title: Antimicrobial activity of different disinfectants against cariogenic microorganisms.
abstract: The aim of this study was to assess the in vitro antimicrobial effects of chlorhexidine digluconate (CHX), polyhexamethylene biguanide (PHBM), and octenidine dihydrochloride (OCT) on cariogenic microorganisms by using their minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC). CHX, PHBM, and OCT were diluted in distilled water to the final test concentrations. Using the in-tube dilution method, Streptococcus mutans, Lactobacillus acidophilus, Lactobacillus rhamnosus, and Actinomyces viscosus were cultivated on blood agar and Mueller-Hinton broth (MHB) at 37°C for 48 h. They were read using a spectrophotometer to detect MIC. To determine MBC, samples in the range of the turbidity threshold after 24 h were transferred onto blood agar and evaluated for growth after 24 h. Different MICs and MBCs were observed in all disinfectants against each microorganism. The lowest MIC and MBC against S. mutans (60 mg/L) were obtained from PHBM. The lowest values against L. rhamnosus (15 mg/L, 30 mg/L), A. viscosus (30 mg/L), and L. acidophilus (15 mg/L, 30 mg/L) were determined by OCT. PHBM and OCT have the potential to be replaced with CHX because they were effective against cariogenic microorganisms.
authors.affiliation: Izmir Katip Celebi University, Faculty of Dentistry, Department of Restorative Dentistry, Izmir, Turkey.
authors.forename: Esra
authors.initials: E
authors.lastname: Uzer Celik
authors.affiliation: Izmir Katip Celebi University, Faculty of Dentistry, Department of Restorative Dentistry, Izmir, Turkey.
authors.forename: Ayse Tugce
authors.initials: AT
authors.lastname: Tunac
authors.affiliation: Ege University, Faculty of Science, Department of Biology, Division of Basic and Industrial Microbiology, Izmir, Turkey.
authors.forename: Mustafa
authors.initials: M
authors.lastname: Ates
authors.affiliation: Private Practice, Izmir, Turkey.
authors.forename: Bilge Hakan
authors.initials: BH
authors.lastname: Sen
mesh_terms: D000362:Agar; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D000891:Anti-Infective Agents, Local / Q000494:pharmacology*; D001645:Biguanides / Q000494:pharmacology*; D002710:Chlorhexidine / Q000031:analogs & derivatives* / Q000494:pharmacology; D003731:Dental Caries / Q000382:microbiology; D006094:Gram-Positive Bacteria / Q000187:drug effects* / Q000254:growth & development; D007097:Imines; D008826:Microbial Sensitivity Tests; D011725:Pyridines / Q000494:pharmacology*; D015203:Reproducibility of Results; D013053:Spectrophotometry; D013997:Time Factors
chemical_list: D000900:Anti-Bacterial Agents; D000891:Anti-Infective Agents, Local; D001645:Biguanides; D007097:Imines; D011725:Pyridines; C031233:polihexanide; D000362:Agar; C010882:chlorhexidine gluconate; C034213:octenidine; D002710:Chlorhexidine