title: Early effects of continuous positive airway pressure in a rodent model of allergic rhinitis.
abstract: Continuous positive airway pressure (CPAP) is the most commonly used treatment in obstructive sleep apnea. In a previous rat model study, we demonstrated that nasal CPAP induces early rhinitis expressed by nasal neutrophil extravasation. Here we hypothesized that nasal CPAP would worsen nasal inflammation on a previously inflamed mucosa. The objective of this study was to evaluate the early nasal CPAP effects of allergic rhinitis (AR) in a rodent model.
  
  
  
  Twenty Sprague-Dawley rats were sensitized with intraperitoneal ovalbumin (OVA). Nasal inflammation was induced by the administration of intranasal OVA during consecutive days. The same procedure was performed in 20 control rats treated with saline solution. The allergic (AR) and non-allergic (NAR) rats were then randomized to nasal CPAP at 10 cm H2O for five hours or to sham CPAP. The degree of nasal inflammation was assessed by evaluating the percentage of neutrophils, eosinophils, basophils, and lymphocytes in the nasal mucosa. An unpaired Mann-Whitney test was used to analyze differences between groups.
  
  
  
  The greatest inflammation was observed in the group of AR without CPAP (1.24% ± 0.94%), followed by NAR with CPAP (0.64% ± 0.30%), AR with CPAP (0.64% ± 0.40%), and NAR without CPAP (0.21% ± 0.29%).
  
  
  
  Administration of nasal CPAP or allergy sensitization can produce, individually, neutrophil extravasation on the nasal mucosa of a rat model. The application of both stimuli is not responsible for increased inflammation. Therefore, this study suggests that rhinitis is not a major limitation for CPAP administration.
authors.affiliation: Sleep Disorders Unit Hospital Clínic, Barcelona, Spain; Ciber Enfermedades Respiratorias (CIBERES), Bunyola, Spain; Faculty of Medicine, University of Barcelona, Barcelona, Spain. Electronic address: ivila@clinic.ub.es.
authors.forename: I
authors.initials: I
authors.lastname: Vilaseca
authors.affiliation: Otolaryngology Department, Hospital Esperit Sant, Santa Coloma de Gramanet, Spain.
authors.forename: E
authors.initials: E
authors.lastname: Lehrer-Coriat
authors.affiliation: Ciber Enfermedades Respiratorias (CIBERES), Bunyola, Spain.
authors.forename: M
authors.initials: M
authors.lastname: Torres
authors.affiliation: Otolaryngology Department, Hospital General de Granollers, Granollers, Spain.
authors.forename: F
authors.initials: F
authors.lastname: Aguilar
authors.affiliation: Biophysics and Bioengineering Unit, Medicine Faculty, University of Barcelona, Barcelona, Spain.
authors.forename: I
authors.initials: I
authors.lastname: Almendros
authors.affiliation: Otolaryngology Department, Hospital del Sagrat Cor, Barcelona, Spain.
authors.forename: B M
authors.initials: BM
authors.lastname: Martínez-Vidal
authors.affiliation: Ciber Enfermedades Respiratorias (CIBERES), Bunyola, Spain; Otolaryngology Department, Hospital Esperit Sant, Santa Coloma de Gramanet, Spain; Institut d' Investigació Biomèdica August Pi i Sunyer (IDIBAPS), Barcelona, Spain.
authors.forename: R
authors.initials: R
authors.lastname: Farré
authors.affiliation: Sleep Disorders Unit Hospital Clínic, Barcelona, Spain; Ciber Enfermedades Respiratorias (CIBERES), Bunyola, Spain; Faculty of Medicine, University of Barcelona, Barcelona, Spain; Biophysics and Bioengineering Unit, Medicine Faculty, University of Barcelona, Barcelona, Spain; Institut d' Investigació Biomèdica August Pi i Sunyer (IDIBAPS), Barcelona, Spain.
authors.forename: J M
authors.initials: JM
authors.lastname: Montserrat
mesh_terms: D000281:Administration, Intranasal; D000818:Animals; D045422:Continuous Positive Airway Pressure / Q000379:methods; D004195:Disease Models, Animal; D009297:Nasal Mucosa / Q000276:immunology* / Q000473:pathology*; D010047:Ovalbumin; D011897:Random Allocation; D017207:Rats, Sprague-Dawley; D065631:Rhinitis, Allergic / Q000276:immunology / Q000473:pathology / Q000628:therapy*; D013997:Time Factors
chemical_list: D010047:Ovalbumin
keywords: Allergic rhinitis; CPAP; CPAP-induced rhinitis; Nasal inflammation