title: Chromosome 16q genesCDH1, CDH13andADAMTS18are correlated and frequently methylated in human lymphoma.
abstract: The products of the E-cadherin (CDH1), H-cadherin (CDH13) and a disintegrin and metalloproteinase with thrombospondin motif 18 (ADAMTS18) genes are proteins displaying structural features and functions on the cell surface membrane, and have been reported to be involved in cancer progression. Using reverse transcription-quantitative polymerase chain reaction (RT-qPCR) and methylation-specific PCR (MSP) analysis, the promoter methylation status and messenger RNA (mRNA) expression levels ofCDH1,CDH13andADAMTS18, which are putative tumor-suppressor genes located on chromosome 16q, were evaluated. In addition, the mRNA expression levels of DNA methyltransferases (DNMTs) 1, 3A and3Bwere examined, and the correlations among the different parameters analyzed were studied in 36 lymphomas and 16 non-malignant lymphoid tissue samples. A significant positive correlation was identified between the expression levels ofCDH1andCDH13(r=0.735, P<0.01).ADAMTS18expression also exhibited a significant positive correlation with bothCDH1andCDH13mRNA expression levels (r=0.625, P<0.01; andr=0.720, P<0.01, respectively). Our results indicated thatCDH1,CDH13andADAMTS18, which are localized on chromosome 16q, are remarkably correlated and frequently methylated in human lymphomas, and their methylation could not be explained solely by the mRNA expression level ofDNMTs.
authors.affiliation: Department of Laboratory Sciences, Graduate School of Health Sciences, Gunma University, Gunma 371-8511, Japan.
authors.forename: Lobna
authors.initials: L
authors.lastname: Alkebsi
authors.affiliation: Department of Medicine and Clinical Sciences, Graduate School of Medicine, Gunma University Hospital, Gunma 371-8511, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Handa
authors.affiliation: Blood Transfusion Service, Gunma University Hospital, Gunma 371-8511, Japan.
authors.forename: Akihiko
authors.initials: A
authors.lastname: Yokohama
authors.affiliation: Oncology Center, Gunma University Hospital, Gunma 371-8511, Japan.
authors.forename: Takayuki
authors.initials: T
authors.lastname: Saitoh
authors.affiliation: Oncology Center, Gunma University Hospital, Gunma 371-8511, Japan.
authors.forename: Norifumi
authors.initials: N
authors.lastname: Tsukamoto
authors.affiliation: Department of Laboratory Sciences, Graduate School of Health Sciences, Gunma University, Gunma 371-8511, Japan.
authors.forename: Hirokazu
authors.initials: H
authors.lastname: Murakami
keywords: ADAMTS18; CDH1; CDH13; human lymphoma