title: Molecular cloning and nutrient regulation analysis of long chain acyl-CoA synthetase 1 gene in grass carp, Ctenopharyngodon idella L.
abstract: Long chain acyl-CoA synthetase 1 (ACSL1), a key regulatory enzyme of fatty acid metabolism, catalyzes the conversion of long-chain fatty acids to acyl-coenzyme A. The full-length cDNAs of ACSL1a and ACSL1b were cloned from the liver of a grass carp. Both cDNAs contained a 2094bp open reading frame encoding 697 amino acids. Amino acid sequence alignment showed that ACSL1a shared 73.5% sequence identity with ACSL1b. Each of the two ACSL1s proteins had a transmembrane domain, a P-loop domain, and L-, A-, and G-motifs, which were relatively conserved in comparison to other vertebrates. Relative expression profile of ACSL1 mRNAs in different tissues indicated that ACSL1a is highly expressed in heart, mesenteric adipose, and brain tissues, whereas ACSL1b is highly expressed in heart, white muscle, foregut, and liver tissues. Nutrient regulation research showed that the expression levels of ACSL1a and ACSL1b were significantly down-regulated when 3, 6, and 9% fish oil were added in diet of grass carp as compared to the control group. However, no significant difference in the levels of ACSL1 mRNA was observed between the experimental groups. This study demonstrated the relationship between ACSL1a and ACSL1b genes in grass carp and laid a foundation for further research on ACSL family members in other species.
authors.affiliation: Jiangsu Key Laboratory of Marine Biotechnology, Huaihai Institute of Technology, Lianyungang 222005, China; Co-Innovation Center of Jiangsu Marine Bio-industry Technology, Lianyungang 222005, China. Electronic address: CHL3139@163.com.
authors.forename: Han-Liang
authors.initials: HL
authors.lastname: Cheng
authors.affiliation: Jiangsu Key Laboratory of Marine Biotechnology, Huaihai Institute of Technology, Lianyungang 222005, China; Beijing Institute of Life Science, Chinese Academy of Science, Beijing 100101, China. Electronic address: shengjichenshuai@163.com.
authors.forename: Shuai
authors.initials: S
authors.lastname: Chen
authors.affiliation: Jiangsu Key Laboratory of Marine Biotechnology, Huaihai Institute of Technology, Lianyungang 222005, China; Co-Innovation Center of Jiangsu Marine Bio-industry Technology, Lianyungang 222005, China.
authors.forename: Jian-He
authors.initials: JH
authors.lastname: Xu
authors.affiliation: Jiangsu Key Laboratory of Marine Biotechnology, Huaihai Institute of Technology, Lianyungang 222005, China.
authors.forename: Le-Fei
authors.initials: LF
authors.lastname: Yi
authors.affiliation: Jiangsu Key Laboratory of Marine Biotechnology, Huaihai Institute of Technology, Lianyungang 222005, China.
authors.forename: Yong-Xing
authors.initials: YX
authors.lastname: Peng
authors.affiliation: Zhejiang Institute of Freshwater Fisheries, Huzhou 313001, China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Pan
authors.affiliation: Jiangsu Key Laboratory of Marine Biotechnology, Huaihai Institute of Technology, Lianyungang 222005, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Shen
authors.affiliation: Jiangsu Key Laboratory of Marine Biotechnology, Huaihai Institute of Technology, Lianyungang 222005, China.
authors.forename: Zhi-Guo
authors.initials: ZG
authors.lastname: Dong
authors.affiliation: Jiangsu Key Laboratory of Marine Biotechnology, Huaihai Institute of Technology, Lianyungang 222005, China.
authors.forename: Xia-Qing
authors.initials: XQ
authors.lastname: Zhang
authors.affiliation: Jiangsu Key Laboratory of Marine Biotechnology, Huaihai Institute of Technology, Lianyungang 222005, China.
authors.forename: Wen-Xiang
authors.initials: WX
authors.lastname: Wang
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002347:Carps / Q000235:genetics* / Q000378:metabolism*; D003001:Cloning, Molecular; D003066:Coenzyme A Ligases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D004032:Diet; D015971:Gene Expression Regulation, Enzymologic; D009928:Organ Specificity; D010802:Phylogeny; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism
chemical_list: D012333:RNA, Messenger; D003066:Coenzyme A Ligases
keywords: Grass carp; Long chain acyl-CoA synthetase 1; Molecular cloning; Nutrient regulation