title: Common Lung Microbiome Identified among Mechanically Ventilated Surgical Patients.
abstract: The examination of the pulmonary microbiome in patients with non-chronic disease states has not been extensively examined. Traditional culture based screening methods are often unable to identify bacteria from bronchoalveolar lavage samples. The advancement of next-generation sequencing technologies allows for a culture-independent molecular based analysis to determine the microbial composition in the lung of this patient population. For this study, the Ion Torrent PGM system was used to assess the microbial complexity of culture negative bronchoalveolar lavage samples. A group of samples were identified that all displayed high diversity and similar relative abundance of bacteria. This group consisted of Hydrogenophaga, unclassified Bacteroidetes, Pedobacter, Thauera, and Acinetobacter. These bacteria may be representative of a common non-pathogenic pulmonary microbiome associated within this population of patients.
authors.affiliation: Department of Cell Biology and Immunology, University of North Texas Health Science Center, Fort Worth, Texas, United States of America.|Center for Biosafety and Biosecurity, University of North Texas Health Science Center, Fort Worth, Texas, United States of America.
authors.forename: Ashley D
authors.initials: AD
authors.lastname: Smith
authors.affiliation: Center for Biosafety and Biosecurity, University of North Texas Health Science Center, Fort Worth, Texas, United States of America.|Department of Molecular and Medical Genetics, University of North Texas Health Science Center, Fort Worth, Texas, United States of America.
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Molecular and Medical Genetics, University of North Texas Health Science Center, Fort Worth, Texas, United States of America.
authors.forename: Robert C
authors.initials: RC
authors.lastname: Barber
authors.affiliation: Department of Surgery, University of Texas Southwestern Medical Center, Dallas, Texas, United States of America.
authors.forename: Christian T
authors.initials: CT
authors.lastname: Minshall
authors.affiliation: Department of Surgery, University of Texas Southwestern Medical Center, Dallas, Texas, United States of America.
authors.forename: Ryan M
authors.initials: RM
authors.lastname: Huebinger
authors.affiliation: Center for Biosafety and Biosecurity, University of North Texas Health Science Center, Fort Worth, Texas, United States of America.|Department of Molecular and Medical Genetics, University of North Texas Health Science Center, Fort Worth, Texas, United States of America.
authors.forename: Michael S
authors.identifier: http://orcid.org/0000-0003-2293-8078
authors.initials: MS
authors.lastname: Allen
mesh_terms: D000293:Adolescent; D000328:Adult; D044822:Biodiversity; D018893:Bronchoalveolar Lavage; D005260:Female; D006801:Humans; D008168:Lung / Q000382:microbiology*; D008297:Male; D064307:Microbiota / Q000235:genetics*; D008875:Middle Aged; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012121:Respiration, Artificial / Q000009:adverse effects*; D055815:Young Adult
chemical_list: D012336:RNA, Ribosomal, 16S