title: Estimating total lipid content of Camelina sativa via pyrolysis assisted in-situ transesterification with dimethyl carbonate.
abstract: Direct derivatization of C. sativa seed into FAMEs without lipid extraction was conducted for the quantification of lipid analysis via in-situ thermal methylation with dimethyl carbonate as an acyl acceptor on silica (SiO2). The introduced method had an extraordinarily high tolerance against impurities such as pyrolytic products and moisture. To ensure the technical completeness of in-situ methylation, thermal cracking of FAMEs transformed from C. sativa seed was also explored. Thermal cracking of unsaturated FAMEs such as C18:1, C18:2, C18:3and C20:1occurred at temperatures higher than 365°C due to their thermal instability. Thus, experimental findings in this study suggests not only that qualitative analysis of fatty acid profile in C. sativa seed via in-situ methylation using SiO2could be achieve, but also that the total lipid content (42.65wt.%) in C. sativa seed could be accurately estimated.
authors.affiliation: Department of Environment and Energy, Sejong University, Seoul 05006, Republic of Korea.
authors.forename: Jong-Min
authors.initials: JM
authors.lastname: Jung
authors.affiliation: Department of Environment and Energy, Sejong University, Seoul 05006, Republic of Korea.
authors.forename: Jechan
authors.initials: J
authors.lastname: Lee
authors.affiliation: Advanced Technology Department, Land & Housing Institute, Daejeon 34047, Republic of Korea.
authors.forename: Jeong-Ik
authors.initials: JI
authors.lastname: Oh
authors.affiliation: College of Life Sciences, Sejong University, Seoul 05006, Republic of Korea. Electronic address: kimhyung@sejong.ac.kr.
authors.forename: Hyung-Wook
authors.initials: HW
authors.lastname: Kim
authors.affiliation: Department of Environment and Energy, Sejong University, Seoul 05006, Republic of Korea.
authors.forename: Eilhann E
authors.initials: EE
authors.lastname: Kwon
mesh_terms: D001671:Biochemistry / Q000379:methods*; D019607:Brassicaceae / Q000737:chemistry*; D004951:Esterification; D005227:Fatty Acids / Q000032:analysis; D005561:Formates / Q000737:chemistry*; D008055:Lipids / Q000032:analysis* / Q000737:chemistry; D012639:Seeds / Q000737:chemistry; D013696:Temperature; D014280:Triglycerides / Q000032:analysis / Q000737:chemistry
chemical_list: D005227:Fatty Acids; D005561:Formates; D008055:Lipids; D014280:Triglycerides; C023025:methyl carbonate
keywords: Camelina sativa; Derivatization; Dimethyl carbonate; Fatty acid methyl esters; Triglycerides