title: A microfluidic paper-based electrochemical biosensor array for multiplexed detection of metabolic biomarkers.
abstract: Paper-based microfluidic devices have emerged as simple yet powerful platforms for performing low-cost analytical tests. This paper reports a microfluidic paper-based electrochemical biosensor array for multiplexed detection of physiologically relevant metabolic biomarkers. Different from existing paper-based electrochemical devices, our device includes an array of eight electrochemical sensors and utilizes a handheld custom-made electrochemical reader (potentiostat) for signal readout. The biosensor array can detect several analytes in a sample solution and produce multiple measurements for each analyte from a single run. Using the device, we demonstrate simultaneous detection of glucose, lactate and uric acid in urine, with analytical performance comparable to that of the existing commercial and paper-based platforms. The paper-based biosensor array and its electrochemical reader will enable the acquisition of high-density, statistically meaningful diagnostic information at the point of care in a rapid and cost-efficient way.
authors.affiliation: Department of Mechanical Engineering, McGill University, 817 Sherbrooke Street West, MD270, Montreal, Quebec, H3A 0C3, Canada.
authors.forename: Chen
authors.initials: C
authors.lastname: Zhao
authors.affiliation: Department of Chemistry, University of Massachusetts-Boston, 100 Morrissey Blvd, Boston, MA 02125, USA.
authors.forename: Martin M
authors.initials: MM
authors.lastname: Thuo
authors.affiliation: Department of Mechanical Engineering, McGill University, 817 Sherbrooke Street West, MD270, Montreal, Quebec, H3A 0C3, Canada.
authors.forename: Xinyu
authors.initials: X
authors.lastname: Liu
keywords: biosensors; disease diagnostics; electrochemical detection; metabolic markers; multiplexing; paper-based microfluidics