title: Involvement of cAMP receptor protein in biofilm formation, fimbria production, capsular polysaccharide biosynthesis and lethality in mouse of Klebsiella pneumoniae serotype K1 causing pyogenic liver abscess.
abstract: The global regulator cAMP receptor protein (CRP) has been shown to be required for the full virulence and/or for the expression of virulence determinants in a wide set of bacterial pathogens. In this work, the crp mutant as well as the complemented mutant was constructed from a wild-type Klebsiella pneumoniae capsular serotype K1 strain causing the primary pyogenic liver abscess. The phenotypes of wild-type strain, crp mutant and complemented mutant were characterized systematically. It was disclosed that K. pneumoniae CRP was required for the in vitro growth, fimbria production, biofilm formation and lethality in mouse, but it inhibited the capsular polysaccharide biosynthesis. These indicated the important roles of CRP in regulating the expression of virulence and biofilm genes in K. pneumoniae.
authors.affiliation: School of Basic Medicine, Hubei University of Medicine, Shiyan, Hubei 442000, PR China.
authors.forename: Qin
authors.initials: Q
authors.lastname: Ou
authors.affiliation: School of Basic Medicine, Hubei University of Medicine, Shiyan, Hubei 442000, PR China.
authors.forename: Jinming
authors.initials: J
authors.lastname: Fan
authors.affiliation: Department of Dermatology, Taihe Hospital, Hubei University of Medicine, Shiyan, Hubei 442000, PR China.
authors.forename: Dejian
authors.initials: D
authors.lastname: Duan
authors.affiliation: School of Basic Medicine, Hubei University of Medicine, Shiyan, Hubei 442000, PR China.
authors.forename: Li
authors.initials: L
authors.lastname: Xu
authors.affiliation: State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, PR China.
authors.forename: Jie
authors.initials: J
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, PR China.
authors.forename: Dongsheng
authors.initials: D
authors.lastname: Zhou
authors.affiliation: State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, PR China.
authors.forename: Huiying
authors.initials: H
authors.lastname: Yang
authors.affiliation: Department of Dermatology, Taihe Hospital, Hubei University of Medicine, Shiyan, Hubei 442000, PR China.|School of Basic Medicine, Hubei University of Medicine, Shiyan, Hubei 442000, PR China.
authors.forename: Bei
authors.initials: B
authors.lastname: Li
mesh_terms: D000818:Animals; D016667:Bacterial Capsules / Q000378:metabolism; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D018441:Biofilms / Q000254:growth & development; D002373:Cyclic AMP Receptor Protein / Q000235:genetics / Q000378:metabolism*; D005260:Female; D010861:Fimbriae, Bacterial / Q000378:metabolism*; D017353:Gene Deletion; D015964:Gene Expression Regulation, Bacterial; D007711:Klebsiella pneumoniae / Q000235:genetics / Q000378:metabolism / Q000472:pathogenicity*; D046290:Liver Abscess, Pyogenic / Q000382:microbiology* / Q000473:pathology; D051379:Mice; D008807:Mice, Inbred BALB C; D011135:Polysaccharides, Bacterial; D012329:RNA, Bacterial / Q000235:genetics; D012703:Serotyping; D014774:Virulence
chemical_list: D001426:Bacterial Proteins; D002373:Cyclic AMP Receptor Protein; D011135:Polysaccharides, Bacterial; D012329:RNA, Bacterial