title: Exploiting Surface-Plasmon-Enhanced Light Scattering for the Design of Ultrasensitive Biosensing Modality.
abstract: Development of new detection methodologies and amplification schemes is indispensable for plasmonic biosensors to improve the sensitivity for the detection of trace amounts of analytes. Herein, an ultrasensitive scheme for signal enhancement based on the concept of surface-plasmon-resonance-enhanced light scattering (SP-LS) was validated experimentally and theoretically. The SP-LS of gold nanoparticles' (AuNPs) tags was employed in a sandwich assay for the detection of cardiac troponin I and provided up to 2 orders of magnitude improved sensitivity over conventional AuNPs-enhanced refractometric measurements and 3 orders of magnitude improvement over label-free SPR. Simulations were also performed to provide insights into the physical mechanisms.
authors.affiliation: Future Industries Institute, University of South Australia , Mawson Lakes Campus, Mawson Lakes, South Australia 5095, Australia.
authors.forename: Chih-Tsung
authors.initials: CT
authors.lastname: Yang
authors.affiliation: Electronics and Photonics Department, Institute of High Performance Computing, Agency for Science, Technology, and Research (A*STAR) , Singapore 138632.
authors.forename: Lin
authors.initials: L
authors.lastname: Wu
authors.affiliation: Center for Biomimetic Sensor Science, School of Materials Science and Engineering, Nanyang Technological University , Singapore 639798.
authors.forename: Xiaohu
authors.initials: X
authors.lastname: Liu
authors.affiliation: Center for Biomimetic Sensor Science, School of Materials Science and Engineering, Nanyang Technological University , Singapore 639798.
authors.forename: Nhung Thi
authors.initials: NT
authors.lastname: Tran
authors.affiliation: Electronics and Photonics Department, Institute of High Performance Computing, Agency for Science, Technology, and Research (A*STAR) , Singapore 138632.
authors.forename: Ping
authors.initials: P
authors.lastname: Bai
authors.affiliation: Center for Biomimetic Sensor Science, School of Materials Science and Engineering, Nanyang Technological University , Singapore 639798.
authors.forename: Bo
authors.initials: B
authors.lastname: Liedberg
authors.affiliation: Center for Biomimetic Sensor Science, School of Materials Science and Engineering, Nanyang Technological University , Singapore 639798.|Wenzhou Institute of Biomaterials and Engineering, Chinese Academy of Sciences , Wenzhou, Zhejiang 325001, China.|Institute of Biomaterials and Engineering, Wenzhou Medical University , Wenzhou, Zhejiang 325035, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Future Industries Institute, University of South Australia , Mawson Lakes Campus, Mawson Lakes, South Australia 5095, Australia.
authors.forename: Benjamin
authors.initials: B
authors.lastname: Thierry
mesh_terms: D004867:Equipment Design; D006801:Humans; D008027:Light; D012542:Scattering, Radiation; D020349:Surface Plasmon Resonance / Q000295:instrumentation / Q000379:methods*; D019210:Troponin I / Q000032:analysis*
chemical_list: D019210:Troponin I