title: Anti-inflammatory effect of a fatty acid mixture with high ω-9:ω-6 ratio and low ω-6:ω-3 ratio on rats submitted to dental extraction.
abstract: OBJECTIVE
  To evaluate the anti-inflammatory effect of pretreatment for three days with a fatty acid mixture with high ω-9:ω-6 ratio and low ω-6:ω-3 ratio on rats submitted to dental extraction.
  
  
  METHODS
  Thirty-two male Wistar rats (270-310g) were randomly distributed in four groups (n=8/group): the sham control group and the negative control group received saline; the high omega-6/low omega-9 group received isolipid fatty acid with high ω-6:ω-3 ratio and low ω-9:ω-6 ratio; the high omega-3/low omega-6 group received fatty acid with low ω-6:ω-3 ratio and high ω-9:ω-6 ratio. Saline and oils were administered by gavage for 4days before exodontia and 3days after surgery, followed by euthanasia. Masseter edema was evaluated clinically and tissue samples were submitted to osteoclast count (H&E), myeloperoxidase assay, and western blotting (tumor necrosis factor-alpha and interleukin-1beta).
  
  
  RESULTS
  In the high omega-3/low omega-6 group, a significant decrease was observed in masseter edema (p<0.0001), myeloperoxidase (p<0.0001), osteoclasts (p=0.0001) and TNF-α expression (p<0.0001), but not in IL-1β expression.
  
  
  CONCLUSIONS
  The ingestion of fatty acid with high ω-9:ω-6 ratio and low ω-6:ω-3 ratio significantly reduced inflammatory response in rats submitted to dental extraction.
authors.affiliation: Department of Surgery, Faculty of Medicine, Federal University of Ceara, Fortaleza, Ceara, Brazil.
authors.forename: Radamés Bezerra
authors.initials: RB
authors.lastname: Melo
authors.affiliation: Faculty of Dentistry, University Center Christus, Fortaleza, Ceara, Brazil. Electronic address: paulo_goberlanio@yahoo.com.br.
authors.forename: Paulo Goberlânio
authors.initials: PG
authors.lastname: de Barros Silva
authors.affiliation: Department of Morphology, Faculty of Medicine, Federal University of Ceara, Fortaleza, Ceara, Brazil.
authors.forename: Reinaldo Barreto
authors.initials: RB
authors.lastname: Oriá
authors.affiliation: Faculty of Medicine, State University of Ceara, Fortaleza, Ceara, Brazil.
authors.forename: José Ulisses de Souza
authors.initials: JU
authors.lastname: Melo
authors.affiliation: Department of Morphology, Faculty of Medicine, Federal University of Ceara, Fortaleza, Ceara, Brazil.
authors.forename: Conceição
authors.initials: C
authors.lastname: da Silva Martins
authors.affiliation: Department of Surgery, Faculty of Medicine, Federal University of Ceara, Fortaleza, Ceara, Brazil.
authors.forename: Aline Matos
authors.initials: AM
authors.lastname: Cunha
authors.affiliation: Department of Surgery, Faculty of Medicine, Federal University of Ceara, Fortaleza, Ceara, Brazil.
authors.forename: Paulo Roberto Leitão
authors.initials: PR
authors.lastname: Vasconcelos
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D004338:Drug Combinations; D004487:Edema / Q000188:drug therapy; D005227:Fatty Acids / Q000494:pharmacology*; D015525:Fatty Acids, Omega-3 / Q000494:pharmacology*; D043371:Fatty Acids, Omega-6 / Q000494:pharmacology*; D053583:Interleukin-1beta / Q000032:analysis; D008297:Male; D010010:Osteoclasts / Q000187:drug effects; D009195:Peroxidase / Q000032:analysis; D051381:Rats; D017208:Rats, Wistar; D013997:Time Factors; D014081:Tooth Extraction / Q000379:methods*; D016896:Treatment Outcome; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis; D014945:Wound Healing / Q000187:drug effects
chemical_list: D000893:Anti-Inflammatory Agents; D004338:Drug Combinations; D005227:Fatty Acids; D015525:Fatty Acids, Omega-3; D043371:Fatty Acids, Omega-6; D053583:Interleukin-1beta; D014409:Tumor Necrosis Factor-alpha; D009195:Peroxidase
keywords: Anti-inflammatory agents; Antioxidants; Oral; Surgery; Wound healing