title: Scintillation imaging of tritium radioactivity distribution during tritiated thymidine uptake by PC12 cells using a melt-on scintillator.
abstract: A scintillation image of tritium fixed in a melt-on scintillator was obtained using a charged-coupled device (CCD) imager, and a linear relationship was observed between the intensity of the scintillation image and the radioactivity of tritium. In a [3H]thymidine uptake experiment, a linear correlation between the intensity of the CCD image and the dilution ratio of cells was confirmed. Scintillation imaging has the potential for use in direct observation of tritium radioactivity distribution.
authors.affiliation: Institute of Biomedical Science, Tokushima University, 3-18-15 Kuramoto-cho, Tokushima 770-8503, Japan; Graduate School of Pharmaceutical Sciences, Tokushima University, 1-78 Shomachi, Tokushima 770-8505, Japan. Electronic address: irikura@tokushima-u.ac.jp.
authors.forename: Namiko
authors.initials: N
authors.lastname: Irikura
authors.affiliation: Radioisotope Research Center, Tokushima University, 3-18-15 Kuramoto-cho, Tokushima 770-8503, Japan.
authors.forename: Hirokazu
authors.initials: H
authors.lastname: Miyoshi
authors.affiliation: Graduate School of Pharmaceutical Sciences, Tokushima University, 1-78 Shomachi, Tokushima 770-8505, Japan; Institute for Genome Research, Tokushima University, 3-18-15 Kuramoto-cho, Tokushima 770-8503, Japan.
authors.forename: Yasuo
authors.initials: Y
authors.lastname: Shinohara
mesh_terms: D000818:Animals; D001610:Beta Particles; D016716:PC12 Cells; D019275:Radiopharmaceuticals / Q000493:pharmacokinetics*; D051381:Rats; D012588:Scintillation Counting / Q000295:instrumentation* / Q000379:methods; D013936:Thymidine / Q000493:pharmacokinetics*; D014316:Tritium / Q000493:pharmacokinetics*
chemical_list: D019275:Radiopharmaceuticals; D014316:Tritium; D013936:Thymidine
keywords: CCD; Imaging without disposable material; Melt-on scintillator; Scintillation; Tritium