title: Matrilin-2 regulates proliferation, apoptosis and cell cycle during radiation-induced injury in HPAEpiC cell.
abstract: Radiation pulmonary injury is related to the accumulation of extracellular matrix proteins in the alveolar interstitial space. Matrilin-2 as a component of extracellular filamentous networks, present higher level in the lung tissue from irradiated mice and irradiated pulmonary epithelial cell line, HPAEpiC cells. Knockdown of endogenous matrilin-2 prevents the apoptosis of HPAEpiC cell induced by the irradiation injury. Consistently, over-expression of matrilin-2 reduced the proliferation and induced apoptosis of HPAEpiC cells. Matrilin-2 promotes the expression of p21 via increasing the transcriptional activity of p53, by which induces the G1 phase arresting in HPAEpiC cells. In summary, matrilin-2, increased by irradiation, reduced the proliferation and induces apoptosis of pulmonary epithelial cells via p53/p21 pathway.
authors.affiliation: Department of Pathology, Qinghai People's Provincial Hospital, Xining 810007, Qinghai Province, People's Republic of China.
authors.forename: Junming
authors.initials: J
authors.lastname: Luo
authors.affiliation: Department of Pathology, Qinghai People's Provincial Hospital, Xining 810007, Qinghai Province, People's Republic of China.
authors.forename: Menglan
authors.initials: M
authors.lastname: Zhang
authors.affiliation: Department of Pathology, Qinghai People's Provincial Hospital, Xining 810007, Qinghai Province, People's Republic of China.
authors.forename: Hong
authors.initials: H
authors.lastname: Huang
authors.affiliation: Department of Anesthesiology, Hunan Provincial Tumor Hospital, Central South University, Changsha 410013, Hunan Province, People's Republic of China.
authors.forename: Yichun
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Internal Medicine, Qinghai People's Provincial Hospital, Xining 810007, Qinghai Province, People's Republic of China.
authors.forename: Xin
authors.initials: X
authors.lastname: Yuan
authors.affiliation: Department of Critical Care Medicine, Qinghai People's Provincial Hospital, Xining 810007, Qinghai Province, People's Republic of China.
authors.forename: Siqing
authors.initials: S
authors.lastname: Ma
authors.affiliation: Department of Anesthesiology, Hunan Provincial Tumor Hospital, Central South University, Changsha 410013, Hunan Province, People's Republic of China.
authors.forename: Jingshi
authors.initials: J
authors.lastname: Liu
authors.affiliation: Department of Pathology, Qinghai People's Provincial Hospital, Xining 810007, Qinghai Province, People's Republic of China.
authors.forename: Shiying
authors.initials: S
authors.lastname: Zhou
authors.affiliation: Department of Pathology, Qinghai People's Provincial Hospital, Xining 810007, Qinghai Province, People's Republic of China. Electronic address: zsk0475@163.com.
authors.forename: Shukun
authors.initials: S
authors.lastname: Zhang
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000235:genetics* / Q000528:radiation effects; D015153:Blotting, Western; D049109:Cell Proliferation / Q000235:genetics* / Q000528:radiation effects; D002478:Cells, Cultured; D050759:Cyclin-Dependent Kinase Inhibitor p21 / Q000235:genetics / Q000378:metabolism; D004847:Epithelial Cells / Q000378:metabolism* / Q000528:radiation effects; D059585:G1 Phase Cell Cycle Checkpoints / Q000235:genetics* / Q000528:radiation effects; D015870:Gene Expression / Q000528:radiation effects; D006801:Humans; D008168:Lung / Q000378:metabolism / Q000528:radiation effects; D008297:Male; D064235:Matrilin Proteins / Q000235:genetics* / Q000378:metabolism; D008810:Mice, Inbred C57BL; D011650:Pulmonary Alveoli / Q000166:cytology; D034622:RNA Interference; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015398:Signal Transduction / Q000235:genetics / Q000528:radiation effects; D016159:Tumor Suppressor Protein p53 / Q000235:genetics / Q000378:metabolism
chemical_list: D050759:Cyclin-Dependent Kinase Inhibitor p21; D064235:Matrilin Proteins; D016159:Tumor Suppressor Protein p53
keywords: Apoptosis; Cell cycle; Matrilin-2; Proliferation; Radiation pulmonary injury