title: [Rapid test for detection of susceptibility to cefotaxime in Enterobacteriaceae].
abstract: In this work an "in house" rapid test based on the change in pH that is due to hydrolysis for detecting Enterobacteriaceae susceptible to cefotaxime is evaluated. The strains of Enterobacteriaceae from 1947 urine cultures were assessed using MicroScan panels and the "in house" test. This rapid test includes red phenol solution and cefotaxime. Using MicroScan panels, 499 Enterobacteriaceae isolates were evaluated, which included 27 isolates of Escherichia coli producing extended-spectrum beta-lactamases (ESBL), 16 isolates of Klebsiella pneumoniae ESBL and 1 isolate of Klebsiella oxytoca ESBL. The "in house" test offers the following values: sensitivity 98% and specificity 97%, with negative predictive value 100% and positive predictive value 78%. The "in house" test based on the change of pH is useful in our area for detecting presumptively cefotaxime-resistant Enterobacteriaceae strains.
authors.affiliation: Laboratorio de Microbiología, Complejo Hospitalario Universitario de Granada-ibs, Granada, España.
authors.forename: Gemma
authors.initials: G
authors.lastname: Jiménez-Guerra
authors.affiliation: Laboratorio de Microbiología, Hospital Kremlin Bicetre, París, Francia.
authors.forename: Yannik
authors.initials: Y
authors.lastname: Hoyos-Mallecot
authors.affiliation: Laboratorio de Microbiología, Complejo Hospitalario Universitario de Granada-ibs, Granada, España.
authors.forename: Javier
authors.initials: J
authors.lastname: Rodríguez-Granger
authors.affiliation: Laboratorio de Microbiología, Complejo Hospitalario Universitario de Granada-ibs, Granada, España.
authors.forename: José María
authors.initials: JM
authors.lastname: Navarro-Marí
authors.affiliation: Departamento de Microbiología, Facultad de Medicina, Universidad de Granada-ibs, Granada, España. Electronic address: josegf@go.ugr.es.
authors.forename: José
authors.initials: J
authors.lastname: Gutiérrez-Fernández
mesh_terms: D002439:Cefotaxime / Q000494:pharmacology*; D003124:Colorimetry; D004396:Coloring Agents; D004755:Enterobacteriaceae / Q000187:drug effects* / Q000302:isolation & purification; D004756:Enterobacteriaceae Infections / Q000382:microbiology*; D004927:Escherichia coli Infections / Q000382:microbiology; D029968:Escherichia coli Proteins / Q000032:analysis; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D008565:Membrane Proteins / Q000032:analysis; D008826:Microbial Sensitivity Tests / Q000379:methods*; D010637:Phenolsulfonphthalein; D011237:Predictive Value of Tests; D012680:Sensitivity and Specificity; D014552:Urinary Tract Infections / Q000382:microbiology*; D056689:Uropathogenic Escherichia coli / Q000187:drug effects / Q000201:enzymology; D018440:beta-Lactam Resistance
chemical_list: C414316:Blr protein, E coli; D004396:Coloring Agents; D029968:Escherichia coli Proteins; D008565:Membrane Proteins; D010637:Phenolsulfonphthalein; D002439:Cefotaxime
keywords: Cefotaxima; Cefotaxime; Enterobacteriaceae; Enterobacterias; Extended spectrum β-lactamases; Pruebas rápidas; Rapid test; Red phenol; Rojo fenol; Urine culture; Urocultivos; β-lactamasas de espectro extendido