title: MiR-330-3p inhibits gastric cancer progression through targeting MSI1.
abstract: Increasing evidences demonstrated that microRNAs (miRNAs) play critical roles in the human tumor development and progression. In our study, we found that miR-330-3p expression was downregulated in gastric cancer cell lines and tissues. Ectopic expression of miR-330-3p suppressed the gastric cancer cell proliferation, colony formation and migration. Overexpression of miR-330-3p promoted E-cadherin expression and inhibited the expression of N-cadherin, vimentin and snail. We identified Musashi-1 (MSI1) as a direct target gene of miR-330-3p in gastric cancer cell. In addition, MSI1 was upregulated in gastric cancer cell lines and tissues and the MSI1 expression was inversely correlated with miR-330-3p expression in gastric cancer tissues. MiR-330-3p expression was increased in gastric cancer cells after treated with histone deacetylase inhibitor trichostatin A (TSA) and DNA methylation inhibitor 5-aza-CdR (AZA). These indicated that downregulated expression of miR-330-3p was partly mediated by gene promoter region hypermethylation. These results suggested that miR-330-3p acted as a tumor suppressor gene in GC.
authors.affiliation: Department of General Surgery, The Affiliated Yan an Hospital of Kunming Medical University Kunming 650051, Yunnan, China.
authors.forename: Aoran
authors.initials: A
authors.lastname: Guan
authors.affiliation: Department of Gastroenterology, The Affiliated Yan an Hospital of Kunming Medical University Kunming 650051, Yunnan, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Wang
authors.affiliation: Department of General Surgery, The Affiliated Yan an Hospital of Kunming Medical University Kunming 650051, Yunnan, China.
authors.forename: Xun
authors.initials: X
authors.lastname: Li
authors.affiliation: Department of General Surgery, The Affiliated Yan an Hospital of Kunming Medical University Kunming 650051, Yunnan, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Xie
authors.affiliation: Department of General Surgery, The Affiliated Yan an Hospital of Kunming Medical University Kunming 650051, Yunnan, China.
authors.forename: Ruotian
authors.initials: R
authors.lastname: Wang
authors.affiliation: Department of General Surgery, The Affiliated Yan an Hospital of Kunming Medical University Kunming 650051, Yunnan, China.
authors.forename: Yankun
authors.initials: Y
authors.lastname: Zhu
authors.affiliation: Department of General Surgery, The Affiliated Yan an Hospital of Kunming Medical University Kunming 650051, Yunnan, China.
authors.forename: Ruhong
authors.initials: R
authors.lastname: Li
keywords: Gastric cancer; Musashi-1 (MSI1); miR-330-3p; miRNAs; microRNAs