title: miRNAs differentially expressed by next-generation sequencing in cord blood buffy coat samples of boys and girls.
abstract: Differences in children's development and susceptibility to diseases and exposures have been observed by sex, yet human studies of sex differences in miRNAs are limited.
  
  
  
  The genome-wide miRNA expression was characterized by sequencing-based EdgeSeq assay in cord blood buffy coats from 89 newborns, and 564 miRNAs were further analyzed.
  
  
  
  Differential expression of most miRNAs was higher in boys. Neurodevelopment, RNA metabolism and metabolic ontology terms were enriched among miRNA targets. The majority of upregulated miRNAs (86%) validated by nCounter maintained positive-fold change values; however, only 21% reached statistical significance by false discovery rate.
  
  
  
  Accounting for host factors like sex may improve the sensitivity of epigenetic analyses for epidemiological studies in early childhood.
authors.affiliation: School of Public Health, Center for Environmental Research on Children's Health (CERCH), University of California, Berkeley, CA 94720, USA.
authors.forename: Daneida
authors.initials: D
authors.lastname: Lizarraga
authors.affiliation: School of Public Health, Center for Environmental Research on Children's Health (CERCH), University of California, Berkeley, CA 94720, USA.
authors.forename: Karen
authors.initials: K
authors.lastname: Huen
authors.affiliation: School of Public Health, Center for Environmental Research on Children's Health (CERCH), University of California, Berkeley, CA 94720, USA.
authors.forename: Mary
authors.initials: M
authors.lastname: Combs
authors.affiliation: School of Public Health, Center for Environmental Research on Children's Health (CERCH), University of California, Berkeley, CA 94720, USA.
authors.forename: Maria
authors.initials: M
authors.lastname: Escudero-Fung
authors.affiliation: School of Public Health, Center for Environmental Research on Children's Health (CERCH), University of California, Berkeley, CA 94720, USA.
authors.forename: Brenda
authors.initials: B
authors.lastname: Eskenazi
authors.affiliation: School of Public Health, Center for Environmental Research on Children's Health (CERCH), University of California, Berkeley, CA 94720, USA.
authors.forename: Nina
authors.initials: N
authors.lastname: Holland
mesh_terms: D058621:Blood Buffy Coat / Q000378:metabolism; D005260:Female; D005312:Fetal Blood / Q000378:metabolism; D006801:Humans; D007231:Infant, Newborn; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D017423:Sequence Analysis, RNA; D012737:Sex Factors
chemical_list: D035683:MicroRNAs
keywords: cord blood; early life; epigenetics; filtering; miRNA; miRNAome; newborns; next-generation sequencing; normalization procedures; sex differences