title: Betulin inhibited cigarette smoke-induced COPD in mice.
abstract: The purpose of the present study was to evaluate the protective effect of betulin (BE) on CS (cigarette smoke)-induced COPD in mice and explore its underlying mechanisms. 60 male ICR mice were randomly assigned to five groups: control group, model group, dexamethasone (2mg/kg) group, BE (20mg/kg) group and BE (40mg/kg) group. The COPD mice were induced by cigarette smoke exposure for 8 weeks. The result of H&E staining demonstrated that BE inhibited CS-induced pathological injury in lung tissue. Besides, BE could restore the activities of superoxide dismutase (SOD) in serum and in lung, catalase (CAT) in serum and reduce the content of malondialdehyde (MDA) in serum and in lung. BE also inhibited the overproductions of pro-inflammatory cytokines including tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6) and interleukin-1β (IL-1β). Furthermore, the administration of BE significantly inhibited the protein expression of ROCK/NF-κB pathway in CS-induced mice. Our findings suggested that BE might effectively ameliorate the progression of COPD via ROCK/NF-κB pathway in mice.
authors.affiliation: Central Laboratory, Nanjing Municipal Hospital of T.C.M, The Third Affiliated Hospital of Nanjing University of T.C.M, Nanjing 210001,China.
authors.forename: Ma
authors.initials: M
authors.lastname: Chunhua
authors.affiliation: Central Laboratory, Nanjing Municipal Hospital of T.C.M, The Third Affiliated Hospital of Nanjing University of T.C.M, Nanjing 210001,China. Electronic address: longhongyantcm@163.com.
authors.forename: Hongyan
authors.initials: H
authors.lastname: Long
authors.affiliation: Central Laboratory, Nanjing Municipal Hospital of T.C.M, The Third Affiliated Hospital of Nanjing University of T.C.M, Nanjing 210001,China.
authors.forename: Weina
authors.initials: W
authors.lastname: Zhu
authors.affiliation: Central Laboratory, Nanjing Municipal Hospital of T.C.M, The Third Affiliated Hospital of Nanjing University of T.C.M, Nanjing 210001,China.
authors.forename: Zheng
authors.initials: Z
authors.lastname: Liu
authors.affiliation: Central Laboratory, Nanjing Municipal Hospital of T.C.M, The Third Affiliated Hospital of Nanjing University of T.C.M, Nanjing 210001,China.
authors.forename: Ruan
authors.initials: R
authors.lastname: Jie
authors.affiliation: Central Laboratory, Nanjing Municipal Hospital of T.C.M, The Third Affiliated Hospital of Nanjing University of T.C.M, Nanjing 210001,China.
authors.forename: Yajie
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Central Laboratory, Nanjing Municipal Hospital of T.C.M, The Third Affiliated Hospital of Nanjing University of T.C.M, Nanjing 210001,China.
authors.forename: Yarui
authors.initials: Y
authors.lastname: Wang
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000008:administration & dosage / Q000494:pharmacology; D000975:Antioxidants / Q000378:metabolism; D016207:Cytokines / Q000235:genetics / Q000378:metabolism; D003907:Dexamethasone / Q000008:administration & dosage / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D005786:Gene Expression Regulation / Q000187:drug effects; D015227:Lipid Peroxidation; D008168:Lung / Q000473:pathology; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D029424:Pulmonary Disease, Chronic Obstructive / Q000517:prevention & control*; D011897:Random Allocation; D012906:Smoke / Q000009:adverse effects*; D014028:Tobacco Smoke Pollution / Q000009:adverse effects; D014315:Triterpenes / Q000008:administration & dosage / Q000494:pharmacology*; D000094062:Betulinic Acid
chemical_list: D000893:Anti-Inflammatory Agents; D000975:Antioxidants; D016207:Cytokines; D003907:Dexamethasone; D012906:Smoke; D014028:Tobacco Smoke Pollution; D014315:Triterpenes; C002503:betulin; D000094062:Betulinic Acid
keywords: Betulin; COPD; ROCK/NF-κB