title: A Method for Determining the Content of Glycoproteins in Biological Samples.
abstract: The glycoprotein purified from the mycelium extract ofTremella fuciformiswas marked with iodine through the iodine substitution reaction. The content of iodine, which is indicative of the amount of the marked tremella glycoprotein (ITG), was detected with Inductively coupled plasma mass spectrometry (ICP-MS). The method was found to be stable, sensitive, and accurate at detecting the content of iodine-substituted glycoprotein, and was used in the quantitative analysis of biological samples, including blood and organs. Different biological samples were collected from rats after oral administration of ITG, and were tested for iodine content by ICP-MS to calculate the amount of ITG in the samples. The results suggested that ICP-MS is a sensitive, stable, and accurate method for detection of iodinated glycoproteins in blood and organs.
authors.affiliation: Jilin Key Laboratory of Macromolecules in Chinese Drugs, Changchun University of Chinese Traditional Medicine, Changchun 130117, Jilin, China. gaoyang-1979@hotmail.com.
authors.forename: Yang
authors.initials: Y
authors.lastname: Gao
authors.affiliation: Jilin Key Laboratory of Macromolecules in Chinese Drugs, Changchun University of Chinese Traditional Medicine, Changchun 130117, Jilin, China. czxuduoduo@163.com.
authors.forename: Duoduo
authors.initials: D
authors.lastname: Xu
authors.affiliation: Jilin Key Laboratory of Macromolecules in Chinese Drugs, Changchun University of Chinese Traditional Medicine, Changchun 130117, Jilin, China. 18311164062@163.com.
authors.forename: Hongyue
authors.initials: H
authors.lastname: Li
authors.affiliation: Jilin Institute For Drug Control, Changchun 130033, Jilin, China. vivienyang22@sina.com.
authors.forename: Xianling
authors.initials: X
authors.lastname: Yang
authors.affiliation: Jilin Key Laboratory of Macromolecules in Chinese Drugs, Changchun University of Chinese Traditional Medicine, Changchun 130117, Jilin, China. cc_wmx@163.com.
authors.forename: Mingxing
authors.initials: M
authors.lastname: Wang
authors.affiliation: Jilin Key Laboratory of Macromolecules in Chinese Drugs, Changchun University of Chinese Traditional Medicine, Changchun 130117, Jilin, China. gaoqipin@sina.com.
authors.forename: Qipin
authors.initials: Q
authors.lastname: Gao
mesh_terms: D000818:Animals; D001487:Basidiomycota / Q000737:chemistry*; D002478:Cells, Cultured; D002850:Chromatography, Gel; D006023:Glycoproteins / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification / Q000493:pharmacokinetics; D007455:Iodine / Q000032:analysis; D057230:Limit of Detection; D051381:Rats; D015203:Reproducibility of Results; D014018:Tissue Distribution
chemical_list: D006023:Glycoproteins; D007455:Iodine
keywords: ICP-MS; biological sample; glycoprotein; iodine substitution; quantification