title: Molecular Phylogeny and Taxonomy of a New Freshwater Hymenostomatid from Northeastern China, with the Establishment of a New Genus Anteglaucoma gen. n. (Protista, Ciliophora, Oligohymenophorea).
abstract: The morphology, infraciliature and SSU rDNA sequence of a new freshwater hymenostomatid ciliate, Anteglaucoma harbinensis gen. nov., spec. nov., collected from a farmland pond in Harbin, China, were investigated. The new genus Anteglaucoma is characterized as follows: small to medium-sized Glaucomidae with oral apparatus in anterior one-third of cell; paroral membrane composed of almost longitudinally arranged dikinetids; three adoral membranelles nearly equal in length and arranged almost longitudinally in parallel; silverline pattern tetrahymenid. The improved diagnosis of family Glaucomidae Corliss 1971 is provided based on the previous and present work. The type species Anteglaucoma harbinensis spec. nov. is defined by having 32-35 somatic kineties; four or five postoral kineties; membranelle 1 and membranelle 2 having five or six kinetosomal rows, membranelle 3 having three kinetosomal rows; single macronuclear nodule; contractile vacuole on average 15% from posterior body end; locomotion characterized by crawling with a rather hectic jerking motion; freshwater habitat. Phylogenetic analyses show that Anteglaucoma clusters in the family Glaucomidae and groups with the genera Glaucoma. The molecular and morphological data indicate that Glaucomidae is related to the family Bromeliophryidae in the phylogenetic trees.
authors.affiliation: College of Life Science and Technology, Harbin Normal University, Harbin, 150025, China.
authors.forename: Xuming
authors.initials: X
authors.lastname: Pan
authors.affiliation: College of Life Science and Technology, Harbin Normal University, Harbin, 150025, China.
authors.forename: Zihan
authors.initials: Z
authors.lastname: Shi
authors.affiliation: Institute of Evolution & Marine Biodiversity, Ocean University of China, Qingdao, 266003, China.
authors.forename: Chundi
authors.initials: C
authors.lastname: Wang
authors.affiliation: Department of Biological Sciences, Boise State University, Boise, Idaho, 83725-1515.
authors.forename: William A
authors.initials: WA
authors.lastname: Bourland
authors.affiliation: College of Life Science and Technology, Harbin Normal University, Harbin, 150025, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Institute of Evolution & Marine Biodiversity, Ocean University of China, Qingdao, 266003, China.
authors.forename: Weibo
authors.initials: W
authors.lastname: Song
mesh_terms: D002681:China; D016054:DNA, Protozoan / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D005618:Fresh Water / Q000469:parasitology*; D016803:Hymenostomatida / Q000145:classification* / Q000235:genetics / Q000648:ultrastructure; D010802:Phylogeny; D017422:Sequence Analysis, DNA / Q000379:methods*
chemical_list: D016054:DNA, Protozoan; D004275:DNA, Ribosomal
keywords: Anteglaucoma harbinensis spec. nov.; Glaucomidae; new species