title: UPLC-MS for metabolomics: a giant step forward in support of pharmaceutical research.
abstract: Metabolomics is a relatively new and rapidly growing area of post-genomic biological research. As use of metabolomics technology grows throughout the spectrum of drug discovery and development, and its applications broaden, its impact is expanding dramatically. This review seeks to provide the reader with a brief history of the development of metabolomics, its significance and strategies for conducting metabolomics studies. The most widely used analytical tools for metabolomics: NMR, LC-MS and GC-MS, are discussed along with considerations for their use. Herein, we will show how metabolomics can assist in pharmaceutical research studies, such as pharmacology and toxicology, and discuss some examples of the importance of metabolomics analysis in research and development.
authors.affiliation: School of Medicine, Department of Internal Medicine, Yale University, New Haven, CT, USA; Department of Chemistry, University of Connecticut, 55 North Eagleville Road, Storrs, CT, USA. Electronic address: ala.nassar@yale.edu.
authors.forename: Ala F
authors.initials: AF
authors.lastname: Nassar
authors.affiliation: West Campus Analytical Core, Yale University, West Haven, CT, USA.
authors.forename: Terence
authors.initials: T
authors.lastname: Wu
authors.affiliation: Yale School of Medicine, Departments of Neurology and Immunobiology, New Haven, CT 06511, USA.
authors.forename: Samuel F
authors.initials: SF
authors.lastname: Nassar
authors.affiliation: School of Medicine, Department of Internal Medicine, Yale University, New Haven, CT, USA.
authors.forename: Adam V
authors.initials: AV
authors.lastname: Wisnewski
mesh_terms: D000818:Animals; D002853:Chromatography, Liquid; D055808:Drug Discovery; D006801:Humans; D013058:Mass Spectrometry; D055432:Metabolomics; D000071220:Pharmaceutical Research