title: Helicobacter pylori outer membrane protein, HomC, shows geographic dependent polymorphism that is influenced by the Bab family.
abstract: The array of outer membrane proteins (OMPs) found in Helicobacter pylori provides a crucial component for persistent colonization within the gastric niche. Not only does H. pylori harbor a wide number of OMPs, but these OMPs often vary across strains; this likely contributes to immune evasion, adaptation during long term colonization, and potentially differential disease progression. Previous work from our group described OMP differences among the Bab family (babA, babB, and babC) and Hom family (homA and homB) from 80 American H. pylori clinical isolates (AH) and 80 South Korean H. pylori clinical isolates (KH). In the current study, we expanded our investigation to include the less well characterized Hom family member, HomC.Overall, we identified and genotyped three homC variants: homCS, homCL, and homCM, in both populations. Similar to other polymorphic genes, the KH group showed less overall diversity, with 97.5% of strains harboring homCL. In contrast, a more heterogeneous profile was observed in strains derived from an American population; we found nearly equal distribution of homCSand homCL. Further analysis of the AH group identified associations between homC polymorphism and bab genotype; in AH strains, there was a significant association between homCLand carriage of babA at locus A. Since babA is an important virulence factor for the development of severe gastric disease, these data may suggest that homC polymorphism plays a role in H. pylori pathogenesis.
authors.affiliation: Department of Oral Biology, Oral Science Research Center, BK21 Plus Project, Yonsei University College of Dentistry, Seoul, 03722, Republic of Korea.|Department of Applied Life Science, The Graduate School, Yonsei University, Seoul, 03722, Republic of Korea.
authors.forename: Aeryun
authors.initials: A
authors.lastname: Kim
authors.affiliation: Department of Microbiology and Immunology, Uniformed Services University of the Health Sciences, 4301 Jones Bridge Rd., Bethesda, MD, 20814, USA.
authors.forename: Stephanie L
authors.initials: SL
authors.lastname: Servetas
authors.affiliation: Department of Oral Biology, Oral Science Research Center, BK21 Plus Project, Yonsei University College of Dentistry, Seoul, 03722, Republic of Korea.|Department of Applied Life Science, The Graduate School, Yonsei University, Seoul, 03722, Republic of Korea.
authors.forename: Jieun
authors.initials: J
authors.lastname: Kang
authors.affiliation: Department of Oral Biology, Oral Science Research Center, BK21 Plus Project, Yonsei University College of Dentistry, Seoul, 03722, Republic of Korea.
authors.forename: Jinmoon
authors.initials: J
authors.lastname: Kim
authors.affiliation: Department of Oral Biology, Oral Science Research Center, BK21 Plus Project, Yonsei University College of Dentistry, Seoul, 03722, Republic of Korea.|Department of Applied Life Science, The Graduate School, Yonsei University, Seoul, 03722, Republic of Korea.
authors.forename: Sungil
authors.initials: S
authors.lastname: Jang
authors.affiliation: Department of Oral Biology, Oral Science Research Center, BK21 Plus Project, Yonsei University College of Dentistry, Seoul, 03722, Republic of Korea.|Department of Applied Life Science, The Graduate School, Yonsei University, Seoul, 03722, Republic of Korea.
authors.forename: Yun Hui
authors.initials: YH
authors.lastname: Choi
authors.affiliation: Department of Oral Biology, Oral Science Research Center, BK21 Plus Project, Yonsei University College of Dentistry, Seoul, 03722, Republic of Korea.|Department of Applied Life Science, The Graduate School, Yonsei University, Seoul, 03722, Republic of Korea.
authors.forename: Hanfu
authors.initials: H
authors.lastname: Su
authors.affiliation: Department of Oral Biology, Oral Science Research Center, BK21 Plus Project, Yonsei University College of Dentistry, Seoul, 03722, Republic of Korea.
authors.forename: Yeong-Eui
authors.initials: YE
authors.lastname: Jeon
authors.affiliation: Department of Oral Biology, Oral Science Research Center, BK21 Plus Project, Yonsei University College of Dentistry, Seoul, 03722, Republic of Korea.|Department of Applied Life Science, The Graduate School, Yonsei University, Seoul, 03722, Republic of Korea.
authors.forename: Youngmin A
authors.initials: YA
authors.lastname: Hong
authors.affiliation: Department of Oral Biology, Oral Science Research Center, BK21 Plus Project, Yonsei University College of Dentistry, Seoul, 03722, Republic of Korea.|Department of Applied Life Science, The Graduate School, Yonsei University, Seoul, 03722, Republic of Korea.
authors.forename: Yun-Jung
authors.initials: YJ
authors.lastname: Yoo
authors.affiliation: Department of Microbiology and Immunology, Uniformed Services University of the Health Sciences, 4301 Jones Bridge Rd., Bethesda, MD, 20814, USA. douglas.merrell@usuhs.edu.
authors.forename: D Scott
authors.initials: DS
authors.lastname: Merrell
authors.affiliation: Department of Oral Biology, Oral Science Research Center, BK21 Plus Project, Yonsei University College of Dentistry, Seoul, 03722, Republic of Korea. jcha@yuhs.ac.|Department of Applied Life Science, The Graduate School, Yonsei University, Seoul, 03722, Republic of Korea. jcha@yuhs.ac.|Microbiology and Molecular Biology Laboratory, Key Laboratory of Oral Medicine, Guangzhou Institute of Oral Disease, Stomatology Hospital of Guangzhou Medical University, Guangzhou, P. R. China. jcha@yuhs.ac.
authors.forename: Jeong-Heon
authors.initials: JH
authors.lastname: Cha
mesh_terms: D018829:Adhesins, Bacterial; D000595:Amino Acid Sequence; D001425:Bacterial Outer Membrane Proteins / Q000737:chemistry / Q000235:genetics*; D014644:Genetic Variation; D005838:Genotype; D016481:Helicobacter Infections / Q000382:microbiology; D016480:Helicobacter pylori / Q000145:classification / Q000235:genetics* / Q000472:pathogenicity; D006801:Humans; D011110:Polymorphism, Genetic; D037521:Virulence Factors
chemical_list: D018829:Adhesins, Bacterial; C451032:BabB protein, Helicobacter pylori; D001425:Bacterial Outer Membrane Proteins; D037521:Virulence Factors
keywords: Helicobacter pylori; OMP; babA; babB; babC; homC