title: Purification, structural characterization and antiproliferative properties of chondroitin sulfate/dermatan sulfate from tunisian fish skins.
abstract: Chondroitin sulfate/dermatan sulfate GAGs were extracted and purified from the skins of grey triggerfish (GTSG) and smooth hound (SHSG). The disaccharide composition produced by chondroitinase ABC treatment showed the presence of nonsulfated disaccharide, monosulfated disaccharides ΔDi6S and ΔDi4S, and disulfated disaccharides in different percentages. In particular, the nonsulfated disaccharide ΔDi0S of GTSG and SHSG were 3.5% and 5.5%, respectively, while monosulfated disaccharides ΔDi6S and ΔDi4S were evaluated to be 18.2%, 59% and 14.6%, 47.0%, respectively. Capillary elecrophoresis analysis of GTSG and SHSG contained 99.2% and 95.4% of chondroitin sulfate/dermatan sulfate, respectively. PAGE analysis showed a GTSG and SHSG having molecular masses with average values of 41.72KDa and 23.8KDa, respectively. HCT116 cell proliferation was inhibited (p<0.05) by 70.6% and 72.65% at 200μg/mL of GTSG and SHSG respectively. Both GTSG and SHSG demonstrated promising antiproliferative potential, which may be used as a novel, effective agent.
authors.affiliation: Laboratoire d'Amélioration des Plantes et Valorisation des Agroressources, Sfax 3038, Université de Sfax, Tunisia.
authors.forename: Fatma
authors.initials: F
authors.lastname: Krichen
authors.affiliation: Department of Life Sciences, University of Modena and Reggio Emilia, Modena, Italy.
authors.forename: Nicola
authors.initials: N
authors.lastname: Volpi
authors.affiliation: Laboratoire d'Amélioration des Plantes et Valorisation des Agroressources, Sfax 3038, Université de Sfax, Tunisia; Institut Régional de Recherche en Agroalimentaire et Biotechnologie: Charles Viollette, EA1026, Equipe ProBioGEM, Université Lille 1, France.
authors.forename: Assaâd
authors.initials: A
authors.lastname: Sila
authors.affiliation: Department of Life Sciences, University of Modena and Reggio Emilia, Modena, Italy.
authors.forename: Francesca
authors.initials: F
authors.lastname: Maccari
authors.affiliation: Department of Life Sciences, University of Modena and Reggio Emilia, Modena, Italy.
authors.forename: Veronica
authors.initials: V
authors.lastname: Mantovani
authors.affiliation: Department of Life Sciences, University of Modena and Reggio Emilia, Modena, Italy.
authors.forename: Fabio
authors.initials: F
authors.lastname: Galeotti
authors.affiliation: Laboratoire d'Amélioration des Plantes et Valorisation des Agroressources, Sfax 3038, Université de Sfax, Tunisia.
authors.forename: Semia
authors.initials: S
authors.lastname: Ellouz-Chaabouni
authors.affiliation: Laboratoire d'Amélioration des Plantes et Valorisation des Agroressources, Sfax 3038, Université de Sfax, Tunisia; Higher Institute of Biotechnology, BP 1175, 3038 Sfax, University of Sfax, Tunisia. Electronic address: ali.bougatef79@gmail.com.
authors.forename: Ali
authors.initials: A
authors.lastname: Bougatef
mesh_terms: D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects; D002809:Chondroitin Sulfates / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology* / Q000633:toxicity; D003871:Dermatan Sulfate / Q000737:chemistry*; D005399:Fishes; D045325:HCT116 Cells; D006461:Hemolysis / Q000187:drug effects; D006801:Humans; D008970:Molecular Weight; D012867:Skin / Q000737:chemistry*
chemical_list: D003871:Dermatan Sulfate; D002809:Chondroitin Sulfates
keywords: Antiproliferative activity; Chondroitin sulfate; Dermatan sulfate; Fish skins; Glycosaminoglycans