title: Autoregulation of cardiac output is overcome by adrenergic stimulation in the anaconda heart.
abstract: Most vertebrates increase cardiac output during activity by elevating heart rate with relatively stable stroke volume. However, several studies have demonstrated 'intrinsic autoregulation' of cardiac output where artificially increased heart rate is associated with decreased stroke volume, leaving cardiac output unchanged. We explored the capacity of noradrenaline to overcome autoregulation in the anaconda heart. Electrically pacing in situ perfused hearts from the intrinsic heart rate to the maximum attainable resulted in a proportional decrease in stroke volume. However, noradrenaline, which increased heart rate to the same frequency as pacing, maintained stroke volume and thus increased cardiac output. In atrial and ventricular preparations, noradrenaline significantly increased the force of contraction and contraction kinetics. Thus, the increased contractility associated with adrenergic stimulation ameliorates filling limitations at high heart rates. Although heart rate appears the primary regulated variable during activity, this may only be achieved with compensatory amendments in myocardial contractility provided by adrenergic stimulation.
authors.affiliation: Department of Zoophysiology, Aarhus University, Aarhus C 8000, Denmark william.joyce@bios.au.dk.
authors.forename: William
authors.identifier: http://orcid.org/0000-0002-3782-1641
authors.initials: W
authors.lastname: Joyce
authors.affiliation: Department of Biological and Environmental Sciences, University of Gothenburg, Gothenburg 405 30, Sweden.
authors.forename: Michael
authors.initials: M
authors.lastname: Axelsson
authors.affiliation: Department of Zoophysiology, Aarhus University, Aarhus C 8000, Denmark.
authors.forename: Tobias
authors.initials: T
authors.lastname: Wang
mesh_terms: D000316:Adrenergic alpha-Agonists / Q000378:metabolism; D000818:Animals; D017821:Boidae / Q000502:physiology*; D002302:Cardiac Output; D006321:Heart / Q000502:physiology*; D006706:Homeostasis; D008297:Male; D009200:Myocardial Contraction; D009638:Norepinephrine / Q000378:metabolism*; D013318:Stroke Volume
chemical_list: D000316:Adrenergic alpha-Agonists; D009638:Norepinephrine
keywords: Cardiovascular; Heart rate; Noradrenaline; Stroke volume