title: Impact of external pneumatic compression target inflation pressure on transcriptome-wide RNA expression in skeletal muscle.
abstract: Next-generation RNA sequencing was employed to determine the acute and subchronic impact of peristaltic pulse external pneumatic compression (PEPC) of different target inflation pressures on global gene expression in human vastus lateralis skeletal muscle biopsy samples. Eighteen (N = 18) male participants were randomly assigned to one of the three groups: (1) sham (n = 6), 2) EPC at 30-40 mmHg (LP-EPC; n = 6), and 3) EPC at 70-80 mmHg (MP-EPC; n = 6). One hour treatment with sham/EPC occurred for seven consecutive days. Vastus lateralis skeletal muscle biopsies were performed at baseline (before first treatment; PRE), 1 h following the first treatment (POST1), and 24 h following the last (7th) treatment (POST2). Changes from PRE in gene expression were analyzed via paired comparisons within each group. Genes were filtered to include only those that had an RPKM ≥ 1.0, a fold-change of ≥1.5 and a paired t-test value of <0.01. For the sham condition, two genes at POST1 and one gene at POST2 were significantly altered. For the LP-EPC condition, nine genes were up-regulated and 0 genes were down-regulated at POST1 while 39 genes were up-regulated and one gene down-regulated at POST2. For the MP-EPC condition, two genes were significantly up-regulated and 21 genes were down-regulated at POST1 and 0 genes were altered at POST2. Both LP-EPC and MP-EPC acutely alter skeletal muscle gene expression, though only LP-EPC appeared to affect gene expression with subchronic application. Moreover, the transcriptome response to EPC demonstrated marked heterogeneity (i.e., genes and directionality) with different target inflation pressures.
authors.affiliation: Department of Cell Biology and Physiology, Edward Via College of Osteopathic Medicine - Auburn Campus, Auburn, Alabama jmartin@auburn.vcom.edu.|School of Kinesiology, Auburn University, Auburn, Alabama.
authors.forename: Jeffrey S
authors.initials: JS
authors.lastname: Martin
authors.affiliation: School of Kinesiology, Auburn University, Auburn, Alabama.
authors.forename: Wesley C
authors.initials: WC
authors.lastname: Kephart
authors.affiliation: School of Kinesiology, Auburn University, Auburn, Alabama.
authors.forename: Cody T
authors.initials: CT
authors.lastname: Haun
authors.affiliation: School of Kinesiology, Auburn University, Auburn, Alabama.
authors.forename: Anna E
authors.initials: AE
authors.lastname: McCloskey
authors.affiliation: School of Kinesiology, Auburn University, Auburn, Alabama.
authors.forename: Joshua J
authors.initials: JJ
authors.lastname: Shake
authors.affiliation: School of Kinesiology, Auburn University, Auburn, Alabama.
authors.forename: Christopher B
authors.initials: CB
authors.lastname: Mobley
authors.affiliation: Department of Cell Biology and Physiology, Edward Via College of Osteopathic Medicine - Auburn Campus, Auburn, Alabama.|Athletics Department, Auburn University, Auburn, Alabama.
authors.forename: Michael D
authors.initials: MD
authors.lastname: Goodlett
authors.affiliation: Department of Cell Biology and Physiology, Edward Via College of Osteopathic Medicine - Auburn Campus, Auburn, Alabama.|School of Kinesiology, Auburn University, Auburn, Alabama.
authors.forename: Andreas
authors.initials: A
authors.lastname: Kavazis
authors.affiliation: School of Kinesiology, Auburn University, Auburn, Alabama.
authors.forename: David D
authors.initials: DD
authors.lastname: Pascoe
authors.affiliation: Department of Entomology and Plant Pathology, Auburn University, Auburn, Alabama.
authors.forename: Lee
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Department of Cell Biology and Physiology, Edward Via College of Osteopathic Medicine - Auburn Campus, Auburn, Alabama.|School of Kinesiology, Auburn University, Auburn, Alabama.
authors.forename: Michael D
authors.initials: MD
authors.lastname: Roberts
mesh_terms: D000328:Adult; D001706:Biopsy / Q000379:methods; D002950:Citrate (si)-Synthase / Q000378:metabolism; D006801:Humans; D048228:Intermittent Pneumatic Compression Devices / Q000009:adverse effects*; D008297:Male; D018482:Muscle, Skeletal / Q000098:blood supply / Q000378:metabolism* / Q000473:pathology; D010084:Oxidation-Reduction; D010528:Peristalsis / Q000502:physiology; D000071248:Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha / Q000378:metabolism; D011312:Pressure; D012313:RNA / Q000235:genetics*; D015533:Transcriptional Activation; D059467:Transcriptome / Q000235:genetics*; D015854:Up-Regulation / Q000235:genetics; D055815:Young Adult
chemical_list: C486497:PPARGC1A protein, human; D000071248:Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha; D012313:RNA; D002950:Citrate (si)-Synthase
keywords: External pneumatic compression; PGC‐1α; RNA sequencing; redox balance; skeletal muscle