title: Macrococcus canis sp. nov., a skin bacterium associated with infections in dogs.
abstract: Gram-stain-positive cocci were isolated from miscellaneous sites of the skin of healthy dogs as well as from infection sites in dogs. The closest relative by sequencing of the 16S rRNA gene was Macrococcus caseolyticus with 99.7 % sequence identity, but compared with M. caseolyticus, the novel strains shared only 90.8 to 93.5 % DNA sequence identity with cpn60, dnaJ, rpoB and sodA partial genes, respectively. The novel strains also exhibited differential phenotypic characteristics from M. caseolyticus, and the majority displayed a visible haemolysis on sheep blood agar, while M. caseolyticus did not have any haemolytic activity. They generated different matrix-assisted laser-desorption/ionization time-of-flight (MALDI-TOF) MS spectral profiles compared with the other species of the genus Macrococcus. Furthermore, strain KM 45013Tshared only 53.7 % DNA-DNA relatedness with the type strain of M. caseolyticus, confirming that they do not belong to the same species. The DNA G+C content of strain KM 45013Twas 36.9 mol%. The most abundant fatty acids were C14 : 0, C18 : 3ω6c (6, 9, 12) and C16 : 0 n alcohol. MK-6 was the menaquinone type of KM 45013T. Cell-wall structure analysis revealed that the peptidoglycan type was A3α l-Lys-Gly2-l-Ser. Based on genotypic and chemotaxonomic characteristics, we propose to classify these strains within a novel species of the genus Macrococcus for which the name Macrococcus canis sp. nov. is proposed. The type strain is KM 45013T(=DSM 101690T=CCOS 969T=CCUG 68920T).
authors.affiliation: Institute of Veterinary Bacteriology, Vetsuisse Faculty, University of Bern, Bern.
authors.forename: Stefanie
authors.initials: S
authors.lastname: Gobeli Brawand
authors.affiliation: Institute of Veterinary Bacteriology, Vetsuisse Faculty, University of Bern, Bern.
authors.forename: Kerstin
authors.initials: K
authors.lastname: Cotting
authors.affiliation: Institute of Veterinary Bacteriology, Vetsuisse Faculty, University of Bern, Bern.|Present address: Institute for Food, Nutrition and Health, ETH Zurich, Zurich, Switzerland.
authors.forename: Elena
authors.initials: E
authors.lastname: Gómez-Sanz
authors.affiliation: Institute of Veterinary Bacteriology, Vetsuisse Faculty, University of Bern, Bern.
authors.forename: Alexandra
authors.initials: A
authors.lastname: Collaud
authors.affiliation: Institute of Veterinary Bacteriology, Vetsuisse Faculty, University of Bern, Bern.
authors.forename: Andreas
authors.initials: A
authors.lastname: Thomann
authors.affiliation: Institute of Veterinary Bacteriology, Vetsuisse Faculty, University of Bern, Bern.
authors.forename: Isabelle
authors.initials: I
authors.lastname: Brodard
authors.affiliation: Institute of Veterinary Bacteriology, Vetsuisse Faculty, University of Bern, Bern.
authors.forename: Sabrina
authors.initials: S
authors.lastname: Rodriguez-Campos
authors.affiliation: Institute of Veterinary Bacteriology, Vetsuisse Faculty, University of Bern, Bern.
authors.forename: Christian
authors.initials: C
authors.lastname: Strauss
authors.affiliation: Institute of Veterinary Bacteriology, Vetsuisse Faculty, University of Bern, Bern.
authors.forename: Vincent
authors.initials: V
authors.lastname: Perreten
mesh_terms: D000818:Animals; D015373:Bacterial Typing Techniques; D001482:Base Composition; D002473:Cell Wall / Q000737:chemistry; D004269:DNA, Bacterial / Q000235:genetics; D004283:Dog Diseases / Q000382:microbiology*; D004285:Dogs / Q000382:microbiology*; D005227:Fatty Acids / Q000737:chemistry; D005798:Genes, Bacterial; D009693:Nucleic Acid Hybridization; D010457:Peptidoglycan / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012867:Skin / Q000382:microbiology*; D017192:Skin Diseases, Bacterial / Q000662:veterinary*; D040141:Staphylococcaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010457:Peptidoglycan; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; C042455:menaquinone 6
keywords: Gram-positive; Staphylococcaceae; animals; cocci