title: Biodegradation of the ZnO:Eu nanoparticles in the tissues of adult mouse after alimentary application.
abstract: Biodegradable zinc oxide nanoparticles (ZnO NPs) are considered promising materials for future biomedical applications. To fulfil this potential, biodistribution and elimination patterns of ZnO NPs in the living organism need to be resolved. In order to investigate gastrointestinal absorption of ZnO NPs and their intra-organism distribution, water suspension of ZnO or fluorescent ZnO:Eu (Europium-doped zinc oxide) NPs (10mg/ml; 0.3ml/mouse) was alimentary-administered (IG: intra-gastric) to adult mice. Internal organs collected at key time-points after IG were evaluated by AAS for Zn concentration and analysed by cytometric techniques. We found that Zn-based NPs were readily absorbed and distributed (3 h post IG) in the nanoparticle form throughout the organism. Results suggest, that liver and kidneys were key organs responsible for NPs elimination, while accumulation was observed in the spleen and adipose tissues. We also showed that ZnO/ZnO:Eu NPs were able to cross majority of biological barriers in the organism (including blood-brain-barrier).
authors.affiliation: Veterinary Research Centre, Centre for Biomedical Research, Department of Large Animal Diseases with Clinic, Faculty of Veterinary Medicine, Warsaw University of Life Sciences, Warsaw, Poland; Department of Physiological Sciences, Faculty of Veterinary Medicine, Warsaw University of Life Sciences, Warsaw, Poland. Electronic address: pskielbik@op.pl.
authors.forename: Paula
authors.initials: P
authors.lastname: Kielbik
authors.affiliation: Veterinary Research Centre, Centre for Biomedical Research, Department of Large Animal Diseases with Clinic, Faculty of Veterinary Medicine, Warsaw University of Life Sciences, Warsaw, Poland; Department of Physiological Sciences, Faculty of Veterinary Medicine, Warsaw University of Life Sciences, Warsaw, Poland; Institute of Physics, Polish Academy of Sciences, Warsaw, Poland.
authors.forename: Jaroslaw
authors.initials: J
authors.lastname: Kaszewski
authors.affiliation: Institute of Physics, Polish Academy of Sciences, Warsaw, Poland.
authors.forename: Julita
authors.initials: J
authors.lastname: Rosowska
authors.affiliation: Institute of Physics, Polish Academy of Sciences, Warsaw, Poland.
authors.forename: Ewelina
authors.initials: E
authors.lastname: Wolska
authors.affiliation: Institute of Physics, Polish Academy of Sciences, Warsaw, Poland.
authors.forename: Bartłomiej S
authors.initials: BS
authors.lastname: Witkowski
authors.affiliation: Department of Physiological Sciences, Faculty of Veterinary Medicine, Warsaw University of Life Sciences, Warsaw, Poland.
authors.forename: Mikolaj A
authors.initials: MA
authors.lastname: Gralak
authors.affiliation: Veterinary Research Centre, Centre for Biomedical Research, Department of Large Animal Diseases with Clinic, Faculty of Veterinary Medicine, Warsaw University of Life Sciences, Warsaw, Poland.
authors.forename: Zdzisław
authors.initials: Z
authors.lastname: Gajewski
authors.affiliation: Institute of Physics, Polish Academy of Sciences, Warsaw, Poland.
authors.forename: Marek
authors.initials: M
authors.lastname: Godlewski
authors.affiliation: Veterinary Research Centre, Centre for Biomedical Research, Department of Large Animal Diseases with Clinic, Faculty of Veterinary Medicine, Warsaw University of Life Sciences, Warsaw, Poland; Department of Physiological Sciences, Faculty of Veterinary Medicine, Warsaw University of Life Sciences, Warsaw, Poland.
authors.forename: Michal M
authors.initials: MM
authors.lastname: Godlewski
mesh_terms: D000818:Animals; D001812:Blood-Brain Barrier / Q000378:metabolism; D004064:Digestive System / Q000378:metabolism; D005063:Europium / Q000008:administration & dosage / Q000378:metabolism* / Q000493:pharmacokinetics*; D005456:Fluorescent Dyes / Q000008:administration & dosage / Q000378:metabolism / Q000493:pharmacokinetics; D065569:Gastrointestinal Absorption; D007668:Kidney / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D053758:Nanoparticles / Q000008:administration & dosage / Q000032:analysis* / Q000378:metabolism* / Q000648:ultrastructure; D014018:Tissue Distribution; D015034:Zinc Oxide / Q000008:administration & dosage / Q000378:metabolism* / Q000493:pharmacokinetics*
chemical_list: D005456:Fluorescent Dyes; D005063:Europium; D015034:Zinc Oxide
keywords: Biodegradation; Blood–Organ barriers; Nanoparticle circulation; Nanoparticles; ZnO:Eu