title: Simultaneous determination of pyrifluquinazon and its main metabolite in fruits and vegetables by using QuEChERS-HPLC-MS/MS.
abstract: A rapid, reliable, and sensitive method is reported for the simultaneous analysis of pyrifluquinazon and its main metabolite NNI-0101-1H in fruits (strawberry and cherry) and vegetables (cucumber and tomato) using high-performance liquid chromatography coupled with tandem mass spectrometry. A modified, quick, easy, cheap, effective, rugged, and safe procedure was used for the sample pre-preparation. The target analytes were extracted with acetonitrile and then cleaned up using dispersive solid-phase extraction procedure with primary secondary amine. Sample analysis was performed using electrospray ionization in positive mode. Good linearities with the correlation coefficients higher than 0.9991 were obtained in the range of 1-1000 μg/L under the optimized conditions. The average recoveries of the pyrifluquinazon and NNI-0101-1H were in the range of 71.4-106.0% with the relative standard deviations 1.8-11.8% in all matrices at three spiked levels (10, 100, and 1000 μg/kg). The limit of quantification 10 μg/kg was set as the lowest spiked level. The developed method is reliable and effective for the routine monitoring of pyrifluquinazon and its metabolite NNI-0101-1H in fruits and vegetables to ensure food safety.
authors.affiliation: Key Laboratory of Tobacco Pest Monitoring Controlling & Integrated Management, Tobacco Research Institute, Chinese Academy of Agricultural Sciences, Qingdao, Shandong Province, China.
authors.forename: Xiangwei
authors.initials: X
authors.lastname: You
authors.affiliation: Key Laboratory of Tobacco Pest Monitoring Controlling & Integrated Management, Tobacco Research Institute, Chinese Academy of Agricultural Sciences, Qingdao, Shandong Province, China.
authors.forename: Chengcheng
authors.initials: C
authors.lastname: Sui
authors.affiliation: Key Laboratory of Tobacco Pest Monitoring Controlling & Integrated Management, Tobacco Research Institute, Chinese Academy of Agricultural Sciences, Qingdao, Shandong Province, China.
authors.forename: Yiqiang
authors.initials: Y
authors.lastname: Li
authors.affiliation: Key Laboratory of Tobacco Pest Monitoring Controlling & Integrated Management, Tobacco Research Institute, Chinese Academy of Agricultural Sciences, Qingdao, Shandong Province, China.
authors.forename: Xiuguo
authors.initials: X
authors.lastname: Wang
mesh_terms: D002851:Chromatography, High Pressure Liquid; D005504:Food Analysis / Q000379:methods*; D005638:Fruit / Q000737:chemistry*; D057230:Limit of Detection; D010573:Pesticide Residues / Q000032:analysis; D052999:Quinazolinones / Q000032:analysis* / Q000302:isolation & purification; D015203:Reproducibility of Results; D053719:Tandem Mass Spectrometry; D014675:Vegetables / Q000737:chemistry*; D005466:Fluorocarbons
chemical_list: D010573:Pesticide Residues; D052999:Quinazolinones; C581681:1,1,3,4-tetrahydro-6-(1,2,2,2-tetrafluoro-1-(trifluoromethyl)ethyl)quinazolin-2,4-dione; D005466:Fluorocarbons
keywords: high-performance liquid chromatography; insecticides; metabolites; pyrifluquinazon; tandem mass spectrometry