title: A Comparison of the Effects of Alpha and Medical-Grade Honey Ointments on Cutaneous Wound Healing in Rats.
abstract: Introduction. This study compared the healing efficacy and possible adverse effects of topical Alpha and medical-grade honey ointments on cutaneous wounds in rats.Methods. To conduct the study, 22 male Sprague-Dawley rats were randomly allocated into two equal groups: (1) rats with Alpha ointment applied to the wound surface area and (2) rats with medical-grade honey ointment applied to their wounds. The ointments were applied daily during the 21-day study period. Wound contraction was examined photographically with images taken on days 0, 7, and 21 after wounding. The healing process was histopathologically assessed using skin biopsies taken from the wound sites on days 7 and 21.Results. No statistically significant difference in mean wound surface area was observed between the two study groups. According to histopathological assessment, a significant reduction in the amount of collagen deposition (Pvalue: 0.007) and neovascularisation (Pvalue: 0.002) was seen in the Alpha-treated rats on day 21. No tissue necrosis occurred following the application of Alpha ointment.Conclusion. Daily topical usage of Alpha ointment on a skin wound can negatively affect the healing process by inhibiting neovascularization. Topical Alpha ointment can reduce the possibility of excessive scar formation by reducing collagen deposition.
authors.affiliation: Trauma Research Center, Shahid Rajaee (Emtiaz) Trauma Hospital, Shiraz University of Medical Sciences, Fars Province, Shiraz, Iran; Department of General Surgery, Shiraz University of Medical Sciences, Fars Province, Shiraz, Iran.
authors.forename: Shahram
authors.initials: S
authors.lastname: Paydar
authors.affiliation: Department of General Surgery, Shiraz University of Medical Sciences, Fars Province, Shiraz, Iran; Breast Diseases Research Center, Shiraz University of Medical Sciences, Fars Province, Shiraz, Iran.
authors.forename: Majid
authors.initials: M
authors.lastname: Akrami
authors.affiliation: Trauma Research Center, Shahid Rajaee (Emtiaz) Trauma Hospital, Shiraz University of Medical Sciences, Fars Province, Shiraz, Iran; Department of Pathology, School of Medicine, Shiraz University of Medical Sciences, Fars Province, Shiraz, Iran.
authors.forename: Amirreza
authors.initials: A
authors.lastname: Dehghanian
authors.affiliation: Trauma Research Center, Shahid Rajaee (Emtiaz) Trauma Hospital, Shiraz University of Medical Sciences, Fars Province, Shiraz, Iran.
authors.forename: Roshanak
authors.initials: R
authors.lastname: Alavi Moghadam
authors.affiliation: Trauma Research Center, Shahid Rajaee (Emtiaz) Trauma Hospital, Shiraz University of Medical Sciences, Fars Province, Shiraz, Iran.
authors.forename: Mohsen
authors.initials: M
authors.lastname: Heidarpour
authors.affiliation: Trauma Research Center, Shahid Rajaee (Emtiaz) Trauma Hospital, Shiraz University of Medical Sciences, Fars Province, Shiraz, Iran.
authors.forename: Amir
authors.initials: A
authors.lastname: Bahari Khoob
authors.affiliation: Student Research Committee, Shiraz University of Medical Sciences, Shiraz, Iran; Department of Internal Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.
authors.forename: Behnam
authors.identifier: 0000-0003-4547-0965
authors.initials: B
authors.lastname: Dalfardi