title: DNA Extraction Systematics for Spectroscopic Studies.
abstract: Study of genetic material allows the comprehension the origin of the many biochemical changes that follow diseases, like cancer, promoting the development of early preventive inquiry and more efficient individual treatments. Raman spectroscopy can be an important tool in DNA study, since it allows probe molecular vibrations of genetic material in a fast way. The present work established a systematic way for extract DNA in suitable concentrations and structural integrity allowing studies by Raman spectroscopy or other spectroscopic technique, including bio-analytical sensors for probing genetic alterations.
authors.affiliation: Instituto de Pesquisa & Desenvolvimento, Universidade do Vale do Paraíba, Av. Shishima Hifumi, 2911, 12244-000, São José dos Campos, São Paulo, Brazil. bianca_palma@hotmail.com.
authors.forename: Bianca Fogazza
authors.initials: BF
authors.lastname: Palma
authors.affiliation: Instituto de Pesquisa & Desenvolvimento, Universidade do Vale do Paraíba, Av. Shishima Hifumi, 2911, 12244-000, São José dos Campos, São Paulo, Brazil. amanda.borges@univap.br.
authors.forename: Amanda Borges
authors.initials: AB
authors.lastname: Ferrari
authors.affiliation: Instituto de Pesquisa & Desenvolvimento, Universidade do Vale do Paraíba, Av. Shishima Hifumi, 2911, 12244-000, São José dos Campos, São Paulo, Brazil. rabc@univap.br.
authors.forename: Renata Andrade
authors.initials: RA
authors.lastname: Bitar
authors.affiliation: Instituto de Pesquisa & Desenvolvimento, Universidade do Vale do Paraíba, Av. Shishima Hifumi, 2911, 12244-000, São José dos Campos, São Paulo, Brazil. magcard@univap.br.
authors.forename: Maria Angélica Gargione
authors.initials: MA
authors.lastname: Cardoso
authors.affiliation: Instituto de Pesquisa & Desenvolvimento, Universidade do Vale do Paraíba, Av. Shishima Hifumi, 2911, 12244-000, São José dos Campos, São Paulo, Brazil. amartin@univap.br.
authors.forename: Airton A
authors.initials: AA
authors.lastname: Martin
authors.affiliation: Centro de Ciências Naturais e Humanas, Universidade Federal do ABC, Rua Catequese 242, 09090-400, Santo André, São Paulo, Brazil. herculano.martinho@ufabc.edu.br.
authors.forename: Herculano
authors.initials: H
authors.lastname: Da Silva Martinho
keywords: Cancer.; DNA; Raman spectroscopy