title: Analysis of Reparative Activity of Platelet Lysate: Effect on Cell Monolayer Recovery In Vitro and Skin Wound Healing In Vivo.
abstract: Platelet lysate prepared from donor platelet concentrate and pooled according to a developed technique stimulates migration of multipotent mesenchymal stromal cells of the human adipose tissue and promotes healing of the monolayer defect in cultures of human fibroblasts and multipotent mesenchymal stromal cells in vitro in concentrations close those of fetal calf serum (5-10%). Lysate of platelets from platelet-rich rat blood plasma stimulated healing of the skin defect by promoting epithelialization and granulation tissue formation. The regenerative properties of platelet lysate in vivo increased with increasing its concentration.
authors.affiliation: P. A. Hertsen Moscow Oncology Research Institute, Branch of the National Medical Research Radiological Centre of the Ministry of Health of the Russian Federation, Ministry of Health of the Russian Federation, Moscow, Russia. prognoz.06@mail.ru.
authors.forename: N S
authors.initials: NS
authors.lastname: Sergeeva
authors.affiliation: P. A. Hertsen Moscow Oncology Research Institute, Branch of the National Medical Research Radiological Centre of the Ministry of Health of the Russian Federation, Ministry of Health of the Russian Federation, Moscow, Russia.
authors.forename: Ya D
authors.initials: YD
authors.lastname: Shanskii
authors.affiliation: P. A. Hertsen Moscow Oncology Research Institute, Branch of the National Medical Research Radiological Centre of the Ministry of Health of the Russian Federation, Ministry of Health of the Russian Federation, Moscow, Russia.
authors.forename: I K
authors.initials: IK
authors.lastname: Sviridova
authors.affiliation: P. A. Hertsen Moscow Oncology Research Institute, Branch of the National Medical Research Radiological Centre of the Ministry of Health of the Russian Federation, Ministry of Health of the Russian Federation, Moscow, Russia.
authors.forename: P A
authors.initials: PA
authors.lastname: Karalkin
authors.affiliation: P. A. Hertsen Moscow Oncology Research Institute, Branch of the National Medical Research Radiological Centre of the Ministry of Health of the Russian Federation, Ministry of Health of the Russian Federation, Moscow, Russia.
authors.forename: V A
authors.initials: VA
authors.lastname: Kirsanova
authors.affiliation: P. A. Hertsen Moscow Oncology Research Institute, Branch of the National Medical Research Radiological Centre of the Ministry of Health of the Russian Federation, Ministry of Health of the Russian Federation, Moscow, Russia.
authors.forename: S A
authors.initials: SA
authors.lastname: Akhmedova
authors.affiliation: P. A. Hertsen Moscow Oncology Research Institute, Branch of the National Medical Research Radiological Centre of the Ministry of Health of the Russian Federation, Ministry of Health of the Russian Federation, Moscow, Russia.
authors.forename: A D
authors.initials: AD
authors.lastname: Kaprin
mesh_terms: D000818:Animals; D001681:Biological Assay; D001792:Blood Platelets / Q000737:chemistry*; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D045424:Complex Mixtures / Q000494:pharmacology*; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects*; D006801:Humans; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000187:drug effects*; D053657:Platelet-Rich Plasma / Q000737:chemistry; D061251:Primary Cell Culture; D051381:Rats; D012867:Skin / Q000187:drug effects / Q000293:injuries; D014945:Wound Healing / Q000187:drug effects*
chemical_list: D045424:Complex Mixtures
keywords: cell migration; multipotent mesenchymal stromal cells; platelet lysate; regeneration of skin defect