title: Enhancement of Neural Stem Cell Survival, Proliferation, Migration, and Differentiation in a Novel Self-Assembly Peptide Nanofibber Scaffold.
abstract: Considerable efforts have been made to combine biologically active molecules into the self-assembling peptide in order to improve cells growth, survival, and differentiation. In this study, a novel three-dimensional scaffold (RADA4GGSIKVAV; R-GSIK) was designed by adding glycine and serine between RADA4 and IKVAV to promote the strength of the peptide. The cell adhesion, viability, proliferation, migration, and differentiation of rat embryonic neural stem cells (NSCs) in R-GSIK were investigated and compared to laminin-coated, two-dimensional, and Puramatrix cultures. The scanning electron microscopy studies of the R-GSIK showed an open porous structure and a suitable surface area available for cell interaction. R-GSIK promoted the cell adhesion, viability, proliferation, and migration compared to the other cultures. In addition, the R-GSIK enhanced NSCs differentiation into neuronal cells. The NSCs injected in R-GSIK had a lower glial differentiation rate than in the Puramatrix. The results suggest that R-GSIK holds great promise for cell therapies and neuronal tissue repair.
authors.affiliation: Histology and Embryology Group, Basic Science Department, Faculty of Veterinary Medicine, Shiraz University, Shiraz, Iran.|Shefa Neuroscience Research Center, Khatam Alanbia Hospital, Tehran, Iran.|Department of Neuroscience, Mashhad University of Medical Sciences, Mashhad, Iran.
authors.forename: Sajad
authors.initials: S
authors.lastname: Sahab Negah
authors.affiliation: Histology and Embryology Group, Basic Science Department, Faculty of Veterinary Medicine, Shiraz University, Shiraz, Iran.
authors.forename: Zabihollah
authors.initials: Z
authors.lastname: Khaksar
authors.affiliation: Department of Neuroscience, School of Advanced Medical Sciences and Technologies, Shiraz University of Medical Sciences, Shiraz, Iran.
authors.forename: Hadi
authors.initials: H
authors.lastname: Aligholi
authors.affiliation: Department of Plastic and Reconstructive Surgery, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
authors.forename: Shahin
authors.initials: S
authors.lastname: Mohammad Sadeghi
authors.affiliation: Shefa Neuroscience Research Center, Khatam Alanbia Hospital, Tehran, Iran.
authors.forename: Sayed Mostafa
authors.initials: SM
authors.lastname: Modarres Mousavi
authors.affiliation: Pediatric Department, Medical Faculty, Shahed University, Tehran, Iran.
authors.forename: Hadi
authors.initials: H
authors.lastname: Kazemi
authors.affiliation: Shefa Neuroscience Research Center, Khatam Alanbia Hospital, Tehran, Iran.|Department of Clinical Biochemistry, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
authors.forename: Ali
authors.initials: A
authors.lastname: Jahanbazi Jahan-Abad
authors.affiliation: Shefa Neuroscience Research Center, Khatam Alanbia Hospital, Tehran, Iran. gorjial@uni-muenster.de.|Department of Neuroscience, Mashhad University of Medical Sciences, Mashhad, Iran. gorjial@uni-muenster.de.|Department of Neurology and Department of Neurosurgery, Westfälische Wilhelms-Universität Münster, Münster, Germany. gorjial@uni-muenster.de.|Epilepsy Research Center, Westfälische Wilhelms-Universität Münster, Münster, Germany. gorjial@uni-muenster.de.
authors.forename: Ali
authors.initials: A
authors.lastname: Gorji
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000378:metabolism; D002454:Cell Differentiation / Q000502:physiology*; D049109:Cell Proliferation / Q000502:physiology*; D002470:Cell Survival / Q000502:physiology*; D002478:Cells, Cultured; D057139:Nanofibers / Q000737:chemistry; D058953:Neural Stem Cells / Q000166:cytology*; D009474:Neurons / Q000166:cytology; D051381:Rats; D054457:Tissue Scaffolds / Q000737:chemistry
chemical_list: D001672:Biocompatible Materials
keywords: Self-assembly; Self-renewal ability; Stem cell therapy; Tissue engineering