title: [Contribution of nested PCR in the diagnosis of imported malaria in southern Algeria].
abstract: The nested PCR was used to estimate its inputs in malaria diagnosis and in the performance of the microscope operators involved in the surveillance of malaria in remote areas of South Algeria. For the period 2010 to 2015, 112 patients (93 febrile and 19 asymptomatic) coming from sub-Saharan Africa were tested for malaria in the hospital of Tamanrasset. One part of the blood taken from fingertip was used for blood smears and the second part was absorbed in filter paper for molecular diagnosis. Overall, the infection was detected by nested PCR in 63 samples versus 53 by direct examination. In addition, 11 mixed infections and 6 positive asymptomatic cases not detected by microscopy were diagnosed by PCR. Moreover, two negative samples in nested PCR were tested positive by direct examination. The molecular tool is more sensitive than the direct examination in detecting infra-microscopic parasitaemia and mixed infections...
authors.affiliation: Laboratoire d'éco-épidémiologie parasitaire et génétique des populations., Institut Pasteur d'Algérie, Route du petit Staoueli, Dely-Ibrahim Alger, Algérie. lazhari_bouiba@hotmail.com.
authors.forename: L
authors.initials: L
authors.lastname: Bouiba
authors.affiliation: Service d'épidémiologie et de médecine préventive, établissement publique hospitalier de Tamanrasset, Tamanrasset, Algérie.
authors.forename: B
authors.initials: B
authors.lastname: Gassen
authors.affiliation: Laboratoire de référence national du paludisme, Institut national de santé publique, El Biar, Algérie.
authors.forename: M
authors.initials: M
authors.lastname: Gasmi
authors.affiliation: Laboratoire de référence national du paludisme, Institut national de santé publique, El Biar, Algérie.
authors.forename: D
authors.initials: D
authors.lastname: Hammadi
authors.affiliation: Laboratoire d'éco-épidémiologie parasitaire et génétique des populations., Institut Pasteur d'Algérie, Route du petit Staoueli, Dely-Ibrahim Alger, Algérie.
authors.forename: Z
authors.initials: Z
authors.lastname: Harrat
mesh_terms: D017954:Africa South of the Sahara; D000462:Algeria / Q000453:epidemiology; D016054:DNA, Protozoan / Q000032:analysis; D054242:Emigrants and Immigrants / Q000706:statistics & numerical data; D006801:Humans; D008288:Malaria / Q000175:diagnosis* / Q000453:epidemiology / Q000469:parasitology*; D010963:Plasmodium falciparum / Q000235:genetics*; D010966:Plasmodium vivax / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods*; D011237:Predictive Value of Tests; D012680:Sensitivity and Specificity
chemical_list: D016054:DNA, Protozoan
keywords: Algeria; Imported malaria; Mixed infections; Nested PCR; Sub-Saharan Africa; Tamanrasset; Thin and thick smear