title: SWATH-MS dataset of heat-shock treatedDrosophila melanogasterembryos.
abstract: Data independent acquisition (DIA) has emerged as a promising mass spectrometry based approach, combining the advantages of shotgun and targeted proteomics. Here we applied a DIA approach (termed SWATH) to monitor the dynamics of theDrosophila melanogasterembryonic proteome upon heat-shock treatment. Embryos were incubated for 0.5, 1 or 3 h at 37 °C to induce heat-shock or maintained at 25 °C. The present dataset contains SWATH files acquired on a Sciex Triple-TOF 6600. A spectral library built in-house was used to analyse these data and led to the quantification of more than 2500 proteins at every timepoint. The files presented here are permanent digital maps and can be reanalysed to search for new questions. The data have been deposited with the ProteomeXchange Consortium with the dataset identifier PRIDE: PXD004753.
authors.affiliation: Cambridge Centre for Proteomics, Department of Biochemistry, University of Cambridge, Cambridge, U.K; Department of Biochemistry, University of Cambridge, Cambridge, U.K; Cambridge Systems Biology Centre, University of Cambridge, Cambridge, U.K.
authors.forename: Bertrand
authors.initials: B
authors.lastname: Fabre
authors.affiliation: Department of Genetics, University of Cambridge, Cambridge, U.K; Cambridge Systems Biology Centre, University of Cambridge, Cambridge, U.K.
authors.forename: Dagmara
authors.initials: D
authors.lastname: Korona
authors.affiliation: Cambridge Centre for Proteomics, Department of Biochemistry, University of Cambridge, Cambridge, U.K; Department of Biochemistry, University of Cambridge, Cambridge, U.K; Cambridge Systems Biology Centre, University of Cambridge, Cambridge, U.K.
authors.forename: Daniel J H
authors.initials: DJ
authors.lastname: Nightingale
authors.affiliation: Department of Genetics, University of Cambridge, Cambridge, U.K; Cambridge Systems Biology Centre, University of Cambridge, Cambridge, U.K.
authors.forename: Steven
authors.initials: S
authors.lastname: Russell
authors.affiliation: Cambridge Centre for Proteomics, Department of Biochemistry, University of Cambridge, Cambridge, U.K; Department of Biochemistry, University of Cambridge, Cambridge, U.K; Cambridge Systems Biology Centre, University of Cambridge, Cambridge, U.K.
authors.forename: Kathryn S
authors.initials: KS
authors.lastname: Lilley
keywords: Drosophila melanogaster; Heat shock; Mass-spectrometry; SWATH