title: Molecular visualizing and quantifying immune-associated peroxynitrite fluxes in phagocytes and mouse inflammation model.
abstract: Reactions of peroxynitrite (ONOO-) with biomolecules can lead to cytotoxic and cytoprotective events. Due to the difficulty of directly and unambiguously measuring its levels, most of the beneficial effects associated with ONOO-in vivo remain controversial or poorly characterized. Recently, optical imaging has served as a powerful noninvasive approach to studying ONOO-in living systems. However, ratiometric probes for ONOO-are currently lacking. Herein, we report the design, synthesis, and biological evaluation of F482, a novel fluorescence indicator that relies on ONOO--induced diene oxidation. The remarkable sensitivity, selectivity, and photostability of F482enabled us to visualize basal ONOO-in immune-stimulated phagocyte cells and quantify its generation in phagosomes by high-throughput flow cytometry analysis. With the aid of in vivo ONOO-imaging in a mouse inflammation model assisted by F482, we envision that F482will find widespread applications in the study of the ONOO-biology associated with physiological and pathological processes in vitro and in vivo.
authors.affiliation: State key Laboratory of Coordination Chemistry, School of Chemistry and Chemical Engineering, Collaborative Innovation Center of Advanced Microstructures, Nanjing University, Nanjing 210093, PR China.
authors.forename: Zan
authors.initials: Z
authors.lastname: Li
authors.affiliation: State key Laboratory of Coordination Chemistry, School of Chemistry and Chemical Engineering, Collaborative Innovation Center of Advanced Microstructures, Nanjing University, Nanjing 210093, PR China; Department of Pharmacology, Jiangsu Province Hospital of TCM, Nanjing 210029, PR China.
authors.forename: Shi-Hai
authors.initials: SH
authors.lastname: Yan
authors.affiliation: State key Laboratory of Coordination Chemistry, School of Chemistry and Chemical Engineering, Collaborative Innovation Center of Advanced Microstructures, Nanjing University, Nanjing 210093, PR China.
authors.forename: Chen
authors.initials: C
authors.lastname: Chen
authors.affiliation: State key Laboratory of Coordination Chemistry, School of Chemistry and Chemical Engineering, Collaborative Innovation Center of Advanced Microstructures, Nanjing University, Nanjing 210093, PR China. Electronic address: gengzr@nju.edu.cn.
authors.forename: Zhi-Rong
authors.initials: ZR
authors.lastname: Geng
authors.affiliation: State key Laboratory of Coordination Chemistry, School of Chemistry and Chemical Engineering, Collaborative Innovation Center of Advanced Microstructures, Nanjing University, Nanjing 210093, PR China.
authors.forename: Jia-Yin
authors.initials: JY
authors.lastname: Chang
authors.affiliation: State key Laboratory of Coordination Chemistry, School of Chemistry and Chemical Engineering, Collaborative Innovation Center of Advanced Microstructures, Nanjing University, Nanjing 210093, PR China.
authors.forename: Chun-Xia
authors.initials: CX
authors.lastname: Chen
authors.affiliation: State key Laboratory of Coordination Chemistry, School of Chemistry and Chemical Engineering, Collaborative Innovation Center of Advanced Microstructures, Nanjing University, Nanjing 210093, PR China.
authors.forename: Bing-Huan
authors.initials: BH
authors.lastname: Huang
authors.affiliation: State key Laboratory of Coordination Chemistry, School of Chemistry and Chemical Engineering, Collaborative Innovation Center of Advanced Microstructures, Nanjing University, Nanjing 210093, PR China. Electronic address: wangzl@nju.edu.cn.
authors.forename: Zhi-Lin
authors.initials: ZL
authors.lastname: Wang
mesh_terms: D000818:Animals; D015374:Biosensing Techniques; D004195:Disease Models, Animal; D007249:Inflammation / Q000175:diagnosis* / Q000276:immunology; D051379:Mice; D030421:Peroxynitrous Acid / Q000737:chemistry / Q000302:isolation & purification*; D010586:Phagocytes / Q000737:chemistry / Q000276:immunology
chemical_list: D030421:Peroxynitrous Acid
keywords: Inflammation; Knoevenagel condensation; ONOO(−); Phagocytes; Quantifying; Visualizing