title: SIRT2 regulates nuclear envelope reassembly through ANKLE2 deacetylation.
abstract: Sirtuin 2 (SIRT2) is an NAD-dependent deacetylase known to regulate microtubule dynamics and cell cycle progression. SIRT2 has also been implicated in the pathology of cancer, neurodegenerative diseases and progeria. Here, we show that SIRT2 depletion or overexpression causes nuclear envelope reassembly defects. We link this phenotype to the recently identified regulator of nuclear envelope reassembly ANKLE2. ANKLE2 acetylation at K302 and phosphorylation at S662 are dynamically regulated throughout the cell cycle by SIRT2 and are essential for normal nuclear envelope reassembly. The function of SIRT2 therefore extends beyond the regulation of microtubules to include the regulation of nuclear envelope dynamics.
authors.affiliation: Department of Biochemistry, Max F. Perutz Laboratories, University of Vienna, Dr Bohr-Gasse 9, Vienna 1030, Austria.|Department of Molecular Biotechnology, University of Applied Sciences FH Campus Wien, Helmut-Qualtinger-Gasse 2, 1030 Vienna, Austria.
authors.forename: Tanja
authors.initials: T
authors.lastname: Kaufmann
authors.affiliation: Department of Biochemistry, Max F. Perutz Laboratories, University of Vienna, Dr Bohr-Gasse 9, Vienna 1030, Austria.
authors.forename: Eva
authors.initials: E
authors.lastname: Kukolj
authors.affiliation: Department of Biochemistry, Max F. Perutz Laboratories, University of Vienna, Dr Bohr-Gasse 9, Vienna 1030, Austria.
authors.forename: Andreas
authors.initials: A
authors.lastname: Brachner
authors.affiliation: Department of Biochemistry, Max F. Perutz Laboratories, University of Vienna, Dr Bohr-Gasse 9, Vienna 1030, Austria.
authors.forename: Etienne
authors.initials: E
authors.lastname: Beltzung
authors.affiliation: Department of Biochemistry, Max F. Perutz Laboratories, University of Vienna, Dr Bohr-Gasse 9, Vienna 1030, Austria.
authors.forename: Melania
authors.initials: M
authors.lastname: Bruno
authors.affiliation: Department of Biochemistry, Max F. Perutz Laboratories, University of Vienna, Dr Bohr-Gasse 9, Vienna 1030, Austria.
authors.forename: Sebastian
authors.initials: S
authors.lastname: Kostrhon
authors.affiliation: Institute of Molecular Pathology, Dr Bohr-Gasse 7, Vienna 1030, Austria.
authors.forename: Susanne
authors.initials: S
authors.lastname: Opravil
authors.affiliation: Institute of Molecular Pathology, Dr Bohr-Gasse 7, Vienna 1030, Austria.
authors.forename: Otto
authors.initials: O
authors.lastname: Hudecz
authors.affiliation: Institute of Molecular Pathology, Dr Bohr-Gasse 7, Vienna 1030, Austria.
authors.forename: Karl
authors.initials: K
authors.lastname: Mechtler
authors.affiliation: Department of Biochemistry, Max F. Perutz Laboratories, University of Vienna, Dr Bohr-Gasse 9, Vienna 1030, Austria.
authors.forename: Graham
authors.initials: G
authors.lastname: Warren
authors.affiliation: Department of Biochemistry, Max F. Perutz Laboratories, University of Vienna, Dr Bohr-Gasse 9, Vienna 1030, Austria dea.slade@univie.ac.at.
authors.forename: Dea
authors.identifier: 0000-0002-0052-5910
authors.initials: D
authors.lastname: Slade
mesh_terms: D000107:Acetylation; D019921:Biotinylation; D002453:Cell Cycle; D053144:Cell Nucleus Shape; D002846:Chromatography, Affinity; D057809:HEK293 Cells; D006801:Humans; D008565:Membrane Proteins / Q000378:metabolism*; D008954:Models, Biological; D009685:Nuclear Envelope / Q000378:metabolism*; D009687:Nuclear Proteins / Q000378:metabolism*; D010766:Phosphorylation; D011485:Protein Binding; D040901:Proteomics; D056565:Sirtuin 2 / Q000378:metabolism*
chemical_list: C574972:ANKLE2 protein, human; D008565:Membrane Proteins; D009687:Nuclear Proteins; D056565:Sirtuin 2
keywords: ANKLE2; Cell cycle; Nuclear envelope; SIRT2