title: Transcriptional analysis of genes encoding β-glucosidase of Schizophyllum commune KUC9397 under optimal conditions.
abstract: The present study was conducted to determine the gene responsible for beta-glucosidase (BGL) production and to generate a full-length complementary DNA (cDNA) of one of the putative BGL genes, which showed a significant expression level when Schizophyllum commune KUC9397 was grown in optimized medium. The relative expression levels of seven genes encoding BGL of S. commune KUC9397 were determined with real-time quantitative reverse transcription PCR in cellulose-containing optimized medium (OM) compared to glucose-containing basal medium (BM). The most abundant transcript was bgl3a in OM. The transcript number of the bgl3a increased more than 57.60-fold when S. commune KUC9397 was grown on cellulose-containing OM compared to that on glucose-containing BM. The bgl3a was identified, and a deduced amino acid sequence of bgl3a shared homology (97%) with GH3 BGL of S. commune H4-8. This is the first report showing the transcription levels of genes encoding BGL and identification of full-length cDNA of glycoside hydrolase 3 (GH3) BGL from S. commune. Furthermore, this study is one of the steps for consolidated bioprocessing of lignocellulosic biomass to bioethanol.
authors.affiliation: Division of Environmental Science and Ecological Engineering, College of Life Sciences and Biotechnology, Korea University, 5-1 Anam-dong, Seongbuk-gu, Seoul, 136-701, South Korea.
authors.forename: Young Min
authors.initials: YM
authors.lastname: Lee
authors.affiliation: Division of Environmental Science and Ecological Engineering, College of Life Sciences and Biotechnology, Korea University, 5-1 Anam-dong, Seongbuk-gu, Seoul, 136-701, South Korea.
authors.forename: Hanbyul
authors.initials: H
authors.lastname: Lee
authors.affiliation: Division of Environmental Science and Ecological Engineering, College of Life Sciences and Biotechnology, Korea University, 5-1 Anam-dong, Seongbuk-gu, Seoul, 136-701, South Korea.
authors.forename: Young Mok
authors.initials: YM
authors.lastname: Heo
authors.affiliation: Division of Environmental Science and Ecological Engineering, College of Life Sciences and Biotechnology, Korea University, 5-1 Anam-dong, Seongbuk-gu, Seoul, 136-701, South Korea.
authors.forename: Hwanhwi
authors.initials: H
authors.lastname: Lee
authors.affiliation: Division of Environmental Science and Ecological Engineering, College of Life Sciences and Biotechnology, Korea University, 5-1 Anam-dong, Seongbuk-gu, Seoul, 136-701, South Korea.
authors.forename: Joo-Hyun
authors.initials: JH
authors.lastname: Hong
authors.affiliation: Division of Environmental Science and Ecological Engineering, College of Life Sciences and Biotechnology, Korea University, 5-1 Anam-dong, Seongbuk-gu, Seoul, 136-701, South Korea. jae-jinkim@korea.ac.kr.
authors.forename: Jae-Jin
authors.initials: JJ
authors.lastname: Kim
mesh_terms: D000595:Amino Acid Sequence; D003470:Culture Media / Q000737:chemistry; D020869:Gene Expression Profiling; D008969:Molecular Sequence Data; D060888:Real-Time Polymerase Chain Reaction; D012567:Schizophyllum / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development*; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D015533:Transcriptional Activation; D015854:Up-Regulation; D001617:beta-Glucosidase / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D003470:Culture Media; D001617:beta-Glucosidase