title: OXA-23 and ISAba1-OXA-66 class D β-lactamases in Acinetobacter baumannii isolates from companion animals.
abstract: Acinetobacter baumannii is recognised as a major pathogen of nosocomial infections that frequently show resistance to last-resort antimicrobials. To investigate whether A. baumannii from companion animals harbour carbapenem resistance mechanisms, 223 clinical isolates obtained from veterinary clinics between 2000 and 2013 in Germany were screened for carbapenem-non-susceptibility employing meropenem-containing Mueller-Hinton agar plates. Minimum inhibitory concentration (MIC) data were obtained using the VITEK®2 system. Assignment to international clones (ICs) was done by multiplex PCR or repetitive sequence-based PCR employing the DiversiLab system. Clonality was studied using pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST). Genes encoding carbapenemases and aminoglycoside-modifying enzymes were detected by PCR. In three samples from dogs, carbapenem-resistant A. baumannii carrying the blaOXA-23gene on plasmids and located on transposon Tn2008 were identified. The isolates belonged to sequence type ST1P(clonal complex CC1/IC1/pulsotype II) and ST10P(CC10/IC8/pulsotype IV) according to the Pasteur MLST scheme, and to ST231Ox(CC109) and ST585Ox(CC447) following the Oxford scheme. Insertion sequence ISAba1 was identified upstream of blaOXA-66in 58 A. baumannii isolates. MLST referred them to ST2P(CC2/IC2/pulsotypes I and III), ST208Ox, ST350Oxand ST556Ox(all CC118), respectively. PFGE suggested nosocomial spread of these highly related strains, which frequently demonstrated a multidrug-resistant phenotype, in one veterinary clinic. These data show that A. baumannii from companion animals reveal resistance determinants and clonal lineages of strains globally emerging in humans. This suggests an interspecies transmission and warrants molecular surveillance of A. baumannii in veterinary clinics to mitigate its further spread.
authors.affiliation: Institute of Hygiene and Infectious Diseases of Animals, Justus Liebig University Giessen, Frankfurter Str. 85-89, Giessen 35392, Germany. Electronic address: christa.ewers@vetmed.uni-giessen.de.
authors.forename: Christa
authors.initials: C
authors.lastname: Ewers
authors.affiliation: Institute of Hygiene and Infectious Diseases of Animals, Justus Liebig University Giessen, Frankfurter Str. 85-89, Giessen 35392, Germany.
authors.forename: Peter
authors.initials: P
authors.lastname: Klotz
authors.affiliation: Institute of Hygiene and Infectious Diseases of Animals, Justus Liebig University Giessen, Frankfurter Str. 85-89, Giessen 35392, Germany.
authors.forename: Ursula
authors.initials: U
authors.lastname: Leidner
authors.affiliation: Division of IDEXX Laboratories, Vet Med Labor GmbH, Mörikestr. 28/3, Ludwigsburg 71636, Germany.
authors.forename: Ivonne
authors.initials: I
authors.lastname: Stamm
authors.affiliation: Institute of Hygiene and Infectious Diseases of Animals, Justus Liebig University Giessen, Frankfurter Str. 85-89, Giessen 35392, Germany.
authors.forename: Ellen
authors.initials: E
authors.lastname: Prenger-Berninghoff
authors.affiliation: Institute for Medical Microbiology and Infection Control, Hospital of Johann Wolfgang Goethe University, Paul-Ehrlich-Str. 40, Frankfurt/Main 60596, Germany.
authors.forename: Stephan
authors.initials: S
authors.lastname: Göttig
authors.affiliation: Robert Koch Institute, Nordufer 20, Berlin 13353, Germany.
authors.forename: Torsten
authors.initials: T
authors.lastname: Semmler
authors.affiliation: Institute of Hygiene and Infectious Diseases of Animals, Justus Liebig University Giessen, Frankfurter Str. 85-89, Giessen 35392, Germany.
authors.forename: Sandra
authors.initials: S
authors.lastname: Scheufen
mesh_terms: D000151:Acinetobacter Infections / Q000453:epidemiology / Q000382:microbiology / Q000662:veterinary*; D040981:Acinetobacter baumannii / Q000145:classification / Q000201:enzymology* / Q000235:genetics / Q000302:isolation & purification*; D000818:Animals; D004251:DNA Transposable Elements; D016521:Electrophoresis, Gel, Pulsed-Field; D005798:Genes, Bacterial; D014644:Genetic Variation; D005858:Germany; D008826:Microbial Sensitivity Tests; D017720:Molecular Epidemiology; D058885:Multilocus Sequence Typing; D057805:Pets / Q000382:microbiology*; D010957:Plasmids; D016133:Polymerase Chain Reaction; D001618:beta-Lactamases / Q000032:analysis* / Q000235:genetics*
chemical_list: D004251:DNA Transposable Elements; D001618:beta-Lactamases
keywords: Acinetobacter; Animal; Carbapenems; ISAba1; OXA; Tn2008