title: Endometriotic cyst fluid induces reactive oxygen species (ROS) in human immortalized epithelial cells derived from ovarian endometrioma.
abstract: OBJECTIVE
  Endometriotic cyst fluid (ECF) contains a large amount of reactive oxygen species (ROS), and endometriotic cysts are exposed to strong oxidative stress, which may cause malignant transformation. In this study, ROS production by ECF was clinically analysed.
  
  
  METHODS
  Human immortalized epithelial cells derived from ovarian endometrioma (EMosis-CC/TERT 1) were treated with ECF. In addition, ROS production in EMosis-CC/TERT 1 was measured, and its clinical significance was analysed.
  
  
  RESULTS
  A total of 38 ECF samples were obtained from patients diagnosed with endometriotic cysts. In EMosis-CC/TERT1, significantly higher levels of ROS were induced by ECF than by the vehicle control and ferric nitrilotriacetate. There were no significant differences in ROS production by laterality and preoperative serum CA125 values. There were several patients whose cyst sizes were approximately 5 cm and had relatively high ROS production. Production of ROS by ECF was relatively higher in patients older than 40 years of age than in those younger than 40.
  
  
  CONCLUSIONS
  Our study revealed that ROS are highly produced by ECF in EMosis-CC/TERT1 cells; therefore, exposure to ECF induced strong oxidative stress. Development of a therapeutic strategy to reduce ROS production might be useful for preventing malignant transformation of endometriotic cysts.
authors.affiliation: a Department of Obstetrics and Gynecology , Kyorin University School of Medicine , Mitaka , Tokyo , Japan.
authors.forename: Yoichi
authors.initials: Y
authors.lastname: Kobayashi
authors.affiliation: a Department of Obstetrics and Gynecology , Kyorin University School of Medicine , Mitaka , Tokyo , Japan.
authors.forename: Kiyono
authors.initials: K
authors.lastname: Osanai
authors.affiliation: a Department of Obstetrics and Gynecology , Kyorin University School of Medicine , Mitaka , Tokyo , Japan.
authors.forename: Kei
authors.initials: K
authors.lastname: Tanaka
authors.affiliation: a Department of Obstetrics and Gynecology , Kyorin University School of Medicine , Mitaka , Tokyo , Japan.
authors.forename: Yoshiko
authors.initials: Y
authors.lastname: Nishigaya
authors.affiliation: a Department of Obstetrics and Gynecology , Kyorin University School of Medicine , Mitaka , Tokyo , Japan.
authors.forename: Hironori
authors.initials: H
authors.lastname: Matsumoto
authors.affiliation: a Department of Obstetrics and Gynecology , Kyorin University School of Medicine , Mitaka , Tokyo , Japan.
authors.forename: Mai
authors.initials: M
authors.lastname: Momomura
authors.affiliation: b Development Research , Mochida Pharmaceutical Co., Ltd. , Gotemba , Shizuoka , Japan.
authors.forename: Masamichi
authors.initials: M
authors.lastname: Hashiba
authors.affiliation: b Development Research , Mochida Pharmaceutical Co., Ltd. , Gotemba , Shizuoka , Japan.
authors.forename: Shizuka
authors.identifier: http://orcid.org/0000-0001-7626-1862
authors.initials: S
authors.lastname: Mita
authors.affiliation: c Department of Obstetrics and Gynecology , Shimane University School of Medicine , Izumo , Shimane , Japan.
authors.forename: Satoru
authors.initials: S
authors.lastname: Kyo
authors.affiliation: a Department of Obstetrics and Gynecology , Kyorin University School of Medicine , Mitaka , Tokyo , Japan.
authors.forename: Mitsutoshi
authors.initials: M
authors.lastname: Iwashita
mesh_terms: D000328:Adult; D018394:CA-125 Antigen / Q000097:blood; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D020420:Cyst Fluid / Q000378:metabolism*; D004715:Endometriosis / Q000097:blood / Q000378:metabolism*; D004847:Epithelial Cells / Q000187:drug effects* / Q000378:metabolism*; D005260:Female; D006801:Humans; D008565:Membrane Proteins / Q000097:blood; D018384:Oxidative Stress / Q000187:drug effects; D017382:Reactive Oxygen Species / Q000378:metabolism*
chemical_list: D018394:CA-125 Antigen; C451759:MUC16 protein, human; D008565:Membrane Proteins; D017382:Reactive Oxygen Species
keywords: Endometriotic cyst fluid; endometrioma; oxidative stress; reactive oxygen species