title: All-in-one dual-aptasensor capable of rapidly quantifying carcinoembryonic antigen.
abstract: Using a dual DNA aptamer (CEA aptamer linked to hemin aptamer), capable of rapidly capturing carcinoembryonic antigen (CEA) and hemin, an all-in-one dual-aptasensor with 1,1'-oxalyldiimidazole (ODI) chemiluminescence detection was developed for the early diagnosis of human cancer. CEA and hemin competitively bound with the dual DNA aptamer while the mixture in a detection cell was incubated for 30min at room temperature. When Amplex Red and H2O2were added in the detection cell after the incubation, the yield of resorufin formed from the reaction Amplex Red and H2O2depended on the concentration of HRP-mimicking G-quardruplex DNAzyme formed from the binding interaction between hemin and the dual DNA aptamer. Bright red light was observed with the addition of ODI and H2O2in the detection cell containing resorufin. Relative CL intensity of all-in-one dual-aptasensor, operated with the competitive reaction of CEA and hemin in the presence of the dual aptamer, was exponentially decreased with the increase of CEA concentration in human serum. The limit of detection (LOD=3σ) of the all-in-one dual-aptasensor which operated with excellent accuracy, precision, and reproducibility was as low as 0.58ng/ml. The good correlation between the easy to use all-in-one dual-aptasensor and conventional enzyme-linked immunosorbent assay (ELISA), operated with time consuming procedures (e.g., long incubations and multiple washings), indicates that the rapid all-in-one dual-aptasensor can be applied as a novel clinical tool for the early diagnosis of breast cancer.
authors.affiliation: Luminescent MD, LLC, Hagerstown, MD 21742, United States.
authors.forename: Harriet
authors.initials: H
authors.lastname: Khang
authors.affiliation: Luminescent MD, LLC, Hagerstown, MD 21742, United States.
authors.forename: Kelly
authors.initials: K
authors.lastname: Cho
authors.affiliation: Luminescent MD, LLC, Hagerstown, MD 21742, United States.
authors.forename: Stephanie
authors.initials: S
authors.lastname: Chong
authors.affiliation: Luminescent MD, LLC, Hagerstown, MD 21742, United States. Electronic address: jhlee@luminescentmd.com.
authors.forename: Ji Hoon
authors.initials: JH
authors.lastname: Lee
mesh_terms: D052157:Aptamers, Nucleotide / Q000737:chemistry*; D015374:Biosensing Techniques; D002272:Carcinoembryonic Antigen / Q000737:chemistry / Q000302:isolation & purification*; D021881:DNA, Catalytic / Q000737:chemistry; D055088:Early Detection of Cancer; D054856:G-Quadruplexes; D006427:Hemin / Q000737:chemistry / Q000302:isolation & purification*; D006801:Humans; D006861:Hydrogen Peroxide / Q000737:chemistry; D007093:Imidazoles / Q000737:chemistry; D010078:Oxazines / Q000737:chemistry
chemical_list: C554599:1,1'-oxalyldiimidazole; D052157:Aptamers, Nucleotide; D002272:Carcinoembryonic Antigen; D021881:DNA, Catalytic; D007093:Imidazoles; D010078:Oxazines; C014180:resorufin; D006427:Hemin; D006861:Hydrogen Peroxide
keywords: 1,1′-oxalyldiimidazole chemiluminescence (ODI-CL); Aptasensor; Carcinoembryonic antigen (CEA); Dual aptamer; Dual-aptasensor; G-quadruplex DNAzyme