title: Quantitative proteomic analysis reveals that proteins required for fatty acid metabolism may serve as diagnostic markers for gastric cancer.
abstract: BACKGROUND
  Gastric cancer is one of the leading causes of cancer-related deaths worldwide. The sensitivities and specificities of current biomarkers for gastric cancer are insufficient for clinical detection, and new diagnostic tests are therefore urgently required.
  
  
  METHODS
  A discovery set of gastric cancer and adjacent normal tissues were analyzed for differentially expressed proteins by labeling of peptide digests with isobaric tag for relative and absolute quantitation (iTRAQ) reagents followed by liquid chromatography-electrospray ionization-tandem mass spectrometry. A validation set of 70 pairs of gastric cancer and adjacent normal tissues were examined to confirm the expression levels of the potential biomarkers identified by iTRAQ labeling.
  
  
  RESULTS
  We detected 431 proteins associated with 16 KEGG pathways that were differentially expressed in gastric cancer tissues, of which 224 were upregulated and 207 were downregulated in gastric cancer tissues. Coexpression of fatty acid binding protein (FABP1) and fatty acid synthase (FASN) in gastric cancer tissues (61.4% sensitivity and 77.1% specificity) was strongly associated with lymph node metastasis and Tumor, Node, Metastasis stage I/II.
  
  
  CONCLUSIONS
  Quantitative proteomic analysis of gastric cancer tissues revealed that coexpression of FABP1 and FASN may serve as a biomarker for detection of early gastric cancer.
authors.affiliation: Department of Biochemistry, School of Preclinical Medicine, North Sichuan Medical College, Nanchong, Sichuan Province 637100, PR China.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Jiang
authors.affiliation: College of Integrated Chinese and Western Medicine, Southwest Medical University, Luzhou, Sichuan Province 646000, PR China.
authors.forename: Hongchun
authors.initials: H
authors.lastname: Shen
authors.affiliation: Department of Pathology, Affiliated Traditional Medicine Hospital, Southwest Medical University, Luzhou, Sichuan 646000, PR China.
authors.forename: Bo
authors.initials: B
authors.lastname: Tang
authors.affiliation: Research Center of Combine Traditional Chinese and Western Medicine, Affiliated Traditional Medicine Hospital, Southwest Medical University, Luzhou, Sichuan 646000, PR China.
authors.forename: Qin
authors.initials: Q
authors.lastname: Yu
authors.affiliation: Research Center of Combine Traditional Chinese and Western Medicine, Affiliated Traditional Medicine Hospital, Southwest Medical University, Luzhou, Sichuan 646000, PR China.
authors.forename: Xingli
authors.initials: X
authors.lastname: Ji
authors.affiliation: Research Center of Combine Traditional Chinese and Western Medicine, Affiliated Traditional Medicine Hospital, Southwest Medical University, Luzhou, Sichuan 646000, PR China. Electronic address: 1999wangli@163.com.
authors.forename: Li
authors.initials: L
authors.lastname: Wang
mesh_terms: D014408:Biomarkers, Tumor / Q000378:metabolism*; D055088:Early Detection of Cancer; D054890:Fatty Acid Synthase, Type I / Q000378:metabolism; D050556:Fatty Acid-Binding Proteins / Q000378:metabolism; D005227:Fatty Acids / Q000378:metabolism*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D040901:Proteomics; D013274:Stomach Neoplasms / Q000175:diagnosis* / Q000378:metabolism* / Q000473:pathology
chemical_list: D014408:Biomarkers, Tumor; C493319:FABP1 protein, human; D050556:Fatty Acid-Binding Proteins; D005227:Fatty Acids; C518729:FASN protein, human; D054890:Fatty Acid Synthase, Type I
keywords: FABP1; FASN; Gastric cancer; Lipid metabolism; iTRAQ