title: Capturing Excited States in the Fast-Intermediate Exchange Limit in Biological Systems Using1H NMR Spectroscopy.
abstract: Changes in molecular structure are essential for the function of biomolecules. Characterization of these structural fluctuations can illuminate alternative states and help in correlating structure to function. NMR relaxation dispersion (RD) is currently the only method for detecting these alternative, high-energy states. In this study, we present a versatile1H R1ρRD experiment that not only extends the exchange timescales at least three times beyond the rate limits of13C/15N R1ρand ten times for CPMG experiments, but also makes use of easily accessible probes, thus allowing a general description of biologically important excited states. This technique can be used to extract chemical shifts for the structural characterization of excited states and to elucidate complex excited states.
authors.affiliation: Department of Medical Biochemistry and Biophysics, Karolinska Institute, 10435, Stockholm, Sweden.
authors.forename: Emilie
authors.initials: E
authors.lastname: Steiner
authors.affiliation: Department of Medical Biochemistry and Biophysics, Karolinska Institute, 10435, Stockholm, Sweden.
authors.forename: Judith
authors.initials: J
authors.lastname: Schlagnitweit
authors.affiliation: Department of Physics, Chemistry and Biology, Linköping University, 58183, Linköping, Sweden.
authors.forename: Patrik
authors.initials: P
authors.lastname: Lundström
authors.affiliation: Department of Medical Biochemistry and Biophysics, Karolinska Institute, 10435, Stockholm, Sweden.
authors.forename: Katja
authors.identifier: 0000-0001-9470-0347
authors.initials: K
authors.lastname: Petzold
keywords: 1H NMR spectroscopy; biomolecular dynamics; excited states; nucleic acids; relaxation dispersion