title: Time before anti-Toxoplasma IgG seroconversion detection by 7 commercial assays in French pregnant women.
abstract: We assessed the ability to early detect a toxoplasmic seroconversion between 1 immunoblot (LDBIO II®) and 6 automated assays (TGS TA®, Architect®, Vidas II®, Liaison II®, Platelia®, and Elecsys®), comparing the time before anti-Toxoplasma gondii IgG detection during infection in pregnant women. From 2007 to 2015, 620 sera of 269 women were included. The median durations before positive IgG detection with Vidas II®, Liaison II®, Platelia®, and Elecsys® were significantly longer than Architect® with differential times from 11 to 28days (P<0.001). This time was significantly shortened by the use of LDBIO®, resulting in a saving of 13days (P<0.001). The detection of a positive rate of IgG with TGS TA® was as early as Architect® (P=0.105). The ability to early detect a toxoplasmic seroconversion is not equivalent between the assays and has to be considered when selecting the reagents to reduce the time to therapeutic intervention.
authors.affiliation: Service de Parasitologie-Mycologie, Hôpital Purpan, Toulouse, 31059, France.
authors.forename: Catherine
authors.initials: C
authors.lastname: Armengol
authors.affiliation: Service de Parasitologie-Mycologie, Hôpital Purpan, Toulouse, 31059, France; Pharmacochimie et Biologie Pour le Développement (PHARMA-DEV), IRD UMR 152 Université Paul Sabatier, Toulouse, 31400, France.
authors.forename: Sophie
authors.initials: S
authors.lastname: Cassaing
authors.affiliation: Service de Parasitologie-Mycologie, Hôpital Purpan, Toulouse, 31059, France.
authors.forename: Christine
authors.initials: C
authors.lastname: Roques-Malecaze
authors.affiliation: Service de Parasitologie-Mycologie, Hôpital Purpan, Toulouse, 31059, France.
authors.forename: Pamela
authors.initials: P
authors.lastname: Chauvin
authors.affiliation: Service de Parasitologie-Mycologie, Hôpital Purpan, Toulouse, 31059, France.
authors.forename: Xavier
authors.initials: X
authors.lastname: Iriart
authors.affiliation: Service de Parasitologie-Mycologie, Hôpital Purpan, Toulouse, 31059, France.
authors.forename: Antoine
authors.initials: A
authors.lastname: Berry
authors.affiliation: Service de Parasitologie-Mycologie, Hôpital Purpan, Toulouse, 31059, France; Pharmacochimie et Biologie Pour le Développement (PHARMA-DEV), IRD UMR 152 Université Paul Sabatier, Toulouse, 31400, France. Electronic address: fillaux.j@chu-toulouse.fr.
authors.forename: Judith
authors.initials: J
authors.lastname: Fillaux
mesh_terms: D000328:Adult; D000913:Antibodies, Protozoan / Q000097:blood*; D057205:Automation, Laboratory / Q000379:methods; D003955:Diagnostic Tests, Routine / Q000379:methods*; D005260:Female; D005602:France; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood*; D011247:Pregnancy; D011251:Pregnancy Complications, Infectious / Q000175:diagnosis*; D011933:Reagent Kits, Diagnostic; D000069078:Seroconversion; D013997:Time Factors; D014122:Toxoplasma / Q000276:immunology*; D014123:Toxoplasmosis / Q000175:diagnosis*
chemical_list: D000913:Antibodies, Protozoan; D007074:Immunoglobulin G; D011933:Reagent Kits, Diagnostic
keywords: Automated tests; Immunoblot; Immunodiagnosis; Pregnancy; Seroconversion; Toxoplasma gondii