title: Transcriptome differences between fiber-type and seed-typeCannabis sativavariety exposed to salinity.
abstract: The industrial hemp varieties 'Yunma 5' and 'Bamahuoma,' which demonstrate growth vigor and environmental adaptability, have been primarily cultivated in Yunnan and Guangxi, China, respectively, for fiber and seeds. The results of physiological measurements showed the phenotypic differences between the two varieties in response to salt stress. RNA-Seq analysis was first performed on leaves of both varieties sampled at four time intervals (0, 2, 4, 6 days) after treatment with salt (500 mM NaCl) We identified 220 co-up-regulated differentially expressed genes (DEGs) in the two varieties, while 26 up-regulated DEGs and 24 down-regulated DEGs were identified exclusively in the single varieties after 2 days of salt stress. Among the 220 DEGs, we identified 22 transcription factors, including key transcription factors involved in salt stress, such as MYB, NAC, GATA, and HSF. We applied gene expression profile analysis and found that 'Yunma 5' and 'Bamahuoma' have variety-specific pathways for resisting salt stress. The DEGs of 'Yunma 5' were enriched in spliceosome and amino acid metabolism genes, while the DEGs of 'Bamahuoma' were enriched in fatty acid metabolism, amino acid metabolism, and endoplasmic reticulum protein processing pathway. Although there were common DEGs, such as genes encoding cysteine protease and alpha/beta-hydrolase superfamily, the two varieties' responses to salt stress impacted different metabolic pathways. The DEGs that were co-expressed in both varieties under stress may provide useful insights into the tolerance of cultivated hemp and other bast fiber crops to saline soil conditions. These transcriptomes also represent reference sequences for industrial hemp.
authors.affiliation: Plant Improvement and Utilization Lab, Yunnan University, Kunming, 650091 Yunnan China.
authors.forename: Jiajia
authors.initials: J
authors.lastname: Liu
authors.affiliation: Plant Improvement and Utilization Lab, Yunnan University, Kunming, 650091 Yunnan China.
authors.forename: Qin
authors.initials: Q
authors.lastname: Qiao
authors.affiliation: Plant Improvement and Utilization Lab, Yunnan University, Kunming, 650091 Yunnan China.
authors.forename: Xia
authors.initials: X
authors.lastname: Cheng
authors.affiliation: Plant Improvement and Utilization Lab, Yunnan University, Kunming, 650091 Yunnan China.
authors.forename: Guanghui
authors.initials: G
authors.lastname: Du
authors.affiliation: Plant Improvement and Utilization Lab, Yunnan University, Kunming, 650091 Yunnan China.
authors.forename: Gang
authors.initials: G
authors.lastname: Deng
authors.affiliation: Kunming Medical University Haiyuan College, Kunming, 650106 Yunnan China.
authors.forename: Mingzhi
authors.initials: M
authors.lastname: Zhao
authors.affiliation: Plant Improvement and Utilization Lab, Yunnan University, Kunming, 650091 Yunnan China.
authors.forename: Feihu
authors.initials: F
authors.lastname: Liu
keywords: Differential expression gene; Industrial hemp; RNA-Seq; Salt stress