title: A new isoquinolone alkaloid from an endophytic fungus R22 of Nerium indicum.
abstract: A new isoquinolone alkaloid named 5-hydroxy-8-methoxy-4-phenylisoquinolin-1(2H)-one (3), together with two known quinolinone alkaloids 3-O-methylviridicatin (1) and viridicatol (2) were isolated from the fermentation of an endophytic fungus Penicillium sp. R22 in Nerium indicum. Their structures were elucidated by NMR, IR and MS data, and were also confirmed by comparing with the reported data in the literature. Meanwhile, the antibacterial and antifungal activities of all compounds were tested, and the results showed that three compounds had strong antifungal activity. Among them, compound 2 revealed potent antibacterial activity against Staphylococcus aureus with MIC value of 15.6 μg/mL.
authors.affiliation: a Key Laboratory of Auxiliary Chemistry & Technology for Chemical Industry, Ministry of Education , Shaanxi University of Science and Technology , Shaanxi Xi'an , China.
authors.forename: Yang-Min
authors.initials: YM
authors.lastname: Ma
authors.affiliation: a Key Laboratory of Auxiliary Chemistry & Technology for Chemical Industry, Ministry of Education , Shaanxi University of Science and Technology , Shaanxi Xi'an , China.
authors.forename: Ke
authors.initials: K
authors.lastname: Qiao
authors.affiliation: a Key Laboratory of Auxiliary Chemistry & Technology for Chemical Industry, Ministry of Education , Shaanxi University of Science and Technology , Shaanxi Xi'an , China.|b Institute of Food and Biological Engineering , Shaanxi University of Science and Technology , Shaanxi Xi'an , China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Kong
authors.affiliation: a Key Laboratory of Auxiliary Chemistry & Technology for Chemical Industry, Ministry of Education , Shaanxi University of Science and Technology , Shaanxi Xi'an , China.
authors.forename: Meng-Yun
authors.initials: MY
authors.lastname: Li
authors.affiliation: a Key Laboratory of Auxiliary Chemistry & Technology for Chemical Industry, Ministry of Education , Shaanxi University of Science and Technology , Shaanxi Xi'an , China.
authors.forename: Lin-Xin
authors.initials: LX
authors.lastname: Guo
authors.affiliation: a Key Laboratory of Auxiliary Chemistry & Technology for Chemical Industry, Ministry of Education , Shaanxi University of Science and Technology , Shaanxi Xi'an , China.
authors.forename: Zhi
authors.initials: Z
authors.lastname: Miao
authors.affiliation: a Key Laboratory of Auxiliary Chemistry & Technology for Chemical Industry, Ministry of Education , Shaanxi University of Science and Technology , Shaanxi Xi'an , China.
authors.forename: Chao
authors.initials: C
authors.lastname: Fan
mesh_terms: D000470:Alkaloids / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology*; D000935:Antifungal Agents / Q000737:chemistry / Q000494:pharmacology*; D004353:Drug Evaluation, Preclinical / Q000379:methods; D060026:Endophytes / Q000737:chemistry; D006912:Hydroxyquinolines / Q000737:chemistry / Q000302:isolation & purification; D007546:Isoquinolines / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D008826:Microbial Sensitivity Tests; D015394:Molecular Structure; D030007:Nerium / Q000382:microbiology*; D010407:Penicillium / Q000737:chemistry* / Q000502:physiology; D015363:Quinolones / Q000737:chemistry / Q000302:isolation & purification; D013211:Staphylococcus aureus / Q000187:drug effects
chemical_list: C116810:3-O-methylviridicatin; C000619396:5-hydroxy-8-methoxy-4-phenylisoquinolin-1(2H)-one; D000470:Alkaloids; D000900:Anti-Bacterial Agents; D000935:Antifungal Agents; D006912:Hydroxyquinolines; D007546:Isoquinolines; D015363:Quinolones; C108793:viridicatol
keywords: 5-hydroxy-8-methoxy-4-phenylisoquinolin-1(2H)-one; Nerium indicum; Penicillium sp; Quinoline