title: Antibody-free detection of infectious bacteria using quantum dots-based barcode assay.
abstract: Staphylococcus aureus, methicillin-resistant Staphylococcus aureus and Klebsiella pneumoniae are the most representative bacteria causing infectious diseases. Due to the increased application of antibiotics, the bacterial resistance is growing causing severe complications. Therefore, a sensitive determination of these pathogens is crucial for effective treatment. The aim of this study was to design an effective method for multiplex detection of Staphylococcus aureus, methicillin-resistant Staphylococcus aureus and Klebsiella pneumoniae taking advantage from properties of magnetic particles as well as fluorescent nanoparticles (quantum dots). The method was able to detect as low concentrations of bacteria as 102CFU/mL using the bacteria-specific genes (fnbA, mecA and wcaG).
authors.affiliation: Department of Chemistry and Biochemistry, Mendel University in Brno, Zemedelska 1, CZ-613 00 Brno, Czechia; Central European Institute of Technology, Brno University of Technology, Purkynova 123, CZ-612 00 Brno, Czechia.
authors.forename: Kristyna
authors.initials: K
authors.lastname: Cihalova
authors.affiliation: Department of Chemistry and Biochemistry, Mendel University in Brno, Zemedelska 1, CZ-613 00 Brno, Czechia; Central European Institute of Technology, Brno University of Technology, Purkynova 123, CZ-612 00 Brno, Czechia.
authors.forename: Dagmar
authors.initials: D
authors.lastname: Hegerova
authors.affiliation: Department of Chemistry and Biochemistry, Mendel University in Brno, Zemedelska 1, CZ-613 00 Brno, Czechia; Central European Institute of Technology, Brno University of Technology, Purkynova 123, CZ-612 00 Brno, Czechia.
authors.forename: Ana Maria
authors.initials: AM
authors.lastname: Jimenez
authors.affiliation: Department of Chemistry and Biochemistry, Mendel University in Brno, Zemedelska 1, CZ-613 00 Brno, Czechia; Central European Institute of Technology, Brno University of Technology, Purkynova 123, CZ-612 00 Brno, Czechia.
authors.forename: Vedran
authors.initials: V
authors.lastname: Milosavljevic
authors.affiliation: Department of Chemistry and Biochemistry, Mendel University in Brno, Zemedelska 1, CZ-613 00 Brno, Czechia; Central European Institute of Technology, Brno University of Technology, Purkynova 123, CZ-612 00 Brno, Czechia.
authors.forename: Jiri
authors.initials: J
authors.lastname: Kudr
authors.affiliation: Department of Chemistry and Biochemistry, Mendel University in Brno, Zemedelska 1, CZ-613 00 Brno, Czechia; Central European Institute of Technology, Brno University of Technology, Purkynova 123, CZ-612 00 Brno, Czechia.
authors.forename: Sylvie
authors.initials: S
authors.lastname: Skalickova
authors.affiliation: Department of Chemistry and Biochemistry, Mendel University in Brno, Zemedelska 1, CZ-613 00 Brno, Czechia; Central European Institute of Technology, Brno University of Technology, Purkynova 123, CZ-612 00 Brno, Czechia.
authors.forename: David
authors.initials: D
authors.lastname: Hynek
authors.affiliation: Department of Chemistry and Biochemistry, Mendel University in Brno, Zemedelska 1, CZ-613 00 Brno, Czechia; Central European Institute of Technology, Brno University of Technology, Purkynova 123, CZ-612 00 Brno, Czechia.
authors.forename: Pavel
authors.initials: P
authors.lastname: Kopel
authors.affiliation: Department of Chemistry and Biochemistry, Mendel University in Brno, Zemedelska 1, CZ-613 00 Brno, Czechia; Central European Institute of Technology, Brno University of Technology, Purkynova 123, CZ-612 00 Brno, Czechia.
authors.forename: Marketa
authors.initials: M
authors.lastname: Vaculovicova
authors.affiliation: Department of Chemistry and Biochemistry, Mendel University in Brno, Zemedelska 1, CZ-613 00 Brno, Czechia; Central European Institute of Technology, Brno University of Technology, Purkynova 123, CZ-612 00 Brno, Czechia. Electronic address: vojtech.adam@mendelu.cz.
authors.forename: Vojtech
authors.initials: V
authors.lastname: Adam
mesh_terms: D058893:DNA Barcoding, Taxonomic / Q000379:methods*; D006801:Humans; D007711:Klebsiella pneumoniae / Q000737:chemistry* / Q000235:genetics; D008826:Microbial Sensitivity Tests / Q000379:methods; D045663:Quantum Dots / Q000737:chemistry*; D013211:Staphylococcus aureus / Q000737:chemistry* / Q000235:genetics
keywords: Barcode; Klebsiella pneumoniae; Magnetic particles; Methicillin-resistant Staphylococcus aureus; Quantum dots; Staphylococcus aureus