title: Amperometric Biosensors for Real Time Assays of Organophosphates.
abstract: An amperometric biosensor based on acetylcholinesterase (AChE) immobilized in gelatin was used to develop an assay for the organophosphate paraoxon. The more traditional manner employing preincubation was used for comparison between measurement procedures, although the aim of the study was to examine the performance of the biosensor for real time monitoring of organophosphates. The biosensor was immersed in a reaction chamber and paraoxon was injected inside. We were able to detect 200 pg of paraoxon within one minute or 2.5 ppb when the biosensor was preincubed in the sample solution for 15 minutes. The practical impact and expectations are discussed.
authors.affiliation: Center of Advanced Studies, Faculty of Military Health Sciences, University of Defence, Hradec Kralove, Czech Republic. rau@atlas.cz.|Department of Toxicology, Faculty of Military Health Sciences, University of Defence, Hradec Kralove, Czech Republic. rau@atlas.cz.|Center of Biological Defense, Techonin, Czech Republic. rau@atlas.cz.
authors.forename: Miroslav
authors.initials: M
authors.lastname: Pohanka
authors.affiliation: Center of Advanced Studies, Faculty of Military Health Sciences, University of Defence, Hradec Kralove, Czech Republic. jun@pmfhk.cz.|Department of Toxicology, Faculty of Military Health Sciences, University of Defence, Hradec Kralove, Czech Republic. jun@pmfhk.cz.
authors.forename: Daniel
authors.initials: D
authors.lastname: Jun
authors.affiliation: Center of Advanced Studies, Faculty of Military Health Sciences, University of Defence, Hradec Kralove, Czech Republic. kucakam@pmfhk.cz.|Department of Toxicology, Faculty of Military Health Sciences, University of Defence, Hradec Kralove, Czech Republic. kucakam@pmfhk.cz.
authors.forename: Kamil
authors.initials: K
authors.lastname: Kuca
keywords: Organophosphate; acetylcholinesterase; assay; biosensor; electrochemical; paraoxon