title: Targeting Conserved Genes in Penicillium Species.
abstract: Polymerase chain reaction amplification of conserved genes and sequence analysis provides a very powerful tool for the identification of toxigenic as well as non-toxigenic Penicillium species. Sequences are obtained by amplification of the gene fragment, sequencing via capillary electrophoresis of dideoxynucleotide-labeled fragments or NGS. The sequences are compared to a database of validated isolates. Identification of species indicates the potential of the fungus to make particular mycotoxins.
authors.affiliation: Bacterial Foodborne Pathogens and Mycology Research Unit, National Center for Agricultural Utilization Research, Agricultural Research Service, U. S. Department of Agriculture, 1815 North University Street, Peoria, IL, 61604, USA. Stephen.Peterson@ARS.USDA.GOV.
authors.forename: Stephen W
authors.initials: SW
authors.lastname: Peterson
mesh_terms: D019295:Computational Biology / Q000379:methods; D017124:Conserved Sequence; D058893:DNA Barcoding, Taxonomic; D021903:DNA, Ribosomal Spacer / Q000235:genetics; D030561:Databases, Nucleic Acid; D019143:Evolution, Molecular; D020043:Genes, Essential; D005800:Genes, Fungal; D010407:Penicillium / Q000145:classification* / Q000235:genetics*; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D012984:Software; D064878:Web Browser
chemical_list: D021903:DNA, Ribosomal Spacer
keywords: Barcode; DNA sequencing; Housekeeping genes; ITS; Identification