title: Evaluation of Immunoglobulin G Responses toPlasmodium falciparumandPlasmodium vivaxin Malian School Children Using Multiplex Bead Assay.
abstract: Malaria serology through assaying for IgG againstPlasmodiumspp. antigens provides evidence into the infection history for an individual. The multiplex bead assay (MBA) allows for detection of IgG against multiplePlasmodiumspp., and can be especially useful in many regions wherePlasmodium falciparumis of primary clinical focus, but other species are co-endemic. Dried blood spots were collected from 805 Malian children attending 42 elementary schools in the regions of Mopti, Sikasso, Koulikoro, and Bamako capital district, and IgG assayed by MBA. As southern Mali is known to be holoendemic forP. falciparum, merozoite surface protein 1 19-kDa subunit (MSP-142) and apical membrane antigen 1 (AMA-1) antigens were included for serology against this parasite. Responses to these antigens both provided high estimates for lifetime exposure, with 730 (90%) children with IgG antibodies for MSP-142, 737 (91%) for AMA-1, and 773 (96%) positive for either or both. Also included was the antigenPlasmodium vivaxMSP-119, against which 140 (17.4%) children were found to have antibodies. Increases in antibody titers with older age were clearly seen with theP. falciparumantigens, but not with theP. vivaxantigen, likely indicating more of a sporadic, rather than sustained transmission for this species. The MBA provides effective opportunities to evaluate malaria transmission through serological analysis for multiplePlasmodiumspecies.
authors.affiliation: Division of Parasitic Diseases and Malaria, Centers for Global Health, Centers for Disease Control and Prevention, Atlanta, Georgia. wwx6@cdc.gov.
authors.forename: Eric
authors.initials: E
authors.lastname: Rogier
authors.affiliation: Division of Foodborne, Waterborne, and Environmental Diseases, National Center for Emerging and Zoonotic Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia.
authors.forename: Delynn M
authors.initials: DM
authors.lastname: Moss
authors.affiliation: Department of Environmental Health, Rollins School of Public Health, Emory University, Atlanta, Georgia.
authors.forename: Anna N
authors.initials: AN
authors.lastname: Chard
authors.affiliation: Department of Environmental Health, Rollins School of Public Health, Emory University, Atlanta, Georgia.
authors.forename: Victoria
authors.initials: V
authors.lastname: Trinies
authors.affiliation: Malaria Research and Training Center, Faculty of Medicine and Odontostomatology, University of Sciences, Techniques and Technologies of Bamako (USTTB), Bamako, Mali.
authors.forename: Seydou
authors.initials: S
authors.lastname: Doumbia
authors.affiliation: Department of Environmental Health, Rollins School of Public Health, Emory University, Atlanta, Georgia.
authors.forename: Matthew C
authors.initials: MC
authors.lastname: Freeman
authors.affiliation: Division of Parasitic Diseases and Malaria, Centers for Global Health, Centers for Disease Control and Prevention, Atlanta, Georgia.
authors.forename: Patrick J
authors.initials: PJ
authors.lastname: Lammie
mesh_terms: D000293:Adolescent; D000953:Antigens, Protozoan / Q000276:immunology; D002648:Child; D002675:Child, Preschool; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology*; D016778:Malaria, Falciparum / Q000276:immunology; D016780:Malaria, Vivax / Q000276:immunology; D008302:Mali; D008863:Microspheres; D010963:Plasmodium falciparum / Q000276:immunology*; D010966:Plasmodium vivax / Q000276:immunology*
chemical_list: D000953:Antigens, Protozoan; D007074:Immunoglobulin G