title: A highly sensitive "turn-on" fluorescent probe with an aggregation-induced emission characteristic for quantitative detection of γ-globulin.
abstract: As a novel "turn on" fluorescent probe, 4-((1Z,3Z)-1,4-bis(4-(methoxycarbonyl)phenyl)-4-(pyridin-4-yl)buta-1,3-dien-1-yl)-1-methylpyridin-1-ium hexafluorophosphate (TABD-Py-PF6) with an aggregation-induced emission characteristic was synthesized for in-situ quantitation of γ-globulins in the blood serum. It was shown that the TABD-Py-PF6probe was highly specific for γ-globulins and that other components in the blood serum, including serum albumins, fibrinogen, glucose, urea, and cholesterol, barely interfered with the molecular interactions between TABD-Py-PF6and γ-globulins. The high specificity of this probe enabled in-situ quantitative detection of γ-globulins without isolation of γ-globulins from the blood serum. The fluorescence intensity of TABD-Py-PF6was linearly correlated with the concentration of γ-globulins in the ranges of 7.89-300μg/mL. The detection limit of γ-globulins was determined to be 7.89μg/mL. The fluorescence response time of TABD-Py-PF6for detecting γ-globulins was very short (below 5s), allowing for real-time detection. The mechanism of the fluorescent turn-on behavior of the TABD-Py-PF6probe was investigated and electrostatic interactions between TABD-Py-PF6and γ-globulins were identified.
authors.affiliation: School of Life Science, Beijing Institute of Technology, 5 South Zhongguancun Street, Beijing 100081, PR China.
authors.forename: Pai
authors.initials: P
authors.lastname: Liu
authors.affiliation: School of Materials Science & Engineering, Beijing Institute of Technology, 5 South Zhongguancun Street, Beijing 100081, PR China.
authors.forename: Didi
authors.initials: D
authors.lastname: Chen
authors.affiliation: School of Materials Science & Engineering, Beijing Institute of Technology, 5 South Zhongguancun Street, Beijing 100081, PR China.
authors.forename: Yuanhang
authors.initials: Y
authors.lastname: Wang
authors.affiliation: School of Life Science, Beijing Institute of Technology, 5 South Zhongguancun Street, Beijing 100081, PR China.
authors.forename: Xiaoying
authors.initials: X
authors.lastname: Tang
authors.affiliation: School of Life Science, Beijing Institute of Technology, 5 South Zhongguancun Street, Beijing 100081, PR China.
authors.forename: Hanjun
authors.initials: H
authors.lastname: Li
authors.affiliation: School of Materials Science & Engineering, Beijing Institute of Technology, 5 South Zhongguancun Street, Beijing 100081, PR China; Beijing Key Laboratory of Construction Tailorable Advanced Functional Materials and Green Applications, Beijing Institute of Technology, Beijing 100081, PR China.
authors.forename: Jianbing
authors.initials: J
authors.lastname: Shi
authors.affiliation: School of Materials Science & Engineering, Beijing Institute of Technology, 5 South Zhongguancun Street, Beijing 100081, PR China; Beijing Key Laboratory of Construction Tailorable Advanced Functional Materials and Green Applications, Beijing Institute of Technology, Beijing 100081, PR China.
authors.forename: Bin
authors.initials: B
authors.lastname: Tong
authors.affiliation: School of Materials Science & Engineering, Beijing Institute of Technology, 5 South Zhongguancun Street, Beijing 100081, PR China; Beijing Key Laboratory of Construction Tailorable Advanced Functional Materials and Green Applications, Beijing Institute of Technology, Beijing 100081, PR China. Electronic address: chdongyp@bit.edu.cn.
authors.forename: Yuping
authors.initials: Y
authors.lastname: Dong
mesh_terms: D015374:Biosensing Techniques / Q000379:methods; D005456:Fluorescent Dyes / Q000737:chemistry*; D006801:Humans; D057230:Limit of Detection; D008958:Models, Molecular; D011726:Pyridinium Compounds / Q000737:chemistry*; D013050:Spectrometry, Fluorescence / Q000379:methods*; D005719:gamma-Globulins / Q000032:analysis*
chemical_list: D005456:Fluorescent Dyes; D011726:Pyridinium Compounds; D005719:gamma-Globulins
keywords: Aggregation-induced emission; Buta-1,3-diene derivative; In-situ detection; Pyridine salt; γ-Globulin