title: Additive effect ofMTHFRandGRIN1genetic polymorphisms on the risk of schizophrenia.
abstract: Schizophrenia is a complex disorder with polygenic inheritance. TheMTHFRgene (OMIM: 607093) plays an important role in the folate metabolism. It has been suggested that C677T (rs1801133) and A1298C (rs1801131) genetic polymorphisms in theMTHFRgene lead to the decreased activity of the methylenetetrahydrofolate reductase enzyme which may have significant effect on developing schizophrenia. We used a case-control study to establish the possible association between the C677T and the A1298C polymorphisms and susceptibility to schizophrenia in an Iranian population. The genotypes of the polymorphisms were determined using PCR-RFLP. The data were analyzed by logistic regression model. Data analysis revealed that the combination genotypes of 677CT/1298AA, 677CC/1298CC, 677TT/1298AA, 677CT/1298AC and 677CT/1298CC increase the risk of schizophrenia. In order to evaluate the effect of combined genotypes of the three mentioned polymorphic loci, the frequencies of the compound genotypes were compared between control and patient groups (Table 4). Base on the results, the existence of >4 risk factors showed about 32-fold increased risk for schizophrenia (OR=32.3, 95% CI: 5.52-188, P=<0.001).
authors.affiliation: Department of Genetics, College of Sciences, Shahid Chamran University, Ahvaz 61357-44337, Iran.
authors.forename: Ali Mohammad
authors.initials: AM
authors.lastname: Foroughmand
authors.affiliation: Department of Genetics, College of Sciences, Shahid Chamran University, Ahvaz 61357-44337, Iran.
authors.forename: Hamid
authors.initials: H
authors.lastname: Galehdari
authors.affiliation: Department of Genetics, College of Sciences, Shahid Chamran University, Ahvaz 61357-44337, Iran.
authors.forename: Atefeh
authors.initials: A
authors.lastname: Pooryasin
authors.affiliation: Department of Genetics, College of Sciences, Shahid Chamran University, Ahvaz 61357-44337, Iran.
authors.forename: Tahereh
authors.initials: T
authors.lastname: Ajam
authors.affiliation: Department of Genetics, College of Sciences, Shahid Chamran University, Ahvaz 61357-44337, Iran.
authors.forename: Seyed Reza
authors.initials: SR
authors.lastname: Kazemi-Nezhad
keywords: GRIN1; Homocysteine; MTHFR; NMDA; Schizophrenia