title: Bioprospecting of Turbinaria Macroalgae as a Potential Source of Health Protective Compounds.
abstract: The present study aims to focus on the bioprospecting of marine macroalgae of Turbinaria species, plenteous biomass of the world ocean. Three types of solvents, i.e., H2O, MeOH/H2O (80:20, v/v) and hexane/i-PrOH (50:50, v/v), were used for extraction. Both the biological activity and the pattern of present chemicals were characterized. For the cell proliferation assay, the human embryonic kidney 293 cells, cervix/breast/pancreatic adenocarcinoma, and osteosarcoma cells were used. For the antioxidant activity determination, both intracellular assay with human embryonic kidney and cervix adenocarcinoma cells, as well as the biochemical DPPH test, were employed. To complete the information about macroalgae composition, organic compounds were characterized by the liquid chromatography coupled with high resolution tandem mass spectrometry. Attention was concentrated mainly on the lipidomic profile characterization. In spite the fact that any significant antiproliferative effect was not observed for cancer cells, both the Turbinaria species were shown to be good protectors against the oxidative stress of the non-cancer cells. Most of the antioxidants were determined in the hexane/i-PrOH extract. As regards the lipids identified, most of them belonged to the triacylglycerols followed by sphingomyelins, diacylglycerols, and polar (lyso)phospholipids. Additionally to fatty acids with 14, 16 and 18 carbons, also those with odd carbon numbers were frequently present.
authors.affiliation: Department of Food Analysis and Nutrition, University of Chemistry and Technology, Prague, Technicka 3, 166 28, Prague, Czech Republic.
authors.forename: Milena
authors.initials: M
authors.lastname: Stranska-Zachariasova
authors.affiliation: School of Life Sciences and Technology, Institut Teknologi Bandung, Jl. Tamansari 64, 40116, Bandung, Indonesia.
authors.forename: Isma
authors.initials: I
authors.lastname: Kurniatanty
authors.affiliation: Department of Biochemistry and Microbiology, University of Chemistry and Technology, Prague, Technicka 3, 166 28, Prague, Czech Republic.
authors.forename: Helena
authors.initials: H
authors.lastname: Gbelcova
authors.affiliation: Department of Food Analysis and Nutrition, University of Chemistry and Technology, Prague, Technicka 3, 166 28, Prague, Czech Republic.
authors.forename: Monika
authors.initials: M
authors.lastname: Jiru
authors.affiliation: Department of Food Analysis and Nutrition, University of Chemistry and Technology, Prague, Technicka 3, 166 28, Prague, Czech Republic.
authors.forename: Josep
authors.initials: J
authors.lastname: Rubert
authors.affiliation: Department of Mechanical Engineering, Engineering Faculty, Udayana University, Jimbaran, Bali, 80361, Indonesia.
authors.forename: Tjokorda Gde Tirta
authors.initials: TG
authors.lastname: Nindhia
authors.affiliation: Department of Biochemistry and Microbiology, University of Chemistry and Technology, Prague, Technicka 3, 166 28, Prague, Czech Republic.
authors.forename: Cosimo Walter
authors.initials: CW
authors.lastname: D'Acunto
authors.affiliation: School of Life Sciences and Technology, Institut Teknologi Bandung, Jl. Tamansari 64, 40116, Bandung, Indonesia.
authors.forename: Sony Heru
authors.initials: SH
authors.lastname: Sumarsono
authors.affiliation: School of Life Sciences and Technology, Institut Teknologi Bandung, Jl. Tamansari 64, 40116, Bandung, Indonesia.
authors.forename: Marselina Irasonia
authors.initials: MI
authors.lastname: Tan
authors.affiliation: Department of Food Analysis and Nutrition, University of Chemistry and Technology, Prague, Technicka 3, 166 28, Prague, Czech Republic.
authors.forename: Jana
authors.initials: J
authors.lastname: Hajslova
authors.affiliation: Department of Biochemistry and Microbiology, University of Chemistry and Technology, Prague, Technicka 3, 166 28, Prague, Czech Republic.
authors.forename: Tomas
authors.initials: T
authors.lastname: Ruml
mesh_terms: D000975:Antioxidants / Q000494:pharmacology*; D001688:Biological Products / Q000737:chemistry*; D065132:Bioprospecting / Q000379:methods*; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D008055:Lipids / Q000032:analysis / Q000737:chemistry; D055442:Metabolome; D055432:Metabolomics; D058086:Microalgae / Q000737:chemistry* / Q000378:metabolism; D020011:Protective Agents / Q000494:pharmacology*; D053719:Tandem Mass Spectrometry
chemical_list: D000975:Antioxidants; D001688:Biological Products; D008055:Lipids; D020011:Protective Agents
keywords: Algae; Antioxidant activities; Antiproliferation assay; Bioprospecting; Liquid chromatography; Mass spectrometry; Turbinaria