title: Zataria multifloraBioss: lethal effects of methanolic extract against protoscoleces ofEchinococcus granulosus.
abstract: The present study was aimed to evaluate the lethal effects ofZataria multifloraBoiss (Lamiaceae) methanolic extract againstEchinococcus granulosusprotoscoleces. Protoscoleces were aseptically aspirated from sheep livers having hydatid cysts. Various concentrations of the essential oil (2.5-20 mg/mL) were used for 10-60 min. Viability of protoscoleces was confirmed using eosin exclusion test (0.1 % eosin staining). Obtained results showed thatZ. multifloraextract at the concentration of 20 mg/mL after 10 min of exposure killed 100 % protoscoleces. The mean of mortality rate of protoscoleces after 20 min of exposure to the concentration of 10 mg/mL was also 100 %. Lower concentrations ofZ. multifloraextract provoked a delayed protoscolicidal activity. The findings indicated potential ofZ. multifloramethanolic extract as a natural source for the producing of new scolicidal agent for use in hydatid cyst surgery.
authors.affiliation: Herbal and Traditional Medicines Research Center, Kerman University of Medical Sciences, Kerman, Iran.
authors.forename: Sareh
authors.initials: S
authors.lastname: Jahanbakhsh
authors.affiliation: Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran.
authors.forename: Mojgan
authors.initials: M
authors.lastname: Azadpour
authors.affiliation: Department of Medical Parasitology, Iran University of Medical Sciences, Tehran, Iran.
authors.forename: Amir
authors.initials: A
authors.lastname: Tavakoli Kareshk
authors.affiliation: Department of Medical Parasitology, Jundishapour University of Medical Sciences, Ahvaz, Iran.
authors.forename: Amir
authors.initials: A
authors.lastname: Keyhani
authors.affiliation: Herbal and Traditional Medicines Research Center, Kerman University of Medical Sciences, Kerman, Iran.
authors.forename: Hossein
authors.initials: H
authors.lastname: Mahmoudvand
keywords: Cystic echinococcosis; Protoscoleces; Scolicidal; Zataria multiflora Boiss