title: Interactions of Bimodal Magnetic and Fluorescent Nanoparticles Based on Carbon Quantum Dots and Iron-Carbon Nanocomposites with Cell Cultures.
abstract: Interactions of bimodal (fluorescent and magnetic) nanoparticles with HeLa cells were studied. The nanoparticles, characterized by high magnetic moment and relaxing capacity, exhibited fluorescence sufficient for their use as labels in confocal microscopy and flow cytometry. Penetration of these nanoparticles into the cell depended on their surface charge: positively charged nanoparticles of this structure penetrated inside, while negatively charged particles were not found in the cells.
authors.affiliation: B. N. Eltsin Ural Federal University, Ekaterinburg, Russia. calamatica@gmail.com.|M. N. Mikheev Institute of Metal Physics, Ural Division of the Russian Academy of Sciences, Ekaterinburg, Russia. calamatica@gmail.com.
authors.forename: A S
authors.initials: AS
authors.lastname: Minin
authors.affiliation: B. N. Eltsin Ural Federal University, Ekaterinburg, Russia.|Institute of Immunology and Physiology, Ural Division of the Russian Academy of Sciences, Ekaterinburg, Russia.
authors.forename: A V
authors.initials: AV
authors.lastname: Belousova
authors.affiliation: Institute of Immunology and Physiology, Ural Division of the Russian Academy of Sciences, Ekaterinburg, Russia.
authors.forename: L T
authors.initials: LT
authors.lastname: Smolyuk
authors.affiliation: B. N. Eltsin Ural Federal University, Ekaterinburg, Russia.
authors.forename: M V
authors.initials: MV
authors.lastname: Ulitko
authors.affiliation: M. N. Mikheev Institute of Metal Physics, Ural Division of the Russian Academy of Sciences, Ekaterinburg, Russia.
authors.forename: M A
authors.initials: MA
authors.lastname: Uimin
authors.affiliation: M. N. Mikheev Institute of Metal Physics, Ural Division of the Russian Academy of Sciences, Ekaterinburg, Russia.
authors.forename: I V
authors.initials: IV
authors.lastname: Byzov
mesh_terms: D001692:Biological Transport; D002244:Carbon / Q000737:chemistry; D002463:Cell Membrane Permeability; D002470:Cell Survival; D005434:Flow Cytometry; D005456:Fluorescent Dyes / Q000737:chemistry / Q000378:metabolism*; D006367:HeLa Cells; D006801:Humans; D007501:Iron / Q000737:chemistry; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D018613:Microscopy, Confocal; D053761:Nanocomposites / Q000737:chemistry*; D045663:Quantum Dots / Q000737:chemistry / Q000378:metabolism*; D055672:Static Electricity; D013499:Surface Properties
chemical_list: D005456:Fluorescent Dyes; D058185:Magnetite Nanoparticles; D002244:Carbon; D007501:Iron
keywords: bimodal nanoparticles; carbon-quantum dots (CQD); cell culture; confocal microscopy; flow cytometry