title: Programmed Assembly of Host-Guest Protein Crystals.
abstract: The binding and release of guest fluorescent proteins inside a protein crystal with 13 nm axial pores is controlled. Spatially segregated guest protein loading is achieved via sequential binding and release stages. Additionally, selective stabilization of the crystal exterior results in hollow crystalline shells.
authors.affiliation: Chemical and Biological Engineering, Colorado State University, 1301 Campus Delivery, Fort Collins, Colorado, 80523, USA.
authors.forename: Thaddaus R
authors.initials: TR
authors.lastname: Huber
authors.affiliation: Chemical and Biological Engineering, Colorado State University, 1301 Campus Delivery, Fort Collins, Colorado, 80523, USA.
authors.forename: Luke F
authors.initials: LF
authors.lastname: Hartje
authors.affiliation: Chemical and Biological Engineering, Colorado State University, 1301 Campus Delivery, Fort Collins, Colorado, 80523, USA.
authors.forename: Eli C
authors.initials: EC
authors.lastname: McPherson
authors.affiliation: Chemical and Biological Engineering, Colorado State University, 1301 Campus Delivery, Fort Collins, Colorado, 80523, USA.
authors.forename: Ann E
authors.initials: AE
authors.lastname: Kowalski
authors.affiliation: Chemical and Biological Engineering, Colorado State University, 1301 Campus Delivery, Fort Collins, Colorado, 80523, USA.
authors.forename: Christopher D
authors.initials: CD
authors.lastname: Snow
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry*; D016123:Campylobacter jejuni / Q000378:metabolism; D003460:Crystallization; D005456:Fluorescent Dyes / Q000737:chemistry; D013997:Time Factors
chemical_list: D001426:Bacterial Proteins; D005456:Fluorescent Dyes
keywords: host-guest protein crystals; metal affinity capture; nanoporous materials; protein engineering