title: Photolysis of a peptide withN-peptidyl-7-nitroindoline units using two-photon absorption.
abstract: N-acyl-7-nitroindolines have been used as caged compounds to photorelease active molecules by a one- or two-photon excitation mechanism in biological systems. Here, we report the photolysis of a polypeptide that contains 7-nitroindoline units as linker moieties in its peptide backbone for potential materials engineering applications. Upon two-photon excitation with femtosecond laser light at 710 nm the photoreactive amide bond inN-peptidyl-7-nitroindolines is cleaved rendering short peptide fragments. Thus, this photochemical process changes the molecular composition at the laser focal volume. Gel modifications of this peptide can potentially be used for three-dimensional microstructure fabrication.
authors.affiliation: Department of Physics, The University of Texas at El Paso, 500 W University Avenue, El Paso, TX 79968, USA.
authors.forename: Kevin A
authors.initials: KA
authors.lastname: Hatch
authors.affiliation: Department of Chemistry, The University of Texas at El Paso, 500 W University Avenue, El Paso, TX 79968, USA.
authors.forename: Alfredo
authors.initials: A
authors.lastname: Ornelas
authors.affiliation: Department of Chemistry, The University of Texas at El Paso, 500 W University Avenue, El Paso, TX 79968, USA.
authors.forename: Kaitlyn N
authors.initials: KN
authors.lastname: Williams
authors.affiliation: Department of Metallurgical, Materials and Biomedical Engineering, The University of Texas at El Paso, 500 W University Avenue, El Paso, TX 79968, USA; Border Biomedical Research Center, The University of Texas at El Paso, 500 W University Avenue, El Paso, TX 79968, USA.
authors.forename: Thomas
authors.initials: T
authors.lastname: Boland
authors.affiliation: Department of Chemistry, The University of Texas at El Paso, 500 W University Avenue, El Paso, TX 79968, USA; Border Biomedical Research Center, The University of Texas at El Paso, 500 W University Avenue, El Paso, TX 79968, USA; kmichael@utep.edu.
authors.forename: Katja
authors.initials: K
authors.lastname: Michael
authors.affiliation: Department of Physics, The University of Texas at El Paso, 500 W University Avenue, El Paso, TX 79968, USA; Border Biomedical Research Center, The University of Texas at El Paso, 500 W University Avenue, El Paso, TX 79968, USA; cli@utep.edu.
authors.forename: Chunqiang
authors.initials: C
authors.lastname: Li
keywords: (160.1435) Biomaterials; (160.5335) Photosensitive materials; (190.4180) Multiphoton processes