title: Impact of the Chronic Omega-3 Fatty Acids Supplementation in Hemiparkinsonism Model Induced by 6-Hydroxydopamine in Rats.
abstract: Parkinson's disease (PD) is characterized by a progressive degeneration of dopaminergic neurons in the substantia nigra. The neuronal degeneration may result from the convergence of a number of different pathogenic factors, including apoptosis, excitotoxicity and oxidative stress. Many studies emphasize the importance of omega-3 polyunsaturated fatty acids (ω-3 PUFAs) in vital processes such as maintenance of the properties of cell membranes and the participation in signal transduction and biodynamic activity of neuronal membranes. In this study, the protective effect of ω-3 PUFA administration on the 6-hydroxydopamine (6-OHDA) model of PD in rats was investigated. ω-3 PUFA (1.5 and 3.0 g/kg) was orally administered by gavage during 28 consecutive days to male Wistar rats. On the 4th day, hemiparkinsonism was induced through intrastriatal injection of 6-OHDA. On the 25th day, the animals were submitted to behavioural analysis. On the 28th day, after euthanasia, the brain areas were collected for neurochemical evaluation. ω-3 PUFAs (1.5 and 3.0 g/kg) restored monoamine and amino acid levels on the striatum from hemiparkinsonian rats, followed by reduction in the number of apomorphine-induced rotations and promotion of a partial locomotor recovery. In addition, ω-3 PUFAs (1.5 and 3.0 g/kg) decreased the lipid peroxidation levels and nitrite levels in the brain areas from hemiparkinsonian rats. Thus, this study suggests that supplementation with ω-3 PUFAs prevents behavioural and neurochemical disturbances induced by 6-OHDA, presenting a potential neuroprotective action.
authors.affiliation: Faculty of Medicine, Federal University of Ceará, Sobral, Ceará, Brazil.
authors.forename: Alexandre Sales
authors.initials: AS
authors.lastname: Barros
authors.affiliation: Faculty of Medicine, Federal University of Ceará, Sobral, Ceará, Brazil.
authors.forename: Rafael Yuri Gouveia
authors.initials: RYG
authors.lastname: Crispim
authors.affiliation: Faculty of Medicine, Federal University of Ceará, Sobral, Ceará, Brazil.
authors.forename: Juliana Uchoa
authors.initials: JU
authors.lastname: Cavalcanti
authors.affiliation: Department of Biochemistry and Molecular Biology, Federal University of Ceará, Fortaleza, Ceará, Brazil.
authors.forename: Ricardo Basto
authors.initials: RB
authors.lastname: Souza
authors.affiliation: Faculty of Medicine, Federal University of Ceará, Sobral, Ceará, Brazil.
authors.forename: Jonatas Cavalcante
authors.initials: JC
authors.lastname: Lemos
authors.affiliation: Faculty of Medicine, Federal University of Ceará, Sobral, Ceará, Brazil.
authors.forename: Gerardo
authors.initials: G
authors.lastname: Cristino Filho
authors.affiliation: Faculty of Medicine, Federal University of Ceará, Sobral, Ceará, Brazil.
authors.forename: Mirna Marques
authors.initials: MM
authors.lastname: Bezerra
authors.affiliation: Faculty of Medicine, Federal University of Ceará, Sobral, Ceará, Brazil.
authors.forename: Thales Fontenele Moraes
authors.initials: TFM
authors.lastname: Pinheiro
authors.affiliation: Department of Physiology and Pharmacology, Federal University of Ceará, Fortaleza, Ceará, Brazil.
authors.forename: Silvânia Maria Mendes
authors.initials: SMM
authors.lastname: de Vasconcelos
authors.affiliation: Department of Physiology and Pharmacology, Federal University of Ceará, Fortaleza, Ceará, Brazil.
authors.forename: Danielle Silveira
authors.initials: DS
authors.lastname: Macêdo
authors.affiliation: Department of Physiology and Pharmacology, Federal University of Ceará, Fortaleza, Ceará, Brazil.
authors.forename: Glauce Socorro
authors.initials: GS
authors.lastname: de Barros Viana
authors.affiliation: Faculty of Medicine, Federal University of Ceará, Sobral, Ceará, Brazil.
authors.forename: Lissiana Magna Vasconcelos
authors.initials: LMV
authors.lastname: Aguiar
mesh_terms: D000818:Animals; D001058:Apomorphine / Q000494:pharmacology; D015306:Biogenic Monoamines / Q000032:analysis; D001921:Brain / Q000187:drug effects / Q000378:metabolism; D019587:Dietary Supplements; D004195:Disease Models, Animal; D015525:Fatty Acids, Omega-3 / Q000008:administration & dosage*; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D009043:Motor Activity / Q000187:drug effects; D009573:Nitrites / Q000032:analysis; D016627:Oxidopamine; D010300:Parkinson Disease / Q000378:metabolism / Q000517:prevention & control*; D051381:Rats; D017208:Rats, Wistar; D005680:gamma-Aminobutyric Acid / Q000032:analysis
chemical_list: D015306:Biogenic Monoamines; D015525:Fatty Acids, Omega-3; D009573:Nitrites; D005680:gamma-Aminobutyric Acid; D016627:Oxidopamine; D001058:Apomorphine