title: Neurotrophic factor mediated neuronal differentiation of human cord blood mesenchymal stem cells and their applicability to assess the developmental neurotoxicity.
abstract: Plasticity and developmental capacity of stem cells have now been established as a promising tool to restore the degenerative disorders. The linearity differentiation of human mesenchymal stem cells (hMSCs) into adipogenic, chondrogenic, osteogenic and even in neuronal subtypes has been demonstrated. The number of xenobiotics such as dexamethasone, insulin, isobutyl 1-methyle xanthine and retinoic acid has been reported for the potential to differentiate hMSCs into neuronal subtypes. But, the applicability of indigenous neurotrophic factor-nerve growth factor (NGF) has not been explored for the purpose. Thus, the present investigations were carried out to study the NGF induced neuronal differentiation of hMSCs. Following the isolation, purification and characterization of hMSCs were allowed to differentiate into neuronal subtypes under the influence of NGF (50 ng/mL). At various concentrations of NGF, the neuronal makers were analysed at both mRNA and protein levels. Cells, exposed with NGF were showing the significant and gradual increase in the neuronal markers in differentiating cells. The magnitude of expression of markers was maximum at day 4 of differentiation. NGF at 50 ng/mL concentration was found to induce neuronal differentiation of hMSCs into neuronal subtypes.
authors.affiliation: System Toxicology and Health Risk Assessment Group, CSIR-Indian Institute of Toxicology Research, 31, VishvigyanBhawan, MG Marg, Lucknow 226001, UP, India; AcSIR Coordinator Office, Academy of Scientific and Innovative Research (AcSIR), New Delhi, India; CSIR-Central Drug Research Institute, CDRI, Jankipuram Extension, Sitapur Road, Lucknow, UP, 226031 India. Electronic address: jahan149@gmail.com.
authors.forename: Sadaf
authors.initials: S
authors.lastname: Jahan
authors.affiliation: System Toxicology and Health Risk Assessment Group, CSIR-Indian Institute of Toxicology Research, 31, VishvigyanBhawan, MG Marg, Lucknow 226001, UP, India; AcSIR Coordinator Office, Academy of Scientific and Innovative Research (AcSIR), New Delhi, India. Electronic address: thepaque@gmail.com.
authors.forename: Dipak
authors.initials: D
authors.lastname: Kumar
authors.affiliation: Career Institute of Medical Science and Associate Hospital, IIM Road, Lucknow, UP, India. Electronic address: ashvinikumar4u@gmail.com.
authors.forename: Ashvini
authors.initials: A
authors.lastname: Kumar
authors.affiliation: System Toxicology and Health Risk Assessment Group, CSIR-Indian Institute of Toxicology Research, 31, VishvigyanBhawan, MG Marg, Lucknow 226001, UP, India; AcSIR Coordinator Office, Academy of Scientific and Innovative Research (AcSIR), New Delhi, India. Electronic address: cs.rajpurohit@gmail.com.
authors.forename: Chetan Singh
authors.initials: CS
authors.lastname: Rajpurohit
authors.affiliation: System Toxicology and Health Risk Assessment Group, CSIR-Indian Institute of Toxicology Research, 31, VishvigyanBhawan, MG Marg, Lucknow 226001, UP, India; AcSIR Coordinator Office, Academy of Scientific and Innovative Research (AcSIR), New Delhi, India. Electronic address: shripriyas9@rediffmail.com.
authors.forename: Shripriya
authors.initials: S
authors.lastname: Singh
authors.affiliation: System Toxicology and Health Risk Assessment Group, CSIR-Indian Institute of Toxicology Research, 31, VishvigyanBhawan, MG Marg, Lucknow 226001, UP, India. Electronic address: aks.srvstv@gmail.com.
authors.forename: Akriti
authors.initials: A
authors.lastname: Srivastava
authors.affiliation: System Toxicology and Health Risk Assessment Group, CSIR-Indian Institute of Toxicology Research, 31, VishvigyanBhawan, MG Marg, Lucknow 226001, UP, India. Electronic address: ankitaakasomu@gmail.com.
authors.forename: Ankita
authors.initials: A
authors.lastname: Pandey
authors.affiliation: System Toxicology and Health Risk Assessment Group, CSIR-Indian Institute of Toxicology Research, 31, VishvigyanBhawan, MG Marg, Lucknow 226001, UP, India; AcSIR Coordinator Office, Academy of Scientific and Innovative Research (AcSIR), New Delhi, India. Electronic address: abpant@rediffmail.com.
authors.forename: A B
authors.initials: AB
authors.lastname: Pant
mesh_terms: D000328:Adult; D002469:Cell Separation; D002478:Cells, Cultured; D005260:Female; D005312:Fetal Blood / Q000166:cytology*; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism; D009414:Nerve Growth Factors / Q000378:metabolism*; D055495:Neurogenesis; D009474:Neurons / Q000166:cytology / Q000378:metabolism; D055815:Young Adult
chemical_list: D009414:Nerve Growth Factors
keywords: Brain; Human umbilical cord blood; Mesenchymal stem cells; Nerve growth factor; Neurodegeneration; Neuronal induction