title: Isolation and Characterisation of Bacteriocin and Aggregation-Promoting Factor Production inLactococcus lactisssp.lactisBGBM50 Strain.
abstract: Lactococcus lactisssp.lactisBGBM50, a producer of lactococcin G and aggregation-promoting factor, was isolated from selected lactic acid bacteria taken from semi-hard cheese traditionally produced in the village Žanjic, Montenegro. Strain BGBM50 harbours a number of plasmids of different sizes. Plasmid curing experiments showed that genes for bacteriocin production are located on pBM140, a plasmid 140 kb in length. PCR analysis with primers specific for lactococcin Q and G genes gave fragment of the expected size. In addition, after plasmid curing of strain BGBM50, different derivatives with altered phenotypes were obtained, among them BGBM50-34 strain, which retained bacteriocin synthesis but had enhanced aggregation ability.
authors.affiliation: Faculty of Agriculture, University of Belgrade, RS-11080 Belgrade, Serbia; Institute of Molecular Genetics and Genetic Engineering, University of Belgrade, RS-11010 Belgrade, Serbia.
authors.forename: Nemanja
authors.initials: N
authors.lastname: Mirkovic
authors.affiliation: Faculty of Agriculture, University of Belgrade, RS-11080 Belgrade, Serbia.
authors.forename: Zorica
authors.initials: Z
authors.lastname: Radulovic
authors.affiliation: Institute of Molecular Genetics and Genetic Engineering, University of Belgrade, RS-11010 Belgrade, Serbia.
authors.forename: Gordana
authors.initials: G
authors.lastname: Uzelac
authors.affiliation: Institute of Molecular Genetics and Genetic Engineering, University of Belgrade, RS-11010 Belgrade, Serbia; Faculty of Biology, University of Belgrade, RS-11000 Belgrade, Serbia.
authors.forename: Jelena
authors.initials: J
authors.lastname: Lozo
authors.affiliation: Faculty of Agriculture, University of Belgrade, RS-11080 Belgrade, Serbia.
authors.forename: Dragojlo
authors.initials: D
authors.lastname: Obradovic
authors.affiliation: Institute of Molecular Genetics and Genetic Engineering, University of Belgrade, RS-11010 Belgrade, Serbia.
authors.forename: Ljubisa
authors.initials: L
authors.lastname: Topisirovic
authors.affiliation: Institute of Molecular Genetics and Genetic Engineering, University of Belgrade, RS-11010 Belgrade, Serbia.
authors.forename: Milan
authors.initials: M
authors.lastname: Kojic
keywords: aggregation-promoting factor; bacteriocin lactococcin G; plasmid curing