title: Anti-tumor and immunomodulatory activities induced by an alkali-extracted polysaccharide BCAP-1 from Bupleurum chinense via NF-κB signaling pathway.
abstract: Bupleurum chinense is a well-known traditional Chinese medicine. Polysaccharides extracted from medical plants possess multiple healthy benefits. In the present study, an alkali-extracted polysaccharide (BCAP-1) was isolated from Bupleurum chinense, and evaluated its physicochemical features, anti-tumor activities and immunomodulatory effects. BCAP-1 was obtained by alkali-extraction, ethanol precipitation, and fractionation by DEAE-cellulose and Sepharose CL-6B columns. BCAP-1 markedly inhibited Sarcoma 180tumor growth in tumor-bearing mice, and increased the secretion of TNF-α in serum. MTT assay showed that BCAP-1 had no cytotoxicity against S-180 tumor cells. BCAP-1 enhanced the secretion of TNF-α and NO, and the transcripts of TNF-α and iNOS were increased. Meanwhile, BCAP-1 treatment induced the phosphorylation of p65 and decreased the expression of IκB in macrophages. These results suggest that BCAP-1 could activate macrophages through NF-κB signaling pathway, and the anti-tumor effects of BCAP-1 can be achieved by its immunostimulating features.
authors.affiliation: School of Public Health, Jilin University, Changchun 130021, China.
authors.forename: Xiangfu
authors.initials: X
authors.lastname: Song
authors.affiliation: Jilin Provincial Key Laboratory of Molecular Geriatric Medicine, Life Science Research Center, Beihua University, Jilin 132013, China.
authors.forename: Ting
authors.initials: T
authors.lastname: Ren
authors.affiliation: Jilin Provincial Key Laboratory of Molecular Geriatric Medicine, Life Science Research Center, Beihua University, Jilin 132013, China.
authors.forename: Zhou
authors.initials: Z
authors.lastname: Zheng
authors.affiliation: School of Life Science, Jilin Agricultural University, Changchun 130118, China.
authors.forename: Tiancheng
authors.initials: T
authors.lastname: Lu
authors.affiliation: School of Public Health, Jilin University, Changchun 130021, China. Electronic address: zhicheng@jlu.edu.cn.
authors.forename: Zhicheng
authors.initials: Z
authors.lastname: Wang
authors.affiliation: College of Forestry, Beihua University, Jilin 132013, China. Electronic address: dfg4656@hotmail.com.
authors.forename: Fengguo
authors.initials: F
authors.lastname: Du
authors.affiliation: Jilin Provincial Key Laboratory of Molecular Geriatric Medicine, Life Science Research Center, Beihua University, Jilin 132013, China. Electronic address: tonghaibin@gmail.com.
authors.forename: Haibin
authors.initials: H
authors.lastname: Tong
mesh_terms: D000468:Alkalies / Q000737:chemistry*; D000818:Animals; D000970:Antineoplastic Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D029972:Bupleurum / Q000737:chemistry*; D045744:Cell Line, Tumor; D056747:Immunomodulation / Q000187:drug effects*; D008297:Male; D051379:Mice; D016328:NF-kappa B / Q000378:metabolism*; D018517:Plant Roots / Q000737:chemistry; D011134:Polysaccharides / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*
chemical_list: D000468:Alkalies; D000970:Antineoplastic Agents; D016328:NF-kappa B; D011134:Polysaccharides
keywords: Bupleurum chinense; Immunomodulation; Polysaccharide