title: Evaluation of sulfur isotopic enrichment of urine metabolites for the differentiation of healthy and prostate cancer mice after the administration of34S labelled yeast.
abstract: Sulfur isotopic enrichment of urine metabolites in healthy and prostate cancer mice using34S enriched yeast and High Performance Liquid Chromatography coupled to Multicollector Inductively Coupled Plasma Mass Spectrometry (HPLC-MC-ICP-MS) has been evaluated. A 30 weeks experiment (since the eleventh to the fortieth week of life) was carried out collecting the urine of three healthy mice and three transgenic mice with prostate cancer during 24h after a single oral administration of a34S enriched yeast slurry. The isotopic enrichment of different sulphur metabolites was monitored by coupling a C18 reverse phase HPLC column with a multicollector ICP-MS using a membrane desolvating system. Quantification of sulfur in the chromatographic peaks was carried out by post-column isotope dilution using a33S enriched spike. Differences between the34S enrichment in the urine metabolites of healthy and prostate cancer mice were found from the beginning of the disease. Both populations could be differentiated using a principal component analysis (PCA). Finally, 7 unknown mice were correctly classified in each population using a linear discriminant analysis.
authors.affiliation: Department of Physical and Analytical Chemistry, University of Oviedo, Julian Clavería 8, 33006 Oviedo, Spain.
authors.forename: Oscar
authors.initials: O
authors.lastname: Galilea San Blas
authors.affiliation: Department of Biochemistry and Molecular Biology, University of Oviedo, Spain.
authors.forename: Fernando
authors.initials: F
authors.lastname: Moreno Sanz
authors.affiliation: Department of Biochemistry and Molecular Biology, University of Oviedo, Spain.
authors.forename: Pilar
authors.initials: P
authors.lastname: Herrero Espílez
authors.affiliation: Department of Morphology and Cellular Biology, University of Oviedo, Spain.
authors.forename: Rosa María
authors.initials: RM
authors.lastname: Sainz Menéndez
authors.affiliation: Department of Morphology and Cellular Biology, University of Oviedo, Spain.
authors.forename: Juan Carlos
authors.initials: JC
authors.lastname: Mayo Barallo
authors.affiliation: Department of Physical and Analytical Chemistry, University of Oviedo, Julian Clavería 8, 33006 Oviedo, Spain.
authors.forename: Juan Manuel
authors.initials: JM
authors.lastname: Marchante-Gayón
authors.affiliation: Department of Physical and Analytical Chemistry, University of Oviedo, Julian Clavería 8, 33006 Oviedo, Spain. Electronic address: jiga@uniovi.es.
authors.forename: José Ignacio
authors.initials: JI
authors.lastname: García Alonso
mesh_terms: D000821:Animal Feed / Q000032:analysis*; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D006262:Health; D008297:Male; D013058:Mass Spectrometry; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D025341:Principal Component Analysis; D011471:Prostatic Neoplasms / Q000378:metabolism* / Q000652:urine*; D012441:Saccharomyces cerevisiae / Q000737:chemistry*; D013460:Sulfur Isotopes / Q000378:metabolism* / Q000652:urine*
chemical_list: D013460:Sulfur Isotopes
keywords: (34)S enriched yeast; HPLC-multicollector-ICP-MS; Mice; Prostate cancer; Urine metabolites