title: New insights into the role of the porcine intestinal yeast, Kazachstania slooffiae, in intestinal environment of weaned piglets.
abstract: Kazachstania slooffiae is a porcine intestinal yeast whose role in the intestinal environment is largely unexplored. Therefore, the impact of K. slooffiae on growth performance, intestinal microbial metabolites and the microbiota of weaned piglets was investigated in this study. Forty-eight German Landrace pigs were weaned at day 27 or 28 of life and grouped into one control and three treatment groups. During the 5-week experiment, piglets had ad libitum access to feed and water. On days 5, 6 and 7 post weaning, pigs were orally supplemented with either placebo or K. slooffiae cells once a day. Faecal samples collected on days 5-8, 14, 21 and 28 post weaning were used for microbiological and chemical analyses. Between groups, there were no significant differences in the incidence of diarrhoea, pH and growth performance. Total yeasts and K. slooffiae correlated positively with total short-chain fatty acids, acetic, propionic, n-butyric, i-valeric and valeric acids, and negatively with pH. Pyrosequencing of the bacterial intestinal community revealed that K. slooffiae significantly affected the composition of the microbiota. The results of this study suggest that K. slooffiae may play an important role in the porcine digestive system, especially in the critical weaning period.
authors.affiliation: Institute of Agricultural and Nutritional Sciences, Group Animal Nutrition, Martin Luther University Halle-Wittenberg, 06120 Halle (Saale), Germany urubschurov@web.de.
authors.forename: Vladimir
authors.initials: V
authors.lastname: Urubschurov
authors.affiliation: Chair of Nutrition Physiology and Animal Nutrition, University of Rostock, 18059 Rostock, Germany.
authors.forename: Kirsten
authors.initials: K
authors.lastname: Büsing
authors.affiliation: Institute for Genetics and Biometry, Leibniz Institute for Farm Animal Biology (FBN), 18196 Dummerstorf, Germany.
authors.forename: Gertraude
authors.initials: G
authors.lastname: Freyer
authors.affiliation: Biological Oceanography, Leibniz Institute for Baltic Sea Research, Warnemünde (IOW), Rostock, 18119 Rostock, Germany.
authors.forename: Daniel P R
authors.initials: DP
authors.lastname: Herlemann
authors.affiliation: Institute of Agricultural and Nutritional Sciences, Group Animal Nutrition, Martin Luther University Halle-Wittenberg, 06120 Halle (Saale), Germany.|Chair of Nutrition Physiology and Animal Nutrition, University of Rostock, 18059 Rostock, Germany.
authors.forename: Wolfgang-Bernhard
authors.initials: WB
authors.lastname: Souffrant
authors.affiliation: Institute of Agricultural and Nutritional Sciences, Group Animal Nutrition, Martin Luther University Halle-Wittenberg, 06120 Halle (Saale), Germany.
authors.forename: Annette
authors.initials: A
authors.lastname: Zeyner
mesh_terms: D000821:Animal Feed / Q000032:analysis; D000818:Animals; D003967:Diarrhea; D019587:Dietary Supplements; D004064:Digestive System; D005232:Fatty Acids, Volatile; D005243:Feces / Q000382:microbiology; D007422:Intestines / Q000382:microbiology*; D064307:Microbiota; D012441:Saccharomyces cerevisiae; D004718:Saccharomycetales / Q000502:physiology*; D013552:Swine / Q000382:microbiology*; D014886:Weaning
chemical_list: D005232:Fatty Acids, Volatile
keywords: 454 pyrosequencing; SCFA; intestinal metabolites; microbita; yeast supplementation; yeast-bacteria interaction