title: POWERDRESS interacts with HISTONE DEACETYLASE 9 to promote aging inArabidopsis.
abstract: Leaf senescence is an essential part of the plant lifecycle during which nutrients are re-allocated to other tissues. The regulation of leaf senescence is a complex process. However, the underlying mechanism is poorly understood. Here, we uncovered a novel and the pivotal role ofArabidopsisHDA9 (a RPD3-like histone deacetylase) in promoting the onset of leaf senescence. We found that HDA9 acts in complex with a SANT domain-containing protein POWERDRESS (PWR) and transcription factor WRKY53. Our genome-wide profiling of HDA9 occupancy reveals that HDA9 directly binds to the promoters of key negative regulators of senescence and this association requires PWR. Furthermore, we found that PWR is important for HDA9 nuclear accumulation. This study reveals an uncharacterized epigenetic complex involved in leaf senescence and provides mechanistic insights into how a histone deacetylase along with a chromatin-binding protein contribute to a robust regulatory network to modulate the onset of plant aging.
authors.affiliation: Laboratory of Genetics, University of Wisconsin-Madison, Madison, United States.|Wisconsin Institutes for Discovery, University of Wisconsin-Madison, Madison, United States.
authors.forename: Xiangsong
authors.initials: X
authors.lastname: Chen
authors.affiliation: Laboratory of Genetics, University of Wisconsin-Madison, Madison, United States.|Wisconsin Institutes for Discovery, University of Wisconsin-Madison, Madison, United States.
authors.forename: Li
authors.initials: L
authors.lastname: Lu
authors.affiliation: Laboratory of Genetics, University of Wisconsin-Madison, Madison, United States.|Wisconsin Institutes for Discovery, University of Wisconsin-Madison, Madison, United States.
authors.forename: Kevin S
authors.initials: KS
authors.lastname: Mayer
authors.affiliation: Department of Chemistry, University of Wisconsin-Madison, Madison, United States.
authors.forename: Mark
authors.initials: M
authors.lastname: Scalf
authors.affiliation: Laboratory of Genetics, University of Wisconsin-Madison, Madison, United States.|Wisconsin Institutes for Discovery, University of Wisconsin-Madison, Madison, United States.
authors.forename: Shuiming
authors.initials: S
authors.lastname: Qian
authors.affiliation: Laboratory of Genetics, University of Wisconsin-Madison, Madison, United States.
authors.forename: Aaron
authors.initials: A
authors.lastname: Lomax
authors.affiliation: Department of Chemistry, University of Wisconsin-Madison, Madison, United States.
authors.forename: Lloyd M
authors.initials: LM
authors.lastname: Smith
authors.affiliation: Laboratory of Genetics, University of Wisconsin-Madison, Madison, United States.|Wisconsin Institutes for Discovery, University of Wisconsin-Madison, Madison, United States.
authors.forename: Xuehua
authors.identifier: 0000-0002-2350-0046
authors.initials: X
authors.lastname: Zhong
mesh_terms: D017360:Arabidopsis / Q000502:physiology*; D029681:Arabidopsis Proteins / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D006655:Histone Deacetylases / Q000378:metabolism*; D055503:Protein Multimerization; D014157:Transcription Factors / Q000378:metabolism*
chemical_list: D029681:Arabidopsis Proteins; D004268:DNA-Binding Proteins; C581301:POWERDRESS protein, Arabidopsis; D014157:Transcription Factors; C496136:WRKY53 protein, Arabidopsis; C583167:HDA9 protein, Arabidopsis; D006655:Histone Deacetylases
keywords: A. thaliana; chromatin targeting; chromosomes; epigenetic regulation; genes; genome-wide profiling; histone deacetylase; plant aging; plant biology; transcription factors