title: Messages on small RNA duplexes in plants.
abstract: Small RNA-mediated gene silencing encompasses diverse developmental events, stress responses, defense against pathogens, and maintenance of genome integrity. Extensive studies in model organisms have unveiled the molecular mechanisms underpinning the RNA silencing phenomena, and the accumulating knowledge have characterized the intricate pathways and the repertoire of proteins responsible for the actions of small RNAs characterized as microRNAs (miRNAs) or small interfering RNAs (siRNAs). Although the single-stranded, matured guide small RNAs direct the effector ribonucleoprotein complexes to induce gene silencing in sequence-specific manner, the double-stranded intermediate, the small RNA duplexes, which are processed as nascent products of the RNase III enzyme activities, act as key to determine the downstream molecular pathways and the fate of small RNAs. Based at the small RNA duplex-centered view, this review describes the recent advances in understanding the small RNA pathways in plants.
authors.affiliation: Graduate School of Frontier Biosciences, Osaka University, Yamadaoka 1-3, Suita, Osaka, 565-0871, Japan. ikit@fbs.osaka-u.ac.jp.
authors.forename: Taichiro
authors.initials: T
authors.lastname: Iki
mesh_terms: D035683:MicroRNAs / Q000235:genetics*; D010944:Plants / Q000235:genetics*; D034622:RNA Interference; D018749:RNA, Plant / Q000235:genetics; D034741:RNA, Small Interfering
chemical_list: D035683:MicroRNAs; D018749:RNA, Plant; D034741:RNA, Small Interfering
keywords: ARGONAUTE (AGO); DICER LIKE (DCL); Molecular chaperone HSP90; Small RNA duplex; Small interfering RNA (siRNA); microRNA (miRNA)