title: The utility of direct specimen detection by Sanger sequencing in hospitalized pediatric patients.
abstract: Direct microbial DNA detection from clinical specimens by polymerase chain reaction and Sanger sequencing has been developed to address the innate limitations of traditional culture-based work-up. We report our institution's experience with direct specimen sequencing, its clinical utility, and barriers to effective clinical implementation.
authors.affiliation: Division of Infectious Diseases, Department of Pediatrics, Children's Hospital Los Angeles, Los Angeles, CA 90027, USA; Keck School of Medicine at the University of Southern California, Los Angeles, CA 90033, USA. Electronic address: kmongkol@chla.usc.edu.
authors.forename: Kanokporn
authors.initials: K
authors.lastname: Mongkolrattanothai
authors.affiliation: Department of Pathology and Laboratory Medicine, Children's Hospital Los Angeles, Los Angeles, CA 90027, USA; Keck School of Medicine at the University of Southern California, Los Angeles, CA 90033, USA.
authors.forename: Jennifer
authors.initials: J
authors.lastname: Dien Bard
mesh_terms: D000293:Adolescent; D001424:Bacterial Infections / Q000175:diagnosis* / Q000382:microbiology; D002648:Child; D002675:Child, Preschool; D006801:Humans; D007223:Infant; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D009181:Mycoses / Q000175:diagnosis* / Q000382:microbiology; D016133:Polymerase Chain Reaction / Q000379:methods*; D017422:Sequence Analysis, DNA / Q000379:methods*
keywords: Direct specimen sequencing