title: Simultaneous Detection of Three Bacterial Seed-Borne Diseases in Rice Using Multiplex Polymerase Chain Reaction.
abstract: Burkholderia glumae(bacterial grain rot),Xanthomonas oryzaepv.oryzae(bacterial leaf blight), andAcidovorax avenaesubsp.avenae(bacterial brown stripe) are major seedborne pathogens of rice. Based on the 16S and 23S rDNA sequences forA. avenaesubsp.avenaeandB. glumae, and transposase A gene sequence forX. oryzaepv.oryzae, three sets of primers had been designed to produce 402 bp forB. glumae, 490 bp forX. oryzae, and 290 bp forA. avenaesubsp.avenaewith the 63°C as an optimum annealing temperature. Samples collected from naturally infected fields were detected with two bacteria,B. glumaeandA. avenaesubsp.avenaebutX. oryzaepv.oryzaewas not detected. This assay can be used to identify pathogens directly from infected seeds, and will be an effective tool for the identification of the three pathogens in rice plants.
authors.affiliation: Crop Cultivation and Environment Research Division, National Institute of Crop Science, Rural Development Administration, Suwon 16429, Korea.
authors.forename: In Jeong
authors.initials: IJ
authors.lastname: Kang
authors.affiliation: Capacity Building Division, Rural Development Administration, Jeonju 54875, Korea.
authors.forename: Mi-Hyung
authors.initials: MH
authors.lastname: Kang
authors.affiliation: Planning and Coordination Division, National Institute of Crop Science, Rural Development Administration, Jeonju 55365, Korea.
authors.forename: Tae-Hwan
authors.initials: TH
authors.lastname: Noh
authors.affiliation: Crop Cultivation and Environment Research Division, National Institute of Crop Science, Rural Development Administration, Suwon 16429, Korea.
authors.forename: Hyeong Kwon
authors.initials: HK
authors.lastname: Shim
authors.affiliation: Crop Cultivation and Environment Research Division, National Institute of Crop Science, Rural Development Administration, Suwon 16429, Korea.
authors.forename: Dong Bum
authors.initials: DB
authors.lastname: Shin
authors.affiliation: Crop Cultivation and Environment Research Division, National Institute of Crop Science, Rural Development Administration, Suwon 16429, Korea.
authors.forename: Suggi
authors.initials: S
authors.lastname: Heu
keywords: multiplex PCR; rice bacterial pathogens; simultaneous detection