title: Characterization and control of symbioticBacillus cereusisolated from the mid gut ofAnopheles subpictusGrassi.
abstract: An investigation was done to find out the role of gut bacterium on the larval development and survival ofAnopheles subpictus, the vector responsible for the transmission of malaria.An. subpictusmosquitoes breed exclusively in stagnant water, including shrimp/fish ponds with high nutrient level. One bacterial strain (CX2) was isolated from the midgut of late third instar of mosquito larvae. The bacterial isolate was sensitive to recommended doses of tetracyclin (30 µg/disc), doxycycline (30 µg/disc), kanamycin (30 µg/disc), gentamycin (10 µg/disc), streptomycin (10 µg/disc), vancomycin (30 µg/disc), ofloxacin (5 µg/disc), levofloxacin (5 µg/disc), gatifloxacin (10 µg/disc), but resistant to ampicillin (10 µg/disc).The larvae which were fed with the mixture of two antibiotics tetracylin (30 µg/disc) and doxycyclin (30 µg/disc) (1:1) could not survive in rice-field water. In the control experiments without any antibiotic treatment, 95-100 % survival and 95 % adult emergence were observed. The study indicates that the elimination of gut bacteria suppressed larval growth. Phylogenetic analysis of the 16S rRNA gene sequence was also done. Based on the morphological, biochemical, FAME analysis and phylogenetic analysis, the bacterial isolate CX2 was identified asBacillus cereus. Poly acrylamide gel electrophoresis analysis revealed that the isolate showed discrete bands ranging from 24.272 to 60.049 kDa proteins. Water extract and methanol extract ofTamarindus indicashowed inhibitory effect againstB. cereus.
authors.affiliation: Microbiology and Parasitology Research Laboratory, Department of Zoology, The University of Burdwan, Burdwan, West Bengal 713 104 India.
authors.forename: Priyanka
authors.initials: P
authors.lastname: Mukhopadhyay
authors.affiliation: Microbiology and Parasitology Research Laboratory, Department of Zoology, The University of Burdwan, Burdwan, West Bengal 713 104 India.
authors.forename: Soumendranath
authors.initials: S
authors.lastname: Chatterjee
keywords: Anopheles subpictus; Bacillus cereus; Biocontrol; Phylogenetic analysis; Symbiotic association; Tamarindus indica