title: Efficient in vitro propagation ofArtemisia nilagiricavar.nilagirica(Indian wormwood) and assessment of genetic fidelity of micropropagated plants.
abstract: A reliable protocol has been established for in vitro propagation ofArtemisia nilagiricavar.nilagirica(Indian wormwood), a valuable medicinal plant from India. A highly proliferating organogenic callus was obtained on Murashige and Skoog (MS) medium supplemented with 2.5 µM IAA when nodal explants were cultured on MS medium supplemented with various growth regulators. Further, highest regeneration frequency (83.3 %) of adventitious shoots was observed, when the callus was sub-cultured on MS medium supplemented with 6-benzylaminopurine (BAP; 2.5 µM) along with 7.5 µM 2-isopentenyl adenine (2-iP). An optimal of 10.16 ± 2.24 shoots were regenerated on medium supplemented with 2.5 µM BAP + 7.5 µM 2-iP. Quarter strength MS medium supplemented with 10 µM IBA was effective for rooting of the shoots. Ex-vitro plants were normal and were established successfully. Cytological and molecular marker studies showed that regenerated plants showed genetic stability in micro-propagated plants.
authors.affiliation: Department of Botany, Karnatak University, Dharwad, 580003 India.
authors.forename: Smita
authors.initials: S
authors.lastname: Shinde
authors.affiliation: Department of Botany, Karnatak University, Dharwad, 580003 India.
authors.forename: Joseph Kadanthottu
authors.initials: JK
authors.lastname: Sebastian
authors.affiliation: Department of Biotechnology, Jain University, Bengaluru, 560069 India.
authors.forename: Jyothi Ramesh
authors.initials: JR
authors.lastname: Jain
authors.affiliation: Department of Botany, Karnatak University, Dharwad, 580003 India ; Department of Biotechnology, Jain University, Bengaluru, 560069 India.
authors.forename: Manohar Shirugumbi
authors.initials: MS
authors.lastname: Hanamanthagouda
authors.affiliation: Department of Botany, Karnatak University, Dharwad, 580003 India.
authors.forename: Hosakatte Niranjana
authors.initials: HN
authors.lastname: Murthy
keywords: Artemisia nilagirica; Genetic fidelity; Indian wormwood; Medicinal plant; Micropropagation; Multiple shoot regeneration