title: Liver steatosis in pre-transplant liver biopsies can be quantified rapidly and accurately by nuclear magnetic resonance analysis.
abstract: Donor livers marginally acceptable or acceptable according to extended criteria are more frequently transplanted due to the growing discrepancy between demand and availability of donor organs. One type of marginally acceptable graft is a steatotic donor liver, because it is more sensitive to ischemia-reperfusion injury. Thus, quantitative assessment of steatosis is crucial prior to liver transplantation. Extent of steatosis of 49 pre-reperfusion liver biopsies from patients who received orthotopic liver transplantation was assessed by three techniques: semi-quantitative histological evaluation, computerized histomorphometry, and NMR-based estimation of fat content. The findings were correlated to clinical data and to histological examination of corresponding post-reperfusion biopsies for quantification of ischemia-reperfusion injury. We found that values obtained through all three assessment methods were positively correlated. None of the values obtained by the three applied methods correlated with clinical outcome or extent of ischemia-reperfusion injury. Quantitative evaluation of steatosis by NMR yields results comparable to histological and morphometrical assessment. This technique is rapid (<5 min), accurately quantifies fat in donor livers, and provides results that can be used when evaluation by a pathologist is not available.
authors.affiliation: Institute of Pathology, University Hospital Essen, University of Duisburg-Essen, Hufelandstr. 55, 45147, Essen, Germany.
authors.forename: Stefanie
authors.initials: S
authors.lastname: Bertram
authors.affiliation: Institute of Pathology, University Hospital Essen, University of Duisburg-Essen, Hufelandstr. 55, 45147, Essen, Germany.
authors.forename: Cathrin
authors.initials: C
authors.lastname: Myland
authors.affiliation: Department of General, Visceral and Transplantation Surgery, University Hospital Essen, University of Duisburg-Essen, Hufelandstr. 55, 45147, Essen, Germany.
authors.forename: Sandra
authors.initials: S
authors.lastname: Swoboda
authors.affiliation: Department of General, Visceral and Transplantation Surgery, University Hospital Essen, University of Duisburg-Essen, Hufelandstr. 55, 45147, Essen, Germany.
authors.forename: Anja
authors.initials: A
authors.lastname: Gallinat
authors.affiliation: Department of General, Visceral and Transplantation Surgery, University Hospital Essen, University of Duisburg-Essen, Hufelandstr. 55, 45147, Essen, Germany.
authors.forename: Thomas
authors.initials: T
authors.lastname: Minor
authors.affiliation: Institute for Medical Informatics, Biometry and Epidemiology, University of Duisburg-Essen, Hufelandstr. 55, 45147, Essen, Germany.
authors.forename: Nils
authors.initials: N
authors.lastname: Lehmann
authors.affiliation: Institute of Pathology, University Hospital Essen, University of Duisburg-Essen, Hufelandstr. 55, 45147, Essen, Germany.
authors.forename: Michael
authors.initials: M
authors.lastname: Thie
authors.affiliation: Institute of Pathology, University Hospital Essen, University of Duisburg-Essen, Hufelandstr. 55, 45147, Essen, Germany.|Institute for Medical Informatics, Biometry and Epidemiology, University of Duisburg-Essen, Hufelandstr. 55, 45147, Essen, Germany.
authors.forename: Julia
authors.initials: J
authors.lastname: Kälsch
authors.affiliation: Institute of Pathology, University Hospital Essen, University of Duisburg-Essen, Hufelandstr. 55, 45147, Essen, Germany.
authors.forename: Leona
authors.initials: L
authors.lastname: Pott
authors.affiliation: Department of Gastroenterology and Hepatology, University Hospital Essen, University of Duisburg-Essen, Hufelandstr. 55, 45147, Essen, Germany.
authors.forename: Ali
authors.initials: A
authors.lastname: Canbay
authors.affiliation: Institute of Legal Medicine University Hospital Essen, University of Duisburg-Essen, Hufelandstr. 55, 45147, Essen, Germany.
authors.forename: Thomas
authors.initials: T
authors.lastname: Bajanowski
authors.affiliation: Institute of Pathology, University Hospital Essen, University of Duisburg-Essen, Hufelandstr. 55, 45147, Essen, Germany.
authors.forename: Henning
authors.initials: H
authors.lastname: Reis
authors.affiliation: Department of General, Visceral and Transplantation Surgery, University Hospital Essen, University of Duisburg-Essen, Hufelandstr. 55, 45147, Essen, Germany.
authors.forename: Andreas
authors.initials: A
authors.lastname: Paul
authors.affiliation: Institute of Pathology, University Hospital Essen, University of Duisburg-Essen, Hufelandstr. 55, 45147, Essen, Germany. hideo.baba@uk-essen.de.
authors.forename: Hideo A
authors.initials: HA
authors.lastname: Baba
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001706:Biopsy; D046148:Donor Selection; D005234:Fatty Liver / Q000150:complications / Q000175:diagnosis* / Q000473:pathology; D005260:Female; D006801:Humans; D008099:Liver / Q000473:pathology* / Q000601:surgery; D016031:Liver Transplantation; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D008875:Middle Aged; D017063:Outcome Assessment, Health Care; D015427:Reperfusion Injury / Q000209:etiology; D012307:Risk Factors; D055815:Young Adult
keywords: Ischemia-reperfusion injury; Liver transplantation; Steatosis