title: Cancer Secretome May Influence BSP and DSP Expression in Human Salivary Gland Cells.
abstract: One of the biggest challenges in managing head and neck cancers, especially salivary gland cancers, is the identification of secreted biomarkers of the disease that can be evaluated noninvasively. A relevant source of enriched tumor markers could potentially be found in the tumor secretome. Although numerous studies have evaluated secretomes from various cancers, the influence of the cancer secretome derived from salivary gland cancers on the behavior of normal cells has not yet been elucidated. Our data indicate that secretome derived from salivary gland cancer cells can influence the expression of two potential biomarkers of oral cancer-namely, bone sialoprotein (BSP) and dentin sialoprotein (DSP)-in normal salivary gland cells. Using routine immunohistochemistry, immunofluorescence, and immunoblotting techniques, we demonstrate an enrichment of BSP and DSP in human salivary gland (HSG) cancer tissue, unique localizations of BSP and DSP in HSG cancer cells, and enriched expression of BSP and DSP in normal salivary gland cells exposed to a cancer secretome. The secretome domain of the cancer microenvironment could alter signaling cascades responsible for normal cell proliferation, migration, and invasion, thus enhancing cancer cell survival and the potential for cancer progression. The cancer secretome may be critical in maintaining and stimulating "cancer-ness," thus potentially promoting specific hallmarks of metastasis.
authors.affiliation: Department of Growth, Development and Structure, Southern Illinois University School of Dental Medicine, Alton, Illinois (SLH, BF, ASE, JCY, ARJ).|Department of Biological Sciences, College of Arts and Sciences, Southern Illinois University Edwardsville, Edwardsville, Illinois (SLH, JCY, ARJ).
authors.forename: Samantha Lynn
authors.initials: SL
authors.lastname: Hamilton
authors.affiliation: Department of Growth, Development and Structure, Southern Illinois University School of Dental Medicine, Alton, Illinois (SLH, BF, ASE, JCY, ARJ).
authors.forename: Blake
authors.initials: B
authors.lastname: Ferando
authors.affiliation: Department of Growth, Development and Structure, Southern Illinois University School of Dental Medicine, Alton, Illinois (SLH, BF, ASE, JCY, ARJ).
authors.forename: Asha Sarah
authors.initials: AS
authors.lastname: Eapen
authors.affiliation: Department of Growth, Development and Structure, Southern Illinois University School of Dental Medicine, Alton, Illinois (SLH, BF, ASE, JCY, ARJ).|Department of Biological Sciences, College of Arts and Sciences, Southern Illinois University Edwardsville, Edwardsville, Illinois (SLH, JCY, ARJ).
authors.forename: Jennifer Chian
authors.initials: JC
authors.lastname: Yu
authors.affiliation: Department of Growth, Development and Structure, Southern Illinois University School of Dental Medicine, Alton, Illinois (SLH, BF, ASE, JCY, ARJ).|Department of Biological Sciences, College of Arts and Sciences, Southern Illinois University Edwardsville, Edwardsville, Illinois (SLH, JCY, ARJ).
authors.forename: Anita Rose
authors.initials: AR
authors.lastname: Joy
mesh_terms: D002460:Cell Line; D045744:Cell Line, Tumor; D016326:Extracellular Matrix Proteins / Q000032:analysis* / Q000378:metabolism; D006801:Humans; D058574:Integrin-Binding Sialoprotein / Q000032:analysis* / Q000378:metabolism; D010750:Phosphoproteins / Q000032:analysis* / Q000378:metabolism; D012468:Salivary Gland Neoplasms / Q000378:metabolism / Q000473:pathology*; D012469:Salivary Glands / Q000166:cytology / Q000378:metabolism / Q000473:pathology*; D012795:Sialoglycoproteins / Q000032:analysis* / Q000378:metabolism; D000099311:Dentin Sialophosphoprotein
chemical_list: D016326:Extracellular Matrix Proteins; D058574:Integrin-Binding Sialoprotein; D010750:Phosphoproteins; D012795:Sialoglycoproteins; D000099311:Dentin Sialophosphoprotein
keywords: bone sialoprotein; cancer secretome; dentin sialoprotein; salivary gland