title: MnO2nanosheets as an artificial enzyme to mimic oxidase for rapid and sensitive detection of glutathione.
abstract: Nanozymes are increasingly used as components in assays and diagnostics. Here, we describe a rapid and highly sensitive colorimetric assay for the detection and quantification of glutathione (GSH) employing MnO2nanosheets as an artificial oxidase. In the assay pale yellow 3,3´,5,5´-tetramethylbenzidine (TMB) is oxidized to a blue product (oxTMB) under catalyzing of MnO2nanosheets with a significant change in absorption at 650nm. GSH selectively inhibits this reaction with a detection limit of 300nM. The high specificity of inhibition by GSH allows this system to be used to determine the GSH concentrations in human serum samples. The MnO2nanosheet-based assay is simple, rapid, sensitive and selective for the quantification of GSH and surpasses detection methods based on other MnO2nanomaterials.
authors.affiliation: School of Science, State Key Laboratory for Mechanical Behavior of Materials and MOE Key Laboratory for Nonequilibrium Synthesis and Modulation of Condensed Matter, Xi'an Jiaotong University, Xi'an 710049, PR China.
authors.forename: Jing
authors.initials: J
authors.lastname: Liu
authors.affiliation: School of Science, State Key Laboratory for Mechanical Behavior of Materials and MOE Key Laboratory for Nonequilibrium Synthesis and Modulation of Condensed Matter, Xi'an Jiaotong University, Xi'an 710049, PR China. Electronic address: menglingjie@mail.xjtu.edu.cn.
authors.forename: Lingjie
authors.initials: L
authors.lastname: Meng
authors.affiliation: Institut des Sciences et Ingénierie Chimiques, Ecole Polytechnique Fédérale de Lausanne (EPFL), 1015 Lausanne, Switzerland.
authors.forename: Zhaofu
authors.initials: Z
authors.lastname: Fei
authors.affiliation: Institut des Sciences et Ingénierie Chimiques, Ecole Polytechnique Fédérale de Lausanne (EPFL), 1015 Lausanne, Switzerland. Electronic address: paul.dyson@epfl.ch.
authors.forename: Paul J
authors.initials: PJ
authors.lastname: Dyson
authors.affiliation: School of Science, State Key Laboratory for Mechanical Behavior of Materials and MOE Key Laboratory for Nonequilibrium Synthesis and Modulation of Condensed Matter, Xi'an Jiaotong University, Xi'an 710049, PR China.
authors.forename: Xunan
authors.initials: X
authors.lastname: Jing
authors.affiliation: School of Science, State Key Laboratory for Mechanical Behavior of Materials and MOE Key Laboratory for Nonequilibrium Synthesis and Modulation of Condensed Matter, Xi'an Jiaotong University, Xi'an 710049, PR China.
authors.forename: Xing
authors.initials: X
authors.lastname: Liu
mesh_terms: D015374:Biosensing Techniques; D003124:Colorimetry; D005978:Glutathione / Q000737:chemistry / Q000302:isolation & purification*; D006801:Humans; D057230:Limit of Detection; D017895:Manganese Compounds / Q000737:chemistry; D053768:Metal Nanoparticles / Q000737:chemistry*; D010087:Oxides / Q000737:chemistry; D010088:Oxidoreductases / Q000737:chemistry*
chemical_list: D017895:Manganese Compounds; D010087:Oxides; C027424:manganese oxide; D010088:Oxidoreductases; D005978:Glutathione
keywords: Colorimetric assay; Glutathione; MnO(2) nanosheets; Nanozymes; Oxidase