title: Protective effects of bilobalide against ethanol-induced gastric ulcer in vivo/vitro.
abstract: Bilobalide (BI) has been widely known as a unique constituent extracted from Ginkgo biloba. The aim of the current study was to reveal the potential efficacy as well as the underlying mechanism of the action of BI on ethanol-induced lesion in gastric mucosa in vivo/vitro. Ethanol (0.2ml/kg) was applied to induce gastric ulcer mice model. Our results indicated that treatment with BI markedly decreased the levels of interleukin-6 (IL-6), IL-1β and tumor necrosis factor-α (TNF-α) in vivo. Additionally, BI intervation exhibited elevated myeloperoxidase (MPO) level in stomach, increased superoxide dismutase (SOD) activity and decreased malonaldehyde (MDA) content in serum and stomach when compared with those of the model group. It could be also observed that inhibited MAPK/NF-κB pathway expressions occurred after BI treatment both in vivo and in vitro. Taken together, BI exerted a gastro-protective effect against gastric ulceration, which was presumed to be associated with MAPK/NF-κB pathway.
authors.affiliation: Department of Gastroenterology and Hepatology, Southern Medical University (Guangzhou), Nanjing Clinical School, Jinling Hospital, Nanjing, Jiangsu Province, China.
authors.forename: Shi
authors.initials: S
authors.lastname: Hui
authors.affiliation: Department of Gastroenterology and Hepatology, Southern Medical University (Guangzhou), Nanjing Clinical School, Jinling Hospital, Nanjing, Jiangsu Province, China. Electronic address: wangfy65@nju.edu.cn.
authors.forename: Wang
authors.initials: W
authors.lastname: Fangyu
mesh_terms: D000818:Animals; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D003517:Cyclopentanes / Q000008:administration & dosage / Q000494:pharmacology*; D004847:Epithelial Cells / Q000187:drug effects*; D000431:Ethanol / Q000633:toxicity*; D005260:Female; D005663:Furans / Q000008:administration & dosage / Q000494:pharmacology*; D046934:Ginkgolides / Q000008:administration & dosage / Q000494:pharmacology*; D006801:Humans; D008315:Malondialdehyde / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D013047:Specific Pathogen-Free Organisms; D013270:Stomach / Q000166:cytology / Q000473:pathology; D013276:Stomach Ulcer / Q000139:chemically induced* / Q000517:prevention & control; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D003517:Cyclopentanes; D005663:Furans; D046934:Ginkgolides; D000431:Ethanol; D008315:Malondialdehyde; D013482:Superoxide Dismutase; C073710:bilobalide
keywords: Bilobalide; Ethanol-induced gastric ulcer; MAPK/NF-κB pathway