title: Low expression of angiotensinogen and dipeptidyl peptidase 1 in saliva of patients with proliferative verrucous leukoplakia.
abstract: OBJECTIVE
  To elucidate the profile of the salivary proteome.
  
  
  METHODS
  Unstimulated whole mouth saliva was collected from 30 volunteers [15 proliferative verrucous leukoplakia (PVL) patients and 15 controls] and proteins were submitted for mass spectrometry-based proteomics using the discovery approach, followed by analyses of variance and logistic regression tests.
  
  
  RESULTS
  A total of two hundred and eighty-three proteins were confidently identified in saliva. By combining two low abundance proteins from the PVL group, angiotensinogen (AGT) and dipeptidyl peptidase 1 (DPP1), a model for group differentiation was built with a concordance index of 94.2%, identifying both proteins as potential etiologic biomarkers for PVL.
  
  
  CONCLUSIONS
  This study suggests that both AGT and DPP1 may be involved in developmental mechanisms of PVL.
authors.affiliation: Isadora Luana Flores, Alan Roger Santos-Silva, Ricardo Della Coletta, Marcio Ajudarte Lopes, Oral Diagnosis Department, Piracicaba Dental School, University of Campinas (UNICAMP), Piracicaba, São Paulo 13414-903, Brazil.
authors.forename: Isadora Luana
authors.initials: IL
authors.lastname: Flores
authors.affiliation: Isadora Luana Flores, Alan Roger Santos-Silva, Ricardo Della Coletta, Marcio Ajudarte Lopes, Oral Diagnosis Department, Piracicaba Dental School, University of Campinas (UNICAMP), Piracicaba, São Paulo 13414-903, Brazil.
authors.forename: Alan Roger
authors.initials: AR
authors.lastname: Santos-Silva
authors.affiliation: Isadora Luana Flores, Alan Roger Santos-Silva, Ricardo Della Coletta, Marcio Ajudarte Lopes, Oral Diagnosis Department, Piracicaba Dental School, University of Campinas (UNICAMP), Piracicaba, São Paulo 13414-903, Brazil.
authors.forename: Ricardo Della
authors.initials: RD
authors.lastname: Coletta
authors.affiliation: Isadora Luana Flores, Alan Roger Santos-Silva, Ricardo Della Coletta, Marcio Ajudarte Lopes, Oral Diagnosis Department, Piracicaba Dental School, University of Campinas (UNICAMP), Piracicaba, São Paulo 13414-903, Brazil.
authors.forename: Adriana Franco Paes
authors.initials: AF
authors.lastname: Leme
authors.affiliation: Isadora Luana Flores, Alan Roger Santos-Silva, Ricardo Della Coletta, Marcio Ajudarte Lopes, Oral Diagnosis Department, Piracicaba Dental School, University of Campinas (UNICAMP), Piracicaba, São Paulo 13414-903, Brazil.
authors.forename: Marcio Ajudarte
authors.initials: MA
authors.lastname: Lopes
keywords: Angiotensinogen; Biomarkers; Dipeptidyl peptidase 1; LC-MS/MS; Proliferative verrucous leukoplakia; Saliva