title: Next-generation sequence detectsARAP3as a novel oncogene in papillary thyroid carcinoma.
abstract: OBJECTIVE
  Thyroid cancer is the most frequent malignancies of the endocrine system, and it has became the fastest growing type of cancer worldwide. Much still remains unknown about the molecular mechanisms of thyroid cancer. Studies have found that some certain relationship betweenARAP3and human cancer. However, the role ofARAP3in thyroid cancer has not been well explained. This study aimed to investigate the role ofARAP3gene in papillary thyroid carcinoma.
  
  
  METHODS
  Whole exon sequence and whole genome sequence of primary papillary thyroid carcinoma (PTC) samples and matched adjacent normal thyroid tissue samples were performed and then bioinformatics analysis was carried out. PTC cell lines (TPC1, BCPAP, and KTC-1) with transfection of small interfering RNA were used to investigate the functions ofARAP3gene, including cell proliferation assay, colony formation assay, migration assay, and invasion assay.
  
  
  RESULTS
  Using next-generation sequence and bioinformatics analysis, we foundARAP3genes may play an important role in thyroid cancer. Downregulation ofARAP3significantly suppressed PTC cell lines (TPC1, BCPAP, and KTC-1), cell proliferation, colony formation, migration, and invasion.
  
  
  CONCLUSIONS
  This study indicated thatARAP3genes have important biological implications and may act as a potentially drugable target in PTC.
authors.affiliation: Department of Oncology, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang Province, China.
authors.forename: Qing-Xuan
authors.initials: QX
authors.lastname: Wang
authors.affiliation: Department of Oncology, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang Province, China.
authors.forename: En-Dong
authors.initials: ED
authors.lastname: Chen
authors.affiliation: Department of Oncology, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang Province, China.
authors.forename: Ye-Feng
authors.initials: YF
authors.lastname: Cai
authors.affiliation: Department of Oncology, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang Province, China.
authors.forename: Yi-Li
authors.initials: YL
authors.lastname: Zhou
authors.affiliation: Department of Oncology, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang Province, China.
authors.forename: Zhou-Ci
authors.initials: ZC
authors.lastname: Zheng
authors.affiliation: Department of Oncology, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang Province, China.
authors.forename: Ying-Hao
authors.initials: YH
authors.lastname: Wang
authors.affiliation: Department of Oncology, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang Province, China.
authors.forename: Yi-Xiang
authors.initials: YX
authors.lastname: Jin
authors.affiliation: Department of Oncology, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang Province, China.
authors.forename: Wen-Xu
authors.initials: WX
authors.lastname: Jin
authors.affiliation: Department of Oncology, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang Province, China.
authors.forename: Xiao-Hua
authors.initials: XH
authors.lastname: Zhang
authors.affiliation: Department of Oncology, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang Province, China.
authors.forename: Ou-Chen
authors.initials: OC
authors.lastname: Wang
keywords: ARAP3; next-generation sequence; oncogene; papillary thyroid carcinoma