title: Mouse Models for Campylobacter jejuni Colonization and Infection.
abstract: Relevant animal models for Campylobacter jejuni infection have been difficult to establish due to C. jejuni's inability to cause disease in many common animal research models. Fortunately, recent work has proven successful in developing several new and relevant mouse models of C. jejuni infection, including the SIGIRR-deficient mouse strain that develops acute enterocolitis in response to C. jejuni. Here we describe how to properly infect mice with C. jejuni, as well as a number of accompanying histological techniques to aid in studying C. jejuni colonization and infection in mice.
authors.affiliation: Division of Gastroenterology, Department of Pediatrics, Child and Family Research Institute, University of British Columbia, Vancouver, BC, V6H 3V4, Canada.
authors.forename: Martin
authors.initials: M
authors.lastname: Stahl
authors.affiliation: Division of Gastroenterology, Department of Pediatrics, Child and Family Research Institute, University of British Columbia, Vancouver, BC, V6H 3V4, Canada.
authors.forename: Franziska A
authors.initials: FA
authors.lastname: Graef
authors.affiliation: Division of Gastroenterology, Department of Pediatrics, Child and Family Research Institute, University of British Columbia, Vancouver, BC, V6H 3V4, Canada. bvallance@cw.bc.ca.
authors.forename: Bruce A
authors.initials: BA
authors.lastname: Vallance
mesh_terms: D000208:Acute Disease; D000367:Age Factors; D000818:Animals; D002169:Campylobacter Infections / Q000235:genetics / Q000382:microbiology / Q000473:pathology*; D016123:Campylobacter jejuni / Q000472:pathogenicity* / Q000502:physiology; D015169:Colony Count, Microbial; D004195:Disease Models, Animal; D004760:Enterocolitis / Q000235:genetics / Q000382:microbiology / Q000473:pathology*; D005260:Female; D005557:Formaldehyde / Q000737:chemistry; D015870:Gene Expression; D007150:Immunohistochemistry / Q000379:methods*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D008856:Microscopy, Fluorescence; D011108:Polymers / Q000737:chemistry; D017472:Receptors, Interleukin-1 / Q000172:deficiency / Q000235:genetics; D012737:Sex Factors; D013194:Staining and Labeling / Q000379:methods; D016707:Tissue Fixation
chemical_list: D011108:Polymers; D017472:Receptors, Interleukin-1; C484529:SIGIRR protein, mouse; D005557:Formaldehyde; C003043:paraform
keywords: Campylobacter jejuni; Histology; Immunofluorescence; Mouse models; Pathology; Staining