title: Marinobacter halotolerans sp. nov., a halophilic bacterium isolated from a saltern crystallizing pond.
abstract: A Gram-stain-negative, moderately halophilic, motile bacterium, designated strain CP12T, was isolated from a crystallizing pond of a saltern of the Yellow Sea in Korea. Cells of strain CP12T were non-spore-forming rods and produced whitish-yellow colonies. Growth was observed at 10-37 °C (optimum 37 °C), at pH 6.0-9.0 (optimum pH 7.0), and in the presence of 0.5-20 % (w/v) NaCl (optimum 3 %). Phylogenetic analysis based on the 16S rRNA gene sequence revealed that strain CP12T was closely related to Marinobacter flavimaris SW-145T (98.4 % 16S rRNA gene sequence similarity), Marinobacter algicola DG893T (98.2 %), Marinobacter adhaerens HP15T (98.2 %), Marinobacter salsuginis SD-14BT (97.9 %), Marinobacter salarius R9SW1T (97.6 %) and Marinobacter lipolyticus SM19T (97.1 %). DNA-DNA hybridization studies showed values lower than 18.6 % between strain CP12T and any of these species. The predominant respiratory isoprenoid quinone was ubiquinone-9 and the major cellular fatty acids of strain CP12T were C16 : 0, C12 : 0 3-OH, C12 : 0, Summed feature 3, C16 : 0 10-methyl and C18 : 1ω9c. On the basis of phenotypic properties, and phylogenetic and chemotaxonomic data, it is evident that strain CP12T represents a novel species of the genus Marinobacter, for which the name Marinobacter halotolerans sp. nov. is proposed. The type strain is CP12T (=KACC 18381T=NBRC 110910T).
authors.affiliation: Department of Microbial & Nano Materials, Mokwon University, Daejeon 302-729, Republic of Korea.
authors.forename: Ju-Ok
authors.initials: JO
authors.lastname: Kim
authors.affiliation: Institute of Microbial Ecology & Resources, Mokwon University, Daejeon 302-729, Republic of Korea.
authors.forename: Hyo-Jin
authors.initials: HJ
authors.lastname: Lee
authors.affiliation: Department of Microbial & Nano Materials, Mokwon University, Daejeon 302-729, Republic of Korea.
authors.forename: Song-Ih
authors.initials: SI
authors.lastname: Han
authors.affiliation: Institute of Microbial Ecology & Resources, Mokwon University, Daejeon 302-729, Republic of Korea.|Department of Microbial & Nano Materials, Mokwon University, Daejeon 302-729, Republic of Korea.
authors.forename: Kyung-Sook
authors.initials: KS
authors.lastname: Whang
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D053520:Marinobacter / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D061005:Ponds / Q000382:microbiology*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D056910:Republic of Korea; D054712:Salinity; D017422:Sequence Analysis, DNA; D014451:Ubiquinone / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D014451:Ubiquinone; C030571:ubiquinone 9