title: L-Methionine Production.
abstract: L-Methionine has been used in various industrial applications such as the production of feed and food additives and has been used as a raw material for medical supplies and drugs. It functions not only as an essential amino acid but also as a physiological effector, for example, by inhibiting fat accumulation and enhancing immune response. Producing methionine from fermentation is beneficial in that microorganisms can produce L-methionine selectively using eco-sustainable processes. Nevertheless, the fermentative method has not been used on an industrial scale because it is not competitive economically compared with chemical synthesis methods. Presented are efforts to develop suitable strains, engineered enzymes, and alternative process of producing L-methionine that overcomes problems of conventional fermentation methods. One of the alternative processes is a two-step process in which the L-methionine precursor is produced by fermentation and then converted to L-methionine by enzymes. Directed efforts toward strain development and enhanced enzyme engineering will advance industrial production of L-methionine based on fermentation.
authors.affiliation: Research Institute of Biotechnology, CJ CheilJedang Corp., CJ Blossom Park. 55, Gwanggyo-ro 42beon-gil, Yeongtong-gu, Suwon-si, Gyeonggi-do, 16495, South Korea.
authors.forename: Jihyun
authors.initials: J
authors.lastname: Shim
authors.affiliation: Research Institute of Biotechnology, CJ CheilJedang Corp., CJ Blossom Park. 55, Gwanggyo-ro 42beon-gil, Yeongtong-gu, Suwon-si, Gyeonggi-do, 16495, South Korea.
authors.forename: Yonguk
authors.initials: Y
authors.lastname: Shin
authors.affiliation: Research Institute of Biotechnology, CJ CheilJedang Corp., CJ Blossom Park. 55, Gwanggyo-ro 42beon-gil, Yeongtong-gu, Suwon-si, Gyeonggi-do, 16495, South Korea.
authors.forename: Imsang
authors.initials: I
authors.lastname: Lee
authors.affiliation: Research Institute of Biotechnology, CJ CheilJedang Corp., CJ Blossom Park. 55, Gwanggyo-ro 42beon-gil, Yeongtong-gu, Suwon-si, Gyeonggi-do, 16495, South Korea. soyoung.kim@cj.net.
authors.forename: So Young
authors.initials: SY
authors.lastname: Kim
mesh_terms: D000596:Amino Acids / Q000096:biosynthesis* / Q000235:genetics; D018407:Bacterial Physiological Phenomena; D001688:Biological Products / Q000138:chemical synthesis / Q000378:metabolism; D019149:Bioreactors / Q000382:microbiology; D005285:Fermentation / Q000502:physiology*; D005503:Food Additives / Q000378:metabolism*; D024861:Genetic Enhancement / Q000379:methods*; D008715:Methionine / Q000502:physiology*
chemical_list: D000596:Amino Acids; D001688:Biological Products; D005503:Food Additives; D008715:Methionine
keywords: Enzyme engineering; Feed additives; Methionine; Microbial fermentation; Strain development