title: Recombinant Haemonchus contortus 24 kDa excretory/secretory protein (rHcES-24) modulate the immune functions of goat PBMCs in vitro.
abstract: A 24 kDa protein is one of the important components in Haemonchus contortus (barber pole worm) excretory/secretory products (HcESPs), which was shown to have important antigenic function. However, little is known about the immunomodulatory effects of this proteinon host cell. In the present study gene encoding 24kDa excretory/secretory protein (HcES-24) was cloned. The recombinant protein of HcES-24 (rHcES-24) was expressed in a histidine-tagged fusion protein soluble form in Escherichia coli. Binding activity of rHcES-24 to goat PBMCs was confirmed by immunofluorescence assay (IFA) and its immunomudulatory effect on cytokine secretion, cell proliferation, cell migration and nitric oxide production were observed by co-incubation of rHcES-24. IFA results revealed that rHcES-24 could bind to the PBMCs. The interaction of rHcES-24 increased the production of IL4, IL10, IL17 and cell migration in dose dependent manner. However, rHcES-24 treatment significantly suppressed the production of IFNγ, proliferation of the PBMC and Nitric oxide (NO) production. Our findings showed that the rHcES-24 played important regulatory effects on the goat PBMCs.
authors.affiliation: College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, PR China.|Department of Veterinary Parasitology, Sindh Agriculture University Tandojam, Pakistan.
authors.forename: Javaid Ali
authors.initials: JA
authors.lastname: Gadahi
authors.affiliation: College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, PR China.
authors.forename: Baojie
authors.initials: B
authors.lastname: Li
authors.affiliation: College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, PR China.
authors.forename: Muhammad
authors.initials: M
authors.lastname: Ehsan
authors.affiliation: College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, PR China.
authors.forename: Shuai
authors.initials: S
authors.lastname: Wang
authors.affiliation: College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, PR China.
authors.forename: Zhenchao
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, PR China.
authors.forename: Yujian
authors.initials: Y
authors.lastname: Wang
authors.affiliation: College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, PR China.
authors.forename: Muhammad Waqqas
authors.initials: MW
authors.lastname: Hasan
authors.affiliation: College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, PR China.
authors.forename: Ruofeng
authors.initials: R
authors.lastname: Yan
authors.affiliation: College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, PR China.
authors.forename: Xiaokai
authors.initials: X
authors.lastname: Song
authors.affiliation: College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, PR China.
authors.forename: Lixin
authors.initials: L
authors.lastname: Xu
authors.affiliation: College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, PR China.
authors.forename: Xiangrui
authors.initials: X
authors.lastname: Li
mesh_terms: D000818:Animals; D049109:Cell Proliferation; D002478:Cells, Cultured; D003001:Cloning, Molecular; D016207:Cytokines / Q000276:immunology / Q000378:metabolism; D006041:Goats / Q000276:immunology* / Q000378:metabolism / Q000469:parasitology; D006189:Haemonchus / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D015801:Helminth Proteins / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D006790:Host-Parasite Interactions; D007963:Leukocytes, Mononuclear / Q000276:immunology* / Q000378:metabolism / Q000469:parasitology; D009569:Nitric Oxide / Q000276:immunology / Q000378:metabolism; D010802:Phylogeny; D011994:Recombinant Proteins / Q000276:immunology / Q000378:metabolism; D020539:Sequence Analysis, Protein
chemical_list: D016207:Cytokines; D015801:Helminth Proteins; D011994:Recombinant Proteins; D009569:Nitric Oxide
keywords: 24 kDa; ESP; H. contortus; Immune response; Immunity; Immunology and Microbiology Section; cell migration; cytokines; proliferation