title: [CCR7 silence by siRNA inhibits proliferation, invasion and promotes apoptosis of human MG63 osteosarcoma cells].
abstract: Objective To investigate the effect of siRNA-mediated chemokine receptor 7 (CCR7) silence on the proliferation, migration, invasion and apoptosis of human MG-63 osteosarcoma cells. Methods The study designed and synthesized siRNA targeting CCR7 (CCR7-siRNA). After MG63 cells were transfected with CCR7-siRNA, the expression of CCR7 was identified by Western blotting; cell apoptosis was detected by annexinV-FITC/PI double staining combined with flow cemetery; cell proliferation was tested by MTT assay; and cell migration and invasion abilities were examined by TranswellTMmigration/invasion assays. Results CCR7 expression in MG63 cells was significantly inhibited after transfected with CCR7-siRNA. At the same time, cell proliferation, migration and invasion abilities were distinctly suppressed, and cell apoptosis rate increased. Conclusion Down-regulating CCR7 expression in MG63 cells could apparently inhibit cell proliferation, migration and invasion abilities of MG63 cells, and also induce cell apoptosis.
authors.affiliation: Orthopedic Surgery Center and Orthopedic Oncology Institute of PLA, Tangdu Hospital, Fourth Military Medical University, Xi'an 710038; Department of Traumatology, Huashan Central Hospital, Xi'an 710043, China.
authors.forename: Richun
authors.initials: R
authors.lastname: Zhang
authors.affiliation: Orthopedic Surgery Center and Orthopedic Oncology Institute of PLA, Tangdu Hospital, Fourth Military Medical University, Xi'an 710038, China.
authors.forename: Hongtao
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Department of Neurosurgery, Tangdu Hospital, Fourth Military Medical University, Xi'an 710038, China.
authors.forename: Zhen
authors.initials: Z
authors.lastname: E
authors.affiliation: Orthopedic Surgery Center and Orthopedic Oncology Institute of PLA, Tangdu Hospital, Fourth Military Medical University, Xi'an 710038, China.
authors.forename: Qiong
authors.initials: Q
authors.lastname: Ma
authors.affiliation: Orthopedic Surgery Center and Orthopedic Oncology Institute of PLA, Tangdu Hospital, Fourth Military Medical University, Xi'an 710038, China.
authors.forename: Shiju
authors.initials: S
authors.lastname: Yan
authors.affiliation: Orthopedic Surgery Center and Orthopedic Oncology Institute of PLA, Tangdu Hospital, Fourth Military Medical University, Xi'an 710038, China.
authors.forename: Enwei
authors.initials: E
authors.lastname: Zhang
authors.affiliation: Orthopedic Surgery Center and Orthopedic Oncology Institute of PLA, Tangdu Hospital, Fourth Military Medical University, Xi'an 710038, China. *Corresponding author, E-mail: gukemba@fmmu.edu.cn.
authors.forename: Bao'an
authors.initials: B
authors.lastname: Ma
mesh_terms: D017209:Apoptosis / Q000235:genetics / Q000502:physiology*; D015153:Blotting, Western; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics / Q000502:physiology; D049109:Cell Proliferation / Q000235:genetics / Q000502:physiology*; D020868:Gene Silencing / Q000502:physiology; D006801:Humans; D012516:Osteosarcoma / Q000378:metabolism* / Q000473:pathology; D034741:RNA, Small Interfering / Q000235:genetics; D054400:Receptors, CCR7 / Q000235:genetics / Q000378:metabolism*
chemical_list: C515370:CCR7 protein, human; D034741:RNA, Small Interfering; D054400:Receptors, CCR7