title: Predictors of the Size of the Exchangeable Zinc Pool Differ between Children and Adults.
abstract: The size of the rapidly exchanging pool of body zinc has been suggested as having potential utility as a biomarker of zinc status. Knowledge of the relations of exchangeable zinc pool (EZP) size to relevant variables is necessary to adequately evaluate its use as a biomarker.
  
  
  
  We used regression analysis to investigate associations of EZP with age, sex, body size, and zinc nutrition variables.
  
  
  
  Data were compiled from 18 isotope tracer studies of zinc absorption in 247 children and adults (248 observations) in which EZP and relevant variables (e.g., weight, age, absorbed zinc) were measured. Linear regression analyses were performed separately on data from adults and children.
  
  
  
  In children, EZP was most strongly associated with weight (r = 0.78). The best-fitting regression models of EZP (R2≥ 0.68) had weight or age and weight-for-age z score as predictors. Other variables had little effect on EZP when controlling for weight. Absorbed zinc was observed to be a predictor of EZP only in zinc intervention trials of infants. The mean EZP/wt was 4 mg/kg between 8 and 120 mo of age. In adults, EZP was observed to vary in a complex manner with (in order of importance) age, absorbed zinc, weight, sex, and plasma zinc concentration. EZP data from zinc-deprived subjects were lower than the 95% prediction interval of a model of normative data.
  
  
  
  EZP was observed to maintain a constant size relative to weight and was influenced only slightly by other factors in children. In contrast, EZP in adults varied with several factors, including absorbed zinc, and was statistically smaller in zinc-deprived individuals. The findings suggest that EZP may have utility as an indicator of zinc status in adults, but there is less evidence for this in children. Additional data are needed to reach a definitive conclusion.
authors.affiliation: University of Colorado School of Medicine, Aurora, CO; and leland.miller@ucdenver.edu.
authors.forename: Leland V
authors.initials: LV
authors.lastname: Miller
authors.affiliation: University of Colorado School of Medicine, Aurora, CO; and.
authors.forename: K Michael
authors.initials: KM
authors.lastname: Hambidge
authors.affiliation: Children's Hospital Oakland Research Institute, Oakland, CA.
authors.forename: Janet C
authors.initials: JC
authors.lastname: King
authors.affiliation: University of Colorado School of Medicine, Aurora, CO; and.
authors.forename: Jamie E
authors.initials: JE
authors.lastname: Westcott
authors.affiliation: University of Colorado School of Medicine, Aurora, CO; and.
authors.forename: Nancy F
authors.initials: NF
authors.lastname: Krebs
mesh_terms: D000328:Adult; D000375:Aging; D049628:Body Size; D002648:Child; D005260:Female; D006801:Humans; D008297:Male; D008954:Models, Biological; D012044:Regression Analysis; D015032:Zinc / Q000378:metabolism*
chemical_list: D015032:Zinc
keywords: EZP; exchangeable zinc pool; regression analysis; zinc; zinc status