title: Sodium Butyrate Reduces Colitogenic Immunoglobulin A-Coated Bacteria and Modifies the Composition of Microbiota in IL-10 Deficient Mice.
abstract: High levels of immunoglobulin A (IgA)-coated bacteria may have a role in driving inflammatory bowel disease (IBD). We therefore investigated the effect of sodium butyrate on microbiota in IBD prone interleukin (IL)-10-/-mice. At 8 weeks of age, mice were allocated into three groups (n= 4/group): normal (C57BL/6), IL-10-/-, and IL-10-/-treated with sodium butyrate (100 mM). Severity of colitis, inflammatory cytokine and short-chain fatty acid (SCFA) concentration in proximal colon contents, the percentage of IgA-coated bacteria and microbiota composition by 16S ribosomal RNA assessment of stool were measured after 4 weeks of treatment. Sodium butyrate ameliorated histological colitis and decreased levels of tumor necrosis factor (TNF)-α and IL-6 in IL-10-/-mice compared with those without treatment. At the phylum level, a reduction inBacteroidetesand an increase inFirmicutesin IL-10-/-mice treated with sodium butyrate were observed. Additionally,Prevotellaceaespecies were reduced in IL-10-/-mice treated with sodium butyrate as compared with those without treatment. The level of biodiversity was slightly increased and the amount of IgA-coated bacteria decreased in IL-10-/-mice treated with sodium butyrate compared with those without treatment. Our results indicate that sodium butyrate protects against colitis, possibly through modifying the gut microbiota, enriching biodiversity and reducing the amount of colitogenic IgA-coated bacteria in IL-10-/-mice.
authors.affiliation: Department of General Surgery, Jinling Hospital Affiliated to Southern Medical University, No. 305 East Zhongshan Rd., Nanjing 210002, China. tenghuiff@163.com.|Department of General Surgery, Jinling Hospital, Medical School of Nanjing University, Nanjing 210002, China. tenghuiff@163.com.
authors.forename: Tenghui
authors.initials: T
authors.lastname: Zhang
authors.affiliation: Department of General Surgery, Jinling Hospital, Medical School of Nanjing University, Nanjing 210002, China. dingchao19910521@126.com.
authors.forename: Chao
authors.initials: C
authors.lastname: Ding
authors.affiliation: Department of General Surgery, Jinling Hospital Affiliated to Southern Medical University, No. 305 East Zhongshan Rd., Nanjing 210002, China. zhao_ming_li@163.com.
authors.forename: Mingli
authors.initials: M
authors.lastname: Zhao
authors.affiliation: Department of General Surgery, Jinling Hospital, Medical School of Nanjing University, Nanjing 210002, China. d670677094@163.com.
authors.forename: Xujie
authors.initials: X
authors.lastname: Dai
authors.affiliation: Department of General Surgery, Jinling Hospital, Medical School of Nanjing University, Nanjing 210002, China. yang_jianbo@126.com.
authors.forename: Jianbo
authors.initials: J
authors.lastname: Yang
authors.affiliation: Department of General Surgery, Jinling Hospital, Medical School of Nanjing University, Nanjing 210002, China. liyi1860@126.com.
authors.forename: Yi
authors.initials: Y
authors.lastname: Li
authors.affiliation: Department of General Surgery, Jinling Hospital, Medical School of Nanjing University, Nanjing 210002, China. drgulili@163.com.
authors.forename: Lili
authors.initials: L
authors.lastname: Gu
authors.affiliation: Department of General Surgery, Jinling Hospital, Medical School of Nanjing University, Nanjing 210002, China. dr_betty@126.com.
authors.forename: Yao
authors.initials: Y
authors.lastname: Wei
authors.affiliation: Department of General Surgery, Jinling Hospital Affiliated to Southern Medical University, No. 305 East Zhongshan Rd., Nanjing 210002, China. gongjianfeng@aliyun.com.|Department of General Surgery, Jinling Hospital, Medical School of Nanjing University, Nanjing 210002, China. gongjianfeng@aliyun.com.
authors.forename: Jianfeng
authors.initials: J
authors.lastname: Gong
authors.affiliation: Department of General Surgery, Jinling Hospital, Medical School of Nanjing University, Nanjing 210002, China. dr_zhuweiming@126.com.
authors.forename: Weiming
authors.initials: W
authors.lastname: Zhu
authors.affiliation: Department of General Surgery, Jinling Hospital, Medical School of Nanjing University, Nanjing 210002, China. proflining@163.com.
authors.forename: Ning
authors.initials: N
authors.lastname: Li
authors.affiliation: Department of General Surgery, Jinling Hospital, Medical School of Nanjing University, Nanjing 210002, China. lijieshou@163.com.
authors.forename: Jieshou
authors.initials: J
authors.lastname: Li
mesh_terms: D000818:Animals; D020148:Butyric Acid / Q000494:pharmacology*; D003092:Colitis / Q000139:chemically induced / Q000382:microbiology; D003106:Colon; D005260:Female; D005766:Gastrointestinal Contents / Q000187:drug effects / Q000382:microbiology; D007070:Immunoglobulin A; D016753:Interleukin-10 / Q000235:genetics / Q000378:metabolism*; D007422:Intestines / Q000187:drug effects* / Q000382:microbiology; D008297:Male; D051379:Mice; D018345:Mice, Knockout; D064307:Microbiota / Q000187:drug effects*; D011897:Random Allocation; D013047:Specific Pathogen-Free Organisms
chemical_list: D007070:Immunoglobulin A; D020148:Butyric Acid; D016753:Interleukin-10
keywords: IL-10−/− mice; colitis; immunoglobulin A-coated bacteria; microbiota composition; sodium butyrate