title: Assessment of the anti-feeding and insecticidal effects of the combination of dinotefuran, permethrin and pyriproxyfen (Vectra®3D) against Triatoma infestans on rats.
abstract: This study, based on the rat model, was designed to explore the anti-feeding and insecticidal efficacy of a topical ectoparasiticide, dinotefuran-permethrin-pyriproxyfen (DPP), against Triatoma infestans (Hemiptera: Reduviidae), a vector of Trypanosoma cruzi (Trypanosomatida: Trypanosomatidae), for which dogs are domestic reservoir hosts. Twenty rats were divided into two equal groups: untreated and treated. Each rat was exposed under sedation to 16 T. infestans of mixed life stages for 1 h on days 1, 7, 14, 21 and 28 post-treatment. The anti-feeding and insecticidal effects of DPP were estimated after 1 h of exposure. Insecticidal efficacy was also assessed after incubation of the insects for 24 h post-exposure. Anti-feeding efficacy was 96.7, 84.7, 80.5, 81.5 and 42.6% on days 1, 7, 14, 21 and 28, respectively. Insecticidal efficacy evaluated at 1 and 24 h after exposure on days 1, 7, 14, 21 and 28 was 100, 91.2, 82.5, 80.0 and 29.1, and 100, 100, 100, 96.0 and 49.9%, respectively. This study demonstrates that a single administration of DPP spot-on treatment at a dose equivalent to the minimal recommended dose in rats has a powerful effect against T. infestans starting from day 1 that lasts for at least 3 weeks.
authors.affiliation: Unité de Recherche en Maladies Infectieuses et Tropicales Emergentes (URMITE), UM63, Centre National de la Recherche Scientifique (CNRS) 7278, Institut de Recherche pour le Développement (IRD) 198 (Dakar), Institut National de la Santé et de la Recherche Médicale (INSERM) 1095, Aix-Marseille University, Marseille, France.
authors.forename: D
authors.initials: D
authors.lastname: Tahir
authors.affiliation: Unité de Recherche en Maladies Infectieuses et Tropicales Emergentes (URMITE), UM63, Centre National de la Recherche Scientifique (CNRS) 7278, Institut de Recherche pour le Développement (IRD) 198 (Dakar), Institut National de la Santé et de la Recherche Médicale (INSERM) 1095, Aix-Marseille University, Marseille, France.
authors.forename: B
authors.initials: B
authors.lastname: Davoust
authors.affiliation: Ceva Santé Animale SA, Libourne, France.
authors.forename: M
authors.initials: M
authors.lastname: Varloud
authors.affiliation: Unité de Recherche en Maladies Infectieuses et Tropicales Emergentes (URMITE), UM63, Centre National de la Recherche Scientifique (CNRS) 7278, Institut de Recherche pour le Développement (IRD) 198 (Dakar), Institut National de la Santé et de la Recherche Médicale (INSERM) 1095, Aix-Marseille University, Marseille, France.
authors.forename: J-M
authors.initials: JM
authors.lastname: Berenger
authors.affiliation: Unité de Recherche en Maladies Infectieuses et Tropicales Emergentes (URMITE), UM63, Centre National de la Recherche Scientifique (CNRS) 7278, Institut de Recherche pour le Développement (IRD) 198 (Dakar), Institut National de la Santé et de la Recherche Médicale (INSERM) 1095, Aix-Marseille University, Marseille, France.
authors.forename: D
authors.initials: D
authors.lastname: Raoult
authors.affiliation: Unité de Recherche en Maladies Infectieuses et Tropicales Emergentes (URMITE), UM63, Centre National de la Recherche Scientifique (CNRS) 7278, Institut de Recherche pour le Développement (IRD) 198 (Dakar), Institut National de la Santé et de la Recherche Médicale (INSERM) 1095, Aix-Marseille University, Marseille, France.
authors.forename: L
authors.initials: L
authors.lastname: Almeras
authors.affiliation: Unité de Recherche en Maladies Infectieuses et Tropicales Emergentes (URMITE), UM63, Centre National de la Recherche Scientifique (CNRS) 7278, Institut de Recherche pour le Développement (IRD) 198 (Dakar), Institut National de la Santé et de la Recherche Médicale (INSERM) 1095, Aix-Marseille University, Marseille, France.
authors.forename: P
authors.initials: P
authors.lastname: Parola
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D004478:Ectoparasitic Infestations / Q000517:prevention & control / Q000662:veterinary*; D005260:Female; D006146:Guanidines; D007300:Insect Control; D007306:Insecticides; D008297:Male; D000073943:Neonicotinoids; D009574:Nitro Compounds; D009758:Nymph / Q000254:growth & development; D026023:Permethrin; D011725:Pyridines; D051381:Rats; D014224:Triatoma / Q000254:growth & development
chemical_list: D006146:Guanidines; D007306:Insecticides; D000073943:Neonicotinoids; D009574:Nitro Compounds; D011725:Pyridines; C465368:dinotefuran; C055613:pyriproxyfen; D026023:Permethrin
keywords: Triatoma infestans; Trypanosoma cruzi; ectoparasiticide; efficacy; rat model