title: Global protein expression analysis of molecular markers of DS-1-47, a component of implantation-promoting traditional chinese medicine.
abstract: This study investigated the molecular markers of DS-1-47, a component of an implantation- promoting traditional Chinese medicine consisting of Astragalus mongholicus, Atractylodes macrocephala, Scutellaria baicalensis and Dipsacales, in an attempt to clarify the molecular mechanism and action targets of DS-1-47. Controlled ovarian stimulation (COS) method was used to establish the implantation dysfunction models of mice. Animals were divided into normal pregnant group, COS model group and DS-1-47 group. Laser capture microdissection-double dimensional electrophoresis-mass spectrum (LCM-DE-MS) was used to analyze the uterine protein molecules that were possibly involved in the promotion of implantation. Twenty-three proteins in DS-1-47 group were significantly changed as compared to those in COS model group, with 7 proteins down-regulated and 16 proteins up-regulated. Except for some constituent proteins, the down-regulated proteins included collagen α-1 (VI) chain, keratin 7, keratin 14, myosin regulatory light chain 12B, myosin light polypeptide 9, heat shock protein β-7, and C-U-editing enzyme APOBEC-2; the up-regulated proteins included apolipoprotein A-I, calcium regulated protein-3, proliferating cell nuclear antigen, L-xylulose reductase, and calcium binding protein. These 23 proteins that were regulated by DS-1-47 represented a broad diversity of molecule functions. The down-regulated proteins were associated with stress and immune response, and those up-regulated proteins were related to proliferation. It was suggested that these proteins were important in regulating the uterine environment for the blastocyst implantation. By identification of DS-1-47 markers, proteomic analysis coupled with functional assays is demonstrated to be a promising approach to better understand the molecular mechanism of traditional Chinese medicine.
authors.affiliation: Department of Traditional Chinese Medicine, School of Pharmacy, Tongji Medical School, Huazhong University of Science and Technology, Wuhan, 430030, China.
authors.forename: Yan-Ling
authors.initials: YL
authors.lastname: Li
authors.affiliation: Department of Traditional Chinese Medicine, School of Pharmacy, Tongji Medical School, Huazhong University of Science and Technology, Wuhan, 430030, China.
authors.forename: Xiao-Yan
authors.initials: XY
authors.lastname: Zhang
authors.affiliation: Department of Traditional Chinese Medicine, School of Pharmacy, Tongji Medical School, Huazhong University of Science and Technology, Wuhan, 430030, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Leng
authors.affiliation: Department of Traditional Chinese Medicine, School of Pharmacy, Tongji Medical School, Huazhong University of Science and Technology, Wuhan, 430030, China.
authors.forename: Yan-Li
authors.initials: YL
authors.lastname: Wu
authors.affiliation: Department of Emergency, Hubei General Hospital of Chinese People's Armed Police Forces, Wuhan, 430061, China. 1150790777@qq.com.
authors.forename: Jing
authors.initials: J
authors.lastname: Li
authors.affiliation: Department of Traditional Chinese Medicine, School of Pharmacy, Tongji Medical School, Huazhong University of Science and Technology, Wuhan, 430030, China. wuyunxia@hust.edu.cn.
authors.forename: Yun-Xia
authors.initials: YX
authors.lastname: Wu
mesh_terms: D000818:Animals; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D010064:Embryo Implantation / Q000187:drug effects*; D005260:Female; D051379:Mice; D010062:Ovulation Induction; D011247:Pregnancy; D020543:Proteome / Q000235:genetics / Q000378:metabolism*; D014599:Uterus / Q000187:drug effects / Q000378:metabolism / Q000502:physiology
chemical_list: C000618183:DS-1-47; D004365:Drugs, Chinese Herbal; D020543:Proteome
keywords: DS-1-47; LCM-DE-MS; promoting implantation; proteomics