title: Enzyme-Responsive Procarriers Capable of Transporting Chloride Ions across Lipid and Cellular Membranes.
abstract: Adopting the concept of procarrier for the first time, we demonstrated the controlled transport of chloride ions across lipid and cellular membranes. Procarriers containing highly hydrophilic appendages were initially inactive due to the lack of their partitioning into lipophilic membranes but were activated to transport chloride ions in the presence of specific enzymes that were able to hydrolyze off the appendages to generate an active carrier under specific conditions. Namely, the procarrier with an ester-bond-linked appendage was most activated by an esterase (PLE) at pH = 7.4, whereas the procarrier with a glycosyl-bond-linked appendage was activated only by a glycosylase (AOG) under slightly acidic conditions (pH = 5.5-6). In addition to controlling chloride transport, hydrophilic appendages greatly increase the water solubility of the procarrier, which may improve the deliverability of a hydrophobic active carrier into a plasma membrane.
authors.affiliation: Department of Chemistry, Yonsei University , Seoul 120-749, Korea.
authors.forename: Ye Rin
authors.initials: YR
authors.lastname: Choi
authors.affiliation: Department of Chemistry, Yonsei University , Seoul 120-749, Korea.
authors.forename: Bom
authors.initials: B
authors.lastname: Lee
authors.affiliation: College of Pharmacy, Yonsei International Campus , Incheon 406-840, Korea.
authors.forename: Jinhong
authors.initials: J
authors.lastname: Park
authors.affiliation: College of Pharmacy, Yonsei International Campus , Incheon 406-840, Korea.
authors.forename: Wan
authors.initials: W
authors.lastname: Namkung
authors.affiliation: Department of Chemistry, Yonsei University , Seoul 120-749, Korea.
authors.forename: Kyu-Sung
authors.initials: KS
authors.lastname: Jeong
mesh_terms: D002462:Cell Membrane / Q000737:chemistry* / Q000378:metabolism*; D002712:Chlorides / Q000737:chemistry / Q000378:metabolism*; D045647:DNA Glycosylases / Q000737:chemistry / Q000378:metabolism*; D004950:Esterases / Q000737:chemistry / Q000378:metabolism*; D057927:Hydrophobic and Hydrophilic Interactions; D007477:Ions / Q000737:chemistry / Q000378:metabolism; D015394:Molecular Structure
chemical_list: D002712:Chlorides; D007477:Ions; D004950:Esterases; D045647:DNA Glycosylases