title: A benzimidazole derivative (RCB20) in vitro induces an activation of energetic pathways on Taenia crassiceps (ORF strain) cysticerci.
abstract: Human cysticercosis caused by Taenia crassiceps is unusual; however, it is an useful experimental model for cysticercosis studies. Benzimidazole derivatives are important antihelminthic drugs widely used against helminths. A novel compound 6-chloro-5-(1-naphthyloxy) -2-(trifluoromethyl)-1H-benzimidazole (RCB20) is a benzimidazole derivative less polar and more lipophilic. The aim of this study was to detect the effect of the RCB20 on the in vitro energetic metabolism of T. crassiceps cysticerci. For this, products of the metabolism both produced and secreted/excreted (S/E) by the parasite were detected through spectrophotometry and high performance liquid chromatography after exposure to 6.5 and 13 μM of RCB20 and albendazole sulfoxide (ABZSO). There was a gradual increase in the concentrations of glucose not uptaken by parasites exposed to both concentrations RCB20 and ABZSO. There was a higher concentration of all the organic acids related to the tricarboxilic acid cycle int the parasites exposed to RCB20. The structural differences between RCB20 and ABZSO result in different targets within the parasite and in a greater induction of the energetic pathways, such as the glycolysis and the TCA cycle. RCB20 is a good candidate as a substitute for anthelminthic benzimidazoles due to a differentiated site of action with similar outcome.
authors.affiliation: Laboratory of Studies of the Host-parasite Relationship, Tropical Pathology and Public Health Institute, Federal University of Goias, Brazil. Electronic address: carolinamfraga@gmail.com.
authors.forename: Carolina Miguel
authors.initials: CM
authors.lastname: Fraga
authors.affiliation: Laboratory of Studies of the Host-parasite Relationship, Tropical Pathology and Public Health Institute, Federal University of Goias, Brazil. Electronic address: tatiane_luiza@yahoo.com.br.
authors.forename: Tatiane Luiza
authors.initials: TL
authors.lastname: da Costa
authors.affiliation: Laboratory of Studies of the Host-parasite Relationship, Tropical Pathology and Public Health Institute, Federal University of Goias, Brazil. Electronic address: amaria.ana@gmail.com.
authors.forename: Ana Maria
authors.initials: AM
authors.lastname: de Castro
authors.affiliation: Laboratorio de Biologia del Citoesqueleto, Departamento de Microbiologia y Parasitologia, Facultad de Medicina, Universidad Nacional Autonoma de Mexico (UNAM), México, DF 04510, Mexico. Electronic address: oard_2000@hotmail.com.
authors.forename: Olivia
authors.initials: O
authors.lastname: Reynoso-Ducoing
authors.affiliation: Laboratorio de Biologia del Citoesqueleto, Departamento de Microbiologia y Parasitologia, Facultad de Medicina, Universidad Nacional Autonoma de Mexico (UNAM), México, DF 04510, Mexico. Electronic address: jrah@unam.mx.
authors.forename: Javier
authors.initials: J
authors.lastname: Ambrosio
authors.affiliation: Facultad de Química, Departamento de Farmacia, Universidad Nacional Autónoma de México (UNAM), México, DF 04510, Mexico. Electronic address: hercam@unam.mx.
authors.forename: Alicia
authors.initials: A
authors.lastname: Hernández-Campos
authors.affiliation: Facultad de Química, Departamento de Farmacia, Universidad Nacional Autónoma de México (UNAM), México, DF 04510, Mexico. Electronic address: rafaelc@unam.mx.
authors.forename: Rafael
authors.initials: R
authors.lastname: Castillo
authors.affiliation: Laboratory of Studies of the Host-parasite Relationship, Tropical Pathology and Public Health Institute, Federal University of Goias, Brazil. Electronic address: marinavinaud@gmail.com.
authors.forename: Marina Clare
authors.initials: MC
authors.lastname: Vinaud
mesh_terms: D015766:Albendazole / Q000031:analogs & derivatives* / Q000494:pharmacology; D000818:Animals; D000923:Anticestodal Agents / Q000494:pharmacology*; D001562:Benzimidazoles / Q000494:pharmacology*; D002952:Citric Acid Cycle / Q000187:drug effects*; D003552:Cysticercus / Q000187:drug effects* / Q000378:metabolism*; D004734:Energy Metabolism / Q000187:drug effects*; D005947:Glucose / Q000378:metabolism; D006019:Glycolysis / Q000187:drug effects
chemical_list: C557917:6-chloro-5-(1-naphthyloxy)-2-(trifluoromethyl)-1H-benzimidazole; D000923:Anticestodal Agents; D001562:Benzimidazoles; D015766:Albendazole; D005947:Glucose; C027186:albendazole sulfoxide
keywords: Albendazole sulfoxide; Energetic metabolism; RCB20; Taenia crassiceps