title: Data set for describing the elaboration of a compatible Gateway-based co-expression vector set and supporting its validation.
abstract: This article contains Supplementary Data including methods and figures that relate to the article entitled "Construction of a compatible Gateway-based co-expression vector set for expressing multiprotein complexes inE. coli" (L. Salim, C. Feger, D. Busso, 2016) [1] that describes the elaboration and the validation of a set of versatile compatible plasmids for co-expression studies inEscherichia coli. Here, we describe experimental procedures for plasmid construction and recombinant protein expression. We give the list of the 33 (co)-expression plasmids encoding fluorescent protein and we show extensive experimental data obtained for all combinations tested for validating our vector set.
authors.affiliation: Service de Biologie Moléculaire, Institut de Génétique et de Biologie Moléculaire et Cellulaire, CNRS/Inserm/Université de Strasbourg, 1 rue Laurent Fries, BP10142, 67404 Illkirch cedex, France.
authors.forename: Loubna
authors.initials: L
authors.lastname: Salim
authors.affiliation: Service de Biologie Moléculaire, Institut de Génétique et de Biologie Moléculaire et Cellulaire, CNRS/Inserm/Université de Strasbourg, 1 rue Laurent Fries, BP10142, 67404 Illkirch cedex, France.
authors.forename: Claire
authors.initials: C
authors.lastname: Feger
authors.affiliation: Service de Biologie Moléculaire, Institut de Génétique et de Biologie Moléculaire et Cellulaire, CNRS/Inserm/Université de Strasbourg, 1 rue Laurent Fries, BP10142, 67404 Illkirch cedex, France.
authors.forename: Didier
authors.initials: D
authors.lastname: Busso
keywords: Co-expression; Expression screening; Gateway; Protein complex; Recombinational cloning; Versatility