title: Retinoid acid-induced microRNA-27b-3p impairs C2C12 myoblast proliferation and differentiation by suppressing α-dystrobrevin.
abstract: We previously reported that excess retinoic acid (RA) resulted in hypoplastic and derangement of myofilaments in embryonic tongue by inhibiting myogenic proliferation and differentiation through CamKIID pathway. Our further studies revealed that the expression of a series of miRNAs was altered by RA administration in embryonic tongue as well as in C2C12 cells. Thus, if excess RA impairs myogenic proliferation and differentiation through miRNAs is taken into account. In present study, miR-27b-3p was found up-regulated in RA-treated C2C12 cells as in embryonic tongue, and predicted to target the 3'UTR of α-dystrobrevin (DTNA). Luciferase reporter assays confirmed the direct interaction between miR-27b-3p and the 3'UTR of DTNA. MiR-27b-3p mimics recapitulated the RA repression on DTNA expression, C2C12 proliferation and differentiation, while the miR-27b-3p inhibitor circumvented these defects resulting from excess RA. As expected, the effects of siDTNA on C2C12 were coincided with those by RA treatment or miR-27b-3p mimics. Therefore, these findings indicated that excess RA inhibited the myoblast proliferation and differentiation by up-regulating miR-27b-3p to target DTNA, which implied a new mechanism in myogenic hypoplasia.
authors.affiliation: Department of Oral Pathology, College of Stomatology, Dalian Medical University, Dalian, Liaoning 116044, China.
authors.forename: Nan
authors.initials: N
authors.lastname: Li
authors.affiliation: Department of Oral Pathology, College of Stomatology, Dalian Medical University, Dalian, Liaoning 116044, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Tang
authors.affiliation: Department of Oral Pathology, College of Stomatology, Dalian Medical University, Dalian, Liaoning 116044, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Liu
authors.affiliation: Department of Oral Pathology, College of Stomatology, Dalian Medical University, Dalian, Liaoning 116044, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Cong
authors.affiliation: Department of Oral Pathology, College of Stomatology, Dalian Medical University, Dalian, Liaoning 116044, China. Electronic address: liuchao_19760711@yahoo.com.
authors.forename: Chao
authors.initials: C
authors.lastname: Liu
authors.affiliation: Department of Oral Pathology, College of Stomatology, Dalian Medical University, Dalian, Liaoning 116044, China. Electronic address: xiaoj@dmu.edu.cn.
authors.forename: Jing
authors.initials: J
authors.lastname: Xiao
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D002460:Cell Line; D049109:Cell Proliferation; D049029:Dystrophin-Associated Proteins / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D035683:MicroRNAs / Q000235:genetics*; D024510:Muscle Development; D032446:Myoblasts / Q000166:cytology / Q000187:drug effects / Q000378:metabolism* / Q000502:physiology; D014059:Tongue / Q000187:drug effects / Q000196:embryology / Q000378:metabolism; D014212:Tretinoin / Q000494:pharmacology
chemical_list: D049029:Dystrophin-Associated Proteins; D035683:MicroRNAs; C540674:Mirn27 microRNA, mouse; C098885:dystrobrevin; D014212:Tretinoin
keywords: MicroRNA; Myogenesis; Retinoid acid; Tongue; α-dystrobrevin