title: Circular RNA ZNF609 functions as a competitive endogenous RNA to regulate AKT3 expression by sponging miR-150-5p in Hirschsprung's disease.
abstract: Research over the past decade suggested critical roles for circular RNAs in the natural growth and disease progression. However, it remains poorly defined whether the circular RNAs participate in Hirschsprung disease (HSCR). Here, we reported that the cir-ZNF609 was down-regulated in HSCR compared with normal bowel tissues. Furthermore, suppression of cir-ZNF609 inhibited the proliferation and migration of cells. We screened out several putative cir-ZNF609 ceRNAs of which the AKT3 transcript was selected. Finally, RNA immunoprecipitation and luciferase reporter assays demonstrated that cir-ZNF609 may act as a sponge for miR-150-5p to modulate the expression of AKT3. In conclusion, these findings illustrated that cir-ZNF609 took part in the onset of HSCR through the crosstalk with AKT3 by competing for shared miR-150-5p.
authors.affiliation: State Key Laboratory of Reproductive Medicine, Institute of Toxicology, School of Public Health, Nanjing Medical University, Nanjing, China.|Department of Pediatric Surgery, Children's Hospital of Nanjing Medical University, Nanjing, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Peng
authors.affiliation: State Key Laboratory of Reproductive Medicine, Institute of Toxicology, School of Public Health, Nanjing Medical University, Nanjing, China.|Department of Pediatric Surgery, Children's Hospital of Nanjing Medical University, Nanjing, China.
authors.forename: Guanglin
authors.initials: G
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Reproductive Medicine, Institute of Toxicology, School of Public Health, Nanjing Medical University, Nanjing, China.|Department of Pediatric Surgery, Children's Hospital of Nanjing Medical University, Nanjing, China.
authors.forename: Zhongxian
authors.initials: Z
authors.lastname: Zhu
authors.affiliation: State Key Laboratory of Reproductive Medicine, Institute of Toxicology, School of Public Health, Nanjing Medical University, Nanjing, China.|Department of Pediatric Surgery, Children's Hospital of Nanjing Medical University, Nanjing, China.
authors.forename: Ziyang
authors.initials: Z
authors.lastname: Shen
authors.affiliation: State Key Laboratory of Reproductive Medicine, Institute of Toxicology, School of Public Health, Nanjing Medical University, Nanjing, China.|Department of Pediatric Surgery, Children's Hospital of Nanjing Medical University, Nanjing, China.
authors.forename: Chunxia
authors.initials: C
authors.lastname: Du
authors.affiliation: State Key Laboratory of Reproductive Medicine, Institute of Toxicology, School of Public Health, Nanjing Medical University, Nanjing, China.|Department of Pediatric Surgery, Children's Hospital of Nanjing Medical University, Nanjing, China.
authors.forename: Rujin
authors.initials: R
authors.lastname: Zang
authors.affiliation: State Key Laboratory of Reproductive Medicine, Institute of Toxicology, School of Public Health, Nanjing Medical University, Nanjing, China.|Department of Pediatric Surgery, Children's Hospital of Nanjing Medical University, Nanjing, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Su
authors.affiliation: State Key Laboratory of Reproductive Medicine, Institute of Toxicology, School of Public Health, Nanjing Medical University, Nanjing, China.|Department of Pediatric Surgery, Children's Hospital of Nanjing Medical University, Nanjing, China.
authors.forename: Hua
authors.initials: H
authors.lastname: Xie
authors.affiliation: State Key Laboratory of Reproductive Medicine, Institute of Toxicology, School of Public Health, Nanjing Medical University, Nanjing, China.|Department of Pediatric Surgery, Children's Hospital of Nanjing Medical University, Nanjing, China.
authors.forename: Hongxing
authors.initials: H
authors.lastname: Li
authors.affiliation: State Key Laboratory of Reproductive Medicine, Institute of Toxicology, School of Public Health, Nanjing Medical University, Nanjing, China.|Department of Pediatric Surgery, Children's Hospital of Nanjing Medical University, Nanjing, China.
authors.forename: Xiaoqun
authors.initials: X
authors.lastname: Xu
authors.affiliation: State Key Laboratory of Reproductive Medicine, Institute of Toxicology, School of Public Health, Nanjing Medical University, Nanjing, China.|Key Laboratory of Modern Toxicology, Nanjing Medical University, Ministry of Education, China.
authors.forename: Yankai
authors.initials: Y
authors.lastname: Xia
authors.affiliation: State Key Laboratory of Reproductive Medicine, Institute of Toxicology, School of Public Health, Nanjing Medical University, Nanjing, China.|Department of Pediatric Surgery, Children's Hospital of Nanjing Medical University, Nanjing, China.
authors.forename: Weibing
authors.initials: W
authors.lastname: Tang
mesh_terms: D001483:Base Sequence; D016022:Case-Control Studies; D002460:Cell Line; D002465:Cell Movement / Q000235:genetics; D049109:Cell Proliferation; D005260:Female; D015870:Gene Expression; D005786:Gene Expression Regulation; D017930:Genes, Reporter; D006627:Hirschsprung Disease / Q000235:genetics*; D006801:Humans; D007223:Infant; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D051057:Proto-Oncogene Proteins c-akt / Q000235:genetics*; D012313:RNA / Q000235:genetics*; D034443:RNA Transport; D000079962:RNA, Circular; D017423:Sequence Analysis, RNA
chemical_list: D035683:MicroRNAs; D051057:Proto-Oncogene Proteins c-akt; D012313:RNA; D000079962:RNA, Circular; C533097:MIR150, human; C491754:AKT3 protein, human
keywords: circular RNA (circRNA); competing endogenous RNAs (ceRNA); hirschsprung disease; migration; proliferation