title: Fluorescence-based lateral flow assays for rapid oral fluid roadside detection of cannabis use.
abstract: With the recent worldwide changes in the legalization of marijuana, there is a significant need for rapid, roadside screening test for driving under the influence of drugs. A robust, sensitive, lateral flow assay has been developed to detect recent use via oral-fluid testing for Δ9-tetrahydrocannabinol (THC). This proof-of-concept assay uses a fluorescent-based immunoassay detection of polymeric beads, conjugated to antibodies against native THC. The fluorescent technique allows for significantly lower limits of detection and higher precision determination of recent marijuana use without the use of urine or blood sampling-thus allowing for roadside identification. Detection levels of 0.01 ng/mL were distinguished from background and the lower limit of quantification was determined to approach 1 ng/mL.
authors.affiliation: Department of STEM, Regis College, Weston, MA, USA.|Department of Chemical Engineering and Barnett Institute of Chemical and Biological Analysis, Northeastern University, Boston, MA, USA.
authors.forename: Brian D
authors.initials: BD
authors.lastname: Plouffe
authors.affiliation: Department of Chemical Engineering and Barnett Institute of Chemical and Biological Analysis, Northeastern University, Boston, MA, USA.
authors.forename: Shashi K
authors.initials: SK
authors.lastname: Murthy
mesh_terms: D001334:Automobile Driving; D002188:Cannabis; D013759:Dronabinol / Q000032:analysis*; D015200:Fluoroimmunoassay / Q000379:methods*; D006801:Humans; D057230:Limit of Detection; D016014:Linear Models; D008385:Marijuana Smoking; D046210:Microfluidic Analytical Techniques / Q000379:methods; D015203:Reproducibility of Results; D012463:Saliva / Q000737:chemistry*; D015813:Substance Abuse Detection / Q000379:methods*
chemical_list: D013759:Dronabinol
keywords: Fluorescence; Lateral flow assay; Marijuana; Roadside detection; THC