title: Establishment and characterization of a cell population derived from a dentigerous cyst.
abstract: BACKGROUND
  Dentigerous cyst (DC) occurs in approximately 20% of jaw cysts, being the second major common odontogenic cyst, after radicular cyst. This oral lesion has the ability to destroy maxillary bones and could be the origin of several odontogenic tumors. However, molecules implicated in its pathogenesis as well as those involved in its neoplastic transformation remain unknown. Here, we established a cell population derived from a DC as an in vitro model for the study of this oral lesion.
  
  
  METHODS
  Cell culture was performed from a DC from a 44-year-old male. Cells were cultured at 37°C in DMEM/F12 medium containing 10% fetal bovine serum. Expression of epithelial markers was analyzed by Western blot and immunofluorescence. Ultrastructural characterization was carried out by transmission electron microscopy. Conditioned media were obtained and characterized by zymography and Western blot.
  
  
  RESULTS
  Cells showed spindle-shaped morphology, but they express epithelial markers, such as cytokeratins and the odontogenic ameloblast-associated protein. The ultrastructural analysis showed well-formed desmosomes present in adhering contiguous cells, confirming the epithelial lineage of this cell population. Cells also contain several vesicles adjacent to plasma membrane, suggesting an active secretion. Indeed, the analysis of the conditioned medium revealed the presence of several secreted proteins, among them the matrix metalloproteinase-2.
  
  
  CONCLUSIONS
  Our work provides a useful model to identify the molecular mechanisms involved in the pathogenesis of DC.
authors.affiliation: Departamento de Infectómica y Patogénesis Molecular, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional, Mexico City, Mexico.
authors.forename: Marcos A
authors.initials: MA
authors.lastname: Muñiz-Lino
authors.affiliation: Departamento de Infectómica y Patogénesis Molecular, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional, Mexico City, Mexico.
authors.forename: Mariana
authors.initials: M
authors.lastname: Rodríguez-Vázquez
authors.affiliation: Departamento de Infectómica y Patogénesis Molecular, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional, Mexico City, Mexico.
authors.forename: Bibiana
authors.initials: B
authors.lastname: Chávez-Munguía
authors.affiliation: Sección de Posgrado e Investigación, Escuela Superior de Medicina del Instituto Politécnico Nacional, Mexico City, Mexico.
authors.forename: Josué Z
authors.initials: JZ
authors.lastname: Ortiz-García
authors.affiliation: Departamento de Infectómica y Patogénesis Molecular, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional, Mexico City, Mexico.
authors.forename: Lorena
authors.initials: L
authors.lastname: González-López
authors.affiliation: Departamento de Infectómica y Patogénesis Molecular, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional, Mexico City, Mexico.
authors.forename: Fidel C
authors.initials: FC
authors.lastname: Hernández-Hernández
authors.affiliation: Departamento de Cirugía Maxilofacial, Hospital Juárez de México, Mexico City, Mexico.
authors.forename: Carlos
authors.initials: C
authors.lastname: Licéaga-Escalera
authors.affiliation: Laboratorio de Investigación en Odontología, ALMARAZ/UBIMED, FES Iztacala, UNAM, State of Mexico, Mexico.
authors.forename: Alejandro
authors.initials: A
authors.lastname: García-Muñoz
authors.affiliation: Departamento de Infectómica y Patogénesis Molecular, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional, Mexico City, Mexico.
authors.forename: Mario A
authors.initials: MA
authors.lastname: Rodríguez
mesh_terms: D000328:Adult; D015153:Blotting, Western; D002478:Cells, Cultured; D003803:Dentigerous Cyst / Q000473:pathology*; D005455:Fluorescent Antibody Technique; D006801:Humans; D008297:Male; D008437:Maxilla / Q000166:cytology / Q000473:pathology; D008439:Maxillary Diseases / Q000473:pathology*
keywords: cell culture; dentigerous cyst; epithelial cells; matrix metalloproteinases; secretion