title: Effects of alveolar bone displacement with segmental osteotomy: micro-CT and histomorphometric analysis in rats.
abstract: The purpose of this study was to evaluate the effects of segmental osteotomy on the blood vessels and osteoclasts in rats using micro-computed tomography (micro-CT) and histomorphometric analysis. After segmental osteotomy was performed around the maxillary first molars of 36 male Sprague-Dawley rats (n = 72), the samples were divided into a control group (no displacement), 0.5 D group (0.5 mm buccal displacement) and 1.0 D group (1.0 mm buccal displacement) (n = 24/group). At 1, 2, 4 and 8 weeks after surgery, changes in the blood vessel volume were investigated using micro-CT with perfusion of radiopaque silicone rubber. Tartrate-resistant acid phosphatase (TRAP) staining was used for histomorphometric analysis. Two-way repeated measures analysis of variance (rmANOVA) was performed to compare the volume of blood vessels and number of TRAP-positive osteoclasts among the groups. Regarding blood vessel volume, the displacement groups had no significant effects, while the time points had significant effects (p = 0.014). The blood vessel volume at 1 week was significantly smaller than that at 2, 4, and 8 weeks (p = 0.004, p = 0.026, and p = 0.005, respectively). Regarding TRAP cell count, the displacement groups had no significant effects, while the time points had significant effects (p < 0.001). The number of TRAP-positive osteoclasts at 8 weeks was significantly smaller than that at 1, 2, and 4 weeks (p < 0.001, p < 0.001, and p = 0.002, respectively), and the count at 4 weeks was smaller than that at 1 week (p = 0.011). Therefore, a regional osteoclast-related acceleratory phenomenon was maintained until 4 weeks after surgery.
authors.affiliation: Catholic University of Korea, College of Medicine, Seoul, Korea.
authors.forename: Taegun
authors.initials: T
authors.lastname: Kim
authors.affiliation: Catholic University of Korea, College of Medicine, Uijeongbu St. Mary's Hospital, Department of Dentistry, Uijeongbu-si, Korea.
authors.forename: Won
authors.initials: W
authors.lastname: Lee
authors.affiliation: Ajou University, College of Natural Sciences, Department of Life Sciences, Suwon, Korea.
authors.forename: Sang-Ho
authors.initials: SH
authors.lastname: Baek
authors.affiliation: Catholic University of Korea, College of Medicine, Bucheon St. Mary's Hospital, Department of Dentistry, Seoul, Korea.
authors.forename: Sungwoon
authors.initials: S
authors.lastname: Pyo
authors.affiliation: Catholic University of Korea, College of Medicine, Seoul St. Mary's Hospital, Department of Dentistry, Seoul, Korea.
authors.forename: Yoon-Ah
authors.initials: YA
authors.lastname: Kook
authors.affiliation: Catholic University of Korea, College of Medicine, Seoul St. Mary's Hospital, Department of Dentistry, Seoul, Korea.
authors.forename: Mohamed
authors.initials: M
authors.lastname: Bayome
authors.affiliation: Catholic University of Korea, College of Medicine, Uijeongbu St. Mary's Hospital, Department of Dentistry, Uijeongbu-si, Korea.
authors.forename: Insoo
authors.initials: I
authors.lastname: Kim
mesh_terms: D000539:Alveolar Process / Q000098:blood supply* / Q000000981:diagnostic imaging; D000541:Alveolectomy / Q000379:methods*; D000818:Animals; D002452:Cell Count; D008297:Male; D063166:Maxillary Osteotomy / Q000379:methods*; D008963:Molar; D010010:Osteoclasts; D051381:Rats; D017207:Rats, Sprague-Dawley; D012016:Reference Values; D015203:Reproducibility of Results; D000071681:Tartrate-Resistant Acid Phosphatase; D013997:Time Factors; D055114:X-Ray Microtomography
chemical_list: D000071681:Tartrate-Resistant Acid Phosphatase