title: Gastroprotective effect of esculin on ethanol-induced gastric lesion in mice.
abstract: The gastroprotective effect of esculin was investigated in a mouse model of ethanol-induced gastric lesion. Administration of esculin at doses of 5, 10, and 20 mg/kg body weight prior to ethanol ingestion led to significant gastroprotection compared with untreated mice. Gastric mucosal lesions were evaluated by macroscopic and histopathological alterations, lesion index, and myeloperoxidase (MPO) activity. Pretreatment with esculin significantly reduced macroscopic and histopathological damage, gastric lesion index, and MPO activity in a dose-dependent manner. Moreover, esculin significantly reduced nitric oxide (NO) production, inducible NO synthase (iNOS) levels, and nuclear factor-kappa B (NF-κB) p65 protein expression in gastric tissues after ethanol challenge. Analysis of inflammatory cytokines indicated that esculin pretreatment markedly suppressed the increased expression of tumor necrosis factor-alpha (TNF-α) and interleukin-6 (IL-6) in ethanol-treated mice. The results demonstrate a protective effect of esculin against gastric injury and suggest that the underlying mechanism might be associated with inhibition of NF-κB activation, which subsequently reduces expression of iNOS, TNF-α, and IL-6.
authors.affiliation: School of Pharmacy, Xi'an Jiaotong University, Xi'an, 710061, China.
authors.forename: Weifeng
authors.initials: W
authors.lastname: Li
authors.affiliation: School of Pharmacy, Xi'an Jiaotong University, Xi'an, 710061, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Wang
authors.affiliation: School of Pharmacy, Xi'an Jiaotong University, Xi'an, 710061, China.
authors.forename: Xiumei
authors.initials: X
authors.lastname: Wang
authors.affiliation: School of Pharmacy, Xi'an Jiaotong University, Xi'an, 710061, China.
authors.forename: Hailin
authors.initials: H
authors.lastname: Zhang
authors.affiliation: School of Pharmacy, Xi'an Jiaotong University, Xi'an, 710061, China.
authors.forename: Zehong
authors.initials: Z
authors.lastname: He
authors.affiliation: School of Pharmacy, Xi'an Jiaotong University, Xi'an, 710061, China.
authors.forename: Wenbing
authors.initials: W
authors.lastname: Zhi
authors.affiliation: School of Pharmacy, Xi'an Jiaotong University, Xi'an, 710061, China.
authors.forename: Fang
authors.initials: F
authors.lastname: Liu
authors.affiliation: School of Pharmacy, Xi'an Jiaotong University, Xi'an, 710061, China.
authors.forename: Xiaofeng
authors.initials: X
authors.lastname: Niu
mesh_terms: D000818:Animals; D016207:Cytokines / Q000378:metabolism; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D004929:Esculin / Q000008:administration & dosage / Q000494:pharmacology*; D000431:Ethanol / Q000633:toxicity*; D005753:Gastric Mucosa / Q000187:drug effects* / Q000473:pathology; D015850:Interleukin-6 / Q000378:metabolism; D008297:Male; D051379:Mice; D009569:Nitric Oxide / Q000378:metabolism; D009195:Peroxidase / Q000378:metabolism; D013276:Stomach Ulcer / Q000139:chemically induced / Q000517:prevention & control*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D016207:Cytokines; D015850:Interleukin-6; D014409:Tumor Necrosis Factor-alpha; D004929:Esculin; D009569:Nitric Oxide; D000431:Ethanol; D009195:Peroxidase
keywords: anti-inflammatory; esculin; gastric lesion; nuclear factor-κB