title: Increased Levels of miRNA-146a in Serum and Histologic Samples of Patients with Uveal Melanoma.
abstract: Purpose:To analyze MiRs expression in serum of UM patients, respect to healthy donors, and to compare this data with MiRs expressed in formalin-fixed, paraffin-embedded UM samples.Methods:Expression profile of 754 miRNAs was performed in serum of patients with uveal melanoma who underwent primary enucleation. The level of miRNAs increased in serum was individually analyzed on FFPE UM samples and compared to choroidal melanocytes from unaffected eyes.Results:Fourteen patients with uveal melanoma were included in the study. We found 8 serum miRNAs differentially expressed compared to normal controls: 2 upregulated miRNAs (miRNA-146a, miR-523); 6 downregulated miRNAs (miR-19a, miR-30d, miR-127, miR-451, miR-518f, miR-1274B). When data on upregulated miRNAs were singularly validated only a significant overexpression of miRNA-146a was found. A statistically significant upregulation of miRNA-146a was also found on FFPE UM samples, compared to choroidal melanocytes from unaffected eyes.Conclusions:miRNA-146a is increased in serum of patients with UM and in FFPE tumor samples. Further studies will show if it could be considered a potential marker of UM in the blood.
authors.affiliation: Department of Ophthalmology, University of Catania Catania, Italy.
authors.forename: Andrea
authors.initials: A
authors.lastname: Russo
authors.affiliation: Unità di Anatomia Patologica, Department Gian Filippo Ingrassia, University of Catania Catania, Italy.
authors.forename: Rosario
authors.initials: R
authors.lastname: Caltabiano
authors.affiliation: Department of Ophthalmology, University of Catania Catania, Italy.
authors.forename: Antonio
authors.initials: A
authors.lastname: Longo
authors.affiliation: Department of Ophthalmology, University of Catania Catania, Italy.
authors.forename: Teresio
authors.initials: T
authors.lastname: Avitabile
authors.affiliation: Department of Ophthalmology, University of Catania Catania, Italy.
authors.forename: Livio M
authors.initials: LM
authors.lastname: Franco
authors.affiliation: Department of Ophthalmology, University of Catania Catania, Italy.
authors.forename: Vincenza
authors.initials: V
authors.lastname: Bonfiglio
authors.affiliation: Unità di Anatomia Patologica, Department Gian Filippo Ingrassia, University of Catania Catania, Italy.
authors.forename: Lidia
authors.initials: L
authors.lastname: Puzzo
authors.affiliation: Department of Ophthalmology, University of Catania Catania, Italy.
authors.forename: Michele
authors.initials: M
authors.lastname: Reibaldi
keywords: FFPE; enucleation; microRNAs; serum; uveal melanoma