title: DiAna, an ImageJ tool for object-based 3D co-localization and distance analysis.
abstract: We present a new plugin for ImageJ called DiAna, for Distance Analysis, which comes with a user-friendly interface. DiAna proposes robust and accurate 3D segmentation for object extraction. The plugin performs automated object-based co-localization and distance analysis. DiAna offers an in-depth analysis of co-localization between objects and retrieves 3D measurements including co-localizing volumes and surfaces of contact. It also computes the distribution of distances between objects in 3D. With DiAna, we furthermore introduce an original method, which allows for estimating the statistical significance of object co-localization. DiAna offers a complete and intuitive 3D image analysis tool for biologists.
authors.affiliation: Sorbonne Universités, UPMC Univ Paris 06, CNRS, Core Facility - Institut de Biologie Paris Seine (IBPS), 75005 Paris, France.
authors.forename: Jean-François
authors.initials: JF
authors.lastname: Gilles
authors.affiliation: Sorbonne Universités, UPMC Univ Paris 06, INSERM, CNRS, Neurosciences Paris Seine - Institut de Biologie Paris Seine (NPS - IBPS), 75005 Paris, France.
authors.forename: Marc
authors.initials: M
authors.lastname: Dos Santos
authors.affiliation: Sorbonne Universités, UPMC Univ Paris 06, IPAL, CNRS, Singapore; Bioinformatics Institute, Agency for Science, Technology and Research (A*STAR), Singapore.
authors.forename: Thomas
authors.initials: T
authors.lastname: Boudier
authors.affiliation: Sorbonne Universités, UPMC Univ Paris 06, CNRS, Core Facility - Institut de Biologie Paris Seine (IBPS), 75005 Paris, France.
authors.forename: Susanne
authors.initials: S
authors.lastname: Bolte
authors.affiliation: Sorbonne Universités, UPMC Univ Paris 06, INSERM, CNRS, Neurosciences Paris Seine - Institut de Biologie Paris Seine (NPS - IBPS), 75005 Paris, France. Electronic address: nicolas.heck@upmc.fr.
authors.forename: Nicolas
authors.initials: N
authors.lastname: Heck
mesh_terms: D000465:Algorithms; D000818:Animals; D000906:Antibodies / Q000737:chemistry; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D001921:Brain / Q000378:metabolism / Q000648:ultrastructure*; D005456:Fluorescent Dyes / Q000737:chemistry; D015870:Gene Expression; D055879:Gene Knock-In Techniques; D007091:Image Processing, Computer-Assisted / Q000379:methods* / Q000706:statistics & numerical data; D008164:Luminescent Proteins / Q000235:genetics / Q000378:metabolism; D008297:Male; D051379:Mice; D008822:Mice, Transgenic; D018613:Microscopy, Confocal / Q000295:instrumentation / Q000379:methods*; D008856:Microscopy, Fluorescence / Q000295:instrumentation / Q000379:methods*; D008867:Microtomy; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D012984:Software; D016708:Synaptophysin / Q000235:genetics / Q000378:metabolism; D014446:Tyrosine 3-Monooxygenase / Q000235:genetics / Q000378:metabolism; D050598:Vesicular Glutamate Transport Protein 1 / Q000235:genetics / Q000378:metabolism
chemical_list: D000906:Antibodies; D001426:Bacterial Proteins; C498441:Bsn protein, mouse; D005456:Fluorescent Dyes; D008164:Luminescent Proteins; D009419:Nerve Tissue Proteins; D011993:Recombinant Fusion Proteins; C493465:Slc17a7 protein, mouse; D016708:Synaptophysin; D050598:Vesicular Glutamate Transport Protein 1; C054562:yellow fluorescent protein, Bacteria; D014446:Tyrosine 3-Monooxygenase
keywords: 3D imaging; Deconvolution; Fluorescence microscopy; Image co-localization; Image segmentation; Spatial analysis