title: PLGA/SBA-15 mesoporous silica composite microparticles loaded with paclitaxel for local chemotherapy.
abstract: In this work, high surface area mesoporous silica (SBA-15) was loaded with paclitaxel (taxol, PTX) and was further entrapped into poly(lactic acid-co-glycolic acid) (PLGA) microparticles (MPs). A modified solvent evaporation-emulsion method was used in order to formulate the composite microparticles with sizes of 8-12μm. PTX loaded SBA-15 as well as the PLGA/PTX-SBA-15 composites were characterized in terms of their morphology, crystal structure and thermal properties. Drug content, loading efficiency, particle size and the in-vitro drug release kinetics of the PLGA/PTΧ-SBA-15 microspheres were also investigated. The in vitro release studies were carried out using Simulated Body Fluid (SBF) at 37°C revealing that the prepared formulations present higher dissolution rate than pure PTX and sustained pattern which is ideal for anticancer carriers. Modeling and data analysis of the in vitro drug release was also investigated. It was also shown that all microparticles have low cytotoxicity in HUVE cells. Finally, it was found that drug loaded microparticles are very effective in Human Cervical Adenocarcinoma (HeLa) cells.
authors.affiliation: Laboratory of Polymer Chemistry and Technology, Aristotle University of Thessaloniki, GR-54124 Thessaloniki, Macedonia, Greece.
authors.forename: Stavroula
authors.initials: S
authors.lastname: Nanaki
authors.affiliation: Laboratory of Polymer Chemistry and Technology, Aristotle University of Thessaloniki, GR-54124 Thessaloniki, Macedonia, Greece.
authors.forename: Panoraia I
authors.initials: PI
authors.lastname: Siafaka
authors.affiliation: Laboratory of Polymer Chemistry and Technology, Aristotle University of Thessaloniki, GR-54124 Thessaloniki, Macedonia, Greece.
authors.forename: Dorothea
authors.initials: D
authors.lastname: Zachariadou
authors.affiliation: Laboratory of Polymer Chemistry and Technology, Aristotle University of Thessaloniki, GR-54124 Thessaloniki, Macedonia, Greece.
authors.forename: Maria
authors.initials: M
authors.lastname: Nerantzaki
authors.affiliation: Laboratory of General and Inorganic Chemical Technology, Aristotle University of Thessaloniki, GR-54124 Thessaloniki, Macedonia, Greece.
authors.forename: Dimitrios J
authors.initials: DJ
authors.lastname: Giliopoulos
authors.affiliation: Laboratory of General and Inorganic Chemical Technology, Aristotle University of Thessaloniki, GR-54124 Thessaloniki, Macedonia, Greece.
authors.forename: Konstantinos S
authors.initials: KS
authors.lastname: Triantafyllidis
authors.affiliation: Laboratory of General and Inorganic Chemical Technology, Aristotle University of Thessaloniki, GR-54124 Thessaloniki, Macedonia, Greece.
authors.forename: Margaritis
authors.initials: M
authors.lastname: Kostoglou
authors.affiliation: Laboratory of Biochemistry, Department of Chemistry, Aristotle University of Thessaloniki, GR-54124 Thessaloniki, Macedonia, Greece.
authors.forename: Eleni
authors.initials: E
authors.lastname: Nikolakaki
authors.affiliation: Laboratory of Polymer Chemistry and Technology, Aristotle University of Thessaloniki, GR-54124 Thessaloniki, Macedonia, Greece. Electronic address: dbic@chem.auth.gr.
authors.forename: Dimitrios N
authors.initials: DN
authors.lastname: Bikiaris
mesh_terms: D000970:Antineoplastic Agents / Q000737:chemistry*; D002460:Cell Line; D045744:Cell Line, Tumor; D002626:Chemistry, Pharmaceutical / Q000379:methods; D004337:Drug Carriers / Q000737:chemistry; D004655:Emulsions / Q000737:chemistry; D006367:HeLa Cells; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D019344:Lactic Acid / Q000737:chemistry*; D008863:Microspheres; D017239:Paclitaxel / Q000737:chemistry*; D010316:Particle Size; D011100:Polyglycolic Acid / Q000737:chemistry*; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D012822:Silicon Dioxide / Q000737:chemistry*; D012997:Solvents / Q000737:chemistry
chemical_list: D000970:Antineoplastic Agents; D004337:Drug Carriers; D004655:Emulsions; C509969:SBA-15; D012997:Solvents; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; D012822:Silicon Dioxide; D017239:Paclitaxel
keywords: Composite microparticles; Drug encapsulation; High surface area mesoporous silica; PLGA; Paclitaxel; SBA-15; Topical chemotherapy