title: Electrochemical Determination of the Antioxidant Potential of Some Less Common Fruit Species.
abstract: Various berries and fruit types of less common fruit species are known to contain antioxidants. Consumption of high amounts of antioxidant flavonoids, which display a variety of biological properties, including antiproliferative and anti-inflammatory activity, may have a positive impact on human health, particularly for the prevention of cancer and other inflammatory diseases. In these studies, based on the hypothesis that the fruit extract with the highest content would possess significantly higher health benefits, flavonoid-rich extracts were obtained from some less common fruit species - Blue Honeysuckles (Lonicera Kamtschatica and Lonicera edulis, Turcz. ex. Freyn), Saskatoon berry (Amelanchier alnifolia Nutt.) and Chinese Hawthorn (Crataegus pinnatifida BUNGE) - grown from germplasm held at the Mendel University of Agriculture and Forestry in Brno, Czech Republic and then characterized in terms of biological value based on the results from a relative antioxidant capacity assessment. The antioxidant content evaluation was based on the total flavonoid amount, determined by liquid chromatography with electrochemical detection (HPLC-ED). A DPPH• test was applied as a reference. The antioxidant content measured in Chinese Hawthorn fruit extract identified it as a potent source of flavonoid antioxidants, with a content 9-fold higher than that seen in Amelanchier fruit. The multifunctional HPLC-ED array method coupled with a DPPH• reference appears to be the optimal analytical progress, accurately reflecting the nutritivetherapeutic properties of a fruit.
authors.affiliation: Department of Breeding and Propagation of Horticultural Plants, Faculty of Agronomy, Mendel University of Agriculture and Forestry, Zemedelska 1, CZ-613 00 Brno, Czech Republic.|Department of Agrochemistry, Soil Science, Microbiology and Plant Nutrition, Faculty of Agronomy, Mendel University of Agriculture and Forestry, Zemedelska 1, CZ-613 00 Brno, Czech Republic.|Department of Chemistry and Biochemistry, Faculty of Agronomy, Mendel University of Agriculture and Forestry, Zemedelska 1, CZ-613 00 Brno, Czech Republic.
authors.forename: Zbynek
authors.initials: Z
authors.lastname: Gazdik
authors.affiliation: Department of Fruit Growing, Faculty of Horticulture, Mendel University of Agriculture and Forestry, Valtická 337, CZ-691 44 Lednice, Czech Republic.
authors.forename: Boris
authors.initials: B
authors.lastname: Krska
authors.affiliation: Department of Chemistry and Biochemistry, Faculty of Agronomy, Mendel University of Agriculture and Forestry, Zemedelska 1, CZ-613 00 Brno, Czech Republic.|Department of Animal Nutrition and Forage Production, Faculty of Agronomy, Mendel University of Agriculture and Forestry, Zemedelska 1, CZ-613 00 Brno, Czech Republic.
authors.forename: Vojtech
authors.initials: V
authors.lastname: Adam
authors.affiliation: Department of Applied Pharmacy, Faculty of Pharmacy, University of Veterinary and Pharmaceutical Sciences, Palackeho 1 - 3, CZ-612 42 Brno, Czech Republic.
authors.forename: Jan
authors.initials: J
authors.lastname: Saloun
authors.affiliation: Institute of Natural and Informatics' Sciences, Faculty of Central European Studies, Constantine the Philosopher University in Nitra, Nabrezie mladeze 91, SK-949 76 Nitra, Slovak Republic.
authors.forename: Tunde
authors.initials: T
authors.lastname: Pokorna
authors.affiliation: Department of Breeding and Propagation of Horticultural Plants, Faculty of Agronomy, Mendel University of Agriculture and Forestry, Zemedelska 1, CZ-613 00 Brno, Czech Republic.
authors.forename: Vojtech
authors.initials: V
authors.lastname: Reznicek
authors.affiliation: Tomas Bata University, T.G. Masaryka 275, CZ-762 72 Zlin, Czech Republic.
authors.forename: Ales
authors.initials: A
authors.lastname: Horna
authors.affiliation: Department of Agrochemistry, Soil Science, Microbiology and Plant Nutrition, Faculty of Agronomy, Mendel University of Agriculture and Forestry, Zemedelska 1, CZ-613 00 Brno, Czech Republic. kizek@sci.muni.cz.
authors.forename: Rene
authors.initials: R
authors.lastname: Kizek
keywords: Antioxidant Capacity; DPPH• test; Flavonoids; Less Common Fruit Species; Liquid Chromatography with Electrochemical Detection