title: Evolution of DS-1-like G1P[8] double-gene reassortant rotavirus A strains causing gastroenteritis in children in Vietnam in 2012/2013.
abstract: Rotavirus A (RVA) strains, a leading cause of severe gastroenteritis in children worldwide, commonly possess the Wa or DS-1 genotype constellations. During a hospital-based study conducted in Hanoi, Vietnam, in the 2012-2013 rotavirus season, G1P[8] strains with a virtually identical short RNA migration pattern were detected in 20 (14%) of 141 rotavirus-positive samples. Two representatives of these strains were shown by whole-genome sequencing to be double-gene reassortants possessing the genotype constellation of G1-P[8]-I2-R2-C2-M2-A2-N2-T2-E2-H2. Sequencing and a database search revealed that these Vietnamese G1P[8] double-gene reassortant strains shared an immediate ancestor with a locally circulating G2P[4] strain in all of the inner-capsid and non-structural protein genes, whereas they were more closely related in the VP7 and VP4 genes to a Chinese G1P[8] strain and a Chinese G3P[8] strain, respectively, than to locally circulating G1P[8] strains. Despite the marked similarity between Japanese and Thai G1P[8] double-gene reassortant strains, phylogenetic analysis suggested that the Vietnamese and Japanese/Thai G1P[8] double-gene reassortant strains originated from independent reassortment events. Clinically, children infected with Vietnamese G1P[8] double-gene reassortant strains experienced severe diarrhoea, but it was not more severe than that in children infected with ordinary G1P[8] strains. In conclusion, Vietnamese G1P[8] double-gene reassortant strains originated from a locally circulating G2P[4] strain and caused severe diarrhoea, but there was no evidence of increased virulence.
authors.affiliation: Department of Molecular Epidemiology, Graduate School of Biomedical Sciences, Nagasaki University, Nagasaki, 852-8523, Japan.
authors.forename: Toyoko
authors.initials: T
authors.lastname: Nakagomi
authors.affiliation: Department of Molecular Epidemiology, Graduate School of Biomedical Sciences, Nagasaki University, Nagasaki, 852-8523, Japan.|Department of Epidemiology, National Institute of Hygiene and Epidemiology, Hanoi, Vietnam.
authors.forename: Minh Quang
authors.initials: MQ
authors.lastname: Nguyen
authors.affiliation: Department of Molecular Epidemiology, Graduate School of Biomedical Sciences, Nagasaki University, Nagasaki, 852-8523, Japan.
authors.forename: Punita
authors.initials: P
authors.lastname: Gauchan
authors.affiliation: Department of Molecular Epidemiology, Graduate School of Biomedical Sciences, Nagasaki University, Nagasaki, 852-8523, Japan.
authors.forename: Chantal Ama
authors.initials: CA
authors.lastname: Agbemabiese
authors.affiliation: Department of Molecular Epidemiology, Graduate School of Biomedical Sciences, Nagasaki University, Nagasaki, 852-8523, Japan.
authors.forename: Miho
authors.initials: M
authors.lastname: Kaneko
authors.affiliation: Department of Molecular Epidemiology, Graduate School of Biomedical Sciences, Nagasaki University, Nagasaki, 852-8523, Japan.|Department of Virology, National Institute of Hygiene and Epidemiology, Hanoi, Vietnam.
authors.forename: Loan Phuong
authors.initials: LP
authors.lastname: Do
authors.affiliation: Department of Epidemiology, National Institute of Hygiene and Epidemiology, Hanoi, Vietnam.
authors.forename: Thiem Dinh
authors.initials: TD
authors.lastname: Vu
authors.affiliation: Department of Molecular Epidemiology, Graduate School of Biomedical Sciences, Nagasaki University, Nagasaki, 852-8523, Japan. onakagom@nagasaki-u.ac.jp.
authors.forename: Osamu
authors.initials: O
authors.lastname: Nakagomi
mesh_terms: D002648:Child; D002675:Child, Preschool; D003430:Cross-Sectional Studies; D019143:Evolution, Molecular; D005243:Feces / Q000821:virology; D005260:Female; D005759:Gastroenteritis / Q000453:epidemiology / Q000821:virology*; D016679:Genome, Viral; D005838:Genotype; D006801:Humans; D008297:Male; D010802:Phylogeny; D016865:Reassortant Viruses / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification / Q000502:physiology; D011995:Recombination, Genetic; D012401:Rotavirus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification / Q000502:physiology; D012400:Rotavirus Infections / Q000453:epidemiology / Q000821:virology*; D014744:Vietnam / Q000453:epidemiology