title: A DII Domain-Based Auxin Reporter Uncovers Low Auxin Signaling during Telophase and Early G1.
abstract: A sensitive and dynamically responsive auxin signaling reporter based on the DII domain of the INDOLE-3-ACETIC ACID28 (IAA28, DII) protein from Arabidopsis (Arabidopsis thaliana) was modified for use in maize (Zea mays). The DII domain was fused to a yellow fluorescent protein and a nuclear localization sequence to simplify quantitative nuclear fluorescence signal. DII degradation dynamics provide an estimate of input signal into the auxin signaling pathway that is influenced by both auxin accumulation and F-box coreceptor concentration. In maize, the DII-based marker responded rapidly and in a dose-dependent manner to exogenous auxin via proteasome-mediated degradation. Low levels of DII-specific fluorescence corresponding to high endogenous auxin signaling occurred near vasculature tissue and the outer layer and glume primordia of spikelet pair meristems and floral meristems, respectively. In addition, high DII levels were observed in cells during telophase and early G1, suggesting that low auxin signaling at these stages may be important for cell cycle progression.
authors.affiliation: Center for Plant Cell Biology (CEPCEB), Institute for Integrative Genome Biology, Department of Botany and Plant Sciences (R.M., L.Z.A., C.G.R.).|MARC U-STAR Program (L.Z.A.), and.|Biochemistry and Molecular Biology Graduate Program (T.B.), University of California, Riverside, California 92521; and.|Department of Molecular Biology, University of Wyoming, Laramie, Wyoming 82071 (A.L., A.W.S.).
authors.forename: Ricardo
authors.identifier: 0000-0003-1506-4683
authors.initials: R
authors.lastname: Mir
authors.affiliation: Center for Plant Cell Biology (CEPCEB), Institute for Integrative Genome Biology, Department of Botany and Plant Sciences (R.M., L.Z.A., C.G.R.).|MARC U-STAR Program (L.Z.A.), and.|Biochemistry and Molecular Biology Graduate Program (T.B.), University of California, Riverside, California 92521; and.|Department of Molecular Biology, University of Wyoming, Laramie, Wyoming 82071 (A.L., A.W.S.).
authors.forename: Leslie Z
authors.identifier: 0000-0002-4751-6974
authors.initials: LZ
authors.lastname: Aranda
authors.affiliation: Center for Plant Cell Biology (CEPCEB), Institute for Integrative Genome Biology, Department of Botany and Plant Sciences (R.M., L.Z.A., C.G.R.).|MARC U-STAR Program (L.Z.A.), and.|Biochemistry and Molecular Biology Graduate Program (T.B.), University of California, Riverside, California 92521; and.|Department of Molecular Biology, University of Wyoming, Laramie, Wyoming 82071 (A.L., A.W.S.).
authors.forename: Tiffany
authors.identifier: 0000-0002-6233-3033
authors.initials: T
authors.lastname: Biaocchi
authors.affiliation: Center for Plant Cell Biology (CEPCEB), Institute for Integrative Genome Biology, Department of Botany and Plant Sciences (R.M., L.Z.A., C.G.R.).|MARC U-STAR Program (L.Z.A.), and.|Biochemistry and Molecular Biology Graduate Program (T.B.), University of California, Riverside, California 92521; and.|Department of Molecular Biology, University of Wyoming, Laramie, Wyoming 82071 (A.L., A.W.S.).
authors.forename: Anding
authors.identifier: 0000-0002-2603-8854
authors.initials: A
authors.lastname: Luo
authors.affiliation: Center for Plant Cell Biology (CEPCEB), Institute for Integrative Genome Biology, Department of Botany and Plant Sciences (R.M., L.Z.A., C.G.R.).|MARC U-STAR Program (L.Z.A.), and.|Biochemistry and Molecular Biology Graduate Program (T.B.), University of California, Riverside, California 92521; and.|Department of Molecular Biology, University of Wyoming, Laramie, Wyoming 82071 (A.L., A.W.S.).
authors.forename: Anne W
authors.identifier: 0000-0001-7282-4189
authors.initials: AW
authors.lastname: Sylvester
authors.affiliation: Center for Plant Cell Biology (CEPCEB), Institute for Integrative Genome Biology, Department of Botany and Plant Sciences (R.M., L.Z.A., C.G.R.), carolyn.rasmussen@ucr.edu.|MARC U-STAR Program (L.Z.A.), and carolyn.rasmussen@ucr.edu.|Biochemistry and Molecular Biology Graduate Program (T.B.), University of California, Riverside, California 92521; and carolyn.rasmussen@ucr.edu.|Department of Molecular Biology, University of Wyoming, Laramie, Wyoming 82071 (A.L., A.W.S.) carolyn.rasmussen@ucr.edu.
authors.forename: Carolyn G
authors.identifier: 0000-0002-4354-6295
authors.initials: CG
authors.lastname: Rasmussen
mesh_terms: D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D016193:G1 Phase / Q000502:physiology; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D007210:Indoleacetic Acids / Q000378:metabolism* / Q000494:pharmacology; D018519:Meristem / Q000235:genetics / Q000378:metabolism; D030821:Plants, Genetically Modified; D000072417:Protein Domains; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction; D013692:Telophase / Q000502:physiology*; D059008:Time-Lapse Imaging; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D003313:Zea mays / Q000166:cytology* / Q000187:drug effects / Q000235:genetics / Q000378:metabolism
chemical_list: D029681:Arabidopsis Proteins; C530977:IAA28 protein, Arabidopsis; D007210:Indoleacetic Acids; D011994:Recombinant Proteins; D014157:Transcription Factors; D049452:Green Fluorescent Proteins; C030737:indoleacetic acid