title: Association of MBL2 Gene Polymorphism with Dental Caries in Saudi Children.
abstract: The high prevalence of dental caries in children worldwide is a major oral health problem which requires early intervention. Dental caries is mainly caused by the action of acids produced by bacteria in addition to many other factors. Recent genetic studies have reported that a number of genes are associated with the susceptibility to dental caries. The majority of these genes are associated with inflammation, increased susceptibility to infection, and dentine matrix formation. Using the TaqMan assay and direct DNA sequencing, the prevalence of 6 single-nucleotide polymorphisms (SNPs) in MMP9, MBL2, MMP2, and TIMP2 genes was determined in 102 children with caries and in 100 age-matched caries-free controls. Out of the 6 SNPs tested in the 4 selected genes, only rs11003125 in the MBL2 gene was shown to be associated with a high prevalence of caries in our cohort. In addition, haplotype analysis of the 6 SNPs tested revealed that certain haplotypes, namely GT of rs11003125G and rs7501477T and GT of rs7096206G and rs7501477T, were found to be associated with a high prevalence of dental caries in our cohort, while haplotype AG of rs17576A and rs7501477G was found to have a protective effect against dental caries. In conclusion, the data indicate that rs11003125 in the MBL2 gene was shown to be associated with a high prevalence of caries in our cohort, and 2 haplotypes are also involved in the increased susceptibility to dental caries.
authors.affiliation: College of Dentistry, Institute for Research and Medical Consultation (IRMC), University of Dammam, Dammam, Saudi Arabia.
authors.forename: Yousef M
authors.initials: YM
authors.lastname: Alyousef
authors.forename: J Francis
authors.initials: JF
authors.lastname: Borgio
authors.forename: Sayed
authors.initials: S
authors.lastname: AbdulAzeez
authors.forename: Naif
authors.initials: N
authors.lastname: Al-Masoud
authors.forename: Ali A
authors.initials: AA
authors.lastname: Al-Ali
authors.forename: Emad
authors.initials: E
authors.lastname: Al-Shwaimi
authors.forename: Amein K
authors.initials: AK
authors.lastname: Al-Ali
mesh_terms: D000293:Adolescent; D000483:Alleles; D002648:Child; D002675:Child, Preschool; D015331:Cohort Studies; D004245:DMF Index; D003731:Dental Caries / Q000453:epidemiology* / Q000235:genetics*; D003733:Dental Caries Susceptibility / Q000235:genetics*; D005260:Female; D020022:Genetic Predisposition to Disease / Q000235:genetics*; D006239:Haplotypes / Q000235:genetics; D006801:Humans; D008297:Male; D037601:Mannose-Binding Lectin / Q000235:genetics*; D020778:Matrix Metalloproteinase 2 / Q000235:genetics; D020780:Matrix Metalloproteinase 9 / Q000235:genetics; D009909:Oral Health; D020641:Polymorphism, Single Nucleotide; D015995:Prevalence; D012529:Saudi Arabia / Q000453:epidemiology; D019716:Tissue Inhibitor of Metalloproteinase-2 / Q000235:genetics
chemical_list: C445619:MBL2 protein, human; D037601:Mannose-Binding Lectin; C560823:TIMP2 protein, human; D019716:Tissue Inhibitor of Metalloproteinase-2; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9