title: Complex Genetics of Behavior: BXDs in the Automated Home-Cage.
abstract: This chapter describes a use case for the genetic dissection and automated analysis of complex behavioral traits using the genetically diverse panel of BXD mouse recombinant inbred strains. Strains of the BXD resource differ widely in terms of gene and protein expression in the brain, as well as in their behavioral repertoire. A large mouse resource opens the possibility for gene finding studies underlying distinct behavioral phenotypes, however, such a resource poses a challenge in behavioral phenotyping. To address the specifics of large-scale screening we describe how to investigate: (1) how to assess mouse behavior systematically in addressing a large genetic cohort, (2) how to dissect automation-derived longitudinal mouse behavior into quantitative parameters, and (3) how to map these quantitative traits to the genome, deriving loci underlying aspects of behavior.
authors.affiliation: Sylics (Synaptologics BV), 71033, 1008 BA, Amsterdam, The Netherlands. maarten.loos@sylics.com.|Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research (CNCR), Neuroscience Campus Amsterdam, VU University Amsterdam, Amsterdam, The Netherlands. maarten.loos@sylics.com.|NeuroBsik Mouse Phenomics Consortium:, A list of additional members of the Neuro-BSIK Mouse Phenomics Consortium is provided in the Acknowledgments., Wageningen, Netherlands. maarten.loos@sylics.com.
authors.forename: Maarten
authors.initials: M
authors.lastname: Loos
authors.affiliation: Department of Functional Genomics, Center for Neurogenomics and Cognitive Research (CNCR), Neuroscience Campus Amsterdam, VU University Amsterdam, Amsterdam, The Netherlands.|Department of Clinical Genetics, VU Medical Center, Amsterdam, The Netherlands.|NeuroBsik Mouse Phenomics Consortium:, A list of additional members of the Neuro-BSIK Mouse Phenomics Consortium is provided in the Acknowledgments., Wageningen, Netherlands.
authors.forename: Matthijs
authors.initials: M
authors.lastname: Verhage
authors.affiliation: Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research (CNCR), Neuroscience Campus Amsterdam, VU University Amsterdam, Amsterdam, The Netherlands.|NeuroBsik Mouse Phenomics Consortium:, A list of additional members of the Neuro-BSIK Mouse Phenomics Consortium is provided in the Acknowledgments., Wageningen, Netherlands.
authors.forename: Sabine
authors.initials: S
authors.lastname: Spijker
authors.affiliation: Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research (CNCR), Neuroscience Campus Amsterdam, VU University Amsterdam, Amsterdam, The Netherlands.|NeuroBsik Mouse Phenomics Consortium:, A list of additional members of the Neuro-BSIK Mouse Phenomics Consortium is provided in the Acknowledgments., Wageningen, Netherlands.
authors.forename: August B
authors.initials: AB
authors.lastname: Smit
mesh_terms: D000818:Animals; D001522:Behavior, Animal; D003433:Crosses, Genetic; D056726:Genetic Association Studies / Q000379:methods; D051379:Mice; D008815:Mice, Inbred Strains; D040641:Quantitative Trait Loci; D019655:Quantitative Trait, Heritable; D012984:Software; D064878:Web Browser
keywords: Automated home-cage; Genetic effect size; High-throughput; Inbred mouse strains; Mouse phenomics; PhenoTyper; Reference database; Sheltering; Spontaneous behavior; Systematic behavioral profile