title: Increased expression of long non-coding RNA XIST predicts favorable prognosis of cervical squamous cell carcinoma subsequent to definitive chemoradiation therapy.
abstract: The present retrospective study aimed to examine the association between the expression of long non-protein-coding RNAs (lncRNAs) and clinical prognosis in the pretreatment formalin-fixed, paraffin-embedded (FFPE) tissue samples of cervical squamous cell carcinoma patients that underwent platinum-based chemoradiation therapy. Between 2001 and 2013, 49 consecutive patients with squamous cell cervical carcinoma were selected for the present study (median follow-up period, 44.1 months). The patients possessed an International Federation of Gynecology and Obstetrics stage of IB1/IIA1 (with pelvic lymph node metastasis), IB2 or IIA2-IVA, and had been treated with definitive chemoradiation therapy. The pretreatment FFPE tumor biopsies of the patients obtained diagnosis were used for analysis. Total RNAs were extracted from the FFPE tumor tissues and reverse transcription-quantitative polymerase chain reaction was performed to examine the expression level of lncRNAs. The expression level of X inactive-specific transcript (XIST) demonstrated a significant association with the overall survival rate (P=0.014). The 4-year overall survival rates were 87.1 and 54.4% in the high and low XIST expression groups, respectively. Since the expression of XIST is associated with the overall survival rate, this lncRNA has the potential to become a predictor for the prognosis of cervical squamous cell carcinoma patients that are treated with chemoradiation therapy. Additional studies are required to investigate the underlying mechanisms of XIST that are associated with prognosis.
authors.affiliation: Department of Radiology, Graduate School of Medicine, University of Tokyo, Tokyo 113-8655, Japan.
authors.forename: Reiko
authors.initials: R
authors.lastname: Kobayashi
authors.affiliation: Department of Pathology, Graduate School of Medicine, University of Tokyo, Tokyo 113-8655, Japan.
authors.forename: Ryu
authors.initials: R
authors.lastname: Miyagawa
authors.affiliation: Department of Radiology, Graduate School of Medicine, University of Tokyo, Tokyo 113-8655, Japan.
authors.forename: Hideomi
authors.initials: H
authors.lastname: Yamashita
authors.affiliation: Department of Pathology, Graduate School of Medicine, University of Tokyo, Tokyo 113-8655, Japan.
authors.forename: Teppei
authors.initials: T
authors.lastname: Morikawa
authors.affiliation: Department of Radiology, Graduate School of Medicine, University of Tokyo, Tokyo 113-8655, Japan.
authors.forename: Kae
authors.initials: K
authors.lastname: Okuma
authors.affiliation: Department of Pathology, Graduate School of Medicine, University of Tokyo, Tokyo 113-8655, Japan.
authors.forename: Masashi
authors.initials: M
authors.lastname: Fukayama
authors.affiliation: Department of Radiology, Graduate School of Medicine, University of Tokyo, Tokyo 113-8655, Japan.
authors.forename: Kuni
authors.initials: K
authors.lastname: Ohtomo
authors.affiliation: Department of Radiology, Graduate School of Medicine, University of Tokyo, Tokyo 113-8655, Japan.
authors.forename: Keiichi
authors.initials: K
authors.lastname: Nakagawa
keywords: XIST; cervical cancer; long non-coding RNA