title: Development of a High-Resolution Melting Analysis Method for CYP2C19*17 Genotyping in Healthy Volunteers.
abstract: BACKGROUND
  Genetic polymorphisms of drug metabolisms by cytochrome P450 (P450s) could affect drug response, attracting particular interest in the pharmacogenetics. Due to the importance of CYP2C19* 17 allele and its capability of super- fast metabolism and also lack of information about distribution of the alleles in Iranian population, this research aimed to use High Resolution Melting (HRM) method compared to PCR-RFLP for genotyping healthy Iranian population.
  
  
  METHODS
  Blood samples were collected from 100 healthy Iranian volunteers. DNA was extracted by salting out method. Real-time PCR was used for amplification of the CYP2C19 gene and the alleles were identified by HRM. Sequencing was used to confirm the amplified DNA fragments and data were analyzed using SPSS software ver.18.
  
  
  RESULTS
  The frequency of alleles CYP2C19*1/*1, CYP2C19*1/*17 and CYP2C19*17/*17 were estimated as 58.33, 29.1 and 11.1%, respectively. Specificity and sensitivity of HRM method were 90% and 100%, with respect to PCR-RFLP. Also, HRM analysis has been evaluated as a faster and more effective approach.
  
  
  CONCLUSIONS
  Comparison of our results based on HRM analysis with PCR-RFLP showed that our developed method is rapid, accurate, fast and economic to study the CYP2C19*17 allele and it is appropriate for other similar population genetic studies.
authors.affiliation: Department of Genetics, Tehran Medical Sciences Branch, Islamic Azad University, Tehran, Iran.
authors.forename: Zahra
authors.initials: Z
authors.lastname: Ghasemi
authors.affiliation: Department of Genetics, Tehran Medical Sciences Branch, Islamic Azad University, Tehran, Iran.
authors.forename: Mehrdad
authors.initials: M
authors.lastname: Hashemi
authors.affiliation: Department of Biotechnology, Faculty of Pharmacy, Zanjan University of Medical Sciences, Zanjan, Iran.
authors.forename: Mahsa
authors.initials: M
authors.lastname: Ejabati
authors.affiliation: Department of Medical Surgical Nursing, Abhar Faculty of Nursing, Zanjan University of Medical Sciences, Zanjan, Iran.
authors.forename: Seyyed Meisam
authors.initials: SM
authors.lastname: Ebrahimi
authors.affiliation: Department of Biotechnology, Faculty of Pharmacy, Zanjan University of Medical Sciences, Zanjan, Iran.
authors.forename: Hamidreza
authors.initials: H
authors.lastname: Kheiri Manjili
authors.affiliation: Department of Biotechnology, Faculty of Pharmacy, Zanjan University of Medical Sciences, Zanjan, Iran.
authors.forename: Ali
authors.initials: A
authors.lastname: Sharafi
authors.affiliation: Department of Biotechnology, Faculty of Pharmacy, Zanjan University of Medical Sciences, Zanjan, Iran.
authors.forename: Ali
authors.initials: A
authors.lastname: Ramazani
keywords: Cytochrome P-450 CYP2C19; Pharmacogenetics; Real-Time Polymerase Chain Reaction