title: Accelerated colorimetric immunosensing using surface-modified porous monoliths and gold nanoparticles.
abstract: A rapid and sensitive immunoassay platform integrating polymerized monoliths and gold nanoparticles (AuNPs) has been developed. The porous monoliths are photopolymerizedin situwithin a silica capillary and serve as solid support for high-mass transport and high-density capture antibody immobilization to create a shorter diffusion length for antibody-antigen interactions, resulting in a rapid assay and low reagent consumption. AuNPs are modified with detection antibodies and are utilized as signals for colorimetric immunoassays without the need for enzyme, substrate and sophisticated equipment for quantitative measurements. This platform has been verified by performing a human IgG sandwich immunoassay with a detection limit of 0.1 ng ml-1. In addition, a single assay can be completed in 1 h, which is more efficient than traditional immunoassays that require several hours to complete.
authors.affiliation: Graduate Institute of Biomedical Engineering, National Chung Hsing University, Taichung 402, Taiwan.
authors.forename: Shao-Hsuan
authors.initials: SH
authors.lastname: Chuag
authors.affiliation: Graduate Institute of Biomedical Engineering, National Chung Hsing University, Taichung 402, Taiwan.
authors.forename: Guan-Hua
authors.initials: GH
authors.lastname: Chen
authors.affiliation: Graduate Institute of Biomedical Engineering, National Chung Hsing University, Taichung 402, Taiwan.
authors.forename: Hsin-Hao
authors.initials: HH
authors.lastname: Chou
authors.affiliation: Graduate Institute of Biomedical Engineering, National Chung Hsing University, Taichung 402, Taiwan.
authors.forename: Shu-Wei
authors.initials: SW
authors.lastname: Shen
authors.affiliation: Graduate Institute of Biomedical Engineering, National Chung Hsing University, Taichung 402, Taiwan.
authors.forename: Chien-Fu
authors.initials: CF
authors.lastname: Chen
keywords: colorimetric assay; gold nanoparticles; immunoassay; porous monoliths