title: Flavobacterium inkyongense sp. nov., isolated from an artificial freshwater pond.
abstract: A Gram-stain-negative, non-motile, yellow-pigmented bacterium, designated strain IMCC27201T, was isolated from an artificial freshwater pond (Inkyong) in Korea. Growth of strain IMCC27201T occurred at 10-37 °C (optimum, 30 °C), at pH 7.0-8.0 (optimum, pH 7.0) and with 0-0.25 % (w/v) NaCl (optimum, 0 %). Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain IMCC27201T belonged to the genus Flavobacterium and was related most closely to Flavobacterium columnare IFO 15943T (97.8 % sequence similarity), Flavobacterium terrae R2A1-13T (97.2 %) and Flavobacterium vireti THG-SM1T (96.4 %). Strain IMCC27201T exhibited low DNA-DNA relatedness with F. columnare KCTC 42885T (34.9-40.6 %). The DNA G+C content of strain IMCC27201T was 32.1 mol%. The major fatty acids were iso-C15 : 0, iso-C15 : 1 G and iso-C15 : 0 3-OH. Strain IMCC27201T contained menaquinone-6 (MK-6) as the sole isoprenoid quinone. The polar lipids of strain IMCC27201T consisted of phosphatidylethanolamine, three unidentified aminolipids and two unidentified lipids. Based on 16S rRNA gene phylogeny, phenotypic characterization and DNA-DNA relatedness, strain IMCC27201Trepresents a novel species of the genus Flavobacterium, for which the name Flavobacterium inkyongense sp. nov. is proposed. The type strain is IMCC27201T (=KCTC 52244T=JCM 31385T).
authors.affiliation: Department of Biological Sciences, Inha University, Incheon 22212, Republic of Korea.
authors.forename: Miri
authors.initials: M
authors.lastname: Park
authors.affiliation: Department of Biological Sciences, Inha University, Incheon 22212, Republic of Korea.
authors.forename: Yochan
authors.initials: Y
authors.lastname: Joung
authors.affiliation: Department of Biological Sciences, Inha University, Incheon 22212, Republic of Korea.
authors.forename: Gi Gyun
authors.initials: GG
authors.lastname: Nam
authors.affiliation: Department of Biological Sciences, Inha University, Incheon 22212, Republic of Korea.
authors.forename: Suhyun
authors.initials: S
authors.lastname: Kim
authors.affiliation: Department of Biological Sciences, Inha University, Incheon 22212, Republic of Korea.
authors.forename: Jang-Cheon
authors.initials: JC
authors.lastname: Cho
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D005417:Flavobacterium / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D005618:Fresh Water / Q000382:microbiology; D009693:Nucleic Acid Hybridization; D010714:Phosphatidylethanolamines / Q000737:chemistry; D010802:Phylogeny; D061005:Ponds / Q000382:microbiology*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D056910:Republic of Korea; D017422:Sequence Analysis, DNA; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010714:Phosphatidylethanolamines; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; C483858:phosphatidylethanolamine; C042455:menaquinone 6