title: Role of DNA methylation in bisphenol A exposed mouse spermatocyte.
abstract: As a widespread environmental contaminant, bisphenol A (2,2-bis(4-hydroxyphenyl)propane, BPA) has been implicated in male reproductive function injury. Previous studies have investigated the mechanisms of DNA damage and oxidative stress caused by BPA; however, little is known regarding its impact on DNA methylation. In this paper, we assessed the adverse effects of BPA on mouse spermatocytes and investigated a potential role of DNA methylation. We demonstrated that BPA exposure inhibited cell proliferation, reduced the DNA replication capacity, and triggered apoptosis in GC-2 cells. In addition, the global DNA methylation levels increased, and the relative expression levels of DNA methyltransferases (DNMTs) varied following BPA exposure. Thousands of distinct methylated sites were screened using microarray analysis. The expressions of myosin-binding protein H (mybph) and protein kinase C δ (prkcd) were verified to be regulated by DNA methylation. These findings indicate that BPA had toxicity in spermatocytes, and DNA methylation may play a vital role in the regulation of BPA-triggered spermatocyte toxicity.
authors.affiliation: Institute of Toxicology, College of Preventive Medicine, Third Military Medical University, Chongqing, 4000382, China.
authors.forename: Li
authors.initials: L
authors.lastname: Yin
authors.affiliation: Institute of Toxicology, College of Preventive Medicine, Third Military Medical University, Chongqing, 4000382, China; Medical Laboratory Technology Department, Chuxiong Medical College, Yunnan, 675005, China.
authors.forename: Yanlin
authors.initials: Y
authors.lastname: Dai
authors.affiliation: Institute of Toxicology, College of Preventive Medicine, Third Military Medical University, Chongqing, 4000382, China.
authors.forename: Xiao
authors.initials: X
authors.lastname: Jiang
authors.affiliation: Institute of Toxicology, College of Preventive Medicine, Third Military Medical University, Chongqing, 4000382, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Institute of Toxicology, College of Preventive Medicine, Third Military Medical University, Chongqing, 4000382, China.
authors.forename: Hongqiang
authors.initials: H
authors.lastname: Chen
authors.affiliation: Institute of Toxicology, College of Preventive Medicine, Third Military Medical University, Chongqing, 4000382, China.
authors.forename: Fei
authors.initials: F
authors.lastname: Han
authors.affiliation: Institute of Toxicology, College of Preventive Medicine, Third Military Medical University, Chongqing, 4000382, China.
authors.forename: Jia
authors.initials: J
authors.lastname: Cao
authors.affiliation: Institute of Toxicology, College of Preventive Medicine, Third Military Medical University, Chongqing, 4000382, China. Electronic address: jinyiliutmmu@163.com.
authors.forename: Jinyi
authors.initials: J
authors.lastname: Liu
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D001559:Benzhydryl Compounds / Q000633:toxicity*; D018929:Cell Culture Techniques; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D018899:CpG Islands / Q000187:drug effects; D004248:DNA (Cytosine-5-)-Methyltransferases / Q000235:genetics; D019175:DNA Methylation / Q000187:drug effects* / Q000235:genetics; D004261:DNA Replication / Q000187:drug effects; D004785:Environmental Pollutants / Q000633:toxicity*; D008297:Male; D051379:Mice; D010636:Phenols / Q000633:toxicity*; D013090:Spermatocytes / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D000098826:Bisphenol A Compounds
chemical_list: D001559:Benzhydryl Compounds; D004248:DNA (Cytosine-5-)-Methyltransferases; D004785:Environmental Pollutants; D010636:Phenols; C006780:bisphenol A; D000098826:Bisphenol A Compounds
keywords: Bisphenol A; DNA methylation; DNA methyltransferase; Spermatocyte toxicity