title: Biosynthesis, characterization, and evaluation of bioactivities of leaf extract-mediated biocompatible silver nanoparticles from an early tracheophyte,Pteris tripartitaSw.
abstract: The objective of the study was to characterize silver nanoparticles (Ag-NPs) and their bioactivities in early tracheophytes (Pteridophyta). Aqueous leaf extract of a critically endangered fern,Pteris tripartitaSw., was used for one-step green synthesis of Ag-NPs. The biosynthesized Ag-NPs were characterized using ultraviolet-visible spectroscopy, Fourier transform infrared spectroscopy, scanning electron microscopy, energy-dispersive X-ray spectroscopy, X-ray diffraction, and high-resolution transmission electron microscopy. Morphologically, the Ag-NPs showed hexagonal, spherical, and rod-shaped structures. Size distributions of Ag-NPs, calculated using Scherrer's formula, showed an average size of 32 nm. Ag-NPs were studied for in vitro antioxidant, antimicrobial, and in vivo anti-inflammatory activities. Ag-NPs exhibited significant anti-inflammatory activity in carrageenan-induced paw volume tests performed in female Wistar albino rats. Furthermore, Ag-NPs showed significant antimicrobial activity against 12 different microorganisms in three different assays (disk diffusion, time course growth, and minimum inhibitory concentration). This study reports that colloidal Ag-NPs can be synthesized by simple, nonhazardous methods, and that biosynthesized Ag-NPs have significant therapeutic properties.
authors.affiliation: Shenzhen Key Laboratory of Southern Subtropical Plant Diversity, Fairy Lake Botanical Garden, Shenzhen and Chinese Academy of Sciences, Shenzhen, People's Republic of China.
authors.forename: Xavierravi
authors.initials: X
authors.lastname: Baskaran
authors.affiliation: Department of Zoology, St Xavier's College, Palayamkottai.
authors.forename: Antony Varuvel
authors.initials: AV
authors.lastname: Geo Vigila
authors.affiliation: Department of Botany, Bioprospecting Laboratory, Bharathiar University, Coimbatore, Tamil Nadu.
authors.forename: Thangaraj
authors.initials: T
authors.lastname: Parimelazhagan
authors.affiliation: Department of Biotechnology, Sri Krishnadevaraya University, Anantapur, Andhra Pradesh, India.
authors.forename: Doulathabad
authors.initials: D
authors.lastname: Muralidhara-Rao
authors.affiliation: Shenzhen Key Laboratory of Southern Subtropical Plant Diversity, Fairy Lake Botanical Garden, Shenzhen and Chinese Academy of Sciences, Shenzhen, People's Republic of China.
authors.forename: Shouzhou
authors.initials: S
authors.lastname: Zhang
mesh_terms: D000818:Animals; D000890:Anti-Infective Agents / Q000737:chemistry / Q000494:pharmacology; D000893:Anti-Inflammatory Agents / Q000737:chemistry / Q000494:pharmacology; D005260:Female; D053768:Metal Nanoparticles; D008826:Microbial Sensitivity Tests; D010936:Plant Extracts / Q000737:chemistry*; D018515:Plant Leaves / Q000737:chemistry; D032495:Pteris / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D012834:Silver / Q000737:chemistry* / Q000494:pharmacology*
chemical_list: D000890:Anti-Infective Agents; D000893:Anti-Inflammatory Agents; D010936:Plant Extracts; D012834:Silver
keywords: FTIR; HRTEM; Pteris tripartita; antimicrobial; antioxidant; silver nanoparticles