title: Actinocrinis puniceicyclus gen. nov., sp. nov., an actinobacterium isolated from an acidic spring.
abstract: An aerobic, mildly acidophilic actinobacterium was isolated from the Ochre Beds bog in Kootenay National Park, Canada. Cells of isolate OB1T were Gram-stain-positive, non-motile, pink- to purple-pigmented filaments. The pH range for growth was pH 3.5-6.5 (optimum pH 5.5), and the temperature range was 13-30°C. The major cellular fatty acids were i-C16 : 0 (28.5 %), i-C15 : 0 (14.6 %) and ai-C15 : 0 (14.3 %), and the major polar lipid was phosphohexose. The major quinone was menaquinone-11 (MK-11), and the peptidoglycan type was A1γ. The DNA G+C content was 70.2 %. Along with growth on complex media including yeast extract, proteose peptone, casamino acids and tryptic soy broth, growth occured on mono- and disaccharides (glucose, sucrose, galactose and xylose) and polysaccharides (starch, gellan, pectin, xylan and alginate). Anaerobic growth was not observed. The cells did not fix atmospheric nitrogen. On the basis of comparative 16S rRNA gene sequence analysis, this isolate belonged to the family Actinospicaceae, in the suborder Catenulisporineae of the order Actinomycetales. The most closely related species was Actinospica robiniae. However, the 16S rRNA gene sequence identity to this bacterium was low (92.8 %) and there were several chemotaxonomic differences from this species. We therefore propose a novel genus and species, Actinocrinis puniceicyclus gen. nov., sp. nov., with strain OB1T (=DSM 45618T=ATCC BAA-2771T) as the type strain.
authors.affiliation: Department of Biological Sciences, University of Calgary, Calgary, Alberta, T2N 1N4, Canada.
authors.forename: Joong-Jae
authors.initials: JJ
authors.lastname: Kim
authors.affiliation: Department of Biological Sciences, University of Calgary, Calgary, Alberta, T2N 1N4, Canada.
authors.forename: Colbran E
authors.initials: CE
authors.lastname: Marjerrison
authors.affiliation: Department of Biological Sciences, University of Calgary, Calgary, Alberta, T2N 1N4, Canada.
authors.forename: Sabrina L
authors.initials: SL
authors.lastname: Cornish Shartau
authors.affiliation: Department of Biological Sciences, University of Calgary, Calgary, Alberta, T2N 1N4, Canada.
authors.forename: Allyson L
authors.initials: AL
authors.lastname: Brady
authors.affiliation: Department of Biological Sciences, University of Calgary, Calgary, Alberta, T2N 1N4, Canada.
authors.forename: Christine E
authors.initials: CE
authors.lastname: Sharp
authors.affiliation: Department of Marine Microbiology and Biogeochemistry, NIOZ Royal Netherlands Institute for Sea Research, and Utrecht University, PO Box 59, 1790 AB Den Burg, The Netherlands.
authors.forename: W Irene C
authors.initials: WIC
authors.lastname: Rijpstra
authors.affiliation: Department of Marine Microbiology and Biogeochemistry, NIOZ Royal Netherlands Institute for Sea Research, and Utrecht University, PO Box 59, 1790 AB Den Burg, The Netherlands.|Faculty of Geosciences, Utrecht University, P.O. Box 80.021, 3508 TA Utrecht, The Netherlands.
authors.forename: Jaap S
authors.initials: JS
authors.lastname: Sinninghe Damsté
authors.affiliation: The Leibniz-Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, Braunschweig, Germany.
authors.forename: Peter
authors.initials: P
authors.lastname: Schumann
authors.affiliation: Geological Survey of Canada, Natural Resources Canada, Calgary, AB T2L 2A7, Canada.
authors.forename: Stephen E
authors.initials: SE
authors.lastname: Grasby
authors.affiliation: Department of Biological Sciences, University of Calgary, Calgary, Alberta, T2N 1N4, Canada.
authors.forename: Peter F
authors.initials: PF
authors.lastname: Dunfield
mesh_terms: D000143:Acids; D039903:Actinobacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D015373:Bacterial Typing Techniques; D001482:Base Composition; D002170:Canada; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D060985:Natural Springs / Q000382:microbiology*; D010457:Peptidoglycan / Q000737:chemistry; D010802:Phylogeny; D010858:Pigmentation; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D024482:Vitamin K 2 / Q000737:chemistry
chemical_list: D000143:Acids; D004269:DNA, Bacterial; D005227:Fatty Acids; D010457:Peptidoglycan; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2