title: Multilocus Sequence Typing Analysis of Carbapenem-ResistantAcinetobacter baumanniiin a Chinese Burns Institute.
abstract: Acinetobacter baumanniiis a leading pathogen responsible for nosocomial infections. The emergence of carbapenem-resistantA. baumannii(CRAB) has left few effective antibiotics for clinicians to use. To investigate the temporal evolutionary relationships among CRAB strains, we collected 248 CRAB isolates from a Chinese burns institute over 3 years. The prevalence of theOXA-23gene was detected by polymerase chain reaction. Multilocus sequence typing was used to type the CRAB strains and eBURST was used to analyze their evolutionary relationships. Wound surfaces (41%), sputa (24%), catheters (15%), and bloods (14%) were the four dominant isolation sources. Except for minocycline (33.5%) and sulbactam/cefoperazone (74.6%), these CRAB strains showed high resistance rates (>90%) to 16 tested antibiotics. The 248 isolates fall into 26 sequence types (STs), including nine known STs and 17 unknown STs. The majority (230/248) of these isolates belong to clonal complex 92 (CC92), including eight isolates belonging to seven unreported STs. A new CC containing 11 isolates grouped into four new STs was identified. TheOXA-23gene was detected at high prevalence among the CRAB isolates and the prevalence rate among the various STs differed. The majority of the isolates displayed a close evolutionary relationship, suggesting that serious nosocomial spreading and nosocomial infections of CRAB have occurred in the burn unit. In conclusion, the main CC for CRAB in this Chinese burn unit remained unchanged during the 3-year study period, and a new CC was identified. CC92 was the dominant complex, and more attention should be directed toward monitoring the new CC we have identified herein.
authors.affiliation: State Key Laboratory of Trauma, Burns and Combined Injury, Institute of Burn Research, Southwest Hospital, Third Military Medical University Chongqing, China.
authors.forename: Guangtao
authors.initials: G
authors.lastname: Huang
authors.affiliation: State Key Laboratory of Trauma, Burns and Combined Injury, Institute of Burn Research, Southwest Hospital, Third Military Medical University Chongqing, China.
authors.forename: Supeng
authors.initials: S
authors.lastname: Yin
authors.affiliation: State Key Laboratory of Trauma, Burns and Combined Injury, Institute of Burn Research, Southwest Hospital, Third Military Medical University Chongqing, China.
authors.forename: Yali
authors.initials: Y
authors.lastname: Gong
authors.affiliation: Department of Microbiology, Bioinformatic Center, College of Basic Medical Sciences, Third Military Medical University Chongqing, China.
authors.forename: Xia
authors.initials: X
authors.lastname: Zhao
authors.affiliation: Department of Microbiology, Bioinformatic Center, College of Basic Medical Sciences, Third Military Medical University Chongqing, China.
authors.forename: Lingyun
authors.initials: L
authors.lastname: Zou
authors.affiliation: State Key Laboratory of Trauma, Burns and Combined Injury, Institute of Burn Research, Southwest Hospital, Third Military Medical University Chongqing, China.
authors.forename: Bei
authors.initials: B
authors.lastname: Jiang
authors.affiliation: State Key Laboratory of Trauma, Burns and Combined Injury, Institute of Burn Research, Southwest Hospital, Third Military Medical University Chongqing, China.
authors.forename: Zhiwei
authors.initials: Z
authors.lastname: Dong
authors.affiliation: State Key Laboratory of Trauma, Burns and Combined Injury, Institute of Burn Research, Southwest Hospital, Third Military Medical University Chongqing, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Trauma, Burns and Combined Injury, Institute of Burn Research, Southwest Hospital, Third Military Medical University Chongqing, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Chen
authors.affiliation: Department of Molecular Genetics and Microbiology, College of Medicine, University of Florida, Gainesville FL, USA.
authors.forename: Shouguang
authors.initials: S
authors.lastname: Jin
authors.affiliation: State Key Laboratory of Trauma, Burns and Combined Injury, Institute of Burn Research, Southwest Hospital, Third Military Medical University Chongqing, China.
authors.forename: Zhiqiang
authors.initials: Z
authors.lastname: Yuan
authors.affiliation: State Key Laboratory of Trauma, Burns and Combined Injury, Institute of Burn Research, Southwest Hospital, Third Military Medical University Chongqing, China.
authors.forename: Yizhi
authors.initials: Y
authors.lastname: Peng
keywords: A. baumannii; clonal complex (CC); multi locus sequence typing (MLST); nosocomial infection; β-lactamases