title: Biorefinery of microalgal soluble proteins by sequential processing and membrane filtration.
abstract: A mild biorefinery process was investigated on the microalga Nannochloropsis gaditana, to obtain an enriched fraction of water soluble proteins free from chlorophyll. After harvesting, a 100g.L-1solution of cells was first subjected to cell disruption by either high-pressure homogenization (HPH) or enzymatic treatment (ENZ). HPH resulted in a larger release of proteins (49%) in the aqueous phase compared to the Alcalase incubation (35%). In both cases, an ultrafiltration/diafiltration (UF/DF) was then performed on the supernatant obtained from cell disruption by testing different membrane cut-off (1000kDa, 500kDa and 300kDa). After optimising the process conditions, the combination of ENZ→UF/DF ended in a larger overall yield of water soluble proteins (24.8%) in the permeate compared to the combination of HPH→UF/DF (17.4%). A gel polarization model was implemented to assess the maximum achievable concentration factor during ultrafiltration and the mass transfer coefficient related to the theoretical permeation flux rate.
authors.affiliation: Wageningen Food & Biobased Research, AlgaePARC, PO Box 17, 6700 AA Wageningen, The Netherlands. Electronic address: carl.safi@wur.nl.
authors.forename: C
authors.initials: C
authors.lastname: Safi
authors.affiliation: Bioprocess Engineering, AlgaePARC, Wageningen University, PO Box 16, 6700 AA Wageningen, The Netherlands; Dipartimento di Ingegneria Chimica, dei Materiali e della Produzione Industriale, Università degli Studi di Napoli Federico II, Piazzale V. Tecchio 80, 80125 Napoli, Italy.
authors.forename: G
authors.initials: G
authors.lastname: Olivieri
authors.affiliation: Wageningen Food & Biobased Research, AlgaePARC, PO Box 17, 6700 AA Wageningen, The Netherlands.
authors.forename: R P
authors.initials: RP
authors.lastname: Campos
authors.affiliation: Wageningen Food & Biobased Research, AlgaePARC, PO Box 17, 6700 AA Wageningen, The Netherlands.
authors.forename: N
authors.initials: N
authors.lastname: Engelen-Smit
authors.affiliation: Wageningen Food & Biobased Research, AlgaePARC, PO Box 17, 6700 AA Wageningen, The Netherlands.
authors.forename: W J
authors.initials: WJ
authors.lastname: Mulder
authors.affiliation: Wageningen Food & Biobased Research, AlgaePARC, PO Box 17, 6700 AA Wageningen, The Netherlands.
authors.forename: L A M
authors.initials: LAM
authors.lastname: van den Broek
authors.affiliation: Wageningen Food & Biobased Research, AlgaePARC, PO Box 17, 6700 AA Wageningen, The Netherlands.
authors.forename: L
authors.initials: L
authors.lastname: Sijtsma
mesh_terms: D002734:Chlorophyll / Q000737:chemistry; D008567:Membranes, Artificial; D058086:Microalgae / Q000737:chemistry*; D011134:Polysaccharides / Q000737:chemistry; D011312:Pressure; D011506:Proteins / Q000302:isolation & purification*; D012995:Solubility; D058009:Stramenopiles / Q000737:chemistry*; D013381:Subtilisins / Q000737:chemistry; D014462:Ultrafiltration / Q000295:instrumentation / Q000379:methods*; D014867:Water
chemical_list: D008567:Membranes, Artificial; D011134:Polysaccharides; D011506:Proteins; D014867:Water; D002734:Chlorophyll; D013381:Subtilisins
keywords: Biorefinery; Cell disruption; Fractionation; Proteins; Ultrafiltration