title: [Detection of the mecA gene in coagulase positive Staphylococcus isolated from cats].
abstract: BACKGROUND
  Bacterial resistance is a global concern for public health. Reports of antimicrobial resistance, including that against methicillin, have increased in strains of coagulase positive Staphylococcus (CPS) isolated from pets, however in Chile this information is limited.
  
  
  OBJECTIVE
  To determine the antimicrobial susceptibility profiles and to detect the mecA gene in CPS strains isolated from cats in Chile.
  
  
  METHODS
  134 samples were obtained from healthy cats and cats with skin lesions. These strains were characterized in their coagulase production and identified by BBL Crystal kit. The antimicrobial susceptibility was determined by Kirby Bauer method against 12 antimicrobials, including oxacillin. All strains were subjected to PCR to detect the mecA gene.
  
  
  RESULTS
  72 CPS strains were isolated, including S. aureus and S. intermedius. Antimicrobial resistance against at least one drug was detected in strains from both healthy cats (75%) and from cats with skin lesions (87.5%). The mecA gene was detected in eight methicillin-resistant strains and also in three sensitive strains, being in general multi-resistant.
  
  
  CONCLUSIONS
  These results highlight the role of pets as reservoirs of bacterial resistance, and their potential impact on national public health.
authors.forename: Nicolás
authors.initials: N
authors.lastname: Galarce
authors.forename: Loreto
authors.initials: L
authors.lastname: Muñoz
authors.forename: María Antonieta
authors.initials: MA
authors.lastname: Jara
authors.forename: Paulo
authors.initials: P
authors.lastname: Lubí
authors.forename: Alejandra
authors.initials: A
authors.lastname: Sepúlveda
authors.forename: Sonia
authors.initials: S
authors.lastname: Anticevic
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001426:Bacterial Proteins / Q000235:genetics*; D002415:Cats / Q000382:microbiology*; D002677:Chile; D005798:Genes, Bacterial / Q000187:drug effects / Q000235:genetics; D055624:Methicillin-Resistant Staphylococcus aureus / Q000235:genetics* / Q000302:isolation & purification; D008826:Microbial Sensitivity Tests; D046915:Penicillin-Binding Proteins / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000662:veterinary*
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D046915:Penicillin-Binding Proteins; C487789:mecA protein, Staphylococcus aureus