title: In vitroantitumor activity of Latcripin-15 regulator of chromosome condensation 1 domain protein.
abstract: Cancer is one of the most significant health problems worldwide and thus the development of novel therapeutic agents with fewer side effects is required. The present study investigated thein vitroanticancer effects of a newly isolated fungal protein. In this study, Latcripin-15 (LP-15) regulator of chromosome condensation 1 (RCC1) domain protein, which is obtained from theLentinula edodesC91-3fungal strain, was identified, cloned, expressed, purified and re-folded to assess thein vitroantitumor activity of the protein. LP-15 RCC1 full-length cDNA was isolated fromLentinula edodesusing 3' and 5'-rapid amplification of cDNA ends and then cloned, expressed, purified and re-foldedin vitro. In addition, the effects of the isolated LP-15 RCC1 protein's functional domain on the viability and apoptosis of human lung cancer A549 cells were assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, transmission electron microscopy, flow cytometry and Hoechst 33258 staining. The LP-15 RCC1 functional domain protein was successfully expressed, purified and re-foldedin vitro. Treatment with the LP-15 RCC1 functional domain protein significantly reduced tumor cell viability and induced apoptosis in A549 cells. The results of the present study indicate that the LP-15 RCC1 functional domain requires further investigation as a novel therapeutic agent for cancer therapy.
authors.affiliation: Department of Medical Microbiology, Dalian Medical University, Dalian, Liaoning 116044, P.R. China.
authors.forename: Li
authors.initials: L
authors.lastname: Tian
authors.affiliation: Department of Medical Microbiology, Dalian Medical University, Dalian, Liaoning 116044, P.R. China.
authors.forename: Xiaoli
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Medical Microbiology, Dalian Medical University, Dalian, Liaoning 116044, P.R. China.
authors.forename: Xingyun
authors.initials: X
authors.lastname: Li
authors.affiliation: Department of Medical Microbiology, Dalian Medical University, Dalian, Liaoning 116044, P.R. China.
authors.forename: Ben
authors.initials: B
authors.lastname: Liu
authors.affiliation: Department of Medical Microbiology, Dalian Medical University, Dalian, Liaoning 116044, P.R. China.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.affiliation: Department of Medical Microbiology, Dalian Medical University, Dalian, Liaoning 116044, P.R. China.
authors.forename: Jing
authors.initials: J
authors.lastname: Cao
authors.affiliation: Department of Medical Microbiology, Dalian Medical University, Dalian, Liaoning 116044, P.R. China.
authors.forename: Anhong
authors.initials: A
authors.lastname: Ning
authors.affiliation: Department of Medical Microbiology, Dalian Medical University, Dalian, Liaoning 116044, P.R. China.
authors.forename: Min
authors.initials: M
authors.lastname: Huang
authors.affiliation: Department of Medical Microbiology, Dalian Medical University, Dalian, Liaoning 116044, P.R. China.
authors.forename: Mintao
authors.initials: M
authors.lastname: Zhong
keywords: Latcripin-15; Lentinula edodes; RCC1 domain; antitumor activity; apoptosis; prokaryotic expression