title: Porcine Pancreatic Lipase Inhibitory Agent Isolated from Medicinal Herb and Inhibition Kinetics of Extracts fromEleusine indica(L.) Gaertner.
abstract: Eleusine indica(Linnaeus) Gaertner is a traditional herb known to be depurative, febrifuge, and diuretic and has been reported with the highest inhibitory activity against porcine pancreatic lipase (PPL) among thirty two plants screened in an earlier study. This study aims to isolate and identify the active components that may possess high potential as an antiobesity agent. Of the screened solvent fractions ofE. indica, hexane fraction showed the highest inhibitory activity of 27.01 ± 5.68% at 100μg/mL. Bioactivity-guided isolation afforded three compounds from the hexane fraction ofE. indica, namely,β-sitosterol, stigmasterol, and lutein. The structures of these compounds were elucidated using spectral techniques. Lutein showed an outstanding inhibitory activity against PPL (55.98 ± 1.04%), with activity 60% higher than that of the reference drug Orlistat. The other compounds isolated and identified wereβ-sitosterol (2.99 ± 0.80%) and stigmasterol (2.68 ± 0.38%). The enzyme kinetics ofE. indicacrude methanolic extract on PPL showed mixed inhibition mechanism.
authors.affiliation: School of Biosciences, Taylor's University, No. 1 Jalan Taylor's, 47500 Subang Jaya, Malaysia.
authors.forename: Siew Ling
authors.initials: SL
authors.lastname: Ong
authors.affiliation: School of Biosciences, Taylor's University, No. 1 Jalan Taylor's, 47500 Subang Jaya, Malaysia.
authors.forename: Siau Hui
authors.identifier: 0000-0003-0370-4866
authors.initials: SH
authors.lastname: Mah
authors.affiliation: School of Biosciences, Taylor's University, No. 1 Jalan Taylor's, 47500 Subang Jaya, Malaysia.
authors.forename: How Yee
authors.identifier: 0000-0003-3615-336X
authors.initials: HY
authors.lastname: Lai