title: Evaluation In Vitro of Immunoregulatory Cytokines Secretion by Dendritic Cells in Mountain Skiers.
abstract: In vitro production of immunoregulatory cytokines (IFN-α, IL-31, TNF-β, IL-17A, IL-7, IL-1RA, IL-1α, IL-10, IL-15, IL-21, IL-22, IL-23, IL-27, and IL-9) by dendritic cell cultures was compared in ski athletes and healthy donors. Effect of prolonged intense physical exercise on secretory activity of immune cells was investigated. In both groups, secretion of IL-1RA, IL-10, IL-1α by dendritic cells was revealed, but there were significant differences in IL-1RA, IL-1α content (p<0.05) with lower level in the group of athletes. Production of IL-17A and IL-7 by dendritic cells in the group of athletes was not detected. In athletes, several proinflammatory cytokines (IFN-α, IL-31, and TNF-β) were secreted by cells in high concentrations, in contrast to the control group. In both groups, dendritic cells did not secrete IL-15, IL-21, IL-22, IL-23, IL-27, and IL-9.
authors.affiliation: Research Institute of Nutrition, Moscow, Russia.
authors.forename: V S
authors.initials: VS
authors.lastname: Evstratova
authors.affiliation: Research Institute of Nutrition, Moscow, Russia.
authors.forename: D B
authors.initials: DB
authors.lastname: Nikityuk
authors.affiliation: Research Institute of Nutrition, Moscow, Russia.
authors.forename: N A
authors.initials: NA
authors.lastname: Riger
authors.affiliation: Research Institute of Nutrition, Moscow, Russia.
authors.forename: N V
authors.initials: NV
authors.lastname: Fedyanina
authors.affiliation: Research Institute of Nutrition, Moscow, Russia. khanferyan@ion.ru.
authors.forename: R A
authors.initials: RA
authors.lastname: Khanferyan
mesh_terms: D000531:Altitude; D056352:Athletes; D003713:Dendritic Cells / Q000166:cytology / Q000276:immunology*; D015444:Exercise; D005786:Gene Expression Regulation / Q000276:immunology*; D006801:Humans; D016898:Interferon-alpha / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D020381:Interleukin-17 / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D015851:Interleukin-7 / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D007378:Interleukins / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D008233:Lymphotoxin-alpha / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D010807:Physical Endurance / Q000276:immunology*; D061251:Primary Cell Culture; D012426:Russia; D012865:Skiing; D055815:Young Adult
chemical_list: C508717:IL17A protein, human; C502187:IL31 protein, human; C508605:IL7 protein, human; D016898:Interferon-alpha; D020381:Interleukin-17; D015851:Interleukin-7; D007378:Interleukins; D008233:Lymphotoxin-alpha
keywords: cytokines; dendritic cells; peripheral blood mononuclear cells; sports, immunity