title: Multivalent Aptamers: Versatile Tools for Diagnostic and Therapeutic Applications.
abstract: Nucleic acid aptamers generated through an in vitro selection are currently extensively applied as very valuable biomolecular tools thanks to their prominent advantages. Diversity of spatial structures, ease of production through chemical synthesis and a large variety of chemical modifications make aptamers convenient building blocks for the generation of multifunctional constructs. An opportunity to combine different aptamer functionalities with other molecules of interest such as reporter groups, nanoparticles, chemotherapeutic agents, siRNA or antisense oligonucleotides provides a widest range of applications of multivalent aptamers. The present review summarizes approaches to the design of multivalent aptamers, various examples of multifunctional constructs and the prospects of employing them as components of biosensors, probes for affinity capture, tools for cell research and potential therapeutic candidates.
authors.affiliation: Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of the Russian Academy of Sciences, Lavrentiev Ave., 8, 630090 Novosibirsk, Russia. maria.vorobjeva@gmail.com.
authors.forename: Mariya
authors.initials: M
authors.lastname: Vorobyeva
authors.affiliation: Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of the Russian Academy of Sciences, Lavrentiev Ave., 8, 630090 Novosibirsk, Russia. vorobyev@niboch.nsc.ru.
authors.forename: Pavel
authors.initials: P
authors.lastname: Vorobjev
authors.affiliation: Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of the Russian Academy of Sciences, Lavrentiev Ave., 8, 630090 Novosibirsk, Russia. ven@niboch.nsc.ru.
authors.forename: Alya
authors.initials: A
authors.lastname: Venyaminova
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000737:chemistry / Q000494:pharmacology; D000970:Antineoplastic Agents / Q000737:chemistry / Q000494:pharmacology; D052157:Aptamers, Nucleotide / Q000737:chemistry*; D003933:Diagnosis; D006801:Humans; D011485:Protein Binding; D052156:SELEX Aptamer Technique; D013812:Therapeutics
chemical_list: D000893:Anti-Inflammatory Agents; D000970:Antineoplastic Agents; D052157:Aptamers, Nucleotide
keywords: SELEX; application; aptamers; conjugates; design; multivalency