title: Identification and molecular characterization of Escherichia coli blaSHVgenes in a Chinese teaching hospital.
abstract: Escherichia coli (E. coli) commonly reside in human intestine and most E. coli strains are harmless, but some serotypes cause serious food poisoning. This study identified and molecularly characterized blaSHVgenes from 490 E. coli strains with multi-drug resistance in a hospital population. PCR and molecular cloning and southern blot were performed to assess functions and localizations of this resistant E. coli gene and the pulsed-field gel electrophoresis (PFGE) was utilized to demonstrate the clonal relatedness of the positive E. coli strains. The data showed that 4 of these 490 E. coli strains (4/499, 0.8%) carried blaSHVgenes that included EC D2485 (blaSHV-5), EC D2487 (blaSHV-5), EC D2684 (blaSHV-11) and EC D2616 (blaSHV-195,a novel blaSHV). Analysis of blaSHVopen-reading frame showed that blaSHV-5had a high hydrolysis activity to the broad-spectrum penicillin (ampicillin or piperacillin), ceftazidime, ceftriaxone, cefotaxime and aztreonam. blaSHV-195and blaSHV-11had similar resistant characteristics with high hydrolysis activities to ampicillin and piperacillin, but low activities to cephalosporins. Moreover, the two blaSHV-5genes were located on a transferable plasmid (23kb), whereas the other two blaSHVvariants (blaSHV-11and blaSHV-195) seemed to be located in the chromosomal material. Both EC D2485 and EC D2487 clones isolated in 2010 had the same DNA finger printing profile and they might be the siblings of clonal dissemination. The data from the current study suggest that the novel blaSHVand clonal dissemination may be developed, although blaSHVgenes were infrequently identified in this hospital population. The results of the work demonstrate the necessity for molecular surveillance in tracking blaSHV-producing strains in large teaching hospital settings and emphasize the need for epidemiological monitoring.
authors.affiliation: School of Laboratory Medicine and Life Science/Institute of Biomedical Informatics, Wenzhou Medical University, Wenzhou 325000, China.
authors.forename: Mei
authors.initials: M
authors.lastname: Zhu
authors.affiliation: School of Laboratory Medicine and Life Science/Institute of Biomedical Informatics, Wenzhou Medical University, Wenzhou 325000, China.
authors.forename: Guangjian
authors.initials: G
authors.lastname: Yang
authors.affiliation: School of Laboratory Medicine and Life Science/Institute of Biomedical Informatics, Wenzhou Medical University, Wenzhou 325000, China.
authors.forename: Ailing
authors.initials: A
authors.lastname: Li
authors.affiliation: School of Laboratory Medicine and Life Science/Institute of Biomedical Informatics, Wenzhou Medical University, Wenzhou 325000, China.
authors.forename: Li
authors.initials: L
authors.lastname: Zong
authors.affiliation: Shanghai Public Health Clinical Center, Shanghai 201508, China.
authors.forename: Zhaoguang
authors.initials: Z
authors.lastname: Dong
authors.affiliation: School of Medicine, Lishui College, Lishui 323000, China.
authors.forename: Junwan
authors.initials: J
authors.lastname: Lu
authors.affiliation: School of Medicine, Lishui College, Lishui 323000, China.
authors.forename: Kaibo
authors.initials: K
authors.lastname: Zhang
authors.affiliation: School of Medicine, Lishui College, Lishui 323000, China.
authors.forename: Cong
authors.initials: C
authors.lastname: Cheng
authors.affiliation: School of Laboratory Medicine and Life Science/Institute of Biomedical Informatics, Wenzhou Medical University, Wenzhou 325000, China.
authors.forename: Qingli
authors.initials: Q
authors.lastname: Chang
authors.affiliation: Wenling Women's ﹠ Children's Hospital, Wenling 317500, China.
authors.forename: Xiuying
authors.initials: X
authors.lastname: Wu
authors.affiliation: School of Laboratory Medicine and Life Science/Institute of Biomedical Informatics, Wenzhou Medical University, Wenzhou 325000, China.
authors.forename: Jianchao
authors.initials: J
authors.lastname: Ying
authors.affiliation: Wenling Women's ﹠ Children's Hospital, Wenling 317500, China.
authors.forename: Xianneng
authors.initials: X
authors.lastname: Li
authors.affiliation: School of Laboratory Medicine and Life Science/Institute of Biomedical Informatics, Wenzhou Medical University, Wenzhou 325000, China.
authors.forename: Li
authors.initials: L
authors.lastname: Ding
authors.affiliation: Wenling Women's ﹠ Children's Hospital, Wenling 317500, China.
authors.forename: Haixiao
authors.initials: H
authors.lastname: Zheng
authors.affiliation: Wenling Women's ﹠ Children's Hospital, Wenling 317500, China.
authors.forename: Junping
authors.initials: J
authors.lastname: Yu
authors.affiliation: School of Laboratory Medicine and Life Science/Institute of Biomedical Informatics, Wenzhou Medical University, Wenzhou 325000, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Ying
authors.affiliation: School of Laboratory Medicine and Life Science/Institute of Biomedical Informatics, Wenzhou Medical University, Wenzhou 325000, China.
authors.forename: Teng
authors.initials: T
authors.lastname: Xu
authors.affiliation: School of Laboratory Medicine and Life Science/Institute of Biomedical Informatics, Wenzhou Medical University, Wenzhou 325000, China.
authors.forename: Huiguang
authors.initials: H
authors.lastname: Yi
authors.affiliation: School of Laboratory Medicine and Life Science/Institute of Biomedical Informatics, Wenzhou Medical University, Wenzhou 325000, China.
authors.forename: Peizhen
authors.initials: P
authors.lastname: Li
authors.affiliation: School of Laboratory Medicine and Life Science/Institute of Biomedical Informatics, Wenzhou Medical University, Wenzhou 325000, China.
authors.forename: Kewei
authors.initials: K
authors.lastname: Li
authors.affiliation: School of Medicine, Lishui College, Lishui 323000, China.
authors.forename: Songquan
authors.initials: S
authors.lastname: Wu
authors.affiliation: School of Laboratory Medicine and Life Science/Institute of Biomedical Informatics, Wenzhou Medical University, Wenzhou 325000, China. Electronic address: baoqy@genomics.cn.
authors.forename: Qiyu
authors.initials: Q
authors.lastname: Bao
authors.affiliation: Wenling Women's ﹠ Children's Hospital, Wenling 317500, China. Electronic address: wangzhe96@163.com.
authors.forename: Junrong
authors.initials: J
authors.lastname: Wang
mesh_terms: D002681:China; D003428:Cross Infection / Q000188:drug therapy / Q000382:microbiology; D024901:Drug Resistance, Multiple, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000187:drug effects* / Q000201:enzymology / Q000235:genetics*; D004927:Escherichia coli Infections / Q000188:drug therapy / Q000382:microbiology; D029968:Escherichia coli Proteins / Q000235:genetics*; D005798:Genes, Bacterial; D005838:Genotype; D006784:Hospitals, Teaching; D006801:Humans; D010802:Phylogeny; D011815:R Factors / Q000235:genetics; D001618:beta-Lactamases / Q000235:genetics*
chemical_list: D029968:Escherichia coli Proteins; D001618:beta-Lactamases
keywords: Clonal dissemination; E. coli; Hospital populations; Multidrug resistance; bla(SHV)