title: Circulating long-non coding RNAs as biomarkers of left ventricular diastolic function and remodelling in patients with well-controlled type 2 diabetes.
abstract: Contractile dysfunction is underdiagnosed in early stages of diabetic cardiomyopathy. We evaluated the potential of circulating long non-coding RNAs (lncRNAs) as biomarkers of subclinical cardiac abnormalities in type 2 diabetes. Forty-eight men with well-controlled type 2 diabetes and 12 healthy age-matched volunteers were enrolled in the study. Left ventricular (LV) parameters were measured by magnetic resonance imaging. A panel of lncRNAs was quantified in serum by RT-qPCR. No differences in expression levels of lncRNAs were observed between type 2 diabetes patients and healthy volunteers. In patients with type 2 diabetes, long intergenic non-coding RNA predicting cardiac remodeling (LIPCAR) was inversely associated with diastolic function, measured as E/A peak flow (P < 0.050 for all linear models). LIPCAR was positively associated with grade I diastolic dysfunction (P < 0.050 for all logistic models). Myocardial infarction-associated transcript (MIAT) and smooth muscle and endothelial cell-enriched migration/differentiation-associated long noncoding RNA (SENCR) were directly associated with LV mass to LV end-diastolic volume ratio, a marker of cardiac remodelling (P < 0.050 for all linear models). These findings were validated in a sample of 30 patients with well-controlled type 2 diabetes. LncRNAs are independent predictors of diastolic function and remodelling in patients with type 2 diabetes.
authors.affiliation: Cardiovascular Research Center (CSIC-ICCC), Biomedical Research Institute Sant Pau (IIB Sant Pau), Barcelona, Spain.|Institute of Molecular and Translational Therapeutic Strategies (IMTTS), IFB-Tx, Hannover Medical School, Hannover, Germany.
authors.forename: D
authors.initials: D
authors.lastname: de Gonzalo-Calvo
authors.affiliation: Institute of Molecular and Translational Therapeutic Strategies (IMTTS), IFB-Tx, Hannover Medical School, Hannover, Germany.
authors.forename: F
authors.initials: F
authors.lastname: Kenneweg
authors.affiliation: Institute of Molecular and Translational Therapeutic Strategies (IMTTS), IFB-Tx, Hannover Medical School, Hannover, Germany.
authors.forename: C
authors.initials: C
authors.lastname: Bang
authors.affiliation: Department of Medicine, University of Cádiz, Cádiz, Spain.
authors.forename: R
authors.initials: R
authors.lastname: Toro
authors.affiliation: Department of Radiology, Leiden University Medical Center, Leiden, The Netherlands.
authors.forename: R W
authors.initials: RW
authors.lastname: van der Meer
authors.affiliation: Department of Medicine, Kantonsspital Baden AG, Baden, Switzerland.
authors.forename: L J
authors.initials: LJ
authors.lastname: Rijzewijk
authors.affiliation: Department of Internal Medicine, University Medical Center Nijmegen, Nijmegen, The Netherlands.
authors.forename: J W
authors.initials: JW
authors.lastname: Smit
authors.affiliation: Department of Radiology, Leiden University Medical Center, Leiden, The Netherlands.
authors.forename: H J
authors.initials: HJ
authors.lastname: Lamb
authors.affiliation: Cardiovascular Research Center (CSIC-ICCC), Biomedical Research Institute Sant Pau (IIB Sant Pau), Barcelona, Spain.
authors.forename: V
authors.initials: V
authors.lastname: Llorente-Cortes
authors.affiliation: Institute of Molecular and Translational Therapeutic Strategies (IMTTS), IFB-Tx, Hannover Medical School, Hannover, Germany.|Excellence Cluster REBIRTH, Hannover Medical School, Hannover, Germany.|National Heart and Lung Institute, Imperial College, London, UK.
authors.forename: T
authors.initials: T
authors.lastname: Thum
mesh_terms: D015415:Biomarkers / Q000097:blood*; D003924:Diabetes Mellitus, Type 2 / Q000097:blood* / Q000235:genetics / Q000503:physiopathology; D006321:Heart / Q000000981:diagnostic imaging / Q000503:physiopathology; D006801:Humans; D008279:Magnetic Resonance Imaging; D008297:Male; D008875:Middle Aged; D062085:RNA, Long Noncoding / Q000097:blood* / Q000235:genetics; D016277:Ventricular Function, Left; D020257:Ventricular Remodeling
chemical_list: D015415:Biomarkers; D062085:RNA, Long Noncoding