title: Preclinical Study of Anticoagulation Regimens in Sheep After Implantation of CH-VAD Blood Pump.
abstract: Effective anticoagulation regimens are needed to reduce risks of thrombosis and bleeding in animal models of ventricular assist device to verify its hemocompatibility, biologic safety and reliability. This study is to develop a validated anticoagulation procedure for a sheep model to test the newly developed CH-VAD. CH-VAD models were established in six healthy sheep by constructing blood bypass of left ventricle → ventricular assist device → descending aorta. Heparin infusion was used during operation and in the prior 4 days to maintain activated clotting time 1.5-2.0 times the baseline. From the third day, proper dosage of warfarin was used orally to maintain international normalized ratio values within the range of 1.2-2.0. After termination, we examined whether there was thrombosis in the blood pump, grafts, and anastomotic stoma. Macroscopic and histopathologic examinations were performed in major organs to check for congestion and infarction. Bleeding complications were not found in any animals throughout the experiments. Activated clotting time values were 326 ± 33 s intraoperatively and 157 ± 28 s in the prior 4 days postoperatively. Activated partial thromboplastin time values increased slowly and reached the lower limit of the target range on the fourth day. Only in one of six cases was thrombus or fibrosis tissue found in the blood flow channel of the pump. Pathologic analysis showed no thrombosis, necrosis and microembolus in end-stage organs. Under the anticoagulation regimens, coagulation system could be well controlled to avoid thrombosis and bleeding complications in sheep models for CH-VAD.
authors.affiliation: Department of Biomedical Engineering, Beijing Anzhen Hospital, Capital Medical University, Beijing, China.|Department of Biomedical Engineering, Beijing Institute of Heart, Lung, and Blood Vessel Diseases, Beijing, China.
authors.forename: Chuangye
authors.initials: C
authors.lastname: Xu
authors.affiliation: Department of Biomedical Engineering, Beijing Anzhen Hospital, Capital Medical University, Beijing, China.|Department of Biomedical Engineering, Beijing Institute of Heart, Lung, and Blood Vessel Diseases, Beijing, China.
authors.forename: Guanghui
authors.initials: G
authors.lastname: Wu
authors.affiliation: Department of Biomedical Engineering, Beijing Anzhen Hospital, Capital Medical University, Beijing, China.|Department of Biomedical Engineering, Beijing Institute of Heart, Lung, and Blood Vessel Diseases, Beijing, China.
authors.forename: Xiujian
authors.initials: X
authors.lastname: Liu
authors.affiliation: Department of Biomedical Engineering, Beijing Anzhen Hospital, Capital Medical University, Beijing, China.|Department of Biomedical Engineering, Beijing Institute of Heart, Lung, and Blood Vessel Diseases, Beijing, China.
authors.forename: Yuna
authors.initials: Y
authors.lastname: He
authors.affiliation: Cardiac Surgery, Beijing Anzhen Hospital, Capital Medical University, Beijing, China.
authors.forename: Xiaotong
authors.initials: X
authors.lastname: Hou
authors.affiliation: Cardiac Surgery, Beijing Anzhen Hospital, Capital Medical University, Beijing, China.
authors.forename: Haiyang
authors.initials: H
authors.lastname: Li
authors.affiliation: Head Office, ChinaHeart Biomedical Inc., Suzhou, China.
authors.forename: Chen
authors.initials: C
authors.lastname: Chen
authors.affiliation: Head Office, ChinaHeart Biomedical Inc., Suzhou, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Yang
authors.affiliation: Department of Biomedical Engineering, Beijing Anzhen Hospital, Capital Medical University, Beijing, China.|Department of Biomedical Engineering, Beijing Institute of Heart, Lung, and Blood Vessel Diseases, Beijing, China.
authors.forename: Changyan
authors.initials: C
authors.lastname: Lin
mesh_terms: D000818:Animals; D000925:Anticoagulants / Q000494:pharmacology*; D001777:Blood Coagulation / Q000187:drug effects*; D006353:Heart-Assist Devices; D006439:Hemodynamics / Q000187:drug effects; D006493:Heparin / Q000494:pharmacology*; D023421:Models, Animal; D012756:Sheep; D013927:Thrombosis / Q000517:prevention & control; D014859:Warfarin / Q000494:pharmacology*
chemical_list: D000925:Anticoagulants; D014859:Warfarin; D006493:Heparin
keywords: Animal experiment; Anticoagulant; Heparin; Ventricular assist device; Warfarin