title: Motor coordination and balance measurements reveal differential pathogenicity of currently spreading enterovirus 71 strains in human SCARB2 transgenic mice.
abstract: Enterovirus 71 (EV71) has caused large-scale epidemics with neurological complications in the Asia-Pacific region. The C4a and B5 strains are the two major genotypes circulating in many countries recently. This study used a new protocol, a motor coordination task, to assess the differential pathogenicity of C4a and B5 strains in human SCARB2 transgenic mice. We found that the pathogenicity of C4a viruses was more severe than that of B5 viruses. Moreover, we discovered that an increased level of monocyte chemoattractant protein-1 was positively correlated with severely deficient motor function. This study provides a new method for evaluating EV71 infection in mice and distinguishing the severity of the symptoms caused by different clinical strains, which would contribute to studies of pathogenesis and development of vaccines and antivirals in EV71 infections.
authors.affiliation: Research Center for Emerging Viral Infections, Chang Gung University, Tao-Yuan, Taiwan, ROC.
authors.forename: Mei-Feng
authors.initials: MF
authors.lastname: Chen
authors.affiliation: Clinical Virology Laboratory, Department of Laboratory Medicine, Chang Gung Memorial Hospital, Tao-Yuan, Taiwan, ROC.|Graduate Institute of Biomedical Sciences, Chang Gung University, Tao-Yuan, Taiwan, ROC.|Department of Medical Biotechnology and Laboratory Science, College of Medicine, Chang Gung University, Tao-Yuan, Taiwan, ROC.|Research Center for Emerging Viral Infections, Chang Gung University, Tao-Yuan, Taiwan, ROC.
authors.forename: Shin-Ru
authors.initials: SR
authors.lastname: Shih
mesh_terms: D000818:Animals; D018932:Chemokine CCL2 / Q000378:metabolism; D029821:Enterovirus A, Human / Q000472:pathogenicity* / Q000502:physiology; D004769:Enterovirus Infections / Q000235:genetics / Q000378:metabolism / Q000503:physiopathology* / Q000821:virology*; D005838:Genotype; D006801:Humans; D051907:Lysosomal Membrane Proteins / Q000235:genetics* / Q000378:metabolism; D051379:Mice; D008813:Mice, Inbred ICR; D008822:Mice, Transgenic; D009043:Motor Activity; D051116:Receptors, Scavenger / Q000235:genetics* / Q000378:metabolism; D014774:Virulence
chemical_list: D018932:Chemokine CCL2; D051907:Lysosomal Membrane Proteins; D051116:Receptors, Scavenger; C074299:SCARB2 protein, human