title: Neutrophil infiltration mediated by CXCL5 accumulation in the laryngeal squamous cell carcinoma microenvironment: A mechanism by which tumour cells escape immune surveillance.
abstract: The CXCL5 chemokine is important for neutrophil accumulation in tumour tissues. In this report, we attempted to clarify whether and how infiltrating tumour-associated neutrophils (TANs) in laryngeal squamous cell carcinoma (LSCC) affect the proliferation and activation of T cells. We examined chemokine expression by real-time PCR (RT-PCR) and enzyme-linked immunosorbent assay (ELISA) and performed an immunohistochemical analysis of LSCC microarrays. The relationship between CXCL5 and CD66b (a neutrophil marker) was investigated by immunofluorescence staining. We found that CXCL5 was upregulated in LSCC tissues, whereas CXCL5 levels were decreased in LSCC patient serum. Furthermore, high levels of CXCL5 were significantly correlated with intratumoural neutrophil infiltration. Compared with peripheral blood neutrophils (PBNs), TANs significantly inhibited T cell proliferation and decreased IFN-γ and TNF-α secretion. These data suggest that excessive neutrophil infiltration is associated with advanced clinical stages of LSCC (T3 or T4, III or IV, and N1 or N2).
authors.affiliation: Department of Otolaryngology-HNS, Eye, Ear, Nose and Throat Hospital, Shanghai Key Clinical Disciplines of Otorhinolaryngology, Fudan University School of Medicine, 83 Fenyang Road, Shanghai 200031, China; Department of Pudong Hospital, Fudan University School of Medicine, 2800 Gongwei Road, Shanghai 201300, China.
authors.forename: Duo
authors.initials: D
authors.lastname: Zhang
authors.affiliation: Department of Otolaryngology-HNS, Eye, Ear, Nose and Throat Hospital, Shanghai Key Clinical Disciplines of Otorhinolaryngology, Fudan University School of Medicine, 83 Fenyang Road, Shanghai 200031, China; Department of Pudong Hospital, Fudan University School of Medicine, 2800 Gongwei Road, Shanghai 201300, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Zhou
authors.affiliation: Department of Otolaryngology-HNS, Eye, Ear, Nose and Throat Hospital, Shanghai Key Clinical Disciplines of Otorhinolaryngology, Fudan University School of Medicine, 83 Fenyang Road, Shanghai 200031, China; Department of Pudong Hospital, Fudan University School of Medicine, 2800 Gongwei Road, Shanghai 201300, China.
authors.forename: Di
authors.initials: D
authors.lastname: Tang
authors.affiliation: Shanghai Institute of Immunology, Shanghai Jiaotong University School of Medicine, 280 South Chongqing Road, Shanghai 200025, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Zhou
authors.affiliation: Department of Otolaryngology-HNS, Eye, Ear, Nose and Throat Hospital, Shanghai Key Clinical Disciplines of Otorhinolaryngology, Fudan University School of Medicine, 83 Fenyang Road, Shanghai 200031, China; Department of Pudong Hospital, Fudan University School of Medicine, 2800 Gongwei Road, Shanghai 201300, China.
authors.forename: Liang
authors.initials: L
authors.lastname: Chou
authors.affiliation: Shanghai Institute of Immunology, Shanghai Jiaotong University School of Medicine, 280 South Chongqing Road, Shanghai 200025, China.
authors.forename: Kuang-Yen
authors.initials: KY
authors.lastname: Chou
authors.affiliation: Department of Otolaryngology-HNS, Eye, Ear, Nose and Throat Hospital, Shanghai Key Clinical Disciplines of Otorhinolaryngology, Fudan University School of Medicine, 83 Fenyang Road, Shanghai 200031, China; Department of Pudong Hospital, Fudan University School of Medicine, 2800 Gongwei Road, Shanghai 201300, China. Electronic address: doctortaolei@163.com.
authors.forename: Lei
authors.initials: L
authors.lastname: Tao
authors.affiliation: Shanghai Institute of Immunology, Shanghai Jiaotong University School of Medicine, 280 South Chongqing Road, Shanghai 200025, China. Electronic address: lulunew2003@163.com.
authors.forename: Li-Ming
authors.initials: LM
authors.lastname: Lu
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002294:Carcinoma, Squamous Cell / Q000097:blood / Q000276:immunology* / Q000378:metabolism; D049109:Cell Proliferation / Q000502:physiology; D054365:Chemokine CXCL5 / Q000097:blood / Q000378:metabolism*; D005260:Female; D006258:Head and Neck Neoplasms / Q000097:blood / Q000276:immunology / Q000378:metabolism; D006801:Humans; D007822:Laryngeal Neoplasms / Q000097:blood / Q000276:immunology* / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D020556:Neutrophil Infiltration / Q000276:immunology*; D009504:Neutrophils / Q000276:immunology* / Q000378:metabolism; D000077195:Squamous Cell Carcinoma of Head and Neck; D013601:T-Lymphocytes / Q000276:immunology / Q000378:metabolism; D059016:Tumor Microenvironment / Q000276:immunology*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism; D015854:Up-Regulation / Q000276:immunology
chemical_list: C486263:CXCL5 protein, human; D054365:Chemokine CXCL5; D014409:Tumor Necrosis Factor-alpha
keywords: CXCL5; LSCC; Neutrophil; PBNs; TANs; Tumour microenvironment