title: Lab-on-a-Chip Multiplex Assays.
abstract: Lab-on-a-chip multiplex assays allow a rapid identification of multiple parameters in an automated manner. Here we describe a lab-based preparation followed by a rapid and fully automated DNA microarray hybridization and readout in less than 10 min using the Fraunhofer in vitro diagnostics (ivD) platform to enable rapid identification of bacterial species and detection of antibiotic resistance. The use of DNA microarrays allows a fast adaptation of new biomarkers enabling the identification of different genes as well as single-nucleotide-polymorphisms (SNPs) within these genes. In this protocol we describe a DNA microarray developed for identification of Staphylococcus aureus and the mecA resistance gene.
authors.affiliation: Fraunhofer Institute for Cell Therapy and Immunology, Branch Bioanalytics and Bioprocesses (IZI-BB), Am Mühlenberg 13, 14476, Potsdam, Germany. Harald.Peter@izi-bb.fraunhofer.de.
authors.forename: Harald
authors.initials: H
authors.lastname: Peter
authors.affiliation: Fraunhofer Institute for Cell Therapy and Immunology, Branch Bioanalytics and Bioprocesses (IZI-BB), Am Mühlenberg 13, 14476, Potsdam, Germany.
authors.forename: Julia
authors.initials: J
authors.lastname: Wienke
authors.affiliation: Fraunhofer Institute for Cell Therapy and Immunology, Branch Bioanalytics and Bioprocesses (IZI-BB), Am Mühlenberg 13, 14476, Potsdam, Germany.
authors.forename: Frank F
authors.initials: FF
authors.lastname: Bier
mesh_terms: D057205:Automation, Laboratory; D024881:Drug Resistance, Bacterial; D060005:Genotyping Techniques; D006801:Humans; D056656:Lab-On-A-Chip Devices; D025202:Molecular Diagnostic Techniques; D020411:Oligonucleotide Array Sequence Analysis / Q000295:instrumentation / Q000379:methods*; D019095:Point-of-Care Systems; D020641:Polymorphism, Single Nucleotide; D012984:Software; D013203:Staphylococcal Infections / Q000175:diagnosis / Q000382:microbiology; D013211:Staphylococcus aureus / Q000187:drug effects / Q000235:genetics; D057188:Workflow
keywords: Antibiotic resistance detection; Antimicrobial drug resistance; Automated DNA microarray hybridization; Bacterial infection; Early diagnosis; Genotyping; Lab-on-a-chip; MRSA detection; Point-of-care; SNP detection