title: Frequency of VanA, VanB and VanH variants amongst vancomycin-resistant enterococci isolated from patients in central region of Iran.
abstract: OBJECTIVE
  The aim of this study was to investigate the VRE frequency and the rate of each gene in isolated enterococci from patients with intestinal infection in the central region of Iran.
  
  
  BACKGROUND
  Enterococciinfections are a public health growing concern due to the glycopeptide antibiotics resistance especially vancomycin. Genes,vanA,B, andHcontribute to the influence of vancomycin-resistant enterococci (VRE).
  
  
  METHODS
  This study was conducted from January to July 2014 in Shahrood university hospital. Enterococci isolation and its antibacterial susceptibility were performed by culturing in Aesculin Azide agar and Kirby-Bauer method, respectively. Vancomycin-resistant genes were screened through conventional PCR, and subsequently sequenced.
  
  
  RESULTS
  Among 265 specimens, 100 isolates revealed enterococci, in whichE. faecalis(91%) andE. faecium(9%). The isolated enterococci were resistant to vancomycin (6%) and chloramphenicol (21%), whereas their large proportions (94% to 100%) were multi-drug resistant. All VRE isolates belonged toE. faecalis,conversely, theE. faeciumwere susceptible to the same antibiotic. BothvanAandvanHgenes were identified in all VRE isolates, although, novanBgene was indicated. Homology analysis of sequenced amplicons verified the full length compatibility to the worldwide reported genes.
  
  
  CONCLUSIONS
  The present study revealed VRE.faecalisin gastroenteritis patients and resistance factor forvanAandvanHgenes are coordinated. Since enterococci isolates were all multidrug resistance, increase in VRE.faecalisvanA / vanH in this area could be expected.
authors.affiliation: Islamic Azad University, Damghn Branch, Damghan, Iran.
authors.forename: Jaafar
authors.initials: J
authors.lastname: Rezvani
authors.affiliation: Semnan Biotechnology Research Center, Semnan University of Medical Sciences, Semnan, Iran.
authors.forename: Reza
authors.initials: R
authors.lastname: Nasr
authors.affiliation: Student Research Committee, Semnan University of Medical Sciences, Semnan, Iran.
authors.forename: Fatemeh
authors.initials: F
authors.lastname: T Shamsabadi
authors.affiliation: Semnan Biotechnology Research Center, Semnan University of Medical Sciences, Semnan, Iran.
authors.forename: Mohammad Reza
authors.initials: MR
authors.lastname: Akbari Eidgahi
keywords: E. faecalis; E. faecium; Patients; Vancomycin-resistance enterococci (VRE); vanA; vanB; vanH