title: Na+/HCO3-cotransporter is expressed on β and α cells during rat pancreatic development.
abstract: OBJECTIVE
  To determine the expression and localization of the electrogenic Na+/HCO3-cotransporter (NBC1) in rat pancreas during development.
  
  
  METHODS
  The rat pancreas from postnatal and embryos removed from the uterus of pregnant rats that had been sacrificed by CO2asphyxiation were used. Rat pancreas from embryonic day (E) 15.5 and E18.5 rat embryos was isolated under a stereomicroscope. Rat pancreas from postnatal (P) days 0, 7, 14, 21 and adult was directly isolated by the unaided eye. The RT-PCR analysis of the NBC1 specific region on rat pancreas tissues from different developmental stages. The two antibodies which target the NBC1 common COOH-terminal region and NH2-terminal region detected a clear band of about 145 kDa in the Western blot analysis. The localization of NBC1 was examined by immuno-fluorescence detection.
  
  
  RESULTS
  The results revealed the first peak of NBC1 expression at E18.5 and the second peak at P14. Meanwhile, the low NBC1 expression occurred at P7 and adult stages. Our results demonstrated, for the first time, the presence of NBC1 in the plasma membrane of β and α cells, as well as in the basolateral membrane of acinar cells of the rat pancreas at different stages of development.
  
  
  CONCLUSIONS
  The data strongly suggests that NBC1 is diversely expressed in the pancreas at different developmental stages, where it may exert its functions in pancreatic development especially islet cell growth through HCO3-transport and pH regulation.
authors.affiliation: Li-Hua Cao, Ning Wang, Wei De, Department of Biochemistry and Molecular Biology, Nanjing Medical University, Nanjing 210029, Jiangsu Province, China.
authors.forename: Li-Hua
authors.initials: LH
authors.lastname: Cao
authors.affiliation: Li-Hua Cao, Ning Wang, Wei De, Department of Biochemistry and Molecular Biology, Nanjing Medical University, Nanjing 210029, Jiangsu Province, China.
authors.forename: Cheng-Cai
authors.initials: CC
authors.lastname: Xia
authors.affiliation: Li-Hua Cao, Ning Wang, Wei De, Department of Biochemistry and Molecular Biology, Nanjing Medical University, Nanjing 210029, Jiangsu Province, China.
authors.forename: Zhao-Chun
authors.initials: ZC
authors.lastname: Shi
authors.affiliation: Li-Hua Cao, Ning Wang, Wei De, Department of Biochemistry and Molecular Biology, Nanjing Medical University, Nanjing 210029, Jiangsu Province, China.
authors.forename: Ning
authors.initials: N
authors.lastname: Wang
authors.affiliation: Li-Hua Cao, Ning Wang, Wei De, Department of Biochemistry and Molecular Biology, Nanjing Medical University, Nanjing 210029, Jiangsu Province, China.
authors.forename: Zheng-Hua
authors.initials: ZH
authors.lastname: Gu
authors.affiliation: Li-Hua Cao, Ning Wang, Wei De, Department of Biochemistry and Molecular Biology, Nanjing Medical University, Nanjing 210029, Jiangsu Province, China.
authors.forename: Li-Zhi
authors.initials: LZ
authors.lastname: Yu
authors.affiliation: Li-Hua Cao, Ning Wang, Wei De, Department of Biochemistry and Molecular Biology, Nanjing Medical University, Nanjing 210029, Jiangsu Province, China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Wan
authors.affiliation: Li-Hua Cao, Ning Wang, Wei De, Department of Biochemistry and Molecular Biology, Nanjing Medical University, Nanjing 210029, Jiangsu Province, China.
authors.forename: Wei
authors.initials: W
authors.lastname: De
mesh_terms: D000367:Age Factors; D000818:Animals; D000831:Animals, Newborn; D015153:Blotting, Western; D005455:Fluorescent Antibody Technique; D018507:Gene Expression Regulation, Developmental; D005865:Gestational Age; D050416:Glucagon-Secreting Cells / Q000378:metabolism*; D050417:Insulin-Secreting Cells / Q000378:metabolism*; D009024:Morphogenesis; D010179:Pancreas / Q000196:embryology / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D017207:Rats, Sprague-Dawley; D020133:Reverse Transcriptase Polymerase Chain Reaction; D027982:Sodium-Bicarbonate Symporters / Q000235:genetics / Q000378:metabolism*
chemical_list: D012333:RNA, Messenger; C422004:Slc4a4 protein, rat; D027982:Sodium-Bicarbonate Symporters
keywords: Immunolocalization; Na+/HCO3- cotransporter; Pancreatic development; α cells; β cells