title: Potential immunological markers for diagnosis of human strongyloidiasis using heterologous antigens.
abstract: Strongyloides venezuelensis is a parasitic nematode of rodents that is frequently used to obtain heterologous antigens for immunological diagnosis of human strongyloidiasis. The aim of this study was to identify antigens from filariform larvae of S. venezuelensis for immunodiagnosis of human strongyloidiasis. Soluble and membrane fractions from filariform larvae of S. venezuelensis were obtained in phosphate saline (SS and SM) and in Tris-HCl buffer (TS and TM), and were analysed by Western blotting. Different antigenic components were recognized by IgG antibodies from the sera of strongyloidiasis patients. Highest recognition was observed for a 30-40 kDa mass range present in all antigenic fractions. The band encompassing this mass range was then excised and subjected to mass spectrometry for protein identification. Immunoreactive proteins identified in the soluble fractions corresponded to metabolic enzymes, whereas cytoskeletal proteins and galectins were more abundant in the membrane fractions. These results represent the first approach towards identification of S. venezuelensis antigens for use in immunodiagnostic assays for human strongyloidiasis.
authors.affiliation: Laboratório de Investigação Médica (LIM-06),Hospital das Clínicas da Faculdade de Medicina,Universidade de São Paulo,SP,Brazil.
authors.forename: M A
authors.initials: MA
authors.lastname: Corral
authors.affiliation: Laboratório de Investigação Médica (LIM-06),Hospital das Clínicas da Faculdade de Medicina,Universidade de São Paulo,SP,Brazil.
authors.forename: F M
authors.initials: FM
authors.lastname: Paula
authors.affiliation: Laboratório de Investigação Médica (LIM-06),Hospital das Clínicas da Faculdade de Medicina,Universidade de São Paulo,SP,Brazil.
authors.forename: D M C L
authors.initials: DM
authors.lastname: Meisel
authors.affiliation: Divisão de Laboratório Central,Hospital das Clínicas,Faculdade de Medicina,Universidade de São Paulo,São Paulo,SP,Brazil.
authors.forename: V L P
authors.initials: VL
authors.lastname: Castilho
authors.affiliation: Divisão de Laboratório Central,Hospital das Clínicas,Faculdade de Medicina,Universidade de São Paulo,São Paulo,SP,Brazil.
authors.forename: E M N
authors.initials: EM
authors.lastname: Gonçalves
authors.affiliation: Laboratório de Investigação Médica (LIM-31),Hospital das Clínicas da Faculdade de Medicina,Universidade de São Paulo,São Paulo,SP,Brazil.
authors.forename: D
authors.initials: D
authors.lastname: Levy
authors.affiliation: Laboratório de Investigação Médica (LIM-31),Hospital das Clínicas da Faculdade de Medicina,Universidade de São Paulo,São Paulo,SP,Brazil.
authors.forename: S P
authors.initials: SP
authors.lastname: Bydlowski
authors.affiliation: Faculdade de Ciências Médicas,Santa Casa,São Paulo,SP,Brazil.
authors.forename: P P
authors.initials: PP
authors.lastname: Chieffi
authors.affiliation: Departamento de Ciências Biológicas,Universidade Federal de Ouro Preto,Ouro Preto,MG,Brazil.
authors.forename: W
authors.initials: W
authors.lastname: Castro-Borges
authors.affiliation: Laboratório de Investigação Médica (LIM-06),Hospital das Clínicas da Faculdade de Medicina,Universidade de São Paulo,SP,Brazil.
authors.forename: R C B
authors.initials: RC
authors.lastname: Gryschek
mesh_terms: D000818:Animals; D000947:Antigens, Helminth; D015415:Biomarkers / Q000097:blood; D004797:Enzyme-Linked Immunosorbent Assay; D015801:Helminth Proteins / Q000276:immunology; D006801:Humans; D012680:Sensitivity and Specificity; D013321:Strongyloides / Q000276:immunology*; D013322:Strongyloidiasis / Q000097:blood* / Q000175:diagnosis* / Q000276:immunology
chemical_list: D000947:Antigens, Helminth; D015415:Biomarkers; D015801:Helminth Proteins
keywords: 
                 Strongyloides venezuelensis
              ; actin; antigens; galectin; immunodiagnostic