title: PEG3 Interacts with KAP1 through KRAB-A.
abstract: Peg3 (Paternally Expressed Gene 3) is an imprinted gene that encodes a zinc finger DNA-binding protein. Peg3 itself is localized in the middle of a KRAB-A (Kruppel-Associated Box) zinc finger gene cluster. The amino acid sequence encoded by its exon 7 also shows sequence similarity to that of KRAB-A, suggesting Peg3 as a KRAB-containing zinc finger gene. As predicted, the PEG3 protein was co-immunoprecipitated with KAP1, a co-repressor that interacts with KRAB-A. A series of follow-up experiments further demonstrated that the exon 7 of PEG3 is indeed responsible for its physical interaction with KAP1. ChIP and promoter assays also indicated that PEG3 likely controls its downstream genes through the KAP1-mediated repression mechanism. Overall, the current study identifies PEG3 as a KRAB-containing zinc finger protein that interacts with the co-repressor protein KAP1.
authors.affiliation: Department of Biological Sciences, Louisiana State University, Baton Rouge, Louisiana, United States of America.
authors.forename: Hongzhi
authors.initials: H
authors.lastname: He
authors.affiliation: Department of Biological Sciences, Louisiana State University, Baton Rouge, Louisiana, United States of America.
authors.forename: An
authors.initials: A
authors.lastname: Ye
authors.affiliation: Department of Biological Sciences, Louisiana State University, Baton Rouge, Louisiana, United States of America.
authors.forename: Hana
authors.initials: H
authors.lastname: Kim
authors.affiliation: Department of Biological Sciences, Louisiana State University, Baton Rouge, Louisiana, United States of America.
authors.forename: Joomyeong
authors.initials: J
authors.lastname: Kim
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002478:Cells, Cultured; D005091:Exons; D017930:Genes, Reporter; D006657:Histones / Q000378:metabolism; D006801:Humans; D047468:Immunoprecipitation; D051741:Kruppel-Like Transcription Factors / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D008969:Molecular Sequence Data; D005810:Multigene Family; D010957:Plasmids / Q000235:genetics / Q000378:metabolism; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D000072417:Protein Domains; D012097:Repressor Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D016415:Sequence Alignment; D000076183:Tripartite Motif-Containing Protein 28; D016335:Zinc Fingers / Q000235:genetics
chemical_list: D006657:Histones; D051741:Kruppel-Like Transcription Factors; C490820:PEG3 protein, human; D012097:Repressor Proteins; C422407:ZNF350 protein, human; C101116:TRIM28 protein, human; D000076183:Tripartite Motif-Containing Protein 28