title: Peroxisome proliferator-activated receptor-β/δ modulates mast cell phenotype.
abstract: The peroxisome proliferator-activated receptor-β/δ (PPARβ/δ) is known to have multiple anti-inflammatory effects, typically observed in endothelial cells, macrophages, T cells and B cells. Despite the fact that mast cells are important mediators of inflammation, to date, the role of PPARβ/δ in mast cells has not been examined. Hence, the present study examined the hypothesis that PPARβ/δ modulates mast cell phenotype. Bone-marrow-derived mast cells (BMMCs) and peritoneal mast cells from Pparβ/δ+/+mice expressed higher levels of high-affinity IgE receptor (FcεRI) compared with Pparβ/δ-/-mice. BMMCs from Pparβ/δ+/+mice also exhibited dense granules, associated with higher expression of enzymes and proteases compared with Pparβ/δ-/-mice. Resting BMMCs from Pparβ/δ+/+mice secreted lower levels of inflammatory cytokines, associated with the altered activation of phospholipase Cγ1 and extracellular signal-regulated kinases compared with Pparβ/δ-/-mice. Moreover, the production of cytokines by mast cells induced by various stimuli was highly dependent on PPARβ/δ expression. This study demonstrates that PPARβ/δ is an important regulator of mast cell phenotype.
authors.affiliation: Department of Veterinary and Biomedical Sciences, The Center of Molecular Toxicology and Carcinogenesis, The Pennsylvania State University, University Park, PA, USA.
authors.forename: Pei-Li
authors.initials: PL
authors.lastname: Yao
authors.affiliation: Department of Veterinary and Biomedical Sciences, The Center of Molecular Toxicology and Carcinogenesis, The Pennsylvania State University, University Park, PA, USA.
authors.forename: Jose L
authors.initials: JL
authors.lastname: Morales
authors.affiliation: Laboratory of Metabolism, National Cancer Institute, Bethesda, MD, USA.
authors.forename: Frank J
authors.initials: FJ
authors.lastname: Gonzalez
authors.affiliation: Department of Veterinary and Biomedical Sciences, The Center of Molecular Toxicology and Carcinogenesis, The Pennsylvania State University, University Park, PA, USA.
authors.forename: Jeffrey M
authors.initials: JM
authors.lastname: Peters
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000502:physiology*; D002454:Cell Differentiation; D002478:Cells, Cultured; D016207:Cytokines / Q000378:metabolism; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism; D005260:Female; D018836:Inflammation Mediators / Q000378:metabolism; D008407:Mast Cells / Q000502:physiology*; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D047494:PPAR delta / Q000235:genetics / Q000378:metabolism*; D048068:PPAR-beta / Q000235:genetics / Q000378:metabolism*; D010641:Phenotype; D017455:Receptors, IgE / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction / Q000235:genetics
chemical_list: D016207:Cytokines; D018836:Inflammation Mediators; D047494:PPAR delta; D048068:PPAR-beta; D017455:Receptors, IgE; D048049:Extracellular Signal-Regulated MAP Kinases
keywords: bone marrow-derived mast cells; cytokine; inflammation; peroxisome proliferator-activated receptor-β/δ