title: Extending a Single Residue Switch for Abbreviating Catalysis in Plantent-Kaurene Synthases.
abstract: Production ofent-kaurene as a precursor for important signaling molecules such as the gibberellins seems to have arisen early in plant evolution, with corresponding cyclase(s) present in all land plants (i.e., embryophyta). The relevant enzymes seem to represent fusion of the class II diterpene cyclase that produces the intermediateent-copalyl diphosphate (ent-CPP) and the subsequently acting class I diterpene synthase that producesent-kaurene, although the bifunctionality of the ancestral gene is only retained in certain early diverging plants, with gene duplication and sub-functionalization leading to distinctent-CPP synthases andent-kaurene synthases (KSs) generally observed. This evolutionary scenario implies that plant KSs should have conserved structural features uniquely required for production ofent-kaurene relative to related enzymes that have alternative function. Notably, substitution of threonine for a conserved isoleucine has been shown to "short-circuit" the complex bicyclization and rearrangement reaction catalyzed by KSs after initial cyclization, leading to predominant production ofent-pimaradiene, at least in KSs from angiosperms. Here this effect is shown to extend to KSs from earlier diverging plants (i.e., bryophytes), including a bifunctional/KS. In addition, attribution of the dramatic effect of this single residue "switch" on product outcome to electrostatic stabilization of theent-pimarenyl carbocation intermediate formed upon initial cyclization by the hydroxyl introduced by threonine substitution has been called into question by the observation of similar effects from substitution of alanine. Here further mutational analysis and detailed product analysis is reported that supports the importance of electrostatic stabilization by a hydroxyl or water.
authors.affiliation: Roy J. Carver Department of Biochemistry, Biophysics and Molecular Biology, Iowa State University, Ames IA, USA.
authors.forename: Meirong
authors.initials: M
authors.lastname: Jia
authors.affiliation: Roy J. Carver Department of Biochemistry, Biophysics and Molecular Biology, Iowa State University, Ames IA, USA.
authors.forename: Reuben J
authors.initials: RJ
authors.lastname: Peters
keywords: carbocation; catalytic mechanism; diterpene synthases; diterpenoids; metabolic engineering; mutagenesis; product specificity