title: Influence of Mesenchymal Stem Cells Conditioned Media on Proliferation of Urinary Tract Cancer Cell Lines and Their Sensitivity to Ciprofloxacin.
abstract: Mesenchymal stem cells (MSCs) are known to interact with cancer cells through direct cell-to-cell contact and secretion of paracrine factors, although their exact influence on tumor progression in vivo remains unclear. To better understand how fetal and adult stem cells affect tumors, we analyzed viability of human renal (786-0) and bladder (T24) carcinoma cell lines cultured in conditioned media harvested from amniotic fluid-derived stem cells (AFSCs) and adipose-derived stem cells (ASCs). Both media reduced metabolic activity of 786-0 cells, however, decreased viability of T24 cells was noted only after incubation with conditioned medium from ASCs. To test the hypothesis that MSCs-secreted factors might be involved in chemoresistance acquisition, we further analyzed influence of mesenchymal stem cell conditioned media (MSC-CM) on cancer cells sensitivity to ciprofloxacin, that is considered as potential candidate agent for urinary tract cancers treatment. Significantly increased resistance to tested drug indicates that MSCs may protect cancer cells from chemotherapy. J. Cell. Biochem. 118: 1361-1368, 2017. © 2016 Wiley Periodicals, Inc.
authors.affiliation: Chair of Urology, Department of Tissue Engineering, Collegium Medicum, Nicolaus Copernicus University, Karlowicza 24, 85-092, Bydgoszcz, Poland.
authors.forename: Malgorzata
authors.initials: M
authors.lastname: Maj
authors.affiliation: Chair of Urology, Department of Tissue Engineering, Collegium Medicum, Nicolaus Copernicus University, Karlowicza 24, 85-092, Bydgoszcz, Poland.
authors.forename: Anna
authors.initials: A
authors.lastname: Bajek
authors.affiliation: Chair of Urology, Department of Tissue Engineering, Collegium Medicum, Nicolaus Copernicus University, Karlowicza 24, 85-092, Bydgoszcz, Poland.
authors.forename: Ewelina
authors.initials: E
authors.lastname: Nalejska
authors.affiliation: Department of Biochemistry, Nicolaus Copernicus University, Gagarina 7, 87-100, Torun, Poland.
authors.forename: Dorota
authors.initials: D
authors.lastname: Porowinska
authors.affiliation: Chair of Urology, Department of Regenerative Medicine, Collegium Medicum, Nicolaus Copernicus University, Sklodowskiej-Curie 9, 85-094, Bydgoszcz, Poland.
authors.forename: Tomasz
authors.initials: T
authors.lastname: Kloskowski
authors.affiliation: Department of Immunology, Collegium Medicum, Nicolaus Copernicus University, Sklodowskiej-Curie 9, 85-094, Bydgoszcz, Poland.
authors.forename: Lidia
authors.initials: L
authors.lastname: Gackowska
authors.affiliation: Chair of Urology, Clinic of General and Oncological Urology, Collegium Medicum, Nicolaus Copernicus University, Sklodowskiej-Curie 9, 85-094, Bydgoszcz, Poland.|Department of Urology, Nicolaus Copernicus Hospital, Batorego 17/19, 87-100, Torun, Poland.
authors.forename: Tomasz
authors.initials: T
authors.lastname: Drewa
mesh_terms: D000273:Adipose Tissue / Q000166:cytology / Q000378:metabolism; D000653:Amniotic Fluid / Q000166:cytology / Q000378:metabolism; D002453:Cell Cycle / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002939:Ciprofloxacin / Q000494:pharmacology*; D018920:Coculture Techniques; D017077:Culture Media, Conditioned / Q000494:pharmacology*; D019008:Drug Resistance, Neoplasm; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism*; D014571:Urologic Neoplasms / Q000378:metabolism*
chemical_list: D017077:Culture Media, Conditioned; D002939:Ciprofloxacin
keywords: ADIPOSE-DERIVED STEM CELLS; AMNIOTIC FLUID-DERIVED STEM CELLS; CHEMORESISTANCE; CIPROFLOXACIN; CONDITIONED MEDIUM; URINARY CANCER CELL LINES