title: NDM-1- and OXA-163-producing Klebsiella pneumoniae isolates in Cairo, Egypt, 2012.
abstract: Here we describe carbapenem resistance determinants in two Klebsiella pneumoniae isolates recovered from two hospitalised patients in the same intensive care unit of a cancer hospital in Cairo, Egypt. PCR and sequencing were used to detect and characterise β-lactamase genes. Clonal relationships between the isolates were analysed by pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST). The first K. pneumoniae isolate carried the blaNDM-1gene and the second isolate carried the blaOXA-163gene. Both isolates co-expressed the extended-spectrum β-lactamase CTX-M-15. The two isolates belonged to different sequence types (STs), ST11 and ST16, respectively. No history of travel was established for the two patients. The first identification of NDM-1-producing K. pneumoniae in Egypt adds further evidence to the spread of NDM-1-producing Gram-negative micro-organisms in North Africa. The additional detection of blaOXA-163in a K. pneumoniae isolate confirms its endemic presence in a critical healthcare setting of this geographic area.
authors.affiliation: Department of Microbiology and Immunology, Faculty of Pharmacy, Helwan University, Cairo, Egypt.
authors.forename: Mohammed O
authors.initials: MO
authors.lastname: Abdelaziz
authors.affiliation: Department of Sciences for Health Promotion and Mother-Child Care 'G. D'Alessandro', University of Palermo, Via del Vespro 133, I-90127 Palermo, Italy.
authors.forename: Celestino
authors.initials: C
authors.lastname: Bonura
authors.affiliation: Department of Sciences for Health Promotion and Mother-Child Care 'G. D'Alessandro', University of Palermo, Via del Vespro 133, I-90127 Palermo, Italy.
authors.forename: Aurora
authors.initials: A
authors.lastname: Aleo
authors.affiliation: Department of Sciences for Health Promotion and Mother-Child Care 'G. D'Alessandro', University of Palermo, Via del Vespro 133, I-90127 Palermo, Italy.
authors.forename: Teresa
authors.initials: T
authors.lastname: Fasciana
authors.affiliation: Department of Sciences for Health Promotion and Mother-Child Care 'G. D'Alessandro', University of Palermo, Via del Vespro 133, I-90127 Palermo, Italy. Electronic address: caterina.mammina@unipa.it.
authors.forename: Caterina
authors.initials: C
authors.lastname: Mammina
keywords: Antimicrobial drug resistance; Carbapenemase; Klebsiella pneumoniae; NDM-1; OXA-163