title: Receptor kinase complex transmits RALF peptide signal to inhibit root growth in Arabidopsis.
abstract: A number of hormones work together to control plant cell growth. Rapid Alkalinization Factor 1 (RALF1), a plant-derived small regulatory peptide, inhibits cell elongation through suppression of rhizosphere acidification in plants. Although a receptor-like kinase, FERONIA (FER), has been shown to act as a receptor for RALF1, the signaling mechanism remains unknown. In this study, we identified a receptor-like cytoplasmic kinase (RPM1-induced protein kinase, RIPK), a plasma membrane-associated member of the RLCK-VII subfamily, that is recruited to the receptor complex through interacting with FER in response to RALF1. RALF1 triggers the phosphorylation of both FER and RIPK in a mutually dependent manner. Genetic analysis of the fer-4 and ripk mutants reveals RIPK, as well as FER, to be required for RALF1 response in roots. The RALF1-FER-RIPK interactions may thus represent a mechanism for peptide signaling in plants.
authors.affiliation: Hunan Key Laboratory of Plant Functional Genomics and Developmental Regulation, Hunan University, Changsha 410082, P.R. China.
authors.forename: Changqing
authors.initials: C
authors.lastname: Du
authors.affiliation: Hunan Key Laboratory of Plant Functional Genomics and Developmental Regulation, Hunan University, Changsha 410082, P.R. China.
authors.forename: Xiushan
authors.initials: X
authors.lastname: Li
authors.affiliation: Hunan Key Laboratory of Plant Functional Genomics and Developmental Regulation, Hunan University, Changsha 410082, P.R. China.
authors.forename: Jia
authors.initials: J
authors.lastname: Chen
authors.affiliation: Hunan Key Laboratory of Plant Functional Genomics and Developmental Regulation, Hunan University, Changsha 410082, P.R. China.
authors.forename: Weijun
authors.initials: W
authors.lastname: Chen
authors.affiliation: Hunan Key Laboratory of Plant Functional Genomics and Developmental Regulation, Hunan University, Changsha 410082, P.R. China.
authors.forename: Bin
authors.initials: B
authors.lastname: Li
authors.affiliation: Hunan Key Laboratory of Plant Functional Genomics and Developmental Regulation, Hunan University, Changsha 410082, P.R. China.
authors.forename: Chiyu
authors.initials: C
authors.lastname: Li
authors.affiliation: Hunan Key Laboratory of Plant Functional Genomics and Developmental Regulation, Hunan University, Changsha 410082, P.R. China.
authors.forename: Long
authors.initials: L
authors.lastname: Wang
authors.affiliation: Hunan Key Laboratory of Plant Functional Genomics and Developmental Regulation, Hunan University, Changsha 410082, P.R. China.
authors.forename: Jianglin
authors.initials: J
authors.lastname: Li
authors.affiliation: Hunan Key Laboratory of Plant Functional Genomics and Developmental Regulation, Hunan University, Changsha 410082, P.R. China.
authors.forename: Xiaoying
authors.initials: X
authors.lastname: Zhao
authors.affiliation: Hunan Key Laboratory of Plant Functional Genomics and Developmental Regulation, Hunan University, Changsha 410082, P.R. China.
authors.forename: Jianzhong
authors.initials: J
authors.lastname: Lin
authors.affiliation: Hunan Key Laboratory of Plant Functional Genomics and Developmental Regulation, Hunan University, Changsha 410082, P.R. China; sluan@berkeley.edu xml05@hnu.edu.cn feng_yu@hnu.edu.cn.
authors.forename: Xuanming
authors.initials: X
authors.lastname: Liu
authors.affiliation: Department of Plant and Microbial Biology, University of California, Berkeley, CA 94720 sluan@berkeley.edu xml05@hnu.edu.cn feng_yu@hnu.edu.cn.
authors.forename: Sheng
authors.initials: S
authors.lastname: Luan
authors.affiliation: Hunan Key Laboratory of Plant Functional Genomics and Developmental Regulation, Hunan University, Changsha 410082, P.R. China; sluan@berkeley.edu xml05@hnu.edu.cn feng_yu@hnu.edu.cn.
authors.forename: Feng
authors.initials: F
authors.lastname: Yu
mesh_terms: D017360:Arabidopsis / Q000235:genetics / Q000254:growth & development*; D029681:Arabidopsis Proteins / Q000502:physiology*; D003593:Cytoplasm / Q000378:metabolism; D008024:Ligands; D018613:Microscopy, Confocal; D009154:Mutation; D036361:Peptide Hormones / Q000502:physiology*; D010641:Phenotype; D010766:Phosphorylation; D010770:Phosphotransferases / Q000502:physiology; D017440:Photoperiod; D010802:Phylogeny; D010937:Plant Growth Regulators / Q000502:physiology; D018517:Plant Roots / Q000254:growth & development*; D030821:Plants, Genetically Modified / Q000235:genetics / Q000254:growth & development; D000072417:Protein Domains; D011494:Protein Kinases / Q000502:physiology*; D012639:Seeds / Q000378:metabolism; D015398:Signal Transduction; D017346:Protein Serine-Threonine Kinases
chemical_list: D029681:Arabidopsis Proteins; D008024:Ligands; D036361:Peptide Hormones; D010770:Phosphotransferases; D010937:Plant Growth Regulators; D011494:Protein Kinases; C529880:RALF1 protein, Arabidopsis; C522131:At3g51550 protein, Arabidopsis; D017346:Protein Serine-Threonine Kinases; C558451:RPM1-induced protein kinase, Arabidopsis
keywords: feronia; phosphorylation; plant hormone