title: Inhibition of protein glycation, antioxidant and antiproliferative activities of Carpobrotus edulis extracts.
abstract: Carpobrotus edulis is an important South African medicinal plants used as a food and therapeutic agent in traditional medicine. The aim of this study was to determine the phytochemical content, antioxidant, antiglycation and cytotoxic effect against Human Colon Cancer Cell Line (HCT-116) of aqueous and ethanol-water (1:1v/v) extracts of Carpobrotus edulis.The content of total phenolics and flavonoids in aqueous and ethanol-water extract were 151.99μg and 66.35μg gallic acid equivalents/mg of dry extract, and 38.84μg and 21.96μg quercetin/mg of dry extract, respectively. Furthermore, phenolic compositions analysis indicated the presence of seven majority compounds including sinapic acid, ferulic acid, luteolin7-o-glucoside, hyperoside, isoquercitrin, ellagic acid and isorhamnetin 3-O-rutinoside. The ethanol-water extract (100-1000μg/mL) showed better antioxidant activity than aqueous extract. Furthermore, Carpobrotus edulis extracts, especially ethanol-water extract significantly inhibited the formation of fluorescent advanced glycation end products, prevented oxidation-induced protein damage and exhibited a cytotoxic effect against HCT116 cells, with a significant decrease in cell viability after 24h of incubation. The results obtained suggest that the Carpobrotus edulis extracts could be used as an easily accessible source of natural antioxidants and as potential phytochemicals against protein glycation and colon cancer.
authors.affiliation: Department of Biochemistry, Faculty of Medicine of Sousse, University of Sousse, 4002, Tunisie. Electronic address: jawhar_hafsa@yahoo.fr.
authors.forename: Jawhar
authors.initials: J
authors.lastname: Hafsa
authors.affiliation: Laboratory of Medicinal and Aromatic Plants, Biotechnology Centre of BorjCedria (CBBC), BP 901, 2050 Hammam-Lif, Tunisie; Laboratory of interfaces and advanced materials, Faculty of Sciences of Monastir, University of Monastir, 5000, Tunisie.
authors.forename: Khaoula Mkadmini
authors.initials: KM
authors.lastname: Hammi
authors.affiliation: Laboratory of Biochemistry, 'Nutrition, Functional Food and Vascular Health' Faculty of Medicine of Monastir, University of Monastir, 5000, Tunisie.
authors.forename: Med Raâfet Ben
authors.initials: MRB
authors.lastname: Khedher
authors.affiliation: Department of Biochemistry, Faculty of Medicine of Sousse, University of Sousse, 4002, Tunisie.
authors.forename: Med Ali
authors.initials: MA
authors.lastname: Smach
authors.affiliation: Department of Biochemistry, Faculty of Medicine of Sousse, University of Sousse, 4002, Tunisie.
authors.forename: Bassem
authors.initials: B
authors.lastname: Charfeddine
authors.affiliation: Department of Biochemistry, Faculty of Medicine of Sousse, University of Sousse, 4002, Tunisie.
authors.forename: Khalifa
authors.initials: K
authors.lastname: Limem
authors.affiliation: Laboratory of interfaces and advanced materials, Faculty of Sciences of Monastir, University of Monastir, 5000, Tunisie. Electronic address: hatemmajdoub.fsm@gmail.com.
authors.forename: Hatem
authors.initials: H
authors.lastname: Majdoub
mesh_terms: D000975:Antioxidants / Q000302:isolation & purification / Q000494:pharmacology*; D049109:Cell Proliferation / Q000187:drug effects* / Q000502:physiology; D002470:Cell Survival / Q000187:drug effects / Q000502:physiology; D004305:Dose-Response Relationship, Drug; D017127:Glycation End Products, Advanced / Q000037:antagonists & inhibitors* / Q000502:physiology; D045325:HCT116 Cells; D006801:Humans; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology*; D010946:Plants, Medicinal
chemical_list: D000975:Antioxidants; D017127:Glycation End Products, Advanced; D010936:Plant Extracts
keywords: Antiglycation; Antioxidant; Antiproliferative; Carpobrotus edulis; HCT-116; Phenolic compounds