title: The plasma virome of febrile adult Kenyans shows frequent parvovirus B19 infections and a novel arbovirus (Kadipiro virus).
abstract: Viral nucleic acids present in the plasma of 498 Kenyan adults with unexplained fever were characterized by metagenomics analysis of 51 sample pools. The highest to lowest fraction of plasma pools was positive for parvovirus B19 (75 %), pegivirus C (GBV-C) (67 %), alpha anellovirus (59 %), gamma anellovirus (55 %), beta anellovirus (41 %), dengue virus genotype 2 (DENV-2) (16 %), human immunodeficiency virus type 1 (6 %), human herpesvirus 6 (6 %), HBV (4 %), rotavirus (4 %), hepatitis B virus (4 %), rhinovirus C (2 %), Merkel cell polyomavirus (MCPyV; 2 %) and Kadipiro virus (2 %). Ranking by overall percentage of viral reads yielded similar results. Characterization of viral nucleic acids in the plasma of a febrile East African population showed a high frequency of parvovirus B19 and DENV infections and detected a reovirus (Kadipiro virus) previously reported only in Asian Culex mosquitoes, providing a baseline to compare with future virome studies to detect emerging viruses in this region.
authors.affiliation: Centre for Geographic Medicine Research - Coast, Kenya Medical Research Institute, Kilifi, Kenya.|Blood Systems Research Institute, San Francisco, CA, USA.
authors.forename: Carolyne N
authors.initials: CN
authors.lastname: Ngoi
authors.affiliation: Blood Systems Research Institute, San Francisco, CA, USA.|Programa de Oncovirologia, Instituto Nacional de Câncer, Rio de Janeiro, Brazil.
authors.forename: Juliana
authors.initials: J
authors.lastname: Siqueira
authors.affiliation: Blood Systems Research Institute, San Francisco, CA, USA.
authors.forename: Linlin
authors.initials: L
authors.lastname: Li
authors.affiliation: Blood Systems Research Institute, San Francisco, CA, USA.
authors.forename: Xutao
authors.initials: X
authors.lastname: Deng
authors.affiliation: Centre for Geographic Medicine Research - Coast, Kenya Medical Research Institute, Kilifi, Kenya.
authors.forename: Peter
authors.initials: P
authors.lastname: Mugo
authors.affiliation: Centre for Geographic Medicine Research - Coast, Kenya Medical Research Institute, Kilifi, Kenya.|University of Washington, Seattle, WA, USA.
authors.forename: Susan M
authors.initials: SM
authors.lastname: Graham
authors.affiliation: International AIDS Vaccine Initiative, New York, NY, USA.|Department of Epidemiology and Biostatistics, University of California at San Francisco, CA, USA.
authors.forename: Matt A
authors.initials: MA
authors.lastname: Price
authors.affiliation: Centre for Geographic Medicine Research - Coast, Kenya Medical Research Institute, Kilifi, Kenya.|Nuffield Department of Medicine, University of Oxford, Headington, UK.
authors.forename: Eduard J
authors.initials: EJ
authors.lastname: Sanders
authors.affiliation: Department of Laboratory Medicine, University of California at San Francisco, CA, USA.|Blood Systems Research Institute, San Francisco, CA, USA.
authors.forename: Eric
authors.initials: E
authors.lastname: Delwart
mesh_terms: D000328:Adult; D018162:Coltivirus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D004279:DNA, Viral / Q000097:blood* / Q000235:genetics; D005260:Female; D005334:Fever / Q000097:blood / Q000821:virology*; D006801:Humans; D007630:Kenya; D008297:Male; D016732:Parvovirus B19, Human / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D010802:Phylogeny; D014777:Virus Diseases / Q000097:blood / Q000821:virology*; D014780:Viruses / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D055815:Young Adult
chemical_list: D004279:DNA, Viral