title: Improvement in routine detection of colistin resistance in E. coli isolated in veterinary diagnostic laboratories.
abstract: We have developed a phenotypic method suited to the systematic screening of resistance to colistin in E. coli, including those with the mcr-1 gene, by the absence of an inhibition zone after an application of a single drop of 8mg/L colistin solution on a previously inoculated Mueller-Hinton agar.
authors.affiliation: ANSES, Laboratoire de Ploufragan-Plouzané, Unité Mycoplasmologie-Bactériologie, Ploufragan, France; Univ Bretagne Loire, France. Electronic address: eric.jouy@anses.fr.
authors.forename: Eric
authors.initials: E
authors.lastname: Jouy
authors.affiliation: ANSES, Laboratoire de Lyon, Unité Antibiorésistance et Virulence Bactérienne, Lyon, France.
authors.forename: Marisa
authors.initials: M
authors.lastname: Haenni
authors.affiliation: ANSES, Laboratoire de Ploufragan-Plouzané, Unité Mycoplasmologie-Bactériologie, Ploufragan, France; Univ Bretagne Loire, France.
authors.forename: Laëtitia
authors.initials: L
authors.lastname: Le Devendec
authors.affiliation: ANSES, Laboratoire de Ploufragan-Plouzané, Unité Mycoplasmologie-Bactériologie, Ploufragan, France; Univ Bretagne Loire, France.
authors.forename: Aurélie
authors.initials: A
authors.lastname: Le Roux
authors.affiliation: ANSES, Laboratoire de Lyon, Unité Antibiorésistance et Virulence Bactérienne, Lyon, France.
authors.forename: Pierre
authors.initials: P
authors.lastname: Châtre
authors.affiliation: ANSES, Laboratoire de Lyon, Unité Antibiorésistance et Virulence Bactérienne, Lyon, France.
authors.forename: Jean-Yves
authors.initials: JY
authors.lastname: Madec
authors.affiliation: ANSES, Laboratoire de Ploufragan-Plouzané, Unité Mycoplasmologie-Bactériologie, Ploufragan, France; Univ Bretagne Loire, France. Electronic address: isabelle.kempf@anses.fr.
authors.forename: Isabelle
authors.initials: I
authors.lastname: Kempf
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D003091:Colistin / Q000494:pharmacology*; D024881:Drug Resistance, Bacterial; D004926:Escherichia coli / Q000187:drug effects*; D008826:Microbial Sensitivity Tests
chemical_list: D000900:Anti-Bacterial Agents; D003091:Colistin
keywords: Colistin resistance; E. coli; Phenotypic detection; mcr-1