title: From rotten grapes to industrial exploitation: Komagataeibacter europaeus SGP37, a micro-factory for macroscale production of bacterial nanocellulose.
abstract: Bacterial nanocellulose (BNC), being ultrapure and unique in its properties, is a booming and ageless precursor of several breakthrough technologies of materials sciences; however, its low yield and high cost has created a challenge for its usage at industrial level. Herein, we report a novel, high yielding bacterial cell factory Komagataeibacter europaeus SGP37, isolated from rotten grapes, for the production of high quality and value added BNC. The strain was kinetically analyzed to evaluate BNC production under different physiological conditions and had demonstrated the production of 9.98±0.24gL-1BNC at the expense of 12.08±1.94gL-1sugar following 2 weeks of cultivation, thus having the conversion yield of 0.82g BNC/g sugar which seems to be the maximum reported yield so far. The analysis of produced pellicle using FTIR,13C CP MAS NMR, FE-SEM, XRD and TGA had shown similar structural, morphological and chemical characteristics with that of bacterial nanocellulose. Thus, K. europaeus SGP37 appears to be a potential strain and may offer a promising platform for industrial scale production of nanocelluloses.
authors.affiliation: Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee 247667, India. Electronic address: swatibt18@gmail.com.
authors.forename: Swati
authors.initials: S
authors.lastname: Dubey
authors.affiliation: Centre of Biomedical Research, SGPGIMS Campus, Rae Bareli Road, Lucknow 226014, India.
authors.forename: Raj Kumar
authors.initials: RK
authors.lastname: Sharma
authors.affiliation: Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee 247667, India.
authors.forename: Pragati
authors.initials: P
authors.lastname: Agarwal
authors.affiliation: Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee 247667, India.
authors.forename: Jyoti
authors.initials: J
authors.lastname: Singh
authors.affiliation: Centre of Biomedical Research, SGPGIMS Campus, Rae Bareli Road, Lucknow 226014, India.
authors.forename: Neeraj
authors.initials: N
authors.lastname: Sinha
authors.affiliation: Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee 247667, India. Electronic address: rpsbsfbs@iitr.ac.in.
authors.forename: R P
authors.initials: RP
authors.lastname: Singh
mesh_terms: D016947:Acetobacteraceae / Q000378:metabolism*; D001709:Biotechnology / Q000191:economics / Q000379:methods*; D002482:Cellulose / Q000096:biosynthesis* / Q000737:chemistry*; D055598:Chemical Phenomena; D007221:Industry / Q000191:economics; D049329:Nanostructures; D027843:Vitis / Q000737:chemistry*
chemical_list: D002482:Cellulose
keywords: Bacterial nanocellulose; Conversion yield; Komagataeibacter europaeus; Rotten grapes