title: Characterization of EBV Promoters and Coding Regions by Sequencing PCR-Amplified DNA Fragments.
abstract: DNA sequencing approaches originally developed in two directions, the chemical degradation method and the chain-termination method. The latter one became more widespread and a huge amount of sequencing data including whole genome sequences accumulated, based on the use of capillary sequencer systems and the application of a modified chain-termination method which proved to be relatively easy, fast, and reliable. In addition, relatively long, up to 1000 bp sequences could be obtained with a single read with high per-base accuracy. Although the recent appearance of next-generation DNA sequencing (NGS) technologies enabled high-throughput and low cost analysis of DNA, the modified chain-terminating methods are often applied in research until now. In the following, we shall present the application of capillary sequencing for the sequence characterization of viral genomes in case of partial and whole genome sequencing, and demonstrate it on the BARF1 promoter of Epstein Barr virus (EBV).
authors.affiliation: RT-Europe Nonprofit Research Ltd., Vár 2, Szigetköz Building, Mosonmagyaróvár, 9200, Hungary.
authors.forename: Kalman
authors.initials: K
authors.lastname: Szenthe
authors.affiliation: RT-Europe Nonprofit Research Ltd., Vár 2, Szigetköz Building, Mosonmagyaróvár, 9200, Hungary.
authors.forename: Ferenc
authors.initials: F
authors.lastname: Bánáti
mesh_terms: D002460:Cell Line; D004279:DNA, Viral; D016679:Genome, Viral; D023281:Genomics / Q000379:methods; D004854:Herpesvirus 4, Human / Q000235:genetics*; D006801:Humans; D016366:Open Reading Frames; D016133:Polymerase Chain Reaction; D011401:Promoter Regions, Genetic; D014764:Viral Proteins / Q000235:genetics; D000073336:Whole Genome Sequencing
chemical_list: C086495:BARF1 protein, Human herpesvirus 4; D004279:DNA, Viral; D014764:Viral Proteins
keywords: Capillary sequencing; EBV; Whole genome sequencing