title: Effect of Cardiac Myosin-Binding Protein C on Tropomyosin Regulation of Actin-Myosin Interaction Using In Vitro Motility Assay.
abstract: We studied the modulating role of cardiac myosin-binding protein C (cMyBP-C) in tropomyosin regulation of the actin-myosin interaction. The effect of cMyBP-C on the velocity of actin-tropomyosin filament sliding over cardiac and slow skeletal myosins was evaluated using in vitro motility assay. The effect of cMyBP-C on the actin-tropomyosin filaments sliding depended on the type of myosin. The regulatory effect of cMyBP-C differs for cardiac and slow skeletal myosin because of the presence of specific essential light chain (LC1sa) in slow skeletal myosin isoform.
authors.affiliation: Institute of Immunology and Physiology, Ural Division of the Russian Academy of Sciences, Ekaterinburg, Russia.
authors.forename: D V
authors.initials: DV
authors.lastname: Shchepkin
authors.affiliation: Institute of Immunology and Physiology, Ural Division of the Russian Academy of Sciences, Ekaterinburg, Russia.
authors.forename: G V
authors.initials: GV
authors.lastname: Kopylova
authors.affiliation: Institute of Immunology and Physiology, Ural Division of the Russian Academy of Sciences, Ekaterinburg, Russia. l.nikitina@iip.uran.ru.
authors.forename: L V
authors.initials: LV
authors.lastname: Nikitina
mesh_terms: D000199:Actins / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D000818:Animals; D001681:Biological Assay; D002021:Buffers; D002352:Carrier Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D002417:Cattle; D002645:Chickens; D015870:Gene Expression; D006801:Humans; D009038:Motion; D018482:Muscle, Skeletal / Q000737:chemistry / Q000502:physiology; D009206:Myocardium / Q000737:chemistry / Q000378:metabolism; D009218:Myosins / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D009928:Organ Specificity; D011485:Protein Binding; D020033:Protein Isoforms / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D011817:Rabbits; D012996:Solutions; D014335:Tropomyosin / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D000099163:Myosin Binding Protein C
chemical_list: D000199:Actins; D002021:Buffers; D002352:Carrier Proteins; D009218:Myosins; D020033:Protein Isoforms; D012996:Solutions; D014335:Tropomyosin; D000099163:Myosin Binding Protein C
keywords: cardiac myosin-binding protein C; myosin; tropomyosin