title: MicroRNA-145 influences the balance of Th1/Th2 via regulating RUNX3 in asthma patients.
abstract: To delineate the underlying mechanism of microRNA-145 modulate the balance of Th1/Th2 via targeting RUNX3 in asthma patients.
  
  
  
  Peripheral blood samples were collected from asthma patients and healthy controls. CD4+T cells were isolated and cultured. Using quantitative PCR detect, the level of microRNA-145 and RUNX3 mRNA level in the CD4+T cells from asthma patients and healthy controls, meanwhile, western blot was used to detect the RUNX3 protein level. Th1 or Th2 related cytokines were measured by enzyme-linked immunosorbent assay. Dual-Luciferase Reporter Assay was performed to confirm the correlation between microRNA-145 and RUNX3. MicroRNA-145 mimic or inhibitor was transfected in the CD4+T cells and the changes of RUNX3 level, Th1 or Th2 related cytokines and the percentage of Th1 and Th2 were observed after transfection.
  
  
  
  MicroRNA-145 level of CD4+T cells was higher with a lower RUNX3 expression in asthma patients. There is negative correlation between microRNA-145 and RUNX3. Th2 hyperactivity and Th1 deficiency was detected in the CD4+T cells of asthma patients. Dual-Luciferase Reporter Assay has shown that RUNX3 is a target of microRNA. Up-regulation or down-regulation of miR-145 level caused RUNX3 expression changes in CD4+T cells and influence the related cytokines. Inhibition of microRNA-145 may reverse the imbalance of Th1/Th2 in asthma patients.
  
  
  
  MicroRNA-145 could regulate the balance of Th1/Th2 through targeting the RUNX3 in asthma patients. MicroRNA-145 and RUNX3 may be used as biomarkers or targets in the diagnosis or therapy of asthma.
authors.affiliation: a Respiratory Department in the Cadre Ward , Gansu Provincial Hospital , Lanzhou , China.
authors.forename: Linxia
authors.initials: L
authors.lastname: Fan
authors.affiliation: b Respiratory Department , Gansu Provincial Hospital , Lanzhou , China.
authors.forename: Xiaojun
authors.initials: X
authors.lastname: Wang
authors.affiliation: c Medical Experimental Center of Lanzhou University , Lanzhou , China.
authors.forename: Linlan
authors.initials: L
authors.lastname: Fan
authors.affiliation: a Respiratory Department in the Cadre Ward , Gansu Provincial Hospital , Lanzhou , China.
authors.forename: Qizhang
authors.initials: Q
authors.lastname: Chen
authors.affiliation: d Asthma Center of the Gansu Provincial Hospital , Lanzhou , China.
authors.forename: Hong
authors.initials: H
authors.lastname: Zhang
authors.affiliation: e Department of Internet Information , Gansu Provincial Hospital , Lanzhou , China.
authors.forename: Hui
authors.initials: H
authors.lastname: Pan
authors.affiliation: f Department of Pharmacy , Gansu Provincial Hospital , Lanzhou , China.
authors.forename: Aixia
authors.initials: A
authors.lastname: Xu
authors.affiliation: a Respiratory Department in the Cadre Ward , Gansu Provincial Hospital , Lanzhou , China.
authors.forename: Hongjuan
authors.initials: H
authors.lastname: Wang
authors.affiliation: g Department of Internal Medicine , Traditional Chinese Medicine Hospital of Lintao County , Lintao , China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Yu
mesh_terms: D001249:Asthma / Q000097:blood / Q000378:metabolism*; D018791:CD4 Lymphocyte Count; D015496:CD4-Positive T-Lymphocytes / Q000166:cytology; D016022:Case-Control Studies; D002478:Cells, Cultured; D050677:Core Binding Factor Alpha 3 Subunit / Q000378:metabolism*; D006801:Humans; D035683:MicroRNAs / Q000502:physiology*; D058408:Th1-Th2 Balance; D014162:Transfection
chemical_list: D050677:Core Binding Factor Alpha 3 Subunit; C541010:MIRN145 microRNA, human; D035683:MicroRNAs; C493729:Runx3 protein, human
keywords: RUNX3; T cells; asthma; microRNA