title: Ultra high performance liquid chromatography-electrospray ionization-tandem mass spectrometry and pharmacokinetic analysis of justicidin B and 6'-hydroxy justicidin C in rats.
abstract: Arylnaphthalene lignans have attracted considerable interest with the discovery of their antineoplastic activities. Two such compounds are justicidin B and 6'-hydroxy justicidin C, both of which have been isolated from the herb Justicia procumbens. We sought to develop and validate a sensitive and accurate, ultra high performance liquid chromatography with electrospray ionization tandem mass spectrometry method for the structural determination and pharmacokinetics of justicidin B and 6'-hydroxy justicidin C. Chromatographic separation was achieved on an Agilent 300SB-C18column using water (0.5% formic acid, 10 mM NH4COOH) methanol as the mobile phase. The plasma samples obtained after oral administration of the active extract of Justicia procumbens were successfully analyzed with our novel method, thereby demonstrating its sound applicability and reliability. The lower limit of quantification for justicidin B and 6'-hydroxy justicidin C was 0.50 and 1.00 ng/mL in 50 μL rat plasma, respectively. The elimination half-life and clearance of justicidin B was estimated to be 1.27 ± 0.61 h and 5.40 ± 0.22 L/h/kg while that of 6'-hydroxy justicidin C was 2.07 ± 0.70 h and 11.84 ± 1.06 L/h/kg. This newly developed and validated method was successfully applied to the quantification and pharmacokinetic study of justicidin B and 6'-hydroxy justicidin C in rats.
authors.affiliation: Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, P.R. China.
authors.forename: Jiaoyang
authors.initials: J
authors.lastname: Luo
authors.affiliation: Chengdu University, School of pharmacy and bioengineering, Chengdu, P.R. China.
authors.forename: Yichen
authors.initials: Y
authors.lastname: Hu
authors.affiliation: Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, P.R. China.|Southwest Jiaotong University, School of Life Science and Engineering, Chengdu, P.R. China.
authors.forename: Jia'an
authors.initials: J
authors.lastname: Qin
authors.affiliation: Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, P.R. China.
authors.forename: Meihua
authors.initials: M
authors.lastname: Yang
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid; D004148:Dioxolanes / Q000032:analysis* / Q000097:blood / Q000493:pharmacokinetics*; D006207:Half-Life; D017705:Lignans / Q000032:analysis* / Q000097:blood / Q000493:pharmacokinetics*; D057230:Limit of Detection; D051381:Rats; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results; D021241:Spectrometry, Mass, Electrospray Ionization; D053719:Tandem Mass Spectrometry
chemical_list: D004148:Dioxolanes; D017705:Lignans; C049719:justicidins
keywords: 6′-hydroxy justicidin C; Justicia procumbens; justicidin B; pharmacokinetics; traditional Chinese medicine