title: Lysinibacillus alkalisoli sp. nov., isolated from saline-alkaline soil.
abstract: A Gram-stain-positive, aerobic bacterial strain, designated Y2A20T, with peritrichous flagella was isolated from the top layer saline-alkaline soil, Hangjin Banner, Inner Mongolia, northern China. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain Y2A20T formed a stable clade with 'Lysinibacillus jejuensis' N2-5T. Strain Y2A20T shared highest 16S rRNA gene sequence similarity with 'L. jejuensis' N2-5T (97.4 %), but lower 16S rRNA gene sequence similarities with all other type strains (<97.0 %). The major polar lipids of strain Y2A20T were diphosphatidylglycerol, phosphatidylglycerol, phosphatidyethanolamine, two unknown aminophospholipids and three unknown phospholipids. Menaquinone-7 was the predominant menaquinone, while iso-C15 : 0, iso-C16 : 0 and iso-C17 : 0 were the major cellular fatty acids. Its genomic DNA G+C content was 39.0 mol%. DNA-DNA hybridization revealed that strain Y2A20T showed 26±5 % genomic DNA relatedness with its closest relative, 'L. jejuensis' N2-5T. The results of physiological and biochemical tests allowed the discrimination of strain Y2A20T from its phylogenetic relatives. Lysinibacillus alkalisoli sp. nov. is therefore proposed with Y2A20T (=CGMCC 1.15760T=KCTC 33825T) as the type strain.
authors.affiliation: Institute of Innovation (Baotou), Peking University, Baotou 014030, PR China.|College of Engineering, Peking University, Beijing 100871, PR China.
authors.forename: Ji-Quan
authors.initials: JQ
authors.lastname: Sun
authors.affiliation: Institute of Innovation (Baotou), Peking University, Baotou 014030, PR China.
authors.forename: Lian
authors.initials: L
authors.lastname: Xu
authors.affiliation: College of Engineering, Peking University, Beijing 100871, PR China.|Institute of Innovation (Baotou), Peking University, Baotou 014030, PR China.
authors.forename: Xiao-Lei
authors.initials: XL
authors.lastname: Wu
mesh_terms: D000468:Alkalies; D001406:Bacillaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D015373:Bacterial Typing Techniques; D002681:China; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D009693:Nucleic Acid Hybridization; D010457:Peptidoglycan / Q000737:chemistry; D010743:Phospholipids / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D054712:Salinity; D017422:Sequence Analysis, DNA; D012987:Soil / Q000737:chemistry; D012988:Soil Microbiology; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000737:chemistry
chemical_list: D000468:Alkalies; D004269:DNA, Bacterial; D005227:Fatty Acids; D010457:Peptidoglycan; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D012987:Soil; D024482:Vitamin K 2; C062629:menaquinone 7