title: Mesenchymal-Epithelial Transition in Culture of Stromal Progenitor Cells Isolated from the Liver of a Patient with Alcoholic Cirrhosis.
abstract: The cells isolated from biopsy specimen of a patient with alcoholic liver cirrhosis and cultured under standard conditions for obtaining stromal cell culture clearly diverged during early passages into two morphologically and phenotypically different subtypes: epithelial and mesenchymal. Mesenchymal cells expressed CD90 and CD44 and epithelial cells expressed CD166, CD227, and hepatocyte growth factor receptor Met. Starting from passage 6, the culture underwent spontaneous morphological changes and by passages 8-10 contained only epithelium-like cells. CD90 and CD44 expression disappeared, CD166 and CD227 expression remained unchanged, and Met expression increased. A small fraction of cells expressed GATA-4, HNF3β, HNF1α, and HNF4α. After addition of inducers of hepatogeneic differentiation, the cells started producing albumin.
authors.affiliation: V. N. Orekhovich Research Institute of Biomedical Chemistry, Moscow, Russia. irkhol@yandex.ru.
authors.forename: I V
authors.initials: IV
authors.lastname: Kholodenko
authors.affiliation: M. M. Shemyakin and Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia.
authors.forename: R V
authors.initials: RV
authors.lastname: Kholodenko
authors.affiliation: B. V. Petrovsky Russian National Research Center of Surgery, Russian Academy of Medical Sciences, Moscow, Russia.
authors.forename: G V
authors.initials: GV
authors.lastname: Manukyan
authors.affiliation: V. N. Orekhovich Research Institute of Biomedical Chemistry, Moscow, Russia.
authors.forename: V V
authors.initials: VV
authors.lastname: Burunova
authors.affiliation: V. N. Orekhovich Research Institute of Biomedical Chemistry, Moscow, Russia.
authors.forename: K N
authors.initials: KN
authors.lastname: Yarygin
mesh_terms: D000418:Albumins / Q000096:biosynthesis / Q000235:genetics; D015703:Antigens, CD / Q000235:genetics / Q000378:metabolism; D015415:Biomarkers / Q000378:metabolism; D002454:Cell Differentiation; D049109:Cell Proliferation; D058750:Epithelial-Mesenchymal Transition / Q000235:genetics*; D050991:GATA4 Transcription Factor / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D051538:Hepatocyte Nuclear Factor 1-alpha / Q000235:genetics / Q000378:metabolism; D051542:Hepatocyte Nuclear Factor 3-beta / Q000235:genetics / Q000378:metabolism; D051557:Hepatocyte Nuclear Factor 4 / Q000235:genetics / Q000378:metabolism; D006801:Humans; D008099:Liver / Q000378:metabolism* / Q000473:pathology; D008104:Liver Cirrhosis, Alcoholic / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D059630:Mesenchymal Stem Cells / Q000378:metabolism* / Q000473:pathology; D061251:Primary Cell Culture; D019859:Proto-Oncogene Proteins c-met / Q000235:genetics / Q000378:metabolism; D013234:Stem Cells / Q000378:metabolism* / Q000473:pathology
chemical_list: D000418:Albumins; D015703:Antigens, CD; D015415:Biomarkers; C496502:FOXA2 protein, human; D050991:GATA4 Transcription Factor; C484170:GATA4 protein, human; C496487:HNF1A protein, human; C496592:HNF4A protein, human; D051538:Hepatocyte Nuclear Factor 1-alpha; D051557:Hepatocyte Nuclear Factor 4; D051542:Hepatocyte Nuclear Factor 3-beta; C491743:MET protein, human; D019859:Proto-Oncogene Proteins c-met
keywords: hepatogeneic differentiation; liver stromal progenitor cells; mesenchymal-epithelial transition