title: Microbaculum marinum gen. nov., sp. nov., isolated from deep seawater.
abstract: A Gram-stain-negative, aerobic, rod-shaped and non-motile bacterium, designated HSF11T, was isolated from deep seawater of the South China Sea. Growth was observed at 20-40 °C (optimum 35 °C), pH 5.0-8.5 (optimum pH 7.0-7.5) and with 0-7 % NaCl (optimum 2 %). Bacteriochlorophyll a and poly-β-hydroxybutyrate (PHB) granules were not detected. Nitrate could be reduced to nitrite. The major fatty acids (≥5 %) of strain HSF11T were C19 : 0cyclo ω8c, C18 : 0 and C16 : 0. The isoprenoid quinone was Q-10. The polar lipids were diphosphatidylglycerol, phosphatidylglycerol, two unknown phospholipids (PL1, PL2), one unknown aminolipid (AL1) and three unknown lipids (L1, L3, L4). The genomic DNA G+C content was 70.7 mol%. 16S rRNA gene sequence analysis indicated that strain HSF11T was most closely related to Tepidamorphus gemmatus DSM 19345T (95.7 % 16S rRNA gene sequence similarity) and Lutibaculum baratangense KCTC 22669T (95.2 %). On the basis of the genotypic, phenotypic, phylogenetic and chemotaxonomic characteristics, strain HSF11T represents a novel species of a new genus, for which the name Microbaculum marinum gen. nov., sp. nov. is proposed. The type strain of the type species is HSF11T (=KCTC 52 363T=MCCC 1K03192T).
authors.affiliation: Ocean College, Zhejiang University, Zhoushan 316000, PR China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Su
authors.affiliation: College of Life Sciences, Zhejiang University, Hangzhou 310058, PR China.
authors.forename: Shuaibo
authors.initials: S
authors.lastname: Han
authors.affiliation: Ocean College, Zhejiang University, Zhoushan 316000, PR China.
authors.forename: Ruijun
authors.initials: R
authors.lastname: Wang
authors.affiliation: College of Life Sciences, Zhejiang University, Hangzhou 310058, PR China.
authors.forename: Xiaoyun
authors.initials: X
authors.lastname: Yu
authors.affiliation: Ocean College, Zhejiang University, Zhoushan 316000, PR China.
authors.forename: Geyi
authors.initials: G
authors.lastname: Fu
authors.affiliation: Ocean College, Zhejiang University, Zhoushan 316000, PR China.
authors.forename: Can
authors.initials: C
authors.lastname: Chen
authors.affiliation: Ocean College, Zhejiang University, Zhoushan 316000, PR China.
authors.forename: Chongya
authors.initials: C
authors.lastname: Zhang
authors.affiliation: College of Life Sciences, Zhejiang University, Hangzhou 310058, PR China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Li
authors.affiliation: College of Life Sciences, Zhejiang Sci-Tech University, Hangzhou 310018, PR China.
authors.forename: Cong
authors.initials: C
authors.lastname: Sun
authors.affiliation: Ocean College, Zhejiang University, Zhoushan 316000, PR China.|College of Life Sciences, Zhejiang University, Hangzhou 310058, PR China.
authors.forename: Min
authors.initials: M
authors.lastname: Wu
mesh_terms: D020561:Alphaproteobacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D015373:Bacterial Typing Techniques; D001482:Base Composition; D002681:China; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D010743:Phospholipids / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012623:Seawater / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D014451:Ubiquinone / Q000031:analogs & derivatives / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D014451:Ubiquinone; C024989:coenzyme Q10