title: TMED3 promotes hepatocellular carcinoma progression via IL-11/STAT3 signaling.
abstract: Transmembrane p24 trafficking protein 3(TMED3) is a metastatic suppressor in colon cancer, but its function in the progression of hepatocellular carcinoma (HCC) is unknown. Here, we report that TMED3 was up-regulated in HCC and portal vein tumor thrombus. TMED3 up-regulation in HCC was significantly correlated with aggressive characteristics and predicted poor prognosis in HCC patients. TMED3 overexpression in HCC cell lines promoted cell migration and invasion. In contrast, TMED3 knockdown suppressed HCC metastasis both in vitro and in vivo. Gene microarray analysis revealed decreased IL-11 expression in TMED3-knockdown cells. We propose that TMED3 promotes HCC metastasis through IL-11/STAT3 signaling. Taken together, these findings demonstrate that TMED3 promotes HCC metastasis and is a potential prognostic biomarker in HCC.
authors.affiliation: The Third Department of Hepatic Surgery, Eastern Hepatobiliary Surgery Hospital, Second Military Medical University, 225 Changhai Road, Shanghai 200438, China.
authors.forename: Hao
authors.initials: H
authors.lastname: Zheng
authors.affiliation: The Third Department of Hepatic Surgery, Eastern Hepatobiliary Surgery Hospital, Second Military Medical University, 225 Changhai Road, Shanghai 200438, China.|Department of Health Statistics, Second Military Medical University, 800 Xiangyin Road, Shanghai 200433, China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Yang
authors.affiliation: The Third Department of Hepatic Surgery, Eastern Hepatobiliary Surgery Hospital, Second Military Medical University, 225 Changhai Road, Shanghai 200438, China.|Department of Microbiology, Shanghai Key Laboratory of Medical Biodefense, Second Military Medical University, 800 Xiangyin Road, Shanghai 200433, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Han
authors.affiliation: Department of Oncology, Shanghai Tongren Hospital, Shanghai Jiaotong University, 1111 Xianxia Road, Shanghai 200336, China.
authors.forename: Wei-Hua
authors.initials: WH
authors.lastname: Jiang
authors.affiliation: Department of Medical Oncology, Jinling Hospital, 305 Zhongshan East Road, Nanjing, Jiangsu 210000, China.
authors.forename: Cheng
authors.initials: C
authors.lastname: Chen
authors.affiliation: The Third Department of Hepatic Surgery, Eastern Hepatobiliary Surgery Hospital, Second Military Medical University, 225 Changhai Road, Shanghai 200438, China.
authors.forename: Meng-Chao
authors.initials: MC
authors.lastname: Wang
authors.affiliation: Department of Microbiology, Shanghai Key Laboratory of Medical Biodefense, Second Military Medical University, 800 Xiangyin Road, Shanghai 200433, China.
authors.forename: Rong
authors.initials: R
authors.lastname: Gao
authors.affiliation: Department of Computer Science, Rensselaer Polytechnic Institute, 110 8th Street, Troy, NY, 12180, United States.
authors.forename: Shuai
authors.initials: S
authors.lastname: Li
authors.affiliation: The Third Department of Hepatic Surgery, Eastern Hepatobiliary Surgery Hospital, Second Military Medical University, 225 Changhai Road, Shanghai 200438, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Tian
authors.affiliation: The Third Department of Hepatic Surgery, Eastern Hepatobiliary Surgery Hospital, Second Military Medical University, 225 Changhai Road, Shanghai 200438, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Wang
authors.affiliation: Department of Oncology, Shanghai Tongren Hospital, Shanghai Jiaotong University, 1111 Xianxia Road, Shanghai 200336, China.
authors.forename: Li-Jun
authors.initials: LJ
authors.lastname: Ma
authors.affiliation: Department of Microbiology, Shanghai Key Laboratory of Medical Biodefense, Second Military Medical University, 800 Xiangyin Road, Shanghai 200433, China.
authors.forename: Hao
authors.initials: H
authors.lastname: Ren
authors.affiliation: The Third Department of Hepatic Surgery, Eastern Hepatobiliary Surgery Hospital, Second Military Medical University, 225 Changhai Road, Shanghai 200438, China.
authors.forename: Wei-Ping
authors.initials: WP
authors.lastname: Zhou
mesh_terms: D000818:Animals; D006528:Carcinoma, Hepatocellular / Q000175:diagnosis / Q000378:metabolism* / Q000556:secondary; D002465:Cell Movement; D018450:Disease Progression; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D055785:Gene Knockdown Techniques; D006801:Humans; D017370:Interleukin-11 / Q000378:metabolism*; D008113:Liver Neoplasms / Q000175:diagnosis / Q000378:metabolism* / Q000556:secondary; D008297:Male; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D008875:Middle Aged; D009362:Neoplasm Metastasis / Q000503:physiopathology; D050796:STAT3 Transcription Factor / Q000378:metabolism*; D015854:Up-Regulation; D033921:Vesicular Transport Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: C508611:IL11 protein, human; D017370:Interleukin-11; D050796:STAT3 Transcription Factor; C494086:STAT3 protein, human; C577103:TMED3 protein, human; D033921:Vesicular Transport Proteins