title: Protein Concentrate Production from Thin Stillage.
abstract: Two-stage fermentation (TSF) of saccharified wheat with a consortium of endemic lactobacilli produced CO2and induced colloid separation of fermented solution to produce a protein concentrate (PC). Protein-rich slurry (50%, db) was obtained by decanting solution or skimming floating material during or after TSF. Washing and drying processes were explored to improve protein content, extend storage life of slurry, and yield converted stillage for compound recovery. Centrifuging and washing slurry afforded a PC and clarified solution. PC protein content increased to 60% (w/w, db). The PC was dried in a spray dryer or drum dryer or tray dryer. Dried PC water activity ranged 0.23-0.30. The dried PC lysine content was low, but lysine availability (95%) was excellent. Liquid from TSF and washing was readily microfiltered. Mass recovery of protein, glycerol, 1,3-propanediol, lactic acid, acetic acid, and glycerylphosphorylcholine from combined TSF, washing, and filtration were 66, 76, 72, 77, 74, and 84%, respectively.
authors.affiliation: Department of Plant Sciences, University of Saskatchewan , 51 Campus Drive, Saskatoon, Saskatchewan S7N 5A8, Canada.
authors.forename: Kornsulee
authors.initials: K
authors.lastname: Ratanapariyanuch
authors.affiliation: Department of Plant Sciences, University of Saskatchewan , 51 Campus Drive, Saskatoon, Saskatchewan S7N 5A8, Canada.|Prairie Tide Chemicals Inc. , 102 Melville Street, Saskatoon, Saskatchewan S7J 0R1, Canada.|Guangdong Saskatchewan Oilseed Joint Laboratory, Department of Food Science and Engineering, Jinan University , 601 Huangpu Avenue West, Guangzhou, Guangdong 510632, China.
authors.forename: Youn Young
authors.identifier: http://orcid.org/0000-0002-5039-8219
authors.initials: YY
authors.lastname: Shim
authors.affiliation: Department of Plant Sciences, University of Saskatchewan , 51 Campus Drive, Saskatoon, Saskatchewan S7N 5A8, Canada.
authors.forename: Shahram
authors.initials: S
authors.lastname: Emami
authors.affiliation: Department of Plant Sciences, University of Saskatchewan , 51 Campus Drive, Saskatoon, Saskatchewan S7N 5A8, Canada.|Prairie Tide Chemicals Inc. , 102 Melville Street, Saskatoon, Saskatchewan S7J 0R1, Canada.|Guangdong Saskatchewan Oilseed Joint Laboratory, Department of Food Science and Engineering, Jinan University , 601 Huangpu Avenue West, Guangzhou, Guangdong 510632, China.
authors.forename: Martin J T
authors.initials: MJ
authors.lastname: Reaney
mesh_terms: D019342:Acetic Acid / Q000737:chemistry; D002245:Carbon Dioxide / Q000737:chemistry; D000431:Ethanol / Q000737:chemistry; D005285:Fermentation; D005374:Filtration; D005990:Glycerol / Q000737:chemistry; D005997:Glycerylphosphorylcholine / Q000737:chemistry; D019344:Lactic Acid / Q000737:chemistry; D007778:Lactobacillus / Q000378:metabolism*; D059013:Microbial Consortia; D019906:Nuclear Magnetic Resonance, Biomolecular; D010940:Plant Proteins / Q000737:chemistry*; D011409:Propylene Glycols / Q000737:chemistry; D014908:Triticum / Q000382:microbiology*
chemical_list: D010940:Plant Proteins; D011409:Propylene Glycols; D002245:Carbon Dioxide; D019344:Lactic Acid; D000431:Ethanol; C041787:1,3-propanediol; D005997:Glycerylphosphorylcholine; D005990:Glycerol; D019342:Acetic Acid
keywords: clarification; compound recovery; protein concentrate; thin stillage; two-stage fermentation