title: Atmospheric pressure thermal ionization ion source for peptide analysis.
abstract: A novel atmospheric pressure thermal ionization (APTI) ion source was developed for the analysis of liquid samples. The feasibility of the ion source was demonstrated on peptides using two configurations-assisted by hot wire or hot surface. Microalloyed molybdenum, used as a thermal ion- emitter, notably facilitated the formation of multiply-charged ions, but fragmentation products were still observed. Peptide fragmentation accompanying thermal ionization can be used for peptide identification. The described method is promising for studies of biological samples with minimal pre-treatment.
authors.affiliation: Moscow Institute of Physics and Technology, 141700 Dolgoprudny, Moscow Region, Russian Federation and Institute for Energy Problems of Chemical Physics, Russian Academy of Sciences, 119334 Moscow, Russian Federation.
authors.forename: Eugenii N
authors.initials: EN
authors.lastname: Kukaev
authors.affiliation: Moscow Institute of Physics and Technology, 141700 Dolgoprudny, Moscow Region, Russian Federation and V.I. Kulakov Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Healthcare of the Russian Federation, Moscow, Russia.
authors.forename: Alexey S
authors.initials: AS
authors.lastname: Kononikhin
authors.affiliation: Institute for Energy Problems of Chemical Physics, Russian Academy of Sciences, 119334 Moscow, Russian Federation and V.I. Kulakov Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Healthcare of the Russian Federation, Moscow, Russia.
authors.forename: Natalia L
authors.initials: NL
authors.lastname: Starodubtseva
authors.affiliation: Moscow Institute of Physics and Technology, 141700 Dolgoprudny, Moscow Region, Russian Federation and Institute for Energy Problems of Chemical Physics, Russian Academy of Sciences, 119334 Moscow, Russian Federation and Skolkovo Institute of Science and Technology, 143025 Skolkovo, Moscow region, Russian Federation.
authors.forename: Yury I
authors.initials: YI
authors.lastname: Kostyukevich
authors.affiliation: Moscow Institute of Physics and Technology, 141700 Dolgoprudny, Moscow Region, Russian Federation and Institute for Energy Problems of Chemical Physics, Russian Academy of Sciences, 119334 Moscow, Russian Federation and V.I. Kulakov Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Healthcare of the Russian Federation, Moscow, Russia.
authors.forename: Igor A
authors.initials: IA
authors.lastname: Popova
authors.affiliation: V.I. Kulakov Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Healthcare of the Russian Federation, Moscow, Russia.
authors.forename: Vitaliy
authors.initials: V
authors.lastname: Chagovets
authors.affiliation: Institute for Energy Problems of Chemical Physics, Russian Academy of Sciences, 119334 Moscow, Russian Federation.
authors.forename: Konstantin O
authors.initials: KO
authors.lastname: Nagornov
authors.affiliation: Moscow Institute of Physics and Technology, 141700 Dolgoprudny, Moscow Region, Russian Federation and Institute for Energy Problems of Chemical Physics, Russian Academy of Sciences, 119334 Moscow, Russian Federation and Skolkovo Institute of Science and Technology, 143025 Skolkovo, Moscow region, Russian Federation.
authors.forename: Eugene N
authors.initials: EN
authors.lastname: Nikolaev
mesh_terms: D001274:Atmospheric Pressure; D004867:Equipment Design; D019544:Equipment Failure Analysis; D006361:Heating / Q000295:instrumentation*; D010449:Peptide Mapping / Q000379:methods*; D010455:Peptides / Q000032:analysis* / Q000737:chemistry*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D021241:Spectrometry, Mass, Electrospray Ionization / Q000295:instrumentation*
chemical_list: D010455:Peptides