title: Electrospun cellulose acetate membrane for size separating and antibacterial screening of crude polysaccharides.
abstract: This study aims to produce electrospun cellulose acetate (CA) membrane as the alternative supporting medium for a separation of crude polysaccharides by electrophoresis and a screening of their antibacterial activity. Among the tested conditions of fabrication, electrospun CA membrane at 57% porosity showed the best separation of each polysaccharide from the standard mixture and the crude extract ofAloe veravia electrophoresis. As compared with the commercial CA membrane, the produced electrospun CA membrane demonstrated more separated spots of polysaccharides. The antibacterial activity of the electrophoretic polysaccharide was also determined againstEscherichia coliandStaphylococcus aureusas the inhibition zone after the bacterial culture agar was overlaid on the membrane and incubated for 24 h. The results of this study suggested the potential application of electrospun CA membrane combining with electrophoresis as a simple method for separating crude polysaccharides and screening for their antibacterial activity.
authors.affiliation: Department of Biochemistry, Faculty of Science, Khon Kaen University, Khon Kaen, Thailand.
authors.forename: Jiraporn
authors.initials: J
authors.lastname: Chumpol
authors.affiliation: School of Biology, Institute of Science, Suranaree University of Technology, Nakhon Ratchasima, Thailand. ssinee@sut.ac.th.
authors.forename: Sineenat
authors.initials: S
authors.lastname: Siri
mesh_terms: D000900:Anti-Bacterial Agents / Q000032:analysis* / Q000494:pharmacology; D002482:Cellulose / Q000031:analogs & derivatives*; D004926:Escherichia coli / Q000187:drug effects; D011134:Polysaccharides / Q000032:analysis* / Q000494:pharmacology; D013211:Staphylococcus aureus / Q000187:drug effects
chemical_list: D000900:Anti-Bacterial Agents; D011134:Polysaccharides; C005062:acetylcellulose; D002482:Cellulose