title: Synthesis of a molecularly imprinted polymer for the selective recognition of carmoisine (Azorubin E122) from pomegranate juice.
abstract: Since natural pigments are lost during the processing of beverages such as pomegranate juice, carmoisine, as an adulterant, is often added into the pure juice to improve color characteristics. In this study, molecularly imprinted polymers, as an adsorbent of carmoisine, were synthesized using acrylamide, methacrylic acid, and 4-vinylpyridine as functional monomers and then they were evaluated in terms of the separation and detection of carmoisine. Experiments on the batch adsorption of carmoisine 10 ppm stock solution revealed a better binding capacity for the 4-vinylpyridine-based polymer in comparison to methacrylic acid and acrylamide polymers. The complexation of carmoisine with the 4-vinylpyridine-based polymer was confirmed by Fourier transform infrared spectroscopy. The synthesized polymer exerted a high thermal degradation point and average diameter of polymer particles were obtained to be 0.2 μm by dynamic light scattering analysis. This work showed that detection of pomegranate juice adulteration with carmoisine is not necessarily difficult, time consuming or expensive with selective separation techniques such as molecularly imprinted polymers.
authors.affiliation: Department of Food Science and Technology, Faculty of Agriculture, Urmia University, Urmia, West Azerbaijan, Iran.
authors.forename: Zahra
authors.initials: Z
authors.lastname: Ghasempour
authors.affiliation: Department of Food Science and Technology, Faculty of Agriculture, Urmia University, Urmia, West Azerbaijan, Iran.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Alizadeh-Khaledabad
authors.affiliation: Department of Medicinal Chemistry, Faculty of Pharmacy, Urmia University of Medical Sciences, Urmia, West Azerbaijan, Iran.
authors.forename: Mohammad-Reza
authors.initials: MR
authors.lastname: Vardast
authors.affiliation: Department of Food Science and Technology, Faculty of Agriculture, Urmia University, Urmia, West Azerbaijan, Iran.
authors.forename: Mahmoud
authors.initials: M
authors.lastname: Rezazad-Bari
mesh_terms: D000327:Adsorption; D000067030:Fruit and Vegetable Juices / Q000032:analysis; D029561:Lythraceae; D009282:Naphthalenesulfonates / Q000032:analysis* / Q000302:isolation & purification* / Q000378:metabolism; D011108:Polymers / Q000138:chemical synthesis* / Q000378:metabolism
chemical_list: D009282:Naphthalenesulfonates; D011108:Polymers; C006671:azo rubin S
keywords: 4-vinylpyridine; carmoisine; molecularly imprinted polymers; pomegranate juice