title: Cell-Based Assays to Study ERK Pathway/Caveolin1 Interactions.
abstract: Caveolin1, the main component of caveolae, plays a major role in regulating cell motility, gene expression, and cytoskeleton remodeling downstream of many membrane receptors. Here, we summarize different techniques set up to study changes in cell morphology and cell motility regulated by ERK/caveolin1 interactions during induction of epithelial mesenchymal transition (EMT) in mesothelial cells (MCs).
authors.affiliation: Integrin Signaling Laboratory, Cell Biology & Physiology Program, Cell & Developmental Biology Area, Centro Nacional de Investigaciones Cardiovasculares, Melchor Fernandez Almagro, 3, Madrid, 28029, Spain. raffaele.strippoli@uniroma1.it.|Department of Cellular Biotechnologies and Haematology, Sapienza University of Rome, Viale Regina Elena 324, 00185, Rome, Italy. raffaele.strippoli@uniroma1.it.
authors.forename: Raffaele
authors.initials: R
authors.lastname: Strippoli
authors.affiliation: Integrin Signaling Laboratory, Cell Biology & Physiology Program, Cell & Developmental Biology Area, Centro Nacional de Investigaciones Cardiovasculares, Melchor Fernandez Almagro, 3, Madrid, 28029, Spain.
authors.forename: Asier
authors.initials: A
authors.lastname: Echarri
authors.affiliation: Integrin Signaling Laboratory, Cell Biology & Physiology Program, Cell & Developmental Biology Area, Centro Nacional de Investigaciones Cardiovasculares, Melchor Fernandez Almagro, 3, Madrid, 28029, Spain. madelpozo@cnic.es.
authors.forename: Miguel Angel
authors.initials: MA
authors.lastname: Del Pozo
mesh_terms: D000818:Animals; D051242:Caveolin 1 / Q000378:metabolism*; D018929:Cell Culture Techniques; D002465:Cell Movement; D048430:Cell Shape; D004847:Epithelial Cells / Q000166:cytology / Q000378:metabolism; D058750:Epithelial-Mesenchymal Transition; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism*; D051379:Mice; D010529:Peritoneal Cavity; D011485:Protein Binding; D015398:Signal Transduction
chemical_list: D051242:Caveolin 1; D048049:Extracellular Signal-Regulated MAP Kinases
keywords: Caveolin; Cell height; Cell morphology; Cell motility; ERK; Mesothelial cells