title: [Effects of different fluid resuscitation program on renal function in swine during shock stage of severe burn].
abstract: Objective:To explore the effects of different fluid resuscitation program on renal function in swine during shock stage of severe burn.Methods:Twenty-four Guangxi Bama miniature swine were inflicted with 40% total body surface area on the back, and then they were divided into four groups according to the random number table, with 6 swine in each group. At post injury hour (PIH) 2, swine in succinylated gelatin group (S), hydroxyethyl starch group (H), and allogeneic plasma group (A) were respectively resuscitated with succinylated gelatin, hydroxyethyl starch 130/0.4, and plasma according to burn shock " domestic general" resuscitation formula, and swine in Parkland group (P) were resuscitated with lactated Ringer's solution according to Parkland formula. Hemodynamic indexes including heart rate, blood pressure, urine volume, pulmonary capillary wedge pressure, and central venous pressure before injury, at the first and second PIH 24 were recorded. The volume of resuscitation fluid was calculated at the first and second PIH 24. Blood and urine samples were collected before injury and at PIH 4, 8, 24, and 48, and then serum creatinine and urea nitrogen were detected by automatic biochemical analyzer, urine microalbumin and urine creatinine were detected by automated urine analyzer and the ratio of which was calculated. The renal tissue of swine in each group was obtained at PIH 48, and the pathologic changes were observed by optical microscopy and electron microscope. Data were processed with analysis of variance of repeated measurement, one-way analysis of variance, and LSD test.Results:(1) The hemodynamic indexes of swine in each group were similar before injury and at the first and second PIH 24 (withPvalues above 0.05). Compared with those before injury, except that the heart rate of swine in group A had no significant change at the first PIH 24 (P>0.05), the heart rate of swine in each group was significantly increased at the first and second PIH 24 (withPvalues below 0.01); except that the systolic blood pressure of swine in group P was significantly increased at the first and second PIH 24 (P<0.05 orP<0.01), there were no significant changes of systolic blood pressure and diastolic blood pressure of swine in each group at the first and second PIH 24 (withPvalues above 0.05); except that urine volume of swine in groups S and A was significantly decreased at the first PIH 24 (P<0.05 orP<0.01), there were no significant change of urine volume of swine in each group at the first and second PIH 24 (withPvalues above 0.05); pulmonary capillary wedge pressure and central venous pressure of swine in each group were significantly increased at the first and second PIH 24 (P<0.05 orP<0.01). (2) Compared with that in group A, the volume of resuscitation fluid of swine in groups S and H had no significant change in the first and second PIH 24 (withPvalues above 0.05), while it was significantly increased in group P in the first PIH 24 and significantly decreased in the second PIH 24 (withPvalues below 0.05). (3) Compared with those in group A, except that serum creatinine of swine in group H was significantly increased at PIH 24 and significantly increased in group P at PIH 4, 8, 24, and 48, urea nitrogen of swine in group P was significantly decreased at PIH 4 and 8 and significantly increased at PIH 48, the ratio of urine microalbumin to urine creatinine of swine in group P was significantly increased at PIH 8, 24, and 48 (P<0.05 orP<0.01), serum creatinine, urea nitrogen, and the ratio of urine microalbumin to urine creatinine of swine in each group had no significant change at each time point (withPvalues above 0.05). Serum creatinine of swine in group P was (125±16) μmol/L at PIH 24, which was significantly higher than that before injury [(75±13) μmol/L,P<0.05]. Urea nitrogen of swine in group S was (2.90±1.17) μmol/L at PIH 48, which was significantly lower than that before injury [(4.60±0.47) μmol/L,P<0.05]; urea nitrogen of swine in group H was (4.82±0.82) μmol/L at PIH 4, which was significantly higher than that before injury [(3.80±0.73) μmol/L,P<0.05]; urea nitrogen values of swine in group A were respectively (4.80±0.33), (4.92±0.35), and (2.60±0.27) μmol/L at PIH 4, 8, and 48, while those at PIH 4, 8 were significantly higher and at PIH 48 was significantly lower than the value before injury [(3.93±0.32) μmol/L, withPvalues below 0.01]. The ratios of urine microalbumin to urine creatinine of swine in group P were respectively (106.7±16.4) and (171.6±36.9) mg/mmol at PIH 24 and 48, which were significantly higher than the ratio before injury [(59.0±3.0) mg/mmol, withPvalues below 0.01]. The serum creatinine, urea nitrogen, and the ratio of urine microalbumin to urine creatinine of swine in each group at the other time points were similar to those before injury (withPvalues above 0.05). (4) The renal tissue of swine in the four groups had no obvious pathological change.Conclusions:According to the renal function results, fluid resuscitation with electrolyte and colloids are better than with lactated Ringer's solution in swine during shock stage of burn injury, while natural colloids and succinylated gelatin have similar effects, and both are superior to hydroxyethyl starch 130/0.4.
authors.affiliation: Burns and Skin Repair Surgery, the Third Affiliated Hospital of Whenzhou Medical University, Ruian 325200, China.
authors.forename: G L
authors.initials: GL
authors.lastname: Su
authors.forename: W X
authors.initials: WX
authors.lastname: Huang
authors.forename: J
authors.initials: J
authors.lastname: Chen
authors.forename: D J
authors.initials: DJ
authors.lastname: Xue
authors.forename: J J
authors.initials: JJ
authors.lastname: Zhou
mesh_terms: D000818:Animals; D001794:Blood Pressure; D001830:Body Surface Area; D002056:Burns / Q000150:complications*; D003102:Colloids; D005440:Fluid Therapy; D006895:Hydroxyethyl Starch Derivatives; D007552:Isotonic Solutions; D012151:Resuscitation; D000077325:Ringer's Lactate; D012769:Shock; D013552:Swine
chemical_list: D003102:Colloids; D006895:Hydroxyethyl Starch Derivatives; D007552:Isotonic Solutions; D000077325:Ringer's Lactate