title: Three-generation reproduction toxicity study of genetically modified rice with insect resistant genes.
abstract: In the present work, we evaluated the three generation reproductive toxicity of the genetically modified rice with insectresistant cry1Ac and sck genes. 120 Sprague-Dawley (SD) rats were divided into three groups which were fed with genetically modified rice diet (GM group), parental control rice diet (PR group) and AIN-93 control diet (both used as negative control) respectively. Bodyweight, food consumption, reproductive data, hematological parameters, serum chemistry, relative organ weights and histopathology for each generation were examined respectively. All the hematology and serum chemistry parameters, organ/body weight indicators were within the normal range or no change to the adverse direction was observed, although several differences in hematology and serum chemistry parameters (WBC, BUN, LDH of male rat, PLT, PCT, MPV of female rats), reproductive data (rate of morphologically abnormal sperm) were observed between GM rice group and two control groups. No macroscopic or histological adverse effects were found or considered as treatment-related, either. Overall, the three generation study of genetically modified rice with cry1Ac and sck genes at a high level showed no unintended adverse effects on rats's reproductive system.
authors.affiliation: Key Laboratory of Trace Element Nutrition of National Health and Family Planning Commission, National Institute for Nutrition and Health, Chinese Center for Disease Control and Prevention (China CDC), Beijing 100050, China.
authors.forename: Yichun
authors.initials: Y
authors.lastname: Hu
authors.affiliation: Key Laboratory of Trace Element Nutrition of National Health and Family Planning Commission, National Institute for Nutrition and Health, Chinese Center for Disease Control and Prevention (China CDC), Beijing 100050, China. Electronic address: zhuoqin1@sina.com.
authors.forename: Qin
authors.initials: Q
authors.lastname: Zhuo
authors.affiliation: Key Laboratory of Trace Element Nutrition of National Health and Family Planning Commission, National Institute for Nutrition and Health, Chinese Center for Disease Control and Prevention (China CDC), Beijing 100050, China.
authors.forename: Zhaolong
authors.initials: Z
authors.lastname: Gong
authors.affiliation: Key Laboratory of Trace Element Nutrition of National Health and Family Planning Commission, National Institute for Nutrition and Health, Chinese Center for Disease Control and Prevention (China CDC), Beijing 100050, China.
authors.forename: Jianhua
authors.initials: J
authors.lastname: Piao
authors.affiliation: Key Laboratory of Trace Element Nutrition of National Health and Family Planning Commission, National Institute for Nutrition and Health, Chinese Center for Disease Control and Prevention (China CDC), Beijing 100050, China. Electronic address: xgyangcdc@vip.sina.com.
authors.forename: Xiaoguang
authors.initials: X
authors.lastname: Yang
mesh_terms: D000818:Animals; D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins / Q000235:genetics*; D004731:Endotoxins / Q000235:genetics*; D005260:Female; D006460:Hemolysin Proteins / Q000235:genetics*; D007306:Insecticides / Q000633:toxicity*; D008297:Male; D009929:Organ Size / Q000187:drug effects; D012275:Oryza / Q000235:genetics / Q000633:toxicity*; D030821:Plants, Genetically Modified / Q000633:toxicity*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012098:Reproduction / Q000187:drug effects*
chemical_list: D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins; D004731:Endotoxins; D006460:Hemolysin Proteins; D007306:Insecticides; C030956:insecticidal crystal protein, Bacillus Thuringiensis
keywords: Feeding study; Genetically modified rice; Three generation study; Toxicology; cry1Ac; sck genes