title: MicroRNA-150 upregulation reduces osteosarcoma cell invasion and metastasis by downregulating Ezrin.
abstract: The present study aimed to investigate the effect of microRNA-150 (miRNA/miR-150) in osteosarcoma (OS) cell invasion and metastasis by the regulation of Ezrin. To compare the differences in the expression of miR-150 and Ezrin, cell models of OS metastasis were established by exogenous transfection of miR-150 on the basis of different expression levels of miR-150. Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) was used to estimate these expression levels. Ezrin expression was detected by western blot assay. Methylthiazolyldiphenyl-tetrazolium bromide assay was performed to determine cells proliferation. Cell invasion and migration were measuredin vitroby Transwell migration assays. Detection of apoptosis adopted flow cytometry. The results of RT-qPCR showed that the miR-150 expression in OS F5M2 cells was significantly increased following exogenous transfection of miR-150 mimics, and the expression of miR-150 was positively correlated with the concentration of the miR-150 mimics. Western blot assay indicated that the Ezrin expression in the F5M2 cells was decreased with the exogenous overexpression of miR-150. Additionally, Transwell assays revealed that the overexpression of miR-150 significantly suppressed the invasion and metastasis ability of the F5M2 cells. miR-150 upregulation may reduce OS cell invasion and metastasis by downregulating the expression of Ezrin.
authors.affiliation: Department of Orthopedics, Changhai Hospital, The Second Military Medical University, Shanghai 200433, P.R. China.
authors.forename: Ce
authors.initials: C
authors.lastname: Zhan
authors.affiliation: Department of Orthopedics, Changhai Hospital, The Second Military Medical University, Shanghai 200433, P.R. China.
authors.forename: Cheng
authors.initials: C
authors.lastname: Li
authors.affiliation: Department of Orthopedics, Changhai Hospital, The Second Military Medical University, Shanghai 200433, P.R. China.
authors.forename: Hao
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Department of Orthopedics, Changhai Hospital, The Second Military Medical University, Shanghai 200433, P.R. China.
authors.forename: Hao
authors.initials: H
authors.lastname: Tang
authors.affiliation: Department of Orthopedics, Changhai Hospital, The Second Military Medical University, Shanghai 200433, P.R. China.
authors.forename: Fang
authors.initials: F
authors.lastname: Ji
authors.affiliation: Department of Orthopedics, Changhai Hospital, The Second Military Medical University, Shanghai 200433, P.R. China.
authors.forename: Su-Chi
authors.initials: SC
authors.lastname: Qiao
authors.affiliation: Department of Orthopedics, Changhai Hospital, The Second Military Medical University, Shanghai 200433, P.R. China.
authors.forename: Wei-Dong
authors.initials: WD
authors.lastname: Xu
authors.affiliation: Department of Orthopedics, Changhai Hospital, The Second Military Medical University, Shanghai 200433, P.R. China.
authors.forename: Zhi-Wei
authors.initials: ZW
authors.lastname: Wang
keywords: invasion and metastasis; microRNA-150; osteosarcoma