title: A human VE-cadherin-tdTomato and CD43-green fluorescent protein dual reporter cell line for study endothelial to hematopoietic transition.
abstract: Human embryonic stem cell line WA01 was genetically modified using zinc-finger nucleases and the PiggyBac/transponson system to introduce a fluorescence reporter for VE-cadherin (VEC; tdTomato) and CD43 (eGFP). Phenotypic and functional assays for pluripotency revealed the modified hES cell reporter lines remained normal. When the cells were differentiated into hematoendothelial lineages, either by directed differentiation or direct reprogramming, flow cytometric and fluorescence microscopy showed that VEC+ endothelial cells express tdTomato and CD43+ hematopoietic progenitors express eGFP.
authors.affiliation: Wisconsin National Primate Research Center, University of Wisconsin, Madison, WI 53715, USA.
authors.forename: Ho Sun
authors.initials: HS
authors.lastname: Jung
authors.affiliation: Wisconsin National Primate Research Center, University of Wisconsin, Madison, WI 53715, USA; Department of Pathology and Laboratory Medicine, University of Wisconsin, Madison, WI 53792, USA.
authors.forename: Gene
authors.initials: G
authors.lastname: Uenishi
authors.affiliation: Wisconsin National Primate Research Center, University of Wisconsin, Madison, WI 53715, USA.
authors.forename: Akhilesh
authors.initials: A
authors.lastname: Kumar
authors.affiliation: Wisconsin National Primate Research Center, University of Wisconsin, Madison, WI 53715, USA.
authors.forename: Mi Ae
authors.initials: MA
authors.lastname: Park
authors.affiliation: Wisconsin National Primate Research Center, University of Wisconsin, Madison, WI 53715, USA.
authors.forename: Matt
authors.initials: M
authors.lastname: Raymond
authors.affiliation: Wisconsin National Primate Research Center, University of Wisconsin, Madison, WI 53715, USA.
authors.forename: Dustin
authors.initials: D
authors.lastname: Fink
authors.affiliation: Wisconsin National Primate Research Center, University of Wisconsin, Madison, WI 53715, USA.
authors.forename: Ethan
authors.initials: E
authors.lastname: McLeod
authors.affiliation: Wisconsin National Primate Research Center, University of Wisconsin, Madison, WI 53715, USA; Department of Pathology and Laboratory Medicine, University of Wisconsin, Madison, WI 53792, USA. Electronic address: islukvin@wisc.edu.
authors.forename: Igor
authors.initials: I
authors.lastname: Slukvin
mesh_terms: D015703:Antigens, CD / Q000235:genetics / Q000378:metabolism*; D015820:Cadherins / Q000235:genetics / Q000378:metabolism*; D002454:Cell Differentiation; D002478:Cells, Cultured; D058732:Embryoid Bodies / Q000378:metabolism / Q000473:pathology; D042783:Endothelial Cells / Q000166:cytology / Q000378:metabolism; D017930:Genes, Reporter; D005822:Genetic Vectors / Q000235:genetics / Q000378:metabolism; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism*; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000378:metabolism; D000066449:Human Embryonic Stem Cells / Q000166:cytology / Q000378:metabolism; D006801:Humans; D059785:Karyotype; D051917:Leukosialin / Q000235:genetics / Q000378:metabolism*; D008297:Male; D008856:Microscopy, Fluorescence; D059008:Time-Lapse Imaging; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D000099152:Cadherin 5
chemical_list: D015703:Antigens, CD; D015820:Cadherins; D049452:Green Fluorescent Proteins; D051917:Leukosialin; D014157:Transcription Factors; D000099152:Cadherin 5