title: Rs3842530 Polymorphism in MicroRNA-205 Host Gene in Lung and Breast Cancer Patients.
abstract: BACKGROUND The expression of miR-205 is closely related to the occurrence, development, and prognosis of lung cancer and breast cancer. However, studies show that it plays opposite roles in different tumor types. Because the expression and regulation of miR-205 are primarily confined to epigenetic areas, whether genetic variation of miR-205 is related to the occurrence or to the development of tumors has not been reported. The aim of this study was to screen genetic variation of miR-205 gene and to investigate its association with the risk and development of lung and breast cancer. MATERIAL AND METHODS Genomic DNA was extracted from cultured tumor cell lines and formalin-fixed and paraffin-embedded lung and breast tissue samples. Bisulfite Clone Sequencing (BCS) and qRT-PCR were employed to detect the DNA methylation status and gene expression of the miR-205 gene, respectively. Genetic variation of miR-205 and miR-205HG were genotyped with PCR-sequencing method. Immunohistochemical analysis for ER, PR, and HER2 was performed on breast tissue samples. RESULTS These results indicate that the functional association of rs3842530 in miR-205HG and lung cancer might provide a possible explanation for the tissue-dependent function of miR-205 in different tumors. CONCLUSIONS These results indicate that the functional association of rs3842530 in miR-205HG and lung cancer might provide a possible explanation for the tissue-dependent function of miR-205 in different tumors.
authors.affiliation: The Second Clinical Medical College, School of Medicine, Nanchang University, Nanchang, Jiangxi, China (mainland).
authors.forename: Fan
authors.initials: F
authors.lastname: Zou
authors.affiliation: The Second Clinic Medical College, School of Medicine, Nanchang University, Nanchang, Jiangxi, China (mainland).
authors.forename: Jizhu
authors.initials: J
authors.lastname: Li
authors.affiliation: The Second Clinic Medical College, School of Medicine, Nanchang University, Nanchang, Jiangxi, China (mainland).
authors.forename: Xiaohua
authors.initials: X
authors.lastname: Jie
authors.affiliation: The Second Clinic Medical College, School of Medicine, Nanchang University, Nanchang, Jiangxi, China (mainland).
authors.forename: Xiong
authors.initials: X
authors.lastname: Peng
authors.affiliation: Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Nanchang University, Nanchang, Jiangxi, China (mainland).
authors.forename: Ruiqi
authors.initials: R
authors.lastname: Fan
authors.affiliation: Department of Pathology, The Fourth Affiliated Hospital, Nanchang University, Nanchang, Jiangxi, China (mainland).
authors.forename: Mengmeng
authors.initials: M
authors.lastname: Wang
authors.affiliation: Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Nanchang University, Nanchang, Jiangxi, China (mainland).
authors.forename: Jiangjie
authors.initials: J
authors.lastname: Wang
authors.affiliation: Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Nanchang University, Nanchang, Jiangxi, China (mainland).
authors.forename: Zhuoqi
authors.initials: Z
authors.lastname: Liu
authors.affiliation: Department of Biochemistry and Molecular Biology, Medical Experimental Teaching Center, Nanchang University, Nanchang, Jiangxi, China (mainland).
authors.forename: Hua
authors.initials: H
authors.lastname: Li
authors.affiliation: Department of Pathology, The Fourth Affiliated Hospital, Nanchang University, Nanchang, Jiangxi, China (mainland).
authors.forename: Huan
authors.initials: H
authors.lastname: Deng
authors.affiliation: Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Nanchang University, Nanchang, Jiangxi, China (mainland).
authors.forename: Xiaohong
authors.initials: X
authors.lastname: Yang
authors.affiliation: Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Nanchang University, Nanchang, Jiangxi, China (mainland).
authors.forename: Daya
authors.initials: D
authors.lastname: Luo
mesh_terms: D001943:Breast Neoplasms / Q000235:genetics* / Q000378:metabolism; D045744:Cell Line, Tumor; D019175:DNA Methylation; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D014644:Genetic Variation; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics* / Q000378:metabolism; D061986:MCF-7 Cells; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D020641:Polymorphism, Single Nucleotide; D011379:Prognosis; D011401:Promoter Regions, Genetic; D012307:Risk Factors
chemical_list: D035683:MicroRNAs; C534425:MIR205, human
keywords: Breast Neoplasms, Male; Lung Neoplasms; MicroRNAs; Polymorphism, Single Nucleotide