title: A new cerebroside and the cytotoxic constituents isolated from Xylaria allantoidea SWUF76.
abstract: A new cerebroside, namely allantoside (1), and 10 known compounds (2-11) were isolated from Xylaria allantoidea SWUF76. The structure of compound 1 was determined by comprehensive spectroscopic analysis including 1D and 2D nuclear magnetic resonance (NMR) as well as high-resolution electron ionisation mass spectrometry (HREIMS) and electrospray ionisation mass spectrometry (ESIMS). Compounds 1, 4, 5, 6, 7, 8 and 11 were evaluated for cytotoxic activities against cancer cell lines (Hela, HT29, HCT116 and MCF-7) and normal Vero cell lines by MTT assay. Compounds 6 and 7 exhibited anticancer activity after 24 h of treatment. Compound 7 showed significant cytotoxicity against Hela (IC50= 2.24 μg/mL), HT29 (IC50= 2.51 μg/mL), HCT116 (IC50= 3.50 μg/mL) and MCF-7 (IC50= 3.77 μg/mL) and Vero (IC50:3.65 μg/mL) cells. Compound 6 showed slight cytotoxicity against all tested cancer cell lines.
authors.affiliation: a Natural Products Research Unit, Faculty of Science, Department of Chemistry , Khon Kaen University, Centre of Excellence for Innovation in Chemistry (PERCH-CIC) , Khon Kaen , Thailand.
authors.forename: Sirirath
authors.initials: S
authors.lastname: McCloskey
authors.affiliation: a Natural Products Research Unit, Faculty of Science, Department of Chemistry , Khon Kaen University, Centre of Excellence for Innovation in Chemistry (PERCH-CIC) , Khon Kaen , Thailand.
authors.forename: Somchai
authors.initials: S
authors.lastname: Noppawan
authors.affiliation: b Faculty of Science, Department of Microbiology , Khon Kaen University , Khon Kaen , Thailand.
authors.forename: Wiyada
authors.initials: W
authors.lastname: Mongkolthanaruk
authors.affiliation: c Faculty of Science, Department of Biology , Srinakharinwirot University , Bangkok , Thailand.
authors.forename: Nuttika
authors.initials: N
authors.lastname: Suwannasai
authors.affiliation: d Faculty of Science, Department of Biochemistry , Khon Kaen University , Khon Kaen , Thailand.
authors.forename: Thanaset
authors.initials: T
authors.lastname: Senawong
authors.affiliation: e Faculty of Science and Technology, Department of Chemistry , Phuket Rajabhat University , Phuket , Thailand.
authors.forename: Uma
authors.initials: U
authors.lastname: Prawat
mesh_terms: D000970:Antineoplastic Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D045744:Cell Line, Tumor; D002554:Cerebrosides / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D005285:Fermentation; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D013098:Xylariales / Q000378:metabolism*
chemical_list: D000970:Antineoplastic Agents; D002554:Cerebrosides
keywords: Xylaria allantoidea; Xylariaceae; cytotoxicity; secondary metabolite