title: Bovine serum albumin coated nanoparticles for in vitro activated fluorescence.
abstract: A fluorophore modified nanoparticle was developed that can only fluoresce when a specific environmental parameter interacts with the system. The model system consisted of an azide modified bovine serum albumin (azBSA) that had been covalently attached to an alkyne modified silicon phthalocyanine (alSiPc) derivative through a copper catalyzed azide/alkyne Huisgen cycloaddition (click reaction). The azBSA/alSiPc assembly was then clicked to a ca. 67 nm poly(propargyl acrylate) (PA) nanoparticle (PA/azBSA/alSiPc). The resulting particles did not exhibit any florescence when the alSiPc was excited. Incubating the particles at 37 °C for 30 min with a proteolytic enzyme (trypsin) degraded the linking BSA and resulted in the appearance of florescence that was attributed to a "free" silicon phthalocyanine. The PA/azBSA/alSiPc particles were incubated with human non-small cell lung cancer cells (A549) and the florescence of the initially quenched particles was achieved with cellular uptake.
authors.affiliation: Clemson University, Department of Materials Science and Engineering, Advanced Materials Research Laboratory 226, Anderson, SC 29625, USA. foulger@clemson.edu.
authors.forename: Mary K
authors.initials: MK
authors.lastname: Burdette
authors.affiliation: Clemson University, Department of Materials Science and Engineering, Advanced Materials Research Laboratory 226, Anderson, SC 29625, USA. foulger@clemson.edu.
authors.forename: Ragini
authors.initials: R
authors.lastname: Jenkins
authors.affiliation: Clemson University, Department of Materials Science and Engineering, Advanced Materials Research Laboratory 226, Anderson, SC 29625, USA. foulger@clemson.edu and Center for Optical Materials Science and Engineering Technologies (COMSET), Advanced Materials Research Laboratory 226, Anderson, SC, USA.
authors.forename: Yuriy
authors.initials: Y
authors.lastname: Bandera
authors.affiliation: Clemson Light Imaging Facility, Clemson University, Clemson, SC 29634, USA.
authors.forename: Rhonda R
authors.initials: RR
authors.lastname: Powell
authors.affiliation: Clemson Light Imaging Facility, Clemson University, Clemson, SC 29634, USA.
authors.forename: Terri F
authors.initials: TF
authors.lastname: Bruce
authors.affiliation: Department of Biological Sciences, Clemson University, Clemson, SC 29634-0971, USA.
authors.forename: Xi
authors.initials: X
authors.lastname: Yang
authors.affiliation: Department of Biological Sciences, Clemson University, Clemson, SC 29634-0971, USA.
authors.forename: Yanzhang
authors.initials: Y
authors.lastname: Wei
authors.affiliation: Clemson University, Department of Materials Science and Engineering, Advanced Materials Research Laboratory 226, Anderson, SC 29625, USA. foulger@clemson.edu and Center for Optical Materials Science and Engineering Technologies (COMSET), Advanced Materials Research Laboratory 226, Anderson, SC, USA and Department of Bioengineering, Clemson University, Clemson, SC 29634, USA.
authors.forename: Stephen H
authors.initials: SH
authors.lastname: Foulger