title: Facile synthesis of glucoamylase embedded metal-organic frameworks (glucoamylase-MOF) with enhanced stability.
abstract: The self-assembled glucoamylase metal-organic framework (glucoamylase-MOF) was synthesized by facile one-step method within 20min by simply mixing aqueous solution of 2-methylimidazole (160mM), glucoamylase (5mg/mL) and zinc acetate (40mM) at room temperature (28±2°C). The prepared glucoamylase-MOF was characterized by using FT-IR, confocal scanning laser microscopy, XRD and SEM. The robustness and thermal stability of glucoamylase embedded MOF was evaluated in terms of half-life (in the range of 60-80°C) which showed 6 folds increment as against free form. Further, in Michaelis-Menten kinetics studies, glucoamylase entrapped MOF exhibited higher Kmvalue and lower Vmaxvalue as compared to native enzyme. Moreover, the immobilized glucoamylase exhibited up to 57% of residual activity after six consecutive cycles of reuse, whereas it retained 91% of residual activity till 25days of storage. Finally, the conformational changes occurred after the encapsulation of glucoamylase in the interior of MOF, which was analyzed by using FT-IR data analysis tools.
authors.affiliation: Department of Chemical Engineering, Institute of Chemical Technology, Matunga (E) Mumbai 400019, India.
authors.forename: Shamraja S
authors.initials: SS
authors.lastname: Nadar
authors.affiliation: Department of Chemical Engineering, Institute of Chemical Technology, Matunga (E) Mumbai 400019, India. Electronic address: vk.rathod@ictmumbai.edu.in.
authors.forename: Virendra K
authors.initials: VK
authors.lastname: Rathod
mesh_terms: D060326:Chemistry Techniques, Synthetic; D004789:Enzyme Activation; D004795:Enzyme Stability; D004800:Enzymes, Immobilized / Q000737:chemistry / Q000378:metabolism; D005087:Glucan 1,4-alpha-Glucosidase / Q000737:chemistry* / Q000378:metabolism; D006207:Half-Life; D007700:Kinetics; D009942:Organometallic Compounds / Q000737:chemistry*; D017433:Protein Structure, Secondary; D013696:Temperature
chemical_list: D004800:Enzymes, Immobilized; D009942:Organometallic Compounds; D005087:Glucan 1,4-alpha-Glucosidase
keywords: Glucoamylase; Immobilization; Metal organic framework; Secondary structure; Self-assembled; Stability