title: Sodium alginate and gum acacia hydrogels of ZnO nanoparticles show wound healing effect on fibroblast cells.
abstract: An ideal biomaterial for wound dressing applications should possess antibacterial and anti-inflammatory properties without any toxicity to the host cells while providing the maximum healing activity. Zinc oxide nanoparticles (ZnONPs) possess antimicrobial activity and enhance wound healing, but the questions regarding their safety arise before application to the biological systems. We synthesized ZnONPs-loaded-sodium alginate-gum acacia hydrogels (SAGA-ZnONPs) by cross linking hydroxyl groups of the polymers sodium alginate and gum acacia with the aldehyde group of gluteradehyde. Here, we report the wound healing properties of sodium alginate/gum acacia/ZnONPs, circumventing the toxicity of ZnONPs simultaneously. We demonstrated the concentration-dependent zones of inhibition in treated cultures of Pseudomonas aerigunosa and Bacillus cereus and biocompatability on peripheral blood mononuclear/fibroblast cells. SAGA-ZnONPs hydrogels showed a healing effect at a low concentration of ZnONPs using sheep fibroblast cells. Our findings suggest that high concentrations of ZnONPs were toxic to cells but SAGA-ZnONPs hydrogels significantly reduced the toxicity and preserved the beneficial antibacterial and healing effect.
authors.affiliation: ICAR-National Research Centre on Equines, Sirsa Road, Hisar 125001, Haryana, India; ICAR-Indian Veterinary Research Institute, Bareilly, Uttar Pradesh, India.
authors.forename: R
authors.initials: R
authors.lastname: Raguvaran
authors.affiliation: ICAR-National Research Centre on Equines, Sirsa Road, Hisar 125001, Haryana, India.
authors.forename: Balvinder K
authors.initials: BK
authors.lastname: Manuja
authors.affiliation: ICAR-National Research Centre on Equines, Sirsa Road, Hisar 125001, Haryana, India.
authors.forename: Meenu
authors.initials: M
authors.lastname: Chopra
authors.affiliation: Department of Bio & Nano Technology, Guru Jambheshwar University of Science and Technology, Hisar 125001, Haryana, India.
authors.forename: Rajesh
authors.initials: R
authors.lastname: Thakur
authors.affiliation: ICAR-National Research Centre on Equines, Sirsa Road, Hisar 125001, Haryana, India.
authors.forename: Taruna
authors.initials: T
authors.lastname: Anand
authors.affiliation: Electron Microscopy and Nanoscience Laboratory, Punjab Agricultural University, Ludhiana, Punjab, India.
authors.forename: Anu
authors.initials: A
authors.lastname: Kalia
authors.affiliation: ICAR-National Research Centre on Equines, Sirsa Road, Hisar 125001, Haryana, India. Electronic address: amanuja@rediffmail.com.
authors.forename: Anju
authors.initials: A
authors.lastname: Manuja
mesh_terms: D000464:Alginates / Q000737:chemistry*; D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology; D049109:Cell Proliferation / Q000187:drug effects; D005347:Fibroblasts / Q000166:cytology* / Q000187:drug effects*; D020723:Glucuronic Acid / Q000737:chemistry; D006170:Gum Arabic / Q000737:chemistry*; D006603:Hexuronic Acids / Q000737:chemistry; D020100:Hydrogels / Q000737:chemistry / Q000494:pharmacology*; D007963:Leukocytes, Mononuclear / Q000166:cytology / Q000187:drug effects; D053758:Nanoparticles / Q000737:chemistry*; D011550:Pseudomonas aeruginosa / Q000235:genetics; D012756:Sheep; D015034:Zinc Oxide / Q000737:chemistry*
chemical_list: D000464:Alginates; D000900:Anti-Bacterial Agents; D006603:Hexuronic Acids; D020100:Hydrogels; D020723:Glucuronic Acid; D006170:Gum Arabic; D015034:Zinc Oxide
keywords: Fibroblast cells; Gum acacia; Hydrogels; Nanoparticles; Sodium alginate; Zinc oxide