title: Circulating MicroRNAs as Novel Biomarkers of Stenosis Progression in Asymptomatic Carotid Stenosis.
abstract: Progression of asymptomatic carotid artery stenosis (ACAS) in patients with >50% luminal narrowing is considered a potential risk factor for ischemic stroke; however, subclinical molecular biomarkers of ACAS progression are lacking. Recent studies suggest a regulatory function for several microRNAs (miRNAs) on the evolution of carotid plaque, but its role in ACAS progression is mostly unknown. The aim of our study was to investigate a wide miRNA panel in peripheral blood exosomes from patients with ACAS to associate circulating miRNA expression profiles with stenosis progression.
  
  
  
  The study included 60 patients with ACAS carrying >50% luminal narrowing. First, miRNA expression profiles of circulating exosomes were determined by Affymetrix microarrays from plasma samples of 16 patients from the cohort. Second, those miRNAs among the most differentially expressed in patients with ACAS progression were quantified by real-time polymerase chain reaction in a separate replication cohort of 39 subjects within the patient sample.
  
  
  
  Our results showed that ACAS progression was associated with development of stroke. MiR-199b-3p, miR-27b-3p, miR-130a-3p, miR-221-3p, and miR-24-3p presented significant higher expression in those patients with ACAS progression.
  
  
  
  In conclusion, our study supports that specific circulating miRNA expression profiles could provide a new tool that complements the monitoring of ACAS progression, improving therapeutic approaches to prevent ischemic stroke.
authors.affiliation: From the IIS-HUIP La Fe, Valencia, Spain (S.D., D.S.); Thrombosis, Vascular Biology and Hemostasis Research Group, IIS-HUIP La Fe, Valencia, Spain (S.D., A.L.); Department of Neurology, HUIP La Fe, Valencia, Spain (D.G., J.I.T., G.F., V.P., A.L.); and Genomics Unit, HUIP La Fe, Valencia, Spain (D.S.). sandolzgi@gmail.com.
authors.forename: Sandra
authors.initials: S
authors.lastname: Dolz
authors.affiliation: From the IIS-HUIP La Fe, Valencia, Spain (S.D., D.S.); Thrombosis, Vascular Biology and Hemostasis Research Group, IIS-HUIP La Fe, Valencia, Spain (S.D., A.L.); Department of Neurology, HUIP La Fe, Valencia, Spain (D.G., J.I.T., G.F., V.P., A.L.); and Genomics Unit, HUIP La Fe, Valencia, Spain (D.S.).
authors.forename: David
authors.initials: D
authors.lastname: Górriz
authors.affiliation: From the IIS-HUIP La Fe, Valencia, Spain (S.D., D.S.); Thrombosis, Vascular Biology and Hemostasis Research Group, IIS-HUIP La Fe, Valencia, Spain (S.D., A.L.); Department of Neurology, HUIP La Fe, Valencia, Spain (D.G., J.I.T., G.F., V.P., A.L.); and Genomics Unit, HUIP La Fe, Valencia, Spain (D.S.).
authors.forename: José Ignacio
authors.initials: JI
authors.lastname: Tembl
authors.affiliation: From the IIS-HUIP La Fe, Valencia, Spain (S.D., D.S.); Thrombosis, Vascular Biology and Hemostasis Research Group, IIS-HUIP La Fe, Valencia, Spain (S.D., A.L.); Department of Neurology, HUIP La Fe, Valencia, Spain (D.G., J.I.T., G.F., V.P., A.L.); and Genomics Unit, HUIP La Fe, Valencia, Spain (D.S.).
authors.forename: Dolors
authors.initials: D
authors.lastname: Sánchez
authors.affiliation: From the IIS-HUIP La Fe, Valencia, Spain (S.D., D.S.); Thrombosis, Vascular Biology and Hemostasis Research Group, IIS-HUIP La Fe, Valencia, Spain (S.D., A.L.); Department of Neurology, HUIP La Fe, Valencia, Spain (D.G., J.I.T., G.F., V.P., A.L.); and Genomics Unit, HUIP La Fe, Valencia, Spain (D.S.).
authors.forename: Gerardo
authors.initials: G
authors.lastname: Fortea
authors.affiliation: From the IIS-HUIP La Fe, Valencia, Spain (S.D., D.S.); Thrombosis, Vascular Biology and Hemostasis Research Group, IIS-HUIP La Fe, Valencia, Spain (S.D., A.L.); Department of Neurology, HUIP La Fe, Valencia, Spain (D.G., J.I.T., G.F., V.P., A.L.); and Genomics Unit, HUIP La Fe, Valencia, Spain (D.S.).
authors.forename: Vera
authors.initials: V
authors.lastname: Parkhutik
authors.affiliation: From the IIS-HUIP La Fe, Valencia, Spain (S.D., D.S.); Thrombosis, Vascular Biology and Hemostasis Research Group, IIS-HUIP La Fe, Valencia, Spain (S.D., A.L.); Department of Neurology, HUIP La Fe, Valencia, Spain (D.G., J.I.T., G.F., V.P., A.L.); and Genomics Unit, HUIP La Fe, Valencia, Spain (D.S.).
authors.forename: Aida
authors.initials: A
authors.lastname: Lago
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D058070:Asymptomatic Diseases; D015415:Biomarkers / Q000097:blood; D016893:Carotid Stenosis / Q000097:blood* / Q000175:diagnosis*; D015331:Cohort Studies; D018450:Disease Progression; D005260:Female; D005500:Follow-Up Studies; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000097:blood*; D008875:Middle Aged
chemical_list: D015415:Biomarkers; D035683:MicroRNAs
keywords: biomarkers; blood cell; carotid stenosis; microRNAs; stroke