title: Describing the Stem Cell Potency: The Various Methods of Functional Assessment andIn silicoDiagnostics.
abstract: Stem cells are defined by their capabilities to self-renew and give rise to various types of differentiated cells depending on their potency. They are classified as pluripotent, multipotent, and unipotent as demonstrated through their potential to generate the variety of cell lineages. While pluripotent stem cells may give rise to all types of cells in an organism, Multipotent and Unipotent stem cells remain restricted to the particular tissue or lineages. The potency of these stem cells can be defined by using a number of functional assays along with the evaluation of various molecular markers. These molecular markers include diagnosis of transcriptional, epigenetic, and metabolic states of stem cells. Many reports are defining the particular set of different functional assays, and molecular marker used to demonstrate the developmental states and functional capacities of stem cells. The careful evaluation of all these methods could help in generating standard identifying procedures/markers for them.
authors.affiliation: Stem Cell Research Laboratory, Department of Biotechnology, Delhi Technological University Delhi, India.
authors.forename: Vimal K
authors.initials: VK
authors.lastname: Singh
authors.affiliation: Stem Cell Research Laboratory, Department of Biotechnology, Delhi Technological University Delhi, India.
authors.forename: Abhishek
authors.initials: A
authors.lastname: Saini
authors.affiliation: Stem Cell Research Laboratory, Department of Biotechnology, Delhi Technological University Delhi, India.
authors.forename: Manisha
authors.initials: M
authors.lastname: Kalsan
authors.affiliation: Stem Cell Research Laboratory, Department of Biotechnology, Delhi Technological University Delhi, India.
authors.forename: Neeraj
authors.initials: N
authors.lastname: Kumar
authors.affiliation: Department of Chemistry, University of Delhi Delhi, India.
authors.forename: Ramesh
authors.initials: R
authors.lastname: Chandra
keywords: EpiBlast; EpiSCs; chimera; iPSCs; naïve; pluripotency; primed; teratoma