title: Using Monoclonal Antibody to Determine Lead Ions with a Localized Surface Plasmon Resonance Fiber-optic Biosensor.
abstract: A novel reflection-based localized surface plasmon resonance (LSPR) fiber-optic probe has been developed to determine the heavy metal lead ion concentration. Monoclonal antibody as the detecting probe containing massive amino groups to capture Pb(II)-chelate complexes was immobilized onto gold nanoparticle-modified optical fiber (NMAuOF). The optimal immobilizing conditions of monoclonal antibody on to the NMAuOF are 189 μg/mL in pH7.4 PBS for 2 h at 25°C. The absorbability of the functionalized NMAuOF sensor increases to 12.2 % upon changing the Pb(II)-EDTA level from 10 to 100 ppb with a detection limit of 0.27 ppb. The sensor retains 92.7 % of its original activity and gives reproducible results after storage in 5% D-( )-Trehalose dehydrate solution at 4°C for 35 days. In conclusion, the monoclonal antibody-functionalized NMAuOF sensor shows a promising result for determining the concentration of Pb(II) with high sensitivity.
authors.affiliation: Department of Chemical Engineering, National Chung-Cheng University, Chia-Yi 621, Taiwan. chmtjl@ccu.edu.tw.
authors.forename: Tsao-Jen
authors.initials: TJ
authors.lastname: Lin
authors.affiliation: Department of Chemical Engineering, National Chung-Cheng University, Chia-Yi 621, Taiwan.
authors.forename: Mon-Fu
authors.initials: MF
authors.lastname: Chung
keywords: biosensor; gold nanoparticle; lead-chelate complex; lead-chelate complex. antibody; localized surface plasmon resonance; monoclonal antibody