title: Ectopic expression of GA 2-oxidase 6 from rapeseed (Brassica napus L.) causes dwarfism, late flowering and enhanced chlorophyll accumulation in Arabidopsis thaliana.
abstract: Gibberellins (GAs) are endogenous hormones that play an important role in higher plant growth and development. GA2-oxidase (GA2ox) promotes catabolism and inactivation of bioactive GAs or their precursors. In this study, we identified the GA2-oxidase gene, BnGA2ox6, and found it to be highly expressed in the silique and flower. Overexpression of BnGA2ox6 in Arabidopsis resulted in GA-deficiency symptoms, including inhibited elongation of the hypocotyl and stem, delayed seed germination, and late flowering. BnGA2ox6 overexpression reduced silique growth, but had no effect on seed development. Additionally, BnGA2ox6 overexpression enhanced chlorophyll b and total chlorophyll accumulation, and downregulated mRNA expression levels of the CHL1 and RCCR genes, which are involved in the chlorophyll degradation. These findings suggest that BnGA2ox6 regulates plant hight, silique development, flowering and chlorophyll accumulation in transgenic Arabidopsis.
authors.affiliation: Hunan Province Key Laboratory of Plant Functional Genomics and Developmental Regulation, College of Biology, Hunan University, Changsha, 410082, China.
authors.forename: Jindong
authors.initials: J
authors.lastname: Yan
authors.affiliation: Hunan Province Key Laboratory of Plant Functional Genomics and Developmental Regulation, College of Biology, Hunan University, Changsha, 410082, China.
authors.forename: Xiaoying
authors.initials: X
authors.lastname: Liao
authors.affiliation: Hunan Province Key Laboratory of Plant Functional Genomics and Developmental Regulation, College of Biology, Hunan University, Changsha, 410082, China.
authors.forename: Reqing
authors.initials: R
authors.lastname: He
authors.affiliation: Hunan Province Key Laboratory of Plant Functional Genomics and Developmental Regulation, College of Biology, Hunan University, Changsha, 410082, China.
authors.forename: Ming
authors.initials: M
authors.lastname: Zhong
authors.affiliation: Hunan Province Key Laboratory of Plant Functional Genomics and Developmental Regulation, College of Biology, Hunan University, Changsha, 410082, China.
authors.forename: Panpan
authors.initials: P
authors.lastname: Feng
authors.affiliation: Hunan Province Key Laboratory of Plant Functional Genomics and Developmental Regulation, College of Biology, Hunan University, Changsha, 410082, China.
authors.forename: Xinmei
authors.initials: X
authors.lastname: Li
authors.affiliation: Hunan Province Key Laboratory of Plant Functional Genomics and Developmental Regulation, College of Biology, Hunan University, Changsha, 410082, China.
authors.forename: Dongying
authors.initials: D
authors.lastname: Tang
authors.affiliation: Hunan Province Key Laboratory of Plant Functional Genomics and Developmental Regulation, College of Biology, Hunan University, Changsha, 410082, China; State Key Laboratory of Chemo/Biosensing and Chemometrics, Hunan University, Changsha, 410082, China. Electronic address: xmL05@hnu.edu.cn.
authors.forename: Xuanming
authors.initials: X
authors.lastname: Liu
authors.affiliation: Hunan Province Key Laboratory of Plant Functional Genomics and Developmental Regulation, College of Biology, Hunan University, Changsha, 410082, China. Electronic address: zxy_mm@163.com.
authors.forename: Xiaoying
authors.initials: X
authors.lastname: Zhao
mesh_terms: D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000033:anatomy & histology* / Q000235:genetics / Q000502:physiology*; D029688:Brassica napus / Q000201:enzymology*; D002734:Chlorophyll / Q000378:metabolism*; D035264:Flowers / Q000235:genetics / Q000502:physiology*; D018506:Gene Expression Regulation, Plant; D018525:Germination / Q000235:genetics; D018546:Hypocotyl / Q000254:growth & development; D006899:Mixed Function Oxygenases / Q000235:genetics / Q000378:metabolism*; D009154:Mutation / Q000235:genetics; D010641:Phenotype; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D018547:Plant Stems / Q000254:growth & development; D030821:Plants, Genetically Modified; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012639:Seeds / Q000235:genetics; D020539:Sequence Analysis, Protein
chemical_list: D010940:Plant Proteins; D012333:RNA, Messenger; D002734:Chlorophyll; D006899:Mixed Function Oxygenases
keywords: Arabidopsis thaliana; BnGA2ox6; Brassica napus L.; Chlorophyll; Dwarfism; Flowering