title: [Nonculture techniques of microorganisms determination in amniotic fluid in patients with preterm prelabor rupture of membranes].
abstract: OBJECTIVE
  The preterm prelabor rupture of membranes is a serious obstetric complication that is frequently complicated by the presence of microorganisms in amniotic fluid. The aim of our work is to characterize current status of nonculture detection of microbial invasion into the amniotic cavity and the experience with the technique performed in University Hospital in Hradec Kralove.
  
  
  METHODS
  Original survey article.
  
  
  METHODS
  Institute of Clinical Biochemistry and Diagnostics - molecular biology department, University Hospital Hradec Kralove.
  
  
  CONCLUSIONS
  Application of nonculture techniques of microorganisms determination in amniotic fluid in patients with preterm prelabor rupture of membranes is currently available. According to the detection of genital mycoplasmas as the dominant pathogens in the amniotic fluid this technique should be regarded as the standard examination method in these patients.
authors.forename: R
authors.initials: R
authors.lastname: Kutová
authors.forename: L
authors.initials: L
authors.lastname: Plíšková
authors.forename: M
authors.initials: M
authors.lastname: Kacerovský
mesh_terms: D000653:Amniotic Fluid / Q000382:microbiology*; D005260:Female; D005322:Premature Rupture of Fetal Membranes / Q000382:microbiology; D006801:Humans; D011247:Pregnancy; D011251:Pregnancy Complications, Infectious / Q000382:microbiology
keywords: genital mycoplasmas; polymerase chain reaction bacterial DNA.; preterm labor