title: A platform for the development of novel biosensors by configuring allosteric transcription factor recognition with amplified luminescent proximity homogeneous assays.
abstract: A wide range of chemicals can be sensed by allosteric transcription factors (aTFs) in bacteria. Herein, we report a biosensing platform by using isolated aTFs as recognition elements in vitro. Moreover, a general strategy to increase the sensitivity of the aTF-based biosensors is provided. As a proof-of-concept, we obtained by far the most sensitive uric acid and oxytetracycline biosensors by using aTF HucR and OtrR as recognition elements, respectively. As a large number of aTFs are present in bacteria, our work opens a novel route to develop sensitive aTF-based biosensors.
authors.affiliation: State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, 100101 Beijing, China. yangkq@im.ac.cn wangws@im.ac.cn.
authors.forename: Shanshan
authors.initials: S
authors.lastname: Li
authors.affiliation: Institute of Health Sciences, Anhui University, 230601, Hefei, China.
authors.forename: Li
authors.initials: L
authors.lastname: Zhou
authors.affiliation: State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, 100101 Beijing, China. yangkq@im.ac.cn wangws@im.ac.cn.
authors.forename: Yongpeng
authors.initials: Y
authors.lastname: Yao
authors.affiliation: State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, 100101 Beijing, China. yangkq@im.ac.cn wangws@im.ac.cn.
authors.forename: Keqiang
authors.identifier: 0000-0003-2420-1889
authors.initials: K
authors.lastname: Fan
authors.affiliation: State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, 100101 Beijing, China. yangkq@im.ac.cn wangws@im.ac.cn.
authors.forename: Zilong
authors.initials: Z
authors.lastname: Li
authors.affiliation: State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, 100101 Beijing, China. yangkq@im.ac.cn wangws@im.ac.cn and Institute of Health Sciences, Anhui University, 230601, Hefei, China.
authors.forename: Lixin
authors.initials: L
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, 100101 Beijing, China. yangkq@im.ac.cn wangws@im.ac.cn.
authors.forename: Weishan
authors.initials: W
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, 100101 Beijing, China. yangkq@im.ac.cn wangws@im.ac.cn.
authors.forename: Keqian
authors.initials: K
authors.lastname: Yang
mesh_terms: D000494:Allosteric Regulation; D001483:Base Sequence; D015374:Biosensing Techniques / Q000379:methods*; D004247:DNA / Q000235:genetics / Q000378:metabolism; D008163:Luminescent Measurements / Q000379:methods*; D010118:Oxytetracycline / Q000032:analysis; D014157:Transcription Factors / Q000737:chemistry* / Q000378:metabolism; D014527:Uric Acid / Q000032:analysis
chemical_list: D014157:Transcription Factors; D014527:Uric Acid; D004247:DNA; D010118:Oxytetracycline