title: Nanobioengineering and Characterization of a Novel Estrogen Receptor Biosensor.
abstract: We constructed an original supramolecular assembly on a surface of sensor composed of an innovative combination of an engineered cytochrome b5 and a modified nucleic acid bound to a synthetic lipid hemimembrane. The protein/DNA block, called (PDNA) 2, was synthesized and purified before its immobilization onto a hybrid bilayer reconstituted on a gold surface. Surface plasmon resonance (SPR) and atomic force microscopy (AFM) were engaged in parallel on the same substrates in order to better understand dynamic events that occur at the surface of the biosensor. Good correlations were obtained in terms of specificity and reversibility. These findings allow us to present a first application of such biosensor in the study of the interaction processes between nuclear receptor and DNA.
authors.affiliation: Institut FEMTO-ST, Université de Franche Comté, Clinical-Innovation Proteomic Platform, CNRS - 25044 Besançon cedex, France. alexandre.berthier@clipproteomic.fr.|Estrogènes, Expression Génique et Pathologies du Système Nerveux Central, EA3922, IFR 133, Université de Franche-Comté, UFR Sciences et Techniques, 16 route de Gray, 25030 Besançon cedex, France. alexandre.berthier@clipproteomic.fr.
authors.forename: Alexandre
authors.initials: A
authors.lastname: Berthier
authors.affiliation: Institut FEMTO-ST, Université de Franche Comté, Clinical-Innovation Proteomic Platform, CNRS - 25044 Besançon cedex, France. celine.caille@clipproteomic.fr.
authors.forename: Céline
authors.initials: C
authors.lastname: Elie-Caille
authors.affiliation: Institut Carnot Bourgogne UMR CNRS 5209, Nanosciences Department, University of Bourgogne, B.P. 47870, 21078 Dijon Cedex, France. eric.lesniewska@clipproteomic.fr.
authors.forename: Eric
authors.initials: E
authors.lastname: Lesniewska
authors.affiliation: Estrogènes, Expression Génique et Pathologies du Système Nerveux Central, EA3922, IFR 133, Université de Franche-Comté, UFR Sciences et Techniques, 16 route de Gray, 25030 Besançon cedex, France. regis.delagemourroux@clipproteomic.fr.
authors.forename: Régis
authors.initials: R
authors.lastname: Delage-Mourroux
authors.affiliation: Institut FEMTO-ST, Université de Franche Comté, Clinical-Innovation Proteomic Platform, CNRS - 25044 Besançon cedex, France. wilfrid.boireau@clipproteomic.fr.
authors.forename: Wilfrid
authors.initials: W
authors.lastname: Boireau
keywords: AFM; Nano-objects; Protein/DNA interaction.; SPR; molecular lego