title: Polymorphisms in MIR499A and MIR125A gene are associated with autoimmune thyroid diseases.
abstract: Single nucleotide polymorphisms (SNPs) of the miR-146a, miR-499a and miR-125a have been shown to be associated with the susceptibility to several autoimmune diseases. This study was conducted to identify the association of SNPs rs2910164, rs57095329, rs3746444 and rs12976445 with autoimmune thyroid diseases (AITDs) in a Chinese Han population.
  
  
  
  We enrolled 1061 patients with AITDs, including 701 patients with Graves' disease (GD) and 360 patients with Hashimoto's thyroiditis (HT), and 938 healthy individuals for a case-control genetic association study. Four SNPs were selected for genotyping by multiplex polymerase chain reaction and ligase detection reaction.
  
  
  
  The frequencies of rs3746444 genotypes in patients with AITD and GD differed significantly from those in the controls. The frequencies of rs12976445 genotypes in patients with HT differed significantly from those in the controls. The frequencies of allele C in HT groups were significantly higher than those in control group. For the rs3746444 polymorphism, genetic associations between the combinational genotype and AITD/GD risk were observed in the dominant model, recessive model, and overdominant model. For the rs12976445 polymorphism, genetic associations between the combinational genotype and HT risk were also found in the dominant model and overdominant model. Moreover, gene-sex interactions were identified by GMDR and 2 × 2 crossover analysis.
  
  
  
  Our results suggest rs3746444 (miR-499a) and rs12976445 (miR-125a) associated with AITD susceptibility and potential gene-sex interactions between the four polymorphisms and AITD.
authors.affiliation: Department of Endocrinology, The First People's Hospital of Xianyang, No. 10 Biyuan West Road, Xianyang 712000, Shaanxi Province, People's Republic of China; Department of Endocrinology, Jinshan Hospital of Fudan University, No. 1508 Longhang Road, Shanghai 201508, People's Republic of China.
authors.forename: TianTian
authors.initials: T
authors.lastname: Cai
authors.affiliation: Department of Nephrology, Xi'an Central Hospital, No.161 Xiwu Road, Xi'an 710003, Shaanxi Province, People's Republic of China.
authors.forename: Jie
authors.initials: J
authors.lastname: Li
authors.affiliation: Department of Endocrinology, Jinshan Hospital of Fudan University, No. 1508 Longhang Road, Shanghai 201508, People's Republic of China.
authors.forename: Xiaofei
authors.initials: X
authors.lastname: An
authors.affiliation: Department of Endocrinology, Jinshan Hospital of Fudan University, No. 1508 Longhang Road, Shanghai 201508, People's Republic of China.
authors.forename: Ni
authors.initials: N
authors.lastname: Yan
authors.affiliation: Department of Endocrinology, Jinshan Hospital of Fudan University, No. 1508 Longhang Road, Shanghai 201508, People's Republic of China.
authors.forename: Danfeng
authors.initials: D
authors.lastname: Li
authors.affiliation: Department of Endocrinology, Jinshan Hospital of Fudan University, No. 1508 Longhang Road, Shanghai 201508, People's Republic of China.
authors.forename: Yanfei
authors.initials: Y
authors.lastname: Jiang
authors.affiliation: Department of Endocrinology, Jinshan Hospital of Fudan University, No. 1508 Longhang Road, Shanghai 201508, People's Republic of China.
authors.forename: Wen
authors.initials: W
authors.lastname: Wang
authors.affiliation: Department of Endocrinology, Jinshan Hospital of Fudan University, No. 1508 Longhang Road, Shanghai 201508, People's Republic of China.
authors.forename: Liangfeng
authors.initials: L
authors.lastname: Shi
authors.affiliation: Department of Endocrinology, Jinshan Hospital of Fudan University, No. 1508 Longhang Road, Shanghai 201508, People's Republic of China.
authors.forename: Qiu
authors.initials: Q
authors.lastname: Qin
authors.affiliation: Department of Endocrinology, Jinshan Hospital of Fudan University, No. 1508 Longhang Road, Shanghai 201508, People's Republic of China.
authors.forename: Ronghua
authors.initials: R
authors.lastname: Song
authors.affiliation: Department of Neurosurgery, The First People's Hospital of Xianyang, No. 10 Biyuan West Road, Xianyang 712000, Shaanxi Province, People's Republic of China.
authors.forename: Guofei
authors.initials: G
authors.lastname: Wang
authors.affiliation: Department of Endocrinology, Jinshan Hospital of Fudan University, No. 1508 Longhang Road, Shanghai 201508, People's Republic of China. Electronic address: hidyfan81@yahoo.com.cn.
authors.forename: Wenjuan
authors.initials: W
authors.lastname: Jiang
authors.affiliation: Department of Endocrinology, Jinshan Hospital of Fudan University, No. 1508 Longhang Road, Shanghai 201508, People's Republic of China. Electronic address: zhangjinan@hotmail.com.
authors.forename: Jin-An
authors.initials: JA
authors.lastname: Zhang
mesh_terms: D000328:Adult; D000483:Alleles; D001327:Autoimmune Diseases / Q000235:genetics*; D016022:Case-Control Studies; D003710:Demography; D004843:Epistasis, Genetic; D005260:Female; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease; D006111:Graves Disease / Q000235:genetics; D050031:Hashimoto Disease / Q000235:genetics*; D006801:Humans; D016015:Logistic Models; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D058011:Multifactor Dimensionality Reduction; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D012307:Risk Factors
chemical_list: C531888:MIRN125 microRNA, human; C548683:MIRN499 microRNA, human; D035683:MicroRNAs
keywords: Autoimmune thyroid diseases (AITD); Graves' disease (GD); Hashimoto's thyroiditis (HT); MicroRNAs; Single nucleotide polymorphism (SNP)