title: Stimuli-Responsive Cucurbit[7]uril-Mediated BSA Nanoassembly for Uptake and Release of Doxorubicin.
abstract: We report the construction of a non-toxic nanoassembly of bovine serum albumin (BSA) protein and the cucurbit[7]uril macrocycle as well as its stimuli-responsive breakage with adamantylamine or pH, which restores the protein structure and recognition properties. The assembly showed efficient loading and controlled release of a standard drug, doxorubicin (DOX), and the same was validated in live cells. The cell viability studies documented that the DOX-loaded assembly mask the cytotoxicity of DOX and the toxicity can be revived at the target on demand, triggering its therapeutic activation. This is found to be more effective in the cancer cells. In addition, such host-assisted protein assemblies are also highly promising for stabilizing/protecting the native protein structure, a viable approach to prevent/inhibit protein misfolding and aggregation.
authors.affiliation: Radiation & Photochemistry Division, Bhabha Atomic Research Centre, Mumbai, 400 085, India.
authors.forename: Nilotpal
authors.initials: N
authors.lastname: Barooah
authors.affiliation: Radiation & Photochemistry Division, Bhabha Atomic Research Centre, Mumbai, 400 085, India.
authors.forename: Amit
authors.initials: A
authors.lastname: Kunwar
authors.affiliation: Radiation & Photochemistry Division, Bhabha Atomic Research Centre, Mumbai, 400 085, India.|Homi Bhabha National Institute, Training School Complex, Anushaktinagar, Mumbai, 400 094, India.
authors.forename: Raman
authors.initials: R
authors.lastname: Khurana
authors.affiliation: Radiation & Photochemistry Division, Bhabha Atomic Research Centre, Mumbai, 400 085, India.|Homi Bhabha National Institute, Training School Complex, Anushaktinagar, Mumbai, 400 094, India.
authors.forename: Achikanath C
authors.initials: AC
authors.lastname: Bhasikuttan
authors.affiliation: Radiation & Photochemistry Division, Bhabha Atomic Research Centre, Mumbai, 400 085, India.|Homi Bhabha National Institute, Training School Complex, Anushaktinagar, Mumbai, 400 094, India.
authors.forename: Jyotirmayee
authors.initials: J
authors.lastname: Mohanty
mesh_terms: D000818:Animals; D000903:Antibiotics, Antineoplastic / Q000737:chemistry / Q000494:pharmacology*; D001952:Bridged-Ring Compounds / Q000737:chemistry*; D016466:CHO Cells; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D003412:Cricetulus; D004317:Doxorubicin / Q000737:chemistry / Q000494:pharmacology*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007093:Imidazoles / Q000737:chemistry*; D008856:Microscopy, Fluorescence; D049329:Nanostructures / Q000737:chemistry*; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D047028:Macrocyclic Compounds; D000099246:Cucurbit(n)urils
chemical_list: D000903:Antibiotics, Antineoplastic; D001952:Bridged-Ring Compounds; D004317:Doxorubicin; D007093:Imidazoles; D012710:Serum Albumin, Bovine; C456276:cucurbit(7)uril; D047028:Macrocyclic Compounds; D000099246:Cucurbit(n)urils
keywords: cytotoxicity; drug delivery; host-guest systems; macrocyclic host; recognition-mediated nanoassembly; supramolecular chemistry