title: Molecular diversity of human parvovirus B19 during two outbreaks of erythema infectiosum in Brazil.
abstract: This study was conducted to provide information on the genetic diversity of human parvovirus B19 (B19V) circulating in the municipality of Niterói, Rio de Janeiro, Southeast Brazil during 1996-2006, a period with two distinct outbreaks of B19V infection: 1999-2000 and 2004-2005. A total of 27 sera from patients with erythema infectiosum and five sera from HIV-infected patients that tested positive for B19V DNA during the study period were analyzed. To genotype B19V strains, a semi-nested PCR for partial amplification of the capsid gene was performed and sequence analysis revealed that 31 sequences belonged to subgenotype 1a (G1a) of the main genotype 1 and one sequence was characterized as subgenotype 3b (G3b). The phylogenetic tree supported the division of the G1a into two well-defined clades with 1.3% of divergence. The low diversity of the G1a strains may be explained by the fact that all patients had acute B19V infection and 30/32 sera were collected during two distinct outbreaks. The G3b strain was from an HIV-infected patient who seroconverted to anti-B19 IgG antibodies in September/2005. This is the first report of G3b in the state of Rio de Janeiro.
authors.affiliation: Universidade Federal Fluminense, Instituto Biomédico, Departamento de Microbiologia e Parasitologia, Niterói, RJ, Brazil. Electronic address: ritacubel@id.uff.br.
authors.forename: Rita de Cássia Nasser
authors.initials: RC
authors.lastname: Cubel Garcia
authors.affiliation: Universidade Federal Fluminense, Instituto Biomédico, Departamento de Microbiologia e Parasitologia, Niterói, RJ, Brazil; Universidade Federal Fluminense, Faculdade de Medicina, Programa de Pós Graduação em Ciências Médicas, Niterói, RJ, Brazil.
authors.forename: Renata Freire Alves
authors.initials: RF
authors.lastname: Pereira
authors.affiliation: Universidade Federal Fluminense, Faculdade de Medicina, Disciplina de Doenças Infecciosas e Parasitárias, Niterói, RJ, Brazil.
authors.forename: Kátia Martins Lopes de
authors.initials: KM
authors.lastname: Azevedo
authors.affiliation: Universidade Federal Fluminense, Instituto Biomédico, Departamento de Microbiologia e Parasitologia, Niterói, RJ, Brazil.
authors.forename: Tatiana Xavier de
authors.initials: TX
authors.lastname: Castro
authors.affiliation: Universidade Federal Fluminense, Instituto Biomédico, Departamento de Microbiologia e Parasitologia, Niterói, RJ, Brazil.
authors.forename: Francisco C A
authors.initials: FC
authors.lastname: Mello
authors.affiliation: Universidade Federal Fluminense, Faculdade de Medicina, Disciplina de Doenças Infecciosas e Parasitárias, Niterói, RJ, Brazil.
authors.forename: Sérgio
authors.initials: S
authors.lastname: Setubal
authors.affiliation: Fiocruz, Instituto Oswaldo Cruz, Laboratório de Vírus Respiratórios e Sarampo, Rio de Janeiro, RJ, Brazil.
authors.forename: Marilda M
authors.initials: MM
authors.lastname: Siqueira
authors.affiliation: Fiocruz, Instituto Oswaldo Cruz, Laboratório de Vírus Respiratórios e Sarampo, Rio de Janeiro, RJ, Brazil.
authors.forename: David
authors.initials: D
authors.lastname: Brown
authors.affiliation: Universidade Federal Fluminense, Faculdade de Medicina, Programa de Pós Graduação em Ciências Médicas, Niterói, RJ, Brazil; Universidade Federal Fluminense, Faculdade de Medicina, Disciplina de Doenças Infecciosas e Parasitárias, Niterói, RJ, Brazil.
authors.forename: Solange Artimos de
authors.initials: SA
authors.lastname: Oliveira
mesh_terms: D000293:Adolescent; D000328:Adult; D001938:Brazil / Q000453:epidemiology; D002648:Child; D004196:Disease Outbreaks; D016731:Erythema Infectiosum / Q000453:epidemiology* / Q000235:genetics / Q000821:virology*; D005260:Female; D005838:Genotype; D006801:Humans; D008297:Male; D008875:Middle Aged; D016732:Parvovirus B19, Human / Q000235:genetics*; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D055815:Young Adult
keywords: B19V genotypes; Brazil; Erythema infectiosum; Outbreak