title: Rapid detection of Salmonella in raw chicken breast using real-time PCR combined with immunomagnetic separation and whole genome amplification.
abstract: We presented the first attempt to combine immunomagnetic separation (IMS), whole genome amplification by multiple displacement amplification (MDA) and real-time PCR for detecting a bacterial pathogen in a food sample. This method was effective in enabling real-time PCR detection of low levels of Salmonella enterica Serotype Enteritidis (SE) (∼10 CFU/g) in raw chicken breast without culture enrichment. In addition, it was able to detect refrigeration-stressed SE cells at lower concentrations (∼0.1 CFU/g) in raw chicken breast after a 4-h culture enrichment, shortening the detection process from days to hours and displaying no statistical difference in detection rate in comparison with a culture-based detection method. By substantially improving performance in SE detection over conventional real-time PCR, we demonstrated the potential of IMS-MDA real-time PCR as a rapid, sensitive and affordable method for detecting Salmonella in food.
authors.affiliation: Center for Food Safety, Department of Food Science and Technology, University of Georgia, 1109 Experiment St, Griffin, GA, 30223, USA.
authors.forename: Ji-Yeon
authors.initials: JY
authors.lastname: Hyeon
authors.affiliation: Center for Food Safety, Department of Food Science and Technology, University of Georgia, 1109 Experiment St, Griffin, GA, 30223, USA. Electronic address: xdeng@uga.edu.
authors.forename: Xiangyu
authors.initials: X
authors.lastname: Deng
mesh_terms: D000818:Animals; D058491:Bacterial Load; D004269:DNA, Bacterial; D005516:Food Microbiology; D016680:Genome, Bacterial; D018189:Immunomagnetic Separation / Q000379:methods; D021141:Nucleic Acid Amplification Techniques; D011200:Poultry / Q000382:microbiology*; D060888:Real-Time Polymerase Chain Reaction / Q000191:economics / Q000379:methods*; D012477:Salmonella enteritidis / Q000235:genetics* / Q000254:growth & development / Q000276:immunology / Q000302:isolation & purification*; D012680:Sensitivity and Specificity
chemical_list: D004269:DNA, Bacterial
keywords: Chicken; Detection; IMS; MDA; Real-time PCR; Salmonella; Whole genome amplification