title: Probing Chromatin Modifications in Response to ERK Signaling.
abstract: Chromatin immunoprecipitation (ChIP) is a technique used to determine the association of proteins or histone modifications with chromatin regions in living cells or tissues, and is used extensively in the chromatin biology field to study transcriptional and epigenetic mechanisms. Increasing evidence points to an epigenetic coordination of signaling cascades, such as ERK, that regulate key processes in development and disease, revealing novel principles of gene regulation. Here we describe a detailed protocol for performing chromatin immunoprecipitation followed by qPCR (ChIP-qPCR) for probing histone modifications regulated by ERK signaling in mouse ESCs.
authors.affiliation: Department of Biochemistry and Molecular Pharmacology, Howard Hughes Medical Institute, New York University School of Medicine, New York, NY, 10016, USA.
authors.forename: Ozgur
authors.initials: O
authors.lastname: Oksuz
authors.affiliation: Institute of Molecular and Cell Biology (IMCB), A*STAR (Agency for Science, Technology, and Research), 61 Biopolis Drive, Proteos, Singapore, 138673, Singapore. wwtee@imcb.a-star.edu.sg.|Department of Physiology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore, 117597, Singapore. wwtee@imcb.a-star.edu.sg.
authors.forename: Wee-Wei
authors.initials: WW
authors.lastname: Tee
mesh_terms: D000818:Animals; D002460:Cell Line; D002843:Chromatin / Q000235:genetics* / Q000378:metabolism*; D047369:Chromatin Immunoprecipitation; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism*; D059014:High-Throughput Nucleotide Sequencing; D042421:Histone Code; D006657:Histones / Q000378:metabolism; D051379:Mice; D015398:Signal Transduction
chemical_list: D002843:Chromatin; D006657:Histones; D048049:Extracellular Signal-Regulated MAP Kinases
keywords: ChIP-Western blot; ChIP-qPCR; Chromatin immunoprecipitation; ERK signaling; Histone modifications; Polycomb; Spike-in normalization