title: PDIA3 gene induces visceral hypersensitivity in rats with irritable bowel syndrome through the dendritic cell-mediated activation of T cells.
abstract: This study investigated the mechanism of protein disulfide-isomerase A3 (PDIA3)-induced visceral hypersensitivity in irritable bowel syndrome (IBS). Rats were treated with saline (control), acetic acid and restraint stress (IBS model), empty vector (RNAi control) and PDIA3-RNAi vector (PDIA3-RNAi). Mesenteric lymph node DCs (MLNDCs) and splenic CD4+/CD8+ T cells were isolated for co-cultivation. Compared with control, MLNDCs co-cultured with CD4+ or CD8+ T cells showed an increased ability to promote T cell proliferation and produced more IL-4 or IL-9 secretion. Compared with the RNAi control, MLNDCs from the PDIA3 knockdown models were less effective in promoting the proliferation of CD4+/CD8+ T cells. It is concluded that PDIA3 plays an important role in the development of IBS through the DC-mediated activation of T cells, resulting in degranulation of MCs and visceral hypersensitivity.
authors.affiliation: Zhejiang Chinese Medical University, Hanzhou, China; Wenzhou Integrated Traditional Chinese and Western Medicine Hospital, Wenzhou Shi, Zhejiang Sheng, China.
authors.forename: Zhaomeng
authors.initials: Z
authors.lastname: Zhuang
authors.affiliation: Zhejiang Chinese Medical University , Hanzhou , China.
authors.forename: Lu
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Zhejiang Chinese Medical University , Hanzhou , China.
authors.forename: Xiaoteng
authors.initials: X
authors.lastname: Wang
authors.affiliation: Zhejiang Chinese Medical University , Hanzhou , China.
authors.forename: Liyuan
authors.initials: L
authors.lastname: Tao
authors.affiliation: Zhejiang Chinese Medical University , Hanzhou , China.
authors.forename: Bin
authors.initials: B
authors.lastname: Lv
keywords: CD4+/CD8+ T lymphocyte; Dendritic cell; IL-4; IL-9; PDIA3; Visceral hypersensitivity