title: Insight into the metabolic mechanism of scoparone on biomarkers for inhibiting Yanghuang syndrome.
abstract: Scoparone (6,7-dimethoxycoumarin) is the representative ingredient of Yinchenhao (Artemisia capillaris Thunb.) which is a famous Chinese medicinal herb and shows favorable efficacy for all kinds of liver disease, specifically for the treatment of Yanghuang syndrome (YHS). The precise molecular mechanism concerning the action of scoparone on YHS is yet to be fully elucidated. The aim of the present study was to determine the mechanism of scoparone and evaluate its efficacy on metabolite levels. The differential expression of metabolites responsible for the pharmacological effects of scoparone was characterized and the protection effect of scoparone against this disease. Using multivariate statistical analysis, 33 biomarkers were identified using precise MS/MS and play an important role in the regulation of key metabolic pathways associated with liver disease. In addition, pathological results also showed consistent changes in the YHS model group and after treatment with scoparone, both the metabolic profile and histopathology resembled that of normal level, which suggesting favorable efficacy over the observed time period. The present work indicated that a metabolomics platform provided a new insight into understanding the mechanisms of action of natural medicines such as scoparone.
authors.affiliation: Sino-America Chinmedomics Technology Cooperation Center, Chinmedomics Research Center of TCM State Administration, National TCM Key Laboratory of Serum Pharmacochemistry, Department of Pharmaceutical Analysis, Laboratory of Metabolomics, Heilongjiang University of Chinese Medicine, Heping Road 24, Harbin 150040, China.
authors.forename: Heng
authors.initials: H
authors.lastname: Fang
authors.affiliation: Sino-America Chinmedomics Technology Cooperation Center, Chinmedomics Research Center of TCM State Administration, National TCM Key Laboratory of Serum Pharmacochemistry, Department of Pharmaceutical Analysis, Laboratory of Metabolomics, Heilongjiang University of Chinese Medicine, Heping Road 24, Harbin 150040, China.
authors.forename: Aihua
authors.initials: A
authors.lastname: Zhang
authors.affiliation: Sino-America Chinmedomics Technology Cooperation Center, Chinmedomics Research Center of TCM State Administration, National TCM Key Laboratory of Serum Pharmacochemistry, Department of Pharmaceutical Analysis, Laboratory of Metabolomics, Heilongjiang University of Chinese Medicine, Heping Road 24, Harbin 150040, China.
authors.forename: Jingbo
authors.initials: J
authors.lastname: Yu
authors.affiliation: Sino-America Chinmedomics Technology Cooperation Center, Chinmedomics Research Center of TCM State Administration, National TCM Key Laboratory of Serum Pharmacochemistry, Department of Pharmaceutical Analysis, Laboratory of Metabolomics, Heilongjiang University of Chinese Medicine, Heping Road 24, Harbin 150040, China.
authors.forename: Liang
authors.initials: L
authors.lastname: Wang
authors.affiliation: Sino-America Chinmedomics Technology Cooperation Center, Chinmedomics Research Center of TCM State Administration, National TCM Key Laboratory of Serum Pharmacochemistry, Department of Pharmaceutical Analysis, Laboratory of Metabolomics, Heilongjiang University of Chinese Medicine, Heping Road 24, Harbin 150040, China.
authors.forename: Chang
authors.initials: C
authors.lastname: Liu
authors.affiliation: Sino-America Chinmedomics Technology Cooperation Center, Chinmedomics Research Center of TCM State Administration, National TCM Key Laboratory of Serum Pharmacochemistry, Department of Pharmaceutical Analysis, Laboratory of Metabolomics, Heilongjiang University of Chinese Medicine, Heping Road 24, Harbin 150040, China.
authors.forename: Xiaohang
authors.initials: X
authors.lastname: Zhou
authors.affiliation: Sino-America Chinmedomics Technology Cooperation Center, Chinmedomics Research Center of TCM State Administration, National TCM Key Laboratory of Serum Pharmacochemistry, Department of Pharmaceutical Analysis, Laboratory of Metabolomics, Heilongjiang University of Chinese Medicine, Heping Road 24, Harbin 150040, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Sun
authors.affiliation: Sino-America Chinmedomics Technology Cooperation Center, Chinmedomics Research Center of TCM State Administration, National TCM Key Laboratory of Serum Pharmacochemistry, Department of Pharmaceutical Analysis, Laboratory of Metabolomics, Heilongjiang University of Chinese Medicine, Heping Road 24, Harbin 150040, China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Song
authors.affiliation: Sino-America Chinmedomics Technology Cooperation Center, Chinmedomics Research Center of TCM State Administration, National TCM Key Laboratory of Serum Pharmacochemistry, Department of Pharmaceutical Analysis, Laboratory of Metabolomics, Heilongjiang University of Chinese Medicine, Heping Road 24, Harbin 150040, China.|State Key Laboratory of Quality Research in Chinese Medicine, Macau University of Science and Technology, Avenida Wai Long, Taipa, Macau.
authors.forename: Xijun
authors.initials: X
authors.lastname: Wang
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D003374:Coumarins / Q000494:pharmacology / Q000627:therapeutic use*; D004195:Disease Models, Animal; D006801:Humans; D008107:Liver Diseases / Q000188:drug therapy* / Q000378:metabolism* / Q000473:pathology; D053858:Metabolic Networks and Pathways / Q000187:drug effects; D055442:Metabolome / Q000187:drug effects; D008807:Mice, Inbred BALB C; D015999:Multivariate Analysis; D013577:Syndrome; D053719:Tandem Mass Spectrometry
chemical_list: D015415:Biomarkers; D003374:Coumarins; C018145:scoparone