title: Systematic investigation on the turning point of over-inflammation to immunosuppression in CLP mice model and their characteristics.
abstract: Immunosuppression is involved in refractory innate and adaptive immune responses and is considered to be the predominant driving force for mortality in sepsis. The cecal ligation and puncture (CLP) model is regarded as a golden standard model for sepsis study, but the turning point of over-inflammation to immunosuppression was reported differently. Herein, systematic investigation on the turning point of over-inflammation to immunosuppression in CLP mice model was carried out. The results showed only the mortality of mice challenged with of Pseudomonas aeruginosa on Day 1 not other days after the surgery was higher than that of other mice with Sham surgery, suggesting Day 1 after the CLP surgery might be the turning point. There was very low mortality even without death in Sham mice but the mortality was 80% after mice were challenged with 2.5×107, 5.0×106and 1.0×106CFU/10g of Pseudomonas aeruginosa, further demonstrating Day 1 after the CLP surgery was the turning point. And, CLP mice presented low levels of pro-inflammatory and anti-inflammatory cytokines, and high bacterial loads on Day 1. Additionally, the amounts and proportion of blood cells and monocytes significantly changed, too. In conclusion, Day 1 after the CLP surgery was the turning point of over-inflammation to immunosuppression, and low levels of cytokines and high bacterial loads were the characteristics of this model on Day 1, which is significant for pharmacological investigation on sepsis.
authors.affiliation: Department of Pharmacology, College of Pharmacy, the Third Military Medical University, Chongqing 400038, PR China.
authors.forename: Dongmei
authors.initials: D
authors.lastname: Deng
authors.affiliation: Department of Pharmacology, College of Pharmacy, the Third Military Medical University, Chongqing 400038, PR China.
authors.forename: Xiaoli
authors.initials: X
authors.lastname: Li
authors.affiliation: Department of Pharmacology, College of Pharmacy, the Third Military Medical University, Chongqing 400038, PR China.
authors.forename: Chao
authors.initials: C
authors.lastname: Liu
authors.affiliation: Department of Pharmacology, College of Pharmacy, the Third Military Medical University, Chongqing 400038, PR China.
authors.forename: Zhaoxia
authors.initials: Z
authors.lastname: Zhai
authors.affiliation: Department of Pharmacology, College of Pharmacy, the Third Military Medical University, Chongqing 400038, PR China.
authors.forename: Bin
authors.initials: B
authors.lastname: Li
authors.affiliation: Department of Pharmacology, College of Pharmacy, the Third Military Medical University, Chongqing 400038, PR China.
authors.forename: Mei
authors.initials: M
authors.lastname: Kuang
authors.affiliation: Department of Pharmacology, College of Pharmacy, the Third Military Medical University, Chongqing 400038, PR China.
authors.forename: Pan
authors.initials: P
authors.lastname: Li
authors.affiliation: Department of Pharmacy, Southwest Hospital, the Third Military Medical University, Chongqing 400038, PR China.
authors.forename: Shenglan
authors.initials: S
authors.lastname: Shang
authors.affiliation: Department of Pharmacology, College of Pharmacy, the Third Military Medical University, Chongqing 400038, PR China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Song
authors.affiliation: Department of Pharmacology, College of Pharmacy, the Third Military Medical University, Chongqing 400038, PR China.
authors.forename: Yanyan
authors.initials: Y
authors.lastname: Cen
authors.affiliation: Department of Pharmacology, College of Pharmacy, the Third Military Medical University, Chongqing 400038, PR China.
authors.forename: Rongxin
authors.initials: R
authors.lastname: Qin
authors.affiliation: Medical Research Center, Southwest Hospital, the Third Military Medical University, Chongqing, 400038, P.R. China.
authors.forename: Yonglin
authors.initials: Y
authors.lastname: Lu
authors.affiliation: Company 18th, College of Pharmacy, the Third Military Medical University, Chongqing 400038, PR China.
authors.forename: Yibo
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: Company 18th, College of Pharmacy, the Third Military Medical University, Chongqing 400038, PR China.
authors.forename: Hao
authors.initials: H
authors.lastname: Cheng
authors.affiliation: Medical Research Center, Southwest Hospital, the Third Military Medical University, Chongqing, 400038, P.R. China.
authors.forename: Jiang
authors.initials: J
authors.lastname: Zheng
authors.affiliation: Department of Pharmacology, College of Pharmacy, the Third Military Medical University, Chongqing 400038, PR China. Electronic address: zhouh64@163.com.
authors.forename: Hong
authors.initials: H
authors.lastname: Zhou
mesh_terms: D000818:Animals; D058491:Bacterial Load; D002432:Cecum / Q000601:surgery; D016207:Cytokines / Q000378:metabolism; D004195:Disease Models, Animal; D006801:Humans; D057131:Immune Evasion; D007165:Immunosuppression Therapy; D007249:Inflammation / Q000276:immunology*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D011552:Pseudomonas Infections / Q000276:immunology*; D011550:Pseudomonas aeruginosa / Q000276:immunology*; D018805:Sepsis / Q000276:immunology*
chemical_list: D016207:Cytokines
keywords: Bacterial load; Cecal ligation and puncture; Cytokines; Immunosuppression; Pseudomonas aeruginosa; Sepsis