title: Functional marine metagenomic screening for anti-quorum sensing and anti-biofilm activity.
abstract: Quorum sensing (QS), a cell-to-cell communication process, entails the production of signaling molecules that enable synchronized gene expression in microbial communities to regulate myriad microbial functions, including biofilm formation. QS disruption may constitute an innovative approach to the design of novel antifouling and anti-biofilm agents. To identify novel quorum sensing inhibitors (QSI), 2,500 environmental bacterial artificial chromosomes (BAC) from uncultured marine planktonic bacteria were screened for QSI activity using soft agar overlaid with wild type Chromobacterium violaceum as an indicator. Of the BAC library clones, 7% showed high QSI activity (>40%) against the indicator bacterium, suggesting that QSI is common in the marine environment. The most active compound, eluted from BAC clone 14-A5, disrupted QS signaling pathways and reduced biofilm formation in both Pseudomonas aeruginosa and Acinetobacter baumannii. The mass spectra of the active BAC clone (14-A5) that had been visualized by thin layer chromatography was dominated by a m/z peak of 362.1.
authors.affiliation: a Avram and Stella Goldstein-Goren Department of Biotechnology Engineering, Faculty of Engineering Sciences , Ben-Gurion University of the Negev , Beer-Sheva , Israel.
authors.forename: Karin
authors.initials: K
authors.lastname: Yaniv
authors.affiliation: a Avram and Stella Goldstein-Goren Department of Biotechnology Engineering, Faculty of Engineering Sciences , Ben-Gurion University of the Negev , Beer-Sheva , Israel.
authors.forename: Karina
authors.initials: K
authors.lastname: Golberg
authors.affiliation: a Avram and Stella Goldstein-Goren Department of Biotechnology Engineering, Faculty of Engineering Sciences , Ben-Gurion University of the Negev , Beer-Sheva , Israel.
authors.forename: Esti
authors.initials: E
authors.lastname: Kramarsky-Winter
authors.affiliation: a Avram and Stella Goldstein-Goren Department of Biotechnology Engineering, Faculty of Engineering Sciences , Ben-Gurion University of the Negev , Beer-Sheva , Israel.|b The Ilse Katz Center for Meso and Nanoscale Science and Technology , Ben-Gurion University of the Negev , Beer-Sheva , Israel.
authors.forename: Robert
authors.initials: R
authors.lastname: Marks
authors.affiliation: c Faculty of Biology , Technion-Israel Institute of Technology , Haifa , Israel.
authors.forename: Alina
authors.initials: A
authors.lastname: Pushkarev
authors.affiliation: c Faculty of Biology , Technion-Israel Institute of Technology , Haifa , Israel.
authors.forename: Oded
authors.initials: O
authors.lastname: Béjà
authors.affiliation: a Avram and Stella Goldstein-Goren Department of Biotechnology Engineering, Faculty of Engineering Sciences , Ben-Gurion University of the Negev , Beer-Sheva , Israel.|b The Ilse Katz Center for Meso and Nanoscale Science and Technology , Ben-Gurion University of the Negev , Beer-Sheva , Israel.
authors.forename: Ariel
authors.initials: A
authors.lastname: Kushmaro
mesh_terms: D040981:Acinetobacter baumannii / Q000235:genetics / Q000502:physiology*; D018441:Biofilms / Q000254:growth & development*; D002855:Chromatography, Thin Layer; D002861:Chromobacterium / Q000235:genetics / Q000502:physiology; D022202:Chromosomes, Artificial, Bacterial; D056186:Metagenomics / Q000379:methods*; D011550:Pseudomonas aeruginosa / Q000235:genetics / Q000502:physiology*; D053038:Quorum Sensing / Q000187:drug effects / Q000235:genetics
keywords: Functional metagenomics; biofilm; marine environment; quorum sensing