title: Rhodococcus sovatensis sp. nov., an actinomycete isolated from the hypersaline and heliothermal Lake Ursu.
abstract: A Gram-stain-positive, strictly aerobic, mesophilic bacterium, designated H004T, was isolated from a water sample of the hypersaline and heliothermal Lake Ursu, Sovata, Romania. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain H004T formed a distinct phyletic lineage within the genus Rhodococcus. It shared the highest 16S rRNA gene sequence similarity with Rhodococcus yunnanensis YIM 70056T (98.80 %), followed by Rhodococcus fascians LMG 3623T (98.73 %), Rhodococcus cercidiphylli YIM 65003T (98.73 %), Rhodococcus cerastii C5T (98.58 %) and Rhodococcus kyotonensis DS472T (98.53 %). The alkB-based phylogenetic analysis further confirmed that this strain constitutes a highly unique lineage within the genus. Chemotaxonomic characteristics, including the predominant fatty acids acids C15 : 0, C18 : 1ω9c, C19 : 1ω11c/C19 : 1ω9c and C16 : 1ω7c/iso-C15 : 0 2-OH, the major quinone MK-8(H2), the presence of mycolic acids and cell-wall chemotype IV were also consistent with the properties of members of the genus Rhodococcus. The DNA G+C content of strain H004T was 65.4 mol%. The results of DNA-DNA hybridization analyses with the closest relatives, in combination with the alkB-based phylogenetic analysis, as well as the chemotaxonomic and physiological data, demonstrated that isolate H004T represents a novel species of the genus Rhodococcus, for which the name Rhodococcus sovatensissp. nov. is proposed. The type strain is H004T (=DSM 102881T=NCAIM B.02632T).
authors.affiliation: Regional University Center of Excellence in Environmental Industry, Szent István University, Páter K. u. 1, H-2100 Gödöllő, Hungary.
authors.forename: András
authors.initials: A
authors.lastname: Táncsics
authors.affiliation: Department of Bioengineering, Sapientia Hungarian University of Transylvania, Piaţa Libertăţii 1, R-530104, Miercurea Ciuc, Romania.
authors.forename: István
authors.initials: I
authors.lastname: Máthé
authors.affiliation: Regional University Center of Excellence in Environmental Industry, Szent István University, Páter K. u. 1, H-2100 Gödöllő, Hungary.
authors.forename: Tibor
authors.initials: T
authors.lastname: Benedek
authors.affiliation: Department of Microbiology, Eötvös Loránd University, Pázmány P. sétány 1/C, H-1117 Budapest, Hungary.
authors.forename: Erika M
authors.initials: EM
authors.lastname: Tóth
authors.affiliation: DSMZ - German Collection of Microorganisms and Cell Cultures, Inhoffenstrasse 7B, D-38124, Braunschweig, Germany.
authors.forename: Ewelina
authors.initials: E
authors.lastname: Atasayar
authors.affiliation: DSMZ - German Collection of Microorganisms and Cell Cultures, Inhoffenstrasse 7B, D-38124, Braunschweig, Germany.
authors.forename: Cathrin
authors.initials: C
authors.lastname: Spröer
authors.affiliation: Department of Microbiology, Eötvös Loránd University, Pázmány P. sétány 1/C, H-1117 Budapest, Hungary.
authors.forename: Károly
authors.initials: K
authors.lastname: Márialigeti
authors.affiliation: Department of Microbiology, Eötvös Loránd University, Pázmány P. sétány 1/C, H-1117 Budapest, Hungary.|Department of Bioengineering, Sapientia Hungarian University of Transylvania, Piaţa Libertăţii 1, R-530104, Miercurea Ciuc, Romania.
authors.forename: Tamás
authors.initials: T
authors.lastname: Felföldi
authors.affiliation: Department of Environmental Protection and Environmental Safety, Szent István University, Páter K. u. 1, H-2100 Gödöllő, Hungary.
authors.forename: Balázs
authors.initials: B
authors.lastname: Kriszt
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D060106:Lakes / Q000382:microbiology*; D009171:Mycolic Acids / Q000737:chemistry; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012240:Rhodococcus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D012383:Romania; D054712:Salinity; D017422:Sequence Analysis, DNA
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D009171:Mycolic Acids; D012336:RNA, Ribosomal, 16S