title: Advances in downstream processing of biologics - Spectroscopy: An emerging process analytical technology.
abstract: Process analytical technologies (PAT) for the manufacturing of biologics have drawn increased interest in the last decade. Besides being encouraged by the Food and Drug Administration's (FDA's) PAT initiative, PAT promises to improve process understanding, reduce overall production costs and help to implement continuous manufacturing. This article focuses on spectroscopic tools for PAT in downstream processing (DSP). Recent advances and future perspectives will be reviewed. In order to exploit the full potential of gathered data, chemometric tools are widely used for the evaluation of complex spectroscopic information. Thus, an introduction into the field will be given.
authors.affiliation: Institute of Engineering in Life Sciences, Section IV: Biomolecular Separation Engineering, Karlsruhe Institute of Technology, Engler-Bunte Ring 3, 76131 Karlsruhe, Germany.
authors.forename: Matthias
authors.initials: M
authors.lastname: Rüdt
authors.affiliation: Institute of Engineering in Life Sciences, Section IV: Biomolecular Separation Engineering, Karlsruhe Institute of Technology, Engler-Bunte Ring 3, 76131 Karlsruhe, Germany.
authors.forename: Till
authors.initials: T
authors.lastname: Briskot
authors.affiliation: Institute of Engineering in Life Sciences, Section IV: Biomolecular Separation Engineering, Karlsruhe Institute of Technology, Engler-Bunte Ring 3, 76131 Karlsruhe, Germany. Electronic address: juergen.hubbuch@kit.edu.
authors.forename: Jürgen
authors.initials: J
authors.lastname: Hubbuch
mesh_terms: D001688:Biological Products / Q000032:analysis* / Q000592:standards; D011786:Quality Control; D013057:Spectrum Analysis / Q000379:methods*; D013678:Technology, Pharmaceutical / Q000379:methods*
chemical_list: D001688:Biological Products
keywords: Biologics; Chemometrics; Downstream processing; Process analytical technology; Spectroscopy