title: Extra-intestinal detection of canine kobuvirus in a puppy from Southern Brazil.
abstract: This study presents the pathological, immunohistochemical, and molecular findings associated with the extra-intestinal detection of canine kobuvirus (CaKV) in a 5-month-old Chihuahua puppy, that had a clinical history of bloody-tinged feces. Principal pathological findings were interstitial pneumonia, necrotizing bronchitis, and parvovirus-induced enteritis. Molecular diagnostic methods identified CaKV within the cerebellum, cerebrum, lung, tonsil, and liver. CaKV and rotavirus were not identified within the feces and intestine. Immunohistochemistry (IHC) assays detected antigens of CDV and CAdV-1 in the lungs. These results confirmed the extra-intestinal detection of CaKV in this puppy and represent the first extra-intestinal detection of CaKV in a dog.
authors.affiliation: Laboratory of Animal Virology, Department of Veterinary Preventive Medicine, Universidade Estadual de Londrina, Rodovia Celso Garcia Cid, Campus Universitario, PO Box 10011, Londrina, PR, 86057-970, Brazil.
authors.forename: Juliane
authors.initials: J
authors.lastname: Ribeiro
authors.affiliation: Laboratory of Animal Pathology, Department of Veterinary Preventive Medicine, Universidade Estadual de Londrina, Londrina, Brazil.
authors.forename: Selwyn Arlington
authors.initials: SA
authors.lastname: Headley
authors.affiliation: Laboratory of Animal Virology, Department of Veterinary Preventive Medicine, Universidade Estadual de Londrina, Rodovia Celso Garcia Cid, Campus Universitario, PO Box 10011, Londrina, PR, 86057-970, Brazil.
authors.forename: Jaqueline Assumpção
authors.initials: JA
authors.lastname: Diniz
authors.affiliation: Laboratory of Animal Pathology, Department of Veterinary Preventive Medicine, Universidade Estadual de Londrina, Londrina, Brazil.
authors.forename: Alfredo Hajime Tanaka
authors.initials: AH
authors.lastname: Pereira
authors.affiliation: Laboratory of Animal Virology, Department of Veterinary Preventive Medicine, Universidade Estadual de Londrina, Rodovia Celso Garcia Cid, Campus Universitario, PO Box 10011, Londrina, PR, 86057-970, Brazil.|Multi-User Animal Health Laboratory, Molecular Biology Unit, Universidade Estadual de Londrina, Londrina, Brazil.
authors.forename: Elis
authors.initials: E
authors.lastname: Lorenzetti
authors.affiliation: Laboratory of Animal Virology, Department of Veterinary Preventive Medicine, Universidade Estadual de Londrina, Rodovia Celso Garcia Cid, Campus Universitario, PO Box 10011, Londrina, PR, 86057-970, Brazil. alfieri@uel.br.
authors.forename: Amauri Alcindo
authors.initials: AA
authors.lastname: Alfieri
authors.affiliation: Laboratory of Animal Virology, Department of Veterinary Preventive Medicine, Universidade Estadual de Londrina, Rodovia Celso Garcia Cid, Campus Universitario, PO Box 10011, Londrina, PR, 86057-970, Brazil.|Multi-User Animal Health Laboratory, Molecular Biology Unit, Universidade Estadual de Londrina, Londrina, Brazil.
authors.forename: Alice Fernandes
authors.initials: AF
authors.lastname: Alfieri
mesh_terms: D000818:Animals; D001938:Brazil; D004283:Dog Diseases / Q000473:pathology / Q000821:virology*; D004285:Dogs; D004751:Enteritis / Q000473:pathology / Q000662:veterinary / Q000821:virology; D005243:Feces / Q000821:virology; D005260:Female; D007422:Intestines / Q000473:pathology / Q000821:virology; D052699:Kobuvirus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D008297:Male; D010802:Phylogeny; D010850:Picornaviridae Infections / Q000473:pathology / Q000662:veterinary* / Q000821:virology