title: Tailoring shape and size of biogenic silver nanoparticles to enhance antimicrobial efficacy against MDR bacteria.
abstract: Spherical, rectangular, penta, and hexagonal silver nanoparticles of different dimensions were biosynthesized in an eco-friendly manner by biocontrol agent, Trichoderma viride by manipulating physical parameters, pH, temperature, and reaction time. The particles were characterized by UV-vis spectroscopy; Dynamic Light Scattering (DLS), Transmission Electron Microscopy (TEM) and Fourier Transform Infra-red Spectroscopy (FTIR). Shape and size dependent antimicrobial activity of nanoparticles against human pathogens was observed. Maximum inhibition was found with spherical nanoparticles (2-5 nm) showing 40, 51, 43, 53.9 and 55.8% against Shigella sonnei, Escherichia coli, Serratia marcescens, Staphylococcus. aureus and Pseudomonas aeruginosa respectively, where as pentagonal and hexagonal nanoparticles (50-100 nm) demonstrated 32, 41, 31, 42.84 and 42.80% of inhibition as compared to control. Nanoparticles of different geometry and dimension established enhanced antagonistic activity against pathogens with all the tested antibiotics. Excellent antimicrobial efficacy was obtained with spherical nanoparticles of 2-5 nm with ampicillin and penicillin. Shape and size played major role in enhancing antimicrobial potential of silver nanoparticles, both singly and synergistically with antibiotics which can be exploited to combat the spread of multidrug resistant pathogens.
authors.affiliation: CSIR-National Botanical Research Institute, Rana Pratap Marg, Lucknow, 226 001, India.
authors.forename: Madhuree
authors.initials: M
authors.lastname: Kumari
authors.affiliation: CSIR-National Botanical Research Institute, Rana Pratap Marg, Lucknow, 226 001, India.
authors.forename: Shipra
authors.initials: S
authors.lastname: Pandey
authors.affiliation: CSIR-National Botanical Research Institute, Rana Pratap Marg, Lucknow, 226 001, India.
authors.forename: Ved Prakash
authors.initials: VP
authors.lastname: Giri
authors.affiliation: CSIR-National Botanical Research Institute, Rana Pratap Marg, Lucknow, 226 001, India.
authors.forename: Arpita
authors.initials: A
authors.lastname: Bhattacharya
authors.affiliation: CSIR-National Botanical Research Institute, Rana Pratap Marg, Lucknow, 226 001, India.
authors.forename: Richa
authors.initials: R
authors.lastname: Shukla
authors.affiliation: CSIR-National Botanical Research Institute, Rana Pratap Marg, Lucknow, 226 001, India. Electronic address: mishramyco@yahoo.com.
authors.forename: Aradhana
authors.initials: A
authors.lastname: Mishra
authors.affiliation: CSIR-National Botanical Research Institute, Rana Pratap Marg, Lucknow, 226 001, India.
authors.forename: C S
authors.initials: CS
authors.lastname: Nautiyal
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry* / Q000494:pharmacology*; D004357:Drug Synergism; D004926:Escherichia coli / Q000187:drug effects; D006090:Gram-Negative Bacteria / Q000187:drug effects; D006094:Gram-Positive Bacteria / Q000187:drug effects; D006801:Humans; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000627:therapeutic use*; D008826:Microbial Sensitivity Tests; D011550:Pseudomonas aeruginosa / Q000187:drug effects; D012764:Shigella sonnei / Q000187:drug effects; D012834:Silver / Q000737:chemistry* / Q000494:pharmacology*; D013211:Staphylococcus aureus / Q000187:drug effects
chemical_list: D000900:Anti-Bacterial Agents; D012834:Silver
keywords: Antibiotics; Antimicrobial activity; Shape and size; Silver nanoparticles; Synergistic effect; Trichoderma viride