title: Overcoming drug resistance with on-demand charged thermoresponsive dendritic nanogels.
abstract: OBJECTIVE
  To develop nanogels (NG) able to modulate the encapsulation and release of drugs, in order to circumvent drug resistance mechanisms in cancer cells.
  
  
  METHODS
  Poly-N-isopropylacrylamide-dendritic polyglycerol NG were semi-interpenetrated with 2-acrylamido-2-methylpropane sulfonic acid or (2-dimethylamino) ethyl methacrylate. Physico-chemical properties of the NGs as well as doxorubicin (DOXO) loading and release were characterized. Drug delivery performance was investigated in vitro and in vivo in a multidrug-resistant tumor model.
  
  
  RESULTS
  Both the DOXO loaded semi-interpenetrating polymer network NGs were more efficient in multidrug resistant cancer cell proliferation inhibition studies. In vivo, the DOXO loaded NG semi-interpenetrated with 2-acrylamido-2-methylpropane sulfonic acid was able to overcome drug resistance and reduce the tumor volume to about 25%.
  
  
  CONCLUSIONS
  The innovative semi-interpenetrating polymer network NGs appear to be promising drug carriers for drug resistant cancer therapy.
authors.affiliation: Institute for Chemistry & Biochemistry, Freie Universität Berlin, Takustr. 3, 14195 Berlin, Germany.
authors.forename: Maria
authors.initials: M
authors.lastname: Molina
authors.affiliation: Institute for Chemistry & Biochemistry, Freie Universität Berlin, Takustr. 3, 14195 Berlin, Germany.
authors.forename: Stefanie
authors.initials: S
authors.lastname: Wedepohl
authors.affiliation: Institute for Chemistry & Biochemistry, Freie Universität Berlin, Takustr. 3, 14195 Berlin, Germany.|Helmholtz Virtual Institute "Multifunctional Biomaterials for Medicine", Kantstr. 55, 14513 Teltow, Germany.
authors.forename: Enrico
authors.initials: E
authors.lastname: Miceli
authors.affiliation: Institute for Chemistry & Biochemistry, Freie Universität Berlin, Takustr. 3, 14195 Berlin, Germany.|Helmholtz Virtual Institute "Multifunctional Biomaterials for Medicine", Kantstr. 55, 14513 Teltow, Germany.
authors.forename: Marcelo
authors.initials: M
authors.lastname: Calderón
mesh_terms: D000180:Acrylic Resins / Q000737:chemistry; D004317:Doxorubicin / Q000737:chemistry; D004337:Drug Carriers / Q000737:chemistry*; D016503:Drug Delivery Systems / Q000379:methods; D004351:Drug Resistance; D000080385:Nanogels; D011092:Polyethylene Glycols / Q000737:chemistry*; D011094:Polyethyleneimine / Q000737:chemistry*
chemical_list: D000180:Acrylic Resins; D004337:Drug Carriers; D000080385:Nanogels; C413692:polyethylene glycol polyethyleneimine nanogel; C052970:poly-N-isopropylacrylamide; D011092:Polyethylene Glycols; D004317:Doxorubicin; D011094:Polyethyleneimine
keywords: doxorubicin carrier; drug resistance; semi-interpenetrated PNIPAM