title: Metabolic engineering of Saccharomyces cerevisiae for improvement in stresses tolerance.
abstract: Lignocellulosic biomass is an attractive low-cost feedstock for bioethanol production. During bioethanol production, Saccharomyces cerevisiae, the common used starter, faces several environmental stresses such as aldehydes, glucose, ethanol, high temperature, acid, alkaline and osmotic pressure. The aim of this study was to construct a genetic recombinant S. cerevisiae starter with high tolerance against various environmental stresses. Trehalose-6-phosphate synthase gene (tps1) and aldehyde reductase gene (ari1) were co-overexpressed in nth1 (coded for neutral trehalase gene, trehalose degrading enzyme) deleted S. cerevisiae. The engineered strain exhibited ethanol tolerance up to 14% of ethanol, while the growth of wild strain was inhibited by 6% of ethanol. Compared with the wild strain, the engineered strain showed greater ethanol yield under high stress condition induced by combining 30% glucose, 30 mM furfural and 30 mM 5-hydroxymethylfurfural (HMF).
authors.affiliation: a Department of Food and Nutrition , Providence University , Taichung , Republic of China (Taiwan).
authors.forename: Nileema R
authors.initials: NR
authors.lastname: Divate
authors.affiliation: b Department of Cosmetic Science , Providence University , Taichung , Republic of China (Taiwan).
authors.forename: Gen-Hung
authors.initials: GH
authors.lastname: Chen
authors.affiliation: a Department of Food and Nutrition , Providence University , Taichung , Republic of China (Taiwan).
authors.forename: Rupesh D
authors.initials: RD
authors.lastname: Divate
authors.affiliation: c Department of Animal Science and Biotechnology , Tunghai University , Taichung , Republic of China (Taiwan).
authors.forename: Bor-Rung
authors.initials: BR
authors.lastname: Ou
authors.affiliation: a Department of Food and Nutrition , Providence University , Taichung , Republic of China (Taiwan).
authors.forename: Yun-Chin
authors.initials: YC
authors.lastname: Chung
mesh_terms: D000431:Ethanol / Q000302:isolation & purification / Q000378:metabolism*; D024861:Genetic Enhancement / Q000379:methods*; D005947:Glucose / Q000378:metabolism*; D060847:Metabolic Engineering / Q000379:methods*; D009097:Multienzyme Complexes / Q000235:genetics*; D012441:Saccharomyces cerevisiae / Q000502:physiology*; D013312:Stress, Physiological / Q000235:genetics*; D015854:Up-Regulation / Q000235:genetics
chemical_list: D009097:Multienzyme Complexes; D000431:Ethanol; D005947:Glucose
keywords: Aldehyde reductase; Saccharomyces cerevisiae; Trehalose; Trehalose-6-phosphate synthase; ethanol production; neutral trehalase