title: Molecular Characterization of Resistance to Soybean Rust (Phakopsora pachyrhizi Syd. & Syd.) in Soybean Cultivar DT 2000 (PI 635999).
abstract: Resistance to soybean rust (SBR), caused by Phakopsora pachyrhizi Syd. & Syd., has been identified in many soybean germplasm accessions and is conferred by either dominant or recessive genes that have been mapped to six independent loci (Rpp1 -Rpp6), but No U.S. cultivars are resistant to SBR. The cultivar DT 2000 (PI 635999) has resistance to P. pachyrhizi isolates and field populations from the United States as well as Vietnam. A F6:7 recombinant inbred line (RIL) population derived from Williams 82 × DT 2000 was used to identify genomic regions associated with resistance to SBR in the field in Ha Noi, Vietnam, and in Quincy, Florida, in 2008. Bulked segregant analysis (BSA) was conducted using the soybean single nucleotide polymorphism (SNP) USLP 1.0 panel along with simple sequence repeat (SSR) markers to detect regions of the genome associated with resistance. BSA identified four BARC_SNP markers near the Rpp3 locus on chromosome (Chr.) 6. Genetic analysis identified an additional genomic region around the Rpp4 locus on Chr. 18 that was significantly associated with variation in the area under disease progress curve (AUDPC) values and sporulation in Vietnam. Molecular markers tightly linked to the DT 2000 resistance alleles on Chrs. 6 and 18 will be useful for marker-assisted selection and backcrossing in order to pyramid these genes with other available SBR resistance genes to develop new varieties with enhanced and durable resistance to SBR.
authors.affiliation: Division of Plant Sciences, University of Missouri, Columbia, Missouri, United States of America.
authors.forename: Tri D
authors.initials: TD
authors.lastname: Vuong
authors.affiliation: Soybean/Maize Germplasm, Pathology, and Genetics Research Unit, USDA-ARS, and Department of Crop Sciences, University of Illinois, Urbana, Illinois,United States of America.
authors.forename: David R
authors.initials: DR
authors.lastname: Walker
authors.affiliation: Plant Protection Research Institute (PPRI), Ha Noi, Vietnam.
authors.forename: Binh T
authors.initials: BT
authors.lastname: Nguyen
authors.affiliation: Plant Protection Research Institute (PPRI), Ha Noi, Vietnam.
authors.forename: Tuyet T
authors.initials: TT
authors.lastname: Nguyen
authors.affiliation: Plant Protection Research Institute (PPRI), Ha Noi, Vietnam.
authors.forename: Hoan X
authors.initials: HX
authors.lastname: Dinh
authors.affiliation: Soybean Genomics and Improvement Laboratory, USDA-ARS, Beltsville, Maryland, United States of America.
authors.forename: David L
authors.initials: DL
authors.lastname: Hyten
authors.affiliation: Soybean Genomics and Improvement Laboratory, USDA-ARS, Beltsville, Maryland, United States of America.
authors.forename: Perry B
authors.initials: PB
authors.lastname: Cregan
authors.affiliation: Division of Plant Sciences, University of Missouri, Columbia, Missouri, United States of America.
authors.forename: David A
authors.initials: DA
authors.lastname: Sleper
authors.affiliation: Division of Plant Sciences, University of Missouri, Portageville, Missouri, United States of America.
authors.forename: Jeong D
authors.initials: JD
authors.lastname: Lee
authors.affiliation: Division of Plant Sciences, University of Missouri, Columbia, Missouri, United States of America.
authors.forename: James G
authors.initials: JG
authors.lastname: Shannon
authors.affiliation: Division of Plant Sciences, University of Missouri, Columbia, Missouri, United States of America.
authors.forename: Henry T
authors.initials: HT
authors.lastname: Nguyen
mesh_terms: D000483:Alleles; D002874:Chromosome Mapping; D032461:Chromosomes, Plant / Q000737:chemistry*; D060467:Disease Resistance / Q000235:genetics; D056426:Genetic Loci; D005819:Genetic Markers / Q000276:immunology; D018745:Genome, Plant; D005838:Genotype; D018895:Microsatellite Repeats / Q000276:immunology; D000067563:Phakopsora pachyrhizi / Q000472:pathogenicity / Q000502:physiology*; D010935:Plant Diseases / Q000235:genetics* / Q000276:immunology / Q000382:microbiology; D020641:Polymorphism, Single Nucleotide; D013025:Glycine max / Q000235:genetics* / Q000276:immunology / Q000382:microbiology; D013172:Spores, Fungal / Q000472:pathogenicity / Q000502:physiology*
chemical_list: D005819:Genetic Markers