title: Comparative transcriptome analysis between an evolved abscisic acid-overproducing mutant Botrytis cinerea TBC-A and its ancestral strain Botrytis cinerea TBC-6.
abstract: Abscisic acid (ABA) is a classical phytohormone which plays an important role in plant stress resistance. Moreover, ABA is also found to regulate the activation of innate immune cells and glucose homeostasis in mammals. Therefore, this 'stress hormone' is of great importance to theoretical research and agricultural and medical applications. Botrytis cinerea is a well-known phytopathogenic ascomycete that synthesizes ABA via a pathway substantially different from higher plants. Identification of the functional genes involved in ABA biosynthesis in B. cinerea would be of special interest. We developed an ABA-overproducing mutant strain, B. cinerea TBC-A, previously and obtained a 41.5-Mb genome sequence of B. cinerea TBC-A. In this study, the transcriptomes of B. cinerea TBC-A and its ancestral strain TBC-6 were sequenced under identical fermentation conditions. A stringent comparative transcriptome analysis was performed to identify differentially expressed genes participating in the metabolic pathways related to ABA biosynthesis in B. cinerea. This study provides the first global view of the transcriptional changes underlying the very different ABA productivity of the B. cinerea strains and will expand our knowledge of the molecular basis for ABA biosynthesis in B. cinerea.
authors.affiliation: Key Laboratory of Environmental and Applied Microbiology, Chengdu Institute of Biology, the Chinese Academy of Sciences, Chengdu, 610041, P.R. China.|University of the Chinese Academy of Sciences, Beijing, 100049, P.R. China.
authors.forename: Zhongtao
authors.initials: Z
authors.lastname: Ding
authors.affiliation: Key Laboratory of Environmental and Applied Microbiology, Chengdu Institute of Biology, the Chinese Academy of Sciences, Chengdu, 610041, P.R. China.|University of the Chinese Academy of Sciences, Beijing, 100049, P.R. China.
authors.forename: Zhi
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Environmental and Applied Microbiology, Chengdu Institute of Biology, the Chinese Academy of Sciences, Chengdu, 610041, P.R. China.
authors.forename: Juan
authors.initials: J
authors.lastname: Zhong
authors.affiliation: Key Laboratory of Environmental and Applied Microbiology, Chengdu Institute of Biology, the Chinese Academy of Sciences, Chengdu, 610041, P.R. China.
authors.forename: Di
authors.initials: D
authors.lastname: Luo
authors.affiliation: Key Laboratory of Environmental and Applied Microbiology, Chengdu Institute of Biology, the Chinese Academy of Sciences, Chengdu, 610041, P.R. China.
authors.forename: Jinyan
authors.initials: J
authors.lastname: Zhou
authors.affiliation: Key Laboratory of Environmental and Applied Microbiology, Chengdu Institute of Biology, the Chinese Academy of Sciences, Chengdu, 610041, P.R. China.
authors.forename: Jie
authors.initials: J
authors.lastname: Yang
authors.affiliation: Key Laboratory of Environmental and Applied Microbiology, Chengdu Institute of Biology, the Chinese Academy of Sciences, Chengdu, 610041, P.R. China.
authors.forename: Liang
authors.initials: L
authors.lastname: Xiao
authors.affiliation: Key Laboratory of Environmental and Applied Microbiology, Chengdu Institute of Biology, the Chinese Academy of Sciences, Chengdu, 610041, P.R. China.
authors.forename: Dan
authors.initials: D
authors.lastname: Shu
authors.affiliation: Key Laboratory of Environmental and Applied Microbiology, Chengdu Institute of Biology, the Chinese Academy of Sciences, Chengdu, 610041, P.R. China.
authors.forename: Hong
authors.initials: H
authors.lastname: Tan
mesh_terms: D000040:Abscisic Acid / Q000096:biosynthesis* / Q000235:genetics; D020171:Botrytis / Q000235:genetics* / Q000378:metabolism; D019143:Evolution, Molecular; D020869:Gene Expression Profiling; D015966:Gene Expression Regulation, Fungal; D016681:Genome, Fungal; D053858:Metabolic Networks and Pathways / Q000235:genetics; D010935:Plant Diseases / Q000382:microbiology; D010937:Plant Growth Regulators / Q000096:biosynthesis* / Q000235:genetics; D059467:Transcriptome
chemical_list: D010937:Plant Growth Regulators; D000040:Abscisic Acid