title: Prophylactic Effect of Lactobacillus pentosus strain S-PT84 on Candida Infection and Gastric Inflammation in a Murine Gastrointestinal Candidiasis Model.
abstract: We previously showed a prophylactic effect of Lactobacillus pentosus strain S-PT84 against oral candidiasis in mice. In the present study, we evaluated the protective effect of S-PT84 against Candida infection of the gastrointestinal tract. As the first step, we used an in vitro assay to compare the inhibitory effects of several lactobacilli (S-PT84 and Lactobacillus pentosus type strain JCM1558T, Lactobacillus gasseri type strain JCM1131Tand Lactobacillus casei type strain JCM1134T) on mycelial growth of Candida albicans. S-PT84 directly adhered to Candida cells and showed the strongest growth-inhibitory activity among the tested Lactobacillus strains. In the second experiment, we used an in vivo assay to evaluate the effect of S-PT84 ingestion on severity score of stomach lesion and gastric inflammation in a mouse model of gastrointestinal candidiasis. The severity scores were significantly improved by oral administration of S-PT84 (6 mg/ 200 μL), consistent with decreased coverage of stomach lesions by patchy whitish plaques. The attenuation of stomach lesion severity by S-PT84 was more pronounced than that obtained with L. gasseri type strain JCM1131T, consistent with the results of the above in vitro study. Histological analysis also indicated that S-PT84 prevented the adhesion of C. albicans to the stomach surface and suppressed stomach inflammation caused by neutrophil infiltration. Furthermore, S-PT84 also suppressed the vascular permeability observed in Candida-infected stomach. These results suggest that oral administration of S-PT84 might be effective not only in inhibiting Candida infection but also in preventing gastric inflammation induced by Candida infection.
authors.affiliation: Institute for Health Care Science, Suntory Wellness Limited.
authors.forename: Toshihiro
authors.initials: T
authors.lastname: Maekawa
authors.forename: A Sanae
authors.initials: AS
authors.lastname: Ishijima
authors.forename: Masayuki
authors.initials: M
authors.lastname: Ida
authors.forename: Takayuki
authors.initials: T
authors.lastname: Izumo
authors.forename: Yoshiko
authors.initials: Y
authors.lastname: Ono
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Shibata
authors.forename: Shigeru
authors.initials: S
authors.lastname: Abe
mesh_terms: D000284:Administration, Oral; D000818:Animals; D000898:Antibiosis; D002176:Candida albicans / Q000254:growth & development*; D002177:Candidiasis / Q000517:prevention & control*; D002199:Capillary Permeability; D004195:Disease Models, Animal; D005260:Female; D005767:Gastrointestinal Diseases / Q000517:prevention & control*; D007249:Inflammation / Q000517:prevention & control*; D000070018:Lactiplantibacillus pentosus; D008813:Mice, Inbred ICR; D020556:Neutrophil Infiltration; D019936:Probiotics / Q000008:administration & dosage*; D012720:Severity of Illness Index; D013270:Stomach / Q000382:microbiology