title: Development and characterization of twelve microsatellite markers for Porphyra linearis Greville.
abstract: The genus Porphyra (and its sister genus Pyropia) contains important red algal species that are cultivated and/or harvested for human consumption, sustaining a billion-dollar aquaculture industry. A vast amount of research has been focused on species of this genus, including studies on genetics and genomics among other areas. Twelve novel microsatellite markers were developed here for Porphyra linearis. Markers were characterized using 32 individuals collected from four natural populations of P. linearis with total heterozygosity varying from 0.098 to 0.916. The number of alleles per locus ranged from 2 to 18. All markers showed cross amplification with Porphyra umbilicalis and/or Porphyra dioica. These polymorphic microsatellite markers are useful for investigating population genetic diversity and differentiation in P. linearis and may become useful for other genetic research on the reproductive biology of this important species.
authors.affiliation: Centro de Ciências do Mar, Laboratório Associado, (CCMAR-CIMAR), Universidade do Algarve, Campus de Gambelas, 8005-139, Faro, Portugal. evarela@ualg.pt.
authors.forename: Elena
authors.identifier: http://orcid.org/0000-0001-7262-3475
authors.initials: E
authors.lastname: Varela-Álvarez
authors.affiliation: Centro de Ciências do Mar, Laboratório Associado, (CCMAR-CIMAR), Universidade do Algarve, Campus de Gambelas, 8005-139, Faro, Portugal.
authors.forename: Cristina
authors.initials: C
authors.lastname: Paulino
authors.affiliation: Centro de Ciências do Mar, Laboratório Associado, (CCMAR-CIMAR), Universidade do Algarve, Campus de Gambelas, 8005-139, Faro, Portugal.
authors.forename: Ester A
authors.initials: EA
authors.lastname: Serrão
mesh_terms: D000483:Alleles; D014644:Genetic Variation; D005828:Genetics, Population; D018895:Microsatellite Repeats; D044664:Porphyra / Q000145:classification / Q000235:genetics*
keywords: Microsatellite markers; North Atlantic; Population structure; Porphyra; Red algae