title: Asymmetric positioning of Cas1-2 complex and Integration Host Factor induced DNA bending guide the unidirectional homing of protospacer in CRISPR-Cas type I-E system.
abstract: CRISPR-Cas system epitomizes prokaryote-specific quintessential adaptive defense machinery that limits the genome invasion of mobile genetic elements. It confers adaptive immunity to bacteria by capturing a protospacer fragment from invading foreign DNA, which is later inserted into the leader proximal end of CRIPSR array and serves as immunological memory to recognize recurrent invasions. The universally conserved Cas1 and Cas2 form an integration complex that is known to mediate the protospacer invasion into the CRISPR array. However, the mechanism by which this protospacer fragment gets integrated in a directional fashion into the leader proximal end is elusive. Here, we employ CRISPR/dCas9 mediated immunoprecipitation and genetic analysis to identify Integration Host Factor (IHF) as an indispensable accessory factor for spacer acquisition in Escherichia coli Further, we show that the leader region abutting the first CRISPR repeat localizes IHF and Cas1-2 complex. IHF binding to the leader region induces bending by about 120° that in turn engenders the regeneration of the cognate binding site for protospacer bound Cas1-2 complex and brings it in proximity with the first CRISPR repeat. This appears to guide Cas1-2 complex to orient the protospacer invasion towards the leader-repeat junction thus driving the integration in a polarized fashion.
authors.affiliation: Department of Biosciences and Bioengineering, Indian Institute of Technology Guwahati, Guwahati 781039, Assam, India.
authors.forename: K N R
authors.initials: KN
authors.lastname: Yoganand
authors.affiliation: Department of Biosciences and Bioengineering, Indian Institute of Technology Guwahati, Guwahati 781039, Assam, India.
authors.forename: R
authors.initials: R
authors.lastname: Sivathanu
authors.affiliation: Department of Biosciences and Bioengineering, Indian Institute of Technology Guwahati, Guwahati 781039, Assam, India.
authors.forename: Siddharth
authors.initials: S
authors.lastname: Nimkar
authors.affiliation: Department of Biosciences and Bioengineering, Indian Institute of Technology Guwahati, Guwahati 781039, Assam, India banand@iitg.ernet.in.
authors.forename: B
authors.identifier: http://orcid.org/0000-0002-7125-4011
authors.initials: B
authors.lastname: Anand
mesh_terms: D001483:Base Sequence; D001665:Binding Sites; D064130:CRISPR-Associated Proteins / Q000235:genetics* / Q000276:immunology; D064113:CRISPR-Cas Systems / Q000276:immunology; D064112:Clustered Regularly Interspaced Short Palindromic Repeats; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics* / Q000276:immunology; D004706:Endodeoxyribonucleases / Q000235:genetics* / Q000276:immunology; D004720:Endonucleases / Q000235:genetics* / Q000276:immunology; D004926:Escherichia coli / Q000235:genetics* / Q000276:immunology; D029968:Escherichia coli Proteins / Q000235:genetics* / Q000276:immunology; D034982:Integration Host Factors / Q000235:genetics* / Q000276:immunology; D009690:Nucleic Acid Conformation; D011485:Protein Binding; D054730:Protein Interaction Domains and Motifs; D021382:Protein Sorting Signals / Q000235:genetics; D017433:Protein Structure, Secondary
chemical_list: D064130:CRISPR-Associated Proteins; D004269:DNA, Bacterial; D029968:Escherichia coli Proteins; D034982:Integration Host Factors; D021382:Protein Sorting Signals; C000591054:Cas2 protein, E coli; D004706:Endodeoxyribonucleases; D004720:Endonucleases; C557650:YgbT protein, E coli