title: Metabolomic Analysis of Campylobacter jejuni by Direct-Injection Electrospray Ionization Mass Spectrometry.
abstract: Direct-injection mass spectrometry (DIMS) is a means of rapidly obtaining metabolomic phenotype data in both prokaryotes and eukaryotes. Given our generally poor understanding of Campylobacter metabolism, the high-throughput and relatively simple sample preparation of DIMS has made this an attractive technique for metabolism-related studies and hypothesis generation, especially when attempting to analyze metabolic mutants with no clear phenotype. Here we describe a metabolomic fingerprinting approach with sampling and extraction methodologies optimized for direct-injection electrospray ionization mass spectrometry (ESI-MS), which we have used as a means of comparing wild-type and isogenic mutant strains of C. jejuni with various metabolic blocks.
authors.affiliation: Department of Molecular Biology and Biotechnology, The University of Sheffield, Firth Court, Western Bank, Sheffield, S10 2TN, UK.
authors.forename: Robert M
authors.initials: RM
authors.lastname: Howlett
authors.affiliation: Department of Molecular Biology and Biotechnology, The University of Sheffield, Firth Court, Western Bank, Sheffield, S10 2TN, UK.
authors.forename: Matthew P
authors.initials: MP
authors.lastname: Davey
authors.affiliation: Department of Molecular Biology and Biotechnology, The University of Sheffield, Firth Court, Western Bank, Sheffield, S10 2TN, UK. d.kelly@sheffield.ac.uk.
authors.forename: David J
authors.initials: DJ
authors.lastname: Kelly
mesh_terms: D016123:Campylobacter jejuni / Q000254:growth & development / Q000378:metabolism*; D017022:Flow Injection Analysis / Q000295:instrumentation / Q000379:methods*; D055442:Metabolome / Q000502:physiology*; D055432:Metabolomics / Q000295:instrumentation / Q000379:methods; D025341:Principal Component Analysis; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods
keywords: DIMS; Direct injection; Electrospray ionization mass spectrometry; Fingerprinting; Metabolite extraction; Metabolome; Metabolomics