title: Nuclear dynamics during ascospore germination in Sordaria macrospora.
abstract: The ascomycete Sordaria macrospora has a long history as a model organism for studying fungal sexual development. Starting from an ascospore, sexual fruiting bodies (perithecia) develop within seven days and discharge new ascospores. Sexual development has been studied in detail, revealing genes required for perithecium formation and ascospore germination. However, the germination process per se has not yet been examined. Here I analyze nuclear dynamics during ascospore germination using a fluorescently labeled histone. Live-cell imaging revealed that nuclei are transported into germination vesicles that form on one side of the spore. Polar growth is established from these vesicles.
authors.affiliation: Lehrstuhl für Allgemeine und Molekulare Botanik, Ruhr-Universitaet Bochum, Universitaetsstrasse 150, 44780 Bochum, Germany. Electronic address: ines.teichert@rub.de.
authors.forename: Ines
authors.initials: I
authors.lastname: Teichert
mesh_terms: D048690:Fruiting Bodies, Fungal; D005656:Fungal Proteins / Q000235:genetics* / Q000302:isolation & purification; D018525:Germination / Q000235:genetics; D006657:Histones / Q000737:chemistry / Q000235:genetics; D025301:Hyphae / Q000235:genetics* / Q000254:growth & development; D009154:Mutation; D061848:Optical Imaging; D020037:Sordariales / Q000235:genetics* / Q000254:growth & development; D013172:Spores, Fungal / Q000235:genetics* / Q000254:growth & development
chemical_list: D005656:Fungal Proteins; D006657:Histones
keywords: Ascospore germination; Live-cell imaging; Sordaria macrospora