title: Relative tissue distribution and excretion studies of gastrodin and parishin from powder and extract of Gastrodiae Rhizoma in rat by UHPLC-ESI-MS/MS.
abstract: New research has indicated that Gastrodiae Rhizome (GR) has potential anti-diabetic and anti-asthmatic effects in mouse models. On the basis of our previous study of the relative bioavailability of gastrodin (GAS) and parishin (PA) from extract and powder of GR, we performed further research on the tissue distribution and excretion of the two analytes. A reliable bioanalytical method for the quantification of GAS and PA in rat tissues and excretion is required. Chromatographic separation was carried out on a gradient mobile phase of acetonitrile-water with 0.1% formic acid. Calibration curves (1/x2weighted) offered satisfactory linearity (r2> 0.9835) within 100-3000 ng mL-1for GAS and (r2> 0.9862) within 10-1000 ng mL-1for PA. The relative standard deviations of the intra-day and inter-day precision were all <14.98%, whilst the relative errors of the intra-day and inter-day accuracy were all within ±14.71%. The matrix effect and recovery values were satisfactory in all of the biological matrices examination. The data of relative differences in tissue distribution and excretion of GAS and PA from powder and extract of GR indicated that higher bioavailabilities for GAS and PA were obtained when a dosage of 4 g kg-1GR powder was used.
authors.affiliation: Key Laboratory for Information System of Mountainous Areas and Protection of Ecological Environment, Guizhou Normal University, Guiyang, Guizhou, People's Republic of China.|Guizhou Engineering Laboratory for Quality Control & Evaluation Technology of Medicine, Guizhou Normal University, Guiyang, Guizhou, People's Republic of China.|The Research Center for Quality Control of Natural Medicine, Guizhou Normal University, Guiyang, Guizhou, People's Republic of China.
authors.forename: Zhengmeng
authors.initials: Z
authors.lastname: Jiang
authors.affiliation: Key Laboratory for Information System of Mountainous Areas and Protection of Ecological Environment, Guizhou Normal University, Guiyang, Guizhou, People's Republic of China.|Guizhou Engineering Laboratory for Quality Control & Evaluation Technology of Medicine, Guizhou Normal University, Guiyang, Guizhou, People's Republic of China.
authors.forename: Xiuyan
authors.initials: X
authors.lastname: Zheng
authors.affiliation: Key Laboratory for Information System of Mountainous Areas and Protection of Ecological Environment, Guizhou Normal University, Guiyang, Guizhou, People's Republic of China.|Guizhou Engineering Laboratory for Quality Control & Evaluation Technology of Medicine, Guizhou Normal University, Guiyang, Guizhou, People's Republic of China.|The Research Center for Quality Control of Natural Medicine, Guizhou Normal University, Guiyang, Guizhou, People's Republic of China.
authors.forename: Xiaojian
authors.initials: X
authors.lastname: Gong
authors.affiliation: Key Laboratory for Information System of Mountainous Areas and Protection of Ecological Environment, Guizhou Normal University, Guiyang, Guizhou, People's Republic of China.|Guizhou Engineering Laboratory for Quality Control & Evaluation Technology of Medicine, Guizhou Normal University, Guiyang, Guizhou, People's Republic of China.|The Research Center for Quality Control of Natural Medicine, Guizhou Normal University, Guiyang, Guizhou, People's Republic of China.
authors.forename: Chao
authors.initials: C
authors.lastname: Zhao
authors.affiliation: Key Laboratory for Information System of Mountainous Areas and Protection of Ecological Environment, Guizhou Normal University, Guiyang, Guizhou, People's Republic of China.|Guizhou Engineering Laboratory for Quality Control & Evaluation Technology of Medicine, Guizhou Normal University, Guiyang, Guizhou, People's Republic of China.|The Research Center for Quality Control of Natural Medicine, Guizhou Normal University, Guiyang, Guizhou, People's Republic of China.
authors.forename: Xin
authors.initials: X
authors.lastname: Zhou
authors.affiliation: Key Laboratory for Information System of Mountainous Areas and Protection of Ecological Environment, Guizhou Normal University, Guiyang, Guizhou, People's Republic of China.|Guizhou Engineering Laboratory for Quality Control & Evaluation Technology of Medicine, Guizhou Normal University, Guiyang, Guizhou, People's Republic of China.|The Research Center for Quality Control of Natural Medicine, Guizhou Normal University, Guiyang, Guizhou, People's Republic of China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: Key Laboratory for Information System of Mountainous Areas and Protection of Ecological Environment, Guizhou Normal University, Guiyang, Guizhou, People's Republic of China.|Guizhou Engineering Laboratory for Quality Control & Evaluation Technology of Medicine, Guizhou Normal University, Guiyang, Guizhou, People's Republic of China.|The Research Center for Quality Control of Natural Medicine, Guizhou Normal University, Guiyang, Guizhou, People's Republic of China.
authors.forename: Yanfang
authors.initials: Y
authors.lastname: Yan
mesh_terms: D000818:Animals; D001592:Benzyl Alcohols / Q000493:pharmacokinetics*; D001682:Biological Availability; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D031668:Gastrodia / Q000737:chemistry*; D005960:Glucosides / Q000493:pharmacokinetics*; D057230:Limit of Detection; D008297:Male; D010936:Plant Extracts / Q000493:pharmacokinetics*; D011208:Powders; D051381:Rats; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D014018:Tissue Distribution
chemical_list: D001592:Benzyl Alcohols; D005960:Glucosides; D010936:Plant Extracts; D011208:Powders; C045345:gastrodin
keywords: Gastrodiae Rhizome; UHPLC-ESI-MS/MS; bioavailability; gastrodin and parishin; tissue distribution and excretion studies