title: Single-cell RNA-seq reveals lincRNA expression differences in Hela-S3 cells.
abstract: OBJECTIVE
  To characterize transcriptome-wide lincRNAs of Hela-S3 cell line by analyzing RNA sequencing data to provide a foundation for further functional verification and clinical application of cervical carcinoma development.
  
  
  RESULTS
  Single-cell RNA sequencing data of 37 Hela-S3 cells were analysed. On average, 511 lincRNAs were expressed in each cell. Comparing the expression difference of the lincRNAs and protein-coding genes, we found that lincRNAs expression displayed more cell specificity than that of protein-coding genes (t-test, P<2.2E-16). In co-expression network analysis, we identified seven modules and one of them was enriched in pathways of mitotic, packaging of telomere ends, and chromosome maintenance.
  
  
  CONCLUSIONS
  incRNAs are specifically expressed and form a network to perform function at single cell level. Their expression was more specific than that of protein-coding genes.
authors.affiliation: BGI Education Center, University of Chinese Academy of Sciences, Shenzhen, 518083, China. wangjie1@genomics.cn.|BGI-Shenzhen, Shenzhen, 518083, China. wangjie1@genomics.cn.|BGI Genomics Co. Ltd., Shenzhen, 518083, China. wangjie1@genomics.cn.
authors.forename: Jie
authors.initials: J
authors.lastname: Wang
authors.affiliation: BGI Education Center, University of Chinese Academy of Sciences, Shenzhen, 518083, China.|BGI-Shenzhen, Shenzhen, 518083, China.|BGI Genomics Co. Ltd., Shenzhen, 518083, China.
authors.forename: Bhaskar
authors.initials: B
authors.lastname: Roy
mesh_terms: D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D053263:Gene Regulatory Networks; D006367:HeLa Cells; D006801:Humans; D016366:Open Reading Frames / Q000235:genetics; D062085:RNA, Long Noncoding / Q000235:genetics* / Q000378:metabolism; D017423:Sequence Analysis, RNA / Q000379:methods*; D059010:Single-Cell Analysis / Q000379:methods*
chemical_list: D062085:RNA, Long Noncoding
keywords: Cervical carcinoma; Hela cell line; LincRNAs; RNA-seq