title: Increased TIM3+CD8+T cells in Myelodysplastic Syndrome patients displayed less perforin and granzyme B secretion and higher CD95 expression.
abstract: T cell immunoglobulin and mucin domain 3(TIM3) is a negative regulator of cellular immunity and it is highly expressed on CD8+T cells in persistent viral infection and cancer setting as report. However, how TIM3 expressed on CD8+T cells in myelodysplastic syndrome (MDS), that is a malignant disorder, has not been clarified. Here, decreased CD8+T cells, less IFN-γ secretion in CD8+T cells and increased TIM3 on CD8+T cells had been seen. Increased TIM3+CD8+T cells with lower perforin and granzyme B expression and higher CD95 expression in MDS patients had been observed. These findings suggested that TIM3 might be related to CD8+T cells defect. Therefore, further explorations about mechanism of TIM3+CD8+T cells defect are needed, which might be helpful for adoptive T-cell therapy in MDS.
authors.affiliation: Hematology department of Tianjin, Medical University General Hospital, Tianjin 300052, China.
authors.forename: Jinglian
authors.initials: J
authors.lastname: Tao
authors.affiliation: Hematology department of Tianjin, Medical University General Hospital, Tianjin 300052, China.
authors.forename: Lijuan
authors.initials: L
authors.lastname: Li
authors.affiliation: Hematology department of Tianjin, Medical University General Hospital, Tianjin 300052, China.
authors.forename: Yingshuai
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Hematology department of Tianjin, Medical University General Hospital, Tianjin 300052, China.
authors.forename: Rong
authors.initials: R
authors.lastname: Fu
authors.affiliation: Hematology department of Tianjin, Medical University General Hospital, Tianjin 300052, China.
authors.forename: Huaquan
authors.initials: H
authors.lastname: Wang
authors.affiliation: Hematology department of Tianjin, Medical University General Hospital, Tianjin 300052, China. Electronic address: shaozonghong@sina.com.
authors.forename: Zonghong
authors.initials: Z
authors.lastname: Shao
mesh_terms: D000328:Adult; D018414:CD8-Positive T-Lymphocytes / Q000737:chemistry* / Q000378:metabolism / Q000473:pathology; D002478:Cells, Cultured; D005260:Female; D053804:Granzymes / Q000378:metabolism*; D000072597:Hepatitis A Virus Cellular Receptor 2 / Q000032:analysis*; D006801:Humans; D007111:Immunity, Cellular; D007371:Interferon-gamma / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D009190:Myelodysplastic Syndromes / Q000473:pathology*; D054353:Perforin / Q000378:metabolism*; D019014:fas Receptor / Q000032:analysis*
chemical_list: C491901:FAS protein, human; C448908:HAVCR2 protein, human; D000072597:Hepatitis A Virus Cellular Receptor 2; D019014:fas Receptor; D054353:Perforin; D007371:Interferon-gamma; C509213:GZMB protein, human; D053804:Granzymes
keywords: CD8+T cells; Myelodysplastic syndrome; T cell immunoglobulin and mucin domain 3