title: Hyperbaric oxygen protects mandibular condylar chondrocytes from interleukin-1β-induced apoptosis via the PI3K/AKT signaling pathway.
abstract: Objectives:Mandibular condylar chondrocyte apoptosis is mainly responsible for the development and progression of temporomandibular joint osteoarthritis (TMJ-OA). Interleukin-1β (IL-1β) generally serves an agent that induces chondrocyte apoptosis. Hyperbaric oxygen (HBO) treatment increases proteoglycan synthesisin vivo. We explore the protective effect of HBO on IL-1β-induced mandibular condylar chondrocyte apoptosis in rats and the potential molecular mechanisms.Methods:Chondrocytes were isolated from the TMJ of 3-4-week old Sprague-Dawley rats. The Cell Counting Kit-8 (CCK-8) assay was used to determine cell viability. The phosphorylated phosphoinositide-3 kinase (p-PI3K), phosphorylated AKT (p-Akt), type II collagen (COL2), and aggrecan (AGG) content was detected by immunofluorescence, immunocytochemistry and western blotting. The expression ofPi3k, Akt, Col2andAggmRNA was measured using real-time quantitative polymerase chain reaction (RT-qPCR).Results:HBO inhibited the cytotoxicity and apoptosis induced by IL-1β (10 ng/mL) in the mandibular condylar chondrocytes. HBO also decreased the IL-1β activity that decreased p-PI3K and p-AKT levels, and increased COL2 and AGG expression, with the net effect of suppressing extracellular matrix degradation.Conclusions:These data suggest that HBO may protect mandibular condylar chondrocytes against IL-1β-induced apoptosis via the PI3K/AKT signaling pathway, and that it may promote the expression of mandibular condylar chondrocyte extracellular matrix through the PI3K/AKT signaling pathway.
authors.affiliation: College of Stomatology, Shandong UniversityJinan City, Shandong, China; Shandong Provincial Key Laboratory of Oral Tissue RegenerationShandong, China.
authors.forename: Hang
authors.initials: H
authors.lastname: Chen
authors.affiliation: Department of Stomatology, Jinan Military General Hospital Shandong, China.
authors.forename: Gaoyi
authors.initials: G
authors.lastname: Wu
authors.affiliation: College of Stomatology, Shandong UniversityJinan City, Shandong, China; Shandong Provincial Key Laboratory of Oral Tissue RegenerationShandong, China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Sun
authors.affiliation: College of Stomatology, Shandong UniversityJinan City, Shandong, China; Shandong Provincial Key Laboratory of Oral Tissue RegenerationShandong, China.
authors.forename: Yabing
authors.initials: Y
authors.lastname: Dong
authors.affiliation: College of Stomatology, Shandong UniversityJinan City, Shandong, China; Shandong Provincial Key Laboratory of Oral Tissue RegenerationShandong, China.
authors.forename: Huaqiang
authors.initials: H
authors.lastname: Zhao
keywords: Hyperbaric oxygen; IL-1β; PI3K/AKT signaling; extracellular matrix