title: siRNA-mediated suppression of collagen type iv alpha 2 (COL4A2) mRNA inhibits triple-negative breast cancer cell proliferation and migration.
abstract: Triple-negative breast cancer (TNBC) is more aggressive than other breast cancer subtypes. Collagen type IV alpha 2 (COL4A2), a major component of the basement membrane, dynamically influences a wide range of biological processes, including cancer pathogenesis and progression. This study evaluated the effects of COL4A2 siRNA delivered by lentiviral vector to TNBC cells. COL4A2 siRNA lenti-viral vector was constructed and transfected into MDA-MB-231 and MDA-MB-468 cells. The COL4A2 mRNA levels were quantified by RT-PCR. CCK8 assay was performed to evaluate cell proliferation and migration. Cell migration and invasion assays were carried out using Transwell. Cell apoptosis and cell cycle analyses were conducted using flow cytometric approach. We found that COL4A2 mRNA levels were significantly down-regulated in MDA-MB-231 and MDA-MB-468 cells after transfection with COL4A2 siRNA. Furthermore, cell migration and proliferation were significantly decreased and the cell cycle was arrested. Our results indicated that COL4A2 siRNA significantly suppresses the migration and proliferation of TNBC cells. Inhibition of COL4A2 may be a new target for the prevention and treatment of TNBC.
authors.affiliation: Department of Breast Surgery, The First Affiliated Hospital of Shenzhen University, Second People's Hospital of Shen Zhen, Shen Zhen, 518035, China.
authors.forename: He
authors.initials: H
authors.lastname: JingSong
authors.affiliation: Department of Pathology, The First Affiliated Hospital of Shenzhen University, Second People's Hospital of Shen Zhen, Shen Zhen 518035, China.
authors.forename: Guan
authors.initials: G
authors.lastname: Hong
authors.affiliation: Department of Laboratory Medicine and Pathology, Masonic Cancer Center, University of Minnesota, UMN Twin Cities, MN 55455, USA.
authors.forename: Jianbo
authors.initials: J
authors.lastname: Yang
authors.affiliation: Shenzhen Key Laboratory of Translational Medicine of Tumor, Department of Cell Biology and Genetics, School of Medicine, Shenzhen University, Shenzhen, Guangdong, 518060, China.
authors.forename: Zheng
authors.initials: Z
authors.lastname: Duo
authors.affiliation: Department of Pharmacology, Shenzhen Key Laboratory of Translational Medicine of Tumor and Cancer Research Centre, School of Medicine, Shenzhen University, Shenzhen, 518060 China.
authors.forename: Fu
authors.initials: F
authors.lastname: Li
authors.affiliation: Department of Breast Surgery, The First Affiliated Hospital of Shenzhen University, Second People's Hospital of Shen Zhen, Shen Zhen, 518035, China.
authors.forename: Chen
authors.initials: C
authors.lastname: WeiCai
authors.affiliation: Department of Breast Surgery, The First Affiliated Hospital of Shenzhen University, Second People's Hospital of Shen Zhen, Shen Zhen, 518035, China.
authors.forename: Luo
authors.initials: L
authors.lastname: XueYing
authors.affiliation: Department of Breast Surgery, The First Affiliated Hospital of Shenzhen University, Second People's Hospital of Shen Zhen, Shen Zhen, 518035, China.
authors.forename: Mao
authors.initials: M
authors.lastname: YouSheng
authors.affiliation: Department of Breast Surgery, The First Affiliated Hospital of Shenzhen University, Second People's Hospital of Shen Zhen, Shen Zhen, 518035, China.
authors.forename: OuYang
authors.initials: O
authors.lastname: YiWen
authors.affiliation: Department of Breast Surgery, The First Affiliated Hospital of Shenzhen University, Second People's Hospital of Shen Zhen, Shen Zhen, 518035, China.
authors.forename: Pan
authors.initials: P
authors.lastname: Yue
authors.affiliation: Clinical Medical Research Center, Shen Zhen People's Hospital, The Second Clinical Medical College of Jinan University, 518020, China.
authors.forename: Chang
authors.initials: C
authors.lastname: Zou
mesh_terms: D017209:Apoptosis; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D024141:Collagen Type IV / Q000235:genetics*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D020868:Gene Silencing; D006801:Humans; D034741:RNA, Small Interfering / Q000494:pharmacology*; D064726:Triple Negative Breast Neoplasms / Q000235:genetics*; D015854:Up-Regulation
chemical_list: C502625:COL4A2 protein, human; D024141:Collagen Type IV; D034741:RNA, Small Interfering
keywords: COL4A2; lentiviral vector; small interfering RNA (siRNA); triple-negative breast cancer (TNBC)