title: High-throughput downstream process development for cell-based products using aqueous two-phase systems (ATPS) - A case study.
abstract: The availability of preparative-scale downstream processing strategies for cell-based products presents a critical juncture between fundamental research and clinical development. Aqueous two-phase systems (ATPS) present a gentle, scalable, label-free, and cost-effective method for cell purification, and are thus a promising tool for downstream processing of cell-based therapeutics. Here, the application of a previously developed robotic screening platform that enables high-throughput cell partitioning analysis in ATPS is reported. In the present case study a purification strategy for two model cell lines based on high-throughput screening (HTS)-data and countercurrent distribution (CCD)-modeling, and validated the CCD-model experimentally is designed. The obtained data are shown an excellent congruence between CCD-model and experimental data, indicating that CCD-models in combination with HTS-data are a powerful tool in downstream process development. Finally, the authors are shown that while cell cycle phase significantly influences cell partitioning, cell type specific differences in surface properties are the main driving force in charge-dependent separation of HL-60 and L929 cells. In order to design a highly robust purification process it is, however, advisable to maintain constant growth conditions.
authors.affiliation: Karlsruhe Institute of Technology (KIT), Institute of Process Engineering in Life Science, Section IV: Biomolecular Separation Engineering (MAB), Karlsruhe, Germany.
authors.forename: Sarah
authors.initials: S
authors.lastname: Zimmermann
authors.affiliation: Karlsruhe Institute of Technology (KIT), Institute of Process Engineering in Life Science, Section IV: Biomolecular Separation Engineering (MAB), Karlsruhe, Germany.
authors.forename: Christian
authors.initials: C
authors.lastname: Scheeder
authors.affiliation: Karlsruhe Institute of Technology (KIT), Institute of Process Engineering in Life Science, Section IV: Biomolecular Separation Engineering (MAB), Karlsruhe, Germany.
authors.forename: Philipp K
authors.initials: PK
authors.lastname: Zimmermann
authors.affiliation: Novo Nordisk A/S, Biopharm Purification Development & Virology, Gentofte, Denmark.
authors.forename: Are
authors.initials: A
authors.lastname: Bogsnes
authors.affiliation: Novo Nordisk A/S, Diabetes Research, Måløv, Denmark.
authors.forename: Mattias
authors.initials: M
authors.lastname: Hansson
authors.affiliation: Novo Nordisk A/S, CMC Project Planning & Management, Bagsvaerd, Denmark.
authors.forename: Arne
authors.initials: A
authors.lastname: Staby
authors.affiliation: Karlsruhe Institute of Technology (KIT), Institute of Process Engineering in Life Science, Section IV: Biomolecular Separation Engineering (MAB), Karlsruhe, Germany.
authors.forename: Jürgen
authors.initials: J
authors.lastname: Hubbuch
mesh_terms: D001709:Biotechnology / Q000379:methods*; D002453:Cell Cycle / Q000502:physiology; D011092:Polyethylene Glycols / Q000737:chemistry; D014867:Water / Q000737:chemistry
chemical_list: D014867:Water; D011092:Polyethylene Glycols
keywords: Aqueous two-phase systems; Cell cycle analysis; Countercurrent distribution; High-throughput screening; Label-free cell separation