title: Multiplex Immunoassay Profiling.
abstract: Multiplex immunoassays allow for the rapid profiling of biomarker proteins in biological fluids, using less sample and labor than single immunoassays. This chapter details the methods to develop and manufacture multiplex assays for the Luminex®platform. Although assay development is not included here, the same methods can be used to covalently couple antibodies to the Luminex beads and to label antibodies for the screening of sandwich pairs, if needed. The assay optimization, detection of cross-reactivity, and minimizing antibody interactions and matrix interferences will be addressed.
authors.affiliation: Ampersand Biosciences, 3 Main Street, Saranac Lake, NY, USA. lstephen@ampersandbio.com.
authors.forename: Laurie
authors.initials: L
authors.lastname: Stephen
mesh_terms: D015415:Biomarkers; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D040901:Proteomics / Q000379:methods; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D015415:Biomarkers
keywords: Antibody; Biomarker; Disease; Luminex® assay; Multiplex assay