title: Changes in nitric oxide/hydrogen peroxide content and cell cycle progression: Study with synchronized cultures of green alga Chlamydomonas reinhardtii.
abstract: The present study aimed to evaluate the possible relationship between the changes in hydrogen peroxide (H2O2) and nitric oxide (NO) content and the course of growth and reproductive processes of the cell cycle of Chlamydomonas reinhardtii. The peak of H2O2observed at the beginning of the cell cycle was found to originate from Fe-SOD and Mn-SODchl.activity and result from the alternation in the photosynthetic processes caused by the dark-to-light transition of daughter cells. A rapid increase in NO concentration, observed before the light-to-dark cell transition, originated from NR and NIR activity and was followed by a photosynthesis-independent, Mn-SODchl.-mediated increases in H2O2production. This H2O2peak overlapped the beginning of Chlamydomonas cell division, which was indicated by a profile of CYCs and CDKs characteristic of cells' passage through the G1/S and S/M checkpoints. Taken together, our results show that there is a clear relationship between the course of the Chlamydomonas cell cycle and typical changes in the H2O2/NO ratio, as well as changes in expression and activity of enzymes involved in generation and scavenging of these signaling molecules.
authors.affiliation: Department of Plant Physiology and Biotechnology, University of Gdańsk, ul. Wita Stwosza 59, 80-308 Gdańsk, Poland. Electronic address: pokora@ug.edu.pl.
authors.forename: Wojciech
authors.initials: W
authors.lastname: Pokora
authors.affiliation: Department of Plant Physiology and Biotechnology, University of Gdańsk, ul. Wita Stwosza 59, 80-308 Gdańsk, Poland.
authors.forename: Anna
authors.initials: A
authors.lastname: Aksmann
authors.affiliation: Department of Plant Physiology and Biotechnology, University of Gdańsk, ul. Wita Stwosza 59, 80-308 Gdańsk, Poland.
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Baścik-Remisiewicz
authors.affiliation: Department of Biochemistry, Medical University of Gdańsk, ul. Dębinki 1, 80-211 Gdańsk, Poland.
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Dettlaff-Pokora
authors.affiliation: Department of Plant Physiology and Biotechnology, University of Gdańsk, ul. Wita Stwosza 59, 80-308 Gdańsk, Poland.
authors.forename: Max
authors.initials: M
authors.lastname: Rykaczewski
authors.affiliation: Department of Plant Physiology and Biotechnology, University of Gdańsk, ul. Wita Stwosza 59, 80-308 Gdańsk, Poland.
authors.forename: Magdalena
authors.initials: M
authors.lastname: Gappa
authors.affiliation: Department of Plant Physiology and Biotechnology, University of Gdańsk, ul. Wita Stwosza 59, 80-308 Gdańsk, Poland.
authors.forename: Zbigniew
authors.initials: Z
authors.lastname: Tukaj
mesh_terms: D002453:Cell Cycle / Q000502:physiology*; D016825:Chlamydomonas reinhardtii / Q000254:growth & development / Q000502:physiology* / Q000528:radiation effects; D006861:Hydrogen Peroxide / Q000378:metabolism*; D008027:Light; D009569:Nitric Oxide / Q000378:metabolism*; D010788:Photosynthesis; D010940:Plant Proteins / Q000378:metabolism; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D010940:Plant Proteins; D009569:Nitric Oxide; D006861:Hydrogen Peroxide; D013482:Superoxide Dismutase
keywords: Cell cycle; Chlamydomonas; Hydrogen peroxide; Nitric oxide