title: To each his own: no evidence of gyrodactylid parasite host switches from invasive poeciliid fishes to Goodea atripinnis Jordan (Cyprinodontiformes: Goodeidae), the most dominant endemic freshwater goodeid fish in the Mexican Highlands.
abstract: Goodeid topminnows are live-bearing fishes endemic to the Mexican Highlands (Mesa Central, MC). Unfortunately, in the MC, environmental degradation and introduced species have pushed several goodeid species to the brink of extinction. Invasive fishes can introduce exotic parasites, and the most abundant goodeid, blackfin goodea Goodea atripinnis Jordan, is parasitised by six exotic helminths. Poeciliids are widely dispersed invasive fishes, which exert negative ecological effects on goodeids. Poeciliids host several species of the monogenean genus Gyrodactylus von Nordmann, 1832, including pathogenic, invasive parasites. Here, we looked for evidence of Gyrodactylus species switching hosts from poeciliids to goodeids.
  
  
  
  Fish were collected in rivers draining the MC into both sides of the continental divide. Hosts were screened for gyrodactylid parasites in localities where G. atripinnis and poeciliids occurred sympatrically. Gyrodactylus specimens were characterised morphologically (attachment apparatus) and molecularly (internal transcribed spacer region, ITS). A Bayesian phylogenetic tree using ITS sequences established relationships between gyrodactylids collected from goodeid fishes and those from parasites infecting poeciliids.
  
  
  
  Gyrodactylids were collected from G. atripinnis in six localities on both sides of the watershed where exotic poeciliids occurred sympatrically. Morphological and molecular analyses indicated the presence of four undescribed species of Gyrodactylus infecting this goodeid host. Gyrodactylus tomahuac n. sp., the most abundant and geographically widespread species, is described here. The other three Gyrodactylus spp. are not described, but their ITS sequences are used as molecular data presented here, are the only available for gyrodactylids infecting goodeid fishes. Morphological and molecular data suggest that two distinct groups of gyrodactylids infect goodeids, one of which shares a common ancestor with gyrodactylids parasitizing poeciliids.
  
  
  
  No evidence was found of gyrodactylids switching hosts from invasive poeciliids to endemic goodeids, nor vice versa. Moreover, considering that G. atripinnis is known to host both Gyrodactylus lamothei Mendoza-Palmero, Sereno-Uribe & Salgado-Maldonado, 2009 and Gyrodactylus mexicanus Mendoza-Palmero, Sereno-Uribe & Salgado-Maldonado, 2009, with the addition of G. tomahuac n. sp. and the three undescribed Gyrodactylus spp. reported, at least six gyrodactylids may infect this host. This would make monogeneans the second most abundant parasite group infecting G. atripinnis, which to date is known to harbour 22 helminth species: nine digeneans, five nematodes, four cestodes, three monogeneans and one acanthocephalan.
authors.affiliation: Instituto de Ecología, A.C., Red de Biología Evolutiva, Km 2.5 Ant. Carretera a Coatepec, Xalapa, Veracruz, 91070, Mexico. miguel.rubio@inecol.mx.
authors.forename: Miguel
authors.initials: M
authors.lastname: Rubio-Godoy
authors.affiliation: Instituto de Ecología, A.C., Red de Biología Evolutiva, Km 2.5 Ant. Carretera a Coatepec, Xalapa, Veracruz, 91070, Mexico.
authors.forename: Ulises
authors.initials: U
authors.lastname: Razo-Mendivil
authors.affiliation: Instituto de Ecología, A.C., Red de Biología Evolutiva, Km 2.5 Ant. Carretera a Coatepec, Xalapa, Veracruz, 91070, Mexico.
authors.forename: Adriana
authors.initials: A
authors.lastname: García-Vásquez
authors.affiliation: Ross University School of Veterinary Medicine, PO Box 334, Basseterre, St. Kitts And Nevis.
authors.forename: Mark A
authors.initials: MA
authors.lastname: Freeman
authors.affiliation: Institute of Aquaculture, Faculty of Natural Sciences, University of Stirling, FK9 4LA, Stirling, UK.|Fish Vet Group Asia Limited, 99/386, Chaengwattana Building, Moo 2, Chaengwattana Rd., Kwaeng Toongsonghong, Khet Laksi, Bangkok, 10210, Thailand.
authors.forename: Andrew P
authors.initials: AP
authors.lastname: Shinn
authors.affiliation: Institute of Aquaculture, Faculty of Natural Sciences, University of Stirling, FK9 4LA, Stirling, UK.
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Paladini
mesh_terms: D000818:Animals; D002590:Cestode Infections / Q000469:parasitology / Q000635:transmission / Q000662:veterinary*; D003532:Cyprinodontiformes / Q000469:parasitology*; D005260:Female; D005393:Fish Diseases / Q000469:parasitology* / Q000635:transmission*; D005618:Fresh Water; D006790:Host-Parasite Interactions; D058865:Introduced Species; D008297:Male; D008800:Mexico; D010986:Platyhelminths / Q000302:isolation & purification*; D011036:Poecilia / Q000469:parasitology*; D013045:Species Specificity
keywords: Enemy release hypothesis; Gyrodactylus lamothei; Gyrodactylus tomahuac; ITS; Invasive species; Mexico; Monogenea