title: Re-engineering of Bacterial Luciferase; For New Aspects of Bioluminescence.
abstract: Bacterial luminescence is the end-product of biochemical reactions catalyzed by the luciferase enzyme. Nowadays, this fascinating phenomenon has been widely used as reporter and/or sensors to detect a variety of biological and environmental processes. The enhancement or diversification of the luciferase activities will increase the versatility of bacterial luminescence. Here, to establish the strategy for luciferase engineering, we summarized the identity and relevant roles of key amino acid residues modulating luciferase in Vibrio harveyi, a model luminous bacterium. The current opinions on crystal structures and the critical amino acid residues involved in the substrate binding sites and unstructured loop have been delineated. Based on these, the potential target residues and/or parameters for enzyme engineering were also suggested in limited scale. In conclusion, even though the accurate knowledge on the bacterial luciferase is yet to be reported, the structure-guided site-directed mutagenesis approaches targeting the regulatory amino acids will provide a useful platform to re-engineer the bacterial luciferase in the future.
authors.affiliation: aDivision of Biotechnology, Safetey, Environment and Life Science Institute, College of Environmental & Bioresources Sciences, Chonbuk National University, Iksan, 570-752, Korea.
authors.forename: Da-Som
authors.initials: DS
authors.lastname: Kim
authors.affiliation: Department of Horticulture, College of Agriculture & Life Sciences, Chonbuk National University, Jeonju, 561-756, Korea.
authors.forename: Jeong-Ran
authors.initials: JR
authors.lastname: Choi
authors.affiliation: Department of Horticulture, College of Agriculture & Life Sciences, Chonbuk National University, Jeonju, 561-756, Korea.
authors.forename: Jeong-Ae
authors.initials: JA
authors.lastname: Ko
authors.affiliation: aDivision of Biotechnology, Safetey, Environment and Life Science Institute, College of Environmental & Bioresources Sciences, Chonbuk National University, Iksan, 570-752, Korea.
authors.forename: Kangmin
authors.initials: K
authors.lastname: Kim
mesh_terms: D000447:Aldehydes / Q000378:metabolism; D000818:Animals; D055162:Biocatalysis; D005415:Flavins / Q000378:metabolism; D049453:Luciferases, Bacterial / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D049449:Luminescence; D015202:Protein Engineering / Q000379:methods*
chemical_list: D000447:Aldehydes; D005415:Flavins; D049453:Luciferases, Bacterial
keywords: Aldehyde; bacterial luciferase; bioluminescence.; flavin; mobile loop; protein engineering