title: Detection of Theileria parva in tissues of cattle undergoing severe East Coast fever disease show significant parasite DNA accumulation in the spleen.
abstract: Infiltration and proliferation of Theileria parva infected lymphocytes in bovine host lymphoid organs is one of the hallmarks of T. parva infection. The relative abundance of parasites within infected host tissues, both lymphoid and non-lymphoid is however unknown. Using quantitative PCR, we have shown that significantly higher concentrations of T. parva DNA are detected in the spleens of cattle undergoing severe disease compared to other organs.
authors.affiliation: Washington State University, Department of Veterinary Microbiology and Pathology, P.O. Box 647040, Pullman, WA 99164-6630, United States. Electronic address: olds@vetmed.wsu.edu.
authors.forename: Cassandra L
authors.initials: CL
authors.lastname: Olds
authors.affiliation: Animal Disease Research Unit, USDA Agricultural Research Service, Pullman, WA 99164-6630, United States. Electronic address: tash_paul@hotmail.com.
authors.forename: Tasha
authors.initials: T
authors.lastname: Paul
authors.affiliation: Animal Disease Research Unit, USDA Agricultural Research Service, Pullman, WA 99164-6630, United States. Electronic address: scoles@vetmed.wsu.edu.
authors.forename: Glen A
authors.initials: GA
authors.lastname: Scoles
mesh_terms: D000818:Animals; D002417:Cattle; D016054:DNA, Protozoan / Q000032:analysis* / Q000378:metabolism; D013154:Spleen / Q000737:chemistry* / Q000469:parasitology; D016797:Theileria parva / Q000502:physiology; D013801:Theileriasis / Q000503:physiopathology*
chemical_list: D016054:DNA, Protozoan
keywords: Nested PCR; Quantitative PCR; Spleen; Theileria parva