title: Responses of the sea anemone, Exaiptasia pallida, to ocean acidification conditions and zinc or nickel exposure.
abstract: Ocean acidification, caused by increasing atmospheric carbon dioxide (CO2), is a growing concern in marine environments. Land-based sources of pollution, such as metals, have also been a noted problem; however, little research has addressed the combined exposure of both pollutants to coral reef organisms. In this study we examined tissue metal accumulation and physiological effects (activity of anti-oxidant enzymes, catalase and glutathione reductase) in the sea anemone, Exaiptasia pallida after exposure to increased CO2, as well as zinc (Zn) or nickel (Ni). After exposure to four concentrations (nominal values=control, 10, 50, 100μg/L) of Zn or Ni over 7days, both metals accumulated in the tissues of E. pallida in a concentration-dependent manner. Anemones exposed to elevated CO2(1000ppm) accumulated significant tissue burdens of Zn or Ni faster (by 48h) than those exposed to the same metal concentrations at ambient CO2. No differences were observed in catalase activity due to Zn exposure; however, 50μg/L Ni caused a significant increase in catalase activity at ambient CO2. No significant effect on catalase activity from CO2exposure alone was observed. Glutathione reductase activity was affected by increased Zn or Ni exposure and those effects were influenced by increased CO2. Results of this study provide insight into the toxic mechanisms and environmental implications of CO2and Zn or Ni exposure to the cnidarian E. pallida.
authors.affiliation: Department of Biology, Valdosta State University, Valdosta, GA, USA.
authors.forename: Christina G
authors.initials: CG
authors.lastname: Duckworth
authors.affiliation: Department of Biology, Valdosta State University, Valdosta, GA, USA.
authors.forename: Codie R
authors.initials: CR
authors.lastname: Picariello
authors.affiliation: Department of Biology, Valdosta State University, Valdosta, GA, USA.
authors.forename: Rachel K
authors.initials: RK
authors.lastname: Thomason
authors.affiliation: Department of Biology, Valdosta State University, Valdosta, GA, USA.
authors.forename: Krina S
authors.initials: KS
authors.lastname: Patel
authors.affiliation: Department of Chemistry, Jacksonville University, Jacksonville, FL, USA. Electronic address: gbielmy@ju.edu.
authors.forename: Gretchen K
authors.initials: GK
authors.lastname: Bielmyer-Fraser
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D015415:Biomarkers / Q000378:metabolism; D002245:Carbon Dioxide / Q000633:toxicity*; D002374:Catalase / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D005980:Glutathione Reductase / Q000378:metabolism; D009532:Nickel / Q000378:metabolism / Q000633:toxicity*; D009792:Oceans and Seas; D012615:Sea Anemones / Q000187:drug effects* / Q000378:metabolism; D018675:Toxicity Tests; D014874:Water Pollutants, Chemical / Q000633:toxicity*; D014876:Water Pollution / Q000009:adverse effects*; D015032:Zinc / Q000378:metabolism / Q000633:toxicity*
chemical_list: D000975:Antioxidants; D015415:Biomarkers; D014874:Water Pollutants, Chemical; D002245:Carbon Dioxide; D009532:Nickel; D002374:Catalase; D005980:Glutathione Reductase; D015032:Zinc
keywords: Accumulation; Acidification; Anemones; Nickel; Zinc