title: Development of a fluorescence resonance energy transfer-based intracellular assay to identify novel enterovirus 71 antivirals.
abstract: Enterovirus 71 (EV71) is considered one of the most virulent pathogens in the family Picornaviridae. However, there have been no effective treatments for the severe complications caused by EV71. Development of new drugs against targets that are essential for viral replication often requires screening large collections of compounds, for which a high-throughput screening platform is needed. In this study, a drug-screening platform was developed based on a genetically engineered cell line that displays fluorescence resonance energy transfer (FRET) and shows a real-time and quantifiable impairment of FRET upon EV71 infection. A library of small molecules consisting of 1280 compounds with defined bioactivities was used for screening drugs with anti-EV71 activity; accurate, rapid, and robust results were obtained from this screening procedure. Ten drugs were identified in the primary screening, and their antiviral activities were indicated by dose-dependent elevation of FRET. Among these, AC-93253, mitoxantrone and N-bromoacetamide had not been reported as enterovirus inhibitors, and it was confirmed that they were able to suppress viral yields in a dose-dependent manner. Taken together, these studies demonstrate the feasibility of this FRET-based platform for efficient screening and identification of novel compounds with activity against EV71 infection.
authors.affiliation: Department of Clinical Pathology, Cheng Hsin General Hospital, Taipei, Taiwan, R.O.C.
authors.forename: Wen-Wen
authors.initials: WW
authors.lastname: Lu
authors.affiliation: Department of Biotechnology and Laboratory Science in Medicine, National Yang-Ming University, Taipei, Taiwan, R.O.C.
authors.forename: Fang-Yen
authors.initials: FY
authors.lastname: Kung
authors.affiliation: Department of Biotechnology and Laboratory Science in Medicine, National Yang-Ming University, Taipei, Taiwan, R.O.C.
authors.forename: Po-An
authors.initials: PA
authors.lastname: Deng
authors.affiliation: Department of Biotechnology and Laboratory Science in Medicine, National Yang-Ming University, Taipei, Taiwan, R.O.C.
authors.forename: Yu-Chu
authors.initials: YC
authors.lastname: Lin
authors.affiliation: Department of Medical Laboratory Science and Biotechnology, China Medical University, Taichung, Taiwan, R.O.C.
authors.forename: Cheng-Wen
authors.initials: CW
authors.lastname: Lin
authors.affiliation: Department of Biotechnology and Laboratory Science in Medicine, National Yang-Ming University, Taipei, Taiwan, R.O.C.. szkung@ym.edu.tw.
authors.forename: Szu-Hao
authors.identifier: http://orcid.org/0000-0002-0674-951X
authors.initials: SH
authors.lastname: Kung
mesh_terms: D000998:Antiviral Agents / Q000494:pharmacology*; D002460:Cell Line; D004353:Drug Evaluation, Preclinical / Q000379:methods*; D029821:Enterovirus A, Human / Q000187:drug effects* / Q000502:physiology; D004769:Enterovirus Infections / Q000821:virology*; D031541:Fluorescence Resonance Energy Transfer / Q000379:methods*; D006801:Humans; D054852:Small Molecule Libraries / Q000494:pharmacology*
chemical_list: D000998:Antiviral Agents; D054852:Small Molecule Libraries