title: Novel Substrates for Microarrays.
abstract: In the microarray platform, the surface substrate is critical to the result quality in terms of signal consistency and detection sensitivity. Traditional substrates such as glass and nitrocellulose often entail complicated preparation processes such as the activation and functionalization of the reaction spots and surface blocking to prevent nonspecific molecule adsorption. In addition, coffee-ring morphology of the spots is a common issue in the traditional substrates. To address these issues, we introduced a novel substrate based on fluorinated ethylene propylene (FEP) membrane for microarrays.
authors.affiliation: Department of Chemistry, The Chinese University of Hong Kong, Hong Kong, People's Republic of China.
authors.forename: Dameng
authors.initials: D
authors.lastname: Guo
authors.affiliation: Department of Chemistry, The Chinese University of Hong Kong, Hong Kong, People's Republic of China.
authors.forename: Han
authors.initials: H
authors.lastname: Wu
authors.affiliation: Department of Chemistry, The Chinese University of Hong Kong, Hong Kong, People's Republic of China.
authors.forename: Liang
authors.initials: L
authors.lastname: Wu
authors.affiliation: Department of Chemistry, The Chinese University of Hong Kong, Hong Kong, People's Republic of China. bozheng@cuhk.edu.hk.
authors.forename: Bo
authors.initials: B
authors.lastname: Zheng
mesh_terms: D000906:Antibodies / Q000032:analysis; D016207:Cytokines / Q000032:analysis; D004797:Enzyme-Linked Immunosorbent Assay; D007211:Indoles / Q000737:chemistry; D046228:Microarray Analysis / Q000379:methods*; D010455:Peptides / Q000032:analysis; D011108:Polymers / Q000737:chemistry; D011138:Polytetrafluoroethylene / Q000031:analogs & derivatives; D040081:Protein Array Analysis; D013379:Substrate Specificity; D005465:Fluorocarbon Polymers
chemical_list: D000906:Antibodies; D016207:Cytokines; D007211:Indoles; D010455:Peptides; D011108:Polymers; D011138:Polytetrafluoroethylene; C096305:fluorinated ethylene propylene; D005465:Fluorocarbon Polymers; C568283:polydopamine
keywords: Fluorinated ethylene propylene; Microarray substrate; Peptide microarray; Polydopamine; Protein microarray; Sandwich ELISA; Teflon