title: Structures and Ribosomal Interaction of Ribosome-Inactivating Proteins.
abstract: Ribosome-inactivating proteins (RIPs) including ricin, Shiga toxin, and trichosanthin, are RNAN-glycosidases that depurinate a specific adenine residue (A-4324 in rat 28S ribosomal RNA, rRNA) in the conserved α-sarcin/ricin loop (α-SRL) of rRNA. RIPs are grouped into three types according to the number of subunits and the organization of the precursor sequences. RIPs are two-domain proteins, with the active site located in the cleft between the N- and C-terminal domains. It has been found that the basic surface residues of the RIPs promote rapid and specific targeting to the ribosome and a number of RIPs have been shown to interact with the C-terminal regions of the P proteins of the ribosome. At present, the structural basis for the interaction of trichosanthin and ricin-A chain toward P2 peptide is known. This review surveys the structural features of the representative RIPs and discusses how they approach and interact with the ribosome.
authors.affiliation: Centre for Protein Science and Crystallography, School of Life Sciences, The Chinese University of Hong Kong, Shatin, N.T., Hong Kong, China. Shiww@cuhk.edu.hk.
authors.forename: Wei-Wei
authors.initials: WW
authors.lastname: Shi
authors.affiliation: Centre for Protein Science and Crystallography, School of Life Sciences, The Chinese University of Hong Kong, Shatin, N.T., Hong Kong, China. amandamak@gmail.com.
authors.forename: Amanda Nga-Sze
authors.initials: AN
authors.lastname: Mak
authors.affiliation: Centre for Protein Science and Crystallography, School of Life Sciences, The Chinese University of Hong Kong, Shatin, N.T., Hong Kong, China. kbwong@cuhk.edu.hk.
authors.forename: Kam-Bo
authors.initials: KB
authors.lastname: Wong
authors.affiliation: Centre for Protein Science and Crystallography, School of Life Sciences, The Chinese University of Hong Kong, Shatin, N.T., Hong Kong, China. pcshaw@cuhk.edu.hk.
authors.forename: Pang-Chui
authors.initials: PC
authors.lastname: Shaw
mesh_terms: D020134:Catalytic Domain; D008958:Models, Molecular; D015394:Molecular Structure; D011485:Protein Binding; D054730:Protein Interaction Domains and Motifs; D054788:Ribosome Inactivating Proteins / Q000737:chemistry* / Q000145:classification / Q000378:metabolism; D054657:Ribosome Subunits / Q000235:genetics / Q000378:metabolism; D012270:Ribosomes / Q000737:chemistry*; D013329:Structure-Activity Relationship; D013379:Substrate Specificity
chemical_list: D054788:Ribosome Inactivating Proteins
keywords: P proteins; RNA N-glycosidases; ribosomal interaction; ribosome; ribosome-inactivating proteins