title: Biorefining of Lignocellulosic Feedstock by a Modified Ammonia Fiber Expansion Pretreatment and Enzymatic Hydrolysis for Production of Fermentable Sugars.
abstract: Wheat straw was pretreated and afterwards enzymatically hydrolyzed using a modified ammonia fiber expansion (AFEX) process under different reaction conditions to produce fermentable sugars. Instead of liquid ammonia, aqueous ammonia (25 % w/v) was used to test its influence on the sugar concentration and yield of the sugars. It is shown that a protein extraction after the pretreatment can distinctly improve the result obtained for the enzymatic hydrolysis. This modified AFEX process using aqueous ammonia represents a simpler and less expensive variant of the AFEX process usually described in literature. Thus, the described process can be used for the primary refining of lignocellulosic feedstocks in the sense of a roadmap for biorefinery.
authors.affiliation: Research Institute Biopos e.V. and BTU Cottbus-Senftenberg, Kantstraße 55, 14513, Teltow-Seehof, Germany.
authors.forename: Birgit
authors.initials: B
authors.lastname: Kamm
authors.affiliation: Biorefinery.de GmbH, Kantstraße 55, 14513, Teltow-Seehof, Germany.
authors.forename: Sebastian
authors.initials: S
authors.lastname: Leiß
authors.affiliation: Research Institute Biopos e.V. and BTU Cottbus-Senftenberg, Kantstraße 55, 14513, Teltow-Seehof, Germany.
authors.forename: Petra
authors.initials: P
authors.lastname: Schönicke
authors.affiliation: Research Institute Biopos e.V. and BTU Cottbus-Senftenberg, Kantstraße 55, 14513, Teltow-Seehof, Germany.
authors.forename: Matthias
authors.initials: M
authors.lastname: Bierbaum
mesh_terms: D000641:Ammonia / Q000737:chemistry*; D005285:Fermentation; D006026:Glycoside Hydrolases / Q000378:metabolism*; D006868:Hydrolysis; D008031:Lignin / Q000737:chemistry*; D009005:Monosaccharides / Q000737:chemistry* / Q000378:metabolism
chemical_list: D009005:Monosaccharides; C036909:lignocellulose; D000641:Ammonia; D008031:Lignin; D006026:Glycoside Hydrolases
keywords: ammonia; biomass; biorefineries; hydrolysis; sugars