title: Ligating Dopamine as Signal Trigger onto the Substrate via Metal-Catalyst-Free Click Chemistry for "Signal-On" Photoelectrochemical Sensing of Ultralow MicroRNA Levels.
abstract: The efficiency of photon-to-electron conversion is extremely restricted by the electron-hole recombinant. Here, a new photoelectrochemical (PEC) sensing platform has been established based on the signal amplification of click chemistry (CC) via hybridization chain reaction (HCR) for highly sensitive microRNA (miRNA) assay. In this proposal, a preferred electron donor dopamine (DA) was first assembled with designed ligation probe (probe-N3) via amidation reaction to achieve DA-coordinated signal probe (PDA-N3). The PDA-N3served as a flexible trigger to signal amplification through efficiently suppressing the electron-hole recombinant. Specifically, the PDA-N3can be successfully ligated into the trapped hairpins (H1 and H2) via the superior ligation method of metal-catalyst-free CC, in which the electron donor DA was introduced into the assay system. Moreover, the enzyme-free HCR, employed as a versatile amplification way, ensures that lots of PDA-N3can be attached to the substrate. This PEC sensing for miRNA-141 detection illustrated the outstanding linear response to a concentration variation from 0.1 fM to 0.5 nM and a detection limit down to 27 aM, without additional electron donors. The sensor is further employed to monitor miRNA-141 from prostate carcinoma cell (22Rv1), showing good quantitative detection capability. This strategy exquisitely influences the analytical performance and offers a new PEC route to highly selective and sensitive detection of biological molecules.
authors.affiliation: Key Laboratory of Eco-Environments in Three Gorges Reservoir Region (Ministry of Education), School of Chemistry and Chemical Engineering, Southwest University , Chongqing 400715, People's Republic of China.
authors.forename: Cui
authors.initials: C
authors.lastname: Ye
authors.affiliation: Institute for Clean Energy & Advanced Materials, Faculty of Materials and Energy, Southwest University , Chongqing 400715, People's Republic of China.
authors.forename: Min Qiang
authors.initials: MQ
authors.lastname: Wang
authors.affiliation: Key Laboratory of Eco-Environments in Three Gorges Reservoir Region (Ministry of Education), School of Chemistry and Chemical Engineering, Southwest University , Chongqing 400715, People's Republic of China.
authors.forename: Zhong Feng
authors.initials: ZF
authors.lastname: Gao
authors.affiliation: Key Laboratory of Eco-Environments in Three Gorges Reservoir Region (Ministry of Education), School of Chemistry and Chemical Engineering, Southwest University , Chongqing 400715, People's Republic of China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: School of Chemistry and Chemical Engineering, Chongqing University , Chongqing 400044, People's Republic of China.
authors.forename: Jing Lei
authors.initials: JL
authors.lastname: Lei
authors.affiliation: Key Laboratory of Eco-Environments in Three Gorges Reservoir Region (Ministry of Education), School of Chemistry and Chemical Engineering, Southwest University , Chongqing 400715, People's Republic of China.
authors.forename: Hong Qun
authors.initials: HQ
authors.lastname: Luo
authors.affiliation: Key Laboratory of Eco-Environments in Three Gorges Reservoir Region (Ministry of Education), School of Chemistry and Chemical Engineering, Southwest University , Chongqing 400715, People's Republic of China.
authors.forename: Nian Bing
authors.initials: NB
authors.lastname: Li
mesh_terms: D015374:Biosensing Techniques; D001729:Bismuth / Q000737:chemistry*; D045744:Cell Line, Tumor; D057930:Click Chemistry; D004220:Disulfides / Q000737:chemistry*; D004298:Dopamine / Q000737:chemistry*; D055664:Electrochemical Techniques; D006367:HeLa Cells; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000032:analysis*; D008982:Molybdenum / Q000737:chemistry*; D049329:Nanostructures / Q000737:chemistry; D021141:Nucleic Acid Amplification Techniques; D010316:Particle Size; D055668:Photochemical Processes; D011471:Prostatic Neoplasms / Q000737:chemistry*; D013440:Sulfides / Q000737:chemistry*; D013499:Surface Properties
chemical_list: D004220:Disulfides; C533895:MIRN141 microRNA, human; D035683:MicroRNAs; D013440:Sulfides; D008982:Molybdenum; D001729:Bismuth; D004298:Dopamine; C049897:bismuth sulfide; C082964:molybdenum disulfide