title: High efficiency succinic acid production from glycerol via in situ fibrous bed bioreactor with an engineered Yarrowia lipolytica.
abstract: In this study, in situ fibrous bed bioreactor (isFBB) was developed at the first time for efficient succinic acid (SA) production by Yarrowia lipolytica. After optimization, SA titer, productivity and yield of 51.9g/L, 1.46g/L/h and 0.42g/g were obtained respectively via isFBB fermentation under conditions of 750cm2cotton towel, 120g/L initial glycerol and 3L/min aeration rate. By fed batch strategy, SA titer raised up to 198.2g/L was achieved, which was the highest value ever reported. In operation stability study, SA productivity showed no obvious decrease after 12 repeated batches of 460h fermentation, and cell viability even recovered within two repeated batches after intentional interruption. This study successfully attained a highly efficient and stable isFBB for enhanced SA production by Y. lipolytica.
authors.affiliation: School of Energy and Environment, City University of Hong Kong, Hong Kong.
authors.forename: Chong
authors.initials: C
authors.lastname: Li
authors.affiliation: Guangdong Provincial Key Laboratory of Fermentation and Enzyme Engineering, School of Bioscience and Bioengineering, South China University of Technology, Guangzhou 510006, People's Republic of China.
authors.forename: Xiaofeng
authors.initials: X
authors.lastname: Yang
authors.affiliation: School of Energy and Environment, City University of Hong Kong, Hong Kong.
authors.forename: Shi
authors.initials: S
authors.lastname: Gao
authors.affiliation: School of Energy and Environment, City University of Hong Kong, Hong Kong.
authors.forename: Huaimin
authors.initials: H
authors.lastname: Wang
authors.affiliation: School of Energy and Environment, City University of Hong Kong, Hong Kong. Electronic address: carollin@cityu.edu.hk.
authors.forename: Carol Sze Ki
authors.initials: CSK
authors.lastname: Lin
mesh_terms: D000332:Aerobiosis; D061249:Batch Cell Culture Techniques; D019149:Bioreactors / Q000382:microbiology*; D018914:Cells, Immobilized / Q000378:metabolism; D005240:Feasibility Studies; D005285:Fermentation; D005818:Genetic Engineering / Q000379:methods*; D005990:Glycerol / Q000378:metabolism*; D007700:Kinetics; D019802:Succinic Acid / Q000378:metabolism*; D025062:Yarrowia / Q000378:metabolism* / Q000648:ultrastructure
chemical_list: D019802:Succinic Acid; D005990:Glycerol
keywords: Fibrous bed bioreactor; Glycerol; Succinic acid; Yarrowia lipolytica