title: Report From the First and Second Spanish Killer Immunoglobulin-Like Receptor Genotyping Workshops: External Quality Control for Natural Killer Alloreactive Donor Selection in Haploidentical Stem Cell Transplantation.
abstract: An important factor affecting the success in the setting of related haploidentical hematopoietic stem cell transplantation (HSCT) is the graft-versus-leukemia effect mediated by natural killer (NK) cells when the donor displays NK alloreactivity versus the recipient. NK cell function is regulated by killer immunoglobulin-like receptors (KIR) and it has been described that donor KIR genotype influences transplantation outcome. This has led to a requirement of laboratories to have a quality assurance program for validation and control of their KIR genotyping methods. The goal of the 1st and 2nd Spanish KIR Genotyping Workshops was to provide an external proficiency testing program in KIR genotyping for Spanish immunology and transplant laboratories. These workshops were conducted during the years 2014-2016 and consisted of 17 participating laboratories typing a set of 20 samples. The presence/absence of 16 mandatory KIR loci (2DL1, 2DL2, 2DL3, 2DL4, 2DL5, 2DS1, 2DS2, 2DS3, 2DS4, 2DS5, 2DP1, 3DL1, 3DL2, 3DL3, 3DS1, and 3DP1) was evaluated per sample. Methods for KIR genotyping included polymerase chain reaction with the use of sequence-specific primers and sequence-specific oligoprobes. Consensus typing was reached in all samples, and the performance of laboratories in external proficiency testing was satisfactory in all cases. The polymorphism detected in the small sample studied in both workshops is indicative of an ample variety of KIR gene profiles in the Spanish population.
authors.affiliation: Histocompatibility, Centro de Transfusión de la Comunidad Valenciana, Valencia, Spain. Electronic address: planelles_dol@gva.es.
authors.forename: D
authors.initials: D
authors.lastname: Planelles
authors.affiliation: Immunogenetics and Histocompatibility, Instituto de Investigación Sanitaria Puerta de Hierro, Madrid, Spain.
authors.forename: C
authors.initials: C
authors.lastname: Vilches
authors.affiliation: Immunology, Hospital Universitario Virgen del Rocío, Sevilla, Spain.
authors.forename: F
authors.initials: F
authors.lastname: González-Escribano
authors.affiliation: Immunology, Hospital Clínico Universitario Virgen de la Arrixaca, Murcia, Spain.
authors.forename: M
authors.initials: M
authors.lastname: Muro
authors.affiliation: Immunology, Hospital Universitario Reina Sofía, Córdoba, Spain.
authors.forename: R
authors.initials: R
authors.lastname: González-Fernández
authors.affiliation: Immunology, Hospital Universitario de Gran Canaria Dr Negrín, Las Palmas de Gran Canaria, Spain.
authors.forename: F
authors.initials: F
authors.lastname: Sánchez
authors.affiliation: Immunology, Hospital Universitario Marqués de Valdecilla, Santander, Spain.
authors.forename: J
authors.initials: J
authors.lastname: Gonzalo Ocejo
authors.affiliation: Molecular Biology and Histocompatibility, Centro de Transfusión de Galicia, Santiago de Compostela, Spain.
authors.forename: A
authors.initials: A
authors.lastname: Eiras
authors.affiliation: Histocompatibility and Immunogenetics, Banc de Sang i Teixits, Barcelona, Spain.
authors.forename: J L
authors.initials: JL
authors.lastname: Caro
authors.affiliation: Immunology, Hospital Clínic Universitari, Barcelona, Spain.
authors.forename: E
authors.initials: E
authors.lastname: Palou
authors.affiliation: Immunology, Hospital Clínico Universitario Virgen de la Arrixaca, Murcia, Spain.
authors.forename: J A
authors.initials: JA
authors.lastname: Campillo
authors.affiliation: Immunology, Hospital Universitario Donostia, San Sebastián, Spain.
authors.forename: M D
authors.initials: MD
authors.lastname: de Juan
authors.affiliation: Immunology, Complejo Hospitalario Universitario Insular Materno-Infantil, Las Palmas de Gran Canaria, Spain.
authors.forename: O
authors.initials: O
authors.lastname: Montes
authors.affiliation: Histocompatibility, Centro de Transfusión de la Comunidad de Madrid, Madrid, Spain.
authors.forename: A
authors.initials: A
authors.lastname: Balas
authors.affiliation: Molecular Biology-Hematology, Hospital Clínico Universitario, Salamanca, Spain.
authors.forename: L
authors.initials: L
authors.lastname: Marín
authors.affiliation: Immunology, Hospital Regional Universitario Carlos Haya, Málaga, Spain.
authors.forename: A
authors.initials: A
authors.lastname: Torío
authors.affiliation: Clinical Immunology, Hospital Clínico San Carlos, Madrid, Spain.
authors.forename: M
authors.initials: M
authors.lastname: Fernández-Arquero
authors.affiliation: Immunology and Genetics, Hospital Infanta Cristina, Badajoz, Spain.
authors.forename: C
authors.initials: C
authors.lastname: González-Roiz
authors.affiliation: Immunology, Hospital Universitario Central de Asturias, Oviedo, Spain.
authors.forename: A
authors.initials: A
authors.lastname: López-Vázquez
authors.affiliation: Immunogenetics and Histocompatibility, Instituto de Investigación Sanitaria Puerta de Hierro, Madrid, Spain.
authors.forename: E
authors.initials: E
authors.lastname: Cisneros
authors.affiliation: Immunology, Hospital Universitario Virgen del Rocío, Sevilla, Spain.
authors.forename: C
authors.initials: C
authors.lastname: Abad-Molina
authors.affiliation: Immunology, Hospital Universitario de Gran Canaria Dr Negrín, Las Palmas de Gran Canaria, Spain.
authors.forename: R
authors.initials: R
authors.lastname: López
authors.affiliation: Molecular Biology and Histocompatibility, Centro de Transfusión de Galicia, Santiago de Compostela, Spain.
authors.forename: M L
authors.initials: ML
authors.lastname: Abad-Alastruey
authors.affiliation: Immunology, Hospital Clínic Universitari, Barcelona, Spain.
authors.forename: C
authors.initials: C
authors.lastname: Serra
authors.affiliation: Immunology, Hospital Clínico Universitario Virgen de la Arrixaca, Murcia, Spain.
authors.forename: A M
authors.initials: AM
authors.lastname: García-Alonso
authors.affiliation: Histocompatibility, Centro de Transfusión de la Comunidad de Madrid, Madrid, Spain.
authors.forename: J L
authors.initials: JL
authors.lastname: Vicario
mesh_terms: D046148:Donor Selection / Q000379:methods*; D005787:Gene Frequency; D005838:Genotype; D018380:Hematopoietic Stem Cell Transplantation / Q000379:methods*; D006801:Humans; D007694:Killer Cells, Natural / Q000276:immunology; D016133:Polymerase Chain Reaction / Q000379:methods; D011110:Polymorphism, Genetic; D011786:Quality Control; D054340:Receptors, KIR / Q000235:genetics*
chemical_list: D054340:Receptors, KIR