title: Controlled Retention and Release of Biomolecular Transport Systems Using Shape-Changing Polymer Bilayers.
abstract: Biomolecular transport systems based on cytoskeletal filaments and motor proteins have become promising tools for a wide range of nanotechnological applications. In this paper, we report control of such transport systems using substrates with switchable shape. We demonstrate this approach on the example of microtubules gliding on surfaces of self-folding polymer bilayers with adsorbed kinesin motors. The polymer bilayers are able to undergo reversible transitions between flat and tube-like shapes that allow the externally controlled retention and release of gliding microtubules. The demonstrated approach, based on surfaces with reconfigurable topography, opens broad perspectives to control biomolecular transport systems for bioanalytical and sensing applications, as well as for the construction of subcellular compartments in the field of synthetic biology.
authors.affiliation: College of Engineering, College of Family and Consumer Sciences, University of Georgia, Athens, GA, 30602, USA.|Leibniz Institute of Polymer Research e.V. Dresden, Hohe Str. 6, 01069, Dresden, Germany.
authors.forename: Georgi
authors.initials: G
authors.lastname: Stoychev
authors.affiliation: B CUBE-Center for Molecular Bioengineering, Technische Universität Dresden and Max-Planck-Institute of Molecular Cell Biology and Genetics, 01307, Dresden, Germany.
authors.forename: Cordula
authors.initials: C
authors.lastname: Reuther
authors.affiliation: B CUBE-Center for Molecular Bioengineering, Technische Universität Dresden and Max-Planck-Institute of Molecular Cell Biology and Genetics, 01307, Dresden, Germany.
authors.forename: Stefan
authors.initials: S
authors.lastname: Diez
authors.affiliation: College of Engineering, College of Family and Consumer Sciences, University of Georgia, Athens, GA, 30602, USA.|Leibniz Institute of Polymer Research e.V. Dresden, Hohe Str. 6, 01069, Dresden, Germany.
authors.forename: Leonid
authors.initials: L
authors.lastname: Ionov
mesh_terms: D001692:Biological Transport; D008051:Lipid Bilayers / Q000737:chemistry / Q000378:metabolism*; D020409:Molecular Motor Proteins / Q000737:chemistry / Q000378:metabolism*; D010316:Particle Size; D011108:Polymers / Q000737:chemistry / Q000378:metabolism*
chemical_list: D008051:Lipid Bilayers; D020409:Molecular Motor Proteins; D011108:Polymers
keywords: biomolecular transport; hydrogels; motor proteins; self-folding