title: Design, synthesis, MTT assay, DNA interaction studies of platinum(II) complexes.
abstract: The square planar Pt(II) complexes of the type [Pt(Ln)(Cl2)] (where Ln= L1-3= thiophene-2-carboxamide derivatives and L4-6= thiophene-2-carbothioamide derivatives) have been synthesized and characterized by physicochemical and various spectroscopic studies. MIC method was employed to inference the antibacterial potency of complexes in reference to free ligands and metal salt. Characteristic binding constant (Kb) and binding mode of complexes with calf thymus DNA (CT-DNA) were determined using absorption titration (0.76-1.61 × 105M-1), hydrodynamic chain length assay and fluorescence quenching analysis, deducing the partial intercalative mode of binding. Molecular docking calculation displayed free energy of binding in the range of -260.06 to -219.63 kJmol-1. The nuclease profile of complexes towards pUC19 DNA shows that the complexes cleave DNA more efficiently compared to their respective metal salt. Cytotoxicity profile of the complexes on the brine shrimp shows that all the complex exhibit noteworthy cytotoxic activity with LC50values ranging from 7.87 to 15.94 μg/mL. The complexes have been evaluated for cell proliferation potential in human colon carcinoma cells (HCT 116) and IC50value of complexes by MTT assay (IC50= 125-1000 μg/mL).
authors.affiliation: a Department of Chemistry , Sardar Patel University , Vallabh Vidyanagar 388 120 , Gujarat , India.
authors.forename: Miral V
authors.initials: MV
authors.lastname: Lunagariya
authors.affiliation: a Department of Chemistry , Sardar Patel University , Vallabh Vidyanagar 388 120 , Gujarat , India.
authors.forename: Khyati P
authors.initials: KP
authors.lastname: Thakor
authors.affiliation: b Department of Cell biology , Indian Institute of Advanced Research , Gandhinagar , Gujarat , India.
authors.forename: Bhargav N
authors.initials: BN
authors.lastname: Waghela
authors.affiliation: b Department of Cell biology , Indian Institute of Advanced Research , Gandhinagar , Gujarat , India.
authors.forename: Foram U
authors.initials: FU
authors.lastname: Vaidya
authors.affiliation: b Department of Cell biology , Indian Institute of Advanced Research , Gandhinagar , Gujarat , India.
authors.forename: Chadramani
authors.initials: C
authors.lastname: Pathak
authors.affiliation: a Department of Chemistry , Sardar Patel University , Vallabh Vidyanagar 388 120 , Gujarat , India.
authors.forename: Mohan N
authors.initials: MN
authors.lastname: Patel
mesh_terms: D000818:Animals; D001156:Artemia / Q000187:drug effects / Q000254:growth & development; D001419:Bacteria / Q000187:drug effects / Q000254:growth & development; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D056831:Coordination Complexes / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology; D004247:DNA / Q000737:chemistry* / Q000235:genetics; D015195:Drug Design; D045325:HCT116 Cells; D006801:Humans; D008826:Microbial Sensitivity Tests; D062105:Molecular Docking Simulation; D010984:Platinum / Q000737:chemistry*; D013876:Thiophenes / Q000737:chemistry
chemical_list: D056831:Coordination Complexes; D013876:Thiophenes; D010984:Platinum; D004247:DNA
keywords: DNA interaction study; antibacterial study; brine shrimp cytotoxicity assay; cell proliferation assay; platinum(II) complexes