title: Migration and Proliferative Activity of Mesenchymal Stem Cells in 3D Polylactide Scaffolds Depends on Cell Seeding Technique and Collagen Modification.
abstract: We analyzed viability of mesenchymal stem cells seeded by static and dynamic methods to highly porous fibrous 3D poly-L-lactide scaffolds with similar physical and chemical properties, but different spatial organization modified with collagen. Standard collagen coating promoted protein adsorption on the scaffold surface and improved adhesive properties of 100 μ-thick scaffolds. Modification of 600-μ scaffolds with collagen under pressure increased proliferative activity of mesenchymal stem cells seeded under static and dynamic (delivery of 100,000 cells in 10 ml medium in a perfusion system at a rate of 1 ml/min) conditions by 47 and 648%, respectively (measured after 120-h culturing by MTT test). Dynamic conditions provide more uniform distribution of collagen on scaffold fibers and promote cell penetration into 3D poly-L-lactide scaffolds with thickness >600 μ.
authors.affiliation: National Research Center Kurchatov Institute, Moscow, Russia. rodina.allav@gmail.com.
authors.forename: A V
authors.initials: AV
authors.lastname: Rodina
authors.affiliation: National Research Center Kurchatov Institute, Moscow, Russia.
authors.forename: T Kh
authors.initials: TK
authors.lastname: Tenchurin
authors.affiliation: A.I. Burnazyan Federal Medical and Biophysical Center, Moscow, Russia.
authors.forename: V P
authors.initials: VP
authors.lastname: Saprykin
authors.affiliation: National Research Center Kurchatov Institute, Moscow, Russia.
authors.forename: A D
authors.initials: AD
authors.lastname: Shepelev
authors.affiliation: National Research Center Kurchatov Institute, Moscow, Russia.
authors.forename: V G
authors.initials: VG
authors.lastname: Mamagulashvili
authors.affiliation: National Research Center Kurchatov Institute, Moscow, Russia.
authors.forename: T E
authors.initials: TE
authors.lastname: Grigor'ev
authors.affiliation: National Research Center Kurchatov Institute, Moscow, Russia.
authors.forename: K I
authors.initials: KI
authors.lastname: Lukanina
authors.affiliation: National Research Center Kurchatov Institute, Moscow, Russia.
authors.forename: A S
authors.initials: AS
authors.lastname: Orekhov
authors.affiliation: National Research Center Kurchatov Institute, Moscow, Russia.
authors.forename: E Yu
authors.initials: EY
authors.lastname: Moskaleva
authors.affiliation: National Research Center Kurchatov Institute, Moscow, Russia.
authors.forename: S N
authors.initials: SN
authors.lastname: Chvalun
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology / Q000187:drug effects* / Q000502:physiology; D002448:Cell Adhesion / Q000187:drug effects; D018929:Cell Culture Techniques; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D020099:Coated Materials, Biocompatible / Q000737:chemistry / Q000494:pharmacology*; D003094:Collagen / Q000737:chemistry / Q000494:pharmacology; D005452:Fluoresceins; D005456:Fluorescent Dyes; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000187:drug effects* / Q000502:physiology; D051379:Mice; D008810:Mice, Inbred C57BL; D011091:Polyesters / Q000737:chemistry / Q000494:pharmacology*; D016062:Porosity; D061251:Primary Cell Culture; D011419:Propidium; D012212:Rheology; D023822:Tissue Engineering; D054457:Tissue Scaffolds
chemical_list: D020099:Coated Materials, Biocompatible; D005452:Fluoresceins; D005456:Fluorescent Dyes; D011091:Polyesters; D011419:Propidium; C033616:poly(lactide); D003094:Collagen; C018506:diacetylfluorescein
keywords: collagen; mesenchymal stromal cells; microfibrous scaffolds; polylactide; tissue engineering