title: Hyperinvasiveness of Salmonella enterica serovar Choleraesuis linked to hyperexpression of type III secretion systems in vitro.
abstract: Salmonella enterica serovars Choleraesuis and Typhimurium are among the non-typhoid Salmonella serovars that are important zoonotic pathogens. In clinical observation, S. Typhimurium typically causes diarrheal diseases; however, S. Choleraesuis shows high predilection to cause bacteremia. The mechanism why S. Choleraesuis is more invasive to humans remains unknown. In this study, we compared the S. Typhimurium LT2 and S. Choleraesuis SC-B67 proteomes through stable isotope labeling of amino acid in cell culture (SILAC). In SILAC, the expression of many virulence proteins in two type III secretion systems (T3SSs) were significantly higher in S. Choleraesuis than in S. Typhimurium. Similar differences were also found at the transcriptional level. Compared to S. Typhimurium, S. Choleraesuis showed a higher penetration level to Caco-2 (>100-fold) and MDCK (>10-fold) monolayers. In mice after oral challenge, the invasion of spleen and liver was also higher in S. Choleraesuis than in S. Typhimurium. The transcription of hilD in S. Choleraesuis was increased in physiological (1 mM) or high (10 mM) concentrations of Mg2+, but not in low (8 μM) concentration. We conclude that S. Choleraesuis showed hyperinvasiveness in cellular as well as mouse models due to hyperexpression of T3SS genes.
authors.affiliation: Graduate Institute of Biomedical Sciences, Chang Gung University College of Medicine, Taoyuan, Taiwan.|Molecular Infectious Disease Research Center, Chang Gung Memorial Hospital, Chang Gung University College of Medicine, Taoyuan, Taiwan.
authors.forename: Kuan-Yeh
authors.initials: KY
authors.lastname: Huang
authors.affiliation: Molecular Infectious Disease Research Center, Chang Gung Memorial Hospital, Chang Gung University College of Medicine, Taoyuan, Taiwan.
authors.forename: Yi-Hsin
authors.initials: YH
authors.lastname: Wang
authors.affiliation: Graduate Institute of Biomedical Sciences, Chang Gung University College of Medicine, Taoyuan, Taiwan.
authors.forename: Kun-Yi
authors.initials: KY
authors.lastname: Chien
authors.affiliation: Molecular Infectious Disease Research Center, Chang Gung Memorial Hospital, Chang Gung University College of Medicine, Taoyuan, Taiwan.
authors.forename: Rajendra Prasad
authors.initials: RP
authors.lastname: Janapatla
authors.affiliation: Graduate Institute of Biomedical Sciences, Chang Gung University College of Medicine, Taoyuan, Taiwan.|Molecular Infectious Disease Research Center, Chang Gung Memorial Hospital, Chang Gung University College of Medicine, Taoyuan, Taiwan.
authors.forename: Cheng-Hsun
authors.initials: CH
authors.lastname: Chiu
mesh_terms: D000143:Acids / Q000494:pharmacology; D000818:Animals; D001426:Bacterial Proteins / Q000378:metabolism; D004285:Dogs; D004847:Epithelial Cells / Q000382:microbiology; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects; D005816:Genetic Complementation Test; D006367:HeLa Cells; D006801:Humans; D008264:Macrophages / Q000187:drug effects / Q000382:microbiology; D061985:Madin Darby Canine Kidney Cells; D008274:Magnesium / Q000494:pharmacology; D051379:Mice; D050296:Microbial Viability / Q000187:drug effects; D019779:Salmonella enterica / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism* / Q000472:pathogenicity*; D000069296:Type III Secretion Systems / Q000378:metabolism*; D037521:Virulence Factors / Q000378:metabolism
chemical_list: D000143:Acids; D001426:Bacterial Proteins; D000069296:Type III Secretion Systems; D037521:Virulence Factors; D008274:Magnesium