title: The function of activatable cell-penetrating peptides in human intrahepatic bile duct epithelial cells.
abstract: This study aimed to investigate the function of Activatable Cell-Penetrating Peptides (ACPP) in detecting the changes of human intrahepatic bile duct epithelial cell(hIBDEC). ACPP, which target matrix metalloproteinases, were constructed. All were labeled with FITC and Gd-DTPA at the N-terminal. Fluorescence microscopy was used to observe the fluorescence intensity inside hIBDEC after stimulating with different concentrations of LPS and incubating with different concentrations of ACPP to determine the optimal concentration range for LPS stimulation and the optimal concentration for FITC-ACPP effect. Flow cytometry and magnetic resonance imaging were used to detect fluorescence signal intensity and nuclear magnetic resonance signal intensity, respectively, after stimulating with different concentrations of LPS. LPS stimulation time and ACPP incubation time were also evaluated, and variance analysis was conducted to analyze intracellular signal change characteristics for every group. Activatable Cell-Penetrating Peptides (ACPP), which were marked with FITC and Gd-DTPA had target-penetrating activity. The intracellular signal intensity gradually increased with the increase in LPS stimulation time and ACPP incubation time within a certain range; however, it did not increase with the increase of LPS concentration. ACPP can be used for imaging hIBDEC with epithelial-mesenchymal transition.
authors.affiliation: Department of Hepatobiliary Surgery, Affiliated Hospital of Zunyi Medical College, Zunyi, 563000, China.
authors.forename: Kui
authors.initials: K
authors.lastname: Tu
authors.affiliation: Department of Hepatobiliary Surgery, Affiliated Hospital of Zunyi Medical College, Zunyi, 563000, China. 386421696@qq.com.
authors.forename: Lijin
authors.initials: L
authors.lastname: Zhao
authors.affiliation: Department of Hepatobiliary Surgery, Affiliated Hospital of Zunyi Medical College, Zunyi, 563000, China.
authors.forename: Jin
authors.initials: J
authors.lastname: Gu
authors.affiliation: Department of Hepatobiliary Surgery, Affiliated Hospital of Zunyi Medical College, Zunyi, 563000, China.
authors.forename: Pengzhan
authors.initials: P
authors.lastname: Yan
authors.affiliation: Department of Hepatobiliary Surgery, Affiliated Hospital of Zunyi Medical College, Zunyi, 563000, China.
authors.forename: Fang
authors.initials: F
authors.lastname: Wang
authors.affiliation: Department of Hepatobiliary Surgery, Affiliated Hospital of Zunyi Medical College, Zunyi, 563000, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Cao
mesh_terms: D001653:Bile Ducts, Intrahepatic / Q000166:cytology* / Q000000981:diagnostic imaging*; D057846:Cell-Penetrating Peptides / Q000032:analysis*; D004847:Epithelial Cells / Q000166:cytology*; D058750:Epithelial-Mesenchymal Transition; D005434:Flow Cytometry / Q000379:methods; D006801:Humans; D008070:Lipopolysaccharides / Q000494:pharmacology; D008279:Magnetic Resonance Imaging / Q000379:methods; D013194:Staining and Labeling
chemical_list: D057846:Cell-Penetrating Peptides; D008070:Lipopolysaccharides
keywords: Activable cell-penetrating peptide; Epithelial–mesenchymal transition; Human intrahepatic bile duct epithelial cell; Lipopolysaccharide; Molecular imaging