title: Efflux mediated colistin resistance in diverse clones of Klebsiella pneumoniae from aquatic environment.
abstract: The peptide drug colistin is commonly used to treat carbapenem resistant gram negative bacterial infections. In the present study, we report efflux mediated colistin resistance in multidrug resistant Klebsiella pneumoniae isolates belonging to ST200 and ST1296, isolated from a fresh water environment. The isolates exhibited intermediate resistance to human serum, possessed Type 1 fimbriae and harbored blaSHV-34and blaTEM-1genes. Our results highlight the evolving nature of these clones in the country. These observations emphasize the need for judicious usage of antibiotics to prevent the imminent danger of losing out on currently available therapeutic options.
authors.affiliation: School of Biological Sciences, National Institute of Science Education and Research Bhubaneswar, HBNI, Room No. 407, 3rd Floor, Khurda, Jatni, 752050, India.
authors.forename: Santosh Kumar
authors.initials: SK
authors.lastname: Singh
authors.affiliation: School of Biological Sciences, National Institute of Science Education and Research Bhubaneswar, HBNI, Room No. 407, 3rd Floor, Khurda, Jatni, 752050, India.
authors.forename: Mitali
authors.initials: M
authors.lastname: Mishra
authors.affiliation: School of Biological Sciences, National Institute of Science Education and Research Bhubaneswar, HBNI, Room No. 407, 3rd Floor, Khurda, Jatni, 752050, India.
authors.forename: Minu
authors.initials: M
authors.lastname: Sahoo
authors.affiliation: School of Biological Sciences, National Institute of Science Education and Research Bhubaneswar, HBNI, Room No. 407, 3rd Floor, Khurda, Jatni, 752050, India.
authors.forename: Shashank
authors.initials: S
authors.lastname: Patole
authors.affiliation: School of Biological Sciences, National Institute of Science Education and Research Bhubaneswar, HBNI, Room No. 407, 3rd Floor, Khurda, Jatni, 752050, India. Electronic address: hm@niser.ac.in.
authors.forename: Harapriya
authors.initials: H
authors.lastname: Mohapatra
mesh_terms: D000595:Amino Acid Sequence; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D003091:Colistin / Q000494:pharmacology*; D024881:Drug Resistance, Bacterial; D007711:Klebsiella pneumoniae / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism*; D008826:Microbial Sensitivity Tests; D010641:Phenotype; D037521:Virulence Factors / Q000235:genetics; D014871:Water Microbiology
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D037521:Virulence Factors; D003091:Colistin
keywords: Colistin resistance; Efflux pump; Klebsiella pneumoniae; Sequence type