title: Hsa_circ_0054633 in peripheral blood can be used as a diagnostic biomarker of pre-diabetes and type 2 diabetes mellitus.
abstract: OBJECTIVE
  The purpose of the current study was to investigate the characteristic expression of circular RNAs (circRNAs) in the peripheral blood of type 2 diabetes mellitus (T2DM) patients and their potential as diagnostic biomarkers for pre-diabetes and T2DM.
  
  
  METHODS
  CircRNAs in the peripheral blood from six healthy individuals and six T2DM patients were collected for microarray analysis, and an independent cohort study consisting of 20 normal cases, 20 pre-diabetes patients and 20 T2DM patients was conducted to verify the five chosen circRNAs. We then tested hsa_circ_0054633 in a third cohort (control group, n = 60; pre-diabetes group, n = 63; and T2DM group, n = 64) by quantitative real-time polymerase chain reaction (Q-PCR).
  
  
  RESULTS
  In total, 489 circRNAs were discovered to be differentially expressed between the two groups, and of these, 78 were upregulated and 411 were downregulated in the T2DM group. Five circRNAs were then selected as candidate biomarkers and further verified in a second cohort. Hsa_circ_0054633 was found to have the largest area under the curve (AUC). The diagnostic capacity of hsa_circ_0054633 was tested in a third cohort. After introducing the risk factors of T2DM, the hsa_circ_0054633 AUCs for the diagnosis of pre-diabetes and T2DM slightly increased from 0.751 (95% confidence interval [0.666-0.835], P < 0.001) to 0.841 ([0.773-0.910], P < 0.001) and from 0.793 ([0.716-0.871], P < 0.001) to 0.834 ([0.762-0.905], P < 0.001), respectively.
  
  
  CONCLUSIONS
  Hsa_circ_0054633 presented a certain diagnostic capability for pre-diabetes and T2DM.
authors.affiliation: Department of Cardiology, People's Hospital of Zhengzhou University, Zhengzhou University, Zhengzhou, China.
authors.forename: Zhenzhou
authors.initials: Z
authors.lastname: Zhao
authors.affiliation: Department of Cardiology, People's Hospital of Zhengzhou University, Zhengzhou University, Zhengzhou, China.
authors.forename: Xuejie
authors.initials: X
authors.lastname: Li
authors.affiliation: Department of Cardiology, The First Affiliated Hospital of Zhejiang University, Zhejiang University, Hangzhou, China.
authors.forename: Dongdong
authors.initials: D
authors.lastname: Jian
authors.affiliation: Department of Cardiology, People's Hospital of Zhengzhou University, Zhengzhou University, Zhengzhou, China.
authors.forename: Peiyuan
authors.initials: P
authors.lastname: Hao
authors.affiliation: Department of Cardiology, People's Hospital of Zhengzhou University, Zhengzhou University, Zhengzhou, China.
authors.forename: Lixin
authors.initials: L
authors.lastname: Rao
authors.affiliation: Department of Cardiology, People's Hospital of Zhengzhou University, Zhengzhou University, Zhengzhou, China. limuwei@medmail.com.cn.
authors.forename: Muwei
authors.initials: M
authors.lastname: Li
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015415:Biomarkers / Q000097:blood; D016022:Case-Control Studies; D015331:Cohort Studies; D003924:Diabetes Mellitus, Type 2 / Q000097:blood* / Q000175:diagnosis* / Q000235:genetics; D015536:Down-Regulation; D005260:Female; D006801:Humans; D008297:Male; D046228:Microarray Analysis; D008875:Middle Aged; D011236:Prediabetic State / Q000097:blood* / Q000175:diagnosis* / Q000235:genetics; D011379:Prognosis; D012313:RNA / Q000097:blood*; D000079962:RNA, Circular; D012680:Sensitivity and Specificity
chemical_list: D015415:Biomarkers; D000079962:RNA, Circular; D012313:RNA
keywords: Biomarker; Circular RNAs (circRNAs); Circulating circRNA; Microarray analysis; Pre-diabetes; Type 2 diabetes mellitus (T2DM)