title: Method of Peptide Nucleic Acid (PNA)-Mediated Antisense Inhibition of Gene Expression in Campylobacter jejuni.
abstract: Peptide nucleic acid (PNA) is an oligonucleotide mimic that recognizes and binds to nucleic acids. The strong binding affinity of PNA to mRNA coupled with its high sequence specificity enable antisense PNA to selectively inhibit (i.e., knockdown) the protein synthesis of a target gene. This novel technology provides a powerful tool for Campylobacter studies because molecular techniques have been relatively less well-developed for this bacterium as compared to other pathogens, such as Escherichia coli and Salmonella. This chapter describes a protocol for PNA-mediated antisense inhibition of gene expression in Campylobacter jejuni.
authors.affiliation: School of Public Health, University of Alberta, 116th Street and 85 Avenue, Edmonton, AB, Canada, T6G 2 R3.
authors.forename: Euna
authors.initials: E
authors.lastname: Oh
authors.affiliation: School of Public Health, University of Alberta, 116th Street and 85 Avenue, Edmonton, AB, Canada, T6G 2 R3. bjeon@ualberta.ca.
authors.forename: Byeonghwa
authors.initials: B
authors.lastname: Jeon
mesh_terms: D000906:Antibodies / Q000737:chemistry; D001426:Bacterial Proteins / Q000037:antagonists & inhibitors / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D001681:Biological Assay; D015153:Blotting, Western; D016123:Campylobacter jejuni / Q000235:genetics* / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial; D016376:Oligonucleotides, Antisense / Q000138:chemical synthesis / Q000235:genetics* / Q000378:metabolism; D020135:Peptide Nucleic Acids / Q000138:chemical synthesis / Q000235:genetics* / Q000378:metabolism; D034622:RNA Interference; D012333:RNA, Messenger / Q000037:antagonists & inhibitors / Q000235:genetics* / Q000378:metabolism
chemical_list: D000906:Antibodies; D001426:Bacterial Proteins; D016376:Oligonucleotides, Antisense; D020135:Peptide Nucleic Acids; D012333:RNA, Messenger
keywords: Antisense; Campylobacter; Gene knockdown; Peptide nucleic acid (PNA)