title: Gomisin N Decreases Inflammatory Cytokine Production in Human Periodontal Ligament Cells.
abstract: Gomisin N, which is a lignan isolated from Schisandra chinensis, has some pharmacological effects. However, the anti-inflammatory effects of gomisin N on periodontal disease are uncertain. The aim of this study was to examine the effect of gomisin N on inflammatory mediator production in tumor necrosis factor (TNF)-α-stimulated human periodontal ligament cells (HPDLC). Gomisin N inhibited interleukin (IL)-6, IL-8, CC chemokine ligand (CCL) 2, and CCL20 production in TNF-α-stimulated HPDLC in a dose-dependent manner. Moreover, we revealed that gomisin N could suppress extracellular signal-regulated kinase (ERK) and c-Jun N terminal kinase (JNK) phosphorylation in TNF-α-stimulated HPDLC though protein kinase B (Akt) phosphorylation was not suppressed by gomisin N treatment. In summary, gomisin N might exert anti-inflammatory effects by attenuating cytokine production in periodontal ligament cells via inhibiting the TNF-α-stimulated ERK and JNK pathways activation.
authors.affiliation: Department of Conservative Dentistry, Institute of Biomedical Sciences, Tokushima University Graduate School, 3-18-15 Kuramoto-cho, Tokushima, Tokushima, 770-8504, Japan. hosokawa@tokushima-u.ac.jp.
authors.forename: Yoshitaka
authors.initials: Y
authors.lastname: Hosokawa
authors.affiliation: Department of Conservative Dentistry, Institute of Biomedical Sciences, Tokushima University Graduate School, 3-18-15 Kuramoto-cho, Tokushima, Tokushima, 770-8504, Japan.
authors.forename: Ikuko
authors.initials: I
authors.lastname: Hosokawa
authors.affiliation: Department of Conservative Dentistry, Institute of Biomedical Sciences, Tokushima University Graduate School, 3-18-15 Kuramoto-cho, Tokushima, Tokushima, 770-8504, Japan.
authors.forename: Satoru
authors.initials: S
authors.lastname: Shindo
authors.affiliation: Department of Oral Health Care Promotion, Institute of Biomedical Sciences, Tokushima University Graduate School, Tokushima, Tokushima, Japan.
authors.forename: Kazumi
authors.initials: K
authors.lastname: Ozaki
authors.affiliation: Department of Conservative Dentistry, Institute of Biomedical Sciences, Tokushima University Graduate School, 3-18-15 Kuramoto-cho, Tokushima, Tokushima, 770-8504, Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Matsuo
mesh_terms: D000893:Anti-Inflammatory Agents / Q000494:pharmacology; D002478:Cells, Cultured; D034242:Cyclooctanes / Q000494:pharmacology; D016207:Cytokines / Q000037:antagonists & inhibitors / Q000096:biosynthesis*; D004305:Dose-Response Relationship, Drug; D006801:Humans; D018836:Inflammation Mediators; D017705:Lignans / Q000494:pharmacology*; D020935:MAP Kinase Signaling System / Q000187:drug effects; D010513:Periodontal Ligament / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D010766:Phosphorylation / Q000187:drug effects; D011083:Polycyclic Compounds / Q000494:pharmacology*; D014409:Tumor Necrosis Factor-alpha
chemical_list: D000893:Anti-Inflammatory Agents; D034242:Cyclooctanes; D016207:Cytokines; D018836:Inflammation Mediators; D017705:Lignans; D011083:Polycyclic Compounds; D014409:Tumor Necrosis Factor-alpha; C015499:schizandrin B
keywords: MAPKs; gomisin N; inflammatory cytokine; periodontal ligament cells