title: Structural Investigation of Cell Wall Xylan Polysaccharides from the Leaves of Algerian Argania spinosa.
abstract: Xylan-type polysaccharides were isolated from the leaves ofArgania spinosa(L.) Skeels collected in the Tindouf area (southwestern Algeria). Xylan fractions were obtained by sequential alkaline extractions and purified on Sepharose CL-4B. The xylan structure was investigated by enzymatic hydrolysis with an endo-β(1→4)-xylanase followed by chromatography of the resulting fragments on Biogel P2, characterization by sugar analysis and matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS ). The results show that theA. spinosaxylan is composed of a β-(1→4)-d-xylopyranose backbone substituted with 4-O-methyl-d-glucuronic acid and L-arabinose residues.
authors.affiliation: Laboratoire de Biotoxicologie, Pharmacognosie et Valorisation Biologique des Plantes (LBPVBP), Département de biologie, Faculté des sciences, Université Dr. Tahar Moulay de Saida, BP 138 cité ENNASR, 20000 Saida, Algérie. kadda46@hotmail.com.|Laboratoire des Productions, Valorisations Végétales et Microbiennes (LP2VM), Département de Biotechnologie, Faculté des sciences de la nature et de la vie, Université des Sciences et de la Technologie d'Oran, BP 1505 El M'Naouar, 31000 Oran, Algérie. kadda46@hotmail.com.
authors.forename: Kadda
authors.initials: K
authors.lastname: Hachem
authors.affiliation: Laboratoire de Chimie des Substances Naturelles (EA1069), Faculté des Sciences et Techniques, Université de Limoges, 123 avenue Albert Thomas, 87060 Limoges, France. celine.faugeron@unilim.fr.
authors.forename: Céline
authors.initials: C
authors.lastname: Faugeron
authors.affiliation: Laboratoire des Productions, Valorisations Végétales et Microbiennes (LP2VM), Département de Biotechnologie, Faculté des sciences de la nature et de la vie, Université des Sciences et de la Technologie d'Oran, BP 1505 El M'Naouar, 31000 Oran, Algérie. kaidharche@yahoo.fr.
authors.forename: Meriem
authors.initials: M
authors.lastname: Kaid-Harche
authors.affiliation: Laboratoire de Chimie des Substances Naturelles (EA1069), Faculté des Sciences et Techniques, Université de Limoges, 123 avenue Albert Thomas, 87060 Limoges, France. vincent.gloaguen@unilim.fr.
authors.forename: Vincent
authors.initials: V
authors.lastname: Gloaguen
mesh_terms: D002473:Cell Wall / Q000737:chemistry*; D005591:Chemical Fractionation; D006868:Hydrolysis; D015394:Molecular Structure; D009005:Monosaccharides / Q000737:chemistry; D010936:Plant Extracts / Q000737:chemistry; D018515:Plant Leaves / Q000737:chemistry*; D058105:Polymerization; D029632:Sapotaceae / Q000737:chemistry*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014990:Xylans / Q000737:chemistry*
chemical_list: D009005:Monosaccharides; D010936:Plant Extracts; D014990:Xylans
keywords: Argania spinosa; MALDI-TOF MS; leaf; xylan