title: Noninvasive Imaging of Colitis Using Multispectral Optoacoustic Tomography.
abstract: Currently, several noninvasive modalities, including MRI and PET, are being investigated to identify early intestinal inflammation, longitudinally monitor disease status, or detect dysplastic changes in patients with inflammatory bowel disease. Here, we assess the applicability and utility of multispectral optoacoustic tomography (MSOT) in evaluating the presence and severity of colitis.Methods:C57B/6 mice were untreated or treated withBacteroides fragilisand antibiotic-mediated depletion of intestinal flora to initiate colitis. Mice were imaged using MSOT to detect intestinal inflammation. Intestinal inflammation identified with MSOT was also confirmed using both colonoscopy and histology.Results:Mice with bacterial colitis demonstrated a temporally associated increase in mesenteric and colonic vascularity with an increase in mean signal intensity of oxygenated hemoglobin (P= 0.004) by MSOT 2 d after inoculation. These findings were significantly more prominent 7 d after inoculation, with increased mean signal intensity of oxygenated hemoglobin (P= 0.0002) and the development of punctate vascular lesions on the colonic surface, which corresponded to changes observed on colonoscopy as well as histology.Conclusion:With improvements in depth of tissue penetration, MSOT may hold potential as a sensitive, accurate, noninvasive imaging tool in the evaluation of patients with inflammatory bowel disease.
authors.affiliation: Department of Surgery, University of Louisville, Louisville, Kentucky.
authors.forename: Neal
authors.initials: N
authors.lastname: Bhutiani
authors.affiliation: Department of Pathology, University of Alabama Birmingham, Birmingham, Alabama.
authors.forename: William E
authors.initials: WE
authors.lastname: Grizzle
authors.affiliation: Department of Surgery, University of Louisville, Louisville, Kentucky.
authors.forename: Susan
authors.initials: S
authors.lastname: Galandiuk
authors.affiliation: Department of Pathology, University of Alabama Birmingham, Birmingham, Alabama.
authors.forename: Denis
authors.initials: D
authors.lastname: Otali
authors.affiliation: Department of Surgery, University of Louisville, Louisville, Kentucky.
authors.forename: Gerald W
authors.initials: GW
authors.lastname: Dryden
authors.affiliation: Department of Microbiology and Immunology, University of Louisville, Louisville, Kentucky; and.
authors.forename: Nejat K
authors.initials: NK
authors.lastname: Egilmez
authors.affiliation: Department of Cancer Biology, Wake Forest Baptist Comprehensive Cancer Center, Winston-Salem, North Carolina lacey_mcnally@hotmail.com.
authors.forename: Lacey R
authors.initials: LR
authors.lastname: McNally
mesh_terms: D000818:Animals; D001424:Bacterial Infections / Q000000981:diagnostic imaging*; D003092:Colitis / Q000000981:diagnostic imaging*; D051379:Mice; D008810:Mice, Inbred C57BL; D061088:Photoacoustic Techniques; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D014054:Tomography / Q000379:methods*
keywords: animal imaging; colitis; gastrointestinal; inflammatory bowel disease; molecular imaging; multispectral optoacoustic tomography; oxygenated hemoglobin