title: Cerium chloride stimulated controlled conversion of B-to-Z DNA in self-assembled nanostructures.
abstract: DNA adopts different conformation not only because of novel base pairs but also while interacting with inorganic or organic compounds. Self-assembled branched DNA (bDNA) structures or DNA origami that change conformation in response to environmental cues hold great promises in sensing and actuation at the nanoscale. Recently, the B-Z transition in DNA is being explored to design various nanomechanical devices. In this communication we have demonstrated that Cerium chloride binds to the phosphate backbone of self-assembled bDNA structure and induce B-to-Z transition at physiological concentration. The mechanism of controlled conversion from right-handed to left-handed has been assayed by various dye binding studies using CD and fluorescence spectroscopy. Three different bDNA structures have been identified to display B-Z transition. This approach provides a rapid and reversible means to change bDNA conformation, which can be used for dynamic and progressive control at the nanoscale.
authors.affiliation: DNA Nanotechnology & Application Laboratory, CSIR-Institute of Minerals & Materials Technology, Bhubaneswar 751 013, India; Academy of Scientific & Innovative Research (AcSIR), New Delhi 110025, India.
authors.forename: Madhabi M
authors.initials: MM
authors.lastname: Bhanjadeo
authors.affiliation: DNA Nanotechnology & Application Laboratory, CSIR-Institute of Minerals & Materials Technology, Bhubaneswar 751 013, India.
authors.forename: Ashok K
authors.initials: AK
authors.lastname: Nayak
authors.affiliation: DNA Nanotechnology & Application Laboratory, CSIR-Institute of Minerals & Materials Technology, Bhubaneswar 751 013, India; Academy of Scientific & Innovative Research (AcSIR), New Delhi 110025, India. Electronic address: usubudhi@immt.res.in.
authors.forename: Umakanta
authors.initials: U
authors.lastname: Subudhi
mesh_terms: D001665:Binding Sites; D002563:Cerium / Q000737:chemistry*; D002942:Circular Dichroism; D059371:DNA, B-Form / Q000737:chemistry*; D043542:DNA, Z-Form / Q000737:chemistry*; D049329:Nanostructures / Q000737:chemistry*; D036103:Nanotechnology / Q000379:methods; D009690:Nucleic Acid Conformation; D009691:Nucleic Acid Denaturation; D013050:Spectrometry, Fluorescence
chemical_list: D059371:DNA, B-Form; D043542:DNA, Z-Form; D002563:Cerium; C026690:cerous chloride
keywords: B-Z transition; Cerium chloride; Conformation change; DNA Nanotechnology; Self-assembly; Z-DNA