title: Inhibitory effect of phloretin on α-glucosidase: Kinetics, interaction mechanism and molecular docking.
abstract: As the aglycone of phloridzin, phloretin belongs to dihydrochalcone with antioxidant, anti-inflammatory and antimicrobial activities. In this study, multispectroscopic techniques and molecular docking analysis were used to investigate the inhibitory activity and mechanisms of phloretin on α-glucosidase. The results showed that phloretin reversibly inhibited α-glucosidase in a mixed-type manner and the value of IC50was 31.26μgL-1. The intrinsic fluorescence of α-glucosidase was quenched by the interactions with phloretin through a static quenching mechanism and spontaneously formed phloretin-α-glucosidase complex by the driving forces of van der Waals force and hydrogen bond. Atomic force microscope (AFM) studies and FT-IR measurements suggested that the interactions could change the micro-environments and conformation of the enzymes and the molecular docking analysis displayed the exact binding site of phloretin on α-glucosidase. These results indicated that phloretin is a strong α-glucosidase inhibitor, thus could be contribute to the improvement of diabetes mellitus.
authors.affiliation: College of Food Science and Engineering, Northwest A&F University, Yangling 712100, PR China; College of Life Science and Engineering, Chongqing Three Gorges University, Chongqing 404100, PR China.
authors.forename: Lin
authors.initials: L
authors.lastname: Han
authors.affiliation: College of Food Science and Engineering, Northwest A&F University, Yangling 712100, PR China.
authors.forename: Chun
authors.initials: C
authors.lastname: Fang
authors.affiliation: College of Food Science and Engineering, Northwest A&F University, Yangling 712100, PR China.
authors.forename: Ruixue
authors.initials: R
authors.lastname: Zhu
authors.affiliation: College of Food Science and Engineering, Northwest A&F University, Yangling 712100, PR China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Peng
authors.affiliation: College of Science, Northwest A&F University, Yangling 712100, PR China.
authors.forename: Ding
authors.initials: D
authors.lastname: Li
authors.affiliation: College of Food Science and Engineering, Northwest A&F University, Yangling 712100, PR China. Electronic address: wangmin20050606@163.com.
authors.forename: Min
authors.initials: M
authors.lastname: Wang
mesh_terms: D065089:Glycoside Hydrolase Inhibitors / Q000378:metabolism* / Q000494:pharmacology*; D007700:Kinetics; D062105:Molecular Docking Simulation; D010693:Phloretin / Q000378:metabolism* / Q000494:pharmacology*; D011485:Protein Binding; D011487:Protein Conformation; D013816:Thermodynamics; D000520:alpha-Glucosidases / Q000737:chemistry / Q000378:metabolism*
chemical_list: D065089:Glycoside Hydrolase Inhibitors; D000520:alpha-Glucosidases; D010693:Phloretin
keywords: &alpha; -glucosidase; Inhibitory mechanisms; Phloretin