title: Profiling of volatile organic compounds released from individual intact juvenile and mature citrus leaves.
abstract: Plants release volatiles to communicate with each other and to attract or repel insects. The methods used to collect volatiles are varied. Here, we describe a simple solvent-less, solid phase microextraction-based method to collect the volatiles released from intact citrus leaves. We were able to collect up to 39 volatiles from both juvenile and mature leaves. Our results indicated that juvenile leaves produced both monoterpenes and sesquiterpenes, and while mature leaves continued to produce a variety of monoterpenes, their release of sesquiterpenes decreased dramatically. The finding that juvenile leaves emitted higher levels of sesquiterpenes while mature leaves released mostly monoterpenes suggests that younger leaves of plants may be involved in a more complex chemical communication system.
authors.affiliation: Department of Plant Pathology, Citrus Research and Education Center, University of Florida, 700 Experiment Station Rd., Lake Alfred, FL 33850, United States. Electronic address: nabilkilliny@ufl.edu.
authors.forename: Nabil
authors.initials: N
authors.lastname: Killiny
authors.affiliation: Department of Plant Pathology, Citrus Research and Education Center, University of Florida, 700 Experiment Station Rd., Lake Alfred, FL 33850, United States.
authors.forename: Shelley E
authors.initials: SE
authors.lastname: Jones
mesh_terms: D002957:Citrus / Q000737:chemistry* / Q000502:physiology; D039821:Monoterpenes / Q000737:chemistry* / Q000378:metabolism; D009928:Organ Specificity; D018515:Plant Leaves / Q000737:chemistry / Q000502:physiology; D012717:Sesquiterpenes / Q000737:chemistry* / Q000378:metabolism; D013997:Time Factors; D055549:Volatile Organic Compounds / Q000737:chemistry* / Q000378:metabolism
chemical_list: D039821:Monoterpenes; D012717:Sesquiterpenes; D055549:Volatile Organic Compounds
keywords: Citrus; GC–MS; Monoterpenes; Organic volatile compounds; SPME; Sesquiterpenes