title: Assembly and analysis of the completeSalix purpureaL. (Salicaceae) mitochondrial genome sequence.
abstract: Plant mitochondrial (mt) genomes possess several complex features, including a variable size, a dynamic genome structure, and complicated patterns of gene loss and gain throughout evolutionary history. Studies of plant mt genomes can, therefore, provide unique insights into organelle evolution. We assembled the completeSalix purpureaL. mt genome by screening genomic sequence reads generated by a Roche-454 pyrosequencing platform. The pseudo-molecule obtained has a typical circular structure 598,970 bp long, with an overall GC content of 55.06%. TheS. purpureamt genome contains 52 genes: 31 protein-coding, 18 tRNAs, and three rRNAs. Eighteen tandem repeats and 404 microsatellites are distributed unevenly throughout theS. purpureamt genome. A phylogenetic tree of 23 representative terrestrial plants strongly supportsS. purpureainclusion in the Malpighiales clade. Our analysis contributes toward understanding the organization and evolution of organelle genomes in Salicaceae species.
authors.affiliation: College of Forestry, Nanjing Forestry University, Nanjing, 210037 Jiangsu China ; The Southern Modern Forestry Collaborative Innovation Center, Nanjing Forestry University, Nanjing, 210037 Jiangsu China ; College of Information Science and Technology, Nanjing Forestry University, Nanjing, 210037 Jiangsu China.
authors.forename: Suyun
authors.initials: S
authors.lastname: Wei
authors.affiliation: College of Information Science and Technology, Nanjing Forestry University, Nanjing, 210037 Jiangsu China.
authors.forename: Xuelin
authors.initials: X
authors.lastname: Wang
authors.affiliation: College of Information Science and Technology, Nanjing Forestry University, Nanjing, 210037 Jiangsu China.
authors.forename: Changwei
authors.initials: C
authors.lastname: Bi
authors.affiliation: College of Information Science and Technology, Nanjing Forestry University, Nanjing, 210037 Jiangsu China ; School of Computer Science and Engineering, Southeast University, Nanjing, 211189 Jiangsu China.
authors.forename: Yiqing
authors.initials: Y
authors.lastname: Xu
authors.affiliation: College of Forestry, Nanjing Forestry University, Nanjing, 210037 Jiangsu China ; The Southern Modern Forestry Collaborative Innovation Center, Nanjing Forestry University, Nanjing, 210037 Jiangsu China ; College of Information Science and Technology, Nanjing Forestry University, Nanjing, 210037 Jiangsu China.
authors.forename: Dongyang
authors.initials: D
authors.lastname: Wu
authors.affiliation: The Southern Modern Forestry Collaborative Innovation Center, Nanjing Forestry University, Nanjing, 210037 Jiangsu China ; College of Information Science and Technology, Nanjing Forestry University, Nanjing, 210037 Jiangsu China.
authors.forename: Ning
authors.identifier: 0000-0001-7249-8352
authors.initials: N
authors.lastname: Ye
keywords: Genome assembly; Mitochondrial genome; Phylogenetic tree; Salicaceae