title: Parecoxib inhibits glioblastoma cell proliferation, migration and invasion by upregulating miRNA-29c.
abstract: Glioblastoma (GBM) is one of the most lethal brain cancers worldwide, and there is an urgent need for development of novel therapeutic approaches. Parecoxib is a well-known cyclooxygenase-2 (COX-2) inhibitor, and had already been developed for postoperative analgesia with high efficacy and low adverse reaction. A recent study has suggested that parecoxib potently enhances immunotherapeutic efficacy of GBM, but its effects on GBM growth, migration and invasion have not previously been studied. In the present study, MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] and BrdU (5-bromo-2-deoxyuridine) incorporation assays were used to evaluate the cell proliferation of GBM cells. Wound-healing and transwell assays were preformed to analyze GBM cell migration and invasion, respectively. The results suggested that parecoxib inhibits cell proliferation, migration and invasion of GBM cells in a dose-dependent manner. RT-qPCR (real-time quantitative PCR) analysis demonstrated that miRNA-29c can be significantly induced by parecoxib. Furthermore, our data suggests that a miRNA-29c inhibitor can significantly attenuate parecoxib's effect on proliferation, migration and invasion of GBM. In conclusion, the present study suggests that parecoxib inhibits GBM cell proliferation, migration and invasion by upregulating miRNA-29c.
authors.affiliation: The State Key Laboratory of Medical Genetics, School of Life Sciences, Central South University, Changsha, Hunan, China, 410078.|Department of Neurosurgery, The Third Xiangya Hospital of Central South University, Changsha, Hunan, China, 410013.
authors.forename: Lin-Yong
authors.initials: LY
authors.lastname: Li
authors.affiliation: Department of Emergency, The Third Xiangya Hospital of Central South University, Changsha, Hunan, China, 410013.
authors.forename: Jie
authors.initials: J
authors.lastname: Xiao
authors.affiliation: Department of Neurosurgery, The Third Xiangya Hospital of Central South University, Changsha, Hunan, China, 410013.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Liu
authors.affiliation: The State Key Laboratory of Medical Genetics, School of Life Sciences, Central South University, Changsha, Hunan, China, 410078 xyyxyxiakun@163.com.
authors.forename: Kun
authors.identifier: http://orcid.org/0000-0002-1093-9236
authors.initials: K
authors.lastname: Xia
keywords: Cyclooxygenase-2; Glioblastoma; Invasion; Parecoxib; Proliferation; miRNA-29c