title: Mesenchymal Stem Cell Magnetization: Magnetic Multilayer Microcapsule Uptake, Toxicity, Impact on Functional Properties, and Perspectives for Magnetic Delivery.
abstract: Mesenchymal stem cells (MSCs) are widely used in cell therapy due to their convenience, multiline differentiation potential, reproducible protocols, and biological properties. The potential of MSCs to impregnate magnetic microcapsules and their possible influence on cell function and ability to response to magnetic field have been explored. Interestingly, the cells suspended in media show much higher ability in internalization of microcapsules, then MSCs adhere into the surface. There is no significant effect of microcapsules on cell toxicity compared with other cell line-capsule internalization reported in literature. Due to internalization of magnetic capsules by the cells, such cell engineering platform is responsive to external magnetic field, which allows to manipulate MSC migration. Magnetically sorted MSCs are capable to differentiation as confirmed by their conversion to adipogenic and osteogenic cells using standard protocols. There is a minor effect of capsule internalization on cell adhesion, though MSCs are still able to form spheroid made by dozen of thousand MSCs. This work demonstrates the potential of use of microcapsule impregnated MSCs to carry internalized micron-sized vesicles and being navigated with external magnetic signaling.
authors.affiliation: Department of Hematology, Transfusion, and Transplantation, First I. P. Pavlov State Medical University of St. Petersburg, Lev Tolstoy str., 6/8, 197022, Saint Petersburg, Russian Federation.
authors.forename: Kirill V
authors.initials: KV
authors.lastname: Lepik
authors.affiliation: Department of Hematology, Transfusion, and Transplantation, First I. P. Pavlov State Medical University of St. Petersburg, Lev Tolstoy str., 6/8, 197022, Saint Petersburg, Russian Federation.
authors.forename: Albert R
authors.initials: AR
authors.lastname: Muslimov
authors.affiliation: RASA Center in Tomsk, Tomsk Polytechnic University, pros. Lenina, 30, 634050, Tomsk, Russian Federation.
authors.forename: Alexander S
authors.initials: AS
authors.lastname: Timin
authors.affiliation: Department of Hematology, Transfusion, and Transplantation, First I. P. Pavlov State Medical University of St. Petersburg, Lev Tolstoy str., 6/8, 197022, Saint Petersburg, Russian Federation.
authors.forename: Vladislav S
authors.initials: VS
authors.lastname: Sergeev
authors.affiliation: Department of Hematology, Transfusion, and Transplantation, First I. P. Pavlov State Medical University of St. Petersburg, Lev Tolstoy str., 6/8, 197022, Saint Petersburg, Russian Federation.
authors.forename: Dmitry S
authors.initials: DS
authors.lastname: Romanyuk
authors.affiliation: Department of Hematology, Transfusion, and Transplantation, First I. P. Pavlov State Medical University of St. Petersburg, Lev Tolstoy str., 6/8, 197022, Saint Petersburg, Russian Federation.
authors.forename: Ivan S
authors.initials: IS
authors.lastname: Moiseev
authors.affiliation: RASA Center in St. Petersburg, Peter The Great St. Petersburg Polytechnic University, Polytechnicheskaya, 29, 195251, Saint Petersburg, Russian Federation.|Institute of Macromolecular Compounds, Russian Academy of Sciences, Birzhevoy proezd str. 6, 199004, Saint Petersburg, Russian Federation.
authors.forename: Elena V
authors.initials: EV
authors.lastname: Popova
authors.affiliation: RASA Center in St. Petersburg, Peter The Great St. Petersburg Polytechnic University, Polytechnicheskaya, 29, 195251, Saint Petersburg, Russian Federation.
authors.forename: Igor L
authors.initials: IL
authors.lastname: Radchenko
authors.affiliation: RASA Center in St. Petersburg, Peter The Great St. Petersburg Polytechnic University, Polytechnicheskaya, 29, 195251, Saint Petersburg, Russian Federation.|Institute of Macromolecular Compounds, Russian Academy of Sciences, Birzhevoy proezd str. 6, 199004, Saint Petersburg, Russian Federation.
authors.forename: Alexander D
authors.initials: AD
authors.lastname: Vilesov
authors.affiliation: Department of Hematology, Transfusion, and Transplantation, First I. P. Pavlov State Medical University of St. Petersburg, Lev Tolstoy str., 6/8, 197022, Saint Petersburg, Russian Federation.
authors.forename: Oleg V
authors.initials: OV
authors.lastname: Galibin
authors.affiliation: RASA Center in St. Petersburg, Peter The Great St. Petersburg Polytechnic University, Polytechnicheskaya, 29, 195251, Saint Petersburg, Russian Federation.|School of Engineering and Materials Science, Queen Mary University of London, Mile End Road, London, E1 4NS, UK.
authors.forename: Gleb B
authors.initials: GB
authors.lastname: Sukhorukov
authors.affiliation: Department of Hematology, Transfusion, and Transplantation, First I. P. Pavlov State Medical University of St. Petersburg, Lev Tolstoy str., 6/8, 197022, Saint Petersburg, Russian Federation.
authors.forename: Boris V
authors.initials: BV
authors.lastname: Afanasyev
mesh_terms: D050156:Adipogenesis / Q000187:drug effects; D002214:Capsules / Q000008:administration & dosage* / Q000009:adverse effects*; D002448:Cell Adhesion / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects; D002465:Cell Movement / Q000187:drug effects; D064987:Cell- and Tissue-Based Therapy / Q000379:methods; D002478:Cells, Cultured; D006801:Humans; D060526:Magnetic Fields / Q000009:adverse effects; D008280:Magnetics / Q000379:methods; D059630:Mesenchymal Stem Cells / Q000187:drug effects* / Q000378:metabolism*; D010012:Osteogenesis / Q000187:drug effects; D015398:Signal Transduction / Q000187:drug effects
chemical_list: D002214:Capsules
keywords: cell delivery; cell therapy; magnetic delivery; polyelectrolyte capsules; stem cells