title: Genome-Wide Analysis of Periodontal and Peri-Implant Cells and Tissues.
abstract: Omics analyses, including the systematic cataloging of messenger RNA and microRNA sequences or DNA methylation patterns in a cell population, organ or tissue sample, are powerful means of generating comprehensive genome-level data sets on complex diseases. We have systematically assessed the transcriptome, miRNome and methylome of gingival tissues from subjects with different diagnostic entities of periodontal disease, and studied the transcriptome of primary cells ex vivo, or in vitro after infection with periodontal pathogens. Our data further our understanding of the pathobiology of periodontal diseases and indicate that the gingival -omes translate into discernible phenotypic characteristics and possibly support an alternative, "molecular" classification of periodontitis.Here, we outline the laboratory steps required for the processing of periodontal cells and tissues for -omics analyses using current microarrays or next-generation sequencing technology.
authors.affiliation: Department of Periodontology, Operative and Preventive Dentistry, Faculty of Medicine, University of Bonn, Welschnonnenstr. 17, Bonn, D-53111, Germany. moritz.kebschull@uni-bonn.de.|Division of Periodontics, Section of Oral, Diagnostic and Rehabilitation Sciences, Columbia University College of Dental Medicine, New York, NY, USA. moritz.kebschull@uni-bonn.de.
authors.forename: Moritz
authors.initials: M
authors.lastname: Kebschull
authors.affiliation: Department of Periodontology, Operative and Preventive Dentistry, Faculty of Medicine, University of Bonn, Welschnonnenstr. 17, Bonn, D-53111, Germany.
authors.forename: Claudia
authors.initials: C
authors.lastname: Hülsmann
authors.affiliation: Department of Genomics, Institute of Human Genetics, University of Bonn, Bonn, Germany.|Human Genomics Research Group, Department of Biomedicine, University of Basel, Basel, Switzerland.
authors.forename: Per
authors.initials: P
authors.lastname: Hoffmann
authors.affiliation: Division of Periodontics, Section of Oral, Diagnostic and Rehabilitation Sciences, Columbia University College of Dental Medicine, New York, NY, USA.
authors.forename: Panos N
authors.initials: PN
authors.lastname: Papapanou
mesh_terms: D002478:Cells, Cultured; D019175:DNA Methylation; D057890:Epigenomics / Q000379:methods; D020869:Gene Expression Profiling; D055106:Genome-Wide Association Study / Q000379:methods; D005881:Gingiva / Q000378:metabolism*; D059014:High-Throughput Nucleotide Sequencing; D006801:Humans; D007963:Leukocytes, Mononuclear; D010510:Periodontal Diseases / Q000235:genetics*; D059467:Transcriptome
keywords: DNA methylation; Gene expression; Gingiva; MicroRNA; Microarray; Next-generation sequencing; Periodontal disease; Transcriptome