title: Paper-based magnetic nanoparticle-peptide probe for rapid and quantitative colorimetric detection of Escherichia coli O157:H7.
abstract: There is a critical and urgent demand for a simple, rapid and specific qualitative and quantitative colorimetric biosensor for the detection of the food contaminant Escherichia coli O157:H7 (E. coli O157:H7) in complex food products due to the recent outbreaks of food-borne diseases. Traditional detection techniques are time-consuming, require expensive instrumentation and are labour-intensive. To overcome these limitations, a novel, ultra-rapid visual biosensor was developed based on the ability of E. coli O157:H7 proteases to change the optical response of a surface-modified, magnetic nanoparticle-specific (MNP-specific) peptide probe. Upon proteolysis, a gradual increase in the golden color of the sensor surface was visually observed. The intensification of color was correlated with the E. coli O157:H7 concentration. The color change resulting from the dissociation of the self-assembled monolayer (SAM) was detected by the naked eye and analysed using an image analysis software (ImageJ) for the purpose of quantitative detection. This biosensor demonstrated high sensitivity and applicability, with lower limits of detection of 12CFUmL-1in broth samples and 30-300CFUmL-1in spiked complex food matrices. In conclusion, this approach permits the use of a disposable biosensor chip that can be mass-produced at low cost and can be used not only by food manufacturers but also by regulatory agencies for better control of potential health risks associated with the consumption of contaminated foods.
authors.affiliation: Department of Pharmaceutical Sciences, Faculty of Pharmacy, The University of Jordan, Amman 11942, Jordan.
authors.forename: Ghadeer A R Y
authors.initials: GARY
authors.lastname: Suaifan
authors.affiliation: Department of Chemistry, Alfaisal University, Al Zahrawi Street, Al Maather, Al Takhassusi Rd, Riyadh 11533, Saudi Arabia.
authors.forename: Sahar
authors.initials: S
authors.lastname: Alhogail
authors.affiliation: Department of Chemistry, Alfaisal University, Al Zahrawi Street, Al Maather, Al Takhassusi Rd, Riyadh 11533, Saudi Arabia; King Faisal Specialist Hospital and Research Center, Zahrawi Street, Al Maather, Riyadh 12713, Saudi Arabia. Electronic address: mzourob@alfaisal.edu.
authors.forename: Mohammed
authors.initials: M
authors.lastname: Zourob
mesh_terms: D000818:Animals; D015374:Biosensing Techniques / Q000379:methods*; D002417:Cattle; D003124:Colorimetry / Q000379:methods; D004927:Escherichia coli Infections / Q000382:microbiology; D019453:Escherichia coli O157 / Q000201:enzymology / Q000302:isolation & purification*; D005506:Food Contamination / Q000032:analysis*; D005516:Food Microbiology; D005517:Foodborne Diseases / Q000382:microbiology; D006801:Humans; D018545:Lactuca / Q000382:microbiology; D057230:Limit of Detection; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D008460:Meat / Q000032:analysis; D008892:Milk / Q000382:microbiology; D010447:Peptide Hydrolases / Q000737:chemistry; D010455:Peptides / Q000737:chemistry*; D014421:Turkey
chemical_list: D058185:Magnetite Nanoparticles; D010455:Peptides; D010447:Peptide Hydrolases
keywords: Colorimetric biosensor; Escherichia coli O157:H7; Food-borne illness; Magnetic-nano particles