title: Sphingomyelin Liposomes Containing Porphyrin-phospholipid for Irinotecan Chemophototherapy.
abstract: Porphyrin-phospholipid (PoP) liposomes can entrap anti-cancer agents and release them in response to near infrared (NIR) light. Doxorubicin, when remotely loaded via an ammonium sulfate gradient at a high drug-to-lipid ratio, formed elongated crystals that altered liposome morphology and could not be loaded into liposomes with higher PoP content. On the other hand, irinotecan could also be remotely loaded but did not form large crystals and did not induce liposome elongation. The loading, stability, and NIR light-triggered release of irinotecan in PoP liposomes was altered by the types of lipids used and the presence of PEGylation. Sphingomyelin, which has been explored previously for liposomal irinotecan, was found to produce liposomes with relatively improved serum stability and rapid NIR light-triggered drug release. PoP liposomes composed from sphingomyelin, cholesterol and 2 molar percent PoP rapidly released irinotecan in vivo in response to NIR irradiation as monitored by intravital microscopy and also induced effective tumor eradication in mice bearing MIA Paca-2 subcutaneous tumor xenografts.
authors.affiliation: Department of Biomedical Engineering, University at Buffalo, State University of New York, Buffalo, NY, 14260.
authors.forename: Kevin A
authors.initials: KA
authors.lastname: Carter
authors.affiliation: Department of Biomedical Engineering, University at Buffalo, State University of New York, Buffalo, NY, 14260.
authors.forename: Dandan
authors.initials: D
authors.lastname: Luo
authors.affiliation: Department of Biochemistry and Biomedical Sciences and M. G. DeGroote Institute for Infectious Diseases Research, McMaster University, Hamilton, ON L8S4L8, Canada.
authors.forename: Aida
authors.initials: A
authors.lastname: Razi
authors.affiliation: Department of Biomedical Engineering, University at Buffalo, State University of New York, Buffalo, NY, 14260.
authors.forename: Jumin
authors.initials: J
authors.lastname: Geng
authors.affiliation: Department of Biomedical Engineering, University at Buffalo, State University of New York, Buffalo, NY, 14260.
authors.forename: Shuai
authors.initials: S
authors.lastname: Shao
authors.affiliation: Department of Biochemistry and Biomedical Sciences and M. G. DeGroote Institute for Infectious Diseases Research, McMaster University, Hamilton, ON L8S4L8, Canada.
authors.forename: Joaquin
authors.initials: J
authors.lastname: Ortega
authors.affiliation: Department of Biomedical Engineering, University at Buffalo, State University of New York, Buffalo, NY, 14260.
authors.forename: Jonathan F
authors.initials: JF
authors.lastname: Lovell
mesh_terms: D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000008:administration & dosage* / Q000493:pharmacokinetics; D002166:Camptothecin / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000493:pharmacokinetics; D004195:Disease Models, Animal; D004337:Drug Carriers / Q000008:administration & dosage* / Q000737:chemistry; D064593:Heterografts; D007259:Infrared Rays; D000077146:Irinotecan; D008081:Liposomes / Q000008:administration & dosage*; D051379:Mice; D010190:Pancreatic Neoplasms / Q000473:pathology / Q000628:therapy; D010743:Phospholipids / Q000008:administration & dosage*; D011166:Porphyrins / Q000008:administration & dosage*; D013109:Sphingomyelins / Q000008:administration & dosage*; D016896:Treatment Outcome
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D004337:Drug Carriers; D008081:Liposomes; D010743:Phospholipids; D011166:Porphyrins; D013109:Sphingomyelins; D000077146:Irinotecan; D002166:Camptothecin
keywords: chemophototherapy; irinotecan; liposomes