title: Inhibitory effect of Sophora subprosrate polysaccharide on mitochondria oxidative stress induced by PCV-2 infection in RAW264.7 cells.
abstract: In the present study, the inhibitory effect of Sophora subprosrate polysaccharide (SSP) on PCV-2-induced mitochondrial respiratory burst in RAW264.7 cells was first investigated. The findings suggested that SOD activity and the anti-superoxide anion radical activity of the RAW264.7 cells were significantly decreased after PCV-2 infection, and MnSOD mRNA levels were significantly decreased, while NOX2 mRNA levels and protein expression were increased. Meanwhile, the O2•-levels and mitochondrial membrane potentials were significantly increased. After treatment with SSP, significant increases in the activities of SOD, anti-superoxide anion radical activities, and MnSOD mRNA levels in the PCV-2 infected cells were observed. Meanwhile, significant increases in NOX2 mRNA levels and protein expression, O2•-levels and mitochondrial membrane potentials were also observed. The results showed that PCV2 infection resulted in the mitochondria oxidative stress of RAW264.7 cells as indicated by an increasing mitochondrial membrane potential, which was then inhibited by SSP. It was concluded that RAW264.7 cells treated with SSP could suffer from mitochondrial damage, which may be mediated by the inhibition of the mitochondrial membrane potential.
authors.affiliation: College of Animal Science and Technology, Guangxi University, Nanning 530005, PR China.
authors.forename: Zi-Jie
authors.initials: ZJ
authors.lastname: Su
authors.affiliation: College of Animal Science and Technology, Guangxi University, Nanning 530005, PR China.
authors.forename: Jian
authors.initials: J
authors.lastname: Yang
authors.affiliation: College of Animal Science and Technology, Guangxi University, Nanning 530005, PR China.
authors.forename: Wen-Juan
authors.initials: WJ
authors.lastname: Luo
authors.affiliation: College of Animal Science and Technology, Guangxi University, Nanning 530005, PR China.
authors.forename: Ying-Yi
authors.initials: YY
authors.lastname: Wei
authors.affiliation: Veterinary Department of Rongchang Campuses, Southwest University, Rongchang, Chongqing 402460, PR China.
authors.forename: Xue-Hong
authors.initials: XH
authors.lastname: Shuai
authors.affiliation: College of Animal Science and Technology, Guangxi University, Nanning 530005, PR China. Electronic address: tingjunhu@126.com.
authors.forename: Ting-Jun
authors.initials: TJ
authors.lastname: Hu
mesh_terms: D000818:Animals; D017925:Circovirus / Q000502:physiology*; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D008562:Membrane Glycoproteins / Q000235:genetics / Q000378:metabolism; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D051379:Mice; D008928:Mitochondria / Q000187:drug effects* / Q000378:metabolism*; D000074662:NADPH Oxidase 2; D019255:NADPH Oxidases / Q000235:genetics / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects*; D000067996:RAW 264.7 Cells; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D029909:Sophora / Q000737:chemistry*; D013482:Superoxide Dismutase / Q000378:metabolism; D013481:Superoxides / Q000378:metabolism
chemical_list: D008562:Membrane Glycoproteins; D012333:RNA, Messenger; D013481:Superoxides; D013482:Superoxide Dismutase; C488842:Cybb protein, mouse; D000074662:NADPH Oxidase 2; D019255:NADPH Oxidases
keywords: Mitochondria oxidative stress; Porcine circovirus type 2 (PCV2); Sophora subprosrate polysaccharide (SSP)