title: Vaccination mitigates the impact of PRRSv infection on the pharmacokinetics of ceftiofur crystalline-free acid in pigs.
abstract: The pharmacokinetics of intramuscularly administered ceftiofur crystalline-free acid (CCFA) were determined in pigs that were clinically healthy (n = 8), vaccinated with a Porcine reproductive and respiratory syndrome modified live virus (PRRS MLV) (n = 10), challenged with wild-type porcine reproductive and respiratory syndrome virus (PRRSv) VR-2385 (n = 10), or vaccinated with PRRS MLV and later challenged with wild-type PRRSv VR-2385 (n = 10). Animals were given a single dose of CCFA intramuscularly at 5 mg/kg body weight. Blood was collected at 0 (pretreatment), 0.25, 0.5, 1, 6, 12, 24, 48, 96, 144, 192, and 240 h postinjection. Plasma was analyzed using liquid chromatography-mass spectrometry. Plasma concentration-time curves for each group were evaluated with noncompartmental modeling. When compared to control animals, those receiving the PRRSv wild-type challenge only had a lower AUC0-last, higher Cl/F, and higher Vz/F. The PRRSv wild-type challenge only group had the longest T1/2λ. The Cmaxdid not differ among all four treatments. Control animals had no statistically significant differences from animals vaccinated with PRRS MLV alone or animals vaccinated with PRRS MLV and later challenged with wild-type PRRSv. Our results suggest that PRRSv wild-type infection has the potential to alter CCFA pharmacokinetics and PRRS MLV vaccination may attenuate those changes.
authors.affiliation: Swine Medicine Education Center, College of Veterinary Medicine, Iowa State University, Ames, IA, USA.
authors.forename: J W
authors.initials: JW
authors.lastname: Sparks
authors.affiliation: Swine Medicine Education Center, College of Veterinary Medicine, Iowa State University, Ames, IA, USA.
authors.forename: L A
authors.initials: LA
authors.lastname: Karriker
authors.affiliation: Swine Medicine Education Center, College of Veterinary Medicine, Iowa State University, Ames, IA, USA.
authors.forename: D N
authors.initials: DN
authors.lastname: Day
authors.affiliation: Pharmacology Analytical Support Team (PhAST), College of Veterinary Medicine, Iowa State University, Ames, IA, USA.
authors.forename: L W
authors.initials: LW
authors.lastname: Wulf
authors.affiliation: Veterinary Diagnostic and Production Animal Medicine, Iowa State University, Ames, IA, USA.
authors.forename: J
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Pharmacology Analytical Support Team (PhAST), College of Veterinary Medicine, Iowa State University, Ames, IA, USA.
authors.forename: M L
authors.initials: ML
authors.lastname: Stock
authors.affiliation: Swine Medicine Education Center, College of Veterinary Medicine, Iowa State University, Ames, IA, USA.
authors.forename: J L
authors.initials: JL
authors.lastname: Bates
authors.affiliation: Department of Anatomy and Physiology, College of Veterinary Medicine, Kansas State University, Manhattan, KS, USA.
authors.forename: R
authors.initials: R
authors.lastname: Gehring
authors.affiliation: Pharmacology Analytical Support Team (PhAST), College of Veterinary Medicine, Iowa State University, Ames, IA, USA.|Veterinary Diagnostic and Production Animal Medicine, Iowa State University, Ames, IA, USA.
authors.forename: J F
authors.initials: JF
authors.lastname: Coetzee
mesh_terms: D000818:Animals; D002511:Cephalosporins / Q000493:pharmacokinetics*; D007273:Injections, Intramuscular / Q000662:veterinary; D019318:Porcine Reproductive and Respiratory Syndrome / Q000276:immunology*; D019316:Porcine respiratory and reproductive syndrome virus; D013552:Swine; D013553:Swine Diseases / Q000517:prevention & control*; D014611:Vaccination / Q000662:veterinary*
chemical_list: D002511:Cephalosporins; C053503:ceftiofur