title: Polymorphobacter glacialis sp. nov., isolated from ice core.
abstract: A Gram-stain-negative, non-spore-forming, rod-shaped bacterium, B555-2T, was isolated from an ice core drilled from Muztagh Glacier on the Tibetan Plateau, China. According to phylogenetic analysis with 16S rRNA gene sequences, the novel strain was most closely related to Polymorphobacter fuscus D40PTand Polymorphobacter multimanifer 262-7Twith 98.4 and 96.9 % similarity, respectively. It grew optimally at pH 7 and 15 °C with 0.6 % NaCl (m/v). Carotenoid was detected from the cells. Major polar lipids were phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, sphingoglycolipid, phophatidylmonomethy lethanolamine, phophatidylcholine. The major fatty acids were summed feature 3 (C16 : 1ω7c and/or iso-C15 : 0 2-OH) (42.8 %), summed feature 8 (C18 : 1ω7c and/or C18 : 1ω6c) (28.8 %), C14 : 0 2-OH (10.1 %) and C16 : 0 (8.2 %). The predominant ubiquinone was Q-10. The DNA G+C content was 62.1 mol%. In DNA-DNA hybridization tests, strain B555-2Tshared 21.9 and 18.6 % DNA-DNA relatedness with P. fuscus D40PTand P.multimanifer 262-7T, respectively. Accordingly, strain B555-2Trepresents a novel species in the genus Polymorphobacter, for which the name Polymorphobacter glacialis sp. nov. is proposed. The type strain is B555-2T(=CGMCC 1.15519T=KCTC 52396T).
authors.affiliation: Key Laboratory of Alpine Ecology and Biodiversity, Institute of Tibetan Plateau Research, Chinese Academy of Sciences, Beijing 100101, PR China.
authors.forename: Tingting
authors.initials: T
authors.lastname: Xing
authors.affiliation: Key Laboratory of Tibetan Environment Changes and Land Surface Processes, Institute of Tibetan Plateau Research, Chinese Academy of Sciences, Beijing 100101, PR China.|CAS Center for Excellence in Tibetan Plateau Earth Sciences, Chinese Academy of Sciences, Beijing 100101, PR China.
authors.forename: Yongqin
authors.initials: Y
authors.lastname: Liu
authors.affiliation: College of Urban and Environmental Science, Northwest University, Xi'an 710069, PR China.|CAS Center for Excellence in Tibetan Plateau Earth Sciences, Chinese Academy of Sciences, Beijing 100101, PR China.
authors.forename: Ninglian
authors.initials: N
authors.lastname: Wang
authors.affiliation: Key Laboratory of Tibetan Environment Changes and Land Surface Processes, Institute of Tibetan Plateau Research, Chinese Academy of Sciences, Beijing 100101, PR China.|CAS Center for Excellence in Tibetan Plateau Earth Sciences, Chinese Academy of Sciences, Beijing 100101, PR China.
authors.forename: Baiqing
authors.initials: B
authors.lastname: Xu
authors.affiliation: Key Laboratory of Alpine Ecology and Biodiversity, Institute of Tibetan Plateau Research, Chinese Academy of Sciences, Beijing 100101, PR China.
authors.forename: Liang
authors.initials: L
authors.lastname: Shen
authors.affiliation: Key Laboratory of Alpine Ecology and Biodiversity, Institute of Tibetan Plateau Research, Chinese Academy of Sciences, Beijing 100101, PR China.
authors.forename: Keshao
authors.initials: K
authors.lastname: Liu
authors.affiliation: Key Laboratory of Alpine Ecology and Biodiversity, Institute of Tibetan Plateau Research, Chinese Academy of Sciences, Beijing 100101, PR China.
authors.forename: Zhengquan
authors.initials: Z
authors.lastname: Gu
authors.affiliation: Key Laboratory of Alpine Ecology and Biodiversity, Institute of Tibetan Plateau Research, Chinese Academy of Sciences, Beijing 100101, PR China.
authors.forename: Bixi
authors.initials: B
authors.lastname: Guo
authors.affiliation: Institute of Microbiology, China General Microbiological Culture Collection Center, Chinese Academy of Sciences, Beijing 100101, PR China.
authors.forename: Yuguang
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: Institute of Microbiology, China General Microbiological Culture Collection Center, Chinese Academy of Sciences, Beijing 100101, PR China.
authors.forename: Hongcan
authors.initials: H
authors.lastname: Liu
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D002338:Carotenoids / Q000737:chemistry; D002681:China; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D046448:Ice Cover / Q000382:microbiology*; D009693:Nucleic Acid Hybridization; D010743:Phospholipids / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D042301:Sphingomonadaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D014451:Ubiquinone / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D014451:Ubiquinone; D002338:Carotenoids; C025204:Ubiquinone Q2
keywords: Polymorphobacter; glacialis