title: Isolation and characterization of adenoviruses infecting endangered golden snub-nosed monkeys (Rhinopithecus roxellana).
abstract: Adenoviruses are important pathogens with the potential for interspecies transmission between humans and non-human primates. Although many adenoviruses have been identified in monkeys, the knowledge of these viruses from the Colobinae members is quite limited.
  
  
  
  We conducted a surveillance of viral infection in endangered golden snub-nosed monkeys (Rhinopithecus roxellana) in the subfamily Colobinae in China, and found that 5.1% of sampled individuals were positive for adenovirus. One of the adenoviruses (SAdV-WIV19) was successfully isolated and its full-length genome was sequenced. The full-length genome of WIV19 is 33,562 bp in size, has a G + C content of 56.2%, and encodes 35 putative genes. Sequence analysis revealed that this virus represents a novel species in the genus Mastadenovirus. Diverse cell lines, including those of human origin, were susceptible to WIV19.
  
  
  
  We report the first time the isolation and full-length genomic characterization of an adenovirus from the subfamily Colobinae.
authors.affiliation: Key Laboratory of Special Pathogens and Center for Emerging Infectious Diseases, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, China.|University of Chinese Academy of Sciences, Beijing, China.
authors.forename: Bing
authors.initials: B
authors.lastname: Tan
authors.affiliation: Key Laboratory of Special Pathogens and Center for Emerging Infectious Diseases, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, China.
authors.forename: Li-Jun
authors.initials: LJ
authors.lastname: Wu
authors.affiliation: Key Laboratory of Special Pathogens and Center for Emerging Infectious Diseases, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, China.
authors.forename: Xing-Lou
authors.initials: XL
authors.lastname: Yang
authors.affiliation: Key Laboratory of Special Pathogens and Center for Emerging Infectious Diseases, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, China.
authors.forename: Bei
authors.initials: B
authors.lastname: Li
authors.affiliation: Key Laboratory of Special Pathogens and Center for Emerging Infectious Diseases, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.affiliation: Monitoring Center of Wildlife Diseases and Resource of Hubei Province, Wuhan, China.
authors.forename: Yong-Song
authors.initials: YS
authors.lastname: Lei
authors.affiliation: Monitoring Center of Wildlife Diseases and Resource of Hubei Province, Wuhan, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Li
authors.affiliation: Monitoring Center of Wildlife Diseases and Resource of Hubei Province, Wuhan, China.
authors.forename: Guo-Xiang
authors.initials: GX
authors.lastname: Yang
authors.affiliation: Monitoring Center of Wildlife Diseases and Resource of Hubei Province, Wuhan, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Chen
authors.affiliation: Monitoring Center of Wildlife Diseases and Resource of Hubei Province, Wuhan, China.
authors.forename: Guang
authors.initials: G
authors.lastname: Chen
authors.affiliation: Key Laboratory of Special Pathogens and Center for Emerging Infectious Diseases, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, China.
authors.forename: Han-Zhong
authors.initials: HZ
authors.lastname: Wang
authors.affiliation: Key Laboratory of Special Pathogens and Center for Emerging Infectious Diseases, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, China. zlshi@wh.iov.cn.|University of Chinese Academy of Sciences, Beijing, China. zlshi@wh.iov.cn.|Present Address: Key Laboratory of Special Pathogens and Biosafety, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, 430071, China. zlshi@wh.iov.cn.
authors.forename: Zheng-Li
authors.identifier: 0000-0001-8089-163X
authors.initials: ZL
authors.lastname: Shi
mesh_terms: D000256:Adenoviridae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D000257:Adenoviridae Infections / Q000453:epidemiology / Q000662:veterinary* / Q000821:virology; D000818:Animals; D001482:Base Composition; D002681:China / Q000453:epidemiology; D016000:Cluster Analysis; D016673:Colobinae / Q000821:virology*; D023061:Gene Order; D005814:Genes, Viral; D016679:Genome, Viral; D010802:Phylogeny; D015995:Prevalence; D018419:Primate Diseases / Q000453:epidemiology* / Q000821:virology*; D017422:Sequence Analysis, DNA
keywords: Golden snub-nosed monkey; Mastadenovirus; Simian adenovirus