title: Effect of Praeruptorin C on 3-nitropropionic acid induced Huntington's disease-like symptoms in mice.
abstract: Huntington's disease (HD) is an autosomal dominant inherited disease characterized by movement, psychiatric, and cognitive disorders. Previous research suggests that Praeruptorin C (Pra-C), an effective component in the root of Peucedanum praeruptorum dunn, a traditional Chinese medicine, may function in neuroprotection. The present study was conducted to evaluate the effectiveness of Pra-C in the treatment of HD-like symptoms in a 3-nitropropionic acid (3-NP) mouse model, and to explore the possible mechanism of the drug's activity. We treated 3-NP-injected mice with two different doses of Pra-C (1.5 and 3.0mg/kg) for 3 days. Motor behavior was tested using the open field test (OFT) and rotarod test, while psychiatric symptoms were tested using the forced swimming test (FST) and tail suspension test (TST). We found that Pra-C alleviated the motor deficits and depression-like behavior in the 3-NP-treated mice, and protected neurons from excitotoxicity. Western blot analysis revealed that Pra-C upregulated BDNF, DARPP32, and huntingtin protein in the striatum of 3-NP mice. These results taken together suggest that Pra-C may have therapeutic potential with respect to the movement, psychiatric, and cognitive symptoms of HD.
authors.affiliation: Department of Pharmacology, School of Pharmacy, Fourth Military Medical University, 710032 Xi'an, China.
authors.forename: Lu
authors.initials: L
authors.lastname: Wang
authors.affiliation: Training Department of PLA, 710061 Xi'an, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Wang
authors.affiliation: Department of Pharmacology, School of Pharmacy, Fourth Military Medical University, 710032 Xi'an, China.
authors.forename: Le
authors.initials: L
authors.lastname: Yang
authors.affiliation: Department of Pharmacology, School of Pharmacy, Fourth Military Medical University, 710032 Xi'an, China.
authors.forename: Shi-Meng
authors.initials: SM
authors.lastname: Zhou
authors.affiliation: Fourth Military Medical University, 710032 Xi'an, China.
authors.forename: Shao-Yu
authors.initials: SY
authors.lastname: Guan
authors.affiliation: Fourth Military Medical University, 710032 Xi'an, China.
authors.forename: Liu-Kun
authors.initials: LK
authors.lastname: Yang
authors.affiliation: Fourth Military Medical University, 710032 Xi'an, China.
authors.forename: Qi-Xin
authors.initials: QX
authors.lastname: Shi
authors.affiliation: Department of Pharmacology, School of Pharmacy, Fourth Military Medical University, 710032 Xi'an, China.
authors.forename: Ming-Gao
authors.initials: MG
authors.lastname: Zhao
authors.affiliation: Department of Pharmacology, School of Pharmacy, Fourth Military Medical University, 710032 Xi'an, China. Electronic address: yangqifmmu@126.com.
authors.forename: Qi
authors.initials: Q
authors.lastname: Yang
mesh_terms: D000818:Animals; D003374:Coumarins / Q000627:therapeutic use*; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D004365:Drugs, Chinese Herbal / Q000627:therapeutic use*; D006816:Huntington Disease / Q000139:chemically induced* / Q000188:drug therapy* / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D018696:Neuroprotective Agents / Q000627:therapeutic use; D009574:Nitro Compounds / Q000633:toxicity*; D011422:Propionates / Q000633:toxicity*; D016896:Treatment Outcome
chemical_list: D003374:Coumarins; D004365:Drugs, Chinese Herbal; D018696:Neuroprotective Agents; D009574:Nitro Compounds; D011422:Propionates; C058808:praeruptorin C; C015392:3-nitropropionic acid
keywords: 3-NP; Glutamate; Huntington’s disease; Neuroprotection; Striatum