title: miR-520e regulates cell proliferation, apoptosis and migration in breast cancer.
abstract: Previous studies have indicated that the deregulation of microRNAs contributes to tumorigenesis. Misregulation of microRNA-520e (miR-520e) has been observed in various types of cancer. However, the expression profile and biological function of miR-520e in breast cancer remains largely unknown. The present study demonstrated that miR-520e expression was significantly increased in breast cancer tissues compared with adjacent non-cancerous breast tissues in 21 patients, as revealed by reverse transcription-quantitative polymerase chain reaction. Furthermore, the proliferation capacity of breast cancer cells was markedly enhanced by the introduction of miR-520ein vitrousing a cell counting kit-8 assay. The present study also revealed that the overexpression of miR-520e could suppress breast cancer cell apoptosis, revealed using Annexin V/propidium iodide double staining and flow cytometry analysis. In addition, the ectopic expression of miR-520e promoted the migration of breast cancer cellsin vitro, as demonstrated by a Transwell assay. Overall, the findings of the present study highlight an important role for miR-520e in breast cancer development and in the molecular etiology of breast cancer, which indicates the potential application of miR-520e in cancer therapy.
authors.affiliation: Central Laboratory, Peking University Shenzhen Hospital, Shenzhen, Guangdong 518036, P.R. China; Department of Graduate Studies, Shantou University Medical College, Shantou, Guangdong 515041, P.R. China.
authors.forename: Ming
authors.initials: M
authors.lastname: Yi
authors.affiliation: Central Laboratory, Peking University Shenzhen Hospital, Shenzhen, Guangdong 518036, P.R. China.
authors.forename: Minghua
authors.initials: M
authors.lastname: Li
authors.affiliation: Central Laboratory, Peking University Shenzhen Hospital, Shenzhen, Guangdong 518036, P.R. China.
authors.forename: Xia
authors.initials: X
authors.lastname: Long
authors.affiliation: Central Laboratory, Peking University Shenzhen Hospital, Shenzhen, Guangdong 518036, P.R. China.
authors.forename: Jing
authors.initials: J
authors.lastname: Ye
authors.affiliation: Central Laboratory, Peking University Shenzhen Hospital, Shenzhen, Guangdong 518036, P.R. China.
authors.forename: Junwei
authors.initials: J
authors.lastname: Cui
authors.affiliation: Central Laboratory, Peking University Shenzhen Hospital, Shenzhen, Guangdong 518036, P.R. China.
authors.forename: Wei
authors.initials: W
authors.lastname: Wei
authors.affiliation: Central Laboratory, Peking University Shenzhen Hospital, Shenzhen, Guangdong 518036, P.R. China.
authors.forename: Huijuan
authors.initials: H
authors.lastname: Wan
authors.affiliation: Central Laboratory, Peking University Shenzhen Hospital, Shenzhen, Guangdong 518036, P.R. China.
authors.forename: Meijun
authors.initials: M
authors.lastname: Yin
authors.affiliation: Department of Biochemistry and Molecular Biology, Zhuhai Campus Zunyi Medical University, Zhuhai, Guangdong 519041, P.R. China.
authors.forename: Shuying
authors.initials: S
authors.lastname: Gao
authors.affiliation: Central Laboratory, Peking University Shenzhen Hospital, Shenzhen, Guangdong 518036, P.R. China; Department of Graduate Studies, Shantou University Medical College, Shantou, Guangdong 515041, P.R. China.
authors.forename: Zhengming
authors.initials: Z
authors.lastname: Su
authors.affiliation: Central Laboratory, Peking University Shenzhen Hospital, Shenzhen, Guangdong 518036, P.R. China.
authors.forename: Fangting
authors.initials: F
authors.lastname: Zhang
keywords: apoptosis; breast cancer; miRNA-520e; migration; proliferation