title: A Novel Fluorescent Quantum Dot Probe for the Rapid Diagnostic High Contrast Imaging of Tumor in Mice.
abstract: A simple probe - antibody conjugated silica over coated cadmium selenide quantum dots (QD-Ab probe) for efficient and rapid diagnostic in vivo imaging of tumors is developed. Compared to unconjugated quantum dots (QD), these probes underwent efficient cellular internalization and tumor targeting behavior, retaining bright emission under in vivo cancer models. Silica over coated cadmium selenide quantum dots were conjugated with Epidermal growth factor receptor (EGFR) monoclonal antibody to detect the over expression of EGFR in cancer models. The in vitro cellular internalization efficiency of QD and QD-Ab probe in cultured stem cells (RADMSCs) and cancer cells (HeLa) were assessed by ICP-OES and cLSM. Results demonstrated a greater internalization efficiency of CdSe-Silica QD-Ab probe than CdSe-Silica QDs. For in vivo imaging solid tumor bearing mice was subjected to tail vein injection of QD and QD-Ab probe. After the specific time interval of injection, mice were anesthetized and subjected into Xenogen IVIS®200 imaging system, followed by ex vivo imaging. Subsequently, ultrathin sections of tumor were imaged by using cLSM. Both in vivo and ex vivo imaging results confirmed the tumor-targeted imaging efficiency of QD-Ab probes compared to unconjugated QDs.
authors.affiliation: Department of Biochemistry, University of Kerala, Kariavattom Campus, Thiruvananthapuram, 695581, Kerala, India.|Department of Biochemistry, St. Albert's College, Mahatma Gandhi University, Ernakulam, Kochi, 682018, Kerala, India.|Department of Pharmaceutical Sciences, South Dakota State University, Brookings, SD, 57007, USA.
authors.forename: M
authors.initials: M
authors.lastname: Vibin
authors.affiliation: Department of Chemistry, SVR NSS College Vazhoor, Mahatma Gandhi University, Kottayam, 686505, Kerala, India.
authors.forename: R
authors.initials: R
authors.lastname: Vinayakan
authors.affiliation: TEM Laboratory, Sree Chitra Tirunal Institute of Medical Sciences and Technology, Biomedical Technology Wing, Thiruvananthapuram, 695012, Kerala, India.
authors.forename: F B
authors.initials: FB
authors.lastname: Fernandez
authors.affiliation: TEM Laboratory, Sree Chitra Tirunal Institute of Medical Sciences and Technology, Biomedical Technology Wing, Thiruvananthapuram, 695012, Kerala, India.
authors.forename: Annie
authors.initials: A
authors.lastname: John
authors.affiliation: Department of Biochemistry, University of Kerala, Kariavattom Campus, Thiruvananthapuram, 695581, Kerala, India. annieab2001@gmail.com.
authors.forename: Annie
authors.initials: A
authors.lastname: Abraham
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D019187:Cadmium Compounds / Q000737:chemistry*; D003952:Diagnostic Imaging / Q000379:methods*; D066246:ErbB Receptors / Q000276:immunology / Q000378:metabolism*; D005455:Fluorescent Antibody Technique; D005456:Fluorescent Dyes / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D018796:Immunoconjugates / Q000737:chemistry*; D008223:Lymphoma / Q000175:diagnosis* / Q000378:metabolism; D008297:Male; D051379:Mice; D045663:Quantum Dots; D018036:Selenium Compounds / Q000737:chemistry*; D012822:Silicon Dioxide / Q000737:chemistry
chemical_list: D000911:Antibodies, Monoclonal; D019187:Cadmium Compounds; D005456:Fluorescent Dyes; D018796:Immunoconjugates; D018036:Selenium Compounds; D012822:Silicon Dioxide; C058667:cadmium selenide; C512478:EGFR protein, human; D066246:ErbB Receptors
keywords: DLA cells; Ex vivo imaging; ICP-OES; Quantum Dots; Tumor-targeted imaging