title: Noradrenergic inhibition of spinal hyperexcitation elicited by cutaneous cold stimuli in rats with oxaliplatin-induced allodynia: electrophysiological and behavioral assessments.
abstract: We investigated the spinal action of noradrenaline on cold-elicited hyperexcitation detected in dorsal horn neurons of rats with allodynia induced by an oxaliplatin (6 mg/kg, i.p.) injection. In vivo extracellular recordings from the spinal dorsal horn showed that wide dynamic range neurons responded to cutaneous acetone (10 μl) stimulation in normal rats, and cold-elicited firings in oxaliplatin-administered rats were increased with a longer duration, correlated with behavioral responses. These responses were significantly attenuated by spinal administration (50 μM) of noradrenaline or its agonists, clonidine (α2), phenylephrine (α1) and isoprenaline (β), in descending order of efficacy. Thus, the inhibitory effect of noradrenaline on spinal oxaliplatin-induced cold hyperexcitation is mediated mainly by activation of α2- and/or α1-adrenoceptors.
authors.affiliation: Department of Physiology, College of Korean Medicine, Kyung Hee University, Seoul, 02447, Republic of Korea.
authors.forename: Seunghwan
authors.initials: S
authors.lastname: Choi
authors.affiliation: Department of Information Physiology, National Institute for Physiological Sciences, Okazaki, 444-8787, Japan.
authors.forename: Akihiro
authors.initials: A
authors.lastname: Yamada
authors.affiliation: Department of Physiology, College of Korean Medicine, Kyung Hee University, Seoul, 02447, Republic of Korea.
authors.forename: Woojin
authors.initials: W
authors.lastname: Kim
authors.affiliation: Department of Physiology, College of Korean Medicine, Kyung Hee University, Seoul, 02447, Republic of Korea. skkim77@khu.ac.kr.
authors.forename: Sun Kwang
authors.identifier: http://orcid.org/0000-0002-2649-6652
authors.initials: SK
authors.lastname: Kim
authors.affiliation: Department of Information Physiology, National Institute for Physiological Sciences, Okazaki, 444-8787, Japan. furue@nips.ac.jp.|School of Life Science, The Graduate University for Advanced Studies (SOKENDAI), Okazaki, 444-8787, Japan. furue@nips.ac.jp.
authors.forename: Hidemasa
authors.initials: H
authors.lastname: Furue
mesh_terms: D000818:Animals; D003080:Cold Temperature; D055724:Electrophysiological Phenomena / Q000187:drug effects; D006930:Hyperalgesia / Q000139:chemically induced* / Q000188:drug therapy*; D008297:Male; D009638:Norepinephrine / Q000494:pharmacology*; D009944:Organoplatinum Compounds / Q000494:pharmacology*; D000077150:Oxaliplatin; D051381:Rats; D017207:Rats, Sprague-Dawley; D066148:Spinal Cord Dorsal Horn / Q000187:drug effects*
chemical_list: D009944:Organoplatinum Compounds; D000077150:Oxaliplatin; D009638:Norepinephrine
keywords: Cold allodynia; In vivo extracellular recording; Noradrenaline; Oxaliplatin; Spinal cord; Wide dynamic range neurons