title: Highly improved acarbose production of Actinomyces through the combination of ARTP and penicillin susceptible mutant screening.
abstract: Atmospheric and room temperature plasma (ARTP) was first employed to generate mutants of Actinomyces JN537 for improving acarbose production. To obtain higher acarbose producing strains, the method of screening the strains for susceptibility to penicillin was used after treatment with ARTP. The rationale for the strategy was that mutants showing penicillin susceptibility were likely to be high acarbose producers, as their ability to synthesize cell walls was weak which might enhance metabolic flux to the pathway of acarbose biosynthesis. Acarbose yield of the mutant strain M37 increased by 62.5 % than that of the original strain. The contents of monosaccharides and amino acids of the cell wall of M37 were lower than that of the original strain. The acarbose production ability in mutant strain remained relatively stable after 10 generations. This work provides a promising strategy for obtaining high acarbose-yield strains by combination of ARTP mutation method and efficient screening technique.
authors.affiliation: State Key Laboratory of Food Science and Technology, Jiangnan University, 1800 Lihu Road, Wuxi, 214122, Jiangsu, China. renfei@swust.edu.cn.|School of Life Science and Engineering, Southwest University of Science and Technology, 59 Qinglong Road, Mianyang, 621010, Sichuan, China. renfei@swust.edu.cn.
authors.forename: Fei
authors.initials: F
authors.lastname: Ren
authors.affiliation: State Key Laboratory of Food Science and Technology, Jiangnan University, 1800 Lihu Road, Wuxi, 214122, Jiangsu, China.
authors.forename: Long
authors.initials: L
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Food Science and Technology, Jiangnan University, 1800 Lihu Road, Wuxi, 214122, Jiangsu, China. tqyjn@163.com.
authors.forename: Qunyi
authors.initials: Q
authors.lastname: Tong
mesh_terms: D020909:Acarbose / Q000378:metabolism*; D000190:Actinomyces / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D001272:Atmosphere; D002473:Cell Wall / Q000187:drug effects; D005800:Genes, Fungal; D024861:Genetic Enhancement; D065089:Glycoside Hydrolase Inhibitors; D006863:Hydrogen-Ion Concentration; D009154:Mutation; D010406:Penicillins / Q000494:pharmacology*; D058626:Plasma Gases / Q000494:pharmacology*; D013696:Temperature
chemical_list: D065089:Glycoside Hydrolase Inhibitors; D010406:Penicillins; D058626:Plasma Gases; D020909:Acarbose
keywords: ARTP; Acarbose; Actinomyces; Cell wall components; Mutation breeding