title: Concerted Changes in the Phosphoproteome and Metabolome Under Different CO2/O2 Gaseous Conditions in Arabidopsis Rosettes.
abstract: Considerable efforts are currently devoted to understanding the regulation of primary carbon metabolism in plant leaves, which is known to change dramatically with environmental conditions, e.g. during light/dark transitions. Protein phosphorylation is believed to be a key factor in such a metabolic control. In fact, some studies have suggested modifications in the phosphorylation status of key enzymes in the dark compared with the light, or when photosynthesis varies. However, a general view of the phosphoproteome and reciprocal alterations in both the phosphoproteome and metabolome under a wide spectrum of CO2and O2conditions so as to vary both gross photosynthesis and photorespiration is currently lacking. Here, we used an instant sampling system and strictly controlled gaseous conditions to examine short-term metabolome and phosphoproteome changes in Arabidopsis rosettes. We show that light/dark, CO2and O2mole fraction have differential effects on enzyme phosphorylation. Phosphorylation events that appear to be the most important to regulate metabolite contents when photosynthesis varies are those associated with sugar and pyruvate metabolism: sucrose and starch synthesis are major phosphorylation-controlled steps but pyruvate utilization (by phosphoenolpyruvate carboxylase and pyruvate dehydrogenase) and pyruvate reformation (by pyruvate orthophosphate dikinase) are also subjected to phosphorylation control. Our results thus show that the phosphoproteome response to light/dark transition and gaseous conditions (CO2, O2) contributes to the rapid adjustment of major pathways of primary C metabolism.
authors.affiliation: Research School of Biology, College of Medicine, Biology and Environment, Australian National University, Canberra ACT 2601, Australia.|These authors contributed equally to this work.
authors.forename: Cyril
authors.initials: C
authors.lastname: Abadie
authors.affiliation: Institute of Plant Sciences Paris-Saclay, UMR 9213/UMR1403, Université Paris Sud, CNRS-INRA, Université d'Evry, Université Paris-Diderot, Bâtiment 630, 91405 Orsay Cedex, France.|These authors contributed equally to this work.
authors.forename: Samuel
authors.initials: S
authors.lastname: Mainguet
authors.affiliation: Plateforme PAPPSO, UMR 0320/8120 Génétique Quantitative et Evolution, Université Paris-Sud, CNRS-INRA-AgroParisTech, Le Moulon, 91190 Gif-sur-Yvette, France.
authors.forename: Marlène
authors.initials: M
authors.lastname: Davanture
authors.affiliation: Institute of Plant Sciences Paris-Saclay, UMR 9213/UMR1403, Université Paris Sud, CNRS-INRA, Université d'Evry, Université Paris-Diderot, Bâtiment 630, 91405 Orsay Cedex, France.
authors.forename: Michael
authors.initials: M
authors.lastname: Hodges
authors.affiliation: Plateforme PAPPSO, UMR 0320/8120 Génétique Quantitative et Evolution, Université Paris-Sud, CNRS-INRA-AgroParisTech, Le Moulon, 91190 Gif-sur-Yvette, France.
authors.forename: Michel
authors.initials: M
authors.lastname: Zivy
authors.affiliation: Research School of Biology, College of Medicine, Biology and Environment, Australian National University, Canberra ACT 2601, Australia guillaume.tcherkez@anu.edu.au.
authors.forename: Guillaume
authors.initials: G
authors.lastname: Tcherkez
mesh_terms: D017360:Arabidopsis / Q000187:drug effects / Q000378:metabolism*; D002244:Carbon / Q000378:metabolism; D002245:Carbon Dioxide / Q000494:pharmacology*; D055442:Metabolome / Q000187:drug effects*; D015999:Multivariate Analysis; D009584:Nitrogen / Q000378:metabolism; D010100:Oxygen / Q000494:pharmacology*; D010748:Phosphopeptides / Q000378:metabolism; D010750:Phosphoproteins / Q000378:metabolism*; D010788:Photosynthesis / Q000187:drug effects; D018515:Plant Leaves / Q000187:drug effects / Q000378:metabolism*; D020543:Proteome / Q000378:metabolism*; D040901:Proteomics
chemical_list: D010748:Phosphopeptides; D010750:Phosphoproteins; D020543:Proteome; D002245:Carbon Dioxide; D002244:Carbon; D009584:Nitrogen; D010100:Oxygen
keywords: Catabolism; Metabolomics; Photosynthesis; Protein phosphorylation