title: Detection of Prostate Specific Membrane Antigen at Picomolar Levels Using Biocatalysis Coupled to Assisted Ion Transfer Voltammetry at a Liquid-Organogel Microinterface Array.
abstract: A label-free electrochemical strategy for the detection of a cancer biomarker, prostate specific membrane antigen (PSMA), at picomolar concentrations without the use of antibodies, was investigated. The approach is based on the assisted ion transfer of protons, generated by a series of enzymatic reactions, at an array of microinterfaces between two immiscible electrolyte solutions (μ-ITIES). This nonredox electrochemical approach based on biocatalysis-coupled proton transfer at the μ-ITIES array opens a new way to detect the prostate cancer biomarker, with detection capability achieved at concentrations below those indicative of disease presence. The strategy is expected to contribute to cancer diagnostics, recurrence monitoring, and therapeutic treatment efficacy.
authors.affiliation: Nanochemistry Research Institute & Department of Chemistry, Curtin University , G.P.O Box U1987, Perth, Western Australia 6845, Australia.
authors.forename: Rashida
authors.initials: R
authors.lastname: Akter
authors.affiliation: Nanochemistry Research Institute & Department of Chemistry, Curtin University , G.P.O Box U1987, Perth, Western Australia 6845, Australia.
authors.forename: Damien W M
authors.initials: DW
authors.lastname: Arrigan
mesh_terms: D000954:Antigens, Surface / Q000032:analysis*; D055162:Biocatalysis; D014408:Biomarkers, Tumor / Q000032:analysis*; D055664:Electrochemical Techniques; D005782:Gels / Q000737:chemistry; D043425:Glutamate Carboxypeptidase II / Q000032:analysis*; D006801:Humans; D008297:Male; D011471:Prostatic Neoplasms / Q000000981:diagnostic imaging / Q000201:enzymology*
chemical_list: D000954:Antigens, Surface; D014408:Biomarkers, Tumor; D005782:Gels; C473848:FOLH1 protein, human; D043425:Glutamate Carboxypeptidase II