title: Self-Regulated Glucose-Sensitive Neoglycoenzyme-Capped Mesoporous Silica Nanoparticles for Insulin Delivery.
abstract: We describe herein the preparation of glucose-sensitive capped mesoporous silica nanoparticles for insulin delivery. The new material consists of an expanded-pore nanometric silica support grafted with 1-propyl-1-H-benzimidazole groups, loaded with fluorescein isothiocyanate-labeled insulin (FITC-Ins) and capped by the formation of inclusion complexes between cyclodextrin-modified glucose oxidase (CD-GOx) and the benzimidazole groups grafted on the mesoporous support. Insulin delivery from the gated material in simulated blood plasma was assessed upon addition of glucose. Glucose is transformed by GOx into gluconic acid, which promoted the dethreading of the benzimidazole-CD-GOx inclusion complexes, allowing cargo release. Small quantities of this support would be needed to release the amount of insulin necessary to decrease diabetic blood glucose concentrations to regular levels.
authors.affiliation: Instituto Interuniversitario de Investigación de Reconocimiento Molecular y Desarrollo Tecnológico, Unidad Mixta Universitat Politècnica de València, Universitat de València, Camino de Vera s/n, 46022, Valencia, Spain.|CIBER de Bioingeniería, Biomateriales y Nanomedicina (CIBER-BBN), Spain.
authors.forename: Mar
authors.initials: M
authors.lastname: Oroval
authors.affiliation: Nanosensors & Nanomachines Group, Department of Analytical Chemistry, Faculty of Chemistry, Complutense University of Madrid, 28040, Madrid, Spain.
authors.forename: Paula
authors.initials: P
authors.lastname: Díez
authors.affiliation: Instituto Interuniversitario de Investigación de Reconocimiento Molecular y Desarrollo Tecnológico, Unidad Mixta Universitat Politècnica de València, Universitat de València, Camino de Vera s/n, 46022, Valencia, Spain.|CIBER de Bioingeniería, Biomateriales y Nanomedicina (CIBER-BBN), Spain.
authors.forename: Elena
authors.initials: E
authors.lastname: Aznar
authors.affiliation: Instituto Interuniversitario de Investigación de Reconocimiento Molecular y Desarrollo Tecnológico, Unidad Mixta Universitat Politècnica de València, Universitat de València, Camino de Vera s/n, 46022, Valencia, Spain.|CIBER de Bioingeniería, Biomateriales y Nanomedicina (CIBER-BBN), Spain.
authors.forename: Carmen
authors.initials: C
authors.lastname: Coll
authors.affiliation: Instituto Interuniversitario de Investigación de Reconocimiento Molecular y Desarrollo Tecnológico, Unidad Mixta Universitat Politècnica de València, Universitat de València, Camino de Vera s/n, 46022, Valencia, Spain.|CIBER de Bioingeniería, Biomateriales y Nanomedicina (CIBER-BBN), Spain.|Departamento de Química, Universitat Politècnica de València, Camino de Vera s/n, 46022, València, Spain.
authors.forename: María Dolores
authors.initials: MD
authors.lastname: Marcos
authors.affiliation: Instituto Interuniversitario de Investigación de Reconocimiento Molecular y Desarrollo Tecnológico, Unidad Mixta Universitat Politècnica de València, Universitat de València, Camino de Vera s/n, 46022, Valencia, Spain.|CIBER de Bioingeniería, Biomateriales y Nanomedicina (CIBER-BBN), Spain.|Departamento de Química, Universitat Politècnica de València, Camino de Vera s/n, 46022, València, Spain.
authors.forename: Félix
authors.initials: F
authors.lastname: Sancenón
authors.affiliation: Nanosensors & Nanomachines Group, Department of Analytical Chemistry, Faculty of Chemistry, Complutense University of Madrid, 28040, Madrid, Spain.
authors.forename: Reynaldo
authors.initials: R
authors.lastname: Villalonga
authors.affiliation: Instituto Interuniversitario de Investigación de Reconocimiento Molecular y Desarrollo Tecnológico, Unidad Mixta Universitat Politècnica de València, Universitat de València, Camino de Vera s/n, 46022, Valencia, Spain.|CIBER de Bioingeniería, Biomateriales y Nanomedicina (CIBER-BBN), Spain.|Departamento de Química, Universitat Politècnica de València, Camino de Vera s/n, 46022, València, Spain.
authors.forename: Ramón
authors.initials: R
authors.lastname: Martínez-Máñez
mesh_terms: D001562:Benzimidazoles / Q000737:chemistry; D003505:Cyclodextrins / Q000737:chemistry; D004337:Drug Carriers / Q000737:chemistry*; D004800:Enzymes, Immobilized / Q000737:chemistry / Q000378:metabolism; D016650:Fluorescein-5-isothiocyanate / Q000031:analogs & derivatives* / Q000737:chemistry / Q000378:metabolism; D005942:Gluconates / Q000378:metabolism; D005947:Glucose / Q000737:chemistry / Q000378:metabolism; D005949:Glucose Oxidase / Q000737:chemistry; D007328:Insulin / Q000031:analogs & derivatives* / Q000737:chemistry / Q000378:metabolism; D053758:Nanoparticles / Q000737:chemistry*; D049329:Nanostructures / Q000737:chemistry; D016062:Porosity; D012822:Silicon Dioxide / Q000737:chemistry*; D013051:Spectrometry, Gamma
chemical_list: D001562:Benzimidazoles; D003505:Cyclodextrins; D004337:Drug Carriers; D004800:Enzymes, Immobilized; D005942:Gluconates; D007328:Insulin; C031573:insulin, fluorescein-isothiocyanated-; D012822:Silicon Dioxide; C031000:benzimidazole; D005949:Glucose Oxidase; D016650:Fluorescein-5-isothiocyanate; D005947:Glucose; C030691:gluconic acid
keywords: controlled release; glucose; insulin; mesoporous silica; molecular gates