title: Hybrid Synthetic Receptors on MOSFET Devices for Detection of Prostate Specific Antigen in Human Plasma.
abstract: The study reports the use of extended gate field-effect transistors (FET) for the label-free and sensitive detection of prostate cancer (PCa) biomarkers in human plasma. The approach integrates for the first time hybrid synthetic receptors comprising of highly selective aptamer-lined pockets (apta-MIP) with FETs for sensitive detection of prostate specific antigen (PSA) at clinically relevant concentrations. The hybrid synthetic receptors were constructed by immobilizing an aptamer-PSA complex on gold and subjecting it to 13 cycles of dopamine electropolymerization. The polymerization resulted in the creation of highly selective polymeric cavities that retained the ability to recognize PSA post removal of the protein. The hybrid synthetic receptors were subsequently used in an extended gate FET setup for electrochemical detection of PSA. The sensor was reported to have a limit of detection of 0.1 pg/mL with a linear detection range from 0.1 pg/mL to 1 ng/mL PSA. Detection of 1-10 pg/mL PSA was also achieved in diluted human plasma. The present apta-MIP sensor developed in conjunction with FET devices demonstrates the potential for clinical application of synthetic hybrid receptors for the detection of clinically relevant biomarkers in complex samples.
authors.affiliation: School of Pharmacy and Pharmaceutical Sciences, Cardiff University , Cardiff CF24 3AJ, United Kingdom.
authors.forename: Vibha K
authors.initials: VK
authors.lastname: Tamboli
authors.affiliation: Department of Electronic & Electrical Engineering, University of Bath , Bath BA2 7AY, United Kingdom.
authors.forename: Nikhil
authors.initials: N
authors.lastname: Bhalla
authors.affiliation: Department of Electronic & Electrical Engineering, University of Bath , Bath BA2 7AY, United Kingdom.
authors.forename: Pawan
authors.initials: P
authors.lastname: Jolly
authors.affiliation: Department of Mechanical Engineering, University of Bath , Bath BA2 7AY, United Kingdom.
authors.forename: Chris R
authors.initials: CR
authors.lastname: Bowen
authors.affiliation: Department of Electronic & Electrical Engineering, University of Bath , Bath BA2 7AY, United Kingdom.
authors.forename: John T
authors.initials: JT
authors.lastname: Taylor
authors.affiliation: School of Pharmacy and Pharmaceutical Sciences, Cardiff University , Cardiff CF24 3AJ, United Kingdom.
authors.forename: Jenna L
authors.initials: JL
authors.lastname: Bowen
authors.affiliation: School of Pharmacy and Pharmaceutical Sciences, Cardiff University , Cardiff CF24 3AJ, United Kingdom.
authors.forename: Chris J
authors.initials: CJ
authors.lastname: Allender
authors.affiliation: Department of Electronic & Electrical Engineering, University of Bath , Bath BA2 7AY, United Kingdom.
authors.forename: Pedro
authors.initials: P
authors.lastname: Estrela
mesh_terms: D052157:Aptamers, Nucleotide / Q000097:blood; D015374:Biosensing Techniques; D006046:Gold / Q000737:chemistry*; D006801:Humans; D008297:Male; D010087:Oxides / Q000737:chemistry*; D017430:Prostate-Specific Antigen / Q000097:blood*; D011471:Prostatic Neoplasms / Q000097:blood*; D062165:Receptors, Artificial / Q000138:chemical synthesis / Q000737:chemistry*; D012666:Semiconductors; D014173:Transistors, Electronic
chemical_list: D052157:Aptamers, Nucleotide; D010087:Oxides; D062165:Receptors, Artificial; D006046:Gold; D017430:Prostate-Specific Antigen