title: Lip b 1 is a novel allergenic protein isolated from the booklouse, Liposcelis bostrychophila.
abstract: BACKGROUND
  Booklice, belonging to the order Psocoptera, are small household insect pests that are distributed worldwide. Liposcelis bostrychophila, a common home-inhabiting species of booklouse, infests old books, sheets of paper, and stored food. Recent entomological and serological studies demonstrated that L. bostrychophila accounted for the majority of detectable insects in house dust and could be a potent inducer of respiratory allergy. Our recent proteomic analysis identified a potent allergenic protein from L. bostrychophila, designated Lip b 1, and determined its partial amino acid sequences.
  
  
  METHODS
  Cloning of cDNAs for Lip b 1 was performed by large-scale transcriptome analysis (RNA-seq) and subsequent reverse transcription polymerase chain reaction. The full-length amino acid sequences deduced from Lip b 1 cDNAs were bioinformatically analyzed. The recombinant proteins of glutathione S-transferase (GST)-fused Lip b 1 were analyzed by Western blot and enzyme-linked immunosorbent assay.
  
  
  RESULTS
  Lip b 1 cDNAs encoding two types of 254-amino acid proteins were cloned. The clones shared 87% identity, and the deduced molecular weights and isoelectric points were consistent with those determined in our previous study. The two types of Lip b 1 proteins in the GST-fused form were similarly reactive with sera from allergic patients sensitized with L. bostrychophila.
  
  
  CONCLUSIONS
  Lip b 1 is a novel protein possibly causing booklouse allergy.
authors.affiliation: Department of Applied Life Sciences, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, Sakai, Japan.
authors.forename: O
authors.initials: O
authors.lastname: Ishibashi
authors.affiliation: Department of Applied Life Sciences, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, Sakai, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Sakuragi
authors.affiliation: Clinical Research Center for Allergy and Rheumatology, Sagamihara National Hospital, Sagamihara, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Fukutomi
authors.affiliation: Laboratory of Environmental Science, FCG Research Institute Incorporation, Tokyo, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Kawakami
authors.affiliation: Department of Veterinary Medicine, Iwate University, Morioka, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Kamata
authors.affiliation: Department of Applied Life Sciences, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, Sakai, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Sakurai
authors.affiliation: Department of Applied Life Sciences, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, Sakai, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Nakayama
authors.affiliation: NODAI Genome Research Center, Tokyo University of Agriculture, Tokyo, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Uchiyama
authors.affiliation: NODAI Genome Research Center, Tokyo University of Agriculture, Tokyo, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Kobayashi
authors.affiliation: Department of Agriculture, Tokyo University of Agriculture, Atsugi, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Kojima
authors.affiliation: Department of Applied Life Sciences, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, Sakai, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Inui
mesh_terms: D000485:Allergens / Q000235:genetics / Q000302:isolation & purification*; D000595:Amino Acid Sequence; D000818:Animals; D003001:Cloning, Molecular; D018076:DNA, Complementary; D006801:Humans; D006967:Hypersensitivity / Q000209:etiology; D019476:Insect Proteins / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D008003:Phthiraptera / Q000737:chemistry / Q000276:immunology*
chemical_list: D000485:Allergens; D018076:DNA, Complementary; D019476:Insect Proteins
keywords: 
  Liposcelis bostrychophila
  ; booklouse