title: Unc-5 homolog B (UNC5B) is one of the key downstream targets of N-α-Acetyltransferase 10 (Naa10).
abstract: N-α-acetyltransferase 10 (Naa10) displays alpha (N-terminal) acetyltransferase activity. It functions as a major modulator of cell growth and differentiation. Until now, a few downstream targets were found, but no studies have concerned about which gene is the early event of Naa10 downstream target. As we know, the earlier events may play more significant role in Naa10 pathway. Through construction of Naa10 stably knocked down H1299 cell line, we discovered cell morphological changes induced by Naa10. Moreover, potential function of Naa10 in cell morphogenesis was also indicated using cDNA microarray analysis of the Naa10 stably knock-down cell line. We further discovered that netrin-1 (NTN1) and its receptor UNC-5 Homology B (UNC5B) were the early event among the genes involved in Naa10 stably knocked down induced genes expression changes in cell morphogenesis. This was further validated in caudal half region of E10 mouse embryos. Negative regulation of Naa10 towards NTN1 and its receptor UNC5B were also detected upon treatment of all-trans retinoid acid, which was often used to induce morphological differentiation.
authors.affiliation: Department of Obstetrics and Gynecology, Reproductive Medical Center, Peking University Third Hospital, Beijing, China.
authors.forename: Huiyu
authors.initials: H
authors.lastname: Xu
authors.affiliation: Department of pathology, Zhejiang provincial people's hospital, Hangzhou, Zhejiang Province, P.R. China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Han
authors.affiliation: 307-Ivy Translational Medicine Center, Laboratory of Oncology, Affiliated Hospital of Academy of Military Medical Sciences, Beijing, China.
authors.forename: Bing
authors.initials: B
authors.lastname: Liu
authors.affiliation: Department of Obstetrics and Gynecology, Reproductive Medical Center, Peking University Third Hospital, Beijing, China.
authors.forename: Rong
authors.initials: R
authors.lastname: Li
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D004622:Embryo, Mammalian / Q000166:cytology; D042783:Endothelial Cells / Q000378:metabolism; D005260:Female; D020869:Gene Expression Profiling; D055785:Gene Knockdown Techniques; D008810:Mice, Inbred C57BL; D063209:N-Terminal Acetyltransferase A / Q000378:metabolism*; D063213:N-Terminal Acetyltransferase E / Q000378:metabolism*; D000075386:Netrin Receptors / Q000378:metabolism*; D000075388:Netrin-1 / Q000378:metabolism; D034741:RNA, Small Interfering / Q000378:metabolism; D011956:Receptors, Cell Surface / Q000378:metabolism*; D015203:Reproducibility of Results; D014162:Transfection
chemical_list: D000075386:Netrin Receptors; D034741:RNA, Small Interfering; D011956:Receptors, Cell Surface; C532445:UNC5B protein, human; C000617307:Unc5b protein, mouse; D000075388:Netrin-1; D063209:N-Terminal Acetyltransferase A; C504824:NAA10 protein, human; C508558:Naa10 protein, mouse; D063213:N-Terminal Acetyltransferase E