title: Comparison of antibacterial effects among three foams used with negative pressure wound therapy in an ex vivo equine perfused wound model.
abstract: OBJECTIVE To compare antibacterial effects among 3 types of foam used with negative-pressure wound therapy (NPWT) in an ex vivo equine perfused wound model. SAMPLES Abdominal musculocutaneous flaps from 6 equine cadavers. PROCEDURES Each musculocutaneous flap was continuously perfused with saline (0.9% NaCl) solution. Four 5-cm circular wounds were created in each flap and contaminated with 106CFUs of both Pseudomonas aeruginosa and methicillin-resistant Staphylococcus aureus (MRSA). After a 1-hour incubation period, 1 of 4 treatments (NPWT with silver-impregnated polyurethane foam [NPWT-AgPU], polyurethane foam [NPWT-PU], or polyvinyl alcohol foam [NPWT-PVA] or a nonadherent dressing containing polyhexamethylene biguanide without NPWT [control]) was randomly applied to each wound. An 8-mm punch biopsy specimen was obtained from each wound immediately before and at 6, 12, 18, and 24 hours after treatment application to determine the bacterial load for both P aeruginosa and MRSA. RESULTS The bacterial load of P aeruginosa for the NPWT-PVA treatment was significantly lower than that for the other 3 treatments at each sampling time after application, whereas the bacterial load for the NPWT-AgPU treatment was significantly lower than that for the NPWT-PU and control treatments at 12 hours after application. The bacterial load of MRSA for the NPWT-PVA treatment was significantly lower than that for the other 3 treatments at each sampling time after application. CONCLUSIONS AND CLINICAL RELEVANCE Results indicated that wounds treated with NPWT-PVA had the greatest decrease in bacterial load; however, the effect of that treatment on wound healing needs to be assessed in vivo.
authors.forename: Lore L
authors.initials: LL
authors.lastname: Van Hecke
authors.forename: Maarten
authors.initials: M
authors.lastname: Haspeslagh
authors.forename: Katleen
authors.initials: K
authors.lastname: Hermans
authors.forename: Ann M
authors.initials: AM
authors.lastname: Martens
mesh_terms: D000005:Abdomen; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D058491:Bacterial Load; D002102:Cadaver; D006736:Horses; D055624:Methicillin-Resistant Staphylococcus aureus / Q000187:drug effects; D023421:Models, Animal; D054843:Negative-Pressure Wound Therapy / Q000662:veterinary*; D011140:Polyurethanes / Q000494:pharmacology*; D011142:Polyvinyl Alcohol / Q000494:pharmacology; D011550:Pseudomonas aeruginosa / Q000187:drug effects; D012834:Silver / Q000494:pharmacology; D012867:Skin / Q000187:drug effects* / Q000382:microbiology / Q000473:pathology; D013524:Surgical Flaps / Q000662:veterinary; D014945:Wound Healing; D014946:Wound Infection / Q000382:microbiology / Q000473:pathology / Q000517:prevention & control*
chemical_list: D000900:Anti-Bacterial Agents; D011140:Polyurethanes; D012834:Silver; D011142:Polyvinyl Alcohol; C028279:polyurethane foam