title: The Draft Genome Sequence of Actinokineospora bangkokensis 44EHWTReveals the Biosynthetic Pathway of the Antifungal Thailandin Compounds with Unusual Butylmalonyl-CoA Extender Units.
abstract: We report the draft genome sequence ofActinokineospora bangkokensis44EHWT, the producer of the antifungal polyene compounds, thailandins A and B. The sequence contains 7.45 Mb, 74.1% GC content and 35 putative gene clusters for the biosynthesis of secondary metabolites. There are three gene clusters encoding large polyketide synthases of type I. Annotation of the ORF functions and targeted gene disruption enabled us to identify the cluster for thailandin biosynthesis. We propose a plausible biosynthetic pathway for thailandin, where the unusual butylmalonyl-CoA extender unit is incorporated and results in an untypical side chain.
authors.affiliation: Department of Pharmaceutical Biology and Biotechnology, Institute of Pharmaceutical Sciences, Albert-Ludwigs-University of Freiburg, Stefan-Meier-Straße 19, 79104 Freiburg, Germany. anja.greule@pharmazie.uni-freiburg.de.
authors.forename: Anja
authors.initials: A
authors.lastname: Greule
authors.affiliation: Department of Biotechnology, Faculty of Science, Mahidol University, 272 Rama 6 Road, Bangkok 10400, Thailand. aueng_i@hotmail.com.|Mahidol University and Osaka University Collaborative Research Center for Bioscience and Biotechnology, Bangkok 10400, Thailand. aueng_i@hotmail.com.
authors.forename: Bungonsiri
authors.initials: B
authors.lastname: Intra
authors.affiliation: Department of Pharmaceutical Biology and Biotechnology, Institute of Pharmaceutical Sciences, Albert-Ludwigs-University of Freiburg, Stefan-Meier-Straße 19, 79104 Freiburg, Germany. stefan.flemming@gmx.de.
authors.forename: Stephan
authors.initials: S
authors.lastname: Flemming
authors.affiliation: Department of Pharmaceutical Biology and Biotechnology, Institute of Pharmaceutical Sciences, Albert-Ludwigs-University of Freiburg, Stefan-Meier-Straße 19, 79104 Freiburg, Germany. marcel.rommel@yahoo.de.
authors.forename: Marcel G E
authors.initials: MG
authors.lastname: Rommel
authors.affiliation: Department of Biotechnology, Faculty of Science, Mahidol University, 272 Rama 6 Road, Bangkok 10400, Thailand. watanalai.pan@mahidol.ac.th.|Mahidol University and Osaka University Collaborative Research Center for Bioscience and Biotechnology, Bangkok 10400, Thailand. watanalai.pan@mahidol.ac.th.
authors.forename: Watanalai
authors.initials: W
authors.lastname: Panbangred
authors.affiliation: Department of Pharmaceutical Biology and Biotechnology, Institute of Pharmaceutical Sciences, Albert-Ludwigs-University of Freiburg, Stefan-Meier-Straße 19, 79104 Freiburg, Germany. andreas.bechthold@pharmazie.uni-freiburg.de.
authors.forename: Andreas
authors.initials: A
authors.lastname: Bechthold
mesh_terms: D039903:Actinobacteria / Q000235:genetics* / Q000378:metabolism; D000935:Antifungal Agents / Q000378:metabolism; D001482:Base Composition; D001483:Base Sequence; D053898:Biosynthetic Pathways; D002874:Chromosome Mapping; D059646:Genome Size; D016680:Genome, Bacterial; D005810:Multigene Family
chemical_list: D000935:Antifungal Agents
keywords: Actinokineospora bangkokensis; biosynthetic gene cluster; butylmalonyl-CoA; draft genome sequence; genome mining; polyene; thailandin