title: Isolation and identification of culturable halophilic bacteria with producing hydrolytic enzyme from Incheh Broun hypersaline wetland in Iran.
abstract: Incheh Broun hypersaline wetland is located near the border of Turkmenistan in thenorth of Iran. This wetland is notable because of salinity (280g/l) and alteration in pH range (2.8 to 6.8). Eastern part of wetland is affected by wastewater of iodine extraction factory.  Samples were taken from soil, water and salt. Totally, 400 bacterial strains were isolated of which 194 strains were Gram-positive bacilli, 184 strains were Gram-negative rod and 22 strains were Gram-positive cocci. According to phylogenetic analysis of 16S rRNA, selected strains were placed in three taxonomic phyla including Firmicutes, Actinobacteria and Gammaproteobacteria. Optimum growth was evaluated for salt and 22 strains were found to be moderate halophile and 33 strains were halotolerant. Production of lipase, amylase, gelatinase and protease was examined. Gram-positive bacilli were the main producers of hydrolytic enzymes. Gelatinase and protease were the most frequent enzymes. Gram-positive cocci were the main producers of lipase but they didn't produce amylase.
authors.affiliation: Department of Biology, Science and Research Branch,Islamic Azad University, Tehran, Iran.
authors.forename: P
authors.initials: P
authors.lastname: Zarparvar
authors.affiliation: Extremophiles Laboratory, Department of Microbiology, School of Biology and Center of excellence in Phylogeny of Living Organisms, College of Science, University of Tehran, Iran. amoozegar@ut.ac.ir.
authors.forename: M A
authors.initials: MA
authors.lastname: Amoozegar
authors.affiliation: Applied Virology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran. h.babavalian@yahoo.com.
authors.forename: H
authors.initials: H
authors.lastname: Babavalian
authors.affiliation: Department of Biology,North Branch, Islamic Azad University, Tehran, Iran.
authors.forename: M
authors.initials: M
authors.lastname: Reza Fallahian
authors.affiliation: Applied Biotechnology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran.
authors.forename: H
authors.initials: H
authors.lastname: Tebyanian
authors.affiliation: Applied Biotechnology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran.
authors.forename: F
authors.initials: F
authors.lastname: Shakeri
mesh_terms: D001419:Bacteria / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D004269:DNA, Bacterial / Q000302:isolation & purification / Q000378:metabolism; D006090:Gram-Negative Bacteria / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D006094:Gram-Positive Bacteria / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D006863:Hydrogen-Ion Concentration; D007492:Iran; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D054712:Salinity; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D014865:Waste Disposal, Fluid; D053833:Wetlands
chemical_list: D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S