title: New differentially expressed genes and differential DNA methylation underlying refractory epilepsy.
abstract: Epigenetics underlying refractory epilepsy is poorly understood, especially in patients without distinctive genetic alterations. DNA methylation may affect gene expression in epilepsy without affecting DNA sequences. Herein, we analyzed genome-wide DNA methylation and gene expression in brain tissues of 10 patients with refractory epilepsy using methylated DNA immunoprecipitation linked with sequencing and mRNA Sequencing. Diverse distribution of differentially methylated genes was found in X chromosome, while differentially methylated genes appeared rarely in Y chromosome. 62 differentially expressed genes, such as MMP19, AZGP1, DES, and LGR6 were correlated with refractory epilepsy for the first time. Although general trends of differentially enriched gene ontology terms and Kyoto Encyclopedia of Genes and Genome pathways in this study are consistent with previous researches, differences also exist in many specific gene ontology terms and Kyoto Encyclopedia of Genes and Genome pathways. These findings provide a new genome-wide profiling of DNA methylation and gene expression in brain tissues of patients with refractory epilepsy, which may provide a basis for further study on the etiology and mechanisms of refractory epilepsy.
authors.affiliation: Department of Neurology, The Second Affiliated Hospital of Chongqing Medical University, Yuzhong District, Chongqing, 400010, China.
authors.forename: Xi
authors.initials: X
authors.lastname: Liu
authors.affiliation: Department of Neurology, The Second Affiliated Hospital of Chongqing Medical University, Yuzhong District, Chongqing, 400010, China.
authors.forename: Shu
authors.initials: S
authors.lastname: Ou
authors.affiliation: Department of Neurology, The Second Affiliated Hospital of Chongqing Medical University, Yuzhong District, Chongqing, 400010, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Xu
authors.affiliation: Epilepsy Center of PLA, Department of Neurosurgery, Xinqiao Hospital, The Third Military Medical University, Shapingba District, Chongqing, 400037, China.
authors.forename: Shiyong
authors.initials: S
authors.lastname: Liu
authors.affiliation: Department of Neurology, The Second Affiliated Hospital of Chongqing Medical University, Yuzhong District, Chongqing, 400010, China.
authors.forename: Jinxian
authors.initials: J
authors.lastname: Yuan
authors.affiliation: Department of Neurology, Affiliated Hospital of Zunyi Medical College, Zunyi, 563003, China.
authors.forename: Hao
authors.initials: H
authors.lastname: Huang
authors.affiliation: Department of Neurology, The Second Affiliated Hospital of Chongqing Medical University, Yuzhong District, Chongqing, 400010, China.
authors.forename: Lu
authors.initials: L
authors.lastname: Qin
authors.affiliation: Epilepsy Center of PLA, Department of Neurosurgery, Xinqiao Hospital, The Third Military Medical University, Shapingba District, Chongqing, 400037, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Yang
authors.affiliation: Department of Neurology, The Second Affiliated Hospital of Chongqing Medical University, Yuzhong District, Chongqing, 400010, China.
authors.forename: Lifen
authors.initials: L
authors.lastname: Chen
authors.affiliation: Department of Neurology, The Second Affiliated Hospital of Chongqing Medical University, Yuzhong District, Chongqing, 400010, China.
authors.forename: Xinjie
authors.initials: X
authors.lastname: Tan
authors.affiliation: Department of Neurology, The Second Affiliated Hospital of Chongqing Medical University, Yuzhong District, Chongqing, 400010, China.
authors.forename: Yangmei
authors.initials: Y
authors.lastname: Chen
mesh_terms: D000293:Adolescent; D000328:Adult; D002648:Child; D019175:DNA Methylation; D000069279:Drug Resistant Epilepsy / Q000235:genetics*; D005260:Female; D020869:Gene Expression Profiling; D006801:Humans; D008297:Male; D008875:Middle Aged; D055815:Young Adult
keywords: DNA methylation; epigenetics; gene expression; human; refractory epilepsy