title: TruePrime is a novel method for whole-genome amplification from single cells based on TthPrimPol.
abstract: Sequencing of a single-cell genome requires DNA amplification, a process prone to introducing bias and errors into the amplified genome. Here we introduce a novel multiple displacement amplification (MDA) method based on the unique DNA primase features of Thermus thermophilus (Tth) PrimPol. TthPrimPol displays a potent primase activity preferring dNTPs as substrates unlike conventional primases. A combination of TthPrimPol's unique ability to synthesize DNA primers with the highly processive Phi29 DNA polymerase (Φ29DNApol) enables near-complete whole genome amplification from single cells. This novel method demonstrates superior breadth and evenness of genome coverage, high reproducibility, excellent single-nucleotide variant (SNV) detection rates with low allelic dropout (ADO) and low chimera formation as exemplified by sequencing HEK293 cells. Moreover, copy number variant (CNV) calling yields superior results compared with random primer-based MDA methods. The advantages of this method, which we named TruePrime, promise to facilitate and improve single-cell genomic analysis.
authors.affiliation: SYGNIS Biotech S.L.U., Parque Científico de Madrid, Cantoblanco, Madrid 28049, Spain.
authors.forename: Ángel J
authors.initials: ÁJ
authors.lastname: Picher
authors.affiliation: SYGNIS Bioscience GmbH, Waldhofer Strasse 104, Heidelberg 69123, Germany.
authors.forename: Bettina
authors.initials: B
authors.lastname: Budeus
authors.affiliation: SYGNIS Bioscience GmbH, Waldhofer Strasse 104, Heidelberg 69123, Germany.
authors.forename: Oliver
authors.identifier: 0000-0002-7430-0992
authors.initials: O
authors.lastname: Wafzig
authors.affiliation: SYGNIS Bioscience GmbH, Waldhofer Strasse 104, Heidelberg 69123, Germany.
authors.forename: Carola
authors.initials: C
authors.lastname: Krüger
authors.affiliation: Centro de Biología Molecular Severo Ochoa (CSIC-UAM), Cantoblanco, Madrid 28049, Spain.
authors.forename: Sara
authors.initials: S
authors.lastname: García-Gómez
authors.affiliation: Centro de Biología Molecular Severo Ochoa (CSIC-UAM), Cantoblanco, Madrid 28049, Spain.
authors.forename: María I
authors.initials: MI
authors.lastname: Martínez-Jiménez
authors.affiliation: Centro de Biología Molecular Severo Ochoa (CSIC-UAM), Cantoblanco, Madrid 28049, Spain.
authors.forename: Alberto
authors.initials: A
authors.lastname: Díaz-Talavera
authors.affiliation: SYGNIS Bioscience GmbH, Waldhofer Strasse 104, Heidelberg 69123, Germany.
authors.forename: Daniela
authors.initials: D
authors.lastname: Weber
authors.affiliation: Centro de Biología Molecular Severo Ochoa (CSIC-UAM), Cantoblanco, Madrid 28049, Spain.
authors.forename: Luis
authors.initials: L
authors.lastname: Blanco
authors.affiliation: SYGNIS Bioscience GmbH, Waldhofer Strasse 104, Heidelberg 69123, Germany.
authors.forename: Armin
authors.initials: A
authors.lastname: Schneider
mesh_terms: D000483:Alleles; D000595:Amino Acid Sequence; D001483:Base Sequence; D004247:DNA / Q000235:genetics; D056915:DNA Copy Number Variations / Q000235:genetics; D019915:DNA Primase / Q000737:chemistry / Q000378:metabolism; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism; D015894:Genome, Human; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*; D015203:Reproducibility of Results; D017422:Sequence Analysis, DNA; D059010:Single-Cell Analysis; D013698:Templates, Genetic; D016963:Thermus thermophilus / Q000201:enzymology
chemical_list: D004247:DNA; D019915:DNA Primase; D004259:DNA-Directed DNA Polymerase