title: Diagnosis of Intoxication by the Organophosphate VX: Comparison Between an Electrochemical Sensor and Ellman´s Photometric Method.
abstract: An electrochemical sensor is introduced as a tool applicable for diagnosis of intoxication by cholinesterase inhibitors caused by the well-known nerve agent VX. The traditional Ellman method was chosen for comparison with the sensor's analytical parameters. Both methods are based on estimation of blood cholinesterase inhibition as a marker of intoxication. While Ellman´s method provided a limit of detection of 5.2´10-7M for blood containing VX, the electrochemical sensor was able to detect 4.0´10-7M. Good correlation between both methods was observed (R = 0.92). The electrochemical sensor could be considered a convenient tool for a fast yet accurate method, easily available for field as well as laboratory use. Time and cost savings are key features of the sensor-based assay.
authors.affiliation: Centre of Advanced Studies and Department of Toxicology, Faculty of Military Health Sciences, University of Defense / Trebesska 1575, 50001 Hradec Kralove, Czech Republic. rau@atlas.cz.
authors.forename: Miroslav
authors.initials: M
authors.lastname: Pohanka
authors.affiliation: Centre of Advanced Studies and Department of Toxicology, Faculty of Military Health Sciences, University of Defense / Trebesska 1575, 50001 Hradec Kralove, Czech Republic. hrabinova@pmfhk.cz.
authors.forename: Martina
authors.initials: M
authors.lastname: Hrabinova
authors.affiliation: Centre of Advanced Studies and Department of Toxicology, Faculty of Military Health Sciences, University of Defense / Trebesska 1575, 50001 Hradec Kralove, Czech Republic. kucakam@pmfhk.cz.
authors.forename: Kamil
authors.initials: K
authors.lastname: Kuca
keywords: Organophosphate; biosensor; carbamate; diagnosis; intoxication