title: Characterization of the first double-stranded RNA bacteriophage infecting Pseudomonas aeruginosa.
abstract: Bacteriophages (phages) are widely distributed in the biosphere and play a key role in modulating microbial ecology in the soil, ocean, and humans. Although the role of DNA bacteriophages is well described, the biology of RNA bacteriophages is poorly understood. More than 1900 phage genomes are currently deposited in NCBI, but only 6 dsRNA bacteriophages and 12 ssRNA bacteriophages genome sequences are reported. The 6 dsRNA bacteriophages were isolated from legume samples or lakes with Pseudomonas syringae as the host. Here, we report the first Pseudomonas aeruginosa phage phiYY with a three-segmented dsRNA genome. phiYY was isolated from hospital sewage in China with the clinical P. aeruginosa strain, PAO38, as a host. Moreover, the dsRNA phage phiYY has a broad host range, which infects 99 out of 233 clinical P. aeruginosa strains isolated from four provinces in China. This work presented a detailed characterization of the dsRNA bacteriophage infecting P. aeruginosa.
authors.affiliation: Department of Microbiology, Third Military Medical University, Chongqing, 400038, China.
authors.forename: Yuhui
authors.initials: Y
authors.lastname: Yang
authors.affiliation: Department of Microbiology, Third Military Medical University, Chongqing, 400038, China.
authors.forename: Shuguang
authors.initials: S
authors.lastname: Lu
authors.affiliation: Department of Microbiology, Third Military Medical University, Chongqing, 400038, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Shen
authors.affiliation: Department of Microbiology, Third Military Medical University, Chongqing, 400038, China.
authors.forename: Xia
authors.initials: X
authors.lastname: Zhao
authors.affiliation: Department of Microbiology, Third Military Medical University, Chongqing, 400038, China.
authors.forename: Mengyu
authors.initials: M
authors.lastname: Shen
authors.affiliation: Department of Microbiology, Third Military Medical University, Chongqing, 400038, China.
authors.forename: Yinling
authors.initials: Y
authors.lastname: Tan
authors.affiliation: Department of Microbiology, Third Military Medical University, Chongqing, 400038, China.
authors.forename: Gang
authors.initials: G
authors.lastname: Li
authors.affiliation: Department of Microbiology, Third Military Medical University, Chongqing, 400038, China.
authors.forename: Ming
authors.initials: M
authors.lastname: Li
authors.affiliation: Department of Microbiology, Third Military Medical University, Chongqing, 400038, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Wang
authors.affiliation: Department of Microbiology, Third Military Medical University, Chongqing, 400038, China.
authors.forename: Fuquan
authors.initials: F
authors.lastname: Hu
authors.affiliation: Department of Microbiology, Third Military Medical University, Chongqing, 400038, China.
authors.forename: Shuai
authors.initials: S
authors.lastname: Le
mesh_terms: D015373:Bacterial Typing Techniques; D001435:Bacteriophages / Q000235:genetics / Q000302:isolation & purification*; D002681:China; D003428:Cross Infection / Q000382:microbiology; D016679:Genome, Viral; D058507:Host Specificity; D006801:Humans; D008506:Medical Waste; D016366:Open Reading Frames; D010802:Phylogeny; D011552:Pseudomonas Infections / Q000382:microbiology; D011550:Pseudomonas aeruginosa / Q000302:isolation & purification / Q000821:virology*; D012330:RNA, Double-Stranded / Q000302:isolation & purification; D012367:RNA, Viral / Q000302:isolation & purification; D012722:Sewage / Q000821:virology
chemical_list: D008506:Medical Waste; D012330:RNA, Double-Stranded; D012367:RNA, Viral; D012722:Sewage