title: EFFECT OF NAC ON MOUSE GV OOCYTE SURVIVAL AND SUBSEQUENT EMBRYONIC DEVELOPMENT FOLLOWING VITRFICATION.
abstract: Oocytes that survive cryopreservation may accumulate ROS which are known to bring harmful effects on embryonic development. NAC is an antioxidant which can be a supplement to reduce oxidative stress. However, whether NAC can improve the developmental competence of vitrified GV-oocytes remains unclear.
  
  
  
  The study was to investigate the effect of NAC on subsequent embryonic developmental competence of mice vitrified GV-oocytes.
  
  
  
  This study compared the effects of different concentration of NAC on the cleavage and blastocyst rates of mice vitrified GV-oocytes. Then the effects of NAC on mitochondria distribution, ROS level and embryonic development of vitrified oocytes were tested.
  
  
  
  ROS activity of vitrified oocytes was significantly annihilated and mitochondrial distribution pattern was improved by 1.5 mM NAC (P<0.05). NAC supplementation throughout vitrification/warming and IVM media significantly improved the developmental competence of vitrified oocytes.
  
  
  
  Supplementation of NAC could partially overcome the damages by vitrification and improve the development ability of mice vitrified GV-oocytes.
authors.affiliation: College of Life Science, Northeast Agricultural University, Harbin, Heilongjiang, China.
authors.forename: S L
authors.initials: SL
authors.lastname: Yue
authors.forename: Y T
authors.initials: YT
authors.lastname: Zhang
authors.affiliation: College of Life Science, Northeast Agricultural University, Harbin, Heilongjiang, China.
authors.forename: S W
authors.initials: SW
authors.lastname: Wang
authors.affiliation: College of Life Science, Northeast Agricultural University, Harbin, Heilongjiang, China.
authors.forename: M
authors.initials: M
authors.lastname: Sun
authors.affiliation: College of Life Science, Northeast Agricultural University, Harbin, Heilongjiang, China.
authors.forename: Y C
authors.initials: YC
authors.lastname: Xing
authors.affiliation: College of Life Science, Northeast Agricultural University, Harbin, Heilongjiang, China.
authors.forename: J
authors.initials: J
authors.lastname: Wen
authors.affiliation: College of Life Science, Northeast Agricultural University, Harbin, Heilongjiang, China. jbzhou@neau.edu.cn.
authors.forename: J B
authors.initials: JB
authors.lastname: Zhou
mesh_terms: D000111:Acetylcysteine / Q000494:pharmacology*; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D015925:Cryopreservation / Q000379:methods*; D003451:Cryoprotective Agents / Q000494:pharmacology*; D047108:Embryonic Development / Q000187:drug effects*; D005260:Female; D051379:Mice; D008813:Mice, Inbred ICR; D009865:Oocytes / Q000187:drug effects; D058989:Vitrification / Q000187:drug effects*
chemical_list: D000975:Antioxidants; D003451:Cryoprotective Agents; D000111:Acetylcysteine