title: Modular delivery of CpG-incorporated lipid-DNA nanoparticles for spleen DC activation.
abstract: We introduce a versatile carrier system for in vitro and in vivo immune stimulation based on soft matter DNA nanoparticles (NPs). The incorporation of lipid-modified nucleotides into DNA strands enables the formation of micelles of uniform size. In a single self-assembly step, the micelles can be equipped with immune adjuvant (CpG) motifs and fluorescent probes. The immunological effects of CpG confined at the NP surface were studied in a comprehensive manner in animal experiments. Dose-dependent activation of spleen dendritic cells (DCs) by CpG-conjugated NP was observed, which was accompanied by the pronounced up-regulation of co-stimulatory molecule and cytokine production.
authors.affiliation: Shanghai Public Health Clinical Center, Shanghai Medical College, Fudan University, Shanghai 201508, China.
authors.forename: Jun-O
authors.initials: JO
authors.lastname: Jin
authors.affiliation: Department of Chemistry, Pukyong National University, 45 Yongso-ro, Nam-gu, Busan 48513, Republic of Korea.
authors.forename: Haein
authors.initials: H
authors.lastname: Park
authors.affiliation: Shanghai Public Health Clinical Center, Shanghai Medical College, Fudan University, Shanghai 201508, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.affiliation: University of Groningen, Zernike Institute for Advanced Materials, Department of Polymer Chemistry, Nijenborgh 4, 9747 AG Groningen, The Netherlands.
authors.forename: Jan Willem
authors.initials: JW
authors.lastname: de Vries
authors.affiliation: University of Groningen, Zernike Institute for Advanced Materials, Department of Polymer Chemistry, Nijenborgh 4, 9747 AG Groningen, The Netherlands.
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Gruszka
authors.affiliation: Department of Chemistry, Pukyong National University, 45 Yongso-ro, Nam-gu, Busan 48513, Republic of Korea.
authors.forename: Myung Won
authors.initials: MW
authors.lastname: Lee
authors.affiliation: Center for Theragnosis, Biomedical Research Institute, Korea Institute of Science and Technology, 5 Hwarangno 14-gil, Seongbuk-gu, Seoul 02792, Republic of Korea.
authors.forename: Dae-Ro
authors.initials: DR
authors.lastname: Ahn
authors.affiliation: University of Groningen, Zernike Institute for Advanced Materials, Department of Polymer Chemistry, Nijenborgh 4, 9747 AG Groningen, The Netherlands. Electronic address: a.herrmann@rug.nl.
authors.forename: Andreas
authors.initials: A
authors.lastname: Herrmann
authors.affiliation: Department of Chemistry, Pukyong National University, 45 Yongso-ro, Nam-gu, Busan 48513, Republic of Korea. Electronic address: mkwak@pknu.ac.kr.
authors.forename: Minseok
authors.initials: M
authors.lastname: Kwak
mesh_terms: D000276:Adjuvants, Immunologic / Q000008:administration & dosage / Q000737:chemistry; D000818:Animals; D002478:Cells, Cultured; D018899:CpG Islands / Q000235:genetics*; D016207:Cytokines / Q000276:immunology; D004247:DNA / Q000008:administration & dosage* / Q000235:genetics*; D003713:Dendritic Cells / Q000166:cytology / Q000187:drug effects / Q000276:immunology*; D008055:Lipids / Q000737:chemistry*; D008081:Liposomes / Q000008:administration & dosage / Q000737:chemistry; D051379:Mice; D008810:Mice, Inbred C57BL; D053769:Nanocapsules / Q000737:chemistry*; D013154:Spleen / Q000166:cytology / Q000187:drug effects / Q000276:immunology*; D014162:Transfection / Q000379:methods
chemical_list: D000276:Adjuvants, Immunologic; D016207:Cytokines; D008055:Lipids; D008081:Liposomes; D053769:Nanocapsules; D004247:DNA
keywords: Adjuvants; DNA nanotechnology; Dendritic cells; Immunology; Nanomedicine