title: Triolein reduces MMP-1 upregulation in dermal fibroblasts generated by ROS production in UVB-irradiated keratinocytes.
abstract: BACKGROUND
  Cytokine production and oxidative stress generated by ultraviolet radiation B (UVB) skin exposure are main factors of skin photoaging. Interleukin-6 (IL-6) produced by irradiated keratinocytes is proposed to have a role in metalloproteinases (MMPs) expression activation in dermal fibroblasts.
  
  
  OBJECTIVE
  We examined the effect of triolein treatment of UVB-irradiated keratinocytes on MMP1 (interstitial collagenase) expression response of dermal fibroblasts. We assayed UVB-irradiated keratinocytes soluble signals, mainly IL-6 and reactive oxygen species (ROS).
  
  
  METHODS
  IL-6 expression and ROS generation were assayed in UVB-irradiated keratinocytes. MMP1 mRNA expression response was assayed in fibroblasts grown in keratinocytes conditioned medium. We evaluated the effect of treating keratinocytes with triolein on IL-6 expression and ROS generation in keratinocytes, and MMP1 expression in fibroblasts.
  
  
  RESULTS
  The irradiation of epidermal cells with sublethal UVB doses increased IL-6 expression and ROS generation. Conditioned culture medium collected from keratinocytes was used to culture dermal fibroblasts. MMP1 mRNA expression increase was observed in fibroblasts cultured in medium collected from UVB-irradiated keratinocytes. Triolein treatment reduced the IL-6 expression and ROS generation in keratinocytes and this effect was reflected in downregulation of MMP1 expression in fibroblasts.
  
  
  CONCLUSIONS
  Triolein reduces both the expression of IL-6 and ROS generation in irradiated keratinocytes. It seems to exert an anti-inflammatory and anti-oxidative stress effect on irradiated keratinocytes that in turn reduces MMP1 expression in dermal fibroblasts. Collectively, these results indicate that triolein could act as a photoprotective agent.
authors.affiliation: Fundación Pablo Cassará-Instituto de Ciencia y Tecnología Dr. César Milstein, Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Saladillo 2468 C1440FFX, Ciudad de Buenos Aires, Argentina. Electronic address: gleiros@fundacioncassara.org.ar.
authors.forename: Gustavo J
authors.initials: GJ
authors.lastname: Leirós
authors.affiliation: Fundación Pablo Cassará-Instituto de Ciencia y Tecnología Dr. César Milstein, Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Saladillo 2468 C1440FFX, Ciudad de Buenos Aires, Argentina. Electronic address: gkusinsky@fundacioncassara.org.ar.
authors.forename: Ana Gabriela
authors.initials: AG
authors.lastname: Kusinsky
authors.affiliation: Fundación Pablo Cassará-Instituto de Ciencia y Tecnología Dr. César Milstein, Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Saladillo 2468 C1440FFX, Ciudad de Buenos Aires, Argentina. Electronic address: mbalana@fundacioncassara.org.ar.
authors.forename: María Eugenia
authors.initials: ME
authors.lastname: Balañá
authors.affiliation: Fundación Pablo Cassará-Instituto de Ciencia y Tecnología Dr. César Milstein, Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Saladillo 2468 C1440FFX, Ciudad de Buenos Aires, Argentina. Electronic address: khagelin@fundacioncassara.org.ar.
authors.forename: Karin
authors.initials: K
authors.lastname: Hagelin
mesh_terms: D000975:Antioxidants / Q000494:pharmacology; D002460:Cell Line; D017077:Culture Media, Conditioned; D020405:Dermis / Q000166:cytology / Q000187:drug effects / Q000201:enzymology / Q000528:radiation effects; D015536:Down-Regulation; D005347:Fibroblasts / Q000187:drug effects / Q000201:enzymology; D006801:Humans; D015850:Interleukin-6 / Q000378:metabolism; D015603:Keratinocytes / Q000187:drug effects* / Q000201:enzymology / Q000528:radiation effects; D020781:Matrix Metalloproteinase 1 / Q000378:metabolism*; D018384:Oxidative Stress / Q000187:drug effects; D012333:RNA, Messenger / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism*; D015595:Skin Aging / Q000187:drug effects / Q000528:radiation effects; D013473:Sunscreening Agents / Q000494:pharmacology*; D014304:Triolein / Q000494:pharmacology*; D014466:Ultraviolet Rays / Q000009:adverse effects; D015854:Up-Regulation
chemical_list: D000975:Antioxidants; D017077:Culture Media, Conditioned; C508600:IL6 protein, human; D015850:Interleukin-6; D012333:RNA, Messenger; D017382:Reactive Oxygen Species; D013473:Sunscreening Agents; D014304:Triolein; C522360:MMP1 protein, human; D020781:Matrix Metalloproteinase 1
keywords: MMP1; ROS; Triolein IL-6; UVB