title: Enhancement of Satellite Cell Transplantation Efficiency by Leukemia Inhibitory Factor.
abstract: Cell transplantation is a promising therapy for several muscle diseases, including Duchenne muscular dystrophy. Satellite cells are stem cells in skeletal muscle that provide an important cell source for transplantation therapy. However, culture of satellite cells in vitro causes them to lose their undifferentiated state, associated with reduced transplantation efficiency. It is therefore necessary to develop optimal culture conditions for maintaining the undifferentiated state of satellite cells.
  
  
  
  Primary satellite cells were cultured with or without leukemia inhibitory factor (LIF). The expression of undifferentiation and differentiation markers, and the transplantation efficiency were analyzed.
  
  
  
  LIF-treated satellite cells showed increased expression of Pax7, and enhanced transplantation efficiency in a mouse model of Duchenne muscular dystrophy.
  
  
  
  Our study showed that the treatment with LIF effectively maintained the undifferentiated state of satellite cells, and enhanced their transplantation efficiency. These results will contribute to the optimization of culture conditions for cell transplantation therapy.
authors.affiliation: Department of Molecular Therapy, National Institute of Neuroscience, National Center of Neurology and Psychiatry, Kodaira, Japan.|Department of Rheumatology and Allergy, IMSUT Hospital, The Institute of Medical Science, The University of Tokyo, Shirokanedai, Minato-ku, Tokyo, Japan.
authors.forename: N
authors.initials: N
authors.lastname: Ito
authors.affiliation: Division of Rheumatology, Center for Antibody and Vaccine, IMSUT Hospital, The Institute of Medical Science, The University of Tokyo, Shirokanedai, Minato-ku, Tokyo, Japan.
authors.forename: N
authors.initials: N
authors.lastname: Shimizu
authors.affiliation: Department of Rheumatology and Allergy, IMSUT Hospital, The Institute of Medical Science, The University of Tokyo, Shirokanedai, Minato-ku, Tokyo, Japan.|Division of Rheumatology, Center for Antibody and Vaccine, IMSUT Hospital, The Institute of Medical Science, The University of Tokyo, Shirokanedai, Minato-ku, Tokyo, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Tanaka
authors.affiliation: Department of Molecular Therapy, National Institute of Neuroscience, National Center of Neurology and Psychiatry, Kodaira, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Takeda
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques; D002454:Cell Differentiation / Q000187:drug effects*; D053605:Leukemia Inhibitory Factor / Q000494:pharmacology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D016688:Mice, Inbred NOD; D016513:Mice, SCID; D018482:Muscle, Skeletal / Q000166:cytology*; D020388:Muscular Dystrophy, Duchenne / Q000628:therapy; D032496:Satellite Cells, Skeletal Muscle / Q000187:drug effects* / Q000637:transplantation
chemical_list: D053605:Leukemia Inhibitory Factor
keywords: Satellite cell; cell transplantation therapy; leukemia inhibitory factor; muscular dystrophy