title: Presence of T-275A and C-2152T Polymorphisms of the Promoter Region of Uridine Diphosphate-Glucuronosyltransferase 1A9 Increases Mortality From Digestive Tumors: Results After 10 Years of Follow-up in a Renal Transplant Population.
abstract: BACKGROUND
  The aim of this study was to determine the distribution of uridine diphosphate-glucuronosyltransferase 1A9 (UGT1A9) promoter region T-275A and C-2152T single-nucleotide polymorphisms (SNPs) in stable transplant patients and to investigate the impact of these SNPs on the evolution of this population after 10 years of follow-up.
  
  
  METHODS
  White renal transplant recipients (n = 873) were studied. The median time of follow-up was 91.8 months (P25-75 46-146). Amplification with specific "primers" to delimit the study area was performed for each polymorphism. Amplification was performed with the use of real-time polymerase chain reaction.
  
  
  RESULTS
  T-275A promoter mutation was detected in 13% of patients and C-2152T in 12% of patients. Survival analysis was performed on 873 renal transplants, carried out between 2004 and 2013. We found a higher frequency of death from cancer among polymorphism carriers (P = .001).
  
  
  CONCLUSIONS
  It appears that carriers of T-275A and C-2152T SNPs of the UGT1A9 gene promoter region show a greater incidence of death from cancer, with a significantly higher cumulative incidence of death from gastrointestinal tumors.
authors.affiliation: Nephrology Department, Hospital Clinico San Carlos, Madrid, Spain.
authors.forename: N
authors.initials: N
authors.lastname: Calvo
authors.affiliation: Nephrology Department, Hospital Clinico San Carlos, Madrid, Spain.
authors.forename: A
authors.initials: A
authors.lastname: Shabaka
authors.affiliation: Nephrology Department, Hospital Clinico San Carlos, Madrid, Spain.
authors.forename: B
authors.initials: B
authors.lastname: Rodriguez Cubillo
authors.affiliation: Nephrology Department, Hospital Clinico San Carlos, Madrid, Spain.
authors.forename: V
authors.initials: V
authors.lastname: López de la Manzanara
authors.affiliation: Nephrology Department, Hospital Clinico San Carlos, Madrid, Spain.
authors.forename: I
authors.initials: I
authors.lastname: Pérez-Flores
authors.affiliation: Nephrology Department, Hospital Clinico San Carlos, Madrid, Spain.
authors.forename: M A
authors.initials: MA
authors.lastname: Moreno de la Higuera
authors.affiliation: Nephrology Department, Hospital Clinico San Carlos, Madrid, Spain.
authors.forename: J
authors.initials: J
authors.lastname: Bautista
authors.affiliation: Nephrology Department, Hospital Clinico San Carlos, Madrid, Spain.
authors.forename: A I
authors.initials: AI
authors.lastname: Sánchez-Fructuoso
mesh_terms: D000328:Adult; D017931:DNA Primers; D004067:Digestive System Neoplasms / Q000235:genetics* / Q000401:mortality; D005260:Female; D005500:Follow-Up Studies; D014453:Glucuronosyltransferase / Q000235:genetics*; D006801:Humans; D016030:Kidney Transplantation; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D011183:Postoperative Complications / Q000235:genetics* / Q000401:mortality; D011401:Promoter Regions, Genetic / Q000235:genetics*; D060888:Real-Time Polymerase Chain Reaction; D000090264:UDP-Glucuronosyltransferase 1A9; D044465:White People / Q000235:genetics
chemical_list: D017931:DNA Primers; C000715591:UGT1A9 protein, human; D014453:Glucuronosyltransferase; D000090264:UDP-Glucuronosyltransferase 1A9