title: Soft particle analysis of electrokinetics of biological cells and their model systems.
abstract: In this article, we review the applications of a novel theory (Ohshima 2009Sci. Technol. Adv. Mater.10063001) to the analysis of electrokinetic data for various soft particles, that is, particles covered with an ion-permeable surface layer of polyelectrolytes. Soft particles discussed in this review include various biological cells and hydrogel-coated particles as a model of biological cells. Cellular transformations increase the concentration of sialic acid of glycoproteins and are associated with blocked biosynthesis of glycolipids and aberrant expression of the developmentally programmed biosynthetic pathway. The change in shape or biological function of cells may affect their surface properties and can be detected by electrokinetic measurements. The experimental results were analyzed with Ohshima's electrokinetic formula for soft particles and soft surfaces. As a model system, hydrogel surfaces that mimic biological surfaces were also prepared and their surface properties were studied.
authors.affiliation: Faculty of Pharmaceutical Sciences, Tokyo University of Science, 2641 Yamazaki, Noda, Chiba 278-8510, Japan; Center for Colloid and Interface Science, Research Institute for Science and Technology, Tokyo University of Science, 2641 Yamazaki, Noda, Chiba 278-8510, Japan; Center for Physicl Pharmaceutics, Research Institute for Science and Technology, Tokyo University of Science, 2641 Yamazaki, Noda, Chiba 278-8510, Japan.
authors.forename: Kimiko
authors.initials: K
authors.lastname: Makino
authors.affiliation: Faculty of Pharmaceutical Sciences, Tokyo University of Science, 2641 Yamazaki, Noda, Chiba 278-8510, Japan; Center for Colloid and Interface Science, Research Institute for Science and Technology, Tokyo University of Science, 2641 Yamazaki, Noda, Chiba 278-8510, Japan.
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Ohshima
keywords: biological cells; electrokinetics; hydrogel; soft particles; soft surfaces