title: Polymerizing Pyrrole Coated Poly (l-lactic acid-co-ε-caprolactone) (PLCL) Conductive Nanofibrous Conduit Combined with Electric Stimulation for Long-Range Peripheral Nerve Regeneration.
abstract: Electrospinning and electric stimulation (ES) are both promising methods to support neuron adhesion and guide extension of neurons for nerve regeneration. Concurrently, all studies focus on either electrospinning for conduits material or ESin vitrostudy to accelerate nerve regeneration; few work on the combined use of these two strategies or ESin vivostudy. Therefore, this study aimed to investigate the abilities of direct current ES through electrospinning conductive polymer composites composed of polypyrrole and Poly (l-lactic acid-co-ε-caprolactone) (PPY/PLCL) in peripheral nerve regeneration. PPY/PLCL composite conduits were synthesized by polymerizing pyrrole coated electrospun PLCL scaffolds. Morphologies and chemical compositions were characterized by scanning electron microscope and attenuated total reflection fourier transform infrared (ATR-FTIR) microscope. Rat pheochromocytoma 12 (PC12) cells and dorsal root ganglia (DRG) cells cultured on PPY/PLCL scaffolds were stimulated with 100 mV/cm for 4 h per day. The median neurite length and cell viability were measured in PC-12 cells. The levels of brain-derived neurotrophic factor (BDNF), glial cell derived neurotrophic factor (GDNF) and neurotrophin-3 (NT-3) were analyzed in DRG cells. In rats, 15 mm gaps of sciatic nerves were bridged using an autograft, non-stimulated PPY/PLCL conduit and PPY/PLCL conduit stimulated with 100 mV potential, respectively. A 100 mV potential direct current ES was applied for 1 h per day at 1, 3, 5 and 7 days post-implantation. The PPY/PLCL conduits with ES showed a similar performance compared with the autograft group, and significantly better than the non-stimulated PPY/PLCL conduit group. These promising results show that the PPY/PLCL conductive conduits' combined use with ES has great potential for peripheral nerve regeneration.
authors.affiliation: Shanghai Jiao Tong University Affiliated Sixth People's HospitalShanghai, China; Shanghai Sixth People's Hospital East Campus, Shanghai University of Medicine and HealthShanghai, China.
authors.forename: Jialin
authors.initials: J
authors.lastname: Song
authors.affiliation: College of Chemistry, Chemical Engineering and Biotechnology, Donghua University Shanghai, China.
authors.forename: Binbin
authors.initials: B
authors.lastname: Sun
authors.affiliation: Shanghai Jiao Tong University Affiliated Sixth People's Hospital Shanghai, China.
authors.forename: Shen
authors.initials: S
authors.lastname: Liu
authors.affiliation: Shanghai Jiao Tong University Affiliated Sixth People's Hospital Shanghai, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Chen
authors.affiliation: Changhai Hospital of Second Military Medical University Shanghai, China.
authors.forename: Yuanzheng
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Shanghai Jiao Tong University Affiliated Sixth People's Hospital Shanghai, China.
authors.forename: Chunyang
authors.initials: C
authors.lastname: Wang
authors.affiliation: College of Chemistry, Chemical Engineering and Biotechnology, Donghua University Shanghai, China.
authors.forename: Xiumei
authors.initials: X
authors.lastname: Mo
authors.affiliation: School of Pharmacy, Shanghai Jiao Tong University Shanghai, China.
authors.forename: Junyi
authors.initials: J
authors.lastname: Che
authors.affiliation: Shanghai Jiao Tong University Affiliated Sixth People's HospitalShanghai, China; Shanghai Sixth People's Hospital East Campus, Shanghai University of Medicine and HealthShanghai, China.
authors.forename: Yuanming
authors.initials: Y
authors.lastname: Ouyang
authors.affiliation: School of Pharmacy, Shanghai Jiao Tong University Shanghai, China.
authors.forename: Weien
authors.initials: W
authors.lastname: Yuan
authors.affiliation: Shanghai Jiao Tong University Affiliated Sixth People's Hospital Shanghai, China.
authors.forename: Cunyi
authors.initials: C
authors.lastname: Fan
keywords: conductive polymer; electric stimulation; nanofibers; nerve regeneration; peripheral nerve injury