title: Premature chromatid separation and altered proliferation of human leukocytes treated with vanadium (III) oxide.
abstract: Vanadium is a widely distributed metal in the Earth's surface and is released into the environment by either natural or anthropogenic causes. Vanadium (III) oxide (V2O3) is present in the environment, and many organisms are exposed to this compound; however, its effects at the cellular and genetic levels are still unknown. Therefore, in this study, the ability of V2O3to induce chromosomal damage and impair cell proliferation was tested on human leukocytes in vitro. The cultures cells were treated for 48 h with different concentrations 2, 4, 8 or 16 μg/mL of V2O3, and we use the sister chromatid exchange's (SCE) test and the viability assay to evaluate the effects. In the results, no change was observed in either the viability or the frequency of SCE; however, a significant increase was observed in the incidence of premature chromatid separation (PCS), and a decrease was observed in both the mitotic index (MI) and the replication index (RI). Therefore, it can be suggested that V2O3induces a genotoxic effect at the centromere level, indicating that it is a cause of aneuploidy that is capable of altering cell cycle progression.
authors.affiliation: a Facultad de Estudios Superiores-Zaragoza , UNAM, Unidad de Investigación en Genética y Toxicología Ambiental (UNIGEN) Laboratorio 5-PA Unidad Multidisciplinaria de Investigación Experimental (UMIE-Z) , Ciudad de México , México and.|b Posgrado en Ciencias Biológicas, UNAM, Edificio E, Primer Piso, Circuito de Posgrados, Ciudad Universitaria Del. Coyoacán , Ciudad de México , México.
authors.forename: Rodrigo Anibal
authors.initials: RA
authors.lastname: Mateos-Nava
authors.affiliation: a Facultad de Estudios Superiores-Zaragoza , UNAM, Unidad de Investigación en Genética y Toxicología Ambiental (UNIGEN) Laboratorio 5-PA Unidad Multidisciplinaria de Investigación Experimental (UMIE-Z) , Ciudad de México , México and.
authors.forename: Juan José
authors.initials: JJ
authors.lastname: Rodríguez-Mercado
authors.affiliation: a Facultad de Estudios Superiores-Zaragoza , UNAM, Unidad de Investigación en Genética y Toxicología Ambiental (UNIGEN) Laboratorio 5-PA Unidad Multidisciplinaria de Investigación Experimental (UMIE-Z) , Ciudad de México , México and.
authors.forename: Mario Agustín
authors.initials: MA
authors.lastname: Altamirano-Lozano
mesh_terms: D000328:Adult; D024422:Aneugens / Q000633:toxicity; D002274:Carcinogens, Environmental / Q000633:toxicity*; D002453:Cell Cycle / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D002503:Centromere / Q000187:drug effects* / Q000378:metabolism; D002842:Chromatids / Q000187:drug effects* / Q000378:metabolism; D004261:DNA Replication / Q000187:drug effects*; D006801:Humans; D007962:Leukocytes / Q000166:cytology / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism; D008297:Male; D008940:Mitotic Index; D009152:Mutagenicity Tests; D009994:Osmolar Concentration; D010087:Oxides / Q000633:toxicity*; D012854:Sister Chromatid Exchange / Q000187:drug effects; D017968:Vanadium Compounds / Q000633:toxicity*; D055815:Young Adult
chemical_list: D024422:Aneugens; D002274:Carcinogens, Environmental; D010087:Oxides; D017968:Vanadium Compounds
keywords: Vanadium (III) oxide; citostatic; lymphocytes; premature chromatid separation; sister chromatid exchange