title: Matrix Solid-Phase Dispersion Coupled with High-Performance Liquid Chromatography Diode Array Detection for Simultaneous Determination of Four Lipophilic Constituents from Salvia miltiorrhiza Bunge.
abstract: A simple, rapid and efficient method based on matrix solid-phase dispersion coupled with high-performance liquid chromatography was developed for determination of lipophilic constituents, including dihydrotanshinone, tanshinone I, cryptotanshinone and tanshinone II A in Salvia miltiorrhiza Bunge Box-Behnken design was employed for optimization of the extraction conditions of matrix solid-phase dispersion, including mass ratio of dispersant to sample, volume of elution solvent, and amount of cleanup reagent. The optimal experimental results were obtained using 0.27 g of acid alumina as dispersant, 13 mL of acetonitrile as elution solvent and 0.36 g of acid alumina as cleanup reagent. The target analytes was determined by high-performance liquid chromatography. The recoveries of tanshinones obtained by analyzing the spiked samples were from 83.81% to 93.74% and relative standard deviations from 2.87% to 6.83%. Matrix solid-phase dispersion integrated the extraction and cleanup into a single step, which provides the advantages of being simple, fast and convenient. Compared with other conventional methods, the present method consumed less time and less organic solvent. The results demonstrate that this method has potential for the determination of active constituents and the quality control of traditional Chinese medicine.
authors.affiliation: College of Chemistry and Life Science, Changchun University of Technology, Changchun, 130012, P.R. China.|College of Chemistry, Jilin University, Changchun 130012, P.R. China.
authors.forename: Zhibing
authors.initials: Z
authors.lastname: Wang
authors.affiliation: College of Chemistry and Life Science, Changchun University of Technology, Changchun, 130012, P.R. China.
authors.forename: Siyu
authors.initials: S
authors.lastname: Ma
authors.affiliation: College of Chemistry and Life Science, Changchun University of Technology, Changchun, 130012, P.R. China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: College of Chemistry and Life Science, Changchun University of Technology, Changchun, 130012, P.R. China.
authors.forename: Shuang
authors.initials: S
authors.lastname: He
authors.affiliation: College of Chemistry and Life Science, Changchun University of Technology, Changchun, 130012, P.R. China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Li
authors.affiliation: College of Chemistry and Life Science, Changchun University of Technology, Changchun, 130012, P.R. China.
authors.forename: Jianxue
authors.initials: J
authors.lastname: Hu
authors.affiliation: College of Chemistry, Jilin University, Changchun 130012, P.R. China.
authors.forename: Hanqi
authors.initials: H
authors.lastname: Zhang
mesh_terms: D045784:Abietanes / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D057230:Limit of Detection; D016014:Linear Models; D015233:Models, Statistical; D010616:Phenanthrenes / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification; D010936:Plant Extracts / Q000737:chemistry*; D015203:Reproducibility of Results; D027561:Salvia miltiorrhiza / Q000737:chemistry*; D052616:Solid Phase Extraction / Q000379:methods*
chemical_list: D045784:Abietanes; D010616:Phenanthrenes; D010936:Plant Extracts; C021751:tanshinone