title: Preparation of epigallocatechin gallate-loaded nanoparticles and characterization of their inhibitory effects onHelicobacter pylorigrowthin vitroandin vivo.
abstract: A variety of approaches have been proposed for overcoming the unpleasant side effects associated with antibiotics treatment ofHelicobacter pylori(H. pylori) infections. Research has shown that epigallocatechin-3-gallate (EGCG), a major ingredient in green tea, has antibacterial activity for antiurease activity againstH. pylori. Oral EGCG is not good because of its digestive instability and the fact that it often cannot reach the targeted site of antibacterial activity. To localize EGCG toH. pyloriinfection site, this study developed a fucose-chitosan/gelatin nanoparticle to encapsulate EGCG at the target and make direct contact with the region of microorganisms on the gastric epithelium. Analysis of a simulated gastrointestinal medium indicated that the proposedin vitronanocarrier system effectively controls the release of EGCG, which interacts directly with the intercellular space at the site ofH. pyloriinfection. Meanwhile, results ofin vivoclearance assays indicated that our prepared fucose-chitosan/gelatin/EGCG nanoparticles had a significantly greaterH. pyloriclearance effect and more effectively reducedH. pylori-associated gastric inflammation in the gastric-infected mouse model than the EGCG solution alone.
authors.affiliation: Department of Biological Science and Technology, China Medical University, Taichung, Taiwan.
authors.forename: Yu-Hsin
authors.initials: YH
authors.lastname: Lin
authors.affiliation: Division of Hepatogastroenterology, Department of Internal Medicine, China Medical University Hospital, Taichung, Taiwan.
authors.forename: Chun-Lung
authors.initials: CL
authors.lastname: Feng
authors.affiliation: Department of Microbiology, School of Medicine, China Medical University, Taichung, Taiwan.
authors.forename: Chih-Ho
authors.initials: CH
authors.lastname: Lai
authors.affiliation: Bio-Medical Carbon Technology Co., Ltd, Taichung, Taiwan.
authors.forename: Jui-Hsiang
authors.initials: JH
authors.lastname: Lin
authors.affiliation: Department of Biological Science and Technology, China Medical University, Taichung, Taiwan.
authors.forename: Hao-Yun
authors.initials: HY
authors.lastname: Chen
keywords: Helicobacter pylori; epigallocatechin-3-gallate; fucose–chitosan; gelatin; nanoparticles