title: Genomic characterization of two new enterovirus types, EV-A114 and EV-A121.
abstract: Enteroviruses cause a variety of illnesses of the gastrointestinal tract, central nervous system and cardiovascular system. Phylogenetic analysis of VP1 sequences has identified 106 different human enteroviruses classified into four enterovirus species within the genus Enterovirus of the family Picornaviridae. It is likely that not all enterovirus types have been discovered. Between September 2013 and October 2014, stool samples of 6274 apparently healthy children of up to 5 years of age residing in Gorakhpur district, Uttar Pradesh, India were screened for enteroviruses. Virus isolates obtained in RD and Hep-2c cells were identified by complete VP1 sequencing. Enteroviruses were isolated from 3042 samples. A total of 87 different enterovirus types were identified. Two isolates with 71 and 74 % nucleotide sequence similarity to all other known enteroviruses were recognized as novel types. In this paper we report identification and complete genome sequence analysis of these two isolates classified as EV-A114 and EV-A121.
authors.affiliation: Enterovirus Research Centre, Haffkine Institute Compound, AD Marg, Parel, Mumbai, Maharashtra 400012, India.
authors.forename: Jagadish M
authors.initials: JM
authors.lastname: Deshpande
authors.affiliation: Enterovirus Research Centre, Haffkine Institute Compound, AD Marg, Parel, Mumbai, Maharashtra 400012, India.
authors.forename: Deepa K
authors.initials: DK
authors.lastname: Sharma
authors.affiliation: Enterovirus Research Centre, Haffkine Institute Compound, AD Marg, Parel, Mumbai, Maharashtra 400012, India.
authors.forename: Vinay K
authors.initials: VK
authors.lastname: Saxena
authors.affiliation: Enterovirus Research Centre, Haffkine Institute Compound, AD Marg, Parel, Mumbai, Maharashtra 400012, India.
authors.forename: Sushmitha A
authors.initials: SA
authors.lastname: Shetty
authors.affiliation: Enterovirus Research Centre, Haffkine Institute Compound, AD Marg, Parel, Mumbai, Maharashtra 400012, India.
authors.forename: Tarique Husain I H
authors.initials: THIH
authors.lastname: Qureshi
authors.affiliation: Enterovirus Research Centre, Haffkine Institute Compound, AD Marg, Parel, Mumbai, Maharashtra 400012, India.
authors.forename: Uma P
authors.initials: UP
authors.lastname: Nalavade
mesh_terms: D002675:Child, Preschool; D029821:Enterovirus A, Human / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D029822:Enterovirus B, Human; D004769:Enterovirus Infections / Q000453:epidemiology / Q000821:virology*; D005243:Feces / Q000821:virology; D005260:Female; D016679:Genome, Viral; D006801:Humans; D007194:India / Q000453:epidemiology; D007223:Infant; D008297:Male; D010802:Phylogeny; D012367:RNA, Viral; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D013045:Species Specificity; D015678:Viral Structural Proteins / Q000235:genetics
chemical_list: D012367:RNA, Viral; D015678:Viral Structural Proteins