title: Zinc(II) ion promotes anti-inflammatory effects of rhSOD3 by increasing cellular association.
abstract: Recently, we demonstrated that superoxide dismutase 3 (SOD3) is a strong candidate for biomedicine. Anti-oxidant function of SOD3 was accomplished without cell penetration, and it inhibited the inflammatory responses via non-enzymatic functions. SOD3 has the heparin binding domain associating cell surface. Interestingly, we found that Zn2＋ promotes transduction effects of recombinant human SOD3 (rhSOD3) by increasing uptake via the heparin binding domain (HBD). We demonstrated an uptake of rhSOD3 from media to cell lysate via HBD, resulting in an accumulation of rhSOD3 in the nucleus, which was promoted by the presence of Zn2＋. This resulted in increased inhibitory effects of rhSOD3 on NF-kB and STAT3 signals in the presence of Zn2＋, which shows elevated association of rhSOD3 into the cells. These results suggest that an optimized procedure can help to enhance the inflammatory efficacy of rhSOD3, as a novel biomedicine. [BMB Reports 2017; 50(2): 85-90].
authors.affiliation: Department of Emergency Medical Technology, Kyungil University, Gyeongsan 38428, Korea.
authors.forename: Younghwa
authors.initials: Y
authors.lastname: Kim
authors.affiliation: Laboratory of Dermato-Immunology, Catholic Research Institute of Medical Science, College of Medicine, The Catholic University of Korea, Seoul 06591, Korea.
authors.forename: Yoon-Jae
authors.initials: YJ
authors.lastname: Jeon
authors.affiliation: RNS BIO, Seoul 04784, Korea.
authors.forename: Kang
authors.initials: K
authors.lastname: Ryu
authors.affiliation: Laboratory of Dermato-Immunology, Catholic Research Institute of Medical Science, College of Medicine, The Catholic University of Korea, Seoul 06591, Korea.
authors.forename: Tae-Yoon
authors.initials: TY
authors.lastname: Kim
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000493:pharmacokinetics / Q000494:pharmacology*; D002477:Cells / Q000187:drug effects* / Q000378:metabolism*; D002478:Cells, Cultured; D004357:Drug Synergism; D057809:HEK293 Cells; D006801:Humans; D051379:Mice; D011485:Protein Binding / Q000187:drug effects; D011994:Recombinant Proteins / Q000493:pharmacokinetics / Q000494:pharmacology*; D013482:Superoxide Dismutase / Q000493:pharmacokinetics / Q000494:pharmacology*; D015032:Zinc / Q000494:pharmacology*
chemical_list: D000893:Anti-Inflammatory Agents; D011994:Recombinant Proteins; C441642:SOD3 protein, human; D013482:Superoxide Dismutase; D015032:Zinc