title: Genomic sequence and virulence evaluation of MN184A-like porcine reproductive and respiratory syndrome virus in Japan.
abstract: In this study, a porcine reproductive and respiratory syndrome virus (PRRSV) that was isolated from a 9-week-old diseased pig on a farm in Japan with a high mortality rate during 2007-2008 was characterized. This unique isolate, designated as Jpn5-37, did not have a high nucleotide identity in open reading frame 5 against any Japanese isolates. Among all available type 2 PRRSV complete genome sequences, Jpn5-37 shared the highest nucleotide identity (93.6%) with virulent strain MN184A. The genomic characteristics of Jpn5-37 were highly conserved with respect to the virulent MN184A, including a continuous eight amino acid deletion in the nonstructural protein 2 region. Moreover, virus distribution, viremia and the gross and microscopic characteristics of lesions were investigated in pigs 10 days post-inoculation to elucidate the pathogenicity of the isolate. Intranasal inoculation was found to rapidly result in viremia and dissemination of the Jpn5-37 isolate to several tissues in a similar manner to EDRD1; however, the amounts of Jpn5-37 RNA in serum were significantly greater. Similarly, the quantities of Jpn5-37 viral RNA in all organs tested tended to be higher than with EDRD1 infection. Mean rectal temperatures were significantly higher in the Jpn5-37-inoculated than in the control group at 4 and 6 days post infection (dpi) and in the EDRD1-inoculated group at 6 and 8 dpi. These results suggest that the Jpn5-37 strain replicates and is more efficiently distributed to the organs than is EDRD1 under the same conditions.
authors.affiliation: Viral Disease and Epidemiological Research Division.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Iseki
authors.affiliation: Animal Research Division, Institute of Japan Association for Techno-innovation in Agriculture, Forestry and Fisheries, 446-1 Ippaizuka, Kamiyokoba, Tsukuba, Ibaraki 305-0854.
authors.forename: Takeya
authors.initials: T
authors.lastname: Morozumi
authors.affiliation: Viral Disease and Epidemiological Research Division.
authors.forename: Michihiro
authors.initials: M
authors.lastname: Takagi
authors.affiliation: Viral Disease and Epidemiological Research Division.
authors.forename: Kenji
authors.initials: K
authors.lastname: Kawashima
authors.affiliation: Pathology and Pathophysiology Research Division, National Institute of Animal Health, National Agriculture and Food Research Organization, 3-1-5 Kannondai, Tsukuba, Ibaraki, 305-0856.
authors.forename: Tomoyuki
authors.initials: T
authors.lastname: Shibahara
authors.affiliation: Animal Bioregulation Unit, Division of Animal Sciences, Institute of Agrobiological Sciences, National Agriculture and Food Research Organization, 1-2 Owashi, Tsukuba, Ibaraki 305-8634.
authors.forename: Hirohide
authors.initials: H
authors.lastname: Uenishi
authors.affiliation: Dairy Hygiene Research Division, National Institute of Animal Health, National Agriculture and Food Research Organization, Hitsujigaoka, Toyohira-ku, Sapporo, Hokkaido, 062-0045, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Tsunemitsu
mesh_terms: D000825:Animal Structures / Q000821:virology; D000818:Animals; D016000:Cluster Analysis; D016679:Genome, Viral; D007564:Japan; D010802:Phylogeny; D019318:Porcine Reproductive and Respiratory Syndrome / Q000473:pathology* / Q000821:virology*; D019316:Porcine respiratory and reproductive syndrome virus / Q000235:genetics* / Q000302:isolation & purification / Q000472:pathogenicity*; D012367:RNA, Viral / Q000097:blood / Q000235:genetics*; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D013552:Swine; D013997:Time Factors; D014759:Viral Envelope Proteins / Q000235:genetics; D014766:Viremia; D014774:Virulence
chemical_list: D012367:RNA, Viral; D014759:Viral Envelope Proteins; C114789:glycoprotein 5, PRRSV
keywords: consensus oligonucleotide reverse transcription-multiple displacement amplification; genome sequence; porcine reproductive and respiratory syndrome virus