title: The Roles of Alpha-Momorcharin and Jasmonic Acid in Modulating the Response ofMomordica charantiatoCucumber Mosaic Virus.
abstract: Alpha-momorcharin (α-MMC) is a type-I ribosome inactivating protein with a molecular weight of 29 kDa that is found inMomordica charantia, and has been shown to be effective against a broad range of human viruses as well as having anti-tumor activities. However, the role of endogenous α-MMC under viral infection and the mechanism of the anti-viral activities of α-MMC in plants are still unknown. To study the effect of α-MMC on plant viral defense and how α-MMC increases plant resistance to virus, theM. charantia-cucumber mosaic virus(CMV) interaction system was investigated. The results showed that the α-MMC level was positively correlated with the resistance ofM. charantiato CMV. α-MMC treatment could alleviate photosystem damage and enhance the ratio of glutathione/glutathione disulfide inM. charantiaunder CMV infection. The relationship of α-MMC and defense related phytohormones, and their roles in plant defense were further investigated. α-MMC treatment led to a significant increase of jasmonic acid (JA) and vice versa, while there was no obvious relevance between salicylic acid and α-MMC. In addition, reactive oxygen species (ROS) were induced in α-MMC-pretreated plants, in a similar way to the ROS burst in JA-pretreated plants. The production of ROS in both ibuprofen (JA inhibitor) and (α-MMC+ibuprofen)-pretreated plants was reduced markedly, leading to a greater susceptibility ofM. charantiato CMV. Our results indicate that the anti-viral activities of α-MMC inM. charantiamay be accomplished through the JA related signaling pathway.
authors.affiliation: Ministry of Education Key Laboratory for Bio-Resource and Eco-Environment, College of Life Science, Sichuan University Chengdu, China.
authors.forename: Ting
authors.initials: T
authors.lastname: Yang
authors.affiliation: School of Medical Laboratory Science, Chengdu Medical College Chengdu, China.
authors.forename: Yao
authors.initials: Y
authors.lastname: Meng
authors.affiliation: Ministry of Education Key Laboratory for Bio-Resource and Eco-Environment, College of Life Science, Sichuan University Chengdu, China.
authors.forename: Li-Juan
authors.initials: LJ
authors.lastname: Chen
authors.affiliation: Ministry of Education Key Laboratory for Bio-Resource and Eco-Environment, College of Life Science, Sichuan University Chengdu, China.
authors.forename: Hong-Hui
authors.initials: HH
authors.lastname: Lin
authors.affiliation: Ministry of Education Key Laboratory for Bio-Resource and Eco-Environment, College of Life Science, Sichuan University Chengdu, China.
authors.forename: De-Hui
authors.initials: DH
authors.lastname: Xi
keywords: Cucumber mosaic virus; Momordica charantia; alpha-momorcharin; jasmonic acid; reactive oxygen species; salicylic acid