title: Self-assembling of poly(aspartic acid) with bovine serum albumin in aqueous solutions.
abstract: Macromolecular co-assemblies built up in aqueous solutions, by using a linear polypeptide, poly(aspartic acid) (PAS), and a globular protein, bovine serum albumin (BSA), have been studied. The main interest was to identify the optimum conditions for an interpenetrated complex formation in order to design materials suitable for biomedical applications, such as drug delivery systems. BSA surface possesses several amino- and carboxylic groups available for covalent modification, and/or bioactive substances attachment. In the present study, mixtures between PAS and BSA were investigated at 37°C in dilute aqueous solution by viscometry, dynamic light scattering and zeta potential determination, as well as in solid state by AFM microscopy and dielectric spectroscopy. The experimental data have shown that the interpolymer complex formation occurs for a PAS/BSA molar ratio around 0.541.
authors.affiliation: "Petru Poni" Institute of Macromolecular Chemistry, 41-A Grigore Ghica Voda Alley, RO-700487 Iasi, Romania. Electronic address: lnazarie@yahoo.co.uk.
authors.forename: L E
authors.initials: LE
authors.lastname: Nita
authors.affiliation: "Petru Poni" Institute of Macromolecular Chemistry, 41-A Grigore Ghica Voda Alley, RO-700487 Iasi, Romania.
authors.forename: A P
authors.initials: AP
authors.lastname: Chiriac
authors.affiliation: "Petru Poni" Institute of Macromolecular Chemistry, 41-A Grigore Ghica Voda Alley, RO-700487 Iasi, Romania.
authors.forename: M
authors.initials: M
authors.lastname: Bercea
authors.affiliation: "Petru Poni" Institute of Macromolecular Chemistry, 41-A Grigore Ghica Voda Alley, RO-700487 Iasi, Romania.
authors.forename: M
authors.initials: M
authors.lastname: Asandulesa
authors.affiliation: Institut für Physikalische Chemie der Johannes Gutenberg Universität Mainz, Jakob Welder-Weg 11, D-55099 Mainz, Germany.
authors.forename: Bernhard A
authors.initials: BA
authors.lastname: Wolf
mesh_terms: D000818:Animals; D002417:Cattle; D006863:Hydrogen-Ion Concentration; D008958:Models, Molecular; D010455:Peptides / Q000737:chemistry*; D011485:Protein Binding; D011487:Protein Conformation; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D012996:Solutions; D014867:Water / Q000737:chemistry*
chemical_list: D010455:Peptides; D012996:Solutions; D014867:Water; C017645:polyaspartate; D012710:Serum Albumin, Bovine
keywords: Bovine serum albumin; Interpolymer complex; Poly(aspartic acid); Self-assembling