title: A Tightly Regulated Genetic Selection System with Signaling-Active Alleles of Phytochrome B.
abstract: Selectable markers derived from plant genes circumvent the potential risk of antibiotic/herbicide-resistance gene transfer into neighboring plant species, endophytic bacteria, and mycorrhizal fungi. Toward this goal, we have engineered and validated signaling-active alleles of phytochrome B (eYHB) as plant-derived selection marker genes in the model plant Arabidopsis (Arabidopsis thaliana). By probing the relationship of construct size and induction conditions to optimal phenotypic selection, we show that eYHB-based alleles are robust substitutes for antibiotic/herbicide-dependent marker genes as well as surprisingly sensitive reporters of off-target transgene expression.
authors.affiliation: Department of Molecular and Cellular Biology, University of California, Davis, California 95776.
authors.forename: Wei
authors.identifier: 0000-0001-9602-1428
authors.initials: W
authors.lastname: Hu
authors.affiliation: Department of Molecular and Cellular Biology, University of California, Davis, California 95776 jclagarias@ucdavis.edu.
authors.forename: J Clark
authors.identifier: 0000-0002-2093-0403
authors.initials: JC
authors.lastname: Lagarias
mesh_terms: D000483:Alleles; D017360:Arabidopsis / Q000235:genetics*; D005818:Genetic Engineering / Q000379:methods*; D018869:Heat-Shock Response / Q000235:genetics; D010641:Phenotype; D051939:Phytochrome B / Q000235:genetics*; D030821:Plants, Genetically Modified; D012641:Selection, Genetic; D015398:Signal Transduction / Q000235:genetics*; D014170:Transformation, Genetic
chemical_list: D051939:Phytochrome B