title: Significance of diazotrophic plant growth-promoting Herbaspirillum sp. GW103 on phytoextraction of Pband Zn by Zea mays L.
abstract: Microbe-assisted phytoremediation has been considered a promising measure for the remediation of heavy metal-polluted soil. The aim of this study was to assess the effect of diazotrophic plant growth-promoting Herbaspirillum sp. GW103 on growth and lead (Pb) and zinc (Zn) accumulation in Zea mays L. The strain GW103 exhibited plant growth-promoting traits such as indole-3-acetic acid, siderophores, and 1-aminocyclopropane-1-carboxylic deaminase. Treatment of Z. mays L. plants with GW103 significantly increased 19, 31, and 52% of plant biomass and 10, 50, and 126% of chlorophyll a contents in Pb, Zn, and Pb + Zn-amended soils, respectively. Similarly, the strain GW103 significantly increased Pb and Zn accumulation in shoots and roots of Z. mays L., which were 77 and 25% in Pb-amended soil, 42 and 73% in Zn-amended soil, and 27 and 84% in Pb + Zn-amended soil. Furthermore, addition of GW103 increased 8, 12, and 7% of total protein content, catalase, and superoxide dismutase levels, respectively, in Z. mays L. plants. The results pointed out that isolate GW103 could potentially reduce the phytotoxicity of metals and increase Pb and Zn accumulation in Z. mays L. plant.
authors.affiliation: Division of Biotechnology, Advanced Institute of Environment and Bioscience, College of Environmental and Bioresource Sciences, Chonbuk National University, Iksan, 54596, South Korea.
authors.forename: Loganathan
authors.initials: L
authors.lastname: Praburaman
authors.affiliation: Department of Rehabilitation Medicine, School of Medicine, Chonbuk National University, Jeonju, Jeonbuk, 54896, South Korea.
authors.forename: Sung-Hee
authors.initials: SH
authors.lastname: Park
authors.affiliation: Division of Biotechnology, Advanced Institute of Environment and Bioscience, College of Environmental and Bioresource Sciences, Chonbuk National University, Iksan, 54596, South Korea.
authors.forename: Min
authors.initials: M
authors.lastname: Cho
authors.affiliation: Division of Biotechnology, Advanced Institute of Environment and Bioscience, College of Environmental and Bioresource Sciences, Chonbuk National University, Iksan, 54596, South Korea.
authors.forename: Kui-Jae
authors.initials: KJ
authors.lastname: Lee
authors.affiliation: Department of Horticulture, College of Agriculture and Life Sciences, Chonbuk National University, Jeonju, 54896, South Korea.
authors.forename: Jeong-Ae
authors.initials: JA
authors.lastname: Ko
authors.affiliation: Department of Forest Environment Science, College of Agriculture and Life Science, Chonbuk National University, Jeonju, 54896, South Korea.
authors.forename: Sang-Sub
authors.initials: SS
authors.lastname: Han
authors.affiliation: Department of Forest Environment Science, College of Agriculture and Life Science, Chonbuk National University, Jeonju, 54896, South Korea.
authors.forename: Sang-Hyun
authors.initials: SH
authors.lastname: Lee
authors.affiliation: Division of Biotechnology, Advanced Institute of Environment and Bioscience, College of Environmental and Bioresource Sciences, Chonbuk National University, Iksan, 54596, South Korea. kannan@jbnu.ac.kr.
authors.forename: Seralathan
authors.initials: S
authors.lastname: Kamala-Kannan
authors.affiliation: Division of Biotechnology, Advanced Institute of Environment and Bioscience, College of Environmental and Bioresource Sciences, Chonbuk National University, Iksan, 54596, South Korea. btoh@jbnu.ac.kr.|Plant Medical Research Center, College of Agricultural and Life Sciences, Chonbuk National University, Jeonju, Jeonbuk, 54896, South Korea. btoh@jbnu.ac.kr.
authors.forename: Byung-Taek
authors.initials: BT
authors.lastname: Oh
mesh_terms: D001673:Biodegradation, Environmental; D018533:Biomass; D002734:Chlorophyll / Q000031:analogs & derivatives / Q000378:metabolism; D000077194:Chlorophyll A; D042803:Herbaspirillum; D007210:Indoleacetic Acids / Q000378:metabolism; D007854:Lead; D063245:Plant Development; D018517:Plant Roots / Q000378:metabolism; D012987:Soil; D012989:Soil Pollutants / Q000032:analysis; D003313:Zea mays / Q000254:growth & development*; D015032:Zinc
chemical_list: D007210:Indoleacetic Acids; D012987:Soil; D012989:Soil Pollutants; D002734:Chlorophyll; C085815:chlorophyll a'; D007854:Lead; C030737:indoleacetic acid; D015032:Zinc; D000077194:Chlorophyll A
keywords: Antioxidant enzymes; Diazotrophic bacteria; Heavy metals; PGPR; Phytoremediation; Z. mays L.