title: Improved Production of Thermostable Cellulase from Thermoascus aurantiacus RCKK by Fermentation Bioprocessing and Its Application in the Hydrolysis of Office Waste Paper, Algal Pulp, and Biologically Treated Wheat Straw.
abstract: Thermostable cellulases have wide variety of applications and distinctive advantages, but their low titer becomes the hurdle in their commercialization. In the present work, an assessment of optimum levels of significant factors (temperature, moisture ratio, inoculum size, and ammonium sulfate) and the effect of their interactions on production of thermostable CMCase, FPase, and β-glucosidase by Thermoascus aurantiacus RCKK under solid-state fermentation (SSF) was carried out using central composite design (CCD) of response surface methodology (RSM). The study revealed 33, 13, and 8 % improvement in FPase, CMCase, and β-glucosidase production, respectively. Moreover, crude cellulase from T. aurantiacus RCKK efficiently hydrolyzed office waste paper, algal pulp (Gracillaria verulosa), and biologically treated wheat straw at 60 °C with sugar release of about 830 mg/ml, 285 mg/g, and 260 mg/g of the substrate, respectively. The thermostable enzyme from T. aurantiacus RCKK holds potential to be used in biofuel industry.
authors.affiliation: Lignocellulose Biotechnology Laboratory, Department of Microbiology, University of Delhi South Campus, Benito Juarez Road, New Delhi, 110021, India.|Central University of Haryana, Mahendergarh, Haryana, 123029, India.
authors.forename: Kavish Kumar
authors.initials: KK
authors.lastname: Jain
authors.affiliation: Lignocellulose Biotechnology Laboratory, Department of Microbiology, University of Delhi South Campus, Benito Juarez Road, New Delhi, 110021, India.|Central University of Haryana, Mahendergarh, Haryana, 123029, India.
authors.forename: Sandeep
authors.initials: S
authors.lastname: Kumar
authors.affiliation: Lignocellulose Biotechnology Laboratory, Department of Microbiology, University of Delhi South Campus, Benito Juarez Road, New Delhi, 110021, India.|Central University of Haryana, Mahendergarh, Haryana, 123029, India.
authors.forename: Deepa
authors.initials: D
authors.lastname: Deswal
authors.affiliation: Lignocellulose Biotechnology Laboratory, Department of Microbiology, University of Delhi South Campus, Benito Juarez Road, New Delhi, 110021, India. kuhad85@gmail.com.|Central University of Haryana, Mahendergarh, Haryana, 123029, India. kuhad85@gmail.com.
authors.forename: Ramesh Chander
authors.initials: RC
authors.lastname: Kuhad
mesh_terms: D001673:Biodegradation, Environmental; D002480:Cellulase / Q000096:biosynthesis* / Q000737:chemistry*; D004795:Enzyme Stability; D056890:Eukaryota / Q000737:chemistry; D006358:Hot Temperature; D007220:Industrial Waste / Q000517:prevention & control*; D010209:Paper; D018547:Plant Stems / Q000737:chemistry; D012037:Refuse Disposal / Q000379:methods; D013045:Species Specificity; D055327:Thermoascus / Q000145:classification / Q000201:enzymology*; D014908:Triticum / Q000737:chemistry*
chemical_list: D007220:Industrial Waste; D002480:Cellulase
keywords: Algal biomass; Biological pretreatment; Cellulase; Office waste paper; Saccharification