title: A simple method to ensure homogeneous drug distribution during intrarenal infusion.
abstract: Intrarenal drug infusion plays an important role in renal experimental research. Laminar flow of the blood can cause streaming and inhomogeneous intrarenal distribution of infused drugs. We suggest a simple method to achieve a homogeneous intravascular distribution of drugs infused into the renal artery of anesthetized rats. The method employs a multiple sidehole catheter inserted into the renal artery, which enables an efficient drug mixing with the arterial blood. To verify the efficiency of this method, we use laser speckle imaging and renal artery flowmetry. The results show that, compared with the conventional single-hole catheter, the multiple sidehole catheter provides a more uniform drug distribution and a homogenous vascular response on the surface of the kidney.
authors.affiliation: Biomedical Sciences Institute, Copenhagen University, Copenhagen, Denmark dpostnov@sund.ku.dk.
authors.forename: Dmitry D
authors.initials: DD
authors.lastname: Postnov
authors.affiliation: Biomedical Sciences Institute, Copenhagen University, Copenhagen, Denmark.
authors.forename: Max
authors.initials: M
authors.lastname: Salomonsson
authors.affiliation: Biomedical Sciences Institute, Copenhagen University, Copenhagen, Denmark.
authors.forename: Charlotte M
authors.initials: CM
authors.lastname: Sorensen
authors.affiliation: Biomedical Sciences Institute, Copenhagen University, Copenhagen, Denmark.
authors.forename: Olga
authors.initials: O
authors.lastname: Sosnovtseva
mesh_terms: D000804:Angiotensin II / Q000008:administration & dosage* / Q000097:blood; D000818:Animals; D001783:Blood Flow Velocity; D002406:Catheterization, Peripheral / Q000295:instrumentation / Q000379:methods*; D004867:Equipment Design; D007261:Infusions, Intra-Arterial; D007668:Kidney / Q000098:blood supply*; D017078:Laser-Doppler Flowmetry; D008297:Male; D008955:Models, Cardiovascular; D055420:Perfusion Imaging / Q000379:methods; D017207:Rats, Sprague-Dawley; D012077:Renal Artery / Q000187:drug effects* / Q000502:physiology; D012079:Renal Circulation / Q000187:drug effects*; D013997:Time Factors; D062666:Vascular Access Devices; D014661:Vasoconstriction / Q000187:drug effects*; D014662:Vasoconstrictor Agents / Q000008:administration & dosage* / Q000097:blood
chemical_list: D014662:Vasoconstrictor Agents; D000804:Angiotensin II
keywords: blood flow imagery; drug delivery; kidney; laser speckle; renal hemodynamics