title: The combined effect of resveratrol and diphenyleneiodonium on irradiation-induced injury to the hematopoietic system.
abstract: Both resveratrol(Res) and diphenyleneiodonium(DPI) have been shown to have radioprotective effects on hematopoietic system injury. However, the cooperative effect of Res and DPI are unknown. In this study, we explored the radioprotective effect of the combination of Res and DPI both in vitro and in vivo. Our results showed that the combined treatment of Res and DPI was more effective in protecting irradiated BMMNCs in terms of cell viability, colony-forming ability, and reconstitution ability in vitro compared with Res or DPI treatment alone. However, in mice, the combination of Res and DPI had no enhanced protection on 4Gy total body irradiation (TBI)-induced hematopoietic system injury, including TBI-induced myelosuppression, induction of the splenic index, and increases in HSC/HPC numbers and the colony-forming ability of BMCs,compared to Res or DPI alone. An exception was the number of BMCs. These studies illustrated the inconsistency between experiments carried out in vitro and in vivo and suggest an interaction between Res or DPI in vivo.
authors.affiliation: Tianjin Key Lab of Radiation Medicine and Molecular Nuclear Medicine, Institute of Radiation Medicine, Peking Union Medical College, Chinese Academy of Medical Science, Tianjin 300192, China.
authors.forename: Junling
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Tianjin Key Lab of Radiation Medicine and Molecular Nuclear Medicine, Institute of Radiation Medicine, Peking Union Medical College, Chinese Academy of Medical Science, Tianjin 300192, China.
authors.forename: Xiaodan
authors.initials: X
authors.lastname: Han
authors.affiliation: Tianjin Key Lab of Radiation Medicine and Molecular Nuclear Medicine, Institute of Radiation Medicine, Peking Union Medical College, Chinese Academy of Medical Science, Tianjin 300192, China.
authors.forename: Song
authors.initials: S
authors.lastname: Huang
authors.affiliation: Tianjin Key Lab of Radiation Medicine and Molecular Nuclear Medicine, Institute of Radiation Medicine, Peking Union Medical College, Chinese Academy of Medical Science, Tianjin 300192, China.
authors.forename: Lu
authors.initials: L
authors.lastname: Lu
authors.affiliation: Tianjin Key Lab of Radiation Medicine and Molecular Nuclear Medicine, Institute of Radiation Medicine, Peking Union Medical College, Chinese Academy of Medical Science, Tianjin 300192, China.
authors.forename: Deguan
authors.initials: D
authors.lastname: Li
authors.affiliation: Tianjin Key Lab of Radiation Medicine and Molecular Nuclear Medicine, Institute of Radiation Medicine, Peking Union Medical College, Chinese Academy of Medical Science, Tianjin 300192, China; Institute of Laboratory Animal Science, Peking Union Medical College, Chinese Academy of Medical Science, Beijing 100021, China. Electronic address: ai_min_meng@126.com.
authors.forename: Aimin
authors.initials: A
authors.lastname: Meng
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000187:drug effects* / Q000528:radiation effects; D002478:Cells, Cultured; D004347:Drug Interactions; D004359:Drug Therapy, Combination; D006413:Hematopoietic System / Q000187:drug effects* / Q000528:radiation effects; D006801:Humans; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009861:Onium Compounds / Q000627:therapeutic use*; D011833:Radiation Injuries, Experimental / Q000188:drug therapy*; D000077185:Resveratrol; D013267:Stilbenes / Q000627:therapeutic use*; D014916:Whole-Body Irradiation
chemical_list: D009861:Onium Compounds; D013267:Stilbenes; C007517:diphenyleneiodonium; D000077185:Resveratrol
keywords: Combination; Diphenyleneiodonium; Radiation;hematopoietic system; Resveratrol