title: Fungal peptides from pneumonitis hypersensitivity etiologic agents are able to induce specific cellular immune response.
abstract: Hypersensitivity pneumonitis (HP) is an immunoallergic disease due to chronic exposure to high quantities of different microorganisms such as Mycobacterium immunogenum (Mi), a mycobacterium, and Lichtheimia corymbifera (Lc), a filamentous fungus. It has recently been demonstrated that the protein DLDH (dihydrolipoyl dehydrogenase), is common to these microorganisms. This study aimed to investigate the immune potential of overlapping peptide pools covering the MiDLDH and LcDLDH.
  
  
  
  A selection of 34 peptides, from the MiDLDH and LcDLDH, able to interact with Major Histocompatibility Complex (MHC) 1 and MHC 2, was obtained using three different epitope prediction websites. By means of ELISPOT assays, we compared the frequency of Interferon gamma (IFNγ) secreting peripheral blood mononuclear cells (PBMC) after stimulation with overlapping peptide pools. Tests were performed using cells from 35 healthy blood donors.
  
  
  
  One peptide pool containing five peptides from MiDLDH and able to interact with MHC 2 induced a marked IFNγ specific immune response (Pool F, p<0.001, Wilcoxon signed-rank test).
  
  
  
  This study demonstrated that peptides from microorganisms involved in HP were able to induce a high IFNγ specific immune response after stimulation of PBMCs from healthy blood donors which could be useful to develop an effective prevention strategy.
authors.affiliation: Chrono-Environnement CNRS 6249 Research Team, Franche-Comté University, Besançon, France; Parasitology-Mycology Department, Besançon University Hospital, Besançon, France. Electronic address: apbellanger@chu-besancon.fr.
authors.forename: Anne-Pauline
authors.initials: AP
authors.lastname: Bellanger
authors.affiliation: Parasitology-Mycology Department, Besançon University Hospital, Besançon, France.
authors.forename: Thibaud
authors.initials: T
authors.lastname: Lignon
authors.affiliation: INSERM Unit 1098, University of Franche-Comté, Besançon, France.
authors.forename: Yann
authors.initials: Y
authors.lastname: Godet
authors.affiliation: Chrono-Environnement CNRS 6249 Research Team, Franche-Comté University, Besançon, France.
authors.forename: Bénédicte
authors.initials: B
authors.lastname: Rognon
authors.affiliation: Chrono-Environnement CNRS 6249 Research Team, Franche-Comté University, Besançon, France; Parasitology-Mycology Department, Besançon University Hospital, Besançon, France.
authors.forename: Gabriel
authors.initials: G
authors.lastname: Reboux
authors.affiliation: Infection Control Department, Besançon University Hospital, Besançon, France.
authors.forename: Houssein
authors.initials: H
authors.lastname: Gbaguidi-Haore
authors.affiliation: INSERM Unit 1098, University of Franche-Comté, Besançon, France.
authors.forename: Christophe
authors.initials: C
authors.lastname: Borg
authors.affiliation: Chrono-Environnement CNRS 6249 Research Team, Franche-Comté University, Besançon, France; Parasitology-Mycology Department, Besançon University Hospital, Besançon, France.
authors.forename: Laurence
authors.initials: L
authors.lastname: Millon
mesh_terms: D000328:Adult; D000368:Aged; D000542:Alveolitis, Extrinsic Allergic / Q000097:blood / Q000276:immunology* / Q000382:microbiology; D000942:Antigens, Bacterial / Q000276:immunology*; D000946:Antigens, Fungal / Q000276:immunology*; D002478:Cells, Cultured; D008058:Dihydrolipoamide Dehydrogenase / Q000276:immunology*; D058501:Enzyme-Linked Immunospot Assay; D018604:Epitope Mapping; D000939:Epitopes; D005260:Female; D064368:Healthy Volunteers; D015395:Histocompatibility Antigens Class I / Q000276:immunology; D000949:Histocompatibility Antigens Class II / Q000276:immunology; D006801:Humans; D007111:Immunity, Cellular; D007371:Interferon-gamma / Q000276:immunology / Q000378:metabolism; D007963:Leukocytes, Mononuclear / Q000276:immunology / Q000382:microbiology; D008297:Male; D008875:Middle Aged; D009090:Mucorales / Q000201:enzymology / Q000276:immunology*; D009161:Mycobacterium / Q000201:enzymology / Q000276:immunology*; D010446:Peptide Fragments / Q000276:immunology*; D018709:Statistics, Nonparametric; D055815:Young Adult
chemical_list: D000942:Antigens, Bacterial; D000946:Antigens, Fungal; D000939:Epitopes; D015395:Histocompatibility Antigens Class I; D000949:Histocompatibility Antigens Class II; C573415:IFNG protein, human; D010446:Peptide Fragments; D007371:Interferon-gamma; D008058:Dihydrolipoamide Dehydrogenase
keywords: ELISPOT assay; Epitopes; Hypersensitivity pneumonitis; IFNγ; T cell