title: Platelets Inhibit Migration of Canine Osteosarcoma Cells.
abstract: The interaction between platelets and tumour cells is important for tumour growth and metastasis. Thrombocytopenia or antiplatelet treatment negatively impact on cancer metastasis, demonstrating potentially important roles for platelets in tumour progression. To our knowledge, there is no information regarding the role of platelets in cancer progression in dogs. This study was designed to test whether canine platelets affected the migratory behaviour of three canine osteosarcoma cell lines and to give insights of molecular mechanisms. Intact platelets, platelet lysate and platelet releasate inhibited the migration of canine osteosarcoma cell lines. Addition of blood leucocytes to the platelet samples did not alter the inhibitory effect on migration. Platelet treatment also significantly downregulated the transcriptional levels of SNAI2 and TWIST1 genes. The interaction between canine platelets or molecules released during platelet activation and these tumour cell lines inhibits their migration, which suggests that canine platelets might antagonize metastasis of canine osteosarcoma. This effect is probably due to, at least in part, downregulation of genes related to epithelial-mesenchymal transition.
authors.affiliation: Department of Pathobiology and Population Medicine, College of Veterinary Medicine, Mississippi State University, USA. Electronic address: bulla@cvm.msstate.edu.
authors.forename: S C
authors.initials: SC
authors.lastname: Bulla
authors.affiliation: Department of Pathobiology and Population Medicine, College of Veterinary Medicine, Mississippi State University, USA.
authors.forename: P R
authors.initials: PR
authors.lastname: Badial
authors.affiliation: Department of Pathobiology and Population Medicine, College of Veterinary Medicine, Mississippi State University, USA.
authors.forename: R C
authors.initials: RC
authors.lastname: Silva
authors.affiliation: Department of Clinical Sciences and Animal Health Center, College of Veterinary Medicine, Mississippi State University, MS, USA.
authors.forename: K
authors.initials: K
authors.lastname: Lunsford
authors.affiliation: Department of Pathobiology and Population Medicine, College of Veterinary Medicine, Mississippi State University, USA.
authors.forename: C
authors.initials: C
authors.lastname: Bulla
mesh_terms: D000818:Animals; D001792:Blood Platelets / Q000378:metabolism*; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000502:physiology*; D018920:Coculture Techniques; D004195:Disease Models, Animal; D018450:Disease Progression; D004285:Dogs; D058750:Epithelial-Mesenchymal Transition / Q000502:physiology; D012516:Osteosarcoma / Q000473:pathology*; D016133:Polymerase Chain Reaction
keywords: cell migration; dog; osteosarcoma; platelets