title: Protective effect ofMelissa officinalisextract against H2O2-induced oxidative stress in human vascular endothelial cells.
abstract: Melissa officinalisL. is a medicinal plant with a large variety of pharmacological effects and traditional applications. This study aimed to evaluate the protective and antioxidant activities of the extract ofM. officinalisaerial parts on human umbilical vein endothelial cells (HUVECs) under oxidative stress induced by H2O2. Cells were incubated with H2O2(0.5 mM, 2 h) after pretreatment withM. officinalisextract (25-500 μg/mL). Cell viability was evaluated by 3-(4, 5- Dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay. The concentration of hydroperoxides and ferric reducing antioxidant power (FRAP) were measured in intra- and extra-cellular fluids. Pretreatment of HUVECs withM. officinalisextract at the concentrations of 100-500 μg/mL improved the cell viability after exposure to H2O2significantly. It also decreased hydroperoxides concentration and increased FRAP value in both intra- and extra-cellular fluids. The results revealed antioxidant and cytoprotective effects ofM. officinalisagainst H2O2-induced oxidative stress in HUVECs. Due to the valuable antioxidant activity, this plant extract may have potential benefits for the prevention of cardiovascular diseases associated with oxidative stress.
authors.affiliation: Department of Pharmacology & Toxicology and Isfahan Pharmaceutical Sciences Research Center, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, I.R. Iran.
authors.forename: Leila
authors.initials: L
authors.lastname: Safaeian
authors.affiliation: Department of Pharmacognosy and Isfahan Pharmaceutical Sciences Research Center, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, I.R. Iran.
authors.forename: Seyyed Ebrahim
authors.initials: SE
authors.lastname: Sajjadi
authors.affiliation: Applied Physiology Research Center, Cardiovascular Research Institute, Department of Physiology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, I.R. Iran.
authors.forename: Shaghayegh Haghjooy
authors.initials: SH
authors.lastname: Javanmard
authors.affiliation: Department of Pharmacology & Toxicology and Isfahan Pharmaceutical Sciences Research Center, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, I.R. Iran.
authors.forename: Hossein
authors.initials: H
authors.lastname: Montazeri
authors.affiliation: Applied Physiology Research Center, Cardiovascular Research Institute, Department of Physiology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, I.R. Iran.
authors.forename: Fariba
authors.initials: F
authors.lastname: Samani
keywords: Antioxidant; HUVECs; Melissa officinalis L.; Oxidative stress