title: Effects of the Ayurved Siriraj Wattana recipe on functional and phenotypic characterization of cytokine-induced killer cells and dendritic cells in vitro.
abstract: BACKGROUND
  Ayurved Siriraj Wattana recipe (AVS073), has been prescribed as tonic, to increase appetite, and for pain relief. It also exhibits antioxidant, anti-inflammatory, immunomodulating and anti-cancer activities. However, the immunomodulatory effects on antigen-presenting cells and effector T cells remained elusive. We thus aimed to study the effects of AVS073 on differentiation, maturation, functions and proportions of CIK cells and monocyte-derived DCs.
  
  
  METHODS
  CIK cells and monocyte-derived DCs were treated with AVS073, followed by the assessment of T-helper (Th) phenotypes using real-time RT-PCR and flow cytometry.
  
  
  RESULTS
  AVS073 promoted Th1 phenotype in CD3+CD56+subset of CIK cells through increasing STAT4, T-bet, and interferon-γ. AVS073 inhibited Th2 phenotype through decreasing STAT6. AVS073 inhibited Treg phenotype through decreasing STAT5A, STAT5B and IDO. AVS073 promoted Th17 phenotype through increasing STAT3, RORC and IL-17. AVS073 treatment of mDCs resulted in increasing Th1-prone cytokine (IL-12) and Th17-prone cytokines (IL-6 and IL-23).
  
  
  CONCLUSIONS
  AVS073 upregulated Th1 and Th17, but downregulated Th2 and Treg phenotypes within CD3+CD56+cells. The treatment of mDCs drove Th1 and Th17-polarizations.
authors.affiliation: Department of Pharmacology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, 10700, Thailand.
authors.forename: Adisak
authors.initials: A
authors.lastname: Wongkajornsilp
authors.affiliation: Department of Pharmacology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, 10700, Thailand.
authors.forename: Nuntarak
authors.initials: N
authors.lastname: Numchaisermsuk
authors.affiliation: Department of Biochemistry, Faculty of Pharmacy, Mahidol University, Bangkok, 10400, Thailand.
authors.forename: Khanit
authors.initials: K
authors.lastname: Sa-Ngiamsuntorn
authors.affiliation: Department of Pharmacology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, 10700, Thailand.|Center of Applied Thai Traditional Medicine, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, 10700, Thailand.
authors.forename: Pravit
authors.initials: P
authors.lastname: Akarasereenont
authors.affiliation: Department of Pharmacology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, 10700, Thailand.
authors.forename: Valla
authors.initials: V
authors.lastname: Wamanuttajinda
authors.affiliation: Department of Pharmacology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, 10700, Thailand.
authors.forename: Kanda
authors.initials: K
authors.lastname: Kasetsinsombat
authors.affiliation: Department of Pharmacology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, 10700, Thailand.
authors.forename: Sunisa
authors.initials: S
authors.lastname: Duangsa-Ard
authors.affiliation: Center of Applied Thai Traditional Medicine, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, 10700, Thailand.
authors.forename: Tawee
authors.initials: T
authors.lastname: Laohapan
authors.affiliation: Department of Medicine, Faculty of Medicine Siriraj Hospital, Mahidol University, 2 Wanglang Road, Bangkoknoi, Bangkok, 10700, Thailand. kittipong.man@mahidol.ac.th.
authors.forename: Kittipong
authors.identifier: http://orcid.org/0000-0001-5940-7436
authors.initials: K
authors.lastname: Maneechotesuwan
mesh_terms: D017252:CD3 Complex; D019002:CD56 Antigen; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D055612:Cytokine-Induced Killer Cells / Q000187:drug effects*; D016207:Cytokines / Q000378:metabolism; D003713:Dendritic Cells / Q000187:drug effects*; D006801:Humans; D016130:Immunophenotyping; D028321:Plant Preparations / Q000494:pharmacology*; D010946:Plants, Medicinal / Q000737:chemistry*; D016176:T-Lymphocyte Subsets / Q000187:drug effects; D013785:Thailand
chemical_list: D017252:CD3 Complex; D019002:CD56 Antigen; D016207:Cytokines; D028321:Plant Preparations
keywords: CIK cells; Cytotoxicity; Dendritic cells; Immunomodulation; Lymphocytes; Traditional medicine Asia