title: The clinical significance and biological function of olfactomedin 4 in triple negative breast cancer.
abstract: Olfactomedin 4 abnormal expression has been observed in several types of human cancer, but the status of olfactomedin 4 in triple negative breast cancer is still unknown. The aim of our study is to explore the clinical significance and biological function of olfactomedin 4 in triple negative breast cancer. The mRNA and protein expression of olfactomedin 4 in triple negative breast cancer tissues and cell lines was detected, and the correlation between olfactomedin 4 expression and clinicopathological factors was analyzed by immunohistochemistry. The biological function of olfactomedin 4 on tumor-metastasis was explored by Transwell migration assay and invasion assay in vitro. In our results, olfactomedin 4 mRNA and protein expression is decreased in triple-negative breast cancer tissues and cell lines. Olfactomedin 4 protein low-expression associated with lymph node metastasis, distant metastasis, clinical stage and poor prognosis of triple-negative breast cancer patients. Up-regulation of olfactomedin 4 suppresseed triple-negative breast cancer cells migration and invasion, and reduced cell metastasis-associated protein MMP 9 expression. In conclusion, olfactomedin 4 is a novel biomarker of triple-negative breast cancer for predicting prognosis and developing targeted molecular therapies.
authors.affiliation: Surgery Teaching and Research Section, Clinical Medical School, Jining Medical University, No. 16 Hehua Road, Jining, Shandong 272067, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Xiong
authors.affiliation: Surgery Teaching and Research Section, Clinical Medical School, Jining Medical University, No. 16 Hehua Road, Jining, Shandong 272067, China.
authors.forename: Xuefeng
authors.initials: X
authors.lastname: Lei
authors.affiliation: Surgery Teaching and Research Section, Clinical Medical School, Jining Medical University, No. 16 Hehua Road, Jining, Shandong 272067, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Academy of Basic Medicine, Jining Medical University, No. 16 Hehua Road, Jining, Shandong 272067, China. Electronic address: fujia2000@hainan.com.
authors.forename: Jia
authors.initials: J
authors.lastname: Fu
mesh_terms: D014408:Biomarkers, Tumor / Q000096:biosynthesis* / Q000235:genetics; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000502:physiology; D005260:Female; D016179:Granulocyte Colony-Stimulating Factor / Q000096:biosynthesis* / Q000235:genetics; D006801:Humans; D064726:Triple Negative Breast Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology
chemical_list: D014408:Biomarkers, Tumor; C454127:OLFM4 protein, human; D016179:Granulocyte Colony-Stimulating Factor
keywords: Biomarker; Breast cancer; Metastasis; Olfactomedin 4; Prognosis