title: In-Package Inactivation of Pathogenic and Spoilage Bacteria Associated with Poultry Using Dielectric Barrier Discharge-Cold Plasma Treatments.
abstract: The goal of this study was to test the efficacy of in-package dielectric barrier discharge-cold plasma (DBD-CP) treatment to inactivate poultry-associated spoilage (Pseudomonas fluorescens) and pathogenic (Salmonella enterica Typhimurium, Campylobacter jejuni) bacteria. Liquid cultures of the bacterial isolates were sealed within packages containing ambient air (Trial 1) or modified air (65% O2:30% CO2:5% N2; Trial 2). The packages were subjected to treatment times ranging from 30 to 180 s, and after 24 h incubation at 4 °C, bacterial titers were determined. The DBD-CP system completely inactivated the four isolates tested, although the in-package gas composition and treatment times were isolate-specific. Both C. jejuni isolates were completely inactivated between 30 s (modified air) and 120 s (ambient air), while modified air was required for the complete inactivation of S. typhimurium (90 s) and P. fluorescens (180 s). This DBD-CP system is effective for inactivating major poultry-associated spoilage and pathogenic bacteria in liquid culture, and through this study, system parameters to optimize inactivation were determined. This study demonstrates the potential for DBD-CP treatment to inactivate major bacteria of economic interest to the poultry industry, thus potentially allowing for reduced spoilage (e.g., longer shelf life) and increased safety of poultry products.
authors.affiliation: Egg Safety and Quality Research Unit, US National Poultry Research Center, USDA-ARS, 950 College Station Rd., Athens, GA, 30605, USA. michael.rothrock@ars.usda.gov.
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Rothrock
authors.affiliation: Quality and Safety Assessment Research Unit, US National Poultry Research Center, USDA-ARS, 950 College Station Rd., Athens, GA, 30605, USA.
authors.forename: Hong
authors.initials: H
authors.lastname: Zhuang
authors.affiliation: Quality and Safety Assessment Research Unit, US National Poultry Research Center, USDA-ARS, 950 College Station Rd., Athens, GA, 30605, USA.
authors.forename: Kurt C
authors.initials: KC
authors.lastname: Lawrence
authors.affiliation: Quality and Safety Assessment Research Unit, US National Poultry Research Center, USDA-ARS, 950 College Station Rd., Athens, GA, 30605, USA.
authors.forename: Brian C
authors.initials: BC
authors.lastname: Bowker
authors.affiliation: Quality and Safety Assessment Research Unit, US National Poultry Research Center, USDA-ARS, 950 College Station Rd., Athens, GA, 30605, USA.
authors.forename: Gary R
authors.initials: GR
authors.lastname: Gamble
authors.affiliation: Poultry Microbiology Safety and Processing Research Unit, US National Poultry Research Center, USDA-ARS, 950 College Station Rd., Athens, GA, 30605, USA.
authors.forename: Kelli L
authors.initials: KL
authors.lastname: Hiett
mesh_terms: D000818:Animals; D004203:Disinfection / Q000379:methods*; D005516:Food Microbiology / Q000379:methods*; D006090:Gram-Negative Bacteria / Q000187:drug effects* / Q000502:physiology*; D050296:Microbial Viability / Q000187:drug effects; D058626:Plasma Gases; D011200:Poultry
chemical_list: D058626:Plasma Gases