title: Overexpression of SOX18 promotes prostate cancer progression via the regulation of TCF1, c-Myc, cyclin D1 and MMP-7.
abstract: Sex determining region Y (SRY)-box 18 (SOX18) gene encodes transcription factors that have been recently confirmed to be overexpressed in various human types of cancer and maintain the malignant behavior of cancer cells. However, the role and its potential function in prostate cancer (PCa) has not been demonstrated and the mechanisms of SOX18 involved in tumor progression remain largely unclear. In the present study, the expression of SOX18 was analyzed in 98 PCa and 81 adjacent non-tumor tissues using immunohistochemistry. The data showed that SOX18 was overexpressed in 72 of 98 (73.5%) PCa tissues compared with that in 28 of 81 (34.6%) non-tumor tissues. In addition, the expression of SOX18 was related with the clinical features of patients with PCa. To explore the potential role of SOX18 in PCa cells, Cell Counting Kit-8 (CCK-8), migration, invasion and xenograft assays were performed. Our data showed that knockdown of SOX18 decreased the proliferation, migration and invasion of PCa cells in vitro, in addition to the tumor growth in vivo. Markedly, SOX18 knockdown caused the decreased expression of TCF1, c-Myc, cyclin D1 and MMP-7. In conclusion, SOX18 was overexpressed in PCa and may regulate the malignant capacity of cells via the upregulation of TCF1, c-Myc, cyclin D1 and MMP-7.
authors.affiliation: Department of Urology, Peking University People's Hospital, Beijing, P.R. China.
authors.forename: Huaqi
authors.initials: H
authors.lastname: Yin
authors.affiliation: Department of Urology, Peking University People's Hospital, Beijing, P.R. China.
authors.forename: Zhengzuo
authors.initials: Z
authors.lastname: Sheng
authors.affiliation: Department of Urology, Peking University People's Hospital, Beijing, P.R. China.
authors.forename: Xiaowei
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Department of Urology, Peking University People's Hospital, Beijing, P.R. China.
authors.forename: Yiqing
authors.initials: Y
authors.lastname: Du
authors.affiliation: Department of Urology, Peking University International Hospital, Beijing, P.R. China.
authors.forename: Caipeng
authors.initials: C
authors.lastname: Qin
authors.affiliation: Department of Urology, Peking University People's Hospital, Beijing, P.R. China.
authors.forename: Huixin
authors.initials: H
authors.lastname: Liu
authors.affiliation: Department of Urology, Peking University People's Hospital, Beijing, P.R. China.
authors.forename: Yaojun
authors.initials: Y
authors.lastname: Dun
authors.affiliation: Department of Urology, Peking University People's Hospital, Beijing, P.R. China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Wang
authors.affiliation: Department of Urology, Peking University People's Hospital, Beijing, P.R. China.
authors.forename: Chengyue
authors.initials: C
authors.lastname: Jin
authors.affiliation: Department of Urology, Central Hospital of Qingdao City, Qingdao, P.R. China.
authors.forename: Yanhui
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: Department of Urology, Peking University People's Hospital, Beijing, P.R. China.
authors.forename: Tao
authors.initials: T
authors.lastname: Xu
mesh_terms: D000368:Aged; D000818:Animals; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D049109:Cell Proliferation / Q000235:genetics; D019938:Cyclin D1 / Q000378:metabolism; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D055785:Gene Knockdown Techniques; D051538:Hepatocyte Nuclear Factor 1-alpha / Q000378:metabolism; D006801:Humans; D008297:Male; D020783:Matrix Metalloproteinase 7 / Q000378:metabolism; D008819:Mice, Nude; D011471:Prostatic Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology*; D016271:Proto-Oncogene Proteins c-myc / Q000378:metabolism; D055760:SOXF Transcription Factors / Q000235:genetics* / Q000378:metabolism; D023041:Xenograft Model Antitumor Assays
chemical_list: C530104:CCND1 protein, human; C496487:HNF1A protein, human; D051538:Hepatocyte Nuclear Factor 1-alpha; D016271:Proto-Oncogene Proteins c-myc; C095473:SOX18 protein, human; D055760:SOXF Transcription Factors; D019938:Cyclin D1; C060932:MMP7 protein, human; D020783:Matrix Metalloproteinase 7