title: Functional Metagenomics to Study Antibiotic Resistance.
abstract: The construction and screening of metagenomic expression libraries has great potential to identify novel genes and their functions. Here, we describe metagenomic library preparation from fecal DNA, screening of libraries for antibiotic resistance genes (ARGs), massively parallel DNA sequencing of the enriched DNA fragments, and a computational pipeline for high-throughput assembly and annotation of functionally selected DNA.
authors.affiliation: Center for Genome Sciences and Systems Biology, Washington University School of Medicine, St. Louis, MO, USA.
authors.forename: Manish
authors.initials: M
authors.lastname: Boolchandani
authors.affiliation: Center for Genome Sciences and Systems Biology, Washington University School of Medicine, St. Louis, MO, USA.|Department of Pathology and Immunology, Washington University School of Medicine, St. Louis, MO, USA.
authors.forename: Sanket
authors.initials: S
authors.lastname: Patel
authors.affiliation: Center for Genome Sciences and Systems Biology, Washington University School of Medicine, St. Louis, MO, USA. dantas@wustl.edu.|Department of Pathology and Immunology, Washington University School of Medicine, St. Louis, MO, USA. dantas@wustl.edu.|Department of Biomedical Engineering, Washington University, St. Louis, MO, USA. dantas@wustl.edu.|Department of Molecular Microbiology, Washington University School of Medicine, St. Louis, MO, USA. dantas@wustl.edu.
authors.forename: Gautam
authors.initials: G
authors.lastname: Dantas
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D019295:Computational Biology; D004247:DNA / Q000378:metabolism; D004352:Drug Resistance, Microbial / Q000187:drug effects / Q000235:genetics*; D056186:Metagenomics / Q000379:methods*; D017422:Sequence Analysis, DNA
chemical_list: D000900:Anti-Bacterial Agents; D004247:DNA
keywords: Antibiotic resistance genes; Functional metagenomics; Functional selections; High-throughput assembly; Massively parallel DNA sequencing; PARFuMS; Profile HMM-based annotation; Resfams; Resistome