title: Multiplex Detection of Fusarium Species.
abstract: Multiplex PCR is a powerful method to detect, identify, and quantify the mycotoxigenic fungus by targeting the amplification of genes associated with mycotoxin production and detection, identification, and quantification of Fusarium species. As compared with uniplex PCR, it has several advantages such as low cost, shortened time, and simultaneous amplification of more than two genes (in only one reaction tube). Here, we describe multiplex PCR-based detection and identification of trichothecene-, zearalenone-, fumonisin-, and enniatin-producing Fusarium species, the use of multiplex PCR in multiplex genotype assay and the use of multiplex TaqMan real-time qPCR.
authors.affiliation: Molecular Plant Biology, Department of Biochemistry, University of Turku, FI-20520, Turku, Finland. tymat@utu.fi.
authors.forename: Tapani
authors.initials: T
authors.lastname: Yli-Mattila
authors.affiliation: DOS in Biotechnology, University of Mysore, Manasagangotri, Mysuru, India.
authors.forename: Siddaiah Chandra
authors.initials: SC
authors.lastname: Nayaka
authors.affiliation: Microbiology Division, DRDO-BU-Centre for Life sciences, Bharathiar University Campus, Coimbatore, 641046, TamilNadu, India.
authors.forename: Mudili
authors.initials: M
authors.lastname: Venkataramana
authors.affiliation: Department of Molecular Biology and Genetics, Faculty of Arts and Sciences, Istanbul Yeni Yuzyil University, 34010, Istanbul, Turkey.
authors.forename: Emre
authors.initials: E
authors.lastname: Yörük
mesh_terms: D004271:DNA, Fungal; D005670:Fusarium / Q000145:classification* / Q000235:genetics* / Q000378:metabolism; D005800:Genes, Fungal; D005838:Genotype; D058885:Multilocus Sequence Typing; D060885:Multiplex Polymerase Chain Reaction; D009183:Mycotoxins / Q000096:biosynthesis; D060888:Real-Time Polymerase Chain Reaction
chemical_list: D004271:DNA, Fungal; D009183:Mycotoxins
keywords: Enniatins; F. avenaceum; F. culmorum; F. tricinctum; F. verticillioides; Fumonisins; Fusarium graminearum; Moniliformin; Trichothecenes; Zearalenone