title: Pea3 Transcription Factors, Etv4 and Etv5, Are Required for Proper Hippocampal Dendrite Development and Plasticity.
abstract: The proper formation and morphogenesis of dendrites is essential to the establishment of neuronal connectivity. We report that 2 members of the Pea3 family of transcription factors, Etv4 and Etv5, are expressed in hippocampal neurons during the main period of dendritogenesis, suggesting that they have a function in dendrite development. Here, we show that these transcription factors are physiological regulators of growth and arborization of pyramidal cell dendrites in the developing hippocampus. Gain and loss of function assays indicate that Etv4 and Etv5 are required for proper development of hippocampal dendritic arbors and spines. We have found that in vivo deletion of either Etv4 or Etv5 in hippocampal neurons causes deficits in dendrite size and complexity, which are associated with impaired cognitive function. Additionally, our data support the idea that Etv4 and Etv5 are part of a brain-derived neurotrophic factor-mediated transcriptional program required for proper hippocampal dendrite connectivity and plasticity.
authors.affiliation: Division of Molecular and Cellular Neuroscience, Institute of Cell Biology and Neuroscience (IBCN)-CONICET, School of Medicine. University of Buenos Aires (UBA), Buenos Aires, Argentina.
authors.forename: Paula Aldana
authors.initials: PA
authors.lastname: Fontanet
authors.affiliation: Division of Molecular and Cellular Neuroscience, Institute of Cell Biology and Neuroscience (IBCN)-CONICET, School of Medicine. University of Buenos Aires (UBA), Buenos Aires, Argentina.
authors.forename: Antonella Soledad
authors.initials: AS
authors.lastname: Ríos
authors.affiliation: Division of Molecular and Cellular Neuroscience, Institute of Cell Biology and Neuroscience (IBCN)-CONICET, School of Medicine. University of Buenos Aires (UBA), Buenos Aires, Argentina.
authors.forename: Fernando Cruz
authors.initials: FC
authors.lastname: Alsina
authors.affiliation: Division of Molecular and Cellular Neuroscience, Institute of Cell Biology and Neuroscience (IBCN)-CONICET, School of Medicine. University of Buenos Aires (UBA), Buenos Aires, Argentina.
authors.forename: Gustavo
authors.initials: G
authors.lastname: Paratcha
authors.affiliation: Division of Molecular and Cellular Neuroscience, Institute of Cell Biology and Neuroscience (IBCN)-CONICET, School of Medicine. University of Buenos Aires (UBA), Buenos Aires, Argentina.
authors.forename: Fernanda
authors.initials: F
authors.lastname: Ledda
mesh_terms: D000818:Animals; D019208:Brain-Derived Neurotrophic Factor / Q000378:metabolism; D002478:Cells, Cultured; D003071:Cognition / Q000502:physiology; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D003712:Dendrites / Q000378:metabolism*; D006624:Hippocampus / Q000254:growth & development / Q000378:metabolism*; D008822:Mice, Transgenic; D000071446:Neuronal Outgrowth / Q000502:physiology; D009473:Neuronal Plasticity / Q000502:physiology; D016716:PC12 Cells; D050783:Proto-Oncogene Proteins c-ets / Q000235:genetics / Q000378:metabolism*; D051381:Rats; D015534:Trans-Activators / Q000378:metabolism*; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*
chemical_list: D019208:Brain-Derived Neurotrophic Factor; D004268:DNA-Binding Proteins; C510951:ETV4 protein, rat; C540571:Etv4 protein, mouse; C513242:Etv5 protein, mouse; C513243:Etv5 protein, rat; D050783:Proto-Oncogene Proteins c-ets; D015534:Trans-Activators; D014157:Transcription Factors
keywords: BDNF; dendrite architecture; dendritic spine development; functional plasticity; hippocampal development