title: Kuwanon G Preserves LPS-Induced Disruption of Gut Epithelial Barrier In Vitro.
abstract: Defects in the gut epithelial barrier have now been recognized to be responsible for diabetic endotoxemia. In everyday life, Mulberry leaf tea is widely used in Asian nations due to its proposed benefits to health and control of diabetes. Evidence indicates the potential role of Kuwanon G (KWG), a component fromMorus albaL., on blocking the gut epithelial barrier. In lipopolysaccharides (LPS)-damaged Caco-2 cells, it was found that KWG increased the viability of cells in a concentration-dependent manner. KWG administration significantly elevated the anti-oxidant abilities via increasing ratio of superoxidase dismutase (SOD)/malondialdehyde (MDA) and decreasing reactive oxygen species (ROS) within the cells. During KWG incubation, pro-inflammatory cytokines including interleukin (IL)-1β and tumor necrosis factor (TNF)-α were significantly reduced, tight junction proteins including zonula occludens (ZO)-1, intercellular adhesion molecule (ICAM)-1 and Occludin were dramatically increased as detected by immunofluorescence assay, trans-epithelial electrical resistance was significantly increased and the transmission of albumin-fluorescein isothiocyanate (FITC) across the barrier was decreased. In conclusion, the present study demonstrated that KWG could ameliorate LPS-induced disruption of the gut epithelial barrier by increasing cell viability and tight junction between cells, and decreasing pro-inflammatory cytokines and oxidative damage.
authors.affiliation: Faculty of Chinese Medicine, Macau University of Science and Technology, Avenida Wai Long, Taipa, Macao, China. henry162037601@gmail.com.
authors.forename: Hengli
authors.initials: H
authors.lastname: Guo
authors.affiliation: Faculty of Chinese Medicine, Macau University of Science and Technology, Avenida Wai Long, Taipa, Macao, China. yhxu@must.edu.mo.|State Key Laboratory of Quality Research in Chinese Medicine, Macau University of Science and Technology, Avenida Wai Long, Taipa, Macao, China. yhxu@must.edu.mo.
authors.forename: Youhua
authors.initials: Y
authors.lastname: Xu
authors.affiliation: Faculty of Chinese Medicine, Macau University of Science and Technology, Avenida Wai Long, Taipa, Macao, China. huangwei1212520@163.com.|State Key Laboratory of Quality Research in Chinese Medicine, Macau University of Science and Technology, Avenida Wai Long, Taipa, Macao, China. huangwei1212520@163.com.|Affiliated Hospital of Southwest Medical University, Luzhou 640000, China. huangwei1212520@163.com.
authors.forename: Wei
authors.initials: W
authors.lastname: Huang
authors.affiliation: Faculty of Chinese Medicine, Macau University of Science and Technology, Avenida Wai Long, Taipa, Macao, China. hzhou@must.edu.mo.|State Key Laboratory of Quality Research in Chinese Medicine, Macau University of Science and Technology, Avenida Wai Long, Taipa, Macao, China. hzhou@must.edu.mo.|Macau Institute for Applied Research in Medicine and Health, Avenida Wai Long, Taipa, Macao, China. hzhou@must.edu.mo.
authors.forename: Hua
authors.initials: H
authors.lastname: Zhou
authors.affiliation: Institute of Consun Co. for Chinese Medicine in Kidney Diseases, Guangdong Consun Pharmaceutical Group, Dongpeng Road 71, Guangzhou 510760, China. dzhg168@126.com.
authors.forename: Zhaoguang
authors.initials: Z
authors.lastname: Zheng
authors.affiliation: Faculty of Chinese Medicine, Macau University of Science and Technology, Avenida Wai Long, Taipa, Macao, China. yhzhao@must.edu.mo.|State Key Laboratory of Quality Research in Chinese Medicine, Macau University of Science and Technology, Avenida Wai Long, Taipa, Macao, China. yhzhao@must.edu.mo.
authors.forename: Yonghua
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: Institute of Consun Co. for Chinese Medicine in Kidney Diseases, Guangdong Consun Pharmaceutical Group, Dongpeng Road 71, Guangzhou 510760, China. hb_consun@163.com.
authors.forename: Bao
authors.initials: B
authors.lastname: He
authors.affiliation: Faculty of Chinese Medicine, Macau University of Science and Technology, Avenida Wai Long, Taipa, Macao, China. zhulinke2012@163.com.|State Key Laboratory of Quality Research in Chinese Medicine, Macau University of Science and Technology, Avenida Wai Long, Taipa, Macao, China. zhulinke2012@163.com.|Affiliated Hospital of Southwest Medical University, Luzhou 640000, China. zhulinke2012@163.com.
authors.forename: Tingting
authors.initials: T
authors.lastname: Zhu
authors.affiliation: Faculty of Chinese Medicine, Macau University of Science and Technology, Avenida Wai Long, Taipa, Macao, China. luckyshaniaya@vip.sina.com.
authors.forename: Shanshan
authors.initials: S
authors.lastname: Tang
authors.affiliation: Faculty of Chinese Medicine, Macau University of Science and Technology, Avenida Wai Long, Taipa, Macao, China. 13570056996@163.com.|State Key Laboratory of Quality Research in Chinese Medicine, Macau University of Science and Technology, Avenida Wai Long, Taipa, Macao, China. 13570056996@163.com.|Institute of Consun Co. for Chinese Medicine in Kidney Diseases, Guangdong Consun Pharmaceutical Group, Dongpeng Road 71, Guangzhou 510760, China. 13570056996@163.com.
authors.forename: Quan
authors.initials: Q
authors.lastname: Zhu
mesh_terms: D018938:Caco-2 Cells; D002470:Cell Survival / Q000187:drug effects; D016207:Cytokines / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D004847:Epithelial Cells / Q000166:cytology / Q000187:drug effects*; D005419:Flavonoids / Q000494:pharmacology*; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D007413:Intestinal Mucosa / Q000166:cytology* / Q000187:drug effects; D008070:Lipopolysaccharides / Q000009:adverse effects*; D008315:Malondialdehyde / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism; D013482:Superoxide Dismutase / Q000378:metabolism; D062725:Tight Junction Proteins / Q000378:metabolism; D019108:Tight Junctions / Q000187:drug effects*
chemical_list: D016207:Cytokines; D005419:Flavonoids; D008070:Lipopolysaccharides; D017382:Reactive Oxygen Species; D062725:Tight Junction Proteins; D008315:Malondialdehyde; C095194:kuwanon G; D013482:Superoxide Dismutase
keywords: Kuwanon G; Morus alba L.; diabetes mellitus; gut epithelial barrier; inflammation