title: RNCR3: A regulator of diabetes mellitus-related retinal microvascular dysfunction.
abstract: Retinal microvascular abnormality is an important pathological feature of diabetic retinopathy. Herein, we report the role of lncRNA-RNCR3 in diabetes mellitus-induced retinal microvascular abnormalities. We show that RNCR3 is significantly up-regulated upon high glucose stress in vivo and in vitro. RNCR3 knockdown alleviates retinal vascular dysfunction in vivo, as shown by decreased acellular capillaries, decreased vascular leakage, and reduced inflammatory response. RNCR3 knockdown decreases retinal endothelial cell proliferation, and reduces cell migration and tube formation in vitro. RNCR3 regulates endothelial cell function through RNCR3/KLF2/miR-185-5p regulatory network. RNCR3 inhibition may be a treatment option for the prevention of diabetes mellitus-induced retinal microvascular abnormalities.
authors.affiliation: Research Center, Eye & ENT Hospital, Shanghai Medical College, Fudan University, Shanghai, China; Shanghai Key Laboratory of Visual Impairment and Restoration, Shanghai, China; The Fourth School of Clinical Medicine, Nanjing Medical University, Nanjing, China.
authors.forename: Kun
authors.initials: K
authors.lastname: Shan
authors.affiliation: Huai' an First People's Hospital, Nanjing Medical University, Huai an, Jiangsu, China.
authors.forename: Chao-Peng
authors.initials: CP
authors.lastname: Li
authors.affiliation: Research Center, Eye & ENT Hospital, Shanghai Medical College, Fudan University, Shanghai, China; Shanghai Key Laboratory of Visual Impairment and Restoration, Shanghai, China; The Fourth School of Clinical Medicine, Nanjing Medical University, Nanjing, China.
authors.forename: Chang
authors.initials: C
authors.lastname: Liu
authors.affiliation: Research Center, Eye & ENT Hospital, Shanghai Medical College, Fudan University, Shanghai, China; Shanghai Key Laboratory of Visual Impairment and Restoration, Shanghai, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Liu
authors.affiliation: Research Center, Eye & ENT Hospital, Shanghai Medical College, Fudan University, Shanghai, China; Shanghai Key Laboratory of Visual Impairment and Restoration, Shanghai, China. Electronic address: biao.yan@fdeent.org.
authors.forename: Biao
authors.initials: B
authors.lastname: Yan
mesh_terms: D000818:Animals; D049109:Cell Proliferation; D048909:Diabetes Complications / Q000378:metabolism*; D003930:Diabetic Retinopathy / Q000378:metabolism*; D042783:Endothelial Cells / Q000378:metabolism; D020868:Gene Silencing; D005947:Glucose / Q000737:chemistry; D007249:Inflammation; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D008833:Microcirculation; D009474:Neurons / Q000378:metabolism; D062085:RNA, Long Noncoding / Q000235:genetics / Q000378:metabolism; D012160:Retina / Q000378:metabolism
chemical_list: D035683:MicroRNAs; C540079:Mirn124 microRNA, mouse; D062085:RNA, Long Noncoding; D005947:Glucose
keywords: Diabetic retinopathy; Long noncoding RNA; Microvascular abnormalities