title: Chromatin binding of Gcn5 in Drosophila is largely mediated by CP190.
abstract: Centrosomal 190 kDa protein (CP190) is a promoter binding factor, mediates long-range interactions in the context of enhancer-promoter contacts and in chromosomal domain formation. All Drosophila insulator proteins bind CP190 suggesting a crucial role in insulator function. CP190 has major effects on chromatin, such as depletion of nucleosomes, high nucleosomal turnover and prevention of heterochromatin expansion. Here, we searched for enzymes, which might be involved in CP190 mediated chromatin changes. Eighty percent of the genomic binding sites of the histone acetyltransferase Gcn5 are colocalizing with CP190 binding. Depletion of CP190 reduces Gcn5 binding to chromatin. Binding dependency was further supported by Gcn5 mediated co-precipitation of CP190. Gcn5 is known to activate transcription by histone acetylation. We used the dCas9 system to target CP190 or Gcn5 to a Polycomb repressed and H3K27me3 marked gene locus. Both, CP190 as well as Gcn5, activate this locus, thus supporting the model that CP190 recruits Gcn5 and thereby activates chromatin.
authors.affiliation: Institute for Genetics, Justus-Liebig-University, D35392 Giessen, Germany.
authors.forename: Tamer
authors.initials: T
authors.lastname: Ali
authors.affiliation: CECAD Research Center, University of Cologne, D50931 Cologne, Germany.
authors.forename: Marcus
authors.initials: M
authors.lastname: Krüger
authors.affiliation: Helmholtz Centre for Infection Research, D38124 Braunschweig, Germany.
authors.forename: Sabin
authors.initials: S
authors.lastname: Bhuju
authors.affiliation: Helmholtz Centre for Infection Research, D38124 Braunschweig, Germany.
authors.forename: Michael
authors.initials: M
authors.lastname: Jarek
authors.affiliation: Institute for Genetics, Justus-Liebig-University, D35392 Giessen, Germany.
authors.forename: Marek
authors.initials: M
authors.lastname: Bartkuhn
authors.affiliation: Institute for Genetics, Justus-Liebig-University, D35392 Giessen, Germany.
authors.forename: Rainer
authors.initials: R
authors.lastname: Renkawitz
mesh_terms: D000818:Animals; D001665:Binding Sites; D002478:Cells, Cultured; D002843:Chromatin / Q000378:metabolism*; D004330:Drosophila / Q000235:genetics / Q000378:metabolism; D029721:Drosophila Proteins / Q000378:metabolism*; D051548:Histone Acetyltransferases / Q000378:metabolism*; D008869:Microtubule-Associated Proteins / Q000378:metabolism*; D009687:Nuclear Proteins / Q000378:metabolism*; D015533:Transcriptional Activation; D000099224:p300-CBP-Associated Factor
chemical_list: D002843:Chromatin; D029721:Drosophila Proteins; D051548:Histone Acetyltransferases; D008869:Microtubule-Associated Proteins; D009687:Nuclear Proteins; C107964:CP190 protein, Drosophila; C470557:GCN5 protein, Drosophila; D000099224:p300-CBP-Associated Factor