title: Effects of MeJA on Arabidopsis metabolome under endogenous JA deficiency.
abstract: Jasmonates (JAs) play important roles in plant growth, development and defense. Comprehensive metabolomics profiling of plants under JA treatment provides insights into the interaction and regulation network of plant hormones. Here we applied high resolution mass spectrometry based metabolomics approach on Arabidopsis wild type and JA synthesis deficiency mutant opr3. The effects of exogenous MeJA treatment on the metabolites of opr3 were investigated. More than 10000 ion signals were detected and more than 2000 signals showed significant variation in different genotypes and treatment groups. Multivariate statistic analyses (PCA and PLS-DA) were performed and a differential compound library containing 174 metabolites with high resolution precursor ion-product ions pairs was obtained. Classification and pathway analysis of 109 identified compounds in this library showed that glucosinolates and tryptophan metabolism, amino acids and small peptides metabolism, lipid metabolism, especially fatty acyls metabolism, were impacted by endogenous JA deficiency and exogenous MeJA treatment. These results were further verified by quantitative reverse transcription PCR (RT-qPCR) analysis of 21 related genes involved in the metabolism of glucosinolates, tryptophan and α-linolenic acid pathways. The results would greatly enhance our understanding of the biological functions of JA.
authors.affiliation: State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, China Agricultural University, Beijing, 100193, China.
authors.forename: Jingjing
authors.initials: J
authors.lastname: Cao
authors.affiliation: College of Resources and Environmental Sciences, China Agricultural University, Beijing, 100193, China.
authors.forename: Mengya
authors.initials: M
authors.lastname: Li
authors.affiliation: State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, China Agricultural University, Beijing, 100193, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Chen
authors.affiliation: College of Resources and Environmental Sciences, China Agricultural University, Beijing, 100193, China.
authors.forename: Pei
authors.initials: P
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, China Agricultural University, Beijing, 100193, China.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Li
mesh_terms: D000085:Acetates / Q000494:pharmacology*; D017360:Arabidopsis / Q000187:drug effects / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000378:metabolism; D016000:Cluster Analysis; D003517:Cyclopentanes / Q000494:pharmacology*; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D017343:Genes, Plant; D005961:Glucosinolates / Q000378:metabolism; D050356:Lipid Metabolism / Q000187:drug effects; D055442:Metabolome / Q000187:drug effects*; D010088:Oxidoreductases / Q000172:deficiency / Q000378:metabolism; D054883:Oxylipins / Q000494:pharmacology*; D010455:Peptides / Q000378:metabolism; D025341:Principal Component Analysis; D060888:Real-Time Polymerase Chain Reaction; D014364:Tryptophan / Q000378:metabolism; D017962:alpha-Linolenic Acid / Q000378:metabolism
chemical_list: D000085:Acetates; D029681:Arabidopsis Proteins; D003517:Cyclopentanes; D005961:Glucosinolates; D054883:Oxylipins; D010455:Peptides; D017962:alpha-Linolenic Acid; C011006:jasmonic acid; D014364:Tryptophan; C072239:methyl jasmonate; C416525:OPR3 protein, Arabidopsis; D010088:Oxidoreductases