title: Assay of hemoglobin A1cusing lectin from Aleuria aurantia.
abstract: Hemoglobin A1c(HbA1c) has an N-terminal fructosyl valine on the β-chain, and this modification is caused by the non-enzymatic glycosylation of hemoglobin (Hb). The relative concentration ratio of HbA1cto total Hb is an important biomarker for the diagnosis of diabetes. HbA1c-binding lectins were screened from 29 sources of lectin, and the lectin from Aleuria aurantia (AAL) was revealed to have higher affinity to HbA1cthan to Hb. The concentration of HbA1cwas determined by lectin-based enzyme-linked immunosorbent assay (ELISA) using the AAL lectin. Higher reproducibility of the assay was observed at 4 °C than at 25 and 37 °C. This observation is consistent with the known temperature-dependent behavior of lectins. Preincubation of HbA1cwith an anti-HbA1cantibody inhibited the binding, suggesting that AAL binds to the N-terminal fructosyl valine epitope of HbA1c. Higher inhibitory effect was observed for 10 mM D-fructose than for the same concentrations of L-fucose, D-fucose, or D-glucose.
authors.affiliation: Department of Applied Molecular Chemistry, Graduate School of Industrial Technology, Nihon University, 1-2-1, Izumichou, Narashino, Chiba, 275-8575, Japan.
authors.forename: Masato
authors.initials: M
authors.lastname: Kabata
authors.affiliation: Department of Applied Molecular Chemistry, College of Industrial Technology, Nihon University, 1-2-1, Izumichou, Narashino, Chiba, 275-8575, Japan.
authors.forename: Erina
authors.initials: E
authors.lastname: Hase
authors.affiliation: Department of Applied Molecular Chemistry, College of Industrial Technology, Nihon University, 1-2-1, Izumichou, Narashino, Chiba, 275-8575, Japan.|Nacalai tesque Inc., Kyoto, Japan.
authors.forename: Kouta
authors.initials: K
authors.lastname: Kimura
authors.affiliation: Product Development Laboratory, J-oil Mills Inc., 11, Kagetoricho Totsuka-ku, Yokohama, Kanagawa, 245-0064, Japan.
authors.forename: Yuka
authors.initials: Y
authors.lastname: Kobayashi
authors.affiliation: Product Development Laboratory, J-oil Mills Inc., 11, Kagetoricho Totsuka-ku, Yokohama, Kanagawa, 245-0064, Japan.
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Ueno
authors.affiliation: Department of Applied Molecular Chemistry, Graduate School of Industrial Technology, Nihon University, 1-2-1, Izumichou, Narashino, Chiba, 275-8575, Japan. yoshimune.kazuaki@nihon-u.ac.jp.|Department of Applied Molecular Chemistry, College of Industrial Technology, Nihon University, 1-2-1, Izumichou, Narashino, Chiba, 275-8575, Japan. yoshimune.kazuaki@nihon-u.ac.jp.
authors.forename: Kazuaki
authors.initials: K
authors.lastname: Yoshimune
keywords: Aleuria aurantia; Hemoglobin A1c; Lectin; Lectin-based enzyme-linked immunosorbent assay