title: A comparative study for lipase immobilization onto alginate based composite electrospun nanofibers with effective and enhanced stability.
abstract: In this study, lipase was successfully immobilized on polyvinyl alcohol/alginate and polyethylene oxide/alginate nanofibers that were prepared by electrospinning. Results showed that nanofibers (especially polyvinyl alcohol/alginate) enhanced the stability properties of lipase. When the free lipase lost its all activity after 40-60min at high temperatures, both lipase immobilized nanofibers kept almost 65-70% activity at the same time. The lipase immobilized poly vinyl alcohol/alginate and polyethylene oxide/alginate nanofibers protected approximately all of their activities until pH 9. Lipase immobilized polyvinyl alcohol/alginate and polyethylene oxide/alginate nanofibers maintained 60% of their activities after 14 and 7 reuses, respectively. The morphology of nanofibers was characterized by Scanning Electron Microscope, Fourier Transform Infrared Spectroscopy and Thermal Gravimetric Analyzer. As a result, this nanofiber production method, electrospinning, is simple, versatile and economical for preparing appropriate carrier to immobilize the enzymes.
authors.affiliation: Muğla Sıtkı Koçman University, Faculty of Science, Chemistry Department, Muğla, Turkey. Electronic address: y-ispirli@hotmail.com.
authors.forename: Yasemin
authors.initials: Y
authors.lastname: İspirli Doğaç
authors.affiliation: Selçuk University, Vocational School Of Technical Sciences, Chemistry and Chemical Processing Technologies, Konya, Turkey. Electronic address: deveci-ilyas@hotmail.com.
authors.forename: İlyas
authors.initials: İ
authors.lastname: Deveci
authors.affiliation: Necmettin Erbakan University, Ahmet Kelesoglu Faculty of Educational Sciences, Department of Mathematics and Science Education, Konya, Turkey. Electronic address: bmercimek@konya.edu.tr.
authors.forename: Bedrettin
authors.initials: B
authors.lastname: Mercimek
authors.affiliation: Muğla Sıtkı Koçman University, Faculty of Science, Chemistry Department, Muğla, Turkey. Electronic address: mteke@mu.edu.tr.
authors.forename: Mustafa
authors.initials: M
authors.lastname: Teke
mesh_terms: D000464:Alginates / Q000737:chemistry*; D000818:Animals; D004560:Electricity; D004795:Enzyme Stability; D004800:Enzymes, Immobilized / Q000737:chemistry*; D020723:Glucuronic Acid / Q000737:chemistry; D006603:Hexuronic Acids / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008049:Lipase / Q000737:chemistry*; D057139:Nanofibers / Q000737:chemistry*; D036103:Nanotechnology; D011092:Polyethylene Glycols / Q000737:chemistry; D011142:Polyvinyl Alcohol / Q000737:chemistry*; D013552:Swine; D013696:Temperature
chemical_list: D000464:Alginates; D004800:Enzymes, Immobilized; D006603:Hexuronic Acids; D011092:Polyethylene Glycols; D020723:Glucuronic Acid; D011142:Polyvinyl Alcohol; D008049:Lipase
keywords: Alginate; Composite; Electrospinning; Immobilization; Lipase; Nanofiber