title: Evaluation of DNA-damaging potential of bisphenol A and its selected analogs in human peripheral blood mononuclear cells (in vitro study).
abstract: In the present study, we have investigated DNA-damaging potential of BPA and its analogs, i.e. bisphenol S (BPS), bisphenol F (BPF) and bisphenol AF (BPAF) in human peripheral blood mononuclear cells (PBMCs) using the alkaline and neutral versions of the comet assay, which allowed to evaluate DNA single strand-breaks (SSBs) and double strand-breaks (DSBs). The use of the alkaline version of comet assay made also possible to analyze the kinetics of DNA repair in PBMCs after exposure of the cells to BPA or its analogs. We have observed an increase in DNA damage in PBMCs treated with BPA or its analogs in the concentrations ranging from 0.01 to 10 μg/ml after 1 and 4 h incubation. It was noted that bisphenols studied caused DNA damage mainly via SSBs, while DNA fragmentation via double DSBs was low. The strongest changes in DNA damage were provoked by BPA and particularly BPAF, which were capable of inducing SSBs even at 0.01 μg/ml, while BPS caused the lowest changes (only at 10 μg/ml). We have also observed that PBMCs significantly repaired bisphenols-induced DNA damage but they were unable (excluding cells treated with BPS) to repair totally DNA breaks.
authors.affiliation: Department of Environmental Pollution Biophysics, Faculty of Biology and Environmental Protection, University of Łódź, Pomorska 141/143 St, 90-001 Łódź, Poland.
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Mokra
authors.affiliation: Department of Environmental Pollution Biophysics, Faculty of Biology and Environmental Protection, University of Łódź, Pomorska 141/143 St, 90-001 Łódź, Poland.
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Kuźmińska-Surowaniec
authors.affiliation: Department of Molecular Genetics, Faculty of Biology and Environmental Protection, University of Łódź, Pomorska 141/143 St, 90-001 Łódź, Poland.
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Woźniak
authors.affiliation: Department of Environmental Pollution Biophysics, Faculty of Biology and Environmental Protection, University of Łódź, Pomorska 141/143 St, 90-001 Łódź, Poland. Electronic address: michalow@biol.uni.lodz.pl.
authors.forename: Jaromir
authors.initials: J
authors.lastname: Michałowicz
mesh_terms: D000293:Adolescent; D000328:Adult; D001559:Benzhydryl Compounds / Q000097:blood / Q000737:chemistry / Q000494:pharmacology*; D002470:Cell Survival / Q000187:drug effects*; D020552:Comet Assay; D004249:DNA Damage / Q000187:drug effects*; D004260:DNA Repair / Q000187:drug effects*; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D007963:Leukocytes, Mononuclear / Q000187:drug effects*; D008297:Male; D008875:Middle Aged; D010636:Phenols / Q000097:blood / Q000737:chemistry / Q000494:pharmacology*; D055815:Young Adult; D000098826:Bisphenol A Compounds
chemical_list: D001559:Benzhydryl Compounds; D010636:Phenols; C006780:bisphenol A; D000098826:Bisphenol A Compounds
keywords: Bisphenol A; Bisphenol A analogs; Comet assay; DNA damage; DNA repair; PBMCs