title: Anaplasma platys in dogs from Uruguay.
abstract: Anaplasmataceae family members include vector-borne bacteria of veterinary importance that may also affect humans. Ehrlichia canis and Anaplasma platys are the main members of this family detected in dogs worldwide. In Uruguay there are not many published studies on tick-borne pathogens affecting dogs, the only haemoparasite molecularly confirmed in dogs, is the piroplasm Rangelia vitalii. The aim of the present work was to detect the presence of A. platys and E. canis in dogs and dogs-associated ticks of two localities in Northwestern Uruguay. Blood samples from dogs with and without clinical signs associated with vector-borne diseases, and Rhipicephalus sanguineus obtained from these dogs were analyzed by PCR for Anaplasmataceae. Positive dogs were further analyzed by PCR for Ehrlichia spp. and A. platys. All the ticks were found negative. No dog was detected infected with E. canis, while eight dogs (4.2%) were found to be infected with A. platys. Phylogenetic analysis of groESL operon sequence for A. platys revealed no differences with sequences described for A. platys in neighbor countries and from other regions of the world. This is the first report of the presence of A. platys in Uruguay.
authors.affiliation: Laboratorio de Vectores y Enfermedades Transmitidas, Facultad de Veterinaria, CENUR Litoral Norte - Salto, Universidad de la República, Rivera 1350, CP 50000, Salto, Uruguay. Electronic address: luisandrescarvalho@gmail.com.
authors.forename: Luis
authors.initials: L
authors.lastname: Carvalho
authors.affiliation: Laboratorio de Vectores y Enfermedades Transmitidas, Facultad de Veterinaria, CENUR Litoral Norte - Salto, Universidad de la República, Rivera 1350, CP 50000, Salto, Uruguay. Electronic address: m.teresa.armua@gmail.com.
authors.forename: Maria Teresa
authors.initials: MT
authors.lastname: Armua-Fernandez
authors.affiliation: Laboratorio de Vectores y Enfermedades Transmitidas, Facultad de Veterinaria, CENUR Litoral Norte - Salto, Universidad de la República, Rivera 1350, CP 50000, Salto, Uruguay. Electronic address: nicolas317@vera.com.uy.
authors.forename: Nicolás
authors.initials: N
authors.lastname: Sosa
authors.affiliation: Facultad de Veterinaria, CENUR Litoral Norte - Salto, Universidad de la República, Rivera 1350, CP 50000, Salto, Uruguay. Electronic address: mlfelix78@hotmail.com.
authors.forename: María Laura
authors.initials: ML
authors.lastname: Félix
authors.affiliation: Laboratorio de Vectores y Enfermedades Transmitidas, Facultad de Veterinaria, CENUR Litoral Norte - Salto, Universidad de la República, Rivera 1350, CP 50000, Salto, Uruguay. Electronic address: dpvuru@hotmail.com.
authors.forename: José Manuel
authors.initials: JM
authors.lastname: Venzal
mesh_terms: D000709:Anaplasma / Q000145:classification / Q000302:isolation & purification*; D000712:Anaplasmosis / Q000453:epidemiology / Q000382:microbiology*; D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D018833:Chaperonins / Q000235:genetics / Q000378:metabolism; D004283:Dog Diseases / Q000453:epidemiology / Q000382:microbiology*; D004285:Dogs; D005260:Female; D015964:Gene Expression Regulation, Bacterial; D008297:Male; D009758:Nymph / Q000382:microbiology; D010802:Phylogeny; D014583:Uruguay / Q000453:epidemiology
chemical_list: D001426:Bacterial Proteins; C097970:GroESL protein, Bacteria; D018833:Chaperonins
keywords: Anaplasmataceae; Dogs; Ehrlichia canis; Rhipicephalus sanguineus; Uruguay