title: Assessing the 5S ribosomal RNA heterogeneity in Arabidopsis thaliana using short RNA next generation sequencing data.
abstract: In eukaryotes, ribosomal 5S rRNAs are products of multigene families organized within clusters of tandemly repeated units. Accumulation of genomic data obtained from a variety of organisms demonstrated that the potential 5S rRNA coding sequences show a large number of variants, often incompatible with folding into a correct secondary structure. Here, we present results of an analysis of a large set of short RNA sequences generated by the next generation sequencing techniques, to address the problem of heterogeneity of the 5S rRNA transcripts in Arabidopsis and identification of potentially functional rRNA-derived fragments.
authors.affiliation: Department of Computational Biology, Institute of Molecular Biology and Biotechnology, Adam Mickiewicz University in Poznan, Poznań, Poland.
authors.forename: Maciej
authors.initials: M
authors.lastname: Szymanski
authors.affiliation: Department of Computational Biology, Institute of Molecular Biology and Biotechnology, Adam Mickiewicz University in Poznan, Poznań, Poland.
authors.forename: Wojciech M
authors.initials: WM
authors.lastname: Karlowski
mesh_terms: D017360:Arabidopsis / Q000235:genetics*; D001483:Base Sequence; D015870:Gene Expression; D059014:High-Throughput Nucleotide Sequencing; D055029:Inverted Repeat Sequences; D018749:RNA, Plant / Q000235:genetics*; D012341:RNA, Ribosomal, 5S / Q000235:genetics*; D017423:Sequence Analysis, RNA; D012689:Sequence Homology, Nucleic Acid
chemical_list: D018749:RNA, Plant; D012341:RNA, Ribosomal, 5S