title: MicroRNA-206 acts as a tumor suppressor in bladder cancer via targeting YRDC.
abstract: Accumulating evidence suggested that microRNA (miRNA) plays important regulatory roles in the initiation and development of various cancers. Previous study showed that microRNA-206 (miR-206) is dysregulated in human bladder cancer tissues, however, the biological function and underlying mechanisms of miR-206 in human bladder cancer remain unknown. In the present study, we aimed to investigate the clinical significance of miR-206 and its target gene YRDC in human bladder cancer, and to determine its effects on oncogenic phenotypes of this disease. Our results showed that miR-206 expression was downregulated significantly in bladder cancer tissues and cell lines compared with adjacent normal bladder tissues and human bladder epithelial immortalized SV-HUC-1 cell line, respectively. Overexpression of miR-206 reduced the expression of YRDC and inhibited bladder cancer cell proliferation, colony formation, migration, invasion and induced cell cycle arrest at G0/G1 phase. In addition, knockdown of YRDC exhibited similar effects with miR-206 overexpression in bladder cancer cells and restoration of YRDC partially reversed the effects of miR-206 in bladder cancer cells. These findings indicated that mir-206 might be a novel target for bladder cancer therapy by targeting YRDC.
authors.affiliation: Department of Urology, Shanghai Tenth People's Hospital, Tongji University 301 Yanchang Road, Shanghai 200072, China.
authors.forename: Bisheng
authors.initials: B
authors.lastname: Huang
authors.affiliation: Department of Urology, Shanghai Tenth People's Hospital, Tongji University 301 Yanchang Road, Shanghai 200072, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhai
authors.affiliation: Department of Urology, Shanghai Tenth People's Hospital, Tongji University 301 Yanchang Road, Shanghai 200072, China.
authors.forename: Guanghui
authors.initials: G
authors.lastname: Hu
authors.affiliation: Department of Urology, Shanghai Tenth People's Hospital, Tongji University301 Yanchang Road, Shanghai 200072, China; Department of First Clinical Medical College, Nanjing Medical UniversityNanjing, Jiangsu, China.
authors.forename: Chi
authors.initials: C
authors.lastname: Huang
authors.affiliation: Department of Urology, Shanghai Tenth People's Hospital, Tongji University 301 Yanchang Road, Shanghai 200072, China.
authors.forename: Tiancheng
authors.initials: T
authors.lastname: Xie
authors.affiliation: Department of Urology, Shanghai Tenth People's Hospital, Tongji University 301 Yanchang Road, Shanghai 200072, China.
authors.forename: Jingwei
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Department of Urology, Shanghai Tenth People's Hospital, Tongji University 301 Yanchang Road, Shanghai 200072, China.
authors.forename: Yunfei
authors.initials: Y
authors.lastname: Xu
keywords: YRDC; bladder cancer; miR-206; tumor suppressor