title: Role of NCAN rs2228603 polymorphism in the incidence of nonalcoholic fatty liver disease: a case-control study.
abstract: BACKGROUND
  Recently genome-wide association studies identified that NCAN rs2228603 polymorphism was associated with non-alcoholic fatty liver disease (NAFLD) mainly in subjects of European ancestry. While no research have been conducted to demonstrate the relationship between NCAN rs2228603 and NAFLD in Chinese Han adults. The aim of this study was to investigate whether NCAN rs2228603 is associated with NAFLD in Chinese population.
  
  
  METHODS
  Gene NCAN rs2228603 was genotyped in 182 patients with NAFLD and 195 healthy controls. The expression of NCAN was tested according to polymerase chain reaction analysis (PCR) and serum lipids were performed by biology techniques.
  
  
  RESULTS
  No significant difference was found in genotype and allele frequencies of NCAN rs2228603 between the NAFLD group and the controls (P > 0.05). Subjects with the NCAN rs2228603 CT genotype showed a higher level of alkaline phosphatase (AKP) (P = 0.017) and a higher high-density lipoprotein (HDL) (P < 0.05).
  
  
  CONCLUSIONS
  Our study for the first time identified that the gene NCAN rs2228603 is not a risk factor for the incidence of NAFLD in Chinese population. Also we found the dual and opposite role of T variant in protecting liver with a higher level of HDL and conferring risk for liver damage with a higher level of AKP.
  
  
  BACKGROUND
  Chinese Clinical Trial Register.gov Identifier: ChiCTR-ROC-15006447 .
authors.affiliation: Department of Gastroenterology, Qingdao Municipal Hospital, Nanjing Medical University, Qingdao, 266071, Shandong Province, China.
authors.forename: Meng-Juan
authors.initials: MJ
authors.lastname: Wu
authors.affiliation: Department of Gastroenterology, Qingdao Municipal Hospital, Nanjing Medical University, Qingdao, 266071, Shandong Province, China.
authors.forename: Chen
authors.initials: C
authors.lastname: Yuan
authors.affiliation: Digestive Disease Key Laboratory of Qingdao, Qingdao, Shandong Province, China.|Central Laboratories, Qingdao Municipal Hospital, Qingdao, Shandong Province, China.
authors.forename: Lin-Lin
authors.initials: LL
authors.lastname: Lu
authors.affiliation: Department of Gastroenterology, Qingdao Municipal Hospital, Nanjing Medical University, Qingdao, 266071, Shandong Province, China.
authors.forename: Bai-Quan
authors.initials: BQ
authors.lastname: An
authors.affiliation: Department of Gastroenterology, Qingdao Municipal Hospital, Nanjing Medical University, Qingdao, 266071, Shandong Province, China. xuansydxy@163.com.|Department of Gastroenterology, Qingdao Municipal Hospital, Qingdao, Shandong Province, China. xuansydxy@163.com.
authors.forename: Shi-Ying
authors.initials: SY
authors.lastname: Xuan
authors.affiliation: Department of Gastroenterology, Qingdao Municipal Hospital, Nanjing Medical University, Qingdao, 266071, Shandong Province, China. xinyongning@163.com.|Department of Gastroenterology, Qingdao Municipal Hospital, Qingdao, Shandong Province, China. xinyongning@163.com.
authors.forename: Yong-Ning
authors.initials: YN
authors.lastname: Xin
mesh_terms: D000328:Adult; D044466:Asian People / Q000235:genetics; D016022:Case-Control Studies; D011508:Chondroitin Sulfate Proteoglycans / Q000235:genetics*; D005260:Female; D020022:Genetic Predisposition to Disease; D006801:Humans; D015994:Incidence; D037181:Lectins, C-Type / Q000235:genetics*; D008075:Lipoproteins, HDL / Q000097:blood / Q000235:genetics; D008297:Male; D008875:Middle Aged; D009419:Nerve Tissue Proteins / Q000235:genetics*; D058580:Neurocan; D065626:Non-alcoholic Fatty Liver Disease / Q000097:blood / Q000453:epidemiology* / Q000235:genetics* / Q000378:metabolism; D020641:Polymorphism, Single Nucleotide
chemical_list: D011508:Chondroitin Sulfate Proteoglycans; D037181:Lectins, C-Type; D008075:Lipoproteins, HDL; D009419:Nerve Tissue Proteins; D058580:Neurocan; C076817:NCAN protein, human
keywords: NCAN; Non-alcoholic fatty liver disease; Polymorphism; rs2228603