title: TGF-β signaling controls FSHR signaling-reduced ovarian granulosa cell apoptosis through the SMAD4/miR-143 axis.
abstract: Follicle-stimulating hormone receptor (FSHR) and its intracellular signaling control mammalian follicular development and female infertility. Our previous study showed that FSHR is downregulated during follicular atresia of porcine ovaries. However, its role and regulation in follicular atresia remain unclear. Here, we showed that FSHR knockdown induced porcine granulosa cell (pGC) apoptosis and follicular atresia, and attenuated the levels of intracellular signaling molecules such as PKA, AKT and p-AKT. FSHR was identified as a target of miR-143, a microRNA that was upregulated during porcine follicular atresia. miR-143 enhanced pGC apoptosis by targeting FSHR, and reduced the levels of intracellular signaling molecules. SMAD4, the final molecule in transforming growth factor (TGF)-β signaling, bound to the promoter and induced significant downregulation of miR-143 in vitro and in vivo. Activated TGF-β signaling rescued miR-143-reduced FSHR and intracellular signaling molecules, and miR-143-induced pGC apoptosis. Overall, our findings offer evidence to explain how TGF-β signaling influences and FSHR signaling for regulation of pGC apoptosis and follicular atresia by a specific microRNA, miR-143.
authors.affiliation: College of Animal Science and Technology, Department of Animal Genetics, Breeding and Reproduction, Nanjing Agricultural University, Nanjing, China.
authors.forename: Xing
authors.initials: X
authors.lastname: Du
authors.affiliation: College of Animal Science and Technology, Department of Animal Genetics, Breeding and Reproduction, Nanjing Agricultural University, Nanjing, China.
authors.forename: Lifan
authors.initials: L
authors.lastname: Zhang
authors.affiliation: College of Animal Science and Technology, Department of Animal Genetics, Breeding and Reproduction, Nanjing Agricultural University, Nanjing, China.
authors.forename: Xinyu
authors.initials: X
authors.lastname: Li
authors.affiliation: College of Animal Science and Technology, Department of Animal Genetics, Breeding and Reproduction, Nanjing Agricultural University, Nanjing, China.
authors.forename: Zengxiang
authors.initials: Z
authors.lastname: Pan
authors.affiliation: College of Animal Science and Technology, Department of Animal Genetics, Breeding and Reproduction, Nanjing Agricultural University, Nanjing, China.
authors.forename: Honglin
authors.initials: H
authors.lastname: Liu
authors.affiliation: College of Animal Science and Technology, Department of Animal Genetics, Breeding and Reproduction, Nanjing Agricultural University, Nanjing, China.
authors.forename: Qifa
authors.initials: Q
authors.lastname: Li
mesh_terms: D000818:Animals; D017209:Apoptosis; D001483:Base Sequence; D005260:Female; D005496:Follicular Atresia / Q000378:metabolism; D055785:Gene Knockdown Techniques; D006107:Granulosa Cells / Q000166:cytology* / Q000378:metabolism*; D035683:MicroRNAs / Q000378:metabolism*; D008954:Models, Biological; D011401:Promoter Regions, Genetic / Q000235:genetics; D011485:Protein Binding / Q000235:genetics; D011962:Receptors, FSH / Q000378:metabolism*; D015398:Signal Transduction; D051901:Smad4 Protein / Q000378:metabolism*; D034421:Sus scrofa; D016212:Transforming Growth Factor beta / Q000378:metabolism*
chemical_list: D035683:MicroRNAs; D011962:Receptors, FSH; D051901:Smad4 Protein; D016212:Transforming Growth Factor beta