title: Sequence and Phylogenetic Analyses of the Nsp2 and ORF5 Genes of Porcine Reproductive and Respiratory Syndrome Virus in Boars from South China in 2015.
abstract: Porcine reproductive and respiratory syndrome virus (PRRSV) is highly genetically diverse; however, little is known about the molecular epidemiology of PRRSV in the boar farms of South China. In this study, 367 samples were collected from boar farms in South China in 2015. The Nsp2 hypervariable region and ORF5 gene were PCR amplified from 66 PRRSV-positive samples, followed by sequencing and analysis. The percentage of PRRSV antigen-positive samples was 17.98%; 8.72% were positive for highly pathogenic PRRSV (HP-PRRSV), and 9.26% were positive for low pathogenic PRRSV (LP-PRRSV). Sequence alignment and phylogenetic tree analyses revealed three novel patterns of deletion in the hypervariable region of Nsp2, which had not been identified previously. Furthermore, numerous amino acid substitutions were identified in the putative signal peptide and extravirion regions of GP5. These results demonstrate for the first time that the existence of multiple different strains on the same boar farm, and extensive genetic mutation and high infection rate of PRRSV in boars from South China. Our research contributes to the understanding of the epidemiology and genetic characteristics of PRRSV on boar farms.
authors.affiliation: College of Animal Science & National Engineering Center for Swine Breeding Industry, South China Agriculture University, Guangzhou, China.
authors.forename: P P
authors.initials: PP
authors.lastname: Wang
authors.affiliation: College of Animal Science & National Engineering Center for Swine Breeding Industry, South China Agriculture University, Guangzhou, China.|Xinyang Animal Disease Prevention and Control Engineering Research Center, Xinyang College of Agriculture and Forestry, Xinyang, China.
authors.forename: J G
authors.initials: JG
authors.lastname: Dong
authors.affiliation: College of Animal Science & National Engineering Center for Swine Breeding Industry, South China Agriculture University, Guangzhou, China.
authors.forename: L Y
authors.initials: LY
authors.lastname: Zhang
authors.affiliation: College of Animal Science & National Engineering Center for Swine Breeding Industry, South China Agriculture University, Guangzhou, China.
authors.forename: P S
authors.initials: PS
authors.lastname: Liang
authors.affiliation: College of Animal Science & National Engineering Center for Swine Breeding Industry, South China Agriculture University, Guangzhou, China.
authors.forename: Y L
authors.initials: YL
authors.lastname: Liu
authors.affiliation: College of Animal Science & National Engineering Center for Swine Breeding Industry, South China Agriculture University, Guangzhou, China.
authors.forename: L
authors.initials: L
authors.lastname: Wang
authors.affiliation: Testing Center of Breeding Swine Quality of China Ministry of Agriculture, Guangzhou, China.
authors.forename: F H
authors.initials: FH
authors.lastname: Fan
authors.affiliation: College of Animal Science & National Engineering Center for Swine Breeding Industry, South China Agriculture University, Guangzhou, China.
authors.forename: C X
authors.initials: CX
authors.lastname: Song
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002681:China; D019316:Porcine respiratory and reproductive syndrome virus / Q000235:genetics*; D016415:Sequence Alignment / Q000662:veterinary; D034421:Sus scrofa / Q000821:virology*; D014759:Viral Envelope Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D017361:Viral Nonstructural Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism
chemical_list: D014759:Viral Envelope Proteins; D017361:Viral Nonstructural Proteins; C114789:glycoprotein 5, PRRSV
keywords: GP5; Nsp2; Porcine reproductive and respiratory syndrome virus; mutation; phylogenetic analysis