title: Wnt-β-Catenin Signaling Promotes the Maturation of Mast Cells.
abstract: Mast cells play an important role in the pathogenesis of allergic diseases. Immature mast cells migrate into peripheral tissues from the bone marrow and undergo complete maturation. Interestingly, mast cells have characteristics similar to hematopoietic stem cells (HSCs), such as self-renewal and c-kit expression. In HSCs, Wnt signaling is involved in their maintenance and differentiation. On the other hand, the relation between Wnt signaling and mast cell differentiation is poorly understood. To study whether Wnt signals play a role in the maturation of mast cells, we studied the effect of Wnt proteins on mast cell maturation of bone marrow-derived mast cells (BMMCs). The expression levels of CD81 protein and histidine decarboxylase mRNA and activity of mast cell-specific protease were all elevated in BMMCs treated with Wnt5a. In addition, Wnt5a induced the expression of Axin2 and TCF mRNA in BMMCs. These results showed that Wnt5a could promote the maturation of mast cells via the canonical Wnt signaling pathway and provide important insights into the molecular mechanisms underlying the differentiation of mast cells.
authors.affiliation: Laboratory of Stem Cell Regulation, National Institutes of Biomedical Innovation, Health and Nutrition, Saito-Asagi 7-6-8, Ibaraki, Osaka 567-0085, Japan.
authors.forename: Tomoko
authors.identifier: 0000-0003-1223-6750
authors.initials: T
authors.lastname: Yamaguchi
authors.affiliation: Laboratory of Stem Cell Regulation, National Institutes of Biomedical Innovation, Health and Nutrition, Saito-Asagi 7-6-8, Ibaraki, Osaka 567-0085, Japan.
authors.forename: Misae
authors.initials: M
authors.lastname: Nishijima
authors.affiliation: Laboratory of Stem Cell Regulation, National Institutes of Biomedical Innovation, Health and Nutrition, Saito-Asagi 7-6-8, Ibaraki, Osaka 567-0085, Japan.
authors.forename: Katsuhisa
authors.initials: K
authors.lastname: Tashiro
authors.affiliation: Laboratory of Stem Cell Regulation, National Institutes of Biomedical Innovation, Health and Nutrition, Saito-Asagi 7-6-8, Ibaraki, Osaka 567-0085, Japan; Laboratory of Biomedical Innovation, Graduate School of Pharmaceutical Sciences, Osaka University, Yamadaoka 1-6, Suita, Osaka 565-0871, Japan.
authors.forename: Kenji
authors.identifier: 0000-0003-1850-2597
authors.initials: K
authors.lastname: Kawabata
mesh_terms: D000818:Animals; D060466:Axin Protein / Q000096:biosynthesis; D001854:Bone Marrow Cells / Q000378:metabolism; D002454:Cell Differentiation / Q000235:genetics*; D018507:Gene Expression Regulation, Developmental; D006640:Histidine Decarboxylase / Q000096:biosynthesis; D006967:Hypersensitivity / Q000235:genetics* / Q000473:pathology; D008407:Mast Cells / Q000166:cytology / Q000378:metabolism*; D051379:Mice; D060225:Tetraspanin 28 / Q000096:biosynthesis; D060449:Wnt Signaling Pathway / Q000235:genetics; D000071818:Wnt-5a Protein / Q000008:administration & dosage / Q000235:genetics* / Q000378:metabolism
chemical_list: D060466:Axin Protein; C111729:Axin2 protein, mouse; D060225:Tetraspanin 28; D000071818:Wnt-5a Protein; D006640:Histidine Decarboxylase