title: Low expression of cyclooxygenase-2 in chronic kidney disease in young dogs.
abstract: Chronic kidney disease (CKD) often results in end-stage renal failure in young dogs; however, the pathogenesis of this disease is not established. This study investigated renal expression of cyclooxygenase (COX)-1 and COX-2 proteins in three dogs with chronic kidney disease by immunohistochemistry. Histopathology showed asynchronous differentiation of renal tissues, including immature glomeruli. COX-1 signals were not detected in diseased or normal kidneys. COX-2 signals were low or undetectable in diseased kidneys, while normal kidneys showed clear positive signals in the macula densa (MD). Quantitative scores of COX-2 in diseased kidneys were significantly lower than those in normal kidneys. These findings demonstrate low renal COX-2 expression in CKD in young dogs, but whether this is correlated with disease pathogenesis remains unclear.
authors.affiliation: Laboratory of Veterinary Clinical Pathology, Joint Faculty of Veterinary Medicine, Kagoshima University, Kagoshima 890-0065, Japan. Electronic address: yabu@vet.kagoshima-u.ac.jp.
authors.forename: Akira
authors.initials: A
authors.lastname: Yabuki
authors.affiliation: Laboratory of Veterinary Clinical Pathology, Joint Faculty of Veterinary Medicine, Kagoshima University, Kagoshima 890-0065, Japan.
authors.forename: Akiko
authors.initials: A
authors.lastname: Miyazaki
authors.affiliation: Laboratory of Anatomy, Department of Biomedical Sciences, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo 060-0818, Japan.
authors.forename: Osamu
authors.initials: O
authors.lastname: Ichii
authors.affiliation: Laboratory of Veterinary Clinical Pathology, Joint Faculty of Veterinary Medicine, Kagoshima University, Kagoshima 890-0065, Japan.
authors.forename: Moeko
authors.initials: M
authors.lastname: Kohyama
authors.affiliation: Laboratory of Veterinary Clinical Pathology, Joint Faculty of Veterinary Medicine, Kagoshima University, Kagoshima 890-0065, Japan.
authors.forename: Mariko
authors.initials: M
authors.lastname: Sawa
authors.affiliation: Laboratory of Veterinary Clinical Pathology, Joint Faculty of Veterinary Medicine, Kagoshima University, Kagoshima 890-0065, Japan.
authors.forename: Osamu
authors.initials: O
authors.lastname: Yamato
mesh_terms: D000375:Aging; D000818:Animals; D051545:Cyclooxygenase 1; D051546:Cyclooxygenase 2 / Q000235:genetics / Q000378:metabolism*; D004283:Dog Diseases / Q000378:metabolism*; D004285:Dogs; D015971:Gene Expression Regulation, Enzymologic / Q000502:physiology*; D007150:Immunohistochemistry; D007527:Isoenzymes; D007668:Kidney / Q000378:metabolism; D051436:Renal Insufficiency, Chronic / Q000378:metabolism / Q000662:veterinary*
chemical_list: D007527:Isoenzymes; D051545:Cyclooxygenase 1; D051546:Cyclooxygenase 2
keywords: Chronic kidney disease; Cyclooxygenase-2; Immunohistochemistry; Young dog