title: Genotypic distribution of HHV-8 in AIDS individuals without and with Kaposi sarcoma: Is genotype B associated with better prognosis of AIDS-KS?
abstract: AIDS-associated Kaposi's sarcoma (AIDS-KS) caused by human herpes virus 8 (HHV-8) is the most severe and resistant form of KS tumor. Our aim was to verify whether there is an association between HHV-8 variability and development of AIDS-KS in Brazil by comparing the HHV-8 variability between individuals without and with KS. Saliva samples and blood, when available, were analyzed by polymerase chain reaction (PCR) techniques for detection of the fragments of ORF K1 of HHV-8, which were then genotyped and analyzed regarding the genetic variability. Our study described 106 positive cases for HHV-8 in the saliva from 751 AIDS patients without previous KS. In addition, we performed a phylogenetic analysis of HHV-8 in 34 of the 106 AIDS patients without KS and in 33 of the 37 patients with active KS. The distribution of HHV-8 genotypes A, B, C, and F in AIDS individuals was indistinguishable by comparing non-KS and KS groups, as well as regarding ethnicity. Considering the KS group, genotype B was associated with better prognosis of KS tumor. Interestingly, we found a particular profile of diversity within clade C and 2 recombinant patterns of HHV-8 in the saliva of AIDS individuals without KS. We emphasize the need to achieve standard genotyping protocol for ORF K1 amplification, thus allowing for substantial detection of HHV-8 variants. Our findings can shed light on the role of HHV-8 variability in the pathogenesis of AIDS-KS.
authors.affiliation: Institute of Tropical Medicine Laboratory of Virology LIM52 Department of Infectious Diseases, Clinics Hospital of the School of Medicine Institute of Tropical Medicine Laboratory of Seroepidemiology, University of São Paulo, São Paulo, Brazil Institute of Virology, University of Bonn Medical Centre, Bonn, German Centre for Infection Research (DZIF), partner site Bonn-Cologne, Germany Pathology Department of the School of Dentistry, University of São Paulo, São Paulo, Brazil.
authors.forename: Tania Regina
authors.initials: TR
authors.lastname: Tozetto-Mendoza
authors.forename: Karim Yaqub
authors.initials: KY
authors.lastname: Ibrahim
authors.forename: Adriana Fumie
authors.initials: AF
authors.lastname: Tateno
authors.forename: Cristina Mendes
authors.initials: CM
authors.lastname: de Oliveira
authors.forename: Laura Massami
authors.initials: LM
authors.lastname: Sumita
authors.forename: Maria Carmem Arroyo
authors.initials: MCA
authors.lastname: Sanchez
authors.forename: Expedito José
authors.initials: EJ
authors.lastname: Luna
authors.forename: Ligia Camara
authors.initials: LC
authors.lastname: Pierrotti
authors.forename: Jan Felix
authors.initials: JF
authors.lastname: Drexler
authors.forename: Paulo Henrique
authors.initials: PH
authors.lastname: Braz-Silva
authors.forename: Claudio Sérgio
authors.initials: CS
authors.lastname: Pannuti
authors.forename: Camila Malta
authors.initials: CM
authors.lastname: Romano
mesh_terms: D017088:AIDS-Related Opportunistic Infections / Q000235:genetics* / Q000821:virology*; D000163:Acquired Immunodeficiency Syndrome / Q000235:genetics*; D001938:Brazil; D003430:Cross-Sectional Studies; D004279:DNA, Viral / Q000032:analysis; D005260:Female; D005814:Genes, Viral; D014644:Genetic Variation; D005838:Genotype; D006801:Humans; D008297:Male; D016366:Open Reading Frames; D010802:Phylogeny; D011379:Prognosis; D060888:Real-Time Polymerase Chain Reaction; D012463:Saliva / Q000821:virology; D012514:Sarcoma, Kaposi / Q000235:genetics* / Q000821:virology*; D014764:Viral Proteins / Q000235:genetics*
chemical_list: D004279:DNA, Viral; C111600:K1 protein, Human herpesvirus 8; D014764:Viral Proteins