title: Pharmacokinetic studies of the recombinant chicken interferon-α in broiler chickens.
abstract: In this study, 24 male and female broiler chickens at 30-day-old were divided into three groups with 8 animals in each group. The animals were administered with recombinant chicken interferon-α (rChIFN-α) at a dose of 1.0 × 106IU/kg intravenously, intramuscularly or subcutaneously, respectively. Serum samples were collected at different time points post administration, and the titers of rChIFN-α in the blood were determined by cytopathic effect inhibition assay. The results showed that the pharmacokinetic characteristics of rChIFN-α by intramuscular injection and subcutaneous injection were fitted to one compartment open model, and the Tmaxwas 3.21 ± 0.79 hr and 3.95 ± 0.85 hr, respectively, and the elimination half-life (T1/2) was 6.20 ± 2.77 hr and 5.03 ± 3.70 hr, respectively. In contrast, the pharmacokinetics of rChIFN-α via intravenous injection was in line with the open model of two-compartment and was eliminated in the first order, and the elimination half-life (T1/2) was 4.61 ± 0.84 hr. In addition, compared with those in the intravenous group and the subcutaneous group, the bioavailability of rChIFN-α in the intramuscular group was 82.80%. In conclusion, rChIFN-α was rapidly absorbed and slowly eliminated after intramuscular administration of single dose of rChIFN-α aqueous formulations. Thus, rChIFN-α can be used as a commonly-used therapeutic agent.
authors.affiliation: Wuhu Overseas Students Pioneer Park, Wuhu, Anhui Province, 241000, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Zhao
authors.forename: Hai-Yang
authors.initials: HY
authors.lastname: Yu
authors.forename: Jun-Ling
authors.initials: JL
authors.lastname: Zhang
authors.forename: Xing-Man
authors.initials: XM
authors.lastname: Wang
authors.forename: Jin-Pei
authors.initials: JP
authors.lastname: Li
authors.forename: Tao
authors.initials: T
authors.lastname: Hu
authors.forename: Yong
authors.initials: Y
authors.lastname: Hu
authors.forename: Ming-Li
authors.initials: ML
authors.lastname: Wang
authors.forename: Yong-Zhou
authors.initials: YZ
authors.lastname: Shen
authors.forename: Jing-Dong
authors.initials: JD
authors.lastname: Xu
authors.forename: Guo-Xiang
authors.initials: GX
authors.lastname: Han
authors.forename: Jason
authors.initials: J
authors.lastname: Chen
mesh_terms: D000818:Animals; D002645:Chickens / Q000097:blood / Q000378:metabolism; D005260:Female; D007273:Injections, Intramuscular / Q000662:veterinary; D007275:Injections, Intravenous / Q000662:veterinary; D007279:Injections, Subcutaneous / Q000662:veterinary; D016898:Interferon-alpha / Q000008:administration & dosage / Q000097:blood / Q000493:pharmacokinetics*; D008297:Male; D011994:Recombinant Proteins / Q000493:pharmacokinetics
chemical_list: D016898:Interferon-alpha; D011994:Recombinant Proteins