title: Immunoassay and molecular methods to investigate DNA methylation changes in peripheral blood mononuclear cells in HIV infected patients on cART.
abstract: This study aimed to investigate the influence of antiretroviral therapy on methylation markers, in a group of HIV infected, heavily treated patients. Immune and molecular methods were used to investigate potential changes in methylation profile in DNA isolated from peripheral blood mononuclear cells collected from antiretroviral-experienced HIV infected patients and healthy controls. The percentage of 5-methylcytosine was inversely correlated with proviral DNA and active replication while DNMT1 (p = 0.01) and DNMT3A (p = 0.004) independently correlated with active viral replication. DNMT3A expression increased with total treatment duration (p = 0.03), number of antiretroviral drugs ever used (p = 0.003), and cumulative exposure to protease inhibitors (p = 0.02) even in currently HIV undetectable patients.
authors.affiliation: a Department of Virology , Carol Davila University of Medicine and Pharmacy , Bucharest , Romania.|b Emerging Viral Diseases Department , Stefan S. Nicolau Virology Institute , Bucharest , Romania.
authors.forename: Adelina
authors.initials: A
authors.lastname: Rosca
authors.affiliation: b Emerging Viral Diseases Department , Stefan S. Nicolau Virology Institute , Bucharest , Romania.
authors.forename: Gabriela
authors.initials: G
authors.lastname: Anton
authors.affiliation: c Infectious Diseases Department , Victor Babes Hospital for Infectious and Tropical Diseases , Bucharest , Romania.
authors.forename: Luminita
authors.initials: L
authors.lastname: Ene
authors.affiliation: b Emerging Viral Diseases Department , Stefan S. Nicolau Virology Institute , Bucharest , Romania.
authors.forename: Iulia
authors.initials: I
authors.lastname: Iancu
authors.affiliation: a Department of Virology , Carol Davila University of Medicine and Pharmacy , Bucharest , Romania.|b Emerging Viral Diseases Department , Stefan S. Nicolau Virology Institute , Bucharest , Romania.
authors.forename: Aura
authors.initials: A
authors.lastname: Temereanca
authors.affiliation: d Department of Psychiatry , University of California , San Diego , California.
authors.forename: Cristian L
authors.initials: CL
authors.lastname: Achim
authors.affiliation: a Department of Virology , Carol Davila University of Medicine and Pharmacy , Bucharest , Romania.|b Emerging Viral Diseases Department , Stefan S. Nicolau Virology Institute , Bucharest , Romania.
authors.forename: Simona M
authors.initials: SM
authors.lastname: Ruta
mesh_terms: D000328:Adult; D019380:Anti-HIV Agents / Q000627:therapeutic use*; D023241:Antiretroviral Therapy, Highly Active; D019175:DNA Methylation / Q000276:immunology*; D005260:Female; D015658:HIV Infections / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology*; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D007963:Leukocytes, Mononuclear / Q000378:metabolism* / Q000473:pathology / Q000821:virology; D008297:Male; D055815:Young Adult
chemical_list: D019380:Anti-HIV Agents
keywords: DNA methylation; DNMT1; DNMT3A; HIV; PBMC; antiretroviral treatment; epigenetics