title: Investigation for role of tissue factor and blood coagulation system in severe acute pancreatitis and associated liver injury.
abstract: This study aims to investigate the molecular mechanisms underlying the pathogenesis of severe acute pancreatitis (SAP) and SAP-associated liver injury, we performed an association analysis of the functions of tissue factor (TF) and blood coagulation system in both SAP patients and mouse SAP model. Our results showed that serum TF and tissue factor-microparticle (TF-MP) levels were highly up-regulated in both SAP patients and SAP mouse model, which was accompanied by the dysfunction of blood coagulation system. Besides, TF expression was also highly up-regulated in the Kupffer cells (KCs) of SAP mouse model. After inhibiting KCs in SAP mouse model, the amelioration of blood coagulation system functions was associated with the decrease in serum TF and TF-MPs levels, and the reduction of SAP-associated liver injury was associated with the decrease of TF expression in KCs. In conclusion, the dis-regulated TF expression and associated dysfunction of blood coagulation system are critical factors for the pathogenesis of SAP and SAP-associated liver injury. TF may serve as a potential and effective target for treating SAP and SAP-associated liver injury.
authors.affiliation: Department of Hepatobiliary Surgery, The Second Affiliated Hospital of Chongqing Medical University, Chongqing, People's Republic of China.
authors.forename: Zhi-Bing
authors.initials: ZB
authors.lastname: Ou
authors.affiliation: Department of Hepatobiliary Surgery, The Second Affiliated Hospital of Chongqing Medical University, Chongqing, People's Republic of China.
authors.forename: Chun-Mu
authors.initials: CM
authors.lastname: Miao
authors.affiliation: Department of Hepatobiliary Surgery, The Second Affiliated Hospital of Chongqing Medical University, Chongqing, People's Republic of China.
authors.forename: Ming-Xin
authors.initials: MX
authors.lastname: Ye
authors.affiliation: Department of Hepatobiliary Surgery, The Second Affiliated Hospital of Chongqing Medical University, Chongqing, People's Republic of China.
authors.forename: Ding-Pei
authors.initials: DP
authors.lastname: Xing
authors.affiliation: Department of Hepatobiliary Surgery, The Second Affiliated Hospital of Chongqing Medical University, Chongqing, People's Republic of China.
authors.forename: Kun
authors.initials: K
authors.lastname: He
authors.affiliation: Department of Hepatobiliary Surgery, The Second Affiliated Hospital of Chongqing Medical University, Chongqing, People's Republic of China.
authors.forename: Pei-Zhi
authors.initials: PZ
authors.lastname: Li
authors.affiliation: Department of Hepatobiliary and Pancreatic Surgery, Institute of Hepatobiliary and Pancreatic Diseases, School of Medicine, First Affiliated Hospital of Zhengzhou University, Zhengzhou, 450052, People's Republic of China.
authors.forename: Rong-Tao
authors.initials: RT
authors.lastname: Zhu
authors.affiliation: Department of Hepatobiliary Surgery, The Second Affiliated Hospital of Chongqing Medical University, Chongqing, People's Republic of China. Electronic address: gongjianping11@126.com.
authors.forename: Jian-Ping
authors.initials: JP
authors.lastname: Gong
mesh_terms: D000368:Aged; D000818:Animals; D001777:Blood Coagulation; D005260:Female; D005682:Gadolinium / Q000633:toxicity; D006801:Humans; D007728:Kupffer Cells / Q000502:physiology; D008107:Liver Diseases / Q000097:blood* / Q000209:etiology* / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008875:Middle Aged; D010195:Pancreatitis / Q000097:blood* / Q000150:complications* / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D013925:Thromboplastin / Q000378:metabolism*; D015854:Up-Regulation
chemical_list: D012333:RNA, Messenger; D013925:Thromboplastin; D005682:Gadolinium; C038958:gadolinium chloride
keywords: Blood coagulation system; Kupffer cells; Liver injury; Severe acute pancreatitis; Tissue factor