title: Extraction of Opuntia dillenii Haw. Polysaccharides and Their Antioxidant Activities.
abstract: Use of natural polysaccharides in medicine and food has wide interest in research. In this study, we extracted and purified some polysaccharides from cactusOpuntia dilleniiHaw. (ODP). Some preliminary functions of these products were characterized. Under the optimal purification conditions, the yield of ODP extracted from the 2-4 month-oldOpuntia dilleniiHaw. (T-ODP) was 30.60% ± 0.40%, higher than that of ODP from the 5-10 month-old materials (O-ODP) (18.97% ± 0.58%). The extracted ODP was purified by DEAE sepharose fast flow anion exchange and Sephacryl S-400 chromatography with four fractions obtained (ODP-Ia, ODP-Ib, ODP-IIa and ODP-IIb). Analysis with UV-vis chromatography indicated that ODP-Ia and ODP-IIa were relatively homogeneous molecules with a molecular weight of 339 kD and 943 kD, respectively. Results of infrared spectroscopy indicated that ODP, ODP-Ia, and ODP-IIa were acidic polysaccharides. Further, the antioxidant activity against DPPH (1,1-diphenyl-2-picrylhydrazyl) radical, hydroxyl radicals, and superoxide radical in vitro demonstrated that the T-ODP exhibited higher antioxidant activity than the O-ODP, and the purified fraction (ODP-Ia) was superior to the ODP. These results will offer a theoretical basis for further research on the structure-function relationship of ODP and the rational utilization ofOpuntia dilleniiHaw.
authors.affiliation: College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha 410128, China. liheng80@126.com.|School of Life Science and Technology, Lingnan Normal University, Zhanjiang 524048, China. liheng80@126.com.
authors.forename: Heng
authors.initials: H
authors.lastname: Li
authors.affiliation: School of Pharmacy, South-Central University for Nationalities, Wuhan 430074, China. qingxiayuan@mail.scuec.edu.cn.
authors.forename: Qingxia
authors.initials: Q
authors.lastname: Yuan
authors.affiliation: School of Life Science and Technology, Lingnan Normal University, Zhanjiang 524048, China. zhouxianjiao@163.com.
authors.forename: Xianjiao
authors.initials: X
authors.lastname: Zhou
authors.affiliation: School of Life Science and Technology, Lingnan Normal University, Zhanjiang 524048, China. zzengfuhua@163.com.|Engineering Center of Resource Plant, Zhanjiang 524048, China. zzengfuhua@163.com.
authors.forename: Fuhua
authors.initials: F
authors.lastname: Zeng
authors.affiliation: College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha 410128, China. xiangyangcn@163.com.
authors.forename: Xiangyang
authors.initials: X
authors.lastname: Lu
mesh_terms: D000975:Antioxidants / Q000737:chemistry*; D001713:Biphenyl Compounds / Q000737:chemistry; D017665:Hydroxyl Radical / Q000737:chemistry; D029747:Opuntia / Q000737:chemistry*; D010851:Picrates / Q000737:chemistry; D010936:Plant Extracts / Q000737:chemistry*; D011134:Polysaccharides / Q000737:chemistry*; D013481:Superoxides / Q000737:chemistry
chemical_list: D000975:Antioxidants; D001713:Biphenyl Compounds; D010851:Picrates; D010936:Plant Extracts; D011134:Polysaccharides; D013481:Superoxides; D017665:Hydroxyl Radical; C004931:1,1-diphenyl-2-picrylhydrazyl
keywords: Opuntia dillenii Haw.; antioxidation; characterization; extraction; polysaccharide