title: Proteomic profile of the Bradysia odoriphaga in response to the microbial secondary metabolite benzothiazole.
abstract: Benzothiazole, a microbial secondary metabolite, has been demonstrated to possess fumigant activity against Sclerotinia sclerotiorum, Ditylenchus destructor and Bradysia odoriphaga. However, to facilitate the development of novel microbial pesticides, the mode of action of benzothiazole needs to be elucidated. Here, we employed iTRAQ-based quantitative proteomics analysis to investigate the effects of benzothiazole on the proteomic expression of B. odoriphaga. In response to benzothiazole, 92 of 863 identified proteins in B. odoriphaga exhibited altered levels of expression, among which 14 proteins were related to the action mechanism of benzothiazole, 11 proteins were involved in stress responses, and 67 proteins were associated with the adaptation of B. odoriphaga to benzothiazole. Further bioinformatics analysis indicated that the reduction in energy metabolism, inhibition of the detoxification process and interference with DNA and RNA synthesis were potentially associated with the mode of action of benzothiazole. The myosin heavy chain, succinyl-CoA synthetase and Ca+-transporting ATPase proteins may be related to the stress response. Increased expression of proteins involved in carbohydrate metabolism, energy production and conversion pathways was responsible for the adaptive response of B. odoriphaga. The results of this study provide novel insight into the molecular mechanisms of benzothiazole at a large-scale translation level and will facilitate the elucidation of the mechanism of action of benzothiazole.
authors.affiliation: College of Plant Protection, Shandong Provincial Key Laboratory for Biology of Vegetable Diseases and Insect Pests, Shandong Agricultural University, Tai'an, Shandong 271018, P.R. China.
authors.forename: Yunhe
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: College of Plant Protection, Shandong Provincial Key Laboratory for Biology of Vegetable Diseases and Insect Pests, Shandong Agricultural University, Tai'an, Shandong 271018, P.R. China.
authors.forename: Kaidi
authors.initials: K
authors.lastname: Cui
authors.affiliation: College of Plant Protection, Shandong Provincial Key Laboratory for Biology of Vegetable Diseases and Insect Pests, Shandong Agricultural University, Tai'an, Shandong 271018, P.R. China.
authors.forename: Chunmei
authors.initials: C
authors.lastname: Xu
authors.affiliation: College of Plant Protection, Shandong Provincial Key Laboratory for Biology of Vegetable Diseases and Insect Pests, Shandong Agricultural University, Tai'an, Shandong 271018, P.R. China.
authors.forename: Qiuhong
authors.initials: Q
authors.lastname: Wang
authors.affiliation: College of Plant Protection, Shandong Provincial Key Laboratory for Biology of Vegetable Diseases and Insect Pests, Shandong Agricultural University, Tai'an, Shandong 271018, P.R. China.
authors.forename: Yao
authors.initials: Y
authors.lastname: Wang
authors.affiliation: College of Horticultural Science and Engineering, Shandong Agricultural University, Tai'an, Shandong 271018, PR China.
authors.forename: Zhengqun
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: College of Plant Protection, Shandong Provincial Key Laboratory for Biology of Vegetable Diseases and Insect Pests, Shandong Agricultural University, Tai'an, Shandong 271018, P.R. China.
authors.forename: Feng
authors.initials: F
authors.lastname: Liu
authors.affiliation: College of Plant Protection, Shandong Provincial Key Laboratory for Biology of Vegetable Diseases and Insect Pests, Shandong Agricultural University, Tai'an, Shandong 271018, P.R. China.
authors.forename: Wei
authors.initials: W
authors.lastname: Mu
mesh_terms: D000214:Acyl Coenzyme A / Q000378:metabolism; D000251:Adenosine Triphosphatases / Q000378:metabolism; D000818:Animals; D052160:Benzothiazoles / Q000494:pharmacology*; D002241:Carbohydrates / Q000494:pharmacology; D004175:Diptera / Q000187:drug effects* / Q000378:metabolism*; D007306:Insecticides / Q000494:pharmacology; D007814:Larva / Q000187:drug effects / Q000378:metabolism; D018995:Myosin Heavy Chains / Q000378:metabolism; D020543:Proteome / Q000378:metabolism*; D040901:Proteomics / Q000379:methods
chemical_list: D000214:Acyl Coenzyme A; D052160:Benzothiazoles; D002241:Carbohydrates; D007306:Insecticides; D020543:Proteome; C012046:succinyl-coenzyme A; D000251:Adenosine Triphosphatases; D018995:Myosin Heavy Chains; C005465:benzothiazole