title: Clinico-pathology and hemato-biochemistry responses in buffaloes infected with Pasteurella multocida type B:2 immunogen outer membrane protein.
abstract: The aim of this study was to investigate the clinico-pathology and haemato-biochemistry alterations in buffaloes inoculated with Pasteurella multocida type B:2 immunogen outer membrane protein via subcutaneous and oral routes. Nine buffalo heifers were divided equally into 3 treatment groups. Group 1 was inoculated orally with 10 mL of phosphate buffer saline (PBS); Group 2 and 3 were inoculated with 10 mL of outer membrane protein broth subcutaneously and orally respectively. Group 2 buffaloes showed typical haemorrhagic septicaemia clinical signs and were only able to survive for 72 h of the experiment. However, Group 3 buffaloes were able to survive throughout the stipulated time of 21 days of experiment. There were significant differences (p < 0.05) in the rectal temperature between the experimental and control group. In the hematology and biochemistry findings, there were significant differences (p < 0.05) in packed cell volume, mean corpuscular volume, mean corpuscular haemoglobin concentration, leukocytes, band neutrophils, segmented neutrophils, lymphocytes, eosinophils, basophils, gamma glutamyl transferase, total protein, and globulin between Group 2 and control group. In contrast, Group 3 and control group revealed significant differences (p < 0.05) in erythrocytes, haemoglobin, mean corpuscular haemoglobin concentration, segmented neutrophils, lymphocytes, monocytes, eosinophils, basophils, thrombocytes, gamma glutamyl transferase, total protein, globulin, and albumin:globulin ratio. In Group 2 buffaloes, there were gross lesions observed in the lung, trachea, heart, liver, spleen, kidney and submandibulae lymph nodes. In contrast, lesions were only observed in the lung, and liver of Group 3 buffaloes. There were significant differences (p < 0.05) in hemorrhage and congestion; necrosis and degeneration; and inflammatory cells infiltration between experimental groups and control group. However, there were no significant differences (p > 0.05) in edema between groups except for the lung. This study was a proof that oral route infection of Pasteurella multocida type B:2 immunogen outer membrane protein can be used to stimulate host cell.
authors.affiliation: Department of Veterinary Clinical Studies, Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia.
authors.forename: Eric Lim Teik
authors.initials: ELT
authors.lastname: Chung
authors.affiliation: Department of Veterinary Clinical Studies, Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia. Electronic address: jesseariasamy@gmail.com.
authors.forename: Faez Firdaus Jesse
authors.initials: FFJ
authors.lastname: Abdullah
authors.affiliation: Department of Veterinary Clinical Studies, Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia; Department of Veterinary Internal Medicine, Faculty of Veterinary Medicine, Al-Qasim Green University, Iraq.
authors.forename: Ali Dhiaa
authors.initials: AD
authors.lastname: Marza
authors.affiliation: Department of Veterinary Clinical Studies, Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia.
authors.forename: Wessam Monther Mohammed
authors.initials: WMM
authors.lastname: Saleh
authors.affiliation: Department of Veterinary Clinical Studies, Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia; Technical Institute Babil, Al Furat Alawast Technical University, Iraq.
authors.forename: Hayder Hamzah
authors.initials: HH
authors.lastname: Ibrahim
authors.affiliation: Department of Veterinary Pathology and Microbiology, Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia.
authors.forename: Yusuf
authors.initials: Y
authors.lastname: Abba
authors.affiliation: Department of Veterinary Pathology and Microbiology, Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia.
authors.forename: Mohd
authors.initials: M
authors.lastname: Zamri-Saad
authors.affiliation: Department of Veterinary Clinical Studies, Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia.
authors.forename: Abd Wahid
authors.initials: AW
authors.lastname: Haron
authors.affiliation: Department of Veterinary Clinical Studies, Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia.
authors.forename: Abdul Aziz
authors.initials: AA
authors.lastname: Saharee
authors.affiliation: Department of Veterinary Pathology and Microbiology, Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia.
authors.forename: Mohd Azmi Mohd
authors.initials: MAM
authors.lastname: Lila
authors.affiliation: Department of Veterinary Clinical Studies, Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia.
authors.forename: Mohd Jefri
authors.initials: MJ
authors.lastname: Norsidin
mesh_terms: D000818:Animals; D000942:Antigens, Bacterial / Q000276:immunology*; D001344:Autopsy; D001425:Bacterial Outer Membrane Proteins / Q000276:immunology*; D001428:Bacterial Vaccines / Q000276:immunology; D001831:Body Temperature; D002417:Cattle; D002418:Cattle Diseases / Q000097:blood / Q000175:diagnosis* / Q000276:immunology* / Q000382:microbiology; D006403:Hematologic Tests; D007114:Immunization; D010326:Pasteurella Infections / Q000662:veterinary*; D016979:Pasteurella multocida / Q000276:immunology*; D010641:Phenotype
chemical_list: D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins; D001428:Bacterial Vaccines
keywords: Buffalo heifers; Clinico-pathology; Haemato-biochemistry responses; Oral inoculation; Outer membrane protein; Pasteurella multocida type B:2; Subcutaneous inoculation