title: [Role of pannexin 1 channels in cisplatin-induced apoptosis in I-10 cells].
abstract: OBJECTIVE
  To investigate the role of pannexin 1 channels in cisplatin-induced apoptosis in I-10 cells and the mechanisms.
  
  
  METHODS
  MTT assay was used to assess the cytotoxicity of cisplatin (DDP) in I-10 cells. Annexin V/PI double staining and Hoechst 33258 fluorescence staining were employed to detect early- and late-stage apoptosis of the cells, respectively. Extracellular ATP level and intracellular IP3 level in the cells were detected using commercial detection kits.
  
  
  RESULTS
  I-10 cells exposed to both CBX (a pannexin 1 channel inhibitor) and DDP showed a higher cell viability compared with the cells exposed to DDP alone (P<0.01). CBX significantly decreased cisplatin-induced early-stage apoptosis (P<0.001) and late-stage apoptosis (P<0.01), and cause obvious reductions in extracellular ATP and intracellular IP3 levels during cisplatin-induced apoptosis (P<0.05).
  
  
  CONCLUSIONS
  Pannexin 1 channels participate in cisplatin-induced apoptosis in I-10 cells possibly through the ATP/IP3 pathway.
authors.affiliation: Department of Pharmacology, Faculty of Pharmacy, Bengbu Medical College, Bengbu 233030, China.E-mail: dc_wujianfeng@163.com.
authors.forename: Jian-Feng
authors.initials: JF
authors.lastname: Wu
authors.forename: Shu-Ying
authors.initials: SY
authors.lastname: Dong
authors.forename: Dan-Dan
authors.initials: DD
authors.lastname: Wu
authors.forename: Yin-Ling
authors.initials: YL
authors.lastname: Chen
authors.forename: Xu-Hui
authors.initials: XH
authors.lastname: Tong
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D002470:Cell Survival; D002945:Cisplatin / Q000494:pharmacology*; D017630:Connexins / Q000502:physiology*; D006801:Humans; D009419:Nerve Tissue Proteins / Q000502:physiology*
chemical_list: D000970:Antineoplastic Agents; D017630:Connexins; D009419:Nerve Tissue Proteins; C492764:PANX1 protein, human; D002945:Cisplatin