title: BoHV-4-based vector delivering Ebola virus surface glycoprotein.
abstract: Ebola virus (EBOV) is a Category A pathogen that is a member of Filoviridae family that causes hemorrhagic fever in humans and non-human primates. Unpredictable and devastating outbreaks of disease have recently occurred in Africa and current immunoprophylaxis and therapies are limited. The main limitation of working with pathogens like EBOV is the need for costly containment. To potentiate further and wider opportunity for EBOV prophylactics and therapies development, innovative approaches are necessary.
  
  
  
  In the present study, an antigen delivery platform based on a recombinant bovine herpesvirus 4 (BoHV-4), delivering a synthetic EBOV glycoprotein (GP) gene sequence, BoHV-4-syEBOVgD106ΔTK, was generated.
  
  
  
  EBOV GP was abundantly expressed by BoHV-4-syEBOVgD106ΔTK transduced cells without decreasing viral replication. BoHV-4-syEBOVgD106ΔTK immunized goats produced high titers of anti-EBOV GP antibodies and conferred a long lasting (up to 6 months), detectable antibody response. Furthermore, no evidence of BoHV-4-syEBOVgD106ΔTK viremia and secondary localization was detected in any of the immunized animals.
  
  
  
  The BoHV-4-based vector approach described here, represents: an alternative antigen delivery system for vaccination and a proof of principle study for anti-EBOV antibodies generation in goats for potential immunotherapy applications.
authors.affiliation: Department of Medical-Veterinary Science, University of Parma, Via del Taglio 10, 43126, Parma, Italy.
authors.forename: Alfonso
authors.initials: A
authors.lastname: Rosamilia
authors.affiliation: Department of Medical-Veterinary Science, University of Parma, Via del Taglio 10, 43126, Parma, Italy.
authors.forename: Sarah
authors.initials: S
authors.lastname: Jacca
authors.affiliation: Department of Medical-Veterinary Science, University of Parma, Via del Taglio 10, 43126, Parma, Italy.
authors.forename: Giulia
authors.initials: G
authors.lastname: Tebaldi
authors.affiliation: Department of Medical-Veterinary Science, University of Parma, Via del Taglio 10, 43126, Parma, Italy.
authors.forename: Silvia
authors.initials: S
authors.lastname: Tiberti
authors.affiliation: Department of Medical-Veterinary Science, University of Parma, Via del Taglio 10, 43126, Parma, Italy.
authors.forename: Valentina
authors.initials: V
authors.lastname: Franceschi
authors.affiliation: Department of Medical-Veterinary Science, University of Parma, Via del Taglio 10, 43126, Parma, Italy.
authors.forename: Francesca
authors.initials: F
authors.lastname: Macchi
authors.affiliation: Department of Medical-Veterinary Science, University of Parma, Via del Taglio 10, 43126, Parma, Italy.
authors.forename: Sandro
authors.initials: S
authors.lastname: Cavirani
authors.affiliation: Special Pathogens Program, University of Manitoba and Public Health Agency of Canada, Winnipeg, MB, Canada.
authors.forename: Gary
authors.initials: G
authors.lastname: Kobinger
authors.affiliation: Animal Disease Research Unit, Agricultural Research Service, United States Department of Agriculture, and Department of Veterinary Microbiology & Pathology, Washington State University, Pullman, WA, USA.
authors.forename: Donald P
authors.initials: DP
authors.lastname: Knowles
authors.affiliation: Department of Medical-Veterinary Science, University of Parma, Via del Taglio 10, 43126, Parma, Italy. gaetano.donofrio@unipr.it.
authors.forename: Gaetano
authors.identifier: 0000-0002-8984-0377
authors.initials: G
authors.lastname: Donofrio
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002417:Cattle; D002460:Cell Line; D003062:Codon / Q000235:genetics; D003198:Computer Simulation; D029043:Ebolavirus / Q000378:metabolism*; D005822:Genetic Vectors / Q000378:metabolism*; D006041:Goats / Q000276:immunology; D057809:HEK293 Cells; D026021:Herpesvirus 4, Bovine / Q000378:metabolism*; D006801:Humans; D056724:Immunity, Humoral; D007114:Immunization; D007700:Kinetics; D008562:Membrane Glycoproteins / Q000737:chemistry / Q000378:metabolism*; D016366:Open Reading Frames / Q000235:genetics
chemical_list: D003062:Codon; D008562:Membrane Glycoproteins
keywords: Bovine herpesvirus 4; Ebola virus; Recombineering; Vaccine platform; Viral vector