title: Generation of a TLE3 heterozygous knockout human embryonic stem cell line using CRISPR-Cas9.
abstract: Here, we generated a monoallelic mutation in the TLE3 (Transducin Like Enhancer of Split 3) gene using CRISPR-Cas9 editing in the human embryonic stem cell (hESC) line WA01. The heterozygous knockout cell line, TLE3-447-D08-A01, displays partial loss of TLE3 protein expression while maintaining pluripotency, differentiation potential and genomic integrity.
authors.affiliation: Stanley Center for Psychiatric Research, Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA.
authors.forename: Anne M
authors.initials: AM
authors.lastname: Bara
authors.affiliation: Stanley Center for Psychiatric Research, Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA.
authors.forename: Angelica
authors.initials: A
authors.lastname: Messana
authors.affiliation: Stanley Center for Psychiatric Research, Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA.
authors.forename: Amanda
authors.initials: A
authors.lastname: Herring
authors.affiliation: Stanley Center for Psychiatric Research, Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA.
authors.forename: Dane Z
authors.initials: DZ
authors.lastname: Hazelbaker
authors.affiliation: Stanley Center for Psychiatric Research, Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA; Department of Stem Cell and Regenerative Biology, Harvard University, Cambridge, MA 02138, USA.
authors.forename: Kevin
authors.initials: K
authors.lastname: Eggan
authors.affiliation: Stanley Center for Psychiatric Research, Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA; Department of Stem Cell and Regenerative Biology, Harvard University, Cambridge, MA 02138, USA. Electronic address: lbarrett@broadinstitute.org.
authors.forename: Lindy E
authors.initials: LE
authors.lastname: Barrett
mesh_terms: D001483:Base Sequence; D015153:Blotting, Western; D064113:CRISPR-Cas Systems / Q000235:genetics*; D002460:Cell Line; D056970:Co-Repressor Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D058732:Embryoid Bodies / Q000378:metabolism / Q000473:pathology; D006579:Heterozygote; D000066449:Human Embryonic Stem Cells; D006801:Humans; D059785:Karyotype; D060888:Real-Time Polymerase Chain Reaction; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism
chemical_list: D056970:Co-Repressor Proteins; C570667:TLE3 protein, human; D014157:Transcription Factors