title: Production of α-1,4-glucosidase from Bacillus licheniformis KIBGE-IB4 by utilizing sweet potato peel.
abstract: In the current study, sweet potato peel (Ipomoea batatas) was observed as the most favorable substrate for the maximum synthesis of α-1,4-glucosidase among various agro-industrial residues. Bacillus licheniformis KIBGE-IB4 produced 6533.0 U ml-1of α-1,4-glucosidase when growth medium was supplemented with 1% dried and crushed sweet potato peel. It was evident from the results that bacterial isolate secreted 6539.0 U ml-1of α-1,4-glucosidase in the presence of 0.4% peptone and meat extract with 0.1% yeast extract. B. licheniformis KIBGE-IB4 released 6739.0 and 7190.0 U ml-1of enzyme at 40 °C and pH 7.0, respectively. An improved and cost-effective growth medium design resulted 8590.0 U ml-1of α-1,4-glucosidase with 1.3-fold increase as compared to initial amount from B. licheniformis KIBGE-IB4. This enzyme can be used to fulfill the accelerating demand of food and pharmaceutical industries. Further purification and immobilization of this enzyme can also enhance its utility for various commercial applications. Graphical abstract Pictorial representation of maltase production from sweet potato peel.
authors.affiliation: Department of Biotechnology, Shaheed Benazir Bhutto University, Sheringal, Dir Upper, KPK, Pakistan. asif_biotech33@sbbu.edu.pk.|The Karachi Institute of Biotechnology and Genetic Engineering (KIBGE), University of Karachi, Karachi, 75270, Pakistan. asif_biotech33@sbbu.edu.pk.
authors.forename: Muhammad Asif
authors.initials: MA
authors.lastname: Nawaz
authors.affiliation: Department of Biotechnology, Federal Urdu University of Arts, Science, and Technology, Karachi, Pakistan. zainabb.bibi@gmail.com.
authors.forename: Zainab
authors.initials: Z
authors.lastname: Bibi
authors.affiliation: National Institute of Biotechnology and Genetic Engineering (NIBGE), Faisalabad, Pakistan.
authors.forename: Asad
authors.initials: A
authors.lastname: Karim
authors.affiliation: Department of Chemistry, University of Turbat, Kech, Balochistan, Pakistan.
authors.forename: Haneef Ur
authors.initials: HU
authors.lastname: Rehman
authors.affiliation: Department of Microbiology, Abdul Wali Khan University, Garden Campus, Mardan, Pakistan.
authors.forename: Muhsin
authors.initials: M
authors.lastname: Jamal
authors.affiliation: Department of Botany, University of Malakand, Chaldara, KPK, Pakistan.
authors.forename: Tour
authors.initials: T
authors.lastname: Jan
authors.affiliation: The Karachi Institute of Biotechnology and Genetic Engineering (KIBGE), University of Karachi, Karachi, 75270, Pakistan.
authors.forename: Afsheen
authors.initials: A
authors.lastname: Aman
authors.affiliation: The Karachi Institute of Biotechnology and Genetic Engineering (KIBGE), University of Karachi, Karachi, 75270, Pakistan.
authors.forename: Shah Ali Ul
authors.initials: SA
authors.lastname: Qader
mesh_terms: D000069976:Bacillus licheniformis / Q000201:enzymology*; D002244:Carbon / Q000378:metabolism; D003470:Culture Media; D005959:Glucosidases / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D027723:Ipomoea batatas / Q000378:metabolism*; D013696:Temperature
chemical_list: D003470:Culture Media; D002244:Carbon; D005959:Glucosidases
keywords: Agro-industrial waste; Biodegradation; Fermentation; Industrial applications; Maltase