title: Inhibition of ERK/MAPK suppresses avian leukosis virus subgroup A and B replication.
abstract: We have previously shown that the extracellular signal-regulated kinase/mitogen-activated protein kinase (ERK/MAPK) pathway contributes to subgroup J avian leukosis virus (ALV-J) replication and tumorigenicity. However, a role for ERK/MAPK signaling in ALV-A and ALV-B replication is unknown. In this study we successfully constructed and recovered a recombinant form of ALV-A strain GD13-1 which showed similarities in growth to the parental wild type virus in vitro. ALV subgroups J, A or B all triggered ERK2 activation in primary CEF cells. ERK/MAPK inhibition markedly suppressed ALV-A and ALV-B replication as shown by extremely low levels of viral transcription and virus protein production. This finding provides evidence that ERK/MAPK signaling responses play important roles in ALV replication and may represent novel drug targets for therapeutic intervention strategies.
authors.affiliation: College of Veterinary Medicine, South China Agricultural University, Guangzhou, 510642, People's Republic of China; National and Regional Joint Engineering Laboratory for Medicament of Zoonosis Prevention and Control, People's Republic of China; Key Laboratory of Veterinary Vaccine Innovation of the Ministry of Agriculture, People's Republic of China; Key Laboratory of Zoonosis Prevention and Control of Guangdong Province, People's Republic of China. Electronic address: daimanman1229@163.com.
authors.forename: Manman
authors.initials: M
authors.lastname: Dai
authors.affiliation: College of Animal Science, South China Agricultural University, Guangzhou, 510642, People's Republic of China. Electronic address: hunanfengmin@163.com.
authors.forename: Min
authors.initials: M
authors.lastname: Feng
authors.affiliation: College of Veterinary Medicine, South China Agricultural University, Guangzhou, 510642, People's Republic of China; National and Regional Joint Engineering Laboratory for Medicament of Zoonosis Prevention and Control, People's Republic of China; Key Laboratory of Veterinary Vaccine Innovation of the Ministry of Agriculture, People's Republic of China; Key Laboratory of Zoonosis Prevention and Control of Guangdong Province, People's Republic of China. Electronic address: mliao@scau.edu.cn.
authors.forename: Ming
authors.initials: M
authors.lastname: Liao
authors.affiliation: College of Veterinary Medicine, South China Agricultural University, Guangzhou, 510642, People's Republic of China; National and Regional Joint Engineering Laboratory for Medicament of Zoonosis Prevention and Control, People's Republic of China; Key Laboratory of Veterinary Vaccine Innovation of the Ministry of Agriculture, People's Republic of China; Key Laboratory of Zoonosis Prevention and Control of Guangdong Province, People's Republic of China. Electronic address: caoweish@scau.edu.cn.
authors.forename: Weisheng
authors.initials: W
authors.lastname: Cao
mesh_terms: D000818:Animals; D001353:Avian Leukosis / Q000378:metabolism* / Q000821:virology*; D001354:Avian Leukosis Virus / Q000187:drug effects* / Q000502:physiology*; D002460:Cell Line; D002478:Cells, Cultured; D002645:Chickens; D005347:Fibroblasts / Q000378:metabolism / Q000821:virology; D005419:Flavonoids; D023061:Gene Order; D005822:Genetic Vectors / Q000235:genetics; D016679:Genome, Viral; D020935:MAP Kinase Signaling System / Q000187:drug effects*; D019950:Mitogen-Activated Protein Kinase 1 / Q000378:metabolism; D047428:Protein Kinase Inhibitors / Q000494:pharmacology*; D014779:Virus Replication / Q000187:drug effects*
chemical_list: D005419:Flavonoids; D047428:Protein Kinase Inhibitors; D019950:Mitogen-Activated Protein Kinase 1; C093973:2-(2-amino-3-methoxyphenyl)-4H-1-benzopyran-4-one
keywords: ALV-A; ALV-B; ERK/MAPK pathway; Virus replication