title: A comparison of microRNA expression profiles from splenic hemangiosarcoma, splenic nodular hyperplasia, and normal spleens of dogs.
abstract: BACKGROUND
  Splenic masses are common in older dogs; yet diagnosis preceding splenectomy and histopathology remains elusive. MicroRNAs (miRNAs) are short, non-coding RNAs that play a role in post-transcriptional regulation, and differential expression of miRNAs between normal and tumor tissue has been used to diagnose neoplastic diseases. The objective of this study was to determine differential expression of miRNAs by use of RNA-sequencing in canine spleens that were histologically confirmed as hemangiosarcoma, nodular hyperplasia, or normal.
  
  
  RESULTS
  Twenty-two miRNAs were found to be differentially expressed in hemangiosarcoma samples (4 between hemangiosarcoma and both nodular hyperplasia and normal spleen and 18 between hemangiosarcoma and normal spleen only). In particular, mir-26a, mir-126, mir-139, mir-140, mir-150, mir-203, mir-424, mir-503, mir-505, mir-542, mir-30e, mir-33b, mir-365, mir-758, mir-22, and mir-452 are of interest in the pathogenesis of hemangiosarcoma.
  
  
  CONCLUSIONS
  Findings of this study confirm the hypothesis that miRNA expression profiles are different between canine splenic hemangiosarcoma, nodular hyperplasia, and normal spleens. A large portion of the differentially expressed miRNAs have roles in angiogenesis, with an additional group of miRNAs being dysregulated in vascular disease processes. Two other miRNAs have been implicated in cancer pathways such as PTEN and cell cycle checkpoints. The finding of multiple miRNAs with roles in angiogenesis and vascular disease is important, as hemangiosarcoma is a tumor of endothelial cells, which are driven by angiogenic stimuli. This study shows that miRNA dysregulation is a potential player in the pathogenesis of canine splenic hemangiosarcoma.
authors.affiliation: Department of Clinical Sciences, Auburn University College of Veterinary Medicine, Auburn University, Auburn, AL, USA. jgrimes@uga.edu.|Department of Small Animal Medicine and Surgery, College of Veterinary Medicine, University of Georgia, 2200 College Station Road, Athens, GA, 30602, USA. jgrimes@uga.edu.
authors.forename: Janet A
authors.initials: JA
authors.lastname: Grimes
authors.affiliation: Genomics Services Laboratory, HudsonAlpha Institute for Biotechnology, Huntsville, AL, USA.
authors.forename: Nripesh
authors.initials: N
authors.lastname: Prasad
authors.affiliation: Genomics Services Laboratory, HudsonAlpha Institute for Biotechnology, Huntsville, AL, USA.
authors.forename: Shawn
authors.initials: S
authors.lastname: Levy
authors.affiliation: Department of Pathobiology, Auburn University College of Veterinary Medicine, Auburn University, Auburn, AL, USA.
authors.forename: Russell
authors.initials: R
authors.lastname: Cattley
authors.affiliation: Department of Clinical Sciences, Auburn University College of Veterinary Medicine, Auburn University, Auburn, AL, USA.
authors.forename: Stephanie
authors.initials: S
authors.lastname: Lindley
authors.affiliation: Department of Clinical Sciences, Auburn University College of Veterinary Medicine, Auburn University, Auburn, AL, USA.
authors.forename: Harry W
authors.initials: HW
authors.lastname: Boothe
authors.affiliation: Department of Clinical Sciences, Auburn University College of Veterinary Medicine, Auburn University, Auburn, AL, USA.
authors.forename: Ralph A
authors.initials: RA
authors.lastname: Henderson
authors.affiliation: Scott Ritchey Research Center, Auburn University College of Veterinary Medicine, Auburn University, Auburn, AL, USA.
authors.forename: Bruce F
authors.initials: BF
authors.lastname: Smith
mesh_terms: D000818:Animals; D004283:Dog Diseases / Q000175:diagnosis / Q000235:genetics* / Q000473:pathology; D004285:Dogs; D020869:Gene Expression Profiling; D006394:Hemangiosarcoma / Q000175:diagnosis / Q000235:genetics / Q000473:pathology / Q000662:veterinary*; D006965:Hyperplasia / Q000175:diagnosis / Q000235:genetics / Q000662:veterinary; D035683:MicroRNAs / Q000096:biosynthesis*; D013154:Spleen / Q000378:metabolism* / Q000473:pathology*; D013160:Splenic Neoplasms / Q000175:diagnosis / Q000235:genetics / Q000662:veterinary*
chemical_list: D035683:MicroRNAs
keywords: Angiogenesis; Canine; Hemangiosarcoma; MicroRNA; RNA-sequencing; Splenic mass