title: Acinetobacter larvae sp. nov., isolated from the larval gut of Omphisa fuscidentalis.
abstract: A Gram-stain-negative, non-spore-forming, non-motile and aerobic coccobacilli-shaped strain, designated BRTC-1T, was isolated from the gut of Omphisa fuscidentalis Hampson, which is a larva of a moth and was collected from Xishuangbanna Dai Autonomous Prefecture in China. The isolate was found to grow at NaCl concentrations of 0-5 % (w/v) (optimum: 0 %), 10-45 °C (optimum: 30-35 °C) and pH 5.0-9.0 (optimum: pH 6.0) on tryptic soy agar. Analysis of the 16S rRNA gene sequence indicated that the isolate belonged to the genus Acinetobacter and was most closely related to Acinetobacter rudis LMG 26107T, Acinetobacter guillouiae LMG 988T and Acinetobacter bereziniae LMG 1003T with 96.4, 96.3 and 96.3 % sequence similarity, respectively. The comparative sequence analyses of the rpoB and gyrB genes showed that BRTC-1T is distant from the species of the genus Acinetobacter with validly published names (≤84.0 and ≤82.0 % similarity, respectively). The average nucleotide identity and digital DNA-DNA hybridization values (≤77.0 and ≤22.8 %, respectively) between the whole-genome sequence of BRTC-1T and those of the known taxa were far below the thresholds used to discriminate bacterial species. The major fatty acids were determined to be C16 : 0, C18 : 1ω9c and C16 : 1ω7c/iso-C15 : 0 2-OH. The respiratory quinone was Q-9. The polar lipids were found to be diphosphatidyglycerol, phosphatidylglycerol, phosphatidylethanolamine, five phospholipids and one phosphatidylcholine. Based on its phenotypic and chemotaxonomic characteristics from this study, the isolate is concluded to represent a novel species of the genus Acinetobacter, for which the name Acinetobacter larvae sp. nov. is proposed. The type strain is BRTC-1T (=ACCC 19936T=JCM 31367T).
authors.affiliation: Key Laboratory of Development and Application of Rural Renewable Energy (Ministry of Agriculture, China), Biogas Institute of Ministry of Agriculture, Chengdu 610041, PR China.
authors.forename: Song
authors.initials: S
authors.lastname: Liu
authors.affiliation: Key Laboratory of Development and Application of Rural Renewable Energy (Ministry of Agriculture, China), Biogas Institute of Ministry of Agriculture, Chengdu 610041, PR China.
authors.forename: Yanwei
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Key Laboratory of Microbial Resources (Ministry of Agriculture, China), Institute of Agricultural Resources and Regional Planning, CAAS, Beijing 100081, PR China.
authors.forename: Zhiyong
authors.initials: Z
authors.lastname: Ruan
authors.affiliation: College of Environmental and Chemical Engineering, Dalian University, Dalian 116622, PR China.
authors.forename: Kedong
authors.initials: K
authors.lastname: Ma
authors.affiliation: Key Laboratory of Development and Application of Rural Renewable Energy (Ministry of Agriculture, China), Biogas Institute of Ministry of Agriculture, Chengdu 610041, PR China.
authors.forename: Bo
authors.initials: B
authors.lastname: Wu
authors.affiliation: Key Laboratory of Development and Application of Rural Renewable Energy (Ministry of Agriculture, China), Biogas Institute of Ministry of Agriculture, Chengdu 610041, PR China.
authors.forename: Yansheng
authors.initials: Y
authors.lastname: Xu
authors.affiliation: Key Laboratory of Development and Application of Rural Renewable Energy (Ministry of Agriculture, China), Biogas Institute of Ministry of Agriculture, Chengdu 610041, PR China.
authors.forename: Jingli
authors.initials: J
authors.lastname: Wang
authors.affiliation: Key Laboratory of Development and Application of Rural Renewable Energy (Ministry of Agriculture, China), Biogas Institute of Ministry of Agriculture, Chengdu 610041, PR China.
authors.forename: Yang
authors.initials: Y
authors.lastname: You
authors.affiliation: Key Laboratory of Development and Application of Rural Renewable Energy (Ministry of Agriculture, China), Biogas Institute of Ministry of Agriculture, Chengdu 610041, PR China.
authors.forename: Mingxiong
authors.initials: M
authors.lastname: He
authors.affiliation: Key Laboratory of Development and Application of Rural Renewable Energy (Ministry of Agriculture, China), Biogas Institute of Ministry of Agriculture, Chengdu 610041, PR China.
authors.forename: Guoquan
authors.initials: G
authors.lastname: Hu
mesh_terms: D000150:Acinetobacter / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D000818:Animals; D015373:Bacterial Typing Techniques; D001482:Base Composition; D002681:China; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D005798:Genes, Bacterial; D007814:Larva / Q000382:microbiology; D009036:Moths / Q000382:microbiology*; D009693:Nucleic Acid Hybridization; D010743:Phospholipids / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; C073355:menaquinone 9