title: Spirosoma swuense sp. nov., isolated from wet soil.
abstract: Strain JBM2-3T, a pale-yellow-coloured, aerobic, catalase-negative, oxidase-positive and Gram-stain-negative bacterium, was isolated from wet soil. The isolate grew aerobically at 25-30 °C (optimum 25 °C), pH 6.0-8.0 (optimum pH 7.0) and in the presence of 0-0.5 % (w/v) NaCl (optimum 0 % NaCl). Phylogenetic analysis based on 16S rRNA gene sequence indicated that strain JBM2-3T belonged to the genus Spirosoma, with a sequence similarity of 96.2 % with Spirosoma panaciterrae Gsoil 1519T. The strain showed the typical chemotaxonomic characteristics of the genus Spirosoma, with the presence of menaquinone 7 as the respiratory quinone; the major fatty acids were summed feature 3 (composed of C16: 1ω6c/ω7c), C16: 1ω5c and iso-C15: 0. The DNA G+C content of strain JBM2-3T was 47.4 mol%. The polar lipid profile contained major amounts of phosphatidylethanolamine and aminophospholipids. On the basis of its phenotypic and genotypic properties, and phylogenetic distinctiveness, strain JBM2-3T should be classified as a representative of a novel species in the genus Spirosoma, for which the name Spirosoma swuense sp. nov. is proposed. The type strain is JBM2-3T (=KCTC 52176T=JCM 31298T).
authors.affiliation: Department of Bio and Environmental Technology, College of Natural Science, Seoul Women's University, Seoul 139-774, Republic of Korea.
authors.forename: Eun Sun
authors.initials: ES
authors.lastname: Joo
authors.affiliation: Department of Bio and Environmental Technology, College of Natural Science, Seoul Women's University, Seoul 139-774, Republic of Korea.
authors.forename: Eun Bit
authors.initials: EB
authors.lastname: Kim
authors.affiliation: Department of Bio and Environmental Technology, College of Natural Science, Seoul Women's University, Seoul 139-774, Republic of Korea.
authors.forename: Seon Hwa
authors.initials: SH
authors.lastname: Jeon
authors.affiliation: Department of Bio and Environmental Technology, College of Natural Science, Seoul Women's University, Seoul 139-774, Republic of Korea.
authors.forename: Sathiyaraj
authors.initials: S
authors.lastname: Srinivasan
authors.affiliation: Department of Bio and Environmental Technology, College of Natural Science, Seoul Women's University, Seoul 139-774, Republic of Korea.
authors.forename: Myung Kyum
authors.initials: MK
authors.lastname: Kim
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D041964:Cytophagaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D010743:Phospholipids / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D056910:Republic of Korea; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S