title: Metabolic responses of Haliotis diversicolor to Vibrio parahaemolyticus infection.
abstract: Vibrio parahemolyticus is a devastating bacterial pathogen that often causes outbreak of vibriosis in abalone Haliotis diversicolor. Elucidation of metabolic mechanisms of abalones in responding to V. parahemolyticus infection is essential for controlling the epidemic. In this work,1H NMR-based metabolomic techniques along with correlation and network analyses are used to investigate characteristic metabolites, as well as corresponding disturbed pathways in hepatopancreas and gill of H. diversicolor after V. parahemolyticus infection for 48 h. Results indicate that obvious gender- and tissue-specific metabolic responses are induced. Metabolic responses in female abalones are more clearly observed than those in males, which are primarily manifested in the accumulation of branched-chain amino acids and the depletion of organic osmolytes (homarine, betaine and taurine) in the infected gills of female abalones, as well as in the depletion of glutamate, branched-chain and aromatic amino acids in the infected hepatopancreases of female abalones. Moreover, based on major metabolic functions of the characteristic metabolites, we have found that V. parahemolyticus infection not only cause the disturbance in energy metabolism, nucleotide metabolism and osmotic balance, but also induce oxidative stress, immune stress and neurotoxic effect in different tissues with various mechanisms. Our study provides details of metabolic responses of abalones to V. parahemolyticus infection and will shed light on biochemical defence mechanisms of male and female hosts against pathogen infection.
authors.affiliation: Department of Electronic Science, Fujian Provincial Key Laboratory of Plasma and Magnetic Resonance, Xiamen University, Xiamen 361005, PR China.
authors.forename: Jie
authors.initials: J
authors.lastname: Lu
authors.affiliation: Department of Chemical and Biochemical Engineering, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, PR China.
authors.forename: Yanyan
authors.initials: Y
authors.lastname: Shi
authors.affiliation: Department of Electronic Science, Fujian Provincial Key Laboratory of Plasma and Magnetic Resonance, Xiamen University, Xiamen 361005, PR China. Electronic address: shcai@xmu.edu.cn.
authors.forename: Shuhui
authors.initials: S
authors.lastname: Cai
authors.affiliation: Department of Electronic Science, Fujian Provincial Key Laboratory of Plasma and Magnetic Resonance, Xiamen University, Xiamen 361005, PR China. Electronic address: jianghua.feng@xmu.edu.cn.
authors.forename: Jianghua
authors.initials: J
authors.lastname: Feng
mesh_terms: D000818:Animals; D005260:Female; D049851:Gastropoda / Q000276:immunology / Q000378:metabolism* / Q000382:microbiology*; D007113:Immunity, Innate; D008297:Male; D055442:Metabolome; D009928:Organ Specificity; D066244:Proton Magnetic Resonance Spectroscopy; D011897:Random Allocation; D012737:Sex Factors; D014736:Vibrio parahaemolyticus / Q000502:physiology*
keywords: Gill; Haliotis diversicolor; Hepatopancreas; Metabolomics; Vibrio parahaemolyticus