title: Insights into the Regulation of Rhizosphere Bacterial Communities by Application of Bio-organic Fertilizer inPseudostellaria heterophyllaMonoculture Regime.
abstract: The biomass and quality ofPseudostellariae heterophyllasuffers a significant decline under monoculture. Since rhizosphere miobiome plays crucial roles in soil health, deep pyrosequencing combined with qPCR was applied to characterize the composition and structure of soil bacterial community under monoculture and different amendments. The results showed compared with the 1st-year planted (FP), 2nd-year monoculture ofP. heterophylla(SP) led to a significant decline in yield and resulted in a significant increase inFusarium oxysporumbut a decline inBurkholderiaspp. Bio-organic fertilizer (MT) formulated by combining antagonistic bacteria with organic matter could significantly promote the yield by regulating rhizosphere bacterial community. However, organic fertilizer (MO) without antagonistic bacteria could not suppressFusariumwilt. Multivariate statistics analysis showed a distinct separation between the healthy samples (FP and MT) and the unhealthy samples (SP and MO), suggesting a strong relationship between soil microbial community and plant performance. Furthermore, we found the application of bio-organic fertilizer MT could significantly increase the bacterial community diversity and restructure microbial community with relatively fewer pathogenicF. oxysporumand more beneficialBurkholderiaspp. In conclusion, the application of novel bio-organic fertilizer could effectively suppressFusariumwilt by enriching the antagonistic bacteria and enhancing the bacterial diversity.
authors.affiliation: College of Life Sciences, Fujian Agriculture and Forestry UniversityFuzhou, China; Key Laboratory of Crop Ecology and Molecular Physiology, Fujian Agriculture and Forestry UniversityFuzhou, China.
authors.forename: Linkun
authors.initials: L
authors.lastname: Wu
authors.affiliation: College of Life Sciences, Fujian Agriculture and Forestry UniversityFuzhou, China; Key Laboratory of Crop Ecology and Molecular Physiology, Fujian Agriculture and Forestry UniversityFuzhou, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Chen
authors.affiliation: College of Life Sciences, Fujian Agriculture and Forestry UniversityFuzhou, China; Key Laboratory of Crop Ecology and Molecular Physiology, Fujian Agriculture and Forestry UniversityFuzhou, China.
authors.forename: Hongmiao
authors.initials: H
authors.lastname: Wu
authors.affiliation: Key Laboratory of Crop Ecology and Molecular Physiology, Fujian Agriculture and Forestry UniversityFuzhou, China; College of Crop Science, Fujian Agriculture and Forestry UniversityFuzhou, China.
authors.forename: Xianjin
authors.initials: X
authors.lastname: Qin
authors.affiliation: College of Life Sciences, Fujian Agriculture and Forestry UniversityFuzhou, China; Key Laboratory of Crop Ecology and Molecular Physiology, Fujian Agriculture and Forestry UniversityFuzhou, China.
authors.forename: Juanying
authors.initials: J
authors.lastname: Wang
authors.affiliation: College of Life Sciences, Fujian Agriculture and Forestry UniversityFuzhou, China; Key Laboratory of Crop Ecology and Molecular Physiology, Fujian Agriculture and Forestry UniversityFuzhou, China.
authors.forename: Yanhong
authors.initials: Y
authors.lastname: Wu
authors.affiliation: College of Life Sciences, Fujian Agriculture and Forestry UniversityFuzhou, China; Key Laboratory of Crop Ecology and Molecular Physiology, Fujian Agriculture and Forestry UniversityFuzhou, China.
authors.forename: Muhammad U
authors.initials: MU
authors.lastname: Khan
authors.affiliation: College of Life Sciences, Fujian Agriculture and Forestry UniversityFuzhou, China; Key Laboratory of Crop Ecology and Molecular Physiology, Fujian Agriculture and Forestry UniversityFuzhou, China.
authors.forename: Sheng
authors.initials: S
authors.lastname: Lin
authors.affiliation: College of Life Sciences, Fujian Agriculture and Forestry UniversityFuzhou, China; Key Laboratory of Crop Ecology and Molecular Physiology, Fujian Agriculture and Forestry UniversityFuzhou, China.
authors.forename: Zhigang
authors.initials: Z
authors.lastname: Xiao
authors.affiliation: College of Crop Science, Fujian Agriculture and Forestry UniversityFuzhou, China; Fujian Provincial Key Laboratory of Agroecological Processing and Safety Monitoring, Fujian Agriculture and Forestry UniversityFuzhou, China.
authors.forename: Xiaomian
authors.initials: X
authors.lastname: Luo
authors.affiliation: College of Crop Science, Fujian Agriculture and Forestry UniversityFuzhou, China; Fujian Provincial Key Laboratory of Agroecological Processing and Safety Monitoring, Fujian Agriculture and Forestry UniversityFuzhou, China.
authors.forename: Zhongyi
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: College of Life Sciences, Fujian Agriculture and Forestry UniversityFuzhou, China; Fujian Provincial Key Laboratory of Agroecological Processing and Safety Monitoring, Fujian Agriculture and Forestry UniversityFuzhou, China.
authors.forename: Wenxiong
authors.initials: W
authors.lastname: Lin
keywords: Pseudostellaria heterophylla; bio-organic fertilizer; deep pyrosequencing; microbial community; replant disease