title: Differential procoagulant activity of microparticles derived from monocytes, granulocytes, platelets and endothelial cells: impact of active tissue factor.
abstract: : Microparticles released by activated/apoptotic cells exhibit coagulation activity as they express phosphatidylserine and some of them - tissue factor. We compared procoagulant properties of microparticles from monocytes, granulocytes, platelets and endothelial cells and assessed the impact of tissue factor in observed differences. Microparticles were sedimented (20 000g, 30 min) from the supernatants of activated monocytes, monocytic THP-1 cells, granulocytes, platelets and endothelial cells. Coagulation activity of microparticles was examined using plasma recalcification assay. The size of microparticles was evaluated by dynamic light scattering. Tissue factor activity was measured by its ability to activate factor X. All microparticles significantly accelerated plasma coagulation with the shortest lag times for microparticles derived from monocytes, intermediate - for microparticles from THP-1 cells and endothelial cells, and the longest - for microparticles from granulocytes and platelets. Average diameters of microparticles ranged within 400-600 nm. The largest microparticles were produced by endothelial cells and granulocytes, smaller - by monocytes, and the smallest - by THP-1 cells and platelets. The highest tissue factor activity was detected in microparticles from monocytes, lower activity - in microparticles from endothelial cells and THP-1 cells, and no activity - in microparticles from platelets and granulocytes. Anti-tissue factor antibodies extended coagulation lag times for microparticles from monocytes, endothelial cells and THP-1 cells and equalized them with those for microparticles from platelets and granulocytes. Higher coagulation activity of microparticles from monocytes, THP-1 cells and endothelial cells in comparison with microparticles from platelets and granulocytes is determined mainly by the presence of active tissue factor.
authors.affiliation: aRussian Cardiology Research and Production Complex, Russian Ministry of Health bInstitute of Information Systems, State University of Management cInstitute of General Pathology and Pathophysiology, Moscow, Russian Federation.
authors.forename: Olga N
authors.initials: ON
authors.lastname: Shustova
authors.forename: Olga A
authors.initials: OA
authors.lastname: Antonova
authors.forename: Nina V
authors.initials: NV
authors.lastname: Golubeva
authors.forename: Svetlana G
authors.initials: SG
authors.lastname: Khaspekova
authors.forename: Vladimir V
authors.initials: VV
authors.lastname: Yakushkin
authors.forename: Svetlana A
authors.initials: SA
authors.lastname: Aksuk
authors.forename: Irina B
authors.initials: IB
authors.lastname: Alchinova
authors.forename: Mikhail Y
authors.initials: MY
authors.lastname: Karganov
authors.forename: Alexey V
authors.initials: AV
authors.lastname: Mazurov
mesh_terms: D001777:Blood Coagulation; D001792:Blood Platelets / Q000378:metabolism*; D002460:Cell Line; D055252:Cell-Derived Microparticles / Q000378:metabolism*; D042783:Endothelial Cells / Q000378:metabolism*; D006098:Granulocytes / Q000378:metabolism*; D006801:Humans; D009000:Monocytes / Q000378:metabolism*; D010316:Particle Size; D013925:Thromboplastin / Q000378:metabolism*
chemical_list: D013925:Thromboplastin