title: Room temperature ionic liquids-based salting-in strategy for counter-current chromatography in the separation of arctiin.
abstract: Counter-current chromatography (CCC) is a solid support-free liquid-liquid partition chromatography and has wide applications. However, CCC separation is still a challenging process and the selection of appropriate solvent system for separation of target compound(s) is still relatively time-consuming. In this work, we introduced a room temperature ionic liquids-based salting-in strategy for the rapid selection of suitable solvent systems for CCC separation. In the randomly selected solvent systems, such as ethyl acetate-water, n-butanol-water, n-pentanol-water, n-hexanol-water, and n-octanol-water, several ionic liquids such as [AMIM]Cl, [MAMIM]Cl, and [BMIM]Cl can increase the solubility of the solutes in the lower phase, which made a dose-dependent decreasing of partition coefficient of solute in the two-phase solvent system. Thus, it is possible to get a suitable solvent system with sweet K spot such as K=1 only by adding some ionic liquids into the systems. As an example, arctiin, a bioactive lignin component of the fruit of Arctium lappa. L. (Niubangzi in Chinese), was selected and successfully separated by CCC with room temperature ionic liquids-based n-butanol-water systems. It seems a very efficient alternative strategy for the optimization of solvent systems for CCC separation of natural products.
authors.affiliation: Research Center of Siyuan Natural Pharmacy and Biotoxicology, College of Life Sciences, Zhejiang University, Hangzhou, Zhejiang Province, 310058, China.
authors.forename: Yanyan
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Research Center of Siyuan Natural Pharmacy and Biotoxicology, College of Life Sciences, Zhejiang University, Hangzhou, Zhejiang Province, 310058, China.
authors.forename: Lihong
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Research Center of Siyuan Natural Pharmacy and Biotoxicology, College of Life Sciences, Zhejiang University, Hangzhou, Zhejiang Province, 310058, China.
authors.forename: Dingding
authors.initials: D
authors.lastname: Wang
authors.affiliation: Research Center of Siyuan Natural Pharmacy and Biotoxicology, College of Life Sciences, Zhejiang University, Hangzhou, Zhejiang Province, 310058, China.
authors.forename: Xiuyun
authors.initials: X
authors.lastname: Guo
authors.affiliation: Research Center of Siyuan Natural Pharmacy and Biotoxicology, College of Life Sciences, Zhejiang University, Hangzhou, Zhejiang Province, 310058, China. Electronic address: drwushihua@zju.edu.cn.
authors.forename: Shihua
authors.initials: S
authors.lastname: Wu
mesh_terms: D031170:Arctium / Q000737:chemistry; D003377:Countercurrent Distribution / Q000379:methods*; D005663:Furans / Q000302:isolation & purification*; D005960:Glucosides / Q000302:isolation & purification*; D052578:Ionic Liquids / Q000737:chemistry*; D012997:Solvents / Q000737:chemistry; D013696:Temperature
chemical_list: D005663:Furans; D005960:Glucosides; D052578:Ionic Liquids; D012997:Solvents; C077992:arctiin
keywords: Arctiin; Natural product isolation; Room temperature ionic liquids; Salting-in; Salting-out; Solvent selection