title: Multilocus sequence type profiles of Bacillus cereus isolates from infant formula in China.
abstract: Bacillus cereus sensu stricto is an opportunistic foodborne pathogen. The multilocus sequence type (MLST) of 74 B. cereus isolated from 513 non-random infant formula in China was analyzed. Of 64 sequence types (STs) detected, 50 STs and 6 alleles were newly found in PubMLST database. All isolates except for one singleton (ST-1049), were classified into 7 clonal complexes (CC) by BURST (n-4), in which CC1 with core ancestral clone ST-26 was the largest group including 86% isolates, and CC2, 3, 9, 10 and 13 were first reported in China. MLST profiles of the isolates from 8 infant formula brands were compared. It was found the brands might be potentially tracked by the variety of STs, such as ST-1049 of singleton and ST-1062 of isolate from goat milk source, though they could not be easily tracked just by clonal complex types of the isolates.
authors.affiliation: Department of Microbiology, Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou, Zhejiang 310051, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Yang
authors.affiliation: College of Food Science, Shihezi University, Shihezi, Xinjiang 832001, China.
authors.forename: Xiaofeng
authors.initials: X
authors.lastname: Yu
authors.affiliation: Department of Microbiology, Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou, Zhejiang 310051, China.
authors.forename: Li
authors.initials: L
authors.lastname: Zhan
authors.affiliation: Department of Microbiology, Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou, Zhejiang 310051, China.
authors.forename: Jiancai
authors.initials: J
authors.lastname: Chen
authors.affiliation: Department of Microbiology, Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou, Zhejiang 310051, China.
authors.forename: Yunyi
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Microbiology, Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou, Zhejiang 310051, China.
authors.forename: Junyan
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Department of Microbiology, Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou, Zhejiang 310051, China.
authors.forename: Honghu
authors.initials: H
authors.lastname: Chen
authors.affiliation: Department of Microbiology, Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou, Zhejiang 310051, China.
authors.forename: Zheng
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: Department of Microbiology, Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou, Zhejiang 310051, China.
authors.forename: Yanjun
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Microbiology, Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou, Zhejiang 310051, China.
authors.forename: Yiyu
authors.initials: Y
authors.lastname: Lu
authors.affiliation: Department of Microbiology, Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou, Zhejiang 310051, China. Electronic address: llmei@cdc.zj.cn.
authors.forename: Lingling
authors.initials: L
authors.lastname: Mei
mesh_terms: D000483:Alleles; D000818:Animals; D001409:Bacillus cereus / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D002681:China; D004269:DNA, Bacterial; D005838:Genotype; D006801:Humans; D007223:Infant; D041943:Infant Formula / Q000382:microbiology*; D008892:Milk / Q000382:microbiology*; D058885:Multilocus Sequence Typing / Q000379:methods; D011358:Product Surveillance, Postmarketing
chemical_list: D004269:DNA, Bacterial
keywords: Bacillus cereus; Clonal complex; Infant formula; MLST; Surveillance