title: Aloe vera (Aloe barbadensis Miller) supplemented probiotic lassi prevents Shigella infiltration from epithelial barrier into systemic blood flow in mice model.
abstract: The aim of present work was to investigate preventive role of orally administered Aloe vera supplemented probiotic lassi (APL) on Shigella dysenteriae infection in mice. At the end of experimental period (2, 5 and 7 days of challenging), different organs such as spleen, liver, small intestine, large intestine, and peritoneal fluid were collected and assessed for Shigella colonization. Secretary IgA was estimated in intestinal fluid. Blood was collected in heparinized tubes for various haematological studies. Oral administration of APL showed a significant (p < 0.05) reduction in the Shigella counts (log cfu/mL) in all organs as compared to other treatment groups at different intervals after post feeding. Similarly, secretary IgA antibody levels (μg/mL) in intestinal fluid were significantly (p < 0.05) increased in case of APL fed mice. Further, feeding of APL also demonstrated a positive effect on different haematological parameters viz. Hb (gm %), RBC and WBC count. The results indicated the immunoprotective effects of APL against Shigella dysenteriae induced infection in mice.
authors.affiliation: Dairy Technology Division, ICAR-National Dairy Research Institute, Karnal 132001, Haryana, India. Electronic address: abdulndri@gmail.com.
authors.forename: Shaik Abdul
authors.initials: SA
authors.lastname: Hussain
authors.affiliation: Dairy Technology Division, ICAR-National Dairy Research Institute, Karnal 132001, Haryana, India.
authors.forename: Girdhari Ramdas
authors.initials: GR
authors.lastname: Patil
authors.affiliation: Animal Biochemistry Division, ICAR-National Dairy Research Institute, Karnal 132001, Haryana, India.
authors.forename: Srinu
authors.initials: S
authors.lastname: Reddi
authors.affiliation: Dairy Technology Division, ICAR-National Dairy Research Institute, Karnal 132001, Haryana, India.
authors.forename: Vidhu
authors.initials: V
authors.lastname: Yadav
authors.affiliation: Animal Biochemistry Division, ICAR-National Dairy Research Institute, Karnal 132001, Haryana, India.
authors.forename: Ramesh
authors.initials: R
authors.lastname: Pothuraju
authors.affiliation: Dairy Technology Division, ICAR-National Dairy Research Institute, Karnal 132001, Haryana, India.
authors.forename: Ram Ran Bijoy
authors.initials: RRB
authors.lastname: Singh
authors.affiliation: Animal Biochemistry Division, ICAR-National Dairy Research Institute, Karnal 132001, Haryana, India.
authors.forename: Suman
authors.initials: S
authors.lastname: Kapila
mesh_terms: D000504:Aloe / Q000737:chemistry; D000818:Animals; D000898:Antibiosis; D016470:Bacteremia / Q000188:drug therapy / Q000276:immunology / Q000382:microbiology* / Q000517:prevention & control; D058491:Bacterial Load; D019587:Dietary Supplements; D004195:Disease Models, Animal; D004405:Dysentery, Bacillary / Q000178:diet therapy / Q000276:immunology / Q000382:microbiology*; D007071:Immunoglobulin A, Secretory / Q000276:immunology; D007413:Intestinal Mucosa / Q000276:immunology / Q000382:microbiology*; D051379:Mice; D010936:Plant Extracts / Q000276:immunology; D019936:Probiotics; D012760:Shigella / Q000472:pathogenicity*
chemical_list: D007071:Immunoglobulin A, Secretory; D010936:Plant Extracts
keywords: Aloe vera; Immunomodulatory; Lassi; Pathogenesis; Probiotics; Shigella