title: Sericin ameliorated dysmorphic mitochondria in high-cholesterol diet/streptozotocin rat by antioxidative property.
abstract: Sericin has been implicated in lower cholesterolemic effect due to its properties with several mechanisms. Mitochondria are one of the most important targets to be affected in high blood cholesterol and glucose conditions. The protective role of sericin on mitochondria remains doubtful. To examine this role, electron microscopic, histopathologic, immunohistochemical, and biochemical studies were performed in a high-cholesterol diet/streptozotocin rat model. The results demonstrated that sericin reduced blood cholesterol without hypoglycemic effect. Sericin alleviated dysmorphic mitochondria in heart and liver but not in kidney and also decreased peculiar endoplasmic reticulum in the exocrine pancreas. In addition, sericin decreased hepatic steatosis and preserved zymogen granule referable to the decline of reactive oxygen species production in hepatic mitochondrial extraction and down-regulation of malondialdehyde expression in the liver and exocrine pancreas however irrelevant to lipase activity. This study suggests that sericin has antioxidative property to reduce blood cholesterol by means of diminishing fat deposit in hepatocyte and improves mitochondria and endoplasmic reticulum integrities. [Box: see text].
authors.affiliation: 1 Department of Tropical Pathology, Faculty of Tropical Medicine, Mahidol University, Bangkok 10400, Thailand.
authors.forename: Sumate
authors.initials: S
authors.lastname: Ampawong
authors.affiliation: 2 Department of Microbiology, Faculty of Science, Mahidol University, Bangkok 10400, Thailand.
authors.forename: Duangnate
authors.initials: D
authors.lastname: Isarangkul
authors.affiliation: 3 Bioactive Resources for Innovative Clinical Applications Research Unit and Department of Pharmacy Practice, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok 10330, Thailand.
authors.forename: Pornanong
authors.initials: P
authors.lastname: Aramwit
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000627:therapeutic use*; D001786:Blood Glucose / Q000187:drug effects*; D012831:Bombyx; D002784:Cholesterol / Q000097:blood; D003921:Diabetes Mellitus, Experimental / Q000188:drug therapy*; D059305:Diet, High-Fat; D004721:Endoplasmic Reticulum / Q000378:metabolism; D005234:Fatty Liver / Q000188:drug therapy*; D005260:Female; D006937:Hypercholesterolemia / Q000188:drug therapy*; D007668:Kidney / Q000378:metabolism / Q000648:ultrastructure; D008099:Liver / Q000378:metabolism / Q000648:ultrastructure; D008315:Malondialdehyde / Q000378:metabolism; D008928:Mitochondria / Q000378:metabolism*; D009206:Myocardium / Q000378:metabolism / Q000648:ultrastructure; D018384:Oxidative Stress / Q000187:drug effects; D010179:Pancreas / Q000378:metabolism / Q000648:ultrastructure; D051381:Rats; D017208:Rats, Wistar; D017382:Reactive Oxygen Species / Q000378:metabolism; D047030:Sericins / Q000627:therapeutic use*; D013311:Streptozocin / Q000633:toxicity
chemical_list: D000975:Antioxidants; D001786:Blood Glucose; D017382:Reactive Oxygen Species; D047030:Sericins; D008315:Malondialdehyde; D013311:Streptozocin; D002784:Cholesterol
keywords: Cholesterol; diabetes mellitus; endoplasmic reticulum; mitochondria; oxidative stress; sericin