title: Ethanol extracts fromPortulaca oleraceaL. attenuated ischemia/reperfusion induced rat neural injury through inhibition of HMGB1 induced inflammation.
abstract: It is well demonstrated that the high mobility group box 1 (HMGB1) mediated inflammation has been implicated as one of the important causes for brain damage induced by cerebral ischemia/reperfusion (I/R). In the present study, we assessed the neuro-protective and anti-inflammation effects of the ethanol extracts fromPortulaca oleracea L.(EEPO) against cerebral I/R injury in the rat transient middle cerebral artery occlusion (tMCAO) model. Rats were administrated with their respective treatment for 7 days before the MCA occlusion. After that, rats were intraperitoneal injection with chloral hydrate and sacrificed by decapitation, then the serum and brain tissue were collected. The neurological deficit score, infarct size and brain edema were tested. The levels of serum cytokine as TNF-α, IL-1β, INF-γ, IL-6, and HMGB1 and LDH were detected. The protein level of tissue or nucleus HMGB1, IκB and p-p65 were tested, too. The results showed that pretreatment with EEPO significantly decreased the neurological deficit score, infarct size and brain edema. Moreover, EEPO decreased rat serum cytokine level and rat right cortices p-p65 and IκB protein level. In conclusion all these results suggested that pretreatment with EEFPO provided significant protection against cerebral I/R injury in rats might by virtue of its anti-inflammation property through inhibition of increase of neuleus HMGB1.
authors.affiliation: Faculty of Naval Medicine, Second Military Medical University Shanghai, China.
authors.forename: Chenggang
authors.initials: C
authors.lastname: Zheng
authors.affiliation: Eastern Hepatobiliary Surgery Institute Shanghai, China.
authors.forename: Chen
authors.initials: C
authors.lastname: Liu
authors.affiliation: Faculty of Naval Medicine, Second Military Medical University Shanghai, China.
authors.forename: Wanyin
authors.initials: W
authors.lastname: Wang
authors.affiliation: Department of Hematology, Changhai Hospital, Second Military Medical University Shanghai, China.
authors.forename: Gusheng
authors.initials: G
authors.lastname: Tang
authors.affiliation: Eastern Hepatobiliary Surgery Institute Shanghai, China.
authors.forename: Liwei
authors.initials: L
authors.lastname: Dong
authors.affiliation: Department of Pharmacology, Second Military Medical University Shanghai, China.
authors.forename: Juan
authors.initials: J
authors.lastname: Zhou
authors.affiliation: Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of MedicineShanghai, China; Department of The Clinical Laboratory, The First Affiliated Hospital of Bengbu Medical CollegeAnhui, China.
authors.forename: Zhengrong
authors.initials: Z
authors.lastname: Zhong
keywords: Portulaca oleracea L; brain; high mobility group box 1; ischemia/reperfusion