title: From Embryo to Adult: piRNA-Mediated Silencing throughout Germline Development inDrosophila.
abstract: In metazoan germ cells, transposable element activity is repressed by small noncoding PIWI-associated RNAs (piRNAs). Numerous studies inDrosophilahave elucidated the mechanism of this repression in the adult germline. However, when and how transposable element repression is established during germline development has not been addressed. Here, we show that homology-dependenttranssilencing is active in female primordial germ cells from late embryogenesis through pupal stages, and that genes related to the adult piRNA pathway are required for silencing during development. In larval gonads, we detectrhino-dependent piRNAs indicatingde novobiogenesis of functional piRNAs during development. Those piRNAs exhibit the molecular signature of the "ping-pong" amplification step. Moreover, we show that Heterochromatin Protein 1a is required for the production of piRNAs coming from telomeric transposable elements. Furthermore, as in adult ovaries, incomplete, bimodal, and stochastic repression resembling variegation can occur at all developmental stages. Clonal analysis indicates that the repression status established in embryonic germ cells is maintained until the adult stage, suggesting the implication of a cellular memory mechanism. Taken together, data presented here show that piRNAs and their associated proteins are epigenetic components of a continuous repression system throughout germ cell development.
authors.affiliation: Laboratoire de Biologie du Développement, Sorbonne Universités, Université Pierre et Marie Curie, Centre National de la Recherche Scientifique, Institut de Biologie Paris-Seine, F-75005, France.
authors.forename: Pauline P
authors.initials: PP
authors.lastname: Marie
authors.affiliation: Laboratoire de Biologie du Développement, Sorbonne Universités, Université Pierre et Marie Curie, Centre National de la Recherche Scientifique, Institut de Biologie Paris-Seine, F-75005, France stephane.ronsseray@upmc.fr antoine.boivin@upmc.fr.
authors.forename: Stéphane
authors.initials: S
authors.lastname: Ronsseray
authors.affiliation: Laboratoire de Biologie du Développement, Sorbonne Universités, Université Pierre et Marie Curie, Centre National de la Recherche Scientifique, Institut de Biologie Paris-Seine, F-75005, France stephane.ronsseray@upmc.fr antoine.boivin@upmc.fr.
authors.forename: Antoine
authors.identifier: 0000-0001-8671-1599
authors.initials: A
authors.lastname: Boivin
mesh_terms: D000818:Animals; D002868:Chromosomal Proteins, Non-Histone / Q000235:genetics*; D004251:DNA Transposable Elements / Q000235:genetics; D029721:Drosophila Proteins / Q000235:genetics*; D004331:Drosophila melanogaster / Q000235:genetics* / Q000254:growth & development; D047108:Embryonic Development; D044127:Epigenesis, Genetic / Q000235:genetics; D018507:Gene Expression Regulation, Developmental; D020868:Gene Silencing; D005854:Germ Cells / Q000254:growth & development* / Q000378:metabolism; D006066:Gonads / Q000254:growth & development / Q000378:metabolism; D007814:Larva / Q000235:genetics* / Q000254:growth & development; D034741:RNA, Small Interfering / Q000235:genetics*
chemical_list: D002868:Chromosomal Proteins, Non-Histone; D004251:DNA Transposable Elements; D029721:Drosophila Proteins; D034741:RNA, Small Interfering; C452193:heterochromatin protein 1, Drosophila
keywords: Drosophila; development; germline; piRNA; silencing