title: Effects of artificial tracheal fixation on tracheal epithelial regeneration and prevention of tracheal stenosis.
abstract: CONCLUSIONS
  Tight fixation of the artificial trachea is important for epithelialization and tracheal stenosis.
  
  
  OBJECTIVE
  The authors have developed an artificial trachea and have used it for tracheal reconstruction. Although various studies on tracheal reconstruction have been conducted, no studies have examined the effect of artificial tracheal fixation on tracheal stenosis and regeneration. Therefore, the purpose of the present study was to evaluate the effect of artificial tracheal fixation.
  
  
  METHODS
  Preliminary animal experiment.
  
  
  METHODS
  Artificial tracheae were implanted into rabbits with partial tracheal defects. Tracheal stenosis and regeneration of the tracheal epithelium on the artificial tracheae were evaluated by endoscopic examination, scanning electron microscopic analysis, and histological examination. The artificial tracheae fixed to the tracheal defects were classified into three groups (0-point, 4-point, and 8-point) by the number of fixation points.
  
  
  RESULTS
  At 14 and 28 days post-implantation, the luminal surface of the implantation area was mostly covered with epithelium in all fixation groups. However, a small amount of granulation tissue was observed in the 0-point fixation group at 14 days post-implantation. Moreover, tracheal stenosis did not occur in the 8-point fixation group, but stenosis was detected in the other groups.
authors.affiliation: a Department of Otolaryngology , Fukushima Medical University , Fukushima , Japan.
authors.forename: Yuta
authors.initials: Y
authors.lastname: Nakaegawa
authors.affiliation: a Department of Otolaryngology , Fukushima Medical University , Fukushima , Japan.|b Department of Otolaryngology, Head and Neck Surgery , Kyoto University , Kyoto , Japan.
authors.forename: Ryosuke
authors.initials: R
authors.lastname: Nakamura
authors.affiliation: a Department of Otolaryngology , Fukushima Medical University , Fukushima , Japan.
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Tada
authors.affiliation: a Department of Otolaryngology , Fukushima Medical University , Fukushima , Japan.
authors.forename: Ryo
authors.initials: R
authors.lastname: Suzuki
authors.affiliation: c Division of Animal Sciences , National Institute of Agrobiological Sciences , Ibaraki , Japan.
authors.forename: Toshiaki
authors.initials: T
authors.lastname: Takezawa
authors.affiliation: d Department of Bioartificial Organs , Institute for Frontier Medical Sciences, Kyoto University , Kyoto , Japan.
authors.forename: Tatsuo
authors.initials: T
authors.lastname: Nakamura
authors.affiliation: a Department of Otolaryngology , Fukushima Medical University , Fukushima , Japan.|b Department of Otolaryngology, Head and Neck Surgery , Kyoto University , Kyoto , Japan.
authors.forename: Koichi
authors.initials: K
authors.lastname: Omori
mesh_terms: D000818:Animals; D001187:Artificial Organs; D004724:Endoscopy; D008297:Male; D011817:Rabbits; D012038:Regeneration; D020545:Respiratory Mucosa / Q000502:physiology* / Q000648:ultrastructure; D014132:Trachea; D014135:Tracheal Stenosis / Q000517:prevention & control*
keywords: Tissue regeneration; laryngotracheal stenosis; reconstruction; tracheal stenosis