title: Nucleo-mitochondrial interaction of yeast in response to cadmium sulfide quantum dot exposure.
abstract: Cell sensitivity to quantum dots (QDs) has been attributed to a cascade triggered by oxidative stress leading to apoptosis. The role and function of mitochondria in animal cells are well understood but little information is available on the complex genetic networks that regulate nucleo-mitochondrial interaction. The effect of CdS QD exposure in yeast Saccharomyces cerevisiae was assessed under conditions of limited lethality (<10%), using cell physiological and morphological endpoints. Whole-genomic array analysis and the screening of a deletion mutant library were also carried out. The results showed that QDs: increased the level of reactive oxygen species (ROS) and decreased the level of reduced vs oxidized glutathione (GSH/GSSG); reduced oxygen consumption and the abundance of respiratory cytochromes; disrupted mitochondrial membrane potentials and affected mitochondrial morphology. Exposure affected the capacity of cells to grow on galactose, which requires nucleo-mitochondrial involvement. However, QDs exposure did not materially induce respiratory deficient (RD) mutants but only RD phenocopies. All of these cellular changes were correlated with several key nuclear genes, including TOM5 and FKS1, involved in the maintenance of mitochondrial organization and function. The consequences of these cellular effects are discussed in terms of dysregulation of cell function in response to these "pathological mitochondria".
authors.affiliation: Department of Life Sciences, University of Parma, Parma, Italy.
authors.forename: Francesco
authors.initials: F
authors.lastname: Pasquali
authors.affiliation: Department of Life Sciences, University of Parma, Parma, Italy.
authors.forename: Caterina
authors.initials: C
authors.lastname: Agrimonti
authors.affiliation: Department of Life Sciences, University of Parma, Parma, Italy; Stockbridge school of Agriculture, University of Massachusetts, Amherst, MA, USA; The Connecticut Agricultural Experiment Station, New Haven, CT, USA.
authors.forename: Luca
authors.initials: L
authors.lastname: Pagano
authors.affiliation: IMEM-CNR - Istituto dei Materiali per l'Elettronica ed il Magnetismo, Parma, Italy.
authors.forename: Andrea
authors.initials: A
authors.lastname: Zappettini
authors.affiliation: IMEM-CNR - Istituto dei Materiali per l'Elettronica ed il Magnetismo, Parma, Italy.
authors.forename: Marco
authors.initials: M
authors.lastname: Villani
authors.affiliation: Department of Life Sciences, University of Parma, Parma, Italy.
authors.forename: Marta
authors.initials: M
authors.lastname: Marmiroli
authors.affiliation: The Connecticut Agricultural Experiment Station, New Haven, CT, USA.
authors.forename: Jason C
authors.initials: JC
authors.lastname: White
authors.affiliation: Department of Life Sciences, University of Parma, Parma, Italy; CINSA - Consorzio Interuniversitario Nazionale per le Scienze Ambientali, University of Parma, Parma, Italy. Electronic address: nelson.marmiroli@unipr.it.
authors.forename: Nelson
authors.initials: N
authors.lastname: Marmiroli
mesh_terms: D019187:Cadmium Compounds / Q000633:toxicity*; D002467:Cell Nucleus / Q000187:drug effects* / Q000235:genetics / Q000648:ultrastructure; D017353:Gene Deletion; D005800:Genes, Fungal; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D008928:Mitochondria / Q000187:drug effects* / Q000235:genetics / Q000648:ultrastructure; D018384:Oxidative Stress / Q000187:drug effects / Q000235:genetics; D045663:Quantum Dots / Q000633:toxicity*; D012441:Saccharomyces cerevisiae / Q000187:drug effects* / Q000235:genetics / Q000648:ultrastructure; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics; D013440:Sulfides / Q000633:toxicity*
chemical_list: D019187:Cadmium Compounds; D029701:Saccharomyces cerevisiae Proteins; D013440:Sulfides; C034939:cadmium sulfide
keywords: CdS quantum dots; Oxidative stress; Pathological mitochondria; Saccharomyces cerevisiae