title: Methane oxidation in a landfill cover soil reactor: Changing of kinetic parameters and microorganism community structure.
abstract: Changing of CH4oxidation potential and biological characteristics with CH4concentration was studied in a landfill cover soil reactor (LCSR). The maximum rate of CH4oxidation reached 32.40 mol d-1m-2by providing sufficient O2in the LCSR. The kinetic parameters of methane oxidation in landfill cover soil were obtained by fitting substrate diffusion and consumption model based on the concentration profile of CH4and O2. The values of [Formula: see text] (0.93-2.29%) and [Formula: see text] (140-524 nmol kgsoil-DW-1·s-1) increased with CH4concentration (9.25-20.30%), while the values of [Formula: see text] (312.9-2.6%) and [Formula: see text] (1.3 × 10-5to 9.0 × 10-3nmol mL-1h-1) were just the opposite. MiSeq pyrosequencing data revealed that Methylobacter (the relative abundance was decreased with height of LCSR) and Methylococcales_unclassified (the relative abundance was increased expect in H 80) became the key players after incubation with increasing CH4concentration. These findings provide information for assessing CH4oxidation potential and changing of biological characteristics in landfill cover soil.
authors.affiliation: a Faculty of Urban Construction and Environment Engineering, Chongqing University , Chongqing , China.|b School of Chemistry and Chemical Engineering, Chongqing University of Technology , Chongqing , China.
authors.forename: Zhi L
authors.initials: ZL
authors.lastname: Xing
authors.affiliation: a Faculty of Urban Construction and Environment Engineering, Chongqing University , Chongqing , China.|b School of Chemistry and Chemical Engineering, Chongqing University of Technology , Chongqing , China.
authors.forename: Tian T
authors.initials: TT
authors.lastname: Zhao
authors.affiliation: a Faculty of Urban Construction and Environment Engineering, Chongqing University , Chongqing , China.|b School of Chemistry and Chemical Engineering, Chongqing University of Technology , Chongqing , China.
authors.forename: Yan H
authors.initials: YH
authors.lastname: Gao
authors.affiliation: b School of Chemistry and Chemical Engineering, Chongqing University of Technology , Chongqing , China.
authors.forename: Xu
authors.initials: X
authors.lastname: Yang
authors.affiliation: b School of Chemistry and Chemical Engineering, Chongqing University of Technology , Chongqing , China.
authors.forename: Shuai
authors.initials: S
authors.lastname: Liu
authors.affiliation: a Faculty of Urban Construction and Environment Engineering, Chongqing University , Chongqing , China.
authors.forename: Xu Y
authors.initials: XY
authors.lastname: Peng
mesh_terms: D004058:Diffusion; D006801:Humans; D007700:Kinetics; D008697:Methane / Q000737:chemistry*; D008772:Methylococcaceae / Q000502:physiology*; D010084:Oxidation-Reduction; D010100:Oxygen / Q000737:chemistry*; D012037:Refuse Disposal; D012987:Soil / Q000737:chemistry*; D012988:Soil Microbiology; D064091:Waste Disposal Facilities
chemical_list: D012987:Soil; D008697:Methane; D010100:Oxygen
keywords: Biogas; MiSeq sequencing; diffusion; half-saturation constant; landfill: methanotrophs; microbial diversity; model