title: Detection of autoantibodies to DSF70/LEDGFp75 in Mexican Hispanics using multiple complementary assay platforms.
abstract: OBJECTIVE
  Antinuclear autoantibodies (ANA) targeting the dense fine speckled antigen DFS70, also known as lens epithelium-derived growth factor p75 (LEDGF/p75), are attracting attention due to their low frequency in systemic rheumatic diseases but increased frequency in clinical laboratory referrals and healthy individuals (HI). These ANA specifically recognize the stress protein DFS70/LEDGFp75, implicated in cancer, HIV-AIDS, and inflammation. While their frequency has been investigated in various ethnic populations, there is little information on their frequency among Hispanics/Latinos. In this study, we determined the frequency of anti-DFS70/LEDGFp75 autoantibodies in Mexican Hispanics using multiple detection platforms.
  
  
  METHODS
  The frequency of anti-DFS70/LEDGFp75 antibodies was determined in 171 individuals, including 71 dermatomyositis (DM) patients, 47 rheumatoid arthritis (RA) patients, 30 obesity (OB) patients, and 23 HI. Antibody detection was achieved using four complementary assay platforms: indirect immunofluorescence, Western blotting, ELISA, and chemiluminescent immunoassay.
  
  
  RESULTS
  We detected relatively low frequencies of anti-DFS70/LEDGFp75 antibodies in patients with DM (1.4%), RA (4.3%), and OB (6.6%), and elevated frequency (17.4%) in HI. A strong concordance between the different antibody detection platforms was observed.
  
  
  CONCLUSIONS
  The low frequency of anti-DFS70/LEDGFp75 antibodies in Mexican patients with rheumatic diseases, but relatively higher frequency in HI, is consistent with previous observations with non-Hispanic populations, suggesting that geographic differences or ethnicity do not influence the frequency of these autoantibodies. Our results also highlight the importance of confirmatory assays for the accurate detection of these autoantibodies. Future studies with larger cohorts of healthy Hispanics/Latinos are needed to confirm if their anti-DFS70/LEDGFp75 antibody frequencies are significantly higher than in non-Hispanics.
authors.affiliation: Instituto de Investigación en Reumatología y del Sistema Músculo Esquelético, CUCS, Universidad de Guadalajara, Guadalajara, Jalisco, Mexico.|Servicio de Reumatología, Posgrado 004086 PNPC-CONACyT, División de Medicina Interna, O.P.D., Hospital Civil Dr. Juan I. Menchaca, Guadalajara, Jalisco, Mexico.
authors.forename: Mónica
authors.initials: M
authors.lastname: Vázquez-Del Mercado
authors.affiliation: Instituto de Investigación en Reumatología y del Sistema Músculo Esquelético, CUCS, Universidad de Guadalajara, Guadalajara, Jalisco, Mexico.
authors.forename: Eduardo
authors.initials: E
authors.lastname: Gómez-Bañuelos
authors.affiliation: Instituto de Investigación en Reumatología y del Sistema Músculo Esquelético, CUCS, Universidad de Guadalajara, Guadalajara, Jalisco, Mexico.
authors.forename: Rosa Elena
authors.initials: RE
authors.lastname: Navarro-Hernández
authors.affiliation: Instituto de Investigación en Reumatología y del Sistema Músculo Esquelético, CUCS, Universidad de Guadalajara, Guadalajara, Jalisco, Mexico.
authors.forename: Oscar
authors.initials: O
authors.lastname: Pizano-Martinez
authors.affiliation: Instituto de Investigación en Reumatología y del Sistema Músculo Esquelético, CUCS, Universidad de Guadalajara, Guadalajara, Jalisco, Mexico.
authors.forename: Adan
authors.initials: A
authors.lastname: Saldaña-Millán
authors.affiliation: Instituto de Investigación en Reumatología y del Sistema Músculo Esquelético, CUCS, Universidad de Guadalajara, Guadalajara, Jalisco, Mexico.
authors.forename: Efrain
authors.initials: E
authors.lastname: Chavarria-Avila
authors.affiliation: Servicio de Reumatología, Posgrado 004086 PNPC-CONACyT, División de Medicina Interna, O.P.D., Hospital Civil Dr. Juan I. Menchaca, Guadalajara, Jalisco, Mexico.
authors.forename: Lorena
authors.initials: L
authors.lastname: Gonzalez-Rosas
authors.affiliation: Servicio de Reumatología, Centro Médico Nacional 20 de Noviembre ISSSTE, Mexico, D.F., Mexico.
authors.forename: Lilia
authors.initials: L
authors.lastname: Andrade-Ortega
authors.affiliation: Hospital de Especialidades, Centro Médico Nacional La Raza, IMSS, Mexico, D.F., Mexico.
authors.forename: Miguel Angel
authors.initials: MA
authors.lastname: Saavedra
authors.affiliation: Hospital de Especialidades, Centro Médico Nacional La Raza, IMSS, Mexico, D.F., Mexico.
authors.forename: Olga Lidia
authors.initials: OL
authors.lastname: Vera-Lastra
authors.affiliation: Hospital de Especialidades, Centro Médico Nacional La Raza, IMSS, Mexico, D.F., Mexico.
authors.forename: Luis Javier
authors.initials: LJ
authors.lastname: Jara
authors.affiliation: Servicio de Reumatología, Hospital General de México, Mexico, D.F., Mexico.
authors.forename: Gabriel
authors.initials: G
authors.lastname: Medrano-Ramírez
authors.affiliation: Hospital de Especialidades, Centro Médico Nacional La Raza, IMSS, Mexico, D.F., Mexico.
authors.forename: Claudia
authors.initials: C
authors.lastname: Cruz-Reyes
authors.affiliation: Servicio de Inmunología y Reumatología, Hospital General de Occidente, Zapopan, Jalisco, Mexico.
authors.forename: Ignacio
authors.initials: I
authors.lastname: García-De la Torre
authors.affiliation: Instituto de Investigación en Reumatología y del Sistema Músculo Esquelético, CUCS, Universidad de Guadalajara, Guadalajara, Jalisco, Mexico.
authors.forename: Marta
authors.initials: M
authors.lastname: Escarra-Senmarti
authors.affiliation: Department of Basic Sciences, Center for Health Disparities and Molecular Medicine, Loma Linda University School of Medicine, Mortensen Hall 142, 11085 Campus St., Loma Linda, CA, 92350, USA.
authors.forename: Lisiane Maria Enriconi-Dos
authors.initials: LME
authors.lastname: Anjos
authors.affiliation: Department of Basic Sciences, Center for Health Disparities and Molecular Medicine, Loma Linda University School of Medicine, Mortensen Hall 142, 11085 Campus St., Loma Linda, CA, 92350, USA.
authors.forename: Anamika
authors.initials: A
authors.lastname: Basu
authors.affiliation: Department of Research, Inova Diagnostics, Inc., San Diego, CA, USA.
authors.forename: Roger
authors.initials: R
authors.lastname: Albesa
authors.affiliation: Department of Research, Inova Diagnostics, Inc., San Diego, CA, USA.
authors.forename: Michael
authors.initials: M
authors.lastname: Mahler
authors.affiliation: Department of Basic Sciences, Center for Health Disparities and Molecular Medicine, Loma Linda University School of Medicine, Mortensen Hall 142, 11085 Campus St., Loma Linda, CA, 92350, USA. ccasiano@llu.edu.|Department of Medicine, Division of Rheumatology, Loma Linda University School of Medicine, Loma Linda, CA, USA. ccasiano@llu.edu.
authors.forename: Carlos A
authors.initials: CA
authors.lastname: Casiano
keywords: Autoantibodies; DFS70; Frequency; Hispanics; LEDGF/p75; Mexican; Multiple assay platforms