title: SPOCK1 is up-regulated and promotes tumor growth via the PI3K/AKT signaling pathway in colorectal cancer.
abstract: SPOCK1 encodes a Ca2+-binding matricellular glycoprotein which plays an oncogenic role in cancer cells. However, the role of SPOCK1 in the pathogenesis of colorectal cancer (CRC) has not been determined. Here, SPOCK1 was found higher expressed in CRC tissues than that of adjacent normal tissues. Furthermore, up-regulated expression of SPOCK1 in CRC patients was associated with tumor size and TNM stage. In addition, we observed that the depletion of SPOCK1 inhibited proliferation in vitro and tumorigenicity in vivo and promoted apoptosis in cell culture. Our data suggest that inactivation of PI3K/Akt signaling pathway was involved in down-regulation of SPOCK1-mediated suppression of tumor cell proliferation. These results suggest that SPOCK1 expression is correlated with malignant features of CRC and may serve as potential therapeutic and preventive strategies for CRC.
authors.affiliation: Department of General Surgery, Affiliated Hospital of Nantong University, Nantong, 226001, Jiangsu, China.
authors.forename: Jiaxuan
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Department of General Surgery, Affiliated Hospital of Nantong University, Nantong, 226001, Jiangsu, China.
authors.forename: Xiaofei
authors.initials: X
authors.lastname: Zhi
authors.affiliation: Medical College, Nantong University, Nantong, 226001, Jiangsu, China.
authors.forename: Shiyu
authors.initials: S
authors.lastname: Shi
authors.affiliation: Department of General Surgery, Affiliated Hospital of Nantong University, Nantong, 226001, Jiangsu, China.
authors.forename: Ran
authors.initials: R
authors.lastname: Tao
authors.affiliation: Department of General Surgery, Affiliated Hospital of Nantong University, Nantong, 226001, Jiangsu, China.
authors.forename: Peisheng
authors.initials: P
authors.lastname: Chen
authors.affiliation: Department of General Surgery, Affiliated Hospital of Nantong University, Nantong, 226001, Jiangsu, China.
authors.forename: Shiyu
authors.initials: S
authors.lastname: Sun
authors.affiliation: Department of General Surgery, Affiliated Hospital of Nantong University, Nantong, 226001, Jiangsu, China.
authors.forename: Longjun
authors.initials: L
authors.lastname: Bian
authors.affiliation: Medical College, Nantong University, Nantong, 226001, Jiangsu, China.
authors.forename: Zhangyan
authors.initials: Z
authors.lastname: Xu
authors.affiliation: Department of General Surgery, Affiliated Hospital of Nantong University, Nantong, 226001, Jiangsu, China. Electronic address: mall9052@163.com.
authors.forename: Lilin
authors.initials: L
authors.lastname: Ma
mesh_terms: D000818:Animals; D017209:Apoptosis; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D003106:Colon / Q000378:metabolism / Q000473:pathology*; D015179:Colorectal Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D008875:Middle Aged; D019869:Phosphatidylinositol 3-Kinases / Q000378:metabolism*; D011509:Proteoglycans / Q000032:analysis / Q000235:genetics* / Q000378:metabolism; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism*; D012007:Rectum / Q000378:metabolism / Q000473:pathology*; D015398:Signal Transduction; D015854:Up-Regulation
chemical_list: D011509:Proteoglycans; C504369:SPOCK1 protein, human; D019869:Phosphatidylinositol 3-Kinases; D051057:Proto-Oncogene Proteins c-akt
keywords: Cell apoptosis; Cell proliferation; Colorectal cancer; PI3K/AKT pathway; SPOCK1