title: Basic Fibroblast Growth Factor Regulates REX1 Expression Via IL-6 In Stem Cells Isolated From Human Exfoliated Deciduous Teeth.
abstract: Basic fibroblast growth factor (bFGF) regulates pluripotent marker expression and cellular differentiation in various cell types. However, the mechanism by which bFGF regulates REX1 expression in stem cells, isolated from human exfoliated deciduous teeth (SHEDs) remains unclear. The aim of the present study was to investigate the regulation of REX1 expression by bFGF in SHEDs. SHEDs were isolated and characterized. Their mRNA and protein expression levels were determined using real-time polymerase chain reaction and enzyme-linked immunosorbent assay, respectively. In some experiments, chemical inhibitors were added to the culture medium to impede specific signaling pathways. Cells isolated from human exfoliated deciduous tooth dental pulp tissue expressed mesenchymal stem cell surface markers (CD44, CD73, CD90, and CD105). These cells differentiated into osteogenic and adipogenic lineages, when appropriately induced. Treating SHEDs with bFGF induced REX1 mRNA expression and this effect was attenuated by pretreatment with FGFR or Akt inhibitors. Cycloheximide pretreatment also inhibited the bFGF-induced REX1 expression, implying the involvement of intermediate molecule(s). Further, the addition of an IL-6 neutralizing antibody attenuated the bFGF-induced REX1 expression by SHEDs. In conclusion, bFGF enhanced REX1 expression by SHEDs via the FGFR and Akt signaling pathways. Moreover, IL-6 participated in the bFGF-induced REX1 expression in SHEDs. J. Cell. Biochem. 118: 1480-1488, 2017. © 2016 Wiley Periodicals, Inc.
authors.affiliation: Graduate Program in Oral Biology, Faculty of Dentistry, Chulalongkorn University, Bangkok, 10330, Thailand.|Mineralized Tissue Research Unit, Faculty of Dentistry, Chulalongkorn University, Bangkok, 10330, Thailand.
authors.forename: Nunthawan
authors.initials: N
authors.lastname: Nowwarote
authors.affiliation: Department of Pediatric Dentistry, Faculty of Dentistry, Chulalongkorn University, Bangkok, 10330, Thailand.
authors.forename: Waleerat
authors.initials: W
authors.lastname: Sukarawan
authors.affiliation: Mineralized Tissue Research Unit, Faculty of Dentistry, Chulalongkorn University, Bangkok, 10330, Thailand.|Department of Anatomy, Faculty of Dentistry, Chulalongkorn University, Bangkok, 10330, Thailand.
authors.forename: Prasit
authors.initials: P
authors.lastname: Pavasant
authors.affiliation: Mineralized Tissue Research Unit, Faculty of Dentistry, Chulalongkorn University, Bangkok, 10330, Thailand.|Department of Anatomy, Faculty of Dentistry, Chulalongkorn University, Bangkok, 10330, Thailand.
authors.forename: Thanaphum
authors.initials: T
authors.lastname: Osathanon
mesh_terms: D002454:Cell Differentiation; D049109:Cell Proliferation; D002478:Cells, Cultured; D002648:Child; D005260:Female; D016222:Fibroblast Growth Factor 2 / Q000378:metabolism*; D005346:Fibroblast Growth Factors / Q000235:genetics; D005786:Gene Expression Regulation; D006801:Humans; D015850:Interleukin-6 / Q000235:genetics*; D051741:Kruppel-Like Transcription Factors / Q000235:genetics* / Q000378:metabolism; D008297:Male; D051057:Proto-Oncogene Proteins c-akt / Q000235:genetics; D015398:Signal Transduction; D013234:Stem Cells / Q000166:cytology* / Q000378:metabolism; D014080:Tooth Exfoliation / Q000235:genetics / Q000378:metabolism; D014094:Tooth, Deciduous / Q000166:cytology* / Q000378:metabolism
chemical_list: C508600:IL6 protein, human; D015850:Interleukin-6; D051741:Kruppel-Like Transcription Factors; C507976:ZFP42 protein, human; D016222:Fibroblast Growth Factor 2; D005346:Fibroblast Growth Factors; D051057:Proto-Oncogene Proteins c-akt
keywords: BASIC FIBROBLAST GROWTH FACTOR; INTERLEUKIN-6; REX-1; STEM CELLS ISOLATED FROM HUMAN EXFOLIATED DECIDUOUS TEETH