title: Klp10A, a stem cell centrosome-enriched kinesin, balances asymmetries inDrosophilamale germline stem cell division.
abstract: Asymmetric stem cell division is often accompanied by stereotypical inheritance of the mother and daughter centrosomes. However, it remains unknown whether and how stem cell centrosomes are uniquely regulated and how this regulation may contribute to stem cell fate. Here we identify Klp10A, a microtubule-depolymerizing kinesin of the kinesin-13 family, as the first protein enriched in the stem cell centrosome inDrosophilamale germline stem cells (GSCs). Depletion ofklp10Aresults in abnormal elongation of the mother centrosomes in GSCs, suggesting the existence of a stem cell-specific centrosome regulation program. Concomitant with mother centrosome elongation, GSCs form asymmetric spindle, wherein the elongated mother centrosome organizes considerably larger half spindle than the other. This leads to asymmetric cell size, yielding a smaller differentiating daughter cell. We propose thatklp10Afunctions to counteract undesirable asymmetries that may result as a by-product of achieving asymmetries essential for successful stem cell divisions.
authors.affiliation: Department of Cell and Developmental Biology, Life Sciences Institute, Howard Hughes Medical Institute, University of Michigan, Ann Arbor, United States.
authors.forename: Cuie
authors.initials: C
authors.lastname: Chen
authors.affiliation: Department of Cell and Developmental Biology, Life Sciences Institute, Howard Hughes Medical Institute, University of Michigan, Ann Arbor, United States.
authors.forename: Mayu
authors.initials: M
authors.lastname: Inaba
authors.affiliation: Department of Cell and Developmental Biology, Life Sciences Institute, Howard Hughes Medical Institute, University of Michigan, Ann Arbor, United States.
authors.forename: Zsolt G
authors.initials: ZG
authors.lastname: Venkei
authors.affiliation: Department of Cell and Developmental Biology, Life Sciences Institute, Howard Hughes Medical Institute, University of Michigan, Ann Arbor, United States.
authors.forename: Yukiko M
authors.initials: YM
authors.lastname: Yamashita
mesh_terms: D000818:Animals; D002455:Cell Division; D018385:Centrosome / Q000201:enzymology*; D004330:Drosophila; D029721:Drosophila Proteins / Q000378:metabolism*; D005854:Germ Cells / Q000502:physiology*; D016547:Kinesins / Q000378:metabolism*; D008297:Male; D013234:Stem Cells / Q000502:physiology*
chemical_list: D029721:Drosophila Proteins; C481339:KLP10A protein, Drosophila; D016547:Kinesins
keywords: D. melanogaster; asymmetric cell division; centrosome; developmental biology; stem cells