title: Adoptive transfer of T regulatory cells inhibits lipopolysaccharide-induced inflammation in fetal brain tissue in a late-pregnancy preterm birth mouse model.
abstract: To evaluate the effect of regulatory T cells (Tregs) on the inflammation resulting from lipopolysaccharide (LPS) challenge in prenatal brain tissue, Tregs isolated from pregnant mice were transferred into model mice, and the expression levels of fork head family transcription factor (Foxp3), interleukin-6 (IL-6), CD68 (a marker of microglia), and toll-like receptor 4 (TLR-4) were assessed in the fetal brain tissue. Foxp3, IL-6, and TLR-4 expression were detected by polymerase chain reaction and Western blot; CD68 expression level was detected using immunochemical analysis. Foxp3, IL-6, TLR-4, and CD68 expressions in fetal brain were significantly induced by maternal LPS administration, and the increased expression levels were markedly reduced by adoptive transfer of Tregs. Maternal LPS exposure significantly induced inflammation in perinatal brain tissue, and Tregs negatively regulated this LPS-induced inflammation.
authors.affiliation: Department of Neonatology, College of Medicine, The First Affiliated Hospital, Xi'an Jiaotong University, Yanta West Road No. 277, Xian 710049, Shanxi Province, China.
authors.forename: Fan
authors.initials: F
authors.lastname: Wang
authors.affiliation: Department of Neonatology, College of Medicine, The First Affiliated Hospital, Xi'an Jiaotong University, Yanta West Road No. 277, Xian 710049, Shanxi Province, China.
authors.forename: Mi
authors.initials: M
authors.lastname: Xiao
authors.affiliation: Department of Neonatology, College of Medicine, The First Affiliated Hospital, Xi'an Jiaotong University, Yanta West Road No. 277, Xian 710049, Shanxi Province, China.
authors.forename: Ru-Juan
authors.initials: RJ
authors.lastname: Chen
authors.affiliation: Department of Neonatology, College of Medicine, The First Affiliated Hospital, Xi'an Jiaotong University, Yanta West Road No. 277, Xian 710049, Shanxi Province, China.
authors.forename: Xiao-Jie
authors.initials: XJ
authors.lastname: Lin
authors.affiliation: Department of Neonatology, College of Medicine, The First Affiliated Hospital, Xi'an Jiaotong University, Yanta West Road No. 277, Xian 710049, Shanxi Province, China.
authors.forename: Muhammad
authors.initials: M
authors.lastname: Siddiq
authors.affiliation: Department of Neonatology, College of Medicine, The First Affiliated Hospital, Xi'an Jiaotong University, Yanta West Road No. 277, Xian 710049, Shanxi Province, China.
authors.forename: Li
authors.initials: L
authors.lastname: Liu
mesh_terms: D019264:Adoptive Transfer; D000818:Animals; D015703:Antigens, CD / Q000235:genetics / Q000378:metabolism; D015214:Antigens, Differentiation, Myelomonocytic / Q000235:genetics / Q000378:metabolism; D001921:Brain / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D004195:Disease Models, Animal; D005260:Female; D005333:Fetus / Q000187:drug effects / Q000378:metabolism; D051858:Forkhead Transcription Factors / Q000235:genetics / Q000378:metabolism; D007249:Inflammation / Q000517:prevention & control; D015850:Interleukin-6 / Q000235:genetics / Q000378:metabolism; D008070:Lipopolysaccharides / Q000633:toxicity*; D051379:Mice; D017628:Microglia / Q000378:metabolism; D011247:Pregnancy; D047928:Premature Birth; D050378:T-Lymphocytes, Regulatory / Q000166:cytology / Q000276:immunology* / Q000378:metabolism; D051197:Toll-Like Receptor 4 / Q000235:genetics / Q000378:metabolism; D015854:Up-Regulation / Q000187:drug effects; D000097382:CD68 Molecule
chemical_list: D015703:Antigens, CD; D015214:Antigens, Differentiation, Myelomonocytic; D051858:Forkhead Transcription Factors; D015850:Interleukin-6; D008070:Lipopolysaccharides; D051197:Toll-Like Receptor 4; C081026:CD68 protein, mouse; D000097382:CD68 Molecule; C498833:Foxp3 protein, mouse
keywords: lipopolysaccharide; microglia; perinatal brain; regulatory T cells