title: Identification of cardiovascular risk factors associated with bone marrow cell subsets in patients with STEMI: a biorepository evaluation from the CCTRN TIME and LateTIME clinical trials.
abstract: Autologous bone marrow mononuclear cell (BM-MNC) therapy for patients with ST-segment elevation myocardial infarction (STEMI) has produced inconsistent results, possibly due to BM-MNC product heterogeneity. Patient-specific cardiovascular risk factors (CRFs) may contribute to variations in BM-MNC composition. We sought to identify associations between BM-MNC subset frequencies and specific CRFs in STEMI patients. Bone marrow was collected from 191 STEMI patients enrolled in the CCTRN TIME and LateTIME trials. Relationships between BM-MNC subsets and CRFs were determined with multivariate analyses. An assessment of CRFs showed that hyperlipidemia and hypertension were associated with a higher frequency of CD11b+cells (P = 0.045 and P = 0.016, respectively). In addition, we found that females had lower frequencies of CD11b+(P = 0.018) and CD45+CD14+(P = 0.028) cells than males, age was inversely associated with the frequency of CD45+CD31+cells (P = 0.001), smoking was associated with a decreased frequency of CD45+CD31+cells (P = 0.013), glucose level was positively associated with the frequency of CD45+CD3+cells, and creatinine level (an indicator of renal function) was inversely associated with the frequency of CD45+CD3+cells (P = 0.015). In conclusion, the frequencies of monocytic, lymphocytic, and angiogenic BM-MNCs varied in relation to patients' CRFs. These phenotypic variations may affect cell therapy outcomes and might be an important consideration when selecting patients for and reviewing results from autologous cell therapy trials.
authors.affiliation: Texas Heart Institute, Houston, TX, USA.
authors.forename: Ariadna
authors.initials: A
authors.lastname: Contreras
authors.affiliation: Texas Heart Institute, Houston, TX, USA.
authors.forename: Aaron F
authors.initials: AF
authors.lastname: Orozco
authors.affiliation: Texas Heart Institute, Houston, TX, USA.
authors.forename: Micheline
authors.initials: M
authors.lastname: Resende
authors.affiliation: Houston Methodist DeBakey Heart and Vascular Center, Houston Methodist Research Institute, Houston, TX, USA.
authors.forename: Robert C
authors.initials: RC
authors.lastname: Schutt
authors.affiliation: Minneapolis Heart Institute Foundation, Abbott Northwestern Hospital, Minneapolis, MN, USA.
authors.forename: Jay H
authors.initials: JH
authors.lastname: Traverse
authors.affiliation: Cedars Sinai Institute, Los Angeles, CA, USA.
authors.forename: Timothy D
authors.initials: TD
authors.lastname: Henry
authors.affiliation: UT Health School of Public Health, Houston, TX, USA.
authors.forename: Dejian
authors.initials: D
authors.lastname: Lai
authors.affiliation: Houston Methodist DeBakey Heart and Vascular Center, Houston Methodist Research Institute, Houston, TX, USA.
authors.forename: John P
authors.initials: JP
authors.lastname: Cooke
authors.affiliation: University of Louisville, Louisville, KY, USA.
authors.forename: Roberto
authors.initials: R
authors.lastname: Bolli
authors.affiliation: UT Health School of Public Health, Houston, TX, USA.
authors.forename: Michelle L
authors.initials: ML
authors.lastname: Cohen
authors.affiliation: UT Health School of Public Health, Houston, TX, USA. lemmoye@msn.com.
authors.forename: Lem
authors.initials: L
authors.lastname: Moyé
authors.affiliation: College of Medicine, University of Florida, Gainesville, FL, USA.
authors.forename: Carl J
authors.initials: CJ
authors.lastname: Pepine
authors.affiliation: Stanford University School of Medicine, Stanford, CA, USA.
authors.forename: Phillip C
authors.initials: PC
authors.lastname: Yang
authors.affiliation: Texas Heart Institute, Houston, TX, USA.
authors.forename: Emerson C
authors.initials: EC
authors.lastname: Perin
authors.affiliation: Texas Heart Institute, Houston, TX, USA.
authors.forename: James T
authors.initials: JT
authors.lastname: Willerson
authors.affiliation: Texas Heart Institute, Houston, TX, USA.
authors.forename: Doris A
authors.initials: DA
authors.lastname: Taylor
mesh_terms: D000328:Adult; D000368:Aged; D001854:Bone Marrow Cells / Q000166:cytology*; D016026:Bone Marrow Transplantation; D002318:Cardiovascular Diseases; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000166:cytology; D008297:Male; D008875:Middle Aged; D009203:Myocardial Infarction / Q000503:physiopathology / Q000628:therapy; D010641:Phenotype; D012189:Retrospective Studies; D012307:Risk Factors
keywords: Autologous cell therapy; Bone marrow mononuclear cells; Cardiovascular risk factors; Ischemic heart disease; ST-segment elevation myocardial infarction