title: Immune-Related Gene Expression Patterns in GPV- or H9N2-Infected Goose Spleens.
abstract: Goose parvovirus (GPV) and avian influenza virus subtype H9N2 are single-stranded DNA (ssDNA) and single-stranded RNA (ssRNA) viruses, respectively, both of which can spread in goslings and cause a significant economic loss. To explore the comprehensive transcriptome of GPV- or H9N2-infected goose spleens and to understand the immune responses induced by a DNA virus (GPV) or a RNA virus (H9N2), RNA-seq was performed on the spleens of goslings at the fifth day post infection. In the present study, 2604 and 2409 differentially expressed unigenes were identified in the GPV- and H9N2-infected groups, respectively. Through KEGG pathway enrichment analyses, the up-regulated transcripts in the two virus-infected groups were mainly involved in immune-related pathways. In addition, the two virus-infected groups displayed similar expression patterns in the immune response pathways, including pattern-recognition receptor signaling pathways, the antigen processing and presentation pathway, the NF-κB signaling pathway and the JAK-STAT signaling pathway, as well as cytokines. Furthermore, most of the immune-related genes, particularly TLR7, TRAF3, Mx, TRIM25, CD4, and CD8α, increased in response to GPV and H9N2 infection. However, the depression of NF-κB signaling may be a mechanism by which the viruses evade the host immune system or a strategy to achieve immune homeostasis.
authors.affiliation: Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China. sophia_cs@163.com.|Avian Disease Research Center, College of Veterinary Medicine of Sichuan Agricultural University, Chengdu 611130, China. sophia_cs@163.com.|Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Chengdu 611130, China. sophia_cs@163.com.
authors.forename: Shun
authors.initials: S
authors.lastname: Chen
authors.affiliation: Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China. anqiwang77@163.com.
authors.forename: Anqi
authors.initials: A
authors.lastname: Wang
authors.affiliation: Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China. 13408051770@163.com.
authors.forename: Lipei
authors.initials: L
authors.lastname: Sun
authors.affiliation: Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Chengdu 611130, China. liufei@sicau.edu.cn.
authors.forename: Fei
authors.initials: F
authors.lastname: Liu
authors.affiliation: Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China. mshwang@163.com.|Avian Disease Research Center, College of Veterinary Medicine of Sichuan Agricultural University, Chengdu 611130, China. mshwang@163.com.|Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Chengdu 611130, China. mshwang@163.com.
authors.forename: Mingshu
authors.initials: M
authors.lastname: Wang
authors.affiliation: Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China. cqrc_jry@163.com.|Avian Disease Research Center, College of Veterinary Medicine of Sichuan Agricultural University, Chengdu 611130, China. cqrc_jry@163.com.|Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Chengdu 611130, China. cqrc_jry@163.com.
authors.forename: Renyong
authors.initials: R
authors.lastname: Jia
authors.affiliation: Avian Disease Research Center, College of Veterinary Medicine of Sichuan Agricultural University, Chengdu 611130, China. zdk24@163.com.|Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Chengdu 611130, China. zdk24@163.com.
authors.forename: Dekang
authors.initials: D
authors.lastname: Zhu
authors.affiliation: Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China. liumafengra@163.com.|Avian Disease Research Center, College of Veterinary Medicine of Sichuan Agricultural University, Chengdu 611130, China. liumafengra@163.com.|Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Chengdu 611130, China. liumafengra@163.com.
authors.forename: Mafeng
authors.initials: M
authors.lastname: Liu
authors.affiliation: Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China. yangqiao721521@sina.com.|Avian Disease Research Center, College of Veterinary Medicine of Sichuan Agricultural University, Chengdu 611130, China. yangqiao721521@sina.com.|Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Chengdu 611130, China. yangqiao721521@sina.com.
authors.forename: Qiao
authors.initials: Q
authors.lastname: Yang
authors.affiliation: Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China. yingzi_no1@126.com.|Avian Disease Research Center, College of Veterinary Medicine of Sichuan Agricultural University, Chengdu 611130, China. yingzi_no1@126.com.|Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Chengdu 611130, China. yingzi_no1@126.com.
authors.forename: Ying
authors.initials: Y
authors.lastname: Wu
authors.affiliation: Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China. sunkunfeng1981@163.com.|Avian Disease Research Center, College of Veterinary Medicine of Sichuan Agricultural University, Chengdu 611130, China. sunkunfeng1981@163.com.|Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Chengdu 611130, China. sunkunfeng1981@163.com.
authors.forename: Kunfeng
authors.initials: K
authors.lastname: Sun
authors.affiliation: Avian Disease Research Center, College of Veterinary Medicine of Sichuan Agricultural University, Chengdu 611130, China. sophia_cs@yeah.net.|Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Chengdu 611130, China. sophia_cs@yeah.net.
authors.forename: Xiaoyue
authors.initials: X
authors.lastname: Chen
authors.affiliation: Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China. chenganchun@vip.163.com.|Avian Disease Research Center, College of Veterinary Medicine of Sichuan Agricultural University, Chengdu 611130, China. chenganchun@vip.163.com.|Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Chengdu 611130, China. chenganchun@vip.163.com.
authors.forename: Anchun
authors.initials: A
authors.lastname: Cheng
mesh_terms: D000818:Animals; D016207:Cytokines / Q000096:biosynthesis; D005777:Geese / Q000378:metabolism* / Q000821:virology; D005786:Gene Expression Regulation; D007113:Immunity, Innate / Q000235:genetics; D053127:Influenza A Virus, H9N2 Subtype / Q000235:genetics / Q000472:pathogenicity; D005585:Influenza in Birds / Q000235:genetics* / Q000821:virology; D016328:NF-kappa B / Q000096:biosynthesis; D017992:Parvovirus / Q000235:genetics / Q000472:pathogenicity; D015398:Signal Transduction / Q000235:genetics; D013154:Spleen / Q000378:metabolism* / Q000821:virology; D059467:Transcriptome / Q000235:genetics*
chemical_list: D016207:Cytokines; D016328:NF-kappa B
keywords: GPV; H9N2; identical expression profile; systemic transcriptome