title: FosB regulates expression of miR-22 during PMA induced differentiation of K562 cells to megakaryocytes.
abstract: Expression of many miRNAs is altered in different cancers and these changes are thought to play a key role in formation and progression of cancer. In chronic myelogenous leukemia (CML) a number of miRNAs are known to be down regulated as compared to normal cells. In this report we have investigated the mechanism of this down regulation by using PMA induced differentiation of CML cell line K562 to megakaryocytes as an experimental system. On treatment with PMA, expression of many down regulated miRNAs including miR-22 is induced. PMA also induces expression of several transcription factors, including FosB, EGR1 and EGR2. Our results using a number of approaches, such as promoter reporter assay, FosB knock down and Chip assay, suggest that the expression of miR-22 is regulated transcriptionally by FosB.
authors.affiliation: School of Life Sciences, Jawaharlal Nehru University, New Delhi, 110067, India. Electronic address: hafeezbiotech@gmail.com.
authors.forename: Hafiz M
authors.initials: HM
authors.lastname: Ahmad
authors.affiliation: School of Life Sciences, Jawaharlal Nehru University, New Delhi, 110067, India. Electronic address: pamchui36@gmail.com.
authors.forename: Pamchui
authors.initials: P
authors.lastname: Muiwo
authors.affiliation: School of Life Sciences, Jawaharlal Nehru University, New Delhi, 110067, India. Electronic address: rohinimuthuswami@gmail.com.
authors.forename: Rohini
authors.initials: R
authors.lastname: Muthuswami
authors.affiliation: School of Life Sciences, Jawaharlal Nehru University, New Delhi, 110067, India. Electronic address: alok.bhattacharya@gmail.com.
authors.forename: Alok
authors.initials: A
authors.lastname: Bhattacharya
mesh_terms: D002454:Cell Differentiation / Q000187:drug effects / Q000235:genetics*; D051766:Early Growth Response Protein 1 / Q000096:biosynthesis / Q000235:genetics; D051767:Early Growth Response Protein 2 / Q000096:biosynthesis / Q000235:genetics; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D055785:Gene Knockdown Techniques; D006801:Humans; D020014:K562 Cells; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000235:genetics* / Q000473:pathology; D008533:Megakaryocytes / Q000187:drug effects; D035683:MicroRNAs / Q000096:biosynthesis* / Q000235:genetics; D010767:Phosphorylcholine / Q000031:analogs & derivatives / Q000494:pharmacology; D011109:Polymethacrylic Acids / Q000494:pharmacology; D016760:Proto-Oncogene Proteins c-fos / Q000096:biosynthesis / Q000235:genetics*
chemical_list: C497325:EGR1 protein, human; C497326:EGR2 protein, human; D051766:Early Growth Response Protein 1; D051767:Early Growth Response Protein 2; C064269:FOSB protein, human; C548375:MIRN22 microRNA, human; D035683:MicroRNAs; D011109:Polymethacrylic Acids; D016760:Proto-Oncogene Proteins c-fos; C500651:poly(MPC-co-MA); D010767:Phosphorylcholine
keywords: FosB; K562; PMA; miR-22