title: Site-selective installation of BASHY fluorescent dyes to Annexin V for targeted detection of apoptotic cells.
abstract: Fluorophores are indispensable for imaging biological processes. We report the design and synthesis of azide-tagged boronic acid salicylidenehydrazone (BASHY) dyes and their use for site-selective labelling of Annexin V. The Annexin V-BASHY conjugate maintained function and fluorescence as demonstrated by the targeted detection of apoptotic cells.
authors.affiliation: Instituto de Medicina Molecular, Faculdade de Medicina, Universidade de Lisboa, Avenida Professor Egas Moniz, 1649-028, Lisboa, Portugal. gbernardes@medicina.ulisboa.pt and Department of Chemistry, University of Cambridge, Lensfield Road, CB2 1EW Cambridge, UK. gb453@cam.ac.uk.
authors.forename: Pedro M S D
authors.initials: PM
authors.lastname: Cal
authors.affiliation: Instituto de Medicina Molecular, Faculdade de Medicina, Universidade de Lisboa, Avenida Professor Egas Moniz, 1649-028, Lisboa, Portugal. gbernardes@medicina.ulisboa.pt.
authors.forename: Florian
authors.initials: F
authors.lastname: Sieglitz
authors.affiliation: Research Institute for Medicines (iMed.ULisboa), Faculty of Pharmacy, Universidade de Lisboa, Av. Prof. Gama Pinto, 1649-003, Lisboa, Portugal. pedrogois@ff.ul.pt.
authors.forename: Fábio M F
authors.initials: FM
authors.lastname: Santos
authors.affiliation: CIQSO - Center for Research in Sustainable Chemistry and Department of Chemistry, University of Huelva, Campus de El Carmen s/n, 21071 Huelva, Spain.
authors.forename: Cátia
authors.initials: C
authors.lastname: Parente Carvalho
authors.affiliation: Instituto de Medicina Molecular, Faculdade de Medicina, Universidade de Lisboa, Avenida Professor Egas Moniz, 1649-028, Lisboa, Portugal. gbernardes@medicina.ulisboa.pt.
authors.forename: Ana
authors.initials: A
authors.lastname: Guerreiro
authors.affiliation: Department of Chemistry, University of Cambridge, Lensfield Road, CB2 1EW Cambridge, UK. gb453@cam.ac.uk.
authors.forename: Jean B
authors.initials: JB
authors.lastname: Bertoldo
authors.affiliation: CIQSO - Center for Research in Sustainable Chemistry and Department of Chemistry, University of Huelva, Campus de El Carmen s/n, 21071 Huelva, Spain.
authors.forename: Uwe
authors.initials: U
authors.lastname: Pischel
authors.affiliation: Research Institute for Medicines (iMed.ULisboa), Faculty of Pharmacy, Universidade de Lisboa, Av. Prof. Gama Pinto, 1649-003, Lisboa, Portugal. pedrogois@ff.ul.pt.
authors.forename: Pedro M P
authors.initials: PM
authors.lastname: Gois
authors.affiliation: Instituto de Medicina Molecular, Faculdade de Medicina, Universidade de Lisboa, Avenida Professor Egas Moniz, 1649-028, Lisboa, Portugal. gbernardes@medicina.ulisboa.pt and Department of Chemistry, University of Cambridge, Lensfield Road, CB2 1EW Cambridge, UK. gb453@cam.ac.uk.
authors.forename: Gonçalo J L
authors.identifier: 0000-0001-6594-8917
authors.initials: GJ
authors.lastname: Bernardes
mesh_terms: D017304:Annexin A5 / Q000737:chemistry*; D017209:Apoptosis; D001897:Boronic Acids / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D006835:Hydrazones / Q000737:chemistry*; D018613:Microscopy, Confocal; D008958:Models, Molecular; D008968:Molecular Conformation
chemical_list: D017304:Annexin A5; D001897:Boronic Acids; D005456:Fluorescent Dyes; D006835:Hydrazones