title: Long-term persistence in protection and response to a hepatitis B vaccine booster among adolescents immunized in infancy in the western region of China.
abstract: To evaluate the persistence of protection from hepatitis B (HB) vaccination among adolescents immunized with a primary series of HB vaccine as infants, and the immune response to booster doses.
  
  
  
  Healthy adolescents aged 15-17 y vaccinated with HB vaccine only at birth were enrolled. Baseline serum hepatitis B surface antigen (HBsAg), antibody against hepatitis B surface antigen (anti-HBs) and antibody against hepatitis B core antigen (anti-HBc) were detected by Enzyme-Linked Immunosorbent Assay (ELISA) and anti-HBs level was measured using Chemiluminescent Microparticle Immunoassay (CMIA). The rate of HBV infection was calculated. The seroprotection rate of anti-HBs (≥ 10 mIU/ml) and GMC level were used to evaluate the persistence of immunity from HB vaccination. Those with anti-HBs < 10 mIU/ml were immunized with booster doses of HB vaccine and the anamnestic response was assessed.
  
  
  
  Of 180 adolescents who received a primary series of HB vaccinations as infants, 3 (1.7%) had HBV infection and 74 (41.1%) had anti-HBs ≥ 10 mIU/ml with a GMC of 145.11 mIU/ml. The remaining 103 (57.2%) with anti-HBs < 10 mIU/ml received a booster dose of 20 μg HB vaccine and achieved the seroprotection rate of 84% (84/100) and a GMC of 875.19 mIU/ml at one month post-booster. An additional dose of 60 μg HB vaccine was administered to the 16 adolescents with anti-HBs < 10 mIU/ml after the first booster. All of them obtained anti-HBs seroprotection with a GMC of 271.02 mIU/ml at 1.5 months after an additional dose.
  
  
  
  Vaccine-induced immunity persisted for up to 15-17 y in 89.3% (158/177) of participants after a primary HB vaccination in infancy. Administering a booster dose of 20μg HB vaccine elicited an anamnestic immune responses in the majority of individuals with baseline anti-HBs <10 mIU/ml.
authors.affiliation: a Department of Microbiology and Infectious Disease Center , School of Basic Medical Sciences, Peking University Health Science Center , Beijing , China.
authors.forename: Zhen-Zi
authors.initials: ZZ
authors.lastname: Wang
authors.affiliation: a Department of Microbiology and Infectious Disease Center , School of Basic Medical Sciences, Peking University Health Science Center , Beijing , China.
authors.forename: Yu-Hua
authors.initials: YH
authors.lastname: Gao
authors.affiliation: a Department of Microbiology and Infectious Disease Center , School of Basic Medical Sciences, Peking University Health Science Center , Beijing , China.
authors.forename: Wei
authors.initials: W
authors.lastname: Lu
authors.affiliation: b Beijing 302 Hospital of People's Liberation Army , Beijing , China.
authors.forename: Cun-Duo
authors.initials: CD
authors.lastname: Jin
authors.affiliation: c Shenzhen Kangtai Biological Products Co., LTD. , Shenzhen , Guangdong , China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Zeng
authors.affiliation: a Department of Microbiology and Infectious Disease Center , School of Basic Medical Sciences, Peking University Health Science Center , Beijing , China.
authors.forename: Ling
authors.initials: L
authors.lastname: Yan
authors.affiliation: a Department of Microbiology and Infectious Disease Center , School of Basic Medical Sciences, Peking University Health Science Center , Beijing , China.
authors.forename: Feng
authors.initials: F
authors.lastname: Ding
authors.affiliation: a Department of Microbiology and Infectious Disease Center , School of Basic Medical Sciences, Peking University Health Science Center , Beijing , China.
authors.forename: Tong
authors.initials: T
authors.lastname: Li
authors.affiliation: a Department of Microbiology and Infectious Disease Center , School of Basic Medical Sciences, Peking University Health Science Center , Beijing , China.
authors.forename: Xue-En
authors.initials: XE
authors.lastname: Liu
authors.affiliation: a Department of Microbiology and Infectious Disease Center , School of Basic Medical Sciences, Peking University Health Science Center , Beijing , China.
authors.forename: Hui
authors.initials: H
authors.lastname: Zhuang
mesh_terms: D000293:Adolescent; D002681:China; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006509:Hepatitis B / Q000517:prevention & control*; D006510:Hepatitis B Antibodies / Q000097:blood*; D006512:Hepatitis B Core Antigens / Q000276:immunology; D006514:Hepatitis B Surface Antigens / Q000276:immunology; D017325:Hepatitis B Vaccines / Q000008:administration & dosage* / Q000276:immunology*; D006801:Humans; D007117:Immunization, Secondary; D008163:Luminescent Measurements; D008297:Male
chemical_list: D006510:Hepatitis B Antibodies; D006512:Hepatitis B Core Antigens; D006514:Hepatitis B Surface Antigens; D017325:Hepatitis B Vaccines
keywords: adolescents; booster vaccination; hepatitis B vaccine; immune memory; long-term immunogenicity