title: No Role for Mast Cells in Obesity-Related Metabolic Dysregulation.
abstract: Obesity-related adipose tissue (AT) inflammation that promotes metabolic dysregulation is associated with increased AT mast cell numbers. Mast cells are potent inducers of inflammatory responses and could potentially contribute to obesity-induced AT inflammation and metabolic dysregulation. Conflicting findings were reported on obesity-related metabolic dysfunction in mast cell-deficient mice, thus creating a controversy that has not been resolved to date. Whereas traditionalKithypomorphic mast cell-deficient strains featured reduced diet-induced obesity and diabetes, aKit-independent model of mast cell deficiency, Cpa3Cre/+mice, displayed no alterations in obesity and insulin sensitivity. Herein, we analyzed diet-induced obesity inMcpt5-Cre R-DTAmice, in which the lack of mast cells is caused by a principle different from mast cell deficiency in Cpa3Cre/+mice orKitmutations. We observed no difference between mast cell-deficient and -proficient mice in diet-induced obesity with regards to weight gain, glucose tolerance, insulin resistance, metabolic parameters, hepatic steatosis, and AT or liver inflammation. We conclude that mast cells play no essential role in obesity and related pathologies.
authors.affiliation: Department of Clinical Pathobiochemistry, Medical Faculty, Technische Universität Dresden, Dresden, Germany; Faculty of Science, University of South Bohemia in České Budějovice, České Budějovice, Czech Republic.
authors.forename: Jindřich
authors.initials: J
authors.lastname: Chmelař
authors.affiliation: Department of Clinical Pathobiochemistry, Medical Faculty, Technische Universität Dresden, Dresden, Germany; Medical Faculty, Institute of Clinical Chemistry and Laboratory Medicine, Technische Universität Dresden, Dresden, Germany.
authors.forename: Antonios
authors.initials: A
authors.lastname: Chatzigeorgiou
authors.affiliation: Department of Clinical Pathobiochemistry, Medical Faculty, Technische Universität Dresden, Dresden, Germany; Paul Langerhans Institute Dresden of the Helmholtz Center Munich at University Hospital and Faculty of Medicine, TU Dresden, Dresden, Germany; German Center for Diabetes Research (DZD e.V.), Neuherberg, Germany.
authors.forename: Kyoung-Jin
authors.initials: KJ
authors.lastname: Chung
authors.affiliation: Department of Clinical Pathobiochemistry, Medical Faculty, Technische Universität Dresden , Dresden , Germany.
authors.forename: Marta
authors.initials: M
authors.lastname: Prucnal
authors.affiliation: Department of Infection Biology, Universitätsklinikum Erlangen at the Friedrich-Alexander Universität Erlangen-Nürnberg (FAU) , Erlangen , Germany.
authors.forename: David
authors.initials: D
authors.lastname: Voehringer
authors.affiliation: Institute for Immunology, Technische Universität Dresden , Dresden , Germany.
authors.forename: Axel
authors.initials: A
authors.lastname: Roers
authors.affiliation: Department of Clinical Pathobiochemistry, Medical Faculty, Technische Universität Dresden, Dresden, Germany; Medical Faculty, Institute of Clinical Chemistry and Laboratory Medicine, Technische Universität Dresden, Dresden, Germany; Paul Langerhans Institute Dresden of the Helmholtz Center Munich at University Hospital and Faculty of Medicine, TU Dresden, Dresden, Germany; German Center for Diabetes Research (DZD e.V.), Neuherberg, Germany.
authors.forename: Triantafyllos
authors.initials: T
authors.lastname: Chavakis
keywords: diet-induced obesity; glucose tolerance; insulin resistance; mast cell deficiency; metabolic dysregulation