title: Bio-Guided Isolation of Methanol-Soluble Metabolites of Common Spruce (Picea abies) Bark by-Products and Investigation of Their Dermo-Cosmetic Properties.
abstract: Common spruce (Picea abiesL.) is a fast-growing coniferous tree, widely used in several countries for the production of sawn wood, timber and pulp. During this industrial exploitation, large quantities of barks are generated as waste materials. The aim of this study was the bio-guided investigation and the effective recovery of methanol-soluble metabolites of common spruce bark for the development of new dermo-cosmetic agents. The active methanol extract was initially fractionated by Centrifugal Partition Chromatography (CPC) using a triphasic solvent system in a step-gradient elution mode. All resulting fractions were evaluated for their antibacterial activity, antioxidant activity and their capability to inhibit tyrosinase, elastase and collagenase activity. In parallel, the chemical composition of each fraction was established by combining a13C-NMR dereplication approach and 2D-NMR analyses. As a result, fourteen secondary metabolites corresponding to stilbene, flavonoid and phenolic acid derivatives were directly identified in the CPC fractions. A high amount (0.93 g) ofE-astringin was recovered from 3 g of crude extract in a single 125 min run.E-Astringin significantly induced the tyrosinase activity whileE-piceid, taxifolin, and taxifolin-3'-O-glucopyranoside exhibited significant anti-tyrosinase activity. The above compounds showed important anti-collagenase and antimicrobial activities, thus providing new perspectives for potential applications as cosmetic ingredients.
authors.affiliation: Institut de Chimie Moléculaire de Reims, UMR CNRS 7312, SFR CAP'SANTE, UFR de Pharmacie, Université de Reims Champagne-Ardenne, Reims 51687, France. aangjel@pharm.uoa.gr.|Division of Pharmacognosy and Natural Products Chemistry, School of Pharmacy, University of Athens, Panepistimioupolis, Zografou, Athens 15771, Greece. aangjel@pharm.uoa.gr.
authors.forename: Apostolis
authors.initials: A
authors.lastname: Angelis
authors.affiliation: Institut de Chimie Moléculaire de Reims, UMR CNRS 7312, SFR CAP'SANTE, UFR de Pharmacie, Université de Reims Champagne-Ardenne, Reims 51687, France. jane.hubert@univ-reims.fr.
authors.forename: Jane
authors.initials: J
authors.lastname: Hubert
authors.affiliation: Division of Pharmacognosy and Natural Products Chemistry, School of Pharmacy, University of Athens, Panepistimioupolis, Zografou, Athens 15771, Greece. aligiannis@pharm.uoa.gr.
authors.forename: Nektarios
authors.initials: N
authors.lastname: Aligiannis
authors.affiliation: Division of Pharmacognosy and Natural Products Chemistry, School of Pharmacy, University of Athens, Panepistimioupolis, Zografou, Athens 15771, Greece. rozaliamich@pharm.uoa.gr.
authors.forename: Rozalia
authors.initials: R
authors.lastname: Michalea
authors.affiliation: Institut de Chimie Moléculaire de Reims, UMR CNRS 7312, SFR CAP'SANTE, UFR de Pharmacie, Université de Reims Champagne-Ardenne, Reims 51687, France. amin.abedini@univ-reims.fr.
authors.forename: Amin
authors.initials: A
authors.lastname: Abedini
authors.affiliation: Institut de Chimie Moléculaire de Reims, UMR CNRS 7312, SFR CAP'SANTE, UFR de Pharmacie, Université de Reims Champagne-Ardenne, Reims 51687, France. jean-marc.nuzillard@univ-reims.fr.
authors.forename: Jean-Marc
authors.initials: JM
authors.lastname: Nuzillard
authors.affiliation: Biomatériaux et Inflammation en Site Osseux, EA 4691, SFR CAP-Santé, UFR de Pharmacie, Université de Reims Champagne Ardenne, Reims 51100, France. sophie.gangloff@univ-reims.fr.
authors.forename: Sophie C
authors.initials: SC
authors.lastname: Gangloff
authors.affiliation: Division of Pharmacognosy and Natural Products Chemistry, School of Pharmacy, University of Athens, Panepistimioupolis, Zografou, Athens 15771, Greece. skaltsounis@pharm.uoa.gr.
authors.forename: Alexios-Leandros
authors.initials: AL
authors.lastname: Skaltsounis
authors.affiliation: Institut de Chimie Moléculaire de Reims, UMR CNRS 7312, SFR CAP'SANTE, UFR de Pharmacie, Université de Reims Champagne-Ardenne, Reims 51687, France. jh.renault@univ-reims.fr.
authors.forename: Jean-Hugues
authors.initials: JH
authors.lastname: Renault
mesh_terms: D000890:Anti-Infective Agents / Q000737:chemistry / Q000494:pharmacology; D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology; D005591:Chemical Fractionation / Q000379:methods; D003358:Cosmetics / Q000737:chemistry* / Q000302:isolation & purification*; D004791:Enzyme Inhibitors / Q000737:chemistry / Q000494:pharmacology; D009682:Magnetic Resonance Spectroscopy; D000432:Methanol / Q000737:chemistry*; D008826:Microbial Sensitivity Tests; D028222:Picea / Q000737:chemistry*; D024301:Plant Bark / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry* / Q000302:isolation & purification* / Q000494:pharmacology; D012995:Solubility
chemical_list: D000890:Anti-Infective Agents; D000975:Antioxidants; D003358:Cosmetics; D004791:Enzyme Inhibitors; D010936:Plant Extracts; D000432:Methanol
keywords: 13C-NMR dereplication; Centrifugal Partition Chromatography; E-astringin; dermo-cosmetic agents; taxifolin; tyrosinase, elastase and collagenase activity