title: Growth inhibitory and proapoptotic effects of l-asparaginase from Fusarium culmorum ASP-87 on human leukemia cells (Jurkat).
abstract: The objective of this study was to evaluate the anticancer properties of l-asparaginase purified from fungal isolate Fusarium culmorum ASP-87 against human T-cell leukemia cell line (Jurkat). The growth inhibitory and proapoptotic effects of purified l-asparaginase on Jurkat cell lines were investigated by determining its influence on cell viability, colony formation, DNA fragmentation, and cell cycle progression. The results revealed that purified l-asparaginase showed significant decrease in cell survival with IC50value of 90 μg/mL (9 IU/mL). The enzyme inhibited colony formation and showed characteristic laddering pattern on agarose gel thereby confirming the induction of apoptosis. Further, cell cycle analysis revealed that the enzyme induced apoptotic cell death by arresting the growth of cells at G2-M phase. However, the enzyme did not elicit any toxic effects on human erythrocytes. l-asparaginase purified from F. culmorum ASP-87 showed significant and selective cytotoxic and apoptotic effects on human T-cell leukemic cells in dose-dependent manner. Results of the study give leads for the anticancer effects of fungal l-asparaginase and its potential usefulness in the chemotherapy of leukemia.
authors.affiliation: Department of Microbiology and Biotechnology, Bangalore University, Jnana Bharathi Campus, Bangaluru, 560056, Karnataka, India.
authors.forename: Anil K
authors.initials: AK
authors.lastname: Meghavarnam
authors.affiliation: Centre of Advanced Study in Marine Biology, Faculty of Marine Sciences, Annamalai University, Parangipettai, 608502, Tamil Nadu, India.
authors.forename: Maryam
authors.initials: M
authors.lastname: Salah
authors.affiliation: Department of Microbiology and Biotechnology, Bangalore University, Jnana Bharathi Campus, Bangaluru, 560056, Karnataka, India.
authors.forename: Meenakshisundaram
authors.initials: M
authors.lastname: Sreepriya
authors.affiliation: Department of Microbiology and Biotechnology, Bangalore University, Jnana Bharathi Campus, Bangaluru, 560056, Karnataka, India.
authors.forename: Savitha
authors.initials: S
authors.lastname: Janakiraman
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D001215:Asparaginase / Q000494:pharmacology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects; D053938:DNA Fragmentation / Q000187:drug effects; D004912:Erythrocytes / Q000187:drug effects; D005670:Fusarium / Q000737:chemistry*; D059565:G2 Phase Cell Cycle Checkpoints / Q000187:drug effects; D006801:Humans; D019169:Jurkat Cells; D007938:Leukemia / Q000188:drug therapy*
chemical_list: D000970:Antineoplastic Agents; D001215:Asparaginase
keywords: 
  Fusarium culmorum
  ; DNA fragmentation; apoptosis; cell cycle; leukemia