title: Prevalence of periodontal pathogens as predictor of the evolution of periodontal status.
abstract: The aim of this study was to determine the relationship between the prevalence of Porphyromonas gingivalis, its fimA genotypes, Aggregatibacter actinomycetemcomitans, Tannerella forsythia, and Treponema denticola and the evolution of periodontal health. In a longitudinal prospective study, samples of subgingival plaque were taken from 114 patients (37 with chronic periodontitis, 17 with gingivitis, and 60 periodontally healthy) in the course of a full periodontal examination. PCR was employed to determine the presence of the periodontopathogenic bacteria. Four years later, a second examination and sample collection were performed in 90 of these patients (20 with chronic periodontitis, 12 with gingivitis, and 58 periodontally healthy). T. forsythia, P. gingivalis, and T. denticola are the most prevalent bacteria in patients with chronic periodontitis (78.4%, 62.2 y 56.8%, respectively). The P. gingivalis bacterium and its fimA genotypes I, II, and IV showed the highest correlation between the baseline and follow-up assessments. P. gingivalis fimA genotype II and T. forsythia were associated to a significant degree with unfavourable periodontal evolution. Of the variables studied, P. gingivalis fimA genotype II and T. forsythia increase the risk of an unfavourable evolution of periodontal status.
authors.affiliation: Department of Stomatology, University of Valencia, Valencia, Spain. miriam.puig@uv.es.|Departamento de Estomatología, Universitat de València, Gascó Oliag no 1, 46010, Valencia, Spain. miriam.puig@uv.es.
authors.forename: Miriam
authors.initials: M
authors.lastname: Puig-Silla
authors.affiliation: Department of Stomatology, University of Valencia, Valencia, Spain.
authors.forename: José María
authors.initials: JM
authors.lastname: Montiel-Company
authors.affiliation: Fundación Investigación Hospital Clínico Universitario de Valencia/INCLIVA, Valencia, Spain.
authors.forename: Francisco
authors.initials: F
authors.lastname: Dasí-Fernández
authors.affiliation: Department of Stomatology, University of Valencia, Valencia, Spain.
authors.forename: José Manuel
authors.initials: JM
authors.lastname: Almerich-Silla
mesh_terms: D000328:Adult; D016976:Aggregatibacter actinomycetemcomitans / Q000302:isolation & purification*; D055113:Chronic Periodontitis / Q000382:microbiology*; D003773:Dental Plaque / Q000382:microbiology*; D005260:Female; D005891:Gingivitis / Q000382:microbiology*; D006801:Humans; D008137:Longitudinal Studies; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D016966:Porphyromonas gingivalis / Q000302:isolation & purification*; D015995:Prevalence; D011446:Prospective Studies; D000070436:Tannerella forsythia / Q000302:isolation & purification*; D048373:Treponema denticola / Q000302:isolation & purification*
keywords: FimA genotype; Periodontal bacteria; Periodontitis; Polymerase chain reaction; Porphyromonas gingivalis