title: Effects of allitridum on the transient outward potassium current in rats with heart failure.
abstract: OBJECTIVE
  To study the effect of allitridum on the transient outward potassium current (Ito) of ventricular myocytes in heart failure (HF).
  
  
  METHODS
  The dual enzymatic method was used to separate single ventricular myocytes from Sprague Dawley rats. Patch-clamping was used to recordItoand analyze the effect of allitridum on the current.
  
  
  RESULTS
  TheItocurrent had a significant decrease in the HF group, compared with the control group. The density ofItoin the HF group was increased after treatment of allitridum (30 µmol/L). The peak current densities ofItowere enhanced in the HF group from 6.01 ± 0.30 pA/pF to 8.41 ± 0.54 pA/pF (P< 0.01) at +50 mV after treatment with allitridum (30 µmol/L). We also determined the effect of allitridum on the gating mechanism of theItoin the HF group.
  
  
  CONCLUSIONS
  We found that allitridum increased theItoby accelerating the activation of channels and shortened the time constants of inactivation, and allitridum decreased the remodeling ofItoin ventricular myocytes of rats with HF.
authors.affiliation: Department of Cardiology, Chinese PLA General Hospital, Beijing, China.
authors.forename: Xiao-Jing
authors.initials: XJ
authors.lastname: Zhao
authors.affiliation: Department of Cardiology, Chinese PLA General Hospital, Beijing, China.
authors.forename: Kun
authors.initials: K
authors.lastname: Lin
authors.affiliation: Department of Cardiology, Chinese PLA General Hospital, Beijing, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Cardiology, Chinese PLA General Hospital, Beijing, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Xu
authors.affiliation: Department of Cardiology, Chinese PLA General Hospital, Beijing, China.
authors.forename: Li
authors.initials: L
authors.lastname: Liu
authors.affiliation: Department of Cardiology, Chinese PLA General Hospital, Beijing, China.
authors.forename: Yi-Cheng
authors.initials: YC
authors.lastname: Fu
authors.affiliation: Department of Cardiology, Chinese PLA General Hospital, Beijing, China.
authors.forename: Xi
authors.initials: X
authors.lastname: Chen
authors.affiliation: Department of Cardiology, Chinese PLA General Hospital, Beijing, China.
authors.forename: Zhong-Qi
authors.initials: ZQ
authors.lastname: Cai
authors.affiliation: Department of Cardiology, Chinese PLA General Hospital, Beijing, China.
authors.forename: Zhi-Juan
authors.initials: ZJ
authors.lastname: Wu
authors.affiliation: Department of Gerontology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Huang
authors.affiliation: Department of Cardiology, Chinese PLA General Hospital, Beijing, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Li
keywords: Allitridum; Heart failure; Ion current; Ventricular myocyte