title: A Conserved Puccinia striiformis Protein Interacts with Wheat NPR1 and Reduces Induction of Pathogenesis-Related Genes in Response to Pathogens.
abstract: In Arabidopsis, NPR1 is a key transcriptional coregulator of systemic acquired resistance. Upon pathogen challenge, NPR1 translocates from the cytoplasm to the nucleus, in which it interacts with TGA-bZIP transcription factors to activate the expression of several pathogenesis-related (PR) genes. In a screen of a yeast two-hybrid library from wheat leaves infected with Puccinia striiformis f. sp. tritici, we identified a conserved rust protein that interacts with wheat NPR1 and named it PNPi (for Puccinia NPR1 interactor). PNPi interacts with the NPR1/NIM1-like domain of NPR1 via its C-terminal DPBB_1 domain. Using bimolecular fluorescence complementation assays, we detected the interaction between PNPi and wheat NPR1 in the nucleus of Nicotiana benthamiana protoplasts. A yeast three-hybrid assay showed that PNPi interaction with NPR1 competes with the interaction between wheat NPR1 and TGA2.2. In barley transgenic lines overexpressing PNPi, we observed reduced induction of multiple PR genes in the region adjacent to Pseudomonas syringae pv. tomato DC3000 infection. Based on these results, we hypothesize that PNPi has a role in manipulating wheat defense response via its interactions with NPR1.
authors.affiliation: 1 Department of Plant Science, University of California, Davis, CA 95616, U.S.A.|2 State Key Laboratory of Crop Stress Biology for Arid Areas and College of Plant Protection, Northwest Agriculture and Forestry University, Yangling, Shaanxi 712100, P. R. China.|3 College of Plant Protection, Biological Control Center for Plant Diseases and Plant Pests of Hebei, Agriculture University of Hebei, Baoding, Hebei 071000, P. R. China.
authors.forename: Xiaodong
authors.initials: X
authors.lastname: Wang
authors.affiliation: 1 Department of Plant Science, University of California, Davis, CA 95616, U.S.A.
authors.forename: Baoju
authors.initials: B
authors.lastname: Yang
authors.affiliation: 1 Department of Plant Science, University of California, Davis, CA 95616, U.S.A.
authors.forename: Kun
authors.initials: K
authors.lastname: Li
authors.affiliation: 2 State Key Laboratory of Crop Stress Biology for Arid Areas and College of Plant Protection, Northwest Agriculture and Forestry University, Yangling, Shaanxi 712100, P. R. China.
authors.forename: Zhensheng
authors.initials: Z
authors.lastname: Kang
authors.affiliation: 4 Department of Viticulture and Enology, University of California, Davis, CA 95616, U.S.A.
authors.forename: Dario
authors.initials: D
authors.lastname: Cantu
authors.affiliation: 1 Department of Plant Science, University of California, Davis, CA 95616, U.S.A.|5 Howard Hughes Medical Institute (HHMI), Chevy Chase, MD 20815, U.S.A.
authors.forename: Jorge
authors.initials: J
authors.lastname: Dubcovsky
mesh_terms: D000595:Amino Acid Sequence; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism; D050976:Basic-Leucine Zipper Transcription Factors / Q000235:genetics / Q000378:metabolism; D001487:Basidiomycota / Q000235:genetics / Q000502:physiology*; D060467:Disease Resistance; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D015723:Gene Library; D001467:Hordeum / Q000235:genetics / Q000276:immunology / Q000382:microbiology; D010935:Plant Diseases / Q000276:immunology* / Q000382:microbiology; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D016415:Sequence Alignment; D014908:Triticum / Q000235:genetics / Q000276:immunology* / Q000382:microbiology; D020798:Two-Hybrid System Techniques
chemical_list: D029681:Arabidopsis Proteins; D050976:Basic-Leucine Zipper Transcription Factors; D005656:Fungal Proteins; C105496:NPR1 protein, Arabidopsis; D010940:Plant Proteins; C053376:pathogenesis-related proteins, plant