title: Amperometric catechol biosensor based on laccase immobilized on nitrogen-doped ordered mesoporous carbon (N-OMC)/PVA matrix.
abstract: A functionalized nitrogen-containing ordered mesoporous carbon (N-OMC), which shows good electrical properties, was synthesized by the carbonization of polyaniline inside a SBA-15 mesoporous silica template. Based on this, through entrapping laccase onto the N-OMC/polyvinyl alcohol (PVA) film a facilely fabricated amperometric biosensor was developed. Laccase fromTrametes versicolorwas assembled on a composite film of a N-OMC/PVA modified Au electrode and the electrochemical behavior was investigated. The results indicated that the N-OMC modified electrode exhibits electrical properties towards catechol. The optimum experimental conditions of a biosensor for the detection of catechol were studied in detail. Under the optimal conditions, the sensitivity of the biosensor was 0.29 A*M-1with a detection limit of 0.31μM and a linear detection range from 0.39μM to 8.98μM for catechol. The calibration curve followed the Michaelis-Menten kinetics and the apparent Michaelis-Menten [Formula: see text] was 6.28μM. This work demonstrated that the N-OMC/PVA composite provides a suitable support for laccase immobilization and the construction of a biosensor.
authors.affiliation: College of Mechanics, Taiyuan University of Technology , Taiyuan 030024, People's Republic of China; Shanxi Key Laboratory of Material Strength and Structural Impact, Taiyuan University of Technology , Taiyuan 030024, People's Republic of China; Authors to whom any correspondence should be addressed.
authors.forename: Meiqing
authors.initials: M
authors.lastname: Guo
authors.affiliation: College of Mechanics, Taiyuan University of Technology , Taiyuan 030024, People's Republic of China; Shanxi Key Laboratory of Material Strength and Structural Impact, Taiyuan University of Technology , Taiyuan 030024, People's Republic of China.
authors.forename: Hefeng
authors.initials: H
authors.lastname: Wang
authors.affiliation: College of Mechanics, Taiyuan University of Technology , Taiyuan 030024, People's Republic of China; Shanxi Key Laboratory of Material Strength and Structural Impact, Taiyuan University of Technology , Taiyuan 030024, People's Republic of China.
authors.forename: Di
authors.initials: D
authors.lastname: Huang
authors.affiliation: College of Mechanics, Taiyuan University of Technology , Taiyuan 030024, People's Republic of China; Shanxi Key Laboratory of Material Strength and Structural Impact, Taiyuan University of Technology , Taiyuan 030024, People's Republic of China.
authors.forename: Zhijun
authors.initials: Z
authors.lastname: Han
authors.affiliation: Shanxi Coal Bed Methane Co., Ltd , Taiyuan 030024, People's Republic of China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Li
authors.affiliation: College of Mechanics, Taiyuan University of Technology , Taiyuan 030024, People's Republic of China; Shanxi Key Laboratory of Material Strength and Structural Impact, Taiyuan University of Technology , Taiyuan 030024, People's Republic of China.
authors.forename: Xiaojun
authors.initials: X
authors.lastname: Wang
authors.affiliation: Institute of Applied Mechanics and Biomedical Engineering, Taiyuan University of Technology ,Taiyuan 030024, People's Republic of China; Shanxi Key Laboratory of Material Strength and Structural Impact, Taiyuan University of Technology , Taiyuan 030024, People's Republic of China; Authors to whom any correspondence should be addressed.
authors.forename: Jing
authors.initials: J
authors.lastname: Chen
keywords: N-OMC; PVA; biosensor; electrical properties; laccase