title: Comparison of two freely available software packages for mass spectrometry imaging data analysis using brains from morphine addicted rats.
abstract: Data analysis from mass spectrometry imaging (MSI) imaging experiments is a very complex task. Most of the software packages devoted to this purpose are designed by the mass spectrometer manufacturers and, thus, are not freely available. Laboratories developing their own MS-imaging sources usually do not have access to the commercial software, and they must rely on the freely available programs. The most recognized ones are BioMap, developed by Novartis under Interactive Data Language (IDL), and Datacube, developed by the Dutch Foundation for Fundamental Research of Matter (FOM-Amolf). These two systems were used here for the analysis of images received from rat brain tissues subjected to morphine influence and their capabilities were compared in terms of ease of use and the quality of obtained results.
authors.affiliation: AGH University of Science and Technology, Faculty of Materials Science and Ceramics, Department of Biochemistry and Neurobiology, Mickiewicza 30, 30-059 Cracow, Poland. abk@agh.edu.pl.
authors.forename: Anna
authors.initials: A
authors.lastname: Bodzon-Kulakowska
authors.affiliation: Department of Pharmacology and Pharmacodynamics, Medical University, Chodzki 4a, 20-093 Lublin, Poland.
authors.forename: Marta
authors.initials: M
authors.lastname: Marszalek-Grabska
authors.affiliation: AGH University of Science and Technology, Faculty of Materials Science and Ceramics, Department of Biochemistry and Neurobiology, Mickiewicza 30, 30-059 Cracow, Poland.
authors.forename: Anna
authors.initials: A
authors.lastname: Antolak
authors.affiliation: AGH University of Science and Technology, Faculty of Materials Science and Ceramics, Department of Biochemistry and Neurobiology, Mickiewicza 30, 30-059 Cracow, Poland.
authors.forename: Anna
authors.initials: A
authors.lastname: Drabik
authors.affiliation: Department of Pharmacology and Pharmacodynamics, Medical University, Chodzki 4a, 20-093 Lublin, Poland.
authors.forename: Jolanta H
authors.initials: JH
authors.lastname: Kotlinska
authors.affiliation: AGH University of Science and Technology, Faculty of Materials Science and Ceramics, Department of Biochemistry and Neurobiology, Mickiewicza 30, 30-059 Cracow, Poland.
authors.forename: Piotr
authors.initials: P
authors.lastname: Suder
mesh_terms: D000465:Algorithms; D000818:Animals; D001921:Brain / Q000378:metabolism*; D013058:Mass Spectrometry / Q000379:methods*; D057054:Molecular Imaging / Q000379:methods*; D009021:Morphine Dependence / Q000378:metabolism*; D009419:Nerve Tissue Proteins / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012984:Software; D046888:Tissue Array Analysis / Q000379:methods*; D014018:Tissue Distribution
chemical_list: D009419:Nerve Tissue Proteins