title: Plasma Proteomic Study in Pulmonary Arterial Hypertension Associated with Congenital Heart Diseases.
abstract: Pulmonary arterial hypertension associated with congenital heart disease (CHD-PAH) has serious consequence and plasma protein profiles in CHD-PAH are unknown. We aimed to reveal the differential plasma proteins in 272 CHD patients with or without PAH. Various types of CHD-PAH were studied. Differential plasma proteins were first detected by iTRAQ proteomic technology and those with significant clinical relevance were selected for further ELISA validation in new cohort of patients. Among the 190 differential plasma proteins detected by iTRAQ, carbamoyl-phosphate synthetase I (CPSI, related to urea cycle and endogenous nitric oxide production) and complement factor H-related protein 2 (CFHR2, related to complement system and coagulant mechanism) were selected for further ELISA validation in new cohort of 152 patients. Both CPSI and CFHR2 were down-regulated with decreased plasma levels (p < 0.01). Thus, we for the first time in CHD-PAH patients identified a large number of differential plasma proteins. The decreased CPSI expression in CHD-PAH patients may reveal a mechanism related to endogenous nitric oxide and the decrease of CFHR2 protein may demonstrate the deficiency of the immune system and coagulation mechanism. The findings may open a new direction for translational medicine in CHD-PAH with regard to the diagnosis and progress of the disease.
authors.affiliation: Department of Surgery &Center for Basic Medical Research, TEDA International Cardiovascular Hospital, Chinese Academy of Medical Sciences (CAMS) &Peking Union Medical College (PUMC), Tianjin &The Affiliated Hospital of Hangzhou Normal University &Zhejiang University, Hangzhou, China.
authors.forename: Xi
authors.initials: X
authors.lastname: Zhang
authors.affiliation: TEDA International Cardiovascular Hospital, CAMS &PUMC, Tianjin, China.
authors.forename: Hai-Tao
authors.initials: HT
authors.lastname: Hou
authors.affiliation: TEDA International Cardiovascular Hospital, CAMS &PUMC, Tianjin, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Wang
authors.affiliation: TEDA International Cardiovascular Hospital, CAMS &PUMC, Tianjin, China.
authors.forename: Xiao-Cheng
authors.initials: XC
authors.lastname: Liu
authors.affiliation: TEDA International Cardiovascular Hospital, CAMS &PUMC, Tianjin, China.|The Chinese University of Hong Kong, Shatin, China.
authors.forename: Qin
authors.initials: Q
authors.lastname: Yang
authors.affiliation: Department of Surgery &Center for Basic Medical Research, TEDA International Cardiovascular Hospital, Chinese Academy of Medical Sciences (CAMS) &Peking Union Medical College (PUMC), Tianjin &The Affiliated Hospital of Hangzhou Normal University &Zhejiang University, Hangzhou, China.|Department of Surgery, Oregon Health and Science University, Portland, Oregon, USA.
authors.forename: Guo-Wei
authors.initials: GW
authors.lastname: He
mesh_terms: D001798:Blood Proteins / Q000378:metabolism*; D002648:Child; D002675:Child, Preschool; D005260:Female; D006330:Heart Defects, Congenital / Q000097:blood* / Q000150:complications; D006801:Humans; D006976:Hypertension, Pulmonary / Q000097:blood* / Q000209:etiology; D008297:Male; D040901:Proteomics
chemical_list: D001798:Blood Proteins