title: Daily ingestion of the probiotic Lactobacillus paracasei ST11 decreases Vaccinia virus dissemination and lethality in a mouse model.
abstract: Vaccinia virus (VACV) is an important pathogen. Although studies have shown relationships between probiotics and viruses, the effect of probiotics on VACV infection is unknown. Therefore, this work aims to investigate the probiotics effects on VACV infection. Mice were divided into four groups, two non-infected groups, one receiving the probiotic, the other one not receiving it, and two groups infected intranasally with VACV Western Reserve (VACV-WR) receiving or not receiving the probiotic. Viral titres in organs and cytokine production in the lungs were analysed. Lung samples were also subjected to histological analysis. The intake of probiotic results in reduction in viral spread with a significant decrease of VACV titer on lung, liver and brain of treated group. In addition,treatment with the probiotic results in attenuated mice lung inflammation showing fewer lesions on histological findings and decreased lethality in mice infected with VACV. The ingestion of Lactobacillus paracasei ST11 (LPST11) after VACV infection resulted in 2/9 animal lethality compared with 4/9 in the VACV group. This is the first study on probiotics and VACV interactions, providing not only information about this interaction, but also proposing a model for future studies involving probiotics and other poxvirus.
authors.affiliation: 1 Departamento de Microbiologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Av. Antônio Carlos 6627, 31270-901 Belo Horizonte, MG, Brazil.
authors.forename: A C
authors.initials: AC
authors.lastname: Dos Santos Pereira Andrade
authors.affiliation: 1 Departamento de Microbiologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Av. Antônio Carlos 6627, 31270-901 Belo Horizonte, MG, Brazil.
authors.forename: M Teixeira
authors.initials: MT
authors.lastname: Lima
authors.affiliation: 1 Departamento de Microbiologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Av. Antônio Carlos 6627, 31270-901 Belo Horizonte, MG, Brazil.
authors.forename: G Pereira
authors.initials: GP
authors.lastname: Oliveira
authors.affiliation: 1 Departamento de Microbiologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Av. Antônio Carlos 6627, 31270-901 Belo Horizonte, MG, Brazil.
authors.forename: R Silva
authors.initials: RS
authors.lastname: Calixto
authors.affiliation: 1 Departamento de Microbiologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Av. Antônio Carlos 6627, 31270-901 Belo Horizonte, MG, Brazil.
authors.forename: É Lorenna
authors.initials: ÉL
authors.lastname: de Sales E Souza
authors.affiliation: 1 Departamento de Microbiologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Av. Antônio Carlos 6627, 31270-901 Belo Horizonte, MG, Brazil.
authors.forename: D
authors.initials: D
authors.lastname: da Glória de Souza
authors.affiliation: 2 Departamento de Morfologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Av. Antônio Carlos 6627, 31270-901 Belo Horizonte, MG, Brazil.
authors.forename: C M
authors.initials: CM
authors.lastname: de Almeida Leite
authors.affiliation: 3 Laboratório de Microbiologia, Universidade Federal de São João del-Rei, Av. Sebastião Gonçalves Coelho 400, 35501-296 Divinópolis, MG, Brazil.
authors.forename: J M Siqueira
authors.initials: JM
authors.lastname: Ferreira
authors.affiliation: 1 Departamento de Microbiologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Av. Antônio Carlos 6627, 31270-901 Belo Horizonte, MG, Brazil.
authors.forename: E G
authors.initials: EG
authors.lastname: Kroon
authors.affiliation: 1 Departamento de Microbiologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Av. Antônio Carlos 6627, 31270-901 Belo Horizonte, MG, Brazil.
authors.forename: D Bretas
authors.initials: DB
authors.lastname: de Oliveira
authors.affiliation: 1 Departamento de Microbiologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Av. Antônio Carlos 6627, 31270-901 Belo Horizonte, MG, Brazil.
authors.forename: F
authors.initials: F
authors.lastname: Dos Santos Martins
authors.affiliation: 1 Departamento de Microbiologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Av. Antônio Carlos 6627, 31270-901 Belo Horizonte, MG, Brazil.
authors.forename: J S
authors.initials: JS
authors.lastname: Abrahão
mesh_terms: D000818:Animals; D016207:Cytokines / Q000032:analysis; D004195:Disease Models, Animal; D004435:Eating; D007249:Inflammation / Q000628:therapy; D000069980:Lacticaseibacillus paracasei / Q000502:physiology*; D008168:Lung / Q000473:pathology / Q000821:virology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D019936:Probiotics; D014615:Vaccinia / Q000628:therapy*; D014616:Vaccinia virus / Q000502:physiology*
chemical_list: D016207:Cytokines
keywords: Orthopoxvirus; inflammation; probiotics; virus