title: Biochemical characterization of the medaka (Oryzias latipes) orthologue for mammalian tissue-type transglutaminase (TG2).
abstract: Transglutaminase is an enzyme family responsible for post-translational modification such as protein cross-linking and the attachment of primary amine and/or deamidation of glutamine-residue in proteins. Medaka (Oryzias latipes), a recently established model fish, has similar functional proteins to those characterized in mammals. Previously, we found the apparent orthologues that correspond to human transglutaminases in medaka. In this study, regarding the medaka orthologue of human tissue-type transglutaminase (OlTGT), recombinant protein was expressed in an active form in bacteria cultured at low temperature. Using the recombinant protein, we biochemically characterized the enzymatic activity and also obtained a monoclonal antibody that specifically recognized OlTGT. Immunochemical analysis revealed that OlTGT was not expressed ubiquitously, unlike its mammalian orthologue, but in primarily limited tissues such as the eye, brain, spinal cord, and gas gland.
authors.affiliation: a Graduate School of Pharmaceutical Sciences , Nagoya University , Nagoya , Japan.
authors.forename: Yuki
authors.initials: Y
authors.lastname: Takada
authors.affiliation: a Graduate School of Pharmaceutical Sciences , Nagoya University , Nagoya , Japan.
authors.forename: Yuko
authors.initials: Y
authors.lastname: Watanabe
authors.affiliation: a Graduate School of Pharmaceutical Sciences , Nagoya University , Nagoya , Japan.
authors.forename: Kazuho
authors.initials: K
authors.lastname: Okuya
authors.affiliation: a Graduate School of Pharmaceutical Sciences , Nagoya University , Nagoya , Japan.
authors.forename: Hideki
authors.initials: H
authors.lastname: Tatsukawa
authors.affiliation: b Bioscience Biotechnology Center, Nagoya University , Nagoya , Japan.
authors.forename: Hisashi
authors.initials: H
authors.lastname: Hashimoto
authors.affiliation: a Graduate School of Pharmaceutical Sciences , Nagoya University , Nagoya , Japan.
authors.forename: Kiyotaka
authors.initials: K
authors.lastname: Hitomi
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D029941:Fish Proteins / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D019204:GTP-Binding Proteins / Q000737:chemistry* / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D009990:Oryzias / Q000378:metabolism*; D000091345:Protein Glutamine gamma Glutamyltransferase 2; D017208:Rats, Wistar; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D011503:Transglutaminases / Q000737:chemistry* / Q000235:genetics / Q000276:immunology / Q000378:metabolism*
chemical_list: D000911:Antibodies, Monoclonal; D029941:Fish Proteins; D011994:Recombinant Proteins; C000717609:Tgm2 protein, rat; D000091345:Protein Glutamine gamma Glutamyltransferase 2; D011503:Transglutaminases; D019204:GTP-Binding Proteins
keywords: Oryzias latipes; calcium; transglutaminase