title: The visual system of harvestmen (Opiliones, Arachnida, Chelicerata) - a re-examination.
abstract: BACKGROUND
  The visual systems in chelicerates are poorly understood, even though they show strong variation in eye and visual neuropil architecture, thus may provide valuable insights for the understanding of chelicerate phylogeny and eye evolution. Comparable morphological characters are desperately sought for reconstructions of the phylogeny of Chelicerata, especially with respect to Arachnida. So far, reliable data exist only for Pycnogonida, Xiphosura, Scorpiones, and Araneae. The few earlier studies of the organisation of the visual system in harvestmen are contradictory concerning the number, morphology, and position of the visual neuropils.
  
  
  RESULTS
  We undertook a descriptive and comparative analysis of the neuroanatomy of the visual system in several phalangid harvestmen species. Various traditional and modern methods were used that allow comparisons with previous results (cobalt fills, DiI/DiO labelling, osmium ethyl gallate procedure, and TEM). The R-cells (photoreceptor and arhabdomeric cells) in the eyes of Opiliones are linked to a first and a second visual neuropil. The first visual neuropil receives input from all R-cell axons, in the second only few R-cells terminate in the distal part. Hence, the second visual neuropil is subdivided in a part with direct R-cell input and a part without. The arcuate body is located in a subsequent position with direct contact to the second visual neuropil.
  
  
  CONCLUSIONS
  This re-examination comes to conclusions different from those of all previous studies. The visual system of phalangid Opiliones occupies an intermediate position between Pycnogonida, Xiphosura, and Scorpiones on the one side, and Araneae on the other side. The projection of the R-cells is similar to that in the former grouping, the general neuropil arrangement to that in the latter taxon. However, more research on the visual systems in other chelicerate orders is needed in order to draw inferences on phylogeny or eye evolution.
authors.affiliation: Bavarian State Collection of Zoology, SNSB, Münchhausenstraße 21, 81247 Munich, Germany ; Department Biologie II, Ludwig-Maximilians-Universität München, Großhaderner Straße 2, 82152 Planegg-Martinsried, Germany.
authors.forename: Tobias
authors.identifier: 0000-0001-7115-3555
authors.initials: T
authors.lastname: Lehmann
authors.affiliation: Bavarian State Collection of Zoology, SNSB, Münchhausenstraße 21, 81247 Munich, Germany.
authors.forename: Eva
authors.initials: E
authors.lastname: Lodde-Bensch
authors.affiliation: Bavarian State Collection of Zoology, SNSB, Münchhausenstraße 21, 81247 Munich, Germany ; Department Biologie II, Ludwig-Maximilians-Universität München, Großhaderner Straße 2, 82152 Planegg-Martinsried, Germany ; GeoBioCenter, LMU, Richard -Wagner-Str. 10, 80333 Munich, Germany.
authors.forename: Roland R
authors.initials: RR
authors.lastname: Melzer
authors.affiliation: Bavarian State Collection of Zoology, SNSB, Münchhausenstraße 21, 81247 Munich, Germany.
authors.forename: Martina
authors.initials: M
authors.lastname: Metz
keywords: Arachnida; Central projections; Chelicerata; Opiliones; Phylogeny; Visual system