title: IL-2 complex treatment amplifies CD8+T cell mediated immunity following herpes simplex virus-1 infection.
abstract: CD8+T cells play an important role in controlling numerous virus infections and some tumors and therefore several strategies have been adopted to modulate CD8+T cell responses. One such approach includes treatment with IL-2 bound to a monoclonal antibody against IL-2 (IL-2 complex) which was shown to enhance CD8+T cell responses and provide protection against some cancers and pathogens. This report analyses the value of IL-2 complex therapy to protect against a cutaneous virus infection as occurs with herpes simplex virus-1 (HSV-1) infection. Treatment with IL-2 complex after infection reduced virus levels and lesion severity in a zosteriform model of HSV infection in mice. Furthermore, IL-2 complex treatment expanded HSV-1-gB epitope-specific CD8+T cells, IFN-γ and TNF-α producing CD8+T cells as well as cells that produced more than one cytokine. In addition, IL-2 complex therapy recipients showed enhanced cytolytic activity of CD8+T cells as shown by increased granzyme B expression and lytic granule release. Taken, together, these studies demonstrate that IL-2 complex therapy can be useful to boost protection against a cutaneous virus infection.
authors.affiliation: Biomedical & Diagnostic Sciences, College of Veterinary Medicine, The University of Tennessee, Knoxville, TN, 37996-0845, United States.
authors.forename: Naveen K
authors.initials: NK
authors.lastname: Rajasagi
authors.affiliation: Biomedical & Diagnostic Sciences, College of Veterinary Medicine, The University of Tennessee, Knoxville, TN, 37996-0845, United States. Electronic address: btr@utk.edu.
authors.forename: Barry T
authors.initials: BT
authors.lastname: Rouse
mesh_terms: D000818:Animals; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D004195:Disease Models, Animal; D005260:Female; D006561:Herpes Simplex / Q000276:immunology* / Q000473:pathology / Q000628:therapy*; D018259:Herpesvirus 1, Human / Q000276:immunology*; D007111:Immunity, Cellular; D018796:Immunoconjugates / Q000627:therapeutic use*; D007376:Interleukin-2 / Q000627:therapeutic use*; D008810:Mice, Inbred C57BL; D016896:Treatment Outcome
chemical_list: D018796:Immunoconjugates; D007376:Interleukin-2
keywords: CD8 T cells; Herpes simplex virus-1; IL-2 complex