title: Effects of Aerobic Growth on the Fatty Acid and Hydrocarbon Compositions of Geobacter bemidjiensis BemT.
abstract: Geobacter spp., regarded as strict anaerobes, have been reported to grow under aerobic conditions. To elucidate the role of fatty acids in aerobiosis of Geobacter spp., we studied the effect of aerobiosis on fatty acid composition and turnover in G. bemidjiensis BemT. G. bemidjiensis BemTwas grown under the following different culture conditions: anaerobic culture for 4 days (type 1) and type 1 culture followed by 2-day anaerobic (type 2) or aerobic culture (anaerobic-to-aerobic shift; type 3). The mean cell weight of the type 3 culture was approximately 2.5-fold greater than that of type 1 and 2 cultures. The fatty acid methyl ester and hydrocarbon fraction contained hexadecanoic (16:0), 9-cis-hexadecenoic [16:1(9c)], tetradecanoic (14:0), tetradecenoic [14:1(7c)] acids, hentriacontanonaene, and hopanoids, but not long-chain polyunsaturated fatty acids. The type 3 culture contained higher levels of 14:0 and 14:1(7c) and lower levels of 16:0 and 16:1(9c) compared with type 1 and 2 cultures. The weight ratio of extracted lipid per dry cell was lower in the type 3 culture than in the type 1 and 2 cultures. We concluded that anaerobically-grown G. bemidjiensis BemTfollowed by aerobiosis were enhanced in growth, fatty acid turnover, and de novo fatty acid synthesis.
authors.affiliation: Horonobe Research Institute for the Subsurface Environment (H-RISE), NOASTEC.
authors.forename: Akio
authors.initials: A
authors.lastname: Ueno
authors.forename: Satoru
authors.initials: S
authors.lastname: Shimizu
authors.forename: Mikako
authors.initials: M
authors.lastname: Hashimoto
authors.forename: Takumi
authors.initials: T
authors.lastname: Adachi
authors.forename: Takako
authors.initials: T
authors.lastname: Matsushita
authors.forename: Hidetoshi
authors.initials: H
authors.lastname: Okuyama
authors.forename: Kiyohito
authors.initials: K
authors.lastname: Yoshida
mesh_terms: D000693:Anaerobiosis; D005227:Fatty Acids / Q000737:chemistry / Q000378:metabolism*; D045302:Geobacter / Q000737:chemistry* / Q000254:growth & development / Q000378:metabolism*; D006838:Hydrocarbons / Q000737:chemistry / Q000378:metabolism*
chemical_list: D005227:Fatty Acids; D006838:Hydrocarbons