title: Engineering of Escherichia coli to facilitate efficient utilization of isomaltose and panose in industrial glucose feedstock.
abstract: Industrial glucose feedstock prepared by enzymatic digestion of starch typically contains significant amounts of disaccharides such as maltose and isomaltose and trisaccharides such as maltotriose and panose. Maltose and maltosaccharides can be utilized in Escherichia coli fermentation using industrial glucose feedstock because there is an intrinsic assimilation pathway for these sugars. However, saccharides that contain α-1,6 bonds, such as isomaltose and panose, are still present after fermentation because there is no metabolic pathway for these sugars. To facilitate more efficient utilization of glucose feedstock, we introduced glvA, which encodes phospho-α-glucosidase, and glvC, which encodes a subunit of the phosphoenolpyruvate-dependent maltose phosphotransferase system (PTS) of Bacillus subtilis, into E. coli. The heterologous expression of glvA and glvC conferred upon the recombinant the ability to assimilate isomaltose and panose. The recombinant E. coli assimilated not only other disaccharides but also trisaccharides, including alcohol forms of these saccharides, such as isomaltitol. To the best of our knowledge, this is the first report to show the involvement of the microbial PTS in the assimilation of trisaccharides. Furthermore, we demonstrated that an L-lysine-producing E. coli harboring glvA and glvC converted isomaltose and panose to L-lysine efficiently. These findings are expected to be beneficial for industrial fermentation.
authors.affiliation: Process Development Laboratories, Research Institute for Bioscience Products & Fine Chemicals, Ajinomoto Co., Inc., 1-1 Suzuki-cho, Kawasaki-ku, Kawasaki-shi, Kanagawa, 210-8681, Japan. kenji_abe68@ajinomoto.com.|Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University, 1-3-1 Kagamiyama, Higashi-Hiroshima-shi, Hiroshima, 739-8530, Japan. kenji_abe68@ajinomoto.com.
authors.forename: Kenji
authors.initials: K
authors.lastname: Abe
authors.affiliation: Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University, 1-3-1 Kagamiyama, Higashi-Hiroshima-shi, Hiroshima, 739-8530, Japan.
authors.forename: Akio
authors.initials: A
authors.lastname: Kuroda
authors.affiliation: Process Development Laboratories, Research Institute for Bioscience Products & Fine Chemicals, Ajinomoto Co., Inc., 1-1 Suzuki-cho, Kawasaki-ku, Kawasaki-shi, Kanagawa, 210-8681, Japan.
authors.forename: Ryo
authors.initials: R
authors.lastname: Takeshita
mesh_terms: D000821:Animal Feed; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism*; D005936:Glucans / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism; D005964:Glucosyltransferases / Q000235:genetics* / Q000378:metabolism; D007534:Isomaltose / Q000378:metabolism*; D008239:Lysine / Q000378:metabolism; D008320:Maltose / Q000378:metabolism; D013402:Sugar Alcohols / Q000378:metabolism; D000520:alpha-Glucosidases / Q000235:genetics* / Q000378:metabolism
chemical_list: D005936:Glucans; D013402:Sugar Alcohols; C008763:panose; D007534:Isomaltose; D008320:Maltose; D005964:Glucosyltransferases; C021535:maltose phosphorylase; D000520:alpha-Glucosidases; D005947:Glucose; D008239:Lysine; C008187:isomaltitol
keywords: Assimilation; Bacillus subtilis; Escherichia coli; Isomaltose; L-Lysine production; Maltose; Panose; glvA; glvC