title: Using Galleria mellonella as an Infection Model for Campylobacter jejuni Pathogenesis.
abstract: Nonmammalian model systems of infection have been employed recently to study bacterial virulence. For instance, Galleria mellonella (the greater wax moth) has been shown to be susceptible to infection by many bacterial pathogens including the enteric pathogen Campylobacter jejuni. In contrast to the traditional animal models for C. jejuni such as the chick colonization model and ferret diarrheal model, the Galleria mellonella infection model has the advantages of lower cost, ease of use and no animal breeding is required. However, injecting the larvae with bacteria requires care to avoid killing of larvae, which could lead to misleading results. Here, we describe the infection of G. mellonella larvae by C. jejuni and how to record/interpret results.
authors.affiliation: Department of Biochemistry, Microbiology and Immunology, Ottawa Institute of Systems Biology, University of Ottawa, 451 Smyth Road, Ottawa, ON, Canada, K1H 8M5.
authors.forename: Momen
authors.initials: M
authors.lastname: Askoura
authors.affiliation: Department of Biochemistry, Microbiology and Immunology, Ottawa Institute of Systems Biology, University of Ottawa, 451 Smyth Road, Ottawa, ON, Canada, K1H 8M5. astintzi@uottawa.ca.
authors.forename: Alain
authors.initials: A
authors.lastname: Stintzi
mesh_terms: D000818:Animals; D002169:Campylobacter Infections / Q000382:microbiology* / Q000401:mortality / Q000473:pathology; D016123:Campylobacter jejuni / Q000472:pathogenicity* / Q000502:physiology; D004195:Disease Models, Animal; D006458:Hemolymph / Q000382:microbiology; D007814:Larva / Q000382:microbiology*; D007928:Lethal Dose 50; D009036:Moths / Q000382:microbiology*; D016019:Survival Analysis; D014774:Virulence
keywords: Campylobacter jejuni; Galleria mellonella; Hemolymph; Infection; Melanization; Pathogenesis; Survival