title: Saliva exosomes from pancreatic tumor-bearing mice modulate NK cell phenotype and antitumor cytotoxicity.
abstract: Tumor exosomes are emerging as antitumor immunity regulators; however, their effects on secondary exosome secretion by distal organs have not been explored. We have previously demonstrated that suppression of exosomes at the distal tumor site of pancreatic ductal adenocarcinoma (PDAC) ablated the development of salivary biomarker profile. Here, we explore the function of salivary exosomes from tumor-bearing mice in immune surveillance. We provide evidence that salivary exosomes from mice with PDAC exhibit a suppressive effect that results in reduced tumor-killing capacity by NK cells. Salivary exosomes from mice with PDAC where pancreatic tumors were engineered to suppress exosome biogenesis failed to suppress NK cell cytotoxic potential against tumor cells, as opposed to salivary exosomes from mice with PDAC with normal tumor exosome biogenesis. These results reveal an important and previously unknown mechanism of antitumor immune regulation and provide new insights into our understanding of the alterations of this biofluid during tumor development.-Katsiougiannis, S., Chia, D., Kim, Y., Singh, R. P., Wong, D. T. W. Saliva exosomes from pancreatic tumor-bearing mice modulate NK cell phenotype and antitumor cytotoxicity.
authors.affiliation: Center for Oral/Head and Neck Oncology Research, School of Dentistry, University of California Los Angeles, Los Angeles, California, USA.
authors.forename: Stergios
authors.initials: S
authors.lastname: Katsiougiannis
authors.affiliation: Jonsson Comprehensive Cancer Center, University of California Los Angeles, Los Angeles, California, USA.|Department of Pathology and Laboratory Medicine, David Geffen School of Medicine, University of California Los Angeles, Los Angeles, California, USA.
authors.forename: David
authors.initials: D
authors.lastname: Chia
authors.affiliation: Center for Oral/Head and Neck Oncology Research, School of Dentistry, University of California Los Angeles, Los Angeles, California, USA.|Jonsson Comprehensive Cancer Center, University of California Los Angeles, Los Angeles, California, USA.|Laboratory of Stem Cell and Cancer Epigenetic Research, University of California Los Angeles, Los Angeles, California, USA.
authors.forename: Yong
authors.initials: Y
authors.lastname: Kim
authors.affiliation: Division of Rheumatology, David Geffen School of Medicine, University of California Los Angeles, Los Angeles, California, USA; and.|Veterans Affairs Greater Los Angeles Healthcare System, Los Angeles, California, USA.
authors.forename: Ram P
authors.initials: RP
authors.lastname: Singh
authors.affiliation: Center for Oral/Head and Neck Oncology Research, School of Dentistry, University of California Los Angeles, Los Angeles, California, USA; dwong@dentistry.ucla.edu.|Jonsson Comprehensive Cancer Center, University of California Los Angeles, Los Angeles, California, USA.
authors.forename: David T W
authors.initials: DT
authors.lastname: Wong
mesh_terms: D000818:Animals; D021441:Carcinoma, Pancreatic Ductal / Q000276:immunology*; D045744:Cell Line, Tumor; D003602:Cytotoxicity, Immunologic; D055354:Exosomes / Q000276:immunology*; D005260:Female; D007155:Immunologic Factors / Q000378:metabolism*; D007694:Killer Cells, Natural / Q000276:immunology*; D051379:Mice; D008810:Mice, Inbred C57BL; D010190:Pancreatic Neoplasms / Q000276:immunology*; D010641:Phenotype; D012463:Saliva / Q000276:immunology*
chemical_list: D007155:Immunologic Factors
keywords: cancer; extracellular vesicles; immune surveillance