title: Shrimp Protein Hydrolysate Modulates the Timing of Proinflammatory Macrophages in Bupivacaine-Injured Skeletal Muscles in Rats.
abstract: This study was designed to determine whether marine-derived proteins other than cod could have beneficial effects on inflammation following muscle injury. Macrophage and neutrophil densities were measured from bupivacaine-injured tibialis anterior muscle of rats fed isoenergetic diets containing either shrimp hydrolysate (Shr), casein hydrolysate (CaH), or whole casein (Ca). In this study, Shr reduced ED1+-macrophages at day 2 (p= 0.013), day 5 (p= 0.006), and day 14 after injury (p= 0.038) compared with Ca, indicating faster resolution of inflammation in Shr. Except for day 2 after injury where Shr led to lower ED1+-macrophages compared with CaH (p= 0.006), both Shr and CaH responded similarly at days 5, 14, and 28 after injury. This findings suggest that beneficial effects of Shr on ED1+-cells might be related to generation of anti-inflammatory peptides through the hydrolysis process, in addition to its high content of anti-inflammatory amino acids. However, while increasing myofiber cross-sectional area in noninjured muscles compared with both Ca and CaH, Shr failed to have a positive effect in corresponding injured muscles. These data indicate that shrimp hydrolysate can facilitate resolution of inflammation after muscle injury mainly through modulating proinflammatory macrophage accumulation but have less effect on optimal recovery in terms of muscle mass and fiber size.
authors.affiliation: School of Nutrition, Laval University, Quebec City, QC, Canada G1V 0A6; Institute of Nutraceuticals and Functional Foods, Laval University, Quebec City, QC, Canada G1V 0A6.
authors.forename: Junio
authors.identifier: 0000-0002-2381-415X
authors.initials: J
authors.lastname: Dort
authors.affiliation: School of Nutrition, Laval University, Quebec City, QC, Canada G1V 0A6; Institute of Nutraceuticals and Functional Foods, Laval University, Quebec City, QC, Canada G1V 0A6.
authors.forename: Nadine
authors.initials: N
authors.lastname: Leblanc
authors.affiliation: Merinov, Quebec Fisheries and Aquaculture Innovation Centre, 96 montée de Sandy Beach, Office 1.07, Gaspé, QC, Canada G4X 2V6.
authors.forename: Piotr
authors.initials: P
authors.lastname: Bryl
authors.affiliation: ISMER, Université du Québec à Rimouski, 300 allée des Ursulines, P.O. Box 3300, Rimouski, QC, Canada G5L 3A1.
authors.forename: Marie-Gil
authors.initials: MG
authors.lastname: Fortin
authors.affiliation: Merinov, Quebec Fisheries and Aquaculture Innovation Centre, 96 montée de Sandy Beach, Office 1.07, Gaspé, QC, Canada G4X 2V6.
authors.forename: Marie-Elise
authors.initials: ME
authors.lastname: Carbonneau
authors.affiliation: Quebec Agrifood Development Center, La Pocatière, QC, Canada.
authors.forename: Charles
authors.initials: C
authors.lastname: Lavigne
authors.affiliation: School of Nutrition, Laval University, Quebec City, QC, Canada G1V 0A6; Institute of Nutraceuticals and Functional Foods, Laval University, Quebec City, QC, Canada G1V 0A6.
authors.forename: Hélène
authors.identifier: 0000-0002-2755-6346
authors.initials: H
authors.lastname: Jacques
mesh_terms: D000818:Animals; D002045:Bupivacaine / Q000633:toxicity; D002364:Caseins / Q000008:administration & dosage; D007249:Inflammation / Q000188:drug therapy* / Q000503:physiopathology; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism; D018482:Muscle, Skeletal / Q000187:drug effects* / Q000293:injuries / Q000503:physiopathology; D033542:Pandalidae / Q000737:chemistry; D011492:Protein Hydrolysates / Q000008:administration & dosage* / Q000737:chemistry; D051381:Rats; D014947:Wounds and Injuries / Q000139:chemically induced / Q000188:drug therapy* / Q000503:physiopathology
chemical_list: D002364:Caseins; D011492:Protein Hydrolysates; C475729:casein hydrolysate; D002045:Bupivacaine