title: Simultaneous determination of 35 ultra-trace level organophosphorus pesticide residues in Sanjie Zhentong capsules of traditional Chinese medicine using ultra high performance liquid chromatography with tandem mass spectrometry.
abstract: The aim of this study was to develop an analytical method for the simultaneous determination of ultra-trace levels of 35 organophosphorus pesticide residues in Sanjie Zhentong capsules, a traditional Chinese medicine prescription. A method based on multiclass and multiresidue sample preparation was developed. First, samples were hydrated with water at 4°C. A ratio of 1:3 sample/water was used for each of the sample amounts. Then, different extraction solvents were screened. This step was followed by a dispersive solid-phase extraction clean-up procedure using both primary secondary amine and polyamide. A comprehensive sensitive multiresidue liquid chromatography tandem mass spectrometry method was investigated and validated. Good linearity was achieved in the range of 10-500 μg/kg for each analyte. The average recovery ranged from 70 to 120%, except for methamidophos, fonophos, diazinon, and chlorpyriphos-ethyl, the recoveries of which ranged from 60-70% at the lower concentration level. The precision values were lower than 10% for all the compounds in three concentration levels. The limits of detection and limits of quantification values were 0.01-2.1 and 0.05-3.4 μg/kg, respectively. The matrix effects were determined, and most of the compounds showed signal suppression. Finally, this optimized procedure was applied for the analysis of real samples.
authors.affiliation: School of Pharmacy, China Pharmaceutical University, Nanjing, China.|State Key Lab of New-tech for Chinese Medicine Pharmaceutical Process, Lianyungang, China.
authors.forename: Jiachun
authors.initials: J
authors.lastname: Li
authors.affiliation: State Key Lab of New-tech for Chinese Medicine Pharmaceutical Process, Lianyungang, China.|Kanion Pharmaceutical Co., Ltd., Lianyungang, China.
authors.forename: Dongpo
authors.initials: D
authors.lastname: Li
authors.affiliation: State Key Lab of New-tech for Chinese Medicine Pharmaceutical Process, Lianyungang, China.
authors.forename: Jingling
authors.initials: J
authors.lastname: Wu
authors.affiliation: State Key Lab of New-tech for Chinese Medicine Pharmaceutical Process, Lianyungang, China.
authors.forename: Jianping
authors.initials: J
authors.lastname: Qin
authors.affiliation: State Key Lab of New-tech for Chinese Medicine Pharmaceutical Process, Lianyungang, China.
authors.forename: Junhua
authors.initials: J
authors.lastname: Hu
authors.affiliation: State Key Lab of New-tech for Chinese Medicine Pharmaceutical Process, Lianyungang, China.|Kanion Pharmaceutical Co., Ltd., Lianyungang, China.
authors.forename: Wenzhe
authors.initials: W
authors.lastname: Huang
authors.affiliation: State Key Lab of New-tech for Chinese Medicine Pharmaceutical Process, Lianyungang, China.|Kanion Pharmaceutical Co., Ltd., Lianyungang, China.
authors.forename: Zhenzhong
authors.initials: Z
authors.lastname: Wang
authors.affiliation: State Key Lab of New-tech for Chinese Medicine Pharmaceutical Process, Lianyungang, China.|Kanion Pharmaceutical Co., Ltd., Lianyungang, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Xiao
authors.affiliation: School of Pharmacy, China Pharmaceutical University, Nanjing, China.|Jiangsu Institute for Food and Drug Control, Nanjing, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Wang
mesh_terms: D002214:Capsules; D002851:Chromatography, High Pressure Liquid; D004340:Drug Contamination; D004365:Drugs, Chinese Herbal / Q000032:analysis*; D008516:Medicine, Chinese Traditional; D009943:Organophosphorus Compounds / Q000032:analysis*; D010573:Pesticide Residues / Q000032:analysis*; D052616:Solid Phase Extraction; D053719:Tandem Mass Spectrometry
chemical_list: D002214:Capsules; D004365:Drugs, Chinese Herbal; D009943:Organophosphorus Compounds; D010573:Pesticide Residues
keywords: Sanjie Zhentong capsules; dispersive solid-phase extraction; pesticides; polyamide; traditional Chinese medicine