title: Restorative effects of hydroxysafflor yellow A on hepatic function in an experimental regression model of hepatic fibrosis induced by carbon tetrachloride.
abstract: Hepatic fibrosis is a reversible pathological process, in which fibrotic tissue is excessively deposited in the liver during the repair process that follows hepatic injury. Early prevention or treatment of hepatic fibrosis has great significance on the treatment of chronic hepatic diseases. Hydroxysafflor yellow A (HSYA) is a water-soluble monomer extracted from safflower, which serves numerous pharmacological roles. However, it remains to be elucidated how HSYA regulates hepatic fibrogenesis. The aim of the present study was to reveal the possible mechanisms underlying the effects of HSYA on the prevention and treatment of hepatic fibrosis. A rat model of hepatic fibrosis was established in the present study, and the rats were administered various doses of HSYA. The effects of HSYA on pathological alterations of the liver tissue in rats with hepatic fibrosis were observed using hematoxylin-eosin staining and Masson staining. In order to explore the anti‑hepatic fibrosis effects and underlying mechanisms of HSYA, serum levels, and hepatic function and hepatic fibrosis indices were evaluated. The results demonstrated that HSYA can improve the general condition of rats with hepatic fibrosis and relieve cellular swelling of the liver, fatty degeneration, necrosis, inflammatory cell infiltration and fibroplastic proliferation. Subsequent to administration of HSYA, globulin was increased during hepatic fibrosis caused by tetrachloromethane. However, total cholesterol, triglyceride, alanine aminotransferase, aspartate aminotransferase and levels of hyaluronic acid, laminin, procollagen Ⅲ N‑terminal peptide, collagen type Ⅳ and hydroxyproline were significantly reduced. The results additionally demonstrated that HSYA could enhance superoxide dismutase activity and reduce malondialdehyde levels, inhibiting lipid peroxidation caused by free radicals.
authors.affiliation: Department of Histology and Embryology, Binzhou Medical University, Yantai, Shandong 264003, P.R. China.
authors.forename: Yanuo
authors.initials: Y
authors.lastname: Li
authors.affiliation: Department of Histology and Embryology, Binzhou Medical University, Yantai, Shandong 264003, P.R. China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Shi
authors.affiliation: Department of Osteology, Yantaishan Hospital, Yantai, Shandong 264000, P.R. China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Sun
authors.affiliation: Department of Pathology, Binzhou Medical University, Yantai, Shandong 264003, P.R. China.
authors.forename: Luying
authors.initials: L
authors.lastname: Liu
authors.affiliation: Department of Histology and Embryology, Binzhou Medical University, Yantai, Shandong 264003, P.R. China.
authors.forename: Xianyong
authors.initials: X
authors.lastname: Bai
authors.affiliation: Department of Histology and Embryology, Binzhou Medical University, Yantai, Shandong 264003, P.R. China.
authors.forename: Dong
authors.initials: D
authors.lastname: Wang
authors.affiliation: Department of Histology and Embryology, Binzhou Medical University, Yantai, Shandong 264003, P.R. China.
authors.forename: Hongxing
authors.initials: H
authors.lastname: Li
mesh_terms: D000410:Alanine Transaminase / Q000097:blood / Q000378:metabolism; D000818:Animals; D001219:Aspartate Aminotransferases / Q000097:blood / Q000378:metabolism; D002251:Carbon Tetrachloride; D031187:Carthamus tinctorius / Q000737:chemistry; D002599:Chalcone / Q000031:analogs & derivatives* / Q000737:chemistry / Q000627:therapeutic use; D050356:Lipid Metabolism / Q000187:drug effects; D008099:Liver / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology*; D008106:Liver Cirrhosis, Experimental / Q000139:chemically induced / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology*; D008297:Male; D011809:Quinones / Q000737:chemistry / Q000627:therapeutic use*; D017207:Rats, Sprague-Dawley
chemical_list: D011809:Quinones; C085278:hydroxysafflor yellow A; D002599:Chalcone; D002251:Carbon Tetrachloride; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase