title: Liquid chromatographic/electrospray ionization quadrupole/time of flight tandem mass spectrometric study of polyphenolic composition of different Vaccinium berry species and their comparative evaluation.
abstract: Ultra-high-performance liquid chromatography coupled with high-resolution quadrupole-time of flight mass spectrometry with both negative and positive ionization was used for comprehensively investigating the phenolic and polyphenolic compounds in berries from three spontaneous or cultivated Vaccinium species (i.e., Vaccinium myrtillus, Vaccinium uliginosum subsp. gaultherioides, and Vaccinium corymbosum). More than 200 analytes, among phenolic and polyphenolic compounds belonging to the classes of anthocyanins, monomeric and oligomeric flavonols, flavanols, dihydrochalcones, phenolic acids, together with other polyphenolic compounds of mixed structural characteristics, were identified. Some of the polyphenols herein investigated, such as anthocyanidin glucuronides and malvidin-feruloyl-hexosides in V. myrtillus, or anthocyanindin aldopentosides and coumaroyl-hexosides in V. uliginosum subsp. gaultherioides and a large number of proanthocyanidins with high molecular weight in all species, were described for the first time in these berries. Principal component analysis applied on original LC-TOF data, acquired in survey scan mode, successfully discriminated the three Vaccinium berry species investigated, on the basis of their polyphenolic composition, underlying one more time the fundamental role of mass spectrometry for food characterization.
authors.affiliation: Department of Chemistry, University of Florence, Via della Lastruccia 3, 50019, Sesto Fiorentino, Florence, Italy.
authors.forename: Claudia
authors.initials: C
authors.lastname: Ancillotti
authors.affiliation: Department of Chemistry, University of Florence, Via della Lastruccia 3, 50019, Sesto Fiorentino, Florence, Italy.
authors.forename: Lorenzo
authors.initials: L
authors.lastname: Ciofi
authors.affiliation: Department of Chemistry, University of Florence, Via della Lastruccia 3, 50019, Sesto Fiorentino, Florence, Italy.
authors.forename: Daniele
authors.initials: D
authors.lastname: Rossini
authors.affiliation: Sciex Europe, Landwehrstraße 54, 64293, Darmstadt, Germany.
authors.forename: Ugo
authors.initials: U
authors.lastname: Chiuminatto
authors.affiliation: Sciex Europe, Landwehrstraße 54, 64293, Darmstadt, Germany.
authors.forename: Jianru
authors.initials: J
authors.lastname: Stahl-Zeng
authors.affiliation: Department of Chemistry, University of Florence, Via della Lastruccia 3, 50019, Sesto Fiorentino, Florence, Italy.
authors.forename: Serena
authors.initials: S
authors.lastname: Orlandini
authors.affiliation: Department of Chemistry, University of Florence, Via della Lastruccia 3, 50019, Sesto Fiorentino, Florence, Italy.
authors.forename: Sandra
authors.initials: S
authors.lastname: Furlanetto
authors.affiliation: Department of Chemistry, University of Florence, Via della Lastruccia 3, 50019, Sesto Fiorentino, Florence, Italy. delbubba@unifi.it.
authors.forename: Massimo
authors.initials: M
authors.lastname: Del Bubba
mesh_terms: D002853:Chromatography, Liquid / Q000379:methods*; D059808:Polyphenols / Q000032:analysis*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D053719:Tandem Mass Spectrometry / Q000379:methods*; D029796:Vaccinium / Q000737:chemistry*
chemical_list: D059808:Polyphenols
keywords: Flavonoids; High-resolution mass spectrometry; Liquid chromatography; Polyphenols; Principal component analysis; Vaccinium species