title: Blastococcus colisei sp. nov, isolated from an archaeological amphitheatre.
abstract: The taxonomic position of an actinobacterial isolate, designated strain BMG 822T, isolated from limestone from the Amphitheater of El Jem (Coliseum Thysdrus), Tunisia, was established using a polyphasic approach. Strain BMG 822Twas found to grow well at 30 °C and pH 6.5-8.0, and to be coral-coloured, Gram-positive, catalase and oxidase negative. Whole cell hydrolysates contained meso-diaminopimelic acid as the diagnostic diamino acid, glucose, galactose and ribose. The phospholipids detected were diphosphatidylglycerol, phosphatidylcholine, phosphatidylinositol, phosphatidylethanolamine, hydroxy-phosphatidylethanolamine, an unidentified glycophospholipid and six unidentified phospholipids. MK-9(H4) was found to be the predominant menaquinone, followed by MK-9(H2) and MK-9. The major cellular fatty acids were identified as iso-C16:0, C18:1ω9c, C17:1ω8c and iso-H-C16:1. The G+C content of the DNA (73.2%) is typical of the genus. High degrees of 16S rRNA gene sequence similarity were found with the type strains of the genus Blastococcus (97.1-98.3%) followed by the type strains of Modestobacter (96.8-97.8%). Based on the above data and the phenotypic differences from the type strains of Blastococcus species, it is proposed that the isolate BMG 822T(=DSM 46837T=CECT 8823T) should be classified as the type strain of a novel species, Blastococcus colisei sp. nov.
authors.affiliation: Laboratoire Microorganismes et Biomolécules Actives, Université de Tunis El Manar (FST) & Université de Carthage (INSAT), 2092, Tunis, Tunisia.
authors.forename: Karima
authors.initials: K
authors.lastname: Hezbri
authors.affiliation: School of Biology, Newcastle University, Ridley Building 2, Newcastle upon Tyne, NE1 7RU, UK.
authors.forename: Imen
authors.initials: I
authors.lastname: Nouioui
authors.affiliation: Central Facility for Microscopy, HZI - Helmholtz Centre for Infection Research, Inhoffenstraße 7, 38124, Brunswick, Germany.
authors.forename: Manfred
authors.initials: M
authors.lastname: Rohde
authors.affiliation: Leibniz Institute DSMZ - German Collection of Microorganisms and Cell Cultures, Inhoffenstraße 7B, 38124, Brunswick, Germany.
authors.forename: Peter
authors.initials: P
authors.lastname: Schumann
authors.affiliation: Laboratoire Microorganismes et Biomolécules Actives, Université de Tunis El Manar (FST) & Université de Carthage (INSAT), 2092, Tunis, Tunisia.
authors.forename: Maher
authors.initials: M
authors.lastname: Gtari
authors.affiliation: School of Biology, Newcastle University, Ridley Building 2, Newcastle upon Tyne, NE1 7RU, UK.
authors.forename: Hans-Peter
authors.initials: HP
authors.lastname: Klenk
authors.affiliation: School of Biology, Newcastle University, Ridley Building 2, Newcastle upon Tyne, NE1 7RU, UK. calasanz@newcastle.ac.uk.|Leibniz Institute DSMZ - German Collection of Microorganisms and Cell Cultures, Inhoffenstraße 7B, 38124, Brunswick, Germany. calasanz@newcastle.ac.uk.
authors.forename: Maria Del Carmen
authors.initials: MD
authors.lastname: Montero-Calasanz
authors.affiliation: Laboratoire Microorganismes et Biomolécules Actives, Université de Tunis El Manar (FST) & Université de Carthage (INSAT), 2092, Tunis, Tunisia. feten.ghodhbane@fst.rnu.tn.
authors.forename: Faten
authors.initials: F
authors.lastname: Ghodhbane-Gtari
mesh_terms: D000192:Actinomycetales / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D002473:Cell Wall / Q000737:chemistry; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D010641:Phenotype; D010743:Phospholipids / Q000032:analysis; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012965:Sodium Chloride; D012988:Soil Microbiology; D013045:Species Specificity; D014416:Tunisia; D024482:Vitamin K 2 / Q000032:analysis
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; D012965:Sodium Chloride
keywords: Geodermatophilaceae; Novel species; Polyphasic approach