title: Carbon Nanotubes Based Glucose Needle-type Biosensor.
abstract: A novel needle-type biosensor based on carbon nanotubes is reported. Thebiosensor was prepared by packing a mixture of multi-wall carbon nanotubes (MWCNTs),graphite powder and glucose oxidase (Gox) freeze-dried powder into a glass capillary of 0.5mm inner diameter. The resulting amperometric biosensor was characterizedelectrochemically using amperometry in the presence of hydrogen peroxide and in thepresence of glucose. The glucose biosensor sensitivity was influenced by the glucoseoxidase concentration within the MWCNTs mixture. The optimized glucose needle-typebiosensor displayed better sensitivity and stability, and a detected range of up to 20 mM.Based on its favorable stability, the needle biosensor was first time used in real-timemonitoring system as a kind of online glucose detector. The decay of current response isless than 10% after 24-hour continuous observation.
authors.affiliation: Zhejiang University, College of Life Sciences, HangZhou 310027, P.R. China. jia_zq9803@hotmail.com.|GroepT Leuven Hogeschool, Vesaliusstraat 13, B-3000 Leuven, Belgium. jia_zq9803@hotmail.com.
authors.forename: Jinyan
authors.initials: J
authors.lastname: Jia
authors.affiliation: Zhejiang University, College of Life Sciences, HangZhou 310027, P.R. China. guanwj@zju.edu.cn.
authors.forename: Wenjun
authors.initials: W
authors.lastname: Guan
authors.affiliation: Zhejiang University, College of Life Sciences, HangZhou 310027, P.R. China. benalishhao@hotmail.com.
authors.forename: Minghao
authors.initials: M
authors.lastname: Sim
authors.affiliation: Zhejiang University, College of Life Sciences, HangZhou 310027, P.R. China. lyq@zju.edu.cn.
authors.forename: Yongquan
authors.initials: Y
authors.lastname: Li
authors.affiliation: Zhejiang First Hospital, HangZhou 31003, P.R. China. lihong@mail.hz.zj.cn.
authors.forename: Hong
authors.initials: H
authors.lastname: Li
keywords: Multiwall carbon nanotubes; glucose; needle-type biosensor