title: Identification of a hemolin protein from Actias selene mediates immune response to pathogens.
abstract: Hemolins play an important role in development and innate immunity in insects. In this study, a hemolin cDNA of 1412bp in Actias selene (As-HEM) was isolated and its open reading frames (ORFs) were 420 amino acid residues. Sequence analysis indicated As-HEM was homologous to those hemolins from other insects species. The recombinant protein of As-HEM was expressed in Escherichia coli, and anti-As-HEM antibodies were prepared. Real-time quantitative PCR (RT-qPCR) and western blot results revealed that mRNA and protein levels of As-HEM were mostly detected in hemocytes and hemolymph. Immune challenge assays showed that both the mRNA and protein levels of As-HEM could be induced significantly post Beauveria bassiana, E. coli, Micrococcus luteus and nuclear polyhedrosis virus challenges. Agglutination assays revealed that recombinant As-HEM could promote the agglutination of E. coli in the presence of calcium. Our results suggested that As-HEM was involved in the innate immunity of A. selene.
authors.affiliation: College of Life Sciences, Anhui Agricultural University, 130 Changjiang West Road, Hefei 230036, PR China.
authors.forename: Cen
authors.initials: C
authors.lastname: Qian
authors.affiliation: College of Life Sciences, Anhui Agricultural University, 130 Changjiang West Road, Hefei 230036, PR China.
authors.forename: Fang
authors.initials: F
authors.lastname: Wang
authors.affiliation: College of Life Sciences, Anhui Agricultural University, 130 Changjiang West Road, Hefei 230036, PR China.
authors.forename: Baojian
authors.initials: B
authors.lastname: Zhu
authors.affiliation: College of Life Sciences, Anhui Agricultural University, 130 Changjiang West Road, Hefei 230036, PR China.
authors.forename: Lei
authors.initials: L
authors.lastname: Wang
authors.affiliation: College of Life Sciences, Anhui Agricultural University, 130 Changjiang West Road, Hefei 230036, PR China.
authors.forename: Guoqing
authors.initials: G
authors.lastname: Wei
authors.affiliation: College of Life Sciences, Anhui Agricultural University, 130 Changjiang West Road, Hefei 230036, PR China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Sun
authors.affiliation: College of Life Sciences, Anhui Agricultural University, 130 Changjiang West Road, Hefei 230036, PR China.
authors.forename: Sheng
authors.initials: S
authors.lastname: Li
authors.affiliation: College of Life Sciences, Anhui Agricultural University, 130 Changjiang West Road, Hefei 230036, PR China. Electronic address: anhuicansang@163.com.
authors.forename: Chaoliang
authors.initials: C
authors.lastname: Liu
mesh_terms: D000372:Agglutination Tests; D000818:Animals; D052982:Beauveria / Q000276:immunology*; D003001:Cloning, Molecular; D003198:Computer Simulation; D004926:Escherichia coli / Q000276:immunology*; D006434:Hemocytes / Q000502:physiology*; D054884:Host-Pathogen Interactions; D007109:Immunity; D007136:Immunoglobulins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D019476:Insect Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D016982:Micrococcus luteus / Q000276:immunology*; D009036:Moths / Q000276:immunology* / Q000382:microbiology / Q000821:virology; D009181:Mycoses / Q000276:immunology*; D017924:Nucleopolyhedroviruses / Q000276:immunology*; D010802:Phylogeny; D017434:Protein Structure, Tertiary; D059467:Transcriptome
chemical_list: D007136:Immunoglobulins; D019476:Insect Proteins; C066846:hemolin
keywords: Actias selene; Agglutination reaction; Expression patterns; Hemolin; Immune response