title: Construction of Small-Insert and Large-Insert Metagenomic Libraries.
abstract: The vast majority of the Earth's biological diversity is hidden in uncultured and yet uncharacterized microbial genomes. The construction of metagenomic libraries is a cultivation-independent molecular approach to assess this unexplored genetic reservoir. High numbers of novel biocatalysts have been identified by function-based or sequence-based screening of metagenomic libraries derived from various environments. Here, we describe detailed protocols for the construction of metagenomic small-insert and large-insert libraries in plasmids and fosmids, respectively, from environmental DNA.
authors.affiliation: Pohl-Boskamp GmbH, Hamburg, Germany.
authors.forename: Carola
authors.initials: C
authors.lastname: Simon
authors.affiliation: Göttingen Genomics Laboratory, Department of Genomic and Applied Microbiology, Institut für Mikrobiologie und Genetik, Georg-August-Universität Göttingen, Grisebachstr. 8, 37077, Göttingen, Germany. rdaniel@gwdg.de.
authors.forename: Rolf
authors.initials: R
authors.lastname: Daniel
mesh_terms: D004783:Environmental Microbiology; D015723:Gene Library; D005818:Genetic Engineering / Q000379:methods; D005822:Genetic Vectors / Q000235:genetics; D054892:Metagenome; D056186:Metagenomics / Q000379:methods; D016254:Mutagenesis, Insertional; D010957:Plasmids / Q000235:genetics
keywords: Fosmid; Large-insert library; Metagenomic DNA; Plasmid; Small-insert library; WGA; Whole genome amplification