title: Long Noncoding RNA Expression Signatures of Abdominal Aortic Aneurysm Revealed by Microarray.
abstract: OBJECTIVE
  This study is aimed at observing the role of long noncoding RNAs (lncRNAs) in the pathogenesis of abdominal aortic aneurysm (AAA).
  
  
  METHODS
  LncRNA and mRNA expression signatures of AAA tissues and normal abdominal aortic tissues (NT) were analyzed by microarray and further verified by Real-time quantitative reverse-transcription PCR (qRT-PCR). The lncRNAs-mRNAs targeting relationships were identified using computational analysis. The effect of lnc-ARG on 5-lipoxygenase (ALOX5) expression was tested in HeLa cells.
  
  
  RESULTS
  Differential expressions of 3,688 lncRNAs and 3,007 mRNAs were identified between AAA and NT tissues. Moreover, 1,284 differentially expressed long intergenic noncoding RNAs and 206 differentially expressed enhancer-like lncRNAs adjacent to protein-coding genes were discerned by bioinformatics analysis. Some differentially expressed lncRNAs and mRNAs between AAA and normal tissue samples were further verified using qRT-PCR. A co-expression network of coding and noncoding genes was constructed based on the correlation analysis between the differentially expressed lncRNAs and mRNAs. In addition, the lnc-ARG located within the upstream of ALOX5 was sorted as a noncoding transcript by analyzing the protein-coding potential using computational analysis. Furthermore, we found that lnc-ARG can decrease the mRNA level of ALOX5 and reactive oxygen species production in HeLa cells.
  
  
  CONCLUSIONS
  This study revealed new lncRNA candidates are related to the pathogenesis of AAA.
authors.affiliation: The Department of Vascular Surgery, Beijing Anzhen Hospital, Capital Medical University, Beijing 100029, China.
authors.forename: Yao Guo
authors.initials: YG
authors.lastname: Yang
authors.affiliation: State Key Laboratory of Brain and Cognitive Science, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China.
authors.forename: Mei Xia
authors.initials: MX
authors.lastname: Li
authors.affiliation: The Department of Vascular Surgery, Beijing Anzhen Hospital, Capital Medical University, Beijing 100029, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Kou
authors.affiliation: The Department of Vascular Surgery, Beijing Anzhen Hospital, Capital Medical University, Beijing 100029, China.
authors.forename: You
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: The Department of Vascular Surgery, Beijing Anzhen Hospital, Capital Medical University, Beijing 100029, China.
authors.forename: Yan Wen
authors.initials: YW
authors.lastname: Qin
authors.affiliation: State Key Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences Chinese Academy of Medical Sciences & School of Basic Medicine Peking Union Medical College, Beijing 100005, China.
authors.forename: Xiao Jun
authors.initials: XJ
authors.lastname: Liu
authors.affiliation: The Department of Vascular Surgery, Beijing Anzhen Hospital, Capital Medical University, Beijing 100029, China.
authors.forename: Zhong
authors.initials: Z
authors.lastname: Chen
mesh_terms: D000368:Aged; D017544:Aortic Aneurysm, Abdominal / Q000235:genetics* / Q000378:metabolism; D005260:Female; D020869:Gene Expression Profiling; D006801:Humans; D008297:Male; D008875:Middle Aged; D020411:Oligonucleotide Array Sequence Analysis; D062085:RNA, Long Noncoding / Q000235:genetics* / Q000378:metabolism
chemical_list: D062085:RNA, Long Noncoding
keywords: ALOX5; Abdominal aortic aneurysm; lnc-ARG; lncRNA