title: Paper-based tuberculosis diagnostic devices with colorimetric gold nanoparticles.
abstract: A colorimetric sensing strategy employing gold nanoparticles and a paper assay platform has been developed for tuberculosis diagnosis. Unmodified gold nanoparticles and single-stranded detection oligonucleotides are used to achieve rapid diagnosis without complicated and time-consuming thiolated or other surface-modified probe preparation processes. To eliminate the use of sophisticated equipment for data analysis, the color variance for multiple detection results was simultaneously collected and concentrated on cellulose paper with the data readout transmitted for cloud computing via a smartphone. The results show that the 2.6 nM tuberculosis mycobacterium target sequences extracted from patients can easily be detected, and the turnaround time after the human DNA is extracted from clinical samples was approximately 1 h.
authors.affiliation: Department of Orthopaedic Surgery, Chang Gung Memorial Hospital, Taipei 105, Taiwan; Graduate Institute of Clinical Medical Sciences, Chang Gung University, Taoyuan 333, Taiwan.
authors.forename: Tsung-Ting
authors.initials: TT
authors.lastname: Tsai
authors.affiliation: Graduate Institute of Biomedical Engineering, National Chung Hsing University, Taichung 402, Taiwan.
authors.forename: Shu-Wei
authors.initials: SW
authors.lastname: Shen
authors.affiliation: Institute of Nanoengineering and Microsystems, National Tsing Hua University, Hsinchu 300, Taiwan.
authors.forename: Chao-Min
authors.initials: CM
authors.lastname: Cheng
authors.affiliation: Graduate Institute of Biomedical Engineering, National Chung Hsing University, Taichung 402, Taiwan.
authors.forename: Chien-Fu
authors.initials: CF
authors.lastname: Chen
keywords: Tuberculosis diagnostics; colorimetric assay; microfluidic paper-based analytical devices