title: Construction of a Miniaturized Chromatic Acclimation Sensor from Cyanobacteria with Reversed Response to a Light Signal.
abstract: Cyanobacteria harbor unique photoreceptors, designated as cyanobacteriochromes (CBCRs). In this study, we attempted to engineer the chromatic acclimation sensor CcaS, a CBCR derived from the cyanobacterium Synechocystis sp. PCC 6803. The wild-type CcaS induces gene expression under green light illumination and represses it under red light illumination. We focused on the domain structure of CcaS, which consists of an N-terminal transmembrane helix; a GAF domain, which serves as the sensor domain; a linker region (L1); two PAS domains; a second linker region (L2); and a C-terminal histidine kinase (HK) domain. Truncated versions of the photoreceptor were constructed by removing the L1 linker region and the two PAS domains, and fusing the GAF and HK domains with a truncated linker region. Thus constructed "miniaturized CcaSs" were grouped into four distinct categories according to their responses toward green and red light illumination, with some showing improved gene regulation compared to the wild type. Remarkably, one of the miniaturized CcaSs induced gene expression under red light and repressed it under green light, a reversed response to the light signal compared to wild type CcaS. These characteristics of engineered photoreceptors were discussed by analyzing the CcaS structural model.
authors.affiliation: Department of Biotechnology and Life Science, Graduate School of Engineering, Tokyo University of Agriculture &Technology, 2-24-16 Naka-cho, Koganei, Tokyo 184-8588, Japan.|JST, CREST, 2-24-16 Naka-cho, Koganei, Tokyo, 184-8588, Japan.
authors.forename: Mitsuharu
authors.initials: M
authors.lastname: Nakajima
authors.affiliation: JST, CREST, 2-24-16 Naka-cho, Koganei, Tokyo, 184-8588, Japan.|Department of Applied Chemistry and Biochemical Engineering, Shizuoka University, 3-5-1 Johoku, Naka-ku, Hamamatsu, Shizuoka, 432-8561, Japan.
authors.forename: Stefano
authors.initials: S
authors.lastname: Ferri
authors.affiliation: Plant Biochemistry, Faculty of Biology &Biotechnology, Ruhr-Universität Bochum, Universitätsstr. 150, 44780, Bochum, Germany.
authors.forename: Matthias
authors.initials: M
authors.lastname: Rögner
authors.affiliation: Department of Biotechnology and Life Science, Graduate School of Engineering, Tokyo University of Agriculture &Technology, 2-24-16 Naka-cho, Koganei, Tokyo 184-8588, Japan.|JST, CREST, 2-24-16 Naka-cho, Koganei, Tokyo, 184-8588, Japan.|Institute of Global Research Innovation, Tokyo University of Agriculture &Technology, 2-24-16 Naka-cho, Koganei, Tokyo 184-8588, Japan.
authors.forename: Koji
authors.initials: K
authors.lastname: Sode
mesh_terms: D000064:Acclimatization; D001426:Bacterial Proteins / Q000737:chemistry / Q000378:metabolism; D002478:Cells, Cultured; D000458:Cyanobacteria / Q000378:metabolism*; D005453:Fluorescence; D015964:Gene Expression Regulation, Bacterial; D055537:Light Signal Transduction; D008904:Miniaturization / Q000295:instrumentation*; D008958:Models, Molecular; D000072417:Protein Domains
chemical_list: D001426:Bacterial Proteins