title: Molecular surveillance of dengue in Minas Gerais provides insights on dengue virus 1 and 4 circulation in Brazil.
abstract: Dengue, caused by any of the four types of Dengue virus (DENV) is the most important arbovirus in the world. In this study we performed a molecular surveillance of dengue during the greatest dengue outbreak that took place in Divinópolis, Minas Gerais state, Southeast Brazil, in 2013. Samples from 100 patients with clinical symptoms of dengue were studied and 26 were positive. The capsid/premembrane (CprM) and envelope gene sequences of some samples were amplified and sequenced. Molecular analyses demonstrated that two DENV-1 lineages, belonging to genotype V were introduced and co-circulated in Divinópolis. When compared to each other, those lineages presented high genetic diversity and showed unique amino acids substitutions in the envelope protein, including in domains I, II, and III. DENV-4 strains from Divinópolis clustered within genotype IIb and the most recent common ancestor was probably introduced into the city three years before the 2013 epidemic. Here we demonstrated for the first time the circulation of DENV-4 and the co-circulation of two DENV-1 lineages in Midwest region of Minas Gerais, Brazil. Moreover our analysis indicated the introduction of five DENV-1 lineages, genotype V into Brazil, in different times. J. Med. Virol. 89:966-973, 2017. © 2016 Wiley Periodicals, Inc.
authors.affiliation: Campus Centro-Oeste Dona Lindu, Universidade Federal de São João Del Rei (UFSJ), Divinópolis, Minas Gerais, Brazil.
authors.forename: Karina Rocha
authors.initials: KR
authors.lastname: Dutra
authors.affiliation: Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, Minas Gerais, Brazil.|Instituto de Ciências Biológicas, Universidade Federal de Juiz de Fora (UFJF), Juiz de Fora, Minas Gerais, Brazil.
authors.forename: Betânia Paiva
authors.initials: BP
authors.lastname: Drumond
authors.affiliation: Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, Minas Gerais, Brazil.|Instituto de Ciências Biológicas, Universidade Federal de Juiz de Fora (UFJF), Juiz de Fora, Minas Gerais, Brazil.
authors.forename: Izabela Maurício
authors.initials: IM
authors.lastname: de Rezende
authors.affiliation: Faculdade de Medicina de São Jose do Rio Preto (FAMERP), São Jose do Rio Preto, São Paulo, Brazil.
authors.forename: Maurício Lacerda
authors.initials: ML
authors.lastname: Nogueira
authors.affiliation: Campus Centro-Oeste Dona Lindu, Universidade Federal de São João Del Rei (UFSJ), Divinópolis, Minas Gerais, Brazil.
authors.forename: Débora
authors.initials: D
authors.lastname: de Oliveira Lopes
authors.affiliation: Centro de Pesquisas René Rachou (FIOCRUZ), Belo Horizonte, Minas Gerais, Brazil.
authors.forename: Carlos Eduardo
authors.initials: CE
authors.lastname: Calzavara Silva
authors.affiliation: Campus Centro-Oeste Dona Lindu, Universidade Federal de São João Del Rei (UFSJ), Divinópolis, Minas Gerais, Brazil.
authors.forename: Jaqueline Maria
authors.initials: JM
authors.lastname: Siqueira Ferreira
authors.affiliation: Campus Centro-Oeste Dona Lindu, Universidade Federal de São João Del Rei (UFSJ), Divinópolis, Minas Gerais, Brazil.
authors.forename: Luciana Lara
authors.initials: LL
authors.lastname: Dos Santos
mesh_terms: D019943:Amino Acid Substitution; D001938:Brazil / Q000453:epidemiology; D016000:Cluster Analysis; D003715:Dengue / Q000453:epidemiology* / Q000821:virology*; D003716:Dengue Virus / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D062665:Epidemiological Monitoring; D014644:Genetic Variation; D005838:Genotype; D006801:Humans; D017720:Molecular Epidemiology; D009154:Mutation; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D015678:Viral Structural Proteins / Q000235:genetics
chemical_list: D015678:Viral Structural Proteins
keywords: dengue virus; dynamics; evolution; genotype; lineage; phylogenetic analysis