title: Depicting the Discrepancy between Tri Genotype and Chemotype on the Basis of Strain CBS 139514 from a Field Population of F. graminearum Sensu Stricto from Argentina.
abstract: Recent studies on a field population ofF. graminearumsensu stricto from Argentina revealed an atypical panel of strains identified through PCR genotyping as 15ADON genotypes, but producing high levels of 3ADON. Based on representative strain CBS 139514, we asked if the discrepancy between the trichothecene genotype and chemotype might result from an inter-chemotype recombination of the chemotype-determining genes. To answer this, we sequenced the complete coreTrigene cluster (around 30,200 bp) from this strain and compared its sequence to sequence data of typical type B trichothecene genotypes/chemotypes. Sequence alignment showed that CBS 139514 has an identical sequence within the entire coreTricluster to the 15ADON genotype. The revealed discrepancy underlines the need for using both molecular and chemical methods for reliable characterization of toxigenic strains ofFusarium.
authors.affiliation: Department of Botany and Nature Protection, University of Warmia and Mazury in Olsztyn, Plac Łódzki 1, Olsztyn 10-727, Poland. tomaszkulik76@gmail.com.
authors.forename: Tomasz
authors.initials: T
authors.lastname: Kulik
authors.affiliation: Department of Chemistry, Poznań University of Life Sciences, ul. Wojska Polskiego 75, Poznań 60-625, Poland. mabu@au.poznan.pl.
authors.forename: Maciej
authors.initials: M
authors.lastname: Buśko
authors.affiliation: Department of Botany and Nature Protection, University of Warmia and Mazury in Olsztyn, Plac Łódzki 1, Olsztyn 10-727, Poland. katarzyna.wolosz@uwm.edu.pl.
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Bilska
authors.affiliation: Department of Chemistry, Poznań University of Life Sciences, ul. Wojska Polskiego 75, Poznań 60-625, Poland. ostrowska.anna.maria@gmail.com.
authors.forename: Anna
authors.initials: A
authors.lastname: Ostrowska-Kołodziejczak
authors.affiliation: CBS-KNAW Fungal Biodiversity Centre, Uppsalalaan 8, Utrecht 3584 CT, The Netherlands. a.diepeningen@cbs.knaw.nl.|Institute of Biodiversity and Ecosystem Dynamics, University of Amsterdam, Science Park 904, Amsterdam 1098 XH, The Netherlands. a.diepeningen@cbs.knaw.nl.
authors.forename: Anne D
authors.initials: AD
authors.lastname: van Diepeningen
authors.affiliation: Department of Chemistry, Poznań University of Life Sciences, ul. Wojska Polskiego 75, Poznań 60-625, Poland. julperk@au.poznan.pl.
authors.forename: Juliusz
authors.initials: J
authors.lastname: Perkowski
authors.affiliation: Laboratorio de Biología Funcional y Biotecnología (BIOLAB)-Comisión de Investigaciones Científicas de la provincia de Buenos Aires-Instituto de Investigaciones en Biodiversidad y Biotecnología-Consejo Nacional de Investigaciones Científicas y Técnicas (CICBA-INBIOTEC, CONICET), Av. República de Italia 780, Azul 7300, Buenos Aires, Argentina. stenglein@faa.unicen.edu.ar.|Cátedra de Microbiología-Facultad de Agronomía de Azul-UNCPBA, Av. República de Italia 780, Azul 7300, Buenos Aires, Argentina. stenglein@faa.unicen.edu.ar.
authors.forename: Sebastian
authors.initials: S
authors.lastname: Stenglein
mesh_terms: D001118:Argentina; D004271:DNA, Fungal / Q000032:analysis; D005670:Fusarium / Q000235:genetics* / Q000378:metabolism*; D005800:Genes, Fungal; D005838:Genotype; D014255:Trichothecenes / Q000096:biosynthesis* / Q000235:genetics*
chemical_list: D004271:DNA, Fungal; D014255:Trichothecenes
keywords: Fusarium; Tri genotypes; chemotypes