title: Pre-degenerated peripheral nerves co-cultured with bone marrow-derived cells: a new technique for harvesting high-purity Schwann cells.
abstract: Schwann cells play an important role in the peripheral nervous system, especially in nerve repair following injury, so artificial nerve regeneration requires an effective technique for obtaining purified Schwann cells.In vivoandin vitropre-degeneration of peripheral nerves have been shown to obtain high-purity Schwann cells. We believed thatin vitropre-degeneration was simple and controllable, and available for the clinic. Thus, we co-cultured the crushed sciatic nerves with bone marrow-derived cellsin vitro. Results demonstrated that, 3 hours after injury, a large number of mononuclear cells moved to the crushed nerves and a large number of bone marrow-derived cells infiltrated the nerve segments. These changes promoted the degradation of the nerve segments, and the dedifferentiation and proliferation of Schwann cells. Neural cell adhesion molecule and glial fibrillary acidic protein expression were detected in the crushed nerves. Schwann cell yield was 9.08 ± 2.01 × 104/mg. The purity of primary cultured Schwann cells was 88.4 ± 5.79%. These indicate a successful new method for obtaining Schwann cells of high purity and yield from adult crushed sciatic nerve using bone marrow-derived cells.
authors.affiliation: Department of Orthopedics, Bengbu Medical University Affiliated to First Hospital, Bengbu, Anhui Province, China.
authors.forename: Xiao-Pan
authors.initials: XP
authors.lastname: Wang
authors.affiliation: Department of Orthopedics, Bengbu Medical University Affiliated to First Hospital, Bengbu, Anhui Province, China.
authors.forename: Min
authors.identifier: 0000-0001-5749-3078
authors.initials: M
authors.lastname: Wu
authors.affiliation: Department of Orthopedics, Bengbu Medical University Affiliated to First Hospital, Bengbu, Anhui Province, China.
authors.forename: Jian-Zhong
authors.initials: JZ
authors.lastname: Guan
authors.affiliation: Department of Orthopedics, Bengbu Medical University Affiliated to First Hospital, Bengbu, Anhui Province, China.
authors.forename: Zhao-Dong
authors.initials: ZD
authors.lastname: Wang
authors.affiliation: Department of Orthopedics, Bengbu Medical University Affiliated to First Hospital, Bengbu, Anhui Province, China.
authors.forename: Xu-Bin
authors.initials: XB
authors.lastname: Gao
authors.affiliation: Department of Orthopedics, Bengbu Medical University Affiliated to First Hospital, Bengbu, Anhui Province, China.
authors.forename: Yang-Yang
authors.initials: YY
authors.lastname: Liu
keywords: Schwann cells; bone marrow-derived cells; co-culture; dedifferentiation; glial fibrillary acidic protein; in vitro pre-degeneration; mononuclear cells; nerve regeneration; neural cell adhesion molecule; neural regeneration