title: The Expression of IGFBP6 after Spinal Cord Injury: Implications for Neuronal Apoptosis.
abstract: IGFBP6, a member of the insulin-like growth factor-binding proteins family that contains six high affinity IGFBPs, modulates insulin-like growth factor (IGF) activity and also showed an independent effect of IGF, such as growth inhibition and apoptosis. However, the role of IGFBP6 in spinal cord injury (SCI) remains largely elusive. In this study, we have performed an acute SCI model in adult rats and investigated the dynamic changes of IGFBP6 expression in the spinal cord. Our results showed that IGFBP6 was upregulated significantly after SCI, which was paralleled with the levels of apoptotic proteins p53 and active caspase-3. Immunofluorescent labeling showed that IGFBP6 was co-localizated with active caspase-3 and p53 in neurons. To further investigate the function of IGFBP6, an apoptosis model was established in primary neuronal cells. When IGFBP6 was knocked down by specific short interfering RNA (siRNA), the protein levels of active caspase-3 and Bax as well as the number of apoptotic primary neurons were significantly decreased in our study. Taken together, our findings suggest that the change of IGFBP6 protein expression plays a key role in neuronal apoptosis after SCI.
authors.affiliation: Department of Spine Surgery, The Second Affiliated Hospital of Nantong University, Nantong University, Nantong, 226001, Jiangsu Province, People's Republic of China.
authors.forename: Song
authors.initials: S
authors.lastname: Wang
authors.affiliation: Department of Pathogen Biology, Medical College, Jiangsu Province Key Laboratory for Inflammation and Molecular Drug Target, Nantong University, 19 Qi-Xiu Road, Nantong, 226001, Jiangsu Province, People's Republic of China.
authors.forename: Yonghua
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Department of Spine Surgery, The Second Affiliated Hospital of Nantong University, Nantong University, Nantong, 226001, Jiangsu Province, People's Republic of China.
authors.forename: Chunshuai
authors.initials: C
authors.lastname: Wu
authors.affiliation: Department of Gastroenterology, Affiliated Hospital of Nantong University, 20 Xisi Road, Nantong, 226001, Jiangsu Province, People's Republic of China.
authors.forename: Weijuan
authors.initials: W
authors.lastname: Zhao
authors.affiliation: Department of Spine Surgery, The Second Affiliated Hospital of Nantong University, Nantong University, Nantong, 226001, Jiangsu Province, People's Republic of China.
authors.forename: Jinlong
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Department of Spine Surgery, The Second Affiliated Hospital of Nantong University, Nantong University, Nantong, 226001, Jiangsu Province, People's Republic of China.
authors.forename: Guofeng
authors.initials: G
authors.lastname: Bao
authors.affiliation: Department of Spine Surgery, The Second Affiliated Hospital of Nantong University, Nantong University, Nantong, 226001, Jiangsu Province, People's Republic of China.
authors.forename: Guanhua
authors.initials: G
authors.lastname: Xu
authors.affiliation: Department of Spine Surgery, The Second Affiliated Hospital of Nantong University, Nantong University, Nantong, 226001, Jiangsu Province, People's Republic of China.
authors.forename: Yuyu
authors.initials: Y
authors.lastname: Sun
authors.affiliation: Department of Spine Surgery, The Second Affiliated Hospital of Nantong University, Nantong University, Nantong, 226001, Jiangsu Province, People's Republic of China.
authors.forename: Jiajia
authors.initials: J
authors.lastname: Chen
authors.affiliation: Department of Spine Surgery, The Second Affiliated Hospital of Nantong University, Nantong University, Nantong, 226001, Jiangsu Province, People's Republic of China. cuizhiming2012@hotmail.com.
authors.forename: Zhiming
authors.initials: Z
authors.lastname: Cui
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D017209:Apoptosis / Q000502:physiology*; D002478:Cells, Cultured; D005786:Gene Expression Regulation; D018976:Insulin-Like Growth Factor Binding Protein 6 / Q000096:biosynthesis*; D008297:Male; D009474:Neurons / Q000378:metabolism* / Q000473:pathology; D051381:Rats; D017207:Rats, Sprague-Dawley; D013119:Spinal Cord Injuries / Q000378:metabolism* / Q000473:pathology; D013904:Thoracic Vertebrae
chemical_list: D018976:Insulin-Like Growth Factor Binding Protein 6
keywords: Apoptosis; Insulin-like growth factor-binding protein-6 (IGFBP6); Neuron; Spinal cord injury (SCI)