title: Metabolic response induced by parasitic plant-fungus interactions hinder amino sugar and nucleotide sugar metabolism in the host.
abstract: Infestation by the biotrophic pathogen Gymnosporangium asiaticum can be devastating for plant of the family Rosaceae. However, the phytopathology of this process has not been thoroughly elucidated. Using a metabolomics approach, we discovered the intrinsic activities that induce disease symptoms after fungal invasion in terms of microbe-induced metabolic responses. Through metabolic pathway enrichment and mapping, we found that the host altered its metabolite levels, resulting in accumulation of tetrose and pentose sugar alcohols, in response to this fungus. We then used a multiple linear regression model to evaluate the effect of the interaction between this abnormal accumulation of sugar alcohol and the group variable (control/parasitism). The results revealed that this accumulation resulted in deficiency in the supply of specific sugars, which led to a lack of amino sugar and nucleotide sugar metabolism. Halting this metabolism could hamper pivotal functions in the plant host, including cell wall synthesis and lesion repair. In conclusion, our findings indicate that altered metabolic responses that occur during fungal parasitism can cause deficiency in substrates in pivotal pathways and thereby trigger pathological symptoms.
authors.affiliation: College of Pharmacy, Seoul National University, Seoul 08826, Korea.
authors.forename: Dong-Kyu
authors.initials: DK
authors.lastname: Lee
authors.affiliation: Department of Statistics, Seoul National University, Seoul 08826, Korea.
authors.forename: Soohyun
authors.initials: S
authors.lastname: Ahn
authors.affiliation: College of Pharmacy, Seoul National University, Seoul 08826, Korea.
authors.forename: Hae Yoon
authors.initials: HY
authors.lastname: Cho
authors.affiliation: Department of Agricultural Biotechnology, College of Agriculture and Life Sciences, Seoul National University, Seoul 08826, Korea.
authors.forename: Hye Young
authors.initials: HY
authors.lastname: Yun
authors.affiliation: College of Pharmacy, Seoul National University, Seoul 08826, Korea.|Research Institute of Pharmaceutical Sciences, Seoul National University, Seoul 08826, Korea.|Faculty of Pharmacy, Ton Duc Thang University, Ho Chi Minh City, Vietnam.
authors.forename: Jeong Hill
authors.initials: JH
authors.lastname: Park
authors.affiliation: Department of Statistics, Seoul National University, Seoul 08826, Korea.
authors.forename: Johan
authors.initials: J
authors.lastname: Lim
authors.affiliation: School of Pharmacy, Sungkyunkwan University, Suwon 16419, Korea.
authors.forename: Jeongmi
authors.initials: J
authors.lastname: Lee
authors.affiliation: College of Pharmacy, Seoul National University, Seoul 08826, Korea.|Research Institute of Pharmaceutical Sciences, Seoul National University, Seoul 08826, Korea.|Plant Genomics and Breeding Institute, Seoul National University, Seoul 08826, Korea.
authors.forename: Sung Won
authors.initials: SW
authors.lastname: Kwon
mesh_terms: D000606:Amino Sugars / Q000172:deficiency*; D001487:Basidiomycota / Q000472:pathogenicity* / Q000502:physiology; D002851:Chromatography, High Pressure Liquid; D005638:Fruit / Q000378:metabolism / Q000382:microbiology; D008401:Gas Chromatography-Mass Spectrometry; D054884:Host-Pathogen Interactions; D053858:Metabolic Networks and Pathways / Q000502:physiology; D055442:Metabolome; D009711:Nucleotides / Q000172:deficiency*; D010935:Plant Diseases / Q000382:microbiology; D018515:Plant Leaves / Q000378:metabolism / Q000382:microbiology; D027824:Rosaceae / Q000378:metabolism* / Q000382:microbiology; D013402:Sugar Alcohols / Q000378:metabolism
chemical_list: D000606:Amino Sugars; D009711:Nucleotides; D013402:Sugar Alcohols