title: Rapid identification of Robinsoniella peoriensis using specific 16S rRNA gene PCR primers.
abstract: Robinsoniella peoriensis is a Gram-positive, spore-forming anaerobic bacterium initially isolated and characterized from swine manure and feces. Since then strains of this species have been identified from a variety of mammalian and other GI tracts. More recently strains of this species have been isolated from a plethora of human infections. Therefore, it is of great interest to develop methods to rapidly identify this microorganism in the medical and other laboratories. This report describes the use of PCR primers targeting the 16S rRNA gene of R. peoriensis to identify strains of this bacterium.
authors.affiliation: Bioenergy Research Unit, National Center for Agricultural Research, USDA, Agricultural Research Service, 1815 N. University Street, Peoria, IL, 61604, USA. Electronic address: Terry.Whitehead@ars.usda.gov.
authors.forename: Terence R
authors.initials: TR
authors.lastname: Whitehead
authors.affiliation: General Studies, Gateway Technical College, Kenosha, WI, 53144, USA.
authors.forename: Christelle
authors.initials: C
authors.lastname: Anoma
authors.affiliation: General Studies, Gateway Technical College, Kenosha, WI, 53144, USA.
authors.forename: Richard W
authors.initials: RW
authors.lastname: McLaughlin
mesh_terms: D000818:Animals; D015373:Bacterial Typing Techniques; D000068016:Eubacteriales / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D017931:DNA Primers / Q000235:genetics; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005060:Europe; D005243:Feces / Q000382:microbiology; D006801:Humans; D008372:Manure / Q000382:microbiology; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000662:veterinary; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D017422:Sequence Analysis, DNA; D013552:Swine; D014481:United States
chemical_list: D017931:DNA Primers; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D008372:Manure; D012336:RNA, Ribosomal, 16S
keywords: 16S rRNA; Assay; Gastrointestinal; PCR; Peoriensis; Robinsoniella