title: Affibody-displaying bio-nanocapsules effective in EGFR, typical biomarker, expressed in various cancer cells.
abstract: The expression of epidermal growth factor receptor (EGFR) across a wide range of tumor cells has attracted attention for use as a tumor marker in drug delivery systems. Therefore, binding molecules with the ability to target EGFR have been developed. Among them, we focused on affibodies that are binding proteins derived from staphylococcal protein A. By displaying affibody (ZEGFR) binding to EGFR on the surface of a bio-nanocapsule (BNC) derived from a hepatitis B virus (HBV), we developed an altered BNC (ZEGFR-BNC) with a high specificity to EGFR-expressing cells. We considered two different types of ZEGFR(Z955 and Z1907), and found that the Z1907 dimer-displaying BNC ([Z1907]2-BNC) could effectively bind to EGFR-expressing cells and deliver drugs to the cytosol. Since this study showed that [Z1907]2-BNC could target EGFR-expressing cells, we would use this particle as a drug delivery carrier for various cancer cells expressing EGFR.
authors.affiliation: Graduate School of Science, Technology and Innovation, Kobe University, Japan. Electronic address: nyuya@landscape.kobe-u.ac.jp.
authors.forename: Yuya
authors.initials: Y
authors.lastname: Nishimura
authors.affiliation: Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, Japan. Electronic address: 134t410t@stu.kobe-u.ac.jp.
authors.forename: Ryosuke
authors.initials: R
authors.lastname: Ezawa
authors.affiliation: Graduate School of Science, Technology and Innovation, Kobe University, Japan. Electronic address: junjun@port.kobe-u.ac.jp.
authors.forename: Jun
authors.initials: J
authors.lastname: Ishii
authors.affiliation: Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, Japan. Electronic address: ochiaki@port.kobe-u.ac.jp.
authors.forename: Chiaki
authors.initials: C
authors.lastname: Ogino
authors.affiliation: Graduate School of Science, Technology and Innovation, Kobe University, Japan; Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, Japan. Electronic address: akondo@kobe-u.ac.jp.
authors.forename: Akihiko
authors.initials: A
authors.lastname: Kondo
mesh_terms: D015415:Biomarkers / Q000737:chemistry; D045744:Cell Line, Tumor; D004305:Dose-Response Relationship, Drug; D004337:Drug Carriers / Q000737:chemistry; D016503:Drug Delivery Systems; D066246:ErbB Receptors / Q000037:antagonists & inhibitors* / Q000096:biosynthesis; D006515:Hepatitis B virus / Q000737:chemistry; D006801:Humans; D015394:Molecular Structure; D053769:Nanocapsules / Q000737:chemistry*; D013329:Structure-Activity Relationship
chemical_list: D015415:Biomarkers; D004337:Drug Carriers; D053769:Nanocapsules; D066246:ErbB Receptors
keywords: Affibody; Anticancer; Bio-nanocapsule; Drug delivery; Epidermal growth factor receptor