title: Evaluation of capillary electrophoresis for the analysis of rooibos and honeybush tea phenolics.
abstract: Rooibos and honeybush are popular herbal teas produced from the shrubs of Aspalathus linearis and Cyclopia spp., respectively, which are indigenous to South Africa. Both herbal teas are rich in polyphenols and their consumption is associated with several health benefits, partly ascribed to their phenolic constituents. Quantification of phenolics in extracts and teas for quality control and research purposes is generally performed using HPLC, although dedicated and often species-specific methods are required. CE offers an attractive alternative to HPLC for the analysis of phenolics, with potential benefits in terms of efficiency, speed and operating costs. In this contribution, we report quantitative CZE methods for the analysis of the principal honeybush and rooibos phenolics. Optimal separation for honeybush and rooibos phenolics was achieved in 21 and 32 min, respectively, with good linearity and repeatability. Quantitative data for extracts of "unfermented" and "fermented" rooibos and two honeybush species were statistically comparable with those obtained by HPLC for the majority of compounds. The developed methods demonstrated their utility for the comparison of phenolic contents between different species and as a function of manufacturing processes, thus offering cost effective, although less sensitive and robust, alternatives to HPLC analysis.
authors.affiliation: Department of Chemistry and Polymer Science, Stellenbosch University, Matieland, South Africa.
authors.forename: William J
authors.initials: WJ
authors.lastname: Arries
authors.affiliation: Department of Chemistry and Polymer Science, Stellenbosch University, Matieland, South Africa.
authors.forename: Andreas G J
authors.initials: AG
authors.lastname: Tredoux
authors.affiliation: Post-Harvest and Wine Technology Division, Agricultural Research Council, Stellenbosch, South Africa.
authors.forename: Dalene
authors.initials: D
authors.lastname: de Beer
authors.affiliation: Post-Harvest and Wine Technology Division, Agricultural Research Council, Stellenbosch, South Africa.|Department of Food Science, Stellenbosch University, Matieland, South Africa.
authors.forename: Elizabeth
authors.initials: E
authors.lastname: Joubert
authors.affiliation: Department of Chemistry and Polymer Science, Stellenbosch University, Matieland, South Africa.
authors.forename: André
authors.initials: A
authors.lastname: de Villiers
mesh_terms: D036903:Aspalathus / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D019075:Electrophoresis, Capillary / Q000379:methods*; D007887:Fabaceae / Q000737:chemistry*; D005285:Fermentation; D063427:Food Quality; D006801:Humans; D010636:Phenols / Q000032:analysis*; D010936:Plant Extracts / Q000032:analysis; D013019:South Africa; D000069459:Teas, Herbal / Q000032:analysis*
chemical_list: D010636:Phenols; D010936:Plant Extracts; D000069459:Teas, Herbal
keywords: Capillary zone electrophoresis; Herbal tea; Honeybush; Phenolics; Rooibos