title: Gene expression profiling of the 8q22-24 position in human breast cancer:TSPYL5,MTDH,ATAD2andCCNE2genes are implicated in oncogenesis, whileWISP1andEXT1genes may predict a risk of metastasis.
abstract: Gene expression profiling has been suggested to predict breast cancer outcome. The prognostic value of the 8q22-24 position in breast cancer remains to be elucidated. The present study evaluated expression patterns of the genes located at this position in metastatic and non-metastatic breast cancer. A total of 85 patients with recurrent/metastatic (n=15) and non-metastatic (n=70) early-stage, estrogen receptor-positive and lymph node-negative breast tumors were included. In addition, 15 normal breast tissue samples were used as controls. Demographic and clinical features were recorded. Subsequently, mRNA copy numbers of exostosin glycosyltransferase 1 (EXT1), WNT1 inducible signaling pathway protein 1 (WISP1), ATPase family, AAA domain containing 2 (ATAD2), TSP-like 5 (TSPYL5), metadherin (MTDH) and cyclin E2 (CCNE2) genes were measured by reverse transcription-quantitative polymerase chain reaction assay. The expression ofEXT1andWISP1exhibited a significant decline in the metastatic breast cancer group compared to the control (P=0.015 and P=0.012, respectively). The expression ofTSPYL5,MTDHandATAD2was significantly decreased in the metastatic (P=0.002, P=0.018 and P=0.016, respectively) and non-metastatic (P=0.038, P=0.045 and P=0.000, respectively) breast cancer groups compared with the control. The expression ofCCNE2in the metastatic and non-metastatic breast cancer groups was significantly increased compared with the control (P=0.002 and P=0.001, respectively).WISP1expression demonstrated a correlation with patient age and tumor size, andTSPYL5expression was correlated with lymphovascular invasion. None of the genes investigated exhibited any correlation with stage and grade of disease. TheTSPYL5,MTDH,ATAD2andCCNE2genes may be implicated in the pathogenesis of human breast cancer, while theWISP1andEXT1genes may have the potential to serve as promising indicators of the risk of metastasis. However, further studies are required to validate these results.
authors.affiliation: Department of Cellular and Molecular Biology, Cancer Research Center, Shahid Beheshti University of Medical Sciences, Tehran 1985717413, Iran.
authors.forename: Afsoon
authors.initials: A
authors.lastname: Taghavi
authors.affiliation: Department of Cellular and Molecular Biology, Cancer Research Center, Shahid Beheshti University of Medical Sciences, Tehran 1985717413, Iran.
authors.forename: Mohammad Esmaeil
authors.initials: ME
authors.lastname: Akbari
authors.affiliation: Department of Pathology, Imam Hossein Hospital, Shahid Beheshti University of Medical Sciences, Tehran 1985717413, Iran.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Hashemi-Bahremani
authors.affiliation: Department of Cellular and Molecular Biology, Cancer Research Center, Shahid Beheshti University of Medical Sciences, Tehran 1985717413, Iran.
authors.forename: Nahid
authors.initials: N
authors.lastname: Nafissi
authors.affiliation: Department of Immunology, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran 1985717413, Iran.
authors.forename: Ahad
authors.initials: A
authors.lastname: Khalilnezhad
authors.affiliation: Department of Medical Genetics, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran 1985717413, Iran.
authors.forename: Seyed Mohammad
authors.initials: SM
authors.lastname: Poorhosseini
authors.affiliation: Molecular Diagnostic Laboratory, Genomic Research Center, Shahid Beheshti University of Medical Sciences, Ayatollah Taleghani Educational Hospital, Tehran 1985717413, Iran.
authors.forename: Feyzollah
authors.initials: F
authors.lastname: Hashemi-Gorji
authors.affiliation: Department of Medical Genetics, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran 1985717413, Iran; Molecular Diagnostic Laboratory, Genomic Research Center, Shahid Beheshti University of Medical Sciences, Ayatollah Taleghani Educational Hospital, Tehran 1985717413, Iran.
authors.forename: Vahid Reza
authors.initials: VR
authors.lastname: Yassaee
keywords: breast cancer; gene expression; metastasis