title: Characterization of High Affinity Iron Acquisition Systems in Campylobacter jejuni.
abstract: Iron acquisition systems are critical for bacterial pathogenesis and thus have been proposed as attractive targets for iron-dependent pathogen control. Of these systems, high-affinity iron acquisition mediated by siderophore, a small iron chelator, is the most efficient iron-scavenging mechanism in gram-negative bacteria. Campylobacter does not produce any siderophores but has the ability to utilize exogenous siderophores. In particular, the enterobactin (Ent)-mediated iron scavenging is tightly linked to Campylobacter pathogenesis. To date, Ent, a triscatecholate with the highest known affinity for ferric iron, is a well-characterized siderophore used by Campylobacter for iron acquisition during in vivo infection. Here, we describe the key methods used to characterize Ent-mediated high affinity iron acquisition system in Campylobacter jejuni.
authors.affiliation: Department of Animal Science, University of Tennessee, 2506 River Drive, Knoxville, TN, 37996-4574, USA.
authors.forename: Ximin
authors.initials: X
authors.lastname: Zeng
authors.affiliation: Department of Animal Science, University of Tennessee, 2506 River Drive, Knoxville, TN, 37996-4574, USA. jlin6@utk.edu.
authors.forename: Jun
authors.initials: J
authors.lastname: Lin
mesh_terms: D019943:Amino Acid Substitution; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D016123:Campylobacter jejuni / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism / Q000472:pathogenicity; D003227:Conjugation, Genetic; D003470:Culture Media / Q000737:chemistry; D003676:Deferoxamine / Q000494:pharmacology; D004758:Enterobactin / Q000096:biosynthesis; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial; D005816:Genetic Complementation Test; D007501:Iron / Q000378:metabolism*; D016254:Mutagenesis, Insertional; D010957:Plasmids / Q000737:chemistry / Q000378:metabolism*; D011485:Protein Binding; D011956:Receptors, Cell Surface / Q000235:genetics* / Q000378:metabolism; D017262:Siderophores / Q000096:biosynthesis; D014774:Virulence
chemical_list: D001425:Bacterial Outer Membrane Proteins; D003470:Culture Media; D011956:Receptors, Cell Surface; D017262:Siderophores; D004758:Enterobactin; D007501:Iron; D003676:Deferoxamine
keywords: Conjugation; Enterobactin; Growth promotion assay; Insertional mutagenesis; Site-directed amino acid substitution mutagenesis