title: Developmental bisphenol A exposure impairs sperm function and reproduction in zebrafish.
abstract: The developmental and reproductive toxicity of bisphenol A (BPA) has been demonstrated in a variety of model systems. Zebrafish (Danio rerio) were waterborne-exposed to BPA during three different developmental stages: embryonic period:6 h post fertilization (hpf) to 5 months post fertilization (mpf); larval period: 6 days post fertilization (dpf) to 5 mpf; and sexually mature period: 3 mpf to 5 mpf. Evaluations included F0 adult growth, reproduction parameters, and F1 offspring development. BPA exposure did not affect zebrafish growth in any of exposure groups. Testis weight was decreased only following the 6 hpf to 5 mpf 0.001 μM BPA exposure. The lowest effect level indicated by a reduction in sperm volume, density, motility, and velocity across a range of exposure durations was 0.001 μM, with all but sperm density significant for the longest exposure duration, which was also the only significant endpoint for the lowest exposure concentration in the 3-5 mpf exposure group. Nonmonotonic concentration-response curves were noted for all F0 reproductive endpoints for at least one of the two longest exposure durations. For the F1 offspring of fish exposed from 6 hpf to 5 mpf, malformations and mortality were increased following 0.001 μM BPA exposure, while egg production and fertilization were reduced in higher concentration treatment groups. Overall, BPA exposure during three different developmental periods impaired zebrafish reproductive development, with most significance changes found in the lowest concentration treatment groups. Genetic impacts on gamete development may underlie the secondary effects of reduced fertilization rate, embryonic mortality, and malformations.
authors.affiliation: Institute of Environmental Safety and Human Health, Wenzhou Medical University, Wenzhou 325035, China.
authors.forename: Jiangfei
authors.initials: J
authors.lastname: Chen
authors.affiliation: Department of Environmental and Molecular Toxicology, The Sinnhuber Aquatic Research Laboratory, and the Environmental Health Sciences Center, Oregon State University, Corvallis, OR 97333, USA.
authors.forename: Katerine S
authors.initials: KS
authors.lastname: Saili
authors.affiliation: Institute of Environmental Safety and Human Health, Wenzhou Medical University, Wenzhou 325035, China.
authors.forename: Yueqin
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Institute of Environmental Safety and Human Health, Wenzhou Medical University, Wenzhou 325035, China.
authors.forename: Lelin
authors.initials: L
authors.lastname: Li
authors.affiliation: Institute of Environmental Safety and Human Health, Wenzhou Medical University, Wenzhou 325035, China.
authors.forename: Yuxin
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: Institute of Environmental Safety and Human Health, Wenzhou Medical University, Wenzhou 325035, China.
authors.forename: Yinhang
authors.initials: Y
authors.lastname: Jia
authors.affiliation: Institute of Environmental Safety and Human Health, Wenzhou Medical University, Wenzhou 325035, China.
authors.forename: Chenglian
authors.initials: C
authors.lastname: Bai
authors.affiliation: Department of Environmental and Molecular Toxicology, The Sinnhuber Aquatic Research Laboratory, and the Environmental Health Sciences Center, Oregon State University, Corvallis, OR 97333, USA.
authors.forename: Robert L
authors.initials: RL
authors.lastname: Tanguay
authors.affiliation: Institute of Environmental Safety and Human Health, Wenzhou Medical University, Wenzhou 325035, China.
authors.forename: Qiaoxiang
authors.initials: Q
authors.lastname: Dong
authors.affiliation: Institute of Environmental Safety and Human Health, Wenzhou Medical University, Wenzhou 325035, China. Electronic address: cjhuang5711@163.com.
authors.forename: Changjiang
authors.initials: C
authors.lastname: Huang
mesh_terms: D000818:Animals; D001559:Benzhydryl Compounds / Q000633:toxicity*; D004305:Dose-Response Relationship, Drug; D005306:Fertilization / Q000187:drug effects / Q000502:physiology*; D016166:Free Radical Scavengers / Q000633:toxicity*; D008297:Male; D010636:Phenols / Q000633:toxicity*; D012098:Reproduction / Q000187:drug effects / Q000502:physiology*; D013094:Spermatozoa / Q000187:drug effects / Q000502:physiology*; D014874:Water Pollutants, Chemical / Q000633:toxicity*; D015027:Zebrafish / Q000254:growth & development*; D000098826:Bisphenol A Compounds
chemical_list: D001559:Benzhydryl Compounds; D016166:Free Radical Scavengers; D010636:Phenols; D014874:Water Pollutants, Chemical; C006780:bisphenol A; D000098826:Bisphenol A Compounds
keywords: Bisphenol A; Chronic exposure; Procreation ability; Reproduction; Sperm motility; Zebrafish