title: Functional and immuno-reactive characterization of a previously undescribed peptide from the venom of the scorpion Centruroides limpidus.
abstract: A previously undescribed toxic peptide named Cl13 was purified from the venom of the Mexican scorpion Centruroides limpidus. It contains 66 amino acid residues, including four disulfide bonds. The physiological effects assayed in 7 different subtypes of voltage gated Na+-channels, showed that it belongs to the β-scorpion toxin type. The most notorious effects were observed in subtypes Nav1.4, Nav1.5 and Nav1.6. Although having important sequence similarities with two other lethal toxins from this scorpion species (Cll1m and Cll2), the recently developed single chain antibody fragments (scFv) of human origin were not capable of protecting against Cl13. At the amino acid sequence level, in 3 stretches of peptide Cl13 (positions 7-9, 30-38 and 62-66) some differences with respect to other similar toxins are observed. Some of these differences coincide with contact points with the human antibody fragments.
authors.affiliation: Departamento de Medicina Molecular, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Avenida Universidad, 2001, Apartado Postal 510.3 Cuernavaca, Morelos, 62210, Mexico.
authors.forename: Timoteo
authors.initials: T
authors.lastname: Olamendi-Portugal
authors.affiliation: Departamento de Medicina Molecular, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Avenida Universidad, 2001, Apartado Postal 510.3 Cuernavaca, Morelos, 62210, Mexico.
authors.forename: Rita
authors.initials: R
authors.lastname: Restano-Cassulini
authors.affiliation: CONACYT, Instituto de Biotecnología,Universidad Nacional Autónoma de México, Mexico.
authors.forename: Lidia
authors.initials: L
authors.lastname: Riaño-Umbarila
authors.affiliation: Departamento de Medicina Molecular, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Avenida Universidad, 2001, Apartado Postal 510.3 Cuernavaca, Morelos, 62210, Mexico.
authors.forename: Baltazar
authors.initials: B
authors.lastname: Becerril
authors.affiliation: Departamento de Medicina Molecular, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Avenida Universidad, 2001, Apartado Postal 510.3 Cuernavaca, Morelos, 62210, Mexico. Electronic address: possani@ibt.unam.mx.
authors.forename: Lourival D
authors.initials: LD
authors.lastname: Possani
mesh_terms: D000595:Amino Acid Sequence / Q000235:genetics; D000818:Animals; D006801:Humans; D010455:Peptides / Q000737:chemistry / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D012604:Scorpion Venoms / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D012605:Scorpions / Q000737:chemistry / Q000235:genetics / Q000276:immunology; D016415:Sequence Alignment; D057127:Single-Chain Antibodies / Q000276:immunology; D061566:Voltage-Gated Sodium Channels / Q000235:genetics / Q000276:immunology* / Q000378:metabolism
chemical_list: D010455:Peptides; D012604:Scorpion Venoms; D057127:Single-Chain Antibodies; D061566:Voltage-Gated Sodium Channels
keywords: Antibody; Centruroides limpidus; Scorpion venom; toxinToxin