title: Cooperative enhancement of deoxyribozyme activity by chemical modification and added cationic copolymer.
abstract: Deoxyribozymes (DNAzymes) having RNA-cleaving activity have widely been explored as tools for therapeutic and diagnostic purposes. Both the chemical cleaving step and the turnover step should be improved for enhancing overall activity of DNAzymes. We have shown that cationic copolymer enhanced DNAzyme activity by increasing turnover efficacy. In this paper, effectｓ of the copolymer on DNAzymes modified with locked nucleic acids (LNA) or 2'-O-methylated (2'-OMe) nucleic acids were studied. The copolymer increased activity of these chemically modified DNAzymes. More than 30-fold enhancement in multiple-turnover catalytic activity was observed with 2'-OMe-modified DNAzyme in the presence of the copolymer. DNAzyme catalytic activity was successfully enhanced by cooperation of the added copolymer and chemical modification of DNAzyme.
authors.affiliation: Department of Life Science and Technology, Tokyo Institute of Technology , Yokohama , Japan.
authors.forename: Ken
authors.initials: K
authors.lastname: Saito
authors.affiliation: Department of Life Science and Technology, Tokyo Institute of Technology , Yokohama , Japan.
authors.forename: Naohiko
authors.initials: N
authors.lastname: Shimada
authors.affiliation: Department of Life Science and Technology, Tokyo Institute of Technology , Yokohama , Japan.
authors.forename: Atushi
authors.initials: A
authors.lastname: Maruyama
keywords: 101 Self-assembly / Self-organized materials; 211 Scaffold/Tissue engineering/Drug delivery; 2’ O-Methyl RNA; 30 Bio-inspired and biomedical materials; Cationic copolymer; Deoxyribozyme; Locked Nucleic Acid; Turnover reaction