title: Heparin-Like Chitosan Hydrogels with Tunable Swelling Behavior, Prolonged Clotting Times, and Prevented Contact Activation and Complement Activation.
abstract: The aim of this study was to create heparin-like chitosan hydrogels (HLCHs) for blood purification. Herein, we prepared two heparin-like chitosans (HLCSs) with various carboxymethyl and sulfate groups, followed by a cross-linking reaction with glutaraldehyde. The synthetic chitosan derivatives were characterized by X-ray photoelectron spectroscopy, gel permeation chromatography, FTIR and NMR. The average sulfonation degrees of two HLCSs were 0.69 and 0.94 per sugar unit, respectively. The swelling ratio of the HLCH could reach up to 4800%, and the HLCHs remained a well-defined shape and stable below 170 °C. Moreover, the activated partial thromboplastin time and thrombin time results indicated that both of the HLCSs and their hydrogels exhibited excellent thrombus inhibition property. Furthermore, the contact activation and complement activation results also proved that the hydrogels possessed good blood compatibility and had the potential to be used as blood-contacting materials.
authors.affiliation: College of Polymer Science and Engineering, State Key Laboratory of Polymer Materials Engineering, Sichuan University , Chengdu 610065, China.
authors.forename: Xuelian
authors.initials: X
authors.lastname: Huang
authors.affiliation: College of Polymer Science and Engineering, State Key Laboratory of Polymer Materials Engineering, Sichuan University , Chengdu 610065, China.
authors.forename: Rui
authors.initials: R
authors.lastname: Wang
authors.affiliation: College of Polymer Science and Engineering, State Key Laboratory of Polymer Materials Engineering, Sichuan University , Chengdu 610065, China.
authors.forename: Ting
authors.initials: T
authors.lastname: Lu
authors.affiliation: College of Polymer Science and Engineering, State Key Laboratory of Polymer Materials Engineering, Sichuan University , Chengdu 610065, China.
authors.forename: Dongxu
authors.initials: D
authors.lastname: Zhou
authors.affiliation: College of Polymer Science and Engineering, State Key Laboratory of Polymer Materials Engineering, Sichuan University , Chengdu 610065, China.
authors.forename: Weifeng
authors.initials: W
authors.lastname: Zhao
authors.affiliation: College of Polymer Science and Engineering, State Key Laboratory of Polymer Materials Engineering, Sichuan University , Chengdu 610065, China.
authors.forename: Shudong
authors.initials: S
authors.lastname: Sun
authors.affiliation: College of Polymer Science and Engineering, State Key Laboratory of Polymer Materials Engineering, Sichuan University , Chengdu 610065, China.
authors.forename: Changsheng
authors.initials: C
authors.lastname: Zhao
mesh_terms: D001672:Biocompatible Materials / Q000737:chemistry*; D001777:Blood Coagulation; D048271:Chitosan / Q000737:chemistry*; D003167:Complement Activation; D006493:Heparin / Q000737:chemistry*; D006801:Humans; D020100:Hydrogels / Q000737:chemistry*; D009994:Osmolar Concentration; D014914:Whole Blood Coagulation Time
chemical_list: D001672:Biocompatible Materials; D020100:Hydrogels; D006493:Heparin; D048271:Chitosan