title: NMR-Based Metabolomics of Oral Biofluids.
abstract: NMR-based metabolomics is an established technique for characterizing the metabolite profile of biological fluids and investigating how metabolite profiles change in response to biological and/or clinical stimuli. Thus, NMR-based metabolomics has the potential to discover biomarkers for diagnosis, prognosis, and/or therapy of clinical conditions, as well as to unravel the physiology underlying clinical conditions. Here, we describe a detailed protocol for NMR-based metabolomics of oral biofluids, including sample collection, sample handling, NMR data acquisition, and processing. In addition, we give a general overview of the statistical analysis of the resulting metabolomic data.
authors.affiliation: Centre for Advanced Imaging, The University of Queensland, Brisbane, QLD, 4072, Australia. h.schirra@uq.edu.au.
authors.forename: Horst Joachim
authors.initials: HJ
authors.lastname: Schirra
authors.affiliation: School of Dentistry, Oral Health Centre, The University of Queensland, 288 Herston Road, Herston, QLD, 4006, Australia.
authors.forename: Pauline J
authors.initials: PJ
authors.lastname: Ford
mesh_terms: D019295:Computational Biology / Q000379:methods; D003627:Data Interpretation, Statistical; D005883:Gingival Crevicular Fluid / Q000378:metabolism*; D006801:Humans; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D055432:Metabolomics / Q000379:methods*; D020543:Proteome; D012463:Saliva / Q000378:metabolism*
chemical_list: D020543:Proteome
keywords: Gingival crevicular fluid; Metabolomics; NMR spectroscopy; Saliva; Systems biology