title: [Increased expression of IL-9 in peripheral blood and liver tissues of patients with primary biliary cirrhosis].
abstract: Objective To investigate the expression and clinical significance of interleukin 9 (IL-9) in patients with primary biliary cirrhosis (PBC). Methods The study enrolled 80 PBC patients and 100 healthy subjects. Levels of serum IL-9, interferon γ (IFN-γ), IL-6, tumor necrosis factor α (TNF-α), IL-10 and IL-17 were determined by cytometric beads array (CBA); level of IL-9 mRNA in peripheral blood mononuclear cells (PBMCs) was detected by quantitative real-time PCR; the expression and location of IL-9 in liver tissues were detected by immunohistochemistry. Results Serum levels of IL-9, TNF-α, IL-6, IL-17, IFN-γ and IL-10 significantly increased in PBC patients in comparison with healthy controls, and a positively correlation between IL-9 and IgG was observed in PBC patients. In addition, the expression of IL-9 mRNA in PBC groups increased. Immunohistochemical results suggested that positive cells appeared in the PBC liver tissue, mainly in the portal area. Conclusion Expression of IL-9 remarkably increases in peripheral blood and liver tissues in patients with PBC.
authors.affiliation: First Affiliated Hospital, Xinjiang Medical University, Key Laboratory of Major Diseases-State Key Laboratory Cultivation Base in Xinjiang, Urumqi 830011, China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Wang
authors.affiliation: First Affiliated Hospital, Xinjiang Medical University, Key Laboratory of Major Diseases-State Key Laboratory Cultivation Base in Xinjiang, Urumqi 830011, China.
authors.forename: Demei
authors.initials: D
authors.lastname: Luo
authors.affiliation: First Affiliated Hospital, Xinjiang Medical University, Key Laboratory of Major Diseases-State Key Laboratory Cultivation Base in Xinjiang, Urumqi 830011, China.
authors.forename: Xiaofeng
authors.initials: X
authors.lastname: Sun
authors.affiliation: First Affiliated Hospital, Xinjiang Medical University, Key Laboratory of Major Diseases-State Key Laboratory Cultivation Base in Xinjiang, Urumqi 830011, China.
authors.forename: Ning
authors.initials: N
authors.lastname: Feng
authors.affiliation: First Affiliated Hospital, Xinjiang Medical University, Key Laboratory of Major Diseases-State Key Laboratory Cultivation Base in Xinjiang, Urumqi 830011, China.
authors.forename: Huijun
authors.initials: H
authors.lastname: Li
authors.affiliation: First Affiliated Hospital, Xinjiang Medical University, Key Laboratory of Major Diseases-State Key Laboratory Cultivation Base in Xinjiang, Urumqi 830011, China.
authors.forename: Jinwei
authors.initials: J
authors.lastname: Hu
authors.affiliation: First Affiliated Hospital, Xinjiang Medical University, Key Laboratory of Major Diseases-State Key Laboratory Cultivation Base in Xinjiang, Urumqi 830011; School of Basic Medical Sciences, Xinjiang Medical University, Urumqi 830011, China.
authors.forename: Wenqiang
authors.initials: W
authors.lastname: Yang
authors.affiliation: First Affiliated Hospital, Xinjiang Medical University, Key Laboratory of Major Diseases-State Key Laboratory Cultivation Base in Xinjiang, Urumqi 830011; School of Basic Medical Sciences, Xinjiang Medical University, Urumqi 830011, China. *Corresponding author, E-mail: maxiumin1210@163.com.
authors.forename: Xiumin
authors.initials: X
authors.lastname: Ma
mesh_terms: D000328:Adult; D005260:Female; D006801:Humans; D007371:Interferon-gamma / Q000097:blood / Q000378:metabolism; D016753:Interleukin-10 / Q000097:blood / Q000378:metabolism; D015850:Interleukin-6 / Q000097:blood / Q000378:metabolism; D016906:Interleukin-9 / Q000097:blood* / Q000378:metabolism*; D007963:Leukocytes, Mononuclear / Q000378:metabolism; D008099:Liver / Q000378:metabolism*; D008105:Liver Cirrhosis, Biliary / Q000097:blood* / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D014409:Tumor Necrosis Factor-alpha / Q000097:blood / Q000378:metabolism; D055815:Young Adult
chemical_list: C508607:IL9 protein, human; D015850:Interleukin-6; D016906:Interleukin-9; D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10; D007371:Interferon-gamma