title: Nitric oxide triggers a transient metabolic reprogramming in Arabidopsis.
abstract: Nitric oxide (NO) regulates plant growth and development as well as responses to stress that enhanced its endogenous production. Arabidopsis plants exposed to a pulse of exogenous NO gas were used for untargeted global metabolomic analyses thus allowing the identification of metabolic processes affected by NO. At early time points after treatment, NO scavenged superoxide anion and induced the nitration and the S-nitrosylation of proteins. These events preceded an extensive though transient metabolic reprogramming at 6 h after NO treatment, which included enhanced levels of polyamines, lipid catabolism and accumulation of phospholipids, chlorophyll breakdown, protein and nucleic acid turnover and increased content of sugars. Accordingly, lipid-related structures such as root cell membranes and leaf cuticle altered their permeability upon NO treatment. Besides, NO-treated plants displayed degradation of starch granules, which is consistent with the increased sugar content observed in the metabolomic survey. The metabolic profile was restored to baseline levels at 24 h post-treatment, thus pointing up the plasticity of plant metabolism in response to nitroxidative stress conditions.
authors.affiliation: Instituto de Biología Molecular y Celular de Plantas (Consejo Superior de Investigaciones Científicas-Universidad Politécnica de Valencia), CPI Edificio 8E, Avda. Ingeniero Fausto Elio s/n, 46022 Valencia, Spain.
authors.forename: José
authors.initials: J
authors.lastname: León
authors.affiliation: Instituto de Biología Molecular y Celular de Plantas (Consejo Superior de Investigaciones Científicas-Universidad Politécnica de Valencia), CPI Edificio 8E, Avda. Ingeniero Fausto Elio s/n, 46022 Valencia, Spain.
authors.forename: Álvaro
authors.initials: Á
authors.lastname: Costa
authors.affiliation: Instituto de Biología Molecular y Celular de Plantas (Consejo Superior de Investigaciones Científicas-Universidad Politécnica de Valencia), CPI Edificio 8E, Avda. Ingeniero Fausto Elio s/n, 46022 Valencia, Spain.
authors.forename: Mari-Cruz
authors.initials: MC
authors.lastname: Castillo
mesh_terms: D017360:Arabidopsis / Q000187:drug effects / Q000254:growth & development* / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000187:drug effects; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D055442:Metabolome / Q000187:drug effects*; D055432:Metabolomics / Q000379:methods*; D009569:Nitric Oxide / Q000494:pharmacology*; D018515:Plant Leaves / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism; D018517:Plant Roots / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism; D013481:Superoxides / Q000378:metabolism
chemical_list: D029681:Arabidopsis Proteins; D013481:Superoxides; D009569:Nitric Oxide