title: [Vitamin D metabolism and signaling in human hepatocellular carcinoma and surrounding non-tumorous liver].
abstract: BACKGROUND
  1,25-Dihydroxy vitamin D3mediates antitumor effects in hepatocellular carcinoma.
  
  
  OBJECTIVE
  We examined mRNA and protein expression differences in 1,25-Dihydroxy vitamin D3-inactivating CYP24A1, mRNA of activating CYP27B1 enzymes, and that of VDR between human hepatocellular carcinoma and surrounding non-tumorous liver.
  
  
  METHODS
  Snap-frozen tissues from 13 patients were studied for mRNA and protein expression of CYP24A1. Paraffin-embedded tissues from 36 patients were used to study mRNA of VDR and CYP27B1. mRNA expression was measured by RT-PCR, CYP24A1 protein was detected by immunohistochemistry.
  
  
  RESULTS
  Expression of VDR and CYP27B1 was significantly lower in hepatocellular carcinoma compared with non-tumorous liver (p<0.05). The majority of the HCC samples expressed CYP24A1 mRNA, but neither of the non-tumorous liver. The gene activation was followed by CYP24A1 protein synthesis.
  
  
  CONCLUSIONS
  The presence of CYP24A1 mRNA and the reduced expression of VDR and CYP27B1 mRNA in human hepatocellular carcinoma samples indicate decreased bioavailability of 1,25-Dihydroxy vitamin D3, providing an escape mechanism from the anti-tumor effect. Orv. Hetil., 2016, 157(48), 1910-1918.
authors.affiliation: I. Belgyógyászati Klinika, Semmelweis Egyetem, Általános Orvostudományi Kar Budapest, Korányi Sándor u. 2/A, 1083.
authors.forename: Evelin
authors.initials: E
authors.lastname: Horváth
authors.affiliation: I. Belgyógyászati Klinika, Semmelweis Egyetem, Általános Orvostudományi Kar Budapest, Korányi Sándor u. 2/A, 1083.
authors.forename: Bernadett
authors.initials: B
authors.lastname: Balla
authors.affiliation: I. Belgyógyászati Klinika, Semmelweis Egyetem, Általános Orvostudományi Kar Budapest, Korányi Sándor u. 2/A, 1083.
authors.forename: János
authors.initials: J
authors.lastname: Kósa
authors.affiliation: I. Belgyógyászati Klinika, Semmelweis Egyetem, Általános Orvostudományi Kar Budapest, Korányi Sándor u. 2/A, 1083.
authors.forename: Péter András
authors.initials: PA
authors.lastname: Lakatos
authors.affiliation: Országos Gerincgyógyászati Központ Budapest.
authors.forename: Áron
authors.initials: Á
authors.lastname: Lazáry
authors.affiliation: I. Belgyógyászati Klinika, Semmelweis Egyetem, Általános Orvostudományi Kar Budapest, Korányi Sándor u. 2/A, 1083.
authors.forename: Dániel
authors.initials: D
authors.lastname: Németh
authors.affiliation: I. Belgyógyászati Klinika, Semmelweis Egyetem, Általános Orvostudományi Kar Budapest, Korányi Sándor u. 2/A, 1083.
authors.forename: Hasan
authors.initials: H
authors.lastname: Jozilan
authors.affiliation: II. Patológiai Intézet, Semmelweis Egyetem, Általános Orvostudományi Kar Budapest.
authors.forename: Áron
authors.initials: Á
authors.lastname: Somorácz
authors.affiliation: II. Patológiai Intézet, Semmelweis Egyetem, Általános Orvostudományi Kar Budapest.
authors.forename: Anna
authors.initials: A
authors.lastname: Korompay
authors.affiliation: II. Patológiai Intézet, Semmelweis Egyetem, Általános Orvostudományi Kar Budapest.
authors.forename: Benedek
authors.initials: B
authors.lastname: Gyöngyösi
authors.affiliation: II. Patológiai Intézet, Semmelweis Egyetem, Általános Orvostudományi Kar Budapest.
authors.forename: Katalin
authors.initials: K
authors.lastname: Borka
authors.affiliation: II. Patológiai Intézet, Semmelweis Egyetem, Általános Orvostudományi Kar Budapest.
authors.forename: András
authors.initials: A
authors.lastname: Kiss
authors.affiliation: I. Sebészeti Klinika, Semmelweis Egyetem, Általános Orvostudományi Kar Budapest.
authors.forename: Péter
authors.initials: P
authors.lastname: Kupcsulik
authors.affiliation: II. Patológiai Intézet, Semmelweis Egyetem, Általános Orvostudományi Kar Budapest.
authors.forename: Zsuzsa
authors.initials: Z
authors.lastname: Schaff
authors.affiliation: I. Belgyógyászati Klinika, Semmelweis Egyetem, Általános Orvostudományi Kar Budapest, Korányi Sándor u. 2/A, 1083.
authors.forename: Ferenc
authors.initials: F
authors.lastname: Szalay
mesh_terms: D015090:25-Hydroxyvitamin D3 1-alpha-Hydroxylase / Q000235:genetics / Q000378:metabolism*; D006528:Carcinoma, Hepatocellular / Q000378:metabolism*; D053493:Cholestanetriol 26-Monooxygenase / Q000378:metabolism; D000072467:Cytochrome P450 Family 2 / Q000378:metabolism; D015870:Gene Expression / Q000187:drug effects*; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism*; D065668:Vitamin D3 24-Hydroxylase / Q000378:metabolism
chemical_list: D012333:RNA, Messenger; D000072467:Cytochrome P450 Family 2; C506833:CYP2R1 protein, human; D053493:Cholestanetriol 26-Monooxygenase; C585549:CYP24A1 protein, human; D065668:Vitamin D3 24-Hydroxylase; D015090:25-Hydroxyvitamin D3 1-alpha-Hydroxylase
keywords: CYP24A1; CYP27B1; D-vitamin; hepatocellular carcinoma; hepatocellularis carcinoma; vitamin D