title: Thyroid hormone status defines brown adipose tissue activity and browning of white adipose tissues in mice.
abstract: The present study aimed to determine the effect of thyroid hormone dysfunction on brown adipose tissue activity and white adipose tissue browning in mice. Twenty randomized female C57BL/6NTac mice per treatment group housed at room temperature were rendered hypothyroid or hyperthyroid. In-vivo small animal18F-FDG PET/MRI was performed to determine the effects of hypo- and hyperthyroidism on BAT mass and BAT activity. Ex-vivo14C-acetate loading assay and assessment of thermogenic gene and protein expression permitted analysis of oxidative and thermogenic capacities of WAT and BAT of eu-, hyper and hypothyroid mice.18F-FDG PET/MRI revealed a lack of brown adipose tissue activity in hypothyroid mice, whereas hyperthyroid mice displayed increased BAT mass alongside enhanced18F-FDG uptake. In white adipose tissue of both, hyper- and hypothyroid mice, we found a significant induction of thermogenic genes together with multilocular adipocytes expressing UCP1. Taken together, these results suggest that both the hyperthyroid and hypothyroid state stimulate WAT thermogenesis most likely as a consequence of enhanced adrenergic signaling or compensation for impaired BAT function, respectively.
authors.affiliation: Department of Endocrinology and Nephrology, University Hospital, Leipzig, Germany.
authors.forename: Juliane
authors.initials: J
authors.lastname: Weiner
authors.affiliation: Helmholtz-Zentrum Dresden-Rossendorf, Institute of Radiopharmaceutical Cancer Research, Research Site Leipzig, Department of Neuroradiopharmaceuticals, Leipzig, Germany.
authors.forename: Mathias
authors.initials: M
authors.lastname: Kranz
authors.affiliation: Department of Endocrinology and Nephrology, University Hospital, Leipzig, Germany.|University of Leipzig, IFB Adiposity Diseases, Leipzig, Germany.
authors.forename: Nora
authors.initials: N
authors.lastname: Klöting
authors.affiliation: German Center for Diabetes Research (DZD), Leipzig, Germany.
authors.forename: Anne
authors.initials: A
authors.lastname: Kunath
authors.affiliation: Department of Nuclear Medicine, University Hospital, Leipzig, Germany.
authors.forename: Karen
authors.initials: K
authors.lastname: Steinhoff
authors.affiliation: Institute of Experimental Endocrinology, Charité University Hospital, Berlin, Germany.
authors.forename: Eddy
authors.initials: E
authors.lastname: Rijntjes
authors.affiliation: Institute of Experimental Endocrinology, Charité University Hospital, Berlin, Germany.
authors.forename: Josef
authors.initials: J
authors.lastname: Köhrle
authors.affiliation: Department of Nuclear Medicine, University Hospital, Leipzig, Germany.
authors.forename: Vilia
authors.initials: V
authors.lastname: Zeisig
authors.affiliation: University of Leipzig, IFB Adiposity Diseases, Leipzig, Germany.
authors.forename: Mohammed
authors.initials: M
authors.lastname: Hankir
authors.affiliation: Department of Endocrinology and Nephrology, University Hospital, Leipzig, Germany.
authors.forename: Claudia
authors.initials: C
authors.lastname: Gebhardt
authors.affiliation: Helmholtz-Zentrum Dresden-Rossendorf, Institute of Radiopharmaceutical Cancer Research, Research Site Leipzig, Department of Neuroradiopharmaceuticals, Leipzig, Germany.
authors.forename: Winnie
authors.initials: W
authors.lastname: Deuther-Conrad
authors.affiliation: Department of Endocrinology and Nephrology, University Hospital, Leipzig, Germany.
authors.forename: John T
authors.initials: JT
authors.lastname: Heiker
authors.affiliation: Department of Endocrinology and Nephrology, University Hospital, Leipzig, Germany.|University of Leipzig, IFB Adiposity Diseases, Leipzig, Germany.
authors.forename: Susan
authors.initials: S
authors.lastname: Kralisch
authors.affiliation: Department of Endocrinology and Nephrology, University Hospital, Leipzig, Germany.|University of Leipzig, IFB Adiposity Diseases, Leipzig, Germany.
authors.forename: Michael
authors.initials: M
authors.lastname: Stumvoll
authors.affiliation: Department of Endocrinology and Nephrology, University Hospital, Leipzig, Germany.|University of Leipzig, IFB Adiposity Diseases, Leipzig, Germany.
authors.forename: Matthias
authors.initials: M
authors.lastname: Blüher
authors.affiliation: University of Leipzig, IFB Adiposity Diseases, Leipzig, Germany.|Department of Nuclear Medicine, University Hospital, Leipzig, Germany.
authors.forename: Osama
authors.initials: O
authors.lastname: Sabri
authors.affiliation: University of Leipzig, IFB Adiposity Diseases, Leipzig, Germany.|Department of Nuclear Medicine, University Hospital, Leipzig, Germany.
authors.forename: Swen
authors.initials: S
authors.lastname: Hesse
authors.affiliation: Helmholtz-Zentrum Dresden-Rossendorf, Institute of Radiopharmaceutical Cancer Research, Research Site Leipzig, Department of Neuroradiopharmaceuticals, Leipzig, Germany.
authors.forename: Peter
authors.initials: P
authors.lastname: Brust
authors.affiliation: Department of Endocrinology and Nephrology, University Hospital, Leipzig, Germany.
authors.forename: Anke
authors.initials: A
authors.lastname: Tönjes
authors.affiliation: Department of Endocrinology and Nephrology, University Hospital, Leipzig, Germany.
authors.forename: Kerstin
authors.initials: K
authors.lastname: Krause
mesh_terms: D017667:Adipocytes; D002001:Adipose Tissue, Brown / Q000378:metabolism*; D052436:Adipose Tissue, White / Q000378:metabolism*; D000818:Animals; D005260:Female; D019788:Fluorodeoxyglucose F18 / Q000378:metabolism; D005786:Gene Expression Regulation; D006980:Hyperthyroidism / Q000139:chemically induced / Q000000981:diagnostic imaging* / Q000378:metabolism; D007037:Hypothyroidism / Q000139:chemically induced / Q000000981:diagnostic imaging* / Q000378:metabolism; D008279:Magnetic Resonance Imaging; D051379:Mice; D008810:Mice, Inbred C57BL; D011441:Propylthiouracil / Q000009:adverse effects; D011897:Random Allocation; D015398:Signal Transduction; D022722:Thermogenesis; D013974:Thyroxine / Q000009:adverse effects
chemical_list: D019788:Fluorodeoxyglucose F18; D011441:Propylthiouracil; D013974:Thyroxine