title: Neuroprotective Effects of an Erythropoietin-Derived Peptide in PC1 2 Cells under Oxidative Stress.
abstract: Erythropoietin (EPO) has been shown to be a key cytokine in the production of erythrocytes from erythroblasts. Recently, attempts have been made to adopt EPO as a drug target for neuroprotection in selected neurological pathologies. In the current study, a novel EPO-derived peptide which mimics the weak binding site of EPO to its receptor (MK-X) was generated. Experimental results demonstrated that MK-X was able to ameliorate neuronal death due to reactive oxygen species and conditions of oxidative stress similar to EPO. In addition, MK-X induced long-lasting Extracellular signal-regulated protein kinases 1 and 2 (ERK1/2) and Akt activation. Furthermore, treatment with inhibitors of ERK1/2 and Akt abolished the neuroprotective effect of MK-X. Unlike EPO, however, MK-X did not induce cellular proliferation. Collectively, the results of the current study suggested that MK-X may be useful as a novel neuroprotective reagent.
authors.forename: Seung-Jun
authors.initials: SJ
authors.lastname: Yoo
authors.forename: Bongki
authors.initials: B
authors.lastname: Cho
authors.forename: Chanil
authors.initials: C
authors.lastname: Moon
authors.forename: Seong-Woon
authors.initials: SW
authors.lastname: Yu
authors.affiliation: Department of Brain and Cognitive Sciences, Graduate School, Daegu Gyeongbuk Institute of Science & Technology, 333, Techno Jungang-daero, Hyeonpung-myeon, Dalseong-gun, Daegu, Republic of Korea. cmoon@dgist.ac.kr.
authors.forename: Cheil
authors.initials: C
authors.lastname: Moon
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D016923:Cell Death / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004921:Erythropoietin / Q000737:chemistry / Q000494:pharmacology*; D006861:Hydrogen Peroxide / Q000494:pharmacology; D020935:MAP Kinase Signaling System / Q000187:drug effects; D008958:Models, Molecular; D018696:Neuroprotective Agents / Q000494:pharmacology*; D016877:Oxidants / Q000494:pharmacology; D018384:Oxidative Stress / Q000187:drug effects*; D016716:PC12 Cells / Q000187:drug effects; D010455:Peptides / Q000494:pharmacology; D011485:Protein Binding / Q000187:drug effects; D051381:Rats; D017467:Receptors, Erythropoietin / Q000378:metabolism*; D020349:Surface Plasmon Resonance
chemical_list: D018696:Neuroprotective Agents; D016877:Oxidants; D010455:Peptides; D017467:Receptors, Erythropoietin; D004921:Erythropoietin; D006861:Hydrogen Peroxide