title: Pleomorphochaeta caudata gen. nov., sp. nov., an anaerobic bacterium isolated from an offshore oil well, reclassification of Sphaerochaeta multiformis MO-SPC2T as Pleomorphochaeta multiformis MO-SPC2T comb. nov. as the type strain of this novel genus and emended description of the genus Sphaerochaeta.
abstract: A strictly anaerobic Gram-stain-negative bacterium, designated strain SEBR 4223T, was isolated from the production water of an offshore Congolese oil field. Cells were non-motile, pleomorphic and had spherical, annular or budding shapes, often exhibiting long stalks. Strain SEBR 4223T grew on a range of carbohydrates, optimally at 37 °C and pH 7, in a medium containing 40 g l-1 NaCl. Predominant fatty acids were C14 : 0, C14 : 0 DMA, C16 : 0 and C16 : 1ω7c and the major polar lipids were phosphoglycolipids, phospholipids, glycolipids and diphosphatidylglycerol. The G+C content of the DNA was 28.7 mol%. Phylogenetic analysis, based on the 16S rRNA gene sequence, showed that strain SEBR 4223T and Sphaerochaeta multiformis MO-SPC2T formed a cluster with similarity to other species of the genus Sphaerochaeta of of less than 86 %. On the basis of the phenotypic characteristics and taxonomic analyses, we propose a novel genus, Pleomorphochaeta gen. nov., to accommodate the novel species Pleomorphochaeta caudata sp. nov., with SEBR 4223T (=DSM 103077T=JCM 31 475T) as the type strain. We also propose the reclassification of Sphaerochaeta multiformis MO SPC2T as Pleomorphochaeta multiformis MO-SPC2T comb. nov., the type strain of this novel genus and emend description of the genus Sphaerochaeta.
authors.affiliation: CNRS/ Université de Pau & des Pays de l'Adour, IPREM UMR 5254, 64000, Pau, France.
authors.forename: Boussad
authors.initials: B
authors.lastname: Arroua
authors.affiliation: CNRS/ Université de Pau & des Pays de l'Adour, IPREM UMR 5254, 64000, Pau, France.
authors.forename: Anthony
authors.initials: A
authors.lastname: Ranchou-Peyruse
authors.affiliation: CNRS/ Université de Pau & des Pays de l'Adour, IPREM UMR 5254, 64000, Pau, France.
authors.forename: Magali
authors.initials: M
authors.lastname: Ranchou-Peyruse
authors.affiliation: CNRS/ Université de Pau & des Pays de l'Adour, IPREM UMR 5254, 64000, Pau, France.
authors.forename: Michel
authors.initials: M
authors.lastname: Magot
authors.affiliation: CNRS/ Université de Pau & des Pays de l'Adour, IPREM UMR 5254, 64000, Pau, France.
authors.forename: Laurent
authors.initials: L
authors.lastname: Urios
authors.affiliation: CNRS/ Université de Pau & des Pays de l'Adour, IPREM UMR 5254, 64000, Pau, France.
authors.forename: Régis
authors.initials: R
authors.lastname: Grimaud
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D003223:Congo; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D006017:Glycolipids / Q000737:chemistry; D061329:Oil and Gas Fields / Q000382:microbiology*; D010743:Phospholipids / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012623:Seawater / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D013143:Spirochaetaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D006017:Glycolipids; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S