title: Salvianolic Acid B Alleviates Heart Failure by Inactivating ERK1/2/GATA4 Signaling Pathway after Pressure Overload in Mice.
abstract: BACKGROUND
  Heart failure(HF) is a dangerous disease that affects millions of patients. Radix Salvia is widely used in Chinese clinics to treat heart diseases. Salvianolic acid B(SalB) is the major active component of Radix Salvia. This study investigated the mechanisms of action and effects of SalB on HF in an experimental mouse model of HF.
  
  
  METHODS
  We created a mouse model of HF by inducing pressure overload with transverse aortic constriction(TAC) surgery for 2 weeks and compared among 4 study groups: SHAM group (n = 10), TAC group (n = 9), TAC+MET group (metprolol, positive drug treatment, n = 9) and TAC+SalB group (SalB, 240 mg•kg-1•day-1, n = 9). Echocardiography was used to evaluate the dynamic changes in cardiac structure and function in vivo. Plasma brain natriuretic peptide (BNP) concentration was detected by Elisa method. In addition, H9C2 rat cardiomyocytes were cultured and Western blot were implemented to evaluate the phosphorylation of ERK1/2, AKT, and protein expression of GATA4.
  
  
  RESULTS
  SalB significantly inhibited the phosphorylation of Thr202/Tyr204 sites of ERK1/2, but not Ser473 site of AKT, subsequently inhibited protein expression of GATA4 and plasma BNP(P < 0.001), and then inhibited HF at 2 weeks after TAC surgery.
  
  
  CONCLUSIONS
  Our data provide a mechanism of inactivating the ERK1/2/GATA4 signaling pathway for SalB inhibition of the TAC-induced HF.
authors.affiliation: Laboratory Animal Center, Southern Medical University, Guangzhou city, Guangdong province, China.|Animal Laboratory, Guangdong Province Academy of Chinese Medicine, Guangzhou city, Guangdong province, China.
authors.forename: Juan
authors.initials: J
authors.lastname: Yu
authors.affiliation: AMI Key Laboratory of Chinese Medicine in Guangzhou, Guangdong Province Academy of Chinese Medicine, Guangdong Province Hospital of Chinese Medicine, 2nd Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou city,Guangdong province, China.
authors.forename: Renshan
authors.initials: R
authors.lastname: Chen
authors.affiliation: AMI Key Laboratory of Chinese Medicine in Guangzhou, Guangdong Province Academy of Chinese Medicine, Guangdong Province Hospital of Chinese Medicine, 2nd Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou city,Guangdong province, China.
authors.forename: Yafang
authors.initials: Y
authors.lastname: Tan
authors.affiliation: Department of Pharmaceutical Sciences, College of Pharmacy, Northeast Ohio Medical University, Rootstown, Ohio, Unitd States of America.
authors.forename: Jiashin
authors.initials: J
authors.lastname: Wu
authors.affiliation: AMI Key Laboratory of Chinese Medicine in Guangzhou, Guangdong Province Academy of Chinese Medicine, Guangdong Province Hospital of Chinese Medicine, 2nd Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou city,Guangdong province, China.
authors.forename: Jianyong
authors.identifier: http://orcid.org/0000-0002-2290-740X
authors.initials: J
authors.lastname: Qi
authors.affiliation: AMI Key Laboratory of Chinese Medicine in Guangzhou, Guangdong Province Academy of Chinese Medicine, Guangdong Province Hospital of Chinese Medicine, 2nd Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou city,Guangdong province, China.
authors.forename: Minzhou
authors.initials: M
authors.lastname: Zhang
authors.affiliation: Laboratory Animal Center, Southern Medical University, Guangzhou city, Guangdong province, China.
authors.forename: Weiwang
authors.initials: W
authors.lastname: Gu
mesh_terms: D000818:Animals; D001013:Aorta, Thoracic / Q000601:surgery; D001572:Benzofurans / Q000737:chemistry / Q000494:pharmacology*; D001794:Blood Pressure / Q000187:drug effects; D002460:Cell Line; D004195:Disease Models, Animal; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000494:pharmacology; D050991:GATA4 Transcription Factor / Q000378:metabolism; D006333:Heart Failure / Q000378:metabolism / Q000473:pathology*; D006352:Heart Ventricles / Q000000981:diagnostic imaging; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D019950:Mitogen-Activated Protein Kinase 1 / Q000378:metabolism; D048052:Mitogen-Activated Protein Kinase 3 / Q000378:metabolism; D009206:Myocardium / Q000378:metabolism / Q000473:pathology; D032383:Myocytes, Cardiac / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D020097:Natriuretic Peptide, Brain / Q000097:blood; D010766:Phosphorylation / Q000187:drug effects; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism; D051381:Rats; D015398:Signal Transduction / Q000187:drug effects*; D053630:Depsides
chemical_list: D001572:Benzofurans; D004365:Drugs, Chinese Herbal; D050991:GATA4 Transcription Factor; D019950:Mitogen-Activated Protein Kinase 1; D048052:Mitogen-Activated Protein Kinase 3; D020097:Natriuretic Peptide, Brain; D051057:Proto-Oncogene Proteins c-akt; C484171:Gata4 protein, mouse; C076944:salvianolic acid B; D053630:Depsides