title: Growing Adipose-Derived Stem Cells Under Serum-Free Conditions.
abstract: Growing adipose-derived stem cells (ADSC) in serum-free conditions is important as it represents a way of expanding multipotent cells in a clinical grade medium. Most cultured ADSC are expanded and tested in serum-containing media, which can pose significant health risks if these cells were used in clinical applications. Moreover, cells grown in serum-free conditions behave significantly different than those cultured in serum-containing media. Here, we present a technique to culture adipose-derived stem cells in serum-free conditions. The methods described in this chapter were optimized for ovine ADSC. The appropriate optimization should be done for other cell lines.
authors.affiliation: Faculty of Dentistry, Sir John Walsh Research Institute, University of Otago, 310 Great King Street, Dunedin, 9016, New Zealand.
authors.forename: Diogo Godoy
authors.initials: DG
authors.lastname: Zanicotti
authors.affiliation: Faculty of Dentistry, Sir John Walsh Research Institute, University of Otago, 310 Great King Street, Dunedin, 9016, New Zealand. dawn.coates@otago.ac.nz.
authors.forename: Dawn E
authors.initials: DE
authors.lastname: Coates
mesh_terms: D000273:Adipose Tissue / Q000166:cytology*; D053687:Adult Stem Cells / Q000166:cytology* / Q000378:metabolism*; D000818:Animals; D018929:Cell Culture Techniques; D002469:Cell Separation; D016895:Culture Media, Serum-Free; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism
chemical_list: D016895:Culture Media, Serum-Free
keywords: Adipose-derived stem cells (ADSC); Fetal bovine serum (FBS); Mesenchymal stem cells (MSC); Ovine; Serum-free media