title: Investigation of CD40 gene rs4810485 and rs1883832 mutations in patients with recurrent aphthous stomatitis.
abstract: OBJECTIVE
  Recurrent aphthous stomatitis (RAS) is a common painful disorder affecting oral health, mucosa and overall quality of life. The etiopathogenesis of RAS remains unclear. RAS shows a large genetic diversity among the patients. In present study, we investigated whether CD40 gene rs4810485 and rs1883832 are associated with RAS and its clinical findings in Turkish patients.
  
  
  METHODS
  Genomic DNA obtained from 387 individuals (160 patients with RAS and 227 healthy controls) were used in the study. CD40 gene rs4810485 and rs1883832 mutations were determined by using polymerase chain reaction with the specific primers.
  
  
  RESULTS
  There was no statistically significant difference between the groups with respect to genotype and allele distribution (p>0.05, OR 0.94, 95% CI 0.70-1.28, OR 1.01 95% CI 0.75-1.37, respectively). Additionally, there was no statistically significant difference in the combined genotype analysis of CD40 gene rs4810485 and rs1883832 mutations (p>0.05).
  
  
  CONCLUSIONS
  According to our results, we found that CD40 gene mutations are not associated with RAS. We are convinced that CD40 gene mutations do not predispose to develop RAS in Turkish population. To our knowledge, this is the first study regarding CD40 gene rs4810485 and rs1883832 mutations investigated in RAS patients.
authors.affiliation: Gaziosmanpasa University, Faculty of Medicine, Department of Medical Biology, Tokat, Turkey. Electronic address: serbulent.yigit@gmail.com.
authors.forename: Serbulent
authors.initials: S
authors.lastname: Yigit
authors.affiliation: Ahi Evran University, School of Health, Kirsehir, Turkey. Electronic address: akintekcan@hotmail.com.
authors.forename: Akin
authors.initials: A
authors.lastname: Tekcan
authors.affiliation: Gaziosmanpasa University, Faculty of Medicine, Department of Medical Biology, Tokat, Turkey.
authors.forename: Aydin
authors.initials: A
authors.lastname: Rustemoglu
authors.affiliation: Gaziosmanpasa University, Faculty of Dentistry, Department of Maxillofacial Surgery, Tokat, Turkey.
authors.forename: Mehmet Kemal
authors.initials: MK
authors.lastname: Tumer
authors.affiliation: Yıldırım Beyazıt University, Faculty of Medicine, Department of Dermatovenerology, Ankara, Turkey.
authors.forename: Goknur
authors.initials: G
authors.lastname: Kalkan
authors.affiliation: Gaziosmanpasa University, Faculty of Dentistry, Department of Prosthodontics, Tokat, Turkey.
authors.forename: Kaan
authors.initials: K
authors.lastname: Yerliyurt
mesh_terms: D000328:Adult; D000483:Alleles; D019013:CD40 Antigens / Q000235:genetics*; D016022:Case-Control Studies; D005260:Female; D015870:Gene Expression; D005787:Gene Frequency; D015894:Genome, Human; D005838:Genotype; D006801:Humans; D008297:Male; D008875:Middle Aged; D009154:Mutation; D019226:Oral Ulcer / Q000235:genetics / Q000503:physiopathology; D012150:Polymorphism, Restriction Fragment Length; D013281:Stomatitis, Aphthous / Q000150:complications / Q000235:genetics*; D014421:Turkey
chemical_list: D019013:CD40 Antigens
keywords: CD40 gene; Mutations; RAS