title: Dendrimer-Stabilized Gold Nanostars as a Multifunctional Theranostic Nanoplatform for CT Imaging, Photothermal Therapy, and Gene Silencing of Tumors.
abstract: Development of versatile nanomaterials combining diagnostic and therapeutic functionalities within one single nanoplatform is extremely important for tumor theranostics. In this work, the authors report the synthesis of a gold nanostar (Au NS)-based theranostic platform stabilized with cyclic arginine-glycine-aspartic (Arg-Gly-Asp, RGD) peptide-modified amine-terminated generation 3 poly(amidoamine) dendrimers. The formed RGD-modified dendrimer-stabilized Au NSs (RGD-Au DSNSs) are used as a gene delivery vector to complex small interfering RNA (siRNA) for computed tomography (CT) imaging, thermal imaging, photothermal therapy (PTT), and gene therapy of tumors. The results show that the RGD-Au DSNSs are able to compact vascular endothelial growth factor siRNA and specifically deliver siRNA to cancer cells overexpressing αvβ3integrin. Under near-infrared laser irradiation, the viability of cancer cells is only 20.2% after incubation with the RGD-Au DSNS/siRNA polyplexes, which is much lower than that of cells after single PTT or gene therapy treatment. Furthermore, in vivo results show that the RGD-Au DSNS/siRNA polyplexes enable tumor CT imaging, thermal imaging, PTT, and gene therapy after intratumoral injection. These results indicate that the developed multifunctional nanoconstruct is a promising platform for tumor imaging and combinational PTT and gene therapy.
authors.affiliation: State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai, 201620, P. R. China.
authors.forename: Ping
authors.initials: P
authors.lastname: Wei
authors.affiliation: Department of Radiology, Shanghai General Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai, 200080, P. R. China.
authors.forename: Jingwen
authors.initials: J
authors.lastname: Chen
authors.affiliation: State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai, 201620, P. R. China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Hu
authors.affiliation: State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai, 201620, P. R. China.
authors.forename: Xin
authors.initials: X
authors.lastname: Li
authors.affiliation: Department of Radiology, Shanghai General Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai, 200080, P. R. China.
authors.forename: Han
authors.initials: H
authors.lastname: Wang
authors.affiliation: State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai, 201620, P. R. China.
authors.forename: Mingwu
authors.initials: M
authors.lastname: Shen
authors.affiliation: State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai, 201620, P. R. China.|CQM-Centro de Quimica da Madeira, Universidade da Madeira, Campus da Penteada, Funchal, 9000-390, Portugal.
authors.forename: Xiangyang
authors.initials: X
authors.lastname: Shi
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D050091:Dendrimers / Q000008:administration & dosage*; D020868:Gene Silencing / Q000187:drug effects*; D006046:Gold / Q000008:administration & dosage*; D006801:Humans; D007259:Infrared Rays; D039302:Integrin alphaVbeta3 / Q000378:metabolism; D053768:Metal Nanoparticles / Q000008:administration & dosage*; D051379:Mice; D049329:Nanostructures / Q000008:administration & dosage; D009369:Neoplasms / Q000188:drug therapy* / Q000378:metabolism; D009842:Oligopeptides / Q000008:administration & dosage; D010789:Phototherapy / Q000379:methods; D034741:RNA, Small Interfering / Q000008:administration & dosage; D000068936:Theranostic Nanomedicine / Q000379:methods; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism
chemical_list: D050091:Dendrimers; D039302:Integrin alphaVbeta3; D009842:Oligopeptides; D034741:RNA, Small Interfering; D042461:Vascular Endothelial Growth Factor A; D006046:Gold; C047981:arginyl-glycyl-aspartic acid
keywords: dendrimers; gene silencing; gold nanostars; theranostics; tumors