title: Quantitative label-free mass spectrometry analysis of formalin-fixed, paraffin-embedded tissue representing the invasive cutaneous malignant melanoma proteome.
abstract: Understanding the events at a protein level that govern the progression from melanomain situto invasive melanoma are important areas of current research to be developed. Recent advances in the analysis of formalin-fixed, paraffin-embedded tissue by proteomics, particularly using the filter-aided sample preparation protocol, has opened up the possibility of studying vast archives of clinical material and associated medical records. In the present study, quantitative protein profiling was performed using tandem mass spectrometry, and the proteome differences between melanomain situand invasive melanoma were compared. Biological pathway analyses revealed several signalling pathways differing between melanomain situand invasive melanoma, including metabolic pathways and the phosphoinositide 3-kinase-Akt signalling pathway. Selected proteins of interest (14-3-3ε and fatty acid synthase) were subsequently investigated using immunohistochemical analysis of tissue microarrays. Identifying the key proteins that play significant roles in the establishment of a more invasive phenotype in melanoma may ultimately aid diagnosis and treatment decisions.
authors.affiliation: Department of Biology, Maynooth University, Maynooth, Co. Kildare, Republic of Ireland.
authors.forename: Paul
authors.initials: P
authors.lastname: Dowling
authors.affiliation: National Institute for Cellular Biotechnology, Dublin City University, Dublin 9, Republic of Ireland; Department of Dermatology, St. James's Hospital, Trinity College Dublin, Dublin 8, Republic of Ireland.
authors.forename: Benvon
authors.initials: B
authors.lastname: Moran
authors.affiliation: National Institute for Cellular Biotechnology, Dublin City University, Dublin 9, Republic of Ireland.
authors.forename: Edel
authors.initials: E
authors.lastname: McAuley
authors.affiliation: National Institute for Cellular Biotechnology, Dublin City University, Dublin 9, Republic of Ireland.
authors.forename: Paula
authors.initials: P
authors.lastname: Meleady
authors.affiliation: National Institute for Cellular Biotechnology, Dublin City University, Dublin 9, Republic of Ireland.
authors.forename: Michael
authors.initials: M
authors.lastname: Henry
authors.affiliation: National Institute for Cellular Biotechnology, Dublin City University, Dublin 9, Republic of Ireland.
authors.forename: Martin
authors.initials: M
authors.lastname: Clynes
authors.affiliation: Department of Dermatology, St. James's Hospital, Trinity College Dublin, Dublin 8, Republic of Ireland.
authors.forename: Mairin
authors.initials: M
authors.lastname: McMenamin
authors.affiliation: Department of Dermatology, St. James's Hospital, Trinity College Dublin, Dublin 8, Republic of Ireland.
authors.forename: Niamh
authors.initials: N
authors.lastname: Leonard
authors.affiliation: Department of Dermatology, St. James's Hospital, Trinity College Dublin, Dublin 8, Republic of Ireland.
authors.forename: Mary
authors.initials: M
authors.lastname: Monks
authors.affiliation: Department of Dermatology, St. James's Hospital, Trinity College Dublin, Dublin 8, Republic of Ireland.
authors.forename: Bairbre
authors.initials: B
authors.lastname: Wynne
authors.affiliation: Department of Dermatology, St. James's Hospital, Trinity College Dublin, Dublin 8, Republic of Ireland.
authors.forename: Patrick
authors.initials: P
authors.lastname: Ormond
authors.affiliation: National Institute for Cellular Biotechnology, Dublin City University, Dublin 9, Republic of Ireland.
authors.forename: Annemarie
authors.initials: A
authors.lastname: Larkin
keywords: 14-3-3ε; FASN; FFPE; mass spectrometry; melanoma; proteomics