title: Quantitative urinalysis using aggregation-induced emission bioprobes for monitoring chronic kidney disease.
abstract: Early detection and appropriate management of chronic kidney disease can reduce the progression of kidney failure and cardiovascular disease. The urine albumin to creatinine ratio (UACR) test is a standard urine test for identifying individuals at high risk of developing progressive kidney disease. In this study, IDATPE, a novel fluorescent probe with aggregation-induced emission (AIE) features, is successfully developed for creatinine detection and quantitation. An excellent correlation between fluorescent light intensity and creatinine concentration is achieved. In addition, BSPOTPE, a reported excellent AIE bioprobe for human serum albumin (HSA) quantitation, is used together with IDATPE in artificial urine for UACR testing. The mutual interference of HSA and creatinine when the bioprobes are used for quantitation is characterised, with promising results. Further improvements and potential applications in CKD quantitation are highlighted.
authors.affiliation: Centre for NanoScale Science and Technology, School of Computer Science, Engineering and Mathematics, Flinders University, South Australia 5042, Australia. youhong.tang@flinders.edu.au and Department of Medical Biochemistry, School of Medicine, Flinders University, South Australia 5042, Australia.
authors.forename: Tong
authors.initials: T
authors.lastname: Chen
authors.affiliation: Department of Chemistry, The Hong Kong University of Science and Technology, Hong Kong, China. tangbenz@ust.hk.
authors.forename: Ni
authors.initials: N
authors.lastname: Xie
authors.affiliation: Department of Chemistry, The Hong Kong University of Science and Technology, Hong Kong, China. tangbenz@ust.hk.
authors.forename: Lucia
authors.initials: L
authors.lastname: Viglianti
authors.affiliation: Centre for NanoScale Science and Technology, School of Computer Science, Engineering and Mathematics, Flinders University, South Australia 5042, Australia. youhong.tang@flinders.edu.au and Department of Gastroenterology and Hepatology, Flinders Centre for Innovation in Cancer, Flinders University, South Australia 5042, Australia.
authors.forename: Yabin
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: The First Affiliated Hospital of Shenzhen University, Shenzhen Key Laboratory of Neurosurgery, Shenzhen 518035, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Tan
authors.affiliation: Department of Chemistry, The Hong Kong University of Science and Technology, Hong Kong, China. tangbenz@ust.hk.
authors.forename: Ben Zhong
authors.initials: BZ
authors.lastname: Tang
authors.affiliation: Centre for NanoScale Science and Technology, School of Computer Science, Engineering and Mathematics, Flinders University, South Australia 5042, Australia. youhong.tang@flinders.edu.au.
authors.forename: Youhong
authors.identifier: 0000-0003-2718-544X
authors.initials: Y
authors.lastname: Tang
mesh_terms: D003404:Creatinine / Q000032:analysis; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000032:analysis* / Q000138:chemical synthesis / Q000737:chemistry; D006801:Humans; D015394:Molecular Structure; D051436:Renal Insufficiency, Chronic / Q000000981:diagnostic imaging*; D000075462:Serum Albumin, Human / Q000032:analysis; D016482:Urinalysis / Q000379:methods*
chemical_list: D005456:Fluorescent Dyes; D003404:Creatinine; D000075462:Serum Albumin, Human