title: Surface Plasmon Resonance: A Useful Strategy for the Identification of Small Molecule Argonaute 2 Protein Binders.
abstract: Surface plasmon resonance (SPR) is one of the most important techniques for the detection and the characterization of molecular interactions. SPR technology is a label-free approach for monitoring biomolecular interactions in real time. The binding of analytes to molecules immobilized on a thin metal film (ligand) determines a change in the refractive index and, therefore in the angle of extinction of light, is reflected when polarized light hits the film, monitored in real time as a change in the position of the dip in reflected intensity. Since SPR detects mass, the technique is label-free.Here, we describe the use of SPR techniques to study the interaction between Argonaute 2 and small molecular compounds selected by means of high-throughput docking screening.
authors.affiliation: CNR-National Research Council of Italy, Institute of Molecular Biology and Pathology, Department of Biochemical Sciences "A. Rossi Fanelli", Sapienza University of Rome, P.le A. Moro 5, 00185, Rome, Italy.
authors.forename: Elena
authors.initials: E
authors.lastname: Poser
authors.affiliation: CNR-National Research Council of Italy, Institute of Molecular Biology and Pathology, Department of Biochemical Sciences "A. Rossi Fanelli", Sapienza University of Rome, P.le A. Moro 5, 00185, Rome, Italy.
authors.forename: Ilaria
authors.initials: I
authors.lastname: Genovese
authors.affiliation: Department of Anatomical, Histological, Forensic & Orthopaedic Sciences, Section of Histology & Medical Embryology, Sapienza University of Rome, Via A. Scarpa 14-16, 00161, Rome, Italy.
authors.forename: Silvia
authors.initials: S
authors.lastname: Masciarelli
authors.affiliation: Department of Anatomical, Histological, Forensic & Orthopaedic Sciences, Section of Histology & Medical Embryology, Sapienza University of Rome, Via A. Scarpa 14-16, 00161, Rome, Italy.
authors.forename: Teresa
authors.initials: T
authors.lastname: Bellissimo
authors.affiliation: Department of Anatomical, Histological, Forensic & Orthopaedic Sciences, Section of Histology & Medical Embryology, Sapienza University of Rome, Via A. Scarpa 14-16, 00161, Rome, Italy. francesco.fazi@uniroma1.it.
authors.forename: Francesco
authors.initials: F
authors.lastname: Fazi
authors.affiliation: IBPM - CNR Institute of Molecular Biology and Pathology, Italian National Research Council, Department of Biochemical Sciences "A. Rossi Fanelli", Sapienza University of Rome, P.le A. Moro 5, 00185, Rome, Italy. gianni.colotti@uniroma1.it.
authors.forename: Gianni
authors.initials: G
authors.lastname: Colotti
mesh_terms: D060565:Argonaute Proteins / Q000737:chemistry / Q000378:metabolism*; D057166:High-Throughput Screening Assays / Q000379:methods*; D006801:Humans; D008024:Ligands; D035683:MicroRNAs / Q000037:antagonists & inhibitors / Q000235:genetics; D062105:Molecular Docking Simulation / Q000379:methods; D011485:Protein Binding; D054852:Small Molecule Libraries / Q000737:chemistry* / Q000627:therapeutic use; D020349:Surface Plasmon Resonance / Q000379:methods*
chemical_list: C466422:AGO2 protein, human; D060565:Argonaute Proteins; D008024:Ligands; D035683:MicroRNAs; D054852:Small Molecule Libraries
keywords: Analyte; Ligand; Protein-inhibitor interaction; Small molecules; Surface plasmon resonance