title: Comprehensive analysis of epigenetically regulated genes in anergic T cells.
abstract: T cell anergy is one of the important mechanisms for immune tolerance. The results of many studies investigating the mechanism for T cell anergy induction have revealed that the expression of several genes was up-regulated in anergic T cells. It has also been demonstrated that the molecules encoded on those genes played a critical role in anergy induction. However, the mechanism for their up-regulation has not previously been clarified. We examined in this study the changes in gene expression and DNA methylation status caused by anergy induction. Our results demonstrate that the expression of many genes was changed by anergy induction, and that the DNA methylation status of some of these genes was also changed. We show here by a GO analysis that the extent of the change in methylation status caused by anergy induction was distinct between the groups of genes that were categorized.
authors.affiliation: Department of Applied Biological Science, Tokyo University of Agriculture and Technology, Tokyo, Japan.
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Yoshioka
authors.affiliation: Human Resource Development Program in Agricultural Genome Sciences, Tokyo University of Agriculture and Technology, Tokyo, Japan.
authors.forename: Toshinori
authors.initials: T
authors.lastname: Kozaki
authors.affiliation: Human Resource Development Program in Agricultural Genome Sciences, Tokyo University of Agriculture and Technology, Tokyo, Japan.
authors.forename: Kazuo
authors.initials: K
authors.lastname: Ishii
authors.affiliation: Department of Applied Biological Science, Tokyo University of Agriculture and Technology, Tokyo, Japan.
authors.forename: Ayano
authors.initials: A
authors.lastname: Toyoda
authors.affiliation: Department of Applied Biological Science, Tokyo University of Agriculture and Technology, Tokyo, Japan.
authors.forename: Makoto
authors.initials: M
authors.lastname: Hattori
authors.affiliation: Department of Applied Biological Science, Tokyo University of Agriculture and Technology, Tokyo, Japan. Electronic address: tyoshi@cc.tuat.ac.jp.
authors.forename: Tadashi
authors.initials: T
authors.lastname: Yoshida
mesh_terms: D000818:Animals; D017635:Clonal Anergy; D019175:DNA Methylation; D044127:Epigenesis, Genetic; D005786:Gene Expression Regulation; D063990:Gene Ontology; D006801:Humans; D007108:Immune Tolerance; D051379:Mice; D018417:Th1 Cells / Q000276:immunology*; D059467:Transcriptome
keywords: DNA methylation; GO analysis; Next-generation sequencing technology; T cell anergy; Transcriptome