title: Chitosan nanoparticles plus quercetin suppress the oxidative stress, modulate DNA fragmentation and gene expression in the kidney of rats fed ochratoxin A-contaminated diet.
abstract: This study aimed to evaluate the protective role of chitosan nanoparticles (COS-NPs) singly or plus quercetin (Q) against OTA-induced oxidative stress and renal genotoxicity. Twelve groups of male Sprague-Dawley rats were treated orally for 3 weeks included the control group, animals fed OTA-contaminated diet (3 mg/kg diet); COS-NPs-treated groups at low (140 mg/kg b.w.) or high (280 mg/kg b.w.) dose, Q-treated group (50 mg/kg b.w.), Q plus low or high dose of COS-NPs-treated groups and OTA plus Q and/or COS-NPs at the two tested doses-treated groups. The results indicated that COS-NPs were roughly rod in shape with average particle size of 200 nm and zeta potential 31.4 ± 2.8 mV. Animals fed OTA-contaminated diet showed significant changes in serum biochemical parameters, increase kidney MDA and DNA fragmentation and decreased GPx and SOD gene expression accompanied with severe histological changes. Q and/or COS-NPs at the two tested doses induced significant improvements in all tested parameters and succeeded to overcome these effects especially in the animals treated with Q plus the high dose of COS-NPs. It could be concluded that COS-NPs are promise candidate to enhance the antioxidant effect of Q and protect against the nephrotoxicity of OTA in high endemic areas.
authors.affiliation: Food Toxicology & Contaminants Dept., National Research Center, Dokki, Cairo, Egypt. Electronic address: mosaad_abdelwahhab@yahoo.com.
authors.forename: Mosaad A
authors.initials: MA
authors.lastname: Abdel-Wahhab
authors.affiliation: Laboratory of Nutrition and Toxicology (NUTox), INSERM UMR 866, Bourgogne University, 1 Esplanade Erasme, 21000 Dijon, France.
authors.forename: Abdulhadi
authors.initials: A
authors.lastname: Aljawish
authors.affiliation: Food Toxicology & Contaminants Dept., National Research Center, Dokki, Cairo, Egypt.
authors.forename: Aziza A
authors.initials: AA
authors.lastname: El-Nekeety
authors.affiliation: Cell Biology Dept., National Research Center, Dokki, Cairo, Egypt.
authors.forename: Sekena H
authors.initials: SH
authors.lastname: Abdel-Aziem
authors.affiliation: Pathology Dept., National Research Center, Dokki, Cairo, Egypt.
authors.forename: Nabila S
authors.initials: NS
authors.lastname: Hassan
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D002273:Carcinogens / Q000633:toxicity; D048271:Chitosan / Q000494:pharmacology*; D053938:DNA Fragmentation / Q000187:drug effects*; D004032:Diet / Q000009:adverse effects*; D004338:Drug Combinations; D005506:Food Contamination / Q000032:analysis; D007668:Kidney / Q000187:drug effects*; D008099:Liver / Q000187:drug effects; D008297:Male; D008315:Malondialdehyde; D053758:Nanoparticles / Q000008:administration & dosage*; D009793:Ochratoxins / Q000633:toxicity*; D018384:Oxidative Stress / Q000187:drug effects*; D020011:Protective Agents / Q000494:pharmacology; D011794:Quercetin / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D000975:Antioxidants; D002273:Carcinogens; D004338:Drug Combinations; D009793:Ochratoxins; D020011:Protective Agents; C025589:ochratoxin A; D008315:Malondialdehyde; D048271:Chitosan; D011794:Quercetin
keywords: Chitosan nanoparticles; Mycotoxins; Nephrotoxicity; Ochratoxin A; Oxidative stress; Quercetin