title: Polyanion-tobramycin nanocomplexes into functional microparticles for the treatment of Pseudomonas aeruginosa infections in cystic fibrosis.
abstract: OBJECTIVE
  Efficacy of antibiotics in cystic fibrosis (CF) is compromised by the poor penetration through mucus barrier. This work proposes a new 'nano-into-micro' approach, used to obtain a combinatorial effect: achieve a sustained delivery of tobramycin and overcome mucus barrier.
  
  
  METHODS
  Mannitol microparticles (MPs) were loaded with a tobramycin polymeric nanocomplex and characterized in presence of CF artificial mucus.
  
  
  CONCLUSIONS
  MPs are able to alter the rheological properties of CF artificial mucus, enhancing drug penetration into it and allowing a prolonged drug release. MPs resulted to be effective in Pseudomonas aeruginosa infections if compared with free tobramycin.
  
  
  CONCLUSIONS
  MPs resulted to be a formulation of higher efficacy, with potential positive implications, as lower required dose, administration frequency, side effects and antibiotic resistance problems.
authors.affiliation: Dipartimento di Scienze e Tecnologie Biologiche Chimiche e Farmaceutiche (STEBICEF), Università di Palermo, Via Archirafi 32, 90123 Palermo, Italy.
authors.forename: Emanuela F
authors.initials: EF
authors.lastname: Craparo
authors.affiliation: Dipartimento di Scienze e Tecnologie Biologiche Chimiche e Farmaceutiche (STEBICEF), Università di Palermo, Via Archirafi 32, 90123 Palermo, Italy.
authors.forename: Barbara
authors.initials: B
authors.lastname: Porsio
authors.affiliation: Dipartimento di Scienze e Tecnologie Biologiche Chimiche e Farmaceutiche (STEBICEF), Università di Palermo, Via Archirafi 32, 90123 Palermo, Italy.
authors.forename: Domenico
authors.initials: D
authors.lastname: Schillaci
authors.affiliation: Dipartimento di Scienze e Tecnologie Biologiche Chimiche e Farmaceutiche (STEBICEF), Università di Palermo, Via Archirafi 32, 90123 Palermo, Italy.
authors.forename: Maria G
authors.initials: MG
authors.lastname: Cusimano
authors.affiliation: Institute of Biophysics, National Research Council, Palermo, Italy.|Department of Physics & Chemistry, University of Palermo, Palermo, Italy.
authors.forename: Dario
authors.initials: D
authors.lastname: Spigolon
authors.affiliation: Dipartimento di Scienze e Tecnologie Biologiche Chimiche e Farmaceutiche (STEBICEF), Università di Palermo, Via Archirafi 32, 90123 Palermo, Italy.
authors.forename: Gaetano
authors.initials: G
authors.lastname: Giammona
authors.affiliation: Dipartimento di Scienze e Tecnologie Biologiche Chimiche e Farmaceutiche (STEBICEF), Università di Palermo, Via Archirafi 32, 90123 Palermo, Italy.
authors.forename: Gennara
authors.initials: G
authors.lastname: Cavallaro
mesh_terms: D000900:Anti-Bacterial Agents / Q000008:administration & dosage / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology*; D002460:Cell Line; D003550:Cystic Fibrosis / Q000150:complications*; D004337:Drug Carriers; D065546:Drug Liberation; D004847:Epithelial Cells / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D008353:Mannitol / Q000737:chemistry; D009093:Mucus; D053761:Nanocomposites / Q000737:chemistry; D000071228:Polyelectrolytes; D011108:Polymers / Q000138:chemical synthesis; D011552:Pseudomonas Infections / Q000188:drug therapy* / Q000209:etiology; D011550:Pseudomonas aeruginosa / Q000187:drug effects*; D014031:Tobramycin / Q000008:administration & dosage / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology*
chemical_list: D000900:Anti-Bacterial Agents; D004337:Drug Carriers; D008353:Mannitol; D000071228:Polyelectrolytes; D011108:Polymers; D014031:Tobramycin
keywords: Pseudomonas aeruginosa infections; cystic fibrosis artificial mucus; mannitol; microparticles; nano into micro strategy; spray dryer; tobramycin; α,β-poly(N-2-hydroxyethyl)-D,L-aspartamide