title: Effects of 15 Hz square wave magnetic fields on the voltage-gated sodium and potassium channels in prefrontal cortex pyramidal neurons.
abstract: Although magnetic fields have significant effects on neurons, little is known about the mechanisms behind their effects. The present study aimed to measure the effects of magnetic fields on ion channels in cortical pyramidal neurons.
  
  
  
  Cortical pyramidal neurons of Kunming mice were isolated and then subjected to 15 Hz, 1 mT square wave (duty ratio 50%) magnetic fields stimulation. Sodium currents (INa), transient potassium currents (IA) and delayed rectifier potassium currents (IK) were recorded by whole-cell patch clamp method.
  
  
  
  We found that magnetic field exposure depressed channel current densities, and altered the activation kinetics of sodium and potassium channels. The inactivation properties of INaand IAwere also altered.
  
  
  
  Magnetic field exposure alters ion channel function in neurons. It is likely that the structures of sodium and potassium channels were influenced by the applied field. Sialic acid, which is an important component of the channels, could be the molecule responsible for the reported results.
authors.affiliation: a School of Electronics and Information Engineering , Tianjin Polytechnic University , Tianjin , China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Zheng
authors.affiliation: a School of Electronics and Information Engineering , Tianjin Polytechnic University , Tianjin , China.
authors.forename: Jun-Rong
authors.initials: JR
authors.lastname: Dou
authors.affiliation: a School of Electronics and Information Engineering , Tianjin Polytechnic University , Tianjin , China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Gao
authors.affiliation: a School of Electronics and Information Engineering , Tianjin Polytechnic University , Tianjin , China.
authors.forename: Lei
authors.initials: L
authors.lastname: Dong
authors.affiliation: b Tianjin Key Laboratory of Biomedical Detecting Techniques & Instruments , Tianjin University , Tianjin , China.
authors.forename: Gang
authors.initials: G
authors.lastname: Li
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D004307:Dose-Response Relationship, Radiation; D015640:Ion Channel Gating / Q000502:physiology / Q000528:radiation effects*; D051657:KCNQ1 Potassium Channel / Q000502:physiology / Q000528:radiation effects*; D060526:Magnetic Fields; D008297:Male; D051379:Mice; D011188:Potassium / Q000378:metabolism; D017397:Prefrontal Cortex / Q000502:physiology / Q000528:radiation effects; D017966:Pyramidal Cells / Q000502:physiology* / Q000528:radiation effects*; D011829:Radiation Dosage; D012964:Sodium / Q000378:metabolism; D061566:Voltage-Gated Sodium Channels / Q000502:physiology / Q000528:radiation effects*
chemical_list: D051657:KCNQ1 Potassium Channel; D061566:Voltage-Gated Sodium Channels; D012964:Sodium; D011188:Potassium
keywords: 15 Hz square wave magnetic fields; delayed rectifier potassium currents; sodium currents; transient potassium currents; voltage-gated channel