title: Improved rhBMP-2 function on MBG incorporated TiO2nanorod films.
abstract: In the process of biomaterials mediated bone regeneration, rhBMP-2 delivery at efficient dose in sustained kinetics is crucial for promoting cell osteogenic differentiation. Meanwhile, surface morphology of the biomaterials could regulate cellular responses as well as strengthen the rhBMP-2 interaction with cells for better bone induction. Herein, TiO2nanorod films with varied mesoporous bioactive glass (MBG) incorporation amount were designed to strengthen the efficacy of rhBMP-2, basing on optimized loading/release behaviors and surface nanostructure cooperatively. The MBG incorporation improved rhBMP-2 loading amount and regulated its release behavior. Consequently, the osteogenic differentiation of bone marrow-derived mesenchymal stem cells (BMSCs) on the incorporated films was extremely enhanced, and the incorporated nanorod film with 200nm MBG thickness exhibited the best osteoinduction effect. However, MBG film and the incorporated nanorod film had the same loading amount of rhBMP-2, the latter showed a much higher expression of 7-day osteogenic differentiation index than the former, which could be attributed to the synergistic effect of optimized rhBMP-2 release behavior and surface morphology. The MBG incorporated TiO2nanorod films here presents a promising strategy for enhancing osteoinduction through optimized rhBMP-2 release behavior.
authors.affiliation: School of Materials Science and Engineering, State Key Laboratory of Silicon Materials, Zhejiang University, Hangzhou 310027, China.
authors.forename: Fei
authors.initials: F
authors.lastname: Ge
authors.affiliation: The First Affiliated Hospital of Medical College, Zhejiang University, Hangzhou 310003, China.
authors.forename: Mengfei
authors.initials: M
authors.lastname: Yu
authors.affiliation: School of Materials Science and Engineering, State Key Laboratory of Silicon Materials, Zhejiang University, Hangzhou 310027, China.
authors.forename: Cuixia
authors.initials: C
authors.lastname: Yu
authors.affiliation: The First Affiliated Hospital of Medical College, Zhejiang University, Hangzhou 310003, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Lin
authors.affiliation: School of Materials Science and Engineering, State Key Laboratory of Silicon Materials, Zhejiang University, Hangzhou 310027, China. Electronic address: wengwj@zju.edu.
authors.forename: Wenjian
authors.initials: W
authors.lastname: Weng
authors.affiliation: School of Materials Science and Engineering, State Key Laboratory of Silicon Materials, Zhejiang University, Hangzhou 310027, China.
authors.forename: Kui
authors.initials: K
authors.lastname: Cheng
authors.affiliation: The First Affiliated Hospital of Medical College, Zhejiang University, Hangzhou 310003, China. Electronic address: wangmysm@yahoo.com.cn.
authors.forename: Huiming
authors.initials: H
authors.lastname: Wang
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry; D001854:Bone Marrow Cells / Q000166:cytology; D055396:Bone Morphogenetic Protein 2 / Q000737:chemistry*; D001861:Bone Regeneration / Q000187:drug effects; D001842:Bone and Bones / Q000378:metabolism; D002454:Cell Differentiation; D005898:Glass / Q000737:chemistry*; D006801:Humans; D007700:Kinetics; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D053768:Metal Nanoparticles / Q000737:chemistry; D008855:Microscopy, Electron, Scanning; D043942:Nanotubes / Q000737:chemistry*; D010012:Osteogenesis; D051381:Rats; D017207:Rats, Sprague-Dawley; D060888:Real-Time Polymerase Chain Reaction; D011994:Recombinant Proteins / Q000737:chemistry*; D054457:Tissue Scaffolds / Q000737:chemistry; D014025:Titanium / Q000737:chemistry*
chemical_list: C525718:BMP2 protein, human; D001672:Biocompatible Materials; D055396:Bone Morphogenetic Protein 2; D011994:Recombinant Proteins; C009495:titanium dioxide; D014025:Titanium
keywords: BMSCs; Mesoporous bioactive glass; Nanorods; rhBMP-2