title: Generation of microgrooved silica nanotube membranes with sustained drug delivery and cell contact guidance ability by using a Teflon microfluidic chip.
abstract: Silica nanotubes have been extensively applied in the biomedical field. However, very little attention has been paid to the fabrication and application of micropatterned silica nanotubes. In the present study, microgrooved silica nanotube membranes were fabricatedin situby microgrooving silica-coated collagen hybrid fibril hydrogels in a Teflon microfluidic chip followed by calcination for removal of collagen fibrils. Scanning electron microscopy images showed that the resulting silica nanotube membranes displayed a typical microgroove/ridge surface topography with ∼50μm microgroove width and ∼120μm ridge width. They supported adsorption of bone morphogenetic protein 2 (BMP-2) and exhibited a sustained release behavior for BMP-2. After culturing with osteoblast MC3T3-E1 cells, they induced an enhanced osteoblast differentiation due to the release of biologically active BMP-2 and a strong contact guidance ability to directly align and elongate osteoblasts due to the presence of microgrooved surface topography, indicating their potential application as a multi-functional cell-supporting matrix for tissue generation.
authors.affiliation: JSPS Research Fellow, 8 Ichibancho, Chiyoda-ku, Tokyo 102-8472, Japan; Biomaterials Unit, National Institute for Materials Science, Sengen, Tsukuba, 305-0047, Japan.
authors.forename: Song
authors.initials: S
authors.lastname: Chen
authors.affiliation: WPI Advanced Institute for Materials Research, Tohoku University, Katahira 2-1-1, Aobaku, Sendai 980-8577, Japan.
authors.forename: Xuetao
authors.initials: X
authors.lastname: Shi
authors.affiliation: Biomaterials Unit, National Institute for Materials Science, Sengen, Tsukuba, 305-0047, Japan; Department of Medical Physics, Anna University, Chennai 600-025, India.
authors.forename: Shanmugavel
authors.initials: S
authors.lastname: Chinnathambi
authors.affiliation: WPI Advanced Institute for Materials Research, Tohoku University, Katahira 2-1-1, Aobaku, Sendai 980-8577, Japan; Department of Chemistry, Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, People's Republic of China.
authors.forename: Hongkai
authors.initials: H
authors.lastname: Wu
authors.affiliation: Biomaterials Unit, National Institute for Materials Science, Sengen, Tsukuba, 305-0047, Japan; Nanotechnology Innovation Station, National Institute for Materials Science, 1-2-1 Sengen, Tsukuba, Ibaraki 305-0047, Japan; Graduate School of Life Science, Hokkaido University, N10W8, Kita-ku, Sapporo 060-0812, Japan.
authors.forename: Nobutaka
authors.initials: N
authors.lastname: Hanagata
keywords: 10.1; Teflon chip; cell alignment and elongation; drug delivery; microgroove; silica nanotube