title: A Simple Method for Ion Channel Recordings Using Fine Gold Electrode.
abstract: The artificial bilayer single-channel recording technique is commonly used to observe detailed pharmacological properties of various ion channel proteins. It permits easy control of the solution and membrane lipid composition, and is also compatible with pharmacological screening devices. However, its use is limited due to low measurement efficiency. Here, we develop a novel artificial bilayer single-channel recording technique in which bilayers are made and channels are reconstituted into the membranes by contacting a gold electrode to the lipid-solution interface. Using this technique, we measured the single-channel currents of two channel-forming peptides, gramicidin and alamethicin, and a channel-forming protein, α-hemolysin. This technique requires only one action, allowing the technique to potentially be combined with high-throughput screening devices.
authors.affiliation: Laboratory for Cell Dynamics Observation, Quantitative Biology Center, Riken.
authors.forename: Daichi
authors.initials: D
authors.lastname: Okuno
authors.forename: Minako
authors.initials: M
authors.lastname: Hirano
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Yokota
authors.forename: Yukiko
authors.initials: Y
authors.lastname: Onishi
authors.forename: Junya
authors.initials: J
authors.lastname: Ichinose
authors.forename: Toru
authors.initials: T
authors.lastname: Ide
mesh_terms: D002623:Chemistry Techniques, Analytical / Q000295:instrumentation* / Q000379:methods*; D004566:Electrodes; D006046:Gold / Q000737:chemistry*; D006096:Gramicidin / Q000737:chemistry; D015640:Ion Channel Gating; D008051:Lipid Bilayers / Q000737:chemistry; D015203:Reproducibility of Results
chemical_list: D008051:Lipid Bilayers; D006096:Gramicidin; D006046:Gold