title: miR-379 Inhibits Cell Proliferation, Invasion, and Migration of Vascular Smooth Muscle Cells by Targeting Insulin-Like Factor-1.
abstract: OBJECTIVE
  MicroRNAs are small non-coding RNAs that play important roles in vascular smooth muscle cell (VSMC) function. This study investigated the role of miR-379 on proliferation, invasion, and migration of VSMCs and explored underlying mechanisms thereof.
  
  
  METHODS
  MicroRNA, mRNA, and protein levels were determined by quantitative real-time PCR and western blot. The proliferative, invasive, and migratory abilities of VSMCs were measured by CCK-8, invasion, and wound healing assay, respectively. Luciferase reporter assay was used to confirm the target of miR-379.
  
  
  RESULTS
  Platelet-derived growth factor-bb was found to promote cell proliferation and suppress miR-379 expression in VSMCs. Functional assays demonstrated that miR-379 inhibited cell proliferation, cell invasion, and migration. Flow cytometry results further showed that miR-379 induced apoptosis in VSMCs. TargetScan analysis and luciferase report assay confirmed that insulin-like growth factor-1 (IGF-1) 3'UTR is a direct target of miR-379, and mRNA and protein levels of miR-379 and IGF-1 were inversely correlated. Rescue experiments showed that enforced expression of IGF-1 sufficiently overcomes the inhibitory effect of miR-379 on cell proliferation, invasion, and migration in VSMCs.
  
  
  CONCLUSIONS
  Our results suggest that miR-379 plays an important role in regulating VSMCs proliferation, invasion, and migration by targeting IGF-1.
authors.affiliation: Department of Cardiology, The First Affiliated Hospital of Xi'an Medical University, Xi'an, Shaanxi Province, China.
authors.forename: Kai
authors.initials: K
authors.lastname: Li
authors.affiliation: Department of Cardiology, The First Affiliated Hospital of Xi'an Medical University, Xi'an, Shaanxi Province, China. yong_wang0756@sina.com.
authors.forename: Yong
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Cardiology, The First Affiliated Hospital of Xi'an Medical University, Xi'an, Shaanxi Province, China.
authors.forename: Anji
authors.initials: A
authors.lastname: Zhang
authors.affiliation: Department of Cardiology, The First Affiliated Hospital of Xi'an Medical University, Xi'an, Shaanxi Province, China.
authors.forename: Baixue
authors.initials: B
authors.lastname: Liu
authors.affiliation: Department of Cardiology, The First Affiliated Hospital of Xi'an Medical University, Xi'an, Shaanxi Province, China.
authors.forename: Li
authors.initials: L
authors.lastname: Jia
mesh_terms: D017209:Apoptosis; D000077214:Becaplermin; D002465:Cell Movement / Q000502:physiology*; D049109:Cell Proliferation / Q000502:physiology*; D006801:Humans; D007328:Insulin; D007334:Insulin-Like Growth Factor I / Q000502:physiology*; D035683:MicroRNAs / Q000502:physiology*; D009131:Muscle, Smooth, Vascular / Q000166:cytology*; D020574:Proto-Oncogene Proteins c-sis / Q000502:physiology*; D012333:RNA, Messenger / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D012844:Sincalide / Q000502:physiology; D014945:Wound Healing / Q000502:physiology
chemical_list: D007328:Insulin; C561022:MIRN379 microRNA, human; D035683:MicroRNAs; D020574:Proto-Oncogene Proteins c-sis; D012333:RNA, Messenger; D000077214:Becaplermin; D007334:Insulin-Like Growth Factor I; D012844:Sincalide
keywords: IGF-1; Vascular smooth muscle cells; cell proliferation; invasion; miR-379; migration