title: Silencing of the long non-coding RNA NEAT1 suppresses glioma stem-like properties through modulation of the miR-107/CDK6 pathway.
abstract: Developing novel strategies against glioma remains a significant challenge. Long non-coding RNA nuclear paraspeckle assembly transcript 1 (NEAT1) significantly contributes to the progression of many human cancers, while glioma stem cells (GSCs) are believed to be responsible for tumor progression. However, whether NEAT1 alters the stem-like properties of GSC cells remains unknown. Using microbeads, CD133+ cells were isolated and were found to possess glioma stem cell properties. Using siRNA, NEAT1 was knocked down in the sorted CD133+ U87 glioma cells. We found higher NEAT1 RNA expression in CD133+ human glioma primary culture stem cells and CD133+ U87 cells via RT-PCR. Moreover, NEAT1 knockdown in the CD133+ U87 cells resulted in decreased colony formation, increased G1 cell cycle arrest and apoptosis. In addition, these effects were accompanied by miR-107 activation and inactivation of CDK6 protein. These results strongly suggest that NEAT1 knockdown suppresses stem-like properties in glioma cells by modulating the miR‑107CDK6 pathway. Targeting NEAT1 may provide a novel therapeutic opportunity for developing a relapse-free treatment of glioma patients.
authors.affiliation: Department of Neurology, The First Affiliated Hospital, and College of Clinical Medicine of Henan University of Science and Technology, Luoyang, Henan 471003, P.R. China.
authors.forename: Xiaoli
authors.initials: X
authors.lastname: Yang
authors.affiliation: Department of Neurosurgery, The First Affiliated Hospital, and College of Clinical Medicine of Henan University of Science and Technology, Luoyang, Henan 471003, P.R. China.
authors.forename: Zheng
authors.initials: Z
authors.lastname: Xiao
authors.affiliation: Department of Neurology, The First Affiliated Hospital, and College of Clinical Medicine of Henan University of Science and Technology, Luoyang, Henan 471003, P.R. China.
authors.forename: Xiaowei
authors.initials: X
authors.lastname: Du
authors.affiliation: Department of Neurology, The First Affiliated Hospital, and College of Clinical Medicine of Henan University of Science and Technology, Luoyang, Henan 471003, P.R. China.
authors.forename: Lina
authors.initials: L
authors.lastname: Huang
authors.affiliation: Department of Neurology, The First Affiliated Hospital, and College of Clinical Medicine of Henan University of Science and Technology, Luoyang, Henan 471003, P.R. China.
authors.forename: Ganqin
authors.initials: G
authors.lastname: Du
mesh_terms: D000368:Aged; D045744:Cell Line, Tumor; D051361:Cyclin-Dependent Kinase 6 / Q000235:genetics* / Q000378:metabolism; D005260:Female; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D055785:Gene Knockdown Techniques; D005910:Glioma / Q000235:genetics / Q000473:pathology*; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D008875:Middle Aged; D014411:Neoplastic Stem Cells / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D034622:RNA Interference; D062085:RNA, Long Noncoding / Q000037:antagonists & inhibitors* / Q000235:genetics; D034741:RNA, Small Interfering / Q000494:pharmacology*; D015398:Signal Transduction / Q000187:drug effects / Q000235:genetics
chemical_list: C549045:MIRN107 microRNA, human; D035683:MicroRNAs; C577276:NEAT1 long non-coding RNA, human; D062085:RNA, Long Noncoding; D034741:RNA, Small Interfering; C495911:CDK6 protein, human; D051361:Cyclin-Dependent Kinase 6