title: Chemical constituents from the roots of Tripterygium wilfordii and their cytotoxic activity.
abstract: In our ongoing search for bioactive constituents, a new sesquiterpene polyol ester, named triptersinine U (1), together with five known triterpenes (2-6) and seven sesquiterpene pyridine alkaloids (7-13), were isolated from the roots of Tripterygium wilfordii Hook. f. Their chemical structures were elucidated using extensive spectroscopic analyses, including 1D and 2D NMR, and HRESIMS, as well as comparison with previously reported data. Cytotoxic activities of all compounds 1-13 were evaluated against six human tumor cell lines (HepG2, Hep3B, Bcap37, U251, MCF-7 and A549) using the MTT in vitro assay. The results showed that triterpenes exhibited moderate cytotoxic activities toward the tested cell lines.
authors.affiliation: a School of Traditional Chinese Materia Medica , Shenyang Pharmaceutical University , Shenyang 110016 , China.|b Key Laboratory of Structure-Based Drug Design & Discovery, Ministry of Education , Shenyang Pharmaceutical University , Shenyang 110016 , China.
authors.forename: Chang
authors.initials: C
authors.lastname: Gao
authors.affiliation: a School of Traditional Chinese Materia Medica , Shenyang Pharmaceutical University , Shenyang 110016 , China.|b Key Laboratory of Structure-Based Drug Design & Discovery, Ministry of Education , Shenyang Pharmaceutical University , Shenyang 110016 , China.
authors.forename: Li-Li
authors.initials: LL
authors.lastname: Lou
authors.affiliation: a School of Traditional Chinese Materia Medica , Shenyang Pharmaceutical University , Shenyang 110016 , China.|b Key Laboratory of Structure-Based Drug Design & Discovery, Ministry of Education , Shenyang Pharmaceutical University , Shenyang 110016 , China.
authors.forename: Di
authors.initials: D
authors.lastname: Wang
authors.affiliation: c School of Pharmaceutical Engineering , Shenyang Pharmaceutical University , Shenyang 110016 , China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: a School of Traditional Chinese Materia Medica , Shenyang Pharmaceutical University , Shenyang 110016 , China.|b Key Laboratory of Structure-Based Drug Design & Discovery, Ministry of Education , Shenyang Pharmaceutical University , Shenyang 110016 , China.
authors.forename: Xiao-Xiao
authors.initials: XX
authors.lastname: Huang
authors.affiliation: a School of Traditional Chinese Materia Medica , Shenyang Pharmaceutical University , Shenyang 110016 , China.|b Key Laboratory of Structure-Based Drug Design & Discovery, Ministry of Education , Shenyang Pharmaceutical University , Shenyang 110016 , China.
authors.forename: Shao-Jiang
authors.initials: SJ
authors.lastname: Song
mesh_terms: D000470:Alkaloids / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D004354:Drug Screening Assays, Antitumor; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D056945:Hep G2 Cells; D006801:Humans; D061986:MCF-7 Cells; D015394:Molecular Structure; D019906:Nuclear Magnetic Resonance, Biomolecular; D018517:Plant Roots / Q000737:chemistry*; D000091402:Prohibitins; D012717:Sesquiterpenes / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D029757:Tripterygium / Q000737:chemistry*; D014315:Triterpenes / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*
chemical_list: D000470:Alkaloids; D000972:Antineoplastic Agents, Phytogenic; D004365:Drugs, Chinese Herbal; C000717331:PHB2 protein, human; D000091402:Prohibitins; D012717:Sesquiterpenes; D014315:Triterpenes; C000621767:triptersinine U
keywords: Sesquiterpene polyol esters; Tripterygium wilfordii; cytotoxicity; sesquiterpene pyridine alkaloid; triterpenes