title: Critical role of superoxide anions and hydroxyl radicals in HEMA-induced apoptosis.
abstract: Resin monomers such as 2-hydroxyethyl methacrylate (HEMA) induce apoptosis because of the excess formation of reactive oxygen species (ROS). The portion of ROS including superoxide anions, hydrogen peroxide or hydroxyl radicals in monomer-induced apoptosis is unknown. Here, the effectiveness of superoxide anions or hydroxyl radicals was analyzed using tempol or sodium formate as radical scavengers.
  
  
  
  RAW264.7 mouse macrophages were exposed to HEMA (0-6-8mM) in the presence of tempol (0-0.05-0.5-5.0mM) or sodium formate (0-1-5-10mM). The formation of ROS using DCFH2-DA or dihydrorhodamine 123 (DHR123) as fluorescent dyes and the induction of apoptosis was determined by flow cytometry after 1h or 24h exposure periods. Expression of enzymes related to ROS metabolism was detected by Western blotting.
  
  
  
  DCF fluorescence significantly increased after short exposure (1h) while DHR123 fluorescence was enhanced after a long exposure period (24h) in cells treated with HEMA. Although no influence was detected on the formation of ROS, tempol or sodium formate protected cells from HEMA-induced apoptosis. The number of cells in late apoptosis or necrosis induced with 6 or 8mM HEMA was reduced in the presence of tempol or low concentrations of sodium formate. HEMA-induced expression of catalase, indicating oxidative stress, decreased in the presence of tempol.
  
  
  
  Superoxide anions and hydroxyl radicals contribute to HEMA-induced apoptosis. The current findings support the development of strategies based on the pharmacological inhibition of enzymes producing superoxide anions finally converted to hydroxyl radicals to compensate for potential adverse tissue reactions associated with dental composites.
authors.affiliation: Department of Operative Dentistry and Periodontology, University Hospital Regensburg, University of Regensburg, D-93042 Regensburg, Germany. Electronic address: helmut.schweikl@klinik.uni-regensburg.de.
authors.forename: H
authors.initials: H
authors.lastname: Schweikl
authors.affiliation: Department of Operative Dentistry and Periodontology, University Hospital Regensburg, University of Regensburg, D-93042 Regensburg, Germany.
authors.forename: M
authors.initials: M
authors.lastname: Godula
authors.affiliation: Department of Operative Dentistry and Periodontology, University Hospital Regensburg, University of Regensburg, D-93042 Regensburg, Germany.
authors.forename: C
authors.initials: C
authors.lastname: Petzel
authors.affiliation: Department of Operative Dentistry and Periodontology, University Hospital Regensburg, University of Regensburg, D-93042 Regensburg, Germany.
authors.forename: C
authors.initials: C
authors.lastname: Bolay
authors.affiliation: Department of Operative Dentistry and Periodontology, University Hospital Regensburg, University of Regensburg, D-93042 Regensburg, Germany.
authors.forename: K A
authors.initials: KA
authors.lastname: Hiller
authors.affiliation: Department of Operative Dentistry and Periodontology, University Hospital Regensburg, University of Regensburg, D-93042 Regensburg, Germany.
authors.forename: W
authors.initials: W
authors.lastname: Buchalla
mesh_terms: D000818:Animals; D017209:Apoptosis; D017665:Hydroxyl Radical; D008264:Macrophages; D008689:Methacrylates; D051379:Mice; D017382:Reactive Oxygen Species; D013481:Superoxides
chemical_list: D008689:Methacrylates; D017382:Reactive Oxygen Species; D013481:Superoxides; D017665:Hydroxyl Radical; C005044:hydroxyethyl methacrylate
keywords: Apoptosis; HEMA; Resin monomer; Sodium formate; Tempol