title: Identification of Shared and Unique Serum Lipid Profiles in Diabetes Mellitus and Myocardial Infarction.
abstract: Diabetes mellitus (DM) and cardiovascular disease are associated with dyslipidemia, but the detailed lipid molecular pattern in both diseases remains unknown.
  
  
  
  We used shotgun mass spectrometry to determine serum levels of 255 molecular lipids in 316 controls, 171 DM, and 99 myocardial infarction (MI) events from a cohort derived from the Malmö Diet and Cancer study. Orthogonal projections to latent structures analyses were conducted between the lipids and clinical parameters describing DM or MI. Fatty acid desaturases (FADS) and elongation of very long chain fatty acid protein 5 (ELOVL5) activities were estimated by calculating product to precursor ratios of polyunsaturated fatty acids in complex lipids. FADS genotypes encoding these desaturases were then tested for association with lipid levels and ratios. Differences in the levels of lipids belonging to the phosphatidylcholine and triacylglyceride (TAG) classes contributed the most to separating DM from controls. TAGs also played a dominating role in discriminating MI from controls. Levels of C18:2 fatty acids in complex lipids were lower both in DM and MI versus controls (DM, P=0.004; MI, P=6.0E-06) at least due to an acceleration in the metabolic flux from C18:2 to C20:4 (eg, increased estimated ELOVL5: DM, P=0.02; MI, P=0.04, and combined elongase-desaturase activities: DM, P=3.0E-06; MI, P=2.0E-06). Minor allele carriers of FADS genotypes were associated with increased levels of C18:2 (P≤0.007) and lower desaturase activity (P≤0.002).
  
  
  
  We demonstrate a possible relationship between decreased levels of C18:2 in complex lipids and DM or MI. We thereby highlight the importance of molecular lipids in the pathogenesis of both diseases.
authors.affiliation: Science for Life Laboratory, Department of Biochemistry and Biophysics, Stockholm University, Stockholm, Sweden.
authors.forename: Sanela
authors.initials: S
authors.lastname: Kjellqvist
authors.affiliation: Lipotype GmbH, Dresden, Germany.
authors.forename: Christian
authors.initials: C
authors.lastname: Klose
authors.affiliation: Lipotype GmbH, Dresden, Germany.
authors.forename: Michal A
authors.initials: MA
authors.lastname: Surma
authors.affiliation: Department of Clinical Sciences, Lund University, Malmö, Sweden.
authors.forename: George
authors.initials: G
authors.lastname: Hindy
authors.affiliation: Department of Clinical Sciences, Lund University, Malmö, Sweden.
authors.forename: Inês G
authors.initials: IG
authors.lastname: Mollet
authors.affiliation: Science for Life Laboratory, Department of Biochemistry and Biophysics, Stockholm University, Stockholm, Sweden.
authors.forename: Anna
authors.initials: A
authors.lastname: Johansson
authors.affiliation: Department of Nutrition, Faculté de Médecine et de Pharmacie de la Timone, Aix Marseille University, Marseille, France.
authors.forename: Patrick
authors.initials: P
authors.lastname: Chavaux
authors.affiliation: Department of Chemistry and Molecular Biology, University of Gothenburg, Sweden.
authors.forename: Johan
authors.initials: J
authors.lastname: Gottfries
authors.affiliation: Lipotype GmbH, Dresden, Germany.
authors.forename: Kai
authors.initials: K
authors.lastname: Simons
authors.affiliation: Department of Clinical Sciences, Lund University, Malmö, Sweden.
authors.forename: Olle
authors.initials: O
authors.lastname: Melander
authors.affiliation: Department of Clinical Sciences, Lund University, Malmö, Sweden celine.fernandez@med.lu.se.
authors.forename: Céline
authors.initials: C
authors.lastname: Fernandez
mesh_terms: D000123:Acetyltransferases / Q000378:metabolism; D000368:Aged; D016022:Case-Control Studies; D003924:Diabetes Mellitus, Type 2 / Q000097:blood* / Q000235:genetics; D044943:Fatty Acid Desaturases / Q000235:genetics / Q000378:metabolism; D000081031:Fatty Acid Elongases; D005231:Fatty Acids, Unsaturated / Q000378:metabolism; D005260:Female; D005838:Genotype; D006801:Humans; D050356:Lipid Metabolism / Q000235:genetics / Q000502:physiology*; D008297:Male; D013058:Mass Spectrometry; D008875:Middle Aged; D009203:Myocardial Infarction / Q000097:blood* / Q000235:genetics; D020641:Polymorphism, Single Nucleotide / Q000235:genetics
chemical_list: C000630954:ELOVL5 protein, human; D005231:Fatty Acids, Unsaturated; D044943:Fatty Acid Desaturases; D000123:Acetyltransferases; D000081031:Fatty Acid Elongases
keywords: diabetes mellitus; fatty acid desaturase; genotype; lipid metabolites; myocardial infarction