title: Methods to Study Campylobacter jejuni Adherence to and Invasion of Host Epithelial Cells.
abstract: Measuring bacterial adherence and invasion of cells in vitro has enabled researchers to dissect the interactions of Campylobacter jejuni with eukaryotic cells. Numerous C. jejuni virulence determinants and host cell factors that contribute to the process of adherence, invasion, and immune modulation have been identified utilizing in vitro adherence and invasion assays. In this chapter, we describe the evaluation of C. jejuni adherence to and invasion of HeLa cells using the gentamicin-protection assay.
authors.affiliation: School of Molecular Biosciences, College of Veterinary Medicine, Washington State University, Pullman, WA, 99164-7520, USA.
authors.forename: Nicholas M
authors.initials: NM
authors.lastname: Negretti
authors.affiliation: School of Molecular Biosciences, College of Veterinary Medicine, Washington State University, Life Sciences Bldg. Room 302c, Pullman, WA, 99164-7520, USA. konkel@vetmed.wsu.edu.
authors.forename: Michael E
authors.initials: ME
authors.lastname: Konkel
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001422:Bacterial Adhesion / Q000187:drug effects; D001681:Biological Assay; D016123:Campylobacter jejuni / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism* / Q000472:pathogenicity; D002701:Chloramphenicol / Q000494:pharmacology; D003470:Culture Media / Q000737:chemistry; D005455:Fluorescent Antibody Technique; D005456:Fluorescent Dyes / Q000737:chemistry; D005839:Gentamicins / Q000494:pharmacology; D006367:HeLa Cells; D006801:Humans; D018613:Microscopy, Confocal; D013194:Staining and Labeling / Q000379:methods*
chemical_list: D000900:Anti-Bacterial Agents; D003470:Culture Media; D005456:Fluorescent Dyes; D005839:Gentamicins; D002701:Chloramphenicol
keywords: Adherence; Attachment; Bacteria-host cell interactions; Binding; Invasion