title: Platinum(ii) O,S complexes as potential metallodrugs against Cisplatin resistance.
abstract: We report on platinum(ii) complexes with different cinnamic acid derivatives as ligands with cytotoxic activity against Cisplatin resistant ovarian cancer cell line subcultures of SKOV3 and A2780. A typical mechanism of action for platinum(ii) complexes as Cisplatin itself is binding to the DNA and inducing double-strand breaks. We examined the biological behavior of these potential drugs with 9-methylguanine using NMR spectroscopic methods and their DNA damage potential including γH2AX-foci analyses. X-ray diffraction methods have been used to elucidate the molecular structures of the platinum(ii) complexes. Interactions with the model protein lysozyme have been evaluated by different techniques including UV-Vis absorption spectroscopy, fluorescence and X-ray crystallography.
authors.affiliation: Institut für Anorganische und Analytische Chemie Friedrich-Schiller-Universität Jena Humboldtstraße 8, 07743 Jena, Germany. wolfgang.weigand@uni-jena.de.
authors.forename: Jana
authors.initials: J
authors.lastname: Hildebrandt
authors.affiliation: Department of Gynecology, Jena University Hospital - Friedrich Schiller University Jena, Germany.
authors.forename: Norman
authors.initials: N
authors.lastname: Häfner
authors.affiliation: Institut für Anorganische und Analytische Chemie Friedrich-Schiller-Universität Jena Humboldtstraße 8, 07743 Jena, Germany. wolfgang.weigand@uni-jena.de.
authors.forename: Helmar
authors.initials: H
authors.lastname: Görls
authors.affiliation: Department of Gynecology, Jena University Hospital - Friedrich Schiller University Jena, Germany.
authors.forename: Daniel
authors.initials: D
authors.lastname: Kritsch
authors.affiliation: Department of Chemical Sciences, University of Naples Federico II, Complesso Universitario di Monte Sant' Angelo, Via Cintia, I-80126, Napoli, Italy.
authors.forename: Giarita
authors.initials: G
authors.lastname: Ferraro
authors.affiliation: Department of Gynecology, Jena University Hospital - Friedrich Schiller University Jena, Germany.
authors.forename: Matthias
authors.initials: M
authors.lastname: Dürst
authors.affiliation: Department of Gynecology, Jena University Hospital - Friedrich Schiller University Jena, Germany.
authors.forename: Ingo B
authors.initials: IB
authors.lastname: Runnebaum
authors.affiliation: Department of Chemical Sciences, University of Naples Federico II, Complesso Universitario di Monte Sant' Angelo, Via Cintia, I-80126, Napoli, Italy and CNR Institute of Biostructures and Bioimages, Via Mezzocannone 16, I-80100, Napoli, Italy. antonello.merlino@unina.it.
authors.forename: Antonello
authors.initials: A
authors.lastname: Merlino
authors.affiliation: Institut für Anorganische und Analytische Chemie Friedrich-Schiller-Universität Jena Humboldtstraße 8, 07743 Jena, Germany. wolfgang.weigand@uni-jena.de.
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Weigand
mesh_terms: D000970:Antineoplastic Agents / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D002934:Cinnamates / Q000138:chemical synthesis / Q000737:chemistry; D002945:Cisplatin / Q000494:pharmacology*; D004249:DNA Damage; D019008:Drug Resistance, Neoplasm / Q000187:drug effects*; D006801:Humans; D008024:Ligands; D015394:Molecular Structure; D009944:Organoplatinum Compounds / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*
chemical_list: D000970:Antineoplastic Agents; D002934:Cinnamates; D008024:Ligands; D009944:Organoplatinum Compounds; C029010:cinnamic acid; D002945:Cisplatin