title: Effect of common and experimental anti-tuberculosis treatments onMycobacterium tuberculosisgrowing as biofilms.
abstract: Much is known regarding the antibiotic susceptibility of planktonic cultures ofMycobacterium tuberculosis, the bacterium responsible for the lung disease tuberculosis (TB). As planktonically-grownM. tuberculosisare unlikely to be entirely representative of the bacterium during infection, we set out to determine how effective a range of anti-mycobacterial treatments were againstM. tuberculosisgrowing as a biofilm, a bacterial phenotype known to be more resistant to antibiotic treatment. Light levels from bioluminescently-labelledM. tuberculosisH37Rv (strain BSG001) were used as a surrogate for bacterial viability, and were monitored before and after one week of treatment. After treatment, biofilms were disrupted, washed and inoculated into fresh broth and plated onto solid media to rescue any surviving bacteria. We found that in this phenotypic stateM. tuberculosiswas resistant to the majority of the compounds tested. Minimum inhibitory concentrations (MICs) increased by 20-fold to greater than 1,000-fold, underlying the potential of this phenotype to cause significant problems during treatment.
authors.affiliation: Molecular Medicine and Pathology, University of Auckland, Auckland, New Zealand; Bioluminescent Superbugs Lab, University of Auckland, Auckland, New Zealand; Maurice Wilkins Centre for Molecular Biodiscovery, Auckland, New Zealand.
authors.forename: James P
authors.initials: JP
authors.lastname: Dalton
authors.affiliation: Molecular Medicine and Pathology, University of Auckland, Auckland, New Zealand; Bioluminescent Superbugs Lab, University of Auckland, Auckland, New Zealand.
authors.forename: Benedict
authors.initials: B
authors.lastname: Uy
authors.affiliation: School of Chemical Sciences, University of Auckland , Auckland , New Zealand.
authors.forename: Narisa
authors.initials: N
authors.lastname: Phummarin
authors.affiliation: Maurice Wilkins Centre for Molecular Biodiscovery, Auckland, New Zealand; School of Chemical Sciences, University of Auckland, Auckland, New Zealand.
authors.forename: Brent R
authors.initials: BR
authors.lastname: Copp
authors.affiliation: Maurice Wilkins Centre for Molecular Biodiscovery, Auckland, New Zealand; Auckland Cancer Society Research Centre, University of Auckland, Auckland, New Zealand.
authors.forename: William A
authors.initials: WA
authors.lastname: Denny
authors.affiliation: Molecular Medicine and Pathology, University of Auckland , Auckland , New Zealand.
authors.forename: Simon
authors.initials: S
authors.lastname: Swift
authors.affiliation: Molecular Medicine and Pathology, University of Auckland, Auckland, New Zealand; Bioluminescent Superbugs Lab, University of Auckland, Auckland, New Zealand; Maurice Wilkins Centre for Molecular Biodiscovery, Auckland, New Zealand.
authors.forename: Siouxsie
authors.initials: S
authors.lastname: Wiles
keywords: Ascorbic acid; Biofilm; Fluoroanthranilic acid; MTB; Mycobacterium tuberculosis; Nitroimidazole; Pellicle; TB; Tryptophan biosynthesis