title: Bone marrow T-cell percentage: A novel prognostic indicator in acute myeloid leukemia.
abstract: Acute myeloid leukemia (AML) is an aggressive malignancy for which overall disease-free survival is less than 50%. Manipulation of the immune system is an interesting and promising therapy for AML patients. We aimed to characterize the immune system of AML patients, highlighting the clinical relevance of total bone marrow (BM) lymphocytes and subpopulations. Sixty-six new AML cases diagnosed according to WHO criteria from King Abdullah Medical City, KSA, from October 2012 to February 2015. Analysis of BM lymphocytes and subpopulations was done by flowcytometry. Significantly, high percentages of BM lymphocytes, T cells, and natural killer (NK) cells were detected in the group that achieved complete remission (P values = 0.004, <0.001, and <0.001, respectively). Overall survival (OS) was significantly prolonged in patients with high BM lymphocytes and T cells (P values = 0.047 and P 0.002, respectively). Multivariate analysis indicated that BM T-cell percentage and cytogenetics were independent prognostic factors predictive of OS (HR 4.7, P value = 0.011). BM T-cell percentage constitutes a novel host factor that can be used in combination with cytogenetics to better predict OS. Large-scale multicenter studies are recommended to clarify its role as a predictor of OS and leukemia-free survival.
authors.affiliation: Clinical Pathology Department, National Cancer Institute, Cairo University, 4 Fum Al-Khalig Square, P.O. Box: 56042, Cario, 21955, Egypt. manarismail4@yahoo.com.|Laboratory Medicine Department, Faculty of Applied Medical Science, Um Al Qura University, Makkah, Kingdom of Saudi Arabia. manarismail4@yahoo.com.
authors.forename: Manar M
authors.initials: MM
authors.lastname: Ismail
authors.affiliation: Clinical Pathology Department, National Cancer Institute, Cairo University, 4 Fum Al-Khalig Square, P.O. Box: 56042, Cario, 21955, Egypt.|Laboratory and Blood Bank Department, King Abdulah Medical City, Makkah, Kingdom of Saudi Arabia.
authors.forename: Nahla A B
authors.initials: NAB
authors.lastname: Abdulateef
mesh_terms: D000328:Adult; D000368:Aged; D001853:Bone Marrow / Q000473:pathology*; D018572:Disease-Free Survival; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D007694:Killer Cells, Natural / Q000473:pathology; D015470:Leukemia, Myeloid, Acute / Q000175:diagnosis / Q000276:immunology / Q000401:mortality / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D011237:Predictive Value of Tests; D011379:Prognosis; D012074:Remission Induction; D015996:Survival Rate; D013601:T-Lymphocytes / Q000473:pathology*
keywords: AML; BM lymphocytes; Natural killer cells; OS; Prognosis; T-cells