title: A De-O-acylated Lipooligosaccharide-Based Adjuvant System Promotes Antibody and Th1-Type Immune Responses to H1N1 Pandemic Influenza Vaccine in Mice.
abstract: Vaccine adjuvants are agents that are used to promote immune responses to vaccine antigens and thereby to enhance the protective efficacy of the vaccines. In this study, we investigated the adjuvant activity of CIA06, an adjuvant system that is composed of a toll-like receptor 4 agonist de-O-acylated lipooligosaccharide (dLOS) and aluminum hydroxide, on the H1N1 pandemic influenza vaccine Greenflu-S® in mice. CIA06 significantly enhanced influenza-specific serum IgG, hemagglutination-inhibition, and virus-neutralizing antibody titers, which eliminated vaccine dose-dependency in the antibody response. Mice immunized with the CIA06-adjuvanted Greenflu-S showed Th1-type-predominant cytokine profiles, and both CD4+and CD8+T cell responses were induced. Immunization of mice with the CIA06-adjuvanted vaccine reduced the mortality and morbidity of mice upon lethal challenges with influenza virus, and no excessive inflammatory responses were observed in the lung tissues of the immunized mice after viral infection. These data suggest that the dLOS-based adjuvant system CIA06 can be used to promote the immune responses to influenza vaccine or to spare antigen dose without causing harmful inflammatory responses.
authors.affiliation: Department of Bioscience and Biotechnology, Sejong University, Seoul, Republic of Korea.
authors.forename: Ji In
authors.initials: JI
authors.lastname: Ryu
authors.affiliation: Department of Bioscience and Biotechnology, Sejong University, Seoul, Republic of Korea.
authors.forename: Shin Ae
authors.initials: SA
authors.lastname: Park
authors.affiliation: Department of Bioscience and Biotechnology, Sejong University, Seoul, Republic of Korea.
authors.forename: Seo Ri
authors.initials: SR
authors.lastname: Wui
authors.affiliation: Department of Bioscience and Biotechnology, Sejong University, Seoul, Republic of Korea.
authors.forename: Ara
authors.initials: A
authors.lastname: Ko
authors.affiliation: Department of Bioscience and Biotechnology, Sejong University, Seoul, Republic of Korea.
authors.forename: Ji Eun
authors.initials: JE
authors.lastname: Han
authors.affiliation: Laboratory Science Division, International Vaccine Institute, Seoul, Republic of Korea.
authors.forename: Jung Ah
authors.initials: JA
authors.lastname: Choi
authors.affiliation: Laboratory Science Division, International Vaccine Institute, Seoul, Republic of Korea.
authors.forename: Man Ki
authors.identifier: 0000-0002-8279-9041
authors.initials: MK
authors.lastname: Song
authors.affiliation: R&D Center, EyeGene, Seoul, Republic of Korea.
authors.forename: Kwang Sung
authors.initials: KS
authors.lastname: Kim
authors.affiliation: R&D Center, EyeGene, Seoul, Republic of Korea.
authors.forename: Yang Je
authors.initials: YJ
authors.lastname: Cho
authors.affiliation: Department of Bioscience and Biotechnology, Sejong University, Seoul, Republic of Korea.
authors.forename: Na Gyong
authors.identifier: 0000-0001-6842-9995
authors.initials: NG
authors.lastname: Lee
mesh_terms: D000276:Adjuvants, Immunologic / Q000008:administration & dosage*; D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D005260:Female; D053118:Influenza A Virus, H1N1 Subtype / Q000276:immunology*; D007252:Influenza Vaccines / Q000276:immunology*; D008070:Lipopolysaccharides / Q000276:immunology*; D008168:Lung / Q000473:pathology; D051379:Mice; D008807:Mice, Inbred BALB C; D009976:Orthomyxoviridae Infections / Q000276:immunology* / Q000517:prevention & control; D011014:Pneumonia / Q000473:pathology; D018417:Th1 Cells / Q000276:immunology
chemical_list: D000276:Adjuvants, Immunologic; D000914:Antibodies, Viral; D007252:Influenza Vaccines; D008070:Lipopolysaccharides; C023023:lipid-linked oligosaccharides