title: Chlorogenic Acid Combined with Lactobacillus plantarum 2142 Reduced LPS-Induced Intestinal Inflammation and Oxidative Stress in IPEC-J2 Cells.
abstract: This study was carried out to investigate protective effect of chlorogenic acid against lipopolysaccharide-induced inflammation and oxidative stress in intestinal epithelial cells. As a marker of inflammatory response, IL-6, IL-8, TNF-α mRNA and protein levels, furthermore, COX-2 mRNA level were followed up. Intracellular redox status and extracellular H2O2 level were also monitored by two fluorescent assays (DCFH-DA, Amplex Red). Moreover, the effect of gut microbiota metabolites in the above mentioned processes was taken into account in our model using Lactobacillus plantarum 2142 bacterial strain. Our data revealed that chlorogenic acid had significant lowering effect on the inflammatory response. Treatment with chlorogenic acid (25-50 μM) significantly decreased gene expression and concentration of proinflammatory cytokines IL-6 and IL-8 compared to LPS-treated cells. COX-2 and TNF-α mRNA levels were also reduced. Furthermore, chlorogenic acid reduced the level of reactive oxygen species in IPEC-J2 cells. Simultaneous application of chlorogenic acid and Lactobacillus plantarum 2142 supernatant resulted protective effect against LPS-induced inflammation and oxidative stress as well.
authors.affiliation: Department of Pharmacology and Toxicology, University of Veterinary Medicine, Budapest, Hungary.
authors.forename: Orsolya
authors.initials: O
authors.lastname: Palócz
authors.affiliation: Department of Pharmacology and Toxicology, University of Veterinary Medicine, Budapest, Hungary.
authors.forename: Erzsébet
authors.initials: E
authors.lastname: Pászti-Gere
authors.affiliation: Department of Pharmacology and Toxicology, University of Veterinary Medicine, Budapest, Hungary.
authors.forename: Péter
authors.initials: P
authors.lastname: Gálfi
authors.affiliation: Department of Pharmacology and Toxicology, University of Veterinary Medicine, Budapest, Hungary.
authors.forename: Orsolya
authors.identifier: http://orcid.org/0000-0002-0828-7902
authors.initials: O
authors.lastname: Farkas
mesh_terms: D000818:Animals; D002460:Cell Line; D002470:Cell Survival; D002726:Chlorogenic Acid / Q000737:chemistry / Q000494:pharmacology*; D016207:Cytokines / Q000378:metabolism; D004847:Epithelial Cells / Q000187:drug effects / Q000378:metabolism; D005759:Gastroenteritis / Q000209:etiology* / Q000378:metabolism* / Q000628:therapy; D018836:Inflammation Mediators / Q000378:metabolism; D007413:Intestinal Mucosa / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D048191:Lactiplantibacillus plantarum / Q000502:physiology*; D008070:Lipopolysaccharides / Q000009:adverse effects; D010084:Oxidation-Reduction / Q000187:drug effects; D018384:Oxidative Stress / Q000187:drug effects*; D019936:Probiotics / Q000627:therapeutic use; D020011:Protective Agents / Q000494:pharmacology*; D017382:Reactive Oxygen Species / Q000378:metabolism; D013552:Swine
chemical_list: D016207:Cytokines; D018836:Inflammation Mediators; D008070:Lipopolysaccharides; D020011:Protective Agents; D017382:Reactive Oxygen Species; D002726:Chlorogenic Acid