title: Roles of mitochondrial transcription factor A and microRNA‑590‑3p in the development of colon cancer.
abstract: Mitochondrial transcription factor A (TFAM) participates in the process of mitochondrial DNA replication and transcription. microRNAs (miRNAs) serve an important role in the regulation of gene expression. However, the roles of TFAM and certain miRNAs and their associations in the development of numerous cancer types remain unclear. The current study demonstrated that the expression of TFAM was significantly upregulated in colon cancer compared with the normal tissue, while the expression of miRNA‑590‑3p (miR‑590‑3p) was predicted with a high score using miRWalk software, and the luciferase assay demonstrated that TFAM was the direct target of miRNA‑590‑3p. miR‑590‑3p exhibited high expression levels in both colon cancer tissue and the SW480 cell line. Furthermore, downregulated expression of miR‑590‑3p significantly inhibited the growth of SW480 cells, which was consistent with results indicating downregulated expression of TFAM in SW480 cells from a previous study. In summary, the results of the current study concluded that miR‑590‑3p, via direct targeting of TFAM, may serve an important role in the tumorigenesis of colon cancer, and may be a promising target for colon cancer therapeutics.
authors.affiliation: Department of Gastrointestinal Surgery, First Affiliated Hospital of Sun Yat‑Sen Univesity, Guangzhou, Guangdong 510080, P.R. China.
authors.forename: Kaiming
authors.initials: K
authors.lastname: Wu
authors.affiliation: Department of Pancreato‑Biliary Surgery, First Affiliated Hospital of Sun Yat‑Sen Univesity, Guangzhou, Guangdong 510080, P.R. China.
authors.forename: Zhenxian
authors.initials: Z
authors.lastname: Zhao
authors.affiliation: Department of Gastroenterology and Hepatology, First Affiliated Hospital of Sun Yat‑Sen Univesity, Guangzhou, Guangdong 510080, P.R. China.
authors.forename: Yinglian
authors.initials: Y
authors.lastname: Xiao
authors.affiliation: Department of Gastrointestinal Surgery, First Affiliated Hospital of Sun Yat‑Sen Univesity, Guangzhou, Guangdong 510080, P.R. China.
authors.forename: Jianjun
authors.initials: J
authors.lastname: Peng
authors.affiliation: Department of Gastrointestinal Surgery, First Affiliated Hospital of Sun Yat‑Sen Univesity, Guangzhou, Guangdong 510080, P.R. China.
authors.forename: Jianhui
authors.initials: J
authors.lastname: Chen
authors.affiliation: Department of Gastrointestinal Surgery, First Affiliated Hospital of Sun Yat‑Sen Univesity, Guangzhou, Guangdong 510080, P.R. China.
authors.forename: Yulong
authors.initials: Y
authors.lastname: He
mesh_terms: D045744:Cell Line, Tumor; D049109:Cell Proliferation; D002478:Cells, Cultured; D003110:Colonic Neoplasms / Q000235:genetics* / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D015870:Gene Expression; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease; D006801:Humans; D035683:MicroRNAs / Q000235:genetics*; D024101:Mitochondrial Proteins / Q000235:genetics* / Q000378:metabolism; D034622:RNA Interference; D012333:RNA, Messenger / Q000235:genetics; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism
chemical_list: D004268:DNA-Binding Proteins; C568221:MIRN590 microRNA, human; D035683:MicroRNAs; D024101:Mitochondrial Proteins; D012333:RNA, Messenger; D014157:Transcription Factors; C422562:mitochondrial transcription factor A