title: Structure-cytotoxicity relationship of methacrylate-based resin monomers as evaluated by an anti-oxidant responsive element-luciferase reporter assay.
abstract: To investigate the chemical structure-cytotoxicity relationship of methacrylate-based resin monomers, we studied their effects on anti-oxidant responsive element (ARE)-mediated transcription. HepG2 cells stably expressing an ARE-regulated luciferase reporter gene were cultured for 6 h with various concentrations of several resin monomers and subjected to a luciferase assay. The doseresponse curves observed for hydrophobic monomers with different hydrocarbon chains (MMA, EMA, PMA and BMA) began to rise at concentrations between 0.5 and 1 mM; the curves rose as the monomer concentrations increased up to 5 (BMA), 10 (PMA), or 30 mM (MMA and EMA). In contrast, hydrophilic monomers having a hydroxyl group (HEMA and HPMA) showed bell-shaped curves, and stimulated the reporter expression more strongly than the hydrophobic monomers in a low concentration range (0.5-5 mM). The results suggest that introduction of a hydroxyl group in a methacrylate-based resin monomer increases its intracellular electrophilic reactivity and cytotoxicity.
authors.affiliation: Department of Dental Material Science, School of Dentistry, Aichi-Gakuin University.
authors.forename: Miki
authors.initials: M
authors.lastname: Egashira
authors.forename: Takahiro
authors.initials: T
authors.lastname: Suzuki
authors.forename: Ai
authors.initials: A
authors.lastname: Orimoto
authors.forename: Tohru
authors.initials: T
authors.lastname: Obata
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Nakamura
authors.forename: Motohiro
authors.initials: M
authors.lastname: Tanaka
authors.forename: Takao
authors.initials: T
authors.lastname: Kanamori
authors.forename: Tatsushi
authors.initials: T
authors.lastname: Kawai
mesh_terms: D005347:Fibroblasts; D017930:Genes, Reporter; D006801:Humans; D008156:Luciferases; D008689:Methacrylates / Q000633:toxicity*; D016877:Oxidants; D012117:Resins, Synthetic
chemical_list: D008689:Methacrylates; D016877:Oxidants; D012117:Resins, Synthetic; D008156:Luciferases