title: Differential Expression of Newly Identified Long Intergenic Non-Coding RNAs in Buffalo Oocytes Indicating Their Possible Role in Maturation and Embryonic Development.
abstract: Female germ cell and its intricate milieu regulate key processes of folliculogenesis and early embryonic development. However, the composition and dynamics of the oocyte transcriptome defines its future fertilizing ability which in turn depends on a number of oocyte specific genes whose identities are still unknown. In this context, the construction of buffalo oocyte specific subtracted cDNA library has raised fresh challenges of defining the importance of a battery of oocyte expressed transcripts in oocyte maturation. The present study tried to characterize these hitherto unknown transcripts and further to assess their expression dynamics in buffalo oocytes of different quality. For this purpose, three ESTs were selected from the library and subjected to 5' and 3' RACE for generating their full length sequences. These constructed full length sequences were validated by amplifying them in oocytes. Further these sequences were extensively analyzed for their coding potential and possible role using coding potential calculator and miRNA database. Besides, their expression was monitored during in vitro maturation in good (BCB+) and poor quality (BCB-) oocytes which was interestingly found to be differing significantly. All the three sequences under study were interpreted as long intergenic non-coding RNAs with the possibility of two of them acting as a miRNA precursors. Also, their differential expression trends in competitively diverse oocytes hints at their possible involvement in oocyte maturation and future embryonic development which needs to be explored further. J. Cell. Biochem. 118: 1712-1721, 2017. © 2016 Wiley Periodicals, Inc.
authors.affiliation: Animal Biotechnology Centre, National Dairy Research Institute, Karnal, Haryana, India.
authors.forename: Sunny
authors.initials: S
authors.lastname: Dholpuria
authors.affiliation: Animal Biotechnology Centre, National Dairy Research Institute, Karnal, Haryana, India.
authors.forename: Manish
authors.initials: M
authors.lastname: Kumar
authors.affiliation: Animal Biotechnology Centre, National Dairy Research Institute, Karnal, Haryana, India.
authors.forename: Sandeep
authors.initials: S
authors.lastname: Kumar
authors.affiliation: Animal Biotechnology Centre, National Dairy Research Institute, Karnal, Haryana, India.
authors.forename: Parul
authors.initials: P
authors.lastname: Sarwalia
authors.affiliation: Department of Animal Science, Michigan State University, East Lansing, Michigan.
authors.forename: Sandeep
authors.initials: S
authors.lastname: Rajput
authors.affiliation: Animal Biotechnology Centre, National Dairy Research Institute, Karnal, Haryana, India.
authors.forename: Rakesh
authors.initials: R
authors.lastname: Kumar
authors.affiliation: Animal Biotechnology Centre, National Dairy Research Institute, Karnal, Haryana, India.
authors.forename: Sachinandan
authors.initials: S
authors.lastname: De
authors.affiliation: Animal Biotechnology Centre, National Dairy Research Institute, Karnal, Haryana, India.
authors.forename: Tirtha K
authors.initials: TK
authors.lastname: Datta
mesh_terms: D000818:Animals; D002020:Buffaloes / Q000196:embryology* / Q000235:genetics*; D019295:Computational Biology; D047108:Embryonic Development / Q000235:genetics / Q000502:physiology; D005260:Female; D015723:Gene Library; D005854:Germ Cells / Q000166:cytology / Q000378:metabolism; D009865:Oocytes / Q000166:cytology / Q000378:metabolism; D062085:RNA, Long Noncoding / Q000235:genetics*
chemical_list: D062085:RNA, Long Noncoding
keywords: BUFFALO FERTILITY; NON-CODING RNA; OOCYTE COMPETENCE