title: Postprandial sleep mechanics inDrosophila.
abstract: Food consumption is thought to induce sleepiness. However, little is known about how postprandial sleep is regulated. Here, we simultaneously measured sleep and food intake of individual flies and found a transient rise in sleep following meals. Depending on the amount consumed, the effect ranged from slightly arousing to strongly sleep inducing. Postprandial sleep was positively correlated with ingested volume, protein, and salt-but not sucrose-revealing meal property-specific regulation. Silencing of leucokinin receptor (Lkr) neurons specifically reduced sleep induced by protein consumption. Thermogenetic stimulation of leucokinin (Lk) neurons decreased whereas Lk downregulation by RNAi increased postprandial sleep, suggestive of an inhibitory connection in the Lk-Lkr circuit. We further identified a subset of non-leucokininergic cells proximal to Lkr neurons that rhythmically increased postprandial sleep when silenced, suggesting that these cells are cyclically gated inhibitory inputs to Lkr neurons. Together, these findings reveal the dynamic nature of postprandial sleep.
authors.affiliation: Department of Metabolism and Aging, The Scripps Research Institute, Jupiter, United States.|Program in Integrative Biology and Neuroscience, Florida Atlantic University, Jupiter, United States.|Department of Neuroscience, The Scripps Research Institute, Jupiter, United States.
authors.forename: Keith R
authors.initials: KR
authors.lastname: Murphy
authors.affiliation: Department of Metabolism and Aging, The Scripps Research Institute, Jupiter, United States.
authors.forename: Sonali A
authors.initials: SA
authors.lastname: Deshpande
authors.affiliation: Program in Integrative Biology and Neuroscience, Florida Atlantic University, Jupiter, United States.
authors.forename: Maria E
authors.initials: ME
authors.lastname: Yurgel
authors.affiliation: Department of Metabolism and Aging, The Scripps Research Institute, Jupiter, United States.
authors.forename: James P
authors.initials: JP
authors.lastname: Quinn
authors.affiliation: Department of Metabolism and Aging, The Scripps Research Institute, Jupiter, United States.
authors.forename: Jennifer L
authors.initials: JL
authors.lastname: Weissbach
authors.affiliation: Program in Integrative Biology and Neuroscience, Florida Atlantic University, Jupiter, United States.
authors.forename: Alex C
authors.initials: AC
authors.lastname: Keene
authors.affiliation: Program in Integrative Biology and Neuroscience, Florida Atlantic University, Jupiter, United States.
authors.forename: Ken
authors.initials: K
authors.lastname: Dawson-Scully
authors.affiliation: Radcliffe Institute for Advanced Study, Harvard University, Cambridge, United States.|JP Scott Center for Neuroscience, Mind and Behavior, Bowling Green State University, Bowling Green, United States.
authors.forename: Robert
authors.initials: R
authors.lastname: Huber
authors.affiliation: Department of Neuroscience, The Scripps Research Institute, Jupiter, United States.
authors.forename: Seth M
authors.initials: SM
authors.lastname: Tomchik
authors.affiliation: Department of Metabolism and Aging, The Scripps Research Institute, Jupiter, United States.|Department of Neuroscience, The Scripps Research Institute, Jupiter, United States.
authors.forename: William W
authors.identifier: 0000-0002-4003-7356
authors.initials: WW
authors.lastname: Ja
mesh_terms: D000818:Animals; D004330:Drosophila / Q000502:physiology*; D004435:Eating; D009474:Neurons / Q000502:physiology; D019518:Postprandial Period; D012890:Sleep
keywords: D. melanogaster; behavior; feeding; neurogenetics; neuroscience; nutrition; sleep