title: Clickable Polymeric Coating for Glycan Microarrays.
abstract: The interaction of carbohydrates with a variety of biological targets, including antibodies, proteins, viruses, and cells are of utmost importance in many aspects of biology. Glycan microarrays are increasingly used to determine the binding specificity of glycan-binding proteins. In this study, a novel microarray support is reported for the fabrication of glycan arrays that combines the higher sensitivity of a layered Si-SiO2surface with a novel polymeric coating easily modifiable by subsequent click reaction. The alkyne-containing copolymer, adsorbed from an aqueous solution, produces a coating by a single step procedure and serves as a soft, tridimensional support for the oriented immobilization of carbohydrates via azide/alkyne Cu (I) catalyzed "click" reaction. The advantages of a functional 3D polymer coating making use of a click chemistry immobilization are combined with the high fluorescence sensitivity and superior signal-to-noise ratio of a Si-SiO2substrate. The proposed approach enables the attachment of complex sugars on a silicon oxide surface by a method that does not require skilled personnel and chemistry laboratories.
authors.affiliation: Istituto di Chimica del Riconoscimento Molecolare, CNR, Via Mario Bianco 9, 20131, Milan, Italy.
authors.forename: Caterina
authors.initials: C
authors.lastname: Zilio
authors.affiliation: Istituto di Chimica del Riconoscimento Molecolare, CNR, Via Mario Bianco 9, 20131, Milan, Italy. laura.sola@icrm.cnr.it.
authors.forename: Laura
authors.initials: L
authors.lastname: Sola
authors.affiliation: Istituto di Chimica del Riconoscimento Molecolare, CNR, Via Mario Bianco 9, 20131, Milan, Italy.
authors.forename: Marina
authors.initials: M
authors.lastname: Cretich
authors.affiliation: Dipartimento di Chimica, Università degli Studi di Milano, Via Golgi 19, 20133, Milan, Italy.
authors.forename: Anna
authors.initials: A
authors.lastname: Bernardi
authors.affiliation: Istituto di Chimica del Riconoscimento Molecolare, CNR, Via Mario Bianco 9, 20131, Milan, Italy.
authors.forename: Marcella
authors.initials: M
authors.lastname: Chiari
mesh_terms: D057930:Click Chemistry / Q000379:methods*; D005453:Fluorescence; D046228:Microarray Analysis / Q000379:methods*; D011108:Polymers / Q000138:chemical synthesis / Q000737:chemistry*; D011134:Polysaccharides / Q000737:chemistry*; D012825:Silicon / Q000737:chemistry
chemical_list: D011108:Polymers; D011134:Polysaccharides; D012825:Silicon
keywords: Click chemistry; Glycan microarray; Oriented immobilization; Polymeric coating