title: Valorization of exhausted sugar beet cossettes by successive hydrolysis and two fermentations for the production of bio-products.
abstract: Exhausted sugar beet cossettes (ESBC) show an enormous potential as a source of sugars for the production of bio-products. Enzyme hydrolysis with the combined effect of mainly cellulases, xylanases and pectinases, turned out to be very efficient, obtaining almost double the concentration of sugars measured with the sole action of Celluclast® and β-glucosidase, and increasing 5 times the hydrolysis rate. As the sole pretreatment, ESBC soaked in the hydrolysis buffer were autoclaved, avoiding the application of severe conventional biomass pretreatments. Moreover, a promising alternative for the complete utilization of glucose, xylose, arabinose, mannose and maltose contained in ESBC is proposed in this paper. It consists of sequential fermentation of sugars released in the hydrolysis step to produce bioethanol and lactic acid as main bio-products. Compared to separate fermentations, with this strategy glucose and hemicellulose derived sugars were completely consumed and the 44% of pectin derived sugars.
authors.affiliation: Laboratory of Microbiology, Faculty of Marine and Environmental Sciences, University of Cádiz, Pol. Río San Pedro s/n, Puerto Real, Spain. Electronic address: anabelen.diaz@uca.es.
authors.forename: A B
authors.initials: AB
authors.lastname: Díaz
authors.affiliation: Department of Chemical Engineering and Food Technology, Faculty of Sciences, International Agro-Food Campus of Excellence (CeiA3), University of Cádiz, Pol. Río San Pedro s/n, Puerto Real, Spain.
authors.forename: C
authors.initials: C
authors.lastname: Marzo
authors.affiliation: Department of Chemical Engineering and Food Technology, Faculty of Sciences, International Agro-Food Campus of Excellence (CeiA3), University of Cádiz, Pol. Río San Pedro s/n, Puerto Real, Spain.
authors.forename: I
authors.initials: I
authors.lastname: Caro
authors.affiliation: Department of Chemical Engineering and Food Technology, Faculty of Sciences, International Agro-Food Campus of Excellence (CeiA3), University of Cádiz, Pol. Río San Pedro s/n, Puerto Real, Spain.
authors.forename: I
authors.initials: I
authors.lastname: de Ory
authors.affiliation: Department of Chemical Engineering and Food Technology, Faculty of Sciences, International Agro-Food Campus of Excellence (CeiA3), University of Cádiz, Pol. Río San Pedro s/n, Puerto Real, Spain.
authors.forename: A
authors.initials: A
authors.lastname: Blandino
mesh_terms: D027461:Beta vulgaris / Q000737:chemistry / Q000378:metabolism; D018533:Biomass; D050260:Carbohydrate Metabolism; D002241:Carbohydrates / Q000032:analysis / Q000737:chemistry; D005285:Fermentation; D006868:Hydrolysis; D012037:Refuse Disposal / Q000379:methods*; D001617:beta-Glucosidase / Q000378:metabolism
chemical_list: D002241:Carbohydrates; D001617:beta-Glucosidase
keywords: Bioethanol; Enzyme hydrolysis; Fermentation; Lactic acid; Sugar beet cossettes