title: Dodecylphosphocholine Micelles Induce Amyloid Formation of the PrP(110-136) Peptide via an α-Helical Metastable Conformation.
abstract: A peptide encompassing the conserved hydrophobic region and the first β-strand of the prion protein (PrP(110-136)) shown to interact with the surface of dodecylphosphocholine micelles adopts an α-helical conformation that is localized below the head-group layer. This surface-bound peptide has a half-life of one day, and readily initiates the formation of amyloid fibrils. The presence of the latter was confirmed using birefringence microscopy upon Congo red binding and thioflavin T-binding induced fluorescence. The observation of this metastable α-helical conformer provides a unique snapshot of the early steps of the inter-conversion pathway. These findings together with the body of evidence from the prion literature allowed us to propose a mechanism for the conversion of PrPC to amyloid material.
authors.affiliation: Centre for Biologics Evaluation, Biologics and Genetic Therapies Directorate, Health Canada, Ottawa, Ontario, Canada.
authors.forename: Simon
authors.initials: S
authors.lastname: Sauvé
authors.affiliation: Centre for Biologics Evaluation, Biologics and Genetic Therapies Directorate, Health Canada, Ottawa, Ontario, Canada.|Department of Chemistry, Carleton University, Ottawa, Ontario, Canada.
authors.forename: Yves
authors.identifier: http://orcid.org/0000-0002-8554-0297
authors.initials: Y
authors.lastname: Aubin
mesh_terms: D000682:Amyloid / Q000378:metabolism*; D052160:Benzothiazoles; D001718:Birefringence; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D008823:Micelles; D008853:Microscopy; D010767:Phosphorylcholine / Q000031:analogs & derivatives* / Q000494:pharmacology; D000072002:Prion Proteins / Q000096:biosynthesis* / Q000737:chemistry; D000072756:Protein Conformation, alpha-Helical; D013844:Thiazoles / Q000378:metabolism
chemical_list: D000682:Amyloid; D052160:Benzothiazoles; D008823:Micelles; D000072002:Prion Proteins; D013844:Thiazoles; D010767:Phosphorylcholine; C009462:thioflavin T; C028810:dodecylphosphocholine