title: Vesicles for Signal Amplification in a Biosensor for the Detection of Low Antigen Concentrations.
abstract: The sensitivity of biosensors is often not sufficient to detect diagnostically relevant biomarker concentrations. In this paper we have utilized a Quartz Crystal Microbalance with Dissipation monitoring (QCM-D) to detect dissipative losses induced by the attachment of intact vesicles. We modified a sandwich assay by coupling the secondary antibodies to vesicles. This resulted in an increase of detection sensitivity, achieving a diagnostically relevant detection limit of 5 ng/ml or 30 pM antigens. In addition, we could combine the individual assay steps to decrease the total time to result in about 30 minutes.
authors.affiliation: ETH Zurich, Laboratory of Biosensors and Bioelectronics, Institute for Biomedical Engineering, Gloriastrasse 35, CH-8092 Zurich, Switzerland. grieshaber@biomed.ee.ethz.ch.
authors.forename: Dorothee
authors.initials: D
authors.lastname: Grieshaber
authors.affiliation: ETH Zurich, Laboratory of Biosensors and Bioelectronics, Institute for Biomedical Engineering, Gloriastrasse 35, CH-8092 Zurich, Switzerland. vdelange@student.ethz.ch.
authors.forename: Victoria
authors.initials: V
authors.lastname: De Lange
authors.affiliation: BioCure Inc., 2975 Gateway Dr. Suite 100, Norcross, 30071, GA USA. thirt@biocure.com.
authors.forename: Thomas
authors.initials: T
authors.lastname: Hirt
authors.affiliation: BioCure Inc., 2975 Gateway Dr. Suite 100, Norcross, 30071, GA USA. alu@biocure.com.
authors.forename: Zhihua
authors.initials: Z
authors.lastname: Lu
authors.affiliation: ETH Zurich, Laboratory of Biosensors and Bioelectronics, Institute for Biomedical Engineering, Gloriastrasse 35, CH-8092 Zurich, Switzerland. janos.voros@biomed.ee.ethz.ch.
authors.forename: Janos
authors.initials: J
authors.lastname: Vörös
keywords: High sensitivity; antigen detection; biosensor; lipid vesicles; quartz crystal microbalance