title: Active immunization with Brucella abortus S19 phage lysate elicits serum IgG that protects guinea pigs against virulent B. abortus and protects mice by passive immunization.
abstract: Brucellosis is an economically important zoonosis of worldwide significance. Earlier (Jain et al., 2015) we reported methodology for generation of phage lysate preparations against Brucella abortus S19 using brucellaphage 'ϕLd'. In this study, using a fixed dose (Two mouse PD100) of lysates, the prophylactic efficacies of both plain and alum gel adjuvanted lysates were evaluated in guinea pig by direct virulent challenge and passive mouse protection test (PMPT). Strong humoral and cell mediated immune responses in guinea pigs and protection comparable to S19 vaccine was observed with low dose (1.0 μg protein and 120 μg carbohydrate adsorbed on 0.1% aluminium gel). Passive transfer of antibodies to mice using d 90 post immunization sera of guinea pig protected the animals against challenge. The study suggested the significance of humoral immunity in murine brucellosis. Further, the methodology can be explored to produce a new class of immunotherapeutic agents against bovine brucellosis.
authors.affiliation: Division of Biological Standardization, Indian Veterinary Research Institute, Izatnagar, UP, 243122, India. Electronic address: jain.lata59@gmail.com.
authors.forename: Lata
authors.initials: L
authors.lastname: Jain
authors.affiliation: Division of Biological Standardization, Indian Veterinary Research Institute, Izatnagar, UP, 243122, India.
authors.forename: Mayank
authors.initials: M
authors.lastname: Rawat
authors.affiliation: Immunology Section, Indian Veterinary Research Institute, Izatnagar, UP, 243122, India.
authors.forename: Saravanan
authors.initials: S
authors.lastname: Ramakrishnan
authors.affiliation: Division of Biological Products, Indian Veterinary Research Institute, Izatnagar, UP, 243122, India.
authors.forename: Bablu
authors.initials: B
authors.lastname: Kumar
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000276:immunology / Q000494:pharmacology; D001435:Bacteriophages; D002003:Brucella abortus / Q000737:chemistry / Q000276:immunology / Q000821:virology; D002006:Brucellosis / Q000276:immunology / Q000628:therapy*; D002417:Cattle; D006168:Guinea Pigs; D007116:Immunization, Passive; D051379:Mice
chemical_list: D000907:Antibodies, Bacterial
keywords: Brucellosis; Cell mediate immune response; Humoral response; Passive mouse protection assay; Phage lysate; Protective efficacy