title: Analysis of four toxic metals in a single rice seed by matrix solid phase dispersion -inductively coupled plasma mass spectrometry.
abstract: Toxic metals in rice pose great risks to human health. Metal bioaccumulation in rice grains is a criterion of breeding. Rice breeding requires a sensitive method to determine metal content in single rice grains to assist the variety selection. In the present study, four toxic metals of arsenic (As), cadmium (Cd), chromium (Cr) and lead (Pb) in a single rice grain were determined by a simple and rapid method. The developed method is based on matrix solid phase dispersion using multi-wall carbon nanotubes (MWCNTs) as dispersing agent and analyzed by inductively coupled plasma mass spectrometry. The experimental parameters were systematically investigated. The limits of detection (LOD) were 5.0, 0.6, 10 and 2.1 ng g-1for As, Cd, Cr, and Pb, respectively, with relative standard deviations (n = 6) of <7.7%, demonstrating the good sensitivity and precision of the method. The results of 30 real world rice samples analyzed by this method agreed well with those obtained by the standard microwave digestion. The amount of sample required was reduced approximately 100 fold in comparison with the microwave digestion. The method has a high application potential for other sample matrices and elements with high sensitivity and sample throughput.
authors.affiliation: Analysis &Testing Center, Chinese Academy of Tropical Agricultural Sciences, Hainan Provincial Key Laboratory of Quality and Safety for Tropical Fruits and Vegetables, Haikou, Hainan 571101, China.
authors.forename: Xiufen
authors.initials: X
authors.lastname: He
authors.affiliation: Mathematical Department, Qiongtai Teachers College, Haikou, Hainan 571100, China.
authors.forename: Lixia
authors.initials: L
authors.lastname: Chen
authors.affiliation: Environment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou, Hainan 571101, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Chen
authors.affiliation: College of Environment and Plant Protection, Hainan University, Haikou, Hainan 570228, China.
authors.forename: Huamei
authors.initials: H
authors.lastname: Yu
authors.affiliation: Environment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou, Hainan 571101, China.
authors.forename: Lixu
authors.initials: L
authors.lastname: Peng
authors.affiliation: Analysis &Testing Center, Chinese Academy of Tropical Agricultural Sciences, Hainan Provincial Key Laboratory of Quality and Safety for Tropical Fruits and Vegetables, Haikou, Hainan 571101, China.
authors.forename: Bingjun
authors.initials: B
authors.lastname: Han
mesh_terms: D007477:Ions; D013058:Mass Spectrometry; D008670:Metals / Q000633:toxicity*; D008872:Microwaves; D037742:Nanotubes, Carbon / Q000737:chemistry / Q000648:ultrastructure; D012275:Oryza / Q000378:metabolism*; D012639:Seeds / Q000378:metabolism*; D052616:Solid Phase Extraction
chemical_list: D007477:Ions; D008670:Metals; D037742:Nanotubes, Carbon