title: Multiple-locus variable-number tandem repeat fingerprinting as a method for rapid and cost-effective typing of animal-associated Staphylococcus aureus strains from lineages other than sequence type 398.
abstract: In veterinary medicine, Staphylococcus aureus is associated with a range of mild to severe infections. The high density of livestock in intensive farming systems increases the risk of disease spread and hampers its control and measures of prevention, making S. aureus one of the most important animal pathogens. Multiple-locus variable-number tandem repeat fingerprinting (MLVF) has been successfully applied to the characterization of livestock-associated meticillin-resistant Staphylococcus aureus (MRSA) ST398 but not to the characterization of a wide range of other animal isolates. The objective of the current study was to examine the effectiveness of MLVF for studying S. aureus strains isolated from households, farms and exotic animals in three regions of Poland. MLVF, random amplification of polymorphic DNA (RAPD), spa typing and diagnostic microarrays were compared to determine the most suitable combination of methods for veterinary purposes. MLVF generated results consistent with host and geographic origins, reflecting population structures with a high concordance to spa typing results. MLVF has been proven to be a rapid, highly discriminatory and cost-effective method suitable for molecular typing in veterinary settings.
authors.affiliation: Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Krakow, Poland.
authors.forename: Maja
authors.initials: M
authors.lastname: Kosecka-Strojek
authors.affiliation: Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Krakow, Poland.
authors.forename: Weronika M
authors.initials: WM
authors.lastname: Ilczyszyn
authors.affiliation: Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Krakow, Poland.
authors.forename: Aneta
authors.initials: A
authors.lastname: Buda
authors.affiliation: Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Krakow, Poland.
authors.forename: Klaudia
authors.initials: K
authors.lastname: Polakowska
authors.affiliation: Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Krakow, Poland.
authors.forename: Krzysztof
authors.initials: K
authors.lastname: Murzyn
authors.affiliation: Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Krakow, Poland.
authors.forename: Tomasz
authors.initials: T
authors.lastname: Panz
authors.affiliation: Center of Microbiological Research and Autovaccines, Krakow, Poland.
authors.forename: Anna
authors.initials: A
authors.lastname: Bialecka
authors.affiliation: Center of Microbiological Research and Autovaccines, Krakow, Poland.
authors.forename: Andrzej
authors.initials: A
authors.lastname: Kasprowicz
authors.affiliation: State College of Computer Sciences and Business Administration, Lomza, Poland.
authors.forename: Antoni
authors.initials: A
authors.lastname: Jakubczak
authors.affiliation: Faculty of Veterinary Medicine, Wroclaw University of Environmental and Life Sciences, Wroclaw, Poland.
authors.forename: Jaroslaw
authors.initials: J
authors.lastname: Krol
authors.affiliation: Faculty of Veterinary Medicine, Wroclaw University of Environmental and Life Sciences, Wroclaw, Poland.
authors.forename: Alina
authors.initials: A
authors.lastname: Wieliczko
authors.affiliation: Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Krakow, Poland.
authors.forename: Benedykt
authors.initials: B
authors.lastname: Wladyka
authors.affiliation: Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Krakow, Poland.
authors.forename: Jacek
authors.initials: J
authors.lastname: Miedzobrodzki
mesh_terms: D000818:Animals; D000829:Animals, Domestic / Q000382:microbiology*; D000068881:Animals, Exotic; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D015373:Bacterial Typing Techniques; D002415:Cats / Q000382:microbiology; D002417:Cattle / Q000382:microbiology; D002645:Chickens / Q000382:microbiology; D003362:Cost-Benefit Analysis; D016172:DNA Fingerprinting / Q000191:economics; D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics; D004285:Dogs / Q000382:microbiology; D018397:Equidae / Q000382:microbiology; D005191:Family Characteristics; D005838:Genotype; D058751:Livestock / Q000382:microbiology; D046228:Microarray Analysis; D008826:Microbial Sensitivity Tests; D018598:Minisatellite Repeats; D058889:Molecular Typing / Q000662:veterinary*; D002679:Pan troglodytes / Q000382:microbiology; D011044:Poland / Q000453:epidemiology; D011817:Rabbits / Q000382:microbiology; D013203:Staphylococcal Infections / Q000453:epidemiology / Q000382:microbiology / Q000662:veterinary*; D013211:Staphylococcus aureus / Q000145:classification* / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification
chemical_list: D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial