title: Corticicoccus populi gen. nov., sp. nov., a member of the family Staphylococcaceae, isolated from symptomatic bark of Populus × euramericana canker.
abstract: Two Gram-stain-positive, aerobic, non-motile, bacterial strains were isolated from symptomatic bark tissue of Populus × euramericana canker. The isolates were able to grow between 10 and 37 °C, at pH 6-10 and with 0-4 % (w/v) NaCl, with optimal growth at 28-30 °C, pH 7.0-8.0 and with 2 % (w/v) NaCl The strains were found to be oxidase and catalase positive. The menaquinone of strain 26D10-3-4T was MK-7 and the peptidoglycan type A3α based on l-Lys-Gly3-?Ala. The polar lipid profiles of strain 26D10-3-4T showed diphosphatidylglycerol, phosphatidylglycerol, two unidentified phospholipids and three unidentified glycolipids, and the major fatty acids found were anteiso-C15 : 0, iso-C15 : 0, iso-C17 : 0 and anteiso-C17 : 0. The DNA G+C content was 38.2 mol%. The two novel isolates shared the highest 16S rRNA gene sequence similarity with Salinicoccus qingdaonensis ZXM223T (95.0 %). Based on phenotypic and genotypic characteristics, these two strains represent a novel species of a new genus of the family Staphylococcaceae; the name Corticicoccus populi gen. nov., sp. nov. is proposed. The type strain of the type species is 26D10-3-4T (=CFCC 12725T=KCTC 33575T). An additional strain of the species is 9-4-1.
authors.affiliation: The Key Laboratory of State Forestry Administration on Forest Protection, Research Institute of Forest Ecology Environment and Protection, Chinese Academy of Forestry, Beijing 100091, PR China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Li
authors.affiliation: Research Institute of Tropical Forestry, Chinese Academy of Forestry, Guangzhou 510520, PR China.
authors.forename: Sheng-Kun
authors.initials: SK
authors.lastname: Wang
authors.affiliation: The Key Laboratory of State Forestry Administration on Forest Protection, Research Institute of Forest Ecology Environment and Protection, Chinese Academy of Forestry, Beijing 100091, PR China.
authors.forename: Han
authors.initials: H
authors.lastname: Xue
authors.affiliation: Puyang Academy of Forestry, Puyang 457000, PR China.
authors.forename: Ju-Pu
authors.initials: JP
authors.lastname: Chang
authors.affiliation: Puyang Academy of Forestry, Puyang 457000, PR China.
authors.forename: Li-Min
authors.initials: LM
authors.lastname: Guo
authors.affiliation: Puyang Academy of Forestry, Puyang 457000, PR China.
authors.forename: Xu-Qi
authors.initials: XQ
authors.lastname: Yang
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D002473:Cell Wall / Q000737:chemistry; D002681:China; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D006017:Glycolipids / Q000737:chemistry; D010457:Peptidoglycan / Q000737:chemistry; D010743:Phospholipids / Q000737:chemistry; D010802:Phylogeny; D024301:Plant Bark / Q000382:microbiology*; D010935:Plant Diseases / Q000382:microbiology*; D032107:Populus / Q000382:microbiology*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D040141:Staphylococcaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D006017:Glycolipids; D010457:Peptidoglycan; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; C062629:menaquinone 7