title: Methyl-CpG-binding domain 3 inhibits epithelial-mesenchymal transition in pancreatic cancer cells via TGF-β/Smad signalling.
abstract: Methyl-CpG-binding domain 3 (MBD3) is an aberrant expression in human malignancies. However, the role of MBD3 in pancreatic cancer progression remains to be clarified. In this study, we investigated the effects of MBD3 on the epithelial-mesenchymal transition (EMT), and the underlying mechanism in pancreatic cancer cells.
  
  
  
  The abilities of migration and invasion were examined by transwell and BD Matrigel invasion assays. EMT and TGF-β/Smad signalling were evaluated.
  
  
  
  First, we find that MBD3 expression is lower in pancreatic cancer tissues than that in non-tumour tissues, and patients with lower MBD3 levels survive significantly less than those with higher levels. Subsequently, we find that MBD3 knockdown promotes the abilities of migration and invasion, while MBD3 overexpression inhibits the above abilities. Also, MBD3 knockdown remarkably increases mesenchymal markers expression of Vimentin, α-SMA, Snail, N-cadherin, β-catenin, and downregulates epithelial markers expression of E-cadherin. On the contrary, MBD3 overexpression results in the opposite effects. Further evidence reveals that MBD3 knockdown up-regulates expression of TGF-β, and then activates p-Smad2 and p-Smad3, while MBD3 overexpression results in downregulation of TGF-β, p-Smad2, and p-Smad3.
  
  
  
  MBD3 inhibits EMT in pancreatic cancer cells probably via TGF-β/Smad signalling, and may be a new candidate target for diagnostics and prognosis of pancreatic cancer.
authors.affiliation: Department of Gastroenterology, Affiliated Hospital of Jiangsu University, Jiangsu University, Zhenjiang, China.
authors.forename: Min
authors.initials: M
authors.lastname: Xu
authors.affiliation: Department of Gastroenterology, Affiliated Hospital of Jiangsu University, Jiangsu University, Zhenjiang, China.
authors.forename: Junbo
authors.initials: J
authors.lastname: He
authors.affiliation: Department of Gastroenterology, Affiliated Hospital of Jiangsu University, Jiangsu University, Zhenjiang, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Li
authors.affiliation: Department of Gastroenterology, Affiliated Hospital of Jiangsu University, Jiangsu University, Zhenjiang, China.
authors.forename: Wen
authors.initials: W
authors.lastname: Feng
authors.affiliation: Department of Gastroenterology, Affiliated Hospital of Jiangsu University, Jiangsu University, Zhenjiang, China.
authors.forename: Hailang
authors.initials: H
authors.lastname: Zhou
authors.affiliation: Department of Gastroenterology, Affiliated Hospital of Jiangsu University, Jiangsu University, Zhenjiang, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Wei
authors.affiliation: Department of Gastroenterology, Affiliated Hospital of Jiangsu University, Jiangsu University, Zhenjiang, China.
authors.forename: Meng
authors.initials: M
authors.lastname: Zhou
authors.affiliation: Department of Gastroenterology, Affiliated Hospital of Jiangsu University, Jiangsu University, Zhenjiang, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Lu
authors.affiliation: Department of Cell Biology, School of Medicine, Jiangsu University, Zhenjiang, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Zeng
authors.affiliation: Department of Cell Biology, School of Medicine, Jiangsu University, Zhenjiang, China.
authors.forename: Wanxin
authors.initials: W
authors.lastname: Peng
authors.affiliation: Department of Cell Biology, School of Medicine, Jiangsu University, Zhenjiang, China.
authors.forename: Fengyi
authors.initials: F
authors.lastname: Du
authors.affiliation: Department of Cell Biology, School of Medicine, Jiangsu University, Zhenjiang, China.
authors.forename: Aihua
authors.initials: A
authors.lastname: Gong
mesh_terms: D000230:Adenocarcinoma / Q000175:diagnosis / Q000235:genetics / Q000473:pathology*; D014408:Biomarkers, Tumor / Q000235:genetics; D045744:Cell Line, Tumor; D004268:DNA-Binding Proteins / Q000502:physiology*; D058750:Epithelial-Mesenchymal Transition / Q000235:genetics*; D057809:HEK293 Cells; D006801:Humans; D010190:Pancreatic Neoplasms / Q000175:diagnosis / Q000235:genetics / Q000473:pathology*; D011379:Prognosis; D015398:Signal Transduction / Q000235:genetics; D051785:Smad Proteins / Q000378:metabolism*; D016212:Transforming Growth Factor beta / Q000378:metabolism*
chemical_list: D014408:Biomarkers, Tumor; D004268:DNA-Binding Proteins; C115369:MBD3 protein, human; D051785:Smad Proteins; D016212:Transforming Growth Factor beta