title: Semi-industrial scale (30 m3) fed-batch fermentation for the production of D-lactate by Escherichia coli strain HBUT-D15.
abstract: D(-)-lactic acid is needed for manufacturing of stereo-complex poly-lactic acid polymer. Large scale D-lactic acid fermentation, however, has yet to be demonstrated. A genetically engineered Escherichia coli strain, HBUT-D, was adaptively evolved in a 15% calcium lactate medium for improved lactate tolerance. The resulting strain, HBUT-D15, was tested at a lab scale (7 L) by fed-batch fermentation with up to 200 g L-1of glucose, producing 184-191 g L-1of D-lactic acid, with a volumetric productivity of 4.38 g L-1h-1, a yield of 92%, and an optical purity of 99.9%. The HBUT-D15 was then evaluated at a semi-industrial scale (30 m3) via fed-batch fermentation with up to 160 g L-1of glucose, producing 146-150 g L-1of D-lactic acid, with a volumetric productivity of 3.95-4.29 g L-1h-1, a yield of 91-94%, and an optical purity of 99.8%. These results are comparable to that of current industrial scale L(+)-lactic acid fermentation.
authors.affiliation: Key Laboratory of Fermentation Engineering (Ministry of Education), Hubei Provincial Cooperative Innovation Center of Industrial Fermentation, College of Bioengineering, Hubei University of Technology, Wuhan, 430068, People's Republic of China.
authors.forename: Xiangmin
authors.initials: X
authors.lastname: Fu
authors.affiliation: Key Laboratory of Fermentation Engineering (Ministry of Education), Hubei Provincial Cooperative Innovation Center of Industrial Fermentation, College of Bioengineering, Hubei University of Technology, Wuhan, 430068, People's Republic of China.
authors.forename: Yongze
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Key Laboratory of Fermentation Engineering (Ministry of Education), Hubei Provincial Cooperative Innovation Center of Industrial Fermentation, College of Bioengineering, Hubei University of Technology, Wuhan, 430068, People's Republic of China. wangjinhua@mail.hbut.edu.cn.
authors.forename: Jinhua
authors.initials: J
authors.lastname: Wang
authors.affiliation: Department of Biological Sciences, Northern Illinois University, DeKalb, IL, 60115, USA.
authors.forename: Erin
authors.initials: E
authors.lastname: Garza
authors.affiliation: Department of Biological Sciences, Northern Illinois University, DeKalb, IL, 60115, USA.
authors.forename: Ryan
authors.initials: R
authors.lastname: Manow
authors.affiliation: Department of Biological Sciences, Northern Illinois University, DeKalb, IL, 60115, USA. szhou@niu.edu.
authors.forename: Shengde
authors.initials: S
authors.lastname: Zhou
mesh_terms: D061249:Batch Cell Culture Techniques; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D019143:Evolution, Molecular; D005285:Fermentation; D005947:Glucose / Q000378:metabolism; D007218:Industrial Microbiology; D019344:Lactic Acid / Q000096:biosynthesis*; D000074041:Microorganisms, Genetically-Modified
chemical_list: D019344:Lactic Acid; D005947:Glucose
keywords: D-lactic acid; E. coli; Fed-batch fermentation; Polylactic acid polymer; Scale-up fermentation