title: [Study of tumor necrosis factor-α induced protein 8 like-2 expression in peripheral blood mononuclear cells of patients with acute respiratory distress syndrome correlate with disease severity].
abstract: OBJECTIVE
  To demonstrate the effect of tumor necrosis factor-α induced protein 8 like-2 (TIPE2) expression in patients with acute respiratory distress syndrome (ARDS) and its mechanism.
  
  
  METHODS
  A prospective observation was conducted. Thirty-nine patients with ARDS admitted to department of emergency of PLA General Hospital from July 2013 to July 2015 were enrolled, and 35 healthy persons served as control group. The acute physiology and chronic health evaluation II (APACHE II) score within 24 hours after admission, blood gas analysis, procalcitonin (PCT), and C-reactive protein (CRP) were recorded. The mRNA expressions of TIPE2 in peripheral blood mononuclear cell (PBMC) and myxoma resistance protein 1 (MX1) in plasma were determined by real-time quantitative reverse transcription-polymerase chain reaction (RT-PCR). The correlations were analyzed by Spearman rank correlation analysis.
  
  
  RESULTS
  The mean of APACHE II score in 39 patients with ARDS was 25±3, the mean of PCT was (1.85±0.41) μg/L, and the mean of CRP was (18.0±3.0) mg/L. The TIPE2 mRNA expression in PBMC of ARDS patients was significantly down-regulated as compared with that of healthy control group (2-Κ ΚCt:3.28±0.15 vs. 8.87±0.20, P < 0.001), and the MX-1 mRNA expression in plasma was significantly higher than that of healthy control group (2-Κ ΚCt:39.44±0.46 vs. 20.10±0.32, P < 0.001). It was shown by correlation analysis results that the TIPE2 mRNA expression was negatively correlated with MX1 mRNA expression (r = -0.630, P < 0.001), so as APACHE II score (r = -0.781, P < 0.001), but no correlation was found between TIPE2 mRNA and PCT as well as CRP (r value was 0.143 and 0.330, respectively, both P > 0.05). The MX1 mRNA expression was positively correlated with APACHE II score (r = 0.893, P < 0.001), but no correlation was found between MX1 mRNA and PCT as well as CRP (r value was 0.230 and 0.210, respectively, both P > 0.05).
  
  
  CONCLUSIONS
  TIPE2 expression was decreased in ARDS patients, which negatively correlate with disease severity, and indicate TIPE2 might be involved in the pathogenic process of ARDS.
authors.affiliation: Department of Emergency, Chinese PLA General Hospital, Beijing 100853, China (Huang H, Feng C, Li TS); Department of Critical Care Medicine, Jinan Military General Hospital, Jinan 250031, Shandong, China (Huang H, Tian ZT); Trauma Research Center, First Hospital Affiliated to Chinese PLA General Hospital, Beijing 100048, China (Yao YM). Corresponding author: Li Tanshi, Email: lts301@163.com.
authors.forename: He
authors.initials: H
authors.lastname: Huang
authors.forename: Cong
authors.initials: C
authors.lastname: Feng
authors.forename: Zhaotao
authors.initials: Z
authors.lastname: Tian
authors.forename: Yongming
authors.initials: Y
authors.lastname: Yao
authors.forename: Tanshi
authors.initials: T
authors.lastname: Li
mesh_terms: D018806:APACHE; D002097:C-Reactive Protein; D002116:Calcitonin; D015740:Calcitonin Gene-Related Peptide; D016022:Case-Control Studies; D015536:Down-Regulation; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins / Q000378:metabolism*; D007963:Leukocytes, Mononuclear / Q000378:metabolism; D011446:Prospective Studies; D012333:RNA, Messenger / Q000378:metabolism; D012128:Respiratory Distress Syndrome / Q000378:metabolism*; D014409:Tumor Necrosis Factor-alpha / Q000502:physiology*
chemical_list: D047908:Intracellular Signaling Peptides and Proteins; D012333:RNA, Messenger; C552057:TNFAIP8L2 protein, human; D014409:Tumor Necrosis Factor-alpha; D002116:Calcitonin; D002097:C-Reactive Protein; D015740:Calcitonin Gene-Related Peptide