title: Evaluation of the antibacterial activity of a cationic polymer in aqueous solution with a convenient electrochemical method.
abstract: Quaternized chitosan is a cationic biopolymer with good antibacterial activity, biocompatibility, and biodegradability, and it has been widely applied in many fields. We have developed a convenient method to evaluate the antibacterial activity of hydroxypropyltrimethylammonium chloride chitosan (HACC) with a nonionic surfactant poloxamer in aqueous solution by monitoring the change of the oxidation peak current in cyclic voltammetry. Increasing values of the oxidation peak current were positively correlated with the antibacterial activity of HACC-poloxamer solutions. Optical microscope images, the zeta potential, and fluorescence spectroscopy showed that the aggregation state of HACC-poloxamer was related to the ratio of the two polymers and also to the antibacterial activity and oxidation peak current. At an HACC-to-poloxamer ratio of 1:0.75, the maximum surface charge density and the smooth edge of HACC-poloxamer aggregates can accelerate diffusion in aqueous solution. It is expected that this convenient method can be applied for a quick evaluation of the antibacterial activity of cationic biopolymers in aqueous solution. Graphical Abstract The cyclic voltammograms of MB in HACC/poloxamer solution, and the antibacterial efficiency against S. aureus after incubated with HACC (a) and 1/0.75 of HACC/poloxamer (b).
authors.affiliation: School of Chemistry and Chemical Engineering, Southeast University, Nanjing, Jiangsu, 211189, China. yihongwang@seu.edu.cn.
authors.forename: Yihong
authors.initials: Y
authors.lastname: Wang
authors.affiliation: School of Chemistry and Chemical Engineering, Southeast University, Nanjing, Jiangsu, 211189, China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Guo
authors.affiliation: School of Chemistry and Chemical Engineering, Southeast University, Nanjing, Jiangsu, 211189, China.
authors.forename: Huafu
authors.initials: H
authors.lastname: Wang
authors.affiliation: School of Chemistry and Chemical Engineering, Southeast University, Nanjing, Jiangsu, 211189, China.
authors.forename: Kun
authors.initials: K
authors.lastname: Qian
authors.affiliation: School of Chemistry and Chemical Engineering, Southeast University, Nanjing, Jiangsu, 211189, China.
authors.forename: Liang
authors.initials: L
authors.lastname: Tian
authors.affiliation: School of Chemistry and Chemical Engineering, Southeast University, Nanjing, Jiangsu, 211189, China.
authors.forename: Chen
authors.initials: C
authors.lastname: Yao
authors.affiliation: School of Chemistry and Chemical Engineering, Southeast University, Nanjing, Jiangsu, 211189, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Song
authors.affiliation: Hydron Contact Lens Co. Ltd., Danyang, Jiangsu, 212331, China.
authors.forename: Weixia
authors.initials: W
authors.lastname: Shu
authors.affiliation: Hydron Contact Lens Co. Ltd., Danyang, Jiangsu, 212331, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Chen
authors.affiliation: School of Chemistry and Chemical Engineering, Southeast University, Nanjing, Jiangsu, 211189, China.
authors.forename: Jinxu
authors.initials: J
authors.lastname: Qi
mesh_terms: D064751:Ammonium Compounds / Q000737:chemistry / Q000494:pharmacology; D000900:Anti-Bacterial Agents / Q000737:chemistry* / Q000494:pharmacology*; D048271:Chitosan / Q000031:analogs & derivatives* / Q000494:pharmacology*; D055664:Electrochemical Techniques / Q000379:methods; D006801:Humans; D008826:Microbial Sensitivity Tests / Q000379:methods; D020442:Poloxamer / Q000737:chemistry* / Q000494:pharmacology*; D013203:Staphylococcal Infections / Q000188:drug therapy; D013211:Staphylococcus aureus / Q000187:drug effects*; D013501:Surface-Active Agents / Q000737:chemistry / Q000494:pharmacology
chemical_list: D064751:Ammonium Compounds; D000900:Anti-Bacterial Agents; D013501:Surface-Active Agents; D020442:Poloxamer; D048271:Chitosan
keywords: Antibacterial activity; Cyclic voltammetry; Hydroxypropyltrimethylammonium chloride chitosan–poloxamer; Quaternized chitosan