title: Molecular detection ofToxoplasma gondiiin house sparrow (Passer domesticus) by LAMP and PCR methods in Tehran, Iran.
abstract: Toxoplasma gondiiis one of the most common zoonotic parasitic diseases in human and warm-blooded animals worldwide. Birds are one of important intermediate hosts ofT. gondii. The aim of this study is molecular detection ofT. gondiiin the house sparrow by LAMP and PCR methods in Tehran, Iran. A total 200 sparrows were captured in different regions of Tehran. DNA was extracted from tissue samples of each sparrow. LAMP and conventional PCR assays were carried out with a set of primers to detect the 529 bp fragment ofT. gondii. LAMP and PCR were detectedT. gondiifrom 17 (8.5 %) and 15 (7.5 %) of 200 sparrows respectively. These results indicated that sensitivity of LAMP was higher than conventional PCR. In our knowledge, this study is the first report of detection ofT. gondiiby LAMP method in bird hosts. Also, these findings provided an insight into epidemiological pattern ofT. gondiiinfection in sparrow in Iran.
authors.affiliation: Department of Parasitology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
authors.forename: Amir
authors.initials: A
authors.lastname: Abdoli
authors.affiliation: Department of Parasitology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
authors.forename: Abdolhossein
authors.initials: A
authors.lastname: Dalimi
authors.affiliation: Reproductive Biotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran.
authors.forename: Haleh
authors.initials: H
authors.lastname: Soltanghoraee
authors.affiliation: Department of Parasitology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
authors.forename: Fatemeh
authors.initials: F
authors.lastname: Ghaffarifar
keywords: Iran; Loop-mediated isothermal amplification (LAMP); PCR; Sparrow; Toxoplasma gondii