title: A methodology for finding the optimal iteration number of the SIRT algorithm for quantitative Electron Tomography.
abstract: The SIRT (Simultaneous Iterative Reconstruction Technique) algorithm is commonly used in Electron Tomography to calculate the original volume of the sample from noisy images, but the results provided by this iterative procedure are strongly dependent on the specific implementation of the algorithm, as well as on the number of iterations employed for the reconstruction. In this work, a methodology for selecting the iteration number of the SIRT reconstruction that provides the most accurate segmentation is proposed. The methodology is based on the statistical analysis of the intensity profiles at the edge of the objects in the reconstructed volume. A phantom which resembles a a carbon black aggregate has been created to validate the methodology and the SIRT implementations of two free software packages (TOMOJ and TOMO3D) have been used.
authors.affiliation: eMERG, Fisika Aplikatua I Saila, Faculty of Engineering, University of the Basque Country, UPV/EHU, Rafael Moreno "Pitxitxi" Pasealekua 3, 48013 Bilbao, Spain. Electronic address: ana.okariz@ehu.es.
authors.forename: Ana
authors.initials: A
authors.lastname: Okariz
authors.affiliation: eMERG, Departamento de Ingeniería Minera y Metalúrgica y Ciencia de los Materiales, Faculty of Engineering, University of the Basque Country, UPV/EHU, Rafael Moreno "Pitxitxi" Pasealekua 3, 48013 Bilbao, Spain.
authors.forename: Teresa
authors.initials: T
authors.lastname: Guraya
authors.affiliation: eMERG, Departamento de Expresión Gráfica y Proyectos de Ingeniería, Faculty of Engineering, University of the Basque Country, UPV/EHU, Rafael Moreno "Pitxitxi" Pasealekua 3, 48013 Bilbao, Spain.
authors.forename: Maider
authors.initials: M
authors.lastname: Iturrondobeitia
authors.affiliation: eMERG, Fisika Aplikatua I Saila, Faculty of Engineering,University of the Basque Country, UPV/EHU, Rafael Moreno "Pitxitxi" Pasealekua 2, 48013 Bilbao, Spain.
authors.forename: Julen
authors.initials: J
authors.lastname: Ibarretxe
mesh_terms: D000465:Algorithms; D055032:Electron Microscope Tomography / Q000379:methods*; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D012984:Software
keywords: Electron tomography; SIRT; TEM; resolution