title: Resveratrol attenuates MPP+-induced mitochondrial dysfunction and cell apoptosis via AKT/GSK-3β pathway in SN4741 cells.
abstract: Oxidative stress and mitochondrial dysfunction play crucial role in the dopaminergic neurons death, which is the pathological feature of Parkinson's disease (PD). Resveratrol (Res), a polyphenol derived from grapes and blueberries, has been reported to reduce oxidative stress injury and to restore mitochondrial function. In this study, we aimed to explore the underlying molecular mechanism of the beneficial effects of Res against MPP+- induced mitochondrial dysfunction and cell apoptosis in SN4741 cells. The data showed that Res significantly alleviated MPP+- induce cytotoxicity and restored MPP+- induced mitochondrial dysfunction in SN4741 cells. Moreover, Res rescued MPP+- induced a decline on the level of p-AKT, p-GSK-3βand the ratio of Bcl-2/Bax, and an elevation on the expression of Bax and caspase-3, 9. However, inhibition GSK-3β activity clearly abolished the protective effects of Res. Taken together, these results suggest that Res attenuates MPP+- induced mitochondrial dysfunction and cell apoptosis, and these protections may be achieved through AKT/GSK-3β pathway. These also indicate that Res could be a promising therapeutic agent for PD.
authors.affiliation: Department of Neurosurgery, Tangdu Hospital, The Fourth Military Medical University, 569 Xinsi Road, Xi'an, Shaanxi 710038, China.
authors.forename: Weijun
authors.initials: W
authors.lastname: Zeng
authors.affiliation: Department of Encephalopathy, Xi'an Hospital of Traditional Chinese Medicine, Xi'an, Shaanxi 710021, China; Research Center of Traditional Chinese Medicine, Xijing Hospital, Fourth Military Medical University, Xi'an, Shaanxi 710032, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.affiliation: Department of Neurosurgery, Tangdu Hospital, The Fourth Military Medical University, 569 Xinsi Road, Xi'an, Shaanxi 710038, China.
authors.forename: Fangfang
authors.initials: F
authors.lastname: Lu
authors.affiliation: Department of Neurosurgery, Tangdu Hospital, The Fourth Military Medical University, 569 Xinsi Road, Xi'an, Shaanxi 710038, China.
authors.forename: Li
authors.initials: L
authors.lastname: Gao
authors.affiliation: Department of Neurosurgery, Tangdu Hospital, The Fourth Military Medical University, 569 Xinsi Road, Xi'an, Shaanxi 710038, China. Electronic address: gaogd1954@163.com.
authors.forename: Guodong
authors.initials: G
authors.lastname: Gao
mesh_terms: D017209:Apoptosis / Q000187:drug effects* / Q000502:physiology; D053148:Caspase 3 / Q000378:metabolism; D002460:Cell Line; D059290:Dopaminergic Neurons / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D000071679:Glycogen Synthase Kinase 3 beta / Q000378:metabolism; D006801:Humans; D008928:Mitochondria / Q000187:drug effects* / Q000378:metabolism; D018696:Neuroprotective Agents / Q000494:pharmacology*; D051058:Oncogene Protein v-akt / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects; D010880:Piperidines / Q000494:pharmacology*; D011720:Pyrazoles / Q000494:pharmacology*; D000077185:Resveratrol; D015398:Signal Transduction / Q000187:drug effects*; D013267:Stilbenes / Q000494:pharmacology*
chemical_list: C524478:1,3-bis(4-hydroxyphenyl)-4-methyl-5-(4-(2-piperidinylethoxy)phenol)-1H-pyrazole; D018696:Neuroprotective Agents; D010880:Piperidines; D011720:Pyrazoles; D013267:Stilbenes; D000071679:Glycogen Synthase Kinase 3 beta; D051058:Oncogene Protein v-akt; C505407:CASP3 protein, human; D053148:Caspase 3; D000077185:Resveratrol
keywords: GSK-3β; Mitochondria; Neuronal apoptosis; Parkinson's disease; Resveratrol