title: Haematopoietic effects of Angelica sinensis root cap polysaccharides against lisinopril-induced anaemia in albino rats.
abstract: BACKGROUND
  Angelica sinensis L. (Umbelliferae) has medicinal properties.
  
  
  OBJECTIVE
  The present study evaluates the haematopoietic effects of A. sinensis polysaccharides (ASP) against lisinopril-induced anaemia.
  
  
  METHODS
  Thirty healthy adult male albino rats were randomly divided into five groups (n = 6). Group I was control group. Group II was treated with angiotensin-converting enzyme inhibitor (ACEI, 20 mg/kg/day) to induce anaemia. In group III, erythropoietin (EPO, 100 IU/kg/each) was administered in combination with ACEI. Group IV was treated with ASP (1 g/kg/day), extracted from A. sinensis root caps. In Group V, ASP (1 g/kg/day) was treated with ACEI. After 28 days, blood and tissue samples were collected for haematological and histopathological analysis, respectively.
  
  
  RESULTS
  The results showed that ACEI significantly reduced the haemoglobin (Hb, 10.0 g/dL), packed cell volume (PCV, 39.5%), red blood cells (RBCs, 6.2 million/mm3), mean corpuscular volume (MCV, 53.5 fL) and mean corpuscular haemoglobin (MCH, 16.2 pg/cell) values. In the group treated with ASP, the Hb (13.7 g/dL) and RBCs (7.8 million/mm3) increased significantly (p < 0.05). The combination of ASP and ACEI led to the significant (p < 0.05) reduction in Hb (10.7 g/dL), PCV (33.3%), RBCs (6.0 million/mm3), MCV (54.42 fL) and MCH (16.44 pg/cell) values. While histopathological examination of the liver and kidney cells showed a mild degree of toxicity in the ASP-treated group.
  
  
  CONCLUSIONS
  ASP has a potentiating effect on haematological parameters when given alone. However, when administered simultaneously with lisinopril, it showed an unfavourable effect with more complicated anaemia so it should not be used with ACEIs.
authors.affiliation: a Institute of Pharmacy, Physiology and Pharmacology , University of Agriculture , Faisalabad , Pakistan.
authors.forename: Fryal
authors.initials: F
authors.lastname: Younas
authors.affiliation: a Institute of Pharmacy, Physiology and Pharmacology , University of Agriculture , Faisalabad , Pakistan.
authors.forename: Bilal
authors.initials: B
authors.lastname: Aslam
authors.affiliation: a Institute of Pharmacy, Physiology and Pharmacology , University of Agriculture , Faisalabad , Pakistan.
authors.forename: Faqir
authors.initials: F
authors.lastname: Muhammad
authors.affiliation: b Institute of Microbiology, University of Agriculture , Faisalabad , Pakistan.
authors.forename: Mashkoor
authors.initials: M
authors.lastname: Mohsin
authors.affiliation: a Institute of Pharmacy, Physiology and Pharmacology , University of Agriculture , Faisalabad , Pakistan.
authors.forename: Ahmad
authors.initials: A
authors.lastname: Raza
authors.affiliation: a Institute of Pharmacy, Physiology and Pharmacology , University of Agriculture , Faisalabad , Pakistan.
authors.forename: Muhammad Naeem
authors.initials: MN
authors.lastname: Faisal
authors.affiliation: a Institute of Pharmacy, Physiology and Pharmacology , University of Agriculture , Faisalabad , Pakistan.
authors.forename: Shamshud-Ul-
authors.initials: SU
authors.lastname: Hassan
authors.affiliation: a Institute of Pharmacy, Physiology and Pharmacology , University of Agriculture , Faisalabad , Pakistan.
authors.forename: Wafa
authors.initials: W
authors.lastname: Majeed
mesh_terms: D000740:Anemia / Q000097:blood / Q000139:chemically induced / Q000188:drug therapy*; D029971:Angelica sinensis / Q000737:chemistry*; D000818:Animals; D015415:Biomarkers / Q000097:blood; D004195:Disease Models, Animal; D004909:Erythrocyte Indices; D004912:Erythrocytes / Q000187:drug effects* / Q000378:metabolism; D004921:Erythropoietin / Q000494:pharmacology; D006397:Hematinics / Q000302:isolation & purification / Q000494:pharmacology* / Q000633:toxicity; D006400:Hematocrit; D006410:Hematopoiesis / Q000187:drug effects*; D006454:Hemoglobins / Q000378:metabolism; D041743:Herb-Drug Interactions; D017706:Lisinopril; D008297:Male; D008517:Phytotherapy; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology* / Q000633:toxicity; D018518:Plant Root Cap / Q000737:chemistry*; D010946:Plants, Medicinal; D011134:Polysaccharides / Q000302:isolation & purification / Q000494:pharmacology* / Q000633:toxicity; D017208:Rats, Wistar; D013997:Time Factors
chemical_list: D015415:Biomarkers; D006397:Hematinics; D006454:Hemoglobins; D010936:Plant Extracts; D011134:Polysaccharides; D004921:Erythropoietin; D017706:Lisinopril
keywords: ACE inhibitors; MCH; MCV; PCV; RBC count; haemoglobin level; recombinant human erythropoietin