title: Polymorphism of growth hormone receptor (GHR) gene in Nilagiri sheep.
abstract: The allelic variation in the regulatory sequence of growth hormone receptor (GHR) gene influences the growth traits of sheep. A study was carried out to find out the polymorphisms associated with exon 10 of GHR gene and its association with growth traits of Nilagiri sheep. The blood samples were collected from Nilagiri sheep (n = 103) reared at Sheep Breeding Research Station, Sandynallah, Tamil Nadu, India. DNA was isolated using the phenol-chloroform extraction procedure and eight samples having amplified product of part of exon 10 (895 bp) sequenced. The results indicated transitions of nucleotide G>A at loci G177624A and G177878A. The genotyping frequencies estimated using the tetra-primer amplification refractory mutation system-PCR for GG, GA and AA were 0.262, 0.544 and 0.194, and 0.349, 0.505 and 0.146, respectively. The estimated allele frequencies of G and A nucleotides were 0.5340 and 0.4660, and 0.6015 and 0.3985, respectively, at loci G177624A and G177878A. The effects of both the mutations on growth-related traits viz., birth, weaning (3 months) 6, 9 and 12 months weight in Nilagiri sheep were found to be non-significant. This can be a novel approach to assess growth of sheep using the mutation in GHR gene. Thus, this approach can be useful for further investigation as a molecular marker associated with genetic improvement.
authors.affiliation: Department of Animal Genetics and Breeding, Madras Veterinary College, Chennai, 600007, India. dramiyavet@gmail.com.
authors.forename: Amiya Ranjan
authors.initials: AR
authors.lastname: Sahu
authors.affiliation: Department of Animal Genetics and Breeding, Veterinary College and Research Institute, Orathanadu, Thanjavur, 614625, India.
authors.forename: V
authors.initials: V
authors.lastname: Jeichitra
authors.affiliation: Directorate of Research, TANUVAS, Madhavaram Milk Colony, Chennai, 600051, India.
authors.forename: R
authors.initials: R
authors.lastname: Rajendran
authors.affiliation: Department of Animal Biotechnology, Madras Veterinary College, Chennai, 600007, India.
authors.forename: A
authors.initials: A
authors.lastname: Raja
mesh_terms: D000818:Animals; D001483:Base Sequence; D005091:Exons; D005260:Female; D005787:Gene Frequency; D007194:India; D008297:Male; D016133:Polymerase Chain Reaction / Q000662:veterinary; D020641:Polymorphism, Single Nucleotide; D011986:Receptors, Somatotropin / Q000235:genetics* / Q000378:metabolism; D034561:Sheep, Domestic / Q000235:genetics* / Q000254:growth & development
chemical_list: D011986:Receptors, Somatotropin
keywords: Growth hormone receptor; Nilagiri sheep; SNP; Tetra-primer ARMS-PCR