title: Desulfonatronospira sulfatiphila sp. nov., and Desulfitispora elongata sp. nov., two novel haloalkaliphilic sulfidogenic bacteria from soda lakes.
abstract: Two novel haloalkaliphilic bacteria with dissimilatory sulfidogenic metabolism were recovered from syntrophic associations obtained from anaerobic sediments of hypersaline soda lakes in Kulunda Steppe (Altai, Russia). Strain ASO3-2T was a member of a sulfidogenic syntrophic association oxidizing acetate at extremely haloalkaline conditions, and was isolated in pure culture using formate as electron donor and sulfate as electron acceptor. It was identified as representing a novel member of the genus Desulfonatronospira within the Deltaproteobacteria. In contrast to the two known species of this genus, the novel isolate was able to grow with formate as electron donor and sulfate, as well as with sulfite, as electron acceptor. Strain Acr1T was a minor component in a soda lake syntrophic association converting benzoate to methane and acetate. It became dominant in a subculture fed with crotonate. While growing on crotonate, strain Acr1T formed unusually long cells filled with polyhydroxyalkanoate-like granules. Its metabolism was limited to fermentation of crotonate and pyruvate and the ability to utilize thiosulfate and sulfur/polysulfide as electron acceptor. Strain Acr1T was identified as representing a novel member of the genus Desulfitispora in the class Clostridia. Both isolates were obligately haloalkaliphilic with extreme salt tolerance. On the basis of phenotypic and phylogenetic analyses, the novel sulfidogenic isolates from soda lakes are proposed to represent two novel species: Desulfonatronospira sulfatiphila sp. nov. (ASO3-2T=DSM 100427=UNIQEM U993T) and Desulfitispora elongata sp. nov. (Acr1T=DSM 29990=UNIQEM U994T).
authors.affiliation: Winogradsky Institute of Microbiology, Research Centre of Biotechnology, Russian Academy of Sciences, Leninskii Avenue 33/2, Moscow 119071, Russia.|Department of Biotechnology, Delft University of Technology, Van der Maasweg 9, 2629 HZ Delft, The Netherlands.
authors.forename: Dimitry Y
authors.initials: DY
authors.lastname: Sorokin
authors.affiliation: Winogradsky Institute of Microbiology, Research Centre of Biotechnology, Russian Academy of Sciences, Leninskii Avenue 33/2, Moscow 119071, Russia.
authors.forename: Nikolai A
authors.initials: NA
authors.lastname: Chernyh
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D020564:Deltaproteobacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D005561:Formates / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D060106:Lakes / Q000382:microbiology*; D010459:Peptococcaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012426:Russia; D054712:Salinity; D017422:Sequence Analysis, DNA; D013431:Sulfates / Q000737:chemistry; D013447:Sulfites / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005561:Formates; D012336:RNA, Ribosomal, 16S; D013431:Sulfates; D013447:Sulfites; C030544:formic acid