title: Enterobacter sp. LU1 as a novel succinic acid producer - co-utilization of glycerol and lactose.
abstract: Succinic acid is an important C4-building chemical platform for many applications. A novel succinic acid-producing bacterial strain was isolated from goat rumen. Phylogenetic analysis based on the 16S rRNA sequence and physiological analysis indicated that the strain belongs to the genus Enterobacter. This is the first report of a wild bacterial strain from the genus Enterobacter that is capable of efficient succinic acid production. Co-fermentation of glycerol and lactose significantly improved glycerol utilization under anaerobic conditions, debottlenecking the utilization pathway of this valuable biodiesel waste product. Succinic acid production reached 35 g l-1when Enterobacter sp. LU1 was cultured in medium containing 50 g l-1of glycerol and 25 g l-1of lactose as carbon sources.
authors.affiliation: Department of Biotechnology, Human Nutrition and Food Commodities, Lublin University of Life Sciences, 8 Skromna, Lublin, 20-704, Poland.
authors.forename: Marcin
authors.initials: M
authors.lastname: Podleśny
authors.affiliation: Department of Biotechnology, Human Nutrition and Food Commodities, Lublin University of Life Sciences, 8 Skromna, Lublin, 20-704, Poland.
authors.forename: Piotr
authors.initials: P
authors.lastname: Jarocki
authors.affiliation: Department of Analysis and Food Quality Assessment, Lublin University of Life Sciences, 8 Skromna, Lublin, 20-704, Poland.
authors.forename: Jakub
authors.initials: J
authors.lastname: Wyrostek
authors.affiliation: Department of Biotechnology, Human Nutrition and Food Commodities, Lublin University of Life Sciences, 8 Skromna, Lublin, 20-704, Poland.
authors.forename: Tomasz
authors.initials: T
authors.lastname: Czernecki
authors.affiliation: Department of Biotechnology, Human Nutrition and Food Commodities, Lublin University of Life Sciences, 8 Skromna, Lublin, 20-704, Poland.
authors.forename: Jagoda
authors.initials: J
authors.lastname: Kucharska
authors.affiliation: Department of Biotechnology, Human Nutrition and Food Commodities, Lublin University of Life Sciences, 8 Skromna, Lublin, 20-704, Poland.
authors.forename: Anna
authors.initials: A
authors.lastname: Nowak
authors.affiliation: Department of Biotechnology, Human Nutrition and Food Commodities, Lublin University of Life Sciences, 8 Skromna, Lublin, 20-704, Poland.
authors.forename: Zdzisław
authors.initials: Z
authors.lastname: Targoński
mesh_terms: D000332:Aerobiosis; D000818:Animals; D015373:Bacterial Typing Techniques; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D004754:Enterobacter / Q000145:classification / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D005285:Fermentation; D005990:Glycerol / Q000378:metabolism*; D006041:Goats; D007785:Lactose / Q000378:metabolism*; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012417:Rumen / Q000382:microbiology; D017422:Sequence Analysis, DNA; D019802:Succinic Acid / Q000378:metabolism*
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S; D019802:Succinic Acid; D007785:Lactose; D005990:Glycerol