title: Luteolibacter flavescens sp. nov., isolated from deep seawater.
abstract: A novel Gram-stain-negative, rod-shaped, non-motile strain, designated GKXT, was isolated from deep seawater. Strain GKXT was able to grow at 20-35 °C (optimum, 25 °C), pH 5.5-9.5 (optimum, 7.5) and 0-4.0 % (w/v) NaCl (optimum, 1.0 %). The major fatty acids were C16 : 1ω9c (15.4 %), C16 : 0 (18.4 %), C14 : 0 (12.0 %), iso-C14 : 0 (30.1 %) and anteiso-C15 : 0 (5.7 %). Strain GKXT contained phosphatidylethanolamine, phosphatidylmethylethanolamine, phosphatidylglycerol, diphosphatidylglycerol and an unidentified glycolipid as the main polar lipids. The only isoprenoid quinone was menaquinone-9. The diagnostic amino acids of the cell-wall peptidoglycan contained meso-diaminopimelic acid. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain GKXT belonged to the genus Luteolibacter in the family Verrucomicrobiaceae. The 16S rRNA gene sequence of this strain showed 98.0, 93.5 and 93.3 % sequence similarity, respectively, with those of Luteolibacter arcticus MC 3726T, L.uteolibacter pohnpeiensisA4T-83T and L.uteolibacter cuticulihirudinis E100T. DNA-DNA hybridization value of strain GKXT with L. arcticus MC 3726T was 33.1 %. The G+C content of the genomic DNA was 59.5 mol%. On the basis of the genotypic, phenotypic, phylogenetic and chemotaxonomic characteristics, strain GKXT was proposed to represent a novel species of the genus Luteolibacter, named Luteolibacter flavescens sp. nov. (type strain GKXT=MCCC 1K03193T=KCTC 52361T).
authors.affiliation: Ocean College, Zhejiang University, Zhoushan 316000, PR China.
authors.forename: Chongya
authors.initials: C
authors.lastname: Zhang
authors.affiliation: Department of Plant Pathology, North Carolina State University, Raleigh, NC 27695, USA.
authors.forename: Bolin
authors.initials: B
authors.lastname: Dong
authors.affiliation: Ocean College, Zhejiang University, Zhoushan 316000, PR China.
authors.forename: Ruijun
authors.initials: R
authors.lastname: Wang
authors.affiliation: Ocean College, Zhejiang University, Zhoushan 316000, PR China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Su
authors.affiliation: College of Life Sciences, Zhejiang University, Hangzhou 310058, PR China.
authors.forename: Shuaibo
authors.initials: S
authors.lastname: Han
authors.affiliation: College of Life Sciences, Zhejiang University, Hangzhou 310058, PR China.
authors.forename: Xiaoyun
authors.initials: X
authors.lastname: Yu
authors.affiliation: College of Life Sciences, Zhejiang University, Hangzhou 310058, PR China.
authors.forename: Zhe
authors.initials: Z
authors.lastname: Zhao
authors.affiliation: Ocean College, Zhejiang University, Zhoushan 316000, PR China.
authors.forename: Geyi
authors.initials: G
authors.lastname: Fu
authors.affiliation: College of Life Sciences, Zhejiang Sci-Tech University, Hangzhou 310018, PR China.
authors.forename: Cong
authors.initials: C
authors.lastname: Sun
authors.affiliation: College of Life Sciences, Zhejiang University, Hangzhou 310058, PR China.|Ocean College, Zhejiang University, Zhoushan 316000, PR China.
authors.forename: Min
authors.initials: M
authors.lastname: Wu
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D002681:China; D004269:DNA, Bacterial / Q000235:genetics; D003960:Diaminopimelic Acid / Q000737:chemistry; D005227:Fatty Acids / Q000737:chemistry; D009693:Nucleic Acid Hybridization; D010457:Peptidoglycan / Q000737:chemistry; D010743:Phospholipids / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012623:Seawater / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D061327:Verrucomicrobia / Q000145:classification* / Q000254:growth & development / Q000302:isolation & purification; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010457:Peptidoglycan; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; C073355:menaquinone 9; D003960:Diaminopimelic Acid