title: Isolation of Bovine Skin-Derived Precursor Cells and Their Developmental Potential After Nuclear Transfer.
abstract: Nuclei from less differentiated stem cells yield high cloning efficiency. However, pluripotent stem cells are rather difficult to obtain from bovines. Skin-derived precursor (SKPs) cells exhibit a certain degree of pluripotency, which has been shown to enhance the efficiency of nuclear transfer (NT) in pigs. In this study, bovine SKPs were isolated and characterized. Results showed that bovine SKPs expressed nestin, fibronectin, vimentin, pluripotency-related genes, and characteristic neural crest markers, such as NGFR, PAX3, SOX9, SNAI2, and OCT4. Bovine SKPs and fibroblasts were used as NT donor cells to examine and compare the preimplantation developmental potential of reconstructed embryos after somatic cell nuclear transfer (SCNT). Bovine SKP-cloned embryos displayed higher developmental competence in terms of blastocyst formation rate and total cell number in blastocysts compared with the bovine embryonic fibroblast-cloned embryos. This study revealed that bovine SKPs may be considered excellent candidate nuclear donors for SCNT and may provide a promising platform for transgenic cattle generation.
authors.affiliation: 1 College of Veterinary Medicine, Northwest A&F University , Yangling, Shaanxi, 712100 China .|2 Key Laboratory of Animal Biotechnology, Ministry of Agriculture, Northwest A&F University , Yangling, Shaanxi, 712100 China .
authors.forename: Jiajia
authors.initials: J
authors.lastname: Xiao
authors.affiliation: 1 College of Veterinary Medicine, Northwest A&F University , Yangling, Shaanxi, 712100 China .|2 Key Laboratory of Animal Biotechnology, Ministry of Agriculture, Northwest A&F University , Yangling, Shaanxi, 712100 China .
authors.forename: Qiaoqiao
authors.initials: Q
authors.lastname: Li
authors.affiliation: 1 College of Veterinary Medicine, Northwest A&F University , Yangling, Shaanxi, 712100 China .|2 Key Laboratory of Animal Biotechnology, Ministry of Agriculture, Northwest A&F University , Yangling, Shaanxi, 712100 China .
authors.forename: Pengxiang
authors.initials: P
authors.lastname: Qu
authors.affiliation: 1 College of Veterinary Medicine, Northwest A&F University , Yangling, Shaanxi, 712100 China .|2 Key Laboratory of Animal Biotechnology, Ministry of Agriculture, Northwest A&F University , Yangling, Shaanxi, 712100 China .
authors.forename: Zihan
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: 1 College of Veterinary Medicine, Northwest A&F University , Yangling, Shaanxi, 712100 China .|2 Key Laboratory of Animal Biotechnology, Ministry of Agriculture, Northwest A&F University , Yangling, Shaanxi, 712100 China .
authors.forename: Shaohui
authors.initials: S
authors.lastname: Pan
authors.affiliation: 1 College of Veterinary Medicine, Northwest A&F University , Yangling, Shaanxi, 712100 China .|2 Key Laboratory of Animal Biotechnology, Ministry of Agriculture, Northwest A&F University , Yangling, Shaanxi, 712100 China .
authors.forename: Yongsheng
authors.initials: Y
authors.lastname: Wang
authors.affiliation: 1 College of Veterinary Medicine, Northwest A&F University , Yangling, Shaanxi, 712100 China .|2 Key Laboratory of Animal Biotechnology, Ministry of Agriculture, Northwest A&F University , Yangling, Shaanxi, 712100 China .
authors.forename: Yong
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D000818:Animals; D001755:Blastocyst / Q000166:cytology* / Q000502:physiology; D002417:Cattle; D002454:Cell Differentiation; D002469:Cell Separation; D002478:Cells, Cultured; D004622:Embryo, Mammalian / Q000166:cytology* / Q000502:physiology; D047108:Embryonic Development; D005260:Female; D005347:Fibroblasts / Q000166:cytology* / Q000502:physiology; D053652:Nuclear Transfer Techniques / Q000662:veterinary*; D039904:Pluripotent Stem Cells / Q000166:cytology* / Q000502:physiology; D012867:Skin / Q000166:cytology*
keywords: bovine; developmental competence; nuclear transfer; skin-derived precursor cells