title: Comparative Genomics of the Conjugation Region of F-like Plasmids: Five Shades of F.
abstract: The F plasmid is the foremost representative of a large group of conjugative plasmids, prevalent inEscherichia coli, and widely distributed among the Enterobacteriaceae. These plasmids are of clinical relevance, given their frequent association with virulence determinants, colicins, and antibiotic resistance genes. Originally defined by their sensitivity to certain male-specific phages, IncF plasmids share a conserved conjugative system and regulatory circuits. In order to determine whether the genetic architecture and regulation circuits are preserved among these plasmids, we analyzed the natural diversity of F-like plasmids. Using the relaxase as a phylogenetic marker, we identified 256 plasmids belonging to the IncF/ MOBF12group, present as complete DNA sequences in the NCBI database. By comparative genomics, we identified five major groups of F-like plasmids. Each shows a particular operon structure and alternate regulatory systems. Results show that the IncF/MOBF12conjugation gene cluster conforms a diverse and ancient group, which evolved alternative regulatory schemes in its adaptation to different environments and bacterial hosts.
authors.affiliation: Instituto de Biomedicina y Biotecnologia de Cantabria Santander, Spain.
authors.forename: Raul
authors.initials: R
authors.lastname: Fernandez-Lopez
authors.affiliation: Centro de Investigacion Biomedica de la Rioja Logroño, Spain.
authors.forename: Maria
authors.initials: M
authors.lastname: de Toro
authors.affiliation: Instituto de Biomedicina y Biotecnologia de Cantabria Santander, Spain.
authors.forename: Gabriel
authors.initials: G
authors.lastname: Moncalian
authors.affiliation: Instituto de Biomedicina y Biotecnologia de Cantabria Santander, Spain.
authors.forename: M Pilar
authors.initials: MP
authors.lastname: Garcillan-Barcia
authors.affiliation: Instituto de Biomedicina y Biotecnologia de Cantabria Santander, Spain.
authors.forename: Fernando
authors.initials: F
authors.lastname: de la Cruz
keywords: IncF incompatibility group; antibiotic resistance; plasmid conjugation; plasmid evolution; plasmids