title: Computational Modeling of the Electrochemical System of Lipase Activity Detection.
abstract: This paper presents computational modeling of response kinetics of bioelectroanalytical system based on solid supported lipase substrate and lipase interaction. The model assumes that lipase substrate is formed by dripping and drying a small amount of the ethanol solution of 9-(5'-ferrocenylpentanoyloxy)nonyl disulfide (FPONDS) and that lipase is capable of cleaving FPONDS ester bonds via hydrolysis mechanism. Two mathematical models have been developed and evaluated trough computational simulation series by comparing them to experimental data. The results of simulation demonstrate that a good fitting might be obtained only taking into account non-linear substrate wash off process.
authors.affiliation: Faculty of Mathematics and Informatics, Vilnius University, Naugarduko 24, LT-03225 Vilnius, Lithuania. mantasp@gmail.com.
authors.forename: Mantas
authors.initials: M
authors.lastname: Puida
authors.affiliation: Faculty of Mathematics and Informatics, Vilnius University, Naugarduko 24, LT-03225 Vilnius, Lithuania.
authors.forename: Feliksas
authors.initials: F
authors.lastname: Ivanauskas
authors.affiliation: Institute of Biochemistry, Mokslininkų 12, LT-08662 Vilnius, Lithuania.
authors.forename: Ilja
authors.initials: I
authors.lastname: Ignatjev
authors.affiliation: Institute of Biochemistry, Mokslininkų 12, LT-08662 Vilnius, Lithuania.
authors.forename: Gintaras
authors.initials: G
authors.lastname: Valinčius
authors.affiliation: Institute of Biochemistry, Mokslininkų 12, LT-08662 Vilnius, Lithuania.
authors.forename: Valdemaras
authors.initials: V
authors.lastname: Razumas
keywords: Amperometric biosensor; ferrocene-based substrate; lipase activity; modeling; simulation