title: Volasertib suppresses the growth of human hepatocellular carcinomain vitroandin vivo.
abstract: Hepatocellular carcinoma (HCC) is the sixth most frequent malignant tumor with poor prognosis, and its clinical therapeutic outcome is poor. Volasertib, a potent small molecular inhibitor of polo-like kinase 1 (PLK1), is currently tested for treatment of multiple cancers in the clinical trials. However, the antitumor effect of volasertib on HCC is still unknown. In this study, our data show that volasertib is able to induce cell growth inhibition, cell cycle arrest at G2/M phase and apoptosis with the spindle abnormalities in human HCC cells. Furthermore, volasertib also increases the intracellular reactive oxidative species (ROS) levels, and pretreated with ROS scavenger N-acety-L-cysteine partly reverses volasertib-induced apoptosis. Moreover, volasertib markedly inhibits the subcutaneous xenograft growth of HCC in nude mice. Overall, our study provides new therapeutic potential of volasertib on hepatocellular carcinoma.
authors.affiliation: Department of Cell Biology & Institute of Biomedicine, College of Life Science and Technology, Jinan University, National Engineering Research Center of Genetic Medicine, Guangdong Provincial Key Laboratory of Bioengineering Medicine Guangzhou 510632, Guangdong, China.
authors.forename: Di-Wei
authors.initials: DW
authors.lastname: Zheng
authors.affiliation: Department of Cell Biology & Institute of Biomedicine, College of Life Science and Technology, Jinan University, National Engineering Research Center of Genetic Medicine, Guangdong Provincial Key Laboratory of Bioengineering MedicineGuangzhou 510632, Guangdong, China; Center for Clinic Immunology, Third Hospital at Sun Yat-sen UniversityGuangzhou 510630, China.
authors.forename: You-Qiu
authors.initials: YQ
authors.lastname: Xue
authors.affiliation: Department of Gastrointertinal Surgery & General Surgery, Guangdong General Hospital, Guangdong Academy of Medical Sciences Guangzhou, Guangdong 510080, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Li
authors.affiliation: Department of Urology, The 3rd Affiliated Hospital of Sun Yat-sen University Guangzhou, Guangdong 510630, China.
authors.forename: Jin-Ming
authors.initials: JM
authors.lastname: Di
authors.affiliation: Department of Cell Biology & Institute of Biomedicine, College of Life Science and Technology, Jinan University, National Engineering Research Center of Genetic Medicine, Guangdong Provincial Key Laboratory of Bioengineering Medicine Guangzhou 510632, Guangdong, China.
authors.forename: Jian-Ge
authors.initials: JG
authors.lastname: Qiu
authors.affiliation: Department of Cell Biology & Institute of Biomedicine, College of Life Science and Technology, Jinan University, National Engineering Research Center of Genetic Medicine, Guangdong Provincial Key Laboratory of Bioengineering Medicine Guangzhou 510632, Guangdong, China.
authors.forename: Wen-Ji
authors.initials: WJ
authors.lastname: Zhang
authors.affiliation: Department of Cell Biology & Institute of Biomedicine, College of Life Science and Technology, Jinan University, National Engineering Research Center of Genetic Medicine, Guangdong Provincial Key Laboratory of Bioengineering Medicine Guangzhou 510632, Guangdong, China.
authors.forename: Qi-Wei
authors.initials: QW
authors.lastname: Jiang
authors.affiliation: Department of Cell Biology & Institute of Biomedicine, College of Life Science and Technology, Jinan University, National Engineering Research Center of Genetic Medicine, Guangdong Provincial Key Laboratory of Bioengineering Medicine Guangzhou 510632, Guangdong, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Yang
authors.affiliation: Department of Cell Biology & Institute of Biomedicine, College of Life Science and Technology, Jinan University, National Engineering Research Center of Genetic Medicine, Guangdong Provincial Key Laboratory of Bioengineering Medicine Guangzhou 510632, Guangdong, China.
authors.forename: Yao
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Department of Cell Biology & Institute of Biomedicine, College of Life Science and Technology, Jinan University, National Engineering Research Center of Genetic Medicine, Guangdong Provincial Key Laboratory of Bioengineering Medicine Guangzhou 510632, Guangdong, China.
authors.forename: Meng-Ning
authors.initials: MN
authors.lastname: Wei
authors.affiliation: Department of Cell Biology & Institute of Biomedicine, College of Life Science and Technology, Jinan University, National Engineering Research Center of Genetic Medicine, Guangdong Provincial Key Laboratory of Bioengineering Medicine Guangzhou 510632, Guangdong, China.
authors.forename: Jia-Rong
authors.initials: JR
authors.lastname: Huang
authors.affiliation: Department of Cell Biology & Institute of Biomedicine, College of Life Science and Technology, Jinan University, National Engineering Research Center of Genetic Medicine, Guangdong Provincial Key Laboratory of Bioengineering Medicine Guangzhou 510632, Guangdong, China.
authors.forename: Kun
authors.initials: K
authors.lastname: Wang
authors.affiliation: Department of Cell Biology & Institute of Biomedicine, College of Life Science and Technology, Jinan University, National Engineering Research Center of Genetic Medicine, Guangdong Provincial Key Laboratory of Bioengineering Medicine Guangzhou 510632, Guangdong, China.
authors.forename: Xing
authors.initials: X
authors.lastname: Wei
authors.affiliation: Department of Cell Biology & Institute of Biomedicine, College of Life Science and Technology, Jinan University, National Engineering Research Center of Genetic Medicine, Guangdong Provincial Key Laboratory of Bioengineering Medicine Guangzhou 510632, Guangdong, China.
authors.forename: Zhi
authors.initials: Z
authors.lastname: Shi
keywords: Hepatocellular carcinoma; ROS; apoptosis; volasertib