title: Comparison of a Resonant Mirror Biosensor (IAsys) and a Quartz Crystal Microbalance (QCM) for the Study on Interaction between Paeoniae Radix 801 and Endothelin-1.
abstract: A resonant mirror biosensor, IAsys, and a quartz crystal microbalance (QCM) are known independently as surface sensitive analytical devices capable of label-free and in situ bioassays. In this study, an IAsys and a QCM are employed for a new study on the action mechanism of Paeoniae Radix 801 (P. radix 801) by detecting the specific interaction between P. radix 801 and endothelin-1 (ET-1). In the experiments, ET-1 was immobilized on the surfaces of the IAsys cuvette and the QCM substrate by surface modification techniques, and then P. radix 801 solution was contacted to the cuvette and the substrate, separately. Then, the binding and interaction process between P. radix 801 and ET-1 was monitored by IAsys and QCM, respectively. The experimental results showed that P. radix 801 binds ET-1 specifically. The IAsys and QCM response curves to the ET-1 immobilization and P. radix 801 binding are similar in reaction process, but different in binding profiles, reflecting different resonation principles. Although both IAsys and QCM could detect the interaction of P. radix 801 and ET-1 with high reproducibility and reliability through optimization of the ET-1 coating, the reproducibility and reliability obtained by IAsys are better than those obtained by QCM, since the QCM frequency is more sensitive to temperature fluctuations, atmospheric changes and mechanical disturbances. However, IAsys and QCM are generally potent and reliable tools to study the interaction of P. radix 801 and ET-1, and can conclusively be applied to the action mechanism of P. radix 801.
authors.affiliation: College of Medicine and Life Sciences, University of Jinan, Jinan 250022, P.R. China. chm_huangjd@ujn.edu.cn.
authors.forename: Jiadong
authors.initials: J
authors.lastname: Huang
authors.affiliation: College of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, P.R. China. linqing19851215@126.com.
authors.forename: Qing
authors.initials: Q
authors.lastname: Lin
authors.affiliation: College of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, P.R. China. yujinghua@vip.sina.com.
authors.forename: Jinghua
authors.initials: J
authors.lastname: Yu
authors.affiliation: College of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, P.R. China. chm_gesg@ujn.edu.cn.
authors.forename: Shenguang
authors.initials: S
authors.lastname: Ge
authors.affiliation: Medical College of Nankai University, Tianjin 300071, P.R. China. stellarli@nankai.edu.cn.
authors.forename: Jing
authors.initials: J
authors.lastname: Li
authors.affiliation: College of Life Science, Nankai University, Tianjin 300071, P.R. China. flyfish113@163.com.
authors.forename: Min
authors.initials: M
authors.lastname: Yu
authors.affiliation: College of Life Science, Nankai University, Tianjin 300071, P.R. China. zhaozixia@hotmail.com.
authors.forename: Zixia
authors.initials: Z
authors.lastname: Zhao
authors.affiliation: College of Life Science, Nankai University, Tianjin 300071, P.R. China. wangxsh2001@163.com.
authors.forename: Xinsheng
authors.initials: X
authors.lastname: Wang
authors.affiliation: College of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, P.R. China. 19840321xm@163.com.
authors.forename: Xiuming
authors.initials: X
authors.lastname: Zhang
authors.affiliation: College of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, P.R. China. hxr666666@126.com.
authors.forename: Xiaorui
authors.initials: X
authors.lastname: He
authors.affiliation: College of Medicine and Life Sciences, University of Jinan, Jinan 250022, P.R. China. yl860131@126.com.
authors.forename: Liang
authors.initials: L
authors.lastname: Yuan
authors.affiliation: Xiyuan Hospital, China Academy of Traditional Chinese Medicine, Beijing 100091, P.R. China. huijunyin@yahoo.com.cn.
authors.forename: Huijun
authors.initials: H
authors.lastname: Yin
authors.affiliation: Emeritus professor of Graduate School of Pharmaceutical Sciences, Tohoku University, Aramaki, Aoba-ku, Sendai 980-8578, Japan. osa@mars.dti.ne.jp.
authors.forename: Tetsuo
authors.initials: T
authors.lastname: Osa
authors.affiliation: Xiyuan Hospital, China Academy of Traditional Chinese Medicine, Beijing 100091, P.R. China. kjchen@public.bta.net.cn.
authors.forename: Keji
authors.initials: K
authors.lastname: Chen
authors.affiliation: College of Life Science, Nankai University, Tianjin 300071, P.R. China. yichenhuang1127@hotmail.com.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Chen
keywords: Endothelin-1 (ET-1); IAsys; Interaction; Paeoniae Radix 801; Quartz crystal microbalance; Resonant mirror biosensor