title: Identifying species of symbiont bacteria from the human gut that, alone, can induce intestinal Th17 cells in mice.
abstract: Th17 cells accrue in the intestine in response to particular microbes. In rodents, segmented filamentous bacteria (SFB) induce intestinal Th17 cells, but analogously functioning microbes in humans remain undefined. Here, we identified human symbiont bacterial species, in particular Bifidobacterium adolescentis, that could, alone, induce Th17 cells in the murine intestine. Similar to SFB, B. adolescentis was closely associated with the gut epithelium and engendered cognate Th17 cells without attendant inflammation. However, B. adolescentis elicited a transcriptional program clearly distinct from that of SFB, suggesting an alternative mechanism of promoting Th17 cell accumulation. Inoculation of mice with B. adolescentis exacerbated autoimmune arthritis in the K/BxN mouse model. Several off-the-shelf probiotic preparations that include Bifidobacterium strains also drove intestinal Th17 cell accumulation.
authors.affiliation: Department of Microbiology and Immunobiology, Harvard Medical School, Boston, MA 02115.
authors.forename: Tze Guan
authors.initials: TG
authors.lastname: Tan
authors.affiliation: Department of Microbiology and Immunobiology, Harvard Medical School, Boston, MA 02115.
authors.forename: Esen
authors.initials: E
authors.lastname: Sefik
authors.affiliation: Department of Microbiology and Immunobiology, Harvard Medical School, Boston, MA 02115.
authors.forename: Naama
authors.initials: N
authors.lastname: Geva-Zatorsky
authors.affiliation: Department of Microbiology and Immunobiology, Harvard Medical School, Boston, MA 02115.
authors.forename: Lindsay
authors.initials: L
authors.lastname: Kua
authors.affiliation: Department of Immunobiology, University of Arizona, Tucson, AZ 85719.
authors.forename: Debdut
authors.initials: D
authors.lastname: Naskar
authors.affiliation: Department of Immunobiology, University of Arizona, Tucson, AZ 85719.
authors.forename: Fei
authors.initials: F
authors.lastname: Teng
authors.affiliation: Department of Microbiology and Immunobiology, Harvard Medical School, Boston, MA 02115.
authors.forename: Lesley
authors.initials: L
authors.lastname: Pasman
authors.affiliation: Department of Microbiology and Immunobiology, Harvard Medical School, Boston, MA 02115.
authors.forename: Adriana
authors.initials: A
authors.lastname: Ortiz-Lopez
authors.affiliation: UCB Pharma, Slough, Berkshire SL1 3WE, United Kingdom.
authors.forename: Ray
authors.initials: R
authors.lastname: Jupp
authors.affiliation: Department of Immunobiology, University of Arizona, Tucson, AZ 85719.|Arizona Arthritis Center, College of Medicine, University of Arizona, Tucson, AZ 85719.
authors.forename: Hsin-Jung Joyce
authors.initials: HJ
authors.lastname: Wu
authors.affiliation: Department of Microbiology and Immunobiology, Harvard Medical School, Boston, MA 02115.
authors.forename: Dennis L
authors.initials: DL
authors.lastname: Kasper
authors.affiliation: Department of Microbiology and Immunobiology, Harvard Medical School, Boston, MA 02115; cbdm@hms.harvard.edu dm@hms.harvard.edu.
authors.forename: Christophe
authors.initials: C
authors.lastname: Benoist
authors.affiliation: Department of Microbiology and Immunobiology, Harvard Medical School, Boston, MA 02115; cbdm@hms.harvard.edu dm@hms.harvard.edu.
authors.forename: Diane
authors.initials: D
authors.lastname: Mathis
mesh_terms: D000818:Animals; D001169:Arthritis, Experimental / Q000209:etiology / Q000276:immunology / Q000382:microbiology; D000070005:Bifidobacterium adolescentis / Q000276:immunology* / Q000302:isolation & purification; D005260:Female; D000069196:Gastrointestinal Microbiome / Q000276:immunology*; D020869:Gene Expression Profiling; D005856:Germ-Free Life / Q000235:genetics / Q000276:immunology; D006801:Humans; D018928:Immunity, Mucosal; D015212:Inflammatory Bowel Diseases / Q000276:immunology / Q000382:microbiology / Q000473:pathology; D007413:Intestinal Mucosa / Q000166:cytology* / Q000276:immunology / Q000382:microbiology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D019936:Probiotics; D013559:Symbiosis / Q000235:genetics / Q000276:immunology; D058504:Th17 Cells / Q000166:cytology / Q000276:immunology*
keywords: Th17 cells; intestine; microbiota; mucosal immunology; probiotic