title: Citrus psorosis virus coat protein-derived hairpin construct confers stable transgenic resistance in citrus against psorosis A and B syndromes.
abstract: Citrus psorosis virus (CPsV) is the causal agent of psorosis, a serious and widespread citrus disease. Two syndromes of psorosis, PsA and PsB, have been described. PsB is the most aggressive and rampant form. Previously, we obtained Pineapple sweet orange plants transformed with a hairpin construct derived from the CPsV coat protein gene (ihpCP). Some of these plants were resistant to CPsV 90-1-1, a PsA isolate homologous to the transgene. In this study, we found that expression of the ihpCP transgene and siRNA production in lines ihpCP-10 and -15 were stable with time and propagation. In particular, line ihpCP-15 has been resistant for more than 2 years, even after re-inoculation. The ihpCP plants were also resistant against a heterologous CPsV isolate that causes severe PsB syndrome. Line ihpCP-15 manifested complete resistance while line ihpCP-10 was tolerant to the virus, although with variable behaviour, showing delay and attenuation in PsB symptoms. These lines are promising for a biotech product aimed at eradicating psorosis.
authors.affiliation: Instituto de Biotecnología y Biología Molecular, CCT-La Plata, CONICET - UNLP, calles 47 y 115, 1900, La Plata, Buenos Aires, Argentina.
authors.forename: A
authors.initials: A
authors.lastname: De Francesco
authors.affiliation: Estación Experimental Agropecuaria, INTA Concordia, Ruta Provincial 22 y vías del Ferrocarril, 3200, Concordia, Entre Ríos, Argentina.
authors.forename: N
authors.initials: N
authors.lastname: Costa
authors.affiliation: Instituto de Biotecnología y Biología Molecular, CCT-La Plata, CONICET - UNLP, calles 47 y 115, 1900, La Plata, Buenos Aires, Argentina. garcia_m@biol.unlp.edu.ar.
authors.forename: M L
authors.initials: ML
authors.lastname: García
mesh_terms: D036022:Capsid Proteins / Q000235:genetics*; D002957:Citrus / Q000235:genetics* / Q000254:growth & development / Q000821:virology; D060467:Disease Resistance / Q000235:genetics*; D010935:Plant Diseases / Q000235:genetics / Q000821:virology; D010942:Plant Viruses / Q000235:genetics / Q000472:pathogenicity; D030821:Plants, Genetically Modified / Q000235:genetics* / Q000254:growth & development / Q000821:virology; D012328:RNA Viruses / Q000235:genetics; D034741:RNA, Small Interfering / Q000235:genetics
chemical_list: D036022:Capsid Proteins; D034741:RNA, Small Interfering
keywords: Citrus psorosis virus; Hairpin-RNA; Psorosis A; Psorosis B; Transgenic sweet orange; Virus resistance