title: Single cell screening approaches for antibody discovery.
abstract: Microtools that have been developed to allow in depth interrogation of individual cells in high throughput are improving our understanding of biological processes at the single cell level and are opening up new possibilities for biological research. In relation to antibody discovery, these tools are now helping to maximise the full potential of well-established methodologies for antibody generation. Being complementary to both recombinant and native antibody secreting cells, some of these tools are finding widespread use in the field. In this review, we discuss how microtools for single cell analysis are addressing some of the limitations of traditional approaches for antibody screening. We describe the main classes of microtools for antibody discovery along with a comparison of each technology and an outlook for the future utility of some of these microtools for discovery and research.
authors.affiliation: Vaccinogen Inc., 949 Fell Street, 2nd Floor, Baltimore, MD 21231, United States.
authors.forename: Valerie
authors.initials: V
authors.lastname: Fitzgerald
authors.affiliation: Vaccinogen Inc., 949 Fell Street, 2nd Floor, Baltimore, MD 21231, United States. Electronic address: pleonard@vaccinogeninc.com.
authors.forename: Paul
authors.initials: P
authors.lastname: Leonard
mesh_terms: D000818:Animals; D000906:Antibodies / Q000737:chemistry / Q000302:isolation & purification*; D000917:Antibody Formation; D055808:Drug Discovery; D005434:Flow Cytometry / Q000295:instrumentation*; D057166:High-Throughput Screening Assays / Q000295:instrumentation / Q000379:methods*; D006801:Humans; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*; D059010:Single-Cell Analysis / Q000295:instrumentation / Q000379:methods*
chemical_list: D000906:Antibodies
keywords: Antibody screening; Microtool; Single cell analysis