title: Comparison analysis of microRNAs in response to EV71 and CA16 infection in human bronchial epithelial cells by high-throughput sequencing to reveal differential infective mechanisms.
abstract: Hand, foot, and mouth disease (HFMD) mainly caused by Enterovirus 71 (EV71) and coxsackievirus A16 (CA16) infections which presented significantly different clinical manifestations. Nevertheless, the factors underlying these differences remain unclear. Recently, the functions of microRNAs (miRNAs) in pathogen-host interactions have been highlighted. Here, we performed comprehensive miRNA profiling in EV71- and CA16-infected human bronchial epithelial (16HBE) cells at multiple time points using high-throughput sequencing. The results showed that 154 known and 47 novel miRNAs exhibited remarkable differences in expression. Of these, 65 miRNAs, including 58 known and 7 novel miRNAs, presented opposite trends in EV71- and CA16-infected samples. Subsequently, we mainly focused on the 56 known differentially expressed miRNAs by further screening for targets prediction. GO and pathway analysis of these targets demonstrated that 18 biological processes, 7 molecular functions, 1 cellular component and 123 pathways were enriched. Among these pathways, Cadherin signalling pathway, Wnt signalling pathway and angiogenesis showed significant alterations. The regulatory networks of these miRNAs with predicted targets, GOs, pathways and transcription factors were determined, which suggested that miRNAs displayed intricate regulatory mechanisms during the infection phase. Consequently, we specifically analysed the hierarchical GO categories of the predicted targets involved in adhesion. The results indicated that the distinct changes induced by EV71 and CA16 infection may be partly linked to airway epithelial barrier function. Taken together, our data provide useful insights that help elucidate the different host-pathogen interactions following EV71 and CA16 infection and might offer novel therapeutic targets for these infections.
authors.affiliation: Institute of Medical Biology, Chinese Academy of Medical Science and Peking Union Medical College, Kunming, 650118, China.
authors.forename: Yajie
authors.initials: Y
authors.lastname: Hu
authors.affiliation: Institute of Medical Biology, Chinese Academy of Medical Science and Peking Union Medical College, Kunming, 650118, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Song
authors.affiliation: Institute of Medical Biology, Chinese Academy of Medical Science and Peking Union Medical College, Kunming, 650118, China.
authors.forename: Longding
authors.initials: L
authors.lastname: Liu
authors.affiliation: Institute of Medical Biology, Chinese Academy of Medical Science and Peking Union Medical College, Kunming, 650118, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Li
authors.affiliation: Institute of Medical Biology, Chinese Academy of Medical Science and Peking Union Medical College, Kunming, 650118, China.
authors.forename: Beibei
authors.initials: B
authors.lastname: Tang
authors.affiliation: Institute of Medical Biology, Chinese Academy of Medical Science and Peking Union Medical College, Kunming, 650118, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Institute of Medical Biology, Chinese Academy of Medical Science and Peking Union Medical College, Kunming, 650118, China.
authors.forename: Jingjing
authors.initials: J
authors.lastname: Wang
authors.affiliation: Institute of Medical Biology, Chinese Academy of Medical Science and Peking Union Medical College, Kunming, 650118, China.
authors.forename: Lichun
authors.initials: L
authors.lastname: Wang
authors.affiliation: Institute of Medical Biology, Chinese Academy of Medical Science and Peking Union Medical College, Kunming, 650118, China.
authors.forename: Shengtao
authors.initials: S
authors.lastname: Fan
authors.affiliation: Institute of Medical Biology, Chinese Academy of Medical Science and Peking Union Medical College, Kunming, 650118, China.
authors.forename: Ming
authors.initials: M
authors.lastname: Feng
authors.affiliation: Institute of Medical Biology, Chinese Academy of Medical Science and Peking Union Medical College, Kunming, 650118, China. Electronic address: liqihan@imbcams.com.cn.
authors.forename: Qihan
authors.initials: Q
authors.lastname: Li
mesh_terms: D002460:Cell Line; D019295:Computational Biology / Q000379:methods; D029821:Enterovirus A, Human / Q000502:physiology*; D004769:Enterovirus Infections / Q000235:genetics* / Q000821:virology*; D004847:Epithelial Cells; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D063990:Gene Ontology; D053263:Gene Regulatory Networks; D059014:High-Throughput Nucleotide Sequencing; D054884:Host-Pathogen Interactions / Q000235:genetics*; D006801:Humans; D035683:MicroRNAs; D058977:Molecular Sequence Annotation; D009690:Nucleic Acid Conformation; D034622:RNA Interference; D020545:Respiratory Mucosa / Q000378:metabolism* / Q000821:virology*
chemical_list: D035683:MicroRNAs
keywords: Coxsackievirus A16 (CA16); Enterovirus 71 (EV71); High-throughput sequencing; Human bronchial epithelial (16HBE) cells; MicroRNAs (miRNAs)