title: Relationship between plasma glycation with membrane modification, oxidative stress and expression of glucose trasporter-1 in type 2 diabetes patients with vascular complications.
abstract: Enhanced protein glycation in diabetes causes irreversible cellular damage through membrane modifications. Erythrocytes are persistently exposed to plasma glycated proteins; however, little are known about its consequences on membrane. Aim of this study was to examine the relationship between plasma protein glycation with erythrocyte membrane modifications in type 2 diabetes patients with and without vascular complications.
  
  
  METHODS
  We recruited 60 healthy controls, 85 type 2 diabetic mellitus (DM) and 75 type 2 diabetic patients with complications (DMC). Levels of plasma glycation adduct with antioxidants (fructosamine, protein carbonyl, β-amyloids, thiol groups, total antioxidant status), erythrocyte membrane modifications (protein carbonyls, β-amyloids, free amino groups, erythrocyte fragility), antioxidant profile (GSH, catalase, lipid peroxidation) and Glut-1 expression were quantified.
  
  
  RESULTS
  Compared with controls, DM and DMC patients had significantly higher level of glycation adducts, erythrocyte fragility, lipid peroxidation and Glut-1 expression whereas declined levels of plasma and cellular antioxidants. Correlation studies revealed positive association of membrane modifications with erythrocyte sedimentation rate, fragility, peroxidation whereas negative association with free amino groups, glutathione and catalase.
  
  
  CONCLUSIONS
  Our data suggest that plasma glycation is associated with oxidative stress, Glut-1 expression and erythrocyte fragility in DM patients. This may further contribute to progression of vascular complications.
authors.affiliation: Biochemical Sciences Division, Rajiv Gandhi Institute of IT and Biotechnology, Bharati Vidyapeeth University, Pune, India.
authors.forename: Krishna A
authors.initials: KA
authors.lastname: Adeshara
authors.affiliation: Department of Medicine, Bharati Vidyapeeth's Medical College and Bharati Hospital, Bharati Vidyapeeth University, Pune, India.
authors.forename: Arundhati G
authors.initials: AG
authors.lastname: Diwan
authors.affiliation: Biochemical Sciences Division, Rajiv Gandhi Institute of IT and Biotechnology, Bharati Vidyapeeth University, Pune, India.
authors.forename: Tejashri R
authors.initials: TR
authors.lastname: Jagtap
authors.affiliation: Biochemical Sciences Division, Rajiv Gandhi Institute of IT and Biotechnology, Bharati Vidyapeeth University, Pune, India.
authors.forename: Komal
authors.initials: K
authors.lastname: Advani
authors.affiliation: Biochemical Sciences Division, Rajiv Gandhi Institute of IT and Biotechnology, Bharati Vidyapeeth University, Pune, India.
authors.forename: Aisha
authors.initials: A
authors.lastname: Siddiqui
authors.affiliation: Biochemical Sciences Division, Rajiv Gandhi Institute of IT and Biotechnology, Bharati Vidyapeeth University, Pune, India. Electronic address: rashmitupe@gmail.com.
authors.forename: Rashmi S
authors.initials: RS
authors.lastname: Tupe
mesh_terms: D000368:Aged; D000975:Antioxidants / Q000378:metabolism; D015415:Biomarkers / Q000097:blood; D001799:Blood Sedimentation; D003924:Diabetes Mellitus, Type 2 / Q000150:complications*; D003925:Diabetic Angiopathies / Q000097:blood / Q000378:metabolism* / Q000503:physiopathology; D018450:Disease Progression; D004910:Erythrocyte Membrane / Q000378:metabolism*; D005260:Female; D051272:Glucose Transporter Type 1 / Q000097:blood / Q000378:metabolism*; D005978:Glutathione / Q000097:blood / Q000737:chemistry / Q000378:metabolism; D006031:Glycosylation; D006801:Humans; D015227:Lipid Peroxidation; D008297:Male; D008875:Middle Aged; D009996:Osmotic Fragility; D010084:Oxidation-Reduction; D018384:Oxidative Stress; D050050:Protein Carbonylation
chemical_list: D000975:Antioxidants; D015415:Biomarkers; D051272:Glucose Transporter Type 1; C495677:SLC2A1 protein, human; D005978:Glutathione
keywords: Diabetes; Erythrocytes; Glut-1; Glycation; Membrane modifications; Oxidative stress