title: Screening Mammalian Cells on a Hydrogel: Functionalized Small Molecule Microarray.
abstract: Mammalian cell-based microarray technology has gained wide attention, for its plethora of promising applications. The platform is able to provide simultaneous information on multiple parameters for a given target, or even multiple target proteins, in a complex biological system. Here we describe the preparation of mammalian cell-based microarrays using selectively captured of human prostate cancer cells (PC-3). This platform was then used in controlled drug release and measuring the associated drug effects on these cancer cells.
authors.affiliation: Department of Chemistry, National University of Singapore, 3 Science Drive 3, Singapore, 117543, Singapore.
authors.forename: Biwei
authors.initials: B
authors.lastname: Zhu
authors.affiliation: Department of Chemistry, National University of Singapore, 3 Science Drive 3, Singapore, 117543, Singapore.
authors.forename: Bo
authors.initials: B
authors.lastname: Jiang
authors.affiliation: Department of Chemistry, National University of Singapore, 3 Science Drive 3, Singapore, 117543, Singapore.
authors.forename: Zhenkun
authors.initials: Z
authors.lastname: Na
authors.affiliation: Department of Chemistry, Faculty of Science, National University of Singapore, 3 Science Drive 3, Singapore, 117543, Singapore. chmyaosq@nus.edu.sg.
authors.forename: Shao Q
authors.initials: SQ
authors.lastname: Yao
mesh_terms: D000818:Animals; D002166:Camptothecin / Q000494:pharmacology; D002460:Cell Line; D048430:Cell Shape / Q000187:drug effects; D005453:Fluorescence; D006801:Humans; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000737:chemistry*; D008322:Mammals; D046228:Microarray Analysis / Q000379:methods*; D010455:Peptides / Q000138:chemical synthesis / Q000737:chemistry; D054852:Small Molecule Libraries / Q000032:analysis*
chemical_list: D010455:Peptides; D054852:Small Molecule Libraries; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D002166:Camptothecin
keywords: Bio-imaging; Cancer; Controlled drug release; Hydrogel; Mammalian cell-based microarray