title: Cryopreservation of adult cervid testes.
abstract: Several species of cervids are currently classified as threatened or endangered due to a rapid decline in their populations. Sperm cryopreservation, in association with assisted reproductive technologies, can find application for the conservation of endangered cervids. In cases of unsuccessful sperm retrieval through other means prior to the death of the animal, adult testis is the only source of sperm. Recovery of viable sperm from adult testes depends on the effective preservation of testicular tissues through optimization of cryopreservation protocols. The present study evaluated combinations of 10% dimethyl sulfoxide (DMSO) with 0% or 80% fetal bovine serum (FBS) and 20% DMSO with 0 or 20% FBS for the cryopreservation of testicular tissues of three adult cervids using uncontrolled slow freezing protocol. The cryopreserved testis was compared to chilled tissue without cryoprotectants. Results revealed that testicular tissues of barking deer cryopreserved in 20% DMSO (D20) had all the analyzed 7 parameters (number of TNP1-, PRM2 and acrosin-expressing cells/tubule and, the number of viable, morphologically normal, acrosome intact, Annexin V-negative sperm) comparable to the chilled testis. However, testicular tissues of sambhar and hog deer cryopreserved only in D20S20 had 5 of 7 parameters comparable to the chilled testis. In conclusion, D20 is acceptable for cryopreservation of barking deer and D20S20 for sambar and hog deer testes.
authors.affiliation: Laboratory for the Conservation of Endangered Species, Centre for Cellular and Molecular Biology, Council for Scientific and Industrial Research, Uppal Road, Hyderabad, 500 007, India.
authors.forename: Lavanya
authors.initials: L
authors.lastname: Pothana
authors.affiliation: Laboratory for the Conservation of Endangered Species, Centre for Cellular and Molecular Biology, Council for Scientific and Industrial Research, Uppal Road, Hyderabad, 500 007, India.
authors.forename: Lalitha
authors.initials: L
authors.lastname: Devi
authors.affiliation: Laboratory for the Conservation of Endangered Species, Centre for Cellular and Molecular Biology, Council for Scientific and Industrial Research, Uppal Road, Hyderabad, 500 007, India. Electronic address: sandeep@ccmb.res.in.
authors.forename: Sandeep
authors.initials: S
authors.lastname: Goel
mesh_terms: D000176:Acrosin / Q000378:metabolism; D000177:Acrosome / Q000502:physiology; D000818:Animals; D002868:Chromosomal Proteins, Non-Histone / Q000378:metabolism; D015925:Cryopreservation / Q000379:methods / Q000662:veterinary*; D003451:Cryoprotective Agents / Q000494:pharmacology*; D003670:Deer; D004121:Dimethyl Sulfoxide / Q000494:pharmacology*; D008297:Male; D011479:Protamines / Q000378:metabolism; D012662:Semen Preservation / Q000379:methods / Q000662:veterinary*; D013737:Testis / Q000502:physiology*
chemical_list: D002868:Chromosomal Proteins, Non-Histone; D003451:Cryoprotective Agents; D011479:Protamines; C068621:protamine 2; C053886:spermatid transition proteins; D000176:Acrosin; D004121:Dimethyl Sulfoxide
keywords: Cryopreservation; DMSO; Deer; FBS; Slow freezing; Testis