title: Globularia alypum methanolic extract improves burn wound healing process and inflammation in rats and possesses antibacterial and antioxidant activities.
abstract: Burns are known as one of the most common and destructive forms of injury with a vast spectrum of consequences. Despite the discovery of various antibacterial and antiseptic agents, burn wound healing still has remained a challenge to modern medicine. Plants have been considered as potential agents for prevention and treatment of disorders in recent years. Globularia alypum L. (GA) is widely used in folk medicine against skin diseases and abscesses, however there is no scientific evidence justifying its use. This study aimed to evaluate the wound healing and anti-inflammatory effect, the antibacterial and antioxidant activities, as well as the chemical compositions of GA methanolic extract (GAME). Chemical compounds of GAME were examined by GC-MS. Wound healing effect was assessed by second-degree burn wounds in rats, anti-inflammatory activity was studied by carrageenan-induced rat paw edema, antioxidant activity was estimated by the DPPH, reducing power and β-carotene tests and antimicrobial activity was tested against 6 bacteria. A total of 17 compounds were identified. GAME-treated rats showed an improvement in healing process and carrageenan-induced hind paws edema as assessed by histological and biochemical investigations, compared to the control. A significant antioxidant and antibacterial activities were also observed in GAME-treated rats. GAME revealed a burn wound healing activity probably due to the anti-inflammatory, antimicrobial and antioxidant activities of its phytochemical contents. Thus, this study confirms its traditional use, however further more precise studies are needed for future clinical application.
authors.affiliation: Research Unit of Pharmacology and Toxicology of Xenobiotics, Faculty of Medicine, University of Sfax, 3029, Sfax, Tunisie. Electronic address: ghlissi_zohra@yahoo.fr.
authors.forename: Zohra
authors.initials: Z
authors.lastname: Ghlissi
authors.affiliation: Anatomopathology Laboratory, Habib Bourguiba University Hospital, 3029, Sfax, Tunisie.
authors.forename: Rim
authors.initials: R
authors.lastname: Kallel
authors.affiliation: Unit Enzymes & Bioconversion, National School of Engineers of Sfax, University of Sfax, 3038, Sfax, Tunisie.
authors.forename: Assaad
authors.initials: A
authors.lastname: Sila
authors.affiliation: Research Unit of Pharmacology and Toxicology of Xenobiotics, Faculty of Medicine, University of Sfax, 3029, Sfax, Tunisie.
authors.forename: Bahira
authors.initials: B
authors.lastname: Harrabi
authors.affiliation: Research Unit of Pharmacology and Toxicology of Xenobiotics, Faculty of Medicine, University of Sfax, 3029, Sfax, Tunisie.
authors.forename: Rim
authors.initials: R
authors.lastname: Atheymen
authors.affiliation: Research Unit of Pharmacology and Toxicology of Xenobiotics, Faculty of Medicine, University of Sfax, 3029, Sfax, Tunisie.
authors.forename: Khaled
authors.initials: K
authors.lastname: Zeghal
authors.affiliation: Unit Enzymes & Bioconversion, National School of Engineers of Sfax, University of Sfax, 3038, Sfax, Tunisie.
authors.forename: Ali
authors.initials: A
authors.lastname: Bougatef
authors.affiliation: Research Unit of Pharmacology and Toxicology of Xenobiotics, Faculty of Medicine, University of Sfax, 3029, Sfax, Tunisie.
authors.forename: Zouheir
authors.initials: Z
authors.lastname: Sahnoun
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000302:isolation & purification / Q000494:pharmacology* / Q000627:therapeutic use; D000893:Anti-Inflammatory Agents / Q000302:isolation & purification / Q000494:pharmacology* / Q000627:therapeutic use; D000975:Antioxidants / Q000302:isolation & purification / Q000494:pharmacology* / Q000627:therapeutic use; D007249:Inflammation / Q000188:drug therapy / Q000378:metabolism; D008297:Male; D000432:Methanol / Q000494:pharmacology / Q000627:therapeutic use; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology* / Q000627:therapeutic use; D018515:Plant Leaves; D000068377:Plantaginaceae; D051381:Rats; D017208:Rats, Wistar; D014945:Wound Healing / Q000187:drug effects* / Q000502:physiology
chemical_list: D000900:Anti-Bacterial Agents; D000893:Anti-Inflammatory Agents; D000975:Antioxidants; D010936:Plant Extracts; D000432:Methanol
keywords: Anti-inflammatory; GC–MS; Globularia alypum; Polyphenols; Wound healing