title: Tissue-engineered endothelial cell layers on surface-modified Ti for inhibitingin vitroplatelet adhesion.
abstract: A tissue-engineered endothelial layer was prepared by culturing endothelial cells on a fibroblast growth factor-2 (FGF-2)-l-ascorbic acid phosphate magnesium saltn-hydrate (AsMg)-apatite (Ap) coated titanium plate. The FGF-2-AsMg-Ap coated Ti plate was prepared by immersing a Ti plate in supersaturated calcium phosphate solutions supplemented with FGF-2 and AsMg. The FGF-2-AsMg-Ap layer on the Ti plate accelerated proliferation of human umbilical vein endothelial cells (HUVECs), and showed slightly higher, but not statistically significant, nitric oxide release from HUVECs than on as-prepared Ti. The endothelial layer maintained proper function of the endothelial cells and markedly inhibitedin vitroplatelet adhesion. The tissue-engineered endothelial layer formed on the FGF-2-AsMg-Ap layer is promising for ameliorating platelet activation and thrombus formation on cardiovascular implants.
authors.affiliation: Human Technology Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Central 6, 1-1-1 Higashi, Tsukuba, Ibaraki 305-8566, Japan.
authors.forename: Xiupeng
authors.initials: X
authors.lastname: Wang
authors.affiliation: Human Technology Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Namiki1-2-1, Tsukuba, Ibaraki 305-8564, Japan; School of Materials Science and Engineering, South China University of Technology, Guangzhou 510641, People's Republic of China.
authors.forename: Fupo
authors.initials: F
authors.lastname: He
authors.affiliation: Human Technology Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Central 6, 1-1-1 Higashi, Tsukuba, Ibaraki 305-8566, Japan.
authors.forename: Xia
authors.initials: X
authors.lastname: Li
authors.affiliation: Human Technology Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Central 6, 1-1-1 Higashi, Tsukuba, Ibaraki 305-8566, Japan.
authors.forename: Atsuo
authors.initials: A
authors.lastname: Ito
authors.affiliation: Human Technology Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Central 6, 1-1-1 Higashi, Tsukuba, Ibaraki 305-8566, Japan.
authors.forename: Yu
authors.initials: Y
authors.lastname: Sogo
authors.affiliation: Human Technology Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Namiki1-2-1, Tsukuba, Ibaraki 305-8564, Japan.
authors.forename: Osamu
authors.initials: O
authors.lastname: Maruyama
authors.affiliation: Human Technology Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Namiki1-2-1, Tsukuba, Ibaraki 305-8564, Japan.
authors.forename: Ryo
authors.initials: R
authors.lastname: Kosaka
authors.affiliation: School of Materials Science and Engineering, South China University of Technology, Guangzhou 510641, People's Republic of China; National Engineering Research Center for Tissue Restoration and Reconstruction, Guangzhou 510006, People's Republic of China.
authors.forename: Jiandong
authors.initials: J
authors.lastname: Ye
keywords: 10.01; 10.03; apatite; ascorbate; endothelial cell; fibroblast growth factor-2; platelet adhesion