title: Serum miR-300 as a diagnostic and prognostic biomarker in osteosarcoma.
abstract: In order to determine whether microRNA (miR)-300 is a diagnostic and prognostic biomarker in osteosarcoma, the miR-300 levels in serum of 114 osteosarcoma patients and 114 healthy controls were compared, followed by serum analysis of the differences between the pre-operative and post-operative sera of these osteosarcoma patients. It was observed that the concentration levels of miR-300 in the serum of osteosarcoma patients was significantly higher than those in the serum of healthy controls (P<0.01). Furthermore, the concentration levels of miR-300 in the post-operative serum were significantly reduced when compared with the pre-operative serum levels (P<0.001). High miR-300 levels in serum correlated significantly with clinical stage, distant metastasis and poor survival of osteosarcoma patients. Notably, serum miR-300 was an independent prognostic marker for osteosarcoma. In conclusion, our results suggested that serum miR-300 may be a potential and useful noninvasive biomarker for the early detection of osteosarcoma.
authors.affiliation: Department of Orthopedics, Affiliated Hospital of Qingdao University, Qingdao, Shandong 266000, P.R. China; Department of Orthopedics, 401 Hospital of PLA, Qingdao, Shandong 266071, P.R. China.
authors.forename: Jian-Dong
authors.initials: JD
authors.lastname: Liu
authors.affiliation: Department of General Surgery, 401 Hospital of PLA, Qingdao, Shandong 266071, P.R. China.
authors.forename: Qun
authors.initials: Q
authors.lastname: Xin
authors.affiliation: Department of Orthopedics, 401 Hospital of PLA, Qingdao, Shandong 266071, P.R. China.
authors.forename: Chun-Sheng
authors.initials: CS
authors.lastname: Tao
authors.affiliation: Department of Orthopedics, 401 Hospital of PLA, Qingdao, Shandong 266071, P.R. China.
authors.forename: Pei-Feng
authors.initials: PF
authors.lastname: Sun
authors.affiliation: Department of Orthopedics, 401 Hospital of PLA, Qingdao, Shandong 266071, P.R. China.
authors.forename: Peng
authors.initials: P
authors.lastname: Xu
authors.affiliation: Department of Orthopedics, 401 Hospital of PLA, Qingdao, Shandong 266071, P.R. China.
authors.forename: Bing
authors.initials: B
authors.lastname: Wu
authors.affiliation: Department of Orthopedics, Affiliated Hospital of Qingdao University, Qingdao, Shandong 266000, P.R. China; Department of Orthopedics, 401 Hospital of PLA, Qingdao, Shandong 266071, P.R. China.
authors.forename: Liang
authors.initials: L
authors.lastname: Qu
authors.affiliation: Department of Orthopedics, Affiliated Hospital of Qingdao University, Qingdao, Shandong 266000, P.R. China.
authors.forename: Shu-Zhong
authors.initials: SZ
authors.lastname: Li
keywords: diagnostic; osteosarcoma; prognosis; serum miR-300