title: Overexpression of a Pathogenesis-Related Protein 10 Enhances Biotic and Abiotic Stress Tolerance in Rice.
abstract: Pathogenesis-related proteins play multiple roles in plant development and biotic and abiotic stress tolerance. Here, we characterize a rice defense related gene named "jasmonic acid inducible pathogenesis-related class 10" (JIOsPR10) to gain an insight into its functional properties. Semi-quantitative RT-PCR analysis showed up-regulation ofJIOsPR10under salt and drought stress conditions. Constitutive over-expressionJIOsPR10in rice promoted shoot and root development in transgenic plants, however, their productivity was unaltered. Further experiments exhibited that the transgenic plants showed reduced susceptibility to rice blast fungus, and enhanced salt and drought stress tolerance as compared to the wild type. A comparative proteomic profiling of wild type and transgenic plants showed that overexpression ofJIOsPR10led to the differential modulation of several proteins mainly related with oxidative stresses, carbohydrate metabolism, and plant defense. Taken together, our findings suggest thatJIOsPR10plays important roles in biotic and abiotic stresses tolerance probably by activation of stress related proteins.
authors.affiliation: Department of Plant Microbe Interactions, Max Planck Institute for Plant Breeding Research, Cologne 50829, Germany; Division of Applied Life Science, Gyeongsang National University, Jinju 52828, Korea.
authors.forename: Jingni
authors.initials: J
authors.lastname: Wu
authors.affiliation: National Institute of Crop Science, Rural Development Administration, Suwon 16429, Korea.
authors.forename: Sang Gon
authors.initials: SG
authors.lastname: Kim
authors.affiliation: Division of Applied Life Science, Gyeongsang National University, Jinju 52828, Korea.
authors.forename: Kyu Young
authors.initials: KY
authors.lastname: Kang
authors.affiliation: College of Agriculture and Life Sciences, Seoul National University, Pyeongchang 25354, Korea.
authors.forename: Ju-Gon
authors.initials: JG
authors.lastname: Kim
authors.affiliation: National Institute of Agricultural Science, Rural Development Administration, Jeonju 54875, Korea.
authors.forename: Sang-Ryeol
authors.initials: SR
authors.lastname: Park
authors.affiliation: Department of Plant Bioscience, Life and Industry Convergence Research Institute, Pusan National University, Miryang 50463, Korea.
authors.forename: Ravi
authors.initials: R
authors.lastname: Gupta
authors.affiliation: College of Life and Resource Science, Dankook University, Cheonan 31116, Korea.
authors.forename: Yong Hwan
authors.initials: YH
authors.lastname: Kim
authors.affiliation: Department of Plant Microbe Interactions, Max Planck Institute for Plant Breeding Research, Cologne 50829, Germany.
authors.forename: Yiming
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Plant Bioscience, Life and Industry Convergence Research Institute, Pusan National University, Miryang 50463, Korea.
authors.forename: Sun Tae
authors.initials: ST
authors.lastname: Kim
keywords: JIOsPR10; Magnaporthe oryzae; abiotic stress; proteomics; rice