title: Screening of long non-coding RNA and TUG1 inhibits proliferation with TGF-β induction in patients with COPD.
abstract: OBJECTIVE
  To evaluate differentially expressed long noncoding RNAs (lncRNAs) and the potential role of lncRNA TUG1 in patients with chronic obstructive pulmonary disease (COPD).
  
  
  METHODS
  Total RNA was extracted from both COPD and non-COPD lung tissues, and microarray analysis was performed with 25,628 lncRNA probes and 20,106 mRNA probes. In addition, five up-regulated and five down-regulated lncRNAs were selected for identification using quantitative real-time polymerase chain reaction. COPD cell model was established by transforming growth factor β (TGF-β) treatment. Cell Counting Kit-8 assay was used to detect BEAS-2B and HFL1 cell proliferation after TUG-siRNA transfection with TGF-β treatment. In addition, the expression levels of α-SMA and fibronectin proteins were determined using Western blot in BEAS-2B and HFL1 cells after TUG-siRNA transfection with TGF-β treatment.
  
  
  RESULTS
  There were 8,376 (32.7%) differentially expressed lncRNAs and 5,094 (25.3%) differentially expressed mRNAs in COPD lung tissues compared with non-COPD lung tissues. Five of the analyzed lncRNAs (BC038205, BC130595, TUG1, MEG3, and LOC646329) were markedly increased, while five lncRNAs (LOC729178, PLAC2, LOC339529, LINC00229, and SNHG5) were significantly decreased in COPD lung tissues compared with non-COPD lung tissues (n=20) (***P<0.001). Knockdown of lncRNA TUG1 promotes BEAS-2B and HFL1 cell proliferation after TGF-β treatment through inhibiting the expression levels of α-SMA and fibronectin.
  
  
  CONCLUSIONS
  Abundant, differentially expressed lncRNAs and mRNAs were identified by microarray analysis and these might play a partial or key role in the diagnosis of patients with COPD. LncRNA TUG1 may become a very important class of biomarker and may act as a potential diagnostic and therapeutic target for patients with COPD.
authors.affiliation: Department of Respiratory Medicine, The Third Xiangya Hospital of Central South University.
authors.forename: Wenxiang
authors.initials: W
authors.lastname: Tang
authors.affiliation: Department of Respiratory Medicine, Xiangtan Central Hospital, Hunan, People's Republic of China.
authors.forename: Zhenyu
authors.initials: Z
authors.lastname: Shen
authors.affiliation: Department of Respiratory Medicine, Xiangtan Central Hospital, Hunan, People's Republic of China.
authors.forename: Jiang
authors.initials: J
authors.lastname: Guo
authors.affiliation: Department of Respiratory Medicine, The Third Xiangya Hospital of Central South University.
authors.forename: Shenghua
authors.initials: S
authors.lastname: Sun
mesh_terms: D000199:Actins / Q000235:genetics / Q000378:metabolism; D000328:Adult; D016022:Case-Control Studies; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects*; D004847:Epithelial Cells / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D005260:Female; D005347:Fibroblasts / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D005353:Fibronectins / Q000235:genetics / Q000378:metabolism; D020869:Gene Expression Profiling / Q000379:methods; D005786:Gene Expression Regulation; D006801:Humans; D008168:Lung / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D008297:Male; D008875:Middle Aged; D020411:Oligonucleotide Array Sequence Analysis; D029424:Pulmonary Disease, Chronic Obstructive / Q000175:diagnosis / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D034622:RNA Interference; D062085:RNA, Long Noncoding / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D013997:Time Factors; D014162:Transfection; D016212:Transforming Growth Factor beta / Q000494:pharmacology*
chemical_list: C541116:ACTA2 protein, human; D000199:Actins; D005353:Fibronectins; D062085:RNA, Long Noncoding; D012333:RNA, Messenger; C000598536:TUG1 long noncoding RNA, human; D016212:Transforming Growth Factor beta
keywords: COPD; TGF-β; lncRNA TUG1; long noncoding RNA; microarray analysis