title: MBTH: A novel approach to rapid, spectrophotometric quantitation of total algal carbohydrates.
abstract: A high-throughput and robust application of the 3-methyl-2-benzothiazolinone hydrazone (MBTH) method was developed for carbohydrate determination in microalgae. The traditional phenol-sulfuric acid method to quantify carbohydrates is strongly affected by algal biochemical components and exhibits a highly variable response to microalgal monosaccharides. We present a novel use of the MBTH method to accurately quantify carbohydrates in hydrolyzate after acid hydrolysis of algal biomass, without a need for neutralization. The MBTH method demonstrated consistent and sensitive quantitation of algae-specific monosaccharides down to 5 μg mL-1without interference from other algae acidic hydrolyzate components.
authors.affiliation: National Renewable Energy Laboratory, Golden, CO, USA.
authors.forename: Stefanie
authors.initials: S
authors.lastname: Van Wychen
authors.affiliation: National Renewable Energy Laboratory, Golden, CO, USA.
authors.forename: William
authors.initials: W
authors.lastname: Long
authors.affiliation: National Renewable Energy Laboratory, Golden, CO, USA.
authors.forename: Stuart K
authors.initials: SK
authors.lastname: Black
authors.affiliation: National Renewable Energy Laboratory, Golden, CO, USA. Electronic address: Lieve.Laurens@nrel.gov.
authors.forename: Lieve M L
authors.initials: LM
authors.lastname: Laurens
mesh_terms: D052160:Benzothiazoles / Q000737:chemistry*; D050260:Carbohydrate Metabolism / Q000502:physiology; D002241:Carbohydrates / Q000037:antagonists & inhibitors / Q000737:chemistry*; D048408:Chlorella vulgaris / Q000737:chemistry* / Q000378:metabolism; D006835:Hydrazones / Q000737:chemistry*; D006868:Hydrolysis; D044444:Scenedesmus / Q000737:chemistry* / Q000378:metabolism; D013053:Spectrophotometry / Q000379:methods
chemical_list: D052160:Benzothiazoles; D002241:Carbohydrates; D006835:Hydrazones; C002536:3-methyl-2-benzothiazolone hydrazone
keywords: Algae; Carbohydrates; MBTH; Phenol-sulfuric acid; Spectrophotometric