title: Chemoenzymatic synthesis of para-nitrophenol (pNP)-tagged α2-8-sialosides and high-throughput substrate specificity studies of α2-8-sialidases.
abstract: para-Nitrophenol (pNP)-tagged α2-8-linked sialosides containing different sialic acid forms were chemoenzymatically synthesized using an efficient one-pot three-enzyme α2-8-sialylation system. The resulting compounds allowed high-throughput substrate specificity studies of the α2-8-sialidase activity of a recombinant human cytosolic sialidase hNEU2 and various bacterial sialidases. The sialoside substrate profiles obtained can be used to guide the selection of suitable sialidases for sialylglycan analysis and for cell and tissue surface glycan modification. They can also be used to guide sialidase inhibitor design.
authors.affiliation: Department of Chemistry, University of California, One Shields Avenue, Davis, CA 95616, USA. xiichen@ucdavis.edu.
authors.forename: Nova
authors.initials: N
authors.lastname: Tasnima
authors.affiliation: Department of Chemistry, University of California, One Shields Avenue, Davis, CA 95616, USA. xiichen@ucdavis.edu.
authors.forename: Hai
authors.initials: H
authors.lastname: Yu
authors.affiliation: Department of Chemistry, University of California, One Shields Avenue, Davis, CA 95616, USA. xiichen@ucdavis.edu.
authors.forename: Yanhong
authors.initials: Y
authors.lastname: Li
authors.affiliation: Department of Chemistry, University of California, One Shields Avenue, Davis, CA 95616, USA. xiichen@ucdavis.edu.
authors.forename: Abhishek
authors.initials: A
authors.lastname: Santra
authors.affiliation: Department of Chemistry, University of California, One Shields Avenue, Davis, CA 95616, USA. xiichen@ucdavis.edu.
authors.forename: Xi
authors.initials: X
authors.lastname: Chen
mesh_terms: D001419:Bacteria / Q000201:enzymology*; D057166:High-Throughput Screening Assays / Q000379:methods; D006801:Humans; D009439:Neuraminidase / Q000378:metabolism*; D009596:Nitrophenols / Q000138:chemical synthesis* / Q000737:chemistry / Q000378:metabolism*; D011994:Recombinant Proteins / Q000378:metabolism; D012794:Sialic Acids / Q000138:chemical synthesis* / Q000737:chemistry / Q000378:metabolism*; D013379:Substrate Specificity
chemical_list: D009596:Nitrophenols; D011994:Recombinant Proteins; D012794:Sialic Acids; C403285:NEU2 protein, human; D009439:Neuraminidase; C024836:4-nitrophenol