title: Acthaside: a new chromone derivative from Acacia ataxacantha and its biological activities.
abstract: BACKGROUND
  Acacia ataxacantha (Fabaceae), used in traditional medicine grows in the South-West of Bénin. Ethyl acetate extract of the barks of this species was previously reported to display various bioactivities, including antibacterial, antifungal and antioxidant activities. In the present study, we investigate the antimicrobial and antioxidant activities of compound isolated from ethyl acetate extract of Acacia ataxacantha.
  
  
  METHODS
  Purification, isolation and structural identification of isolated compound were done using various chromatographic and spectroscopic methods. Antimicrobial activity was investigated using a two-fold serial microdilution method. The inhibitory potency of isolated compound was evaluated by kinetic experiments. The antioxidant activity was also determined using 2, 2-diphenyl-1-picrylhydrazyl.
  
  
  RESULTS
  The isolated compound was identified as 7-hydroxy-2-methyl-6-[β-galactopyranosyl-propyl]-4H-chromen-4-one. As far as we know, this compound, named "acthaside", reported for the first time, was active against all tested microorganisms with minimal inhibitory concentration ranging from 25 to 50 μg/ml. At 50 μl/ml, no growth was observed in almost all tested microbial after 24 h of exposure. The isolated compound had significant antioxidant activity with an IC50value of 3.61 ± 0.12 μg/ml compared to quercetin (IC501.04 ± 0.01 μg/ml).
  
  
  CONCLUSIONS
  The present work demonstrates that the new chromen derivative isolated from A. ataxacantha may help treat bacterial and yeast infections. However, further studies are required to clarify the mechanism of action of this compound.
authors.affiliation: Unité de Biochimie et Biologie Moléculaire, Equipe de Biochimie et Substances Naturelles Bioactives, Faculté des Sciences et Techniques, Université d'Abomey-Calavi, 04 BP 0320, Cotonou, Benin.
authors.forename: Abdou Madjid O
authors.initials: AM
authors.lastname: Amoussa
authors.affiliation: Laboratoire d'Innovation Thérapeutique, Faculté de Pharmacie, UMR CNRS-Unistra 7200, 74 route du Rhin, CS 60024, 67401, Illkich, France.
authors.forename: Mélanie
authors.initials: M
authors.lastname: Bourjot
authors.affiliation: Unité de Biochimie et Biologie Moléculaire, Equipe de Biochimie et Substances Naturelles Bioactives, Faculté des Sciences et Techniques, Université d'Abomey-Calavi, 04 BP 0320, Cotonou, Benin. llagnika@gmail.com.
authors.forename: Latifou
authors.initials: L
authors.lastname: Lagnika
authors.affiliation: Laboratoire d'Innovation Thérapeutique, Faculté de Pharmacie, UMR CNRS-Unistra 7200, 74 route du Rhin, CS 60024, 67401, Illkich, France.
authors.forename: Catherine
authors.initials: C
authors.lastname: Vonthron-Sénécheau
authors.affiliation: Unité de Biochimie et Biologie Moléculaire, Equipe de Biochimie et Substances Naturelles Bioactives, Faculté des Sciences et Techniques, Université d'Abomey-Calavi, 04 BP 0320, Cotonou, Benin.
authors.forename: Ambaliou
authors.initials: A
authors.lastname: Sanni
mesh_terms: D000045:Acacia / Q000737:chemistry*; D000890:Anti-Infective Agents / Q000302:isolation & purification*; D000975:Antioxidants / Q000302:isolation & purification*; D002867:Chromones / Q000302:isolation & purification*; D005697:Galactosides / Q000302:isolation & purification*; D008826:Microbial Sensitivity Tests; D015394:Molecular Structure; D024301:Plant Bark / Q000737:chemistry
chemical_list: D000890:Anti-Infective Agents; D000975:Antioxidants; D002867:Chromones; D005697:Galactosides; C000613734:acthaside
keywords: 7-hydroxy-2-methyl-6-[β-galactopyranosyl-propyl]-4H-Chromen-4-one (acthaside); Acacia ataxacantha; Antimicrobial; Antioxidant