title: Lactobacillus sps. lipase mediated poly (ε-caprolactone) degradation.
abstract: Polymer degradation through lipase appears to be an enthralling alternative to bulk chemical routes. Poly (ε-caprolactone) (PCL) is an artificial polyester that can be degraded by microbes and enzymes like lipases and esterases. The environmental degradation of PCL is dependent on the activity of bacteria that characterization techniques such as thermogravimetric analysis, differential thermal are widely present in the ecosystem. In this study, three different lipases derived from Lactobacillus brevis, Lactobacillus plantarum and their co-culture have been utilized to explore their efficiency towards PCL enzymatic degradation. The effect of parameters such as enzyme loading and degradation time has been explored to understand the efficiency of the enzymes used in this study. Various analysis, scanning electron microscopy and Fourier transform infrared spectroscopy have been employed to study the enzymatic degradation and its possible mechanistic insight.
authors.affiliation: Department of Biological Sciences, BITS Pilani, Hyderabad Campus, Jawahar Nagar, Shameerpet Mandal, Hyderabad 500078, Telangana, India.
authors.forename: Imran
authors.initials: I
authors.lastname: Khan
authors.affiliation: Department of Biological Sciences, BITS Pilani, Hyderabad Campus, Jawahar Nagar, Shameerpet Mandal, Hyderabad 500078, Telangana, India. Electronic address: jayati@hyderabad.bits-pilani.ac.in.
authors.forename: Jayati
authors.initials: J
authors.lastname: Ray Dutta
authors.affiliation: Department of Chemistry, BITS Pilani, Hyderabad Campus, Jawahar Nagar, Shameerpet Mandal, Hyderabad 500078, Telangana, India. Electronic address: ram.ganesan@hyderabad.bits-pilani.ac.in.
authors.forename: Ramakrishnan
authors.initials: R
authors.lastname: Ganesan
mesh_terms: D004795:Enzyme Stability; D007778:Lactobacillus / Q000201:enzymology*; D008049:Lipase / Q000737:chemistry / Q000378:metabolism*; D011091:Polyesters / Q000378:metabolism*; D013696:Temperature
chemical_list: D011091:Polyesters; C016240:polycaprolactone; D008049:Lipase
keywords: Lactobacilllus sps.; Lipase; Poly (ε-caprolactone)