title: Low prevalence of Aichi virus in molluscan shellfish samples from Galicia (NW Spain).
abstract: OBJECTIVE
  The aim of this study was to detect and quantify Aichi virus (AiV) in shellfish from three estuaries in Galicia, the main producer of molluscs in Europe.
  
  
  RESULTS
  A total of 249 shellfish samples were analysed using a reverse transcription-quantitative PCR procedure. AiV was detected in 15 of 249 (6·02%) samples. Ría de Ares-Betanzos showed the highest prevalence (11·1%), followed by Ría do Burgo (3·7%) and Ría de Vigo, (2·56%). AiV quantifications ranged from nonquantifiable (under the limit of quantification of the method) to 6·9 × 103RNAc per g DT, with a mean value of 1·9 × 102RNAc per g DT.
  
  
  CONCLUSIONS
  Results obtained indicated that the prevalence of this enteric virus in the studied area is considerably lower than those of other enteric viruses, such as Norovirus, Sapovirus, HAV or HEV.
  
  
  CONCLUSIONS
  This is the first study that detects the presence of AiV in shellfish from authorized harvesting areas in Spain. Further studies with clinical samples are needed to determine the potential risk of AiV for human health in Galicia.
authors.affiliation: Departamento de Microbiología y Parasitología, CIBUS-Facultad de Biología, Universidade de Santiago de Compostela, Santiago de Compostela, Spain.
authors.forename: E
authors.initials: E
authors.lastname: Rivadulla
authors.affiliation: Departamento de Microbiología y Parasitología, CIBUS-Facultad de Biología, Universidade de Santiago de Compostela, Santiago de Compostela, Spain.
authors.forename: M F
authors.initials: MF
authors.lastname: Varela
authors.affiliation: Departamento de Microbiología y Parasitología, CIBUS-Facultad de Biología, Universidade de Santiago de Compostela, Santiago de Compostela, Spain.
authors.forename: J L
authors.initials: JL
authors.lastname: Romalde
mesh_terms: D000818:Animals; D005759:Gastroenteritis / Q000453:epidemiology / Q000821:virology*; D006801:Humans; D052699:Kobuvirus / Q000502:physiology*; D008974:Mollusca / Q000821:virology*; D015995:Prevalence; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012758:Shellfish / Q000821:virology*; D013030:Spain / Q000453:epidemiology
keywords: Aichi virus; RT-qPCR; bivalve molluscs; detection; prevalence