title: Antimicrobial activity of whey protein films supplemented with Lactobacillus sakei cell-free supernatant on fresh beef.
abstract: The aim of this work was to evaluate the antimicrobial activity of whey protein isolate (WPI) films supplemented with Lactobacillus sakei NRRL B-1917 cell-free supernatant on beef inoculated with Escherichia coli ATCC 25922 or Listeria monocytogenes Scott A; additionally, sensory evaluation was performed on wrapped beef cubes. Supernatant concentrates were obtained from Lb. sakei cultures in MRS broth after centrifugation, filtering, and freeze-drying. Films were prepared with WPI (3% w/w), alginate (0.625% w/w), rehydrated supernatant (18 mg/ml), and glycerol. Films were used to wrap beef cubes inoculated with ≈103CFU/g E. coli or L. monocytogenes. Sensory evaluation was carried out on grilled beef wrapped or not with the studied antimicrobial films. During refrigerated storage, antimicrobial films reduced 1.4 log10CFU/g of L. monocytogenes after 120 h, while E. coli decreased 2.3 log10CFU/g after 36 h. Grilled beef wrapped with antimicrobial film was well accepted by panelists, besides scores evidenced no significant differences (p > 0.05) between wrapped and unwrapped beef.
authors.affiliation: Departamento de Ingeniería Química, Alimentos y Ambiental, Universidad de las Américas Puebla, Cholula, Puebla 72810, Mexico.
authors.forename: Silvia Del Carmen
authors.initials: SDC
authors.lastname: Beristain-Bauza
authors.affiliation: Departamento de Ingeniería Química, Alimentos y Ambiental, Universidad de las Américas Puebla, Cholula, Puebla 72810, Mexico.
authors.forename: Emma
authors.initials: E
authors.lastname: Mani-López
authors.affiliation: Departamento de Ingeniería Química, Alimentos y Ambiental, Universidad de las Américas Puebla, Cholula, Puebla 72810, Mexico.
authors.forename: Enrique
authors.initials: E
authors.lastname: Palou
authors.affiliation: Departamento de Ingeniería Química, Alimentos y Ambiental, Universidad de las Américas Puebla, Cholula, Puebla 72810, Mexico. Electronic address: aurelio.lopezm@udlap.mx.
authors.forename: Aurelio
authors.initials: A
authors.lastname: López-Malo
mesh_terms: D000890:Anti-Infective Agents / Q000494:pharmacology*; D015169:Colony Count, Microbial; D003296:Cooking; D003470:Culture Media / Q000737:chemistry; D019453:Escherichia coli O157 / Q000187:drug effects; D005516:Food Microbiology; D018857:Food Packaging / Q000379:methods; D061353:Food Storage; D000070256:Latilactobacillus sakei / Q000737:chemistry / Q000254:growth & development; D008089:Listeria monocytogenes / Q000187:drug effects; D000069466:Red Meat / Q000382:microbiology*; D012677:Sensation; D000067816:Whey Proteins / Q000737:chemistry / Q000494:pharmacology*
chemical_list: D000890:Anti-Infective Agents; D003470:Culture Media; D000067816:Whey Proteins
keywords: Antimicrobial activity; Beef; Films; Lactobacillus sakei cell-free supernatant; Whey protein isolate