title: Targeted chimera delivery to ovarian cancer cells by heterogeneous gold magnetic nanoparticle.
abstract: Efficient delivery of small interfering RNAs (siRNAs) to the targeted cells has remained a significant challenge in clinical applications. In the present study, we developed a novel aptamer-siRNA chimera delivery system mediated by cationic Au-Fe3O4nanoparticles (NPs). The chimera constructed by VEGF RNA aptamer and Notch3 siRNA was bonded with heterogeneous Au-Fe3O4nanoparticles by electrostatic interaction. The obtained complex exhibited much higher silencing efficiency against Notch3 gene compared with chimera alone and lipofectamine-siRNA complex, and improved the antitumor effects of the loaded chimera. Moreover, the efficient delivery of the chimera by Au-Fe3O4NPs could reverse multi-drug resistance (MDR) of ovarian cancer cells against the chemotherapeutic drug cisplatin, indicating its potential capability for future targeted cancer therapy while overcoming MDR.
authors.affiliation: Department of Obstetrics and Gynaecology, Tongji Hospital of Tongji University, Tongji University School of Medicine, Shanghai 200065, People's Republic of China. Second Affiliated Hospital of Nanjing Medical University, 121 Jiangjiayuan Road, Nanjing 210011, People's Republic of China.
authors.forename: Yao
authors.initials: Y
authors.lastname: Chen
authors.forename: Mengjiao
authors.initials: M
authors.lastname: Xu
authors.forename: Yi
authors.initials: Y
authors.lastname: Guo
authors.forename: Keyao
authors.initials: K
authors.lastname: Tu
authors.forename: Weimin
authors.initials: W
authors.lastname: Wu
authors.forename: Jianjun
authors.initials: J
authors.lastname: Wang
authors.forename: Xiaowen
authors.initials: X
authors.lastname: Tong
authors.forename: Wenjuan
authors.initials: W
authors.lastname: Wu
authors.forename: Lifeng
authors.initials: L
authors.lastname: Qi
authors.forename: Donglu
authors.initials: D
authors.lastname: Shi
mesh_terms: D052157:Aptamers, Nucleotide / Q000737:chemistry / Q000494:pharmacology; D045744:Cell Line, Tumor; D016503:Drug Delivery Systems / Q000379:methods*; D018432:Drug Resistance, Multiple / Q000187:drug effects*; D019008:Drug Resistance, Neoplasm / Q000187:drug effects*; D005260:Female; D006046:Gold / Q000737:chemistry*; D006801:Humans; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D009363:Neoplasm Proteins / Q000037:antagonists & inhibitors* / Q000096:biosynthesis; D010051:Ovarian Neoplasms; D042461:Vascular Endothelial Growth Factor A / Q000037:antagonists & inhibitors* / Q000096:biosynthesis
chemical_list: D052157:Aptamers, Nucleotide; D058185:Magnetite Nanoparticles; D009363:Neoplasm Proteins; C467484:VEGFA protein, human; D042461:Vascular Endothelial Growth Factor A; D006046:Gold