title: Insights into structure and function of 30S Ribosomal Protein S2 (30S2) in Chlamydophila pneumoniae: A potent target of pneumonia.
abstract: The gene 30S ribosomal protein S2 (30S2) is identified as a potential drug and vaccine target for Pneumonia. Its structural characterization is an important to understand the mechanism of action for identifying its receptor and/or other binding partners. The comparative genomics and proteomics studies are useful for structural characterization of 30S2 in C. Pneumoniae using different bioinformatics tools and web servers. In this study, the protein 30S2 structure was modelled and validated by Ramachandran plot. It is found that the modelled protein under most favoured "core" region was 88.7% and overall G-factor statistics with average score was -0.20. However, seven sequential motifs have been identified for 30S2 with reference codes (PR0095, PF0038, TIGR01012, PTHR11489, SSF52313 and PTHR11489). In addition, seven structural highly conserved residues have been identified in the large cleft are Lys160, Gly161and Arg162 with volume 1288.83Å3and average depth of the cleft was 10.75Å. Moreover, biological functions, biochemical process and structural constituents of ribosome are also explored. The study will be helped us to understand the sequential, structural, functional and evolutionary clues of unknown proteins available in C. Pneumoniae.
authors.affiliation: Centre for Disaster Mitigation and Management, VIT University, Vellore-632014, India. Electronic address: kotireddy.nit@gmail.com.
authors.forename: G
authors.initials: G
authors.lastname: Koteswara Reddy
authors.affiliation: School of Civil and Chemical Engineering, VIT University, Vellore-632014, India.
authors.forename: K
authors.initials: K
authors.lastname: Nagamalleswara Rao
authors.affiliation: Centre for Disaster Mitigation and Management, VIT University, Vellore-632014, India.
authors.forename: Kiran
authors.initials: K
authors.lastname: Yarrakula
mesh_terms: D000595:Amino Acid Sequence; D001665:Binding Sites; D016993:Chlamydophila pneumoniae / Q000737:chemistry*; D004247:DNA / Q000378:metabolism; D008958:Models, Molecular; D018410:Pneumonia, Bacterial / Q000378:metabolism*; D011487:Protein Conformation; D012269:Ribosomal Proteins / Q000737:chemistry* / Q000378:metabolism*; D017386:Sequence Homology, Amino Acid
chemical_list: D012269:Ribosomal Proteins; C030004:ribosomal protein S2; C040469:ribosomal protein S30; D004247:DNA
keywords: 30S ribosomal protein S2; Chlamydophila pneumoniae; Drug target; Function; Pneumonia; Profunc server; Ramachandran plot; Structure