title: Antioxidant potentiality of three herbal teas consumed in Bandundu rural areas of Congo.
abstract: The aim of this study was to evaluate and compare the cellular antioxidant activities of Lantana montevidensis, Lippia multiflora, and Ocimum gratissimum leaves often consumed as herbal teas in a rural area of Bandundu severely affected by konzo, which is related to oxidative damage. Consequently, dietary supplements with proven antioxidant potentialities could be of real interest to promote in this area. Phytochemical screening by TLC and HPLC-DAD of extracts revealed the presence of verbascoside as a major phenolic compound. Verbascoside in L. montevidensis and O. gratissimum is reported here for the first time. All extracts displayed high ABTS and DPPH radical-scavenging activities at the concentration range of 1-40 μg mL-1according to order: L. multiflora > O. gratissimum > L. montevidensis. L. multiflora showed the best cellular antioxidant activity using DCFH-DA on HL-60 monocytes assay at 1-20 μg mL-1. These herbal teas may be used as nutraceuticals for their potent antioxidant activity.
authors.affiliation: a Laboratory of Pharmacognosy, Center for Interdisciplinary Research on Medicines (CIRM) , University of Liège , Liège , Belgium.|b Faculty of Pharmaceutical Sciences, Centre d'Etudes des Substances Naturelles d'Origine Végétale (CESNOV) , University of Kinshasa , Kinshasa , Democratic Republic of Congo.
authors.forename: Paulin Mutwale
authors.initials: PM
authors.lastname: Kapepula
authors.affiliation: b Faculty of Pharmaceutical Sciences, Centre d'Etudes des Substances Naturelles d'Origine Végétale (CESNOV) , University of Kinshasa , Kinshasa , Democratic Republic of Congo.
authors.forename: Patricia
authors.initials: P
authors.lastname: Mbombo Mungitshi
authors.affiliation: c Center for Oxygen, Research, and Development (CORD), Institute of Chemistry B6a , University of Liège , Liège , Belgium.
authors.forename: Thierry
authors.initials: T
authors.lastname: Franck
authors.affiliation: c Center for Oxygen, Research, and Development (CORD), Institute of Chemistry B6a , University of Liège , Liège , Belgium.
authors.forename: Ange
authors.initials: A
authors.lastname: Mouithys-Mickalad
authors.affiliation: d Faculty of Medicine , University of Kinshasa , Kinshasa , Democratic Republic of Congo.|e Department of Parasitology , Institut National de Recherches Biomédicales (INRB) , Gombe , Democratic Republic of Congo.
authors.forename: Dieudonné
authors.initials: D
authors.lastname: Mumba Ngoyi
authors.affiliation: b Faculty of Pharmaceutical Sciences, Centre d'Etudes des Substances Naturelles d'Origine Végétale (CESNOV) , University of Kinshasa , Kinshasa , Democratic Republic of Congo.
authors.forename: Pascal Dibungi T
authors.initials: PDT
authors.lastname: Kalenda
authors.affiliation: b Faculty of Pharmaceutical Sciences, Centre d'Etudes des Substances Naturelles d'Origine Végétale (CESNOV) , University of Kinshasa , Kinshasa , Democratic Republic of Congo.
authors.forename: Nadege
authors.initials: N
authors.lastname: Kabamba Ngombe
authors.affiliation: c Center for Oxygen, Research, and Development (CORD), Institute of Chemistry B6a , University of Liège , Liège , Belgium.
authors.forename: Didier
authors.initials: D
authors.lastname: Serteyn
authors.affiliation: a Laboratory of Pharmacognosy, Center for Interdisciplinary Research on Medicines (CIRM) , University of Liège , Liège , Belgium.
authors.forename: Monique
authors.initials: M
authors.lastname: Tits
authors.affiliation: a Laboratory of Pharmacognosy, Center for Interdisciplinary Research on Medicines (CIRM) , University of Liège , Liège , Belgium.
authors.forename: Michel
authors.initials: M
authors.lastname: Frédérich
authors.affiliation: d Faculty of Medicine , University of Kinshasa , Kinshasa , Democratic Republic of Congo.|e Department of Parasitology , Institut National de Recherches Biomédicales (INRB) , Gombe , Democratic Republic of Congo.
authors.forename: Jean-Jacques
authors.initials: JJ
authors.lastname: Tamfum Muyembe
mesh_terms: D000975:Antioxidants / Q000032:analysis* / Q000494:pharmacology*; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D003223:Congo; D005452:Fluoresceins / Q000032:analysis; D016166:Free Radical Scavengers / Q000494:pharmacology; D005960:Glucosides / Q000032:analysis; D018922:HL-60 Cells; D006801:Humans; D020128:Inhibitory Concentration 50; D032410:Lantana / Q000737:chemistry; D032411:Lippia / Q000737:chemistry; D031339:Ocimum / Q000737:chemistry; D010636:Phenols / Q000032:analysis; D010936:Plant Extracts / Q000032:analysis / Q000737:chemistry / Q000494:pharmacology; D018515:Plant Leaves / Q000737:chemistry; D012424:Rural Population; D000069459:Teas, Herbal / Q000032:analysis; D059808:Polyphenols
chemical_list: D000975:Antioxidants; D005452:Fluoresceins; D016166:Free Radical Scavengers; D005960:Glucosides; D010636:Phenols; D010936:Plant Extracts; D000069459:Teas, Herbal; C029569:diacetyldichlorofluorescein; C058956:acteoside; D059808:Polyphenols
keywords: Congo D.R; Kahemba; Konzo; Lantana montevidensis; Lippia multiflora; Ocimum gratissimum; Oxidative stress