title: Construction and Screening of Marine Metagenomic Large Insert Libraries.
abstract: The marine environment covers more than 70 % of the world's surface. Marine microbial communities are highly diverse and have evolved during extended evolutionary processes of physiological adaptations under the influence of a variety of ecological conditions and selection pressures. They harbor an enormous diversity of microbes with still unknown and probably new physiological characteristics. In the past, marine microbes, mostly bacteria of microbial consortia attached to marine tissues of multicellular organisms, have proven to be a rich source of highly potent bioactive compounds, which represent a considerable number of drug candidates. However, to date, the biodiversity of marine microbes and the versatility of their bioactive compounds and metabolites have not been fully explored. This chapter describes sampling in the marine environment, construction of metagenomic large insert libraries from marine habitats, and exemplarily one function based screen of metagenomic clones for identification of quorum quenching activities.
authors.affiliation: Institut für Allgemeine Mikrobiologie, Christian-Albrechts-Universität zu Kiel, Am Botanischen Garten 1-9, 24118, Kiel, Germany.
authors.forename: Nancy
authors.initials: N
authors.lastname: Weiland-Bräuer
authors.affiliation: Institut für Allgemeine Mikrobiologie, Christian-Albrechts-Universität zu Kiel, Am Botanischen Garten 1-9, 24118, Kiel, Germany.
authors.forename: Daniela
authors.initials: D
authors.lastname: Langfeldt
authors.affiliation: Institut für Allgemeine Mikrobiologie, Christian-Albrechts-Universität zu Kiel, Am Botanischen Garten 1-9, 24118, Kiel, Germany. rschmitz@ifam.uni-kiel.de.
authors.forename: Ruth A
authors.initials: RA
authors.lastname: Schmitz
mesh_terms: D059001:Aquatic Organisms / Q000145:classification / Q000235:genetics*; D015723:Gene Library; D005822:Genetic Vectors; D059014:High-Throughput Nucleotide Sequencing; D054892:Metagenome; D056186:Metagenomics / Q000379:methods; D016254:Mutagenesis, Insertional; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012623:Seawater / Q000382:microbiology; D014871:Water Microbiology
chemical_list: D012336:RNA, Ribosomal, 16S
keywords: 16S rDNA phylogenetic analysis; Construction of fosmid libraries; Function-based screen; Isolation of metagenomic DNA; Quorum quenching