title: Mature IgM-expressing plasma cells sense antigen and develop competence for cytokine production upon antigenic challenge.
abstract: Dogma holds that plasma cells, as opposed to B cells, cannot bind antigen because they have switched from expression of membrane-bound immunoglobulins (Ig) that constitute the B-cell receptor (BCR) to production of the secreted form of immunoglobulins. Here we compare the phenotypical and functional attributes of plasma cells generated by the T-cell-dependent and T-cell-independent forms of the hapten NP. We show that the nature of the secreted Ig isotype, rather than the chemical structure of the immunizing antigen, defines two functionally distinct populations of plasma cells. Fully mature IgM-expressing plasma cells resident in the bone marrow retain expression of a functional BCR, whereas their IgG+counterparts do not. Antigen boost modifies the gene expression profile of IgM+plasma cells and initiates a cytokine production program, characterized by upregulation of CCL5 and IL-10. Our results demonstrate that IgM-expressing plasma cells can sense antigen and acquire competence for cytokine production upon antigenic challenge.
authors.affiliation: CIRI, INSERM, U1111, Université Claude Bernard Lyon 1, CNRS, UMR5308, École Normale Supérieure de Lyon, Univ. Lyon, 21 Avenue Tony Garnier, 69007 Lyon, France.
authors.forename: Pascal
authors.initials: P
authors.lastname: Blanc
authors.affiliation: CIRI, INSERM, U1111, Université Claude Bernard Lyon 1, CNRS, UMR5308, École Normale Supérieure de Lyon, Univ. Lyon, 21 Avenue Tony Garnier, 69007 Lyon, France.
authors.forename: Ludovic
authors.initials: L
authors.lastname: Moro-Sibilot
authors.affiliation: CIRI, INSERM, U1111, Université Claude Bernard Lyon 1, CNRS, UMR5308, École Normale Supérieure de Lyon, Univ. Lyon, 21 Avenue Tony Garnier, 69007 Lyon, France.
authors.forename: Lucas
authors.initials: L
authors.lastname: Barthly
authors.affiliation: CIRI, INSERM, U1111, Université Claude Bernard Lyon 1, CNRS, UMR5308, École Normale Supérieure de Lyon, Univ. Lyon, 21 Avenue Tony Garnier, 69007 Lyon, France.
authors.forename: Ferdinand
authors.initials: F
authors.lastname: Jagot
authors.affiliation: CIRI, INSERM, U1111, Université Claude Bernard Lyon 1, CNRS, UMR5308, École Normale Supérieure de Lyon, Univ. Lyon, 21 Avenue Tony Garnier, 69007 Lyon, France.
authors.forename: Sébastien
authors.initials: S
authors.lastname: This
authors.affiliation: AltraBio SAS, 30 rue Pre Gaudry, 69007 Lyon, France.
authors.forename: Simon
authors.initials: S
authors.lastname: de Bernard
authors.affiliation: AltraBio SAS, 30 rue Pre Gaudry, 69007 Lyon, France.
authors.forename: Laurent
authors.initials: L
authors.lastname: Buffat
authors.affiliation: INSERM SFR Biosciences Gerland, UMS3444/US8, 50 Avenue Tony Garnier, 69007 Lyon, France.
authors.forename: Sébastien
authors.initials: S
authors.lastname: Dussurgey
authors.affiliation: eBioscience, An Affymetrix Company, 140 bis Rue de Rennes, 75006 Paris, France.
authors.forename: Renaud
authors.initials: R
authors.lastname: Colisson
authors.affiliation: Institute of Immunology, University Hospital, Albert Einstein Allee 11, Ulm 89073, Germany.
authors.forename: Elias
authors.initials: E
authors.lastname: Hobeika
authors.affiliation: INSERM, UMR917, F-35043 Rennes, France.|Pôle de Biologie, Centre Hospitalier Universitaire, 35033 Rennes, France.|Université de Rennes 1, F-35065 Rennes, France.
authors.forename: Thierry
authors.initials: T
authors.lastname: Fest
authors.affiliation: CIRI, INSERM, U1111, Université Claude Bernard Lyon 1, CNRS, UMR5308, École Normale Supérieure de Lyon, Univ. Lyon, 21 Avenue Tony Garnier, 69007 Lyon, France.
authors.forename: Morgan
authors.initials: M
authors.lastname: Taillardet
authors.affiliation: CIRI, INSERM, U1111, Université Claude Bernard Lyon 1, CNRS, UMR5308, École Normale Supérieure de Lyon, Univ. Lyon, 21 Avenue Tony Garnier, 69007 Lyon, France.
authors.forename: Olivier
authors.initials: O
authors.lastname: Thaunat
authors.affiliation: CIRI, INSERM, U1111, Université Claude Bernard Lyon 1, CNRS, UMR5308, École Normale Supérieure de Lyon, Univ. Lyon, 21 Avenue Tony Garnier, 69007 Lyon, France.
authors.forename: Antoine
authors.initials: A
authors.lastname: Sicard
authors.affiliation: CIRI, INSERM, U1111, Université Claude Bernard Lyon 1, CNRS, UMR5308, École Normale Supérieure de Lyon, Univ. Lyon, 21 Avenue Tony Garnier, 69007 Lyon, France.
authors.forename: Paul
authors.initials: P
authors.lastname: Mondière
authors.affiliation: CIRI, INSERM, U1111, Université Claude Bernard Lyon 1, CNRS, UMR5308, École Normale Supérieure de Lyon, Univ. Lyon, 21 Avenue Tony Garnier, 69007 Lyon, France.
authors.forename: Laurent
authors.initials: L
authors.lastname: Genestier
authors.affiliation: The Walter and Eliza Hall Institute of Medical Research, 1G Royal Parade, Parkville, Victoria 3052, Australia.|Department of Medical Biology, University of Melbourne, Parkville, Victoria 3010, Australia.
authors.forename: Stephen L
authors.initials: SL
authors.lastname: Nutt
authors.affiliation: CIRI, INSERM, U1111, Université Claude Bernard Lyon 1, CNRS, UMR5308, École Normale Supérieure de Lyon, Univ. Lyon, 21 Avenue Tony Garnier, 69007 Lyon, France.
authors.forename: Thierry
authors.initials: T
authors.lastname: Defrance
mesh_terms: D000818:Animals; D000921:Antibody-Producing Cells / Q000378:metabolism; D000941:Antigens / Q000378:metabolism*; D001854:Bone Marrow Cells / Q000166:cytology; D016207:Cytokines / Q000096:biosynthesis*; D003911:Dextrans / Q000378:metabolism; D020869:Gene Expression Profiling; D063990:Gene Ontology; D007075:Immunoglobulin M / Q000378:metabolism*; D008810:Mice, Inbred C57BL; D010950:Plasma Cells / Q000378:metabolism*; D011947:Receptors, Antigen, B-Cell / Q000378:metabolism
chemical_list: D000941:Antigens; D016207:Cytokines; D003911:Dextrans; D007075:Immunoglobulin M; D011947:Receptors, Antigen, B-Cell