title: Libkindia masarykiana gen. et sp. nov., Yurkovia mendeliana gen. et sp. nov. and Leucosporidium krtinense f.a. sp. nov., isolated from temperate forest soils.
abstract: One hundred and ninety-eight isolates of soil yeasts were isolated from mixed temperate forests in the Czech Republic, and their abundance and distribution in the litter and soil were evaluated using amplicon sequencing of soil fungal communities. Abundant taxa with no close identified hits were selected for further characterization as potential novel species of yeasts. Phylogenetic analyses using sequences of the D1/D2 domain, the ITS region and RPB1 and TEF1 genes support the recognition of the following three novel species belonging to the subphylum Pucciniomycotina, class Microbotryomycetes: Leucosporidium krtinense f.a. sp. nov. (type strain PYCC 6879T=KT96T=CBS 14304T=DSM 101892T), Yurkovia mendeliana sp. nov. (type strain PYCC 6884T=KT152T=CBS 14273T=DSM 101889T) and Libkindia masarykiana sp. nov. (type strain PYCC 6886T=KT310T=CBS 14275T=DSM 101891T). Since the latter two novel taxa cannot be assigned to existing genera, two new genera, Libkindia gen. nov. and Yurkovia gen. nov., are also described.
authors.affiliation: Laboratory of Environmental Microbiology, Institute of Microbiology of the CAS, Vídeňská 1083, 14220 Praha 4, Czech Republic.
authors.forename: Tereza
authors.initials: T
authors.lastname: Mašínová
authors.affiliation: UCIBIO-REQUIMTE, Departamento de Ciências da Vida, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, 2829-516 Caparica, Portugal.
authors.forename: Ana
authors.initials: A
authors.lastname: Pontes
authors.affiliation: UCIBIO-REQUIMTE, Departamento de Ciências da Vida, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, 2829-516 Caparica, Portugal.
authors.forename: Cláudia
authors.initials: C
authors.lastname: Carvalho
authors.affiliation: UCIBIO-REQUIMTE, Departamento de Ciências da Vida, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, 2829-516 Caparica, Portugal.
authors.forename: José Paulo
authors.initials: JP
authors.lastname: Sampaio
authors.affiliation: Laboratory of Environmental Microbiology, Institute of Microbiology of the CAS, Vídeňská 1083, 14220 Praha 4, Czech Republic.
authors.forename: Petr
authors.initials: P
authors.lastname: Baldrian
mesh_terms: D001482:Base Composition; D001487:Basidiomycota / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D018153:Czech Republic; D004271:DNA, Fungal / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000235:genetics; D065928:Forests; D005800:Genes, Fungal; D010802:Phylogeny; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology
chemical_list: D004271:DNA, Fungal; D021903:DNA, Ribosomal Spacer