title: Generation of β-lactoglobulin-modified transgenic goats by homologous recombination.
abstract: β-Lactoglobulin (BLG) is a dominant allergen present in the milk of goats and other ungulates, although it is not found in human breast milk. Thus, the presence of BLG restricts the consumption of goat's milk by humans. In the present study, we examined whether the disruption of the BLG gene in goats by homologous recombination (HR) reduced BLG content in goat's milk and decreased the allergic response to milk. In one approach, exon 2 of the BLG gene was efficiently targeted using HR with a BLG knockout vector. In a second approach to disrupt BLG gene expression and drive exogenous human α-lactalbumin (hLA) gene expression, two hLA knock-in constructs were used to target exons 1-4 of the BLG gene via HR, and expression of hLA was then confirmed in goat mammary epithelial cells in vitro. The recombinant clones from both approaches were then used for somatic cell nuclear transfer, generating two transgenic goats possessing a BLG knockout allele or site-specific hLA integration allele. Milk assays demonstrated a reduction in BLG levels in both the BLG knockout and hLA knock-in goats; furthermore, hLA was present in the hLA knock-in goat's milk. Allergenic analysis in mice indicated that the transgenic goat's milk was less allergenic than wild-type goat's milk. These results support the development of gene-targeted animals as an effective tool for reducing allergic reactions to milk and improving nutrition.
authors.affiliation: College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, China.|Key Laboratory of Animal Biotechnology, Ministry of Agriculture, Northwest A&F University, Yangling, Shaanxi, China.
authors.forename: Hongmei
authors.initials: H
authors.lastname: Zhu
authors.affiliation: College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, China.|Key Laboratory of Adaptation and Evolution of Plateau Biota, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining, China.
authors.forename: Linyong
authors.initials: L
authors.lastname: Hu
authors.affiliation: College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, China.|Key Laboratory of Animal Biotechnology, Ministry of Agriculture, Northwest A&F University, Yangling, Shaanxi, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Liu
authors.affiliation: College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, China.|Key Laboratory of Animal Biotechnology, Ministry of Agriculture, Northwest A&F University, Yangling, Shaanxi, China.
authors.forename: Huatao
authors.identifier: 0000-0002-3800-5013
authors.initials: H
authors.lastname: Chen
authors.affiliation: College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, China.|Key Laboratory of Animal Biotechnology, Ministry of Agriculture, Northwest A&F University, Yangling, Shaanxi, China.
authors.forename: Chenchen
authors.initials: C
authors.lastname: Cui
authors.affiliation: College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, China.|Key Laboratory of Animal Biotechnology, Ministry of Agriculture, Northwest A&F University, Yangling, Shaanxi, China.
authors.forename: Yujie
authors.initials: Y
authors.lastname: Song
authors.affiliation: College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, China.|Key Laboratory of Animal Biotechnology, Ministry of Agriculture, Northwest A&F University, Yangling, Shaanxi, China.
authors.forename: Yaping
authors.initials: Y
authors.lastname: Jin
authors.affiliation: College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, China.|Key Laboratory of Animal Biotechnology, Ministry of Agriculture, Northwest A&F University, Yangling, Shaanxi, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D000485:Allergens / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D000818:Animals; D030801:Animals, Genetically Modified; D002478:Cells, Cultured; D005091:Exons / Q000235:genetics*; D005260:Female; D015870:Gene Expression; D055786:Gene Knockout Techniques / Q000379:methods; D006041:Goats; D057809:HEK293 Cells; D059765:Homologous Recombination / Q000235:genetics*; D006801:Humans; D007073:Immunoglobulin E / Q000097:blood / Q000276:immunology; D007768:Lactalbumin / Q000235:genetics / Q000378:metabolism; D007782:Lactoglobulins / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D008297:Male; D008807:Mice, Inbred BALB C; D008892:Milk / Q000009:adverse effects / Q000378:metabolism; D016269:Milk Hypersensitivity / Q000097:blood / Q000209:etiology / Q000276:immunology; D009154:Mutation; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D000485:Allergens; D007782:Lactoglobulins; D007073:Immunoglobulin E; D007768:Lactalbumin
keywords: homologous recombination; milk allergy; somatic cell nuclear transfer; transgenic goat; β-lactoglobulin