title: Preparation of Protein Conjugates via Homobifunctional Diselenoester Cross-Linker.
abstract: Adipic acid diselenoester was developed as an efficient cross-linker for covalent protein conjugation with a variety of small molecular haptens, including mono- and disaccharides, peptide, fluorescence dye, and nicotine. Compared to the counterparts of N-hydroxysuccinimide (NHS) and p-nitrophenyl (PNP) linkers, the diselenoester linker demonstrates improved balance between reactivity and stability and coupling of haptens to proteins under mild conditions with high incorporation efficiency.
authors.affiliation: Key Laboratory of Pesticide & Chemical Biology of Ministry of Education, CCNU-uOttawa Joint Research Centre, College of Chemistry, Central China Normal University , 152 Luoyu Road, Wuhan, Hubei 430079, P. R. China.
authors.forename: Xu-Guang
authors.initials: XG
authors.lastname: Yin
authors.affiliation: Key Laboratory of Pesticide & Chemical Biology of Ministry of Education, CCNU-uOttawa Joint Research Centre, College of Chemistry, Central China Normal University , 152 Luoyu Road, Wuhan, Hubei 430079, P. R. China.
authors.forename: Xiao-Fei
authors.initials: XF
authors.lastname: Gao
authors.affiliation: Key Laboratory of Pesticide & Chemical Biology of Ministry of Education, CCNU-uOttawa Joint Research Centre, College of Chemistry, Central China Normal University , 152 Luoyu Road, Wuhan, Hubei 430079, P. R. China.
authors.forename: Jing-Jing
authors.initials: JJ
authors.lastname: Du
authors.affiliation: Key Laboratory of Pesticide & Chemical Biology of Ministry of Education, CCNU-uOttawa Joint Research Centre, College of Chemistry, Central China Normal University , 152 Luoyu Road, Wuhan, Hubei 430079, P. R. China.
authors.forename: Xiao-Kang
authors.initials: XK
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Pesticide & Chemical Biology of Ministry of Education, CCNU-uOttawa Joint Research Centre, College of Chemistry, Central China Normal University , 152 Luoyu Road, Wuhan, Hubei 430079, P. R. China.
authors.forename: Xiang-Zhao
authors.initials: XZ
authors.lastname: Chen
authors.affiliation: Key Laboratory of Pesticide & Chemical Biology of Ministry of Education, CCNU-uOttawa Joint Research Centre, College of Chemistry, Central China Normal University , 152 Luoyu Road, Wuhan, Hubei 430079, P. R. China.
authors.forename: Jian
authors.initials: J
authors.lastname: Wang
authors.affiliation: Key Laboratory of Pesticide & Chemical Biology of Ministry of Education, CCNU-uOttawa Joint Research Centre, College of Chemistry, Central China Normal University , 152 Luoyu Road, Wuhan, Hubei 430079, P. R. China.
authors.forename: Ling-Ming
authors.initials: LM
authors.lastname: Xin
authors.affiliation: Key Laboratory of Pesticide & Chemical Biology of Ministry of Education, CCNU-uOttawa Joint Research Centre, College of Chemistry, Central China Normal University , 152 Luoyu Road, Wuhan, Hubei 430079, P. R. China.
authors.forename: Ze
authors.initials: Z
authors.lastname: Lei
authors.affiliation: Key Laboratory of Pesticide & Chemical Biology of Ministry of Education, CCNU-uOttawa Joint Research Centre, College of Chemistry, Central China Normal University , 152 Luoyu Road, Wuhan, Hubei 430079, P. R. China.
authors.forename: Zheng
authors.initials: Z
authors.lastname: Liu
authors.affiliation: Key Laboratory of Pesticide & Chemical Biology of Ministry of Education, CCNU-uOttawa Joint Research Centre, College of Chemistry, Central China Normal University , 152 Luoyu Road, Wuhan, Hubei 430079, P. R. China.
authors.forename: Jun
authors.initials: J
authors.lastname: Guo
mesh_terms: D000272:Adipates / Q000138:chemical synthesis / Q000737:chemistry*; D003432:Cross-Linking Reagents / Q000138:chemical synthesis / Q000737:chemistry*; D006241:Haptens / Q000737:chemistry*; D049673:History, 20th Century; D016566:Organoselenium Compounds / Q000138:chemical synthesis / Q000737:chemistry*; D010047:Ovalbumin / Q000737:chemistry*; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D014614:Vaccines, Synthetic / Q000737:chemistry
chemical_list: D000272:Adipates; D003432:Cross-Linking Reagents; D006241:Haptens; D016566:Organoselenium Compounds; D014614:Vaccines, Synthetic; D012710:Serum Albumin, Bovine; C029900:adipic acid; D010047:Ovalbumin