title: Elevated levels of p-Mnk1, p-eIF4E and p-p70S6K proteins are associated with tumor recurrence and poor prognosis in astrocytomas.
abstract: Malignant astrocytomas are able to invade neighboring and distant areas of the normal brain. Signaling pathway alterations play important role in the development of astrocytomas. Deregulation of eukaryotic translation initiation factor 4E (eIF4E) by MAP kinase-interacting kinases (Mnk) on Ser-209 directly or PI3K/mTOR/S6K pathway indirectly has a critical effect on promoting cellular proliferation, malignant transformation and metastasis. We examined and analyzed the correlation between expression of p-Mnk1, p-eIF4E and p-p70S6K proteins and clinicopathological features in 103 astrocytomas and 54 non-tumorous brain tissues. The results indicated that positive percentage of overexpression of p-Mnk1 and p-eIF4E proteins in astrocytomas were significantly higher than that of in the non-tumorous brain tissues (P < 0.05). Elevated p-Mnk1 and p-eIF4E and co-overexpressed three proteins were associated with tumor recurrence (P = 0.003, P = 0.006, P = 0.007, respectively). Overexpressed p-eIF4E significantly correlated with the tumor size (P = 0.019). In addition, overexpression of p-eIF4E and three proteins common expression were related to the WHO grade of astrocytomas (P = 0.001, P = 0.044 respectively). Spearman's rank correlation test further showed that the expression of p-Mnk1 was strongly positive correlated with the expression of p-eIF4E in astrocytomas (r = 0.294, P = 0.003). Besides, overexpression of p-eIF4E and co-expression of p-Mnk1, p-eIF4E and p-p70S6K proteins were inversely correlated with overall survival rates of astrocytomas. Multivariate Cox regression analysis further identified that the elevated p-eIF4E expression, three proteins common expression were correlated with unfavorable prognosis of astrocytomas regardless of ages and WHO grades. Taken together, overexpression of p-eIF4E and co-expression of p-Mnk1, p-eIF4E and p-p70S6K proteins could be used as novel independent poor prognostic biomarkers for patients with astrocytomas.
authors.affiliation: Department of Neurology, The Third Hospital of Changsha, Changsha, 410011, Hunan, China.
authors.forename: Weibing
authors.initials: W
authors.lastname: Fan
authors.affiliation: Department of Pathology, The Second Xiangya Hospital of Central South University, Changsha, 410011, Hunan, China. wwyyww.123@163.com.
authors.forename: Weiyuan
authors.initials: W
authors.lastname: Wang
authors.affiliation: Department of Neurology, The Third Hospital of Changsha, Changsha, 410011, Hunan, China.
authors.forename: Xinfa
authors.initials: X
authors.lastname: Mao
authors.affiliation: Department of Pathology, The Second Xiangya Hospital of Central South University, Changsha, 410011, Hunan, China.
authors.forename: Shuzhou
authors.initials: S
authors.lastname: Chu
authors.affiliation: Department of Pathology, The Second Xiangya Hospital of Central South University, Changsha, 410011, Hunan, China.
authors.forename: Juan
authors.initials: J
authors.lastname: Feng
authors.affiliation: Department of Pathology, Xiangya School of Medicine, Central South University, Changsha, 410011, Hunan, China.
authors.forename: Desheng
authors.initials: D
authors.lastname: Xiao
authors.affiliation: Department of Pathology, Xiangya School of Medicine, Central South University, Changsha, 410011, Hunan, China.
authors.forename: Jianhua
authors.initials: J
authors.lastname: Zhou
authors.affiliation: Department of Pathology, The Second Xiangya Hospital of Central South University, Changsha, 410011, Hunan, China. fansongqing2003@hotmail.com.
authors.forename: Songqing
authors.initials: S
authors.lastname: Fan
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D001254:Astrocytoma / Q000175:diagnosis / Q000378:metabolism*; D014408:Biomarkers, Tumor / Q000378:metabolism; D001932:Brain Neoplasms / Q000175:diagnosis / Q000378:metabolism*; D002648:Child; D004268:DNA-Binding Proteins / Q000378:metabolism*; D005260:Female; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins / Q000378:metabolism*; D053208:Kaplan-Meier Estimate; D008297:Male; D008875:Middle Aged; D009364:Neoplasm Recurrence, Local / Q000175:diagnosis / Q000378:metabolism*; D010766:Phosphorylation; D011379:Prognosis; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism*; D038762:Ribosomal Protein S6 Kinases, 70-kDa / Q000378:metabolism*; D014157:Transcription Factors / Q000378:metabolism*; D055815:Young Adult
chemical_list: D014408:Biomarkers, Tumor; D004268:DNA-Binding Proteins; C111931:ELF4 protein, human; D047908:Intracellular Signaling Peptides and Proteins; D014157:Transcription Factors; C491395:MKNK1 protein, human; D017346:Protein Serine-Threonine Kinases; D038762:Ribosomal Protein S6 Kinases, 70-kDa
keywords: Astrocytomas; P-Mnk1; Prognosis; Recurrence; p-EIF4E