title: Restoration of microRNA-373 suppresses growth of human T-cell lymphoma cells by repressing CCND1.
abstract: Adult T cell lymphoma is a highly aggressive T-cell malignancy. This study was designed to explore the expression and functional significance of microRNA (miR)-373 in T cell lymphoma.
  
  
  
  We analyzed the levels of CCND1 and miR-373 in T cell lymphoma tissue and the relationship of miR-373 levels with patients' prognosis. We then overexpressed miR-373 by miRNA mimics transfection and inhibited miR-373 by miRNA antisense transfection in T cell lymphoma cells. Cell survival and growth were analyzed by CCK-8 assay and MTT assay, respectively. Cell proliferation was analyzed by flow cytometry. Bioinformatics analyses were applied to predict miR-373 targets, which were then confirmed by luciferase reporter assay.
  
  
  
  We detected significantly higher levels of CCND1, and significantly lower levels of miR-373 in T cell lymphoma tissue, compared to the adjacent non-tumor tissue. Moreover, the low miR-373 levels were associated with poor survival of the patients. Overexpression of miR-373 significantly inhibited cell growth, while depletion of miR-373 increased cell growth in T cell lymphoma cells. Moreover, the effects of miR-373 on cell growth appeared to result from an alteration in cell proliferation. Finally, miR-373 was found to bind to the 3'-UTR of CCND1 mRNA to inhibit its translation in T cell lymphoma cells.
  
  
  
  Our study suggests that reduced miR-373 levels in T cell lymphoma tissue may promote T cell lymphoma growth, possibly through CCND1-mediated cell proliferation.
authors.affiliation: Department of Hematology, Harbin Medical University Cancer Hospital, Harbin, China. aichun2002@hotmail.com.
authors.forename: Y-Y
authors.initials: YY
authors.lastname: Tian
authors.forename: C-M
authors.initials: CM
authors.lastname: Jia
authors.forename: Y
authors.initials: Y
authors.lastname: Li
authors.forename: Y
authors.initials: Y
authors.lastname: Wang
authors.forename: L
authors.initials: L
authors.lastname: Jiang
authors.forename: A-C
authors.initials: AC
authors.lastname: Liu
mesh_terms: D017209:Apoptosis / Q000235:genetics; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000235:genetics; D019938:Cyclin D1; D015973:Gene Expression Regulation, Leukemic; D006801:Humans; D016399:Lymphoma, T-Cell / Q000235:genetics*; D035683:MicroRNAs / Q000235:genetics*
chemical_list: C530104:CCND1 protein, human; C527114:MIRN373 microRNA, human; D035683:MicroRNAs; D019938:Cyclin D1