title: Inheritance and effectiveness of two transgenes determining PVY resistance in progeny from crossing independently transformed tobacco lines.
abstract: Genetic transformation of plants allows us to obtain improved genotypes enriched with the desired traits. However, if transgenic lines were to be used in breeding programs the stability of inserted transgenes is essential. In the present study, we followed the inheritance of transgenes in hybrids originated from crossing two transgenic tobacco lines resistant to Potato virus Y (PVY): MN 944 LMV with the transgene containing Lettuce mosaic virus coat protein gene (LMV CP) and AC Gayed ROKY2 with PVY replicase gene (ROKY2). Progeny populations generated by successive self-pollination were analyzed with respect to the transgene segregation ratio and resistance to Potato virus Y in tests carried out under greenhouse conditions. The presence of the virus in inoculated plants was detected by DAS-ELISA method. The results demonstrated the Mendelian fashion of inheritance of transgenes which were segregated independently and stably. As a result, we obtained T4generation of hybrid with both transgenes stacked and which was highly resistant to PVY.
authors.affiliation: Department of Plant Breeding and Biotechnology, Institute of Soil Science and Plant Cultivation - State Research Institute, 8 Czartoryskich Street, 24-100, Puławy, Poland. annacz@iung.pulawy.pl.
authors.forename: Anna
authors.initials: A
authors.lastname: Czubacka
authors.affiliation: Council for Agricultural Research and Economics-Ornamental Plant Research Unit, (CREA-FSO), 508, Corso degli Inglesi, 18038, Sanremo, IM, Italy.
authors.forename: Ermanno
authors.initials: E
authors.lastname: Sacco
authors.affiliation: Department of Plant Breeding and Biotechnology, Institute of Soil Science and Plant Cultivation - State Research Institute, 8 Czartoryskich Street, 24-100, Puławy, Poland.
authors.forename: Hanna
authors.initials: H
authors.lastname: Olszak-Przybyś
authors.affiliation: Department of Plant Breeding and Biotechnology, Institute of Soil Science and Plant Cultivation - State Research Institute, 8 Czartoryskich Street, 24-100, Puławy, Poland.
authors.forename: Teresa
authors.initials: T
authors.lastname: Doroszewska
mesh_terms: D003433:Crosses, Genetic; D060467:Disease Resistance / Q000235:genetics*; D040582:Inheritance Patterns; D010641:Phenotype; D010935:Plant Diseases / Q000235:genetics* / Q000821:virology; D030821:Plants, Genetically Modified / Q000235:genetics / Q000821:virology; D017800:Potyvirus; D014026:Nicotiana / Q000235:genetics* / Q000821:virology; D014170:Transformation, Genetic; D019076:Transgenes
keywords: Resistance to Potato virus Y; Tobacco; Transgene inheritance; Transgenic hybrids