title: Histologic Evidence of New Collagen Formulation Using Platelet Rich Plasma in Skin Rejuvenation: A Prospective Controlled Clinical Study.
abstract: BACKGROUND
  Platelet-rich plasma (PRP) is an autologous concentration of human platelets contained in a small volume of plasma and has recently been shown to accelerate rejuvenate aging skin by various growth factors and cell adhesion molecules.
  
  
  OBJECTIVE
  This study was conducted to evaluate the efficacy and safety of intradermal injection of PRP in the human facial rejuvenation.
  
  
  METHODS
  This study was a prospective, single-center, single-dose, open-label, non-randomized controlled clinical study. PRP injected to the upper site of this right infra-auricular area and all face. Saline was injected to the left infra-auricular area. Histopathological examinations were performed before PRP treatment, 28 days after the PRP, and saline (control) treatments.
  
  
  RESULTS
  Twenty women ranging in age from 40 to 49 years (mean age, 43.65±2.43 years) were enrolled in the study. The mean optical densities (MODs) of collagen in the pre-treatment, control, and PRP-treated area were measured. They were 539±93.2, 787±134.15, 1,019±178, respectively. In the MOD of PRP, 89.05 percent improvement was found when MOD of PRP was compared with MOD of pre-treatment. The mean MOD of collagen fibers was clearly highest on the PRP side (p<0.001). The PRP-to-saline improvement ratio (89.05% to 46.01%) was 1.93:1. No serious side effects were detected.
  
  
  CONCLUSIONS
  PRP increases dermal collagen levels not only by growth factors, but also by skin needling (the mesotherapy technique 'point by point'). PRP application could be considered as an effective (even a single application) and safety procedure for facial skin rejuvenation.
authors.affiliation: Department of Dermatology, Acıbadem Fulya Hospital, Istanbul, Turkey.
authors.forename: Ozlem Karabudak
authors.initials: OK
authors.lastname: Abuaf
authors.affiliation: Department of Dermatology, Eskisehir Military Hospital, Eskisehir, Turkey.
authors.forename: Hamza
authors.initials: H
authors.lastname: Yildiz
authors.affiliation: Department of Pathology, Anadolu Medical Center, Kocaeli, Turkey.
authors.forename: Hüseyin
authors.initials: H
authors.lastname: Baloglu
authors.affiliation: Department of Dermatology, Yunus Emre Government Hospital, Eskisehir, Turkey.
authors.forename: Memet Ersan
authors.initials: ME
authors.lastname: Bilgili
authors.affiliation: Department of Pathology, Eskisehir Military Hospital, Eskisehir, Turkey.
authors.forename: Hasan Aktug
authors.initials: HA
authors.lastname: Simsek
authors.affiliation: Department of Dermatology, GATA Haydarpasa Teaching Hospital, Istanbul, Turkey.
authors.forename: Bilal
authors.initials: B
authors.lastname: Dogan
keywords: Collagen; Mesotherapy; Platelet-rich plasma; Skin needling; Skin rejuvenation