title: Changes of ammonia, urea contents and transaminase activity in the body during aerial exposure and ammonia loading in Chinese loach Paramisgurnus dabryanus.
abstract: The Paramisgurnus dabryanus was exposed to 30 mmol L-1NH4Cl solution and air to assessing the change of body ammonia and urea contents and the activities of alanine aminotransferase (ALT) and aspartate transaminase (AST). After 48 h of ammonia exposure, ammonia concentration in the plasma, brain, liver and muscle were 3.3-fold, 5.6-fold, 3.5-fold and 4.2-fold, respectively, those of the control values. Plasma, brain, liver and muscle ammonia concentrations increased to 2.2-fold, 3.3-fold, 2.5-fold and 2.9-fold, respectively, those of control values in response to 48 h of aerial exposure. Within the given treatment (ammonia or aerial exposure), there was no change in plasma, brain and liver urea concentrations between exposure durations. The plasma ALT activity was significantly affected by exposure time during aerial exposure, while the liver ALT activity was not affected by ammonia or aerial exposure. Exposure to NH4Cl or air had no effect on either plasma or liver AST activity. Our results suggested that P. dabryanus could accumulate quite high level of internal ammonia because of the high ammonia tolerance in its cells and tissues, and NH3volatilization would be a possible ammonia detoxification strategy in P. dabryanus. Urea synthesis was not an effective mechanism to deal with environmental or internal ammonia problem. The significant increase of ALT activity in plasma during aerial exposure, indicating that alanine synthesis through certain amino acid catabolism may be subsistent in P. dabryanus.
authors.affiliation: College of Animal Science and Technology, Anhui Agricultural University, Hefei, 230036, China.
authors.forename: Yun-Long
authors.initials: YL
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Freshwater Animal Breeding, Ministry of Agriculture, College of Fisheries, Huazhong Agricultural University, Wuhan, 430070, China.
authors.forename: Hai-Long
authors.initials: HL
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Freshwater Animal Breeding, Ministry of Agriculture, College of Fisheries, Huazhong Agricultural University, Wuhan, 430070, China.
authors.forename: Ling-Yu
authors.initials: LY
authors.lastname: Wang
authors.affiliation: Key Laboratory of Freshwater Animal Breeding, Ministry of Agriculture, College of Fisheries, Huazhong Agricultural University, Wuhan, 430070, China.
authors.forename: Bei-Yi
authors.initials: BY
authors.lastname: Gu
authors.affiliation: Key Laboratory of Freshwater Animal Breeding, Ministry of Agriculture, College of Fisheries, Huazhong Agricultural University, Wuhan, 430070, China. fanqixue@mail.hzau.edu.cn.
authors.forename: Qi-Xue
authors.initials: QX
authors.lastname: Fan
mesh_terms: D000388:Air; D000410:Alanine Transaminase / Q000097:blood / Q000378:metabolism*; D000641:Ammonia / Q000097:blood / Q000493:pharmacokinetics*; D000818:Animals; D001219:Aspartate Aminotransferases / Q000097:blood / Q000378:metabolism*; D001921:Brain / Q000378:metabolism; D003531:Cypriniformes / Q000097:blood / Q000378:metabolism*; D029941:Fish Proteins / Q000097:blood / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D009132:Muscles / Q000378:metabolism; D014508:Urea / Q000097:blood / Q000378:metabolism*
chemical_list: D029941:Fish Proteins; D000641:Ammonia; D014508:Urea; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase
keywords: Aerial exposure; Ammonia; Ammonia loading; Paramisgurnus dabryanus; Urea