title: Hydrocarbon degraders establish at the costs of microbial richness, abundance and keystone taxa after crude oil contamination in permafrost environments.
abstract: Oil spills from pipeline ruptures are a major source of terrestrial petroleum pollution in cold regions. However, our knowledge of the bacterial response to crude oil contamination in cold regions remains to be further expanded, especially in terms of community shifts and potential development of hydrocarbon degraders. In this study we investigated changes of microbial diversity, population size and keystone taxa in permafrost soils at four different sites along the China-Russia crude oil pipeline prior to and after perturbation with crude oil. We found that crude oil caused a decrease of cell numbers together with a reduction of the species richness and shifts in the dominant phylotypes, while bacterial community diversity was highly site-specific after exposure to crude oil, reflecting different environmental conditions. Keystone taxa that strongly co-occurred were found to form networks based on trophic interactions, that is co-metabolism regarding degradation of hydrocarbons (in contaminated samples) or syntrophic carbon cycling (in uncontaminated samples). With this study we demonstrate that after severe crude oil contamination a rapid establishment of endemic hydrocarbon degrading communities takes place under favorable temperature conditions. Therefore, both endemism and trophic correlations of bacterial degraders need to be considered in order to develop effective cleanup strategies.
authors.affiliation: State Key Laboratory of Frozen Soils Engineering (SKLFSE), Northwest Institute of Eco-Environment and Resources, Chinese Academy of Sciences (CAS), Lanzhou, 730000, China.|GFZ German Research Centre for Geosciences, Helmholtz Centre Potsdam, Section 5.3 Geomicrobiology, Telegrafenberg, 14473 Potsdam, Germany.
authors.forename: Sizhong
authors.initials: S
authors.lastname: Yang
authors.affiliation: GFZ German Research Centre for Geosciences, Helmholtz Centre Potsdam, Section 5.3 Geomicrobiology, Telegrafenberg, 14473 Potsdam, Germany.|College of Electrical Engineering, Northwest University for Nationalities, Lanzhou, 730030, China.
authors.forename: Xi
authors.initials: X
authors.lastname: Wen
authors.affiliation: State Key Laboratory of Frozen Soils Engineering (SKLFSE), Northwest Institute of Eco-Environment and Resources, Chinese Academy of Sciences (CAS), Lanzhou, 730000, China.
authors.forename: Yulan
authors.initials: Y
authors.lastname: Shi
authors.affiliation: GFZ German Research Centre for Geosciences, Helmholtz Centre Potsdam, Section 5.3 Geomicrobiology, Telegrafenberg, 14473 Potsdam, Germany.
authors.forename: Susanne
authors.initials: S
authors.lastname: Liebner
authors.affiliation: State Key Laboratory of Frozen Soils Engineering (SKLFSE), Northwest Institute of Eco-Environment and Resources, Chinese Academy of Sciences (CAS), Lanzhou, 730000, China.
authors.forename: Huijun
authors.initials: H
authors.lastname: Jin
authors.affiliation: GFZ German Research Centre for Geosciences, Helmholtz Centre Potsdam, Section 5.3 Geomicrobiology, Telegrafenberg, 14473 Potsdam, Germany.
authors.forename: Amedea
authors.initials: A
authors.lastname: Perfumo
mesh_terms: D061271:Acidobacteria / Q000145:classification / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D039903:Actinobacteria / Q000145:classification / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D041963:Bacteroidetes / Q000145:classification / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D001673:Biodegradation, Environmental; D015169:Colony Count, Microbial; D004269:DNA, Bacterial / Q000235:genetics*; D000068536:Bacillota / Q000145:classification / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D006838:Hydrocarbons / Q000378:metabolism; D059013:Microbial Consortia / Q000235:genetics; D065946:Permafrost / Q000382:microbiology*; D010578:Petroleum / Q000378:metabolism*; D059392:Petroleum Pollution / Q000032:analysis; D010802:Phylogeny; D020560:Proteobacteria / Q000145:classification / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D012988:Soil Microbiology
chemical_list: D004269:DNA, Bacterial; D006838:Hydrocarbons; D010578:Petroleum; D012336:RNA, Ribosomal, 16S