title: Effect of 24-epibrassinolide on Brassica napus alternative respiratory pathway, guard cells movements and phospholipid signaling under salt stress.
abstract: Using Brassica napus roots we observed statistically significant increase in alternative respiratory pathway in response to exogenous 24-epibrassinolide (EBL) under optimal conditions and salinity. Also we observed activation of phospholipid signaling under the same conditions in response to EBL by measuring levels of lipid second messengers - diacylglycerol (DAG) and phosphatidic acid (PA). We found that brassinosteroids cause closure of stomata in isolated leaf disks while inhibitors of alternative oxidase cancelled these effects. This study demonstrates that BRs activate total respiration rate, alternative respiratory pathway, production of PA and DAG, stimulate stomata closure and growth under optimal conditions and salinity. Also, specific inhibitor of brassinosteroids biosynthesis decreased alternative respiratory pathway and production of lipid messengers in rape plants.
authors.affiliation: Department of the Molecular Mechanisms of Cell Metabolism Regulation, Institute of Bioorganic Chemistry and Petrochemistry, The National Academy of Sciences of Ukraine, 02660, Murmanska str., 1, Kyiv, Ukraine.
authors.forename: Michael
authors.initials: M
authors.lastname: Derevyanchuk
authors.affiliation: Department of the Molecular Mechanisms of Cell Metabolism Regulation, Institute of Bioorganic Chemistry and Petrochemistry, The National Academy of Sciences of Ukraine, 02660, Murmanska str., 1, Kyiv, Ukraine.
authors.forename: Sergii
authors.initials: S
authors.lastname: Kretynin
authors.affiliation: Department of the Molecular Mechanisms of Cell Metabolism Regulation, Institute of Bioorganic Chemistry and Petrochemistry, The National Academy of Sciences of Ukraine, 02660, Murmanska str., 1, Kyiv, Ukraine.
authors.forename: Oksana
authors.initials: O
authors.lastname: Iakovenko
authors.affiliation: Laboratory of Steroid Chemistry, Institute of Bioorganic Chemistry, The National Academy of Sciences of Belarus, 220141, Kuprevich str., 5, Minsk, Belarus.
authors.forename: Raisa
authors.initials: R
authors.lastname: Litvinovskaya
authors.affiliation: Laboratory of Steroid Chemistry, Institute of Bioorganic Chemistry, The National Academy of Sciences of Belarus, 220141, Kuprevich str., 5, Minsk, Belarus.
authors.forename: Vladimir
authors.initials: V
authors.lastname: Zhabinskii
authors.affiliation: Department of Biology, Faculty of Sciences, Jan Evangelista Purkyne University, Usti nad Labem, Czech Republic.
authors.forename: Jan
authors.initials: J
authors.lastname: Martinec
authors.affiliation: Institute of Food Biotechnology and Genomics, National Academy of Sciences of Ukraine, Osypovskogo 2a, Kyiv 04123, Ukraine.
authors.forename: Yaroslav
authors.initials: Y
authors.lastname: Blume
authors.affiliation: Laboratory of Steroid Chemistry, Institute of Bioorganic Chemistry, The National Academy of Sciences of Belarus, 220141, Kuprevich str., 5, Minsk, Belarus.
authors.forename: Vladimir
authors.initials: V
authors.lastname: Khripach
authors.affiliation: Department of the Molecular Mechanisms of Cell Metabolism Regulation, Institute of Bioorganic Chemistry and Petrochemistry, The National Academy of Sciences of Ukraine, 02660, Murmanska str., 1, Kyiv, Ukraine. Electronic address: kravets@bpci.kiev.ua.
authors.forename: Volodymyr
authors.initials: V
authors.lastname: Kravets
mesh_terms: D029688:Brassica napus / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*; D060406:Brassinosteroids / Q000494:pharmacology*; D004075:Diglycerides / Q000378:metabolism; D024101:Mitochondrial Proteins / Q000378:metabolism; D010088:Oxidoreductases / Q000378:metabolism; D010712:Phosphatidic Acids / Q000378:metabolism; D018515:Plant Leaves / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism; D010940:Plant Proteins / Q000378:metabolism; D018517:Plant Roots / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism; D054046:Plant Stomata / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism; D013260:Steroids, Heterocyclic / Q000494:pharmacology*; D000099188:Alternative Oxidase
chemical_list: D060406:Brassinosteroids; D004075:Diglycerides; D024101:Mitochondrial Proteins; D010088:Oxidoreductases; D010712:Phosphatidic Acids; D010940:Plant Proteins; D013260:Steroids, Heterocyclic; D000099188:Alternative Oxidase; C023623:brassinolide
keywords: Alternative oxidase; Brassica napus; Brassinosteroids; Diacylglycerol; Salinity; Stomata cells