title: Erythrocyte membrane based cationic polymer-mcDNA complexes as an efficient gene delivery system.
abstract: Gene therapy has great promise for the treatment of obtained and inherited serious diseases. However, the lack of safe and efficient gene delivery systems remains a barrier for their clinical application. Here, we reported a potential gene delivery vehicle composed of the erythrocyte membrane and cationic polymers, for example the XtremeGENE from Roche and the ε-caprolactone modified polyethylenimine. In addition to high efficiency, this system showed negligible cytotoxicity compared to the two cationic polymers alone in various cell lines, including human embryonic kidney cells (293T), human liver cancer cells (Huh7 and HepG2), murine dendritic cells (DC2.4) and human umbilical cord mesenchymal stem cells (Hu-MSCs). Moreover, the results of confocal laser scanning microscopy and flow cytometry suggested that the cell uptake of this gene vector was improved and might be introduced by the fusion interaction between the erythrocyte membrane and targeted cells.Thus, all the results revealed that the erythrocyte membrane based gene delivery system might be able to serve as an excellent gene delivery system.
authors.affiliation: Center for Gene and Cell Engineering, Institute of Biomedicine and Biotechnology, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, P. R. China. zy.chen1@siat.ac.cn.
authors.forename: Ping
authors.initials: P
authors.lastname: Huang
authors.affiliation: Center for Gene and Cell Engineering, Institute of Biomedicine and Biotechnology, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, P. R. China. zy.chen1@siat.ac.cn.
authors.forename: Jing
authors.initials: J
authors.lastname: Zhao
authors.affiliation: Multidisciplinary Research Center, Shantou 515063, P. R. China. chijuwei@stu.edu.cn.
authors.forename: Chiju
authors.initials: C
authors.lastname: Wei
authors.affiliation: Center for Gene and Cell Engineering, Institute of Biomedicine and Biotechnology, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, P. R. China. zy.chen1@siat.ac.cn.
authors.forename: Xiaohu
authors.initials: X
authors.lastname: Hou
authors.affiliation: Center for Gene and Cell Engineering, Institute of Biomedicine and Biotechnology, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, P. R. China. zy.chen1@siat.ac.cn.
authors.forename: Pingzhang
authors.initials: P
authors.lastname: Chen
authors.affiliation: Paul C. Lauterbur Research Center for Biomedical Imaging, Shenzhen Key Laboratory for MRI, Institute of Biomedical and Health Engineering, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, P. R. China. zy.wang@siat.ac.cn.
authors.forename: Yan
authors.initials: Y
authors.lastname: Tan
authors.affiliation: Center for Gene and Cell Engineering, Institute of Biomedicine and Biotechnology, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, P. R. China. zy.chen1@siat.ac.cn.
authors.forename: Cheng-Yi
authors.initials: CY
authors.lastname: He
authors.affiliation: Center for Gene and Cell Engineering, Institute of Biomedicine and Biotechnology, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, P. R. China. zy.chen1@siat.ac.cn and Paul C. Lauterbur Research Center for Biomedical Imaging, Shenzhen Key Laboratory for MRI, Institute of Biomedical and Health Engineering, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, P. R. China. zy.wang@siat.ac.cn.
authors.forename: Zhiyong
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Center for Gene and Cell Engineering, Institute of Biomedicine and Biotechnology, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, P. R. China. zy.chen1@siat.ac.cn.
authors.forename: Zhi-Ying
authors.initials: ZY
authors.lastname: Chen
mesh_terms: D000818:Animals; D002208:Caproates / Q000737:chemistry*; D002460:Cell Line; D004247:DNA / Q000737:chemistry; D004910:Erythrocyte Membrane / Q000737:chemistry*; D018014:Gene Transfer Techniques; D006801:Humans; D007783:Lactones / Q000737:chemistry*; D051379:Mice; D011094:Polyethyleneimine / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry*
chemical_list: D002208:Caproates; D007783:Lactones; D011108:Polymers; C121056:caprolactone; D011094:Polyethyleneimine; D004247:DNA