title: Measuring the Kinetic and Mechanical Properties of Non-processive Myosins Using Optical Tweezers.
abstract: The myosin superfamily of molecular motors utilizes energy from ATP hydrolysis to generate force and motility along actin filaments in a diverse array of cellular processes. These motors are structurally, kinetically, and mechanically tuned to their specific molecular roles in the cell. Optical trapping techniques have played a central role in elucidating the mechanisms by which myosins generate force and in exposing the remarkable diversity of myosin functions. Here, we present thorough methods for measuring and analyzing interactions between actin and non-processive myosins using optical trapping techniques.
authors.affiliation: Department of Physiology, The Pennsylvania Muscle Institute, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, 19104, USA. greenberg@wustl.edu.|Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, 660 S. Euclid Ave., Campus Box 8231, St. Louis, MO, 63110, USA. greenberg@wustl.edu.
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Greenberg
authors.affiliation: Department of Physiology, The Pennsylvania Muscle Institute, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, 19104, USA.
authors.forename: Henry
authors.initials: H
authors.lastname: Shuman
authors.affiliation: Department of Physiology, The Pennsylvania Muscle Institute, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, 19104, USA.
authors.forename: E Michael
authors.initials: EM
authors.lastname: Ostap
mesh_terms: D000199:Actins / Q000737:chemistry / Q000378:metabolism; D007700:Kinetics; D020409:Molecular Motor Proteins / Q000737:chemistry* / Q000378:metabolism; D009218:Myosins / Q000737:chemistry* / Q000378:metabolism; D052898:Optical Tweezers; D011485:Protein Binding; D054730:Protein Interaction Domains and Motifs
chemical_list: D000199:Actins; D020409:Molecular Motor Proteins; D009218:Myosins
keywords: Actomyosin; Kinetics; Mechanochemistry; Single molecule