title: Fluorescence-activated Cell Sorting for Purification of Plasmacytoid Dendritic Cells from the Mouse Bone Marrow.
abstract: Fluorescence-activated cell sorting (FACS) is a technique to purify specific cell populations based on phenotypes detected by flow cytometry. This method enables researchers to better understand the characteristics of a single cell population without the influence of other cells. Compared to other methods of cell enrichment, such as magnetic-activated cell sorting (MCS), FACS is more flexible and accurate for cell separation due to the ability of phenotype detection by flow cytometry. In addition, FACS is usually capable of separating multiple cell populations simultaneously, which improves the efficiency and diversity of experiments. Although FACS has some limitations, it has been broadly used to purify cells for functional studies in both in vitro and in vivo settings. Here we report a protocol using fluorescence-activated cell sorting to isolate a very rare population of immune cells, plasmacytoid dendritic cells (pDC), with high purity from the bone marrow of lupus-prone mice for in vitro functional studies of pDC.
authors.affiliation: Department of Biomedical Sciences and Pathobiology, Virginia-Maryland College of Veterinary Medicine, Virginia Polytechnic Institute and State University.
authors.forename: Xiaofeng
authors.initials: X
authors.lastname: Liao
authors.affiliation: Flow Cytometry Laboratory, Virginia-Maryland College of Veterinary Medicine, Virginia Polytechnic Institute and State University.
authors.forename: Melissa
authors.initials: M
authors.lastname: Makris
authors.affiliation: Department of Biomedical Sciences and Pathobiology, Virginia-Maryland College of Veterinary Medicine, Virginia Polytechnic Institute and State University; xinluo@vt.edu.
authors.forename: Xin M
authors.initials: XM
authors.lastname: Luo
mesh_terms: D000818:Animals; D001853:Bone Marrow; D001854:Bone Marrow Cells; D002469:Cell Separation; D003713:Dendritic Cells; D005434:Flow Cytometry; D008280:Magnetics; D051379:Mice