title: Modulation of intestinal barrier function to ameliorate Salmonella infection in mice by oral administration of fermented milks produced with Lactobacillus plantarum MTCC 5690 - a probiotic strain of Indian gut origin.
abstract: Probiotic Lactobacillus plantarum MTCC 5690, a probiotic strain of Indian gut origin, and milk formulations produced with the same were explored in this study as biotherapeutics by evaluating their functional efficacy against Salmonella infection in mice. The efficacy of milk formulations (fermented/unfermented) of MTCC 5690 for enhancement of intestinal barrier function was determined by monitoring the permeability and histopathology of the intestine. Infected mice fed with probiotic Dahi, fermented probiotic drink and sweetened fermented probiotic drink maintained the health and integrity of the intestinal epithelium as compared to those fed with PBS, milk, unfermented probiotic milk and Dahi. Our relative expression data revealed that the changes caused by MTCC 5690 in intestinal barrier function components were established through modulation of the key regulatory receptors Toll-like receptor 2 and Toll-like receptor 4. The results suggest that fermented milks of MTCC 5690 could enhance the defences of the intestinal barrier in enteric infection condition and, therefore, can be explored as a dietary-based strategy to reduce Salmonella infection in the human gut.
authors.affiliation: Molecular Biology Unit, Indian Council of Agricultural Research (ICAR) - National Dairy Research Institute (NDRI), Karnal, Haryana 132001, India.
authors.forename: Namita
authors.initials: N
authors.lastname: Rokana
authors.affiliation: Molecular Biology Unit, Indian Council of Agricultural Research (ICAR) - National Dairy Research Institute (NDRI), Karnal, Haryana 132001, India.
authors.forename: Rajbir
authors.initials: R
authors.lastname: Singh
authors.affiliation: Molecular Biology Unit, Indian Council of Agricultural Research (ICAR) - National Dairy Research Institute (NDRI), Karnal, Haryana 132001, India.
authors.forename: Rashmi Hogarehalli
authors.initials: RH
authors.lastname: Mallappa
authors.affiliation: Molecular Biology Unit, Indian Council of Agricultural Research (ICAR) - National Dairy Research Institute (NDRI), Karnal, Haryana 132001, India.
authors.forename: Virender Kumar
authors.initials: VK
authors.lastname: Batish
authors.affiliation: Molecular Biology Unit, Indian Council of Agricultural Research (ICAR) - National Dairy Research Institute (NDRI), Karnal, Haryana 132001, India.
authors.forename: Sunita
authors.initials: S
authors.lastname: Grover
mesh_terms: D000284:Administration, Oral; D000818:Animals; D018988:Bacterial Translocation; D043302:Cultured Milk Products / Q000382:microbiology*; D004195:Disease Models, Animal; D005243:Feces / Q000382:microbiology; D007194:India; D007413:Intestinal Mucosa / Q000382:microbiology* / Q000473:pathology / Q000502:physiology; D007422:Intestines / Q000382:microbiology / Q000502:physiology*; D043183:Irritable Bowel Syndrome / Q000235:genetics; D048191:Lactiplantibacillus plantarum / Q000254:growth & development / Q000302:isolation & purification / Q000502:physiology*; D051379:Mice; D008892:Milk / Q000382:microbiology; D055262:Mucin-2 / Q000235:genetics; D016133:Polymerase Chain Reaction; D019936:Probiotics / Q000627:therapeutic use*; D012481:Salmonella Infections, Animal / Q000382:microbiology / Q000628:therapy*; D012486:Salmonella typhimurium / Q000502:physiology*; D051195:Toll-Like Receptor 2 / Q000235:genetics; D051197:Toll-Like Receptor 4 / Q000235:genetics
chemical_list: C524626:Muc2 protein, mouse; D055262:Mucin-2; D051195:Toll-Like Receptor 2; D051197:Toll-Like Receptor 4