title: CHARACTERISTICS AND FERTILITY OF SUMATRAN TIGER SPERMATOZOA CRYOPRESERVED WITH DIFFERENT SUGARS.
abstract: Cryopreservation of semen is one of the most important methods for the preservation of endangered tigers.
  
  
  
  This study evaluated the effects of sugar supplementation on the cryosurvival of spermatozoa from Sumatran tigers (Panthera tigris sumatrae).
  
  
  
  The post-thaw characteristics and fertility of spermatozoa cryopreserved with different sugars (glucose, lactose, and trehalose) were evaluated using heterologous in-vitro fertilisation with cat oocytes.
  
  
  
  All parameters of post-thaw spermatozoa significantly decreased as compared with those of fresh spermatozoa. The index of sperm motility for semen cryopreserved with lactose was significantly higher than that for semen cryopreserved with trehalose. The percentage of total fertilisation for tiger spermatozoa cryopreserved with trehalose was significantly lower than that for control cat spermatozoa.
  
  
  
  Our findings indicated that supplementation with lactose or glycerol as the main sugar in the egg yolk extender resulted in a better motility and fertility potential for post-thawed spermatozoa.
authors.affiliation: Faculty of Veterinary Science, Bogor Agricultural University, Indonesia.
authors.forename: Ni
authors.initials: N
authors.lastname: Wayan Kurniani Karja
authors.affiliation: Faculty of Veterinary Science, Bogor Agricultural University, Indonesia.
authors.forename: Mokhamad
authors.initials: M
authors.lastname: Fahrudin
authors.affiliation: Faculty of Veterinary Science, Bogor Agricultural University, Indonesia.
authors.forename: Mohamad Agus
authors.initials: MA
authors.lastname: Setiadi
authors.affiliation: Faculty of Veterinary Science, Bogor Agricultural University; Taman Safari Indonesia, Bogor, Indonesia.
authors.forename: Ligaya Ita
authors.initials: LI
authors.lastname: Tumbelaka
authors.affiliation: Taman Safari Indonesia, Bogor, Indonesia.
authors.forename: Retno
authors.initials: R
authors.lastname: Sudarwati
authors.affiliation: Taman Safari Indonesia, Bogor, Indonesia.
authors.forename: Yohana Tri
authors.initials: YT
authors.lastname: Hastuti
authors.affiliation: Taman Safari Indonesia, Bogor, Indonesia.
authors.forename: Bongot Huas
authors.initials: BH
authors.lastname: Mulia
authors.affiliation: Taman Safari Indonesia, Bogor, Indonesia.
authors.forename: Ardyta
authors.initials: A
authors.lastname: Widianti
authors.affiliation: Taman Safari Indonesia, Bogor, Indonesia.
authors.forename: Keni
authors.initials: K
authors.lastname: Sultan
authors.affiliation: The United Graduate School of Veterinary Science, Yamaguchi University, Yamaguchi, Japan.
authors.forename: Tsukasa
authors.initials: T
authors.lastname: Terazono
authors.affiliation: The United Graduate School of Veterinary Science, Yamaguchi University, Yamaguchi, Japan.
authors.forename: Zhao
authors.initials: Z
authors.lastname: Namula
authors.affiliation: The United Graduate School of Veterinary Science, Yamaguchi University, Yamaguchi, Japan.
authors.forename: Masayasu
authors.initials: M
authors.lastname: Taniguchi
authors.affiliation: The United Graduate School of Veterinary Science, Yamaguchi University, Yamaguchi,; Faculty of Bioscience and Bioindustry, Tokushima University, Tokushima, Japan.
authors.forename: Fuminori
authors.initials: F
authors.lastname: Tanihara
authors.affiliation: Fujii Memorial Institute of Medical Sciences, Tokushima University, Tokushima, Japan.
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Takemoto
authors.affiliation: Department of Animal Science, National Institute of Agrobiological Sciences, Ibaraki, Japan.
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Kikuchi
authors.affiliation: The United Graduate School of Veterinary Science, Yamaguchi University, Yamaguchi, Japan. pxx13340@nifty.ne.jp.
authors.forename: Yoko
authors.initials: Y
authors.lastname: Sato
authors.affiliation: The United Graduate School of Veterinary Science, Yamaguchi University, Yamaguchi; Faculty of Bioscience and Bioindustry, Tokushima University, Tokushima, Japan.
authors.forename: Takeshige
authors.initials: T
authors.lastname: Otoi
mesh_terms: D000818:Animals; D015925:Cryopreservation / Q000379:methods*; D003451:Cryoprotective Agents / Q000494:pharmacology*; D005298:Fertility / Q000187:drug effects*; D005947:Glucose / Q000494:pharmacology; D007785:Lactose / Q000494:pharmacology; D008297:Male; D012662:Semen Preservation / Q000379:methods*; D013094:Spermatozoa / Q000187:drug effects*; D046010:Tigers; D014199:Trehalose / Q000494:pharmacology
chemical_list: D003451:Cryoprotective Agents; D014199:Trehalose; D005947:Glucose; D007785:Lactose