title: Methods to Study Antimicrobial Resistance in Campylobacter jejuni.
abstract: Campylobacter jejuni is a leading bacterial cause of foodborne gastroenteritis worldwide and is increasingly resistant to clinically important antibiotics. Detection of antibiotic resistance in C. jejuni can be performed with both phenotypic and genotypic methods. In this chapter, we describe the most commonly used molecular biology methods for detection of resistance to clinically important antibiotics. These methods can be employed in both clinical and research settings to facilitate clinical therapy and to monitor the emergence and dissemination of antibiotic-resistant C. jejuni.
authors.affiliation: Department of Veterinary Microbiology and Preventive Medicine, Iowa State University, 1116 Veterinary Medicine Complex, 1600 S. 16th Street, Ames, IA, 50011, USA.|Department of Veterinary Diagnostic and Production Animal Medicine, Iowa State University, 1116 Veterinary Medicine Complex, 1600 S. 16th Street, Ames, IA, 50011, USA.
authors.forename: Orhan
authors.initials: O
authors.lastname: Sahin
authors.affiliation: Department of Veterinary Microbiology and Preventive Medicine, Iowa State University, 1116 Veterinary Medicine Complex, 1600 S. 16th Street, Ames, IA, 50011, USA.
authors.forename: Zhangqi
authors.initials: Z
authors.lastname: Shen
authors.affiliation: Department of Veterinary Microbiology and Preventive Medicine, Iowa State University, 1116 Veterinary Medicine Complex, 1600 S. 16th Street, Ames, IA, 50011, USA. zhang123@iastate.edu.
authors.forename: Qijing
authors.initials: Q
authors.lastname: Zhang
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D002169:Campylobacter Infections / Q000382:microbiology; D016123:Campylobacter jejuni / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development; D002939:Ciprofloxacin / Q000494:pharmacology; D003470:Culture Media; D017931:DNA Primers / Q000737:chemistry / Q000378:metabolism; D004269:DNA, Bacterial / Q000235:genetics*; D024901:Drug Resistance, Multiple, Bacterial / Q000235:genetics*; D005759:Gastroenteritis / Q000382:microbiology; D055786:Gene Knockout Techniques; D005818:Genetic Engineering / Q000379:methods*; D006801:Humans; D008826:Microbial Sensitivity Tests; D016133:Polymerase Chain Reaction / Q000379:methods*; D017422:Sequence Analysis, DNA
chemical_list: D000900:Anti-Bacterial Agents; D003470:Culture Media; D017931:DNA Primers; D004269:DNA, Bacterial; D002939:Ciprofloxacin
keywords: Antimicrobial resistance; Campylobacter jejuni; Molecular detection; PCR; Sequencing