title: Protective Effect of Deer Bone Oil on Cartilage Destruction in Rats with Monosodium Iodoacetate (MIA)-Induced Osteoarthritis.
abstract: The anti-osteoarthritic activity of the methanol fraction of deer bone oil extract (DBO-M) was evaluated in interleukin (IL)-1β-inflamed primary rabbit chondrocytes and in rats with monosodium iodoacetate (MIA)-induced osteoarthritis. The active compound in DBO-M was analyzed using a direct infusion liquid chromatography quadrupole (LCQ) ion-trap electrospray ionization (ESI)-mass spectrometer (MS). DBO-M significantly suppressed the IL-1β-induced sulfated-glycosaminoglycan (s-GAG) release from chondrocyte, and lowered mRNA levels of the collagen-degrading enzymes matrix metalloproteinase (MMP)-1 and MMP-3 in a dose-dependent manner. Upon treatment with high doses of DBO-M, the levels of IL-1β, tumor necrosis factor (TNF)-α, and IL-6 decreased by around 40, 70, and 50%, respectively, compared to the control in the serum of rats with MIA-induced osteoarthritis. Bone volume fraction (BV/TV) and trabecular thickness (Tb.Th) increased by over 40% in rats treated with DBO-M compared to the values reported for the MIA-treated control group, while trabecular separation (Tb.Sp) showed a significant decrease (ca. 38%), as confirmed through micro-computed tomography (CT) analysis of MIA-induced destruction of articular bones. Furthermore, direct infusion ESI-MS analysis showed that DBO-M contains gangliosides, which are glycosphingolipids with monosialic acid (GM3), as a major compound. Our results suggest that DBO-M effectively improves MIA-induced osteoarthritis by suppressing inflammatory responses, and that gangliosides could be one of the DBO-derived anti-inflammatory components.
authors.affiliation: Department of Food Science and Technology, Seoul Women's University.
authors.forename: Hyeon-Son
authors.initials: HS
authors.lastname: Choi
authors.forename: Suji
authors.initials: S
authors.lastname: Im
authors.forename: Je Won
authors.initials: JW
authors.lastname: Park
authors.forename: Hyung Joo
authors.initials: HJ
authors.lastname: Suh
mesh_terms: D000818:Animals; D001169:Arthritis, Experimental / Q000097:blood / Q000139:chemically induced / Q000000981:diagnostic imaging / Q000188:drug therapy*; D001842:Bone and Bones / Q000737:chemistry*; D002358:Cartilage, Articular / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D002784:Cholesterol / Q000097:blood; D019902:Chondrocytes / Q000187:drug effects; D045424:Complex Mixtures / Q000494:pharmacology / Q000627:therapeutic use*; D016207:Cytokines / Q000097:blood; D003670:Deer; D019807:Iodoacetic Acid; D008297:Male; D020781:Matrix Metalloproteinase 1 / Q000235:genetics; D019278:Matrix Metalloproteinase 3 / Q000235:genetics; D009821:Oils / Q000737:chemistry*; D010003:Osteoarthritis / Q000097:blood / Q000139:chemically induced / Q000000981:diagnostic imaging / Q000188:drug therapy*; D020011:Protective Agents / Q000494:pharmacology / Q000627:therapeutic use*; D012333:RNA, Messenger / Q000378:metabolism; D011817:Rabbits; D017208:Rats, Wistar; D014280:Triglycerides / Q000097:blood; D055114:X-Ray Microtomography
chemical_list: D045424:Complex Mixtures; D016207:Cytokines; D009821:Oils; D020011:Protective Agents; D012333:RNA, Messenger; D014280:Triglycerides; D002784:Cholesterol; D019278:Matrix Metalloproteinase 3; D020781:Matrix Metalloproteinase 1; D019807:Iodoacetic Acid