title: Amelioration of energy metabolism by melatonin in skeletal muscle of rats with LPS induced endotoxemia.
abstract: In the literature, few studies have investigated the effects of melatonin on energy metabolism in skeletal muscle in endotoxemia. We investigated the effects of melatonin on tissue structure, energy metabolism in skeletal muscle, and antioxidant level of rats with endotoxemia. We divided rats into 4 groups, control, lipopolysaccharide (LPS) (20 mg/kg, i.p., single dose), melatonin (10 mg/kg, i.p., three times), and melatonin + LPS. Melatonin was injected i.p. 30 min before and after the 2nd and 4th hours of LPS injection. Antioxidant status was determined by glutathione (GSH) measurement in the blood. Muscle tissue was stained using modified Gomori trichrome (MGT), succinic dehydrogenase (SDH), and cytochrome oxidase (COX) and histological scored. Also the sections were then stained with hematoxylin and eosin. The stained sections were visualized and photographed. Creatine, creatine phosphate, adenosine triphosphate (ATP), adenosine diphosphate (ADP), and adenosine monophosphate (AMP) levels were investigated using high performance liquid chromatography (HPLC) in muscle tissue. In the Melatonin + LPS group, blood GSH levels were increased compared with the LPS group (P<0.01). Melatonin reduced myopathic changes in the LPS group according to the histopathologic findings. In addition, ATP values were increased compared with the LPS group (P<0.05). Our findings showed melatonin treatment prevented muscle damage by increasing ATP and GSH levels in rats with LPS induced endotoxemia.
authors.affiliation: Istanbul University, Institute of Experimental Medicine, Department of Neuroscience, Istanbul, Turkey. eozkok34@hotmail.com.
authors.forename: E
authors.initials: E
authors.lastname: Ozkok
authors.forename: H
authors.initials: H
authors.lastname: Yorulmaz
authors.forename: G
authors.initials: G
authors.lastname: Ates
authors.forename: A
authors.initials: A
authors.lastname: Aksu
authors.forename: N
authors.initials: N
authors.lastname: Balkis
authors.forename: Ö
authors.initials: Ö
authors.lastname: Şahin
authors.forename: S
authors.initials: S
authors.lastname: Tamer
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology / Q000627:therapeutic use*; D019446:Endotoxemia / Q000097:blood / Q000188:drug therapy* / Q000473:pathology; D004734:Energy Metabolism / Q000187:drug effects*; D005978:Glutathione / Q000097:blood; D008070:Lipopolysaccharides; D008297:Male; D008550:Melatonin / Q000494:pharmacology / Q000627:therapeutic use*; D018482:Muscle, Skeletal / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D011897:Random Allocation; D017208:Rats, Wistar
chemical_list: D000975:Antioxidants; D008070:Lipopolysaccharides; D005978:Glutathione; D008550:Melatonin