title: Analysis of Ras/ERK Compartmentalization by Subcellular Fractionation.
abstract: A vast number of stimuli use the Ras/Raf/MEK/ERK signaling cascade to transmit signals from their cognate receptors, in order to regulate multiple cellular functions, including key processes such as proliferation, cell cycle progression, differentiation, and survival. The duration, intensity and specificity of the responses are, in part, controlled by the compartmentalization/subcellular localization of the signaling intermediaries. Ras proteins are found in different plasma membrane microdomains and endomembranes. At these localizations, Ras is subject to site-specific regulatory mechanisms, distinctively engaging effector pathways and switching-on diverse genetic programs to generate a multitude of biological responses. The Ras effector pathway leading to ERKs activation is also subject to space-related regulatory processes. About half of ERK1/2 substrates are found in the nucleus and function mainly as transcription factors. The other half resides in the cytosol and other cellular organelles. Such subcellular distribution enhances the complexity of the Ras/ERK cascade and constitutes an essential mechanism to endow variability to its signals, which enables their participation in the regulation of a broad variety of functions. Thus, analyzing the subcellular compartmentalization of the members of the Ras/ERK cascade constitutes an important factor to be taken into account when studying specific biological responses evoked by Ras/ERK signals. Herein, we describe methods for such purpose.
authors.affiliation: Instituto de Biomedicina y Biotecnología de Cantabria, Consejo Superior de Investigaciones Científicas - Universidad de Cantabria, c/Albert Einstein, 22, PCTCAN, Santander, 39011, Cantabria, Spain.
authors.forename: Lorena
authors.initials: L
authors.lastname: Agudo-Ibañez
authors.affiliation: Instituto de Biomedicina y Biotecnología de Cantabria, Consejo Superior de Investigaciones Científicas - Universidad de Cantabria, c/Albert Einstein, 22, PCTCAN, Santander, 39011, Cantabria, Spain.
authors.forename: Piero
authors.initials: P
authors.lastname: Crespo
authors.affiliation: Instituto de Biomedicina y Biotecnología de Cantabria, Consejo Superior de Investigaciones Científicas - Universidad de Cantabria, c/Albert Einstein, 22, PCTCAN, Santander, 39011, Cantabria, Spain. casarb@unican.es.
authors.forename: Berta
authors.initials: B
authors.lastname: Casar
mesh_terms: D000818:Animals; D002458:Cell Fractionation / Q000379:methods; D002460:Cell Line; D002467:Cell Nucleus / Q000378:metabolism; D004721:Endoplasmic Reticulum / Q000378:metabolism; D048049:Extracellular Signal-Regulated MAP Kinases / Q000302:isolation & purification / Q000378:metabolism*; D042541:Intracellular Space / Q000378:metabolism; D021962:Membrane Microdomains / Q000378:metabolism; D021381:Protein Transport; D013347:Subcellular Fractions; D018631:ras Proteins / Q000302:isolation & purification / Q000378:metabolism*
chemical_list: D048049:Extracellular Signal-Regulated MAP Kinases; D018631:ras Proteins
keywords: ERK; MAP kinases; Membrane microdomains; Ras; Spatial regulation of signaling; Subcellular fractionation