title: Temporal kinetics of CD8+CD28+and CD8+CD28-T lymphocytes in the injured rat spinal cord.
abstract: This study aims to explore the temporal changes of cytotoxic CD8+CD28+and regulatory CD8+CD28-T-cell subsets in the lesion microenvironment after spinal cord injury (SCI) in rats, by combination of immunohistochemistry (IHC) and flow cytometry (FCM). In the sham-opened spinal cord, few CD8+T cells were found. After SCI, the CD8+T cells were detected at one day post-injury (dpi), then markedly increased and were significantly higher at 3, 7, and 14 dpi compared with one dpi (p < 0.01), the highest being seven dpi. In CD8+T cells, more than 90% were CD28+, and there were only small part of CD28-( < 10%). After 14 days, the infiltrated CD8+T cells were significantly decreased, and few could be found in good condition at 21 and 28 dpi. Annexin V and propidium iodide (PI) staining showed that the percentages of apoptotic/necrotic CD8+cells at 14 dpi and 21 dpi were significantly higher than those of the other early time-points (p < 0.01). These results indicate that CD8+T cells could rapidly infiltrate into the injured spinal cords and survive two weeks, however, cytotoxic CD8+T cells were dominant. Therefore, two weeks after injury might be the "time window" for treating SCI by prolonging survival times and increasing the fraction of CD8+regulatory T-cells. © 2016 Wiley Periodicals, Inc.
authors.affiliation: Clinical Laboratory, the First Affiliated Hospital of Bengbu Medical College, Anhui, 233004, P.R. China.|Anhui Key Laboratory of Tissue Transplantation, the First Affiliated Hospital of Bengbu Medical College, Anhui, 233004, P.R. China.|Department of Immunology, Bengbu Medical College, and Anhui Key Laboratory of Infection and Immunity at Bengbu Medical College, Anhui, 233030, P.R. China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Wu
authors.affiliation: Anhui Key Laboratory of Tissue Transplantation, the First Affiliated Hospital of Bengbu Medical College, Anhui, 233004, P.R. China.|Department of Immunology, Bengbu Medical College, and Anhui Key Laboratory of Infection and Immunity at Bengbu Medical College, Anhui, 233030, P.R. China.
authors.forename: Yu-Hong
authors.initials: YH
authors.lastname: Lin
authors.affiliation: Clinical Laboratory, the First Affiliated Hospital of Bengbu Medical College, Anhui, 233004, P.R. China.|Anhui Key Laboratory of Tissue Transplantation, the First Affiliated Hospital of Bengbu Medical College, Anhui, 233004, P.R. China.|Department of Immunology, Bengbu Medical College, and Anhui Key Laboratory of Infection and Immunity at Bengbu Medical College, Anhui, 233030, P.R. China.
authors.forename: Ling-Ling
authors.initials: LL
authors.lastname: Shi
authors.affiliation: Clinical Laboratory, the First Affiliated Hospital of Bengbu Medical College, Anhui, 233004, P.R. China.|Anhui Key Laboratory of Tissue Transplantation, the First Affiliated Hospital of Bengbu Medical College, Anhui, 233004, P.R. China.
authors.forename: Zong-Feng
authors.initials: ZF
authors.lastname: Yao
authors.affiliation: Clinical Laboratory, the First Affiliated Hospital of Bengbu Medical College, Anhui, 233004, P.R. China.|Anhui Key Laboratory of Tissue Transplantation, the First Affiliated Hospital of Bengbu Medical College, Anhui, 233004, P.R. China.
authors.forename: Xiu-Mei
authors.initials: XM
authors.lastname: Xie
authors.affiliation: Anhui Key Laboratory of Tissue Transplantation, the First Affiliated Hospital of Bengbu Medical College, Anhui, 233004, P.R. China.|Department of Immunology, Bengbu Medical College, and Anhui Key Laboratory of Infection and Immunity at Bengbu Medical College, Anhui, 233030, P.R. China.
authors.forename: Zheng-Song
authors.initials: ZS
authors.lastname: Jiang
authors.affiliation: Clinical Laboratory, the First Affiliated Hospital of Bengbu Medical College, Anhui, 233004, P.R. China.|Department of Immunology, Bengbu Medical College, and Anhui Key Laboratory of Infection and Immunity at Bengbu Medical College, Anhui, 233030, P.R. China.
authors.forename: Jie
authors.initials: J
authors.lastname: Tang
authors.affiliation: Clinical Laboratory, the First Affiliated Hospital of Bengbu Medical College, Anhui, 233004, P.R. China.|Anhui Key Laboratory of Tissue Transplantation, the First Affiliated Hospital of Bengbu Medical College, Anhui, 233004, P.R. China.
authors.forename: Jian-Guo
authors.initials: JG
authors.lastname: Hu
authors.affiliation: Clinical Laboratory, the First Affiliated Hospital of Bengbu Medical College, Anhui, 233004, P.R. China.|Anhui Key Laboratory of Tissue Transplantation, the First Affiliated Hospital of Bengbu Medical College, Anhui, 233004, P.R. China.|Department of Immunology, Bengbu Medical College, and Anhui Key Laboratory of Infection and Immunity at Bengbu Medical College, Anhui, 233030, P.R. China.
authors.forename: He-Zuo
authors.initials: HZ
authors.lastname: Lü
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D017304:Annexin A5 / Q000378:metabolism; D017209:Apoptosis / Q000502:physiology; D018106:CD28 Antigens / Q000378:metabolism*; D016827:CD8 Antigens / Q000378:metabolism*; D004195:Disease Models, Animal; D005260:Female; D005434:Flow Cytometry; D007700:Kinetics; D009336:Necrosis / Q000209:etiology; D051381:Rats; D017207:Rats, Sprague-Dawley; D013119:Spinal Cord Injuries / Q000150:complications / Q000276:immunology / Q000473:pathology*; D013601:T-Lymphocytes / Q000502:physiology*; D013997:Time Factors
chemical_list: D017304:Annexin A5; D018106:CD28 Antigens; D016827:CD8 Antigens
keywords: CD8+T-cell subsets; flow cytometry; microenvironment; spinal cord injury