title: Bioassay-Guided Isolation of Cytotoxic Isocryptoporic Acids from Cryptoporus volvatus.
abstract: The present work constitutes a contribution to the phytochemical investigation ofCryptoporus volvatusaiming to search for effective cytotoxic constituents against tumor cell lines in vivo. Bioassay-guided separation of the ethylacetate extract ofC. volvatusafforded four new isocryptoporic acid (ICA) derivatives, ICA-B trimethyl ester (1), ICA-E (2), ICA-E pentamethyl ester (3), and ICA-G (4), together with nine known cryptoporic acids. These isocryptoporic acids are isomers of the cryptoporic acids with drimenol instead of albicanol as the terpenoid fragment; their structures were elucidated on the basis of spectroscopic evidences (UV, IR, HRMS, and NMR) and comparison with literature values. All isolates show certain cytotoxic activities against five tumor cell lines. Among them, compound4showed an comparable activity to that of the positive controlcis-platin, while other compounds exhibited weak cytotoxic activities.
authors.affiliation: Key Laboratory for Forest Resources Conservation and Use in the Southwest Mountains of China, Southwest Forestry University, Kunming 650224, China. zly4321@sina.com.|School of Pharmacy, Wannan Medical College, Wuhu 241002, China. zly4321@sina.com.
authors.forename: Ling-Yun
authors.initials: LY
authors.lastname: Zhou
authors.affiliation: Key Laboratory for Forest Resources Conservation and Use in the Southwest Mountains of China, Southwest Forestry University, Kunming 650224, China. 13187815521@163.com.
authors.forename: Xiao-Hong
authors.initials: XH
authors.lastname: Yu
authors.affiliation: Key Laboratory for Forest Resources Conservation and Use in the Southwest Mountains of China, Southwest Forestry University, Kunming 650224, China. nongxue022@163.com.
authors.forename: Bin
authors.initials: B
authors.lastname: Lu
authors.affiliation: Key Laboratory for Forest Resources Conservation and Use in the Southwest Mountains of China, Southwest Forestry University, Kunming 650224, China. huayan1216@163.com.
authors.forename: Yan
authors.initials: Y
authors.lastname: Hua
mesh_terms: D001681:Biological Assay; D045744:Cell Line, Tumor; D004354:Drug Screening Assays, Antitumor; D006801:Humans; D020072:Polyporales / Q000737:chemistry*; D012717:Sesquiterpenes / Q000302:isolation & purification*; D013057:Spectrum Analysis / Q000379:methods
chemical_list: D012717:Sesquiterpenes
keywords: Cryptoporus volvatus; cytotoxic activities against cancer cell lines; isocryptoporic acids