title: Integrin α5β1 Inhibition by CLT-28643 Reduces Postoperative Wound Healing in a Mouse Model of Glaucoma Filtration Surgery.
abstract: To evaluate the therapeutic potential of the small molecule integrin α5β1 inhibitor, CLT-28643, to improve the filtering surgery outcome in a mouse model. Different dose regimens and administration routes of the inhibitor were compared with mitomycin C (MMC), the gold standard in clin ical practice.
  
  
  
  The efficacy of CLT-28643 on surgical outcome was studied in a mouse model for filtering surgery (n = 40 eyes from 20 mice per group). Single and repeated subconjunctival (SCJ) injections (1 or 2 μg) and topical eye drops (10 μg) of the integrin inhibitor were compared with 2-minute administration of MMC 0.02%. Bleb size, survival, and signs of toxicity were examined until 28 days after surgery. Immunohistochemical analysis of angiogenesis, inflammation, collagen deposition, and integrin α5β1 expression were performed on postoperative days 3, 8, 14, and 28. A masked observer performed all the assessments.
  
  
  
  Immunostaining showed that integrin α5β1 was highly expressed in the bleb at early time-points after surgery and that CLT-28643 inhibited this upregulation. Efficacy was shown to be dose-dependent for the integrin inhibitor CLT-28643 for bleb area and survival, and the wound healing process. While 2-μg single injection of CLT-28643 improved bleb characteristics in a similar way as 10-μg administered by eye drops and MMC, repeated injections of 2 μg showed superior efficacy compared to MMC, with no corneal toxicity.
  
  
  
  Administration of the integrin α5β1 inhibitor CLT-28643 has therapeutic potential as an adjunct to glaucoma surgery, possibly with a superior efficacy and tolerability compared with MMC when used at the optimal dose.
authors.affiliation: Laboratory of Ophthalmology, KU Leuven, Leuven, Belgium.
authors.forename: Tine
authors.initials: T
authors.lastname: Van Bergen
authors.affiliation: Clanotech, Stockholm, Sweden.
authors.forename: Grit
authors.initials: G
authors.lastname: Zahn
authors.affiliation: Clanotech, Stockholm, Sweden.
authors.forename: Patrizia
authors.initials: P
authors.lastname: Caldirola
authors.affiliation: Clanotech, Stockholm, Sweden 3International Pharm-Med Ltd., Bramhall, United Kingdom.
authors.forename: Mario
authors.initials: M
authors.lastname: Fsadni
authors.affiliation: Clanotech, Stockholm, Sweden.
authors.forename: Ninus
authors.initials: N
authors.lastname: Caram-Lelham
authors.affiliation: Laboratory of Ophthalmology, KU Leuven, Leuven, Belgium 4Department of Ophthalmology, University Hospitals Leuven, Leuven, Belgium.
authors.forename: Evelien
authors.initials: E
authors.lastname: Vandewalle
authors.affiliation: Laboratory of Ophthalmology, KU Leuven, Leuven, Belgium 5Unit of Animal Physiology and Neurobiology, Biology Department, KU Leuven, Leuven, Belgium.
authors.forename: Lieve
authors.initials: L
authors.lastname: Moons
authors.affiliation: Laboratory of Ophthalmology, KU Leuven, Leuven, Belgium 4Department of Ophthalmology, University Hospitals Leuven, Leuven, Belgium.
authors.forename: Ingeborg
authors.initials: I
authors.lastname: Stalmans
mesh_terms: D000634:Aminoquinolines / Q000008:administration & dosage*; D000818:Animals; D003228:Conjunctiva / Q000378:metabolism*; D004195:Disease Models, Animal; D018463:Filtering Surgery; D005500:Follow-Up Studies; D005901:Glaucoma / Q000378:metabolism / Q000503:physiopathology / Q000601:surgery*; D007150:Immunohistochemistry; D007267:Injections; D039081:Integrin alpha5beta1 / Q000037:antagonists & inhibitors* / Q000096:biosynthesis; D051379:Mice; D008810:Mice, Inbred C57BL; D009883:Ophthalmic Solutions; D011184:Postoperative Period; D014945:Wound Healing / Q000187:drug effects*
chemical_list: D000634:Aminoquinolines; C000613971:CLT-28643; D039081:Integrin alpha5beta1; D009883:Ophthalmic Solutions