title: Curcumin Suppressed Activation of Dendritic Cells via JAK/STAT/SOCS Signal in Mice with Experimental Colitis.
abstract: Dendritic cells (DCs) play a pivotal role as initiators in the pathogenesis of inflammatory bowel disease and are regulated by the JAK/STAT/SOCS signaling pathway. As a potent anti-inflammatory compound, curcumin represents a viable treatment alternative or adjunctive therapy in the management of chronic inflammatory bowel disease (IBD). The mechanism of curcumin treated IBD on DCs is not completely understood. In the present study, we explored the mechanism of curcumin treated experimental colitis by observing activation of DCs via JAK/STAT/SOCS signaling pathway in colitis mice. Experimental colitis was induced by 2, 4, 6-trinitrobenzene sulfonic acid. After 7 days treatment with curcumin, its therapeutic effect was verified by decreased colonic weight, histological scores, and remitting pathological injury. Meanwhile, the levels of major histocompatibility complex class II and DC costimulatory molecules (CD83, CD28, B7-DC, CD40, CD40 L, and TLR2) were inhibited and followed the up-regulated levels of IL-4, IL-10, and IFN-γ, and down-regulated GM-CSF, IL-12p70, IL-15, IL-23, and TGF-β1. A key finding was that the phosphorylation of the three members (JAK2, STAT3, and STAT6) of the JAK/STAT/SOCS signaling pathway was inhibited, and the three downstream proteins (SOCS1, SOCS3, and PIAS3) from this pathway were highly expressed. In conclusion, curcumin suppressed the activation of DCs by modulating the JAK/STAT/SOCS signaling pathway to restore immunologic balance to effectively treat experimental colitis.
authors.affiliation: School of Basic Medical Sciences, Jiangxi University of Traditional Chinese Medicine Nanchang, China.
authors.forename: Hai-Mei
authors.initials: HM
authors.lastname: Zhao
authors.affiliation: Department of Postgraduate, Jiangxi University of Traditional Chinese Medicine Nanchang, China.
authors.forename: Rong
authors.initials: R
authors.lastname: Xu
authors.affiliation: Key Laboratory of Modern Preparation of TCM, Ministry of Education, Jiangxi University of Traditional Chinese Medicine Nanchang, China.
authors.forename: Xiao-Ying
authors.initials: XY
authors.lastname: Huang
authors.affiliation: School of Basic Medical Sciences, Jiangxi University of Traditional Chinese Medicine Nanchang, China.
authors.forename: Shao-Min
authors.initials: SM
authors.lastname: Cheng
authors.affiliation: Department of Postgraduate, Jiangxi University of Traditional Chinese Medicine Nanchang, China.
authors.forename: Min-Fang
authors.initials: MF
authors.lastname: Huang
authors.affiliation: Department of Postgraduate, Jiangxi University of Traditional Chinese Medicine Nanchang, China.
authors.forename: Hai-Yang
authors.initials: HY
authors.lastname: Yue
authors.affiliation: Department of Postgraduate, Jiangxi University of Traditional Chinese Medicine Nanchang, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Postgraduate, Jiangxi University of Traditional Chinese Medicine Nanchang, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Zou
authors.affiliation: School of Chinese Medicine, Hong Kong Baptist University Kowloon Tong, China.
authors.forename: Ai-Ping
authors.initials: AP
authors.lastname: Lu
authors.affiliation: Science and Technology College, Jiangxi University of Traditional Chinese Medicine Nanchang, China.
authors.forename: Duan-Yong
authors.initials: DY
authors.lastname: Liu
keywords: JAK/STAT/SOCS signal; costimulatory molecules; curcumin; dendritic cell; experimental colitis