title: Valveless pumping mechanics of the embryonic heart during cardiac looping: Pressure and flow through micro-PIV.
abstract: Cardiovascular development is influenced by the flow-induced stress environment originating from cardiac biomechanics. To characterize the stress environment, it is necessary to quantify flow and pressure. Here, we quantify the flow field in a developing zebrafish heart during the looping stage through micro-particle imaging velocimetry and by analyzing spatiotemporal plots. We further build upon previous methods to noninvasively quantify the pressure field at a low Reynolds number using flow field data for the first time, while also comparing the impact of viscosity models. Through this method, we show that the atrium builds up pressure to ~0.25mmHg relative to the ventricle during atrial systole and that atrial expansion creates a pressure difference of ~0.15mmHg across the atrium, resulting in efficient cardiac pumping. With these techniques, it is possible to noninvasively fully characterize hemodynamics during heart development.
authors.affiliation: Department of Mechanical Engineering, Colorado State University, 1374 Campus Delivery, Fort Collins, CO, USA; School of Biomedical Engineering, Colorado State University, Fort Collins, CO, USA.
authors.forename: D L
authors.initials: DL
authors.lastname: Bark
authors.affiliation: Department of Mechanical Engineering, Colorado State University, 1374 Campus Delivery, Fort Collins, CO, USA; School of Biomedical Engineering, Colorado State University, Fort Collins, CO, USA.
authors.forename: B
authors.initials: B
authors.lastname: Johnson
authors.affiliation: Department of Biology, Colorado State University, 1878 Campus Delivery, Fort Collins, CO, USA.
authors.forename: D
authors.initials: D
authors.lastname: Garrity
authors.affiliation: Department of Mechanical Engineering, Colorado State University, 1374 Campus Delivery, Fort Collins, CO, USA; School of Biomedical Engineering, Colorado State University, Fort Collins, CO, USA; Department of Biomedical Engineering and Surgery, The Ohio State University, 473 W 12th Ave., DHLRI, Columbus, OH, USA. Electronic address: dasi.1@osu.edu.
authors.forename: L P
authors.initials: LP
authors.lastname: Dasi
mesh_terms: D000818:Animals; D062185:Atrial Pressure; D004625:Embryo, Nonmammalian; D047108:Embryonic Development / Q000502:physiology*; D006321:Heart / Q000196:embryology* / Q000502:physiology*; D006439:Hemodynamics; D012212:Rheology; D013599:Systole; D017725:Ventricular Pressure; D014783:Viscosity; D015027:Zebrafish
keywords: Blood flow; Development; Heart; Pressure; Zebrafish