title: Clostridium guangxiense sp. nov. and Clostridium neuense sp. nov., two phylogenetically closely related hydrogen-producing species isolated from lake sediment.
abstract: Two novel anaerobic, mesophilic, biohydrogen-producing bacteria, designated strains ZGM211T and G1T, were isolated from lake sediment. 16S rRNA and ATP synthase beta subunit (atpD) gene sequences and phylogenetic analysis of strains ZGM211T and G1T revealed an affiliation to the genus Clostridium sensu stricto (cluster I of the clostridia), with Clostridium acetobutylicum as the closest characterized species, showing the same sequence similarity of 96.4 % to the type strain (98.9 % between the two isolates). Cells of the two strains were rod shaped. Growth occurred at 20-45 °C, pH 4.0-8.0 and NaCl concentrations up to 2 % (w/v). Grown on glucose, the main fermentation products were H2, CO2, acetate and butyrate. The major fatty acids were C14 : 0 and C16 : 0. The DNA G+C contents of strains ZGM211T and G1T were 40.7 and 41.5 mol%, respectively. Based on phenotypic, chemotaxonomic and phylogenetic differences, strains ZGM211T (=CICC 24070T=BCRC 80950T) and G1T (=CICC 24069T=BCRC 80949T) are proposed as the type strains of novel species of the genus Clostridium with the names Clostridium guangxiense sp. nov. and Clostridium neuense sp. nov., respectively.
authors.affiliation: Department of Environmental Engineering, School of Resource and Civil Engineering, Northeastern University, Shenyang 110004, PR China.
authors.forename: Xin
authors.initials: X
authors.lastname: Zhao
authors.affiliation: Department of Environmental Engineering, School of Resource and Civil Engineering, Northeastern University, Shenyang 110004, PR China.
authors.forename: Danyang
authors.initials: D
authors.lastname: Li
authors.affiliation: Department of Environmental Engineering, School of Resource and Civil Engineering, Northeastern University, Shenyang 110004, PR China.
authors.forename: Shuhong
authors.initials: S
authors.lastname: Xu
authors.affiliation: Department of Environmental Engineering, School of Resource and Civil Engineering, Northeastern University, Shenyang 110004, PR China.
authors.forename: Zhanghao
authors.initials: Z
authors.lastname: Guo
authors.affiliation: Department of Environmental Engineering, School of Resource and Civil Engineering, Northeastern University, Shenyang 110004, PR China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Environmental Engineering, School of Resource and Civil Engineering, Northeastern University, Shenyang 110004, PR China.
authors.forename: Lin
authors.initials: L
authors.lastname: Man
authors.affiliation: Department of Environmental Engineering, School of Resource and Civil Engineering, Northeastern University, Shenyang 110004, PR China.
authors.forename: Binhui
authors.initials: B
authors.lastname: Jiang
authors.affiliation: Department of Environmental Engineering, School of Resource and Civil Engineering, Northeastern University, Shenyang 110004, PR China.
authors.forename: Xiaomin
authors.initials: X
authors.lastname: Hu
mesh_terms: D001421:Bacteria, Anaerobic / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D015373:Bacterial Typing Techniques; D001482:Base Composition; D002681:China; D003013:Clostridium / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D005285:Fermentation; D019015:Geologic Sediments / Q000382:microbiology; D006859:Hydrogen / Q000378:metabolism; D060106:Lakes / Q000737:chemistry*; D010743:Phospholipids / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D006859:Hydrogen