title: Roseovarius aestuariivivens sp. nov., isolated from a tidal flat.
abstract: A Gram-stain-negative, non-motile, aerobic and coccoid, ovoid or rod-shaped bacterium, designated GHTF-24T, was isolated from a tidal flat on the South Sea, South Korea, and subjected to polyphasic taxonomic study. The novel strain grew optimally at 30 °C, at pH 7.0-8.0 and in the presence of 2.0-3.0 % (w/v) NaCl. In the neighbour-joining phylogenetic tree based on 16S rRNA gene sequences, strain GHTF-24T fell within the clade comprising the type strains of species of the genus Roseovarius. Strain GHTF-24T exhibited 16S rRNA gene sequence similarity values of 97.0 % to each of Roseovariushalotolerans HJ50T and Roseovariuspacificus 81-2T and of 91.6-96.5 % to the type strains of the other species of the genus Roseovarius. Strain GHTF-24T contained Q-10 as the predominant ubiquinone and C18 : 1ω7c as the major fatty acid. The major polar lipids of strain GHTF-24T were phosphatidylcholine, phosphatidylglycerol, diphosphatidylglycerol, one unidentified aminolipid and one unidentified lipid. The DNA G+C content of strain GHTF-24T was 62.3 mol%, and its mean DNA-DNA relatedness values with the type strains of R. halotolerans and R.pacificus were 13-16 %. The differential phenotypic properties, together with the phylogenetic and genetic distinctiveness, revealed that strain GHTF-24T is separated from other recognized species of the genus Roseovarius. On the basis of the data presented, strain GHTF-24T is considered to represent a novel species of the genus Roseovarius, for which the name Roseovarius aestuariivivens sp. nov. is proposed. The type strain is GHTF-24T (=KCTC 52454T=NBRC 112420T).
authors.affiliation: Department of Food Science and Biotechnology, Sungkyunkwan University, Jangan-gu, Suwon, Republic of Korea.
authors.forename: Sooyeon
authors.initials: S
authors.lastname: Park
authors.affiliation: Department of Food Science and Biotechnology, Sungkyunkwan University, Jangan-gu, Suwon, Republic of Korea.
authors.forename: Min-Ji
authors.initials: MJ
authors.lastname: Ha
authors.affiliation: Department of Food Science and Biotechnology, Sungkyunkwan University, Jangan-gu, Suwon, Republic of Korea.
authors.forename: Sun Young
authors.initials: SY
authors.lastname: Yoon
authors.affiliation: University of Science and Technology (UST), 113 Gwahak-ro, Yuseong-gu, Daejeon, Republic of Korea.|Department of Food Science and Biotechnology, Sungkyunkwan University, Jangan-gu, Suwon, Republic of Korea.
authors.forename: Yong-Taek
authors.initials: YT
authors.lastname: Jung
authors.affiliation: Department of Food Science and Biotechnology, Sungkyunkwan University, Jangan-gu, Suwon, Republic of Korea.
authors.forename: Jung-Hoon
authors.initials: JH
authors.lastname: Yoon
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D009693:Nucleic Acid Hybridization; D010743:Phospholipids / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D056910:Republic of Korea; D041902:Rhodobacteraceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D012623:Seawater / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D014451:Ubiquinone / Q000031:analogs & derivatives / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D014451:Ubiquinone; C024989:coenzyme Q10