title: Influence of ozonolysis time during sugarcane pretreatment: Effects on the fiber and enzymatic saccharification.
abstract: Modifications in sugarcane bagasse (SCB) from ozonolysis (O) NaOH (B) and ultrasound (U) (OBU) treatment for cellulosic ethanol production by enzymatic hydrolysis, were evaluated when increasing the exposure time of SCB to ozone. The lignin, cellulose, and hemicellulose after treatment were quantified: lignin removal and a consequent increase in cellulose content were shown using an infrared spectroscopic technique (ATR-FTIR) and chemical characterization. X-ray diffraction analysis (XRD) proved that OBU treatment does not affect the crystalline cellulose portion and electron microscopy techniques established that the fiber region most affected by the OBU treatment was the secondary cell wall, where the greatest lignin content is located. For OBU-60 treatment the lignin content was reduced and consequently there was a significant increase in cellulose content. After enzymatic hydrolysis, this pretreated SCB released 418mgglucose/g, corresponding to six times more than untreated SCB and a yield of 93% of the cellulose available.
authors.affiliation: Univ. Estadual Paulista - IBILCE/UNESP, São José do Rio Preto, São Paulo, Brazil. Electronic address: olavo_perrone@hotmail.com.
authors.forename: Olavo Micali
authors.initials: OM
authors.lastname: Perrone
authors.affiliation: Univ. Estadual Paulista - IBILCE/UNESP, São José do Rio Preto, São Paulo, Brazil.
authors.forename: Jessika Souza
authors.initials: JS
authors.lastname: Rossi
authors.affiliation: Univ. Estadual Paulista - IBILCE/UNESP, São José do Rio Preto, São Paulo, Brazil.
authors.forename: Marcia Maria de Souza
authors.initials: MMS
authors.lastname: Moretti
authors.affiliation: Univ. Estadual Paulista - IBILCE/UNESP, São José do Rio Preto, São Paulo, Brazil.
authors.forename: Christiane da Costa Carreira
authors.initials: CDCC
authors.lastname: Nunes
authors.affiliation: Univ. Estadual Paulista - IBILCE/UNESP, São José do Rio Preto, São Paulo, Brazil.
authors.forename: Sidnei Emilio
authors.initials: SE
authors.lastname: Bordignon
authors.affiliation: Univ. Estadual Paulista - IBILCE/UNESP, São José do Rio Preto, São Paulo, Brazil.
authors.forename: Eleni
authors.initials: E
authors.lastname: Gomes
authors.affiliation: Univ. Estadual Paulista - IBILCE/UNESP, São José do Rio Preto, São Paulo, Brazil.
authors.forename: Roberto
authors.initials: R
authors.lastname: Da-Silva
authors.affiliation: Univ. Estadual Paulista - IBILCE/UNESP, São José do Rio Preto, São Paulo, Brazil.
authors.forename: Mauricio
authors.initials: M
authors.lastname: Boscolo
mesh_terms: D001709:Biotechnology / Q000379:methods*; D002473:Cell Wall / Q000737:chemistry; D002480:Cellulase / Q000737:chemistry; D002482:Cellulose / Q000737:chemistry; D006868:Hydrolysis; D008031:Lignin / Q000737:chemistry; D008855:Microscopy, Electron, Scanning; D046529:Microscopy, Electron, Transmission; D010126:Ozone / Q000737:chemistry*; D011134:Polysaccharides / Q000737:chemistry; D031786:Saccharum / Q000737:chemistry* / Q000166:cytology; D017550:Spectroscopy, Fourier Transform Infrared; D014961:X-Ray Diffraction
chemical_list: D011134:Polysaccharides; D010126:Ozone; C007916:hemicellulose; D002482:Cellulose; D008031:Lignin; C027433:bagasse; D002480:Cellulase
keywords: Cellulosic ethanol; Enzymatic hydrolysis; Ozonolysis; Pretreatment; Sugarcane bagasse