title: Protective activity of Hertia cheirifolia extracts against DNA damage, lipid peroxidation and protein oxidation.
abstract: BACKGROUND
  Hertia cheirifolia L. (Asteraceae), a perennial shrub widely distributed in Northern Africa, is traditionally used to treat inflammatory disorders.
  
  
  OBJECTIVE
  The protective effect of methanol (Met E) and aqueous (Aq E) extracts of Hertia cheirifolia against DNA, lipid and protein oxidation was investigated.
  
  
  METHODS
  Different concentrations (50-1000 μg/mL) of Hertia cheirifolia aerial part extracts were examined against DNA, lipid and protein oxidation induced by H2O2+ UV, FeSO4, and Fe3+/H2O2-ascorbic acid, respectively. The DPPH•, metal ion chelating, reducing power and β-carotene bleaching tests were conducted.
  
  
  RESULTS
  Both extracts were rich in polyphenols, flavonoids and tannins, and were able to scavenge DPPH•with IC50values of 138 and 197 μg/mL, respectively. At 300 μg/mL, Aq E exerted stronger chelating effect (99%) than Met E (69%). However, Met E reducing power (IC50=61 μg/mL) was more than that of Aq E (IC50=193 μg/mL). Both extracts protected from β-carotene bleaching by 74% and 94%, respectively, and inhibited linoleic acid peroxidation. The inhibitory activity of Aq E extract (64%) was twice more than that of Met E (32%). Interestingly, both extracts protected DNA against the cleavage by about 96-98%. At 1 mg/mL, Met E and Aq E restored protein band intensity by 94-99%.
  
  
  CONCLUSIONS
  Hertia cheirifolia exhibits potent antioxidant activity and protects biomolecules against oxidative damage; hence, it may serve as potential source of natural antioxidant for pharmaceutical applications and food preservation. This is the first report on the protective activity of this plant against biomolecule oxidation.
authors.affiliation: a Laboratory of Applied Biochemistry, Faculty of SNV , University Ferhat Abbas , Sétif , Algeria.
authors.forename: Seoussen
authors.initials: S
authors.lastname: Kada
authors.affiliation: a Laboratory of Applied Biochemistry, Faculty of SNV , University Ferhat Abbas , Sétif , Algeria.
authors.forename: Hamama
authors.initials: H
authors.lastname: Bouriche
authors.affiliation: a Laboratory of Applied Biochemistry, Faculty of SNV , University Ferhat Abbas , Sétif , Algeria.
authors.forename: Abderrahmane
authors.initials: A
authors.lastname: Senator
authors.affiliation: b Department of Chemistry, Faculty of Science , Cankırı Karatekin University , Cankırı , Turkey.
authors.forename: Ibrahim
authors.initials: I
authors.lastname: Demirtaş
authors.affiliation: c Department of Chemistry, Faculty of Science and Arts , Ondokuz Mayis University , Samsun , Turkey.
authors.forename: Tevfik
authors.initials: T
authors.lastname: Özen
authors.affiliation: d Department of Chemistry, Faculty of Science and Arts , University of Dicle , Diyarbakır , Turkey.
authors.forename: Bircan
authors.initials: B
authors.lastname: Çeken Toptanci
authors.affiliation: d Department of Chemistry, Faculty of Science and Arts , University of Dicle , Diyarbakır , Turkey.
authors.forename: Göksel
authors.initials: G
authors.lastname: Kızıl
authors.affiliation: d Department of Chemistry, Faculty of Science and Arts , University of Dicle , Diyarbakır , Turkey.
authors.forename: Murat
authors.initials: M
authors.lastname: Kızıl
mesh_terms: D000975:Antioxidants / Q000302:isolation & purification / Q000494:pharmacology*; D019659:Asteraceae / Q000737:chemistry*; D001713:Biphenyl Compounds / Q000737:chemistry; D002712:Chlorides / Q000737:chemistry; D004249:DNA Damage / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D005290:Ferric Compounds / Q000737:chemistry; D007502:Iron Chelating Agents / Q000302:isolation & purification / Q000494:pharmacology; D015227:Lipid Peroxidation / Q000187:drug effects*; D000432:Methanol / Q000737:chemistry; D010084:Oxidation-Reduction; D018384:Oxidative Stress / Q000187:drug effects*; D008517:Phytotherapy; D010851:Picrates / Q000737:chemistry; D035261:Plant Components, Aerial; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology*; D010946:Plants, Medicinal; D059808:Polyphenols / Q000302:isolation & purification / Q000494:pharmacology; D050050:Protein Carbonylation / Q000187:drug effects*; D012997:Solvents / Q000737:chemistry; D013634:Tannins / Q000302:isolation & purification / Q000494:pharmacology; D014867:Water / Q000737:chemistry; D019207:beta Carotene / Q000737:chemistry
chemical_list: D000975:Antioxidants; D001713:Biphenyl Compounds; D002712:Chlorides; D005290:Ferric Compounds; D007502:Iron Chelating Agents; D010851:Picrates; D010936:Plant Extracts; D059808:Polyphenols; D012997:Solvents; D013634:Tannins; D019207:beta Carotene; D014867:Water; C004931:1,1-diphenyl-2-picrylhydrazyl; C024555:ferric chloride; D000432:Methanol
keywords: Antioxidant activity; biomolecule lesions; medicinal plant; oxidative stress; polyphenols