title: Sequence-specific recognition of methylated DNA by an engineered transcription activator-like effector protein.
abstract: A 5mC-selective TALE-repeat was created by screening a TALE repeat library containing randomized amino acids at repeat variable diresidues and their neighboring residues. The new repeat showed high 5mC discrimination ability. An artificial TALE containing the new repeat activated an endogenous gene in a genomic methylation status-dependent manner.
authors.affiliation: Institute for Chemical Research, Kyoto University, Uji, Kyoto 611-0011, Japan. imiki@scl.kyoto-u.ac.jp.
authors.forename: Shogo
authors.initials: S
authors.lastname: Tsuji
authors.affiliation: Institute for Chemical Research, Kyoto University, Uji, Kyoto 611-0011, Japan. imiki@scl.kyoto-u.ac.jp.
authors.forename: Shiroh
authors.initials: S
authors.lastname: Futaki
authors.affiliation: Institute for Chemical Research, Kyoto University, Uji, Kyoto 611-0011, Japan. imiki@scl.kyoto-u.ac.jp.
authors.forename: Miki
authors.initials: M
authors.lastname: Imanishi
mesh_terms: D000595:Amino Acid Sequence; D000596:Amino Acids / Q000737:chemistry / Q000235:genetics*; D019175:DNA Methylation / Q000235:genetics*; D005818:Genetic Engineering; D000071336:Transcription Activator-Like Effectors / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism
chemical_list: D000596:Amino Acids; D000071336:Transcription Activator-Like Effectors