title: G2691A and C2491T mutations of factor V gene and pre-disposition to myocardial infarction in Morocco.
abstract: Coagulation factor Leiden mutation has been described as a common genetic risk factor for venous thrombosis; however, this mutation was reported to be practically absent in an African population. Recently, a novel non-sense mutation in the gene encoding factor V has been associated with the risk of occurrence of cardio-cerebrovascular diseases such as stroke and venous thrombosis. The aim of the present study was to investigate whether the factor V Leiden (FVL) and C2491T non-sense mutations are associated with the risk of developing myocardial infarction. Genotyping of FVL and C2491T FV was performed using the polymerase chain reaction restriction fragment length polymorphism method on a sample of 100 patients with myocardial infarction as well as 211 controls. In the study population, the frequency of the FVL mutation was practically zero. However, with regard to the C2491T mutation, the TT genotype was associated with an increased risk of myocardial infarction [odds ratio (OR)=3.16, 95% confidence interval (CI): 1.29-7.71, P=0.03]. A significant association between the C2491T FV mutation and the risk of myocardial infarction was identified using recessive (OR=2.74, 95% CI: 1.14-6.58, P=0.04), dominant (OR=1.85, 95% CI: 1.13-3.04, P=0.02) and additive (OR=1.88, 95% CI: 1.25-2.80, P=0.004) models. Furthermore, a positive correlation was found between the presence of the C2491T FV mutation and hypertension (P=0.02), which is associated with myocardial infarction. In conclusion, the results of the present study suggested that the C2491T non-sense mutation of the FV gene may be a risk factor for myocardial infarction in a Moroccan population.
authors.affiliation: Laboratory of Genetics and Molecular Pathology, Medical School, University Hassan II, Casablanca 22000, Morocco.
authors.forename: Wiam
authors.initials: W
authors.lastname: Hmimech
authors.affiliation: Laboratory of Genetics and Molecular Pathology, Medical School, University Hassan II, Casablanca 22000, Morocco.
authors.forename: Brehima
authors.initials: B
authors.lastname: Diakite
authors.affiliation: Laboratory of Genetics and Molecular Pathology, Medical School, University Hassan II, Casablanca 22000, Morocco.
authors.forename: Hind Hassani
authors.initials: HH
authors.lastname: Idrissi
authors.affiliation: Laboratory of Genetics and Molecular Pathology, Medical School, University Hassan II, Casablanca 22000, Morocco.
authors.forename: Khalil
authors.initials: K
authors.lastname: Hamzi
authors.affiliation: Department of Cardiology, University Hospital Center Ibn Rochd, Casablanca 22000, Morocco.
authors.forename: Farah
authors.initials: F
authors.lastname: Korchi
authors.affiliation: Department of Cardiology, University Hospital Center Ibn Rochd, Casablanca 22000, Morocco.
authors.forename: Dalila
authors.initials: D
authors.lastname: Baghdadi
authors.affiliation: Department of Cardiology, University Hospital Center Ibn Rochd, Casablanca 22000, Morocco.
authors.forename: Rachida
authors.initials: R
authors.lastname: Habbal
authors.affiliation: Laboratory of Genetics and Molecular Pathology, Medical School, University Hassan II, Casablanca 22000, Morocco.
authors.forename: Sellama
authors.initials: S
authors.lastname: Nadifi
keywords: C2491T; factor V Leiden; mutation; myocardial infarction; non-sense