title: Degenerative myelopathy in the Collie breed: a retrospective immunohistochemical analysis of superoxide dismutase 1 in an affected Rough Collie, and a molecular epidemiological survey of the SOD1: c.118G>A mutation in Japan.
abstract: Canine degenerative myelopathy (DM) is an adult-onset, progressive neurodegenerative disease that occurs in multiple dog breeds. A DM-associated mutation of the canine superoxide dismutase 1 (SOD1) gene, designated as c.118G>A (p.E40K), has been implicated as one of pathogenetic determinants of the disease in many breeds, but it remains to be determined whether the c.118G>A mutation is responsible for development or progression of DM in Collies. Previously, a Rough Collie was diagnosed clinically and histopathologically as having DM in Japan, suggesting the possibility that the Collie breed may be predisposed to DM due to the high frequency of c.118G>A in Japan. In this study, accumulation and aggregate formation of SOD1 protein were retrospectively demonstrated in the spinal cord of the DM-affected dog by immunohistochemical analysis. Furthermore, a molecular epidemiological survey revealed a high carrier rate (27.6%) and mutant allele frequency (0.138) of c.118G>A in a population of Collies in Japan, suggesting that the Collie breed may be predisposed to DM associated with c.118G>A, and the prevention of DM in Collies in Japan should be addressed through epidemiological and genetic testing strategies.
authors.affiliation: Laboratory of Clinical Pathology, Joint Faculty of Veterinary Medicine, Kagoshima University, 1-21-24 Kohrimoto, Kagoshima 890-0065, Japan.
authors.forename: Moeko
authors.initials: M
authors.lastname: Kohyama
authors.forename: Masato
authors.initials: M
authors.lastname: Kitagawa
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Kamishina
authors.forename: Yui
authors.initials: Y
authors.lastname: Kobatake
authors.forename: Akira
authors.initials: A
authors.lastname: Yabuki
authors.forename: Mariko
authors.initials: M
authors.lastname: Sawa
authors.forename: Shusaku
authors.initials: S
authors.lastname: Kakita
authors.forename: Osamu
authors.initials: O
authors.lastname: Yamato
mesh_terms: D000818:Animals; D004283:Dog Diseases / Q000201:enzymology / Q000235:genetics* / Q000473:pathology; D004285:Dogs; D020022:Genetic Predisposition to Disease; D007150:Immunohistochemistry / Q000662:veterinary; D007564:Japan; D008297:Male; D019636:Neurodegenerative Diseases / Q000201:enzymology / Q000235:genetics / Q000473:pathology / Q000662:veterinary*; D017354:Point Mutation; D012189:Retrospective Studies; D013045:Species Specificity; D013118:Spinal Cord Diseases / Q000201:enzymology / Q000235:genetics / Q000473:pathology / Q000662:veterinary*; D000072105:Superoxide Dismutase-1 / Q000235:genetics*
chemical_list: D000072105:Superoxide Dismutase-1