title: Comparative Transcriptomic Analyses of Differentially Expressed Genes in Transgenic Melatonin Biosynthesis OvineHIOMTGene in Switchgrass.
abstract: Melatonin serves pleiotropic functions in prompting plant growth and resistance to various stresses. The accurate biosynthetic pathway of melatonin remains elusive in plant species, while theN-acetyltransferase andO-methyltransferase were considered to be the last two key enzymes during its biosynthesis. To investigate the biosynthesis and metabolic pathway of melatonin in plants, the RNA-seq profile of overexpression of the ovineHIOMTwas analyzed and compared with the previous transcriptome of transgenicoAANATgene in switchgrass, a model plant for cellulosic ethanol production. A total of 946, 405, and 807 differentially expressed unigenes were observed inAANATvs. control,HIOMTvs. control, andAANATvs.HIOMT, respectively. Two hundred and seventy-five upregulated and 130 downregulated unigenes were detected in transgenicoHIOMTline comparing with control, including the significantly upregulated (F-box/kelch-repeat protein, zinc finger BED domain-containing protein-3) genes, which were potentially correlated with enhanced phenotypes of shoot, stem and root growth in transgenicoHIOMTswitchgrass. Several stress resistant related genes (SPX domain-containing membrane protein, copper transporter 1, late blight resistance protein homolog R1A-6 OS etc.) were specifically and significantly upregulated in transgenicoHIOMTonly, while metabolism-related genes (phenylalanine-4-hydroxylase, tyrosine decarboxylase 1, protein disulfide-isomerase and galactinol synthase 2 etc.) were significantly upregulated in transgenicoAANATonly. These results provide new sights into the biosynthetic and physiological functional networks of melatonin in plants.
authors.affiliation: College of Animal Science and Technology, China Agricultural University Beijing, China.
authors.forename: Shan
authors.initials: S
authors.lastname: Yuan
authors.affiliation: College of Animal Science and Technology, China Agricultural University Beijing, China.
authors.forename: Cong
authors.initials: C
authors.lastname: Guan
authors.affiliation: College of Animal Science and Technology, China Agricultural University Beijing, China.
authors.forename: Sijia
authors.initials: S
authors.lastname: Liu
authors.affiliation: College of Animal Science and Technology, China Agricultural UniversityBeijing, China; College of Agriculture, China Agricultural UniversityBeijing, China.
authors.forename: Yanhua
authors.initials: Y
authors.lastname: Huang
authors.affiliation: College of Animal Science and Technology, China Agricultural University Beijing, China.
authors.forename: Danyang
authors.initials: D
authors.lastname: Tian
authors.affiliation: College of Animal Science and Technology, China Agricultural University Beijing, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Cui
authors.affiliation: College of Animal Science and Technology, China Agricultural UniversityBeijing, China; Beijing Key Laboratory for Grassland Science, China Agricultural UniversityBeijing, China; National Energy R&D Center for BiomassBeijing, China.
authors.forename: Yunwei
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: College of Animal Science and Technology, China Agricultural UniversityBeijing, China; Beijing Sure Academy of BiosciencesBeijing, China.
authors.forename: Fuyu
authors.initials: F
authors.lastname: Yang
keywords: RNA-seq; melatonin; oHIOMT; switchgrass; transgene