title: Notch4 inhibition reduces migration and invasion and enhances sensitivity to docetaxel by inhibiting Akt/fascin in pancreatic cancer cells.
abstract: Overexpression of Notch4 is associated with a variety of tumor types. Only sparse information exists on Notch4 expression in pancreatic cancer (PC). The present study demonstrated that Notch4 expression was significantly upregulated in PC cell lines compared with a non-transformed pancreatic epithelial cell line, HPDE6c-7. To investigate the possible role of Notch4 in PC cells, an RNA interference approach was used to silence Notch4 expression. The results revealed that small interfering RNA (siRNA) targeting Notch4 significantly impeded the viability, migration and invasion abilities of PC cellsin vitro. Downregulation of Notch4 with siRNA sensitized cells to the action of docetaxel. Furthermore, Notch4 downregulation enhanced the inhibition of Akt activation and the fascin expression induced by docetaxel in PC cells. Together, these data provide insight into the function of Notch4 and suggest that Notch4 may represent a new potential target for gene therapy in PC.
authors.affiliation: Institute of Tumor, School of Medicine, Taizhou University, Taizhou, Zhejiang 318000, P.R. China; Department of Gastroenterology, Taizhou University Affiliated Taizhou Municipal Hospital, Taizhou, Zhejiang 318000, P.R. China.
authors.forename: Cui-Juan
authors.initials: CJ
authors.lastname: Qian
authors.affiliation: Institute of Tumor, School of Medicine, Taizhou University, Taizhou, Zhejiang 318000, P.R. China.
authors.forename: Yi-Yi
authors.initials: YY
authors.lastname: Chen
authors.affiliation: Department of Gastroenterology, Taizhou University Affiliated Taizhou Municipal Hospital, Taizhou, Zhejiang 318000, P.R. China.
authors.forename: Xin
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Institute of Tumor, School of Medicine, Taizhou University, Taizhou, Zhejiang 318000, P.R. China.
authors.forename: Fu-Qiang
authors.initials: FQ
authors.lastname: Liu
authors.affiliation: Institute of Tumor, School of Medicine, Taizhou University, Taizhou, Zhejiang 318000, P.R. China.
authors.forename: Ting-Ting
authors.initials: TT
authors.lastname: Yue
authors.affiliation: Department of Gastroenterology, Taizhou University Affiliated Taizhou Municipal Hospital, Taizhou, Zhejiang 318000, P.R. China.
authors.forename: Bei
authors.initials: B
authors.lastname: Ye
authors.affiliation: Institute of Tumor, School of Medicine, Taizhou University, Taizhou, Zhejiang 318000, P.R. China.
authors.forename: Jun
authors.initials: J
authors.lastname: Yao
keywords: Notch4; cell viability; metastasis; pancreatic cancer; siRNA