title: ACE Inhibitory and Antioxidant Activities of Collagen Hydrolysates from the Ribbon Jellyfish (Chrysaorasp.).
abstract: Collagen isolated from the ribbon jellyfish (Chrysaorasp.) was hydrolysed using three different proteases (i.e.trypsin, alcalase and Protamex) to obtain bioactive peptides. Angiotensin-I-converting enzyme (ACE) inhibitory activity and antioxidant activities (i.e.ferric reducing antioxidant power (FRAP) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity) of the peptides were measured and compared, and the effect of the duration of hydrolysis on the bioactivity (ACE inhibitory and antioxidant activities) of peptides was also evaluated. FRAP activity was the highest in Protamex-induced (25-27 mM) and trypsin-induced hydrolysates (24-26 mM) at 7 and 9 h, respectively. Conversely, hydrolysates produced by trypsin for 1 and 3 h showed the highest DPPH radical scavenging activities (94 and 92%, respectively). Trypsin-induced hydrolysates (at 3 h) also showed the highest ACE inhibitory activity (89%). The peptide sequences with the highest activities were identified using tandem mass spectrometry, and the results show that the hydrolysates had a high content of hydrophobic amino acids as well as unique amino acid sequences, which likely contribute to their biological activities.
authors.affiliation: Food Technology Division, School of Industrial Technology, Universiti Sains Malaysia, 11800 Minden, Penang, Malaysia; Centre for Advanced Analytical Toxicology Services, Universiti Sains Malaysia, 11800 Minden, Penang, Malaysia.
authors.forename: Zoha
authors.initials: Z
authors.lastname: Barzideh
authors.affiliation: Toxicology and Multipurpose Laboratory, Anti Doping Laboratory Qatar, Doha, Qatar.
authors.forename: Aishah Abd
authors.initials: AA
authors.lastname: Latiff
authors.affiliation: Centre for Advanced Analytical Toxicology Services, Universiti Sains Malaysia, 11800 Minden, Penang, Malaysia.
authors.forename: Chee-Yuen
authors.initials: CY
authors.lastname: Gan
authors.affiliation: Food Technology Division, School of Industrial Technology, Universiti Sains Malaysia, 11800 Minden, Penang, Malaysia.
authors.forename: Md Zainul
authors.initials: MZ
authors.lastname: Abedin
authors.affiliation: Food Technology Division, School of Industrial Technology, Universiti Sains Malaysia, 11800 Minden, Penang, Malaysia.
authors.forename: Abd Karim
authors.initials: AK
authors.lastname: Alias
keywords: ACE inhibition; Chrysaora sp.; DPPH; FRAP; antihypertensive activity; antioxidant activity; collagen; jellyfish; protein hydrolysates