title: Effects of dietary heme iron and exercise training on abdominal fat accumulation and lipid metabolism in high-fat diet-fed mice.
abstract: Animal by-products can be recycled and used as sources of essential nutrients. Water-soluble heme iron (WSHI), a functional food additive for supplementing iron, is produced by processing animal blood. In this study, we investigated the effects of dietary supplementation of 3% WSHI and exercise training for 4 weeks on the accumulation of abdominal fat and lipid metabolism in mice fed high-fat diet. Exercise-trained mice had significantly less perirenal adipose tissue, whereas WSHI-fed mice tended to have less epididymal adipose tissue. In addition, total weight of abdominal adipose tissues was significantly decreased in the Exercise + WSHI group. Dietary WSHI significantly increased the messenger RNA (mRNA) levels of lipoprotein lipase and hormone-sensitive lipase. WSHI-fed mice also tended to show increased mRNA levels of adipose triglyceride lipase in their epididymal adipose tissue. Dietary WSHI also significantly decreased the mRNA levels of fatty acid oxidation-related enzymes in the liver, but did not influence levels in the Gastrocnemius muscle. Exercise training did not influence the mRNA levels of lipid metabolism-related enzymes in the epididymal adipose tissue, liver or the Gastrocnemius muscle. These findings suggest that the accumulation of abdominal fat can be efficiently decreased by the combination of dietary WSHI and exercise training in mice fed high-fat diet.
authors.affiliation: Graduate School of Agricultural Science, Kobe University, Kobe, Japan.
authors.forename: Masanori
authors.initials: M
authors.lastname: Katsumura
authors.affiliation: Graduate School of Agricultural Science, Kobe University, Kobe, Japan.
authors.forename: Shoko
authors.initials: S
authors.lastname: Takagi
authors.affiliation: Graduate School of Agricultural Science, Kobe University, Kobe, Japan.
authors.forename: Hana
authors.initials: H
authors.lastname: Oya
authors.affiliation: Graduate School of Agricultural Science, Kobe University, Kobe, Japan.
authors.forename: Shohei
authors.initials: S
authors.lastname: Tamura
authors.affiliation: Graduate School of Agricultural Science, Kobe University, Kobe, Japan.
authors.forename: Takaoki
authors.initials: T
authors.lastname: Saneyasu
authors.affiliation: Graduate School of Agricultural Science, Kobe University, Kobe, Japan.
authors.forename: Kazuhisa
authors.initials: K
authors.lastname: Honda
authors.affiliation: Graduate School of Agricultural Science, Kobe University, Kobe, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Kamisoyama
mesh_terms: D050153:Abdominal Fat / Q000378:metabolism*; D000273:Adipose Tissue / Q000201:enzymology; D000818:Animals; D059305:Diet, High-Fat; D019587:Dietary Supplements; D006418:Heme / Q000008:administration & dosage*; D006801:Humans; D007501:Iron / Q000008:administration & dosage*; D008049:Lipase / Q000235:genetics / Q000378:metabolism; D050356:Lipid Metabolism / Q000502:physiology*; D008071:Lipoprotein Lipase / Q000235:genetics / Q000378:metabolism; D008297:Male; D008813:Mice, Inbred ICR; D010805:Physical Conditioning, Animal / Q000502:physiology*; D012995:Solubility; D044767:Ubiquitin-Protein Ligases; D014867:Water; D000217:Acyltransferases
chemical_list: D006418:Heme; D007501:Iron; D008049:Lipase; D008071:Lipoprotein Lipase; D044767:Ubiquitin-Protein Ligases; D014867:Water; C000603471:RNF125 protein, mouse; C494445:PNPLA2 protein, mouse; D000217:Acyltransferases
keywords: exercise; fat; heme iron; mice