title: Bacillus piscis sp. nov., a novel bacterium isolated from the muscle of the antarctic fish Dissostichus mawsoni.
abstract: In this paper, a new bacterial strain designated as 16MFT21Tis isolated from the muscle of a fish caught in the Antarctic Ocean. Strain 16MFT21Tis a Gram-staining-positive, catalase-oxidase-positive, rod-shaped facultative-aerobic bacterium. The phylogenetic analysis that is based on the 16S-rRNA gene sequence of strain 16MFT21Trevealed that it belongs to the genus Bacillus in the family Bacillaceae in the class Bacilli. The highest degrees of the sequence similarity of the strain 16MFT21Tis with Bacillus licheniformis ATCC 14580T(96.6%) and Bacillus sonorensis NBRC 101234T(96.6%). The isolate formed a pale-yellow pigment, and it grew in the presence of 0% to 10% (w/v) NaCl (optimum at 2% NaCl), a pH of 6.0 to 10.0 (optimum pH from 7.0 to 8.0), and from 4°C to 30°C (optimum at 30°C). The major polar lipids consist of diphosphatidylglycerol (DPG) and phosphatidylglycerol (PG). The predominant fatty acids are iso-C15:0, anteiso-C15:0, iso-C17:0, and anteiso-C17:0. The main respiratory quinone is menaquinone-7 (MK-7), and based on the use of the meso-diaminopimelic acid as the diagnostic diamino acid, the peptidoglycan cell-wall type is A1γ. Based on the phylogenetic, phenotypic, and chemotaxonomic data, strain 16MFT21T(=KCTC 18866T=JCM 31664T) for which the name Bacillus piscis sp. nov. is proposed should be classified as a new species.
authors.affiliation: Distant-water Fisheries Resources Division, National Institute of Fisheries Science, Busan, 46083, Republic of Korea.
authors.forename: Jae-Bong
authors.initials: JB
authors.lastname: Lee
authors.affiliation: Department of Bio and Environmental Technology, College of Natural Science, Seoul Women's University, Seoul, 01797, Republic of Korea.
authors.forename: Seon Hwa
authors.initials: SH
authors.lastname: Jeon
authors.affiliation: Distant-water Fisheries Resources Division, National Institute of Fisheries Science, Busan, 46083, Republic of Korea.
authors.forename: Seok-Gwan
authors.initials: SG
authors.lastname: Choi
authors.affiliation: College of Agricultural and Life Sciences, Kyungpook National University, Daegu, 41566, Republic of Korea.|Institute of Plant Medicine, Kyungpook National University, Daegu, 41566, Republic of Korea.
authors.forename: Hee-Young
authors.initials: HY
authors.lastname: Jung
authors.affiliation: Department of Bio and Environmental Technology, College of Natural Science, Seoul Women's University, Seoul, 01797, Republic of Korea.
authors.forename: Myung Kyum
authors.initials: MK
authors.lastname: Kim
authors.affiliation: Department of Bio and Environmental Technology, College of Natural Science, Seoul Women's University, Seoul, 01797, Republic of Korea. drsrini@swu.ac.kr.
authors.forename: Sathiyaraj
authors.initials: S
authors.lastname: Srinivasan
mesh_terms: D000818:Animals; D000864:Antarctic Regions; D001407:Bacillus / Q000737:chemistry / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal; D005227:Fatty Acids / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D009132:Muscles / Q000382:microbiology*; D009792:Oceans and Seas; D010457:Peptidoglycan / Q000737:chemistry; D010473:Perciformes / Q000033:anatomy & histology / Q000382:microbiology*; D010715:Phosphatidylglycerols / Q000737:chemistry; D010743:Phospholipids / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S; D017422:Sequence Analysis, DNA
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D010457:Peptidoglycan; D010715:Phosphatidylglycerols; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S
keywords: Antarctic Ocean; Antarctic toothfish; Bacillus; Dissostichus mawsoni; taxonomy