title: Concentrations of thromboxane metabolites in feline urine.
abstract: OBJECTIVE To determine the predominant thromboxane (TX) metabolite in urine of healthy cats, evaluate whether the method of sample collection would impact concentration of that metabolite, and propose a reference interval for that metabolite in urine of healthy cats. ANIMALS 17 cats (11 purpose-bred domestic shorthair cats, 5 client-owned domestic shorthair cats, and 1 client-owned Persian cat). PROCEDURES All cats were deemed healthy on the basis of results for physical examination, a CBC, serum biochemical analysis, urinalysis, and measurement of prothrombin time and activated partial thromboplastin time. Voided and cystocentesis urine samples (or both) were collected. Aliquots of urine were stored at -80°C until analysis. Concentrations of TXB2, 11-dehydroTXB2, and 2,3 dinorTXB2were measured with commercially available ELISA kits. Urinary creatinine concentration was also measured. RESULTS 11-dehydroTXB2was the most abundant compound, representing (mean ± SD) 59 ± 18% of the total amount of TX detected. In all samples, the concentration of 11-dehydroTXB2was greater than that of 2,3 dinorTXB2(mean, 4.2 ± 2.7-fold as high). Mean concentration of 11-dehydroTXB2for the 17 cats was 0.57 ± 0.47 ng/mg of creatinine. A reference interval (based on the 5% to 95% confidence interval) of 0.10 to 2.1 ng of 11-dehydroTXB2/mg of creatinine was proposed for healthy cats. CONCLUSIONS AND CLINICAL RELEVANCE In this study, 11-dehydroTXB2was the major TX metabolite in feline urine. Measurement of this metabolite may represent a noninvasive, convenient method for monitoring in vivo platelet activation in cats at risk for thromboembolism.
authors.forename: Brittany
authors.initials: B
authors.lastname: Heggem-Perry
authors.forename: Stephanie A
authors.initials: SA
authors.lastname: Smith
authors.forename: Maureen A
authors.initials: MA
authors.lastname: McMichael
authors.forename: Mauria
authors.initials: M
authors.lastname: O'Brien
authors.forename: Anne
authors.initials: A
authors.lastname: Saunders
authors.forename: Jason
authors.initials: J
authors.lastname: Tarricone
mesh_terms: D000818:Animals; D002415:Cats / Q000652:urine*; D003404:Creatinine / Q000652:urine*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D008297:Male; D012016:Reference Values; D013048:Specimen Handling; D013931:Thromboxanes / Q000652:urine*; D016482:Urinalysis / Q000662:veterinary
chemical_list: D013931:Thromboxanes; D003404:Creatinine