title: Enterocin B3A-B3B produced by LAB collected from infant faeces: potential utilization in the food industry for Listeria monocytogenes biofilm management.
abstract: Enterococcus faecalis B3A-B3B produces the bacteriocin B3A-B3B with activity against Listeria monocytogenes, Staphylococcus aureus, methicillin-resistant Staphylococcus aureus (MRSA) and Clostridium perfringens, but apparently not against fungi or Gram-negative bacteria, except for Salmonella Newport. B3A-B3B enterocin has two different nucleotides but similar amino acid composition to the class IIb MR10A-MR10B enterocin. B3A-B3B consists of two peptides of predicted molecular mass of 5176.31 Da (B3A) and 5182.21 Da (B3B). Importantly, B3A-B3B impeded biofilm formation of the foodborne pathogen L. monocytogenes 162 grown on stainless steel. The antimicrobial treatment of stainless steel with nisin (1 or 16 mg ml-1) decreased the cell numbers by about 2 log CFU ml-1, thereby impeding the biofilm formation by L. monocytogenes 162 or its nisin-resistant derivative strain L. monocytogenes 162R. Furthermore, the combination of nisin and B3A-B3B enterocin reduced the MIC required to inhibit this pathogen grown in planktonic or biofilm cultures.
authors.affiliation: Univ. Lille, INRA, ISA, Univ. Artois, Univ. Littoral Côte d'Opale, EA 7394-ICV (Institut Charles Viollette), 59000, Lille, France.
authors.forename: Alaa
authors.initials: A
authors.lastname: Al-Seraih
authors.affiliation: Univ. Lille, INRA, ISA, Univ. Artois, Univ. Littoral Côte d'Opale, EA 7394-ICV (Institut Charles Viollette), 59000, Lille, France. belgyan@yahoo.fr.
authors.forename: Yanath
authors.initials: Y
authors.lastname: Belguesmia
authors.affiliation: Best Environmental Technologies Inc, 9610-39 Avenue NW, Edmonton, AB, T6E 5T9, Canada.
authors.forename: John
authors.initials: J
authors.lastname: Baah
authors.affiliation: Univ. Lille, CNRS, Centrale Lille, ISEN, Univ. Valenciennes, UMR 8520 -IEMN, 59000, Lille, France.
authors.forename: Sabine
authors.initials: S
authors.lastname: Szunerits
authors.affiliation: Univ. Lille, CNRS, Centrale Lille, ISEN, Univ. Valenciennes, UMR 8520 -IEMN, 59000, Lille, France.
authors.forename: Rabah
authors.initials: R
authors.lastname: Boukherroub
authors.affiliation: Univ. Lille, INRA, ISA, Univ. Artois, Univ. Littoral Côte d'Opale, EA 7394-ICV (Institut Charles Viollette), 59000, Lille, France.
authors.forename: Djamel
authors.initials: D
authors.lastname: Drider
mesh_terms: D000900:Anti-Bacterial Agents / Q000302:isolation & purification / Q000494:pharmacology; D018441:Biofilms / Q000187:drug effects*; D001952:Bridged-Ring Compounds / Q000302:isolation & purification / Q000494:pharmacology; D013293:Enterococcus faecalis / Q000378:metabolism; D005243:Feces / Q000382:microbiology*; D005516:Food Microbiology; D006801:Humans; D008089:Listeria monocytogenes / Q000187:drug effects*; D008826:Microbial Sensitivity Tests; D009561:Nisin / Q000494:pharmacology
chemical_list: D000900:Anti-Bacterial Agents; D001952:Bridged-Ring Compounds; D009561:Nisin; C012306:enterocin; C102526:nisin A
keywords: Biofilm; Class IIb Enterocin B3A-B3B; Listeria monocytogenes; Nisin; Resistance