title: Self-Fertility in a Cultivated Diploid Potato Population Examined with the Infinium 8303 Potato Single-Nucleotide Polymorphism Array.
abstract: Within a population of F hybrids between two genotypes ( L. Group Phureja DM 1-3 516 R44 [DM] and L. Group Tuberosum RH89-039-16 [RH]) used in the potato genome sequencing project, we observed fruit set after self-pollination on many plants. Examination of pollen tube growth in self-fertile and self-unfruitful F plants after controlled self-pollinations revealed no difference in the ability of pollen tubes to reach the ovary. To identify genomic regions linked with self-fertility, we genotyped the F population using a genome-wide single-nucleotide polymorphism (SNP) array. Polymorphic and robust SNPs were analyzed to identify allelic states segregating with the self-fertile phenotype. All 88 highly significant SNPs occurred on chromosome 12. Seeds obtained after self-pollination of self-fertile individuals were used to advance the population for four generations. Genotyping 46 self-fruitful and 46 self-unfruitful S plants on the Infinium 8303 Potato SNP array revealed eight SNPs segregating with self-fertility on chromosomes 4, 9, 11, and 12. Three times more heterozygosity than expected was found in the S generation. Estimates of heterozygosity were influenced by copy number variation (CNV) in the potato genome leading to spurious heterozygous genotyping calls. Some spurious heterozygosity could be removed by application of a CNV filter developed from alignment of additional monoploid potato genomic sequence to the DM reference genome. The genes responsible for fruit set in self-fertile plants in the F generation were restricted to chromosome 12, whereas new genomic regions contributed to the ability of S plants to set fruit after self-pollination.
authors.forename: Brenda A
authors.initials: BA
authors.lastname: Peterson
authors.forename: Sarah H
authors.initials: SH
authors.lastname: Holt
authors.forename: F Parker E
authors.initials: FP
authors.lastname: Laimbeer
authors.forename: Andreas G
authors.initials: AG
authors.lastname: Doulis
authors.forename: Joseph
authors.initials: J
authors.lastname: Coombs
authors.forename: David S
authors.initials: DS
authors.lastname: Douches
authors.forename: Michael A
authors.initials: MA
authors.lastname: Hardigan
authors.forename: C Robin
authors.initials: CR
authors.lastname: Buell
authors.forename: Richard E
authors.initials: RE
authors.lastname: Veilleux
mesh_terms: D002874:Chromosome Mapping; D056915:DNA Copy Number Variations; D004171:Diploidy; D005298:Fertility / Q000235:genetics; D018745:Genome, Plant / Q000235:genetics*; D005838:Genotype; D020411:Oligonucleotide Array Sequence Analysis; D020641:Polymorphism, Single Nucleotide; D011198:Solanum tuberosum / Q000235:genetics*