title: Versatile wetting measurement of microplate wells.
abstract: A method to measure the contact angle, which is indicative of wetting, using small liquid volumes dispensed directly on microplate wells is described and demonstrated. Experiments with enhanced green protein samples of volumes 4.4-6 μl showed no measured variance in the contact angle. Experiments with phosphate buffer solution with varied concentrations of a non-ionic detergent (Tween 20) dissolved, however, revealed smaller contact angles with increased detergent concentration. It is experimentally shown that drops can be located up to 7° from the lowest position of the well without affecting the accuracy of contact angle measurements. Numerical simulations confirm the ability of the drops to manifest the correct contact angle despite the lack of axis-symmetry in their shape while residing on a circular surface. This method offers a convenient means to determine the wetting characteristics of different liquid samples in different microplates.
authors.affiliation: Laboratory for Optics and Applied Mechanics, Department of Mechanical and Aerospace Engineering, Monash University, Clayton VIC3800, Australia.
authors.forename: Enoch Ming Wei
authors.initials: EM
authors.lastname: Ng
authors.affiliation: Laboratory for Optics and Applied Mechanics, Department of Mechanical and Aerospace Engineering, Monash University, Clayton VIC3800, Australia.
authors.forename: Brandon Huey-Ping
authors.initials: BH
authors.lastname: Cheong
authors.affiliation: Department of Mechanics, Beijing Institute of Technology, Beijing 100081, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Yu
authors.affiliation: Yong Loo Lin School of Medicine, Centre for Translational Medicine, Cardiovascular Research Institute, National University of Singapore, National University Health System, 14 Medical Drive, Singapore 117599.
authors.forename: Oi Wah
authors.initials: OW
authors.lastname: Liew
authors.affiliation: Laboratory for Optics and Applied Mechanics, Department of Mechanical and Aerospace Engineering, Monash University, Clayton VIC3800, Australia.
authors.forename: Tuck Wah
authors.initials: TW
authors.lastname: Ng