title: Detection of novel GII.17 norovirus in Argentina, 2015.
abstract: During the winter of 2014-2015 a novel GII.17 norovirus strain emerged as a cause of large gastroenteritis outbreaks in Asia; displacing the long-term predominant strain, GII.4. Although sporadically detected, the emerging GII.17 virus was described in North America and Europe. In this study, we describe the presence of this novel strain in Argentina (South America), and provide new information on the genetic diversity of GII.17 noroviruses. Ten stool samples from individuals (1-88years old; median: 5years old) experiencing gastroenteritis symptoms from San Martín de los Andes, Argentina were tested for Norovirus using RT-PCR. Subsequently, Norovirus positive samples were analyzed by sequencing. Norovirus was found in four out of 10 samples received. Partial sequencing of the ORF2 was available for 3/4 samples: two samples belonged to genotype GII.4 and one to genotype GII.17 (Arg13099). Sequence analyses of the VP1 encoding region revealed that the GII.17 Argentinean strain presented characteristics from both, the new (cluster C), and older (cluster A and B) GII.17 strains. Phylogenetic and sequence analyses of the RdRp region showed that this strain was closely related to strains from genotypes GII.P3, GII.P13 and GII.P17; however, did not cluster within any of them. This study represents the first report of this emergent strain in South America, and presents further evidence of the genetic plasticity of the GII.17.
authors.affiliation: Instituto Nacional de Enfermedades Infecciosas (ANLIS "Dr. Carlos G. Malbrán"), Buenos Aires, Argentina. Electronic address: jdegiuseppe@anlis.gov.ar.
authors.forename: Juan I
authors.initials: JI
authors.lastname: Degiuseppe
authors.affiliation: Instituto Nacional de Enfermedades Infecciosas (ANLIS "Dr. Carlos G. Malbrán"), Buenos Aires, Argentina.
authors.forename: Karina A
authors.initials: KA
authors.lastname: Gomes
authors.affiliation: Zona Sanitaria IV, Neuquén, Argentina.
authors.forename: María F
authors.initials: MF
authors.lastname: Hadad
authors.affiliation: Division of Viral Products, Food and Drug Administration, Silver Spring, MD, USA.
authors.forename: Gabriel I
authors.initials: GI
authors.lastname: Parra
authors.affiliation: Instituto Nacional de Enfermedades Infecciosas (ANLIS "Dr. Carlos G. Malbrán"), Buenos Aires, Argentina.
authors.forename: Juan A
authors.initials: JA
authors.lastname: Stupka
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001118:Argentina; D017250:Caliciviridae Infections / Q000821:virology*; D036022:Capsid Proteins / Q000235:genetics; D002648:Child; D002675:Child, Preschool; D005243:Feces / Q000821:virology; D005759:Gastroenteritis / Q000821:virology*; D005838:Genotype; D006801:Humans; D007223:Infant; D008875:Middle Aged; D029322:Norovirus / Q000145:classification* / Q000235:genetics*; D010802:Phylogeny; D055815:Young Adult
chemical_list: D036022:Capsid Proteins
keywords: Argentina; GII.17; Gastroenteritis; Norovirus