title: Complete Genome Sequence ofClostridium estertheticumDSM 8809, a Microbe Identified in Spoiled Vacuum Packed Beef.
abstract: Blown pack spoilage (BPS) is a major issue for the beef industry. Etiological agents of BPS involve members of a group ofClostridiumspecies, includingClostridium estertheticumwhich has the ability to produce gas, mostly carbon dioxide, under anaerobic psychotrophic growth conditions. This spore-forming bacterium grows slowly under laboratory conditions, and it can take up to 3 months to produce a workable culture. These characteristics have limited the study of this commercially challenging bacterium. Consequently information on this bacterium is limited and no effective controls are currently available to confidently detect and manage this production risk. In this study the complete genome ofC. estertheticumDSM 8809 was determined by SMRT®sequencing. The genome consists of a circular chromosome of 4.7 Mbp along with a single plasmid carrying a potential tellurite resistance genetehBand a Tn3-like resolvase-encoding genetnpR. The genome sequence was searched for central metabolic pathways that would support its biochemical profile and several enzymes contributing to this phenotype were identified. Several putative antibiotic/biocide/metal resistance-encoding genes and virulence factors were also identified in the genome, a feature that requires further research. The availability of the genome sequence will provide a basic blueprint from which to develop valuable biomarkers that could support and improve the detection and control of this bacterium along the beef production chain.
authors.affiliation: UCD-Centre for Food Safety, School of Public Health, Physiotherapy and Sports Science, School of Biomedical and Biomolecular Science, University College Dublin Dublin, Ireland.
authors.forename: Zhongyi
authors.initials: Z
authors.lastname: Yu
authors.affiliation: UCD-Centre for Food Safety, School of Public Health, Physiotherapy and Sports Science, School of Biomedical and Biomolecular Science, University College Dublin Dublin, Ireland.
authors.forename: Lynda
authors.initials: L
authors.lastname: Gunn
authors.affiliation: UCD-Centre for Food Safety, School of Public Health, Physiotherapy and Sports Science, School of Biomedical and Biomolecular Science, University College Dublin Dublin, Ireland.
authors.forename: Evan
authors.initials: E
authors.lastname: Brennan
authors.affiliation: Teagasc Food Research Centre Dublin, Ireland.
authors.forename: Rachael
authors.initials: R
authors.lastname: Reid
authors.affiliation: UCD-Centre for Food Safety, School of Public Health, Physiotherapy and Sports Science, School of Biomedical and Biomolecular Science, University College Dublin Dublin, Ireland.
authors.forename: Patrick G
authors.initials: PG
authors.lastname: Wall
authors.affiliation: UCD-Centre for Food Safety, School of Public Health, Physiotherapy and Sports Science, School of Biomedical and Biomolecular Science, University College Dublin Dublin, Ireland.
authors.forename: Peadar Ó
authors.initials: PÓ
authors.lastname: Gaora
authors.affiliation: UCD-Centre for Food Safety, School of Public Health, Physiotherapy and Sports Science, School of Biomedical and Biomolecular Science, University College Dublin Dublin, Ireland.
authors.forename: Daniel
authors.initials: D
authors.lastname: Hurley
authors.affiliation: Teagasc Food Research Centre Dublin, Ireland.
authors.forename: Declan
authors.initials: D
authors.lastname: Bolton
authors.affiliation: UCD-Centre for Food Safety, School of Public Health, Physiotherapy and Sports Science, School of Biomedical and Biomolecular Science, University College Dublin Dublin, Ireland.
authors.forename: Séamus
authors.initials: S
authors.lastname: Fanning
keywords: Clostridium estertheticum; blown pack spoilage; food quality; vacuum packed beef; whole genome sequencing