title: LiCl regulates mitochondrial biogenesis during megakaryocyte development.
abstract: JAK-STAT, PI3K-AKT and MAPK signaling pathways are involved in platelet production process. Although wnt signaling has been reported in the biogenesis of platelets, but its role in megakaryocyte development is not well studied. We used an inducible canonical wnt signaling system that utilizes LiCl (GSK-3β inhibitor). LiCl could activate wnt signaling pathway along with maturation of megakaryocytes. Mitochondrial staining showed an increase in mitochondrial mass upon induction with LiCl. Also, mitochondrial markers PGC-1α and TFAM were up regulated with increase in mitochondrial DNA content. LiCl leads to increase in the ROS production, suggesting significance of mitochondria in megakaryocyte development.
authors.affiliation: Stem Cells and Haematological Disorders Laboratory, Department of Biochemistry, School of Life Sciences, University of Hyderabad, PO Gachibowli, Hyderabad-500046, Telangana, India.
authors.forename: Ram Babu
authors.initials: RB
authors.lastname: Undi
authors.affiliation: Department of Biotechnology, GITAM Institute of Science, GITAM University, Visakhapatnam-530 045, Andhra Pradesh, India. Electronic address: guttiusha@gmail.com.
authors.forename: Usha
authors.initials: U
authors.lastname: Gutti
authors.affiliation: Stem Cells and Haematological Disorders Laboratory, Department of Biochemistry, School of Life Sciences, University of Hyderabad, PO Gachibowli, Hyderabad-500046, Telangana, India. Electronic address: guttiravi@gmail.com.
authors.forename: Ravi Kumar
authors.initials: RK
authors.lastname: Gutti
mesh_terms: D004305:Dose-Response Relationship, Drug; D006801:Humans; D018021:Lithium Chloride / Q000494:pharmacology*; D008533:Megakaryocytes / Q000166:cytology* / Q000187:drug effects*; D008928:Mitochondria / Q000187:drug effects*; D001678:Organelle Biogenesis; D013329:Structure-Activity Relationship; D014407:Tumor Cells, Cultured
chemical_list: D018021:Lithium Chloride
keywords: LiCl; Megakaryocyte; Mitochondria; Wnt signaling