title: Design, synthesis and antifungal activity of novel fenfuram-diarylamine hybrids.
abstract: Ten novel fenfuram-diarylamine hybrids were designed and synthesized. And their antifungal activities against four phytopathogenic fungi have been evaluated in vitro and most of the compounds demonstrated a significant antifungal activities against Rhizoctonia solani and Sclerotinia sclerotiorum. Compound 5e exhibited the most potent antifungal activity against R. solani with an EC50value of 0.037mg/L, far superior to the commercially available fungicide boscalid (EC50=1.71mg/L) and lead fungicide fenfuram (EC50=6.18mg/L). Furthermore, scanning electron microscopy images showed that the mycelia on treated media grew abnormally with tenuous, wizened and overlapping colonies compared to the negative control. Molecular docking studies revealed that compound 5e featured a higher affinity for succinate dehydrogenase (SDH) than fenfuram. Furthermore, it was shown that the 3-chlorophenyl group in compound 5e formed a CH-π interaction with B/Trp-206 and a Cl-π interaction with D/Tyr-128, rendering compound 5e more active than fenfuram against SDH.
authors.affiliation: Key Laboratory of Bio-Resource and Eco-environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu 610064, China.
authors.forename: Hongyu
authors.initials: H
authors.lastname: Wang
authors.affiliation: Key Laboratory of Bio-Resource and Eco-environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu 610064, China.
authors.forename: Xuheng
authors.initials: X
authors.lastname: Gao
authors.affiliation: Key Laboratory of Bio-Resource and Eco-environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu 610064, China.
authors.forename: Xiaoxiao
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Bio-Resource and Eco-environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu 610064, China. Electronic address: jinhong@scu.edu.cn.
authors.forename: Hong
authors.initials: H
authors.lastname: Jin
authors.affiliation: Key Laboratory of Bio-Resource and Eco-environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu 610064, China.
authors.forename: Ke
authors.initials: K
authors.lastname: Tao
authors.affiliation: Key Laboratory of Bio-Resource and Eco-environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu 610064, China. Electronic address: houtplab@scu.edu.cn.
authors.forename: Taiping
authors.initials: T
authors.lastname: Hou
mesh_terms: D000588:Amines / Q000737:chemistry / Q000494:pharmacology*; D000935:Antifungal Agents / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D001203:Ascomycota / Q000187:drug effects*; D004159:Diphenylamine / Q000031:analogs & derivatives* / Q000737:chemistry / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D015195:Drug Design; D005663:Furans / Q000737:chemistry / Q000494:pharmacology*; D008826:Microbial Sensitivity Tests; D015394:Molecular Structure; D012232:Rhizoctonia / Q000187:drug effects*; D013329:Structure-Activity Relationship
chemical_list: D000588:Amines; D000935:Antifungal Agents; D005663:Furans; C000620850:N-(2-((3-chlorophenyl)amino)phenyl)-2-methylfuran-3-carboxamide; D004159:Diphenylamine
keywords: Antifungal activity; Fenfuram-diarylamine hybrid; Molecular docking; Synthesis