title: Anti-Herpes Simplex Virus Type-1 Activity and Phenolic Content of Crude Ethanol Extract and Four Corresponding Fractions of Quercus brantii L Acorn.
abstract: This research was aimed to evaluate anti-herpes simplex virus type-1 (anti-HSV-1) activity of crude ethanol extract and 4 corresponding fractions of Quercus brantii acorn in vitro. Crude ethanol extract was prepared and subjected to fractionation with different polarity. Anti-HSV-1 activity was evaluated on baby hamster kidney cell line using MTT assay. The inhibitory effect of the plant materials on adsorption and/or post-adsorption stages of HSV-1 replication cycle were determined. Regression analysis was used to determine 50% inhibitory concentration and 50% cytotoxicity concentration, from which selective index was calculated. Based on our results, the chloroform fraction and the crude extract had the highest effect against HSV-1 with selectivity indices of 53.8 and 48.4, respectively. The n-hexane, n-butanol, and chloroform fractions inhibited HSV-1 replication in postadsorption stage ( P < .001). The results obtained indicated that the chloroform fraction of Q brantii acorn with high inhibitory effect against HSV-1 replication could be a new promising anti-HSV-1 agent.
authors.affiliation: 1 Medical Plants Research Center, Shahrekord University of Medical Science, Shahrekord, Iran.
authors.forename: Ali
authors.initials: A
authors.lastname: Karimi
authors.affiliation: 1 Medical Plants Research Center, Shahrekord University of Medical Science, Shahrekord, Iran.
authors.forename: Mahmoud
authors.initials: M
authors.lastname: Rafieian-Kopaei
authors.affiliation: 2 Students Research Committee, Shahrekord University of Medical Science, Shahrekord, Iran.
authors.forename: Mohammad-Taghi
authors.initials: MT
authors.lastname: Moradi
authors.affiliation: 3 Cellular and Molecular Research Center; Shahrekord University of Medical Science, Shahrekord, Iran.
authors.forename: Somayeh
authors.initials: S
authors.lastname: Alidadi
mesh_terms: D000818:Animals; D000998:Antiviral Agents / Q000494:pharmacology*; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D006224:Cricetinae; D005419:Flavonoids / Q000032:analysis; D018259:Herpesvirus 1, Human / Q000187:drug effects*; D010636:Phenols / Q000032:analysis*; D010936:Plant Extracts / Q000032:analysis / Q000494:pharmacology*; D029963:Quercus / Q000737:chemistry*
chemical_list: D000998:Antiviral Agents; D005419:Flavonoids; D010636:Phenols; D010936:Plant Extracts
keywords: Quercus brantii; antiviral; herpes simplex virus