title: Mechanisms for the control of local tissue blood flow during thermal interventions: influence of temperature-dependent ATP release from human blood and endothelial cells.
abstract: What is the central question of this study? Skin and muscle blood flow increases with heating and decreases with cooling, but the temperature-sensitive mechanisms underlying these responses are not fully elucidated. What is the main finding and its importance? We found that local tissue hyperaemia was related to elevations in ATP release from erythrocytes. Increasing intravascular ATP augmented skin and tissue perfusion to levels equal or above thermal hyperaemia. ATP release from isolated erythrocytes was altered by heating and cooling. Our findings suggest that erythrocytes are involved in thermal regulation of blood flow via modulation of ATP release. Local tissue perfusion changes with alterations in temperature during heating and cooling, but the thermosensitivity of the vascular ATP signalling mechanisms for control of blood flow during thermal interventions remains unknown. Here, we tested the hypotheses that the release of the vasodilator mediator ATP from human erythrocytes, but not from endothelial cells or other blood constituents, is sensitive to both increases and reductions in temperature and that increasing intravascular ATP availability with ATP infusion would potentiate thermal hyperaemia in limb tissues. We first measured blood temperature, brachial artery blood flow and plasma [ATP] during passive arm heating and cooling in healthy men and found that they increased by 3.0 ± 1.2°C, 105 ± 25 ml min-1°C-1and twofold, respectively, (all P < 0.05) with heating, but decreased or remained unchanged with cooling. In additional men, infusion of ATP into the brachial artery increased skin and deep tissue perfusion to levels equal or above thermal hyperaemia. In isolated erythrocyte samples exposed to different temperatures, ATP release increased 1.9-fold from 33 to 39°C (P < 0.05) and declined by ∼50% at 20°C (P < 0.05), but no changes were observed in cultured human endothelial cells, plasma or serum samples. In conclusion, increases in plasma [ATP] and skin and deep tissue perfusion with limb heating are associated with elevations in ATP release from erythrocytes, but not from endothelial cells or other blood constituents. Erythrocyte ATP release is also sensitive to temperature reductions, suggesting that erythrocytes may function as thermal sensors and ATP signalling generators for control of tissue perfusion during thermal interventions.
authors.affiliation: Centre for Human Performance, Exercise and Rehabilitation, Brunel University London, Uxbridge, UK.
authors.forename: Kameljit K
authors.identifier: 0000-0002-2386-7251
authors.initials: KK
authors.lastname: Kalsi
authors.affiliation: Centre for Human Performance, Exercise and Rehabilitation, Brunel University London, Uxbridge, UK.
authors.forename: Scott T
authors.initials: ST
authors.lastname: Chiesa
authors.affiliation: Centre for Human Performance, Exercise and Rehabilitation, Brunel University London, Uxbridge, UK.
authors.forename: Steven J
authors.initials: SJ
authors.lastname: Trangmar
authors.affiliation: Centre for Human Performance, Exercise and Rehabilitation, Brunel University London, Uxbridge, UK.|Department of Anaesthetics, Ealing Hospital NHS Trust, Southall, UK.
authors.forename: Leena
authors.initials: L
authors.lastname: Ali
authors.affiliation: Centre for Human Performance, Exercise and Rehabilitation, Brunel University London, Uxbridge, UK.|Department of Anaesthetics, Ealing Hospital NHS Trust, Southall, UK.
authors.forename: Makrand D
authors.initials: MD
authors.lastname: Lotlikar
authors.affiliation: Centre for Human Performance, Exercise and Rehabilitation, Brunel University London, Uxbridge, UK.
authors.forename: José
authors.identifier: 0000-0002-8205-3311
authors.initials: J
authors.lastname: González-Alonso
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism*; D000328:Adult; D001916:Brachial Artery / Q000378:metabolism; D042783:Endothelial Cells / Q000378:metabolism*; D004912:Erythrocytes / Q000378:metabolism*; D005121:Extremities / Q000098:blood supply / Q000502:physiology; D006801:Humans; D006940:Hyperemia / Q000378:metabolism; D008297:Male; D018482:Muscle, Skeletal / Q000098:blood supply / Q000378:metabolism; D012039:Regional Blood Flow / Q000502:physiology*; D012867:Skin / Q000098:blood supply* / Q000378:metabolism; D013696:Temperature; D055815:Young Adult
chemical_list: D000255:Adenosine Triphosphate