title: Pectinase production by Aspergillus giganteus in solid-state fermentation: optimization, scale-up, biochemical characterization and its application in olive-oil extraction.
abstract: The application of pectinases in industrial olive-oil processes is restricted by its production cost. Consequently, new fungal strains able to produce higher pectinase titers are required. The aim of this work was to study the capability of Aspergillus giganteus NRRL10 to produce pectinolytic enzymes by SSF and evaluate the application of these in olive-oil extraction. A. giganteus was selected among 12 strains on the basis of high pectinolytic activity and stability. A mixture composed by wheat bran, orange, and lemon peels was selected as the best substrate for enzyme production. Statistical analyses of the experimental design indicated that pH, temperature, and CaCl2are the main factors that affect the production. Subsequently, different aeration flows were tested in a tray reactor; the highest activity was achieved at 20 L min-1per kilogram of dry substrate (kgds). Finally, the pectinolytic enzymes from A. giganteus improved the oil yield and rheological characteristics without affecting oil chemical properties.
authors.affiliation: Instituto de Investigaciones Biotecnológicas-Instituto Tecnológico de Chascomús (IIB-INTECH), Universidad Nacional de San Martín (UNSAM)-Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), San Martín, Buenos Aires, Argentina. gas.ortiz@gmail.com.
authors.forename: Gastón E
authors.identifier: http://orcid.org/0000-0002-4133-8521
authors.initials: GE
authors.lastname: Ortiz
authors.affiliation: Instituto de Investigaciones Biotecnológicas-Instituto Tecnológico de Chascomús (IIB-INTECH), Universidad Nacional de San Martín (UNSAM)-Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), San Martín, Buenos Aires, Argentina.
authors.forename: María C
authors.initials: MC
authors.lastname: Ponce-Mora
authors.affiliation: Instituto de Investigaciones Biotecnológicas-Instituto Tecnológico de Chascomús (IIB-INTECH), Universidad Nacional de San Martín (UNSAM)-Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), San Martín, Buenos Aires, Argentina.
authors.forename: Diego G
authors.initials: DG
authors.lastname: Noseda
authors.affiliation: Centro de Investigación y Desarrollo en Tecnologías de Industrialización de Alimentos, Instituto Nacional de Tecnología Industrial, Av. General Paz 5445, Edificio 40, San Martín, Buenos Aires, Argentina.
authors.forename: Gabriela
authors.initials: G
authors.lastname: Cazabat
authors.affiliation: Centro de Investigación y Desarrollo en Tecnologías de Industrialización de Alimentos, Instituto Nacional de Tecnología Industrial, Av. General Paz 5445, Edificio 40, San Martín, Buenos Aires, Argentina.
authors.forename: Celina
authors.initials: C
authors.lastname: Saravalli
authors.affiliation: Centro de Investigación y Desarrollo en Tecnologías de Industrialización de Alimentos, Instituto Nacional de Tecnología Industrial, Av. General Paz 5445, Edificio 40, San Martín, Buenos Aires, Argentina.
authors.forename: María C
authors.initials: MC
authors.lastname: López
authors.affiliation: Centro de Investigación y Desarrollo en Tecnologías de Industrialización de Alimentos, Instituto Nacional de Tecnología Industrial, Av. General Paz 5445, Edificio 40, San Martín, Buenos Aires, Argentina.
authors.forename: Guillermo P
authors.initials: GP
authors.lastname: Gil
authors.affiliation: Centro de Investigación y Desarrollo en Biotecnología Industrial, Instituto Nacional de Tecnología Industrial, Av. General Paz 5445, Edificio 51, San Martín, Buenos Aires, Argentina.
authors.forename: Martín
authors.initials: M
authors.lastname: Blasco
authors.affiliation: Instituto de Investigaciones Biotecnológicas-Instituto Tecnológico de Chascomús (IIB-INTECH), Universidad Nacional de San Martín (UNSAM)-Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), San Martín, Buenos Aires, Argentina.
authors.forename: Edgardo O
authors.initials: EO
authors.lastname: Albertó
mesh_terms: D001230:Aspergillus / Q000145:classification / Q000201:enzymology*; D019149:Bioreactors; D055598:Chemical Phenomena; D003470:Culture Media / Q000737:chemistry; D005285:Fermentation; D005511:Food Handling; D006863:Hydrogen-Ion Concentration; D007218:Industrial Microbiology; D000069463:Olive Oil; D011096:Polygalacturonase / Q000096:biosynthesis*; D013696:Temperature
chemical_list: D003470:Culture Media; D000069463:Olive Oil; D011096:Polygalacturonase
keywords: Agricultural waste; Aspergillus giganteus; Design of experiment; Olive oil; Pectinase production; Pectinolytic enzyme; Solid-state fermentation