title: Neuronal Ndrg4 Is Essential for Nodes of Ranvier Organization in Zebrafish.
abstract: Axon ensheathment by specialized glial cells is an important process for fast propagation of action potentials. The rapid electrical conduction along myelinated axons is mainly due to its saltatory nature characterized by the accumulation of ion channels at the nodes of Ranvier. However, how these ion channels are transported and anchored along axons is not fully understood. We have identified N-myc downstream-regulated gene 4, ndrg4, as a novel factor that regulates sodium channel clustering in zebrafish. Analysis of chimeric larvae indicates that ndrg4 functions autonomously within neurons for sodium channel clustering at the nodes. Molecular analysis of ndrg4 mutants shows that expression of snap25 and nsf are sharply decreased, revealing a role of ndrg4 in controlling vesicle exocytosis. This uncovers a previously unknown function of ndrg4 in regulating vesicle docking and nodes of Ranvier organization, at least through its ability to finely tune the expression of the t-SNARE/NSF machinery.
authors.affiliation: U1195, Inserm, University Paris Sud, University Paris-Saclay, Kremlin-Bicêtre, France.
authors.forename: Laura
authors.initials: L
authors.lastname: Fontenas
authors.affiliation: Institut Curie, PSL Research University, Paris, France.
authors.forename: Flavia
authors.initials: F
authors.lastname: De Santis
authors.affiliation: Institut Curie, PSL Research University, Paris, France.
authors.forename: Vincenzo
authors.initials: V
authors.lastname: Di Donato
authors.affiliation: U1195, Inserm, University Paris Sud, University Paris-Saclay, Kremlin-Bicêtre, France.
authors.forename: Cindy
authors.initials: C
authors.lastname: Degerny
authors.affiliation: U1195, Inserm, University Paris Sud, University Paris-Saclay, Kremlin-Bicêtre, France.
authors.forename: Béatrice
authors.identifier: http://orcid.org/0000-0001-9338-2885
authors.initials: B
authors.lastname: Chambraud
authors.affiliation: Institut Curie, PSL Research University, Paris, France.
authors.forename: Filippo
authors.identifier: http://orcid.org/0000-0001-8551-2846
authors.initials: F
authors.lastname: Del Bene
authors.affiliation: U1195, Inserm, University Paris Sud, University Paris-Saclay, Kremlin-Bicêtre, France.
authors.forename: Marcel
authors.initials: M
authors.lastname: Tawk
mesh_terms: D000818:Animals; D001369:Axons / Q000378:metabolism; D005089:Exocytosis / Q000235:genetics; D005786:Gene Expression Regulation; D006801:Humans; D009124:Muscle Proteins / Q000235:genetics* / Q000378:metabolism; D052066:N-Ethylmaleimide-Sensitive Proteins / Q000096:biosynthesis* / Q000235:genetics; D009457:Neuroglia / Q000378:metabolism; D009474:Neurons / Q000378:metabolism; D011901:Ranvier's Nodes / Q000235:genetics* / Q000378:metabolism; D012583:Schwann Cells; D015222:Sodium Channels / Q000235:genetics / Q000378:metabolism; D009435:Synaptic Transmission / Q000235:genetics; D050825:Synaptosomal-Associated Protein 25 / Q000096:biosynthesis* / Q000235:genetics; D015027:Zebrafish / Q000235:genetics* / Q000378:metabolism; D029961:Zebrafish Proteins / Q000096:biosynthesis* / Q000235:genetics* / Q000378:metabolism
chemical_list: D009124:Muscle Proteins; C530194:Ndrg4 protein, zebrafish; D015222:Sodium Channels; D050825:Synaptosomal-Associated Protein 25; D029961:Zebrafish Proteins; D052066:N-Ethylmaleimide-Sensitive Proteins; C511348:N-ethylmaleimide sensitive factor, zebrafish