title: Compact bone-derived mesenchymal stem cells attenuate nonalcoholic steatohepatitis in a mouse model by modulation of CD4 cells differentiation.
abstract: Increasing evidence has accrued which indicates that mesenchymal stem cells (MSCs) have a potential clinical value in the treatment of certain diseases. Globally, nonalcoholic steatohepatitis (NASH) is a widespread disorder. In the present study, MSCs were isolated successfully from compact bone and a mouse model of NASH was established as achieved with use of a methionine-choline deficient (MCD) diet. Compact bone-derived MSCs transplantation reduced MCD diet-induced weight loss, hepatic lipid peroxidation, steatosis, ballooning, lobular inflammation and fibrogenesis. It was shown that MSCs treatment hampered MCD diet-induced proliferation of CD4+IFN-γ+and CD4+IL-6+T spleen cells. In addition, CD4+IL-17+lymphocytes that associated with anti-inflammation show little change in MCD as well as in MCD+MSCs splenocytes. We conclude that MSCs may have a potential clinical value upon NASH, through their capacity to suppress activation of CD4+IFN-γ+and CD4+IL-6+lymphocytes.
authors.affiliation: Modern College of Arts and Science, or School of Life Science, Shanxi Normal University, Linfen, China. Electronic address: shilvshe@163.com.
authors.forename: Huafeng
authors.initials: H
authors.lastname: Wang
authors.affiliation: Central Blood Station of Tianjin, Tianjin, China.
authors.forename: Dong
authors.initials: D
authors.lastname: Wang
authors.affiliation: Modern College of Arts and Science, or School of Life Science, Shanxi Normal University, Linfen, China.
authors.forename: Luhong
authors.initials: L
authors.lastname: Yang
authors.affiliation: Modern College of Arts and Science, or School of Life Science, Shanxi Normal University, Linfen, China.
authors.forename: Yanxia
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Modern College of Arts and Science, or School of Life Science, Shanxi Normal University, Linfen, China.
authors.forename: Junli
authors.initials: J
authors.lastname: Jia
authors.affiliation: Modern College of Arts and Science, or School of Life Science, Shanxi Normal University, Linfen, China.
authors.forename: Dongchen
authors.initials: D
authors.lastname: Na
authors.affiliation: Modern College of Arts and Science, or School of Life Science, Shanxi Normal University, Linfen, China.
authors.forename: Huize
authors.initials: H
authors.lastname: Chen
authors.affiliation: Modern College of Arts and Science, or School of Life Science, Shanxi Normal University, Linfen, China.
authors.forename: Yongping
authors.initials: Y
authors.lastname: Luo
authors.affiliation: Department of Human anatomy, Shanxi Medical University, Taiyuan, China; Research Center of Basic Medical Sciences, Tianjin Medical University, Tianjin, China. Electronic address: ljjlcf@126.com.
authors.forename: Chengfang
authors.initials: C
authors.lastname: Liu
mesh_terms: D000818:Animals; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology*; D002454:Cell Differentiation; D002930:Cinchona Alkaloids; D000071538:Cortical Bone / Q000473:pathology; D004032:Diet; D004195:Disease Models, Animal; D005355:Fibrosis; D006801:Humans; D007371:Interferon-gamma / Q000378:metabolism; D015850:Interleukin-6 / Q000378:metabolism; D015227:Lipid Peroxidation; D008099:Liver / Q000473:pathology*; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000276:immunology*; D051379:Mice; D008810:Mice, Inbred C57BL; D065626:Non-alcoholic Fatty Liver Disease / Q000628:therapy*
chemical_list: D002930:Cinchona Alkaloids; D015850:Interleukin-6; C511298:N,N'-dimethylcinchoninium; D007371:Interferon-gamma
keywords: CD4(+) lymphocytes; Compact bone; MCD diet; MSCs; NASH