title: Aedes aegyptiRhesus glycoproteins contribute to ammonia excretion by larval anal papillae.
abstract: In larvalAedes aegypti, transcripts of the Rhesus-like glycoproteinsAeRh50-1andAeRh50-2have been detected in the anal papillae, sites of ammonia (NH3/NH4+) excretion; however, these putative ammonia transporters have not been previously localized or functionally characterized. In this study, we show that the AeRh50s co-immunolocalize with apical V-type H+-ATPase as well as with basal Na+/K+-ATPase in the epithelium of anal papillae. The double-stranded RNA-mediated knockdown ofAeRh50-1andAeRh50-2resulted in a significant reduction in AeRh50 protein abundance in the anal papillae, and this was coupled to decreased ammonia excretion. The knockdown ofAeRh50-1resulted in decreased hemolymph [NH4+] and pH whereas knockdown ofAeRh50-2had no effect on these parameters. We conclude that the AeRh50s are important contributors to ammonia excretion at the anal papillae of larvalA. aegypti, which may be the basis for their ability to inhabit areas with high ammonia levels.
authors.affiliation: Department of Biology, York University, 4700 Keele Street, Toronto, Ontario, Canada M3J 1P3 adurant@yorku.ca.
authors.forename: Andrea C
authors.identifier: 0000-0002-6980-4408
authors.initials: AC
authors.lastname: Durant
authors.affiliation: Department of Biology, York University, 4700 Keele Street, Toronto, Ontario, Canada M3J 1P3.
authors.forename: Helen
authors.initials: H
authors.lastname: Chasiotis
authors.affiliation: Department of Biology, York University, 4700 Keele Street, Toronto, Ontario, Canada M3J 1P3.
authors.forename: Lidiya
authors.initials: L
authors.lastname: Misyura
authors.affiliation: Department of Biology, York University, 4700 Keele Street, Toronto, Ontario, Canada M3J 1P3.
authors.forename: Andrew
authors.initials: A
authors.lastname: Donini
mesh_terms: D000330:Aedes / Q000378:metabolism*; D000641:Ammonia / Q000378:metabolism*; D000818:Animals; D006023:Glycoproteins / Q000032:analysis / Q000378:metabolism*; D006458:Hemolymph / Q000378:metabolism; D019476:Insect Proteins / Q000032:analysis / Q000378:metabolism*; D007814:Larva / Q000378:metabolism; D000254:Sodium-Potassium-Exchanging ATPase / Q000032:analysis / Q000378:metabolism; D025262:Vacuolar Proton-Translocating ATPases / Q000032:analysis / Q000378:metabolism
chemical_list: D006023:Glycoproteins; D019476:Insect Proteins; D000641:Ammonia; D025262:Vacuolar Proton-Translocating ATPases; D000254:Sodium-Potassium-Exchanging ATPase
keywords: Ammonia transporter; Mosquito; SIET; dsRNA