title: Multiplex gene editing by CRISPR-Cpf1 using a single crRNA array.
abstract: Targeting of multiple genomic loci with Cas9 is limited by the need for multiple or large expression constructs. Here we show that the ability of Cpf1 to process its own CRISPR RNA (crRNA) can be used to simplify multiplexed genome editing. Using a single customized CRISPR array, we edit up to four genes in mammalian cells and three in the mouse brain, simultaneously.
authors.affiliation: Broad Institute of MIT and Harvard, Cambridge, MA 02142.|McGovern Institute for Brain Research, Massachusetts Institute of Technology, Cambridge, MA 02139.|Department of Brain and Cognitive Sciences, Massachusetts Institute of Technology, Cambridge, MA 02139.|Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139.|Department of Developmental Pathology, Institute of Pathology, Bonn Medical School, Sigmund Freud Street 25, 53127 Bonn, Germany.
authors.forename: Bernd
authors.initials: B
authors.lastname: Zetsche
authors.affiliation: Broad Institute of MIT and Harvard, Cambridge, MA 02142.|McGovern Institute for Brain Research, Massachusetts Institute of Technology, Cambridge, MA 02139.|Department of Brain and Cognitive Sciences, Massachusetts Institute of Technology, Cambridge, MA 02139.|Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139.
authors.forename: Matthias
authors.initials: M
authors.lastname: Heidenreich
authors.affiliation: Laboratory of Microbiology, Department of Agrotechnology and Food Sciences, Wageningen University, Stippeneng 4, 6708 WE Wageningen, Netherlands.
authors.forename: Prarthana
authors.initials: P
authors.lastname: Mohanraju
authors.affiliation: Broad Institute of MIT and Harvard, Cambridge, MA 02142.|McGovern Institute for Brain Research, Massachusetts Institute of Technology, Cambridge, MA 02139.|Department of Brain and Cognitive Sciences, Massachusetts Institute of Technology, Cambridge, MA 02139.|Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139.|Skolkovo Institute of Science and Technology, Skolkovo, 143025, Russia.|Peter the Great St.Petersburg Polytechinc University, St. Petersburg, 195251, Russia.
authors.forename: Iana
authors.initials: I
authors.lastname: Fedorova
authors.affiliation: Broad Institute of MIT and Harvard, Cambridge, MA 02142.|Laboratory of Microbiology, Department of Agrotechnology and Food Sciences, Wageningen University, Stippeneng 4, 6708 WE Wageningen, Netherlands.
authors.forename: Jeroen
authors.initials: J
authors.lastname: Kneppers
authors.affiliation: Broad Institute of MIT and Harvard, Cambridge, MA 02142.|Harvard-MIT Division of Health Sciences and Technology, Massachusetts Institute of Technology, Cambridge, MA 02139.
authors.forename: Ellen M
authors.initials: EM
authors.lastname: DeGennaro
authors.affiliation: Broad Institute of MIT and Harvard, Cambridge, MA 02142.|McGovern Institute for Brain Research, Massachusetts Institute of Technology, Cambridge, MA 02139.|Department of Brain and Cognitive Sciences, Massachusetts Institute of Technology, Cambridge, MA 02139.|Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139.
authors.forename: Nerges
authors.initials: N
authors.lastname: Winblad
authors.affiliation: Broad Institute of MIT and Harvard, Cambridge, MA 02142.|McGovern Institute for Brain Research, Massachusetts Institute of Technology, Cambridge, MA 02139.|Department of Brain and Cognitive Sciences, Massachusetts Institute of Technology, Cambridge, MA 02139.|Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139.
authors.forename: Sourav R
authors.initials: SR
authors.lastname: Choudhury
authors.affiliation: Broad Institute of MIT and Harvard, Cambridge, MA 02142.|McGovern Institute for Brain Research, Massachusetts Institute of Technology, Cambridge, MA 02139.|Department of Brain and Cognitive Sciences, Massachusetts Institute of Technology, Cambridge, MA 02139.|Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139.|Harvard-MIT Division of Health Sciences and Technology, Massachusetts Institute of Technology, Cambridge, MA 02139.
authors.forename: Omar O
authors.initials: OO
authors.lastname: Abudayyeh
authors.affiliation: Broad Institute of MIT and Harvard, Cambridge, MA 02142.|McGovern Institute for Brain Research, Massachusetts Institute of Technology, Cambridge, MA 02139.|Department of Brain and Cognitive Sciences, Massachusetts Institute of Technology, Cambridge, MA 02139.|Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139.|Department of Systems Biology, Harvard Medical School, Boston, MA 02115.
authors.forename: Jonathan S
authors.initials: JS
authors.lastname: Gootenberg
authors.affiliation: Laboratory of Microbiology, Department of Agrotechnology and Food Sciences, Wageningen University, Stippeneng 4, 6708 WE Wageningen, Netherlands.
authors.forename: Wen Y
authors.initials: WY
authors.lastname: Wu
authors.affiliation: Broad Institute of MIT and Harvard, Cambridge, MA 02142.|McGovern Institute for Brain Research, Massachusetts Institute of Technology, Cambridge, MA 02139.
authors.forename: David A
authors.initials: DA
authors.lastname: Scott
authors.affiliation: Skolkovo Institute of Science and Technology, Skolkovo, 143025, Russia.|Waksman Institute for Microbiology, Rutgers, The State University of New Jersey, Piscataway, NJ 08854, USA.|Institute of Molecular Genetics, Russian Academy of Sciences, Moscow, 123182, Russia.
authors.forename: Konstantin
authors.initials: K
authors.lastname: Severinov
authors.affiliation: Laboratory of Microbiology, Department of Agrotechnology and Food Sciences, Wageningen University, Stippeneng 4, 6708 WE Wageningen, Netherlands.
authors.forename: John
authors.initials: J
authors.lastname: van der Oost
authors.affiliation: Broad Institute of MIT and Harvard, Cambridge, MA 02142.|McGovern Institute for Brain Research, Massachusetts Institute of Technology, Cambridge, MA 02139.|Department of Brain and Cognitive Sciences, Massachusetts Institute of Technology, Cambridge, MA 02139.|Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139.
authors.forename: Feng
authors.initials: F
authors.lastname: Zhang
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics*; D064130:CRISPR-Associated Proteins / Q000235:genetics*; D064113:CRISPR-Cas Systems / Q000235:genetics*; D000072669:Gene Editing / Q000379:methods*; D051379:Mice; D012329:RNA, Bacterial / Q000235:genetics*; D017423:Sequence Analysis, RNA / Q000379:methods*
chemical_list: D001426:Bacterial Proteins; D064130:CRISPR-Associated Proteins; D012329:RNA, Bacterial