title: Quantitative impact of influent characteristics on nitrogen removal via anammox and denitrification in a landfill bioreactor case.
abstract: In this paper, Quantitative Polymerase Chain Reaction (qPCR), Illumina MiSeq sequencing and15N stable isotopic tracing methods were applied to study the effects of influent organic loading, BOD5/TN and NO2-N/NH4-N ratios on the combined nitrogen removal through denitrification and anammox. The results show that, the total nitrogen removal was above 90% at the optimum reaction condition: organic loading 0.04kg/m3·day, BOD5/TN 0.2, and NO2-N/NH4-N 1.0. Approximately 9.43% of the nitrogen removal occurred through anammox process when BOD5/TN was 0.1, and increased to 21.46% when BOD5/TN was 0.2. The anammox accounted for 10% when NO2-N/NH4-N was 0.5, and that increased to 20.72% when NO2-N/NH4-N was 1.0-1.5. hzsA was directly proportional to the influent BOD5/TN. A positive correlation between BOD5/TN and anammox microorganisms existed. Similarly, there was a positive correlation between NO2-N/NH4-N and denitrification microorganisms. Therefore, it is feasible to regulate the influent to improve nitrogen removal efficiency.
authors.affiliation: Key Laboratory of Urbanization and Ecological Restoration of Shanghai, School of Ecology & Environmental Science, East China Normal University, Shanghai 200241, China.
authors.forename: Xiaoyuan
authors.initials: X
authors.lastname: Wang
authors.affiliation: Key Laboratory of Urbanization and Ecological Restoration of Shanghai, School of Ecology & Environmental Science, East China Normal University, Shanghai 200241, China. Electronic address: bxie@des.ecnu.edu.cn.
authors.forename: Bing
authors.initials: B
authors.lastname: Xie
authors.affiliation: Key Laboratory of Urbanization and Ecological Restoration of Shanghai, School of Ecology & Environmental Science, East China Normal University, Shanghai 200241, China.
authors.forename: Chaoqun
authors.initials: C
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Urbanization and Ecological Restoration of Shanghai, School of Ecology & Environmental Science, East China Normal University, Shanghai 200241, China.
authors.forename: Yiwen
authors.initials: Y
authors.lastname: Shen
authors.affiliation: School of Science and School of Interprofessional Health Studies, Faculty of Health and Environmental Sciences, Auckland University of Technology, Auckland 1142, New Zealand; Institute of Biomedical Technology, Auckland University of Technology, Auckland 1142, New Zealand.
authors.forename: Jun
authors.initials: J
authors.lastname: Lu
mesh_terms: D000641:Ammonia / Q000032:analysis / Q000378:metabolism; D019149:Bioreactors; D058440:Denitrification; D009584:Nitrogen / Q000032:analysis / Q000378:metabolism; D064091:Waste Disposal Facilities
chemical_list: D000641:Ammonia; D009584:Nitrogen
keywords: Anammox; Denitrification; Functional microbial; Influent characteristics; Organic loading