title: Racemic alkaloids from the fungus Ganoderma cochlear.
abstract: Seven pairs of new alkaloid enantiomers, ganocochlearines C-I (1, 3-8), and three pairs of known alkaloids were isolated from the fruiting bodies of Ganoderma cochlear. The chemical structures of new compounds were elucidated on the basis of 1D and 2D NMR data. The absolute configurations of compounds 1, 3-10 were assigned by ECD calculations. Biological activities of these isolates against renal fibrosis were accessed in rat normal or diseased renal interstitial fibroblast cells. Importantly, the plausible biosynthetic pathway for this class of alkaloids was originally proposed.
authors.affiliation: Henan University of Traditional Chinese Medicine, Zhengzhou, 450008, PR China; State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, 650201, PR China.
authors.forename: Xin-Long
authors.initials: XL
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, 650201, PR China; Faculty of Life Science and Engineering, Kunming University of Science and Technology, Kunming, 650500, PR China.
authors.forename: Man
authors.initials: M
authors.lastname: Dou
authors.affiliation: State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, 650201, PR China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Luo
authors.affiliation: State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, 650201, PR China; Guangdong Pharmaceutical University, Guangzhou, 510006, PR China.
authors.forename: Li-Zhi
authors.initials: LZ
authors.lastname: Cheng
authors.affiliation: State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, 650201, PR China.
authors.forename: Yong-Ming
authors.initials: YM
authors.lastname: Yan
authors.affiliation: Faculty of Life Science and Engineering, Kunming University of Science and Technology, Kunming, 650500, PR China.
authors.forename: Rong-Tao
authors.initials: RT
authors.lastname: Li
authors.affiliation: Henan University of Traditional Chinese Medicine, Zhengzhou, 450008, PR China; State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, 650201, PR China. Electronic address: yxcheng@mail.kib.ac.cn.
authors.forename: Yong-Xian
authors.initials: YX
authors.lastname: Cheng
mesh_terms: D000470:Alkaloids / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D000818:Animals; D002460:Cell Line; D005347:Fibroblasts / Q000187:drug effects*; D005355:Fibrosis; D048690:Fruiting Bodies, Fungal / Q000737:chemistry; D032902:Ganoderma / Q000737:chemistry*; D007668:Kidney / Q000166:cytology / Q000473:pathology*; D015394:Molecular Structure; D051381:Rats; D013237:Stereoisomerism
chemical_list: D000470:Alkaloids
keywords: Alkaloids; Biosynthesis; ECD calculations; Ganoderma cochlear; Racemates