title: Evaluating the potential of the GFAP-KLH immune-tolerizing vaccine for type 1 diabetes in mice.
abstract: Glial fibrillary acidic protein (GFAP), expressed in peri-islet Schwann cells, is a novel target for the treatment of type 1 diabetes mellitus (T1DM). We designed a GFAP immune-tolerizing vaccine that successfully suppresses hyperglycemia and enhances C peptide secretion. The GFAP vaccine significantly prevented T cell infiltration into pancreatic islets. Moreover, after GFAP vaccination, naïve T-cell differentiation shifted from a cytotoxic Th1- to a Th2-biased humoral response. These results indicate that as a novel target, GFAP reliably predicts the development of T1DM, and that the GFAP vaccine successfully delays the progression of T1DM by regulating T-cell differentiation.
authors.affiliation: Department of Geriatric and General Medicine, Osaka University Graduate School of Medicine, Japan.|Department of Physiology and Pathophysiology, School of Medicine, Xi'an Jiaotong University, China.
authors.forename: Zhengda
authors.initials: Z
authors.lastname: Pang
authors.affiliation: Department of Geriatric and General Medicine, Osaka University Graduate School of Medicine, Japan.
authors.forename: Masayoshi
authors.initials: M
authors.lastname: Higuchi
authors.affiliation: Department of Health Development and Medicine, Osaka University Graduate School of Medicine, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Koriyama
authors.affiliation: Department of Clinical Gene Therapy, Osaka University Graduate School of Medicine, Japan.
authors.forename: Shota
authors.initials: S
authors.lastname: Yoshida
authors.affiliation: Department of Health Development and Medicine, Osaka University Graduate School of Medicine, Japan.
authors.forename: Hitomi
authors.initials: H
authors.lastname: Kurinami
authors.affiliation: Department of Health Development and Medicine, Osaka University Graduate School of Medicine, Japan.
authors.forename: Munehisa
authors.initials: M
authors.lastname: Shimamura
authors.affiliation: Department of Geriatric and General Medicine, Osaka University Graduate School of Medicine, Japan.
authors.forename: Yoichi
authors.initials: Y
authors.lastname: Takami
authors.affiliation: Department of Geriatric and General Medicine, Osaka University Graduate School of Medicine, Japan.
authors.forename: Hiromi
authors.initials: H
authors.lastname: Rakugi
authors.affiliation: Department of Clinical Gene Therapy, Osaka University Graduate School of Medicine, Japan.
authors.forename: Ryuichi
authors.initials: R
authors.lastname: Morishita
authors.affiliation: Department of Health Development and Medicine, Osaka University Graduate School of Medicine, Japan.
authors.forename: Hironori
authors.initials: H
authors.lastname: Nakagami
mesh_terms: D000818:Animals; D002096:C-Peptide / Q000096:biosynthesis; D003922:Diabetes Mellitus, Type 1 / Q000276:immunology*; D005260:Female; D005904:Glial Fibrillary Acidic Protein / Q000276:immunology*; D006433:Hemocyanins / Q000276:immunology*; D007108:Immune Tolerance; D007109:Immunity; D007074:Immunoglobulin G / Q000378:metabolism; D007371:Interferon-gamma / Q000096:biosynthesis; D015847:Interleukin-4 / Q000096:biosynthesis; D016688:Mice, Inbred NOD; D010179:Pancreas / Q000473:pathology; D010641:Phenotype; D018417:Th1 Cells / Q000276:immunology; D018418:Th2 Cells / Q000276:immunology; D014611:Vaccination; D014612:Vaccines / Q000276:immunology*
chemical_list: D002096:C-Peptide; D005904:Glial Fibrillary Acidic Protein; D007074:Immunoglobulin G; D014612:Vaccines; D015847:Interleukin-4; D007371:Interferon-gamma; D006433:Hemocyanins; C032808:keyhole-limpet hemocyanin
keywords: glial fibrillary acidic protein; type 1 diabetes; vaccine