title: The preparation and characterization of a novel sphingan WL from marine Sphingomonas sp. WG.
abstract: Sphingans, a group of structurally closely related bacterial exopolysaccharides produced by members of the genus Sphingomonas, can be applied in a variety of industries such as food, cement, and personal care applications due to their high viscosity. A high sphingan-producing-bacterium, Sphingomonas sp. WG can secret large quantity of sphingan designated as WL. To enhance the production of WL, a three-stage control strategy was applied and the highest WL production can reach 33.3 g/L. The rheological analysis showed that the aqueous solution of WL had high viscosity, typical shearing-thinning behavior and great stability to high temperature, a wide range of pH (1 to 14), and high salinity. WL was composed principally of carbohydrate with 6.52% O-acyl groups. The carbohydrate portion of WL contained about 13% glucuronic acid and some neutral sugars including mannose, glucose and rhamnose in the molar ratio of 1:2.28:2.12. Partial acid hydrolysis of WL produced a new oligosaccharide WL-1. Structural resolution revealed that WL-1 consisted of α-L-Rha-(1→4)-β-L-Rha-(1→4)-β-D-Glc-(1→3)-α-D-Glc with β-D-Man substituent at the third glucose residue and carboxyl and O-acyl groups. These findings will broaden the applications of this novel sphingan in food, ink, oil and other industries.
authors.affiliation: Centre for Bioengineering and Biotechnology, China University of Petroleum (East China), 66 Changjiang West Road, Qingdao 266580, People's Republic of China.
authors.forename: Hui
authors.initials: H
authors.lastname: Li
authors.affiliation: Centre for Bioengineering and Biotechnology, China University of Petroleum (East China), 66 Changjiang West Road, Qingdao 266580, People's Republic of China.
authors.forename: Xue
authors.initials: X
authors.lastname: Jiao
authors.affiliation: Centre for Bioengineering and Biotechnology, China University of Petroleum (East China), 66 Changjiang West Road, Qingdao 266580, People's Republic of China.
authors.forename: Yajie
authors.initials: Y
authors.lastname: Sun
authors.affiliation: Centre for Bioengineering and Biotechnology, China University of Petroleum (East China), 66 Changjiang West Road, Qingdao 266580, People's Republic of China.
authors.forename: Shiwei
authors.initials: S
authors.lastname: Sun
authors.affiliation: Centre for Bioengineering and Biotechnology, China University of Petroleum (East China), 66 Changjiang West Road, Qingdao 266580, People's Republic of China.
authors.forename: Zhimei
authors.initials: Z
authors.lastname: Feng
authors.affiliation: Centre for Bioengineering and Biotechnology, China University of Petroleum (East China), 66 Changjiang West Road, Qingdao 266580, People's Republic of China.
authors.forename: Wanlong
authors.initials: W
authors.lastname: Zhou
authors.affiliation: Centre for Bioengineering and Biotechnology, China University of Petroleum (East China), 66 Changjiang West Road, Qingdao 266580, People's Republic of China.
authors.forename: Hu
authors.initials: H
authors.lastname: Zhu
mesh_terms: D059001:Aquatic Organisms; D005285:Fermentation; D006868:Hydrolysis; D007218:Industrial Microbiology / Q000379:methods; D009682:Magnetic Resonance Spectroscopy; D008970:Molecular Weight; D009005:Monosaccharides / Q000032:analysis; D011135:Polysaccharides, Bacterial / Q000096:biosynthesis* / Q000737:chemistry* / Q000302:isolation & purification; D012212:Rheology; D013056:Spectrophotometry, Ultraviolet; D017550:Spectroscopy, Fourier Transform Infrared; D020578:Sphingomonas / Q000378:metabolism*
chemical_list: D009005:Monosaccharides; D011135:Polysaccharides, Bacterial; C430406:sphingan