title: Toward a new paradigm of DNA writing using a massively parallel sequencing platform and degenerate oligonucleotide.
abstract: All synthetic DNA materials require prior programming of the building blocks of the oligonucleotide sequences. The development of a programmable microarray platform provides cost-effective and time-efficient solutions in the field of data storage using DNA. However, the scalability of the synthesis is not on par with the accelerating sequencing capacity. Here, we report on a new paradigm of generating genetic material (writing) using a degenerate oligonucleotide and optomechanical retrieval method that leverages sequencing (reading) throughput to generate the desired number of oligonucleotides. As a proof of concept, we demonstrate the feasibility of our concept in digital information storage in DNA. In simulation, the ability to store data is expected to exponentially increase with increase in degenerate space. The present study highlights the major framework change in conventional DNA writing paradigm as a sequencer itself can become a potential source of making genetic materials.
authors.affiliation: Department of Chemistry, Yonsei University, Seoul 120-749, Republic of Korea.
authors.forename: Byungjin
authors.initials: B
authors.lastname: Hwang
authors.affiliation: Department of Chemistry, Yonsei University, Seoul 120-749, Republic of Korea.
authors.forename: Duhee
authors.initials: D
authors.lastname: Bang
mesh_terms: D019295:Computational Biology; D059014:High-Throughput Nucleotide Sequencing / Q000295:instrumentation / Q000379:methods*; D016247:Information Storage and Retrieval; D020411:Oligonucleotide Array Sequence Analysis / Q000295:instrumentation / Q000379:methods*; D017422:Sequence Analysis, DNA / Q000295:instrumentation / Q000379:methods*