title: Chiral separation of benzothiazole derivatives of amino acids using capillary zone electrophoresis.
abstract: A method for the separation of enantiomers of leucine and phenylalanine benzothiazole derivatives as potential antimicrobial agents was developed using capillary zone electrophoresis with a dual cyclodextrin (CD) system. The best resolution of enantiomers was achieved in 100 mmol/L phosphate background electrolyte (pH 3.5) with the dual CD system consisting of 10 mmol/L of β-CD with 10 mmol/L of 2-hydroxypropyl-β-cyclodextrin for leucine derivative and 10 mmol/L of 2-hydroxypropyl-γ-cyclodextrin for phenylalanine derivative, respectively. Under the optimal conditions, the highest enantioresolution of 1.25 was achieved in a noncoated-fused silica capillary at 17°C and 24 kV applied voltage.
authors.affiliation: Department of Analytical Chemistry, Faculty of Chemical Technology, University of Pardubice, Pardubice, Czech Republic.
authors.forename: Zuzana
authors.initials: Z
authors.lastname: Nováková
authors.affiliation: Institute of Organic Chemistry and Technology, Faculty of Chemical Technology, University of Pardubice, Pardubice, Czech Republic.
authors.forename: Vladimír
authors.initials: V
authors.lastname: Pejchal
authors.affiliation: Department of Analytical Chemistry, Faculty of Chemical Technology, University of Pardubice, Pardubice, Czech Republic.
authors.forename: Jan
authors.initials: J
authors.lastname: Fischer
authors.affiliation: Department of Analytical Chemistry, Faculty of Chemical Technology, University of Pardubice, Pardubice, Czech Republic.
authors.forename: Petr
authors.initials: P
authors.lastname: Česla
mesh_terms: D000596:Amino Acids / Q000737:chemistry* / Q000378:metabolism; D052160:Benzothiazoles / Q000302:isolation & purification*; D002623:Chemistry Techniques, Analytical / Q000379:methods*; D003505:Cyclodextrins / Q000737:chemistry; D019075:Electrophoresis, Capillary; D013237:Stereoisomerism
chemical_list: D000596:Amino Acids; D052160:Benzothiazoles; D003505:Cyclodextrins
keywords: amino acids; benzothiazole derivatives; capillary zone electrophoresis; dual cyclodextrin systems; polydimethylacrylamide-coated capillaries