title: Simultaneous removal of structurally different pesticides in a biomixture: Detoxification and effect of oxytetracycline.
abstract: The biopurification systems (BPS) used for the treatment of pesticide-containing wastewater must present a versatile degrading ability, in order to remove different active ingredients according to the crop protection programs. This work aimed to assay the simultaneous removal of several pesticides (combinations of herbicides/insecticides/fungicides, or insecticides/fungicides) in a biomixture used in a BPS over a period of 115 d, and in the presence of oxytetracycline (OTC), an antibiotic of agricultural use that could be present in wastewater from agricultural pesticide application practices. The biomixture was able to mostly remove the herbicides during the treatment (removal rates: atrazine ≈ linuron > ametryn), and suffered no inhibition by OTC (only slightly for ametryn). Two fungicides (carbendazim and metalaxyl) were removed, nonetheless, in the systems containing only fungicides and insecticides, a clear increase in their half-lives was obtained in the treatments containing OTC. The neonicotinoid insecticides (imidacloprid and thiamethoxam) and the triazole fungicides (tebuconazole and triadimenol) were not significantly eliminated in the biomixture. Globally, the total removal of active ingredients ranged from 40.9% to 61.2% depending on the system, following the pattern: herbicides > fungicides > insecticides. The ecotoxicological analysis of the process revealed no detoxification towards the microcrustacean Daphnia magna, but a significant decay in the phytotoxicity towards Lactuca sativa in some cases, according to seed germination tests; in this case, OTC proved to be partially responsible for the phytotoxicity. The patterns of pesticide removal and detoxification provide inputs for the improvement of BPS use and their relevance as devices for wastewater treatment according to specific pesticide application programs.
authors.affiliation: Centro de Investigación en Contaminación Ambiental (CICA), Universidad de Costa Rica, 2060 San José, Costa Rica.
authors.forename: Alejandra
authors.initials: A
authors.lastname: Huete-Soto
authors.affiliation: Centro de Investigación en Contaminación Ambiental (CICA), Universidad de Costa Rica, 2060 San José, Costa Rica.
authors.forename: Mario
authors.initials: M
authors.lastname: Masís-Mora
authors.affiliation: Centro de Investigación en Contaminación Ambiental (CICA), Universidad de Costa Rica, 2060 San José, Costa Rica.
authors.forename: Verónica
authors.initials: V
authors.lastname: Lizano-Fallas
authors.affiliation: Centro de Investigación en Contaminación Ambiental (CICA), Universidad de Costa Rica, 2060 San José, Costa Rica.
authors.forename: Juan Salvador
authors.initials: JS
authors.lastname: Chin-Pampillo
authors.affiliation: Centro de Investigación en Contaminación Ambiental (CICA), Universidad de Costa Rica, 2060 San José, Costa Rica.
authors.forename: Elizabeth
authors.initials: E
authors.lastname: Carazo-Rojas
authors.affiliation: Centro de Investigación en Contaminación Ambiental (CICA), Universidad de Costa Rica, 2060 San José, Costa Rica. Electronic address: carlos.rodriguezrodriguez@ucr.ac.cr.
authors.forename: Carlos E
authors.initials: CE
authors.lastname: Rodríguez-Rodríguez
mesh_terms: D000383:Agriculture; D000818:Animals; D001673:Biodegradation, Environmental; D003621:Daphnia / Q000187:drug effects; D054750:Ecotoxicology; D005659:Fungicides, Industrial / Q000032:analysis / Q000737:chemistry / Q000633:toxicity; D018525:Germination / Q000187:drug effects; D006540:Herbicides / Q000032:analysis / Q000737:chemistry / Q000633:toxicity; D007306:Insecticides / Q000032:analysis / Q000737:chemistry; D018545:Lactuca / Q000187:drug effects / Q000254:growth & development; D010118:Oxytetracycline / Q000032:analysis* / Q000737:chemistry / Q000633:toxicity; D010573:Pesticide Residues / Q000032:analysis* / Q000737:chemistry / Q000633:toxicity; D012639:Seeds / Q000187:drug effects; D062065:Wastewater / Q000032:analysis / Q000737:chemistry* / Q000633:toxicity; D018508:Water Purification / Q000379:methods*
chemical_list: D005659:Fungicides, Industrial; D006540:Herbicides; D007306:Insecticides; D010118:Oxytetracycline; D010573:Pesticide Residues; D062065:Wastewater
keywords: Antibiotics; Biopurification system; Degradation; Pesticides; Toxicity