title: TP53Gene Status Affects Survival in Advanced Mycosis Fungoides.
abstract: TP53is frequently mutated in different types of neoplasms including leukemia and lymphomas. Mutations ofTP53have also been reported in mycosis fungoides (MF), the most common type of cutaneous lymphoma. However, little is known about the frequency, spectrum of mutations, and their prognostic significance in MF. In this study, we have optimized the protocol for Sanger sequencing ofTP53using DNA extracted from archival paraffin-embedded biopsies. Of 19 samples from patients with stage IIB MF or higher, 31% harbored mutations inTP53. Overall survival of the patients with mutatedTP53was significantly shorter than median survival in the age- and stage-matched patients treated in our Institution. Distribution of mutations was heterogenous inTP53exons; however, C > T transitions were common suggesting the causal role of ultraviolet radiation. We propose thatTP53mutation status would be useful for risk stratification of patients with advanced MF.
authors.affiliation: Department of Pathology, Zealand University Hospital , Roskilde , Denmark.
authors.forename: Gitte
authors.initials: G
authors.lastname: Wooler
authors.affiliation: Department of Pathology, Rigshospitalet , Copenhagen , Denmark.
authors.forename: Linea
authors.initials: L
authors.lastname: Melchior
authors.affiliation: Department of Pathology, Rigshospitalet , Copenhagen , Denmark.
authors.forename: Elisabeth
authors.initials: E
authors.lastname: Ralfkiaer
authors.affiliation: Department of Pathology, Zealand University Hospital , Roskilde , Denmark.
authors.forename: Lise Mette
authors.initials: LM
authors.lastname: Rahbek Gjerdrum
authors.affiliation: Department of Dermatology, Bispebjerg Hospital, Copenhagen, Denmark; Division of Dermatology, Faculty of Medicine and Dentistry, University of Alberta, Edmonton, AB, Canada.
authors.forename: Robert
authors.initials: R
authors.lastname: Gniadecki
keywords: cutaneous lymphoma; mycosis fungoides; p53 mutation; sequencing data analysis; survival rate