title: Structural-based designed modular capsomere comprising HA1 for low-cost poultry influenza vaccination.
abstract: Highly pathogenic avian influenza (HPAI) viruses cause a severe and lethal infection in domestic birds. The increasing number of HPAI outbreaks has demonstrated the lack of capabilities to control the rapid spread of avian influenza. Poultry vaccination has been shown to not only reduce the virus spread in animals but also reduce the virus transmission to humans, preventing potential pandemic development. However, existing vaccine technologies cannot respond to a new virus outbreak rapidly and at a cost and scale that is commercially viable for poultry vaccination. Here, we developed modular capsomere, subunits of virus-like particle, as a low-cost poultry influenza vaccine. Modified murine polyomavirus (MuPyV) VP1 capsomere was used to present structural-based influenza Hemagglutinin (HA1) antigen. Six constructs of modular capsomeres presenting three truncated versions of HA1 and two constructs of modular capsomeres presenting non-modified HA1 have been generated. These modular capsomeres were successfully produced in stable forms using Escherichia coli, without the need for protein refolding. Based on ELISA, this adjuvanted modular capsomere (CaptHA1-3C) induced strong antibody response (almost 105endpoint titre) when administered into chickens, similar to titres obtained in the group administered with insect cell-based HA1 proteins. Chickens that received adjuvanted CaptHA1-3C followed by challenge with HPAI virus were fully protected. The results presented here indicate that this platform for bacterially-produced modular capsomere could potentially translate into a rapid-response and low-cost vaccine manufacturing technology suitable for poultry vaccination.
authors.affiliation: The University of Queensland, Australian Institute for Bioengineering and Nanotechnology, St Lucia, QLD 4072, Australia.
authors.forename: Jarurin
authors.initials: J
authors.lastname: Waneesorn
authors.affiliation: The University of Queensland, Australian Institute for Bioengineering and Nanotechnology, St Lucia, QLD 4072, Australia.
authors.forename: Nani
authors.initials: N
authors.lastname: Wibowo
authors.affiliation: Commonwealth Scientific and Industrial Research Organisation (CSIRO)-Australian Animal Health Laboratory, Geelong, VIC 3219, Australia.
authors.forename: John
authors.initials: J
authors.lastname: Bingham
authors.affiliation: The University of Queensland, Australian Institute for Bioengineering and Nanotechnology, St Lucia, QLD 4072, Australia.
authors.forename: Anton P J
authors.initials: APJ
authors.lastname: Middelberg
authors.affiliation: The University of Queensland, Protein Expression Facility, St Lucia, QLD 4072, Australia. Electronic address: l.lua@uq.edu.au.
authors.forename: Linda H L
authors.initials: LHL
authors.lastname: Lua
mesh_terms: D000276:Adjuvants, Immunologic / Q000008:administration & dosage; D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D017951:Antigen Presentation; D002645:Chickens / Q000276:immunology; D019267:Hemagglutinin Glycoproteins, Influenza Virus / Q000235:genetics / Q000276:immunology*; D006801:Humans; D007252:Influenza Vaccines / Q000191:economics / Q000235:genetics / Q000276:immunology*; D005585:Influenza in Birds / Q000517:prevention & control*; D011120:Polyomavirus; D011200:Poultry; D014611:Vaccination; D058425:Vaccines, Virus-Like Particle / Q000191:economics / Q000235:genetics / Q000276:immunology*
chemical_list: D000276:Adjuvants, Immunologic; D000914:Antibodies, Viral; D019267:Hemagglutinin Glycoproteins, Influenza Virus; D007252:Influenza Vaccines; D058425:Vaccines, Virus-Like Particle
keywords: Capsomere; Influenza; Polyomavirus; Poultry; Vaccine