title: Identification of Alleles of Puroindoline Genes and Their Effect on Wheat (Triticum aestivumL.) Grain Texture.
abstract: Grain hardness is one of the most important quality characteristics of wheat (Triticum aestivumL.). It is a significant property of wheat grains and relates to milling quality and end product quality. Grain hardness is caused by the presence of puroindoline genes (PinaandPinb). A collection of 25 genotypes of wheat with unusual grain colour (blue aleurone, purple and white pericarp, yellow endosperm) was studied by polymerase chain reaction (PCR) for the diversity withinPinaandPinb(alleles:Pina-D1a,Pina-D1b,Pinb-D1a,Pinb- -D1b,Pinb-D1candPinb-D1d). The endosperm structure was determined by a non-destructive method using light transflectance meter and grain hardness by a texture analyser. Genotype Novosibirskaya 67 and isogenic ANK lines revealed hitherto unknown alleles at the locus for the annealing of primers ofPinb-D1. AllelePinb-D1cwas found to be absent from each genotype. The mealy endosperm ranged from 0 to 100% and grain hardness from 15.10 to 26.87 N per sample.
authors.affiliation: Department of Plant Biology, Faculty of AgriSciences, Mendel University in Brno, Zemědělská 1, CZ-61300 Brno, Czech Republic.
authors.forename: Mária
authors.initials: M
authors.lastname: Presinszká
authors.affiliation: Department of Plant Biology, Faculty of AgriSciences, Mendel University in Brno, Zemědělská 1, CZ-61300 Brno, Czech Republic.
authors.forename: Klára
authors.initials: K
authors.lastname: Štiasna
authors.affiliation: Department of Plant Biology, Faculty of AgriSciences, Mendel University in Brno, Zemědělská 1, CZ-61300 Brno, Czech Republic.
authors.forename: Tomáš
authors.initials: T
authors.lastname: Vyhnánek
authors.affiliation: Department of Plant Biology, Faculty of AgriSciences, Mendel University in Brno, Zemědělská 1, CZ-61300 Brno, Czech Republic.
authors.forename: Václav
authors.initials: V
authors.lastname: Trojan
authors.affiliation: Department of Animal Nutrition and Forage Production, Faculty of AgriSciences, Mendel University in Brno, Zemědělská 1, CZ-61300 Brno, Czech Republic.
authors.forename: Eva
authors.initials: E
authors.lastname: Mrkvicová
authors.affiliation: Department of Food Technology, Faculty of AgriSciences, Mendel University in Brno, Zemědělská 1, CZ-61300 Brno, Czech Republic.
authors.forename: Luděk
authors.initials: L
authors.lastname: Hřivna
authors.affiliation: Department of Plant Biology, Faculty of AgriSciences, Mendel University in Brno, Zemědělská 1, CZ-61300 Brno, Czech Republic.
authors.forename: Ladislav
authors.initials: L
authors.lastname: Havel
keywords: grain hardness; mealiness; vitreousness