title: Olive polyphenol effects in a mouse model of chronic ethanol addiction.
abstract: OBJECTIVE
  Alcohol addiction elicits oxidative imbalance and it is well known that polyphenols possess antioxidant properties. We investigated whether or not polyphenols could confer a protective potential against alcohol-induced oxidative stress.
  
  
  METHODS
  We administered (per os) for two months 20 mg/kg of olive polyphenols containing mostly hydroxytyrosol in alcoholic adult male mice. Hydroxytyrosol metabolites as hydroxytyrosol sulfate 1 and hydroxytyrosol sulfate 2 were found in the serum of mice administered with polyphenols with the highest amount in animals treated with both polyphenols and alcohol. Oxidative stress was evaluated by FORT (free oxygen radical test) and FORD (free oxygen radical defense) tests.
  
  
  RESULTS
  Alcoholic mice showed a worse oxidative status than nonalcoholic mice (higher FORT and lower FORD) but polyphenol supplementation partially counteracted the alcohol pro-oxidant effects, as evidenced by FORT.
  
  
  CONCLUSIONS
  A better understanding of the antioxidant protection provided by polyphenols might be of primary interest for drug discovery and dietary-based prevention of the damage associated with chronic alcohol abuse.
authors.affiliation: Institute of Cell Biology and Neurobiology, CNR; IRCCS S. Lucia Foundation, Italy.
authors.forename: Valentina
authors.initials: V
authors.lastname: Carito
authors.affiliation: CRARL Lazio, Sapienza University, Rome, Italy.
authors.forename: Mauro
authors.initials: M
authors.lastname: Ceccanti
authors.affiliation: Institute of Cell Biology and Neurobiology, CNR; IRCCS S. Lucia Foundation, Italy.
authors.forename: Vincenzo
authors.initials: V
authors.lastname: Cestari
authors.affiliation: Entecra, Rome, Italy.
authors.forename: Fausta
authors.initials: F
authors.lastname: Natella
authors.affiliation: Entecra, Rome, Italy.
authors.forename: Cristiano
authors.initials: C
authors.lastname: Bello
authors.affiliation: Institute of Cell Biology and Neurobiology, CNR; IRCCS S. Lucia Foundation, Italy.
authors.forename: Roberto
authors.initials: R
authors.lastname: Coccurello
authors.affiliation: Centro Nazionale Sostanze Chimiche, ISS, Rome, Italy.
authors.forename: Rosanna
authors.initials: R
authors.lastname: Mancinelli
authors.affiliation: Institute of Cell Biology and Neurobiology, CNR; IRCCS S. Lucia Foundation, Italy. Electronic address: marco.fiore@cnr.it.
authors.forename: Marco
authors.initials: M
authors.lastname: Fiore
mesh_terms: D000437:Alcoholism / Q000378:metabolism*; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D016739:Behavior, Addictive; D002908:Chronic Disease; D019587:Dietary Supplements; D000431:Ethanol / Q000009:adverse effects*; D005609:Free Radicals; D008297:Male; D051379:Mice; D031658:Olea / Q000737:chemistry*; D010084:Oxidation-Reduction; D018384:Oxidative Stress / Q000187:drug effects*; D010936:Plant Extracts / Q000494:pharmacology*; D059808:Polyphenols / Q000494:pharmacology*
chemical_list: D000975:Antioxidants; D005609:Free Radicals; D010936:Plant Extracts; D059808:Polyphenols; D000431:Ethanol
keywords: Alcoholism; Mouse; Olive polyphenols; Oxidative stress