title: In vitro effect of myo-inositol on sperm motility in normal and oligoasthenospermia patients undergoing in vitro fertilization.
abstract: It is a known fact that abnormal seminal liquid specimens contain abnormal amounts of oxygen free radicals and reactive oxygen species (ROS), and that the use of antioxidant molecules both in vivo and in vitro leads to improvement of semen quality in terms of motility, reduction in DNA damage, with obvious consequences on the fertilization potential. Myo-inositol has been observed to have anti-oxidant properties and be present in much greater concentrations specifically in seminal liquid than in the blood. Moreover, there seems to be a direct relationship between myo-inositol and mitochondrial membrane potential (MMP) and sperm motility. Studies performed in vivo have demonstrated that a dietary supplementation with myo-inositol in men undergoing assisted reproduction techniques may improve sperm quality and motility in oligoasthenospermia (OAT) patients. In the following study we utilized myo-inositol in vitro to verify its effect on semen quality in both normal and OAT patients undergoing in vitro fertilization (IVF) with respect to standard sperm medium. In vitro incubation of seminal liquid carried out using myo-inositol (Andrositol-Lab, Lo.Li. Pharma-Roma, Italy) at a concentration of 15 μl/ml improved progressive motility in both normospermia and OAT subjects. In our opinion, myo-inositol may prove to be a useful strategy to improve sperm preparation for clinical use in IVF.
authors.affiliation: a Department of Clinical and Experimental Medicine , Division of Obstetrics and Gynecology Oncology, University of Pisa , Pisa , Italy and.
authors.forename: P G
authors.initials: PG
authors.lastname: Artini
authors.affiliation: a Department of Clinical and Experimental Medicine , Division of Obstetrics and Gynecology Oncology, University of Pisa , Pisa , Italy and.
authors.forename: E
authors.initials: E
authors.lastname: Casarosa
authors.affiliation: a Department of Clinical and Experimental Medicine , Division of Obstetrics and Gynecology Oncology, University of Pisa , Pisa , Italy and.
authors.forename: E
authors.initials: E
authors.lastname: Carletti
authors.affiliation: b Division of Obstetrics and Gynecology , Ospedale San Francesco , Barga , Lucca , Italy.
authors.forename: P
authors.initials: P
authors.lastname: Monteleone
authors.affiliation: a Department of Clinical and Experimental Medicine , Division of Obstetrics and Gynecology Oncology, University of Pisa , Pisa , Italy and.
authors.forename: A
authors.initials: A
authors.lastname: Di Noia
authors.affiliation: a Department of Clinical and Experimental Medicine , Division of Obstetrics and Gynecology Oncology, University of Pisa , Pisa , Italy and.
authors.forename: O M
authors.initials: OM
authors.lastname: Di Berardino
mesh_terms: D000328:Adult; D053627:Asthenozoospermia / Q000188:drug therapy*; D005307:Fertilization in Vitro / Q000379:methods*; D006801:Humans; D007294:Inositol / Q000494:pharmacology*; D008297:Male; D009845:Oligospermia / Q000188:drug therapy*; D013081:Sperm Motility / Q000187:drug effects*; D014803:Vitamin B Complex / Q000494:pharmacology*
chemical_list: D014803:Vitamin B Complex; D007294:Inositol
keywords: IVF; myo-inositol; semen preparation; semen quality