title: Expression and biochemical characteristics of two different aldosterone receptors in both healthy and dilated cardiomyopathy dog heart tissue.
abstract: Recently, replicates of the aldosterone receptor expression have been done in healthy heart dog tissues through immunohistochemistry, showing an apparent heterogeneous distribution in the four chambers. Recent studies have also identified immediate effects of aldosterone, suggesting aldosterone also produces non-genomic effects caused by an unidentified receptor. In order to study the molecular and quantitative expression characteristics of aldosterone binding receptors in the canine heart, we conducted studies, using Western Blot, in the heart from both healthy animals and animals with dilated cardiomyopathy. The results show the presence and distribution of two aldosterone receptors; one of 110/120 kDa molecular weight, suggested as cytosolic/nuclear and the other of undetermined location with a 250 kDa molecular weight.
authors.affiliation: Research Laboratory in Physiology and Pharmacology, Faculty of Veterinary Medicine, Autonomous University of Puebla, 4 Sur #304, Centro, Puebla, Mexico. arepal@hotmail.com.
authors.forename: Alejandro R
authors.initials: AR
authors.lastname: Reynoso Palomar
authors.affiliation: Research Laboratory in Physiology and Pharmacology, Faculty of Veterinary Medicine, Autonomous University of Puebla, 4 Sur #304, Centro, Puebla, Mexico.
authors.forename: Moncerrat
authors.initials: M
authors.lastname: Rodriguez Bravo
authors.affiliation: Research Laboratory in Physiology and Pharmacology, Faculty of Veterinary Medicine, Autonomous University of Puebla, 4 Sur #304, Centro, Puebla, Mexico.
authors.forename: Abel E
authors.initials: AE
authors.lastname: Villa Mancera
authors.affiliation: Faculty of Veterinary Sciences, National University of Central Buenos Aires Province, Buenos Aires, Argentina.
authors.forename: Carlos J
authors.initials: CJ
authors.lastname: Mucha
mesh_terms: D000818:Animals; D002311:Cardiomyopathy, Dilated / Q000235:genetics / Q000503:physiopathology / Q000662:veterinary*; D004283:Dog Diseases / Q000503:physiopathology*; D004285:Dogs; D005786:Gene Expression Regulation; D021381:Protein Transport; D018161:Receptors, Mineralocorticoid / Q000235:genetics* / Q000378:metabolism*
chemical_list: D018161:Receptors, Mineralocorticoid
keywords: Aldosterone receptor; Cardiomyopathy; Dog heart; Mineralocorticoid receptor