title: In-plane microvortices micromixer-based AC electrothermal for testing drug induced death of tumor cells.
abstract: Herein, we first describe a perfusion chip integrated with an AC electrothermal (ACET) micromixer to supply a uniform drug concentration to tumor cells. The in-plane fluid microvortices for mixing were generated by six pairs of reconstructed novel ACET asymmetric electrodes. To enhance the mixing efficiency, the novel ACET electrodes with rotating angles of 0°, 30°, and 60° were investigated. The asymmetric electrodes with a rotating angle of 60° exhibited the highest mixing efficiency by both simulated and experimental results. The length of the mixing area is 7 mm, and the mixing efficiency is 89.12% (approximate complete mixing) at a voltage of 3 V and a frequency of 500 kHz. The applicability of our micromixer with electrodes rotating at 60° was demonstrated by the drug (tamoxifen) test of human breast cancer cells (MCF-7) for five days, which implies that our ACET in-plane microvortices micromixer has great potential for the application of drug induced rapid death of tumor cells and mixing of biomaterials in organs-on-a-chip systems.
authors.forename: Qi
authors.initials: Q
authors.lastname: Lang
authors.forename: Yukun
authors.initials: Y
authors.lastname: Ren
authors.affiliation: Interdisciplinary division of Biomedical Engineering, the Hong King Polytechnique University , Hunghom, Hong Kong, China.
authors.forename: Divia
authors.identifier: 0000-0002-9857-1033
authors.initials: D
authors.lastname: Hobson
authors.affiliation: School of Mechatronics Engineering, Harbin Institute of Technology , Harbin 150001, China.
authors.forename: Ye
authors.initials: Y
authors.lastname: Tao
authors.affiliation: School of Mechatronics Engineering, Harbin Institute of Technology , Harbin 150001, China.
authors.forename: Likai
authors.initials: L
authors.lastname: Hou
authors.affiliation: School of Mechatronics Engineering, Harbin Institute of Technology , Harbin 150001, China.
authors.forename: Yankai
authors.initials: Y
authors.lastname: Jia
authors.affiliation: School of Mechatronics Engineering, Harbin Institute of Technology , Harbin 150001, China.
authors.forename: Qingming
authors.initials: Q
authors.lastname: Hu
authors.affiliation: School of Mechatronics Engineering, Harbin Institute of Technology , Harbin 150001, China.
authors.forename: Jiangwei
authors.initials: J
authors.lastname: Liu
authors.affiliation: Interdisciplinary division of Biomedical Engineering, the Hong King Polytechnique University , Hunghom, Hong Kong, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Zhao
authors.forename: Hongyuan
authors.initials: H
authors.lastname: Jiang