title: Biogenic synthesis of Zinc oxide nanostructures from Nigella sativa seed: Prospective role as food packaging material inhibiting broad-spectrum quorum sensing and biofilm.
abstract: Bacterial spoilage of food products is regulated by density dependent communication system called quorum sensing (QS). QS control biofilm formation in numerous food pathogens and Biofilms formed on food surfaces act as carriers of bacterial contamination leading to spoilage of food and health hazards. Agents inhibiting or interfering with bacterial QS and biofilm are gaining importance as a novel class of next-generation food preservatives/packaging material. In the present study, Zinc nanostructures were synthesised using Nigella sativa seed extract (NS-ZnNPs). Synthesized nanostructures were characterized hexagonal wurtzite structure of size ~24 nm by UV-visible, XRD, FTIR and TEM. NS-ZnNPs demonstrated broad-spectrum QS inhibition in C. violaceum and P. aeruginosa biosensor strains. Synthesized nanostructures inhibited QS regulated functions of C. violaceum CVO26 (violacein) and elastase, protease, pyocyanin and alginate production in PAO1 significantly. NS-ZnNPs at sub-inhibitory concentrations inhibited the biofilm formation of four-food pathogens viz. C. violaceum 12472, PAO1, L. monocytogenes, E. coli. Moreover, NS-ZnNPs was found effective in inhibiting pre-formed mature biofilms of the four pathogens. Therefore, the broad-spectrum inhibition of QS and biofilm by biogenic Zinc oxide nanoparticles and it is envisaged that these nontoxic bioactive nanostructures can be used as food packaging material and/or as food preservative.
authors.affiliation: Department of Food Science and Nutrition, College of Food and Agriculture, King Saud University, Riyadh-11451, Kingdom of Saudi Arabia.
authors.forename: Nasser A
authors.initials: NA
authors.lastname: Al-Shabib
authors.affiliation: Department of Food Science and Nutrition, College of Food and Agriculture, King Saud University, Riyadh-11451, Kingdom of Saudi Arabia.
authors.forename: Fohad Mabood
authors.initials: FM
authors.lastname: Husain
authors.affiliation: College of Science &General Studies, Alfaisal University, Riyadh 11533, Kingdom of Saudi Arabia.
authors.forename: Faheem
authors.initials: F
authors.lastname: Ahmed
authors.affiliation: Department of Chemistry, College of Science, King Saud University, Riyadh-11451, Kingdom of Saudi Arabia.
authors.forename: Rais Ahmad
authors.initials: RA
authors.lastname: Khan
authors.affiliation: Department of Agricultural Microbiology, Aligarh Muslim University, Aligarh-202002, India.
authors.forename: Iqbal
authors.initials: I
authors.lastname: Ahmad
authors.affiliation: College of Science &General Studies, Alfaisal University, Riyadh 11533, Kingdom of Saudi Arabia.
authors.forename: Edreese
authors.initials: E
authors.lastname: Alsharaeh
authors.affiliation: Protein Research Chair, Department of Biochemistry, College of Science, King Saud University, Riyadh-11451, Kingdom of Saudi Arabia.
authors.forename: Mohd Shahnawaz
authors.initials: MS
authors.lastname: Khan
authors.affiliation: Department of Pharmacognosy, College of Pharmacy, King Saud University, Riyadh-11451, Kingdom of Saudi Arabia.
authors.forename: Afzal
authors.initials: A
authors.lastname: Hussain
authors.affiliation: Department of Pharmacognosy, College of Pharmacy, King Saud University, Riyadh-11451, Kingdom of Saudi Arabia.
authors.forename: Md Tabish
authors.initials: MT
authors.lastname: Rehman
authors.affiliation: Department of Botany, Aligarh Muslim University, Aligarh-202002, India.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Yusuf
authors.affiliation: Department of Zoology, College of Sciences, King Saud University, Riyadh, Saudi Arabia.
authors.forename: Iftekhar
authors.initials: I
authors.lastname: Hassan
authors.affiliation: Department of Food Science and Nutrition, College of Food and Agriculture, King Saud University, Riyadh-11451, Kingdom of Saudi Arabia.
authors.forename: Javed Masood
authors.initials: JM
authors.lastname: Khan
authors.affiliation: King Fahd Medical Research Center, King Abdulaziz University, Jeddah, Saudi Arabia.
authors.forename: Ghulam Md
authors.initials: GM
authors.lastname: Ashraf
authors.affiliation: Department of Chemistry, College of Science, King Saud University, Riyadh-11451, Kingdom of Saudi Arabia.
authors.forename: Ali
authors.initials: A
authors.lastname: Alsalme
authors.affiliation: Protein Research Chair, Department of Biochemistry, College of Science, King Saud University, Riyadh-11451, Kingdom of Saudi Arabia.
authors.forename: Mohamed F
authors.initials: MF
authors.lastname: Al-Ajmi
authors.affiliation: Institute of Pharmacy and Translational Medicine, Sechenov First Moscow State Medical University, 2-4 Bolshaya Pirogovskaya St., 119991 Moscow, Russia.
authors.forename: Vadim V
authors.initials: VV
authors.lastname: Tarasov
authors.affiliation: GALLY International Biomedical Research Consulting LLC., 7733 Louis Pasteur Drive, #330, San Antonio, TX, 78229, USA.|School of Health Science and Healthcare Administration, University of Atlanta, E. Johns Crossing, #175, Johns Creek, GA, 30097, USA.|Institute of Physiologically Active Compounds Russian Academy of Sciences, Chernogolovka, 142432, Russia.
authors.forename: Gjumrakch
authors.initials: G
authors.lastname: Aliev
mesh_terms: D018441:Biofilms / Q000187:drug effects; D002861:Chromobacterium / Q000187:drug effects / Q000378:metabolism / Q000502:physiology; D018857:Food Packaging; D005520:Food Preservatives / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D046529:Microscopy, Electron, Transmission; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure; D031881:Nigella sativa / Q000378:metabolism*; D011446:Prospective Studies; D011550:Pseudomonas aeruginosa / Q000187:drug effects / Q000378:metabolism / Q000502:physiology; D053038:Quorum Sensing / Q000187:drug effects; D012639:Seeds / Q000378:metabolism*; D017550:Spectroscopy, Fourier Transform Infrared; D014961:X-Ray Diffraction; D015034:Zinc Oxide / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology
chemical_list: D005520:Food Preservatives; D015034:Zinc Oxide