title: Genetic diversity study on 12 X-STR loci of investigator® Argus X STR kit in Bangladeshi population.
abstract: The X-chromosome short tandem repeat (STR) loci are of particular interest for solving complex kinship and paternity cases. Here, we report the genetic data from 209 unrelated Bangladeshi individuals (102 males and 107 females) that were genotyped using the 12 X-chromosomal STR markers included in the Investigator® Argus X-12 kit (Qiagen). The 12 X-STR markers are located in four linkage groups (linkage group I: DXS10135, DXS10148, and DXS8378; linkage group II: DXS7132, DXS10079, and DXS10074; linkage group III: DXS10103, HPRTB, and DXS10101; and linkage group IV: DXS10146, DXS10134, and DXS7423). Allelic frequencies of the 12 X-STR loci and haplotype frequencies of the four linkage groups were investigated. No significant difference was observed in the allele frequencies of males and females. Distributions of heterozygosity were observed from 64.5 to 92.5% among the studied 12 X STR loci. DXS10135 and DXS10101 loci were found to be most polymorphic. For all the four linkage groups, the haplotype diversity was found to be greater than 0.986. A total of 95, 73, 66, and 74 haplotypes were observed in linkage groups I, II, III, and IV, respectively. Hardy-Weinberg equilibrium tests showed no significant deviation from expected values for all 12 loci (p > 0.05). The exact test for pairwise linkage disequilibrium for the 12 loci in the male samples did not show any significant linkage disequilibrium except the DXS10103 and DXS10101 loci after the p values were corrected by Bonferroni's correction for multiple testing (p > 0.05/66). A combined power of discrimination in male and female individuals were 0.999999998159791 and 0.999999999999993, respectively. The combined mean exclusion chance were 0.999997635 in deficiency cases, 0.999999996 in normal trio cases, and 0.999999178 in duo cases. The currently investigated Bangladeshi population showed significant differences when compared with previously reported X-STR data from other 12 populations. The results of the data analysis indicated that all the loci in the Investigator® Argus X 12 kit were fairly informative and might be useful in forensic application and kinship analysis in Bangladeshi population.
authors.affiliation: National Forensic DNA Profiling Laboratory, Dhaka Medical College, Dhaka, 1000, Bangladesh.
authors.forename: Abu
authors.initials: A
authors.lastname: Sufian
authors.affiliation: Department of Biochemistry and Molecular Biology, University of Dhaka, Dhaka, 1000, Bangladesh.
authors.forename: Md Ismail
authors.initials: MI
authors.lastname: Hosen
authors.affiliation: Department of Biochemistry and Molecular Biology, University of Dhaka, Dhaka, 1000, Bangladesh.
authors.forename: Kaniz
authors.initials: K
authors.lastname: Fatema
authors.affiliation: National Forensic DNA Profiling Laboratory, Dhaka Medical College, Dhaka, 1000, Bangladesh.
authors.forename: Tania
authors.initials: T
authors.lastname: Hossain
authors.affiliation: National Forensic DNA Profiling Laboratory, Dhaka Medical College, Dhaka, 1000, Bangladesh.
authors.forename: Md Mahamud
authors.initials: MM
authors.lastname: Hasan
authors.affiliation: National Forensic DNA Profiling Laboratory, Dhaka Medical College, Dhaka, 1000, Bangladesh.
authors.forename: Ashish Kumar
authors.initials: AK
authors.lastname: Mazumder
authors.affiliation: Department of Genetic Engineering and Biotechnology, University of Dhaka, Dhaka, 1000, Bangladesh. sazaman@du.ac.bd.
authors.forename: Sharif
authors.initials: S
authors.lastname: Akhteruzzaman
mesh_terms: D001459:Bangladesh; D041321:Chromosomes, Human, X; D016172:DNA Fingerprinting; D005260:Female; D005787:Gene Frequency; D005819:Genetic Markers; D014644:Genetic Variation; D005828:Genetics, Population; D006801:Humans; D008297:Male; D018895:Microsatellite Repeats; D016133:Polymerase Chain Reaction / Q000295:instrumentation*
chemical_list: D005819:Genetic Markers
keywords: Forensic; Haplotype; Investigator® Argus X-12; Kinship analysis; Linkage groups; X-STR