title: N-acetylglucosamine-Mediated Expression ofnagAandnagBinStreptococcus pneumoniae.
abstract: In this study, we have explored the transcriptomic response ofStreptococcus pneumoniaeD39 to N-acetylglucosamine (NAG). Transcriptome comparison ofS. pneumoniaeD39 wild-type grown in chemically defined medium (CDM) in the presence of 0.5% NAG to that grown in the presence of 0.5% glucose revealed elevated expression of many genes/operons, includingnagA, nagB, manLMN, andnanP. We have further confirmed the NAG-dependent expression ofnagA, nagB, manLMN, andnanPby β-galactosidase assays.nagA, nagBandglmSare putatively regulated by a transcriptional regulator NagR. We predicted the operator site of NagR (dresite) in PnagA, PnagB, and PglmS, which was further confirmed by mutating the predicteddresite in the respective promoters (nagA, nagB, andglmS). Growth comparison of ΔnagA, ΔnagB, and ΔglmSwith the D39 wild-type demonstrates thatnagAandnagBare essential forS. pneumoniaeD39 to grow in the presence of NAG as a sole carbon source. Furthermore, deletion ofccpAshows that CcpA has no effect on the expression ofnagA, nagB, andglmSin the presence of NAG inS.pneumoniae.
authors.affiliation: Department of Molecular Genetics, Groningen Biomolecular Sciences and Biotechnology Institute, University of GroningenGroningen, Netherlands; Department of Bioinformatics and Biotechnology, Government College UniversityFaisalabad, Pakistan.
authors.forename: Muhammad
authors.initials: M
authors.lastname: Afzal
authors.affiliation: Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet Stockholm, Sweden.
authors.forename: Sulman
authors.initials: S
authors.lastname: Shafeeq
authors.affiliation: Department of Molecular Genetics, Groningen Biomolecular Sciences and Biotechnology Institute, University of GroningenGroningen, Netherlands; Department of Bioinformatics and Biotechnology, Government College UniversityFaisalabad, Pakistan.
authors.forename: Irfan
authors.initials: I
authors.lastname: Manzoor
authors.affiliation: Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet Stockholm, Sweden.
authors.forename: Birgitta
authors.initials: B
authors.lastname: Henriques-Normark
authors.affiliation: Department of Molecular Genetics, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen Groningen, Netherlands.
authors.forename: Oscar P
authors.initials: OP
authors.lastname: Kuipers
mesh_terms: D000117:Acetylglucosamine / Q000494:pharmacology*; D000606:Amino Sugars / Q000378:metabolism; D001426:Bacterial Proteins / Q000187:drug effects* / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D002244:Carbon / Q000378:metabolism; D004269:DNA, Bacterial; D004926:Escherichia coli / Q000235:genetics; D029968:Escherichia coli Proteins / Q000235:genetics; D020869:Gene Expression Profiling; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects*; D005798:Genes, Bacterial / Q000235:genetics*; D005947:Glucose / Q000378:metabolism; D011401:Promoter Regions, Genetic / Q000235:genetics; D017384:Sequence Deletion; D013296:Streptococcus pneumoniae / Q000187:drug effects* / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D001616:beta-Galactosidase / Q000032:analysis; D005945:Glutamine-Fructose-6-Phosphate Transaminase (Isomerizing)
chemical_list: D000117:Acetylglucosamine; D000606:Amino Sugars; D001426:Bacterial Proteins; D002244:Carbon; D004269:DNA, Bacterial; D029968:Escherichia coli Proteins; D005947:Glucose; D001616:beta-Galactosidase; C000731191:component S, glutamate mutase protein, Bacteria; D005945:Glutamine-Fructose-6-Phosphate Transaminase (Isomerizing)
keywords: CcpA; GlmS; N-acetylglucosamine; NAG; NagA; NagB; pneumococcus