title: Associations of DNMT3B -149C>T and -2437T>A polymorphisms and lung cancer risk in Chinese population.
abstract: BACKGROUND
  DNMT3B polymorphisms are associated with the susceptibility of lung cancer. DNMT3B -2437T>A is a novel polymorphism, and its influence on the risk of lung cancer in Chinese was investigated in this study. In addition, effect of DNMT3B -149C>T polymorphism on lung cancer was also explored.
  
  
  METHODS
  Genotyping in subjects were performed by PCR-RFLP. Haplotype frequencies were estimated by estimating haplotype software. Adjusted odds ratios (ORs) with 95% confidence intervals (CIs) were calculated by unconditional logistic regression analysis.
  
  
  RESULTS
  Neither of the two polymorphisms was correlated with lung cancer (-149C>T: CT+TT vs CC: OR = 0.78, 95%CI, 0.57 to 1.05, P = 0.361; -2437T>A: AT+AA vs TT: OR = 0.99, 95%CI, 0.74 to 1.33, P = 0.168). In stratification analysis, T-allele carrier genotype of -149C>T polymorphism resulted in a reduced lung cancer risk at stage II, compared with CC (OR = 0.46, 95%CI, 0.28 to 0.77, P = 0.023). In haplotype analysis, when -149C/-2437T was used as reference, the other combined genotypes of the two polymorphisms had no significant effect on lung cancer risk (P > 0.05).
  
  
  CONCLUSIONS
  The two DNMT3B polymorphisms are not correlated with lung cancer risk among Chinese population nor the haplotype of them.
authors.affiliation: Department of Neurology, the Fourth Hospital of Harbin Medical University, 37 Yiyuan Street, Harbin, Heilongjiang, 150001, China. gmingao@sina.com.
authors.forename: Min
authors.initials: M
authors.lastname: Gao
authors.affiliation: Department of Radiology, the Fourth Hospital of Harbin Medical University, Harbin, Heilongjiang, China.
authors.forename: Daqiang
authors.initials: D
authors.lastname: He
authors.affiliation: Department of Cardiology, the Fourth Hospital of Harbin Medical University, Harbin, Heilongjiang, China.
authors.forename: Fanji
authors.initials: F
authors.lastname: Meng
authors.affiliation: Department of Geriatrics, the Fourth Hospital of Harbin Medical University, Harbin, Heilongjiang, China.
authors.forename: Jianing
authors.initials: J
authors.lastname: Li
authors.affiliation: Department of Geriatrics, the Fourth Hospital of Harbin Medical University, Harbin, Heilongjiang, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Shen
mesh_terms: D000368:Aged; D054458:Amplified Fragment Length Polymorphism Analysis; D044466:Asian People / Q000235:genetics*; D016022:Case-Control Studies; D004248:DNA (Cytosine-5-)-Methyltransferases / Q000235:genetics*; D005260:Female; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006239:Haplotypes; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D016017:Odds Ratio; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide; D012307:Risk Factors; D000097584:DNA Methyltransferase 3B
chemical_list: D004248:DNA (Cytosine-5-)-Methyltransferases; D000097584:DNA Methyltransferase 3B
keywords: DNMT3B; Lung cancer; Methylation; Polymorphism; −149C>T; −2437T>A