title: Effects of visfatin on the apoptosis of intestinal mucosal cells in immunological stressed rats.
abstract: This study was undertaken to determine if visfatin is involved in the inflammation or apoptosis introduced by LPS in rats. Forty 8-week old Wistar rats were divided into four groups (n=10 in each group) and injected with saline, visfatin, LPS and visfatin+LPS co-stimulated via caudal vein. The duodenum, jejunum and ileum were harvested from all the rats. Compared to the saline treated group, visfatin significantly increased the number of TUNEL-positive apoptotic cells and the expression of caspase-3 protein in intestinal mucosa. Similarly, ELISA and western blot analysis also showed the up-regulation of pro-caspase-3 and cleaved caspase-3 expression in the visfatin group compared to the control group. In contrast to LPS, visfatin down-regulated the expression of cleaved-caspase-3 in the visfatin+LPS co-stimulated group, resulting in a significant decrease in apoptosis in intestinal mucosal cells. We observed more pro-caspase-3 positive cells in the visfatin+LPS co-stimulated group. The results indicate that, in the presence of LPS, visfatin plays an important role in the regulation of cell apoptosis and inflammation.
authors.affiliation: College of Animal Science and Technology, Huazhong Agricultural University, Wuhan, Hubei 430070,China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: College of Animal Science and Technology, Huazhong Agricultural University, Wuhan, Hubei 430070,China.
authors.forename: Huai-Rui
authors.initials: HR
authors.lastname: Yuan
authors.affiliation: College of Animal Science and Technology, Huazhong Agricultural University, Wuhan, Hubei 430070,China.
authors.forename: Lu
authors.initials: L
authors.lastname: Cui
authors.affiliation: College of Animal Science and Technology, Huazhong Agricultural University, Wuhan, Hubei 430070,China.
authors.forename: Abdur Rahman
authors.initials: AR
authors.lastname: Ansari
authors.affiliation: College of Animal Science and Technology, Huazhong Agricultural University, Wuhan, Hubei 430070,China.
authors.forename: Ke
authors.initials: K
authors.lastname: Xiao
authors.affiliation: College of Animal Science and Technology, Huazhong Agricultural University, Wuhan, Hubei 430070,China.
authors.forename: You
authors.initials: Y
authors.lastname: Luo
authors.affiliation: College of Animal Science and Technology, Huazhong Agricultural University, Wuhan, Hubei 430070,China.
authors.forename: Xin-Tong
authors.initials: XT
authors.lastname: Wu
authors.affiliation: College of Animal Science and Technology, Huazhong Agricultural University, Wuhan, Hubei 430070,China.
authors.forename: Liang
authors.initials: L
authors.lastname: Guo
authors.affiliation: College of Animal Science and Technology, Huazhong Agricultural University, Wuhan, Hubei 430070,China.
authors.forename: Faheem Ahmed
authors.initials: FA
authors.lastname: Khan
authors.affiliation: College of Animal Science and Technology, Huazhong Agricultural University, Wuhan, Hubei 430070,China.
authors.forename: Zhi
authors.initials: Z
authors.lastname: Yang
authors.affiliation: College of Animal Science and Technology, Huazhong Agricultural University, Wuhan, Hubei 430070,China. Electronic address: songh2007@mail.hzau.edu.cn.
authors.forename: Hui
authors.initials: H
authors.lastname: Song
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D053148:Caspase 3 / Q000235:genetics / Q000378:metabolism; D004338:Drug Combinations; D004386:Duodenum / Q000166:cytology / Q000187:drug effects / Q000276:immunology; D004847:Epithelial Cells / Q000166:cytology / Q000187:drug effects* / Q000276:immunology; D015870:Gene Expression; D007082:Ileum / Q000166:cytology / Q000187:drug effects / Q000276:immunology; D018928:Immunity, Mucosal / Q000187:drug effects*; D020287:In Situ Nick-End Labeling; D007413:Intestinal Mucosa / Q000166:cytology / Q000187:drug effects* / Q000276:immunology; D007583:Jejunum / Q000166:cytology / Q000187:drug effects / Q000276:immunology; D008070:Lipopolysaccharides / Q000494:pharmacology*; D054409:Nicotinamide Phosphoribosyltransferase / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D004338:Drug Combinations; D008070:Lipopolysaccharides; D054409:Nicotinamide Phosphoribosyltransferase; C505410:Casp3 protein, rat; D053148:Caspase 3
keywords: Apoptosis; Caspase-3; Intestine mucosal; LPS induced; Visfatin