title: Bph32, a novel gene encoding an unknown SCR domain-containing protein, confers resistance against the brown planthopper in rice.
abstract: An urgent need exists to identify more brown planthopper (Nilaparvata lugens Stål, BPH) resistance genes, which will allow the development of rice varieties with resistance to BPH to counteract the increased incidence of this pest species. Here, using bioinformatics and DNA sequencing approaches, we identified a novel BPH resistance gene, LOC_Os06g03240 (MSU LOCUS ID), from the rice variety Ptb33 in the interval between the markers RM19291 and RM8072 on the short arm of chromosome 6, where a gene for resistance to BPH was mapped by Jirapong Jairin et al. and renamed as "Bph32". This gene encodes a unique short consensus repeat (SCR) domain protein. Sequence comparison revealed that the Bph32 gene shares 100% sequence identity with its allele in Oryza latifolia. The transgenic introgression of Bph32 into a susceptible rice variety significantly improved resistance to BPH. Expression analysis revealed that Bph32 was highly expressed in the leaf sheaths, where BPH primarily settles and feeds, at 2 and 24 h after BPH infestation, suggesting that Bph32 may inhibit feeding in BPH. Western blotting revealed the presence of Pph (Ptb33) and Tph (TN1) proteins using a Penta-His antibody, and both proteins were insoluble. This study provides information regarding a valuable gene for rice defence against insect pests.
authors.affiliation: Crop Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu, 610066, P.R. China.
authors.forename: Juansheng
authors.initials: J
authors.lastname: Ren
authors.affiliation: Crop Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu, 610066, P.R. China.
authors.forename: Fangyuan
authors.initials: F
authors.lastname: Gao
authors.affiliation: Crop Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu, 610066, P.R. China.
authors.forename: Xianting
authors.initials: X
authors.lastname: Wu
authors.affiliation: Crop Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu, 610066, P.R. China.
authors.forename: Xianjun
authors.initials: X
authors.lastname: Lu
authors.affiliation: Sichuan Normal University, Chengdu, 610066, P.R. China.
authors.forename: Lihua
authors.initials: L
authors.lastname: Zeng
authors.affiliation: Crop Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu, 610066, P.R. China.
authors.forename: Jianqun
authors.initials: J
authors.lastname: Lv
authors.affiliation: Crop Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu, 610066, P.R. China.
authors.forename: Xiangwen
authors.initials: X
authors.lastname: Su
authors.affiliation: Department of Genetics and Biochemistry, Clemson University, 110 Biosystems Research Complex, Clemson, SC 29634-0318, USA.
authors.forename: Hong
authors.initials: H
authors.lastname: Luo
authors.affiliation: Crop Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu, 610066, P.R. China.
authors.forename: Guangjun
authors.initials: G
authors.lastname: Ren
mesh_terms: D000483:Alleles; D000818:Animals; D001483:Base Sequence; D019295:Computational Biology; D060467:Disease Resistance; D004352:Drug Resistance, Microbial; D060146:Ecotype; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D005816:Genetic Complementation Test; D005838:Genotype; D006430:Hemiptera / Q000502:physiology*; D018895:Microsatellite Repeats / Q000235:genetics; D012275:Oryza / Q000235:genetics* / Q000469:parasitology*; D010641:Phenotype; D010802:Phylogeny; D010935:Plant Diseases / Q000235:genetics* / Q000469:parasitology*; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D030821:Plants, Genetically Modified; D000072417:Protein Domains; D017422:Sequence Analysis, DNA; D013347:Subcellular Fractions / Q000378:metabolism
chemical_list: D010940:Plant Proteins