title: Improved proliferation of antigen-specific cytolytic T lymphocytes using a multimodal nanovaccine.
abstract: The present study investigated the immunoenhancing property of our newly designed nanovaccine, that is, its ability to induce antigen-specific immunity. This study also evaluated the synergistic effect of a novel compound PBS-44, an α-galactosylceramide analog, in boosting the immune response induced by our nanovaccine. The nanovaccine was prepared by encapsulating ovalbumin (ova) and an adjuvant within the poly(lactic-co-glycolic acid) nanoparticles. Quantitative analysis of our study data showed that the encapsulated vaccine was physically and biologically stable; the core content of our nanovaccine was found to be released steadily and slowly, and nearly 90% of the core content was slowly released over the course of 25 days. The in vivo immunization studies exhibited that the nanovaccine induced stronger and longer immune responses compared to its soluble counterpart. Similarly, intranasal inhalation of the nanovaccine induced more robust antigen-specific CD8+T cell response than intraperitoneal injection of nanovaccine.
authors.affiliation: Institute of Imaging Science, Vanderbilt University School of Medicine; Department of Radiology and Radiological Sciences.
authors.forename: Bo
authors.initials: B
authors.lastname: Li
authors.affiliation: Institute of Imaging Science, Vanderbilt University School of Medicine.
authors.forename: Michael
authors.initials: M
authors.lastname: Siuta
authors.affiliation: Institute of Imaging Science, Vanderbilt University School of Medicine; Department of Radiology and Radiological Sciences.
authors.forename: Vanessa
authors.initials: V
authors.lastname: Bright
authors.affiliation: Department of Chemistry, Vanderbilt University; Vanderbilt Institute of Nanoscale Science and Engineering.
authors.forename: Dmitry
authors.initials: D
authors.lastname: Koktysh
authors.affiliation: Vanderbilt Flow Cytometry Shared Resource, Vanderbilt University.
authors.forename: Brittany K
authors.initials: BK
authors.lastname: Matlock
authors.affiliation: Institute of Imaging Science, Vanderbilt University School of Medicine.
authors.forename: Megan E
authors.initials: ME
authors.lastname: Dumas
authors.affiliation: Institute of Imaging Science, Vanderbilt University School of Medicine.
authors.forename: Meiying
authors.initials: M
authors.lastname: Zhu
authors.affiliation: Institute of Imaging Science, Vanderbilt University School of Medicine.
authors.forename: Alex
authors.initials: A
authors.lastname: Holt
authors.affiliation: Department of Chemistry, Vanderbilt University; Vanderbilt Institute of Chemical Biology.
authors.forename: Donald
authors.initials: D
authors.lastname: Stec
authors.affiliation: Department of Chemistry and Biochemistry, Brigham Young University.
authors.forename: Shenglou
authors.initials: S
authors.lastname: Deng
authors.affiliation: Department of Chemistry and Biochemistry, Brigham Young University.
authors.forename: Paul B
authors.initials: PB
authors.lastname: Savage
authors.affiliation: Department of Pathology, Microbiology and Immunology, Vanderbilt University; Veterans Administration Tennessee Valley Healthcare System.
authors.forename: Sebastian
authors.initials: S
authors.lastname: Joyce
authors.affiliation: Institute of Imaging Science, Vanderbilt University School of Medicine; Department of Radiology and Radiological Sciences; Vanderbilt Institute of Chemical Biology; Department of Biomedical Engineering; Vanderbilt Ingram Cancer Center; Vanderbilt Brain Institute, Vanderbilt University, Nashville, TN, USA.
authors.forename: Wellington
authors.initials: W
authors.lastname: Pham
mesh_terms: D000276:Adjuvants, Immunologic / Q000494:pharmacology; D000281:Administration, Intranasal; D000818:Animals; D016923:Cell Death / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects / Q000502:physiology; D003713:Dendritic Cells / Q000276:immunology; D005699:Galactosylceramides / Q000737:chemistry / Q000494:pharmacology*; D007114:Immunization; D007274:Injections, Intraperitoneal; D019344:Lactic Acid / Q000737:chemistry; D051379:Mice; D008810:Mice, Inbred C57BL; D018625:Microscopy, Atomic Force; D053758:Nanoparticles / Q000737:chemistry; D010047:Ovalbumin / Q000737:chemistry / Q000276:immunology; D011100:Polyglycolic Acid / Q000737:chemistry; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D013601:T-Lymphocytes / Q000187:drug effects* / Q000276:immunology; D014612:Vaccines / Q000008:administration & dosage* / Q000737:chemistry / Q000276:immunology / Q000494:pharmacology*
chemical_list: D000276:Adjuvants, Immunologic; D005699:Galactosylceramides; C000614458:PBS-44; D014612:Vaccines; C493154:alpha-galactosylceramide; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; D010047:Ovalbumin
keywords: GalCer; dendritic cells; inhalable vaccine; nanovaccine