title: Robust Synthetic Circuits for Two-Dimensional Control of Gene Expression in Yeast.
abstract: Cellular phenotypes are the result of complex interactions between many components. Understanding and predicting the system level properties of the resulting networks requires the development of perturbation tools that can simultaneously and independently modulate multiple cellular variables. Here, we develop synthetic modules that use different arrangements of two transcriptional regulators to achieve either concurrent and independent control of the expression of two genes, or decoupled control of the mean and variance of a single gene. These modules constitute powerful tools to probe the quantitative attributes of network wiring and function.
authors.affiliation: Department of Biochemistry and Biophysics, University of California San Francisco , San Francisco, California 94158, United States.|California Institute for Quantitative Biosciences, University of California San Francisco , San Francisco, California 94158, United States.
authors.forename: Andrés
authors.identifier: 0000-0002-0566-4901
authors.initials: A
authors.lastname: Aranda-Díaz
authors.affiliation: Department of Biochemistry and Biophysics, University of California San Francisco , San Francisco, California 94158, United States.|California Institute for Quantitative Biosciences, University of California San Francisco , San Francisco, California 94158, United States.
authors.forename: Kieran
authors.identifier: 0000-0002-8131-3118
authors.initials: K
authors.lastname: Mace
authors.affiliation: Department of Biochemistry and Biophysics, University of California San Francisco , San Francisco, California 94158, United States.|California Institute for Quantitative Biosciences, University of California San Francisco , San Francisco, California 94158, United States.
authors.forename: Ignacio
authors.initials: I
authors.lastname: Zuleta
authors.affiliation: Department of Biochemistry and Biophysics, University of California San Francisco , San Francisco, California 94158, United States.|California Institute for Quantitative Biosciences, University of California San Francisco , San Francisco, California 94158, United States.
authors.forename: Patrick
authors.initials: P
authors.lastname: Harrigan
authors.affiliation: Department of Biochemistry and Biophysics, University of California San Francisco , San Francisco, California 94158, United States.|California Institute for Quantitative Biosciences, University of California San Francisco , San Francisco, California 94158, United States.
authors.forename: Hana
authors.initials: H
authors.lastname: El-Samad
mesh_terms: D003198:Computer Simulation; D015870:Gene Expression / Q000235:genetics*; D005786:Gene Expression Regulation / Q000235:genetics*; D053263:Gene Regulatory Networks / Q000235:genetics*; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D014158:Transcription, Genetic / Q000235:genetics
keywords: control; mating pathway; noise; orthogonal; sic1