title: The complete chloroplast genome sequence ofHelwingia himalaica(Helwingiaceae, Aquifoliales) and a chloroplast phylogenomic analysis of the Campanulidae.
abstract: Complete chloroplast genome sequences have been very useful for understanding phylogenetic relationships in angiosperms at the family level and above, but there are currently large gaps in coverage. We report the chloroplast genome forHelwingia himalaica, the first in the distinctive family Helwingiaceae and only the second genus to be sequenced in the order Aquifoliales. We then combine this with 36 published sequences in the large (c. 35,000 species) subclass Campanulidae in order to investigate relationships at the order and family levels. TheHelwingiagenome consists of 158,362 bp containing a pair of inverted repeat (IR) regions of 25,996 bp separated by a large single-copy (LSC) region and a small single-copy (SSC) region which are 87,810 and 18,560 bp, respectively. There are 142 known genes, including 94 protein-coding genes, eight ribosomal RNA genes, and 40 tRNA genes. The topology of the phylogenetic relationships between Apiales, Asterales, and Dipsacales differed between analyses based on complete genome sequences and on 36 shared protein-coding genes, showing that further studies of campanulid phylogeny are needed.
authors.affiliation: Center for Integrative Conservation, Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, Xishuangbanna, Yunnan, China; University of Chinese Academy of Sciences, Beijing, Beijing, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Yao
authors.affiliation: Key Laboratory of Dai and Southern medicine of Xishuangbanna Dai Autonomous Prefecture, Yunnan Branch Institute of Medicinal Plant, Chinese Academy of Medical Sciences , Jinghong, Yunnan , China.
authors.forename: Ying-Ying
authors.initials: YY
authors.lastname: Liu
authors.affiliation: Center for Integrative Conservation, Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences , Xishuangbanna, Yunnan , China.
authors.forename: Yun-Hong
authors.identifier: 0000-0001-6238-2743
authors.initials: YH
authors.lastname: Tan
authors.affiliation: Center for Integrative Conservation, Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, Xishuangbanna, Yunnan, China; University of Chinese Academy of Sciences, Beijing, Beijing, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Song
authors.affiliation: Center for Integrative Conservation, Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences , Xishuangbanna, Yunnan , China.
authors.forename: Richard T
authors.initials: RT
authors.lastname: Corlett
keywords: Asterids; Campanulidae; Phylogeny; Plastomes; Yunnan