title: Regulation and Maintenance of an Adoptive T-Cell Dependent Memory B Cell Pool.
abstract: We investigated the ability of monoclonal B cells to restore primary and secondary T-cell dependent antibody responses in adoptive immune-deficient hosts. Priming induced B cell activation and expansion, AID expression, antibody production and the generation of IgM+IgG- and IgM-IgG+ antigen-experienced B-cell subsets that persisted in the lymphopenic environment by cell division. Upon secondary transfer and recall the IgM-IgG+ cells responded by the production of antigen-specific IgG while the IgM+ memory cells secreted mainly IgM and little IgG, but generated new B cells expressing germinal center markers. The recall responses were more efficient if the antigenic boost was delayed suggesting that a period of adaptation is necessary before the transferred cells are able to respond. Overall these findings indicate that reconstitution of a functional and complete memory pool requires transfer of all different antigen-experienced B cell subsets. We also found that the size of the memory B cell pool did not rely on the number of the responding naïve B cells, suggesting autonomous homeostatic controls for naïve and memory B cells. By reconstituting a stable memory B cell pool in immune-deficient hosts using a monoclonal high-affinity B cell population we demonstrate the potential value of B cell adoptive immunotherapy.
authors.affiliation: Institut Pasteur, Départment d'Immunologie, Unité de Biologie des Populations Lymphocytaires, Paris, France.|CNRS, URA1961, Paris, France.
authors.forename: Marie
authors.initials: M
authors.lastname: Anson
authors.affiliation: Institut Pasteur, Départment d'Immunologie, Unité de Biologie des Populations Lymphocytaires, Paris, France.|CNRS, URA1961, Paris, France.
authors.forename: Inês
authors.initials: I
authors.lastname: Amado
authors.affiliation: Institut Pasteur, Départment d'Immunologie, Unité de Biologie des Populations Lymphocytaires, Paris, France.|CNRS, URA1961, Paris, France.
authors.forename: Marie-Pierre
authors.initials: MP
authors.lastname: Mailhé
authors.affiliation: Institut Cochin, Inserm, U1016, Paris, France.|CNRS, UMR8104, Paris, France.|Université Paris Descartes, Sorbonne Paris Cité, Paris, France.
authors.forename: Emmanuel
authors.initials: E
authors.lastname: Donnadieu
authors.affiliation: Institut Pasteur, Départment d'Immunologie, Unité de Biologie des Populations Lymphocytaires, Paris, France.|CNRS, URA1961, Paris, France.
authors.forename: Sylvie
authors.initials: S
authors.lastname: Garcia
authors.affiliation: Institut Pasteur, Départment d'Immunologie, Unité de Biologie des Populations Lymphocytaires, Paris, France.|CNRS, URA1961, Paris, France.
authors.forename: François
authors.initials: F
authors.lastname: Huetz
authors.affiliation: Institut Pasteur, Départment d'Immunologie, Unité de Biologie des Populations Lymphocytaires, Paris, France.|CNRS, URA1961, Paris, France.
authors.forename: Antonio A
authors.initials: AA
authors.lastname: Freitas
mesh_terms: D000818:Animals; D000943:Antigens, Differentiation / Q000235:genetics / Q000276:immunology; D016175:B-Lymphocyte Subsets / Q000276:immunology*; D018858:Germinal Center / Q000276:immunology*; D007074:Immunoglobulin G / Q000235:genetics / Q000276:immunology*; D007075:Immunoglobulin M / Q000235:genetics / Q000276:immunology*; D007156:Immunologic Memory; D051379:Mice; D018345:Mice, Knockout; D013601:T-Lymphocytes / Q000276:immunology*
chemical_list: D000943:Antigens, Differentiation; D007074:Immunoglobulin G; D007075:Immunoglobulin M