title: Aeromicrobium choanae sp. nov., an actinobacterium isolated from the choana of a garden warbler.
abstract: A Gram-stain-positive, non-spore-forming actinobacterium, strain 9H-4T, isolated from the choana of a garden warbler (Sylvia borin) was studied to examine its taxonomic position. On the basis of 16S rRNA gene sequence analysis it was shown that strain 9H-4T belongs to the genus Aeromicrobium with Aeromicrobium flavumTYLN1T (98.7 % similarity) and Aeromicrobium tamlenseSSW1-57T (98.4 %) as the nearest neighbours and forms a separate branch in a neighbour-joining phylogenetic tree based on 16S rRNA gene sequences. DNA-DNA hybridizations confirmed its novel species identity based on 39 and 46 % DNA-DNA relatedness with A. flavum DSM 19355T and A. tamlense DSM 19087T, respectively. The predominant menaquinone of strain 9H-4T was MK-9(H4). The peptidoglycan contained ll-diaminopimelic acid as the diagnostic diamino acid. The major fatty acids (C18 : 1ω9c, 10-methyl C18 : 0, C16 : 0, C18 : 0, C16 : 0 2-OH) were consistent with the fatty acid patterns reported for members of the genus Aeromicrobium. The DNA G+C content of strain 9H-4T was 70.8 mol%. The distinct genotypic, chemotaxonomic, physiological and biochemical characteristics support the classification of strain 9H-4T as a representative of a novel species of the genus Aeromicrobium, for which the name Aeromicrobium choanae sp. nov. is proposed. The type strain is 9H-4T (=ZIM B1021T=LMG 29165T=CCM 8650T).
authors.affiliation: Department of Biology, Faculty of Natural Sciences and Mathematics, University of Maribor, Maribor, Slovenia.
authors.forename: Polona
authors.initials: P
authors.lastname: Ber
authors.affiliation: BCCM/LMG Bacteria Collection, Laboratory of Microbiology, Faculty of Sciences, Ghent University, Ledeganckstraat 35, B-9000 Ghent, Belgium.
authors.forename: Stefanie Van
authors.initials: SV
authors.lastname: Trappen
authors.affiliation: BCCM/LMG Bacteria Collection, Laboratory of Microbiology, Faculty of Sciences, Ghent University, Ledeganckstraat 35, B-9000 Ghent, Belgium.
authors.forename: Peter
authors.initials: P
authors.lastname: Vandamme
authors.affiliation: Faculty of Chemistry and Chemical Engineering, University of Maribor, Maribor, Slovenia.|Department of Biology, Faculty of Natural Sciences and Mathematics, University of Maribor, Maribor, Slovenia.
authors.forename: Janja
authors.initials: J
authors.lastname: Trček
mesh_terms: D039903:Actinobacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D000818:Animals; D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D003960:Diaminopimelic Acid / Q000737:chemistry; D005227:Fatty Acids / Q000737:chemistry; D009055:Mouth / Q000382:microbiology; D009693:Nucleic Acid Hybridization; D010457:Peptidoglycan / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D020308:Songbirds / Q000382:microbiology*; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010457:Peptidoglycan; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; C073355:menaquinone 9; D003960:Diaminopimelic Acid