title: Generation of human induced pluripotent stem cells in defined, feeder-free conditions.
abstract: Herein, we describe a modified protocol for the generation of human induced pluripotent stem cells (hiPS) and expansion under defined, serum free and feeder free conditions. These cells exhibit a high level of plasticity towards various differentiation pathways both in vitro and in vivo. Ultimately, hiPS-derived lines achieved high standards of three dimensional differentiations on biomaterial scaffolds and promoted in vivo regeneration of complex organs, such as Anterior Cruciate Ligament (in swine ACL-rupture models) and other tissues as well.
authors.affiliation: Department of Biomedical Research, Institute of Molecular Biology and Biotechnology, Foundation of Research and Technology-Hellas, University Campus, 45110 Ioannina, Greece.
authors.forename: Athena
authors.initials: A
authors.lastname: Kyrkou
authors.affiliation: Centre for Basic Research, Biomedical Research Foundation of the Academy of Athens, 11527Athens, Greece.
authors.forename: Dimitris
authors.initials: D
authors.lastname: Stellas
authors.affiliation: Laboratory of Biology, Medical School, University of Ioannina, 45110 Ioannina, Greece.
authors.forename: Marika
authors.initials: M
authors.lastname: Syrrou
authors.affiliation: Centre for Basic Research, Biomedical Research Foundation of the Academy of Athens, 11527Athens, Greece.
authors.forename: Apostolos
authors.initials: A
authors.lastname: Klinakis
authors.affiliation: Department of Biomedical Research, Institute of Molecular Biology and Biotechnology, Foundation of Research and Technology-Hellas, University Campus, 45110 Ioannina, Greece; Laboratory of Biological Chemistry, Medical School, University of Ioannina, 45110 Ioannina, Greece; School of Biosciences, College of Life and Environmental Sciences, University of Birmingham, Edgbaston, Birmingham, B15 2TT, UK. Electronic address: kouroupis_d@yahoo.gr.
authors.forename: Theodore
authors.initials: T
authors.lastname: Fotsis
authors.affiliation: Department of Biomedical Research, Institute of Molecular Biology and Biotechnology, Foundation of Research and Technology-Hellas, University Campus, 45110 Ioannina, Greece; School of Biosciences, College of Life and Environmental Sciences, University of Birmingham, Edgbaston, Birmingham, B15 2TT, UK. Electronic address: c.e.murphy@bham.ac.uk.
authors.forename: Carol
authors.initials: C
authors.lastname: Murphy
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods*; D002454:Cell Differentiation; D002460:Cell Line; D065150:Cellular Reprogramming; D055028:Comparative Genomic Hybridization; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism; D020869:Gene Expression Profiling; D005822:Genetic Vectors / Q000235:genetics / Q000378:metabolism; D005838:Genotype; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000166:cytology* / Q000378:metabolism / Q000637:transplantation; D051379:Mice; D016688:Mice, Inbred NOD; D016513:Mice, SCID; D010641:Phenotype; D013724:Teratoma / Q000378:metabolism / Q000473:pathology; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism
chemical_list: D014157:Transcription Factors