title: Nitrobacter sp.extract mediated biosynthesis of Ag2O NPs with excellent antioxidant and antibacterial potential for biomedical application.
abstract: In this study, extracellular extract of plant growth promoting bacterium, Nitrobacter sp. is used for the bioconversion of AgNO3(silver nitrate) into Ag2O (silver oxide nanoparticles). It is an easy, ecofriendly and single step method for Ag2O NPs synthesis. The bio-synthesized nanoparticles were characterized using different techniques. UV-Vis results showed the maximum absorbance around 450 nm. XRD result shows the particles to have faced centered cubic (fcc) crystalline nature. FTIR analysis reveals the functional groups that are involved in bioconversion such as C-N, N-H and C=O. Energy-dispersive X-ray spectroscopy (EDAX) spectrum confirms that the prepared nanoparticle is Ag2O NPs. Particle size distribution result reveals that the average particle size is around 40 nm. The synthesized Ag2O NPs found to be almost spherical in shape. Biosynthesized Ag2O NPs possess good antibacterial activity against selected Gram positive and Gram negative bacterial strains namely Salmonella typhimurium, Staphylococcus aureus, Escherichia coli and Klebsiella pneumoniae when compared to standard antibiotic. In addition, Ag2O NPs exhibits excellent free radical scavenging activity with respect to dosage. Thus, this study is a new approach to use soil bacterial extract for the production of Ag2O NPs for biomedical application.
authors.affiliation: Department of Biotechnology, K. S. Rangasamy College of Arts and Science, Tiruchengode 637215, Tamil Nadu, India. karunakarang5@gmail.com.
authors.forename: Gopalu
authors.initials: G
authors.lastname: Karunakaran
authors.affiliation: Department of Bio-chemistry/Bio-technology/Bio-informatics, Avinashilingam Institute for Home Science and Higher Education for Women, Mettupalayam Road, Bharathi Park Road, Coimbatore 641 043, India.
authors.forename: Matheswaran
authors.initials: M
authors.lastname: Jagathambal
authors.affiliation: G. R. Derzhavin Tambov State University, 33, Internatsionalnaya Street, Tambov 392000, Russia.
authors.forename: Alexander
authors.initials: A
authors.lastname: Gusev
authors.affiliation: Department of Functional Nanosystems and High-Temperature Materials, National University of Science and Technology 'MISiS', Leninskiy Pr. 4, Moscow 119049, Russia.
authors.forename: Nguyen Van
authors.initials: NV
authors.lastname: Minh
authors.affiliation: Department of Functional Nanosystems and High-Temperature Materials, National University of Science and Technology 'MISiS', Leninskiy Pr. 4, Moscow 119049, Russia.
authors.forename: Evgeny
authors.initials: E
authors.lastname: Kolesnikov
authors.affiliation: Department of Functional Nanosystems and High-Temperature Materials, National University of Science and Technology 'MISiS', Leninskiy Pr. 4, Moscow 119049, Russia.
authors.forename: Arup Ratan
authors.initials: AR
authors.lastname: Mandal
authors.affiliation: Department of Functional Nanosystems and High-Temperature Materials, National University of Science and Technology 'MISiS', Leninskiy Pr. 4, Moscow 119049, Russia.
authors.forename: Denis
authors.initials: D
authors.lastname: Kuznetsov
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D000975:Antioxidants / Q000494:pharmacology*; D053768:Metal Nanoparticles; D008826:Microbial Sensitivity Tests; D009576:Nitrobacter / Q000737:chemistry*; D010087:Oxides; D018030:Silver Compounds; D012835:Silver Nitrate
chemical_list: D000900:Anti-Bacterial Agents; D000975:Antioxidants; D010087:Oxides; D018030:Silver Compounds; C040225:disilver oxide; D012835:Silver Nitrate