title: A Competitive Bio-Barcode Amplification Immunoassay for Small Molecules Based on Nanoparticles.
abstract: A novel detection method of small molecules, competitive bio-barcode amplification immunoassay, was developed and described in this report. Through the gold nanoparticles (AuNPs) probe and magnetic nanoparticles (MNPs) probe we prepared, only one monoclonal antibody can be used to detect small molecules. The competitive bio-barcode amplification immunoassay overcomes the obstacle that the bio-barcode assay cannot be used in small molecular detection, as two antibodies are unable to combine to one small molecule due to its small molecular structure. The small molecular compounds, triazophos, were selected as targets for the competitive bio-barcode amplification immunoassay. The linear range of detection was from 0.04 ng mL-1to 10 ng mL-1, and the limit of detection (LOD) was 0.02 ng mL-1, which was 10-20 folds lower than ELISA (Enzyme Linked Immunosorbent Assay). A practical application of the proposed immunoassay was evaluated by detecting triazophos in real samples. The recovery rate ranged from 72.5% to 110.5%, and the RSD was less than 20%. These results were validated by GC-MS, which indicated that this convenient and sensitive method has great potential for small molecular in real samples.
authors.affiliation: Key Laboratory for Agro-Products Quality and Food Safety, Institute of Quality Standards &Testing Technology for Agro-Products, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.
authors.forename: Pengfei
authors.initials: P
authors.lastname: Du
authors.affiliation: Key Laboratory for Agro-Products Quality and Food Safety, Institute of Quality Standards &Testing Technology for Agro-Products, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.
authors.forename: Maojun
authors.initials: M
authors.lastname: Jin
authors.affiliation: Key Laboratory for Agro-Products Quality and Food Safety, Institute of Quality Standards &Testing Technology for Agro-Products, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.
authors.forename: Ge
authors.initials: G
authors.lastname: Chen
authors.affiliation: Key Laboratory for Agro-Products Quality and Food Safety, Institute of Quality Standards &Testing Technology for Agro-Products, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.
authors.forename: Chan
authors.initials: C
authors.lastname: Zhang
authors.affiliation: Key Laboratory for Agro-Products Quality and Food Safety, Institute of Quality Standards &Testing Technology for Agro-Products, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.
authors.forename: Zejun
authors.initials: Z
authors.lastname: Jiang
authors.affiliation: Key Laboratory for Agro-Products Quality and Food Safety, Institute of Quality Standards &Testing Technology for Agro-Products, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.
authors.forename: Yanxin
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Key Laboratory for Agro-Products Quality and Food Safety, Institute of Quality Standards &Testing Technology for Agro-Products, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.
authors.forename: Pan
authors.initials: P
authors.lastname: Zou
authors.affiliation: Key Laboratory for Agro-Products Quality and Food Safety, Institute of Quality Standards &Testing Technology for Agro-Products, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.
authors.forename: Yongxin
authors.initials: Y
authors.lastname: She
authors.affiliation: Key Laboratory for Agro-Products Quality and Food Safety, Institute of Quality Standards &Testing Technology for Agro-Products, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.
authors.forename: Fen
authors.initials: F
authors.lastname: Jin
authors.affiliation: Key Laboratory for Agro-Products Quality and Food Safety, Institute of Quality Standards &Testing Technology for Agro-Products, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.
authors.forename: Hua
authors.initials: H
authors.lastname: Shao
authors.affiliation: Key Laboratory for Agro-Products Quality and Food Safety, Institute of Quality Standards &Testing Technology for Agro-Products, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.
authors.forename: Shanshan
authors.initials: S
authors.lastname: Wang
authors.affiliation: Key Laboratory for Agro-Products Quality and Food Safety, Institute of Quality Standards &Testing Technology for Agro-Products, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.
authors.forename: Lufei
authors.initials: L
authors.lastname: Zheng
authors.affiliation: Key Laboratory for Agro-Products Quality and Food Safety, Institute of Quality Standards &Testing Technology for Agro-Products, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Wang
mesh_terms: D000911:Antibodies, Monoclonal / Q000737:chemistry; D006046:Gold / Q000737:chemistry; D007118:Immunoassay / Q000379:methods*; D057230:Limit of Detection; D008280:Magnetics / Q000379:methods; D053768:Metal Nanoparticles / Q000737:chemistry*
chemical_list: D000911:Antibodies, Monoclonal; D006046:Gold