title: Chryseobacterium lineare sp. nov., isolated from a limpid stream.
abstract: A Gram-stain-negative, aerobic, non-motile, rod-shaped, yellow-pigmented bacterial strain, XC0022T, isolated from freshwater of a limpid stream in Zhejiang, China, was studied using a polyphasic approach. The phylogenetic analysis based on 16S rRNA gene sequences clearly showed an allocation to the genus Chryseobacterium with the highest sequence similarities of 98.0 % to Chryseobacterium taeanense PHA3-4T, 97.2 % to Chryseobacterium taihuense THMBM1T, 97.1 % to Chryseobacterium rigui CJ16T and 97.1 % to Chryseobacteriumprofundimaris DY46T. 16S rRNA gene sequence similarities to all other species of the genus Chryseobacterium were below 97.0 % (92.3-96.8 %). DNA-DNA hybridization results showed that strain XC0022T was 55.3 %, 49.8 % and 31.1 % related to C. taeanense DSM 17071T, Chryseobacteriumtaichungense DSM 17453T and Chryseobacteriumgleum JCM 2410T, respectively. The quinone system was composed only of MK-6. Strain XC0022T possessed iso-C15 : 0, iso-C17 : 0 3-OH, C18 : 1ω9c and summed feature 3 (iso-C15 : 0 2-OH/C16 : 1ω7c) as the major fatty acids. The polar lipids profile consisted of one phosphatidylethanolamine, one unidentified glycolipid, four unidentified aminolipids and two unidentified lipids. The G+C content of the genomic DNA was 29.7 mol%. On the basis of phenotypic, phylogenetic and chemotaxonomic data, strain XC0022T (=KCTC 52364T=MCCC 1K02723T) represents a novel species of the genus Chryseobacterium, for which the name Chryseobacterium lineare sp. nov. is proposed.
authors.affiliation: College of Life Sciences, Zhejiang University, Hangzhou 310058, PR China.
authors.forename: Zhe
authors.initials: Z
authors.lastname: Zhao
authors.affiliation: College of Life Sciences, Zhejiang University, Hangzhou 310058, PR China.
authors.forename: Yin-Qi
authors.initials: YQ
authors.lastname: Tu
authors.affiliation: College of Life Sciences, Zhejiang University, Hangzhou 310058, PR China.
authors.forename: Xia
authors.initials: X
authors.lastname: Shen
authors.affiliation: College of Life Sciences, Zhejiang University, Hangzhou 310058, PR China.
authors.forename: Shuai-Bo
authors.initials: SB
authors.lastname: Han
authors.affiliation: Ocean College, Zhejiang University, Hangzhou 310058, PR China.
authors.forename: Chong-Ya
authors.initials: CY
authors.lastname: Zhang
authors.affiliation: College of Life Sciences, Zhejiang Sci-Tech University, Hangzhou 310018, PR China.
authors.forename: Cong
authors.initials: C
authors.lastname: Sun
authors.affiliation: College of Life Sciences, Zhejiang University, Hangzhou 310058, PR China.
authors.forename: Min
authors.initials: M
authors.lastname: Wu
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D002681:China; D045247:Chryseobacterium / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D006017:Glycolipids / Q000737:chemistry; D009693:Nucleic Acid Hybridization; D010714:Phosphatidylethanolamines / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D045483:Rivers / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D006017:Glycolipids; D010714:Phosphatidylethanolamines; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; C483858:phosphatidylethanolamine; C042455:menaquinone 6