title: A high yield DNA extraction method for medically importantCandidaspecies: A comparison of manual versus QIAcube-based automated system.
abstract: The prognosis of infected individuals with candidemia depends on rapid and precise diagnosis which enables optimising treatment. Three fungal DNA extraction protocols have been compared in this study for medically important Candida species. The quality and quantity of the DNA extracted by physical, chemical and automated protocols was compared using NanoDrop ND-2000 spectrophotometer. It was found that the yield and purity (260/230) ratio of extracted DNA was significantly high in the physical treatment-based protocol as compared to chemical based or automated protocol. Extracted DNA-based real time-polymerase chain reaction showed an analytical sensitivity of 103 cfu/mL. The result of this study suggests physical treatment is the most successful extraction technique compared to other two protocols.
authors.affiliation: Department of Microbiology, Tata Medical Center, Kolkata, West Bengal, India.
authors.forename: P
authors.initials: P
authors.lastname: Das
authors.affiliation: Department of Microbiology, Tata Medical Center, Kolkata, West Bengal, India.
authors.forename: P
authors.initials: P
authors.lastname: Pandey
authors.affiliation: Department of Microbiology, Tata Medical Center, Kolkata, West Bengal, India.
authors.forename: A
authors.initials: A
authors.lastname: Harishankar
authors.affiliation: Department of Microbiology, Tata Medical Center, Kolkata, West Bengal, India.
authors.forename: M
authors.initials: M
authors.lastname: Chandy
authors.affiliation: Department of Microbiology, Tata Medical Center, Kolkata, West Bengal, India.
authors.forename: S
authors.initials: S
authors.lastname: Bhattacharya
mesh_terms: D057205:Automation, Laboratory / Q000379:methods*; D002175:Candida / Q000235:genetics*; D058387:Candidemia / Q000175:diagnosis; D004247:DNA / Q000302:isolation & purification*; D006801:Humans; D008828:Microbiological Techniques / Q000379:methods*; D013048:Specimen Handling / Q000379:methods*
chemical_list: D004247:DNA