title: Evaluation of Hypotensive and Antihypertensive Effects of Velvet Bean (Mucuna pruriens L.) Hydrolysates.
abstract: Hypertension could cause significant worldwide health problems that affect 15-20% of all adults; according to National Health and Nutrition Examination Survey, about 29% of the adult population in the United States are hypertensive. Recent research has shown that peptides derived from the hydrolysis of food proteins can decrease blood pressure. This study was carried out to evaluate the hypotensive and antihypertensive potential of Mucuna pruriens protein hydrolysates in in vitro and in vivo models. M. pruriens protein concentrate was prepared by wet fractionation and enzymatically hydrolyzed using Alcalase®, Flavourzyme®, and the sequential system Alcalase-Flavourzyme at different times (5-120 min). The biological potential was measured in vitro based on the IC50value as well as in vivo effect, measuring the systolic (SBP) and diastolic (DBP) blood pressure in normotensive and antihypertensive Wistar-Kyoto rats by the tail-cuff method. Hydrolysis of M. pruriens protein concentrates with commercial enzymes generated extensive hydrolysates with angiotensin-converting enzyme (ACE-I) inhibitory activity (IC50: 0.589-0.993 mg/mL) and hypotensive (SBP: 0.6-47.43%, DBP: 1.94-43.47%) and antihypertensive (SBP: 8.84-27.29% DBP: 16.1-29.37%) effect. These results indicate that Mucuna pruriens protein hydrolysate (MPPH) could be used as a functional ingredient to prevent blood pressure increase.
authors.affiliation: 1 Facultad de Ingeniería Química, Universidad Autónoma de Yucatán , Mérida, México .
authors.forename: Luis
authors.initials: L
authors.lastname: Chel-Guerrero
authors.affiliation: 1 Facultad de Ingeniería Química, Universidad Autónoma de Yucatán , Mérida, México .
authors.forename: Saulo
authors.initials: S
authors.lastname: Galicia-Martínez
authors.affiliation: 2 Facultad de Medicina, Universidad Autónoma del Estado de Morelos , Cuernavaca, México .
authors.forename: Juan José
authors.initials: JJ
authors.lastname: Acevedo-Fernández
authors.affiliation: 2 Facultad de Medicina, Universidad Autónoma del Estado de Morelos , Cuernavaca, México .
authors.forename: Jesus
authors.initials: J
authors.lastname: Santaolalla-Tapia
authors.affiliation: 1 Facultad de Ingeniería Química, Universidad Autónoma de Yucatán , Mérida, México .
authors.forename: David
authors.initials: D
authors.lastname: Betancur-Ancona
mesh_terms: D000806:Angiotensin-Converting Enzyme Inhibitors / Q000008:administration & dosage* / Q000737:chemistry; D000818:Animals; D000959:Antihypertensive Agents / Q000008:administration & dosage* / Q000737:chemistry; D001794:Blood Pressure / Q000187:drug effects; D004353:Drug Evaluation, Preclinical; D006801:Humans; D006973:Hypertension / Q000188:drug therapy* / Q000201:enzymology / Q000503:physiopathology; D031299:Mucuna / Q000737:chemistry*; D007703:Peptidyl-Dipeptidase A / Q000378:metabolism; D010936:Plant Extracts / Q000008:administration & dosage*; D011492:Protein Hydrolysates / Q000008:administration & dosage* / Q000737:chemistry; D051381:Rats; D011921:Rats, Inbred WKY; D012639:Seeds / Q000737:chemistry
chemical_list: D000806:Angiotensin-Converting Enzyme Inhibitors; D000959:Antihypertensive Agents; D010936:Plant Extracts; D011492:Protein Hydrolysates; D007703:Peptidyl-Dipeptidase A
keywords: ACE-I; Mucuna pruriens; antihypertensive; hypotensive; protein hydrolysates