title: Clinical utility of antimicrobial susceptibility measurement plate covering formulated concentrations of various ophthalmic antimicrobial drugs.
abstract: OBJECTIVE
  The purpose of this study was to evaluate the clinical utility of SG17, an ophthalmic antimicrobial susceptibility measurement plate.
  
  
  METHODS
  This was a multicenter, retrospective, observational study.
  
  
  METHODS
  Using clinical isolates from patients with ocular infections, drug susceptibility testing using the Clinical Laboratory and Standards Institute standards was routinely conducted at five facilities. The minimum inhibitory concentrations of the isolated strains were determined using SG17 at the Research Foundation for Microbial Diseases of Osaka University. The records of antimicrobial drugs used and the therapeutic course were evaluated for all cases. The susceptibility results from SG17 and routine methods used at each facility were compared.
  
  
  RESULTS
  A total of 112 bacterial strains were isolated from 92 patients. Of these cases, keratitis was the most common (52.2%), followed by conjunctivitis (21.7%) and others (26.1%). Principal signs and symptoms resolved in all patients, indicating that therapeutic effects had been achieved. With SG17, drug susceptibility was determined in 98.9% of isolates compared with 30.4% of isolates determined using conventional methods. By adapting the Clinical Laboratory and Standards Institute standards to SG17 results, we found that 91.3% of patients were susceptible and 7.6% were resistant. In five patients, drugs with a resistant evaluation were initially administered with no effect, and the patients were then switched to drugs with a susceptible evaluation with final resolution, indicating agreement of clinical results with SG17.
  
  
  CONCLUSIONS
  SG17 can be used to determine drug susceptibility to antimicrobial agents currently used in ophthalmic practice. SG17 is useful for selecting antimicrobial drugs.
authors.affiliation: Department of Ophthalmology, University of Occupational and Environmental Health, Fukuoka.
authors.forename: Norihiko
authors.initials: N
authors.lastname: Tou
authors.affiliation: Miyata Eye Hospital, Miyazaki.
authors.forename: Ryohei
authors.initials: R
authors.lastname: Nejima
authors.affiliation: Division of Ophthalmology and Visual Science, Faculty of Medicine, Tottori University, Tottori.
authors.forename: Yoshifumi
authors.initials: Y
authors.lastname: Ikeda
authors.affiliation: Department of Ophthalmology, Toho University Sakura Medical Center, Chiba.
authors.forename: Yuichi
authors.initials: Y
authors.lastname: Hori
authors.affiliation: Ideta Eye Hospital, Kumamoto, Japan.
authors.forename: Kaoru
authors.initials: K
authors.lastname: Araki-Sasaki
authors.affiliation: Miyata Eye Hospital, Miyazaki.
authors.forename: Kazunori
authors.initials: K
authors.lastname: Miyata
authors.affiliation: Division of Ophthalmology and Visual Science, Faculty of Medicine, Tottori University, Tottori.
authors.forename: Yoshitsugu
authors.initials: Y
authors.lastname: Inoue
authors.affiliation: Department of Ophthalmology, University of Occupational and Environmental Health, Fukuoka.
authors.forename: Akihiko
authors.initials: A
authors.lastname: Tawara
keywords: Clinical Laboratory and Standards Institute; drug susceptibility; minimum inhibitory concentration; ophthalmic antimicrobial agent