title: Therapeutic effect ofArctium lappainSchistosoma haematobiumassociated kidney disturbance: biochemical and molecular effects.
abstract: Schistosoma haematobium(S. haematobium) infection has been found to be strongly associated with bladder cancer, which necessitates for discover of a natural new therapeutic agent. The aim of this study was to evaluate the therapeutic effect ofArctium lappaseed extract inS. haematobiumassociated kidney disturbance. Forty male albino mice were used and divided into four equal groups; group 1 control includes non-infected healthy mice, groups 2, 3 and 4 subcutaneous infected withS. haematobiumcercariae. Groups 3 co-treated daily with oral dose ofA. lappaseed extract (300 mg/kg, bwt) for 15 days in the same time ofS. haematobiuminfection. Groups 4 post-treated daily for 15 days with oral dose ofA. lappaseed extract (300 mg/kg, bwt) after 15 days ofS. haematobiuminfection. The results obtained revealed thatS. haematobiumsignificantly decreased kidney weight and serum sodium, potassium and chloride, but increased urinary volume, urinary excretion of sodium, potassium and chloride, serum urea, creatinine and uric acid.Schistosoma haematobiumalso significantly decreased kidney superoxide dismutase, glutathione peroxidase and reduced glutathione levels while increased kidney lipid peroxidation level. Co- and post-treatment withA. lappaseed extract restore all the above parameters to approach the normal values. These results were supported with histopathological examinations. In conclusion,A. lappaseed extract has therapeutic effect in kidney disturbance caused byS. haematobiumwhere co-treatment ofA. lappaseed extract was more effective than post-treatment of the extract.
authors.affiliation: Department of Medical Physiology, Medical Research Division, National Research Centre, El-Buhouth Street, Dokki, Giza, 12622 Egypt ; Integrative Medicine Cluster, Advanced Medical and Dental Institute (AMDI), Universiti Sains Malaysia (USM), 13200 Penang, Malaysia.
authors.forename: Khaled M M
authors.initials: KM
authors.lastname: Koriem
authors.affiliation: Department of Medical Biochemistry, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, 43400 Serdang, Malaysia.
authors.forename: Zulzamri H
authors.initials: ZH
authors.lastname: Idris
authors.affiliation: Department of Medical Biochemistry, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, 43400 Serdang, Malaysia.
authors.forename: Hasniza F
authors.initials: HF
authors.lastname: Haron
authors.affiliation: Department of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 Serdang, Malaysia.
authors.forename: Nurulhuda A
authors.initials: NA
authors.lastname: Omar
authors.affiliation: Department of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 Serdang, Malaysia.
authors.forename: Halita S
authors.initials: HS
authors.lastname: Lazain
keywords: Arctium lappa; Asteraceae; DNA; Electrolytes; Mice; Schistosoma haematobium