title: Structural dynamics and DNA interaction of human TFIID.
abstract: TFIID is a large protein complex required for the recognition and binding of eukaryotic gene core promoter sequences and for the recruitment of the rest of the general transcription factors involved in initiation of eukaryotic protein gene transcription. Cryo-electron microscopy studies have demonstrated the conformational complexity of human TFIID, where one-third of the mass of the complex can shift its position by well over 100 Å. This conformational plasticity appears to be linked to the capacity of TFIID to bind DNA, and suggests how it would allow both the recognition of different core promoter elements and the tuning of its binding affinity by regulatory factors.
authors.affiliation: a Molecular and Cell Biology Department and QB3 Institute , UC Berkeley , CA , USA.|b Howard Hughes Medical Institute , UC Berkeley , CA , USA.|c Molecular Biophysics and Integrative Bio-Imaging Division , Lawrence Berkeley National Lab , CA , USA.
authors.forename: Eva
authors.initials: E
authors.lastname: Nogales
authors.affiliation: b Howard Hughes Medical Institute , UC Berkeley , CA , USA.
authors.forename: Jie
authors.initials: J
authors.lastname: Fang
authors.affiliation: d Biophysics Graduate Group , UC Berkeley , CA , USA.
authors.forename: Robert K
authors.initials: RK
authors.lastname: Louder
mesh_terms: D020285:Cryoelectron Microscopy; D004247:DNA / Q000378:metabolism*; D006367:HeLa Cells; D006801:Humans; D008958:Models, Molecular; D011485:Protein Binding; D011487:Protein Conformation; D035362:Transcription Factor TFIID / Q000737:chemistry* / Q000378:metabolism*; D014158:Transcription, Genetic
chemical_list: D035362:Transcription Factor TFIID; D004247:DNA
keywords: conformational heterogeneity; cryo-EM; pre-initiation complex; transcription initiation