title: Inhibition of key digestive enzymes related to hyperlipidemia and protection of liver-kidney functions by Cystoseira crinita sulphated polysaccharide in high-fat diet-fed rats.
abstract: The objective of this current study was to investigate the possible hyperlipidemic and antioxidative effects of Cystoseira crinita sulfated polysaccharide (CCSP) in rats fed with a high-fat diet, exhibited an inhibitory activity on pancreatic lipase in vitro. In vivo administration of this extract to HFD-rats lowered body weight and potentially inhibited key enzymes of lipid metabolism and absorption as lipase activity in both plasma and small intestine, which led to a notable decrease of blood LDL- cholesterol (LDL-Ch) and triglycerides (TG) levels, and an increase in HDL-cholesterol (HDL-Ch) levels in HFD-rats. CCSP was also observed to protect the liver-kidney functions efficiently, by decreasing of aspartate transaminase (AST), alanine transaminase (ALT), lactate dehydrogenase (LDH) and Creatine phosphokinase (CPK) activities and creatinine, albumin, T-bilirubin, uric acid, and urea rates in plasma. The histological analysis of liver and kidney tissues further established the positive effect of CCSP.
authors.affiliation: Laboratory of Plant Biotechnology Applied to the Improvement of Cultures, Faculty of Sciences of Sfax, Sfax, Tunisia; Stazione Zoologica 'A. Dohrn', Functional and Evolutionary Ecology Laboratory, Punta S. Pietro, Ischia Naples, Italy,. Electronic address: bengara_amel@yahoo.fr.
authors.forename: Amel
authors.initials: A
authors.lastname: Ben Gara
authors.affiliation: Laboratory of Plant Biotechnology Applied to the Improvement of Cultures, Faculty of Sciences of Sfax, Sfax, Tunisia.
authors.forename: Rihab
authors.initials: R
authors.lastname: Ben Abdallah Kolsi
authors.affiliation: Biochemistry Laboratory, CHU Hedi Chaker, Sfax, Tunisia.
authors.forename: Rim
authors.initials: R
authors.lastname: Chaaben
authors.affiliation: Laboratory of Plant Biodiversity and Dynamic of Ecosystems in Arid Area, Faculty of Sciences of Sfax, Sfax, Tunisia.
authors.forename: Nadia
authors.initials: N
authors.lastname: Hammami
authors.affiliation: Laboratory of Chemistry of Natural Products, Faculty of Sciences, University of Sfax, B.P. 1171, 3000 Sfax, Tunisia.
authors.forename: Majed
authors.initials: M
authors.lastname: Kammoun
authors.affiliation: Stazione Zoologica 'A. Dohrn', Functional and Evolutionary Ecology Laboratory, Punta S. Pietro, Ischia Naples, Italy.
authors.forename: Francesco
authors.initials: F
authors.lastname: Paolo Patti
authors.affiliation: Laboratory of Animal Ecophysiology, Faculty of Sciences of Sfax, Tunisia.
authors.forename: Abdelfattah
authors.initials: A
authors.lastname: El Feki
authors.affiliation: Laboratory of Plant Biotechnology Applied to the Improvement of Cultures, Faculty of Sciences of Sfax, Sfax, Tunisia.
authors.forename: Lotfi
authors.initials: L
authors.lastname: Fki
authors.affiliation: Laboratory of Molecular Biology of Eukaryotes Center of Biotechnology of Sfax, Tunisia.
authors.forename: Hafedh
authors.initials: H
authors.lastname: Belghith
authors.affiliation: Laboratory of Plant Biotechnology Applied to the Improvement of Cultures, Faculty of Sciences of Sfax, Sfax, Tunisia.
authors.forename: Karima
authors.initials: K
authors.lastname: Belghith
mesh_terms: D000818:Animals; D004041:Dietary Fats / Q000008:administration & dosage / Q000494:pharmacology*; D004063:Digestion / Q000187:drug effects / Q000502:physiology; D004305:Dose-Response Relationship, Drug; D006949:Hyperlipidemias / Q000188:drug therapy*; D007668:Kidney / Q000187:drug effects; D008099:Liver / Q000187:drug effects; D008297:Male; D000459:Phaeophyceae / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry*; D011134:Polysaccharides / Q000008:administration & dosage / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D004041:Dietary Fats; D010936:Plant Extracts; D011134:Polysaccharides
keywords: Antioxidant activity; Liver-kidney functions; Obesity; Polysaccharide