title: MiR-4295 promotes cell growth in bladder cancer by targeting BTG1.
abstract: microRNAs (miRNAs) have been demonstrated to contribute to tumor progression and metastasis, and have been proposed to be key regulators of diverse biological processes. In this study, we report that miR-4295 is deregulated in bladder cancer tissues and cell lines. To characterize the role of miR-4295 in bladder cancer cells, we performed functional assays. The overexpression of miR-4295 significantly promoted bladder cancer cell proliferation, colony formation, and migration. Moreover, its downregulation induced cell cycle arrest and apoptosis of bladder cancer cells. Furthermore, a luciferase reporter assay and rescue experiment indicated that miR-4295 directly targets BTG1 by binding its 3'UTR. In conclusion, these results demonstrate that miR-4295 acts as an oncogene and may be a potential biomarker for bladder cancer diagnosis and treatment.
authors.affiliation: Department of Urology, China-Japan Union Hospital of Jilin University Changchun, Jilin 130033, China.
authors.forename: Yong-Hao
authors.initials: YH
authors.lastname: Nan
authors.affiliation: Clinical Medicine School, Ningxia Medical University Yinchuan, Ningxia 75004, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Wang
authors.affiliation: Department of Urology, China-Japan Union Hospital of Jilin University Changchun, Jilin 130033, China.
authors.forename: Yao
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Andrology, The First Hospital of Jilin University Changchun, Jilin 130021, China.
authors.forename: Peng-Hao
authors.initials: PH
authors.lastname: Sun
authors.affiliation: Department of Urology, China-Japan Union Hospital of Jilin University Changchun, Jilin 130033, China.
authors.forename: Yu-Ping
authors.initials: YP
authors.lastname: Han
authors.affiliation: Department of Urology, China-Japan Union Hospital of Jilin University Changchun, Jilin 130033, China.
authors.forename: Li
authors.initials: L
authors.lastname: Fan
authors.affiliation: Department of Urology, China-Japan Union Hospital of Jilin University Changchun, Jilin 130033, China.
authors.forename: Kai-Chen
authors.initials: KC
authors.lastname: Wang
authors.affiliation: Department of Oncology, Yancheng Hospital Affiliated to Medical College of Southeast University & The Third People's Hospital of Yancheng Yancheng, Jiangsu 224001, China.
authors.forename: Fu-Jun
authors.initials: FJ
authors.lastname: Shen
authors.affiliation: Department of Urology, China-Japan Union Hospital of Jilin University Changchun, Jilin 130033, China.
authors.forename: Wei-Hua
authors.initials: WH
authors.lastname: Wang
keywords: BTG1; MiR-4295; bladder cancer; cell growth