title: Quantitative analysis of 3-OH oxylipins in fermentation yeast.
abstract: Despite the ubiquitous distribution of oxylipins in plants, animals, and microbes, and the application of numerous analytical techniques to study these molecules, 3-OH oxylipins have never been quantitatively assayed in yeasts. The formation of heptafluorobutyrate methyl ester derivatives and subsequent analysis with gas chromatography - negative chemical ionization - mass spectrometry allowed for the first determination of yeast 3-OH oxylipins. The concentration of 3-OH 10:0 (0.68-4.82 ng/mg dry cell mass) in the SMA strain of Saccharomyces pastorianus grown in laboratory-scale beverage fermentations was elevated relative to oxylipin concentrations in plant tissues and macroalgae. In fermenting yeasts, the onset of 3-OH oxylipin formation has been related to fermentation progression and flocculation initiation. When the SMA strain was grown in laboratory-scale fermentations, the maximal sugar consumption rate preceded the lowest concentration of 3-OH 10:0 by ∼4.5 h and a distinct increase in 3-OH 10:0 concentration by ∼16.5 h.
authors.affiliation: Process Engineering and Applied Science, Canadian Institute of Fermentation Technology, Dalhousie University, Halifax, NS B3J 2X4, Canada.|Process Engineering and Applied Science, Canadian Institute of Fermentation Technology, Dalhousie University, Halifax, NS B3J 2X4, Canada.
authors.forename: Greg
authors.initials: G
authors.lastname: Potter
authors.affiliation: Process Engineering and Applied Science, Canadian Institute of Fermentation Technology, Dalhousie University, Halifax, NS B3J 2X4, Canada.|Process Engineering and Applied Science, Canadian Institute of Fermentation Technology, Dalhousie University, Halifax, NS B3J 2X4, Canada.
authors.forename: Wei
authors.initials: W
authors.lastname: Xia
authors.affiliation: Process Engineering and Applied Science, Canadian Institute of Fermentation Technology, Dalhousie University, Halifax, NS B3J 2X4, Canada.|Process Engineering and Applied Science, Canadian Institute of Fermentation Technology, Dalhousie University, Halifax, NS B3J 2X4, Canada.
authors.forename: Suzanne M
authors.initials: SM
authors.lastname: Budge
authors.affiliation: Process Engineering and Applied Science, Canadian Institute of Fermentation Technology, Dalhousie University, Halifax, NS B3J 2X4, Canada.|Process Engineering and Applied Science, Canadian Institute of Fermentation Technology, Dalhousie University, Halifax, NS B3J 2X4, Canada.
authors.forename: R Alex
authors.initials: RA
authors.lastname: Speers
mesh_terms: D000818:Animals; D005285:Fermentation; D054883:Oxylipins / Q000032:analysis*; D012441:Saccharomyces cerevisiae / Q000254:growth & development / Q000378:metabolism*
chemical_list: D054883:Oxylipins
keywords: acides gras hydroxylés; fermentation miniature; heptafluorobutyrate; hydroxy fatty acids; ionisation chimique négative; mass spectrometry; miniature fermentation; negative chemical ionization; spectrométrie de masse