title: Investigating the Influence of MoS2 Nanosheets on E. coli from Metabolomics Level.
abstract: Molybdenum disulfide, a type of two-dimensional layered material with unique properties, has been widely used in many fields. However, an exact understanding of its toxicity remains elusive, let alone its effects on the environmental microbial community. In this study, we utilized metabolomics technology to explore the effects of different concentrations of molybdenum disulfide nanosheets on Escherichia coli for the first time. The results showed that with increasing concentration of molybdenum disulfide nanosheets, the survival rate of Escherichia coli was decreased and the release of lactic dehydrogenase was increased. At the same time, intracellular concentrations of reactive oxygen species were dramatically increased. In addition, metabolomics analysis showed that high concentrations of molybdenum disulfide nanosheets (100, 1000 μg/mL) could significantly affect the metabolic profile of Escherichia coli, including glycine, serine and threonine metabolism, protein biosynthesis, urea cycle and pyruvate metabolism. These results will be beneficial for molybdenum disulfide toxicity assessment and further applications.
authors.affiliation: College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing, China.
authors.forename: Na
authors.initials: N
authors.lastname: Wu
authors.affiliation: Jiangsu National Synergetic Innovation Center for Advanced Materials (SICAM), Nanjing, China.
authors.forename: Yadong
authors.initials: Y
authors.lastname: Yu
authors.affiliation: College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Li
authors.affiliation: College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing, China.
authors.forename: Xiaojun
authors.initials: X
authors.lastname: Ji
authors.affiliation: College of food science and light industry, Nanjing Tech University, Nanjing, China.
authors.forename: Ling
authors.initials: L
authors.lastname: Jiang
authors.affiliation: College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing, China.
authors.forename: Jiajun
authors.initials: J
authors.lastname: Zong
authors.affiliation: School of Pharmaceutical Sciences, Nanjing Tech University, Nanjing, China.
authors.forename: He
authors.identifier: http://orcid.org/0000-0003-4670-9018
authors.initials: H
authors.lastname: Huang
mesh_terms: D016000:Cluster Analysis; D004220:Disulfides / Q000008:administration & dosage* / Q000737:chemistry; D004734:Energy Metabolism / Q000187:drug effects; D004926:Escherichia coli / Q000187:drug effects* / Q000378:metabolism* / Q000648:ultrastructure; D005285:Fermentation; D008401:Gas Chromatography-Mass Spectrometry; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D053858:Metabolic Networks and Pathways; D055442:Metabolome; D055432:Metabolomics / Q000379:methods; D050296:Microbial Viability / Q000187:drug effects; D008982:Molybdenum / Q000008:administration & dosage* / Q000737:chemistry; D049329:Nanostructures / Q000737:chemistry / Q000648:ultrastructure; D018384:Oxidative Stress; D017382:Reactive Oxygen Species
chemical_list: D004220:Disulfides; D017382:Reactive Oxygen Species; D008982:Molybdenum; D007770:L-Lactate Dehydrogenase; C082964:molybdenum disulfide