title: Larvicidal activity of green synthesized silver nanoparticles usingExcoecaria agallochaL. (Euphorbiaceae) leaf extract againstAedes aegypti.
abstract: Green synthesis of silver nanoparticles (AgNPs) using plant extracts has been achieved by eco-friendly reducing and capping agents. The present study was conducted to evaluate the larvicidal efficacies of AgNPs synthesized using aqueous leaf extracts ofExcoecaria agallochaagainst dengue vector,Aedes aegypti. The 3rdand 4thinstar larvae ofA. aegyptiwere exposed to various concentrations of aqueous extracts ofE. agallocha, synthesized AgNPs and also crude solvent extracts (methanol and chloroform) for 24 h. The formation of AgNPs using aqueous leaf extracts was observed after 30 min with a characteristic colour change. The results recorded from UV-Vis spectrum, XRD, FTIR, EDX, SEM and HR-TEM were used to characterize and confirm the biosynthesis of AgNPs. The highest larvicidal efficacy of synthesized AgNPs was observed against 3rdinstar larvae at LC504.65 mg/L, LC9014.17 mg/L and 4thinstar larvae with a concentration of LC506.10 mg/L, LC9015.64 mg/L. A significant larvicidal activity was also observed with crude methanolic extracts against 3rdinstar larvae at a concentration LC5041.74 mg/L, LC90123.61 mg/L and 4thinstar larvae at a concentration of LC5052.06 mg/L, LC90166.40 mg/L as compared to the chloroform extract.
authors.affiliation: Department of Biotechnology, Acharya Nagarjuna University, Guntur 522 510, Andhra Pradesh, India.
authors.forename: Vundru
authors.initials: V
authors.lastname: Anil Kumar
authors.affiliation: Department of Botany and Microbiology, Acharya Nagarjuna University, Guntur 522 510, Andhra Pradesh, India. ammani1960@gmail.com.
authors.forename: Kandru
authors.initials: K
authors.lastname: Ammani
authors.affiliation: Centre for Microbiology, School of Life Sciences, Pondicherry University, Pondicherry 605 014, India.
authors.forename: Rajkumari
authors.initials: R
authors.lastname: Jobina
authors.affiliation: Centre for Microbiology, School of Life Sciences, Pondicherry University, Pondicherry 605 014, India.
authors.forename: Paramanandham
authors.initials: P
authors.lastname: Parasuraman
authors.affiliation: Centre for Microbiology, School of Life Sciences, Pondicherry University, Pondicherry 605 014, India.
authors.forename: Busi
authors.initials: B
authors.lastname: Siddhardha
mesh_terms: D000330:Aedes; D000818:Animals; D019665:Euphorbiaceae / Q000737:chemistry*; D055772:Green Chemistry Technology; D007306:Insecticides; D007814:Larva; D053768:Metal Nanoparticles; D000072138:Mosquito Vectors; D010936:Plant Extracts / Q000737:chemistry*; D018515:Plant Leaves / Q000737:chemistry; D012834:Silver
chemical_list: D007306:Insecticides; D010936:Plant Extracts; D012834:Silver