title: Cytogenetics for Biological Dosimetry.
abstract: Cytogenetics is the gold-standard in biological dosimetry for assessing a received dose of ionizing radiation. More modern techniques have recently emerged, but none are as specific as cytogenetic approaches, particularly the dicentric assay. Here, we will focus on the principal cytogenetic techniques used for biological dosimetry: the dicentric assay in metaphase cells, the micronuclei assay in binucleated cells, and the premature condensed chromosome (PCC) assay in interphase cells. New fluorescence in situ hybridization (FISH) techniques (such as telomere-centromere hybridization) have facilitated the analysis of the dicentric assay and have permitted to assess the dose a long time after irradiation by translocation analysis (such as by Tri-color FISH or Multiplex-FISH). Telomere centromere staining of PCCs will make it possible to perform dose assessment within 24 h of exposure in the near future.
authors.affiliation: PROCyTOX Commissariat à l'Energie Atomique et aux Energies Alternatives (CEA), Fontenay-aux-Roses and Université Paris-Saclay, 18 route du Panorama, 92265, Fontenay-aux-Roses cedex, France.
authors.forename: Michelle
authors.initials: M
authors.lastname: Ricoul
authors.affiliation: PROCyTOX Commissariat à l'Energie Atomique et aux Energies Alternatives (CEA), Fontenay-aux-Roses and Université Paris-Saclay, 18 route du Panorama, 92265, Fontenay-aux-Roses cedex, France.
authors.forename: Tamizh
authors.initials: T
authors.lastname: Gnana-Sekaran
authors.affiliation: PROCyTOX Commissariat à l'Energie Atomique et aux Energies Alternatives (CEA), Fontenay-aux-Roses and Université Paris-Saclay, 18 route du Panorama, 92265, Fontenay-aux-Roses cedex, France.
authors.forename: Laure
authors.initials: L
authors.lastname: Piqueret-Stephan
authors.affiliation: PROCyTOX Commissariat à l'Energie Atomique et aux Energies Alternatives (CEA), Fontenay-aux-Roses and Université Paris-Saclay, 18 route du Panorama, 92265, Fontenay-aux-Roses cedex, France. laure.sabatier@cea.fr.
authors.forename: Laure
authors.initials: L
authors.lastname: Sabatier
mesh_terms: D000818:Animals; D002869:Chromosome Aberrations / Q000528:radiation effects*; D020732:Cytogenetic Analysis / Q000379:methods*; D004307:Dose-Response Relationship, Radiation; D006801:Humans; D048629:Micronuclei, Chromosome-Defective; D008853:Microscopy; D011839:Radiation, Ionizing; D011874:Radiometry / Q000379:methods*; D014178:Translocation, Genetic
keywords: Biological dosimetry; Centromere; Chromosomal aberrations; Radiation effects; Telomere