title: Characterization of the Cag pathogenicity island in Helicobacter pylori from naturally infected rhesus macaques.
abstract: Helicobacter pylori commonly infects the epithelial layer of the human stomach and in some individuals causes peptic ulcers, gastric adenocarcinoma or gastric lymphoma. Helicobacter pylori is a genetically diverse species, and the most important bacterial virulence factor that increases the risk of developing disease, versus asymptomatic colonization, is the cytotoxin associated gene pathogenicity island (cagPAI). Socially housed rhesus macaques are often naturally infected with H. pylori similar to that which colonizes humans, but little is known about the cagPAI. Here we show that H. pylori strains isolated from naturally infected rhesus macaques have a cagPAI very similar to that found in human clinical isolates, and like human isolates, it encodes a functional type IV secretion system. These results provide further support for the relevance of rhesus macaques as a valid experimental model for H. pylori infection in humans.
authors.affiliation: Departments of Medicine and of Microbiology & Immunology, Center for Comparative Medicine, California National Primate Research Center, University of California Davis, Davis, CA 95616, USA.
authors.forename: Emma C
authors.initials: EC
authors.lastname: Skoog
authors.affiliation: Departments of Medicine and of Microbiology & Immunology, Center for Comparative Medicine, California National Primate Research Center, University of California Davis, Davis, CA 95616, USA.
authors.forename: Samuel L
authors.initials: SL
authors.lastname: Deck
authors.affiliation: Departments of Medicine and of Microbiology & Immunology, Center for Comparative Medicine, California National Primate Research Center, University of California Davis, Davis, CA 95616, USA.
authors.forename: Hasan D
authors.initials: HD
authors.lastname: Entwistle
authors.affiliation: Departments of Medicine and of Microbiology & Immunology, Center for Comparative Medicine, California National Primate Research Center, University of California Davis, Davis, CA 95616, USA.
authors.forename: Lori M
authors.initials: LM
authors.lastname: Hansen
authors.affiliation: Departments of Medicine and of Microbiology & Immunology, Center for Comparative Medicine, California National Primate Research Center, University of California Davis, Davis, CA 95616, USA jvsolnick@ucdavis.edu.
authors.forename: Jay V
authors.initials: JV
authors.lastname: Solnick
mesh_terms: D000818:Animals; D005798:Genes, Bacterial; D044404:Genomic Islands; D016481:Helicobacter Infections / Q000382:microbiology / Q000662:veterinary*; D016480:Helicobacter pylori / Q000235:genetics* / Q000302:isolation & purification; D008253:Macaca mulatta; D018419:Primate Diseases / Q000382:microbiology*; D017385:Sequence Homology; D000069319:Type IV Secretion Systems / Q000235:genetics
chemical_list: D000069319:Type IV Secretion Systems
keywords: CagA; Helicobacter pylori; animal model; cag pathogenicity island; rhesus macaques; type 4 secretion system