title: Sequential enzymatic saccharification and fermentation of ionic liquid and organosolv pretreated agave bagasse for ethanol production.
abstract: Agave bagasse (AGB) has gained recognition as a drought-tolerant biofuel feedstock with high productivity in semiarid regions. A comparative analysis of ionic liquid (IL) and organosolv (OV) pretreatment technologies in AGB was performed using a sequential enzymatic saccharification and fermentation (SESF) strategy with cellulolytic enzymes and the ethanologenic Escherichia coli strain MS04. After pretreatment, 86% of xylan and 45% of lignin were removed from OV-AGB, whereas IL-AGB reduced lignin content by 28% and xylan by 50% when compared to the untreated biomass. High glucan (>90%) and xylan (>83%) conversion was obtained with both pretreated samples. During the fermentation stage (48h), 12.1 and 12.7kg of ethanol were produced per 100kg of untreated AGB for IL and OV, respectively. These comparative analyses showed the advantages of SESF using IL and OV in a biorefinery configuration where a better understanding of AGB recalcitrance is key for future applications.
authors.affiliation: Department of Chemical Engineering, Universidad Autónoma de Nayarit, Tepic, Mexico. Electronic address: japerez@uan.edu.mx.
authors.forename: Jose A
authors.initials: JA
authors.lastname: Pérez-Pimienta
authors.affiliation: Departamento de Ingeniería Celular y Biocatálisis, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Apdo. Postal 510-3, Cuernavaca, Morelos 62250, Mexico.
authors.forename: Alejandra
authors.initials: A
authors.lastname: Vargas-Tah
authors.affiliation: Unidad Académica de Ciencias Químico Biológicos y Farmacéuticos, Universidad Autónoma de Nayarit, Tepic, Mexico.
authors.forename: Karla M
authors.initials: KM
authors.lastname: López-Ortega
authors.affiliation: Unidad Académica de Ciencias Químico Biológicos y Farmacéuticos, Universidad Autónoma de Nayarit, Tepic, Mexico.
authors.forename: Yessenia N
authors.initials: YN
authors.lastname: Medina-López
authors.affiliation: Department of Engineering in Environmental Systems, Instituto Politécnico Nacional, Mexico City, Mexico.
authors.forename: Jorge A
authors.initials: JA
authors.lastname: Mendoza-Pérez
authors.affiliation: Joint BioEnergy Institute, Biological Systems and Engineering Division, Lawrence Berkeley National Laboratory, Emeryville, CA, United States.
authors.forename: Sayeny
authors.initials: S
authors.lastname: Avila
authors.affiliation: Joint BioEnergy Institute, Biological Systems and Engineering Division, Lawrence Berkeley National Laboratory, Emeryville, CA, United States; Sandia National Laboratories, Biological and Engineering Sciences Center, Livermore, CA, United States.
authors.forename: Seema
authors.initials: S
authors.lastname: Singh
authors.affiliation: Joint BioEnergy Institute, Biological Systems and Engineering Division, Lawrence Berkeley National Laboratory, Emeryville, CA, United States; Sandia National Laboratories, Biological and Engineering Sciences Center, Livermore, CA, United States.
authors.forename: Blake A
authors.initials: BA
authors.lastname: Simmons
authors.affiliation: Departamento de Ingeniería Celular y Biocatálisis, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Apdo. Postal 510-3, Cuernavaca, Morelos 62250, Mexico.
authors.forename: Inés
authors.initials: I
authors.lastname: Loaces
authors.affiliation: Departamento de Ingeniería Celular y Biocatálisis, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Apdo. Postal 510-3, Cuernavaca, Morelos 62250, Mexico. Electronic address: alfredo@ibt.unam.mx.
authors.forename: Alfredo
authors.initials: A
authors.lastname: Martinez
mesh_terms: D027882:Agave / Q000737:chemistry / Q000378:metabolism; D056804:Biofuels; D002482:Cellulose / Q000737:chemistry / Q000378:metabolism; D000431:Ethanol / Q000032:analysis / Q000378:metabolism; D005285:Fermentation; D052578:Ionic Liquids / Q000737:chemistry*; D008031:Lignin / Q000032:analysis / Q000378:metabolism
chemical_list: D056804:Biofuels; D052578:Ionic Liquids; D000431:Ethanol; D002482:Cellulose; D008031:Lignin; C027433:bagasse
keywords: Agave bagasse; Crystallinity; Fuel ethanol; Ionic liquid; Organosolv; Sequential saccharification and fermentation