title: Sensitization of immune cells following hexylaminolevulinate photodynamic therapy of cervical intraepithelial neoplasia.
abstract: BACKGROUND
  Effects of photodynamic therapy (PDT) were tested with respect to immune cell stimulation in cervical intraepithelial neoplasia (CIN).
  
  
  METHODS
  A patient with CIN received hexaminolevulinate (HAL) and subsequent PDT. These data were compared to a placebo PDT patient and a healthy HPV16-vaccinated donor. Isolation of peripheral blood mononuclear cells (PBMC) was performed before PDT and at 4 different time points after PDT. The proliferation of these PBMC was tested by [3H]thymidine incorporation following stimulation with control or HPV16-L1 peptides. Potential effects on the CD4+and CD8+cell subsets were analysed.
  
  
  RESULTS
  The data revealed an unchanged or decreased proliferation of HPV16-L1 peptide-stimulated PBMC as compared to placebo patient. HPV16-L1 peptide incubation of PBMC from the HAL patient demonstrated significant proliferative capacity after PDT. The CD4+and CD8+T cell populations in placebo patient were slightly increased after HPV16-L1 peptide administration at each time point. Mixed results were obtained for CD4+cells as compared to controls and nearly unchanged amounts of CD8+cells were detectable following HPV16-L1 peptide exposure of PBMC from the HAL/PDT-treated patient.
  
  
  CONCLUSIONS
  These findings suggest a T cell reaction from CIN patients during repeated HAL/PDT treatment. However, further immune cell populations might be involved during PDT.
authors.affiliation: Department of Obstetrics and Gynecology, Hannover Medical School, Hannover, Germany. Electronic address: Hass.Ralf@mh-hannover.de.
authors.forename: Ralf
authors.initials: R
authors.lastname: Hass
authors.affiliation: Department of Clinical Immunology and Rheumatology, Hannover Medical School, Hannover, Germany. Electronic address: Jacobs.Roland@mh-hannover.de.
authors.forename: Roland
authors.initials: R
authors.lastname: Jacobs
authors.affiliation: Clinic for Gynecology, Charité-Universitätsmedizin, Berlin, Germany. Electronic address: Andreas.Kaufmann@charite.de.
authors.forename: Andreas M
authors.initials: AM
authors.lastname: Kaufmann
authors.affiliation: Department of Obstetrics and Gynecology, Hannover Medical School, Hannover, Germany. Electronic address: Hillemanns.Peter@mh-hannover.de.
authors.forename: Peter
authors.initials: P
authors.lastname: Hillemanns
authors.affiliation: Department of Obstetrics and Gynecology, Hannover Medical School, Hannover, Germany. Electronic address: soergel.philipp@mh-hannover.de.
authors.forename: Philipp
authors.initials: P
authors.lastname: Soergel
mesh_terms: D000622:Aminolevulinic Acid / Q000031:analogs & derivatives* / Q000276:immunology / Q000494:pharmacology; D016516:CD4-CD8 Ratio; D002453:Cell Cycle; D005260:Female; D052162:Human papillomavirus 16; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000187:drug effects; D053918:Papillomavirus Vaccines / Q000276:immunology; D010778:Photochemotherapy / Q000379:methods*; D017319:Photosensitizing Agents / Q000276:immunology / Q000494:pharmacology*; D013601:T-Lymphocytes / Q000276:immunology; D002583:Uterine Cervical Neoplasms / Q000188:drug therapy; D002578:Uterine Cervical Dysplasia / Q000188:drug therapy*
chemical_list: D053918:Papillomavirus Vaccines; D017319:Photosensitizing Agents; D000622:Aminolevulinic Acid; C419924:5-aminolevulinic acid hexyl ester
keywords: Cervical intraepithelial neoplasia; Hexylaminolevulinate; Human papillomavirus; Immune cells; Photodynamic therapy