title: Stable Upconversion Nanohybrid Particles for Specific Prostate Cancer Cell Immunodetection.
abstract: Prostate cancer is one of the male killing diseases and early detection of prostate cancer is the key for better treatment and lower cost. However, the number of prostate cancer cells is low at the early stage, so it is very challenging to detect. In this study, we successfully designed and developed upconversion immune-nanohybrids (UINBs) with sustainable stability in a physiological environment, stable optical properties and highly specific targeting capability for early-stage prostate cancer cell detection. The developed UINBs were characterized by transmission electron microscopy (TEM), X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR), dynamic light scattering (DLS) and luminescence spectroscopy. The targeting function of the biotinylated antibody nanohybrids were confirmed by immunofluorescence assay and western blot analysis. The UINB system is able to specifically detect prostate cancer cells with stable and background-free luminescent signals for highly sensitive prostate cancer cell detection. This work demonstrates a versatile strategy to develop UCNPs based sustainably stable UINBs for sensitive diseased cell detection.
authors.affiliation: International Joint Center for Biomedical Innovation, Henan University, Kaifeng, Henan, 457001, China.|Department of Chemistry and Biomolecular Sciences, Macquarie University, Sydney, NSW 2109, Australia.
authors.forename: Yu
authors.initials: Y
authors.lastname: Shi
authors.affiliation: International Joint Center for Biomedical Innovation, Henan University, Kaifeng, Henan, 457001, China.|Faculty of Medicine &Health Sciences, Macquarie University, Sydney, NSW, 2109, Australia.|ARC Centre of Excellence for Nanoscale BioPhotonics, Macquarie University, Sydney, NSW, 2109, Australia.
authors.forename: Bingyang
authors.initials: B
authors.lastname: Shi
authors.affiliation: Department of Chemistry and Biomolecular Sciences, Macquarie University, Sydney, NSW 2109, Australia.|ARC Centre of Excellence for Nanoscale BioPhotonics, Macquarie University, Sydney, NSW, 2109, Australia.
authors.forename: Arun V Everest
authors.initials: AV
authors.lastname: Dass
authors.affiliation: ARC Centre of Excellence for Nanoscale BioPhotonics, Macquarie University, Sydney, NSW, 2109, Australia.
authors.forename: Yiqing
authors.initials: Y
authors.lastname: Lu
authors.affiliation: Department of Chemistry and Biomolecular Sciences, Macquarie University, Sydney, NSW 2109, Australia.
authors.forename: Nima
authors.initials: N
authors.lastname: Sayyadi
authors.affiliation: Department of Chemistry and Biomolecular Sciences, Macquarie University, Sydney, NSW 2109, Australia.
authors.forename: Liisa
authors.initials: L
authors.lastname: Kautto
authors.affiliation: Department of Chemistry and Biomolecular Sciences, Macquarie University, Sydney, NSW 2109, Australia.
authors.forename: Robert D
authors.initials: RD
authors.lastname: Willows
authors.affiliation: International Joint Center for Biomedical Innovation, Henan University, Kaifeng, Henan, 457001, China.|Faculty of Medicine &Health Sciences, Macquarie University, Sydney, NSW, 2109, Australia.
authors.forename: Roger
authors.initials: R
authors.lastname: Chung
authors.affiliation: ARC Centre of Excellence for Nanoscale BioPhotonics, Macquarie University, Sydney, NSW, 2109, Australia.
authors.forename: James
authors.initials: J
authors.lastname: Piper
authors.affiliation: Department of Chemistry and Biomolecular Sciences, Macquarie University, Sydney, NSW 2109, Australia.
authors.forename: Helena
authors.initials: H
authors.lastname: Nevalainen
authors.affiliation: Minomic International Ltd, Macquarie Park, Sydney, NSW, 2109, Australia.
authors.forename: Bradley
authors.initials: B
authors.lastname: Walsh
authors.affiliation: ARC Centre of Excellence for Nanoscale BioPhotonics, Macquarie University, Sydney, NSW, 2109, Australia.|Institute for Biomedical Materials and Devices, Faculty of Science, University of Technology, Sydney, NSW, 2007, Australia.
authors.forename: Dayong
authors.initials: D
authors.lastname: Jin
authors.affiliation: Department of Chemistry and Biomolecular Sciences, Macquarie University, Sydney, NSW 2109, Australia.|ARC Centre of Excellence for Nanoscale BioPhotonics, Macquarie University, Sydney, NSW, 2109, Australia.
authors.forename: Nicolle H
authors.initials: NH
authors.lastname: Packer
mesh_terms: D058948:Cell Tracking / Q000379:methods*; D055088:Early Detection of Cancer; D006801:Humans; D008163:Luminescent Measurements; D008297:Male; D046529:Microscopy, Electron, Transmission; D053758:Nanoparticles / Q000737:chemistry* / Q000627:therapeutic use; D011467:Prostate / Q000000981:diagnostic imaging / Q000473:pathology*; D011471:Prostatic Neoplasms / Q000175:diagnosis* / Q000000981:diagnostic imaging / Q000503:physiopathology; D017550:Spectroscopy, Fourier Transform Infrared