title: Arabidopsis phospholipase D alpha 1-derived phosphatidic acid regulates microtubule organization and cell development under microtubule-interacting drugs treatment.
abstract: Phospholipase D (PLD) and its product phosphatidic acid (PA) are emerging as essential regulators of cytoskeleton organization in plants. However, the underlying molecular mechanisms of PA-mediated microtubule reorganization in plants remain largely unknown. In this study, we used pharmacological and genetic approaches to analyze the function of Arabidopsis thaliana PLDα1 in the regulation of microtubule organization and cell development in response to microtubule-affecting drugs. Treatment with the microtubule-stabilizing drug paclitaxel resulted in less growth inhibition and decreased rightward slant of roots, longitudinal alignment of microtubules, and enhanced length of hypocotyl epidermal cells in the pldα1 mutant, the phenotype of which was rescued by exogenous application of PA. Moreover, the pldα1 mutant was sensitive to the microtubule-disrupting drugs oryzalin and propyzamide in terms of seedling survival ratio, left-skewing angle of roots and microtubule organization. In addition, both disruption and stabilization of microtubules induced by drugs activated PLDα1 activity. Our findings demonstrate that in A. thaliana, PLDα1/PA might regulate cell development by modulating microtubule organization in an activity-dependent manner.
authors.affiliation: College of Life Sciences, State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing, 210095, People's Republic of China. zhangqun@njau.edu.cn.
authors.forename: Qun
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: College of Life Sciences, State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing, 210095, People's Republic of China.
authors.forename: Yana
authors.initials: Y
authors.lastname: Qu
authors.affiliation: College of Life Sciences, State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing, 210095, People's Republic of China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Wang
authors.affiliation: College of Life Sciences, State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing, 210095, People's Republic of China.
authors.forename: Ping
authors.initials: P
authors.lastname: Song
authors.affiliation: College of Life Sciences, State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing, 210095, People's Republic of China.
authors.forename: Peipei
authors.initials: P
authors.lastname: Wang
authors.affiliation: College of Life Sciences, State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing, 210095, People's Republic of China.
authors.forename: Qianru
authors.initials: Q
authors.lastname: Jia
authors.affiliation: College of Life Sciences, State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing, 210095, People's Republic of China.
authors.forename: Jinhe
authors.initials: J
authors.lastname: Guo
mesh_terms: D017360:Arabidopsis / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D004136:Dinitrobenzenes / Q000494:pharmacology*; D055786:Gene Knockout Techniques; D008870:Microtubules / Q000187:drug effects* / Q000648:ultrastructure; D009154:Mutation; D017239:Paclitaxel / Q000494:pharmacology*; D010712:Phosphatidic Acids / Q000378:metabolism*; D010739:Phospholipase D / Q000235:genetics / Q000378:metabolism*; D018517:Plant Roots / Q000187:drug effects / Q000201:enzymology / Q000235:genetics / Q000254:growth & development; D036226:Seedlings / Q000187:drug effects / Q000201:enzymology / Q000235:genetics / Q000254:growth & development; D013424:Sulfanilamides / Q000494:pharmacology*
chemical_list: D029681:Arabidopsis Proteins; D004136:Dinitrobenzenes; D010712:Phosphatidic Acids; D013424:Sulfanilamides; C012465:oryzalin; C582894:PLDA1 protein, Arabidopsis; D010739:Phospholipase D; D017239:Paclitaxel
keywords: Cell development; Microtubule; Paclitaxel; Phosphatidic acid; Phospholipase D