title: Effects of previous physical training on adriamycin nephropathy and its relationship with endothelial lesions and angiogenesis in the renal cortex.
abstract: OBJECTIVE
  Adriamycin (ADR)-induced nephropathy is one of the most experimental models used in progressive kidney disease. A single dose of this drug induces a progressive and irreversible proteinuria that progresses to focal segmental glomerulosclerosis and tubulointerstitial lesions. Regular physical activity has been considered as a therapeutic intervention in several diseases. This study evaluated the influence of previous physical training in renal damage induced by ADR and the role of endothelial lesions and angiogenesis in this process.
  
  
  METHODS
  Male Wistar rats were subjected or not to treadmill running for 4weeks and then injected with ADR (2.5mg/kg, i.v.) or saline. Twenty-four-hour urine samples were collected for albuminuria measurement, and blood samples were collected to measure plasma creatinine 60days after the injections. The kidneys were removed for histological, immunohistochemical, Western blot and ELISA studies.
  
  
  RESULTS
  ADR-treated rats presented increases in plasma creatinine levels, albuminuria, podocyte damage, and enlargement of the tubular interstitial relative area, as well as higher macrophage numbers in the renal cortex, interleukin (IL)-1β levels in renal tissue and urinary monocyte chemoattractant protein (MCP)-1, which were associated with reduction in vascular endothelial growth factor (VEGF), endothelial nitric oxide synthase (eNOS) expressions and peritubular capillary (PTC) density in renal cortex. These alterations were less intense in the animals subjected to previous exercise training.
  
  
  CONCLUSIONS
  Physical training prior to ADR injection reduced the renal damage induced by this drug. This effect was related to angiogenesis and reduction in the endothelial lesions and inflammatory process in the renal cortex of these animals.
authors.affiliation: Department of Physiology, School of Medicine of Ribeirão Preto, University of São Paulo, Ribeirão Preto, São Paulo, Brazil.
authors.forename: Camila M
authors.initials: CM
authors.lastname: Faleiros
authors.affiliation: Department of Physiology, School of Medicine of Ribeirão Preto, University of São Paulo, Ribeirão Preto, São Paulo, Brazil.
authors.forename: Heloísa D C
authors.initials: HD
authors.lastname: Francescato
authors.affiliation: School of Physical Education and Sports of Ribeirão Preto, University of São Paulo, Ribeirão Preto, São Paulo, Brazil.
authors.forename: Marcelo
authors.initials: M
authors.lastname: Papoti
authors.affiliation: Department of Physiology, School of Medicine of Ribeirão Preto, University of São Paulo, Ribeirão Preto, São Paulo, Brazil.
authors.forename: Lucas
authors.initials: L
authors.lastname: Chaves
authors.affiliation: Department of Physiology, School of Medicine of Ribeirão Preto, University of São Paulo, Ribeirão Preto, São Paulo, Brazil.
authors.forename: Cleonice G A
authors.initials: CG
authors.lastname: Silva
authors.affiliation: Department of Pathology, School of Medicine of Ribeirão Preto, University of São Paulo, Ribeirão Preto, São Paulo, Brazil.
authors.forename: Roberto S
authors.initials: RS
authors.lastname: Costa
authors.affiliation: Department of Physiology, School of Medicine of Ribeirão Preto, University of São Paulo, Ribeirão Preto, São Paulo, Brazil. Electronic address: tmcoimbr@fmrp.usp.br.
authors.forename: Terezila M
authors.initials: TM
authors.lastname: Coimbra
mesh_terms: D000419:Albuminuria / Q000139:chemically induced / Q000473:pathology / Q000652:urine; D000818:Animals; D018932:Chemokine CCL2 / Q000652:urine; D003404:Creatinine / Q000097:blood; D004317:Doxorubicin; D053583:Interleukin-1beta / Q000032:analysis; D007668:Kidney / Q000098:blood supply / Q000187:drug effects / Q000473:pathology; D007672:Kidney Cortex / Q000098:blood supply* / Q000187:drug effects / Q000473:pathology*; D007674:Kidney Diseases / Q000139:chemically induced* / Q000473:pathology / Q000517:prevention & control* / Q000652:urine; D008297:Male; D052250:Nitric Oxide Synthase Type III / Q000032:analysis; D050199:Podocytes / Q000187:drug effects / Q000473:pathology; D017208:Rats, Wistar; D012420:Running; D042461:Vascular Endothelial Growth Factor A / Q000032:analysis
chemical_list: D018932:Chemokine CCL2; D053583:Interleukin-1beta; D042461:Vascular Endothelial Growth Factor A; D004317:Doxorubicin; D003404:Creatinine; D052250:Nitric Oxide Synthase Type III
keywords: Adriamycin toxicity; Angiogenesis; Endothelial lesions; Previous physical training; Renal disease progression