title: Organ-specific effects of low-dose zinc pre-exposure on high-dose zinc induced mitochondrial dysfunction in large yellow croaker Pseudosciaena crocea.
abstract: The study was carried out to evaluate the effects of low-dose zinc (Zn) pre-exposure on survival rate, new Zn accumulation, and mitochondrial bioenergetics in the liver and spleen of large yellow croaker exposed to high-dose Zn. To the end, fish were pre-exposed to 0 and 2 mg L-1Zn for 48 h and post-exposed to 0 and 12 mg L-1Zn for 48 h. Twelve milligrams Zn per liter exposure alone reduced survival rate, but the effect did not appear in the 2 mg L-1Zn pre-exposure groups. Two milligrams per liter Zn pre-exposure also ameliorated 12 mg Zn L-1induced new Zn accumulation, reactive oxygen species (ROS) levels, and mitochondrial swelling in the liver. However, these effects did not appear in the spleen. In the liver, 2 mg L-1Zn pre-exposure apparently relieved 12 mg L-1Zn induced down-regulation of activities of ATP synthase (F-ATPase), succinate dehydrogenase (SDH), and malate dehydrogenase (MDH). The mRNA levels of these genes remained relatively stable in fish exposed to 12 mg L-1Zn alone, but increased in fish exposed to 12 mg L-1Zn with 2 mg L-1Zn pre-treatment. In the spleen, 2 mg Zn L-1pre-exposure did not mitigate the down-regulation of mRNA levels of genes and activities of relative enzymes induced by 12 mg L-1Zn. In conclusion, our study demonstrated low-dose zinc pre-exposure ameliorated high-dose zinc induced mitochondrial dysfunction in the liver but not in the spleen of large yellow croaker, indicating an organ-specific effect.
authors.affiliation: National Engineering Research Center of Marine Facilities Aquaculture, Zhejiang Ocean University, Zhoushan, 316022, People's Republic of China.
authors.forename: Jia-Lang
authors.initials: JL
authors.lastname: Zheng
authors.affiliation: National Engineering Research Center of Marine Facilities Aquaculture, Zhejiang Ocean University, Zhoushan, 316022, People's Republic of China.
authors.forename: Shuang-Shuang
authors.initials: SS
authors.lastname: Yuan
authors.affiliation: National Engineering Research Center of Marine Facilities Aquaculture, Zhejiang Ocean University, Zhoushan, 316022, People's Republic of China.
authors.forename: Bin
authors.initials: B
authors.lastname: Shen
authors.affiliation: National Engineering Research Center of Marine Facilities Aquaculture, Zhejiang Ocean University, Zhoushan, 316022, People's Republic of China. wucw2013@126.com.
authors.forename: Chang-Wen
authors.initials: CW
authors.lastname: Wu
mesh_terms: D000818:Animals; D015536:Down-Regulation; D029941:Fish Proteins / Q000378:metabolism; D005786:Gene Expression Regulation / Q000187:drug effects; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008291:Malate Dehydrogenase; D008928:Mitochondria / Q000187:drug effects* / Q000378:metabolism; D025261:Mitochondrial Proton-Translocating ATPases / Q000378:metabolism; D010473:Perciformes / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism; D013154:Spleen / Q000187:drug effects / Q000378:metabolism; D013385:Succinate Dehydrogenase / Q000378:metabolism; D015032:Zinc / Q000493:pharmacokinetics / Q000494:pharmacology*
chemical_list: D029941:Fish Proteins; D012333:RNA, Messenger; D017382:Reactive Oxygen Species; D008291:Malate Dehydrogenase; D013385:Succinate Dehydrogenase; D025261:Mitochondrial Proton-Translocating ATPases; D015032:Zinc
keywords: Large yellow croaker; Metal acclimation; Mitochondrial energy metabolism; Zinc