title: Forensic genetic study of 29 Y-STRs in Korean population.
abstract: In this study, we compared two recently released commercial Y-chromosomal short tandem repeat (Y-STR) kits: the PowerPlex Y23 System (PPY23) and Yfiler® Plus PCR amplification kit (YPlus). We performed validation studies, including sensitivity, tolerance to PCR inhibitors, and mixture analysis, and a population genetics study using 306 unrelated South Korean males. PPY23 and YPlus showed similar sensitivity, but PPY23 showed higher tolerance to humic acid than YPlus. Furthermore, the detection rate of unique minor alleles called from male/male mixtures was higher for PPY23 than for YPlus. Comparing the newly added loci, the mean values of gene diversity for PPY23 and YPlus were 0.6715 and 0.8158, respectively. The discrimination capacity in the 306 unrelated South Korean males for PPY23 was 0.9837, and that for YPlus was 0.9935. These results will inform the selection of suitable Y-STR kits based on the purpose of forensic DNA analysis.
authors.affiliation: Forensic DNA Division, National Forensic Service, Wonju 26460, South Korea. Electronic address: jjy7@korea.kr.
authors.forename: Ju Yeon
authors.initials: JY
authors.lastname: Jung
authors.affiliation: Forensic DNA Division, National Forensic Service, Wonju 26460, South Korea. Electronic address: zlxl79@korea.kr.
authors.forename: Ji-Hye
authors.initials: JH
authors.lastname: Park
authors.affiliation: Forensic DNA Division, National Forensic Service, Wonju 26460, South Korea. Electronic address: liluadios@gmail.com.
authors.forename: Yu-Li
authors.initials: YL
authors.lastname: Oh
authors.affiliation: Forensic DNA Division, National Forensic Service, Wonju 26460, South Korea. Electronic address: hansol0908@gmail.com.
authors.forename: Han-Sol
authors.initials: HS
authors.lastname: Kwon
authors.affiliation: Forensic DNA Division, National Forensic Service, Wonju 26460, South Korea. Electronic address: hcpark79@korea.kr.
authors.forename: Hyun-Chul
authors.initials: HC
authors.lastname: Park
authors.affiliation: Forensic DNA Division, National Forensic Service, Wonju 26460, South Korea. Electronic address: pkh5817@naver.com.
authors.forename: Kyung-Hwa
authors.initials: KH
authors.lastname: Park
authors.affiliation: Forensic DNA Division, National Forensic Service, Wonju 26460, South Korea. Electronic address: ehkim0524@gmail.com.
authors.forename: Eun Hye
authors.initials: EH
authors.lastname: Kim
authors.affiliation: Forensic DNA Division, National Forensic Service, Wonju 26460, South Korea. Electronic address: dongsubs@korea.kr.
authors.forename: Dong-Sub
authors.initials: DS
authors.lastname: Lee
authors.affiliation: Forensic DNA Division, National Forensic Service, Wonju 26460, South Korea. Electronic address: neobios@korea.kr.
authors.forename: Si-Keun
authors.initials: SK
authors.lastname: Lim
mesh_terms: D041322:Chromosomes, Human, Y / Q000235:genetics*; D053803:Forensic Genetics; D014644:Genetic Variation; D005828:Genetics, Population; D006239:Haplotypes; D006801:Humans; D018895:Microsatellite Repeats / Q000235:genetics*; D016133:Polymerase Chain Reaction; D056910:Republic of Korea
keywords: Population genetics study; PowerPlex Y23 System; Validation study; Yfiler® Plus PCR amplification kit