title: Production, Characterization and Application of a Thermostable Tannase fromPestalotiopsis guepiniiURM 7114.
abstract: Tannase (EC 3.1.1.20) is an enzyme that hydrolyzes the ester and depside bonds of tannic acid to gallic acid and glucose. In the production of foods and beverages, it contributes to the removal of the undesirable effects of tannins. The aim of this study is to investigate the potential of endophytic fungi isolated from jamun (Syzygium cumini(L.) Skeels) leaves, and identified asPestalotiopsis guepinii, in the production of tannase. Tannase was produced extracellularly byP. guepiniiunder submerged, slurry-state and solid-state fermentations. The submerged fermentation was found to be the most promising (98.6 U/mL). Response surface methodology was employed to evaluate the effect of variables (pH and temperature), and the results showed that the best conditions for tannase activity were pH=6.9 and 30 °C.Kmwas found to be 7.18·10-4mol/L andvmax=250.00 U/mL. The tannase activity was the highest in the presence of Ca2+at a concentration of 5·10-3mol/L. Moreover, the enzyme was not inhibited by the tested chelators and detergents. The stability of the enzyme was also studied, and crude enzyme was evaluated in simulation of gastrointestinal digestion of monogastric animals. The crude enzyme was highly stable under simulated conditions; it retained 87.3% of its original activity after 6 h. The study contributes to the identification of microbial species that produce tannase, with potential application in biotechnology.
authors.affiliation: Microbiology Laboratory, Federal Institute of Education, Science and Technology of Pernambuco, Campus Barreiros, 55560-000 Barreiros, PE, Brazil; Laboratory of Enzymology, Department of Health, State University of Feira de Santana, 44036-900 Feira de Santana, BA, Brazil.
authors.forename: Amanda
authors.initials: A
authors.lastname: Reges de Sena
authors.affiliation: Microbiology Laboratory, Federal Institute of Education, Science and Technology of Pernambuco, Campus Barreiros, 55560-000 Barreiros, PE, Brazil.
authors.forename: Ana
authors.initials: A
authors.lastname: Claúdia de Barros Dos Santos
authors.affiliation: Microbiology Laboratory, Federal Institute of Education, Science and Technology of Pernambuco, Campus Barreiros, 55560-000 Barreiros, PE, Brazil.
authors.forename: Miquéas Jamesse
authors.initials: MJ
authors.lastname: Gouveia
authors.affiliation: Microbiology Laboratory, Federal Institute of Education, Science and Technology of Pernambuco, Campus Barreiros, 55560-000 Barreiros, PE, Brazil.
authors.forename: Marcelo Rodrigues
authors.initials: MR
authors.lastname: Figueira de Mello
authors.affiliation: Bioassays Laboratory for Drug Research, Federal University of Pernambuco, 50670-420 Recife, PE, Brazil.
authors.forename: Tonny Cley Campos
authors.initials: TC
authors.lastname: Leite
authors.affiliation: Central Laboratory of Garanhuns, Laboratory of Biotechnology, Academic Unit of Garanhuns, Federal Rural University of Pernambuco, 55292-270 Garanhuns, PE, Brazil.
authors.forename: Keila Aparecida
authors.initials: KA
authors.lastname: Moreira
authors.affiliation: Laboratory of Enzymology, Department of Health, State University of Feira de Santana, 44036-900 Feira de Santana, BA, Brazil.
authors.forename: Sandra
authors.initials: S
authors.lastname: Aparecida de Assis
keywords: Pestalotiopsis guepinii; animal feed; endophytic fungus; submerged fermentation; tannin acyl hydrolase (tannase)