title: Informativeness of NGS Analysis for Vaginal Fluid Identification.
abstract: The identification of vaginal fluids in forensic examinations plays an important role in crime scene reconstruction. Molecular detection of vaginal bacterial communities can lead to the correct discrimination of body fluids. These kinds of studies can be performed through multiplex real-time PCR using primers for a specific selection of bacteria. The availability of next-generation sequencing (NGS) protocols provided for the extension of the analysis to evaluate the prokaryotes present in specimens. In this study, DNA was extracted from 18 samples (vaginal, oral, fecal, yoghurt) and analyzed by real-time PCR and NGS. The comparison between the two approaches has demonstrated that the information developed through NGS can augment the more conventional real-time PCR detection of a few key bacterial species to provide a more probative result and the correct identification of vaginal fluid from samples that are more forensically challenged.
authors.affiliation: Department of Movement, Human and Health Sciences, Public Health Unit, University of Rome "Foro Italico", Piazza Lauro De Bosis, 6, 00135, Rome, Italy.
authors.forename: Saverio
authors.initials: S
authors.lastname: Giampaoli
authors.affiliation: Department of Movement, Human and Health Sciences, Public Health Unit, University of Rome "Foro Italico", Piazza Lauro De Bosis, 6, 00135, Rome, Italy.
authors.forename: Elisabetta
authors.initials: E
authors.lastname: DeVittori
authors.affiliation: Department of Movement, Human and Health Sciences, Public Health Unit, University of Rome "Foro Italico", Piazza Lauro De Bosis, 6, 00135, Rome, Italy.
authors.forename: Federica
authors.initials: F
authors.lastname: Valeriani
authors.affiliation: Reparto Investigazioni Scientifiche di Roma, Carabinieri, Viale di Tor di Quinto, 119, 00191, Rome, Italy.
authors.forename: Andrea
authors.initials: A
authors.lastname: Berti
authors.affiliation: Department of Movement, Human and Health Sciences, Public Health Unit, University of Rome "Foro Italico", Piazza Lauro De Bosis, 6, 00135, Rome, Italy.
authors.forename: Vincenzo
authors.initials: V
authors.lastname: Romano Spica
mesh_terms: D002582:Cervix Mucus / Q000382:microbiology*; D004269:DNA, Bacterial / Q000235:genetics; D005243:Feces / Q000382:microbiology; D005260:Female; D053803:Forensic Genetics; D059014:High-Throughput Nucleotide Sequencing; D006801:Humans; D060888:Real-Time Polymerase Chain Reaction; D012463:Saliva / Q000382:microbiology; D014621:Vagina / Q000382:microbiology*
chemical_list: D004269:DNA, Bacterial
keywords: bacteria; body fluids; forensic science; metabarcoding; mfDNA; multiplex PCR