title: Laser and LED phototherapy on midpalatal suture after rapid maxilla expansion: Raman and histological analysis.
abstract: The aim of this study was to analyze the effect of laser or LED phototherapy on the acceleration of bone formation at the midpalatal suture after rapid maxilla expansion. Forty-five rats were divided into groups at 7 days (control, expansion, expansion and laser irradiation, and expansion and LED irradiation) and into 14 days (expansion, expansion and laser in the 1st week, expansion and LED in the 1st week, expansion and laser in the 1st and 2nd weeks, expansion and LED in the 1st and 2nd weeks). Laser/LED irradiation occurred every 48 h. Expansion was accomplished with a spatula and maintained with a triple helicoid of 0.020-in stainless steel orthodontic wire. A diode laser (λ780 nm, 70 mW, spot of 0.04 cm2, t = 257 s, SAEF of 18 J/cm2) or a LED (λ850 ± 10 nm, 150 ± 10 mW, spot of 0.5 cm2, t = 120 s, SAEF of 18 J/cm2) was applied in one point in the midpalatal suture immediately behind the upper incisors. Raman spectroscopy and histological analyses of the suture region were carried and data was submitted to statistical analyses (p ≤ 0.05). Raman spectrum analysis demonstrated that irradiation increases hydroxyapatite in the midpalatal suture after expansion. In the histological analysis of various inflammation, there was a higher production of collagen and osteoblastic activity and less osteoclastic activity. The results showed that LED irradiation associated to rapid maxillary expansion improves bone repair and could be an alternative to the use of laser in accelerating bone formation in the midpalatal suture.
authors.affiliation: Center of Biophotonics, School of Dentistry, Federal University of Bahia, Av. Araújo Pinho, 62, Canela, Salvador, BA, CEP 40110-150, Brazil.
authors.forename: Cristiane Becher
authors.initials: CB
authors.lastname: Rosa
authors.affiliation: Center of Biophotonics, School of Dentistry, Federal University of Bahia, Av. Araújo Pinho, 62, Canela, Salvador, BA, CEP 40110-150, Brazil.
authors.forename: Fernando Antonio Lima
authors.initials: FA
authors.lastname: Habib
authors.affiliation: Orthodontics, School of Dentistry, Federal University of Bahia, Av. Araújo Pinho, 62, Canela, Salvador, BA, CEP 40110-150, Brazil.
authors.forename: Telma Martins
authors.initials: TM
authors.lastname: de Araújo
authors.affiliation: Center of Biophotonics, School of Dentistry, Federal University of Bahia, Av. Araújo Pinho, 62, Canela, Salvador, BA, CEP 40110-150, Brazil.
authors.forename: Jean Nunes
authors.initials: JN
authors.lastname: Dos Santos
authors.affiliation: Center of Biophotonics, School of Dentistry, Federal University of Bahia, Av. Araújo Pinho, 62, Canela, Salvador, BA, CEP 40110-150, Brazil.
authors.forename: Maria Cristina T
authors.initials: MC
authors.lastname: Cangussu
authors.affiliation: Center of Biophotonics, School of Dentistry, Federal University of Bahia, Av. Araújo Pinho, 62, Canela, Salvador, BA, CEP 40110-150, Brazil.
authors.forename: Artur Felipe Santos
authors.initials: AF
authors.lastname: Barbosa
authors.affiliation: Center of Biophotonics, School of Dentistry, Federal University of Bahia, Av. Araújo Pinho, 62, Canela, Salvador, BA, CEP 40110-150, Brazil.
authors.forename: Isabele Cardoso Vieira
authors.initials: IC
authors.lastname: de Castro
authors.affiliation: Center of Biophotonics, School of Dentistry, Federal University of Bahia, Av. Araújo Pinho, 62, Canela, Salvador, BA, CEP 40110-150, Brazil. albp@ufba.br.|National Institute of Optics and Photonics, Physics Institute of São Carlos, University of São Paulo, São Carlos, SP, 13560-970, Brazil. albp@ufba.br.
authors.forename: Antônio Luiz Barbosa
authors.initials: AL
authors.lastname: Pinheiro
mesh_terms: D000818:Animals; D003094:Collagen / Q000378:metabolism; D007249:Inflammation / Q000473:pathology; D054023:Lasers, Semiconductor / Q000627:therapeutic use*; D008297:Male; D008437:Maxilla / Q000473:pathology* / Q000528:radiation effects*; D010155:Palatal Expansion Technique; D010159:Palate / Q000528:radiation effects*; D010789:Phototherapy / Q000379:methods*; D017208:Rats, Wistar; D013059:Spectrum Analysis, Raman; D013537:Sutures
chemical_list: D003094:Collagen
keywords: LED; Lasers; Orthodontics; Osteogenesis; Palatal expansion technique; Phototherapy