title: Sialyltransferase ST3GAL6 mediates the effect of microRNA-26a on cell growth, migration, and invasion in hepatocellular carcinoma through the protein kinase B/mammalian target of rapamycin pathway.
abstract: Aberrant sialylation profiles on the cell surface have been recognized for their potential diagnostic value in identifying the regulation of tumor properties in several cancers, including hepatocellular carcinoma (HCC). Recently, increasing evidence has suggested that the deregulation of microRNA (miRNA) is a common feature in human cancers. In this study, we found obvious upregulation of sialyltransferase ST3GAL6 both in HCC cell lines and in tissue samples. The altered expression of ST3GAL6 was found to correlate with cell proliferation, migration, and invasion ability in HCC. Further investigation showed that miR-26a negatively regulated ST3GAL6, inducing the suppression of cell proliferation, migration, and invasion in vitro. Moreover, we identified the protein kinase B/mammalian target of rapamycin (Akt/mTOR) pathway as the target of ST3GAL6 based on Western blot analysis. Analysis of a xenograft mouse model showed that miR-26a significantly reduced tumor growth by suppressing activation of the Akt/mTOR pathway by directly targeting ST3GAL6. In conclusion, these data indicate that ST3GAL6 promotes cell growth, migration, and invasion and mediates the effect of miR-26a through the Akt/mTOR signaling pathway in HCC.
authors.affiliation: Department of General Surgery, The Second Affiliated Hospital of Dalian Medical University, Dalian, China.
authors.forename: Mingming
authors.initials: M
authors.lastname: Sun
authors.affiliation: School of Basic Medical Sciences, Hebei Medical University, Shijiazhuang, China.
authors.forename: Xuzi
authors.initials: X
authors.lastname: Zhao
authors.affiliation: Department of General Surgery, The Second Affiliated Hospital of Dalian Medical University, Dalian, China.
authors.forename: Leilei
authors.initials: L
authors.lastname: Liang
authors.affiliation: Department of General Surgery, The Second Affiliated Hospital of Dalian Medical University, Dalian, China.
authors.forename: Xufeng
authors.initials: X
authors.lastname: Pan
authors.affiliation: Department of Orthopedics, The Second Affiliated Hospital of Dalian Medical University, Dalian, China.
authors.forename: Hao
authors.initials: H
authors.lastname: Lv
authors.affiliation: Department of General Surgery, The Second Affiliated Hospital of Dalian Medical University, Dalian, China.
authors.forename: Yongfu
authors.initials: Y
authors.lastname: Zhao
mesh_terms: D000818:Animals; D006528:Carcinoma, Hepatocellular / Q000378:metabolism / Q000473:pathology* / Q000556:secondary; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D006801:Humans; D008113:Liver Neoplasms / Q000378:metabolism / Q000473:pathology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D035683:MicroRNAs / Q000502:physiology*; D009361:Neoplasm Invasiveness; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism*; D012799:Sialyltransferases / Q000502:physiology*; D015398:Signal Transduction; D058570:TOR Serine-Threonine Kinases / Q000378:metabolism*; D014410:Tumor Stem Cell Assay; D015854:Up-Regulation; D000094663:beta-Galactoside alpha-2,3-Sialyltransferase
chemical_list: D035683:MicroRNAs; D051057:Proto-Oncogene Proteins c-akt; D012799:Sialyltransferases; D058570:TOR Serine-Threonine Kinases; D000094663:beta-Galactoside alpha-2,3-Sialyltransferase; C528163:MIRN26 microRNA, human; C546842:MTOR protein, human
keywords: Akt/mTOR pathway; ST3GAL6; growth, invasion and migration; hepatocellular carcinoma; miR-26a