title: A novel aptameric biosensor based on the self-assembled DNA-WS2nanosheet architecture.
abstract: It has been reported that tungsten disulfide (WS2) can bind single-stranded DNA (ssDNA) with high affinity while it has less affinity toward double stranded DNA (dsDNA). In this work, for the first time, the high affinity between WS2and ssDNA was used to construct stable sensing interface for ATP detection. A DNA sequence with -SH at one end was first immobilized on Au electrode. WS2nanosheets were immobilized on the SH-DNA/Au electrode surface due to the strong affinity between WS2and ssDNA. Then the WS2nanosheets were used to immobilize ATP binding aptamer (ABA) through the high affinity between WS2and ssDNA, too. When ATP reacts with the ABA aptamer, duplex will be formed and dissociated from the WS2nanosheets. On the basis of this, an electrochemical aptasensor for ATP was fabricated. This ATP sensor showed high sensitivity, selectivity and stability due to the unique WS2-ssDNA interactions and the specific aptamer-target recognition. Furthermore, this strategy was generalized to detect Hg2+using a mercury-specific aptamer (MSO). This strategy can be expected to offer a promising approach for designing high-performance electrochemical aptasensors for a spectrum of targets.
authors.affiliation: Beijing Research Center for Information Technology in Agriculture, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China.
authors.forename: Aixue
authors.initials: A
authors.lastname: Li
authors.affiliation: Université de Lyon, ECL, INSA-Lyon, UCBL, CPE, CNRS, INL, UMR 5270, 36 Avenue Guy de Collongue, 69134 Ecully Cedex, France.
authors.forename: Jian
authors.initials: J
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Crystal Materials, Shandong University, Jinan 250100, China.
authors.forename: Jichuan
authors.initials: J
authors.lastname: Qiu
authors.affiliation: State Key Laboratory of Crystal Materials, Shandong University, Jinan 250100, China.
authors.forename: Zhenhuan
authors.initials: Z
authors.lastname: Zhao
authors.affiliation: Beijing Research Center for Information Technology in Agriculture, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China.
authors.forename: Cheng
authors.initials: C
authors.lastname: Wang
authors.affiliation: Beijing Research Center for Information Technology in Agriculture, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China. Electronic address: zhaocj@nercita.org.cn.
authors.forename: Chunjiang
authors.initials: C
authors.lastname: Zhao
authors.affiliation: State Key Laboratory of Crystal Materials, Shandong University, Jinan 250100, China. Electronic address: hongliu@sdu.edu.cn.
authors.forename: Hong
authors.initials: H
authors.lastname: Liu
mesh_terms: D000255:Adenosine Triphosphate / Q000032:analysis / Q000737:chemistry; D052157:Aptamers, Nucleotide / Q000378:metabolism*; D001483:Base Sequence; D015374:Biosensing Techniques / Q000379:methods*; D004247:DNA / Q000737:chemistry* / Q000235:genetics; D004563:Electrochemistry; D004566:Electrodes; D008628:Mercury / Q000032:analysis / Q000737:chemistry; D049329:Nanostructures / Q000737:chemistry*; D017973:Tungsten Compounds / Q000737:chemistry*
chemical_list: D052157:Aptamers, Nucleotide; D017973:Tungsten Compounds; D000255:Adenosine Triphosphate; D004247:DNA; D008628:Mercury
keywords: ATP; Aptamer; Biosensor; Hg(+); Tungsten disulfide