title: Influence of biological scaffold regulation on the proliferation of chondrocytes and the repair of articular cartilage.
abstract: OBJECTIVE
  To investigate the effects of hard tissue engineering scaffold (the material is β-TCP) with different micro-structures on the proliferation of chondrocytes, and the influence of its composite erythrocytes on the repair of articular cartilage defects.
  
  
  METHODS
  Rabbit cartilage cells were on β-TCP bioceramic scaffold with different micro-structures in vitro, the proliferation growth trend of chondrocytes within the scaffold was calculated, and a optimal micro-structure suitable for cartilage cell growth was determined. Composite chondrocytes were implanted into rabbit models of articular cartilage defects, and the repair situation was observed.
  
  
  RESULTS
  the bioceramic scaffold with an inner diameter of 120 μm and an aperture of 500-630 μm was suitable for the growth of cartilage cells. Scaffold loaded with second generation of cartilage cell suspension got a top histological score of 20.76±2.13, which was closely similar to that of normal cartilage.
  
  
  CONCLUSIONS
  When loaded with the second generation of cartilage cells, the β-TCP biological ceramic scaffold with a pore size of 500-630 μm, and an inner diameter of 120 μm, shows a best repairing effect on animal articular cartilage defects.
authors.affiliation: Department of Orthopaedics, Huashan Hospital of Fudan University Shanghai 200040, China.
authors.forename: Si-Qun
authors.initials: SQ
authors.lastname: Wang
authors.affiliation: Department of Orthopaedics, Huashan Hospital of Fudan University Shanghai 200040, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Xia
authors.affiliation: Department of Orthopaedics, Huashan Hospital of Fudan University Shanghai 200040, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Chen
authors.affiliation: Shanghai Bio-Lu Biomaterials Co. Ltd Shanghai 201114, China.
authors.forename: Jian-Xi
authors.initials: JX
authors.lastname: Lu
authors.affiliation: Department of Orthopaedics, Huashan Hospital of Fudan University Shanghai 200040, China.
authors.forename: Yi-Bing
authors.initials: YB
authors.lastname: Wei
authors.affiliation: Department of Orthopaedics, Huashan Hospital of Fudan University Shanghai 200040, China.
authors.forename: Fei-Yan
authors.initials: FY
authors.lastname: Chen
authors.affiliation: Department of Orthopaedics, Huashan Hospital of Fudan University Shanghai 200040, China.
authors.forename: Gang-Yong
authors.initials: GY
authors.lastname: Huang
authors.affiliation: Department of Orthopaedics, Huashan Hospital of Fudan University Shanghai 200040, China.
authors.forename: Jing-Sheng
authors.initials: JS
authors.lastname: Shi
authors.affiliation: Department of Orthopaedics, Huashan Hospital of Fudan University Shanghai 200040, China.
authors.forename: Yong-Lin
authors.initials: YL
authors.lastname: Yu
keywords: Cartilage; articular cartilage repair; biological ceramic; cartilage cell proliferation