title: Piperine attenuates lipopolysaccharide (LPS)-induced inflammatory responses in BV2 microglia.
abstract: Piperine, the chief alkaloid isolated from Piper nigrum, has been known to have anti-inflammatory effect. However, the effects of piperine on neuroinflammation have not been reported. In the present study, we evaluated the effects of piperine on neuroinflammation in BV2 microglia and investigated the molecular mechanism. The results showed that piperine significantly inhibited LPS-induced TNF-α, IL-6, IL-1β, and PGE2production in BV2 cells. Western blot analysis showed that piperine dose-dependently inhibited LPS-induced NF-κB activation. Furthermore, piperine was found to amplify the expression of Nrf2 and HO-1 up-regulated by LPS. In addition, the inhibition of inflammatory mediators by piperine can be reversed by transfection with Nrf2 siRNA. In conclusion, piperine inhibited LPS-induced inflammatory response by activating Nrf2 signaling pathway. These results indicated that piperine may be a promising agent for the treatment of neurodegenerative diseases.
authors.affiliation: Department of Radiology, Hainan General Hospital, Haikou 570311, China. Electronic address: chenwangsheng11@163.com.
authors.forename: Chen
authors.initials: C
authors.lastname: Wang-Sheng
authors.affiliation: Department of Medical Imaging, The First Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou 510405, China.
authors.forename: An
authors.initials: A
authors.lastname: Jie
authors.affiliation: Department of Radiology, Hainan General Hospital, Haikou 570311, China.
authors.forename: Li
authors.initials: L
authors.lastname: Jian-Jun
authors.affiliation: Department of Gynecology, Hainan General Hospital, Haikou 570311, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Lan
authors.affiliation: Department of Radiology, Hainan General Hospital, Haikou 570311, China.
authors.forename: Xing
authors.initials: X
authors.lastname: Zeng-Bao
authors.affiliation: Department of Radiology, Hainan General Hospital, Haikou 570311, China.
authors.forename: Li
authors.initials: L
authors.lastname: Chang-Qing
mesh_terms: D000470:Alkaloids / Q000494:pharmacology*; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D052117:Benzodioxoles / Q000494:pharmacology*; D002460:Cell Line; D016207:Cytokines / Q000378:metabolism; D015232:Dinoprostone / Q000378:metabolism; D051547:Heme Oxygenase-1 / Q000378:metabolism; D018836:Inflammation Mediators / Q000378:metabolism; D008070:Lipopolysaccharides / Q000276:immunology; D008565:Membrane Proteins / Q000378:metabolism; D051379:Mice; D017628:Microglia / Q000187:drug effects* / Q000276:immunology; D051267:NF-E2-Related Factor 2 / Q000235:genetics / Q000378:metabolism*; D016328:NF-kappa B / Q000378:metabolism; D029222:Piper nigrum / Q000276:immunology*; D010880:Piperidines / Q000494:pharmacology*; D053284:Polyunsaturated Alkamides / Q000494:pharmacology*; D034741:RNA, Small Interfering / Q000235:genetics
chemical_list: D000470:Alkaloids; D000893:Anti-Inflammatory Agents; D052117:Benzodioxoles; D016207:Cytokines; D018836:Inflammation Mediators; D008070:Lipopolysaccharides; D008565:Membrane Proteins; D051267:NF-E2-Related Factor 2; D016328:NF-kappa B; C495636:Nfe2l2 protein, mouse; D010880:Piperidines; D053284:Polyunsaturated Alkamides; D034741:RNA, Small Interfering; D051547:Heme Oxygenase-1; C496542:Hmox1 protein, mouse; D015232:Dinoprostone; C008922:piperine
keywords: Inflammatory mediators; LPS; NF-κB; Nrf2; Piperine