title: Production of Cellulases byRhizopus stoloniferfrom Glucose-Containing Media Based on the Regulation of Transcriptional Regulator CRE.
abstract: Carbon catabolite repression is a crucial regulation mechanism in microorganisms, but its characteristic inRhizopusis still unclear. We extracted a carbon regulation gene,cre, that encoded a carbon catabolite repressor protein (CRE) fromRhizopus stoloniferTP-02, and studied the regulation of CRE by real-time qPCR. CRE responded to glucose in a certain range, where it could significantly regulate part of the cellulase genes (eg, bg,andcbh2) withoutcbh1. In the comparison of the response ofcreand four cellulase genes to carboxymethylcellulose sodium and a simple carbon source (lactose), the effect of CRE was only related to the concentration of reducing sugars. By regulating the reducing sugars to range from 0.4% to 0.6%, a glucose-containing medium with lactose as the inducer could effectively induce cellulases without the repression of CRE. This regulation method could potentially reduce the cost of enzymes produced in industries and provide a possible solution to achieve the large-scale synthesis of cellulases.
authors.affiliation: School of Biotechnology, Jiangnan University, Wuxi 214000, P.R. China.
authors.forename: Yingyiing
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: School of Biotechnology, Jiangnan University, Wuxi 214000, P.R. China.|College of Biochemical Engineering, Anhui Polytechnic University, Wuhu 241000, P.R. China.
authors.forename: Bin
authors.initials: B
authors.lastname: Tang
authors.affiliation: School of Biotechnology, Jiangnan University, Wuxi 214000, P.R. China.
authors.forename: Guocheng
authors.initials: G
authors.lastname: Du
mesh_terms: D000595:Amino Acid Sequence; D044602:Cellulases / Q000096:biosynthesis* / Q000235:genetics; D003470:Culture Media; D015966:Gene Expression Regulation, Fungal; D005947:Glucose / Q000378:metabolism*; D012097:Repressor Proteins / Q000378:metabolism; D012233:Rhizopus / Q000235:genetics / Q000378:metabolism*; D013172:Spores, Fungal / Q000648:ultrastructure; D013379:Substrate Specificity; D014157:Transcription Factors / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D014158:Transcription, Genetic
chemical_list: D003470:Culture Media; D012097:Repressor Proteins; D014157:Transcription Factors; D044602:Cellulases; D005947:Glucose
keywords: Cellulases; Rhizopus stolonifer; carbon catabolite repressor; transcriptional regulation