title: High-throughput Identification of Bacteria Repellent Polymers for Medical Devices.
abstract: Medical devices are often associated with hospital-acquired infections, which place enormous strain on patients and the healthcare system as well as contributing to antimicrobial resistance. One possible avenue for the reduction of device-associated infections is the identification of bacteria-repellent polymer coatings for these devices, which would prevent bacterial binding at the initial attachment step. A method for the identification of such repellent polymers, based on the parallel screening of hundreds of polymers using a microarray, is described here. This high-throughput method resulted in the identification of a range of promising polymers that resisted binding of various clinically relevant bacterial species individually and also as multi-species communities. One polymer, PA13 (poly(methylmethacrylate-co-dimethylacrylamide)), demonstrated significant reduction in attachment of a number of hospital isolates when coated onto two commercially available central venous catheters. The method described could be applied to identify polymers for a wide range of applications in which modification of bacterial attachment is important.
authors.affiliation: School of Chemistry, EaStCHEM, University of Edinburgh.
authors.forename: Seshasailam
authors.initials: S
authors.lastname: Venkateswaran
authors.affiliation: School of Biological Sciences, University of Edinburgh.
authors.forename: Peter J
authors.initials: PJ
authors.lastname: Gwynne
authors.affiliation: School of Chemistry, EaStCHEM, University of Edinburgh.
authors.forename: Mei
authors.initials: M
authors.lastname: Wu
authors.affiliation: School of Biological Sciences, University of Edinburgh.
authors.forename: Ailsa
authors.initials: A
authors.lastname: Hardman
authors.affiliation: School of Chemistry, EaStCHEM, University of Edinburgh.
authors.forename: Annamaria
authors.initials: A
authors.lastname: Lilienkampf
authors.affiliation: School of Chemistry, EaStCHEM, University of Edinburgh.
authors.forename: Salvatore
authors.initials: S
authors.lastname: Pernagallo
authors.affiliation: School of Biological Sciences, University of Edinburgh.
authors.forename: Garry
authors.initials: G
authors.lastname: Blakely
authors.affiliation: Critical Care, NHS Lothian, Royal Infirmary of Edinburgh.
authors.forename: David G
authors.initials: DG
authors.lastname: Swann
authors.affiliation: School of Chemistry, EaStCHEM, University of Edinburgh; Mark.Bradley@ed.ac.uk.
authors.forename: Mark
authors.initials: M
authors.lastname: Bradley
authors.affiliation: School of Biological Sciences, University of Edinburgh; mp.gallagher@ed.ac.uk.
authors.forename: Maurice P
authors.initials: MP
authors.lastname: Gallagher
mesh_terms: D001419:Bacteria; D001422:Bacterial Adhesion; D004866:Equipment Contamination; D004864:Equipment and Supplies; D011108:Polymers
chemical_list: D011108:Polymers