title: Long non-coding RNA SPRY4-IT1 promotes gallbladder carcinoma progression.
abstract: Gallbladder carcinoma (GBC) is the most common malignancy of the bile duct and patients with GBC have extremely poor prognoses. Long non-coding RNAs (lncRNAs) are found to be dysregulated in a variety of cancers, including GBC. SPRY4-IT1 has been recently revealed as oncogenic regulator in many cancers. However, whether SPRY4-IT1 is involved in GBC progression remains largely unknown. To investigate the role of SPRY4-IT1 in GBC, we evaluated the expression SPRY4-IT1 in GBC tissues and cell lines, and investigated the effect of SPRY4-IT1 knockdown on cell proliferation, migration and invasion of GBC in vitro. Our result showed that SPRY4-IT1 was upregulated in GBC tissues. Further experiments revealed that SPRY4-IT1 knockdown significantly inhibited GBC cell proliferation. Furthermore, inhibitory effects of SPRY4-IT1 on cell migration and invasion were partly associated with EMT process. In conclusion, these data suggest that SPRY4-IT1 could be an oncogene for GBC, and may be served as a candidate target for new therapies in human GBC.
authors.affiliation: Radiation Center, East Hepatobiliary Surgery Hospital, Second Military Medical University, Shanghai, 201805, China.
authors.forename: Liang
authors.initials: L
authors.lastname: Yang
authors.affiliation: Radiation Center, East Hepatobiliary Surgery Hospital, Second Military Medical University, Shanghai, 201805, China.
authors.forename: Xi
authors.initials: X
authors.lastname: Cheng
authors.affiliation: Mini-Invasive Intervention Center, Eastern Hepatobiliary Surgery Hospital, Second Military Medical University, Shanghai, 200438, China.
authors.forename: Naijian
authors.initials: N
authors.lastname: Ge
authors.affiliation: Hepatic Surgical Department VI, Eastern Hepatobiliary Surgery Hospital, The Second Military Medical University, Shanghai, 200438, China.
authors.forename: Weixing
authors.initials: W
authors.lastname: Guo
authors.affiliation: Department of Biliary Tract, Eastern Hepatobiliary Surgery Hospital, The Second Military Medical University, Shanghai 200438, China.
authors.forename: Feiling
authors.initials: F
authors.lastname: Feng
authors.affiliation: Radiation Center, East Hepatobiliary Surgery Hospital, Second Military Medical University, Shanghai, 201805, China.
authors.forename: Fuying
authors.initials: F
authors.lastname: Wan
mesh_terms: D014408:Biomarkers, Tumor; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D049109:Cell Proliferation / Q000235:genetics; D018450:Disease Progression; D058750:Epithelial-Mesenchymal Transition / Q000235:genetics; D005706:Gallbladder Neoplasms / Q000235:genetics* / Q000473:pathology*; D015972:Gene Expression Regulation, Neoplastic; D055785:Gene Knockdown Techniques; D006801:Humans; D062085:RNA, Long Noncoding / Q000235:genetics*
chemical_list: D014408:Biomarkers, Tumor; D062085:RNA, Long Noncoding; C000605823:long noncoding RNA SPRY4-IT1, human
keywords: EMT; GBC; SPRY4-IT1; lncRNA; metastasis