title: Variations in endothelin receptor B subtype 2 (EDNRB2) coding sequences and mRNA expression levels in 4 Muscovy duck plumage colour phenotypes.
abstract: 1. Endothelin receptor B subtype 2 (EDNRB2) is a paralog of EDNRB, which encodes a 7-transmembrane G-protein coupled receptor. Previous studies reported that EDNRB was essential for melanoblast migration in mammals and ducks. 2. Muscovy ducks have different plumage colour phenotypes. Variations in EDNRB2 coding sequences (CDSs) and mRNA expression levels were investigated in 4 different Muscovy duck plumage colour phenotypes, including black, black mutant, silver and white head. 3. The EDNRB2 gene from Muscovy duck was cloned; it had a length of 6435 bp and encoded 437 amino acids. The coding region was screened and potential single nucleotide polymorphisms were identified. Eight mutations were obtained, including one missense variant (c.64C > T) and 7 synonymous substitutions. The substitutions were associated with plumage colour phenotypes. 4. The EDNRB2 mRNA expression levels were compared between feather pulp from black birds and black mutant birds. The results indicated that EDNRB2 transcripts in feather pulp were significantly higher in black feathers than in white feathers. 5. The results determined the variation of EDNRB2 CDS and mRNA expression in Muscovy ducks of various plumage colours.
authors.affiliation: a Key Laboratory of Animal Genetics and Breeding and Molecular Design of Jiangsu Province , Yangzhou University , Yangzhou , People's Republic of China.
authors.forename: N
authors.initials: N
authors.lastname: Wu
authors.affiliation: b National Waterfowl Germplasm Resource Pool , Taizhou , People's Republic of China.
authors.forename: H
authors.initials: H
authors.lastname: Qin
authors.affiliation: a Key Laboratory of Animal Genetics and Breeding and Molecular Design of Jiangsu Province , Yangzhou University , Yangzhou , People's Republic of China.
authors.forename: M
authors.initials: M
authors.lastname: Wang
authors.affiliation: b National Waterfowl Germplasm Resource Pool , Taizhou , People's Republic of China.
authors.forename: Y
authors.initials: Y
authors.lastname: Bian
authors.affiliation: b National Waterfowl Germplasm Resource Pool , Taizhou , People's Republic of China.
authors.forename: B
authors.initials: B
authors.lastname: Dong
authors.affiliation: b National Waterfowl Germplasm Resource Pool , Taizhou , People's Republic of China.
authors.forename: G
authors.initials: G
authors.lastname: Sun
authors.affiliation: a Key Laboratory of Animal Genetics and Breeding and Molecular Design of Jiangsu Province , Yangzhou University , Yangzhou , People's Republic of China.
authors.forename: W
authors.initials: W
authors.lastname: Zhao
authors.affiliation: a Key Laboratory of Animal Genetics and Breeding and Molecular Design of Jiangsu Province , Yangzhou University , Yangzhou , People's Republic of China.
authors.forename: G
authors.initials: G
authors.lastname: Chang
authors.affiliation: a Key Laboratory of Animal Genetics and Breeding and Molecular Design of Jiangsu Province , Yangzhou University , Yangzhou , People's Republic of China.
authors.forename: Q
authors.initials: Q
authors.lastname: Xu
authors.affiliation: a Key Laboratory of Animal Genetics and Breeding and Molecular Design of Jiangsu Province , Yangzhou University , Yangzhou , People's Republic of China.
authors.forename: G
authors.initials: G
authors.lastname: Chen
mesh_terms: D000818:Animals; D030161:Avian Proteins / Q000235:genetics* / Q000378:metabolism; D003116:Color; D004372:Ducks / Q000235:genetics / Q000502:physiology*; D005241:Feathers / Q000737:chemistry*; D010641:Phenotype; D010802:Phylogeny; D010858:Pigmentation / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D044023:Receptor, Endothelin B / Q000235:genetics* / Q000378:metabolism; D017422:Sequence Analysis, DNA
chemical_list: D030161:Avian Proteins; D012333:RNA, Messenger; D044023:Receptor, Endothelin B
keywords: EDNRB2; Muscovy duck; polymorphism; quantitative real-time PCR