title: Effect of ulinastatin on the permeability of the blood-brain barrier on rats with global cerebral ischemia/reperfusion injury as assessed by MRI.
abstract: The study was designed to evaluate the effect of ulinastatin on the permeability of the blood-brain barrier in rats with global cerebral ischemia/reperfusion injury using MRI. A total of 108 Wistar rats (240 g-280g) were randomly divided into three groups (n=36): sham group (S group), global cerebral ischemia/reperfusion model group (GCI/R group) and 10,000U/kg ulinastatin intervention group (U group). Fifty-four Wistar rats were used for MRI, and the rest were used for Evans Blue(EB)analysis. We used the Pulsinelli four-vessel occlusion (4-VO) model of global cerebral ischemia/reperfusion to investigate the integrity of the blood-brain barrier with Evans Blue (EB) staining at 15min after ischemia and at 6h (n=6), 24h (n=6), and 48h (n=6) after reperfusion to assess blood-brain barrier permeability with MRI. In the ulinastatin treatment group, the area of EB staining was significantly smaller, the exudation of EB decreased significantly after cerebral ischemia/reperfusion at 6h, 24h, 48h, compared to the model group at corresponding time points (P<0.05) but increased compared to the sham group. The model group exhibited significantly highlighted regions of Gd-DTPA after post-contrast at the corresponding areas and time points compared with the sham group (P<0.05). The highlighted regions of Gd-DTPA in the ulinastatin treatment group were significantly higher compared with those of the sham group but lower when compared with those of the model group (P<0.05). The integrity of the blood-brain barrier after cerebral ischemia/reperfusion injury was damaged. Ulinastatin could significantly improve the permeability of the blood-brain barrier after cerebral ischemia/reperfusion injury in rats.
authors.affiliation: Department of Respiration, Shanghai Punan Hospital, Shanghai, PR China; Emergency Department, Jinshan Hospital of Fudan University of Shanghai, Shanghai, PR China.
authors.forename: Ming
authors.initials: M
authors.lastname: Liu
authors.affiliation: Emergency Department, Jinshan Hospital of Fudan University of Shanghai, Shanghai, PR China. Electronic address: 1696184155@qq.com.
authors.forename: Jie
authors.initials: J
authors.lastname: Shen
authors.affiliation: Master, Shanghai Pudong Gongli Hospital, Shanghai, PR China.
authors.forename: Fan
authors.initials: F
authors.lastname: Zou
authors.affiliation: Department of Respiration, Shanghai Punan Hospital, Shanghai, PR China.
authors.forename: Yunfeng
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: Laboratory Department, Jinshan Hospital of Fudan University of Shanghai, PR China.
authors.forename: Bing
authors.initials: B
authors.lastname: Li
authors.affiliation: Department of Physics of East China Normal University, Laboratory of Shanghai, Shanghai, PR China.
authors.forename: Mingxia
authors.initials: M
authors.lastname: Fan
mesh_terms: D000818:Animals; D001812:Blood-Brain Barrier / Q000187:drug effects*; D002545:Brain Ischemia / Q000000981:diagnostic imaging / Q000473:pathology*; D006023:Glycoproteins / Q000494:pharmacology*; D008279:Magnetic Resonance Imaging; D051381:Rats; D015427:Reperfusion Injury / Q000000981:diagnostic imaging / Q000473:pathology*; D014361:Trypsin Inhibitors / Q000494:pharmacology
chemical_list: D006023:Glycoproteins; D014361:Trypsin Inhibitors; C028665:urinastatin
keywords: Global cerebral ischemia/reperfusion injury; MRI; Ulinastatin