title: Characterization, Quantification, and Visualization of Neutrophil Extracellular Traps.
abstract: Following the discovery of neutrophil extracellular traps (NETs) in 2004 by Brinkmann and colleagues, there has been extensive research into the role of NETs in a number of inflammatory diseases, including periodontitis. This chapter describes the current methods for the isolation of peripheral blood neutrophils for subsequent NET experiments, including approaches to quantify and visualize NET production, the ability of NETs to entrap and kill bacteria, and the removal of NETs by nuclease-containing plasma.
authors.affiliation: Institute of Clinical Sciences, College of Medical and Dental Sciences, The School of Dentistry, University of Birmingham, 5 Mill Pool Way, Edgbaston, Birmingham, B5 7EG, UK.
authors.forename: Phillipa C
authors.initials: PC
authors.lastname: White
authors.affiliation: Institute of Clinical Sciences, College of Medical and Dental Sciences, The School of Dentistry, University of Birmingham, 5 Mill Pool Way, Edgbaston, Birmingham, B5 7EG, UK.
authors.forename: Ilaria J
authors.initials: IJ
authors.lastname: Chicca
authors.affiliation: Institute of Clinical Sciences, College of Medical and Dental Sciences, The School of Dentistry, University of Birmingham, 5 Mill Pool Way, Edgbaston, Birmingham, B5 7EG, UK.
authors.forename: Martin R
authors.initials: MR
authors.lastname: Ling
authors.affiliation: Institute of Clinical Sciences, College of Medical and Dental Sciences, The School of Dentistry, University of Birmingham, 5 Mill Pool Way, Edgbaston, Birmingham, B5 7EG, UK.
authors.forename: Helen J
authors.initials: HJ
authors.lastname: Wright
authors.affiliation: Institute of Clinical Sciences, College of Medical and Dental Sciences, The School of Dentistry, University of Birmingham, 5 Mill Pool Way, Edgbaston, Birmingham, B5 7EG, UK.
authors.forename: Paul R
authors.initials: PR
authors.lastname: Cooper
authors.affiliation: Institute of Clinical Sciences, College of Medical and Dental Sciences, The School of Dentistry, University of Birmingham, 5 Mill Pool Way, Edgbaston, Birmingham, B5 7EG, UK.
authors.forename: Mike R
authors.initials: MR
authors.lastname: Milward
authors.affiliation: Institute of Clinical Sciences, College of Medical and Dental Sciences, The School of Dentistry, University of Birmingham, 5 Mill Pool Way, Edgbaston, Birmingham, B5 7EG, UK. i.l.c.chapple@bham.ac.uk.
authors.forename: Iain L C
authors.initials: IL
authors.lastname: Chapple
mesh_terms: D015415:Biomarkers; D056649:Cathepsin G / Q000378:metabolism; D003602:Cytotoxicity, Immunologic; D065206:Extracellular Traps / Q000276:immunology / Q000378:metabolism* / Q000382:microbiology; D006801:Humans; D019272:Leukocyte Elastase / Q000378:metabolism; D050296:Microbial Viability / Q000276:immunology; D008856:Microscopy, Fluorescence; D018375:Neutrophil Activation / Q000276:immunology; D009504:Neutrophils / Q000276:immunology / Q000378:metabolism* / Q000382:microbiology / Q000648:ultrastructure; D017382:Reactive Oxygen Species / Q000378:metabolism
chemical_list: D015415:Biomarkers; D017382:Reactive Oxygen Species; D056649:Cathepsin G; D019272:Leukocyte Elastase
keywords: Cathepsin G; Chemiluminescence; DNA; Elastase; Fluorescence microscopy; Immunostaining; Myeloperoxidase; Neutrophil extracellular traps; Reactive oxygen species; SEM