title: Enhanced legumain-recognition and NIR controlled released of cisplatin-indocyanine nanosphere against gastric carcinoma.
abstract: Cisplatin-therapy has faced limitations in the gastric cancer therapy. To settle the bottleneck, enhanced specificity and controlled-release property are choosen. We synthesize cisplatin and indocyanine green (ICG) loaded PLGA-(DSPE-PEG2000) nanoparticles, which is abbreviated as CINPs. And we conjugate the Gly-Cys-Gly-Ala-Ala-Asn-Leu (GCGAANL) heptapeptide upon the surface of CINPs, the product is abbreviated as ACINPs. ACINPs with nearly 110nm exhibit good monodispersity and size stability. The EE (efficiency of encapsulation) and LE (loading of encapsulation) of cisplatin loaded into ACINPs are optimized as 29.81% and 3.88%. MGC803 cells overexpressing the legumain and MKN28 cells, which negatively express the legumain as well as the normal stomach cells, are selected. In vitro studies have suggested ACINPs, compared with CINPs, could be recognized by MGC803 cells and efficiently killed the cancer cells, while be harmless to MKN28 cells, which indicates the specificity and safety of ACINPs. Under irradiation of 808nm NIR irradiation, ICG loaded in ACINPs could rapidly transform the light to heat up to 60℃. Nanoparticles compared with non-irraditaion group could be quickly disrupted and release the cisplatin which could enhance the controlled-release ability. Hence, the ACINPs exhibit great potential in avoiding the side effects and enhancing the therapy ability of cisplatin.
authors.affiliation: School of Chemistry and Chemical Engineering, Southeast University, Nanjing 210089, PR China.
authors.forename: Tianyi
authors.initials: T
authors.lastname: Shi
authors.affiliation: School of Chemistry and Chemical Engineering, Southeast University, Nanjing 210089, PR China.
authors.forename: Lianshuai
authors.initials: L
authors.lastname: Gu
authors.affiliation: School of Chemistry and Chemical Engineering, Southeast University, Nanjing 210089, PR China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Sun
authors.affiliation: School of Chemistry and Chemical Engineering, Southeast University, Nanjing 210089, PR China.
authors.forename: Senlin
authors.initials: S
authors.lastname: Wang
authors.affiliation: School of Chemistry and Chemical Engineering, Southeast University, Nanjing 210089, PR China.
authors.forename: Chaoqun
authors.initials: C
authors.lastname: You
authors.affiliation: Laboratory of Organic Chemistry, ETH Zurich, 8093 Zurich, Switzerland.
authors.forename: Xiangyang
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Antibody Technique of Ministry of Health, School of Pathology, Nanjing Medical University, Nanjing 210093, PR China.
authors.forename: Jin
authors.initials: J
authors.lastname: Zhu
authors.affiliation: School of Chemistry and Chemical Engineering, Southeast University, Nanjing 210089, PR China. Electronic address: chmsunbw@seu.edu.cn.
authors.forename: Baiwang
authors.initials: B
authors.lastname: Sun
mesh_terms: D000595:Amino Acid Sequence; D000970:Antineoplastic Agents / Q000737:chemistry / Q000494:pharmacology; D001692:Biological Transport; D045744:Cell Line, Tumor; D002945:Cisplatin / Q000737:chemistry* / Q000494:pharmacology; D003546:Cysteine Endopeptidases / Q000378:metabolism*; D003692:Delayed-Action Preparations; D004337:Drug Carriers / Q000737:chemistry / Q000378:metabolism; D006801:Humans; D007208:Indocyanine Green / Q000737:chemistry* / Q000378:metabolism*; D007259:Infrared Rays; D019344:Lactic Acid / Q000737:chemistry; D054118:Nanospheres / Q000737:chemistry*; D009842:Oligopeptides / Q000737:chemistry; D010316:Particle Size; D011092:Polyethylene Glycols / Q000737:chemistry; D011100:Polyglycolic Acid / Q000737:chemistry; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D013274:Stomach Neoplasms / Q000473:pathology*; D000099240:Asparaginyl Endopeptidase
chemical_list: D000970:Antineoplastic Agents; D002945:Cisplatin; D003546:Cysteine Endopeptidases; D003692:Delayed-Action Preparations; D004337:Drug Carriers; D007208:Indocyanine Green; D019344:Lactic Acid; D009842:Oligopeptides; D011092:Polyethylene Glycols; D011100:Polyglycolic Acid; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D000099240:Asparaginyl Endopeptidase; C000595210:polyethylene glycol 2000
keywords: Cisplatin; Controlled-release; Drug delivery; Indocyanine; Legumain-recognition; Lipid-polymer nanoparticle