title: Regulation of Contractile Responses of Vascular Smooth Muscle Cells under Conditions of Hypoxia-Reoxygenation.
abstract: We analyzed the effects of hypoxia and reoxygenation on changes in contractile activity in rat aortic smooth muscles. Both hypoxia and reoxygenation induced relaxation of smooth muscle cells precontracted with high-potassium Krebs solution (30 mM KCl) or α1-adrenoceptor agonist phenylephrine. Vasodilation resulted from enhancement of potassium permeability of smooth muscle cell membranes caused by activation of voltage-gated potassium channels (triggered by both precontracting agents) or by opening of ATP-sensitive potassium channels (phenylephrine). In isolated smooth muscle cells, both hypoxia and inhibition of Na+,K+-ATPase with ouabain led to depletion of intracellular store of macroergic substances, reduced potassium concentration, and elevated the content of sodium ions.
authors.affiliation: Siberian State Medical University, Ministry of Health of the Russian Federation, Tomsk, Russia. gusacova@yandex.ru.
authors.forename: S V
authors.initials: SV
authors.lastname: Gusakova
authors.affiliation: Siberian State Medical University, Ministry of Health of the Russian Federation, Tomsk, Russia.
authors.forename: Yu G
authors.initials: YG
authors.lastname: Birulina
authors.affiliation: Siberian State Medical University, Ministry of Health of the Russian Federation, Tomsk, Russia.|National Research Tomsk Polytechnic University, Tomsk, Russia.
authors.forename: L V
authors.initials: LV
authors.lastname: Smagliy
authors.affiliation: Siberian State Medical University, Ministry of Health of the Russian Federation, Tomsk, Russia.
authors.forename: I V
authors.initials: IV
authors.lastname: Kovalev
authors.affiliation: Siberian State Medical University, Ministry of Health of the Russian Federation, Tomsk, Russia.
authors.forename: I V
authors.initials: IV
authors.lastname: Petrova
authors.affiliation: Siberian State Medical University, Ministry of Health of the Russian Federation, Tomsk, Russia.|National Research Tomsk Polytechnic University, Tomsk, Russia.
authors.forename: A V
authors.initials: AV
authors.lastname: Nosarev
authors.affiliation: National Research Tomsk Polytechnic University, Tomsk, Russia.
authors.forename: S N
authors.initials: SN
authors.lastname: Orlov
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D058646:Adrenergic alpha-1 Receptor Agonists / Q000494:pharmacology; D000818:Animals; D001011:Aorta / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D004791:Enzyme Inhibitors / Q000494:pharmacology; D000860:Hypoxia / Q000378:metabolism*; D007537:Isometric Contraction / Q000187:drug effects*; D054086:KATP Channels / Q000378:metabolism; D008297:Male; D009131:Muscle, Smooth, Vascular / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D032389:Myocytes, Smooth Muscle / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D010042:Ouabain / Q000494:pharmacology; D010100:Oxygen / Q000494:pharmacology*; D010656:Phenylephrine / Q000494:pharmacology; D011188:Potassium / Q000378:metabolism*; D024642:Potassium Channels, Voltage-Gated / Q000378:metabolism; D061251:Primary Cell Culture; D051381:Rats; D017208:Rats, Wistar; D012964:Sodium / Q000378:metabolism*; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism; D046509:Tissue Culture Techniques; D014664:Vasodilation / Q000187:drug effects
chemical_list: D058646:Adrenergic alpha-1 Receptor Agonists; D004791:Enzyme Inhibitors; D054086:KATP Channels; D024642:Potassium Channels, Voltage-Gated; D010656:Phenylephrine; D010042:Ouabain; D000255:Adenosine Triphosphate; D012964:Sodium; D000254:Sodium-Potassium-Exchanging ATPase; D011188:Potassium; D010100:Oxygen
keywords: hypoxia; rat aorta; reoxygenation, Na+,K+-ATPase; smooth muscle cells