title: Non-conventional expression systems for the production of vaccine proteins and immunotherapeutic molecules.
abstract: The increasing demand for recombinant vaccine antigens or immunotherapeutic molecules calls into question the universality of current protein expression systems. Vaccine production can require relatively low amounts of expressed materials, but represents an extremely diverse category consisting of different target antigens with marked structural differences. In contrast, monoclonal antibodies, by definition share key molecular characteristics and require a production system capable of very large outputs, which drives the quest for highly efficient and cost-effective systems. In discussing expression systems, the primary assumption is that a universal production platform for vaccines and immunotherapeutics will unlikely exist. This review provides an overview of the evolution of traditional expression systems, including mammalian cells, yeast and E.coli, but also alternative systems such as other bacteria than E. coli, transgenic animals, insect cells, plants and microalgae, Tetrahymena thermophila, Leishmania tarentolae, filamentous fungi, cell free systems, and the incorporation of non-natural amino acids.
authors.affiliation: a Research and Development, Sanofi Pasteur , Marcy L'Etoile , France.
authors.forename: Isabelle
authors.initials: I
authors.lastname: Legastelois
authors.affiliation: a Research and Development, Sanofi Pasteur , Marcy L'Etoile , France.
authors.forename: Sophie
authors.initials: S
authors.lastname: Buffin
authors.affiliation: a Research and Development, Sanofi Pasteur , Marcy L'Etoile , France.
authors.forename: Isabelle
authors.initials: I
authors.lastname: Peubez
authors.affiliation: b Technology Research Institute Bioaster , Lyon , France.
authors.forename: Charlotte
authors.initials: C
authors.lastname: Mignon
authors.affiliation: b Technology Research Institute Bioaster , Lyon , France.
authors.forename: Régis
authors.initials: R
authors.lastname: Sodoyer
authors.affiliation: b Technology Research Institute Bioaster , Lyon , France.
authors.forename: Bettina
authors.initials: B
authors.lastname: Werle
mesh_terms: D001709:Biotechnology / Q000379:methods; D015870:Gene Expression; D005822:Genetic Vectors; D007155:Immunologic Factors / Q000302:isolation & purification* / Q000378:metabolism*; D008967:Molecular Biology / Q000379:methods; D011994:Recombinant Proteins / Q000302:isolation & purification* / Q000378:metabolism*; D013678:Technology, Pharmaceutical / Q000379:methods; D014612:Vaccines / Q000302:isolation & purification* / Q000378:metabolism*
chemical_list: D007155:Immunologic Factors; D011994:Recombinant Proteins; D014612:Vaccines
keywords: Non-conventional expression systems; immunotherapeutics; monoclonal antibodies; universal production platform; vaccines