title: Effective interference between Simbu serogroup orthobunyaviruses in mammalian cells.
abstract: The Simbu serogroup of orthobunyaviruses comprises a wide range of viruses with different medical and veterinary relevance. These viruses are known to reassort, and coinfection of the same cell is one of the prerequisites for reassortment. Here, a mammalian cell line was infected with various members of this virus group, inoculated after several time points with a second Simbu serogroup virus, and analyzed by strain or species specific immunofluorescence staining. Different virus species or different strains of the same virus species were able to co-infect mammalian cells, but only for a limited time frame. After a few hours, the replication of the first virus led to a gradual inhibition of a second virus until a complete resistance to superinfection after 24h regardless whether it is another strain of the same virus species or a distinct member of the serogroup.
authors.affiliation: Institute of Diagnostic Virology, Friedrich-Loeffler-Institut (FLI), Suedufer 10, 17493 Greifswald - Insel Riems, Germany. Electronic address: kerstin.wernike@fli.de.
authors.forename: Kerstin
authors.initials: K
authors.lastname: Wernike
authors.affiliation: Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia Romagna, Via Bianchi 7, 25125 Brescia, Italy.
authors.forename: Emiliana
authors.initials: E
authors.lastname: Brocchi
authors.affiliation: Institute of Diagnostic Virology, Friedrich-Loeffler-Institut (FLI), Suedufer 10, 17493 Greifswald - Insel Riems, Germany.
authors.forename: Martin
authors.initials: M
authors.lastname: Beer
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D002044:Bunyaviridae Infections / Q000821:virology*; D002460:Cell Line; D060085:Coinfection; D006224:Cricetinae; D065288:Serogroup; D012840:Simbu virus / Q000276:immunology* / Q000502:physiology; D013045:Species Specificity; D014779:Virus Replication
chemical_list: D000911:Antibodies, Monoclonal
keywords: Coinfection; Interaction; Interference; Orthobunyavirus; Reassortment; Schmallenberg virus