title: The LBD12-1 Transcription Factor Suppresses Apical Meristem Size by Repressing Argonaute 10 Expression.
abstract: The shoot apical meristem (SAM) consists of a population of multipotent cells that generates all aerial structures and regenerates itself. SAM maintenance and lateral organ development are regulated by several complex signaling pathways, in which the Argonaute gene-mediated pathway plays a key role. One Argonaute gene, AGO10, functions as a microRNA locker that attenuates miR165/166 activity and positively regulates shoot apical meristem development, but little is known about when and how AGO10 is regulated at the transcriptional level. In this work, we showed that transgenic rice plants overexpressing LBD12-1, an LBD family transcription factor, exhibited stunted growth, twisted leaves, abnormal anthers, and reduced SAM size. Further research revealed that LBD12-1 directly binds to the promoter region and represses the expression of AGO10. Overexpression of AGO10 in an LBD12-1 overexpression background rescued the growth defect phenotype of LBD12-1-overexpressing plants. The expression of LBD12-1 and its binding ability to the AGO10 promoter is induced by stress. lbd12-1 loss-of-function mutants showed similar phenotypes and SAM size to the wild type under normal conditions, but lbd12-1 had a larger SAM under salt stress. Our findings provide novel insights into the regulatory mechanism of AGO10 by which SAM size is controlled under stress conditions.
authors.affiliation: National Key Facility for Crop Resources and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China (W.M., F.W., P.S., X.W., Z.Z., Q.L., Z.C., J. Wang, S.Z., X.Z., X.G., H.W., C.W., H.Z., J. Wan); and.|State Key Laboratory for Crop Genetics and Germplasm Enhancement, Jiangsu Plant Gene Engineering Research Center, Nanjing Agricultural University, Nanjing 210095, China (K.Z., H.Z., J.H., J. Wan).
authors.forename: Weiwei
authors.initials: W
authors.lastname: Ma
authors.affiliation: National Key Facility for Crop Resources and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China (W.M., F.W., P.S., X.W., Z.Z., Q.L., Z.C., J. Wang, S.Z., X.Z., X.G., H.W., C.W., H.Z., J. Wan); and.|State Key Laboratory for Crop Genetics and Germplasm Enhancement, Jiangsu Plant Gene Engineering Research Center, Nanjing Agricultural University, Nanjing 210095, China (K.Z., H.Z., J.H., J. Wan).
authors.forename: Fuqing
authors.identifier: 0000-0002-4172-3868
authors.initials: F
authors.lastname: Wu
authors.affiliation: National Key Facility for Crop Resources and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China (W.M., F.W., P.S., X.W., Z.Z., Q.L., Z.C., J. Wang, S.Z., X.Z., X.G., H.W., C.W., H.Z., J. Wan); and.|State Key Laboratory for Crop Genetics and Germplasm Enhancement, Jiangsu Plant Gene Engineering Research Center, Nanjing Agricultural University, Nanjing 210095, China (K.Z., H.Z., J.H., J. Wan).
authors.forename: Peike
authors.initials: P
authors.lastname: Sheng
authors.affiliation: National Key Facility for Crop Resources and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China (W.M., F.W., P.S., X.W., Z.Z., Q.L., Z.C., J. Wang, S.Z., X.Z., X.G., H.W., C.W., H.Z., J. Wan); and.|State Key Laboratory for Crop Genetics and Germplasm Enhancement, Jiangsu Plant Gene Engineering Research Center, Nanjing Agricultural University, Nanjing 210095, China (K.Z., H.Z., J.H., J. Wan).
authors.forename: Xiaole
authors.initials: X
authors.lastname: Wang
authors.affiliation: National Key Facility for Crop Resources and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China (W.M., F.W., P.S., X.W., Z.Z., Q.L., Z.C., J. Wang, S.Z., X.Z., X.G., H.W., C.W., H.Z., J. Wan); and.|State Key Laboratory for Crop Genetics and Germplasm Enhancement, Jiangsu Plant Gene Engineering Research Center, Nanjing Agricultural University, Nanjing 210095, China (K.Z., H.Z., J.H., J. Wan).
authors.forename: Zhe
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: National Key Facility for Crop Resources and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China (W.M., F.W., P.S., X.W., Z.Z., Q.L., Z.C., J. Wang, S.Z., X.Z., X.G., H.W., C.W., H.Z., J. Wan); and.|State Key Laboratory for Crop Genetics and Germplasm Enhancement, Jiangsu Plant Gene Engineering Research Center, Nanjing Agricultural University, Nanjing 210095, China (K.Z., H.Z., J.H., J. Wan).
authors.forename: Kunneng
authors.initials: K
authors.lastname: Zhou
authors.affiliation: National Key Facility for Crop Resources and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China (W.M., F.W., P.S., X.W., Z.Z., Q.L., Z.C., J. Wang, S.Z., X.Z., X.G., H.W., C.W., H.Z., J. Wan); and.|State Key Laboratory for Crop Genetics and Germplasm Enhancement, Jiangsu Plant Gene Engineering Research Center, Nanjing Agricultural University, Nanjing 210095, China (K.Z., H.Z., J.H., J. Wan).
authors.forename: Huan
authors.identifier: 0000-0002-0257-0248
authors.initials: H
authors.lastname: Zhang
authors.affiliation: National Key Facility for Crop Resources and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China (W.M., F.W., P.S., X.W., Z.Z., Q.L., Z.C., J. Wang, S.Z., X.Z., X.G., H.W., C.W., H.Z., J. Wan); and.|State Key Laboratory for Crop Genetics and Germplasm Enhancement, Jiangsu Plant Gene Engineering Research Center, Nanjing Agricultural University, Nanjing 210095, China (K.Z., H.Z., J.H., J. Wan).
authors.forename: Jinlong
authors.initials: J
authors.lastname: Hu
authors.affiliation: National Key Facility for Crop Resources and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China (W.M., F.W., P.S., X.W., Z.Z., Q.L., Z.C., J. Wang, S.Z., X.Z., X.G., H.W., C.W., H.Z., J. Wan); and.|State Key Laboratory for Crop Genetics and Germplasm Enhancement, Jiangsu Plant Gene Engineering Research Center, Nanjing Agricultural University, Nanjing 210095, China (K.Z., H.Z., J.H., J. Wan).
authors.forename: Qibin
authors.initials: Q
authors.lastname: Lin
authors.affiliation: National Key Facility for Crop Resources and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China (W.M., F.W., P.S., X.W., Z.Z., Q.L., Z.C., J. Wang, S.Z., X.Z., X.G., H.W., C.W., H.Z., J. Wan); and.|State Key Laboratory for Crop Genetics and Germplasm Enhancement, Jiangsu Plant Gene Engineering Research Center, Nanjing Agricultural University, Nanjing 210095, China (K.Z., H.Z., J.H., J. Wan).
authors.forename: Zhijun
authors.identifier: 0000-0002-2768-8162
authors.initials: Z
authors.lastname: Cheng
authors.affiliation: National Key Facility for Crop Resources and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China (W.M., F.W., P.S., X.W., Z.Z., Q.L., Z.C., J. Wang, S.Z., X.Z., X.G., H.W., C.W., H.Z., J. Wan); and.|State Key Laboratory for Crop Genetics and Germplasm Enhancement, Jiangsu Plant Gene Engineering Research Center, Nanjing Agricultural University, Nanjing 210095, China (K.Z., H.Z., J.H., J. Wan).
authors.forename: Jiulin
authors.identifier: 0000-0003-4375-4892
authors.initials: J
authors.lastname: Wang
authors.affiliation: National Key Facility for Crop Resources and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China (W.M., F.W., P.S., X.W., Z.Z., Q.L., Z.C., J. Wang, S.Z., X.Z., X.G., H.W., C.W., H.Z., J. Wan); and.|State Key Laboratory for Crop Genetics and Germplasm Enhancement, Jiangsu Plant Gene Engineering Research Center, Nanjing Agricultural University, Nanjing 210095, China (K.Z., H.Z., J.H., J. Wan).
authors.forename: Shanshan
authors.initials: S
authors.lastname: Zhu
authors.affiliation: National Key Facility for Crop Resources and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China (W.M., F.W., P.S., X.W., Z.Z., Q.L., Z.C., J. Wang, S.Z., X.Z., X.G., H.W., C.W., H.Z., J. Wan); and.|State Key Laboratory for Crop Genetics and Germplasm Enhancement, Jiangsu Plant Gene Engineering Research Center, Nanjing Agricultural University, Nanjing 210095, China (K.Z., H.Z., J.H., J. Wan).
authors.forename: Xin
authors.identifier: 0000-0002-2519-4441
authors.initials: X
authors.lastname: Zhang
authors.affiliation: National Key Facility for Crop Resources and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China (W.M., F.W., P.S., X.W., Z.Z., Q.L., Z.C., J. Wang, S.Z., X.Z., X.G., H.W., C.W., H.Z., J. Wan); and.|State Key Laboratory for Crop Genetics and Germplasm Enhancement, Jiangsu Plant Gene Engineering Research Center, Nanjing Agricultural University, Nanjing 210095, China (K.Z., H.Z., J.H., J. Wan).
authors.forename: Xiuping
authors.identifier: 0000-0002-4936-7562
authors.initials: X
authors.lastname: Guo
authors.affiliation: National Key Facility for Crop Resources and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China (W.M., F.W., P.S., X.W., Z.Z., Q.L., Z.C., J. Wang, S.Z., X.Z., X.G., H.W., C.W., H.Z., J. Wan); and.|State Key Laboratory for Crop Genetics and Germplasm Enhancement, Jiangsu Plant Gene Engineering Research Center, Nanjing Agricultural University, Nanjing 210095, China (K.Z., H.Z., J.H., J. Wan).
authors.forename: Haiyang
authors.identifier: 0000-0002-1302-5747
authors.initials: H
authors.lastname: Wang
authors.affiliation: National Key Facility for Crop Resources and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China (W.M., F.W., P.S., X.W., Z.Z., Q.L., Z.C., J. Wang, S.Z., X.Z., X.G., H.W., C.W., H.Z., J. Wan); and.|State Key Laboratory for Crop Genetics and Germplasm Enhancement, Jiangsu Plant Gene Engineering Research Center, Nanjing Agricultural University, Nanjing 210095, China (K.Z., H.Z., J.H., J. Wan).
authors.forename: Chuanyin
authors.initials: C
authors.lastname: Wu
authors.affiliation: National Key Facility for Crop Resources and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China (W.M., F.W., P.S., X.W., Z.Z., Q.L., Z.C., J. Wang, S.Z., X.Z., X.G., H.W., C.W., H.Z., J. Wan); and.|State Key Laboratory for Crop Genetics and Germplasm Enhancement, Jiangsu Plant Gene Engineering Research Center, Nanjing Agricultural University, Nanjing 210095, China (K.Z., H.Z., J.H., J. Wan).
authors.forename: Huqu
authors.initials: H
authors.lastname: Zhai
authors.affiliation: National Key Facility for Crop Resources and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China (W.M., F.W., P.S., X.W., Z.Z., Q.L., Z.C., J. Wang, S.Z., X.Z., X.G., H.W., C.W., H.Z., J. Wan); and wanjianmin@caas.cn.|State Key Laboratory for Crop Genetics and Germplasm Enhancement, Jiangsu Plant Gene Engineering Research Center, Nanjing Agricultural University, Nanjing 210095, China (K.Z., H.Z., J.H., J. Wan) wanjianmin@caas.cn.
authors.forename: Jianmin
authors.identifier: 0000-0002-7813-4362
authors.initials: J
authors.lastname: Wan
mesh_terms: D017360:Arabidopsis / Q000235:genetics* / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D060565:Argonaute Proteins / Q000235:genetics* / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D018519:Meristem / Q000235:genetics / Q000254:growth & development*; D010802:Phylogeny; D030821:Plants, Genetically Modified; D011401:Promoter Regions, Genetic; D012097:Repressor Proteins / Q000235:genetics* / Q000378:metabolism; D013312:Stress, Physiological / Q000235:genetics; D014026:Nicotiana / Q000235:genetics; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism
chemical_list: D029681:Arabidopsis Proteins; D060565:Argonaute Proteins; D012097:Repressor Proteins; D014157:Transcription Factors; C529694:AT5G43810 protein, Arabidopsis