title: A combined linkage and association strategy identifies a variant near the GSTP1 gene associated with BMI in the Mexican population.
abstract: Obesity is a major public health concern in Mexico and worldwide. Although the estimated heritability is high, common variants identified by genome-wide association studies explain only a small proportion of this heritability. A combination of linkage and association strategies could be a more robust and powerful approach to identify other obesity-susceptibility variants. We thus sought to identify novel genetic variants associated with obesity-related traits in the Mexican population by combining these methods. We performed a genome-wide linkage scan for body mass index (BMI) and other obesity-related phenotypes in 16 Mexican families using the Sequential Oligogenic Linkage Analysis Routines Program. Associated single-nucleotide polymorphisms (SNPs) were tested for associations in an independent cohort. Two suggestive BMI-linkage peaks (logarithm of odds ⩾1.5) were observed at chromosomal regions 11q13 and 13q22. Only rs614080 in the 11q13 region was significantly associated with BMI and related traits in these families. This association was also significant in an independent cohort of Mexican adults. Moreover, this variant was significantly associated with GSTP1 gene expression levels in adipose tissue. In conclusion, the rs614080 SNP near the GSTP1 gene was significantly associated with BMI and GSTP1 expression levels in the Mexican population.
authors.affiliation: Programa de Doctorado en Ciencias Biológicas y de la Salud, Universidad Autónoma Metropolitana, México City, México.|Unidad de Genómica de Poblaciones Aplicada a la Salud, Facultad de Química, UNAM/Instituto Nacional de Medicina Genómica (INMEGEN), México City, México.
authors.forename: Hugo
authors.initials: H
authors.lastname: Villamil-Ramírez
authors.affiliation: Unidad de Genómica de Poblaciones Aplicada a la Salud, Facultad de Química, UNAM/Instituto Nacional de Medicina Genómica (INMEGEN), México City, México.
authors.forename: Paola
authors.initials: P
authors.lastname: León-Mimila
authors.affiliation: Unidad de Genómica de Poblaciones Aplicada a la Salud, Facultad de Química, UNAM/Instituto Nacional de Medicina Genómica (INMEGEN), México City, México.
authors.forename: Luis R
authors.initials: LR
authors.lastname: Macias-Kauffer
authors.affiliation: Facultad de Medicina, Universidad Autónoma de Sinaloa, Sinaloa, México.
authors.forename: Adrián
authors.initials: A
authors.lastname: Canizalez-Román
authors.affiliation: Departamento de Genómica Computacional, INMEGEN, México City, México.
authors.forename: Marisela
authors.initials: M
authors.lastname: Villalobos-Comparán
authors.affiliation: Facultad de Medicina, Universidad Autónoma de Sinaloa, Sinaloa, México.
authors.forename: Nidia
authors.initials: N
authors.lastname: León-Sicairos
authors.affiliation: Unidad de Genómica de Poblaciones Aplicada a la Salud, Facultad de Química, UNAM/Instituto Nacional de Medicina Genómica (INMEGEN), México City, México.
authors.forename: Joel
authors.initials: J
authors.lastname: Vega-Badillo
authors.affiliation: Departamento de Inmunología, Instituto Nacional de Cardiología Ignacio Chávez (INCICh), México City, México.
authors.forename: Fausto
authors.initials: F
authors.lastname: Sánchez-Muñoz
authors.affiliation: Unidad de Genómica de Poblaciones Aplicada a la Salud, Facultad de Química, UNAM/Instituto Nacional de Medicina Genómica (INMEGEN), México City, México.
authors.forename: Blanca
authors.initials: B
authors.lastname: López-Contreras
authors.affiliation: Unidad de Genómica de Poblaciones Aplicada a la Salud, Facultad de Química, UNAM/Instituto Nacional de Medicina Genómica (INMEGEN), México City, México.
authors.forename: Sofía
authors.initials: S
authors.lastname: Morán-Ramos
authors.affiliation: Laboratorio de Enfermedades Cardiovasculares, INMEGEN, México City, México.
authors.forename: Teresa
authors.initials: T
authors.lastname: Villarreal-Molina
authors.affiliation: Clínica Integral de Cirugía para la Obesidad y Enfermedades Metabólicas, Hospital General 'Dr Rubén Leñero', México City, México.
authors.forename: Luis C
authors.initials: LC
authors.lastname: Zurita
authors.affiliation: Clínica Integral de Cirugía para la Obesidad y Enfermedades Metabólicas, Hospital General 'Dr Rubén Leñero', México City, México.
authors.forename: Francisco
authors.initials: F
authors.lastname: Campos-Pérez
authors.affiliation: Department of Medicine, David Geffen School of Medicine, UCLA, Los Angeles, CA, USA.
authors.forename: Adriana
authors.initials: A
authors.lastname: Huertas-Vazquez
authors.affiliation: Departamento de Inmunología, Instituto Nacional de Cardiología Ignacio Chávez (INCICh), México City, México.|Departmento de Atención a la salud, Universidad Autónoma Metropolitana-Xochimilco, México City, México.
authors.forename: Rafael
authors.initials: R
authors.lastname: Bojalil
authors.affiliation: Departamento de Genómica Computacional, INMEGEN, México City, México.
authors.forename: Sandra
authors.initials: S
authors.lastname: Romero-Hidalgo
authors.affiliation: Departamento de Endocrinología y Metabolismo, Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán, México City, México.
authors.forename: Carlos A
authors.initials: CA
authors.lastname: Aguilar-Salinas
authors.affiliation: Unidad de Genómica de Poblaciones Aplicada a la Salud, Facultad de Química, UNAM/Instituto Nacional de Medicina Genómica (INMEGEN), México City, México.
authors.forename: Samuel
authors.initials: S
authors.lastname: Canizales-Quinteros
mesh_terms: D000273:Adipose Tissue / Q000378:metabolism / Q000473:pathology; D000293:Adolescent; D000328:Adult; D000368:Aged; D015992:Body Mass Index; D002880:Chromosomes, Human, Pair 11 / Q000737:chemistry; D005190:Family; D005260:Female; D008040:Genetic Linkage; D020022:Genetic Predisposition to Disease; D055106:Genome-Wide Association Study; D051549:Glutathione S-Transferase pi / Q000235:genetics*; D006801:Humans; D040582:Inheritance Patterns; D008297:Male; D008800:Mexico / Q000453:epidemiology; D008875:Middle Aged; D009765:Obesity / Q000453:epidemiology / Q000235:genetics* / Q000473:pathology; D020641:Polymorphism, Single Nucleotide; D040641:Quantitative Trait Loci; D019655:Quantitative Trait, Heritable
chemical_list: C496556:GSTP1 protein, human; D051549:Glutathione S-Transferase pi