title: Two-Step Membrane Binding of NDPK-B Induces Membrane Fluidity Decrease and Changes in Lipid Lateral Organization and Protein Cluster Formation.
abstract: Nucleoside diphosphate kinases (NDPKs) are crucial elements in a wide array of cellular physiological or pathophysiological processes such as apoptosis, proliferation, or metastasis formation. Among the NDPK isoenzymes, NDPK-B, a cytoplasmic protein, was reported to be associated with several biological membranes such as plasma or endoplasmic reticulum membranes. Using several membrane models (liposomes, lipid monolayers, and supported lipid bilayers) associated with biophysical approaches, we show that lipid membrane binding occurs in a two-step process: first, initiation by a strong electrostatic adsorption process and followed by shallow penetration of the protein within the membrane. The NDPK-B binding leads to a decrease in membrane fluidity and formation of protein patches. The ability of NDPK-B to form microdomains at the membrane level may be related to protein-protein interactions triggered by its association with anionic phospholipids. Such accumulation of NDPK-B would amplify its effects in functional platform formation and protein recruitment at the membrane.
authors.affiliation: Organisation et Dynamique des Membrane Biologiques, Institut de Chimie et Biochimie Moléculaires et Supramoléculaires, CNRS UMR 5246 ICBMS , Bâtiment Chevreul, 43 Boulevard du 11 Novembre 1918, Villeurbanne Cedex 69622, France.
authors.forename: Liberty
authors.initials: L
authors.lastname: Francois-Moutal
authors.affiliation: Nanoscience Centre, University of Cambridge , 11 J.J. Thomson Avenue Cambridge, Cambridge CB3 0FF, U.K.
authors.forename: Myriam M
authors.initials: MM
authors.lastname: Ouberai
authors.affiliation: Organisation et Dynamique des Membrane Biologiques, Institut de Chimie et Biochimie Moléculaires et Supramoléculaires, CNRS UMR 5246 ICBMS , Bâtiment Chevreul, 43 Boulevard du 11 Novembre 1918, Villeurbanne Cedex 69622, France.
authors.forename: Ofelia
authors.initials: O
authors.lastname: Maniti
authors.affiliation: Nanoscience Centre, University of Cambridge , 11 J.J. Thomson Avenue Cambridge, Cambridge CB3 0FF, U.K.
authors.forename: Mark E
authors.initials: ME
authors.lastname: Welland
authors.affiliation: Department of Biochemistry, Nencki Institute of Experimental Biology, Polish Academy of Sciences , 3 Pasteur Street, Warsaw 02-093, Poland.
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Strzelecka-Kiliszek
authors.affiliation: Department of Biochemistry, Nencki Institute of Experimental Biology, Polish Academy of Sciences , 3 Pasteur Street, Warsaw 02-093, Poland.
authors.forename: Marcin
authors.initials: M
authors.lastname: Wos
authors.affiliation: Department of Biochemistry, Nencki Institute of Experimental Biology, Polish Academy of Sciences , 3 Pasteur Street, Warsaw 02-093, Poland.
authors.forename: Slawomir
authors.initials: S
authors.lastname: Pikula
authors.affiliation: Department of Biochemistry, Nencki Institute of Experimental Biology, Polish Academy of Sciences , 3 Pasteur Street, Warsaw 02-093, Poland.
authors.forename: Joanna
authors.initials: J
authors.lastname: Bandorowicz-Pikula
authors.affiliation: Organisation et Dynamique des Membrane Biologiques, Institut de Chimie et Biochimie Moléculaires et Supramoléculaires, CNRS UMR 5246 ICBMS , Bâtiment Chevreul, 43 Boulevard du 11 Novembre 1918, Villeurbanne Cedex 69622, France.
authors.forename: Olivier
authors.initials: O
authors.lastname: Marcillat
authors.affiliation: Organisation et Dynamique des Membrane Biologiques, Institut de Chimie et Biochimie Moléculaires et Supramoléculaires, CNRS UMR 5246 ICBMS , Bâtiment Chevreul, 43 Boulevard du 11 Novembre 1918, Villeurbanne Cedex 69622, France.
authors.forename: Thierry
authors.initials: T
authors.lastname: Granjon
mesh_terms: D002462:Cell Membrane / Q000737:chemistry*; D006801:Humans; D008051:Lipid Bilayers / Q000737:chemistry*; D008560:Membrane Fluidity; D009701:Nucleoside-Diphosphate Kinase / Q000737:chemistry; D011485:Protein Binding
chemical_list: D008051:Lipid Bilayers; D009701:Nucleoside-Diphosphate Kinase; C542596:NDPK-B protein, human