title: Ascorbate Oxidase-Based Amperometric Biosensor for l-Ascorbic Acid Determination in Beverages.
abstract: A novel biosensor for l-ascorbic acid determination in different beverages was elaborated. The ascorbate oxidase enzyme (AAO) fromCucurbitasp., EC 1.10.3.3, was immobilized on a screen-printed carbon electrode with poly(ethylene glycol) (400) diglycidyl ether (PEGDGE) as a crosslinking agent. The standards and samples were measured first with a blank electrode. An inert protein, bovine serum albumin (BSA), was immobilized on the surface of this electrode with PEGDGE. The BSA mass was equivalent to the mass of 10 U of AAO enzyme immobilized on the electrodes (0.021 mg). The linear measuring range for l-ascorbic acid was between 5 and 150 µmol/L. As l-ascorbic acid is a vital vitamin and a common antioxidant used in food industry, fruit juices and vitamin C effervescent tablets were examined. The results were compared to HPLC measurements.
authors.affiliation: Eszterházy Károly College, EGERFOOD Knowledge Centre, Leányka u. 6, HU-3300 Eger, Hungary.
authors.forename: Gábor
authors.initials: G
authors.lastname: Csiffáry
authors.affiliation: Eszterházy Károly College, EGERFOOD Knowledge Centre, Leányka u. 6, HU-3300 Eger, Hungary.
authors.forename: Péter
authors.initials: P
authors.lastname: Fűtő
authors.affiliation: National Agricultural Research and Innovation Centre, Food Science Research Institute, Herman Ottó út 15, HU-1022 Budapest, Hungary.
authors.forename: Nóra
authors.initials: N
authors.lastname: Adányi
authors.affiliation: National Agricultural Research and Innovation Centre, Food Science Research Institute, Herman Ottó út 15, HU-1022 Budapest, Hungary.
authors.forename: Attila
authors.initials: A
authors.lastname: Kiss
keywords: ascorbate oxidase; biosensor; l-ascorbic acid; screen-printed carbon electrodes