title: An immuno-wall microdevice exhibits rapid and sensitive detection of IDH1-R132H mutation specific to grade II and III gliomas.
abstract: World Health Organization grade II and III gliomas most frequently occur in the central nervous system (CNS) in adults. Gliomas are not circumscribed; tumor edges are irregular and consist of tumor cells, normal brain tissue, and hyperplastic reactive glial cells. Therefore, the tumors are not fully resectable, resulting in recurrence, malignant progression, and eventual death. Approximately 69-80% of grade II and III gliomas harbor mutations in the isocitrate dehydrogenase 1 gene (IDH1), of which 83-90% are found to be theIDH1-R132Hmutation. Detection of theIDH1-R132Hmutation should help in the differential diagnosis of grade II and III gliomas from other types of CNS tumors and help determine the boundary between the tumor and normal brain tissue. In this study, we established a highly sensitive antibody-based device, referred to as the immuno-wall, to detect theIDH1-R132Hmutation in gliomas. The immuno-wall causes an immunoreaction in microchannels fabricated using a photo-polymerizing polymer. This microdevice enables the analysis of theIDH1status with a small sample within 15 min with substantially high sensitivity. Our results suggested that 10% content of theIDH1-R132Hmutation in a sample of 0.33 μl volume, with 500 ng protein, or from 500 cells is theoretically sufficient for the analysis. The immuno-wall device will enable the rapid and highly sensitive detection of theIDH1-R132Hmutation in routine clinical practice.
authors.affiliation: Department of Neurosurgery, Nagoya University Graduate School of Medicine, Nagoya, Japan; Department of Neurosurgery, Mie University Graduate School of Medicine, Tsu, Japan.
authors.forename: Akane
authors.initials: A
authors.lastname: Yamamichi
authors.affiliation: Department of Applied Chemistry, Graduate School of Engineering, Nagoya University , Nagoya , Japan.
authors.forename: Toshihiro
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authors.lastname: Kasama
authors.affiliation: Department of Neurosurgery, Nagoya University Graduate School of Medicine , Nagoya , Japan.
authors.forename: Fumiharu
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authors.lastname: Ohka
authors.affiliation: Department of Neurosurgery, Nagoya University Graduate School of Medicine , Nagoya , Japan.
authors.forename: Hiromichi
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authors.lastname: Suzuki
authors.affiliation: Department of Neurosurgery, Nagoya University Graduate School of Medicine , Nagoya , Japan.
authors.forename: Akira
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authors.lastname: Kato
authors.affiliation: Department of Neurosurgery, Nagoya University Graduate School of Medicine , Nagoya , Japan.
authors.forename: Kazuya
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authors.lastname: Motomura
authors.affiliation: Department of Neurosurgery, Nagoya University Graduate School of Medicine , Nagoya , Japan.
authors.forename: Masaki
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authors.lastname: Hirano
authors.affiliation: Department of Neurosurgery, Nagoya University Graduate School of Medicine , Nagoya , Japan.
authors.forename: Melissa
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authors.lastname: Ranjit
authors.affiliation: Department of Neurosurgery, Nagoya University Graduate School of Medicine , Nagoya , Japan.
authors.forename: Lushun
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authors.affiliation: Department of Neurosurgery, Nagoya University Graduate School of Medicine , Nagoya , Japan.
authors.forename: Michihiro
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authors.lastname: Kurimoto
authors.affiliation: Department of Neurosurgery, Nagoya University Graduate School of Medicine , Nagoya , Japan.
authors.forename: Goro
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authors.affiliation: Department of Neurosurgery, Nagoya University Graduate School of Medicine , Nagoya , Japan.
authors.forename: Kosuke
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authors.lastname: Aoki
authors.affiliation: Department of Applied Chemistry, Graduate School of Engineering, Nagoya University , Nagoya , Japan.
authors.forename: Noritada
authors.initials: N
authors.lastname: Kaji
authors.affiliation: Division of Applied Chemistry, Hokkaido University , Sapporo , Japan.
authors.forename: Manabu
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authors.lastname: Tokeshi
authors.affiliation: Department of Neurosurgery, Mie University Graduate School of Medicine , Tsu , Japan.
authors.forename: Toshio
authors.initials: T
authors.lastname: Matsubara
authors.affiliation: Division of Cancer Biology, Nagoya University Graduate School of Medicine , Nagoya , Japan.
authors.forename: Takeshi
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authors.lastname: Senga
authors.affiliation: Department of Regional Innovation, Tohoku University Graduate School of Medicine , Sendai , Japan.
authors.forename: Mika K
authors.initials: MK
authors.lastname: Kaneko
authors.affiliation: Department of Neurosurgery, Mie University Graduate School of Medicine , Tsu , Japan.
authors.forename: Hidenori
authors.initials: H
authors.lastname: Suzuki
authors.affiliation: Department of Neurosurgery, Nagoya University Graduate School of Medicine , Nagoya , Japan.
authors.forename: Masahito
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authors.lastname: Hara
authors.affiliation: Department of Neurosurgery, Nagoya University Graduate School of Medicine , Nagoya , Japan.
authors.forename: Toshihiko
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authors.lastname: Wakabayashi
authors.affiliation: Department of Applied Chemistry, Graduate School of Engineering, Nagoya University , Nagoya , Japan.
authors.forename: Yoshinobu
authors.initials: Y
authors.lastname: Baba
authors.affiliation: Department of Regional Innovation, Tohoku University Graduate School of Medicine , Sendai , Japan.
authors.forename: Yukinari
authors.initials: Y
authors.lastname: Kato
authors.affiliation: Department of Neurosurgery, Nagoya University Graduate School of Medicine , Nagoya , Japan.
authors.forename: Atsushi
authors.initials: A
authors.lastname: Natsume
keywords: 30 Bio-inspired and biomedical materials; 404 Materials informatics / Genomics; Glioma; immuno-wall microdevice; isocitrate dehydrogenase 1 mutation; precision medicine; rapid diagnosis