title: Simultaneous determination of glycyrrhizin and liquiritin in licorice roots and Kampo medicines by combination enzyme-linked immunosorbent assay using anti-glycyrrhizin and anti-liquiritin monoclonal antibodies.
abstract: Immunoassay systems using monoclonal antibodies (mAbs) are one of the most useful techniques in the analytical, biochemical, and clinical fields. In this study, a combination enzyme-linked immunosorbent assay (ELISA) using both anti-glycyrrhizin and anti-liquiritin mAbs (anti-GL/Liq mixture mAbs) was developed for quality control of licorice and its products. The combination ELISA demonstrated high sensitivity, reproducibility, and specificity for the total content of GL and Liq by a single assay. The developed ELISA was effective and useful as the first screening method in the selection of high-quality licorice from the Glycyrrhiza species and in confirming the quality of licorice-containing Kampo medicines.
authors.affiliation: a Department of Health and Nutrition, Faculty of Health Management , Nagasaki International University , Sasebo , Japan.
authors.forename: Shunsuke
authors.initials: S
authors.lastname: Fujii
authors.affiliation: b Department of Natural Medicines , Daiichi University of Pharmacy , Minami-ku , Fukuoka , Japan.
authors.forename: Osamu
authors.initials: O
authors.lastname: Morinaga
authors.affiliation: c Department of Pharmacognosy, Graduate School of Pharmaceutical Sciences , Nagasaki International University , Sasebo , Japan.
authors.forename: Takuhiro
authors.initials: T
authors.lastname: Uto
authors.affiliation: a Department of Health and Nutrition, Faculty of Health Management , Nagasaki International University , Sasebo , Japan.
authors.forename: Shuichi
authors.initials: S
authors.lastname: Nomura
authors.affiliation: c Department of Pharmacognosy, Graduate School of Pharmaceutical Sciences , Nagasaki International University , Sasebo , Japan.
authors.forename: Yukihiro
authors.initials: Y
authors.lastname: Shoyama
mesh_terms: D000911:Antibodies, Monoclonal / Q000276:immunology*; D000937:Antigen-Antibody Reactions; D004797:Enzyme-Linked Immunosorbent Assay; D044950:Flavanones / Q000032:analysis* / Q000276:immunology; D005960:Glucosides / Q000032:analysis* / Q000276:immunology; D006035:Glycyrrhiza / Q000737:chemistry*; D019695:Glycyrrhizic Acid / Q000032:analysis* / Q000276:immunology; D020835:Medicine, Kampo; D018517:Plant Roots / Q000737:chemistry*
chemical_list: D000911:Antibodies, Monoclonal; D044950:Flavanones; D005960:Glucosides; D019695:Glycyrrhizic Acid; C512196:liquiritin
keywords: Kampo medicines; combination ELISA; glycyrrhizin; licorice; liquiritin; simultaneous determination