title: In vitroandin vivoevaluation of electrospun PCL/PMMA fibrous scaffolds for bone regeneration.
abstract: Scaffolds were fabricated by electrospinning using polycaprolactone (PCL) blended with poly(methyl methacrylate) (PMMA) in ratios of 10/0, 7/3, 5/5 and 3/7. The PCL/PMMA ratio affected the fiber diameter, contact angle, tensile strength and biologicalin vitroandin vivoproperties of the scaffolds, and the 7/3 ratio resulted in a higher mechanical strength than 5/5 and 3/7.In vitrocytotoxicity and proliferation of MG-63 osteoblast cells on these blended scaffolds were examined by MTT assay, and it was found that PCL/PMMA blends are suitable for osteoblast cell proliferation. Confocal images and expression of proliferating cell nuclear antigen confirmed the good proliferation and expression of cells on the 7/3 PCL/PMMA fibrous scaffolds.In vivobone formation was examined using rat models, and bone formation was observed on the 7/3 PCL/PMMA scaffold within 2 months.In vitroandin vivoresults suggest that 7/3 PCL/PMMA scaffolds can be used for bone tissue regeneration.
authors.affiliation: Department of Biomedical Engineering and Materials, College of Medicine, Soonchunhyang University, 366-1, Ssangyong-dong, Cheonan, Chungnam 330-090, Republic of Korea.
authors.forename: So-Ra
authors.initials: SR
authors.lastname: Son
authors.affiliation: Department of Biomedical Engineering and Materials, College of Medicine, Soonchunhyang University, 366-1, Ssangyong-dong, Cheonan, Chungnam 330-090, Republic of Korea.
authors.forename: Nguyen-Thuy Ba
authors.initials: NB
authors.lastname: Linh
authors.affiliation: Department of Physiology, College of Medicine, Soonchunhyang University, 366-1, Ssangyong-dong, Cheonan, Chungnam 330-090, Republic of Korea.
authors.forename: Hun-Mo
authors.initials: HM
authors.lastname: Yang
authors.affiliation: Department of Biomedical Engineering and Materials, College of Medicine, Soonchunhyang University, 366-1, Ssangyong-dong, Cheonan, Chungnam 330-090, Republic of Korea.
authors.forename: Byong-Taek
authors.initials: BT
authors.lastname: Lee
keywords: PCL; PMMA; bone regeneration; electrospinning