title: Stable Discoidal Bicelles: A Platform of Lipid Nanocarriers for Cellular Delivery.
abstract: Bicellar mixtures have been used as alignable membrane substrates for the structural characterization of membrane-associated proteins. Most recently, it has been shown that bicelles can serve as nanocarriers to effectively deliver hydrophobic molecules to cancer cells with a 3- to 10-fold enhancement compared to that of chemically identical liposomes. In this chapter, a detailed preparation protocol, common structural characterization methods, the structural stability and the cellular uptake of bicellar nanodisks are discussed.
authors.affiliation: Department of Chemical and Biomolecular Engineering, University of Connecticut, Storrs, CT, 06269, USA.
authors.forename: Ying
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Department of Chemical and Biomolecular Engineering, University of Connecticut, Storrs, CT, 06269, USA.
authors.forename: Yan
authors.initials: Y
authors.lastname: Xia
authors.affiliation: Department of Biomedical Engineering, University of Connecticut, Storrs, CT, 06269, USA.
authors.forename: Armin Tahmasbi
authors.initials: AT
authors.lastname: Rad
authors.affiliation: Department of Biomedical Engineering, University of Connecticut, Storrs, CT, 06269, USA.
authors.forename: Wafa
authors.initials: W
authors.lastname: Aresh
authors.affiliation: Department of Chemical and Biomolecular Engineering, University of Connecticut, Storrs, CT, 06269, USA. mu-ping.nieh@uconn.edu.|Department of Biomedical Engineering, University of Connecticut, Storrs, CT, 06269, USA. mu-ping.nieh@uconn.edu.|Polymer Program, Institute of Materials Science, University of Connecticut, 97 North Eagleville Road, Unit 3136, Storrs, CT, 06269, USA. mu-ping.nieh@uconn.edu.
authors.forename: Mu-Ping
authors.initials: MP
authors.lastname: Nieh
mesh_terms: D000818:Animals; D002460:Cell Line; D004337:Drug Carriers / Q000737:chemistry*; D016503:Drug Delivery Systems; D000067493:Dynamic Light Scattering; D004705:Endocytosis; D006801:Humans; D008055:Lipids / Q000737:chemistry*; D008823:Micelles; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D033363:Neutron Diffraction; D053838:Scattering, Small Angle; D012997:Solvents
chemical_list: D004337:Drug Carriers; D008055:Lipids; D008823:Micelles; D012997:Solvents
keywords: Bicelles; Cellular uptake; Drug delivery carrier; Dynamic light scattering (DLS); PEGylated lipid; Phospholipids; Small angle X-ray scattering (SAXS); Small angle neutron scattering (SANS); Transmission electron microscopy (TEM)