title: Evaluation of anti-proliferative and anti-inflammatory activities of Pelagia noctiluca venom in Lipopolysaccharide/Interferon-γ stimulated RAW264.7 macrophages.
abstract: Components of Pelagia noctiluca (P. noctiluca) venom were evaluated for their anticancer and nitric Oxide (NO) inhibition activities. Three fractions, out of four, obtained by gel filtration on Sephadex G75 of P. noctiluca venom revealed an important selective anti-proliferative activity on several cell lines such as human bladder carcinoma (RT112), human glioblastoma (U87), and human myelogenous leukemia (K562) but not on mitogen-stimulated peripheral blood mononuclear cells. Interestingly, P. noctiluca components showed an important dose-dependent anti-inflammatory activity, through inhibition of NO production via transcriptional regulation of Inducible NO Synthase (iNOS), in IFN-γ/LPS stimulated RAW 264.7 macrophages. These data strongly suggest that P. noctiluca venom could be used as a natural inhibitor of cancer cell lines and a potent anti-inflammatory agent for the treatment of anti-inflammatory diseases.
authors.affiliation: College of Dentistry, Taibah University, PO Box 30034, Madina 41477, Saudi Arabia; Faculty of Dental Medicine of Monastir, Laboratory for Research on Biologically Compatible Compounds, Rue Avicenne, 5019 Monastir, Tunisia. Electronic address: yosraayed82@gmail.com.
authors.forename: Yosra
authors.initials: Y
authors.lastname: Ayed
authors.affiliation: Institut Pasteur de Tunis, Laboratory of Transmission, Control and Immunobiolgy of Infections, LR11IPT02 (LTCII), Tunis-Belvédère 1002, Tunisia; University of Tunis El Manar, Tunis, 1068, Tunisia.
authors.forename: Rabiaa Manel
authors.initials: RM
authors.lastname: Sghaier
authors.affiliation: Institut Pasteur de Tunis, Laboratory of Transmission, Control and Immunobiolgy of Infections, LR11IPT02 (LTCII), Tunis-Belvédère 1002, Tunisia.
authors.forename: Dhafer
authors.initials: D
authors.lastname: Laouini
authors.affiliation: Faculty of Dental Medicine of Monastir, Laboratory for Research on Biologically Compatible Compounds, Rue Avicenne, 5019 Monastir, Tunisia; University of Jendouba, AlFaeiz, Street Jamil Boutheina Jendouba 8100, Tunisia.
authors.forename: Hassen
authors.initials: H
authors.lastname: Bacha
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000302:isolation & purification / Q000494:pharmacology*; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects* / Q000502:physiology; D002470:Cell Survival / Q000187:drug effects / Q000502:physiology; D003064:Cnidarian Venoms / Q000302:isolation & purification / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004353:Drug Evaluation, Preclinical / Q000379:methods; D006801:Humans; D007371:Interferon-gamma / Q000633:toxicity*; D020014:K562 Cells; D007963:Leukocytes, Mononuclear / Q000187:drug effects / Q000378:metabolism; D008070:Lipopolysaccharides / Q000633:toxicity*; D008264:Macrophages / Q000187:drug effects* / Q000378:metabolism; D051379:Mice; D052247:Nitric Oxide Synthase Type II / Q000037:antagonists & inhibitors / Q000378:metabolism
chemical_list: D000893:Anti-Inflammatory Agents; D003064:Cnidarian Venoms; D008070:Lipopolysaccharides; D007371:Interferon-gamma; D052247:Nitric Oxide Synthase Type II
keywords: Anti-inflammatory activity; Cell proliferation; MTT assay; Nitric oxide; Pelagia noctiluca; Venom