title: Gene Delivery for the Generation of Bioartificial Pacemaker.
abstract: Electronic pacemakers have been used in patients with heart rhythm disorders for device-supported pacing. While effective, there are such shortcomings as limited battery life, permanent implantation of catheters, the lack of autonomic neurohumoral responses, and risks of lead dislodging. Here we describe protocols for establishing porcine models of sick sinus syndrome and complete heart block, and the generation of bioartificial pacemaker by delivering a strategically engineered form of hyperpolarization-activated cyclic nucleotide-gated pacemaker channel protein via somatic gene transfer to convert atrial or ventricular muscle cardiomyocytes into nodal-like cells that rhythmically fire action potentials.
authors.affiliation: Stem Cell & Regenerative Medicine Consortium, LKS Faculty of Medicine, The University of Hong Kong, Pokfulam, Hong Kong.
authors.forename: Patrick K W
authors.initials: PK
authors.lastname: Chan
authors.affiliation: Dr. Li Dak-Sum Research Center for Regenerative Medicine, The University of Hong Kong, 5/F Hong Kong Jockey Club Building for Interdisciplinary Research, 5 Sassoon Road, Pokfulam, Hong Kong. ronaldli@hku.hk.|Icahn School of Medicine at Mount Sinai, Manhattan, NY, USA. ronaldli@hku.hk.|Ming-Wai Lau Center for Reparative Medicine, Karolinska Institutet, Stockholm, Sweden. ronaldli@hku.hk.
authors.forename: Ronald A
authors.initials: RA
authors.lastname: Li
mesh_terms: D000818:Animals; D000229:Dependovirus / Q000378:metabolism; D005471:Fluoroscopy; D018014:Gene Transfer Techniques; D049452:Green Fluorescent Proteins / Q000378:metabolism; D057809:HEK293 Cells; D006801:Humans; D010138:Pacemaker, Artificial; D034421:Sus scrofa
chemical_list: D049452:Green Fluorescent Proteins
keywords: Adeno-associated virus; Adenovirus; Bioartificial pacemaker; Complete heart block; Gene transfer; Heart rhythms; Hyperpolarization-activated cyclic nucleotide-gated (HCN) channel; Sick sinus syndrome