title: Culture-Based Screening of Aerobic Microbiome in Diabetic Foot Subjects and Developing Non-healing Ulcers.
abstract: The study was carried on diabetic foot patients to deduce clinical attributes, the occurrence of the range of aerobic microbial flora and to assess their comparativein vitrosusceptibility to the customarily used antimicrobials. We also studied the potential risk factors involved in the development of non-healing ulcers. A total of 87 organisms were isolated from 70 specimens, includingEscherichia coli(19.5%) among the Gram-negative andStaphylococcus aureus(18.4%) among the Gram-positive as the predominant aerobes explored.Pseudomonas aeruginosaandE. coliwere predominant isolates of non-healing ulcers. The antimicrobial sensitivity pattern revealed that vancomycin (100%) and amikacin (90.4%) exhibited highest sensitivity to Gram-positive cocci, while all strains ofP. aeruginosawere sensitive toward imipenem (100%). The prevalent uncontrolled glycemic status, altered lipid spectra, the existence of neuropathy, and peripheral vascular disease, suggested predisposition toward the development of non-healing lesions. The study has underlined the need for continuous surveillance of bacteria and their antimicrobial sensitivity blueprints to provide the basis for empirical therapy and to minimize the risk of complications. Further, stringent clinical evaluation, and medical history will help in revealing the risk of developing non-healing status in diabetic foot ulcers.
authors.affiliation: Rajiv Gandhi Centre for Diabetes and Endocrinology, J.N Medical College, Aligarh Muslim University Aligarh, India.
authors.forename: Saba
authors.initials: S
authors.lastname: Noor
authors.affiliation: Rajiv Gandhi Centre for Diabetes and Endocrinology, J.N Medical College, Aligarh Muslim University Aligarh, India.
authors.forename: Jamal
authors.initials: J
authors.lastname: Ahmad
authors.affiliation: Department of Zoology, Faculty of Life Sciences, Aligarh Muslim University Aligarh, India.
authors.forename: Iqbal
authors.initials: I
authors.lastname: Parwez
authors.affiliation: Rajiv Gandhi Centre for Diabetes and Endocrinology, J.N Medical College, Aligarh Muslim University Aligarh, India.
authors.forename: Maaz
authors.initials: M
authors.lastname: Ozair
keywords: Antibiogram; Diabetic foot infection; Multi-drug resistant organisms; Non-healing ulcers; Type 2 diabetes