title: Selective Reaction Monitoring for Quantitation of Cellular Proteins.
abstract: Proteins and proteomes are dynamic and complex. The accurate identification and measurement of their properties such as abundance, location, and turnover are challenging tasks. Even though high-throughput proteomics has significantly evolved, the technique still lacks fully quantitative and reproducible qualities. A mass spectrometry-based targeted proteomic strategy called selective reaction monitoring (SRM) has emerged in recent years as an important multiplex platform to precisely quantify sets of proteins in multiple samples. This has several advantages in terms of sensitivity, reproducibility, and sample consumption compared to classical methods including those based on antibodies. Here, we present a detailed protocol for quantitation of panels of proteins from cell line extracts using the SRM targeted proteomics approach.
authors.affiliation: Department of Biochemistry and Immunology, RibeirãoPreto Medical School, University of São Paulo, RibeirãoPreto, SP, Brazil. vitor.faca@fmrp.usp.br.|Center for Cell Based Therapy - Hemotherapy Center of RibeirãoPreto, RibeirãoPreto Medical School, University of São Paulo, RibeirãoPreto, SP, Brazil. vitor.faca@fmrp.usp.br.
authors.forename: Vitor M
authors.initials: VM
authors.lastname: Faça
mesh_terms: D002460:Cell Line; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods; D020543:Proteome; D040901:Proteomics / Q000379:methods*; D012984:Software
chemical_list: D020543:Proteome
keywords: Mass spectrometry; Protein quantitation; Selective reaction monitoring; Targeted proteomics