title: A TFPI-1 peptide that induces degradation of bacterial nucleic acids, and inhibits bacterial and viral infection in half-smooth tongue sole, Cynoglossus semilaevis.
abstract: Tissue factor pathway inhibitor 1 (TFPI-1) is a serine protease inhibitor that inhibits tissue factor (TF)-mediated coagulation. The C-terminal region of TFPI-1 could be cleaved off and proved to be antimicrobial against a broad-spectrum of microorganism. In a previous study, a C-terminal peptide, TC24 (with 24 amino acids), derived from tongue sole (Cynoglossus semilaevis) TFPI-1, was synthesized and found antibacterial against Micrococcus luteus. In the present study, the antibacterial spectrum and the action mode of TC24 was further examined, and its in vivo function was analyzed. Our results showed that TC24 also possesses bactericidal activity against Staphylococcus aureus and Vibrio vulnificus. During its interaction with the target bacterial cells, TC24 destroyed cell membrane integrity, penetrated into the cytoplasm, and induced degradation of genomic DNA and total RNA. In vivo study showed that administration of tongue sole with TC24 before bacterial and viral infection significantly reduced pathogen dissemination and replication in tissues. These results indicated that TC24 is a novel antimicrobial peptide against bacterial and viral pathogens, and that the observed effect of TC24 on bacterial RNA adds new insights to the action mechanism of fish antimicrobial peptides. Moreover, TC24 may play an important role in fighting pathogenic infection in aquaculture.
authors.affiliation: Marine Science and Engineering College, Qingdao Agricultural University, Qingdao, 266109, China.
authors.forename: Shu-Wen
authors.initials: SW
authors.lastname: He
authors.affiliation: Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, 266071, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Fishery Drug Development, Ministry of Agriculture, Key Laboratory of Aquatic Animal Immune Technology, Guangdong Province, Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou, 510380, China.
authors.forename: Ning-Qiu
authors.initials: NQ
authors.lastname: Li
authors.affiliation: Marine Science and Engineering College, Qingdao Agricultural University, Qingdao, 266109, China.
authors.forename: Shun
authors.initials: S
authors.lastname: Zhou
authors.affiliation: Marine Science and Engineering College, Qingdao Agricultural University, Qingdao, 266109, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Yue
authors.affiliation: Marine Science and Engineering College, Qingdao Agricultural University, Qingdao, 266109, China. Electronic address: lissazhang06@163.com.
authors.forename: Min
authors.initials: M
authors.lastname: Zhang
mesh_terms: D000818:Animals; D004266:DNA Virus Infections / Q000235:genetics / Q000276:immunology / Q000662:veterinary / Q000821:virology; D005393:Fish Diseases / Q000235:genetics* / Q000276:immunology / Q000382:microbiology / Q000821:virology; D029941:Fish Proteins / Q000235:genetics* / Q000378:metabolism; D005412:Flatfishes; D006090:Gram-Negative Bacteria / Q000502:physiology; D016905:Gram-Negative Bacterial Infections / Q000235:genetics / Q000276:immunology / Q000382:microbiology / Q000662:veterinary; D006094:Gram-Positive Bacteria / Q000502:physiology; D016908:Gram-Positive Bacterial Infections / Q000235:genetics / Q000276:immunology / Q000382:microbiology / Q000662:veterinary; D007497:Iridoviridae / Q000502:physiology; D008074:Lipoproteins / Q000235:genetics* / Q000378:metabolism; D017422:Sequence Analysis, DNA / Q000662:veterinary
chemical_list: D029941:Fish Proteins; D008074:Lipoproteins; C051928:lipoprotein-associated coagulation inhibitor
keywords: Antibacterial mechanism; Antimicrobial peptide; Antiviral; TFPI-1; Teleost