title: Silver nanoparticle treatment ameliorates biliary atresia syndrome in rhesus rotavirus inoculated mice.
abstract: Biliary atresia (BA) is a neonatal biliary system disease closely associated with viral infection and bile duct inflammation. Silver nanoparticles (AgNps) have previously revealed antiviral and anti-inflammatory properties. In this study, we have investigated the effects of AgNps in the treatment of the Rhesus rotavirus inoculation induced BA in mice. The morphology, liver histopathology, clinical biochemistry examination, and inflammatory cells were analyzed in BA mice. Results indicated that AgNps could significantly increase the survival rate of BA mice, and reduce jaundice and weight lost and the liver enzymes and bilirubin metabolism clinical parameters were close to the normal levels. Diminished numbers of NK cells were observed by flow cytometry analysis and immunohistochemical staining. Furthermore, the viral load was reduced and transcripts for TGF-β mRNA were augmented after AgNps treatment. Collectively, our results suggest that AgNps treatment has beneficial effects on the BA mouse model partially through upregulation of TGF-β.
authors.affiliation: Department of Pediatric Surgery, Guangzhou Women and Children's Medical Center, Guangzhou Medical University, Guangzhou, China.
authors.forename: Ruizhong
authors.initials: R
authors.lastname: Zhang
authors.affiliation: Department of Pediatric Surgery, Guangzhou Women and Children's Medical Center, Guangzhou Medical University, Guangzhou, China.
authors.forename: Zefeng
authors.initials: Z
authors.lastname: Lin
authors.affiliation: Department of Surgery, The University of Hong Kong, Hong Kong, SAR, China.
authors.forename: Vincent C H
authors.initials: VCH
authors.lastname: Lui
authors.affiliation: Department of Surgery, The University of Hong Kong, Hong Kong, SAR, China.
authors.forename: Kenneth K Y
authors.initials: KKY
authors.lastname: Wong
authors.affiliation: Department of Surgery, The University of Hong Kong, Hong Kong, SAR, China.
authors.forename: Paul K H
authors.initials: PKH
authors.lastname: Tam
authors.affiliation: Department of Chemistry, Faculty of Science, University of Hong Kong, Hong Kong, China.
authors.forename: Puiyan
authors.initials: P
authors.lastname: Lee
authors.affiliation: Department of Chemistry, Faculty of Science, University of Hong Kong, Hong Kong, China.
authors.forename: Chun Nam
authors.initials: CN
authors.lastname: Lok
authors.affiliation: Department of Life Sciences, Faculty of Natural Sciences, Imperial College London, UK.
authors.forename: Jonathan R
authors.initials: JR
authors.lastname: Lamb
authors.affiliation: Department of Pediatric Surgery, Guangzhou Women and Children's Medical Center, Guangzhou Medical University, Guangzhou, China; Department of Surgery, The University of Hong Kong, Hong Kong, SAR, China. Electronic address: ychenc@hku.hk.
authors.forename: Yan
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Department of Pediatric Surgery, Guangzhou Women and Children's Medical Center, Guangzhou Medical University, Guangzhou, China. Electronic address: xia-huimin@foxmail.com.
authors.forename: Huimin
authors.initials: H
authors.lastname: Xia
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000627:therapeutic use*; D000998:Antiviral Agents / Q000627:therapeutic use*; D001652:Bile Ducts / Q000187:drug effects / Q000473:pathology; D001656:Biliary Atresia / Q000188:drug therapy* / Q000473:pathology / Q000821:virology; D004195:Disease Models, Animal; D005260:Female; D007565:Jaundice / Q000188:drug therapy / Q000473:pathology / Q000821:virology; D008099:Liver / Q000187:drug effects / Q000473:pathology / Q000821:virology; D053768:Metal Nanoparticles / Q000627:therapeutic use*; D008807:Mice, Inbred BALB C; D012401:Rotavirus / Q000187:drug effects* / Q000302:isolation & purification; D012400:Rotavirus Infections / Q000150:complications / Q000188:drug therapy* / Q000473:pathology; D012834:Silver / Q000627:therapeutic use*
chemical_list: D000893:Anti-Inflammatory Agents; D000998:Antiviral Agents; D012834:Silver
keywords: Biliary atresia; NK cells, TGF-β, hepatic inflammation; Silver nanoparticles