title: [Effect of rhynchophylline on behaviors of methamphetamine-dependent zebrafish and the mechanism].
abstract: OBJECTIVE
  To observe the effect of rhynchophylline on methamphetamine-dependent zebrafish and explore the possible mechanism.
  
  
  METHODS
  Zebrafish were divided into control group, amphetamine group, low- (50 mg/kg) and high (100 mg/kg)-dose rhynchophylline groups, and ketamine (150 mg/kg) group. Conditioned place preference (CPP) was induced in zebrafish with methamphetamine, and the staying time in the drug box and the tracking map of the zebrafish were observed with Noldus Ethovision XT system. The protein expressions of TH, NR2B and GLUR2 in the brain of zebrafish with CPP were detected with Western blotting.
  
  
  RESULTS
  Compared with the control group, zebrafish in methamphetamine group showed significant variations in the staying time and swimming distance in the drug box after conditioning (P<0.05) with obvious alterations of NR2B, TH and GLUR2 expressions in the brain (P<0.05). Treatment of methamphetamine-dependent zebrafish with high-dose rhynchophylline significantly reduced the variations in the staying time and swimming distance in the drug box (P<0.05) and in the expressions of NR2B, TH and GLUR2 in the brain (P<0.05).
  
  
  CONCLUSIONS
  Rhynchophylline can inhibit methamphetamine dependence in zebrafish, the mechanism of which may involve the expressions of TH, NR2B and GLUR2 proteins in the brain.
authors.affiliation: School of Traditional Chinese Medicine, Southern Medical University, Guangzhou 510515, China.E-mail: 93043841@qq.com.
authors.forename: Yi-Fei
authors.initials: YF
authors.lastname: Chen
authors.forename: Ju
authors.initials: J
authors.lastname: Peng
authors.forename: Miao
authors.initials: M
authors.lastname: Fang
authors.forename: Yi
authors.initials: Y
authors.lastname: Liu
authors.forename: Ling-Hui
authors.initials: LH
authors.lastname: Nie
authors.forename: Zhi-Xian
authors.initials: ZX
authors.lastname: Mo
authors.forename: Ling-Ling
authors.initials: LL
authors.lastname: Zhu
mesh_terms: D000818:Animals; D001522:Behavior, Animal / Q000187:drug effects*; D001921:Brain / Q000187:drug effects*; D026121:Indole Alkaloids / Q000494:pharmacology*; D007649:Ketamine / Q000494:pharmacology; D008694:Methamphetamine / Q000494:pharmacology*; D000078183:Oxindoles; D015027:Zebrafish / Q000502:physiology*; D029961:Zebrafish Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D026121:Indole Alkaloids; D000078183:Oxindoles; D029961:Zebrafish Proteins; D008694:Methamphetamine; C052714:rhyncophylline; D007649:Ketamine