title: Identification of a rhodanese-like protein involved in thiouridine biosynthesis in Thermus thermophilus tRNA.
abstract: Incorporation of a sulfur atom into 2-thioribothymidine (s2T or 5-methyl-2-thiouridine) at position 54 in thermophile tRNA is accomplished by an elaborate system composed of many proteins which confers thermostability to the translation system. We identified ttuD (tRNA-two-thiouridine D) as a gene for the synthesis of s2T54 in Thermus thermophilus. The rhodanese-like protein TtuD enhances the activity of cysteine desulfurases and receives the persulfide generated by cysteine desulfurases in vitro. TtuD also enhances the formation of thiocarboxylated TtuB, the sulfur donor for the tRNA sulfurtransferase TtuA. Since cysteine desulfurases are the first enzymes in the synthesis of s2T and other sulfur-containing compounds, TtuD has a role to direct sulfur flow to s2T synthesis.
authors.affiliation: Biotechnology Research Institute for Drug Discovery, National Institute of Advanced Industrial Science and Technology (AIST), Tokyo, Japan.
authors.forename: Naoki
authors.initials: N
authors.lastname: Shigi
authors.affiliation: Japan Biological Information Research Center (JBIRC), Japan Biological Informatics Consortium (JBIC), Tokyo, Japan.
authors.forename: Shin-Ichi
authors.initials: SI
authors.lastname: Asai
authors.affiliation: Biomedicinal Information Research Center (BIRC), National Institute of Advanced Industrial Science and Technology (AIST), Tokyo, Japan.
authors.forename: Kimitsuna
authors.initials: K
authors.lastname: Watanabe
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D018716:Molecular Mimicry; D012329:RNA, Bacterial / Q000235:genetics / Q000378:metabolism; D012343:RNA, Transfer / Q000235:genetics / Q000378:metabolism*; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D013466:Sulfurtransferases / Q000235:genetics / Q000378:metabolism*; D016963:Thermus thermophilus / Q000737:chemistry* / Q000201:enzymology; D013884:Thiosulfate Sulfurtransferase / Q000235:genetics / Q000378:metabolism; D013891:Thiouridine / Q000031:analogs & derivatives* / Q000378:metabolism
chemical_list: C105273:2-thiouridine; D001426:Bacterial Proteins; D012329:RNA, Bacterial; D011994:Recombinant Proteins; D013891:Thiouridine; C022856:2-thioribothymidine; D012343:RNA, Transfer; D013466:Sulfurtransferases; C024550:thiouridine-tRNA sulfurtransferase; D013884:Thiosulfate Sulfurtransferase
keywords: rhodanese; sulfur; tRNA