title: Protective role of quercetin against hematotoxic and immunotoxic effects of furan in rats.
abstract: Furan (Fu) is a liver carcinogen produced during heating process of food and concerns a public health problem. The current study was undertaken to evaluate the protective role of quercetin (Que) on Fu-induced hematological, immunological, and histopathological alterations in rats. Fifty male Sprague Dawley rats were divided into five equal groups. Group I (Cont) received distilled water, group II (CO) received corn oil, group III (Que) received Que at 50 mg/kg BW, group IV (Fu) received Fu at 16 mg/kg BW, and group V received Que + Fu simultaneously; all groups gavaged daily for 30 days. Our results revealed that Fu administration significantly elevated RBCs, Hb, PCV, WBCs, lymphocytes, and granulocytes and reduced phagocytic percent (Ph%) and index (PhI). Fu decreased the serum total protein, albumin, globulin, IgM, IgG, and IL4, with a significant increase in the TNFα and 8-OHdG. Moreover, it decreased the GSH content and GST activity and increased the MDA levels in the splenic tissue. Histopathologically, Fu led to a moderate depletion in the lymphoid cells and weak immunostaining of CD20 antigen of few lymphocytes appeared in the spleen. Meanwhile, Que co-administration ameliorated the altered hematological parameters and improved the Ph% and PhI. It modulated the serum biochemical parameters and immunoglobulins. Moreover, it decreased lipid peroxidation and enhanced antioxidant status in the spleen. The results indicated that Que possesses antioxidant protective activity against Fu-induced oxidative damage and stimulates the immune function.
authors.affiliation: Department of Clinical Pathology, Faculty of Veterinary Medicine, Zagazig University, Zagazig, Sharkia, 44511, Egypt. rashaalam@ymail.com.
authors.forename: Rasha T
authors.initials: RT
authors.lastname: Alam
authors.affiliation: Department of Forensic Medicine and Toxicology, Faculty of Veterinary Medicine, Zagazig University, Zagazig, Sharkia, 44511, Egypt.
authors.forename: Ehsan H Abu
authors.initials: EH
authors.lastname: Zeid
authors.affiliation: Department of Forensic Medicine and Toxicology, Faculty of Veterinary Medicine, Zagazig University, Zagazig, Sharkia, 44511, Egypt.
authors.forename: Tamer S
authors.initials: TS
authors.lastname: Imam
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D005663:Furans / Q000633:toxicity*; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects; D011794:Quercetin / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D000975:Antioxidants; D005663:Furans; D011794:Quercetin; C039281:furan
keywords: Anti-oxidant; Furan; Immunotoxicity; Oxidative DNA damage; Quercetin; Spleen