title: First report of a clinical isolate of Leclercia adecarboxylata harbouring multiple resistance genes in Uruguay and review of the literature.
abstract: Here we report the detection of a Leclercia adecarboxylata strain, isolated from a case of osteomyelitis, harbouring multiple antibiotic resistance genes encoded on a 450-kb IncHI1/HI2 conjugative plasmid (pLa12). The plasmid carried a complex class 1 integron with the genetic array intI1-aac(6')-Ib-cr-blaOXA-1-catB3-arr3-qacEΔ1-sul1-ISCR1; in addition, a blaDHA-1-like allele linked to ampR-qacEΔ1-sul1 as well as blaSHV-12, blaTEM-1and qnrB4-like genes were found. To the best of our knowledge, this is the first report of L. adecarboxylata harbouring transferable resistance genes to quinolones, chloramphenicol and rifampicin as well as a plasmidic class C β-lactamase.
authors.affiliation: Departamento de Bacteriología y Virología, Instituto de Higiene, Facultad de Medicina, Universidad de la República, Montevideo, Uruguay.
authors.forename: Virginia
authors.initials: V
authors.lastname: García-Fulgueiras
authors.affiliation: Sección Bacteriología, Laboratorio Central, Hospital Pasteur, Administración de los Servicios de Salud del Estado, Larravide S/N, Montevideo, Uruguay.
authors.forename: Verónica
authors.initials: V
authors.lastname: Seija
authors.affiliation: Departamento de Bacteriología y Virología, Instituto de Higiene, Facultad de Medicina, Universidad de la República, Montevideo, Uruguay.
authors.forename: Paula
authors.initials: P
authors.lastname: Aguerrebere
authors.affiliation: Departamento de Bacteriología y Virología, Instituto de Higiene, Facultad de Medicina, Universidad de la República, Montevideo, Uruguay.
authors.forename: Nicolás F
authors.initials: NF
authors.lastname: Cordeiro
authors.affiliation: Departamento de Bacteriología y Virología, Instituto de Higiene, Facultad de Medicina, Universidad de la República, Montevideo, Uruguay. Electronic address: rvignoli@higiene.edu.uy.
authors.forename: Rafael
authors.initials: R
authors.lastname: Vignoli
keywords: ESBL; Extended-spectrum β-lactamase; Integron; PMQR; Plasmid-mediated quinolone resistance