title: Renal Denervation Attenuates Multi-Organ Fibrosis and Improves Vascular Remodeling in Rats with Transverse Aortic Constriction Induced Cardiomyopathy.
abstract: OBJECTIVE
  To investigate the effects of renal denervation (RDN) on multi-organ fibrosis and vascular remodeling in cardiomyopathy.
  
  
  METHODS
  Thirty-six male Sprague-Dawley rats underwent transverse aortic constriction (TAC). Five weeks later, 28 surviving TAC rats were randomly assigned to three groups: (1) RDN, (2) Sham, (3) Carvedilol. Six male Sham TAC rats served as the Control. Ten weeks after TAC, samples were collected.
  
  
  RESULTS
  TAC rats showed an increased diastolic interventricular septal thickness at week 5. At 10 weeks, Masson staining showed that left ventricular and renal glomerular fibrosis were significantly reduced in RDN compared with Sham group. In comparison to Sham group, hepatic perivascular fibrosis was attenuated in both RDN and Carvedilol group, so were the media thickness and the media/lumen of aorta. The plasma levels of B-type natriuretic peptide (BNP), Cystatin C (Cys-C), Alanine Transaminase, angiotensin II (Ang II), transforming growth factor beta 1 (TGF-β1), and malondialdehyde increased, and total superoxide dismutase (T-SOD) decreased in Sham but not in RDN group, compared with Control group. Both RDN and Carvedilol reduced the Cys-C and TGF-β1 levels, and restored T-SOD concentration, compared with Sham group. While only RDN lowered the plasma levels of BNP and Ang II. No significant effects of RDN on blood pressure (BP) and heart rate (HR) were oberved.
  
  
  CONCLUSIONS
  RDN can attenuate multi-organ fibrosis and improve vascular remodeling independent of BP and HR change in TAC-induced cardiomyopathy. These effects of RDN may be associated with the direct inhibition of renin-angiotensin-aldosterone system and oxidative stress.
authors.affiliation: Department of Cardiology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, P.R. China.
authors.forename: Kai
authors.initials: K
authors.lastname: Wang
authors.forename: Dasheng
authors.initials: D
authors.lastname: Lu
authors.forename: Bin
authors.initials: B
authors.lastname: Zhang
authors.forename: Shengchan
authors.initials: S
authors.lastname: Wang
authors.forename: Qian
authors.initials: Q
authors.lastname: Liu
authors.forename: Qi
authors.initials: Q
authors.lastname: Zhang
authors.forename: Jie
authors.initials: J
authors.lastname: Geng
authors.forename: Qijun
authors.initials: Q
authors.lastname: Shan
mesh_terms: D000410:Alanine Transaminase / Q000378:metabolism; D000804:Angiotensin II / Q000378:metabolism; D000818:Animals; D001011:Aorta / Q000187:drug effects / Q000473:pathology* / Q000503:physiopathology*; D001794:Blood Pressure / Q000187:drug effects; D002227:Carbazoles / Q000494:pharmacology; D006332:Cardiomegaly / Q000150:complications / Q000000981:diagnostic imaging / Q000503:physiopathology; D009202:Cardiomyopathies / Q000097:blood / Q000150:complications / Q000473:pathology* / Q000503:physiopathology*; D000077261:Carvedilol; D003250:Constriction; D055316:Cystatin C / Q000097:blood; D003714:Denervation; D005355:Fibrosis; D006339:Heart Rate / Q000187:drug effects; D007668:Kidney / Q000294:innervation*; D008315:Malondialdehyde / Q000378:metabolism; D020097:Natriuretic Peptide, Brain / Q000097:blood; D009928:Organ Specificity; D018384:Oxidative Stress / Q000187:drug effects; D010446:Peptide Fragments / Q000378:metabolism; D011347:Procollagen / Q000378:metabolism; D011412:Propanolamines / Q000494:pharmacology; D017207:Rats, Sprague-Dawley; D013482:Superoxide Dismutase / Q000378:metabolism; D053773:Transforming Growth Factor beta1 / Q000378:metabolism; D066253:Vascular Remodeling
chemical_list: D002227:Carbazoles; D055316:Cystatin C; D010446:Peptide Fragments; D011347:Procollagen; D011412:Propanolamines; D053773:Transforming Growth Factor beta1; C047202:procollagen Type I N-terminal peptide; C041876:procollagen Type III-N-terminal peptide; D000077261:Carvedilol; D000804:Angiotensin II; D020097:Natriuretic Peptide, Brain; D008315:Malondialdehyde; D013482:Superoxide Dismutase; D000410:Alanine Transaminase