title: Dengue Virus Isolation in Mosquito Aedes albopictus Captured During an Outbreak in Tokyo, 2014, by a Method Relying on Antibody-Dependent Enhancement Mechanism Using FcγR-Expressing BHK Cells.
abstract: Dengue virus (DENV) isolation from mosquitoes is necessary for providing definitive evidence of virus circulation, and is critical for further virological characterization and determination of epidemiological characteristics. By using Aedes albopictus mosquitoes captured during an outbreak in Tokyo in 2014, we compared the DENV isolation rates of a conventional virus isolation method that uses C6/36 mosquito cells as assay cells with those of a virus isolation method that relies on an antibody-dependent enhancement (ADE) mechanism by using FcγR-expressing baby hamster kidney (BHK) cells and an antibody with ADE activity. The number of DENV genome copies and infectious virus titers in cell culture supernatant fluids of FcγR-expressing BHK cells were significantly higher than those of the C6/36 cells. In addition, DENV was isolated from a mosquito pool by using FcγR-expressing BHK cells only in the presence of infection-enhancing antibody. Infectious virus was detected in six mosquito pools only by using FcγR-expressing BHK cells. The results suggest that the method that relies on ADE mechanism by using the FcγR-expressing BHK cells and an antibody with ADE activity is useful for DENV isolation from mosquitoes caught in the field.
authors.affiliation: 1 Institute of Tropical Medicine, Nagasaki University , Nagasaki, Japan .|2 Department of Virology 1, National Institute of Infectious Diseases , Tokyo, Japan .
authors.forename: Meng Ling
authors.initials: ML
authors.lastname: Moi
authors.affiliation: 3 Department of Medical Entomology, National Institute of Infectious Diseases , Tokyo, Japan .
authors.forename: Daisuke
authors.initials: D
authors.lastname: Kobayashi
authors.affiliation: 3 Department of Medical Entomology, National Institute of Infectious Diseases , Tokyo, Japan .
authors.forename: Haruhiko
authors.initials: H
authors.lastname: Isawa
authors.affiliation: 3 Department of Medical Entomology, National Institute of Infectious Diseases , Tokyo, Japan .
authors.forename: Toshinori
authors.initials: T
authors.lastname: Sasaki
authors.affiliation: 2 Department of Virology 1, National Institute of Infectious Diseases , Tokyo, Japan .
authors.forename: Masayuki
authors.initials: M
authors.lastname: Saijo
authors.affiliation: 4 National Institute of Infectious Diseases , Tokyo, Japan .
authors.forename: Ichiro
authors.initials: I
authors.lastname: Kurane
authors.affiliation: 3 Department of Medical Entomology, National Institute of Infectious Diseases , Tokyo, Japan .
authors.forename: Kyoko
authors.initials: K
authors.lastname: Sawabe
authors.affiliation: 2 Department of Virology 1, National Institute of Infectious Diseases , Tokyo, Japan .|5 Kanagawa Prefectural Institute of Public Health , Kanagawa, Japan .
authors.forename: Tomohiko
authors.initials: T
authors.lastname: Takasaki
mesh_terms: D000330:Aedes / Q000821:virology*; D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology*; D002460:Cell Line; D006224:Cricetinae; D003715:Dengue / Q000453:epidemiology* / Q000821:virology*; D003716:Dengue Virus / Q000302:isolation & purification*; D016679:Genome, Viral; D006801:Humans; D000072138:Mosquito Vectors; D017452:Receptors, IgG / Q000235:genetics / Q000378:metabolism*
chemical_list: D000914:Antibodies, Viral; D017452:Receptors, IgG
keywords: Aedes albopictus; FcγR-expressing cells; antibody-dependent enhancement; dengue virus; mosquito; virus isolation