title: Effects of light-emitting diode irradiation on the osteogenesis of human umbilical cord mesenchymal stem cells in vitro.
abstract: The aim of this study was to examine the effects of light-emitting diode (LED) photobiomodulation therapy on the proliferation and differentiation of human umbilical cord mesenchymal stem cells (hUMSCs) cultured in osteogenic differentiation medium. HUMSCs were irradiated with an LED light at 620 nm and 2 J/cm2and monitored for cell proliferation and osteogenic differentiation activity. The experiment involved four groups of cells: the control group; the osteogenic group (osteo group); the LED group; the osteogenic + LED group (LED + osteo group). HUMSC proliferation was detected by performing a3-(4,5-dimethylthiazol-2yl)-2,5 diphenyltetrazolium bromide(MTT) assay. Osteogenic activity was evaluated by performing alkaline phosphatase (ALP) and Von Kossa staining, and osteopontin (OPN) gene mRNA expression was evaluated byreverse transcription polymerase chain reaction (RT-PCR). The hUMSCs in the LED + osteo group exhibited a significantly higher proliferation rate than the other subgroups. Additionally, there were greater numbers of ALP-positive cells and Von Kossa nodules in the LED + osteo group. OPN mRNA expression in the LED + osteo group was higher than other subgroups. In conclusion, low levels of LED light at a wavelength of 620 nm enhance the proliferation and osteogenic differentiation of hUMSCs during a long culture period.
authors.affiliation: Department of Orthopaedics, Nanshan Hospital, Guangdong MedicalCollege, Shenzhen Guangdong, 518052, China.
authors.forename: Dazhi
authors.initials: D
authors.lastname: Yang
authors.affiliation: Department of Orthopaedics, Nanshan Hospital, Guangdong MedicalCollege, Shenzhen Guangdong, 518052, China.
authors.forename: Weihong
authors.initials: W
authors.lastname: Yi
authors.affiliation: Department of Orthopaedics, Nanshan Hospital, Guangdong MedicalCollege, Shenzhen Guangdong, 518052, China.
authors.forename: Ertian
authors.initials: E
authors.lastname: Wang
authors.affiliation: Department of Orthopaedics, Nanshan Hospital, Guangdong MedicalCollege, Shenzhen Guangdong, 518052, China.
authors.forename: Min
authors.initials: M
authors.lastname: Wang
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D002454:Cell Differentiation / Q000528:radiation effects*; D049109:Cell Proliferation / Q000528:radiation effects; D015870:Gene Expression / Q000528:radiation effects; D006801:Humans; D054023:Lasers, Semiconductor; D008027:Light; D059630:Mesenchymal Stem Cells / Q000378:metabolism / Q000528:radiation effects*; D010012:Osteogenesis / Q000528:radiation effects*; D053495:Osteopontin / Q000235:genetics; D014470:Umbilical Cord / Q000166:cytology
chemical_list: D053495:Osteopontin; D000469:Alkaline Phosphatase