title: Immunochromatographic detection of the heat-labile enterotoxin of enterotoxigenic Escherichia coli with cross-detection of cholera toxin.
abstract: Here, we report the development of an immunochromatographic test strip that can detect heat-labile enterotoxin (LT) produced by enterotoxigenic Escherichia coli. Five types of monoclonal antibody (mAb)-producing hybridomas were isolated: three mAbs were A subunit specific and two were B subunit specific. Four mAbs also cross-reacted with both LT proteins derived from swine and human E. coli strains, but only one mAb 57B9 additionally cross-reacted with cholera toxin. Thus, mAb 57B9 was used to form a gold colloid-conjugated antibody for the immunochromatographic test by combination with polyclonal anti-LT rabbit IgG. This test strip detected not only LT in the culture supernatant of LT gene-positive strains, but also cholera toxin in the culture supernatant of Vibrio cholerae. These results indicate that this test strip is suitable for the diagnosis of both enterotoxigenic E. coli and V. cholerae infection.
authors.affiliation: Department of Microbiology, Fujita Health University School of Medicine, 1-98 Dengakugakubo, Kutsukake-cho, Toyoake, Aichi 470-1192, Japan. Electronic address: arimitsu@fujita-hu.ac.jp.
authors.forename: Hideyuki
authors.initials: H
authors.lastname: Arimitsu
authors.affiliation: Department of Microbiology, Fujita Health University School of Medicine, 1-98 Dengakugakubo, Kutsukake-cho, Toyoake, Aichi 470-1192, Japan. Electronic address: ksasaki@fujita-hu.ac.jp.
authors.forename: Keiko
authors.initials: K
authors.lastname: Sasaki
authors.affiliation: Department of Microbiology, Fujita Health University School of Medicine, 1-98 Dengakugakubo, Kutsukake-cho, Toyoake, Aichi 470-1192, Japan. Electronic address: ttsuji@fujita-hu.ac.jp.
authors.forename: Takao
authors.initials: T
authors.lastname: Tsuji
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000737:chemistry; D002771:Cholera / Q000175:diagnosis; D002772:Cholera Toxin / Q000032:analysis*; D002846:Chromatography, Affinity; D003001:Cloning, Molecular; D003470:Culture Media / Q000737:chemistry; D054307:Enterotoxigenic Escherichia coli / Q000302:isolation & purification*; D004768:Enterotoxins / Q000032:analysis*; D004927:Escherichia coli Infections / Q000175:diagnosis; D006358:Hot Temperature; D006801:Humans; D007074:Immunoglobulin G / Q000737:chemistry; D012680:Sensitivity and Specificity; D013552:Swine; D014734:Vibrio cholerae / Q000302:isolation & purification*
chemical_list: D000911:Antibodies, Monoclonal; D003470:Culture Media; D004768:Enterotoxins; D007074:Immunoglobulin G; D002772:Cholera Toxin
keywords: Cholera toxin; Enterotoxigenic Escherichia coli; Heat-labile enterotoxin; Immunochromatography; Monoclonal antibody