title: Impact of Immune Deficiency on Remodeling of Maternal Resistance Vasculature 4 Weeks Postpartum in Mice.
abstract: Pregnancy manifests changes in the vascular and immune systems that persist postpartum (PP), have important implications for future pregnancies, and may modify responses to cardiovascular stress in late life. The association between immune and vascular function and the generation or progression of cardiovascular disease beg the question of whether altered immunity modifies pregnancy-induced changes in the vasculature. Our objective was to compare changes in the function and remodeling of systemic resistance vessels 4 weeks PP in normal C57BL/6 (B6), and immunodeficient mice recombinase 1-deficient/B6 ( Rag1-/-). Immune deficiency did not change the responsiveness to acetylcholine (ACh) and phenylephrine at baseline but decreased arterial distensibility and increased stiffness PP. Adoptive transfer of CD8 T cells into Rag1-/-mice decreased the response to ACh while increasing distensibility and wall thickness. When compared to PP Rag1-/-, vessels from PP CD4-deficient mice, which have B cells and CD8 T cells, but no CD4 cells, show increased distensibility and decreased responsiveness to ACh in a pattern similar to that seen in Rag1-/-given CD8 T cells prior to mating. These studies suggest a key role for T cell, particularly CD8 T cell, associated factors in the PP remodeling of maternal resistance vessels.
authors.affiliation: 1 Division of Reproductive Science Research, Department of Obstetrics, Gynecology and Reproductive Sciences, University of Vermont, Burlington, VT, USA.
authors.forename: Elizabeth A
authors.initials: EA
authors.lastname: Bonney
authors.affiliation: 1 Division of Reproductive Science Research, Department of Obstetrics, Gynecology and Reproductive Sciences, University of Vermont, Burlington, VT, USA.
authors.forename: Ann
authors.initials: A
authors.lastname: Howard
authors.affiliation: 1 Division of Reproductive Science Research, Department of Obstetrics, Gynecology and Reproductive Sciences, University of Vermont, Burlington, VT, USA.
authors.forename: Kendall
authors.initials: K
authors.lastname: Krebs
authors.affiliation: 1 Division of Reproductive Science Research, Department of Obstetrics, Gynecology and Reproductive Sciences, University of Vermont, Burlington, VT, USA.
authors.forename: Kelly
authors.initials: K
authors.lastname: Begin
authors.affiliation: 1 Division of Reproductive Science Research, Department of Obstetrics, Gynecology and Reproductive Sciences, University of Vermont, Burlington, VT, USA.
authors.forename: Kelsey
authors.initials: K
authors.lastname: Veilleux
authors.affiliation: 1 Division of Reproductive Science Research, Department of Obstetrics, Gynecology and Reproductive Sciences, University of Vermont, Burlington, VT, USA.
authors.forename: Natalia I
authors.initials: NI
authors.lastname: Gokina
mesh_terms: D000109:Acetylcholine / Q000494:pharmacology; D019264:Adoptive Transfer; D000818:Animals; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology*; D005260:Female; D016654:Genes, RAG-1 / Q000235:genetics; D051379:Mice; D018345:Mice, Knockout; D010656:Phenylephrine / Q000494:pharmacology; D049590:Postpartum Period; D011247:Pregnancy; D014655:Vascular Resistance / Q000187:drug effects / Q000276:immunology*
chemical_list: D010656:Phenylephrine; D000109:Acetylcholine
keywords: B cells; T cells; mice; postpartum; pregnancy; vascular remodeling