title: Endothelial-Mesenchymal Transition in Vascular Calcification of Ins2Akita/+ Mice.
abstract: Endothelial-mesenchymal transition (EndMT) drives endothelium to contribute to normal development and disease processes. Here, we report that EndMTs occur in the diabetic endothelium of Ins2Akita/wt mouse, and show that induction of sex determining region Y-box 2 (Sox2) is a mediator of excess BMP signaling that results in activation of EndMTs and increased vascular calcification. We also find an induction of a complex of serine proteases in the diabetic endothelium, required for the up-regulation of Sox2. Our results suggest that EndMTs contribute to vascular calcification in diabetic arteries.
authors.affiliation: Division of Cardiology, David Geffen School of Medicine at UCLA, Los Angeles, California, United States of America.
authors.forename: Pierre J
authors.initials: PJ
authors.lastname: Guihard
authors.affiliation: Division of Cardiology, David Geffen School of Medicine at UCLA, Los Angeles, California, United States of America.
authors.forename: Jiayi
authors.initials: J
authors.lastname: Yao
authors.affiliation: Division of Cardiology, David Geffen School of Medicine at UCLA, Los Angeles, California, United States of America.
authors.forename: Ana M
authors.initials: AM
authors.lastname: Blazquez-Medela
authors.affiliation: The Molecular Biology Institute at UCLA, Los Angeles, California, United States of America.|Jonsson Comprehensive Cancer Center at UCLA, Los Angeles, California, United States of America.
authors.forename: Luisa
authors.initials: L
authors.lastname: Iruela-Arispe
authors.affiliation: Division of Cardiology, David Geffen School of Medicine at UCLA, Los Angeles, California, United States of America.|The Molecular Biology Institute at UCLA, Los Angeles, California, United States of America.
authors.forename: Kristina I
authors.initials: KI
authors.lastname: Boström
authors.affiliation: Division of Cardiology, David Geffen School of Medicine at UCLA, Los Angeles, California, United States of America.|Jonsson Comprehensive Cancer Center at UCLA, Los Angeles, California, United States of America.
authors.forename: Yucheng
authors.initials: Y
authors.lastname: Yao
mesh_terms: D000818:Animals; D001808:Blood Vessels / Q000473:pathology*; D002114:Calcinosis / Q000473:pathology*; D003921:Diabetes Mellitus, Experimental / Q000235:genetics; D004730:Endothelium, Vascular / Q000201:enzymology / Q000473:pathology*; D007328:Insulin / Q000235:genetics*; D008648:Mesoderm / Q000473:pathology*; D051379:Mice; D008822:Mice, Transgenic; D055748:SOXB1 Transcription Factors / Q000502:physiology; D057057:Serine Proteases / Q000378:metabolism; D015854:Up-Regulation
chemical_list: C561967:Ins2 protein, mouse; D007328:Insulin; D055748:SOXB1 Transcription Factors; C527783:Sox2 protein, mouse; D057057:Serine Proteases