title: [Inhibitory effect of Mig-7 silencing by retrovirus-mediated shRNA on vasculogenic mimicry, invasion and metastasis of human hepatocellular carcinoma cells in vitro].
abstract: OBJECTIVE
  To explore the inhibitory effect of migration-inducing gene 7 (Mig-7) gene silencing induced by retroviral-mediated small hairpin RNA (shRNA) on vasculogenic mimicry (VM), invasion and metastasis of human hepatocellular carcinoma (HCC) cells in vitro.
  
  
  METHODS
  Two target sequences (Mig-7 shRNA-1 and Mig-7 shRNA-2) and one negative control sequence (Mig-7 shRNA-N) were synthesized. The recombinant retroviral vectors carrying Mig-7 shRNA were constructed, and HCC cell line MHCC-97H were transfected with Mig-7 shRNA-1, Mig-7 shRNA-2, Mig-7 shRNA-N, or the empty vector, or treated with 125 µg/mL recombinant human endostatin (ES). Mig-7 expression in the treated cells was detected using semi-quantitative PCR and Western blotting. The inhibitory effect of Mig-7 silencing on VM formation was investigated in a 3-dimensional cell culture system; the changes in cell adhesion, invasion and migration were assessed with intercellular adhesion assay, Transwell invasion assay and Transwell migration assay, respectively.
  
  
  RESULTS
  The expression of Mig-7 at both mRNA and protein levels decreased significantly, VM formation, invasion and metastasis were suppressed, while intercellular adhesion increased significantly in MHCC-97H cells in Mig-7 shRNA-1 and Mig-7 shRNA-2 groups (P<0.05); such changes were not observed in cells transfected with Mig-7 shRNA-N or the empty vector, nor in cells treated with ES.
  
  
  CONCLUSIONS
  Mig-7 silencing by retroviral-mediated shRNA significantly inhibits VM formation, invasion and metastasis and increases the intercellular adhesion of the HCC cells, while ES does not have such inhibitory effects.
authors.affiliation: Department of General Surgery, Affiliated Hospital of Logistics University of Chinese People's Armed Police Forces, Tianjin 300162, China.E-mail: doctor_qb@163.com.
authors.forename: Bo
authors.initials: B
authors.lastname: Qu
authors.forename: Guan-Nan
authors.initials: GN
authors.lastname: Sheng
authors.forename: Fei
authors.initials: F
authors.lastname: Yu
authors.forename: Guan-Nan
authors.initials: GN
authors.lastname: Chen
authors.forename: Qi
authors.initials: Q
authors.lastname: Lv
authors.forename: Zhong-Peng
authors.initials: ZP
authors.lastname: Mao
authors.forename: Long
authors.initials: L
authors.lastname: Guo
authors.forename: Yi
authors.initials: Y
authors.lastname: Lv
mesh_terms: D006528:Carcinoma, Hepatocellular / Q000235:genetics / Q000473:pathology*; D045744:Cell Line, Tumor; D043169:Endostatins; D020868:Gene Silencing; D005822:Genetic Vectors; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics / Q000473:pathology*; D009363:Neoplasm Proteins / Q000235:genetics*; D012333:RNA, Messenger; D034741:RNA, Small Interfering; D012190:Retroviridae; D014162:Transfection
chemical_list: D043169:Endostatins; D009363:Neoplasm Proteins; D012333:RNA, Messenger; D034741:RNA, Small Interfering; C482285:TOX4 protein, human