title: Sasa quelpaertensis leaf extract regulates microbial dysbiosis by modulating the composition and diversity of the microbiota in dextran sulfate sodium-induced colitis mice.
abstract: BACKGROUND
  Inflammatory bowel diseases (IBD) are related to a dysfunction of the mucosal immune system and they result from complex interactions between genetics and environmental factors, including lifestyle, diet, and the gut microbiome. Therefore, the effect of Sasa quelpaertensis leaf extract (SQE) on gut microbiota in a dextran sulfate sodium (DSS)-induced colitis mouse model was investigated with pyrosequencing of fecal samples.
  
  
  METHODS
  Three groups of animals were examined: i) a control group, ii) a group that was received 2.5% DSS in their drinking water for 7 days, followed by 7 days of untreated water, and then another 7 days of 2.5% DSS in their drinking water, and iii) a group that was presupplemented with SQE (300 mg/kg body weight) by gavage for two weeks prior to the same DSS treatment schedule described in ii.
  
  
  RESULTS
  SQE supplementation alleviated disease activity scores and shortened colon length compared to the other two groups. In the DSS group, the proportion of Bacteroidetes increased, whereas that the proportion of Firmicutes was decreased compared to the control group. SQE supplementation recovered the proportions of Firmicutes and Bacteroidetes back to control levels. Moreover, the diversity of microbiota in the SQE supplementation group higher than that of the DSS group.
  
  
  CONCLUSIONS
  SQE was found to protect mice from microbial dysbiosis associated with colitis by modulating the microbial composition and diversity of the microbiota present. These results provide valuable insight into microbiota-food component interactions in IBD.
authors.affiliation: Department of Nutritional Science and Food Management, Ewha Womans University, Seoul, 03760, Republic of Korea.
authors.forename: Yiseul
authors.initials: Y
authors.lastname: Yeom
authors.affiliation: Department of Life Science, Hallym University, Chuncheon, Gangwon-do, 24252, Republic of Korea.
authors.forename: Bong-Soo
authors.initials: BS
authors.lastname: Kim
authors.affiliation: Department of Biology, Jeju National University, Jejusi, Jeju, 63243, Republic of Korea.
authors.forename: Se-Jae
authors.initials: SJ
authors.lastname: Kim
authors.affiliation: Department of Nutritional Science and Food Management, Ewha Womans University, Seoul, 03760, Republic of Korea. yuri.kim@ewha.ac.kr.
authors.forename: Yuri
authors.initials: Y
authors.lastname: Kim
mesh_terms: D000818:Animals; D003092:Colitis / Q000139:chemically induced / Q000188:drug therapy* / Q000382:microbiology / Q000473:pathology; D003106:Colon / Q000187:drug effects / Q000473:pathology; D004269:DNA, Bacterial; D016264:Dextran Sulfate; D064806:Dysbiosis / Q000188:drug therapy* / Q000382:microbiology; D005243:Feces / Q000382:microbiology; D000069196:Gastrointestinal Microbiome / Q000187:drug effects*; D059014:High-Throughput Nucleotide Sequencing; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D010936:Plant Extracts / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D031787:Sasa / Q000737:chemistry*
chemical_list: D004269:DNA, Bacterial; D010936:Plant Extracts; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D016264:Dextran Sulfate
keywords: Dextran sulfate sodium; Gut microbiota; Inflammatory bowel disease; Sasa quelpaertensis leaf extract