title: Isolation and Characterization of Exosomes Released by EBV-Immortalized Cells.
abstract: Epstein-Barr virus is an oncogenic herpesvirus associated with several human malignancies. Although the details of the molecular steps involved in EBV-mediated cell transformation and immune evasion are not fully known, a number of viral products, including EBV latent proteins and non-protein coding RNAs have been shown to be involved, directly or indirectly in these processes. In recent years, a growing body of data indicates that some viruses are able to transport selected products to neighboring cells and induce biological changes by exploiting the exosome secretory pathway. Exosomes are nanovesicles secreted by virtually all cell types and present in most body fluids. Here, we describe the protocols used in our laboratory to isolate and characterize exosomes from EBV-infected, noninfected, and transfected cell lines.
authors.affiliation: Department of Microbiology and Immunology, College of Medicine and Health Sciences, United Arab Emirates University, Tawam Hospital Campus, 17666, Al Ain, United Arab Emirates. g_khan@uaeu.ac.ae.
authors.forename: Gulfaraz
authors.initials: G
authors.lastname: Khan
authors.affiliation: Department of Microbiology and Immunology, College of Medicine and Health Sciences, United Arab Emirates University, Tawam Hospital Campus, 17666, Al Ain, United Arab Emirates.
authors.forename: Waqar
authors.initials: W
authors.lastname: Ahmed
mesh_terms: D001402:B-Lymphocytes / Q000378:metabolism / Q000473:pathology / Q000821:virology; D015415:Biomarkers; D015153:Blotting, Western; D002458:Cell Fractionation; D002461:Cell Line, Transformed; D002472:Cell Transformation, Viral; D002478:Cells, Cultured; D020031:Epstein-Barr Virus Infections / Q000150:complications / Q000378:metabolism* / Q000821:virology*; D055354:Exosomes / Q000378:metabolism* / Q000648:ultrastructure; D004854:Herpesvirus 4, Human / Q000502:physiology*; D006801:Humans; D008565:Membrane Proteins / Q000378:metabolism; D060149:Tetraspanin 30 / Q000378:metabolism
chemical_list: D015415:Biomarkers; D008565:Membrane Proteins; D060149:Tetraspanin 30; C106354:flotillins
keywords: CD63; Differential ultracentrifugation; EBV; Electron microscopy; Exosomes; Flotillin; RT-PCR; Western blotting