title: RNAi-mediated resistance to rice black-streaked dwarf virus in transgenic rice.
abstract: Rice black-streaked dwarf virus (RBSDV), a member of the genus Fijivirus in the family Reoviridae, causes significant economic losses in rice production in China and many other Asian countries. Development of resistant varieties by using conventional breeding methods is limited, as germplasm with high level of resistance to RBSDV have not yet been found. One of the most promising methods to confer resistance against RBSDV is the use of RNA interference (RNAi) technology. RBSDV non-structural protein P7-2, encoded by S7-2 gene, is a potential F-box protein and involved in the plant-virus interaction through the ubiquitination pathway. P8, encoded by S8 gene, is the minor core protein that possesses potent active transcriptional repression activity. In this study, we transformed rice calli using a mini-twin T-DNA vector harboring RNAi constructs of the RBSDV genes S7-2 or S8, and obtained plants harboring the target gene constructs and the selectable marker gene, hygromycin phosphotransferase (HPT). From the offspring of these transgenic plants, we obtained selectable marker (HPT gene)-free plants. Homozygous T5transgenic lines which harbored either S7-2-RNAi or S8-RNAi exhibited high level resistance against RBSDV under field infection pressure from indigenous viruliferous small brown planthoppers. Thus, our results showed that RNA interference with the expression of S7-2 or S8 genes seemed an effective way to induce high level resistance in rice against RBSD disease.
authors.affiliation: Key Laboratory of Plant Functional Genomics of the Ministry of Education, Jiangsu Key Laboratory of Crop Genetics and Physiology/Co-Innovation Center for Modern Production Technology of Grain Crops, Yangzhou University, Yangzhou, 225009, China.|Department of Crop Protection, Faculty of Agriculture, University of Khartoum, 13314, Khartoum North, Sudan.
authors.forename: Mohamed M S
authors.initials: MM
authors.lastname: Ahmed
authors.affiliation: Key Laboratory of Plant Functional Genomics of the Ministry of Education, Jiangsu Key Laboratory of Crop Genetics and Physiology/Co-Innovation Center for Modern Production Technology of Grain Crops, Yangzhou University, Yangzhou, 225009, China.
authors.forename: Shiquan
authors.initials: S
authors.lastname: Bian
authors.affiliation: Key Laboratory of Plant Functional Genomics of the Ministry of Education, Jiangsu Key Laboratory of Crop Genetics and Physiology/Co-Innovation Center for Modern Production Technology of Grain Crops, Yangzhou University, Yangzhou, 225009, China.
authors.forename: Muyue
authors.initials: M
authors.lastname: Wang
authors.affiliation: Key Laboratory of Plant Functional Genomics of the Ministry of Education, Jiangsu Key Laboratory of Crop Genetics and Physiology/Co-Innovation Center for Modern Production Technology of Grain Crops, Yangzhou University, Yangzhou, 225009, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Zhao
authors.affiliation: Key Laboratory of Plant Functional Genomics of the Ministry of Education, Jiangsu Key Laboratory of Crop Genetics and Physiology/Co-Innovation Center for Modern Production Technology of Grain Crops, Yangzhou University, Yangzhou, 225009, China.
authors.forename: Bingwei
authors.initials: B
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Plant Functional Genomics of the Ministry of Education, Jiangsu Key Laboratory of Crop Genetics and Physiology/Co-Innovation Center for Modern Production Technology of Grain Crops, Yangzhou University, Yangzhou, 225009, China.
authors.forename: Qiaoquan
authors.initials: Q
authors.lastname: Liu
authors.affiliation: Key Laboratory of Plant Functional Genomics of the Ministry of Education, Jiangsu Key Laboratory of Crop Genetics and Physiology/Co-Innovation Center for Modern Production Technology of Grain Crops, Yangzhou University, Yangzhou, 225009, China.
authors.forename: Changquan
authors.initials: C
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Plant Functional Genomics of the Ministry of Education, Jiangsu Key Laboratory of Crop Genetics and Physiology/Co-Innovation Center for Modern Production Technology of Grain Crops, Yangzhou University, Yangzhou, 225009, China.
authors.forename: Shuzhu
authors.initials: S
authors.lastname: Tang
authors.affiliation: Key Laboratory of Plant Functional Genomics of the Ministry of Education, Jiangsu Key Laboratory of Crop Genetics and Physiology/Co-Innovation Center for Modern Production Technology of Grain Crops, Yangzhou University, Yangzhou, 225009, China.
authors.forename: Minghong
authors.initials: M
authors.lastname: Gu
authors.affiliation: Key Laboratory of Plant Functional Genomics of the Ministry of Education, Jiangsu Key Laboratory of Crop Genetics and Physiology/Co-Innovation Center for Modern Production Technology of Grain Crops, Yangzhou University, Yangzhou, 225009, China. hxyu@yzu.edu.cn.
authors.forename: Hengxiu
authors.initials: H
authors.lastname: Yu
mesh_terms: D002681:China; D060467:Disease Resistance / Q000235:genetics*; D044783:F-Box Proteins / Q000235:genetics*; D012275:Oryza / Q000235:genetics* / Q000254:growth & development / Q000821:virology; D010935:Plant Diseases / Q000235:genetics* / Q000821:virology; D010942:Plant Viruses / Q000235:genetics / Q000472:pathogenicity; D030821:Plants, Genetically Modified / Q000235:genetics / Q000254:growth & development / Q000821:virology; D034622:RNA Interference; D012087:Reoviridae / Q000235:genetics / Q000472:pathogenicity
chemical_list: D044783:F-Box Proteins
keywords: RBSDV; RNA interference; Resistance; Selectable marker free (SMF); Transgenic rice