title: RAPID COMMUNICATION: Isolation of glucose transporters and in bovine uteroplacental tissues from days 16 to 50 of gestation.
abstract: Glucose transporter solute carrier family 2 member 14 () is a duplicon of glucose transporter solute carrier family 2 member 3 () with a 95% shared homology to and has not previously been isolated in ruminant uteroplacental tissues. The transporter has been previously isolated in Holstein heifer uterine epithelium but not in ovine epithelium. We hypothesized that and its duplicon would be found in bovine uteroplacental tissues and that maternal nutrition and day of gestation would impact mRNA expression of and . Crossbred Angus heifers ( = 49) were estrus synchronized, bred via AI, and assigned to nutritional treatment (CON = 100% of requirements to gain 0.45 kg/d; RES = 60% of CON) at breeding. Ovariohysterectomy was performed on d 16, 34, or 50 of gestation ( = 6 to 9/d); nonpregnant (NP) controls were not bred and ovariohysterectomized on d 16 of the synchronized estrous cycle ( = 6). The resulting treatment arrangement was a 2 × 3 factorial + 1. Uteroplacental tissues (caruncle, CAR; intercaruncular endometrium, ICAR; and fetal membrane [chorioallantois], FM) were obtained from the pregnant uterine horn immediately after ovariohysterectomy. For NP controls, only CAR and ICAR were obtained. There were no day × treatment interactions for or gene expression in CAR, ICAR, or FM. Expression of in CAR was greater ( = 0.03) on d 50 compared with d 16. In ICAR, was greatest ( = 0.02) on d 50 compared with d 16 and 34 of gestation. In FM, was greater ( = 0.04) on d 16 compared with d 50. Expression of was greater ( = 0.05) in pregnant compared with nonpregnant heifers. Additionally, expression of was greater ( = 0.01) on d 34 and 50 compared with d 16. Expression of in CAR was greater ( = 0.03) on d 50 compared to d 16 and 34. In CAR, tended ( = 0.07) to be greater on d 34 and 50 than on d 16 and was greater ( = 0.02) on d 50 than on d 34. There was no effect of treatment for either or in CAR, ICAR, or FM. These data demonstrate that glucose transporters and are expressed in beef heifer uteroplacental tissues and that they are expressed differentially by day of gestation in bovine uteroplacental tissues.
authors.forename: M S
authors.initials: MS
authors.lastname: Crouse
authors.forename: J S
authors.initials: JS
authors.lastname: Caton
authors.forename: K J
authors.initials: KJ
authors.lastname: McLean
authors.forename: P P
authors.initials: PP
authors.lastname: Borowicz
authors.forename: L P
authors.initials: LP
authors.lastname: Reynolds
authors.forename: C R
authors.initials: CR
authors.lastname: Dahlen
authors.forename: B W
authors.initials: BW
authors.lastname: Neville
authors.forename: A K
authors.initials: AK
authors.lastname: Ward
mesh_terms: D000818:Animals; D001483:Base Sequence; D001947:Breeding; D002417:Cattle / Q000235:genetics* / Q000502:physiology; D004717:Endometrium / Q000502:physiology; D004973:Estrus Synchronization; D005260:Female; D015870:Gene Expression; D051246:Glucose Transport Proteins, Facilitative / Q000235:genetics* / Q000378:metabolism; D010920:Placenta / Q000502:physiology; D011247:Pregnancy; D011270:Pregnancy, Animal; D039401:Prenatal Nutritional Physiological Phenomena; D016415:Sequence Alignment / Q000662:veterinary; D014599:Uterus / Q000502:physiology
chemical_list: D051246:Glucose Transport Proteins, Facilitative