title: Protective Effects of StandardizedSiegesbeckia glabrescensExtract and Its Active Compound Kirenol against UVB-Induced Photoaging through Inhibition of MAPK/NF-κB Pathways.
abstract: Anti-photoaging effects of standardizedSiegesbeckia glabrescensextract (SGE) and its major active compound kirenol were investigated using Hs68 human dermal fibroblasts and hairless mice, respectively. UVB-irradiated hairless mice that received oral SGE (600 mg/kg/day) showed reduced wrinkle formation and skinfold thickness compared with the UVB-irradiated control. Furthermore, SGE treatment increased the mRNA levels of collagen synthesis genes (COL1A1, COL3A1, COL4A1, and COL7A1) and activated antioxidant enzyme (catalase), while suppressing matrix metalloproteinase (MMP-2, -3, -9, and -13) expression. In Hs68 fibroblasts, kirenol also significantly suppressed MMP expression while increasing the expression of COL1A1, COL3A1, and COL7A1. Collectively, our data demonstrate that both SGE and kirenol attenuated UVB-induced photoaging in hairless mice and fibroblasts through inhibition of the mitogen-activated protein kinases and nuclear factor kappa B pathways, suggesting that SGE has potential to serve as a natural anti-photoaging nutraceutical.
authors.affiliation: Department of Biotechnology, College of Life Science and Biotechnology, Yonsei University, Seoul 03722, Republic of Korea.
authors.forename: Jongwook
authors.initials: J
authors.lastname: Kim
authors.affiliation: Department of Biotechnology, College of Life Science and Biotechnology, Yonsei University, Seoul 03722, Republic of Korea.
authors.forename: Mi-Bo
authors.initials: MB
authors.lastname: Kim
authors.affiliation: Department of Biotechnology, College of Life Science and Biotechnology, Yonsei University, Seoul 03722, Republic of Korea.
authors.forename: Jun Gon
authors.initials: JG
authors.lastname: Yun
authors.affiliation: Department of Biotechnology, College of Life Science and Biotechnology, Yonsei University, Seoul 03722, Republic of Korea.
authors.forename: Jae Kwan
authors.initials: JK
authors.lastname: Hwang
mesh_terms: D000818:Animals; D019659:Asteraceae / Q000737:chemistry*; D002374:Catalase / Q000235:genetics; D002478:Cells, Cultured; D003094:Collagen / Q000235:genetics; D004224:Diterpenes / Q000494:pharmacology*; D005347:Fibroblasts / Q000187:drug effects*; D006801:Humans; D008297:Male; D045726:Metalloproteases / Q000235:genetics; D051379:Mice; D008812:Mice, Hairless; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism; D016328:NF-kappa B / Q000378:metabolism; D010936:Plant Extracts / Q000494:pharmacology* / Q000592:standards; D012015:Reference Standards; D015398:Signal Transduction / Q000187:drug effects; D015595:Skin Aging / Q000187:drug effects* / Q000528:radiation effects; D012871:Skin Diseases / Q000517:prevention & control*; D014466:Ultraviolet Rays / Q000009:adverse effects
chemical_list: D004224:Diterpenes; D016328:NF-kappa B; D010936:Plant Extracts; C560087:kirenol; D003094:Collagen; D002374:Catalase; D020928:Mitogen-Activated Protein Kinases; D045726:Metalloproteases
keywords: Kirenol; MAPK/NF-κB pathways; Siegesbeckia glabrescens; photoaging