title: Recovery of Campylobacter spp. from Food and Environmental Sources.
abstract: The recovery of Campylobacter species from food and environmental sources is challenging due to the slow growth of these bacteria and the need to suppress competing organisms during the isolation procedures. The addition of multiple selective antimicrobials to growth media can negatively impact recovery of some Campylobacter spp. Here, we describe our current method for the isolation of thermotolerant Campylobacter species, mainly C. jejuni and C. coli, from food and environmental samples. We emphasize the use of membrane filtration during plating for the specific isolation of Campylobacter spp. and a reduced use of antimicrobial supplements throughout the whole isolation process.
authors.affiliation: Canadian Food Inspection Agency, 960 Carling Avenue Bldg # 22, Ottawa, ON, Canada, K1A 0Y9. catherine.carrillo@inspection.gc.ca.
authors.forename: Catherine D
authors.initials: CD
authors.lastname: Carrillo
authors.affiliation: Bureau of Microbial Hazards, Health Canada, 251 Sir Frederick Banting Driveway, Ottawa, ON, Canada, K1A 0K9.
authors.forename: Robyn
authors.initials: R
authors.lastname: Kenwell
authors.affiliation: Health Canada, Québec Region, 1001 Saint-Laurent Street West, Longueuil, QC, Canada, J4K 1C7.
authors.forename: Irene
authors.initials: I
authors.lastname: Iugovaz
authors.affiliation: University of Vermont, Extension, 278 South Main Street, Suite 2, St. Albans, VT, 05478, UK.
authors.forename: Omar A
authors.initials: OA
authors.lastname: Oyarzabal
mesh_terms: D000222:Adaptation, Physiological; D000581:Amidohydrolases / Q000235:genetics; D000818:Animals; D001222:Aspartate Kinase / Q000235:genetics; D001426:Bacterial Proteins / Q000235:genetics*; D017000:Campylobacter coli; D016123:Campylobacter jejuni; D002645:Chickens; D015169:Colony Count, Microbial; D003470:Culture Media / Q000737:chemistry; D017931:DNA Primers / Q000138:chemical synthesis / Q000378:metabolism; D005374:Filtration / Q000379:methods; D005516:Food Microbiology; D015870:Gene Expression; D006358:Hot Temperature; D008460:Meat / Q000382:microbiology*; D008892:Milk / Q000382:microbiology*; D060885:Multiplex Polymerase Chain Reaction; D010461:Peptones / Q000737:chemistry; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D014871:Water Microbiology
chemical_list: D001426:Bacterial Proteins; D003470:Culture Media; D017931:DNA Primers; D010461:Peptones; D012336:RNA, Ribosomal, 16S; D001222:Aspartate Kinase; D000581:Amidohydrolases; C019672:aminoacylase I
keywords: Bolton broth; Buffered peptone water; Campylobacter; Nitrocellulose membrane filter; Poultry; Produce; Raw milk