title: Potentiometric Aptasensing of Vibrio alginolyticus Based on DNA Nanostructure-Modified Magnetic Beads.
abstract: A potentiometric aptasensing assay that couples the DNA nanostructure-modified magnetic beads with a solid-contact polycation-sensitive membrane electrode for the detection ofVibrio alginolyticusis herein described. The DNA nanostructure-modified magnetic beads are used for amplification of the potential response and elimination of the interfering effect from a complex sample matrix. The solid-contact polycation-sensitive membrane electrode using protamine as an indicator is employed to chronopotentiometrically detect the change in the charge or DNA concentration on the magnetic beads, which is induced by the interaction betweenVibrio alginolyticusand the aptamer on the DNA nanostructures. The present potentiometric aptasensing method shows a linear range of 10-100 CFU mL-1with a detection limit of 10 CFU mL-1, and a good specificity for the detection ofVibrio alginolyticus. This proposed strategy can be used for the detection of other microorganisms by changing the aptamers in the DNA nanostructures.
authors.affiliation: Key Laboratory of Coastal Environmental Processes and Ecological Remediation, Yantai Institute of Coastal Zone Research (YIC), Chinese Academy of Sciences (CAS); Shandong Provincial Key Laboratory of Coastal Environmental Processes, YICCAS, Yantai 264003, Shandong, China. gtzhao@yic.ac.cn.|University of Chinese Academy of Sciences, Beijing 100049, China. gtzhao@yic.ac.cn.
authors.forename: Guangtao
authors.initials: G
authors.lastname: Zhao
authors.affiliation: Key Laboratory of Coastal Environmental Processes and Ecological Remediation, Yantai Institute of Coastal Zone Research (YIC), Chinese Academy of Sciences (CAS); Shandong Provincial Key Laboratory of Coastal Environmental Processes, YICCAS, Yantai 264003, Shandong, China. jwding@yic.ac.cn.
authors.forename: Jiawang
authors.initials: J
authors.lastname: Ding
authors.affiliation: Key Laboratory of Coastal Environmental Processes and Ecological Remediation, Yantai Institute of Coastal Zone Research (YIC), Chinese Academy of Sciences (CAS); Shandong Provincial Key Laboratory of Coastal Environmental Processes, YICCAS, Yantai 264003, Shandong, China. hyu@yic.ac.cn.|University of Chinese Academy of Sciences, Beijing 100049, China. hyu@yic.ac.cn.
authors.forename: Han
authors.initials: H
authors.lastname: Yu
authors.affiliation: Key Laboratory of Coastal Environmental Processes and Ecological Remediation, Yantai Institute of Coastal Zone Research (YIC), Chinese Academy of Sciences (CAS); Shandong Provincial Key Laboratory of Coastal Environmental Processes, YICCAS, Yantai 264003, Shandong, China. tjyin@yic.ac.cn.
authors.forename: Tanji
authors.initials: T
authors.lastname: Yin
authors.affiliation: Key Laboratory of Coastal Environmental Processes and Ecological Remediation, Yantai Institute of Coastal Zone Research (YIC), Chinese Academy of Sciences (CAS); Shandong Provincial Key Laboratory of Coastal Environmental Processes, YICCAS, Yantai 264003, Shandong, China. wqin@yic.ac.cn.
authors.forename: Wei
authors.initials: W
authors.lastname: Qin
mesh_terms: D052157:Aptamers, Nucleotide / Q000737:chemistry; D015374:Biosensing Techniques / Q000379:methods*; D004247:DNA / Q000737:chemistry*; D049329:Nanostructures / Q000737:chemistry; D011199:Potentiometry / Q000379:methods*; D044149:Vibrio alginolyticus / Q000502:physiology*
chemical_list: D052157:Aptamers, Nucleotide; D004247:DNA
keywords: DNA nanostructures; Vibrio alginolyticus; magnetic beads; potentiometric aptasensing; protamine