title: High-throughput ultra high performance liquid chromatography coupled to quadrupole time-of-flight mass spectrometry method for the rapid analysis and characterization of multiple constituents of Radix Polygalae.
abstract: Radix Polygalae, the dried roots of Polygala tenuifolia and P. sibirica, is one of the most well-known traditional Chinese medicinal plants. It is an important medicinal plant that has been used as a sedative and to improve memory for a number of years in most of Asia. However, the in vivo constituents of the multiple constituents from Radix Polygalae remain unknown. In the current study, ultra high performance liquid chromatography coupled to quadrupole time-of-flight mass spectrometry and the MarkerLynxTMsoftware combined with multiple data processing approach were used to study the constituents in vitro and in vivo. A rapid and efficient method for the characterization of multiple constituents in the herbal medicine Radix Polygalae by ultra high performance liquid chromatography coupled to quadrupole time-of-flight mass spectrometry is described. In total, 35 compounds in the Radix Polygalae and 13 compounds absorbed into blood were characterized. Of the 35 compounds in vitro, ten were reported for first time. In the 13 compounds in vivo, six were prototype components and seven were metabolites were also elucidated for first time. This work narrowed the range of screening the potentially bioactive components and provided a basis for the quality control and mechanism of action.
authors.affiliation: Sino-America Chinmedomics Technology Cooperation Center, National TCM Key Laboratory of Serum Pharmacochemistry, Research Center of Chinmedomics (State Administration of TCM), Department of Pharmaceutical Analysis, Laboratory of Metabolomics, Heilongjiang University of Chinese Medicine, Harbin, P.R. China.
authors.forename: Chang
authors.initials: C
authors.lastname: Liu
authors.affiliation: Sino-America Chinmedomics Technology Cooperation Center, National TCM Key Laboratory of Serum Pharmacochemistry, Research Center of Chinmedomics (State Administration of TCM), Department of Pharmaceutical Analysis, Laboratory of Metabolomics, Heilongjiang University of Chinese Medicine, Harbin, P.R. China.
authors.forename: Aihua
authors.initials: A
authors.lastname: Zhang
authors.affiliation: Sino-America Chinmedomics Technology Cooperation Center, National TCM Key Laboratory of Serum Pharmacochemistry, Research Center of Chinmedomics (State Administration of TCM), Department of Pharmaceutical Analysis, Laboratory of Metabolomics, Heilongjiang University of Chinese Medicine, Harbin, P.R. China.
authors.forename: Guang-Li
authors.initials: GL
authors.lastname: Yan
authors.affiliation: Sino-America Chinmedomics Technology Cooperation Center, National TCM Key Laboratory of Serum Pharmacochemistry, Research Center of Chinmedomics (State Administration of TCM), Department of Pharmaceutical Analysis, Laboratory of Metabolomics, Heilongjiang University of Chinese Medicine, Harbin, P.R. China.
authors.forename: Hui
authors.initials: H
authors.lastname: Shi
authors.affiliation: Sino-America Chinmedomics Technology Cooperation Center, National TCM Key Laboratory of Serum Pharmacochemistry, Research Center of Chinmedomics (State Administration of TCM), Department of Pharmaceutical Analysis, Laboratory of Metabolomics, Heilongjiang University of Chinese Medicine, Harbin, P.R. China.
authors.forename: Hui
authors.initials: H
authors.lastname: Sun
authors.affiliation: Sino-America Chinmedomics Technology Cooperation Center, National TCM Key Laboratory of Serum Pharmacochemistry, Research Center of Chinmedomics (State Administration of TCM), Department of Pharmaceutical Analysis, Laboratory of Metabolomics, Heilongjiang University of Chinese Medicine, Harbin, P.R. China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Han
authors.affiliation: Sino-America Chinmedomics Technology Cooperation Center, National TCM Key Laboratory of Serum Pharmacochemistry, Research Center of Chinmedomics (State Administration of TCM), Department of Pharmaceutical Analysis, Laboratory of Metabolomics, Heilongjiang University of Chinese Medicine, Harbin, P.R. China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: Sino-America Chinmedomics Technology Cooperation Center, National TCM Key Laboratory of Serum Pharmacochemistry, Research Center of Chinmedomics (State Administration of TCM), Department of Pharmaceutical Analysis, Laboratory of Metabolomics, Heilongjiang University of Chinese Medicine, Harbin, P.R. China.
authors.forename: Xijun
authors.initials: X
authors.lastname: Wang
mesh_terms: D002623:Chemistry Techniques, Analytical / Q000379:methods*; D002851:Chromatography, High Pressure Liquid; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D013058:Mass Spectrometry; D018517:Plant Roots / Q000737:chemistry; D031801:Polygala / Q000737:chemistry*; D053719:Tandem Mass Spectrometry
chemical_list: D004365:Drugs, Chinese Herbal
keywords: Radix Polygalae; multiple constituents; multiple data processing approach; traditional Chinese medicine