title: Label-Free Virus Capture and Release by a Microfluidic Device Integrated with Porous Silicon Nanowire Forest.
abstract: Viral diseases are perpetual threats to human and animal health. Detection and characterization of viral pathogens require accurate, sensitive, and rapid diagnostic assays. For field and clinical samples, the sample preparation procedures limit the ultimate performance and utility of the overall virus diagnostic protocols. This study presents the development of a microfluidic device embedded with porous silicon nanowire (pSiNW) forest for label-free size-based point-of-care virus capture in a continuous curved flow design. The pSiNW forests with specific interwire spacing are synthesized in situ on both bottom and sidewalls of the microchannels in a batch process. With the enhancement effect of Dean flow, this study demonstrates that about 50% H5N2 avian influenza viruses are physically trapped without device clogging. A unique feature of the device is that captured viruses can be released by inducing self-degradation of the pSiNWs in physiological aqueous environment. About 60% of captured viruses can be released within 24 h for virus culture, subsequent molecular diagnosis, and other virus characterization and analyses. This device performs viable, unbiased, and label-free virus isolation and release. It has great potentials for virus discovery, virus isolation and culture, functional studies of virus pathogenicity, transmission, drug screening, and vaccine development.
authors.affiliation: Department of Biomedical Engineering, Micro and Nano Integrated Biosystem (MINIBio) Laboratory, The Pennsylvania State University, University Park, PA, 16802, USA.
authors.forename: Yiqiu
authors.initials: Y
authors.lastname: Xia
authors.affiliation: Department of Veterinary and Biomedical Science, The Pennsylvania State University, University Park, PA, 16802, USA.
authors.forename: Yi
authors.initials: Y
authors.lastname: Tang
authors.affiliation: Department of Biomedical Engineering, Micro and Nano Integrated Biosystem (MINIBio) Laboratory, The Pennsylvania State University, University Park, PA, 16802, USA.
authors.forename: Xu
authors.initials: X
authors.lastname: Yu
authors.affiliation: Department of Biomedical Engineering, Micro and Nano Integrated Biosystem (MINIBio) Laboratory, The Pennsylvania State University, University Park, PA, 16802, USA.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Wan
authors.affiliation: Department of Electrical Engineering, The Pennsylvania State University, University Park, PA, 16802, USA.
authors.forename: Yizhu
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Department of Veterinary and Biomedical Science, The Pennsylvania State University, University Park, PA, 16802, USA.
authors.forename: Huaguang
authors.initials: H
authors.lastname: Lu
authors.affiliation: Department of Biomedical Engineering, Micro and Nano Integrated Biosystem (MINIBio) Laboratory, The Pennsylvania State University, University Park, PA, 16802, USA.|Department of Electrical Engineering, The Pennsylvania State University, University Park, PA, 16802, USA.|Material Research Institute, The Pennsylvania State University, University Park, PA, 16802, USA.|Huck Institutes of Life Sciences, The Pennsylvania State University, University Park, PA, 16802, USA.
authors.forename: Si-Yang
authors.initials: SY
authors.lastname: Zheng
mesh_terms: D004867:Equipment Design; D009980:Influenza A virus / Q000378:metabolism*; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*; D054118:Nanospheres / Q000737:chemistry; D053770:Nanowires / Q000737:chemistry*; D010316:Particle Size; D016062:Porosity; D012825:Silicon / Q000737:chemistry*; D013194:Staining and Labeling
chemical_list: D012825:Silicon
keywords: Dean flow; label free; point of care; porous silicon nanowire; virus capture