title: Relationships between sulfachloropyridazine sodium, zinc, and sulfonamide resistance genes during the anaerobic digestion of swine manure.
abstract: In this study, swine manure containing sulfachloropyridazine sodium (SCPS) and zinc was subjected to mesophilic (37°C) anaerobic digestion (AD). The absolute abundances (AAs) of antibiotic resistance genes (ARGs) were evaluated, as well as intI1 and intI2, and the degradation of SCPS according to variation in the amount of bio-available zinc (bio-Zn). In digester that only contained SCPS, the concentrations of SCPS were lower than that digesters both contain SCPS and Zn. Compared with the control digester, the addition of SCPS increased the AAs of sul1, sul3, drfA1, and drfA7 by 1.3-13.1 times. However, compared with the digester with SCPS but no added Zn, the AAs of sul3, drfA1, and drfA7 were decreased by 21.4-70.3% in the presence of SCPS and Zn, whereas sul1 and sul2 increased 1.3-10.7 times. There were significant positive correlations (P<0.05) between the concentrations of SCPS with several ARGs and bio-Zn.
authors.affiliation: College of Natural Resources and Environment, Northwest A&F University, Yangling, Shaanxi 712100, China.
authors.forename: Ranran
authors.initials: R
authors.lastname: Zhang
authors.affiliation: College of Natural Resources and Environment, Northwest A&F University, Yangling, Shaanxi 712100, China. Electronic address: gujie205@sina.com.
authors.forename: Jie
authors.initials: J
authors.lastname: Gu
authors.affiliation: College of Natural Resources and Environment, Northwest A&F University, Yangling, Shaanxi 712100, China.
authors.forename: Xiaojuan
authors.initials: X
authors.lastname: Wang
authors.affiliation: College of Natural Resources and Environment, Northwest A&F University, Yangling, Shaanxi 712100, China.
authors.forename: Xun
authors.initials: X
authors.lastname: Qian
authors.affiliation: College of Natural Resources and Environment, Northwest A&F University, Yangling, Shaanxi 712100, China.
authors.forename: Manli
authors.initials: M
authors.lastname: Duan
authors.affiliation: College of Natural Resources and Environment, Northwest A&F University, Yangling, Shaanxi 712100, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Sun
authors.affiliation: College of Natural Resources and Environment, Northwest A&F University, Yangling, Shaanxi 712100, China.
authors.forename: Yajun
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: College of Natural Resources and Environment, Northwest A&F University, Yangling, Shaanxi 712100, China.
authors.forename: Haichao
authors.initials: H
authors.lastname: Li
authors.affiliation: College of Natural Resources and Environment, Northwest A&F University, Yangling, Shaanxi 712100, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Li
mesh_terms: D000693:Anaerobiosis; D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics; D004352:Drug Resistance, Microbial / Q000235:genetics*; D019426:Integrases / Q000235:genetics; D008372:Manure / Q000382:microbiology*; D013410:Sulfachlorpyridazine / Q000494:pharmacology*; D013449:Sulfonamides / Q000494:pharmacology*; D013552:Swine; D014865:Waste Disposal, Fluid / Q000379:methods; D015032:Zinc / Q000494:pharmacology*
chemical_list: D001426:Bacterial Proteins; D008372:Manure; D013449:Sulfonamides; D019426:Integrases; D015032:Zinc; D013410:Sulfachlorpyridazine
keywords: Antibiotic resistance gene; Mesophilic anaerobic digestion; Sulfachloropyridazine sodium; Zinc