title: Characterisation of ZBTB46 and DC-SCRIPT/ZNF366 in rainbow trout, transcription factors potentially involved in dendritic cell maturation and activation in fish.
abstract: ZBTB46 and DC-SCRIPT/ZNF366 are two zinc finger transcription factors that play important roles in regulating differentiation of dendritic cells in mammals. In this study, the ZBTB46 and DC-SCRIPT/ZNF366 homologues were identified in rainbow trout Oncorhynchus mykiss and their expression analysed in vivo and in vitro. As transcription factors, they are well conserved in sequence, genomic organisation and gene synteny. Their expression was differentially modulated by bacterial and viral PAMPs in the monocyte/macrophage-like cell line RTS-11, in primary head kidney (HK) macrophages, and in HK macrophages cultured with IL-4/13A. In the RTS-11 cells and primary HK macrophages, all the ZBTB46 and DC-SCRIPT/ZNF366 homologues were down-regulated by interferon gamma (type II IFN) but unaffected by IFN2 (type I IFN), administered as recombinant proteins to cell cultures. In fish gills, infection with amoebae (Paramoebae perurans) resulted in reduction of ZBTB46 and DC-SCRIPT/ZNF366 expression in Atlantic salmon Salmo salar, whilst infection with Yersinia ruckeri induced gene expression in rainbow trout.
authors.affiliation: Scottish Fish Immunology Research Centre, Institute of Biological and Environmental Sciences, University of Aberdeen, Aberdeen AB24 2TZ, UK; Department of Basic Veterinary, Sichuan Agricultural University, Huimin Road No. 211, Chengdu 611130, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Wang
authors.affiliation: Scottish Fish Immunology Research Centre, Institute of Biological and Environmental Sciences, University of Aberdeen, Aberdeen AB24 2TZ, UK.
authors.forename: Tiehui
authors.initials: T
authors.lastname: Wang
authors.affiliation: Scottish Fish Immunology Research Centre, Institute of Biological and Environmental Sciences, University of Aberdeen, Aberdeen AB24 2TZ, UK.
authors.forename: Ottavia
authors.initials: O
authors.lastname: Benedicenti
authors.affiliation: Marine Scotland Science Marine Laboratory, 375 Victoria Rd, Aberdeen AB11 9DB, UK.
authors.forename: Catherine
authors.initials: C
authors.lastname: Collins
authors.affiliation: Department of Basic Veterinary, Sichuan Agricultural University, Huimin Road No. 211, Chengdu 611130, China.
authors.forename: Kaiyu
authors.initials: K
authors.lastname: Wang
authors.affiliation: Scottish Fish Immunology Research Centre, Institute of Biological and Environmental Sciences, University of Aberdeen, Aberdeen AB24 2TZ, UK.
authors.forename: Christopher J
authors.initials: CJ
authors.lastname: Secombes
authors.affiliation: Scottish Fish Immunology Research Centre, Institute of Biological and Environmental Sciences, University of Aberdeen, Aberdeen AB24 2TZ, UK. Electronic address: j.j.zou@abdn.ac.uk.
authors.forename: Jun
authors.initials: J
authors.lastname: Zou
mesh_terms: D000562:Amebiasis / Q000276:immunology*; D000656:Amoeba / Q000276:immunology*; D000818:Animals; D002352:Carrier Proteins / Q000235:genetics* / Q000378:metabolism; D002454:Cell Differentiation; D002460:Cell Line; D003001:Cloning, Molecular; D003713:Dendritic Cells / Q000502:physiology*; D005393:Fish Diseases / Q000276:immunology*; D029941:Fish Proteins / Q000235:genetics* / Q000378:metabolism; D005786:Gene Expression Regulation / Q000276:immunology; D060226:Head Kidney / Q000473:pathology*; D007113:Immunity, Innate; D007370:Interferon Type I / Q000378:metabolism; D007371:Interferon-gamma / Q000378:metabolism; D008264:Macrophages / Q000276:immunology*; D017686:Oncorhynchus mykiss / Q000276:immunology*; D000069452:Pathogen-Associated Molecular Pattern Molecules / Q000276:immunology; D020317:Salmo salar / Q000276:immunology*; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism; D015009:Yersinia Infections / Q000276:immunology*; D044124:Yersinia ruckeri / Q000276:immunology*
chemical_list: D002352:Carrier Proteins; D029941:Fish Proteins; D007370:Interferon Type I; D000069452:Pathogen-Associated Molecular Pattern Molecules; D014157:Transcription Factors; C515832:ZNF366 protein, human; C574225:Zbtb46 protein, human; D007371:Interferon-gamma
keywords: DC-SCRIPT/ZNF366; Dendritic cell; Gene expression; Interferon; Transcription factor; ZBTB46