title: Multiplex Detection of Toxigenic Penicillium Species.
abstract: Multiplex PCR-based methods for simultaneous detection and quantification of different mycotoxin-producing Penicillia are useful tools to be used in food safety programs. These rapid and sensitive techniques allow taking corrective actions during food processing or storage for avoiding accumulation of mycotoxins in them. In this chapter, three multiplex PCR-based methods to detect at least patulin- and ochratoxin A-producing Penicillia are detailed. Two of them are different multiplex real-time PCR suitable for monitoring and quantifying toxigenic Penicillium using the nonspecific dye SYBR Green and specific hydrolysis probes (TaqMan). All of them successfully use the same target genes involved in the biosynthesis of such mycotoxins for designing primers and/or probes.
authors.affiliation: Faculty of Veterinary Science, Food Hygiene and Safety, Meat and Meat Products Research Institute, University of Extremadura, Avda de las Ciencias s/n, 10003, Cáceres, Spain. aliciarj@unex.es.
authors.forename: Alicia
authors.initials: A
authors.lastname: Rodríguez
authors.affiliation: Faculty of Veterinary Science, Food Hygiene and Safety, Meat and Meat Products Research Institute, University of Extremadura, Avda de las Ciencias s/n, 10003, Cáceres, Spain.
authors.forename: Juan J
authors.initials: JJ
authors.lastname: Córdoba
authors.affiliation: Faculty of Veterinary Science, Food Hygiene and Safety, Meat and Meat Products Research Institute, University of Extremadura, Avda de las Ciencias s/n, 10003, Cáceres, Spain.
authors.forename: Mar
authors.initials: M
authors.lastname: Rodríguez
authors.affiliation: Faculty of Veterinary Science, Food Hygiene and Safety, Meat and Meat Products Research Institute, University of Extremadura, Avda de las Ciencias s/n, 10003, Cáceres, Spain.
authors.forename: María J
authors.initials: MJ
authors.lastname: Andrade
mesh_terms: D060885:Multiplex Polymerase Chain Reaction; D009183:Mycotoxins / Q000096:biosynthesis / Q000235:genetics*; D009793:Ochratoxins / Q000096:biosynthesis; D010365:Patulin / Q000096:biosynthesis / Q000235:genetics; D010407:Penicillium / Q000145:classification* / Q000235:genetics* / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction
chemical_list: D009183:Mycotoxins; D009793:Ochratoxins; C025589:ochratoxin A; D010365:Patulin
keywords: Multiplex; Ochratoxin A; PCR; Patulin; Penicillium; idh; otanpsPN; qPCR