title: Prenylated Xanthones from the Roots of Cudrania tricuspidata as Inhibitors of Lipopolysaccharide-Stimulated Nitric Oxide Production.
abstract: Investigation of the CH2Cl2and EtOAc-soluble fractions of the roots of Cudrania tricuspidata afforded 31 compounds. The structures of the isolated compounds were determined on the basis of spectroscopic data interpretation such as 1D and 2D NMR analysis and elucidated as xanthones with prenyl moieties including four simple xanthones (1-4), five prenylated xanthones with one prenyl moiety (5-7, 20, and 21), and 22 prenylated xanthones with two prenyl moieties (8-19 and 22-31). Among them, three diprenylated xanthones named cudracuspixanthones E-G (16, 29, and 31) were first isolated in nature. Xanthones 2, 5, 6, 12, 19, 21, 22, 25, 27, and 28 showed moderate inhibition of lipopolysaccharide-stimulated nitric oxide production in RAW 264.7 cells, with IC50values ranging from 16.1 to 24.8 µM.
authors.affiliation: College of Pharmacy, Chungbuk National University, Cheongju, Korea.
authors.forename: Yang Hee
authors.initials: YH
authors.lastname: Jo
authors.affiliation: College of Pharmacy, Chungbuk National University, Cheongju, Korea.
authors.forename: Seon Beom
authors.initials: SB
authors.lastname: Kim
authors.affiliation: College of Pharmacy, Chungbuk National University, Cheongju, Korea.
authors.forename: Qing
authors.initials: Q
authors.lastname: Liu
authors.affiliation: College of Pharmacy, Chungbuk National University, Cheongju, Korea.
authors.forename: Bang Yeon
authors.initials: BY
authors.lastname: Hwang
authors.affiliation: College of Pharmacy, Chungbuk National University, Cheongju, Korea.
authors.forename: Mi Kyeong
authors.initials: MK
authors.lastname: Lee
mesh_terms: D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D008070:Lipopolysaccharides / Q000037:antagonists & inhibitors* / Q000494:pharmacology; D051379:Mice; D015394:Molecular Structure; D029586:Moraceae / Q000737:chemistry*; D009569:Nitric Oxide / Q000096:biosynthesis*; D018517:Plant Roots / Q000737:chemistry*; D054876:Prenylation; D000067996:RAW 264.7 Cells / Q000378:metabolism; D013329:Structure-Activity Relationship; D044004:Xanthones / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*
chemical_list: D008070:Lipopolysaccharides; D044004:Xanthones; D009569:Nitric Oxide
keywords: Cudracuspixanthones E-G; Cudrania tricuspidata; Nitric oxide inhibitory activity; Prenylated xanthone