title: Preparation, physicochemical characterization and antioxidant activity of diphenyl diselenide-loaded poly(lactic acid) nanoparticles.
abstract: In this study, we developed, characterized and evaluated the antioxidant activity of poly (lactic acid) nanoparticles containing diphenyl diselenide (PhSe)2. Nanoparticles were characterized in terms of mean particle size, polydispersity index, zeta potential, encapsulation efficiency, in vitro release profile, physical stability, polymer-drug interactions and thermal properties. Also, the antioxidant activity of nanoparticles on hypochlorous acid (HOCl) was assessed. Nanoparticles presented a mean size of 210nm, had low polydispersity, zeta potential of -24mV, and an encapsulation efficiency over 90%. Differential scanning calorimetry and X-ray diffraction results showed (PhSe)2is dispersed in PLA matrix in an amorphous state. Lyophilized nanoparticles maintained physical stability over three months, while nanoparticles dispersed in water did not present stability over 7days. In vitro release assay was characterized by a biphasic release pattern with burst effect in 8h followed by a sustained release diffusion governed over 192h. Nanoencapsulation did not alter the antioxidant activity of (PhSe)2on HOCl. The study concludes these properties of (PhSe)2-loaded nanoparticles can be useful to extend the biological effects of (PhSe)2.
authors.affiliation: Laboratory of Pharmaceutical Nanotechnology, Department of Pharmacy, Universidade Estadual do Centro-Oeste/UNICENTRO, Guarapuava/PR, Brazil.
authors.forename: Osmar
authors.initials: O
authors.lastname: Dos Reis Antunes Junior
authors.affiliation: Laboratory of Pharmaceutical Nanotechnology, Department of Pharmacy, Universidade Estadual do Centro-Oeste/UNICENTRO, Guarapuava/PR, Brazil.
authors.forename: Emilli
authors.initials: E
authors.lastname: Antônio
authors.affiliation: Laboratory of Pharmaceutical Nanotechnology, Department of Pharmacy, Universidade Estadual do Centro-Oeste/UNICENTRO, Guarapuava/PR, Brazil.
authors.forename: Rubiana Mara
authors.initials: RM
authors.lastname: Mainardes
authors.affiliation: Laboratory of Pharmaceutical Nanotechnology, Department of Pharmacy, Universidade Estadual do Centro-Oeste/UNICENTRO, Guarapuava/PR, Brazil. Electronic address: najehunicentro@hotmail.com.
authors.forename: Najeh Maissar
authors.initials: NM
authors.lastname: Khalil
mesh_terms: D000975:Antioxidants / Q000737:chemistry*; D001555:Benzene Derivatives / Q000737:chemistry*; D002627:Chemistry, Physical; D053758:Nanoparticles / Q000737:chemistry*; D016566:Organoselenium Compounds / Q000737:chemistry*; D011091:Polyesters / Q000737:chemistry*
chemical_list: D000975:Antioxidants; D001555:Benzene Derivatives; D016566:Organoselenium Compounds; D011091:Polyesters; C061132:diphenyldiselenide; C033616:poly(lactide)
keywords: Antioxidant; Diphenyl diselenide; Drug release; Polymeric nanoparticles