title: Definition and identification of small RNA sponges: Focus on miRNA sequestration.
abstract: Targeting RNAs appears as an important opportunity to modulate biological processes. Here, we overviewed critical parameters implied in RNAs competition to bind small RNAs. These competitions influence small RNA availability and thereby gene expression and cell fate. We focused on the ability of RNAs to sequester small RNA, mainly the microRNAs (miRNAs) and proposed experimental workflows to demonstrate the existence and activity of RNA-sponge. From this basic science, we detailed tailored oligonucleotides, developed to challenge the binding of small RNA. In vitro and in vivo, these tailored oligonucleotides efficiently restore small RNA activity by preventing their sequestration on RNA-sponges.
authors.affiliation: Centre National de la Recherche Scientifique (CNRS) Unité Mixte de Recherche (UMR) 6290, Institut de Génétique et Développement de Rennes, France; Université de Rennes 1, Rennes, France; Cancer Research Association (ARC) Labelled Team, France.
authors.forename: Mélodie
authors.initials: M
authors.lastname: Migault
authors.affiliation: Centre National de la Recherche Scientifique (CNRS) Unité Mixte de Recherche (UMR) 6290, Institut de Génétique et Développement de Rennes, France; Université de Rennes 1, Rennes, France; Cancer Research Association (ARC) Labelled Team, France.
authors.forename: Emmanuelle
authors.initials: E
authors.lastname: Donnou-Fournet
authors.affiliation: Centre National de la Recherche Scientifique (CNRS) Unité Mixte de Recherche (UMR) 6290, Institut de Génétique et Développement de Rennes, France; Université de Rennes 1, Rennes, France; Cancer Research Association (ARC) Labelled Team, France; Department of Medical Genomic, Rennes University Hospital, Rennes, France. Electronic address: mgaliber@univ-rennes1.fr.
authors.forename: Marie-Dominique
authors.initials: MD
authors.lastname: Galibert
authors.affiliation: Centre National de la Recherche Scientifique (CNRS) Unité Mixte de Recherche (UMR) 6290, Institut de Génétique et Développement de Rennes, France; Université de Rennes 1, Rennes, France; Cancer Research Association (ARC) Labelled Team, France. Electronic address: david.gilot@univ-rennes1.fr.
authors.forename: David
authors.initials: D
authors.lastname: Gilot
mesh_terms: D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism; D001667:Binding, Competitive; D045744:Cell Line, Tumor; D017930:Genes, Reporter; D022781:Hepatocytes / Q000166:cytology / Q000378:metabolism; D006801:Humans; D017909:Levivirus / Q000737:chemistry; D035683:MicroRNAs / Q000235:genetics* / Q000302:isolation & purification* / Q000378:metabolism; D020320:Oligoribonucleotides, Antisense / Q000138:chemical synthesis / Q000378:metabolism*; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D035683:MicroRNAs; D020320:Oligoribonucleotides, Antisense; D014764:Viral Proteins