title: Datasets for the validation of the "in vivo" siRNA-silencing ofCD40and for the detection of new markers of atherosclerosis progression in ApoE-deficient mice.
abstract: Data presented in this Data in Brief article correspond to the article "in vivo" silencing ofCD40reduces progression of experimental atherogenesis through a NFκB/miR-125b axis and reveals new potential mediators in the pathogenesis of atherosclerosis" (M. Hueso, L. De Ramon, E. Navarro, E. Ripoll, J.M. Cruzado, J.M. Grinyo, J. Torras, 2016) [1]. Here, we describe the validation of the silencing ofCD40expression with a specific siRNA in ApoE-/-mouse aortas, and its systemic effects on splenic lymphocytic subpopulations as well as on the infiltration of aortic intima by F4/80+, galectin-3+macrophages or by NF-κB+cells. We also show the output of a Gene Ontology and TLDA analysis which allowed the detection of potential mediators of atherosclerosis progression. We provide the scientific community with a set of genes whose expression is increased during atherosclerosis progression but downregulated uponCD40silencing.
authors.affiliation: Department of Nephrology, Hospital Universitari Bellvitge, and Bellvitge Research Institute (IDIBELL), L'Hospitalet de Llobregat, Spain.
authors.forename: Miguel
authors.initials: M
authors.lastname: Hueso
authors.affiliation: Laboratory of Experimental Nephrology, Bellvitge Research Institute (IDIBELL), L'Hospitalet de Llobregat, Spain.
authors.forename: Laura
authors.initials: L
authors.lastname: De Ramon
authors.affiliation: Laboratory of Molecular Oncology, Bellvitge Research Institute (IDIBELL), L'Hospitalet de Llobregat, Spain.
authors.forename: Estanislao
authors.initials: E
authors.lastname: Navarro
authors.affiliation: Laboratory of Experimental Nephrology, Bellvitge Research Institute (IDIBELL), L'Hospitalet de Llobregat, Spain.
authors.forename: Elia
authors.initials: E
authors.lastname: Ripoll
authors.affiliation: Department of Nephrology, Hospital Universitari Bellvitge, and Bellvitge Research Institute (IDIBELL), L'Hospitalet de Llobregat, Spain.
authors.forename: Josep M
authors.initials: JM
authors.lastname: Cruzado
authors.affiliation: Department of Nephrology, Hospital Universitari Bellvitge, and Bellvitge Research Institute (IDIBELL), L'Hospitalet de Llobregat, Spain.
authors.forename: Josep M
authors.initials: JM
authors.lastname: Grinyo
authors.affiliation: Department of Nephrology, Hospital Universitari Bellvitge, and Bellvitge Research Institute (IDIBELL), L'Hospitalet de Llobregat, Spain.
authors.forename: Joan
authors.initials: J
authors.lastname: Torras
keywords: Atherosclerosis; CD40; Clec/Klr; GO analysis; Macrophages; NF-kB; miR-125b; siRNA