title: Effects of three different embryonic exposure modes of 2, 2', 4, 4'-tetrabromodiphenyl ether on the path angle and social activity of zebrafish larvae.
abstract: The toxicological research of polybrominated diphenyl ethers (PBDEs) has focused on its neurotoxicity; however, many questions still remain. For example, behavioral effects other than basic locomotion are seldom reported. To further evaluate the neurobehavioral toxicity of 2, 2', 4, 4'-tetrabromodiphenyl ether (BDE-47), a typical PBDE congener in animal tissues, we employed three different exposure modes, namely, continuous, early pulse, and interval exposure, to investigate the path angle and social activity changes of zebrafish larvae exposed to BDE-47 using automated equipment (Zebrabox). The results showed that different exposure modes might have different effects on the larval path angle and social activity. BDE-47 treatments caused more responsive turns in all exposure modes in the path angle test and more contacts in most of the two-fish social tests, indicating that the neurobehavior of larvae was disturbed by BDE-47. The light condition was also a key impact factor in the effects of BDE-47. The effects of BDE-47 were different during the dark and light conditions. Our study shows a useful neurobehavioral test method for environmental pollutant monitoring and further supports the utility of zebrafish to study neurobehavior, indicating that the path angle has the potential to be a practicable behavioral indicator.
authors.affiliation: State Key Laboratory of Pollution Control and Resources Reuse, College of Environmental Science and Engineering, Tongji University, Shanghai 200092, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Pollution Control and Resources Reuse, College of Environmental Science and Engineering, Tongji University, Shanghai 200092, China.
authors.forename: Xiaoling
authors.initials: X
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Pollution Control and Resources Reuse, College of Environmental Science and Engineering, Tongji University, Shanghai 200092, China.
authors.forename: Ruijie
authors.initials: R
authors.lastname: Pan
authors.affiliation: State Key Laboratory of Pollution Control and Resources Reuse, College of Environmental Science and Engineering, Tongji University, Shanghai 200092, China.
authors.forename: Ting
authors.initials: T
authors.lastname: Xu
authors.affiliation: Shanghai Collaborative Innovation Centre for WEEE Recycling, WEEE Research Centre of Shanghai Polytechnic University, Shanghai 201209, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Zhao
authors.affiliation: State Key Laboratory of Pollution Control and Resources Reuse, College of Environmental Science and Engineering, Tongji University, Shanghai 200092, China.
authors.forename: Wenping
authors.initials: W
authors.lastname: Huang
authors.affiliation: State Key Laboratory of Pollution Control and Resources Reuse, College of Environmental Science and Engineering, Tongji University, Shanghai 200092, China.
authors.forename: Youyu
authors.initials: Y
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Pollution Control and Resources Reuse, College of Environmental Science and Engineering, Tongji University, Shanghai 200092, China. Electronic address: yindq@tongji.edu.cn.
authors.forename: Daqiang
authors.initials: D
authors.lastname: Yin
mesh_terms: D000818:Animals; D001522:Behavior, Animal / Q000187:drug effects*; D004625:Embryo, Nonmammalian / Q000187:drug effects*; D004784:Environmental Monitoring / Q000379:methods*; D005260:Female; D055768:Halogenated Diphenyl Ethers / Q000633:toxicity*; D007814:Larva; D009043:Motor Activity / Q000187:drug effects; D012919:Social Behavior; D014874:Water Pollutants, Chemical / Q000633:toxicity*; D015027:Zebrafish / Q000196:embryology
chemical_list: D055768:Halogenated Diphenyl Ethers; D014874:Water Pollutants, Chemical; C511295:2,2',4,4'-tetrabromodiphenyl ether
keywords: BDE-47; Exposure mode; Neurobehavioral effect; Zebrafish larvae