title: Efficient ex vivo delivery of chemically modified messenger RNA using lipofection and magnetofection.
abstract: Recently, chemically modified mRNA (cmRNA) therapeutics have been the subject of extensive application-oriented research in both academia and industry as a safer alternative for gene and recombinant protein therapies. However, the lack of an efficient delivery system hinders widespread application. Here we used ∼100-nm lipoplexes and magnetic lipoplexes that can protect cmRNA from RNases and efficiently deliver it into muscle and fat tissues as well as to the endothelium of the carotid artery. Establishing magnetofection for ex vivo cmRNA delivery for the first time, we suggest this method for potential enhanced and targeted delivery of cmRNA. This study introduces optimal cmRNA complexes with high ex vivo efficiency as good candidates for further in vivo cmRNA delivery.
authors.affiliation: Institute of Molecular Immunology-Experimental Oncology, Technische Universität München, Munich, Germany. Electronic address: badieeyanz82@yahoo.com.
authors.forename: Zohreh Sadat
authors.initials: ZS
authors.lastname: Badieyan
authors.affiliation: Ethris GmbH, Planegg, Germany.
authors.forename: Tamara
authors.initials: T
authors.lastname: Pasewald
authors.affiliation: Ethris GmbH, Planegg, Germany.
authors.forename: Olga
authors.initials: O
authors.lastname: Mykhaylyk
authors.affiliation: Ethris GmbH, Planegg, Germany.
authors.forename: Carsten
authors.initials: C
authors.lastname: Rudolph
authors.affiliation: Institute of Molecular Immunology-Experimental Oncology, Technische Universität München, Munich, Germany; Ethris GmbH, Planegg, Germany.
authors.forename: Christian
authors.initials: C
authors.lastname: Plank
mesh_terms: D000273:Adipose Tissue / Q000378:metabolism; D000818:Animals; D042783:Endothelial Cells / Q000378:metabolism; D008055:Lipids / Q000737:chemistry*; D008081:Liposomes / Q000737:chemistry; D008280:Magnetics / Q000379:methods*; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D051379:Mice; D009132:Muscles / Q000378:metabolism; D041681:NIH 3T3 Cells; D012333:RNA, Messenger / Q000008:administration & dosage* / Q000737:chemistry* / Q000235:genetics; D012756:Sheep; D013552:Swine; D014162:Transfection / Q000379:methods*
chemical_list: D008055:Lipids; D008081:Liposomes; D058185:Magnetite Nanoparticles; D012333:RNA, Messenger
keywords: Chemically modified mRNA (cmRNA); Lipoplex; Magnetofection; cmRNA delivery system