title: Assays to Study the Interaction of Campylobacter jejuni with the Mucosal Surface.
abstract: Mucosal colonization and overcoming the mucosal barrier are essential steps in the establishment of infection by Campylobacter jejuni. The interaction between C. jejuni and host cells, including binding and invasion, is thought to be the key virulence factor important for pathogenesis of C. jejuni infections in animals or humans. The intestinal mucosal barrier is composed of a polarized epithelium covered by a thick adherent mucus gel layer. There is a requirement for cell culture assays of infection to accurately represent the in vivo mucosal surface. In this chapter, we describe the use of a number of cell culture models and the use of polarized in vitro organ culture to examine the interaction of C. jejuni with mucosal surfaces.
authors.affiliation: Conway Institute, School of Medicine and Medical Science, University College Dublin, Dublin 12, Ireland.
authors.forename: Marguerite
authors.initials: M
authors.lastname: Clyne
authors.affiliation: Conway Institute, School of Medicine and Medical Science, University College Dublin, Dublin 12, Ireland.
authors.forename: Gina
authors.initials: G
authors.lastname: Duggan
authors.affiliation: Conway Institute, School of Medicine and Medical Science, University College Dublin, Dublin 12, Ireland.
authors.forename: Ciara
authors.initials: C
authors.lastname: Dunne
authors.affiliation: Conway Institute, School of Medicine and Medical Science, University College Dublin, Dublin 12, Ireland.
authors.forename: Brendan
authors.initials: B
authors.lastname: Dolan
authors.affiliation: Conway Institute, School of Medicine and Medical Science, University College Dublin, Dublin 12, Ireland.|National children's Research Center, Our Lady's Children's Hospital Crumlin, Dublin 12, Ireland.
authors.forename: Luis
authors.initials: L
authors.lastname: Alvarez
authors.affiliation: Conway Institute, School of Medicine and Medical Science, University College Dublin, Dublin 12, Ireland. billy.bourke@ucd.ie.
authors.forename: Billy
authors.initials: B
authors.lastname: Bourke
mesh_terms: D000818:Animals; D001422:Bacterial Adhesion / Q000187:drug effects; D001681:Biological Assay; D002169:Campylobacter Infections / Q000382:microbiology; D016123:Campylobacter jejuni / Q000254:growth & development / Q000378:metabolism* / Q000472:pathogenicity; D002645:Chickens; D003470:Culture Media / Q000737:chemistry; D017097:Electric Impedance; D005456:Fluorescent Dyes / Q000737:chemistry; D019073:HT29 Cells; D006801:Humans; D007413:Intestinal Mucosa / Q000378:metabolism* / Q000382:microbiology; D008099:Liver / Q000382:microbiology / Q000473:pathology; D008856:Microscopy, Fluorescence; D008954:Models, Biological; D009093:Mucus / Q000378:metabolism*; D009924:Organ Culture Techniques; D012235:Rhodamines / Q000737:chemistry
chemical_list: C114557:5-carboxytetramethylrhodamine succinimidyl ester; D003470:Culture Media; D005456:Fluorescent Dyes; D012235:Rhodamines
keywords: Campylobacter jejuni; HCT-8; HT-29MTX; Tissue culture; pIVOC