title: Cytokine Attenuation and Free Radical Scavenging Activity of a New Flavanone7,4'-Dihydroxy-3″,3″-Dimethyl -(5,6-Pyrano-2″-One)- 8- (3‴,3‴-Dimethyl Allyl)- Isolated from Mallotus philippensis: Possible Mechanism for Its Anti-Inflammatory Activity.
abstract: Mallotus philippensis L.(MP) commonly known as Kamala tree in Hindi,is a small to medium-sized monoecious tree.The objective of the study was to evaluate the anti-inflammatory activity of MPand a new flavanoneisolated from it by using in vivo models of inflammation.Albino wistar rats of either sex weighing 150-200g were used. Seven groups were made (n = 6), namely normal control group (normal saline, 1 ml/kg), standard control group (acetylsalicylic acid, 100 mg/kg), methanol crude extract (300 and 500 mg/kg), ethylacetate fraction (300 and 500 mg/kg) and active compound 4 (new flavanone, 50 mg/kg). The anti-inflammatory activity was studied using carrageenan induced paw edema method and cotton pellet granuloma method. Levels of cytokines (TNF-α, IL-1and IL-6) and activity of antioxidant enzymeslike catalase and glutathione peroxidase were estimated. It was found that the methanol extract, ethylacetate fraction and Flavanonedemonstrated significant reduction in paw edema in carrageenan induced paw edema method as compared to control. They also diminished the serum TNF-α, IL-6 and IL-1 levels. Significantly attenuated the malondialdehyde levels and increased the activities of catalase and glutathione peroxidase in paw tissue. Similarly there was asignificant decrease in granuloma formation in cotton pellet induced granuloma method. In conclusion, MP extracts and the newflavanonepossess anti-inflammatory activity and this might be due to the inhibition of various cytokines and increased free radical scavenging activity.
authors.affiliation: Department of Pharmacology, Jawaharlal NehruMedical College, Aligarh Muslim University, Aligarh, India.
authors.forename: Waseeem
authors.initials: W
authors.lastname: Rizvi
authors.affiliation: Department of Pharmacology, Jawaharlal NehruMedical College, Aligarh Muslim University, Aligarh, India.
authors.forename: Mohd
authors.initials: M
authors.lastname: Fayazuddin
authors.affiliation: Chemical Research Unit Department of research in Unani Medicine Aligarh Muslim University, Aligarh, India.
authors.forename: Ompal
authors.initials: O
authors.lastname: Singh
authors.affiliation: Department of Pharmacology, Jawaharlal NehruMedical College, Aligarh Muslim University, Aligarh, India.
authors.forename: Syed Shariq
authors.initials: SS
authors.lastname: Naeem
authors.affiliation: Department of Biochemistry, Jawaharlal NehruMedical College, Aligarh Muslim University, Aligarh, India.
authors.forename: Shagufta
authors.initials: S
authors.lastname: Moin
authors.affiliation: Department of Pathology, Jawaharlal NehruMedical College, Aligarh Muslim University, Aligarh, India.
authors.forename: Kafil
authors.initials: K
authors.lastname: Akhtar
authors.affiliation: Department of Pharmacology, Jawaharlal NehruMedical College, Aligarh Muslim University, Aligarh, India.
authors.forename: Anil
authors.initials: A
authors.lastname: Kumar
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000737:chemistry / Q000494:pharmacology; D002351:Carrageenan / Q000009:adverse effects; D002374:Catalase / Q000037:antagonists & inhibitors / Q000378:metabolism; D016207:Cytokines / Q000097:blood / Q000378:metabolism*; D004487:Edema / Q000188:drug therapy / Q000209:etiology / Q000473:pathology; D044950:Flavanones / Q000737:chemistry / Q000494:pharmacology*; D016166:Free Radical Scavengers / Q000737:chemistry / Q000494:pharmacology*; D005979:Glutathione Peroxidase / Q000037:antagonists & inhibitors / Q000378:metabolism; D006099:Granuloma / Q000188:drug therapy / Q000209:etiology / Q000473:pathology; D007249:Inflammation / Q000188:drug therapy / Q000209:etiology / Q000473:pathology; D015227:Lipid Peroxidation / Q000187:drug effects; D036461:Mallotus Plant / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D051381:Rats
chemical_list: D000893:Anti-Inflammatory Agents; D016207:Cytokines; D044950:Flavanones; D016166:Free Radical Scavengers; D010936:Plant Extracts; D002351:Carrageenan; D002374:Catalase; D005979:Glutathione Peroxidase