title: Immunohistochemical Staining of Histological Fragments Derived from Salivary Gland Tumour Fine-Needle Biopsy Aspirates.
abstract: OBJECTIVE
  The aim of this study was to describe a method for analysing histological fragments derived from fine- needle aspirate biopsy (FNAB) of salivary gland tumours (SGTs), and to evaluate the use of immunohistochemistry (IHC) on them.
  
  
  METHODS
  We reviewed all 509 FNAB pathology reports taken from SGTs at Helsinki University Hospital, Finland, between 1999 and 2009. In 51% of the cases (n = 209) "histo-fragments" had been obtained and 31 had been further analysed by IHC. Of these, 25 (81%) were available for review. We evaluated the benefit of IHC by relating its added value to the preoperative cytological diagnosis and its accuracy compared with the postoperative histological diagnosis.
  
  
  RESULTS
  Most of the samples analysed by IHC were assigned a malignant diagnosis, with 12 different types of malignancy represented. IHC was advantageous in 76% of the cases. In the 108 studies using IHC in this series, antibodies to 36 different antigens were used.
  
  
  CONCLUSIONS
  Analysis of histo-fragments in FNABs using IHC can be valuable in specific differential diagnostics and raises diagnostic accuracy in SGTs.
authors.affiliation: Department of Otorhinolaryngology - Head and Neck Surgery, Helsinki University Hospital, University of Helsinki, Helsinki, Finland.
authors.forename: Marie
authors.initials: M
authors.lastname: Lundberg
authors.forename: Berent
authors.initials: B
authors.lastname: Munsterhjelm
authors.forename: Antti
authors.initials: A
authors.lastname: Mäkitie
authors.forename: Ilmo
authors.initials: I
authors.lastname: Leivo
mesh_terms: D000235:Adenolymphoma / Q000175:diagnosis* / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D008949:Adenoma, Pleomorphic / Q000175:diagnosis* / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D014408:Biomarkers, Tumor / Q000235:genetics* / Q000378:metabolism; D044963:Biopsy, Fine-Needle; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D003937:Diagnosis, Differential; D015870:Gene Expression; D006801:Humans; D007150:Immunohistochemistry; D058965:MART-1 Antigen / Q000235:genetics / Q000378:metabolism; D009369:Neoplasms / Q000175:diagnosis* / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D012189:Retrospective Studies; D012468:Salivary Gland Neoplasms / Q000175:diagnosis* / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D012469:Salivary Glands / Q000378:metabolism / Q000473:pathology; D012680:Sensitivity and Specificity; D014157:Transcription Factors
chemical_list: D014408:Biomarkers, Tumor; D004268:DNA-Binding Proteins; D058965:MART-1 Antigen; C504003:TTF1 protein, human; D014157:Transcription Factors