title: The Impact of Silver Nanoparticles Produced byBacillus pumilusAs Antimicrobial and Nematicide.
abstract: This study evaluates the potential application of silver nanoparticles (AgNPs) as antimicrobial or nematicidal agents produced by the extremophileBacillus pumilus, which was isolated from the alkaline Wadi El-Natrun Lake in Egypt. The AgNPs were characterized by ultraviolet-visible absorption spectroscopy, transmission electron microscopy, and energy dispersive x-ray spectroscopy. The size of AgNPs formed ranged from 20.12 to 29.48 nm.Panagrellus redivivuswas exposed to different concentrations (0, 50, 100, 150, and 200 μg/mL) of AgNPs in a 5 mL nematode suspension (1 × 103mL-1). The best result occurred at AgNP concentrations of 150 and 200 μg/mL, with death rates of 80 and 91%, respectively, following 48 h of exposure. AgNPs also exhibited potent antimicrobial properties when using Gram-negative and Gram-positive human pathogens, with MIC and MBC values of 5 and 10 μg/mL, respectively. These laboratory assays prove that biologically synthesized AgNPs are an ecofriendly material that can be used in lieu of solvents or toxic chemicals.
authors.affiliation: Medical Genetics Department, Faculty of Medicine, University of Jeddah Jeddah, Saudi Arabia.
authors.forename: Wael M
authors.initials: WM
authors.lastname: Mahmoud
authors.affiliation: Biology Department, Faculty of Science, University of JeddahJeddah, Saudi Arabia; Department of Agricultural Botany, Faculty of Agriculture, Suez Canal UniversityIsmailia, Egypt.
authors.forename: Tamer S
authors.initials: TS
authors.lastname: Abdelmoneim
authors.affiliation: Biology Department, Faculty of Science, University of JeddahJeddah, Saudi Arabia; Chemistry of Natural and Microbial Products Department, National Research CentreGiza, Egypt.
authors.forename: Ahmed M
authors.initials: AM
authors.lastname: Elazzazy
keywords: bactericidal; ecofriendly materials; nanomaterials; nematicidal; silver