title: Extraction of Volatile Flavor Compounds From Tobacco Leaf Through a Low-Density Polyethylene Membrane.
abstract: A low-density polyethylene (LDPE) membrane pouch method was developed to extract volatile flavor compounds from tobacco leaf. Tobacco leaf suspended in water was enclosed in a pouch prepared from a LDPE membrane of specific gravity 0.92 g/cm3 and 0.03 mm thickness and then extracted with diethyl ether. In comparison with direct solvent extraction, LDPE membrane excluded larger and higher boiling point compounds which could contaminate a gas chromatograph inlet and damage a column. Whilst being more convenient than a reduced-pressure steam distillation, it could extract volatile flavor compounds of wide range of molecular weight and polarity. Repeatabilities in the extracted amounts were ranged from 0.38% of 2.3-bipyridyl to 26% of β-ionone, and average value of 39 compounds was 5.9%.
authors.affiliation: Tobacco Science Research Center, Japan Tobacco Inc., 6-2, Umegaoka, Aoba-ku, Yokohama227-8512, Japan.
authors.forename: Michinori
authors.initials: M
authors.lastname: Yokoi
authors.affiliation: Food Process Engineering, Division of Food Science and Technology, Bioresource and Bioenvironmental Science, Kyushu University, 6-10-1, Hakozaki, Higashi-ku, Fukuoka812-8581, Japan.
authors.forename: Mitsuya
authors.initials: M
authors.lastname: Shimoda
mesh_terms: D008401:Gas Chromatography-Mass Spectrometry; D008567:Membranes, Artificial; D010936:Plant Extracts / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification*; D018515:Plant Leaves / Q000737:chemistry*; D020959:Polyethylene / Q000737:chemistry*; D015203:Reproducibility of Results; D014026:Nicotiana / Q000737:chemistry*; D055549:Volatile Organic Compounds / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification*
chemical_list: D008567:Membranes, Artificial; D010936:Plant Extracts; D055549:Volatile Organic Compounds; D020959:Polyethylene