title: Analysis of Proliferation of Melanoma Cells and Mesenchymal Stem Cells in Co-Culture and Contribution of Experimental Conditions into Interpretation of the Results.
abstract: A series of experiments on co-culturing of Mel IL melanoma cells and mesenhymal stem cells showed that these cells do not influence proliferation of each other, but we observed weaker adhesion of stromal stem cells to plastic in cocultures where with melanoma cells were grown on mesenhymal stem cells feeder. Cell proliferation was also considerably influenced by experimental conditions, which should be taken into account for correct interpretation of obtained results. The principles of experiments on co-culturing of cancer and stromal cells are formulated that take into account the most important factors influencing cell behavior and minimize the probability of artifact results. It was concluded that co-culturing conditions cells significantly affect the experimental results and can be the source of conflicting conclusions on mutual influence of stromal and cancer cells in vitro.
authors.affiliation: Laboratory of Stem and Progenitor Cell Biology, V. A. Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, Russia. oleg.kandarakov@gmail.com.
authors.forename: O F
authors.initials: OF
authors.lastname: Kandarakov
authors.affiliation: Laboratory of Stem and Progenitor Cell Biology, V. A. Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, Russia.
authors.forename: E E
authors.initials: EE
authors.lastname: Kopantseva
authors.affiliation: Laboratory of Stem and Progenitor Cell Biology, V. A. Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, Russia.
authors.forename: A V
authors.initials: AV
authors.lastname: Belyavsky
mesh_terms: D000273:Adipose Tissue / Q000166:cytology / Q000187:drug effects*; D000818:Animals; D002450:Cell Communication / Q000187:drug effects; D002452:Cell Count; D049109:Cell Proliferation / Q000187:drug effects; D018920:Coculture Techniques; D003470:Culture Media / Q000494:pharmacology*; D003627:Data Interpretation, Statistical; D061252:Feeder Cells / Q000166:cytology / Q000187:drug effects*; D006801:Humans; D008545:Melanoma / Q000378:metabolism* / Q000473:pathology; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000187:drug effects*; D061251:Primary Cell Culture; D051381:Rats; D012878:Skin Neoplasms / Q000378:metabolism* / Q000473:pathology; D014407:Tumor Cells, Cultured
chemical_list: D003470:Culture Media
keywords: co-culture; melanoma; mesenchymal stem cells