title: No Association between TNF-α -308G/A Polymorphism and Idiopathic Recurrent Miscarriage: A Systematic Review with Meta-Analysis and Trial Sequential Analysis.
abstract: BACKGROUND
  Conflicting results were reported on the association between the TNF-α -308G/A polymorphism and idiopathic recurrent miscarriage (IRM). Though three meta-analyses have been conducted on this topic, the conclusions were contradictory, and the results may be unreliable as certain crucial conditions were neglected.
  
  
  METHODS
  A complete search was conducted in PubMed, Cochrane Library, and Embase, other sources like Google Scholar, ClinicalTrial.gov and reference lists of relevant articles were also retrieved. All candidate articles were accessed and screened using specific inclusion and exclusion criteria. Statistical analyses were performed on data extracted from eligible studies using the STATA 12.0 software and the TSA 0.9 beta software.
  
  
  RESULTS
  Eventually, 12 case-control studies from 11 publications (with 1,807 cases and 2,012 controls) were included in this meta-analysis, and no evidence of any significant association was found in the overall analyses between the TNF-α -308G/A polymorphism and IRM risk. However, significant association was shown in Asian population (four studies from three publications) in the dominant model (AA + GA vs. GG), the allelic model (A vs. G), and the heterozygote model (GA vs. GG).
  
  
  CONCLUSIONS
  TNF-α -308G/A polymorphism is not associated with IRM risk. Though significant association was found in Asian population, the result needs further confirmation from more studies.
authors.affiliation: Department of Clinical Laboratory, the Fourth Affiliated Hospital of Guangxi Medical University, Liuzhou, Guangxi, China.
authors.forename: Jiashu
authors.initials: J
authors.lastname: Dong
authors.affiliation: Department of Clinical Laboratory, the Fourth Affiliated Hospital of Guangxi Medical University, Liuzhou, Guangxi, China.
authors.forename: Jinwan
authors.initials: J
authors.lastname: Li
authors.affiliation: Department of Clinical Laboratory, the Fourth Affiliated Hospital of Guangxi Medical University, Liuzhou, Guangxi, China.
authors.forename: Gechen
authors.initials: G
authors.lastname: Zhou
authors.affiliation: Department of Clinical Laboratory, the Fourth Affiliated Hospital of Guangxi Medical University, Liuzhou, Guangxi, China.
authors.forename: Zheng
authors.initials: Z
authors.lastname: Peng
authors.affiliation: Department of Gynaecology and Obstetrics, the Fourth Affiliated Hospital of Guangxi Medical University, Liuzhou, Guangxi, China.
authors.forename: Jingjing
authors.initials: J
authors.lastname: Li
authors.affiliation: Department of Clinical Laboratory, the Fourth Affiliated Hospital of Guangxi Medical University, Liuzhou, Guangxi, China.
authors.forename: Shengzhang
authors.initials: S
authors.lastname: Lin
authors.affiliation: Department of Clinical Laboratory, the Fourth Affiliated Hospital of Guangxi Medical University, Liuzhou, Guangxi, China.
authors.forename: Haihua
authors.initials: H
authors.lastname: Liu
authors.affiliation: Department of Clinical Laboratory, the Fourth Affiliated Hospital of Guangxi Medical University, Liuzhou, Guangxi, China.
authors.forename: Chunlin
authors.initials: C
authors.lastname: Wu
authors.affiliation: Department of Clinical Laboratory, the Fourth Affiliated Hospital of Guangxi Medical University, Liuzhou, Guangxi, China.
authors.forename: Yujie
authors.initials: Y
authors.lastname: Huang
authors.affiliation: Department of Clinical Laboratory, Liuzhou Maternity and Child Health Care Hospital, Liuzhou, Guangxi, China.
authors.forename: Xiaolan
authors.initials: X
authors.lastname: Lv
authors.affiliation: Department of Clinical Laboratory, the Fourth Affiliated Hospital of Guangxi Medical University, Liuzhou, Guangxi, China.
authors.forename: Shengming
authors.initials: S
authors.lastname: Dai
mesh_terms: D000026:Abortion, Habitual / Q000235:genetics*; D005260:Female; D020022:Genetic Predisposition to Disease; D006801:Humans; D020641:Polymorphism, Single Nucleotide; D011247:Pregnancy; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics*
chemical_list: D014409:Tumor Necrosis Factor-alpha