title: Lipoprotein profiling methodology based on determination of apolipoprotein concentration.
abstract: OBJECTIVE
  Abnormal lipid metabolism results in the alteration of lipid compositions in lipoproteins; therefore an accurate and quantitative analytical approach is required for the detailed structural characterization of lipoproteins. However, the specific lipid composition of each lipoprotein particle is poorly understood.
  
  
  METHODS
  Lipid composition of very-low-density lipoprotein and low-density lipoprotein particles derived from myocardial infarction-prone rabbits was determined by normalization of lipidomics data using apoB-100 levels.
  
  
  RESULTS
  The ratio of lipid levels between very-low-density lipoprotein and low-density lipoprotein particles was different according to not only lipid classes, but also phosphatidylethanolamine subclasses by applying our developed methodology to myocardial infarction-prone rabbits.
  
  
  CONCLUSIONS
  Our novel analytical approach represents to be a potentially useful tool to obtain particle-specific lipid components of lipoproteins.
authors.affiliation: Division of Metabolomics, Medical Institute of Bioregulation, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812 8582, Japan.
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Takeda
authors.affiliation: Division of Metabolomics, Medical Institute of Bioregulation, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812 8582, Japan.
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Izumi
authors.affiliation: Department of Bioinformatic Engineering, Graduate School of Information Science & Technology, Osaka University, 1-5 Yamadaoka, Suita, Osaka 565 0871, Japan.
authors.forename: Atsumi
authors.initials: A
authors.lastname: Tomita
authors.affiliation: Institute for Experimental Animals, Kobe University Graduate School of Medicine, 7-5-1 Kusunoki-cho, Chuo-ku, Kobe 650 0017, Japan.
authors.forename: Tomonari
authors.initials: T
authors.lastname: Koike
authors.affiliation: Institute for Experimental Animals, Kobe University Graduate School of Medicine, 7-5-1 Kusunoki-cho, Chuo-ku, Kobe 650 0017, Japan.|Division of Comparative Pathophysiology, Department of Physiology & Cell Biology, Kobe University Graduate School of Medicine, 7-5-1 Kusunoki-cho, Chuo-ku, Kobe 650 0017, Japan.
authors.forename: Masashi
authors.initials: M
authors.lastname: Shiomi
authors.affiliation: Department of Biotechnology, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565 0871, Japan.
authors.forename: Eiichiro
authors.initials: E
authors.lastname: Fukusaki
authors.affiliation: Department of Bioinformatic Engineering, Graduate School of Information Science & Technology, Osaka University, 1-5 Yamadaoka, Suita, Osaka 565 0871, Japan.
authors.forename: Fumio
authors.initials: F
authors.lastname: Matsuda
authors.affiliation: Division of Metabolomics, Medical Institute of Bioregulation, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812 8582, Japan.
authors.forename: Takeshi
authors.initials: T
authors.lastname: Bamba
mesh_terms: D000818:Animals; D053299:Apolipoprotein B-100 / Q000032:analysis / Q000097:blood; D001053:Apolipoproteins / Q000032:analysis* / Q000097:blood; D002853:Chromatography, Liquid / Q000379:methods; D005260:Female; D008074:Lipoproteins / Q000032:analysis* / Q000097:blood; D008077:Lipoproteins, LDL / Q000032:analysis / Q000097:blood; D008079:Lipoproteins, VLDL / Q000032:analysis / Q000097:blood; D008297:Male; D009203:Myocardial Infarction / Q000097:blood / Q000473:pathology; D011817:Rabbits; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D053299:Apolipoprotein B-100; D001053:Apolipoproteins; D008074:Lipoproteins; D008077:Lipoproteins, LDL; D008079:Lipoproteins, VLDL
keywords: apolipoprotein; lipoprotein; targeted proteomics