title: Co-delivery of zinc and 5-aminosalicylic acid from alginate/N-succinyl-chitosan blend microspheres for synergistic therapy of colitis.
abstract: The present study developed novel zinc ion cross-linked alginate/N-succinyl-chitosan (NSC) blend microspheres (MS) for co-delivery of zinc and 5-aminosalicylic acid (5-ASA) for synergistic therapy of colitis. Physicochemical characterization of blend MS was assessed using scanning electron microscopy (SEM), Fourier transform infrared (FTIR) spectroscopy, and energy dispersive X-ray spectrometer (EDS). In vitro release studies demonstrated that blend MS has a pH-dependent release property. Both 5-ASA and zinc have lower release in acid medium and higher release in colonic environment. The therapeutic efficacy of zinc cross-linked blend MS was evaluated using induced-colitis rat models, and showed a superior treatment effect in alleviating inflammation of colitis rats. No systemic toxicity was observed after oral administration of blend MS. Therefore, zinc ion cross-linked alginate/N-succinyl-chitosan blend MS appeared to be a good candidate for co-delivery of zinc and 5-ASA to colon, and had great potential application in inflammatory bowel diseases (IBD) treatment.
authors.affiliation: Department of Chemistry, Lanzhou University, Lanzhou 730000, PR China; Department of Pharmacy, The First Hospital of Lanzhou University, Lanzhou 730000, PR China. Electronic address: duanhg13@lzu.edu.cn.
authors.forename: Haogang
authors.initials: H
authors.lastname: Duan
authors.affiliation: Department of Chemistry, Lanzhou University, Lanzhou 730000, PR China. Electronic address: lshy@lzu.edu.cn.
authors.forename: Shaoyu
authors.initials: S
authors.lastname: Lü
authors.affiliation: Department of Pharmacy, The First Hospital of Lanzhou University, Lanzhou 730000, PR China. Electronic address: candyqinhy@163.com.
authors.forename: Hongyan
authors.initials: H
authors.lastname: Qin
authors.affiliation: Department of Chemistry, Lanzhou University, Lanzhou 730000, PR China. Electronic address: gaochm@lzu.edu.cn.
authors.forename: Chunmei
authors.initials: C
authors.lastname: Gao
authors.affiliation: Department of Chemistry, Lanzhou University, Lanzhou 730000, PR China. Electronic address: baix12@lzu.edu.cn.
authors.forename: Xiao
authors.initials: X
authors.lastname: Bai
authors.affiliation: Department of Pharmacy, The First Hospital of Lanzhou University, Lanzhou 730000, PR China. Electronic address: weiyh_001@163.com.
authors.forename: Yuhui
authors.initials: Y
authors.lastname: Wei
authors.affiliation: Department of Pharmacy, The First Hospital of Lanzhou University, Lanzhou 730000, PR China. Electronic address: xinanwu6511@163.com.
authors.forename: Xin'an
authors.initials: X
authors.lastname: Wu
authors.affiliation: Department of Chemistry, Lanzhou University, Lanzhou 730000, PR China. Electronic address: mzliu@lzu.edu.cn.
authors.forename: Mingzhu
authors.initials: M
authors.lastname: Liu
authors.affiliation: Department of Chemical Engineering, Auburn University, Auburn, AL, 36849, USA. Electronic address: xzz0004@auburn.edu.
authors.forename: Xinyu
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Department of Physics and Engineering, Frostburg State University, Frostburg, MD 21532-2303, USA. Electronic address: zliu@frostburg.edu.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Liu
mesh_terms: D000284:Administration, Oral; D000464:Alginates / Q000737:chemistry; D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000008:administration & dosage / Q000494:pharmacology; D048271:Chitosan / Q000737:chemistry; D003092:Colitis / Q000188:drug therapy* / Q000473:pathology; D004195:Disease Models, Animal; D004337:Drug Carriers / Q000737:chemistry; D004338:Drug Combinations; D016503:Drug Delivery Systems; D065546:Drug Liberation; D004357:Drug Synergism; D020723:Glucuronic Acid / Q000737:chemistry; D006603:Hexuronic Acids / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D008297:Male; D019804:Mesalamine / Q000008:administration & dosage* / Q000494:pharmacology; D008855:Microscopy, Electron, Scanning; D008863:Microspheres; D051381:Rats; D017208:Rats, Wistar; D017550:Spectroscopy, Fourier Transform Infrared; D015032:Zinc / Q000008:administration & dosage* / Q000494:pharmacology
chemical_list: D000464:Alginates; D000894:Anti-Inflammatory Agents, Non-Steroidal; D004337:Drug Carriers; D004338:Drug Combinations; D006603:Hexuronic Acids; C118639:N-succinyl-chitosan; D019804:Mesalamine; D020723:Glucuronic Acid; D048271:Chitosan; D015032:Zinc
keywords: Alginate; Blend microspheres; Co-delivery; Colon-specific; N-succinyl-chitosan