title: Measurement of Cortical Thickness and Volume of Subcortical Structures in Multiple Sclerosis: Agreement between 2D Spin-Echo and 3D MPRAGE T1-Weighted Images.
abstract: Gray matter pathology is known to occur in multiple sclerosis and is related to disease outcomes. FreeSurfer and the FMRIB Integrated Registration and Segmentation Tool (FIRST) have been developed for measuring cortical and subcortical gray matter in 3D-gradient-echo T1-weighted images. Unfortunately, most historical MS cohorts do not have 3D-gradient-echo, but 2D-spin-echo images instead. We aimed to evaluate whether cortical thickness and the volume of subcortical structures measured with FreeSurfer and FIRST could be reliably measured in 2D-spin-echo images and to investigate the strength and direction of clinicoradiologic correlations.
  
  
  
  Thirty-eight patients with MS and 2D-spin-echo and 3D-gradient-echo T1-weighted images obtained at the same time were analyzed by using FreeSurfer and FIRST. The intraclass correlation coefficient between the estimates was obtained. Correlation coefficients were used to investigate clinicoradiologic associations.
  
  
  
  Subcortical volumes obtained with both FreeSurfer and FIRST showed good agreement between 2D-spin-echo and 3D-gradient-echo images, with 68.8%-76.2% of the structures having either a substantial or almost perfect agreement. Nevertheless, with FIRST with 2D-spin-echo, 18% of patients had mis-segmentation. Cortical thickness had the lowest intraclass correlation coefficient values, with only 1 structure (1.4%) having substantial agreement. Disease duration and the Expanded Disability Status Scale showed a moderate correlation with most of the subcortical structures measured with 3D-gradient-echo images, but some correlations lost significance with 2D-spin-echo images, especially with FIRST.
  
  
  
  Cortical thickness estimates with FreeSurfer on 2D-spin-echo images are inaccurate. Subcortical volume estimates obtained with FreeSurfer and FIRST on 2D-spin-echo images seem to be reliable, with acceptable clinicoradiologic correlations for FreeSurfer.
authors.affiliation: From the Department of Neurology-Neuroimmunology and Multiple Sclerosis Centre of Catalonia (A.V.-J., J.S.-G., E.C., X.M.).
authors.forename: A
authors.identifier: 0000-0002-7270-5507
authors.initials: A
authors.lastname: Vidal-Jordana
authors.affiliation: Magnetic Resonance Unit (D.P., C.A., A.R.), Radiology Department, Hospital Universitari Vall d'Hebron, Universitat Autònoma de Barcelona, Barcelona, Spain. deborah.pareto@idi.gencat.cat.
authors.forename: D
authors.identifier: 0000-0001-7356-3769
authors.initials: D
authors.lastname: Pareto
authors.affiliation: From the Department of Neurology-Neuroimmunology and Multiple Sclerosis Centre of Catalonia (A.V.-J., J.S.-G., E.C., X.M.).
authors.forename: J
authors.identifier: 0000-0002-1589-2254
authors.initials: J
authors.lastname: Sastre-Garriga
authors.affiliation: Magnetic Resonance Unit (D.P., C.A., A.R.), Radiology Department, Hospital Universitari Vall d'Hebron, Universitat Autònoma de Barcelona, Barcelona, Spain.
authors.forename: C
authors.identifier: 0000-0001-9846-4767
authors.initials: C
authors.lastname: Auger
authors.affiliation: From the Department of Neurology-Neuroimmunology and Multiple Sclerosis Centre of Catalonia (A.V.-J., J.S.-G., E.C., X.M.).
authors.forename: E
authors.identifier: 0000-0002-7330-5433
authors.initials: E
authors.lastname: Ciampi
authors.affiliation: From the Department of Neurology-Neuroimmunology and Multiple Sclerosis Centre of Catalonia (A.V.-J., J.S.-G., E.C., X.M.).
authors.forename: X
authors.identifier: 0000-0002-0098-9918
authors.initials: X
authors.lastname: Montalban
authors.affiliation: Magnetic Resonance Unit (D.P., C.A., A.R.), Radiology Department, Hospital Universitari Vall d'Hebron, Universitat Autònoma de Barcelona, Barcelona, Spain.
authors.forename: A
authors.identifier: 0000-0002-2132-6750
authors.initials: A
authors.lastname: Rovira
mesh_terms: D000328:Adult; D002540:Cerebral Cortex / Q000000981:diagnostic imaging* / Q000473:pathology; D005260:Female; D066128:Gray Matter / Q000000981:diagnostic imaging* / Q000473:pathology; D006801:Humans; D021621:Imaging, Three-Dimensional / Q000379:methods*; D008279:Magnetic Resonance Imaging / Q000379:methods*; D008297:Male; D008875:Middle Aged; D009103:Multiple Sclerosis / Q000000981:diagnostic imaging* / Q000473:pathology; D059906:Neuroimaging / Q000379:methods*