title: Different antiviral effects of IFNα and IFNβ in an HBV mouse model.
abstract: Interferons α and β (IFNα and IFNβ) are type I interferons produced by the host to control pathogen propagation. However, only a minority of chronic hepatitis B (CHB) patients generate a sustained response after treatment with recombinant IFNα. The anti-HBV effect of IFNβ and the underlying mechanism are not well-understood. Here, we compared the antiviral activities of IFNα and IFNβ by application of IFNα or IFNβ expression plasmids using the well-established HBV hydrodynamic injection (HI) mouse model. Injection of IFNα expression plasmid could significantly reduce HBV serum markers including HBsAg, HBeAg and HBV DNA as well as the number of HBcAg positive cells in the liver, while IFNβ showed only a weak inhibition of HBV replication. In contrast to IFNβ, IFNα resulted in elevated expression levels of IFN stimulated genes (ISGs) as well as the proinflammatory cytokine interleukin 6 (IL6) in the liver. Moreover, IFNβ treated mice showed higher expression levels of the anti-inflammatory cytokines IL10 and TGFβ in the liver compared to IFNα. Our results demonstrated that both IFNα and IFNβ exert antiviral activities against HBV in HI mouse model, but IFNα is more effective than IFNβ.
authors.affiliation: Department of Infectious Diseases, Union Hospital of Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, PR China; School of Medicine, Henan University, Kaifeng, PR China. Electronic address: zyky120@126.com.
authors.forename: Yun
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: Department of Infectious Diseases, Union Hospital of Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, PR China; Experimental Medicine Center, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, PR China. Electronic address: lishengmail@163.com.
authors.forename: Sheng
authors.initials: S
authors.lastname: Li
authors.affiliation: Department of Infectious Diseases, Union Hospital of Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, PR China. Electronic address: tangzongsheng@163.com.
authors.forename: Zongsheng
authors.initials: Z
authors.lastname: Tang
authors.affiliation: Department of Infectious Diseases, Union Hospital of Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, PR China. Electronic address: chunlixuone@yahoo.cn.
authors.forename: Chunli
authors.initials: C
authors.lastname: Xu
authors.affiliation: Department of Infectious Diseases, Union Hospital of Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, PR China. Electronic address: hshmfy2005@163.com.
authors.forename: Shunmei
authors.initials: S
authors.lastname: Huang
authors.affiliation: Department of Infectious Diseases, Union Hospital of Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, PR China. Electronic address: junwu@tjh.tjmu.edu.cn.
authors.forename: Jun
authors.initials: J
authors.lastname: Wu
authors.affiliation: Institute of Virology, University Hospital of Essen, University of Duisburg-Essen, Essen, Germany. Electronic address: ulf.dittmer@uni-due.de.
authors.forename: Ulf
authors.initials: U
authors.lastname: Dittmer
authors.affiliation: Institute of Virology, University Hospital of Essen, University of Duisburg-Essen, Essen, Germany. Electronic address: julia.dickow@uni-due.de.
authors.forename: Julia
authors.initials: J
authors.lastname: Dickow
authors.affiliation: Institute of Virology, University Hospital of Essen, University of Duisburg-Essen, Essen, Germany. Electronic address: Kathrin.sutter@uni-due.de.
authors.forename: Kathrin
authors.initials: K
authors.lastname: Sutter
authors.affiliation: Institute of Virology, University Hospital of Essen, University of Duisburg-Essen, Essen, Germany. Electronic address: mengji.lu@uni-due.de.
authors.forename: Mengji
authors.initials: M
authors.lastname: Lu
authors.affiliation: Department of Infectious Diseases, Union Hospital of Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, PR China. Electronic address: dlyang@hust.edu.cn.
authors.forename: Dongliang
authors.initials: D
authors.lastname: Yang
authors.affiliation: Experimental Medicine Center, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, PR China. Electronic address: jjsong@tjh.tjmu.edu.cn.
authors.forename: Jingjiao
authors.initials: J
authors.lastname: Song
mesh_terms: D000818:Animals; D000998:Antiviral Agents / Q000494:pharmacology; D016207:Cytokines / Q000235:genetics / Q000378:metabolism; D004195:Disease Models, Animal; D015870:Gene Expression; D018014:Gene Transfer Techniques; D006509:Hepatitis B / Q000188:drug therapy / Q000235:genetics* / Q000821:virology*; D006512:Hepatitis B Core Antigens / Q000097:blood / Q000235:genetics; D006515:Hepatitis B virus / Q000145:classification / Q000187:drug effects* / Q000235:genetics; D006801:Humans; D016898:Interferon-alpha / Q000097:blood / Q000235:genetics* / Q000494:pharmacology; D016899:Interferon-beta / Q000097:blood / Q000235:genetics* / Q000494:pharmacology; D008099:Liver / Q000378:metabolism / Q000473:pathology / Q000821:virology; D051379:Mice; D010957:Plasmids / Q000008:administration & dosage / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics; D014162:Transfection; D014452:Ubiquitins / Q000235:genetics / Q000378:metabolism; D019562:Viral Load; D014779:Virus Replication / Q000187:drug effects
chemical_list: D000998:Antiviral Agents; D016207:Cytokines; C489550:G1p2 protein, mouse; D006512:Hepatitis B Core Antigens; D016898:Interferon-alpha; D012333:RNA, Messenger; D014452:Ubiquitins; D016899:Interferon-beta
keywords: Antiviral effect; HBV mouse model; IFNα; IFNβ