title: Association of rs1800668 polymorphism in glutathione peroxidase- 1 gene and risk of rheumatoid arthritis in Pakistani population.
abstract: OBJECTIVE
  To investigate the role of glutathione peroxidase 1 (GPX1) C/T polymorphism (rs1800668) in modulating the chances of Rheumatoid arthritis (RA) in Pakistani population.
  
  
  METHODS
  A total of 400 individuals including 200 controls and 200 patients of RA, were genotyped. Detection of rs1800668 polymorphism was carried out using PCR based amplification strategy (allele specific).
  
  
  RESULTS
  The results for Hardy Weinberg Equilibrium (HWE) indicated that the allele frequencies for GPX1 polymorphism were not deviant from HWE in whole population under observation. The statistical analysis indicated that significant association existed between rs1800668 polymorphism and RA (p<0.01). CT genotype increased the risk of RA development by 1.8582 times (OR: 1.8582; 95% CI 1.2154 to 2.8409). CC genotype was found to have protective effect against the disease development (OR: 0.5133; 95% CI 0.3403 to 0.7742) while TT genotype was found to have association with RA development but the risk level was marginal (OR: 1.5319; 95% CI 0.6124 to 3.8322).
  
  
  CONCLUSIONS
  The present finding suggests the importance of GPX1 C/T polymorphism (rs1800668) in development of RA in Pakistani population. The protective role of CC genotype against the development of RA in local population was also observed.
authors.affiliation: Dr. Shazia Irfan, PhD Zoology. Department of Zoology, University of Sargodha, Sargodha, Pakistan.
authors.forename: Shazia
authors.initials: S
authors.lastname: Irfan
authors.affiliation: Asima Rani, M.Phil Zoology. Department of Zoology, University of Sargodha, Sargodha, Pakistan.
authors.forename: Asima
authors.initials: A
authors.lastname: Rani
authors.affiliation: Maryam Sameem, M.phil Zoology. Department of Zoology, University of Sargodha, Sargodha, Pakistan.
authors.forename: Maryam
authors.initials: M
authors.lastname: Sameem
authors.affiliation: Dr. Syed Kashif Nawaz, PhD Zoology. Department of Zoology, University of Sargodha, Sargodha, Pakistan.
authors.forename: Syed Kashif
authors.initials: SK
authors.lastname: Nawaz
authors.affiliation: Dr. Iram Liaqat, PhD Zoology. Department of Zoology, GC University, Lahore, Pakistan.
authors.forename: Iram
authors.initials: I
authors.lastname: Liaqat
authors.affiliation: Dr. Muhammad Arshad, PhD Zoology. University of Education, Lahore, Pakistan.
authors.forename: Muhammad
authors.initials: M
authors.lastname: Arshad
keywords: GPX1 C/T Polymorphism; Rheumatoid Arthritis; rs1800668