title: Differential expression of Low density lipoprotein Receptor-related Protein 1 (LRP-1) and matrix metalloproteinase-9 (MMP-9) in prostate gland: From normal to malignant lesions.
abstract: BACKGROUND
  Metalloproteinases (MMPs) are relevant modulators of inflammation, tumor microenvironment, cancer invasion and metastasis. They can be regulated by the Low density lipoprotein Receptor-related Protein 1 (LRP-1), a receptor reported to mediate the clearance of lipoproteins, extracellular matrix (ECM) macromolecules and proteinases. The aim of this study was to evaluate the expression of LRP-1, MMP-2 and MMP-9 across various grades of prostatic diseases as benign prostatic hyperplasia (BPH), BPH plus prostatitis (BPH+P), high grade prostatic intraepithelial neoplasia (HGPIN) and prostate cancer (PCa).
  
  
  METHODS
  LRP-1 was analyzed using immunohistochemistry and MMPs proteolytic activity by zymography in prostate tissues with different prostatic diseases.
  
  
  RESULTS
  LRP-1 was detected in epithelial cells in BPH (16/18), BPH+P (21/21) and HGPIN (6/6), with a staining intensity of 1+, 1+/2+ and 3+, respectively. In PCa, LRP-1 was absent in 19/27 samples while a low expression was observed in 8/27 biopsies. MMP-9 activity was higher and statistically significant in PCa than in BPH (p≤0.01).
  
  
  CONCLUSIONS
  Considering that LRP-1, by mediating the clearance of MMPs, is involved in the regulation of ECM remodeling and cell migration, we conclude that a decreased expression of LRP-1 could be involved with the increasing activity of MMPs shown in cancers.
authors.affiliation: Centro de Investigaciones en Bioquímica Clínica e Inmunología, CONICET, Departamento de Bioquímica Clínica, FCQ-UNC, Córdoba, Argentina. Electronic address: mgilard@fcq.unc.edu.ar.
authors.forename: Mónica B
authors.initials: MB
authors.lastname: Gilardoni
authors.affiliation: Centro de Investigaciones en Bioquímica Clínica e Inmunología, CONICET, Departamento de Bioquímica Clínica, FCQ-UNC, Córdoba, Argentina.
authors.forename: María M
authors.initials: MM
authors.lastname: Remedi
authors.affiliation: Histología y Patología, Facultad de Ciencias Médicas, UNC, Córdoba, Argentina.
authors.forename: Mabel
authors.initials: M
authors.lastname: Oviedo
authors.affiliation: Fundación Urológica Córdoba para la Docencia e Investigación Médica, FUCDIM, Córdoba, Argentina.
authors.forename: Tristán
authors.initials: T
authors.lastname: Dellavedova
authors.affiliation: Fundación Urológica Córdoba para la Docencia e Investigación Médica, FUCDIM, Córdoba, Argentina.
authors.forename: Juan P
authors.initials: JP
authors.lastname: Sarría
authors.affiliation: Fundación Urológica Córdoba para la Docencia e Investigación Médica, FUCDIM, Córdoba, Argentina.
authors.forename: Laura
authors.initials: L
authors.lastname: Racca
authors.affiliation: Fundación Urológica Córdoba para la Docencia e Investigación Médica, FUCDIM, Córdoba, Argentina.
authors.forename: Mariana
authors.initials: M
authors.lastname: Dominguez
authors.affiliation: Centro de Investigaciones en Bioquímica Clínica e Inmunología, CONICET, Departamento de Bioquímica Clínica, FCQ-UNC, Córdoba, Argentina.
authors.forename: Claudia G
authors.initials: CG
authors.lastname: Pellizas
authors.affiliation: Centro de Investigaciones en Bioquímica Clínica e Inmunología, CONICET, Departamento de Bioquímica Clínica, FCQ-UNC, Córdoba, Argentina.
authors.forename: Ana C
authors.initials: AC
authors.lastname: Donadio
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D006801:Humans; D026503:Low Density Lipoprotein Receptor-Related Protein-1 / Q000378:metabolism*; D008297:Male; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism*; D008875:Middle Aged; D011467:Prostate / Q000378:metabolism* / Q000473:pathology; D011470:Prostatic Hyperplasia / Q000378:metabolism* / Q000473:pathology; D019048:Prostatic Intraepithelial Neoplasia / Q000378:metabolism* / Q000473:pathology; D011471:Prostatic Neoplasms / Q000378:metabolism* / Q000473:pathology; D011472:Prostatitis / Q000378:metabolism* / Q000473:pathology
chemical_list: C475476:LRP1 protein, human; D026503:Low Density Lipoprotein Receptor-Related Protein-1; D020780:Matrix Metalloproteinase 9
keywords: Cancer; Immunohistochemistry; LRP-1; MMP-9; Prostate; Zymography