title: Injectable Polysaccharide Hydrogels as Biocompatible Platforms for Localized and Sustained Delivery of Antibiotics for Preventing Local Infections.
abstract: Biocompatible and antibacterial hydrogels have received increasing attention for preventing local bacterial infections. In this study, a type of polysaccharide hydrogels is prepared via the Schiff-based reaction at physiological conditions. The gelation time and mechanical property of the hydrogels are found to be dependent on the polysaccharide concentration and the polysaccharide weight ratio. 3-(4,5-Dimethyl-thiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay and live/dead assay indicate that the hydrogels display nontoxicity in vitro. After subcutaneous injection into rats, the hydrogels exhibit an acceptable biocompatibility in vivo. Furthermore, the bacterial inhibition tests by shaking flask method and agar disc-diffusion method demonstrate that the ceftriaxone-sodium-loaded hydrogels have remarkable antibacterial properties in vitro. The in vivo anti-infective tests further display that the antibiotic-loaded hydrogels display excellent anti-infective efficacies in both superficial and deep tissue infection. Consequently, the injectable and biocompatible polysaccharide hydrogels may serve as promising platforms for localized, sustained delivery of antibiotics for preventing local infections.
authors.affiliation: Key Laboratory of Polymer Ecomaterials, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, 130022, P. R. China.|School of Materials Science and Engineering, Sun Yat-Sen University, Guangzhou, 510275, P. R. China.
authors.forename: Ziyi
authors.initials: Z
authors.lastname: Li
authors.affiliation: Key Laboratory of Polymer Ecomaterials, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, 130022, P. R. China.
authors.forename: Chaoliang
authors.initials: C
authors.lastname: He
authors.affiliation: Department of Orthopaedics, The Second Hospital of Jilin University, Changchun, 130041, P. R. China.
authors.forename: Baoming
authors.initials: B
authors.lastname: Yuan
authors.affiliation: Department of Orthopaedics, The Second Hospital of Jilin University, Changchun, 130041, P. R. China.
authors.forename: Xiaoming
authors.initials: X
authors.lastname: Dong
authors.affiliation: Key Laboratory of Polymer Ecomaterials, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, 130022, P. R. China.
authors.forename: Xuesi
authors.initials: X
authors.lastname: Chen
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology / Q000627:therapeutic use*; D001672:Biocompatible Materials / Q000737:chemistry*; D002443:Ceftriaxone / Q000494:pharmacology; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D048271:Chitosan / Q000737:chemistry; D003692:Delayed-Action Preparations; D003911:Dextrans / Q000737:chemistry; D004926:Escherichia coli / Q000187:drug effects; D005612:Freeze Drying; D020100:Hydrogels / Q000737:chemistry*; D007267:Injections; D008297:Male; D008422:Materials Testing; D008807:Mice, Inbred BALB C; D008855:Microscopy, Electron, Scanning; D011134:Polysaccharides / Q000737:chemistry*; D013203:Staphylococcal Infections / Q000188:drug therapy* / Q000382:microbiology; D013211:Staphylococcus aureus / Q000187:drug effects
chemical_list: D000900:Anti-Bacterial Agents; D001672:Biocompatible Materials; D003692:Delayed-Action Preparations; D003911:Dextrans; D020100:Hydrogels; D011134:Polysaccharides; D002443:Ceftriaxone; D048271:Chitosan
keywords: anti-infective hydrogels; antibiotic delivery; bacterial inhibition; injectable hydrogels; polysaccharide hydrogels