title: Transcriptomic characterization and curation of candidate neuropeptides regulating reproduction in the eyestalk ganglia of the Australian crayfish, Cherax quadricarinatus.
abstract: The Australian redclaw crayfish (Cherax quadricarinatus) has recently received attention as an emerging candidate for sustainable aquaculture production in Australia and worldwide. More importantly, C. quadricarinatus serves as a good model organism for the commercially important group of decapod crustaceans as it is distributed worldwide, easy to maintain in the laboratory and its reproductive cycle has been well documented. In order to better understand the key reproduction and development regulating mechanisms in decapod crustaceans, the molecular toolkit available for model organisms such as C. quadricarinatus must be expanded. However, there has been no study undertaken to establish the C. quadricarinatus neuropeptidome. Here we report a comprehensive study of the neuropeptide genes expressed in the eyestalk in the Australian crayfish C. quadricarinatus. We characterised 53 putative neuropeptide-encoding transcripts based on key features of neuropeptides as characterised in other species. Of those, 14 neuropeptides implicated in reproduction regulation were chosen for assessment of their tissue distribution using RT-PCR. Further insights are discussed in relation to current knowledge of neuropeptides in other species and potential follow up studies. Overall, the resulting data lays the foundation for future gene-based neuroendocrinology studies in C. quadricarinatus.
authors.affiliation: GeneCology Research Centre, Faculty of Science, Health, Education and Engineering, University of the Sunshine Coast, Sunshine Coast, Queensland, Australia.
authors.forename: Tuan Viet
authors.initials: TV
authors.lastname: Nguyen
authors.affiliation: GeneCology Research Centre, Faculty of Science, Health, Education and Engineering, University of the Sunshine Coast, Sunshine Coast, Queensland, Australia.
authors.forename: Scott F
authors.initials: SF
authors.lastname: Cummins
authors.affiliation: GeneCology Research Centre, Faculty of Science, Health, Education and Engineering, University of the Sunshine Coast, Sunshine Coast, Queensland, Australia.
authors.forename: Abigail
authors.initials: A
authors.lastname: Elizur
authors.affiliation: GeneCology Research Centre, Faculty of Science, Health, Education and Engineering, University of the Sunshine Coast, Sunshine Coast, Queensland, Australia.
authors.forename: Tomer
authors.initials: T
authors.lastname: Ventura
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D003400:Astacoidea / Q000145:classification / Q000235:genetics*; D001315:Australia; D019295:Computational Biology / Q000379:methods; D005724:Ganglia / Q000378:metabolism*; D020869:Gene Expression Profiling; D059014:High-Throughput Nucleotide Sequencing; D009479:Neuropeptides / Q000235:genetics*; D009928:Organ Specificity / Q000235:genetics; D010802:Phylogeny; D059467:Transcriptome
chemical_list: D009479:Neuropeptides