title: The role of ficolin-like protein (PcFLP1) in the antibacterial immunity of red swamp crayfish (Procambarus clarkii).
abstract: In invertebrates, ficolin-like proteins (FLPs) play important roles in innate immunity against pathogens. Previous studies primarily investigated the functions of FLPs in immune recognition, activation, and regulation. However, limited research has examined the functions of FLPs as immune effectors. In this work, a ficolin-like protein was identified in red swam crayfish (Procambarus clarkii) and designated as PcFLP1. Quantitative RT-PCR and western blot were employed to analyze the distribution and expression profiles of PcFLP1 in the tissues of the crayfish. The results indicated that PcFLP1 was present in all tested tissues, including hemocytes, heart, hepatopancreas, gill, stomach, and mid-intestine. The expression level of PcFLP1 was up-regulated in hemocytes, hepatopancreas and mid-intestines of the crayfish challenged with Vibrio parahaemolyticus. Further study demonstrated that PcFLP1 could protect the hepatopancreatic cells of crayfish from V. parahaemolyticus infection. The recombinant PcFLP1 enhanced bacterial elimination in crayfish, whereas the antibacterial action was inhibited after PcFLP1 was knocked down. Furthermore, PcFLP1 could bound to bacteria and inhibited bacterial replication. These results demonstrated that PcFLP1 plays an important role in the anti-Vibrio immunity of red swamp crayfish.
authors.affiliation: Department of Aquatic Animal Medicine, Research Center for Marine Biology, College of Fisheries, Huazhong Agricultural University, Wuhan, Hubei, 430070, China.
authors.forename: Yun-Jia
authors.initials: YJ
authors.lastname: Dai
authors.affiliation: Department of Aquatic Animal Medicine, Research Center for Marine Biology, College of Fisheries, Huazhong Agricultural University, Wuhan, Hubei, 430070, China.
authors.forename: Yu-Qing
authors.initials: YQ
authors.lastname: Wang
authors.affiliation: Department of Aquatic Animal Medicine, Research Center for Marine Biology, College of Fisheries, Huazhong Agricultural University, Wuhan, Hubei, 430070, China.
authors.forename: Ying-Hao
authors.initials: YH
authors.lastname: Zhang
authors.affiliation: Department of Aquatic Animal Medicine, Research Center for Marine Biology, College of Fisheries, Huazhong Agricultural University, Wuhan, Hubei, 430070, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Department of Aquatic Animal Medicine, Research Center for Marine Biology, College of Fisheries, Huazhong Agricultural University, Wuhan, Hubei, 430070, China.
authors.forename: Jin-Quan
authors.initials: JQ
authors.lastname: Li
authors.affiliation: Department of Aquatic Animal Medicine, Research Center for Marine Biology, College of Fisheries, Huazhong Agricultural University, Wuhan, Hubei, 430070, China.
authors.forename: Shun
authors.initials: S
authors.lastname: Wei
authors.affiliation: Department of Aquatic Animal Medicine, Research Center for Marine Biology, College of Fisheries, Huazhong Agricultural University, Wuhan, Hubei, 430070, China.
authors.forename: Li-Juan
authors.initials: LJ
authors.lastname: Zhao
authors.affiliation: State Key Laboratory of Marine Resource Utilization in South China Sea, Hainan Provincial Key Laboratory for Tropical Hydrobiology and Biotechnology, College of Marine Science, Hainan University, Haikou 570228, China.
authors.forename: Yong-Can
authors.initials: YC
authors.lastname: Zhou
authors.affiliation: Department of Aquatic Animal Medicine, Research Center for Marine Biology, College of Fisheries, Huazhong Agricultural University, Wuhan, Hubei, 430070, China; State Key Laboratory of Marine Resource Utilization in South China Sea, Hainan Provincial Key Laboratory for Tropical Hydrobiology and Biotechnology, College of Marine Science, Hainan University, Haikou 570228, China. Electronic address: linli@mail.hzau.edu.cn.
authors.forename: Li
authors.initials: L
authors.lastname: Lin
authors.affiliation: Department of Aquatic Animal Medicine, Research Center for Marine Biology, College of Fisheries, Huazhong Agricultural University, Wuhan, Hubei, 430070, China. Electronic address: lanjiangfeng@mail.hzau.edu.cn.
authors.forename: Jiang-Feng
authors.initials: JF
authors.lastname: Lan
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D060829:Arthropod Proteins / Q000235:genetics / Q000276:immunology*; D003400:Astacoidea / Q000235:genetics / Q000276:immunology*; D015153:Blotting, Western; D005455:Fluorescent Antibody Technique; D007113:Immunity, Innate / Q000235:genetics / Q000276:immunology*; D037102:Lectins / Q000235:genetics / Q000276:immunology*; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D060888:Real-Time Polymerase Chain Reaction; D000097604:Ficolins
chemical_list: D060829:Arthropod Proteins; D037102:Lectins; D000097604:Ficolins
keywords: Ficolin; Innate immunity; Procambarus clarkii; Vibrio parahaemolyticus