title: Phototheranostic nanoplatform based on a single cyanine dye for image-guided combinatorial phototherapy.
abstract: This study represents a novel phototheranostic nanoplatform based on the near-infrared (NIR) heptamethine cyanine dye, IR775, which is capable of concurrent real-time fluorescence imaging and cancer eradication with combinatorial phototherapy. To achieve water solubility and enhance tumor delivery, the hydrophobic IR775 dye was loaded into a biocompatible polymeric nanoparticle with a diameter of ~40nm and slightly negative surface charge (-2.34mV). The nanoparticle-encapsulated hydrophobic IR775 dye (IR775-NP) is characterized by an enhanced fluorescence quantum yield (16%) when compared to the water soluble analogs such as ICG (2.7%) and IR783 (8%). Furthermore, the developed IR-775-NP efficiently generates both heat and reactive oxygen species under NIR light irradiation, eradicating cancer cells in vitro. Finally, animal studies revealed that the IR775-NP accumulates in cancer tumors after systemic administration, efficiently delineates them with NIR fluorescence signal and completely eradicates chemo resistant cancer tissue after a single dose of combinatorial phototherapy.
authors.affiliation: Department of Pharmaceutical Sciences, College of Pharmacy, Oregon State University, Portland, OR, USA.
authors.forename: Tony
authors.initials: T
authors.lastname: Duong
authors.affiliation: Department of Pharmaceutical Sciences, College of Pharmacy, Oregon State University, Portland, OR, USA.
authors.forename: Xiaoning
authors.initials: X
authors.lastname: Li
authors.affiliation: Department of Pharmaceutical Sciences, College of Pharmacy, Oregon State University, Portland, OR, USA.
authors.forename: Bona
authors.initials: B
authors.lastname: Yang
authors.affiliation: Department of Pharmaceutical Sciences, College of Pharmacy, Oregon State University, Portland, OR, USA.
authors.forename: Canan
authors.initials: C
authors.lastname: Schumann
authors.affiliation: Department of Pharmaceutical Sciences, College of Pharmacy, Oregon State University, Portland, OR, USA.
authors.forename: Hassan A
authors.initials: HA
authors.lastname: Albarqi
authors.affiliation: Department of Pharmaceutical Sciences, College of Pharmacy, Oregon State University, Portland, OR, USA. Electronic address: Olena.Taratula@oregonstate.edu.
authors.forename: Olena
authors.initials: O
authors.lastname: Taratula
authors.affiliation: Department of Pharmaceutical Sciences, College of Pharmacy, Oregon State University, Portland, OR, USA. Electronic address: Oleh.Taratula@oregonstate.edu.
authors.forename: Oleh
authors.initials: O
authors.lastname: Taratula
mesh_terms: D000818:Animals; D002232:Carbocyanines / Q000493:pharmacokinetics / Q000627:therapeutic use; D045744:Cell Line, Tumor; D005260:Female; D005456:Fluorescent Dyes / Q000008:administration & dosage / Q000032:analysis / Q000493:pharmacokinetics* / Q000627:therapeutic use*; D006801:Humans; D007211:Indoles / Q000008:administration & dosage / Q000032:analysis / Q000493:pharmacokinetics* / Q000627:therapeutic use*; D051379:Mice; D053758:Nanoparticles / Q000008:administration & dosage / Q000032:analysis; D061848:Optical Imaging / Q000379:methods; D010051:Ovarian Neoplasms / Q000000981:diagnostic imaging / Q000628:therapy*; D010053:Ovary / Q000000981:diagnostic imaging; D010789:Phototherapy / Q000379:methods*; D000068936:Theranostic Nanomedicine / Q000379:methods*
chemical_list: C000601105:2-(2-(2-chloro-3-(2-(1,3-dihydro-1,3,3-trimethyl-2H-indol-2-ylidene)ethylidene)-1-cyclohexen-1-yl)ethenyl)-1,3,3-trimethyl-3H-indolium; D002232:Carbocyanines; D005456:Fluorescent Dyes; D007211:Indoles
keywords: Cancer; Heptamethine cyanine dyes; ICG; IR775; IR783; IR797; Near-infrared imaging; Phototheranostic; Phototherapy