title: Production and purification of amylolytic enzymes for saccharification of microalgal biomass.
abstract: The aim of this study was the production of amylolytic enzymes by solid state or submerged fermentations (SSF or SF, respectively), followed by purification using chemical process or microfiltration and immobilization of purified enzymes in a polyurethane support. The free and immobilized enzymes obtained were used to evaluate enzymatic hydrolysis of the polysaccharides of Spirulina. Microfiltration of the crude extracts resulted in an increase in their specific activity and thermal stability at 40°C and 50°C for 24h, as compared to extracts obtained by SSF and SF. Immobilization of polyurethane purified enzyme produced yields of 332% and 205% for the enzymes obtained by SF and SSF, respectively. Free or immobilized enzymes favor the generation of fermentable sugar, being the application of the purified and immobilized enzymes in the hydrolysis of microalgal polysaccharides considered a promising alternative towards development of the bioethanol production process from microalgal biomass.
authors.affiliation: University of Passo Fundo, Campus I, km 171, BR 285, P.O. Box 611, CEP 99001-970 Passo Fundo, RS, Brazil.
authors.forename: Éllen Francine
authors.initials: ÉF
authors.lastname: Rodrigues
authors.affiliation: Regional and Integrate University of Upper Uruguay and Missions, URI - Erechim, Av. Sete de Setembro 1621, Erechim, RS 99700-000, Brazil.
authors.forename: Aline Matuella Moreira
authors.initials: AMM
authors.lastname: Ficanha
authors.affiliation: Regional and Integrate University of Upper Uruguay and Missions, URI - Erechim, Av. Sete de Setembro 1621, Erechim, RS 99700-000, Brazil.
authors.forename: Rogério Marcos
authors.initials: RM
authors.lastname: Dallago
authors.affiliation: Federal University of Fronteira Sul - Campus de Erechim, ERS 135, km 72, n° 200, 99700-970 Erechim, RS, Brazil.
authors.forename: Helen
authors.initials: H
authors.lastname: Treichel
authors.affiliation: University of Passo Fundo, Campus I, km 171, BR 285, P.O. Box 611, CEP 99001-970 Passo Fundo, RS, Brazil.
authors.forename: Christian Oliveira
authors.initials: CO
authors.lastname: Reinehr
authors.affiliation: University of Passo Fundo, Campus I, km 171, BR 285, P.O. Box 611, CEP 99001-970 Passo Fundo, RS, Brazil.
authors.forename: Tainara Paula
authors.initials: TP
authors.lastname: Machado
authors.affiliation: University of Passo Fundo, Campus I, km 171, BR 285, P.O. Box 611, CEP 99001-970 Passo Fundo, RS, Brazil.
authors.forename: Greice Borges
authors.initials: GB
authors.lastname: Nunes
authors.affiliation: University of Passo Fundo, Campus I, km 171, BR 285, P.O. Box 611, CEP 99001-970 Passo Fundo, RS, Brazil. Electronic address: lmcolla2@gmail.com.
authors.forename: Luciane Maria
authors.initials: LM
authors.lastname: Colla
mesh_terms: D000681:Amylases / Q000302:isolation & purification* / Q000378:metabolism*; D001234:Aspergillus niger / Q000201:enzymology*; D018533:Biomass; D001709:Biotechnology / Q000379:methods; D004795:Enzyme Stability; D004800:Enzymes, Immobilized / Q000737:chemistry / Q000378:metabolism*; D005285:Fermentation; D005374:Filtration / Q000379:methods; D006868:Hydrolysis; D058086:Microalgae / Q000378:metabolism*; D011134:Polysaccharides / Q000737:chemistry / Q000378:metabolism; D011140:Polyurethanes / Q000737:chemistry; D053545:Spirulina / Q000378:metabolism
chemical_list: D004800:Enzymes, Immobilized; D011134:Polysaccharides; D011140:Polyurethanes; D000681:Amylases
keywords: Biofuels; Fungal amylases; Immobilization; Microfiltration