title: Long-term straw returning affects Nitrospira-like nitrite oxidizing bacterial community in a rapeseed-rice rotation soil.
abstract: Nitrospira are the most widespread and well known nitrite-oxidizing bacteria (NOB) and putatively key nitrite-oxidizers in acidic ecosystems. Nevertheless, their ecology in agriculture soils has not been well studied. To understand the impact of straw incorporation on soil Nitrospira-like bacterial community, a cloned library analysis of the nitrite oxidoreductase gene-nxrB was performed for a long-term rapeseed-rice rotation system. In this study, most members of the Nitrospira-like NOB in the paddy soils from the Wuxue field experiment station were phylogenetically related with Nitrospira lineages II. The Shannon diversity index possessed a decrease trend in the straw applied soils. The relative abundances of 16 OTUs (accounting 72% of the total OTUs, including 11 unique OTUs and 5 shared OTUs) were different between in the straw applied and control soils. These data suggested a selection effect from the long-term straw fertilization. Canonical correspondence analysis data showed that a centralized group of Nitrospira-like NOB OTUs in the community was partly explained by the soil ammonium, nitrate, available phosphorus, and the available potassium. This could suggest that straw fertilization led to the soil Nitrospira-like NOB community shift, which was correlated with the change of available nutrients in the bulk soil.
authors.affiliation: State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, China.|Key Laboratory of Arable Land Conservation (Middle and Lower Reaches of Yangtze River), Ministry of Agriculture, College of Resources and Environment, Huazhong Agricultural University, Wuhan, China.
authors.forename: Xuesong
authors.initials: X
authors.lastname: Luo
authors.affiliation: State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, China.
authors.forename: Shun
authors.initials: S
authors.lastname: Han
authors.affiliation: State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, China.
authors.forename: Songsong
authors.initials: S
authors.lastname: Lai
authors.affiliation: State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, China.|Key Laboratory of Arable Land Conservation (Middle and Lower Reaches of Yangtze River), Ministry of Agriculture, College of Resources and Environment, Huazhong Agricultural University, Wuhan, China.
authors.forename: Qiaoyun
authors.initials: Q
authors.lastname: Huang
authors.affiliation: State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, China.
authors.forename: Wenli
authors.initials: W
authors.lastname: Chen
mesh_terms: D000383:Agriculture / Q000379:methods; D001419:Bacteria / Q000235:genetics / Q000378:metabolism*; D029741:Brassica rapa; D018556:Crops, Agricultural; D059013:Microbial Consortia / Q000502:physiology*; D058465:Nitrification; D009573:Nitrites / Q000378:metabolism*; D012275:Oryza; D010084:Oxidation-Reduction; D018547:Plant Stems; D012987:Soil / Q000737:chemistry; D012988:Soil Microbiology
chemical_list: D009573:Nitrites; D012987:Soil
keywords: Nitrospira-like nitrite oxidizing bacteria; NxrB; clone library; diversity; straw incorporation