title: Stability behaviour of antiretroviral drugs and their combinations. 5: Characterization of novel degradation products of abacavir sulfate by mass and nuclear magnetic resonance spectrometry.
abstract: In the present study, degradation behaviour of abacavir sulfate was evaluated in solution and solid stress conditions. Solution state studies resulted in formation of eleven degradation products; of which two were also formed on solid stress. The same were separated by high performance liquid chromatography. They were characterized using liquid chromatography-high resolution mass spectrometry, liquid chromatography-multistage mass spectrometry and hydrogen/deuterium exchange mass spectrometry data. Additionally, seven degradation products were isolated and subjected to 1D and 2D nuclear magnetic resonance studies for their structural confirmation.
authors.affiliation: Department of Pharmaceutical Analysis, National Institute of Pharmaceutical Education and Research (NIPER), Sector 67, S.A.S. Nagar 160 062 Punjab, India.
authors.forename: Moolchand
authors.initials: M
authors.lastname: Kurmi
authors.affiliation: Department of Pharmaceutical Analysis, National Institute of Pharmaceutical Education and Research (NIPER), Sector 67, S.A.S. Nagar 160 062 Punjab, India.
authors.forename: Archana
authors.initials: A
authors.lastname: Sahu
authors.affiliation: Department of Pharmaceutical Analysis, National Institute of Pharmaceutical Education and Research (NIPER), Sector 67, S.A.S. Nagar 160 062 Punjab, India. Electronic address: ssingh@niper.ac.in.
authors.forename: Saranjit
authors.initials: S
authors.lastname: Singh
mesh_terms: D044966:Anti-Retroviral Agents / Q000032:analysis* / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D015224:Dideoxynucleosides / Q000032:analysis* / Q000737:chemistry; D004338:Drug Combinations; D004355:Drug Stability; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D013058:Mass Spectrometry / Q000379:methods; D000099227:Abacavir
chemical_list: D044966:Anti-Retroviral Agents; D015224:Dideoxynucleosides; D004338:Drug Combinations; D000099227:Abacavir
keywords: Abacavir sulfate; Degradation products; Mass spectrometry; NMR spectrometry; Stress testing