title: Selective inactivation of enzymes conjugated to nanoparticles using tuned laser illumination.
abstract: We report a novel method for specific deactivation of conjugated enzymes using laser-heated gold nanoparticles. Current methods involve treatment of the entire solution, thereby inactivating all bioactive components. Our method enables inactivation of only a single or subset of targeted enzymes. The selected enzyme is pre-conjugated to gold nanoparticles, which are specifically heated by a laser tuned to their surface plasmon resonance. We demonstrate inactivation of a selected enzyme, glucose oxidase, within a mixture of biomolecules. Illumination of non-conjugated enzymes and nanoparticles demonstrated specificity. We propose a novel method to quantitatively regulate enzyme activity, providing a building block for cellular and cell-free biochemical reactions. © 2016 International Society for Advancement of Cytometry.
authors.affiliation: Faculty of Engineering, Bar Ilan University, Ramat-Gan, 5290002, Israel.|The Bar-Ilan Institute of Nanotechnologies & Advanced Materials, Bar Ilan University, Ramat-Gan, 5290002, Israel.
authors.forename: Asaf
authors.initials: A
authors.lastname: Ilovitsh
authors.affiliation: Faculty of Engineering, Bar Ilan University, Ramat-Gan, 5290002, Israel.|The Bar-Ilan Institute of Nanotechnologies & Advanced Materials, Bar Ilan University, Ramat-Gan, 5290002, Israel.
authors.forename: Pazit
authors.initials: P
authors.lastname: Polak
authors.affiliation: Faculty of Engineering, Bar Ilan University, Ramat-Gan, 5290002, Israel.|The Bar-Ilan Institute of Nanotechnologies & Advanced Materials, Bar Ilan University, Ramat-Gan, 5290002, Israel.
authors.forename: Zeev
authors.initials: Z
authors.lastname: Zalevsky
authors.affiliation: Faculty of Engineering, Bar Ilan University, Ramat-Gan, 5290002, Israel.|The Bar-Ilan Institute of Nanotechnologies & Advanced Materials, Bar Ilan University, Ramat-Gan, 5290002, Israel.
authors.forename: Orit
authors.initials: O
authors.lastname: Shefi
mesh_terms: D005949:Glucose Oxidase / Q000737:chemistry*; D006046:Gold / Q000737:chemistry; D007834:Lasers; D053768:Metal Nanoparticles / Q000737:chemistry*; D020349:Surface Plasmon Resonance / Q000379:methods
chemical_list: D006046:Gold; D005949:Glucose Oxidase
keywords: enzyme inactivation; laser heating; nanoparticles