title: Origanum majoranaEssential Oil Lacks Mutagenic Activity in theSalmonella/Microsome and Micronucleus Assays.
abstract: The present study aimed to investigate thein vitromutagenic activity ofOriganum majoranaessential oil. The most abundant compounds identified by GC-MS wereγ-terpinene (25.73%),α-terpinene (17.35%), terpinen-4-ol (17.24%), and sabinene (10.8%). Mutagenicity was evaluated by theSalmonella/microsome test using the preincubation procedure on TA98, TA97a, TA100, TA102, and TA1535Salmonella typhimuriumstrains, in the absence or in the presence of metabolic activation. Cytotoxicity was detected at concentrations higher than 0.04μL/plate in the absence of S9 mix and higher than 0.08μL/plate in the presence of S9 mix and no gene mutation increase was observed. For thein vitromammalian cell micronucleus test, V79 Chinese hamster lung fibroblasts were used. Cytotoxicity was only observed at concentrations higher than or equal to 0.05μg/mL. Moreover, when tested in noncytotoxic concentrations,O. majoranaessential oil was not able to induce chromosome mutation. The results from this study therefore suggest thatO. majoranaessential oil is not mutagenic at the concentrations tested in theSalmonella/microsome and micronucleus assays.
authors.affiliation: Programa de Pós-Graduação em Ciências Veterinárias, UFRGS, Porto Alegre, RS, Brazil; Laboratório de Produtos Naturais/Fitoquímica-Farmacologia e Toxicologia Veterinária, Departamento de Farmacologia, UFRGS, Porto Alegre, RS, Brazil.
authors.forename: Andrea Dos Santos
authors.identifier: 0000-0001-5085-2875
authors.initials: AD
authors.lastname: Dantas
authors.affiliation: Laboratório de Farmacognosia e Controle de Qualidade de Fitomedicamentos, Faculdade de Farmácia, UFRGS, Porto Alegre, RS, Brazil.
authors.forename: Luiz Carlos
authors.initials: LC
authors.lastname: Klein-Júnior
authors.affiliation: Laboratório de Reparação de DNA em Eucariotos, Departamento de Biofísica/Centro de Biotecnologia, UFRGS, Porto Alegre, RS, Brazil.
authors.forename: Miriana S
authors.identifier: 0000-0003-4287-2942
authors.initials: MS
authors.lastname: Machado
authors.affiliation: Laboratório de Reparação de DNA em Eucariotos, Departamento de Biofísica/Centro de Biotecnologia, UFRGS, Porto Alegre, RS, Brazil.
authors.forename: Temenouga N
authors.initials: TN
authors.lastname: Guecheva
authors.affiliation: Programa de Pós-Graduação em Ciências Veterinárias, UFRGS, Porto Alegre, RS, Brazil; Laboratório de Produtos Naturais/Fitoquímica-Farmacologia e Toxicologia Veterinária, Departamento de Farmacologia, UFRGS, Porto Alegre, RS, Brazil.
authors.forename: Luciana D
authors.initials: LD
authors.lastname: Dos Santos
authors.affiliation: Laboratório de Produtos Naturais/Fitoquímica-Farmacologia e Toxicologia Veterinária, Departamento de Farmacologia, UFRGS, Porto Alegre, RS, Brazil; Programa de Pós-Graduação em Farmacologia e Terapêutica, UFRGS, Porto Alegre, RS, Brazil.
authors.forename: Régis A
authors.initials: RA
authors.lastname: Zanette
authors.affiliation: Laboratório de Produtos Naturais/Fitoquímica-Farmacologia e Toxicologia Veterinária, Departamento de Farmacologia, UFRGS, Porto Alegre, RS, Brazil.
authors.forename: Fernanda B
authors.initials: FB
authors.lastname: de Mello
authors.affiliation: Laboratório de Reparação de DNA em Eucariotos, Departamento de Biofísica/Centro de Biotecnologia, UFRGS, Porto Alegre, RS, Brazil.
authors.forename: João Antonio
authors.identifier: 0000-0002-5298-932X
authors.initials: JA
authors.lastname: Pêgas Henriques
authors.affiliation: Programa de Pós-Graduação em Ciências Veterinárias, UFRGS, Porto Alegre, RS, Brazil; Laboratório de Produtos Naturais/Fitoquímica-Farmacologia e Toxicologia Veterinária, Departamento de Farmacologia, UFRGS, Porto Alegre, RS, Brazil; Programa de Pós-Graduação em Farmacologia e Terapêutica, UFRGS, Porto Alegre, RS, Brazil.
authors.forename: João Roberto Braga
authors.initials: JR
authors.lastname: de Mello
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D006224:Cricetinae; D005347:Fibroblasts / Q000187:drug effects; D015162:Micronucleus Tests; D008861:Microsomes / Q000187:drug effects; D009152:Mutagenicity Tests; D009153:Mutagens; D009822:Oils, Volatile / Q000494:pharmacology*; D031341:Origanum / Q000737:chemistry*; D012486:Salmonella typhimurium / Q000187:drug effects
chemical_list: D009153:Mutagens; D009822:Oils, Volatile