title: Sphingomonas lutea sp. nov., isolated from freshwater of an artificial reservoir.
abstract: An aerobic, Gram-stain-negative, gliding and yellow-pigmented bacterium, designated strain JS5T, was isolated from freshwater of Juam reservoir, Republic of Korea. Cells were catalase-positive and oxidase-negative. The neighbour-joining phylogenetic tree based on 16S rRNA gene sequences showed that strain JS5T forms an independent lineage within the genus Sphingomonas. Strain JS5T was related distantly to 'Sphingomonas parvus' GP20-2 (98.2 % 16S rRNA gene sequence similarity), Sphingomonas sediminicola Dae 20T (96.8 %) and Sphingomonas daechungensis CH15-11T (96.7 %). The major fatty acids of strain JS5T were C16 : 0, summed feature 3 comprising C16 : 1ω7c and/or C16 : 1ω6c and summed feature 8 comprising C18 : 1ω7c and/or C18 : 1ω6c. The predominant isoprenoid quinone of the isolate was ubiquinone-10. The DNA G+C content of strain JS5T was 65 mol%. Phenotypic characteristics distinguished strain JS5T from related species of the genus Sphingomonas. On the basis of the evidence presented in this study, a novel species, Sphingomonaslutea sp. nov., is proposed to accommodate strain JS5T (=KCTC 23642T=JCM 18309T).
authors.affiliation: Department of Biology, College of Life Science and Natural Resources, Sunchon National University, Suncheon 57922, Republic of Korea.
authors.forename: Ji Hee
authors.initials: JH
authors.lastname: Lee
authors.affiliation: Department of Biology, College of Life Science and Natural Resources, Sunchon National University, Suncheon 57922, Republic of Korea.
authors.forename: Dae In
authors.initials: DI
authors.lastname: Kim
authors.affiliation: Department of Biology, College of Life Science and Natural Resources, Sunchon National University, Suncheon 57922, Republic of Korea.
authors.forename: Joo Won
authors.initials: JW
authors.lastname: Kang
authors.affiliation: Department of Biology, College of Life Science and Natural Resources, Sunchon National University, Suncheon 57922, Republic of Korea.
authors.forename: Chi Nam
authors.initials: CN
authors.lastname: Seong
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D005618:Fresh Water / Q000382:microbiology*; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D010858:Pigmentation; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D056910:Republic of Korea; D017422:Sequence Analysis, DNA; D020578:Sphingomonas / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D014451:Ubiquinone / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D014451:Ubiquinone; C025204:Ubiquinone Q2