title: Deep sequencing of transcriptome profiling of GSTM2 knock-down in swine testis cells.
abstract: Glutathione-S-transferases mu 2 (GSTM2), a kind of important Phase II antioxidant enzyme of eukaryotes, is degraded by nonsense mediated mRNA decay due to a C27T substitution in the fifth exon of pigs. As a reproductive performance-related gene, GSTM2 is involved in embryo implantation, whereas, functional deficiency of GSTM2 induces pre- or post-natal death in piglets potentially. To have some insight into the role of GSTM2 in embryo development, high throughput RNA sequencing is performed using the swine testis cells (ST) with the deletion of GSTM2. Some embryo development-related genes are observed from a total of 242 differentially expressed genes, including STAT1, SRC, IL-8, DUSP family, CCL family and integrin family. GSTM2 affects expression of SRC, OPN, and SLCs. GSTM2 suppresses phosphorylation of STAT1 by binding to STAT1. In addition, as an important transcription factor, STAT1 regulates expression of uterus receptive-related genes including CCLs, IRF9, IFITs, MXs, and OAS. The present study provides evidence to molecular mechanism of GSTM2 modulating embryo development.
authors.affiliation: Key Laboratory of Swine Genetics and Breeding of Ministry of Agriculture &Key Laboratory of Agriculture Animal Genetics, Breeding and Reproduction of Ministry of Education, College of Animal Science, Huazhong Agricultural University, Wuhan, 430070 Hubei, P.R. China.
authors.forename: Yuqi
authors.initials: Y
authors.lastname: Lv
authors.affiliation: Key Laboratory of Swine Genetics and Breeding of Ministry of Agriculture &Key Laboratory of Agriculture Animal Genetics, Breeding and Reproduction of Ministry of Education, College of Animal Science, Huazhong Agricultural University, Wuhan, 430070 Hubei, P.R. China.|The Cooperative Innovation Center for Sustainable Pig Production, Huazhong Agricultural University, Wuhan, 430070 Hubei, P.R. China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Jin
authors.affiliation: Key Laboratory of Swine Genetics and Breeding of Ministry of Agriculture &Key Laboratory of Agriculture Animal Genetics, Breeding and Reproduction of Ministry of Education, College of Animal Science, Huazhong Agricultural University, Wuhan, 430070 Hubei, P.R. China.
authors.forename: Yongqiang
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: Key Laboratory of Swine Genetics and Breeding of Ministry of Agriculture &Key Laboratory of Agriculture Animal Genetics, Breeding and Reproduction of Ministry of Education, College of Animal Science, Huazhong Agricultural University, Wuhan, 430070 Hubei, P.R. China.
authors.forename: Jianjun
authors.initials: J
authors.lastname: Jin
authors.affiliation: Key Laboratory of Swine Genetics and Breeding of Ministry of Agriculture &Key Laboratory of Agriculture Animal Genetics, Breeding and Reproduction of Ministry of Education, College of Animal Science, Huazhong Agricultural University, Wuhan, 430070 Hubei, P.R. China.
authors.forename: Zhenfa
authors.initials: Z
authors.lastname: Ma
authors.affiliation: Key Laboratory of Swine Genetics and Breeding of Ministry of Agriculture &Key Laboratory of Agriculture Animal Genetics, Breeding and Reproduction of Ministry of Education, College of Animal Science, Huazhong Agricultural University, Wuhan, 430070 Hubei, P.R. China.|The Cooperative Innovation Center for Sustainable Pig Production, Huazhong Agricultural University, Wuhan, 430070 Hubei, P.R. China.
authors.forename: Zhuqing
authors.initials: Z
authors.lastname: Ren
mesh_terms: D000818:Animals; D004622:Embryo, Mammalian / Q000201:enzymology*; D047108:Embryonic Development; D020869:Gene Expression Profiling; D055785:Gene Knockdown Techniques; D005982:Glutathione Transferase / Q000172:deficiency* / Q000378:metabolism; D059014:High-Throughput Nucleotide Sequencing; D008297:Male; D013552:Swine; D013737:Testis / Q000196:embryology / Q000201:enzymology*; D059467:Transcriptome
chemical_list: D005982:Glutathione Transferase; C412602:glutathione S-transferase Mu 2