title: Identification of Species of Nontuberculous Mycobacteria Clinical Isolates from 8 Provinces of China.
abstract: Pulmonary diseases caused by nontuberculousmycobacteria(NTM) are increasing in incidence and prevalence worldwide. In this study, we identified NTM species of the clinical isolates from 8 provinces in China, in order to preliminarily provide some basic scientific data in the different species and distribution of NTM related to pulmonary disease in China. A total of 523 clinical isolates from patients with tuberculosis (TB) diagnosed clinically from 2005 to 2012 were identified to the species using conventional and molecular methods, including multilocus PCR,rpoBandhsp65PCR-PRA,hsp65,rpoB, and16S-23Sinternal transcribed spacer region sequencing. The isolates were identified into 3 bacterium genera, including NTM,Gordonia bronchialis,andNocardia farcinica, and, for the 488 NTM isolates, 27 species were identified. For all the 27 species of NTM which were found to cause pulmonary infections in humans, the most prevalent species wasM. intracellulare, followed byM. aviumandM. abscessus. And seven other species were for the first time identified in patients with TB in China. NTM species identification is very important for distinguishing between tuberculosis and NTM pulmonary diseases, and the species diversity drives the creation of diverse and integrated identification methods with higher accuracy and efficacy.
authors.affiliation: State Key Laboratory for Infectious Disease Prevention and Control, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China.
authors.forename: Haican
authors.identifier: 0000-0002-9420-1211
authors.initials: H
authors.lastname: Liu
authors.affiliation: State Key Laboratory for Infectious Disease Prevention and Control, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China; Pathogenic Biology Institute, University of South China, Hengyang, Hunan 421001, China.
authors.forename: Lulu
authors.initials: L
authors.lastname: Lian
authors.affiliation: State Key Laboratory for Infectious Disease Prevention and Control, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Jiang
authors.affiliation: Fuzhou Pulmonary Hospital (Clinical Teaching Hospital of Fujian Medical University), Fuzhou, Fujian 350008, China.
authors.forename: Mingxiang
authors.initials: M
authors.lastname: Huang
authors.affiliation: Hunan Institute for Tuberculosis Control/Hunan Chest Hospital, Changsha, Hunan 410013, China.
authors.forename: Yunhong
authors.initials: Y
authors.lastname: Tan
authors.affiliation: State Key Laboratory for Infectious Disease Prevention and Control, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China.
authors.forename: Xiuqin
authors.identifier: 0000-0003-0084-8119
authors.initials: X
authors.lastname: Zhao
authors.affiliation: State Key Laboratory for Infectious Disease Prevention and Control, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China.
authors.forename: Jingrui
authors.initials: J
authors.lastname: Zhang
authors.affiliation: State Key Laboratory for Infectious Disease Prevention and Control, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China; Pathogenic Biology Institute, University of South China, Hengyang, Hunan 421001, China.
authors.forename: Qin
authors.initials: Q
authors.lastname: Yu
authors.affiliation: State Key Laboratory for Infectious Disease Prevention and Control, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China.
authors.forename: Jiao
authors.initials: J
authors.lastname: Liu
authors.affiliation: State Key Laboratory for Infectious Disease Prevention and Control, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China.
authors.forename: Haiyan
authors.initials: H
authors.lastname: Dong
authors.affiliation: State Key Laboratory for Infectious Disease Prevention and Control, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China.
authors.forename: Bing
authors.initials: B
authors.lastname: Lu
authors.affiliation: Pathogenic Biology Institute, University of South China, Hengyang, Hunan 421001, China.
authors.forename: Yimou
authors.identifier: 0000-0001-6883-1677
authors.initials: Y
authors.lastname: Wu
authors.affiliation: State Key Laboratory for Infectious Disease Prevention and Control, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China; Pathogenic Biology Institute, University of South China, Hengyang, Hunan 421001, China.
authors.forename: Kanglin
authors.identifier: 0000-0002-3997-0237
authors.initials: K
authors.lastname: Wan
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics; D018834:Chaperonin 60 / Q000235:genetics; D002681:China; D021903:DNA, Ribosomal Spacer / Q000235:genetics; D005843:Geography; D033601:Gordonia Bacterium / Q000235:genetics; D006801:Humans; D008171:Lung Diseases / Q000453:epidemiology / Q000382:microbiology; D058885:Multilocus Sequence Typing; D009161:Mycobacterium / Q000235:genetics*; D009165:Mycobacterium Infections, Nontuberculous / Q000453:epidemiology* / Q000382:microbiology*; D009615:Nocardia / Q000235:genetics; D009170:Nontuberculous Mycobacteria / Q000235:genetics* / Q000302:isolation & purification; D016133:Polymerase Chain Reaction; D015995:Prevalence; D015203:Reproducibility of Results; D013045:Species Specificity
chemical_list: D001426:Bacterial Proteins; D018834:Chaperonin 60; D021903:DNA, Ribosomal Spacer; C074119:heat-shock protein 65, Mycobacterium