title: A simple approach to spectrally resolved fluorescence and bright field microscopy over select regions of interest.
abstract: A standard wide field inverted microscope was converted to a spatially selective spectrally resolved microscope through the addition of a polarizing beam splitter, a pair of polarizers, an amplitude-mode liquid crystal-spatial light modulator, and a USB spectrometer. The instrument is capable of simultaneously imaging and acquiring spectra over user defined regions of interest. The microscope can also be operated in a bright-field mode to acquire absorption spectra of micron scale objects. The utility of the instrument is demonstrated on three different samples. First, the instrument is used to resolve three differently labeled fluorescent beads in vitro. Second, the instrument is used to recover time dependent bleaching dynamics that have distinct spectral changes in the cyanobacteria, Synechococcus leopoliensis UTEX 625. Lastly, the technique is used to acquire the absorption spectra of CH3NH3PbBr3perovskites and measure differences between nanocrystal films and micron scale crystals.
authors.affiliation: Graduate Program in the Biophysical Sciences, Institute for Biophysical Dynamics, and The James Franck Institute, The University of Chicago, Chicago, Illinois 60637, USA.
authors.forename: Peter D
authors.initials: PD
authors.lastname: Dahlberg
authors.affiliation: Graduate Program in the Biophysical Sciences, Institute for Biophysical Dynamics, and The James Franck Institute, The University of Chicago, Chicago, Illinois 60637, USA.
authors.forename: Christopher T
authors.initials: CT
authors.lastname: Boughter
authors.affiliation: Graduate Program in the Biophysical Sciences, Institute for Biophysical Dynamics, and The James Franck Institute, The University of Chicago, Chicago, Illinois 60637, USA.
authors.forename: Nabil F
authors.initials: NF
authors.lastname: Faruk
authors.affiliation: Graduate Program in the Biophysical Sciences, Institute for Biophysical Dynamics, and The James Franck Institute, The University of Chicago, Chicago, Illinois 60637, USA.
authors.forename: Lu
authors.initials: L
authors.lastname: Hong
authors.affiliation: Graduate Program in the Biophysical Sciences, Institute for Biophysical Dynamics, and The James Franck Institute, The University of Chicago, Chicago, Illinois 60637, USA.
authors.forename: Young Hoon
authors.initials: YH
authors.lastname: Koh
authors.affiliation: Graduate Program in the Biophysical Sciences, Institute for Biophysical Dynamics, and The James Franck Institute, The University of Chicago, Chicago, Illinois 60637, USA.
authors.forename: Matthew A
authors.initials: MA
authors.lastname: Reyer
authors.affiliation: Graduate Program in the Biophysical Sciences, Institute for Biophysical Dynamics, and The James Franck Institute, The University of Chicago, Chicago, Illinois 60637, USA.
authors.forename: Alon
authors.initials: A
authors.lastname: Shaiber
authors.affiliation: Graduate Program in the Biophysical Sciences, Institute for Biophysical Dynamics, and The James Franck Institute, The University of Chicago, Chicago, Illinois 60637, USA.
authors.forename: Aiman
authors.initials: A
authors.lastname: Sherani
authors.affiliation: Graduate Program in the Biophysical Sciences, Institute for Biophysical Dynamics, and The James Franck Institute, The University of Chicago, Chicago, Illinois 60637, USA.
authors.forename: Jiacheng
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Graduate Program in the Biophysical Sciences, Institute for Biophysical Dynamics, and The James Franck Institute, The University of Chicago, Chicago, Illinois 60637, USA.
authors.forename: Justin E
authors.initials: JE
authors.lastname: Jureller
authors.affiliation: Graduate Program in the Biophysical Sciences, Institute for Biophysical Dynamics, and The James Franck Institute, The University of Chicago, Chicago, Illinois 60637, USA.
authors.forename: Adam T
authors.initials: AT
authors.lastname: Hammond