title: Screening for Cellulase Encoding Clones in Metagenomic Libraries.
abstract: For modern biotechnology there is a steady need to identify novel enzymes. In biotechnological applications, however, enzymes often must function under extreme and nonnatural conditions (i.e., in the presence of solvents, high temperature and/or at extreme pH values). Cellulases have many industrial applications from the generation of bioethanol, a realistic long-term energy source, to the finishing of textiles. These industrial processes require cellulolytic activity under a wide range of pH, temperature, and ionic conditions, and they are usually carried out by mixtures of cellulases. Investigation of the broad diversity of cellulolytic enzymes involved in the natural degradation of cellulose is necessary for optimizing these processes.
authors.affiliation: Microbiology & Biotechnology, Biocenter Klein Flottbek, University of Hamburg, Ohnhorststr. 18, 22609, Hamburg, Germany.
authors.forename: Nele
authors.initials: N
authors.lastname: Ilmberger
authors.affiliation: Microbiology & Biotechnology, Biocenter Klein Flottbek, University of Hamburg, Ohnhorststr. 18, 22609, Hamburg, Germany. wolfgang.streit@uni-hamburg.de.
authors.forename: Wolfgang R
authors.initials: WR
authors.lastname: Streit
mesh_terms: D001709:Biotechnology; D002480:Cellulase / Q000235:genetics* / Q000378:metabolism*; D002482:Cellulose / Q000737:chemistry / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D004789:Enzyme Activation; D015723:Gene Library; D006868:Hydrolysis; D054892:Metagenome; D056186:Metagenomics / Q000379:methods
chemical_list: D002482:Cellulose; D002480:Cellulase
keywords: Bioethanol; Biotechnology; Cellulase; Ionic liquid; Metagenome; Renewable energy