title: Long-term Leucine Supplementation Improves Metabolic But Not Molecular Responses in the Skeletal Muscle of Trained Rats Submitted to Exhaustive Exercise.
abstract: Although there is some evidence of an ergogenic effect of leucine supplementation on acute response to exercise, there is a paucity of information on whether long-term leucine supplementation influences the adaptive response to chronic endurance training and performance. The main aim of our study was to assess the role of long-term leucine supplementation on molecular and metabolic response in skeletal muscle of trained rats after an exhaustion test.
  
  
  
  Twenty-four male Wistar rats were randomly allocated into 4 groups. Two of them (control and trained groups) received a balanced control diet (18% protein) and the other 2 (control leucine and trained leucine groups) received a leucine-rich diet (15% protein with 3% leucine) for 6 weeks. The trained groups were submitted to 1 hour of swimming exercise, 5 d/wk for 6 weeks. Three days after the exercise training period, trained groups were submitted to swimming exercise until exhaustion and muscle metabolic and molecular parameters were assessed.
  
  
  
  Endurance training increased citrate synthase activity significantly, whereas exercise until exhaustion increased cytokine levels and led to a lack of activation of phosphorylation of the signaling intermediates assessed. Long-term leucine supplementation enhanced muscle glycogen level in trained rats and citrate synthase activity in sedentary ones. However, it failed to enhance endurance performance of trained rats submitted to an exhaustion test and did not prevent exercise-induced reduction in Akt and mTOR activation.
  
  
  
  Long-term leucine supplementation can enhance citrate synthase activity by itself in sedentary individuals and glycogen content when combined with exercise; however, it does not improve endurance performance or prevent Akt and mTOR exercise-induced inhibition.
authors.affiliation: a Department of Structural and Functional Biology , Institute of Biology, University of Campinas (UNICAMP) , Campinas , São Paulo , BRAZIL.|b Sport Science Department , Faculty of Physical Education, Metropolitan College of Campinas (Metrocamp) , Campinas, São Paulo , BRAZIL.
authors.forename: Gustavo Barbosa Dos
authors.initials: GB
authors.lastname: Santos
authors.affiliation: a Department of Structural and Functional Biology , Institute of Biology, University of Campinas (UNICAMP) , Campinas , São Paulo , BRAZIL.
authors.forename: André Gustavo de
authors.initials: AG
authors.lastname: Oliveira
authors.affiliation: a Department of Structural and Functional Biology , Institute of Biology, University of Campinas (UNICAMP) , Campinas , São Paulo , BRAZIL.
authors.forename: Maria Cristina Cintra
authors.initials: MC
authors.lastname: Gomes Marcondes
authors.affiliation: a Department of Structural and Functional Biology , Institute of Biology, University of Campinas (UNICAMP) , Campinas , São Paulo , BRAZIL.
authors.forename: Miguel Arcanjo
authors.initials: MA
authors.lastname: Areas
mesh_terms: D000818:Animals; D004032:Diet; D019587:Dietary Supplements; D004734:Energy Metabolism / Q000187:drug effects*; D005221:Fatigue; D007930:Leucine / Q000008:administration & dosage* / Q000494:pharmacology*; D008297:Male; D018482:Muscle, Skeletal / Q000187:drug effects* / Q000502:physiology; D010805:Physical Conditioning, Animal / Q000502:physiology*; D051381:Rats; D017208:Rats, Wistar; D015430:Weight Gain
chemical_list: D007930:Leucine
keywords: endurance performance; leucine; skeletal muscle