title: Chromosome Bandings.
abstract: Chromosome banding is an essential technique used in chromosome karyotyping to identify normal and abnormal chromosomes for clinical and research purposes. Giemsa (G)-, reverse (R)-, and centromere (C)-banding are the most commonly dye-based chromosome-banding techniques. G-banding involves the staining of trypsin-treated chromosomes and R-banding involves denaturing in hot acidic saline followed by Giemsa staining. C-banding is specifically used for identifying heterochromatin by denaturing chromosomes in a saturated alkaline solution followed by Giemsa staining. Different banding techniques may be selected for the identification of chromosomes.
authors.affiliation: Central Laboratory, Fujian Medical University Affiliated Union Hospital, 29 Xinquan Road, Fuzhou, 350001, P. R. China. huanghuif@126.com.
authors.forename: Huifang
authors.initials: H
authors.lastname: Huang
authors.affiliation: Fujian Institute of Hematology, Fujian Medical University Affiliated Union Hospital, Fuzhou, P. R. China.
authors.forename: Jiadi
authors.initials: J
authors.lastname: Chen
mesh_terms: D002871:Chromosome Banding / Q000379:methods*; D006801:Humans; D007621:Karyotyping; D008677:Metaphase / Q000235:genetics
keywords: C-banding; Chromosome banding; G-banding; Karyotyping; R-banding