title: Electrochemical Behaviors of Methylene Blue on DNA Modified Electrode and Its Application to the Detection of PCR Product from NOS Sequence.
abstract: An electrochemical DNA biosensor for the detection of NOS gene sequences from genetically modified organisms (GMOs) is presented in this paper. Single-stranded DNA (ssDNA) was covalently attached through the carboxylate ester formed by the 3'- hydroxy end of the DNA with the carboxyl of a mercaptoacetic acid self-assembled monolayer-modified gold electrode using N-hydroxysuccinimide (NHS) and N-(3-dimethylaminopropyl)-N'-ethylcarbodiimide hydrochloride (EDC) as linkers. The electrochemical behavior of methylene blue (MB) on the ssDNA and dsDNA modified gold electrode were carefully studied. Compared with ssDNA/Au electrode, an increase of redox peak current of MB on dsDNA/Au electrode was found, which could be further used for monitoring the recognition of DNA hybridization. Based on this result, the polymerase chain reaction (PCR) product of the common inserts NOS terminator from real GMOs samples was detected successfully.
authors.affiliation: Zibo Entry-Exit Inspection and Quarantine Bureau of P.R. China, Zibo 255031, Shandong, P.R. China.
authors.forename: Ling
authors.initials: L
authors.lastname: Zhu
authors.affiliation: College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, Shandong, P.R. China.
authors.forename: Ruijun
authors.initials: R
authors.lastname: Zhao
authors.affiliation: Zibo Entry-Exit Inspection and Quarantine Bureau of P.R. China, Zibo 255031, Shandong, P.R. China.
authors.forename: Kegang
authors.initials: K
authors.lastname: Wang
authors.affiliation: Zibo Entry-Exit Inspection and Quarantine Bureau of P.R. China, Zibo 255031, Shandong, P.R. China.
authors.forename: Haibo
authors.initials: H
authors.lastname: Xiang
authors.affiliation: College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, Shandong, P.R. China.
authors.forename: Zhimei
authors.initials: Z
authors.lastname: Shang
authors.affiliation: College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, Shandong, P.R. China. sunwei@qust.edu.cn.
authors.forename: Wei
authors.initials: W
authors.lastname: Sun
keywords: Electrochemical DNA biosensor; NOS gene; hybridization; methylene blue; polymerase chain reaction