title: Label-free piezoelectric biosensor for prognosis and diagnosis of Systemic Lupus Erythematosus.
abstract: An autoantigen piezoelectric sensor to quantify specific circulating autoantibodies in human serum is developed. The sensor consisted on a quartz crystal microbalance with dissipation monitoring (QCM-D) where TRIM21 and TROVE2 autoantigens were covalently immobilized, allowing the selective determination of autoantibodies for diagnosis and prognosis of Systemic Lupus Erythematosus (SLE). The sensitivity of the biosensor, measured as IC50value, was 1.51U/mL and 0.32U/mL, for anti-TRIM21 and anti-TROVE2 circulating autoantibodies, respectively. The sensor is also able to establish a structural interaction fingerprint pattern or profile of circulating autoantibodies, what allows scoring accurately SLE patients. Furthermore, a statistical association of global disease activity with TRIM21-TROVE2 interaction was found (n=130 lupic patient samples, p-value=0.0413). The performances of the biosensor were compared with standard ELISA and multiplex DVD-array high-throughput screening assays, corroborating the viability of piezoelectric biosensor as a cost-effective in vitro assay for the early detection, monitoring or treatment of rare diseases.
authors.affiliation: Instituto Interuniversitario de Investigación de Reconocimiento Molecular y Desarrollo Tecnológico (IDM-Departamento de Química), Universitat Politècnica de València, Camino de Vera s/n, 46022 Valencia, Spain.
authors.forename: Noelle M
authors.initials: NM
authors.lastname: do Nascimento
authors.affiliation: Departamento de Química-Física, Universitat de València, C/Dr. Moliner 50, 46100 Burjassot, Spain.
authors.forename: Augusto
authors.initials: A
authors.lastname: Juste-Dolz
authors.affiliation: Servicio de Reumatología, Hospital Universitario y Politécnico La Fe, Avenida de Fernando Abril Martorell no 106, 46026 Valencia, Spain.
authors.forename: Elena
authors.initials: E
authors.lastname: Grau-García
authors.affiliation: Servicio de Reumatología, Hospital Universitario y Politécnico La Fe, Avenida de Fernando Abril Martorell no 106, 46026 Valencia, Spain.
authors.forename: Jose A
authors.initials: JA
authors.lastname: Román-Ivorra
authors.affiliation: Instituto Interuniversitario de Investigación de Reconocimiento Molecular y Desarrollo Tecnológico (IDM-Departamento de Química), Universitat Politècnica de València, Camino de Vera s/n, 46022 Valencia, Spain.
authors.forename: Rosa
authors.initials: R
authors.lastname: Puchades
authors.affiliation: Instituto Interuniversitario de Investigación de Reconocimiento Molecular y Desarrollo Tecnológico (IDM-Departamento de Química), Universitat Politècnica de València, Camino de Vera s/n, 46022 Valencia, Spain.
authors.forename: Angel
authors.initials: A
authors.lastname: Maquieira
authors.affiliation: Instituto Interuniversitario de Investigación de Reconocimiento Molecular y Desarrollo Tecnológico (IDM-Departamento de Química), Universitat Politècnica de València, Camino de Vera s/n, 46022 Valencia, Spain. Electronic address: smorais@qim.upv.es.
authors.forename: Sergi
authors.initials: S
authors.lastname: Morais
authors.affiliation: Departamento de Química-Física, Universitat de València, C/Dr. Moliner 50, 46100 Burjassot, Spain. Electronic address: David.Gimenez-Romero@uv.es.
authors.forename: David
authors.initials: D
authors.lastname: Gimenez-Romero
mesh_terms: D001323:Autoantibodies / Q000097:blood* / Q000276:immunology; D001324:Autoantigens / Q000276:immunology*; D015374:Biosensing Techniques / Q000295:instrumentation*; D006801:Humans; D008180:Lupus Erythematosus, Systemic / Q000097:blood* / Q000175:diagnosis* / Q000276:immunology; D011379:Prognosis; D058525:Quartz Crystal Microbalance Techniques / Q000295:instrumentation*; D020733:RNA, Small Cytoplasmic / Q000276:immunology*; D012261:Ribonucleoproteins / Q000276:immunology*; D012680:Sensitivity and Specificity; D000099207:SS-A Antigen
chemical_list: D001323:Autoantibodies; D001324:Autoantigens; D020733:RNA, Small Cytoplasmic; D012261:Ribonucleoproteins; D000099207:SS-A Antigen; C116006:RO60 protein, human
keywords: Diagnosis; Dissipation monitoring; Immunosensor; Interaction fingerprint; Quartz crystal microbalance; Systemic Lupus Erythematosus