title: Molecular cloning and expression analysis of five heat shock protein 70 (HSP70) family members in Lateolabrax maculatus with Vibrio harveyi infection.
abstract: Heat shock proteins 70 (HSP70s) are molecular chaperones that aid in protection against environmental stress. In this study, we cloned and characterized five members of the HSP70 family (designated as HSPa1a, HSC70-1, HSC70-2, HSPa4 and HSPa14) from Lateolabrax maculatus using rapid amplification cDNA ends (RACE). Multiple sequence alignment and structural analysis revealed that all members of the HSP70 family had a conserved domain architecture, with some distinguishing features unique to each HSP70. Quantitative real-time (qPCR) analysis revealed that all members of the HSP70 family were ubiquitously and differentially expressed in all major types of tissues, including testicular tissue. This indicated that HSP70s have vital and conserved biological functions, and may also function in the development of germinal cells. The expression of mRNA of the five HSP70 family members mRNA expression was significantly increased in the head kidney, intestine and gill after Vibrio harveyi challenge, suggesting that HSP70s play an important role in the immune response.
authors.affiliation: Key Laboratory of Applied Marine Biotechnology by the Ministry of Education, Ningbo University, Ningbo, Zhejiang 315211, People's Republic of China.
authors.forename: Ying-Li
authors.initials: YL
authors.lastname: Han
authors.affiliation: Key Laboratory of Applied Marine Biotechnology by the Ministry of Education, Ningbo University, Ningbo, Zhejiang 315211, People's Republic of China.
authors.forename: Cong-Cong
authors.initials: CC
authors.lastname: Hou
authors.affiliation: Key Laboratory of Applied Marine Biotechnology by the Ministry of Education, Ningbo University, Ningbo, Zhejiang 315211, People's Republic of China.
authors.forename: Chen
authors.initials: C
authors.lastname: Du
authors.affiliation: Key Laboratory of Applied Marine Biotechnology by the Ministry of Education, Ningbo University, Ningbo, Zhejiang 315211, People's Republic of China. Electronic address: zhujunquan@nbu.edu.cn.
authors.forename: Jun-Quan
authors.initials: JQ
authors.lastname: Zhu
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics / Q000378:metabolism; D005393:Fish Diseases / Q000235:genetics* / Q000276:immunology; D029941:Fish Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D020869:Gene Expression Profiling; D018840:HSP70 Heat-Shock Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D008297:Male; D009928:Organ Specificity; D010473:Perciformes; D010802:Phylogeny; D011487:Protein Conformation; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction / Q000662:veterinary; D016415:Sequence Alignment / Q000662:veterinary; D014733:Vibrio / Q000502:physiology; D014735:Vibrio Infections / Q000235:genetics / Q000276:immunology / Q000662:veterinary*
chemical_list: D018076:DNA, Complementary; D029941:Fish Proteins; D018840:HSP70 Heat-Shock Proteins; D012333:RNA, Messenger
keywords: Expression pattern; HSP70; Immune response; Lateolabrax maculatus; Vibrio harveyi