title: Map-based cloning and characterization of the novel yellow-green leaf gene ys83 in rice (Oryza sativa).
abstract: Leaf-color mutants have been extensively studied in rice, and many corresponding genes have been identified up to now. However, leaf-color mutation mechanisms are diverse and still need further research through identification of novel genes. In the present paper, we isolated a leaf-color mutant, ys83, in rice (Oryza sativa). The mutant displayed a yellow-green leaf phenotype at seedling stage, and then slowly turned into light-green leaf from late tillering stage. In its yellow leaves, photosynthetic pigment contents significantly decreased and the chloroplast development was retarded. The mutant phenotype was controlled by a recessive mutation in a nuclear gene on the short arm of rice chromosome 2. Map-based cloning and sequencing analysis suggested that the candidate gene was YS83 (LOC_Os02g05890) encoding a protein containing 165 amino acid residues. Gene YS83 was expressed in a wide range of tissues, and its encoded protein was targeted to the chloroplast. In the mutant, a T-to-A substitution occurred in coding sequence of gene YS83, which caused a premature translation of its encoded product. By introduction of the wild-type gene, the ys83 mutant recovered to normal green-leaf phenotype. Taken together, we successfully identified a novel yellow-green leaf gene YS83. In addition, number of productive panicles per plant and number of spikelets per panicle only reduced by 6.7% and 7.6%, respectively, meanwhile its seed setting rate and 1000-grain weight (seed size) were not significantly affected in the mutant, so leaf-color mutant gene ys83 could be used as a trait marker gene in commercial hybrid rice production.
authors.affiliation: Rice Research Institute, Sichuan Agricultural University, Chengdu 611130, China.
authors.forename: Xiaozhi
authors.initials: X
authors.lastname: Ma
authors.affiliation: Rice Research Institute, Sichuan Agricultural University, Chengdu 611130, China; School of Automobile & Transportation, Xihua University, Chengdu 610039, China.
authors.forename: Xiaoqiu
authors.initials: X
authors.lastname: Sun
authors.affiliation: Rice Research Institute, Sichuan Agricultural University, Chengdu 611130, China.
authors.forename: Chunmei
authors.initials: C
authors.lastname: Li
authors.affiliation: Rice Research Institute, Sichuan Agricultural University, Chengdu 611130, China.
authors.forename: Rui
authors.initials: R
authors.lastname: Huan
authors.affiliation: Rice Research Institute, Sichuan Agricultural University, Chengdu 611130, China.
authors.forename: Changhui
authors.initials: C
authors.lastname: Sun
authors.affiliation: Rice Research Institute, Sichuan Agricultural University, Chengdu 611130, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Rice Research Institute, Sichuan Agricultural University, Chengdu 611130, China.
authors.forename: Fuliang
authors.initials: F
authors.lastname: Xiao
authors.affiliation: Rice Research Institute, Sichuan Agricultural University, Chengdu 611130, China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Wang
authors.affiliation: Rice Research Institute, Sichuan Agricultural University, Chengdu 611130, China.
authors.forename: Purui
authors.initials: P
authors.lastname: Chen
authors.affiliation: Rice Research Institute, Sichuan Agricultural University, Chengdu 611130, China.
authors.forename: Furong
authors.initials: F
authors.lastname: Ma
authors.affiliation: Rice Research Institute, Sichuan Agricultural University, Chengdu 611130, China.
authors.forename: Kuan
authors.initials: K
authors.lastname: Zhang
authors.affiliation: Rice Research Institute, Sichuan Agricultural University, Chengdu 611130, China. Electronic address: prwang@sicau.edu.cn.
authors.forename: Pingrong
authors.initials: P
authors.lastname: Wang
authors.affiliation: Rice Research Institute, Sichuan Agricultural University, Chengdu 611130, China. Electronic address: xjdeng2006@aliyun.com.
authors.forename: Xiaojian
authors.initials: X
authors.lastname: Deng
mesh_terms: D002874:Chromosome Mapping; D003001:Cloning, Molecular; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D005816:Genetic Complementation Test; D056426:Genetic Loci; D005819:Genetic Markers; D009154:Mutation / Q000235:genetics; D012275:Oryza / Q000235:genetics*; D010641:Phenotype; D010788:Photosynthesis; D018515:Plant Leaves / Q000235:genetics*; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D019655:Quantitative Trait, Heritable; D060888:Real-Time Polymerase Chain Reaction; D036226:Seedlings / Q000648:ultrastructure; D013347:Subcellular Fractions / Q000378:metabolism
chemical_list: D005819:Genetic Markers; D010940:Plant Proteins
keywords: Chlorophyll deficient; Gene cloning; Rice; Yellow-green leaf mutant