title: Submillimetre Network Formation by Light-induced Hybridization of Zeptomole-level DNA.
abstract: Macroscopic unique self-assembled structures are produced via double-stranded DNA formation (hybridization) as a specific binding essential in biological systems. However, a large amount of complementary DNA molecules are usually required to form an optically observable structure via natural hybridization, and the detection of small amounts of DNA less than femtomole requires complex and time-consuming procedures. Here, we demonstrate the laser-induced acceleration of hybridization between zeptomole-level DNA and DNA-modified nanoparticles (NPs), resulting in the assembly of a submillimetre network-like structure at the desired position with a dramatic spectral modulation within several minutes. The gradual enhancement of light-induced force and convection facilitated the two-dimensional network growth near the air-liquid interface with optical and fluidic symmetry breakdown. The simultaneous microscope observation and local spectroscopy revealed that the assembling process and spectral change are sensitive to the DNA sequence. Our findings establish innovative guiding principles for facile bottom-up production via various biomolecular recognition events.
authors.affiliation: Department of Physical Science, Graduate School of Science, Osaka Prefecture University, Sakai, Osaka 599-8570, Japan.|Nanoscience and Nanotechnology Research Center, Osaka Prefecture University, 1-2, Gakuencho, Nakaku, Sakai, Osaka 599-8570, Japan.
authors.forename: Takuya
authors.initials: T
authors.lastname: Iida
authors.affiliation: Department of Physical Science, Graduate School of Science, Osaka Prefecture University, Sakai, Osaka 599-8570, Japan.|Nanoscience and Nanotechnology Research Center, Osaka Prefecture University, 1-2, Gakuencho, Nakaku, Sakai, Osaka 599-8570, Japan.
authors.forename: Yushi
authors.initials: Y
authors.lastname: Nishimura
authors.affiliation: Department of Physical Science, Graduate School of Science, Osaka Prefecture University, Sakai, Osaka 599-8570, Japan.|Nanoscience and Nanotechnology Research Center, Osaka Prefecture University, 1-2, Gakuencho, Nakaku, Sakai, Osaka 599-8570, Japan.
authors.forename: Mamoru
authors.initials: M
authors.lastname: Tamura
authors.affiliation: Nanoscience and Nanotechnology Research Center, Osaka Prefecture University, 1-2, Gakuencho, Nakaku, Sakai, Osaka 599-8570, Japan.|Department of Applied Chemistry, Graduate School of Engineering, Osaka Prefecture University, 1-2, Gakuencho, Nakaku, Sakai, Osaka 599-8570, Japan.
authors.forename: Keisuke
authors.initials: K
authors.lastname: Nishida
authors.affiliation: Division of Frontier Materials Science, Graduate School of Engineering Science, Osaka University, 1-3, Machikaneyama, Toyonaka, Osaka 560-8531, Japan.
authors.forename: Syoji
authors.initials: S
authors.lastname: Ito
authors.affiliation: Nanoscience and Nanotechnology Research Center, Osaka Prefecture University, 1-2, Gakuencho, Nakaku, Sakai, Osaka 599-8570, Japan.|Department of Applied Chemistry, Graduate School of Engineering, Osaka Prefecture University, 1-2, Gakuencho, Nakaku, Sakai, Osaka 599-8570, Japan.
authors.forename: Shiho
authors.initials: S
authors.lastname: Tokonami
mesh_terms: D003198:Computer Simulation; D004247:DNA / Q000032:analysis / Q000737:chemistry*; D004058:Diffusion; D006046:Gold / Q000737:chemistry; D007834:Lasers; D008027:Light; D053768:Metal Nanoparticles / Q000737:chemistry; D008853:Microscopy; D015233:Models, Statistical; D053758:Nanoparticles / Q000737:chemistry*; D009690:Nucleic Acid Conformation; D009693:Nucleic Acid Hybridization; D017422:Sequence Analysis, DNA
chemical_list: D006046:Gold; D004247:DNA