title: Colwellia marinimaniae sp. nov., a hyperpiezophilic species isolated from an amphipod within the Challenger Deep, Mariana Trench.
abstract: An obligately piezophilic strain was isolated from an amphipod crustacean obtained in the Challenger Deep region of the Mariana Trench during the DEEPSEA CHALLENGE expedition. The strain, MTCD1T, grew at extremely high hydrostatic pressures, with a growth range of 80-140 MPa (optimum, 120 MPa) at 6 °C. Phylogenetic analyses based on the 16S rRNA gene sequence indicate that it is closely affiliated with the genus Colwellia. Comparative 16S rRNA gene sequence analyses revealed 95.7, 95.5 and 95.2 % similarity to Colwellia maris ABE-1T, Colwellia piezophila Y233GT and Colwellia psychrerythraea ATCC 27364T, respectively. The major cellular fatty acids were C16 : 1, C16 : 0 and C22 : 6 (docosahexaenoic acid), and the sole isoprenoid quinone produced was ubiqinone-8. DNA G+C content was 48.6 mol%. The strain was positive for oxidase and catalase activities. Based on the results from this study, strain MTCD1T is a novel Gram-negative species of the genus Colwellia, and the name Colwellia marinimaniae sp. nov. (type strain MTCD1T=ATCC TSD-5T=JCM 30270T) is proposed. It is the most piezophilic organism yet described.
authors.affiliation: Department of Material Science, National Institute of Technology, Wakayama College, 77 Noshima, Nada-cho, Gobo, Wakayama 644-0023, Japan.
authors.forename: Masataka
authors.initials: M
authors.lastname: Kusube
authors.affiliation: Marine Biology Research Division, Scripps Institution of Oceanography, University of California San Diego, La Jolla, CA 92093-0202, USA.
authors.forename: Than S
authors.initials: TS
authors.lastname: Kyaw
authors.affiliation: Department of Material Science, National Institute of Technology, Wakayama College, 77 Noshima, Nada-cho, Gobo, Wakayama 644-0023, Japan.
authors.forename: Kumiko
authors.initials: K
authors.lastname: Tanikawa
authors.affiliation: Marine Biology Research Division, Scripps Institution of Oceanography, University of California San Diego, La Jolla, CA 92093-0202, USA.
authors.forename: Roger A
authors.initials: RA
authors.lastname: Chastain
authors.affiliation: Global Ocean Dynamics. Global Ocean Design, 7955 Silverton Ave., Suite 1208, San Diego, CA 92126, USA.
authors.forename: Kevin M
authors.initials: KM
authors.lastname: Hardy
authors.affiliation: Avatar Alliance Foundation, 16255 Ventura Blvd. Suite 525, Encino, CA 91436, USA.
authors.forename: James
authors.initials: J
authors.lastname: Cameron
authors.affiliation: Marine Biology Research Division, Scripps Institution of Oceanography, University of California San Diego, La Jolla, CA 92093-0202, USA.
authors.forename: Douglas H
authors.initials: DH
authors.lastname: Bartlett
mesh_terms: D034241:Alteromonadaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D033304:Amphipoda / Q000382:microbiology*; D000818:Animals; D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D006874:Hydrostatic Pressure; D009693:Nucleic Acid Hybridization; D010140:Pacific Ocean; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D014451:Ubiquinone / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D014451:Ubiquinone; C030778:ubiquinone 8