title: Rapid synthesis of silver nanoparticles byPseudomonas stutzeriisolated from textile soil under optimised conditions and evaluation of their antimicrobial and cytotoxicity properties.
abstract: Present study utilised textile soil isolated bacteriumPseudomonas stutzerito synthesise extracellular silver nanoparticles (AgNPs) under optimised conditions. The synthesised AgNPs were characterised using ultraviolet-visible spectroscopy, Fourier transform infrared spectroscopy (FTIR) and transmission electron microscopy (TEM). Optimisation showed AgNPs synthesis within 8 h using 2mM Ag nitrate at pH9, temperature 80°C and maximum absorbance toward 400 nm. TEM analysis revealed spherical shape AgNPs and reduction in size upto 8 nm was observed under optimised conditions. FTIR spectra confirmed presence of proteins bound to AgNPs act as reducing agent. AgNPs showed strong antibacterial activity against multi-drug resistant (MDR)Escherichia coliandKlebsiella pneumoniaeas demonstrated by disc diffusion and colony forming unit assays. Zone of inhibition increased with increasing concentration of AgNPs with maximum of 19 mm againstE. coliand 17 mm againstK. pneumoniaeat concentration of 2 μg/disc. Furthermore, AgNPs did not show any cytotoxic effects on human epithelial cells as demonstrated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay even at 2 μg/ml concentration of AgNPs. The results of the present study suggest that AgNPs can be synthesised rapidly under optimised conditions and show strong antimicrobial property against MDR pathogens without having toxicity effect on human epithelial cells.
authors.affiliation: AMITY Institute of Biotechnology, AMITY University Rajasthan, Jaipur 302006, India.
authors.forename: Nishant
authors.initials: N
authors.lastname: Rajora
authors.affiliation: AMITY Institute of Biotechnology, AMITY University Rajasthan, Jaipur 302006, India.
authors.forename: Sanket
authors.initials: S
authors.lastname: Kaushik
authors.affiliation: AMITY Institute of Biotechnology, AMITY University Rajasthan, Jaipur 302006, India. ajyoti@jpr.amity.edu.
authors.forename: Anupam
authors.initials: A
authors.lastname: Jyoti
authors.affiliation: AMITY Institute of Biotechnology, AMITY University Rajasthan, Jaipur 302006, India.
authors.forename: Shanker L
authors.initials: SL
authors.lastname: Kothari
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002478:Cells, Cultured; D024901:Drug Resistance, Multiple, Bacterial; D004847:Epithelial Cells / Q000187:drug effects; D004926:Escherichia coli / Q000187:drug effects; D006801:Humans; D007711:Klebsiella pneumoniae / Q000187:drug effects; D053768:Metal Nanoparticles; D008826:Microbial Sensitivity Tests; D046529:Microscopy, Electron, Transmission; D044223:Pseudomonas stutzeri / Q000378:metabolism*; D012834:Silver; D012987:Soil; D017550:Spectroscopy, Fourier Transform Infrared; D013784:Textiles
chemical_list: D000900:Anti-Bacterial Agents; D012987:Soil; D012834:Silver