title: Nanoscale hydration dynamics of DNA-lipid blend dry films: DNA-size dependency.
abstract: In this study, nanoscale hydration dynamics of DNA-lipid blend dry films are investigated via small angle X-ray diffraction. Compared to the hydration of lipid films, fragmented short DNA strands and counterions in stacked lipid layers dramatically accelerate both the relaxation of the lamellar distance to a metastable interval and the subsequent peeling-off process of lipid bilayers. Moreover, genome-sized long DNA and counterions accelerate the relaxation process, but suppress the peeling-off process and simultaneously induce a damped-oscillation of the lamellar interval; this is probably due to the viscoelastic properties of the entangled long DNA dissolved in hydrated water between the stacked lipid bilayers. This study's findings can pave the way for producing cell-sized liposomes, which efficiently encapsulate any arbitrary sized DNA through natural swelling.
authors.affiliation: Department of Chemistry, Ecole Normale Supérieure-PSL Research University, 75005 Paris, France. shimobayashi@jamstec.go.jp.
authors.forename: S F
authors.initials: SF
authors.lastname: Shimobayashi
authors.affiliation: Department of Chemistry, Faculty of Pure and Applied Sciences, University of Tsukuba, Ibaraki 305-8571, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Hishida
authors.affiliation: Department of Physics, Graduate School of Science, Kyoto University, Kyoto 606-8502, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Kurimura
authors.affiliation: Department of Physics, Graduate School of Science, Kyoto University, Kyoto 606-8502, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Ichikawa
mesh_terms: D004247:DNA / Q000737:chemistry*; D008051:Lipid Bilayers / Q000737:chemistry*; D010316:Particle Size; D010713:Phosphatidylcholines / Q000737:chemistry; D053838:Scattering, Small Angle; D014867:Water / Q000737:chemistry*; D014961:X-Ray Diffraction
chemical_list: D008051:Lipid Bilayers; D010713:Phosphatidylcholines; D014867:Water; D004247:DNA; C017251:1,2-oleoylphosphatidylcholine