title: Blood Bio-Sampling Procedures for Multiplex Biomarkers Studies.
abstract: A major challenge in single or panel of biomarker discovery and validation is the inherent biological complexity underlying disease heterogeneity and inconsistent responses to treatment. Moreover, the lack of standardization in the sampling, processing, and storage of biological fluids such as plasma and serum disrupts the discovery and validation of blood-based biomarker tests in preclinical and clinical settings. This chapter presents a reproducible sample collection and handling procedure that aims to enhance analyte stability and ensure compatibility with the corresponding multiplex biomarker profiling platforms. The importance of defining bio-sample acquisition and processing, study design, and profiling platform guidelines for blood-based biomarker measurements is paramount for the success of personalized healthcare strategy and development of companion diagnostics.
authors.affiliation: Laboratory of Neuroproteomics, Department of Biochemistry and Tissue Biology, Institute of Biology, University of Campinas (UNICAMP), Rua Monteiro Lobato 255 F/01, Cidade Universitária ZeferinoVaz, 13083-862, Campinas, Brazil.
authors.forename: Paul C
authors.initials: PC
authors.lastname: Guest
authors.affiliation: Department of Chemical Engineering and Biotechnology, University of Cambridge, CB2 3RA, Cambridge, UK. hr228@cam.ac.uk.
authors.forename: Hassan
authors.initials: H
authors.lastname: Rahmoune
mesh_terms: D015415:Biomarkers / Q000097:blood*; D001800:Blood Specimen Collection / Q000379:methods; D006801:Humans; D055432:Metabolomics / Q000379:methods; D010949:Plasma; D040901:Proteomics / Q000379:methods; D044967:Serum; D013048:Specimen Handling / Q000379:methods
chemical_list: D015415:Biomarkers
keywords: Bio-processing; Bio-sampling; Biomarkers; Plasma; Serum