title: Eicosapentaenoic acid triggers Ca2+release and Ca2+influx in mouse cerebral cortex endothelial bEND.3 cells.
abstract: Eicosapentaenoic acid (EPA), an omega-3 fatty acid abundant in fish oil, protects endothelial cells (EC) from lipotoxicity and triggers EC NO release. The latter is related to an elevation of cytosolic Ca2+. Although EPA has been shown to cause human EC cytosolic Ca2+elevation, the mechanism is unclear. Microfluorimetric imaging was used here to measure free cytosolic Ca2+concentration. EPA was shown to cause intracellular Ca2+release in mouse cerebral cortex endothelial bEND.3 cells; interestingly, the EPA-sensitive intracellular Ca2+pool(s) appeared to encompass and was larger than the Ca2+pool mobilized by sarcoplasmic-endoplasmic reticulum Ca2+-ATPase inhibition by cyclopiazonic acid. EPA also opened a Ca2+influx pathway pharmacologically distinct from store-operated Ca2+influx. Surprisingly, EPA-triggered Ca2+influx was Ni2+-insensitive; and EPA did not trigger Mn2+influx. Further, EPA-triggered Ca2+influx did not involve Na+-Ca2+exchangers. Thus, our results suggest EPA triggered unusual mechanisms of Ca2+release and Ca2+influx in EC.
authors.affiliation: Department of Anesthesiology, Chang Gung Memorial Hospital, Chiayi, Taiwan.|Chang Gung University of Science and Technology, Chiayi, Taiwan.
authors.forename: King-Chuen
authors.initials: KC
authors.lastname: Wu
authors.affiliation: Department of Anesthesiology, China Medical University Hospital, Taichung, Taiwan.
authors.forename: Kar-Lok
authors.initials: KL
authors.lastname: Wong
authors.affiliation: Department of Physiology, China Medical University, Taichung, Taiwan.
authors.forename: Mei-Ling
authors.initials: ML
authors.lastname: Wang
authors.affiliation: Department of Physiology, China Medical University, Taichung, Taiwan.
authors.forename: Lian-Ru
authors.initials: LR
authors.lastname: Shiao
authors.affiliation: Division of Cardiology, Department of Internal Medicine, Kiang Wu Hospital, Macau, China.
authors.forename: Iat-Lon
authors.initials: IL
authors.lastname: Leong
authors.affiliation: Department of Physiology, China Medical University, Taichung, Taiwan.
authors.forename: Chi-Li
authors.initials: CL
authors.lastname: Gong
authors.affiliation: Department of Anesthesiology, China Medical University Hospital, Taichung, Taiwan.|Department of Anesthesiology, The Qingdao University Yuhuangding Hospital, Yantai, Shandong, China.
authors.forename: Ka-Shun
authors.initials: KS
authors.lastname: Cheng
authors.affiliation: Division of Cardiology, Department of Medicine, Taipei Medical University Wan Fang Hospital, Taipei, Taiwan.
authors.forename: Paul
authors.initials: P
authors.lastname: Chan
authors.affiliation: Department of Physiology, China Medical University, Taichung, Taiwan. ymleung@mail.cmu.edu.tw.
authors.forename: Yuk-Man
authors.initials: YM
authors.lastname: Leung
mesh_terms: D000251:Adenosine Triphosphatases / Q000378:metabolism; D000818:Animals; D002118:Calcium / Q000378:metabolism*; D015220:Calcium Channels / Q000378:metabolism*; D002460:Cell Line; D002540:Cerebral Cortex / Q000187:drug effects* / Q000378:metabolism; D015118:Eicosapentaenoic Acid / Q000494:pharmacology*; D042783:Endothelial Cells / Q000187:drug effects* / Q000378:metabolism; D007211:Indoles / Q000494:pharmacology; D017136:Ion Transport / Q000187:drug effects; D051379:Mice
chemical_list: D015220:Calcium Channels; D007211:Indoles; D015118:Eicosapentaenoic Acid; D000251:Adenosine Triphosphatases; D002118:Calcium; C000543:cyclopiazonic acid
keywords: Ca2+ influx; Ca2+ release; Eicosapentaenoic acid; Endothelial cells