title: Highly Sensitive and Practical Detection of Plant Viruses via Electrical Impedance of Droplets on Textured Silicon-Based Devices.
abstract: Early diagnosis of plant virus infections before the disease symptoms appearance may represent a significant benefit in limiting disease spread by a prompt application of appropriate containment steps. We propose a label-free procedure applied on a device structure where the electrical signal transduction is evaluated via impedance spectroscopy techniques. The device consists of a droplet suspension embedding two representative purified plant viruses i.e.,Tomato mosaic virusandTurnip yellow mosaic virus, put in contact with a highly hydrophobic plasma textured silicon surface. Results show a high sensitivity of the system towards the virus particles with an interestingly low detection limit, from tens to hundreds of attomolar corresponding to pg/mL of sap, which refers, in the infection time-scale, to a concentration of virus particles in still-symptomless plants. Such a threshold limit, together with an envisaged engineering of an easily manageable device, compared to more sophisticated apparatuses, may contribute in simplifying the in-field plant virus diagnostics.
authors.affiliation: CNR NANOTEC-Istituto di Nanotecnologia, Via Amendola 122, Bari 70126, Italy. marianna.ambrico@cnr.it.
authors.forename: Marianna
authors.initials: M
authors.lastname: Ambrico
authors.affiliation: CNR NANOTEC-Istituto di Nanotecnologia, Via Amendola 122, Bari 70126, Italy. paolofrancesco.ambrico@cnr.it.
authors.forename: Paolo Francesco
authors.initials: PF
authors.lastname: Ambrico
authors.affiliation: CNR IPSP-Istituto per la Protezione Sostenibile delle Piante, UoS Bari, Via Amendola 165, Bari 70126, Italy. angelantonio.minafra@ipsp.cnr.it.
authors.forename: Angelantonio
authors.initials: A
authors.lastname: Minafra
authors.affiliation: CNR IPSP-Istituto per la Protezione Sostenibile delle Piante, UoS Bari, Via Amendola 165, Bari 70126, Italy. angelo.destradis@ipsp.cnr.it.
authors.forename: Angelo
authors.initials: A
authors.lastname: De Stradis
authors.affiliation: Dipartimento di Chimica, Università degli Studi di Bari, Via Orabona 4, Bari 70125, Italy. danilo.vona@gmail.com.
authors.forename: Danilo
authors.initials: D
authors.lastname: Vona
authors.affiliation: CNR ICCOM-Istituto di Chimica dei Composti OrganoMetallici, Via Orabona 4, Bari 70125, Italy. cicco@ba.iccom.cnr.it.
authors.forename: Stefania R
authors.initials: SR
authors.lastname: Cicco
authors.affiliation: CNR NANOTEC-Istituto di Nanotecnologia, Via Amendola 122, Bari 70126, Italy. fabio.palumbo@cnr.it.
authors.forename: Fabio
authors.initials: F
authors.lastname: Palumbo
authors.affiliation: Dipartimento di Chimica, Università degli Studi di Bari, Via Orabona 4, Bari 70125, Italy. pietro.favia@uniba.it.
authors.forename: Pietro
authors.initials: P
authors.lastname: Favia
authors.affiliation: Dipartimento Interateneo di Fisica, Università degli Studi di Bari, Via Orabona 4, Bari 70125, Italy. ligonzo@fisica.uniba.it.
authors.forename: Teresa
authors.initials: T
authors.lastname: Ligonzo
mesh_terms: D017097:Electric Impedance; D057927:Hydrophobic and Hydrophilic Interactions; D010942:Plant Viruses / Q000378:metabolism*; D012825:Silicon / Q000737:chemistry*; D017898:Tobamovirus / Q000378:metabolism
chemical_list: D012825:Silicon
keywords: EIS; TYMV; ToMV; droplet-based device; label-free detection; plant viruses; surface texturing