title: High Resolution Fiber-Fluorescence In Situ Hybridization.
abstract: High resolution fiber-Fluorescence in situ hybridization (FISH) is an advanced FISH technology that can effectively bridge the resolution gap between probe hybridizing on DNA molecules and chromosomal regions. Since various types of DNA and chromatin fibers can be generated reflecting different degrees of DNA/chromatin packaging status, fiber-FISH technology has been successfully used in diverse molecular cytogenetic/cytogenomic studies. Following a brief review of this technology, including its major development and increasing applications, typical protocols to generate DNA/chromatin fiber will be described, coupled with rationales, as well as technical tips. These released DNA/chromatin fibers are suitable for an array of cytogenetic/cytogenomic analyses.
authors.affiliation: The Division of Hematology/Oncology, Department of Internal Medicine, University of Michigan, Ann Arbor, MI, 48109, USA.
authors.forename: Christine J
authors.initials: CJ
authors.lastname: Ye
authors.affiliation: Center for Molecular Medicine and Genetics, Wayne State University School of Medicine, 3226 Scott Hall, 540 E, Detroit, MI, 48201, USA. hheng@med.wayne.edu.|Department of Pathology, Wayne State University School of Medicine, Detroit, MI, 48201, USA. hheng@med.wayne.edu.|Karmanos Cancer Institute, Detroit, MI, 48201, USA. hheng@med.wayne.edu.
authors.forename: Henry H
authors.initials: HH
authors.lastname: Heng
mesh_terms: D002478:Cells, Cultured; D002843:Chromatin / Q000235:genetics; D004247:DNA / Q000235:genetics; D015342:DNA Probes; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D008214:Lymphocytes / Q000378:metabolism; D008856:Microscopy, Fluorescence / Q000379:methods
chemical_list: D002843:Chromatin; D015342:DNA Probes; D004247:DNA
keywords: Chromatin fiber-FISH; DNA fiber-FISH; Free chromatin-FISH; Halo FISH; Stretched chromatin fiber-FISH