title: -2518 A/GMCP-1but not -403 G/ARANTESgene polymorphism is associated with enhanced risk of basal cell carcinoma.
abstract: BACKGROUND
  Polymorphic variants ofMCP-1andRANTESgenes and their protein serum levels have been implicated in the increased risk and severity of several malignancies. However, the subject has not been explored in basal cell carcinoma (BCC) patients so far.
  
  
  OBJECTIVE
  To investigate the association between monocyte chemoattractant protein 1 (MCP-1) (-2518 A/G) andRANTES(-403 G/A) polymorphism and risk and clinical course of BCC.
  
  
  METHODS
  The study group consisted of 150 unrelated patients with BCC and 140 healthy, unrelated, age- and sex-matched volunteers. The polymorphisms were analysed using the amplification refractory mutation system polymerase chain reaction method (ARMS-PCR) and single specific primer-polymerase chain reaction (SSP-PCR). Serum cytokine levels were measured with ELISA.
  
  
  RESULTS
  The presence of theMCP-1-2518 GG genotype was statistically more frequent in BCC patients and it increased the risk of BCC (OR = 2.63,p= 0.003). Genotype -330 GG was statistically more common in patients with less advanced tumours (OR = 2.8,p= 0.017). Monocyte chemoattractant protein 1 serum level was statistically higher with GG genotype. In the BCC group MCP-1 serum levels were decreased. Neither polymorphic variants ofRANTESnor the chemokine serum concentration differed significantly between the study groups.
  
  
  CONCLUSIONS
  These findings suggest that -2518 A/GMCP-1polymorphism may be involved in BCC pathogenesis.
authors.affiliation: Department of Dermatology, Venereology and Allergology, Medical University of Gdansk, Gdansk, Poland.
authors.forename: Michał
authors.initials: M
authors.lastname: Sobjanek
authors.affiliation: Department of Dermatology, Venereology and Allergology, Medical University of Gdansk, Gdansk, Poland.
authors.forename: Monika
authors.initials: M
authors.lastname: Zabłotna
authors.affiliation: Department of Dermatology, Venereology and Allergology, Medical University of Gdansk, Gdansk, Poland.
authors.forename: Aneta
authors.initials: A
authors.lastname: Szczerkowska-Dobosz
authors.affiliation: Department of Pathology and Experimental Rheumatology, Medical University of Gdansk, Gdansk, Poland.
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Ruckemann-Dziurdzińska
authors.affiliation: Department of Dermatology, Venereology and Allergology, Medical University of Gdansk, Gdansk, Poland.
authors.forename: Malgorzata
authors.initials: M
authors.lastname: Sokolowska-Wojdylo
authors.affiliation: Department of Dermatology, Venereology and Allergology, Medical University of Gdansk, Gdansk, Poland.
authors.forename: Roman
authors.initials: R
authors.lastname: Nowicki
keywords: BCC; CCL2; CCL5; RANTES; basal cell carcinoma; gene polymorphism; monocyte chemo-attractant protein 1; monocyte chemoattractant protein 1; regulated upon activation normal T-cell expressed and secreted