title: Implantation of placenta-derived mesenchymal stem cells accelerates murine dermal wound closure through immunomodulation.
abstract: BACKGROUND
  Diabetic foot ulcer (DFU) is a major complication of diabetes mellitus. Although previous studies have established that inflammation, ischemia and neuropathy contribute to the development of DFU, it is still an unmet medical need due to lack knowledge of cellular and molecular mechanisms associated with DFU. In the present study, we tested our hypothesis that subcutaneous application of human placental mesenchymal stem cells (PMSCs) can accelerate diabetic dermal wound healing by modulating immunoresponse.
  
  
  RESULTS
  By using anin vivoexcisional wound healing model in Goto-Kakizaki (GK) rats, we found that injection of PMSCs accelerates wound closure. Further studies revealed that application of PMSCs can regulate inflammation associated with wound healing by controlling secretion of pro- and anti-inflammatory factors, the beneficial effects can be partially blocked by application of antibodies against interleukin-10 (IL-10). Furthermore,in vitroexperiments suggested that co-culture of PMSCs with human dermal fibroblasts can significantly inhibit activation of NF-ĸB induced by lipopolysaccharides (LPS), indicating the molecular mechanism of PMSCs mediated immunomodulation.
  
  
  CONCLUSIONS
  Taken together, our study suggested that the immunomodulation of PMSCs play an important role on diabetic dermal wound healing process, thus PMSCs might represent an attractive choice for treatment of diabetes dermal wound and DFU.
authors.affiliation: Division of Geriatrics, Tongji Hospital, School of Medicine, Tongji University Shanghai 200065, China.
authors.forename: Haifeng
authors.initials: H
authors.lastname: Wang
authors.affiliation: Department of Integrative Oncology, Fudan University Shanghai Cancer CenterShanghai 200032, China; Department of Oncology, Shanghai Medical College, Fudan UniversityShanghai 200032, China.
authors.forename: Lianyu
authors.initials: L
authors.lastname: Chen
authors.affiliation: Division of Geriatrics, Tongji Hospital, School of Medicine, Tongji University Shanghai 200065, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Division of Geriatrics, Tongji Hospital, School of Medicine, Tongji University Shanghai 200065, China.
authors.forename: Bangzhen
authors.initials: B
authors.lastname: Luo
authors.affiliation: Division of Geriatrics, Tongji Hospital, School of Medicine, Tongji University Shanghai 200065, China.
authors.forename: Nanzi
authors.initials: N
authors.lastname: Xie
authors.affiliation: Department of Surgery, Davis Heart and Lung Research Institute, The Ohio State University Wexner Medical Center Columbus, Ohio 43210, USA.
authors.forename: Tao
authors.initials: T
authors.lastname: Tan
authors.affiliation: Department of Endocrinology, Tongji Hospital, Tongji University School of Medicine Shanghai 200065, China.
authors.forename: Lige
authors.initials: L
authors.lastname: Song
authors.affiliation: Department of General Surgery, Tongji University Yangpu Hospital, Institute of Gastrointestinal Surgery and Translational Medicine, Tongji University School of Medicine Shanghai 200065, China.
authors.forename: Pei
authors.initials: P
authors.lastname: Erli
authors.affiliation: Division of Geriatrics, Tongji Hospital, School of Medicine, Tongji University Shanghai 200065, China.
authors.forename: Ming
authors.initials: M
authors.lastname: Luo
keywords: Diabetic foot ulcer; placenta-derived mesenchymal stem cell; wound closure