title: Identification of aberrantly expressed glycans in gastric cancer by integrated lectin microarray and mass spectrometric analyses.
abstract: Cancer progression is usually associated with alterations of glycan expression patterns. Little is known regarding global glycomics in gastric cancer, the most common type of epithelial cancer. We integrated lectin microarray and mass spectrometry (MS) methods to profile glycan expression in three gastric cancer cell lines (SGC-7901, HGC-27, and MGC-803) and one normal gastric epithelial cell line (GES-1). Significantly altered glycans were confirmed by lectin staining and MALDI-TOF/TOF-MS. The three cancer cell lines showed increased levels of core-fucosylated N-glycans, GalNAcα-Ser/Thr (Tn antigen), and Sia2-6Galβ1-4GlcNAc N-glycans, but reduced levels of biantennary N-glycans, Galβ1-3GalNAcα-Ser/Thr (T antigen), and (GlcNAc)n N-glycans. Lectin histochemistry was used to validate aberrant expression of four representative glycans (core-fucosylation, Sia2-6Galβ1-4GlcNAc, biantennary N-glycans, T antigen, recognized respectively by lectins LCA, SNA, PHA-E+L, and ACA) in clinical gastric cancer samples. Lower binding capacity for ACA was correlated with significantly poorer patient prognosis. Our findings indicate for the first time that glycans recognized by LCA, ACA, and PHA-E+L are aberrantly expressed in gastric cancer, and suggest that ACA is a potential prognostic factor for gastric cancer.
authors.affiliation: Wuxi Oncology Institute, Affiliated Hospital of Jiangnan University, Wuxi, Jiangsu, China.|College of Life Science, Northwest University, Xi'an, Shannxi, China.
authors.forename: Xiang
authors.initials: X
authors.lastname: Li
authors.affiliation: College of Life Science, Northwest University, Xi'an, Shannxi, China.|The Key Laboratory of Carbohydrate Chemistry & Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Guan
authors.affiliation: The Key Laboratory of Carbohydrate Chemistry & Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, China.|Bioimaging Core, Faculty of Health Sciences, University of Macau, Taipa, Macau SAR, China.
authors.forename: Dongliang
authors.initials: D
authors.lastname: Li
authors.affiliation: The Key Laboratory of Carbohydrate Chemistry & Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, China.
authors.forename: Zengqi
authors.initials: Z
authors.lastname: Tan
authors.affiliation: The Key Laboratory of Carbohydrate Chemistry & Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, China.
authors.forename: Ganglong
authors.initials: G
authors.lastname: Yang
authors.affiliation: The Key Laboratory of Carbohydrate Chemistry & Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, China.
authors.forename: Yanli
authors.initials: Y
authors.lastname: Wu
authors.affiliation: Wuxi Oncology Institute, Affiliated Hospital of Jiangnan University, Wuxi, Jiangsu, China.
authors.forename: Zhaohui
authors.initials: Z
authors.lastname: Huang
mesh_terms: D045744:Cell Line, Tumor; D006651:Histocytochemistry; D006801:Humans; D037102:Lectins / Q000378:metabolism*; D011134:Polysaccharides / Q000032:analysis* / Q000378:metabolism; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods; D013270:Stomach / Q000737:chemistry; D013274:Stomach Neoplasms / Q000737:chemistry*
chemical_list: D037102:Lectins; D011134:Polysaccharides
keywords: N-glycan; gastric cancer; lectin histochemistry; lectin microarray; mass spectrometry