title: Expression of long non-coding RNA-HOTAIR in oral squamous cell carcinoma Tca8113 cells and its associated biological behavior.
abstract: As a long noncoding RNA, HOX transcript antisense intergenic RNA (HOTAIR) is highly expressed in many types of tumors. However, its expression and function in oral squamous cell carcinoma (OSCC) cells and tissues remains largely unknown. We herein studied the biological functions of HOTAIR in OSCC Tca8113 cells. Real-time quantitative PCR showed that HOTAIR, p21 and p53 mRNA expressions in doxorubicin (DOX)-treated or γ-ray-irradiated Tca8113 cells were up-regulated. Knockdown of p53 expression inhibited DOX-induced HOTAIR up-regulation, suggesting that DNA damage-induced HOTAIR expression may be associated with p53. Transfection and CCK-8 assays showed that compared with the control group, overexpression of HOTAIR promoted the proliferation of Tca8113 cells, while interfering with its expression played an opposite role. Flow cytometry exhibited that HOTAIR overexpression decreased the rate of DOX-induced apoptosis. When HOTAIR expression was inhibited by siRNA, the proportions of cells in G2/M and S phases increased and decreased respectively. Meanwhile, the rate of DOX-induced apoptosis rose. DNA damage-induced HOTAIR expression facilitated the proliferation of Tca8113 cells and decreased their apoptosis. However, whether the up-regulation depends on p53 still needs in-depth studies.
authors.affiliation: Department of Stomatology, Chinese PLA General Hospital Beijing 100853, China.
authors.forename: Huawei
authors.initials: H
authors.lastname: Liu
authors.affiliation: Department of Military Special Clinic Surgery, 451th Hospital of The People's Liberation Army Xi'an 710049, China.
authors.forename: Zhiyong
authors.initials: Z
authors.lastname: Li
authors.affiliation: Department of Stomatology, Chinese PLA General Hospital Beijing 100853, China.
authors.forename: Chao
authors.initials: C
authors.lastname: Wang
authors.affiliation: Department of Stomatology, Chinese PLA General Hospital Beijing 100853, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Feng
authors.affiliation: Department of Stomatology, Chinese PLA General Hospital Beijing 100853, China.
authors.forename: Haitao
authors.initials: H
authors.lastname: Huang
authors.affiliation: Department of Military Special Clinic Surgery, 451th Hospital of The People's Liberation Army Xi'an 710049, China.
authors.forename: Changkui
authors.initials: C
authors.lastname: Liu
authors.affiliation: Department of Stomatology, Ankang Hospital of Traditional Chinese Medicine Ankang 725000, China.
authors.forename: Fengxia
authors.initials: F
authors.lastname: Li
keywords: DNA damage; HOX transcript antisense intergenic RNA; Long noncoding RNA; oral squamous cell carcinoma