title: The cysteine residues at the C-terminal tail of Bamboo mosaic virus triple gene block protein 2 are critical for efficient plasmodesmata localization of protein 1 in the same block.
abstract: The movement of some plant viruses are accomplished by three proteins encoded by a triple gene block (TGB). The second protein (TGBp2) in the block is a transmembrane protein. This study was aimed to unravel the mechanism underlying the relatively inefficient cell-to-cell movement of Bamboo mosaic virus (BaMV) caused by amino acid substitutions for the three Cys residues, Cys-109, Cys-112 and Cys-119, at the C-terminal tail of TGBp2. Results from confocal microscopy revealed that substitutions of the three Cys residues of TGBp2, especially Cys-109 and Cys-112, would reduce the efficiency of TGBp2- and TGBp3-dependent PD localization of TGBp1. Moreover, there is an additive effect of the substitutions on reducing the efficiency of PD localization of TGBp1. These results indicate that the Cys residues in the C-terminal tail region of TGBp2 participate in the TGBp2- and TGBp3-dependent PD localization of TGBp1, and thus influence the cell-to-cell movement capability of BaMV.
authors.affiliation: Institute of Biochemistry, National Chung-Hsing University, Taichung 40227, Taiwan, Republic of China.
authors.forename: Tsai-Ling
authors.initials: TL
authors.lastname: Ho
authors.affiliation: Graduate Institute of Biotechnology, National Chung-Hsing University, Taichung 40227, Taiwan, Republic of China; Ph.D. Program in Microbial Genomics, National Chung-Hsing University and Academia Sinica, Taiwan, Republic of China.
authors.forename: Hsiang-Chi
authors.initials: HC
authors.lastname: Lee
authors.affiliation: Institute of Biochemistry, National Chung-Hsing University, Taichung 40227, Taiwan, Republic of China.
authors.forename: Yuan-Lin
authors.initials: YL
authors.lastname: Chou
authors.affiliation: Institute of Biochemistry, National Chung-Hsing University, Taichung 40227, Taiwan, Republic of China.
authors.forename: Yang-Hao
authors.initials: YH
authors.lastname: Tseng
authors.affiliation: Institute of Biochemistry, National Chung-Hsing University, Taichung 40227, Taiwan, Republic of China.
authors.forename: Wei-Cheng
authors.initials: WC
authors.lastname: Huang
authors.affiliation: Biotechnology Center, National Chung-Hsing University, Taichung, Taiwan, Republic of China.
authors.forename: Chiung-Hua
authors.initials: CH
authors.lastname: Wung
authors.affiliation: Graduate Institute of Biotechnology, National Chung-Hsing University, Taichung 40227, Taiwan, Republic of China; Institute of Plant and Microbial Biology, Academia Sinica, Taipei, Taiwan, Republic of China.
authors.forename: Na-Sheng
authors.initials: NS
authors.lastname: Lin
authors.affiliation: Graduate Institute of Biotechnology, National Chung-Hsing University, Taichung 40227, Taiwan, Republic of China.
authors.forename: Yau-Heiu
authors.initials: YH
authors.lastname: Hsu
authors.affiliation: Institute of Biochemistry, National Chung-Hsing University, Taichung 40227, Taiwan, Republic of China. Electronic address: bychang@mail.nchu.edu.tw.
authors.forename: Ban-Yang
authors.initials: BY
authors.lastname: Chang
mesh_terms: D020816:Amino Acid Motifs; D003545:Cysteine / Q000235:genetics* / Q000378:metabolism; D010935:Plant Diseases / Q000821:virology*; D031425:Plasmodesmata / Q000378:metabolism / Q000821:virology*; D017863:Potexvirus / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D021381:Protein Transport; D014026:Nicotiana / Q000821:virology*; D014764:Viral Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*
chemical_list: D014764:Viral Proteins; D003545:Cysteine
keywords: Bamboo mosaic virus; Cell-to-cell movement; Plasmodesmata targeting; Triple gene block proteins