title: Quantitative and Selective Analysis of Feline Growth Related Proteins Using Parallel Reaction Monitoring High Resolution Mass Spectrometry.
abstract: Today immunoassays are widely used in veterinary medicine, but lack of species specific assays often necessitates the use of assays developed for human applications. Mass spectrometry (MS) is an attractive alternative due to high specificity and versatility, allowing for species-independent analysis. Targeted MS-based quantification methods are valuable complements to large scale shotgun analysis. A method referred to as parallel reaction monitoring (PRM), implemented on Orbitrap MS, has lately been presented as an excellent alternative to more traditional selected reaction monitoring/multiple reaction monitoring (SRM/MRM) methods. The insulin-like growth factor (IGF)-system is not well described in the cat but there are indications of important differences between cats and humans. In feline medicine IGF-I is mainly analyzed for diagnosis of growth hormone disorders but also for research, while the other proteins in the IGF-system are not routinely analyzed within clinical practice. Here, a PRM method for quantification of IGF-I, IGF-II, IGF binding protein (BP) -3 and IGFBP-5 in feline serum is presented. Selective quantification was supported by the use of a newly launched internal standard named QPrEST™. Homology searches demonstrated the possibility to use this standard of human origin for quantification of the targeted feline proteins. Excellent quantitative sensitivity at the attomol/μL (pM) level and selectivity were obtained. As the presented approach is very generic we show that high resolution mass spectrometry in combination with PRM and QPrEST™ internal standards is a versatile tool for protein quantitation across multispecies.
authors.affiliation: Analytical Chemistry, Department of Chemistry-BMC and Science for Life Laboratory, Uppsala University, Uppsala, Sweden.
authors.forename: Mårten
authors.initials: M
authors.lastname: Sundberg
authors.affiliation: Department of Clinical Sciences, Swedish University of Agricultural Sciences, Uppsala, Sweden.|Clinical Pathology Laboratory, University Animal Hospital, Swedish University of Agricultural Sciences, Uppsala, Sweden.
authors.forename: Emma M
authors.initials: EM
authors.lastname: Strage
authors.affiliation: Analytical Chemistry, Department of Chemistry-BMC and Science for Life Laboratory, Uppsala University, Uppsala, Sweden.
authors.forename: Jonas
authors.initials: J
authors.lastname: Bergquist
authors.affiliation: Department of Clinical Sciences, Swedish University of Agricultural Sciences, Uppsala, Sweden.
authors.forename: Bodil S
authors.initials: BS
authors.lastname: Holst
authors.affiliation: Analytical Chemistry, Department of Chemistry-BMC and Science for Life Laboratory, Uppsala University, Uppsala, Sweden.
authors.forename: Margareta
authors.identifier: http://orcid.org/0000-0003-1471-7291
authors.initials: M
authors.lastname: Ramström
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D057205:Automation, Laboratory; D002138:Calibration; D002415:Cats; D003920:Diabetes Mellitus / Q000097:blood / Q000175:diagnosis / Q000662:veterinary*; D006801:Humans; D018972:Insulin-Like Growth Factor Binding Protein 3 / Q000097:blood*; D018975:Insulin-Like Growth Factor Binding Protein 5 / Q000097:blood*; D007334:Insulin-Like Growth Factor I / Q000378:metabolism*; D007335:Insulin-Like Growth Factor II / Q000378:metabolism*; D013058:Mass Spectrometry / Q000295:instrumentation / Q000379:methods / Q000662:veterinary*; D010446:Peptide Fragments / Q000032:analysis; D012015:Reference Standards
chemical_list: D018972:Insulin-Like Growth Factor Binding Protein 3; D018975:Insulin-Like Growth Factor Binding Protein 5; D010446:Peptide Fragments; D007334:Insulin-Like Growth Factor I; D007335:Insulin-Like Growth Factor II