title: A peptidoglycan recognition protein acts in whitefly (Bemisia tabaci) immunity and involves in Begomovirus acquisition.
abstract: Peptidoglycan recognition proteins (PGRPs) are multifunctional pattern recognition proteins. Here, we report that a PGRP gene, BtPGRP, encodes a PGRP from the whitefly Bemisia tabaci (MEAM1) that binds and kills bacteria in vitro. We analyzed BtPGRP transcriptional profiling, and the distribution of the cognate protein within the midgut. Fungal infection and wasp parasitization induced expression of BtPGRP. Silencing BtPGRP with artificial media amended with dsRNA led to reduced expression of a gene encoding an antimicrobial peptide, B. tabaci c-type lysozyme. Begomovirus infection also led to increased expression of BtPGRP. We propose that BtPGRP has a potential Tomato yellow leaf curl virus (TYLCV) binding site because we detected in vitro interaction between BtPGRP and TYLCV by immunocapture PCR, and recorded the co-localization of TYLCV and BtPGRP in midguts. This work addresses a visible gap in understanding whitefly immunity and provides insight into how the whitefly immunity acts in complex mechanisms of Begomovirus transmission among plants.
authors.affiliation: Ministry of Agriculture Key Lab of Agricultural Entomology, Institute of Insect Sciences, Zhejiang University, 866 Yuhangtang Road, Hangzhou 310058, China.
authors.forename: Zhi-Zhi
authors.initials: ZZ
authors.lastname: Wang
authors.affiliation: Ministry of Agriculture Key Lab of Agricultural Entomology, Institute of Insect Sciences, Zhejiang University, 866 Yuhangtang Road, Hangzhou 310058, China.
authors.forename: Min
authors.initials: M
authors.lastname: Shi
authors.affiliation: Ministry of Agriculture Key Lab of Agricultural Entomology, Institute of Insect Sciences, Zhejiang University, 866 Yuhangtang Road, Hangzhou 310058, China.
authors.forename: Yi-Cun
authors.initials: YC
authors.lastname: Huang
authors.affiliation: Ministry of Agriculture Key Lab of Agricultural Entomology, Institute of Insect Sciences, Zhejiang University, 866 Yuhangtang Road, Hangzhou 310058, China.
authors.forename: Xiao-Wei
authors.initials: XW
authors.lastname: Wang
authors.affiliation: Biological Control of Insects Research Laboratory, Agricultural Research Service, U.S., Department of Agriculture, 1503 S. Providence Road, Columbia MO 65203, USA.
authors.forename: David
authors.initials: D
authors.lastname: Stanley
authors.affiliation: Ministry of Agriculture Key Lab of Agricultural Entomology, Institute of Insect Sciences, Zhejiang University, 866 Yuhangtang Road, Hangzhou 310058, China.
authors.forename: Xue-Xin
authors.initials: XX
authors.lastname: Chen
mesh_terms: D000818:Animals; D001483:Base Sequence; D053839:Begomovirus / Q000502:physiology*; D036022:Capsid Proteins / Q000378:metabolism; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism* / Q000494:pharmacology; D003001:Cloning, Molecular; D004064:Digestive System / Q000821:virology; D005786:Gene Expression Regulation / Q000187:drug effects; D006430:Hemiptera / Q000276:immunology* / Q000821:virology*; D007109:Immunity; D018551:Solanum lycopersicum / Q000821:virology; D008826:Microbial Sensitivity Tests; D010802:Phylogeny; D012330:RNA, Double-Stranded / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011994:Recombinant Proteins / Q000378:metabolism; D017422:Sequence Analysis, DNA
chemical_list: D036022:Capsid Proteins; D002352:Carrier Proteins; D012330:RNA, Double-Stranded; D012333:RNA, Messenger; D011994:Recombinant Proteins; C118897:peptidoglycan recognition protein