title: PpEst is a novel PBAT degrading polyesterase identified by proteomic screening of Pseudomonas pseudoalcaligenes.
abstract: A novel esterase, PpEst, that hydrolyses the co-aromatic-aliphatic polyester poly(1,4-butylene adipate-co-terephthalate) (PBAT) was identified by proteomic screening of the Pseudomonas pseudoalcaligenes secretome. PpEst was induced by the presence of PBAT in the growth media and had predicted arylesterase (EC 3.1.1.2) activity. PpEst showed polyesterase activity on both whole and milled PBAT film releasing terephthalic acid and 4-(4-hydroxybutoxycarbonyl)benzoic acid while end product inhibition by 4-(4-hydroxybutoxycarbonyl)benzoic acid was observed. Modelling of an aromatic polyester mimicking oligomer into the PpEst active site indicated that the binding pocket could be big enough to accommodate large polymers. This is the first report of a PBAT degrading enzyme being identified by proteomic screening and shows that this approach can contribute to the discovery of new polymer hydrolysing enzymes. Moreover, these results indicate that arylesterases could be an interesting enzyme class for identifications of polyesterases.
authors.affiliation: Research Unit for Functional Proteomics and Metabolic Pathways, Institute of Pathology, Medical University of Graz, Stiftingtalstrasse 24, 8010, Graz, Austria.|Austrian Centre of Industrial Biotechnology, Petersgasse 14, 8010, Graz, Austria.|Omics Center Graz, BioTechMed-Graz, Graz, Austria.
authors.forename: Paal W
authors.initials: PW
authors.lastname: Wallace
authors.affiliation: Austrian Centre of Industrial Biotechnology, Konrad Lorenz Strasse 20, 3430, Tulln, Austria.
authors.forename: Karolina
authors.initials: K
authors.lastname: Haernvall
authors.affiliation: Austrian Centre of Industrial Biotechnology, Konrad Lorenz Strasse 20, 3430, Tulln, Austria.|Institute of Environmental Biotechnology, University of Natural Resources and Life Sciences, Konrad Lorenz Strasse 20, 3430, Tulln, Vienna, Austria.
authors.forename: Doris
authors.initials: D
authors.lastname: Ribitsch
authors.affiliation: Austrian Centre of Industrial Biotechnology, Petersgasse 14, 8010, Graz, Austria.
authors.forename: Sabine
authors.initials: S
authors.lastname: Zitzenbacher
authors.affiliation: Research Unit for Functional Proteomics and Metabolic Pathways, Institute of Pathology, Medical University of Graz, Stiftingtalstrasse 24, 8010, Graz, Austria.|Omics Center Graz, BioTechMed-Graz, Graz, Austria.
authors.forename: Matthias
authors.initials: M
authors.lastname: Schittmayer
authors.affiliation: Austrian Centre of Industrial Biotechnology, Petersgasse 14, 8010, Graz, Austria.
authors.forename: Georg
authors.initials: G
authors.lastname: Steinkellner
authors.affiliation: Austrian Centre of Industrial Biotechnology, Petersgasse 14, 8010, Graz, Austria.|Institute of Molecular Biosciences, University of Graz, Humboldtstraße 50/III, 8010, Graz, Austria.
authors.forename: Karl
authors.initials: K
authors.lastname: Gruber
authors.affiliation: Austrian Centre of Industrial Biotechnology, Konrad Lorenz Strasse 20, 3430, Tulln, Austria.|Institute of Environmental Biotechnology, University of Natural Resources and Life Sciences, Konrad Lorenz Strasse 20, 3430, Tulln, Vienna, Austria.
authors.forename: Georg M
authors.initials: GM
authors.lastname: Guebitz
authors.affiliation: Research Unit for Functional Proteomics and Metabolic Pathways, Institute of Pathology, Medical University of Graz, Stiftingtalstrasse 24, 8010, Graz, Austria. ruth.birner-gruenberger@medunigraz.at.|Austrian Centre of Industrial Biotechnology, Petersgasse 14, 8010, Graz, Austria. ruth.birner-gruenberger@medunigraz.at.|Omics Center Graz, BioTechMed-Graz, Graz, Austria. ruth.birner-gruenberger@medunigraz.at.
authors.forename: Ruth
authors.initials: R
authors.lastname: Birner-Gruenberger
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D063566:Biodegradable Plastics / Q000737:chemistry / Q000378:metabolism*; D002265:Carboxylic Ester Hydrolases / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D020134:Catalytic Domain; D015870:Gene Expression; D008958:Models, Molecular; D010795:Phthalic Acids / Q000737:chemistry / Q000378:metabolism; D011091:Polyesters / Q000737:chemistry / Q000378:metabolism*; D011485:Protein Binding; D040901:Proteomics; D044205:Pseudomonas pseudoalcaligenes / Q000201:enzymology* / Q000235:genetics
chemical_list: D001426:Bacterial Proteins; D063566:Biodegradable Plastics; D010795:Phthalic Acids; D011091:Polyesters; C488797:poly(butylene adipate-co-butylene terephthalate); C011363:terephthalic acid; D002265:Carboxylic Ester Hydrolases; C019734:arylesterase
keywords: Arylesterase; Poly(1,4-butylene adipate-co-terephthalate) (PBAT); Polyesterase; Polymer degradation; Proteomics; Secretome