title: Immunomodulatory effect of characterized extract of Zataria multiflora on Th1, Th2and Th17in normal and Th2polarization state.
abstract: The effect of the extract of Zataria multiflora (Z. multiflora) on IFN-γ, FOXP3, IL-4, TGF-β, and IL-17 gene expression was evaluated in cultured splenocytes obtained from control, nontreated asthma or sensitized mice (group S), Sensetized animals treated with dexamethasone or three concentrations of Z. multiflora extract (200, 400 and 800 μg/ml) (n = 6, for each group). IFN-γ and FOXP3 gene expressions were significantly decreased (P < 0.001 for both cases) but IL-4 (P < 0.001) and IL-17 (P < 0.05) were increased in group S compared to control group. Z. Multiflora extract 800 μg/ml, significantly upregulated IFN-γ gene expression (P < 0.01) and its 400 and 800 μg/ml concentrations increased FOXP3 gene expression (P < 0.05 and P < 0.001, respectively) compared to group S. Z. multiflora extract at all concentrations (200, 400 and 800 μg/ml) decreased TGF-β gene expression and its lowest concentration significantly reduced IL-17 gene expression compared to group S (P < 0.001 for all cases). Only IL-4 and TGF-β gene expression was significantly decreased following treatment with dexamethasone (P < 0.001 for both cases). The results indicated an increase in IFN-γ and FOXP3 but decrease in TGF-β and IL-17 gene expression profile in sensitized splenocytes treated with the extract, which might be partially due to the presence of one of its constituent, carvacrol.
authors.affiliation: Department of Physiology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran; Neurogenic Inflammation Research Centre, Mashhad University of Medical Sciences, Mashhad, Iran.
authors.forename: Majid
authors.initials: M
authors.lastname: Kianmehr
authors.affiliation: Neurogenic Inflammation Research Centre, Mashhad University of Medical Sciences, Mashhad, Iran.
authors.forename: Abdolrahim
authors.initials: A
authors.lastname: Rezaei
authors.affiliation: Department of Physiology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran; Neurocognitive Research Centre, Mashhad University of Medical Sciences, Mashhad, Iran.
authors.forename: Mahmoud
authors.initials: M
authors.lastname: Hosseini
authors.affiliation: Department of Physiology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
authors.forename: Mohammad Reza
authors.initials: MR
authors.lastname: Khazdair
authors.affiliation: Department of Physiology and Pharmacology, School of Medicine, North Khorasan University of Medical Sciences, Bojnurd, Iran.
authors.forename: Ramin
authors.initials: R
authors.lastname: Rezaee
authors.affiliation: Department of Pharmacology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
authors.forename: Vahid Reza
authors.initials: VR
authors.lastname: Askari
authors.affiliation: Department of Physiology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran; Neurogenic Inflammation Research Centre, Mashhad University of Medical Sciences, Mashhad, Iran. Electronic address: boskabadymh@mums.ac.ir.
authors.forename: Mohammad Hossein
authors.initials: MH
authors.lastname: Boskabady
mesh_terms: D000818:Animals; D051858:Forkhead Transcription Factors / Q000235:genetics / Q000378:metabolism; D007155:Immunologic Factors / Q000494:pharmacology*; D007371:Interferon-gamma / Q000235:genetics / Q000378:metabolism; D020381:Interleukin-17 / Q000235:genetics / Q000378:metabolism; D015847:Interleukin-4 / Q000235:genetics / Q000378:metabolism; D019686:Lamiaceae / Q000737:chemistry*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D010936:Plant Extracts / Q000494:pharmacology*; D012333:RNA, Messenger / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D012130:Respiratory Hypersensitivity; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013154:Spleen / Q000187:drug effects / Q000276:immunology* / Q000378:metabolism; D018417:Th1 Cells / Q000187:drug effects / Q000276:immunology* / Q000378:metabolism; D058504:Th17 Cells / Q000187:drug effects / Q000276:immunology* / Q000378:metabolism; D018418:Th2 Cells / Q000187:drug effects / Q000276:immunology* / Q000378:metabolism; D016212:Transforming Growth Factor beta / Q000235:genetics / Q000378:metabolism
chemical_list: D051858:Forkhead Transcription Factors; C498833:Foxp3 protein, mouse; C000611649:IFNG protein, mouse; D007155:Immunologic Factors; D020381:Interleukin-17; D010936:Plant Extracts; D012333:RNA, Messenger; D016212:Transforming Growth Factor beta; D015847:Interleukin-4; D007371:Interferon-gamma
keywords: Cytokines; Gene expression; Immune system; Real time PCR; Splenocyte; Zataria multiflora