title: Generation and Analysis of Humanized Mouse Model of EBV Infection.
abstract: The recent development of severely immunodeficient mouse strains enabled the production of new-generation humanized mice, in which major components of the human immune system are reconstituted. These new-generation humanized mice can be infected with human pathogenic viruses that do not infect regular mice and target cells of the hematoimmune system. Here we describe the method for preparing humanized mice, infecting them with EBV, and for their virological and immunological analyses. The results obtained from our own mouse models are briefly described.
authors.affiliation: Division of Advanced Medicine for Virus Infections, National Research Institute for Child Health and Development, 2-10-1 Okura, Setagaya-ku, Tokyo, 157-8535, Japan.
authors.forename: Ken-Ichi
authors.initials: KI
authors.lastname: Imadome
authors.affiliation: Department of Allergy and Clinical Immunology, National Research Institute for Child Health and Development, 2-10-1 Okura, Setagaya-ku, Tokyo, 157-8535, Japan. fujiwara-s@ncchd.go.jp.|Division of Hematology and Rheumatology, Department of Medicine, Nihon University School of Medicine, 30-1 Oyaguchikamimachi, Itabashi-ku, Tokyo, 173-8610, Japan. fujiwara-s@ncchd.go.jp.
authors.forename: Shigeyoshi
authors.initials: S
authors.lastname: Fujiwara
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D020031:Epstein-Barr Virus Infections / Q000276:immunology* / Q000821:virology*; D018380:Hematopoietic Stem Cell Transplantation; D006412:Hematopoietic Stem Cells; D004854:Herpesvirus 4, Human / Q000276:immunology*; D064593:Heterografts; D015395:Histocompatibility Antigens Class I / Q000737:chemistry / Q000276:immunology; D054884:Host-Pathogen Interactions / Q000276:immunology*; D006801:Humans; D007107:Immune System / Q000166:cytology / Q000276:immunology / Q000378:metabolism; D008214:Lymphocytes / Q000276:immunology / Q000378:metabolism; D051379:Mice; D016688:Mice, Inbred NOD; D018345:Mice, Knockout; D055503:Protein Multimerization; D018183:Transplantation Chimera; D019562:Viral Load
chemical_list: D015395:Histocompatibility Antigens Class I
keywords: ELISPOT assay; Epstein-Barr virus; Humanized mouse; Immune response; Latent infection; Lymphoproliferative disease; MHC class I tetramer; Rheumatoid arthritis; T cell