title: Sertoli cell condition medium can induce germ like cells from bone marrow derived mesenchymal stem cells.
abstract: OBJECTIVE
  Although many researchers have confirmed induction of germ cells from bone marrow mesenchymal stem cells (BMMSCs), there are no reports that confirm spontaneous differentiation of germ cells from BMMSCs. In this study, we have evaluated the effect of adult Sertoli cell condition medium (SCCM) as a mutative factor in the induction of germ cells from BMMSCs.
  
  
  METHODS
  BMMSCs were collected from the bone marrow of 6-8-week old NMRI mice and their mesenchymal entities were proven using superficial markers (expression of CD44 and CD73 and non-expresion of CD45 and CD11b) by fow cytometry. Their multi-potential entities were proved with differentiation to osteogenic and adipogenic cells for 21 days. Also isolated Sertoli cells were enriched using lectin coated plates and Sertoli cell condition medium (SCCM) was collected. Sertoli cells were identified by immunocytochemistry and Vimentin marker. The cells were then differentiated into germ cells with SCCM for 2 weeks. Finally induced cells were evaluated by RT-PCR and immunocytochemistry.
  
  
  RESULTS
  Differentiation of mesenchymal stem cells to osteoblast and adipocyte showed their multi-potential property. Expression of CD44 and CD73 and non-expression of CD45 and CD11b confirmed mesenchyme cells. Immunocytochemistry and RT-PCR results showed expression of germ cells specific marker (Mvh).
  
  
  CONCLUSIONS
  This study confirmed the effect of SCCM as a motivational factor that can used for differentiation of germ cells from BMMSCs.
authors.affiliation: Department of Anatomical Sciences, School of Medicine, Tehran University of Medical Science, Tehran, Iran.
authors.forename: Mahdieh Hajian
authors.initials: MH
authors.lastname: Monfared
authors.affiliation: Department of Anatomical Sciences, School of Medicine, Tehran University of Medical Science, Tehran, Iran.
authors.forename: Bagher
authors.initials: B
authors.lastname: Minaee
authors.affiliation: Department of Anatomical Sciences, School of Medicine, Tehran University of Medical Science, Tehran, Iran.
authors.forename: Tayebeh
authors.initials: T
authors.lastname: Rastegar
authors.affiliation: Department of Anatomical Sciences, School of Medicine, Tehran University of Medical Science, Tehran, Iran.
authors.forename: Ebrahim
authors.initials: E
authors.lastname: Khrazinejad
authors.affiliation: Department of Anatomical Sciences, School of Medicine, Tehran University of Medical Science, Tehran, Iran.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Barbarestani
keywords: Bone marrow mesenchymal - stem cells; Differentiation; Germ cells; Sertoli cell condition medium