title: Antiallergic effect of the atomized extract of rhizome ofCurcuma longa, flowers ofCordia luteaand leaves ofAnnona muricata.
abstract: BACKGROUND
  Allergies are a problem that greatly affects the population, and hence the use of antiallergic medications is fairly widespread. However, these drugs have many adverse effects. The use of medicinal plants could be an option, but they need to be evaluated.
  
  
  OBJECTIVE
  This study was designed to evaluate the antiallergic effect of the atomized extract of rhizome ofCurcuma longa, flowers ofCordia lutea, and leaves ofAnnona muricata.
  
  
  METHODS
  Twenty-four New Zealand white albino rabbits were randomized into 2 groups. Group A received the atomized extract diluted in physiological saline (APS) and group B received it diluted in Freund's adjuvant (FA). Then, the back of each rabbit was divided into 4 quadrants. The A-I quadrant received only physiological saline. The A-I quadrants of each rabbit conformed the PS group. The following 3 quadrants received the APS in 10 μg/mL, 100 μg/mL, and 1,000 μg/mL, respectively. The B-I quadrant received only FA. The B-I quadrants of each rabbit conformed the FA group. The following 3 quadrants received the AFA in 10 μg/mL, 100 μg/mL, and 1,000 μg/mL, respectively. The occurrence of erythema and edema was recorded according to the Draize scoring system and the primary irritation index. After 72 hours, biopsies were performed.
  
  
  RESULTS
  The AFA group presented significantly less erythema and edema compared to the FA group (P<0.05). The histopathologic evaluation at 72 hours showed normal characteristics in the APS group.
  
  
  CONCLUSIONS
  Considering the clinical and histopathological signs, we conclude that the administration of the atomized extract of rhizome ofC. longa, flowers ofC. lutea, and leaves ofA. muricatalacks antigenic effect but could have an antiallergenic effect in a model of dermal irritation in rabbits.
authors.affiliation: Pharmacology Laboratory, Institute of Clinical Research, National University of San Marcos, Lima, Peru; Institute of Clinical Research, National University of San Marcos, Lima, Peru; Institute of Pathology, Faculty of Medicine, National University of San Marcos, Lima, Peru.
authors.forename: Jorge
authors.initials: J
authors.lastname: Arroyo-Acevedo
authors.affiliation: Laboratory of Pharmacology and Physiology, Faculty of Dentistry, National University of San Marcos, Lima, Peru; Graduate Unit, Faculty of Pharmacy and Biochemistry, National University of San Marcos, Lima, Peru.
authors.forename: Cesar
authors.initials: C
authors.lastname: Franco-Quino
authors.affiliation: Laboratory of Pharmacology and Physiology, Faculty of Dentistry, National University of San Marcos, Lima, Peru; Graduate Unit, Faculty of Pharmacy and Biochemistry, National University of San Marcos, Lima, Peru.
authors.forename: Eliberto
authors.initials: E
authors.lastname: Ruiz-Ramirez
authors.affiliation: Pharmacology Laboratory, Institute of Clinical Research, National University of San Marcos, Lima, Peru; Association for the Development of Student Research in Health Sciences (ADIECS), Lima, Peru.
authors.forename: Roberto
authors.initials: R
authors.lastname: Chávez-Asmat
authors.affiliation: School of Human Medicine, Faculty of Medicine, National University of San Marcos, Lima, Peru; Sociedad Científica de San Fernando (SCSF), Lima, Peru.
authors.forename: Andrea
authors.initials: A
authors.lastname: Anampa-Guzmán
authors.affiliation: Institute of Pathology, Faculty of Medicine, National University of San Marcos, Lima, Peru.
authors.forename: Ernesto
authors.initials: E
authors.lastname: Raéz-González
authors.affiliation: Faculty of Medicine, National University San Luis Gonzaga of Ica, Ica, Peru.
authors.forename: José
authors.initials: J
authors.lastname: Cabanillas-Coral
keywords: edema; erythema; irritation; rabbit