title: Synthesis of precipitating chromogenic/fluorogenic β-glucosidase/β-galactosidase substrates by a new method and their application in the visual detection of foodborne pathogenic bacteria.
abstract: We developed a new efficient method for the synthesis of important indoxyl glycoside substrates for β-glucosidase and β-galactosidase by using 1-acetylindol-3-ones as intermediates. This method was used to synthesise novel precipitating fluorogenic substrates for β-glucosidase based on 2-(benzothiazol-2'-yl)-phenols. We also assessed the application of these substrates in the detection of foodborne pathogenic bacteria.
authors.affiliation: Guangzhou Institute of Chemistry, Chinese Academy of Sciences, Guangzhou 510650, China and Guangdong Institute of Microbiology, State Key Laboratory of Applied Microbiology Southern China, Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangdong Open Laboratory of Applied Microbiology, Guangzhou 510070, China. wuqp203@163.com and University of Chinese Academy of Sciences, Beijing 100039, China.
authors.forename: Xianhu
authors.initials: X
authors.lastname: Wei
authors.affiliation: Guangdong Institute of Microbiology, State Key Laboratory of Applied Microbiology Southern China, Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangdong Open Laboratory of Applied Microbiology, Guangzhou 510070, China. wuqp203@163.com.
authors.forename: Qingping
authors.initials: Q
authors.lastname: Wu
authors.affiliation: Guangdong Institute of Microbiology, State Key Laboratory of Applied Microbiology Southern China, Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangdong Open Laboratory of Applied Microbiology, Guangzhou 510070, China. wuqp203@163.com.
authors.forename: Jumei
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Guangzhou Institute of Chemistry, Chinese Academy of Sciences, Guangzhou 510650, China and Guangdong Institute of Microbiology, State Key Laboratory of Applied Microbiology Southern China, Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangdong Open Laboratory of Applied Microbiology, Guangzhou 510070, China. wuqp203@163.com and University of Chinese Academy of Sciences, Beijing 100039, China.
authors.forename: Youxiong
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Guangdong Institute of Microbiology, State Key Laboratory of Applied Microbiology Southern China, Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangdong Open Laboratory of Applied Microbiology, Guangzhou 510070, China. wuqp203@163.com.
authors.forename: Weipeng
authors.initials: W
authors.lastname: Guo
authors.affiliation: Guangdong Institute of Microbiology, State Key Laboratory of Applied Microbiology Southern China, Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangdong Open Laboratory of Applied Microbiology, Guangzhou 510070, China. wuqp203@163.com.
authors.forename: Moutong
authors.initials: M
authors.lastname: Chen
authors.affiliation: Guangdong Institute of Microbiology, State Key Laboratory of Applied Microbiology Southern China, Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangdong Open Laboratory of Applied Microbiology, Guangzhou 510070, China. wuqp203@163.com and School of Bioscience and Bioengineering, South China University of Technology, Guangzhou 510006, China.
authors.forename: Qihui
authors.initials: Q
authors.lastname: Gu
authors.affiliation: Guangdong Huankai Microbial Sci. & Tech. Co., Ltd, Guangzhou 510663, China.
authors.forename: Zhihe
authors.initials: Z
authors.lastname: Cai
authors.affiliation: Guangdong Huankai Microbial Sci. & Tech. Co., Ltd, Guangzhou 510663, China.
authors.forename: Mianfei
authors.initials: M
authors.lastname: Lu
mesh_terms: D001419:Bacteria / Q000201:enzymology / Q000302:isolation & purification*; D060326:Chemistry Techniques, Synthetic; D005456:Fluorescent Dyes / Q000138:chemical synthesis* / Q000737:chemistry / Q000378:metabolism*; D005516:Food Microbiology; D006027:Glycosides / Q000138:chemical synthesis* / Q000737:chemistry / Q000378:metabolism*; D007211:Indoles / Q000737:chemistry; D001616:beta-Galactosidase / Q000378:metabolism*; D001617:beta-Glucosidase / Q000378:metabolism*
chemical_list: D005456:Fluorescent Dyes; D006027:Glycosides; D007211:Indoles; D001617:beta-Glucosidase; D001616:beta-Galactosidase