title: The expression research of miR-210 in the elderly patients with COPD combined with ischemic stroke.
abstract: We conduct this study to investigate the expression level of miR-210 in elderly patients with COPD combined with ischemic stroke and analyzed its application value as a sensitive diagnostic indicator.
  
  
  
  50 cases of elderly patients with COPD combined with ischemic stroke were selected as group A, 50 cases of elderly patients with COPD were selected as group B, 50 cases of elderly patients with ischemic stroke were selected as group C, and 50 cases of healthy volunteers as group D. Real-time PCR assay for quantification was used to detect the expression level of miR-210 in peripheral blood and receiver operating curve (ROC) was used to analyze the diagnostic sensitivity and accuracy.
  
  
  
  MiR-210 level of group A was the lowest, followed by group B and group C; group D had the highest levels. The difference was statistically significant (p<0.05). MiR-210 levels decreased with an increasing decline degree of lung function and the difference was statistically significant (p<0.05). After miR-210 diagnosis, COPD sensitivity was 85.6%, the specificity was 72.6%, accuracy (AUC) was 0.821, and 95% CI 0.632-0.924.
  
  
  
  The down-regulation of MiR-210 expression in the COPD combined with ischemic stroke can be regarded as a sensitive index in diagnosis of COPD and ischemic stroke.
authors.affiliation: Department of Respiratory Medicine, Weifang People's Hospital, Weifang, Shandong, China. hua_defeng1@163.com.
authors.forename: L-Q
authors.initials: LQ
authors.lastname: Wang
authors.forename: C-L
authors.initials: CL
authors.lastname: Wang
authors.forename: L-N
authors.initials: LN
authors.lastname: Xu
authors.forename: D-F
authors.initials: DF
authors.lastname: Hua
mesh_terms: D000368:Aged; D015415:Biomarkers / Q000097:blood*; D015536:Down-Regulation; D006801:Humans; D035683:MicroRNAs / Q000378:metabolism*; D029424:Pulmonary Disease, Chronic Obstructive / Q000235:genetics*; D012372:ROC Curve; D020521:Stroke / Q000235:genetics*
chemical_list: D015415:Biomarkers; C543800:MIRN210 microRNA, human; D035683:MicroRNAs