title: The Effect of Oral Consumption of Probiotics in Prevention of Heart Injury in a Rat Myocardial Infarction Model: a Histopathological, Hemodynamic and Biochemical Evaluation.
abstract: Despite the emerging evidence on beneficial effects of probiotics on the cardiovascular system, their impact on the management of ischemic heart diseases and its possible mechanism have not been elucidated.
  
  
  
  Four viable probiotics bacterial strains, including Bifidobacterium breve, Lactobacillus casei, Lactobacillus bulgaricus and Lactobacillus acidophilus, at the concentrations of 2×106 colony-forming units/ml were orally administered to the rats daily for 14 days before the induction of infarct-like myocardial injury using isoproterenol. Subsequently, 24 h after myocardial injury, the right carotid artery and the left ventricle were catheterized for recording blood pressure and cardiac parameters. At the end of the experiment, the heart was removed for the evaluation of histopathological and biochemical parameters, as well as tumor necrosis factor-alpha (TNF-α) assay.
  
  
  
  The induction of acute myocardial injury resulted in significant (P≤0.01) left ventricular (LV) dysfunction, as shown by an increase in LV end-diastolic pressure and a decrease in LV dp/dt max, LV dp/dt min, LV systolic pressure, and blood pressure, as compared with normal rats. Pretreatment with viable probiotics significantly reduced lipid peroxidation and TNF-α level and improved cardiac function (P<0.01).
  
  
  
  This study shows that viable probiotics have a cardioprotective effect on infarct-like myocardial injury through suppressing TNF-α and oxidative stress damage in a rat model. Probiotic supplements may be used as a new option for prophylaxis in patients at the risk of ischemic heart disease in future.
authors.affiliation: Students' Research Center, School of Medicine, Semnan University of Medical Sciences, Semnan, Iran.|Research Center and Department of Physiology, Faculty of Medicine, Semnan University of Medical Sciences, Semnan, Iran.
authors.forename: Jafar
authors.initials: J
authors.lastname: Sadeghzadeh
authors.affiliation: Research Center and Department of Physiology, Faculty of Medicine, Semnan University of Medical Sciences, Semnan, Iran.
authors.forename: Abedin
authors.initials: A
authors.lastname: Vakili
authors.affiliation: Research Center of Nervous System Stem Cells, Department of Anatomy, Faculty of Medicine, Semnan University of Medical Sciences, Semnan, Iran.
authors.forename: Hamid Reza
authors.initials: HR
authors.lastname: Sameni
authors.affiliation: National Nutrition and Food Technology Research Institute, Faculty of Nutrition Sciences and Food Technology, Department of Immunology, Medical School, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
authors.forename: Mahdi
authors.initials: M
authors.lastname: Shadnoush
authors.affiliation: Department of Biochemistry, Faculty of Medicine, Semnan University of Medical Sciences, Semnan, Iran.
authors.forename: Ahmad-Reza
authors.initials: AR
authors.lastname: Bandegi
authors.affiliation: Research Center and Department of Physiology, Faculty of Medicine, Semnan University of Medical Sciences, Semnan, Iran.
authors.forename: Mahdi
authors.initials: M
authors.lastname: Zahedi Khorasani
mesh_terms: D000284:Administration, Oral; D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D001794:Blood Pressure / Q000187:drug effects; D003971:Diastole / Q000187:drug effects; D004195:Disease Models, Animal; D006321:Heart / Q000187:drug effects / Q000503:physiopathology*; D006339:Heart Rate / Q000187:drug effects; D006439:Hemodynamics / Q000187:drug effects*; D018836:Inflammation Mediators / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D009203:Myocardial Infarction / Q000188:drug therapy* / Q000473:pathology / Q000503:physiopathology*; D018384:Oxidative Stress / Q000187:drug effects; D019936:Probiotics / Q000008:administration & dosage* / Q000494:pharmacology / Q000627:therapeutic use*; D017208:Rats, Wistar; D013599:Systole / Q000187:drug effects; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism; D016277:Ventricular Function, Left / Q000187:drug effects
chemical_list: D015415:Biomarkers; D018836:Inflammation Mediators; D014409:Tumor Necrosis Factor-alpha
keywords: Myocardial injury; Tumor necrosis factor-alpha; Probiotics; Isoproterenol; Oxidative stress