title: SandTraps are efficient, scalable, and mild systems for harvesting, washing and concentrating cells.
abstract: Microbial cells vary widely in size, specific density, shearing resistance, oxygen sensitivity and abundance so that differential harvesting and washing procedures are needed to efficiently recover cells from dilute suspensions. We here describe a mild, simple, variable and cost-efficient method to harvest cells on columns packed with silica beads. The method collects and concentrates 40-98% of the cells preserving enzymatic activity and cell viability. The method can be applied for strictly anaerobic microorganisms, is scalable to different culture volumes and can be multiplexed in standardized systems. We see major application potential in harvesting small cells leaking through 0.2μm filters, for harvesting strictly anaerobic cells and for differential harvesting of cells according to cell size using a gradient system.
authors.affiliation: Helmholtz Centre for Environmental Research - UFZ, Department Isotope Biogeochemistry, Permoserstraße 15, 04318 Leipzig, Germany.
authors.forename: Danny
authors.initials: D
authors.lastname: Frauenstein
authors.affiliation: Helmholtz Centre for Environmental Research - UFZ, Department Isotope Biogeochemistry, Permoserstraße 15, 04318 Leipzig, Germany.
authors.forename: Katja
authors.initials: K
authors.lastname: Seidel
authors.affiliation: Helmholtz Centre for Environmental Research - UFZ, Department Isotope Biogeochemistry, Permoserstraße 15, 04318 Leipzig, Germany. Electronic address: lorenz.adrian@ufz.de.
authors.forename: Lorenz
authors.initials: L
authors.lastname: Adrian
mesh_terms: D001431:Bacteriological Techniques / Q000295:instrumentation* / Q000379:methods; D041862:Chloroflexi / Q000302:isolation & purification*; D015169:Colony Count, Microbial; D050296:Microbial Viability; D012822:Silicon Dioxide / Q000737:chemistry
chemical_list: D012822:Silicon Dioxide
keywords: Anaerobic microbiology; Cell concentration; Cell harvesting; Cell washing; Dehalococcoides; Nanobacteria; Silica gel; Ultramicrobacteria