title: High relative deoxyribonucleic acid content of trophectoderm biopsy adversely affects pregnancy outcomes.
abstract: To evaluate the association between relative DNA content of the trophectoderm biopsy and pregnancy outcomes.
  
  
  
  Retrospective cohort study.
  
  
  
  Academic-affiliated private practice.
  
  
  
  This study included patients undergoing their first single embryo transfer after trophectoderm biopsy and comprehensive chromosome screening (CCS) at a single center between January 2010 and February 2014.
  
  
  
  In phase 1 of the study, a standard curve was developed to estimate the relative DNA content of trophectoderm biopsies. Phase 2 of the study examined reproductive outcomes in patients undergoing single embryo transfer after trophectoderm biopsy and CCS. Samples were divided into quartiles according to their relative DNA content, and clinical outcomes were compared.
  
  
  
  Chemical pregnancy rate, clinical implantation rate, ongoing pregnancy rate, live birth rate.
  
  
  
  The quartile of highest relative DNA content had a significantly lower live birth rate when compared with the other three quartiles (relative risk 0.84, 95% confidence interval 0.75-0.95). There was no difference between the quartiles regarding age, body mass index, ovarian response, or endometrial thickness. Among those patients who had a live birth, there was no difference in hCG levels, gestational age at delivery, or birth weight with respect to biopsy DNA content.
  
  
  
  Trophectoderm biopsies with the highest relative DNA content are associated with lower live birth rates after single embryo transfer. Possible explanations for this phenomenon include diminished accuracy of the euploid diagnosis vs. a mechanical impact of the biopsy. Regardless of the cause, the outcomes emphasize the importance of obtaining appropriately sized trophectoderm biopsies for CCS.
authors.affiliation: Reproductive Medicine Associates of New Jersey, Basking Ridge, New Jersey; Sidney Kimmel College of Medicine, Thomas Jefferson University, Philadelphia, Pennsylvania. Electronic address: sneal@rmanj.com.
authors.forename: Shelby A
authors.initials: SA
authors.lastname: Neal
authors.affiliation: Reproductive Medicine Associates of New Jersey, Basking Ridge, New Jersey; Sidney Kimmel College of Medicine, Thomas Jefferson University, Philadelphia, Pennsylvania.
authors.forename: Jason M
authors.initials: JM
authors.lastname: Franasiak
authors.affiliation: Reproductive Medicine Associates of New Jersey, Basking Ridge, New Jersey; Sidney Kimmel College of Medicine, Thomas Jefferson University, Philadelphia, Pennsylvania.
authors.forename: Eric J
authors.initials: EJ
authors.lastname: Forman
authors.affiliation: Reproductive Medicine Associates of New Jersey, Basking Ridge, New Jersey; Sidney Kimmel College of Medicine, Thomas Jefferson University, Philadelphia, Pennsylvania.
authors.forename: Marie D
authors.initials: MD
authors.lastname: Werner
authors.affiliation: Reproductive Medicine Associates of New Jersey, Basking Ridge, New Jersey; Sidney Kimmel College of Medicine, Thomas Jefferson University, Philadelphia, Pennsylvania.
authors.forename: Scott J
authors.initials: SJ
authors.lastname: Morin
authors.affiliation: Reproductive Medicine Associates of New Jersey, Basking Ridge, New Jersey.
authors.forename: Xin
authors.initials: X
authors.lastname: Tao
authors.affiliation: Reproductive Medicine Associates of New Jersey, Basking Ridge, New Jersey; Sidney Kimmel College of Medicine, Thomas Jefferson University, Philadelphia, Pennsylvania.
authors.forename: Nathan R
authors.initials: NR
authors.lastname: Treff
authors.affiliation: Reproductive Medicine Associates of New Jersey, Basking Ridge, New Jersey; Sidney Kimmel College of Medicine, Thomas Jefferson University, Philadelphia, Pennsylvania.
authors.forename: Richard T
authors.initials: RT
authors.lastname: Scott
mesh_terms: D000328:Adult; D001706:Biopsy; D001755:Blastocyst / Q000737:chemistry* / Q000473:pathology; D004247:DNA / Q000032:analysis*; D010064:Embryo Implantation; D005260:Female; D005298:Fertility; D005307:Fertilization in Vitro / Q000009:adverse effects; D005819:Genetic Markers; D005820:Genetic Testing; D006801:Humans; D007246:Infertility / Q000175:diagnosis / Q000503:physiopathology / Q000628:therapy*; D050498:Live Birth; D008875:Middle Aged; D011237:Predictive Value of Tests; D011247:Pregnancy; D018873:Pregnancy Rate; D019836:Preimplantation Diagnosis / Q000379:methods; D012189:Retrospective Studies; D012307:Risk Factors; D056826:Single Embryo Transfer; D016896:Treatment Outcome; D015854:Up-Regulation; D055815:Young Adult
chemical_list: D005819:Genetic Markers; D004247:DNA
keywords: Comprehensive chromosome screening; IVF; preimplantation genetic diagnosis; trophectoderm biopsy