title: In vivo vizualisation of mono-ADP-ribosylation by dPARP16 upon amino-acid starvation.
abstract: PARP catalysed ADP-ribosylation is a post-translational modification involved in several physiological and pathological processes, including cellular stress. In order to visualise both Poly-, and Mono-, ADP-ribosylation in vivo, we engineered specific fluorescent probes. Using them, we show that amino-acid starvation triggers an unprecedented display of mono-ADP-ribosylation that governs the formation of Sec body, a recently identified stress assembly that forms in Drosophila cells. We show that dPARP16 catalytic activity is necessary and sufficient for both amino-acid starvation induced mono-ADP-ribosylation and subsequent Sec body formation and cell survival. Importantly, dPARP16 catalyses the modification of Sec16, a key Sec body component, and we show that it is a critical event for the formation of this stress assembly. Taken together our findings establish a novel example for the role of mono-ADP-ribosylation in the formation of stress assemblies, and link this modification to a metabolic stress.
authors.affiliation: Hubrecht Institute-KNAW, Utrecht, The Netherlands.|University Medical Center Utrecht, Utrecht, Netherlands.
authors.forename: Angelica
authors.initials: A
authors.lastname: Aguilera-Gomez
authors.affiliation: Hubrecht Institute-KNAW, Utrecht, The Netherlands.|University Medical Center Utrecht, Utrecht, Netherlands.
authors.forename: Marinke M
authors.initials: MM
authors.lastname: van Oorschot
authors.affiliation: Department of Cell Biology, University Medical Center Utrecht, Utrecht, The Netherlands.
authors.forename: Tineke
authors.initials: T
authors.lastname: Veenendaal
authors.affiliation: Hubrecht Institute-KNAW, Utrecht, The Netherlands.|University Medical Center Utrecht, Utrecht, Netherlands.|Department of Cell Biology, University Medical Center Utrecht, Utrecht, The Netherlands.
authors.forename: Catherine
authors.identifier: 0000-0002-3663-9717
authors.initials: C
authors.lastname: Rabouille
mesh_terms: D000074744:ADP-Ribosylation; D000246:Adenosine Diphosphate Ribose / Q000378:metabolism*; D000596:Amino Acids / Q000378:metabolism*; D000818:Animals; D002460:Cell Line; D002470:Cell Survival; D004330:Drosophila; D029721:Drosophila Proteins / Q000378:metabolism*; D005456:Fluorescent Dyes / Q000032:analysis; D061848:Optical Imaging / Q000379:methods; D011065:Poly(ADP-ribose) Polymerases / Q000378:metabolism*; D013217:Starvation
chemical_list: D000596:Amino Acids; D029721:Drosophila Proteins; D005456:Fluorescent Dyes; D000246:Adenosine Diphosphate Ribose; C000625488:PARP16 protein, Drosophila; D011065:Poly(ADP-ribose) Polymerases
keywords: D. melanogaster; PARP16; Sec16; amino-acid starvation; cell biology; fluorescence probe; mono-ADP-ribosylation; stress assemblies