title: MiR-5100 promotes osteogenic differentiation by targeting Tob2.
abstract: MicroRNAs have emerged as pivotal regulators in various physiological and pathological processes, including osteogenesis. Here we discuss the contribution of miR-5100 to osteoblast differentiation and mineralization. We found that miR-5100 was upregulated during osteoblast differentiation in ST2 and MC3T3-E1 cells. Next, we verified that miR-5100 can promote osteogenic differentiation with gain-of-function and loss-of-function experiments. Target prediction analysis and experimental validation demonstrated that Tob2, which acts as a negative regulator of osteogenesis, was negatively regulated by miR-5100. Furthermore, we confirmed that the important bone-related transcription factor osterix, which can be degraded by binding to Tob2, was influenced by miR-5100 during osteoblast differentiation. Collectively, our results revealed a new molecular mechanism that fine-tunes osteoblast differentiation through miR-5100/Tob2/osterix networks.
authors.affiliation: Shandong University of Traditional Chinese Medicine, Jinan, Shandong, China.|Shandong Medical Biotechnological Center, Key Laboratory for Biotech Drugs of the Ministry of Health, Shandong Academy of Medical Sciences, Jinan, China.
authors.forename: Huaxin
authors.initials: H
authors.lastname: Wang
authors.affiliation: Shandong Medical Biotechnological Center, Key Laboratory for Biotech Drugs of the Ministry of Health, Shandong Academy of Medical Sciences, Jinan, China.
authors.forename: Yazhou
authors.initials: Y
authors.lastname: Cui
authors.affiliation: Shandong Medical Biotechnological Center, Key Laboratory for Biotech Drugs of the Ministry of Health, Shandong Academy of Medical Sciences, Jinan, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Luan
authors.affiliation: Shandong Medical Biotechnological Center, Key Laboratory for Biotech Drugs of the Ministry of Health, Shandong Academy of Medical Sciences, Jinan, China.
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Zhou
authors.affiliation: Shandong Medical Biotechnological Center, Key Laboratory for Biotech Drugs of the Ministry of Health, Shandong Academy of Medical Sciences, Jinan, China.
authors.forename: Chengzhi
authors.initials: C
authors.lastname: Li
authors.affiliation: Shandong Medical Biotechnological Center, Key Laboratory for Biotech Drugs of the Ministry of Health, Shandong Academy of Medical Sciences, Jinan, China.
authors.forename: Haiying
authors.initials: H
authors.lastname: Li
authors.affiliation: Shandong Medical Biotechnological Center, Key Laboratory for Biotech Drugs of the Ministry of Health, Shandong Academy of Medical Sciences, Jinan, China.
authors.forename: Liang
authors.initials: L
authors.lastname: Shi
authors.affiliation: Shandong Medical Biotechnological Center, Key Laboratory for Biotech Drugs of the Ministry of Health, Shandong Academy of Medical Sciences, Jinan, China. jxhan88@126.com.
authors.forename: Jinxiang
authors.initials: J
authors.lastname: Han
mesh_terms: D000818:Animals; D001483:Base Sequence; D018797:Cell Cycle Proteins / Q000235:genetics* / Q000378:metabolism; D002454:Cell Differentiation / Q000235:genetics*; D002460:Cell Line; D005786:Gene Expression Regulation; D051379:Mice; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D010006:Osteoblasts / Q000166:cytology / Q000378:metabolism; D010012:Osteogenesis / Q000235:genetics*; D000076182:Sp7 Transcription Factor / Q000235:genetics / Q000378:metabolism
chemical_list: D018797:Cell Cycle Proteins; D035683:MicroRNAs; C000626848:Mirn5100 microRNA, mouse; D000076182:Sp7 Transcription Factor; C479466:Sp7 protein, mouse; C485732:Tob2 protein, mouse
keywords: Mineralization; Osteoblast differentiation; Tob2; miR-5100