title: Ionizing radiation promotes CCL27 secretion from keratinocytes through the cross talk between TNF-α and ROS.
abstract: The skin-associated chemokine CCL27 and its receptor CCR10 mediate the immune response of skin-homing T cells. The CCL27 secreted from keratinocytes was reportedly involved in inflammatory skin diseases such as atopic dermatitis, contact dermatitis, and psoriasis. However, whether ionizing radiation increases the levels of CCL27 secretion still remains unclear. In HaCaT cells, a human keratinocyte cell line, CCL27 secretion was markedly increased after X-ray irradiation. We further found that irradiation boosted the generation of reactive oxygen species (ROS), which was concomitant with the release of tumor necrosis factor-alpha (TNF-α). Moreover, alteration of ROS in irradiated HaCaT cells correlated with TNF-α secretion, indicating a positive loop of TNF-α secretion and ROS generation. This positive loop regulated the secretion of CCL27 from irradiated cells. We therefore concluded that the cross talk between TNF-α and ROS after keratinocytes was exposed to radiation, triggered CCL27 secretion for subsequent inflammation response.
authors.affiliation: Teaching & Research Section of Nuclear Medicine, Anhui Medical University, Hefei, Anhui, 230032, People's Republic of China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: Teaching & Research Section of Nuclear Medicine, Anhui Medical University, Hefei, Anhui, 230032, People's Republic of China.
authors.forename: Linlin
authors.initials: L
authors.lastname: Zhu
authors.affiliation: Teaching & Research Section of Nuclear Medicine, Anhui Medical University, Hefei, Anhui, 230032, People's Republic of China.
authors.forename: Gang
authors.initials: G
authors.lastname: Wang
authors.affiliation: Teaching & Research Section of Nuclear Medicine, Anhui Medical University, Hefei, Anhui, 230032, People's Republic of China.
authors.forename: Ye
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: Teaching & Research Section of Nuclear Medicine, Anhui Medical University, Hefei, Anhui, 230032, People's Republic of China.|Center for Molecular Immunology and Infectious Disease, Department of Veterinary and Biomedical Sciences, The Pennsylvania State University, University Park, PA, 16802, USA.
authors.forename: Na
authors.initials: N
authors.lastname: Xiong
authors.affiliation: Teaching & Research Section of Nuclear Medicine, Anhui Medical University, Hefei, Anhui, 230032, People's Republic of China.
authors.forename: Hegang
authors.initials: H
authors.lastname: Bao
authors.affiliation: Teaching & Research Section of Nuclear Medicine, Anhui Medical University, Hefei, Anhui, 230032, People's Republic of China.
authors.forename: Wensen
authors.initials: W
authors.lastname: Jin
mesh_terms: D054425:Chemokine CCL27 / Q000378:metabolism*; D003877:Dermatitis, Contact / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D006801:Humans; D007111:Immunity, Cellular / Q000187:drug effects*; D007249:Inflammation / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D015603:Keratinocytes / Q000378:metabolism / Q000528:radiation effects; D016328:NF-kappa B / Q000378:metabolism; D011565:Psoriasis / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D011839:Radiation, Ionizing; D017382:Reactive Oxygen Species / Q000378:metabolism*; D054447:Receptors, CCR10 / Q000378:metabolism; D013601:T-Lymphocytes / Q000276:immunology / Q000528:radiation effects; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism*
chemical_list: C492276:CCL27 protein, human; C515706:CCR10 protein, human; D054425:Chemokine CCL27; D016328:NF-kappa B; D017382:Reactive Oxygen Species; D054447:Receptors, CCR10; D014409:Tumor Necrosis Factor-alpha
keywords: CCL27; Ionizing radiation; keratinocyte; reactive oxygen species; tumor necrosis factor-alpha (TNF-α)