title: Community-Metabolome Correlations of Gut Microbiota from Child-Turcotte-Pugh of A and B Patients.
abstract: The gut flora are widely involved in the cometabolism with the host and have evident effects on the metabolic phenotype of host. This study performed a metabolome analysis of the intestinal microbiota specific for liver cirrhosis. The study population included patients with Child-Turcotte-Pugh score of A (AP,n= 5) and B (BP,n= 5), and control subjects (NM,n= 3). Metagenomic DNA from fecal microbiota was extracted followed by metagenomic sequencing through Illumina MiSeq high throughput sequencing of 16S rRNA regions. The detection of metabolites from fecal samples was performed using high-performance liquid phase chromatography and gas chromatography coupled with tandem mass spectrometry. Intestinal microbiota community and metabolite analysis both showed separation of cirrhotic patients from control participants, moreover, the microbiota-metabolite correlations changed in cirrhotic patients. Fecal microbiota from cirrhotic patients, with the reduced diversity, contained a decreased abundance of Bacteroidetes and an increased abundance of Firmicutes and Proteobacteria compared with the normal samples. Analysis of metabolome revealed a remarkable change in the metabolic potential of the microbiota in cirrhotic patients, with specific higher concentrations of amine, unsaturated fatty acid, and short-chain fatty acids, and lower concentrations of sugar alcohol and amino acid, suggesting the initial equilibrium of gut microbiota community and co-metabolism with the host were perturbed by cirrhosis. Our study illustrated the relationship between fecal microbiota composition and metabolome in cirrhotic patients, which may improve the clinical prognosis of cirrhosis.
authors.affiliation: Institute of Disease Control and Prevention, Academy of Military Medical Sciences, Fengtai District Beijing, China.
authors.forename: Xiao
authors.initials: X
authors.lastname: Wei
authors.affiliation: Institute of Disease Control and Prevention, Academy of Military Medical Sciences, Fengtai District Beijing, China.
authors.forename: Shan
authors.initials: S
authors.lastname: Jiang
authors.affiliation: Institute of Disease Control and Prevention, Academy of Military Medical Sciences, Fengtai District Beijing, China.
authors.forename: Xiangna
authors.initials: X
authors.lastname: Zhao
authors.affiliation: Institute of Disease Control and Prevention, Academy of Military Medical Sciences, Fengtai District Beijing, China.
authors.forename: Huan
authors.initials: H
authors.lastname: Li
authors.affiliation: Institute of Disease Control and Prevention, Academy of Military Medical Sciences, Fengtai District Beijing, China.
authors.forename: Weishi
authors.initials: W
authors.lastname: Lin
authors.affiliation: Institute of Disease Control and Prevention, Academy of Military Medical Sciences, Fengtai District Beijing, China.
authors.forename: Boxing
authors.initials: B
authors.lastname: Li
authors.affiliation: Institute of Disease Control and Prevention, Academy of Military Medical Sciences, Fengtai District Beijing, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Lu
authors.affiliation: Institute of Disease Control and Prevention, Academy of Military Medical Sciences, Fengtai District Beijing, China.
authors.forename: Yansong
authors.initials: Y
authors.lastname: Sun
authors.affiliation: Institute of Disease Control and Prevention, Academy of Military Medical Sciences, Fengtai District Beijing, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Yuan
keywords: SCFAs; amino acid; gut microbiota; liver cirrhosis; metabolome