title: Characterization of Salinity Tolerance of Transgenic Rice Lines HarboringHsCBL8of Wild Barley (Hordeum spontanum) Line from Qinghai-Tibet Plateau.
abstract: Rice is more sensitive to salinity, particularly at its early vegetative and later productive stages. Wild plants growing in harsh environments such as wild barley from Qinghai-Tibet Plateau adapt to the adverse environment with allelic variations at the loci responsible for stressful environment, which could be used for rice genetic improvement. In this study, we overexpressedHsCBL8encoding a calcium-sensor calcineurin B-like (CBL) protein in rice. The gene was isolated from XZ166, a wild-barley (Hordeum spontanum) line originated from Qinghai-Tibet Plateau. We found that XZ166 responded to high NaCl concentration (200 mM) with moreHsCBL8transcripts than CM72, a cultivated barley line known for salinity tolerance. XZ166 is significantly different from CM72 with nucleotide sequences atHsCBL8. The overexpression ofHsCBL8in rice resulted in significant improvement of water protectionin vivoand plasma membrane, more proline accumulation, and a reduction of overall Na+uptake but little change in K+concentration in the plant tissues. Notably,HsCBL8did not act on some genes downstream of the rice CBL family genes, suggesting an interesting interaction betweenHsCBL8and unknown factors to be further investigated.
authors.affiliation: Institute of Crop Science, College of Agriculture and Biotechnology, Zhejiang UniversityHangzhou, China; Department of Biotechnology, College of Life Science, Zhejiang Sci-Tech UniversityHangzhou, China.
authors.forename: Wanli
authors.initials: W
authors.lastname: Guo
authors.affiliation: Institute of Crop Science, College of Agriculture and Biotechnology, Zhejiang University Hangzhou, China.
authors.forename: Tianlong
authors.initials: T
authors.lastname: Chen
authors.affiliation: Institute of Crop Science, College of Agriculture and Biotechnology, Zhejiang University Hangzhou, China.
authors.forename: Nazim
authors.initials: N
authors.lastname: Hussain
authors.affiliation: Institute of Crop Science, College of Agriculture and Biotechnology, Zhejiang University Hangzhou, China.
authors.forename: Guoping
authors.initials: G
authors.lastname: Zhang
authors.affiliation: Institute of Crop Science, College of Agriculture and Biotechnology, Zhejiang University Hangzhou, China.
authors.forename: Lixi
authors.initials: L
authors.lastname: Jiang
keywords: Arabidopsis; Hordeum spontanum; HsCBL8; rice; salt tolerance