title: Biohydrogen production from enzymatic hydrolysis of food waste in batch and continuous systems.
abstract: In this study, the feasibility of biohydrogen production from enzymatic hydrolysis of food waste was investigated. Food waste (solid-to-liquid ratio of 10%, w/v) was first hydrolyzed by commercial glucoamylase to release glucose (24.35 g/L) in the food waste hydrolysate. Then, the obtained food waste hydrolysate was used as substrate for biohydrogen production in the batch and continuous (continuous stirred tank reactor, CSTR) systems. It was observed that the maximum cumulative hydrogen production of 5850 mL was achieved with a yield of 245.7 mL hydrogen/g glucose (1.97 mol hydrogen/mol glucose) in the batch system. In the continuous system, the effect of hydraulic retention time (HRT) on biohydrogen production from food waste hydrolysate was investigated. The optimal HRT obtained from this study was 6 h with the highest hydrogen production rate of 8.02 mmol/(h·L). Ethanol and acetate were the major soluble microbial products with low propionate production at all HRTs. Enzymatic hydrolysis of food waste could effectively accelerate hydrolysis speed, improve substrate utilization rate and increase hydrogen yield.
authors.affiliation: College of Materials and Environmental Engineering, Hangzhou Dianzi University, Hangzhou 310018, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Han
authors.affiliation: College of Materials and Environmental Engineering, Hangzhou Dianzi University, Hangzhou 310018, China.
authors.forename: Yingting
authors.initials: Y
authors.lastname: Yan
authors.affiliation: College of Materials and Environmental Engineering, Hangzhou Dianzi University, Hangzhou 310018, China.
authors.forename: Yiwen
authors.initials: Y
authors.lastname: Shi
authors.affiliation: College of Materials and Environmental Engineering, Hangzhou Dianzi University, Hangzhou 310018, China.
authors.forename: Jingjing
authors.initials: J
authors.lastname: Gu
authors.affiliation: College of Materials and Environmental Engineering, Hangzhou Dianzi University, Hangzhou 310018, China.
authors.forename: Junhong
authors.initials: J
authors.lastname: Tang
authors.affiliation: College of Materials and Environmental Engineering, Hangzhou Dianzi University, Hangzhou 310018, China.
authors.forename: Hongting
authors.initials: H
authors.lastname: Zhao
mesh_terms: D061249:Batch Cell Culture Techniques; D055162:Biocatalysis; D056804:Biofuels / Q000600:supply & distribution*; D019149:Bioreactors; D005285:Fermentation; D005502:Food; D005087:Glucan 1,4-alpha-Glucosidase / Q000737:chemistry*; D005947:Glucose / Q000378:metabolism; D006859:Hydrogen / Q000378:metabolism*; D006868:Hydrolysis; D007700:Kinetics; D014866:Waste Products / Q000032:analysis*
chemical_list: D056804:Biofuels; D014866:Waste Products; D006859:Hydrogen; D005087:Glucan 1,4-alpha-Glucosidase; D005947:Glucose