title: CD11d β2 integrin expression on human NK, B, and γδ T cells.
abstract: The CD11d integrin is expressed on the cell surface of leukocytes that belong to the myeloid lineage, but its expression on lymphocytes remains unexplored. To test the hypothesis that CD11d is expressed on lymphocyte subsets, we employed a multicolor flow cytometry panel to identify CD11d expression on B, NK, CD4+and CD8+αβ T cells (αβTc), and γδ T cells (γδTc) in human PBMC samples. CD11d was highly expressed on NK cells, B cells, and γδTc, but not αβTc. CD11d expression was higher on freshly isolated γδTc compared with αβTc from healthy donors, yet both inter- and intradonor variability was evident. Over time in primary culture, we consistently observed higher CD11d levels on γδTc compared with αβTc from the same donor. Furthermore, CD11d expression on γδTc increased over time and correlated with levels of IL-2 supplementation. Of interest, a greater percentage of Vδ1 γδTc expressed CD11d than did Vδ2 γδTc, which suggested differential roles for this integrin that may segregate with γδTc subsets. These results expand the potential for CD11d to regulate lymphocyte migration and tissue retention, and illuminate the possibility of a previously unconsidered role for CD11d in leukocyte biology and disease.
authors.affiliation: Molecular Medicine, Robarts Research Institute, The University of Western Ontario, London, Ontario, Canada.|Department of Oncology, University of Alberta, Edmonton, Alberta, Canada.
authors.forename: Gabrielle M
authors.initials: GM
authors.lastname: Siegers
authors.affiliation: Molecular Medicine, Robarts Research Institute, The University of Western Ontario, London, Ontario, Canada.
authors.forename: Christy R
authors.initials: CR
authors.lastname: Barreira
authors.affiliation: Department of Oncology, University of Alberta, Edmonton, Alberta, Canada.
authors.forename: Lynne-Marie
authors.initials: LM
authors.lastname: Postovit
authors.affiliation: Molecular Medicine, Robarts Research Institute, The University of Western Ontario, London, Ontario, Canada; dekaban@robarts.ca.|Department of Microbiology and Immunology, The University of Western Ontario, London, Ontario, Canada.
authors.forename: Gregory A
authors.initials: GA
authors.lastname: Dekaban
mesh_terms: D001402:B-Lymphocytes / Q000378:metabolism*; D018845:CD11 Antigens / Q000378:metabolism*; D002469:Cell Separation; D002478:Cells, Cultured; D006801:Humans; D039001:Integrin alpha Chains / Q000378:metabolism*; D007376:Interleukin-2 / Q000378:metabolism; D007694:Killer Cells, Natural / Q000378:metabolism*; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000378:metabolism; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000378:metabolism*; D014019:Tissue Donors
chemical_list: D018845:CD11 Antigens; C406812:ITGAD protein, human; D039001:Integrin alpha Chains; D007376:Interleukin-2; D016693:Receptors, Antigen, T-Cell, alpha-beta; D016692:Receptors, Antigen, T-Cell, gamma-delta
keywords: IL-2; acute spinal cord injury; gamma delta T cells; inflammation; innate immunity