title: Start-up and bacterial community compositions of partial nitrification in moving bed biofilm reactor.
abstract: Partial nitrification (PN) has been considered as one of the promising processes for pretreatment of ammonium-rich wastewater. In this study, a kind of novel carriers with enhanced hydrophilicity and electrophilicity was implemented in a moving bed biofilm reactor (MBBR) to start up PN process. Results indicated that biofilm formation rate was higher on modified carriers. In comparison with the reactor filled with traditional carriers (start-up period of 21 days), it took only 14 days to start up PN successfully with ammonia removal efficiency and nitrite accumulation rate of 90 and 91%, respectively, in the reactor filled with modified carriers. Evident changes of spatial distributions and community structures had been detected during the start-up. Free-floating cells existed in planktonic sludge, while these microorganisms trended to form flocs in the biofilm. High-throughput pyrosequencing results indicated that Nitrosomonas was the predominant ammonia-oxidizing bacterium (AOB) in the PN system, while Comamonas might also play a vital role for nitrogen oxidation. Additionally, some other bacteria such as Ferruginibacter, Ottowia, Saprospiraceae, and Rhizobacter were selected to establish stable footholds. This study would be potentially significant for better understanding the microbial features and developing efficient strategies accordingly for MBBR-based PN operation.
authors.affiliation: Key Laboratory of Industrial Ecology and Environmental Engineering (Ministry of Education), School of Environmental Science and Technology, Dalian University of Technology, Dalian, 116024, People's Republic of China. taoliu@dlut.edu.cn.
authors.forename: Tao
authors.initials: T
authors.lastname: Liu
authors.affiliation: Key Laboratory of Industrial Ecology and Environmental Engineering (Ministry of Education), School of Environmental Science and Technology, Dalian University of Technology, Dalian, 116024, People's Republic of China.
authors.forename: Yan-Jun
authors.initials: YJ
authors.lastname: Mao
authors.affiliation: Key Laboratory of Industrial Ecology and Environmental Engineering (Ministry of Education), School of Environmental Science and Technology, Dalian University of Technology, Dalian, 116024, People's Republic of China.
authors.forename: Yan-Ping
authors.initials: YP
authors.lastname: Shi
authors.affiliation: Key Laboratory of Industrial Ecology and Environmental Engineering (Ministry of Education), School of Environmental Science and Technology, Dalian University of Technology, Dalian, 116024, People's Republic of China.
authors.forename: Xie
authors.initials: X
authors.lastname: Quan
mesh_terms: D000641:Ammonia / Q000378:metabolism*; D001419:Bacteria / Q000145:classification / Q000235:genetics* / Q000378:metabolism; D018441:Biofilms / Q000254:growth & development*; D019149:Bioreactors; D020585:Comamonas / Q000254:growth & development / Q000378:metabolism; D059014:High-Throughput Nucleotide Sequencing; D006801:Humans; D059013:Microbial Consortia / Q000502:physiology; D058465:Nitrification; D009606:Nitrosomonas / Q000254:growth & development / Q000378:metabolism; D010084:Oxidation-Reduction; D010802:Phylogeny; D014865:Waste Disposal, Fluid; D062065:Wastewater / Q000382:microbiology*
chemical_list: D000641:Ammonia; D062065:Wastewater
keywords: Bacterial community; High-throughput pyrosequencing; Modified carriers; Moving bed biofilm reactor; Partial nitrification