title: Cell death affected by dental alloys: Modes and mechanisms.
abstract: Previous reports have demonstrated that ions released from dental alloys might cause cytotoxicity. However, how dental alloys influence the organism has not been extensively studied. In order to make it clear, the cytotoxic effect of four dental alloys on L929 cells was evaluated by flow cytometry (FCM) and Real-time quantitative PCR assay (Real-time qPCR) to identify the cell death mode and its biological mechanism. The cells were treated with the leach liquors of cobalt-chromium (Co-Cr), commercially pure titanium (CP-Ti), palladium-based (Pd-based) and gold-platinum (Au-Pt) alloys for 48 and 72 h. FCM results indicated, apart from Au-Pt alloy, the major cell death of dental alloys was time-dependent early apoptosis rather than necrosis/late apoptosis. Caspase 3 and Caspase 9 mRNA expression were determined by Real-time qPCR, and shared the same trend in each group over time. Hence, except for Au-Pt alloy, dental alloys might cause time-dependent early apoptosis via the intrinsic pathway.
authors.affiliation: School of Stomatology, Fujian Medical University.
authors.forename: Yu
authors.initials: Y
authors.lastname: Pan
authors.forename: Lei
authors.initials: L
authors.lastname: Jiang
authors.forename: Honglei
authors.initials: H
authors.lastname: Lin
authors.forename: Hui
authors.initials: H
authors.lastname: Cheng
mesh_terms: D016923:Cell Death; D002858:Chromium Alloys; D003722:Dental Alloys; D006047:Gold Alloys; D006801:Humans; D008422:Materials Testing; D010984:Platinum; D014025:Titanium
chemical_list: D002858:Chromium Alloys; D003722:Dental Alloys; D006047:Gold Alloys; D010984:Platinum; D014025:Titanium