title: Development of real-time immuno-PCR for the quantitative detection of mycobacterial PstS1 in tuberculosis patients.
abstract: A novel indirect real-time immuno-polymerase chain reaction (RT-I-PCR) assay, an evolution of I-PCR, was developed for the quantitative detection of Mycobacterium tuberculosis PstS1 (Rv0934) with a wide dynamic range of 10ng/mL to 1pg/mL in body fluids of tuberculosis (TB) patients, which may monitor the dynamics of disease.
authors.affiliation: Centre for Biotechnology, Maharshi Dayanand University (MDU), Rohtak 124001, Haryana, India.
authors.forename: Suman
authors.initials: S
authors.lastname: Sharma
authors.affiliation: Centre for Biotechnology, Maharshi Dayanand University (MDU), Rohtak 124001, Haryana, India.
authors.forename: Ankush
authors.initials: A
authors.lastname: Raj
authors.affiliation: Centre for Biotechnology, Maharshi Dayanand University (MDU), Rohtak 124001, Haryana, India.
authors.forename: Netrapal
authors.initials: N
authors.lastname: Singh
authors.affiliation: Centre for Biotechnology, Maharshi Dayanand University (MDU), Rohtak 124001, Haryana, India.
authors.forename: Bhawna
authors.initials: B
authors.lastname: Dahiya
authors.affiliation: Department of Statistics, Amity Institute of Applied Sciences, Amity University, Noida 201303, India.
authors.forename: Abhishek
authors.initials: A
authors.lastname: Sheoran
authors.affiliation: Department of TB & Respiratory Medicine, University of Health Sciences (UHS), Rohtak 124001, India.
authors.forename: Krishna B
authors.initials: KB
authors.lastname: Gupta
authors.affiliation: Centre for Biotechnology, Maharshi Dayanand University (MDU), Rohtak 124001, Haryana, India. Electronic address: pkmehta3@hotmail.com.
authors.forename: Promod K
authors.initials: PK
authors.lastname: Mehta
mesh_terms: D018528:ATP-Binding Cassette Transporters / Q000235:genetics*; D000293:Adolescent; D000328:Adult; D001426:Bacterial Proteins / Q000235:genetics*; D015373:Bacterial Typing Techniques; D016022:Case-Control Studies; D015169:Colony Count, Microbial; D004269:DNA, Bacterial / Q000302:isolation & purification; D005069:Evaluation Studies as Topic; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009169:Mycobacterium tuberculosis / Q000302:isolation & purification*; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D014376:Tuberculosis / Q000175:diagnosis* / Q000382:microbiology; D055815:Young Adult
chemical_list: D018528:ATP-Binding Cassette Transporters; D001426:Bacterial Proteins; D004269:DNA, Bacterial; C581205:PstS-1 protein, Mycobacterium tuberculosis
keywords: Diagnosis; Mycobacterium tuberculosis; PstS1; Real-time immuno-PCR; Tuberculosis