title: Differential Expression ofHoxandNotchGenes in Larval and Adult Stages ofEchinococcus granulosus.
abstract: This investigation aimed to evaluate the differential expression ofHoxB7andnotchgenes in different developmental stages ofEchinococcus granulosussensu stricto. The expression ofHoxB7gene was observed at all developmental stages. Nevertheless, significant fold differences in the expression level was documented in the juvenile worm with 3 or more proglottids, the germinal layer from infected sheep, and the adult worm from an experimentally infected dog. Thenotchgene was expressed at all developmental stages ofE. granulosus; however, the fold difference was significantly increased at the microcysts in monophasic culture medium and the germinal layer of infected sheep in comparison with other stages. The findings demonstrated that the 2 aforementioned genes evaluated in the present study were differentially expressed at different developmental stages of the parasite and may contribute to some important biological processes ofE. granulosus.
authors.affiliation: Research Center for Hydatid Disease in Iran, School of Medicine, Kerman University of Medical Sciences, Kerman 7615614115, Iran.
authors.forename: Ebrahim Saedi
authors.initials: ES
authors.lastname: Dezaki
authors.affiliation: Research Department of Biotechnology, Institute of Sciences and High Technology and Environmental Sciences, Graduate University of Advanced Technology, Kerman, Iran.
authors.forename: Mohammad Mehdi
authors.initials: MM
authors.lastname: Yaghoobi
authors.affiliation: Department of Pathology, School of Medicine, Kerman University of Medical Sciences, Kerman 7615614115, Iran.
authors.forename: Elham
authors.initials: E
authors.lastname: Taheri
authors.affiliation: Research Center for Hydatid Disease in Iran, School of Medicine, Kerman University of Medical Sciences, Kerman 7615614115, Iran.
authors.forename: Pooya Ghaseminejad
authors.initials: PG
authors.lastname: Almani
authors.affiliation: Research Center for Hydatid Disease in Iran, School of Medicine, Kerman University of Medical Sciences, Kerman 7615614115, Iran.
authors.forename: Farideh
authors.initials: F
authors.lastname: Tohidi
authors.affiliation: Institute of Parasitology, Faculty of Medicine and Vetsuisse Faculty of the University of Bern, Bern, Switzerland.
authors.forename: Bruno
authors.initials: B
authors.lastname: Gottstein
authors.affiliation: Research Center for Hydatid Disease in Iran, School of Medicine, Kerman University of Medical Sciences, Kerman 7615614115, Iran.
authors.forename: Majid Fasihi
authors.initials: MF
authors.lastname: Harandi
mesh_terms: D000818:Animals; D004285:Dogs; D048209:Echinococcus granulosus / Q000235:genetics* / Q000254:growth & development / Q000302:isolation & purification; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D018398:Homeodomain Proteins / Q000096:biosynthesis* / Q000235:genetics; D006801:Humans; D008297:Male; D051880:Receptors, Notch / Q000096:biosynthesis* / Q000235:genetics; D012756:Sheep
chemical_list: D018398:Homeodomain Proteins; D051880:Receptors, Notch
keywords: Echinococcus granulosus; HoxB7 gene; cystic echinococcosis; notch gene; real-time PCR