title: [Protective effect of astragaloside IV against acute liver failure in experimental mice].
abstract: Objective:To investigate the clinical effect of astragaloside IV in the early treatment of mice with acute liver failure and possible mechanisms.Methods:A mouse model of acute liver failure induced by D-galactosamine/lipopolysaccharide (D-GalN/LPS) was established, and the mice were given astragaloside IV at different doses. The survival rate of mice, alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels, liver histopathological changes, apoptosis of hepatocytes, and the expression of superoxide dismutase (SOD) and malondialdehyde (MDA) were measured in each group. The least significant difference test was used for data with homogeneity of variances, the Dunnett's T3 test was used for data with heterogeneity of variance, and the Kaplan-Meier method was used for survival analysis.Results:Compared with the model group, the high-dose astragaloside IV group had a significant increase in the 48-hour survival rate [60% (9/15) vs 13.3% (2/15),P< 0.05], significant reductions in the serum ALT and AST levels (P< 0.01), and significant reductions in liver histopathological indices and the degree of apoptosis of hepatocytes (P< 0.01), as well as a significant reduction in the content of MDA in liver homogenate (P< 0.01) and a significant increase in the activity of SOD (P< 0.05).Conclusion:High-dose astragaloside IV has a significant protective effect against D-GalN/LPS-induced acute liver injury in mice, and its mechanisms may be associated with its effects against cell apoptosis and oxidative damage.
authors.affiliation: Pediatric Department of Zhuji People's Hospital, Zhuji City, Zhejiang Province 311800, China.
authors.forename: L
authors.initials: L
authors.lastname: Liu
authors.affiliation: Department of Hepatology, Children's Hospital of Hunan Province , Changsha 410007, China.
authors.forename: S J
authors.initials: SJ
authors.lastname: Li
authors.affiliation: Intensive Care Unit of Zhuji People's Hospital, Zhuji City, Zhejiang Province 311800, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Zhou
mesh_terms: D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D017209:Apoptosis; D001219:Aspartate Aminotransferases / Q000097:blood; D004195:Disease Models, Animal; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000494:pharmacology*; D005688:Galactosamine / Q000009:adverse effects / Q000494:pharmacology; D022781:Hepatocytes; D008070:Lipopolysaccharides / Q000009:adverse effects / Q000494:pharmacology; D008099:Liver / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D017114:Liver Failure, Acute / Q000139:chemically induced / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D008315:Malondialdehyde; D051379:Mice; D012503:Saponins / Q000008:administration & dosage / Q000494:pharmacology*; D013482:Superoxide Dismutase / Q000097:blood / Q000378:metabolism; D014315:Triterpenes / Q000008:administration & dosage / Q000494:pharmacology*
chemical_list: D004365:Drugs, Chinese Herbal; D008070:Lipopolysaccharides; D012503:Saponins; D014315:Triterpenes; C052064:astragaloside A; D008315:Malondialdehyde; D005688:Galactosamine; D013482:Superoxide Dismutase; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase