title: Neuropeptide Y receptor 1 is expressed by B and T lymphocytes and mast cells in infantile haemangiomas.
abstract: OBJECTIVE
  We investigated the expression of neuropeptide Y (NPY), NPY receptor 1 (NPYR1) and NPY receptor 2 (NPYR2) in infantile haemangiomas (IHs).
  
  
  METHODS
  Immunohistochemical (IHC) staining was performed on proliferating IHs from six patients aged 4-13 (mean 8.7) months and involuted IHs from six patients aged 5-59 (mean 18.7) years, for the expression of NPY, NPYR1 and NPYR2. Protein and messenger ribonucleic acid expression corresponding to these proteins was investigated by Western blotting and NanoString analysis, respectively.
  
  
  RESULTS
  IHC staining, Western blotting and NanoString analysis demonstrated the presence of NPYR1, but not NPYR2, within proliferating and involuted IHs. IHC staining showed NPYR1 was expressed by B and T lymphocytes expressing CD45 and mast cells expressing tryptase. IHC staining demonstrated the presence of NPY on the NPYR1+cells, but it was not detected by Western blotting or NanoString analysis.
  
  
  CONCLUSIONS
  NPYR1, but not NPYR2, was present in IHs. The localisation of NPYR1 to B and T lymphocytes and mast cells suggests its role in the biology of IHs. The demonstration of NPY on the NPYR1+cells, without active transcription, suggests that NPY was not being produced within IHs.
authors.affiliation: Gillies McIndoe Research Institute, Wellington, New Zealand.
authors.forename: Elysia M S
authors.initials: EM
authors.lastname: Tan
authors.affiliation: Gillies McIndoe Research Institute, Wellington, New Zealand.
authors.forename: Max G
authors.initials: MG
authors.lastname: Blackwell
authors.affiliation: Gillies McIndoe Research Institute, Wellington, New Zealand.
authors.forename: Jonathan C
authors.initials: JC
authors.lastname: Dunne
authors.affiliation: Gillies McIndoe Research Institute, Wellington, New Zealand.|University of Auckland, Auckland, New Zealand.
authors.forename: Reginald
authors.initials: R
authors.lastname: Marsh
authors.affiliation: Gillies McIndoe Research Institute, Wellington, New Zealand.|Centre for the Study and Treatment of Vascular Birthmarks, Wellington Regional Plastic, Maxillofacial and Burns Unit, Hutt Hospital, Wellington, New Zealand.
authors.forename: Swee T
authors.initials: ST
authors.lastname: Tan
authors.affiliation: Gillies McIndoe Research Institute, Wellington, New Zealand.
authors.forename: Tinte
authors.initials: T
authors.lastname: Itinteang
mesh_terms: D000293:Adolescent; D000328:Adult; D001402:B-Lymphocytes / Q000378:metabolism*; D015153:Blotting, Western; D002452:Cell Count; D002648:Child; D002675:Child, Preschool; D015870:Gene Expression; D006391:Hemangioma / Q000276:immunology* / Q000378:metabolism / Q000473:pathology; D006801:Humans; D007150:Immunohistochemistry; D008407:Mast Cells / Q000378:metabolism*; D017476:Receptors, Neuropeptide Y / Q000378:metabolism*; D013601:T-Lymphocytes / Q000378:metabolism*; D055815:Young Adult
chemical_list: D017476:Receptors, Neuropeptide Y; C097212:neuropeptide Y-Y1 receptor; C095889:neuropeptide Y2 receptor
keywords: Angiogenesis; Infantile haemangioma; Neural crest; Neuropeptide Y; Receptors