title: Natural Variation of Molecular and Morphological Gibberellin Responses.
abstract: Although phytohormones such as gibberellins are essential for many conserved aspects of plant physiology and development, plants vary greatly in their responses to these regulatory compounds. Here, we use genetic perturbation of endogenous gibberellin levels to probe the extent of intraspecific variation in gibberellin responses in natural accessions of Arabidopsis (Arabidopsis thaliana). We find that these accessions vary greatly in their ability to buffer the effects of overexpression of GA20ox1, encoding a rate-limiting enzyme for gibberellin biosynthesis, with substantial differences in bioactive gibberellin concentrations as well as transcriptomes and growth trajectories. These findings demonstrate a surprising level of flexibility in the wiring of regulatory networks underlying hormone metabolism and signaling.
authors.affiliation: Department of Plant Systems Biology, VIB, B-9052 Gent, Belgium (Y.J.N., D.H., F.C., V.S., T.V.D., S.D., D.I., N.G.).|Department of Plant Biotechnology and Bioinformatics, Ghent University, B-9052 Gent, Belgium (Y.J.N., D.H., F.C., V.S., T.V.D., S.D., D.I., N.G.).|Department of Molecular Biology, Max Planck Institute for Developmental Biology, 72076 Tübingen, Germany (E.C., D.W.); and.|RIKEN Center for Sustainable Resource Science, Tsurumi, Yokohama, Kanagawa 230-0045, Japan (M.K., H.S.).
authors.forename: Youn-Jeong
authors.identifier: 0000-0003-4018-3705
authors.initials: YJ
authors.lastname: Nam
authors.affiliation: Department of Plant Systems Biology, VIB, B-9052 Gent, Belgium (Y.J.N., D.H., F.C., V.S., T.V.D., S.D., D.I., N.G.).|Department of Plant Biotechnology and Bioinformatics, Ghent University, B-9052 Gent, Belgium (Y.J.N., D.H., F.C., V.S., T.V.D., S.D., D.I., N.G.).|Department of Molecular Biology, Max Planck Institute for Developmental Biology, 72076 Tübingen, Germany (E.C., D.W.); and.|RIKEN Center for Sustainable Resource Science, Tsurumi, Yokohama, Kanagawa 230-0045, Japan (M.K., H.S.).
authors.forename: Dorota
authors.initials: D
authors.lastname: Herman
authors.affiliation: Department of Plant Systems Biology, VIB, B-9052 Gent, Belgium (Y.J.N., D.H., F.C., V.S., T.V.D., S.D., D.I., N.G.).|Department of Plant Biotechnology and Bioinformatics, Ghent University, B-9052 Gent, Belgium (Y.J.N., D.H., F.C., V.S., T.V.D., S.D., D.I., N.G.).|Department of Molecular Biology, Max Planck Institute for Developmental Biology, 72076 Tübingen, Germany (E.C., D.W.); and.|RIKEN Center for Sustainable Resource Science, Tsurumi, Yokohama, Kanagawa 230-0045, Japan (M.K., H.S.).
authors.forename: Jonas
authors.identifier: 0000-0003-2941-0233
authors.initials: J
authors.lastname: Blomme
authors.affiliation: Department of Plant Systems Biology, VIB, B-9052 Gent, Belgium (Y.J.N., D.H., F.C., V.S., T.V.D., S.D., D.I., N.G.).|Department of Plant Biotechnology and Bioinformatics, Ghent University, B-9052 Gent, Belgium (Y.J.N., D.H., F.C., V.S., T.V.D., S.D., D.I., N.G.).|Department of Molecular Biology, Max Planck Institute for Developmental Biology, 72076 Tübingen, Germany (E.C., D.W.); and.|RIKEN Center for Sustainable Resource Science, Tsurumi, Yokohama, Kanagawa 230-0045, Japan (M.K., H.S.).
authors.forename: Eunyoung
authors.identifier: 0000-0002-0889-9837
authors.initials: E
authors.lastname: Chae
authors.affiliation: Department of Plant Systems Biology, VIB, B-9052 Gent, Belgium (Y.J.N., D.H., F.C., V.S., T.V.D., S.D., D.I., N.G.).|Department of Plant Biotechnology and Bioinformatics, Ghent University, B-9052 Gent, Belgium (Y.J.N., D.H., F.C., V.S., T.V.D., S.D., D.I., N.G.).|Department of Molecular Biology, Max Planck Institute for Developmental Biology, 72076 Tübingen, Germany (E.C., D.W.); and.|RIKEN Center for Sustainable Resource Science, Tsurumi, Yokohama, Kanagawa 230-0045, Japan (M.K., H.S.).
authors.forename: Mikiko
authors.initials: M
authors.lastname: Kojima
authors.affiliation: Department of Plant Systems Biology, VIB, B-9052 Gent, Belgium (Y.J.N., D.H., F.C., V.S., T.V.D., S.D., D.I., N.G.).|Department of Plant Biotechnology and Bioinformatics, Ghent University, B-9052 Gent, Belgium (Y.J.N., D.H., F.C., V.S., T.V.D., S.D., D.I., N.G.).|Department of Molecular Biology, Max Planck Institute for Developmental Biology, 72076 Tübingen, Germany (E.C., D.W.); and.|RIKEN Center for Sustainable Resource Science, Tsurumi, Yokohama, Kanagawa 230-0045, Japan (M.K., H.S.).
authors.forename: Frederik
authors.identifier: 0000-0001-6565-5145
authors.initials: F
authors.lastname: Coppens
authors.affiliation: Department of Plant Systems Biology, VIB, B-9052 Gent, Belgium (Y.J.N., D.H., F.C., V.S., T.V.D., S.D., D.I., N.G.).|Department of Plant Biotechnology and Bioinformatics, Ghent University, B-9052 Gent, Belgium (Y.J.N., D.H., F.C., V.S., T.V.D., S.D., D.I., N.G.).|Department of Molecular Biology, Max Planck Institute for Developmental Biology, 72076 Tübingen, Germany (E.C., D.W.); and.|RIKEN Center for Sustainable Resource Science, Tsurumi, Yokohama, Kanagawa 230-0045, Japan (M.K., H.S.).
authors.forename: Veronique
authors.identifier: 0000-0003-4762-6580
authors.initials: V
authors.lastname: Storme
authors.affiliation: Department of Plant Systems Biology, VIB, B-9052 Gent, Belgium (Y.J.N., D.H., F.C., V.S., T.V.D., S.D., D.I., N.G.).|Department of Plant Biotechnology and Bioinformatics, Ghent University, B-9052 Gent, Belgium (Y.J.N., D.H., F.C., V.S., T.V.D., S.D., D.I., N.G.).|Department of Molecular Biology, Max Planck Institute for Developmental Biology, 72076 Tübingen, Germany (E.C., D.W.); and.|RIKEN Center for Sustainable Resource Science, Tsurumi, Yokohama, Kanagawa 230-0045, Japan (M.K., H.S.).
authors.forename: Twiggy
authors.initials: T
authors.lastname: Van Daele
authors.affiliation: Department of Plant Systems Biology, VIB, B-9052 Gent, Belgium (Y.J.N., D.H., F.C., V.S., T.V.D., S.D., D.I., N.G.).|Department of Plant Biotechnology and Bioinformatics, Ghent University, B-9052 Gent, Belgium (Y.J.N., D.H., F.C., V.S., T.V.D., S.D., D.I., N.G.).|Department of Molecular Biology, Max Planck Institute for Developmental Biology, 72076 Tübingen, Germany (E.C., D.W.); and.|RIKEN Center for Sustainable Resource Science, Tsurumi, Yokohama, Kanagawa 230-0045, Japan (M.K., H.S.).
authors.forename: Stijn
authors.identifier: 0000-0003-4402-2191
authors.initials: S
authors.lastname: Dhondt
authors.affiliation: Department of Plant Systems Biology, VIB, B-9052 Gent, Belgium (Y.J.N., D.H., F.C., V.S., T.V.D., S.D., D.I., N.G.).|Department of Plant Biotechnology and Bioinformatics, Ghent University, B-9052 Gent, Belgium (Y.J.N., D.H., F.C., V.S., T.V.D., S.D., D.I., N.G.).|Department of Molecular Biology, Max Planck Institute for Developmental Biology, 72076 Tübingen, Germany (E.C., D.W.); and.|RIKEN Center for Sustainable Resource Science, Tsurumi, Yokohama, Kanagawa 230-0045, Japan (M.K., H.S.).
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Sakakibara
authors.affiliation: Department of Plant Systems Biology, VIB, B-9052 Gent, Belgium (Y.J.N., D.H., F.C., V.S., T.V.D., S.D., D.I., N.G.).|Department of Plant Biotechnology and Bioinformatics, Ghent University, B-9052 Gent, Belgium (Y.J.N., D.H., F.C., V.S., T.V.D., S.D., D.I., N.G.).|Department of Molecular Biology, Max Planck Institute for Developmental Biology, 72076 Tübingen, Germany (E.C., D.W.); and.|RIKEN Center for Sustainable Resource Science, Tsurumi, Yokohama, Kanagawa 230-0045, Japan (M.K., H.S.).
authors.forename: Detlef
authors.identifier: 0000-0002-2114-7963
authors.initials: D
authors.lastname: Weigel
authors.affiliation: Department of Plant Systems Biology, VIB, B-9052 Gent, Belgium (Y.J.N., D.H., F.C., V.S., T.V.D., S.D., D.I., N.G.); dirk.inze@psb.vib-ugent.be.|Department of Plant Biotechnology and Bioinformatics, Ghent University, B-9052 Gent, Belgium (Y.J.N., D.H., F.C., V.S., T.V.D., S.D., D.I., N.G.); dirk.inze@psb.vib-ugent.be.|Department of Molecular Biology, Max Planck Institute for Developmental Biology, 72076 Tübingen, Germany (E.C., D.W.); and dirk.inze@psb.vib-ugent.be.|RIKEN Center for Sustainable Resource Science, Tsurumi, Yokohama, Kanagawa 230-0045, Japan (M.K., H.S.) dirk.inze@psb.vib-ugent.be.
authors.forename: Dirk
authors.identifier: 0000-0002-3217-8407
authors.initials: D
authors.lastname: Inzé
authors.affiliation: Department of Plant Systems Biology, VIB, B-9052 Gent, Belgium (Y.J.N., D.H., F.C., V.S., T.V.D., S.D., D.I., N.G.).|Department of Plant Biotechnology and Bioinformatics, Ghent University, B-9052 Gent, Belgium (Y.J.N., D.H., F.C., V.S., T.V.D., S.D., D.I., N.G.).|Department of Molecular Biology, Max Planck Institute for Developmental Biology, 72076 Tübingen, Germany (E.C., D.W.); and.|RIKEN Center for Sustainable Resource Science, Tsurumi, Yokohama, Kanagawa 230-0045, Japan (M.K., H.S.).
authors.forename: Nathalie
authors.identifier: 0000-0002-3946-1758
authors.initials: N
authors.lastname: Gonzalez
mesh_terms: D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D018506:Gene Expression Regulation, Plant; D014644:Genetic Variation; D005875:Gibberellins / Q000378:metabolism*; D006899:Mixed Function Oxygenases / Q000235:genetics / Q000378:metabolism*; D010937:Plant Growth Regulators / Q000378:metabolism; D018515:Plant Leaves / Q000235:genetics / Q000254:growth & development; D030821:Plants, Genetically Modified
chemical_list: D029681:Arabidopsis Proteins; D005875:Gibberellins; D010937:Plant Growth Regulators; D006899:Mixed Function Oxygenases; C000606235:gibberellin 20 oxidase 1, Arabidopsis