title: A Detailed Analysis of theBR1Locus Suggests a New Mechanism for Bolting after Winter in Sugar Beet (Beta vulgarisL.).
abstract: Sugar beet (Beta vulgarisssp. vulgaris) is a biennial, sucrose-storing plant, which is mainly cultivated as a spring crop and harvested in the vegetative stage before winter. For increasing beet yield, over-winter cultivation would be advantageous. However, bolting is induced after winter and drastically reduces yield. Thus, post-winter bolting control is essential for winter beet cultivation. To identify genetic factors controlling bolting after winter, a F2population was previously developed by crossing the sugar beet accessions BETA 1773 with reduced bolting tendency and 93161P with complete bolting after winter. For a mapping-by-sequencing analysis, pools of 26 bolting-resistant and 297 bolting F2plants were used. Thereby, a single continuous homozygous region of 103 kb was co-localized to the previously publishedBR1QTL for post-winter bolting resistance (Pfeiffer et al., 2014). TheBR1locus was narrowed down to 11 candidate genes from which a homolog of theArabidopsis CLEAVAGE AND POLYADENYLATION SPECIFICITY FACTOR 73-I(CPSF73-I) was identified as the most promising candidate. A 2 bp deletion within the BETA 1773 allele ofBvCPSF73-Iaresults in a truncated protein. However, the null allele ofBvCPSF73-Iamight partially be compensated by a secondBvCPSF73-Ibgene. This gene is located 954 bp upstream ofBvCPSF73-Iaand could be responsible for the incomplete penetrance of the post-winter bolting resistance allele of BETA 1773. This result is an important milestone for breeding winter beets with complete bolting resistance after winter.
authors.affiliation: Plant Breeding Institute, University of Kiel Kiel, Germany.
authors.forename: Conny
authors.initials: C
authors.lastname: Tränkner
authors.affiliation: Institute of Computer Science, Martin Luther University Halle-Wittenberg Halle, Germany.
authors.forename: Ioana M
authors.initials: IM
authors.lastname: Lemnian
authors.affiliation: Plant Breeding Institute, University of Kiel Kiel, Germany.
authors.forename: Nazgol
authors.initials: N
authors.lastname: Emrani
authors.affiliation: Plant Breeding Institute, University of Kiel Kiel, Germany.
authors.forename: Nina
authors.initials: N
authors.lastname: Pfeiffer
authors.affiliation: Plant Breeding Institute, University of Kiel Kiel, Germany.
authors.forename: Surya P
authors.initials: SP
authors.lastname: Tiwari
authors.affiliation: Plant Breeding Institute, University of Kiel Kiel, Germany.
authors.forename: Friedrich J
authors.initials: FJ
authors.lastname: Kopisch-Obuch
authors.affiliation: Plant Breeding Institute, University of Kiel Kiel, Germany.
authors.forename: Sebastian H
authors.initials: SH
authors.lastname: Vogt
authors.affiliation: Plant Breeding Institute, University of Kiel Kiel, Germany.
authors.forename: Andreas E
authors.initials: AE
authors.lastname: Müller
authors.affiliation: Institute of Clinical Molecular Biology, University of Kiel Kiel, Germany.
authors.forename: Markus
authors.initials: M
authors.lastname: Schilhabel
authors.affiliation: Plant Breeding Institute, University of Kiel Kiel, Germany.
authors.forename: Christian
authors.initials: C
authors.lastname: Jung
authors.affiliation: Institute of Computer Science, Martin Luther University Halle-WittenbergHalle, Germany; German Centre for Integrative Biodiversity Research (iDiv) Halle-Leipzig-JenaLeipzig, Germany.
authors.forename: Ivo
authors.initials: I
authors.lastname: Grosse
keywords: flowering time; mapping-by-sequencing; sugar beet; vernalization; winter beet