title: Concurrent zero-dimensional and one-dimensional biomineralization of gold from a solution of Au3+and bovine serum albumin.
abstract: A technique was developed for preparing a novel material that consists of gold nanoparticles trapped within a fiber of unfolded proteins. These fibers are made in an aqueous solution that contains HAuCl4and the protein, bovine serum albumin (BSA). By changing the ratio of gold to BSA in solution, two different types of outcomes are observed. At lower gold to BSA ratios (30-120), a purple solution results after heating the mixture at 80 °C for 4 h. At higher gold to BSA ratios (130-170), a clear solution containing purple fibers results after heating the mixture at 80 °C for 4 h. UV-Vis spectroscopy and light scattering techniques show growth in nanocolloid size as gold to BSA ratio rises above 100. Data indicate that, for the higher gold to BSA ratios, the gold is sequestered within the solid material. The material mass, visible by eye, appears to be an aggregation of smaller individual fibers. Scanning electron microscopy and transmission electron microscopy indicate that these fibers are primarily one-dimensional aggregates, which can display some branching, and can be as narrow as 400 nm in size. The likely mechanism for the synthesis of the novel material is discussed.
authors.affiliation: Department of Chemistry, American University, 4400 Massachusetts Avenue, NW, Washington, DC 20016, USA.
authors.forename: Matthew R
authors.initials: MR
authors.lastname: Hartings
authors.affiliation: Department of Chemistry, American University, 4400 Massachusetts Avenue, NW, Washington, DC 20016, USA.
authors.forename: Noah
authors.initials: N
authors.lastname: Benjamin
authors.affiliation: Department of Chemistry, American University, 4400 Massachusetts Avenue, NW, Washington, DC 20016, USA.
authors.forename: Floriene
authors.initials: F
authors.lastname: Briere
authors.affiliation: Department of Chemistry, American University, 4400 Massachusetts Avenue, NW, Washington, DC 20016, USA.
authors.forename: Maria
authors.initials: M
authors.lastname: Briscione
authors.affiliation: Department of Chemistry, American University, 4400 Massachusetts Avenue, NW, Washington, DC 20016, USA.
authors.forename: Omar
authors.initials: O
authors.lastname: Choudary
authors.affiliation: Department of Chemistry, American University, 4400 Massachusetts Avenue, NW, Washington, DC 20016, USA.
authors.forename: Tamra L
authors.initials: TL
authors.lastname: Fisher
authors.affiliation: Department of Chemistry, American University, 4400 Massachusetts Avenue, NW, Washington, DC 20016, USA.
authors.forename: Laura
authors.initials: L
authors.lastname: Flynn
authors.affiliation: Department of Chemistry, American University, 4400 Massachusetts Avenue, NW, Washington, DC 20016, USA.
authors.forename: Elizabeth
authors.initials: E
authors.lastname: Ghias
authors.affiliation: Department of Chemistry, American University, 4400 Massachusetts Avenue, NW, Washington, DC 20016, USA.
authors.forename: Michaela
authors.initials: M
authors.lastname: Harper
authors.affiliation: Department of Chemistry, American University, 4400 Massachusetts Avenue, NW, Washington, DC 20016, USA.
authors.forename: Nader
authors.initials: N
authors.lastname: Khamis
authors.affiliation: Department of Chemistry, American University, 4400 Massachusetts Avenue, NW, Washington, DC 20016, USA.
authors.forename: Catherine
authors.initials: C
authors.lastname: Koenigsknecht
authors.affiliation: Department of Chemistry, American University, 4400 Massachusetts Avenue, NW, Washington, DC 20016, USA.
authors.forename: Klare
authors.initials: K
authors.lastname: Lazor
authors.affiliation: Department of Chemistry, American University, 4400 Massachusetts Avenue, NW, Washington, DC 20016, USA.
authors.forename: Steven
authors.initials: S
authors.lastname: Moss
authors.affiliation: Department of Chemistry, American University, 4400 Massachusetts Avenue, NW, Washington, DC 20016, USA.
authors.forename: Elaine
authors.initials: E
authors.lastname: Robbins
authors.affiliation: Department of Chemistry, American University, 4400 Massachusetts Avenue, NW, Washington, DC 20016, USA.
authors.forename: Susan
authors.initials: S
authors.lastname: Schultz
authors.affiliation: Department of Chemistry, American University, 4400 Massachusetts Avenue, NW, Washington, DC 20016, USA.
authors.forename: Samiye
authors.initials: S
authors.lastname: Yaman
authors.affiliation: Department of Chemistry, US Naval Academy, Anapolis, MD 21402, USA.
authors.forename: Luke M
authors.initials: LM
authors.lastname: Haverhals
authors.affiliation: Department of Chemistry, US Naval Academy, Anapolis, MD 21402, USA.
authors.forename: Paul C
authors.initials: PC
authors.lastname: Trulove
authors.affiliation: Directorate of Math, Information, and Life Sciences, US Air Force Office of Scientific Research, Arlington, VA 22203, USA.
authors.forename: Hugh C
authors.initials: HC
authors.lastname: De Long
authors.affiliation: Department of Chemistry, American University, 4400 Massachusetts Avenue, NW, Washington, DC 20016, USA.
authors.forename: Abigail E
authors.initials: AE
authors.lastname: Miller
authors.affiliation: Department of Chemistry, American University, 4400 Massachusetts Avenue, NW, Washington, DC 20016, USA.
authors.forename: Douglas M
authors.initials: DM
authors.lastname: Fox
keywords: biomineralization; gold nanoparticle; peptide-templated nanoparticle; protein aggregation; protein-templated nanoparticle