title: Inhibition ofCronobacter sakazakiibyLactobacillus acidophilusn.v. Er2 317/402.
abstract: Lactobacillus acidophilusn.v. Er2 317/402 strain Narine is known as a health beneficial functional probiotic culture and supplementary source of nutrition for newborns. In this study,in vitroantimicrobial activities of Narine-lyophilized (Narine-L), Narine-heat treated (Narine-HT), and Narine crude cell-free extract (Narine-CCFE) were evaluated against pathogenCronobacter sakazakii(C. sakazakii) in agar as well as in a reconstituted powdered infant formula (RPIF) model. Inhibition zones of 30 mg Narine-L and Narine-HT were both 150 U, whereas inhibition zone of 30 mg Narine-CCFE was 200 U. Narine-L (1 g) and Narine-HT (1 g) were added to 10 mL of artificially contaminated RPIF, respectively, containing 100 μL ofC. sakazakii(1.62×108colony forming unit (CFU)/mL). After treatment with Narine-L and Narine-HT for 3 h and 6 h at 37℃, less than ≤107 CFU/mL ofC. sakazakiiwas detected in RPIF. Without Narine-L and Narine-HT treatment, the population ofC. sakazakiiincreased up to 5.36×109CFU/mL after 6 h. Examination by transmission electron microscopy confirmedC. sakazakiicells were damaged by Narine-CCFE. Thus, employing Narine culture as a natural and safe bio-preservative may protect infants fromC. sakazakii.
authors.affiliation: Department of Food Science and Technology, Yeungnam University, Gyeongsan 38541, Korea.
authors.forename: Haykuhi
authors.initials: H
authors.lastname: Charchoghlyan
authors.affiliation: Department of Food Science and Technology, Yeungnam University, Gyeongsan 38541, Korea.
authors.forename: Heejun
authors.initials: H
authors.lastname: Kwon
authors.affiliation: Department of Food Science and Technology, Yeungnam University, Gyeongsan 38541, Korea.
authors.forename: Dong-Ju
authors.initials: DJ
authors.lastname: Hwang
authors.affiliation: Department of Food and Nutrition and Cook, Taegu Science University, Daegu 41453, Korea.
authors.forename: Jong Suk
authors.initials: JS
authors.lastname: Lee
authors.affiliation: Division of Food and Animal Science, Chungbuk National University, Cheongju 28644, Korea.
authors.forename: Junsoo
authors.initials: J
authors.lastname: Lee
authors.affiliation: Department of Food Science and Technology, Yeungnam University, Gyeongsan 38541, Korea.
authors.forename: Myunghee
authors.initials: M
authors.lastname: Kim
keywords: Cronobacter sakazakii; Lactobacillus acidophilus; antimicrobial activity; infant food