title: A microarray whole-genome gene expression dataset in a rat model of inflammatory corneal angiogenesis.
abstract: In angiogenesis with concurrent inflammation, many pathways are activated, some linked to VEGF and others largely VEGF-independent. Pathways involving inflammatory mediators, chemokines, and micro-RNAs may play important roles in maintaining a pro-angiogenic environment or mediating angiogenic regression. Here, we describe a gene expression dataset to facilitate exploration of pro-angiogenic, pro-inflammatory, and remodelling/normalization-associated genes during both an active capillary sprouting phase, and in the restoration of an avascular phenotype. The dataset was generated by microarray analysis of the whole transcriptome in a rat model of suture-induced inflammatory corneal neovascularisation. Regions of active capillary sprout growth or regression in the cornea were harvested and total RNA extracted from four biological replicates per group. High quality RNA was obtained for gene expression analysis using microarrays. Fold change of selected genes was validated by qPCR, and protein expression was evaluated by immunohistochemistry. We provide a gene expression dataset that may be re-used to investigate corneal neovascularisation, and may also have implications in other contexts of inflammation-mediated angiogenesis.
authors.affiliation: Department of Ophthalmology, Institute for Clinical and Experimental Medicine, Faculty of Health Sciences, Linkoping University, Linköping 58183, Sweden.
authors.forename: Anthony
authors.initials: A
authors.lastname: Mukwaya
authors.affiliation: National Bioinformatics Infrastructure Sweden (NBIS) Science for Life Laboratory, Department of Biochemistry and Biophysics, Stockholm University, Stockholm S-10691, Sweden.
authors.forename: Jessica M
authors.initials: JM
authors.lastname: Lindvall
authors.affiliation: Department of Ophthalmology, Institute for Clinical and Experimental Medicine, Faculty of Health Sciences, Linkoping University, Linköping 58183, Sweden.
authors.forename: Maria
authors.initials: M
authors.lastname: Xeroudaki
authors.affiliation: Department of Ophthalmology, Institute for Clinical and Experimental Medicine, Faculty of Health Sciences, Linkoping University, Linköping 58183, Sweden.
authors.forename: Beatrice
authors.initials: B
authors.lastname: Peebo
authors.affiliation: Department of Medical and Health Sciences, Division of Cardiovascular Medicine, Linköping University, Linköping 581 83, Sweden.
authors.forename: Zaheer
authors.initials: Z
authors.lastname: Ali
authors.affiliation: Department of Ophthalmology, Institute for Clinical and Experimental Medicine, Faculty of Health Sciences, Linkoping University, Linköping 58183, Sweden.
authors.forename: Anton
authors.initials: A
authors.lastname: Lennikov
authors.affiliation: Department of Medical and Health Sciences, Division of Cardiovascular Medicine, Linköping University, Linköping 581 83, Sweden.|Department of Microbiology, Tumor and Cell biology, the Karolinska Institute, Stockholm 17177, Sweden.
authors.forename: Lasse Dahl Ejby
authors.initials: LD
authors.lastname: Jensen
authors.affiliation: Department of Ophthalmology, Institute for Clinical and Experimental Medicine, Faculty of Health Sciences, Linkoping University, Linköping 58183, Sweden.
authors.forename: Neil
authors.identifier: 0000-0003-1079-4361
authors.initials: N
authors.lastname: Lagali
mesh_terms: D000818:Animals; D016510:Corneal Neovascularization / Q000235:genetics*; D004195:Disease Models, Animal; D015870:Gene Expression; D016678:Genome; D046228:Microarray Analysis; D009389:Neovascularization, Pathologic; D051381:Rats