title: The investigation of anti-inflammatory activity of Yi Guanjian decoction by serum metabonomics approach.
abstract: Yi Guanjian (YGJ), one of the Chinese herbal medicines most commonly used in western countries, reported to possess significant anti-inflammatary effects that inhibit the process of inflammation. However, the mechanisms underlying its anti-inflammation effects remain largely unresolved. This study was aimed to investigate the anti-inflammatory activity of YGJ and to explore its potential anti-inflammatory mechanisms by serum metabonomics approach. An xylene-induced mouse right-ear-edema model was used as an inflammatory response in vivo model. Ear edema, prostaglandin E2 (PGE2) and Tumor-Necrosis-Factor-alpha (TNF-α) were detected. Then, serum metabolic profiling was analyzed and pathway analysis performed on the biomarkers reversed after YGJ administration and further integration of metabolic networks. The results showed that YGJ alleviated ear edema and decreased serum PGE2and TNF-α levels. Fourteen biomarkers were screened, and the levels were all reversed to different degrees after YGJ administration. These biomarkers were mainly related to linoleic acid metabolism, taurine and hypotaurine metabolism, glyoxylate and dicarboxylate metabolism, glycine, serine and threonine metabolism and citrate cycle (TCA cycle). In metabolic networks, glycine and pyruvate were node molecules. This indicated that YGJ could significantly inhibit inflammatory response triggered by acute local stimulation and exerted anti-inflammatory activity mainly by regulating node molecules.
authors.affiliation: Anhui Medical University, Hefei 230032, China; Institute of Radiation Medicine, Academy of Military Medical Science, Beijing 100850, China.
authors.forename: Sufang
authors.initials: S
authors.lastname: Shui
authors.affiliation: Institute of Radiation Medicine, Academy of Military Medical Science, Beijing 100850, China; Guangdong Pharmaceutical University, Guangdong 510006, China.
authors.forename: Xiaorong
authors.initials: X
authors.lastname: Cai
authors.affiliation: Anhui Medical University, Hefei 230032, China; Institute of Radiation Medicine, Academy of Military Medical Science, Beijing 100850, China. Electronic address: rqhuang@aliyun.com.
authors.forename: Rongqing
authors.initials: R
authors.lastname: Huang
authors.affiliation: Institute of Radiation Medicine, Academy of Military Medical Science, Beijing 100850, China.
authors.forename: Bingkun
authors.initials: B
authors.lastname: Xiao
authors.affiliation: Institute of Radiation Medicine, Academy of Military Medical Science, Beijing 100850, China.
authors.forename: Jianyun
authors.initials: J
authors.lastname: Yang
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology; D015415:Biomarkers / Q000097:blood; D015232:Dinoprostone / Q000097:blood*; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D008297:Male; D055432:Metabolomics; D051379:Mice; D015398:Signal Transduction / Q000187:drug effects; D014409:Tumor Necrosis Factor-alpha / Q000097:blood*
chemical_list: D000893:Anti-Inflammatory Agents; D015415:Biomarkers; D004365:Drugs, Chinese Herbal; D014409:Tumor Necrosis Factor-alpha; C539461:Yi Guan Jian Decoction; D015232:Dinoprostone
keywords: Anti-inflammation; Metabonomics; Traditional chinese medicine