title: Banking of Adipose- and Cord Tissue-Derived Stem Cells: Technical and Regulatory Issues.
abstract: Stem cells are found in all multicellular organisms and are defined as cells that can differentiate into specialized mature cells as well as divide to produce more stem cells. Mesenchymal stem cells (MSC) were among the first stem cell types to be utilized for regenerative medicine. Although initially isolated from bone marrow, based on ease and costs of procurement, MSC derived from adipose tissue (AT-MSC) and umbilical cord tissue (CT-MSC) are now preferred stem cell sources for these applications. Both adipose tissues and cord tissue present unique problems for biobanking however, in that these are whole tissues, not cellular suspensions. Although the tissues could be processed to facilitate the biobanking process, by doing so additional regulatory issues arise that must be addressed. This review will discuss the technical issues associated with biobanking of these tissues, as well as regulatory concerns when banking of utilizing MSC derived from these sources in the clinic.
authors.affiliation: University of Arizona Health Sciences Biorepository, Tucson, AZ, USA. davidh@email.arizona.edu.|University of Arizona, Tucson, AZ, USA. davidh@email.arizona.edu.|Division of Translational Medicine, University of Arizona, Tucson, AZ, USA. davidh@email.arizona.edu.|GMP Laboratory at the University of Arizona, Tucson, AZ, USA. davidh@email.arizona.edu.|Celebration Stem Cell Centre, Gilbert, AZ, USA. davidh@email.arizona.edu.
authors.forename: David T
authors.initials: DT
authors.lastname: Harris
mesh_terms: D017667:Adipocytes / Q000166:cytology* / Q000187:drug effects / Q000502:physiology; D000273:Adipose Tissue / Q000166:cytology* / Q000187:drug effects / Q000502:physiology; D018070:Biological Specimen Banks / Q000941:ethics / Q000331:legislation & jurisprudence*; D002454:Cell Differentiation; D015925:Cryopreservation / Q000379:methods*; D003451:Cryoprotective Agents / Q000494:pharmacology; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D017277:Drug Approval / Q000331:legislation & jurisprudence*; D005312:Fetal Blood / Q000166:cytology / Q000502:physiology; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000502:physiology; D044968:Regenerative Medicine / Q000379:methods
chemical_list: D003451:Cryoprotective Agents; D004121:Dimethyl Sulfoxide
keywords: Adipose tissue; Biobanking; Cord tissue; FDA; Regenerative medicine; Regulations; Stem cells