title: An impedimetric biosensor for the diagnosis of renal cell carcinoma based on the interaction between 3-aminophenyl boronic acid and sialic acid.
abstract: Renal cell carcinoma (RCC) often expresses a high density of sialic acid-rich glycoproteins which helps these late-stage cancer cells to enter the blood stream or urine. Blood diagnosis is a complex and time-consuming process. In this study, we developed a facile and non-invasive electrochemical cytosensor for early detection of RCC in urine samples based on specific recognition by 3-aminophenyl boronic acid (APBA). Polypyrrole (PPy) and bovine serum albumin (BSA)-incorporated Ag submicron particles (Ag@BSA) were co-deposited on a gold electrode (GE) to take advantages of the excellent properties of these biomaterials, including good biocompatibility, low cytotoxicity and excellent electro-conductivity. To further increase the biosensor's sensitivity, APBA molecules were integrated to recognize sialic acid (SA) on the cell surface. Under optimal conditions, the impedimetric cytosensor exhibited a good linear relationship with the logarithm of the cell concentration from 17 to 1.7×106cellsmL-1, and the low detection limit was 6 cellsmL-1(S/N=3). Therefore, the electrochemical impedimetric biosensor offers a potential approach to bedside rapid detection of RCC in clinical applications.
authors.affiliation: School of Public Health and Management, Institute of Life Science, Chongqing Medical University, Chongqing 400016, PR China.
authors.forename: Lei
authors.initials: L
authors.lastname: Zhang
authors.affiliation: School of Public Health and Management, Institute of Life Science, Chongqing Medical University, Chongqing 400016, PR China.
authors.forename: Chao
authors.initials: C
authors.lastname: Yu
authors.affiliation: School of Public Health and Management, Institute of Life Science, Chongqing Medical University, Chongqing 400016, PR China.
authors.forename: Rufei
authors.initials: R
authors.lastname: Gao
authors.affiliation: School of Public Health and Management, Institute of Life Science, Chongqing Medical University, Chongqing 400016, PR China.
authors.forename: Yazhen
authors.initials: Y
authors.lastname: Niu
authors.affiliation: School of Public Health and Management, Institute of Life Science, Chongqing Medical University, Chongqing 400016, PR China.
authors.forename: Yuliang
authors.initials: Y
authors.lastname: Li
authors.affiliation: School of Public Health and Management, Institute of Life Science, Chongqing Medical University, Chongqing 400016, PR China.
authors.forename: Jun
authors.initials: J
authors.lastname: Chen
authors.affiliation: School of Public Health and Management, Institute of Life Science, Chongqing Medical University, Chongqing 400016, PR China. Electronic address: hejunlin_11@aliyun.com.
authors.forename: Junlin
authors.initials: J
authors.lastname: He
mesh_terms: D000818:Animals; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D001897:Boronic Acids / Q000737:chemistry*; D002292:Carcinoma, Renal Cell / Q000175:diagnosis / Q000652:urine*; D002417:Cattle; D002460:Cell Line; D045744:Cell Line, Tumor; D058266:Dielectric Spectroscopy / Q000295:instrumentation / Q000379:methods*; D004566:Electrodes; D004867:Equipment Design; D006801:Humans; D007680:Kidney Neoplasms / Q000175:diagnosis / Q000652:urine*; D019158:N-Acetylneuraminic Acid / Q000032:analysis / Q000652:urine*; D011108:Polymers / Q000737:chemistry*; D011758:Pyrroles / Q000737:chemistry*; D012680:Sensitivity and Specificity; D012710:Serum Albumin, Bovine / Q000737:chemistry; D012834:Silver / Q000737:chemistry
chemical_list: D001897:Boronic Acids; D011108:Polymers; D011758:Pyrroles; D012710:Serum Albumin, Bovine; C028592:3-aminobenzeneboronic acid; C067635:polypyrrole; D012834:Silver; D019158:N-Acetylneuraminic Acid
keywords: 3-aminophenyl boronic acid; Impedimetric cytosensor; PPy-Ag@BSA; Renal cell carcinoma; Sialic acid