title: Tuberculosis Caused by Mycobacterium orygis in Dairy Cattle and Captured Monkeys in Bangladesh: a New Scenario of Tuberculosis in South Asia.
abstract: Mycobacterium orygis, commonly known as the oryx bacillus and a newly proposed Mycobacterium tuberculosis complex subspecies, was isolated from 18 cattle in a dairy farm and two captured rhesus monkeys in a zoo in Bangladesh. All the infected animals had tuberculosis lesions in their lungs, suggesting transmission and infection with M. orygis by an airborne route. The 20 isolates were analysed using a range of conventional and molecular typing methods, and RD-deletion typing and sequencing of selected genes confirmed the isolates as M. orygis. Multiple-locus variable-number tandem repeat analysis (MLVA) allowed the isolates to be divided into three clusters based on the relatedness of their MLVA profiles. The two monkey isolates shared the same MLVA pattern with 15 of the cattle isolates, whereas the remaining three cattle isolates had different patterns, even though the latter animals had been kept in the same dairy farm. The diversity observed among isolates may suggest the bacteria have been established in this area for a long period. This study along with other recent findings that report the detection of M. orygis from animals as well as humans originating from South Asia potentially indicate endemic distribution of M. orygis in South Asia.
authors.affiliation: Tuberculosis Laboratory, International Centre for Diarrhoeal Disease Research, Bangladesh, Mohakhali, Dhaka, Bangladesh.
authors.forename: Z
authors.initials: Z
authors.lastname: Rahim
authors.affiliation: Division of Bioresources, Hokkaido University Research Center for Zoonosis Control, Sapporo, Hokkaido, Japan.
authors.forename: J
authors.initials: J
authors.lastname: Thapa
authors.affiliation: Division of Bioresources, Hokkaido University Research Center for Zoonosis Control, Sapporo, Hokkaido, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Fukushima
authors.affiliation: Laboratory for Medical Microbiology and Public Health, Enschede, The Netherlands.
authors.forename: A G M
authors.initials: AGM
authors.lastname: van der Zanden
authors.affiliation: UCD School of Veterinary Medicine, University College Dublin, Dublin, Ireland.
authors.forename: S V
authors.initials: SV
authors.lastname: Gordon
authors.affiliation: Division of Bioresources, Hokkaido University Research Center for Zoonosis Control, Sapporo, Hokkaido, Japan.|The Global Station for Zoonosis Control, Hokkaido University Global Institution for Collaborative Research and Education, Sapporo, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Suzuki
authors.affiliation: Division of Bioresources, Hokkaido University Research Center for Zoonosis Control, Sapporo, Hokkaido, Japan.|The Global Station for Zoonosis Control, Hokkaido University Global Institution for Collaborative Research and Education, Sapporo, Japan.
authors.forename: C
authors.initials: C
authors.lastname: Nakajima
mesh_terms: D000818:Animals; D001459:Bangladesh; D002417:Cattle; D003612:Dairying; D005260:Female; D008253:Macaca mulatta; D058889:Molecular Typing / Q000662:veterinary; D008992:Monkey Diseases / Q000382:microbiology*; D009161:Mycobacterium / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D010802:Phylogeny; D014376:Tuberculosis / Q000382:microbiology / Q000662:veterinary*; D014380:Tuberculosis, Bovine / Q000382:microbiology
keywords: 
  Mycobacterium orygis
  ; Bangladesh; cattle; multilocus variable number of tandem repeat analysis; rhesus monkeys; tuberculosis