title: Intrauterine administration of hCG-activated autologous human peripheral blood mononuclear cells (PBMC) promotes live birth rates in frozen/thawed embryo transfer cycles of patients with repeated implantation failure.
abstract: Recurrent implantation failure refers to unsuccessful implantation after repeated transfers of morphologically good quality embryos into a normal uterus. Recently, accumulating evidence has suggested that local immune cells at the implantation site have actively contributed to embryo implantation. Our aim was to study the effects of intrauterine administration of hCG-activated autologous human PBMC on clinical pregnancy, implantation rates and live birth rate of patients who received frozen/thawed embryo transfer. We observed patients with one to three failed transplantations cannot benefit from the administration, but the rate of clinical pregnancy (39.58% vs. 14.29%), live birth (33.33% vs. 9.58%) and implantation (22.00% vs. 4.88%) were significantly increased in patients with four or more failures, respectively. For patients with endometrial thickness more than 7mm and less than 8mm on day of embryo transfer, the implantation rate (22.69% vs. 14.21%) and the live birth rate significantly higher in the PBMC-treated group; For patients who had RIF and received frozen/thawed early cleavage stage embryo transfer, the live birth delivery rate (29.63% vs. 13.33%) significant higher in PBMC-treated group. These findings indicate that intrauterine administration of hCG-activated autologous PBMC effectively improves the IVF outcomes for RIF patients, especially for the RIF patients with cleavage stage embryo transfer, patients with thin endometrial thickness also benefit from this approach.
authors.affiliation: Reproductive Medicine Center, Renmin Hospital of Wuhan University, Wuhan, PR China.
authors.forename: Saijiao
authors.initials: S
authors.lastname: Li
authors.affiliation: Reproductive Medicine Center, Renmin Hospital of Wuhan University, Wuhan, PR China.
authors.forename: Jing
authors.initials: J
authors.lastname: Wang
authors.affiliation: Reproductive Medicine Center, Renmin Hospital of Wuhan University, Wuhan, PR China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Cheng
authors.affiliation: Reproductive Medicine Center, Renmin Hospital of Wuhan University, Wuhan, PR China.
authors.forename: Danni
authors.initials: D
authors.lastname: Zhou
authors.affiliation: Reproductive Medicine Center, Renmin Hospital of Wuhan University, Wuhan, PR China.
authors.forename: Tailang
authors.initials: T
authors.lastname: Yin
authors.affiliation: Reproductive Medicine Center, Renmin Hospital of Wuhan University, Wuhan, PR China.
authors.forename: Wangming
authors.initials: W
authors.lastname: Xu
authors.affiliation: Department of Obstetrics and Gynecology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, PR China.
authors.forename: Nan
authors.initials: N
authors.lastname: Yu
authors.affiliation: Reproductive Medicine Center, Renmin Hospital of Wuhan University, Wuhan, PR China. Electronic address: Dryangqing@hotmail.com.
authors.forename: Jing
authors.initials: J
authors.lastname: Yang
mesh_terms: D000026:Abortion, Habitual / Q000628:therapy*; D000328:Adult; D001804:Blood Transfusion, Autologous; D001805:Blood Transfusion, Intrauterine; D002478:Cells, Cultured; D006063:Chorionic Gonadotropin / Q000276:immunology; D015925:Cryopreservation; D010064:Embryo Implantation; D004624:Embryo Transfer; D004717:Endometrium / Q000473:pathology*; D005260:Female; D005307:Fertilization in Vitro; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000276:immunology* / Q000637:transplantation; D008213:Lymphocyte Activation; D011247:Pregnancy; D018873:Pregnancy Rate; D016896:Treatment Outcome
chemical_list: D006063:Chorionic Gonadotropin
keywords: Frozen/thawed embryo transfer; HCG-activated autologous human peripheral blood mononuclear cells (PBMC); Repeated implantation failure