title: Brevibacillus halotolerans sp. nov., isolated from saline soil of a paddy field.
abstract: Two novel aerobic bacteria, designated strains LAM0312T and LAM0313, were isolated from saline soil samples collected from a paddy field in Dezhou city, Shandong Province, China. Cells of these strains were Gram-stain-positive, sporogenous, rod-shaped and motile with peritrichous flagella. The optimal growth temperature and pH were 30 °C and pH 7.0-8.0. Strain LAM0312T was able to grow in the presence of 12 % (w/v) NaCl. The major fatty acids were anteiso-C15 : 0 and iso-C15 : 0. The dominant polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, glycolipids, five unidentitied lipids and phosphatidylmonomethylethanolamine. The cell-wall peptidoglycan was found to contain meso-diaminopimelic acid. The predominant menaquinone was identified as menaquinone-7. The G+C content of the genomic DNA of strains LAM0312T and LAM0313 was 45.0 and 46.0 mol%, respectively, as determined by the Tm method. 16S rRNA gene sequence similarity analysis indicated that the strains were closely related to Brevibacillus laterosporus DSM 25T and Brevibacillus fluminis JCM 15716T with 98.5 and 96.4 % sequence similarity, respectively. The DNA-DNA hybridization value between strain LAM0312T and LAM0313 was 92±0.6 % (reciprocal 90±0.2 %) and the value between strain LAM0312T and Brevibacillus laterosporus DSM 25T was 48±0.5 % (reciprocal 40±0.4 %). On the basis of the phenotypic, phylogenetic and chemotaxonomic characteristics, the two strains are proposed to represent a novel species of the genus Brevibacillus, for which the name Brevibacillus halotolerans sp. nov. is proposed. The type strain is LAM0312T (=ACCC 06527T=JCM 30849T).
authors.affiliation: Chinese Academy of Fishery Sciences, Beijing 100141, PR China.|Key Laboratory of Microbial Resources (Ministry of Agriculture, China), Institute of Agricultural Resources and Regional Planning, CAAS, Beijing 100081, PR China.
authors.forename: Jinlong
authors.initials: J
authors.lastname: Song
authors.affiliation: Key Laboratory of Development and Application of Rural Renewable Energy (Ministry of Agriculture), Biogas Institute of Ministry of Agriculture, Chengdu 610041, PR China.
authors.forename: Yanwei
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Chinese Academy of Fishery Sciences, Beijing 100141, PR China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Song
authors.affiliation: Key Laboratory of Microbial Resources (Ministry of Agriculture, China), Institute of Agricultural Resources and Regional Planning, CAAS, Beijing 100081, PR China.
authors.forename: Bingqiang
authors.initials: B
authors.lastname: Zhao
authors.affiliation: Key Laboratory of Microbial Resources (Ministry of Agriculture, China), Institute of Agricultural Resources and Regional Planning, CAAS, Beijing 100081, PR China.
authors.forename: Huimin
authors.initials: H
authors.lastname: Wang
authors.affiliation: Key Laboratory of Microbial Resources (Ministry of Agriculture, China), Institute of Agricultural Resources and Regional Planning, CAAS, Beijing 100081, PR China.
authors.forename: Shan
authors.initials: S
authors.lastname: Zhou
authors.affiliation: Key Laboratory of Microbial Resources (Ministry of Agriculture, China), Institute of Agricultural Resources and Regional Planning, CAAS, Beijing 100081, PR China.
authors.forename: Delong
authors.initials: D
authors.lastname: Kong
authors.affiliation: Key Laboratory of Development and Application of Rural Renewable Energy (Ministry of Agriculture), Biogas Institute of Ministry of Agriculture, Chengdu 610041, PR China.
authors.forename: Xiang
authors.initials: X
authors.lastname: Guo
authors.affiliation: Key Laboratory of Microbial Resources (Ministry of Agriculture, China), Institute of Agricultural Resources and Regional Planning, CAAS, Beijing 100081, PR China.
authors.forename: Yanting
authors.initials: Y
authors.lastname: Li
authors.affiliation: Key Laboratory of Development and Application of Rural Renewable Energy (Ministry of Agriculture), Biogas Institute of Ministry of Agriculture, Chengdu 610041, PR China.
authors.forename: Mingxiong
authors.initials: M
authors.lastname: He
authors.affiliation: College of Environmental and Chemical Engineering, Dalian University, Dalian 116622, PR China.
authors.forename: Kedong
authors.initials: K
authors.lastname: Ma
authors.affiliation: Key Laboratory of Microbial Resources (Ministry of Agriculture, China), Institute of Agricultural Resources and Regional Planning, CAAS, Beijing 100081, PR China.
authors.forename: Zhiyong
authors.initials: Z
authors.lastname: Ruan
authors.affiliation: Graduate School, Chinese Academy of Agricultural Sciences, Beijing 100081, PR China.
authors.forename: Yanchun
authors.initials: Y
authors.lastname: Yan
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D058675:Brevibacillus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D002681:China; D004269:DNA, Bacterial / Q000235:genetics; D003960:Diaminopimelic Acid / Q000737:chemistry; D005227:Fatty Acids / Q000737:chemistry; D006017:Glycolipids / Q000737:chemistry; D009693:Nucleic Acid Hybridization; D012275:Oryza; D010457:Peptidoglycan / Q000737:chemistry; D010743:Phospholipids / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D054712:Salinity; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D006017:Glycolipids; D010457:Peptidoglycan; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; D003960:Diaminopimelic Acid; C062629:menaquinone 7