title: Gastrodia elata attenuates inflammatory response by inhibiting the NF-κB pathway in rheumatoid arthritis fibroblast-like synoviocytes.
abstract: Gastrodia elata (GE), which belongs to the Orchidaceae family, was found to possess anti-inflammatory activity. However, the effect of GE on inflammatory response in rheumatoid arthritis fibroblast-like synoviocytes (RA-FLS) remains largely unknown. Thus, the aim of this study was to investigate the effects of GE on tumor necrosis factor-α (TNF-α)-induced inflammatory response in RA-FLS and the underlying molecular mechanism was also explored. Our results demonstrated that GE significantly attenuated TNF-α-induced IL-6 and IL-8 production in RA-FLS. GE also inhibited TNF-α-induced MMP-3 and MMP-13 expression in RA-FLS. Furthermore, pretreatment with GE significantly attenuated TNF-α-induced the expression of p-p65 and IκBα degradation in RA-FLS. In conclusion, this study demonstrated for the first time that GE attenuated inflammatory response by inhibiting the NF-κB pathway signaling in RA-FLS. Thus, GE might have a therapeutic potential towards the treatment of RA.
authors.affiliation: Department of Orthopaedics, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450000, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Li
authors.affiliation: Department of Orthopaedics, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450000, China. Electronic address: wanglm_zz@126.com.
authors.forename: Li-Min
authors.initials: LM
authors.lastname: Wang
authors.affiliation: Department of Orthopaedics, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450000, China.
authors.forename: Jian-Zhong
authors.initials: JZ
authors.lastname: Xu
authors.affiliation: Department of Orthopaedics, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450000, China.
authors.forename: Ke
authors.initials: K
authors.lastname: Tian
authors.affiliation: Department of Orthopaedics, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450000, China.
authors.forename: Chen-Xi
authors.initials: CX
authors.lastname: Gu
authors.affiliation: Department of Orthopaedics, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450000, China.
authors.forename: Zhi-Fu
authors.initials: ZF
authors.lastname: Li
mesh_terms: D000893:Anti-Inflammatory Agents / Q000302:isolation & purification / Q000494:pharmacology*; D018501:Antirheumatic Agents / Q000302:isolation & purification / Q000494:pharmacology*; D001172:Arthritis, Rheumatoid / Q000188:drug therapy* / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D004305:Dose-Response Relationship, Drug; D005347:Fibroblasts / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D031668:Gastrodia / Q000737:chemistry; D006801:Humans; D018836:Inflammation Mediators / Q000378:metabolism*; D015850:Interleukin-6 / Q000378:metabolism; D016209:Interleukin-8 / Q000378:metabolism; D053509:Matrix Metalloproteinase 13 / Q000235:genetics / Q000378:metabolism; D019278:Matrix Metalloproteinase 3 / Q000235:genetics / Q000378:metabolism; D000072000:NF-KappaB Inhibitor alpha / Q000378:metabolism; D016328:NF-kappa B / Q000378:metabolism*; D010766:Phosphorylation; D008517:Phytotherapy; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology*; D010946:Plants, Medicinal; D059748:Proteolysis; D015398:Signal Transduction / Q000187:drug effects; D013583:Synovial Membrane / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D013997:Time Factors; D051996:Transcription Factor RelA / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology
chemical_list: D000893:Anti-Inflammatory Agents; D018501:Antirheumatic Agents; C508606:CXCL8 protein, human; C508600:IL6 protein, human; D018836:Inflammation Mediators; D015850:Interleukin-6; D016209:Interleukin-8; D016328:NF-kappa B; D010936:Plant Extracts; C498143:RELA protein, human; D051996:Transcription Factor RelA; D014409:Tumor Necrosis Factor-alpha; D000072000:NF-KappaB Inhibitor alpha; C507079:MMP13 protein, human; D053509:Matrix Metalloproteinase 13; C511768:MMP3 protein, human; D019278:Matrix Metalloproteinase 3
keywords: Gastrodia elata; Inflammation; Rheumatoid arthritis fibroblast-like synoviocytes; Tumor necrosis factor-α