title: SGK3 Sensitivity of Large-Conductance Ca2+-Activated K+ Channel.
abstract: OBJECTIVE
  Large conductance Ca2+-activated K+ channels (maxi K+ channels or BK channels) are rapidly activated by increase of cytosolic Ca2+ activity. The channels participate in the regulation of diverse functions including neuronal excitation and cell volume. The BK channels may be modified by kinases. Channel regulating kinases include the serum &amp; glucocorticoid inducible kinase 3 (SGK3). The present study explored whether SGK3 modifies the activity of BK channels.
  
  
  METHODS
  cRNA encoding the Ca2+ insensitive BK channel mutant BKM513I+Δ899-903 was injected into Xenopus laevis oocytes without or with additional injection of cRNA encoding wild-type SGK3, constitutively active S419DSGK3, or catalytically inactive K191NSGK3. K+ channel activity was measured utilizing dual electrode voltage clamp.
  
  
  RESULTS
  BK channel activity in BKM513I+Δ899-903 expressing oocytes was significantly increased by co-expression of SGK3 or active S419DSGK3, but not by coexpression of inactive K191NSGK3.
  
  
  CONCLUSIONS
  SGK3 is a novel positive regulator of BK channels, and thus participates in the regulation of cell volume and excitability.
authors.affiliation: Department of Cardiology, Vascular Medicine and Physiology, University of Tübingen, Tübingen, Germany.
authors.forename: Musaab
authors.initials: M
authors.lastname: Ahmed
authors.forename: Myriam
authors.initials: M
authors.lastname: Fezai
authors.forename: Florian
authors.initials: F
authors.lastname: Lang
keywords: Cell volume; Neuronal excitation; Serum and glucocorticoid inducible kinase 3