title: Purification and characterization of an antibacterial and anti-inflammatory polypeptide from Arca subcrenata.
abstract: A polypeptide coded as PGC was isolated from Arca subcrenata muscle using ion exchange, Sephadex G-50 gel chromatography and RP-HPLC. PGC was identified to be a homogeneous compound by Native-PAGE and the purity was more than 98.9% measured by HPLC. The isoelectric point of PGC was determined to be 9.76 by IEF-PAGE. The molecular weight was determined to be 15,973.0Da by ESI-MS/MS. The conformational structure of PGC was characterized by UV-vis, FT-IR and CD spectroscopy. N terminal amino acid sequence of PGC was shown as PSVYDAAAQLTADVKKDLRDSWKVIGGDKKGNGVA by Edman degradation. The results demonstrated that there is a high degree of homology between PGC and the subunit from hemoglobin, and proposed that PGC is the depolymerized polypeptide of Hemoglobin I (HbI) from A. subcrenata. The evaluation of biological activities showed that the diameters of the inhibitory ring of PGC on Escherichia coli and Staphylococcus aureus were 14.5±0.44mm and 16.5±1.15mm, respectively. The IC50of inhibition rate for PGC on NO production was 9.60±0.71μg/mL. Therefore, PGC might be developed as one of potential antibacterial and anti-inflammatory agents.
authors.affiliation: Biotechnological Institute of Chinese Materia Medica, Jinan University, 601 Huangpu Avenue West, Guangzhou 510632, China.
authors.forename: Yuyan
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Biotechnological Institute of Chinese Materia Medica, Jinan University, 601 Huangpu Avenue West, Guangzhou 510632, China; Department of Pharmacology, College of Pharmacy, Jinan University, 601 Huangpu Avenue West, Guangzhou 510632, China, China.
authors.forename: Chunlei
authors.initials: C
authors.lastname: Li
authors.affiliation: Department of Natural Medicinal Chemistry, College of Pharmacy, Jinan University, Guangzhou 510632, China.
authors.forename: Jianhua
authors.initials: J
authors.lastname: Zhu
authors.affiliation: Department of Pharmacology, College of Pharmacy, Jinan University, 601 Huangpu Avenue West, Guangzhou 510632, China, China.
authors.forename: Wangshi
authors.initials: W
authors.lastname: Xie
authors.affiliation: Department of Pharmacology, College of Pharmacy, Jinan University, 601 Huangpu Avenue West, Guangzhou 510632, China, China.
authors.forename: Xianjing
authors.initials: X
authors.lastname: Hu
authors.affiliation: Department of Pharmacology, College of Pharmacy, Jinan University, 601 Huangpu Avenue West, Guangzhou 510632, China, China. Electronic address: tsly@jnu.edu.cn.
authors.forename: Liyan
authors.initials: L
authors.lastname: Song
authors.affiliation: Biotechnological Institute of Chinese Materia Medica, Jinan University, 601 Huangpu Avenue West, Guangzhou 510632, China.
authors.forename: Jiachen
authors.initials: J
authors.lastname: Zi
authors.affiliation: Biotechnological Institute of Chinese Materia Medica, Jinan University, 601 Huangpu Avenue West, Guangzhou 510632, China; Department of Natural Medicinal Chemistry, College of Pharmacy, Jinan University, Guangzhou 510632, China. Electronic address: tyrm@jnu.edu.cn.
authors.forename: Rongmin
authors.initials: R
authors.lastname: Yu
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D000893:Anti-Inflammatory Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D049893:Arcidae / Q000737:chemistry*; D001419:Bacteria / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D051379:Mice; D009569:Nitric Oxide / Q000096:biosynthesis; D010455:Peptides / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D000067996:RAW 264.7 Cells
chemical_list: D000900:Anti-Bacterial Agents; D000893:Anti-Inflammatory Agents; D010455:Peptides; D009569:Nitric Oxide
keywords: Anti-inflammatory activity; Antibacterial activity; Arca subcrenata polypeptide