title: Preparation and characterization of magnetic molecularly imprinted polymers for the extraction of hexamethylenetetramine in milk samples.
abstract: Magnetic molecularly imprinted polymers (M-MIPs) were synthesized as the sorbents for extracting hexamethylenetetramine (HMT) from milk samples. Molecular simulations were used to calculate the interaction energies of the template monomers. The physical properties of M-MIPs were characterized. The adsorption isotherms and kinetics were investigated. Gas chromatography coupled with tandem mass spectrometry (GC-MS/MS) was applied to determine the amount of HMT residue in milk samples. In the optimized method, a linear calibration curve was obtained using a matrix-matched standard in the range of 1.0-50.0μgL-1. The limit of detection (LOD) and limit of quantification (LOQ) was 0.3μgkg-1and 1.0μgkg-1, respectively. The relative standard deviation (RSD) of the intra-day assay ranged from 2.6% to 5.2%, while that of the inter-day assay ranged from 3.6% to 11.5%. The recovery of HMT in milk samples ranged from 88.7% to 111.4%.
authors.affiliation: State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi 214122, Jiangsu, PR China; Dali Comprehensive Inspection Centre of Quality and Technical Supervision, Dali 671000, Yunnan, PR China.
authors.forename: Xing
authors.initials: X
authors.lastname: Xu
authors.affiliation: State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi 214122, Jiangsu, PR China.
authors.forename: Emmanuel
authors.initials: E
authors.lastname: Duhoranimana
authors.affiliation: State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi 214122, Jiangsu, PR China. Electronic address: xmzhang@jiangnan.edu.cn.
authors.forename: Xiaoming
authors.initials: X
authors.lastname: Zhang
mesh_terms: D000327:Adsorption; D000818:Animals; D005591:Chemical Fractionation / Q000379:methods*; D003959:Diamines / Q000032:analysis / Q000302:isolation & purification*; D005506:Food Contamination / Q000032:analysis; D007700:Kinetics; D057230:Limit of Detection; D059346:Magnets / Q000737:chemistry*; D008892:Milk / Q000737:chemistry*; D054802:Molecular Imprinting; D011108:Polymers / Q000138:chemical synthesis* / Q000737:chemistry
chemical_list: D003959:Diamines; D011108:Polymers; C007297:1,6-diaminohexane
keywords: GC-MS/MS; Hexamethylenetetramine; Magnetic molecularly imprinted polymers; Magnetic solid phase extraction; Milk