title: microRNA-145 regulates the RLR signaling pathway in miiuy croaker after poly(I:C) stimulation via targeting MDA5.
abstract: MicroRNAs (miRNAs) are endogenous small non-coding RNAs that participate in diverse biological processes via degrading the target mRNAs or repressing translation. In this study, the regulation of miRNA to the RLR (RIG-I-like receptor) signaling pathway by degrading the target mRNAs was researched in miiuy croaker. MDA5, a microRNA-145-5p (miR-145-5p) putative target gene, was predicted by bioinformatics, and the target sites from the 3'untranslated region of MDA5 transcripts were confirmed using luciferase reporter assays. Pre-miR-145 was more effective in inhibiting MDA5 than miR-145-5p mimic, and the effect was dose- and time-dependent. The expression patterns of miR-145-5p and MDA5 were analyzed in liver and kidney from miiuy croaker. Results implied that miR-145-5p may function via degrading the MDA5 mRNAs, thereby regulating the RLR signaling pathway. Studies on miR-145-5p will enrich knowledge of its functions in immune response regulation in fish, as well as offer a basis for regulatory networks that are composed of numerous miRNAs.
authors.affiliation: Laboratory of Fish Biogenetics & Immune Evolution, College of Marine Science, Zhejiang Ocean University, Zhoushan, 316022, China.
authors.forename: Jingjing
authors.initials: J
authors.lastname: Han
authors.affiliation: Laboratory of Fish Biogenetics & Immune Evolution, College of Marine Science, Zhejiang Ocean University, Zhoushan, 316022, China.
authors.forename: Yuena
authors.initials: Y
authors.lastname: Sun
authors.affiliation: Laboratory of Fish Biogenetics & Immune Evolution, College of Marine Science, Zhejiang Ocean University, Zhoushan, 316022, China.
authors.forename: Weihua
authors.initials: W
authors.lastname: Song
authors.affiliation: Laboratory of Fish Biogenetics & Immune Evolution, College of Marine Science, Zhejiang Ocean University, Zhoushan, 316022, China. Electronic address: tianjunxu@163.com.
authors.forename: Tianjun
authors.initials: T
authors.lastname: Xu
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D019295:Computational Biology; D003198:Computer Simulation; D000071457:DEAD Box Protein 58 / Q000235:genetics / Q000378:metabolism*; D053487:DEAD-box RNA Helicases / Q000235:genetics / Q000378:metabolism*; D015870:Gene Expression; D053263:Gene Regulatory Networks / Q000235:genetics*; D007668:Kidney / Q000378:metabolism*; D008099:Liver / Q000378:metabolism*; D035683:MicroRNAs / Q000235:genetics*; D010473:Perciformes / Q000276:immunology*; D011070:Poly I-C / Q000276:immunology; D015398:Signal Transduction; D029961:Zebrafish Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: C543499:MIRN145a microRNA, mouse; D035683:MicroRNAs; D029961:Zebrafish Proteins; C586913:MDA5 protein,zebrafish; D000071457:DEAD Box Protein 58; D053487:DEAD-box RNA Helicases; D011070:Poly I-C
keywords: MDA5; MicroRNAs; Negatively regulate; RLRs; microRNA-145