title: Antibody repertoire profiling with mimotope arrays.
abstract: Large-scale profiling and monitoring of antibody repertoires is possible through next generation sequencing (NGS), phage display libraries and microarrays. These methods can be combined in a pipeline, which ultimately maps the antibody reactivities onto defined arrays of structures - peptides or carbohydrates. The arrays can help analyze the individual specificities or can be used as complex patterns. In any case, the targets recognized should formally be considered mimotopes unless they are proven to be epitopes driving the antibody synthesis. Here, the advantages and disadvantages of the major profiling techniques as well as their current and future application in disease prediction and vaccination are discussed.
authors.affiliation: a Institute of Biology and Immunology of Reproduction, Bulgarian Academy of Sciences , Sofia , Bulgaria.
authors.forename: Shina
authors.initials: S
authors.lastname: Pashova
authors.affiliation: b Institute of Pharmacology, University of Bern , Bern , Switzerland.
authors.forename: Christoph
authors.initials: C
authors.lastname: Schneider
authors.affiliation: b Institute of Pharmacology, University of Bern , Bern , Switzerland.
authors.forename: Stephan
authors.initials: S
authors.lastname: von Gunten
authors.affiliation: c Stephan Angeloff Institute of Microbiology, Bulgarian Academy of Sciences , Sofia , Bulgaria.
authors.forename: Anastas
authors.initials: A
authors.lastname: Pashov
mesh_terms: D000818:Animals; D000906:Antibodies / Q000097:blood*; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods*; D006801:Humans; D019151:Peptide Library; D040081:Protein Array Analysis / Q000379:methods*
chemical_list: D000906:Antibodies; D019151:Peptide Library
keywords: NGS; antibody repertoire; glycan; microarray; peptide; phage display