title: Ameliorative effects of rutin against metabolic, biochemical and hormonal disturbances in polycystic ovary syndrome in rats.
abstract: BACKGROUND
  Polycystic ovary syndrome (PCOS) is the most prevalent endocrinopathy in women of reproductive age. The study was commenced to assess the favorable effects of Rutin against metabolic, biochemical, histological, and androgenic aspects of polycystic ovary syndrome in rats.
  
  
  METHODS
  Female Sprague-Dawley rats were administered letrozole (1 mg/kg) per orally (p.o) for a period of 21 days for the induction of PCOS, followed by dose of rutin (100 mg/kg and 150 mg/kg, p.o) for 15 days using 0.5% w/v CMC as vehicle. Metformin was also given as a standard control to one of the rat groups. Serum estradiol, progesterone, testosterone, serum lipid parameters, CRP and glucose levels were evaluated. Furthermore, antioxidant activity was tested using superoxide dismutase, catalase, glutathione per-oxidase and reactive-oxygen species level.
  
  
  RESULTS
  Rutin flavonoid had a dose-dependent effect on androgenic levels depicting more recovery in the rutin-I treated group, while rutin-II treated groups showed better antioxidant and lipid profiles as compared with PCOS groups. A decrease in the value of C reactive protein (CRP) and a restoration in the proportion of estrous phase smears were observed in the rutin treated groups. Histopathological examination of ovary revealed a significant decrease in the number of cystic follicles in post treated groups. The effects observed with rutin were moderately similar to that with standard metformin, a widely used treatment drug for PCOS.
  
  
  CONCLUSIONS
  The study provides evidence for the potential ameliorative effects of rutin against clinical and biochemical features of PCOS.
authors.affiliation: Reproductive Physiology Laboratory, Department Of Animal Sciences, Quaid-i-Azam University, Islamabad, Pakistan.
authors.forename: Sarwat
authors.initials: S
authors.lastname: Jahan
authors.affiliation: Reproductive Physiology Laboratory, Department Of Animal Sciences, Quaid-i-Azam University, Islamabad, Pakistan.
authors.forename: Faryal
authors.initials: F
authors.lastname: Munir
authors.affiliation: Reproductive Physiology Laboratory, Department Of Animal Sciences, Quaid-i-Azam University, Islamabad, Pakistan. ruhail12345@yahoo.com.|Department of Community Health Sciences, College of Applied Medical Sciences, King Saud University, Riyadh, Saudi Arabia. ruhail12345@yahoo.com.
authors.forename: Suhail
authors.initials: S
authors.lastname: Razak
authors.affiliation: Reproductive Physiology Laboratory, Department Of Animal Sciences, Quaid-i-Azam University, Islamabad, Pakistan.
authors.forename: Anam
authors.initials: A
authors.lastname: Mehboob
authors.affiliation: Reproductive Physiology Laboratory, Department Of Animal Sciences, Quaid-i-Azam University, Islamabad, Pakistan.
authors.forename: Qurat Ul
authors.initials: QU
authors.lastname: Ain
authors.affiliation: Reproductive Physiology Laboratory, Department Of Animal Sciences, Quaid-i-Azam University, Islamabad, Pakistan.
authors.forename: Hizb
authors.initials: H
authors.lastname: Ullah
authors.affiliation: Department of Biochemistry, Quaid-i-Azam University, Islamabad, Pakistan.
authors.forename: Tayyaba
authors.initials: T
authors.lastname: Afsar
authors.affiliation: Reproductive Physiology Laboratory, Department Of Animal Sciences, Quaid-i-Azam University, Islamabad, Pakistan.
authors.forename: Ghazala
authors.initials: G
authors.lastname: Shaheen
authors.affiliation: Department of Community Health Sciences, College of Applied Medical Sciences, King Saud University, Riyadh, Saudi Arabia.
authors.forename: Ali
authors.initials: A
authors.lastname: Almajwal
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D047072:Aromatase Inhibitors / Q000008:administration & dosage; D015415:Biomarkers; D001786:Blood Glucose / Q000187:drug effects; D001837:Body Weights and Measures; D002097:C-Reactive Protein / Q000378:metabolism; D004195:Disease Models, Animal; D030762:Estrous Cycle / Q000187:drug effects; D005260:Female; D042341:Gonadal Hormones / Q000097:blood / Q000378:metabolism; D000077289:Letrozole; D008055:Lipids / Q000097:blood; D008687:Metformin / Q000494:pharmacology; D009570:Nitriles / Q000008:administration & dosage; D010053:Ovary / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology; D018384:Oxidative Stress / Q000187:drug effects; D011085:Polycystic Ovary Syndrome / Q000188:drug therapy / Q000378:metabolism* / Q000473:pathology; D051381:Rats; D017382:Reactive Oxygen Species / Q000378:metabolism; D012431:Rutin / Q000494:pharmacology*; D014230:Triazoles / Q000008:administration & dosage
chemical_list: D000975:Antioxidants; D047072:Aromatase Inhibitors; D015415:Biomarkers; D001786:Blood Glucose; D042341:Gonadal Hormones; D008055:Lipids; D009570:Nitriles; D017382:Reactive Oxygen Species; D014230:Triazoles; D012431:Rutin; D000077289:Letrozole; D002097:C-Reactive Protein; D008687:Metformin
keywords: Letrozole; Oxidative stress; Polycystic ovary syndrome; Rutin