title: A novel East African monopartite begomovirus-betasatellite complex that infects Vernonia amygdalina.
abstract: The complete genomes of a monopartite begomovirus (genus Begomovirus, family Geminiviridae) and an associated betasatellite found infecting Vernonia amygdalina Delile (family Compositae) in Uganda were cloned and sequenced. Begomoviruses isolated from two samples showed the highest nucleotide sequence identity (73.1% and 73.2%) to an isolate of the monopartite begomovirus tomato leaf curl Vietnam virus, and betasatellites from the same samples exhibited the highest nucleotide sequence identity (67.1% and 68.2%) to vernonia yellow vein Fujian betasatellite. Following the current taxonomic criteria for begomovirus species demarcation, the isolates sequenced here represent a novel begomovirus species. Based on symptoms observed in the field, we propose the name vernonia crinkle virus (VeCrV) for this novel begomovirus and vernonia crinkle betasatellite (VeCrB) for the associated betasatellite. This is the first report of a monopartite begomovirus-betasatellite complex from Uganda.
authors.affiliation: Instituto de Hortofruticultura Subtropical y Mediterránea "La Mayora", Universidad de Málaga - Consejo Superior de Investigaciones Científicas (IHSM-UMA-CSIC), Estación Experimental "La Mayora", 29750, Algarrobo-Costa, Málaga, Spain.
authors.forename: Happyness G
authors.initials: HG
authors.lastname: Mollel
authors.affiliation: Mikocheni Agricultural Research Institute, P.O. Box 6226, Dar es Salaam, Tanzania.
authors.forename: Joseph
authors.initials: J
authors.lastname: Ndunguru
authors.affiliation: Mikocheni Agricultural Research Institute, P.O. Box 6226, Dar es Salaam, Tanzania.
authors.forename: Peter
authors.initials: P
authors.lastname: Sseruwagi
authors.affiliation: National Crops Resources Research Institute, Namulonge, P.O. Box 7084, Kampala, Uganda.
authors.forename: Titus
authors.initials: T
authors.lastname: Alicai
authors.affiliation: Natural Resources Institute, University of Greenwich, Kent, ME4 4TB, UK.
authors.forename: John
authors.initials: J
authors.lastname: Colvin
authors.affiliation: Instituto de Hortofruticultura Subtropical y Mediterránea "La Mayora", Universidad de Málaga - Consejo Superior de Investigaciones Científicas (IHSM-UMA-CSIC), Estación Experimental "La Mayora", 29750, Algarrobo-Costa, Málaga, Spain.
authors.forename: Jesús
authors.initials: J
authors.lastname: Navas-Castillo
authors.affiliation: Instituto de Hortofruticultura Subtropical y Mediterránea "La Mayora", Universidad de Málaga - Consejo Superior de Investigaciones Científicas (IHSM-UMA-CSIC), Estación Experimental "La Mayora", 29750, Algarrobo-Costa, Málaga, Spain. efiallo@eelm.csic.es.
authors.forename: Elvira
authors.identifier: http://orcid.org/0000-0002-2460-1199
authors.initials: E
authors.lastname: Fiallo-Olivé
mesh_terms: D053839:Begomovirus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D004279:DNA, Viral / Q000235:genetics; D016679:Genome, Viral; D010802:Phylogeny; D010935:Plant Diseases / Q000821:virology*; D012526:Satellite Viruses / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D035741:Vernonia / Q000821:virology*
chemical_list: D004279:DNA, Viral