title: Quantification of biologically active O-prenylated and unprenylated phenylpropanoids in dill (Anethum graveolens), anise (Pimpinella anisum), and wild celery (Angelica archangelica).
abstract: An analytical strategy based on different extraction methodologies and HPLC with spectrophotometric (UV-vis) detection has been developed to investigate the presence of and to quantitate biologically active selected unprenylated and O-prenylated phenylpropanoids, namely umbelliferone, 4'-geranyloxyferulic acid, 7-isopentenyloxycoumarin, auraptene, and umbelliprenin in dill (Anethum graveolens L.), anise (Pimpinella anisum L.), and wild celery (Angelica archangelica L.). Absolute ethanol or 7:3 water/ethanol mixtures were seen to be the most powerful extraction solvents to perform "classic" maceration or ultrasound-assisted one in terms of yields in secondary metabolites. For anethum and anise, umbelliprenine was found to be the most abundant prenyloxy secondary metabolite, while in wild celery 4'-geranyloxyferulic acid recorded the highest concentration. Our experimental approach demonstrated to be efficient for the simultaneous identification and quantitation of the above mentioned prenyloxyphenylpropanoids in the title plant species, that is reported herein for the first time in the literature.
authors.affiliation: Department of Pharmacy, University "G. d'Annunzio" - Chieti-Pescara, Via dei Vestini 31, 66100 Chieti, Italy.
authors.forename: Vito Alessandro
authors.initials: VA
authors.lastname: Taddeo
authors.affiliation: Department of Pharmacy, University "G. d'Annunzio" - Chieti-Pescara, Via dei Vestini 31, 66100 Chieti, Italy. Electronic address: s.genovese@unich.it.
authors.forename: Salvatore
authors.initials: S
authors.lastname: Genovese
authors.affiliation: Affichem S.A., 9, Rue de Saint Joseph, 31400 Toulouse, France.
authors.forename: Philippe de
authors.initials: P
authors.lastname: Medina
authors.affiliation: Department of Pharmacy, University "G. d'Annunzio" - Chieti-Pescara, Via dei Vestini 31, 66100 Chieti, Italy.
authors.forename: Roberta
authors.initials: R
authors.lastname: Palmisano
authors.affiliation: Department of Pharmacy, University "G. d'Annunzio" - Chieti-Pescara, Via dei Vestini 31, 66100 Chieti, Italy.
authors.forename: Francesco
authors.initials: F
authors.lastname: Epifano
authors.affiliation: Department of Pharmacy, University "G. d'Annunzio" - Chieti-Pescara, Via dei Vestini 31, 66100 Chieti, Italy.
authors.forename: Serena
authors.initials: S
authors.lastname: Fiorito
mesh_terms: D028524:Anethum graveolens; D029970:Angelica archangelica; D064209:Phytochemicals / Q000032:analysis* / Q000737:chemistry; D028042:Pimpinella; D010936:Plant Extracts / Q000032:analysis* / Q000737:chemistry; D018517:Plant Roots; D054876:Prenylation; D012639:Seeds
chemical_list: D064209:Phytochemicals; D010936:Plant Extracts
keywords: Anethum graveolens; Angelica archangelica; HPLC–UV–vis; Phytochemicals; Pimpinella anisum