title: Racemicystis persica sp. nov., a myxobacterium from soil.
abstract: A novel myxobacterium, strain MSr11462T, was isolated in 2015 from a soil sample collected form Kish Island beach, Persian Gulf, Iran. It displayed general myxobacterial features like Gram-negative staining, rod-shaped vegetative cells, gliding on solid surfaces, microbial lytic activity, fruiting-body-like aggregates and myxospore-like structures. The strain was mesophilic, aerobic and showed a chemoheterotrophic mode of nutrition. It was resistant to many antibiotics like gentamycin, polymyxin, fusidic acid and trimethoprim, and the key fatty acids of whole-cell hydrolysates were iso-C15 : 0, C16 : 0, iso-C17 : 0, C18 : 1, iso-C17 : 1 2-OH, C18 : 1 2-OH, iso-C15 : 0 OAG (O-alkylglycerol) and C16 : 1 OAG. The 16S rRNA gene sequence showed highest similarity (98.6 %) to Racemicystis crocea strain MSr9521T (GenBank accession no. KT591707). The phylogenetic analysis based on 16S rRNA gene sequences and matrix-assisted laser-desorption/ionization time-of-flight (MALDI-TOF) spectroscopy data supports a novel species of the family Polyangiaceae and the genus Racemicystis. DNA-DNA hybridization showed only about 50 % similarity between the novel strain and the phylogenetically closest species, Racemicystis. crocea MSr9521T. On the basis of a comprehensive taxonomic study, we propose a novel species, Racemicystis persica sp. nov., for strain MSr11462T (=DSM 103165T=NCCB 100606T).
authors.affiliation: Department of Microbiology, Faculty of Biological Sciences, Shahid Beheshti University, Tehran, Iran.
authors.forename: Azam
authors.initials: A
authors.lastname: Moradi
authors.affiliation: Department of Microbiology, Faculty of Biological Sciences, Shahid Beheshti University, Tehran, Iran.
authors.forename: Gholam Hosein
authors.initials: GH
authors.lastname: Ebrahimipour
authors.affiliation: Department of Microbial Drugs, Helmholtz Centre for Infection Research (HZI), Inhoffenstraße 7, D-38124 Braunschweig, Germany.
authors.forename: Kathrin I
authors.initials: KI
authors.lastname: Mohr
authors.affiliation: Department of Applied Microbiology, Justus Liebig University Gießen, Heinrich-Buff-Ring 26, D-35392 Gießen, Germany.
authors.forename: Peter
authors.initials: P
authors.lastname: Kämpfer
authors.affiliation: Department of Applied Microbiology, Justus Liebig University Gießen, Heinrich-Buff-Ring 26, D-35392 Gießen, Germany.
authors.forename: Stefanie P
authors.initials: SP
authors.lastname: Glaeser
authors.affiliation: Department of Pharmaceutical Biotechnology, Saarland University, Campus Building E8 1, 66123 Saarbrücken, Germany.|Department of Microbial Natural Products, Helmholtz Institute for Pharmaceutical Research Saarland (HIPS) - Helmholtz Centre for Infection Research (HZI), Saarland University, Campus Building E 8 1, 66123 Saarbrücken, Germany.
authors.forename: Fabienne
authors.initials: F
authors.lastname: Hennessen
authors.affiliation: Department of Pharmaceutical Biotechnology, Saarland University, Campus Building E8 1, 66123 Saarbrücken, Germany.|Department of Microbial Natural Products, Helmholtz Institute for Pharmaceutical Research Saarland (HIPS) - Helmholtz Centre for Infection Research (HZI), Saarland University, Campus Building E 8 1, 66123 Saarbrücken, Germany.
authors.forename: Katja
authors.initials: K
authors.lastname: Gemperlein
authors.affiliation: Department of Pharmaceutical Biotechnology, Saarland University, Campus Building E8 1, 66123 Saarbrücken, Germany.|Department of Microbial Natural Products, Helmholtz Institute for Pharmaceutical Research Saarland (HIPS) - Helmholtz Centre for Infection Research (HZI), Saarland University, Campus Building E 8 1, 66123 Saarbrücken, Germany.
authors.forename: Ram Prasad
authors.initials: RP
authors.lastname: Awal
authors.affiliation: Department of Microbial Drugs, Helmholtz Centre for Infection Research (HZI), Inhoffenstraße 7, D-38124 Braunschweig, Germany.
authors.forename: Corinna
authors.initials: C
authors.lastname: Wolf
authors.affiliation: Department of Pharmaceutical Biotechnology, Saarland University, Campus Building E8 1, 66123 Saarbrücken, Germany.|Department of Microbial Natural Products, Helmholtz Institute for Pharmaceutical Research Saarland (HIPS) - Helmholtz Centre for Infection Research (HZI), Saarland University, Campus Building E 8 1, 66123 Saarbrücken, Germany.
authors.forename: Rolf
authors.initials: R
authors.lastname: Müller
authors.affiliation: Department of Microbial Drugs, Helmholtz Centre for Infection Research (HZI), Inhoffenstraße 7, D-38124 Braunschweig, Germany.
authors.forename: Joachim
authors.initials: J
authors.lastname: Wink
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D007492:Iran; D062312:Islands; D009231:Myxococcales / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S