title: Diosgenin-caused changes of the adrenal gland histological parameters in a rat model of the menopause.
abstract: Diosgenin, a steroidal sapogenin of natural origin, has demonstrated benefits when it comes to the treatment of malignancies, cardiovascular issues and menopausal symptoms. In this study, we investigated the histological changes of the adrenal gland after diosgenin application in a rat model of the menopause. Middle-aged, acyclic female Wistar rats were divided into control (C; n=6) and diosgenin treated (D; n=6) groups. Diosgenin (100mg/kg b.w./day) was orally administered for four weeks, while C group received the vehicle alone. A histological approach included design-based stereology, histochemistry and immunohistochemistry. The adrenal cortex volume decreased in D females by 15% (p<0.05) while the volume of adrenal medulla increased (p<0.05) by 64%, compared to the same parameters in C group. Volume density of the zona glomerulosa (expressed per absolute adrenal gland volume) in D rats increased (p<0.05) by 22% in comparison with C animals. Diosgenin treatment decreased (p<0.05) the volume density of the zona fasciculata (expressed per volume of adrenal cortex) by 15% when compared to C females. Absolute volume of the zona reticularis in D group decreased (p<0.05) by 38% in comparison with the same parameter in C rats. Also, after diosgenin application, the volume density of the zona reticularis (expressed per volume of adrenal cortex) and the zona reticularis cell volume were decreased by 51% and 20% (p<0.05) respectively, compared to C animals. Our results, reflecting a decrease in many stereological parameters of the adrenal cortex, indicate that diosgenin took over the role of corticosteroid precursors and became incorporated into steroidogenesis.
authors.affiliation: Department of Cytology, Institute for Biological Research "Siniša Stanković", University of Belgrade, Despot Stefan Blvd. 142, 11060 Belgrade, Serbia. Electronic address: avlada@ibiss.bg.ac.rs.
authors.forename: Vladimir
authors.initials: V
authors.lastname: Ajdžanović
authors.affiliation: Department of Cytology, Institute for Biological Research "Siniša Stanković", University of Belgrade, Despot Stefan Blvd. 142, 11060 Belgrade, Serbia.
authors.forename: Ivana
authors.initials: I
authors.lastname: Jarić
authors.affiliation: Department of Cytology, Institute for Biological Research "Siniša Stanković", University of Belgrade, Despot Stefan Blvd. 142, 11060 Belgrade, Serbia.
authors.forename: Marko
authors.initials: M
authors.lastname: Miler
authors.affiliation: Department of Cytology, Institute for Biological Research "Siniša Stanković", University of Belgrade, Despot Stefan Blvd. 142, 11060 Belgrade, Serbia.
authors.forename: Branko
authors.initials: B
authors.lastname: Filipović
authors.affiliation: Department of Cytology, Institute for Biological Research "Siniša Stanković", University of Belgrade, Despot Stefan Blvd. 142, 11060 Belgrade, Serbia.
authors.forename: Branka
authors.initials: B
authors.lastname: Šošić-Jurjević
authors.affiliation: Department of Cytology, Institute for Biological Research "Siniša Stanković", University of Belgrade, Despot Stefan Blvd. 142, 11060 Belgrade, Serbia.
authors.forename: Nataša
authors.initials: N
authors.lastname: Ristić
authors.affiliation: Unité de Nutrition Humaine, INRA, UMR 1019, UNH, CRNH Auvergne, Clermont Université, Université d'Auvergne, BP 10448, F-63000 Clermont-Ferrand, France.
authors.forename: Dragan
authors.initials: D
authors.lastname: Milenkovic
authors.affiliation: Department of Cytology, Institute for Biological Research "Siniša Stanković", University of Belgrade, Despot Stefan Blvd. 142, 11060 Belgrade, Serbia.
authors.forename: Verica
authors.initials: V
authors.lastname: Milošević
mesh_terms: D000284:Administration, Oral; D000302:Adrenal Cortex / Q000187:drug effects* / Q000648:ultrastructure; D000313:Adrenal Medulla / Q000187:drug effects* / Q000648:ultrastructure; D000818:Animals; D004144:Diosgenin / Q000494:pharmacology*; D005260:Female; D006801:Humans; D008593:Menopause / Q000187:drug effects* / Q000502:physiology; D023421:Models, Animal; D051381:Rats; D017208:Rats, Wistar; D013256:Steroids / Q000494:pharmacology*
chemical_list: D013256:Steroids; D004144:Diosgenin
keywords: Adrenal gland; Diosgenin; Histology; Menopause; Rats