title: MicroRNA-101b attenuates cardiomyocyte hypertrophy by inhibiting protein kinase C epsilon signaling.
abstract: Previously, a surgical regression model identified microRNA-101b (miR-101b) as a potential inhibitor of cardiac hypertrophy. Here, we investigated the antihypertrophic mechanism of miR-101b using neonatal rat ventricular myocytes. miR-101b markedly suppressed agonist-induced cardiac hypertrophy as shown by cell size and fetal gene expression. By systems biology approaches, we identified protein kinase C epsilon (PKCε) as the major target of miR-101b. Our results from qRT-PCR, western blot, and luciferase reporter assays confirm that PKCε is a direct target of miR-101b. In addition, we found that effectors downstream of PKCε (p-AKT, p-ERK1/2, p-NFAT, and p-GSK3β) are also affected by miR-101b. Our study reveals a novel inhibitory mechanism for miR-101b as a negative regulator of cardiac hypertrophy.
authors.affiliation: College of Life Sciences, Gwangju Institute of Science and Technology, Korea.
authors.forename: Jong Sub
authors.initials: JS
authors.lastname: Lee
authors.affiliation: College of Life Sciences, Gwangju Institute of Science and Technology, Korea.
authors.forename: Dong Kwon
authors.initials: DK
authors.lastname: Yang
authors.affiliation: College of Life Sciences, Gwangju Institute of Science and Technology, Korea.
authors.forename: Jei Hyoung
authors.initials: JH
authors.lastname: Park
authors.affiliation: College of Life Sciences, Gwangju Institute of Science and Technology, Korea.
authors.forename: Jin Ock
authors.initials: JO
authors.lastname: Kim
authors.affiliation: College of Life Sciences, Gwangju Institute of Science and Technology, Korea.
authors.forename: Woo Jin
authors.initials: WJ
authors.lastname: Park
authors.affiliation: College of Life Sciences, Gwangju Institute of Science and Technology, Korea.
authors.forename: Chunghee
authors.initials: C
authors.lastname: Cho
authors.affiliation: College of Life Sciences, Gwangju Institute of Science and Technology, Korea.
authors.forename: Do Han
authors.initials: DH
authors.lastname: Kim
mesh_terms: D000818:Animals; D001483:Base Sequence; D006332:Cardiomegaly / Q000201:enzymology* / Q000235:genetics / Q000473:pathology*; D019332:Endothelin-1 / Q000494:pharmacology; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D032383:Myocytes, Cardiac / Q000187:drug effects / Q000201:enzymology* / Q000378:metabolism / Q000473:pathology*; D051744:Protein Kinase C-epsilon / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D017207:Rats, Sprague-Dawley; D015398:Signal Transduction / Q000187:drug effects
chemical_list: D019332:Endothelin-1; C579142:MIRN101 microRNA, rat; D035683:MicroRNAs; D012333:RNA, Messenger; D051744:Protein Kinase C-epsilon
keywords: 
  miR-101b
  ; cardiomyocytes; microRNA; myocardial hypertrophy; protein kinase C; systems biology