title: A functional endosomal pathway is necessary for lysosome biogenesis in Drosophila.
abstract: BACKGROUND
  Lysosomes are the major catabolic compartment within eukaryotic cells, and their biogenesis requires the integration of the biosynthetic and endosomal pathways. Endocytosis and autophagy are the primary inputs of the lysosomal degradation pathway. Endocytosis is specifically needed for the degradation of membrane proteins whereas autophagy is responsible for the degradation of cytoplasmic components. We previously identified the deubiquitinating enzyme UBPY/USP8 as being necessary for lysosomal biogenesis and productive autophagy in Drosophila. Because UBPY/USP8 has been widely described for its function in the endosomal system, we hypothesized that disrupting the endosomal pathway itself may affect the biogenesis of the lysosomes.
  
  
  RESULTS
  In the present study, we blocked the progression of the endosomal pathway at different levels of maturation of the endosomes by expressing in fat body cells either dsRNAs or dominant negative mutants targeting components of the endosomal machinery: Shibire, Rab4, Rab5, Chmp1 and Rab7. We observed that inhibition of endosomal trafficking at different steps in vivo is systematically associated with defects in lysosome biogenesis, resulting in autophagy flux blockade.
  
  
  CONCLUSIONS
  Our results show that the integrity of the endosomal system is required for lysosome biogenesis and productive autophagy in vivo.
authors.affiliation: Université Grenoble-Alpes, F-38041, Grenoble, France.|CEA-DSV-iRTSV-BGE-Gen&Chem, 17, rue des Martyrs, 38054, Grenoble, Cedex 9, France.|INSERM, U1038, F-38054, Grenoble, France.|Present address: School of Life Sciences, University of Warwick, Coventry, UK.
authors.forename: Anne-Claire
authors.initials: AC
authors.lastname: Jacomin
authors.affiliation: Université Grenoble-Alpes, F-38041, Grenoble, France. marie-odile.fauvarque@cea.fr.|CEA-DSV-iRTSV-BGE-Gen&Chem, 17, rue des Martyrs, 38054, Grenoble, Cedex 9, France. marie-odile.fauvarque@cea.fr.|INSERM, U1038, F-38054, Grenoble, France. marie-odile.fauvarque@cea.fr.
authors.forename: Marie-Odile
authors.initials: MO
authors.lastname: Fauvarque
authors.affiliation: Université Grenoble-Alpes, F-38041, Grenoble, France. emmanuel.taillebourg@cea.fr.|CEA-DSV-iRTSV-BGE-Gen&Chem, 17, rue des Martyrs, 38054, Grenoble, Cedex 9, France. emmanuel.taillebourg@cea.fr.|INSERM, U1038, F-38054, Grenoble, France. emmanuel.taillebourg@cea.fr.
authors.forename: Emmanuel
authors.initials: E
authors.lastname: Taillebourg
mesh_terms: D000818:Animals; D000071182:Autophagosomes / Q000378:metabolism; D001343:Autophagy; D056668:Cathepsin L / Q000378:metabolism; D004331:Drosophila melanogaster / Q000378:metabolism*; D011992:Endosomes / Q000378:metabolism*; D052118:Lysosomal-Associated Membrane Protein 1 / Q000378:metabolism; D008247:Lysosomes / Q000378:metabolism*
chemical_list: D052118:Lysosomal-Associated Membrane Protein 1; D056668:Cathepsin L
keywords: Autophagy; Drosophila melanogaster; Endocytosis; Endosomal system; Lysosomal biogenesis; Lysosome