title: Epigallocatechin-3-gallate inhibits adipogenesis through down-regulation of PPARγ and FAS expression mediated by PI3K-AKT signaling in 3T3-L1 cells.
abstract: Epigallocatechin-3-gallate (EGCG), a major component in green tea, functions as extensive bioactivities including anti-inflammation, anti-oxidation, and anti-cancer. However, little is known about its anti-adipogenesis and underlying mechanisms. The purport of this study sought to investigate effects of EGCG on 3T3-L1 preadipocyte differentiation and to explore its possible mechanisms. The 3T3-L1 cells were induced to differentiate under the condition of pro-adipogenic cocktail with or without indicated EGCG concentrations (10, 50, 100, 200µM) for 2, 4, 6 and 8 days, respectively. Also, another batch of 3T3-L1 cells was induced under the optimal EGCG concentration (100µM) with or without SC3036 (PI3K activator, 10µM) or SC79 (AKT activator, 0.5µM) for 8 days. Subsequently, the cell viability was examined by MTT assay and the cell morphology was visualized by Oil red O staining. Finally, the mRNA levels including peroxisome proliferator activated receptor γ (PPARγ) and fatty acid synthase (FAS) were detected by quantitative real time PCR, while the protein levels of PPARγ, FAS, phosphatidylinositol 3 kinase (PI3K), insulin receptor substrate1(IRS1), AKT, and p-AKT were measured by immunoblotting analysis. Our results showed that EGCG inhibited adipogenesis of 3T3-L1 preadipocyte in a concentration-dependent manner. Moreover, the inhibitory effects were reversed by SC3036 or SC79, suggesting that the inhibitory effects of EGCG are mediated by PI3K-AKT signaling to down-regulate PPARγ and FAS expression levels. The findings shed light on EGCG anti-adipogenic effects and its underlying mechanism and provide a novel preventive-therapeutic potential for obesity subjects as a compound from Chinese green tea.
authors.affiliation: Key Provincial Laboratory of Basic Pharmacology, Nanchang University, Nanchang 330006, Jiangxi Province, P.R. China; Department of Pharmacology, School of Pharmaceutical Science, Nanchang University, Nanchang 330006, Jiangxi Province, P.R. China.
authors.forename: Mengqing
authors.initials: M
authors.lastname: Wu
authors.affiliation: Key Provincial Laboratory of Basic Pharmacology, Nanchang University, Nanchang 330006, Jiangxi Province, P.R. China; Department of Pharmacology, School of Pharmaceutical Science, Nanchang University, Nanchang 330006, Jiangxi Province, P.R. China.
authors.forename: Dan
authors.initials: D
authors.lastname: Liu
authors.affiliation: Key Provincial Laboratory of Basic Pharmacology, Nanchang University, Nanchang 330006, Jiangxi Province, P.R. China; Department of Pharmacology, School of Pharmaceutical Science, Nanchang University, Nanchang 330006, Jiangxi Province, P.R. China.
authors.forename: Rong
authors.initials: R
authors.lastname: Zeng
authors.affiliation: Key Provincial Laboratory of Basic Pharmacology, Nanchang University, Nanchang 330006, Jiangxi Province, P.R. China; Department of Pharmacology, School of Pharmaceutical Science, Nanchang University, Nanchang 330006, Jiangxi Province, P.R. China.
authors.forename: Tao
authors.initials: T
authors.lastname: Xian
authors.affiliation: Key Provincial Laboratory of Basic Pharmacology, Nanchang University, Nanchang 330006, Jiangxi Province, P.R. China; Department of Pharmacology, School of Pharmaceutical Science, Nanchang University, Nanchang 330006, Jiangxi Province, P.R. China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Lu
authors.affiliation: Key Provincial Laboratory of Basic Pharmacology, Nanchang University, Nanchang 330006, Jiangxi Province, P.R. China; Department of Pharmacology, School of Pharmaceutical Science, Nanchang University, Nanchang 330006, Jiangxi Province, P.R. China.
authors.forename: Guohua
authors.initials: G
authors.lastname: Zeng
authors.affiliation: Jiangxi Medical School, Nanchang University, Nanchang 330006, Jiangxi Province, P.R. China.
authors.forename: Zhangzetian
authors.initials: Z
authors.lastname: Sun
authors.affiliation: Jiangxi Medical School, Nanchang University, Nanchang 330006, Jiangxi Province, P.R. China.
authors.forename: Bowei
authors.initials: B
authors.lastname: Huang
authors.affiliation: Key Provincial Laboratory of Basic Pharmacology, Nanchang University, Nanchang 330006, Jiangxi Province, P.R. China; Department of Pharmacology, School of Pharmaceutical Science, Nanchang University, Nanchang 330006, Jiangxi Province, P.R. China. Electronic address: qrhuang@ncu.edu.cn.
authors.forename: Qiren
authors.initials: Q
authors.lastname: Huang
mesh_terms: D041721:3T3-L1 Cells; D017667:Adipocytes / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D050156:Adipogenesis / Q000187:drug effects*; D000818:Animals; D002392:Catechin / Q000031:analogs & derivatives* / Q000494:pharmacology; D002470:Cell Survival / Q000187:drug effects; D015536:Down-Regulation / Q000187:drug effects*; D064429:Fatty Acid Synthases / Q000235:genetics*; D055504:Insulin Receptor Substrate Proteins / Q000378:metabolism; D051379:Mice; D047495:PPAR gamma / Q000235:genetics*; D019869:Phosphatidylinositol 3-Kinases / Q000378:metabolism*; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism*; D015398:Signal Transduction / Q000187:drug effects
chemical_list: D055504:Insulin Receptor Substrate Proteins; C526295:Irs1 protein, mouse; D047495:PPAR gamma; D002392:Catechin; C045651:epigallocatechin gallate; D064429:Fatty Acid Synthases; D019869:Phosphatidylinositol 3-Kinases; D051057:Proto-Oncogene Proteins c-akt
keywords: Adipocyte; Adipogenesis; Epigallocatechin-3-gallate; Peroxisome proliferator-activated receptor gamma; Phosphatidylinositol 3-kinase