title: Hybrid Aorta Constructs via In Situ Crosslinking of Poly(glycerol-sebacate) Elastomer Within a Decellularized Matrix.
abstract: Decellularization of tissues and organs has high potential to obtain unique conformation and composition as native tissue structure but may result in weakened tissue mechanical strength. In this study, poly(glycerol-sebacate) (PGS) elastomers were combined with decellularized aorta fragments to investigate the changes in mechanical properties. PGS prepolymer was synthesized via microwave irradiation and then in situ crosslinked within the decellularized aorta extracellular matrix (ECM). Tensile strength (σ) values were found comparable as 0.44 ± 0.10 MPa and 0.57 ± 0.18 MPa for native and hybrid aorta samples, respectively, while elongation at break (ɛ) values were 261% ± 17%, 7.5% ± 0.57%, and 22.18% ± 2.48% for wet control (native), decellularized dried aortae, and hybrid matrices, showing elastic contribution. Young's modulus data indicate that there was a threefold decrease in stiffness compared to decellularized samples once PGS is introduced into the ECM structure. Scanning electron microscopy (SEM) analysis of hybrid grafts revealed that the construct preserves porosity in medial layer of the vessel. Biocompatibility analyses showed no cytotoxic effects on human abdominal aorta smooth muscle cells. Cell studies showed 98% activity in hybrid graft extracts. As a control, collagen coating of the hybrid grafts was performed in the recellularization stage. SEM analysis of recellularized hybrid grafts revealed that cells were attached to the surface of the hybrid graft and proliferated during the 14 days of culture in both groups. This study shows that introducing an elastomer into the native ECM structure following decellularization process can be a useful approach for the preparation of mechanically enhanced composites for soft tissues.
authors.affiliation: 1 Bioengineering Division, Institute of Science, Hacettepe University , Ankara, Turkey .
authors.forename: Selcan
authors.initials: S
authors.lastname: Guler
authors.affiliation: 2 Nanotechnology and Nanomedicine Division, Institute of Science, Hacettepe University , Ankara, Turkey .
authors.forename: Pezhman
authors.initials: P
authors.lastname: Hosseinian
authors.affiliation: 3 Environmental Engineering Department & Bioengineering Division and Centre for Bioengineering, Hacettepe University , Ankara, Turkey .
authors.forename: Halil Murat
authors.initials: HM
authors.lastname: Aydin
mesh_terms: D000818:Animals; D001011:Aorta / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D002478:Cells, Cultured; D003432:Cross-Linking Reagents / Q000378:metabolism*; D003651:Decanoates / Q000737:chemistry*; D020724:Elastomers / Q000737:chemistry*; D005109:Extracellular Matrix / Q000378:metabolism*; D005990:Glycerol / Q000031:analogs & derivatives* / Q000737:chemistry; D006801:Humans; D008422:Materials Testing; D008855:Microscopy, Electron, Scanning; D032389:Myocytes, Smooth Muscle / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D011108:Polymers / Q000737:chemistry*; D016062:Porosity; D012756:Sheep; D013718:Tensile Strength; D023822:Tissue Engineering / Q000379:methods*
chemical_list: D003432:Cross-Linking Reagents; D003651:Decanoates; D020724:Elastomers; D011108:Polymers; C469892:poly(glycerol-sebacate); D005990:Glycerol
keywords: aorta; composite matrices; decellularization; poly(glycerol-sebacate); tissue engineering