title: Rapid mesoscale multiphoton microscopy of human skin.
abstract: We present a multiphoton microscope designed for mesoscale imaging of human skin. The system is based on two-photon excited fluorescence and second-harmonic generation, and images areas of ~0.8x0.8 mm2at speeds of 0.8 fps (800x800 pixels; 12 frame averages) for high signal-to-noise ratio, with lateral and axial resolutions of 0.5µm and 3.3µm, respectively. The main novelty of this instrument is the design of the scan head, which includes a fast galvanometric scanner, optimized relay optics, a beam expander and high NA objective lens. Computed aberrations in focus are below the Marechal criterion of 0.07λ rms for diffraction-limited performance. We demonstrate the practical utility of this microscope byex-vivoimaging of wide areas in normal human skin.
authors.affiliation: University of California, Irvine, Beckman Laser Institute, Laser Microbeam and Medical Program, Irvine, CA 92612, USA.
authors.forename: Mihaela
authors.initials: M
authors.lastname: Balu
authors.affiliation: Current Affiliation: Department of Chemistry School of Science, University of Tokyo, Tokyo, Japan; Department of Chemistry, University of California, Irvine, CA 92697, USA.
authors.forename: Hideharu
authors.initials: H
authors.lastname: Mikami
authors.affiliation: University of California, Irvine, Beckman Laser Institute, Laser Microbeam and Medical Program, Irvine, CA 92612, USA.
authors.forename: Jue
authors.initials: J
authors.lastname: Hou
authors.affiliation: University of California, Irvine, Beckman Laser Institute, Laser Microbeam and Medical Program, Irvine, CA 92612, USA; Department of Chemistry, University of California, Irvine, CA 92697, USA.
authors.forename: Eric O
authors.initials: EO
authors.lastname: Potma
authors.affiliation: University of California, Irvine, Beckman Laser Institute, Laser Microbeam and Medical Program, Irvine, CA 92612, USA.
authors.forename: Bruce J
authors.initials: BJ
authors.lastname: Tromberg
keywords: (170.2520) Fluorescence microscopy; (180.0180) Microscopy; (180.4315) Nonlinear microscopy