title: Dietary Mung Bean Protein Reduces Hepatic Steatosis, Fibrosis, and Inflammation in Male Mice with Diet-Induced, Nonalcoholic Fatty Liver Disease.
abstract: As the prevalence of nonalcoholic fatty liver disease (NAFLD), including steatosis and nonalcoholic steatohepatitis, is increasing, novel dietary approaches are required for the prevention and treatment of NAFLD.
  
  
  
  We evaluated the potential of mung bean protein isolate (MuPI) to prevent NAFLD progression.
  
  
  
  In Expts. 1 and 2, the hepatic triglyceride (TG) concentration was compared between 8-wk-old male mice fed a high-fat diet (61% of energy from fat) containing casein, MuPI, and soy protein isolate and an MuPI-constituent amino acid mixture as a source of amino acids (18% of energy) for 4 wk. In Expt. 3, hepatic fatty acid synthase (Fasn) expression was evaluated in 8-wk-old male Fasn-promoter-reporter mice fed a casein- or MuPI-containing high-fat diet for 20 wk. In Expt. 4, hepatic fibrosis was examined in 8-wk-old male mice fed an atherogenic diet (61% of energy from fat, containing 1.3 g cholesterol/100 g diet) containing casein or MuPI (18% of energy) as a protein source for 20 wk.
  
  
  
  In the high fat-diet mice, the hepatic TG concentration in the MuPI group decreased by 66% and 47% in Expt. 1 compared with the casein group (P < 0.001) and the soy protein isolate group (P = 0.001), respectively, and decreased by 56% in Expt. 2 compared with the casein group (P = 0.011). However, there was no difference between the MuPI-constituent amino acid mixture and casein groups in Expt. 2. In Expt. 3, Fasn-promoter-reporter activity and hepatic TG concentration were lower in the MuPI group than in those fed casein (P < 0.05). In Expt. 4, in mice fed an atherogenic diet, hepatic fibrosis was not induced in the MuPI group, whereas it developed overtly in the casein group.
  
  
  
  MuPI potently reduced hepatic lipid accumulation in mice and may be a potential foodstuff to prevent NAFLD onset and progression.
authors.affiliation: Metabolism and Nutrition Research Unit, Institute for Frontier Science Initiative.|Department of Physiology and Metabolism, Brain/Liver Interface Medicine Research Center, and.
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Watanabe
authors.affiliation: Metabolism and Nutrition Research Unit, Institute for Frontier Science Initiative.|Department of Physiology and Metabolism, Brain/Liver Interface Medicine Research Center, and.
authors.forename: Yuka
authors.initials: Y
authors.lastname: Inaba
authors.affiliation: Department of Physiology and Metabolism, Brain/Liver Interface Medicine Research Center, and.
authors.forename: Kumi
authors.initials: K
authors.lastname: Kimura
authors.affiliation: Division of Diabetes and Endocrinology, Kobe University Graduate School of Medicine, Kobe, Hyogo, Japan.
authors.forename: Shun-Ichiro
authors.initials: SI
authors.lastname: Asahara
authors.affiliation: Division of Diabetes and Endocrinology, Kobe University Graduate School of Medicine, Kobe, Hyogo, Japan.|Division of Medical Chemistry, Department of Biophysics, Kobe University Graduate School of Health Sciences, Kobe, Hyogo, Japan.
authors.forename: Yoshiaki
authors.initials: Y
authors.lastname: Kido
authors.affiliation: Department of Molecular Metabolic Regulation, Diabetes Research Center, Research Institute, National Center for Global Health and Medicine, Tokyo, Japan; and.
authors.forename: Michihiro
authors.initials: M
authors.lastname: Matsumoto
authors.affiliation: Health and Nutrition Research Department, Research and Development Division for Future Creation, Fuji Oil Co., Ltd., Tsukuba-mirai, Ibaraki, Japan.
authors.forename: Takayasu
authors.initials: T
authors.lastname: Motoyama
authors.affiliation: Health and Nutrition Research Department, Research and Development Division for Future Creation, Fuji Oil Co., Ltd., Tsukuba-mirai, Ibaraki, Japan.
authors.forename: Nobuhiko
authors.initials: N
authors.lastname: Tachibana
authors.affiliation: Department of Disease Control and Homeostasis, Graduate School of Medical Sciences, Kanazawa University, Kanazawa, Ishikawa, Japan.
authors.forename: Shuichi
authors.initials: S
authors.lastname: Kaneko
authors.affiliation: Health and Nutrition Research Department, Research and Development Division for Future Creation, Fuji Oil Co., Ltd., Tsukuba-mirai, Ibaraki, Japan.
authors.forename: Mitsutaka
authors.initials: M
authors.lastname: Kohno
authors.affiliation: Metabolism and Nutrition Research Unit, Institute for Frontier Science Initiative, inoue-h@staff.kanazawa-u.ac.jp.|Department of Physiology and Metabolism, Brain/Liver Interface Medicine Research Center, and.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Inoue
mesh_terms: D000818:Animals; D004041:Dietary Fats / Q000633:toxicity; D004044:Dietary Proteins / Q000008:administration & dosage* / Q000032:analysis; D054890:Fatty Acid Synthase, Type I / Q000378:metabolism; D005234:Fatty Liver / Q000139:chemically induced / Q000517:prevention & control*; D005786:Gene Expression Regulation; D007249:Inflammation / Q000378:metabolism / Q000517:prevention & control*; D008103:Liver Cirrhosis / Q000378:metabolism / Q000517:prevention & control*; D008156:Luciferases / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D000070658:Vigna / Q000737:chemistry*
chemical_list: D004041:Dietary Fats; D004044:Dietary Proteins; D008156:Luciferases; D054890:Fatty Acid Synthase, Type I
keywords: de novo lipogenesis; hepatic fibrosis; hepatic steatosis; mung bean protein isolate; nonalcoholic fatty liver disease; soy protein isolate