title: MOPS and coxsackievirus B3 stability.
abstract: Study of coxsackievirus B3 strain 28 (CVB3/28) stability using MOPS to improve buffering in the experimental medium revealed that MOPS (3-morpholinopropane-1-sulfonic acid) increased CVB3 stability and the effect was concentration dependent. Over the pH range 7.0-7.5, virus stability was affected by both pH and MOPS concentration. Computer-simulated molecular docking showed that MOPS can occupy the hydrophobic pocket in capsid protein VP1 where the sulfonic acid head group can form ionic and hydrogen bonds with Arg95 and Asn211 near the pocket opening. The effects of MOPS and hydrogen ion concentrations on the rate of virus decay were modeled by including corresponding parameters in a recent kinetic model. These results indicate that MOPS can directly associate with CVB3 and stabilize the virus, possibly by altering capsid conformational dynamics.
authors.affiliation: Department of Pathology and Microbiology University of Nebraska Medical Center, 986495 Nebraska Medical Center, Omaha, NE 68198-6495, USA. Electronic address: scarson@unmc.edu.
authors.forename: Steven D
authors.initials: SD
authors.lastname: Carson
authors.affiliation: Department of Medicine, The Pennsylvania State University College of Medicine, 500 University Drive, Hershey, PA 17033, USA. Electronic address: shafenstein@hmc.psu.edu.
authors.forename: Susan
authors.initials: S
authors.lastname: Hafenstein
authors.affiliation: Department of Medicine, The Pennsylvania State University College of Medicine, 500 University Drive, Hershey, PA 17033, USA. Electronic address: hyl5126@psu.edu.
authors.forename: Hyunwook
authors.initials: H
authors.lastname: Lee
mesh_terms: D036022:Capsid Proteins / Q000235:genetics / Q000378:metabolism; D029822:Enterovirus B, Human / Q000737:chemistry* / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism; D004769:Enterovirus Infections / Q000821:virology*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D062105:Molecular Docking Simulation; D009025:Morpholines / Q000494:pharmacology*
chemical_list: D036022:Capsid Proteins; D009025:Morpholines; C008550:morpholinopropane sulfonic acid
keywords: Coxsackievirus; MOPS; Stability; pH