title: Long noncoding RNA HIT000218960 promotes papillary thyroid cancer oncogenesis and tumor progression by upregulating the expression of high mobility group AT-hook 2 (HMGA2) gene.
abstract: Accumulating evidence suggests that long noncoding RNAs (lncRNAs) play an important role in oncogenesis and tumor progression. However, our knowledge of lncRNAs in thyroid cancer is still limited. To explore the crucial lncRNAs involved in oncogenesis of papillary thyroid cancer (PTC), we acquired data of differentially expressed lncRNAs between PTC tissues and paired adjacent noncancerous thyroid tissues through lncRNA microarray. In the microarray data, we observed that a newly identified lncRNA, HIT000218960, was significantly upregulated in PTC tissues and associated with a well-known oncogene, high mobility group AT-hook 2 (HMGA2) gene. Both in normal thyroid tissues and PTC tissues, the expression of HIT000218960 was significantly positively correlated with that of HMGA2 mRNA. Knockdown of HIT000218960 in PTC cells resulted in downregulation of HMGA2. In addition, functional assays indicated that inhibition of HIT000218960 in PTC cells suppressed cell proliferation, colony formation, migration and invasion in vitro. Increased HIT000218960 expression in PTC tissues was obviously correlated with lymph node metastasis and multifocality, as well as TNM stage. Those findings suggest that HIT000218960 might acts as a tumor promoter through regulating the expression of HMGA2.
authors.affiliation: a Department of Gastroenterological Surgery, Surgical Oncology Laboratory , Peking University People's Hospital , Xicheng District, Beijing , China.
authors.forename: Tao
authors.initials: T
authors.lastname: Li
authors.affiliation: a Department of Gastroenterological Surgery, Surgical Oncology Laboratory , Peking University People's Hospital , Xicheng District, Beijing , China.
authors.forename: Xiao-Dong
authors.initials: XD
authors.lastname: Yang
authors.affiliation: a Department of Gastroenterological Surgery, Surgical Oncology Laboratory , Peking University People's Hospital , Xicheng District, Beijing , China.
authors.forename: Chun-Xiang
authors.initials: CX
authors.lastname: Ye
authors.affiliation: a Department of Gastroenterological Surgery, Surgical Oncology Laboratory , Peking University People's Hospital , Xicheng District, Beijing , China.
authors.forename: Zhan-Long
authors.initials: ZL
authors.lastname: Shen
authors.affiliation: a Department of Gastroenterological Surgery, Surgical Oncology Laboratory , Peking University People's Hospital , Xicheng District, Beijing , China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Yang
authors.affiliation: a Department of Gastroenterological Surgery, Surgical Oncology Laboratory , Peking University People's Hospital , Xicheng District, Beijing , China.
authors.forename: Bo
authors.initials: B
authors.lastname: Wang
authors.affiliation: a Department of Gastroenterological Surgery, Surgical Oncology Laboratory , Peking University People's Hospital , Xicheng District, Beijing , China.
authors.forename: Peng
authors.initials: P
authors.lastname: Guo
authors.affiliation: a Department of Gastroenterological Surgery, Surgical Oncology Laboratory , Peking University People's Hospital , Xicheng District, Beijing , China.
authors.forename: Zhi-Dong
authors.initials: ZD
authors.lastname: Gao
authors.affiliation: a Department of Gastroenterological Surgery, Surgical Oncology Laboratory , Peking University People's Hospital , Xicheng District, Beijing , China.
authors.forename: Ying-Jiang
authors.initials: YJ
authors.lastname: Ye
authors.affiliation: a Department of Gastroenterological Surgery, Surgical Oncology Laboratory , Peking University People's Hospital , Xicheng District, Beijing , China.
authors.forename: Ke-Wei
authors.initials: KW
authors.lastname: Jiang
authors.affiliation: a Department of Gastroenterological Surgery, Surgical Oncology Laboratory , Peking University People's Hospital , Xicheng District, Beijing , China.
authors.forename: Shan
authors.initials: S
authors.lastname: Wang
mesh_terms: D000328:Adult; D063646:Carcinogenesis / Q000235:genetics* / Q000473:pathology; D002277:Carcinoma / Q000235:genetics* / Q000473:pathology; D002291:Carcinoma, Papillary; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D049109:Cell Proliferation; D018450:Disease Progression; D005260:Female; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D063990:Gene Ontology; D025743:HMGA2 Protein / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D008207:Lymphatic Metastasis / Q000235:genetics / Q000473:pathology; D008297:Male; D008875:Middle Aged; D009361:Neoplasm Invasiveness; D009367:Neoplasm Staging; D020411:Oligonucleotide Array Sequence Analysis; D062085:RNA, Long Noncoding / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D015203:Reproducibility of Results; D000077273:Thyroid Cancer, Papillary; D013964:Thyroid Neoplasms / Q000235:genetics* / Q000473:pathology; D014410:Tumor Stem Cell Assay; D015854:Up-Regulation / Q000235:genetics*
chemical_list: D025743:HMGA2 Protein; D062085:RNA, Long Noncoding; D012333:RNA, Messenger; C000620883:long noncoding RNA HIT000218960, human
keywords: HIT000218960; HMGA2; long noncoding RNA; oncogenesis; papillary thyroid cancer