title: Electric Signals Regulate the Directional Migration of Oligodendrocyte Progenitor Cells (OPCs) via β1 Integrin.
abstract: The guided migration of neural cells is essential for repair in the central nervous system (CNS). Oligodendrocyte progenitor cells (OPCs) will normally migrate towards an injury site to re-sheath demyelinated axons; however the mechanisms underlying this process are not well understood. Endogenous electric fields (EFs) are known to influence cell migration in vivo, and have been utilised in this study to direct the migration of OPCs isolated from neonatal Sprague-Dawley rats. The OPCs were exposed to physiological levels of electrical stimulation, and displayed a marked electrotactic response that was dependent on β1 integrin, one of the key subunits of integrin receptors. We also observed that F-actin, an important component of the cytoskeleton, was re-distributed towards the leading edge of the migrating cells, and that this asymmetric rearrangement was associated with β1 integrin function.
authors.affiliation: Cardiff Institute of Tissue Engineering and Repair, School of Dentistry, College of Biomedical and Life Sciences, Cardiff University, Cardiff CF14 4XY, UK. nsxbz@bristol.ac.uk.|School of Biochemistry, University of Bristol, Bristol BS8 1TD, UK. nsxbz@bristol.ac.uk.
authors.forename: Bangfu
authors.initials: B
authors.lastname: Zhu
authors.affiliation: Cardiff Institute of Tissue Engineering and Repair, School of Dentistry, College of Biomedical and Life Sciences, Cardiff University, Cardiff CF14 4XY, UK. nichollsm2@cardiff.ac.uk.
authors.forename: Matthew
authors.initials: M
authors.lastname: Nicholls
authors.affiliation: Cardiff Institute of Tissue Engineering and Repair, School of Dentistry, College of Biomedical and Life Sciences, Cardiff University, Cardiff CF14 4XY, UK. guY11@cardiff.ac.uk.
authors.forename: Yu
authors.initials: Y
authors.lastname: Gu
authors.affiliation: Cardiff Institute of Tissue Engineering and Repair, School of Dentistry, College of Biomedical and Life Sciences, Cardiff University, Cardiff CF14 4XY, UK. gaofengzhang@gmail.com.
authors.forename: Gaofeng
authors.initials: G
authors.lastname: Zhang
authors.affiliation: Wellcome Trust-Medical Research Council Cambridge Stem Cell Institute, University of Cambridge, Cambridge CB2 1TA, UK. cz213@cam.ac.uk.
authors.forename: Chao
authors.initials: C
authors.lastname: Zhao
authors.affiliation: Wellcome Trust-Medical Research Council Cambridge Stem Cell Institute, University of Cambridge, Cambridge CB2 1TA, UK. rjf1000@cam.ac.uk.
authors.forename: Robin J M
authors.initials: RJ
authors.lastname: Franklin
authors.affiliation: Cardiff Institute of Tissue Engineering and Repair, School of Dentistry, College of Biomedical and Life Sciences, Cardiff University, Cardiff CF14 4XY, UK. songb@cardiff.ac.uk.|Department of Dermatology, No. 1 Hospital of China Medical University, Shenyang 110001, China. songb@cardiff.ac.uk.
authors.forename: Bing
authors.initials: B
authors.lastname: Song
mesh_terms: D000199:Actins / Q000235:genetics / Q000378:metabolism; D000818:Animals; D000831:Animals, Newborn; D002454:Cell Differentiation; D002465:Cell Movement; D003599:Cytoskeleton / Q000378:metabolism* / Q000648:ultrastructure; D004558:Electric Stimulation; D004560:Electricity; D015870:Gene Expression; D019012:Integrin beta1 / Q000235:genetics / Q000378:metabolism*; D058953:Neural Stem Cells / Q000378:metabolism* / Q000648:ultrastructure; D009836:Oligodendroglia / Q000378:metabolism* / Q000648:ultrastructure; D061251:Primary Cell Culture; D021381:Protein Transport; D051381:Rats; D017207:Rats, Sprague-Dawley; D015398:Signal Transduction; D013234:Stem Cells / Q000378:metabolism* / Q000648:ultrastructure
chemical_list: D000199:Actins; D019012:Integrin beta1
keywords: cell migration; electric field; integrin; oligodendrocyte progenitor