title: Potential of DifferentColeus blumeiTissues for Rosmarinic Acid Production.
abstract: Rosmarinic acid is one of the main active components ofColeus blumeiand is known to have numerous health benefits. The pharmacological significance of rosmarinic acid and its production throughin vitroculture has been the subject of numerous studies. Here, the ability of different tissues to accumulate rosmarinic acid and sustainability in production over long cultivation have been tested. Calli, tumours, normal roots and hairy roots were established routinely by application of plant growth regulators or by transformation with agrobacteria. The differences among the established tumour lines were highly heterogeneous. Hairy root lines showed the highest mean growth rate and consistency in rosmarinic acid production. Although some tumour lines produced more rosmarinic acid than the hairy root lines, over a long cultivation period their productivity was unstable and decreased. Further, the effects of plant growth regulators on growth and rosmarinic acid accumulation were tested. 2,4-Dichlorophenoxyacetic acid significantly reduced tumour growth and rosmarinic acid production. 1-Naphthaleneacetic acid strongly stimulated hairy root growth whilst abscisic acid strongly enhanced rosmarinic acid production. Hairy roots cultured in an airlift bioreactor exhibited the highest potential for mass production of rosmarinic acid.
authors.affiliation: Department of Molecular Biology, Faculty of Science, University of Zagreb, Horvatovac 102a, HR-10000 Zagreb, Croatia; These authors contributed equally to this work.
authors.forename: Nataša
authors.initials: N
authors.lastname: Bauer
authors.affiliation: Department of Biology, University of Osijek, Cara Hadrijana 8/A, HR-31000 Osijek, Croatia; These authors contributed equally to this work.
authors.forename: Rosemary
authors.initials: R
authors.lastname: Vuković
authors.affiliation: Department of Botany, Faculty of Science, University of Zagreb, Marulićev trg 9a, HR-10000 Zagreb, Croatia.
authors.forename: Saša
authors.initials: S
authors.lastname: Likić
authors.affiliation: Department of Molecular Biology, Faculty of Science, University of Zagreb, Horvatovac 102a, HR-10000 Zagreb, Croatia.
authors.forename: Sibila
authors.initials: S
authors.lastname: Jelaska
keywords: 1-naphthaleneacetic acid; 2,4-dichlorophenoxyacetic acid; Coleus blumei; abscisic acid; bioreactor; callus; hairy root; root; rosmarinic acid; tumour