title: Extended-focus optical coherence microscopy for high-resolution imaging of the murine brain.
abstract: We propose a new method and optical instrumentation for mouse brain imaging based on extended-focus optical coherence microscopy. This in vivo imaging technique allows the evaluation of the cytoarchitecture at cellular level and the circulation system dynamics in three dimensions. This minimally invasive and non-contact approach is performed without the application of contrasting agents. The optical design achieved a resolution of 2.2 μm over a distance of 800 μm, which was sufficient to obtain a detailed three-dimensional image of a wild-type mouse's brain down to the layer III of the cortex. Intrinsically contrasted microvessels and structures similar to the bodies of neurons were distinguishable.
authors.affiliation: Institute of Physics, Faculty of Physics, Astronomy and Informatics, Nicolaus Copernicus University Grudziadzka 5, 87-100 Torun, Poland.
authors.forename: Szymon
authors.initials: S
authors.lastname: Tamborski
authors.affiliation: Institute of Physics, Faculty of Physics, Astronomy and Informatics, Nicolaus Copernicus University Grudziadzka 5, 87-100 Torun, Poland.
authors.forename: Hong Chou
authors.initials: HC
authors.lastname: Lyu
authors.affiliation: Nencki Institute of Experimental Biology, 3 Pasteur Street, 02-093 Warsaw, Poland.
authors.forename: Hubert
authors.initials: H
authors.lastname: Dolezyczek
authors.affiliation: Nencki Institute of Experimental Biology, 3 Pasteur Street, 02-093 Warsaw, Poland.
authors.forename: Monika
authors.initials: M
authors.lastname: Malinowska
authors.affiliation: Nencki Institute of Experimental Biology, 3 Pasteur Street, 02-093 Warsaw, Poland.
authors.forename: Grzegorz
authors.initials: G
authors.lastname: Wilczynski
authors.affiliation: Laboratoire d'Optique Biomédicale, École Polytechnique Fédérale de Lausanne, CH-1015 Lausanne, Switzerland.
authors.forename: Daniel
authors.initials: D
authors.lastname: Szlag
authors.affiliation: Laboratoire d'Optique Biomédicale, École Polytechnique Fédérale de Lausanne, CH-1015 Lausanne, Switzerland.
authors.forename: Theo
authors.initials: T
authors.lastname: Lasser
authors.affiliation: Institute of Physics, Faculty of Physics, Astronomy and Informatics, Nicolaus Copernicus University Grudziadzka 5, 87-100 Torun, Poland.
authors.forename: Maciej
authors.initials: M
authors.lastname: Wojtkowski
authors.affiliation: Institute of Physics, Faculty of Physics, Astronomy and Informatics, Nicolaus Copernicus University Grudziadzka 5, 87-100 Torun, Poland.
authors.forename: Maciej
authors.initials: M
authors.lastname: Szkulmowski
keywords: (110.2945) Illumination design; (110.3175) Interferometric imaging; (110.4500) Optical coherence tomography; (170.3880) Medical and biological imaging