title: A two-photon fluorescent probe for biological Cu (Ⅱ) and PPi detection in aqueous solution and in vivo.
abstract: The first two-photon fluorescent probe (PC) for selectively detecting biological Cu (Ⅱ) and pyrophosphate (PPi) has been developed based on 7-substituted coumarin in this study. The probe presented excellent selective two-photon "on-off-on" detection signal for Cu(II) /PPi in aqueous solution. The two-photon detection ensemble (PCCu) can detect PPi released from DNA amplification after the polymerase chain reactions (PCR). The probe showed low cytotoxicity and good biocompatibility, and therefore can be applied for imaging Cu(II)/PPi in living cells under two-photon excitation. Furthermore, the ensemble probe (PCCu) was also used to image PPi in deep living rat tissues (~100µm) and in 5-days old zebrafish.
authors.affiliation: Department of Chemistry, Anhui University, Hefei 230601, China.
authors.forename: Mengmeng
authors.initials: M
authors.lastname: Guo
authors.affiliation: Jiangsu Collaborative Innovation Center for Ecological Building Materials and Environmental Protection Equipments, Yancheng Institute of Technology, Yancheng 224051, China.
authors.forename: Pengyu
authors.initials: P
authors.lastname: Dong
authors.affiliation: Department of Chemistry, Anhui University, Hefei 230601, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Feng
authors.affiliation: Jiangsu Collaborative Innovation Center for Ecological Building Materials and Environmental Protection Equipments, Yancheng Institute of Technology, Yancheng 224051, China.
authors.forename: Xinguo
authors.initials: X
authors.lastname: Xi
authors.affiliation: Jiangsu Collaborative Innovation Center for Ecological Building Materials and Environmental Protection Equipments, Yancheng Institute of Technology, Yancheng 224051, China.
authors.forename: Rong
authors.initials: R
authors.lastname: Shao
authors.affiliation: School of life science, Anhui University, Hefei 230601, China.
authors.forename: Xiaohe
authors.initials: X
authors.lastname: Tian
authors.affiliation: Institute of Health Science, Anhui University, Hefei 230601, China.
authors.forename: Buchang
authors.initials: B
authors.lastname: Zhang
authors.affiliation: Department of Chemistry, Anhui University, Hefei 230601, China.
authors.forename: Manzhou
authors.initials: M
authors.lastname: Zhu
authors.affiliation: Department of Chemistry, Anhui University, Hefei 230601, China. Electronic address: mengxm@ahu.edu.cn.
authors.forename: Xiangming
authors.initials: X
authors.lastname: Meng
mesh_terms: D000818:Animals; D015374:Biosensing Techniques / Q000379:methods; D003300:Copper / Q000032:analysis*; D004247:DNA / Q000737:chemistry; D011756:Diphosphates / Q000032:analysis*; D005456:Fluorescent Dyes / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D008099:Liver / Q000737:chemistry / Q000648:ultrastructure; D051379:Mice; D036641:Microscopy, Fluorescence, Multiphoton / Q000379:methods*; D061848:Optical Imaging / Q000379:methods*; D015027:Zebrafish
chemical_list: D011756:Diphosphates; D005456:Fluorescent Dyes; C107241:diphosphoric acid; D003300:Copper; D004247:DNA
keywords: Fluorescent probe; In vivo imaging; Pyrophosphate; Tissue imaging; Two photon