title: High-fat Diet Enhances Mammary Tumorigenesis and Pulmonary Metastasis and Alters Inflammatory and Angiogenic Profiles in MMTV-PyMT Mice.
abstract: The MMTV-PyMT transgenic mouse model is commonly used to study luminal B subtype, which has a lower prevalence but a worse prognosis than luminal A subtype among patients with breast cancer. The objective of the present study was to determine whether an obesogenic, high-fat diet enhances primary tumorigenesis and pulmonary metastasis in female MMTV-PyMT mice. The high-fat diet slightly but significantly increased caloric intake and body fat mass compared to the AIN93G diet. The high-fat diet significantly increased primary mammary tumor progression by 59%, primary tumor weight by 60%, and the number of lung metastases by 147%. Compared to the AIN93G diet, the high-fat diet significantly increased the abundance of proinflammatory cytokines (e.g. leptin, monocyte chemotactic protein-1, plasminogen activator inhibitor-1, resistin, and tumor necrosis factor-α) and angiogenic factors (e.g. hepatocyte growth factor, tissue inhibitor of metalloproteinase inhibitor-1, and vascular endothelial growth factor) in plasma and mammary tumors. We conclude that the obesogenic high-fat diet enhances primary tumorigenesis and metastasis in MMTV-PyMT mice. This enhancement may be the result of increased proinflammation and angiogenesis signaling.
authors.affiliation: U.S. Department of Agriculture, Agricultural Research Service, Grand Forks Human Nutrition Research Center, Grand Forks, ND, U.S.A.
authors.forename: Sneha
authors.initials: S
authors.lastname: Sundaram
authors.affiliation: U.S. Department of Agriculture, Agricultural Research Service, Grand Forks Human Nutrition Research Center, Grand Forks, ND, U.S.A. lin.yan@ars.usda.gov.
authors.forename: Lin
authors.initials: L
authors.lastname: Yan
mesh_terms: D054392:Adipokines / Q000378:metabolism; D000818:Animals; D001823:Body Composition; D001835:Body Weight; D059305:Diet, High-Fat; D002149:Energy Intake; D005260:Female; D008175:Lung Neoplasms / Q000098:blood supply / Q000378:metabolism / Q000556:secondary*; D008325:Mammary Neoplasms, Experimental / Q000098:blood supply / Q000378:metabolism / Q000473:pathology*; D051379:Mice; D009389:Neovascularization, Pathologic
chemical_list: D054392:Adipokines
keywords: MMTV-PyMT; Mammary tumor; high-fat diet; metastasis; mouse model