title: Sodium butyrate attenuates soybean oil-based lipid emulsion-induced increase in intestinal permeability of lipopolysaccharide by modulation of P-glycoprotein in Caco-2 cells.
abstract: Down-regulation of intestinal P-glycoprotein (P-gp) by soybean oil-based lipid emulsion (SOLE) may cause elevated intestinal permeability of lipopolysaccharide (LPS) in patients with total parenteral nutrition, but the appropriate preventative treatment is currently limited. Recently, sodium butyrate (NaBut) has been demonstrated to regulate the expression of P-gp. Therefore, this study aimed to address whether treatment with NaBut could attenuate SOLE-induced increase in intestinal permeability of LPS by modulation of P-gp in vitro. Caco-2 cells were exposed to SOLE with or without NaBut. SOLE-induced down-regulation of P-gp was significantly attenuated by co-incubation with NaBut. Nuclear recruitment of FOXO 3a in response to NaBut was involved in P-gp regulation. Transport studies revealed that SOLE-induced increase in permeability of LPS was significantly attenuated by co-incubation with NaBut. Collectively, our results suggested that NaBut may be a potentially useful medication to prevent SOLE-induced increase in intestinal permeability of LPS.
authors.affiliation: Xin Hua Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, China; Shanghai Key Laboratory of Pediatric Gastroenterology and Nutrition, Shanghai Institute for Pediatric Research, Shanghai, China.
authors.forename: Jun-Kai
authors.initials: JK
authors.lastname: Yan
authors.affiliation: Xin Hua Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, China; Shanghai Key Laboratory of Pediatric Gastroenterology and Nutrition, Shanghai Institute for Pediatric Research, Shanghai, China.
authors.forename: Zi-Zhen
authors.initials: ZZ
authors.lastname: Gong
authors.affiliation: Xin Hua Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, China; Shanghai Key Laboratory of Pediatric Gastroenterology and Nutrition, Shanghai Institute for Pediatric Research, Shanghai, China.
authors.forename: Tian
authors.initials: T
authors.lastname: Zhang
authors.affiliation: Xin Hua Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, China; Shanghai Key Laboratory of Pediatric Gastroenterology and Nutrition, Shanghai Institute for Pediatric Research, Shanghai, China. Electronic address: caiw204@sina.com.
authors.forename: Wei
authors.initials: W
authors.lastname: Cai
mesh_terms: D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1 / Q000378:metabolism*; D020148:Butyric Acid / Q000494:pharmacology*; D018938:Caco-2 Cells; D004655:Emulsions / Q000009:adverse effects; D006801:Humans; D007413:Intestinal Mucosa / Q000378:metabolism*; D007422:Intestines / Q000187:drug effects; D008070:Lipopolysaccharides / Q000378:metabolism*; D010288:Parenteral Nutrition / Q000009:adverse effects; D010539:Permeability / Q000187:drug effects*; D013024:Soybean Oil / Q000009:adverse effects*
chemical_list: D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D004655:Emulsions; D008070:Lipopolysaccharides; D020148:Butyric Acid; D013024:Soybean Oil
keywords: Caco-2; Lipopolysaccharide; P-glycoprotein; Parenteral nutrition; Sodium butyrate; Soybean oil-based lipid emulsion