title: Metabolic profile of human coelomic fluid.
abstract: OBJECTIVE
  Till now there is very limited knowledge on the molecular content of coelomic fluid and cells. This study presents the first attempt to elucidate the metabolic profile of such samples.
  
  
  METHODS
  Samples were collected via coelocentesis from 41 women during the first trimester of gestation. Metabolic content was assessed using four different analytical platforms. For targeted analysis a hydrophilic interaction chromatography ultra high performance LC-MS/MS method was applied. Holistic analysis performed by GC-MS, NMR spectroscopy and ion cyclotron ultra-high resolution MS (FT-ICR-MS) instrumentation.
  
  
  CONCLUSIONS
  Our observations suggest coelomic fluid and cells as promising biosamples, rich in metabolites with potential use in mammalian system biology studies.
authors.affiliation: Department of Chemistry, Aristotle University, Thessaloniki, Greece.
authors.forename: C
authors.initials: C
authors.lastname: Virgiliou
authors.affiliation: Department of Chemistry, Aristotle University, Thessaloniki, Greece.
authors.forename: L
authors.initials: L
authors.lastname: Valianou
authors.affiliation: Research Unit Analytical BioGeoChemistry Helmholtz Zentrum Munchen, German, Research Center for Health and Environment Neuherberg, Germany.|Chair of Analytical Foodchemistry, Wissenschaftszentrum Weihenstephan für Ernährung, Landnutzunng und Umwelt, Technische Universität München.
authors.forename: M
authors.initials: M
authors.lastname: Witting
authors.affiliation: Research Unit Analytical BioGeoChemistry Helmholtz Zentrum Munchen, German, Research Center for Health and Environment Neuherberg, Germany.
authors.forename: F
authors.initials: F
authors.lastname: Moritz
authors.affiliation: Institute of Biology, Medicinal Chemistry & Biotechnology, National Hellenic Research Foundation, Athens, Greece.
authors.forename: C
authors.initials: C
authors.lastname: Fotakis
authors.affiliation: Institute of Biology, Medicinal Chemistry & Biotechnology, National Hellenic Research Foundation, Athens, Greece.
authors.forename: P
authors.initials: P
authors.lastname: Zoumpoulakis
authors.affiliation: Department of Chemistry, Aristotle University, Thessaloniki, Greece.
authors.forename: A C
authors.initials: AC
authors.lastname: Chatziioannou
authors.affiliation: Department of Obstetrics & Gynecology, Ioannina University Hospital, Ioannina, Greece.
authors.forename: L
authors.initials: L
authors.lastname: Lazaros
authors.affiliation: Department of Obstetrics & Gynecology, Ioannina University Hospital, Ioannina, Greece.
authors.forename: G
authors.initials: G
authors.lastname: Makrydimas
authors.affiliation: Unit for Human Reproduction, 1st Department of Obstetrics & Gynecology, Medical School, Aristotle University of Thessaloniki, Thessaloniki, Greece.
authors.forename: K
authors.initials: K
authors.lastname: Chatzimeletiou
authors.affiliation: Laboratory of Forensic Medicine & Toxicology, Medical School, Aristotle University of Thessaloniki, Thessaloniki, Greece.
authors.forename: N
authors.initials: N
authors.lastname: Raikos
authors.affiliation: Department of Chemistry, Aristotle University, Thessaloniki, Greece.
authors.forename: G A
authors.initials: GA
authors.lastname: Theodoridis
mesh_terms: D001826:Body Fluids / Q000378:metabolism*; D002853:Chromatography, Liquid; D004622:Embryo, Mammalian / Q000378:metabolism*; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry; D005865:Gestational Age; D058746:Gestational Sac / Q000378:metabolism; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D055442:Metabolome; D055432:Metabolomics; D053719:Tandem Mass Spectrometry
keywords: coelomic fluid; coelomic fluid cells; metabolomics