title: Proliferative Effects of Histamine on Primary Human Pterygium Fibroblasts.
abstract: Purpose. It has been confirmed that inflammatory cytokines are involved in the progression of pterygium. Histamine can enhance proliferation and migration of many cells. Therefore, we intend to investigate the proliferative and migratory effects of histamine on primary culture of human pterygium fibroblasts (HPFs).Methods. Pterygium and conjunctiva samples were obtained from surgery, and toluidine blue staining was used to identify mast cells. 3-[4, 5-Dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) was performed to evaluate the proliferative rate of HPFs and human conjunctival fibroblasts (HCFs); ki67 expression was also measured by immunofluorescence analysis. Histamine receptor-1 (H1R) antagonist (Diphenhydramine Hydrochloride) and histamine receptor-2 (H2R) antagonist (Nizatidine) were added to figure out which receptor was involved. Wound healing model was used to evaluate the migratory ability of HPFs.Results. The numbers of total mast cells and degranulated mast cells were both higher in pterygium than in conjunctiva. Histamine had a proliferative effect on both HPFs and HCFs, the effective concentration (10μmol/L) on HPFs was lower than on HCFs (100μmol/L), and the effect could be blocked by H1R antagonist. Histamine showed no migratory effect on HPFs.Conclusion. Histamine may play an important role in the proliferation of HPFs and act through H1R.
authors.affiliation: Eye Center of the 2nd Affiliated Hospital, Medical College of Zhejiang University, Hangzhou, Zhejiang, China; Zhejiang Provincial Key Lab of Ophthalmology, Hangzhou, Zhejiang, China.
authors.forename: Zhenwei
authors.identifier: 0000-0002-0040-1416
authors.initials: Z
authors.lastname: Qin
authors.affiliation: Eye Center of the 2nd Affiliated Hospital, Medical College of Zhejiang University, Hangzhou, Zhejiang, China; Zhejiang Provincial Key Lab of Ophthalmology, Hangzhou, Zhejiang, China.
authors.forename: Qiuli
authors.initials: Q
authors.lastname: Fu
authors.affiliation: Eye Center of the 2nd Affiliated Hospital, Medical College of Zhejiang University, Hangzhou, Zhejiang, China; Zhejiang Provincial Key Lab of Ophthalmology, Hangzhou, Zhejiang, China.
authors.forename: Lifang
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Eye Center of the 2nd Affiliated Hospital, Medical College of Zhejiang University, Hangzhou, Zhejiang, China; Zhejiang Provincial Key Lab of Ophthalmology, Hangzhou, Zhejiang, China.
authors.forename: Houfa
authors.initials: H
authors.lastname: Yin
authors.affiliation: Eye Center of the 2nd Affiliated Hospital, Medical College of Zhejiang University, Hangzhou, Zhejiang, China; Zhejiang Provincial Key Lab of Ophthalmology, Hangzhou, Zhejiang, China.
authors.forename: Xiuming
authors.initials: X
authors.lastname: Jin
authors.affiliation: Eye Center of the 2nd Affiliated Hospital, Medical College of Zhejiang University, Hangzhou, Zhejiang, China; Zhejiang Provincial Key Lab of Ophthalmology, Hangzhou, Zhejiang, China.
authors.forename: Qiaomei
authors.initials: Q
authors.lastname: Tang
authors.affiliation: Eye Center of the 2nd Affiliated Hospital, Medical College of Zhejiang University, Hangzhou, Zhejiang, China; Zhejiang Provincial Key Lab of Ophthalmology, Hangzhou, Zhejiang, China.
authors.forename: Danni
authors.initials: D
authors.lastname: Lyu
authors.affiliation: Eye Center of the 2nd Affiliated Hospital, Medical College of Zhejiang University, Hangzhou, Zhejiang, China; Zhejiang Provincial Key Lab of Ophthalmology, Hangzhou, Zhejiang, China.
authors.forename: Ke
authors.identifier: 0000-0002-6764-7365
authors.initials: K
authors.lastname: Yao
mesh_terms: D049109:Cell Proliferation / Q000187:drug effects / Q000502:physiology; D002478:Cells, Cultured; D003228:Conjunctiva / Q000166:cytology; D004155:Diphenhydramine / Q000494:pharmacology; D005347:Fibroblasts / Q000187:drug effects* / Q000378:metabolism*; D006632:Histamine / Q000494:pharmacology*; D006634:Histamine H1 Antagonists / Q000494:pharmacology; D006801:Humans; D008407:Mast Cells / Q000187:drug effects / Q000378:metabolism; D016567:Nizatidine / Q000494:pharmacology; D011625:Pterygium / Q000473:pathology*; D060888:Real-Time Polymerase Chain Reaction; D044967:Serum / Q000502:physiology
chemical_list: D006634:Histamine H1 Antagonists; D006632:Histamine; D004155:Diphenhydramine; D016567:Nizatidine