title: SPECtre: a spectral coherence--based classifier of actively translated transcripts from ribosome profiling sequence data.
abstract: BACKGROUND
  Active protein translation can be assessed and measured using ribosome profiling sequencing strategies. Prevailing analytical approaches applied to this technology make use of sequence fragment length profiling or reading frame occupancy enrichment to differentiate between active translation and background noise, however they do not consider additional characteristics inherent to the technology which limits their overall accuracy.
  
  
  RESULTS
  Here, we present an analytical tool that models the overall tri-nucleotide periodicity of ribosomal occupancy using a classifier based on spectral coherence. Our software, SPECtre, examines the relationship of normalized ribosome profiling read coverage over a rolling series of windows along a transcript relative to an idealized reference signal without the matched requirement of mRNA-Seq.
  
  
  CONCLUSIONS
  A comparison of SPECtre against previously published methods on existing data shows a marked improvement in accuracy for detecting active translation and exhibits overall high accuracy at a low false discovery rate. In addition, SPECtre performs comparably to a recently published method similarly based on spectral coherence, however with reduced runtime and memory requirements. SPECtre is available as an open source software package at https://github.com/mills-lab/spectreok .
authors.affiliation: Department of Computational Medicine and Bioinformatics, University of Michigan, Ann Arbor, MI, 48109, USA.
authors.forename: Sang Y
authors.initials: SY
authors.lastname: Chun
authors.affiliation: Department of Neurology, University of Michigan, Ann Arbor, MI, 48109, USA.
authors.forename: Caitlin M
authors.initials: CM
authors.lastname: Rodriguez
authors.affiliation: Department of Neurology, University of Michigan, Ann Arbor, MI, 48109, USA.|Veterans Affairs Medical Center, Ann Arbor, MI, 48105, USA.
authors.forename: Peter K
authors.initials: PK
authors.lastname: Todd
authors.affiliation: Department of Computational Medicine and Bioinformatics, University of Michigan, Ann Arbor, MI, 48109, USA. remills@umich.edu.|Department of Human Genetics, University of Michigan, Ann Arbor, MI, 48109, USA. remills@umich.edu.
authors.forename: Ryan E
authors.initials: RE
authors.lastname: Mills
mesh_terms: D000465:Algorithms; D020869:Gene Expression Profiling; D057809:HEK293 Cells; D006801:Humans; D016366:Open Reading Frames; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism*; D012270:Ribosomes / Q000235:genetics / Q000378:metabolism*; D017423:Sequence Analysis, RNA / Q000379:methods*; D012984:Software; D059467:Transcriptome / Q000235:genetics*
chemical_list: D012333:RNA, Messenger
keywords: Classification; Ribosome profiling; Spectral coherence; Translation