title: Fluorescent Oligonucleotide Probes for Screening High-Affinity Nucleobase Surrogates.
abstract: Double-helical oligonucleotide probes featuring a single-nucleotide gap opposed by one of the canonical nucleobases and flanked by the fluorescent nucleobase analogue pyrrolocytosine have been synthesized and titrated with PdIIchelates of dipicolinamide and its N2,N6-dialkylated derivatives. The fluorometric titrations revealed greatly increased affinity of the PdIIchelates for the nucleobases opposing the gap compared to the respective free nucleotides in solution. Owing to the constrained environment of the single-nucleotide gap, the relative stabilities of the various PdII-mediated base pairs were also significantly different from those previously reported at monomer level.
authors.affiliation: Department of Chemistry, University of Turku, Vatselankatu 2, 20014, Turku, Finland.
authors.forename: Asmo
authors.initials: A
authors.lastname: Aro-Heinilä
authors.affiliation: Department of Chemistry, University of Turku, Vatselankatu 2, 20014, Turku, Finland.
authors.forename: Tuomas
authors.identifier: http://orcid.org/0000-0003-3607-3116
authors.initials: T
authors.lastname: Lönnberg
mesh_terms: D000577:Amides / Q000737:chemistry; D020029:Base Pairing; D002614:Chelating Agents / Q000737:chemistry; D005456:Fluorescent Dyes / Q000737:chemistry*; D015345:Oligonucleotide Probes / Q000737:chemistry*; D010165:Palladium / Q000737:chemistry; D010848:Picolinic Acids / Q000737:chemistry
chemical_list: D000577:Amides; D002614:Chelating Agents; D005456:Fluorescent Dyes; D015345:Oligonucleotide Probes; D010848:Picolinic Acids; D010165:Palladium; C003142:picolinamide
keywords: base pairs; chelates; fluorescence; oligonucleotide; palladium