title: Fast Screening of Antibacterial Compounds from Fusaria.
abstract: Bio-guided screening is an important method to identify bioactive compounds from fungi. In this study we applied a fast digital time-lapse microscopic method for assessment of the antibacterial properties of secondary metabolites from the fungal genusFusarium. Here antibacterial effects could be detected for antibiotic Y, aurofusarin, beauvericin, enniatins and fusaric acid after six hours of cultivation. The system was then used in a bio-guided screen of extracts from 14 differentFusariumspecies, which had been fractionated by HPLC. In this screen, fractions containing the red pigments aurofusarin and bikaverin showed effects against strains ofLactobacillusandBifidobacterium. The IC50for aurofusarin againstLactobacillus acidophiluswas 8 µM, and againstBifidobacterium breveit was 64 µM. Aurofusarin only showed an effect on probiotic bacteria, leading to the speculation that only health-promoting bacteria with a positive effect in the gut system are affected.
authors.affiliation: Department of Chemistry and Bioscience, Aalborg University, Frederik Bajers Vej 7H, DK-9220 Aalborg Ø, Denmark. tes@bio.aau.dk.
authors.forename: Teis Esben
authors.initials: TE
authors.lastname: Sondergaard
authors.affiliation: Department of Animal Science, Faculty of Science and Technology, Aarhus University, Blichers Allé 20, DK-8830 Tjele, Denmark. marlene.fredborg@anis.au.dk.
authors.forename: Marlene
authors.initials: M
authors.lastname: Fredborg
authors.affiliation: Department of Chemistry and Bioscience, Aalborg University, Frederik Bajers Vej 7H, DK-9220 Aalborg Ø, Denmark. a-mariac@hotmail.com.
authors.forename: Ann-Maria
authors.initials: AM
authors.lastname: Oppenhagen Christensen
authors.affiliation: Department of Chemistry and Bioscience, Aalborg University, Frederik Bajers Vej 7H, DK-9220 Aalborg Ø, Denmark. baldurskd@hotmail.com.
authors.forename: Sofie K
authors.initials: SK
authors.lastname: Damsgaard
authors.affiliation: Department of Chemistry and Bioscience, Aalborg University, Frederik Bajers Vej 7H, DK-9220 Aalborg Ø, Denmark. nikolinekramer@hotmail.com.
authors.forename: Nikoline F
authors.initials: NF
authors.lastname: Kramer
authors.affiliation: Department of Chemistry and Bioscience, Aalborg University, Frederik Bajers Vej 7H, DK-9220 Aalborg Ø, Denmark. hgiese@bio.aau.dk.
authors.forename: Henriette
authors.initials: H
authors.lastname: Giese
authors.affiliation: Department of Chemistry and Bioscience, Aalborg University, Frederik Bajers Vej 7H, DK-9220 Aalborg Ø, Denmark. jls@bio.aau.dk.|Department of Chemistry and Bioscience, Aalborg University, Niels Bohrs Vej 8, 6700 Esbjerg, Denmark. jls@bio.aau.dk.
authors.forename: Jens Laurids
authors.initials: JL
authors.lastname: Sørensen
mesh_terms: D000900:Anti-Bacterial Agents / Q000096:biosynthesis / Q000494:pharmacology*; D001419:Bacteria / Q000187:drug effects* / Q000254:growth & development; D047630:Depsipeptides / Q000494:pharmacology; D005669:Fusaric Acid / Q000494:pharmacology; D005670:Fusarium / Q000378:metabolism*; D009183:Mycotoxins / Q000494:pharmacology; D009285:Naphthoquinones / Q000494:pharmacology
chemical_list: D000900:Anti-Bacterial Agents; D047630:Depsipeptides; D009183:Mycotoxins; D009285:Naphthoquinones; C100264:enniatins; C048670:antibiotic Y; C004456:beauvericin; C083578:aurofusarin; D005669:Fusaric Acid
keywords: Fusarium; antibacterial; antibiotics; aurofusarin; bio-guided assays; bioactivity; mycotoxins; pigments; polyketides; secondary metabolites