title: Identification and Characterization of MicroRNAs in the Liver of Blunt Snout Bream (Megalobrama amblycephala) Infected by Aeromonas hydrophila.
abstract: MicroRNAs (miRNAs) are small RNA molecules that play key roles in regulation of various biological processes. In order to better understand the biological significance of miRNAs in the context ofAeromonas hydrophilainfection inMegalobrama amblycephala, small RNA libraries obtained from fish liver at 0 (non-infection), 4, and 24 h post infection (poi) were sequenced using Illumina deep sequencing technology. A total of 11,244,207, 9,212,958, and 7,939,157 clean reads were obtained from these three RNA libraries, respectively. Bioinformatics analysis identified 171 conserved miRNAs and 62 putative novel miRNAs. The existence of ten randomly selected novel miRNAs was validated by RT-PCR. Pairwise comparison suggested that 61 and 44 miRNAs were differentially expressed at 4 and 24 h poi, respectively. Furthermore, the expression profiles of nine randomly selected miRNAs were validated by qRT-PCR. MicroRNA target prediction, gene ontology (GO) annotation, and Kyoto Encylopedia of Genes and Genomes (KEGG) analysis indicated that a variety of biological pathways could be affected byA. hydrophilainfection. Additionally, transferrin (TF) and transferrin receptor (TFR) genes were confirmed to be direct targets of miR-375. These results will expand our knowledge of the role of miRNAs in the immune response ofM. amblycephalatoA. hydrophilainfection, and facilitate the development of effective strategies againstA. hydrophilainfection inM. amblycephala.
authors.affiliation: College of Fisheries, Key Lab of Freshwater Animal Breeding, Ministry of Agriculture, Huazhong Agricultural University, Wuhan 430070, China. cuilei.1987@webmail.hzau.edu.cn.
authors.forename: Lei
authors.initials: L
authors.lastname: Cui
authors.affiliation: Center for Bio-Pesticide Research, Hubei Academy of Agricultural Sciences, Wuhan 430064, China. hongtaohu@hbaas.com.
authors.forename: Hongtao
authors.initials: H
authors.lastname: Hu
authors.affiliation: College of Fisheries, Key Lab of Freshwater Animal Breeding, Ministry of Agriculture, Huazhong Agricultural University, Wuhan 430070, China. 18314787156@163.com.|Freshwater Aquaculture Collaborative Innovation Center of Hubei Province, Wuhan 430070, China. 18314787156@163.com.
authors.forename: Wei
authors.initials: W
authors.lastname: Wei
authors.affiliation: College of Fisheries, Key Lab of Freshwater Animal Breeding, Ministry of Agriculture, Huazhong Agricultural University, Wuhan 430070, China. wangwm@mail.hzau.edu.cn.
authors.forename: Weimin
authors.initials: W
authors.lastname: Wang
authors.affiliation: College of Fisheries, Key Lab of Freshwater Animal Breeding, Ministry of Agriculture, Huazhong Agricultural University, Wuhan 430070, China. liuhong59@mail.hzau.edu.cn.|Freshwater Aquaculture Collaborative Innovation Center of Hubei Province, Wuhan 430070, China. liuhong59@mail.hzau.edu.cn.
authors.forename: Hong
authors.initials: H
authors.lastname: Liu
mesh_terms: D016980:Aeromonas hydrophila / Q000472:pathogenicity*; D000818:Animals; D019295:Computational Biology; D003530:Cyprinidae / Q000378:metabolism* / Q000469:parasitology*; D029941:Fish Proteins / Q000235:genetics*; D020869:Gene Expression Profiling; D008099:Liver / Q000378:metabolism*; D035683:MicroRNAs / Q000235:genetics*; D011990:Receptors, Transferrin / Q000235:genetics; D014168:Transferrin / Q000235:genetics
chemical_list: D029941:Fish Proteins; D035683:MicroRNAs; D011990:Receptors, Transferrin; D014168:Transferrin
keywords: Aeromonas hydrophila; Megalobrama amblycephala; deep sequencing; immune response; miRNAs