title: EGb-761 Attenuates the Anti-proliferative Activity of Fluoride via DDK1 in PC-12 Cells.
abstract: EGb-761 is commonly used as a treatment for ischemic brain injury, neurodegenerative diseases and some types of tumors (Christen and Maixent, in Cell Mol Biol 48(6):601-611, 2002). However, it is unclear whether EGb-761 affects the proliferation of cells exposed to fluoride. In this study, the proliferation and apoptosis of PC-12 cells exposed to fluoride were investigated and EGb-761 was used to protect PC-12 cells against the effects of fluoride. We found that the canonical Wnt signaling pathway was involved in the anti-proliferation of PC-12 cells exposed to fluoride. Furthermore, the results also showed that EGb-761 could attenuate the anti-proliferative activity of fluoride via DDK1 in PC-12 cells. This study may provide a new method for protecting against the inhibition of cell proliferation induced by fluoride.
authors.affiliation: Department of Neurology and Suzhou Clinical Research Center of Neurological Disease, The Second Affiliated Hospital of Soochow University, Suzhou, 215004, China.|Xuzhou Medical University Affiliated Xuzhou Oriental Hospital, No. 379 Tongshan Road, Xuzhou, 221000, Jiangsu Province, China.
authors.forename: Cai-Yi
authors.initials: CY
authors.lastname: Zhang
authors.affiliation: Department of Neurology, Xuzhou Medical University Affiliated Hospital, No. 99 Huaihai Road, Xuzhou, 221000, Jiangsu Province, China.
authors.forename: Rui
authors.initials: R
authors.lastname: Chen
authors.affiliation: Department of Neurology and Suzhou Clinical Research Center of Neurological Disease, The Second Affiliated Hospital of Soochow University, Suzhou, 215004, China.|Institute of Neuroscience, Soochow University, Suzhou, 215123, China.
authors.forename: Fen
authors.initials: F
authors.lastname: Wang
authors.affiliation: Department of Neurology, Affiliated Yantai Yuhuangding Hospital of Qingdao University Medical College, Yantai, 264000, China.
authors.forename: Chao
authors.initials: C
authors.lastname: Ren
authors.affiliation: Xuzhou Medical University Affiliated Xuzhou Oriental Hospital, No. 379 Tongshan Road, Xuzhou, 221000, Jiangsu Province, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Zhang
authors.affiliation: Department of Neurology and Suzhou Clinical Research Center of Neurological Disease, The Second Affiliated Hospital of Soochow University, Suzhou, 215004, China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Li
authors.affiliation: Department of Neurology, Xuzhou Medical University Affiliated Hospital, No. 99 Huaihai Road, Xuzhou, 221000, Jiangsu Province, China.
authors.forename: Hui-Hua
authors.initials: HH
authors.lastname: Li
authors.affiliation: Institute of Neuroscience, Soochow University, Suzhou, 215123, China.
authors.forename: Ke-Tai
authors.initials: KT
authors.lastname: Guo
authors.affiliation: Department of Neurology, Xuzhou Medical University Affiliated Hospital, No. 99 Huaihai Road, Xuzhou, 221000, Jiangsu Province, China.
authors.forename: De-Qin
authors.initials: DQ
authors.lastname: Geng
authors.affiliation: Department of Neurology and Suzhou Clinical Research Center of Neurological Disease, The Second Affiliated Hospital of Soochow University, Suzhou, 215004, China. liuchunfeng@suda.edu.cn.|Institute of Neuroscience, Soochow University, Suzhou, 215123, China. liuchunfeng@suda.edu.cn.
authors.forename: Chun-Feng
authors.initials: CF
authors.lastname: Liu
mesh_terms: D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects* / Q000502:physiology; D004305:Dose-Response Relationship, Drug; D005090:Exodeoxyribonucleases / Q000096:biosynthesis*; D020441:Ginkgo biloba; D016716:PC12 Cells; D010936:Plant Extracts / Q000494:pharmacology*; D051381:Rats; D012969:Sodium Fluoride / Q000633:toxicity*; D000096983:Ginkgo Extract
chemical_list: D005090:Exodeoxyribonucleases; D010936:Plant Extracts; D012969:Sodium Fluoride; C063170:Ginkgo biloba extract; D000096983:Ginkgo Extract; C579164:MGME1 protein, human
keywords: DKK1; EGb-761; Proliferation; Wnt singaling pathway