title: Ultraslow Domain Motions in HIV-1 TAR RNA Revealed by Solid-State Deuterium NMR.
abstract: Intrinsic motions may allow HIV-1 transactivation response (TAR) RNA to change its conformation to form a functional complex with the Tat protein, which is essential for viral replication. Understanding the dynamic properties of TAR necessitates determining motion on the intermediate nanosecond-to-microsecond time scale. To this end, we performed solid-state deuterium NMR line-shape and T1Zrelaxation-time experiments to measure intermediate motions for two uridine residues, U40 and U42, within the lower helix of TAR. We infer global motions at rates of ∼105s-1in the lower helix, which are much slower than those in the upper helix (∼106s-1), indicating that the two helical domains reorient independently of one another in the solid-state sample. These results contribute to the aim of fully describing the properties of functional motions in TAR RNA.
authors.affiliation: Department of Chemistry, University of Washington , Box 351700, Seattle 98195, United States.
authors.forename: Wei
authors.initials: W
authors.lastname: Huang
authors.affiliation: Department of Chemistry, University of Washington , Box 351700, Seattle 98195, United States.
authors.forename: Prashant S
authors.identifier: 0000-0002-3091-5231
authors.initials: PS
authors.lastname: Emani
authors.affiliation: Department of Chemistry, University of Washington , Box 351700, Seattle 98195, United States.
authors.forename: Gabriele
authors.initials: G
authors.lastname: Varani
authors.affiliation: Department of Chemistry, University of Washington , Box 351700, Seattle 98195, United States.
authors.forename: Gary P
authors.identifier: 0000-0002-7293-1897
authors.initials: GP
authors.lastname: Drobny
mesh_terms: D003903:Deuterium; D016325:HIV Long Terminal Repeat; D009682:Magnetic Resonance Spectroscopy; D012367:RNA, Viral / Q000737:chemistry*
chemical_list: D012367:RNA, Viral; D003903:Deuterium