title: Identification of caleosin and oleosin in oil bodies of pine pollen.
abstract: Unique proteins including steroleosin, caleosin, oleosin-L, and oleosin-G have been identified in seed oil bodies of pine (Pinus massoniana). In this study, mature pollen grains with wing-like bladders were collected from pine (Pinus elliottii). Ultrastructural studies showed that oil bodies were present in pollen grains, but not the attached bladders, and the presence of oil bodies was further confirmed by fluorescent staining with BODIPY 493/503. Stable oil bodies were successfully purified from pine pollen grains, and analyzed to be mainly composed of triacylglycerols. Putative oleosin and caleosin in pine pollen oil bodies were detected by immunoassaying with antibodies against sesame seed caleosin and lily pollen oleosin. Complete cDNA fragments encoding these two pollen oil-body proteins were obtained by PCR cloning. Sequence alignment showed that pine pollen caleosin (27 kDa) was highly homologous to pine seed caleosin (28 kDa) except for the lack of an appendix of eight residues at the C-terminus in accord with the 1 kDa difference in their molecular masses. Pine pollen oleosin (15 kDa) was highly homologous to pine seed oleosin-G (14 kDa) except for an insertion of eight residues at the N-terminus in accord with the 1 kDa difference in their molecular masses.
authors.affiliation: Graduate Institute of Biotechnology, National Chung-Hsing University, Taichung 402, Taiwan.
authors.forename: Buntora
authors.initials: B
authors.lastname: Pasaribu
authors.affiliation: Taiwan Coral Research Center, National Museum of Marine Biology and Aquarium, Pingtung 944, Taiwan; Institute of Marine Biotechnology, National Dong-Hwa University, Pingtung 944, Taiwan.
authors.forename: Chii-Shiarng
authors.initials: CS
authors.lastname: Chen
authors.affiliation: Department of Forestry and Natural Resources, National Chiayi University, Chiayi 600, Taiwan.
authors.forename: Yue Ken
authors.initials: YK
authors.lastname: Liao
authors.affiliation: Taiwan Coral Research Center, National Museum of Marine Biology and Aquarium, Pingtung 944, Taiwan; Institute of Marine Biotechnology, National Dong-Hwa University, Pingtung 944, Taiwan. Electronic address: villy@nmmba.gov.tw.
authors.forename: Pei-Luen
authors.initials: PL
authors.lastname: Jiang
authors.affiliation: Graduate Institute of Biotechnology, National Chung-Hsing University, Taichung 402, Taiwan. Electronic address: TCTZEN@dragon.nchu.edu.tw.
authors.forename: Jason T C
authors.initials: JTC
authors.lastname: Tzen
mesh_terms: D015153:Blotting, Western; D002135:Calcium-Binding Proteins / Q000378:metabolism*; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel; D066292:Lipid Droplets / Q000378:metabolism*; D008055:Lipids / Q000737:chemistry; D010802:Phylogeny; D010940:Plant Proteins / Q000378:metabolism*; D011058:Pollen / Q000166:cytology / Q000378:metabolism* / Q000648:ultrastructure; D016415:Sequence Alignment
chemical_list: D002135:Calcium-Binding Proteins; D018076:DNA, Complementary; D008055:Lipids; D010940:Plant Proteins; C402836:caleosin protein, Sesamum indicum; C079355:oleosin protein, Pinus longaeva
keywords: Caleosin; Oil body; Oleosin; Pine; Pollen