title: Platelet-, leucocyte- and red cell-derived microparticles in stored whole blood, with and without leucofiltration, with and without ionising radiation.
abstract: Storage lesion, including microparticle formation, has been partially characterised in whole blood, but not in all combinations of pre-storage leucofiltration and/or irradiation.
  
  
  
  Single-donor whole blood products were processed into four subunits: with and without leucofiltration, with and without X-irradiation (25 Gy). Platelet-, leucocyte-, and erythrocyte-derived microparticles and free haemoglobin were measured periodically throughout 42 days of storage.
  
  
  
  Pre-storage leucofiltration substantially reduced platelet- and leucocyte-derived microparticle counts throughout storage. Irradiation, in contrast, had no significant effect on microparticle counts. A gate for all microparticles showed a substantial time-dependent increase in unfiltered whole blood. A time-dependent increase in free haemoglobin was greatest in unfiltered, irradiated whole blood.
  
  
  
  This study indicates that leucofiltration can prevent the formation of leucocyte- and platelet-derived microparticles, and might reduce haemolysis in irradiated whole blood, either by removing factors that provoke haemolysis, or by selective retention of senescent or effete red cells most prone to haemolysis.
authors.affiliation: Department of Blood Transfusion and Transplantation Immunology, Fukushima Medical University, Fukushima, Japan.
authors.forename: Shunnichi
authors.initials: S
authors.lastname: Saito
authors.affiliation: Department of Blood Transfusion and Transplantation Immunology, Fukushima Medical University, Fukushima, Japan.|Radiation Medical Science Center, Fukushima Medical University, Fukushima, Japan.
authors.forename: Kenneth E
authors.initials: KE
authors.lastname: Nollet
authors.affiliation: Department of Epidemiology, Biostatistics and Occupational Health, McGill University, Montreal, Quebec, Canada.
authors.forename: Alain M
authors.initials: AM
authors.lastname: Ngoma
authors.affiliation: Department of Blood Transfusion and Transplantation Immunology, Fukushima Medical University, Fukushima, Japan.
authors.forename: Takako
authors.initials: T
authors.lastname: Ono
authors.affiliation: Department of Blood Transfusion and Transplantation Immunology, Fukushima Medical University, Fukushima, Japan.|Radiation Medical Science Center, Fukushima Medical University, Fukushima, Japan.
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Ohto
mesh_terms: D000328:Adult; D001792:Blood Platelets / Q000166:cytology* / Q000378:metabolism; D001793:Blood Preservation; D055252:Cell-Derived Microparticles; D004912:Erythrocytes / Q000166:cytology* / Q000378:metabolism; D006801:Humans; D007937:Leukapheresis; D007962:Leukocytes / Q000166:cytology* / Q000378:metabolism; D008297:Male; D013997:Time Factors