title: Screening of XylanolyticAspergillus fumigatusfor Prebiotic Xylooligosaccharide Production Using Bagasse.
abstract: Sugarcane bagasse is an important lignocellulosic material studied for the production of xylooligosaccharides (XOS). Some XOS are considered soluble dietary fibre, with low caloric value and prebiotic effect, but they are expensive and not easily available. In a screening of 138 fungi, only nine were shortlisted, and justAspergillus fumigatusM51 (35.6 U/mL) andA. fumigatusU2370 (28.5 U/mL) were selected as the most significant producers of xylanases. These fungi had low β-xylosidase activity, which is desirable for the production of XOS. The xylanases fromTrichoderma reeseiCCT 2768,A. fumigatusM51 andA. fumigatusU2370 gave a significantly higher XOS yield, 11.9, 14.7 and 7.9% respectively, in a 3-hour reaction with hemicellulose from sugarcane bagasse. These enzymes are relatively thermostable at 40-50 °C and can be used in a wide range of pH values. Furthermore, these xylanases produced more prebiotic XOS (xylobiose and xylotriose) when compared with a commercial xylanase. The xylanases fromA. fumigatusM51 reached a high level of XOS production (37.6%) in 48-72 h using hemicellulose extracted from sugarcane bagasse. This yield represents 68.8 kg of prebiotic XOS per metric tonne of cane bagasse. In addition, in a biorefinery, after hemicellulose extraction for XOS production, the residual cellulose could be used for the production of second-generation ethanol.
authors.affiliation: Department of Food Science, School of Food Engineering, State University of Campinas (UNICAMP), Rua Monteiro Lobato 80, 13083-862 Campinas, SP, Brazil; Department of Biological Sciences, School of Science and Letters, Săo Paulo State University (UNESP), Rua Dom Antonio 2100, 19806-380 Assis, SP, Brazil.
authors.forename: Ana Flavia Azevedo
authors.initials: AF
authors.lastname: Carvalho
authors.affiliation: Department of Biological Sciences, School of Science and Letters, Săo Paulo State University (UNESP), Rua Dom Antonio 2100, 19806-380 Assis, SP, Brazil.
authors.forename: Pedro de Oliva
authors.initials: PO
authors.lastname: Neto
authors.affiliation: Department of Biological Sciences, School of Science and Letters, Săo Paulo State University (UNESP), Rua Dom Antonio 2100, 19806-380 Assis, SP, Brazil.
authors.forename: Paula
authors.initials: P
authors.lastname: Zaghetto de Almeida
authors.affiliation: Department of Food Science, School of Food Engineering, State University of Campinas (UNICAMP), Rua Monteiro Lobato 80, 13083-862 Campinas, SP, Brazil.
authors.forename: Juliana
authors.initials: J
authors.lastname: Bueno da Silva
authors.affiliation: Department of Biological Sciences, School of Science and Letters, Săo Paulo State University (UNESP), Rua Dom Antonio 2100, 19806-380 Assis, SP, Brazil.
authors.forename: Bruna
authors.initials: B
authors.lastname: Escaramboni
authors.affiliation: Department of Food Science, School of Food Engineering, State University of Campinas (UNICAMP), Rua Monteiro Lobato 80, 13083-862 Campinas, SP, Brazil.
authors.forename: Glaucia Maria
authors.initials: GM
authors.lastname: Pastore
keywords: bagasse; lignocellulosic materials; xylanases; xylooligosaccharides