title: Label-free fast 3D coherent imaging reveals pancreatic islet micro-vascularization and dynamic blood flow.
abstract: In diabetes, pancreaticβ-cells play a key role. These cells are clustered within structures called islets of Langerhans inside the pancreas and produce insulin, which is directly secreted into the blood stream. The dense vascularization of islets of Langerhans is critical for maintaining a proper regulation of blood glucose homeostasis and is known to be affected from the early stage of diabetes. The deep localization of these islets inside the pancreas in the abdominal cavity renders theirin vivovisualization a challenging task. A fast label-free imaging method with high spatial resolution is required to study the vascular network of islets of Langerhans. Based on these requirements, we developed a label-free and three-dimensional imaging method for observing islets of Langerhans using extended-focus Fourier domain Optical Coherence Microscopy (xfOCM). In addition to structural imaging, this system provides three-dimensional vascular network imaging and dynamic blood flow information within islets of Langerhans. We propose our method to deepen the understanding of the interconnection between diabetes and the evolution of the islet vascular network.
authors.affiliation: Laboratoire d'Optique Biomédicale, Ecole Polytechnique Fédérale de Lausanne (EPFL),1015 Lausanne, Switzerland.
authors.forename: Corinne
authors.initials: C
authors.lastname: Berclaz
authors.affiliation: Laboratoire d'Optique Biomédicale, Ecole Polytechnique Fédérale de Lausanne (EPFL),1015 Lausanne, Switzerland.
authors.forename: Daniel
authors.initials: D
authors.lastname: Szlag
authors.affiliation: Laboratoire d'Optique Biomédicale, Ecole Polytechnique Fédérale de Lausanne (EPFL),1015 Lausanne, Switzerland.
authors.forename: David
authors.initials: D
authors.lastname: Nguyen
authors.affiliation: Laboratoire d'Optique Biomédicale, Ecole Polytechnique Fédérale de Lausanne (EPFL),1015 Lausanne, Switzerland; Hepia, University of Applied Science of Western Switzerland, 1202 Genève, Switzerland.
authors.forename: Jérôme
authors.initials: J
authors.lastname: Extermann
authors.affiliation: Laboratoire d'Optique Biomédicale, Ecole Polytechnique Fédérale de Lausanne (EPFL),1015 Lausanne, Switzerland.
authors.forename: Arno
authors.initials: A
authors.lastname: Bouwens
authors.affiliation: Laboratoire d'Optique Biomédicale, Ecole Polytechnique Fédérale de Lausanne (EPFL),1015 Lausanne, Switzerland.
authors.forename: Paul J
authors.initials: PJ
authors.lastname: Marchand
authors.affiliation: EMV Immunology, Lund University, 22100 Lund, Sweden.
authors.forename: Julia
authors.initials: J
authors.lastname: Nilsson
authors.affiliation: EMV Immunology, Lund University, 22100 Lund, Sweden.
authors.forename: Anja
authors.initials: A
authors.lastname: Schmidt-Christensen
authors.affiliation: EMV Immunology, Lund University, 22100 Lund, Sweden.
authors.forename: Dan
authors.initials: D
authors.lastname: Holmberg
authors.affiliation: Danish Stem Cell Center, 2200 Copenhagen, Denmark.
authors.forename: Anne
authors.initials: A
authors.lastname: Grapin-Botton
authors.affiliation: Laboratoire d'Optique Biomédicale, Ecole Polytechnique Fédérale de Lausanne (EPFL),1015 Lausanne, Switzerland.
authors.forename: Theo
authors.initials: T
authors.lastname: Lasser
keywords: (110.4500) Optical coherence tomography; (110.6880) Three-dimensional image acquisition; (170.1420) Biology; (280.2490) Flow diagnostics