title: A Review of Spectroscopic and Biophysical-Chemical Studies of the Complex of Cyclobutane Pyrimidine Dimer Photolyase and Cryptochrome DASH with Substrate DNA.
abstract: Cyclobutane pyrimidine dimer (CPD) photolyase (PL) is a structure-specific DNA repair enzyme that uses blue light to repair CPD on DNA. Cryptochrome (CRY) DASH enzymes use blue light for the repair of CPD lesions on single-stranded (ss) DNA, although some may also repair these lesions on double-stranded (ds) DNA. In addition, CRY DASH may be involved in blue light signaling, similar to cryptochromes. The focus of this review is on spectroscopic and biophysical-chemical experiments of the enzyme-substrate complex that have contributed to a more detailed understanding of all the aspects of the CPD repair mechanism of CPD photolyase and CRY DASH. This will be performed in the backdrop of the available X-ray crystal structures of these enzymes bound to a CPD-like lesion. These structures helped to confirm conclusions that were drawn earlier from spectroscopic and biophysical-chemical experiments, and they have a critical function as a framework to design new experiments and to interpret new experimental data. This review will show the important synergy between X-ray crystallography and spectroscopic/biophysical-chemical investigations that is essential to obtain a sufficiently detailed picture of the overall mechanism of CPD photolyases and CRY DASH proteins.
authors.affiliation: Department of Chemistry and Biochemistry, Montclair State University, Montclair, NJ.
authors.forename: Johannes P M
authors.initials: JP
authors.lastname: Schelvis
authors.affiliation: Department of Chemistry and Biochemistry, Montclair State University, Montclair, NJ.
authors.forename: Yvonne M
authors.initials: YM
authors.lastname: Gindt
mesh_terms: D055592:Biophysical Phenomena; D056931:Cryptochromes / Q000737:chemistry*; D018360:Crystallography, X-Ray; D004247:DNA / Q000737:chemistry*; D004255:Deoxyribodipyrimidine Photo-Lyase / Q000737:chemistry*; D005182:Flavin-Adenine Dinucleotide / Q000737:chemistry; D015394:Molecular Structure; D011740:Pyrimidine Dimers / Q000737:chemistry*; D013057:Spectrum Analysis / Q000379:methods*; D013379:Substrate Specificity
chemical_list: D056931:Cryptochromes; D011740:Pyrimidine Dimers; D005182:Flavin-Adenine Dinucleotide; D004247:DNA; D004255:Deoxyribodipyrimidine Photo-Lyase