title: Identification of non-coding and coding RNAs in porcine endometrium.
abstract: One of the most critical periods of embryonic loss in pig is day 12 of pregnancy, when implantation begins. Here, we analyzed the gene expression on day 12 of pregnancy and non-pregnancy in the porcine endometrium using RNA sequencing (RNA-seq). 237 mRNAs, 34 lncRNAs and 1 miRNA were significantly differentially expressed between the two groups. Further functional analyses were conducted to identify these differentially expressed transcripts. The results demonstrated that they participate in various biological processes, such as cell adhesion, binding, nucleic and metabolic processes. In addition, our results showed that the differentially expressed genes (IL1R, FGF9, DUPS10, DUPS4, CD14 and MAP4K4) in MAPK pathway, and lncRNAs of XLOC_2604764 and XLOC_2604756 may play a vital role in regulating embryo implantation. Besides, we investigated the lncRNA-ssc-miR-132-mRNA interactions, aiming to explain the regulatory networks of coding and non-coding genes that contributes to the establishment of the maternal pregnancy.
authors.affiliation: Key Lab of Swine Genetics and Breeding of Ministry of Agriculture, Key Lab of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan, Hubei, PR China.
authors.forename: Yueying
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Key Lab of Swine Genetics and Breeding of Ministry of Agriculture, Key Lab of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan, Hubei, PR China.
authors.forename: Tao
authors.initials: T
authors.lastname: Hu
authors.affiliation: Key Lab of Swine Genetics and Breeding of Ministry of Agriculture, Key Lab of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan, Hubei, PR China.
authors.forename: Lihang
authors.initials: L
authors.lastname: Wu
authors.affiliation: Key Lab of Swine Genetics and Breeding of Ministry of Agriculture, Key Lab of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan, Hubei, PR China.
authors.forename: Xiaoran
authors.initials: X
authors.lastname: Liu
authors.affiliation: Key Lab of Swine Genetics and Breeding of Ministry of Agriculture, Key Lab of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan, Hubei, PR China.
authors.forename: Songyi
authors.initials: S
authors.lastname: Xue
authors.affiliation: Key Lab of Swine Genetics and Breeding of Ministry of Agriculture, Key Lab of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan, Hubei, PR China. Electronic address: leimg@mail.hzau.edu.cn.
authors.forename: Minggang
authors.initials: M
authors.lastname: Lei
mesh_terms: D000818:Animals; D010064:Embryo Implantation; D004717:Endometrium / Q000378:metabolism*; D005260:Female; D035683:MicroRNAs / Q000235:genetics*; D011247:Pregnancy; D062085:RNA, Long Noncoding / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics*; D017423:Sequence Analysis, RNA; D015398:Signal Transduction; D034421:Sus scrofa / Q000235:genetics / Q000378:metabolism*; D059467:Transcriptome
chemical_list: D035683:MicroRNAs; D062085:RNA, Long Noncoding; D012333:RNA, Messenger
keywords: Coding RNA; Embryo implantation; Endometrium; Non-coding RNA; Pig