title: The Use of Chromatin Precipitation Coupled to DNA Sequencing (ChIP-Seq) for the Analysis of Zta Binding to the Human and EBV Genome.
abstract: Determining which components of the transcription machinery associate with the viral and cellular genome, and how this changes at specific stages of the viral life cycle is paramount to understanding how the distinct transcriptional programs associated with primary infection, latency, and disease are established and how they are reprogrammed during initiation and execution of the viral lytic replication cycle. Chromatin precipitations linked to next generation DNA sequencing (ChIP-Seq) allow for the interactions of proteins with DNA to be mapped across both viral and cellular genomes. This can be applied to viral and cellular transcription factors, coactivators and corepressors, modified histones, and modulators of chromatin.
authors.affiliation: School of Life Sciences, University of Sussex, JMS Bldg 3C19, Brighton, BN1 9QG, UK.
authors.forename: Anja
authors.initials: A
authors.lastname: Godfrey
authors.affiliation: V ClinBio Labs Pvt Ltd., Central Research Facility, Sri Ramachandra University, Porur, Chennai, 600 016, India.
authors.forename: Sharada
authors.initials: S
authors.lastname: Ramasubramanyan
authors.affiliation: School of Life Sciences, University of Sussex, JMS Bldg 3C19, Brighton, BN1 9QG, UK. A.J.Sinclair@sussex.ac.uk.
authors.forename: Alison J
authors.initials: AJ
authors.lastname: Sinclair
mesh_terms: D001665:Binding Sites; D047369:Chromatin Immunoprecipitation; D015894:Genome, Human; D016679:Genome, Viral; D004854:Herpesvirus 4, Human / Q000235:genetics*; D059014:High-Throughput Nucleotide Sequencing; D054884:Host-Pathogen Interactions; D006801:Humans; D011485:Protein Binding; D015534:Trans-Activators / Q000378:metabolism*; D014157:Transcription Factors / Q000378:metabolism
chemical_list: C063676:BZLF1 protein, Herpesvirus 4, Human; D015534:Trans-Activators; D014157:Transcription Factors
keywords: ChIP; Chromatin; DNA; DNA sequencing; Immunoprecipitation; Transcription factor