title: Microvascular lesions and changes in cell proliferation and death, and cytokine expression in the placentas of mice experimentally infected with Equid Herpesvirus 1.
abstract: This study describes the changes observed in the placentas of mice experimentally infected with an abortigenic strain of EHV-1 at mid-pregnancy and euthanized at days 3 and 4 post-infection. We analyzed microscopic vascular alterations, cell proliferation and death by immunohistochemistry, and the expression of IFN-γ, TNF-α and the IL-10 by qPCR and flow cytometry. Infected mice showed slight respiratory signs and ruffled fur during the first two days post-infection. Virus isolation and DNA detection were positive only in the lungs of the infected mice. Vascular congestion, increase in the labyrinth area, and a significant reduction in fetal capillary endothelium surface of infected placentas were found. Cell proliferation was significantly reduced in the infected placentas, whereas the apoptosis was significantly increased. IL10, TNF and IFN-γ showed different expression in the infected placentas and uteri. The effects of EHV-1 during pregnancy depend on different pathogenic mechanisms in which vascular alterations, and cell death and proliferation and local cytokine changes are compromised.
authors.affiliation: Department of Histology and Embryology, School of Veterinary Sciences, National University of La Plata, Argentina; National Research Council (CONICET), Argentina. Electronic address: carozanuzzi@gmail.com.
authors.forename: C N
authors.initials: CN
authors.lastname: Zanuzzi
authors.affiliation: Department of Virology, School of Veterinary Sciences, National University of La Plata, Argentina; Agency for the Promotion of Science and Technology (ANPCyT), Argentina.
authors.forename: M E
authors.initials: ME
authors.lastname: Bravi
authors.affiliation: Department of Virology, School of Veterinary Sciences, National University of La Plata, Argentina; Department of Histology and Embryology, School of Veterinary Sciences, National University of La Plata, Argentina; National Research Council (CONICET), Argentina.
authors.forename: M R
authors.initials: MR
authors.lastname: Scrochi
authors.affiliation: Image Analysis Laboratory, School of Veterinary Sciences, National University of La Plata, Argentina.
authors.forename: F
authors.initials: F
authors.lastname: Nishida
authors.affiliation: Department of Virology, School of Veterinary Sciences, National University of La Plata, Argentina; National Research Council (CONICET), Argentina.
authors.forename: N A
authors.initials: NA
authors.lastname: Fuentealba
authors.affiliation: Department of Histology and Embryology, School of Veterinary Sciences, National University of La Plata, Argentina.
authors.forename: M E
authors.initials: ME
authors.lastname: Diessler
authors.affiliation: Department of Virology, School of Veterinary Sciences, National University of La Plata, Argentina.
authors.forename: H G
authors.initials: HG
authors.lastname: Sguazza
authors.affiliation: Department of Immunopathology, Institute of Immunological and Physiopathological Studies (IIFP), Argentina; National Research Council (CONICET), Argentina.
authors.forename: C I
authors.initials: CI
authors.lastname: Muglia
authors.affiliation: National Research Council (CONICET), Argentina.
authors.forename: E J
authors.initials: EJ
authors.lastname: Gimeno
authors.affiliation: Image Analysis Laboratory, School of Veterinary Sciences, National University of La Plata, Argentina; National Research Council (CONICET), Argentina.
authors.forename: E L
authors.initials: EL
authors.lastname: Portiansky
authors.affiliation: Department of Histology and Embryology, School of Veterinary Sciences, National University of La Plata, Argentina; Image Analysis Laboratory, School of Veterinary Sciences, National University of La Plata, Argentina; National Research Council (CONICET), Argentina.
authors.forename: C G
authors.initials: CG
authors.lastname: Barbeito
authors.affiliation: Department of Virology, School of Veterinary Sciences, National University of La Plata, Argentina; Scientific Research Commission (CIC) of Province of Buenos Aires, Buenos Aires, Argentina.
authors.forename: C M
authors.initials: CM
authors.lastname: Galosi
mesh_terms: D000034:Abortion, Veterinary / Q000473:pathology* / Q000821:virology; D000818:Animals; D016923:Cell Death; D049109:Cell Proliferation; D016207:Cytokines / Q000235:genetics* / Q000378:metabolism; D005260:Female; D005434:Flow Cytometry; D006566:Herpesviridae Infections / Q000473:pathology / Q000662:veterinary* / Q000821:virology; D004861:Herpesvirus 1, Equid / Q000502:physiology; D007371:Interferon-gamma / Q000235:genetics / Q000378:metabolism; D016753:Interleukin-10 / Q000235:genetics / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D010920:Placenta / Q000473:pathology / Q000821:virology; D011247:Pregnancy; D060888:Real-Time Polymerase Chain Reaction; D013047:Specific Pathogen-Free Organisms; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics / Q000378:metabolism; D014599:Uterus / Q000473:pathology / Q000821:virology
chemical_list: D016207:Cytokines; C000611649:IFNG protein, mouse; C556062:IL10 protein, mouse; D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10; D007371:Interferon-gamma
keywords: Abortion; Apoptosis; Cytokines; Equid Herpesvirus 1; Mice; Proliferation