title: Simultaneous determination and pharmacokinetic study of four phenolic acids in rat plasma using UFLC-MS/MS after intravenous administration of salvianolic acid for injection.
abstract: A simple, sensitive and selective ultra-fast liquid chromatography-tandem mass spectrometry (UFLC-MS/MS) method was established for simultaneous determination and pharmacokinetic study of rosmarinic acid (RA), salvianolic acid D (Sal D), lithospermic acid (LA) and salvianolic acid B (Sal B) in rat plasma after intravenous administration of salvianolic acid for injection (SAFI). Three doses of administration, containing 14, 28 and 56mg/kg, were investigated in this study. Plasma samples were pretreated using protein precipitation (PP) with pre-cooled acetonitrile. Chromatographic separation was achieved on a CORTECS™ UPLC C18 column (1.6μm, 2.1×100mm) with a mobile phase composed of 0.1% formic acid aqueous (V/V) and 0.1% formic acid acetonitrile (V/V). Analytes were detected using electrospray ionization (ESI) source in negative ionization mode and quantified in multiple reaction monitoring (MRM) mode. The validated method is stable and reliable. No significant difference of half lives (t1/2) of four analytes at three doses was observed. Area under the curve (AUC0-∞) and peak concentration (Cmax) of the four analytes demonstrated a linear increase in across the doses with the linear correlation r of each analyte at three doses were greater than 0.95. It indicated that the pharmacokinetic behavior of SAFI is positively related to dose at the range of 14-56mg/kg.
authors.affiliation: School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, People's Republic of China.
authors.forename: Xiuman
authors.initials: X
authors.lastname: Xie
authors.affiliation: School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, People's Republic of China.
authors.forename: Jingzhuo
authors.initials: J
authors.lastname: Miao
authors.affiliation: School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, People's Republic of China.
authors.forename: Wanyang
authors.initials: W
authors.lastname: Sun
authors.affiliation: Tasly Academy, State Key Laboratory of Core Technology in Innovative Chinese Medicine, Tasly Pharmaceutical Analysis Institute, Tianjin 300402, People's Republic of China.
authors.forename: Jingyi
authors.initials: J
authors.lastname: Huang
authors.affiliation: Tasly Academy, State Key Laboratory of Core Technology in Innovative Chinese Medicine, Tasly Pharmaceutical Analysis Institute, Tianjin 300402, People's Republic of China.
authors.forename: Dongxiang
authors.initials: D
authors.lastname: Li
authors.affiliation: Tasly Academy, State Key Laboratory of Core Technology in Innovative Chinese Medicine, Tasly Pharmaceutical Analysis Institute, Tianjin 300402, People's Republic of China.
authors.forename: Shuming
authors.initials: S
authors.lastname: Li
authors.affiliation: Tasly Academy, State Key Laboratory of Core Technology in Innovative Chinese Medicine, Tasly Pharmaceutical Analysis Institute, Tianjin 300402, People's Republic of China. Electronic address: tongling@tasly.com.
authors.forename: Ling
authors.initials: L
authors.lastname: Tong
authors.affiliation: School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, People's Republic of China. Electronic address: gxswmwys@163.com.
authors.forename: Guoxiang
authors.initials: G
authors.lastname: Sun
mesh_terms: D061605:Administration, Intravenous; D000475:Alkenes / Q000008:administration & dosage* / Q000097:blood*; D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage* / Q000493:pharmacokinetics*; D062385:Hydroxybenzoates / Q000097:blood*; D008297:Male; D059808:Polyphenols / Q000008:administration & dosage* / Q000097:blood*; D051381:Rats; D017208:Rats, Wistar; D027561:Salvia miltiorrhiza; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D000475:Alkenes; D004365:Drugs, Chinese Herbal; D062385:Hydroxybenzoates; D059808:Polyphenols; C568740:salvianolic acid; C017616:phenolic acid
keywords: Pharmacokinetics; Protein precipitation; Salvianolic acid for injection; Ultra-fast liquid chromatography-tandem mass spectrometry