title: Effects of high-fat diet on plasma profiles of eicosanoid metabolites in mice.
abstract: Obesity is a serious health problem in the US and is associated with increased risks of various human diseases. To date, the mechanisms by which obesity increases the risks of a wide range of human diseases are not well understood. Here we used a LC-MS/MS-based lipidomics, which can analyze >100 bioactive lipid mediators produced by cyclooxygenase, lipoxygenase, and cytochrome P450 enzymes, to analyze plasma profiles of lipid mediators in high-fat diet induced obesity in C57BL/6 mice. Our results show that the plasma concentrations of epoxyoctadecenoic acids (EpOMEs, also termed as leukotoxins) are significantly increased in plasma of high-fat diet-fed mice, in addition, EpOMEs are among the most abundant lipid mediators detected in mouse plasma. Since substantial studies have shown that EpOMEs and their metabolites have a large array of detrimental effects on health, enhanced levels of EpOMEs could contribute to the pathology of obesity.
authors.affiliation: Department of Food Science, University of Massachusetts, Amherst, MA, 01003, USA.
authors.forename: Weicang
authors.initials: W
authors.lastname: Wang
authors.affiliation: Department of Entomology and Comprehensive Cancer Center, University of California, Davis, CA, 95616, USA.
authors.forename: Jun
authors.initials: J
authors.lastname: Yang
authors.affiliation: Department of Food Science, University of Massachusetts, Amherst, MA, 01003, USA.
authors.forename: Haixia
authors.initials: H
authors.lastname: Yang
authors.affiliation: Department of Food Science, University of Massachusetts, Amherst, MA, 01003, USA; Molecular and Cellular Biology Graduate Program, University of Massachusetts, Amherst, MA, 01003, USA.
authors.forename: Katherine Z
authors.initials: KZ
authors.lastname: Sanidad
authors.affiliation: Department of Entomology and Comprehensive Cancer Center, University of California, Davis, CA, 95616, USA.
authors.forename: Bruce D
authors.initials: BD
authors.lastname: Hammock
authors.affiliation: Department of Mathematics and Statistics, University of Massachusetts, Amherst, MA, 01003, USA.
authors.forename: Daeyoung
authors.initials: D
authors.lastname: Kim
authors.affiliation: Department of Food Science, University of Massachusetts, Amherst, MA, 01003, USA; Molecular and Cellular Biology Graduate Program, University of Massachusetts, Amherst, MA, 01003, USA. Electronic address: guodongzhang@umass.edu.
authors.forename: Guodong
authors.initials: G
authors.lastname: Zhang
mesh_terms: D000818:Animals; D059305:Diet, High-Fat / Q000009:adverse effects*; D015777:Eicosanoids / Q000097:blood* / Q000378:metabolism*; D004798:Enzymes / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D055432:Metabolomics; D051379:Mice; D008810:Mice, Inbred C57BL
chemical_list: D015777:Eicosanoids; D004798:Enzymes
keywords: Eicosanoids; Epoxyoctadecenoic acids; High-fat diet; Leukotoxins; Obesity