title: Fructosylation induced structural changes in mammalian DNA examined by biophysical techniques.
abstract: Glycosylation of DNA, proteins, lipids, etc. by reducing sugars, can lead to the formation of advanced glycation end products (AGEs). These products may accumulate and involve in the pathogenesis of a number of diseases, contributing to tissue injury via several mechanisms. In this study, fructosylation of calf thymus dsDNA was carried out with varying concentrations of fructose. The neo-structure of fructosylated-DNA was studied by various biophysical techniques and morphological characterization. Fructosylated-DNA showed hyperchromicity, increase in fluorescence intensity and decrease in melting temperature. The CD signal of modified-DNA shifted in the direction of higher wavelength indicative of structural changes in DNA. FTIR results indicated shift in specific band positions in fructosylated-DNA. Morphological characterization of fructosylated-DNA exhibited strand breakage and aggregation. The results suggest that the structure and conformation of DNA may be altered under high concentrations of fructose.
authors.affiliation: Department of Biochemistry, Faculty of Medicine, Aligarh Muslim University, Aligarh 202002, India.
authors.forename: Asif
authors.initials: A
authors.lastname: Zaman
authors.affiliation: Department of Biochemistry, Faculty of Medicine, Aligarh Muslim University, Aligarh 202002, India.
authors.forename: Zarina
authors.initials: Z
authors.lastname: Arif
authors.affiliation: Department of Biochemistry, Faculty of Medicine, Aligarh Muslim University, Aligarh 202002, India. Electronic address: kalam786@rediffmail.com.
authors.forename: Khursheed
authors.initials: K
authors.lastname: Alam
mesh_terms: D000468:Alkalies / Q000737:chemistry; D000818:Animals; D001703:Biophysics / Q000379:methods*; D002417:Cattle; D002942:Circular Dichroism; D004247:DNA / Q000737:chemistry* / Q000378:metabolism* / Q000648:ultrastructure; D000067493:Dynamic Light Scattering; D005453:Fluorescence; D005632:Fructose / Q000378:metabolism*; D006031:Glycosylation; D057446:Hydrodynamics; D008322:Mammals / Q000378:metabolism*; D009691:Nucleic Acid Denaturation; D013056:Spectrophotometry, Ultraviolet; D017550:Spectroscopy, Fourier Transform Infrared; D013696:Temperature; D014732:Vibration
chemical_list: D000468:Alkalies; D005632:Fructose; D004247:DNA; C427203:calf thymus DNA
keywords: DNA; DNA-AGEs; Fructosylation