title: Protein phosphatase type 2C PP2CA together with ABI1 inhibits SnRK2.4 activity and regulates plant responses to salinity.
abstract: Protein phosphatases 2C (PP2Cs) are important regulators of plant responses to abiotic stress. It is established that clade A PP2Cs inhibit ABA-activated SNF1-related protein kinases 2 (SnRK2s). Our recently published results show that ABI1, a member of clade A of PP2C is also a negative regulator of SnRK2.4, a kinase not activated in response to ABA. Here, we show that another member of this clade - PP2CA, interacts with and inhibits SnRK2.4. The salt-induced SnRK2.4/SnRK2.10 activity is higher in abi1-2 pp2ca-1 mutant than in wild type or single abi1 or pp2ca mutants, indicating that both phosphatases are inhibitors of SnRK2.4 and are at least partially redundant. Moreover, PP2CA together with ABI1 and SnRK2.4 regulates root growth in response to salinity.
authors.affiliation: a Institute of Biochemistry and Biophysics, Polish Academy of Sciences , Warsaw , Poland.
authors.forename: Ewa
authors.initials: E
authors.lastname: Krzywińska
authors.affiliation: a Institute of Biochemistry and Biophysics, Polish Academy of Sciences , Warsaw , Poland.
authors.forename: Anna
authors.initials: A
authors.lastname: Kulik
authors.affiliation: a Institute of Biochemistry and Biophysics, Polish Academy of Sciences , Warsaw , Poland.
authors.forename: Maria
authors.initials: M
authors.lastname: Bucholc
authors.affiliation: b Instituto de Biologia Molecular y Celular de Plantas, Consejo Superior de Investigaciones Científicas-Universidad Politécnica de Valencia , Valencia , Spain.
authors.forename: Maria A
authors.initials: MA
authors.lastname: Fernandez
authors.affiliation: b Instituto de Biologia Molecular y Celular de Plantas, Consejo Superior de Investigaciones Científicas-Universidad Politécnica de Valencia , Valencia , Spain.
authors.forename: Pedro L
authors.initials: PL
authors.lastname: Rodriguez
authors.affiliation: a Institute of Biochemistry and Biophysics, Polish Academy of Sciences , Warsaw , Poland.
authors.forename: Grażyna
authors.initials: G
authors.lastname: Dobrowolska
mesh_terms: D000040:Abscisic Acid / Q000494:pharmacology*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D018506:Gene Expression Regulation, Plant / Q000187:drug effects / Q000235:genetics; D009997:Osmotic Pressure / Q000502:physiology; D010749:Phosphoprotein Phosphatases / Q000235:genetics / Q000378:metabolism*; D011485:Protein Binding; D000071636:Protein Phosphatase 2C / Q000235:genetics / Q000378:metabolism*; D017346:Protein Serine-Threonine Kinases / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction / Q000235:genetics / Q000502:physiology; D012965:Sodium Chloride / Q000494:pharmacology
chemical_list: D029681:Arabidopsis Proteins; C492978:SnRK2 protein, Arabidopsis; D012965:Sodium Chloride; D000040:Abscisic Acid; C071570:SNF1-related protein kinases; D017346:Protein Serine-Threonine Kinases; C087434:ABI1 protein, Arabidopsis; C463465:AHG3 protein, Arabidopsis; D010749:Phosphoprotein Phosphatases; D000071636:Protein Phosphatase 2C
keywords: ABI1; AHG3; PP2C; PP2CA; SNF1-related protein kinases 2; SnRK2; SnRK2.4; osmotic stress signaling; phosphoprotein phosphatases; salinity