title: Methylation of promoter region ofCDX2gene in colorectal cancer.
abstract: The incidence of colorectal cancer is on the increase owing to changes in daily diet. In the present study, the methylation status of caudal type homeobox transcription factor 2 (CDX2) gene in lesion tissue of colorectal cancer (CRC) was investigated. Additionally, the correlation between the promoter methylation ofCDX2gene, CRC and gene expression in patients with CRC and normal population was examined. Between April 2014 and May 2015 78 cases with CRC were enrolled in the study. Using methylation-specific polymerase chain reaction (PCR), the promoter methylation of CDX2 in normal tissues and colorectal tissues was examinned. Through the fluorescence quantitative PCR technique, the expression levels ofCDX2gene were determined in a normal population and lesion tissue of patients with CRC. At the same time, we evaluated the levels of theCDX2gene product in the normal population and lesion tissue of patients with CRC. The results showed that the methylation rate of the promoter region ofCDX2gene in normal colorectal tissue was 43.5%, whereas that in the lesion tissue of CRC was 78.5%. The result was statistically significant (P<0.05). The quantity of mRNA and protein expression ofCDX2gene in colorectal and normal tissue was significantly different (P<0.05). In conclusion, the methylation of theCDX2gene promoter region was associated with risk of CRC, i.e., methylation of the promoter region ofCDX2gene favors the occurrence of CRC.
authors.affiliation: Luoyang Central Hospital Affiliated to Zhengzhou University, Luoyang, Henan 471000, P.R. China.
authors.forename: Yunshuai
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Luoyang Central Hospital Affiliated to Zhengzhou University, Luoyang, Henan 471000, P.R. China.
authors.forename: Zhaohui
authors.initials: Z
authors.lastname: Li
authors.affiliation: Luoyang Central Hospital Affiliated to Zhengzhou University, Luoyang, Henan 471000, P.R. China.
authors.forename: Wenxian
authors.initials: W
authors.lastname: Li
authors.affiliation: Luoyang Central Hospital Affiliated to Zhengzhou University, Luoyang, Henan 471000, P.R. China.
authors.forename: Shuaifeng
authors.initials: S
authors.lastname: Liu
authors.affiliation: Luoyang Central Hospital Affiliated to Zhengzhou University, Luoyang, Henan 471000, P.R. China.
authors.forename: Baowei
authors.initials: B
authors.lastname: Han
keywords: CDX2 gene; colorectal; gene expression; methylation; promoter