title: Aniline Induces Oxidative Stress and Apoptosis of Primary Cultured Hepatocytes.
abstract: The toxicity and carcinogenicity of aniline in humans and animals have been well documented. However, the molecular mechanism involved in aniline-induced liver toxicity and carcinogenesis remains unclear. In our research, primary cultured hepatocytes were exposed to aniline (0, 1.25, 2.50, 5.0 and 10.0 μg/mL) for 24 h in the presence or absence ofN-acetyl-l-cysteine (NAC). Levels of reactive oxygen species (ROS), malondialdehyde (MDA), and glutathione (GSH), activities of superoxide dismutase (SOD) and catalase (CAT), mitochondrial membrane potential, DNA damage, cell viability, and apoptosis were detected. Levels of ROS and MDA were significantly increased and levels of GSH and CAT, activity of SOD, and mitochondrial membrane potential in hepatocytes were significantly decreased by aniline compared with the negative control group. The tail moment and DNA content of the tail in exposed groups were significantly higher than those in the negative control group. Cell viability was reduced and apoptotic death was induced by aniline in a concentration-dependent manner. The phenomena of ROS generation, oxidative damage, loss of mitochondrial membrane potential, DNA damage and apoptosis could be prevented if ROS inhibitor NAC was added. ROS generation is involved in the loss of mitochondrial membrane potential and DNA injury, which may play a role in aniline-induced apoptosis in hepatocytes. Our study provides insight into the mechanism of aniline-induced toxicity and apoptosis of hepatocytes.
authors.affiliation: Department of Occupational Health, College of Public Health, Harbin Medical University, Harbin 150086, China. wangyue5056@163.com.
authors.forename: Yue
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Sanitary Microorganisms, College of Public Health, Harbin Medical University, Harbin 150086, China. m15114515383@163.com.
authors.forename: Hong
authors.initials: H
authors.lastname: Gao
authors.affiliation: Department of Environmental Health, College of Public Health, Harbin Medical University, Harbin 150086, China. jljz5858@163.com.
authors.forename: Xiao-Lin
authors.initials: XL
authors.lastname: Na
authors.affiliation: Department of Environmental Health, College of Public Health, Harbin Medical University, Harbin 150086, China. dong.shuying@163.com.
authors.forename: Shu-Ying
authors.initials: SY
authors.lastname: Dong
authors.affiliation: Department of Occupational Health, College of Public Health, Harbin Medical University, Harbin 150086, China. donghongwei0824@163.com.
authors.forename: Hong-Wei
authors.initials: HW
authors.lastname: Dong
authors.affiliation: Department of Environmental Health, College of Public Health, Harbin Medical University, Harbin 150086, China. jiali1013@126.com.
authors.forename: Jia
authors.initials: J
authors.lastname: Yu
authors.affiliation: Department of Environmental Health, College of Public Health, Harbin Medical University, Harbin 150086, China. jiali1013@163.com.
authors.forename: Li
authors.initials: L
authors.lastname: Jia
authors.affiliation: Department of Occupational Health, College of Public Health, Harbin Medical University, Harbin 150086, China. wuyonghui777@163.com.
authors.forename: Yong-Hui
authors.initials: YH
authors.lastname: Wu
mesh_terms: D000111:Acetylcysteine; D000814:Aniline Compounds / Q000633:toxicity*; D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D002374:Catalase / Q000378:metabolism; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D004785:Environmental Pollutants / Q000633:toxicity*; D005978:Glutathione / Q000378:metabolism; D022781:Hepatocytes / Q000187:drug effects* / Q000378:metabolism; D006801:Humans; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D053078:Membrane Potential, Mitochondrial; D010084:Oxidation-Reduction; D018384:Oxidative Stress / Q000187:drug effects*; D051381:Rats; D017207:Rats, Sprague-Dawley; D017382:Reactive Oxygen Species / Q000378:metabolism; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D000814:Aniline Compounds; D004785:Environmental Pollutants; D017382:Reactive Oxygen Species; D008315:Malondialdehyde; D002374:Catalase; D013482:Superoxide Dismutase; D005978:Glutathione; C023650:aniline; D000111:Acetylcysteine
keywords: aniline; apoptosis; hepatocytes; reactive oxygen species