title: Methods for Proteome Analysis of Campylobacter jejuni Using 2-D Electrophoresis.
abstract: This chapter describes protocols used for two-dimensional electrophoretic analysis of the proteome or subproteome of Campylobacter jejuni, a major human food-borne pathogen. The following protocols, adapted to Campylobacter strains, include all the steps from cultivation to gel-support protein separation.
authors.affiliation: INRA, UMR 1014 Secalim, Nantes, F-44307, France.|LUNAM Université Oniris, Nantes, F-44307, France.
authors.forename: Ramila C
authors.initials: RC
authors.lastname: Rodrigues
authors.affiliation: INRA, UMR 1014 Secalim, Nantes, F-44307, France.|LUNAM Université Oniris, Nantes, F-44307, France.
authors.forename: Nabila
authors.initials: N
authors.lastname: Haddad
authors.affiliation: INRA, UMR 1014 Secalim, Nantes, F-44307, France. odile.tresse@oniris-nantes.fr.|LUNAM Université Oniris, Nantes, F-44307, France. odile.tresse@oniris-nantes.fr.
authors.forename: Odile
authors.initials: O
authors.lastname: Tresse
mesh_terms: D001426:Bacterial Proteins / Q000145:classification / Q000302:isolation & purification* / Q000378:metabolism; D016123:Campylobacter jejuni / Q000737:chemistry* / Q000254:growth & development / Q000378:metabolism; D003470:Culture Media / Q000737:chemistry; D003720:Densitometry; D003956:Dialysis; D015180:Electrophoresis, Gel, Two-Dimensional / Q000295:instrumentation / Q000379:methods*; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D007525:Isoelectric Focusing / Q000379:methods*; D007526:Isoelectric Point; D020543:Proteome / Q000302:isolation & purification* / Q000378:metabolism; D016622:Silver Staining
chemical_list: D001426:Bacterial Proteins; D003470:Culture Media; D020543:Proteome
keywords: 2-D electrophoresis; Campylobacter; Isoelectric point; Protein extraction; Protein purification; SDS-PAGE