title: Research on the Relationships between Endogenous Biomarkers and Exogenous Toxic Substances of Acute Toxicity in Radix Aconiti.
abstract: Radix Aconiti, a classic traditional Chinese medicine (TCM), has been widely used throughout China for disease treatment due to its various pharmacological activities, such as anti-inflammatory, cardiotonic, and analgesic effects. However, improper use ofRadix Aconitioften generated severe acute toxicity. Currently, research on the toxic substances ofRadix Aconitiis not rare. In our previous study, acute toxic biomarkers ofRadix Aconitihave been found. However, few studies were available to find the relationships between these endogenous biomarkers and exogenous toxic substances. Therefore, in this study, toxic substances ofRadix Aconitihave been found using UPLC-Q-TOF-MS technology. Then, we used biochemical indicators as a bridge to find the relationships between biomarkers and toxic substances ofRadix Aconitithrough Pearson correlation analysis and canonical correlation analysis (CCA). Finally, the CCA results showed that LysoPC(22:5) is related to 14-acetyl-talatisamine, mesaconitine, talatisamine and deoxyaconitine in varying degrees; l-acetylcarnitine is negatively correlated with deoxyaconitine and demethyl-14-acetylkaracoline; shikimic acid has a good correlation with karacoline, demethyl-14-acetylkaracoline and deoxyaconitine; and valine is correlated with talatisamine and deoxyaconitine. Research on these relationships provides an innovative way to interpret the toxic mechanism of traditional Chinese medicine, and plays a positive role in the overall study of TCM toxicity.
authors.affiliation: Tianjin State Key Laboratory of Modern Chinese Medicine, School of Traditional Chinese Materia Medica, Tianjin University of Traditional Chinese Medicine, 312 Anshan West Road, Tianjin 300193, China. zhnwork09@163.com.
authors.forename: Haonan
authors.initials: H
authors.lastname: Zhou
authors.affiliation: Tianjin State Key Laboratory of Modern Chinese Medicine, School of Traditional Chinese Materia Medica, Tianjin University of Traditional Chinese Medicine, 312 Anshan West Road, Tianjin 300193, China. 13752586596@163.com.
authors.forename: Pengjie
authors.initials: P
authors.lastname: Zhang
authors.affiliation: Tianjin State Key Laboratory of Modern Chinese Medicine, School of Traditional Chinese Materia Medica, Tianjin University of Traditional Chinese Medicine, 312 Anshan West Road, Tianjin 300193, China. hzgssh@163.com.
authors.forename: Zhiguo
authors.initials: Z
authors.lastname: Hou
authors.affiliation: Tianjin State Key Laboratory of Modern Chinese Medicine, School of Traditional Chinese Materia Medica, Tianjin University of Traditional Chinese Medicine, 312 Anshan West Road, Tianjin 300193, China. 18600893359@163.com.
authors.forename: Jiabin
authors.initials: J
authors.lastname: Xie
authors.affiliation: Tianjin State Key Laboratory of Modern Chinese Medicine, School of Traditional Chinese Materia Medica, Tianjin University of Traditional Chinese Medicine, 312 Anshan West Road, Tianjin 300193, China. wangyuming226@163.com.
authors.forename: Yuming
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Tianjin State Key Laboratory of Modern Chinese Medicine, School of Traditional Chinese Materia Medica, Tianjin University of Traditional Chinese Medicine, 312 Anshan West Road, Tianjin 300193, China. yang3023008@163.com.
authors.forename: Bin
authors.initials: B
authors.lastname: Yang
authors.affiliation: Tianjin State Key Laboratory of Modern Chinese Medicine, School of Traditional Chinese Materia Medica, Tianjin University of Traditional Chinese Medicine, 312 Anshan West Road, Tianjin 300193, China. xyytjutcm@163.com.
authors.forename: Yanyan
authors.initials: Y
authors.lastname: Xu
authors.affiliation: Tianjin State Key Laboratory of Modern Chinese Medicine, School of Traditional Chinese Materia Medica, Tianjin University of Traditional Chinese Medicine, 312 Anshan West Road, Tianjin 300193, China. yaowufenxi001@sina.com.
authors.forename: Yubo
authors.initials: Y
authors.lastname: Li
mesh_terms: D031841:Aconitum / Q000737:chemistry*; D000818:Animals; D015415:Biomarkers / Q000097:blood*; D004365:Drugs, Chinese Herbal / Q000633:toxicity*; D006321:Heart / Q000187:drug effects; D007668:Kidney / Q000187:drug effects / Q000473:pathology; D008099:Liver / Q000187:drug effects / Q000473:pathology; D051381:Rats
chemical_list: D015415:Biomarkers; D004365:Drugs, Chinese Herbal
keywords: Radix Aconiti; acute toxicity; biomarkers; corresponding relationships; toxic substances