title: The Role of IL-33 on LPS-Induced Acute Lung Injury in Mice.
abstract: The objective of the study is to investigate the role and specific molecular mechanism of interleukin-33 (IL-33) acted on acute lung injury (ALI) induced by lipopolysaccharide (LPS). C57BL/6 mice intratracheally instilled LPS to induce ALI model. The mice were randomly divided into three groups: the sham operation group (Sham), ALI group (ALI), and pretreatment with IL-33 of ALI group (IL-33). By observing the survival rate, inflammatory cytokines in bronchoalveolar lavage fluid (BALF), myeloperoxidase (MPO) levels in lung tissue, lung histopathological examination, pulmonary capillary leakage, lung wet/dry (W/D) weight ratio, fibrosis levels in lung tissue, and associated pathways changes among the different groups, comparing to explore the role of IL-33 pretreatment on ALI mice and the possible molecular mechanisms. IL-33 pretreatment overall decreased the survival rate of ALI mice. IL-33 aggravated inflammation reaction showing as increasing the release of proinflammatory cytokines TNF-α and IL-6, increasing MPO levels in lung tissue, and aggravating lung pathology injury. In addition, IL-33 pretreatment further destroyed adherens junctions (AJs) by increasing the phosphorylation of VE-cadherin, resulting in the concomitantly pulmonary capillary barrier damage and pulmonary edema. During this process, mitogen-activated protein kinase (MAPK) pathways further activated. However, IL-33 pretreatment had no significant impact on collagen content of lung tissue. Our results indicated that IL-33 aggravated inflammatory reaction and increased microvascular permeability, but had little effect on pulmonary fibrosis, associated with the further activation of MAPK family proteins in the process. To sum up, IL-33 decreased survival rate and aggravated LPS-induced ALI.
authors.affiliation: Department of Anesthesiology, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, 3 Eastern Qingchun Road, Hangzhou, 310016, People's Republic of China.
authors.forename: Yaping
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Anesthesiology, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, 3 Eastern Qingchun Road, Hangzhou, 310016, People's Republic of China.
authors.forename: Ran
authors.initials: R
authors.lastname: Lv
authors.affiliation: Department of Anesthesiology, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, 3 Eastern Qingchun Road, Hangzhou, 310016, People's Republic of China.
authors.forename: Xuming
authors.initials: X
authors.lastname: Hu
authors.affiliation: Department of Anesthesiology, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, 3 Eastern Qingchun Road, Hangzhou, 310016, People's Republic of China.
authors.forename: Li
authors.initials: L
authors.lastname: Jiang
authors.affiliation: Department of Anesthesiology, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, 3 Eastern Qingchun Road, Hangzhou, 310016, People's Republic of China.
authors.forename: Dongju
authors.initials: D
authors.lastname: Xiao
authors.affiliation: Department of Anesthesiology, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, 3 Eastern Qingchun Road, Hangzhou, 310016, People's Republic of China.
authors.forename: Yv
authors.initials: Y
authors.lastname: Sun
authors.affiliation: Department of Anesthesiology, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, 3 Eastern Qingchun Road, Hangzhou, 310016, People's Republic of China.
authors.forename: Jinning
authors.initials: J
authors.lastname: Zhao
authors.affiliation: Department of Anesthesiology, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, 3 Eastern Qingchun Road, Hangzhou, 310016, People's Republic of China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Bao
authors.affiliation: Department of Anesthesiology, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, 3 Eastern Qingchun Road, Hangzhou, 310016, People's Republic of China. junranxie@163.com.
authors.forename: Junran
authors.initials: J
authors.lastname: Xie
mesh_terms: D055371:Acute Lung Injury / Q000139:chemically induced / Q000188:drug therapy*; D000818:Animals; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry; D002199:Capillary Permeability / Q000187:drug effects; D016207:Cytokines / Q000187:drug effects; D007249:Inflammation / Q000139:chemically induced; D000067596:Interleukin-33 / Q000008:administration & dosage / Q000494:pharmacology*; D008070:Lipopolysaccharides; D008168:Lung / Q000187:drug effects / Q000473:pathology; D051379:Mice; D008810:Mice, Inbred C57BL; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism; D015996:Survival Rate
chemical_list: D016207:Cytokines; D000067596:Interleukin-33; D008070:Lipopolysaccharides; D020928:Mitogen-Activated Protein Kinases
keywords: ALI; IL-33; MAPK