title: Production of Recombinant Polypeptides Containing One GA-Module and Analysis of Their Ability to Bind to Human Albumin.
abstract: Surface proteins of many bacterial species interact with human serum albumin (HSA) via a special region of amino acid sequence termed GA module. For instance, surface peptostreptococcal albumin-binding protein of anaerobic bacteria Peptostreptococcus magnus contains one HSA-binding GA-module. Protein G from group G and C Streptococcus strains isolated from humans has HSA-binding region consisting of three GA-modules. HSA-binding protein containing two GA-modules was found in strains of group G Streptococcus of animal origin. We obtained two recombinant polypeptides GA1 and GA2 congaing one GA-module each. Recombinant polypeptide with two GA-modules binds HSA with a much higher affinity than polypeptides GA1 and GA2 containing one GA-module. Polypeptide with the second GAmodule more effectively binds HSA than polypeptides with the GA-module.
authors.affiliation: Department of Molecular Microbiology Institute of Experimental Medicine, St. Petersburg, Russia.
authors.forename: E A
authors.initials: EA
authors.lastname: Bormotova
authors.affiliation: Department of Molecular Microbiology Institute of Experimental Medicine, St. Petersburg, Russia. tvgupalova@rambler.ru.
authors.forename: T V
authors.initials: TV
authors.lastname: Gupalova
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000096:biosynthesis / Q000737:chemistry* / Q000235:genetics; D001483:Base Sequence; D001665:Binding Sites; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000737:chemistry / Q000235:genetics; D015870:Gene Expression; D006801:Humans; D011485:Protein Binding; D054730:Protein Interaction Domains and Motifs; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics; D012709:Serum Albumin / Q000737:chemistry*; D012996:Solutions; D013291:Streptococcus / Q000737:chemistry* / Q000235:genetics
chemical_list: D001426:Bacterial Proteins; D011994:Recombinant Proteins; D012709:Serum Albumin; D012996:Solutions
keywords: GA-module; Streptococcus group G; human serum albumin binding polypeptide