title: Role for PADI6 in securing the mRNA-MSY2 complex to the oocyte cytoplasmic lattices.
abstract: The oocyte cytoplasmic lattices (CPLs) have long been predicted to function as a storage form for the maternal contribution of ribosomes to the early embryo. Our previous studies have demonstrated that ribosomal component S6 is stored in the oocyte CPLs and peptidylarginine deiminase 6 (PADI6) is critical for CPLs formation. Additionally, we found that depletion of PADI6 reduced de novo protein synthesis prior to the maternal-to-embryonic transition, therefore causing embryos to arrest at the 2-cell stage. Here, we present evidence further supporting the association of ribosomes with the CPLs by demonstrating that rRNAs are dramatically decreased in Padi6 KO oocytes. We also show that the abundance and localization of mRNAs is affected upon PADI6 depletion, suggesting that mRNAs are very possibly associated with CPLs. Consistent with this observation, the amount of the major RNA binding protein, MSY2, that is associated with the insoluble fraction of the oocytes after Triton X-100 extraction is also markedly decreased in the Padi6 KO oocytes. Furthermore, treatment of the oocytes with RNase A followed by Triton X-100 extraction severely impairs the localization of PADI6 and MSY2 in oocytes. These results indicate that mRNAs, possibly in a complex with MSY2 and PADI6, are bound in the CPLs and may play a role in securing the mRNA-MSY2 complex to the CPLs.
authors.affiliation: a Key Laboratory of Pathogen Biology of Jiangsu Province , Nanjing Medical University , Nanjing , China.|b Department of Microbiology , Nanjing Medical University , Nanjing , China.
authors.forename: Xiaoqiu
authors.initials: X
authors.lastname: Liu
authors.affiliation: c Baker Institute for Animal Health, College of Veterinary Medicine, Cornell University , Ithaca , NY USA.
authors.forename: Eric
authors.initials: E
authors.lastname: Morency
authors.affiliation: d State Key Laboratory of Reproductive Medicine , Nanjing Medical University , Nanjing , China.
authors.forename: Tingting
authors.initials: T
authors.lastname: Li
authors.affiliation: d State Key Laboratory of Reproductive Medicine , Nanjing Medical University , Nanjing , China.
authors.forename: Hao
authors.initials: H
authors.lastname: Qin
authors.affiliation: d State Key Laboratory of Reproductive Medicine , Nanjing Medical University , Nanjing , China.
authors.forename: Xiaoqian
authors.initials: X
authors.lastname: Zhang
authors.affiliation: d State Key Laboratory of Reproductive Medicine , Nanjing Medical University , Nanjing , China.
authors.forename: Xuesen
authors.initials: X
authors.lastname: Zhang
authors.affiliation: c Baker Institute for Animal Health, College of Veterinary Medicine, Cornell University , Ithaca , NY USA.
authors.forename: Scott
authors.initials: S
authors.lastname: Coonrod
mesh_terms: D000818:Animals; D003593:Cytoplasm / Q000378:metabolism*; D005260:Female; D006867:Hydrolases / Q000378:metabolism*; D018345:Mice, Knockout; D009865:Oocytes / Q000378:metabolism*; D000080065:Protein-Arginine Deiminase Type 6; D000076342:Protein-Arginine Deiminases; D034443:RNA Transport; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012335:RNA, Ribosomal / Q000378:metabolism; D016601:RNA-Binding Proteins / Q000378:metabolism*; D012995:Solubility
chemical_list: D006867:Hydrolases; D000080065:Protein-Arginine Deiminase Type 6; D000076342:Protein-Arginine Deiminases; D012333:RNA, Messenger; D012335:RNA, Ribosomal; D016601:RNA-Binding Proteins; C116047:Ybx2 protein, mouse; C523190:PADI6 protein, mouse
keywords: MSY2; PADI6; cytoplasmic lattice; oocyte; ribosomal storage