title: Highly expressed ribosomal protein L34 indicates poor prognosis in osteosarcoma and its knockdown suppresses osteosarcoma proliferation probably through translational control.
abstract: Osteosarcoma has devastating health implications on children and adolescents. However, due to its low incidence and high tumor heterogeneity, it is hard to achieve any further improvements in therapy and overall survival. Ribosomal protein L34 (RPL34) has been increasingly recognized to promote the proliferation of malignant cells, but its role in osteosarcoma has not been investigated. In this study, real-time quantitative PCR (RT-qPCR) and immunohistochemistry revealed that RPL34 was highly expressed in osteosarcoma tissues when compared to adjacent tissues and normal bone tissues. Survival analysis showed that high expression of RPL34 predicted a poor prognosis for osteosarcoma patients. Knockdown of RPL34 in Saos-2 cells via lentivirus-mediated small interfering RNA (siRNA) significantly inhibited cell proliferation, induced cell apoptosis and G2/M phase arrest. Moreover, screening of transcription factors using University of California Santa Cruz (UCSC) Genome Browser, protein-protein interaction (PPI) network analysis, Gene Ontology (GO) and pathway enrichment analysis revealed that MYC participates in the transcriptional regulation of RPL34, which interacts with the subunits of eukaryotic translation initiation factor 3 (eIF3) and probably involves the translational control of growth-promoting proteins. Our findings suggest that RPL34 plays an important role in the proliferation of osteosarcoma cells.
authors.affiliation: Division of Spinal Surgery, The First Affiliated Hospital of Guangxi Medical University, No. 6 Shuangyong Road, Nanning, Guangxi, 530021, China.|Guangxi Key Laboratory of Regenerative Medicine, Guangxi Medical University, No. 22 Shuangyong Road, Nanning, Guangxi, 530021, China.
authors.forename: Shuju
authors.initials: S
authors.lastname: Luo
authors.affiliation: Guangxi Key Laboratory of Regenerative Medicine, Guangxi Medical University, No. 22 Shuangyong Road, Nanning, Guangxi, 530021, China.|Department of Orthopedic Trauma and Hand Surgery, The First Affiliated Hospital of Guangxi, Medical University, No. 6 Shuangyong Road, Nanning, Guangxi, 530021, China.
authors.forename: Jinmin
authors.initials: J
authors.lastname: Zhao
authors.affiliation: Division of Spinal Surgery, The First Affiliated Hospital of Guangxi Medical University, No. 6 Shuangyong Road, Nanning, Guangxi, 530021, China.|Guangxi Key Laboratory of Regenerative Medicine, Guangxi Medical University, No. 22 Shuangyong Road, Nanning, Guangxi, 530021, China.
authors.forename: Mitra
authors.initials: M
authors.lastname: Fowdur
authors.affiliation: Division of Spinal Surgery, The First Affiliated Hospital of Guangxi Medical University, No. 6 Shuangyong Road, Nanning, Guangxi, 530021, China.|Guangxi Key Laboratory of Regenerative Medicine, Guangxi Medical University, No. 22 Shuangyong Road, Nanning, Guangxi, 530021, China.
authors.forename: Kun
authors.initials: K
authors.lastname: Wang
authors.affiliation: Division of Spinal Surgery, The First Affiliated Hospital of Guangxi Medical University, No. 6 Shuangyong Road, Nanning, Guangxi, 530021, China.|Guangxi Key Laboratory of Regenerative Medicine, Guangxi Medical University, No. 22 Shuangyong Road, Nanning, Guangxi, 530021, China.
authors.forename: Tenglong
authors.initials: T
authors.lastname: Jiang
authors.affiliation: Division of Spinal Surgery, The First Affiliated Hospital of Guangxi Medical University, No. 6 Shuangyong Road, Nanning, Guangxi, 530021, China.|Guangxi Key Laboratory of Regenerative Medicine, Guangxi Medical University, No. 22 Shuangyong Road, Nanning, Guangxi, 530021, China.
authors.forename: Maolin
authors.initials: M
authors.lastname: He
mesh_terms: D017209:Apoptosis / Q000235:genetics; D059447:Cell Cycle Checkpoints / Q000235:genetics; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000235:genetics; D015972:Gene Expression Regulation, Neoplastic; D055785:Gene Knockdown Techniques; D063990:Gene Ontology; D006801:Humans; D007150:Immunohistochemistry; D053208:Kaplan-Meier Estimate; D016086:Lentivirus / Q000378:metabolism; D012516:Osteosarcoma / Q000235:genetics* / Q000473:pathology*; D011379:Prognosis; D014176:Protein Biosynthesis; D025941:Protein Interaction Mapping; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D034741:RNA, Small Interfering / Q000378:metabolism; D012269:Ribosomal Proteins / Q000235:genetics / Q000378:metabolism*; D014157:Transcription Factors / Q000378:metabolism; D016896:Treatment Outcome; D014410:Tumor Stem Cell Assay; D015854:Up-Regulation / Q000235:genetics
chemical_list: D012333:RNA, Messenger; D034741:RNA, Small Interfering; D012269:Ribosomal Proteins; D014157:Transcription Factors; C041672:ribosomal protein L34