title: Distinct cold responsiveness of a StInvInh2 gene promoter in transgenic potato tubers with contrasting resistance to cold-induced sweetening.
abstract: Potato (Solanum tuberosum L.) vacuolar invertase (β-fructofuranosidase; EC 3.2.1.26) inhibitor 2 (StInvInh2) plays an important role in cold-induced sweetening (CIS) of potato tubers. The transcript levels of StInvInh2 were increased by prolonged cold in potato tubers with CIS-resistance but decreased in potato tubers with CIS-sensitivity. However, the transcript regulation mechanisms of StInvInh2 responding to prolonged cold are largely unclear in CIS-resistant and CIS-sensitive genotypes. In the present study, the 5'-flanking sequence of the StInvInh2 was cloned, and cis-acting elements were predicted. No informative differences in StInvInh2 promoter structure between resistant and sensitive-CIS potato genotypes were observed. Histochemical assay showed that the promoter of StInvInh2 mainly governed β-glucuronidase (GUS) expression in potato microtubers. Quantitative analysis of GUS expression suggested that StInvInh2 promoter activity was enhanced by prolonged cold in CIS-resistant genotype tubers but suppressed in CIS-sensitive tubers. These findings provide essential information regarding transcriptional regulatory mechanisms of StInvInh2 in cold-stored tubers contrasting CIS capacity.
authors.affiliation: Key Open Laboratory of Southwest Crop Genetic Improvement and Breeding, Ministry of Agriculture, Southwest University, Chongqing 400075, China; Engineering Research Center of South Upland Agriculture, Ministry of Education, Southwest University, Chongqing 400075, China. Electronic address: liuxun828@swu.edu.cn.
authors.forename: Xun
authors.initials: X
authors.lastname: Liu
authors.affiliation: Key Open Laboratory of Southwest Crop Genetic Improvement and Breeding, Ministry of Agriculture, Southwest University, Chongqing 400075, China; Engineering Research Center of South Upland Agriculture, Ministry of Education, Southwest University, Chongqing 400075, China.
authors.forename: Weiling
authors.initials: W
authors.lastname: Shi
authors.affiliation: Key Open Laboratory of Southwest Crop Genetic Improvement and Breeding, Ministry of Agriculture, Southwest University, Chongqing 400075, China; Engineering Research Center of South Upland Agriculture, Ministry of Education, Southwest University, Chongqing 400075, China.
authors.forename: Wang
authors.initials: W
authors.lastname: Yin
authors.affiliation: Key Open Laboratory of Southwest Crop Genetic Improvement and Breeding, Ministry of Agriculture, Southwest University, Chongqing 400075, China; Engineering Research Center of South Upland Agriculture, Ministry of Education, Southwest University, Chongqing 400075, China. Electronic address: wjchun@swu.edu.cn.
authors.forename: Jichun
authors.initials: J
authors.lastname: Wang
mesh_terms: D024506:5' Flanking Region / Q000235:genetics; D001483:Base Sequence; D003080:Cold Temperature; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D005838:Genotype; D005966:Glucuronidase / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D035281:Plant Tubers / Q000201:enzymology / Q000235:genetics*; D030821:Plants, Genetically Modified; D011401:Promoter Regions, Genetic; D011198:Solanum tuberosum / Q000201:enzymology / Q000235:genetics*; D013649:Taste; D043324:beta-Fructofuranosidase / Q000235:genetics* / Q000378:metabolism
chemical_list: D010940:Plant Proteins; D043324:beta-Fructofuranosidase; D005966:Glucuronidase
keywords: Cold response; Potato; StInvInh2 promoter; Transcriptional regulation