title: Dysregulation of hepatic microRNA expression profiles with Clonorchis sinensis infection.
abstract: BACKGROUND
  Clonorchiasis remains an important zoonotic parasitic disease worldwide. The molecular mechanisms of host-parasite interaction are not fully understood. Non-coding microRNAs (miRNAs) are considered to be key regulators in parasitic diseases. The regulation of miRNAs and host micro-environment may be involved in clonorchiasis, and require further investigation.
  
  
  METHODS
  MiRNA microarray technology and bioinformatic analysis were used to investigate the regulatory mechanisms of host miRNA and to compare miRNA expression profiles in the liver tissues of control and Clonorchis sinensis (C. sinensis)-infected rats.
  
  
  RESULTS
  A total of eight miRNAs were downregulated and two were upregulated, which showed differentially altered expression profiles in the liver tissue of C. sinensis-infected rats. Further analysis of the differentially expressed miRNAs revealed that many important signal pathways were triggered after infection with C. sinensis, which were related to clonorchiasis pathogenesis, such as cell apoptosis and inflammation, as well as genes involved in signal transduction mechanisms, such as pathways in cancer and the Wnt and Mitogen-activated protein kinases (MAPK) signaling pathways.
  
  
  CONCLUSIONS
  The present study revealed that the miRNA expression profiles of the host were changed by C. sinensis infection. This dysregulation in miRNA expression may contribute to the etiology and pathophysiology of clonorchiasis. These results also provide new insights into the regulatory mechanisms of miRNAs in clonorchiasis, which may present potential targets for future C. sinensis control strategies.
authors.affiliation: Department of Parasitology, Harbin Medical University, Harbin, 150081, China.|National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention; Key Laboratory of Parasite and Vector Biology, Ministry of Health, MOH; National Center for International Research on Tropical Diseases; WHO Collaborating Center for Tropical Diseases, Shanghai, People's Republic of China.
authors.forename: Su
authors.initials: S
authors.lastname: Han
authors.affiliation: Department of Parasitology, Harbin Medical University, Harbin, 150081, China.
authors.forename: Qiaoran
authors.initials: Q
authors.lastname: Tang
authors.affiliation: Department of Gastroenterology, The First Affiliated Hospital of Harbin Medical University, Harbin, China.
authors.forename: Xi
authors.initials: X
authors.lastname: Lu
authors.affiliation: Department of Orthopedic Surgery, The fourth Affiliated Hospital of Harbin Medical University, Harbin, China.
authors.forename: Rui
authors.initials: R
authors.lastname: Chen
authors.affiliation: Department of Parasitology, Harbin Medical University, Harbin, 150081, China.
authors.forename: Yihong
authors.initials: Y
authors.lastname: Li
authors.affiliation: Department of Parasitology, Harbin Medical University, Harbin, 150081, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Shu
authors.affiliation: Department of Parasitology, Harbin Medical University, Harbin, 150081, China. zhangxiaoli1119@163.com.
authors.forename: Xiaoli
authors.initials: X
authors.lastname: Zhang
authors.affiliation: National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention; Key Laboratory of Parasite and Vector Biology, Ministry of Health, MOH; National Center for International Research on Tropical Diseases; WHO Collaborating Center for Tropical Diseases, Shanghai, People's Republic of China. caojp@yahoo.com.
authors.forename: Jianping
authors.initials: J
authors.lastname: Cao
mesh_terms: D000818:Animals; D003003:Clonorchiasis / Q000235:genetics*; D003004:Clonorchis sinensis / Q000472:pathogenicity; D015536:Down-Regulation; D005260:Female; D005786:Gene Expression Regulation; D006790:Host-Parasite Interactions / Q000235:genetics; D008099:Liver / Q000473:pathology / Q000502:physiology; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D020411:Oligonucleotide Array Sequence Analysis; D051381:Rats; D017208:Rats, Wistar; D015398:Signal Transduction / Q000235:genetics; D015854:Up-Regulation; D015047:Zoonoses
chemical_list: D035683:MicroRNAs
keywords: Clonorchis sinensis; Hepatic; Infection; MicroRNA