title: Atomic force microscopy characterization of kinase-mediated phosphorylation of a peptide monolayer.
abstract: We describe the detailed microscopic changes in a peptide monolayer following kinase-mediated phosphorylation. A reversible electrochemical transformation was observed using square wave voltammetry (SWV) in the reversible cycle of peptide phosphorylation by ERK2 followed by dephosphorylation by alkaline phosphatase. A newly developed method for analyzing local roughness, measured by atomic force microscope (AFM), showed a bimodal distribution. This may indicate either a hole-formation mechanism and/or regions on the surface in which the peptide changed its conformation upon phosphorylation, resulting in increased roughness and current. Our results provide the mechanistic basis for developing biosensors for detecting kinase-mediated phosphorylation in disease.
authors.affiliation: Institute of Chemistry and the Center for Nanoscience and Nanotechnology, The Hebrew University of Jerusalem, Safra Campus, Givat Ram, Jerusalem 91904, Israel.
authors.forename: Roman
authors.initials: R
authors.lastname: Zhuravel
authors.affiliation: Institute of Chemistry and the Center for Nanoscience and Nanotechnology, The Hebrew University of Jerusalem, Safra Campus, Givat Ram, Jerusalem 91904, Israel.
authors.forename: Einav
authors.initials: E
authors.lastname: Amit
authors.affiliation: Institute of Chemistry and the Center for Nanoscience and Nanotechnology, The Hebrew University of Jerusalem, Safra Campus, Givat Ram, Jerusalem 91904, Israel.
authors.forename: Shir
authors.initials: S
authors.lastname: Elbaz
authors.affiliation: Institute of Chemistry and the Center for Nanoscience and Nanotechnology, The Hebrew University of Jerusalem, Safra Campus, Givat Ram, Jerusalem 91904, Israel.
authors.forename: Dvir
authors.initials: D
authors.lastname: Rotem
authors.affiliation: Institute of Chemistry, Academia Sinica, Taipei, Taiwan.
authors.forename: Yu-Ju
authors.initials: YJ
authors.lastname: Chen
authors.affiliation: Institute of Chemistry and the Center for Nanoscience and Nanotechnology, The Hebrew University of Jerusalem, Safra Campus, Givat Ram, Jerusalem 91904, Israel.
authors.forename: Assaf
authors.initials: A
authors.lastname: Friedler
authors.affiliation: Institute of Chemistry and the Center for Nanoscience and Nanotechnology, The Hebrew University of Jerusalem, Safra Campus, Givat Ram, Jerusalem 91904, Israel.
authors.forename: Shlomo
authors.initials: S
authors.lastname: Yitzchaik
authors.affiliation: Institute of Chemistry and the Center for Nanoscience and Nanotechnology, The Hebrew University of Jerusalem, Safra Campus, Givat Ram, Jerusalem 91904, Israel.
authors.forename: Danny
authors.initials: D
authors.lastname: Porath
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D004563:Electrochemistry; D018625:Microscopy, Atomic Force / Q000379:methods*; D019950:Mitogen-Activated Protein Kinase 1 / Q000378:metabolism*; D010455:Peptides / Q000737:chemistry*; D010766:Phosphorylation
chemical_list: D010455:Peptides; D019950:Mitogen-Activated Protein Kinase 1; D000469:Alkaline Phosphatase