title: A novel mutation in the fibrinogen Aα chain (Gly13Arg, fibrinogen Nanning) causes congenital dysfibrinogenemia associated with defective peptide A release.
abstract: Dysfibrinogenemia is characterized by blood coagulation dysfunction induced by an abnormal molecular structure of fibrinogen. Here, we describe a new case. A 32-year-old female was suspected of having dysfibrinogenemia during routine laboratory screening, based on her decreased functional fibrinogen level, normal fibrinogen antigen level, and prolonged thrombin time. We extracted DNA and performed polymerase chain reaction and DNA sequencing to identify genetic mutation. Fibrin polymerization, the kinetics of the fibrinopeptide release, scanning electron microscopy, mass spectrometric analysis, fibrin cross-linking, sodium dodecyl sulfate polyacrylamide gel electrophoresis and western blot were conducted. DNA sequencing identified a heterozygous point mutation, Gly13Arg in Aα chain. Fibrin polymerization was markedly impaired (prolonged lag phase and decreased final turbidity). The rate and extent of fibrinopeptide A release from the patient were abnormal and reduced. The mass spectrometry analysis revealed the presence of mutant fibrinogen chains in the patient's circulation. Electron micrographs revealed abnormal fibrin clots. Fibrin cross-linking was normal. Sodium dodecyl sulfate polyacrylamide gel electrophoresis and western blot showed no difference. We report a new case with a mutation in the fibrinopeptide A region, AαGly13Arg. These results indicated that the functional abnormalities were related to delayed and defective fibrinopeptide A cleavage and likely impaired thrombin binding.
authors.affiliation: Department of Clinical Laboratory, The First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Yan
authors.affiliation: Department of Clinical Laboratory, The First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi, China.
authors.forename: Meiling
authors.initials: M
authors.lastname: Luo
authors.affiliation: Department of Hematology, The First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Cheng
authors.affiliation: Department of Clinical Laboratory, The First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Liao
authors.affiliation: Department of Clinical Laboratory, The First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi, China.
authors.forename: Xuelian
authors.initials: X
authors.lastname: Deng
authors.affiliation: Department of Hematology, The First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi, China. ddh_gx@163.com.
authors.forename: Donghong
authors.initials: D
authors.lastname: Deng
authors.affiliation: Department of Clinical Laboratory, The First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi, China. fqlin1998@163.com.
authors.forename: Faquan
authors.initials: F
authors.lastname: Lin
mesh_terms: D000328:Adult; D000347:Afibrinogenemia / Q000235:genetics*; D001780:Blood Coagulation Tests; D005260:Female; D005337:Fibrin / Q000378:metabolism; D005340:Fibrinogen / Q000235:genetics*; D015241:Fibrinogens, Abnormal / Q000235:genetics; D005344:Fibrinopeptide A / Q000378:metabolism*; D006801:Humans; D017354:Point Mutation / Q000235:genetics*; D017422:Sequence Analysis, DNA
chemical_list: D015241:Fibrinogens, Abnormal; C464794:fibrinogen Aalpha; D005344:Fibrinopeptide A; D005337:Fibrin; D005340:Fibrinogen
keywords: Dysfibrinogenemia; Fibrinogen; Mutation