title: An Investigation on Micro-Raman Spectra and Wavelet Data Analysis for Pemphigus Vulgaris Follow-up Monitoring.
abstract: A wavelet multi-component decomposition algorithm has been used for data analysis of micro-Raman spectra of blood serum samples from patients affected by pemphigus vulgaris at different stages. Pemphigus is a chronic, autoimmune, blistering disease of the skin and mucous membranes with a potentially fatal outcome. Spectra were measured by means of a Raman confocal microspectrometer apparatus using the 632.8 nm line of a He-Ne laser source. A discrete wavelet transform decomposition method has been applied to the recorded Raman spectra in order to overcome problems related to low-level signals and the presence of noise and background components due to light scattering and fluorescence. This numerical data treatment can automatically extract quantitative information from the Raman spectra and makes more reliable the data comparison. Even if an exhaustive investigation has not been done in this work, the feasibility of the follow-up monitoring of pemphigus vulgaris pathology has been clearly proved with useful implications for the clinical applications.
authors.affiliation: Consiglio Nazionale delle Ricerche, Istituto di Cibernetica "E. Caianiello", Pozzuoli, Italy.
authors.forename: Carlo
authors.initials: C
authors.lastname: Camerlingo
authors.affiliation: Dipartimento di Scienze Fisiche, Università "Federico II", Naples, Italy.
authors.forename: Flora
authors.initials: F
authors.lastname: Zenone
authors.affiliation: Dipartimento di Scienze Biomediche, Università di Foggia, Italy.
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Perna
authors.affiliation: Dipartimento di Scienze Biomediche, Università di Foggia, Italy.
authors.forename: Vito
authors.initials: V
authors.lastname: Capozzi
authors.affiliation: Dipartimento di Malattie Odontostomatologiche, Seconda Università di Napoli, Naples, Italy.
authors.forename: Nicola
authors.initials: N
authors.lastname: Cirillo
authors.affiliation: Dipartimento di Malattie Odontostomatologiche, Seconda Università di Napoli, Naples, Italy.
authors.forename: Giovanni Maria
authors.initials: GM
authors.lastname: Gaeta
authors.affiliation: Dipartimento di Medicina Sperimentale, Seconda Università di Napoli, Naples, Italy. maria.lepore@unina2.it.
authors.forename: Maria
authors.initials: M
authors.lastname: Lepore
keywords: blood serum; micro-Raman spectroscopy; wavelet analysis