title: The combination of osthole with baicalin protects mice from Staphylococcus aureus pneumonia.
abstract: We reported the inhibition of α-Hemolysin (Hla) production in methicillin-resistant Staphylococcus aureus USA300 by osthole and further investigated the combination of osthole and baicalin in the treatment of staphylococcal pneumonia. Using cytotoxicity assays and a mouse model of intranasal lung infection, we evaluated the effect of combined therapy. Our results suggest that the combination of osthole and baicalin alleviated S. aureus-mediated A549 cell injury and protected mice from S. aureus pneumonia.
authors.affiliation: Key Laboratory of Zoonosis, Ministry of Education, Institute of Zoonosis, College of Veterinary Medicine, Jilin University, Changchun, China.
authors.forename: Shui
authors.initials: S
authors.lastname: Liu
authors.affiliation: Key Laboratory of Zoonosis, Ministry of Education, Institute of Zoonosis, College of Veterinary Medicine, Jilin University, Changchun, China.
authors.forename: Bowen
authors.initials: B
authors.lastname: Liu
authors.affiliation: Department of Biological Sciences, Purdue University, West Lafayette, IN, USA.
authors.forename: Zhao-Qing
authors.initials: ZQ
authors.lastname: Luo
authors.affiliation: Key Laboratory of Zoonosis, Ministry of Education, Institute of Zoonosis, College of Veterinary Medicine, Jilin University, Changchun, China.
authors.forename: Jiaming
authors.initials: J
authors.lastname: Qiu
authors.affiliation: Key Laboratory of Zoonosis, Ministry of Education, Institute of Zoonosis, College of Veterinary Medicine, Jilin University, Changchun, China.
authors.forename: Xuan
authors.initials: X
authors.lastname: Zhou
authors.affiliation: Key Laboratory of Zoonosis, Ministry of Education, Institute of Zoonosis, College of Veterinary Medicine, Jilin University, Changchun, China.
authors.forename: Gen
authors.initials: G
authors.lastname: Li
authors.affiliation: Key Laboratory of Zoonosis, Ministry of Education, Institute of Zoonosis, College of Veterinary Medicine, Jilin University, Changchun, China.
authors.forename: Bing
authors.initials: B
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Zoonosis, Ministry of Education, Institute of Zoonosis, College of Veterinary Medicine, Jilin University, Changchun, China.
authors.forename: Xuming
authors.initials: X
authors.lastname: Deng
authors.affiliation: Key Laboratory of Zoonosis, Ministry of Education, Institute of Zoonosis, College of Veterinary Medicine, Jilin University, Changchun, China. jldxyzg@sohu.com.
authors.forename: Zhenguo
authors.initials: Z
authors.lastname: Yang
authors.affiliation: Key Laboratory of Zoonosis, Ministry of Education, Institute of Zoonosis, College of Veterinary Medicine, Jilin University, Changchun, China. wjf927@jlu.edu.cn.
authors.forename: Jianfeng
authors.initials: J
authors.lastname: Wang
mesh_terms: D000072283:A549 Cells; D000818:Animals; D001427:Bacterial Toxins / Q000037:antagonists & inhibitors*; D003374:Coumarins / Q000008:administration & dosage* / Q000494:pharmacology; D004195:Disease Models, Animal; D004359:Drug Therapy, Combination; D005419:Flavonoids / Q000008:administration & dosage* / Q000494:pharmacology; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects; D006460:Hemolysin Proteins / Q000037:antagonists & inhibitors*; D006801:Humans; D055624:Methicillin-Resistant Staphylococcus aureus / Q000187:drug effects / Q000201:enzymology; D051379:Mice; D008826:Microbial Sensitivity Tests; D011023:Pneumonia, Staphylococcal / Q000517:prevention & control*
chemical_list: D001427:Bacterial Toxins; D003374:Coumarins; D005419:Flavonoids; D006460:Hemolysin Proteins; C021091:staphylococcal alpha-toxin; C038044:baicalin; C046627:osthol
keywords: Alpha-hemolysin; Baicalin; Osthole; Staphylococcus aureus