title: Three-dimensional label-free imaging and quantification of lipid droplets in live hepatocytes.
abstract: Lipid droplets (LDs) are subcellular organelles with important roles in lipid storage and metabolism and involved in various diseases including cancer, obesity, and diabetes. Conventional methods, however, have limited ability to provide quantitative information on individual LDs and have limited capability for three-dimensional (3-D) imaging of LDs in live cells especially for fast acquisition of 3-D dynamics. Here, we present an optical method based on 3-D quantitative phase imaging to measure the 3-D structural distribution and biochemical parameters (concentration and dry mass) of individual LDs in live cells without using exogenous labelling agents. The biochemical change of LDs under oleic acid treatment was quantitatively investigated, and 4-D tracking of the fast dynamics of LDs revealed the intracellular transport of LDs in live cells.
authors.affiliation: Department of Physics, Korea Advanced Institute of Science and Technology, Daejeon 34141, Republic of Korea.
authors.forename: Kyoohyun
authors.initials: K
authors.lastname: Kim
authors.affiliation: Department of Physics, Korea Advanced Institute of Science and Technology, Daejeon 34141, Republic of Korea.
authors.forename: SeoEun
authors.initials: S
authors.lastname: Lee
authors.affiliation: Department of Physics, Korea Advanced Institute of Science and Technology, Daejeon 34141, Republic of Korea.
authors.forename: Jonghee
authors.initials: J
authors.lastname: Yoon
authors.affiliation: Department of Physics, Korea Advanced Institute of Science and Technology, Daejeon 34141, Republic of Korea.
authors.forename: JiHan
authors.initials: J
authors.lastname: Heo
authors.affiliation: Department of Bio and Brain Engineering, Korea Advanced Institute of Science and Technology, Daejeon 34141, Republic of Korea.
authors.forename: Chulhee
authors.initials: C
authors.lastname: Choi
authors.affiliation: Department of Physics, Korea Advanced Institute of Science and Technology, Daejeon 34141, Republic of Korea.|TOMOCUBE, Inc., Daejeon 34051, Republic of Korea.
authors.forename: YongKeun
authors.initials: Y
authors.lastname: Park
mesh_terms: D045744:Cell Line, Tumor; D003593:Cytoplasm / Q000378:metabolism; D022781:Hepatocytes / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D021621:Imaging, Three-Dimensional / Q000379:methods*; D066292:Lipid Droplets / Q000737:chemistry* / Q000378:metabolism; D050356:Lipid Metabolism; D008856:Microscopy, Fluorescence; D019301:Oleic Acid / Q000494:pharmacology; D059008:Time-Lapse Imaging
chemical_list: D019301:Oleic Acid