title: Glycitin regulates osteoblasts through TGF-β or AKT signaling pathways in bone marrow stem cells.
abstract: The aim of the present study was to examine the effect of glycitin on the regulation of osteoblasts from bone marrow stem cells (BMSCs) through transforming growth factor (TGF)-β or protein kinase B (AKT) signaling pathways. BMSCs were extracted from New Zealand white rabbits and used to analyze the effect of glycitin on BMSCs. BMSCs were cleared using xylene and observed via light microscopy. BMSCs were subsequently induced with glycitin (0.01, 0.5, 1, 5 and 10 µM) for 7 days, and stained with Oil Red O. The mechanism of action of glycitin on BMSCs was investigated, in which contact with collagen type I (Col I), alkaline phosphatase (ALP), TGF-β and AKT was studied. Firstly, BMSCs appeared homogeneously mazarine blue, and which showed that BMSCs were successful extracted. Administration of glycitin increased cell proliferation and promoted osteoblast formation from BMSCs. Furthermore, glycitin activated the gene expression of Col I and ALP in BMSCs. Notably, glycitin suppressed protein expression of TGF-β and AKT in BMSCs. These results indicated that glycitin may regulate osteoblasts through TGF-β or AKT signaling pathways in BMSCs.
authors.affiliation: Department of Orthopedics, General Hospital of Chinese People's Liberation Army, Beijing 100853, P.R. China; First Department of Orthopedics, Affiliated Hospital of Beihua University, Jilin 132000, P.R. China.
authors.forename: Liyan
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Department of Orthopedics, General Hospital of Chinese People's Liberation Army, Beijing 100853, P.R. China.
authors.forename: Jiying
authors.initials: J
authors.lastname: Chen
authors.affiliation: Department of Orthopedics, General Hospital of Chinese People's Liberation Army, Beijing 100853, P.R. China.
authors.forename: Wei
authors.initials: W
authors.lastname: Chai
authors.affiliation: Department of Orthopedics, General Hospital of Chinese People's Liberation Army, Beijing 100853, P.R. China.
authors.forename: Min
authors.initials: M
authors.lastname: Ni
authors.affiliation: Life Science Research Center of Beihua University, Jilin 132000, P.R. China.
authors.forename: Xin
authors.initials: X
authors.lastname: Sun
authors.affiliation: Life Science Research Center of Beihua University, Jilin 132000, P.R. China.
authors.forename: Dan
authors.initials: D
authors.lastname: Tian
keywords: bone marrow stem cells; glycitin; osteoblast; protein kinase B; transforming growth factor-β 1