title: Regulation of sugar transporter activity for antibacterial defense in Arabidopsis.
abstract: Microbial pathogens strategically acquire metabolites from their hosts during infection. Here we show that the host can intervene to prevent such metabolite loss to pathogens. Phosphorylation-dependent regulation of sugar transport protein 13 (STP13) is required for antibacterial defense in the plant Arabidopsis thaliana STP13 physically associates with the flagellin receptor flagellin-sensitive 2 (FLS2) and its co-receptor BRASSINOSTEROID INSENSITIVE 1-associated receptor kinase 1 (BAK1). BAK1 phosphorylates STP13 at threonine 485, which enhances its monosaccharide uptake activity to compete with bacteria for extracellular sugars. Limiting the availability of extracellular sugar deprives bacteria of an energy source and restricts virulence factor delivery. Our results reveal that control of sugar uptake, managed by regulation of a host sugar transporter, is a defense strategy deployed against microbial infection. Competition for sugar thus shapes host-pathogen interactions.
authors.affiliation: Graduate School of Agriculture, Kyoto University, Kyoto, Japan. kohjiyamada226@gmail.com ytakano@kais.kyoto-u.ac.jp.|Graduate School of Bioscience and Bioindustry, Tokushima University, Tokushima, Japan.
authors.forename: Kohji
authors.initials: K
authors.lastname: Yamada
authors.affiliation: Graduate School of Biological Sciences, Nara Institute of Science and Technology, Ikoma, Japan.|Japan Science and Technology Agency (JST), Precursory Research for Embryonic Science and Technology (PRESTO), Kawaguchi, Japan.
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Saijo
authors.affiliation: RIKEN Center for Sustainable Resource Science, Yokohama, Japan.
authors.forename: Hirofumi
authors.initials: H
authors.lastname: Nakagami
authors.affiliation: Graduate School of Agriculture, Kyoto University, Kyoto, Japan. kohjiyamada226@gmail.com ytakano@kais.kyoto-u.ac.jp.
authors.forename: Yoshitaka
authors.initials: Y
authors.lastname: Takano
mesh_terms: D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000378:metabolism* / Q000382:microbiology*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D001692:Biological Transport; D004734:Energy Metabolism; D005408:Flagellin / Q000378:metabolism; D054884:Host-Pathogen Interactions; D009004:Monosaccharide Transport Proteins / Q000235:genetics / Q000378:metabolism; D009005:Monosaccharides / Q000378:metabolism*; D010766:Phosphorylation; D010935:Plant Diseases / Q000382:microbiology*; D011494:Protein Kinases / Q000378:metabolism; D011499:Protein Processing, Post-Translational; D044224:Pseudomonas syringae / Q000378:metabolism / Q000472:pathogenicity; D027981:Symporters / Q000235:genetics / Q000378:metabolism*; D013912:Threonine / Q000235:genetics / Q000378:metabolism; D037521:Virulence Factors / Q000378:metabolism
chemical_list: D029681:Arabidopsis Proteins; D009004:Monosaccharide Transport Proteins; D009005:Monosaccharides; C000605031:STP1 protein, Arabidopsis; C510622:STP13 protein, Arabidopsis; D027981:Symporters; D037521:Virulence Factors; D005408:Flagellin; D013912:Threonine; D011494:Protein Kinases; C411853:FLS2 protein, Arabidopsis; C108270:BRI1 protein, Arabidopsis