title: Prevalence and antibiotic resistance profile of thermophilicCampylobacterspp. of slaughtered cattle and sheep in Shiraz, Iran
abstract: Although poultry meat is considered as the main source for humanCampylobacterinfections, there is limited information about non-poultry sources. The present study was aimed to investigate the prevalence and the antibiotic resistance of thermophilicCampylobacterspp. in fecal samples of the cattle and sheep in Shiraz, Iran. A total of 302 fecal samples were obtained from clinically healthy, slaughtered cattle and sheep from Shiraz slaughterhouse. The animals were clinically healthy before being slaughtered. The samples were cultured according to the specific cultivation method under thermophilic conditions. The susceptibility ofCampylobacterisolates were determined for 13 antimicrobial agents. All enriched samples and cultured isolates were targeted for polymerase chain reaction (PCR) detection of16S rRNAand multiplex PCR for determining their species. Among 302 fecal samples, 65 (21.5%) and 205 (67.8%) samples were positive for the presence ofCampylobacterspecies with the cultivation and PCR techniques, respectively. All 65 distinct isolates were susceptible to neomycin and colistin and the isolates showed high resistance to cephalotin (83.0%) and ciprofloxacin (67.7%). After the multiplex PCR, 78.5% of total positive samples showed the simultaneous presence ofCampylobacter jejuniandCampylobacter coli. In conclusion, the results emphasized that non-poultry farms are important as a possible source ofCampylobacterinfections.
authors.affiliation: Department of Food Hygiene, Faculty of Veterinary Medicine, Amol University of Special Modern Technologies, Amol, Iran.
authors.forename: Rahem
authors.initials: R
authors.lastname: Khoshbakht
authors.affiliation: Department of Pathobiology, School of Veterinary Medicine, Shiraz University, Shiraz, Iran.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Tabatabaei
authors.affiliation: Department of Food Hygiene, School of Veterinary Medicine, Shiraz University, Shiraz, Iran.
authors.forename: Saeid
authors.initials: S
authors.lastname: Hoseinzadeh
authors.affiliation: Department of Public Health, School of Health, Golestan University of Medical Sciences, Gorgan, Iran.
authors.forename: Mojtaba
authors.initials: M
authors.lastname: Raeisi
authors.affiliation: Department of Pathobiology, School of Veterinary Medicine, Shiraz University, Shiraz, Iran.
authors.forename: Hesamaddin
authors.initials: H
authors.lastname: Shirzad Aski
authors.affiliation: Department of Food Hygiene, School of Veterinary Medicine, Shiraz University, Shiraz, Iran.
authors.forename: Enayat
authors.initials: E
authors.lastname: Berizi
keywords: Antibiotic resistance; Cattle; PCR; Sheep; Thermophilic Campylobacter spp.