title: The Biology of β-D-mannuronic acid (M2000) on Human Dendritic Cell Based on MicroRNA-155 and MicroRNA-221.
abstract: The aim of this study was to evaluate the effect of ß-Dmannuronic acid (M2000) on related miRNAs to dendritic cells (DCs) differentiation. DC-based immunosuppressive drugs can suppress the progression of autoimmune diseases, however, their notable side effects in increasing the risk of infectious diseases and cancers should be considered. The β-D-mannuronic acid, as a novel non-steroidal anti-inflammatory agent, has been tested in various experimental models.
  
  
  
  The effect of M2000 on expression of miRNA-155 and miRNA-221 was examined. To investigate how M2000 affects differentiation of human dendritic DCs in a defined inflammatory environment, human peripheral blood mononuclear cells were isolated from healthy blood and the monocytes were purified using anti-CD14 microbeads. The so isolated monocytes were subsequently incubated in the presence of M2000 in two different doses (3 and 6 mMol/well) adding granulocyte-macrophage colony-stimulating factor (GM-CSF) and interleukin-4 for inducing monocytes to immature DC and lipopolysaccharide for running DC differentiation. The expression of miRNA-155 and miRNA-221 were examined with Real Time PCR.
  
  
  
  The results demonstrate that M2000 has no significant side effect on expression of miR-155 and miR-221 in both immature DC and mature DC process in vitro.
  
  
  
  Our findings show that β-D-mannuronic acid is a safe agent which has no adverse effect on regulatory miRNA-155 and miRNA-221 in dendritic cells.
authors.forename: Nakisa
authors.initials: N
authors.lastname: Tabrizian
authors.forename: Nazanin Arjomand
authors.initials: NA
authors.lastname: Fard
authors.forename: Ali
authors.initials: A
authors.lastname: Farazmand
authors.forename: Rahil
authors.initials: R
authors.lastname: Eftekhari
authors.forename: Farzaneh Tofighi
authors.initials: FT
authors.lastname: Zavareh
authors.affiliation: Department of Immunology, School of Public Health, Tehran University of Medical Sciences, Box: 14155-6446, Tehran, Iran.
authors.forename: Abbas
authors.initials: A
authors.lastname: Mirshafiey
mesh_terms: D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology*; D002454:Cell Differentiation / Q000187:drug effects; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000166:cytology / Q000187:drug effects* / Q000502:physiology; D006603:Hexuronic Acids / Q000494:pharmacology*; D006801:Humans; D007166:Immunosuppressive Agents / Q000494:pharmacology*; D035683:MicroRNAs / Q000235:genetics*
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D006603:Hexuronic Acids; D007166:Immunosuppressive Agents; D035683:MicroRNAs; C524942:MIRN155 microRNA, human; C525709:MIR221, human; C008324:mannuronic acid
keywords: DCs; M2000; dendritic cells; miRNA-155; miRNA-221; β-D-mannuronic acid