title: Preparative Purification of Bioactive Compounds fromFlos Chrysanthemi Indiciand Evaluation of Its Antiosteoporosis Effect.
abstract: To understand the material basis and underlying molecular machinery of antiosteoporosis activity of theFlos Chrysanthemi Indici(FCI), the consequences of ethanol extract on the bone loss in mice induced due to ovariectomy (OVX) was evaluated. Also, the antiosteoporosis fraction obtained from the FCI ethanol extract was isolated and purified using a preparative high-speed countercurrent chromatography (HSCCC). Thein vitroimpact of the compounds was investigated on osteoblast proliferation and differentiation. The results revealed that ethyl acetate fraction with robustin vivoantiosteoporosis activity was obtained. The important compounds purified by HSCCC using gradient elution system included acacetin, apigenin, luteolin, and linarin. The four compounds enhanced the differentiation and proliferation of osteoblasts in MC3T3-E1 cells. They also augmented the mRNA levels of runt-related transcription factor 2 (Runx2), osteocalcin (OCN), osteopontin (OPN), and type I collagen (COL I). The AKT signaling pathway was also activated in MC3T3-E1 cells by the four compounds. The present study demonstrated that the antiosteoporosis effects of FCI did not depend on a single component, and HSCCC efficiently isolated and purified the antiosteoporosis bioactive compounds from FCI.
authors.affiliation: Department of Prosthodontics, School & Hospital of Stomatology, Tongji University, Shanghai Engineering Research Center of Tooth Restoration and Regeneration, Shanghai, China.
authors.forename: Jia
authors.identifier: 0000-0003-1262-1851
authors.initials: J
authors.lastname: Li
authors.affiliation: Department of Prosthodontics, School & Hospital of Stomatology, Tongji University, Shanghai Engineering Research Center of Tooth Restoration and Regeneration, Shanghai, China.
authors.forename: Xiaosheng
authors.identifier: 0000-0002-0944-0075
authors.initials: X
authors.lastname: Lin
authors.affiliation: Department of Prosthodontics, School & Hospital of Stomatology, Tongji University, Shanghai Engineering Research Center of Tooth Restoration and Regeneration, Shanghai, China.
authors.forename: Yuping
authors.identifier: 0000-0002-3062-9382
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Prosthodontics, School & Hospital of Stomatology, Tongji University, Shanghai Engineering Research Center of Tooth Restoration and Regeneration, Shanghai, China.
authors.forename: Weicai
authors.identifier: 0000-0002-7709-6771
authors.initials: W
authors.lastname: Liu
authors.affiliation: Department of Prosthodontics, School & Hospital of Stomatology, Tongji University, Shanghai Engineering Research Center of Tooth Restoration and Regeneration, Shanghai, China.
authors.forename: Xiaohui
authors.identifier: 0000-0002-1820-7961
authors.initials: X
authors.lastname: Mi
authors.affiliation: Engineering Research Center of Modern Preparation Technology of TCM, Ministry of Education, Shanghai University of Traditional Chinese Medicine, Shanghai, China.
authors.forename: Jiquan
authors.identifier: 0000-0002-2856-3345
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Department of Prosthodontics, School & Hospital of Stomatology, Tongji University, Shanghai Engineering Research Center of Tooth Restoration and Regeneration, Shanghai, China.
authors.forename: Jiansheng
authors.identifier: 0000-0002-1038-0321
authors.initials: J
authors.lastname: Su