title: Production and characterization of polyhydroxybutyrate from Vibrio harveyi MCCB 284 utilizing glycerol as carbon source.
abstract: OBJECTIVE
  Production and characterization of polyhydroxybutyrate (PHB) from moderately halophilic bacterium Vibrio harveyi MCCB 284 isolated from tunicate Phallusia nigra.
  
  
  RESULTS
  Twenty-five bacterial isolates were obtained from tunicate samples and three among them exhibited an orange fluorescence in Nile red staining indicating the presence of PHB. One of the isolates, MCCB 284, which showed rapid growth and good polymer yield, was identified as V. harveyi. The optimum conditions of the isolate for the PHB production were pH 8·0, sodium chloride concentration 20 g l-1, inoculum size 0·5% (v/v), glycerol 20 g l-1and 72 h of incubation at 30°C. Cell dry weight (CDW) of 3·2 g l-1, PHB content of 2·3 g l-1and final PHB yield of 1·2 g l-1were achieved. The extracted PHB was characterized by FTIR, NMR and DSC-TGA techniques.
  
  
  CONCLUSIONS
  An isolate of V. harveyi that could effectively utilize glycerol for growth and PHB accumulation was obtained from tunicate P. nigra. PHB produced was up to 72% based on CDW.
  
  
  CONCLUSIONS
  This is the first report of an isolate of V. harveyi which utilizes glycerol as the sole carbon source for PHB production with high biomass yield. This isolate could be of use as candidate species for commercial PHB production using glycerol as the feed stock or as source of genes for recombinant PHB production or for synthetic biology.
authors.affiliation: National Centre for Aquatic Animal Health, Cochin University of Science and Technology, Kochi, Kerala, India.
authors.forename: S P
authors.initials: SP
authors.lastname: Mohandas
authors.affiliation: National Centre for Aquatic Animal Health, Cochin University of Science and Technology, Kochi, Kerala, India.
authors.forename: L
authors.identifier: http://orcid.org/0000-0002-4575-408X
authors.initials: L
authors.lastname: Balan
authors.affiliation: National Centre for Aquatic Animal Health, Cochin University of Science and Technology, Kochi, Kerala, India.
authors.forename: N
authors.initials: N
authors.lastname: Lekshmi
authors.affiliation: Centre for Marine Living Resources and Ecology, Kakkanad, Kochi, Kerala, India.
authors.forename: S S
authors.initials: SS
authors.lastname: Cubelio
authors.affiliation: Department of Marine Biology, Microbiology and Biochemistry, Cochin University of Science and Technology, Kochi, Kerala, India.
authors.forename: R
authors.initials: R
authors.lastname: Philip
authors.affiliation: National Centre for Aquatic Animal Health, Cochin University of Science and Technology, Kochi, Kerala, India.
authors.forename: I S
authors.initials: IS
authors.lastname: Bright Singh
mesh_terms: D018533:Biomass; D002244:Carbon / Q000378:metabolism*; D005990:Glycerol / Q000378:metabolism*; D006885:Hydroxybutyrates / Q000737:chemistry / Q000378:metabolism*; D011108:Polymers; D014733:Vibrio / Q000378:metabolism*
chemical_list: D006885:Hydroxybutyrates; D011108:Polymers; D002244:Carbon; D005990:Glycerol
keywords: 
  Vibrio harveyi
  ; characterization of PHB; glycerol; marine bacteria; polyhydroxybutyrate