title: Label-Free Detection of Human Glycoprotein (CgA) Using an Extended-Gated Organic Transistor-Based Immunosensor.
abstract: Herein, we report on the fabrication of an extended-gated organic field-effect transistor (OFET)-based immunosensor and its application in the detection of human chromogranin A (hCgA). The fabricated OFET device possesses an extended-gate electrode immobilized with an anti-CgA antibody. The titration results of hCgA showed that the electrical changes in the OFET characteristics corresponded to the glycoprotein recognition ability of the monoclonal antibody (anti-CgA). The observed sensitivity (detection limit: 0.11 µg/mL) and selectivity indicate that the OFET-based immunosensor can be potentially applied to the rapid detection of the glycoprotein concentration without any labeling.
authors.affiliation: Research Center for Organic Electronics, Graduate School of Science and Engineering, Yamagata University, 4-3-16 Jonan, Yonezawa, Yamagata 992-8510, Japan. tey14898@st.yamagata-u.ac.jp.|Japan Society for the Promotion of Science (JSPS), Ichibancho, Chiyoda-ku, Tokyo 102-8471, Japan. tey14898@st.yamagata-u.ac.jp.|Institute of Industrial Science, The University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo 153-8505, Japan. tey14898@st.yamagata-u.ac.jp.
authors.forename: Tsukuru
authors.initials: T
authors.lastname: Minamiki
authors.affiliation: Research Center for Organic Electronics, Graduate School of Science and Engineering, Yamagata University, 4-3-16 Jonan, Yonezawa, Yamagata 992-8510, Japan. tminami@iis.u-tokyo.ac.jp.|Institute of Industrial Science, The University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo 153-8505, Japan. tminami@iis.u-tokyo.ac.jp.
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Minami
authors.affiliation: Research Center for Organic Electronics, Graduate School of Science and Engineering, Yamagata University, 4-3-16 Jonan, Yonezawa, Yamagata 992-8510, Japan. txe37183@st.yamagata-u.ac.jp.|Institute of Industrial Science, The University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo 153-8505, Japan. txe37183@st.yamagata-u.ac.jp.
authors.forename: Yui
authors.initials: Y
authors.lastname: Sasaki
authors.affiliation: Biomedical Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), 1-8-31 Midorigaoka, Ikeda, Osaka 563-8577, Japan. s.wakida@aist.go.jp.
authors.forename: Shin-Ichi
authors.initials: SI
authors.lastname: Wakida
authors.affiliation: Biomedical Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba Central 6, 1-1-1 Higashi, Tsukuba, Ibaraki 305-8566, Japan. r.kurita@aist.go.jp.
authors.forename: Ryoji
authors.initials: R
authors.lastname: Kurita
authors.affiliation: Advanced Science Research Laboratory, Saitama Institute of Technology, Fukaya, Saitama 369-0293, Japan. niwa@sit.ac.jp.
authors.forename: Osamu
authors.initials: O
authors.lastname: Niwa
authors.affiliation: Research Center for Organic Electronics, Graduate School of Science and Engineering, Yamagata University, 4-3-16 Jonan, Yonezawa, Yamagata 992-8510, Japan. tokito@yz.yamagata-u.ac.jp.
authors.forename: Shizuo
authors.initials: S
authors.lastname: Tokito
mesh_terms: D000911:Antibodies, Monoclonal / Q000737:chemistry / Q000276:immunology; D015374:Biosensing Techniques / Q000379:methods*; D053379:Chromogranin A / Q000737:chemistry / Q000276:immunology; D006023:Glycoproteins / Q000032:analysis* / Q000737:chemistry; D006801:Humans; D009930:Organic Chemicals / Q000737:chemistry*; D014173:Transistors, Electronic
chemical_list: D000911:Antibodies, Monoclonal; D053379:Chromogranin A; D006023:Glycoproteins; D009930:Organic Chemicals
keywords: antibody immobilization; chromogranin A; extended-gate; glycoprotein; immunosensor; label-free; organic field-effect transistor; self-assembled monolayer