title: Cytocompatible and multifunctional polymeric nanoparticles for transportation of bioactive molecules into and within cells.
abstract: Multifunctional polymeric nanoparticles are materials with great potential for a wide range of biomedical applications. For progression in this area of research, unfavorable interactions of these nanoparticles with proteins and cells must be avoided in biological environments, for example, through treatment of the nanoparticle surfaces. Construction of an artificial cell membrane structure based on polymers bearing the zwitterionic phosphorylcholine group can prevent biological reactions at the surface effectively. In addition, certain bioactive molecules can be immobilized on the surface of the polymer to generate enough affinity to capture target biomolecules. Furthermore, entrapment of inorganic nanoparticles inside polymeric matrices enhances the nanoparticle functionality significantly. This review summarizes the preparation and characterization of cytocompatible and multifunctional polymeric nanoparticles; it analyzes the efficiency of their fluorescence function, the nature of the artificial cell membrane structure, and their performance as in-cell devices; and finally, it evaluates both their chemical reactivity and effects in cells.
authors.affiliation: Department of Materials Engineering, School of Engineering, The University of Tokyo, Tokyo, Japan; Department of Bioengineering, School of Engineering, The University of Tokyo, Tokyo, Japan.
authors.forename: Kazuhiko
authors.initials: K
authors.lastname: Ishihara
authors.affiliation: Department of Materials Engineering, School of Engineering, The University of Tokyo , Tokyo , Japan.
authors.forename: Weixin
authors.initials: W
authors.lastname: Chen
authors.affiliation: Department of Bioengineering, School of Engineering, The University of Tokyo , Tokyo , Japan.
authors.forename: Yihua
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Department of Materials Engineering, School of Engineering, The University of Tokyo , Tokyo , Japan.
authors.forename: Yuriko
authors.initials: Y
authors.lastname: Tsukamoto
authors.affiliation: Department of Materials Engineering, School of Engineering, The University of Tokyo , Tokyo , Japan.
authors.forename: Yuuki
authors.initials: Y
authors.lastname: Inoue
keywords: 30 Bio-inspired and biomedical materials; 600 Biomaterials, Nano materials; Nanomedicine molecular science; cytocompatibility; fluorescence property; in-cell device; surface modification; zwitterionic polymer