title: Longitudinal study of CTX-M ESBL-producing E. coli strains on a UK dairy farm.
abstract: The aim of this study was to investigate the bacterial strains and farm environment that may contribute to the persistence of ESBL-producing E. coli on a single UK dairy farm. A longitudinal study was conducted comprising 6 visits, between August and October 2010, followed by a further visit at approximately 69weeks after the initial visit. Faecal and environmental samples were collected from different parts of the farm. The persistence and extent of faecal shedding of ESBL E. coli by individual calves was also determined. Twenty two different PFGE types were identified. Four of these were persistent during the study period and were associated with serotypes: O98, O55, O141 and O33. The counts suggest that shedding in calf faeces was an important factor for the persistence of strains, and the data will be useful for parameterising mathematical models of the spread and persistence of ESBL strains within a dairy farm.
authors.affiliation: Animal and Plant Health Agency (APHA) Weybridge, New Haw, Addlestone, Surrey KT15 3NB, United Kingdom. Electronic address: robert.horton@apha.gsi.gov.uk.
authors.forename: R A
authors.initials: RA
authors.lastname: Horton
authors.affiliation: Animal and Plant Health Agency (APHA) Weybridge, New Haw, Addlestone, Surrey KT15 3NB, United Kingdom.
authors.forename: D
authors.initials: D
authors.lastname: Duncan
authors.affiliation: Animal and Plant Health Agency (APHA) Weybridge, New Haw, Addlestone, Surrey KT15 3NB, United Kingdom.
authors.forename: L P
authors.initials: LP
authors.lastname: Randall
authors.affiliation: Animal and Plant Health Agency (APHA) Weybridge, New Haw, Addlestone, Surrey KT15 3NB, United Kingdom.
authors.forename: S
authors.initials: S
authors.lastname: Chappell
authors.affiliation: Animal and Plant Health Agency (APHA) Weybridge, New Haw, Addlestone, Surrey KT15 3NB, United Kingdom.
authors.forename: L A
authors.initials: LA
authors.lastname: Brunton
authors.affiliation: Animal and Plant Health Agency (APHA) Weybridge, New Haw, Addlestone, Surrey KT15 3NB, United Kingdom.
authors.forename: R
authors.initials: R
authors.lastname: Warner
authors.affiliation: Animal and Plant Health Agency (APHA) Weybridge, New Haw, Addlestone, Surrey KT15 3NB, United Kingdom.
authors.forename: N G
authors.initials: NG
authors.lastname: Coldham
authors.affiliation: Animal and Plant Health Agency (APHA) Weybridge, New Haw, Addlestone, Surrey KT15 3NB, United Kingdom.
authors.forename: C J
authors.initials: CJ
authors.lastname: Teale
mesh_terms: D000818:Animals; D057138:Bacterial Shedding; D002417:Cattle; D002418:Cattle Diseases / Q000453:epidemiology* / Q000235:genetics / Q000382:microbiology; D003612:Dairying; D004777:Environment; D004926:Escherichia coli / Q000235:genetics / Q000502:physiology*; D004927:Escherichia coli Infections / Q000453:epidemiology / Q000235:genetics / Q000382:microbiology / Q000662:veterinary*; D029968:Escherichia coli Proteins / Q000032:analysis; D000072480:Farms; D005243:Feces / Q000382:microbiology; D008137:Longitudinal Studies; D008962:Models, Theoretical; D015995:Prevalence; D006113:United Kingdom / Q000453:epidemiology; D001618:beta-Lactamases / Q000032:analysis
chemical_list: D029968:Escherichia coli Proteins; D001618:beta-Lactamases; C000602253:beta-lactamase CTX-M, E coli
keywords: CTX-M ESBLs; E. coli; Farm environment; Strain persistence