title: Mucilaginibacterpsychrotolerans sp. nov., isolated from peatlands.
abstract: A Gram-stain-negative, rod-shaped, non-flagellated, pink, cold-tolerant bacterial strain, NH7-4T, was isolated from the Riganqiao peatlands on the Tibetan Plateau. The 16S rRNA gene sequence of the novel isolate shared a pairwise similarity ranging from 96.84 to 93.02 % with type strains of species of the genus Mucilaginibacter. Growth of strain NH7-4T occurred between 0 and 30 °C and at pH 5.0-9.0, with an optimum growth temperature at 20 °C and an optimum pH for growth of approximately 7.0. The major isoprenoid quinone was MK-7. The major cellular fatty acids were summed feature 3 (C16 : 1ω7c and/or iso-C15 : 0 2-OH), iso-C15 : 0, iso-C17 : 0 3-OH, C16 : 0, iso-C15 : 0 3-OH and C16 : 1ω5c. The major polar lipid of strain NH7-4T was phosphatidylethanolamine. Strain NH7-4T did not assimilate any substrates in API 20NE strips without low concentrations of yeast extract being present and had a lower optimal growth temperature, which distinguished it from other type strains of species of the genus Mucilaginibacter. The DNA G+C content of strain NH7-4T was 48.6 mol%. Based on phylogenetic, phenotypic and chemotaxonomic data, strain NH7-4T (=JCM 30607T=CGMCC1.14937T) represents a novel species of the genus Mucilaginibacter for which the name Mucilaginibacter psychrotolerans sp. nov. is proposed.
authors.affiliation: College of Geographic Sciences, Nanjing Normal University, Nanjing, PR China.
authors.forename: Yongcui
authors.initials: Y
authors.lastname: Deng
authors.affiliation: Key Laboratory of Tibetan Environment Changes and Land Surface Processes, Institute of Tibetan Plateau Research, Chinese Academy of Sciences, Beijing, PR China.|University of Chinese Academy of Sciences, Colledge of Resources and Environment, Beijing, PR China.|CAS Center for Excellence in Tibetan Plateau Earth Sciences, Beijing, PR China.
authors.forename: Liang
authors.initials: L
authors.lastname: Shen
authors.affiliation: Key Laboratory of Tibetan Environment Changes and Land Surface Processes, Institute of Tibetan Plateau Research, Chinese Academy of Sciences, Beijing, PR China.|CAS Center for Excellence in Tibetan Plateau Earth Sciences, Beijing, PR China.
authors.forename: Baiqin
authors.initials: B
authors.lastname: Xu
authors.affiliation: Key Laboratory of Tibetan Environment Changes and Land Surface Processes, Institute of Tibetan Plateau Research, Chinese Academy of Sciences, Beijing, PR China.|CAS Center for Excellence in Tibetan Plateau Earth Sciences, Beijing, PR China.|University of Chinese Academy of Sciences, Colledge of Resources and Environment, Beijing, PR China.
authors.forename: Yongqin
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Key Laboratory of Alpine Ecology and Biodiversity, Institute of Tibetan Plateau Research, Beijing, PR China.
authors.forename: Zhengquan
authors.initials: Z
authors.lastname: Gu
authors.affiliation: China General Microbiological Culture Collection Center, Institute of Microbiology Chinese Academy of Sciences, Beijing, PR China.
authors.forename: Hongcan
authors.initials: H
authors.lastname: Liu
authors.affiliation: China General Microbiological Culture Collection Center, Institute of Microbiology Chinese Academy of Sciences, Beijing, PR China.
authors.forename: Yuguang
authors.initials: Y
authors.lastname: Zhou
mesh_terms: D015373:Bacterial Typing Techniques; D041963:Bacteroidetes / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D010714:Phosphatidylethanolamines / Q000737:chemistry; D010802:Phylogeny; D010858:Pigmentation; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D018609:Tibet; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000737:chemistry; D053833:Wetlands
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010714:Phosphatidylethanolamines; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; C483858:phosphatidylethanolamine; C062629:menaquinone 7