title: Therapeutic effects of traditional Chinese medicine Niubeixiaohe in mouse tuberculosis models.
abstract: BACKGROUND
  The traditional Chinese medicine Niubeixiaohe (NBXH) is an effective anti-tuberculosis prescription, which is made up of Bulbus Fritillariae Cirrhosae, Rhizoma Bletillae, Radix Platycodonis, Fructus Arctii, Herba Houttuyniae and Glutinous rice. In this study, NBXH powder (I) and three kinds of NBXH extracts (II, III, and IV) were prepared. The water decoction of NBXH had been used to treat TB in clinic sixteen years suggested that it was effective to treat TB.
  
  
  OBJECTIVE
  This study evaluated the effects of different processing products of NBXH on mouse TB model in vivo and provide a new Chinese medicine for the clinical treatment of TB.
  
  
  METHODS
  In this study, 120 female BALB/c mice infected with Mycobacterium tuberculosis H37Rv, were treated with distilled water, M. vaccae vaccine, the low, middle and high doses of NBXH I, the low, middle and high doses of NBXH II, the low, middle and high doses of NBXH III, the low, middle and high doses of NBXH IV for 12 weeks, respectively.
  
  
  RESULTS
  The body weights of mice in all NBXH groups were higher than that in the water group. The weight indexes of the spleens in M. vaccae group, the middle dose of NBXH II group, the low dose of NBXH IV group and in the high dose of NBXH IV group were significantly lower than that in the water group(P<0.05). Compared with the water group, the spleen colony counts in the low dose of NBXH I group, the high dose of NBXH II group, the low dose of NBXH III group and the high dose of NBXH IV group reduced by 0.43, 0.46, 0.73, 0.58 logs (P<0.05), respectively. But the lung colony counts had no significant difference between each group. Pulmonary general pathology and histopathology displayed that the lung lesions in treatment groups were improved at certain degree, especially in the low dose of NBXH IIIand IV groups, in which their areas of the lesions were less than 50%, and the half normal lung structure in half of the mice could be observed.
  
  
  CONCLUSIONS
  Powder and three extracts of traditional Chinese medicine NBXH all had anti-tuberculosis therapeutic effects on mouse tuberculosis model, and this study provided a base for the further development of Chinese patent medicine NBXH. Also, this is the first report on comprehensive experimental research of NBXH extracts coming from six kinds of traditional Chinese medicine.
authors.affiliation: Army Tuberculosis Prevention and Control Key Laboratory, Beijing Key Laboratory of New Techniques of Tuberculosis Diagnosis and Treatment, Institute of Tuberculosis Research, The 309th Hospital of Chinese PLA, Beijing 100091, China. Electronic address: amy5919@sina.com.
authors.forename: Yan
authors.initials: Y
authors.lastname: Liang
authors.affiliation: Army Tuberculosis Prevention and Control Key Laboratory, Beijing Key Laboratory of New Techniques of Tuberculosis Diagnosis and Treatment, Institute of Tuberculosis Research, The 309th Hospital of Chinese PLA, Beijing 100091, China. Electronic address: wangxiaomei_1a@163.com.
authors.forename: Xiaomei
authors.initials: X
authors.lastname: Wang
authors.affiliation: Army Tuberculosis Prevention and Control Key Laboratory, Beijing Key Laboratory of New Techniques of Tuberculosis Diagnosis and Treatment, Institute of Tuberculosis Research, The 309th Hospital of Chinese PLA, Beijing 100091, China. Electronic address: xiangfeidexin_20@sina.com.
authors.forename: Jinying
authors.initials: J
authors.lastname: Song
authors.affiliation: Army Tuberculosis Prevention and Control Key Laboratory, Beijing Key Laboratory of New Techniques of Tuberculosis Diagnosis and Treatment, Institute of Tuberculosis Research, The 309th Hospital of Chinese PLA, Beijing 100091, China. Electronic address: wanglan922@163.com.
authors.forename: Lan
authors.initials: L
authors.lastname: Wang
authors.affiliation: Army Tuberculosis Prevention and Control Key Laboratory, Beijing Key Laboratory of New Techniques of Tuberculosis Diagnosis and Treatment, Institute of Tuberculosis Research, The 309th Hospital of Chinese PLA, Beijing 100091, China. Electronic address: lxrjchd@163.com.
authors.forename: Dan
authors.initials: D
authors.lastname: Chen
authors.affiliation: Army Tuberculosis Prevention and Control Key Laboratory, Beijing Key Laboratory of New Techniques of Tuberculosis Diagnosis and Treatment, Institute of Tuberculosis Research, The 309th Hospital of Chinese PLA, Beijing 100091, China. Electronic address: yangyourong@163.com.
authors.forename: Yourong
authors.initials: Y
authors.lastname: Yang
authors.affiliation: Army Tuberculosis Prevention and Control Key Laboratory, Beijing Key Laboratory of New Techniques of Tuberculosis Diagnosis and Treatment, Institute of Tuberculosis Research, The 309th Hospital of Chinese PLA, Beijing 100091, China. Electronic address: baixuejuan2003@163.com.
authors.forename: Xuejuan
authors.initials: X
authors.lastname: Bai
authors.affiliation: Army Tuberculosis Prevention and Control Key Laboratory, Beijing Key Laboratory of New Techniques of Tuberculosis Diagnosis and Treatment, Institute of Tuberculosis Research, The 309th Hospital of Chinese PLA, Beijing 100091, China. Electronic address: 2312262968@qq.com.
authors.forename: Jie
authors.initials: J
authors.lastname: Wang
authors.affiliation: Army Tuberculosis Prevention and Control Key Laboratory, Beijing Key Laboratory of New Techniques of Tuberculosis Diagnosis and Treatment, Institute of Tuberculosis Research, The 309th Hospital of Chinese PLA, Beijing 100091, China. Electronic address: Shiyingchang@sina.com.
authors.forename: Yingchang
authors.initials: Y
authors.lastname: Shi
authors.affiliation: Army Tuberculosis Prevention and Control Key Laboratory, Beijing Key Laboratory of New Techniques of Tuberculosis Diagnosis and Treatment, Institute of Tuberculosis Research, The 309th Hospital of Chinese PLA, Beijing 100091, China. Electronic address: chenshibing70@163.com.
authors.forename: Shibing
authors.initials: S
authors.lastname: Chen
authors.affiliation: Guangdong Qifang Pharmaceutical co., LTD, Guangzhou 510075, China. Electronic address: liujunalla@163.com.
authors.forename: Jun
authors.initials: J
authors.lastname: Liu
authors.affiliation: Guangdong Qifang Pharmaceutical co., LTD, Guangzhou 510075, China. Electronic address: qfy@21cn.net.
authors.forename: Cunwei
authors.initials: C
authors.lastname: Yang
authors.affiliation: Guangdong Qifang Pharmaceutical co., LTD, Guangzhou 510075, China. Electronic address: luohuafeng@outlook.com.
authors.forename: Huafeng
authors.initials: H
authors.lastname: Luo
authors.affiliation: Army Tuberculosis Prevention and Control Key Laboratory, Beijing Key Laboratory of New Techniques of Tuberculosis Diagnosis and Treatment, Institute of Tuberculosis Research, The 309th Hospital of Chinese PLA, Beijing 100091, China. Electronic address: liu.guangling@163.com.
authors.forename: Guangling
authors.initials: G
authors.lastname: Liu
authors.affiliation: Army Tuberculosis Prevention and Control Key Laboratory, Beijing Key Laboratory of New Techniques of Tuberculosis Diagnosis and Treatment, Institute of Tuberculosis Research, The 309th Hospital of Chinese PLA, Beijing 100091, China. Electronic address: wu-xueqiong@263.net.
authors.forename: Xueqiong
authors.initials: X
authors.lastname: Wu
mesh_terms: D000818:Animals; D000995:Antitubercular Agents / Q000494:pharmacology*; D058491:Bacterial Load; D001835:Body Weight / Q000187:drug effects; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D004339:Drug Compounding; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D005260:Female; D008168:Lung / Q000187:drug effects* / Q000382:microbiology / Q000473:pathology; D008807:Mice, Inbred BALB C; D009169:Mycobacterium tuberculosis / Q000187:drug effects* / Q000302:isolation & purification / Q000472:pathogenicity; D009929:Organ Size; D011208:Powders; D013154:Spleen / Q000187:drug effects / Q000382:microbiology / Q000473:pathology; D013997:Time Factors; D014397:Tuberculosis, Pulmonary / Q000188:drug therapy* / Q000382:microbiology / Q000473:pathology
chemical_list: D000995:Antitubercular Agents; D004365:Drugs, Chinese Herbal; D011208:Powders; C000620719:niubeixiaohe
keywords: Mycobacterium tuberculosis; Niubeixiaohe (NBXH); Traditional Chinese medicine; Tuberculosis