title: Proteomics Analysis of Blood Serums from Alzheimer's Disease Patients Using iTRAQ Labeling Technology.
abstract: Alzheimer' disease (AD) is the most common form of dementia affecting up to 6% of the population over the age of 65. In order to discover differentially expressed proteins that might serve as potential biomarkers, the serums from AD patients and healthy controls were compared and analyzed using the proteomics approach of isobaric tagging for relative and absolute quantitation (iTRAQ). For the first time, AD biomarkers in serums are investigated in the Han Chinese population using iTRAQ labeled proteomics strategy. Twenty-two differentially expressed proteins were identified and out of which nine proteins were further validated with more sample test. Another three proteins that have been reported in the literature to be potentially associated with AD were also investigated for alteration in expression level. Functions of those proteins were mainly related to the following processes: amyloid-β (Aβ) metabolism, cholesterol transport, complement and coagulation cascades, immune response, inflammation, hemostasis, hyaluronan metabolism, and oxidative stress. These results support current views on the molecular mechanism of AD. For the first time, differential expression of zinc-alpha-2-glycoprotein (AZGP1), fibulin-1 (FBLN1), platelet basic protein (PPBP), thrombospondin-1 (THBS1), S100 calcium-binding protein A8 (S100A8), and S100 calcium-binding protein A9 (S100A9) were detected in the serums of AD patients compared with healthy controls. These proteins might play a role in AD pathophysiology and serve as potential biomarkers for AD diagnosis. Specifically, our results strengthened the crucial role of Aβ metabolism and blood coagulation in AD pathogenesis and proteins related to these two processes may be used as peripheral blood biomarkers for AD.
authors.affiliation: College of Life Science and Oceanography, Shenzhen Key Laboratory of Marine Biotechnology and Ecology, Shenzhen University, Shenzhen, P.R. China.
authors.forename: Liming
authors.initials: L
authors.lastname: Shen
authors.affiliation: College of Life Science and Oceanography, Shenzhen Key Laboratory of Marine Biotechnology and Ecology, Shenzhen University, Shenzhen, P.R. China.
authors.forename: Liping
authors.initials: L
authors.lastname: Liao
authors.affiliation: College of Life Science and Oceanography, Shenzhen Key Laboratory of Marine Biotechnology and Ecology, Shenzhen University, Shenzhen, P.R. China.
authors.forename: Cheng
authors.initials: C
authors.lastname: Chen
authors.affiliation: Department of Neurology, Shenzhen People's Hospital, P.R. China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Guo
authors.affiliation: Department of Geriatrics, Qingdao Municipal Hospital, Qingdao, P.R. China.
authors.forename: Dalin
authors.initials: D
authors.lastname: Song
authors.affiliation: College of Life Science and Oceanography, Shenzhen Key Laboratory of Marine Biotechnology and Ecology, Shenzhen University, Shenzhen, P.R. China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Wang
authors.affiliation: College of Life Science and Oceanography, Shenzhen Key Laboratory of Marine Biotechnology and Ecology, Shenzhen University, Shenzhen, P.R. China.
authors.forename: Youjiao
authors.initials: Y
authors.lastname: Chen
authors.affiliation: College of Life Science and Oceanography, Shenzhen Key Laboratory of Marine Biotechnology and Ecology, Shenzhen University, Shenzhen, P.R. China.
authors.forename: Kaoyuan
authors.initials: K
authors.lastname: Zhang
authors.affiliation: College of Life Science and Oceanography, Shenzhen Key Laboratory of Marine Biotechnology and Ecology, Shenzhen University, Shenzhen, P.R. China.
authors.forename: Ming
authors.initials: M
authors.lastname: Ying
authors.affiliation: College of Life Science and Oceanography, Shenzhen Key Laboratory of Microbial Genetic Engineering, Shenzhen University, Shenzhen, P.R. China.
authors.forename: Shuiming
authors.initials: S
authors.lastname: Li
authors.affiliation: College of Life Science and Oceanography, Shenzhen Key Laboratory of Marine Biotechnology and Ecology, Shenzhen University, Shenzhen, P.R. China.
authors.forename: Qiong
authors.initials: Q
authors.lastname: Liu
authors.affiliation: College of Life Science and Oceanography, Shenzhen Key Laboratory of Marine Biotechnology and Ecology, Shenzhen University, Shenzhen, P.R. China.
authors.forename: Jiazuan
authors.initials: J
authors.lastname: Ni
mesh_terms: D054392:Adipokines; D000544:Alzheimer Disease / Q000097:blood* / Q000000981:diagnostic imaging; D044466:Asian People / Q000208:ethnology; D015415:Biomarkers / Q000097:blood*; D002135:Calcium-Binding Proteins / Q000097:blood; D040501:Calgranulin A / Q000097:blood; D040502:Calgranulin B / Q000097:blood; D002352:Carrier Proteins / Q000097:blood; D016022:Case-Control Studies; D019295:Computational Biology; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006023:Glycoproteins / Q000097:blood; D006801:Humans; D008297:Male; D049268:Positron-Emission Tomography; D060066:Protein Interaction Maps; D040901:Proteomics / Q000379:methods*; D064568:S100 Calcium Binding Protein beta Subunit / Q000097:blood; D019700:Thrombospondin 1 / Q000097:blood; D001620:beta-Thromboglobulin / Q000378:metabolism; D000090203:Zn-Alpha-2-Glycoprotein
chemical_list: D054392:Adipokines; D015415:Biomarkers; D002135:Calcium-Binding Proteins; D040501:Calgranulin A; D040502:Calgranulin B; D002352:Carrier Proteins; D006023:Glycoproteins; D064568:S100 Calcium Binding Protein beta Subunit; D019700:Thrombospondin 1; D001620:beta-Thromboglobulin; C488891:AZGP1 protein, human; D000090203:Zn-Alpha-2-Glycoprotein; C061615:PPBP protein, human; C579160:S100B protein, human; C060820:fibulin; C000611726:thrombospondin-1, human
keywords: Alzheimer’ disease; biomarker; iTRAQ; proteomics; serum