title: Comparative proteomic analysis unravels a role for EsrB in the regulation of reactive oxygen species stress responses in Edwardsiella piscicida.
abstract: As a leading pathogen, Edwardsiella piscicida can cause hemorrhagic septicemia in fish and gastro-intestinal infections in humans. The two-component regulatory system EsrA-EsrB plays essential roles in pathogenesis through the type III and type VI secretion systems, and hemolysin production in E. piscicida It is unclear whether other virulence- or stress response-associated genes are regulated by EsrA-EsrB. In this study, the proteomes of wild-type E. piscicida EIB202 and esrB mutant strains were compared to reveal EsrB regulon components after growth in Luria-Bertani broth (LB). Overall, the expression levels of nine genes exhibited significant changes, and five of them required the presence of EsrB, while others exhibited higher levels in the esrB mutant. The diverse functions of these proteins were identified, including amino acid metabolism, oxidative stress defense and energy production. Interestingly, superoxidase dismutase and thiol peroxidase were the most significantly down-regulated by EsrB. Furthermore, other reported reactive oxygen species (ROS) resistance-related genes were also down-regulated by EsrB as revealed by quantitative real-time. Compared with the wild-type and the complement strain esrB+, ΔesrB displayed a significantly enhanced ROS resistance. These results demonstrated that EsrB plays important roles in the ROS resistance pathway in E. piscicida grown in LB conditions.
authors.affiliation: State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China.
authors.forename: Kaiyu
authors.initials: K
authors.lastname: Yin
authors.affiliation: State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China.|Shanghai Engineering Research Center of Maricultured Animal Vaccines, Shanghai, China.|Shanghai Collaborative Innovation Center for Biomanufacturing, Shanghai, China.
authors.forename: Qiyao
authors.initials: Q
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China jfxiao@ecust.edu.cn.|Shanghai Engineering Research Center of Maricultured Animal Vaccines, Shanghai, China.|Shanghai Collaborative Innovation Center for Biomanufacturing, Shanghai, China.
authors.forename: Jingfan
authors.initials: J
authors.lastname: Xiao
authors.affiliation: State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China.|Shanghai Engineering Research Center of Maricultured Animal Vaccines, Shanghai, China.|Shanghai Collaborative Innovation Center for Biomanufacturing, Shanghai, China.
authors.forename: Yuanxing
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics*; D020594:Edwardsiella / Q000235:genetics* / Q000502:physiology*; D004756:Enterobacteriaceae Infections / Q000382:microbiology / Q000662:veterinary; D005393:Fish Diseases / Q000382:microbiology; D015964:Gene Expression Regulation, Bacterial; D006460:Hemolysin Proteins / Q000235:genetics; D006801:Humans; D009154:Mutation; D040901:Proteomics; D017382:Reactive Oxygen Species / Q000378:metabolism*; D013312:Stress, Physiological / Q000235:genetics*; D013482:Superoxide Dismutase / Q000235:genetics; D037521:Virulence Factors / Q000235:genetics
chemical_list: D001426:Bacterial Proteins; D006460:Hemolysin Proteins; D017382:Reactive Oxygen Species; D037521:Virulence Factors; D013482:Superoxide Dismutase
keywords: Edwardsiella piscicida; EsrB, comparative proteomics; ROS