title: Investigation of thiol-disulphide balance in patients with acute urticaria and chronic spontaneous urticaria.
abstract: BACKGROUND
  Thiol-disulphide balance plays a major role in health and diseases. This balance may be disrupted by various diseases. We aimed to determine status of the effect of thiol-disulphide balance in urticaria.
  
  
  OBJECTIVE
  We aimed to investigate the thiol-disulphide balance in patients with acute urticaria (AUP) and chronic spontaneous urticaria (CSU).
  
  
  METHODS
  Study included 53 AUP and 47 healthy controls plus 57 patients with chronic spontaneous urticaria (CSUP) and 57 healthy controls. Levels of native thiols, disulphides and total thiols were evaluated in plasma using a new and automated spectrophotometric method. Ratios of disulphides/total thiols, disulphides/native thiols and native thiols/total thiols were calculated.
  
  
  RESULTS
  For AU, there was no statistical difference compared to control group in levels of native thiols, disulphides and total thiols. For CSU, however, there was an increase in levels of native thiols, disulphides and total thiols and the ratio of thiol/disulphide in favour of disulphide.
  
  
  CONCLUSIONS
  Thiol-disulphide balance was not affected by AU but shifted towards to disulphide in CSU indicating the presence of oxidative stress (OS).
authors.affiliation: a Department of Dermatology , Ministry of Health Atatürk Training and Research Hospital , Ankara , Turkey.
authors.forename: Ayse
authors.initials: A
authors.lastname: Akbas
authors.affiliation: a Department of Dermatology , Ministry of Health Atatürk Training and Research Hospital , Ankara , Turkey.
authors.forename: Fadime
authors.initials: F
authors.lastname: Kilinc
authors.affiliation: a Department of Dermatology , Ministry of Health Atatürk Training and Research Hospital , Ankara , Turkey.
authors.forename: Sertac
authors.initials: S
authors.lastname: Sener
authors.affiliation: b Department of Dermatology , School of Medicine, Yıldırım Beyazıt University , Ankara , Turkey and.
authors.forename: Akın
authors.initials: A
authors.lastname: Aktaş
authors.affiliation: c Department of Biochemistry , Ministry of Health Atatürk Training and Research Hospital , Ankara , Turkey.
authors.forename: Pervin
authors.initials: P
authors.lastname: Baran
authors.affiliation: c Department of Biochemistry , Ministry of Health Atatürk Training and Research Hospital , Ankara , Turkey.
authors.forename: Merve
authors.initials: M
authors.lastname: Ergin
mesh_terms: D000208:Acute Disease; D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D016022:Case-Control Studies; D002908:Chronic Disease; D004220:Disulfides / Q000097:blood*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D013438:Sulfhydryl Compounds / Q000097:blood*; D014581:Urticaria / Q000097:blood* / Q000453:epidemiology; D055815:Young Adult
chemical_list: D004220:Disulfides; D013438:Sulfhydryl Compounds
keywords: Acute urticaria; chronic spontaneous urticaria; oxidative stress; thiol–disulphide balance