title: Large-Scale Interaction Profiling of Protein Domains Through Proteomic Peptide-Phage Display Using Custom Peptidomes.
abstract: Protein-protein interactions are essential to cellular functions and signaling pathways. We recently combined bioinformatics and custom oligonucleotide arrays to construct custom-made peptide-phage libraries for screening peptide-protein interactions, an approach we call proteomic peptide-phage display (ProP-PD). In this chapter, we describe protocols for phage display for the identification of natural peptide binders for a given protein. We finally describe deep sequencing for the analysis of the proteomic peptide-phage display.
authors.affiliation: Terrence Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, Toronto, ON, Canada, M5S 3E1.
authors.forename: Moon-Hyeong
authors.initials: MH
authors.lastname: Seo
authors.affiliation: Terrence Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, Toronto, ON, Canada, M5S 3E1.
authors.forename: Satra
authors.initials: S
authors.lastname: Nim
authors.affiliation: Terrence Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, Toronto, ON, Canada, M5S 3E1.
authors.forename: Jouhyun
authors.initials: J
authors.lastname: Jeon
authors.affiliation: Terrence Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, Toronto, ON, Canada, M5S 3E1. pi@kimlab.org.|Department of Molecular Genetics, University of Toronto, 160 College Street, Room 230, Toronto, ON, Canada, M5S 3E1. pi@kimlab.org.|Department of Computer Science, University of Toronto, 160 College Street, Room 230, Toronto, ON, Canada, M5S 3E1. pi@kimlab.org.
authors.forename: Philip M
authors.initials: PM
authors.lastname: Kim
mesh_terms: D004797:Enzyme-Linked Immunosorbent Assay; D059014:High-Throughput Nucleotide Sequencing; D055767:Immobilized Proteins / Q000302:isolation & purification / Q000378:metabolism; D046228:Microarray Analysis; D009841:Oligonucleotides / Q000378:metabolism; D019151:Peptide Library; D010455:Peptides / Q000737:chemistry* / Q000378:metabolism*; D000072417:Protein Domains; D025941:Protein Interaction Mapping / Q000379:methods*; D020543:Proteome / Q000378:metabolism*; D040901:Proteomics / Q000379:methods*
chemical_list: D055767:Immobilized Proteins; D009841:Oligonucleotides; D019151:Peptide Library; D010455:Peptides; D020543:Proteome
keywords: DNA synthesis; Next-generation sequencing; Oligo microarray; Phage display; Protein–protein interaction