title: Accuracy of the evaluation method for alkaline agents' bactericidal efficacies in solid, and the required time of bacterial inactivation.
abstract: An alkaline agent, namely food additive grade calcium hydroxide (FdCa (OH)2) in the powder form, was evaluated for its bactericidal efficacies in chicken feces at pH 13. The point for this evaluation was neutralization of the alkaline agent's pH at the time of bacterial recovery, since otherwise the results are substantially misleading. Without neutralization of the FdCa (OH)2pH, the spiked bacteria were killed within min at the time of recovery in aqueous phase, but not in the solid form in feces, hence, it has been demonstrated that when bacteria were in solid, it took longer time than in liquid for the alkaline agent to inactivate them down to the acceptable level (≥3 log10CFU/ml).
authors.affiliation: Laboratory of Animal Health, Department of Veterinary Medicine, Faculty of Agriculture, Tokyo University of Agriculture and Technology, 3-5-8, Saiwai-cho, Fuchu-shi, Tokyo 183-8509, Japan.
authors.forename: Hakimullah
authors.initials: H
authors.lastname: Hakim
authors.forename: Chiharu
authors.initials: C
authors.lastname: Toyofuku
authors.forename: Mari
authors.initials: M
authors.lastname: Ota
authors.forename: Mayuko
authors.initials: M
authors.lastname: Suzuki
authors.forename: Miyuki
authors.initials: M
authors.lastname: Komura
authors.forename: Masashi
authors.initials: M
authors.lastname: Yamada
authors.forename: Md Shahin
authors.initials: MS
authors.lastname: Alam
authors.forename: Natthanan
authors.initials: N
authors.lastname: Sangsriratanakul
authors.forename: Dany
authors.initials: D
authors.lastname: Shoham
authors.forename: Kazuaki
authors.initials: K
authors.lastname: Takehara
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002126:Calcium Hydroxide / Q000494:pharmacology*; D002645:Chickens; D004926:Escherichia coli / Q000187:drug effects*; D005243:Feces / Q000382:microbiology; D005503:Food Additives / Q000494:pharmacology*; D006863:Hydrogen-Ion Concentration; D012475:Salmonella / Q000187:drug effects*
chemical_list: D000900:Anti-Bacterial Agents; D005503:Food Additives; D002126:Calcium Hydroxide