title: Enterococcus faeciumHDRsEf1 Protects the Intestinal Epithelium and Attenuates ETEC-Induced IL-8 Secretion in Enterocytes.
abstract: The probioticEnterococcus faeciumHDRsEf1 (Ef1) has been shown to have positive effects on piglet diarrhoea, but the mechanism has not yet been elucidated. In this study, using the IPEC-J2 cell line to mimic intestinal epithelial cells and enterotoxigenicEscherichia coli(ETEC) K88ac as a representative intestinal pathogen, the mechanism underlying Ef1 protection against an enteropathogen was investigated. The results demonstrated that Ef1 was effective in displacing K88ac from the IPEC-J2 cell layer. Moreover, Ef1 and its cell-free supernatant (S-Ef1) modulate IL-8 released by IPEC-J2 cells. Ef1 and its cell-free supernatant showed the potential to protect enterocytes from an acute inflammatory response. In addition, Ef1 and its cell-free supernatant increased the transepithelial electrical resistance (TEER) of the enterocyte monolayer, thus strengthening the intestinal barrier against ETEC. These results may contribute to the development of therapeutic interventions using Ef1 in intestinal disorders of piglets.
authors.affiliation: State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China; Key Laboratory of Development of Veterinary Diagnostic Products, Ministry of Agriculture, Huazhong Agricultural University, Wuhan, Hubei 430070, China.
authors.forename: Zhongyuan
authors.identifier: 0000-0002-5986-2583
authors.initials: Z
authors.lastname: Tian
authors.affiliation: State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China; Key Laboratory of Development of Veterinary Diagnostic Products, Ministry of Agriculture, Huazhong Agricultural University, Wuhan, Hubei 430070, China.
authors.forename: Xiaofang
authors.identifier: 0000-0001-5338-4798
authors.initials: X
authors.lastname: Liu
authors.affiliation: College of Animal Science and Technology, Agricultural University of Hebei, Baoding 071000, China.
authors.forename: Ran
authors.identifier: 0000-0001-5158-8623
authors.initials: R
authors.lastname: Dai
authors.affiliation: State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China; Key Laboratory of Development of Veterinary Diagnostic Products, Ministry of Agriculture, Huazhong Agricultural University, Wuhan, Hubei 430070, China.
authors.forename: Yuncai
authors.initials: Y
authors.lastname: Xiao
authors.affiliation: State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China; Key Laboratory of Development of Veterinary Diagnostic Products, Ministry of Agriculture, Huazhong Agricultural University, Wuhan, Hubei 430070, China.
authors.forename: Xiliang
authors.initials: X
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China; Key Laboratory of Development of Veterinary Diagnostic Products, Ministry of Agriculture, Huazhong Agricultural University, Wuhan, Hubei 430070, China.
authors.forename: Dingren
authors.initials: D
authors.lastname: Bi
authors.affiliation: State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China; Key Laboratory of Development of Veterinary Diagnostic Products, Ministry of Agriculture, Huazhong Agricultural University, Wuhan, Hubei 430070, China.
authors.forename: Deshi
authors.identifier: 0000-0002-9753-5700
authors.initials: D
authors.lastname: Shi
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000378:metabolism; D002460:Cell Line; D016984:Enterococcus faecium / Q000502:physiology*; D020895:Enterocytes / Q000276:immunology / Q000378:metabolism* / Q000382:microbiology*; D054307:Enterotoxigenic Escherichia coli / Q000276:immunology* / Q000472:pathogenicity*; D007249:Inflammation / Q000276:immunology / Q000378:metabolism / Q000382:microbiology; D016209:Interleukin-8 / Q000378:metabolism*; D007413:Intestinal Mucosa / Q000276:immunology / Q000378:metabolism* / Q000382:microbiology*; D019936:Probiotics; D013552:Swine
chemical_list: D001426:Bacterial Proteins; D016209:Interleukin-8