title: Single-step, single-organism bioethanol production and bioconversion of lignocellulose waste materials by phlebioid fungal species.
abstract: Ethanol production from non-pretreated lignocellulose was carried out in a consolidated bioprocess with wood-decay fungi of phlebioid Polyporales. Ethanol production was attempted on glucose, spruce wood sawdust and waste core board. Substantial quantities of ethanol were achieved, and isolate Phlebia radiata 0043 produced 5.9g/L of ethanol reaching the yield of 10.4% ethanol from core board lignocellulose substrate. Acidic initial culture conditions (pH 3) induced ethanol fermentation compared to the more neutral environment. Together with bioethanol, the fungi were able to produce organic acids such as oxalate and fumarate, thus broadening their capacity and applicability as efficient organisms to be utilized for bioconversion of various lignocelluloses. In conclusion, fungi of Phlebia grow on, convert and saccharify solid lignocellulose waste materials without pre-treatments resulting in accumulation of ethanol and organic acids. These findings will aid in applying fungal biotechnology for production of biofuels and biocompounds.
authors.affiliation: Microbiology and Biotechnology, Department of Food and Environmental Sciences, University of Helsinki, FI-00014 Helsinki, Finland.
authors.forename: Hans
authors.initials: H
authors.lastname: Mattila
authors.affiliation: Microbiology and Biotechnology, Department of Food and Environmental Sciences, University of Helsinki, FI-00014 Helsinki, Finland.
authors.forename: Jaana
authors.initials: J
authors.lastname: Kuuskeri
authors.affiliation: Microbiology and Biotechnology, Department of Food and Environmental Sciences, University of Helsinki, FI-00014 Helsinki, Finland. Electronic address: taina.lundell@helsinki.fi.
authors.forename: Taina
authors.initials: T
authors.lastname: Lundell
mesh_terms: D001487:Basidiomycota / Q000378:metabolism*; D056804:Biofuels / Q000032:analysis / Q000382:microbiology; D019149:Bioreactors / Q000382:microbiology*; D000431:Ethanol / Q000032:analysis / Q000737:chemistry / Q000378:metabolism; D005285:Fermentation; D008031:Lignin / Q000032:analysis / Q000737:chemistry / Q000378:metabolism; D012037:Refuse Disposal / Q000379:methods*
chemical_list: D056804:Biofuels; C036909:lignocellulose; D000431:Ethanol; D008031:Lignin
keywords: Bioconversion; Consolidated bioprocessing; Lignocellulose waste; Phlebia radiata; White rot fungi