title: Characterization of isolates of equine infectious anemia virus in Brazil.
abstract: Equine infectious anemia is an important infectious disease that affects equids worldwide. Control of the disease is currently based on detection of anti-p26 EIAV by Agar Gel Immunodiffusion (AGID). In this work, 62 animals were examined by AGID and nested-PCR using primers for the gag gene. Fifty-three samples (85.5%) were positive by nested-PCR, whereas only 33 samples (53%) were positive for AGID. Fifteen amplicons obtained by nested-PCR were sequenced and the aligned results subjected to phylogenetic analysis. The analysis suggests that the Brazilian EIAV form a cluster with WSU5, EIAVUK and Wyoming strains from United States.
authors.affiliation: Laboratory of Virology, Institute of Health Science, Universidade Federal da Bahia (UFBA), Salvador, Brazil. dellanetigre@yahoo.com.br.|Department of Biologic Science, Universidade Estadual do Sudoeste da Bahia (UESB), Jequie, Brazil. dellanetigre@yahoo.com.br.
authors.forename: Dellane Martins
authors.initials: DM
authors.lastname: Tigre
authors.affiliation: Laboratory of Virology, Institute of Health Science, Universidade Federal da Bahia (UFBA), Salvador, Brazil.|Instituto Federal Baiano (IFBA), Santa Inês, Brazil.
authors.forename: Camila Fonseca Lopes
authors.initials: CF
authors.lastname: Brandão
authors.affiliation: Laboratory of Virology, Institute of Health Science, Universidade Federal da Bahia (UFBA), Salvador, Brazil.|Institute of Health Science, Universidade Federal do Recôncavo da Bahia (UFRB), Santo Antonio de Jesus, Brazil.
authors.forename: Fabiana Lopes
authors.initials: FL
authors.lastname: de Paula
authors.affiliation: Department of Biointeraction, Institute of Health Science, Universidade Federal da Bahia (UFBA), Salvador, Brazil.
authors.forename: Fabio Alexandre
authors.initials: FA
authors.lastname: Chinalia
authors.affiliation: Laboratory of Virology, Institute of Health Science, Universidade Federal da Bahia (UFBA), Salvador, Brazil.
authors.forename: Gubio Soares
authors.initials: GS
authors.lastname: Campos
authors.affiliation: Laboratory of Virology, Institute of Health Science, Universidade Federal da Bahia (UFBA), Salvador, Brazil.|Department of Biointeraction, Institute of Health Science, Universidade Federal da Bahia (UFBA), Salvador, Brazil.
authors.forename: Silvia Ines
authors.initials: SI
authors.lastname: Sardi
mesh_terms: D000818:Animals; D001938:Brazil; D004859:Equine Infectious Anemia / Q000821:virology*; D006736:Horses; D004860:Infectious Anemia Virus, Equine / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D014758:Viral Core Proteins / Q000235:genetics
chemical_list: D014758:Viral Core Proteins; C117895:equine infectious anemia virus, p26