title: Shedding of porcine circovirus type 1 DNA and rotavirus RNA by infants vaccinated with Rotarix®.
abstract: Thirty-three infants aged ∼2 months had serial stool samples collected after receipt of Rotarix® vaccine dose 1, and were assessed for shedding of porcine circovirus type 1 DNA and Rotavirus group A RNA by molecular methods. We did not find strong evidence that porcine circovirus type 1 replication occurred. Porcine circovirus type 1 genome with the same sequence as that in Rotarix® was detected in a few infants as late as day ≥ 13; while this timing could suggest there may have been replication and not just transient passage through the gastrointestinal tract, the lack of increase in copy number in any infant supports transient passage and there are inherent limitations to the results. We found that 21% of infants did not shed Rotarix® RVA RNA beyond the day 3 sample, which may suggest lack of vaccine virus replication. Of the infants in whom Rotarix RVA RNA shedding continued, peak copy numbers were reached on days 3-5 for ∼40%, and after day 5 in ∼60%, and shedding can be prolonged (≥ 45 days).
authors.affiliation: a Division of Viral Diseases , National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention , Atlanta , GA , USA.
authors.forename: Slavica
authors.initials: S
authors.lastname: Mijatovic-Rustempasic
authors.affiliation: b Morehouse School of Medicine, Clinical Research Center , Departments of Microbiology/Biochemistry/Immunology and Pediatrics , Atlanta , GA.
authors.forename: Lilly Cheng
authors.initials: LC
authors.lastname: Immergluck
authors.affiliation: b Morehouse School of Medicine, Clinical Research Center , Departments of Microbiology/Biochemistry/Immunology and Pediatrics , Atlanta , GA.
authors.forename: Trisha Chan
authors.initials: TC
authors.lastname: Parker
authors.affiliation: b Morehouse School of Medicine, Clinical Research Center , Departments of Microbiology/Biochemistry/Immunology and Pediatrics , Atlanta , GA.
authors.forename: Elham
authors.initials: E
authors.lastname: Laghaie
authors.affiliation: b Morehouse School of Medicine, Clinical Research Center , Departments of Microbiology/Biochemistry/Immunology and Pediatrics , Atlanta , GA.
authors.forename: Anaam
authors.initials: A
authors.lastname: Mohammed
authors.affiliation: c Emory University , Department of Pediatrics , Atlanta , GA.
authors.forename: Terri
authors.initials: T
authors.lastname: McFadden
authors.affiliation: a Division of Viral Diseases , National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention , Atlanta , GA , USA.
authors.forename: Umesh D
authors.initials: UD
authors.lastname: Parashar
authors.affiliation: a Division of Viral Diseases , National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention , Atlanta , GA , USA.
authors.forename: Michael D
authors.initials: MD
authors.lastname: Bowen
authors.affiliation: a Division of Viral Diseases , National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention , Atlanta , GA , USA.
authors.forename: Margaret M
authors.initials: MM
authors.lastname: Cortese
mesh_terms: D017925:Circovirus / Q000235:genetics / Q000302:isolation & purification*; D004279:DNA, Viral / Q000235:genetics / Q000302:isolation & purification*; D005243:Feces / Q000821:virology*; D005260:Female; D006801:Humans; D007223:Infant; D008297:Male; D012367:RNA, Viral / Q000235:genetics / Q000302:isolation & purification*; D012401:Rotavirus / Q000235:genetics / Q000302:isolation & purification*; D022243:Rotavirus Vaccines / Q000008:administration & dosage*; D013997:Time Factors; D014613:Vaccines, Attenuated / Q000008:administration & dosage; D017201:Virus Shedding
chemical_list: D004279:DNA, Viral; C492457:RIX4414 vaccine; D012367:RNA, Viral; D022243:Rotavirus Vaccines; D014613:Vaccines, Attenuated
keywords: PCV-1; Porcine circivirus-1; Rotarix; Rotarix® PCV-1 DNA; Rotarix® RVA RNA; rotavirus; rotavirus group A; shedding