title: The CSRP2BP histone acetyltransferase drives smooth muscle gene expression.
abstract: The expression of nearly all smooth muscle genes are controlled by serum response factor binding sites in their promoter regions. However, SRF alone is not sufficient for regulating smooth muscle cell development. It associates with other cardiovascular specific cofactors to regulate smooth muscle gene expression. Previously, we showed that the transcription co-factor CRP2 was a regulator of smooth muscle gene expression. Here, we report that CSRP2BP, a coactivator for CRP2, is a histone acetyltransferase and a driver of smooth muscle gene expression. CSRP2BP directly interacted with SRF, CRP2 and myocardin. CSRP2BP synergistically activated smooth muscle gene promoters in an SRF-dependent manner. A combination of SRF, GATA6 and CRP2 required CSRP2BP for robust smooth muscle gene promoter activity. Knock-down of Csrp2bp in smooth muscle cells resulted in reduced smooth muscle gene expression. We conclude that the CSRP2BP histone acetyltransferase is a coactivator for CRP2 that works synergistically with SRF and myocardin to regulate smooth muscle gene expression.
authors.affiliation: Department of Obstetrics and Gynecology, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong 510515, China.|Hainan Provincial Key Laboratory for Human Reproductive Medicine and Genetic Research, The Key Laboratory of Tropical Diseases and Translational Medicine of The Ministry of Education, Affiliated Hospital of Hainan Medical University, Hainan Medical University, Haikou, Hainan 570102, China.|The Institute of Biosciences and Technology, Texas A&M University System Health Science Center, Houston, TX 77030, USA.
authors.forename: Yanlin
authors.initials: Y
authors.lastname: Ma
authors.affiliation: Hainan Provincial Key Laboratory for Human Reproductive Medicine and Genetic Research, The Key Laboratory of Tropical Diseases and Translational Medicine of The Ministry of Education, Affiliated Hospital of Hainan Medical University, Hainan Medical University, Haikou, Hainan 570102, China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Li
authors.affiliation: Graduate Program in Cardiovascular Sciences, Baylor College of Medicine, Houston, TX 77030, USA.
authors.forename: Ankang
authors.initials: A
authors.lastname: Li
authors.affiliation: Hainan Provincial Key Laboratory for Human Reproductive Medicine and Genetic Research, The Key Laboratory of Tropical Diseases and Translational Medicine of The Ministry of Education, Affiliated Hospital of Hainan Medical University, Hainan Medical University, Haikou, Hainan 570102, China.
authors.forename: Yunjian
authors.initials: Y
authors.lastname: Wei
authors.affiliation: Hainan Provincial Key Laboratory for Human Reproductive Medicine and Genetic Research, The Key Laboratory of Tropical Diseases and Translational Medicine of The Ministry of Education, Affiliated Hospital of Hainan Medical University, Hainan Medical University, Haikou, Hainan 570102, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Long
authors.affiliation: Hainan Provincial Key Laboratory for Human Reproductive Medicine and Genetic Research, The Key Laboratory of Tropical Diseases and Translational Medicine of The Ministry of Education, Affiliated Hospital of Hainan Medical University, Hainan Medical University, Haikou, Hainan 570102, China.
authors.forename: Xinxing
authors.initials: X
authors.lastname: Jiang
authors.affiliation: Department of Obstetrics and Gynecology, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong 510515, China.
authors.forename: Fei
authors.initials: F
authors.lastname: Sun
authors.affiliation: Institute of Molecular Pathobiochemistry, Experimental Gene Therapy and Clinical Chemistry, University Hospital Aachen, Pauwelsstr. 30, D-52074 Aachen, Germany.
authors.forename: Ralf
authors.initials: R
authors.lastname: Weiskirchen
authors.affiliation: Hainan Provincial Key Laboratory for Human Reproductive Medicine and Genetic Research, The Key Laboratory of Tropical Diseases and Translational Medicine of The Ministry of Education, Affiliated Hospital of Hainan Medical University, Hainan Medical University, Haikou, Hainan 570102, China.
authors.forename: Bangyong
authors.initials: B
authors.lastname: Wu
authors.affiliation: Hainan Provincial Key Laboratory for Human Reproductive Medicine and Genetic Research, The Key Laboratory of Tropical Diseases and Translational Medicine of The Ministry of Education, Affiliated Hospital of Hainan Medical University, Hainan Medical University, Haikou, Hainan 570102, China.
authors.forename: Chao
authors.initials: C
authors.lastname: Liang
authors.affiliation: Institute of Biochemistry, Christian-Albrechts-Universität zu Kiel, Olshausenstr. 40, 24098 Kiel, Germany.
authors.forename: Joachim
authors.initials: J
authors.lastname: Grötzinger
authors.affiliation: Department of Obstetrics and Gynecology, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong 510515, China.
authors.forename: Yanxing
authors.initials: Y
authors.lastname: Wei
authors.affiliation: Department of Biology and Biochemistry, University of Houston, Houston, TX 77204, USA.
authors.forename: Wei
authors.initials: W
authors.lastname: Yu
authors.affiliation: Stem Cell and Regeneration Program, The Burnham Institute for Medical Research, 10901 North Torrey Pines Road, La Jolla, CA 92037, USA.
authors.forename: Mark
authors.initials: M
authors.lastname: Mercola
authors.affiliation: Hainan Provincial Key Laboratory for Human Reproductive Medicine and Genetic Research, The Key Laboratory of Tropical Diseases and Translational Medicine of The Ministry of Education, Affiliated Hospital of Hainan Medical University, Hainan Medical University, Haikou, Hainan 570102, China.
authors.forename: Yuanhua
authors.initials: Y
authors.lastname: Huang
authors.affiliation: Texas Heart Institute, Houston, TX 77030, USA.
authors.forename: Jun
authors.initials: J
authors.lastname: Wang
authors.affiliation: Hainan Provincial Key Laboratory for Human Reproductive Medicine and Genetic Research, The Key Laboratory of Tropical Diseases and Translational Medicine of The Ministry of Education, Affiliated Hospital of Hainan Medical University, Hainan Medical University, Haikou, Hainan 570102, China.
authors.forename: Yanhong
authors.initials: Y
authors.lastname: Yu
authors.affiliation: Stem Cell and Regeneration Program, The Burnham Institute for Medical Research, 10901 North Torrey Pines Road, La Jolla, CA 92037, USA.|Texas Heart Institute, Houston, TX 77030, USA.
authors.forename: Robert J
authors.initials: RJ
authors.lastname: Schwartz
mesh_terms: D000107:Acetylation; D000818:Animals; D002460:Cell Line; D002467:Cell Nucleus / Q000201:enzymology; D002478:Cells, Cultured; D002843:Chromatin / Q000201:enzymology; D015870:Gene Expression; D005786:Gene Expression Regulation; D051548:Histone Acetyltransferases / Q000378:metabolism*; D006657:Histones / Q000378:metabolism; D006801:Humans; D051379:Mice; D032389:Myocytes, Smooth Muscle / Q000201:enzymology / Q000378:metabolism*; D009687:Nuclear Proteins / Q000378:metabolism; D011401:Promoter Regions, Genetic; D051381:Rats; D015534:Trans-Activators / Q000378:metabolism; D014157:Transcription Factors / Q000378:metabolism; D000099251:Myocardin
chemical_list: D002843:Chromatin; D051548:Histone Acetyltransferases; D006657:Histones; D009687:Nuclear Proteins; D015534:Trans-Activators; D014157:Transcription Factors; D000099251:Myocardin; C000621372:Csrp2bp protein, mouse