title: Cross-protection against Vibrio cholerae infection by monoclonal antibodies against Vibrio vulnificus RtxA1/MARTXVv.
abstract: Gram-negative Vibrio species secrete multifunctional autoprocessing repeats-in-toxin (MARTX) toxins associated with bacterial pathogenesis. Here, the cross-reactivity and cross-protectivity of mAbs against V. vulnificus RtxA1/MARTXVvwas evaluated. Passive administration of any of these mAbs (21RA, 24RA, 46RA, 47RA and 50RA) provided strong protection against lethal V. cholerae infection. Interestingly, 24RA and 46RA, which map to the cysteine protease domain of V. cholerae MARTXVc, inhibited CPD autocleavage in vitro; this process is involved in V. cholerae pathogenesis. These results generate new insight into the development of broadly protective mAbs and/or vaccines against Vibrio species with MARTX toxins.
authors.affiliation: Department of Microbiology and Immunology, Chonbuk National University Medical School, Jeonju, Jeonbuk 54896, Republic of Korea.|Institute for Medical Science, Chonbuk National University Medical School, Jeonju, Jeonbuk 54896, Republic of Korea.
authors.forename: Tae Hee
authors.initials: TH
authors.lastname: Lee
authors.affiliation: Department of Chemistry and Nano Science, Ewha Womans University, Seoul 03760, Republic of Korea.
authors.forename: Sun-Shin
authors.initials: SS
authors.lastname: Cha
authors.affiliation: Department of Internal Medicine, Chonbuk National University Medical School, Jeonju, Jeonbuk 54896, Republic of Korea.
authors.forename: Chang-Seop
authors.initials: CS
authors.lastname: Lee
authors.affiliation: Department of Microbiology and Clinical Vaccine R&D Center, Chonnam National University Medical School, Gwangju 61469, Republic of Korea.
authors.forename: Joon Haeng
authors.initials: JH
authors.lastname: Rhee
authors.affiliation: Department of New Biology, DGIST, Daegu 42988, Republic of Korea.
authors.forename: Hye Ryun
authors.initials: HR
authors.lastname: Woo
authors.affiliation: Department of Microbiology and Immunology, Chonbuk National University Medical School, Jeonju, Jeonbuk 54896, Republic of Korea.|Institute for Medical Science, Chonbuk National University Medical School, Jeonju, Jeonbuk 54896, Republic of Korea.
authors.forename: Kyung Min
authors.initials: KM
authors.lastname: Chung
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000008:administration & dosage / Q000276:immunology; D000911:Antibodies, Monoclonal / Q000008:administration & dosage / Q000276:immunology*; D001427:Bacterial Toxins / Q000235:genetics / Q000276:immunology; D002771:Cholera / Q000276:immunology* / Q000401:mortality / Q000517:prevention & control*; D056738:Cross Protection; D004195:Disease Models, Animal; D051379:Mice; D009154:Mutation; D014734:Vibrio cholerae / Q000235:genetics / Q000276:immunology*; D041261:Vibrio vulnificus / Q000235:genetics / Q000276:immunology*
chemical_list: D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D001427:Bacterial Toxins
keywords: MARTX toxin; Vibrio species; cross-protection; monoclonal antibody