title: Comparative Genomics of DNA Recombination and Repair in Cyanobacteria: Biotechnological Implications.
abstract: Cyanobacteria are fascinating photosynthetic prokaryotes that are regarded as the ancestors of the plant chloroplast; the purveyors of oxygen and biomass for the food chain; and promising cell factories for an environmentally friendly production of chemicals. In colonizing most waters and soils of our planet, cyanobacteria are inevitably challenged by environmental stresses that generate DNA damages. Furthermore, many strains engineered for biotechnological purposes can use DNA recombination to stop synthesizing the biotechnological product. Hence, it is important to study DNA recombination and repair in cyanobacteria for both basic and applied research. This review reports what is known in a few widely studied model cyanobacteria and what can be inferred by mining the sequenced genomes of morphologically and physiologically diverse strains. We show that cyanobacteria possess manyE. coli-like DNA recombination and repair genes, and possibly other genes not yet identified.E. coli-homolog genes are unevenly distributed in cyanobacteria, in agreement with their wide genome diversity. Many genes are extremely well conserved in cyanobacteria (mutMS, radA, recA, recFO, recG, recN, ruvABC, ssb, anduvrABCD), even in small genomes, suggesting that they encode the core DNA repair process. In addition to these core genes, the marineProchlorococcusandSynechococcusstrains harborrecBCD(DNA recombination),umuCD(mutational DNA replication), as well as the key SOS geneslexA(regulation of the SOS system) andsulA(postponing of cell division until completion of DNA reparation). Hence, these strains could possess anE. coli-type SOS system. In contrast, several cyanobacteria endowed with larger genomes lack typical SOS genes. For examples, the two studiedGloeobacterstrains lackalkB, lexA, andsulA; andSynechococcusPCC7942 has neitherlexAnorrecCD. Furthermore, theSynechocystisPCC6803lexAproduct does not regulate DNA repair genes. Collectively, these findings indicate that not all cyanobacteria have anE. coli-type SOS system. Also interestingly, several cyanobacteria possess multiple copies ofE. coli-like DNA repair genes, such asAcaryochloris marinaMBIC11017 (2alkB, 3ogt, 7recA, 3recD, 2ssb, 3umuC, 4umuD, and 8xerC),CyanotheceATCC51142 (2lexAand 4ruvC), andNostocPCC7120 (2ssband 3xerC).
authors.affiliation: Institute for Integrative Biology of the Cell, CEA, Centre Nationnal de la Recherche Scientifique (CNRS), Universite Paris-Sud, Université Paris-Saclay Gif-sur-Yvette Cedex, France.
authors.forename: Corinne
authors.initials: C
authors.lastname: Cassier-Chauvat
authors.affiliation: Institute for Integrative Biology of the Cell, CEA, Centre Nationnal de la Recherche Scientifique (CNRS), Universite Paris-Sud, Université Paris-Saclay Gif-sur-Yvette Cedex, France.
authors.forename: Théo
authors.initials: T
authors.lastname: Veaudor
authors.affiliation: Institute for Integrative Biology of the Cell, CEA, Centre Nationnal de la Recherche Scientifique (CNRS), Universite Paris-Sud, Université Paris-Saclay Gif-sur-Yvette Cedex, France.
authors.forename: Franck
authors.initials: F
authors.lastname: Chauvat
keywords: DNA recombination; DNA repair; cyanobacteria; genetic instability; insertion sequences; natural transformation; photoproduction; radiation resistance