title: Peripheral Blood Leukocytes Interleukin-1 Beta (IL-1β) Cytokine Hyper-Reactivity in Chronic Periodontitis.
abstract: BACKGROUND Levels of pro-inflammatory cytokine (IL-1β) released by peripheral blood leukocyte medium (PBLM), isolated from chronic periodontitis patients (P) before therapy and matched to controls, were determined in the presence or absence of non-opsonized Escherichia coli and Staphylococcus aureus. MATERIAL AND METHODS In this investigation, 26 patients with untreated, severe, generalized, chronic periodontitis and 26 healthy subjects (H) were enrolled. Periodontal status was assessed by measuring bleeding on probing (BOP), clinical attachment loss (CAL), probing pocket depth (PPD), and Ramfjord index (PDI). The levels of IL-1β (µg/ml) were assayed by a standard Immunoenzymetric Assay Diasource IL-1β ELISA kit in PBLM. RESULTS Our study showed that the values of IL-1β levels in PBLM of the P group (stimulated with non-opsonized E. coli and S. aureus) were significantly higher than in the analogous medium of H group subjects (P<0.001). All correlations between the cytokine levels of IL-1β in the samples of PBLM (stimulated with non-opsonized E. coli and S. aureus) and clinical parameters such as BOP, PPD, CAL, and PDI were significantly higher in the group of patients with periodontitis. CONCLUSIONS Levels of IL-1β secreted by leukocytes may help measure severe, generalized, chronic periodontitis, and can be predictive of future detrimental clinical sequelae associated with chronic periodontitis.
authors.affiliation: Department of Dental and Maxillofacial Orthopedics, Medical Academy, Lithuanian University of Health Sciences, Kaunas, Lithuania.
authors.forename: Jurgina
authors.initials: J
authors.lastname: Sakalauskiene
authors.affiliation: School of Health and Natural Sciences and School of Pharmacy, University of Saint Joseph, West Hartford, CT, USA.
authors.forename: Dalia
authors.initials: D
authors.lastname: Giedrimiene
authors.affiliation: Department of Dental and Maxillofacial Orthopedics, Medical Academy, Lithuanian University of Health Sciences, Kaunas, Lithuania.
authors.forename: Darius
authors.initials: D
authors.lastname: Gleiznys
authors.affiliation: Department of Dental and Maxillofacial Orthopedics, Medical Academy, Lithuanian University of Health Sciences, Kaunas, Lithuania.
authors.forename: Alvydas
authors.initials: A
authors.lastname: Gleiznys
authors.affiliation: Department of Radiology, Medical Academy, Lithuanian University of Health Sciences, Kaunas, Lithuania.
authors.forename: Rymante
authors.initials: R
authors.lastname: Gleizniene
authors.affiliation: Department of Laboratory Medicine, Lithuanian University of Health Sciences, Kaunas, Lithuania.
authors.forename: Astra
authors.initials: A
authors.lastname: Vitkauskiene
mesh_terms: D000328:Adult; D016022:Case-Control Studies; D055113:Chronic Periodontitis / Q000097:blood* / Q000276:immunology / Q000382:microbiology; D016207:Cytokines / Q000097:blood / Q000276:immunology; D004926:Escherichia coli / Q000302:isolation & purification; D005260:Female; D006801:Humans; D053583:Interleukin-1beta / Q000097:blood* / Q000276:immunology; D007962:Leukocytes / Q000276:immunology / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D010512:Periodontal Index; D013211:Staphylococcus aureus / Q000302:isolation & purification
chemical_list: D016207:Cytokines; D053583:Interleukin-1beta
keywords: Leukocytes, Mononuclear; Receptors, Cytokine; chronic periodontitis