title: Protein profiles of Taenia solium cysts obtained from skeletal muscles and the central nervous system of pigs: Search for tissue-specific proteins.
abstract: Taeniasis/cysticercosis caused by the tapeworm Taenia solium is a parasite disease transmitted among humans and pigs, the main intermediate host. The larvae/cysts can lodge in several tissues of the pig, i.e. skeletal muscles and different locations of the central nervous system. The molecular mechanisms associated to tissue preferences of the cysts remain poorly understood. The major public health concern about this zoonosis is due to the human infections by the larval form in the central nervous system, causing a highly pleomorphic and debilitating disease known as neurocysticercosis. This study was aimed to explore the 2DE protein maps of T. solium cysts obtained from skeletal muscles and central nervous system of naturally infected pigs. The gel images were analyzed through a combination of PDQuest™ and multivariate analysis. Results showed that differences in the protein patterns of cysts obtained from both tissues were remarkably discrete. Only 7 protein spots were found specifically associated to the skeletal muscle localization of the cysts; none was found significantly associated to the central nervous system. The use of distinct protein fractions of cysts allowed preliminary identification of several tissue-specific antigenic bands. The implications of these findings are discussed, as well as several strategies directed to achieve the complete characterization of this parasite's proteome, in order to extend our understanding of the molecular mechanisms underlying tissue localization of the cysts and to open avenues for the development of immunological tissue-specific diagnosis of the disease.
authors.affiliation: Dept. of Immunology, Institute for Biomedical Research, Universidad Nacional Autónoma de México, México D.F, 04510, Mexico.
authors.forename: José
authors.initials: J
authors.lastname: Navarrete-Perea
authors.affiliation: Dept. of Immunology, Institute for Biomedical Research, Universidad Nacional Autónoma de México, México D.F, 04510, Mexico.
authors.forename: Bárbara
authors.initials: B
authors.lastname: Moguel
authors.affiliation: Dept. of Immunology, Institute for Biomedical Research, Universidad Nacional Autónoma de México, México D.F, 04510, Mexico.
authors.forename: Raúl José
authors.initials: RJ
authors.lastname: Bobes
authors.affiliation: Facultad de Medicina Veterinaria y Zootecnia, Universidad Nacional Autónoma de México, México D.F, 04510, Mexico.
authors.forename: Nelly
authors.initials: N
authors.lastname: Villalobos
authors.affiliation: Dept. of Immunology, Institute for Biomedical Research, Universidad Nacional Autónoma de México, México D.F, 04510, Mexico.
authors.forename: Julio César
authors.initials: JC
authors.lastname: Carrero
authors.affiliation: Dept. of Immunology, Institute for Biomedical Research, Universidad Nacional Autónoma de México, México D.F, 04510, Mexico.
authors.forename: Edda
authors.initials: E
authors.lastname: Sciutto
authors.affiliation: Instituto Nacional de Medicina Genómica, Periférico Sur No. 4809, Col. Arenal Tepepan, Delegación Tlalpan, México, D.F, C.P. 14610, Mexico; Instituto de Biotecnología, Universidad Nacional Autónoma de México, Avenida Universidad 2001, Cuernavaca, Morelos, C.P. 62210, Mexico.
authors.forename: Xavier
authors.initials: X
authors.lastname: Soberón
authors.affiliation: Dept. of Immunology, Institute for Biomedical Research, Universidad Nacional Autónoma de México, México D.F, 04510, Mexico. Electronic address: laclette@biomedicas.unam.mx.
authors.forename: Juan Pedro
authors.initials: JP
authors.lastname: Laclette
mesh_terms: D000818:Animals; D001921:Brain / Q000469:parasitology*; D003551:Cysticercosis / Q000469:parasitology / Q000662:veterinary*; D003552:Cysticercus / Q000737:chemistry* / Q000302:isolation & purification; D015180:Electrophoresis, Gel, Two-Dimensional; D015801:Helminth Proteins / Q000032:analysis*; D018482:Muscle, Skeletal / Q000469:parasitology*; D034421:Sus scrofa; D013552:Swine; D013553:Swine Diseases / Q000469:parasitology*; D041201:Taenia solium / Q000737:chemistry* / Q000302:isolation & purification
chemical_list: D015801:Helminth Proteins
keywords: 2D-PAGE; Canonical discriminant analysis; Cysticercosis; Principal component analysis; Tissue-specific antigens