title: Using crosslinkable diacetylene phospholipids to construct two-dimensional packed beds in supported lipid bilayer separation platforms.
abstract: Separating and purifying cell membrane-associated biomolecules has been a challenge owing to their amphiphilic property. Taking these species out of their native lipid membrane environment usually results in biomolecule degradation. One of the new directions is to use supported lipid bilayer (SLB) platforms to separate the membrane species while they are protected in their native environment. Here we used a type of crosslinkable diacetylene phospholipids, diynePC (1,2-bis(10,12-tricosadiynoyl)-sn-glycero-3-phosphocholine), as a packed material to create a 'two-dimensional (2D) packed bed' in a SLB platform. After the diynePC SLB is exposed to UV light, some of the diynePC lipids in the SLB can crosslink and the non-crosslinked monomer lipids can be washed away, leaving a 2D porous solid matrix. We incorporated the lipid vesicle deposition method with a microfluidic device to pattern the location of the packed-bed region and the feed region with species to be separated in a SLB platform. Our atomic force microscopy result shows that the nano-scaled structure density of the '2D packed bed' can be tuned by the UV dose applied to the diynePC membrane. When the model membrane biomolecules were forced to transport through the packed-bed region, their concentration front velocities were found to decrease linearly with the UV dose, indicating the successful creation of packed obstacles in these 2D lipid membrane separation platforms.
authors.affiliation: Department of Chemical Engineering, National Taiwan University, No. 1, Section 4, Roosevelt Road, Taipei 10617, Taiwan.
authors.forename: Shu-Kai
authors.initials: SK
authors.lastname: Hu
authors.affiliation: R&D Center for Membrane Technology and Department of Chemical Engineering, Chung Yuan Christian University, Jhong-Li, Taoyuan 320, Taiwan.
authors.forename: Sheng-Wen
authors.initials: SW
authors.lastname: Hsiao
authors.affiliation: Department of Chemical Engineering, National Taiwan University, No. 1, Section 4, Roosevelt Road, Taipei 10617, Taiwan.
authors.forename: Hsun-Yen
authors.initials: HY
authors.lastname: Mao
authors.affiliation: Department of Chemical Engineering, National Taiwan University, No. 1, Section 4, Roosevelt Road, Taipei 10617, Taiwan.
authors.forename: Ya-Ming
authors.initials: YM
authors.lastname: Chen
authors.affiliation: R&D Center for Membrane Technology and Department of Chemical Engineering, Chung Yuan Christian University, Jhong-Li, Taoyuan 320, Taiwan.
authors.forename: Yung
authors.initials: Y
authors.lastname: Chang
authors.affiliation: Department of Chemical Engineering, National Taiwan University, No. 1, Section 4, Roosevelt Road, Taipei 10617, Taiwan.
authors.forename: Ling
authors.initials: L
authors.lastname: Chao
keywords: 10.01; 10.13; 20.11; 2D packed bed; crosslinking; diacetylene phospholipids; separation; supported lipid bilayers