title: Development and Validation of an LC-ESI-MS/MS Method for Simultaneous Determination of Ligustroflavone and Rhoifolin in Rat Plasma and Its Application to a Pharmacokinetic Study.
abstract: A selective and sensitive liquid chromatography-electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS) method was developed for the simultaneous determination of ligustroflavone and rhoifolin in rat plasma. Chromatographic separation was performed on a Venusil HILIC column using an isocratic mobile phase consisting of acetonitrile/water/formic acid (75:25:0.1, v/v/v). The detection was achieved using a triple-quadrupole tandem MS in negative ionization through selected reaction monitoring (SRM) mode. The calibration curves of both analytes in plasma showed good linearity over the concentration ranges of 3-300 ng/mL for ligustroflavone, and 2-200 ng/mL for rhoifolin. This assay was used to investigate the pharmacokinetics of ligustroflavone and rhoifolin in rats.
authors.affiliation: Department of Infectious Disease, East Medical District of Linyi People's Hospital, Linyi 276000, PR China.
authors.forename: Hong
authors.initials: H
authors.lastname: Liu
authors.affiliation: Department of Infectious Disease, Linyi People's Hospital, Linyi 276000, PR China.
authors.forename: Cheng
authors.initials: C
authors.lastname: Xu
authors.affiliation: Traditional Chinese Medicine Department of Digestion, China-Japan Friendship Hospital, Beijing 100029, PR China.
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.affiliation: School of Postgraduate, Dalian Medical University, Dalian 116023, PR China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Zhao
mesh_terms: D000818:Animals; D047310:Apigenin / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics; D002853:Chromatography, Liquid / Q000379:methods*; D004187:Disaccharides / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics; D005419:Flavonoids / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics; D006027:Glycosides / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics; D016014:Linear Models; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D015203:Reproducibility of Results; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D053719:Tandem Mass Spectrometry / Q000379:methods*; D005947:Glucose / Q000031:analogs & derivatives; D008358:Mannose / Q000031:analogs & derivatives
chemical_list: D047310:Apigenin; D004187:Disaccharides; D005419:Flavonoids; D006027:Glycosides; C405585:ligustroflavone; C089378:rhoifolin; D005947:Glucose; D008358:Mannose