title: Determination of patulin in apple juice using magnetic solid-phase extraction coupled with high-performance liquid chromatography.
abstract: An efficient magnetic sorbent consisting of benzofuran-2-carboxylic acid-loaded magnetic nanocomposite was successfully synthesised for pre-concentration of patulin from apple juice. The prepared magnetic nanocomposite was characterised by scanning electron microscopy, transmission electron microscopy and Fourier-transform infrared spectroscopy. Determination of enriched patulin was performed by high-performance liquid chromatography. The best adsorption conditions were 40 mg of sorbent, 50 ml of apple juice sample, pH 5, ambient temperature and 25 min; the elution conditions were 500 μl methanol, pH 5, ambient temperature, and 4 min. Under optimised conditions, pre-concentration factor was 100, linearity range was 1-400 μg l-1of patulin, limit of detection was 0.15 μg l-1and limit of quantification was 0.5 μg l-1. When samples were determined 20 times, the recovery was 93.9-102.6% and the relative standard deviation was below 5.3%. In terms of proposed procedure, the developed method was successfully applied for patulin detection in apple juice samples.
authors.affiliation: a College of Chemistry and Materials , Shanxi Normal University , Linfen , China.
authors.forename: Youwei
authors.initials: Y
authors.lastname: Yu
authors.affiliation: a College of Chemistry and Materials , Shanxi Normal University , Linfen , China.
authors.forename: Zhefeng
authors.initials: Z
authors.lastname: Fan
mesh_terms: D001628:Beverages / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D060328:Magnetic Phenomena; D027845:Malus / Q000737:chemistry*; D010365:Patulin / Q000032:analysis*; D052616:Solid Phase Extraction
chemical_list: D010365:Patulin
keywords: Patulin; apple juice; high-performance liquid chromatography; magnetic solid-phase extraction