title: Mathematical modeling of the whole expanded bed adsorption process to recover and purify chitosanases from the unclarified fermentation broth of Paenibacillus ehimensis.
abstract: In this study, a general rate model was applied to the entire process of expanded bed adsorption chromatography (EBAC) for the chitosanases purification protocol from unclarified fermentation broth produced by Paenibacillus ehimensis using the anionic adsorbent Streamline®DEAE. For the experiments performed using the expanded bed, a homemade column (2.6cm×30.0cm) was specially designed. The proposed model predicted the entire EBA process adequately, giving R2values higher than 0.85 and χ2as low as 0.351 for the elution step. Using the validated model, a 33factorial design was used to investigate other non-tested conditions as input. It was observed that the superficial velocity during loading and washing steps, as well as the settled bed height, has a strong positive effect on the F objective function used to evaluate the production of the purified chitosanases.
authors.affiliation: Biochemical Engineering Laboratory, Chemical Engineering Department, Federal University of Rio Grande do Norte (UFRN), Natal, RN, Brazil. Electronic address: everaldo.eq@gmail.com.
authors.forename: Carlos Eduardo
authors.initials: CE
authors.lastname: de Araújo Padilha
authors.affiliation: Biochemical Engineering Laboratory, Chemical Engineering Department, Federal University of Rio Grande do Norte (UFRN), Natal, RN, Brazil.
authors.forename: Paulo Victor
authors.initials: PV
authors.lastname: Fortunato Dantas
authors.affiliation: Biochemical Engineering Laboratory, Chemical Engineering Department, Federal University of Rio Grande do Norte (UFRN), Natal, RN, Brazil.
authors.forename: Francisco Canindé
authors.initials: FC
authors.lastname: de Sousa
authors.affiliation: Biochemical Engineering Laboratory, Chemical Engineering Department, Federal University of Rio Grande do Norte (UFRN), Natal, RN, Brazil.
authors.forename: Domingos Fabiano
authors.initials: DF
authors.lastname: de Santana Souza
authors.affiliation: Biochemical Engineering Laboratory, Chemical Engineering Department, Federal University of Rio Grande do Norte (UFRN), Natal, RN, Brazil.
authors.forename: Jackson Araújo
authors.initials: JA
authors.lastname: de Oliveira
authors.affiliation: Biochemical Engineering Laboratory, Chemical Engineering Department, Federal University of Rio Grande do Norte (UFRN), Natal, RN, Brazil.
authors.forename: Gorete Ribeiro
authors.initials: GR
authors.lastname: de Macedo
authors.affiliation: Biochemical Engineering Laboratory, Chemical Engineering Department, Federal University of Rio Grande do Norte (UFRN), Natal, RN, Brazil.
authors.forename: Everaldo Silvino
authors.initials: ES
authors.lastname: Dos Santos
mesh_terms: D000327:Adsorption; D002852:Chromatography, Ion Exchange / Q000379:methods*; D005285:Fermentation; D006026:Glycoside Hydrolases / Q000032:analysis / Q000302:isolation & purification*; D008956:Models, Chemical; D056507:Paenibacillus / Q000201:enzymology*
chemical_list: D006026:Glycoside Hydrolases; C019868:chitosanase
keywords: Chitosanases; Expanded bed adsorption; Experimental design; Fermentation broth; Mathematical modeling