title: Extraction of Total DNA and RNA from Marine Filter Samples and Generation of a cDNA as Universal Template for Marker Gene Studies.
abstract: Microbial communities play an important role in marine ecosystem processes. Although the number of studies targeting marker genes such as the 16S rRNA gene has been increased in the last few years, the vast majority of marine diversity is rather unexplored. Moreover, most studies focused on the entire bacterial community and thus disregarded active microbial community players. Here, we describe a detailed protocol for the simultaneous extraction of DNA and RNA from marine water samples and for the generation of cDNA from the isolated RNA which can be used as a universal template in various marker gene studies.
authors.affiliation: Institut für Mikrobiologie und Genetik, Georg-August-Universität Göttingen, Grisebachstr. 8, 37077, Göttingen, Germany.
authors.forename: Dominik
authors.initials: D
authors.lastname: Schneider
authors.affiliation: Institut für Mikrobiologie und Genetik, Georg-August-Universität Göttingen, Grisebachstr. 8, 37077, Göttingen, Germany.
authors.forename: Franziska
authors.initials: F
authors.lastname: Wemheuer
authors.affiliation: Institut für Mikrobiologie und Genetik, Georg-August-Universität Göttingen, Grisebachstr. 8, 37077, Göttingen, Germany.
authors.forename: Birgit
authors.initials: B
authors.lastname: Pfeiffer
authors.affiliation: Institut für Mikrobiologie und Genetik, Georg-August-Universität Göttingen, Grisebachstr. 8, 37077, Göttingen, Germany. bwemheu@gwdg.de.
authors.forename: Bernd
authors.initials: B
authors.lastname: Wemheuer
mesh_terms: D059001:Aquatic Organisms / Q000235:genetics*; D044822:Biodiversity; D004247:DNA; D018076:DNA, Complementary; D005819:Genetic Markers; D008386:Marine Biology / Q000379:methods; D054892:Metagenome; D056186:Metagenomics / Q000379:methods; D012313:RNA
chemical_list: D018076:DNA, Complementary; D005819:Genetic Markers; D012313:RNA; D004247:DNA
keywords: Marker gene studies; Metagenomics; Metatranscriptomics; Microbial diversity; Microbial functions