title: Overexpression of microRNA-365 inhibits breast cancer cell growth and chemo-resistance through GALNT4.
abstract: A role of microRNA-365 (miR-365) in the carcinogenesis of breast cancer remains undetermined. In the current study, we addressed this question.
  
  
  
  We examined the levels of miR-365 in breast cancer tissue, compared to the paired adjacent non-tumor breast tissue from the patients. We also examined the effects of miR-365 modification on the breast cancer growth and sensitivity to Fluorouracil, as well as the underlying mechanisms.
  
  
  
  The miR-365 levels in breast cancer tissue were significantly lower than those in control normal breast tissues. Transfection with the miR-365 mimic decreased the breast cancer cell growth and increased their sensitivity to Fluorouracil, while transfection with the antisense of miR-365 (as-miR-365) increased breast cancer cell growth and decreased their sensitivity to Fluorouracil. Bioinformatics analyses showed that GALNT4 was a potential target gene of miR-365. The luciferase activities assay and Western blot verified that miR-365 targeted GALNT4 mRNA to modulate its protein levels.
  
  
  
  Our study suggests that downregulation of miR-365 may facilitate carcinogenesis of breast cancer cells via GALNT4, and thus miR-365 appears to be a promising target for breast cancer therapy.
authors.affiliation: Department of General Surgery, Shidong Hospital, Yangpu District, Shanghai, China. zyzj656@yeah.net.
authors.forename: J
authors.initials: J
authors.lastname: Zhang
authors.forename: Z
authors.initials: Z
authors.lastname: Zhang
authors.forename: Q
authors.initials: Q
authors.lastname: Wang
authors.forename: X-J
authors.initials: XJ
authors.lastname: Xing
authors.forename: Y
authors.initials: Y
authors.lastname: Zhao
mesh_terms: D001943:Breast Neoplasms / Q000235:genetics*; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D035683:MicroRNAs / Q000235:genetics*; D017350:N-Acetylgalactosaminyltransferases; D000097763:Polypeptide N-acetylgalactosaminyltransferase
chemical_list: D035683:MicroRNAs; D017350:N-Acetylgalactosaminyltransferases; D000097763:Polypeptide N-acetylgalactosaminyltransferase; C546819:MIRN365 microRNA, human