title: A strategy to modulate the electrophoretic behavior in plastic microchips using sodium polystyrene sulfonate.
abstract: Plastic microchips have been broadly used as disposable microfluidic devices, but the poorly defined surface properties limit their application. Herein, we proved that an anionic polymer could be used as the background electrolyte (BGE) to provide a strong and stable cathodic electroosmotic flow (EOF) and modulate the electrophoretic behavior for efficient separation in relative thicker microchannels (∼75μm id). A cathodic EOF of ∼3.3×10-4cm2V-1s-1was maintained using sodium polystyrene sulfonate (PSSNa) with a molecular weight of 5×105as the BGE, which ensured fluorescein isothiocyanate labeled biogenic amines (BAs) appeared ahead of other components in the electropherograms obtained with microchips of cyclic olefin copolymer. Four selected BAs appeared within 50s and theoretical plate numbers of 8.0×105/m were achieved. The role of PSSNa was evaluated with streaming potential, dynamic light scattering, contact angle and atomic force microscopy. Its functionalities as surface modifier, viscosity regulator and pseudostationary phase were also confirmed. The proposed electrophoretic method was applied in the fast determination of BAs in fish meat samples.
authors.affiliation: State Key Laboratory of Applied Organic Chemistry, Key Laboratory of Nonferrous Metals Chemistry and Resources Utilization of Gansu Province, Department of Chemistry, Lanzhou University, Lanzhou, Gansu, 730000, China.
authors.forename: Jinxiu
authors.initials: J
authors.lastname: Guo
authors.affiliation: State Key Laboratory of Applied Organic Chemistry, Key Laboratory of Nonferrous Metals Chemistry and Resources Utilization of Gansu Province, Department of Chemistry, Lanzhou University, Lanzhou, Gansu, 730000, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Applied Organic Chemistry, Key Laboratory of Nonferrous Metals Chemistry and Resources Utilization of Gansu Province, Department of Chemistry, Lanzhou University, Lanzhou, Gansu, 730000, China.
authors.forename: Lizhi
authors.initials: L
authors.lastname: Zhao
authors.affiliation: State Key Laboratory of Applied Organic Chemistry, Key Laboratory of Nonferrous Metals Chemistry and Resources Utilization of Gansu Province, Department of Chemistry, Lanzhou University, Lanzhou, Gansu, 730000, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Sun
authors.affiliation: State Key Laboratory of Applied Organic Chemistry, Key Laboratory of Nonferrous Metals Chemistry and Resources Utilization of Gansu Province, Department of Chemistry, Lanzhou University, Lanzhou, Gansu, 730000, China.
authors.forename: Hongli
authors.initials: H
authors.lastname: Li
authors.affiliation: State Key Laboratory of Applied Organic Chemistry, Key Laboratory of Nonferrous Metals Chemistry and Resources Utilization of Gansu Province, Department of Chemistry, Lanzhou University, Lanzhou, Gansu, 730000, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Zhou
authors.affiliation: Department of Chemistry and Chemical Engineering, Beijing University of Technology, Beijing, 100124, China.
authors.forename: Xiayan
authors.initials: X
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Applied Organic Chemistry, Key Laboratory of Nonferrous Metals Chemistry and Resources Utilization of Gansu Province, Department of Chemistry, Lanzhou University, Lanzhou, Gansu, 730000, China. Electronic address: qiaoshengpu@gmail.com.
authors.forename: Qiaosheng
authors.initials: Q
authors.lastname: Pu
mesh_terms: D001679:Biogenic Amines / Q000032:analysis; D002138:Calibration; D004573:Electrolytes / Q000737:chemistry; D046209:Electrophoresis, Microchip / Q000379:methods*; D005396:Fish Products / Q000032:analysis; D016650:Fluorescein-5-isothiocyanate / Q000737:chemistry; D057446:Hydrodynamics; D057230:Limit of Detection; D008460:Meat / Q000032:analysis; D010969:Plastics / Q000737:chemistry*; D011137:Polystyrenes / Q000737:chemistry*; D012015:Reference Standards; D012044:Regression Analysis; D015203:Reproducibility of Results; D012972:Sodium Hydroxide / Q000737:chemistry
chemical_list: D001679:Biogenic Amines; D004573:Electrolytes; D010969:Plastics; D011137:Polystyrenes; D012972:Sodium Hydroxide; C003321:polystyrene sulfonic acid; D016650:Fluorescein-5-isothiocyanate
keywords: Biogenic amines; Cyclic olefin copolymer; Electroosmotic flow; Microchip electrophoresis; Sodium polystyrene sulfonate