title: miR-15b Inhibits the Progression of Glioblastoma Cells Through Targeting Insulin-like Growth Factor Receptor 1.
abstract: The microRNAs (miRNAs) have been suggested as a tumor suppressor in recent years. miR-15b was reported to exert an anti-oncogenic role in the proliferation, migration, and invasion of diverse tumor cells. However, the mechanisms underlying miR-15b-mediated biology of glioblastoma are still unclear. In the present study, the expression of miR-15b was down-regulated in glioblastoma tumor tissues and U87 and U251 cells, but insulin-like growth factor receptor 1 (IGF1R) expression became up-regulated in these tumor tissues and cells (all p < 0.001). Furthermore, IGF1R expression was inversely associated with miR-15b expression. Notably, patients with lower miR-15b expression have a much shorter survival period compared with high expression (log-rank test p = 0.045). In vitro data demonstrated that miR-15b mimics inhibited the proliferation, cell cycle arrest, and invasion of U87 and U251 cells. Besides, we validated IGF1R as a direct target of miR-15b using dual luciferase assays, and IGF1R plasmids partially abrogated miR-15b mimics inhibited cell proliferation. In vivo, miR-15b mimics indeed repressed cell proliferation in mouse xenograft model. In conclusion, our study demonstrated that miR-15b inhibits the progression of glioblastoma cells through targeting IGF1R, and miR-15b can be recommended as a tumor suppressor in the progression of glioblastoma.
authors.affiliation: Department of Neurosurgery, Weifang Yidu Central Hospital, No. 4138, Linglongshan Road, Qingzhou, Shandong, 262500, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Wang
authors.affiliation: Department of Neurosurgery, Weifang Yidu Central Hospital, No. 4138, Linglongshan Road, Qingzhou, Shandong, 262500, China.
authors.forename: Huaqiang
authors.initials: H
authors.lastname: Liu
authors.affiliation: Department of Neurosurgery, Weifang Yidu Central Hospital, No. 4138, Linglongshan Road, Qingzhou, Shandong, 262500, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Tian
authors.affiliation: Department of Neurosurgery, Weifang Yidu Central Hospital, No. 4138, Linglongshan Road, Qingzhou, Shandong, 262500, China.
authors.forename: Fachen
authors.initials: F
authors.lastname: Wang
authors.affiliation: Department of Neurosurgery, Weifang Yidu Central Hospital, No. 4138, Linglongshan Road, Qingzhou, Shandong, 262500, China.
authors.forename: Liangbo
authors.initials: L
authors.lastname: Han
authors.affiliation: Department of Neurosurgery, Weifang Yidu Central Hospital, No. 4138, Linglongshan Road, Qingzhou, Shandong, 262500, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.affiliation: Department of Neurosurgery, Weifang Yidu Central Hospital, No. 4138, Linglongshan Road, Qingzhou, Shandong, 262500, China. baiyayidu@163.com.
authors.forename: Yun-An
authors.initials: YA
authors.lastname: Bai
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000235:genetics*; D015536:Down-Regulation; D015972:Gene Expression Regulation, Neoplastic; D016147:Genes, Tumor Suppressor; D005909:Glioblastoma / Q000235:genetics* / Q000378:metabolism* / Q000401:mortality; D006801:Humans; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D035683:MicroRNAs / Q000378:metabolism*; D017526:Receptor, IGF Type 1; D017451:Receptors, Somatomedin / Q000378:metabolism*; D015854:Up-Regulation; D023041:Xenograft Model Antitumor Assays
chemical_list: C000597236:IGF1R protein, human; C505361:MIRN15 microRNA, human; D035683:MicroRNAs; D017451:Receptors, Somatomedin; D017526:Receptor, IGF Type 1
keywords: Glioblastoma; IGF1R; miR-15b