title: Study of the Alpha-l-Fucosidase Langmuir Monolayer at the Air-Water Interface.
abstract: Alpha-l-fucosidase is a known biomarker for hepatocellular carcinoma that has shown great potential in diagnostics. Most of the focus for this enzyme has been on the free form found in serum; however, little is known of the properties of the minor portion of membrane-bound alpha-l-fucosidase. To better understand the properties of membrane-bound alpha-l-fucosidase, this enzyme was surveyed at the air-water interface. Alpha-l-fucosidase is able to form a stable Langmuir monolayer, which was confirmed through surface-pressure and surface-potential area isotherms, as well as infrared reflection-absorption spectroscopy (IRRAS). Furthermore, an interaction between the alpha-l-fucosidase Langmuir monolayer and a specific antibody for this enzyme, FUCA2, was observed.
authors.affiliation: Department of Chemistry, University of Miami , 1301 Memorial Drive, Cox Science Center, Coral Gables, Florida 33146, United States.
authors.forename: Eric
authors.initials: E
authors.lastname: Waidely
authors.affiliation: Department of Chemistry, King Abdulaziz University , P.O. Box 80200, Jeddah 21589, Kingdom of Saudi Arabia.
authors.forename: Abdulrahman O
authors.initials: AO
authors.lastname: Al-Youbi
authors.affiliation: Department of Chemistry, King Abdulaziz University , P.O. Box 80200, Jeddah 21589, Kingdom of Saudi Arabia.
authors.forename: Abdulaziz S
authors.initials: AS
authors.lastname: Bashammakh
authors.affiliation: Department of Chemistry, King Abdulaziz University , P.O. Box 80200, Jeddah 21589, Kingdom of Saudi Arabia.
authors.forename: Mohammad S
authors.initials: MS
authors.lastname: El-Shahawi
authors.affiliation: Department of Chemistry, University of Miami , 1301 Memorial Drive, Cox Science Center, Coral Gables, Florida 33146, United States.
authors.forename: Roger M
authors.identifier: 0000-0001-8836-8042
authors.initials: RM
authors.lastname: Leblanc
mesh_terms: D000327:Adsorption; D000388:Air / Q000032:analysis*; D000906:Antibodies / Q000737:chemistry*; D000918:Antibody Specificity; D011312:Pressure; D011485:Protein Binding; D013499:Surface Properties; D014867:Water / Q000737:chemistry*; D005644:alpha-L-Fucosidase / Q000737:chemistry*
chemical_list: D000906:Antibodies; D014867:Water; C000593240:FUCA2 protein, human; D005644:alpha-L-Fucosidase