title: Analgesic effect of coumarins from Radix angelicae pubescentis is mediated by inflammatory factors and TRPV1 in a spared nerve injury model of neuropathic pain.
abstract: BACKGROUND
  Coumarins from Radix angelicae pubescentis (CRAP) are a major active component that are isolated from dried roots of Angelica biserrata Yuan et Shan, which has been used clinically to cure headaches for a long period of time, and it is an effective treatment for pain. The aim of the present study was to investigate the analgesic effect of CRAP on a spared nerve injury (SNI) model of neuropathy.
  
  
  METHODS
  Antinociceptive effects of CRAP were assessed in Sprague-Dawley male rats using a spared nerve injury model of neuropathic pain. Inflammatory factors were determined by Enzyme-linked immunosorbent assay (ELISA). Transient receptor potential cation channel 1 (TRPV1) and Phosphorylated extracellular regulated protein kinases (pERK) were detected by Immunofluorescence and Western blotting, respectively.
  
  
  RESULTS
  The high performance liquid chromatography (HPLC) analysis showed the presence of osthole and columbianadin in Radix angelicae pubescentis. CRAP induced the dose-dependent effect of on attenuating the development of mechanical hypersensitivity. Molecular profiling revealed that CRAP reduced the levels of pro-inflammatory cytokines tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β) and interleukin-6 (IL-6) and significantly attenuated the expression of TRPV1 and pERK in damaged DRG neurons.
  
  
  CONCLUSIONS
  This results demonstrate that CRAP possess remarkable antinociceptive activities which may be due to osthole and columbianadin at least in part, supporting the folkloric usage of the plant to treat various pain diseases.
authors.affiliation: Department of Pharmacy, Xijing Hospital, Fourth Military Medical University, Changle West Street 15, Xi'an, Shaanxi 710032, China.
authors.forename: Ruili
authors.initials: R
authors.lastname: Li
authors.affiliation: Department of Pharmacy, Xijing Hospital, Fourth Military Medical University, Changle West Street 15, Xi'an, Shaanxi 710032, China.
authors.forename: Chao
authors.initials: C
authors.lastname: Zhao
authors.affiliation: Department of Pharmacy, Xijing Hospital, Fourth Military Medical University, Changle West Street 15, Xi'an, Shaanxi 710032, China.
authors.forename: Minna
authors.initials: M
authors.lastname: Yao
authors.affiliation: Department of Psychiatry, Johns Hopkins University School of Medicine, Wolfe Street, Baltimore, MD 21287, USA.
authors.forename: Ying
authors.initials: Y
authors.lastname: Song
authors.affiliation: Department of Pharmacy, Xijing Hospital, Fourth Military Medical University, Changle West Street 15, Xi'an, Shaanxi 710032, China.
authors.forename: Yin
authors.initials: Y
authors.lastname: Wu
authors.affiliation: Department of Pharmacy, Xijing Hospital, Fourth Military Medical University, Changle West Street 15, Xi'an, Shaanxi 710032, China. Electronic address: lwrcll@163.com.
authors.forename: Aidong
authors.initials: A
authors.lastname: Wen
mesh_terms: D000700:Analgesics / Q000302:isolation & purification / Q000494:pharmacology*; D029969:Angelica / Q000737:chemistry*; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D003374:Coumarins / Q000302:isolation & purification / Q000494:pharmacology*; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism; D005727:Ganglia, Spinal / Q000187:drug effects* / Q000378:metabolism / Q000503:physiopathology; D018836:Inflammation Mediators / Q000378:metabolism*; D008297:Male; D009437:Neuralgia / Q000188:drug therapy* / Q000378:metabolism / Q000503:physiopathology; D017288:Pain Threshold / Q000187:drug effects; D010766:Phosphorylation; D008517:Phytotherapy; D018517:Plant Roots / Q000737:chemistry; D010946:Plants, Medicinal; D017207:Rats, Sprague-Dawley; D015398:Signal Transduction / Q000187:drug effects; D050916:TRPV Cation Channels / Q000187:drug effects* / Q000378:metabolism; D013997:Time Factors
chemical_list: D000700:Analgesics; D003374:Coumarins; D018836:Inflammation Mediators; D050916:TRPV Cation Channels; C493922:Trpv1 protein, rat; C064386:columbianadin; D048049:Extracellular Signal-Regulated MAP Kinases; C046627:osthol
keywords: Coumarin; Neuropathic pain; Radix angelicae pubescentis; TRPV1; pERK