title: Multiplex miRNA assay using lanthanide-tagged probes and the duplex-specific nuclease amplification strategy.
abstract: A multiplex ICP-MS-based miRNA assay with duplex-specific nuclease amplification using bifunctional oligonucleotide probes was proposed. A multiplex assay of miR-141, let-7d, and miR-21 could be achieved simultaneously.
authors.affiliation: Beijing Key Laboratory for Microanalytical Methods and Instrumentation, Department of Chemistry, Tsinghua University, Beijing, 100084, China. sczhang@mail.tsinghua.edu.cn.
authors.forename: Shixi
authors.initials: S
authors.lastname: Zhang
authors.affiliation: College of Chemistry, Sichuan University, Chengdu, 610064, China.
authors.forename: Rui
authors.identifier: http://orcid.org/0000-0001-9928-5373
authors.initials: R
authors.lastname: Liu
authors.affiliation: Beijing Key Laboratory for Microanalytical Methods and Instrumentation, Department of Chemistry, Tsinghua University, Beijing, 100084, China. sczhang@mail.tsinghua.edu.cn.
authors.forename: Zhi
authors.initials: Z
authors.lastname: Xing
authors.affiliation: Beijing Key Laboratory for Microanalytical Methods and Instrumentation, Department of Chemistry, Tsinghua University, Beijing, 100084, China. sczhang@mail.tsinghua.edu.cn.
authors.forename: Sichun
authors.initials: S
authors.lastname: Zhang
authors.affiliation: Beijing Key Laboratory for Microanalytical Methods and Instrumentation, Department of Chemistry, Tsinghua University, Beijing, 100084, China. sczhang@mail.tsinghua.edu.cn.
authors.forename: Xinrong
authors.initials: X
authors.lastname: Zhang
mesh_terms: D028581:Lanthanoid Series Elements / Q000737:chemistry*; D013058:Mass Spectrometry; D035683:MicroRNAs / Q000032:analysis* / Q000235:genetics*; D021141:Nucleic Acid Amplification Techniques; D015345:Oligonucleotide Probes / Q000737:chemistry*
chemical_list: D028581:Lanthanoid Series Elements; D035683:MicroRNAs; D015345:Oligonucleotide Probes