title: Gene Therapy of Salivary Diseases.
abstract: For many years, our research group worked to develop gene transfer approaches for salivary gland disorders that lacked effective conventional therapy. The purpose of this chapter is to describe and update key methods used in this process. As described in our original chapter from the 2010 volume, we focus on one clinical condition, irradiation-induced salivary hypofunction, and address the choice of transgene and vector to be used, the construction of recombinant viral vectors, how vector delivery is accomplished, and methods for assessing vector function in vitro and in an appropriate animal model.
authors.affiliation: Molecular Physiology and Therapeutics Branch, National Institute of Dental and Craniofacial Research, National Institutes of Health (NIH), Bethesda, MD, 20892, USA. bbaum@dir.nidcr.nih.gov.|Molecular Physiology and Therapeutics Branch, National Institute of Dental and Craniofacial Research, National Institutes of Health (NIH), Building 10, Room 1N113, MSC-1190, Bethesda, MD, 20892-1190, USA. bbaum@dir.nidcr.nih.gov.
authors.forename: Bruce J
authors.initials: BJ
authors.lastname: Baum
authors.affiliation: Molecular Physiology and Therapeutics Branch, National Institute of Dental and Craniofacial Research, National Institutes of Health (NIH), Bethesda, MD, 20892, USA.
authors.forename: Sandra
authors.initials: S
authors.lastname: Afione
authors.affiliation: Molecular Physiology and Therapeutics Branch, National Institute of Dental and Craniofacial Research, National Institutes of Health (NIH), Bethesda, MD, 20892, USA.
authors.forename: John A
authors.initials: JA
authors.lastname: Chiorini
authors.affiliation: Molecular Physiology and Therapeutics Branch, National Institute of Dental and Craniofacial Research, National Institutes of Health (NIH), Bethesda, MD, 20892, USA.
authors.forename: Ana P
authors.initials: AP
authors.lastname: Cotrim
authors.affiliation: Molecular Physiology and Therapeutics Branch, National Institute of Dental and Craniofacial Research, National Institutes of Health (NIH), Bethesda, MD, 20892, USA.
authors.forename: Corinne M
authors.initials: CM
authors.lastname: Goldsmith
authors.affiliation: Molecular Physiology and Therapeutics Branch, National Institute of Dental and Craniofacial Research, National Institutes of Health (NIH), Bethesda, MD, 20892, USA.
authors.forename: Changyu
authors.initials: C
authors.lastname: Zheng
mesh_terms: D000260:Adenoviruses, Human / Q000235:genetics; D000818:Animals; D002460:Cell Line; D000229:Dependovirus; D004195:Disease Models, Animal; D023061:Gene Order; D018014:Gene Transfer Techniques; D015316:Genetic Therapy / Q000379:methods; D005822:Genetic Vectors / Q000008:administration & dosage / Q000235:genetics / Q000302:isolation & purification; D006801:Humans; D019219:Parvovirinae / Q000235:genetics; D012466:Salivary Gland Diseases / Q000235:genetics* / Q000628:therapy*; D012469:Salivary Glands / Q000378:metabolism; D014161:Transduction, Genetic; D019076:Transgenes
keywords: Adeno-associated virus; Adenovirus; Gene therapy; Radiation damage; Salivary glands; Salivary hypofunction