title: Isolation and Characterization of a Bacteriophage Preying an Antifungal Bacterium.
abstract: SeveralBacillusspecies were isolated from rice field soils, and 16S rRNA gene sequence analysis showed thatBacillus cereuswas the most abundant. A strain named BC1 showed antifungal activity againstRhizoctonia solani. Bacteriophages infecting strain BC1 were isolated from the same soil sample. The isolated phage PK16 had an icosahedral head of 100 ± 5 nm and tail of 200 ± 5 nm, indicating that it belonged to the familyMyoviridae. Analysis of the complete linear dsDNA genome revealed a 158,127-bp genome with G + C content of 39.9% comprising 235 open reading frames as well as 19 tRNA genes (including 1 pseudogene). Blastp analysis showed that the proteins encoded by the PK16 genome had the closest hits to proteins of seven different bacteriophages. A neighbor-joining phylogenetic tree based on the major capsid protein showed a robust clustering of phage PK16 with phage JBP901 and BCP8-2 isolated from Korean fermented food.
authors.affiliation: Department of Microbiology, Pukyong National University, Busan 48547, Korea.
authors.forename: Aryan
authors.initials: A
authors.lastname: Rahimi-Midani
authors.affiliation: Department of Microbiology, Pukyong National University, Busan 48547, Korea.
authors.forename: Kyoung-Ho
authors.initials: KH
authors.lastname: Kim
authors.affiliation: Department of Applied Biology, Dong-A University, Busan 49315, Korea.
authors.forename: Seon-Woo
authors.initials: SW
authors.lastname: Lee
authors.affiliation: Department of Clinical Laboratory Science, Dong-Eui Institute of Technology, Busan 47230, Korea.
authors.forename: Sang Bong
authors.initials: SB
authors.lastname: Jung
authors.affiliation: Department of Microbiology, Pukyong National University, Busan 48547, Korea.
authors.forename: Tae-Jin
authors.initials: TJ
authors.lastname: Choi
keywords: Bacillus; Myoviridae; antifungal; interaction; multifactorial