title: Resonant nanopillars arrays for label-free biosensing.
abstract: In our previous work we demonstrated for the first time, to the best of our knowledge, the experimental capability of resonant nanopillars (R-NP) arrays as biochemical transducers. In this Letter, we provide evidence of the capability and suitability of R-NP arrays on a chip to function as label-free optical multiplexed biosensors. R-NP are based on Si3N4/SiO2Bragg reflectors with a cavity of SiO2. In order to demonstrate the biosensing performance, R-NP were biofunctionalized by the immobilization of IgG antibodies acting as a bioreceptor. This immobilization was carried out through the silanization of the pillars sensing surface with APTMS (3-aminopropyltrimethoxysilane). R-NP were integrated in eight different sensing arrays on a quartz surface chip. An optical fiber bundle monitored each sensing array vertically and independently after each biofunctionalization step, and subsequently after every recognition event of increasing concentrations of anti-IgGs. The results report a novel multiplexed optical biosensor made of eight sensing arrays on a chip with promising performance and yield.
authors.forename: Ana L
authors.initials: AL
authors.lastname: Hernández
authors.forename: Rafael
authors.initials: R
authors.lastname: Casquel
authors.forename: Miguel
authors.initials: M
authors.lastname: Holgado
authors.forename: Iñaki
authors.initials: I
authors.lastname: Cornago
authors.forename: Fátima
authors.initials: F
authors.lastname: Fernández
authors.forename: Paula
authors.initials: P
authors.lastname: Ciaurriz
authors.forename: Francisco J
authors.initials: FJ
authors.lastname: Sanza
authors.forename: Beatriz
authors.initials: B
authors.lastname: Santamaría
authors.forename: María V
authors.initials: MV
authors.lastname: Maigler
authors.forename: María Fe
authors.initials: MF
authors.lastname: Laguna
mesh_terms: D049329:Nanostructures; D020411:Oligonucleotide Array Sequence Analysis; D055100:Optical Fibers; D012822:Silicon Dioxide; D014159:Transducers
chemical_list: D012822:Silicon Dioxide