title: Vitamin B12deficiency results in severe oxidative stress, leading to memory retention impairment in Caenorhabditis elegans.
abstract: Oxidative stress is implicated in various human diseases and conditions, such as a neurodegeneration, which is the major symptom of vitamin B12deficiency, although the underlying disease mechanisms associated with vitamin B12deficiency are poorly understood. Vitamin B12deficiency was found to significantly increase cellular H2O2and NO content in Caenorhabditis elegans and significantly decrease low molecular antioxidant [reduced glutathione (GSH) and L-ascorbic acid] levels and antioxidant enzyme (superoxide dismutase and catalase) activities, indicating that vitamin B12deficiency induces severe oxidative stress leading to oxidative damage of various cellular components in worms. An NaCl chemotaxis associative learning assay indicated that vitamin B12deficiency did not affect learning ability but impaired memory retention ability, which decreased to approximately 58% of the control value. When worms were treated with 1mmol/L GSH, L-ascorbic acid, or vitamin E for three generations during vitamin B12deficiency, cellular malondialdehyde content as an index of oxidative stress decreased to the control level, but the impairment of memory retention ability was not completely reversed (up to approximately 50%). These results suggest that memory retention impairment formed during vitamin B12deficiency is partially attributable to oxidative stress.
authors.affiliation: The School of Agricultural, Biological and Environmental sciences, Faculty of Agriculture, Tottori University, Tottori 680-8533, Japan.
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Bito
authors.affiliation: The School of Agricultural, Biological and Environmental sciences, Faculty of Agriculture, Tottori University, Tottori 680-8533, Japan.
authors.forename: Taihei
authors.initials: T
authors.lastname: Misaki
authors.affiliation: The School of Agricultural, Biological and Environmental sciences, Faculty of Agriculture, Tottori University, Tottori 680-8533, Japan.
authors.forename: Yukinori
authors.initials: Y
authors.lastname: Yabuta
authors.affiliation: Department of Life Science and Biotechnology, Faculty of Life and Environmental Science, Shimane University, Shimane 690-8504, Japan.
authors.forename: Takahiro
authors.initials: T
authors.lastname: Ishikawa
authors.affiliation: The School of Agricultural, Biological and Environmental sciences, Faculty of Agriculture, Tottori University, Tottori 680-8533, Japan.
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Kawano
authors.affiliation: The School of Agricultural, Biological and Environmental sciences, Faculty of Agriculture, Tottori University, Tottori 680-8533, Japan. Electronic address: watanabe@muses.tottori-u.ac.jp.
authors.forename: Fumio
authors.initials: F
authors.lastname: Watanabe
mesh_terms: D000818:Animals; D001205:Ascorbic Acid / Q000378:metabolism; D017173:Caenorhabditis elegans / Q000235:genetics* / Q000378:metabolism / Q000502:physiology; D002374:Catalase / Q000235:genetics / Q000378:metabolism; D002633:Chemotaxis / Q000187:drug effects / Q000235:genetics; D005978:Glutathione / Q000378:metabolism; D006801:Humans; D006861:Hydrogen Peroxide / Q000378:metabolism; D007858:Learning / Q000187:drug effects / Q000502:physiology*; D008568:Memory / Q000187:drug effects / Q000502:physiology*; D009410:Nerve Degeneration / Q000235:genetics* / Q000503:physiopathology; D009569:Nitric Oxide / Q000378:metabolism; D018384:Oxidative Stress / Q000235:genetics / Q000502:physiology; D012965:Sodium Chloride / Q000633:toxicity; D013482:Superoxide Dismutase / Q000235:genetics / Q000378:metabolism; D014805:Vitamin B 12 / Q000235:genetics / Q000378:metabolism; D014806:Vitamin B 12 Deficiency / Q000235:genetics* / Q000503:physiopathology
chemical_list: D009569:Nitric Oxide; D012965:Sodium Chloride; D006861:Hydrogen Peroxide; D002374:Catalase; D013482:Superoxide Dismutase; D005978:Glutathione; D014805:Vitamin B 12; D001205:Ascorbic Acid
keywords: Caenorhabditis elegans; Cobalamin; Homocysteine; Methionine synthase; Oxidative stress; Vitamin B(12)