title: Novel Technologies for Optimal Strain Breeding.
abstract: The implementation of a knowledge-based bioeconomy requires the rapid development of highly efficient microbial production strains that are able to convert renewable carbon sources to value-added products, such as bulk and fine chemicals, pharmaceuticals, or proteins at industrial scale. Starting from classical strain breeding by random mutagenesis and screening in the 1950s via rational design by metabolic engineering initiated in the 1970s, a range of powerful new technologies have been developed in the past two decades that can revolutionize future strain engineering. In particular, next-generation sequencing technologies combined with new methods of genome engineering and high-throughput screening based on genetically encoded biosensors have allowed for new concepts. In this chapter, selected new technologies relevant for breeding microbial production strains with a special emphasis on amino acid producers will be summarized.
authors.affiliation: IBG-1: Biotechnology, Institute of Bio- and Geosciences, Forschungszentrum Jülich, 52425, Jülich, Germany. m.bott@fz-juelich.de.
authors.forename: Michael
authors.initials: M
authors.lastname: Bott
authors.affiliation: IBG-1: Biotechnology, Institute of Bio- and Geosciences, Forschungszentrum Jülich, 52425, Jülich, Germany.
authors.forename: Lothar
authors.initials: L
authors.lastname: Eggeling
mesh_terms: D001419:Bacteria / Q000145:classification; D018407:Bacterial Physiological Phenomena; D001426:Bacterial Proteins / Q000502:physiology*; D001688:Biological Products / Q000138:chemical synthesis / Q000378:metabolism*; D064112:Clustered Regularly Interspaced Short Palindromic Repeats / Q000235:genetics*; D003545:Cysteine / Q000502:physiology*; D005285:Fermentation / Q000502:physiology; D024861:Genetic Enhancement / Q000379:methods; D060847:Metabolic Engineering / Q000379:methods*
chemical_list: D001426:Bacterial Proteins; D001688:Biological Products; D003545:Cysteine
keywords: CRISPR-Cas9; Conjugation; FACS; Genetically encoded biosensors; Metabolism; Recombineering; Regulation; Transcriptional regulators