title: Detection of Mycobacterium avium subsp. paratuberculosis in bovine milk from the state of Pernambuco, Brazil.
abstract: The aim of this study was to detect the IS900 region of Mycobacterium avium subsp. paratuberculosis (MAP) in bovine milk samples using real-time polymerase chain reaction (qPCR) and conventional PCR, and to study the agreement between these tests. A total of 121 bovine milk samples were collected from herds considered positive for MAP, from the State of Pernambuco, Brazil. MAP DNA was detected in 20 samples (16.5%) using conventional PCR and in 34 samples (28.1%) using qPCR. MAP DNA was detected in all of the 6 animal farms studied. Moderate agreement was found between qPCR and conventional PCR results, where the sensitivity and specificity of conventional PCR in relation to qPCR were 50% and 96.6%, respectively. Thus, the IS900 region of MAP was found in bovine milk samples from the State of Pernambuco. To the best of our knowledge, this is the first report of MAP DNA found in bovine milk in Northeast Brazil. We also demonstrated the qPCR technique is more sensitive than conventional PCR with respect to detection of MAP in milk samples.
authors.affiliation: Universidade Rural Federal de Pernambuco, Departamento de Medicina Veterinária, Laboratório de Bacterioses, Recife, PE, Brazil. Electronic address: ppfalbuquerque@gmail.com.
authors.forename: Pedro Paulo Feitosa de
authors.initials: PP
authors.lastname: Albuquerque
authors.affiliation: Universidade Rural Federal de Pernambuco, Departamento de Medicina Veterinária, Laboratório de Bacterioses, Recife, PE, Brazil.
authors.forename: André de Souza
authors.initials: AS
authors.lastname: Santos
authors.affiliation: Universidade Rural Federal de Pernambuco, Departamento de Medicina Veterinária, Laboratório de Bacterioses, Recife, PE, Brazil.
authors.forename: Orestes Luiz de
authors.initials: OL
authors.lastname: Souza Neto
authors.affiliation: Universidade Rural Federal de Pernambuco, Departamento de Medicina Veterinária, Laboratório de Bacterioses, Recife, PE, Brazil.
authors.forename: Pomy de Cássia Peixoto
authors.initials: PC
authors.lastname: Kim
authors.affiliation: Universidade Rural Federal de Pernambuco, Departamento de Medicina Veterinária, Laboratório de Bacterioses, Recife, PE, Brazil.
authors.forename: Erika Fernanda Torres Samico Fernandes
authors.initials: EF
authors.lastname: Cavalcanti
authors.affiliation: Universidade Rural Federal de Pernambuco, Departamento de Medicina Veterinária, Laboratório de Bacterioses, Recife, PE, Brazil.
authors.forename: Júnior Mário Baltazar de
authors.initials: JM
authors.lastname: Oliveira
authors.affiliation: Universidade Rural Federal de Pernambuco, Departamento de Medicina Veterinária, Laboratório de Bacterioses, Recife, PE, Brazil.
authors.forename: Rinaldo Aparecido
authors.initials: RA
authors.lastname: Mota
authors.affiliation: Universidade Rural Federal de Pernambuco, Departamento de Medicina Veterinária, Laboratório de Bacterioses, Recife, PE, Brazil.
authors.forename: José Wilton Pinheiro
authors.initials: JW
authors.lastname: Júnior
mesh_terms: D000818:Animals; D001938:Brazil; D002417:Cattle; D004269:DNA, Bacterial; D005516:Food Microbiology; D008892:Milk / Q000382:microbiology*; D016927:Mycobacterium avium subsp. paratuberculosis / Q000235:genetics* / Q000302:isolation & purification; D010283:Paratuberculosis / Q000175:diagnosis / Q000382:microbiology; D016133:Polymerase Chain Reaction; D012680:Sensitivity and Specificity
chemical_list: D004269:DNA, Bacterial
keywords: Diagnosis; Milk; Molecular biology; Paratuberculosis