title: Genetic determination of the enhanced drought resistance of rice maintainer HuHan2B by pedigree breeding.
abstract: The ongoing deficit of fresh water resource in rice growing regions has made the selection of water-saving and drought-resistance rice (WDR) a crucial factor in developing sustainable cultivation. HuHan2B, a new japonica maintainer for WDR breeding, had the same yield potential as recurrent parent HanFengB but showed improved drought resistance in fields. We investigated the genomic content accumulation and candidate genes passed from parent to offspring using the genomic and transcriptomic approaches. The genomic constitution indicated that the genetic similarity was 84% between HuHan2B and HanFengB; additionally, 7,256 genes with specific alleles were inherited by HuHan2B from parents other than HanFengB. The differentially expressed genes (DEGs) under drought stress showed that biological function was significantly enriched for transcript regulation in HuHan2B, while the oxidation-reduction process was primarily enriched in HanFengB. Furthermore, 36 DEGs with specific inherited alleles in HuHan2B were almost involved in the regulatory network of TFs and target genes. These findings suggested that major-effect genes were congregated and transformed into offspring in manner of interacting network by breeding. Thus, exploiting the potential biological function of allelic-influencing DEGs would be of great importance for improving selection efficiency and the overall genetic gain of multiple complex traits.
authors.affiliation: Shanghai Agrobiological Gene Center, Shanghai 201106, China.
authors.forename: Haibin
authors.initials: H
authors.lastname: Wei
authors.affiliation: Shanghai Agrobiological Gene Center, Shanghai 201106, China.
authors.forename: Fangjun
authors.initials: F
authors.lastname: Feng
authors.affiliation: Shanghai Agrobiological Gene Center, Shanghai 201106, China.
authors.forename: Qiaojun
authors.initials: Q
authors.lastname: Lou
authors.affiliation: Shanghai Agrobiological Gene Center, Shanghai 201106, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Xia
authors.affiliation: Shanghai Agrobiological Gene Center, Shanghai 201106, China.
authors.forename: Xiaosong
authors.initials: X
authors.lastname: Ma
authors.affiliation: Shanghai Agrobiological Gene Center, Shanghai 201106, China.
authors.forename: Yunhua
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Shanghai Agrobiological Gene Center, Shanghai 201106, China.
authors.forename: Kai
authors.initials: K
authors.lastname: Xu
authors.affiliation: Shanghai Agrobiological Gene Center, Shanghai 201106, China.
authors.forename: Xinqiao
authors.initials: X
authors.lastname: Yu
authors.affiliation: Shanghai Agrobiological Gene Center, Shanghai 201106, China.
authors.forename: Hanwei
authors.initials: H
authors.lastname: Mei
authors.affiliation: Shanghai Agrobiological Gene Center, Shanghai 201106, China.
authors.forename: Lijun
authors.initials: L
authors.lastname: Luo
mesh_terms: D000222:Adaptation, Physiological; D003681:Dehydration / Q000235:genetics; D055864:Droughts; D053263:Gene Regulatory Networks; D018745:Genome, Plant; D012275:Oryza / Q000235:genetics*; D010375:Pedigree; D000069600:Plant Breeding; D010940:Plant Proteins / Q000235:genetics; D020641:Polymorphism, Single Nucleotide; D017422:Sequence Analysis, DNA; D014157:Transcription Factors / Q000235:genetics; D059467:Transcriptome
chemical_list: D010940:Plant Proteins; D014157:Transcription Factors