title: miR-429 is involved in regulation of NF-κBactivity by targeting IKKβ and suppresses oncogenic activity in cervical cancer cells.
abstract: Dysregulation of microRNAs (miRNAs) can contribute to tumorigenesis in cancers. In this study, we found that miR-429 was downregulated in cervical cancer (CC) tissues and suppressed cell viability and proliferation while promoting apoptosis in CC cells. IKKβ was a novel target gene of miR-429 and ectopic expression of IKKβ abrogated the phenotypes induced by miR-429. When IKKβ was downregulated by miR-429, nuclear factor κB (NF-κB) pathway activation, interleukin-6 (IL-6), and interferon-β (IFN-β) production were decreased in CC cells. These findings indicate that miR-429 is involved in regulation of the NF-κB pathway by targeting IKKβ and functions as a tumor suppressor in cervical carcinogenesis.
authors.affiliation: Tianjin Life Science Research Center and Department of Pathogen Biology, School of Basic Medical Sciences, Tianjin Medical University, China.
authors.forename: Jing-Yi
authors.initials: JY
authors.lastname: Fan
authors.affiliation: Tianjin Life Science Research Center and Department of Pathogen Biology, School of Basic Medical Sciences, Tianjin Medical University, China.
authors.forename: Ya-Jie
authors.initials: YJ
authors.lastname: Fan
authors.affiliation: Tianjin Life Science Research Center and Department of Pathogen Biology, School of Basic Medical Sciences, Tianjin Medical University, China.
authors.forename: Xiang-Ling
authors.initials: XL
authors.lastname: Wang
authors.affiliation: Tianjin Life Science Research Center and Department of Pathogen Biology, School of Basic Medical Sciences, Tianjin Medical University, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Xie
authors.affiliation: Tianjin Life Science Research Center and Department of Pathogen Biology, School of Basic Medical Sciences, Tianjin Medical University, China.
authors.forename: Hui-Jie
authors.initials: HJ
authors.lastname: Gao
authors.affiliation: Tianjin Life Science Research Center and Department of Pathogen Biology, School of Basic Medical Sciences, Tianjin Medical University, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Tianjin Life Science Research Center and Department of Pathogen Biology, School of Basic Medical Sciences, Tianjin Medical University, China.
authors.forename: Min
authors.initials: M
authors.lastname: Liu
authors.affiliation: Tianjin Life Science Research Center and Department of Pathogen Biology, School of Basic Medical Sciences, Tianjin Medical University, China.
authors.forename: Hua
authors.initials: H
authors.lastname: Tang
mesh_terms: D017209:Apoptosis / Q000235:genetics; D001483:Base Sequence; D063646:Carcinogenesis / Q000235:genetics / Q000473:pathology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D002470:Cell Survival; D015536:Down-Regulation / Q000235:genetics; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D051550:I-kappa B Kinase / Q000378:metabolism*; D016899:Interferon-beta / Q000378:metabolism; D015850:Interleukin-6 / Q000378:metabolism; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D016328:NF-kappa B / Q000378:metabolism*; D002583:Uterine Cervical Neoplasms / Q000235:genetics* / Q000473:pathology
chemical_list: D015850:Interleukin-6; C546363:MIRN429 microRNA, human; D035683:MicroRNAs; D016328:NF-kappa B; D016899:Interferon-beta; D051550:I-kappa B Kinase
keywords: IKKβ; NF-κB; cervical cancer; miR-429