title: Evaluating the Existence of Small Compressed Binucleated Squamous Cells in ASC-H
abstract: Purpose: To evaluate the legitimacy of a diagnosis of ASC-H in 5 cases which were followed up monthly for over 2
  years with both cytology and HPV testing. Methods: Some 5 cases out of a total of 25.0 self-sampled Pap test patients
  diagnosed as ASC-H provided 119 specimens over 2 years, with HPV-DNA testing perormed using a E6 primer. Results:
  Cases 1, 2 and 3 showed SIL after the ASC-H diagnosis, while cases 4 and 5 showed and maintained NILM. Cases 1,
  2 and 3 were further characterized by small atypical compressed binucleated cells, in which HPV was detected by in
  situ PCR. Case 4 showed a high N/C ratio in cells in sheets with a mild increase in chromatin. Case 5 demonstrated
  a high N/C ratio in small cells with no increase in chromatin. Conclusion: The finding of a compressed binucleated
  cells can define the difference between degenerated endocervical columnar cells and small atypical cells suggestive
  of HSIL. When small compres
authors.affiliation: Department of Medical Technology, Kyorin University, Faculty of Health Sciences, 5-4-1 Shimorenjaku Mitaka,181-0013,Tokyo, Japan. Email:ohkoudom@ks.kyorin-u.ac.jp
authors.forename: Mitsuaki
authors.initials: M
authors.lastname: Okodo
authors.forename: Kaori
authors.initials: K
authors.lastname: Okayama
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Kitamura
authors.forename: Natsuko
authors.initials: N
authors.lastname: Shiina
authors.forename: Timothy
authors.initials: T
authors.lastname: Caniz
authors.forename: Midori
authors.initials: M
authors.lastname: Ono
authors.forename: Hiromi
authors.initials: H
authors.lastname: Yabusaki
keywords: HPV; ASC-H; compressed binucleated squamous cells