title: Nanocomputed Tomography Imaging of Bacterial Alkaline Phosphatase Activity with an Iodinated Hydrogelator.
abstract: Alkaline phosphatase (ALP) is an important enzyme, but direct imaging of ALP activity with high spatiotemporal resolution remains challenging. In this work, we rationally designed an iodinated hydrogelator precursor Nap-Phe-Phe(I)-Tyr(H2PO3)-OH (1P) which self-assembles into nanofibers to form hydrogel under the catalysis of ALP. With this property of concentrating iodine atoms at the locations of ALP, 1P was successfully applied for direct nanocomputed tomography (nano-CT) imaging of ALP activity in bacteria for the first time. We envision that, on the basis of this pioneering work, new hydrogelators containing more iodine atoms (e.g., five iodine atoms in 1P) will be designed for better nano-CT imaging of ALP activity with higher CT contrast in the near future.
authors.affiliation: CAS Key Laboratory of Soft Matter Chemistry, Department of Chemistry, University of Science and Technology of China , 96 Jinzhai Road, Hefei, Anhui 230026, China.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Zheng
authors.affiliation: CAS Key Laboratory of Soft Matter Chemistry, Department of Chemistry, University of Science and Technology of China , 96 Jinzhai Road, Hefei, Anhui 230026, China.|Institute of Nuclear Physics and Chemistry, Chinese Academy of Engineering Physics , 64 Mianshan Road, Mianyang, Sichuan 621900, China.
authors.forename: Anming
authors.initials: A
authors.lastname: Tang
authors.affiliation: National Synchrotron Radiation Laboratory, University of Science and Technology of China , 42 Hezuohua South Road, Hefei, Anhui 230029, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Guan
authors.affiliation: National Synchrotron Radiation Laboratory, University of Science and Technology of China , 42 Hezuohua South Road, Hefei, Anhui 230029, China.
authors.forename: Liang
authors.initials: L
authors.lastname: Chen
authors.affiliation: Analysis Center, Nanjing Medical University , Nanjing, Jiangsu 210093, China.
authors.forename: Fuqiang
authors.initials: F
authors.lastname: Wang
authors.affiliation: CAS Key Laboratory of Soft Matter Chemistry, Department of Chemistry, University of Science and Technology of China , 96 Jinzhai Road, Hefei, Anhui 230026, China.
authors.forename: Peiyao
authors.initials: P
authors.lastname: Chen
authors.affiliation: CAS Key Laboratory of Soft Matter Chemistry, Department of Chemistry, University of Science and Technology of China , 96 Jinzhai Road, Hefei, Anhui 230026, China.|Institute of Nuclear Physics and Chemistry, Chinese Academy of Engineering Physics , 64 Mianshan Road, Mianyang, Sichuan 621900, China.
authors.forename: Weijuan
authors.initials: W
authors.lastname: Wang
authors.affiliation: CAS Key Laboratory of Soft Matter Chemistry, Department of Chemistry, University of Science and Technology of China , 96 Jinzhai Road, Hefei, Anhui 230026, China.
authors.forename: Yufeng
authors.initials: Y
authors.lastname: Luo
authors.affiliation: National Synchrotron Radiation Laboratory, University of Science and Technology of China , 42 Hezuohua South Road, Hefei, Anhui 230029, China.
authors.forename: Yangchao
authors.initials: Y
authors.lastname: Tian
authors.affiliation: CAS Key Laboratory of Soft Matter Chemistry, Department of Chemistry, University of Science and Technology of China , 96 Jinzhai Road, Hefei, Anhui 230026, China.
authors.forename: Gaolin
authors.identifier: 0000-0002-6159-9999
authors.initials: G
authors.lastname: Liang
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism*; D055162:Biocatalysis; D004926:Escherichia coli / Q000201:enzymology*; D029968:Escherichia coli Proteins / Q000378:metabolism*; D020100:Hydrogels / Q000737:chemistry*; D007455:Iodine / Q000737:chemistry; D057139:Nanofibers / Q000737:chemistry; D009281:Naphthalenes / Q000737:chemistry*; D010446:Peptide Fragments / Q000737:chemistry*; D055114:X-Ray Microtomography
chemical_list: D029968:Escherichia coli Proteins; D020100:Hydrogels; C000628025:Nap-Phe-Phe(I)-Tyr(H2PO3)-OH; D009281:Naphthalenes; D010446:Peptide Fragments; D007455:Iodine; D000469:Alkaline Phosphatase