title: Selenium uptake and assessment of the biochemical changes in Arthrospira (Spirulina) platensis biomass during the synthesis of selenium nanoparticles.
abstract: The process of selenium uptake by biomass of the cyanobacterium Arthrospira (Spirulina) platensis was investigated by neutron activation analysis at different selenium concentrations in solution and at different contact times. Experimental data showed good fit with the Freundlich adsorption isotherm model, with a regression coefficient value of 0.99. In terms of absorption dependence on time, the maximal selenium content was adsorbed in the first 5 min of interaction without significant further changes. It was also found that A. platensis biomass forms spherical selenium nanoparticles. Biochemical analysis was used to assess the changes in the main components of spirulina biomass (proteins, lipids, carbohydrates, and phycobilin) during nanoparticle formation.
authors.affiliation: a Joint Institute for Nuclear Research, Joliot-Curie Str., 6, 1419890 Dubna, Russia.|b Horia Hulubei National Institute for R&D in Physics and Nuclear Engineering, 30 Reactorului Str. MG-6, Bucharest - Magurele, Romania.|c The Institute of Chemistry of the Academy of Sciences of Moldova, 3, Academiei Str., 2028 Chisinau, Republic of Moldova.
authors.forename: I
authors.initials: I
authors.lastname: Zinicovscaia
authors.affiliation: d Institute of Microbiology and Biotechnology of the Academy of Science of Moldova, 1, Academiei Str., 2028 Chisinau, Republic of Moldova.
authors.forename: T
authors.initials: T
authors.lastname: Chiriac
authors.affiliation: d Institute of Microbiology and Biotechnology of the Academy of Science of Moldova, 1, Academiei Str., 2028 Chisinau, Republic of Moldova.
authors.forename: L
authors.initials: L
authors.lastname: Cepoi
authors.affiliation: d Institute of Microbiology and Biotechnology of the Academy of Science of Moldova, 1, Academiei Str., 2028 Chisinau, Republic of Moldova.
authors.forename: L
authors.initials: L
authors.lastname: Rudi
authors.affiliation: a Joint Institute for Nuclear Research, Joliot-Curie Str., 6, 1419890 Dubna, Russia.|e National Institute for R&D in Electrical Engineering ICPE-CA, Splaiul, Unirii, Nr. 313, District 3, 030138, Bucharest, Romania.
authors.forename: O
authors.initials: O
authors.lastname: Culicov
authors.affiliation: a Joint Institute for Nuclear Research, Joliot-Curie Str., 6, 1419890 Dubna, Russia.
authors.forename: M
authors.initials: M
authors.lastname: Frontasyeva
authors.affiliation: d Institute of Microbiology and Biotechnology of the Academy of Science of Moldova, 1, Academiei Str., 2028 Chisinau, Republic of Moldova.
authors.forename: V
authors.initials: V
authors.lastname: Rudic
mesh_terms: D001671:Biochemistry; D001692:Biological Transport; D018533:Biomass; D007700:Kinetics; D053758:Nanoparticles / Q000737:chemistry / Q000378:metabolism*; D012643:Selenium / Q000737:chemistry / Q000378:metabolism*; D053545:Spirulina / Q000737:chemistry / Q000254:growth & development* / Q000378:metabolism*
chemical_list: D012643:Selenium
keywords: Arthrospira (Spirulina) platensis; analyse par activation neutronique; biochemical changes; biosorption; changements biochimiques; microscopie électronique à balayage; nanoparticles; nanoparticules; neutron activation analysis; scanning electron microscopy; selenium; sélénium