title: Fast and sensitive analysis of beta blockers by ultra-high-performance liquid chromatography coupled with ultra-high-resolution TOF mass spectrometry.
abstract: This paper presents a method for the determination of acebutolol, betaxolol, bisoprolol, metoprolol, nebivolol and sotalol in human serum by liquid-liquid extraction and ultra-high-performance liquid chromatography coupled with ultra-high-resolution TOF mass spectrometry. After liquid-liquid extraction, beta blockers were separated on a reverse-phase analytical column (Acclaim RS 120; 100 × 2.1 mm, 2.2 μm). The total run time was 6 min for each sample. Linearity, limit of detection, limit of quantification, matrix effects, specificity, precision, accuracy, recovery and sample stability were evaluated. The method was successfully applied to the therapeutic drug monitoring of 108 patients with hypertension. This method was also used for determination of beta blockers in 33 intoxicated patients.
authors.affiliation: Faculty of Medicine and Dentistry, Palacky University, Olomouc, Czech Republic.|Department of Forensic Medicine and Medical Law, University Hospital Olomouc, Olomouc, Czech Republic.
authors.forename: Jana
authors.initials: J
authors.lastname: Tomková
authors.affiliation: Faculty of Medicine and Dentistry, Palacky University, Olomouc, Czech Republic.|Department of Forensic Medicine and Medical Law, University Hospital Olomouc, Olomouc, Czech Republic.
authors.forename: Peter
authors.initials: P
authors.lastname: Ondra
authors.affiliation: Department of Forensic Medicine and Medical Law, University Hospital Olomouc, Olomouc, Czech Republic.|Department of Internal Medicine I - Cardiology, Faculty of Medicine and Dentistry, Palacky University Olomouc, Olomouc, Czech Republic.
authors.forename: Eva
authors.initials: E
authors.lastname: Kocianová
authors.affiliation: Department of Forensic Medicine and Medical Law, University Hospital Olomouc, Olomouc, Czech Republic.|Department of Internal Medicine I - Cardiology, Faculty of Medicine and Dentistry, Palacky University Olomouc, Olomouc, Czech Republic.
authors.forename: Jan
authors.initials: J
authors.lastname: Václavík
mesh_terms: D000319:Adrenergic beta-Antagonists / Q000097:blood*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005260:Female; D006801:Humans; D057230:Limit of Detection; D008297:Male; D013058:Mass Spectrometry / Q000379:methods*; D008875:Middle Aged; D012015:Reference Standards; D015203:Reproducibility of Results
chemical_list: D000319:Adrenergic beta-Antagonists
keywords: beta blockers; forensic toxicological screening; liquid chromatography; mass spectrometry; therapeutic drug monitoring