title: Growth and wax ester production of an Acinetobacter baylyi ADP1 mutant deficient in exopolysaccharide capsule synthesis.
abstract: Acinetobacter baylyi ADP1 naturally produces wax esters that could be used as a raw material in industrial applications. We attempted to improve wax ester yield of A. baylyi ADP1 by removing rmlA, a gene involved in exopolysaccharide production. Growth rate, biomass formation and wax ester yield on 4-hydroxybenzoate were not affected, but the rmlA-strain grew slower on acetate, while reaching similar biomass and wax ester yield. The rmlA-cells had malformed shape and large size and grew poorly on glucose without expression of the gene for pyruvate kinase (pykF) from Escherichia coli. The pykF-expressing rmlA-strain had similar growth rate, lowered biomass formation and improved wax ester production on glucose as compared to the wild-type strain expressing pykF. Cultivation of the pykF-expressing rmlA-strain on an elevated glucose concentration in a medium supplemented with amino acids resulted in doubled molar wax ester yield and acetate production.
authors.affiliation: Department of Chemistry and Bioengineering, Tampere University of Technology, Korkeakoulunkatu 8, Tampere, Finland. matti.kannisto@tut.fi.
authors.forename: Matti
authors.initials: M
authors.lastname: Kannisto
authors.affiliation: Department of Chemistry and Bioengineering, Tampere University of Technology, Korkeakoulunkatu 8, Tampere, Finland.
authors.forename: Elena
authors.initials: E
authors.lastname: Efimova
authors.affiliation: Department of Chemistry and Bioengineering, Tampere University of Technology, Korkeakoulunkatu 8, Tampere, Finland.
authors.forename: Matti
authors.initials: M
authors.lastname: Karp
authors.affiliation: Department of Chemistry and Bioengineering, Tampere University of Technology, Korkeakoulunkatu 8, Tampere, Finland.
authors.forename: Ville
authors.initials: V
authors.lastname: Santala
mesh_terms: D000085:Acetates / Q000737:chemistry; D000150:Acinetobacter / Q000235:genetics* / Q000378:metabolism*; D018533:Biomass; D004926:Escherichia coli / Q000201:enzymology; D004952:Esters / Q000378:metabolism*; D007218:Industrial Microbiology; D009713:Nucleotidyltransferases / Q000235:genetics*; D010226:Parabens / Q000737:chemistry*; D011770:Pyruvate Kinase
chemical_list: D000085:Acetates; D004952:Esters; D010226:Parabens; D011770:Pyruvate Kinase; D009713:Nucleotidyltransferases; C020587:glucose-1-phosphate thymidylyltransferase; C038193:4-hydroxybenzoic acid
keywords: Acinetobacter baylyi; Exopolysaccharide; Metabolic engineering; Overflow metabolism; Wax ester