title: Study of Cholesterol Repletion Effect on Nanomechanical Properties of Human Umbilical Vein Endothelial Cell Via Rapid Broadband Atomic Force Microscopy.
abstract: Abnormalities of blood cholesterol concentration are associated with increased risks for vascular disease, especially heart attacks and strokes. As one of the main lipid components of plasma membrane in all mammalian cells, cholesterol has a major impact on the mechanical properties of the membrane of endothelial cells. Although the effect of cholesterol depletion on cell mechanical properties has been studied, no results yet have been reported on quantitative investigation of cholesterol repletion effect. In this study, the cholesterol repletion effect on the nanomechanical properties of human umbilical vein endothelial cell (EA.hy926) was studied using a control-based atomic force microscope (AFM) nanomechanical measurement protocol. The viscoelasticity of EA.hy926 cells were measured over a large frequency range (0.1-100 Hz) using both constant-rate excitation force with different loading rates and a broadband excitation force. The viscoelasticity oscillation of the cell membranes under the cholesterol effect was also monitored in real-time. The experiment results showed that under the effect of cholesterol repletion, both the Young's modulus and the complex modulus of EA.hy926 cell were increased over 30%, respectively, and moreover, the amplitudes of both the elasticity oscillation and the viscosity oscillation at a period of around 200 s were increased over 70%, respectively. Therefore, this work is among the first to investigate the mechanical properties, particularly, the broadband viscoelasticity variations of EA.hy926 cells under cholesterol repletion treatment. The results revealed that cholesterol repletion may reinforce the coupling of F-actin to plasma membrane by increasing actin stability, and the cholesterol might have modified the submembrane cytoskeletal organization of EA.hy926 cell by causing the involvement of the motor protein nonmuscle myosin II.
authors.affiliation: School of Electrical Engineering, University of Electrical Science and Engineering, Chengdu 60054, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Yan
authors.affiliation: Department of Mechanical Engineering, Iowa State University, Ames, IA 50011.
authors.forename: Juan
authors.initials: J
authors.lastname: Ren
authors.affiliation: Department of Chemical Biology, Rutgers University, Piscataway, NJ 08854.
authors.forename: Yue
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Allan H. Conney Laboratory, Guangdong University of Technology, Guangzhou 510006, China.
authors.forename: Huarong
authors.initials: H
authors.lastname: Huang
authors.affiliation: Department of Chemical Biology, Rutgers University, Piscataway, NJ 08854.
authors.forename: Xi
authors.initials: X
authors.lastname: Zheng
authors.affiliation: Department of Mechanical and Aerospace Engineering, Rutgers University, Piscataway, NJ 08854 e-mail: qzzou@rci.rutgers.edu.
authors.forename: Qingze
authors.initials: Q
authors.lastname: Zou
mesh_terms: D000199:Actins / Q000378:metabolism; D001696:Biomechanical Phenomena; D002462:Cell Membrane / Q000378:metabolism; D002784:Cholesterol / Q000172:deficiency*; D055119:Elastic Modulus; D061307:Human Umbilical Vein Endothelial Cells / Q000166:cytology / Q000378:metabolism*; D006801:Humans; D055595:Mechanical Phenomena; D018625:Microscopy, Atomic Force; D036103:Nanotechnology
chemical_list: D000199:Actins; D002784:Cholesterol