title: Synthesis of copper-containing bioactive glass nanoparticles using a modified Stöber method for biomedical applications.
abstract: Copper (Cu)-containing bioactive glasses (BGs) are attracting attention for bone regeneration and wound healing since they have bone-bonding capability and potential osteogenesis and angiogenesis properties. In this study, highly dispersed and spherical Cu-containing bioactive glass nanoparticles (Cu-BGNs) were successfully synthesized via a modified Stöber method. The content of incorporated Cu in the particles could be tailored by adjusting the amount of the added Cu precursor, a procedure that had no significant effects on the morphological and structural characteristics of the nanoparticles. Cu-BGNs exhibited satisfactory apatite-forming ability, as a large quantity of apatite could form on Cu-BGNs pellets after immersion in simulated body fluid for just 3days. The incorporation of Cu exhibited positive effects on the apatite formation. In addition, both Si and Cu ions were released from the Cu-BGN in a sustained manner for at least 14days in cell culture medium, indicating the potential of the BGN as promising carriers for delivering therapeutic Cu ions. Moreover, Cu-BGNs showed no significant cytotoxicity towards human mesenchymal stem cells and fibroblast cells at concentrations of 100, 10 and 1μg/mL. Taken together, the results suggest that Cu-BGNs are promising nanoparticulate fillers to develop nanocomposites for biomedical applications especially in bone regeneration and wound healing.
authors.affiliation: Institute of Biomaterials, Department of Materials Science and Engineering, University of Erlangen-Nuremberg, Cauerstrasse 6, 91058 Erlangen, Germany.
authors.forename: Kai
authors.initials: K
authors.lastname: Zheng
authors.affiliation: Department of Surgery, Klinikum rechts der Isar, Technische Universitaet München, Ismaninger Str. 22, 81675 München, Germany.
authors.forename: Xinyi
authors.initials: X
authors.lastname: Dai
authors.affiliation: Department of Surgery, Klinikum rechts der Isar, Technische Universitaet München, Ismaninger Str. 22, 81675 München, Germany.
authors.forename: Miao
authors.initials: M
authors.lastname: Lu
authors.affiliation: Department of Surgery, Klinikum rechts der Isar, Technische Universitaet München, Ismaninger Str. 22, 81675 München, Germany.
authors.forename: Norbert
authors.initials: N
authors.lastname: Hüser
authors.affiliation: Lehrstuhl für Chemische Reaktionstechnik, University of Erlangen-Nuremberg, Egerlandstrasse 3, 91058 Erlangen, Germany.
authors.forename: Nicola
authors.initials: N
authors.lastname: Taccardi
authors.affiliation: Institute of Biomaterials, Department of Materials Science and Engineering, University of Erlangen-Nuremberg, Cauerstrasse 6, 91058 Erlangen, Germany. Electronic address: aldo.boccaccini@ww.uni-erlangen.de.
authors.forename: Aldo R
authors.initials: AR
authors.lastname: Boccaccini
mesh_terms: D001031:Apatites / Q000737:chemistry; D001672:Biocompatible Materials / Q000737:chemistry*; D001861:Bone Regeneration; D018929:Cell Culture Techniques / Q000379:methods; D003300:Copper / Q000737:chemistry*; D005347:Fibroblasts / Q000166:cytology; D005898:Glass / Q000737:chemistry*; D006801:Humans; D007477:Ions; D059630:Mesenchymal Stem Cells / Q000166:cytology; D053761:Nanocomposites / Q000737:chemistry; D053758:Nanoparticles / Q000737:chemistry*; D036103:Nanotechnology / Q000379:methods; D009389:Neovascularization, Pathologic; D010012:Osteogenesis; D010316:Particle Size; D017550:Spectroscopy, Fourier Transform Infrared; D014945:Wound Healing; D014961:X-Ray Diffraction
chemical_list: D001031:Apatites; D001672:Biocompatible Materials; D007477:Ions; D003300:Copper
keywords: Apatite formation; Bioactive glass nanoparticles; Copper; Ion release; Stöber method