title: Inter-laboratory study to characterize the detection of serum antibodies against porcine epidemic diarrhoea virus.
abstract: Porcine epidemic diarrhea virus (PEDV) has caused extensive economic losses to pig producers in many countries. It was recently introduced, for the first time, into North America and outbreaks have occurred again in multiple countries within Europe as well. To assess the properties of various diagnostic assays for the detection of PEDV infection, multiple panels of porcine sera have been shared and tested for the presence of antibodies against PEDV in an inter-laboratory ring trial. Different laboratories have used a variety of "in house" ELISAs and also one commercial assay. The sensitivity and specificity of each assay has been estimated using a Bayesian analysis applied to the ring trial results obtained with the different assays in the absence of a gold standard. Although different characteristics were found, it can be concluded that each of the assays used can detect infection of pigs at a herd level by either the early European strains of PEDV or the recently circulating strains (INDEL and non-INDEL). However, not all the assays seem suitable for demonstrating freedom from disease in a country. The results from individual animals, especially when the infection has occurred within an experimental situation, show more variation.
authors.affiliation: National Veterinary Institute, Technical University of Denmark, Lindholm, Kalvehave 4771, Denmark. Electronic address: bstr@vet.dtu.dk.
authors.forename: Bertel
authors.initials: B
authors.lastname: Strandbygaard
authors.affiliation: IZSLER - Virology Unit, Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia Romagna "Bruno Ubertini" Via Bianchi 7/9, 25124 Brescia, Italy. Electronic address: antonio.lavazza@izsler.it.
authors.forename: Antonio
authors.initials: A
authors.lastname: Lavazza
authors.affiliation: IZSLER - Virology Unit, Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia Romagna "Bruno Ubertini" Via Bianchi 7/9, 25124 Brescia, Italy. Electronic address: davide.lelli@izsler.it.
authors.forename: Davide
authors.initials: D
authors.lastname: Lelli
authors.affiliation: ANSES - Laboratory of Ploufragan-Plouzané - BP 53, 22440 Ploufragan, France; Université Bretagne Loire, Rennes, France. Electronic address: Yannick.BLANCHARD@anses.fr.
authors.forename: Yannick
authors.initials: Y
authors.lastname: Blanchard
authors.affiliation: ANSES - Laboratory of Ploufragan-Plouzané - BP 53, 22440 Ploufragan, France; Université Bretagne Loire, Rennes, France. Electronic address: Beatrice.GRASLAND@anses.fr.
authors.forename: Béatrice
authors.initials: B
authors.lastname: Grasland
authors.affiliation: Université Paris-Est, Ecole Nationale Vétérinaire d'Alfort, UMR 1161 Virology, Maisons-Alfort, France; INRA, UMR 1161 Virology, Maisons-Alfort, France; ANSES - Laboratory of Animal Health of Maisons-Alfort - UMR 1161 Virology, 23 avenue du Général de Gaulle, France. Electronic address: slepoder@vet-alfort.fr.
authors.forename: Sophie Le
authors.initials: SL
authors.lastname: Poder
authors.affiliation: ANSES - Laboratory of Ploufragan-Plouzané - BP 53, 22440 Ploufragan, France; Université Bretagne Loire, Rennes, France. Electronic address: Nicolas.ROSE@anses.fr.
authors.forename: Nicolas
authors.initials: N
authors.lastname: Rose
authors.affiliation: APHA, Dept of Virology, Weybridge, Addlestone, Surrey. KT15 3NB, UK. Electronic address: Falko.Steinbach@ahvla.gsi.gov.uk.
authors.forename: Falko
authors.initials: F
authors.lastname: Steinbach
authors.affiliation: Wageningen University and Research Centre, Edelhertweg 15, 8219PH, Lelystad, The Netherlands. Electronic address: wim.vanderpoel@wur.nl.
authors.forename: Wim H M
authors.initials: WHM
authors.lastname: van der Poel
authors.affiliation: The National Veterinary Institute (SVA), 75189 Uppsala, Sweden. Electronic address: frederik.widen@sva.se.
authors.forename: Frederik
authors.initials: F
authors.lastname: Widén
authors.affiliation: National Veterinary Institute, Technical University of Denmark, Lindholm, Kalvehave 4771, Denmark. Electronic address: grbe@vet.dtu.dk.
authors.forename: Graham J
authors.initials: GJ
authors.lastname: Belsham
authors.affiliation: National Veterinary Institute, Technical University of Denmark, Lindholm, Kalvehave 4771, Denmark. Electronic address: aneb@vet.dtu.dk.
authors.forename: Anette
authors.initials: A
authors.lastname: Bøtner
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000662:veterinary*; D053485:Porcine epidemic diarrhea virus / Q000276:immunology*; D012680:Sensitivity and Specificity; D012698:Serologic Tests / Q000662:veterinary*; D013552:Swine
chemical_list: D000914:Antibodies, Viral
keywords: ELISA; PEDV; Porcine coronavirus; Ring trial; Serology