title: Effects of fluoridation of porcine hydroxyapatite on osteoblastic activity of human MG63 cells.
abstract: Biological hydroxyapatite, derived from animal bones, is the most widely used bone substitute in orthopedic and dental treatments. Fluorine is the trace element involved in bone remodeling and has been confirmed to promote osteogenesis when administered at the appropriate dose. To take advantage of this knowledge, fluorinated porcine hydroxyapatite (FPHA) incorporating increasing levels of fluoride was derived from cancellous porcine bone through straightforward chemical and thermal treatments. Physiochemical characteristics, including crystalline phases, functional groups and dissolution behavior, were investigated on this novel FPHA. Human osteoblast-like MG63 cells were cultured on the FPHA to examine cell attachment, cytoskeleton, proliferation and osteoblastic differentiation forin vitrocellular evaluation. Results suggest that fluoride ions released from the FPHA play a significant role in stimulating osteoblastic activityin vitro, and appropriate level of fluoridation (1.5 to 3.1 atomic percents of fluorine) for the FPHA could be selected with high potential for use as a bone substitute.
authors.affiliation: Guanghua School of Stomatology, Hospital of Stomatology, Sun Yat-sen University, 56 LingYuan Road West, Guangzhou 510055, Guangdong, People's Republic of China; Guangdong Provincial Key Laboratory of Stomatology, 74 ZhongShan 2 Road, Guangzhou 510055, Guangdong, People's Republic of China.
authors.forename: Zhipeng
authors.initials: Z
authors.lastname: Li
authors.affiliation: Guanghua School of Stomatology, Hospital of Stomatology, Sun Yat-sen University, 56 LingYuan Road West, Guangzhou 510055, Guangdong, People's Republic of China; Guangdong Provincial Key Laboratory of Stomatology, 74 ZhongShan 2 Road, Guangzhou 510055, Guangdong, People's Republic of China.
authors.forename: Baoxin
authors.initials: B
authors.lastname: Huang
authors.affiliation: Guanghua School of Stomatology, Hospital of Stomatology, Sun Yat-sen University, 56 LingYuan Road West, Guangzhou 510055, Guangdong, People's Republic of China; Guangdong Provincial Key Laboratory of Stomatology, 74 ZhongShan 2 Road, Guangzhou 510055, Guangdong, People's Republic of China.
authors.forename: Sui
authors.initials: S
authors.lastname: Mai
authors.affiliation: Guanghua School of Stomatology, Hospital of Stomatology, Sun Yat-sen University, 56 LingYuan Road West, Guangzhou 510055, Guangdong, People's Republic of China; Guangdong Provincial Key Laboratory of Stomatology, 74 ZhongShan 2 Road, Guangzhou 510055, Guangdong, People's Republic of China.
authors.forename: Xiayi
authors.initials: X
authors.lastname: Wu
authors.affiliation: Guanghua School of Stomatology, Hospital of Stomatology, Sun Yat-sen University, 56 LingYuan Road West, Guangzhou 510055, Guangdong, People's Republic of China; Guangdong Provincial Key Laboratory of Stomatology, 74 ZhongShan 2 Road, Guangzhou 510055, Guangdong, People's Republic of China.
authors.forename: Hanqing
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Guanghua School of Stomatology, Hospital of Stomatology, Sun Yat-sen University, 56 LingYuan Road West, Guangzhou 510055, Guangdong, People's Republic of China; Guangdong Provincial Key Laboratory of Stomatology, 74 ZhongShan 2 Road, Guangzhou 510055, Guangdong, People's Republic of China.
authors.forename: Wei
authors.initials: W
authors.lastname: Qiao
authors.affiliation: Guanghua School of Stomatology, Hospital of Stomatology, Sun Yat-sen University, 56 LingYuan Road West, Guangzhou 510055, Guangdong, People's Republic of China; Guangdong Provincial Key Laboratory of Stomatology, 74 ZhongShan 2 Road, Guangzhou 510055, Guangdong, People's Republic of China.
authors.forename: Xin
authors.initials: X
authors.lastname: Luo
authors.affiliation: Guanghua School of Stomatology, Hospital of Stomatology, Sun Yat-sen University, 56 LingYuan Road West, Guangzhou 510055, Guangdong, People's Republic of China; Guangdong Provincial Key Laboratory of Stomatology, 74 ZhongShan 2 Road, Guangzhou 510055, Guangdong, People's Republic of China.
authors.forename: Zhuofan
authors.initials: Z
authors.lastname: Chen
keywords: biological hydroxyapatite; fluoridation; material-cell interactions; osteoblasts; porcine hydroxyapatite