title: Genomic regions responsible for amenability to Agrobacterium-mediated transformation in barley.
abstract: Different plant cultivars of the same genus and species can exhibit vastly different genetic transformation efficiencies. However, the genetic factors underlying these differences in transformation rate remain largely unknown. In barley, 'Golden Promise' is one of a few cultivars reliable for Agrobacterium-mediated transformation. By contrast, cultivar 'Haruna Nijo' is recalcitrant to genetic transformation. We identified genomic regions of barley important for successful transformation with Agrobacterium, utilizing the 'Haruna Nijo' × 'Golden Promise' F2generation and genotyping by 124 genome-wide SNP markers. We observed significant segregation distortions of these markers from the expected 1:2:1 ratio toward the 'Golden Promise'-type in regions of chromosomes 2H and 3H, indicating that the alleles of 'Golden Promise' in these regions might contribute to transformation efficiency. The same regions, which we termed Transformation Amenability (TFA) regions, were also conserved in transgenic F2plants generated from a 'Morex' × 'Golden Promise' cross. The genomic regions identified herein likely include necessary factors for Agrobacterium-mediated transformation in barley. The potential to introduce these loci into any haplotype of barley opens the door to increasing the efficiency of transformation for target alleles into any haplotype of barley by the TFA-based methods proposed in this report.
authors.affiliation: Institute of Plant Science and Resources, Okayama University, 2-20-1 Chuo, Kurashiki, Okayama 710-0046 Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Hisano
authors.affiliation: Institute of Plant Science and Resources, Okayama University, 2-20-1 Chuo, Kurashiki, Okayama 710-0046 Japan.
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Sato
mesh_terms: D060054:Agrobacterium / Q000502:physiology*; D000483:Alleles; D020090:Chromosome Segregation / Q000235:genetics; D003001:Cloning, Molecular; D003433:Crosses, Genetic; D004843:Epistasis, Genetic; D005787:Gene Frequency / Q000235:genetics; D017343:Genes, Plant; D005816:Genetic Complementation Test; D056426:Genetic Loci; D005819:Genetic Markers; D018745:Genome, Plant; D005838:Genotype; D001467:Hordeum / Q000235:genetics*; D030821:Plants, Genetically Modified; D020641:Polymorphism, Single Nucleotide / Q000235:genetics; D014170:Transformation, Genetic
chemical_list: D005819:Genetic Markers