title: Production of native arbuscular mycorrhizal fungi inoculum under different environmental conditions.
abstract: In order to obtain an arbuscular mycorrhizal fungi (AMF) native inoculum from Sierra de Moa and determine the most appropriate conditions for its big scale production, four light and temperature combinations were tested in three plant species (Calophyllum antillanum, Talipariti elatum and Paspalum notatum). Growth and development parameters, as well as the mycorrhizal functioning of the seedlings were evaluated. The natural light treatment under high temperatures (L-H) was the most suitable for the growth and development of the three plant species, showing the highest total biomass values, mainly of root, and a positive root-shoot ratio balance. This treatment also promoted higher values of root mycorrhizal colonization, external mycelium and AMF spore density. A total of 38 AMF species were identified among the plants and environmental conditions tested. Archaeospora sp.1, Glomus sp.5, Glomus brohultii and G. glomerulatum were observed in all the treatments. The L-H condition can be recommended for native inoculum production, as it promotes a better expression of the AM symbiosis and an elevated production of mycorrhizal propagules.
authors.affiliation: Institute of Ecology and Systematics (CITMA), Laboratory of Mycorrhiza, Calabazar, Boyeros, La Habana, Cuba.
authors.forename: Yamir
authors.initials: Y
authors.lastname: Torres-Arias
authors.affiliation: Universidade Federal Rural do Rio de Janeiro, Instituto de Agronomia, Seropedica, RJ, Brazil.
authors.forename: Rosalba Ortega
authors.initials: RO
authors.lastname: Fors
authors.affiliation: Universidade Federal Rural do Rio de Janeiro, Instituto de Agronomia, Seropedica, RJ, Brazil.
authors.forename: Camila
authors.initials: C
authors.lastname: Nobre
authors.affiliation: Institute of Ecology and Systematics (CITMA), Laboratory of Mycorrhiza, Calabazar, Boyeros, La Habana, Cuba.
authors.forename: Eduardo Furrazola
authors.initials: EF
authors.lastname: Gómez
authors.affiliation: Universidade Federal Rural do Rio de Janeiro, Instituto de Agronomia, Seropedica, RJ, Brazil. Electronic address: berbara@ufrrj.br.
authors.forename: Ricardo Luis Louro
authors.initials: RL
authors.lastname: Berbara
mesh_terms: D015169:Colony Count, Microbial; D004777:Environment; D038821:Mycorrhizae / Q000254:growth & development; D018517:Plant Roots / Q000382:microbiology*; D036226:Seedlings / Q000254:growth & development / Q000382:microbiology; D012988:Soil Microbiology; D013172:Spores, Fungal; D013559:Symbiosis
keywords: Calophyllum antillanum; Glomeromycota; Inoculum; Paspalum notatum; Talipariti elatum