title: Comparing lamin proteins post-translational relative stability using a 2A peptide-based system reveals elevated resistance of progerin to cellular degradation.
abstract: Nuclear lamins are the major components of the nuclear lamina at the periphery of the nucleus, supporting the nuclear envelope and participating in many nuclear processes, including DNA replication, transcription and chromatin organization. A group of diseases, the laminopathies, is associated with mutations in lamin genes. One of the most striking cases is Hutchinson-Gilford progeria syndrome (HGPS) which is the consequence of a lamin A dominant negative mutant named progerin. Due to the abnormal presence of a permanent C-terminal farnesyl tail, progerin gradually accumulates on the nuclear membrane, perturbing a diversity of signalings and transcriptional events. The accumulation of progerin has led to the speculation that progerin possesses higher stability than the wild type lamin A protein. However, the low solubility of lamin proteins renders traditional immunoprecipitation-dependent methods such as pulse-chase analysis ineffective for comparing the relative stabilities of mutant and wild type lamins. Here, we employ a novel platform for inferring differences in lamin stability, which is based on normalization to a co-translated reporter protein following porcine teschovirus-1 2A peptide-mediated co-translational cleavage. The results obtained using this method support the notion that progerin is more stable than lamin A. Moreover, treatment of FTI reduces progerin relative stability to the level of wild type lamin A.
authors.affiliation: a Department of Cell Biology and Molecular Genetics , University of Maryland College Park , MD , USA.
authors.forename: Di
authors.initials: D
authors.lastname: Wu
authors.affiliation: b The Department of Biochemistry and Molecular Biology , Oregon Health & Science University , Portland , OR , USA.
authors.forename: Phillip A
authors.initials: PA
authors.lastname: Yates
authors.affiliation: a Department of Cell Biology and Molecular Genetics , University of Maryland College Park , MD , USA.
authors.forename: Haoyue
authors.initials: H
authors.lastname: Zhang
authors.affiliation: a Department of Cell Biology and Molecular Genetics , University of Maryland College Park , MD , USA.
authors.forename: Kan
authors.initials: K
authors.lastname: Cao
mesh_terms: D000595:Amino Acid Sequence; D005347:Fibroblasts / Q000378:metabolism; D006801:Humans; D034904:Lamin Type A / Q000737:chemistry* / Q000378:metabolism*; D059630:Mesenchymal Stem Cells / Q000378:metabolism; D010455:Peptides / Q000378:metabolism*; D014176:Protein Biosynthesis; D055550:Protein Stability; D059748:Proteolysis
chemical_list: D034904:Lamin Type A; D010455:Peptides; C090764:prelamin A
keywords: FTI; lamin A; lamin B1; progerin; protein relative abundance; protein relative stability