title: Analyzing ERK Signal Dynamics During Zebrafish Somitogenesis.
abstract: During vertebrate development, Erk is activated and regulates multiple cellular processes such as cell growth, differentiation, migration, and adhesion in a spatiotemporal manner. Whole-mount immunohistochemistry using antibodies against diphosphorylated Erk (p-Erk; active form of Erk) is a very useful method for understanding the spatial and temporal patterns of Erk activity during embryonic development. However, the fixation step of this method stops embryo development at a certain time point, making it very difficult to observe and interpret Erk activity dynamics. In this chapter, we describe a strategy that combines immunohistochemistry and quantitative analyses of multiple fixed embryos to reconstruct Erk activity dynamics during zebrafish somitogenesis.
authors.affiliation: Gene Regulation Research, Nara Institute of Science and Technology, 8916-5, Takayama, Nara, 630-0101, Japan. matsui@bs.naist.jp.
authors.forename: Takaaki
authors.initials: T
authors.lastname: Matsui
authors.affiliation: Gene Regulation Research, Nara Institute of Science and Technology, 8916-5, Takayama, Nara, 630-0101, Japan.
authors.forename: Yasumasa
authors.initials: Y
authors.lastname: Bessho
mesh_terms: D000818:Animals; D004625:Embryo, Nonmammalian; D047108:Embryonic Development; D005346:Fibroblast Growth Factors / Q000378:metabolism; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D020935:MAP Kinase Signaling System; D018613:Microscopy, Confocal; D019170:Somites / Q000196:embryology*; D015027:Zebrafish / Q000196:embryology* / Q000502:physiology*
chemical_list: D005346:Fibroblast Growth Factors
keywords: FGF; Immunohistochemistry; MAPK; Quantitative analysis; Somite; Zebrafish