title: Profiling and identification of new proteins involved in brain ischemia using MALDI-imaging-mass-spectrometry.
abstract: The identification of proteins involved in brain ischemia might allow the discovery of putative biomarkers or therapeutic targets for ischemic stroke. Our aim is to study the distribution of proteins within mouse brain after an ischemic insult using MALDI imaging-mass-spectrometry and to identify relevant proteins involved in brain damage. We occluded the middle cerebral artery of C57BL/6J mice. Brain slices were analyzed by MALDI-TOF and infarct (IC) and contralateral (CL) regions were compared using ClinProTools. The ion distribution maps of relevant m/z values were obtained by FlexImagin3.0. Protein identification was conducted through a bottom-up approach consisting on complementary sample fractionation methods. Some identifications were confirmed by immunohistochemistry and western blot. We identified 102 m/z values with different abundances between IC and CL (p<0.05), from which 21 m/z peaks were selected as more relevant. Thirteen of them were found increased in the infarct region and 4 m/z values showed AUC>90% between IC and CL. Identification analyses confirmed altered expressions of ATP5i, COX6C and UMP-CMP kinase in IC compared to CL.
  
  
  
  Using MALDI-IMS we identified for the first time new proteins that might be involved in brain ischemia representing potential diagnostic biomarkers or target molecules for neurological recovery.
authors.affiliation: Universitat Autònoma de Barcelona, Neurovascular Research Laboratory, Institut de Recerca Vall d'Hebron, Barcelona, Spain. Electronic address: llombs@hotmail.com.
authors.forename: Víctor
authors.initials: V
authors.lastname: Llombart
authors.affiliation: Instituto Multidisciplinario de Biología Celular (IMBICE), CONICET, Argentina; Universitat Autònoma de Barcelona, Structural Biology and Proteomics Service, Barcelona, Spain. Electronic address: sebatrejo@gmail.com.
authors.forename: Sebastián Alejandro
authors.initials: SA
authors.lastname: Trejo
authors.affiliation: Universitat Autònoma de Barcelona, Structural Biology and Proteomics Service, Barcelona, Spain. Electronic address: Silvia.Bronsoms@uab.cat.
authors.forename: Sílvia
authors.initials: S
authors.lastname: Bronsoms
authors.affiliation: Universitat Autònoma de Barcelona, Neurovascular Research Laboratory, Institut de Recerca Vall d'Hebron, Barcelona, Spain. Electronic address: annamorancho@gmail.com.
authors.forename: Anna
authors.initials: A
authors.lastname: Morancho
authors.affiliation: Universitat Autònoma de Barcelona, Neurovascular Research Laboratory, Institut de Recerca Vall d'Hebron, Barcelona, Spain. Electronic address: mafeifeivhir@hotmail.com.
authors.forename: Ma
authors.initials: M
authors.lastname: Feifei
authors.affiliation: Universitat Autònoma de Barcelona, Neurovascular Research Laboratory, Institut de Recerca Vall d'Hebron, Barcelona, Spain. Electronic address: juliafaura10@gmail.com.
authors.forename: Júlia
authors.initials: J
authors.lastname: Faura
authors.affiliation: Universitat Autònoma de Barcelona, Neurovascular Research Laboratory, Institut de Recerca Vall d'Hebron, Barcelona, Spain. Electronic address: teresa.garcib@gmail.com.
authors.forename: Teresa
authors.initials: T
authors.lastname: García-Berrocoso
authors.affiliation: Universitat Autònoma de Barcelona, Neurovascular Research Laboratory, Institut de Recerca Vall d'Hebron, Barcelona, Spain. Electronic address: alba.simats@vhir.org.
authors.forename: Alba
authors.initials: A
authors.lastname: Simats
authors.affiliation: Universitat Autònoma de Barcelona, Neurovascular Research Laboratory, Institut de Recerca Vall d'Hebron, Barcelona, Spain. Electronic address: anna.rosell@gmail.com.
authors.forename: Anna
authors.initials: A
authors.lastname: Rosell
authors.affiliation: Proteomics Laboratory, Vall d'Hebrón Institute of Oncology, Barcelona, Spain. Electronic address: fcanals@vhio.net.
authors.forename: Francesc
authors.initials: F
authors.lastname: Canals
authors.affiliation: Universitat Autònoma de Barcelona, Neurovascular Research Laboratory, Institut de Recerca Vall d'Hebron, Barcelona, Spain. Electronic address: marguillamon@gmail.com.
authors.forename: Mar
authors.initials: M
authors.lastname: Hernández-Guillamón
authors.affiliation: Universitat Autònoma de Barcelona, Neurovascular Research Laboratory, Institut de Recerca Vall d'Hebron, Barcelona, Spain. Electronic address: montaner.villalonga@gmail.com.
authors.forename: Joan
authors.initials: J
authors.lastname: Montaner
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000032:analysis; D001923:Brain Chemistry; D002545:Brain Ischemia / Q000000981:diagnostic imaging* / Q000378:metabolism; D003952:Diagnostic Imaging / Q000379:methods*; D020869:Gene Expression Profiling; D051379:Mice; D011506:Proteins / Q000032:analysis* / Q000378:metabolism; D040901:Proteomics / Q000379:methods*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods; D020521:Stroke / Q000000981:diagnostic imaging
chemical_list: D015415:Biomarkers; D011506:Proteins
keywords: Biomarkers; Cerebral ischemia; Ischemic stroke; MALDI-imaging; tMCAO