title: N2O emission characteristics and its affecting factors in rain-fed potato fields in Wuchuan County, China.
abstract: Representing an important greenhouse gas, nitrous oxide (N2O) emission from cultivated land is a hot topic in current climate change research. This study examined the influences of nitrogen fertilisation, temperature and soil moisture on the ammonia monooxygenase subunit A (amoA) gene copy numbers and N2O emission characteristics. The experimental observation of N2O fluxes was based on the static chamber-gas chromatographic method. The ammonia-oxidising bacteria (AOB) and ammonia-oxidising archaea (AOA) gene copy numbers in different periods were measured by real-time polymerase chain reaction (PCR). The results indicated that rain-fed potato field was a N2O source, and the average annual N2O emission was approximately 0.46 ± 0.06 kgN2O-N/ha/year. N2O emissions increased significantly with increase in fertilisation, temperatures below 19.6 °C and soil volumetric water content under 15%. Crop rotation appreciably decreases N2O emissions by 34.4 to 52.4% compared to continuous cropping in rain-fed potato fields. The significant correlation between N2O fluxes and AOB copy numbers implied that N2O emissions were primarily controlled by AOB in rain-fed potato fields. The research has important theoretical and practical value for understanding N2O emissions from rain-fed dry farmland fields.
authors.affiliation: College of Resources and Environmental Science, China Agricultural University, No.2 Yuanmingyuan West Road, Haidian, Beijing, 100193, People's Republic of China.|College of Agronomy, Shenyang Agricultural University, Shenyang, 110866, China.|Wuchuan Scientific Observing and Experimental Station of Agro-Environment, Ministry of Agriculture, Wuchuan, 011700, China.
authors.forename: Liwei
authors.initials: L
authors.lastname: Wang
authors.affiliation: Corn Research Institute of Liaoning Academy of Agricultural Sciences, Shenyang, 110161, China.
authors.forename: Cheng
authors.initials: C
authors.lastname: Wang
authors.affiliation: College of Resources and Environmental Science, China Agricultural University, No.2 Yuanmingyuan West Road, Haidian, Beijing, 100193, People's Republic of China. panzhihua@cau.edu.cn.|Wuchuan Scientific Observing and Experimental Station of Agro-Environment, Ministry of Agriculture, Wuchuan, 011700, China. panzhihua@cau.edu.cn.
authors.forename: Zhihua
authors.initials: Z
authors.lastname: Pan
authors.affiliation: Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang, 110016, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Xu
authors.affiliation: School of Resources and Environmental, Anhui Agricultural University, Hefei, 230036, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Gao
authors.affiliation: Wuchuan Scientific Observing and Experimental Station of Agro-Environment, Ministry of Agriculture, Wuchuan, 011700, China.|Institute of Resources Environmental and Detection Technology, Inner Mongolia Academy of Agricultural and Animal Husbandry Sciences, Huhhot, 010031, China.
authors.forename: Peiyi
authors.initials: P
authors.lastname: Zhao
authors.affiliation: College of Resources and Environmental Science, China Agricultural University, No.2 Yuanmingyuan West Road, Haidian, Beijing, 100193, People's Republic of China.|Wuchuan Scientific Observing and Experimental Station of Agro-Environment, Ministry of Agriculture, Wuchuan, 011700, China.
authors.forename: Zhiqiang
authors.initials: Z
authors.lastname: Dong
authors.affiliation: College of Resources and Environmental Science, China Agricultural University, No.2 Yuanmingyuan West Road, Haidian, Beijing, 100193, People's Republic of China.|Wuchuan Scientific Observing and Experimental Station of Agro-Environment, Ministry of Agriculture, Wuchuan, 011700, China.
authors.forename: Jingting
authors.initials: J
authors.lastname: Zhang
authors.affiliation: College of Resources and Environmental Science, China Agricultural University, No.2 Yuanmingyuan West Road, Haidian, Beijing, 100193, People's Republic of China.|Wuchuan Scientific Observing and Experimental Station of Agro-Environment, Ministry of Agriculture, Wuchuan, 011700, China.
authors.forename: Guohui
authors.initials: G
authors.lastname: Cui
authors.affiliation: College of Resources and Environmental Science, China Agricultural University, No.2 Yuanmingyuan West Road, Haidian, Beijing, 100193, People's Republic of China.|Wuchuan Scientific Observing and Experimental Station of Agro-Environment, Ministry of Agriculture, Wuchuan, 011700, China.
authors.forename: Sen
authors.initials: S
authors.lastname: Wang
authors.affiliation: College of Resources and Environmental Science, China Agricultural University, No.2 Yuanmingyuan West Road, Haidian, Beijing, 100193, People's Republic of China.|Wuchuan Scientific Observing and Experimental Station of Agro-Environment, Ministry of Agriculture, Wuchuan, 011700, China.
authors.forename: Guolin
authors.initials: G
authors.lastname: Han
authors.affiliation: College of Resources and Environmental Science, China Agricultural University, No.2 Yuanmingyuan West Road, Haidian, Beijing, 100193, People's Republic of China.|Wuchuan Scientific Observing and Experimental Station of Agro-Environment, Ministry of Agriculture, Wuchuan, 011700, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Zhao
mesh_terms: D000383:Agriculture / Q000379:methods; D000393:Air Pollutants / Q000032:analysis*; D000641:Ammonia / Q000378:metabolism; D001105:Archaea / Q000235:genetics / Q000378:metabolism; D001419:Bacteria / Q000235:genetics / Q000378:metabolism; D002681:China; D005308:Fertilizers; D018628:Gene Dosage; D019766:Genes, Archaeal; D005798:Genes, Bacterial; D009609:Nitrous Oxide / Q000032:analysis*; D010084:Oxidation-Reduction; D010088:Oxidoreductases / Q000235:genetics; D011891:Rain; D012988:Soil Microbiology; D011198:Solanum tuberosum; D013696:Temperature
chemical_list: D000393:Air Pollutants; D005308:Fertilizers; D000641:Ammonia; D010088:Oxidoreductases; C045582:ammonia monooxygenase; D009609:Nitrous Oxide
keywords: Ammonia-oxidising bacteria; Crop patterns; N2O emission; Nitrogen fertiliser