title: Latent pH-responsive ratiometric fluorescent cluster based on self-assembled photoactivated SNARF derivatives.
abstract: We have developed a self-assembled fluorescent cluster comprising a seminaphthorhodafluor (SNARF) derivative protected by a photoremovableo-nitrobenzyl group. Prior to UV irradiation, a colorless and nonfluorescent cluster was spontaneously assembled in aqueous solution. After UV irradiation, the self-assembled cluster remained intact and showed a large enhancement in pH-responsive fluorescence. The unique pH responsive fluorescent cluster could be used as a dual-emissive ratiometric fluorescent pH probe not only in the test tube but also in HeLa cell cultures.
authors.affiliation: Institute of Advanced Energy, Kyoto University , Uji , Kyoto , Japan.
authors.forename: Eiji
authors.initials: E
authors.lastname: Nakata
authors.affiliation: Department of Life System, Institute of Technology and Science, Graduate School, The University of Tokushima , Tokushima , Japan.
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Yukimachi
authors.affiliation: Department of Life System, Institute of Technology and Science, Graduate School, The University of Tokushima , Tokushima , Japan.
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Uto
authors.affiliation: Department of Life System, Institute of Technology and Science, Graduate School, The University of Tokushima , Tokushima , Japan.
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Hori
authors.affiliation: Institute of Advanced Energy, Kyoto University , Uji , Kyoto , Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Morii
keywords: 101 Self-assembly / Self-organized materials; 208 Sensors and actuators; 30 Bio-inspired and biomedical materials; Self-assembly; fluorescent material; pH indicator; photoactivation; ratiometry