title: Subchronic perfluorooctanesulfonate (PFOS) exposure induces elevated mutant frequency in an in vivo λ transgenic medaka mutation assay.
abstract: Perfluorooctanesulfonate (PFOS) has been widely detected in the environment, wildlife and humans, but few studies have ever examined its mutagenic effect in vivo. In the present study, we use a transgenic fish model, the λ transgenic medaka, to evaluate the potential mutagenicity of PFOS in vivo following a subchronic exposure of 30 days. The mutant frequency of cII target gene was 3.46 × 10-5in liver tissue from control fish, which increased by 1.4-fold to 4.86 × 10-5in fish exposed to 6.7 μg/L PFOS, 1.55-fold to 5.36 × 10-5in fish exposed to 27.6 μg/L PFOS, and 2.02-fold to 6.99 × 10-5in fish exposed to 87.6 μg/L PFOS. This dose-dependent increase of mutant frequency was also accompanied with mutational spectrum changes associated with PFOS exposure. In particular, PFOS-induced mutation was characterized by +1 frameshift mutations, which increased from 0% in control fish to 13.2% in fish exposed to 27.6 μg/L PFOS and 14.6% in fish exposed to 87.6 μg/L PFOS. Our findings provide the first evidence of PFOS's mutagenicity in an aquatic model system. Given the fact that most conventional mutagenic assays were negative for PFOS, we propose that PFOS-induced mutation in liver tissue of λ transgenic medaka may be mediated through compromised liver function.
authors.affiliation: Institute of Environmental Safety and Human Health, Wenzhou Medical University, Wenzhou 325035, P.R. China.
authors.forename: Yuanhong
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Institute of Environmental Safety and Human Health, Wenzhou Medical University, Wenzhou 325035, P.R. China.
authors.forename: Wei
authors.initials: W
authors.lastname: Hu
authors.affiliation: Institute of Environmental Safety and Human Health, Wenzhou Medical University, Wenzhou 325035, P.R. China.
authors.forename: Changjiang
authors.initials: C
authors.lastname: Huang
authors.affiliation: Institute of Environmental Safety and Human Health, Wenzhou Medical University, Wenzhou 325035, P.R. China.
authors.forename: Shushan
authors.initials: S
authors.lastname: Hua
authors.affiliation: Institute of Environmental Safety and Human Health, Wenzhou Medical University, Wenzhou 325035, P.R. China.
authors.forename: Qihao
authors.initials: Q
authors.lastname: Wei
authors.affiliation: Institute of Environmental Safety and Human Health, Wenzhou Medical University, Wenzhou 325035, P.R. China.
authors.forename: Chenglian
authors.initials: C
authors.lastname: Bai
authors.affiliation: Institute of Environmental Safety and Human Health, Wenzhou Medical University, Wenzhou 325035, P.R. China.
authors.forename: Jiangfei
authors.initials: J
authors.lastname: Chen
authors.affiliation: Aquatic Biotechnology and Environmental Laboratory, Warnell School of Forest Resources, University of Georgia, Athens, Georgia, USA.
authors.forename: Michelle B
authors.initials: MB
authors.lastname: Norris
authors.affiliation: Aquatic Biotechnology and Environmental Laboratory, Warnell School of Forest Resources, University of Georgia, Athens, Georgia, USA.
authors.forename: Richard
authors.initials: R
authors.lastname: Winn
authors.affiliation: Institute of Environmental Safety and Human Health, Wenzhou Medical University, Wenzhou 325035, P.R. China.
authors.forename: Dongren
authors.initials: D
authors.lastname: Yang
authors.affiliation: Institute of Environmental Safety and Human Health, Wenzhou Medical University, Wenzhou 325035, P.R. China.
authors.forename: Qiaoxiang
authors.initials: Q
authors.lastname: Dong
mesh_terms: D017738:Alkanesulfonic Acids / Q000633:toxicity*; D000818:Animals; D030801:Animals, Genetically Modified / Q000235:genetics; D002273:Carcinogens / Q000633:toxicity*; D005466:Fluorocarbons / Q000633:toxicity*; D008099:Liver / Q000187:drug effects* / Q000473:pathology; D009152:Mutagenicity Tests; D009154:Mutation / Q000187:drug effects* / Q000235:genetics; D009990:Oryzias / Q000235:genetics
chemical_list: D017738:Alkanesulfonic Acids; D002273:Carcinogens; D005466:Fluorocarbons; C076994:perfluorooctane sulfonic acid