title: Generation of induced pluripotent stem cells with high efficiency from human embryonic renal cortical cells.
abstract: Reprogramming of somatic cells into induced pluripotent stem cells (iPSCs) emerges as a prospective therapeutic angle in regenerative medicine and a tool for drug screening. Although increasing numbers of iPSCs from different sources have been generated, there has been limited progress in yield of iPSC. Here, we show that four Yamanaka factors Oct4, Sox2, Klf4 and c-Myc can convert human embryonic renal cortical cells (hERCCs) to pluripotent stem cells with a roughly 40-fold higher reprogramming efficiency compared with that of adult human dermal fibroblasts. These iPSCs show pluripotency in vitro and in vivo, as evidenced by expression of pluripotency associated genes, differentiation into three embryonic germ layers by teratoma tests, as well as neuronal fate specification by embryoid body formation. Moreover, the four exogenous genes are effectively silenced in these iPSCs. This study highlights the use of hERCCs to generate highly functional human iPSCs which may aid the study of genetic kidney diseases and accelerate the development of cell-based regenerative therapy.
authors.affiliation: The Lab of Traditional Chinese Medicine, Chongqing Medical University Chongqing 400016, China.
authors.forename: Ling
authors.initials: L
authors.lastname: Yao
authors.affiliation: Department of Obstetrics & Gynecology, Obstetrics & Gynecology Hospital, Fudan University Shanghai 200011, China.
authors.forename: Ruifang
authors.initials: R
authors.lastname: Chen
authors.affiliation: Department of Neurosurgery, Huashan Hospital, Fudan University Shanghai 200040, China.
authors.forename: Pu
authors.initials: P
authors.lastname: Wang
authors.affiliation: Department of Dermatology, Huashan Hospital, Fudan University Shanghai 200040, China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: Department of Neurosurgery, Huashan Hospital, Fudan University Shanghai 200040, China.
authors.forename: Hailiang
authors.initials: H
authors.lastname: Tang
authors.affiliation: Department of Radiology, Huashan Hospital, Fudan University Shanghai 200040, China.
authors.forename: Huaping
authors.initials: H
authors.lastname: Sun
keywords: Induced pluripotent stem cells (iPSCs); human embryonic renal cortical cells (hERCCs); reprogramming