title: An IC-MS/MS Method for the Determination of 1-Hydroxyethylidene-1,1-diphosphonic Acid on Uncooked Foods Treated with Peracetic Acid-Based Sanitizers.
abstract: A rapid, sensitive, and specific analytical method for the determination of 1-hydroxyethylidene-1,1-diphosphonic acid (HEDP) on uncooked foods after treatment with a peracetic acid-based sanitizer (PAS) was developed. The method involves simple sample preparation steps and analysis using ion chromatography (IC) coupled with tandem mass spectrometry (MS/MS). The quantification limits of HEDP on uncooked foods are 0.007 mg/kg for vegetables and fruits and 0.2 mg/kg for meats. The recovery and relative standard deviation (RSD) of HEDP analyses of uncooked foods ranged from 73.9 to 103.8% and 1.9 to 12.6%, respectively. The method's accuracy and precision were evaluated by inter-day recovery tests. The recovery for all samples ranged from 93.6 to 101.2%, and the within-laboratory repeatability and reproducibility were evaluated based on RSD values, which were less than 6.9 and 11.5%, respectively. Analyses of PAS-treated fruits and vegetables using the developed method indicated levels of HEDP ranging from 0.008 to 0.351 mg/kg. Therefore, the results of the present study suggest that the proposed method is an accurate, precise, and reliable way to determine residual HEDP levels on PAS-treated uncooked foods.
authors.affiliation: National Institute of Health Sciences.
authors.forename: Ippei
authors.initials: I
authors.lastname: Suzuki
authors.forename: Hiroki
authors.initials: H
authors.lastname: Kubota
authors.forename: Takashi
authors.initials: T
authors.lastname: Ohtsuki
authors.forename: Chiye
authors.initials: C
authors.lastname: Tatebe
authors.forename: Atsuko
authors.initials: A
authors.lastname: Tada
authors.forename: Takeo
authors.initials: T
authors.lastname: Yano
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Akiyama
authors.forename: Kyoko
authors.initials: K
authors.lastname: Sato
mesh_terms: D000891:Anti-Infective Agents, Local / Q000032:analysis / Q000737:chemistry*; D012968:Etidronic Acid / Q000032:analysis* / Q000737:chemistry; D010463:Peracetic Acid / Q000737:chemistry / Q000494:pharmacology*; D066256:Raw Foods; D013057:Spectrum Analysis; D053719:Tandem Mass Spectrometry
chemical_list: D000891:Anti-Infective Agents, Local; D010463:Peracetic Acid; D012968:Etidronic Acid