title: Fate of tetracycline resistance in synthetic livestock carcass leachate for two years.
abstract: To simulate the fate of antibiotic resistance in leachate from anaerobic carcass landfill site, anaerobic reactors were set-up and their antibiotic resistance activities were monitored for 2 years. Initially, Escherichia coli DH5α with tetracycline resistance pB10 plasmid was inoculated in nutrient rich anaerobic reactors. The fate of tetracycline resistant bacteria (TRB) was tracked by analysis using culture-based method, EC50(half maximal effective concentration), and quantitative polymerase chain reaction (qPCR). Based on the temporal pattern of EC50during the study period, TRB continuously increased during Phase I (0-250th day), went down in Phase II (after 250th day to 500th day), and then increased again by the end of Phase III (after 500th day to the 774th day). Interestingly, pB10 plasmid accumulated in the system as the community diversities increased over time. At the end of experiment, the tetracycline resistance microbial communities were investigated by 16s RNA gene-based pyro sequencing. The results of this study indicated that leachate with high organic strength in anaerobic conditions could be an antibiotic resistant point source in several year periods.
authors.affiliation: Program in Environmental Technology and Policy, Korea University, Sejong, 30019, Republic of Korea.
authors.forename: Dennis Espineli
authors.initials: DE
authors.lastname: Salcedo
authors.affiliation: Program in Environmental Technology and Policy, Korea University, Sejong, 30019, Republic of Korea; Department of Environmental Engineering, College of Science and Technology, Korea University, Sejong, 30019, Republic of Korea. Electronic address: ub1905ub@korea.ac.kr.
authors.forename: Sungpyo
authors.initials: S
authors.lastname: Kim
mesh_terms: D000693:Anaerobiosis; D000818:Animals; D000900:Anti-Bacterial Agents / Q000032:analysis / Q000737:chemistry; D057919:Biological Oxygen Demand Analysis; D004926:Escherichia coli / Q000235:genetics*; D058751:Livestock; D010957:Plasmids / Q000235:genetics; D016133:Polymerase Chain Reaction; D012336:RNA, Ribosomal, 16S / Q000032:analysis; D013752:Tetracycline / Q000032:analysis / Q000737:chemistry*; D013753:Tetracycline Resistance; D013997:Time Factors
chemical_list: D000900:Anti-Bacterial Agents; D012336:RNA, Ribosomal, 16S; D013752:Tetracycline
keywords: Escherichia coli; FMD; Leachate; Plasmid; TRB; Tetracycline