title: A Mouse Model of Multi-Drug ResistantStaphylococcus aureus-induced Ocular Disease.
abstract: Staphylococcus aureusinfection of the cornea is a significant threat to vision. The percentage of bacterial isolates resistant to antibiotics is increasing as is the percentage of infections caused by methicillin resistant isolates. There is a critical need for additional therapeutic approaches and their development will require the use of animal models to test efficacy. Two mouse models ofS. aureuskeratitis have been described but only quantified stromal keratitis (corneal clouding and perforation). We have extended these models using the methicillin resistantS. aureusUSA300 LAC strain and show that eyelid inflammation and swelling (blepharitis) and corneal neovascularization can be quantified. This expanded model should prove useful in assessing additional effects of antibacterial therapies and additional pathological mechanisms involved in bacterial ocular infection.
authors.affiliation: Amebagone, Inc.
authors.forename: Nicole M
authors.initials: NM
authors.lastname: Broekema
authors.affiliation: Department of Ophthalmology and Visual Sciences, School of Medicine and Public Health, University of Wisconsin-Madison, Wisconsin, USA.
authors.forename: Inna V
authors.initials: IV
authors.lastname: Larsen
authors.affiliation: Amebagone, Inc.
authors.forename: Erika S
authors.initials: ES
authors.lastname: Naruzawa
authors.affiliation: Amebagone, Inc.; Department of Bacteriology, University of Wisconsin-Madison, Wisconsin, USA.
authors.forename: Marcin
authors.initials: M
authors.lastname: Filutowicz
authors.affiliation: Department of Ophthalmology and Visual Sciences, School of Medicine and Public Health, University of Wisconsin-Madison, Wisconsin, USA.
authors.forename: Aaron W
authors.initials: AW
authors.lastname: Kolb
authors.affiliation: Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin-Madison, Wisconsin, USA.
authors.forename: Leandro B C
authors.initials: LB
authors.lastname: Teixeira
authors.affiliation: Department of Ophthalmology and Visual Sciences, School of Medicine and Public Health, University of Wisconsin-Madison, Wisconsin, USA; Department of Medical Microbiology and Immunology, School of Medicine and Public Health, University of Wisconsin-Madison, Wisconsin, USA; McPherson Eye Research Institute - University of Wisconsin-Madison, Wisconsin, USA.
authors.forename: Curtis R
authors.initials: CR
authors.lastname: Brandt
keywords: Bacterial keratitis; MRSA; Mouse model; Ocular disease; Staphylococcus aureus