title: Effect of FK-506 (tacrolimus) therapy on bone healing of titanium implants: a histometric and biomechanical study in mice.
abstract: Immunosuppressive agents have been recognized as a factor affecting bone metabolism. The aim of the present study was to evaluate the influence of FK-506 administration on the healing of bone around titanium implants. Thirty-two male mice were randomly allocated to two groups: the FK-506 group (n = 16 mice), which received subcutaneous administration of FK-506 (1 mg kg-1d-1); and the control group (n = 16 mice), which received saline solution by the same route of administration. After 2 wk, one titanium implant with sandblasted/acid-etched surface was placed in the femur. The therapy continued until the mice were killed 2 and 4 wk after surgery. The femurs with implants were evaluated by biomechanical testing and histologic analysis. The bone-implant contact (BIC) and bone volume (BV/TV) within a 100-μm-wide circumferential zone lateral to the implant surface were histomorphometrically analyzed. Compared with the control group, the FK-506 group showed significantly lower BIC and BV/TV at both 2 and 4 wk. Biomechanical tests showed that FK-506 significantly impaired the strength of bone-implant integration at both 2 and 4 wk postoperatively. Our data indicate that immunosuppressive therapy with FK-506 negatively affects the fixation of titanium implants.
authors.affiliation: State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, China.|Dental Implant Center, West China Hospital of Stomatology, Sichuan University, Chengdu, China.
authors.forename: Xiaofei
authors.initials: X
authors.lastname: Zheng
authors.affiliation: State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, China.|Dental Implant Center, West China Hospital of Stomatology, Sichuan University, Chengdu, China.
authors.forename: Anchun
authors.initials: A
authors.lastname: Mo
authors.affiliation: State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, China.|Dental Implant Center, West China Hospital of Stomatology, Sichuan University, Chengdu, China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, China.|Dental Implant Center, West China Hospital of Stomatology, Sichuan University, Chengdu, China.
authors.forename: Yuchen
authors.initials: Y
authors.lastname: Guo
authors.affiliation: State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, China.|Dental Implant Center, West China Hospital of Stomatology, Sichuan University, Chengdu, China.
authors.forename: Yunshu
authors.initials: Y
authors.lastname: Wu
authors.affiliation: State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, China.|Dental Implant Center, West China Hospital of Stomatology, Sichuan University, Chengdu, China.
authors.forename: Quan
authors.initials: Q
authors.lastname: Yuan
mesh_terms: D000818:Animals; D001696:Biomechanical Phenomena; D000069343:Bone-Implant Interface; D015921:Dental Implants; D005269:Femur / Q000601:surgery*; D020007:Implants, Experimental; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D016348:Osseointegration / Q000187:drug effects*; D013499:Surface Properties; D016559:Tacrolimus / Q000494:pharmacology*; D014025:Titanium; D014945:Wound Healing / Q000187:drug effects*
chemical_list: D015921:Dental Implants; D014025:Titanium; D016559:Tacrolimus
keywords: FK-506; bone healing; osseointegration; titanium implant