title: Microvirga ossetica sp. nov., a species of rhizobia isolated from root nodules of the legume species Vicia alpestris Steven.
abstract: Gram-stain-negative strains V5/3MT, V5/5K, V5/5M and V5/13 were isolated from root nodules of Vicia alpestris plants growing in the North Ossetia region (Caucasus). Sequencing of the partial 16S rRNA gene (rrs) and four housekeeping genes (dnaK, gyrB, recA and rpoB) showed that the isolates from V. alpestris were most closely related to the species Microvirga zambiensis (order Rhizobiales, family Methylobacteriaceae) which was described for the single isolate from root nodule of Listia angolensis growing in Zambia. Sequence similarities between the Microvirga-related isolates and M. zambiensis WSM3693T ranged from 98.5 to 98.7 % for rrs and from 79.7 to 95.8 % for housekeeping genes. Cellular fatty acids of the isolates V5/3MT, V5/5K, V5/5M and V5/13 included important amounts of C18 : 1ω7c (54.0-67.2 %), C16 : 0 (6.0-7.8 %), C19 : 0 cyclo ω8c (3.1-10.2 %), summed feature 2 (comprising one or more of iso-C16 : 1 I, C14 : 0 3-OH and unknown ECL 10.938, 5.8-22.5 %) and summed feature 3 (comprising C16 : 1ω7c and/or iso-C15 : 02-OH, 2.9-4.0 %). DNA-DNA hybridization between the isolate V5/3MT and M. zambiensis WSM3693T revealed DNA-DNA relatedness of 35.3 %. Analysis of morphological and physiological features of the novel isolates demonstrated their unique phenotypic profile in comparison with reference strains from closely related species of the genus Microvirga. On the basis of genotypic and phenotypic analysis, a novel species named Microvirga ossetica sp. nov. is proposed. The type strain is V5/3MT (=LMG 29787T=RCAM 02728T). Three additional strains of the species are V5/5K, V5/5M and V5/13.
authors.affiliation: All-Russia Research Institute for Agricultural Microbiology (ARRIAM), sh. Podbelskogo 3, 196608, St.-Petersburg, Russian Federation.
authors.forename: Vera I
authors.initials: VI
authors.lastname: Safronova
authors.affiliation: All-Russia Research Institute for Agricultural Microbiology (ARRIAM), sh. Podbelskogo 3, 196608, St.-Petersburg, Russian Federation.
authors.forename: Irina G
authors.initials: IG
authors.lastname: Kuznetsova
authors.affiliation: All-Russia Research Institute for Agricultural Microbiology (ARRIAM), sh. Podbelskogo 3, 196608, St.-Petersburg, Russian Federation.
authors.forename: Anna L
authors.initials: AL
authors.lastname: Sazanova
authors.affiliation: All-Russia Research Institute for Agricultural Microbiology (ARRIAM), sh. Podbelskogo 3, 196608, St.-Petersburg, Russian Federation.
authors.forename: Andrey A
authors.initials: AA
authors.lastname: Belimov
authors.affiliation: All-Russia Research Institute for Agricultural Microbiology (ARRIAM), sh. Podbelskogo 3, 196608, St.-Petersburg, Russian Federation.
authors.forename: Evgeny E
authors.initials: EE
authors.lastname: Andronov
authors.affiliation: All-Russia Research Institute for Agricultural Microbiology (ARRIAM), sh. Podbelskogo 3, 196608, St.-Petersburg, Russian Federation.
authors.forename: Elizaveta R
authors.initials: ER
authors.lastname: Chirak
authors.affiliation: All-Russia Research Institute for Agricultural Microbiology (ARRIAM), sh. Podbelskogo 3, 196608, St.-Petersburg, Russian Federation.
authors.forename: Yuri S
authors.initials: YS
authors.lastname: Osledkin
authors.affiliation: All-Russia Research Institute for Agricultural Microbiology (ARRIAM), sh. Podbelskogo 3, 196608, St.-Petersburg, Russian Federation.
authors.forename: Olga P
authors.initials: OP
authors.lastname: Onishchuk
authors.affiliation: All-Russia Research Institute for Agricultural Microbiology (ARRIAM), sh. Podbelskogo 3, 196608, St.-Petersburg, Russian Federation.
authors.forename: Oksana N
authors.initials: ON
authors.lastname: Kurchak
authors.affiliation: All-Russia Research Institute for Agricultural Microbiology (ARRIAM), sh. Podbelskogo 3, 196608, St.-Petersburg, Russian Federation.
authors.forename: Alexander I
authors.initials: AI
authors.lastname: Shaposhnikov
authors.affiliation: Department of Biochemistry & Microbiology, Faculty of Sciences, Ghent University, 9000 Ghent, K.L. Ledeganckstraat 35, Belgium.
authors.forename: Anne
authors.initials: A
authors.lastname: Willems
authors.affiliation: Department of Genetics and Biotechnology, Saint Petersburg State University, 199034, St.-Petersburg, Universitetskaya Emb.7/9, Russian Federation.|All-Russia Research Institute for Agricultural Microbiology (ARRIAM), sh. Podbelskogo 3, 196608, St.-Petersburg, Russian Federation.
authors.forename: Igor A
authors.initials: IA
authors.lastname: Tikhonovich
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D005798:Genes, Bacterial; D042082:Methylobacteriaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D053204:Root Nodules, Plant / Q000382:microbiology*; D012426:Russia; D017422:Sequence Analysis, DNA; D031306:Vicia / Q000382:microbiology*
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S