title: Polysaccharides from Arnebia euchroma Ameliorated Endotoxic Fever and Acute Lung Injury in Rats Through Inhibiting Complement System.
abstract: Arnebiaeuchroma (Royle) Johnst (Ruanzicao) is a traditional Chinese herbal medicine (TCM). It is extensively used in China and other countries for treatment of inflammatory diseases. It is known that hyper-activated complement system involves in the fever and acute lung injury (ALI) in rats. In our preliminary studies, anti-complementary activity of crude Arnebiaeuchroma polysaccharides (CAEP) had been demonstrated in vitro. This study aimed to investigate the role and mechanism of crude Arnebiaeuchroma polysaccharides (CAEP) using two animal models, which relate with inappropriate activation of complement system. In lipopolysaccharide (LPS)-induced fever model, the body temperature and leukocytes of peripheral blood in rats were significantly increased, while the complement levels of serum were remarkably decreased. CAEP administration alleviated the LPS-induced fever, reduced the number of leukocytes, and improved the levels of complement. Histological assay showed that there were severe damages and complement depositions in lung of the ALI rats. Further detection displayed that the oxidant stress was enhanced, and total hemolytic activity and C3/C4 levels in serum were decreased significantly in the ALI model group. Remarkably, CAEP not only attenuated the morphological injury, edema, and permeability in the lung but also significantly weakened the oxidant stress in bronchoalveolar lavage fluid (BALF) in the ALI rats. The levels of complement and complement depositions were improved by the CAEP treatment. In conclusion, the CAEP treatment ameliorated febrile response induced by LPS and acute lung injury induced by LPS plus ischemia-reperfusion. CAEP exerted beneficial effects on inflammatory disease potentially via inhibiting the inappropriate activation of complement system.
authors.affiliation: Department of Pharmacology, School of Pharmacy, Fudan University, Shanghai, 201203, China.
authors.forename: Ying-Ye
authors.initials: YY
authors.lastname: Ou
authors.affiliation: Department of Pharmacognosy, School of Pharmacy, Fudan University, Shanghai, 201203, China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Jiang
authors.affiliation: Department of Pharmacology, School of Pharmacy, Fudan University, Shanghai, 201203, China. lxzhang@shmu.edu.cn.
authors.forename: Hong
authors.initials: H
authors.lastname: Li
authors.affiliation: Department of Pharmacology, School of Pharmacy, Fudan University, Shanghai, 201203, China.
authors.forename: Yun-Yi
authors.initials: YY
authors.lastname: Zhang
authors.affiliation: Department of Pharmacognosy, School of Pharmacy, Fudan University, Shanghai, 201203, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Lu
authors.affiliation: Department of Pharmacognosy, School of Pharmacy, Fudan University, Shanghai, 201203, China. dfchen@shmu.edu.cn.
authors.forename: Dao-Feng
authors.initials: DF
authors.lastname: Chen
mesh_terms: D055371:Acute Lung Injury / Q000139:chemically induced / Q000188:drug therapy*; D000818:Animals; D028082:Boraginaceae / Q000737:chemistry*; D003167:Complement Activation / Q000187:drug effects*; D051056:Complement Inactivating Agents / Q000494:pharmacology; D003165:Complement System Proteins / Q000187:drug effects; D005334:Fever / Q000139:chemically induced / Q000188:drug therapy*; D008070:Lipopolysaccharides; D008516:Medicine, Chinese Traditional / Q000379:methods; D008517:Phytotherapy / Q000379:methods; D011134:Polysaccharides / Q000008:administration & dosage / Q000627:therapeutic use*; D051381:Rats
chemical_list: D051056:Complement Inactivating Agents; D008070:Lipopolysaccharides; D011134:Polysaccharides; D003165:Complement System Proteins
keywords: Arnebia euchroma; acute lung injury; complement; fever; polysaccharides