title: Artifacts Arising from Using Leukocytic Fc Receptor Blocking Buffer.
abstract: We studied the effects of Human TruStain FcX buffer (Fcγ receptor blocking solution) in experiments on evaluation of TLR4 level with labeled monoclonal antibodies, intracellular immunofluorescent staining of NF-κB p50, and TNF-α synthesis on human isolated monocytes and whole blood cells. The influence of the blocking buffer on the measured parameters should be taken into account and appropriateness of its use in experiments on isolated cells and whole blood should be considered.
authors.affiliation: Institute of Fundamental Problems of Biology, Russian Academy of Sciences, Pushchino, Russia. zusvet@rambler.ru.
authors.forename: S V
authors.initials: SV
authors.lastname: Zubova
authors.affiliation: Institute of Fundamental Problems of Biology, Russian Academy of Sciences, Pushchino, Russia.
authors.forename: D S
authors.initials: DS
authors.lastname: Kabanov
authors.affiliation: Institute of Fundamental Problems of Biology, Russian Academy of Sciences, Pushchino, Russia.
authors.forename: D A
authors.initials: DA
authors.lastname: Serov
authors.affiliation: Institute of Fundamental Problems of Biology, Russian Academy of Sciences, Pushchino, Russia.
authors.forename: S V
authors.initials: SV
authors.lastname: Grachev
authors.affiliation: Institute of Fundamental Problems of Biology, Russian Academy of Sciences, Pushchino, Russia.
authors.forename: I R
authors.initials: IR
authors.lastname: Prokhorenko
mesh_terms: D000911:Antibodies, Monoclonal / Q000737:chemistry / Q000276:immunology; D016477:Artifacts; D001773:Blood Cells / Q000276:immunology / Q000378:metabolism; D002021:Buffers; D004797:Enzyme-Linked Immunosorbent Assay / Q000592:standards; D005434:Flow Cytometry / Q000592:standards; D015870:Gene Expression; D006801:Humans; D007074:Immunoglobulin G / Q000737:chemistry / Q000276:immunology; D007202:Indicators and Reagents / Q000737:chemistry; D009000:Monocytes / Q000276:immunology / Q000378:metabolism*; D052002:NF-kappa B p50 Subunit / Q000032:analysis* / Q000235:genetics / Q000276:immunology; D061251:Primary Cell Culture; D011961:Receptors, Fc / Q000037:antagonists & inhibitors* / Q000235:genetics / Q000276:immunology; D051197:Toll-Like Receptor 4 / Q000032:analysis* / Q000235:genetics / Q000276:immunology; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis* / Q000235:genetics / Q000276:immunology
chemical_list: D000911:Antibodies, Monoclonal; D002021:Buffers; D007074:Immunoglobulin G; D007202:Indicators and Reagents; D052002:NF-kappa B p50 Subunit; D011961:Receptors, Fc; C495345:TLR4 protein, human; D051197:Toll-Like Receptor 4; D014409:Tumor Necrosis Factor-alpha
keywords: Fcγ receptors; HTA125; Human TruStain FcX; NF-κB p50; TNF-α