title: Acidicapsa acidisoli sp. nov., from the acidic soil of a deciduous forest.
abstract: A bacterial strain designated strain SK-11Twas isolated from the acidic soil of a deciduous forest in the Shirakami Mountains in Japan. Cells of strain SK-11Twere aerobic, non-motile, Gram-stain-negative rods, 0.7-1.0 µm in width and 1.0-1.4 µm in length. The pH range for growth was between pH 4.0 and 5.5, with an optimum at pH 5.0. The temperature range for growth was between 10 and 35 °C, with an optimum at around 25-30 °C. Strain SK-11Tutilized various carbohydrates as growth substrates as well as yeast extract and protein hydrolysates. The major cellular fatty acids (>10 % of total fatty acid contents) were iso-C15 : 0 (55.4 %), iso-C17 : 0 (16.7 %) and iso-C17 : 1ω9c/10 methyl-hexadecanoic acid (17.7 %). The major respiratory quinone was MK-8. The polar lipids consisted of phosphatidylethanolamine, phosphatidylglycerol, two unidentified phospholipids and an unidentified polar lipid. The DNA G+C content of strain SK-11Twas 56.9 %. Phylogenetic analysis based on the 16S rRNA gene sequences revealed that strain SK-11Tbelonged to the family Acidobacteriaceae within subdivision 1 of the phylum Acidobacteria, and the closest relatives of strain SK-11Twere Acidicapsa ligni WH120Tand Acidicapsa borealis KA1T, with 16S rRNA gene sequence similarities of 96.6 and 96.5 %, respectively. On the basis of the evidence from our polyphasic study, we concluded that strain SK-11Trepresents a novel species of the genus Acidicapsa, and propose the name Acidicapsa acidisoli sp. nov. The type strain of Acidicapsaacidisolisp nov. is SK-11T(=DSM 100508T=NBRC 111227T).
authors.affiliation: Faculty of Agriculture and Life Science, Hirosaki University, 3 Bunkyo-cho, Hirosaki, Aomori 036-8561, Japan.
authors.forename: Heizo
authors.initials: H
authors.lastname: Matsuo
authors.affiliation: Faculty of Agriculture and Life Science, Hirosaki University, 3 Bunkyo-cho, Hirosaki, Aomori 036-8561, Japan.
authors.forename: Chisaki
authors.initials: C
authors.lastname: Kudo
authors.affiliation: Faculty of Agriculture and Life Science, Hirosaki University, 3 Bunkyo-cho, Hirosaki, Aomori 036-8561, Japan.
authors.forename: Juan
authors.initials: J
authors.lastname: Li
authors.affiliation: Faculty of Agriculture and Life Science, Hirosaki University, 3 Bunkyo-cho, Hirosaki, Aomori 036-8561, Japan.
authors.forename: Akio
authors.initials: A
authors.lastname: Tonouchi
mesh_terms: D061271:Acidobacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D065928:Forests; D007564:Japan; D010743:Phospholipids / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; C024223:vitamin MK 8
keywords: Acidobacteria; subdivision I