title: Capping Protein Modulates Actin Remodeling in Response to Reactive Oxygen Species during Plant Innate Immunity.
abstract: Plants perceive microbe-associated molecular patterns and damage-associated molecular patterns to activate innate immune signaling events, such as bursts of reactive oxygen species (ROS). The actin cytoskeleton remodels during the first 5 min of innate immune signaling in Arabidopsis (Arabidopsis thaliana) epidermal cells; however, the immune signals that impinge on actin cytoskeleton and its response regulators remain largely unknown. Here, we demonstrate that rapid actin remodeling upon elicitation with diverse microbe-associated molecular patterns and damage-associated molecular patterns represent a conserved plant immune response. Actin remodeling requires ROS generated by the defense-associated NADPH oxidase, RBOHD. Moreover, perception of flg22 by its cognate receptor complex triggers actin remodeling through the activation of RBOHD-dependent ROS production. Our genetic studies reveal that the ubiquitous heterodimeric capping protein transduces ROS signaling to the actin cytoskeleton during innate immunity. Additionally, we uncover a negative feedback loop between actin remodeling and flg22-induced ROS production.
authors.affiliation: Department of Biological Sciences, Purdue University, West Lafayette, Indiana 47907-2064 (J.L., L.C., C.J.S.); and.|The Bindley Bioscience Center and Department of Botany and Plant Pathology, Purdue University, West Lafayette, Indiana 47907 (C.J.S.).
authors.forename: Jiejie
authors.identifier: 0000-0002-2906-1566
authors.initials: J
authors.lastname: Li
authors.affiliation: Department of Biological Sciences, Purdue University, West Lafayette, Indiana 47907-2064 (J.L., L.C., C.J.S.); and.|The Bindley Bioscience Center and Department of Botany and Plant Pathology, Purdue University, West Lafayette, Indiana 47907 (C.J.S.).
authors.forename: Lingyan
authors.initials: L
authors.lastname: Cao
authors.affiliation: Department of Biological Sciences, Purdue University, West Lafayette, Indiana 47907-2064 (J.L., L.C., C.J.S.); and staiger@purdue.edu.|The Bindley Bioscience Center and Department of Botany and Plant Pathology, Purdue University, West Lafayette, Indiana 47907 (C.J.S.) staiger@purdue.edu.
authors.forename: Christopher J
authors.identifier: 0000-0003-2321-1671
authors.initials: CJ
authors.lastname: Staiger
mesh_terms: D051344:Actin Capping Proteins / Q000378:metabolism*; D008841:Actin Cytoskeleton / Q000187:drug effects / Q000378:metabolism; D000199:Actins / Q000378:metabolism*; D000067531:Alarmins; D017360:Arabidopsis / Q000187:drug effects / Q000276:immunology*; D029681:Arabidopsis Proteins / Q000378:metabolism*; D001713:Biphenyl Compounds / Q000494:pharmacology; D005408:Flagellin / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000494:pharmacology; D007113:Immunity, Innate / Q000187:drug effects*; D019255:NADPH Oxidases / Q000378:metabolism; D009861:Onium Compounds / Q000494:pharmacology; D000069452:Pathogen-Associated Molecular Pattern Molecules / Q000378:metabolism; D057865:Plant Immunity / Q000187:drug effects; D017382:Reactive Oxygen Species / Q000378:metabolism*; D015398:Signal Transduction / Q000187:drug effects
chemical_list: D051344:Actin Capping Proteins; D000199:Actins; D000067531:Alarmins; D029681:Arabidopsis Proteins; D001713:Biphenyl Compounds; D009861:Onium Compounds; D000069452:Pathogen-Associated Molecular Pattern Molecules; D017382:Reactive Oxygen Species; C031291:diphenyliodonium; D005408:Flagellin; D006861:Hydrogen Peroxide; D019255:NADPH Oxidases