title: From PPROM to caul: The evolution of membrane rupture in mammals.
abstract: Rupture of the extraembryonic membranes that form the gestational sac in humans is a typical feature of human parturition. However, preterm premature rupture of membranes (PPROM) occurs in approximately 1% of pregnancies, and is a leading cause of preterm birth. Conversely, retention of an intact gestational sac during parturition in the form of a caul is a rare occurrence. Understanding the molecular and evolutionary underpinnings of these disparate phenotypes can provide insight into both normal pregnancy and PPROM. Using phylogenetic techniques we reconstructed the evolution of the gestational sac phenotype at parturition in 55 mammal species representing all major viviparous mammal groups. We infer the ancestral state in therians, eutherians, and primates, as in humans, is a ruptured gestational sac at parturition. We present evidence that intact membranes at parturition have evolved convergently in diverse mammals including horses, elephants, and bats. In order to gain insight into the molecular underpinnings of the evolution of enhanced membrane integrity we also used comparative genomics techniques to reconstruct the evolution of a subset of genes implicated in PPROM, and find that four genes (ADAMTS2,COL1A1,COL5A1,LEPRE1) show significant evidence of increased nonsynonymous rates of substitution on lineages with intact membranes as compared to those with ruptured membranes. Among these genes, we also discovered that 17 human SNPs are associated with or near amino acid replacement sites in those mammals with intact membranes. These SNPs are candidate functional variants within humans, which may play roles in both PPROM and/or the retention of the gestational sac at birth.
authors.affiliation: Center for Molecular Medicine and Genetics, Wayne State University School of Medicine, Detroit MI 48201, United States.
authors.forename: Gregory
authors.initials: G
authors.lastname: Stempfle
authors.affiliation: Center for Molecular Medicine and Genetics, Wayne State University School of Medicine, Detroit MI 48201, United States.
authors.forename: Michael R
authors.initials: MR
authors.lastname: McGowen
authors.affiliation: Center for Molecular Medicine and Genetics, Wayne State University School of Medicine, Detroit MI 48201, United States.
authors.forename: Jason A
authors.initials: JA
authors.lastname: Caravas
authors.affiliation: Center for Molecular Medicine and Genetics, Wayne State University School of Medicine, Detroit MI 48201, United States; Department of Obstetrics and Gynecology, Wayne State University School of Medicine, Detroit MI 48201, United States.
authors.forename: Derek E
authors.initials: DE
authors.lastname: Wildman
keywords: Evolution; Fetal membranes; Gestational sac; Mammals; PPROM; Positive selection