title: In situ forming pluronic® F127/chitosan hydrogel limits metronidazole transmucosal absorption.
abstract: The objective of this work is to design topically-applied thermosensitive and mucoadhesive hydrogel containing metronidazole (MTZ) for the treatment of Trichomonas vaginalis infections. Hydrogel composed of pluronic® F127 (20wt%), chitosan (1wt%) and metronidazole MTZ (0.7wt%) mixture showed its ability to decrease by a factor 4 MTZ flux and apparent permeability absorption through vaginal mucosa. The impact of hydrogel on transmucosal penetration of MTZ was evaluated ex vivo on excised porcine vaginal mucosa mounted on Franz diffusion cell. The anti-T. vaginalis activity of MTZ formulated into F127/chitosan hydrogel was preserved since the viability curve evaluated in vitro was similar to MTZ solution.
authors.affiliation: Institut Galien Paris Sud, UMR CNRS 8612, Univ. Paris-Sud, Université Paris-Saclay, Faculté de Pharmacie, 5, rue J-B. Clément, 92296 Châtenay-Malabry, France; BioCIS « Biomolécules: Conception, Isolement, Synthèse » - « Chimiothérapie Antiparasitaire », UMR CNRS 8076, Univ. Paris-Sud, Université Paris-Saclay, Faculté de Pharmacie, 5, rue J.B. Clément, 92296 Châtenay-Malabry cedex, France.
authors.forename: Sophia
authors.initials: S
authors.lastname: Malli
authors.affiliation: BioCIS « Biomolécules: Conception, Isolement, Synthèse » - « Chimiothérapie Antiparasitaire », UMR CNRS 8076, Univ. Paris-Sud, Université Paris-Saclay, Faculté de Pharmacie, 5, rue J.B. Clément, 92296 Châtenay-Malabry cedex, France.
authors.forename: Christian
authors.initials: C
authors.lastname: Bories
authors.affiliation: BioCIS « Biomolécules: Conception, Isolement, Synthèse » - « Chimiothérapie Antiparasitaire », UMR CNRS 8076, Univ. Paris-Sud, Université Paris-Saclay, Faculté de Pharmacie, 5, rue J.B. Clément, 92296 Châtenay-Malabry cedex, France.
authors.forename: Bénédicte
authors.initials: B
authors.lastname: Pradines
authors.affiliation: BioCIS « Biomolécules: Conception, Isolement, Synthèse » - « Chimiothérapie Antiparasitaire », UMR CNRS 8076, Univ. Paris-Sud, Université Paris-Saclay, Faculté de Pharmacie, 5, rue J.B. Clément, 92296 Châtenay-Malabry cedex, France.
authors.forename: Philippe M
authors.initials: PM
authors.lastname: Loiseau
authors.affiliation: Institut Galien Paris Sud, UMR CNRS 8612, Univ. Paris-Sud, Université Paris-Saclay, Faculté de Pharmacie, 5, rue J-B. Clément, 92296 Châtenay-Malabry, France.
authors.forename: Gilles
authors.initials: G
authors.lastname: Ponchel
authors.affiliation: Institut Galien Paris Sud, Junior Member of the Institut Universitaire de France, UMR CNRS 8612, Univ. Paris-Sud, Université Paris-Saclay, Faculté de Pharmacie, 5, rue J-B. Clément, 92296 Châtenay-Malabry, France. Electronic address: kawthar.bouchemal@u-psud.fr.
authors.forename: Kawthar
authors.initials: K
authors.lastname: Bouchemal
mesh_terms: D000818:Animals; D000981:Antiprotozoal Agents / Q000493:pharmacokinetics*; D048271:Chitosan / Q000737:chemistry*; D005260:Female; D020100:Hydrogels / Q000737:chemistry*; D008795:Metronidazole / Q000493:pharmacokinetics*; D009092:Mucous Membrane / Q000378:metabolism*; D010539:Permeability; D020442:Poloxamer / Q000737:chemistry*; D013552:Swine; D014621:Vagina
chemical_list: D000981:Antiprotozoal Agents; D020100:Hydrogels; D020442:Poloxamer; D008795:Metronidazole; D048271:Chitosan
keywords: Chitosan; Hydrogel; Mucosal passage; Pluronic® F127; Thermosensitive; Trichomonas vaginalis