title: Oral Cnm-positive Streptococcus Mutans Expressing Collagen Binding Activity is a Risk Factor for Cerebral Microbleeds and Cognitive Impairment.
abstract: Cerebral microbleeds (CMBs) are an important risk factor for stroke and dementia. We have shown that the collagen binding surface Cnm protein expressed on cnm-positive Streptococcus mutans is involved in the development of CMBs. However, whether the collagen binding activity of cnm-positive S. mutans is related to the nature of the CMBs or to cognitive impairment is unclear. Two-hundred seventy nine community residents (70.0 years) were examined for the presence or absence of cnm-positive S. mutans in the saliva by PCR and collagen binding activity, CMBs, and cognitive function were evaluated. Cnm-positive S. mutans was detected more often among subjects with CMBs (p < 0.01) than those without. The risk of CMBs was significantly higher (odds ratio = 14.3) in the group with S. mutans expressing collagen binding activity, as compared to the group without that finding. Deep CMBs were more frequent (67%) and cognitive function was lower among subjects with cnm-positive S. mutans expressing collagen binding activity. This work supports the role of oral health in stroke and dementia and proposes a molecular mechanism for the interaction.
authors.affiliation: Department of Epidemiology for Community health and Medicine, Kyoto Prefectural University of Medicine, Kyoto, 6028566, Japan.|Department of Dental Medicine, Kyoto Prefectural University of Medicine, Kyoto, 6028566, Japan.
authors.forename: Isao
authors.initials: I
authors.lastname: Watanabe
authors.affiliation: Department of Epidemiology for Community health and Medicine, Kyoto Prefectural University of Medicine, Kyoto, 6028566, Japan.|Department of Neurology, Kyoto Prefectural University of Medicine, Kyoto, 6028566, Japan.
authors.forename: Nagato
authors.initials: N
authors.lastname: Kuriyama
authors.affiliation: Department of Epidemiology for Community health and Medicine, Kyoto Prefectural University of Medicine, Kyoto, 6028566, Japan.|Department of Dental Medicine, Kyoto Prefectural University of Medicine, Kyoto, 6028566, Japan.
authors.forename: Fumitaro
authors.initials: F
authors.lastname: Miyatani
authors.affiliation: Department of Pediatric Dentistry, Osaka University Graduate School of Dentistry, Osaka, 5650871, Japan.
authors.forename: Ryota
authors.initials: R
authors.lastname: Nomura
authors.affiliation: Department of Pediatric Dentistry, Osaka University Graduate School of Dentistry, Osaka, 5650871, Japan.
authors.forename: Shuhei
authors.initials: S
authors.lastname: Naka
authors.affiliation: Department of Pediatric Dentistry, Osaka University Graduate School of Dentistry, Osaka, 5650871, Japan.
authors.forename: Kazuhiko
authors.initials: K
authors.lastname: Nakano
authors.affiliation: Department of Stroke and Cerebrovascular Diseases, National Cerebral and Cardiovascular Center, Osaka, 5658565, Japan.
authors.forename: Masafumi
authors.initials: M
authors.lastname: Ihara
authors.affiliation: Department of Epidemiology for Community health and Medicine, Kyoto Prefectural University of Medicine, Kyoto, 6028566, Japan.|Department of Dental Medicine, Kyoto Prefectural University of Medicine, Kyoto, 6028566, Japan.
authors.forename: Komei
authors.initials: K
authors.lastname: Iwai
authors.affiliation: Department of Epidemiology for Community health and Medicine, Kyoto Prefectural University of Medicine, Kyoto, 6028566, Japan.|Department of Dental Medicine, Kyoto Prefectural University of Medicine, Kyoto, 6028566, Japan.
authors.forename: Daisuke
authors.initials: D
authors.lastname: Matsui
authors.affiliation: Department of Epidemiology for Community health and Medicine, Kyoto Prefectural University of Medicine, Kyoto, 6028566, Japan.
authors.forename: Etsuko
authors.initials: E
authors.lastname: Ozaki
authors.affiliation: Department of Epidemiology for Community health and Medicine, Kyoto Prefectural University of Medicine, Kyoto, 6028566, Japan.
authors.forename: Teruhide
authors.initials: T
authors.lastname: Koyama
authors.affiliation: Department of Dental Medicine, Kyoto Prefectural University of Medicine, Kyoto, 6028566, Japan.
authors.forename: Masaru
authors.initials: M
authors.lastname: Nishigaki
authors.affiliation: Department of Dental Medicine, Kyoto Prefectural University of Medicine, Kyoto, 6028566, Japan.
authors.forename: Toshiro
authors.initials: T
authors.lastname: Yamamoto
authors.affiliation: Department of Neurology, Kyoto Prefectural University of Medicine, Kyoto, 6028566, Japan.
authors.forename: Aiko
authors.initials: A
authors.lastname: Tamura
authors.affiliation: Department of Neurology, Kyoto Prefectural University of Medicine, Kyoto, 6028566, Japan.
authors.forename: Toshiki
authors.initials: T
authors.lastname: Mizuno
authors.affiliation: Department of Radiology, Kyoto Prefectural University of Medicine, Kyoto, 6028566, Japan.
authors.forename: Kentaro
authors.initials: K
authors.lastname: Akazawa
authors.affiliation: Kyoto Industrial Health Association, Kyoto, 6048472, Japan.
authors.forename: Akihiro
authors.initials: A
authors.lastname: Takada
authors.affiliation: Kyoto Industrial Health Association, Kyoto, 6048472, Japan.
authors.forename: Kazuo
authors.initials: K
authors.lastname: Takeda
authors.affiliation: Department of Radiology, Kyoto Prefectural University of Medicine, Kyoto, 6028566, Japan.
authors.forename: Kei
authors.initials: K
authors.lastname: Yamada
authors.affiliation: Department of Neurology, Kyoto Prefectural University of Medicine, Kyoto, 6028566, Japan.|North Medical Center, Kyoto Prefectural University of Medicine, Kyoto, 6292261, Japan.
authors.forename: Masanori
authors.initials: M
authors.lastname: Nakagawa
authors.affiliation: Department of Neurosurgery, Seirei Hamamatsu General Hospital, Shizuoka, 4308558, Japan.
authors.forename: Tokutaro
authors.initials: T
authors.lastname: Tanaka
authors.affiliation: Department of Dental Medicine, Kyoto Prefectural University of Medicine, Kyoto, 6028566, Japan.
authors.forename: Narisato
authors.initials: N
authors.lastname: Kanamura
authors.affiliation: Department of Neurology, University of Louisville, KY, 40202, USA.
authors.forename: Robert P
authors.initials: RP
authors.lastname: Friedland
authors.affiliation: Department of Epidemiology for Community health and Medicine, Kyoto Prefectural University of Medicine, Kyoto, 6028566, Japan.
authors.forename: Yoshiyuki
authors.initials: Y
authors.lastname: Watanabe
mesh_terms: D018829:Adhesins, Bacterial / Q000378:metabolism*; D000368:Aged; D002352:Carrier Proteins / Q000378:metabolism*; D002543:Cerebral Hemorrhage / Q000150:complications* / Q000382:microbiology*; D060825:Cognitive Dysfunction / Q000150:complications* / Q000382:microbiology*; D003094:Collagen / Q000378:metabolism*; D005260:Female; D006801:Humans; D008279:Magnetic Resonance Imaging; D008297:Male; D009055:Mouth / Q000382:microbiology*; D016017:Odds Ratio; D011485:Protein Binding; D012307:Risk Factors; D013295:Streptococcus mutans / Q000378:metabolism*
chemical_list: D018829:Adhesins, Bacterial; D002352:Carrier Proteins; C488758:Cnm protein, Streptococcus mutans; D003094:Collagen