title: Urinary excretion of uric acid, allantoin, and 8-OH-Deoxyguanosine in uricase-knockout mice.
abstract: Although uricase-knockout (Uox KO) mice are reported to develop uric acid (UA) nephropathy, those that mature without severe nephropathy could be useful for research into purine metabolism in humans. In this study, we measured the urinary excretion of creatinine, UA, allantoin, and 8-hydroxy-2'-deoxyguanosine (8-OHdG) collected from Uox KO mice housed in metabolic cages. UA and allantoin were determined using liquid chromatography-mass spectrometry and creatinine and 8-OHdG were measured with a commercial kit. Uox KO mice excreted significantly higher levels of UA than wild-type mice (C57BL/6), while the excretion of allantoin was significantly lower. Urinary allantoin was detected in Uox KO mice despite a lack of uricase, which is the same as in humans. In contrast to the elevated levels of UA, the daily excretion of 8-OHdG, an oxidative stress marker, was lower in Uox KO mice. UA is thought to act as an anti-oxidizing agent in humans; thus, these results show that Uox KO mice are potential animal models for research into human purine metabolism.
authors.affiliation: a Laboratory of Biomedical and Analytical Sciences, Faculty of Pharma-Science, Teikyo University , Tokyo , Japan.
authors.forename: K
authors.initials: K
authors.lastname: Inazawa
authors.affiliation: a Laboratory of Biomedical and Analytical Sciences, Faculty of Pharma-Science, Teikyo University , Tokyo , Japan.
authors.forename: S
authors.initials: S
authors.lastname: Yamaguchi
authors.affiliation: b Laboratory of Human Physiology & Pathology, Faculty of Pharma-Science, Teikyo University , Tokyo , Japan.
authors.forename: M
authors.initials: M
authors.lastname: Hosoyamada
authors.affiliation: a Laboratory of Biomedical and Analytical Sciences, Faculty of Pharma-Science, Teikyo University , Tokyo , Japan.
authors.forename: T
authors.initials: T
authors.lastname: Fukuuchi
authors.affiliation: b Laboratory of Human Physiology & Pathology, Faculty of Pharma-Science, Teikyo University , Tokyo , Japan.
authors.forename: N H
authors.initials: NH
authors.lastname: Tomioka
authors.affiliation: a Laboratory of Biomedical and Analytical Sciences, Faculty of Pharma-Science, Teikyo University , Tokyo , Japan.
authors.forename: N
authors.initials: N
authors.lastname: Yamaoka
authors.affiliation: a Laboratory of Biomedical and Analytical Sciences, Faculty of Pharma-Science, Teikyo University , Tokyo , Japan.
authors.forename: K
authors.initials: K
authors.lastname: Kaneko
mesh_terms: D000080242:8-Hydroxy-2'-Deoxyguanosine; D000481:Allantoin / Q000652:urine*; D000818:Animals; D003849:Deoxyguanosine / Q000031:analogs & derivatives* / Q000652:urine; D005260:Female; D008297:Male; D057507:Mice, 129 Strain; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D014503:Urate Oxidase / Q000235:genetics* / Q000378:metabolism; D014527:Uric Acid / Q000652:urine*
chemical_list: D014527:Uric Acid; D000481:Allantoin; D000080242:8-Hydroxy-2'-Deoxyguanosine; D014503:Urate Oxidase; D003849:Deoxyguanosine
keywords: 8-OH-deoxyguanosine; Uricase-KO mice; allantoin; uric acid; urinary excretion