title: The change of HCN1/HCN2 mRNA expression in peripheral nerve after chronic constriction injury induced neuropathy followed by pulsed electromagnetic field therapy.
abstract: Neuropathic pain is usually defined as a chronic pain state caused by peripheral or central nerve injury as a result of acute damage or systemic diseases. It remains a difficult disease to treat. Recent studies showed that the frequency of action potentials in nociceptive afferents is affected by the activity of hyperpolarization-activated cyclic nucleotide-gated cation channels (HCN) family. In the current study, we used a neuropathy rat model induced by chronic constriction injury (CCI) of sciatic nerve to evaluate the change of expression of HCN1/HCN2 mRNA in peripheral nerve and spinal cord. Rats were subjected to CCI with or without pulsed electromagnetic field (PEMF) therapy. It was found that CCI induced neural cell degeneration while PEMF promoted nerve regeneration as documented by Nissl staining. CCI shortened the hind paw withdrawal latency (PWL) and hind paw withdrawal threshold (PWT) and PEMF prolonged the PWL and PWT. In addition, CCI lowers the expression of HCN1 and HCN2 mRNA and PEMF cannot restore the expression of HCN1 and HCN2 mRNA. Our results indicated that PEMF can promote nerve regeneration and could be used for the treatment of neuropathic pain.
authors.affiliation: Department of Anesthesiology, Jiangsu Cancer Hospital, Jiangsu 210009, China.|Department of Anesthesiology, West China Hospital, Sichuan University, Sichuan 610041, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Liu
authors.affiliation: Department of Rehabilitation, West China Hospital, Sichuan University, Sichuan 610041, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Zhou
authors.affiliation: Department of Anesthesiology, Jiangsu Cancer Hospital, Jiangsu 210009, China.
authors.forename: Lianbing
authors.initials: L
authors.lastname: Gu
authors.affiliation: Department of Anesthesiology, West China Hospital, Sichuan University, Sichuan 610041, China.
authors.forename: Yunxia
authors.initials: Y
authors.lastname: Zuo
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D005786:Gene Expression Regulation; D064428:Hyperpolarization-Activated Cyclic Nucleotide-Gated Channels / Q000235:genetics*; D055909:Magnetic Field Therapy / Q000379:methods; D008297:Male; D009410:Nerve Degeneration; D009416:Nerve Regeneration; D059348:Peripheral Nerve Injuries / Q000209:etiology* / Q000473:pathology* / Q000628:therapy; D015221:Potassium Channels / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics*; D051381:Rats
chemical_list: C578667:Hcn1 protein, rat; C578712:Hcn2 protein, rat; D064428:Hyperpolarization-Activated Cyclic Nucleotide-Gated Channels; D015221:Potassium Channels; D012333:RNA, Messenger
keywords: chronic constriction injury (CCI); hyperpolarization-activated cyclic nucleotide-gated cation channels (HCN); pulsed electromagnetic field (PEMF)