title: The Effects of Hyperbaric Oxygen Treatment on Total Antioxidant Capacity and Prolidase Activity after Bile Duct Ligation in Rats.
abstract: BACKGROUND
  Hyperbaric oxygen (HBO) therapy may improve cholestasis, increase hepatic regeneration, and decrease oxidative stress in liver. In this study, we aimed to investigate the effects of HBO therapy on hepatic oxidative stress parameters, such as total thiol groups (T-SH), protein carbonyl (PCO), and total antioxidant capacity (TAC) as well as the predictive value of the noninvasive biochemical marker, sialic acid (SA), and prolidase activity in bile duct ligation (BDL)-induced oxidative damage and fibrosis in rats.
  
  
  METHODS
  We divided 32 adult male Sprague Dawley rats into four groups: sham, sham + HBO, BDL, and BDL + HBO; each group contained eight animals. We placed the sham + HBO and BDL + HBO groups in an experimental hyperbaric chamber, in which we administered pure oxygen at 2.5 atmospheres for 90 min on 14 consecutive days.
  
  
  RESULTS
  The application of BDL significantly increased PCO levels and prolidase activity, and decreased T-SH and TAC levels. HBO significantly decreased PCO levels and prolidase activity and increased T-SH and TAC levels in the liver tissues. There was no significant difference in sialic acid levels between any groups.
  
  
  CONCLUSIONS
  These results indicate that HBO therapy has hepatoprotective effects on BDL-induced injury by decreasing PCO and prolidase activity and increasing antioxidant activities. We therefore suggest that HBO therapy may be useful after BDL-induced injury.
authors.affiliation: a Department of Biochemistry , Cerrahpasa Faculty of Medicine, Istanbul University , Istanbul , Turkey.
authors.forename: Duygu
authors.initials: D
authors.lastname: Terzioglu
authors.affiliation: b Department of Nuclear Medicine , Cerrahpasa Faculty of Medicine, Istanbul University , Istanbul Turkey.
authors.forename: Lebriz
authors.initials: L
authors.lastname: Uslu
authors.affiliation: c Department of Physiology , Cerrahpasa Faculty of Medicine, Istanbul University , Istanbul , Turkey.
authors.forename: Gonul
authors.initials: G
authors.lastname: Simsek
authors.affiliation: a Department of Biochemistry , Cerrahpasa Faculty of Medicine, Istanbul University , Istanbul , Turkey.
authors.forename: Pinar
authors.initials: P
authors.lastname: Atukeren
authors.affiliation: a Department of Biochemistry , Cerrahpasa Faculty of Medicine, Istanbul University , Istanbul , Turkey.
authors.forename: Hayriye
authors.initials: H
authors.lastname: Erman
authors.affiliation: a Department of Biochemistry , Cerrahpasa Faculty of Medicine, Istanbul University , Istanbul , Turkey.
authors.forename: Remise
authors.initials: R
authors.lastname: Gelisgen
authors.affiliation: d Department of Pediatric Surgery , Faculty of Medicine, Trakya University , Edirne , Turkey.
authors.forename: Suleyman
authors.initials: S
authors.lastname: Ayvaz
authors.affiliation: d Department of Pediatric Surgery , Faculty of Medicine, Trakya University , Edirne , Turkey.
authors.forename: Burhan
authors.initials: B
authors.lastname: Aksu
authors.affiliation: a Department of Biochemistry , Cerrahpasa Faculty of Medicine, Istanbul University , Istanbul , Turkey.
authors.forename: Hafize
authors.initials: H
authors.lastname: Uzun
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D015415:Biomarkers / Q000032:analysis; D002779:Cholestasis / Q000209:etiology / Q000473:pathology / Q000628:therapy*; D003135:Common Bile Duct / Q000601:surgery; D004150:Dipeptidases / Q000097:blood / Q000378:metabolism*; D004195:Disease Models, Animal; D006801:Humans; D006931:Hyperbaric Oxygenation; D008026:Ligation; D008099:Liver / Q000378:metabolism / Q000473:pathology*; D008297:Male; D019158:N-Acetylneuraminic Acid / Q000032:analysis; D018384:Oxidative Stress; D010100:Oxygen / Q000627:therapeutic use; D011237:Predictive Value of Tests; D051381:Rats; D017207:Rats, Sprague-Dawley; D013053:Spectrophotometry
chemical_list: D000975:Antioxidants; D015415:Biomarkers; D004150:Dipeptidases; C022449:proline dipeptidase; D019158:N-Acetylneuraminic Acid; D010100:Oxygen
keywords: ferric-reducing antioxidant power; hyperbaric oxygen therapy; liver fibrosis; prolidase activity; protein carbonyl groups; sialic acid