title: Facile fabrication of hydrophilic PAA-Ti/TiO2nanocomposite for selective enrichment and detection of phosphopeptides from complex biological samples.
abstract: Highly selective enrichment of trace phosphorylated proteins or peptides from complex biological samples is of profound significance for the discovery of disease biomarkers in biological systems. In this study, a novel affinity material has been synthesized to improve the enrichment specificity for phosphopeptides by using PAAS as coupling molecule. In the resulting materials, highly abundant titanium is available for selective enrichment of phosphopeptides, with plenty of carboxylate groups that can inhibit nonspecific adsorption. The enrichment results demonstrated that the hydrophilic PAA-Ti/TiO2composite possesses excellent selectivity for phosphopeptides even at a very low molar ratio of phosphopeptides/non-phosphopeptides (1:1000), extreme sensitivity (the detection limit was at the fmol level), and high recovery of phosphopeptides (as high as 78%). Moreover, the as-prepared nanocomposite provides effective enrichment of phosphopeptides from real samples (mouse liver), showing great potential in the detection of low-abundance phosphopeptides in biological samples.
authors.affiliation: Department of Chemistry, Dalian University of Technology, Dalian, 116023, China.
authors.forename: Xiao-Na
authors.initials: XN
authors.lastname: Wei
authors.affiliation: Department of Chemistry, Dalian University of Technology, Dalian, 116023, China. Electronic address: hlwang@dlut.edu.cn.
authors.forename: Hui-Long
authors.initials: HL
authors.lastname: Wang
mesh_terms: D000327:Adsorption; D000818:Animals; D008099:Liver / Q000737:chemistry; D051379:Mice; D053761:Nanocomposites / Q000737:chemistry*; D010748:Phosphopeptides / Q000032:analysis*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014025:Titanium / Q000737:chemistry*
chemical_list: D010748:Phosphopeptides; C009495:titanium dioxide; D014025:Titanium
keywords: Affinity material; Mass spectrometry; Nanoparticle; PAA-Ti/TiO(2) composite; Phosphopeptide enrichment