title: Comparative Analysis of TwoHelicobacter pyloriStrains using Genomics and Mass Spectrometry-Based Proteomics.
abstract: Helicobacter pylori, a gastroenteric pathogen believed to have co-evolved with humans over 100,000 years, shows significant genetic variability. This motivates the study of differentH. pyloristrains and the diseases they cause in order to identify determinants for disease evolution. In this study, we used proteomics tools to compare twoH. pyloristrains. Nic25_A was isolated in Nicaragua from a patient with intestinal metaplasia, and P12 was isolated in Europe from a patient with duodenal ulcers. Differences in the abundance of surface proteins between the two strains were determined with two mass spectrometry-based methods, label-free quantification (MaxQuant) or the use of tandem mass tags (TMT). Each approach used a lipid-based protein immobilization (LPITM) technique to enrich peptides of surface proteins. Using the MaxQuant software, we found 52 proteins that differed significantly in abundance between the two strains (up- or downregulated by a factor of 1.5); with TMT, we found 18 proteins that differed in abundance between the strains. Strain P12 had a higher abundance of proteins encoded by thecagpathogenicity island, while levels of the acid response regulator ArsR and its regulatory targets (KatA, AmiE, and proteins involved in urease production) were higher in strain Nic25_A. Our results show that differences in protein abundance betweenH. pyloristrains can be detected with proteomic approaches; this could have important implications for the study of disease progression.
authors.affiliation: Nanoxis Consulting ABGothenburg, Sweden; Department of Infectious Diseases, Sahlgrenska Academy, University of GothenburgGothenburg, Sweden.
authors.forename: Roger
authors.initials: R
authors.lastname: Karlsson
authors.affiliation: Department of Microbiology and Immunology, University of GothenburgGothenburg, Sweden; Department of Biology and Biological Engineering, Chalmers University of TechnologyGothenburg, Sweden.
authors.forename: Kaisa
authors.initials: K
authors.lastname: Thorell
authors.affiliation: Department of Biology and Biological Engineering, Chalmers University of Technology Gothenburg, Sweden.
authors.forename: Shaghayegh
authors.initials: S
authors.lastname: Hosseini
authors.affiliation: Proteomics Core Facility, Sahlgrenska Academy, University of Gothenburg Gothenburg, Sweden.
authors.forename: Diarmuid
authors.initials: D
authors.lastname: Kenny
authors.affiliation: Proteomics Core Facility, Sahlgrenska Academy, University of Gothenburg Gothenburg, Sweden.
authors.forename: Carina
authors.initials: C
authors.lastname: Sihlbom
authors.affiliation: Department of Microbiology and Immunology, University of Gothenburg Gothenburg, Sweden.
authors.forename: Åsa
authors.initials: Å
authors.lastname: Sjöling
authors.affiliation: Nanoxis Consulting AB Gothenburg, Sweden.
authors.forename: Anders
authors.initials: A
authors.lastname: Karlsson
authors.affiliation: Department of Biology and Biological Engineering, Chalmers University of TechnologyGothenburg, Sweden; Department of Biomedical Informatics, College of Medicine, University of Arkansas for Medical Sciences, Little RockAR, USA.
authors.forename: Intawat
authors.initials: I
authors.lastname: Nookaew
keywords: Genomics; Helicobacter pylori; MaxQuant; TMT; protein abundance; proteomics