title: Multiplex Analysis Using cDNA Transcriptomic Profiling.
abstract: DNA microarrays contain microscopic DNA spots attached to a solid surface. Each spot contains picomolar levels of a specific DNA probe sequence and hybridization to the corresponding gene products can be detected and quantitated through the use of fluorescently labeled target DNA. In this format, DNA microarrays can be used to measure the expression level of thousands of genes in a single experiment. Here, we present a method to detect the mRNA transcriptional changes in neuronal precursor cells following differentiation using high density cDNA microarrays.
authors.affiliation: Bioline Reagents Limited, Unit 16, The Edge Business Centre, Humber Road, London, NW2 6EW, UK. shawkins@bioline.com.
authors.forename: Steve F C
authors.initials: SFC
authors.lastname: Hawkins
authors.affiliation: Laboratory of Neuroproteomics, Department of Biochemistry and Tissue Biology, Institute of Biology, University of Campinas (UNICAMP), Campinas, Brazil.
authors.forename: Paul C
authors.initials: PC
authors.lastname: Guest
mesh_terms: D018076:DNA, Complementary; D020869:Gene Expression Profiling / Q000379:methods*; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D059467:Transcriptome
chemical_list: D018076:DNA, Complementary
keywords: Fluorescent dyes; Multiplex analysis; Quantitation; Taq polymerase; cDNA; mRNA