title: Maripseudobacter aurantiacus gen. nov., sp. nov., a novel member of the family Flavobacteriaceae isolated from a sedimentation basin.
abstract: A Gram-stain negative, orange-pigmented, aerobic, non-motile and ovoid- to rod-shaped bacterial strain, designated CDA4T, was isolated from a sediment sample collected from the sedimentation basin of a mariculture farm in Zhejiang province, China. The temperature range for growth of strain CDA4T was 15-40 °C, wirrth an optimum at 35 °C. The pH range for growth was 6.0-8.5, with an optimum around pH 7.5. NaCl was required for growth at the concentration range 0.5-5.0 % (w/v), with an optimum at 2.0 % (w/v). Chemotaxonomic analysis indicated that the main respiratory quinone was menaquinone 6 (MK-6), the predominant cellular fatty acids were iso-C15 : 0, iso-C15 : 1 G, unknown ECL 13.565 and iso-C17 : 0 3-OH, and the major polar lipids were phosphatidylethanolamine, two unidentified lipids, two unidentified phospholipids and four unidentified aminolipids. The genomic DNA G+C content of strain CDA4T was 38.3 mol%. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain CDA4T formed a distinct lineage in the clade of the family Flavobacteriaceae. Based on the polyphasic taxonomic characterization, strain CDA4T is considered to represent a novel species of a new genus, for which the name Maripseudobacter aurantiacus gen. nov., sp. nov. is proposed. The type strain of the type species is CDA4T (=KCTC 52409T=MCCC 1K03210T).
authors.affiliation: Ocean College, Zhejiang University, Hangzhou 310058, PR China.
authors.forename: Can
authors.initials: C
authors.lastname: Chen
authors.affiliation: Ocean College, Zhejiang University, Hangzhou 310058, PR China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Su
authors.affiliation: College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, PR China.
authors.forename: Tianyi
authors.initials: T
authors.lastname: Tao
authors.affiliation: Ocean College, Zhejiang University, Hangzhou 310058, PR China.
authors.forename: Geyi
authors.initials: G
authors.lastname: Fu
authors.affiliation: Ocean College, Zhejiang University, Hangzhou 310058, PR China.
authors.forename: Chongya
authors.initials: C
authors.lastname: Zhang
authors.affiliation: College of Life Sciences, Zhejiang Sci-Tech University, Hangzhou 310018, PR China.
authors.forename: Cong
authors.initials: C
authors.lastname: Sun
authors.affiliation: School of Forestry and Biotechnology, Zhejiang Agriculture and Forestry University, Lin'an 311300, PR China.
authors.forename: Xinqi
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Ocean College, Zhejiang University, Hangzhou 310058, PR China.
authors.forename: Min
authors.initials: M
authors.lastname: Wu
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D002681:China; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D041965:Flavobacteriaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D019015:Geologic Sediments / Q000382:microbiology*; D010743:Phospholipids / Q000737:chemistry; D010802:Phylogeny; D010858:Pigmentation; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012623:Seawater / Q000737:chemistry*; D017422:Sequence Analysis, DNA; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; C042455:menaquinone 6