title: Extract of Syringa oblata: A new biocontrol agent against tobacco bacterial wilt caused by Ralstonia solanacearum.
abstract: Ralstonia solanacearum causes serious wilt disease in tobacco. To effectively control this disease, the antibacterial activity of 95% ethanol extracts from the flower buds of Syringa oblata was examined. Based on GC-MS analysis and an inhibition experiment against R. solanacearum, the main antibacterial component is eugenol. We further determined the effect of eugenol on the physiology, biochemistry, and cellular morphology of R. solanacearum. The results showed that eugenol can destroy wilt bacteria, leading to the disappearance of flagella, the leakage of contents, and the appearance of a cavity. SDS-PAGE showed that eugenol decreased protein content in R. solanacearum, reduced medium carbohydrate utilization, and inhibited CAT and SDH activity. The above results showed that eugenol had a significant inhibitory effect on R. solanacearum and this component has the potential to prevent tobacco bacterial wilt.
authors.affiliation: Pest Integrated Management Key Laboratory of China Tobacco, Tobacco Research Institute of Chinese Academy of Agricultural Sciences, Qingdao, China; Tobacco Research Institute of Nanping, Nangping, China.
authors.forename: Wanming
authors.initials: W
authors.lastname: Bai
authors.affiliation: Pest Integrated Management Key Laboratory of China Tobacco, Tobacco Research Institute of Chinese Academy of Agricultural Sciences, Qingdao, China.
authors.forename: Fanyu
authors.initials: F
authors.lastname: Kong
authors.affiliation: Tobacco Research Institute of Nanping, Nangping, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Lin
authors.affiliation: Pest Integrated Management Key Laboratory of China Tobacco, Tobacco Research Institute of Chinese Academy of Agricultural Sciences, Qingdao, China. Electronic address: zhangchengsheng@caas.cn.
authors.forename: Chengsheng
authors.initials: C
authors.lastname: Zhang
mesh_terms: D000900:Anti-Bacterial Agents / Q000302:isolation & purification / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000378:metabolism; D002374:Catalase / Q000378:metabolism; D005054:Eugenol / Q000494:pharmacology*; D035264:Flowers; D046529:Microscopy, Electron, Transmission; D010572:Pest Control, Biological; D010935:Plant Diseases / Q000517:prevention & control*; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D043368:Ralstonia solanacearum / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism / Q000648:ultrastructure; D013385:Succinate Dehydrogenase / Q000378:metabolism; D031662:Syringa; D014026:Nicotiana
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D010936:Plant Extracts; D005054:Eugenol; D002374:Catalase; D013385:Succinate Dehydrogenase
keywords: Antibacterial mechanism; Eugenol; Ralstonia solanacearum; Syringa oblata