title: A comparative assessment of cytotoxicity of commonly used agricultural insecticides to human and insect cells.
abstract: The cytotoxic potential of 13 commonly used agricultural insecticides was examined using cell-based systems with three human HepG2, Hek293, HeLa cells and three insect Tn5B1-4, Sf-21, and Drosophila S2 cells. Data showed that (1) an enhancement of some insecticides (e.g. pyrethroids) on cells proliferation; (2) an inhibition of some insecticides on cells viability; (3) various levels of susceptibility of different cells to the same insecticide; and (4) the cell type dependent sensitivity to different insecticides. The degree of cytotoxicity of insecticides on human cells was significantly lower than that on insect cells (P<0.05). Methomyl, even 20μg/ml, showed little cytotoxicity at 24h exposure whereas emamectin benzoate possessed the strongest cytotoxic potential in a dose-dependent fashion. The results revealed comparable cytotoxic property of agricultural insecticides against intact cells.
authors.affiliation: Shanghai Key Lab of Chemical Biology, School of Pharmacy, East China University of Science and Technology, Shanghai 200237, PR China.
authors.forename: Xinming
authors.initials: X
authors.lastname: Yun
authors.affiliation: Shanghai Key Lab of Chemical Biology, School of Pharmacy, East China University of Science and Technology, Shanghai 200237, PR China. Electronic address: qchuang@ecust.edu.cn.
authors.forename: Qingchun
authors.initials: Q
authors.lastname: Huang
authors.affiliation: Shanghai Key Lab of Chemical Biology, School of Pharmacy, East China University of Science and Technology, Shanghai 200237, PR China.
authors.forename: Wenbing
authors.initials: W
authors.lastname: Rao
authors.affiliation: State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, PR China.
authors.forename: Ciying
authors.initials: C
authors.lastname: Xiao
authors.affiliation: Shanghai Key Lab of Chemical Biology, School of Pharmacy, East China University of Science and Technology, Shanghai 200237, PR China.
authors.forename: Tao
authors.initials: T
authors.lastname: Zhang
authors.affiliation: Shanghai Key Lab of Chemical Biology, School of Pharmacy, East China University of Science and Technology, Shanghai 200237, PR China.
authors.forename: Zhifan
authors.initials: Z
authors.lastname: Mao
authors.affiliation: Shanghai Key Lab of Chemical Biology, School of Pharmacy, East China University of Science and Technology, Shanghai 200237, PR China.
authors.forename: Ziyi
authors.initials: Z
authors.lastname: Wan
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D003603:Cytotoxins / Q000633:toxicity*; D057809:HEK293 Cells; D006367:HeLa Cells; D006801:Humans; D007313:Insecta / Q000187:drug effects*; D007306:Insecticides / Q000633:toxicity*; D007559:Ivermectin / Q000031:analogs & derivatives / Q000633:toxicity; D008724:Methomyl / Q000633:toxicity; D011722:Pyrethrins / Q000633:toxicity
chemical_list: D003603:Cytotoxins; D007306:Insecticides; D011722:Pyrethrins; D008724:Methomyl; D007559:Ivermectin; C108024:emamectin benzoate
keywords: Cell-based systems; Cytotoxicity; Human cells; Insect cells; Insecticides