title: TRIM11, a direct target of miR-24-3p, promotes cell proliferation and inhibits apoptosis in colon cancer.
abstract: TRIM11 (tripartite motif-containing protein 11) is an E3 ubiquitin ligase recently identified as an oncogene in malignant glioma and lung cancer. In the present study, we report that expression of TRIM11 was increased in colon cancer (CC) tissue relative to paired normal tissues and that higher TRIM11 levels predicted poor overall survival (OS) and disease-free survival (DFS) in CC patients. Mechanistically, we showed that miR-24-3p downregulation contributes to TRIM11 upregulation in CC. We also demonstrated that TRIM11 overexpression promotes cell proliferation and colony formation and inhibits apoptosis in CC, while knocking down TRIM11 using CRISPR/Cas9-mediated genome editing inhibited cell proliferation and induced apoptosis. Silencing TRIM11 in vivo decreased tumor growth. These findings indicate that TRIM11 facilitates CC progression by promoting cell proliferation and inhibiting apoptosis and that the novel miR-24-3p/TRIM11 axis may be a useful new target for treating patients with CC.
authors.affiliation: Department of Pharmacy, Jiangxi Cancer Hospital, Nanchang, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Yin
authors.affiliation: Department of Radiotherapy, Jiangxi Cancer Hospital, Nanchang, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Zhong
authors.affiliation: Department of Radiation Oncology, The Affiliated Hospital of Guilin Medical University, Guilin, China.
authors.forename: Si-Wei
authors.initials: SW
authors.lastname: Li
authors.affiliation: Department of Pharmacy, Ruikang Hospital, Guangxi University of Chinese Medicine, Nanning, China.
authors.forename: Jian-Zhe
authors.initials: JZ
authors.lastname: Li
authors.affiliation: Department of Pharmacy, Jiangxi Cancer Hospital, Nanchang, China.
authors.forename: Min
authors.initials: M
authors.lastname: Zhou
authors.affiliation: Department of Pharmacy, Jiangxi Cancer Hospital, Nanchang, China.
authors.forename: Yin
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Nanchang Key Laboratory of Cancer Pathogenesis and Translational Research, Center Laboratory, The Third Affiliated Hospital, Nanchang University, Nanchang, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Sang
authors.affiliation: Department of Pharmacy, Jiangxi Cancer Hospital, Nanchang, China.
authors.forename: Lijuan
authors.initials: L
authors.lastname: Liu
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000235:genetics*; D064113:CRISPR-Cas Systems; D018938:Caco-2 Cells; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000235:genetics*; D003110:Colonic Neoplasms / Q000235:genetics* / Q000473:pathology; D015972:Gene Expression Regulation, Neoplastic; D045325:HCT116 Cells; D019073:HT29 Cells; D006801:Humans; D008819:Mice, Nude; D035683:MicroRNAs / Q000235:genetics*; D000070584:Tripartite Motif Proteins / Q000235:genetics*; D047368:Tumor Burden / Q000235:genetics; D044767:Ubiquitin-Protein Ligases / Q000235:genetics*; D023041:Xenograft Model Antitumor Assays / Q000379:methods
chemical_list: C543845:MIRN24 microRNA, human; D035683:MicroRNAs; D000070584:Tripartite Motif Proteins; C510874:TRIM11 protein, human; D044767:Ubiquitin-Protein Ligases
keywords: TRIM11; colon cancer; miR-24-3p