title: The enhancement of osteogenic capacity in a synthetic BMP-2 derived peptide coated mineralized collagen composite in the treatment of the mandibular defects.
abstract: The novel synthetic peptide P17-BMP-2 could promote cell attachment and enhance osteogenic capability. A composite, comprising nano-hydroxyapatite, collagen and poly(L-lactide) (nHAC/PLLA), was an efficient scaffold for carrier of P17-BMP-2. Our aim was to investigate whether nHAC/PLLA/P17-BMP-2 accelerates the osteogenesis as a reliable method for mandibular defect healing in this study. The repair capability was assessed by the gross observation, X-ray test and histological observation in four animal experiment groups at 2 week and 4 week after surgery: Group A (control), Group B (nHAC/PLLA treatment), Group C (nHAC/PLLA with 2 mg/g P17-BMP-2 treatment) and Group D (nHAC/PLLA with 10 mg/g P17-BMP-2 treatment). The Lane-Sandhu X-ray scores of the four groups were compared among four groups as well. The results showed that the composites containing the highest content of P17- BMP-2 performed best. Therefore, the nHAC/PLLA with P17-BMP-2 composite can accelerate the osteogenesis for mandibular defect healing and could be an ideal biological material as a bone graft material option for clinical applications.
authors.affiliation: Department of Traumatic Orthopaedics, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, Shandong Province, China.|Department of Orthopaedics, Liaocheng People's Hospital, Liaocheng Clinical School, Taishan Medical University, Liaocheng, Shandong Province, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Department of Traumatic Orthopaedics, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, Shandong Province, China.
authors.forename: Weidong
authors.initials: W
authors.lastname: Mu
authors.affiliation: Central Laboratory, Liaocheng People's Hospital, Medical School of Liaocheng, Zhong Yuan Academy of Biological Medicine, Liaocheng University, Shandong Province, China.
authors.forename: Shuangfeng
authors.initials: S
authors.lastname: Chen
authors.affiliation: Central Laboratory, Liaocheng People's Hospital, Medical School of Liaocheng, Zhong Yuan Academy of Biological Medicine, Liaocheng University, Shandong Province, China.
authors.forename: Dawei
authors.initials: D
authors.lastname: Yang
authors.affiliation: Central Laboratory, Liaocheng People's Hospital, Medical School of Liaocheng, Zhong Yuan Academy of Biological Medicine, Liaocheng University, Shandong Province, China.
authors.forename: Fei
authors.initials: F
authors.lastname: Xu
authors.affiliation: Central Laboratory, Liaocheng People's Hospital, Medical School of Liaocheng, Zhong Yuan Academy of Biological Medicine, Liaocheng University, Shandong Province, China.|Department of Clinical Chemistry and Haematology, University Medical Centre Utrecht, G03.550, Heidelberglaan 100, 3584 CX Utrecht, The Netherlands.
authors.forename: Yaping
authors.initials: Y
authors.lastname: Wu
mesh_terms: D000818:Animals; D055396:Bone Morphogenetic Protein 2 / Q000008:administration & dosage* / Q000627:therapeutic use; D018786:Bone Substitutes / Q000737:chemistry*; D003094:Collagen / Q000737:chemistry*; D017886:Durapatite / Q000737:chemistry*; D008334:Mandible / Q000187:drug effects* / Q000473:pathology / Q000503:physiopathology; D008338:Mandibular Injuries / Q000473:pathology / Q000503:physiopathology / Q000628:therapy*; D010012:Osteogenesis / Q000187:drug effects*; D010455:Peptides / Q000008:administration & dosage / Q000627:therapeutic use; D011091:Polyesters / Q000737:chemistry; D011817:Rabbits
chemical_list: D055396:Bone Morphogenetic Protein 2; D018786:Bone Substitutes; D010455:Peptides; D011091:Polyesters; C033616:poly(lactide); D003094:Collagen; D017886:Durapatite
keywords: Mineralized collagen composite; P17- BMP-2; mandibular defects; nHAC/PLLA