title: Detection of manganese peroxidase and other exoenzymes in four isolates of Geastrum (Geastrales) in pure culture.
abstract: Knowledge regarding the enzymatic machinery of fungi is decisive to understand their ecological role. The species of the genus Geastrum are known to grow extremely slowly in pure culture, which makes it difficult to evaluate physiological parameters such as enzyme activity. Qualitative assays were performed on isolates of four species of this genus, showing evidence of laccase, cellulase, pectinase, amylase and lipase activity and suggesting that a wide range of carbon sources can be exploited by these species. For the first time in this genus, quantitative assays verified manganese peroxidase activity (up to 0.6mU/g) in 30-day old cultures, as well as laccase, β-glycosidase and β-xylosidase activities.
authors.affiliation: Centro de Investigación y Extensión Forestal Andino Patagónico - CONICET, Argentina. Electronic address: fkuhar@gmail.com.
authors.forename: Francisco
authors.initials: F
authors.lastname: Kuhar
authors.affiliation: Laboratorio de Micología Experimental, Departamento de Biodiversidad y Biología Experimental, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Argentina.
authors.forename: Valeria C
authors.initials: VC
authors.lastname: Castiglia
authors.affiliation: Departamento de Micología, Real Jardín Botánico, CSIC, Madrid, Spain.
authors.forename: Juan C
authors.initials: JC
authors.lastname: Zamora
mesh_terms: D000681:Amylases / Q000032:analysis; D001487:Basidiomycota / Q000201:enzymology* / Q000254:growth & development; D002244:Carbon / Q000378:metabolism; D002480:Cellulase / Q000032:analysis; D003470:Culture Media; D005656:Fungal Proteins / Q000032:analysis*; D042845:Laccase / Q000032:analysis; D008049:Lipase / Q000032:analysis; D010544:Peroxidases / Q000032:analysis*; D011096:Polygalacturonase / Q000032:analysis; D013045:Species Specificity
chemical_list: D003470:Culture Media; D005656:Fungal Proteins; D002244:Carbon; D042845:Laccase; D010544:Peroxidases; C051129:manganese peroxidase; D008049:Lipase; D000681:Amylases; D011096:Polygalacturonase; D002480:Cellulase
keywords: Cellulase; Celulasa; Degradación de hojarasca; Lacasas; Laccase; Lipasa; Lipase; Litter degradation; Xilanasa; Xylanase