title: Discovering Functional ERK Substrates Regulating Caenorhabditis elegans Germline Development.
abstract: The Rat Sarcoma (RAS) GTPAse-mediated extracellular signal-regulated kinase (ERK) pathway regulates multiple biological processes across metazoans. In particular during Caenorhabditis elegans oogenesis, ERK signaling has been shown to regulate over seven distinct biological processes in a temporal and sequential manner. To fully elucidate how ERK signaling cascade orchestrates these different biological processes in vivo, identification of the direct functional substrates of the pathway is critical. This chapter describes the methods that were used to identify ERK substrates in a global manner and study their functions in the germline. These approaches can also be generally applied to study ERK-dependent biological processes in other systems.
authors.affiliation: The University of Texas Graduate School of Biomedical Sciences, Houston, TX, USA.
authors.forename: Jessica Jie
authors.initials: JJ
authors.lastname: Chen
authors.affiliation: The University of Texas Graduate School of Biomedical Sciences, Houston, TX, USA. sarur@mdanderson.org.|Department of Genetics, The University of Texas MD Anderson Cancer Center, 1515 Holcombe Blvd., Houston, TX, 77030, USA. sarur@mdanderson.org.
authors.forename: Swathi
authors.initials: S
authors.lastname: Arur
mesh_terms: D000818:Animals; D017173:Caenorhabditis elegans / Q000196:embryology / Q000235:genetics / Q000378:metabolism*; D029742:Caenorhabditis elegans Proteins / Q000235:genetics / Q000378:metabolism*; D002352:Carrier Proteins; D019295:Computational Biology / Q000379:methods*; D030541:Databases, Genetic; D004789:Enzyme Activation; D048049:Extracellular Signal-Regulated MAP Kinases / Q000235:genetics / Q000378:metabolism*; D005786:Gene Expression Regulation; D005854:Germ Cells / Q000378:metabolism*; D007150:Immunohistochemistry; D009866:Oogenesis / Q000235:genetics; D011485:Protein Binding; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics; D012984:Software; D013379:Substrate Specificity; D064878:Web Browser
chemical_list: D029742:Caenorhabditis elegans Proteins; D002352:Carrier Proteins; D034741:RNA, Small Interfering; D048049:Extracellular Signal-Regulated MAP Kinases
keywords: C. elegans oogenesis; ERK substrates; Functional genomics