title: Folic acid conjugated and Ag-carrying organoclay nanoparticles and their response to L929 fibroblast and DLD-1 cancer cells.
abstract: This work presents the synthesis and characterisation of intercalated nanocomposites (NCs) from dispersed water solution blends of octadecyl amine-montmorillonite (ODA-MMT) (NC-0), folic acid (FA) conjugated ODA-MMT (NC-1) and Ag-MMT clay as a stable silver carrying agent (NC-2). The composition, chemical/physical and morphology of NCs with in situ intercalating nanostructures were investigated. Effect of organoclay, FA and Ag-MMT on L929 fibroblast (control), human colon carcinoma (DLD-1) cell lines, and the cytotoxicity, apoptosis and necrosis degree were estimated via WST-1/hemocytometric, double staining (as a ribonuclease A enzyme based method) and fluorescence microscopy methods in a dose-dependent manner. The mentioned cell lines integrated with NCs resulted in remarkable change in both morphology and nuclei of DLD-1 and fibroblast cells by apoptosis analysis. The number of necrotic cells were remarkably increased, as the toxic effects of nanocomposite nanoparticles were applied to both cell lines. Finally, the molecular mechanism of anticancer action of functionalised organoclays was elucidated.
authors.affiliation: a Institute of Science and Engineering, Division of Nanotechnology and Nanomedicine , Hacettepe University, Beytepe , Ankara , Turkey.
authors.forename: Zakir M O
authors.initials: ZM
authors.lastname: Rzayev
authors.affiliation: b Ministry of Public Health , Scientific Research Institute of Medicinal Prophylaxis , Baku-Sumgait , Azerbaijan.
authors.forename: Sevda
authors.initials: S
authors.lastname: Khalilova
authors.affiliation: c Department of Chemical Engineering, Faculty of Engineering , Hacettepe University, Beytepe Campus , Ankara , Turkey.
authors.forename: Kouroush
authors.initials: K
authors.lastname: Salimi
mesh_terms: D000970:Antineoplastic Agents / Q000008:administration & dosage* / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects; D001546:Bentonite / Q000737:chemistry*; D045744:Cell Line, Tumor; D003106:Colon / Q000187:drug effects / Q000473:pathology; D003110:Colonic Neoplasms / Q000188:drug therapy / Q000473:pathology; D004337:Drug Carriers / Q000737:chemistry*; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects; D005492:Folic Acid / Q000737:chemistry*; D006801:Humans; D053758:Nanoparticles / Q000737:chemistry*; D012834:Silver / Q000008:administration & dosage* / Q000494:pharmacology
chemical_list: D000970:Antineoplastic Agents; D004337:Drug Carriers; D001546:Bentonite; D012834:Silver; D005492:Folic Acid
keywords: Ag-MMT; DLD-1 cancer cell line; Folic acid; L929 fibroblast cells; anticancer activity; intercalating; nanocomposites; organoclay