title: Compound danshen dripping pills modulate the perturbed energy metabolism in a rat model of acute myocardial ischemia.
abstract: The continuous administration of compound danshen dripping pills (CDDP) showed good efficacy in relieving myocardial ischemia clinically. To probe the underlying mechanism, metabolic features were evaluated in a rat model of acute myocardial ischemia induced by isoproterenol (ISO) and administrated with CDDP using a metabolomics platform. Our data revealed that the ISO-induced animal model showed obvious myocardial injury, decreased energy production, and a marked change in metabolomic patterns in plasma and heart tissue. CDDP pretreatment increased energy production, ameliorated biochemical indices, modulated the changes and metabolomic pattern induced by ISO, especially in heart tissue. For the first time, we found that ISO induced myocardial ischemia was accomplished with a reduced fatty acids metabolism and an elevated glycolysis for energy supply upon the ischemic stress; while CDDP pretreatment prevented the tendency induced by ISO and enhanced a metabolic shift towards fatty acids metabolism that conventionally dominates energy supply to cardiac muscle cells. These data suggested that the underlying mechanism of CDDP involved regulating the dominant energy production mode and enhancing a metabolic shift toward fatty acids metabolism in ischemic heart. It was further indicated that CDDP had the potential to prevent myocardial ischemia in clinic.
authors.affiliation: Key Laboratory of Drug Metabolism and Pharmacokinetics, State Key Laboratory of Natural Medicines, Key laboratory of drug design and optimization, China Pharmaceutical University, No. 24 TongjiaLane, Nanjing, 210009, China.|State Key Laboratory of Core Technology in Innovative Chinese Medicine, Tasly R&D Institute, Tianjin Tasly Group Co., Ltd., No. 2 Pujihe East Road, Tianjin, 300410, China.
authors.forename: Jiahua
authors.initials: J
authors.lastname: Guo
authors.affiliation: Department of Cardiology, The First Affiliated Hospital of Nanjing Medical University, No. 300 Guangzhou Avenue, Nanjing, 210029, China.
authors.forename: Yonghong
authors.initials: Y
authors.lastname: Yong
authors.affiliation: Key Laboratory of Drug Metabolism and Pharmacokinetics, State Key Laboratory of Natural Medicines, Key laboratory of drug design and optimization, China Pharmaceutical University, No. 24 TongjiaLane, Nanjing, 210009, China.
authors.forename: Jiye
authors.initials: J
authors.lastname: Aa
authors.affiliation: Key Laboratory of Drug Metabolism and Pharmacokinetics, State Key Laboratory of Natural Medicines, Key laboratory of drug design and optimization, China Pharmaceutical University, No. 24 TongjiaLane, Nanjing, 210009, China.
authors.forename: Bei
authors.initials: B
authors.lastname: Cao
authors.affiliation: Key Laboratory of Drug Metabolism and Pharmacokinetics, State Key Laboratory of Natural Medicines, Key laboratory of drug design and optimization, China Pharmaceutical University, No. 24 TongjiaLane, Nanjing, 210009, China.
authors.forename: Runbin
authors.initials: R
authors.lastname: Sun
authors.affiliation: Key Laboratory of Drug Metabolism and Pharmacokinetics, State Key Laboratory of Natural Medicines, Key laboratory of drug design and optimization, China Pharmaceutical University, No. 24 TongjiaLane, Nanjing, 210009, China.
authors.forename: Xiaoyi
authors.initials: X
authors.lastname: Yu
authors.affiliation: Key Laboratory of Drug Metabolism and Pharmacokinetics, State Key Laboratory of Natural Medicines, Key laboratory of drug design and optimization, China Pharmaceutical University, No. 24 TongjiaLane, Nanjing, 210009, China.
authors.forename: Jingqiu
authors.initials: J
authors.lastname: Huang
authors.affiliation: Key Laboratory of Drug Metabolism and Pharmacokinetics, State Key Laboratory of Natural Medicines, Key laboratory of drug design and optimization, China Pharmaceutical University, No. 24 TongjiaLane, Nanjing, 210009, China.
authors.forename: Na
authors.initials: N
authors.lastname: Yang
authors.affiliation: State Key Laboratory of Core Technology in Innovative Chinese Medicine, Tasly R&D Institute, Tianjin Tasly Group Co., Ltd., No. 2 Pujihe East Road, Tianjin, 300410, China.
authors.forename: Lulu
authors.initials: L
authors.lastname: Yan
authors.affiliation: State Key Laboratory of Core Technology in Innovative Chinese Medicine, Tasly R&D Institute, Tianjin Tasly Group Co., Ltd., No. 2 Pujihe East Road, Tianjin, 300410, China.
authors.forename: Xinxin
authors.initials: X
authors.lastname: Li
authors.affiliation: State Key Laboratory of Core Technology in Innovative Chinese Medicine, Tasly R&D Institute, Tianjin Tasly Group Co., Ltd., No. 2 Pujihe East Road, Tianjin, 300410, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Cao
authors.affiliation: Department of Cardiology, The First Affiliated Hospital of Nanjing Medical University, No. 300 Guangzhou Avenue, Nanjing, 210029, China.
authors.forename: Nan
authors.initials: N
authors.lastname: Aa
authors.affiliation: Department of Cardiology, The First Affiliated Hospital of Nanjing Medical University, No. 300 Guangzhou Avenue, Nanjing, 210029, China.
authors.forename: Zhijian
authors.initials: Z
authors.lastname: Yang
authors.affiliation: Department of Cardiology, The First Affiliated Hospital of Nanjing Medical University, No. 300 Guangzhou Avenue, Nanjing, 210029, China.
authors.forename: Xiangqing
authors.initials: X
authors.lastname: Kong
authors.affiliation: Department of Cardiology, The First Affiliated Hospital of Nanjing Medical University, No. 300 Guangzhou Avenue, Nanjing, 210029, China.
authors.forename: Liansheng
authors.initials: L
authors.lastname: Wang
authors.affiliation: Key Lab of Chinese Medicine, Nanjing University of Chinese Medicine, No. 282 Hanzhong Road, Nanjing, 210029, China.
authors.forename: Xuanxuan
authors.initials: X
authors.lastname: Zhu
authors.affiliation: State Key Laboratory of Core Technology in Innovative Chinese Medicine, Tasly R&D Institute, Tianjin Tasly Group Co., Ltd., No. 2 Pujihe East Road, Tianjin, 300410, China.|School of Pharmaceutical Science and Technology, Tianjin University, No. 92 Weijin Road, Tianjin, 300072, China.
authors.forename: Xiaohui
authors.initials: X
authors.lastname: Ma
authors.affiliation: State Key Laboratory of Core Technology in Innovative Chinese Medicine, Tasly R&D Institute, Tianjin Tasly Group Co., Ltd., No. 2 Pujihe East Road, Tianjin, 300410, China.
authors.forename: Zhixin
authors.initials: Z
authors.lastname: Guo
authors.affiliation: State Key Laboratory of Core Technology in Innovative Chinese Medicine, Tasly R&D Institute, Tianjin Tasly Group Co., Ltd., No. 2 Pujihe East Road, Tianjin, 300410, China.
authors.forename: Shuiping
authors.initials: S
authors.lastname: Zhou
authors.affiliation: State Key Laboratory of Core Technology in Innovative Chinese Medicine, Tasly R&D Institute, Tianjin Tasly Group Co., Ltd., No. 2 Pujihe East Road, Tianjin, 300410, China.|School of Pharmaceutical Science and Technology, Tianjin University, No. 92 Weijin Road, Tianjin, 300072, China.
authors.forename: He
authors.initials: H
authors.lastname: Sun
authors.affiliation: Key Laboratory of Drug Metabolism and Pharmacokinetics, State Key Laboratory of Natural Medicines, Key laboratory of drug design and optimization, China Pharmaceutical University, No. 24 TongjiaLane, Nanjing, 210009, China.
authors.forename: Guangji
authors.initials: G
authors.lastname: Wang
mesh_terms: D000818:Animals; D001892:Camphanes; D004195:Disease Models, Animal; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage* / Q000494:pharmacology; D004734:Energy Metabolism / Q000187:drug effects*; D005227:Fatty Acids / Q000378:metabolism; D008401:Gas Chromatography-Mass Spectrometry; D006019:Glycolysis / Q000187:drug effects; D007545:Isoproterenol / Q000009:adverse effects*; D008297:Male; D055442:Metabolome / Q000187:drug effects; D055432:Metabolomics / Q000379:methods*; D017202:Myocardial Ischemia / Q000139:chemically induced / Q000188:drug therapy* / Q000378:metabolism; D053723:Panax notoginseng; D051381:Rats; D027561:Salvia miltiorrhiza
chemical_list: D001892:Camphanes; D004365:Drugs, Chinese Herbal; D005227:Fatty Acids; C000604051:danshen dripping pill; D007545:Isoproterenol