title: The effects of melatonin and colchicine on ischemia-reperfusion injury in experimental rat testicular torsion model.
abstract: OBJECTIVE
  The aim of the present study is to investigate the efficiency of colchicine and melatonın in an experimental rat testicular torsion model in the light of histological and biochemical data.
  
  
  METHODS
  A total of 34 Wistar albino male rats were randomly divided into 5 groups as: Group C (control, n=6), Group S (sham; underwent only left scrotal exploration, n=7), Group TD (torsion and detorsion; 6h of ischemia and 7days of reperfusion, n=7), Group TD/M (TD+Melatonin; 6h of ischemia and 7days of reperfusion and 7days of 17mg/kg intraperitoneal melatonin per day, n=7), group TD/Col (TD+Colchicine; 6h of ischemia and 7days of reperfusion and 7days of 1mg/kg oral colchicine per day, n=7). Histopathologic evaluation of seminiferous tubule deterioration was performed by Johnsen's scoring system. Total antioxidant status (TAS), total oxidant status (TOS), IL-6, TNF alpha levels were analyzed in each group.
  
  
  RESULTS
  The histopathologic scores, total antioxidant status (TAS), total oxidant status (TOS), IL-6, TNF alpha levels in groups C and TD/Col were significantly lower than groups TD and TD/M (P<.001).
  
  
  CONCLUSIONS
  Our study results revealed that colchicine reduced testicular ischemia-reperfusion injury in experimental rat testis torsion model. Although detorsion of testis is crucial for the preserving the testicular viability, antioxidant and anti-inflammatory treatment modalities like colchicine might help to reduce ischemia-reperfusion injury in detorsed testis.
authors.affiliation: Selcuk University Faculty of Medicine, Department of Pediatric Surgery. Electronic address: dr_sekmenli@hotmail.com.
authors.forename: Tamer
authors.initials: T
authors.lastname: Sekmenli
authors.affiliation: Selcuk University Faculty of Medicine, Department of Pediatric Surgery.
authors.forename: Metin
authors.initials: M
authors.lastname: Gunduz
authors.affiliation: Selcuk University Faculty of Medicine, Department of Medical Biochemistry.
authors.forename: Bahadır
authors.initials: B
authors.lastname: Öztürk
authors.affiliation: Selcuk University Faculty of Medicine, Department of Pathology.
authors.forename: Pınar
authors.initials: P
authors.lastname: Karabağlı
authors.affiliation: Selcuk University Faculty of Medicine, Department of Pediatric Surgery.
authors.forename: Ilhan
authors.initials: I
authors.lastname: Ciftci
authors.affiliation: Selcuk University Faculty of Medicine, Department of Medical Biochemistry.
authors.forename: Gülsüm
authors.initials: G
authors.lastname: Tekin
authors.affiliation: Selcuk University Faculty of Medicine, Department of Histology.
authors.forename: Mustafa
authors.initials: M
authors.lastname: Yılmaz
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000627:therapeutic use*; D000975:Antioxidants / Q000378:metabolism; D015415:Biomarkers / Q000378:metabolism; D003078:Colchicine / Q000627:therapeutic use*; D008297:Male; D008550:Melatonin / Q000627:therapeutic use*; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000209:etiology / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control*; D013086:Spermatic Cord Torsion / Q000473:pathology / Q000601:surgery*; D013737:Testis / Q000378:metabolism / Q000473:pathology; D016896:Treatment Outcome
chemical_list: D000893:Anti-Inflammatory Agents; D000975:Antioxidants; D015415:Biomarkers; D008550:Melatonin; D003078:Colchicine
keywords: Antioxidants; Colchicine; Melatonin; Rat testis; Reperfusion injury; Spermatic cord torsion