title: Development and Biosensor Applications of Novel Functional Electrodes.
abstract: Phenylboronic acid (PBA) derivatives have been used as alternatives to enzymes in the development of sugar-sensitive systems because the optical and electrochemical properties of these derivatives are significantly modulated upon sugar binding. This article reviews the voltammetric properties of sugar sensors prepared using dithiobis (4-butyrylamino-m-phenylboronic acid) (DTBA-PBA)-modified electrodes and PBA-appended layer-by-layer film-modified electrodes. In addition, the redox properties of reduced graphene oxide (rGO)-modified glassy carbon electrodes (GCEs) are discussed. The surface of a gold electrode was modified with a monolayer of DTBA-PBA to prepare sugar-sensitive electrodes. The modified electrodes exhibited attenuated cyclic voltammograms for Fe(CN)63-/4-in the presence of sugars at neutral pH as a result of their binding to DTBA-PBA on the electrode. Useful calibration curves were obtained for determining 3-300 mM D-glucose and 0.3-30 mM D-fructose. Similarly, gold electrodes coated with multilayer films composed of PBA-modified poly(allylamine hydrochloride) and carboxymethylcellulose exhibited a sugar-dependent response at neutral pH. The dynamic range of these modified electrodes was 0.1-300 mM for D-glucose and D-fructose. The surface of GCE was modified with rGO to evaluate the electrochemical response of the modified GCE to hydrogen peroxide (H2O2). The rGO-modified electrodes exhibited significantly higher responses in the redox reactions of H2O2compared with the response of an unmodified GCE.
authors.affiliation: Graduate School of Pharmaceutical Sciences, Tohoku University.
authors.forename: Shigehiro
authors.initials: S
authors.lastname: Takahashi
mesh_terms: D000499:Allylamine; D015374:Biosensing Techniques; D001897:Boronic Acids; D002138:Calibration; D002244:Carbon; D002266:Carboxymethylcellulose Sodium; D004566:Electrodes; D005632:Fructose; D005947:Glucose; D006046:Gold; D006108:Graphite; D006861:Hydrogen Peroxide / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D010084:Oxidation-Reduction; D013438:Sulfhydryl Compounds
chemical_list: D001897:Boronic Acids; D013438:Sulfhydryl Compounds; C510907:dithiobis(4-butyrylamino-m-phenylboronic acid); D005632:Fructose; D000499:Allylamine; D002244:Carbon; D006046:Gold; D006108:Graphite; D006861:Hydrogen Peroxide; D005947:Glucose; D002266:Carboxymethylcellulose Sodium; C010686:benzeneboronic acid