title: Biodegradable Magnetic Silica@Iron Oxide Nanovectors with Ultra-Large Mesopores for High Protein Loading, Magnetothermal Release, and Delivery.
abstract: The delivery of large cargos of diameter above 15nm for biomedical applications has proved challenging since it requires biocompatible, stably-loaded, and biodegradable nanomaterials. In this study, we describe the design of biodegradable silica-iron oxide hybrid nanovectors with large mesopores for large protein delivery in cancer cells. The mesopores of the nanomaterials spanned from 20 to 60nm in diameter and post-functionalization allowed the electrostatic immobilization of large proteins (e.g. mTFP-Ferritin, ~534kDa). Half of the content of the nanovectors was based with iron oxide nanophases which allowed the rapid biodegradation of the carrier in fetal bovine serum and a magnetic responsiveness. The nanovectors released large protein cargos in aqueous solution under acidic pH or magnetic stimuli. The delivery of large proteins was then autonomously achieved in cancer cells via the silica-iron oxide nanovectors, which is thus a promising for biomedical applications.
authors.affiliation: Smart Hybrid Materials Laboratory, Advanced Membranes and Porous Materials Center, King Abdullah University of Science and Technology, Thuwal, Saudi Arabia.
authors.forename: Haneen
authors.initials: H
authors.lastname: Omar
authors.affiliation: Smart Hybrid Materials Laboratory, Advanced Membranes and Porous Materials Center, King Abdullah University of Science and Technology, Thuwal, Saudi Arabia.
authors.forename: Jonas G
authors.initials: JG
authors.lastname: Croissant
authors.affiliation: Smart Hybrid Materials Laboratory, Advanced Membranes and Porous Materials Center, King Abdullah University of Science and Technology, Thuwal, Saudi Arabia.
authors.forename: Kholod
authors.initials: K
authors.lastname: Alamoudi
authors.affiliation: Smart Hybrid Materials Laboratory, Advanced Membranes and Porous Materials Center, King Abdullah University of Science and Technology, Thuwal, Saudi Arabia.
authors.forename: Shahad
authors.initials: S
authors.lastname: Alsaiari
authors.affiliation: Life sciences and Environment Research Institute, Center of Excellence in Nanomedicine (CENM), King Abdulaziz City for Science and Technology (KACST), Riyadh 11461, Saudi Arabia.
authors.forename: Ibrahim
authors.initials: I
authors.lastname: Alradwan
authors.affiliation: Life sciences and Environment Research Institute, Center of Excellence in Nanomedicine (CENM), King Abdulaziz City for Science and Technology (KACST), Riyadh 11461, Saudi Arabia.
authors.forename: Majed A
authors.initials: MA
authors.lastname: Majrashi
authors.affiliation: Imaging and Characterization Laboratory, King Abdullah University of Science and Technology, Thuwal, Saudi Arabia.
authors.forename: Dalaver H
authors.initials: DH
authors.lastname: Anjum
authors.affiliation: Smart Hybrid Materials Laboratory, Advanced Membranes and Porous Materials Center, King Abdullah University of Science and Technology, Thuwal, Saudi Arabia.
authors.forename: Patricia
authors.initials: P
authors.lastname: Martins
authors.affiliation: KAUST Catalysis Center (KCC), Physical Science and Engineering Division, King Abdullah University of Science and Technology (KAUST).
authors.forename: Ria
authors.initials: R
authors.lastname: Laamarti
authors.affiliation: KAUST Catalysis Center (KCC), Physical Science and Engineering Division, King Abdullah University of Science and Technology (KAUST).
authors.forename: Jorg
authors.initials: J
authors.lastname: Eppinger
authors.affiliation: Smart Hybrid Materials Laboratory, Advanced Membranes and Porous Materials Center, King Abdullah University of Science and Technology, Thuwal, Saudi Arabia.
authors.forename: Basem
authors.initials: B
authors.lastname: Moosa
authors.affiliation: Life sciences and Environment Research Institute, Center of Excellence in Nanomedicine (CENM), King Abdulaziz City for Science and Technology (KACST), Riyadh 11461, Saudi Arabia. Electronic address: aalmalik@kacst.edu.sa.
authors.forename: Abdulaziz
authors.initials: A
authors.lastname: Almalik
authors.affiliation: Smart Hybrid Materials Laboratory, Advanced Membranes and Porous Materials Center, King Abdullah University of Science and Technology, Thuwal, Saudi Arabia.. Electronic address: niveen.khashab@kaust.edu.sa.
authors.forename: Niveen M
authors.initials: NM
authors.lastname: Khashab
mesh_terms: D016503:Drug Delivery Systems; D005290:Ferric Compounds / Q000008:administration & dosage / Q000737:chemistry; D005293:Ferritins / Q000008:administration & dosage / Q000737:chemistry; D005456:Fluorescent Dyes / Q000008:administration & dosage / Q000737:chemistry; D049452:Green Fluorescent Proteins / Q000008:administration & dosage / Q000737:chemistry; D006367:HeLa Cells; D006801:Humans; D060328:Magnetic Phenomena; D053761:Nanocomposites / Q000008:administration & dosage / Q000737:chemistry; D053758:Nanoparticles / Q000008:administration & dosage / Q000737:chemistry; D016062:Porosity; D011437:Propylamines / Q000008:administration & dosage / Q000737:chemistry; D012822:Silicon Dioxide / Q000008:administration & dosage / Q000737:chemistry
chemical_list: C485182:Cyan Fluorescent Protein; D005290:Ferric Compounds; D005456:Fluorescent Dyes; D011437:Propylamines; D049452:Green Fluorescent Proteins; C000499:ferric oxide; D012822:Silicon Dioxide; D005293:Ferritins
keywords: biodegradable nanoparticles; magnetic nanoparticles; pH triggered delivery; protein delivery; silica nanoparticles