title: Flavobacterium cloacae sp. nov., isolated from waste water.
abstract: A Gram-stain-negative, non-spore-forming, rod-shaped, non-motile bacterium, named wh15T, was isolated from the waste water of a municipal wastewater treatment plant in Wuhu, PR China. The taxonomic status of the strain was characterized using a polyphasic taxonomic approach. Growth occurred at 15-45 °C (optimum, 25-40 °C), at pH 5.0-8.0 (optimum, pH 7.0) and with 0-4 % NaCl (optimum, 1-2 %). Phylogenetic analyses based on 16S rRNA gene sequences revealed that wh15T represented a member of genus Flavobacterium and shared the highest similarity with Flavobacterium ummariense DS-12T (93.93 %), Flavobacterium ceti 454-2T (93.71 %) and Flavobacterium marinum SW105T (93.63 %). The major respiratory quinone of wh15T was MK-6 and major fatty acids were iso-C15 : 0, iso-C15 : 0 3-OH, iso-C17 : 0 3-OH, summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c) and summed feature 9 (iso-C17 : 1ω9c and/or C16 : 0 10-methyl). The DNA G+C content of wh15T was 32.3 mol%. Phenotypic, phylogenetic and chemotaxonomic evidence indicated that wh15T represents a novel species of the genus Flavobacterium, the name Flavobacterium cloacae is proposed. The type strain is wh15T (=CCTCC AB 2015420T=JCM 31173T).
authors.affiliation: Institute of Molecular Biology and Biotechnology, Anhui Provincial Key Laboratory of the Conservation and Exploitation of Biological Resources, Anhui Normal University, No. 1 Beijing East Road, Wuhu 241000, Anhui, PR China.
authors.forename: Hongming
authors.initials: H
authors.lastname: Liu
authors.affiliation: Institute of Molecular Biology and Biotechnology, Anhui Provincial Key Laboratory of the Conservation and Exploitation of Biological Resources, Anhui Normal University, No. 1 Beijing East Road, Wuhu 241000, Anhui, PR China.
authors.forename: Peng
authors.initials: P
authors.lastname: Lu
authors.affiliation: Institute of Molecular Biology and Biotechnology, Anhui Provincial Key Laboratory of the Conservation and Exploitation of Biological Resources, Anhui Normal University, No. 1 Beijing East Road, Wuhu 241000, Anhui, PR China.
authors.forename: Guoping
authors.initials: G
authors.lastname: Zhu
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D002681:China; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D005417:Flavobacterium / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000737:chemistry; D062065:Wastewater / Q000382:microbiology*
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; D062065:Wastewater; C042455:menaquinone 6