title: Silver Nanoparticles Impact Biofilm Communities and Mussel Settlement.
abstract: Silver nanoparticles (AgNPs) demonstrating good antimicrobial activity are widely used in many fields. However, the impact of AgNPs on the community structures of marine biofilms that drive biogeochemical cycling processes and the recruitment of marine invertebrate larvae remains unknown. Here, we employed MiSeq sequencing technology to evaluate the bacterial communities of 28-day-old marine biofilms formed on glass, polydimethylsiloxane (PDMS), and PDMS filled with AgNPs and subsequently tested the influence of these marine biofilms on plantigrade settlement by the mussel Mytilus coruscus. AgNP-filled PDMS significantly reduced the dry weight and bacterial density of biofilms compared with the glass and PDMS controls. AgNP incorporation impacted bacterial communities by reducing the relative abundance of Flavobacteriaceae (phylum: Bacteroidetes) and increasing the relative abundance of Vibrionaceae (phylum: Proteobacteria) in 28-day-old biofilms compared to PDMS. The settlement rate of M. coruscus on 28-day-old biofilms developed on AgNPs was lower by >30% compared to settlement on control biofilms. Thus, the incorporation of AgNPs influences biofilm bacterial communities in the marine environment and subsequently inhibits mussel settlement.
authors.affiliation: Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Shanghai Ocean University, Ministry of Education, China.|Marine Ecology Research Center, The First Institute of Oceanography, State Oceanic Administration, Qingdao, China.|Collaborative Innovation Center for Zhejiang Marine High-efficiency and Healthy Aquaculture, Ningbo, China.
authors.forename: Jin-Long
authors.initials: JL
authors.lastname: Yang
authors.affiliation: Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Shanghai Ocean University, Ministry of Education, China.
authors.forename: Yi-Feng
authors.initials: YF
authors.lastname: Li
authors.affiliation: Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Shanghai Ocean University, Ministry of Education, China.
authors.forename: Xiao
authors.initials: X
authors.lastname: Liang
authors.affiliation: Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Shanghai Ocean University, Ministry of Education, China.
authors.forename: Xing-Pan
authors.initials: XP
authors.lastname: Guo
authors.affiliation: Marine Ecology Research Center, The First Institute of Oceanography, State Oceanic Administration, Qingdao, China.
authors.forename: De-Wen
authors.initials: DW
authors.lastname: Ding
authors.affiliation: Collaborative Innovation Center for Zhejiang Marine High-efficiency and Healthy Aquaculture, Ningbo, China.
authors.forename: Demin
authors.initials: D
authors.lastname: Zhang
authors.affiliation: Department of Materials Science, State Key Laboratory of Molecular Engineering of Polymers, Advanced Coatings Research Center of Ministry of Education of China, Fudan University, Shanghai, China.
authors.forename: Shuxue
authors.initials: S
authors.lastname: Zhou
authors.affiliation: Institute of Marine Science and Technology, Yangzhou University, Yangzhou, China.
authors.forename: Wei-Yang
authors.initials: WY
authors.lastname: Bao
authors.affiliation: Hellenic Centre for Marine Research, Institute of Oceanography, Athens, Greece.
authors.forename: Nikoleta
authors.initials: N
authors.lastname: Bellou
authors.affiliation: Department of Marine Science and Fisheries, College of Agricultural and Marine Sciences, Sultan Qaboos University, Muscat, Oman.|Center of Excellence in Marine Biotechnology, Sultan Qaboos University, Muscat, Oman.
authors.forename: Sergey
authors.initials: S
authors.lastname: Dobretsov
mesh_terms: D000818:Animals; D018441:Biofilms / Q000187:drug effects*; D001681:Biological Assay; D018533:Biomass; D017377:Diatoms / Q000187:drug effects; D004129:Dimethylpolysiloxanes / Q000494:pharmacology; D053768:Metal Nanoparticles / Q000737:chemistry*; D049878:Mytilus / Q000187:drug effects*; D010802:Phylogeny; D025341:Principal Component Analysis; D012834:Silver / Q000494:pharmacology*
chemical_list: D004129:Dimethylpolysiloxanes; D012834:Silver; C013830:baysilon