title: Osteoblast adhesion, migration, and proliferation variations on chemically patterned nanocrystalline diamond films evaluated by live-cell imaging.
abstract: Cell fate modulation by adapting the surface of a biocompatible material is nowadays a challenge in implantology, tissue engineering as well as in construction of biosensors. Nanocrystalline diamond (NCD) thin films are considered promising in these fields due to their extraordinary physical and chemical properties and diverse ways in which they can be modified structurally and chemically. The initial cell distribution, the rate of cell adhesion, distance of cell migration and also the cell proliferation are influenced by the NCD surface termination. Here, we use real-time live-cell imaging to investigate the above-mentioned processes on oxidized NCD (NCD-O) and hydrogenated NCD (NCD-H) to elucidate cell preference to the NCD-O especially on surfaces with microscopic surface termination patterns. Cells adhere more slowly and migrate farther on NCD-H than on NCD-O. Cells seeded with a fetal bovine serum (FBS) supplement in the medium move across the surface prior to adhesion. In the absence of FBS, the cells adhere immediately, but still exhibit different migration and proliferation on NCD-O/H regions. We discuss the impact of these effects on the formation of cell arrays on micropatterned NCD. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 1469-1478, 2017.
authors.affiliation: Institute of Inherited Metabolic Disorders, Laboratory of Interaction of Cells with Nanomaterials, 1st Faculty of Medicine, Charles University in Prague, Ke Karlovu 2, 12853 Prague 2, Czech Republic.|Institute of Physiology, Department of Biomaterials and Tissue Engineering, Czech Academy of Sciences, v. v. i, Videnska 1083, 142 20, Prague 4, Czech Republic.
authors.forename: Antonin
authors.initials: A
authors.lastname: Broz
authors.affiliation: Institute of Physics, Department of Optical Materials, Czech Academy of Sciences, v. v. i, Cukrovarnicka 10, 162 53 Prague 6, Czech Republic.
authors.forename: Egor
authors.initials: E
authors.lastname: Ukraintsev
authors.affiliation: Institute of Physics, Department of Optical Materials, Czech Academy of Sciences, v. v. i, Cukrovarnicka 10, 162 53 Prague 6, Czech Republic.
authors.forename: Alexander
authors.initials: A
authors.lastname: Kromka
authors.affiliation: Institute of Physics, Department of Optical Materials, Czech Academy of Sciences, v. v. i, Cukrovarnicka 10, 162 53 Prague 6, Czech Republic.|Faculty of Electrical Engineering, Department of Physics, Czech Technical University, Technicka 2, 166 27 Prague 6, Czech Republic.
authors.forename: Bohuslav
authors.initials: B
authors.lastname: Rezek
authors.affiliation: Institute of Inherited Metabolic Disorders, Laboratory of Interaction of Cells with Nanomaterials, 1st Faculty of Medicine, Charles University in Prague, Ke Karlovu 2, 12853 Prague 2, Czech Republic.|Biomedical Centre, Laboratory of Cell-Biomaterial Interactions, Faculty of Medicine in Pilsen, Charles University, alej Svobody 76, 323 00 Pilsen, Czech Republic.
authors.forename: Marie
authors.identifier: 0000-0002-3886-2218
authors.initials: M
authors.lastname: Hubalek Kalbacova
mesh_terms: D002448:Cell Adhesion; D002460:Cell Line; D002465:Cell Movement; D049109:Cell Proliferation; D006801:Humans; D008567:Membranes, Artificial; D058612:Nanodiamonds / Q000737:chemistry*; D010006:Osteoblasts / Q000166:cytology / Q000378:metabolism
chemical_list: D008567:Membranes, Artificial; D058612:Nanodiamonds
keywords: adhesion; live-cell imaging; migration; osteoblasts; patterned surface; proliferation