title: Oharaeibacter diazotrophicus gen. nov., sp. nov., a diazotrophic and facultatively methylotrophic bacterium, isolated from rice rhizosphere.
abstract: A novel facultatively methanol-utilizing bacterial strain, SM30T, was isolated from rice rhizosphere. Strain SM30T was Gram-stain-negative, aerobic, motile, short rods, and grew optimally at pH 7 and at 28 °C. It could tolerate 0 to 2 % (w/v) NaCl. Based on 16S rRNA gene sequence comparisons, strain SM30T was most closely related to Pleomorphomonas oryzae DSM 16300T, with a low similarity of 94.17 %. One of the lanthanide metals, lanthanum, could enhance its growth slightly on methanol. Phylogenetic trees, based on the mxaF, xoxF and cpn60 genes of SM30T showed its distinct phylogenetic position with respect to species with validly published names. Polymerase chain reaction (PCR) amplification of the nifH and growth on nitrogen-free medium indicated that strain SM30T is a diazotroph. The major cellular fatty acids were summed feature 8 (containing 18 : 1ω7c and 18 : 1ω6c) and cyclo 19 : 0ω8c. The major quinone was ubiquinone 10. The DNA G+C content was 74.6 mol%. Based on the genotypic and phenotypic characteristics, strain SM30T represents a novel genus and species, for which the name Oharaeibacter diazotrophicus gen. nov., sp. nov. is proposed with the type strain SM30T (=NBRC 111955T=DSM 102969T).
authors.affiliation: Institute of Plant Science and Resources, Okayama University, Okayama, Japan.
authors.forename: Haoxin
authors.initials: H
authors.lastname: Lv
authors.affiliation: Institute of Plant Science and Resources, Okayama University, Okayama, Japan.|Advanced Low Carbon Technology Research and Development Program, Japan Science and Technology Agency, Tokyo, Japan.
authors.forename: Sachiko
authors.initials: S
authors.lastname: Masuda
authors.affiliation: Institute of Plant Science and Resources, Okayama University, Okayama, Japan.
authors.forename: Yoshiko
authors.initials: Y
authors.lastname: Fujitani
authors.affiliation: Egitim Fakultesi, Mugla Sitki Kocman University, 48170 Kotekli, Mugla, Turkey.
authors.forename: Nurettin
authors.initials: N
authors.lastname: Sahin
authors.affiliation: Institute of Plant Science and Resources, Okayama University, Okayama, Japan.
authors.forename: Akio
authors.initials: A
authors.lastname: Tani
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D007564:Japan; D042083:Methylocystaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D012275:Oryza / Q000382:microbiology*; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D058441:Rhizosphere; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D014451:Ubiquinone / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D014451:Ubiquinone; C025204:Ubiquinone Q2