title: Structural and functional study of ChuY from Escherichia coli strain CFT073.
abstract: The uropathogenic Escherichia coli strain CFT073 contains multiple iron and heme transport systems, which facilitate infection of the host urinary tract. To elucidate the molecular and cellular function of ChuY, a hypothetical gene in the heme degradation/utilization pathway, we solved the crystal structure of ChuY at 2.4 Å resolution. ChuY has high structural homology with human biliverdin and flavin reductase. We confirmed that ChuY has flavin mononucleotide (FMN) reductase activity, using NAD(P)H as a cofactor, and shows porphyrin ring binding affinity. A chuY deletion-insertion strain showed reduced survival potential compared to wild-type and complemented strains in mammalian cells. Current results suggest ChuY acts as a reductase in heme homeostasis to maintain the virulence potential of E. coli CFT073.
authors.affiliation: Department of Molecular Cell Biology, Sungkyunkwan University School of Medicine, Suwon, Gyeonggi 16419, South Korea.
authors.forename: Hun
authors.initials: H
authors.lastname: Kim
authors.affiliation: Department of Molecular Cell Biology, Sungkyunkwan University School of Medicine, Suwon, Gyeonggi 16419, South Korea.
authors.forename: Akhilesh Kumar
authors.initials: AK
authors.lastname: Chaurasia
authors.affiliation: Department of Molecular Cell Biology, Sungkyunkwan University School of Medicine, Suwon, Gyeonggi 16419, South Korea.
authors.forename: Truc
authors.initials: T
authors.lastname: Kim
authors.affiliation: Department of Cosmetic Science, Chungwoon University, Hongseong, Chungnam 32244, South Korea.
authors.forename: Jongkeun
authors.initials: J
authors.lastname: Choi
authors.affiliation: Pohang Accelerator Laboratory, Pohang, Gyeongbuk 37673, South Korea.
authors.forename: Sung Chul
authors.initials: SC
authors.lastname: Ha
authors.affiliation: Department of Molecular Cell Biology, Sungkyunkwan University School of Medicine, Suwon, Gyeonggi 16419, South Korea.
authors.forename: Doyoun
authors.initials: D
authors.lastname: Kim
authors.affiliation: Department of Molecular Cell Biology, Sungkyunkwan University School of Medicine, Suwon, Gyeonggi 16419, South Korea. Electronic address: kyeongkyu@skku.edu.
authors.forename: Kyeong Kyu
authors.initials: KK
authors.lastname: Kim
mesh_terms: D000818:Animals; D001664:Biliverdine / Q000737:chemistry; D018360:Crystallography, X-Ray; D004926:Escherichia coli / Q000378:metabolism* / Q000472:pathogenicity; D029968:Escherichia coli Proteins / Q000737:chemistry / Q000378:metabolism*; D038181:FMN Reductase / Q000737:chemistry; D017353:Gene Deletion; D023281:Genomics; D057809:HEK293 Cells; D006418:Heme / Q000737:chemistry; D006427:Hemin / Q000737:chemistry; D006706:Homeostasis; D006801:Humans; D007501:Iron / Q000737:chemistry; D051379:Mice; D009249:NADP / Q000737:chemistry; D044925:Oxidoreductases Acting on CH-CH Group Donors / Q000378:metabolism*; D011166:Porphyrins / Q000737:chemistry; D011487:Protein Conformation; D017433:Protein Structure, Secondary; D000067996:RAW 264.7 Cells; D014774:Virulence
chemical_list: D029968:Escherichia coli Proteins; D011166:Porphyrins; D006418:Heme; D009249:NADP; D006427:Hemin; D007501:Iron; C000618859:ChuY protein, E coli; D044925:Oxidoreductases Acting on CH-CH Group Donors; D038181:FMN Reductase; C540768:Fre protein, E coli; D001664:Biliverdine
keywords: Crystal structure; Flavin reductase; Heme/iron homeostasis; Hypothetical protein; Uropathogenic E. coli CFT073; chuY