title: Association of HFE gene mutations with nonalcoholic fatty liver disease in the Iranian population.
abstract: To determine whether the HFE gene variants H63D and C282Y are associated with NAFLD in persons with type 2 diabetes, we conducted a case-control study including 145 case of NAFLD patients with a history of type 2 diabetes and 145 matching control. The genomic DNA was extracted from the peripheral venous blood and the genotyping of HFE gene mutations was analyzed using the PCR-RFLP technique. Statistical analysis was performed using SPSS 12.0 software by χ2 test, t test and ANOVA (P&lt;0.05). Data showed no increased frequency of HFE mutations in persons with type 2 diabetes and no association between H63D mutation and NAFLD in the study population. Also, we analyzed index of physiological variables including FBS, lipid profile (TC, TG, LDL-C, and HDL-C), BMI, HbA1c, and micro albuminuria and Cr levels). Data showed there are no relationship between these indexes and HFE gene mutations and either NAFLD as a complication of diabetes. But our results showed a relationship between C282Y mutation and NAFLD in persons with type 2 diabetes. C282Y mutation might be a genetic marker of NAFLD in Iranian population.
authors.affiliation: Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran.
authors.forename: L
authors.initials: L
authors.lastname: Saremi
authors.affiliation: Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran.
authors.forename: S
authors.initials: S
authors.lastname: Lotfipanah
authors.affiliation: Baqiyatallah University of Medical Sciences, Tehran, Iran.
authors.forename: M
authors.initials: M
authors.lastname: Mohammadi
authors.affiliation: Department of Biology, Faculty of Science, Yazd University, Yazd, Iran.
authors.forename: H
authors.initials: H
authors.lastname: Hosseinzadeh
authors.affiliation: Department of Medical Genetics, Shahid Beheshti University of Medical Science, Tehran, Iran.
authors.forename: A
authors.initials: A
authors.lastname: Sayad
authors.affiliation: Department of Molecular Medicine, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran. z-saltanatpour@razi.tums.ac.ir.
authors.forename: Z
authors.initials: Z
authors.lastname: Saltanatpour
mesh_terms: D000483:Alleles; D044466:Asian People / Q000235:genetics*; D015992:Body Mass Index; D016022:Case-Control Studies; D003924:Diabetes Mellitus, Type 2 / Q000150:complications; D005787:Gene Frequency; D005838:Genotype; D006442:Glycated Hemoglobin / Q000032:analysis; D000071020:Hemochromatosis Protein / Q000235:genetics*; D006801:Humans; D007501:Iron / Q000378:metabolism; D008055:Lipids / Q000097:blood; D065626:Non-alcoholic Fatty Liver Disease / Q000150:complications / Q000235:genetics* / Q000473:pathology; D016017:Odds Ratio; D020641:Polymorphism, Single Nucleotide
chemical_list: D006442:Glycated Hemoglobin; D000071020:Hemochromatosis Protein; D007501:Iron; D008055:Lipids; C103673:HFE protein, human