title: Identification of transcripts involved in digestion, detoxification and immune response from transcriptome of Empoasca vitis (Hemiptera: Cicadellidae) nymphs.
abstract: Tea production has been significantly impacted by the false-eye leafhopper, Empoasca vitis (Göthe), around Asia. To identify the key genes which are responsible for nutrition absorption, xenobiotic metabolism and immune response, the transcriptome of either alimentary tracts or bodies minus alimentary tract of E. vitis was sequenced and analyzed. Over 31 million reads were obtained from Illumina sequencing. De novo sequence assembly resulted in 52,182 unigenes with a mean size of 848nt. The assembled unigenes were then annotated using various databases. Transcripts of at least 566 digestion-, 224 detoxification-, and 288 immune-related putative genes in E. vitis were identified. In addition, relative expression of highly abundant transcripts was verified through quantitative real-time PCR. Results from this investigation provide genomic information about E. vitis, which will be helpful in further study of E. vitis biology and in the development of novel strategies to control this devastating pest.
authors.affiliation: State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, School of Life Science, Fujian-Taiwan Joint Center for Ecological Control of Crop Pests, Key Lab of Biopesticides and Chemical Biology, MOE, Fujian Agriculture and Forestry University, 350002 Fuzhou, Fujian, PR China; China National Engineering Research Center of JUNCAO Technology, Fujian Agriculture and Forestry University, 350002 Fuzhou, Fujian, PR China.
authors.forename: En-Si
authors.initials: ES
authors.lastname: Shao
authors.affiliation: State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, School of Life Science, Fujian-Taiwan Joint Center for Ecological Control of Crop Pests, Key Lab of Biopesticides and Chemical Biology, MOE, Fujian Agriculture and Forestry University, 350002 Fuzhou, Fujian, PR China.
authors.forename: Gui-Fang
authors.initials: GF
authors.lastname: Lin
authors.affiliation: Department of Entomology, Iowa State University, Ames, Iowa, United States. Electronic address: sliu@iastate.edu.
authors.forename: Sijun
authors.initials: S
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, School of Life Science, Fujian-Taiwan Joint Center for Ecological Control of Crop Pests, Key Lab of Biopesticides and Chemical Biology, MOE, Fujian Agriculture and Forestry University, 350002 Fuzhou, Fujian, PR China.
authors.forename: Xiao-Li
authors.initials: XL
authors.lastname: Ma
authors.affiliation: State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, School of Life Science, Fujian-Taiwan Joint Center for Ecological Control of Crop Pests, Key Lab of Biopesticides and Chemical Biology, MOE, Fujian Agriculture and Forestry University, 350002 Fuzhou, Fujian, PR China.
authors.forename: Ming-Feng
authors.initials: MF
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, School of Life Science, Fujian-Taiwan Joint Center for Ecological Control of Crop Pests, Key Lab of Biopesticides and Chemical Biology, MOE, Fujian Agriculture and Forestry University, 350002 Fuzhou, Fujian, PR China.
authors.forename: Li
authors.initials: L
authors.lastname: Lin
authors.affiliation: State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, School of Life Science, Fujian-Taiwan Joint Center for Ecological Control of Crop Pests, Key Lab of Biopesticides and Chemical Biology, MOE, Fujian Agriculture and Forestry University, 350002 Fuzhou, Fujian, PR China.
authors.forename: Song-Qing
authors.initials: SQ
authors.lastname: Wu
authors.affiliation: State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, School of Life Science, Fujian-Taiwan Joint Center for Ecological Control of Crop Pests, Key Lab of Biopesticides and Chemical Biology, MOE, Fujian Agriculture and Forestry University, 350002 Fuzhou, Fujian, PR China.
authors.forename: Li
authors.initials: L
authors.lastname: Sha
authors.affiliation: State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, School of Life Science, Fujian-Taiwan Joint Center for Ecological Control of Crop Pests, Key Lab of Biopesticides and Chemical Biology, MOE, Fujian Agriculture and Forestry University, 350002 Fuzhou, Fujian, PR China.
authors.forename: Zhao-Xia
authors.initials: ZX
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, School of Life Science, Fujian-Taiwan Joint Center for Ecological Control of Crop Pests, Key Lab of Biopesticides and Chemical Biology, MOE, Fujian Agriculture and Forestry University, 350002 Fuzhou, Fujian, PR China.
authors.forename: Xiao-Hua
authors.initials: XH
authors.lastname: Hu
authors.affiliation: State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, School of Life Science, Fujian-Taiwan Joint Center for Ecological Control of Crop Pests, Key Lab of Biopesticides and Chemical Biology, MOE, Fujian Agriculture and Forestry University, 350002 Fuzhou, Fujian, PR China.
authors.forename: Xiong
authors.initials: X
authors.lastname: Guan
authors.affiliation: State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, School of Life Science, Fujian-Taiwan Joint Center for Ecological Control of Crop Pests, Key Lab of Biopesticides and Chemical Biology, MOE, Fujian Agriculture and Forestry University, 350002 Fuzhou, Fujian, PR China. Electronic address: lingling0264@163.com.
authors.forename: Ling-Ling
authors.initials: LL
authors.lastname: Zhang
mesh_terms: D000818:Animals; D004063:Digestion / Q000235:genetics*; D006430:Hemiptera / Q000235:genetics* / Q000276:immunology / Q000378:metabolism / Q000502:physiology; D007107:Immune System; D008658:Inactivation, Metabolic / Q000235:genetics*; D009758:Nymph / Q000235:genetics; D059467:Transcriptome
keywords: Alimentary tract; Detoxification; Digestion; Empoasca vitis; Illumina sequencing; Immunity