title: Evidence-based validation of quorum quenching from Lysinibacillus sphaericus and Geobacillus sp. in bioremediation of oil sludge.
abstract: Many studies on quorum quenching focus on the discovery and characterization of novel acyl-homoserine lactonases (AHL-lactonases) because these enzymes could be used in the control of diseases caused by Gram-negative bacteria. The effects of quorum quenching are also remarkable in the performance of bacterial consortia in applications such as bioremediation. In the current work, we demonstrated the presence of a potential novel AHL-lactonase-encoding locus (Bsph_3377) from Lysinibacillus sphaericus and Geobacillus sp. The deduced amino acid sequences for this enzyme possess the characteristic domains and motifs involved in Zn-binding from AHL lactonases and were grouped into 1 clade within the phylogeny of the lactonases from firmicutes, showing 70% of identity with the lactonase AhlS from Solibacillus silvestris. We demonstrated the locus transcription by RT-qPCR and its relationship with the suppression of the pathogenicity of Pectobacterium carotovorum. Additionally, we analyzed the interaction of these bacilli with a commercial consortium in the bioremediation of a hydrocarbon-contaminated soil, showing inhibitory effects on its establishment. These results represent a new contribution in the understanding of the potential biotechnological applications of L. sphaericus and Geobacillus sp. as well as in the research on antibacterial techniques based on quorum-sensing disruption.
authors.affiliation: Centro de Investigaciones Microbiológicas (CIMIC), Universidad de los Andes, Cra 1 No. 18A-10, Colombia.|Centro de Investigaciones Microbiológicas (CIMIC), Universidad de los Andes, Cra 1 No. 18A-10, Colombia.
authors.forename: Camilo
authors.initials: C
authors.lastname: Gómez-Garzón
authors.affiliation: Centro de Investigaciones Microbiológicas (CIMIC), Universidad de los Andes, Cra 1 No. 18A-10, Colombia.|Centro de Investigaciones Microbiológicas (CIMIC), Universidad de los Andes, Cra 1 No. 18A-10, Colombia.
authors.forename: Jenny
authors.initials: J
authors.lastname: Dussán
mesh_terms: D001407:Bacillus / Q000201:enzymology / Q000235:genetics / Q000378:metabolism*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D001673:Biodegradation, Environmental; D002265:Carboxylic Ester Hydrolases / Q000235:genetics / Q000378:metabolism; D056506:Geobacillus / Q000201:enzymology / Q000235:genetics / Q000502:physiology*; D053038:Quorum Sensing; D012722:Sewage / Q000382:microbiology*
chemical_list: D001426:Bacterial Proteins; D012722:Sewage; D002265:Carboxylic Ester Hydrolases; C020579:gluconolactonase
keywords: AHL lactonase; Geobacillus; Lysinibacillus sphaericus; bioremediation; biorestauration; désactivation du quorum; quorum quenching