title: Moderate intake of docosahexaenoic acid raises plasma and platelet vitamin E levels in cystic fibrosis patients.
abstract: Patients with cystic fibrosis have increased oxidative stress and impaired antioxidant systems. Moderate intake of docosahexaenoic acid (DHA) may favor the lowering of oxidative stress. In this randomized, double-blind, cross-over study, DHA or placebo capsules, were given daily to 10 patients, 5mg/kg for 2 weeks then 10mg/kg DHA for the next 2 weeks (or placebo). After 9 weeks of wash-out, patients took placebo or DHA capsules. Biomarkers of lipid peroxidation and vitamin E were measured at baseline, and after 2 and 4 weeks of treatment in each phase. The proportions of DHA increased both in plasma and platelet lipids after DHA supplementations. The lipid peroxidation markers did not significantly decrease, in spite of a trend, after the first and/or the second dose of DHA but plasma and platelet vitamin E amounts increased significantly after DHA supplementation. Our findings reinforce the antioxidant potential of moderate DHA intake in subjects displaying increased oxidative stress.
authors.affiliation: Univ-Lyon, CarMeN laboratory, Inserm U1060, INRA U1397, Université Claude Bernard Lyon 1, INSA-Lyon, IMBL, 69621 Villeurbanne, France. Electronic address: evelyne.vericel@insa-lyon.fr.
authors.forename: Evelyne
authors.initials: E
authors.lastname: Véricel
authors.affiliation: Centre de Référence pédiatrique Mucoviscidose de Lyon, Hôpital Femme Mère Enfant, F-69500 Bron, France.
authors.forename: Stéphane
authors.initials: S
authors.lastname: Mazur
authors.affiliation: Univ-Lyon, CarMeN laboratory, Inserm U1060, INRA U1397, Université Claude Bernard Lyon 1, INSA-Lyon, IMBL, 69621 Villeurbanne, France.
authors.forename: Romain
authors.initials: R
authors.lastname: Colas
authors.affiliation: Centre de Référence pédiatrique Mucoviscidose de Lyon, Hôpital Femme Mère Enfant, F-69500 Bron, France.
authors.forename: Véronique
authors.initials: V
authors.lastname: Delaup
authors.affiliation: Univ-Lyon, CarMeN laboratory, Inserm U1060, INRA U1397, Université Claude Bernard Lyon 1, INSA-Lyon, IMBL, 69621 Villeurbanne, France.
authors.forename: Catherine
authors.initials: C
authors.lastname: Calzada
authors.affiliation: Centre de Référence pédiatrique Mucoviscidose de Lyon, Hôpital Femme Mère Enfant, F-69500 Bron, France.
authors.forename: Philippe
authors.initials: P
authors.lastname: Reix
authors.affiliation: Centre de Référence adulte Mucoviscidose de Lyon, Centre Hospitalier Lyon-Sud, F-69310 Pierre-Bénite, France.
authors.forename: Isabelle
authors.initials: I
authors.lastname: Durieu
authors.affiliation: Univ-Lyon, CarMeN laboratory, Inserm U1060, INRA U1397, Université Claude Bernard Lyon 1, INSA-Lyon, IMBL, 69621 Villeurbanne, France.
authors.forename: Michel
authors.initials: M
authors.lastname: Lagarde
authors.affiliation: Centre de Référence pédiatrique Mucoviscidose de Lyon, Hôpital Femme Mère Enfant, F-69500 Bron, France.
authors.forename: Gabriel
authors.initials: G
authors.lastname: Bellon
mesh_terms: D000293:Adolescent; D000328:Adult; D001792:Blood Platelets / Q000378:metabolism*; D002648:Child; D018592:Cross-Over Studies; D003550:Cystic Fibrosis / Q000097:blood*; D004281:Docosahexaenoic Acids / Q000008:administration & dosage* / Q000494:pharmacology; D004311:Double-Blind Method; D004334:Drug Administration Schedule; D006801:Humans; D015227:Lipid Peroxidation; D008297:Male; D018384:Oxidative Stress / Q000235:genetics; D014810:Vitamin E / Q000097:blood*; D055815:Young Adult
chemical_list: D014810:Vitamin E; D004281:Docosahexaenoic Acids
keywords: Antioxidant status; Docosahexaenoic acid; Oxidative stress; Plasma; Platelet lipids