title: Distinct roles of ASIC3 and TRPV1 receptors in electroacupuncture-induced segmental and systemic analgesia.
abstract: Previous studies have demonstrated the effects of different afferent fibers on electroacupuncture (EA)-induced analgesia. However, contributions of functional receptors expressed on afferent fibers to the EA analgesia remain unclear. This study investigates the roles of acid-sensing ion channel 3 (ASIC3) and transient receptor potential vanilloid 1 (TRPV1) receptors in EA-induced segmental and systemic analgesia. Effects of EA at acupoint ST36 with different intensities on the C-fiber reflex and mechanical and thermal pain thresholds were measured among the ASIC3-/-, TRPV1-/-, and C57BL/6 mice. Compared with C57BL/6 mice, the ipsilateral inhibition of EA with 0.8 C-fiber threshold (0.8Tc) intensity on C-fiber reflex was markedly reduced in ASIC3-/-mice, whereas the bilateral inhibition of 1.0 and 2.0Tc EA was significantly decreased in TRPV1-/-mice. The segmental increase in pain thresholds induced by 0.3 mA EA was significantly reduced in ASIC3-/-mice, whereas the systemic enhancement of 1.0 mA EA was markedly decreased in TRPV1-/-mice. Thus, segmental analgesia of EA with lower intensity is partially mediated by ASIC3 receptor on Aβ-fiber, whereas systemic analgesia induced by EA with higher intensity is more likely induced by TRPV1 receptor on Aδ- and C-fibers.
authors.affiliation: Institute of Acupuncture and Moxibustion, China Academy of Chinese Medical Sciences, Beijing, 100700, China.
authors.forename: Juanjuan
authors.initials: J
authors.lastname: Xin
authors.affiliation: Institute of Acupuncture and Moxibustion, China Academy of Chinese Medical Sciences, Beijing, 100700, China.
authors.forename: Yangshuai
authors.initials: Y
authors.lastname: Su
authors.affiliation: Institute of Acupuncture and Moxibustion, China Academy of Chinese Medical Sciences, Beijing, 100700, China.
authors.forename: Zhaokun
authors.initials: Z
authors.lastname: Yang
authors.affiliation: Institute of Acupuncture and Moxibustion, China Academy of Chinese Medical Sciences, Beijing, 100700, China.
authors.forename: Wei
authors.initials: W
authors.lastname: He
authors.affiliation: Institute of Acupuncture and Moxibustion, China Academy of Chinese Medical Sciences, Beijing, 100700, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Shi
authors.affiliation: Institute of Acupuncture and Moxibustion, China Academy of Chinese Medical Sciences, Beijing, 100700, China.
authors.forename: Xiaoyu
authors.initials: X
authors.lastname: Wang
authors.affiliation: Institute of Acupuncture and Moxibustion, China Academy of Chinese Medical Sciences, Beijing, 100700, China.
authors.forename: Ling
authors.initials: L
authors.lastname: Hu
authors.affiliation: Institute of Acupuncture and Moxibustion, China Academy of Chinese Medical Sciences, Beijing, 100700, China.
authors.forename: Xiaochun
authors.initials: X
authors.lastname: Yu
authors.affiliation: Institute of Acupuncture and Moxibustion, China Academy of Chinese Medical Sciences, Beijing, 100700, China. jxhtjb@263.net.
authors.forename: Xianghong
authors.initials: X
authors.lastname: Jing
authors.affiliation: Institute of Acupuncture and Moxibustion, China Academy of Chinese Medical Sciences, Beijing, 100700, China. zhubing@mail.cintcm.ac.cn.
authors.forename: Bing
authors.initials: B
authors.lastname: Zhu
mesh_terms: D062565:Acid Sensing Ion Channels / Q000235:genetics*; D015669:Acupuncture Points; D000698:Analgesia / Q000379:methods*; D000818:Animals; D015671:Electroacupuncture / Q000379:methods*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D036421:Nerve Fibers, Unmyelinated / Q000187:drug effects*; D059408:Pain Management; D050916:TRPV Cation Channels / Q000235:genetics*
chemical_list: C508378:ASIC3 protein, mouse; D062565:Acid Sensing Ion Channels; D050916:TRPV Cation Channels; C493921:TRPV1 protein, mouse
keywords: ASIC3; C-fiber reflex; TRPV1; analgesia; electroacupuncture