title: The effects of zofenopril on cardiac function and pro-oxidative parameters in the streptozotocin-induced diabetic rat heart.
abstract: Diabetes mellitus is a chronic condition that continues to increase in both incidence and prevalence. Renin-Angiotensin-Aldosterone System is one of the main modulators of chronic hyperglycaemia and, thus, its influence on tissues. Hyperglycaemia-induced oxidative stress is an important factor in diabetic cardiomyopathy. The present study was carried out on 24 adult male Wistar albino rats (8-week-old and with body masses of 190 ± 10 g). We evaluated the influence of acute administration of zofenopril on ex vivo myocardial function from rats with streptozotocin-induced diabetes mellitus, with a special emphasis on cardiodynamic and oxidative stress parameters in diabetic rat hearts. Rats were divided randomly into two groups (12 animals per group): control non-diabetic animals (C) were healthy rats perfused with 1.5 µM of zofenopril, and STZ-treated diabetic animals (DM) were diabetic animals perfused with 1.5 µM of zofenopril 4 weeks after the induction of diabetes. Our results demonstrated that diabetic rats are characterized by a depressed cardiac performance and that oxidative markers are related to alterations in cardiac function in rats with 4 weeks of STZ-induced diabetes. Additionally, the use of zofenopril as a monotherapy slightly diminished cardiac damage induced by chronic hyperglycaemia. However, long-term follow-up intervention trials are necessary to fully demonstrate the benefit of zofenopril in this context. A challenge for future investigations will be to identify the effects of chronic administration or combination therapy with angiotensin-converting enzyme inhibitors in various models of diabetes.
authors.affiliation: Clinic of Endocrinology, Military Medical Academy, Belgrade, Serbia.
authors.forename: Petar
authors.initials: P
authors.lastname: Ristic
authors.affiliation: Department of Physiology, Faculty of Medical Sciences, University of Kragujevac, Svetozara Markovica 69, 34000, Kragujevac, Serbia.
authors.forename: Ivan
authors.initials: I
authors.lastname: Srejovic
authors.affiliation: Department of Pharmacy, Faculty of Medical Sciences, University of Kragujevac, Kragujevac, Serbia.
authors.forename: Tamara
authors.initials: T
authors.lastname: Nikolic
authors.affiliation: Department of Pharmacy, Faculty of Medical Sciences, University of Kragujevac, Kragujevac, Serbia.
authors.forename: Isidora
authors.initials: I
authors.lastname: Stojic
authors.affiliation: Clinic of Ophthalmology, Military Medical Academy, Belgrade, Serbia.
authors.forename: Dragana
authors.initials: D
authors.lastname: Ristic
authors.affiliation: Department of Physiology, Faculty of Medical Sciences, University of Kragujevac, Svetozara Markovica 69, 34000, Kragujevac, Serbia.
authors.forename: Vladimir
authors.initials: V
authors.lastname: Zivkovic
authors.affiliation: Department of Physiology, Faculty of Medical Sciences, University of Kragujevac, Svetozara Markovica 69, 34000, Kragujevac, Serbia. drvladakgbg@yahoo.com.
authors.forename: Vladimir Lj
authors.initials: VL
authors.lastname: Jakovljevic
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000097:blood; D002216:Captopril / Q000031:analogs & derivatives* / Q000494:pharmacology; D003921:Diabetes Mellitus, Experimental / Q000097:blood / Q000188:drug therapy* / Q000503:physiopathology*; D058065:Diabetic Cardiomyopathies / Q000097:blood / Q000188:drug therapy* / Q000503:physiopathology*; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D015415:Biomarkers; C044958:zofenopril; D002216:Captopril
keywords: Cardiodynamics; Isolated heart; Oxidative parameter; Rat; Streptozotocin