title: Osteogenic potential of stem cells-seeded bioactive nanocomposite scaffolds: A comparative study between human mesenchymal stem cells derived from bone, umbilical cord Wharton's jelly, and adipose tissue.
abstract: Bone regeneration is considered as an unmet clinical need, the aim of this study is to investigate the osteogenic potential of three different mesenchymal stem cells (MSCs) derived from human bone marrow (BM-MSCs), umbilical cord Wharton's jelly (UC-MSCs), and adipose (AD-MSCs) seeded on a recently developed nanocomposite scaffold (bioactive glass/gelatin) implanted in rat animal models with critical size calvarial defects. In this study, after isolation, culture, and characterization, the MSCs were expanded and seeded on the scaffolds for in vitro and in vivo studies. The adhesion, proliferation, and viability of the cells on the scaffolds evaluated in vitro, showed that the scaffolds were biocompatible for further examinations. In order to evaluate the scaffolds in vivo, rat animal models with critical size calvarial defects were randomly categorized in four groups and treated with the scaffolds. The animals were sacrificed at the time points of 4 and 12 weeks of post-implantation, bone healing process were investigated. The histological and immunohistological observations showed (p < 0.01) higher osteogenesis capacity in the group treated with BM-MSCs/scaffolds compared to the other groups. However, the formation of new angiogenesis was evidently higher in the defects filled with UC-MSCs/scaffolds. This preliminary study provides promising data for further clinical trials. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 106B: 61-72, 2018.
authors.affiliation: Department of Tissue Engineering and Applied Cell Sciences, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran.|National Cell Bank, Pasteur Institute of Iran, Tehran, Iran.
authors.forename: Saeid
authors.initials: S
authors.lastname: Kargozar
authors.affiliation: Department of Tissue Engineering and Regenerative Medicine, Faculty of Advanced Technologies in Medicine, Iran University of Medical Sciences, Tehran, Iran.|Cellular and Molecular Research Center (CMRC), Iran University of Medical Sciences, Tehran, Iran.
authors.forename: Masoud
authors.identifier: http://orcid.org/0000-0002-0232-352X
authors.initials: M
authors.lastname: Mozafari
authors.affiliation: Diabetes Research Center, Endocrinology and Metabolism Clinical Sciences Institute, Tehran University of Medical Sciences, Tehran, Iran.|Endocrinology and Metabolism Research Center, Endocrinology and Metabolism Research Institute, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Seyed Jafar
authors.initials: SJ
authors.lastname: Hashemian
authors.affiliation: Department of Tissue Engineering and Regenerative Medicine, Faculty of Advanced Technologies in Medicine, Iran University of Medical Sciences, Tehran, Iran.|Cellular and Molecular Research Center (CMRC), Iran University of Medical Sciences, Tehran, Iran.
authors.forename: Peiman
authors.initials: P
authors.lastname: Brouki Milan
authors.affiliation: Department of Medical Genetics, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Sepideh
authors.initials: S
authors.lastname: Hamzehlou
authors.affiliation: Cellular and Molecular Research Center (CMRC), Iran University of Medical Sciences, Tehran, Iran.|Department of Anatomy, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran.
authors.forename: Mansooreh
authors.initials: M
authors.lastname: Soleimani
authors.affiliation: Cellular and Molecular Research Center (CMRC), Iran University of Medical Sciences, Tehran, Iran.|Department of Anatomy, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran.
authors.forename: Mohammad Taghi
authors.initials: MT
authors.lastname: Joghataei
authors.affiliation: Department of Tissue Engineering and Regenerative Medicine, Faculty of Advanced Technologies in Medicine, Iran University of Medical Sciences, Tehran, Iran.|Cellular and Molecular Biology Research Centre, Shahid Beheshti University of Medical Science, Tehran, Iran.
authors.forename: Mazaher
authors.initials: M
authors.lastname: Gholipourmalekabadi
authors.affiliation: Cellular and Molecular Research Center (CMRC), Iran University of Medical Sciences, Tehran, Iran.
authors.forename: Alireza
authors.initials: A
authors.lastname: Korourian
authors.affiliation: Cellular and Molecular Research Center (CMRC), Iran University of Medical Sciences, Tehran, Iran.|Department of Molecular Medicine, Faculty of Advanced Technologies in Medicine, Iran University of Medical Sciences, Tehran, Iran.
authors.forename: Kazem
authors.initials: K
authors.lastname: Mousavizadeh
authors.affiliation: Division of Surgery and Interventional Science, UCL Centre for Nanotechnology and Regenerative Medicine, University College London, London, UK.
authors.forename: Alexander M
authors.identifier: http://orcid.org/0000-0002-8334-9376
authors.initials: AM
authors.lastname: Seifalian
mesh_terms: D000273:Adipose Tissue / Q000166:cytology / Q000378:metabolism*; D001854:Bone Marrow Cells; D002469:Cell Separation; D005260:Female; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism*; D053761:Nanocomposites / Q000737:chemistry*; D009928:Organ Specificity; D010012:Osteogenesis; D054457:Tissue Scaffolds / Q000737:chemistry*
keywords: Bioactive glass; Bone regeneration; Mesenchymal stem cells; Scaffold