title: Drug loaded nanoparticle coating on totally bioresorbable PLLA stents to prevent in-stent restenosis.
abstract: Biodegradable polymer poly (dl-lactide) (PDLLA) has been used as drug coating material for drug-eluting stents due to its excellent biocompatibility and sustained drug release ability. However, the uniform thin layer drug eluting coating on a stent not only inhibits the blood vessel's smooth muscle cell overgrowth but also delay the endotheliation process which is often associated with the occurrence of acute thrombosis. Therefore, in this study, we developed a novel coating method using PDLLA nanoparticles (NPs) as a coating to overcome this issue. The average 300 nm sized sirolimus-loaded PDLLA nanoparticles were prepared by a conventional emulsion solvent evaporation method. A low temperature plasma polymerization technology to graft hydrophilic polymers on to poly (l-lactide) stent was used to increase the surface coating efficiency of nanoparticles on the stent. Results showed that sirolimus-loaded nanoparticles can be successfully coated on to the stents with sustained drug release properties. In vitro cell culture study showed the drug loaded nanoparticle coating effectively inhibited the proliferation of smooth muscle cells while still allowed a faster proliferation of endothelial cells, suggesting that the new NP coated bioresorbable stents have the potential to reduce both the occurrence of in-stent restenosis and acute thrombosis. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 106B: 88-95, 2018.
authors.affiliation: Key Laboratory of Rubber-Plastics, Ministry of Education/Shandong Provincial Key Laboratory of Rubber-Plastic, Qingdao University of Science and Technology, 53 Zhengzhou Road, Qingdao, 266042, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Zhao
authors.affiliation: Key Laboratory of Rubber-Plastics, Ministry of Education/Shandong Provincial Key Laboratory of Rubber-Plastic, Qingdao University of Science and Technology, 53 Zhengzhou Road, Qingdao, 266042, China.
authors.forename: Zhichao
authors.initials: Z
authors.lastname: Mo
authors.affiliation: The Institute for Advanced Materials and Nano Biomedicine, Tongji University, Shanghai, 200092, China.
authors.forename: Fangfang
authors.initials: F
authors.lastname: Guo
authors.affiliation: The Institute for Advanced Materials and Nano Biomedicine, Tongji University, Shanghai, 200092, China.
authors.forename: Donglu
authors.initials: D
authors.lastname: Shi
authors.affiliation: National Institute for the Control of Pharmaceutical and Biological Products, Beijing, 100050, China.
authors.forename: Qian Qian
authors.initials: QQ
authors.lastname: Han
authors.affiliation: The Institute for Advanced Materials and Nano Biomedicine, Tongji University, Shanghai, 200092, China.|Beijing Advanced Medical Technologies, Co. Ltd., 5 Kaituo Road, Room A403, Beijing, 100085, China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Liu
mesh_terms: D020341:Absorbable Implants; D001807:Blood Vessel Prosthesis; D002478:Cells, Cultured; D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D003692:Delayed-Action Preparations; D042783:Endothelial Cells / Q000378:metabolism*; D006083:Graft Occlusion, Vascular / Q000517:prevention & control*; D006801:Humans; D009131:Muscle, Smooth, Vascular / Q000378:metabolism*; D032389:Myocytes, Smooth Muscle / Q000378:metabolism*; D053758:Nanoparticles / Q000737:chemistry*; D011091:Polyesters / Q000737:chemistry*; D015607:Stents
chemical_list: D020099:Coated Materials, Biocompatible; D003692:Delayed-Action Preparations; D011091:Polyesters; C033616:poly(lactide)
keywords: bioresorbable stents; human coronary artery endothelial cells (HCAECs); human coronary artery smooth muscle cells (HCASMCs); plasma polymerization; poly (lactide) nanoparticle; stenosis