title: Desulfobulbus oligotrophicus sp. nov., a sulfate-reducing and propionate-oxidizing bacterium isolated from a municipal anaerobic sewage sludge digester.
abstract: A novel, mesophilic, strictly anaerobic, sulfate-reducing and propionate-oxidizing bacterium, strain Prop6T, was enriched and isolated from a municipal anaerobic sewage sludge digester. Cells were Gram-stain-negative, catalase-positive, oval rods, motile by means of amphitrichous flagella, non-spore-forming and contained menaquinone MK-5(H2) as the major respiratory quinone. The genomic DNA G+C content was 51.7 mol%. The optimal NaCl concentration, temperature and pH were 2-5 g l-1, 35 °C and pH 7.6, respectively. Strain Prop6T could only oxidize propionate, lactate and pyruvate (weakly) with sulfate, sulfite or thiosulfate, mainly to acetate. Strain Prop6T fermented pyruvate and lactate to acetate and propionate. The predominant cellular fatty acids were C14 : 0, C16 : 0, C16 : 1ω7, C16 : 1ω5, C17 : 1ω6 and C18 : 1ω7. Phylogenetic analysis based on 16S rRNA gene sequences revealed that the newly isolated strain was a member of the genus Desulfobulbus, with Desulfobulbus elongatus DSM 2908T, Desulfobulbus propionicus DSM 2032T and Desulfobulbus rhabdoformis DSM 8777T as closest relatives among species with validly published names. On the basis of genotypic, phenotypic and chemotaxonomic characteristics, it is proposed that the isolate represents a novel species, Desulfobulbus oligotrophicus sp. nov. The type strain is Prop6T (=DSM 103420T=JCM 31535T).
authors.affiliation: Université de Pau et des Pays de l'Adour, CNRS, IPREM UMR 5254, Equipe Environnement et Microbiologie, 64013 Pau, France.|Anaerobic Microbiology Team (E02B26), Sciences and Techniques Faculty, Cadi Ayyad University PO Box 549, 40 000 Marrakech, Morocco.
authors.forename: Abdelaziz
authors.initials: A
authors.lastname: El Houari
authors.affiliation: Université de Pau et des Pays de l'Adour, CNRS, IPREM UMR 5254, Equipe Environnement et Microbiologie, 64013 Pau, France.
authors.forename: Magali
authors.initials: M
authors.lastname: Ranchou-Peyruse
authors.affiliation: Université de Pau et des Pays de l'Adour, CNRS, IPREM UMR 5254, Equipe Environnement et Microbiologie, 64013 Pau, France.
authors.forename: Anthony
authors.initials: A
authors.lastname: Ranchou-Peyruse
authors.affiliation: Université de Pau et des Pays de l'Adour, CNRS, IPREM UMR 5254, Equipe Environnement et Microbiologie, 64013 Pau, France.
authors.forename: Adrien
authors.initials: A
authors.lastname: Dakdaki
authors.affiliation: Université de Pau et des Pays de l'Adour, CNRS, IPREM UMR 5254, Equipe Environnement et Microbiologie, 64013 Pau, France.
authors.forename: Marion
authors.initials: M
authors.lastname: Guignard
authors.affiliation: Anaerobic Microbiology Team (E02B26), Sciences and Techniques Faculty, Cadi Ayyad University PO Box 549, 40 000 Marrakech, Morocco.
authors.forename: Lahcen
authors.initials: L
authors.lastname: Idouhammou
authors.affiliation: Anaerobic Microbiology Team (E02B26), Sciences and Techniques Faculty, Cadi Ayyad University PO Box 549, 40 000 Marrakech, Morocco.
authors.forename: Rhizlane
authors.initials: R
authors.lastname: Bennisse
authors.affiliation: Anaerobic Microbiology Team (E02B26), Sciences and Techniques Faculty, Cadi Ayyad University PO Box 549, 40 000 Marrakech, Morocco.
authors.forename: Radia
authors.initials: R
authors.lastname: Bouterfass
authors.affiliation: Université de Pau et des Pays de l'Adour, CNRS, IPREM UMR 5254, Equipe Environnement et Microbiologie, 64013 Pau, France.
authors.forename: Rémy
authors.initials: R
authors.lastname: Guyoneaud
authors.affiliation: Anaerobic Microbiology Team (E02B26), Sciences and Techniques Faculty, Cadi Ayyad University PO Box 549, 40 000 Marrakech, Morocco.
authors.forename: Abdel-Illah
authors.initials: AI
authors.lastname: Qatibi
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D020564:Deltaproteobacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D005227:Fatty Acids / Q000737:chemistry; D009018:Morocco; D010084:Oxidation-Reduction; D010802:Phylogeny; D011422:Propionates / Q000378:metabolism*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012722:Sewage / Q000382:microbiology*; D016999:Sulfur-Reducing Bacteria / Q000145:classification / Q000235:genetics / Q000302:isolation & purification
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D011422:Propionates; D012336:RNA, Ribosomal, 16S; D012722:Sewage