title: New adenoviruses from new primate hosts - growing diversity reveals taxonomic weak points.
abstract: The knowledge of the closest human relatives of human adenoviruses (AdVs) such as adenoviruses found in nonhuman primates is still limited, despite the growing importance of adenoviruses in vaccine development, gene and cancer therapy. We examined 153 stool samples of 17 non-human primate species and detected adenoviral DNA sequences of DNA polymerase (DPOL) gene in 54 samples (35%), originating from 12 out of 17 primate species. We further sequenced 15 hexon gene fragments and based on the phylogenetic analysis we propose two new provisional species SAdV-H and SAdV-I. Our study shows extensive diversity of adenoviral strains forming separate clades often from closely related host species from old world monkeys suggesting the existence of new species of AdVs and shows the necessity for clear ICTV guidelines for final establishment of so far provisional AdV species.
authors.affiliation: Department of Infectious Diseases and Microbiology, University of Veterinary and Pharmaceutical Sciences Brno, Czech Republic.
authors.forename: Eva
authors.initials: E
authors.lastname: Dadáková
authors.affiliation: Institute of Soil Biology, Biology Centre, Czech Academy of Sciences, v. v. i., České Budějovice, Czech Republic.
authors.forename: Tomáš
authors.initials: T
authors.lastname: Chrudimský
authors.affiliation: Department of Infectious Diseases and Microbiology, University of Veterinary and Pharmaceutical Sciences Brno, Czech Republic.
authors.forename: Kristýna
authors.initials: K
authors.lastname: Brožová
authors.affiliation: Department of Pathology and Parasitology, University of Veterinary and Pharmaceutical Sciences Brno, Czech Republic; Institute of Parasitology, Biology Centre, Czech Academy of Sciences, Czech Republic; CEITEC VFU, University of Veterinary and Pharmaceutical Sciences Brno, Czech Republic.
authors.forename: David
authors.initials: D
authors.lastname: Modrý
authors.affiliation: Department of Infectious Diseases and Microbiology, University of Veterinary and Pharmaceutical Sciences Brno, Czech Republic; CEITEC VFU, University of Veterinary and Pharmaceutical Sciences Brno, Czech Republic.
authors.forename: Vladimír
authors.initials: V
authors.lastname: Celer
authors.affiliation: Department of Virology, Veterinary Research Institute, Czech Republic; CEITEC VFU, University of Veterinary and Pharmaceutical Sciences Brno, Czech Republic. Electronic address: kristyna@hrazdilova.cz.
authors.forename: Kristýna
authors.initials: K
authors.lastname: Hrazdilová
mesh_terms: D000261:Adenoviruses, Simian / Q000145:classification* / Q000235:genetics*; D000818:Animals; D001483:Base Sequence; D001499:Bayes Theorem; D014644:Genetic Variation; D054884:Host-Pathogen Interactions; D006801:Humans; D009711:Nucleotides / Q000235:genetics; D010802:Phylogeny; D011323:Primates / Q000821:virology*
chemical_list: D009711:Nucleotides
keywords: Adenovirus; Phylogeny; Primate; Taxonomy