title: Structural characterization of EasH (Aspergillus japonicus) - an oxidase involved in cycloclavine biosynthesis.
abstract: Aj_EasH is a non-heme iron- and α-keto-glutarate-dependent oxidase that is responsible for an unusual cyclopropyl ring formation in the biosynthesis of the fungal ergot alkaloid cycloclavine. The three dimensional structure of Aj_EasH (2.2 Å resolution) reported here provides insight into the mechanism of this unusual and complex reaction.
authors.affiliation: Department of Biological Chemistry, John Innes Centre, Norwich Research Park, Norwich NR4 7UH, UK. Sarah.oconnor@jic.ac.uk.
authors.forename: Dorota
authors.initials: D
authors.lastname: Jakubczyk
authors.affiliation: Department of Biological Chemistry, John Innes Centre, Norwich Research Park, Norwich NR4 7UH, UK. Sarah.oconnor@jic.ac.uk.
authors.forename: Lorenzo
authors.initials: L
authors.lastname: Caputi
authors.affiliation: Department of Biological Chemistry, John Innes Centre, Norwich Research Park, Norwich NR4 7UH, UK. Sarah.oconnor@jic.ac.uk.
authors.forename: Clare E M
authors.initials: CE
authors.lastname: Stevenson
authors.affiliation: Department of Biological Chemistry, John Innes Centre, Norwich Research Park, Norwich NR4 7UH, UK. Sarah.oconnor@jic.ac.uk.
authors.forename: David M
authors.initials: DM
authors.lastname: Lawson
authors.affiliation: Department of Biological Chemistry, John Innes Centre, Norwich Research Park, Norwich NR4 7UH, UK. Sarah.oconnor@jic.ac.uk.
authors.forename: Sarah E
authors.initials: SE
authors.lastname: O'Connor
mesh_terms: D001230:Aspergillus / Q000201:enzymology*; D055162:Biocatalysis; D026121:Indole Alkaloids / Q000737:chemistry / Q000378:metabolism*; D008958:Models, Molecular; D010084:Oxidation-Reduction; D010088:Oxidoreductases / Q000737:chemistry* / Q000378:metabolism; D011487:Protein Conformation
chemical_list: D026121:Indole Alkaloids; C560517:cycloclavine; D010088:Oxidoreductases