title: Methods for Isolation, Purification, and Propagation of Bacteriophages of Campylobacter jejuni.
abstract: Here, we describe the methods for isolation, purification, and propagation of Campylobacter jejuni bacteriophages from samples expected to contain high number of phages such as chicken feces. The overall steps are (1) liberation of phages from the sample material; (2) observation of plaque-forming units on C. jejuni lawns using a spot assay; (3) isolation of single plaques; (4) consecutive purification procedures; and (5) propagation of purified phages from a plate lysate to prepare master stocks.
authors.affiliation: Department of Veterinary Disease Biology, Faculty of Health and Medical Sciences, University of Copenhagen, Stigbøjlen 4, 1870, Frederiksberg C, Denmark. emre@sund.ku.dk.
authors.forename: Yilmaz Emre
authors.initials: YE
authors.lastname: Gencay
authors.affiliation: National Food Institute, Technical University of Denmark, Mørkhøj Bygade 19, 2860, Søborg, Denmark.
authors.forename: Tina
authors.initials: T
authors.lastname: Birk
authors.affiliation: Department of Veterinary Disease Biology, Faculty of Health and Medical Sciences, University of Copenhagen, Stigbøjlen 4, 1870, Frederiksberg C, Denmark.
authors.forename: Martine Camilla Holst
authors.initials: MC
authors.lastname: Sørensen
authors.affiliation: Department of Veterinary Disease Biology, Faculty of Health and Medical Sciences, University of Copenhagen, Stigbøjlen 4, 1870, Frederiksberg C, Denmark.
authors.forename: Lone
authors.initials: L
authors.lastname: Brøndsted
mesh_terms: D000362:Agar / Q000737:chemistry; D000818:Animals; D001435:Bacteriophages / Q000254:growth & development / Q000302:isolation & purification*; D002169:Campylobacter Infections / Q000382:microbiology / Q000662:veterinary*; D016123:Campylobacter jejuni / Q000254:growth & development / Q000302:isolation & purification / Q000821:virology*; D002645:Chickens / Q000382:microbiology*; D003470:Culture Media / Q000737:chemistry; D005243:Feces / Q000382:microbiology; D011201:Poultry Diseases / Q000382:microbiology*; D010948:Viral Plaque Assay
chemical_list: D003470:Culture Media; D000362:Agar
keywords: Bacteriophage; C. jejuni; Campylobacter; Isolation; Propagation; Purification; Titration