title: Overexpression of Semaphorin-3E enhances pancreatic cancer cell growth and associates with poor patient survival.
abstract: Semaphorin-3E (Sema3E) is a member of an axon guidance gene family, and has recently been reported to contribute to tumor progression and metastasis. However, its role in pancreatic cancer is yet unknown and uncharacterized. In this study, we showed that Sema3E is overexpressed in human pancreatic cancer, and that high Sema3E levels are associated with tumor progression and poor survival. Interestingly, we also observed Sema3E expression in the nucleus, even though Sema3E is reported to be a secreted protein. Overexpression of Sema3E in pancreatic cancer cells promoted cell proliferation and migration in vitro, and increased tumor incidence and growth in vivo. Conversely, knockout of Sema3E suppressed cancer cell proliferation and migration in vitro, and reduced tumor incidence and size in vivo. Moreover, Sema3E induced cell proliferation via acting through the MAPK/ERK pathway. Collectively, these results reveal an undiscovered role of Sema3E in promoting pancreatic cancer pathogenesis, suggesting that Sema3E may be a suitable prognostic marker and therapeutic target for pancreatic cancer.
authors.affiliation: Michael E. DeBakey Department of Surgery, Division of Surgical Research, Baylor College of Medicine, Houston, TX, USA.|Interdepartmental Program in Translational Biology and Molecular Medicine, Baylor College of Medicine, Houston, TX, USA.
authors.forename: Lin-Kin
authors.initials: LK
authors.lastname: Yong
authors.affiliation: Department of Pathology, Michael E. DeBakey VA Medical Center and Baylor College of Medicine, Houston, TX, USA.
authors.forename: Syeling
authors.initials: S
authors.lastname: Lai
authors.affiliation: Michael E. DeBakey Department of Surgery, Division of Surgical Research, Baylor College of Medicine, Houston, TX, USA.
authors.forename: Zhengdong
authors.initials: Z
authors.lastname: Liang
authors.affiliation: Michael E. DeBakey Department of Surgery, Division of Surgical Research, Baylor College of Medicine, Houston, TX, USA.
authors.forename: Ethan
authors.initials: E
authors.lastname: Poteet
authors.affiliation: Duncan Cancer Center, Baylor College of Medicine, Houston, TX, USA.|Department of Medicine, Baylor College of Medicine, Houston, TX, USA.
authors.forename: Fengju
authors.initials: F
authors.lastname: Chen
authors.affiliation: Duncan Cancer Center, Baylor College of Medicine, Houston, TX, USA.|Michael E. DeBakey Department of Surgery, Division of General Surgery, Baylor College of Medicine, Houston, TX, USA.
authors.forename: George
authors.initials: G
authors.lastname: van Buren
authors.affiliation: Duncan Cancer Center, Baylor College of Medicine, Houston, TX, USA.|Michael E. DeBakey Department of Surgery, Division of General Surgery, Baylor College of Medicine, Houston, TX, USA.
authors.forename: William
authors.initials: W
authors.lastname: Fisher
authors.affiliation: Duncan Cancer Center, Baylor College of Medicine, Houston, TX, USA.|Department of Medicine, Baylor College of Medicine, Houston, TX, USA.
authors.forename: Qianxing
authors.initials: Q
authors.lastname: Mo
authors.affiliation: Michael E. DeBakey Department of Surgery, Division of Surgical Research, Baylor College of Medicine, Houston, TX, USA.|Duncan Cancer Center, Baylor College of Medicine, Houston, TX, USA.
authors.forename: Changyi
authors.initials: C
authors.lastname: Chen
authors.affiliation: Michael E. DeBakey Department of Surgery, Division of Surgical Research, Baylor College of Medicine, Houston, TX, USA.|Duncan Cancer Center, Baylor College of Medicine, Houston, TX, USA.|Center for Translational Research on Inflammatory Diseases (CTRID), Michael E. DeBakey VA Medical Center, Houston, TX, USA.
authors.forename: Qizhi
authors.initials: Q
authors.lastname: Yao
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D048049:Extracellular Signal-Regulated MAP Kinases / Q000502:physiology; D005260:Female; D006801:Humans; D020935:MAP Kinase Signaling System / Q000502:physiology; D051379:Mice; D010190:Pancreatic Neoplasms / Q000401:mortality / Q000473:pathology*; D039961:Semaphorins / Q000032:analysis / Q000502:physiology*
chemical_list: C494123:SEMA3E protein, human; D039961:Semaphorins; D048049:Extracellular Signal-Regulated MAP Kinases
keywords: pancreatic cancer; semaphorin 3E