title: Chemoprevention of spontaneous ovarian cancer in the domestic hen.
abstract: The hen is an attractive animal model for in vivo testing of agents that thwart ovarian carcinogenesis because ovarian cancer in the domestic hen features clinical and molecular alterations that are similar to ovarian cancer in humans, including a high incidence of p53 mutations. The objective of the study was to test the potential ovarian cancer chemopreventive effect of the p53 stabilizing compound CP-31398 on hens that spontaneously present the ovarian cancer phenotype. Beginning at 79 wk of age, 576 egg-laying hens (Gallus domesticus) were randomized to diets containing different amounts of CP-31398 for 94 wk, 5 d, comprising a control group (C) (n = 144), which was fed a diet containing 0 ppm (mg/kg) of CP-31398; a low-dose treatment (LDT) group (n = 144), which was fed a diet containing 100 ppm of CP-31398; a moderate-dose treatment (MDT) group (n = 144) which was fed a diet containing 200 ppm of CP-31398; and a high-dose treatment (HDT) group (n = 144), which was fed a diet containing 300 ppm of CP-31398. Hens were killed at 174 wk of age to determine the incidence of ovarian and oviductal adenocarcinomas. Whereas the incidence of localized and metastatic ovarian cancers in the MDT and HDT groups was significantly lower (up to 77%) compared to levels in the C and LDT groups (P < 0.05), the incidence of oviductal cancer was unaffected by CP-31398. CP-31398 appears to be an effective tool for chemoprevention against ovarian malignancies, but does not appear to affect oviductal malignancies.
authors.affiliation: Prestage Department of Poultry Science, North Carolina State University, Raleigh, 27695.|Graduate Physiology Program, North Carolina State University, Raleigh, 27695.
authors.forename: E H
authors.initials: EH
authors.lastname: Mocka
authors.affiliation: Prestage Department of Poultry Science, North Carolina State University, Raleigh, 27695.|Graduate Physiology Program, North Carolina State University, Raleigh, 27695.
authors.forename: R A
authors.initials: RA
authors.lastname: Stern
authors.affiliation: Department of Population Health and Pathobiology, College of Veterinary Medicine, North Carolina State University, Raleigh, 27695.
authors.forename: O J
authors.initials: OJ
authors.lastname: Fletcher
authors.affiliation: Prestage Department of Poultry Science, North Carolina State University, Raleigh, 27695.|Graduate Physiology Program, North Carolina State University, Raleigh, 27695.
authors.forename: K E
authors.initials: KE
authors.lastname: Anderson
authors.affiliation: Prestage Department of Poultry Science, North Carolina State University, Raleigh, 27695.|Graduate Physiology Program, North Carolina State University, Raleigh, 27695.
authors.forename: J N
authors.initials: JN
authors.lastname: Petitte
authors.affiliation: Prestage Department of Poultry Science, North Carolina State University, Raleigh, 27695.|Graduate Physiology Program, North Carolina State University, Raleigh, 27695.
authors.forename: P E
authors.initials: PE
authors.lastname: Mozdziak
mesh_terms: D000230:Adenocarcinoma / Q000517:prevention & control* / Q000662:veterinary*; D000821:Animal Feed / Q000032:analysis; D000818:Animals; D018890:Chemoprevention / Q000662:veterinary*; D002645:Chickens; D004032:Diet / Q000662:veterinary; D005260:Female; D016158:Genes, p53 / Q000187:drug effects; D005833:Genital Neoplasms, Female / Q000517:prevention & control / Q000662:veterinary; D010051:Ovarian Neoplasms / Q000517:prevention & control* / Q000662:veterinary*; D010057:Oviducts / Q000473:pathology; D010058:Oviposition; D011743:Pyrimidines / Q000008:administration & dosage / Q000494:pharmacology*
chemical_list: D011743:Pyrimidines; C402665:CP 31398
keywords: CP-31398; avian; cancer; ovarian; oviduct