title: Manipulation of cell membrane using carbon nanotube scaffold as a photoresponsive stimuli generator.
abstract: We describe, for the first time, the perforation of the cell membrane in the targeted single cell based on the nanosecond pulsed near-infrared (NIR) laser irradiation of a thin film of carbon nanotubes that act as an effective photon absorber as well as stimuli generator. When the power of NIR laser is over 17.5μJ/pulse, the cell membrane after irradiation is irreversibly disrupted and results in cell death. In sharp contrast, the perforation of the cell membrane occurs at suitable laser power (∼15μJ/pulse) without involving cell termination.
authors.affiliation: Department of Applied Chemistry, Graduate School of Engineering, Kyushu University, 744 Motooka Nishi-ku, Fukuoka 819-0395, Japan.
authors.forename: Takao
authors.initials: T
authors.lastname: Sada
authors.affiliation: Department of Applied Chemistry, Graduate School of Engineering, Kyushu University, 744 Motooka Nishi-ku, Fukuoka 819-0395, Japan; International Institute for Carbon-Neutral Energy Research (WPI-I2CNER), Kyushu University, Japan.
authors.forename: Tsuyohiko
authors.initials: T
authors.lastname: Fujigaya
authors.affiliation: Department of Applied Chemistry, Graduate School of Engineering, Kyushu University, 744 Motooka Nishi-ku, Fukuoka 819-0395, Japan; International Institute for Carbon-Neutral Energy Research (WPI-I2CNER), Kyushu University, Japan; Core Research for Evolutionary Science and Technology (JST-CREST), 5 Sanbancho, Chiyoda-ku, Tokyo, 102-0075, Japan.
authors.forename: Naotoshi
authors.initials: N
authors.lastname: Nakashima
keywords: carbon nanotubes; cell perforation; cell scaffold; nanosecond pulse laser; near-IR laser