title: Use of low density polyethylene membranes for assessment of genotoxicity of PAHs in the Seine River.
abstract: The genotoxicity of river water dissolved contaminants is usually estimated after grab sampling of river water. Water contamination can now be obtained with passive samplers that allow a time-integrated sampling of contaminants. Since it was verified that low density polyethylene membranes (LDPE) accumulate labile hydrophobic compounds, their use was proposed as a passive sampler. This study was designed to test the applicability of passive sampling for combined chemical and genotoxicity measurements. The LDPE extracts were tested with the umu test (TA1535/pSK1002 ± S9) and the Ames assay (TA98, TA100 and YG1041 ± S9). We describe here this new protocol and its application in two field studies on four sites of the Seine River. Field LDPE extracts were negative with the YG1041 and TA100 and weakly positive with the TA98 + S9 and Umu test. Concentrations of labile mutagenic PAHs were higher upstream of Paris than downstream of Paris. Improvement of the method is needed to determine the genotoxicity of low concentrations of labile dissolved organic contaminants.
authors.affiliation: IRSTEA, UR Hydrosystèmes et Bioprocédés, 1 rue Pierre-Gilles de Gennes, CS10030, 92761, Antony cedex, France. francoise.vincent@ifremer.fr.|Present address: IFREMER, Laboratoire de Microbiologie - LNR, rue de l'île d'Yeu, BP 21105, F 44311, Nantes, Cedex 03, France. francoise.vincent@ifremer.fr.
authors.forename: Françoise
authors.initials: F
authors.lastname: Vincent-Hubert
authors.affiliation: IRSTEA, UR Hydrosystèmes et Bioprocédés, 1 rue Pierre-Gilles de Gennes, CS10030, 92761, Antony cedex, France.
authors.forename: Emmanuelle
authors.initials: E
authors.lastname: Uher
authors.affiliation: Biogénotoxicologie et Mutagenèse Environnementales (EA 1784), Université de la Méditerranée, Marseille, France.
authors.forename: Carole
authors.initials: C
authors.lastname: Di Giorgio
authors.affiliation: IRSTEA, UR Hydrosystèmes et Bioprocédés, 1 rue Pierre-Gilles de Gennes, CS10030, 92761, Antony cedex, France.
authors.forename: Cécile
authors.initials: C
authors.lastname: Michel
authors.affiliation: Biogénotoxicologie et Mutagenèse Environnementales (EA 1784), Université de la Méditerranée, Marseille, France.
authors.forename: Michel
authors.initials: M
authors.lastname: De Meo
authors.affiliation: IRSTEA, UR Hydrosystèmes et Bioprocédés, 1 rue Pierre-Gilles de Gennes, CS10030, 92761, Antony cedex, France.
authors.forename: Catherine
authors.initials: C
authors.lastname: Gourlay-France
mesh_terms: D004249:DNA Damage / Q000187:drug effects*; D004784:Environmental Monitoring / Q000295:instrumentation / Q000379:methods; D005602:France; D008567:Membranes, Artificial; D009152:Mutagenicity Tests / Q000295:instrumentation*; D011084:Polycyclic Aromatic Hydrocarbons / Q000032:analysis / Q000633:toxicity*; D020959:Polyethylene / Q000737:chemistry*; D045483:Rivers; D012475:Salmonella / Q000187:drug effects; D014874:Water Pollutants, Chemical / Q000032:analysis / Q000633:toxicity
chemical_list: D008567:Membranes, Artificial; D011084:Polycyclic Aromatic Hydrocarbons; D014874:Water Pollutants, Chemical; D020959:Polyethylene
keywords: Ames assay; Genotoxicity; PAHs; Passive samplers; River water; Umu test