title: Proteomic profiling of NCI-60 extracellular vesicles uncovers common protein cargo and cancer type-specific biomarkers.
abstract: Packed with biological information, extracellular vesicles (EVs) offer exciting promise for biomarker discovery and applications in therapeutics and non-invasive diagnostics. Currently, our understanding of EV contents is confined by the limited cells from which vesicles have been characterized utilizing the same enrichment method. Using sixty cell lines from the National Cancer Institute (NCI-60), here we provide the largest proteomic profile of EVs in a single study, identifying 6,071 proteins with 213 common to all isolates. Proteins included established EV markers, and vesicular trafficking proteins such as Rab GTPases and tetraspanins. Differentially-expressed proteins offer potential for cancer diagnosis and prognosis. Network analysis of vesicle quantity and proteomes identified EV components associated with vesicle secretion, including CD81, CD63, syntenin-1, VAMP3, Rab GTPases, and integrins. Integration of vesicle proteomes with whole-cell molecular profiles revealed similarities, suggesting EVs provide a reliable reflection of their progenitor cell content, and are therefore excellent indicators of disease.
authors.affiliation: Department of Biomedical Sciences, Florida State University College of Medicine, Tallahassee, FL, 32306, USA.
authors.forename: Stephanie N
authors.initials: SN
authors.lastname: Hurwitz
authors.affiliation: Department of Biomedical Sciences, Florida State University College of Medicine, Tallahassee, FL, 32306, USA.
authors.forename: Mark A
authors.initials: MA
authors.lastname: Rider
authors.affiliation: Department of Biomedical Sciences, Florida State University College of Medicine, Tallahassee, FL, 32306, USA.
authors.forename: Joseph L
authors.initials: JL
authors.lastname: Bundy
authors.affiliation: Department of Biomedical Sciences, Florida State University College of Medicine, Tallahassee, FL, 32306, USA.
authors.forename: Xia
authors.initials: X
authors.lastname: Liu
authors.affiliation: Department of Biomedical Sciences, Florida State University College of Medicine, Tallahassee, FL, 32306, USA.
authors.forename: Rakesh K
authors.initials: RK
authors.lastname: Singh
authors.affiliation: Department of Biomedical Sciences, Florida State University College of Medicine, Tallahassee, FL, 32306, USA.
authors.forename: David G
authors.initials: DG
authors.lastname: Meckes
mesh_terms: D014408:Biomarkers, Tumor / Q000032:analysis*; D045744:Cell Line, Tumor; D000067128:Extracellular Vesicles / Q000737:chemistry*; D006801:Humans; D040901:Proteomics / Q000379:methods*
chemical_list: D014408:Biomarkers, Tumor
keywords: biomarkers; co-inertia; exosomes; microvesicles; proteomics