title: Targeted disruption of the orphan receptor Gpr151 does not alter pain-related behaviour despite a strong induction in dorsal root ganglion expression in a model of neuropathic pain.
abstract: Gpr151 is an orphan GPCR whose function is unknown. The restricted pattern of neuronal expression in the habenula, dorsal horn of the spinal cord and dorsal root ganglion plus homology with the galanin family of receptors imply a role in nociception.
  
  
  
  Real-time quantitative RT-PCR demonstrated a 49.9±2.9 fold highly significant (P<0.001) increase in Gpr151 mRNA expression in the dorsal root ganglion 7days after the spared nerve injury model of neuropathic pain. Measures of acute, inflammatory and neuropathic pain behaviours were not significantly different using separate groups of Gpr151 loss-of-function mutant mice and wild-type controls. Galanin at concentrations between 100nM and 10μM did not induce calcium signalling responses in ND7/23 cells transfected with Gpr151.
  
  
  
  Our results indicate that despite the very large upregulation in the DRG after a nerve injury model of neuropathic pain, the Gpr151 orphan receptor does not appear to be involved in the modulation of pain-related behaviours. Further, galanin is unlikely to be an endogenous ligand for Gpr151.
authors.affiliation: School of Physiology, Pharmacology & Neuroscience, Medical Sciences Building, University Walk, Bristol BS8 1TD, UK; School of Clinical Sciences, Medical Sciences Building, University Walk, Bristol BS8 1TD, UK.
authors.forename: Fiona E
authors.initials: FE
authors.lastname: Holmes
authors.affiliation: School of Physiology, Pharmacology & Neuroscience, Medical Sciences Building, University Walk, Bristol BS8 1TD, UK; School of Clinical Sciences, Medical Sciences Building, University Walk, Bristol BS8 1TD, UK.
authors.forename: Niall
authors.initials: N
authors.lastname: Kerr
authors.affiliation: School of Physiology, Pharmacology & Neuroscience, Medical Sciences Building, University Walk, Bristol BS8 1TD, UK; School of Clinical Sciences, Medical Sciences Building, University Walk, Bristol BS8 1TD, UK.
authors.forename: Ying-Ju
authors.initials: YJ
authors.lastname: Chen
authors.affiliation: School of Physiology, Pharmacology & Neuroscience, Medical Sciences Building, University Walk, Bristol BS8 1TD, UK; School of Clinical Sciences, Medical Sciences Building, University Walk, Bristol BS8 1TD, UK.
authors.forename: Penny
authors.initials: P
authors.lastname: Vanderplank
authors.affiliation: School of Clinical Sciences, Dorothy Hodgkin Building, Whitson Street, Bristol BS1 3NY, UK.
authors.forename: Craig A
authors.initials: CA
authors.lastname: McArdle
authors.affiliation: School of Physiology, Pharmacology & Neuroscience, Medical Sciences Building, University Walk, Bristol BS8 1TD, UK; School of Clinical Sciences, Medical Sciences Building, University Walk, Bristol BS8 1TD, UK. Electronic address: d.wynick@bristol.ac.uk.
authors.forename: David
authors.initials: D
authors.lastname: Wynick
mesh_terms: D000818:Animals; D020013:Calcium Signaling; D045744:Cell Line, Tumor; D019004:Galanin / Q000378:metabolism; D005727:Ganglia, Spinal / Q000378:metabolism* / Q000502:physiology; D051379:Mice; D009154:Mutation; D009437:Neuralgia / Q000378:metabolism*; D011485:Protein Binding; D043562:Receptors, G-Protein-Coupled / Q000235:genetics / Q000378:metabolism*
chemical_list: C000625968:Gpr151 protein, mouse; D043562:Receptors, G-Protein-Coupled; D019004:Galanin
keywords: Dorsal root ganglion; GalR1; GalR2; Galanin; Gpr151; Pain