title: Morphometric analysis of the folliculostellate cells and luteinizing hormone gonadotropic cells of the anterior pituitary of the men during the aging process.
abstract: The aim of this research was to quantify the changes in the morphology and density of the anterior pituitary folliculostellate (FS) and luteinizing hormone (LH) cells. Material was tissue of the pituitary gland of the 14 male cadavers. Tissue slices were immunohistochemically stained with monoclonal anti-LH antibody and polyclonal anti-S100 antibody for the detection of LH and FS cells, respectively. Digital images of the stained slices were afterwards morphometrically analyzed by ImageJ. Results of the morphometric analysis showed significant increase of the FS cells volume density in cases older than 70 years. Volume density of the LH cells did not significantly change, whereas their area significantly increased with age. Nucleocytoplasmic ratio of the LH cells gradually decreased and became significant after the age of 70. Finally, volume density of the FS cell significantly correlated with LH cells area and nucleocytoplasmic ratio. From all above cited, we concluded that in men, density and size of the FS cells increase with age. Long-term hypertrophy of the LH cells results in their functional decline after the age of 70. Strong correlation between FS cells and LH cells morphometric parameters might point to age-related interaction between these two cell groups.
authors.affiliation: Department of Anatomy, Faculty of Medicine, University of Niš, Serbia.
authors.forename: Jovana
authors.initials: J
authors.lastname: Čukuranović Kokoris
authors.affiliation: Department of Anatomy, Faculty of Medicine, University of Niš, Serbia. Electronic address: ivanjov@sbb.rs.
authors.forename: Ivan
authors.initials: I
authors.lastname: Jovanović
authors.affiliation: Public Health Institute Niš, Niš, Serbia.
authors.forename: Vukica
authors.initials: V
authors.lastname: Pantović
authors.affiliation: Institute of Pathology, Faculty of Medicine, University of Niš, Niš, Serbia.
authors.forename: Miljan
authors.initials: M
authors.lastname: Krstić
authors.affiliation: Institute of Forensic Medicine, Faculty of Medicine, University of Niš, Niš, Serbia.
authors.forename: Milica
authors.initials: M
authors.lastname: Stanojković
authors.affiliation: University of Belgrade, Institute for Biochemical Research "Siniša Stanković", Beograd, Serbia.
authors.forename: Verica
authors.initials: V
authors.lastname: Milošević
authors.affiliation: Department of Anatomy, Faculty of Medicine, University of Niš, Serbia.
authors.forename: Slađana
authors.initials: S
authors.lastname: Ugrenović
authors.affiliation: Department of Anatomy, Faculty of Medicine, University of Niš, Serbia.
authors.forename: Vesna
authors.initials: V
authors.lastname: Stojanović
mesh_terms: D000328:Adult; D000367:Age Factors; D000368:Aged; D000369:Aged, 80 and over; D000375:Aging / Q000378:metabolism* / Q000473:pathology; D002102:Cadaver; D002452:Cell Count; D048429:Cell Size; D005260:Female; D005640:Follicle Stimulating Hormone / Q000378:metabolism*; D006801:Humans; D008184:Luteal Cells / Q000378:metabolism; D007986:Luteinizing Hormone / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D010903:Pituitary Gland, Anterior / Q000378:metabolism* / Q000473:pathology
chemical_list: D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone
keywords: Aging; Folliculostellate cells; Gonadotropes; Morphometry