title: Loss of ZG16 is regulated by miR-196a and contributes to stemness and progression of colorectal cancer.
abstract: Colorectal cancer (CRC) is one of the most common malignant tumour and the leading cause of cancer-related mortality worldwide. Clarification of the mechanism that underlies CRC tumorigenesis and progression therefore is urgently needed ffor developing novel therapies. Through analysis of The Cancer Genome Atlas (TCGA) dataset, we identified an interesting gene, ZG16, which is significantly decreased in CRC samples compared to adjacent non-tumor tissues and associated with prognosis of patients. We found that the expression of ZG16 correlated with CRC related genes which were regulated by APC/CTNNB1 pathway. Interestingly, the expression of ZG16 was negatively correlated with CRC stem cell marker, LGR5. Overexpression of ZG16 significantly inhibits growth and sphere formation of stem-like CRC cells. Moreover, we also identified an upstream regulator of ZG16, miR-196a, which was significantly overexpressed in CRC and promotes cell growth and stemness. In conclusion, this study demonstrated that loss of ZG16 is regulated by miR-196a and contributes to stemness and progression of CRC, which may provide a promising therapeutic strategy for advanced CRCs.
authors.affiliation: Department of Oncology, Henan Cancer Hospital, The Affiliated Cancer Hospital of Zhengzhou University, Zhengzhou 450008, China.
authors.forename: Xiaobing
authors.initials: X
authors.lastname: Chen
authors.affiliation: Department of Colorectal Surgery, Xinhua Hospital, Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai 200092, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Du
authors.affiliation: Department of General Surgery, The First Affiliated Hospital of Xi'An Jiaotong University, Xi'An 710061, China.
authors.forename: Junjun
authors.initials: J
authors.lastname: She
authors.affiliation: Department of Oncology, Henan Cancer Hospital, The Affiliated Cancer Hospital of Zhengzhou University, Zhengzhou 450008, China.
authors.forename: Liang
authors.initials: L
authors.lastname: Cao
authors.affiliation: Department of Gastroenterology, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an 710061, China.
authors.forename: Yingchao
authors.initials: Y
authors.lastname: Li
authors.affiliation: Department of General Surgery, The First Affiliated Hospital of Xi'An Jiaotong University, Xi'An 710061, China.
authors.forename: Hongping
authors.initials: H
authors.lastname: Xia
mesh_terms: D020413:3' Untranslated Regions / Q000235:genetics; D000818:Animals; D049109:Cell Proliferation / Q000235:genetics; D015179:Colorectal Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D018450:Disease Progression; D015972:Gene Expression Regulation, Neoplastic; D019073:HT29 Cells; D006801:Humans; D037102:Lectins / Q000235:genetics* / Q000378:metabolism; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D035683:MicroRNAs / Q000235:genetics*; D014411:Neoplastic Stem Cells / Q000378:metabolism*; D043562:Receptors, G-Protein-Coupled / Q000235:genetics; D015398:Signal Transduction / Q000235:genetics; D014183:Transplantation, Heterologous
chemical_list: D020413:3' Untranslated Regions; C116884:LGR5 protein, human; D037102:Lectins; C542543:MIRN196 microRNA, human; D035683:MicroRNAs; D043562:Receptors, G-Protein-Coupled; C519401:ZG16 protein, human
keywords: Colorectal cancer; LGR5; ZG16; miR-196a; stemness