title: In VivoAngiogenic Capacity of Stem Cells from Human Exfoliated Deciduous Teeth with Human Umbilical Vein Endothelial Cells.
abstract: Dental pulp is a highly vascularized tissue requiring adequate blood supply for successful regeneration. In this study, we investigated the functional role of stem cells from human exfoliated deciduous teeth (SHEDs) as a perivascular source forin vivoformation of vessel-like structures. Primarily isolated SHEDs showed mesenchymal stem cell (MSC)-like characteristics including the expression of surface antigens andin vitroosteogenic and adipogenic differentiation potentials. Moreover, SHEDs were positive for NG2, α-smooth muscle actin (SMA), platelet-derived growth factor receptor beta (PDGFRβ), and CD146 as pericyte markers. To prove feasibility of SHEDs as perivascular source, SHEDs were transplanted into immunodeficient mouse using Matrigel with or without human umbilical vein endothelial cells (HUVECs). Transplantation of SHEDs alone or HUVECs alone resulted in no formation of vessel-like structures with enough red blood cells. However, when SHEDs and HUVECs were transplanted together, extensive vessel-like structures were formed. The presence of murine erythrocytes within lumens suggested the formation of anastomoses between newly formed vessel-like structures in Matrigel plug and the host circulatory system. To understand underlying mechanisms ofin vivoangiogenesis, the expression of angiogenic cytokine and chemokine, their receptors, and MMPs was compared between SHEDs and HUVECs. SHEDs showed higher expression of VEGF, SDF-1α, and PDGFRβ than HUVECs. On the contrary, HUVECs showed higher expression of VEGF receptors, CXCR4, and PDGF-BB than SHEDs. This differential expression pattern suggested reciprocal interactions between SHEDs and HUVECs and their involvement duringin vivoangiogenesis. In conclusion, SHEDs could be a feasible source of perivascular cells forin vivoangiogenesis.
authors.affiliation: Laboratory of Molecular Genetics, Dental Research Institute, School of Dentistry, Seoul National University, Seoul 03080, Korea.
authors.forename: Ji-Hye
authors.initials: JH
authors.lastname: Kim
authors.affiliation: Laboratory of Molecular Genetics, Dental Research Institute, School of Dentistry, Seoul National University, Seoul 03080, Korea.
authors.forename: Gee-Hye
authors.initials: GH
authors.lastname: Kim
authors.affiliation: Laboratory of Molecular Genetics, Dental Research Institute, School of Dentistry, Seoul National University, Seoul 03080, Korea.
authors.forename: Jae-Won
authors.initials: JW
authors.lastname: Kim
authors.affiliation: Department of Anatomy and Cell Biology, Sungkyunkwan University School of Medicine, Seoul 06351, Korea.|Stem Cell and Regenerative Medicine Center, Research Institute for Future Medicine, Samsung Medical Center, Seoul 06351, Korea.
authors.forename: Hee Jang
authors.initials: HJ
authors.lastname: Pyeon
authors.affiliation: Children's Dental Center and CDC Baby Tooth Stem Cell Bank, Seoul 06072, Korea.
authors.forename: Jae Cheoun
authors.initials: JC
authors.lastname: Lee
authors.affiliation: Laboratory of Molecular Genetics, Dental Research Institute, School of Dentistry, Seoul National University, Seoul 03080, Korea.
authors.forename: Gene
authors.initials: G
authors.lastname: Lee
authors.affiliation: Stem Cell and Regenerative Medicine Center, Research Institute for Future Medicine, Samsung Medical Center, Seoul 06351, Korea.|Department of Neurosurgery, Samsung Medical Center, Sungkyunkwan University, Seoul 06351, Korea.
authors.forename: Hyun
authors.initials: H
authors.lastname: Nam
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D003782:Dental Pulp / Q000166:cytology* / Q000378:metabolism; D061307:Human Umbilical Vein Endothelial Cells / Q000166:cytology* / Q000378:metabolism; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism; D051379:Mice; D018919:Neovascularization, Physiologic / Q000502:physiology; D014094:Tooth, Deciduous / Q000166:cytology* / Q000378:metabolism
keywords: SHEDs; in vivo angiogenesis; mesenchymal stem cell; pericyte; tissue engineering