title: Single-nucleotide polymorphisms of MMP2 in MMP/TIMP pathways associated with the risk of alcohol-induced osteonecrosis of the femoral head in Chinese males: A case-control study.
abstract: The proportion of alcohol-induced osteonecrosis of the femoral head (ONFH) in all ONFH patients was 30.7%, with males prevailing among the ONFH patients in mainland China (70.1%). Matrix metalloproteinase 2 (MMP2), a member of the MMP gene family, encodes the enzyme MMP2, which can promote osteoclast migration, attachment, and bone matrix degradation. In this case-control study, we aimed to investigate the association between MMP2 and the alcohol-induced ONFH in Chinese males.In total, 299 patients with alcohol-induced ONFH and 396 healthy controls were recruited for a case-control association study. Five single-nucleotide polymorphisms within the MMP2 locus were genotyped and examined for their correlation with the risk of alcohol-induced ONFH and treatment response using Pearson χ test and unconditional logistic regression analysis. We identified 3 risk alleles for carriers: the allele "T" of rs243849 increased the risk of alcohol-induced ONFH in the allele model, the log-additive model without adjustment, and the log-additive model with adjustment for age. Conversely, the genotypes "CC" in rs7201 and "CC" in rs243832 decreased the risk of alcohol-induced ONFH, as revealed by the recessive model. After the Bonferroni multiple adjustment, no significant association was found. Furthermore, the haplotype analysis showed that the "TT" haplotype of MMP2 was more frequent among patients with alcohol-induced ONFH by unconditional logistic regression analysis adjusted for age.In conclusion, there may be an association between MMP2 and the risk of alcohol-induced ONFH in North-Chinese males. However, studies on larger populations are needed to confirm this hypothesis; these data may provide a theoretical foundation for future studies.
authors.affiliation: Clinical Laboratory of Hong-Hui Hospital, Xi'an Jiaotong University College of Medicine Key Laboratory of Resource Biology and Biotechnology in Western China (Northwest University), Ministry of Education, College of Life Sciences, Northwest University Xi'an Tiangen Precision Medical Institute Institution of Basic Medical Science, Xi'an Medical University, Xi'an, Shaanxi Province, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Yu
authors.forename: Zhilan
authors.initials: Z
authors.lastname: Xie
authors.forename: Jihan
authors.initials: J
authors.lastname: Wang
authors.forename: Chu
authors.initials: C
authors.lastname: Chen
authors.forename: Shuli
authors.initials: S
authors.lastname: Du
authors.forename: Peng
authors.initials: P
authors.lastname: Chen
authors.forename: Bin
authors.initials: B
authors.lastname: Li
authors.forename: Tianbo
authors.initials: T
authors.lastname: Jin
authors.forename: Heping
authors.initials: H
authors.lastname: Zhao
mesh_terms: D000328:Adult; D017677:Age Distribution; D000428:Alcohol Drinking / Q000009:adverse effects*; D016022:Case-Control Studies; D002681:China / Q000453:epidemiology; D005271:Femur Head Necrosis / Q000453:epidemiology / Q000209:etiology / Q000235:genetics*; D005786:Gene Expression Regulation; D005819:Genetic Markers; D020022:Genetic Predisposition to Disease / Q000453:epidemiology*; D006801:Humans; D015994:Incidence; D016015:Logistic Models; D008297:Male; D020778:Matrix Metalloproteinase 2 / Q000235:genetics*; D008875:Middle Aged; D016017:Odds Ratio; D020641:Polymorphism, Single Nucleotide; D012016:Reference Values; D018570:Risk Assessment
chemical_list: D005819:Genetic Markers; D020778:Matrix Metalloproteinase 2