title: Anti bacterial Effectiveness of Electro- Chemically Activated (ECA) Water as a Root Canal Irrigant- An In-vitro Comparative Study.
abstract: BACKGROUND
  Sodium hypochlorite, is the irrigant of choice for many clinicians, but its strong toxic and damaging effects on vital periapical tissues is always a matter of concern. So, the search for a root canal irrigant with a broad antimicrobial spectrum yet with a limited toxicity on vital tissues is always desirable.
  
  
  OBJECTIVE
  The study evaluated antimicrobial efficacy of Electro-Chemically Activated (ECA) water as a root canal irrigant againstE. faecalis.
  
  
  METHODS
  Forty eight single rooted human teeth were decoronated to a length of 15mm. All teeth were divided into four test groups (group A - ECA anolyte, group B - 1% sodium hypochlorite, group C - 3% sodium hypochlorite, group D - distilled water) of 12 each. Only 1ml of Ringer's solution and calibrated suspensio ofE. faecaliswas injected into each canal, aspirated and placed on agar plates and incubated aerobically at 37°C for two days. The suspension was aspirated and spread onto the blood agar plate and incubated. All samples were irrigated with four test solutions and Brain Heart Infusion (BHI) solution was injected into each canal then aspirated and spread onto blood agar and incubated. After inoculations Colony Forming Unit (CFU) and optical density was measured under a microscope and spectrophotometer. The data obtained were statistically analysed by one way ANOVA and Dunkan's multiple range test.
  
  
  RESULTS
  CFU reduction was not statistically significant between the test groups. The optical density showed statistically significant difference between the test groups (p≤0.001).
  
  
  CONCLUSIONS
  The antimicrobial efficacy of ECA was found to be comparable to sodium hypochlorite solutions.
authors.affiliation: Reader, Department of Conservative Dentistry and Endontics, Kalinga Instute of Dental Sciences, KIIT University , Bhubaneswar, Odisha, India .
authors.forename: S
authors.initials: S
authors.lastname: Lata
authors.affiliation: Reader, Department of Conservative Dentistry and Endontics, Institute of Dental Sciences, SOA University , Bhubaneswar, Odisha, India .
authors.forename: Soumya Kanta
authors.initials: SK
authors.lastname: Mohanty
authors.affiliation: Reader, Department of Conservative Dentistry and Endontics, Kalinga Instute of Dental Sciences, KIIT University , Bhubaneswar, Odisha, India .
authors.forename: Prasanti Kumari
authors.initials: PK
authors.lastname: Pradhan
authors.affiliation: Reader, Department of Conservative Dentistry and Endontics, Kalinga Instute of Dental Sciences, KIIT University , Bhubaneswar, Odisha, India .
authors.forename: Gaurav
authors.initials: G
authors.lastname: Patri
authors.affiliation: Professor, Department of Conservative Dentistry and Endontics, Kalinga Instute of Dental Sciences, KIIT University , Bhubaneswar, Odisha, India .
authors.forename: Sachidananda Prasad
authors.initials: SP
authors.lastname: Sinha
authors.affiliation: Lecturer, Department of Conservative Dentistry and Endontics, Kalinga Instute of Dental Sciences, KIIT University , Bhubaneswar, Odisha, India .
authors.forename: Pratik
authors.initials: P
authors.lastname: Agrawal
keywords: Anolyte; E. faecalis; Oxidative potential water