title: Interaction of poly(ethylene glycol)-conjugated phospholipids with supported lipid membranes and their influence on protein adsorption.
abstract: We studied real-time interaction between poly(ethylene glycol)-conjugated phospholipids (PEG-lipids) and a supported lipid membrane by surface plasmon resonance (SPR) spectroscopy to understand dynamic behaviors of PEG-lipids on living cell membranes. Supported lipid membranes formed on a hydrophobic surface were employed as a model of living cell membrane. We prepared three kinds of PEG-lipids that carried alkyl chains of different lengths for SPR measurements and also performed fluorescence recovery after photobleaching (FRAP) to study the influence of acyl chain length on dynamics on the supported membrane. PEG-lipids were uniformly anchored to lipid membranes with high fluidity without clustering. Incorporation and dissociation rates of PEG-lipids into supported membranes strongly depended on the length of acyl chains; longer acyl chains reduced the incorporation rate and the dissociation rate of PEG-lipid. Furthermore, protein adsorption experiment with bovine serum albumin indicated that PEG modification prevented the adsorption of bovine serum albumin on such supported membrane.
authors.affiliation: Department of Reparative Materials, Institute for Frontier Medical Sciences, Kyoto University , Kyoto , Japan.
authors.forename: Toshihiro
authors.initials: T
authors.lastname: Yamamoto
authors.affiliation: Department of Reparative Materials, Institute for Frontier Medical Sciences, Kyoto University, Kyoto, Japan; Department of Bioengineering, The University of Tokyo, Tokyo, Japan.
authors.forename: Yuji
authors.initials: Y
authors.lastname: Teramura
authors.affiliation: Department of Reparative Materials, Institute for Frontier Medical Sciences, Kyoto University, Kyoto, Japan; Department of Material Chemistry, Graduate School of Engineering, Kyoto University, Kyoto, Japan.
authors.forename: Toru
authors.initials: T
authors.lastname: Itagaki
authors.affiliation: Department of Reparative Materials, Institute for Frontier Medical Sciences, Kyoto University , Kyoto , Japan.
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Arima
authors.affiliation: Department of Reparative Materials, Institute for Frontier Medical Sciences, Kyoto University , Kyoto , Japan.
authors.forename: Hiroo
authors.initials: H
authors.lastname: Iwata
keywords: 212 Surface and interfaces; 30 Bio-inspired and biomedical materials; Poly(ethylene glycol)-conjugated phospholipid (PEG-lipids); hydrophobic interaction; membrane fluidity; supported lipid membrane; surface modification