title: Candida guilliermondii as a potential biocatalyst for the production of long-chain α,ω-dicarboxylic acids.
abstract: OBJECTIVE
  To explore Candida guilliermondii for the production of long-chain dicarboxylic acids (DCA), we performed metabolic pathway engineering aiming to prevent DCA consumption during β-oxidation, but also to increase its production via the ω-oxidation pathway.
  
  
  RESULTS
  We identified the major β- and ω-oxidation pathway genes in C. guilliermondii and performed first steps in the strain improvement. A double pox disruption mutant was created that slowed growth with oleic acid but showed accelerated DCA degradation. Increase in DCA production was achieved by homologous overexpression of a plasmid borne cytochrome P450 monooxygenase gene.
  
  
  CONCLUSIONS
  C. guilliermondii is a promising biocatalyst for DCA production but further insight into its fatty acid metabolism is necessary.
authors.affiliation: Fraunhofer Institute for Interfacial Engineering and Biotechnology IGB, Stuttgart, Germany.
authors.forename: Nicole
authors.identifier: http://orcid.org/0000-0002-1096-0043
authors.initials: N
authors.lastname: Werner
authors.affiliation: Fraunhofer Institute for Interfacial Engineering and Biotechnology IGB, Stuttgart, Germany.
authors.forename: Miriam
authors.initials: M
authors.lastname: Dreyer
authors.affiliation: Fraunhofer Institute for Interfacial Engineering and Biotechnology IGB, Stuttgart, Germany.
authors.forename: Wenke
authors.initials: W
authors.lastname: Wagner
authors.affiliation: Groupe d'Etude des Interactions Hôte-Pathogène, EA 3142, Université d'Angers, Angers, France.
authors.forename: Nicolas
authors.initials: N
authors.lastname: Papon
authors.affiliation: Fraunhofer Institute for Interfacial Engineering and Biotechnology IGB, Stuttgart, Germany.
authors.forename: Steffen
authors.initials: S
authors.lastname: Rupp
authors.affiliation: Fraunhofer Institute for Interfacial Engineering and Biotechnology IGB, Stuttgart, Germany. susanne.zibek@igb.fraunhofer.de.
authors.forename: Susanne
authors.initials: S
authors.lastname: Zibek
mesh_terms: D055162:Biocatalysis; D002175:Candida / Q000235:genetics / Q000378:metabolism*; D002855:Chromatography, Thin Layer; D003998:Dicarboxylic Acids / Q000378:metabolism*; D015966:Gene Expression Regulation, Fungal; D005800:Genes, Fungal; D009154:Mutation / Q000235:genetics; D010084:Oxidation-Reduction; D010802:Phylogeny
chemical_list: D003998:Dicarboxylic Acids
keywords: Acyl-CoA oxidase; Cytochrome P450 hydroxylase; Genetic engineering; Long chain dicarboxylic acids; Oleic acid; β-Oxidation; ω-Oxidation