title: Immunochromatographic determination of bacopaside I in biological samples.
abstract: We describe a novel immunochromatographic method for qualitative and quantitative analyses of bacopaside I, a bioactive constituent in Bacopa monnieri (L.) Wettst in biological samples. The assay was performed on polyethersulfone membrane using a polyclonal antibody raised against bacopaside I. The finalised method could quantitatively determine bacopaside I in the range of 31.3-1000.0ng and the detection and quantification limits were 1.0 and 31.3ng, respectively. The percentage recoveries of bacopaside I in blood and urine were nearly 100% indicating the accuracy of the extraction. The method was then applied for the determination of this compound in rat serum, urine and feces after an oral dose of 15mg/kg body weight. About 4% of the ingested dose of bacopaside I was detected in rat feces but none was detected in serum and urine which accorded with results from liquid chromatography tandem mass spectrometry. The accuracy, selectivity, sensitivity of the method are appropriate for in vivo pharmacokinetic studies.
authors.affiliation: Department of Pharmaceutical Chemistry and Pharmacognosy, Faculty of Pharmaceutical Sciences and Center of Excellence for Innovation in Chemistry, Naresuan University, Phitsanulok 65000, Thailand.
authors.forename: Sontaya
authors.initials: S
authors.lastname: Sookying
authors.affiliation: Department of Pharmacy Practice, Faculty of Pharmaceutical Sciences, Naresuan University, Phitsanulok 65000, Thailand.
authors.forename: Dumrongsak
authors.initials: D
authors.lastname: Pekthong
authors.affiliation: Department of Pharmacy Practice, Faculty of Pharmaceutical Sciences, Naresuan University, Phitsanulok 65000, Thailand.
authors.forename: Sarawut
authors.initials: S
authors.lastname: Oo-Puthinan
authors.affiliation: Faculty of Public Health, Naresuan University, Phitsanulok 65000, Thailand.
authors.forename: Watoo
authors.initials: W
authors.lastname: Phrompittayarat
authors.affiliation: Faculty of Pharmaceutical Sciences, Khon Kaen University, Khon Kaen 40002, Thailand.
authors.forename: Waraporn
authors.initials: W
authors.lastname: Putalun
authors.affiliation: Department of Medicinal Plant Breeding, Graduate School of Pharmaceutical Sciences, Kyushu University, Fukuoka 812-8582, Japan.
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Tanaka
authors.affiliation: Customer Support Centre, Shimadzu (Asia Pacific) Pte Ltd, 79 Science Park Drive, #02-01/08, Cintech IV, Singapore Science Park 1, Singapore 118264, Singapore.
authors.forename: Jie
authors.initials: J
authors.lastname: Xing
authors.affiliation: Customer Support Centre, Shimadzu (Asia Pacific) Pte Ltd, 79 Science Park Drive, #02-01/08, Cintech IV, Singapore Science Park 1, Singapore 118264, Singapore.
authors.forename: Zhaoqi
authors.initials: Z
authors.lastname: Zhan
authors.affiliation: Department of Pharmaceutical Chemistry and Pharmacognosy, Faculty of Pharmaceutical Sciences and Center of Excellence for Innovation in Chemistry, Naresuan University, Phitsanulok 65000, Thailand.
authors.forename: Nantaka
authors.initials: N
authors.lastname: Khorana
authors.affiliation: Science Laboratory Centre, Faculty of Science, Naresuan University, Phitsanulok 65000, Thailand.
authors.forename: Nitra
authors.initials: N
authors.lastname: Nuengchamnong
authors.affiliation: Department of Pharmaceutical Chemistry and Pharmacognosy, Faculty of Pharmaceutical Sciences and Center of Excellence for Innovation in Chemistry, Naresuan University, Phitsanulok 65000, Thailand. Electronic address: k_ingkaninan@yahoo.com.
authors.forename: Kornkanok
authors.initials: K
authors.lastname: Ingkaninan
mesh_terms: D000818:Animals; D055910:Antibodies, Immobilized / Q000737:chemistry; D034681:Bacopa / Q000737:chemistry; D002846:Chromatography, Affinity / Q000379:methods*; D005243:Feces / Q000737:chemistry; D057230:Limit of Detection; D008297:Male; D008567:Membranes, Artificial; D010936:Plant Extracts / Q000737:chemistry; D011108:Polymers / Q000737:chemistry; D051381:Rats; D017208:Rats, Wistar; D012503:Saponins / Q000032:analysis / Q000097:blood* / Q000652:urine*; D013450:Sulfones / Q000737:chemistry; D014315:Triterpenes / Q000032:analysis / Q000097:blood* / Q000652:urine*
chemical_list: D055910:Antibodies, Immobilized; D008567:Membranes, Artificial; D010936:Plant Extracts; D011108:Polymers; D012503:Saponins; D013450:Sulfones; D014315:Triterpenes; C022840:polyether sulfone; C442170:bacopaside I
keywords: Bacopa monnieri; Bacopaside I; Immunochromatography; Metabolism