title: Effect of Th1/Th2 cytokine administration on proinflammatory SKOV-3 cell activation.
abstract: BACKGROUND
  Interleukin(IL)-1β, IL-6 and IL-12 might associate with inflammatory processes in a tumor progression and create a specific microenvironment for tumor growth. The aim of the study was to assess whether the Th1 and Th2 type cytokines, such as IL-2 and IL-10, affect ovarian carcinoma continuous cell line (SKOV-3) pro-inflammatory activation.
  
  
  METHODS
  SKOV-3 ovarian cells and peripheral blood mononuclear cells (PBMCs) were stimulated by IL-2 and IL-10. Additionally, SKOV-3 ovarian cells and PBMCs were co-cultured together. Proinflammatory activation of cancer cells was evaluated by measurement of IL-1β and IL-6 levels in culture fluid after 72 h of incubation.
  
  
  RESULTS
  SKOV-3 cells and PBMCs secreted IL-1β and IL-6. After stimulation by IL-2 and IL-10, secretion of studied parameters was changed in a dose-dependent manner. The addition of a higher IL-2 level gave rise to an increase of IL-1β, IL-6 and IL-12 secretion in SKOV-3 cells. Stimulation by IL-10 increased only IL-1β secretion in SKOV-3 cells. However, IL-6 secretion decreased after stimulation with 25 ng/ml IL-10. Activatory effects of IL-2 and inhibitory effects of IL-10 in co-culture of SKOV-3 and PBMCs were observed.
  
  
  CONCLUSIONS
  Our results suggested that Th1/Th2 type of cytokines might influence pro-inflammatory activation of SKOV-3 ovarian cells. Co-cultures of SKOV-3 and PBMCs showed significant changes in cross-talk between cancer and immune cells.
authors.affiliation: Department of Immunology and Serology, School of Pharmacy with the Division of Medical Analytics in Sosnowiec, Medical University of Silesia, Katowice, Poland.
authors.forename: Aleksandra
authors.initials: A
authors.lastname: Mielczarek-Palacz
authors.affiliation: Department of Immunology and Serology, School of Pharmacy with the Division of Medical Analytics in Sosnowiec, Medical University of Silesia, Katowice, Poland.
authors.forename: Justyna
authors.initials: J
authors.lastname: Sikora
authors.affiliation: Department of Immunology and Serology, School of Pharmacy with the Division of Medical Analytics in Sosnowiec, Medical University of Silesia, Katowice, Poland.
authors.forename: Zdzisława
authors.initials: Z
authors.lastname: Kondera-Anasz
authors.affiliation: X-ray Microtomography Laboratory, Department of Biomedical Computer Systems, Institute of Computer Science, Faculty of Computer Science and Material Science, University of Silesia, Katowice, Poland.
authors.forename: Patrycja
authors.initials: P
authors.lastname: Mickiewicz
authors.affiliation: Department of Immunology and Serology, School of Pharmacy with the Division of Medical Analytics in Sosnowiec, Medical University of Silesia, Katowice, Poland.
authors.forename: Adam
authors.initials: A
authors.lastname: Mickiewicz
keywords: IL-12; IL-1β; IL-6; Th1-Th2 cytokines; ovarian cancer SKOV-3 cells