title: Intracerebroventricular Administration of Amyloid β-protein Oligomers Selectively Increases Dorsal Hippocampal Dialysate Glutamate Levels in the Awake Rat.
abstract: Extensive evidence supports an important role for soluble oligomers of the amyloid β-protein (Aβ) in Alzheimer's Disease pathogenesis. In the present study we combined intracerebroventricular (icv) injections with brain microdialysis technology in the fully conscious rat to assess the effects of icv administered SDS-stable low-n Aβ oligomers (principally dimers and trimers) on excitatory and inhibitory amino acid transmission in the ipsilateral dorsal hippocampus. Microdialysis was employed to assess the effect of icv administration of Aβ monomers and Aβ oligomers on dialysate glutamate, aspartate and GABA levels in the dorsal hippocampus. Administration of Aβ oligomers was associated with a +183% increase (p.
authors.affiliation: Applied Neurotherapeutics Research Group, Conway Institute, University College Dublin, Dublin 4, Ireland.
authors.forename: Sean D
authors.initials: SD
authors.lastname: O'Shea
authors.affiliation: Laboratory for Neurodegenerative Research, Conway Institute, University College Dublin, Dublin 4, Ireland.
authors.forename: Imelda M
authors.initials: IM
authors.lastname: Smith
authors.affiliation: Applied Neurotherapeutics Research Group, Conway Institute, University College Dublin, Dublin 4, Ireland.
authors.forename: Olive M
authors.initials: OM
authors.lastname: McCabe
authors.affiliation: Applied Neurotherapeutics Research Group, Conway Institute, University College Dublin, Dublin 4, Ireland.
authors.forename: Michelle M
authors.initials: MM
authors.lastname: Cronin
authors.affiliation: Laboratory for Neurodegenerative Research, Conway Institute, University College Dublin, Dublin 4, Ireland. dominic.walsh@ucd.ie.
authors.forename: Dominic M
authors.initials: DM
authors.lastname: Walsh
authors.affiliation: Applied Neurotherapeutics Research Group, Conway Institute, University College Dublin, Dublin 4, Ireland. william.oconnor@ul.ie.|Graduate Medical School, University of Limerick, Limerick, Ireland. william.oconnor@ul.ie.|Materials and Surface Science Institute, University of Limerick, Limerick, Ireland. william.oconnor@ul.ie.
authors.forename: William T
authors.initials: WT
authors.lastname: O'Connor
keywords: Alzheimer’s Disease; Amyloid β-protein; GABA; Glutamate; Microdialysis; Release