title: [Influences of DNA methylation upon neuroglobin sustained expression in oxygen- glucose deprivation model].
abstract: Objective:To investigate the influences of genomic DNA methylation upon neuroglobin sustained expression in oxygen- glucose deprivation model.Methods:With A549 cell strain as the research object, the control group were cultivated in the complete medium containing 10 μmol/L of 5-azacytidine for 4 days, and the control group was cultivated in the complete medium for 4 days.Then carried out oxygen glucose deprivation treatment for 4 h.Detecting neuroglobin expression, DNA methyltransferase expression, cell inhibition ratio and DNA methylation level at different time points.Results:DNA methylation level of the experimental group declined apparently[6 h : (1.0±0.0) vs (2.1±0.3); 12 h: ( 0.9±0.0) vs (1.4±0.0); 24 h: (0.9±0.0) vs (2.6±0.2); 36 h: (0.9±0.0) vs (2.9±0.1)], neuroglobin expression of the experimental group continued and was obviously higher than that of the control group at the same time point[NGB-PCR: 6 h: (3.3±1.1) vs (0.4±0.1); 12 h: (3.2±0.8) vs (0.1±0.1); 24 h: (4.6±0.6) vs (0.2±0.0); 36 h : (5.1±0.3) vs (0.1±0.1)], while the Cell inhibition ratio of the experimental group was obviously lower than that of the control group at the same time point[(6 h: (10.4±0.5) vs (14.1±0.7); 12 h: (22.0±1.3) vs (35.1±0.5); 24 h: (25.7±1.0) vs (40.6±1.3); 36 h: (30.0±0.8) vs (44.4±0.7)], differences had statistical significance (P<0.05).mRNA expression of three methyltransferases of the experimental group was higher than that of the control group at different time points, where, DNMT1 and DNMT3B showed great differences (P<0.05), while differences in DNMT3A of two groups had no statistical significance (P>0.05).Conclusions:In the OGD/R model of A549 cell strain, genomic DNA methylation resulted in unsustained expression of neuroglobin, but neuroglobin expression increased after demethylation inhibitor was used.
authors.affiliation: Department of Critical Care Medicine, Xiangya Hospital, Central South University, Changsha 410008, China.
authors.forename: L N
authors.initials: LN
authors.lastname: Zhang
authors.forename: S B
authors.initials: SB
authors.lastname: Hu
authors.forename: S Y
authors.initials: SY
authors.lastname: Deng
authors.forename: C X
authors.initials: CX
authors.lastname: Chen
authors.forename: L
authors.initials: L
authors.lastname: Wu
authors.forename: Q Y
authors.initials: QY
authors.lastname: Peng
authors.forename: L
authors.initials: L
authors.lastname: Huang
authors.forename: Y H
authors.initials: YH
authors.lastname: Ai
mesh_terms: D001374:Azacitidine; D004248:DNA (Cytosine-5-)-Methyltransferases; D019175:DNA Methylation; D005914:Globins; D005947:Glucose; D006801:Humans; D009419:Nerve Tissue Proteins; D000078787:Neuroglobin; D010100:Oxygen; D000097584:DNA Methyltransferase 3B
chemical_list: D001374:Azacitidine; D004248:DNA (Cytosine-5-)-Methyltransferases; D005914:Globins; D005947:Glucose; D009419:Nerve Tissue Proteins; D000078787:Neuroglobin; D010100:Oxygen; D000097584:DNA Methyltransferase 3B