title: A tomato plastidic ATP/ADP transporter geneSlAATPincreases starch content in transgenicArabidopsis.
abstract: A plastidic ATP/ADP transporter (AATP) is responsible for importing ATP from the cytosol into plastids. Increasing the ATP supply is a potential way to facilitate anabolic synthesis in heterotrophic plastids of plants. In this work, a gene encoding the AATP protein, namedSlAATP, was successfully isolated from tomato. Expression ofSlAATPwas induced by exogenous sucrose treatment in tomato. The coding region ofSlAATPwas cloned into a binary vector under the control of 35S promoter and then transformed intoArabidopsisto obtain transgenic plants. Constitutive expression ofSlAATPsignificantly increased the starch accumulation in the transgenic plants. Real-time quantitative PCR (qRT-PCR) analysis showed that constitutive expression ofStAATPup-regulated the expression of phosphoglucomutase (AtPGM), ADP-glucose pyrophosphorylase (AtAGPase), granule-bound starch synthase (AtGBSS IandAtGBSS II), soluble starch synthases (AtSSS I,AtSSS II,AtSSS IIIandAtSSS IV) and starch branching enzyme (AtSBE IandAtSBE II) genes involved in starch biosynthesis in the transgenicArabidopsisplants. Meanwhile, enzymatic analyses indicated that the major enzymes (AGPase, GBSS, SSS and SBE) involved in the starch biosynthesis exhibited higher activities in the transgenic plants compared to the wild-type (WT). These findings suggest thatSlAATPmay improve starch content ofArabidopsisby up-regulating the expression of the related genes and increasing the activities of the major enzymes invovled in starch biosynthesis. The manipulation ofSlAATPexpression might be used for increasing starch accumulation of plants in the future.
authors.affiliation: School of Life Science and Food Engineering, Huaiyin Institute of Technology, Huai'an, 223003 Jiangsu China.
authors.forename: Feibing
authors.initials: F
authors.lastname: Wang
authors.affiliation: School of Life Science and Food Engineering, Huaiyin Institute of Technology, Huai'an, 223003 Jiangsu China.
authors.forename: Yuxiu
authors.initials: Y
authors.lastname: Ye
authors.affiliation: School of Life Science and Food Engineering, Huaiyin Institute of Technology, Huai'an, 223003 Jiangsu China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Niu
authors.affiliation: School of Life Science and Food Engineering, Huaiyin Institute of Technology, Huai'an, 223003 Jiangsu China.
authors.forename: Faxiang
authors.initials: F
authors.lastname: Wan
authors.affiliation: School of Life Science and Food Engineering, Huaiyin Institute of Technology, Huai'an, 223003 Jiangsu China.
authors.forename: Bo
authors.initials: B
authors.lastname: Qi
authors.affiliation: School of Life Science and Food Engineering, Huaiyin Institute of Technology, Huai'an, 223003 Jiangsu China.
authors.forename: Xinhong
authors.initials: X
authors.lastname: Chen
authors.affiliation: School of Life Science and Food Engineering, Huaiyin Institute of Technology, Huai'an, 223003 Jiangsu China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Zhou
authors.affiliation: School of Life Science and Food Engineering, Huaiyin Institute of Technology, Huai'an, 223003 Jiangsu China.
authors.forename: Boqing
authors.initials: B
authors.lastname: Chen
keywords: Arabidopsis; Constitutive expression; SlAATP; Starch content; Tomato