title: Neuroprotective potential of curcumin in combination with piperine against 6-hydroxy dopamine induced motor deficit and neurochemical alterations in rats.
abstract: OBJECTIVE
  6-hydroxy dopamine (6-OHDA) is a neurotoxin which on intranigral administration produces severe nigrostriatal damage with motor and cognitive deficit in animals. Curcumin (CMN) in combination with bioenhancer piperine (PP) in 6-hydroxydopamine-induced Parkinsonian rats was used to investigate the antioxidant, neuromodulatory and neuroprotective mechanisms.
  
  
  METHODS
  Hemi-Parkinson's rat model was developed with intranigral infusion of 6-OHDA (8 μg/2 μl, once, unilaterally), treatment with CMN (25 and 50 mg/kg) and combination of PP (2.5 mg/kg) with CMN (25 mg/kg) was given daily for 21 days starting from the 7th day after 6-OHDA infusion. The behavioral (locomotor, grip strength, and narrow beam walk) parameters were studied on weekly basis. On 22nd day, isolated brain preparations were subjected to biochemical (lipid peroxidation, glutathione, and nitrite), neuroinflammatory (IL-1β, IL-6, and TNF- α), and neurochemical (DA, NE, 5- HT, GABA, Glutamate, DOPAC, HVA, and 5-HIAA) analysis.
  
  
  RESULTS
  Oral administration of CMN had significantly prevented behavioral, neuroinflammatory, and neurochemical changes and preserved the antioxidant potential of the nigrostriatum in rats treated with 6-OHDA.
  
  
  CONCLUSIONS
  In the present study, PP and CMN had afforded a better neuroprotective effect compared to alone treatment on behavior, biochemical, neuroinflammatory, and neurochemical parameters in rats.
authors.affiliation: Department of Pharmacology, I.S.F College of Pharmacy, Ferozepur Road, Ghal Kalan, Moga, 142001, Punjab, India.|Research Scholar, I.K. Gujral Punjab Technical University, Jalandhar, India.
authors.forename: Shamsher
authors.initials: S
authors.lastname: Singh
authors.affiliation: Department of Pharmacology, I.S.F College of Pharmacy, Ferozepur Road, Ghal Kalan, Moga, 142001, Punjab, India. punnubansal79@gmail.com.
authors.forename: Puneet
authors.initials: P
authors.lastname: Kumar
mesh_terms: D000470:Alkaloids / Q000008:administration & dosage*; D000818:Animals; D052117:Benzodioxoles / Q000008:administration & dosage*; D003342:Corpus Striatum / Q000737:chemistry* / Q000187:drug effects / Q000378:metabolism; D003474:Curcumin / Q000008:administration & dosage*; D004359:Drug Therapy, Combination; D018737:Hand Strength / Q000502:physiology; D008124:Locomotion / Q000187:drug effects / Q000502:physiology; D008297:Male; D019957:Motor Skills Disorders / Q000139:chemically induced / Q000378:metabolism / Q000517:prevention & control*; D018696:Neuroprotective Agents / Q000008:administration & dosage*; D016627:Oxidopamine / Q000633:toxicity*; D010880:Piperidines / Q000008:administration & dosage*; D053284:Polyunsaturated Alkamides / Q000008:administration & dosage*; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar
chemical_list: D000470:Alkaloids; D052117:Benzodioxoles; D018696:Neuroprotective Agents; D010880:Piperidines; D053284:Polyunsaturated Alkamides; D016627:Oxidopamine; D003474:Curcumin; C008922:piperine
keywords: 6-OHDA; Curcumin; Neuroinflammation; Oxidative stress; Parkinson’s disease; Piperine