title: Evaluation of functionalized mesoporous silica SBA-15 as a carrier system for Ph3Sn(CH2)3OH against the A2780 ovarian carcinoma cell line.
abstract: SBA-15|Sn3, a mesoporous silica-based material (derivative of SBA-15) loaded with an organotin compound Ph3Sn(CH2)3OH (Sn3), possesses improved antitumor potential against the A2780 high-grade serous ovarian carcinoma cell line in comparison to Sn3. It is demonstrated that both the compound and the nanostructured material are internalized by the A2780 cells. A similar mode of action of Sn3 and SBA-15|Sn3 against the A2780 cell line was found. Explicitly, induction of apoptosis, caspase 2, 3, 8 and 9 activation, accumulation of cells in the hypodiploid phase as well as accumulation of ROS were observed. Interestingly, Sn3 loaded in the mesoporous silica-based material needed to reach a concentration 3.5 times lower than the IC50value of the Sn3 compound, pointing out a higher effect of the SBA-15|Sn3 than Sn3 alone. Clonogenic potential, growth in 3D culture as well as mobility of cells were disturbed in the presence of SBA-15|Sn3. Such behavior could be associated with the suppression of p-38 MAPK. Less profound effect of Sn3 compared to SBA-15|Sn3 could be attributed to a different regulation of p-38 and STAT-3, which are mainly responsible for an appropriate cellular response to diverse stimuli or metastatic properties.
authors.affiliation: Institute of Chemistry, Martin Luther University Halle-Wittenberg, Kurt-Mothes-Straße 2, D-06120 Halle, Germany.
authors.forename: Christian
authors.initials: C
authors.lastname: Bensing
authors.forename: Marija
authors.initials: M
authors.lastname: Mojić
authors.forename: Santiago
authors.initials: S
authors.lastname: Gómez-Ruiz
authors.forename: Sandra
authors.initials: S
authors.lastname: Carralero
authors.forename: Biljana
authors.initials: B
authors.lastname: Dojčinović
authors.forename: Danijela
authors.initials: D
authors.lastname: Maksimović-Ivanić
authors.forename: Sanja
authors.initials: S
authors.lastname: Mijatović
authors.forename: Goran N
authors.initials: GN
authors.lastname: Kaluđerović
mesh_terms: D000970:Antineoplastic Agents / Q000008:administration & dosage* / Q000737:chemistry / Q000494:pharmacology; D020169:Caspases / Q000378:metabolism; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D004337:Drug Carriers / Q000008:administration & dosage* / Q000737:chemistry / Q000494:pharmacology; D005260:Female; D006801:Humans; D009947:Organotin Compounds / Q000008:administration & dosage* / Q000737:chemistry / Q000494:pharmacology; D010051:Ovarian Neoplasms / Q000188:drug therapy / Q000378:metabolism / Q000473:pathology; D016062:Porosity; D017382:Reactive Oxygen Species / Q000378:metabolism; D050796:STAT3 Transcription Factor / Q000378:metabolism; D012822:Silicon Dioxide / Q000008:administration & dosage* / Q000737:chemistry / Q000494:pharmacology; D048051:p38 Mitogen-Activated Protein Kinases / Q000378:metabolism
chemical_list: D000970:Antineoplastic Agents; D004337:Drug Carriers; D009947:Organotin Compounds; D017382:Reactive Oxygen Species; C509969:SBA-15; D050796:STAT3 Transcription Factor; C494086:STAT3 protein, human; D012822:Silicon Dioxide; D048051:p38 Mitogen-Activated Protein Kinases; D020169:Caspases