title: Self-assembly of hexahistidine-tagged tobacco etch virus capsid protein into microfilaments that induce IgG2-specific response against a soluble porcine reproductive and respiratory syndrome virus chimeric protein.
abstract: Assembly of recombinant capsid proteins into virus-like particles (VLPs) still represents an interesting challenge in virus-based nanotechnologies. The structure of VLPs has gained importance for the development and design of new adjuvants and antigen carriers. The potential of Tobacco etch virus capsid protein (TEV CP) as adjuvant has not been evaluated to date.
  
  
  
  Two constructs for TEV CP expression in Escherichia coli were generated: a wild-type version (TEV-CP) and a C-terminal hexahistidine (His)-tagged version (His-TEV-CP). Although both versions were expressed in the soluble fraction of E. coli lysates, only His-TEV-CP self-assembled into micrometric flexuous filamentous VLPs. In addition, the His-tag enabled high yields and facilitated purification of TEV VLPs. These TEV VLPs elicited broader IgG2-specific antibody response against a novel porcine reproductive and respiratory syndrome virus (PRRSV) protein when compared to the potent IgG1 response induced by the protein alone.
  
  
  
  His-TEV CP was purified by immobilized metal affinity chromatography and assembled into VLPs, some of them reaching 2-μm length. TEV VLPs administered along with PRRSV chimeric protein changed the IgG2/IgG1 ratio against the chimeric protein, suggesting that TEV CP can modulate the immune response against a soluble antigen.
authors.affiliation: Unidad de Biotecnología Médica y Farmacéutica, Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco A.C., Normalistas 800, Colinas de la Normal, 44270, Guadalajara, Jalisco, Mexico.
authors.forename: Carlos Alberto
authors.initials: CA
authors.lastname: Manuel-Cabrera
authors.affiliation: Unidad de Biotecnología Médica y Farmacéutica, Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco A.C., Normalistas 800, Colinas de la Normal, 44270, Guadalajara, Jalisco, Mexico.
authors.forename: Alba Adriana
authors.initials: AA
authors.lastname: Vallejo-Cardona
authors.affiliation: Unidad de Biotecnología Médica y Farmacéutica, Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco A.C., Normalistas 800, Colinas de la Normal, 44270, Guadalajara, Jalisco, Mexico.
authors.forename: Eduardo
authors.initials: E
authors.lastname: Padilla-Camberos
authors.affiliation: Unidad de Biotecnología Médica y Farmacéutica, Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco A.C., Normalistas 800, Colinas de la Normal, 44270, Guadalajara, Jalisco, Mexico.
authors.forename: Rodolfo
authors.initials: R
authors.lastname: Hernández-Gutiérrez
authors.affiliation: Unidad de Biotecnología Médica y Farmacéutica, Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco A.C., Normalistas 800, Colinas de la Normal, 44270, Guadalajara, Jalisco, Mexico.
authors.forename: Sara Elisa
authors.initials: SE
authors.lastname: Herrera-Rodríguez
authors.affiliation: Unidad de Biotecnología Médica y Farmacéutica, Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco A.C., Normalistas 800, Colinas de la Normal, 44270, Guadalajara, Jalisco, Mexico. aortega@ciatej.mx.
authors.forename: Abel
authors.identifier: 0000-0002-5772-3950
authors.initials: A
authors.lastname: Gutiérrez-Ortega
mesh_terms: D008841:Actin Cytoskeleton / Q000378:metabolism; D000276:Adjuvants, Immunologic / Q000008:administration & dosage* / Q000378:metabolism; D000914:Antibodies, Viral / Q000097:blood*; D036022:Capsid Proteins / Q000008:administration & dosage* / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D007074:Immunoglobulin G / Q000097:blood; D019316:Porcine respiratory and reproductive syndrome virus / Q000276:immunology*; D017800:Potyvirus / Q000276:immunology*; D055503:Protein Multimerization; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D014765:Viral Vaccines / Q000276:immunology*; D022701:Virosomes / Q000008:administration & dosage* / Q000378:metabolism
chemical_list: D000276:Adjuvants, Immunologic; D000914:Antibodies, Viral; D036022:Capsid Proteins; D007074:Immunoglobulin G; D011994:Recombinant Proteins; D014765:Viral Vaccines; D022701:Virosomes
keywords: Adjuvant; Capsid protein; Chimeric protein; Hexahistidine tag; Tobacco etch virus; Virus-like particles