title: Diversity and resilience of the wood-feeding higher termiteMironasutitermes shangchengensisgut microbiota in response to temporal and diet variations.
abstract: Termites are considered among the most efficient bioreactors, with high capacities for lignocellulose degradation and utilization. Recently, several studies have characterized the gut microbiota of diverse termites. However, the temporal dynamics of the gut microbiota within a given termite with dietary diversity are poorly understood. Here, we employed 16S rDNA barcoded pyrosequencing analysis to investigate temporal changes in bacterial diversity and richness of the gut microbiota of wood-feeding higher termiteMironasutitermes shangchengensisunder three lignocellulose content-based diets that feature wood, corn stalks, and filter paper. Compositions of the predominant termite gut residents were largely constant among the gut microbiomes under different diets, but each diet caused specific changes in the bacterial composition over time. Notably, microbial communities exhibited an unexpectedly strong resilience during continuous feeding on both corn stalks and filter paper. Members of five bacterial phyla, that is, Spirochaetes, Firmicutes, Actinobacteria, Tenericutes, and Acidobacteria, were strongly associated with the resilience. These findings provide insights into the stability of the gut microbiota in higher termites and have important implications for the future design of robust bioreactors for lignocellulose degradation and utilization.
authors.affiliation: College of Life Sciences Henan Agricultural University Zhengzhou Henan China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Wang
authors.affiliation: College of Life Sciences Henan Agricultural University Zhengzhou Henan China.
authors.forename: Lijuan
authors.initials: L
authors.lastname: Su
authors.affiliation: Single-Cell Center CAS Key Laboratory of Biofuels and Shandong Key Laboratory of Energy Genetics Qingdao Institute of Bioenergy and Bioprocess Technology Chinese Academy of Sciences Qingdao Shandong China.
authors.forename: Shi
authors.initials: S
authors.lastname: Huang
authors.affiliation: Single-Cell Center CAS Key Laboratory of Biofuels and Shandong Key Laboratory of Energy Genetics Qingdao Institute of Bioenergy and Bioprocess Technology Chinese Academy of Sciences Qingdao Shandong China.
authors.forename: Cunpei
authors.initials: C
authors.lastname: Bo
authors.affiliation: College of Life Sciences Henan Agricultural University Zhengzhou Henan China.
authors.forename: Sen
authors.initials: S
authors.lastname: Yang
authors.affiliation: College of Life Sciences Henan Agricultural University Zhengzhou Henan China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Li
authors.affiliation: College of Life Sciences Henan Agricultural University Zhengzhou Henan China; Key Laboratory of Enzyme Engineering of Agricultural Microbiology Ministry of Agriculture Zhengzhou Henan China.
authors.forename: Fengqin
authors.initials: F
authors.lastname: Wang
authors.affiliation: College of Life Sciences Henan Agricultural University Zhengzhou Henan China; Key Laboratory of Enzyme Engineering of Agricultural Microbiology Ministry of Agriculture Zhengzhou Henan China.
authors.forename: Hui
authors.initials: H
authors.lastname: Xie
authors.affiliation: Single-Cell Center CAS Key Laboratory of Biofuels and Shandong Key Laboratory of Energy Genetics Qingdao Institute of Bioenergy and Bioprocess Technology Chinese Academy of Sciences Qingdao Shandong China.
authors.forename: Jian
authors.initials: J
authors.lastname: Xu
authors.affiliation: College of Life Sciences Henan Agricultural University Zhengzhou Henan China; Key Laboratory of Enzyme Engineering of Agricultural Microbiology Ministry of Agriculture Zhengzhou Henan China.
authors.forename: Andong
authors.initials: A
authors.lastname: Song
keywords: Mironasutitermes shangchengensis; barcoded pyrosequencing; core microbiome; resilience; temporal disturbance