title: Involvement of JAK1, JAK2, and JAK3 in Stimulation of Functional Activity of Mesenchymal Progenitor Cells by Fibroblast Growth Factor.
abstract: We studied the involvement of individual JAK kinases in the realization of the growth potential of mesenchymal precursors under the effect of fibroblast growth factor. The important role of JAK2 and JAK3 in determining the initial level of mitotic activity of progenitor cells and participation of JAK1 in this process under conditions of cytokine stimulation of progenitor cells were demonstrated. Specific inhibitors of these kinases reduced the yield of fibroblast CFU and the rate of their division. Moreover, blockade of JAK1, JAK2, and JAK3 under the effect of fibroblast growth factor was accompanied by an increase in the intensity of progenitor cell differentiation.
authors.affiliation: E. D. Goldberg Research Institute of Pharmacology and Regenerative Medicine, Tomsk, Russia. zgn@pharmso.ru.|National Research Tomsk State University, Tomsk, Russia. zgn@pharmso.ru.
authors.forename: G N
authors.initials: GN
authors.lastname: Zyuz'kov
authors.affiliation: E. D. Goldberg Research Institute of Pharmacology and Regenerative Medicine, Tomsk, Russia.
authors.forename: V V
authors.initials: VV
authors.lastname: Zhdanov
authors.affiliation: E. D. Goldberg Research Institute of Pharmacology and Regenerative Medicine, Tomsk, Russia.
authors.forename: E V
authors.initials: EV
authors.lastname: Udut
authors.affiliation: E. D. Goldberg Research Institute of Pharmacology and Regenerative Medicine, Tomsk, Russia.
authors.forename: L A
authors.initials: LA
authors.lastname: Miroshnichenko
authors.affiliation: E. D. Goldberg Research Institute of Pharmacology and Regenerative Medicine, Tomsk, Russia.
authors.forename: E V
authors.initials: EV
authors.lastname: Simanina
authors.affiliation: E. D. Goldberg Research Institute of Pharmacology and Regenerative Medicine, Tomsk, Russia.
authors.forename: T Yu
authors.initials: TY
authors.lastname: Polyakova
authors.affiliation: E. D. Goldberg Research Institute of Pharmacology and Regenerative Medicine, Tomsk, Russia.
authors.forename: L A
authors.initials: LA
authors.lastname: Stavrova
authors.affiliation: E. D. Goldberg Research Institute of Pharmacology and Regenerative Medicine, Tomsk, Russia.|National Research Tomsk State University, Tomsk, Russia.
authors.forename: V V
authors.initials: VV
authors.lastname: Udut
authors.affiliation: E. D. Goldberg Research Institute of Pharmacology and Regenerative Medicine, Tomsk, Russia.
authors.forename: M Yu
authors.initials: MY
authors.lastname: Minakova
authors.affiliation: E. D. Goldberg Research Institute of Pharmacology and Regenerative Medicine, Tomsk, Russia.
authors.forename: A M
authors.initials: AM
authors.lastname: Dygai
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology / Q000187:drug effects / Q000201:enzymology; D002454:Cell Differentiation / Q000187:drug effects; D003114:Colony-Forming Units Assay; D005346:Fibroblast Growth Factors / Q000494:pharmacology*; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects / Q000201:enzymology; D005786:Gene Expression Regulation; D053613:Janus Kinase 1 / Q000037:antagonists & inhibitors / Q000235:genetics* / Q000378:metabolism; D053614:Janus Kinase 2 / Q000037:antagonists & inhibitors / Q000235:genetics* / Q000378:metabolism; D053616:Janus Kinase 3 / Q000037:antagonists & inhibitors / Q000235:genetics* / Q000378:metabolism; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000187:drug effects* / Q000201:enzymology; D051379:Mice; D008808:Mice, Inbred CBA; D010880:Piperidines / Q000494:pharmacology; D061251:Primary Cell Culture; D047428:Protein Kinase Inhibitors / Q000494:pharmacology; D011743:Pyrimidines / Q000494:pharmacology; D011758:Pyrroles / Q000494:pharmacology; D015398:Signal Transduction; D020032:Tyrphostins / Q000494:pharmacology
chemical_list: D010880:Piperidines; D047428:Protein Kinase Inhibitors; D011743:Pyrimidines; D011758:Pyrroles; D020032:Tyrphostins; C095512:alpha-cyano-(3,4-dihydroxy)-N-benzylcinnamide; D005346:Fibroblast Growth Factors; C479163:tofacitinib; C507905:Jak1 protein, mouse; C507926:Jak2 protein, mouse; C507935:Jak3 protein, mouse; D053613:Janus Kinase 1; D053614:Janus Kinase 2; D053616:Janus Kinase 3
keywords: JAK; fibroblast growth factor; progenitor cells; regenerative medicine; signal pathways