title: Differential pulmonic NK and NKT cell responses in Schistosoma japonicum-infected mice.
abstract: Natural killer cells (NK cells) and natural killer T cells (NKT cells) play a role in anti-infection, anti-tumor, transplantation immunity, and autoimmune regulation. However, the role of NK and NKT cells during Schistosoma japonicum (S. japonicum) infection has not been widely reported, especially regarding lung infections. The aim of this study was to research the NK and NKT cell response to S. japonicum infection in the lungs of mice. Using immunofluorescent histological analysis, NK and NKT cells were found near pulmonary granulomas. Moreover, flow cytometry revealed that the percentage and number of pulmonic NK cells in S. japonicum-infected mice were significantly increased (P < 0.05). However, the percentage and cell number of NKT cells were decreased compared to those of normal mice (P < 0.05). The expression of CD69 on pulmonic NK and NKT cells was increased after infection (P < 0.05), and CD25 expression increased only on NKT cells (P < 0.05). Intracellular cytokine staining showed a higher percentage of IFN-γ+and lower percentage of IL-5+pulmonic NK cells (P < 0.05) compared to controls. However, the percentage of IL-17+, IL-10+, and IL-5+pulmonic NKT cells significantly increased (P < 0.05). Additionally, there was a significant decrease in NKG2A/C/E (CD94) expression and an increase of NKG2D (CD314) expression on pulmonic NKT cells (P < 0.05), which might serve as a mechanism for NKT cell activation during S. japonicum infection.
authors.affiliation: Guangdong Provincial Key Laboratory of Allergy and Clinical Immunology, Institute of Immunology, Guangzhou Medical University, 511436, Guangzhou, China.
authors.forename: Hefei
authors.initials: H
authors.lastname: Cha
authors.affiliation: Department of Radiation Oncology, Nanfang Hospital, Southern Medical University, Guangdong, 510515, China.|Department of Radiation Oncology, Zhongshan Hospital, Xiamen University, Xiamen, Fujian, 361004, China.
authors.forename: Wenjuan
authors.initials: W
authors.lastname: Qin
authors.affiliation: Guangdong Provincial Key Laboratory of Allergy and Clinical Immunology, Institute of Immunology, Guangzhou Medical University, 511436, Guangzhou, China.
authors.forename: Quan
authors.initials: Q
authors.lastname: Yang
authors.affiliation: Guangdong Provincial Key Laboratory of Allergy and Clinical Immunology, Institute of Immunology, Guangzhou Medical University, 511436, Guangzhou, China.
authors.forename: Hongyan
authors.initials: H
authors.lastname: Xie
authors.affiliation: Guangdong Provincial Key Laboratory of Allergy and Clinical Immunology, Institute of Immunology, Guangzhou Medical University, 511436, Guangzhou, China.
authors.forename: Jiale
authors.initials: J
authors.lastname: Qu
authors.affiliation: Guangdong Provincial Key Laboratory of Allergy and Clinical Immunology, Institute of Immunology, Guangzhou Medical University, 511436, Guangzhou, China.
authors.forename: Mei
authors.initials: M
authors.lastname: Wang
authors.affiliation: Guangdong Provincial Key Laboratory of Allergy and Clinical Immunology, Institute of Immunology, Guangzhou Medical University, 511436, Guangzhou, China.
authors.forename: Daixiong
authors.initials: D
authors.lastname: Chen
authors.affiliation: Department of Medical Genetics and Cell Biology, School of Basic Sciences, Guangzhou Medical University, 511436, Guangzhou, China.
authors.forename: Fang
authors.initials: F
authors.lastname: Wang
authors.affiliation: Affiliated Xiamen Eye Center and Eye Institute of Xiamen University, 361001, Xiamen, China.
authors.forename: Nuo
authors.initials: N
authors.lastname: Dong
authors.affiliation: Department of Radiation Oncology, Nanfang Hospital, Southern Medical University, Guangdong, 510515, China. chenlhsmu@126.com.
authors.forename: Longhua
authors.initials: L
authors.lastname: Chen
authors.affiliation: Guangdong Provincial Key Laboratory of Allergy and Clinical Immunology, Institute of Immunology, Guangzhou Medical University, 511436, Guangzhou, China. hj165@sina.com.
authors.forename: Jun
authors.initials: J
authors.lastname: Huang
mesh_terms: D000818:Animals; D005260:Female; D006801:Humans; D007371:Interferon-gamma / Q000235:genetics; D016753:Interleukin-10 / Q000235:genetics / Q000276:immunology; D020381:Interleukin-17 / Q000235:genetics / Q000276:immunology; D015848:Interleukin-5 / Q000235:genetics / Q000276:immunology; D007694:Killer Cells, Natural / Q000276:immunology*; D008168:Lung / Q000276:immunology; D008213:Lymphocyte Activation / Q000276:immunology; D051379:Mice; D008810:Mice, Inbred C57BL; D055611:Natural Killer T-Cells / Q000276:immunology*; D012549:Schistosoma japonicum / Q000276:immunology / Q000502:physiology*; D012554:Schistosomiasis japonica / Q000235:genetics / Q000276:immunology* / Q000469:parasitology
chemical_list: D020381:Interleukin-17; D015848:Interleukin-5; D016753:Interleukin-10; D007371:Interferon-gamma
keywords: Cytokines; Lung; NK cells; NKT cells; Schistosoma japonicum