title: Development of catecholamine and cortisol stress responses in zebrafish.
abstract: Both adrenal catecholamines and steroids are known to be involved in the stress response, immune function, blood pressure and energy homeostasis. The response to stress is characterized by the activation of the hypothalamus-pituitary-adrenal (HPA) axis and the sympathetic-adrenomedullary system, though the correlation with activation and development is not well understood. We evaluated the stress response of both cortisol and catecholamines during development in zebrafish. Zebrafish at two different stages of development were stressed in one of two different ways and cortisol and catecholamine were measured. Cortisol was measured by enzyme immune assay and catecholamine was measured by ELISA. Our results show that stress responses are delayed until after the synthesis of both cortisol and catecholamines. These observations suggest that the development of HPA axis may be required for the acquisition of the stress response for cortisol and catecholamines.
authors.affiliation: Department of Pediatrics, University of California, Los Angeles, USA; Department of Pediatrics, Tokyo Women's Medical University, Tokyo, Japan.
authors.forename: Kaoru
authors.initials: K
authors.lastname: Eto
authors.affiliation: Department of Bioengineering, University of California, Los Angeles, USA.
authors.forename: Jaime K
authors.initials: JK
authors.lastname: Mazilu-Brown
authors.affiliation: Department of Pediatrics, University of California, Los Angeles, USA.
authors.forename: Nicole
authors.initials: N
authors.lastname: Henderson-MacLennan
authors.affiliation: Department of Pediatrics, University of California, Los Angeles, USA; Department of Human Genetics, University of California, Los Angeles, USA.
authors.forename: Katrina M
authors.initials: KM
authors.lastname: Dipple
authors.affiliation: Department of Pediatrics, University of California, Los Angeles, USA; Department of Bioengineering, University of California, Los Angeles, USA; Department of Human Genetics, University of California, Los Angeles, USA; Department of Pediatrics, University of Colorado School of Medicine, Aurora, CO, USA.
authors.forename: Edward R B
authors.initials: ER
authors.lastname: McCabe
keywords: Catecholamines; Cortisol; HPA axis; Stress response; Zebrafish