title: High-resolution transcript profiling reveals shoot abscission process of spruce dwarf mistletoe Arceuthobium sichuanense in response to ethephon.
abstract: Arceuthobium (dwarf mistletoes) are hemiparasites that may cause great damage to infected trees belonging to Pinaceae and Cupressaceae. Currently, dwarf mistletoe control involves the use of the ethylene-producing product ethephon (ETH), which acts by inducing dwarf mistletoe shoot abscission. However, the process by which ETH functions is mostly unknown. Therefore, the transcriptome of the ETH-exposed plants was compared to non-exposed controls to identify genes associated with the response to ethephon. In this study, the reference transcriptome was contained 120,316 annotated unigenes, with a total of 21,764 ETH-responsive differentially expressed unigenes were identified. These ETH-associated genes clustered into 20 distinctly expressed pattern groups, providing a view of molecular events with good spatial and temporal resolution. As expected, the greatest number of unigenes with changed expression were observed at the onset of abscission, suggesting induction by ethylene. ETH also affected genes associated with shoot abscission processes including hormone biosynthesis and signaling, cell wall hydrolysis and modification, lipid transference, and more. The comprehensive transcriptome data set provides a wealth of genomic resources for dwarf mistletoe communities and contributes to a better understanding of the molecular regulatory mechanism of ethylene-caused shoots abscission.
authors.affiliation: The Key Laboratory for Silviculture and Conservation of Ministry of Education, College of Forestry, Beijing Forestry University, Beijing, China.
authors.forename: Yonglin
authors.initials: Y
authors.lastname: Wang
authors.affiliation: The Key Laboratory for Silviculture and Conservation of Ministry of Education, College of Forestry, Beijing Forestry University, Beijing, China.
authors.forename: Dianguang
authors.initials: D
authors.lastname: Xiong
authors.affiliation: The Key Laboratory for Silviculture and Conservation of Ministry of Education, College of Forestry, Beijing Forestry University, Beijing, China.
authors.forename: Ning
authors.initials: N
authors.lastname: Jiang
authors.affiliation: The Key Laboratory for Silviculture and Conservation of Ministry of Education, College of Forestry, Beijing Forestry University, Beijing, China.|Academy of Forest Inventory and Planning, State Forestry Administration, Beijing, China.
authors.forename: Xuewu
authors.initials: X
authors.lastname: Li
authors.affiliation: Forest Pest Control and Quarantine Station of Qinghai Province, Xining, China.
authors.forename: Qiqing
authors.initials: Q
authors.lastname: Yang
authors.affiliation: The Key Laboratory for Silviculture and Conservation of Ministry of Education, College of Forestry, Beijing Forestry University, Beijing, China.
authors.forename: Chengming
authors.initials: C
authors.lastname: Tian
mesh_terms: D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant / Q000187:drug effects*; D063990:Gene Ontology; D017343:Genes, Plant; D006540:Herbicides / Q000494:pharmacology*; D009943:Organophosphorus Compounds / Q000494:pharmacology*; D018520:Plant Shoots / Q000187:drug effects / Q000378:metabolism; D018749:RNA, Plant / Q000096:biosynthesis / Q000235:genetics; D013997:Time Factors; D059467:Transcriptome / Q000187:drug effects*; D028163:Viscaceae / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism
chemical_list: D006540:Herbicides; D009943:Organophosphorus Compounds; D018749:RNA, Plant; C005073:ethephon