title: Improvement Production of Hyaluronic Acid by Streptococcus zooepidemicus in Sugarcane Molasses.
abstract: Microbial hyaluronic acid (HA) production has been preferred rather than extraction from animal tissue for medical and cosmetic applications. In this context, to obtain an economically competitive HA production by Streptococcus zooepidemicus, culture conditions were studied to improve the polymer production in sugarcane molasses. The highest HA production by S. zooepidemicus ATCC 39920 achieved was 2.825 g. L-1in a 4.5 L bioreactor with controlled pH (8.0) and medium containing molasses (85.35 g.L-1total sugar) pretreated with activated charcoal and yeast extract (50 g.L-1). The HA produced exhibited a high molecular weight of 1.35 × 103kDa and the DPPH radical scavenging activity of the polymer at 1 g.L-1was 41 %. The FTIR and UV-Vis spectra showed no substantial differences in the spectral pattern between produced and standard HA. This study is a promising strategy for sugarcane molasses application by producing high value-added products such as hyaluronic acid.
authors.affiliation: Departamento de Bioquímica e Biotecnologia, Universidade Estadual de Londrina - UEL, Rodovia Celso Garcia Cid - Pr 445 Km 380, Campus Universitário, Caixa Postal 10.011, Londrina, PR, CEP 86.057-970, Brazil.
authors.forename: Nicole Caldas
authors.initials: NC
authors.lastname: Pan
authors.affiliation: Departamento de Bioquímica e Biotecnologia, Universidade Estadual de Londrina - UEL, Rodovia Celso Garcia Cid - Pr 445 Km 380, Campus Universitário, Caixa Postal 10.011, Londrina, PR, CEP 86.057-970, Brazil.
authors.forename: Hanny Cristina Braga
authors.initials: HCB
authors.lastname: Pereira
authors.affiliation: Departamento de Química e Bioquímica, Faculdade de Ciências e Tecnologia, Universidade Estadual Paulista -UNESP, Rua Roberto Simonsen, n°. 305, Presidente Prudente, São Paulo, CEP 19060-900, Brazil.
authors.forename: Maria de Lourdes Corradi
authors.initials: MLC
authors.lastname: da Silva
authors.affiliation: Departamento de Química e Bioquímica, Faculdade de Ciências e Tecnologia, Universidade Estadual Paulista -UNESP, Rua Roberto Simonsen, n°. 305, Presidente Prudente, São Paulo, CEP 19060-900, Brazil.
authors.forename: Ana Flora Dalberto
authors.initials: AFD
authors.lastname: Vasconcelos
authors.affiliation: Departamento de Bioquímica e Biotecnologia, Universidade Estadual de Londrina - UEL, Rodovia Celso Garcia Cid - Pr 445 Km 380, Campus Universitário, Caixa Postal 10.011, Londrina, PR, CEP 86.057-970, Brazil. macelligoi@uel.br.
authors.forename: Maria Antonia Pedrine Colabone
authors.initials: MAPC
authors.lastname: Celligoi
mesh_terms: D019149:Bioreactors; D001713:Biphenyl Compounds / Q000037:antagonists & inhibitors; D003470:Culture Media / Q000737:chemistry; D005163:Factor Analysis, Statistical; D005285:Fermentation; D016166:Free Radical Scavengers / Q000737:chemistry / Q000378:metabolism*; D006820:Hyaluronic Acid / Q000096:biosynthesis* / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D008965:Molasses; D010851:Picrates / Q000037:antagonists & inhibitors; D031786:Saccharum / Q000737:chemistry*; D018502:Streptococcus equi / Q000378:metabolism*
chemical_list: D001713:Biphenyl Compounds; D003470:Culture Media; D016166:Free Radical Scavengers; D010851:Picrates; D006820:Hyaluronic Acid; C004931:1,1-diphenyl-2-picrylhydrazyl
keywords: Bioreactor; Fermentation; Hyaluronic acid; Streptococcus; Sugarcane molasses