title: Bacteriological Profile of Isolates From Urine Samples in Patients of Benign Prostatic Hyperplasia and or Prostatitis Showing Lower Urinary Tract Symptoms.
abstract: BACKGROUND
  The incidence of Benign Prostatic Hyperplasia (BPH) or Prostatitis is increasing considerably worldwide. The Lower Urinary Tract Symptoms (LUTS) due to bacterial aetiology are one of the common factors for the complications among the patients.
  
  
  OBJECTIVE
  To determine the bacterial agents and their antibiotic sensitivity pattern from the urine samples of patients of BPH or Prostatitis showing symptoms of LUTS.
  
  
  METHODS
  The cross-sectional study was carried out in the Department of Microbiology of Rohilkhand Medical College and Hospital of Northern India from June 2014 to May 2015. A total of 105 urine specimens from patients of BPH and/ or Prostatitis were cultured by a semi-quantitative method. The isolated bacteria were identified by colony morphology, Gram's staining, motility and biochemical tests. Antibiotic sensitivity was done according to the CLSI 2007 guidelines by disc diffusion method. Data was analysed by SPSS and Microsoft office 2007. Proportions and percentages were used as statistical measures.
  
  
  RESULTS
  The urine cultures from patients with BPH and or chronic Prostatitis, showed n=66/105 (62.85%) culture positivity. Out of 66 isolates the frequency was in following orderEscherichia coli21/66 (31.81%),Klebsiellaspp 19/66 (28.78%),Staphylococcus aureus11/66 (16.66%),Pseudomonas aeruginosa(10.60%),Proteusspp,Enterococcusspp,Acinetobacterspp andCitrobacterspp. The most susceptible 1st, 2ndand 3rdline antibiotics for Gram negative isolates were ampicillin, amikacin and tigecycline respectively. Amongst the Gram positive isolates, the susceptible 1st, 2ndand 3rdline antibiotics were cefoxitin, vancomycin, teicoplanin and linezolid. Multidrug resistance was seen inEscherichia coli(n=6),Klebsiellaspp (n=7),Pseudomonas aeruginosa(n=4) andStaphylococcus aureus(n=3).
  
  
  CONCLUSIONS
  Based on the above findings we can say that accurate aetiology of the LUTS among the patients of BPH and/or Prostatitis is warranted to initiate the therapeutic management. Based on our study we state that the prime pathogens areE.coli,Klebsiellaamong Gram negative isolates andS. aureusamong Gram positive. The most sensitive drugs are aminoglycosides, tetracyclines and carbepenems for Gram neagtive isolates and oxazolidinones and glycopeptides among Gram positive isolates.
authors.affiliation: Assistant Professor, Department of Microbiology, RMCH , Bareilly, UP, India .
authors.forename: Prem Prakash
authors.initials: PP
authors.lastname: Mishra
authors.affiliation: Professor, Department of Microbiology, RMCH , Bareilly, UP, India .
authors.forename: Ved
authors.initials: V
authors.lastname: Prakash
authors.affiliation: Professor, Department of Surgery, RMCH , Bareilly, UP, India .
authors.forename: Kashmir
authors.initials: K
authors.lastname: Singh
authors.affiliation: PG Student, Department of Microbiology, RMCH , Bareilly, UP, India .
authors.forename: H
authors.initials: H
authors.lastname: Mog
authors.affiliation: PG Student, Department of Microbiology, RMCH , Bareilly, UP, India .
authors.forename: Sumit
authors.initials: S
authors.lastname: Agarwal
keywords: Bacteria; Prostate; Urological disorders