title: GROWTH AND DEVELOPMENT SYMPOSIUM: Adenosine monophosphate-activated protein kinase and mitochondria in Rendement Napole pig growth.
abstract: The Rendement Napole mutation (RN-), which is well known to influence pork quality, also has a profound impact on metabolic characteristics of muscle. Pigs with RN- possess a SNP in the γ3 subunit of adenosine monophosphate (AMP)-activated protein kinase (AMPK); AMPK, a key energy sensor in skeletal muscle, modulates energy producing and energy consuming pathways to maintain cellular homeostasis. Importantly, AMPK regulates not only acute response to energy stress but also facilitates long-term adaptation via changes in gene and protein expression. The RN- allele increases AMPK activity, which alters the metabolic phenotype of skeletal muscle by increasing mitochondrial content and oxidative capacity. Fibers with greater oxidative capacity typically exhibit increased protein turnover and smaller fiber size, which indicates that RN- pigs may exhibit decreased efficiency and growth potential. However, whole body and muscle growth of RN- pigs appear similar to that of wild-type pigs and despite increased oxidative capacity, fibers maintain the capacity for hypertrophic growth. This indicates that compensatory mechanisms may allow RN- pigs to achieve rates of muscle growth similar to those of wild-type pigs. Intriguingly, lipid oxidation and mitochondria function are enhanced in RN- pig muscle. Thus far, characteristics of RN- muscle are largely based on animals near market weight. To better understand interaction between energy signaling and protein accretion in muscle, further work is needed to define age-dependent relationships between AMPK signaling, metabolism, and muscle growth.
authors.forename: T L
authors.initials: TL
authors.lastname: Scheffler
authors.forename: D E
authors.initials: DE
authors.lastname: Gerrard
mesh_terms: D055372:AMP-Activated Protein Kinases / Q000235:genetics / Q000378:metabolism*; D000483:Alleles; D000818:Animals; D004734:Energy Metabolism / Q000502:physiology; D015971:Gene Expression Regulation, Enzymologic / Q000502:physiology*; D050356:Lipid Metabolism; D008928:Mitochondria / Q000378:metabolism; D008931:Mitochondria, Muscle / Q000201:enzymology / Q000378:metabolism; D018482:Muscle, Skeletal / Q000378:metabolism; D010084:Oxidation-Reduction; D010766:Phosphorylation; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D013552:Swine / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism
chemical_list: D055372:AMP-Activated Protein Kinases