title: Podoplanin Expression in Canine Melanoma.
abstract: A type I transmembrane protein, podoplanin (PDPN), is expressed in several normal cells such as lymphatic endothelial cells or pulmonary type I alveolar cells. We recently demonstrated that anticanine PDPN monoclonal antibody (mAb), PMab-38, recognizes canine PDPN of squamous cell carcinomas, but does not react with lymphatic endothelial cells. Herein, we investigated whether PMab-38 reacts with canine melanoma. PMab-38 reacted with 90% of melanoma cells (9/10 cases) using immunohistochemistry. Of interest, PMab-38 stained the lymphatic endothelial cells and cancer-associated fibroblasts in melanoma tissues, although it did not stain any lymphatic endothelial cells in normal tissues. PMab-38 could be useful for uncovering the function of PDPN in canine melanomas.
authors.affiliation: 1 Department of Regional Innovation, Tohoku University Graduate School of Medicine , Miyagi, Japan .
authors.forename: Satoshi
authors.initials: S
authors.lastname: Ogasawara
authors.affiliation: 1 Department of Regional Innovation, Tohoku University Graduate School of Medicine , Miyagi, Japan .
authors.forename: Ryusuke
authors.initials: R
authors.lastname: Honma
authors.affiliation: 1 Department of Regional Innovation, Tohoku University Graduate School of Medicine , Miyagi, Japan .
authors.forename: Mika K
authors.initials: MK
authors.lastname: Kaneko
authors.affiliation: 1 Department of Regional Innovation, Tohoku University Graduate School of Medicine , Miyagi, Japan .
authors.forename: Yuki
authors.initials: Y
authors.lastname: Fujii
authors.affiliation: 2 North Lab , Hokkaido, Japan .
authors.forename: Yumiko
authors.initials: Y
authors.lastname: Kagawa
authors.affiliation: 3 Department of Disease Control, Graduate School of Veterinary Medicine, Hokkaido University , Hokkaido, Japan .
authors.forename: Satoru
authors.initials: S
authors.lastname: Konnai
authors.affiliation: 1 Department of Regional Innovation, Tohoku University Graduate School of Medicine , Miyagi, Japan .
authors.forename: Yukinari
authors.initials: Y
authors.lastname: Kato
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000737:chemistry*; D000918:Antibody Specificity; D000072645:Cancer-Associated Fibroblasts / Q000276:immunology / Q000473:pathology; D004283:Dog Diseases / Q000175:diagnosis* / Q000235:genetics / Q000276:immunology / Q000473:pathology; D004285:Dogs; D042783:Endothelial Cells / Q000276:immunology / Q000473:pathology*; D015870:Gene Expression; D007150:Immunohistochemistry; D008545:Melanoma / Q000175:diagnosis / Q000235:genetics / Q000276:immunology / Q000662:veterinary*; D008562:Membrane Glycoproteins / Q000032:analysis / Q000235:genetics* / Q000276:immunology; D009062:Mouth Neoplasms / Q000175:diagnosis / Q000235:genetics / Q000276:immunology / Q000662:veterinary*
chemical_list: D000911:Antibodies, Monoclonal; D008562:Membrane Glycoproteins
keywords: canine podoplanin; immunohistochemistry; melanoma; monoclonal antibody