title: The Use of Technetium-99m for Intravital Tracing of Transplanted Multipotent Stromal Cells.
abstract: We studied the possibility of in vivo tracing of multipotent mesenchymal stromal cells labeled with a radiophermaceutic preparation based on metastable isotope Technetium-99m and injected to rats with modeled traumatic brain injury. Accumulation of labeled cells occurred primarily in the liver and lungs. The cells distribution in internal organs greatly varied depending on the administration route. Cell injection into the carotid artery led to their significant accumulation in the damaged brain hemisphere, while intravenous injection was followed by diffuse cell distribution in all brain structures. Scintigraphy data were confirmed by magnetic resonance imaging and histological staining of cells. Visualization of stem cells labeled with Technetium-99m-based preparation by scintigraphy is an objective and highly informative method allowing real-time in vivo cell tracing in the body.
authors.affiliation: V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation, Moscow, Russia.|A. N. Belozersky Institute of Physico-Chemical Biology, M. V. Lomonosov Moscow State University, Moscow, Russia.
authors.forename: D N
authors.initials: DN
authors.lastname: Silachev
authors.affiliation: N. I. Pirogov National Russian Research Medical University, Ministry of Health of the Russian Federation, Moscow, Russia.|Hospital for Incurable Patients - Research Medical Rehabilitation Center, Moscow, Russia.
authors.forename: A K
authors.initials: AK
authors.lastname: Kondakov
authors.affiliation: N. I. Pirogov National Russian Research Medical University, Ministry of Health of the Russian Federation, Moscow, Russia.|Hospital for Incurable Patients - Research Medical Rehabilitation Center, Moscow, Russia.
authors.forename: I A
authors.initials: IA
authors.lastname: Znamenskii
authors.affiliation: National Research Nuclear University MEPhI, Moscow, Russia.|P. A. Hertsen Moscow Oncology Research Institute, Moscow, Russia.
authors.forename: Yu B
authors.initials: YB
authors.lastname: Kurashvili
authors.affiliation: P. A. Hertsen Moscow Oncology Research Institute, Moscow, Russia.
authors.forename: A V
authors.initials: AV
authors.lastname: Abolenskaya
authors.affiliation: P. A. Hertsen Moscow Oncology Research Institute, Moscow, Russia.
authors.forename: N R
authors.initials: NR
authors.lastname: Antipkin
authors.affiliation: A. N. Belozersky Institute of Physico-Chemical Biology, M. V. Lomonosov Moscow State University, Moscow, Russia.
authors.forename: T I
authors.initials: TI
authors.lastname: Danilina
authors.affiliation: A. N. Belozersky Institute of Physico-Chemical Biology, M. V. Lomonosov Moscow State University, Moscow, Russia.
authors.forename: V N
authors.initials: VN
authors.lastname: Manskikh
authors.affiliation: Laboratory of Magnetic Tomography and Spectroscopy, Faculty of Fundamental Medicine, M. V. Lomonosov Moscow State University, Moscow, Russia.
authors.forename: M V
authors.initials: MV
authors.lastname: Gulyaev
authors.affiliation: Laboratory of Magnetic Tomography and Spectroscopy, Faculty of Fundamental Medicine, M. V. Lomonosov Moscow State University, Moscow, Russia.
authors.forename: Yu A
authors.initials: YA
authors.lastname: Pirogov
authors.affiliation: V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation, Moscow, Russia.|A. N. Belozersky Institute of Physico-Chemical Biology, M. V. Lomonosov Moscow State University, Moscow, Russia.
authors.forename: E Yu
authors.initials: EY
authors.lastname: Plotnikov
authors.affiliation: V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation, Moscow, Russia. zorov@genebee.msu.ru.|A. N. Belozersky Institute of Physico-Chemical Biology, M. V. Lomonosov Moscow State University, Moscow, Russia. zorov@genebee.msu.ru.
authors.forename: D B
authors.initials: DB
authors.lastname: Zorov
authors.affiliation: V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation, Moscow, Russia.
authors.forename: G T
authors.initials: GT
authors.lastname: Sukhikh
mesh_terms: D000818:Animals; D021461:Animals, Outbred Strains; D000070642:Brain Injuries, Traumatic / Q000000981:diagnostic imaging* / Q000378:metabolism / Q000473:pathology; D058948:Cell Tracking / Q000379:methods; D005290:Ferric Compounds / Q000737:chemistry; D007269:Injections, Intra-Arterial; D007275:Injections, Intravenous; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000737:chemistry / Q000166:cytology*; D010091:Oximes / Q000737:chemistry; D061251:Primary Cell Culture; D011877:Radionuclide Imaging / Q000379:methods*; D019275:Radiopharmaceuticals / Q000737:chemistry* / Q000378:metabolism / Q000493:pharmacokinetics; D051381:Rats; D013194:Staining and Labeling / Q000379:methods; D013667:Technetium / Q000737:chemistry* / Q000378:metabolism / Q000493:pharmacokinetics
chemical_list: D005290:Ferric Compounds; D010091:Oximes; D019275:Radiopharmaceuticals; C068296:hexamethylpropyleneamine oxime; C000499:ferric oxide; D013667:Technetium
keywords: brain; imaging; mesenchymal stem cells; scintigraphy; tomography