title: Plausible exploitation of Jatropha de-oiled seed cake for lipase and phytase production and simultaneous detoxification by Candida parapsilosis isolated from poultry garbage.
abstract: Jatropha de-oiled seed cake was explored to utilize as a basic nutrient source for Candida parapsilosis, isolated from poultry garbage and selected based on the production of lipase and phytase enzymes under submerged fermentation. At optimized parameters under solid-state fermentation, lipase and phytase activities were recorded as 1056.66±2.92 and 833±2.5U/g of substrate (U/g), respectively. Besides enzyme production, complete elimination of phorbol esters and significant phytate reduction from 6.51±0.01 to 0.43±0.01g/100g of seed cake were noted after 3days incubation. Curcin and trypsin inhibition activity were reduced significantly from 26.33±0.43 to 0.56±0.02mg/100g and 229.33±2.02 to 11.66±0.28U/g, respectively after 5days incubation. Saponins were reduced from 5.56±0.19 to 1.95±0.01g/100g of seed cake after 7days incubation.
authors.affiliation: Department of Microbiology, University College of Science, Osmania University, Hyderabad, Telangana State, India.
authors.forename: Balakrishna
authors.initials: B
authors.lastname: Kannoju
authors.affiliation: Department of Microbiology, University College of Science, Osmania University, Hyderabad, Telangana State, India.
authors.forename: Swaruparani
authors.initials: S
authors.lastname: Ganapathiwar
authors.affiliation: Department of Microbiology, University College of Science, Osmania University, Hyderabad, Telangana State, India.
authors.forename: Hanumalal
authors.initials: H
authors.lastname: Nunavath
authors.affiliation: Department of Microbiology, University College of Science, Osmania University, Hyderabad, Telangana State, India.
authors.forename: Bindu
authors.initials: B
authors.lastname: Sunkar
authors.affiliation: Department of Microbiology, University College of Science, Osmania University, Hyderabad, Telangana State, India. Electronic address: bhima.ou@gmail.com.
authors.forename: Bhima
authors.initials: B
authors.lastname: Bhukya
mesh_terms: D010832:6-Phytase / Q000378:metabolism*; D000818:Animals; D002175:Candida; D005285:Fermentation; D005735:Garbage; D031286:Jatropha / Q000201:enzymology*; D008049:Lipase / Q000378:metabolism*; D010940:Plant Proteins / Q000378:metabolism*; D011200:Poultry; D012037:Refuse Disposal / Q000379:methods*; D012639:Seeds / Q000737:chemistry / Q000201:enzymology
chemical_list: D010940:Plant Proteins; D008049:Lipase; D010832:6-Phytase
keywords: Candida parapsilosis; De-oiled seed cake; Detoxification; Jatropha curcas; Lipase; Phytase; Solid-state fermentation