title: Isoflavone genistein inhibits estrogen-induced chloride and bicarbonate secretory mechanisms in the uterus in rats.
abstract: We hypothesized that genistein could affect the chloride (Cl-) and bicarbonate (HCO3-) secretory mechanisms in uterus. Ovariectomized female rats were given estradiol or estradiol plus progesterone with 25, 50, or 100 mg/kg/day genistein. Following completion of the treatment, uterine fluid Cl-and HCO3-concentrations were determined by in vivo uterine perfusion. Uteri were subjected for molecular biological analysis (Western blot, qPCR, and immunohistochemistry) to detect levels of expression of Cystic Fibrosis transmembrane regulator (CFTR), Cl-/HCO3-exchanger (SLC26a6), Na+/HCO3-cotransporter (SLC4a4), and estrogen receptor (ER)-α and β. Coadministration of genistein resulted in decrease in Cl-and HCO3-concentrations and expression of CFTR, SLC26a6, SLC4a4, and ER-α and ER-β in the uteri of estradiol-treated rats. In estradiol plus progesterone-treated rats, a significant increase in the above parameters were observed following high-dose genistein treatment except for the SLC24a4 level. In conclusion, genistein-induced changes in the uterus could affect the reproductive processes that might result in infertility.
authors.affiliation: Department of Physiology, Faculty of Medicine, University of Malaya, Lembah Pantai, Kuala Lumpur, 50603, Malaysia.
authors.forename: Asma
authors.initials: A
authors.lastname: Chinigarzadeh
authors.affiliation: Department of Physiology, Faculty of Medicine, University of Malaya, Lembah Pantai, Kuala Lumpur, 50603, Malaysia.
authors.forename: Kamarulzaman
authors.initials: K
authors.lastname: Karim
authors.affiliation: Department of Molecular Medicine, Faculty of Medicine, University of Malaya, Lembah Pantai, Kuala Lumpur, 50603, Malaysia.
authors.forename: Sekaran
authors.initials: S
authors.lastname: Muniandy
authors.affiliation: Department of Physiology, Faculty of Medicine, University of Malaya, Lembah Pantai, Kuala Lumpur, 50603, Malaysia.
authors.forename: Naguib
authors.initials: N
authors.lastname: Salleh
mesh_terms: D000818:Animals; D017920:Antiporters / Q000187:drug effects / Q000235:genetics; D001639:Bicarbonates / Q000378:metabolism*; D002712:Chlorides / Q000378:metabolism*; D019005:Cystic Fibrosis Transmembrane Conductance Regulator / Q000187:drug effects / Q000235:genetics; D004967:Estrogens / Q000494:pharmacology*; D005260:Female; D005786:Gene Expression Regulation; D019833:Genistein / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D011960:Receptors, Estrogen / Q000187:drug effects / Q000235:genetics; D027982:Sodium-Bicarbonate Symporters / Q000187:drug effects / Q000235:genetics; D000076988:Sulfate Transporters; D014599:Uterus / Q000187:drug effects* / Q000378:metabolism
chemical_list: D017920:Antiporters; D001639:Bicarbonates; C579654:CFTR protein, rat; D002712:Chlorides; D004967:Estrogens; D011960:Receptors, Estrogen; C579655:SLC26A6 protein, rat; C422004:Slc4a4 protein, rat; D027982:Sodium-Bicarbonate Symporters; D000076988:Sulfate Transporters; D019005:Cystic Fibrosis Transmembrane Conductance Regulator; D019833:Genistein
keywords: bicarbonate; genistein; sex-steroids; uterine fluid chloride