title: Effectiveness and biocompatibility of a novel biological adhesive application for repair of meniscal tear on the avascular zone.
abstract: We have investigated the effectiveness and safety of a newly developed biological adhesive for repair of meniscal tear. The adhesive was composed of disuccinimidyl tartrate (DST) as a crosslinker and human serum albumin (HSA) as a hardener. To determine adequate concentration, bonding strength was measured using a tensiometer 5 min after applying the adhesive on the avascular zone tear of porcine meniscus; it was compared with the strengths of commercially available cyanoacrylate-based and fibrin-based adhesives.In vivoexamination was performed using Japanese white rabbits, creating longitudinal tears on the avascular zone of meniscus and applying DST-HSA adhesive. Three months after operation the rabbits were sacrificed and tension test and histological evaluation were performed. Bonding strength was measured in three porcine meniscus groups: (i) only suturing, (ii) suturing after applying the adhesive on surface and (iii) suturing using an adhesive-soaked suture. The optimum concentrations were 0.1 mmol of DST and 42 w/v% of HAS. Bonding strength was greatest with cyanoacrylate-based adhesive, followed by DST-HSA adhesive, and fibrin-based adhesive. No inflammation was observed in the synovium or surrounding tissues 3 months after using the DST-HSA adhesive. Bonding strength was greatest with DST-HSA adhesive-soaked suturing group (77 ± 6 N), followed by suturing only group (61 ± 5 N) and surface adhesive application group (60 ± 8 N). The newly developed DST-HSA adhesive is considered safe and may be effective in enforcement of bonding of avascular zone tear of the meniscus.
authors.affiliation: Department of Orthopaedic Surgery, School of Medicine, Shimane University, 89-1 Enya, Izumo, Shimane, 693-8501, Japan.
authors.forename: Takahito
authors.initials: T
authors.lastname: Inoue
authors.affiliation: Biomaterial, Nano-Bio field, International Center for Materials Nanoarchitectonics (MANA), National Institute for Materials Science, 1-1 Namiki, Tsukuba, Ibaraki, 305-0044, Japan.
authors.forename: Tetsushi
authors.initials: T
authors.lastname: Taguchi
authors.affiliation: Department of Orthopaedic Surgery, School of Medicine, Shimane University, 89-1 Enya, Izumo, Shimane, 693-8501, Japan.
authors.forename: Shinji
authors.initials: S
authors.lastname: Imade
authors.affiliation: Department of Orthopaedic Surgery, School of Medicine, Shimane University, 89-1 Enya, Izumo, Shimane, 693-8501, Japan.
authors.forename: Nobuyuki
authors.initials: N
authors.lastname: Kumahashi
authors.affiliation: Department of Orthopaedic Surgery, School of Medicine, Shimane University, 89-1 Enya, Izumo, Shimane, 693-8501, Japan.
authors.forename: Yuji
authors.initials: Y
authors.lastname: Uchio
keywords: biological adhesive; disuccinimidyl tartrate; human serum albumin; meniscal tear