title: Inhibition of Tobacco Ringspot Virus by the Culture Fluid of L-Lysine-α-Oxidase Producing Strain.
abstract: A producing strain of an anti-tumor and antiviral enzyme L-lysine-α-oxidase from Trichoderma was cultured using a technological device of G. K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences (Pushchino). L-lysine-α-oxidase activity in the obtained metabolite concentrate was 5.4 U/ml. We studied the effects of the concentrate of active L-lysine-α-oxidase producer on the highly infectious Tobacco ringspot virus and revealed anti-viral activity of it when enzyme concentration was at least 1.0 U/ml.
authors.affiliation: Medical Faculty, Medical Institute, Peoples' Friendship University of Russia, Moscow, Russia.
authors.forename: Yu A
authors.initials: YA
authors.lastname: Shneider
authors.affiliation: Medical Faculty, Medical Institute, Peoples' Friendship University of Russia, Moscow, Russia. smir-ip@yandex.ru.
authors.forename: I P
authors.initials: IP
authors.lastname: Smirnova
authors.affiliation: Medical Faculty, Medical Institute, Peoples' Friendship University of Russia, Moscow, Russia.
authors.forename: E V
authors.initials: EV
authors.lastname: Karimova
mesh_terms: D000594:Amino Acid Oxidoreductases / Q000096:biosynthesis / Q000302:isolation & purification / Q000494:pharmacology*; D000998:Antiviral Agents / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology*; D029068:Balsaminaceae / Q000821:virology; D003470:Culture Media / Q000737:chemistry; D005285:Fermentation; D005656:Fungal Proteins / Q000096:biosynthesis / Q000302:isolation & purification / Q000494:pharmacology*; D017860:Nepovirus / Q000187:drug effects* / Q000235:genetics / Q000254:growth & development; D012367:RNA, Viral / Q000037:antagonists & inhibitors* / Q000096:biosynthesis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014242:Trichoderma / Q000737:chemistry / Q000201:enzymology* / Q000254:growth & development
chemical_list: D000998:Antiviral Agents; D003470:Culture Media; D005656:Fungal Proteins; D012367:RNA, Viral; D000594:Amino Acid Oxidoreductases; C041123:L-lysine oxidase
keywords: Tobacco ringspot virus; Trichoderma culture fluid; antiviral enzyme L-lysine-α-oxidase