title: Competitive adsorption of fibronectin and albumin on hydroxyapatite nanocrystals.
abstract: Competitive adsorption of two-component solutions containing fibronectin (Fn) and albumin (Ab) on hydroxyapatite (HAp) nanocrystals was analyzedin situusing the quartz crystal microbalance with dissipation (QCM-D) technique. Adsorption of the one-component protein (Fn or Ab) and the two-component proteins adjusted to different molar ratios of Fn to Ab at a fixed Fn concentration was investigated. The frequency shift (Δf; Hz) and the dissipation energy shift (ΔD) were measured with the QCM-D technique, and the viscoelastic changes of adlayers were evaluated by the saturated ΔD/Δfvalue and the Voigt-based viscoelastic model. For the adsorption of the one-component protein, the Fn adlayer showed a larger mass and higher viscoelasticity than the Ab adlayer, indicating the higher affinity of Fn on HAp. For the adsorption of the two-component proteins, the viscoelastic properties of the adlayers became elastic with increase in Ab concentration, whereas the adsorption mass was similar to that of Fn in the one-component solution regardless of the Ab concentration. The specific binding mass of the Ab antibody to the adlayers increased with increase in Ab concentration, whereas that of the Fn antibody decreased. Therefore, Fn preferentially adsorbs on HAp and Ab subsequently interacts with the adlayers, indicating that the interfacial viscoelasticity of the adlayers was dominated by the interaction between Fn and Ab.
authors.affiliation: Department of Metallurgy and Ceramics Science, Tokyo Institute of Technology, Tokyo 152-8550, Japan; Biomaterials Center, National Institute for Materials Science, Tsukuba, Ibaraki 305-0047, Japan; Research Fellow of the Japan Society for the Promotion of Science, Tokyo, Japan.
authors.forename: Motohiro
authors.initials: M
authors.lastname: Tagaya
authors.affiliation: Department of Metallurgy and Ceramics Science, Tokyo Institute of Technology, Tokyo 152-8550, Japan; Biomaterials Center, National Institute for Materials Science, Tsukuba, Ibaraki 305-0047, Japan.
authors.forename: Toshiyuki
authors.initials: T
authors.lastname: Ikoma
authors.affiliation: Biomaterials Center, National Institute for Materials Science, Tsukuba, Ibaraki 305-0047, Japan.
authors.forename: Nobutaka
authors.initials: N
authors.lastname: Hanagata
authors.affiliation: Department of Metallurgy and Ceramics Science, Tokyo Institute of Technology, Tokyo 152-8550, Japan.
authors.forename: Tomohiko
authors.initials: T
authors.lastname: Yoshioka
authors.affiliation: Department of Metallurgy and Ceramics Science, Tokyo Institute of Technology, Tokyo 152-8550, Japan.
authors.forename: Junzo
authors.initials: J
authors.lastname: Tanaka
keywords: QCM-D; competitive adsorption; hydroxyapatite; soft interface; viscoelastic property