title: Genotyping of German and Austrian Taylorella equigenitalis isolates using repetitive extragenic palindromic (REP) PCR and pulsed-field gel electrophoresis (PFGE).
abstract: A total of 124 Taylorella (T.) equigenitalis and five T. asinigenitalis field isolates collected between 2002 and 2014 were available for genotyping using REP- (repetitive extragenic palindromic) PCR and PFGE (pulsed-field gel electrophoresis). The study comprised 79 T. equigenitalis field isolates originating from ten defined breeds of German horses and revealed a spectrum of five REP (rep-E1-E4, rep-E3a) and 15 PFGE (TE-A1-A9, TE-B1-B3, TE-C, TE-E1, and TE-E2) genotypes. T. equigenitalis field isolates (n=40) obtained from Austrian Lipizzaner horses were differentiated into three REP (rep-E1, rep-E3a, and rep-E4) and three PFGE genotypes (TE-A2, TE-A5, and TE-D); those isolated from four Austrian Trotters belonged to the REP/PFGE genotype rep-E2/TE-A1. Interestingly, a T. equigenitalis isolate recovered from a Holsteiner stallion living in South Africa revealed the REP/PFGE genotype rep-E1/TE-A5 which was otherwise exclusively present in the majority of Austrian Lipizzaner horses in our study. The type strain included in this study revealed the genotype REP/PFGE rep-E1/TE-F. Six strains of T. asinigenitalis including the type strain were separated into three REP (rep-A1-A3) and six PFGE genotypes (TA-A1, TA-A2, TA-A3, TA-B, TA-C, TA-D). Overall, the generated REP and PFGE genotypes showed a good correlation, whereas REP-PCR proved to be a suitable method for molecular epidemiological screening of T. equigenitalis and T. asinigenitalis isolates that should be differentiated in detail by genotyping using PFGE.
authors.affiliation: Chemisches und Veterinäruntersuchungsamt Stuttgart (Chemical and Veterinary Investigations Office Stuttgart), Schaflandstr. 3/3, Fellbach D-70736, Germany. Electronic address: reinhard.sting@cvuas.bwl.de.
authors.forename: Reinhard
authors.initials: R
authors.lastname: Sting
authors.affiliation: Pferdegesundheitsdienst Stuttgart, Tierseuchenkasse Baden-Württemberg (Animal Health Service Stuttgart, Animal Diseases Fund Baden-Wuerttemberg), Schaflandstr. 3/3, Fellbach D-70736, Germany.
authors.forename: Christoph
authors.initials: C
authors.lastname: Seeh
authors.affiliation: Chemisches und Veterinäruntersuchungsamt Stuttgart (Chemical and Veterinary Investigations Office Stuttgart), Schaflandstr. 3/3, Fellbach D-70736, Germany.
authors.forename: Norman
authors.initials: N
authors.lastname: Mauder
authors.affiliation: Institut für Mikrobiologie, Veterinärmedizinische Universität Wien (Institute of Microbiology, University of Veterinary Medicine Vienna), Veterinärplatz 1, A-1210 Vienna, Austria.
authors.forename: Michaela
authors.initials: M
authors.lastname: Maurer
authors.affiliation: Institut für Mikrobiologie, Veterinärmedizinische Universität Wien (Institute of Microbiology, University of Veterinary Medicine Vienna), Veterinärplatz 1, A-1210 Vienna, Austria.
authors.forename: Igor
authors.initials: I
authors.lastname: Loncaric
authors.affiliation: Institut für Milchhygiene, Veterinärmedizinische Universität Wien (Institute for Milk Hygiene, Milk Technology and Food Science, University of Veterinary Medicine Vienna), Veterinärplatz 1, A-1210 Vienna, Austria.
authors.forename: Beatrix
authors.initials: B
authors.lastname: Stessl
authors.affiliation: IDEXX Vet·Med·Labor, Mörikestr. 28/3, D-71636, Ludwigsburg, Germany.
authors.forename: Peter
authors.initials: P
authors.lastname: Kopp
authors.affiliation: Pferdegesundheitsdienst Aulendorf, Tierseuchenkasse Baden-Württemberg (Animal Health Service Aulendorf, Animal Diseases Fund Baden-Wuerttemberg), Talstr. 17, D-88326, Aulendorf, Germany.
authors.forename: Klaus
authors.initials: K
authors.lastname: Banzhaf
authors.affiliation: Staatliches Tierärztliches Untersuchungsamt Aulendorf - Diagnostikzentrum (Aulendorf State Veterinary Diagnostic Centre), Löwenbreitestr. 18/20, D-88326, Aulendorf, Germany.
authors.forename: Brigitte
authors.initials: B
authors.lastname: Martin
authors.affiliation: Friedrich-Loeffler-Institut, Institut für bakterielle Infektionen und Zoonosen, Nationales Referenzlabor für Kontagiöse Equine Metritis (Institute of Bacterial Infections and Zoonoses, National Reference Laboratory for Contagious Equine Metritis), Naumburger Str. 96 a, D-07743, Jena, Germany.
authors.forename: Falk
authors.initials: F
authors.lastname: Melzer
authors.affiliation: Friedrich-Loeffler-Institut, Institut für bakterielle Infektionen und Zoonosen, Nationales Referenzlabor für Kontagiöse Equine Metritis (Institute of Bacterial Infections and Zoonoses, National Reference Laboratory for Contagious Equine Metritis), Naumburger Str. 96 a, D-07743, Jena, Germany.
authors.forename: Astrid
authors.initials: A
authors.lastname: Raßbach
authors.affiliation: Institut für Mikrobiologie, Veterinärmedizinische Universität Wien (Institute of Microbiology, University of Veterinary Medicine Vienna), Veterinärplatz 1, A-1210 Vienna, Austria.
authors.forename: Joachim
authors.initials: J
authors.lastname: Spergser
mesh_terms: D000818:Animals; D001317:Austria; D016521:Electrophoresis, Gel, Pulsed-Field / Q000662:veterinary; D005260:Female; D005838:Genotype; D005858:Germany; D016905:Gram-Negative Bacterial Infections / Q000235:genetics / Q000382:microbiology / Q000662:veterinary*; D006734:Horse Diseases / Q000235:genetics* / Q000382:microbiology; D006736:Horses; D055029:Inverted Repeat Sequences; D008297:Male; D016133:Polymerase Chain Reaction / Q000662:veterinary; D020625:Taylorella equigenitalis / Q000235:genetics*
keywords: Genotyping; PFGE; Rep-PCR; Taylorella asinigenitalis; Taylorella equigenitalis