title: Site-selective orientated immobilization of antibodies and conjugates for immunodiagnostics development.
abstract: Immobilized antibody systems are the key to develop efficient diagnostics and separations tools. In the last decade, developments in the field of biomolecular engineering and crosslinker chemistry have greatly influenced the development of this field. With all these new approaches at our disposal, several new immobilization methods have been created to address the main challenges associated with immobilized antibodies. Few of these challenges that we have discussed in this review are mainly associated to the site-specific immobilization, appropriate orientation, and activity retention. We have discussed the effect of antibody immobilization approaches on the parameters on the performance of an immunoassay.
authors.affiliation: Department of Chemistry, University of Connecticut, Storrs, Connecticut 06269-3060.
authors.forename: Min
authors.initials: M
authors.lastname: Shen
authors.affiliation: Department of Chemistry, University of Connecticut, Storrs, Connecticut 06269-3060.|Institute of Materials Science, University of Connecticut, Storrs, Connecticut 06269-3136.|Department of Cell Biology, University of Connecticut Health Center, Farmington, Connecticut 060.|School of Chemistry, National University of Ireland at Galway, Galway, Ireland.
authors.forename: James
authors.initials: J
authors.lastname: Rusling
authors.affiliation: Department of Chemistry, University of Connecticut, Storrs, Connecticut 06269-3060.
authors.forename: Chandra K
authors.initials: CK
authors.lastname: Dixit
mesh_terms: D000327:Adsorption; D055910:Antibodies, Immobilized / Q000737:chemistry*; D000941:Antigens / Q000737:chemistry / Q000276:immunology; D001665:Binding Sites; D015374:Biosensing Techniques; D057930:Click Chemistry / Q000379:methods*; D003432:Cross-Linking Reagents / Q000737:chemistry; D061565:Cycloaddition Reaction / Q000379:methods*; D006801:Humans; D007118:Immunoassay; D018796:Immunoconjugates / Q000737:chemistry*; D009841:Oligonucleotides / Q000737:chemistry; D011485:Protein Binding; D013205:Staphylococcal Protein A / Q000737:chemistry
chemical_list: D055910:Antibodies, Immobilized; D000941:Antigens; D003432:Cross-Linking Reagents; D018796:Immunoconjugates; D009841:Oligonucleotides; D013205:Staphylococcal Protein A
keywords: Affinity tags; Antibody; Antibody fragments; Antibody-binding protein; Click chemistry; Cycloaddition; Diagnostics; Immobilization; Metal binding protein; Nucleotide binding protein; Orientation; Physical adsorption; Protein A; Separation; Site-selective; Site-specific; Staudinger ligation; Therapeutics