title: Expression Profiling of Antibiotic-Resistant Bacteria Obtained by Laboratory Evolution.
abstract: To elucidate the mechanisms of antibiotic resistance, integrating phenotypic and genotypic features in resistant strains is important. Here, we describe the expression profiling of antibiotic-resistant Escherichia coli strains obtained by laboratory evolution, and a method for extracting a small number of genes whose expression changes can contribute to the acquisition of resistance.
authors.affiliation: Quantitative Biology Center, RIKEN, 6-2-3 Furuedai, Suita, Osaka, 565-0874, Japan.
authors.forename: Shingo
authors.initials: S
authors.lastname: Suzuki
authors.affiliation: Quantitative Biology Center, RIKEN, 6-2-3 Furuedai, Suita, Osaka, 565-0874, Japan.
authors.forename: Takaaki
authors.initials: T
authors.lastname: Horinouchi
authors.affiliation: Quantitative Biology Center, RIKEN, 6-2-3 Furuedai, Suita, Osaka, 565-0874, Japan. chikara.furusawa@riken.jp.
authors.forename: Chikara
authors.initials: C
authors.lastname: Furusawa
mesh_terms: D001419:Bacteria / Q000235:genetics*; D019020:Directed Molecular Evolution; D004352:Drug Resistance, Microbial / Q000235:genetics*; D020869:Gene Expression Profiling / Q000379:methods*; D015964:Gene Expression Regulation, Bacterial; D020411:Oligonucleotide Array Sequence Analysis; D012329:RNA, Bacterial / Q000235:genetics / Q000378:metabolism
chemical_list: D012329:RNA, Bacterial
keywords: Antibiotic resistance; Escherichia coli; Laboratory evolution; Transcriptome analysis