title: Isolation, Characterization, and Bioinformatic Analyses of Lytic Salmonella Enteritidis Phages and Tests of Their Antibacterial Activity in Food.
abstract: Salmonella Enteritidis remains a major threat for food safety. To take efforts to develop phage-based biocontrol for S. Enteritidis contamination in food, in this study, the phages against S. Enteritidis were isolated from sewage samples, characterized by host range assays, DNA restriction enzyme pattern analyses, and transmission electron microscope observations, and tested for antibacterial activity in food; some potent phages were further characterized by bioinformatic analyses. Results showed that based on the plaque quality and host range, seven lytic phages targeting S. Enteritidis were selected, considered as seven distinct phages through DNA physical maps, and classified as Myoviridae or Siphoviridae family by morphologic observations; the combined use of such seven strain phages as a "food additive" could succeed in controlling the artificial S. Enteritidis contamination in the different physical forms of food at a range of temperatures; by bioinformatic analyses, both selected phage BPS11Q3and BPS15Q2seemed to be newfound obligate lytic phage strains with no indications for any potentially harmful genes in their genomes. In conclusion, our results showed a potential of isolated phages as food additives for controlling S. Enteritidis contamination in some salmonellosis outbreak-associated food vehicles, and there could be minimized potential risk associated with using BPS11Q3and BPS15Q2in food.
authors.affiliation: Key Laboratory of Guizhou Bioresource Development and Utilization, Guizhou Education University, Guiyang, 550018, Guizhou, China.|Key Lab of Food Quality and Safety of Jiangsu Province-State Key Laboratory Breeding Base, Institute of Food Safety of Jiangsu Academy of Agricultural Science, Nanjing, 210014, Jiangsu, China.
authors.forename: Han
authors.initials: H
authors.lastname: Han
authors.affiliation: Key Laboratory of Guizhou Bioresource Development and Utilization, Guizhou Education University, Guiyang, 550018, Guizhou, China.
authors.forename: Xiaoting
authors.initials: X
authors.lastname: Wei
authors.affiliation: Key Laboratory of Guizhou Bioresource Development and Utilization, Guizhou Education University, Guiyang, 550018, Guizhou, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Wei
authors.affiliation: Key Laboratory of Guizhou Bioresource Development and Utilization, Guizhou Education University, Guiyang, 550018, Guizhou, China.
authors.forename: Xiufeng
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Guizhou Bioresource Development and Utilization, Guizhou Education University, Guiyang, 550018, Guizhou, China.
authors.forename: Xuemin
authors.initials: X
authors.lastname: Li
authors.affiliation: Key Laboratory of Guizhou Bioresource Development and Utilization, Guizhou Education University, Guiyang, 550018, Guizhou, China.
authors.forename: Jinzhong
authors.initials: J
authors.lastname: Jiang
authors.affiliation: Key Lab of Food Quality and Safety of Jiangsu Province-State Key Laboratory Breeding Base, Institute of Food Safety of Jiangsu Academy of Agricultural Science, Nanjing, 210014, Jiangsu, China. wangranhanhan@126.com.
authors.forename: Ran
authors.initials: R
authors.lastname: Wang
mesh_terms: D001433:Bacteriolysis; D019295:Computational Biology; D004279:DNA, Viral / Q000235:genetics; D005516:Food Microbiology; D023061:Gene Order; D005814:Genes, Viral; D016679:Genome, Viral; D058507:Host Specificity; D046529:Microscopy, Electron, Transmission; D017900:Myoviridae / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology / Q000648:ultrastructure; D015183:Restriction Mapping; D012483:Salmonella Phages / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology* / Q000648:ultrastructure; D012477:Salmonella enteritidis / Q000254:growth & development / Q000821:virology*; D012722:Sewage / Q000821:virology; D017906:Siphoviridae / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology / Q000648:ultrastructure; D010948:Viral Plaque Assay; D014771:Virion / Q000648:ultrastructure
chemical_list: D004279:DNA, Viral; D012722:Sewage