title: Two novel mutations in PRPF3 causing autosomal dominant retinitis pigmentosa.
abstract: Retinitis pigmentosa (RP) is a heterogeneous set of hereditary eye diseases, characterized by selective death of photoreceptor cells in the retina, resulting in progressive visual impairment. Approximately 20-40% of RP cases are autosomal dominant RP (ADRP). In this study, a Chinese ADRP family previously localized to the region between D1S2819 and D1S2635 was sequenced via whole-exome sequencing and a variant c.1345C > G (p.R449G) was identified in PRPF3. The Sanger sequencing was performed in probands of additional 95 Chinese ADRP families to investigate the contribution of PRPF3 to ADRP in Chinese population and another variant c.1532A > C (p.H511P) was detected in one family. These two variants, co-segregate with RP in two families respectively and both variants are predicted to be pathological. This is the first report about the spectrum of PRPF3 mutations in Chinese population, leading to the identification of two novel PRPF3 mutations. Only three clustered mutations in PRPF3 have been identified so far in several populations and all are in exon 11. Our study expands the spectrum of PRPF3 mutations in RP. We also demonstrate that PRPF3 mutations are responsible for 2.08% of ADRP families in this cohort indicating that PRPF3 mutations might be relatively rare in Chinese ADRP patients.
authors.affiliation: Department of Ophthalmology of Shanghai Tenth People's Hospital, and Tongji Eye Institute, Tongji University School of Medicine, Shanghai, China.|Department of Medical Genetics, Tongji University School of Medicine, Shanghai, China.
authors.forename: Zilin
authors.initials: Z
authors.lastname: Zhong
authors.affiliation: Center for Gene Diagnosis, Zhongnan Hospital of Wuhan University, Wuhan, China.
authors.forename: Ming
authors.initials: M
authors.lastname: Yan
authors.affiliation: Department of Ophthalmology of Shanghai Tenth People's Hospital, and Tongji Eye Institute, Tongji University School of Medicine, Shanghai, China.|Department of Medical Genetics, Tongji University School of Medicine, Shanghai, China.
authors.forename: Wan
authors.initials: W
authors.lastname: Sun
authors.affiliation: Department of Ophthalmology of Shanghai Tenth People's Hospital, and Tongji Eye Institute, Tongji University School of Medicine, Shanghai, China.|Department of Medical Genetics, Tongji University School of Medicine, Shanghai, China.
authors.forename: Zehua
authors.initials: Z
authors.lastname: Wu
authors.affiliation: Department of Ophthalmology of Shanghai Tenth People's Hospital, and Tongji Eye Institute, Tongji University School of Medicine, Shanghai, China.|Department of Medical Genetics, Tongji University School of Medicine, Shanghai, China.
authors.forename: Liyun
authors.initials: L
authors.lastname: Han
authors.affiliation: National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing, China.
authors.forename: Zheng
authors.initials: Z
authors.lastname: Zhou
authors.affiliation: Center for Gene Diagnosis, Zhongnan Hospital of Wuhan University, Wuhan, China.
authors.forename: Fang
authors.initials: F
authors.lastname: Zheng
authors.affiliation: Department of Ophthalmology of Shanghai Tenth People's Hospital, and Tongji Eye Institute, Tongji University School of Medicine, Shanghai, China.|Department of Medical Genetics, Tongji University School of Medicine, Shanghai, China.
authors.forename: Jianjun
authors.initials: J
authors.lastname: Chen
mesh_terms: D000293:Adolescent; D044466:Asian People / Q000235:genetics*; D002648:Child; D002675:Child, Preschool; D005260:Female; D020022:Genetic Predisposition to Disease; D006801:Humans; D008297:Male; D008958:Models, Molecular; D009154:Mutation; D009687:Nuclear Proteins / Q000737:chemistry / Q000235:genetics*; D010375:Pedigree; D020641:Polymorphism, Single Nucleotide; D011487:Protein Conformation; D012174:Retinitis Pigmentosa / Q000235:genetics*; D017414:Ribonucleoprotein, U4-U6 Small Nuclear / Q000737:chemistry / Q000235:genetics*; D000073359:Exome Sequencing; D055815:Young Adult
chemical_list: D009687:Nuclear Proteins; C108507:PRPF3 protein, human; D017414:Ribonucleoprotein, U4-U6 Small Nuclear