title: A GC-MS method for the detection and quantitation of ten major drugs of abuse in human hair samples.
abstract: A sensitive analytical method has been developed in order to identify and quantify major drugs of abuse (DOA), namely morphine, codeine, 6-monoacetylmorphine, cocaine, ecgonine methyl ester, benzoylecgonine, amphetamine, methamphetamine, methylenedioxymethamphetamine and methylenedioxyamphetamine in human hair. Samples of hair were extracted with methanol under ultrasonication at 50°C after a three step rinsing process to remove external contamination and dirt hair. Derivatization with BSTFA was selected in order to increase detection sensitivity of GC/MS analysis. Optimization of derivatization parameters was based on experiments for the selection of derivatization time, temperature and volume of derivatising agent. Validation of the method included evaluation of linearity which ranged from 2 to 350ng/mg of hair mean concentration for all DOA, evaluation of sensitivity, accuracy, precision and repeatability. Limits of detection ranged from 0.05 to 0.46ng/mg of hair. The developed method was applied for the analysis of hair samples obtained from three human subjects and were found positive in cocaine, and opiates.
authors.affiliation: Laboratory of Forensic Medicine & Toxicology, Medical School, Aristotle University Thessaloniki, 54124, Greece.
authors.forename: A
authors.initials: A
authors.lastname: Orfanidis
authors.affiliation: Laboratory of Forensic Service of Ministry of Justice of Thessaloniki, Greece.
authors.forename: O
authors.initials: O
authors.lastname: Mastrogianni
authors.affiliation: School of Chemistry, Laboratory of Analytical Chemistry, Aristotle University Thessaloniki, 54124, Greece.
authors.forename: A
authors.initials: A
authors.lastname: Koukou
authors.affiliation: School of Chemistry, Laboratory of Analytical Chemistry, Aristotle University Thessaloniki, 54124, Greece.
authors.forename: G
authors.initials: G
authors.lastname: Psarros
authors.affiliation: Laboratory of Forensic Medicine & Toxicology, Medical School, Aristotle University Thessaloniki, 54124, Greece.
authors.forename: H
authors.initials: H
authors.lastname: Gika
authors.affiliation: School of Chemistry, Laboratory of Analytical Chemistry, Aristotle University Thessaloniki, 54124, Greece.
authors.forename: G
authors.initials: G
authors.lastname: Theodoridis
authors.affiliation: Laboratory of Forensic Medicine & Toxicology, Medical School, Aristotle University Thessaloniki, 54124, Greece. Electronic address: raikos@med.auth.gr.
authors.forename: N
authors.initials: N
authors.lastname: Raikos
mesh_terms: D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006197:Hair / Q000737:chemistry*; D006801:Humans; D013287:Illicit Drugs / Q000032:analysis*; D057230:Limit of Detection; D015813:Substance Abuse Detection / Q000379:methods*
chemical_list: D013287:Illicit Drugs
keywords: Derivatization; Drugs of abuse; GC–MS; Hair analysis