title: Pterostilbene protects against myocardial ischemia/reperfusion injury via suppressing oxidative/nitrative stress and inflammatory response.
abstract: Recent studies have shown that pterostilbene (Pte) confers protection against myocardial ischemia/reperfusion injury. The oxidative/nitrative stress and inflammation induce injury after myocardial ischemia/reperfusion. The present study was designed to evaluate whether treatment with Pte attenuates oxidative/nitrative stress and inflammation in myocardial ischemia/reperfusion (MI/R). Rats were subjected to 30min of myocardial ischemia and 3h of reperfusion, and the rats were administered with vehicle or Pte. The results showed that Pte (10mg/kg) dramatically improved cardiac function and reduced myocardial infarction and myocardial apoptosis following MI/R. As an indicator of oxidative/nitrative stress, myocardial ONOO-content was markedly reduced after Pte treatment. And, Pte led to a dramatic decrease in superoxide generation and malondialdehyde (MDA) content and a dramatic increase in superoxide dismutase (SOD) activity. In addition, Pte treatment significantly reduced p38 MAPK activation and the expression of iNOS and gp91phoxand increased phosphorylated eNOS expression. Pte treatment dramatically decreased myocardial TNF-α, and IL-1β levels and myeloperoxidase (MPO) activity. Furthermore, ONOO-suppression by either Pte or uric acid (UA), an ONOO-scavenger, reduced myocardial injury. In conclusion, Pte exerts a protective effect against MI/R injury by suppressing oxidative/nitrative stress. These results provide evidence that Pte might be a therapeutic approach for the treatment of MI/R injury.
authors.affiliation: Department of Intensive Care Unit, First Affiliated Hospital of Xinjiang Medical University, Urumqi 830054, China.
authors.forename: Zhaoxia
authors.initials: Z
authors.lastname: Yu
authors.affiliation: Department of Neurosurgery, Second Affiliated Hospital of Anhui Medical University, Hefei 230000, China.
authors.forename: Shaohua
authors.initials: S
authors.lastname: Wang
authors.affiliation: Cardiac Center, First Affiliated Hospital of Xinjiang Medical University, Urumqi 830054, China. Electronic address: zhangheart7@163.com.
authors.forename: Xiangyang
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Department of Intensive Care Unit, First Affiliated Hospital of Xinjiang Medical University, Urumqi 830054, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Li
authors.affiliation: Cardiac Center, First Affiliated Hospital of Xinjiang Medical University, Urumqi 830054, China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Zhao
authors.affiliation: Institute of Clinical Medicine Research, First Affiliated Hospital of Xinjiang Medical University, Urumqi 830054, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Liu
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000627:therapeutic use*; D017209:Apoptosis / Q000187:drug effects; D006321:Heart / Q000187:drug effects* / Q000502:physiology; D007249:Inflammation / Q000188:drug therapy*; D053583:Interleukin-1beta / Q000378:metabolism; D008297:Male; D009206:Myocardium / Q000473:pathology*; D018384:Oxidative Stress / Q000187:drug effects; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000188:drug therapy*; D013267:Stilbenes / Q000627:therapeutic use*; D013482:Superoxide Dismutase / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D000893:Anti-Inflammatory Agents; D053583:Interleukin-1beta; D013267:Stilbenes; D014409:Tumor Necrosis Factor-alpha; C107773:pterostilbene; D013482:Superoxide Dismutase
keywords: Inflammation; Myocardial ischemia/reperfusion injury; Oxidative/nitrative stress; Pterostilbene