title: MicroRNAs for osteosarcoma in the mouse: a meta-analysis.
abstract: Osteosarcoma (OS) is the most common primary malignant bone carcinoma with high morbidity that happens mainly in children and young adults. As the key components of gene-regulatory networks, microRNAs (miRNAs) control many critical pathophysiological processes, including initiation and progression of cancers. The objective of this study is to summarize and evaluate the potential of miRNAs as targets for prevention and treatment of OS in mouse models, and to explore the methodological quality of current studies. We searched PubMed, Web of Science, Embase, Wan Fang Database, VIP Database, China Knowledge Resource Integrated Database, and Chinese BioMedical since their beginning date to 10 May 2016. Two reviewers separately screened the controlled studies, which estimate the effects of miRNAs on osteosarcoma in mice. A pair-wise analysis was performed. Thirty six studies with enough randomization were selected and included in the meta-analysis. We found that blocking oncogenic or restoring decreased miRNAs in cancer cells could significantly suppress the progression of OS in vivo, as assessed by tumor volume and tumor weight. This meta-analysis suggests that miRNAs are potential therapeutic targets for OS and correction of the altered expression of miRNAs significantly suppresses the progression of OS in mouse models, however, the overall methodological quality of studies included here was low, and more animal studies with the rigourous design must be carried out before a miRNA-based treatment could be translated from animal studies to clinical trials.
authors.affiliation: Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, China.|Spine Institute, Shanghai University of Traditional Chinese Medicine, Shanghai, China.
authors.forename: Junli
authors.initials: J
authors.lastname: Chang
authors.affiliation: Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, China.|Spine Institute, Shanghai University of Traditional Chinese Medicine, Shanghai, China.
authors.forename: Min
authors.initials: M
authors.lastname: Yao
authors.affiliation: Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, China.|Spine Institute, Shanghai University of Traditional Chinese Medicine, Shanghai, China.
authors.forename: Yimian
authors.initials: Y
authors.lastname: Li
authors.affiliation: Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, China.|Spine Institute, Shanghai University of Traditional Chinese Medicine, Shanghai, China.
authors.forename: Dongfeng
authors.initials: D
authors.lastname: Zhao
authors.affiliation: Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, China.|Spine Institute, Shanghai University of Traditional Chinese Medicine, Shanghai, China.
authors.forename: Shaopu
authors.initials: S
authors.lastname: Hu
authors.affiliation: Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, China.|Spine Institute, Shanghai University of Traditional Chinese Medicine, Shanghai, China.
authors.forename: Xuejun
authors.initials: X
authors.lastname: Cui
authors.affiliation: Division of Pulmonary and Critical Care Medicine, Department of Medicine, University of Alabama at Birmingham, Birmingham, AL, USA.
authors.forename: Gang
authors.initials: G
authors.lastname: Liu
authors.affiliation: Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, China.|Spine Institute, Shanghai University of Traditional Chinese Medicine, Shanghai, China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Shi
authors.affiliation: Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, China.|Spine Institute, Shanghai University of Traditional Chinese Medicine, Shanghai, China.|School of Rehabilitation Science, Shanghai University of Traditional Chinese Medicine, Shanghai, China.
authors.forename: Yongjun
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, China.|Spine Institute, Shanghai University of Traditional Chinese Medicine, Shanghai, China.
authors.forename: Yanping
authors.initials: Y
authors.lastname: Yang
mesh_terms: D000818:Animals; D014408:Biomarkers, Tumor / Q000235:genetics*; D001859:Bone Neoplasms / Q000235:genetics* / Q000473:pathology / Q000628:therapy; D016009:Chi-Square Distribution; D019295:Computational Biology; D030541:Databases, Genetic; D005260:Female; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D053263:Gene Regulatory Networks; D008297:Male; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008819:Mice, Nude; D016513:Mice, SCID; D035683:MicroRNAs / Q000235:genetics*; D016017:Odds Ratio; D012516:Osteosarcoma / Q000235:genetics* / Q000473:pathology / Q000628:therapy; D011237:Predictive Value of Tests; D011379:Prognosis; D059467:Transcriptome
chemical_list: D014408:Biomarkers, Tumor; D035683:MicroRNAs
keywords: meta-analysis; miRNA; mouse; osteosarcoma; therapeutic target