title: ROS-INDUCED OXIDATIVE STRESS IN NOBILE-TYPE DENDROBIUM PROTOCORM-LIKE BODIES (PLBS) DURING VITRIFICATION.
abstract: Oxidative stress involved in cryopreservation protocols may be responsible for the poor survival of tissues after cryopreservation.
  
  
  
  In the current study, we aimed to clarify the role of oxidative stress and its relationship with survival rate during cryopreservation of PLBs from nobile-type Dendrobium.
  
  
  
  ROS, antioxidants and oxidative products and the survival rate in PLBs from Dendrobium Hamanal Lake Dream were determined during vitrification.
  
  
  
  Relative survival of PLBs decreased significantly after preculture and rewarming (P<0.01). Generation of ·O2-and protein carbonyl (PCO) increased significantly after preculture. Dramatic increases in ·O2-, H2O2and MDA, and significant decreases in AsA content, activities of SOD and CAT were observed after rewarming.
  
  
  
  ROS-induced oxidative stress was associated with the poor survival of PLBs following vitrification. ·O2-was the predominant ROS resulting in the decreased survival after preculture, while H2O2together with ·O2-appear to be responsible for the survival decrease after rewarming.
authors.affiliation: Beijing Laboratory of Urban and Rural Ecological Environment, Beijing Key Laboratory of Ornamental Plants Germplasm Innovation and Molecular Breeding, National Engineering Research Center for Floriculture, College of Landscape Architecture, Beijing Forestry University, Beijing, China.
authors.forename: M
authors.initials: M
authors.lastname: Jia
authors.affiliation: Beijing Laboratory of Urban and Rural Ecological Environment, Beijing Key Laboratory of Ornamental Plants Germplasm Innovation and Molecular Breeding, National Engineering Research Center for Floriculture, College of Landscape Architecture, Beijing Forestry University, Beijing, China.
authors.forename: W
authors.initials: W
authors.lastname: Di
authors.affiliation: Beijing Laboratory of Urban and Rural Ecological Environment, Beijing Key Laboratory of Ornamental Plants Germplasm Innovation and Molecular Breeding, National Engineering Research Center for Floriculture, College of Landscape Architecture, Beijing Forestry University, Beijing, China. yanwopaper@yahoo.com.
authors.forename: Yan
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Beijing Laboratory of Urban and Rural Ecological Environment, Beijing Key Laboratory of Ornamental Plants Germplasm Innovation and Molecular Breeding, National Engineering Research Center for Floriculture, College of Landscape Architecture, Beijing Forestry University, Beijing, China.
authors.forename: Yin
authors.initials: Y
authors.lastname: Shi
authors.affiliation: Beijing Laboratory of Urban and Rural Ecological Environment, Beijing Key Laboratory of Ornamental Plants Germplasm Innovation and Molecular Breeding, National Engineering Research Center for Floriculture, College of Landscape Architecture, Beijing Forestry University, Beijing, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Xie
mesh_terms: D000975:Antioxidants / Q000378:metabolism; D015925:Cryopreservation; D031667:Dendrobium / Q000502:physiology*; D016877:Oxidants / Q000378:metabolism; D018384:Oxidative Stress; D035281:Plant Tubers / Q000502:physiology; D017382:Reactive Oxygen Species / Q000378:metabolism; D058989:Vitrification
chemical_list: D000975:Antioxidants; D016877:Oxidants; D017382:Reactive Oxygen Species