title: Chondrocytes from congenital microtia possess an inferior capacity for in vivo cartilage regeneration to healthy ear chondrocytes.
abstract: The remnant auricular cartilage from microtia has become a valuable cell source for ear regeneration. It is important to clarify the issue of whether the genetically defective microtia chondrocytes could engineer cartilage tissue comparable to healthy ear chondrocytes. In the current study, the histology and cell yield of native microtia and normal ear cartilage were investigated, and the biological characteristics of derived chondrocytes examined, including proliferation, chondrogenic phenotype and cell migration. Furthermore, the in vivo cartilage-forming capacity of passaged microtia and normal auricular chondrocytes were systematically compared by seeding them onto polyglycolic acid/polylactic acid scaffold to generate tissue engineered cartilage in nude mice. Through histological examinations and quantitative analysis of glycosaminoglycan, Young's modulus, and the expression of cartilage-related genes, it was found that microtia chondrocytes had a slower dedifferentiation rate with the decreased expression of stemness-related genes, and weaker migration ability than normal ear chondrocytes, and the microtia chondrocytes-engineered cartilage was biochemically and biomechanically inferior to that constructed using normal ear chondrocytes. This study provides valuable information for the clinical application of the chondrocytes derived from congenital microtia to engineer cartilage. Copyright © 2016 John Wiley & Sons, Ltd.
authors.affiliation: Research Center of Plastic Surgery Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, P. R. China.
authors.forename: Yunpeng
authors.initials: Y
authors.lastname: Gu
authors.affiliation: Research Center of Plastic Surgery Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, P. R. China.
authors.forename: Ning
authors.initials: N
authors.lastname: Kang
authors.affiliation: Research Center of Plastic Surgery Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, P. R. China.
authors.forename: Ping
authors.initials: P
authors.lastname: Dong
authors.affiliation: Research Center of Plastic Surgery Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, P. R. China.
authors.forename: Xia
authors.initials: X
authors.lastname: Liu
authors.affiliation: Research Center of Plastic Surgery Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, P. R. China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Wang
authors.affiliation: Research Center of Plastic Surgery Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, P. R. China.
authors.forename: Xin
authors.initials: X
authors.lastname: Fu
authors.affiliation: Research Center of Plastic Surgery Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, P. R. China.
authors.forename: Li
authors.initials: L
authors.lastname: Yan
authors.affiliation: Research Center of Plastic Surgery Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, P. R. China.
authors.forename: Haiyue
authors.initials: H
authors.lastname: Jiang
authors.affiliation: Research Center of Plastic Surgery Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, P. R. China.
authors.forename: Yilin
authors.initials: Y
authors.lastname: Cao
authors.affiliation: Research Center of Plastic Surgery Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, P. R. China.
authors.forename: Ran
authors.initials: R
authors.lastname: Xiao
mesh_terms: D000293:Adolescent; D000328:Adult; D002465:Cell Movement; D049109:Cell Proliferation; D002648:Child; D019902:Chondrocytes / Q000378:metabolism / Q000473:pathology*; D020219:Chondrogenesis / Q000235:genetics; D065817:Congenital Microtia / Q000473:pathology*; D004425:Ear Cartilage / Q000503:physiopathology*; D005786:Gene Expression Regulation; D006025:Glycosaminoglycans / Q000378:metabolism; D006801:Humans; D010641:Phenotype; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012038:Regeneration; D023822:Tissue Engineering; D055815:Young Adult
chemical_list: D006025:Glycosaminoglycans; D012333:RNA, Messenger
keywords: auricular chondrocytes; cartilage tissue engineering; dedifferentiation; ear regeneration; microtia