title: Pathogenesis of Senecavirus A infection in finishing pigs.
abstract: Senecavirus A (SVA) is an emerging picornavirus that has been associated with vesicular disease and neonatal mortality in swine. Many aspects of SVA infection biology and pathogenesis, however, remain unknown. Here the pathogenesis of SVA was investigated in finishing pigs. Animals were inoculated via the oronasal route with SVA strain SD15-26 and monitored for clinical signs and lesions associated with SVA infection. Viraemia was assessed in serum and virus shedding monitored in oral and nasal secretions and faeces by real-time reverse transcriptase quantitative PCR (RT-qPCR) and/or virus isolation. Additionally, viral load and tissue distribution were assessed during acute infection and following convalescence from disease. Clinical signs characterized by lethargy and lameness were first observed on day 4 post-inoculation (pi) and persisted for approximately 2-10 days. Vesicular lesions were first observed on day 4 pi on the snout and/or feet, affecting the coronary bands, dewclaws, interdigital space and heel/sole of SVA-infected animals. A short-term viraemia was observed between days 3 and 10 pi, whereas virus shedding was detected between days 1 and 28 pi in oral and nasal secretions and faeces. Notably, RT-qPCR and in situ hybridization (ISH) performed on tissues collected on day 38 pi revealed the presence of SVA RNA in the tonsils of all SVA-infected animals. Serological responses to SVA were characterized by early neutralizing antibody responses (day 5 pi), which coincided with decreased levels of viraemia, virus shedding and viral load in tissues. This study provides significant insights into the pathogenesis and infectious dynamics of SVA in swine.
authors.affiliation: Animal Disease Research and Diagnostic Laboratory, Department of Veterinary and Biomedical Sciences, South Dakota State University, Brookings, SD, USA.
authors.forename: Lok R
authors.initials: LR
authors.lastname: Joshi
authors.affiliation: Animal Disease Research and Diagnostic Laboratory, Department of Veterinary and Biomedical Sciences, South Dakota State University, Brookings, SD, USA.
authors.forename: Maureen H V
authors.initials: MHV
authors.lastname: Fernandes
authors.affiliation: Animal Disease Research and Diagnostic Laboratory, Department of Veterinary and Biomedical Sciences, South Dakota State University, Brookings, SD, USA.
authors.forename: Travis
authors.initials: T
authors.lastname: Clement
authors.affiliation: Animal Disease Research and Diagnostic Laboratory, Department of Veterinary and Biomedical Sciences, South Dakota State University, Brookings, SD, USA.
authors.forename: Steven
authors.initials: S
authors.lastname: Lawson
authors.affiliation: Animal Disease Research and Diagnostic Laboratory, Department of Veterinary and Biomedical Sciences, South Dakota State University, Brookings, SD, USA.
authors.forename: Angela
authors.initials: A
authors.lastname: Pillatzki
authors.affiliation: Veterinary Diagnostic Laboratory, University of Minnesota, St. Paul, MN, USA.
authors.forename: Talita P
authors.initials: TP
authors.lastname: Resende
authors.affiliation: Veterinary Diagnostic Laboratory, University of Minnesota, St. Paul, MN, USA.
authors.forename: Fabio A
authors.initials: FA
authors.lastname: Vannucci
authors.affiliation: Department of Pathobiology, University of Connecticut, Storrs, CT, USA.
authors.forename: Gerald F
authors.initials: GF
authors.lastname: Kutish
authors.affiliation: Animal Disease Research and Diagnostic Laboratory, Department of Veterinary and Biomedical Sciences, South Dakota State University, Brookings, SD, USA.
authors.forename: Eric A
authors.initials: EA
authors.lastname: Nelson
authors.affiliation: Animal Disease Research and Diagnostic Laboratory, Department of Veterinary and Biomedical Sciences, South Dakota State University, Brookings, SD, USA.
authors.forename: Diego G
authors.initials: DG
authors.lastname: Diel
mesh_terms: D000818:Animals; D057134:Antibodies, Neutralizing / Q000097:blood; D000914:Antibodies, Viral / Q000097:blood; D010849:Picornaviridae / Q000235:genetics / Q000302:isolation & purification / Q000472:pathogenicity* / Q000502:physiology; D013552:Swine; D013553:Swine Diseases / Q000097:blood / Q000473:pathology / Q000821:virology*; D019562:Viral Load; D014766:Viremia / Q000097:blood / Q000473:pathology / Q000662:veterinary* / Q000821:virology; D014774:Virulence; D017201:Virus Shedding
chemical_list: D057134:Antibodies, Neutralizing; D000914:Antibodies, Viral