title: Primary Postnatal Dorsal Root Ganglion Culture from Conventionally Slaughtered Calves.
abstract: Neurological disorders in ruminants have an important impact on veterinary health, but very few host-specific in vitro models have been established to study diseases affecting the nervous system. Here we describe a primary neuronal dorsal root ganglia (DRG) culture derived from calves after being conventionally slaughtered for food consumption. The study focuses on the in vitro characterization of bovine DRG cell populations by immunofluorescence analysis. The effects of various growth factors on neuron viability, neurite outgrowth and arborisation were evaluated by morphological analysis. Bovine DRG neurons are able to survive for more than 4 weeks in culture. GF supplementation is not required for neuronal survival and neurite outgrowth. However, exogenously added growth factors promote neurite outgrowth. DRG cultures from regularly slaughtered calves represent a promising and sustainable host specific model for the investigation of pain and neurological diseases in bovines.
authors.affiliation: Division of Neurological Sciences, Vetsuisse Faculty, University of Bern, Bern, Switzerland.|Graduate School for Cellular and Biomedical Sciences, Theodor Kocher Institute, University of Bern, Switzerland.
authors.forename: A
authors.initials: A
authors.lastname: Fadda
authors.affiliation: Division of Neurological Sciences, Vetsuisse Faculty, University of Bern, Bern, Switzerland.
authors.forename: M
authors.initials: M
authors.lastname: Bärtschi
authors.affiliation: Institute for Parasitology, Vetsuisse Faculty, University of Bern, Bern, Switzerland.
authors.forename: A
authors.initials: A
authors.lastname: Hemphill
authors.affiliation: Neurocenter and Regenerative Neuroscience Cluster, University Hospital and University of Bern, Bern, Switzerland.
authors.forename: H R
authors.initials: HR
authors.lastname: Widmer
authors.affiliation: Division of Neurological Sciences, Vetsuisse Faculty, University of Bern, Bern, Switzerland.
authors.forename: A
authors.initials: A
authors.lastname: Zurbriggen
authors.affiliation: School of Engineering, The University of Edinburgh, Edinburgh, United Kingdom.
authors.forename: P
authors.initials: P
authors.lastname: Perona
authors.affiliation: Veterinary Public Health Institute (VPHI), Vetsuisse Faculty, University of Bern, Bern, Switzerland.
authors.forename: B
authors.initials: B
authors.lastname: Vidondo
authors.affiliation: Division of Neurological Sciences, Vetsuisse Faculty, University of Bern, Bern, Switzerland.
authors.forename: A
authors.initials: A
authors.lastname: Oevermann
mesh_terms: D000818:Animals; D002417:Cattle; D002478:Cells, Cultured; D018920:Coculture Techniques; D005727:Ganglia, Spinal / Q000473:pathology*; D046529:Microscopy, Electron, Transmission