title: The variation of macro- and micro-minerals of tissues in diabetic and non-diabetic rats.
abstract: This study determined the levels of Ca, Mg, Fe, Zn, Cu, and Na in various tissues samples (liver, brain, kidney, intestines, muscle and hair) of diabetic and non-diabetic rats by flame atomic absorption spectroscopy, in order to assess the role of element levels during T2DM. The ratios of Ca/Mg, Zn/Cu, Ca/Zn, and Mg/Zn in diabetic and non-diabetic rat tissues were also calculated. The determined element levels were further subjected to a student-t test statistical analysis and multiple-linear-regression in order to evaluate similarities, differences, and an inter-element association in tissues of diabetic and non-diabetic rats. The results of the study showed high variability in element levels and Ca/Mg Zn/Cu Mg/Zn Ca/Zn ratios in the tissues of diabetic and non-diabetic rats, but are tissue- and element-dependent, suggesting differences in the accumulation of the elements in tissues of diabetics and non-diabetics. The obtained significant differences in the levels of elements and Ca/Mg Zn/Cu Mg/Zn Ca/Zn ratios in several tissues of diabetic and non-diabetic rats in this study suggest that the investigated elements play considerable roles in the T2DM disease process. Strong inter-element associations (R2≥0.9) were observed for some elements in tissues of diabetic and non-diabetics rats. However, poor inter-elemental associations were obtained for some elements in the tissues of diabetic and non-diabetic rats.
authors.affiliation: Department of Chemistry, Winston-Salem State University, Winston-Salem, NC, 27110, USA; Biomedical Research Infrastructure Center, Winston Salem State University, Winston-Salem, NC 27110, USA.
authors.forename: Tennille D
authors.initials: TD
authors.lastname: Presley
authors.affiliation: Department of Physiology and Pharmacology, Wake Forest University, Winston-Salem, NC, USA.
authors.forename: A'ja V
authors.initials: AV
authors.lastname: Duncan
authors.affiliation: Biomedical Research Infrastructure Center, Winston Salem State University, Winston-Salem, NC 27110, USA.
authors.forename: Anne B
authors.initials: AB
authors.lastname: Jeffers
authors.affiliation: Department of Chemistry, North Carolina A&T State University, Greensboro, NC 27411, USA. Electronic address: sofakayo@ncat.edu.
authors.forename: Sayo O
authors.initials: SO
authors.lastname: Fakayode
mesh_terms: D000818:Animals; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism*; D003924:Diabetes Mellitus, Type 2 / Q000378:metabolism*; D016014:Linear Models; D008297:Male; D008903:Minerals / Q000032:analysis* / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D014018:Tissue Distribution
chemical_list: D008903:Minerals
keywords: Calcium/Magnesium and Zinc/Copper ratios; Macro- and micro-elements status; Rats; Tissues; Type II diabetes mellitus