title: Distinct and Shared Determinants of Cardiomyocyte Contractility in Multi-Lineage Competent Ethnically Diverse Human iPSCs.
abstract: The realization of personalized medicine through human induced pluripotent stem cell (iPSC) technology can be advanced by transcriptomics, epigenomics, and bioinformatics that inform on genetic pathways directing tissue development and function. When possible, population diversity should be included in new studies as resources become available. Previously we derived replicate iPSC lines of African American, Hispanic-Latino and Asian self-designated ethnically diverse (ED) origins with normal karyotype, verified teratoma formation, pluripotency biomarkers, and tri-lineage in vitro commitment. Here we perform bioinformatics of RNA-Seq and ChIP-seq pluripotency data sets for two replicate Asian and Hispanic-Latino ED-iPSC lines that reveal differences in generation of contractile cardiomyocytes but similar and robust differentiation to multiple neural, pancreatic, and smooth muscle cell types. We identify shared and distinct genes and contributing pathways in the replicate ED-iPSC lines to enhance our ability to understand how reprogramming to iPSC impacts genes and pathways contributing to cardiomyocyte contractility potential.
authors.affiliation: State University of New York Polytechnic Institute, Colleges of Nanoscale Science and Engineering (SUNY Poly, CNSE), Nanobioscience, Albany, NY, 12203, USA.
authors.forename: Martin L
authors.initials: ML
authors.lastname: Tomov
authors.affiliation: State University of New York Polytechnic Institute, Colleges of Nanoscale Science and Engineering (SUNY Poly, CNSE), Nanobioscience, Albany, NY, 12203, USA.
authors.forename: Zachary T
authors.initials: ZT
authors.lastname: Olmsted
authors.affiliation: University of Nebraska-Lincoln, Electrical and Computer Engineering, Lincoln, NE, 68588, USA.
authors.forename: Haluk
authors.initials: H
authors.lastname: Dogan
authors.affiliation: Istanbul University Faculty of Medicine, Institute of Experimental Medicine Research (DETAE), Department of Genetics, Fatih, Istanbul, 34393, Turkey.
authors.forename: Eda
authors.initials: E
authors.lastname: Gongorurler
authors.affiliation: State University of New York Buffalo (SUNY Buffalo), Department of Biochemistry, Center of Excellence in Bioinformatics, Buffalo, NY, 14203, USA.
authors.forename: Maria
authors.initials: M
authors.lastname: Tsompana
authors.affiliation: University of Nebraska-Lincoln, Electrical and Computer Engineering, Lincoln, NE, 68588, USA.
authors.forename: Hasan H
authors.initials: HH
authors.lastname: Otu
authors.affiliation: State University of New York Buffalo (SUNY Buffalo), Department of Biochemistry, Center of Excellence in Bioinformatics, Buffalo, NY, 14203, USA.
authors.forename: Michael
authors.initials: M
authors.lastname: Buck
authors.affiliation: Eulji Medical Center, Eulji University, Department of Laboratory Medicine, Seoul, Korea.|Michigan State University, Departments of Animal Science and Physiology, East Lansing, MI, 48824, USA.
authors.forename: Eun-Ah
authors.initials: EA
authors.lastname: Chang
authors.affiliation: Michigan State University, Departments of Animal Science and Physiology, East Lansing, MI, 48824, USA.|LARCEL, Laboratorio Andaluz de Reprogramación Celular, BIONAND, Andalucia, 29590, Spain.
authors.forename: Jose
authors.initials: J
authors.lastname: Cibelli
authors.affiliation: State University of New York Polytechnic Institute, Colleges of Nanoscale Science and Engineering (SUNY Poly, CNSE), Nanobioscience, Albany, NY, 12203, USA.
authors.forename: Janet L
authors.initials: JL
authors.lastname: Paluh
mesh_terms: D015415:Biomarkers; D002454:Cell Differentiation / Q000235:genetics*; D005006:Ethnicity / Q000235:genetics; D018507:Gene Expression Regulation, Developmental; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000166:cytology*; D032383:Myocytes, Cardiac / Q000166:cytology; D057285:Precision Medicine; D059467:Transcriptome / Q000235:genetics*
chemical_list: D015415:Biomarkers