title: Sugaring-out extraction of acetoin from fermentation broth by coupling with fermentation.
abstract: Acetoin is a natural flavor and an important bio-based chemical which could be separated from fermentation broth by solvent extraction, salting-out extraction or recovered in the form of derivatives. In this work, a novel method named as sugaring-out extraction coupled with fermentation was tried in the acetoin production by Bacillus subtilis DL01. The effects of six solvents on bacterial growth and the distribution of acetoin and glucose in different solvent-glucose systems were explored. The operation parameters such as standing time, glucose concentration, and volume ratio of ethyl acetate to fermentation broth were determined. In a system composed of fermentation broth, glucose (100%, m/v) and two-fold volume of ethyl acetate, nearly 100% glucose was distributed into bottom phase, and 61.2% acetoin into top phase without coloring matters and organic acids. The top phase was treated by vacuum distillation to remove solvent and purify acetoin, while the bottom phase was used as carbon source to produce acetoin in the next batch of fermentation.
authors.affiliation: School of Life Science and Biotechnology, Dalian University of Technology, Dalian, 116024, People's Republic of China.
authors.forename: Jian-Ying
authors.initials: JY
authors.lastname: Dai
authors.affiliation: School of Life Science and Biotechnology, Dalian University of Technology, Dalian, 116024, People's Republic of China.
authors.forename: Lin-Hui
authors.initials: LH
authors.lastname: Ma
authors.affiliation: School of Life Science and Biotechnology, Dalian University of Technology, Dalian, 116024, People's Republic of China.
authors.forename: Zhuang-Fei
authors.initials: ZF
authors.lastname: Wang
authors.affiliation: School of Life Science and Biotechnology, Dalian University of Technology, Dalian, 116024, People's Republic of China.
authors.forename: Wen-Tian
authors.initials: WT
authors.lastname: Guan
authors.affiliation: School of Life Science and Biotechnology, Dalian University of Technology, Dalian, 116024, People's Republic of China. zhlxiu@dlut.edu.cn.
authors.forename: Zhi-Long
authors.initials: ZL
authors.lastname: Xiu
mesh_terms: D000085:Acetates / Q000737:chemistry; D000093:Acetoin / Q000737:chemistry*; D000143:Acids / Q000737:chemistry; D001412:Bacillus subtilis / Q000235:genetics / Q000378:metabolism*; D002072:Butylene Glycols / Q000737:chemistry; D002623:Chemistry Techniques, Analytical; D003116:Color; D003470:Culture Media / Q000737:chemistry; D005285:Fermentation; D005947:Glucose / Q000737:chemistry; D007218:Industrial Microbiology / Q000379:methods*; D060847:Metabolic Engineering; D012997:Solvents / Q000737:chemistry
chemical_list: D000085:Acetates; D000143:Acids; D002072:Butylene Glycols; D003470:Culture Media; D012997:Solvents; C007650:ethyl acetate; D000093:Acetoin; D005947:Glucose
keywords: Acetoin; Recovery coupled with fermentation; Solvent selection; Sugaring-out extraction