title: Noncovalent and site-directed spin labeling of duplex RNA.
abstract: An isoindoline-nitroxide derivative of guanine (Ǵ, "G-spin") was shown to bind specifically and effectively to abasic sites in duplex RNAs. Distance measurements on a Ǵ-labeled duplex RNA with PELDOR (DEER) showed a strong orientation dependence. Thus, Ǵ is a readily synthesized, orientation-selective spin label for "mix and measure" PELDOR experiments.
authors.affiliation: Department of Chemistry, Science Institute, University of Iceland, Dunhaga 3, 107 Reykjavik, Iceland. snorrisi@hi.is.
authors.forename: Nilesh R
authors.initials: NR
authors.lastname: Kamble
authors.affiliation: Institute of Physical and Theoretical Chemistry and Center of Biomolecular Magnetic Resonance, Goethe University, Max-von-Laue-Str. 7, 60438 Frankfurt am Main, Hessen, Germany.
authors.forename: Markus
authors.initials: M
authors.lastname: Gränz
authors.affiliation: Institute of Physical and Theoretical Chemistry and Center of Biomolecular Magnetic Resonance, Goethe University, Max-von-Laue-Str. 7, 60438 Frankfurt am Main, Hessen, Germany.
authors.forename: Thomas F
authors.initials: TF
authors.lastname: Prisner
authors.affiliation: Department of Chemistry, Science Institute, University of Iceland, Dunhaga 3, 107 Reykjavik, Iceland. snorrisi@hi.is.
authors.forename: Snorri Th
authors.identifier: http://orcid.org/0000-0003-2492-1456
authors.initials: ST
authors.lastname: Sigurdsson
mesh_terms: D001483:Base Sequence; D004578:Electron Spin Resonance Spectroscopy; D006147:Guanine / Q000737:chemistry; D007211:Indoles / Q000737:chemistry; D015394:Molecular Structure; D009589:Nitrogen Oxides / Q000737:chemistry*; D012313:RNA / Q000737:chemistry*; D013194:Staining and Labeling / Q000379:methods*
chemical_list: D007211:Indoles; D009589:Nitrogen Oxides; D006147:Guanine; D012313:RNA; C057812:indoline; C039900:nitroxyl