title: Hydrogen Sulfide Regulates the [Ca2+]iLevel in the Primary Medullary Neurons.
abstract: In the present study, we attempted to elucidate mechanisms for the regulation of intracellular calcium levels by H2S in primary rat medullary neurons. Our results showed that NaHS significantly increased the level of [Ca2+]iin rat medullary neurons in a concentration-dependent manner. L-Cysteine and SAM significantly raised the level of [Ca2+]iin the medullary neurons while HA and/or AOAA produced a reversal effect. In addition, L-cysteine and SAM significantly increased but HA and/or AOAA decreased the production of H2S in the cultured neurons. The [Ca2+]ielevation induced by H2S was significantly diminished by EGTA-Ca2+-free solutions, and this elevation was also reduced by nifedipine or nimodipine and mibefradil, suggesting the role of L-type and/or T-type Ca2+channels. Moreover, the effect of H2S on [Ca2+]ilevel in neurons was significantly attenuated by BAPTA-AM and thapsigargin, suggesting the source of Ca2+. Therefore, we concluded that both exogenous and endogenous H2S elevates [Ca2+]ilevel in primarily cultured rat medullary neurons via both increasing calcium influx and mobilizing intracellular Ca2+stores from ER.
authors.affiliation: Department of Physiology and Pathophysiology, Shanghai Medical College, Fudan University, Shanghai 200032, China; Department of Neurology, Huashan Hospital, Fudan University, Shanghai 200040, China.
authors.forename: Xiaoni
authors.initials: X
authors.lastname: Liu
authors.affiliation: Department of Physiology and Pathophysiology, Shanghai Medical College, Fudan University, Shanghai 200032, China.
authors.forename: Nana
authors.initials: N
authors.lastname: Zhang
authors.affiliation: Department of Physiology and Pathophysiology, Shanghai Medical College, Fudan University, Shanghai 200032, China.
authors.forename: Yingjiong
authors.initials: Y
authors.lastname: Ding
authors.affiliation: Department of Neurosurgery, Huashan Hospital, Fudan University, Shanghai 200040, China.
authors.forename: Dongqing
authors.initials: D
authors.lastname: Cao
authors.affiliation: Department of Physiology and Pathophysiology, Shanghai Medical College, Fudan University, Shanghai 200032, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Huang
authors.affiliation: Department of Neurology, Huashan Hospital, Fudan University, Shanghai 200040, China.
authors.forename: Xiangjun
authors.initials: X
authors.lastname: Chen
authors.affiliation: The Cardiovascular and Metabolic Research Unit, Laurentian University, Sudbury, ON, Canada P3E 2C6.
authors.forename: Rui
authors.initials: R
authors.lastname: Wang
authors.affiliation: Department of Physiology and Pathophysiology, Shanghai Medical College, Fudan University, Shanghai 200032, China.
authors.forename: Ning
authors.identifier: 0000-0001-6110-2156
authors.initials: N
authors.lastname: Lu
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism*; D006862:Hydrogen Sulfide / Q000378:metabolism* / Q000494:pharmacology; D051379:Mice; D009474:Neurons / Q000187:drug effects*
chemical_list: D002118:Calcium; D006862:Hydrogen Sulfide