title: Bacterial diversity shift determined by different diets in the gut of the spotted wing fly Drosophila suzukii is primarily reflected on acetic acid bacteria.
abstract: The pivotal role of diet in shaping gut microbiota has been evaluated in different animal models, including insects. Drosophila flies harbour an inconstant microbiota among which acetic acid bacteria (AAB) are important components. Here, we investigated the bacterial and AAB components of the invasive pest Drosophila suzukii microbiota, by studying the same insect population separately grown on fruit-based or non-fruit artificial diet. AAB were highly prevalent in the gut under both diets (90 and 92% infection rates with fruits and artificial diet respectively). Fluorescent in situ hybridization and recolonization experiments with green fluorescent protein (Gfp)-labelled strains showed AAB capability to massively colonize insect gut. High-throughput sequencing on 16S rRNA gene indicated that the bacterial microbiota of guts fed with the two diets clustered separately. By excluding AAB-related OTUs from the analysis, insect bacterial communities did not cluster separately according to the diet, suggesting that diet-based diversification of the community is primarily reflected on the AAB component of the community. Diet influenced also AAB alpha-diversity, with separate OTU distributions based on diets. High prevalence, localization and massive recolonization, together with AAB clustering behaviour in relation to diet, suggest an AAB role in the D. suzukii gut response to diet modification.
authors.affiliation: Dipartimento di Scienze per gli Alimenti, la Nutrizione e l'Ambiente (DeFENS), Università degli Studi di Milano, Milano, Italy.
authors.forename: Violetta
authors.initials: V
authors.lastname: Vacchini
authors.affiliation: Dipartimento di Scienze Agrarie, Forestali e Alimentari (DISAFA), Università degli Studi di Torino, Grugliasco, Italy.
authors.forename: Elena
authors.initials: E
authors.lastname: Gonella
authors.affiliation: Dipartimento di Scienze per gli Alimenti, la Nutrizione e l'Ambiente (DeFENS), Università degli Studi di Milano, Milano, Italy.
authors.forename: Elena
authors.initials: E
authors.lastname: Crotti
authors.affiliation: Dipartimento di Scienze per gli Alimenti, la Nutrizione e l'Ambiente (DeFENS), Università degli Studi di Milano, Milano, Italy.
authors.forename: Erica M
authors.initials: EM
authors.lastname: Prosdocimi
authors.affiliation: Dipartimento di Scienze Agrarie, Forestali e Alimentari (DISAFA), Università degli Studi di Torino, Grugliasco, Italy.
authors.forename: Fabio
authors.initials: F
authors.lastname: Mazzetto
authors.affiliation: Dipartimento di Scienze per gli Alimenti, la Nutrizione e l'Ambiente (DeFENS), Università degli Studi di Milano, Milano, Italy.
authors.forename: Bessem
authors.initials: B
authors.lastname: Chouaia
authors.affiliation: Dipartimento di Scienze per gli Alimenti, la Nutrizione e l'Ambiente (DeFENS), Università degli Studi di Milano, Milano, Italy.
authors.forename: Matteo
authors.initials: M
authors.lastname: Callegari
authors.affiliation: Dipartimento di Scienze per gli Alimenti, la Nutrizione e l'Ambiente (DeFENS), Università degli Studi di Milano, Milano, Italy.
authors.forename: Francesca
authors.initials: F
authors.lastname: Mapelli
authors.affiliation: Dipartimento di Scienze della Vita (DSV), Università degli Studi di Modena e Reggio Emilia, Modena, Italy.
authors.forename: Mauro
authors.initials: M
authors.lastname: Mandrioli
authors.affiliation: Dipartimento di Scienze Agrarie, Forestali e Alimentari (DISAFA), Università degli Studi di Torino, Grugliasco, Italy.
authors.forename: Alberto
authors.initials: A
authors.lastname: Alma
authors.affiliation: Dipartimento di Scienze per gli Alimenti, la Nutrizione e l'Ambiente (DeFENS), Università degli Studi di Milano, Milano, Italy.|Biological and Environmental Sciences and Engineering Division (BESE), King Abdullah University of Science and Technology (KAUST), Thuwal, 23955-6900, Kingdom of Saudi Arabia.
authors.forename: Daniele
authors.initials: D
authors.lastname: Daffonchio
mesh_terms: D000818:Animals; D001419:Bacteria / Q000145:classification* / Q000187:drug effects* / Q000235:genetics; D058448:Biota / Q000187:drug effects*; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D004032:Diet; D004330:Drosophila / Q000382:microbiology* / Q000502:physiology*; D041981:Gastrointestinal Tract / Q000382:microbiology; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S