title: Synthesis and Functional Characterization of MAF-1A Peptide Derived From the Larvae of Housefly, Musca domestica (Diptera: Muscidae).
abstract: The Musca domestica antifungal peptide-1A (MAF-1A peptide) from housefly larvae was synthesized by solid-phase synthesis technique, and antiviral, antioxidant, and antifungal properties were evaluated in this study. Present results indicated that it could significantly inhibit the infection of influenza virus H1N1, Autographa californica multicapsid nucleopolyhedrovirus (AcMNPV), and Bombyx mori nuclear polyhydrosis virus (BmNPV), which displayed excellent virucidal activities. Antioxidant results demonstrated that the MAF-1A peptide had effective scavenging activity for hydroxyl and superoxide radicals, which were similar to that of ascorbic acid. Besides, antifungal results showed that it can also significantly inhibit the growth of four fungi, and the half inhibitory concentrations (IC50) values were ∼59.3, 84.2, 144.9, and 48.5 μg/ml, respectively, highlighting an important role of MAF-1A peptide in the defense of M domestica against pathogenic microorganisms. These results revealed that the MAF-1A peptide from housefly larvae has great potential as a natural ingredient for the exploitation of antiviral and antifungal therapeutic agents, avoiding abuse of chemical agents and environmental pollution.
authors.affiliation: Hubei Key Laboratory of Genetic Regulation and Integrative Biology, School of Life Sciences, Central China Normal University, Wuhan 430079, China (981733382@qq.com; 1124828343@qq.com; 253165292@qq.com; 15704266@qq.com; aihui@mail.ccnu.edu.cn).
authors.forename: Jing
authors.initials: J
authors.lastname: Zhou
authors.affiliation: Hubei Key Laboratory of Genetic Regulation and Integrative Biology, School of Life Sciences, Central China Normal University, Wuhan 430079, China (981733382@qq.com; 1124828343@qq.com; 253165292@qq.com; 15704266@qq.com; aihui@mail.ccnu.edu.cn).
authors.forename: Lina
authors.initials: L
authors.lastname: Kong
authors.affiliation: Hubei Key Laboratory of Genetic Regulation and Integrative Biology, School of Life Sciences, Central China Normal University, Wuhan 430079, China (981733382@qq.com; 1124828343@qq.com; 253165292@qq.com; 15704266@qq.com; aihui@mail.ccnu.edu.cn).
authors.forename: Nainai
authors.initials: N
authors.lastname: Fang
authors.affiliation: Hubei Key Laboratory of Genetic Regulation and Integrative Biology, School of Life Sciences, Central China Normal University, Wuhan 430079, China (981733382@qq.com; 1124828343@qq.com; 253165292@qq.com; 15704266@qq.com; aihui@mail.ccnu.edu.cn).
authors.forename: Bin
authors.initials: B
authors.lastname: Mao
authors.affiliation: Hubei Key Laboratory of Genetic Regulation and Integrative Biology, School of Life Sciences, Central China Normal University, Wuhan 430079, China (981733382@qq.com; 1124828343@qq.com; 253165292@qq.com; 15704266@qq.com; aihui@mail.ccnu.edu.cn) aihui@mail.ccnu.edu.cn.
authors.forename: Hui
authors.initials: H
authors.lastname: Ai
mesh_terms: D000818:Animals; D000935:Antifungal Agents / Q000032:analysis / Q000138:chemical synthesis / Q000494:pharmacology*; D000975:Antioxidants / Q000032:analysis / Q000138:chemical synthesis / Q000494:pharmacology*; D000998:Antiviral Agents / Q000032:analysis / Q000138:chemical synthesis / Q000494:pharmacology*; D004285:Dogs; D005658:Fungi / Q000187:drug effects; D006793:Houseflies / Q000737:chemistry* / Q000254:growth & development; D053118:Influenza A Virus, H1N1 Subtype / Q000187:drug effects; D019476:Insect Proteins / Q000032:analysis / Q000138:chemical synthesis / Q000494:pharmacology*; D007814:Larva / Q000737:chemistry; D061985:Madin Darby Canine Kidney Cells; D017924:Nucleopolyhedroviruses / Q000187:drug effects; D061987:Sf9 Cells
chemical_list: D000935:Antifungal Agents; D000975:Antioxidants; D000998:Antiviral Agents; D019476:Insect Proteins
keywords: MAF-1A peptide; Musca domestica L.; antifungal activity; antioxidant activity; antiviral activity