title: Association between Leishmania infantum DNA in the hair of dogs and their infectiousness to Lutzomyia longipalpis.
abstract: Diagnosis of infection with Leishmania infantum by DNA detection in the hair has been recently demonstrated in dogs and wild animals. Our objective was to investigate if polymerase chain reaction (PCR) in hair might be used to identify infectious dogs. Thus, we assessed the infectiousness to Lutzomyia longipalpis by xenodiagnosis in comparison with the detection of L. infantum DNA by PCR in the hair, and with serology for anti-Leishmania IgG by ELISA in 15 positive dogs for L. infantum infection. Eight healthy dogs were included as negative controls. Among the 15 infected dogs, 13 were found positive in the ELISA (87%), 12 were PCR positive in the hair (80%), and 10 were positive in xenodiagnosis (67%). Positivity in the hair was associated with positivity in spleen (p=0.0003), seropositivity for antibodies (p=0.0006) and parasite transmission to L. longipalpis (p=0.0028). Considering the benefits to animal welfare and feasibility of hair sampling method, studies in larger and more diverse populations of naturally infected dogs from endemic areas should be conducted to evaluate the sensitivity, specificity, and predictive values of PCR using hair as a possible biomarker of infectiousness in dogs.
authors.affiliation: Laboratory of Veterinary Infectious Diseases, Hospital of Veterinary Medicine (HOSPMEV), Federal University of Bahia (UFBA), Av. Adhemar de Barros 500, Salvador, BA, CEP: 40170-110, Brazil.
authors.forename: Rafaela
authors.initials: R
authors.lastname: de Sousa Gonçalves
authors.affiliation: Laboratory of Veterinary Infectious Diseases, Hospital of Veterinary Medicine (HOSPMEV), Federal University of Bahia (UFBA), Av. Adhemar de Barros 500, Salvador, BA, CEP: 40170-110, Brazil; Department of Preventive Veterinary Medicine and Animal Production of the School of Veterinary Medicine and Zootechny, UFBA, Salvador, BA, Brazil.
authors.forename: Carlos R
authors.initials: CR
authors.lastname: Franke
authors.affiliation: Laboratory of Veterinary Infectious Diseases, Hospital of Veterinary Medicine (HOSPMEV), Federal University of Bahia (UFBA), Av. Adhemar de Barros 500, Salvador, BA, CEP: 40170-110, Brazil.
authors.forename: Jairo T
authors.initials: JT
authors.lastname: Magalhães-Junior
authors.affiliation: Laboratory of Cellular and Molecular Biology, HOSPMEV, UFBA, Salvador, BA, Brazil.
authors.forename: Bárbara M P S
authors.initials: BM
authors.lastname: Souza
authors.affiliation: Laboratory of Pathology and Bio-Intervention, Gonçalo Moniz Research Center, Oswaldo Cruz Foundation-FIOCRUZ/BA, R. Waldemar Falcão 121, Salvador, CEP: 40296-710, BA, Brazil.
authors.forename: Manuela S
authors.initials: MS
authors.lastname: Solcà
authors.affiliation: Laboratory of Veterinary Infectious Diseases, Hospital of Veterinary Medicine (HOSPMEV), Federal University of Bahia (UFBA), Av. Adhemar de Barros 500, Salvador, BA, CEP: 40170-110, Brazil; Department of Veterinary Anatomy, Pathology and Clinics of the School of Veterinary Medicine and Zootechny, UFBA, Salvador, BA, Brazil.
authors.forename: Daniela F
authors.initials: DF
authors.lastname: Larangeira
authors.affiliation: Laboratory of Veterinary Infectious Diseases, Hospital of Veterinary Medicine (HOSPMEV), Federal University of Bahia (UFBA), Av. Adhemar de Barros 500, Salvador, BA, CEP: 40170-110, Brazil; Department of Veterinary Anatomy, Pathology and Clinics of the School of Veterinary Medicine and Zootechny, UFBA, Salvador, BA, Brazil. Electronic address: barrouin@ufba.br.
authors.forename: Stella Maria
authors.initials: SM
authors.lastname: Barrouin-Melo
mesh_terms: D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood; D016054:DNA, Protozoan / Q000032:analysis*; D004283:Dog Diseases / Q000175:diagnosis*; D004285:Dogs; D006197:Hair / Q000737:chemistry* / Q000469:parasitology; D007303:Insect Vectors / Q000469:parasitology*; D018314:Leishmania infantum / Q000235:genetics / Q000502:physiology*; D007898:Leishmaniasis, Visceral / Q000175:diagnosis / Q000469:parasitology / Q000662:veterinary*; D011576:Psychodidae / Q000469:parasitology*
chemical_list: D000913:Antibodies, Protozoan; D016054:DNA, Protozoan
keywords: Biomarker; Hair shaft; Leishmaniasis; PCR; Sand flies; Xenodiagnosis