title: Identification of sea bass pIgR shows its interaction with vitellogenin inducing antibody-like activities in HEK 293T cells.
abstract: Fish vitellogenin (Vg) has been shown to mediate the phagocytosis via interaction with a Fcγ-like phagocytic receptor on macrophages, but identification of such a receptor and its functional characterization remains lacking. In this study, we isolated a cDNA of polymeric immunoglobulin receptor (pIgR) from sea bass, which encoded a single-spanning transmembrane protein of 326 amino acids including a 21-amino acid signal peptide, an extracellular region, a transmembrane region and a 36-amino acid intracellular region included two Ig-like domains (ILDs), and was expressed in multiple lymphoid organs. We then showed that recombinant extracellular domain of sea bass pIgR was capable of binding to Vg as well as IgG and IgM. We also showed that Vg as well as IgG and IgM interacted with pIgR-expressing HEK 293T cells. Importantly, we demonstrated that Vg as well as IgG and IgM were all capable of enhancing phagocytosis by HEK 293T cells and inducing expression of tnf-α and il-1β, via interacting with pIgR. Collectively, these results suggest that fish Vg, analogous to IgG and IgM, can interact with pIgR and result in similar down-stream immune responses, providing an additional evidence that Vg plays an antibody-like role.
authors.affiliation: Laboratory for Evolution & Development, Institute of Evolution & Marine Biodiversity, China.
authors.forename: Shuangshuang
authors.initials: S
authors.lastname: Yang
authors.affiliation: Laboratory for Evolution & Development, Institute of Evolution & Marine Biodiversity, China.
authors.forename: Shousheng
authors.initials: S
authors.lastname: Liu
authors.affiliation: Laboratory for Evolution & Development, Institute of Evolution & Marine Biodiversity, China.
authors.forename: Baozhen
authors.initials: B
authors.lastname: Qu
authors.affiliation: Laboratory for Evolution & Development, Institute of Evolution & Marine Biodiversity, China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Dong
authors.affiliation: Laboratory for Evolution & Development, Institute of Evolution & Marine Biodiversity, China; Department of Marine Biology, Ocean University of China, China. Electronic address: sczhang@ouc.edu.cn.
authors.forename: Shicui
authors.initials: S
authors.lastname: Zhang
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001492:Bass / Q000235:genetics* / Q000276:immunology* / Q000378:metabolism; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics / Q000378:metabolism; D029941:Fish Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D005786:Gene Expression Regulation; D057809:HEK293 Cells; D006801:Humans; D007113:Immunity, Innate; D009928:Organ Specificity; D010802:Phylogeny; D000072417:Protein Domains; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction / Q000662:veterinary; D019056:Receptors, Polymeric Immunoglobulin / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D016415:Sequence Alignment / Q000662:veterinary
chemical_list: D018076:DNA, Complementary; D029941:Fish Proteins; D012333:RNA, Messenger; D019056:Receptors, Polymeric Immunoglobulin
keywords: IgG; IgM; Polymeric immunoglobulin receptor; Sea bass; Vitellogenin