title: DNA replication after mutagenic treatment in Hordeum vulgare.
abstract: The temporal and spatial properties of DNA replication in plants related to DNA damage and mutagenesis is poorly understood. Experiments were carried out to explore the relationships between DNA replication, chromatin structure and DNA damage in nuclei from barley root tips. We quantitavely analysed the topological organisation of replication foci using pulse EdU labelling during the S phase and its relationship with the DNA damage induced by mutagenic treatment with maleic hydrazide (MH), nitroso-N-methyl-urea (MNU) and gamma ray. Treatment with mutagens did not change the characteristic S-phase patterns in the nuclei; however, the frequencies of the S-phase-labelled cells after treatment differed from those observed in the control cells. The analyses of DNA replication in barley nuclei were extended to the micronuclei induced by mutagens. Replication in the chromatin of the micronuclei was rare. The results of simultanous TUNEL reaction to identify cells with DNA strand breaks and the labelling of the S-phase cells with EdU revealed the possibility of DNA replication occurring in damaged nuclei. For the first time, the intensity of EdU fluorescence to study the rate of DNA replication was analysed.
authors.affiliation: Department of Plant Anatomy and Cytology, University of Silesia, Jagiellonska 28, 40-032 Katowice, Poland. Electronic address: jolanta.kwasniewska@us.edu.pl.
authors.forename: Jolanta
authors.initials: J
authors.lastname: Kwasniewska
authors.affiliation: Department of Plant Anatomy and Cytology, University of Silesia, Jagiellonska 28, 40-032 Katowice, Poland.
authors.forename: Arita
authors.initials: A
authors.lastname: Kus
authors.affiliation: Department of Plant Anatomy and Cytology, University of Silesia, Jagiellonska 28, 40-032 Katowice, Poland.
authors.forename: Monika
authors.initials: M
authors.lastname: Swoboda
authors.affiliation: Department of Plant Anatomy and Cytology, University of Silesia, Jagiellonska 28, 40-032 Katowice, Poland.
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Braszewska-Zalewska
mesh_terms: D002467:Cell Nucleus / Q000187:drug effects; D004249:DNA Damage / Q000187:drug effects*; D004261:DNA Replication / Q000187:drug effects*; D018744:DNA, Plant / Q000187:drug effects*; D005720:Gamma Rays; D001467:Hordeum / Q000187:drug effects* / Q000235:genetics*; D020287:In Situ Nick-End Labeling / Q000379:methods; D008300:Maleic Hydrazide / Q000633:toxicity; D016296:Mutagenesis / Q000187:drug effects*; D009153:Mutagens / Q000633:toxicity*
chemical_list: D018744:DNA, Plant; D009153:Mutagens; D008300:Maleic Hydrazide
keywords: Barley; DNA replication; EdU; Micronuclei; Mutagens