title: First case of sterility associated with sex chromosomal abnormalities in a jenny.
abstract: Chromosomal abnormalities are one of the main causes of genetic infertility in horses. Currently, their detection rate is rising due to the use of new diagnostic tools employing molecular markers linked to the sex chromosome pair. Despite genetic similarities, there are no previous reports of sterility associated with chromosomal abnormalities in the domestic donkey (Equus asinus). Hereby, we determined the presence of a chromosomal mosaicism in a female donkey with reproductive problems using molecular methodologies developed for horses. A two-and-a-half-year-old jenny characterized by morphological abnormalities of the reproductive tract was cytogenetically analysed using conventional and fluorescent techniques and a group of microsatellite markers (short tandem repeat, STR). At the same time, five ultrasound measures of the reproductive tract were taken and compared with eight contemporary jennies of the same breed. After slaughter, morphological examinations showed that the case study had a blind vaginal vestibule defining an empty pouch that covered the entrance of the cervical os. Histopathological studies demonstrated that this abnormal structure was compatible with a remnant hymen. Molecular markers, STR and fluorescent in situ hybridization determinations revealed that the animal was a 62, XX/61,X mosaic and, therefore, the first case of chromosomal abnormalities in the sex pair reported in donkeys.
authors.affiliation: Veterinary Reproduction Group AGR-275, Department of Animal Medicine and Surgery, Veterinary Teaching Hospital, University of Cordoba, Cordoba, Spain.
authors.forename: J
authors.initials: J
authors.lastname: Dorado
authors.affiliation: Laboratory of Animal Genomics, MERAGEM AGR-158 Research Group, Department of Genetics, University of Córdoba, Cordoba, Spain.
authors.forename: G
authors.initials: G
authors.lastname: Anaya
authors.affiliation: Department of Animal Genomics and Molecular Biology, National Research Institute of Animal Production, Balice, Poland.
authors.forename: M
authors.initials: M
authors.lastname: Bugno-Poniewierska
authors.affiliation: Laboratory of Animal Genomics, MERAGEM AGR-158 Research Group, Department of Genetics, University of Córdoba, Cordoba, Spain.
authors.forename: A
authors.initials: A
authors.lastname: Molina
authors.affiliation: Department of Anatomy and Comparative Pathology, University of Cordoba, Cordoba, Spain.
authors.forename: A
authors.initials: A
authors.lastname: Mendez-Sanchez
authors.affiliation: Veterinary Reproduction Group AGR-275, Department of Animal Medicine and Surgery, Veterinary Teaching Hospital, University of Cordoba, Cordoba, Spain.
authors.forename: I
authors.initials: I
authors.lastname: Ortiz
authors.affiliation: Laboratory of Applied and Molecular Animal Cytogenetics, MERAGEM AGR-158 Research Group, Department of Genetics, University of Cordoba, Cordoba, Spain.
authors.forename: M
authors.initials: M
authors.lastname: Moreno-Millán
authors.affiliation: Veterinary Reproduction Group AGR-275, Department of Animal Medicine and Surgery, Veterinary Teaching Hospital, University of Cordoba, Cordoba, Spain.
authors.forename: M
authors.initials: M
authors.lastname: Hidalgo
authors.affiliation: Facultad de Ciencias Veterinarias, IGEVET - Instituto de Genética Veterinaria UNLP - CONICET LA PLATA, Universidad Nacional de La Plata, La Plata, Argentina.
authors.forename: P
authors.initials: P
authors.lastname: Peral García
authors.affiliation: Facultad de Ciencias Veterinarias, IGEVET - Instituto de Genética Veterinaria UNLP - CONICET LA PLATA, Universidad Nacional de La Plata, La Plata, Argentina.
authors.forename: S
authors.initials: S
authors.lastname: Demyda-Peyrás
mesh_terms: D000818:Animals; D018397:Equidae / Q000235:genetics*; D005260:Female; D007247:Infertility, Female / Q000235:genetics*; D018895:Microsatellite Repeats; D012729:Sex Chromosome Aberrations / Q000662:veterinary*
keywords: Equus asinus; ZOO-FISH; chromosomal abnormalities; diagnostic methods; sterility