title: Recombinant Hemagglutinin of Avian Influenza Virus H5 Expressed in the Chloroplast of Chlamydomonas reinhardtii and Evaluation of Its Immunogenicity in Chickens.
abstract: Globally, avian influenza (AI) is a serious problem in poultry farming. Despite vaccination, the prevalence of AI in México highlights the need for new approaches to control AI and to reduce the economic losses associated with its occurrence in susceptible birds. Recombinant proteins from avian influenza virus (AIV) have been expressed in different organisms, such as plants. The present study investigated the feasibility of designing and expressing the HA protein of AIV in the transplastomic microalga Chlamydomonas reinhardtii as a novel approach for AIV control and taking advantage of culture conditions, its reproductive range, and safe use in consideration of the generally regarded as safe food ingredient regulatory classification. The results showed that the HA protein of AIV in C. reinhardtii presents antigenic activity by western blot test and through its application in chickens, demonstrating its feasibility as a recombinant antigen against AIV.
authors.affiliation: A Viren SA de CV, Presidente Benito Juárez 110B, José María Arteaga, Querétaro, Querétaro. 76135, México.
authors.forename: Inkar
authors.initials: I
authors.lastname: Castellanos-Huerta
authors.affiliation: A Viren SA de CV, Presidente Benito Juárez 110B, José María Arteaga, Querétaro, Querétaro. 76135, México.
authors.forename: Bernardo
authors.initials: B
authors.lastname: Bañuelos-Hernández
authors.affiliation: B Department of Poultry Science, University of Arkansas, Fayetteville, AR 72701.
authors.forename: Guillermo
authors.initials: G
authors.lastname: Téllez
authors.affiliation: C Laboratorio de Biofarmacéuticos Recombinantes, Facultad de Ciencias Químicas, Universidad Autónoma de San Luis Potosí, Av. Dr. Manuel Nava 6, 78210, San Luis Potosí, SLP, México.
authors.forename: Sergio
authors.initials: S
authors.lastname: Rosales-Mendoza
authors.affiliation: D Laboratorio Nacional de Genómica para la Biodiversidad, Centro de Investigación y de Estudios Avanzados del IPN, Apartado Postal 629, CP 36500 Irapuato, Guanajuato, México.
authors.forename: Luis G
authors.initials: LG
authors.lastname: Brieba
authors.affiliation: A Viren SA de CV, Presidente Benito Juárez 110B, José María Arteaga, Querétaro, Querétaro. 76135, México.
authors.forename: Elizabeth
authors.initials: E
authors.lastname: Esquivel-Ramos
authors.affiliation: A Viren SA de CV, Presidente Benito Juárez 110B, José María Arteaga, Querétaro, Querétaro. 76135, México.
authors.forename: Josué I
authors.initials: JI
authors.lastname: Beltrán-López
authors.affiliation: E Universidad de Guadalajara, Centro Universitario de Ciencias Exactas e Ingenierías, Departamento de Química, Blvd. Marcelino García Barragán #1421, CP 44430, Guadalajara, Jalisco, México.
authors.forename: Gilberto
authors.initials: G
authors.lastname: Velazquez
authors.affiliation: A Viren SA de CV, Presidente Benito Juárez 110B, José María Arteaga, Querétaro, Querétaro. 76135, México.
authors.forename: Isidro
authors.initials: I
authors.lastname: Fernandez-Siurob
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology; D002645:Chickens; D016825:Chlamydomonas reinhardtii / Q000235:genetics* / Q000378:metabolism; D002736:Chloroplasts / Q000235:genetics / Q000378:metabolism; D004353:Drug Evaluation, Preclinical; D015870:Gene Expression; D019267:Hemagglutinin Glycoproteins, Influenza Virus / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*; D053125:Influenza A Virus, H5N2 Subtype / Q000235:genetics / Q000276:immunology*; D007252:Influenza Vaccines / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*; D005585:Influenza in Birds / Q000276:immunology* / Q000517:prevention & control / Q000821:virology; D011201:Poultry Diseases / Q000276:immunology* / Q000517:prevention & control / Q000821:virology
chemical_list: D000914:Antibodies, Viral; D019267:Hemagglutinin Glycoproteins, Influenza Virus; D007252:Influenza Vaccines
keywords: Chlamydomonas reinhardtii; chicken; chloroplast; hemagglutinin; influenza A virus; recombinant protein