title: Analysis of Bacterial Surface Interactions with Mass Spectrometry-Based Proteomics.
abstract: Host-pathogen protein-protein interaction networks are highly complex and dynamic. In this experimental protocol we describe a method to isolate host proteins attached to the bacterial surface followed by quantitative mass spectrometry based proteomics analysis. This technique provides an overview of the host-pathogen interaction network, which can be used to guide directed perturbations of the system, and to select target of specific interest for further studies.
authors.affiliation: Division of Infection Medicine, Department of Clinical Sciences, Lund University, Biomedical Center D13, Lund 22184, Sweden.
authors.forename: Christofer
authors.initials: C
authors.lastname: Karlsson
authors.affiliation: Division of Infection Medicine, Department of Clinical Sciences, Lund University, Biomedical Center D13, Lund 22184, Sweden.|Department of Immunotechnology, Lund University, Medicon Village 406, Lund 22381, Sweden.
authors.forename: Johan
authors.initials: J
authors.lastname: Teleman
authors.affiliation: Division of Infection Medicine, Department of Clinical Sciences, Lund University, Biomedical Center D13, Lund 22184, Sweden. johan.malmstrom@med.lu.se.
authors.forename: Johan
authors.initials: J
authors.lastname: Malmström
mesh_terms: D000327:Adsorption; D001419:Bacteria / Q000378:metabolism*; D001798:Blood Proteins / Q000302:isolation & purification / Q000378:metabolism; D054884:Host-Pathogen Interactions; D013058:Mass Spectrometry / Q000379:methods; D011485:Protein Binding; D025941:Protein Interaction Mapping / Q000379:methods*; D040901:Proteomics / Q000379:methods; D052616:Solid Phase Extraction; D014357:Trypsin / Q000378:metabolism
chemical_list: D001798:Blood Proteins; D014357:Trypsin
keywords: Bacteria; Bioinformatics; Mass spectrometry; Peptide solid phase extraction; Proteomics; Surface absorption; Trypsin digestion