title: Simultaneous determination of anemoside B4, phellodendrine, berberine, palmatine, obakunone, esculin, esculetin in rat plasma by UPLC-ESI-MS/MS and its application to a comparative pharmacokinetic study in normal and ulcerative colitis rats.
abstract: A sensitive and rapid ultra-performance liquid chromatography-electrospray ionization-mass spectrometry (UPLC-ESI-MS/MS) method was developed for the simultaneous analysis of anemoside B4, phellodendrine, berberine, palmatine, obakunone, esculin, esculetin, toosendanin (IS1of anemoside B4), tetrahydropalmatine (IS2of phellodendrine, berberine, palmatine and obakunone) and scopoletin (IS3of esculin and esculetin) and to compare the pharmacokinetics of these active ingredients in normal and ulcerative colitis rats. After methanol deproteinization, solvents were evaporated at 40°C under a gentle stream of nitrogen. Chromatography was performed using a C18 column with a gradient elution of 0.1% aqueous formic acid and acetonitrile at 0.4ml/min. Detection and measurement were performed on a 4000 QTRAP UPLC-MS/MS system from AB Sciex in the multiple reaction monitoring (MRM) mode. Phellodendrine, berberine, palmatine, obakunone, esculin, esculetin, tetrahydropalmatine (IS2) and scopoletin (IS3) were monitored under positive ionization conditions. Anemoside B4, and toosendanin (IS1) were monitored under negative ionization conditions. The optimized mass transition ion-pairs (m/z) were 1221.1/750.7 for anemoside B4, 343.2/193.2 for phellodendrine, 337.1/321.0 for berberine, 353.0/336.9 for palmatine, 455.1/161.1 for obakunone, 341.2/179.2 for esculin, 179.1/123.0 for esculetin, 573.4/531.4 for toosendanin (IS1), 356.2/192.2 for tetrahydropalmatine (IS2) and 193.0/133.1 for scopoletin (IS3).
authors.affiliation: School of Pharmacy, Heilongjiang University of Traditional Chinese Medicine, Harbin 150040, Heilongjiang, China. Electronic address: lianrong_790717@sina.com.
authors.forename: Lianrong
authors.initials: L
authors.lastname: Yang
authors.affiliation: School of Pharmacy, Heilongjiang University of Traditional Chinese Medicine, Harbin 150040, Heilongjiang, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Meng
authors.affiliation: School of Pharmacy, Heilongjiang University of Traditional Chinese Medicine, Harbin 150040, Heilongjiang, China.
authors.forename: Xiaojin
authors.initials: X
authors.lastname: Yu
authors.affiliation: School of Pharmacy, Heilongjiang University of Traditional Chinese Medicine, Harbin 150040, Heilongjiang, China.
authors.forename: Haixue
authors.initials: H
authors.lastname: Kuang
mesh_terms: D000818:Animals; D001599:Berberine / Q000032:analysis / Q000097:blood; D001600:Berberine Alkaloids / Q000032:analysis / Q000097:blood; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003093:Colitis, Ulcerative / Q000097:blood*; D004365:Drugs, Chinese Herbal / Q000032:analysis* / Q000378:metabolism*; D004929:Esculin / Q000032:analysis / Q000097:blood; D008297:Male; D011807:Quinolizines / Q000032:analysis / Q000097:blood; D051381:Rats; D017207:Rats, Sprague-Dawley; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D053719:Tandem Mass Spectrometry / Q000379:methods; D014468:Umbelliferones / Q000032:analysis / Q000097:blood
chemical_list: D001600:Berberine Alkaloids; D004365:Drugs, Chinese Herbal; D011807:Quinolizines; D014468:Umbelliferones; D001599:Berberine; D004929:Esculin; C079418:phellodendrine; C005413:palmatine; C007628:esculetin
keywords: Compare the pharmacokinetics; Multiple reaction monitoring; Pulsatillae Decoction; UPLC-ESI–MS/MS; Ulcerative colitis