title: Characterization of a common wheat (Triticum aestivum L.) high-tillering dwarf mutant.
abstract: A novel high-tillering dwarf mutant in common wheat Wangshuibai was characterized and mapped to facilitate breeding for plant height and tiller and the future cloning of the causal gene. Tiller number and plant height are two major agronomic traits in cereal crops affecting plant architecture and grain yield. NAUH167, a mutant of common wheat landrace Wangshuibai induced by ethylmethyl sulfide (EMS) treatment, exhibits higher tiller number and reduced plant height. Microscope observation showed that the dwarf phenotype was attributed to the decrease in the number of cells and their length. The same as the wild type, the mutant was sensitive to exogenous gibberellins. Genetic analysis showed that the high-tillering number and dwarf phenotype were related and controlled by a partial recessive gene. Using a RIL2:6population derived from the cross NAUH167/Sumai3, a molecular marker-based genetic map was constructed. The map consisted of 283 loci, spanning a total length of 1007.98 cM with an average markers interval of 3.56 cM. By composite interval mapping, a stable major QTL designated QHt.nau-2D controlling both traits, was mapped to the short arm of chromosome 2D flanked by markers Xcfd11 and Xgpw361. To further map the QHt.nau-2D loci, another population consisted of 180 F2progeny from a cross 2011I-78/NAUH167 was constructed. Finally, QHt.nau-2D was located within a genetic region of 0.8 cM between markers QHT239 and QHT187 covering a predicted physical distance of 6.77 Mb. This research laid the foundation for map-based cloning of QHt.nau-2D and would facilitate the characterization of plant height and tiller number in wheat.
authors.affiliation: State Key Laboratory of Crop Genetics and Germplasm Enhancement, Cytogenetics Institute, Nanjing Agricultural University/JCIC-MCP, Nanjing, 210095, Jiangsu, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Xu
authors.affiliation: State Key Laboratory of Crop Genetics and Germplasm Enhancement, Cytogenetics Institute, Nanjing Agricultural University/JCIC-MCP, Nanjing, 210095, Jiangsu, China.
authors.forename: Nengfei
authors.initials: N
authors.lastname: Bian
authors.affiliation: State Key Laboratory of Crop Genetics and Germplasm Enhancement, Cytogenetics Institute, Nanjing Agricultural University/JCIC-MCP, Nanjing, 210095, Jiangsu, China.
authors.forename: Mingxing
authors.initials: M
authors.lastname: Wen
authors.affiliation: State Key Laboratory of Crop Genetics and Germplasm Enhancement, Cytogenetics Institute, Nanjing Agricultural University/JCIC-MCP, Nanjing, 210095, Jiangsu, China.
authors.forename: Jin
authors.initials: J
authors.lastname: Xiao
authors.affiliation: State Key Laboratory of Crop Genetics and Germplasm Enhancement, Cytogenetics Institute, Nanjing Agricultural University/JCIC-MCP, Nanjing, 210095, Jiangsu, China.
authors.forename: Chunxia
authors.initials: C
authors.lastname: Yuan
authors.affiliation: State Key Laboratory of Crop Genetics and Germplasm Enhancement, Cytogenetics Institute, Nanjing Agricultural University/JCIC-MCP, Nanjing, 210095, Jiangsu, China.
authors.forename: Aizhong
authors.initials: A
authors.lastname: Cao
authors.affiliation: State Key Laboratory of Crop Genetics and Germplasm Enhancement, Cytogenetics Institute, Nanjing Agricultural University/JCIC-MCP, Nanjing, 210095, Jiangsu, China.
authors.forename: Shouzhong
authors.initials: S
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Crop Genetics and Germplasm Enhancement, Cytogenetics Institute, Nanjing Agricultural University/JCIC-MCP, Nanjing, 210095, Jiangsu, China. xiuew@njau.edu.cn.
authors.forename: Xiue
authors.initials: X
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Crop Genetics and Germplasm Enhancement, Cytogenetics Institute, Nanjing Agricultural University/JCIC-MCP, Nanjing, 210095, Jiangsu, China. hywang@njau.edu.cn.
authors.forename: Haiyan
authors.initials: H
authors.lastname: Wang
mesh_terms: D002874:Chromosome Mapping; D004252:DNA Mutational Analysis; D018744:DNA, Plant / Q000235:genetics; D005808:Genes, Recessive; D008040:Genetic Linkage; D005819:Genetic Markers; D005875:Gibberellins / Q000737:chemistry; D018895:Microsatellite Repeats; D016296:Mutagenesis; D010641:Phenotype; D000069600:Plant Breeding; D040641:Quantitative Trait Loci; D014908:Triticum / Q000235:genetics* / Q000254:growth & development*
chemical_list: D018744:DNA, Plant; D005819:Genetic Markers; D005875:Gibberellins; C007842:gibberellic acid