title: Goat umbilical cord cells are permissive to small ruminant lentivirus infection in vitro.
abstract: Small ruminant lentiviruses isolated from peripheral blood leukocytes and target organs can be propagated in vitro in fibroblasts derived from goat synovial membrane cells. These cells are obtained from tissues collected from embryos or fetuses and are necessary for the establishment of the fibroblast primary culture. A new alternative type of host cells, derived from goat umbilical cord, was isolated and characterized phenotypically with its main purpose being to obtain cell monolayers that could be used for the diagnosis and isolation of small ruminant lentiviruses in cell culture. To accomplish this goal, cells were isolated from umbilical cords; characterized phenotypically by flow cytometry analysis; differentiate into osteogenic, chondrogenic and adipogenic lineage; and submitted to viral challenge. The proliferation of goat umbilical cord cells was fast and cell monolayers formed after 15 days. These cells exhibited morphology, immunophenotype, growth characteristics, and lineage differentiation potential similar to mesenchymal stem cells of other origins. The goat umbilical cord derived cells stained positive for vimentin and CD90, but negative for cytokeratin, CD34 and CD105 markers. Syncytia and cell lysis were observed in cell monolayers infected by CAEV-Cork and MVV-K1514, showing that the cells are permissive to small ruminant lentivirus infection in vitro. These data demonstrate the proliferative competence of cells derived from goat umbilical cords and provide a sound basis for future research to standardize this cell lineage.
authors.affiliation: Universidade Estadual do Ceará, Programa de Pós-graduação em Ciências Veterinárias, Laboratório de Virologia, Fortaleza, CE, Brazil. Electronic address: rmgabrielle@hotmail.com.
authors.forename: Gabrielle R
authors.initials: GR
authors.lastname: Martins
authors.affiliation: Universidade Estadual do Ceará, Programa de Pós-graduação em Ciências Veterinárias, Laboratório de Virologia, Fortaleza, CE, Brazil.
authors.forename: Rebeca C
authors.initials: RC
authors.lastname: Marinho
authors.affiliation: Universidade Estadual do Ceará, Programa de Pós-graduação em Ciências Veterinárias, Laboratório de Virologia, Fortaleza, CE, Brazil.
authors.forename: Rosivaldo Q Bezerra
authors.initials: RQ
authors.lastname: Junior
authors.affiliation: Universidade Estadual do Ceará, Programa de Pós-graduação em Ciências Veterinárias, Laboratório de Virologia, Fortaleza, CE, Brazil.
authors.forename: Antoniel de O
authors.initials: AO
authors.lastname: Alves
authors.affiliation: Universidade Federal do Ceará, Laboratório de Imunologia, Programa de Pós-graduação em Microbiologia Médica, Fortaleza, CE, Brazil.
authors.forename: Lilia M C
authors.initials: LM
authors.lastname: Câmara
authors.affiliation: Universidade Federal do Ceará, Laboratório de Imunologia, Programa de Pós-graduação em Microbiologia Médica, Fortaleza, CE, Brazil.
authors.forename: Luiz C
authors.initials: LC
authors.lastname: Albuquerque-Pinto
authors.affiliation: Universidade Estadual do Ceará, Programa de Pós-graduação em Ciências Veterinárias, Laboratório de Virologia, Fortaleza, CE, Brazil.
authors.forename: Maria F da S
authors.initials: MF
authors.lastname: Teixeira
mesh_terms: D050156:Adipogenesis; D000818:Animals; D015415:Biomarkers; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D002478:Cells, Cultured; D020219:Chondrogenesis; D003588:Cytopathogenic Effect, Viral; D006041:Goats; D016130:Immunophenotyping; D066298:In Vitro Techniques; D016086:Lentivirus / Q000502:physiology*; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism / Q000473:pathology / Q000821:virology*; D010012:Osteogenesis; D014470:Umbilical Cord / Q000166:cytology*; D014779:Virus Replication
chemical_list: D015415:Biomarkers
keywords: CAEV; Cell culture; Flow cytometry; MSC; MVV