title: The Influence of the Dietary Cu-Glycine Complex on the Histomorphology of Cancellous Bone, Articular Cartilage, and Growth Plate as well as Bone Mechanical and Geometric Parameters Is Dose Dependent.
abstract: Copper (Cu) is required for all basic biochemical and physiological processes. The objective of this study was to compare the effect of two different chemical forms (sulfates and glycinate chelates also below the recommended dose) of Cu administered to adult rats on the biomechanical and morphometric properties of femur. Male rats at the age of 12 weeks were used in the 12-week experiment. The control diet provided the required Cu level from sulfate (S-Cu), and the other diets were supplemented with Cu-glycine complex. The Cu-Gly-treatment, irrespective of its concentration, did not influence the bone mass and length. The Cu-Gly-treatment in 100 and 75% of daily demand increased mechanical endurance. The Cu-Gly-treatment (regardless of its concentration) increased the real bone volume in epiphysis and decreased the total thickness and zone I of the articular cartilage compared to the control group supplemented with S-Cu. The Cu-Gly-treatment enhanced the content of proteoglycans (except the OG50 group). Dietary Cu given to adult rats in the Cu-Gly complex covering the daily demand in 75% exerted a positive effect on bone metabolism and appeared to be the most effective among the investigated doses of the organic form.
authors.affiliation: Department of Animal Physiology, Faculty of Veterinary Medicine, University of Life Sciences in Lublin, Akademicka 12, 20-950, Lublin, Poland. ewaRST@interia.pl.
authors.forename: Ewa
authors.initials: E
authors.lastname: Tomaszewska
authors.affiliation: Department of Comparative Anatomy and Anthropology, Maria Curie-Skłodowska University, Akademicka 19, 20-033, Lublin, Poland.
authors.forename: Piotr
authors.initials: P
authors.lastname: Dobrowolski
authors.affiliation: Institute of Animal Nutrition and Bromatology, University of Life Sciences in Lublin, Akademicka 13, 20-950, Lublin, Poland.
authors.forename: Małgorzata
authors.initials: M
authors.lastname: Kwiecień
authors.affiliation: Institute of Animal Nutrition and Bromatology, University of Life Sciences in Lublin, Akademicka 13, 20-950, Lublin, Poland.
authors.forename: Anna
authors.initials: A
authors.lastname: Winiarska-Mieczan
authors.affiliation: Department of Animal Physiology, Faculty of Veterinary Medicine, University of Life Sciences in Lublin, Akademicka 12, 20-950, Lublin, Poland.
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Tomczyk
authors.affiliation: Department of Physics, Faculty of Production Engineering, University of Life Sciences in Lublin, Akademicka 13, 20-950, Lublin, Poland.
authors.forename: Siemowit
authors.initials: S
authors.lastname: Muszyński
mesh_terms: D000818:Animals; D000071556:Cancellous Bone / Q000378:metabolism*; D002358:Cartilage, Articular / Q000378:metabolism*; D003300:Copper / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D005998:Glycine / Q000494:pharmacology*; D006132:Growth Plate / Q000378:metabolism*; D008297:Male; D051381:Rats; D017208:Rats, Wistar
chemical_list: D003300:Copper; D005998:Glycine
keywords: Adult rat; Bone histomorphometry; Copper; Cu-Gly; Mechanical parameter