title: A simple and sensitive liquid chromatography-tandem mass spectrometry method for trans-ε-viniferin quantification in mouse plasma and its application to a pharmacokinetic study in mice.
abstract: In this study, a simple and sensitive liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for the quantification of trans-ε-viniferin in small volumes (10μl) of mouse plasma using chlorpropamide as an internal standard was developed and validated. Plasma samples were precipitated with acetonitrile and separated using an Eclipse Plus C18column (100×4.6mm, 1.8-μm) with a mobile phase consisting of 0.1% formic acid in acetonitrile and 0.1% formic acid in water (60:40v/v) at a flow rate of 0.5ml/min. A triple quadrupole mass spectrometer operating in positive ion mode with selected reaction-monitoring mode was used to determine trans-ε-viniferin and chlorpropamide transitions of 455.10→215.05 and 277.00→111.00, respectively. The lower limit of quantification was 5ng/ml with a linear range of 5-2500ng/ml (r≥0.9949). All validation data, including the selectivity, precision, accuracy, recovery, dilution integrity, and stability, conformed to the acceptance requirements. No matrix effects were observed. The developed method was successfully applied to pharmacokinetic studies of trans-ε-viniferin following intravenous (2.5mg/kg), intraperitoneal (2.5, 5 and 10mg/kg), and oral (40mg/kg) administration in mice. This is the first report on the pharmacokinetic properties of trans-ε-viniferin. The results provide a meaningful basis for evaluating the pre-clinical or clinical applications of trans-ε-viniferin.
authors.affiliation: College of Pharmacy and Integrated Research Institute of Pharmaceutical Sciences, The Catholic University of Korea, Bucheon 14662, Republic of Korea.
authors.forename: Jiseon
authors.initials: J
authors.lastname: Kim
authors.affiliation: College of Pharmacy and Integrated Research Institute of Pharmaceutical Sciences, The Catholic University of Korea, Bucheon 14662, Republic of Korea.
authors.forename: Jee Sun
authors.initials: JS
authors.lastname: Min
authors.affiliation: College of Pharmacy and Integrated Research Institute of Pharmaceutical Sciences, The Catholic University of Korea, Bucheon 14662, Republic of Korea.
authors.forename: Doyun
authors.initials: D
authors.lastname: Kim
authors.affiliation: College of Pharmacy and Integrated Research Institute of Pharmaceutical Sciences, The Catholic University of Korea, Bucheon 14662, Republic of Korea.
authors.forename: Yu Fen
authors.initials: YF
authors.lastname: Zheng
authors.affiliation: College of Pharmacy, Dongguk University, Goyang 10326, Republic of Korea.
authors.forename: Karabasappa
authors.initials: K
authors.lastname: Mailar
authors.affiliation: College of Pharmacy, Dongguk University, Goyang 10326, Republic of Korea.
authors.forename: Won Jun
authors.initials: WJ
authors.lastname: Choi
authors.affiliation: College of Pharmacy, Dongguk University, Goyang 10326, Republic of Korea.
authors.forename: Choongho
authors.initials: C
authors.lastname: Lee
authors.affiliation: College of Pharmacy and Integrated Research Institute of Pharmaceutical Sciences, The Catholic University of Korea, Bucheon 14662, Republic of Korea. Electronic address: baesk@catholic.ac.kr.
authors.forename: Soo Kyung
authors.initials: SK
authors.lastname: Bae
mesh_terms: D000818:Animals; D001572:Benzofurans / Q000097:blood* / Q000493:pharmacokinetics*; D002853:Chromatography, Liquid / Q000379:methods / Q000592:standards; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D013267:Stilbenes / Q000097:blood* / Q000493:pharmacokinetics*; D053719:Tandem Mass Spectrometry / Q000379:methods* / Q000592:standards*
chemical_list: D001572:Benzofurans; D013267:Stilbenes; C091891:epsilon-viniferin
keywords: LC–MS/MS; Mouse plasma; Pharmacokinetics; Trans-ε-viniferin