title: Lignans from Opuntia ficus-indica seeds protect rat primary hepatocytes and HepG2 cells against ethanol-induced oxidative stress.
abstract: Bioactivity-guided isolation of Opuntia ficus-indica (Cactaceae) seeds against ethanol-treated primary rat hepatocytes yielded six lignan compounds. Among the isolates, furofuran lignans 4-6, significantly protected rat hepatocytes against ethanol-induced oxidative stress by reducing intracellular reactive oxygen species levels, preserving antioxidative defense enzyme activities, and maintaining the glutathione content. Moreover, 4 dose-dependently induced the heme oxygenase-1 expression in HepG2 cells.
authors.affiliation: a College of Pharmacy and Research Institute of Pharmaceutical Science, Seoul National University , Gwanak-Ku , Republic of Korea.
authors.forename: Jung Wha
authors.initials: JW
authors.lastname: Kim
authors.affiliation: b College of Pharmacy, Kangwon National University , Chuncheon , Republic of Korea.
authors.forename: Heejung
authors.initials: H
authors.lastname: Yang
authors.affiliation: a College of Pharmacy and Research Institute of Pharmaceutical Science, Seoul National University , Gwanak-Ku , Republic of Korea.
authors.forename: Hyeon Woo
authors.initials: HW
authors.lastname: Kim
authors.affiliation: c College of Pharmacy, Ajou University , Suwon , Republic of Korea.
authors.forename: Hong Pyo
authors.initials: HP
authors.lastname: Kim
authors.affiliation: a College of Pharmacy and Research Institute of Pharmaceutical Science, Seoul National University , Gwanak-Ku , Republic of Korea.
authors.forename: Sang Hyun
authors.initials: SH
authors.lastname: Sung
mesh_terms: D000818:Animals; D019610:Cytoprotection / Q000187:drug effects*; D000431:Ethanol / Q000009:adverse effects*; D056945:Hep G2 Cells; D022781:Hepatocytes / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D006801:Humans; D017705:Lignans / Q000302:isolation & purification / Q000494:pharmacology*; D029747:Opuntia / Q000737:chemistry*; D018384:Oxidative Stress / Q000187:drug effects*; D051381:Rats; D012639:Seeds / Q000737:chemistry*
chemical_list: D017705:Lignans; D000431:Ethanol
keywords: Opuntia ficus-indica; heme oxygenase-1; hepatoprotective activity; lignin