title: Generation of MERRF patient-derived induced pluripotent stem cell line iMERRF-C7.
abstract: Human iPSC line iMERRF-C7 was generated from PBMCs of a patient with mitochondrial disorder MERRF. Using Sendai virus, the reprogramming factors Oct3/4, Sox2, Klf4, and cMyc were delivered non-integratively. The resulting iPSCs expressed pluripotency markers, could differentiate into the three germ layers in vivo, had normal genomic structure, and retained the disease-causing m.8344 mutation with similar heteroplasmic level.
authors.affiliation: Department of Prenatal Diagnosis, Obstretrics and Gynecology Hospital Affiliated to Nanjing Medical University, Nanjing, 210004, Jiangsu, China.
authors.forename: Dong
authors.initials: D
authors.lastname: Liang
authors.affiliation: Department of Prenatal Diagnosis, Obstretrics and Gynecology Hospital Affiliated to Nanjing Medical University, Nanjing, 210004, Jiangsu, China.
authors.forename: Huanran
authors.initials: H
authors.lastname: Hu
authors.affiliation: Department of Prenatal Diagnosis, Obstretrics and Gynecology Hospital Affiliated to Nanjing Medical University, Nanjing, 210004, Jiangsu, China.
authors.forename: Tianhui
authors.initials: T
authors.lastname: Xu
authors.affiliation: Department of Prenatal Diagnosis, Obstretrics and Gynecology Hospital Affiliated to Nanjing Medical University, Nanjing, 210004, Jiangsu, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Prenatal Diagnosis, Obstretrics and Gynecology Hospital Affiliated to Nanjing Medical University, Nanjing, 210004, Jiangsu, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Hu
authors.affiliation: Department of Prenatal Diagnosis, Obstretrics and Gynecology Hospital Affiliated to Nanjing Medical University, Nanjing, 210004, Jiangsu, China. Electronic address: njxzf@126.com.
authors.forename: Zhengfeng
authors.initials: Z
authors.lastname: Xu
mesh_terms: D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D065150:Cellular Reprogramming; D005260:Female; D056426:Genetic Loci; D005822:Genetic Vectors / Q000235:genetics / Q000378:metabolism; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000166:cytology* / Q000378:metabolism / Q000637:transplantation; D059785:Karyotype; D000090062:Kruppel-Like Factor 4; D007963:Leukocytes, Mononuclear / Q000166:cytology; D017243:MERRF Syndrome / Q000235:genetics / Q000473:pathology; D051379:Mice; D016688:Mice, Inbred NOD; D016513:Mice, SCID; D008856:Microscopy, Fluorescence; D020641:Polymorphism, Single Nucleotide; D029082:Sendai virus / Q000235:genetics; D017422:Sequence Analysis, DNA; D013724:Teratoma / Q000473:pathology; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism
chemical_list: C000715467:KLF4 protein, human; C000715468:Klf4 protein, mouse; D000090062:Kruppel-Like Factor 4; D014157:Transcription Factors