title: Increased CD4+CD45RA-FoxP3lowcells alter the balance between Treg and Th17 cells in colitis mice.
abstract: OBJECTIVE
  To investigate the role of regulatory T cell (Treg) subsets in the balance between Treg and T helper 17 (Th17) cells in various tissues from mice with dextran sulfate sodium-induced colitis.
  
  
  METHODS
  Treg cells, Treg cell subsets, Th17 cells, and CD4+CD25+FoxP3+IL-17+cells from the lamina propria of colon (LPC) and other ulcerative colitis (UC) mouse tissues were evaluated by flow cytometry. Forkhead box protein 3 (FoxP3), interleukin 17A (IL-17A), and RORC mRNA levels were assessed by real-time PCR, while interleukin-10 (IL-10) and IL-17A levels were detected with a Cytometric Beads Array.
  
  
  RESULTS
  In peripheral blood monocytes (PBMC), mesenteric lymph node (MLN), lamina propria of jejunum (LPJ) and LPC from UC mice, Treg cell numbers were increased (P< 0.05), and FoxP3 and IL-10 mRNA levels were decreased. Th17 cell numbers were also increased in PBMC and LPC, as were IL-17A levels in PBMC, LPJ, and serum. The number of FrI subset cells (CD4+CD45RA+FoxP3low) was increased in the spleen, MLN, LPJ, and LPC. FrII subset cells (CD4+CD45RA-FoxP3high) were decreased among PBMC, MLN, LPJ, and LPC, but the number of FrIII cells (CD4+CD45RA-FoxP3low) and CD4+CD25+FoxP3+IL-17A+cells was increased. FoxP3 mRNA levels in CD4+CD45RA-FoxP3lowcells decreased in PBMC, MLN, LPJ, and LPC in UC mice, while IL-17A and RORC mRNA increased. In UC mice the distribution of Treg, Th17 cells, CD4+CD45RA-FoxP3high, and CD4+CD45RA-FoxP3lowcells was higher in LPC relative to other tissues.
  
  
  CONCLUSIONS
  Increased numbers of CD4+CD45RA-FoxP3lowcells may cause an imbalance between Treg and Th17 cells that is mainly localized to the LPC rather than secondary lymphoid tissues.
authors.affiliation: Ya-Hui Ma, Jie Zhang, Xue Chen, Department of Gastroenterology, Beijing Anzhen Hospital, Capital Medical University, Beijing 100029, China.
authors.forename: Ya-Hui
authors.initials: YH
authors.lastname: Ma
authors.affiliation: Ya-Hui Ma, Jie Zhang, Xue Chen, Department of Gastroenterology, Beijing Anzhen Hospital, Capital Medical University, Beijing 100029, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Ya-Hui Ma, Jie Zhang, Xue Chen, Department of Gastroenterology, Beijing Anzhen Hospital, Capital Medical University, Beijing 100029, China.
authors.forename: Xue
authors.initials: X
authors.lastname: Chen
authors.affiliation: Ya-Hui Ma, Jie Zhang, Xue Chen, Department of Gastroenterology, Beijing Anzhen Hospital, Capital Medical University, Beijing 100029, China.
authors.forename: You-Fu
authors.initials: YF
authors.lastname: Xie
authors.affiliation: Ya-Hui Ma, Jie Zhang, Xue Chen, Department of Gastroenterology, Beijing Anzhen Hospital, Capital Medical University, Beijing 100029, China.
authors.forename: Yan-Hua
authors.initials: YH
authors.lastname: Pang
authors.affiliation: Ya-Hui Ma, Jie Zhang, Xue Chen, Department of Gastroenterology, Beijing Anzhen Hospital, Capital Medical University, Beijing 100029, China.
authors.forename: Xin-Juan
authors.initials: XJ
authors.lastname: Liu
mesh_terms: D000818:Animals; D018791:CD4 Lymphocyte Count; D003093:Colitis, Ulcerative / Q000139:chemically induced / Q000276:immunology* / Q000378:metabolism / Q000473:pathology; D003106:Colon / Q000276:immunology* / Q000378:metabolism / Q000473:pathology; D016264:Dextran Sulfate; D004195:Disease Models, Animal; D051858:Forkhead Transcription Factors / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D005786:Gene Expression Regulation; D020381:Interleukin-17 / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D017493:Leukocyte Common Antigens / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D008297:Male; D008810:Mice, Inbred C57BL; D057132:Nuclear Receptor Subfamily 1, Group F, Member 3 / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D010641:Phenotype; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D050378:T-Lymphocytes, Regulatory / Q000276:immunology* / Q000378:metabolism; D058504:Th17 Cells / Q000276:immunology* / Q000378:metabolism
chemical_list: D051858:Forkhead Transcription Factors; C498833:Foxp3 protein, mouse; C098680:Il17a protein, mouse; D020381:Interleukin-17; D057132:Nuclear Receptor Subfamily 1, Group F, Member 3; D012333:RNA, Messenger; C539901:Rorc protein, mouse; D016264:Dextran Sulfate; D017493:Leukocyte Common Antigens
keywords: Regulatory T cells; T helper 17 cells; Treg cells subsets; Ulcerative colitis