title: HUWE1 plays important role in mouse preimplantation embryo development and the dysregulation is associated with poor embryo development in humans.
abstract: HUWE1 is a HECT domain containing ubiquitin ligase implicated in neurogenesis, spermatogenesis and cancer development. The purpose of the current study is to investigate the role of HUWE1 in early embryo development. Here we demonstrate that Huwe1 is expressed in both nucleus and cytoplasm of preimplantation mouse embryos as well as gametes. Hypoxia (5% O2) treatment could significantly increase Huwe1 expression during mouse embryo development process. HUWE1 knockdown inhibited normal embryonic development and reduced blastocyst formation, and increased apoptotic cell numbers were observed in the embryos of HUWE1 knockdown group. Human embryo staining result showed that reduced HUWE1 staining was observed in the poor-quality embryos. Furthermore, Western blot result showed that significantly reduced expression of HUWE1 was observed in the villi of miscarriage embryos compared with the normal control, indicating that reduced expression of HUWE1 is related to poor embryo development. Oxidative reagent, H2O2inhibited HUWE1 expression in human sperm, indicating that HUWE1 expression in sperm is regulated by oxidative stress. In conclusion, these results suggest that HUWE1 protein could contribute to preimplantation embryo development and dysregulated expression of HUWE1 could be related to poor embryo development and miscarriage in IVF clinic.
authors.affiliation: Department of Obstetric and Gynecologic diseases, West China Second University Hospital, Sichuan University, Chengdu 610041, P.R. China.|SCU-CUHK Joint Laboratory of Reproductive Medicine, Key Laboratory of Birth Defects and Related Diseases of Women and Children, Ministry of Education, (Sichuan University), West China Second University Hospital, Sichuan University, Chengdu 610041, P.R. China.
authors.forename: L J
authors.initials: LJ
authors.lastname: Chen
authors.affiliation: Department of Obstetric and Gynecologic diseases, West China Second University Hospital, Sichuan University, Chengdu 610041, P.R. China.|SCU-CUHK Joint Laboratory of Reproductive Medicine, Key Laboratory of Birth Defects and Related Diseases of Women and Children, Ministry of Education, (Sichuan University), West China Second University Hospital, Sichuan University, Chengdu 610041, P.R. China.
authors.forename: W M
authors.initials: WM
authors.lastname: Xu
authors.affiliation: Department of Obstetric and Gynecologic diseases, West China Second University Hospital, Sichuan University, Chengdu 610041, P.R. China.|SCU-CUHK Joint Laboratory of Reproductive Medicine, Key Laboratory of Birth Defects and Related Diseases of Women and Children, Ministry of Education, (Sichuan University), West China Second University Hospital, Sichuan University, Chengdu 610041, P.R. China.
authors.forename: M
authors.initials: M
authors.lastname: Yang
authors.affiliation: Department of Obstetric and Gynecologic diseases, West China Second University Hospital, Sichuan University, Chengdu 610041, P.R. China.|SCU-CUHK Joint Laboratory of Reproductive Medicine, Key Laboratory of Birth Defects and Related Diseases of Women and Children, Ministry of Education, (Sichuan University), West China Second University Hospital, Sichuan University, Chengdu 610041, P.R. China.
authors.forename: K
authors.initials: K
authors.lastname: Wang
authors.affiliation: Department of Obstetric and Gynecologic diseases, West China Second University Hospital, Sichuan University, Chengdu 610041, P.R. China.|SCU-CUHK Joint Laboratory of Reproductive Medicine, Key Laboratory of Birth Defects and Related Diseases of Women and Children, Ministry of Education, (Sichuan University), West China Second University Hospital, Sichuan University, Chengdu 610041, P.R. China.
authors.forename: Y
authors.initials: Y
authors.lastname: Chen
authors.affiliation: College of Animal Science &Technology, Nanjing Agriculture University, Nanjing, China.
authors.forename: X J
authors.initials: XJ
authors.lastname: Huang
authors.affiliation: Department of Obstetric and Gynecologic diseases, West China Second University Hospital, Sichuan University, Chengdu 610041, P.R. China.|SCU-CUHK Joint Laboratory of Reproductive Medicine, Key Laboratory of Birth Defects and Related Diseases of Women and Children, Ministry of Education, (Sichuan University), West China Second University Hospital, Sichuan University, Chengdu 610041, P.R. China.
authors.forename: Q H
authors.initials: QH
authors.lastname: Ma
mesh_terms: D000022:Abortion, Spontaneous / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D000818:Animals; D017209:Apoptosis; D001755:Blastocyst / Q000378:metabolism* / Q000473:pathology; D047108:Embryonic Development; D005260:Female; D018507:Gene Expression Regulation, Developmental; D055785:Gene Knockdown Techniques; D006801:Humans; D051379:Mice; D008813:Mice, Inbred ICR; D011247:Pregnancy; D025521:Tumor Suppressor Proteins / Q000096:biosynthesis* / Q000235:genetics; D044767:Ubiquitin-Protein Ligases / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D025521:Tumor Suppressor Proteins; C502830:HUWE1 protein, human; C502831:Huwe1 protein, mouse; D044767:Ubiquitin-Protein Ligases