title: Development of a competitive double antibody lateral flow assay for the detection of antibodies specific to glycoprotein B of Aujeszky's disease virus in swine sera.
abstract: Three lateral flow assays (LFAs) for the detection of antibodies against glycoprotein B (gB) of Aujeszky's disease virus (ADV) in swine sera: a competitive double antibody sandwich LFA without a preincubation step (CDAS-gB-LFA), a CDAS-gB-LFA with a preincubation step (pCDAS-gB-LFA), and a competitive direct gB-LFA have been developed and were compared with each other and with a gB-ELISA. The assays are based on monoclonal antibodies to immunodominant epitopes of ADV gB. The pCDAS-gB-LFA proved to be the most specific and sensitive assay to detect antibodies directed to ADV gB. The specificity and sensitivity of the pCDAS-gB-LFA with the use of an LFA reader for test line intensity measurements were 97.6 and 94.9%, respectively. The lower diagnostic sensitivity of the pCDAS-gB-LFA compared to a gB-ELISA reflects its reduced analytical sensitivity, which was shown in titration experiments with positive sera. The pCDAS-gB-LFA, using the reader-based and visual detection modes, showed good agreement in respect to specificity; however, the LFA reader detection provided a higher diagnostic and analytical sensitivity compared to visual detection. The developed pCDAS-gB-LFA is a rapid, sensitive, and specific method for the detection of antibodies to ADV gB and can be used for screening ADV-infected swine in unvaccinated herds.
authors.affiliation: Institute of Cell Biophysics, Russian Academy of Sciences, Pushchino, Moscow Region, 142290 Russia.
authors.forename: V V
authors.initials: VV
authors.lastname: Vrublevskaya
authors.affiliation: Institute of Cell Biophysics, Russian Academy of Sciences, Pushchino, Moscow Region, 142290 Russia.
authors.forename: V N
authors.initials: VN
authors.lastname: Afanasyev
authors.affiliation: Institute of Cell Biophysics, Russian Academy of Sciences, Pushchino, Moscow Region, 142290 Russia.
authors.forename: A A
authors.initials: AA
authors.lastname: Grinevich
authors.affiliation: Institute of Cell Biophysics, Russian Academy of Sciences, Pushchino, Moscow Region, 142290 Russia.
authors.forename: Yu Y
authors.initials: YY
authors.lastname: Skarga
authors.affiliation: State University "Dubna", Dubna, Moscow Region, 141982 Russia.
authors.forename: P P
authors.initials: PP
authors.lastname: Gladyshev
authors.affiliation: State University "Dubna", Dubna, Moscow Region, 141982 Russia.
authors.forename: S A
authors.initials: SA
authors.lastname: Ibragimova
authors.affiliation: Research Institute of Applied Acoustics, Dubna, Moscow Region, 141980 Russia.
authors.forename: D V
authors.initials: DV
authors.lastname: Krylsky
authors.affiliation: Institute of Cell Biophysics, Russian Academy of Sciences, Pushchino, Moscow Region, 142290 Russia. Electronic address: morenkov_o@mail.ru.
authors.forename: O S
authors.initials: OS
authors.lastname: Morenkov
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000914:Antibodies, Viral / Q000097:blood*; D000956:Antigens, Viral / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D006023:Glycoproteins / Q000276:immunology*; D011558:Herpesvirus 1, Suid / Q000276:immunology* / Q000302:isolation & purification; D009500:Neutralization Tests; D011557:Pseudorabies / Q000175:diagnosis* / Q000276:immunology; D012680:Sensitivity and Specificity; D013552:Swine; D013553:Swine Diseases / Q000175:diagnosis* / Q000276:immunology; D014759:Viral Envelope Proteins / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D000956:Antigens, Viral; D006023:Glycoproteins; D014759:Viral Envelope Proteins
keywords: Antibody detection; Aujeszky's disease virus; Competitive lateral flow assay; Glycoprotein B