title: Evaluation of synovium-derived mesenchymal stem cells and 3D printed nanocomposite scaffolds for tissue engineering.
abstract: Stem cells and scaffolds play a very important role in tissue engineering. Here, we isolated synovium-derived mesenchymal stem cells (SMSCs) from synovial membrane tissue and characterized stem-cell properties. Gelatin nanoparticles (NP) were prepared using a two-step desolvation method and then pre-mixed into different host matrix (silk fibroin (SF), gelatin (Gel), or SF-Gel mixture) to generate various 3D printed nanocomposite scaffolds (NP/SF, NP/SF-Gel, NP/Gel-1, and NP/Gel-2). The microstructure was examined by scanning electron microscopy. Biocompatibility assessment was performed through CCK-8 assay by coculturing with SMSCs at 1, 3, 7 and 14 days. According to the results, SMSCs are similar to other MSCs in their surface epitope expression, which are negative for CD45 and positive for CD44, CD90, and CD105. After incubation in lineage-specific medium, SMSCs could differentiate into chondrocytes, osteocytes and adipocytes. 3D printed nanocomposite scaffolds exhibited a good biocompatibility in the process of coculturing with SMSCs and had no negative effect on cell behavior. The study provides a strategy to obtain SMSCs and fabricate 3D printed nanocomposite scaffolds, the combination of which could be used for practical applications in tissue engineering.
authors.affiliation: Department of Orthopedics, Zhongshan Hospital of Fudan University, 180 Fenglin Road, Shanghai 200032, People's Republic of China.
authors.forename: Jian-Feng
authors.initials: JF
authors.lastname: Pan
authors.affiliation: Department of Orthopedics, Zhongshan Hospital of Fudan University, 180 Fenglin Road, Shanghai 200032, People's Republic of China.
authors.forename: Shuo
authors.initials: S
authors.lastname: Li
authors.affiliation: Department of Orthopedics, Zhongshan Hospital of Fudan University, 180 Fenglin Road, Shanghai 200032, People's Republic of China.
authors.forename: Chang-An
authors.initials: CA
authors.lastname: Guo
authors.affiliation: State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, College of Materials Science and Engineering, Donghua University, 2999 North Renmin Road, Shanghai 201620, People's Republic of China; Biomaterials and Tissue Engineering Lab, College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, 2999 North Renmin Road, Shanghai 201620, People's Republic of China.
authors.forename: Du-Liang
authors.initials: DL
authors.lastname: Xu
authors.affiliation: Department of Orthopedics, Zhongshan Hospital of Fudan University, 180 Fenglin Road, Shanghai 200032, People's Republic of China.
authors.forename: Feng
authors.initials: F
authors.lastname: Zhang
authors.affiliation: Department of Orthopedics, Zhongshan Hospital of Fudan University, 180 Fenglin Road, Shanghai 200032, People's Republic of China.
authors.forename: Zuo-Qin
authors.initials: ZQ
authors.lastname: Yan
authors.affiliation: State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, College of Materials Science and Engineering, Donghua University, 2999 North Renmin Road, Shanghai 201620, People's Republic of China; Biomaterials and Tissue Engineering Lab, College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, 2999 North Renmin Road, Shanghai 201620, People's Republic of China.
authors.forename: Xiu-Mei
authors.initials: XM
authors.lastname: Mo
keywords: 3D printed nanocomposite scaffolds; nanoparticles; synovium-derived mesenchymal stem cells