title: Overexpression of suppressor of IKBKE 1 is associated with vincristine resistance in colon cancer cells.
abstract: In a previous study, the suppressor of IKBKE 1 expression level was confirmed to be higher in vincristine (VCR)-resistant HCT-8 (HCT-8/V) colon cancer cells than in non-VCR-resistant HCT-8 cells. In the current study, IKBKE 1 expression in VCR-resistant colon cancer cells was investigated further. HCT-8 and HCT-8/V human colon cancer cells were used, and polymerase chain reaction (PCR) primers were designed to amplify theIKBKE 1gene. Fluorescence reverse transcription-quantitative PCR (RT-qPCR) was performed to detect differences inIKBKE 1expression between sensitive and drug-resistant colon cancer cell lines. Western blotting was performed to further observe IKBKE 1 expression. Based on the RT-qPCR and western blot results, IKBKE 1 expression was observed to be markedly higher in the HCT-8/V cells, and this difference was significant (P<0.05). Thus,IKBKE 1expression was identified to be associated with the resistance of colon cancer cells to VCR.
authors.affiliation: Department of Biochemistry and Molecular Biology, Xinxiang Medical University, Xinxiang, Henan 453003, P.R. China.
authors.forename: Chun-Peng
authors.initials: CP
authors.lastname: Zhao
authors.affiliation: Department of Life Science and Technology, Xinxiang Medical University, Xinxiang, Henan 453003, P.R. China.
authors.forename: Zhong-Jie
authors.initials: ZJ
authors.lastname: Xu
authors.affiliation: Department of College of Basic Medicine, Xinxiang Medical University, Xinxiang, Henan 453003, P.R. China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Guo
authors.affiliation: Department of Life Science and Technology, Xinxiang Medical University, Xinxiang, Henan 453003, P.R. China.
authors.forename: Yun-Xiao
authors.initials: YX
authors.lastname: Li
authors.affiliation: Department of Life Science and Technology, Xinxiang Medical University, Xinxiang, Henan 453003, P.R. China.
authors.forename: Xiang-Zheng
authors.initials: XZ
authors.lastname: Gao
authors.affiliation: Department of Life Science and Technology, Xinxiang Medical University, Xinxiang, Henan 453003, P.R. China.
authors.forename: Yi-You
authors.initials: YY
authors.lastname: Peng
keywords: HCT-8; colon cancer; drug resistance; suppressor of IKBKE 1; vincristine