title: Neutralizing human recombinant antibodies against herpes simplex virus type 1 glycoproteins B from a phage-displayed scFv antibody library.
abstract: The HSV-1 envelope glycoprotein B (gB) plays a critical role in virus entry into host cells. Neutralizing antibodies can therefore potentially prevent virus entry into target cells and cell-to-cell spread of infection. Our present study focused on the selection of neutralizing single-chain Fv (scFv) antibodies of a phage-displayed nonimmune human scFv antibody library against gB of HSV-1. To enrich specific scFvs, two phage antibodies were isolated against amino acid residues 31-43 derived from the N-terminal part of gB using panning technique. Two scFvs, scFv-gB1and scFv-gB2, with frequencies of 45% and 20% were obtained from scFv clones after performing PCR and MvaI fingerprinting. In phage ELISA analysis, both gB1and gB2scFvs demonstrated high reactivity with the gB peptide. In the neutralization assay, scFv-gB1and scFv-gB2represented neutralizing effects of 55% and 59%, respectively. Upon further enhancement of the neutralizing effects of these antibodies, they can be considered as new potential alternatives in the treatment and prophylaxis of HSV-1 infections.
authors.affiliation: Recombinant Antibody Laboratory, Department of Immunology, School of Advanced Medical Sciences and Technologies, Shiraz University of Medical Sciences, Shiraz, Iran; Student research Committee, Shiraz University of Medical Sciences, Shiraz, Iran.
authors.forename: Vahid
authors.initials: V
authors.lastname: Bagheri
authors.affiliation: Recombinant Antibody Laboratory, Department of Immunology, School of Advanced Medical Sciences and Technologies, Shiraz University of Medical Sciences, Shiraz, Iran; Shiraz HIV/AIDS Research Center, Shiraz University of Medical Sciences, Shiraz, Iran. Electronic address: nejatollf@sums.ac.ir.
authors.forename: Foroogh
authors.initials: F
authors.lastname: Nejatollahi
authors.affiliation: Student Research Committee, Immunology Research Center, Department of Immunology and Allergy, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
authors.forename: Seyed Alireza
authors.initials: SA
authors.lastname: Esmaeili
authors.affiliation: Student Research Committee, Nanotechnology Research Center, Department of Medical Biotechnology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
authors.forename: Amir Abbas
authors.initials: AA
authors.lastname: Momtazi
authors.affiliation: Department of Bacteriology and Virology, Shiraz University of Medical Sciences, Shiraz, Iran; Shiraz HIV/AIDS Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
authors.forename: Mohamad
authors.initials: M
authors.lastname: Motamedifar
authors.affiliation: Neurogenic Inflammation Research Center, Mashhad University of Medical Sciences, Mashhad, Iran; Biotechnology Research Center, Mashhad University of Medical Sciences, Mashhad, Iran.
authors.forename: Amirhossein
authors.initials: A
authors.lastname: Sahebkar
mesh_terms: D000818:Animals; D057134:Antibodies, Neutralizing / Q000276:immunology*; D002522:Chlorocebus aethiops; D006561:Herpes Simplex / Q000276:immunology / Q000628:therapy / Q000821:virology*; D018259:Herpesvirus 1, Human / Q000276:immunology*; D006801:Humans; D007167:Immunotherapy; D019151:Peptide Library; D057127:Single-Chain Antibodies / Q000276:immunology*; D014709:Vero Cells; D014759:Viral Envelope Proteins / Q000276:immunology*
chemical_list: D057134:Antibodies, Neutralizing; D019151:Peptide Library; D057127:Single-Chain Antibodies; D014759:Viral Envelope Proteins; C040495:glycoprotein B, Simplexvirus
keywords: Glycoprotein B; Herpes simplex virus type 1; Immunotherapy; Neonatal herpes; Neutralizing antibody; Single-chain antibody