title: Evaluation of interactions between RAW264.7 macrophages and small molecules by capillary electrophoresis.
abstract: In this study, the affinity interactions between RAW 264.7 macrophages and three small molecules including naringin, oleuropein and paeoniflorin were evaluated by affinity capillary electrophoresis (ACE), partial filling affinity capillary electrophoresis (PFACE) and frontal analysis capillary electrophoresis (FACE), respectively. The result indicated that ACE (varying concentrations of cell suspension were filled in the capillary as receptor) may not be suitable for the evaluation of interactions between cell and small molecules due to the high viscosity of cell suspension; PFACE can qualitatively evaluate the interaction, but the difference in viscosity between RAW264.7 suspension and buffer effects on the liner relationship between filling length and injection time, which makes the calculation of binding constant difficult. Furthermore, based on the PFACE results, naringin showed stronger interaction with macrophages than the other two molecules; taking advantage of the aggregation phenomenon of cell induced by electric field, FACE was successfully used to determine the stoichiometry (n = 5×109) and binding constant (Kb= 1×104L/mol) of the interaction between RAW264.7 and naringin.
authors.affiliation: School of Chemistry and Chemical Engineering, Chongqing University, Chongqing, P. R., China.
authors.forename: Feng-Qin
authors.initials: FQ
authors.lastname: Wang
authors.affiliation: School of Chemistry and Chemical Engineering, Chongqing University, Chongqing, P. R., China.
authors.forename: Qiao-Qiao
authors.initials: QQ
authors.lastname: Li
authors.affiliation: School of Chemistry and Chemical Engineering, Chongqing University, Chongqing, P. R., China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: School of Chemistry and Chemical Engineering, Chongqing University, Chongqing, P. R., China.
authors.forename: Yin-Zhen
authors.initials: YZ
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao, P. R., China.
authors.forename: Yuan-Jia
authors.initials: YJ
authors.lastname: Hu
authors.affiliation: State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao, P. R., China.
authors.forename: Peng
authors.initials: P
authors.lastname: Li
authors.affiliation: State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao, P. R., China.
authors.forename: Jian-Bo
authors.initials: JB
authors.lastname: Wan
authors.affiliation: School of Chemistry and Chemical Engineering, Chongqing University, Chongqing, P. R., China.
authors.forename: Feng-Qing
authors.initials: FQ
authors.lastname: Yang
authors.affiliation: School of Chemistry and Chemical Engineering, Chongqing University, Chongqing, P. R., China.
authors.forename: Zhi-Ning
authors.initials: ZN
authors.lastname: Xia
mesh_terms: D000818:Animals; D002021:Buffers; D002460:Cell Line; D004560:Electricity; D019075:Electrophoresis, Capillary / Q000379:methods*; D044950:Flavanones / Q000032:analysis*; D005960:Glucosides / Q000032:analysis*; D057888:Iridoid Glucosides; D039823:Iridoids / Q000032:analysis*; D008264:Macrophages / Q000737:chemistry*; D051379:Mice; D039821:Monoterpenes / Q000032:analysis*; D006027:Glycosides
chemical_list: D002021:Buffers; D044950:Flavanones; D005960:Glucosides; D057888:Iridoid Glucosides; D039823:Iridoids; D039821:Monoterpenes; C015423:peoniflorin; D006027:Glycosides; C002769:oleuropein; C005274:naringin
keywords: Affinity interaction; Cell aggregation; Frontal analysis capillary electrophoresis; RAW264.7 macrophage