title: Downregulated miR-486-5p acts as a tumor suppressor in esophageal squamous cell carcinoma.
abstract: microRNAs (miRNAs/miRs) are crucial regulators of gene expression at the post-translational level through promoting mRNA degradation or the repression of translation of target genes. miRs have been confirmed to serve a dominant role in tumor biology. miR-486-5p has been ascertained to be involved in non-small-cell lung cancer, breast cancer and hepatocellular carcinoma; however, the expression and function of miR-486-5p in esophageal squamous cell carcinoma (ESCC) has yet to be elucidated. The present study aimed to analyze the expression levels of miR-486-5p in ESCC tissues and paired normal adjacent tissues, and determine the effects of miR-486-5p on esophageal cancer cells using MTT, wound scratch and apoptosis assays. The current results showed that miR-486-5p was significantly downregulated in ESCC specimens. Ectopic expression of miR-486-5p by synthetic mimics reduced cell proliferation and migration and induced increased cell apoptosis. The results indicated miR-486-5p may function as a tumor suppressor in ESCC. The present study demonstrated that miR-486-5p was downregulated in ESCC and served a anti-oncogene role in ESCC via affecting cellular migration.
authors.affiliation: Department of Thoracic Surgery, Dongnan Affiliated Hospital of Xiamen University, Zhangzhou, Fujian 363000, P.R. China.
authors.forename: Yunfeng
authors.initials: Y
authors.lastname: Yi
authors.affiliation: Department of Thoracic Surgery, Dongnan Affiliated Hospital of Xiamen University, Zhangzhou, Fujian 363000, P.R. China.
authors.forename: Xiujuan
authors.initials: X
authors.lastname: Lu
authors.affiliation: Department of Thoracic Surgery, Dongnan Affiliated Hospital of Xiamen University, Zhangzhou, Fujian 363000, P.R. China.
authors.forename: Jianming
authors.initials: J
authors.lastname: Chen
authors.affiliation: Department of Thoracic Surgery, Dongnan Affiliated Hospital of Xiamen University, Zhangzhou, Fujian 363000, P.R. China.
authors.forename: Changjie
authors.initials: C
authors.lastname: Jiao
authors.affiliation: Department of Thoracic Surgery, Dongnan Affiliated Hospital of Xiamen University, Zhangzhou, Fujian 363000, P.R. China.
authors.forename: Jing
authors.initials: J
authors.lastname: Zhong
authors.affiliation: Department of Thoracic Surgery, Dongnan Affiliated Hospital of Xiamen University, Zhangzhou, Fujian 363000, P.R. China.
authors.forename: Zhiming
authors.initials: Z
authors.lastname: Song
authors.affiliation: Department of Thoracic Surgery, Dongnan Affiliated Hospital of Xiamen University, Zhangzhou, Fujian 363000, P.R. China.
authors.forename: Xiaoping
authors.initials: X
authors.lastname: Yu
authors.affiliation: Department of Thoracic Surgery, Dongnan Affiliated Hospital of Xiamen University, Zhangzhou, Fujian 363000, P.R. China.
authors.forename: Baoli
authors.initials: B
authors.lastname: Lin
keywords: downregulated; esophageal squamous cell carcinoma; miR-486-5p; tumor suppressor