title: Toll-like receptor 2 is increased in neurons in Parkinson's disease brain and may contribute to alpha-synuclein pathology.
abstract: Inflammation is likely a key contributor to the pathogenesis of Parkinson's disease (PD), a progressively debilitating neurodegenerative disease that is accompanied by a pathological accumulation of the α-synuclein protein in a staged manner through the brain. What leads to the accumulation of α-synuclein in PD and how this relates to inflammatory pathways, however, is not entirely clear. Toll-like receptor (TLR) signaling is a major pathway mediating inflammation and, in particular, TLR2 is increasingly being implicated in PD. We have, therefore, examined the expression of TLR2 in postmortem brain tissue from PD patients and matched controls. We confirm that TLR2 is increased in PD brain, and find that levels of TLR2 correlate with the accumulation of pathological α-synuclein. TLR2 was expressed on neurons as well as microglia; however, the neuronal rather than glial expression of TLR2 was significantly increased in PD brain in accordance with disease staging, and TLR2 was strongly localized to α-synuclein positive Lewy bodies. In cell culture, activation of neuronal TLR2 induced an inflammatory response, including the secretion of inflammatory cytokines and microglial-activating chemokines, as well as the production of reactive oxygen species. Moreover, activation of neuronal TLR2 increased levels of endogenous α-synuclein protein, which was in turn associated with increased levels of the autophagy/lysosomal pathway marker p62. Finally, promoting autophagy with rapamycin or pharmacological inhibition of the TLR2 signaling pathway prevented the TLR2-mediated increase in α-synuclein in neuronal cell cultures. These results implicate neuronal TLR2 expression in human PD pathogenesis. In particular, the increased expression of TLR2 on neurons may provide new insight into disease pathogenesis and/or options for therapeutic intervention.
authors.affiliation: Neuroscience Research Australia, Barker Street, Randwick, NSW, 2031, Australia. n.dzamko@neura.edu.au.|School of Medical Sciences, University of NSW, Sydney, NSW, 2052, Australia. n.dzamko@neura.edu.au.
authors.forename: Nicolas
authors.initials: N
authors.lastname: Dzamko
authors.affiliation: Neuroscience Research Australia, Barker Street, Randwick, NSW, 2031, Australia.
authors.forename: Amanda
authors.initials: A
authors.lastname: Gysbers
authors.affiliation: Neuroscience Research Australia, Barker Street, Randwick, NSW, 2031, Australia.
authors.forename: Gayathri
authors.initials: G
authors.lastname: Perera
authors.affiliation: Neuroscience Research Australia, Barker Street, Randwick, NSW, 2031, Australia.
authors.forename: Anita
authors.initials: A
authors.lastname: Bahar
authors.affiliation: Neuroscience Research Australia, Barker Street, Randwick, NSW, 2031, Australia.|School of Medical Sciences, University of NSW, Sydney, NSW, 2052, Australia.
authors.forename: Amrita
authors.initials: A
authors.lastname: Shankar
authors.affiliation: Neuroscience Research Australia, Barker Street, Randwick, NSW, 2031, Australia.|School of Medical Sciences, University of NSW, Sydney, NSW, 2052, Australia.
authors.forename: Jianqun
authors.initials: J
authors.lastname: Gao
authors.affiliation: Neuroscience Research Australia, Barker Street, Randwick, NSW, 2031, Australia.|School of Medical Sciences, University of NSW, Sydney, NSW, 2052, Australia.
authors.forename: YuHong
authors.initials: Y
authors.lastname: Fu
authors.affiliation: Neuroscience Research Australia, Barker Street, Randwick, NSW, 2031, Australia. g.halliday@neura.edu.au.|School of Medical Sciences, University of NSW, Sydney, NSW, 2052, Australia. g.halliday@neura.edu.au.
authors.forename: Glenda M
authors.initials: GM
authors.lastname: Halliday
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D001921:Brain / Q000276:immunology* / Q000378:metabolism / Q000473:pathology*; D005260:Female; D006801:Humans; D007249:Inflammation / Q000276:immunology / Q000473:pathology; D008297:Male; D008875:Middle Aged; D010300:Parkinson Disease / Q000276:immunology* / Q000378:metabolism / Q000473:pathology*; D051195:Toll-Like Receptor 2 / Q000378:metabolism*; D051844:alpha-Synuclein / Q000378:metabolism
chemical_list: C495342:TLR2 protein, human; D051195:Toll-Like Receptor 2; D051844:alpha-Synuclein
keywords: Autophagy; Inflammation; Parkinson’s disease; Toll-like receptor; α-Synuclein