title: Immune signatures of protective spleen memory CD8 T cells.
abstract: Memory CD8 T lymphocyte populations are remarkably heterogeneous and differ in their ability to protect the host. In order to identify the whole range of qualities uniquely associated with protective memory cells we compared the gene expression signatures of two qualities of memory CD8 T cells sharing the same antigenic-specificity: protective (Influenza-induced, Flu-TM) and non-protective (peptide-induced, TIM) spleen memory CD8 T cells. Although Flu-TM and TIM express classical phenotypic memory markers and are polyfunctional, only Flu-TM protects against a lethal viral challenge. Protective memory CD8 T cells express a unique set of genes involved in migration and survival that correlate with their unique capacity to rapidly migrate within the infected lung parenchyma in response to influenza infection. We also enlighten a new set of poised genes expressed by protective cells that is strongly enriched in cytokines and chemokines such as Ccl1, Ccl9 and Gm-csf. CCL1 and GM-CSF genes are also poised in human memory CD8 T cells. These immune signatures are also induced by two other pathogens (vaccinia virus and Listeria monocytogenes). The immune signatures associated with immune protection were identified on circulating cells, i.e. those that are easily accessible for immuno-monitoring and could help predict vaccines efficacy.
authors.affiliation: CIRI, Centre International de Recherche en Infectiologie, Inserm, U1111, Université Claude Bernard Lyon 1, CNRS, UMR5308, École Normale Supérieure de Lyon, Univ Lyon, F-69007, LYON, France.
authors.forename: Lilia
authors.initials: L
authors.lastname: Brinza
authors.affiliation: CIRI, Centre International de Recherche en Infectiologie, Inserm, U1111, Université Claude Bernard Lyon 1, CNRS, UMR5308, École Normale Supérieure de Lyon, Univ Lyon, F-69007, LYON, France.
authors.forename: Sophia
authors.initials: S
authors.lastname: Djebali
authors.affiliation: CIRI, Centre International de Recherche en Infectiologie, Inserm, U1111, Université Claude Bernard Lyon 1, CNRS, UMR5308, École Normale Supérieure de Lyon, Univ Lyon, F-69007, LYON, France.
authors.forename: Martine
authors.initials: M
authors.lastname: Tomkowiak
authors.affiliation: CIRI, Centre International de Recherche en Infectiologie, Inserm, U1111, Université Claude Bernard Lyon 1, CNRS, UMR5308, École Normale Supérieure de Lyon, Univ Lyon, F-69007, LYON, France.
authors.forename: Julien
authors.initials: J
authors.lastname: Mafille
authors.affiliation: CIRI, Centre International de Recherche en Infectiologie, Inserm, U1111, Université Claude Bernard Lyon 1, CNRS, UMR5308, École Normale Supérieure de Lyon, Univ Lyon, F-69007, LYON, France.
authors.forename: Céline
authors.initials: C
authors.lastname: Loiseau
authors.affiliation: AltraBio SAS, 69007 Lyon, France.
authors.forename: Pierre-Emmanuel
authors.initials: PE
authors.lastname: Jouve
authors.affiliation: AltraBio SAS, 69007 Lyon, France.
authors.forename: Simon
authors.initials: S
authors.lastname: de Bernard
authors.affiliation: AltraBio SAS, 69007 Lyon, France.
authors.forename: Laurent
authors.initials: L
authors.lastname: Buffat
authors.affiliation: Laboratoire Virpath EA4610, Faculté de Médecine Lyon Est, Université Claude Bernard Lyon 1, Université de Lyon, France.|Laboratoire de Virologie, CNR des virus influenza, Hospices Civils de Lyon, Lyon, France.
authors.forename: Bruno
authors.initials: B
authors.lastname: Lina
authors.affiliation: Laboratoire Virpath EA4610, Faculté de Médecine Lyon Est, Université Claude Bernard Lyon 1, Université de Lyon, France.
authors.forename: Michèle
authors.initials: M
authors.lastname: Ottmann
authors.affiliation: Laboratoire Virpath EA4610, Faculté de Médecine Lyon Est, Université Claude Bernard Lyon 1, Université de Lyon, France.
authors.forename: Manuel
authors.initials: M
authors.lastname: Rosa-Calatrava
authors.affiliation: CIRI, Centre International de Recherche en Infectiologie, Inserm, U1111, Université Claude Bernard Lyon 1, CNRS, UMR5308, École Normale Supérieure de Lyon, Univ Lyon, F-69007, LYON, France.
authors.forename: Stéphane
authors.initials: S
authors.lastname: Schicklin
authors.affiliation: IRCM, Institut de Recherche en Cancérologie de Montpellier; INSERM, U896; Université Montpellier 1; CRLC Val d'Aurelle Paul Lamarque, Montpellier, France.
authors.forename: Nathalie
authors.initials: N
authors.lastname: Bonnefoy
authors.affiliation: Albert Einstein College of Medicine, Department of Microbiology and Immunology, Bronx, NY 10461, USA.
authors.forename: Grégoire
authors.initials: G
authors.lastname: Lauvau
authors.affiliation: CIRI, Centre International de Recherche en Infectiologie, Inserm, U1111, Université Claude Bernard Lyon 1, CNRS, UMR5308, École Normale Supérieure de Lyon, Univ Lyon, F-69007, LYON, France.
authors.forename: Morgan
authors.initials: M
authors.lastname: Grau
authors.affiliation: CIRI, Centre International de Recherche en Infectiologie, Inserm, U1111, Université Claude Bernard Lyon 1, CNRS, UMR5308, École Normale Supérieure de Lyon, Univ Lyon, F-69007, LYON, France.
authors.forename: Mélanie
authors.initials: M
authors.lastname: Wencker
authors.affiliation: CIRI, Centre International de Recherche en Infectiologie, Inserm, U1111, Université Claude Bernard Lyon 1, CNRS, UMR5308, École Normale Supérieure de Lyon, Univ Lyon, F-69007, LYON, France.
authors.forename: Christophe
authors.initials: C
authors.lastname: Arpin
authors.affiliation: CIRI, Centre International de Recherche en Infectiologie, Inserm, U1111, Université Claude Bernard Lyon 1, CNRS, UMR5308, École Normale Supérieure de Lyon, Univ Lyon, F-69007, LYON, France.
authors.forename: Thierry
authors.initials: T
authors.lastname: Walzer
authors.affiliation: CIRI, Centre International de Recherche en Infectiologie, Inserm, U1111, Université Claude Bernard Lyon 1, CNRS, UMR5308, École Normale Supérieure de Lyon, Univ Lyon, F-69007, LYON, France.
authors.forename: Yann
authors.initials: Y
authors.lastname: Leverrier
authors.affiliation: CIRI, Centre International de Recherche en Infectiologie, Inserm, U1111, Université Claude Bernard Lyon 1, CNRS, UMR5308, École Normale Supérieure de Lyon, Univ Lyon, F-69007, LYON, France.
authors.forename: Jacqueline
authors.initials: J
authors.lastname: Marvel
mesh_terms: D000818:Animals; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology* / Q000821:virology; D018925:Chemokines / Q000235:genetics / Q000378:metabolism; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D006706:Homeostasis; D006801:Humans; D007156:Immunologic Memory / Q000235:genetics*; D008168:Lung / Q000473:pathology; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D005810:Multigene Family; D009975:Orthomyxoviridae / Q000502:physiology; D010455:Peptides / Q000276:immunology; D010641:Phenotype; D025341:Principal Component Analysis; D013045:Species Specificity; D013154:Spleen / Q000166:cytology*
chemical_list: D018925:Chemokines; D010455:Peptides