title: Suppression of bromodomain-containing protein 4 by shRNA: A new approach for cancer treatment.
abstract: MYC is a transcription factor coding gene that is believed to control 15% of the genes in the entire human genome. The central role of c-MYC in cancer pathogenesis makes it a major therapeutic target in field of anticancer agent development.
  
  
  
  We targeted the acetyl-lysine binding modules or bromodomains, which are associated with c-MYC transcriptional activation.
  
  
  
  Sequence specific inhibition of BET bromodomains with small hairpin RNAs (shRNAs) resulted in cessation of cellular proliferation in different cancer cell lines. Unlike previous studies on inhibition of bromodomains with selective small-molecule inhibitors, our study revealed the significant role of BET bromodomains in solid tumours and also highlighted the ease of RNA interference (RNAi) methodology for inhibition of bromodomain translation.
  
  
  
  The degree of influence of BET bromodomain inhibition on proliferation in five cancer cell lines established it as the major target in malignancies characterized by activation of c-MYC.
authors.affiliation: . tkaya2013@stu.turgutozal.edu.tr.
authors.forename: Turan
authors.initials: T
authors.lastname: Kaya
authors.forename: Berke
authors.initials: B
authors.lastname: Kahraman
authors.forename: Nurgeldi
authors.initials: N
authors.lastname: Bazarov
authors.forename: Alperen S
authors.initials: AS
authors.lastname: Toker
authors.forename: Ayse
authors.initials: A
authors.lastname: Celik
authors.forename: Sadik
authors.initials: S
authors.lastname: Cigdem
authors.forename: Esra
authors.initials: E
authors.lastname: Gunduz
mesh_terms: D000970:Antineoplastic Agents / Q000737:chemistry; D018797:Cell Cycle Proteins; D003001:Cloning, Molecular; D015972:Gene Expression Regulation, Neoplastic; D015316:Genetic Therapy / Q000379:methods; D015894:Genome, Human; D057809:HEK293 Cells; D019073:HT29 Cells; D006367:HeLa Cells; D056945:Hep G2 Cells; D006801:Humans; D008239:Lysine / Q000737:chemistry; D061986:MCF-7 Cells; D009369:Neoplasms / Q000235:genetics* / Q000378:metabolism; D009687:Nuclear Proteins / Q000235:genetics* / Q000378:metabolism; D010957:Plasmids / Q000378:metabolism; D000072417:Protein Domains; D016271:Proto-Oncogene Proteins c-myc / Q000235:genetics / Q000378:metabolism*; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000032:analysis*; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism; D015533:Transcriptional Activation; D000092468:Bromodomain Containing Proteins
chemical_list: D000970:Antineoplastic Agents; D018797:Cell Cycle Proteins; D008239:Lysine; D009687:Nuclear Proteins; D016271:Proto-Oncogene Proteins c-myc; D034741:RNA, Small Interfering; D014157:Transcription Factors; C441289:BRD4 protein, human; D000092468:Bromodomain Containing Proteins; C489427:MYC protein, human