title: Spectral pointillism of enhanced Raman scattering for accessing structural and conformational information on single protein.
abstract: In this contribution, we provide new insights on the temporal fluctuations of surface enhanced Raman spectra (SERS) of large single molecules such as proteins. Because they can only fit partly into small active volume, SERS analysis is referred to spectral pointillism where only protein subdomains are shined and the whole protein landscape is built from the dynamics of successive individual spectra. By applying our approach on bovine serum albumin, we show that single protein subdomains are mostly comprised of three distinct amino acids. Surface amino acids such as lysine are preferentially detected in the open form of the protein. The investigation of the tryptophan Fermi doublet in the single protein regime is highly instructive on the protein conformation. We finally demonstrate that spectral pointillism enables to correlate individual amino acids with structural information.
authors.affiliation: Laboratoire Interdisciplinaire Carnot de Bourgogne, UMR 6303 CNRS, Université Bourgogne Franche-Comté, 21000 Dijon, France. aymeric.leray@u-bourgogne.fr eric.finot@u-bourgogne.fr.
authors.forename: Jean-Emmanuel
authors.initials: JE
authors.lastname: Clément
authors.affiliation: Laboratoire Interdisciplinaire Carnot de Bourgogne, UMR 6303 CNRS, Université Bourgogne Franche-Comté, 21000 Dijon, France. aymeric.leray@u-bourgogne.fr eric.finot@u-bourgogne.fr.
authors.forename: Aymeric
authors.initials: A
authors.lastname: Leray
authors.affiliation: Laboratoire Interdisciplinaire Carnot de Bourgogne, UMR 6303 CNRS, Université Bourgogne Franche-Comté, 21000 Dijon, France. aymeric.leray@u-bourgogne.fr eric.finot@u-bourgogne.fr.
authors.forename: Alexandre
authors.initials: A
authors.lastname: Bouhelier
authors.affiliation: Laboratoire Interdisciplinaire Carnot de Bourgogne, UMR 6303 CNRS, Université Bourgogne Franche-Comté, 21000 Dijon, France. aymeric.leray@u-bourgogne.fr eric.finot@u-bourgogne.fr.
authors.forename: Eric
authors.initials: E
authors.lastname: Finot
mesh_terms: D000596:Amino Acids / Q000737:chemistry*; D000818:Animals; D002417:Cattle; D011487:Protein Conformation; D012710:Serum Albumin, Bovine / Q000737:chemistry* / Q000378:metabolism*; D013059:Spectrum Analysis, Raman; D014364:Tryptophan / Q000737:chemistry* / Q000378:metabolism*
chemical_list: D000596:Amino Acids; D012710:Serum Albumin, Bovine; D014364:Tryptophan