title: Human adipose tissue-derived mesenchymal stem cells alleviate atopic dermatitis via regulation of B lymphocyte maturation.
abstract: Mesenchymal stem cell (MSC) has been applied for the therapy of allergic disorders due to its beneficial immunomodulatory abilities. However, the underlying mechanisms for therapeutic efficacy are reported to be diverse according to the source of cell isolation or the route of administration. We sought to investigate the safety and the efficacy of human adipose tissue-derived MSCs (hAT-MSCs) in mouse atopic dermatitis (AD) model and to determine the distribution of cells after intravenous administration. Murine AD model was established by multiple treatment of Dermatophagoides farinae. AD mice were intravenously infused with hAT-MSCs and monitored for clinical symptoms. The administration of hAT-MSCs reduced the gross and histological signatures of AD, as well as serum IgE level. hAT-MSCs were mostly detected in lung and heart of mice within 3 days after administration and were hardly detectable at 2 weeks. All of mice administered with hAT-MSCs survived until sacrifice and did not demonstrate any adverse events. Co-culture experiments revealed that hAT-MSCs significantly inhibited the proliferation and the maturation of B lymphocytes via cyclooxygenase (COX)-2 signaling. Moreover, mast cell (MC) degranulation was suppressed by hAT-MSC. In conclusion, the intravenous infusion of hAT-MSCs can alleviate AD through the regulation of B cell function.
authors.affiliation: Adult Stem Cell Research Center, College of Veterinary Medicine, Seoul National University, Seoul 08826, South Korea.|Research Institute for Veterinary Science, College of Veterinary Medicine, Seoul National University, Seoul 08826, South Korea.
authors.forename: Tae-Hoon
authors.initials: TH
authors.lastname: Shin
authors.affiliation: Adult Stem Cell Research Center, College of Veterinary Medicine, Seoul National University, Seoul 08826, South Korea.|Research Institute for Veterinary Science, College of Veterinary Medicine, Seoul National University, Seoul 08826, South Korea.
authors.forename: Byung-Chul
authors.initials: BC
authors.lastname: Lee
authors.affiliation: Adult Stem Cell Research Center, College of Veterinary Medicine, Seoul National University, Seoul 08826, South Korea.|Research Institute for Veterinary Science, College of Veterinary Medicine, Seoul National University, Seoul 08826, South Korea.
authors.forename: Soon Won
authors.initials: SW
authors.lastname: Choi
authors.affiliation: Adult Stem Cell Research Center, College of Veterinary Medicine, Seoul National University, Seoul 08826, South Korea.|Research Institute for Veterinary Science, College of Veterinary Medicine, Seoul National University, Seoul 08826, South Korea.
authors.forename: Ji-Hee
authors.initials: JH
authors.lastname: Shin
authors.affiliation: Adult Stem Cell Research Center, College of Veterinary Medicine, Seoul National University, Seoul 08826, South Korea.|Research Institute for Veterinary Science, College of Veterinary Medicine, Seoul National University, Seoul 08826, South Korea.
authors.forename: Insung
authors.initials: I
authors.lastname: Kang
authors.affiliation: Adult Stem Cell Research Center, College of Veterinary Medicine, Seoul National University, Seoul 08826, South Korea.|Research Institute for Veterinary Science, College of Veterinary Medicine, Seoul National University, Seoul 08826, South Korea.
authors.forename: Jin Young
authors.initials: JY
authors.lastname: Lee
authors.affiliation: Adult Stem Cell Research Center, College of Veterinary Medicine, Seoul National University, Seoul 08826, South Korea.|Research Institute for Veterinary Science, College of Veterinary Medicine, Seoul National University, Seoul 08826, South Korea.
authors.forename: Jae-Jun
authors.initials: JJ
authors.lastname: Kim
authors.affiliation: Biotechnology Institute, EHL-BIO Co., Ltd., Uiwang 16006, South Korea.
authors.forename: Hong-Ki
authors.initials: HK
authors.lastname: Lee
authors.affiliation: Biotechnology Institute, EHL-BIO Co., Ltd., Uiwang 16006, South Korea.
authors.forename: Jae-Eon
authors.initials: JE
authors.lastname: Jung
authors.affiliation: Biotechnology Institute, EHL-BIO Co., Ltd., Uiwang 16006, South Korea.
authors.forename: Yong-Woon
authors.initials: YW
authors.lastname: Choi
authors.affiliation: Biotechnology Institute, EHL-BIO Co., Ltd., Uiwang 16006, South Korea.
authors.forename: Sung-Hoon
authors.initials: SH
authors.lastname: Lee
authors.affiliation: Biotechnology Institute, EHL-BIO Co., Ltd., Uiwang 16006, South Korea.
authors.forename: Jin-Sang
authors.initials: JS
authors.lastname: Yoon
authors.affiliation: Biotechnology Institute, EHL-BIO Co., Ltd., Uiwang 16006, South Korea.
authors.forename: Jin-Sub
authors.initials: JS
authors.lastname: Choi
authors.affiliation: School of Medicine, Pusan National University, Busan 49241, South Korea.|Biomedical Research Institute, Pusan National University Hospital, Busan 49241, South Korea.
authors.forename: Chi-Seung
authors.initials: CS
authors.lastname: Lee
authors.affiliation: Adult Stem Cell Research Center, College of Veterinary Medicine, Seoul National University, Seoul 08826, South Korea.|School of Medicine, Pusan National University, Busan 49241, South Korea.|Biomedical Research Institute, Pusan National University Hospital, Busan 49241, South Korea.
authors.forename: Yoojin
authors.initials: Y
authors.lastname: Seo
authors.affiliation: Adult Stem Cell Research Center, College of Veterinary Medicine, Seoul National University, Seoul 08826, South Korea.|School of Medicine, Pusan National University, Busan 49241, South Korea.|Biomedical Research Institute, Pusan National University Hospital, Busan 49241, South Korea.
authors.forename: Hyung-Sik
authors.initials: HS
authors.lastname: Kim
authors.affiliation: Adult Stem Cell Research Center, College of Veterinary Medicine, Seoul National University, Seoul 08826, South Korea.|Research Institute for Veterinary Science, College of Veterinary Medicine, Seoul National University, Seoul 08826, South Korea.
authors.forename: Kyung-Sun
authors.initials: KS
authors.lastname: Kang
mesh_terms: D000273:Adipose Tissue / Q000166:cytology*; D000818:Animals; D001402:B-Lymphocytes / Q000166:cytology* / Q000502:physiology*; D002454:Cell Differentiation; D002478:Cells, Cultured; D018920:Coculture Techniques; D051546:Cyclooxygenase 2 / Q000378:metabolism; D003876:Dermatitis, Atopic / Q000276:immunology* / Q000378:metabolism* / Q000473:pathology / Q000628:therapy; D004195:Disease Models, Animal; D006801:Humans; D008297:Male; D008407:Mast Cells / Q000276:immunology / Q000378:metabolism; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism*; D051379:Mice; D015398:Signal Transduction; D053773:Transforming Growth Factor beta1 / Q000378:metabolism
chemical_list: D053773:Transforming Growth Factor beta1; D051546:Cyclooxygenase 2
keywords: B cell maturation; atopic dermatitis; distribution; mast cell degranulation; mesenchymal stem cells