title: Polymorphism in CRHR1 gene affects the IL-1β levels in suicidal attempters.
abstract: Approximately one million people commit suicide every year, being suicide attempts and ideation even more common. Changes in stress response and activation of the immune system have been associated with suicide risk. Here we investigated the interaction between immune system and HPA axis alterations in the suicide risk, looking for the influence of rs110402 CRHR1 SNP in the IL-1β levels according to suicide ideation and attempt. This study evaluated 171 subjects of which 15 had suicidal ideation, 20 had suicide attempt and 136 were controls. Genotyping was performed by real-time PCR and IL-1β levels were measured by ELISA. Our data showed that for each point increase in IL-1β levels the risk of suicide attempt increased 5% [relative risk = 1.05 (95% CI: 1.0-1.10)]. After sample stratification by rs110402 SNP genotypes, we observed that in subjects carrying the A allele the risk raised to 15% [relative risk = 1.15 (95% CI: 1.03-1.28)], suggesting an apparent effect modification. Thus, this study showed that alterations in CRHR1 gene were associated with higher levels of IL-1β, and increased risk for suicide, reinforcing the importance of multifactorial interactions of biological markers for psychiatric disorders.
authors.affiliation: Programa de Pós-Graduação em Saúde e Comportamento, Universidade Católica de Pelotas, Brazil.
authors.forename: Clarissa R
authors.initials: CR
authors.lastname: Bastos
authors.affiliation: Biologia Celular e Molecular, Pontifícia Universidade Católica do Rio Grande do Sul, Brazil.
authors.forename: Marta
authors.initials: M
authors.lastname: Gazal
authors.affiliation: Programa de Pós-Graduação em Saúde e Comportamento, Universidade Católica de Pelotas, Brazil.
authors.forename: Luciana de A
authors.initials: LA
authors.lastname: Quevedo
authors.affiliation: Programa de Pós-Graduação em Saúde e Comportamento, Universidade Católica de Pelotas, Brazil.
authors.forename: Joice Luisa
authors.initials: JL
authors.lastname: Costa
authors.affiliation: Programa de Pós-Graduação em Saúde e Comportamento, Universidade Católica de Pelotas, Brazil.
authors.forename: Carolina D
authors.initials: CD
authors.lastname: Wiener
authors.affiliation: Programa de Pós-Graduação em Saúde e Comportamento, Universidade Católica de Pelotas, Brazil.
authors.forename: Karen
authors.initials: K
authors.lastname: Jansen
authors.affiliation: Programa de Pós-Graduação em Epidemiologia, Universidade Federal de Pelotas, Brazil.
authors.forename: Christian Loret
authors.initials: CL
authors.lastname: de Mola
authors.affiliation: Programa de Pós-Graduação em Saúde e Comportamento, Universidade Católica de Pelotas, Brazil.
authors.forename: Jean P
authors.initials: JP
authors.lastname: Oses
authors.affiliation: Programa de Pós-Graduação em Saúde e Comportamento, Universidade Católica de Pelotas, Brazil.
authors.forename: Luciano D M
authors.initials: LDM
authors.lastname: Souza
authors.affiliation: Departamento de Bioquímica, Universidade Federal do Rio Grande do Sul, Porto Alegre, Rio Grande do Sul, Brazil.
authors.forename: Luiz Valmor
authors.initials: LV
authors.lastname: Portela
authors.affiliation: Programa de Pós-Graduação em Saúde e Comportamento, Universidade Católica de Pelotas, Brazil.
authors.forename: Ricardo T
authors.initials: RT
authors.lastname: Pinheiro
authors.affiliation: Programa de Pós-Graduação em Saúde e Comportamento, Universidade Católica de Pelotas, Brazil.
authors.forename: Ricardo A
authors.initials: RA
authors.lastname: da Silva
authors.affiliation: Biologia Celular e Molecular, Pontifícia Universidade Católica do Rio Grande do Sul, Brazil.
authors.forename: Diogo R
authors.initials: DR
authors.lastname: Lara
authors.affiliation: Programa de Pós-Graduação em Saúde e Comportamento, Universidade Católica de Pelotas, Brazil. Electronic address: ghisleni.g@gmail.com.
authors.forename: Gabriele
authors.initials: G
authors.lastname: Ghisleni
mesh_terms: D000293:Adolescent; D000328:Adult; D015415:Biomarkers / Q000097:blood; D003430:Cross-Sectional Studies; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D060005:Genotyping Techniques; D006801:Humans; D053583:Interleukin-1beta / Q000097:blood*; D016015:Logistic Models; D008297:Male; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide; D018019:Receptors, Corticotropin-Releasing Hormone / Q000235:genetics*; D059020:Suicidal Ideation; D013406:Suicide, Attempted; D055815:Young Adult; D000099052:CRF Receptor, Type 1
chemical_list: D015415:Biomarkers; C508585:IL1B protein, human; D053583:Interleukin-1beta; D018019:Receptors, Corticotropin-Releasing Hormone; D000099052:CRF Receptor, Type 1
keywords: CRHR1; HPA axis; Inflammation; Interleukin-1β; Polymorphism; Suicide risk