title: A combination of resveratrol and quercetin induces browning in white adipose tissue of rats fed an obesogenic diet.
abstract: To analyze whether a combination of quercetin (Q) and resveratrol (RSV) would induce a white adipose tissue (WAT) browning effect.
  
  
  
  Thirty-six rats were fed an obesogenic diet and divided into four groups: control, treated with RSV (15 mg/kg body weight/day; RSV group), treated with Q (30 mg/kg body weight/day; Q group), or treated with both polyphenols (RSV + Q group).
  
  
  
  After 6 weeks, body and WAT weights were significantly reduced in the RSV + Q group. In perirenal WAT of the control, RSV, and Q groups, white unilocular adipocytes appeared in the majority of cells, while in the RSV + Q group numerous multilocular adipocytes with positive immunostaining for UCP1 were observed. The presence of UCP1 was confirmed by Western blot. This group also revealed increased mRNA levels of Cidea, Hocx9, Bmp4, Slc27a1, Pat2, Atgl, and Atp5d. Interscapular brown adipose tissue weight showed no differences between groups, but the Cidea mRNA level was increased in the RSV group, the Cox-2 mRNA level in the RSV + Q group, and UCP1 protein expression in the RSV and the RSV + Q groups.
  
  
  
  This study demonstrated that the RSV + Q combination produces a brown-like remodeling effect in perirenal WAT, as well as increased UCP1 protein expression in interscapular brown adipose tissue.
authors.affiliation: Department of Nutrition and Food Science, Nutrition and Obesity Group, University of the Basque Country (UPV/EHU), Lucio Lascaray Center and CIBER de Fisiopatología de la Obesidad y Nutrición (CIBERobn), Vitoria, Spain.
authors.forename: Noemí
authors.initials: N
authors.lastname: Arias
authors.affiliation: Laboratory of Molecular Biology, Nutrition and Biotechnology, University of the Balearic Islands and CIBER de Fisiopatología de la Obesidad y Nutrición (CIBERobn), Palma de Mallorca, Spain.
authors.forename: Catalina
authors.initials: C
authors.lastname: Picó
authors.affiliation: Department of Nutrition and Food Science, Nutrition and Obesity Group, University of the Basque Country (UPV/EHU), Lucio Lascaray Center and CIBER de Fisiopatología de la Obesidad y Nutrición (CIBERobn), Vitoria, Spain.
authors.forename: M
authors.initials: M
authors.lastname: Teresa Macarulla
authors.affiliation: Laboratory of Molecular Biology, Nutrition and Biotechnology, University of the Balearic Islands and CIBER de Fisiopatología de la Obesidad y Nutrición (CIBERobn), Palma de Mallorca, Spain.
authors.forename: Paula
authors.initials: P
authors.lastname: Oliver
authors.affiliation: Department of Nutrition and Food Science, Nutrition and Obesity Group, University of the Basque Country (UPV/EHU), Lucio Lascaray Center and CIBER de Fisiopatología de la Obesidad y Nutrición (CIBERobn), Vitoria, Spain.
authors.forename: Jonatan
authors.initials: J
authors.lastname: Miranda
authors.affiliation: Laboratory of Molecular Biology, Nutrition and Biotechnology, University of the Balearic Islands and CIBER de Fisiopatología de la Obesidad y Nutrición (CIBERobn), Palma de Mallorca, Spain.
authors.forename: Andreu
authors.initials: A
authors.lastname: Palou
authors.affiliation: Department of Nutrition and Food Science, Nutrition and Obesity Group, University of the Basque Country (UPV/EHU), Lucio Lascaray Center and CIBER de Fisiopatología de la Obesidad y Nutrición (CIBERobn), Vitoria, Spain.
authors.forename: María P
authors.initials: MP
authors.lastname: Portillo
mesh_terms: D017667:Adipocytes / Q000187:drug effects / Q000378:metabolism; D002001:Adipose Tissue, Brown / Q000187:drug effects / Q000502:physiology; D052436:Adipose Tissue, White / Q000187:drug effects* / Q000502:physiology; D026921:Amino Acid Transport Systems, Neutral / Q000235:genetics / Q000378:metabolism; D000818:Animals; D051017:Apoptosis Regulatory Proteins / Q000235:genetics / Q000378:metabolism; D001835:Body Weight; D055415:Bone Morphogenetic Protein 4 / Q000235:genetics / Q000378:metabolism; D051546:Cyclooxygenase 2 / Q000235:genetics / Q000378:metabolism; D004032:Diet; D050556:Fatty Acid-Binding Proteins / Q000235:genetics / Q000378:metabolism; D008049:Lipase / Q000235:genetics / Q000378:metabolism; D011794:Quercetin / Q000494:pharmacology*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D000077185:Resveratrol; D013267:Stilbenes / Q000494:pharmacology*; D027981:Symporters / Q000235:genetics / Q000378:metabolism; D000071256:Uncoupling Protein 1 / Q000235:genetics / Q000378:metabolism; D000217:Acyltransferases
chemical_list: D026921:Amino Acid Transport Systems, Neutral; D051017:Apoptosis Regulatory Proteins; D055415:Bone Morphogenetic Protein 4; D051546:Cyclooxygenase 2; D050556:Fatty Acid-Binding Proteins; D008049:Lipase; D011794:Quercetin; D012333:RNA, Messenger; D000077185:Resveratrol; D013267:Stilbenes; D027981:Symporters; D000071256:Uncoupling Protein 1; C525811:Bmp4 protein, rat; C542951:CIDEA protein, rat; C493570:Slc27a1 protein, rat; C474940:Slc36a2 protein, rat; C000604373:Ucp1 protein, rat; C489691:Ptgs2 protein, rat; C571275:PNPLA2 protein, rat; D000217:Acyltransferases