title: Curcumin Inhibits Transforming Growth Factor β Induced Differentiation of Mouse Lung Fibroblasts to Myofibroblasts.
abstract: Transforming growth factor β (TGF-β) induced differentiation of lung fibroblasts to myofibroblasts is a key event in the pathogenesis of pulmonary fibrosis. This study aimed to evaluate the effect of curcumin on TGF-β induced differentiation of lung fibroblasts to myofibroblasts and explore the underlying mechanism. Mouse lung fibroblasts were cultured and treated with TGF-β2 and curcumin or rosiglitazone. Cell vitality was examined by MTT assay. The secretion of collagen-1 was assessed by ELISA. α smooth muscle actin (α-SMA) was visualized by immunofluorescence technique. The expression of peroxisome proliferator activated receptor γ (PPAR-γ) and platelet derived growth factor R β (PDGFR-β) was detected by PCR and Western blot analysis. We found that curcumin and rosiglitazone inhibited the proliferation and TGF-β induced differentiation of mouse lung fibroblasts. In addition, curcumin and rosiglitazone inhibited collagen-1 secretion and α-SMA expression in mouse lung fibroblasts. Furthermore, curcumin and rosiglitazone upregulated PPAR-γ and downregulated PDGFR-β expression in mouse lung fibroblasts. In conclusion, our study reveals novel mechanism by which curcumin inhibits TGF-β2 driven differentiation of lung fibroblasts to myofibroblasts. Curcumin could potentially be used for effective treatment of pulmonary fibrosis.
authors.affiliation: Department of Respiratory Medicine, Institute of Respiratory Diseases in Zunyi, The First People's Hospital of Zunyi, The Third Affiliated Hospital of Zunyi Medical College Zunyi, China.
authors.forename: Daishun
authors.initials: D
authors.lastname: Liu
authors.affiliation: Department of Respiratory Medicine, Institute of Respiratory Diseases in Zunyi, The First People's Hospital of Zunyi, The Third Affiliated Hospital of Zunyi Medical College Zunyi, China.
authors.forename: Ling
authors.initials: L
authors.lastname: Gong
authors.affiliation: Department of Respiratory Medicine, Institute of Respiratory Diseases in Zunyi, The First People's Hospital of Zunyi, The Third Affiliated Hospital of Zunyi Medical College Zunyi, China.
authors.forename: Honglan
authors.initials: H
authors.lastname: Zhu
authors.affiliation: Department of Respiratory Medicine, Institute of Respiratory Diseases in Zunyi, The First People's Hospital of Zunyi, The Third Affiliated Hospital of Zunyi Medical College Zunyi, China.
authors.forename: Shenglan
authors.initials: S
authors.lastname: Pu
authors.affiliation: Department of Respiratory Medicine, Institute of Respiratory Diseases in Zunyi, The First People's Hospital of Zunyi, The Third Affiliated Hospital of Zunyi Medical College Zunyi, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Wu
authors.affiliation: Department of Respiratory Medicine, Institute of Respiratory Diseases in Zunyi, The First People's Hospital of Zunyi, The Third Affiliated Hospital of Zunyi Medical College Zunyi, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.affiliation: Department of Respiratory Medicine, Institute of Respiratory Diseases in Zunyi, The First People's Hospital of Zunyi, The Third Affiliated Hospital of Zunyi Medical College Zunyi, China.
authors.forename: Guichuan
authors.initials: G
authors.lastname: Huang
keywords: curcumin; peroxisome proliferator activated receptor γ; platelet derived growth factor receptor β; pulmonary fibrosis; transforming growth factor β2