title: Expression Analysis of the Stem Cell Marker Pw1/Peg3 Reveals a CD34 Negative Progenitor Population in the Hair Follicle.
abstract: Pw1/Peg3 is a parentally imprinted gene expressed in adult stem cells in every tissue thus far examined including the stem cells of the hair follicle. Using a Pw1/Peg3 reporter mouse, we carried out a detailed dissection of the stem cells in the bulge, which is a major stem cell compartment of the hair follicle in mammalian skin. We observed that PW1/Peg3 expression initiates upon placode formation during fetal development, coincident with the establishment of the bulge stem cells. In the adult, we observed that PW1/Peg3 expression is found in both CD34+ and CD34- populations of bulge stem cells. We demonstrate that both populations can give rise to new hair follicles, reconstitute their niche, and self-renew. These results demonstrate that PW1/Peg3 is a reliable marker of the full population of follicle stem cells and reveal a novel CD34- bulge stem-cell population. Stem Cells 2017;35:1015-1027.
authors.affiliation: Stem Cells and Regenerative Medicine, Institute of Cardiometabolism and Nutrition (ICAN), UMRS 1166 INSERM, University of Pierre and Marie Curie Paris VI, Paris, France.
authors.forename: Vanessa
authors.initials: V
authors.lastname: Besson
authors.affiliation: Stem Cells and Regenerative Medicine, Institute of Cardiometabolism and Nutrition (ICAN), UMRS 1166 INSERM, University of Pierre and Marie Curie Paris VI, Paris, France.
authors.forename: Sergiy
authors.initials: S
authors.lastname: Kyryachenko
authors.affiliation: Institute for Research in Biomedicine (IRB Barcelona), The Barcelona Institute of Science and Technology, Barcelona, Spain.
authors.forename: Peggy
authors.initials: P
authors.lastname: Janich
authors.affiliation: Institute for Research in Biomedicine (IRB Barcelona), The Barcelona Institute of Science and Technology, Barcelona, Spain.|ICREA, Catalan Institution for Research and Advanced Studies, Barcelona, Spain.|Institució Catalana de Recerca i Estudis Avançats (ICREA), Barcelona, Spain.
authors.forename: Salvador A
authors.initials: SA
authors.lastname: Benitah
authors.affiliation: Stem Cells and Regenerative Medicine, Institute of Cardiometabolism and Nutrition (ICAN), UMRS 1166 INSERM, University of Pierre and Marie Curie Paris VI, Paris, France.
authors.forename: Giovanna
authors.initials: G
authors.lastname: Marazzi
authors.affiliation: Stem Cells and Regenerative Medicine, Institute of Cardiometabolism and Nutrition (ICAN), UMRS 1166 INSERM, University of Pierre and Marie Curie Paris VI, Paris, France.
authors.forename: David
authors.initials: D
authors.lastname: Sassoon
mesh_terms: D000375:Aging / Q000502:physiology; D000818:Animals; D000831:Animals, Newborn; D018952:Antigens, CD34 / Q000378:metabolism*; D015415:Biomarkers / Q000378:metabolism; D000066673:Cell Self Renewal; D002469:Cell Separation; D015870:Gene Expression; D017930:Genes, Reporter; D018859:Hair Follicle / Q000166:cytology*; D051741:Kruppel-Like Transcription Factors / Q000378:metabolism*; D008810:Mice, Inbred C57BL; D013194:Staining and Labeling; D055153:Stem Cell Niche; D013234:Stem Cells / Q000166:cytology* / Q000378:metabolism*
chemical_list: D018952:Antigens, CD34; D015415:Biomarkers; D051741:Kruppel-Like Transcription Factors; C097901:Peg3 protein, mouse
keywords: Bulge stem cells; Cell cycle; Pw1/Peg3