title: Beggiatoa leptomitoformis sp. nov., the first freshwater member of the genus capable of chemolithoautotrophic growth.
abstract: A strain of filamentous sulfur bacteria was isolated from freshwater spring contaminated with residential and agricultural wastewater in Moscow region, Russia. According to the results of phylogenetic analysis, strain D-402T belonged to the genus Beggiatoa within the family Beggiatoaceae of the class Gammaproteobacteria. Within the genus Beggiatoa, strain D-402T was most closely related to Beggiatoa alba strains. Strain D-402T had a DNA G+C content 42.1 mol%. The DNA-DNA hybridization value between strain D-402T and Beggiatoa alba strain B15LD was 33 %. Predominant fatty acids were C18 : 1 (46.1 and 53.3 %), C16 : 0 (15.5 and 16.2 %) and C16 : 1 (32.9 and 25.4 %) for strains D-402T and B15LD, respectively. In contrast to known representatives of Beggiatoa, strain D-402T was capable of chemolithoautotrophic growth with sulfide and thiosulfate as electron donors. Oxidation of sulfide and thiosulfate was accompanied by deposition of sulfur globules within the cells. Strain D-402T was capable of heterotrophic growth. The strain was capable of using different organic compounds, sulfur compounds and hydrogen as electron donors. Based on these observations, strain D-402T is considered as a representative of a species Beggiatoa leptomitoformis sp. nov. of the genus Beggiatoa. The type strain is D-402T (=DSM 14946T=UNIQEM U 779T).
authors.affiliation: Federal State Institution 'Federal Research Centre "Fundamentals of Biotechnology" of the Russian Academy of Sciences', Prospect 60-letiya Oktyabrya, 7/2, 117312 Moscow, Russia.
authors.forename: Galina
authors.initials: G
authors.lastname: Dubinina
authors.affiliation: Federal State Institution 'Federal Research Centre "Fundamentals of Biotechnology" of the Russian Academy of Sciences', Prospect 60-letiya Oktyabrya, 7/2, 117312 Moscow, Russia.
authors.forename: Alexander
authors.initials: A
authors.lastname: Savvichev
authors.affiliation: Department of Biochemistry and Cell Physiology, Voronezh State University, Universitetskaya pl., 1, Voronezh 394018, Russia.
authors.forename: Maria
authors.initials: M
authors.lastname: Orlova
authors.affiliation: Arietis Pharma, Boston, MA 02118, USA.
authors.forename: Ekaterina
authors.initials: E
authors.lastname: Gavrish
authors.affiliation: Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures, Inhoffenstraße 7B, 38124 Braunschweig, Germany.
authors.forename: Suzanne
authors.initials: S
authors.lastname: Verbarg
authors.affiliation: Department of Biochemistry and Cell Physiology, Voronezh State University, Universitetskaya pl., 1, Voronezh 394018, Russia.
authors.forename: Margarita
authors.initials: M
authors.lastname: Grabovich
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D053524:Beggiatoa / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D052837:Chemoautotrophic Growth; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D005618:Fresh Water / Q000382:microbiology*; D009693:Nucleic Acid Hybridization; D010084:Oxidation-Reduction; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012426:Russia; D017422:Sequence Analysis, DNA; D013440:Sulfides / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D013440:Sulfides