title: Dual-purpose corneal tissue for anterior lamellar keratoplasty and Descemet's membrane endothelial keratoplasty.
abstract: OBJECTIVE
  To assess the intraoperative issues and surgical outcomes of preparing a single-donor corneal tissue for same-day use in both deep anterior lamellar keratoplasty (DALK) and Descemet's membrane endothelial keratoplasty (DMEK).
  
  
  METHODS
  Consecutive retrospective case series.
  
  
  METHODS
  Ten eyes of 10 patients who underwent DALK (5 patients) or DMEK (5 patients) surgery using dual-purpose corneal tissue.
  
  
  METHODS
  Five dual-purpose corneoscleral rims were used to prepare tissue for 5 DMEK and 5 DALK procedures. The submerged cornea using backgrounds away technique was first used to harvest the 5 DMEK grafts, and the remaining tissue was used for the 5 DALK grafts. Tissue preparation and operative use occurred on the same day. Tissue preparation challenges, intraoperative complications, and visual recovery were assessed.
  
  
  RESULTS
  There were no difficulties in preparing the 5 dual-purpose tissues, and all 10 lamellar transplants were completed successfully. At the 6-month follow-up, the mean best-corrected distance visual acuity improved from 20/250 to 20/80 in the DALK patients, and from 20/300 to 20/25 in the DMEK patients. Postoperative complications after DALK included retained viscoelastic agent at the interface in 1 patient and a double anterior chamber managed with rebubbling in another. After DMEK, a peripheral partial graft detachment occurred in 1 patient and was managed successfully with rebubbling. All corneas demonstrated clarity on slit-lamp examination.
  
  
  CONCLUSIONS
  Single-donor corneal tissue can be effectively used for both DALK and DMEK, and may represent a more efficient use of corneal tissue. Complications with the preparation of dual-purpose tissue were not encountered.
authors.affiliation: Ottawa Hospital Research Institute, Regenerative Medicine Program, Vision Research Centre, Ottawa, Canada; Faculty of Medicine, Department of Ophthalmology, University of Ottawa, Ottawa, Canada, Ottawa, Canada.
authors.forename: C Larena
authors.initials: CL
authors.lastname: Menant-Tay
authors.affiliation: Ottawa Hospital Research Institute, Regenerative Medicine Program, Vision Research Centre, Ottawa, Canada; Faculty of Medicine, Department of Ophthalmology, University of Ottawa, Ottawa, Canada, Ottawa, Canada.
authors.forename: Ronan
authors.initials: R
authors.lastname: Conlon
authors.affiliation: Faculty of Medicine, Department of Ophthalmology, University of British Columbia, Vancouver, B.C.
authors.forename: Salina
authors.initials: S
authors.lastname: Teja
authors.affiliation: Ottawa Hospital Research Institute, Regenerative Medicine Program, Vision Research Centre, Ottawa, Canada; Faculty of Medicine, Department of Ophthalmology, University of Ottawa, Ottawa, Canada, Ottawa, Canada.
authors.forename: Season
authors.initials: S
authors.lastname: Yeung
authors.affiliation: Ottawa Hospital Research Institute, Regenerative Medicine Program, Vision Research Centre, Ottawa, Canada; Faculty of Medicine, Department of Ophthalmology, University of Ottawa, Ottawa, Canada, Ottawa, Canada.
authors.forename: Joshua
authors.initials: J
authors.lastname: Teichman
authors.affiliation: Ottawa Hospital Research Institute, Regenerative Medicine Program, Vision Research Centre, Ottawa, Canada; Faculty of Medicine, Department of Ophthalmology, University of Ottawa, Ottawa, Canada, Ottawa, Canada.
authors.forename: Setareh
authors.initials: S
authors.lastname: Ziai
authors.affiliation: Ottawa Hospital Research Institute, Regenerative Medicine Program, Vision Research Centre, Ottawa, Canada; Faculty of Medicine, Department of Ophthalmology, University of Ottawa, Ottawa, Canada, Ottawa, Canada. Electronic address: baig247@gmail.com.
authors.forename: Kashif
authors.initials: K
authors.lastname: Baig
mesh_terms: D000368:Aged; D002170:Canada; D003315:Cornea; D003316:Corneal Diseases / Q000601:surgery; D016039:Corneal Transplantation / Q000379:methods*; D057111:Descemet Stripping Endothelial Keratoplasty / Q000379:methods*; D046148:Donor Selection; D005260:Female; D006801:Humans; D007431:Intraoperative Complications; D008297:Male; D008875:Middle Aged; D011183:Postoperative Complications; D012189:Retrospective Studies; D013997:Time Factors; D014019:Tissue Donors; D009927:Tissue and Organ Procurement; D014792:Visual Acuity