title: Genetically edited pigs lacking CD163 show no resistance following infection with the African swine fever virus isolate, Georgia 2007/1.
abstract: African swine fever is a highly contagious, often fatal disease of swine for which there is no vaccine or other curative treatment. The macrophage marker, CD163, is a putative receptor for African swine fever virus (ASFV). Pigs possessing a complete knockout of CD163 on macrophages were inoculated with Georgia 2007/1, a genotype 2 isolate. Knockout and wild type pen mates became infected and showed no differences in clinical signs, mortality, pathology or viremia. There was also no difference following in vitro infection of macrophages. The results do not rule out the possibility that other ASFV strains utilize CD163, but demonstrate that CD163 is not necessary for infection with the Georgia 2007/1 isolate. This work rules out a significant role for CD163 in ASFV infection and creates opportunities to focus on alternative receptors and entry mechanisms.
authors.affiliation: Department of Diagnostic Medicine and Pathobiology, College of Veterinary Medicine, Kansas State University, Manhattan, KS, USA.
authors.forename: Luca
authors.initials: L
authors.lastname: Popescu
authors.affiliation: Department of Diagnostic Medicine and Pathobiology, College of Veterinary Medicine, Kansas State University, Manhattan, KS, USA. Electronic address: nng5757@vet.k-state.edu.
authors.forename: Natasha N
authors.initials: NN
authors.lastname: Gaudreault
authors.affiliation: Division of Animal Science, College of Food Agriculture and Natural Resources, University of Missouri, Columbia, MO, USA.
authors.forename: Kristen M
authors.initials: KM
authors.lastname: Whitworth
authors.affiliation: Department of Diagnostic Medicine and Pathobiology, College of Veterinary Medicine, Kansas State University, Manhattan, KS, USA.
authors.forename: Maria V
authors.initials: MV
authors.lastname: Murgia
authors.affiliation: Department of Diagnostic Medicine and Pathobiology, College of Veterinary Medicine, Kansas State University, Manhattan, KS, USA.
authors.forename: Jerome C
authors.initials: JC
authors.lastname: Nietfeld
authors.affiliation: Genus, plc, DeForest, Wisconsin, USA.
authors.forename: Alan
authors.initials: A
authors.lastname: Mileham
authors.affiliation: Division of Animal Science, College of Food Agriculture and Natural Resources, University of Missouri, Columbia, MO, USA.
authors.forename: Melissa
authors.initials: M
authors.lastname: Samuel
authors.affiliation: Division of Animal Science, College of Food Agriculture and Natural Resources, University of Missouri, Columbia, MO, USA.
authors.forename: Kevin D
authors.initials: KD
authors.lastname: Wells
authors.affiliation: Division of Animal Science, College of Food Agriculture and Natural Resources, University of Missouri, Columbia, MO, USA.
authors.forename: Randall S
authors.initials: RS
authors.lastname: Prather
authors.affiliation: Department of Diagnostic Medicine and Pathobiology, College of Veterinary Medicine, Kansas State University, Manhattan, KS, USA.
authors.forename: Raymond R R
authors.initials: RRR
authors.lastname: Rowland
mesh_terms: D000357:African Swine Fever / Q000235:genetics* / Q000378:metabolism / Q000821:virology; D000358:African Swine Fever Virus / Q000235:genetics / Q000502:physiology*; D000818:Animals; D030801:Animals, Genetically Modified / Q000235:genetics / Q000378:metabolism* / Q000821:virology; D015703:Antigens, CD / Q000235:genetics; D015214:Antigens, Differentiation, Myelomonocytic / Q000235:genetics; D055786:Gene Knockout Techniques; D005845:Georgia; D008264:Macrophages / Q000378:metabolism / Q000821:virology; D011956:Receptors, Cell Surface / Q000172:deficiency* / Q000235:genetics; D011991:Receptors, Virus / Q000235:genetics / Q000378:metabolism; D013552:Swine / Q000235:genetics* / Q000378:metabolism / Q000821:virology; D000098902:CD163 Antigen
chemical_list: D015703:Antigens, CD; D015214:Antigens, Differentiation, Myelomonocytic; D000098902:CD163 Antigen; D011956:Receptors, Cell Surface; D011991:Receptors, Virus
keywords: African swine fever; CD163; Receptor; Virus