title: Development of a novel engineered antibody targeting Neisseria species.
abstract: OBJECTIVE
  A Neissaria bacterial pilus sugar, bacillosamine, was synthesized and, for the first time, used as a probe to screen a single-chain variable fragment (scFv).
  
  
  RESULTS
  Four Neisseria, Neisseria gonorrhoeae, Neisseria meningitidis, Neisseria sicca and Neisseria subflava, and two negative controls, Streptococcus pneumoniae and Escherichia coli, were tested through ELISA, immunostaining and gold nanoparticle immunological assay. All results indicated that the selected scFv is feasible for the specific detection of Neisseria species via the recognition of bacillosamine.
  
  
  CONCLUSIONS
  The recombinant scFv could detect Neisseria strains at 106CFU/ml.
authors.affiliation: Department of Applied Chemistry, National Chiao Tung University, Hsinchu, 30010, Taiwan.
authors.forename: Chih-Yueh
authors.initials: CY
authors.lastname: Liu
authors.affiliation: Department of Applied Chemistry, National Chiao Tung University, Hsinchu, 30010, Taiwan.
authors.forename: Chang-Ching
authors.initials: CC
authors.lastname: Weng
authors.affiliation: Department of Applied Chemistry, National Chiao Tung University, Hsinchu, 30010, Taiwan.
authors.forename: Chih-Hsiang
authors.initials: CH
authors.lastname: Lin
authors.affiliation: Institute of Molecular Biology, National Chung Hsing University, Taichung, 40227, Taiwan.
authors.forename: Chiou-Ying
authors.initials: CY
authors.lastname: Yang
authors.affiliation: Department of Applied Chemistry, National Chiao Tung University, Hsinchu, 30010, Taiwan.
authors.forename: Kwok-Kong Tony
authors.initials: KT
authors.lastname: Mong
authors.affiliation: Department of Applied Chemistry, National Chiao Tung University, Hsinchu, 30010, Taiwan. ykl@cc.nctu.edu.tw.
authors.forename: Yaw-Kuen
authors.initials: YK
authors.lastname: Li
mesh_terms: D000907:Antibodies, Bacterial / Q000276:immunology*; D000918:Antibody Specificity / Q000276:immunology; D004591:Electrophoresis, Polyacrylamide Gel; D006046:Gold / Q000737:chemistry; D053758:Nanoparticles / Q000737:chemistry; D009343:Neisseria / Q000276:immunology*; D015202:Protein Engineering / Q000379:methods*; D057127:Single-Chain Antibodies / Q000378:metabolism; D013045:Species Specificity
chemical_list: D000907:Antibodies, Bacterial; D057127:Single-Chain Antibodies; D006046:Gold
keywords: Bacillosamine; Neisseria gonorrhoeae; Neisseria meningitidis; Single-chain variable fragment