title: Runella palustris sp. nov., isolated from wetland freshwater.
abstract: A novel, Gram-stain-negative, aerobic, non-motile and filamentous bacterial strain, designated HMF3829T, was isolated from wetland freshwater in Gyeong-an wetland, Republic of Korea. A phylogenetic tree based on 16S rRNA gene sequences showed that strain HMF3829T formed a lineage within the genus Runella. Strain HMF3829T was closely related to Runella slithyformis DSM 19594T (95.2 % 16S rRNA gene sequence similarity), Runellalimosa DSM 17973T (94.9 %), Runellazeae NS12T (94.2 %) and Runella defluvii EMB13T (94.0 %). The major fatty acids of strain HMF3829T were iso-C15 : 0, summed feature 3 (comprising C16 : 1ω7c and/or C16 : 1ω6c), C16 : 1ω5c and iso-C17 : 0 3-OH. The predominant isoprenoid quinone was menaquinone 7 (MK-7). The polar lipids of strain HMF3829T consisted of phosphatidylethanolamine, three unidentified aminolipids, one unidentified phospholipid, three unidentified aminophospholipids and 12 unidentified lipids. The DNA G+C content of strain HMF3829T was 46.2 mol%. On the basis of the evidence presented in this study, strain HMF3829T represents a novel species of the genus Runella, for which the name Runella palustris sp. nov. is proposed. The type strain is HMF3829T (=KCTC 42850T=CECT 8978T).
authors.affiliation: Department of Bioscience and Biotechnology, Hankuk University of Foreign Studies, Gyeonggi 17035, Republic of Korea.
authors.forename: Haneul
authors.initials: H
authors.lastname: Kim
authors.affiliation: Department of Bioscience and Biotechnology, Hankuk University of Foreign Studies, Gyeonggi 17035, Republic of Korea.
authors.forename: Heeyoung
authors.initials: H
authors.lastname: Kang
authors.affiliation: Department of Biological Sciences, Inha University, Incheon 22212, Republic of Korea.
authors.forename: Yochan
authors.initials: Y
authors.lastname: Joung
authors.affiliation: Department of Bioscience and Biotechnology, Hankuk University of Foreign Studies, Gyeonggi 17035, Republic of Korea.
authors.forename: Kiseong
authors.initials: K
authors.lastname: Joh
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D041964:Cytophagaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D005618:Fresh Water / Q000382:microbiology*; D010743:Phospholipids / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D056910:Republic of Korea; D017422:Sequence Analysis, DNA; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000737:chemistry; D053833:Wetlands
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; C062629:menaquinone 7