title: Label-Free Quantitation of Ribosomal Proteins from Bacillus subtilis for Antibiotic Research.
abstract: Current research is focusing on ribosome heterogeneity as a response to changing environmental conditions and stresses, such as antibiotic stress. Altered stoichiometry and composition of ribosomal proteins as well as association of additional protein factors are mechanisms for shaping the protein expression profile or hibernating ribosomes. Here, we present a method for the isolation of ribosomes to analyze antibiotic-induced changes in the composition of ribosomes in Bacillus subtilis or other bacteria. Ribosomes and associated proteins are isolated by ultracentrifugation and proteins are identified and quantified using label-free mass spectrometry.
authors.affiliation: Applied Microbiology, Ruhr-Universität Bochum, Universitätsstr. 150, Bochum, 44801, Germany. Sina.Langklotz@rub.de.
authors.forename: Sina
authors.initials: S
authors.lastname: Schäkermann
authors.affiliation: Applied Microbiology, Ruhr-Universität Bochum, Universitätsstr. 150, Bochum, 44801, Germany.
authors.forename: Pascal
authors.initials: P
authors.lastname: Prochnow
authors.affiliation: Applied Microbiology, Ruhr-Universität Bochum, Universitätsstr. 150, Bochum, 44801, Germany.
authors.forename: Julia E
authors.initials: JE
authors.lastname: Bandow
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001412:Bacillus subtilis / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*; D013058:Mass Spectrometry; D012269:Ribosomal Proteins / Q000378:metabolism*; D012270:Ribosomes / Q000378:metabolism; D013194:Staining and Labeling; D014357:Trypsin / Q000378:metabolism
chemical_list: D000900:Anti-Bacterial Agents; D012269:Ribosomal Proteins; D014357:Trypsin
keywords: Mass spectrometry; Proteomics; Ribosome heterogeneity; Stress