title: A prospective cohort study to assess seroprevalence, incidence, knowledge, attitudes and practices, willingness to pay for vaccine and related risk factors in dengue in a high incidence setting.
abstract: Dengue is one of the most important vector-borne diseases in the world, causing significant morbidity and economic impact. In Colombia, dengue is a major public health problem. Departments of La Guajira, Cesar and Magdalena are dengue endemic areas. The objective of this research is to determine the seroprevalence and the incidence of dengue virus infection in the participating municipalities from these Departments, and also establish the association between individual and housing factors and vector indices with seroprevalence and incidence. We will also assess knowledge, attitudes and practices, and willingness-to-pay for dengue vaccine.
  
  
  
  A cohort study will be assembled with a clustered multistage sampling in 11 endemic municipalities. Approximately 1000 homes will be visited to enroll people older than one year who living in these areas, who will be followed for 1 year. Dengue virus infections will be evaluated using IgG indirect ELISA and IgM and IgG capture ELISA. Additionally, vector indices will be measured, and adult mosquitoes will be captured with aspirators. Ovitraps will be used for continuous estimation of vector density.
  
  
  
  This research will generate necessary knowledge to design and implement strategies with a multidimensional approach that reduce dengue morbidity and mortality in La Guajira and other departments from Colombian Caribbean.
authors.affiliation: Organización Latinoamericana para el Fomento de la Investigación en Salud, Bucaramanga, Santander, Colombia.|School of Medicine, Universidad de Santander, Bucaramanga, Santander, Colombia.
authors.forename: Ruth Aralí
authors.initials: RA
authors.lastname: Martínez-Vega
authors.affiliation: Organización Latinoamericana para el Fomento de la Investigación en Salud, Bucaramanga, Santander, Colombia.|Public Health and Infection Research Group, Faculty of Health Sciences, Universidad Tecnológica de Pereira, Pereira, Risaralda, Colombia.
authors.forename: Alfonso J
authors.initials: AJ
authors.lastname: Rodriguez-Morales
authors.affiliation: Organización Latinoamericana para el Fomento de la Investigación en Salud, Bucaramanga, Santander, Colombia.
authors.forename: Yalil Tomás
authors.initials: YT
authors.lastname: Bracho-Churio
authors.affiliation: Organización Latinoamericana para el Fomento de la Investigación en Salud, Bucaramanga, Santander, Colombia.
authors.forename: Mirley Enith
authors.initials: ME
authors.lastname: Castro-Salas
authors.affiliation: Organización Latinoamericana para el Fomento de la Investigación en Salud, Bucaramanga, Santander, Colombia.
authors.forename: Fredy
authors.initials: F
authors.lastname: Galvis-Ovallos
authors.affiliation: Organización Latinoamericana para el Fomento de la Investigación en Salud, Bucaramanga, Santander, Colombia.
authors.forename: Ronald Giovanny
authors.initials: RG
authors.lastname: Díaz-Quijano
authors.affiliation: Organización Latinoamericana para el Fomento de la Investigación en Salud, Bucaramanga, Santander, Colombia.
authors.forename: María Lucrecia
authors.initials: ML
authors.lastname: Luna-González
authors.affiliation: Grupo de Virología, Universidad El Bosque, Bogotá, Colombia.
authors.forename: Jaime E
authors.initials: JE
authors.lastname: Castellanos
authors.affiliation: Centro de Investigaciones sobre Enfermedades Infecciosas, Instituto Nacional de Salud Pública, Cuernavaca, Morelos, Mexico.
authors.forename: José
authors.initials: J
authors.lastname: Ramos-Castañeda
authors.affiliation: Department of Epidemiology, Universidade de São Paulo, São Paulo, São Paulo, Brazil. frediazq@msn.com.
authors.forename: Fredi Alexander
authors.initials: FA
authors.lastname: Diaz-Quijano
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002648:Child; D002675:Child, Preschool; D002985:Clinical Protocols; D003105:Colombia / Q000453:epidemiology; D003430:Cross-Sectional Studies; D003715:Dengue / Q000175:diagnosis / Q000191:economics / Q000453:epidemiology* / Q000517:prevention & control; D053059:Dengue Vaccines / Q000191:economics*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005500:Follow-Up Studies; D007722:Health Knowledge, Attitudes, Practice; D006301:Health Services Needs and Demand / Q000191:economics / Q000706:statistics & numerical data*; D006801:Humans; D015994:Incidence; D007223:Infant; D007231:Infant, Newborn; D008297:Male; D008875:Middle Aged; D011446:Prospective Studies; D012307:Risk Factors; D016036:Seroepidemiologic Studies; D055815:Young Adult
chemical_list: D053059:Dengue Vaccines
keywords: Colombia; Dengue; Incidence; Knowledge; Risk factors; Seroprevalence; Vaccines