title: Activity of two extracts of Cynanchum paniculatum against Ichthyophthirius multifiliis theronts and tomonts.
abstract: The present study aims to evaluate the antiparasitic activity of active components from Cynanchum paniculatum against Ichthyophthirius multifiliis. The antiparasitic activities of two bioassay-guided fractionationated compounds from C. paniculatum identified as Cynatratoside-A and Cynanversicoside C, by comparing spectral data (NMR and ESI-MS) with literature values, were evaluated by in vitro assay. These showed that both could kill theronts of I. multifiliis at a concentration of 10·0 mg L-1, with the median effective concentration (EC50) values of 4·6 mg L-1 and 5·2 mg L-1 for Cynatratoside-A and Cynanversicoside C, respectively. Encysted tomonts were killed at concentrations of 8·0 mg L-1 with both compounds. In vivo experiments demonstrated that fish treated with both compounds at 15·0 mg L-1 carried significantly fewer parasites than controls (P < 0·05). There were no mortalities among treated fish group compared with 75% mortality of untreated fish. Cynatratoside-A and Cynanversicoside C are therefore potential candidate drugs for use against I. multifiliis.
authors.affiliation: First Hospital, Jilin University,Changchun, Jilin,China.
authors.forename: Wen
authors.initials: W
authors.lastname: Ji-Hong
authors.affiliation: First Hospital, Jilin University,Changchun, Jilin,China.
authors.forename: Wang
authors.initials: W
authors.lastname: Yan-Li
authors.affiliation: Hospital of Harbin Engineering University,Harbin,China.
authors.forename: Liu
authors.initials: L
authors.lastname: Yu-Hua
authors.affiliation: College of optical And Electronical Information Changchun University of Science and Technology, Changchun,Jilin,China.
authors.forename: Zhang
authors.initials: Z
authors.lastname: Ji-Yuan
authors.affiliation: Jilin Agriculture University,2888 Xincheng Road, Changchun, Jilin,China.
authors.forename: L I
authors.initials: LI
authors.lastname: Ze-Hong
mesh_terms: D000871:Anthelmintics / Q000737:chemistry / Q000494:pharmacology*; D016798:Ciliophora / Q000187:drug effects*; D031105:Cynanchum / Q000737:chemistry*; D009281:Naphthalenes / Q000737:chemistry / Q000494:pharmacology*; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*
chemical_list: D000871:Anthelmintics; D009281:Naphthalenes; D010936:Plant Extracts; C000623095:cynanversicoside C; C078993:cynatratoside A
keywords: 
                 Cynanchum paniculatum
              ; 
                 Ichthyophthirius multifiliis
              ; Cynanversicoside C; Cynatratoside-A; plant extract; treatment