title: Apoptotic effect of chalcone derivatives of 2-acetylthiophene in human breast cancer cells.
abstract: BACKGROUND
  A variety of chalcones have demonstrated cytotoxic activity toward several cancer cell lines. This study aimed to investigate the cytotoxicity of four chalcones derivatives of 2-acetylthiophene in human breast cancer cell lines.
  
  
  METHODS
  MCF-7 and MDA-MB-231 cells were treated with synthesized chalcones and the cytotoxicity was evaluated by tetrazolium dye (MTT), live/dead, and DAPI assays.
  
  
  RESULTS
  Chalcones significantly decreased MCF-7 and MDA-MB-231 cells viability in vitro in a dose dependent manner. After 48h treatment, the IC50values ranging from 5.52 to 34.23μM. Chalcone 3c displayed the highest cytotoxic activity from all the tested compounds. Cytotoxic effects of compounds were confirmed in the live/dead assay. In addition, DAPI staining revealed that these compounds induce death by apoptosis.
  
  
  CONCLUSIONS
  The data speculate that chalcone derivatives of 2-acetylthiophene may represent a source of therapeutic agents for human breast cancer.
authors.affiliation: Cancer Biotechnology Laboratory, Research Group on Cellular and Molecular Oncology, Technology Development Center, Federal University of Pelotas, Pelotas, RS, Brazil.
authors.forename: Tatiana B
authors.initials: TB
authors.lastname: Fogaça
authors.affiliation: Cancer Biotechnology Laboratory, Research Group on Cellular and Molecular Oncology, Technology Development Center, Federal University of Pelotas, Pelotas, RS, Brazil.
authors.forename: Rosiane M
authors.initials: RM
authors.lastname: Martins
authors.affiliation: Cancer Biotechnology Laboratory, Research Group on Cellular and Molecular Oncology, Technology Development Center, Federal University of Pelotas, Pelotas, RS, Brazil.
authors.forename: Karine R
authors.initials: KR
authors.lastname: Begnini
authors.affiliation: Center of Chemical, Pharmaceutical and Food Sciences, Federal University of Pelotas, RS, Brazil.
authors.forename: Caroline
authors.initials: C
authors.lastname: Carapina
authors.affiliation: Center of Chemical, Pharmaceutical and Food Sciences, Federal University of Pelotas, RS, Brazil.
authors.forename: Marina
authors.initials: M
authors.lastname: Ritter
authors.affiliation: Center of Chemical, Pharmaceutical and Food Sciences, Federal University of Pelotas, RS, Brazil.
authors.forename: Claudio M P
authors.initials: CM
authors.lastname: de Pereira
authors.affiliation: Cancer Biotechnology Laboratory, Research Group on Cellular and Molecular Oncology, Technology Development Center, Federal University of Pelotas, Pelotas, RS, Brazil.
authors.forename: Fabiana K
authors.initials: FK
authors.lastname: Seixas
authors.affiliation: Cancer Biotechnology Laboratory, Research Group on Cellular and Molecular Oncology, Technology Development Center, Federal University of Pelotas, Pelotas, RS, Brazil. Electronic address: collares.t@gmail.com.
authors.forename: Tiago
authors.initials: T
authors.lastname: Collares
mesh_terms: D017209:Apoptosis / Q000187:drug effects* / Q000502:physiology; D001943:Breast Neoplasms / Q000188:drug therapy / Q000473:pathology; D002470:Cell Survival / Q000187:drug effects / Q000502:physiology; D002599:Chalcone / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use; D004305:Dose-Response Relationship, Drug; D005260:Female; D006131:Growth Inhibitors / Q000494:pharmacology; D006801:Humans; D061986:MCF-7 Cells; D013876:Thiophenes / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use
chemical_list: D006131:Growth Inhibitors; D013876:Thiophenes; C508419:2-acetylthiophene; D002599:Chalcone
keywords: Anticancer; Apoptosis; Chalcone; Cytotoxicity