title: Renaissance of protein crystallization and precipitation in biopharmaceuticals purification.
abstract: The current chromatographic approaches used in protein purification are not keeping pace with the increasing biopharmaceutical market demand. With the upstream improvements, the bottleneck shifted towards the downstream process. New approaches rely in Anything But Chromatography methodologies and revisiting former techniques with a bioprocess perspective. Protein crystallization and precipitation methods are already implemented in the downstream process of diverse therapeutic biological macromolecules, overcoming the current chromatographic bottlenecks. Promising work is being developed in order to implement crystallization and precipitation in the purification pipeline of high value therapeutic molecules. This review focuses in the role of these two methodologies in current industrial purification processes, and highlights their potential implementation in the purification pipeline of high value therapeutic molecules, overcoming chromatographic holdups.
authors.affiliation: UCIBIO, REQUIMTE, Departamento de Química, Faculdade de Ciências e Tecnologia, Universidade NOVA de Lisboa, 2829-516 Caparica, Portugal.
authors.forename: Raquel
authors.initials: R
authors.lastname: Dos Santos
authors.affiliation: UCIBIO, REQUIMTE, Departamento de Química, Faculdade de Ciências e Tecnologia, Universidade NOVA de Lisboa, 2829-516 Caparica, Portugal. Electronic address: almc@fct.unl.pt.
authors.forename: Ana Luísa
authors.initials: AL
authors.lastname: Carvalho
authors.affiliation: UCIBIO, REQUIMTE, Departamento de Química, Faculdade de Ciências e Tecnologia, Universidade NOVA de Lisboa, 2829-516 Caparica, Portugal. Electronic address: cecilia.roque@fct.unl.pt.
authors.forename: A Cecília A
authors.initials: AC
authors.lastname: Roque
mesh_terms: D001702:Biopharmaceutics; D011232:Chemical Precipitation; D003460:Crystallization; D011506:Proteins / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification
chemical_list: D011506:Proteins
keywords: Anything but chromatography; Biopharmaceutical; Crystallization; Downstream process; Precipitation; Protein purification