title: Development and Validation of a Sensitive LC-MS-MS Method for Quantification of Mogrol in Rat Plasma and Application to Pharmacokinetic Study.
abstract: Mogrol, the aglycone of mogrosides, is a potential pharmacologically active ingredient isolated from the fruits of Siraitia grosvenorii. The aim of this study was to develop and validate an LC-MS-MS method for the quantification of mogrol in rat plasma. Protein precipitation extraction procedure using methanol/water (1:1, v/v) was employed to extract mogrol from rat plasma. Chromatographic separation was performed on a reverse-phase Agilent Zorbax XDB C18 column (50 mm × 2.1 mm, 3.5 μm) with gradient elution using a mobile phase containing methanol and water, both of which contained 0.1% formic acid at a flow rate of 0.50 mL/min. The analyte was monitored by tandem-mass spectrometry with positive electrospray ionization mode. The precursor/product transitions (m/z) in the positive ion mode were 459.3→423.3 and 386.2→122.3 for mogrol and internal standard, respectively. The method was validated over the concentration range of 10.0-10,000 ng/mL with a lower limit of quantification of 10.0 ng/mL in rat plasma. Validation experiments included tests for specificity, precision, accuracy, matrix effect, and stability under different storage and handling conditions. This method was successfully utilized to pharmacokinetic evaluation of mogrol after intravenous and oral administration of a single dose in rats at 2.0 and 5.0 mg/kg, respectively. The oral absolute bioavailability (F) of mogrol was estimated to be 10.3 ± 2.15% with an elimination half-life (t1/2) value of 2.41 ± 0.11 h.
authors.affiliation: Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, 151 Malianwa North Road, Haidian District, Beijing 100193, P. R. China.
authors.forename: Zuliang
authors.initials: Z
authors.lastname: Luo
authors.affiliation: Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, 151 Malianwa North Road, Haidian District, Beijing 100193, P. R. China.
authors.forename: Kailun
authors.initials: K
authors.lastname: Zhang
authors.affiliation: Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, 151 Malianwa North Road, Haidian District, Beijing 100193, P. R. China.
authors.forename: Hongwu
authors.initials: H
authors.lastname: Shi
authors.affiliation: Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, 151 Malianwa North Road, Haidian District, Beijing 100193, P. R. China.
authors.forename: Yuhua
authors.initials: Y
authors.lastname: Guo
authors.affiliation: Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, 151 Malianwa North Road, Haidian District, Beijing 100193, P. R. China.|Yunnan Branch Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, 138 Xuanwei road, Jinghong 530023, P. R. China.
authors.forename: Xiaojun
authors.initials: X
authors.lastname: Ma
authors.affiliation: Beijing Key Lab of TCM Collateral Disease Theory Research, School of Traditional Chinese Medicine, Capital Medical University, 10 Xitoutiao, Youanmenwai, Fengtai District, Beijing 100069, P. R. China100069, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Qiu
mesh_terms: D061605:Administration, Intravenous; D000284:Administration, Oral; D000818:Animals; D001682:Biological Availability; D056148:Chromatography, Reverse-Phase / Q000379:methods*; D019662:Cucurbitaceae / Q000737:chemistry*; D016014:Linear Models; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D053719:Tandem Mass Spectrometry / Q000379:methods*; D014315:Triterpenes / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics
chemical_list: D014315:Triterpenes