title: A novel plasmid carrying blaCTX-M-15identified in commensal Escherichia coli from healthy pregnant women in Ibadan, Nigeria.
abstract: The aim of this study was to investigate the molecular characteristics of commensal Escherichia coli producing extended-spectrum β-lactamases and showing fluoroquinolone resistance circulating in a healthy population in Ibadan, Nigeria. In total, 101 faecal samples from healthy pregnant women on the day of admission to hospital were collected and plated on eosin-methylene blue agar supplemented with cefotaxime. Genotyping demonstrated the presence of the blaCTX-M-15gene in all of the cefotaxime-resistant isolates (n=32), and there was circulation of prevalent clones. The aac(6')-Ib-cr, qnrS1, qepA1 and qnrB1 genes were identified in several strains. A novel plasmid supporting the spread of the blaCTX-M-15, blaTEM-1and qnrS1 genes was identified in these isolates by complete DNA sequencing.
authors.affiliation: Department of Infectious, Parasitic and Immune-Mediated Diseases, Istituto Superiore di Sanità, Viale Regina Elena 299, 00161 Rome, Italy.
authors.forename: Daniela
authors.initials: D
authors.lastname: Fortini
authors.affiliation: Department of Microbiology, University of Ibadan, Ibadan, Nigeria.
authors.forename: Kayode
authors.initials: K
authors.lastname: Fashae
authors.affiliation: Department of Infectious, Parasitic and Immune-Mediated Diseases, Istituto Superiore di Sanità, Viale Regina Elena 299, 00161 Rome, Italy.
authors.forename: Laura
authors.initials: L
authors.lastname: Villa
authors.affiliation: Department of Infectious, Parasitic and Immune-Mediated Diseases, Istituto Superiore di Sanità, Viale Regina Elena 299, 00161 Rome, Italy.
authors.forename: Claudia
authors.initials: C
authors.lastname: Feudi
authors.affiliation: Department of Infectious, Parasitic and Immune-Mediated Diseases, Istituto Superiore di Sanità, Viale Regina Elena 299, 00161 Rome, Italy.
authors.forename: Aurora
authors.initials: A
authors.lastname: García-Fernández
authors.affiliation: Department of Infectious, Parasitic and Immune-Mediated Diseases, Istituto Superiore di Sanità, Viale Regina Elena 299, 00161 Rome, Italy. Electronic address: alessandra.carattoli@iss.it.
authors.forename: Alessandra
authors.initials: A
authors.lastname: Carattoli
keywords: IncF plasmid; PMQR; Plasmid-mediated quinolone resistance