title: Single-molecule fluorescence resonance energy transfer in molecular biology.
abstract: Single-molecule fluorescence resonance energy transfer (smFRET) is a powerful technique for studying the conformation dynamics and interactions of individual biomolecules. In this review, we describe the concept and principle of smFRET, illustrate general instrumentation and microscopy settings for experiments, and discuss the methods and algorithms for data analysis. Subsequently, we review applications of smFRET in protein conformational changes, ion channel open-close properties, receptor-ligand interactions, nucleic acid structure regulation, vesicle fusion, and force induced conformational dynamics. Finally, we discuss the main limitations of smFRET in molecular biology.
authors.affiliation: The Institute for Molecular Engineering, The University of Chicago, Chicago, IL 60637, USA. huangjun@uchicago.edu sasmal@uchicago.edu.
authors.forename: Dibyendu K
authors.initials: DK
authors.lastname: Sasmal
authors.affiliation: The Institute for Molecular Engineering, The University of Chicago, Chicago, IL 60637, USA. huangjun@uchicago.edu sasmal@uchicago.edu.
authors.forename: Laura E
authors.initials: LE
authors.lastname: Pulido
authors.affiliation: The Institute for Molecular Engineering, The University of Chicago, Chicago, IL 60637, USA. huangjun@uchicago.edu sasmal@uchicago.edu.
authors.forename: Shan
authors.initials: S
authors.lastname: Kasal
authors.affiliation: The Institute for Molecular Engineering, The University of Chicago, Chicago, IL 60637, USA. huangjun@uchicago.edu sasmal@uchicago.edu.
authors.forename: Jun
authors.initials: J
authors.lastname: Huang
mesh_terms: D000465:Algorithms; D031541:Fluorescence Resonance Energy Transfer; D007473:Ion Channels / Q000737:chemistry; D008024:Ligands; D008968:Molecular Conformation; D036103:Nanotechnology; D009696:Nucleic Acids / Q000737:chemistry; D011506:Proteins / Q000737:chemistry
chemical_list: D007473:Ion Channels; D008024:Ligands; D009696:Nucleic Acids; D011506:Proteins