title: Increased intracellular Ca2+decreases cisplatin resistance by regulating iNOS expression in human ovarian cancer cells.
abstract: Previous studies have reported that intracellular Ca2+signals and inducible nitric oxide synthase (iNOS) are involved in cell apoptosis. However, the role of iNOS in cisplatin resistance in ovarian cancer remains unclear. Here, we demonstrate that SKOV3/DDP ovarian cancer cells were more resistant to cisplatin than were SKOV3 ovarian cancer cells. The expression of intracellular Ca2+and iNOS was more strongly induced by cisplatin in SKOV3 cells than in SKOV3/DDP cells. TAT-conjugated IP3R-derived peptide (TAT-IDPS) increased cisplatin-induced iNOS expression and apoptosis in SKOV3/DDP cells. 2-Aminoethoxydiphenyl borate (2-APB) decreased cisplatin-induced iNOS expression and apoptosis in SKOV3 cells. Thus, iNOS induction may be a valuable strategy for improving the anti-tumor efficacy of cisplatin in ovarian cancer.
authors.affiliation: Key Laboratory of Colleges and Universities of Jilin Province, Basic College of Medicine, Jilin Medical University, Jilin, Jilin, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Yu
authors.affiliation: Department of Pathophysiology, Basic College of Medicine, Jilin University, Changchun, Jilin, China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Xie
authors.affiliation: Department of Intervention, The Jilin municipal People's Hospital, Jilin, Jilin, China.
authors.forename: Weimin
authors.initials: W
authors.lastname: Liu
authors.affiliation: Key Laboratory of Colleges and Universities of Jilin Province, Basic College of Medicine, Jilin Medical University, Jilin, Jilin, China.
authors.forename: Yuting
authors.initials: Y
authors.lastname: Guo
authors.affiliation: Key Laboratory of Colleges and Universities of Jilin Province, Basic College of Medicine, Jilin Medical University, Jilin, Jilin, China.
authors.forename: Na
authors.initials: N
authors.lastname: Xu
authors.affiliation: Key Laboratory of Colleges and Universities of Jilin Province, Basic College of Medicine, Jilin Medical University, Jilin, Jilin, China.
authors.forename: Lu
authors.initials: L
authors.lastname: Xu
authors.affiliation: Key Laboratory of Colleges and Universities of Jilin Province, Basic College of Medicine, Jilin Medical University, Jilin, Jilin, China.
authors.forename: Shibing
authors.initials: S
authors.lastname: Liu
authors.affiliation: Key Laboratory of Colleges and Universities of Jilin Province, Basic College of Medicine, Jilin Medical University, Jilin, Jilin, China.
authors.forename: Songyan
authors.initials: S
authors.lastname: Li
authors.affiliation: Key Laboratory of Colleges and Universities of Jilin Province, Basic College of Medicine, Jilin Medical University, Jilin, Jilin, China. Electronic address: xuye_9707@163.com.
authors.forename: Ye
authors.initials: Y
authors.lastname: Xu
authors.affiliation: Department of Pathophysiology, Basic College of Medicine, Jilin University, Changchun, Jilin, China. Electronic address: sunlk@jlu.edu.cn.
authors.forename: Liankun
authors.initials: L
authors.lastname: Sun
mesh_terms: D000595:Amino Acid Sequence; D002118:Calcium / Q000378:metabolism*; D002470:Cell Survival / Q000187:drug effects / Q000502:physiology; D002945:Cisplatin / Q000378:metabolism* / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D019008:Drug Resistance, Neoplasm / Q000187:drug effects / Q000502:physiology*; D005260:Female; D015971:Gene Expression Regulation, Enzymologic; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007424:Intracellular Fluid / Q000187:drug effects / Q000378:metabolism*; D052247:Nitric Oxide Synthase Type II / Q000096:biosynthesis* / Q000235:genetics; D010051:Ovarian Neoplasms / Q000235:genetics / Q000378:metabolism*
chemical_list: C496319:NOS2 protein, human; D052247:Nitric Oxide Synthase Type II; D002945:Cisplatin; D002118:Calcium
keywords: Cisplatin resistance; Intracellular Ca(2+); Ovarian cancer; iNOS