title: Transcription factors regulating uspA genes in Catharanthus roseus.
abstract: RNA-Seq of the Catharanthus roseus SRA database was done in order to detect putative universal stress proteins (USPs) and their possible controlling factors. Previous analysis indicated the existence and characterization of uspA-like genes. In silico analysis of RNA-Seq database in several plant tissues revealed the possible functions and regulations of some uspA-like transcripts whose transcription factors (TFs) that might drive their expression were detected. BLAST indicated the existence of TF superfamilies erf (ethylene-responsive TF), bHLH (basic helix-loop-helix) and WRKY that might regulate several uspA-like genes. This data was proven via semi-quantitative RT-PCR in four plant tissues. Several of these transcription factor superfamilies are known for their action in the plant defense against biotic and abiotic stresses.
authors.affiliation: Department of Biological Sciences, Faculty of Science, King Abdulaziz University (KAU), P.O. Box 80141, 21589 Jeddah, Saudi Arabia; Department of Genetics, Faculty of Agriculture, Ain Shams University, Cairo, Egypt. Electronic address: bahieldin55@gmail.com.
authors.forename: Ahmed
authors.initials: A
authors.lastname: Bahieldin
authors.affiliation: Department of Biological Sciences, Faculty of Science, King Abdulaziz University (KAU), P.O. Box 80141, 21589 Jeddah, Saudi Arabia. Electronic address: ahmed_atefaig2@yahoo.com.
authors.forename: Ahmed
authors.initials: A
authors.lastname: Atef
authors.affiliation: Department of Biological Sciences, Faculty of Science, King Abdulaziz University (KAU), P.O. Box 80141, 21589 Jeddah, Saudi Arabia; Agricultural Genetic Engineering Research Institute (AGERI), Agriculture Research Center (ARC), Giza, Egypt. Electronic address: ahmedshokry1@gmail.com.
authors.forename: Ahmed M
authors.initials: AM
authors.lastname: Shokry
authors.affiliation: Department of Biological Sciences, Faculty of Science, King Abdulaziz University (KAU), P.O. Box 80141, 21589 Jeddah, Saudi Arabia. Electronic address: Skarim@kau.edu.sa.
authors.forename: Saleh
authors.initials: S
authors.lastname: Al-Karim
authors.affiliation: Department of Biological Sciences, Faculty of Science, King Abdulaziz University (KAU), P.O. Box 80141, 21589 Jeddah, Saudi Arabia. Electronic address: sangtas@mit.edu.
authors.forename: Sanaa G
authors.initials: SG
authors.lastname: Al Attas
authors.affiliation: Department of Biological Sciences, Faculty of Science, King Abdulaziz University (KAU), P.O. Box 80141, 21589 Jeddah, Saudi Arabia; Genetics and Cytology Department, Genetic Engineering and Biotechnology Division, National Research Center, Dokki, Egypt. Electronic address: nouromar71@yahoo.com.
authors.forename: Nour O
authors.initials: NO
authors.lastname: Gadallah
authors.affiliation: Department of Biological Sciences, Faculty of Science, King Abdulaziz University (KAU), P.O. Box 80141, 21589 Jeddah, Saudi Arabia; Department of Genetics, Faculty of Agriculture, Ain Shams University, Cairo, Egypt; Princess Al-Jawhara Al-Brahim Centre of Excellence in Research of Hereditary Disorders (PACER-HD), Faculty of Medicine, King Abdulaziz University (KAU), Jeddah, Saudi Arabia. Electronic address: sedris@aucegypt.edu.
authors.forename: Sherif
authors.initials: S
authors.lastname: Edris
authors.affiliation: Department of Biological Sciences, Faculty of Science, King Abdulaziz University (KAU), P.O. Box 80141, 21589 Jeddah, Saudi Arabia; Genetics and Cytology Department, Genetic Engineering and Biotechnology Division, National Research Center, Dokki, Egypt.
authors.forename: Magdy A
authors.initials: MA
authors.lastname: Al-Kordy
authors.affiliation: Department of Biological Sciences, Faculty of Science, King Abdulaziz University (KAU), P.O. Box 80141, 21589 Jeddah, Saudi Arabia; Department of Genetics, Faculty of Agriculture, Ain Shams University, Cairo, Egypt. Electronic address: m_alkordy@yahoo.com.
authors.forename: Sabah M
authors.initials: SM
authors.lastname: Hassan
authors.affiliation: Department of Biological Sciences, Faculty of Science, King Abdulaziz University (KAU), P.O. Box 80141, 21589 Jeddah, Saudi Arabia; Microbial Genetics Department, Genetic Engineering and Biotechnology Division, National Research Centre, Giza, Egypt. Electronic address: sabmahmoud@yahoo.com.
authors.forename: Salah
authors.initials: S
authors.lastname: Abo-Aba
authors.affiliation: Department of Biological Sciences, Faculty of Science, King Abdulaziz University (KAU), P.O. Box 80141, 21589 Jeddah, Saudi Arabia; Department of Genetics, Faculty of Agriculture, Ain Shams University, Cairo, Egypt. Electronic address: fm_domyati@hotmail.com.
authors.forename: Fotouh M
authors.initials: FM
authors.lastname: El-Domyati
mesh_terms: D030008:Catharanthus / Q000235:genetics*; D003198:Computer Simulation; D030541:Databases, Genetic; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant / Q000235:genetics*; D018749:RNA, Plant / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D013312:Stress, Physiological / Q000235:genetics; D014157:Transcription Factors / Q000235:genetics*; D015854:Up-Regulation / Q000235:genetics
chemical_list: D018749:RNA, Plant; D014157:Transcription Factors
keywords: BHLH; Co-expression; Erf; RNA-Seq; SqRT-PCR; Transcription factor; WRKY