title: Novel "extended sequons" of human N-glycosylation sites improve the precision of qualitative predictions: an alignment-free study of pattern recognition using ProtDCal protein features.
abstract: N-Glycosylation is a common post-translational modification that plays an important role in the proper folding and function of many proteins. This modification is largely dependent on the presence of a sequence motif called a "sequon" defined as Asn-Xxx-Ser/Thr. However, evidence has shown that the presence of such a "sequon" is insufficient to determine the occurrence of N-glycosylation with high precision. This study aims to elucidate patterns that can more accurately predict N-glycosylation sites in human proteins. The novel motifs are evaluated using benchmarking data from 188 organisms. Performance is largely sustained compared to the human data, which validates the robustness of the novel extracted "extended sequons". We, therefore, introduce new knowledge about sequence-related factors that control N-glycosylation.
authors.affiliation: Facultad de Química y Farmacia, Universidad Central "Marta Abreu" de Las Villas, Villa Clara, Santa Clara, Cuba. yasserblanco@sce.carleton.ca.|Theoretical Chemistry, Max-Planck-Institut für Kohlenforschung, Mülheim an der Rhur, Germany. yasserblanco@sce.carleton.ca.
authors.forename: Yasser B
authors.initials: YB
authors.lastname: Ruiz-Blanco
authors.affiliation: Universidad San Francisco de Quito (USFQ), Grupo de Medicina Molecular y Traslacional (MeM&T), Colegio de Ciencias de la Salud (COCSA), Escuela de MedicinaEcuador; and Grupo de Investigación Microbiología y Ambiente (GIMA), Programa de Bacteriología, Facultad Ciencias de la Salud, Universidad de San Buenaventura, Cartagena (Bolívar), Colombia.
authors.forename: Yovani
authors.initials: Y
authors.lastname: Marrero-Ponce
authors.affiliation: Instituto de Química, Universidad Nacional Autónoma de México, Circuito Exterior, Ciudad Universitaria., México, DF, 04360, Mexico.
authors.forename: Enrique
authors.initials: E
authors.lastname: García-Hernández
authors.affiliation: Department of Systems and Computer Engineering, Carleton University, Ottawa, Canada.
authors.forename: James
authors.initials: J
authors.lastname: Green
mesh_terms: D000465:Algorithms; D030562:Databases, Protein; D006031:Glycosylation; D006801:Humans; D011499:Protein Processing, Post-Translational; D011506:Proteins / Q000737:chemistry / Q000378:metabolism*; D012984:Software
chemical_list: D011506:Proteins
keywords: Glycosylation motif; PTM; Post-translational modification; Protein descriptor