title: Oleate promotes differentiation of chicken primary preadipocytes in vitro.
abstract: In addition to providing energy and constituting cell membrane, fatty acids also play an important role in adipocyte differentiation and lipid metabolism. As an important member of monounsaturated fatty acids, oleate, together with other components, is widely used to induce chicken preadipocyte differentiation. However, it is not clear whether oleate alone can induce chicken preadipocyte differentiation. In the present study, four different treatments were designed to test this question: basal medium, IDX [insulin, dexamethasone and IBMX (isobutylmethylxanthine)], oleate and IDX plus oleate. Cytoplasmic lipid droplet accumulation and mRNA expression for adipogenesis-related genes were monitored. After treatment of oleate on chicken preadipocytes, apparent lipid droplet formation and lipid accumulation were observed, accompanied by increasing expression of PPARγ (peroxisome proliferator-activated receptor-γ) and AFABP (adipocyte fatty acid-binding protein), but decreasing level of GATA2 (GATA-binding protein 2). In contrast, for cells cultured in the basal medium with or without IDX supplementation, lipid droplet barely occurred. These results suggest that exogenous oleate alone can act as an inducer of preadipocyte differentiation into adipocytes.
authors.affiliation: Key Laboratory of Chicken Genetics and Breeding, Ministry of Agriculture, Harbin 150030, People's Republic of China.|Key Laboratory of Animal Genetics, Breeding and Reproduction, Education Department of Heilongjiang Province, Harbin 150030, People's Republic of China.|College of Animal Science and Technology, Northeast Agricultural University, Harbin 150030, People's Republic of China.
authors.forename: Zhouchun
authors.initials: Z
authors.lastname: Shang
authors.affiliation: Key Laboratory of Chicken Genetics and Breeding, Ministry of Agriculture, Harbin 150030, People's Republic of China.|Key Laboratory of Animal Genetics, Breeding and Reproduction, Education Department of Heilongjiang Province, Harbin 150030, People's Republic of China.|College of Animal Science and Technology, Northeast Agricultural University, Harbin 150030, People's Republic of China.
authors.forename: Lin
authors.initials: L
authors.lastname: Guo
authors.affiliation: Key Laboratory of Chicken Genetics and Breeding, Ministry of Agriculture, Harbin 150030, People's Republic of China.|Key Laboratory of Animal Genetics, Breeding and Reproduction, Education Department of Heilongjiang Province, Harbin 150030, People's Republic of China.|College of Animal Science and Technology, Northeast Agricultural University, Harbin 150030, People's Republic of China.
authors.forename: Ning
authors.initials: N
authors.lastname: Wang
authors.affiliation: Key Laboratory of Chicken Genetics and Breeding, Ministry of Agriculture, Harbin 150030, People's Republic of China.|Key Laboratory of Animal Genetics, Breeding and Reproduction, Education Department of Heilongjiang Province, Harbin 150030, People's Republic of China.|College of Animal Science and Technology, Northeast Agricultural University, Harbin 150030, People's Republic of China.
authors.forename: Hui
authors.initials: H
authors.lastname: Shi
authors.affiliation: Key Laboratory of Chicken Genetics and Breeding, Ministry of Agriculture, Harbin 150030, People's Republic of China wyx2000@neau.edu.cn wyx405@163.com lihui@neau.edu.cn lihui645@hotmail.com.|Key Laboratory of Animal Genetics, Breeding and Reproduction, Education Department of Heilongjiang Province, Harbin 150030, People's Republic of China.|College of Animal Science and Technology, Northeast Agricultural University, Harbin 150030, People's Republic of China.
authors.forename: Yuxiang
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Key Laboratory of Chicken Genetics and Breeding, Ministry of Agriculture, Harbin 150030, People's Republic of China wyx2000@neau.edu.cn wyx405@163.com lihui@neau.edu.cn lihui645@hotmail.com.|Key Laboratory of Animal Genetics, Breeding and Reproduction, Education Department of Heilongjiang Province, Harbin 150030, People's Republic of China.|College of Animal Science and Technology, Northeast Agricultural University, Harbin 150030, People's Republic of China.
authors.forename: Hui
authors.initials: H
authors.lastname: Li
mesh_terms: D017667:Adipocytes / Q000166:cytology / Q000378:metabolism*; D000818:Animals; D030161:Avian Proteins / Q000378:metabolism; D002454:Cell Differentiation / Q000187:drug effects*; D002478:Cells, Cultured; D002645:Chickens; D066292:Lipid Droplets / Q000378:metabolism; D019301:Oleic Acid / Q000494:pharmacology*
chemical_list: D030161:Avian Proteins; D019301:Oleic Acid
keywords: adipogenesis; chicken; differentiation; fatty acid; inducer; preadipocyte