title: The Mechanisms of Maize Resistance toFusarium verticillioidesby Comprehensive Analysis of RNA-seq Data.
abstract: Fusarium verticillioidesis the most commonly reported fungal species responsible for ear rot of maize which substantially reduces grain yield. It also results in a substantial accumulation of mycotoxins that give rise to toxic response when ingested by animals and humans. For inefficient control by chemical and agronomic measures, it thus becomes more desirable to select more resistant varieties. However, the molecular mechanisms underlying the infection process remain poorly understood, which hampers the application of quantitative resistance in breeding programs. Here, we reveal the disease-resistance mechanism of the maize inbred line of BT-1 which displays high resistance to ear rot using RNA high throughput sequencing. By analyzing RNA-seq data from the BT-1 kernels before and afterF. verticillioidesinoculation, we found that transcript levels of genes associated with key pathways are dramatically changed compared with the control treatment. Differential gene expression in ear rot resistant and susceptible maize was confirmed by RNA microarray and qRT-PCR analyses. Further investigation suggests that the small heat shock protein family, some secondary metabolites, and the signaling pathways of abscisic acid, jasmonic acid, or salicylic acids (SA) may be involved in the pathogen-associated molecular pattern-triggered immunity againstF. verticillioides. These data will not only provide new insights into the molecular resistant mechanisms against fungi invading, but may also result in the identification of key molecular factors associated with ear rot resistance in maize.
authors.affiliation: College of Agronomy, Synergetic Innovation Center of Henan Grain Crops and National Key Laboratory of Wheat and Maize Crop Science, Henan Agricultural University Zhengzhou, China.
authors.forename: Yanping
authors.initials: Y
authors.lastname: Wang
authors.affiliation: College of Agronomy, Synergetic Innovation Center of Henan Grain Crops and National Key Laboratory of Wheat and Maize Crop Science, Henan Agricultural University Zhengzhou, China.
authors.forename: Zijian
authors.initials: Z
authors.lastname: Zhou
authors.affiliation: College of Agronomy, Synergetic Innovation Center of Henan Grain Crops and National Key Laboratory of Wheat and Maize Crop Science, Henan Agricultural University Zhengzhou, China.
authors.forename: Jingyang
authors.initials: J
authors.lastname: Gao
authors.affiliation: College of Agronomy, Synergetic Innovation Center of Henan Grain Crops and National Key Laboratory of Wheat and Maize Crop Science, Henan Agricultural University Zhengzhou, China.
authors.forename: Yabin
authors.initials: Y
authors.lastname: Wu
authors.affiliation: College of Agronomy, Synergetic Innovation Center of Henan Grain Crops and National Key Laboratory of Wheat and Maize Crop Science, Henan Agricultural University Zhengzhou, China.
authors.forename: Zongliang
authors.initials: Z
authors.lastname: Xia
authors.affiliation: College of Agronomy, Synergetic Innovation Center of Henan Grain Crops and National Key Laboratory of Wheat and Maize Crop Science, Henan Agricultural University Zhengzhou, China.
authors.forename: Huiyong
authors.initials: H
authors.lastname: Zhang
authors.affiliation: College of Agronomy, Synergetic Innovation Center of Henan Grain Crops and National Key Laboratory of Wheat and Maize Crop Science, Henan Agricultural University Zhengzhou, China.
authors.forename: Jianyu
authors.initials: J
authors.lastname: Wu
keywords: Fusarium verticillioides; PTI; RNA-seq; ear rot; hormone signaling; maize; sHSPs; secondary metabolites