title: The cytoprotective effect of Rumex Aquaticus Herba extract against hydrogen peroxide-induced oxidative stress in AGS cells.
abstract: The Rumex Aquaticus Herba extract containing quercetin-3-β-D-glucuronopyranoside (ECQ) has been reported to exhibit various pharmacological activities, including anti-inflammatory and anti-oxidative effects. This plant has been traditionally used for the treatment of diarrhea, disinfestation, edema and jaundice, and as an antipyretic drug. The aim of the present study was to investigate the ability of ECQ to protect against oxidative damage and to determine its signaling mechanism in AGS cells. The protein expressions of heme oxygenase-1 (HO-1) and nuclear factor-erythroid 2 related factor 2 (Nrf2) were measured by Western blots. Cell viability was measured by MTT assay. Intracellular reactive oxygen species (ROS) levels were measured using 2',7'-dichlorofluorescein diacetate. Glutathione peroxidase levels were measured using kits. The protein expressions of HO-1 and its upstream mediator, Nrf2, increased after ECQ treatment. The HO-1 inhibitor, ZnPP, repressed the protective effect of ECQ on H2O2-induced cell damage. We found that LY294002, a specific PI3 K/Akt inhibitor, suppressed ECQ-induced HO-1 expression. ECQ significantly attenuated H2O2-induced cytotoxicity and ROS generation. Also, ECQ enhanced the antioxidant enzyme activities of glutathione peroxidase. These results suggest that ECQ exerts a cytoprotective effect against H2O2-induced oxidative stress by upregulation of Nrf2/HO-1 via the PI3 K/Akt pathway.
authors.affiliation: College of Pharmacy, Chung-Ang University, Seoul, 156-756, Republic of Korea.
authors.forename: Eun Jeong
authors.initials: EJ
authors.lastname: Cho
authors.affiliation: College of Pharmacy, Chung-Ang University, Seoul, 156-756, Republic of Korea.
authors.forename: Seung In
authors.initials: SI
authors.lastname: Um
authors.affiliation: College of Pharmacy, Chung-Ang University, Seoul, 156-756, Republic of Korea.
authors.forename: Jeong Hoon
authors.initials: JH
authors.lastname: Han
authors.affiliation: College of Pharmacy, Chung-Ang University, Seoul, 156-756, Republic of Korea.
authors.forename: Byeonghee
authors.initials: B
authors.lastname: Kim
authors.affiliation: College of Pharmacy, Chung-Ang University, Seoul, 156-756, Republic of Korea.
authors.forename: Sang Beom
authors.initials: SB
authors.lastname: Han
authors.affiliation: College of Medicine, Chung-Ang University, Seoul, 156-756, Republic of Korea.
authors.forename: Ji Hoon
authors.initials: JH
authors.lastname: Jeong
authors.affiliation: College of Medicine, Dankook University, Cheonan, Chungnam, 330-714, Republic of Korea.
authors.forename: Hak Rim
authors.initials: HR
authors.lastname: Kim
authors.affiliation: College of Medicine, Kyungpook National University, Daegu, 700-842, Republic of Korea.
authors.forename: Inkyeom
authors.initials: I
authors.lastname: Kim
authors.affiliation: College of Pharmacy, Chung-Ang University, Seoul, 156-756, Republic of Korea.
authors.forename: Wan Kyun
authors.initials: WK
authors.lastname: Whang
authors.affiliation: The Graduate School of Pharmaceutical Industry Management, Chung-Ang University, Seoul, 156-756, Republic of Korea.
authors.forename: Eunhwa
authors.initials: E
authors.lastname: Lee
authors.affiliation: College of Pharmacy, Chung-Ang University, Seoul, 156-756, Republic of Korea. udsohn@cau.ac.kr.
authors.forename: Uy Dong
authors.identifier: http://orcid.org/0000-0002-1801-7551
authors.initials: UD
authors.lastname: Sohn
mesh_terms: D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects / Q000502:physiology; D019610:Cytoprotection / Q000187:drug effects* / Q000502:physiology; D004305:Dose-Response Relationship, Drug; D006801:Humans; D006861:Hydrogen Peroxide / Q000633:toxicity*; D018384:Oxidative Stress / Q000187:drug effects* / Q000502:physiology; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology*; D017382:Reactive Oxygen Species / Q000037:antagonists & inhibitors / Q000378:metabolism; D031804:Rumex
chemical_list: D010936:Plant Extracts; D017382:Reactive Oxygen Species; D006861:Hydrogen Peroxide
keywords: Anti-inflammatory; Antioxidant; Cytoprotection; Herbal extract; Oxidative stress