title: Varietal Dependence of GLVs Accumulation and LOX-HPL Pathway Gene Expression in Four Vitis vinifera Wine Grapes.
abstract: Variety is one of the major factors influencing grape and wine aromatic characteristics. Green leaf volatiles (GLVs), derived from lipoxygenase-hydroperoxides lyase (LOX-HPL) pathway, are important components for the aromatic quality of grapes and wines. However, the varietal difference regarding GLVs accumulation and related gene expression are poorly studied. This work exhibited that the accumulation of various GLVs and the expression of LOX-HPL pathway genes in fourVitis viniferawine grape cultivars: Syrah, Muscat Tchervine, Gewürztraminer and Chardonnay. The results showed a variety dependence of GLVs profile. Muscat Tchervine harvested grapes contained less C6 aldehydes and the most abundant esters, which corresponded to very lowVvLOXAandVvHPL1expression abundance as well as highVvAATtranscript in this variety. High expression level of bothVvLOXAandVvHPL1paralleled with higher level of C6 aldehydes together with higher alcohols in Syrah grape. Gewürztraminer and Chardonnay grapes had high aldehydes and alcohols as well as low esters, which were resulted from their higher expression level ofVvLOXAorVvHPL1and lowerVvAAT. From these above corresponding relations, it is concluded thatVvLOXA,VvHPL1andVvAATin the LOX-HPL pathway are targets for altering GLVs composition in the grape varieties.
authors.affiliation: Center for Viticulture & Enology, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China. qxhanyi@163.com.
authors.forename: Xu
authors.initials: X
authors.lastname: Qian
authors.affiliation: Center for Viticulture & Enology, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China. xuxiaoqing@outlook.com.
authors.forename: Xiao-Qing
authors.initials: XQ
authors.lastname: Xu
authors.affiliation: Center for Viticulture & Enology, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China. yukeji@cau.edu.cn.
authors.forename: Ke-Ji
authors.initials: KJ
authors.lastname: Yu
authors.affiliation: Department of Food Science and Engineering, College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, China. Zhubaoqing@gmail.com.
authors.forename: Bao-Qing
authors.initials: BQ
authors.lastname: Zhu
authors.affiliation: Center for Viticulture & Enology, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China. clanyibin@gmail.com.
authors.forename: Yi-Bin
authors.initials: YB
authors.lastname: Lan
authors.affiliation: Center for Viticulture & Enology, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China. chqduan@cau.edu.cn.
authors.forename: Chang-Qing
authors.initials: CQ
authors.lastname: Duan
authors.affiliation: Center for Viticulture & Enology, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China. panqh@cau.edu.cn.
authors.forename: Qiu-Hong
authors.initials: QH
authors.lastname: Pan
mesh_terms: D000438:Alcohols / Q000378:metabolism; D000446:Aldehyde-Lyases / Q000235:genetics* / Q000378:metabolism; D000447:Aldehydes / Q000378:metabolism; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics* / Q000378:metabolism; D016002:Discriminant Analysis; D005421:Flavoring Agents / Q000378:metabolism; D005638:Fruit / Q000235:genetics* / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D014644:Genetic Variation; D008084:Lipoxygenase / Q000235:genetics* / Q000378:metabolism; D018515:Plant Leaves / Q000235:genetics* / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction; D013045:Species Specificity; D027843:Vitis / Q000145:classification / Q000235:genetics* / Q000378:metabolism; D014920:Wine / Q000032:analysis
chemical_list: D000438:Alcohols; D000447:Aldehydes; D005421:Flavoring Agents; D010940:Plant Proteins; D012333:RNA, Messenger; D003577:Cytochrome P-450 Enzyme System; D008084:Lipoxygenase; D000446:Aldehyde-Lyases; C054540:hydroperoxide lyase
keywords: grape variety; green leaf volatiles; lipoxygenase-hydroperoxide lyase pathway