title: A novel enzymatic approach based on the use of multi-enzymatic systems for the recovery of enriched protein extracts from potato pulp.
abstract: Ten commercial available multi-enzymatic systems have been explored for the efficient recovery of patatin and protease inhibitors from potato pulp. Their enzyme activity profile was characterized, where corresponding enzyme activity profile led to similar protein recovery yield. Of those assessed, Depol 670L (DEP) and Ceremix 2XL (CER) were efficient for the recovery of protein extract enriched with patatin (up to 60.0%) and protease inhibitors (up to 72.0%), respectively. The efficiency of DEP was significantly dependent on the interactive effect of enzymatic units and incubation time (p-value <0.015), while CER was found to be affected by the quadratric effect of both variables (p-value <0.022-0.052). The enriched patatin DEP-based protein extract possessed higher lipid acyl hydrolase activity emphasizing its preservation. The enriched protease inhibitors CER-based protein extract resulted in higher trypsin inhibiting activity, when compared to the industrial method. DEP and CER-based protein extracts were structurally characterized by peptide mass mapping.
authors.affiliation: Department of Food Science and Agricultural Chemistry, McGill University, 21 111 Lakeshore, Ste. Anne de Bellevue, Quebec H9X 3V9, Canada.
authors.forename: Amanda
authors.initials: A
authors.lastname: Waglay
authors.affiliation: Department of Food Science and Agricultural Chemistry, McGill University, 21 111 Lakeshore, Ste. Anne de Bellevue, Quebec H9X 3V9, Canada. Electronic address: salwa.karboune@mcgill.ca.
authors.forename: Salwa
authors.initials: S
authors.lastname: Karboune
mesh_terms: D002265:Carboxylic Ester Hydrolases / Q000032:analysis*; D008970:Molecular Weight; D010936:Plant Extracts / Q000032:analysis; D010940:Plant Proteins / Q000032:analysis*; D011480:Protease Inhibitors / Q000032:analysis*; D011198:Solanum tuberosum / Q000737:chemistry* / Q000201:enzymology*
chemical_list: D010936:Plant Extracts; D010940:Plant Proteins; D011480:Protease Inhibitors; C043222:patatin protein, Solanum tuberosum; D002265:Carboxylic Ester Hydrolases
keywords: Multi-enzymatic systems; Optimization; Potato pulp; Protein recovery