title: Modern Approaches to Studies of New Osteogenic Biomaterials on the Model of Regeneration of Critical-Size Cranial Defects in Rats.
abstract: Osteoinductive characteristics of new osteoplastic materials based on demineralized bone matrix of xenogenic origin with high and controlled degree of purification were studied on the model of regeneration of critical-size cranial defects in rats using modern approaches, including histological analysis, evaluation of morphological parameters of the bone tissue obtained by micro-computed tomography, and estimation of bone tissue growth rate using in vivo fluorochrome label. Demineralized bone matrix and, to a much greater extent, its activated form containing modified recombinant growth factor rhBMP-2 with high content of the dimeric form exhibited osteoinductive activity.
authors.affiliation: N. F. Gamaleya Federal Research Center of Epidemiology and Microbiology, Ministry of Health of the Russian Federation, Moscow, Russia. mike.bartov@gmail.com.
authors.forename: M S
authors.initials: MS
authors.lastname: Bartov
authors.affiliation: N. F. Gamaleya Federal Research Center of Epidemiology and Microbiology, Ministry of Health of the Russian Federation, Moscow, Russia.
authors.forename: A V
authors.initials: AV
authors.lastname: Gromov
authors.affiliation: N. F. Gamaleya Federal Research Center of Epidemiology and Microbiology, Ministry of Health of the Russian Federation, Moscow, Russia.
authors.forename: M S
authors.initials: MS
authors.lastname: Poponova
authors.affiliation: N. F. Gamaleya Federal Research Center of Epidemiology and Microbiology, Ministry of Health of the Russian Federation, Moscow, Russia.
authors.forename: D M
authors.initials: DM
authors.lastname: Savina
authors.affiliation: N. F. Gamaleya Federal Research Center of Epidemiology and Microbiology, Ministry of Health of the Russian Federation, Moscow, Russia.
authors.forename: K E
authors.initials: KE
authors.lastname: Nikitin
authors.affiliation: N. F. Gamaleya Federal Research Center of Epidemiology and Microbiology, Ministry of Health of the Russian Federation, Moscow, Russia.
authors.forename: T M
authors.initials: TM
authors.lastname: Grunina
authors.affiliation: A. N. Belozersky Institute of Physico-Chemical Biology, M. V. Lomonosov Moscow State University, Moscow, Russia.
authors.forename: V N
authors.initials: VN
authors.lastname: Manskikh
authors.affiliation: N. F. Gamaleya Federal Research Center of Epidemiology and Microbiology, Ministry of Health of the Russian Federation, Moscow, Russia.
authors.forename: O A
authors.initials: OA
authors.lastname: Gra
authors.affiliation: N. F. Gamaleya Federal Research Center of Epidemiology and Microbiology, Ministry of Health of the Russian Federation, Moscow, Russia.
authors.forename: V G
authors.initials: VG
authors.lastname: Lunin
authors.affiliation: N. F. Gamaleya Federal Research Center of Epidemiology and Microbiology, Ministry of Health of the Russian Federation, Moscow, Russia.|A. N. Belozersky Institute of Physico-Chemical Biology, M. V. Lomonosov Moscow State University, Moscow, Russia.
authors.forename: A S
authors.initials: AS
authors.lastname: Karyagina
authors.affiliation: N. F. Gamaleya Federal Research Center of Epidemiology and Microbiology, Ministry of Health of the Russian Federation, Moscow, Russia.
authors.forename: A L
authors.initials: AL
authors.lastname: Gintsburg
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000494:pharmacology; D018775:Bone Demineralization Technique / Q000379:methods*; D055396:Bone Morphogenetic Protein 2 / Q000096:biosynthesis / Q000235:genetics / Q000494:pharmacology*; D001861:Bone Regeneration / Q000187:drug effects*; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D005456:Fluorescent Dyes; D015870:Gene Expression; D006801:Humans; D055767:Immobilized Proteins / Q000096:biosynthesis / Q000235:genetics / Q000494:pharmacology; D008297:Male; D010012:Osteogenesis / Q000187:drug effects*; D055503:Protein Multimerization; D051381:Rats; D017208:Rats, Wistar; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics / Q000494:pharmacology; D012886:Skull / Q000187:drug effects* / Q000293:injuries / Q000601:surgery; D023822:Tissue Engineering; D054457:Tissue Scaffolds; D055114:X-Ray Microtomography
chemical_list: C525718:BMP2 protein, human; D001672:Biocompatible Materials; D055396:Bone Morphogenetic Protein 2; D005456:Fluorescent Dyes; D055767:Immobilized Proteins; D011994:Recombinant Proteins
keywords: BMP-2; bone tissue regeneration; defect of critical size; demineralized bone matrix; osteoinductive activity