title: The Lnc RNA SPRY4-IT1 Modulates Trophoblast Cell Invasion and Migration by Affecting the Epithelial-Mesenchymal Transition.
abstract: Preeclampsia is a common, pregnancy-specific disease and a major contributor to maternal and foetal morbidity and mortality. Some placental abnormalities, including deficient implantation, abnormal trophoblast cell function, and improper placental vascular development, are believed to lead to preeclampsia. The long noncoding RNA SPRY4-IT1 is more highly expressed in preeclamptic human placentas than in normal placentas. We assessed the role of epithelial-mesenchymal transition (EMT)-associated invasion and migration in HTR-8/SVneo trophoblast cells. Overexpression of SPRY4-IT1 suppressed trophoblast cell migration and invasion, whereas reduced expression of SPRY4-IT1 prevented the EMT process. Mechanistically, an RNA immunoprecipitation experiment showed that SPRY4-IT1 bound directly to HuR and mediated the β-catenin expression associated with EMT in HTR-8/SVneo cells. Moreover, the expression levels of genes in the WNT family, such as WNT3 and WNT5B, were changed after transfection of HTR-8/SVneo with SPRY4-IT1. Together, our results highlight the roles of SPRY4-IT1 in causing trophoblast cell dysfunction by acting through the Wnt/β-catenin pathway, and consequently in impairing spiral artery remodelling. These results suggest a new potential therapeutic target for intervention against preeclampsia.
authors.affiliation: Department of Obstetrics and Gynecology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, JiangSu Province, 210029, China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Zuo
authors.affiliation: Department of Obstetrics and Gynecology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, JiangSu Province, 210029, China.
authors.forename: Shiyun
authors.initials: S
authors.lastname: Huang
authors.affiliation: Departments of Obstetrics and Gynecology, Yuhuangding Hospital of Yantai, Yantai, Shandong Province, 264000, China.
authors.forename: Yanfen
authors.initials: Y
authors.lastname: Zou
authors.affiliation: Department of Obstetrics and Gynecology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, JiangSu Province, 210029, China.
authors.forename: Yetao
authors.initials: Y
authors.lastname: Xu
authors.affiliation: Department of Obstetrics and Gynecology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, JiangSu Province, 210029, China.
authors.forename: Ziyan
authors.initials: Z
authors.lastname: Jiang
authors.affiliation: Department of Obstetrics and Gynecology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, JiangSu Province, 210029, China.
authors.forename: Shan
authors.initials: S
authors.lastname: Zou
authors.affiliation: The Family Planning Science and Technology Research Institute of Jiangsu province, Nanjing, JiangSu Province, 210029, China.
authors.forename: Haoqing
authors.initials: H
authors.lastname: Xu
authors.affiliation: Department of Obstetrics and Gynecology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, JiangSu Province, 210029, China.
authors.forename: Lizhou
authors.initials: L
authors.lastname: Sun
mesh_terms: D002460:Cell Line; D002465:Cell Movement; D000067780:ELAV-Like Protein 1 / Q000235:genetics / Q000378:metabolism; D058750:Epithelial-Mesenchymal Transition; D005260:Female; D006801:Humans; D011225:Pre-Eclampsia / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D011247:Pregnancy; D062085:RNA, Long Noncoding / Q000235:genetics / Q000378:metabolism*; D014327:Trophoblasts / Q000378:metabolism* / Q000473:pathology; D060449:Wnt Signaling Pathway; D051176:beta Catenin / Q000235:genetics / Q000378:metabolism
chemical_list: D000067780:ELAV-Like Protein 1; C098770:ELAVL1 protein, human; D062085:RNA, Long Noncoding; D051176:beta Catenin; C000605823:long noncoding RNA SPRY4-IT1, human