title: Biosensing breast cancer cells based on a three-dimensional TIO2nanomembrane transducer.
abstract: The early diagnosis of breast cancer is crucial for the successful treatment and recovery phases of the patients suffering from the disease. Although mammography is considered the gold standard for diagnosis, it fails to detect some cancers in high-density breasts. In this work, we propose for the first time a tridimensional biosensor platform, to be used on an electrochemical point-of-care device. The bioconjugated platform is constructed on a series of covalent linkages between lectin molecules and a cysteine layer immobilized over gold-coated TiO2butterfly-like tridimensional nanomembranes. Through the use of vegetal lectins, we managed to take advantage of the markedly atypical glycomic profile of the cancerous mammalian cell membrane and successfully made a distinction between highly invasive (T47D) and less invasive (MCF7) cancer cell lines. The selectivity of the biosensor was tested by using normal human skin-fibroblast. The proposed cytosensor demonstrated limits of detection as low as 10 cells mL-1for every cell line and a linear range from 10 to 1.0×106cells mL-1. Considering that electrochemical impedance values can be correlated with the number of breast cancer cells present in the sample, we suggest that the proposed platform could be useful in facilitating the diagnosis of cancer.
authors.affiliation: Programa de Pós-Graduação em Inovação Terapêutica, Universidade Federal de Pernambuco, 50670-901 Recife, PE, Brasil.
authors.forename: Fernando
authors.initials: F
authors.lastname: Zanghelini
authors.affiliation: Programa de Pós-Graduação em Inovação Terapêutica, Universidade Federal de Pernambuco, 50670-901 Recife, PE, Brasil.
authors.forename: Isaac A M
authors.initials: IAM
authors.lastname: Frías
authors.affiliation: Programa de Pós-Graduação em Inovação Terapêutica, Universidade Federal de Pernambuco, 50670-901 Recife, PE, Brasil.
authors.forename: Moacyr J B M
authors.initials: MJBM
authors.lastname: Rêgo
authors.affiliation: Programa de Pós-Graduação em Inovação Terapêutica, Universidade Federal de Pernambuco, 50670-901 Recife, PE, Brasil.
authors.forename: Maira G R
authors.initials: MGR
authors.lastname: Pitta
authors.affiliation: Departamento de Física, Universid ade Federal de Pernambuco, 50670-901 Recife, PE, Brasil.
authors.forename: Marco
authors.initials: M
authors.lastname: Sacilloti
authors.affiliation: Departamento de Bioquímica, Universidade Federal de Pernambuco, 50670-901 Recife, PE, Brasil.
authors.forename: Maria D L
authors.initials: MDL
authors.lastname: Oliveira
authors.affiliation: Programa de Pós-Graduação em Inovação Terapêutica, Universidade Federal de Pernambuco, 50670-901 Recife, PE, Brasil; Departamento de Bioquímica, Universidade Federal de Pernambuco, 50670-901 Recife, PE, Brasil. Electronic address: csrandrade@gmail.com.
authors.forename: Cesar A S
authors.initials: CAS
authors.lastname: Andrade
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D001940:Breast / Q000473:pathology*; D001943:Breast Neoplasms / Q000175:diagnosis*; D045744:Cell Line, Tumor; D003545:Cysteine / Q000737:chemistry; D058266:Dielectric Spectroscopy / Q000295:instrumentation / Q000379:methods*; D005260:Female; D006801:Humans; D055767:Immobilized Proteins / Q000737:chemistry; D037102:Lectins / Q000737:chemistry; D008567:Membranes, Artificial; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure; D019095:Point-of-Care Systems; D014025:Titanium / Q000737:chemistry*; D014159:Transducers
chemical_list: D055767:Immobilized Proteins; D037102:Lectins; D008567:Membranes, Artificial; C009495:titanium dioxide; D014025:Titanium; D003545:Cysteine
keywords: Biosensor; Cancer cells; Impedance spectroscopy; Lectins