title: Nano-exploration of organic conditioning film formed on polymeric surfaces exposed to drinking water.
abstract: Adsorption of organic macromolecules onto surfaces in contact with waters forms a so-called conditioning film and induces modifications of the surface properties. Here, we characterized conditioning films formed onto two hydrophobic materials (used as pipe liner) and immersed for 24 h in tap water. Using combination of atomic force microscopy (AFM), and chemical force microscopy (CFM), we detected some changes in roughness and hydrophilic/hydrophobic balance of the surface of the tested coupons, and also the deposition of numerous organic polymers (few millions/cm2) randomly distributed on the surface. The maximum molecular extension of these organic polymers was in the range of 250-1250 nm according to the tested materials. Systematic analysis of the force curves with the theoretical models (WLC and FJC) allowed determining the proportion of rupture events related to the unfolding of both polysaccharide and polypeptide segments, which represented 75-80% and 20-25% of the analyzed curves, respectively. The number of autochthonous drinking water bacteria, which attached to the material within the same period of time was 10000-folds lower than the detected number of polymers attached to the surface. Even in drinking water systems with relatively low organic matter (dissolved organic carbon < 1.1 mg/L), the potential of formation of a conditioning biofilm is important.
authors.affiliation: CNRS - Université de Lorraine, Laboratoire de Chimie Physique et Microbiologie pour l'Environnement, LCPME, UMR 7564, Villers-lès-Nancy, F-54600, France. Electronic address: gregory.francius@univ-lorraine.fr.
authors.forename: Grégory
authors.initials: G
authors.lastname: Francius
authors.affiliation: CNRS - Université de Lorraine, Laboratoire de Chimie Physique et Microbiologie pour l'Environnement, LCPME, UMR 7564, Villers-lès-Nancy, F-54600, France.
authors.forename: Racha
authors.initials: R
authors.lastname: El Zein
authors.affiliation: EPHE, PSL Research University, Laboratoire de Chimie Physique et Microbiologie pour l'Environnement, LCPME, UMR 7564, Vandoeuvre-lès-Nancy, F-54500, France.
authors.forename: Laurence
authors.initials: L
authors.lastname: Mathieu
authors.affiliation: CNRS - Université de Lorraine, Laboratoire de Chimie Physique et Microbiologie pour l'Environnement, LCPME, UMR 7564, Villers-lès-Nancy, F-54600, France.
authors.forename: Florence
authors.initials: F
authors.lastname: Gosselin
authors.affiliation: Université de Lorraine - CNRS, Laboratoire interdisciplinaire des environnements continentaux, LIEC, UMR 7360, Metz, F-57070, France.
authors.forename: Armand
authors.initials: A
authors.lastname: Maul
authors.affiliation: CNRS - Université de Lorraine, Laboratoire de Chimie Physique et Microbiologie pour l'Environnement, LCPME, UMR 7564, Villers-lès-Nancy, F-54600, France.
authors.forename: Jean-Claude
authors.initials: JC
authors.lastname: Block
mesh_terms: D018441:Biofilms; D060766:Drinking Water; D057927:Hydrophobic and Hydrophilic Interactions; D018625:Microscopy, Atomic Force; D013499:Surface Properties
chemical_list: D060766:Drinking Water
keywords: AFM; Bacterial adhesion; Conditioning film; Drinking water; Hydrophobicity; Polymeric materials