title: New subgroup 16SrXI-F phytoplasma strain associated with sugarcane grassy shoot (SCGS) disease in India.
abstract: A novel phytoplasma subgroup strain SC-Phy385 (GenBank accession number HF586648) was found associated with Sugarcane Grassy Shoot (SCGS) disease in India. Sugarcane (Saccharum officinarum L.) leaf samples with 'grassy shoot' symptoms were collected from the western region of Maharashtra state. The analysis based on restriction fragment length polymorphism (RFLP) profiles and 16S rRNA gene sequences indicated that most of the phytoplasma strains belong to the 16SrXI-B group. However, SCGS phytoplasma strain SC-Phy385 showed unique RFLP profiles with a similarity coefficient of 0.87 using online interactive phytoplasma classification tool, iPhyClassifier. The actual and virtual RFLP profiles of SC-Phy385 using amplified products of the 16S rRNA partial gene and its multiple sequence alignment with other RYD group phytoplasma sequences revealed that this strain was different from phytoplasma groups described earlier, representing new subgroup 16SrXI-F. The 16S rRNA gene-based phylogenetic analysis of reported RYD and BGWL group phytoplasmas showed that they are a genetically diverse group of phytoplasmas.
authors.affiliation: Microbial Culture Collection, National Centre for Cell Science, Pashan, Pune 411 021, India.
authors.forename: Amit
authors.initials: A
authors.lastname: Yadav
authors.affiliation: Microbial Culture Collection, National Centre for Cell Science, Pashan, Pune 411 021, India.
authors.forename: Vipool
authors.initials: V
authors.lastname: Thorat
authors.affiliation: Department of Botany, University of Pune, Ganeshkhind, Pune 411 007, India.
authors.forename: Subhash
authors.initials: S
authors.lastname: Deokule
authors.affiliation: Microbial Culture Collection, National Centre for Cell Science, Pashan, Pune 411 021, India.
authors.forename: Yogesh
authors.initials: Y
authors.lastname: Shouche
authors.affiliation: Department of Biotechnology, University of Agriculture Sciences, Gandhi Krishi Vigyan Kendra, Bangalore 560 065, India.
authors.forename: D Theertha
authors.initials: DT
authors.lastname: Prasad
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D007194:India; D010802:Phylogeny; D045582:Phytoplasma / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D010935:Plant Diseases / Q000382:microbiology*; D018515:Plant Leaves / Q000382:microbiology; D012150:Polymorphism, Restriction Fragment Length; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D031786:Saccharum / Q000382:microbiology*; D017422:Sequence Analysis, DNA
chemical_list: D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S