title: Stanniocalcin 2 improved osteoblast differentiation via phosphorylation of ERK.
abstract: Mammalian stanniocalcin 2 (STC2) is a glycoprotein hormone with multiple functions. The present study determined the importance of STC2 in osteoblast differentiation. It was revealed that the expression of STC2 was increased during the differentiation of MC3T3-E1 cells to osteoblasts and that knockdown of STC2 reduced osteoblast differentiation and mineralization, whereas STC2 overexpression increased differentiation and mineralization. Knockdown of STC2 downregulated the expression of osteoblast-associated genes, including runt‑related transcription factor 2, collagen type I α 1 chain, osterix and osteocalcin. Overexpression of STC2 upregulated the expression of these osteoblastic genes. In addition, overexpression of STC2 enhanced the phosphorylation of extracellular signal‑regulated kinase 1/2 (ERK1/2), whereas inhibition of ERK phosphorylation reduced osteoblast differentiation of MC3T3‑E1 cells overexpressing STC2. These findings indicated that STC2 may promote osteoblast differentiation and mineralization by regulating ERK activation.
authors.affiliation: Central Laboratory, The Fifth People's Hospital of Shanghai, Fudan University, Shanghai 200240, P.R. China.
authors.forename: Juan
authors.initials: J
authors.lastname: Zhou
authors.affiliation: Central Laboratory, The Fifth People's Hospital of Shanghai, Fudan University, Shanghai 200240, P.R. China.
authors.forename: Yinghua
authors.initials: Y
authors.lastname: Li
authors.affiliation: Central Laboratory, The Fifth People's Hospital of Shanghai, Fudan University, Shanghai 200240, P.R. China.
authors.forename: Lina
authors.initials: L
authors.lastname: Yang
authors.affiliation: Central Laboratory, The Fifth People's Hospital of Shanghai, Fudan University, Shanghai 200240, P.R. China.
authors.forename: Yougen
authors.initials: Y
authors.lastname: Wu
authors.affiliation: Central Laboratory, The Fifth People's Hospital of Shanghai, Fudan University, Shanghai 200240, P.R. China.
authors.forename: Yunjiao
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: Central Laboratory, The Fifth People's Hospital of Shanghai, Fudan University, Shanghai 200240, P.R. China.
authors.forename: Yunqing
authors.initials: Y
authors.lastname: Cui
authors.affiliation: Central Laboratory, The Fifth People's Hospital of Shanghai, Fudan University, Shanghai 200240, P.R. China.
authors.forename: Gong
authors.initials: G
authors.lastname: Yang
authors.affiliation: Central Laboratory, The Fifth People's Hospital of Shanghai, Fudan University, Shanghai 200240, P.R. China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Hong
mesh_terms: D000818:Animals; D002113:Calcification, Physiologic / Q000235:genetics; D002454:Cell Differentiation / Q000235:genetics; D002460:Cell Line; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism*; D015870:Gene Expression; D055785:Gene Knockdown Techniques; D006023:Glycoproteins / Q000235:genetics / Q000378:metabolism*; D036341:Intercellular Signaling Peptides and Proteins; D047908:Intracellular Signaling Peptides and Proteins; D051379:Mice; D009928:Organ Specificity / Q000235:genetics; D010006:Osteoblasts / Q000166:cytology* / Q000378:metabolism*; D010766:Phosphorylation
chemical_list: D006023:Glycoproteins; D036341:Intercellular Signaling Peptides and Proteins; D047908:Intracellular Signaling Peptides and Proteins; C485438:Stc2 protein, mouse; D048049:Extracellular Signal-Regulated MAP Kinases