title: In Vitro Selective Growth-Inhibitory Effect of 8-Hydroxyquinoline on Clostridium perfringens versus Bifidobacteria in a Medium Containing Chicken Ileal Digesta.
abstract: Clostridium perfringens-induced necrotic enteritis is generally controlled by antibiotics. However, because of increasing antibiotic resistance, other antibacterial agents are required, preferably ones that do not affect the beneficial intestinal microbiota of the host. This study evaluated the in vitro selective growth-inhibitory effect of 8-hydroxyquinoline (8HQ) on C. perfringens vs. bifidobacteria in a medium containing chicken ileal digesta. Prior to the experiments, the minimum inhibitory concentrations of 8HQ and penicillin G were determined by broth microdilution assay. The minimum inhibitory concentration values of 8HQ for C. perfringens were 16-32 times lower than the values for bifidobacteria. Treatment of autoclaved and non-autoclaved chicken ileal digesta with 8HQ showed a selective anticlostridial effect. After incubation of C. perfringens with autoclaved ileal digesta for 3 h, all 8HQ concentrations tested (32-2048 μg/mL) significantly reduced C. perfringens bacterial count. In contrast, the same treatment had no or only a slight effect on bifidobacteria counts. Unlike 8HQ, penicillin G did not exhibit any selectivity. Similar results were obtained after incubation for 24 h. In non-autoclaved ileal digesta, all 8HQ concentrations tested significantly reduced C. perfringens bacterial counts after incubation for 30 min and 3 h, while no effect was observed on bifidobacteria. These results suggest that 8HQ may serve as a prospective veterinary compound for use against necrotic enteritis in poultry.
authors.affiliation: Department of Physiology of Nutrition and Quality of Animal Products, Institute of Animal Science, Prague, Czech Republic.|Department of Microbiology, Nutrition and Dietetics, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences, Prague, Czech Republic.
authors.forename: Eva
authors.initials: E
authors.lastname: Skrivanova
authors.affiliation: Department of Pathology, Bacteriology and Poultry Diseases, Ghent University, Merelbeke, Belgium.
authors.forename: Filip
authors.initials: F
authors.lastname: Van Immerseel
authors.affiliation: Department of Physiology of Nutrition and Quality of Animal Products, Institute of Animal Science, Prague, Czech Republic.|Department of Microbiology, Nutrition and Dietetics, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences, Prague, Czech Republic.
authors.forename: Petra
authors.initials: P
authors.lastname: Hovorkova
authors.affiliation: Department of Crop Sciences and Agroforestry, Faculty of Tropical AgriSciences, Czech University of Life Sciences, Prague, Czech Republic.
authors.forename: Ladislav
authors.initials: L
authors.lastname: Kokoska
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001644:Bifidobacterium / Q000187:drug effects*; D002645:Chickens / Q000382:microbiology*; D003015:Clostridium Infections / Q000517:prevention & control / Q000662:veterinary* / Q000821:virology; D003016:Clostridium perfringens / Q000187:drug effects*; D007082:Ileum / Q000382:microbiology; D015125:Oxyquinoline / Q000494:pharmacology*; D011201:Poultry Diseases / Q000517:prevention & control* / Q000821:virology
chemical_list: D000900:Anti-Bacterial Agents; D015125:Oxyquinoline