title: Human hair-follicle associated pluripotent (hHAP) stem cells differentiate to cardiac-muscle cells.
abstract: We have previously demonstrated that nestin-expressing hair follicle-associated-pluripotent (HAP) stem cells are located in the bulge area. HAP stem cells have been previously shown to differentiate to neurons, glial cells, keratinocytes, smooth-muscle cells, melanocytes and cardiac-muscle cells in vitro. Subsequently, we demonstrated that HAP stem cells could effect nerve and spinal cord regeneration in mouse models, differentiating to Schwann cells and neurons. In previous studies, we established an efficient protocol for the differentiation of cardiac-muscle cells from mouse HAP stem cells. In the present study, we isolated the upper part of human hair follicles containing human HAP (hHAP) stem cells. The upper parts of human hair follicles were suspended in DMEM containing 10% FBS where they differentiated to cardiac-muscle cells as well as neurons, glial cells, keratinocytes and smooth-muscle cells. This method is appropriate for future use with human hair follicles to produce hHAP stem cells in sufficient quantities for future heart, nerve and spinal cord regeneration in the clinic.
authors.affiliation: a Department of Dermatology , Kitasato University School of Medicine , Minami Ward , Sagamihara , Japan.
authors.forename: Natsuko
authors.initials: N
authors.lastname: Tohgi
authors.affiliation: a Department of Dermatology , Kitasato University School of Medicine , Minami Ward , Sagamihara , Japan.
authors.forename: Koya
authors.initials: K
authors.lastname: Obara
authors.affiliation: a Department of Dermatology , Kitasato University School of Medicine , Minami Ward , Sagamihara , Japan.
authors.forename: Masateru
authors.initials: M
authors.lastname: Yashiro
authors.affiliation: a Department of Dermatology , Kitasato University School of Medicine , Minami Ward , Sagamihara , Japan.
authors.forename: Yuko
authors.initials: Y
authors.lastname: Hamada
authors.affiliation: a Department of Dermatology , Kitasato University School of Medicine , Minami Ward , Sagamihara , Japan.
authors.forename: Nobuko
authors.initials: N
authors.lastname: Arakawa
authors.affiliation: a Department of Dermatology , Kitasato University School of Medicine , Minami Ward , Sagamihara , Japan.
authors.forename: Sumiyuki
authors.initials: S
authors.lastname: Mii
authors.affiliation: a Department of Dermatology , Kitasato University School of Medicine , Minami Ward , Sagamihara , Japan.
authors.forename: Ryoichi
authors.initials: R
authors.lastname: Aki
authors.affiliation: b AntiCancer, Inc. , San Diego , CA , USA.|c Department of Surgery , University of California San Diego , CA , USA.
authors.forename: Robert M
authors.initials: RM
authors.lastname: Hoffman
authors.affiliation: a Department of Dermatology , Kitasato University School of Medicine , Minami Ward , Sagamihara , Japan.
authors.forename: Yasuyuki
authors.initials: Y
authors.lastname: Amoh
mesh_terms: D000328:Adult; D015415:Biomarkers / Q000378:metabolism; D002454:Cell Differentiation; D003114:Colony-Forming Units Assay; D005260:Female; D005434:Flow Cytometry; D018859:Hair Follicle / Q000166:cytology*; D006801:Humans; D008297:Male; D008875:Middle Aged; D032383:Myocytes, Cardiac / Q000166:cytology* / Q000378:metabolism; D039904:Pluripotent Stem Cells / Q000166:cytology* / Q000378:metabolism
chemical_list: D015415:Biomarkers
keywords: Hair follicle; cardiac-muscle cell; differentiation; stem cell