title: Phytochemical Content and Pharma-Nutrition Study onEleutherococcus senticosusFruits Intractum.
abstract: In the past two decades public interest in herbal products has increased significantly in Europe, especially in the plant-based products from non-European traditions.Eleutherococcus senticosushas been used for the treatment of inflammatory diseases, anemia, and rheumatoid arthritis. TheEleutherococcus senticosusfruits intractum was examined for the content of phenolic acids (LC-ESI-MS/MS), minerals (AAS), TPC, and TFC (spectrophotometric assay). The antioxidant activity was determined using free radical scavenging assay and TLC-DB-DPPH∗dot-blot test. An anti-Hyal activity was evaluated by the spectrophotometric assay method. Cytotoxicity towards HL-60, HL-60/MX1, HL-60/MX2, CEM/C1, and CCRF/CEM leukemic cell lines was done using trypan blue test. Among eight phenolic acids,trans-caffeic acid was found in the largest amount (41.2 mg/g DE). The intractum presented a high amount of macroelements (Ca, Mg, K; 1750, 1300, and 21000 mg/kg) and microelements (Fe, Mn; 32.7, 54.3 mg/kg), respectively. The content of TPC and TFC was 130 and 92 mg/g DE, respectively. The intractum showed anti-Hyal activity (2.16-60%) and an antioxidant capacity (EC50; 52μg/mL). The intractum most strongly inhibited the growth of HL-60, HL-60/MX1, and CCRF/CEM. A better understanding of the intractum health benefits is important in order to increase its utility and enrich dietary sources of health promoting compounds.
authors.affiliation: Department of Pharmacognosy, Ludwik Rydygier Collegium Medicum, Nicolaus Copernicus University, 9 Marie Curie-Skłodowska Street, 85-094 Bydgoszcz, Poland.
authors.forename: Daniel
authors.identifier: 0000-0002-7821-7002
authors.initials: D
authors.lastname: Załuski
authors.affiliation: Department of Pharmaceutical Botany, Medical University of Lublin, 1 Chodźki Street, 20-093 Lublin, Poland.
authors.forename: Marta
authors.initials: M
authors.lastname: Olech
authors.affiliation: Department of Pharmacognosy, Collegium Medicum, Jagiellonian University, 9 Medyczna Street, 30-688 Cracow, Poland.
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Galanty
authors.affiliation: Natural Products Laboratory, Institute of Biology, Leiden University, 2300 RA Leiden, Netherlands.
authors.forename: Robert
authors.initials: R
authors.lastname: Verpoorte
authors.affiliation: Department of Pharmacognosy, Ludwik Rydygier Collegium Medicum, Nicolaus Copernicus University, 9 Marie Curie-Skłodowska Street, 85-094 Bydgoszcz, Poland.
authors.forename: Rafał
authors.identifier: 0000-0002-8250-707X
authors.initials: R
authors.lastname: Kuźniewski
authors.affiliation: Department of Pharmaceutical Botany, Medical University of Lublin, 1 Chodźki Street, 20-093 Lublin, Poland.
authors.forename: Renata
authors.initials: R
authors.lastname: Nowak
authors.affiliation: Department of Biology and Genetics, Medical University of Lublin, 4a Chodźki Street, 20-093 Lublin, Poland.
authors.forename: Anna
authors.initials: A
authors.lastname: Bogucka-Kocka
mesh_terms: D000975:Antioxidants / Q000737:chemistry; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002851:Chromatography, High Pressure Liquid; D028001:Eleutherococcus / Q000737:chemistry* / Q000378:metabolism; D005638:Fruit / Q000737:chemistry / Q000378:metabolism; D006801:Humans; D006821:Hyaluronoglucosaminidase / Q000037:antagonists & inhibitors / Q000378:metabolism; D064209:Phytochemicals / Q000032:analysis / Q000737:chemistry* / Q000633:toxicity; D010936:Plant Extracts / Q000737:chemistry*; D013329:Structure-Activity Relationship; D053719:Tandem Mass Spectrometry
chemical_list: D000975:Antioxidants; D064209:Phytochemicals; D010936:Plant Extracts; D006821:Hyaluronoglucosaminidase