title: [Evaluation of an immunochromatographic test for the detection of OXA-48 carbapenemase].
abstract: OBJECTIVE
  Detection and differentiation of various types of carbapenemases is crucial to their control and dissemination. OXA-48 is the most common carbapenemase in Spain and in our environment. The aim of this study is the evaluation of a new immunochromatographic test OXA-48 Card letitest (Coris, BioConcept Belgium) to detect this carbapenemase from solid media.
  
  
  METHODS
  During the last year 151 strains of carbapenemase producing bacteria have been isolated, of which 136 were OXA-48 (126 Klebsiella pneumoniae, 1 Klebsiella oxytoca, 5 Escherichia coli, 4 Enterobacter cloacae), and 15 producing other carbapenemases . These 15 strains with other 73 carrying other resistance mechanisms (54 extended-spectrum β-lactamases producers and 19 with other mechanisms) were used as negative controls.
  
  
  RESULTS
  One hundred and thirty six strains carrying OXA-48 were positive with the test OXA-48 Card letitest and the 88 species used as controls were negative, resulting in a sensitivity and specificity of 100%.
  
  
  CONCLUSIONS
  The OXA-48 Card letitest is simple, quick, safe and cheap (approx. 6€/test) and can be used in microbiology laboratories to confirm the production of OXA-48 carbapenemase in clinical isolates.
authors.forename: C
authors.initials: C
authors.lastname: Mediavilla-Gradolph
authors.forename: R
authors.initials: R
authors.lastname: Sáinz-Rodriguez
authors.forename: M
authors.initials: M
authors.lastname: Valverde-Troya
authors.forename: I
authors.initials: I
authors.lastname: de Toro-Peinado
authors.forename: M P
authors.initials: MP
authors.lastname: Bermudez-Ruíz
authors.affiliation: Begoña Palop-Borrás, Laboratorio de Microbiología, Hospital Regional Universitario de Málaga, Avda. Carlos Haya, s/n 29010 Málaga, Spain. bpalop@hotmail.com.
authors.forename: B
authors.initials: B
authors.lastname: Palop-Borrás
mesh_terms: D001419:Bacteria / Q000187:drug effects / Q000201:enzymology; D001426:Bacterial Proteins / Q000032:analysis*; D015780:Carbapenems / Q000494:pharmacology; D002845:Chromatography; D003470:Culture Media / Q000032:analysis; D029968:Escherichia coli Proteins / Q000032:analysis; D006090:Gram-Negative Bacteria / Q000187:drug effects / Q000201:enzymology; D007120:Immunochemistry; D008826:Microbial Sensitivity Tests; D018440:beta-Lactam Resistance; D001618:beta-Lactamases / Q000032:analysis*
chemical_list: D001426:Bacterial Proteins; D015780:Carbapenems; D003470:Culture Media; D029968:Escherichia coli Proteins; D001618:beta-Lactamases; C000601047:beta-lactamase OXA-48, E coli; C584658:beta-lactamase OXA-48, Enterobacter cloacae; C063912:carbapenemase; C108449:oxacillinase