title: Uniclust databases of clustered and deeply annotated protein sequences and alignments.
abstract: We present three clustered protein sequence databases, Uniclust90, Uniclust50, Uniclust30 and three databases of multiple sequence alignments (MSAs), Uniboost10, Uniboost20 and Uniboost30, as a resource for protein sequence analysis, function prediction and sequence searches. The Uniclust databases cluster UniProtKB sequences at the level of 90%, 50% and 30% pairwise sequence identity. Uniclust90 and Uniclust50 clusters showed better consistency of functional annotation than those of UniRef90 and UniRef50, owing to an optimised clustering pipeline that runs with our MMseqs2 software for fast and sensitive protein sequence searching and clustering. Uniclust sequences are annotated with matches to Pfam, SCOP domains, and proteins in the PDB, using our HHblits homology detection tool. Due to its high sensitivity, Uniclust contains 17% more Pfam domain annotations than UniProt. Uniboost MSAs of three diversities are built by enriching the Uniclust30 MSAs with local sequence matches from MMseqs2 profile searches through Uniclust30. All databases can be downloaded from the Uniclust server at uniclust.mmseqs.com. Users can search clusters by keywords and explore their MSAs, taxonomic representation, and annotations. Uniclust is updated every two months with the new UniProt release.
authors.affiliation: Quantitative and Computational Biology Group, Max Planck Institute for Biophysical Chemistry, Göttingen, Germany.
authors.forename: Milot
authors.initials: M
authors.lastname: Mirdita
authors.affiliation: Quantitative and Computational Biology Group, Max Planck Institute for Biophysical Chemistry, Göttingen, Germany.|European Molecular Biology Laboratory, European Bioinformatics Institute (EMBL-EBI), Wellcome Trust Genome Campus, Cambridge, UK.
authors.forename: Lars
authors.initials: L
authors.lastname: von den Driesch
authors.affiliation: Quantitative and Computational Biology Group, Max Planck Institute for Biophysical Chemistry, Göttingen, Germany.
authors.forename: Clovis
authors.initials: C
authors.lastname: Galiez
authors.affiliation: European Molecular Biology Laboratory, European Bioinformatics Institute (EMBL-EBI), Wellcome Trust Genome Campus, Cambridge, UK.
authors.forename: Maria J
authors.initials: MJ
authors.lastname: Martin
authors.affiliation: Quantitative and Computational Biology Group, Max Planck Institute for Biophysical Chemistry, Göttingen, Germany soeding@mpibpc.mpg.de.
authors.forename: Johannes
authors.initials: J
authors.lastname: Söding
authors.affiliation: Quantitative and Computational Biology Group, Max Planck Institute for Biophysical Chemistry, Göttingen, Germany martin.steinegger@mpibpc.mpg.de.|Department for Bioinformatics and Computational Biology, Technische Universität München, Munich, Germany.|Department of Chemistry, Seoul National University, Seoul, Korea.
authors.forename: Martin
authors.initials: M
authors.lastname: Steinegger
mesh_terms: D016000:Cluster Analysis; D019295:Computational Biology / Q000379:methods*; D030561:Databases, Nucleic Acid; D063990:Gene Ontology; D058977:Molecular Sequence Annotation; D012984:Software; D064878:Web Browser