title: Targeting Trichothecene Biosynthetic Genes.
abstract: Biosynthesis of trichothecenes requires the involvement of at least 15 genes, most of which have been targeted for PCR. Qualitative PCRs are used to assign chemotypes to individual isolates, e.g., the capacity to produce type A and/or type B trichothecenes. Many regions in the core cluster (consisting of 12 genes) including intergenic regions have been used as targets for PCR, but the most robust assays are targeted to the tri3 and tri12 genes. Quantitative PCRs, that work across trichothecene-producing members of the Fusarium head blight complex, are described along with procedures to quantify the amount of fungal biomass in wheat samples. These assays are directed to the chemotype(s) present in field samples and quantify the total fungal biomass of trichothecene-producing fungi, irrespective of their genetic identity.
authors.affiliation: College of Plant Protection, Shenyang Agricultural University, No. 120 Dongling Road, Shenyang, Liaoning, People's Republic of China.
authors.forename: Songhong
authors.initials: S
authors.lastname: Wei
authors.affiliation: Biointeractions and Plant Health, Wageningen UR, Droevendaalsesteeg 1, Wageningen, The Netherlands.
authors.forename: Theo
authors.initials: T
authors.lastname: van der Lee
authors.affiliation: Biointeractions and Plant Health, Wageningen UR, Droevendaalsesteeg 1, Wageningen, The Netherlands.
authors.forename: Els
authors.initials: E
authors.lastname: Verstappen
authors.affiliation: Biointeractions and Plant Health, Wageningen UR, Droevendaalsesteeg 1, Wageningen, The Netherlands.
authors.forename: Marga
authors.initials: M
authors.lastname: van Gent
authors.affiliation: Biointeractions and Plant Health, Wageningen UR, Droevendaalsesteeg 1, Wageningen, The Netherlands. cees.waalwijk@wur.nl.
authors.forename: Cees
authors.initials: C
authors.lastname: Waalwijk
mesh_terms: D005670:Fusarium / Q000235:genetics / Q000378:metabolism; D005800:Genes, Fungal; D059014:High-Throughput Nucleotide Sequencing; D005810:Multigene Family; D020641:Polymorphism, Single Nucleotide; D060888:Real-Time Polymerase Chain Reaction; D014255:Trichothecenes / Q000096:biosynthesis*; D014908:Triticum / Q000382:microbiology
chemical_list: D014255:Trichothecenes
keywords: PCR; Quantitative PCR; Trichothecenes