title: Fluoro-edenite induces fibulin-3 overexpression in non-malignant human mesothelial cells.
abstract: Exposure to asbestos is associated with the development of mesothelioma. In addition to asbestos, other fibers have been identified as risk factors for malignant and non-malignant diseases of the lungs. Among these, fluoro-edenite (FE) was found in patients from Biancavilla (Sicily, Italy) with pleural and lung disease, suggesting its role for tumor expansion. In this context, the identification of early biomarkers useful for the diagnosis of cancer is mandatory. Fibulin-3 represents an important marker for the diagnosis of mesothelioma. However, it remains to be determined whether it is directly associated with exposure to asbestos-like fibers. In the present study, peripheral blood levels of fibulin-3 from 40 asbestos-exposed workers were compared with those detected in 27 street cleaners from Biancavilla. Intriguingly, the results showed that fibulin-3 levels were higher in the group of street cleaners compared with those of the asbestos-exposed workers, suggesting that these workers used the personal protective equipment according to the current regulations. These data suggest that subjects exposed to FE should be monitored for the risk of mesothelioma. FE and volcanic particulates are probably contained within dust inhaled by street cleaners from Biancavilla during their work activities. Based on these criteria, in this study, such fibers were used to treat mesothelial cells (MeT5A) in order to verify whether fibulin-3 levels are affected by these treatments. The results showed that only treatment with FE was associated with fibulin-3 overexpression at both the transcript and protein levels. It was previously demonstrated that mesothelial cells exhibited low levels of p27 following treatment with FE. Notably, p27 downregulation is associated with stathmin upregulation in cancer, conferring an aggressive phenotype of tumor cells. This observation prompted us to perform a computational evaluation demonstrating the activation of stathmin in lung cancer in patients exposed to asbestos. Overall, it can be speculated that both fibulin-3 and stathmin overexpression may be associated with the malignant transformation of mesothelial cells following exposure to asbestos-like fibers.
authors.affiliation: Section of Occupational Medicine, Department of Clinical and Experimental Medicine, University of Catania, I-95124 Catania, Italy.
authors.forename: Venerando
authors.initials: V
authors.lastname: Rapisarda
authors.affiliation: Section of General and Clinical Pathology and Oncology, Department of Biomedical and Biotechnological Sciences, University of Catania, I-95124 Catania, Italy.
authors.forename: Rossella
authors.initials: R
authors.lastname: Salemi
authors.affiliation: Section of Occupational Medicine, Department of Clinical and Experimental Medicine, University of Catania, I-95124 Catania, Italy.
authors.forename: Andrea
authors.initials: A
authors.lastname: Marconi
authors.affiliation: Section of Human Anatomy and Histology, Department of Biomedical and Biotechnological Sciences, University of Catania, I-95124 Catania, Italy.
authors.forename: Carla
authors.initials: C
authors.lastname: Loreto
authors.affiliation: Section of Physiology, Department of Biomedical and Biotechnological Sciences, University of Catania, I-95124 Catania, Italy.
authors.forename: Adriana C
authors.initials: AC
authors.lastname: Graziano
authors.affiliation: Section of Physiology, Department of Biomedical and Biotechnological Sciences, University of Catania, I-95124 Catania, Italy.
authors.forename: Venera
authors.initials: V
authors.lastname: Cardile
authors.affiliation: Section of General and Clinical Pathology and Oncology, Department of Biomedical and Biotechnological Sciences, University of Catania, I-95124 Catania, Italy.
authors.forename: Maria S
authors.initials: MS
authors.lastname: Basile
authors.affiliation: Section of General and Clinical Pathology and Oncology, Department of Biomedical and Biotechnological Sciences, University of Catania, I-95124 Catania, Italy.
authors.forename: Saverio
authors.initials: S
authors.lastname: Candido
authors.affiliation: Section of General and Clinical Pathology and Oncology, Department of Biomedical and Biotechnological Sciences, University of Catania, I-95124 Catania, Italy.
authors.forename: Luca
authors.initials: L
authors.lastname: Falzone
authors.affiliation: Department of Virology, Faculty of Medicine, University of Crete, 71003 Heraklion, Crete, Greece.
authors.forename: Demetrios A
authors.initials: DA
authors.lastname: Spandidos
authors.affiliation: Section of Occupational Medicine, Department of the Environment, Safety, Territory, Food and Health Sciences, University of Messina, I-98125 Messina, Italy.
authors.forename: Concettina
authors.initials: C
authors.lastname: Fenga
authors.affiliation: Section of General and Clinical Pathology and Oncology, Department of Biomedical and Biotechnological Sciences, University of Catania, I-95124 Catania, Italy.
authors.forename: Massimo
authors.initials: M
authors.lastname: Libra
keywords: asbestos; fibulin-3; fluoro-edentite; lung cancer; mesothelioma; stathmin