title: Annulohpoxylotol A and B, new sesquiterpenoids from the endophytic fungus Annulohypoxylon truncatum, are natural NF-κB inhibitors.
abstract: Two sesquiterpenoids, annulohpoxylotol A and B, were isolated along with five sterols from an ethyl acetate extract of cultures of the endophytic fungus Annulohypoxylon truncatum growing on leaves of Zizania caduciflora. The structures of the isolated compounds were established using one-dimensional (1D) and two-dimensional (2D)-NMR and mass spectrometry. The nuclear factor-kappa B (NF-κB) inhibitory activities of the isolated compounds stimulated with tumor necrosis factor-alpha (TNF-α) were measured using a luciferase reporter system. Annulohpoxylotol A (1) significantly inhibited NF-κB activation in a dose-dependent manner, with an IC50of 7.11 μM, whereas annulohpoxylotol B (2) and ergone (7) moderately inhibited NF-κB transcriptional activity, with IC50values of 19.24 and 17.51 μM, respectively.
authors.affiliation: Korean Medicine (KM) Application Center, Korea Institute of Oriental Medicine, Daegu, 41062, Republic of Korea.
authors.forename: Wei
authors.initials: W
authors.lastname: Li
authors.affiliation: College of Pharmacy, Chungnam National University, Daejeon, 34134, Republic of Korea.
authors.forename: Ya Nan
authors.initials: YN
authors.lastname: Sun
authors.affiliation: College of Pharmacy, Duksung Women's University, Seoul, 01369, Republic of Korea.
authors.forename: Changyeol
authors.initials: C
authors.lastname: Lee
authors.affiliation: College of Pharmacy, Duksung Women's University, Seoul, 01369, Republic of Korea.
authors.forename: Sung Hee
authors.initials: SH
authors.lastname: Bang
authors.affiliation: National Institute of Biological Resources, Incheon, 22689, Republic of Korea.
authors.forename: Soonok
authors.initials: S
authors.lastname: Kim
authors.affiliation: Korean Medicine (KM) Application Center, Korea Institute of Oriental Medicine, Daegu, 41062, Republic of Korea. jyma@kiom.ac.kr.
authors.forename: Jin Yeul
authors.initials: JY
authors.lastname: Ma
authors.affiliation: College of Pharmacy, Chungnam National University, Daejeon, 34134, Republic of Korea. yhk@cnu.ac.kr.
authors.forename: Young Ho
authors.initials: YH
authors.lastname: Kim
authors.affiliation: College of Pharmacy, Duksung Women's University, Seoul, 01369, Republic of Korea. sangheeshim@duksung.ac.kr.
authors.forename: Sang Hee
authors.initials: SH
authors.lastname: Shim
mesh_terms: D001203:Ascomycota / Q000737:chemistry*; D002783:Cholestenones; D004875:Ergosterol / Q000031:analogs & derivatives* / Q000494:pharmacology; D006801:Humans; D020128:Inhibitory Concentration 50; D008156:Luciferases; D013058:Mass Spectrometry; D016328:NF-kappa B / Q000037:antagonists & inhibitors*; D019906:Nuclear Magnetic Resonance, Biomolecular; D006109:Poaceae / Q000382:microbiology; D012717:Sesquiterpenes / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D002783:Cholestenones; D016328:NF-kappa B; D012717:Sesquiterpenes; D014409:Tumor Necrosis Factor-alpha; C000616598:annulohpoxylotol A; C000616651:annulohpoxylotol B; D008156:Luciferases; C015860:ergosta-4,6,8(14),22-tetraen-3-one; D004875:Ergosterol
keywords: Annulohypoxylon truncatum; Endophytic fungus; NF-κB; Sesquiterpenoid