title: Antibacterial Potential ofJatropha curcasSynthesized Silver Nanoparticles against Food Borne Pathogens.
abstract: The aqueous leaf extract ofJatropha curcaswas used for the synthesis of silver nanoparticles (Jc-AgNps) which were further evaluated for its antibacterial potential against food borne pathogens.J. curcasleaf extract could synthesize stable silver nanoparticles (Zeta potential: -23.4 mV) with absorption band at 430 nm. Fourier transform infrared spectroscopy indicated various biological compounds responsible for capping and stabilizing Jc-AgNps in suspension, while the presence of silver was authenticated by scanning electron microscopy (SEM) equipped with energy-dispersive X-ray. Jc-AgNps were confirmed to be uniform in shape, size and behavior through dynamic light scattering, transmission electron microscopy (TEM), X-ray diffraction, SEM, and atomic force microscopy (AFM) analysis. To investigate the antibacterial activity, disk diffusion and microplate dilution assays were performed and zone of inhibition (ZOI) as well as minimum inhibitory/bactericidal concentrations (MIC/MBCs) were evaluated against selected bacterial strains. Overall results showed thatEscherichia coli(ZOI: 23 mm, MBC: 0.010 mg/ml) was the most sensitive organism, whereasStaphylococcus aureus(ZOI: 14.66 mm, MBC: 0.041 mg/ml) andSalmonella enterica(ZOI: 16.66 mm, MBC: 0.041 mg/ml) were the least sensitive against Jc-AgNps. The detailed microscopic investigations using SEM, TEM, and AFM were performed to understand the antibacterial impacts of Jc-AgNps againstListeria monocytogenes. SEM and TEM analysis showed the clear deformation and disintegration of treatedL. monocytogenescells, whereas AFM established a decrease in the height and cell surface roughness (root mean square value) in the treatedL. monocytogenes.
authors.affiliation: Applied Microbiology Laboratory, Centre for Rural Development and Technology, Indian Institute of Technology Delhi New Delhi, India.
authors.forename: Nitin
authors.initials: N
authors.lastname: Chauhan
authors.affiliation: Applied Microbiology Laboratory, Centre for Rural Development and Technology, Indian Institute of Technology DelhiNew Delhi, India; Cytokine Research Laboratory, Department of Experimental Therapeutics, The University of Texas MD Anderson Cancer CentreHouston, TX, USA.
authors.forename: Amit K
authors.initials: AK
authors.lastname: Tyagi
authors.affiliation: Applied Microbiology Laboratory, Centre for Rural Development and Technology, Indian Institute of Technology Delhi New Delhi, India.
authors.forename: Pushpendar
authors.initials: P
authors.lastname: Kumar
authors.affiliation: Applied Microbiology Laboratory, Centre for Rural Development and Technology, Indian Institute of Technology Delhi New Delhi, India.
authors.forename: Anushree
authors.initials: A
authors.lastname: Malik
keywords: Jatropha curcas; Listeria monocytogenes; antibacterial; silver nanoparticles