title: Upconversion nanoparticles grafted molybdenum disulfide nanosheets platform for microcystin-LR sensing.
abstract: Water safety is one of the most pervasive problems afflicting people throughout the world. Microcystin-LR (MC-LR), a representative toxin released by cyanobacteria, poses an increasing and serious threat to water safety. In order to develop facile, specific and sensitive detection methods for MC-LR, we fabricated an ultrasensitive fluorescence aptasensor using the enhanced fluorescence of UCNP and the effective quenching ability, high affinity toward single strand DNA (ssDNA) of MoS2(termed as FAUM). This assay specifically determined MC-LR in the linear range of 0.01-50ng/ml with a limit of detection (LOD) of 0.002ng/ml. The real water sample results indicated that this FAUM assay owns well enough reliability and feasibility to allow the determination of MC-LR. This aptamer-based method might be a promising strategy for a variety of sensing applications.
authors.affiliation: State Key Laboratory of Food Science and Technology, Synergetic Innovation Center of Food Safety and Nutrition, School of Food Science and Technology, Jiangnan University, Wuxi 214122, China.
authors.forename: Jiajia
authors.initials: J
authors.lastname: Lv
authors.affiliation: State Key Laboratory of Food Science and Technology, Synergetic Innovation Center of Food Safety and Nutrition, School of Food Science and Technology, Jiangnan University, Wuxi 214122, China.
authors.forename: Sen
authors.initials: S
authors.lastname: Zhao
authors.affiliation: State Key Laboratory of Food Science and Technology, Synergetic Innovation Center of Food Safety and Nutrition, School of Food Science and Technology, Jiangnan University, Wuxi 214122, China. Electronic address: wusj@jiangnan.edu.cn.
authors.forename: Shijia
authors.initials: S
authors.lastname: Wu
authors.affiliation: State Key Laboratory of Food Science and Technology, Synergetic Innovation Center of Food Safety and Nutrition, School of Food Science and Technology, Jiangnan University, Wuxi 214122, China; School of Food Science and Technology, Shihezi University, Shihezi 832003, China. Electronic address: wangzp@jiangnan.edu.cn.
authors.forename: Zhouping
authors.initials: Z
authors.lastname: Wang
mesh_terms: D052157:Aptamers, Nucleotide / Q000737:chemistry*; D015374:Biosensing Techniques / Q000379:methods*; D000458:Cyanobacteria / Q000737:chemistry; D004220:Disulfides / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry; D005459:Fluorides / Q000737:chemistry*; D057230:Limit of Detection; D008387:Marine Toxins; D052998:Microcystins / Q000032:analysis*; D008982:Molybdenum / Q000737:chemistry*; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D015203:Reproducibility of Results; D013050:Spectrometry, Fluorescence / Q000379:methods; D014867:Water / Q000032:analysis; D014874:Water Pollutants, Chemical / Q000032:analysis*; D015018:Ytterbium / Q000737:chemistry; D015019:Yttrium / Q000737:chemistry*
chemical_list: D052157:Aptamers, Nucleotide; D004220:Disulfides; D005456:Fluorescent Dyes; D008387:Marine Toxins; D052998:Microcystins; D014874:Water Pollutants, Chemical; C525362:sodium yttriumtetrafluoride; D014867:Water; D015019:Yttrium; D008982:Molybdenum; C057862:cyanoginosin LR; D015018:Ytterbium; D005459:Fluorides; C082964:molybdenum disulfide
keywords: Aptamer; MC-LR; MoS(2); Upconversion nanoparticles