title: Olive oil abrogates acrylamide induced nephrotoxicity by modulating biochemical and histological changes in rats.
abstract: Acrylamide (ACR) is one of the most important contaminants occurring in foods heated at high temperatures. The aim of this study is to investigate the protective efficacy of extra virgin olive oil (EVOO), a main component of the Mediterranean diet, against nephrotoxicity induced by ACR. Rats have received by gavage during 21 days either ACR (40 mg/kg body weight) or ACR-associated with EVOO (300 μl) or only EVOO (300 μl). Acrylamide induced nephrotoxicity as evidenced by an increase in malondialdehyde (MDA), hydrogen peroxide (H2O2), protein carbonyls (PCOs) and a decrease in glutathione, non-protein thiols (NPSHs), and vitamin C levels. Activities of catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GPx) were also decreased. Lactate dehydrogenase (LDH) activity, creatinine, urea, and uric acid, urinary volume and creatinine clearance levels were modified. EVOO supplementation improved all the parameters indicated above. Kidney histoarchitecture confirmed the biochemical parameters and the beneficial role of EVOO. EVOO, when added to the diet, may have a beneficial role against kidney injury by scavenging free radicals and by its potent antioxidant power.
authors.affiliation: a Animal Physiology Laboratory, Faculty of Sciences of Sfax , University of Sfax , Sfax , Tunisia.
authors.forename: Imen
authors.initials: I
authors.lastname: Ghorbel
authors.affiliation: a Animal Physiology Laboratory, Faculty of Sciences of Sfax , University of Sfax , Sfax , Tunisia.
authors.forename: Awatef
authors.initials: A
authors.lastname: Elwej
authors.affiliation: b Biochemistry Laboratory, CHU Hedi Chaker , University of Sfax , Sfax , Tunisia.
authors.forename: Nesrine
authors.initials: N
authors.lastname: Fendri
authors.affiliation: c Anatomopathology Laboratory, CHU Habib Bourguiba , University of Sfax , Sfax , Tunisia.
authors.forename: Héla
authors.initials: H
authors.lastname: Mnif
authors.affiliation: b Biochemistry Laboratory, CHU Hedi Chaker , University of Sfax , Sfax , Tunisia.
authors.forename: Kamel
authors.initials: K
authors.lastname: Jamoussi
authors.affiliation: c Anatomopathology Laboratory, CHU Habib Bourguiba , University of Sfax , Sfax , Tunisia.
authors.forename: Tahia
authors.initials: T
authors.lastname: Boudawara
authors.affiliation: d Technology & Quality Research Unit , Olive Tree Institute, University of Sfax , Sfax , Tunisia.
authors.forename: Naziha
authors.initials: N
authors.lastname: Grati Kamoun
authors.affiliation: a Animal Physiology Laboratory, Faculty of Sciences of Sfax , University of Sfax , Sfax , Tunisia.
authors.forename: Najiba
authors.initials: N
authors.lastname: Zeghal
mesh_terms: D020106:Acrylamide / Q000633:toxicity*; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D002374:Catalase / Q000378:metabolism; D019587:Dietary Supplements; D005260:Female; D005978:Glutathione / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000378:metabolism; D007668:Kidney / Q000503:physiopathology; D007674:Kidney Diseases / Q000517:prevention & control*; D007770:L-Lactate Dehydrogenase / Q000097:blood; D008315:Malondialdehyde / Q000378:metabolism; D000069463:Olive Oil / Q000494:pharmacology*; D018384:Oxidative Stress / Q000187:drug effects*; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D000975:Antioxidants; D000069463:Olive Oil; D020106:Acrylamide; D008315:Malondialdehyde; D006861:Hydrogen Peroxide; D007770:L-Lactate Dehydrogenase; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D005978:Glutathione
keywords: Acrylamide; antioxidant status; extra virgin olive oil; nephrotoxicity; rats