title: Antibacterial and anticancer PDMS surface for mammalian cell growth using the Chinese herb extract paeonol(4-methoxy-2-hydroxyacetophenone).
abstract: Polydimethylsiloxane (PDMS) is widely used as a cell culture platform to produce micro- and nano-technology based microdevices. However, the native PDMS surface is not suitable for cell adhesion and is always subject to bacterial pollution and cancer cell invasion. Coating the PDMS surface with antibacterial or anticancer materials often causes considerable harm to the non-cancer mammalian cells on it. We have developed a method to fabricate a biocompatible PDMS surface which not only promotes non-cancer mammalian cell growth but also has antibacterial and anticancer activities, by coating the PDMS surface with a Chinese herb extract, paeonol. Coating changes the wettability and the elemental composition of the PDMS surface. Molecular dynamic simulation indicates that the absorption of paeonol onto the PDMS surface is an energy favourable process. The paeonol-coated PDMS surface exhibits good antibacterial activity against both Gram-positive and Gram-negative bacteria. Moreover considerable antibacterial activity is maintained after the coated surface is rinsed or incubated in water. The coated PDMS surface inhibits bacterial growth on the contact surface and promotes non-cancer mammalian cell growth with low cell toxicity; meanwhile the growth of cancer cells is significantly inhibited. Our study will potentially guide PDMS surface modification approaches to produce biomedical devices.
authors.affiliation: State Key Laboratory of Medicinal Chemical Biology, The Key Laboratory of Bioactive Materials, Ministry of Education, College of Life Science, Nankai University, Tianjin, 300071, China.
authors.forename: Jiajia
authors.initials: J
authors.lastname: Jiao
authors.affiliation: Institute of Physical Chemistry Polish Academy of Sciences, Kasprzaka 44/52, 01-224 Warsaw, Poland.
authors.forename: Lili
authors.initials: L
authors.lastname: Sun
authors.affiliation: TEDA Hospital, No. 65 Third Avenue, Economic-Technological Development Area, Tianjin, 300457, China.
authors.forename: Zaiyu
authors.initials: Z
authors.lastname: Guo
authors.affiliation: Institute of Physical Chemistry Polish Academy of Sciences, Kasprzaka 44/52, 01-224 Warsaw, Poland.
authors.forename: Sen
authors.initials: S
authors.lastname: Hou
authors.affiliation: Institute of Physical Chemistry Polish Academy of Sciences, Kasprzaka 44/52, 01-224 Warsaw, Poland.
authors.forename: Robert
authors.initials: R
authors.lastname: Holyst
authors.affiliation: TEDA Hospital, No. 65 Third Avenue, Economic-Technological Development Area, Tianjin, 300457, China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Lu
authors.affiliation: State Key Laboratory of Medicinal Chemical Biology, The Key Laboratory of Bioactive Materials, Ministry of Education, College of Life Science, Nankai University, Tianjin, 300071, China.
authors.forename: Xizeng
authors.initials: X
authors.lastname: Feng
mesh_terms: D000098:Acetophenones / Q000737:chemistry / Q000494:pharmacology*; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D000970:Antineoplastic Agents / Q000494:pharmacology*; D049108:Cell Enlargement; D020099:Coated Materials, Biocompatible / Q000737:chemistry / Q000494:pharmacology*; D004129:Dimethylpolysiloxanes / Q000737:chemistry / Q000494:pharmacology*; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000494:pharmacology; D004926:Escherichia coli / Q000187:drug effects; D006367:HeLa Cells / Q000187:drug effects; D006801:Humans; D008422:Materials Testing; D051379:Mice; D008958:Models, Molecular; D056004:Molecular Dynamics Simulation; D013499:Surface Properties
chemical_list: D000098:Acetophenones; D000900:Anti-Bacterial Agents; D000970:Antineoplastic Agents; D020099:Coated Materials, Biocompatible; D004129:Dimethylpolysiloxanes; D004365:Drugs, Chinese Herbal; C013638:paeonol; C013830:baysilon