title: Surface modification and cellular uptake evaluation of Au-coated Ni80Fe20nanodiscs for biomedical applications.
abstract: A nanofabrication technique based on self-assembling of polystyrene nanospheres is used to obtain magnetic Ni80Fe20nanoparticles with a disc shape. The free-standing nanodiscs (NDs) have diameter and thickness of about 630 nm and 30 nm, respectively. The versatility of fabrication technique allows one to cover the ND surface with a protective gold layer with a thickness of about 5 nm. Magnetization reversal has been studied by room-temperature hysteresis loop measurements in water-dispersed free-standing NDs. The reversal shows zero remanence, high susceptibility and nucleation/annihilation fields due to spin vortex formation. In order to investigate their potential use in biomedical applications, the effect of NDs coated with or without the protective gold layer on cell growth has been evaluated. A successful attempt to bind cysteine-fluorescein isothiocyanate (FITC) derivative to the gold surface of magnetic NDs has been exploited to verify the intracellular uptake of the NDs by cytofluorimetric analysis using the FITC conjugate.
authors.affiliation: Nanoscience and Material Division , INRiM , 10135 Torino , Italy.
authors.forename: Gabriele
authors.identifier: 0000-0002-3174-8092
authors.initials: G
authors.lastname: Barrera
authors.affiliation: Department of Drug Science and Technology , University of Turin , 10125 Torino , Italy.
authors.forename: Loredana
authors.initials: L
authors.lastname: Serpe
authors.affiliation: Nanoscience and Material Division , INRiM , 10135 Torino , Italy.
authors.forename: Federica
authors.initials: F
authors.lastname: Celegato
authors.affiliation: Nanoscience and Material Division , INRiM , 10135 Torino , Italy.
authors.forename: Marco
authors.initials: M
authors.lastname: Coїsson
authors.affiliation: Department of Drug Science and Technology , University of Turin , 10125 Torino , Italy.
authors.forename: Katia
authors.initials: K
authors.lastname: Martina
authors.affiliation: Department of Drug Science and Technology , University of Turin , 10125 Torino , Italy.
authors.forename: Roberto
authors.initials: R
authors.lastname: Canaparo
authors.affiliation: Nanoscience and Material Division , INRiM , 10135 Torino , Italy.
authors.forename: Paola
authors.initials: P
authors.lastname: Tiberto
keywords: cytoxicity; magnetic nanodiscs; magnetization reversal; self-assembly