title: MAN1 Restricts BMP Signaling During Synaptic Growth in Drosophila.
abstract: Bone morphogenic protein (BMP) signaling is crucial for coordinated synaptic growth and plasticity. Here, we show that the nuclear LEM-domain protein MAN1 is a negative regulator of synaptic growth at Drosophila larval and adult neuromuscular junctions (NMJs). Loss of MAN1 is associated with synaptic structural defects, including floating T-bars, membrane attachment defects, and accumulation of vesicles between perisynaptic membranes and membranes of the subsynaptic reticulum. In addition, MAN1 mutants accumulate more heterogeneously sized vesicles and multivesicular bodies in larval and adult synapses, the latter indicating that MAN1 may function in synaptic vesicle recycling and endosome-to-lysosome trafficking. Synaptic overgrowth in MAN1 is sensitive to BMP signaling levels, and loss of key BMP components attenuate BMP-induced synaptic overgrowth. Based on these observations, we propose that MAN1 negatively regulates accumulation and distribution of BMP signaling components to ensure proper synaptic growth and integrity at larval and adult NMJs.
authors.affiliation: Department of Molecular Structural Biology, Max Planck Institute of Biochemistry, Martinsried, Germany.
authors.forename: Ulrike
authors.initials: U
authors.lastname: Laugks
authors.affiliation: Neurobiology and Genetics, Biocenter, Theodor-Boveri Institute, Julius-Maximilians University Wuerzburg, Wuerzburg, Germany.
authors.forename: Marie
authors.initials: M
authors.lastname: Hieke
authors.affiliation: Institute of Anatomy and Cell Biology, Julius-Maximilians University Wuerzburg, Wuerzburg, Germany. nicole.wagner@uni-wuerzburg.de.
authors.forename: Nicole
authors.identifier: http://orcid.org/0000-0002-1049-4062
authors.initials: N
authors.lastname: Wagner
mesh_terms: D000818:Animals; D019485:Bone Morphogenetic Proteins / Q000378:metabolism*; D029721:Drosophila Proteins / Q000235:genetics / Q000378:metabolism*; D004331:Drosophila melanogaster / Q000378:metabolism*; D007814:Larva / Q000378:metabolism / Q000648:ultrastructure; D009132:Muscles / Q000378:metabolism; D009154:Mutation / Q000235:genetics; D009469:Neuromuscular Junction / Q000378:metabolism / Q000648:ultrastructure; D018377:Neurotransmitter Agents / Q000378:metabolism; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction; D013569:Synapses / Q000378:metabolism* / Q000648:ultrastructure
chemical_list: D019485:Bone Morphogenetic Proteins; D029721:Drosophila Proteins; C509056:MAN1 protein, Drosophila; D018377:Neurotransmitter Agents; D009687:Nuclear Proteins
keywords: BMP signaling; Drosophila; Neuromuscular junction; Ultrastructure