title: Effects of ozone nano-bubble water on periodontopathic bacteria and oral cells -in vitrostudies.
abstract: The aims of the present study were to evaluate the bactericidal activity of a new antiseptic agent, ozone nano-bubble water (NBW3), against periodontopathogenic bacteria and to assess the cytotoxicity of NBW3 against human oral cells. The bactericidal activities of NBW3 against representative periodontopathogenic bacteria,Porphyromonas gingivalis(P. gingivalis) andAggregatibacter actinomycetemcomitans(A. actinomycetemcomitans) were evaluated usingin vitrotime-kill assays. The cytotoxicity of NBW3 was evaluated using three-dimensional human buccal and gingival tissue models. The numbers of colony forming units (CFUs)/mL ofP. gingivalisandA. actinomycetemcomitansexposed to NBW3 dropped to below the lower limit of detection (<10 CFUs mL-1) after only 0.5 min of exposure. There were only minor decreases in the viability of oral tissue cells after 24 h of exposure to NBW3. These results suggest that NBW3 possesses potent bactericidal activity against representative periodontopathogenic bacteria and is not cytotoxic to cells of human oral tissues. The use of NBW3 as an adjunct to periodontal therapy would be promising.
authors.affiliation: Department of Periodontology, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo-ku, Tokyo, 113-8510, Japan.
authors.forename: Sae
authors.initials: S
authors.lastname: Hayakumo
authors.affiliation: Department of Lifetime Oral Health Care Science, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo-ku, Tokyo, 113-8510, Japan.
authors.forename: Shinichi
authors.initials: S
authors.lastname: Arakawa
authors.affiliation: National Institute of Advanced Industrial Science and Technology (AIST), 16-1 Onogawa, Tsukuba, Ibaraki, 305-8569, Japan.
authors.forename: Masayoshi
authors.initials: M
authors.lastname: Takahashi
authors.affiliation: Department of Lifetime Oral Health Care Science, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo-ku, Tokyo, 113-8510, Japan.
authors.forename: Keiko
authors.initials: K
authors.lastname: Kondo
authors.affiliation: Hyperbaric Medical Center, Hospital of Medicine, Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo-ku, Tokyo, 113-8510, Japan.
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Mano
authors.affiliation: Department of Periodontology, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo-ku, Tokyo, 113-8510, Japan; Global Center of Excellence (GCOE) Program, Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo-ku, Tokyo, 113-8510, Japan.
authors.forename: Yuichi
authors.initials: Y
authors.lastname: Izumi
keywords: antiseptic; cytotoxicity; ozone nano-bubble water; periodontitis; periodontopathic bacteria