title: Early discrimination of nasopharyngeal carcinoma based on tissue deoxyribose nucleic acid surface-enhanced Raman spectroscopy analysis.
abstract: Surface-enhanced Raman spectroscopy (SERS) was employed to detect deoxyribose nucleic acid (DNA) variations associated with the development of nasopharyngeal carcinoma (NPC). Significant SERS spectral differences between the DNA extracted from early NPC, advanced NPC, and normal nasopharyngeal tissue specimens were observed at 678, 729, 788, 1337, 1421, 1506, and 1573??cm?1, which reflects the genetic variations in NPC. Principal component analysis combined with discriminant function analysis for early NPC discrimination yielded a diagnostic accuracy of 86.8%, 92.3%, and 87.9% for early NPC, advanced NPC, and normal nasopharyngeal tissue DNA, respectively. In this exploratory study, we demonstrated the potential of SERS for early detection of NPC based on the DNA molecular study of biopsy tissues.
authors.affiliation: Fujian Cancer Hospital, Fujian Medical University Cancer Hospital, Department of Radiation Oncology, No. 420, Fuma Road, Fuzhou 350014, Fujian, ChinabFujian Provincial Key Laboratory of Translational Cancer Medicine, Fuzhou, Fujian 350122, China.
authors.forename: Sufang
authors.initials: S
authors.lastname: Qiu
authors.affiliation: The Shengli Clinical College of Fujian Medical University, Fuzhou, Fujian 350001, China.
authors.forename: Chao
authors.initials: C
authors.lastname: Li
authors.affiliation: Fujian Cancer Hospital, Fujian Medical University Cancer Hospital, Department of Radiation Oncology, No. 420, Fuma Road, Fuzhou 350014, Fujian, China.
authors.forename: Jinyong
authors.initials: J
authors.lastname: Lin
authors.affiliation: The Shengli Clinical College of Fujian Medical University, Fuzhou, Fujian 350001, China.
authors.forename: Yuanji
authors.initials: Y
authors.lastname: Xu
authors.affiliation: Fujian Cancer Hospital, Fujian Medical University Cancer Hospital, Department of Radiation Oncology, No. 420, Fuma Road, Fuzhou 350014, Fujian, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Lu
authors.affiliation: Fujian Cancer Hospital, Fujian Medical University Cancer Hospital, Department of Radiation Oncology, No. 420, Fuma Road, Fuzhou 350014, Fujian, China.
authors.forename: Qingting
authors.initials: Q
authors.lastname: Huang
authors.affiliation: Fujian Provincial Cancer Hospital, Fujian Medical University Cancer Hospital, Department of Radiation Biological Laboratory, Fuzhou, Fujian 350014, China.
authors.forename: Changyan
authors.initials: C
authors.lastname: Zou
authors.affiliation: Fujian Provincial Cancer Hospital, Fujian Medical University Cancer Hospital, Department of Radiation Biological Laboratory, Fuzhou, Fujian 350014, China.
authors.forename: Chao
authors.initials: C
authors.lastname: Chen
authors.affiliation: Fujian Normal University, The Key Laboratories of Innate Immune Biology of Fujian Province, Southern Biomedical Research Center, Fuzhou 350007, China.
authors.forename: Nanyang
authors.initials: N
authors.lastname: Xiao
authors.affiliation: Fujian Normal University, Fujian Provincial Key Laboratory for Photonics Technology, Key Laboratory of Optoelectronic Science and Technology for Medicine, Ministry of Education, Fuzhou 350007, ChinagFujian University of Traditional Chinese Medicine, College of Integrated Traditional Chinese and Western Medicine, Fuzhou 350108, China.
authors.forename: Duo
authors.initials: D
authors.lastname: Lin
authors.affiliation: Fujian Normal University, Fujian Provincial Key Laboratory for Photonics Technology, Key Laboratory of Optoelectronic Science and Technology for Medicine, Ministry of Education, Fuzhou 350007, China.
authors.forename: Rong
authors.initials: R
authors.lastname: Chen
authors.affiliation: Fujian Cancer Hospital, Fujian Medical University Cancer Hospital, Department of Radiation Oncology, No. 420, Fuma Road, Fuzhou 350014, Fujian, ChinabFujian Provincial Key Laboratory of Translational Cancer Medicine, Fuzhou, Fujian 350122, China.
authors.forename: Jianji
authors.initials: J
authors.lastname: Pan
authors.affiliation: Fujian Normal University, Fujian Provincial Key Laboratory for Photonics Technology, Key Laboratory of Optoelectronic Science and Technology for Medicine, Ministry of Education, Fuzhou 350007, China.
authors.forename: Shangyuan
authors.initials: S
authors.lastname: Feng
mesh_terms: D000465:Algorithms; D002277:Carcinoma / Q000737:chemistry / Q000000981:diagnostic imaging*; D004247:DNA / Q000032:analysis* / Q000737:chemistry; D006801:Humans; D007090:Image Interpretation, Computer-Assisted / Q000379:methods*; D008875:Middle Aged; D000077274:Nasopharyngeal Carcinoma; D009303:Nasopharyngeal Neoplasms / Q000737:chemistry / Q000000981:diagnostic imaging*; D025341:Principal Component Analysis; D012834:Silver / Q000737:chemistry; D013059:Spectrum Analysis, Raman / Q000379:methods*
chemical_list: C586932:colloidal silver; D012834:Silver; D004247:DNA