title: Identification of Potential Biomarkers for Rhegmatogenous Retinal Detachment Associated with Choroidal Detachment by Vitreous iTRAQ-Based Proteomic Profiling.
abstract: Rhegmatogenous retinal detachment associated with choroidal detachment (RRDCD) is a complicated and serious type of rhegmatogenous retinal detachment (RRD). In this study, we identified differentially expressed proteins in the vitreous humors of RRDCD and RRD using isobaric tags for relative and absolute quantitation (iTRAQ) combined with nano-liquid chromatography-electrospray ion trap-mass spectrometry-mass spectrometry (nano-LC-ESI-MS/MS) and bioinformatic analysis. Our result shows that 103 differentially expressed proteins, including 54 up-regulated and 49 down-regulated proteins were identified in RRDCD. Gene ontology (GO) analysis suggested that most of the differentially expressed proteins were extracellular．The Kyoto encyclopedia of genes and genomes (KEGG) pathway analysis suggested that proteins related to complement and coagulation cascades were significantly enriched. iTRAQ-based proteomic profiling reveals that complement and coagulation cascades and inflammation may play important roles in the pathogenesis of RRDCD. This study may provide novel insights into the pathogenesis of RRDCD and offer potential opportunities for the diagnosis and treatment of RRDCD.
authors.affiliation: Eye Hospital, Nanjing Medical University, Nanjing 210000, China. zhifengwu2013@126.com.|Department of Ophthalmology, Wuxi No. 2 People's Hospital, Nanjing Medical University, Wuxi 214002, China. zhifengwu2013@126.com.
authors.forename: Zhifeng
authors.initials: Z
authors.lastname: Wu
authors.affiliation: Department of Ophthalmology, Wuxi No. 2 People's Hospital, Nanjing Medical University, Wuxi 214002, China. dingnn9009@163.com.
authors.forename: Nannan
authors.initials: N
authors.lastname: Ding
authors.affiliation: Department of Ophthalmology, Wuxi No. 2 People's Hospital, Nanjing Medical University, Wuxi 214002, China. yjmx19860923@126.com.
authors.forename: Mengxi
authors.initials: M
authors.lastname: Yu
authors.affiliation: Key Laboratory of Nuclear Medicine, Ministry of Health, Jiangsu Key Laboratory of Molecular Nuclear Medicine, Jiangsu Institute of Nuclear Medicine, Wuxi 214063, China. wangke@jsinm.org.
authors.forename: Ke
authors.initials: K
authors.lastname: Wang
authors.affiliation: Department of Ophthalmology, Wuxi No. 2 People's Hospital, Nanjing Medical University, Wuxi 214002, China. luoshasha403@163.com.
authors.forename: Shasha
authors.initials: S
authors.lastname: Luo
authors.affiliation: Department of Ophthalmology, Wuxi No. 2 People's Hospital, Nanjing Medical University, Wuxi 214002, China. wendyzwj0805@163.com.
authors.forename: Wenjun
authors.initials: W
authors.lastname: Zou
authors.affiliation: Department of Ophthalmology, Wuxi No. 2 People's Hospital, Nanjing Medical University, Wuxi 214002, China. habbyzy@sina.com.
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: The Fourth School of Clinical Medicine, Nanjing Medical University, Nanjing 210000, China. yanbiao1982@hotmail.com.
authors.forename: Biao
authors.initials: B
authors.lastname: Yan
authors.affiliation: Eye Hospital, Nanjing Medical University, Nanjing 210000, China. jqin710@vip.sina.com.|The Fourth School of Clinical Medicine, Nanjing Medical University, Nanjing 210000, China. jqin710@vip.sina.com.
authors.forename: Qin
authors.initials: Q
authors.lastname: Jiang
mesh_terms: D000328:Adult; D000368:Aged; D015415:Biomarkers / Q000032:analysis*; D019295:Computational Biology; D005260:Female; D020869:Gene Expression Profiling; D063990:Gene Ontology; D006801:Humans; D008297:Male; D008875:Middle Aged; D040901:Proteomics / Q000379:methods*; D012163:Retinal Detachment / Q000378:metabolism*
chemical_list: D015415:Biomarkers
keywords: iTRAQ; proteomics; rhegmatogenous retinal detachment; rhegmatogenous retinal detachment associated with choroidal detachment