title: Isolation and Characterization ofAcinetobacter baumanniiRecovered fromCampylobacterSelective Medium.
abstract: Acinetobacter baumannii, a Gram-negative opportunistic pathogen, is known to cause multidrug resistant infections. This organism has primarily been isolated from clinical environments and its environmental reservoirs remain largely unknown. In the present study, we recovered seven isolates ofA. baumanniigrowing under conditions selective forCampylobacterspp. (microaerophilic at 42°C and in the presence of antibiotics) from dairy cattle manure storage tank or surface water impacted by livestock effluents. Antibiotic susceptibility tests revealed that all of these isolates were less susceptible to at least two different clinically relevant antibiotics, compared to the type strainA. baumanniiATCC17978. Expression of resistance-nodulation-division efflux pumps, an important mechanism of intrinsic resistance in these organisms, was analyzed, andadeBwas found to be overexpressed in one andadeJwas overexpressed in three isolates. Comparison of these isolates using genomic DNA Macro-Restriction Fragment Pattern Analysis (MRFPA) revealed relatively low relatedness among themselves or with some of the clinical isolates from previous studies. This study suggests thatA. baumanniiisolates are capable of growing under selective conditions forCampylobacterspp. and that this organism can be present in manure and water.
authors.affiliation: Department of Microbiology, University of Manitoba , Winnipeg, MB, Canada.
authors.forename: Dinesh M
authors.initials: DM
authors.lastname: Fernando
authors.affiliation: Ottawa Research and Development Centre, Agriculture and Agri-Food Canada , Ottawa, ON, Canada.
authors.forename: Izhar U H
authors.initials: IU
authors.lastname: Khan
authors.affiliation: Department of Microbiology, University of Manitoba , Winnipeg, MB, Canada.
authors.forename: Rakesh
authors.initials: R
authors.lastname: Patidar
authors.affiliation: Ottawa Research and Development Centre, Agriculture and Agri-Food Canada , Ottawa, ON, Canada.
authors.forename: David R
authors.initials: DR
authors.lastname: Lapen
authors.affiliation: Sherbrooke Research and Development Centre, Agriculture and Agri-Food Canada , Sherbrooke, QC, Canada.
authors.forename: Guylaine
authors.initials: G
authors.lastname: Talbot
authors.affiliation: London Research and Development Centre, Agriculture and Agri-Food Canada , London, ON, Canada.
authors.forename: Edward
authors.initials: E
authors.lastname: Topp
authors.affiliation: Department of Microbiology, University of Manitoba, Winnipeg, MB, Canada; Department of Medical Microbiology, University of Manitoba, Winnipeg, MB, Canada.
authors.forename: Ayush
authors.initials: A
authors.lastname: Kumar
keywords: MRFPA; RND pumps; agriculture niche; antibiotic susceptibility; microaerophilic