title: Multicolor Colormetric Biosensor for the Determination of Glucose based on the Etching of Gold Nanorods.
abstract: In this work, 3,3',5,5'-tetramethylbenzidine(II) (TMB2+), derived from H2O2-horseradish peroxidase (HRP)-3,3',5,5'-tetramethylbenzidine (H2O2-HRP-TMB) reaction system, was used to etch AuNRs to generate different colors of solution. Many enzyme reactions are involved in the production of H2O2(e.g., glucose can react with the dissolved oxygen in the presence of glucose oxidase (GOx) to produce H2O2). Given this information, a simple visual biosensor was developed in this study, with glucose as the example target. The detection range of the proposed system varied with the experimental conditions, such as the concentration of GOx and HRP, and enzymatic reaction time. Under the optimized conditions, the longitudinal shift of localized surface plasmon resonances (LSPR) had a linear correlation with the glucose concentration in the range of 0.1~1.0 mM. Meanwhile, the solution displayed a specific color in response to the glucose concentration, thus enabling the visual quantitative detection of glucose at a glance. Compared with the traditional monochromic colorimetry, this multicolor glucose sensor generates various vivid colors, which can be easily distinguished by naked eyes without any sophisticated instrument. Notably, the proposed method has been successfully applied to detect glucose in serum samples with satisfied results.
authors.affiliation: MOE Key Laboratory of Analysis and Detection for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, Institute of Nanomedicine and Nanobiosensing, College of Chemistry, Fuzhou University, Fuzhou, Fujian 350116, China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Lin
authors.affiliation: MOE Key Laboratory of Analysis and Detection for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, Institute of Nanomedicine and Nanobiosensing, College of Chemistry, Fuzhou University, Fuzhou, Fujian 350116, China.
authors.forename: Mengmeng
authors.initials: M
authors.lastname: Zhao
authors.affiliation: MOE Key Laboratory of Analysis and Detection for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, Institute of Nanomedicine and Nanobiosensing, College of Chemistry, Fuzhou University, Fuzhou, Fujian 350116, China.
authors.forename: Yajuan
authors.initials: Y
authors.lastname: Guo
authors.affiliation: MOE Key Laboratory of Analysis and Detection for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, Institute of Nanomedicine and Nanobiosensing, College of Chemistry, Fuzhou University, Fuzhou, Fujian 350116, China.
authors.forename: Xiaoming
authors.initials: X
authors.lastname: Ma
authors.affiliation: College of Biological Science and Technology, Fuzhou University, Fuzhou, Fujian 350116, China.
authors.forename: Fang
authors.initials: F
authors.lastname: Luo
authors.affiliation: MOE Key Laboratory of Analysis and Detection for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, Institute of Nanomedicine and Nanobiosensing, College of Chemistry, Fuzhou University, Fuzhou, Fujian 350116, China.
authors.forename: Longhua
authors.initials: L
authors.lastname: Guo
authors.affiliation: MOE Key Laboratory of Analysis and Detection for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, Institute of Nanomedicine and Nanobiosensing, College of Chemistry, Fuzhou University, Fuzhou, Fujian 350116, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Qiu
authors.affiliation: MOE Key Laboratory of Analysis and Detection for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, Institute of Nanomedicine and Nanobiosensing, College of Chemistry, Fuzhou University, Fuzhou, Fujian 350116, China.
authors.forename: Guonan
authors.initials: G
authors.lastname: Chen
authors.affiliation: MOE Key Laboratory of Analysis and Detection for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, Institute of Nanomedicine and Nanobiosensing, College of Chemistry, Fuzhou University, Fuzhou, Fujian 350116, China.
authors.forename: Zhenyu
authors.initials: Z
authors.lastname: Lin
mesh_terms: D015374:Biosensing Techniques; D005947:Glucose / Q000737:chemistry*; D006046:Gold / Q000737:chemistry*; D006735:Horseradish Peroxidase / Q000378:metabolism; D043942:Nanotubes / Q000737:chemistry*; D020349:Surface Plasmon Resonance
chemical_list: D006046:Gold; D006735:Horseradish Peroxidase; D005947:Glucose