title: Butyrate pretreatment attenuates heart depression in a mice model of endotoxin-induced sepsis via anti-inflammation and anti-oxidation.
abstract: OBJECTIVE
  The depressed heart function is the main complication to cause death of septic patients in clinic. It is urgent to find effective interventions for this intractable disease. In this study, we investigated whether butyrate could be protective for heart against sepsis and the underlying mechanism.
  
  
  METHODS
  Mice were randomly divided into three groups. Model group challenged with LPS (30 mg/kg, i.p.) only. Butyrate group received butyrate (200 mg/kg·d) for 3days prior to LPS administration (30 mg/kg). Normal group received saline only. 6h and 12h after LPS administration were chosen for detection the parameters to estimate the effects or mechanism of butyrate pretreatment on heart of sepsis.
  
  
  RESULTS
  The data showed that septic heart depression was attenuated by butyrate pretreatment through improvement of heart function depression (P<0.01) and reduction of morphological changes of myocardium. The overexpression of proinflammatory factors, TNF-α, IL-6 and LTB4, in heart tissues induced by sepsis was significantly alleviated by butyrate pretreatment (P<0.01). As oxidative stress indicators, SOD and CAT activity, and MDA content in heart were deteriorated by LPS challenge, which was noticeably ameliorated by butyrate pretreatment (P<0.01 or P<0.05).
  
  
  CONCLUSIONS
  In conclusion, pretreatment with butyrate attenuated septic heart depression via anti-inflammation and anti-oxidation.
authors.affiliation: Department of Pathophysiology, Wenzhou Medical University, Zhejiang Province, China; Children's Heart Center, The Second Affiliated Hospital & Yuying Children's Hospital, Institute of Cardiovascular Development and Translational Medicine, Wenzhou Medical University, Zhejiang Province, China.
authors.forename: Fangyan
authors.initials: F
authors.lastname: Wang
authors.affiliation: Department of Pediatrics of The First Affiliated Hospital, Wenzhou Medical University, Zhejiang Province, China.
authors.forename: Zengyou
authors.initials: Z
authors.lastname: Jin
authors.affiliation: Department of Pathophysiology, Wenzhou Medical University, Zhejiang Province, China.
authors.forename: Kaiyi
authors.initials: K
authors.lastname: Shen
authors.affiliation: Department of Pathophysiology, Wenzhou Medical University, Zhejiang Province, China.
authors.forename: Tingting
authors.initials: T
authors.lastname: Weng
authors.affiliation: Department of Pathophysiology, Wenzhou Medical University, Zhejiang Province, China.
authors.forename: Zhisong
authors.initials: Z
authors.lastname: Chen
authors.affiliation: Department of Pathophysiology, Wenzhou Medical University, Zhejiang Province, China.
authors.forename: Jiahui
authors.initials: J
authors.lastname: Feng
authors.affiliation: Department of Pathophysiology, Wenzhou Medical University, Zhejiang Province, China.
authors.forename: Zhengzheng
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: School of Environmental Science and Public Health, Wenzhou Medical University, Zhejiang Province, China. Electronic address: wzljm@wzmc.edu.cn.
authors.forename: Jiaming
authors.initials: J
authors.lastname: Liu
authors.affiliation: Department of Intensive Care Unit of The Second Affiliated Hospital, Wenzhou Medical University, Zhejiang Province, China.
authors.forename: Xiaolong
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Children's Heart Center, The Second Affiliated Hospital & Yuying Children's Hospital, Institute of Cardiovascular Development and Translational Medicine, Wenzhou Medical University, Zhejiang Province, China. Electronic address: chmping@hotmail.com.
authors.forename: Maoping
authors.initials: M
authors.lastname: Chu
mesh_terms: D000818:Animals; D002087:Butyrates / Q000008:administration & dosage*; D018890:Chemoprevention / Q000379:methods; D004195:Disease Models, Animal; D006335:Heart Injuries / Q000209:etiology / Q000517:prevention & control*; D008070:Lipopolysaccharides; D008297:Male; D051379:Mice; D018384:Oxidative Stress / Q000187:drug effects*; D018805:Sepsis / Q000150:complications* / Q000188:drug therapy
chemical_list: D002087:Butyrates; D008070:Lipopolysaccharides
keywords: Butyrate; Cytokines; Heart depression; Inflammation; Oxidative stress; Sepsis