title: Comparison between in-house indirect ELISA and Dot-ELISA for the diagnosis ofFasciola giganticain cattle.
abstract: This survey was done to investigate the efficacy of the in-house indirect ELISA (iELISA) and Dot-ELISA methods Prepared from excretion-secretory (ES Ag) and Crude (Cr Ag) antigens ofFasciolafor sero-diagnosis ofFasciola giganticain cattle. The liver specimens of slaughtered cattle were collected and their liver examined macroscopically and microscopically for infestation to fasciolosis. Sera from two groups of cattle, one infected with fasciolosis (n = 60) and the other non-infected with fasciolosis (n = 60), were used in the iELISA and Dot-ELISA test; grouping based on histopathology results. Except specificity, other parameters such as, sensitivity, accuracy, positive and negative predictive values of both Dot-ELISA and iELISA done with ES Ag were better than those of tests performed with Cr Ag. Interestingly, the reliability of two methods was very good similar to one another.
authors.affiliation: Young Researchers and Elite Club, Marand Branch, Islamic Azad University, Marand, Iran.
authors.forename: Jafar
authors.initials: J
authors.lastname: Arjmand Yamchi
authors.affiliation: Young Researchers and Elite club, Urmia Branch, Islamic Azad University, Urmia, Iran.
authors.forename: Nasser
authors.initials: N
authors.lastname: Hajipour
authors.affiliation: Department of Microbiology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran.
authors.forename: Seyyed Meysam
authors.initials: SM
authors.lastname: Abtahi Froushani
authors.affiliation: Institute of Specific Animals, University of Zabol, Zabol, Iran.
authors.forename: Mojtaba
authors.initials: M
authors.lastname: Keighobadi
keywords: Cattle; Comparison; Fasciola gigantica; In-house Dot-ELISA; In-house i-ELISA