title: Maternal High Fat Diet Alters Skeletal Muscle Mitochondrial Catalytic Activity in Adult Male Rat Offspring.
abstract: A maternal high-fat (HF) diet during pregnancy can lead to metabolic compromise, such as insulin resistance in adult offspring. Skeletal muscle mitochondrial dysfunction is one mechanism contributing to metabolic impairments in insulin resistant states. Therefore, the present study aimed to investigate whether mitochondrial dysfunction is evident in metabolically compromised offspring born to HF-fed dams. Sprague-Dawley dams were randomly assigned to receive a purified control diet (CD; 10% kcal from fat) or a high fat diet (HFD; 45% kcal from fat) for 10 days prior to mating, throughout pregnancy and during lactation. From weaning, all male offspring received a standard chow diet and soleus muscle was collected at day 150. Expression of the mitochondrial transcription factors nuclear respiratory factor-1 (NRF1) and mitochondrial transcription factor A (mtTFA) were downregulated in HF offspring. Furthermore, genes encoding the mitochondrial electron transport system (ETS) respiratory complex subunits were suppressed in HF offspring. Moreover, protein expression of the complex I subunit, NDUFB8, was downregulated in HF offspring (36%), which was paralleled by decreased maximal catalytic linked activity of complex I and III (40%). Together, these results indicate that exposure to a maternal HF diet during development may elicit lifelong mitochondrial alterations in offspring skeletal muscle.
authors.affiliation: Liggins Institute, University of Auckland Auckland, New Zealand.
authors.forename: Chantal A
authors.initials: CA
authors.lastname: Pileggi
authors.affiliation: College of Sport and Exercise Science, Institute of Sport, Exercise and Active Living, Victoria UniversityMelbourne, VIC, Australia; Applied Surgery and Metabolism Laboratory, School of Biological Sciences, University of AucklandAuckland, New Zealand.
authors.forename: Christopher P
authors.initials: CP
authors.lastname: Hedges
authors.affiliation: Liggins Institute, University of AucklandAuckland, New Zealand; Gravida: National Centre for Growth and Development, University of AucklandAuckland, New Zealand.
authors.forename: Stephanie A
authors.initials: SA
authors.lastname: Segovia
authors.affiliation: Liggins Institute, University of Auckland Auckland, New Zealand.
authors.forename: James F
authors.initials: JF
authors.lastname: Markworth
authors.affiliation: Liggins Institute, University of Auckland Auckland, New Zealand.
authors.forename: Brenan R
authors.initials: BR
authors.lastname: Durainayagam
authors.affiliation: Liggins Institute, University of AucklandAuckland, New Zealand; Gravida: National Centre for Growth and Development, University of AucklandAuckland, New Zealand.
authors.forename: Clint
authors.initials: C
authors.lastname: Gray
authors.affiliation: Liggins Institute, University of AucklandAuckland, New Zealand; Gravida: National Centre for Growth and Development, University of AucklandAuckland, New Zealand.
authors.forename: Xiaoyuan D
authors.initials: XD
authors.lastname: Zhang
authors.affiliation: Food Nutrition and Health Team, Food and Bio-based Products Group, AgResearch Grasslands Palmerston North, New Zealand.
authors.forename: Matthew P G
authors.initials: MP
authors.lastname: Barnett
authors.affiliation: Liggins Institute, University of AucklandAuckland, New Zealand; Gravida: National Centre for Growth and Development, University of AucklandAuckland, New Zealand.
authors.forename: Mark H
authors.initials: MH
authors.lastname: Vickers
authors.affiliation: Applied Surgery and Metabolism Laboratory, School of Biological Sciences, University of Auckland Auckland, New Zealand.
authors.forename: Anthony J R
authors.initials: AJ
authors.lastname: Hickey
authors.affiliation: Liggins Institute, University of AucklandAuckland, New Zealand; Gravida: National Centre for Growth and Development, University of AucklandAuckland, New Zealand.
authors.forename: Clare M
authors.initials: CM
authors.lastname: Reynolds
authors.affiliation: Liggins Institute, University of Auckland Auckland, New Zealand.
authors.forename: David
authors.initials: D
authors.lastname: Cameron-Smith
keywords: developmental programming; maternal high fat; mitochondria; skeletal muscle