title: Aneurinifactin, a new lipopeptide biosurfactant produced by a marine Aneurinibacillus aneurinilyticus SBP-11 isolated from Gulf of Mannar: Purification, characterization and its biological evaluation.
abstract: Biosurfactants are microbial-derived amphiphilic molecules having hydrophobic and hydrophilic moieties produced by bacteria, fungi, yeasts and algae and are extracellular or cell wall-associated compounds. In an ongoing survey for bioactive microbial metabolites from microbes isolated from diverse ecological niches, a new lipopeptide biosurfactant was identified from a marine bacterium; Aneurinibacillus aneurinilyticus strain SBP-11, which was isolated from a marine diversity hotspot, Gulf of Mannar, India. A new lipopeptide biosurfactant was purified and characterized based on TLC, FT-IR, NMR, GC-MS, HPLC, MALDI-TOF-MS and tandem MS analysis as Stearic acid-Thr-Tyr-Val-Ser-Tyr-Thr (named as Aneurinifactin). The critical micelle concentration of Aneurinifactin was 26mgL-1at a surface tension of 26mNm-1. Further, the biosurfactant showed stable emulsification at a wide range of pH (2-9) and temperature up to 80°C. Aneurinifactin showed promising antimicrobial activity and concentration dependent efficient oil recovery. This is the first report on Aneurinifactin, a lipopeptide biosurfactant produced by a marine A. aneurinilyticus SBP-11, which could be explored as a promising candidate for use in various biomedical and industrial applications.
authors.affiliation: CAS in Marine Biology, Faculty of Marine Sciences, Annamalai University, Porto Novo, Tamil Nadu 608502, India; Present address: Department of Medicinal Plant Biotechnology, Sharmila Institute of Medicinal Products Research Academy, Thanjavur, Tamil Nadu 613007, India.
authors.forename: Shanmugasundaram Senthil
authors.initials: SS
authors.lastname: Balan
authors.affiliation: Medicinal Chemistry and Pharmacology Division, CSIR-Indian Institute of Chemical Technology, Tarnaka, Hyderabad 500007, India. Electronic address: cgkumar@iict.res.in.
authors.forename: C Ganesh
authors.initials: CG
authors.lastname: Kumar
authors.affiliation: CAS in Marine Biology, Faculty of Marine Sciences, Annamalai University, Porto Novo, Tamil Nadu 608502, India.
authors.forename: Singaram
authors.initials: S
authors.lastname: Jayalakshmi
mesh_terms: D000890:Anti-Infective Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D056449:Bacillales / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D002855:Chromatography, Thin Layer / Q000379:methods; D045703:Emulsifying Agents / Q000737:chemistry; D008401:Gas Chromatography-Mass Spectrometry; D055666:Lipopeptides / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D008826:Microbial Sensitivity Tests / Q000379:methods; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods; D013501:Surface-Active Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*
chemical_list: D000890:Anti-Infective Agents; D045703:Emulsifying Agents; D055666:Lipopeptides; D013501:Surface-Active Agents
keywords: Aneurinibacillus aneurinilyticus; Antimicrobial; Biosurfactant; Emulsification; Lipopeptide; Oil recovery