title: Copper(II), cobalt(II) and nickel(II) complexes of lapachol: synthesis, DNA interaction, and cytotoxicity.
abstract: Three novel copper(II), cobalt(II), and nickel(II) complexes of lapachol (Lap) containing 110-phenanthroline (phen) ligand, [M(Lap)2(phen)] (M=Cu(II), 1, Co(II), 2, and Ni(II), 3), have been synthesized and characterized using, elemental analysis and spectroscopic studies. Their interactions with calf thymus DNA (CT DNA) were investigated using viscosity, thermal denaturation, circular dichorism, fluorescence quenching, and electronic absorption spectroscopy. The DNA cleavage abilities of 1-3 have been studied, where cleavage activity of copper complex 1 is more than the complexes 2 and 3. The in vitro cytotoxic potential of the complexes 1-3 against human cervical carcinoma (HeLa), human liver hepatocellular carcinoma (HepG-2), and human colorectal adenocarcinoma (HT-29) cells indicated their promising antitumor activity with quite low IC50values in the range of .15-2.41 μM, which are lower than those of cisplatin.
authors.affiliation: a Department of Chemistry , Isfahan University of Technology , Isfahan 84156-8311 , Iran.
authors.forename: Leila
authors.initials: L
authors.lastname: Tabrizi
authors.affiliation: a Department of Chemistry , Isfahan University of Technology , Isfahan 84156-8311 , Iran.
authors.forename: Faranak
authors.initials: F
authors.lastname: Talaie
authors.affiliation: a Department of Chemistry , Isfahan University of Technology , Isfahan 84156-8311 , Iran.
authors.forename: Hossein
authors.initials: H
authors.lastname: Chiniforoshan
mesh_terms: D000970:Antineoplastic Agents / Q000138:chemical synthesis* / Q000494:pharmacology; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D003035:Cobalt / Q000737:chemistry; D056831:Coordination Complexes / Q000138:chemical synthesis* / Q000494:pharmacology; D003300:Copper / Q000737:chemistry; D053837:DNA Cleavage; D004354:Drug Screening Assays, Antitumor; D006801:Humans; D020128:Inhibitory Concentration 50; D009285:Naphthoquinones / Q000737:chemistry; D009532:Nickel / Q000737:chemistry; D010957:Plasmids / Q000737:chemistry
chemical_list: D000970:Antineoplastic Agents; D056831:Coordination Complexes; D009285:Naphthoquinones; D003035:Cobalt; D003300:Copper; D009532:Nickel; C008252:lapachol
keywords: DNA binding; cytotoxicity; lapachol; metal complex