title: Consumption of the total Western diet differentially affects the response to green tea in rodent models of chronic disease compared to the AIN93G diet.
abstract: In pre-clinical studies investigating bioactive components, the efficacy of the bioactive is likely influenced by the basal diet provided to rodents. In this study, we hypothesized that a model bioactive, green tea extract (GTE), would have different effects on colon carcinogenesis, body composition, and lipid metabolism in mice fed a basal diet formulated to promote animal health and growth (AIN93G) as compared to a Western diet that emulates typical American intakes of micro- and macronutrients, the total Western diet (TWD).
  
  
  
  Mice were fed either AIN93G or TWD, with or without GTE added to drinking water for 18 weeks. Aberrant crypt foci (ACF) in azoxymethane-initiated mice was nearly three times greater in mice fed TWD compared to AIN93G. Consumption of GTE suppressed ACF development only in mice fed the TWD. Similarly, supplementation with GTE suppressed weight gain and fasted glucose only in mice fed TWD, while GTE suppressed fat mass in mice fed either diet. Irrespective of diet, GTE supplementation increased cecum weight and decreased cecal SCFA concentration.
  
  
  
  Collectively, these observations indicate that the TWD influences the bioactivity of GTE in rodent models of obesity, metabolism, and carcinogenesis.
authors.affiliation: Department of Nutrition, Dietetics and Food Sciences, Utah State University, Logan, UT, USA.|USTAR Applied Nutrition Research, Utah State University, Logan, UT, USA.
authors.forename: Robert E
authors.initials: RE
authors.lastname: Ward
authors.affiliation: USTAR Applied Nutrition Research, Utah State University, Logan, UT, USA.|Department of Animal, Dairy and Veterinary Sciences, Utah State University, Logan, UT, USA.|School of Veterinary Medicine, Utah State University, Logan, UT, USA.
authors.forename: Abby D
authors.initials: AD
authors.lastname: Benninghoff
authors.affiliation: Department of Nutrition, Dietetics and Food Sciences, Utah State University, Logan, UT, USA.
authors.forename: Brett J
authors.initials: BJ
authors.lastname: Healy
authors.affiliation: Department of Nutrition, Dietetics and Food Sciences, Utah State University, Logan, UT, USA.
authors.forename: Minghao
authors.initials: M
authors.lastname: Li
authors.affiliation: Department of Nutrition, Dietetics and Food Sciences, Utah State University, Logan, UT, USA.
authors.forename: Bharath
authors.initials: B
authors.lastname: Vagu
authors.affiliation: Department of Nutrition, Dietetics and Food Sciences, Utah State University, Logan, UT, USA.|USTAR Applied Nutrition Research, Utah State University, Logan, UT, USA.
authors.forename: Korry J
authors.initials: KJ
authors.lastname: Hintze
mesh_terms: D000818:Animals; D001397:Azoxymethane; D001786:Blood Glucose / Q000032:analysis; D001835:Body Weight; D066273:Diet, Western; D004041:Dietary Fats / Q000378:metabolism; D005947:Glucose; D050356:Lipid Metabolism; D008099:Liver / Q000378:metabolism; D008297:Male; D051379:Mice; D023421:Models, Animal; D009765:Obesity / Q000378:metabolism; D013662:Tea / Q000737:chemistry*
chemical_list: D001786:Blood Glucose; D004041:Dietary Fats; D013662:Tea; D005947:Glucose; D001397:Azoxymethane
keywords: Colon cancer; Green tea; Lipid metabolism; Short chain fatty acids; Total Western diet