title: Cannabidiol Activates Neuronal Precursor Genes in Human Gingival Mesenchymal Stromal Cells.
abstract: In the last years, mesenchymal stromal cells (MSCs) from oral tissues have received considerable interest in regenerative medicine since they can be obtained with minimal invasive procedure and exhibit immunomodulatory properties. This study was aimed to investigate whether in vitro pre-treatment of MSCs obtained from human gingiva (hGMSCs) with Cannabidiol (CBD), a cannabinoid component produced by the plant Cannabis sativa, may promote human gingiva derived MSCs to differentiate toward neuronal precursor cells. Specifically, we have treated the hGMSCs with CBD (5 µM) for 24 h in order to evaluate the expression of genes involved in cannabidiol signaling, cell proliferation, self-renewal and multipotency, and neural progenitor cells differentiation. Next generation sequencing (NGS) demonstrated that CBD activates genes associated with G protein coupled receptor signaling in hGMSCs. Genes involved in DNA replication, cell cycle, proliferation, and apoptosis were regulated. Moreover, genes associated with the biological process of neuronal progenitor cells (NCPs) proliferation, neuron differentiation, neurogenesis, and nervous system development were significantly modulated. From our results, we hypothesize that human gingiva-derived MSCs conditioned with CBD could represent a valid method for improving the hGMSCs phenotype and thus might be a potential therapeutic tool in the treatment of neurodegenerative diseases. J. Cell. Biochem. 118: 1531-1546, 2017. © 2016 Wiley Periodicals, Inc.
authors.affiliation: IRCCS Centro Neurolesi "Bonino-Pulejo", Messina 98124, Italy.
authors.forename: Thangavelu
authors.initials: T
authors.lastname: Soundara Rajan
authors.affiliation: IRCCS Centro Neurolesi "Bonino-Pulejo", Messina 98124, Italy.
authors.forename: Sabrina
authors.initials: S
authors.lastname: Giacoppo
authors.affiliation: IRCCS Centro Neurolesi "Bonino-Pulejo", Messina 98124, Italy.
authors.forename: Domenico
authors.initials: D
authors.lastname: Scionti
authors.affiliation: Stem Cells and Regenerative Medicine Laboratory, Department of Medical, Oral and Biotechnological Sciences, University "G. d'Annunzio", Chieti 66100, Italy.
authors.forename: Francesca
authors.initials: F
authors.lastname: Diomede
authors.affiliation: Council for Research and Experimentation in Agriculture-Research Centre for Industrial Crops (CRA-CIN), Rovigo, Italy.
authors.forename: Gianpaolo
authors.initials: G
authors.lastname: Grassi
authors.affiliation: Dipartimento di Scienze del Farmaco, Università del Piemonte Orientale, Novara 28100, Italy.
authors.forename: Federica
authors.initials: F
authors.lastname: Pollastro
authors.affiliation: Stem Cells and Regenerative Medicine Laboratory, Department of Medical, Oral and Biotechnological Sciences, University "G. d'Annunzio", Chieti 66100, Italy.
authors.forename: Adriano
authors.initials: A
authors.lastname: Piattelli
authors.affiliation: IRCCS Centro Neurolesi "Bonino-Pulejo", Messina 98124, Italy.
authors.forename: Placido
authors.initials: P
authors.lastname: Bramanti
authors.affiliation: IRCCS Centro Neurolesi "Bonino-Pulejo", Messina 98124, Italy.
authors.forename: Emanuela
authors.initials: E
authors.lastname: Mazzon
authors.affiliation: Stem Cells and Regenerative Medicine Laboratory, Department of Medical, Oral and Biotechnological Sciences, University "G. d'Annunzio", Chieti 66100, Italy.
authors.forename: Oriana
authors.initials: O
authors.lastname: Trubiani
mesh_terms: D002185:Cannabidiol / Q000494:pharmacology*; D002454:Cell Differentiation / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D005786:Gene Expression Regulation / Q000187:drug effects; D005881:Gingiva / Q000166:cytology* / Q000187:drug effects; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods*; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000187:drug effects; D055495:Neurogenesis / Q000187:drug effects; D009474:Neurons / Q000166:cytology*; D017423:Sequence Analysis, RNA / Q000379:methods*; D015398:Signal Transduction
chemical_list: D002185:Cannabidiol
keywords: CANNABIDIOL; CELL PROLIFERATION; HUMAN GINGIVAL MESENCHYMAL STROMAL CELLS; NEURAL PROGENITOR CELLS; REGENERATIVE MEDICINE