title: Flavobacterium luticocti sp. nov., isolated from wastewater.
abstract: A Gram-stain-negative, non-spore-forming, rod-shaped and non-motile bacterium, designated strain xz20T, was isolated from the wastewater of a municipal wastewater treatment plant in Xuzhou, China. The taxonomic status of strain xz20T was determined using a polyphasic approach. Growth occurred at 15-40 °C (optimum, 25-37 °C), at pH 6.0-8.5 (optimum, pH 7.0) and with 0-3 % (w/v) NaCl (optimum, 1-2 %). 16S rRNA gene sequence analysis revealed that strain xz20T was a member of the genus Flavobacterium and shared the highest similarity with Flavobacterium. dongtanense LW30T (95.85 %) and Flavobacterium. haoranii LQY-7T (95.20 %). The major respiratory quinone of strain xz20T was menaquinone 6 (MK-6), and the major fatty acids were iso-C15 : 0, iso-C15 : 1 G and summed feature 9 (iso-C17 : 1ω9c and/or C16 : 0 10-methyl). The major polar lipid was phosphatidylethanolamine. The genomic DNA G+C content of strain xz20T was 32.9 mol%. Based on the phenotypic, phylogenetic and chemotaxonomic characteristics, strain xz20T represents a novel species belonging to the genus Flavobacterium, for which the name Flavobacterium luticocti is proposed. The type strain is xz20T (=CCTCC AB 2015421T=JCM 31174T).
authors.affiliation: Institute of Molecular Biology and Biotechnology, Anhui Provincial Key Laboratory of the Conservation and Exploitation of Biological Resources, Anhui Normal University, No. 1 Beijing East Road, Wuhu 241000, Anhui, PR China.
authors.forename: Hongming
authors.initials: H
authors.lastname: Liu
authors.affiliation: Institute of Molecular Biology and Biotechnology, Anhui Provincial Key Laboratory of the Conservation and Exploitation of Biological Resources, Anhui Normal University, No. 1 Beijing East Road, Wuhu 241000, Anhui, PR China.
authors.forename: Peng
authors.initials: P
authors.lastname: Lu
authors.affiliation: Marine Fishery Research Institute of Zhejiang Province, Zhoushan 316021, PR China.
authors.forename: Lei
authors.initials: L
authors.lastname: Jin
authors.affiliation: Institute of Molecular Biology and Biotechnology, Anhui Provincial Key Laboratory of the Conservation and Exploitation of Biological Resources, Anhui Normal University, No. 1 Beijing East Road, Wuhu 241000, Anhui, PR China.
authors.forename: Guoping
authors.initials: G
authors.lastname: Zhu
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D002681:China; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D005417:Flavobacterium / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D010714:Phosphatidylethanolamines / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000737:chemistry; D062065:Wastewater / Q000382:microbiology*
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010714:Phosphatidylethanolamines; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; D062065:Wastewater; C483858:phosphatidylethanolamine; C042455:menaquinone 6