title: Identification of aSolanum pennelliiChromosome 4 Fruit Flavor and Nutritional Quality-Associated Metabolite QTL.
abstract: A major resource for tomato quality improvement and gene discovery is the collection of introgression lines (ILs) of cultivatedSolanum lycopersicumthat contain different, defined chromosomal segments derived from the wild tomato relative,S. pennellii.Among these lines, IL4-4, in which the bottom ofS. lycopersicum(cv. M82) chromosome 4 is replaced by the correspondingS. pennelliisegment, is altered in many primary and secondary metabolites, including many related to fruit flavor and nutritional quality. Here, we provide a comprehensive profile of IL4-4 ripe fruit metabolites, the transcriptome and fine mapping of sub-ILs. Remarkably, out of 327 quantified metabolites, 185 were significantly changed in IL4-4 fruit, compared to the control. These altered metabolites include volatile organic compounds, primary and secondary metabolites. Partial least squares enhanced discriminant analysis of the metabolite levels among sub-ILs indicated that a genome region encompassing 20 putative open reading frames is responsible for most of the metabolic changes in IL4-4 fruit. This work provides comprehensive insights into IL4-4 fruit biochemistry, identifying a small region of the genome that has major effects on a large and diverse set of metabolites.
authors.affiliation: Horticultural Sciences Department, Genetics Institute, University of Florida, Gainesville FL, USA.
authors.forename: Zhongyuan
authors.initials: Z
authors.lastname: Liu
authors.affiliation: Max Planck Institute of Molecular Plant Physiology Potsdam-Golm, Germany.
authors.forename: Saleh
authors.initials: S
authors.lastname: Alseekh
authors.affiliation: Max Planck Institute of Molecular Plant PhysiologyPotsdam-Golm, Germany; Department of Life Sciences, Ben-Gurion University of the NegevBeersheba, Israel.
authors.forename: Yariv
authors.initials: Y
authors.lastname: Brotman
authors.affiliation: US Department of Agriculture-Agricultural Research Service Robert W. Holley Center for Agriculture and Health, Cornell University, IthacaNY, USA; Robert W. Holley Center for Agriculture and Health, Cornell University and US Department of Agriculture-Agricultural Research Service, IthacaNY, USA.
authors.forename: Yi
authors.initials: Y
authors.lastname: Zheng
authors.affiliation: US Department of Agriculture-Agricultural Research Service Robert W. Holley Center for Agriculture and Health, Cornell University, IthacaNY, USA; Robert W. Holley Center for Agriculture and Health, Cornell University and US Department of Agriculture-Agricultural Research Service, IthacaNY, USA.
authors.forename: Zhangjun
authors.initials: Z
authors.lastname: Fei
authors.affiliation: Horticultural Sciences Department, Genetics Institute, University of Florida, Gainesville FL, USA.
authors.forename: Denise M
authors.initials: DM
authors.lastname: Tieman
authors.affiliation: US Department of Agriculture-Agricultural Research Service Robert W. Holley Center for Agriculture and Health, Cornell University, IthacaNY, USA; Robert W. Holley Center for Agriculture and Health, Cornell University and US Department of Agriculture-Agricultural Research Service, IthacaNY, USA.
authors.forename: James J
authors.initials: JJ
authors.lastname: Giovannoni
authors.affiliation: Max Planck Institute of Molecular Plant Physiology Potsdam-Golm, Germany.
authors.forename: Alisdair R
authors.initials: AR
authors.lastname: Fernie
authors.affiliation: Horticultural Sciences Department, Genetics Institute, University of Florida, Gainesville FL, USA.
authors.forename: Harry J
authors.initials: HJ
authors.lastname: Klee
keywords: IL4-4; flavor; fruit ripening; metabolome; transcriptome