title: Hyper-thermal acid hydrolysis and adsorption treatment of red seaweed, Gelidium amansii for butyric acid production with pH control.
abstract: Optimal hyper-thermal (HT) acid hydrolysis conditions for Gelidium amansii were determined to be 12% (w/v) seaweed slurry content and 144 mM H2SO4at 150 °C for 10 min. HT acid hydrolysis-treated G. amansii hydrolysates produced low concentrations of inhibitory compounds and adsorption treatment using 3% activated carbon. An adsorption time of 5 min was subsequently used to remove the inhibitory 5-hydroxymethylfurfural from the medium. A final maximum monosaccharide concentration of 44.6 g/L and 79.1% conversion from 56.4 g/L total fermentable monosaccharides with 120 g dw/L G. amansii slurry was obtained from HT acid hydrolysis, enzymatic saccharification, and adsorption treatment. This study demonstrates the potential for butyric acid production from G. amansii hydrolysates under non-pH-controlled as well as pH-controlled fermentation using Clostridium acetobutylicum KCTC 1790. The activated carbon treatment and pH-controlled fermentation showed synergistic effects and produced butyric acid at a concentration of 11.2 g/L after 9 days of fermentation.
authors.affiliation: Department of Biotechnology, Pukyong National University, Busan, 48517, Korea.
authors.forename: Chae Hun
authors.initials: CH
authors.lastname: Ra
authors.affiliation: Department of Biotechnology, Pukyong National University, Busan, 48517, Korea.
authors.forename: Gwi-Taek
authors.initials: GT
authors.lastname: Jeong
authors.affiliation: Department of Biotechnology, Pukyong National University, Busan, 48517, Korea. skkim@pknu.ac.kr.
authors.forename: Sung-Koo
authors.initials: SK
authors.lastname: Kim
mesh_terms: D000143:Acids / Q000737:chemistry; D000327:Adsorption; D019149:Bioreactors; D020148:Butyric Acid / Q000737:chemistry*; D002244:Carbon / Q000737:chemistry; D046969:Clostridium acetobutylicum; D004798:Enzymes / Q000737:chemistry; D000431:Ethanol / Q000737:chemistry; D005285:Fermentation; D005662:Furaldehyde / Q000031:analogs & derivatives; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D007218:Industrial Microbiology / Q000379:methods*; D012624:Seaweed / Q000378:metabolism*; D013696:Temperature
chemical_list: D000143:Acids; D004798:Enzymes; D020148:Butyric Acid; D000431:Ethanol; C008046:5-hydroxymethylfurfural; D002244:Carbon; D005662:Furaldehyde
keywords: 5-Hydroxymethylfurfural; Butyric acid; Clostridium acetobutylicum KCTC 1790; Gelidium amansii