title: Novel perspectives on the role of the human microbiota in regenerative medicine and surgery.
abstract: Plastic surgery is transitioning from a fine craftsmanship to a regenerative science. In wound healing, the role of microorganisms is no longer considered to be just counteracting, but also promoting. Furthermore, host-microbe interactions are essential for numerous aspects of normal mammalian physiology, from metabolic activity to immune homeostasis. Each area of the human body hosts a unique microbial community, and the composition of microbiota is dependent on the host, age and the anatomical area, and it changes according to the characteristics of the microenvironment. Every squared centimeter of skin contains ~1 billion bacteria. The majority of microorganisms of the skin are commensal or temporary passing members. Skin flora mechanisms interacting or influencing the human physical skin barrier are not well defined. Resident skin bacteria provide the first line of defence against potentially dangerous pathogens and produce small molecules that influence their microbial neighbours. Furthermore, the microbiota activates and assists innate immunity and influences adaptive immunity. Various types of immune and non-immune cells contribute to wound healing. The proliferative phase of wound healing is inversely proportional to the extent of the post-traumatic inflammatory reaction. Topical bacterial lipopolysaccharide application markedly affects wound healing by accelerating the resolution of inflammation, increasing macrophage infiltration, enhancing collagen synthesis and altering the secretion of mediators involved in skin regeneration. Various studies have investigated the biological contents of thermal spring waters, and their anti-inflammatory and immune protective roles. In addition, the regenerative properties of thermal spring waters were analysed in an experimental animal wound model. The areas treated with thermal water healed faster than the areas treated with conventional dressings, and exhibited a collagen and elastic fiber network comparable with the normal skin. Thus, the microbial environment may be considered as a potential tool in regenerative medicine and surgery.
authors.affiliation: Department of Clinical-Surgical, Diagnostic and Pediatric Sciences, Plastic Surgery Unit, University of Pavia, Pavia, I-27100 Lombardy, Italy.
authors.forename: Tommaso
authors.initials: T
authors.lastname: Pellegatta
authors.affiliation: Department of Clinical-Surgical, Diagnostic and Pediatric Sciences, Plastic Surgery Unit, University of Pavia, Pavia, I-27100 Lombardy, Italy.
authors.forename: Marco
authors.initials: M
authors.lastname: Saler
authors.affiliation: Department of Clinical-Surgical, Diagnostic and Pediatric Sciences, Plastic Surgery Unit, University of Pavia, Pavia, I-27100 Lombardy, Italy.
authors.forename: Viola
authors.initials: V
authors.lastname: Bonfanti
authors.affiliation: Department of Clinical-Surgical, Diagnostic and Pediatric Sciences, Plastic Surgery Unit, University of Pavia, Pavia, I-27100 Lombardy, Italy; Plastic and Reconstructive Surgery Unit, Salvatore Maugeri Research and Care Institute, Pavia, I-27100 Lombardy, Italy.
authors.forename: Giovanni
authors.initials: G
authors.lastname: Nicoletti
authors.affiliation: Department of Clinical-Surgical, Diagnostic and Pediatric Sciences, Plastic Surgery Unit, University of Pavia, Pavia, I-27100 Lombardy, Italy; Plastic and Reconstructive Surgery Unit, Salvatore Maugeri Research and Care Institute, Pavia, I-27100 Lombardy, Italy.
authors.forename: Angela
authors.initials: A
authors.lastname: Faga
keywords: microbiota; regenerative medicine and surgery; spring water