title: Highly Efficient Intramolecular Electrochemiluminescence Energy Transfer for Ultrasensitive Bioanalysis of Aflatoxin M1.
abstract: The intermolecular electrochemiluminescence resonance energy transfer (ECL-RET) between luminol and Ru(bpy)32+was studied extensively to achieve the sensitive bioanalysis owing to the perfect spectral overlap of the donor and acceptor, but it still suffers from the challenging issue of low energy-transfer efficiency. The intramolecular ECL-RET towards the novel ECL compound containing the donor of luminol and the acceptor of Ru(bpy)2(mcpbpy)2+(Lum-Ru) was designed and investigated. With the high-efficient ECL-RET in one molecule, the highly intense ECL signal of Lum-Ru was obtained owing to the short path of energy transmission and less energy loss between luminol and Ru(bpy)2(mcpbpy)2+. Lum-Ru was further applied to construct a signal-off electrochemiluminescence (ECL) aptasensor for ultrasensitive detection of a harsh carcinogen of Aflatoxin M1 (AFM1). This sensing platform also provides a significant boost for the trace detection of other biomolecules in clinical analysis.
authors.affiliation: Key Laboratory of Luminescent and Real-Time Analytical Chemistry (Southwest University), Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Chongqing, 400715, P. R. China.
authors.forename: Jia-Li
authors.initials: JL
authors.lastname: Liu
authors.affiliation: Key Laboratory of Luminescent and Real-Time Analytical Chemistry (Southwest University), Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Chongqing, 400715, P. R. China.
authors.forename: Min
authors.initials: M
authors.lastname: Zhao
authors.affiliation: Key Laboratory of Luminescent and Real-Time Analytical Chemistry (Southwest University), Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Chongqing, 400715, P. R. China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhuo
authors.affiliation: Key Laboratory of Luminescent and Real-Time Analytical Chemistry (Southwest University), Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Chongqing, 400715, P. R. China.
authors.forename: Ya-Qin
authors.initials: YQ
authors.lastname: Chai
authors.affiliation: Key Laboratory of Luminescent and Real-Time Analytical Chemistry (Southwest University), Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Chongqing, 400715, P. R. China.
authors.forename: Ruo
authors.initials: R
authors.lastname: Yuan
mesh_terms: D015082:2,2'-Dipyridyl / Q000737:chemistry; D016607:Aflatoxin M1 / Q000032:analysis*; D056831:Coordination Complexes / Q000737:chemistry; D004247:DNA / Q000737:chemistry; D058266:Dielectric Spectroscopy; D055664:Electrochemical Techniques; D004735:Energy Transfer; D005296:Ferrous Compounds / Q000737:chemistry; D008163:Luminescent Measurements; D008165:Luminol / Q000737:chemistry; D000075163:Metallocenes / Q000737:chemistry; D015203:Reproducibility of Results; D012428:Ruthenium / Q000737:chemistry; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D056831:Coordination Complexes; D005296:Ferrous Compounds; D000075163:Metallocenes; D015082:2,2'-Dipyridyl; D008165:Luminol; D016607:Aflatoxin M1; D012428:Ruthenium; D004247:DNA; C004998:ferrocene
keywords: Aflatoxin M1; electrochemiluminescence; resonance energy transfer; ruthenium; target-induced circular exponential amplification