title: iTRAQ-Based Quantitative Proteomic Comparison of Early- and Late-Passage Human Dermal Papilla Cell Secretome in Relation to Inducing Hair Follicle Regeneration.
abstract: Alopecia is an exceedingly prevalent problem that lacks effective therapy. Recently, research has focused on early-passage dermal papilla cells (DPCs), which have hair inducing activity both in vivo and in vitro. Our previous study indicated that factors secreted from early-passage DPCs contribute to hair follicle (HF) regeneration. To identify which factors are responsible for HF regeneration and why late-passage DPCs lose this potential, we collected 48-h-culture medium (CM) from both of passage 3 and 9 DPCs, and subcutaneously injected the DPC-CM into NU/NU mice. Passage 3 DPC-CM induced HF regeneration, based on the emergence of a white hair coat, but passage 9 DPC-CM did not. In order to identify the key factors responsible for hair induction, CM from passage 3 and 9 DPCs was analyzed by iTRAQ-based quantitative proteomic technology. We identified 1360 proteins, of which 213 proteins were differentially expressed between CM from early-passage vs. late-passage DPCs, including SDF1, MMP3, biglycan and LTBP1. Further analysis indicated that the differentially-expressed proteins regulated the Wnt, TGF-β and BMP signaling pathways, which directly and indirectly participate in HF morphogenesis and regeneration. Subsequently, we selected 19 proteins for further verification by multiple reaction monitoring (MRM) between the two types of CM. These results indicate DPC-secreted proteins play important roles in HF regeneration, with SDF1, MMP3, biglycan, and LTBP1 being potential key inductive factors secreted by dermal papilla cells in the regeneration of hair follicles.
authors.affiliation: Department of Histology and Embryology, Shantou University Medical College, Shantou, Guangdong, China.
authors.forename: Huan
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Department of Cardiology, First Affiliated Hospital, Shantou University Medical College, Shantou, Guangdong, China.
authors.forename: Ning-Xia
authors.initials: NX
authors.lastname: Zhu
authors.affiliation: Emergency Department, Second Affiliated Hospital, Shantou University Medical College, Shantou, Guangdong, China.
authors.forename: Keng
authors.initials: K
authors.lastname: Huang
authors.affiliation: Tissue Engineering Laboratory, First Affiliated Hospital, Shantou University Medical College, Shantou, Guangdong, China.
authors.forename: Bo-Zhi
authors.initials: BZ
authors.lastname: Cai
authors.affiliation: Department of Histology and Embryology, Shantou University Medical College, Shantou, Guangdong, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Zeng
authors.affiliation: Laboratory of Cancer Biology and Epigenetics, Department of Cell Biology and Genetics, Shantou University Medical College, Shantou, Guangdong, China.
authors.forename: Yan-Ming
authors.initials: YM
authors.lastname: Xu
authors.affiliation: Department of Histology and Embryology, Shantou University Medical College, Shantou, Guangdong, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Department of Histology and Embryology, Shantou University Medical College, Shantou, Guangdong, China.
authors.forename: Yan-Ping
authors.initials: YP
authors.lastname: Yuan
authors.affiliation: Department of Histology and Embryology, Shantou University Medical College, Shantou, Guangdong, China.
authors.forename: Chang-Min
authors.initials: CM
authors.lastname: Lin
mesh_terms: D000818:Animals; D058578:Biglycan / Q000378:metabolism; D054377:Chemokine CXCL12 / Q000378:metabolism; D019295:Computational Biology / Q000379:methods; D017077:Culture Media, Conditioned / Q000378:metabolism; D020405:Dermis / Q000166:cytology* / Q000378:metabolism*; D018859:Hair Follicle / Q000502:physiology*; D006801:Humans; D053779:Latent TGF-beta Binding Proteins / Q000378:metabolism; D019278:Matrix Metalloproteinase 3 / Q000378:metabolism; D051379:Mice; D020543:Proteome; D040901:Proteomics / Q000379:methods; D012038:Regeneration; D015203:Reproducibility of Results
chemical_list: D058578:Biglycan; D054377:Chemokine CXCL12; D017077:Culture Media, Conditioned; D053779:Latent TGF-beta Binding Proteins; D020543:Proteome; D019278:Matrix Metalloproteinase 3