title: Fiber-optic electron-spin-resonance thermometry of single laser-activated neurons.
abstract: Optically detected electron spin resonance in fiber-coupled nitrogen-vacancy (NV) centers of diamond is used to demonstrate a fiber-optic quantum thermometry of individual thermogenetically activated neurons. Laser-induced temperature variations read out from single neurons with the NV-diamond fiber sensor are shown to strongly correlate with the fluorescence of calcium-ion sensors, serving as online indicators of the inward Ca2+current across the cell membrane of neurons expressing transient receptor potential (TRP) cation channels. Local laser heating above the TRP-channel activation threshold is shown to reproducibly evoke robust action potentials, visualized by calcium-ion-sensor-aided fluorescence imaging and detected as prominent characteristic waveforms in the time-resolved response of fluorescence Ca2+sensors.
authors.forename: A A
authors.initials: AA
authors.lastname: Lanin
authors.forename: I V
authors.initials: IV
authors.lastname: Fedotov
authors.forename: Yu G
authors.initials: YG
authors.lastname: Ermakova
authors.forename: D A
authors.initials: DA
authors.lastname: Sidorov-Biryukov
authors.forename: A B
authors.initials: AB
authors.lastname: Fedotov
authors.forename: P
authors.initials: P
authors.lastname: Hemmer
authors.forename: V V
authors.initials: VV
authors.lastname: Belousov
authors.forename: A M
authors.initials: AM
authors.lastname: Zheltikov
mesh_terms: D018130:Diamond; D004583:Electrons; D005336:Fiber Optic Technology / Q000379:methods*; D005453:Fluorescence; D007834:Lasers; D009474:Neurons; D009584:Nitrogen; D055100:Optical Fibers; D013696:Temperature; D062125:Thermometry / Q000379:methods*
chemical_list: D018130:Diamond; D009584:Nitrogen