title: Spanish Broom (Spartium junceum L.) fibers impregnated with vancomycin-loaded chitosan nanoparticles as new antibacterial wound dressing: Preparation, characterization and antibacterial activity.
abstract: In this work, we propose as new wound dressing, the Spanish Broom fibers impregnated with vancomycin (VM) loaded chitosan nanoparticles. Spanish Broom fibers were extracted by patented method DiCoDe and the morphological, physical and mechanical properties were investigated. Chitosan nanoparticles were prepared by ionic gelation using different weight ratios between chitosan (CH) and tripolyphosphate (TPP). Nanoparticles were characterized in terms of size, zeta potential, yield, encapsulation efficiency, stability and drug release. Finally, the antibacterial activity against Staphylococcus aureus as well as in vitro cytotoxicity on HaCaT cells were evaluated. The best formulation CH/TPP 4:1 was selected based on the encapsulation efficiency and yield. Spanish Broom fibers impregnated with loaded nanoparticles showed an increased antibacterial activity against S. aureus compared to the same fibers containing VM without nanoparticles. Moreover, these fibers were not toxic to HaCaT keratinocytes cells. In conclusion, Spanish Broom fibers impregnated with VM loaded CH/TPP nanoparticles would appear to be a promising candidate for wound dressing application.
authors.affiliation: Department of Pharmacy and Biotechnologies, University of Bologna, Via San Donato 19/2, 40127 Bologna, Italy. Electronic address: teresa.cerchiara2@unibo.it.
authors.forename: Teresa
authors.initials: T
authors.lastname: Cerchiara
authors.affiliation: Department of Pharmacy and Biotechnologies, University of Bologna, Via San Donato 19/2, 40127 Bologna, Italy.
authors.forename: Angela
authors.initials: A
authors.lastname: Abruzzo
authors.affiliation: Department of Pharmacy and Biotechnologies, University of Bologna, Via San Donato 19/2, 40127 Bologna, Italy.
authors.forename: Rogers Alberto
authors.initials: RA
authors.lastname: Ñahui Palomino
authors.affiliation: Department of Pharmacy and Biotechnologies, University of Bologna, Via San Donato 19/2, 40127 Bologna, Italy.
authors.forename: Beatrice
authors.initials: B
authors.lastname: Vitali
authors.affiliation: Department of Chemistry and Chemical Technologies, University of Calabria, Via P. Bucci, 87036 Arcavacata di Rende (CS), Italy.
authors.forename: Renata
authors.initials: R
authors.lastname: De Rose
authors.affiliation: Department of Chemistry and Chemical Technologies, University of Calabria, Via P. Bucci, 87036 Arcavacata di Rende (CS), Italy.
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Chidichimo
authors.affiliation: Smart Materials, Istituto Italiano di Tecnologia, Via Morego, 30, 16163 Genova, Italy.
authors.forename: Luca
authors.initials: L
authors.lastname: Ceseracciu
authors.affiliation: Smart Materials, Istituto Italiano di Tecnologia, Via Morego, 30, 16163 Genova, Italy.
authors.forename: Athanassia
authors.initials: A
authors.lastname: Athanassiou
authors.affiliation: PolyCrystalLine SpA, Via F.S. Fabri 127/1, 40059, Medicina, Bologna, Italy.
authors.forename: Bruno
authors.initials: B
authors.lastname: Saladini
authors.affiliation: Department of Chemistry and Chemical Technologies, University of Calabria, Via P. Bucci, 87036 Arcavacata di Rende (CS), Italy.
authors.forename: Francesco
authors.initials: F
authors.lastname: Dalena
authors.affiliation: Department of Pharmacy and Biotechnologies, University of Bologna, Via San Donato 19/2, 40127 Bologna, Italy.
authors.forename: Federica
authors.initials: F
authors.lastname: Bigucci
authors.affiliation: Department of Pharmacy and Biotechnologies, University of Bologna, Via San Donato 19/2, 40127 Bologna, Italy.
authors.forename: Barbara
authors.initials: B
authors.lastname: Luppi
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry* / Q000494:pharmacology*; D001458:Bandages; D002626:Chemistry, Pharmaceutical / Q000379:methods; D048271:Chitosan / Q000737:chemistry*; D004337:Drug Carriers / Q000737:chemistry; D065546:Drug Liberation; D005782:Gels / Q000737:chemistry; D053758:Nanoparticles / Q000737:chemistry*; D010316:Particle Size; D028321:Plant Preparations / Q000737:chemistry / Q000494:pharmacology; D011122:Polyphosphates / Q000737:chemistry; D031302:Spartium / Q000737:chemistry*; D013211:Staphylococcus aureus / Q000187:drug effects; D014640:Vancomycin / Q000737:chemistry* / Q000494:pharmacology*
chemical_list: D000900:Anti-Bacterial Agents; D004337:Drug Carriers; D005782:Gels; D028321:Plant Preparations; D011122:Polyphosphates; D014640:Vancomycin; D048271:Chitosan; C005692:triphosphoric acid
keywords: Cellulose; Chitosan; Nanoparticles; Spanish Broom fibers; Vancomycin; Wound dressing