title: Bactericidal effect of plasma jet with helium flowing through 3% hydrogen peroxide againstEnterococcus faecalis.
abstract: The aim of the present study was to assess the antimicrobial activity of plasma jet with helium (He) flowing through 3% hydrogen peroxide in root canals infected withEnterococcus faecalis. A total of 42 single-rooted anterior teeth were prepared, sterilized, inoculated with anE. faecalissuspension and incubated for 7 days. Next, the teeth were randomly divided into six experimental groups (including groups treated by plasma jet with or without He for different time durations) and one control group treated without plasma. The number of surviving bacteria in each canal was determined by counting the colony forming units (CFU)/ml on nutrient agar plates. The results indicated that statistically significant reduction in CFU/ml (P<0.005) existed for all treatment groups relative to the control group. The greatest reductions in CFU/ml were observed for Group 3 (7.027 log unit reduction) and Group 2 (6.237 log unit reduction), which were treated by plasma jet sterilization with He flowing through 3% hydrogen peroxide for 4 min or for 2 min, respectively. In addition, the reduction in Group 3 was significantly greater compared with that in Group 2 or in the groups treated by plasma jet sterilization without He flowing through 3% hydrogen peroxide for 1, 2 or 4 min. In conclusion, plasma jet with or without He flowing through 3% hydrogen peroxide can effectively sterilized root canals infected withE. faecalisand should be considered as an alternative method for root canal disinfection in endodontic treatments.
authors.affiliation: Department of Stomatology, Baoan Maternal and Child Health Hospital, College of Medicine, Jinan University, Shenzhen, Guangdong 518133, P.R. China.
authors.forename: Xin-Cai
authors.initials: XC
authors.lastname: Zhou
authors.affiliation: Department of Stomatology, Baoan Maternal and Child Health Hospital, College of Medicine, Jinan University, Shenzhen, Guangdong 518133, P.R. China.
authors.forename: Yu-Lan
authors.initials: YL
authors.lastname: Li
authors.affiliation: Department of Stomatology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430030, P.R. China.
authors.forename: De-Xi
authors.initials: DX
authors.lastname: Liu
authors.affiliation: Department of Stomatology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430030, P.R. China.
authors.forename: Ying-Guang
authors.initials: YG
authors.lastname: Cao
authors.affiliation: College of Electrical and Electronic Engineering, Huazhong University of Science and Technology, Wuhan, Hubei 430074, P.R. China.
authors.forename: Xin-Pei
authors.initials: XP
authors.lastname: Lu
keywords: Enterococcus faecalis; antimicrobial efficacy; hydrogen peroxide; plasma jet