title: Isolation of Nucleic Acids for Fungal Diagnosis.
abstract: PCR can aid in the diagnosis of invasive fungal disease (IFD). While the large number of "in-house" methodologies drives technological diversity, providing robustness, they make it difficult to identify optimal strategies, limiting standardization, and widespread acceptance. No matter how efficient, PCR utility will be limited by the quality of extracted nucleic acid. This chapter highlights benefits and limitations affecting the nucleic acid extraction process, before focusing on recent recommendations that through multicenter evaluation have provided optimal and standardized methodology.
authors.affiliation: Public Health Wales Microbiology Cardiff, University Hospital of Wales, Heath Park, Cardiff, CF14 4XW, UK. lewis.white@wales.nhs.uk.
authors.forename: P Lewis
authors.initials: PL
authors.lastname: White
authors.affiliation: Public Health Wales Microbiology Cardiff, University Hospital of Wales, Heath Park, Cardiff, CF14 4XW, UK.|Department of Infection and Immunity, Cardiff University, Heath Park, Cardiff, CF14 4XN, UK.
authors.forename: Rosemary A
authors.initials: RA
authors.lastname: Barnes
mesh_terms: D001232:Aspergillus fumigatus / Q000235:genetics; D002176:Candida albicans / Q000235:genetics; D004271:DNA, Fungal / Q000097:blood / Q000302:isolation & purification*; D006801:Humans; D025202:Molecular Diagnostic Techniques; D009181:Mycoses / Q000097:blood / Q000175:diagnosis*; D016133:Polymerase Chain Reaction; D012680:Sensitivity and Specificity
chemical_list: D004271:DNA, Fungal
keywords: DNA extraction; Fungal infection; Sample type