title: Anti-tumor effects of Abnormal Savda Munziq on the transplanted cervical cancer (U27) mouse model.
abstract: BACKGROUND
  Abnormal Savda Munziq (ASMq), a traditional uyghur medicine, has shown anti-tumour properties in vitro. it was showed that total flavonoids of ASMq could inhibit the proliferation and enhance the antioxidant ability of human cervix cancer HeLa cell. This study attempts to confirm these effects on the transplanted cervical cancer (U27) mouse model in vivo.
  
  
  METHODS
  Forty eight Kunming mice were randomly divided in to six groups: normal control group (Control group), U27 tumor model group (Model group), cyclophosphamide administration group (CTX group),low-dose ASMq group (ASMq.L group), medium-dose ASMq group (ASMq.M group), and high-dose ASMq group (ASMq.H group). The five groups except normal control group transplanted with cervical cancer (U27) cells. We observed mice tumor inhibition rate and conducted the histopathological analysisUsing the western blot assay, the expression of TGF-β1 and TNF-α protein in transplanted cervical cancer U27 tumor tissue were detected.
  
  
  RESULTS
  The tumor inhibition rates of CTX group, ASMq.L group, ASMq.M group, and ASMq.H group were 72.21, 31.27, 60.53 and 51.94% respectively, has obvious antitumor effect. ASMq significantly promote the spleen tlymphocyte proliferation of transplanted cervical cancer U27 mice. Invasive growth and diffusion rate in tumor tissue were accelerate in the transplanted cervical cancer U27 model group. Tumor tissue necrosis of tumor cells are smaller in the medium, high dosage group. Compared with the U27 model group, the expression levels of TGF-β1 protein and TNF-α protein expression exhibited statistically significant decreased in the mice tumor tissues in the CTX administration group and the ASMq administration group.
  
  
  CONCLUSIONS
  ASMq has some antitumor effects on U27 model mice in vivo, The effects are achieved not only by improving the immune function of U27 model mice, but also by inhibiting the expression levels of TGF-β1 protein while promoting the expression levels of TNF-α protein.
authors.affiliation: Department of pharmaceutical analysis, Xinjiang Medical University, Urumqi, 830011, China.
authors.forename: Zuhragul
authors.initials: Z
authors.lastname: Omarniyaz
authors.affiliation: Uyghur Medical College, Xinjiang Medical University, Urumqi, 830011, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Yu
authors.affiliation: Department of pharmaceutical analysis, Xinjiang Medical University, Urumqi, 830011, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Yang
authors.affiliation: Department of pharmaceutical analysis, Xinjiang Medical University, Urumqi, 830011, China.
authors.forename: Lianlian
authors.initials: L
authors.lastname: Shan
authors.affiliation: Department of pharmaceutical analysis, Xinjiang Medical University, Urumqi, 830011, China.
authors.forename: Weiwei
authors.initials: W
authors.lastname: Miao
authors.affiliation: Department of pharmaceutical analysis, Xinjiang Medical University, Urumqi, 830011, China.
authors.forename: Renaguli
authors.initials: R
authors.lastname: Reyimu
authors.affiliation: Uyghur Medical College, Xinjiang Medical University, Urumqi, 830011, China. halmurat@263.net.
authors.forename: Halmurat
authors.initials: H
authors.lastname: Upur
authors.affiliation: Central Laboratory of Xinjiang Medical University, Urumqi, 830011, China. ainiwa@sina.com.
authors.forename: Ainiwaer
authors.initials: A
authors.lastname: Aikemu
mesh_terms: D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000627:therapeutic use*; D004354:Drug Screening Assays, Antitumor; D005260:Female; D006367:HeLa Cells; D006801:Humans; D008297:Male; D051379:Mice; D009368:Neoplasm Transplantation; D009929:Organ Size / Q000187:drug effects; D010936:Plant Extracts / Q000627:therapeutic use*; D053773:Transforming Growth Factor beta1 / Q000096:biosynthesis; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis; D002583:Uterine Cervical Neoplasms / Q000188:drug therapy* / Q000473:pathology
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D010936:Plant Extracts; D053773:Transforming Growth Factor beta1; D014409:Tumor Necrosis Factor-alpha; C000600923:abnormal Savda Munziq