title: Modern tools for the chemical ligation and synthesis of modified peptides and proteins.
abstract: The ability to improve nature's capacity by introducing modification of biological interest in proteins and peptides (P&P) is one of the modern challenges in synthetic chemistry. Due to the unfavorable pharmacokinetic properties, many native P&P are of little use as therapeutic agents. Today, few methods for the preparation of modified proteins are available. Initially introduced to realize the ligation between two standard peptidic sequences, and hence to afford native proteins, the modern chemical methodologies, in other words native chemical ligation, expressed ligation, Staudinger ligation, auxiliary mediated ligation, aldehyde capture, etc., can be virtually utilized to ligate a variety of peptidomimetic partners, allowing a systematic access to modified, unnatural large P&P.
authors.affiliation: Department of Chemistry 'G. Ciamician', University of Bologna, via Selmi 2, 40126-Bologna, Italy.
authors.forename: Luca
authors.initials: L
authors.lastname: Gentilucci
authors.affiliation: Department of Chemistry 'G. Ciamician', University of Bologna, via Selmi 2, 40126-Bologna, Italy.
authors.forename: Pierluigi
authors.initials: P
authors.lastname: Tosi
authors.affiliation: Department of Chemistry 'G. Ciamician', University of Bologna, via Selmi 2, 40126-Bologna, Italy.
authors.forename: Adriano
authors.initials: A
authors.lastname: Bauer
authors.affiliation: Department of Chemistry 'G. Ciamician', University of Bologna, via Selmi 2, 40126-Bologna, Italy.
authors.forename: Rossella
authors.initials: R
authors.lastname: De Marco
mesh_terms: D015394:Molecular Structure; D010455:Peptides / Q000138:chemical synthesis* / Q000737:chemistry; D011506:Proteins / Q000138:chemical synthesis* / Q000737:chemistry
chemical_list: D010455:Peptides; D011506:Proteins
keywords: native chemical ligation; peptide; protein chemistry