title: Role of ectonucleotide pyrophosphatase/phosphodiesterase 2 in the midline axis formation of zebrafish.
abstract: Lysophosphatidic acid (LPA) is a unique bioactive lysophospholipid that induces pleiotropic effects in various cell types and organisms by acting on its specific receptors. LPA is mainly synthetised extracellularly by the ectonucleotide pyrophosphatase/phosphodiesterase 2/autotaxin (enpp2). Altered LPA signalling is associated with embryonic abnormalities, suggesting critical roles for LPA during development. However, the role of LPA signalling during early embryogenesis is not well established. We demonstrate that enpp2/LPA signalling in the early zebrafish embryo results in altered axis and midline formation, defects in left right (L-R) patterning, ciliogenesis of the Kupffer's vesicle (KV), through the modulation of cell migration during gastrulation in a lpar1-3Rho/ROCK-dependant manner. Overall, this study demonstrates an essential role of enpp2/LPA signalling during early embryogenesis.
authors.affiliation: Australian Regenerative Medicine Institute, Building 75, Monash University, Australia.|Centre for Eye Research Australia, Royal Victorian Eye and Ear Hospital &Ophthalmology, the University of Melbourne, Department of Surgery, Australia.
authors.forename: Frisca
authors.initials: F
authors.lastname: Frisca
authors.affiliation: Australian Regenerative Medicine Institute, Building 75, Monash University, Australia.
authors.forename: Daniel
authors.initials: D
authors.lastname: Colquhoun
authors.affiliation: Australian Regenerative Medicine Institute, Building 75, Monash University, Australia.|Department of Neurobiology, Tel-Aviv University, Israel.
authors.forename: Yona
authors.initials: Y
authors.lastname: Goldshmit
authors.affiliation: Monash Biomedicine Discovery Institute Department of Anatomy and Developmental Biology, Biomedicine Discovery Institute, Monash University, Australia.
authors.forename: Minna-Liisa
authors.initials: ML
authors.lastname: Änkö
authors.affiliation: Centre for Eye Research Australia, Royal Victorian Eye and Ear Hospital &Ophthalmology, the University of Melbourne, Department of Surgery, Australia.
authors.forename: Alice
authors.initials: A
authors.lastname: Pébay
authors.affiliation: Australian Regenerative Medicine Institute, Building 75, Monash University, Australia.
authors.forename: Jan
authors.initials: J
authors.lastname: Kaslin
mesh_terms: D000818:Animals; D019521:Body Patterning; D002465:Cell Movement; D002923:Cilia / Q000378:metabolism; D004625:Embryo, Nonmammalian / Q000201:enzymology*; D047108:Embryonic Development / Q000235:genetics; D018507:Gene Expression Regulation, Developmental; D038081:Organogenesis; D010641:Phenotype; D010727:Phosphoric Diester Hydrolases / Q000235:genetics / Q000378:metabolism*; D015027:Zebrafish / Q000196:embryology* / Q000235:genetics / Q000378:metabolism*; D029961:Zebrafish Proteins / Q000235:genetics / Q000378:metabolism*; D020741:rho GTP-Binding Proteins / Q000378:metabolism; D054460:rho-Associated Kinases / Q000378:metabolism; D000099189:Lysophospholipase D
chemical_list: D010727:Phosphoric Diester Hydrolases; D029961:Zebrafish Proteins; D020741:rho GTP-Binding Proteins; D054460:rho-Associated Kinases; C000627169:enpp2 protein, zebrafish; D000099189:Lysophospholipase D