title: Mutations Associated with Decreased Susceptibility to Seven Antimicrobial Families in Field and Laboratory-Derived Mycoplasma bovis Strains.
abstract: The molecular mechanisms of resistance to fluoroquinolones, tetracyclines, an aminocyclitol, macrolides, a lincosamide, a phenicol, and pleuromutilins were investigated in Mycoplasma bovis For the identification of mutations responsible for the high MICs of certain antibiotics, whole-genome sequencing of 35 M. bovis field isolates and 36 laboratory-derived antibiotic-resistant mutants was performed. In vitro resistant mutants were selected by serial passages of M. bovis in broth medium containing subinhibitory concentrations of the antibiotics. Mutations associated with high fluoroquinolones MICs were found at positions 244 to 260 and at positions 232 to 250 (according to Escherichia coli numbering) of the quinolone resistance-determining regions of the gyrA and parC genes, respectively. Alterations related to elevated tetracycline MICs were described at positions 962 to 967, 1058, 1195, 1196, and 1199 of genes encoding the 16S rRNA and forming the primary tetracycline binding site. Single transversion at position 1192 of the rrs1 gene resulted in a spectinomycin MIC of 256 μg/ml. Mutations responsible for high macrolide, lincomycin, florfenicol, and pleuromutilin antibiotic MICs were identified in genes encoding 23S rRNA. Understanding antibiotic resistance mechanisms is an important tool for future developments of genetic-based diagnostic assays for the rapid detection of resistant M. bovis strains.
authors.affiliation: Institute for Veterinary Medical Research, Centre for Agricultural Research, Hungarian Academy of Sciences, Budapest, Hungary.
authors.forename: Kinga M
authors.initials: KM
authors.lastname: Sulyok
authors.affiliation: Institute for Veterinary Medical Research, Centre for Agricultural Research, Hungarian Academy of Sciences, Budapest, Hungary.
authors.forename: Zsuzsa
authors.initials: Z
authors.lastname: Kreizinger
authors.affiliation: Institute for Veterinary Medical Research, Centre for Agricultural Research, Hungarian Academy of Sciences, Budapest, Hungary.
authors.forename: Enikő
authors.initials: E
authors.lastname: Wehmann
authors.affiliation: Division of Avian and Aquatic Diseases, Kimron Veterinary Institute, Bet Dagan, Israel.
authors.forename: Inna
authors.initials: I
authors.lastname: Lysnyansky
authors.affiliation: Institute for Veterinary Medical Research, Centre for Agricultural Research, Hungarian Academy of Sciences, Budapest, Hungary.
authors.forename: Krisztián
authors.initials: K
authors.lastname: Bányai
authors.affiliation: Institute for Veterinary Medical Research, Centre for Agricultural Research, Hungarian Academy of Sciences, Budapest, Hungary.
authors.forename: Szilvia
authors.initials: S
authors.lastname: Marton
authors.affiliation: Department of Pharmacology and Toxicology, University of Veterinary Medicine, Budapest, Hungary.
authors.forename: Ákos
authors.initials: Á
authors.lastname: Jerzsele
authors.affiliation: Veterinary Diagnostic Directorate, National Food Chain Safety Office, Budapest, Hungary.
authors.forename: Zsuzsanna
authors.initials: Z
authors.lastname: Rónai
authors.affiliation: Veterinary Diagnostic Directorate, National Food Chain Safety Office, Budapest, Hungary.
authors.forename: Ibolya
authors.initials: I
authors.lastname: Turcsányi
authors.affiliation: Department of Microbiology and Infectious Diseases, University of Veterinary Medicine, Budapest, Hungary.
authors.forename: László
authors.initials: L
authors.lastname: Makrai
authors.affiliation: Veterinary Diagnostic Directorate, National Food Chain Safety Office, Budapest, Hungary.
authors.forename: Szilárd
authors.initials: S
authors.lastname: Jánosi
authors.affiliation: Institute for Veterinary Medical Research, Centre for Agricultural Research, Hungarian Academy of Sciences, Budapest, Hungary.
authors.forename: Sára Ágnes
authors.initials: SÁ
authors.lastname: Nagy
authors.affiliation: Institute for Veterinary Medical Research, Centre for Agricultural Research, Hungarian Academy of Sciences, Budapest, Hungary m.gyuranecz@gmail.com.
authors.forename: Miklós
authors.initials: M
authors.lastname: Gyuranecz
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D000890:Anti-Infective Agents / Q000494:pharmacology*; D002417:Cattle; D004352:Drug Resistance, Microbial / Q000235:genetics; D024841:Fluoroquinolones / Q000494:pharmacology; D008826:Microbial Sensitivity Tests; D009154:Mutation / Q000235:genetics; D045682:Mycoplasma bovis / Q000166:cytology* / Q000235:genetics*; D012336:RNA, Ribosomal, 16S / Q000235:genetics
chemical_list: D000900:Anti-Bacterial Agents; D000890:Anti-Infective Agents; D024841:Fluoroquinolones; D012336:RNA, Ribosomal, 16S
keywords: Mycoplasma bovis; antibiotic resistance; cattle