title: Expression of Fibroblast Activating Protein and Correlation with Histological Grade, Mitotic Index and Ki67 Expression in Canine Mast Cell Tumours.
abstract: Fibroblast activating protein (FAP) is a membrane serine protease expressed by activated fibroblasts, particularly tumour associated fibroblasts (TAFs). FAP expression has not been reported in canine mast cell tumours (MCTs). The objective of this study was to evaluate the expression of FAP in TAFs and its correlation with histological grade, mitotic index and Ki67 expression in canine MCTs. FAP expression was evaluated by immunohistochemistry (IHC) in 30 canine MCTs. Twenty-eight (90%) of the MCTs expressed FAP in the stroma, 16 cases showed low to intermediate FAP score and 14 cases had a high FAP score. FAP was correlated positively with both Patnaik (P = 0.007) and Kiupel (P = 0.008) grading systems, mitotic index (P = 0.0008) and Ki67 expression (P = 0.009). High stromal FAP expression could be a potential negative prognostic factor in canine MCTs.
authors.affiliation: University of Cambridge, Department of Veterinary Medicine, Madingley Road, Cambridge, UK. Electronic address: ag847@cam.ac.uk.
authors.forename: A
authors.initials: A
authors.lastname: Giuliano
authors.affiliation: Universidade Federal de Minas Gerais, Department of Veterinary Medicine and Surgery, Brazil.
authors.forename: R
authors.initials: R
authors.lastname: Dos Santos Horta
authors.affiliation: University of Cambridge, Department of Veterinary Medicine, Madingley Road, Cambridge, UK.
authors.forename: F
authors.initials: F
authors.lastname: Constantino-Casas
authors.affiliation: University of Cambridge, Department of Veterinary Medicine, Madingley Road, Cambridge, UK.
authors.forename: T
authors.initials: T
authors.lastname: Hoather
authors.affiliation: University of Cambridge, Department of Veterinary Medicine, Madingley Road, Cambridge, UK.
authors.forename: J
authors.initials: J
authors.lastname: Dobson
mesh_terms: D000818:Animals; D004283:Dog Diseases / Q000378:metabolism / Q000473:pathology*; D004285:Dogs; D010450:Endopeptidases; D005347:Fibroblasts / Q000473:pathology*; D018093:Gelatinases / Q000096:biosynthesis*; D007150:Immunohistochemistry; D019394:Ki-67 Antigen / Q000096:biosynthesis; D012515:Mast-Cell Sarcoma / Q000662:veterinary*; D034801:Mastocytoma / Q000662:veterinary*; D008565:Membrane Proteins / Q000096:biosynthesis*; D008940:Mitotic Index; D060787:Neoplasm Grading; D012697:Serine Endopeptidases / Q000096:biosynthesis*; D000099167:Fibroblast Activation Protein Alpha
chemical_list: D010450:Endopeptidases; D018093:Gelatinases; D019394:Ki-67 Antigen; D008565:Membrane Proteins; D012697:Serine Endopeptidases; D000099167:Fibroblast Activation Protein Alpha
keywords: dog; fibroblast activating protein; mast cell tumour