title: In vivo transmission of a plasmid containing the KPC-2 gene in a single patient.
abstract: Here we describe a case of in vivo horizontal interspecies transmission of a KPC-2-producing plasmid from a Klebsiella pneumoniae to an Enterobacter aerogenes strain in the same patient. The patient's gut flora initially contained a carbapenem-susceptible E. aerogenes strain and 10 days after admission a KPC-2-positive K. pneumoniae. Three months after admission, a KPC-2-positive E. aerogenes was identified in fecal surveillance cultures. This isolate was isogenic with the initial E. aerogenes and contained a KPC-2-coding plasmid identical to that of the K. pneumoniae. The patient developed bacteraemia by the KPC-2-positive K. pneumoniae 17 days after her first colonization. In vivo horizontal transmission of blaKPC-carrying plasmids between bacterial species underscores the importance of antibiotic stewardship along with implementation of infection control measures for the containment of KPC-producers.
authors.affiliation: 4th Department of Internal Medicine, Athens University School of Medicine, University General Hospital 'Attikon', 1 Rimini Street, 124 62 Chaidari, Athens, Greece.
authors.forename: Irene
authors.initials: I
authors.lastname: Galani
authors.affiliation: 4th Department of Internal Medicine, Athens University School of Medicine, University General Hospital 'Attikon', 1 Rimini Street, 124 62 Chaidari, Athens, Greece.
authors.forename: Theofano
authors.initials: T
authors.lastname: Panagea
authors.affiliation: 4th Department of Internal Medicine, Athens University School of Medicine, University General Hospital 'Attikon', 1 Rimini Street, 124 62 Chaidari, Athens, Greece.
authors.forename: Zoi
authors.initials: Z
authors.lastname: Chryssouli
authors.affiliation: 4th Department of Internal Medicine, Athens University School of Medicine, University General Hospital 'Attikon', 1 Rimini Street, 124 62 Chaidari, Athens, Greece. Electronic address: e.giamarellou@hygeia.gr.
authors.forename: Helen
authors.initials: H
authors.lastname: Giamarellou
authors.affiliation: 4th Department of Internal Medicine, Athens University School of Medicine, University General Hospital 'Attikon', 1 Rimini Street, 124 62 Chaidari, Athens, Greece.
authors.forename: Maria
authors.initials: M
authors.lastname: Souli
keywords: Carbapenemases; Conjugation; Enterobacter aerogenes; KPC; Plasmid; Transferable