title: Improvement of functionality after chitosan-modified zein biocomposites.
abstract: A series of chitosan-modified zein composite films were fabricated from zein and chitosan by a process involving blending, solution casting and evaporation. Effects of chitosan content on the structure and physical properties of the composite films were investigated by Fourier transform infrared spectroscopy, differential scanning calorimetry, scanning electron microscopy, tensile testing, water absorption measurement and water contact angle measurement. The results showed that the zein/chitosan composite films were fabricated successfully due to the formation of hydrogen bonds between zein and chitosan, and the thermal stability, water absorption, hydrophilicity, tensile strength, flexibility of the composite films increased with an increase in chitosan content from 0 to 50%. The cytotoxicity and cytocompatibility of the composite films were evaluated by 3-[45-dimethyl-2-thiazoly1]-25-diphenyl-2H-tetrazolium bromide (MTT) assay and in vitro cell culture, which showed that the films have non- or low-cytotoxicity, and chitosan promoted the growth, adhesion and proliferation of the cells. These results indicated that chitosan-modified zein composite films might have potentials applications as biomaterials.
authors.affiliation: a UMR 7365 CNRS - Université de Lorraine, Ingénierie Moléculaire et Physiopathologie Articulaire (IMoPA) , Biopôle , Vandoeuvre-lès-Nancy , France.|b Fédération de Recherche 3209, Bioingénierie Moléculaire Cellulaire et Thérapeutique , Vandoeuvre-lès-Nancy , France.
authors.forename: Hao
authors.initials: H
authors.lastname: Yu
authors.affiliation: c Department of Biochemistry and Molecular Biology, School of Life Science , Hubei University , Wuhan , China.
authors.forename: Wei
authors.initials: W
authors.lastname: Li
authors.affiliation: a UMR 7365 CNRS - Université de Lorraine, Ingénierie Moléculaire et Physiopathologie Articulaire (IMoPA) , Biopôle , Vandoeuvre-lès-Nancy , France.|b Fédération de Recherche 3209, Bioingénierie Moléculaire Cellulaire et Thérapeutique , Vandoeuvre-lès-Nancy , France.
authors.forename: Xing
authors.initials: X
authors.lastname: Liu
authors.affiliation: d Department of Biomedical Engineering, School of Basic Medical Sciences , Wuhan University , Wuhan , China.
authors.forename: Chen
authors.initials: C
authors.lastname: Li
authors.affiliation: c Department of Biochemistry and Molecular Biology, School of Life Science , Hubei University , Wuhan , China.
authors.forename: Hong
authors.initials: H
authors.lastname: Ni
authors.affiliation: a UMR 7365 CNRS - Université de Lorraine, Ingénierie Moléculaire et Physiopathologie Articulaire (IMoPA) , Biopôle , Vandoeuvre-lès-Nancy , France.
authors.forename: Xiong
authors.initials: X
authors.lastname: Wang
authors.affiliation: a UMR 7365 CNRS - Université de Lorraine, Ingénierie Moléculaire et Physiopathologie Articulaire (IMoPA) , Biopôle , Vandoeuvre-lès-Nancy , France.|b Fédération de Recherche 3209, Bioingénierie Moléculaire Cellulaire et Thérapeutique , Vandoeuvre-lès-Nancy , France.
authors.forename: Céline
authors.initials: C
authors.lastname: Huselstein
authors.affiliation: d Department of Biomedical Engineering, School of Basic Medical Sciences , Wuhan University , Wuhan , China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Chen
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry* / Q000494:pharmacology*; D002460:Cell Line; D048271:Chitosan / Q000737:chemistry*; D057927:Hydrophobic and Hydrophilic Interactions; D008422:Materials Testing; D051379:Mice; D013499:Surface Properties; D013718:Tensile Strength; D014867:Water / Q000737:chemistry; D015028:Zein / Q000737:chemistry*
chemical_list: D001672:Biocompatible Materials; D014867:Water; D015028:Zein; D048271:Chitosan
keywords: Zein; chitosan; cytocompatibility; hydrophilicity; mechanical properties