title: Spatial mapping of lichen specialized metabolites using LDI-MSI: chemical ecology issues for Ophioparma ventosa.
abstract: Imaging mass spectrometry techniques have become a powerful strategy to assess the spatial distribution of metabolites in biological systems. Based on auto-ionisability of lichen metabolites using LDI-MS, we herein image the distribution of major secondary metabolites (specialized metabolites) from the lichen Ophioparma ventosa by LDI-MSI (Mass Spectrometry Imaging). Such technologies offer tremendous opportunities to discuss the role of natural products through spatial mapping, their distribution patterns being consistent with previous chemical ecology reports. A special attention was dedicated to miriquidic acid, an unexpected molecule we first reported in Ophioparma ventosa. The analytical strategy presented herein offers new perspectives to access the sharp distribution of lichen metabolites from regular razor blade-sectioned slices.
authors.affiliation: Université Rennes 1, UMR CNRS 6226 PNSCM, 2 Avenue du Pr. L. Bernard, 35043 Cedex, France.|Institut d'Électronique et de Télécommunications de Rennes, Université Rennes 1, UMR CNRS 6164, 263 Avenue du Général Leclerc, 35042 Cedex, France.
authors.forename: Pierre
authors.initials: P
authors.lastname: Le Pogam
authors.affiliation: Université Rennes 1, UMR CNRS 6226 PNSCM, 2 Avenue du Pr. L. Bernard, 35043 Cedex, France.
authors.forename: Béatrice
authors.initials: B
authors.lastname: Legouin
authors.affiliation: INRA, UR 1268 Biopolymers Interactions Assemblies F-44316 Nantes, France.
authors.forename: Audrey
authors.initials: A
authors.lastname: Geairon
authors.affiliation: INRA, UR 1268 Biopolymers Interactions Assemblies F-44316 Nantes, France.
authors.forename: Hélène
authors.initials: H
authors.lastname: Rogniaux
authors.affiliation: Université Rennes 1, UMR CNRS 6226 PNSCM, 2 Avenue du Pr. L. Bernard, 35043 Cedex, France.
authors.forename: Françoise
authors.initials: F
authors.lastname: Lohézic-Le Dévéhat
authors.affiliation: Universitat Graz, Institut Karl Franzens, Holteigasse 6, A-8010 Graz, Austria.
authors.forename: Walter
authors.initials: W
authors.lastname: Obermayer
authors.affiliation: Université Rennes 1, UMR CNRS 6226 PNSCM, 2 Avenue du Pr. L. Bernard, 35043 Cedex, France.
authors.forename: Joël
authors.initials: J
authors.lastname: Boustie
authors.affiliation: Université Rennes 1, UMR CNRS 6226 PNSCM, 2 Avenue du Pr. L. Bernard, 35043 Cedex, France.|Université Toulouse 3 Paul Sabatier, UFR Pharmacie, 118 Route de Narbonne, 31062 Toulouse, France.
authors.forename: Anne-Cécile
authors.initials: AC
authors.lastname: Le Lamer
mesh_terms: D001203:Ascomycota / Q000378:metabolism*; D004463:Ecology; D008011:Lichens / Q000378:metabolism*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods