title: Obesity‑associated miR‑148a is regulated by cytokines and adipokines via a transcriptional mechanism.
abstract: Our previous study revealed that miR‑148a, a cyclic adenosine monophosphate‑response element binding protein‑modulated microRNA that promotes adipocyte differentiation by inhibiting Wnt1, is a biomarker of obesity in human subjects and a mouse model. The present study investigated the expression of miR‑148a in human adipose tissue‑derived mesenchymal stem cells (hMSCs‑Ad) in response to inflammatory cytokines and adipokines to clarify its underlying mechanism. miR‑148a expression was detected using reverse transcription‑quantitative polymerase chain reaction analysis and its promoter activity was detected with a luciferase assay. miR‑148a expression levels decreased when differentiated hMSCs‑Ad were exposed to inflammatory cytokines or adipokines, which suggested that miR‑148a may be important in adipocyte metabolism and inflammation. Furthermore, the promoter activity of miR‑148a decreased following treatment of cells with inflammatory cytokines or adipokines. The results of the present study indicated a novel role of miR‑148a in adipocyte inflammation; therefore, miR‑148a may be involved in obesity complications via its own underlying transcriptional mechanism.
authors.affiliation: Department of Children Health Care, Nanjing Maternity and Child Health Care Hospital Affiliated to Nanjing Medical University, Nanjing, Jiangsu 210029, P.R. China.
authors.forename: Chunmei
authors.initials: C
authors.lastname: Shi
authors.affiliation: Department of Children Health Care, Nanjing Maternity and Child Health Care Hospital Affiliated to Nanjing Medical University, Nanjing, Jiangsu 210029, P.R. China.
authors.forename: Lingxia
authors.initials: L
authors.lastname: Pang
authors.affiliation: Department of Children Health Care, Nanjing Maternity and Child Health Care Hospital Affiliated to Nanjing Medical University, Nanjing, Jiangsu 210029, P.R. China.
authors.forename: Chenbo
authors.initials: C
authors.lastname: Ji
authors.affiliation: Department of Pediatrics, The Third Affiliated Hospital of Xinxiang Medical College, Xinxiang, Henan 453003, P.R. China.
authors.forename: Jiaqin
authors.initials: J
authors.lastname: Wang
authors.affiliation: Department of Clinical Laboratory, Huai'an First People's Hospital, Nanjing Medical University, Huai'an, Jiangsu 223300, P.R. China.
authors.forename: Ning
authors.initials: N
authors.lastname: Lin
authors.affiliation: Department of Pediatrics, The Third Affiliated Hospital of Xinxiang Medical College, Xinxiang, Henan 453003, P.R. China.
authors.forename: Jiantao
authors.initials: J
authors.lastname: Chen
authors.affiliation: Department of Children Health Care, Nanjing Maternity and Child Health Care Hospital Affiliated to Nanjing Medical University, Nanjing, Jiangsu 210029, P.R. China.
authors.forename: Ling
authors.initials: L
authors.lastname: Chen
authors.affiliation: Department of Children Health Care, Nanjing Maternity and Child Health Care Hospital Affiliated to Nanjing Medical University, Nanjing, Jiangsu 210029, P.R. China.
authors.forename: Lei
authors.initials: L
authors.lastname: Yang
authors.affiliation: Department of Children Health Care, Nanjing Maternity and Child Health Care Hospital Affiliated to Nanjing Medical University, Nanjing, Jiangsu 210029, P.R. China.
authors.forename: Fangyan
authors.initials: F
authors.lastname: Huang
authors.affiliation: Department of Children Health Care, Nanjing Maternity and Child Health Care Hospital Affiliated to Nanjing Medical University, Nanjing, Jiangsu 210029, P.R. China.
authors.forename: Yahui
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: Department of Children Health Care, Nanjing Maternity and Child Health Care Hospital Affiliated to Nanjing Medical University, Nanjing, Jiangsu 210029, P.R. China.
authors.forename: Xirong
authors.initials: X
authors.lastname: Guo
authors.affiliation: Department of General Surgery, The First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu 210029, P.R. China.
authors.forename: Hui
authors.initials: H
authors.lastname: Liang
authors.affiliation: Department of Children Health Care, Nanjing Maternity and Child Health Care Hospital Affiliated to Nanjing Medical University, Nanjing, Jiangsu 210029, P.R. China.
authors.forename: Min
authors.initials: M
authors.lastname: Zhang
mesh_terms: D054392:Adipokines / Q000378:metabolism* / Q000494:pharmacology; D002454:Cell Differentiation / Q000187:drug effects; D002460:Cell Line; D016207:Cytokines / Q000378:metabolism* / Q000494:pharmacology; D005786:Gene Expression Regulation; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D035683:MicroRNAs / Q000235:genetics*; D009765:Obesity / Q000235:genetics* / Q000378:metabolism*; D011401:Promoter Regions, Genetic; D014158:Transcription, Genetic
chemical_list: D054392:Adipokines; D016207:Cytokines; C550570:MIRN148 microRNA, human; D035683:MicroRNAs