title: Analysis of Paracoccidioides lutzii mitochondria: a proteomic approach.
abstract: The genus Paracoccidioides is composed of thermal dimorphic fungi, causative agents of paracoccidioidomycosis, one of the most frequent systemic mycoses in Latin America. Mitochondria have sophisticated machinery for ATP production, which involves metabolic pathways such as citric acid and glyoxylate cycles, electron transport chain and oxidative phosphorylation. In addition, this organelle performs a variety of functions in the cell, working as an exceptional metabolic signalling centre that contributes to cellular stress responses, as autophagy and apoptosis in eukaryotic organisms. The aim of this work was to perform a descriptive proteomic analysis of mitochondria in Paracoccidioides lutzii yeast cells. After mitochondria fractionation, samples enriched in mitochondrial proteins were digested with trypsin and analysed using a NanoUPLC-MSEsystem (Waters Corporation, Manchester, UK). Ours results revealed that the established protocol for purification of mitochondria was very effective for P. lutzii, and 298 proteins were identified as primarily mitochondrial, in our analysis. To our knowledge, this is the first compilation of mitochondrial proteins from P. lutzii, to date. Copyright © 2016 John Wiley & Sons, Ltd.
authors.affiliation: Laboratório de Biologia Molecular, Instituto de Ciências Biológicas, ICBII, Campus II, Universidade Federal de Goiás, 74001-970, Goiânia, Goiás, Brazil.|Escola de Engenharia, Pontifícia Universidade Católica de Goiás, 74605-010, Goiânia, Goiás, Brazil.
authors.forename: L
authors.initials: L
authors.lastname: Casaletti
authors.affiliation: Laboratório de Biologia Molecular, Instituto de Ciências Biológicas, ICBII, Campus II, Universidade Federal de Goiás, 74001-970, Goiânia, Goiás, Brazil.
authors.forename: P S
authors.initials: PS
authors.lastname: Lima
authors.affiliation: Laboratório de Biologia Molecular, Instituto de Ciências Biológicas, ICBII, Campus II, Universidade Federal de Goiás, 74001-970, Goiânia, Goiás, Brazil.|Programa de Pós-graduação em Patologia Molecular, Faculdade de Medicina, Universidade de Brasília, 70910-900, Brasília, Distrito Federal, Brazil.
authors.forename: L N
authors.initials: LN
authors.lastname: Oliveira
authors.affiliation: Laboratório de Biologia Molecular, Instituto de Ciências Biológicas, ICBII, Campus II, Universidade Federal de Goiás, 74001-970, Goiânia, Goiás, Brazil.
authors.forename: C L
authors.initials: CL
authors.lastname: Borges
authors.affiliation: Laboratório de Microscopia, Departamento de Biologia Celular, Instituto de Ciências Biológicas, Universidade de Brasília, 70910-900, Brasília, Distrito Federal, Brazil.
authors.forename: S N
authors.initials: SN
authors.lastname: Báo
authors.affiliation: Laboratório de Biologia Molecular, Instituto de Ciências Biológicas, ICBII, Campus II, Universidade Federal de Goiás, 74001-970, Goiânia, Goiás, Brazil.
authors.forename: A M
authors.initials: AM
authors.lastname: Bailão
authors.affiliation: Laboratório de Biologia Molecular, Instituto de Ciências Biológicas, ICBII, Campus II, Universidade Federal de Goiás, 74001-970, Goiânia, Goiás, Brazil.
authors.forename: C M A
authors.initials: CM
authors.lastname: Soares
mesh_terms: D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism; D015966:Gene Expression Regulation, Fungal / Q000502:physiology; D050356:Lipid Metabolism; D008928:Mitochondria / Q000235:genetics / Q000378:metabolism*; D018384:Oxidative Stress / Q000502:physiology; D010228:Paracoccidioides / Q000235:genetics* / Q000378:metabolism*; D020543:Proteome / Q000235:genetics*; D040901:Proteomics / Q000379:methods*
chemical_list: D005656:Fungal Proteins; D020543:Proteome
keywords: MSE proteomics; Paracoccidioides lutzii; metabolism and energy production; mitochondrial proteome