title: Influence of Biodentine® - A Dentine Substitute - On Collagen Type I Synthesis in Pulp Fibroblasts In Vitro.
abstract: Preserving a patient's own teeth-even in a difficult situation-is nowadays preferable to surgical intervention and therefore promotes development of suitable dental repair materials. Biodentine®, a mineral trioxide aggregate substitute, has been used to replace dentine in a bioactive and biocompatible manner in both the dental crown and the root. The aim of our study was to evaluate the influence of Biodentine® on pulp fibroblasts in vitro. For this study, one to five Biodentine® discs with a diameter of 5.1mm were incubated in DMEM. To obtain Biodentine® suspensions the media were collected and replaced with fresh medium every 24h for 4 days. Primary pulp cells were isolated from freshly extracted wisdom teeth of 20-23 year old patients and incubated with the Biodentine® suspensions. Proliferation, cell morphology, cell integrity and cell viability were monitored. To evaluate the effect of Biodentine® on collagen type I synthesis, the secretion of the N-terminal domain of pro-collagen type I (P1NP) and the release of transforming growth factor-β1 (TGF-β1) were quantified. None of the Biodentine® suspensions tested influenced cell morphology, proliferation or cell integrity. The cell viability varied slightly depending on the suspension used. However, the concentrations of P1NP of all pulp fibroblast cultures treated for 24h with the moderate to high Biodentine® concentration containing suspensions of day 1 were reduced to 5% of the control. Furthermore, a significant TGF-β1 reduction was observed after treatment with these suspensions. It could be shown that Biodentine® is biocompatible. However, dissolved particles of the moderate to high concentrated Biodentine® suspensions 24h after mixing induce a significant reduction of TGF-β1 release and reduce the secretion of collagen type I of primary pulp fibroblasts.
authors.affiliation: Department of Dermatology, Venereology and Allergology, Johann Wolfgang Goethe-University, Frankfurt/Main, Germany.|Department of Operative Dentistry, Center for Dentistry and Oral Medicine (Carolinum), Johann Wolfgang Goethe-University, Frankfurt/Main, Germany.
authors.forename: Frangis
authors.initials: F
authors.lastname: Nikfarjam
authors.affiliation: Department of Dermatology, Venereology and Allergology, Johann Wolfgang Goethe-University, Frankfurt/Main, Germany.|Department of Operative Dentistry, Center for Dentistry and Oral Medicine (Carolinum), Johann Wolfgang Goethe-University, Frankfurt/Main, Germany.
authors.forename: Kim
authors.initials: K
authors.lastname: Beyer
authors.affiliation: Department of Dermatology, Venereology and Allergology, Johann Wolfgang Goethe-University, Frankfurt/Main, Germany.
authors.forename: Anke
authors.initials: A
authors.lastname: König
authors.affiliation: Department of Dermatology, Venereology and Allergology, Johann Wolfgang Goethe-University, Frankfurt/Main, Germany.
authors.forename: Matthias
authors.initials: M
authors.lastname: Hofmann
authors.affiliation: Department of Dermatology, Venereology and Allergology, Johann Wolfgang Goethe-University, Frankfurt/Main, Germany.
authors.forename: Manuel
authors.initials: M
authors.lastname: Butting
authors.affiliation: Department of Dermatology, Venereology and Allergology, Johann Wolfgang Goethe-University, Frankfurt/Main, Germany.
authors.forename: Eva
authors.initials: E
authors.lastname: Valesky
authors.affiliation: Department of Dermatology, Venereology and Allergology, Johann Wolfgang Goethe-University, Frankfurt/Main, Germany.
authors.forename: Stefan
authors.initials: S
authors.lastname: Kippenberger
authors.affiliation: Department of Dermatology, Venereology and Allergology, Johann Wolfgang Goethe-University, Frankfurt/Main, Germany.
authors.forename: Roland
authors.initials: R
authors.lastname: Kaufmann
authors.affiliation: Department of Operative Dentistry, Center for Dentistry and Oral Medicine (Carolinum), Johann Wolfgang Goethe-University, Frankfurt/Main, Germany.
authors.forename: Detlef
authors.initials: D
authors.lastname: Heidemann
authors.affiliation: Department of Dermatology, Venereology and Allergology, Johann Wolfgang Goethe-University, Frankfurt/Main, Germany.
authors.forename: August
authors.initials: A
authors.lastname: Bernd
authors.affiliation: Department of Dermatology, Venereology and Allergology, Johann Wolfgang Goethe-University, Frankfurt/Main, Germany.
authors.forename: Nadja Nicole
authors.identifier: http://orcid.org/0000-0003-2438-2747
authors.initials: NN
authors.lastname: Zöller
mesh_terms: D000328:Adult; D017610:Calcium Compounds / Q000494:pharmacology*; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D024042:Collagen Type I / Q000378:metabolism*; D003782:Dental Pulp / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D006801:Humans; D057872:Pulp Capping and Pulpectomy Agents / Q000494:pharmacology*; D017640:Silicates / Q000494:pharmacology*; D053773:Transforming Growth Factor beta1 / Q000378:metabolism; D055815:Young Adult
chemical_list: D017610:Calcium Compounds; D024042:Collagen Type I; D057872:Pulp Capping and Pulpectomy Agents; D017640:Silicates; D053773:Transforming Growth Factor beta1; C506393:tricalcium silicate