title: Immobilization Techniques of Bacteria for Live Super-resolution Imaging Using Structured Illumination Microscopy.
abstract: Advancements in optical microscopy technology have allowed huge progression in the ability to understand protein structure and dynamics in live bacterial cells using fluorescence microscopy. Paramount to high-quality microscopy is good sample preparation to avoid bacterial cell movement that can result in motion blur during image acquisition. Here, we describe two techniques of sample preparation that reduce unwanted cell movement and are suitable for application to a number of bacterial species and imaging methods.
authors.affiliation: The ithree institute, University of Technology Sydney, Cnr Thomas & Harris Street, Ultimo, Sydney, NSW, 2007, Australia.
authors.forename: Amy L
authors.initials: AL
authors.lastname: Bottomley
authors.affiliation: The ithree institute, University of Technology Sydney, Cnr Thomas & Harris Street, Ultimo, Sydney, NSW, 2007, Australia.
authors.forename: Lynne
authors.initials: L
authors.lastname: Turnbull
authors.affiliation: The ithree institute, University of Technology Sydney, Cnr Thomas & Harris Street, Ultimo, Sydney, NSW, 2007, Australia.
authors.forename: Cynthia B
authors.initials: CB
authors.lastname: Whitchurch
authors.affiliation: The ithree institute, University of Technology Sydney, Cnr Thomas & Harris Street, Ultimo, Sydney, NSW, 2007, Australia. Elizabeth.Harry@uts.edu.au.
authors.forename: Elizabeth J
authors.initials: EJ
authors.lastname: Harry
mesh_terms: D001419:Bacteria; D007091:Image Processing, Computer-Assisted; D021621:Imaging, Three-Dimensional / Q000379:methods; D008856:Microscopy, Fluorescence / Q000379:methods*
keywords: Agarose pads; Bacterial slide preparation; Fast three-dimensional structured illumination microscopy; GelGro slabs; Super-resolution microscopy