title: Peptoid Efficacy against Polymicrobial Biofilms Determined by Using Propidium Monoazide-Modified Quantitative PCR.
abstract: Biofilms containing Candida albicans are responsible for a wide variety of clinical infections. The protective effects of the biofilm matrix, the low metabolic activity of microorganisms within a biofilm and their high mutation rate, significantly enhance the resistance of biofilms to conventional antimicrobial treatments. Peptoids are peptide-mimics that share many features of host defence antimicrobial peptides but have increased resistance to proteases and therefore have better stability in vivo. The activity of a library of peptoids was tested against monospecies and polymicrobial bacterial/fungal biofilms. Selected peptoids showed significant bactericidal and fungicidal activity against the polymicrobial biofilms. This coupled with low cytotoxicity suggests that peptoids could offer a new option for the treatment of clinically relevant polymicrobial infections.
authors.affiliation: Centre for Experimental Medicine, The Wellcome-Wolfson Institute for Experimental Medicine, School of Medicine, Dentistry and Biomedical Sciences, Queen's University Belfast, 97 Lisburn Road, Belfast, BT9 7BL, UK.
authors.forename: Yu
authors.initials: Y
authors.lastname: Luo
authors.affiliation: Durham University, Department of Chemistry, Biophysical Sciences Institute, South Road, Durham, DH1 3LE, UK.
authors.forename: Hannah L
authors.initials: HL
authors.lastname: Bolt
authors.affiliation: Durham University, Department of Chemistry, Biophysical Sciences Institute, South Road, Durham, DH1 3LE, UK.
authors.forename: Gabriela A
authors.initials: GA
authors.lastname: Eggimann
authors.affiliation: Centre for Experimental Medicine, The Wellcome-Wolfson Institute for Experimental Medicine, School of Medicine, Dentistry and Biomedical Sciences, Queen's University Belfast, 97 Lisburn Road, Belfast, BT9 7BL, UK.
authors.forename: Danny F
authors.initials: DF
authors.lastname: McAuley
authors.affiliation: Centre for Experimental Medicine, The Wellcome-Wolfson Institute for Experimental Medicine, School of Medicine, Dentistry and Biomedical Sciences, Queen's University Belfast, 97 Lisburn Road, Belfast, BT9 7BL, UK.
authors.forename: Ronan
authors.initials: R
authors.lastname: McMullan
authors.affiliation: Regional Virus Laboratory, Kelvin Building, Royal Victoria Hospital, Belfast Health and Social Care Trust, Grosvenor Road, Belfast, BT12 6BA, UK.
authors.forename: Tanya
authors.initials: T
authors.lastname: Curran
authors.affiliation: School of Pharmacy, Queen's University Belfast, 97 Lisburn Road, Belfast, BT9 7BL, UK.
authors.forename: Mei
authors.initials: M
authors.lastname: Zhou
authors.affiliation: Durham University, School for Biological and Biomedical Sciences, South Road, Durham, DH1 3LE, UK.
authors.forename: Professor Colin A B
authors.initials: PC
authors.lastname: Jahoda
authors.affiliation: Durham University, Department of Chemistry, Biophysical Sciences Institute, South Road, Durham, DH1 3LE, UK.
authors.forename: Steven L
authors.initials: SL
authors.lastname: Cobb
authors.affiliation: Centre for Experimental Medicine, The Wellcome-Wolfson Institute for Experimental Medicine, School of Medicine, Dentistry and Biomedical Sciences, Queen's University Belfast, 97 Lisburn Road, Belfast, BT9 7BL, UK.
authors.forename: Fionnuala T
authors.initials: FT
authors.lastname: Lundy
mesh_terms: D000890:Anti-Infective Agents / Q000737:chemistry / Q000633:toxicity; D001386:Azides / Q000737:chemistry*; D018441:Biofilms / Q000187:drug effects*; D002176:Candida albicans / Q000235:genetics / Q000502:physiology*; D002470:Cell Survival / Q000187:drug effects; D004269:DNA, Bacterial / Q000235:genetics / Q000378:metabolism; D004271:DNA, Fungal / Q000235:genetics / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics / Q000502:physiology; D056945:Hep G2 Cells; D006801:Humans; D034444:Peptoids / Q000737:chemistry / Q000633:toxicity*; D011419:Propidium / Q000031:analogs & derivatives* / Q000737:chemistry; D060888:Real-Time Polymerase Chain Reaction; D013211:Staphylococcus aureus / Q000235:genetics / Q000502:physiology
chemical_list: D000890:Anti-Infective Agents; D001386:Azides; D004269:DNA, Bacterial; D004271:DNA, Fungal; D034444:Peptoids; C533957:propidium monoazide; D011419:Propidium
keywords: antibacterial; antibiotics; antiproliferation; cross kingdom; peptoid; quantification