title: Draft genome of a South African strain of Pectobacterium carotovorum subsp. brasiliense.
abstract: The draft genome of Pectobacterium carotovorum subsp. brasiliense (Pcb) which causes blackleg of potato was submitted to the NCBI and released with reference number NZ_LGRF00000000.1. The estimated genome size based on the draft genome assembly is 4,820,279bp from 33 contigs ranging in length from 444 to 1,660,019 nucleotides. The genome annotation showed 4250 putative genes, 4114 CDS and 43 pseudo-genes. Three complete rRNA gene species were detected: nine 5S, one 16S and one 23S. Other partial rRNA gene fragments were also identified, nine 16S rRNA and three 23S rRNA. A total of 69 tRNA genes and one ncRNA gene were also annotated in this genome.
authors.affiliation: University of South Africa, Science Campus, Department of Agriculture and Animal Health, Florida, South Africa. Electronic address: ntushk@unisa.ac.za.
authors.forename: Khayalethu
authors.initials: K
authors.lastname: Ntushelo
authors.affiliation: Formerly, Agricultural Research Council - Biotechnology Platform, Onderstepoort, South Africa.
authors.forename: Joseph
authors.initials: J
authors.lastname: Mafofo
mesh_terms: D019295:Computational Biology / Q000379:methods; D016680:Genome, Bacterial; D023281:Genomics / Q000379:methods; D059014:High-Throughput Nucleotide Sequencing; D058977:Molecular Sequence Annotation; D016973:Pectobacterium carotovorum / Q000235:genetics*; D017422:Sequence Analysis, DNA
keywords: Genome sequencing; Illumina; Pectobacterium carotovorum subsp. brasiliensis