title: Magnetic iron oxide modified pyropheophorbide-a fluorescence nanoparticles as photosensitizers for photodynamic therapy against ovarian cancer (SKOV-3) cells.
abstract: Magnetic iron oxide modified pyropheophorbide-a fluorescence nanoparticles, Fe3O4@SiO2@APTES@PPa (FSAP), were designed as magnetically targeted photodynamic antineoplastic agents and prepared through continuous covalent chemical modification on the surface of Fe3O4nanoparticles. The properties of the intermediates and the final product were comprehensively characterized by transmission electron microscopy, powder X-ray diffraction analysis, Fourier transform infrared spectroscopy, vibrating sample magnetometry, zeta potential measurement, ultraviolet-visible absorption spectroscopy, fluorescence emission spectroscopy, and thermogravimetric analysis. In this work, we demonstrated the in vitro photodynamic therapy (PDT) of FSAP against ovarian cancer (SKOV-3) cells, which indicated that FSAP could be taken up successfully and showed low dark toxicity without irradiation, but remarkable phototoxicity after irradiation. Meanwhile, FSAP had showed good biocompatibility and low dark toxicity against normal cells in the biological experiments on mouse normal fibroblast cell lines (L929 cells). In addition, in the photochemical process of FSAP mediated photodynamic therapy, the Type-II photo-oxygenation process (generated singlet oxygen) played an important role in the induction of cell damage.
authors.affiliation: Key Laboratory of Molecular Cytogenetics and Genetic Breeding of Heilongjiang Province. Harbin, College of Life Science and Technology, Harbin Normal University, Harbin, 150025, China. guoprof@163.com.
authors.forename: Guanghui
authors.initials: G
authors.lastname: Tan
authors.affiliation: College of Chemistry & Chemical Engineering, Harbin Normal University, Harbin, 150025, China. jyxprof@163.com quprof@163.com.
authors.forename: Wenting
authors.initials: W
authors.lastname: Li
authors.affiliation: College of Chemistry & Chemical Engineering, Harbin Normal University, Harbin, 150025, China. jyxprof@163.com quprof@163.com.
authors.forename: Jianjun
authors.initials: J
authors.lastname: Cheng
authors.affiliation: College of Chemistry & Chemical Engineering, Harbin Normal University, Harbin, 150025, China. jyxprof@163.com quprof@163.com.
authors.forename: Zhiqiang
authors.initials: Z
authors.lastname: Wang
authors.affiliation: College of Chemistry & Chemical Engineering, Harbin Normal University, Harbin, 150025, China. jyxprof@163.com quprof@163.com.
authors.forename: Shuquan
authors.initials: S
authors.lastname: Wei
authors.affiliation: College of Chemistry & Chemical Engineering, Harbin Normal University, Harbin, 150025, China. jyxprof@163.com quprof@163.com.
authors.forename: Yingxue
authors.initials: Y
authors.lastname: Jin
authors.affiliation: Key Laboratory of Molecular Cytogenetics and Genetic Breeding of Heilongjiang Province. Harbin, College of Life Science and Technology, Harbin Normal University, Harbin, 150025, China. guoprof@163.com and College of Chemistry & Chemical Engineering, Harbin Normal University, Harbin, 150025, China. jyxprof@163.com quprof@163.com.
authors.forename: Changhong
authors.initials: C
authors.lastname: Guo
authors.affiliation: Key Laboratory of Molecular Cytogenetics and Genetic Breeding of Heilongjiang Province. Harbin, College of Life Science and Technology, Harbin Normal University, Harbin, 150025, China. guoprof@163.com and College of Chemistry & Chemical Engineering, Harbin Normal University, Harbin, 150025, China. jyxprof@163.com quprof@163.com.
authors.forename: Fengyu
authors.initials: F
authors.lastname: Qu
mesh_terms: D000818:Animals; D002460:Cell Line; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects*; D005260:Female; D005290:Ferric Compounds / Q000737:chemistry*; D006801:Humans; D008280:Magnetics; D051379:Mice; D053758:Nanoparticles / Q000737:chemistry*; D010051:Ovarian Neoplasms; D010778:Photochemotherapy; D017319:Photosensitizing Agents / Q000737:chemistry* / Q000494:pharmacology; D012822:Silicon Dioxide
chemical_list: D005290:Ferric Compounds; D017319:Photosensitizing Agents; C000499:ferric oxide; D012822:Silicon Dioxide