title: Effect of Lycopene and Rosmarinic Acid on Gentamicin Induced Renal Cortical Oxidative Stress, Apoptosis, and Autophagy in Adult Male Albino Rat.
abstract: Gentamicin nephrotoxicity accounts for 10%-15% of all cases of acute renal failure. Several natural antioxidants were found to be effective against drug-induced toxicity. The possible protective effects of lycopene (Lyc) and rosmarinic acid (RA) alone or combined on gentamicin (Gen) induced renal cortical oxidative stress, apoptosis, and autophagy were evaluated. Sixty-three rats were randomly divided into seven groups named: control, group II received RA 50 mg/kg/day, group III received Lyc 4 mg/kg/day, group IV received Gen 100 mg/kg/day, group V (RA + Gen), group VI (Lyc + Gen), and group VII (RA + Lyc + Gen). At the end of the experiment, kidney functions were estimated then the kidneys were sampled for histopathological, immunohistochemistry, and biochemical studies. Administration of rosmarinic acid and lycopene decreased elevated serum creatinine, blood urea nitrogen, renal malondialdehyde and immunoexpression of the proapoptotic protein (Bax), autophagic marker protein (LC3/B), and inducible nitric oxide synthase (iNOS) induced by gentamicin. They increased reduced glutathione, glutathione peroxidase, superoxide dismutase, and immunoexpression of the antiapoptotic protein (Bcl2). They also improved the histopathological changes induced by gentamicin. The combination therapy of rosmarinic acid and lycopene shows better protective effects than the corresponding monotherapy. Anat Rec, 300:1137-1149, 2017. © 2016 Wiley Periodicals, Inc.
authors.affiliation: Histology department Faculty of Medicine, Tanta University, Tanta, Egypt.
authors.forename: Naglaa A
authors.initials: NA
authors.lastname: Bayomy
authors.affiliation: Histology department Faculty of Medicine, Tanta University, Tanta, Egypt.
authors.forename: Reda H
authors.initials: RH
authors.lastname: Elbakary
authors.affiliation: Histology department Faculty of Medicine, Tanta University, Tanta, Egypt.
authors.forename: Marwa A A
authors.identifier: http://orcid.org/0000-0002-8854-8198
authors.initials: MAA
authors.lastname: Ibrahim
authors.affiliation: Pharmacology department Faculty of Medicine, Suez Canal University, Ismalia, Egypt.
authors.forename: Eman Z
authors.initials: EZ
authors.lastname: Abdelaziz
mesh_terms: D058186:Acute Kidney Injury / Q000139:chemically induced / Q000517:prevention & control*; D000818:Animals; D000900:Anti-Bacterial Agents / Q000009:adverse effects; D000975:Antioxidants / Q000494:pharmacology / Q000627:therapeutic use*; D017209:Apoptosis / Q000187:drug effects; D001343:Autophagy / Q000187:drug effects; D001806:Blood Urea Nitrogen; D002338:Carotenoids / Q000494:pharmacology / Q000627:therapeutic use*; D002934:Cinnamates / Q000494:pharmacology / Q000627:therapeutic use*; D053630:Depsides / Q000494:pharmacology / Q000627:therapeutic use*; D004353:Drug Evaluation, Preclinical; D005839:Gentamicins / Q000009:adverse effects; D007672:Kidney Cortex / Q000187:drug effects* / Q000378:metabolism; D000077276:Lycopene; D018551:Solanum lycopersicum; D008297:Male; D008869:Microtubule-Associated Proteins / Q000378:metabolism; D052247:Nitric Oxide Synthase Type II / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects; D008517:Phytotherapy; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism; D011897:Random Allocation; D017208:Rats, Wistar; D027542:Rosmarinus; D000097123:Rosmarinic Acid
chemical_list: D000900:Anti-Bacterial Agents; D000975:Antioxidants; D002338:Carotenoids; D002934:Cinnamates; D053630:Depsides; D005839:Gentamicins; D000077276:Lycopene; D008869:Microtubule-Associated Proteins; D052247:Nitric Oxide Synthase Type II; D019253:Proto-Oncogene Proteins c-bcl-2; D000097123:Rosmarinic Acid; C000617196:Bcl2 protein, rat; C526109:Anxa3 protein, rat; C496318:Nos2 protein, rat
keywords: Bax; Bcl2; LC3/B; antioxidants; gentamicin; lycopene; nephrotoxicity; rosmarinic acid