title: The effect of cumulus cells on domestic cat (Felis catus) oocytes during in vitro maturation and fertilization.
abstract: The aim of this study was to evaluate the effect of co-culture of denuded oocytes with cumulus cells (CC) or cumulus-oocyte complexes (COCs) on in vitro maturation (IVM) and in vitro fertilization (IVF). Immature oocytes were collected from ovaries of domestic cats following a routine ovariectomy. Oocytes were matured in vitro for 24 hr within four groups: (i) denuded oocytes (DO), (ii) DO co-cultured with CC, (iii) DO co-cultured with COC and (iv) COC as a control group. In further experiments, COCs were matured in vitro for 24 hr, and then, oocytes were randomly divided into four groups as previously described and fertilized in vitro. Embryos were cultured for up to 7 days. At the end of each experiment, oocytes/embryos were stained with Hoechst 33342 solution and observed under an inverted fluorescence microscope. The results of oocyte maturation showed that their meiotic competence decreased significantly in all experimental groups, compared to the control group. The maturation rates were approximately 45%, 24%, 43% and 76% in experiment 1, and 21%, 14%, 33% and 50% in experiment 2 in groups (i), (ii), (iii) and (iv), respectively. Examination of in vitro fertilization revealed that embryos developed up to the morula stage in all experimental groups. DO and oocytes cultured with COC during fertilization showed a lower cleavage rate-36% and 25% as opposed to those co-cultured with loose CC and the control group-43% and 42%, respectively. Results of this study indicate that cumulus cells connected with an oocyte into a cumulus-oocyte complex are irreplaceable for the maturation of domestic cat oocyte, but that the addition of loose CC may be beneficial for IVF.
authors.affiliation: University Center of Veterinary Medicine JU-AU, Center of Experimental and Innovative Medicine, University of Agriculture in Krakow, Krakow, Poland.
authors.forename: N
authors.initials: N
authors.lastname: Sowińska
authors.affiliation: University Center of Veterinary Medicine JU-AU, Center of Experimental and Innovative Medicine, University of Agriculture in Krakow, Krakow, Poland.
authors.forename: K
authors.initials: K
authors.lastname: Frankowska
authors.affiliation: University Center of Veterinary Medicine JU-AU, Center of Experimental and Innovative Medicine, University of Agriculture in Krakow, Krakow, Poland.
authors.forename: A
authors.initials: A
authors.lastname: Filipczyk
authors.affiliation: University Center of Veterinary Medicine JU-AU, Center of Experimental and Innovative Medicine, University of Agriculture in Krakow, Krakow, Poland.
authors.forename: A
authors.initials: A
authors.lastname: Adamaszek
authors.affiliation: University Center of Veterinary Medicine JU-AU, Center of Experimental and Innovative Medicine, University of Agriculture in Krakow, Krakow, Poland.
authors.forename: K
authors.initials: K
authors.lastname: Nalik
authors.affiliation: University Center of Veterinary Medicine JU-AU, Center of Experimental and Innovative Medicine, University of Agriculture in Krakow, Krakow, Poland.
authors.forename: K
authors.initials: K
authors.lastname: Fic
authors.affiliation: University Center of Veterinary Medicine JU-AU, Center of Experimental and Innovative Medicine, University of Agriculture in Krakow, Krakow, Poland.
authors.forename: A
authors.initials: A
authors.lastname: Pietsch-Fulbiszewska
mesh_terms: D000818:Animals; D002415:Cats / Q000502:physiology; D018920:Coculture Techniques; D003470:Culture Media / Q000494:pharmacology; D054885:Cumulus Cells / Q000166:cytology*; D005260:Female; D005307:Fertilization in Vitro / Q000662:veterinary*; D059471:In Vitro Oocyte Maturation Techniques / Q000662:veterinary*; D008297:Male; D009028:Morula / Q000502:physiology; D009865:Oocytes / Q000166:cytology*; D009866:Oogenesis / Q000502:physiology*; D011897:Random Allocation; D013094:Spermatozoa / Q000502:physiology
chemical_list: D003470:Culture Media