title: Circulating MicroRNA Are Predictive of Aging and Acute Adaptive Response to Resistance Exercise in Men.
abstract: Circulating microRNA (c-miRNA) have the potential to function as novel noninvasive markers of the underlying physiological state of skeletal muscle. This investigation sought to determine the influence of aging on c-miRNA expression at rest and following resistance exercise in male volunteers (Young: n = 9; Older: n = 9). Primary findings were that fasting c-miRNA expression profiles were significantly predictive of aging, with miR-19b-3p, miR-206, and miR-486 distinguishing between age groups. Following resistance exercise, principal component analysis revealed a divergent response in expression of 10 c-miRNA, where expression profiles were upregulated in younger and downregulated in older participants. Using Ingenuity Pathway Analysis to test c-miRNA-to-mRNA interactions in skeletal muscle, it was found that response of c-miRNA to exercise was indicative of an anabolic response in younger but not older participants. These findings were corroborated with a positive association observed with the phosphorylation status of p-AktSer473 and p-S6K1Thr389 and expression of miR-19a-3p, miR-19b-3p, miR-20a-5p, miR-26b-5p, miR-143-3p, and miR-195-5p. These important findings provide compelling evidence that dysregulation of c-miRNA expression with aging may not only serve as a predictive marker, but also reflect underlying molecular mechanisms resulting in age-associated declines in skeletal muscle mass, increased fat mass, and "anabolic resistance."
authors.affiliation: Nutrition, Exercise, Physiology, and Sarcopenia Laboratory, Jean Mayer U.S. Department of Agriculture Human Nutrition Research Center on Aging, Tufts University, Boston, Massachusetts.
authors.forename: Lee M
authors.initials: LM
authors.lastname: Margolis
authors.affiliation: Section of Clinical Research, Joslin Diabetes Center.|Brigham and Women's Hospital.|Department of Medicine, Harvard Medical School, Boston, Massachusetts.
authors.forename: Sarah J
authors.initials: SJ
authors.lastname: Lessard
authors.affiliation: Nutrition, Exercise, Physiology, and Sarcopenia Laboratory, Jean Mayer U.S. Department of Agriculture Human Nutrition Research Center on Aging, Tufts University, Boston, Massachusetts.
authors.forename: Yassine
authors.initials: Y
authors.lastname: Ezzyat
authors.affiliation: Nutrition, Exercise, Physiology, and Sarcopenia Laboratory, Jean Mayer U.S. Department of Agriculture Human Nutrition Research Center on Aging, Tufts University, Boston, Massachusetts.
authors.forename: Roger A
authors.initials: RA
authors.lastname: Fielding
authors.affiliation: Nutrition, Exercise, Physiology, and Sarcopenia Laboratory, Jean Mayer U.S. Department of Agriculture Human Nutrition Research Center on Aging, Tufts University, Boston, Massachusetts.
authors.forename: Donato A
authors.initials: DA
authors.lastname: Rivas
mesh_terms: D000222:Adaptation, Physiological / Q000502:physiology*; D000368:Aged; D000375:Aging / Q000502:physiology*; D000886:Anthropometry; D015415:Biomarkers / Q000378:metabolism; D001823:Body Composition; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000378:metabolism*; D018482:Muscle, Skeletal / Q000378:metabolism*; D011237:Predictive Value of Tests; D055070:Resistance Training; D055815:Young Adult
chemical_list: D015415:Biomarkers; D035683:MicroRNAs
keywords: Anabolic resistance; miR-19b-3p; miR-206; miR-486