title: Remarkable alterations of Nav1.6 in reactive astrogliosis during epileptogenesis.
abstract: Voltage-gated sodium channels (VGSCs) play a vital role in controlling neuronal excitability. Nav1.6 is the most abundantly expressed VGSCs subtype in the adult central nervous system and has been found to contribute to facilitate the hyperexcitability of neurons after electrical induction of status epilepticus (SE). To clarify the exact expression patterns of Nav1.6 during epileptogenesis, we examined the expression of Nav1.6 at protein and mRNA levels in two distinct animal models of temporal lobe epilepsy (TLE) including a post-SE model induced by kainic acid (KA) intrahippocampal injection and a kindling model evoked by pentylenetetrazole (PTZ). A prominent, seizure intensity-dependent increase of Nav1.6 expression in reactive astrocytes was observed in ipsilateral hippocampus of post-SE rats, reaching the peak at 21 days after SE, a time point during the latent stage of epileptogenesis. However, Nav1.6 with low expression level was selectively expressed in the hippocampal neurons rather than astrocytes in PTZ-kindled animals. This seizure-related increase of a VGSCs subtype in reactive astrocytes after SE may represent a new mechanism for signal communication between neuron and glia in the course of epileptogenesis, facilitating the neuronal hyperexcitability.
authors.affiliation: Laboratory of Neuropharmacology and Neurotoxicology, School of Life Science, Shanghai University, Nanchen Road 333, Shanghai, 200444, China.
authors.forename: Hongyan
authors.initials: H
authors.lastname: Zhu
authors.affiliation: Laboratory of Neuropharmacology and Neurotoxicology, School of Life Science, Shanghai University, Nanchen Road 333, Shanghai, 200444, China.
authors.forename: Yuxiao
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: Laboratory of Neuropharmacology and Neurotoxicology, School of Life Science, Shanghai University, Nanchen Road 333, Shanghai, 200444, China.
authors.forename: Hao
authors.initials: H
authors.lastname: Wu
authors.affiliation: Laboratory of Neuropharmacology and Neurotoxicology, School of Life Science, Shanghai University, Nanchen Road 333, Shanghai, 200444, China.
authors.forename: Nan
authors.initials: N
authors.lastname: Jiang
authors.affiliation: Laboratory of Neuropharmacology and Neurotoxicology, School of Life Science, Shanghai University, Nanchen Road 333, Shanghai, 200444, China.
authors.forename: Ziyi
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Laboratory of Neuropharmacology and Neurotoxicology, School of Life Science, Shanghai University, Nanchen Road 333, Shanghai, 200444, China.
authors.forename: Weide
authors.initials: W
authors.lastname: Lin
authors.affiliation: Laboratory of Neuropharmacology and Neurotoxicology, School of Life Science, Shanghai University, Nanchen Road 333, Shanghai, 200444, China.
authors.forename: Jiahui
authors.initials: J
authors.lastname: Jin
authors.affiliation: Laboratory of Neuropharmacology and Neurotoxicology, School of Life Science, Shanghai University, Nanchen Road 333, Shanghai, 200444, China.
authors.forename: Yonghua
authors.initials: Y
authors.lastname: Ji
mesh_terms: D000818:Animals; D001253:Astrocytes / Q000378:metabolism*; D003292:Convulsants / Q000633:toxicity; D004195:Disease Models, Animal; D004833:Epilepsy, Temporal Lobe / Q000209:etiology / Q000235:genetics* / Q000378:metabolism*; D006624:Hippocampus / Q000378:metabolism; D006801:Humans; D007608:Kainic Acid / Q000633:toxicity; D007696:Kindling, Neurologic / Q000235:genetics / Q000378:metabolism; D008297:Male; D062557:NAV1.6 Voltage-Gated Sodium Channel / Q000235:genetics* / Q000378:metabolism*; D009474:Neurons / Q000378:metabolism; D010433:Pentylenetetrazole / Q000633:toxicity; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D012640:Seizures / Q000235:genetics / Q000378:metabolism; D013226:Status Epilepticus / Q000139:chemically induced / Q000150:complications; D013997:Time Factors
chemical_list: D003292:Convulsants; D062557:NAV1.6 Voltage-Gated Sodium Channel; D012333:RNA, Messenger; C507811:Scn8a protein, rat; D007608:Kainic Acid; D010433:Pentylenetetrazole