title: Effect of amino groups of mesoporous silica nanoparticles on CpG oligodexynucleotide delivery.
abstract: In this study, we proposed to modify mesoporous silica nanoparticles (MSNs) with 3-aminopropyltriethoxysilane (NH2-TES), aminoethylaminopropyltriethoxysilane (2NH2-TES) and 3-[2-(2-aminoethylamino)ethylamino] propyl-trimethoxysilane (3NH2-TES) for binding of cytosine-phosphate-guanosine oligodexynucleotides (CpG ODN), and investigated the effect of different amino groups of MSNs on the CpG ODN delivery. Serum stability,in vitrocytotoxicity, and cytokine interleukin-6 (IL-6) induction by MSN-NH2/CpG, MSN-2NH2/CpG and MSN-3NH2/CpG complexes were investigated in detail. The results showed that three kinds of aminated-MSN-based CpG ODN delivery systems had no cytotoxicity to RAW264.7 cells, and binding of CpG ODN to MSN-NH2, MSN-2NH2and MSN-3NH2nanoparticles enhanced the serum stability of CpG ODN due to protection by the nanoparticles. However, three aminated MSN-based CpG ODN delivery systems exhibited different CpG ODN delivery efficiency, and MSN-NH2/CpG complexes had the highest ability to induce IL-6 secretion.
authors.affiliation: School of Medical Instrument and Food Engineering, University of Shanghai for Science and Technology, 516 Jungong Road, Shanghai 200093, People's Republic of China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Xu
authors.affiliation: School of Engineering, Sino-British College (USST), 1195 Fuxing Zhong Road, Shanghai 200031, People's Republic of China.
authors.forename: Peter
authors.initials: P
authors.lastname: Claiden
authors.affiliation: School of Materials Science and Engineering, University of Shanghai for Science and Technology, 516 Jungong Road, Shanghai 200093, People's Republic of China.
authors.forename: Yufang
authors.initials: Y
authors.lastname: Zhu
authors.affiliation: Nanotechnology Innovation Station, National Institute for Materials Science, 1-2-1 Sengen, Tsukuba, Ibaraki 305-0047, Japan.
authors.forename: Hiromi
authors.initials: H
authors.lastname: Morita
authors.affiliation: Nanotechnology Innovation Station, National Institute for Materials Science, 1-2-1 Sengen, Tsukuba, Ibaraki 305-0047, Japan.
authors.forename: Nobutaka
authors.initials: N
authors.lastname: Hanagata
keywords: CpG delivery; amination; cytokine induction; mesoporous silica