title: MiR-101 targets USP22 to inhibit the tumorigenesis of papillary thyroid carcinoma.
abstract: Increasing evidence suggests that microRNA-101 (miR-101) is involved in the progression of various human cancers, including papillary thyroid carcinoma (PTC). However, the biological functions of miR-101 and underlying molecular mechanisms in PTC remain largely unknown. In this study, we demonstrated that miR-101 underexpression in PTC tissue was associated with lymph node metastasis and poor prognosis of PTC patients. MiR-101 reduced PTC cell proliferation, apoptosis resistance, and invasion. Ubiquitin-specific protease 22 (USP22) was confirmed as a direct target of miR-101. USP22 restoration attenuated the inhibitory effects of miR-101 on PTC malignant traits in vitro. In vivo, miR-101 overexpression or USP22 depletion reduced the tumorigenesis of PTC. Overall, our findings provide new insight into the mechanism of PTC inhibition by miR-101, suggesting the potential of miR-101 as a therapeutic target in PTC patients.
authors.affiliation: Department of General Surgery, Tangdu Hospital, The Fourth Military Medical University Xi'an 710032, Shaanxi, China.
authors.forename: Huadong
authors.initials: H
authors.lastname: Zhao
authors.affiliation: Department of General Surgery, Tangdu Hospital, The Fourth Military Medical University Xi'an 710032, Shaanxi, China.
authors.forename: Haili
authors.initials: H
authors.lastname: Tang
authors.affiliation: Department of Hepatobiliary Surgery, Xijing Hospital, The Fourth Military Medical University Xi'an 710032, Shaanxi, China.
authors.forename: Qike
authors.initials: Q
authors.lastname: Huang
authors.affiliation: Department of General Surgery, Tangdu Hospital, The Fourth Military Medical University Xi'an 710032, Shaanxi, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Qiu
authors.affiliation: Department of General Surgery, Tangdu Hospital, The Fourth Military Medical University Xi'an 710032, Shaanxi, China.
authors.forename: Xiaomin
authors.initials: X
authors.lastname: Liu
authors.affiliation: Department of General Surgery, Tangdu Hospital, The Fourth Military Medical University Xi'an 710032, Shaanxi, China.
authors.forename: Dong
authors.initials: D
authors.lastname: Fan
authors.affiliation: Department of Pathology, Tangdu Hospital, The Fourth Military Medical University Xi'an 710032, Shaanxi, China.
authors.forename: Li
authors.initials: L
authors.lastname: Gong
authors.affiliation: Department of Anesthesiology, PLA Army General Hospital Beijing 100700, China.
authors.forename: Hang
authors.initials: H
authors.lastname: Guo
authors.affiliation: Department of Neurosurgery, 451th Central Hospital of PLA Xi'an 710054, Shaanxi, China.
authors.forename: Chong
authors.initials: C
authors.lastname: Chen
authors.affiliation: Department of General Surgery, Tangdu Hospital, The Fourth Military Medical University Xi'an 710032, Shaanxi, China.
authors.forename: Shixiong
authors.initials: S
authors.lastname: Lei
authors.affiliation: Department of Dermatology, Tangdu Hospital, The Fourth Military Medical University Xi'an 710032, Shaanxi, China.
authors.forename: Lu
authors.initials: L
authors.lastname: Yang
authors.affiliation: Department of General Surgery, Tangdu Hospital, The Fourth Military Medical University Xi'an 710032, Shaanxi, China.
authors.forename: Jianguo
authors.initials: J
authors.lastname: Lu
authors.affiliation: Department of General Surgery, Tangdu Hospital, The Fourth Military Medical University Xi'an 710032, Shaanxi, China.
authors.forename: Guoqiang
authors.initials: G
authors.lastname: Bao
keywords: MicroRNA-101; papillary thyroid carcinoma; tumorigenesis; ubiquitin-specific protease 22