title: Identifying Bacterial Immune Evasion Proteins Using Phage Display.
abstract: Methods aimed at identification of immune evasion proteins are mainly rely on in silico prediction of sequence, structural homology to known evasion proteins or use a proteomics driven approach. Although proven successful these methods are limited by a low efficiency and or lack of functional identification. Here we describe a high-throughput genomic strategy to functionally identify bacterial immune evasion proteins using phage display technology. Genomic bacterial DNA is randomly fragmented and ligated into a phage display vector that is used to create a phage display library expressing bacterial secreted and membrane bound proteins. This library is used to select displayed bacterial secretome proteins that interact with host immune components.
authors.affiliation: Department of Medical Microbiology, University Medical Center Utrecht, Heidelberglaan 100, 3484 CX, Utrecht, The Netherlands.
authors.forename: Cindy
authors.initials: C
authors.lastname: Fevre
authors.affiliation: Department of Medical Microbiology, University Medical Center Utrecht, Heidelberglaan 100, 3484 CX, Utrecht, The Netherlands.
authors.forename: Lisette
authors.initials: L
authors.lastname: Scheepmaker
authors.affiliation: Department of Medical Microbiology, University Medical Center Utrecht, Heidelberglaan 100, 3484 CX, Utrecht, The Netherlands. P.J.A.Haas@umcutrecht.nl.
authors.forename: Pieter-Jan
authors.initials: PJ
authors.lastname: Haas
mesh_terms: D001419:Bacteria / Q000276:immunology* / Q000378:metabolism* / Q000472:pathogenicity; D001426:Bacterial Proteins / Q000276:immunology* / Q000378:metabolism*; D061505:Cell Surface Display Techniques; D015698:Genomic Library; D057166:High-Throughput Screening Assays; D057131:Immune Evasion; D019151:Peptide Library; D020543:Proteome; D040901:Proteomics / Q000379:methods
chemical_list: D001426:Bacterial Proteins; D019151:Peptide Library; D020543:Proteome
keywords: Functional identification; High-throughput; Immune evasion; Phage display; Secretome