title: On-Demand Release of Hydrosoluble Drugs from a Paramagnetic Porous Collagen-Based Scaffold.
abstract: The design of a collagen scaffold containing iron oxide nanostructures capped by a TiO2(anatase) layer is reported. The TiO2shell is proposed to perform a dual role: 1) as an innovative and biocompatible cross-linker agent, providing binding sites to the protein moiety, through the well-known TiO2chemical affinity towards carboxyl groups, and 2) as a protective surface layer for the paramagnetic core against oxidation. Simultaneously, the presence of the nanostructures confers to the collagen gel sensitivity to an external stimulus; that is, the application of a magnetic field. The hybrid biomaterial was demonstrated to be nontoxic and is proposed as a smart scaffold for the release of bioactive compounds on demand. The tuneable release of a model protein (myoglobin) upon application of a magnetic field was investigated. Myoglobin was loaded in the microporous material and discharge was induced by consecutive magnet applications, leading to release of the protein with high spatio-temporal and dosage control.
authors.affiliation: Department of Innovation Engineering, University Campus, Ecotekne, Via per Monteroni, 73100, Lecce, Italy.
authors.forename: Simona
authors.identifier: http://orcid.org/0000-0002-5506-5413
authors.initials: S
authors.lastname: Bettini
authors.affiliation: Department of Innovation Engineering, University Campus, Ecotekne, Via per Monteroni, 73100, Lecce, Italy.
authors.forename: Valentina
authors.initials: V
authors.lastname: Bonfrate
authors.affiliation: Department of Innovation Engineering, University Campus, Ecotekne, Via per Monteroni, 73100, Lecce, Italy.
authors.forename: Marta
authors.initials: M
authors.lastname: Madaghiele
authors.affiliation: Department of Innovation Engineering, University Campus, Ecotekne, Via per Monteroni, 73100, Lecce, Italy.
authors.forename: Luca
authors.initials: L
authors.lastname: Salvatore
authors.affiliation: Centre of Excellence for Nanostructured Materials (CENMAT), INSTM, Unit of Trieste, Dipartimento di Scienze Chimiche e Farmaceutiche, Università di Trieste, via L. Giorgieri 1, 34127, Trieste, Italy.
authors.forename: Zois
authors.initials: Z
authors.lastname: Syrgiannis
authors.affiliation: Department of Cultural Heritage, University of Salento, Via Birago 64, 73100, Lecce, Italy.
authors.forename: Gabriele
authors.initials: G
authors.lastname: Giancane
authors.affiliation: Department of Biological and Environmental Sciences and Technologies, DISTEBA, University of Salento, Via per Arnesano, 73100, Lecce, Italy.
authors.forename: Ludovico
authors.identifier: http://orcid.org/0000-0002-6943-8647
authors.initials: L
authors.lastname: Valli
mesh_terms: D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D003094:Collagen / Q000737:chemistry*; D004337:Drug Carriers / Q000737:chemistry; D052203:Ferrosoferric Oxide / Q000737:chemistry; D060526:Magnetic Fields; D051379:Mice; D046529:Microscopy, Electron, Transmission; D009211:Myoglobin / Q000737:chemistry* / Q000378:metabolism; D041681:NIH 3T3 Cells; D049329:Nanostructures / Q000737:chemistry* / Q000633:toxicity; D016062:Porosity; D013059:Spectrum Analysis, Raman; D013818:Thermogravimetry; D014025:Titanium / Q000737:chemistry
chemical_list: D004337:Drug Carriers; D009211:Myoglobin; C009495:titanium dioxide; D003094:Collagen; D014025:Titanium; D052203:Ferrosoferric Oxide
keywords: bioinorganic chemistry; colloids; drug delivery; magnetic properties; nanostructures