title: Rapid bacteria identification from environmental mining samples using MALDI-TOF MS analysis.
abstract: Copper mining has polluted soils and water, causing a reduction of the microbial diversity and a change in the structure of the resident bacterial communities. In this work, selective isolation combined with MALDI-TOF MS and the 16S rDNA method were used for characterizing cultivable bacterial communities from copper mining samples. The results revealed that MALDI-TOF MS analysis can be considered a reliable and fast tool for identifying copper-resistant bacteria from environmental samples at the genera level. Even though some results were ambiguous, accuracy can be improved by enhancing reference databases. Therefore, mass spectra analysis provides a reliable method to facilitate monitoring of the microbiota from copper-polluted sites. The understanding of the microbial community diversity in copper-contaminated sites can be helpful to understand the impact of the metal on the microbiome and to design bioremediation processes.
authors.affiliation: Environmental Research and Education Center, University of São Paulo, CEPEMA-POLI-USP, Conego Domenico Rangoni Rd, 270 km, Cubatão, SP, Brazil. avanzi@usp.br.|Institute of Energy and Environment, Environmental Science Graduate Program, University of São Paulo, PROCAM-USP, Professor Luciano Gualberto St, 1289, São Paulo, SP, Brazil. avanzi@usp.br.
authors.forename: Ingrid Regina
authors.initials: IR
authors.lastname: Avanzi
authors.affiliation: Environmental Research and Education Center, University of São Paulo, CEPEMA-POLI-USP, Conego Domenico Rangoni Rd, 270 km, Cubatão, SP, Brazil.
authors.forename: Louise Hase
authors.initials: LH
authors.lastname: Gracioso
authors.affiliation: Environmental Research and Education Center, University of São Paulo, CEPEMA-POLI-USP, Conego Domenico Rangoni Rd, 270 km, Cubatão, SP, Brazil.|Chemical Engineering Department, University of São Paulo, POLI-USP, Lineu Prestes Ave, 580, São Paulo, SP, Brazil.
authors.forename: Marcela Dos Passos Galluzzi
authors.initials: MD
authors.lastname: Baltazar
authors.affiliation: Environmental Research and Education Center, University of São Paulo, CEPEMA-POLI-USP, Conego Domenico Rangoni Rd, 270 km, Cubatão, SP, Brazil.
authors.forename: Bruno
authors.initials: B
authors.lastname: Karolski
authors.affiliation: Environmental Research and Education Center, University of São Paulo, CEPEMA-POLI-USP, Conego Domenico Rangoni Rd, 270 km, Cubatão, SP, Brazil.|Department of Marine Sciences, Federal University of São Paulo, Imar-Unifesp, Alm. Saldanha da Gama Ave, 89, Santos, SP, Brazil.
authors.forename: Elen Aquino
authors.initials: EA
authors.lastname: Perpetuo
authors.affiliation: Environmental Research and Education Center, University of São Paulo, CEPEMA-POLI-USP, Conego Domenico Rangoni Rd, 270 km, Cubatão, SP, Brazil.|Institute of Energy and Environment, Environmental Science Graduate Program, University of São Paulo, PROCAM-USP, Professor Luciano Gualberto St, 1289, São Paulo, SP, Brazil.|Chemical Engineering Department, University of São Paulo, POLI-USP, Lineu Prestes Ave, 580, São Paulo, SP, Brazil.
authors.forename: Claudio Augusto Oller
authors.initials: CA
authors.lastname: do Nascimento
mesh_terms: D001419:Bacteria / Q000235:genetics / Q000302:isolation & purification*; D003300:Copper / Q000302:isolation & purification; D008906:Mining; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods; D013997:Time Factors
chemical_list: D012336:RNA, Ribosomal, 16S; D003300:Copper
keywords: Biotyper; Environmental microbiology; Heavy metals; Mass spectrometry; Metal tolerance; Species identification