title: Effect of olive leaf,Satureja khuzestanica, andAllium sativumextracts onGiardia lambliacysts compared with metronidazole in vitro.
abstract: Giardia lambliais one of the common causes of worldwide diarrhea in children. Appropriate medicinal treatment for giardiasis is available but there are some evidences of drug resistance, insufficient efficacy, and unpleasant side effects. In order to reach a more natural drug with suitable efficacy and the lowest side effects, the effects of the hydroalcoholic extracts of olive leaf,Satureja khuzestanica, andAllium sativumonG. lambliacysts were evaluated in vitro, as well as antigiardial effect of the extracts was compared with metronidazole as the drug of choice. 2 and 5 mg of the plants extracts and powder of metronidazole 250 mg pills were added to 1 ml ofG. lambliacysts suspension (containing 5,000 cyst/ml normal saline), and the percentages of bioavailability ofG. lambliacysts were examined at the 2nd and 4th h after exposure and in 4 and 37 °C temperatures using eosin 0.1 % and a haemocytometer. The data were analyzed by multiway ANOVA test, Tukey's test, and the SPSS software, version 18. The examinations demonstrated that olive leaf extract had the most fatality rate onG. lambliacysts in vitro (37.90 ± 7.01 %), followed by the extract ofS. khuzestanica(32.52 ± 9.07 %). Metronidazole 250 mg pills had relatively effective fatality rate onG. lambliacysts in vitro (28.75 ± 10.30 %), whereasA. sativum(garlic) had the lowest fatality effect onG. lambliacysts in vitro (22.65 ± 10.47 %). With respect to higher fatality effect of olive leaf andS. khuzestanicaextracts compared with metronidazole in vitro, these plants can be used as suitable candidates to make new antigiardial drugs with low side effects and without drug resistance in the treatment of giardiasis in children.
authors.affiliation: Department of Medical Parasitology and Mycology, Faculty of Medicine, Lorestan University of Medical Sciences, Khorramabad, Iran.
authors.forename: Sh
authors.initials: S
authors.lastname: Fallahi
authors.affiliation: Department of Parasitology and Mycology, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
authors.forename: A
authors.initials: A
authors.lastname: Rostami
authors.affiliation: Department of Pharmacology, Faculty of Medicine, Lorestan University of Medical Sciences, Khorramabad, Iran.
authors.forename: B
authors.initials: B
authors.lastname: Delfan
authors.affiliation: Faculty of Medicine, Lorestan University of Medical Sciences, Khorramabad, Iran.
authors.forename: Y
authors.initials: Y
authors.lastname: Pournia
authors.affiliation: Young Researcher Club, Khorramabad Branch, Islamic Azad University, Khorramabad, Iran.
authors.forename: M
authors.initials: M
authors.lastname: Rashidipour
keywords: Allium sativum; Giardia lamblia; In vitro; Metronidazole; Olive leaf; Satureja khuzestanica