title: Comprehensive characterization of genomic instability in pluripotent stem cells and their derived neuroprogenitor cell lines.
abstract: The genomic integrity of two human pluripotent stem cells and their derived neuroprogenitor cell lines was studied, applying a combination of high-resolution genetic methodologies. The usefulness of combining array-comparative genomic hybridization (aCGH) and multiplex fluorescence in situ hybridization (M-FISH) techniques should be delineated to exclude/detect a maximum of possible genomic structural aberrations. Interestingly, in parts different genomic imbalances at chromosomal and subchromosomal levels were detected in pluripotent stem cells and their derivatives. Some of the copy number variations were inherited from the original cell line, whereas other modifications were presumably acquired during the differentiation and manipulation procedures. These results underline the necessity to study both pluripotent stem cells and their differentiated progeny by as many approaches as possible in order to assess their genomic stability before using them in clinical therapies.
authors.affiliation: Visiting Scientist CnPQ Fellowship, Brazil; Jena University Hospital, Friedrich Schiller University, Institute of Human Genetics, Kollegiengasse 10, D-07743 Jena, Germany.
authors.forename: Nestor Luis
authors.initials: NL
authors.lastname: Lopez Corrales
authors.affiliation: Jena University Hospital, Friedrich Schiller University, Institute of Human Genetics, Kollegiengasse 10, D-07743 Jena, Germany.
authors.forename: Kristin
authors.initials: K
authors.lastname: Mrasek
authors.affiliation: Jena University Hospital, Friedrich Schiller University, Institute of Human Genetics, Kollegiengasse 10, D-07743 Jena, Germany.
authors.forename: Martin
authors.initials: M
authors.lastname: Voigt
authors.affiliation: Jena University Hospital, Friedrich Schiller University, Institute of Human Genetics, Kollegiengasse 10, D-07743 Jena, Germany.
authors.forename: Thomas
authors.initials: T
authors.lastname: Liehr
authors.affiliation: Jena University Hospital, Friedrich Schiller University, Institute of Human Genetics, Kollegiengasse 10, D-07743 Jena, Germany.
authors.forename: Nadezda
authors.initials: N
authors.lastname: Kosyakova
keywords: Array-based comparative genomic hybridization (aCGH); Fluorescence in situ hybridization (FISH); Neuroprogenitor cell lines; Pluripotent stem cells