title: miR-508-5p acts as an anti-oncogene by targeting MESDC1 in hepatocellular carcinoma.
abstract: Hepatocellular carcinoma (HCC) is the third leading cause of cancer associated mortality. Accumulating evidence has shown that microRNAs (miRNAs) act as critical factors for tumor recurrence and metastasis. MiR-508-5p has been reported as a down-regulated miRNA in the primary gastric cancer tissues. However, the role of miR-508-5p on HCC has not been well elucidated. In this study, we observed that miR-508-5p was downregulated in HCC tissues when compared to the non-tumorous tissues. We then demonstrated that overexpression of miR-508-5p attenuated HepG2 cells proliferation and invasion and induced cell apoptosis in vitro. Furthermore, our further investigations revealed that mesoderm development candidate 1 (MESDC1) is a potential target of miR-508-5p, as well as miR-508-5p overexpression downregulated MESDC1 expression. Overexpression of MESDC1 promoted HepG2 cells migration, invasion and proliferation in vitro. In addition, miR-508-5p markedly suppressed the tumor growth in xenograft model, while MESDC1 promoted the tumor growth in xenograft model. This study provides new insight into molecular mechanisms that miR-508-5p acts as a tumor suppressor by targeting MESDC1 in HCC progression.
authors.forename: S G
authors.initials: SG
authors.lastname: Wu
authors.forename: Y J
authors.initials: YJ
authors.lastname: Huang
authors.forename: B
authors.initials: B
authors.lastname: Bao
authors.forename: L M
authors.initials: LM
authors.lastname: Wu
authors.forename: J
authors.initials: J
authors.lastname: Dong
authors.forename: X H
authors.initials: XH
authors.lastname: Liu
authors.forename: Z H
authors.initials: ZH
authors.lastname: Li
authors.forename: X Y
authors.initials: XY
authors.lastname: Wang
authors.forename: L
authors.initials: L
authors.lastname: Wang
authors.forename: B J
authors.initials: BJ
authors.lastname: Chen
authors.forename: W
authors.initials: W
authors.lastname: Chen
mesh_terms: D000818:Animals; D006528:Carcinoma, Hepatocellular / Q000235:genetics*; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D015972:Gene Expression Regulation, Neoplastic; D016147:Genes, Tumor Suppressor; D056945:Hep G2 Cells; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics*; D035683:MicroRNAs / Q000235:genetics*; D018832:Molecular Chaperones / Q000378:metabolism*; D009364:Neoplasm Recurrence, Local; D023041:Xenograft Model Antitumor Assays
chemical_list: C571981:MIRN508 microRNA, human; D035683:MicroRNAs; D018832:Molecular Chaperones; C574664:TLNRD1 protein, human
keywords: MESDC1 hepatocellular carcinoma.; miR-508-5p