title: Biobanking of different body fluids within the frame of IVF-a standard operating procedure to improve reproductive biology research.
abstract: OBJECTIVE
  The aim of the present study was to develop a standard operating procedure (SOP) for the collection, transport, and storage of human cumulus cells, follicular fluid, blood serum, seminal plasma, embryo culture supernatant, and embryo culture supernatant control obtained within the IVF process under approved protocols and written informed consent from participating patients. The SOP was developed at the Kinderwunsch Institut Schenk, Dobl, Austria, together with Biobank Graz of the Medical University of Graz, Austria.
  
  
  METHODS
  The SOP provides comprehensive details of laboratory procedures and sampling of the different fluids within the IVF process. Furthermore, information on sample coding, references of involved laboratory techniques (e.g., oocyte retrieval with a Steiner-TAN needle), ethical approvals, and biobanking procedures are presented.
  
  
  RESULTS
  The result of the present study is a standard operating procedure.
  
  
  CONCLUSIONS
  The SOP ensures a professional way for collection and scientific use of IVF samples by the Kinderwunsch Institut Schenk, Dobl, Austria, and Biobank Graz of the Medical University of Graz, Austria. It can be used as a template for other institutions to unify specimen collection procedures in the field of reproductive health research.
authors.affiliation: Das Kinderwunsch Institut Schenk GmbH, Am Sendergrund 11, 8143, Dobl, Austria.
authors.forename: Michael
authors.initials: M
authors.lastname: Schenk
authors.affiliation: Biobank Graz, Medical University of Graz, Neue Stiftingtalstrasse 2, 8010, Graz, Austria.
authors.forename: Berthold
authors.initials: B
authors.lastname: Huppertz
authors.affiliation: Division of Endocrinology and Diabetology, Department of Internal Medicine, Medical University of Graz, Auenbruggerplatz 15, 8036, Graz, Austria.
authors.forename: Barbara
authors.initials: B
authors.lastname: Obermayer-Pietsch
authors.affiliation: Das Kinderwunsch Institut Schenk GmbH, Am Sendergrund 11, 8143, Dobl, Austria.
authors.forename: Darja
authors.initials: D
authors.lastname: Kastelic
authors.affiliation: Das Kinderwunsch Institut Schenk GmbH, Am Sendergrund 11, 8143, Dobl, Austria.
authors.forename: Martina
authors.initials: M
authors.lastname: Hörmann-Kröpfl
authors.affiliation: Das Kinderwunsch Institut Schenk GmbH, Am Sendergrund 11, 8143, Dobl, Austria. gregor.weiss@kinderwunsch-institut.at.
authors.forename: Gregor
authors.identifier: http://orcid.org/0000-0003-1045-8595
authors.initials: G
authors.lastname: Weiss
mesh_terms: D000328:Adult; D018070:Biological Specimen Banks; D001826:Body Fluids / Q000166:cytology*; D054885:Cumulus Cells / Q000166:cytology; D046149:Embryo Culture Techniques; D005260:Female; D005307:Fertilization in Vitro; D015571:Follicular Fluid / Q000166:cytology; D006801:Humans; D054315:Oocyte Retrieval; D027724:Reproductive Techniques, Assisted; D012661:Semen / Q000166:cytology; D044967:Serum / Q000166:cytology
keywords: Biobanking; Follicular fluid; IVF; Standard operating procedure; Steiner-TAN needle