title: Promicromonospora kermanensis sp. nov., an actinobacterium isolated from soil.
abstract: A novel strain belonging to the genus Promicromonospora, designated HM 533T, was isolated from soil in Kerman Province, Iran. It produced long and branched hyphae on ISP 2 medium that developed into a large number of irregular-shaped spores. It showed optimal growth at 25-30 °C and pH 5.0-8.0 with 0-4 % (w/v) NaCl. The peptidoglycan type of strain HM 533T was A4α l-Lys-l-Ala-d-Glu. Whole-cell hydrolysates of strain HM 533T contained the sugars ribose, glucose and galactose. The main phospholipids were phosphatidylglycerol, diphosphatidylglycerol, phosphatidylinositol, two unknown glycolipids and an unknown phospholipid. MK-9(H4) and MK-9(H2) were the predominant menaquinones. The fatty acids pattern was mainly composed of the saturated branched-chain acids anteiso-C15 : 0 and iso-C15 : 0. The 16S rRNA gene sequence analysis showed the highest pairwise sequence identity (99.5-97.1 %) with the members of the genus Promicromonospora. Based on phenotypic and genotypic features, strain HM 533T is considered to represent a novel species of the genus Promicromonospora, for which the name Promicromonospora kermanensis is proposed with strain HM 533T (=DSM 45485T=UTMC 00533T=CECT 8709T) as the type strain.
authors.affiliation: Microbial Biotechnology Department, School of Biology and Center of Excellence in Phylogeny of Living Organisms, College of Science, University of Tehran, Tehran 14155-6455, Iran.
authors.forename: Fatemeh
authors.initials: F
authors.lastname: Mohammadipanah
authors.affiliation: School of Biology, Newcastle University, Ridley Building, Newcastle upon Tyne, NE1 7RU, UK.|Leibniz-Institute DSMZ - German Collection of Microorganisms and Cell Cultures, Inhoffenstrasse 7b, Braunschweig 38124, Germany.
authors.forename: Maria Del Carmen
authors.initials: MDC
authors.lastname: Montero-Calasanz
authors.affiliation: Leibniz-Institute DSMZ - German Collection of Microorganisms and Cell Cultures, Inhoffenstrasse 7b, Braunschweig 38124, Germany.
authors.forename: Peter
authors.initials: P
authors.lastname: Schumann
authors.affiliation: Leibniz-Institute DSMZ - German Collection of Microorganisms and Cell Cultures, Inhoffenstrasse 7b, Braunschweig 38124, Germany.
authors.forename: Cathrin
authors.initials: C
authors.lastname: Spröer
authors.affiliation: Helmholtz Centre for Infection Research, Central Facility for Microscopy, Inhoffenstrasse 7, Braunschweig 38124, Germany.
authors.forename: Manfred
authors.initials: M
authors.lastname: Rohde
authors.affiliation: School of Biology, Newcastle University, Ridley Building, Newcastle upon Tyne, NE1 7RU, UK.|Leibniz-Institute DSMZ - German Collection of Microorganisms and Cell Cultures, Inhoffenstrasse 7b, Braunschweig 38124, Germany.
authors.forename: Hans-Peter
authors.initials: HP
authors.lastname: Klenk
mesh_terms: D000192:Actinomycetales / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D015373:Bacterial Typing Techniques; D001482:Base Composition; D002473:Cell Wall / Q000737:chemistry; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D006017:Glycolipids / Q000737:chemistry; D007492:Iran; D010457:Peptidoglycan / Q000737:chemistry; D010743:Phospholipids / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D006017:Glycolipids; D010457:Peptidoglycan; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; C073355:menaquinone 9