title: SNP genotyping by DNA photoligation: application to SNP detection of genes from food crops.
abstract: We describe a simple and inexpensive single-nucleotide polymorphism (SNP) typing method, using DNA photoligation with 5-carboxyvinyl-2'-deoxyuridine and two fluorophores. This SNP-typing method facilitates qualitative determination of genes from indica and japonica rice, and showed a high degree of single nucleotide specificity up to 10 000. This method can be used in the SNP typing of actual genomic DNA samples from food crops.
authors.affiliation: School of Materials Science, Japan Advanced Institute of Science and Technology, 1-1 Asahidai, Nomi, Ishikawa 923-1292, Japan; Innovation Plaza Ishikawa, Japan Science and Technology Agency, 2-13 Asahidai, Nomi, Ishikawa 923-1211, Japan.
authors.forename: Yoshinaga
authors.initials: Y
authors.lastname: Yoshimura
authors.affiliation: School of Materials Science, Japan Advanced Institute of Science and Technology, 1-1 Asahidai, Nomi, Ishikawa 923-1292, Japan.
authors.forename: Tomoko
authors.initials: T
authors.lastname: Ohtake
authors.affiliation: School of Materials Science, Japan Advanced Institute of Science and Technology, 1-1 Asahidai, Nomi, Ishikawa 923-1292, Japan.
authors.forename: Hajime
authors.initials: H
authors.lastname: Okada
authors.affiliation: Innovation Plaza Ishikawa, Japan Science and Technology Agency, 2-13 Asahidai, Nomi, Ishikawa 923-1211, Japan.
authors.forename: Takehiro
authors.initials: T
authors.lastname: Ami
authors.affiliation: Faculty of Bioresources and Environmental Sciences, Ishikawa Prefectural University, 1-308 Suematsu, Nonoichi, Ishikawa 921-8836, Japan.
authors.forename: Tadashi
authors.initials: T
authors.lastname: Tsukaguchi
authors.affiliation: School of Materials Science, Japan Advanced Institute of Science and Technology, 1-1 Asahidai, Nomi, Ishikawa 923-1292, Japan; Innovation Plaza Ishikawa, Japan Science and Technology Agency, 2-13 Asahidai, Nomi, Ishikawa 923-1211, Japan.
authors.forename: Kenzo
authors.initials: K
authors.lastname: Fujimoto
keywords: DNA recognition; biosensors; food crops; ligation reactions; photochemistry