title: Diagnostic accuracy of rKLO8 versus rK26 ELISAs for screening of canine visceral leishmaniasis.
abstract: Canine visceral leishmaniasis (CVL) represents an important public health issue. Despite numerous diagnostic tests available, CVL diagnosis still needs to be improved to achieve a more accurate detection rate. Recently, rKLO8, a new antigenic protein of Sudanese Leishmania donovani, was studied for the first time in diagnosis of human visceral leishmaniasis (HVL) and showed good performance. The present study aimed to evaluate serum reactivity to rKL08 and the reference antigen rK26, and to compare both diagnostic proteins with the combined DPP®CVL rapid test and ELISA (EIE-Bio-Manguinhos) confirmatory test, which are both recommended for the diagnosis of CVL in Brazil. Serum samples of dogs were grouped into: (I) DPP®/EIE negative (n=100) and (II) DPP®/EIE positive sera (n=100). Enhanced levels of IgG, mainly IgG2, to both rKLO8 and rK26 were found in group II. Sensitivity was 68% and 77% and specificity was 92% and 91%, for rKLO8 and rK26 antigens, respectively. Moreover, the combination of rKLO8 and rK26 antigens (rKLO8+rK26) exhibited higher sensitivity (85%) and specificity (93%). Thus, our results show that apart from the improved diagnostic power of rKLO8 in HVL, this new antigen is also suitable for the diagnosis of CVL. Further, the combination of rKLO8 and rK26 antigens increases the diagnostic accuracy of CVL.
authors.affiliation: Department of Parasitology, Microbiology and Immunology, Federal University of Juiz de Fora, Juiz de Fora, MG, Brazil.
authors.forename: Lily P
authors.initials: LP
authors.lastname: Martínez Abad
authors.affiliation: Department of Parasitology, Microbiology and Immunology, Federal University of Juiz de Fora, Juiz de Fora, MG, Brazil.
authors.forename: Caroline S
authors.initials: CS
authors.lastname: Almeida
authors.affiliation: Department of Parasitology, Microbiology and Immunology, Federal University of Juiz de Fora, Juiz de Fora, MG, Brazil.
authors.forename: Ana Márcia M
authors.initials: AMM
authors.lastname: Mattos
authors.affiliation: Department of Parasitology, Microbiology and Immunology, Federal University of Juiz de Fora, Juiz de Fora, MG, Brazil.
authors.forename: Ana Carolina P
authors.initials: ACP
authors.lastname: Mendonça
authors.affiliation: Department of Public Health, Faculty of Medicine, Federal University of Juiz de Fora, Juiz de Fora, MG, Brazil.
authors.forename: Márcio J M
authors.initials: MJM
authors.lastname: Alves
authors.affiliation: Zoonosis Control Center, Municipal Health Secretariat, Governador Valadares, MG, Brazil.
authors.forename: Aimara C
authors.initials: AC
authors.lastname: Pinheiro
authors.affiliation: Leishmaniasis Research Laboratory, Oswaldo Cruz Foundation, Rio de Janeiro, Brazil.
authors.forename: Renato
authors.initials: R
authors.lastname: Porrozzi
authors.affiliation: Department of Microbiology, Sudan International University, Khartoum and Laboratory for Biomedical Research, Ahfad University for Women, Omdurman, Sudan.
authors.forename: Elfadil
authors.initials: E
authors.lastname: Abass
authors.affiliation: Department of Medical Microbiology and Hospital Hygiene, Philipps University of Marburg, Germany.
authors.forename: Ulrich
authors.initials: U
authors.lastname: Steinhoff
authors.affiliation: Department of Parasitology, Microbiology and Immunology, Federal University of Juiz de Fora, Juiz de Fora, MG, Brazil. Electronic address: henrique.teixeira@ufjf.edu.br.
authors.forename: Henrique C
authors.initials: HC
authors.lastname: Teixeira
mesh_terms: D000818:Animals; D000953:Antigens, Protozoan / Q000097:blood* / Q000276:immunology; D001938:Brazil; D004283:Dog Diseases / Q000175:diagnosis* / Q000469:parasitology; D004285:Dogs; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000662:veterinary*; D007074:Immunoglobulin G / Q000097:blood / Q000276:immunology; D007893:Leishmania donovani / Q000276:immunology*; D007898:Leishmaniasis, Visceral / Q000175:diagnosis / Q000469:parasitology / Q000662:veterinary*; D012680:Sensitivity and Specificity
chemical_list: D000953:Antigens, Protozoan; D007074:Immunoglobulin G
keywords: Canine visceral leishmaniasis; DPP rapid test; Serodiagnosis; rK26; rKLO8