title: Oxidative Stress Induces Mouse Follicular Granulosa Cells Apoptosis via JNK/FoxO1 Pathway.
abstract: The c-Jun N-terminal protein kinase (JNK) plays an important role in the regulation of cell apoptosis. Forkhead box O (FoxO) transcription factors are involved in diverse biological processes, including cellular metabolism, cell apoptosis, and cell cycle. However, the JNK/FoxO1 pathway involved in the process of apoptosis induced by oxidative stress remains to be elucidated. Here, we demonstrated that the JNK activity significantly increased in response to oxidative stress in mouse follicular granulosa cells (MGCs). SP600125, a selective JNK inhibitor, attenuated the oxidative stress-induced MGCs apoptosis. Oxidative stress enhanced the FoxO1 nuclear translocation by activating the JNK activity. Moreover, JNK mediated the dissociation of FoxO1 from 14-3-3 proteins in MGCs after the treatment with H2O2. Finally, oxidative stress up-regulated the expression of FoxO1 via JNK mediation of FoxO1 self-regulation in MGCs. Taken together, our findings suggest that JNK/FoxO1 is involved in the regulation of oxidative stress-induced cell apoptosis in MGCs.
authors.affiliation: College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, China.
authors.forename: Qiannan
authors.initials: Q
authors.lastname: Weng
authors.affiliation: College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, China.|Liaoning Province of Animal Product Safety Supervision, Shenyang, China.
authors.forename: Zequn
authors.initials: Z
authors.lastname: Liu
authors.affiliation: College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, China.
authors.forename: Bojiang
authors.initials: B
authors.lastname: Li
authors.affiliation: College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, China.
authors.forename: Kaiqing
authors.initials: K
authors.lastname: Liu
authors.affiliation: College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, China.
authors.forename: Wangjun
authors.initials: W
authors.lastname: Wu
authors.affiliation: College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, China.
authors.forename: Honglin
authors.initials: H
authors.lastname: Liu
mesh_terms: D000818:Animals; D017209:Apoptosis; D005260:Female; D000071161:Forkhead Box Protein O1 / Q000378:metabolism*; D006107:Granulosa Cells / Q000166:cytology*; D006801:Humans; D048670:MAP Kinase Kinase 4 / Q000378:metabolism*; D051379:Mice; D018384:Oxidative Stress
chemical_list: D000071161:Forkhead Box Protein O1; C467200:Foxo1 protein, mouse; D048670:MAP Kinase Kinase 4