutathione content and antioxidant enzyme activities in testicular tissues. Moreover, microscopic analysis proved that SeNPs are able to prevent histological damage in the testes of STZ-diabetic rats. Molecular analysis revealed that the mRNA level ofBcl-2(B-cell lymphoma 2) is significantly upregulated. On the contrary, the mRNA level ofBax(Bcl-2Associated X Protein) was significantly downregulated. Furthermore, treatment of STZ-diabetic rats with SeNPs led to an elevation in the expression of PCNA (Proliferating Cell Nuclear Antigen Gene). Interestingly, the insulin treatment also exhibited a significant improvement in the testicular function in STZ-diabetic rats. Collectively, our results demonstrated the possible effects of SeNPs in attenuating diabetes-induced oxidative damage, in particular in testicular tissue.
authors.affiliation: Department of Zoology, College of Science, King Saud University, Riyadh 11451, Saudi Arabia. mohameddkhil@yahoo.com.|Department of Zoology and Entomology, Faculty of Science, Helwan University, Cairo 11795, Egypt. mohameddkhil@yahoo.com.
authors.forename: Mohamed A
authors.initials: MA
authors.lastname: Dkhil
authors.affiliation: Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, University of Hail, Hail 2440, Saudi Arabia. rafat.zrieq@uni-duesseldorf.de.
authors.forename: Rafat
authors.initials: R
authors.lastname: Zrieq
authors.affiliation: Department of Zoology, College of Science, King Saud University, Riyadh 11451, Saudi Arabia. guraishi@yahoo.com.
authors.forename: Saleh
authors.initials: S
authors.lastname: Al-Quraishy
authors.affiliation: Department of Zoology and Entomology, Faculty of Science, Helwan University, Cairo 11795, Egypt. aest1977@hotmail.com.
authors.forename: Ahmed E
authors.initials: AE
authors.lastname: Abdel Moneim
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage / Q000737:chemistry; D017209:Apoptosis / Q000187:drug effects; D015415:Biomarkers; D003921:Diabetes Mellitus, Experimental; D005978:Glutathione / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D053758:Nanoparticles / Q000737:chemistry; D009569:Nitric Oxide / Q000378:metabolism; D010084:Oxidation-Reduction / Q000187:drug effects; D018384:Oxidative Stress / Q000187:drug effects*; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism; D051381:Rats; D012643:Selenium / Q000008:administration & dosage* / Q000737:chemistry; D013737:Testis / Q000187:drug effects* / Q000473:pathology*
chemical_list: D000975:Antioxidants; D015415:Biomarkers; D018809:Proliferating Cell Nuclear Antigen; D009569:Nitric Oxide; D008315:Malondialdehyde; D005978:Glutathione; D012643:Selenium
keywords: PCNA; apoptosis; diabetes; nanoparticles; oxidative stress; rats; selenium