title: Oral Epithelial Cell Culture Model for Studying the Pathogenesis of Chronic Inflammatory Disease.
abstract: The interactions between bacteria, epithelium, and neutrophilic polymorphonuclear leukocytes (neutrophils) are the key to the initiation and progression of many chronic inflammatory-immune diseases. In addition, all can be influenced by external factors, such as micronutrients, thereby providing potentially novel approaches to therapy. This chapter will therefore provide detailed methods for core techniques involved in studying cellular and molecular epithelial responses to a bacterial challenge in relation to chronic inflammatory disease pathogenesis and therapy.
authors.affiliation: Institute of Clinical Sciences, College of Medical and Dental Sciences, The School of Dentistry, University of Birmingham, 5 Mill Pool Way, Edgbaston, Birmingham, B5 7EG, UK.
authors.forename: Mike R
authors.initials: MR
authors.lastname: Milward
authors.affiliation: Institute of Clinical Sciences, College of Medical and Dental Sciences, The School of Dentistry, University of Birmingham, 5 Mill Pool Way, Edgbaston, Birmingham, B5 7EG, UK.
authors.forename: Martin R
authors.initials: MR
authors.lastname: Ling
authors.affiliation: Institute of Clinical Sciences, College of Medical and Dental Sciences, The School of Dentistry, University of Birmingham, 5 Mill Pool Way, Edgbaston, Birmingham, B5 7EG, UK.
authors.forename: Melissa M
authors.initials: MM
authors.lastname: Grant
authors.affiliation: Institute of Clinical Sciences, College of Medical and Dental Sciences, The School of Dentistry, University of Birmingham, 5 Mill Pool Way, Edgbaston, Birmingham, B5 7EG, UK. i.l.c.chapple@bham.ac.uk.
authors.forename: Iain L C
authors.initials: IL
authors.lastname: Chapple
mesh_terms: D015415:Biomarkers; D018929:Cell Culture Techniques; D002908:Chronic Disease; D019295:Computational Biology / Q000379:methods; D004847:Epithelial Cells / Q000378:metabolism*; D015870:Gene Expression; D006801:Humans; D007150:Immunohistochemistry; D007249:Inflammation / Q000209:etiology* / Q000378:metabolism* / Q000473:pathology; D009061:Mouth Mucosa / Q000166:cytology* / Q000378:metabolism*; D016133:Polymerase Chain Reaction
chemical_list: D015415:Biomarkers
keywords: DNA; Electrophoresis; Epithelium; Gene expression; H400 oral epithelial cells; Immunocytochemistry; NF-kappa B; Polymerase chain reaction; RNA