title: In Silico Characterization of miRNA and Long Non-Coding RNA Interplay in Multiple Myeloma.
abstract: The identification of deregulated microRNAs (miRNAs) and long non-coding RNAs (lncRNAs) in multiple myeloma (MM) has progressively added a further level of complexity to MM biology. In addition, the cross-regulation between lncRNAs and miRNAs has begun to emerge, and theoretical and experimental studies have demonstrated the competing endogenous RNA (ceRNA) activity of lncRNAs as natural miRNA decoys in pathophysiological conditions, including cancer. Currently, information concerning lncRNA and miRNA interplay in MM is virtually absent. Herein, we investigated in silico the lncRNA and miRNA relationship in a representative datasets encompassing 95 MM and 30 plasma cell leukemia patients at diagnosis and in four normal controls, whose expression profiles were generated by a custom annotation pipeline to detect specific lncRNAs. We applied target prediction analysis based on miRanda and RNA22 algorithms to 235 lncRNAs and 459 miRNAs selected with a potential pivotal role in the pathology of MM. Among pairs that showed a significant correlation between lncRNA and miRNA expression levels, we identified 11 lncRNA-miRNA relationships suggestive of a novel ceRNA network with relevance in MM.
authors.affiliation: Department of Oncology and Hemato-Oncology, University of Milano, 20122 Milan, Italy. domenica.ronchetti@unimi.it.|Hematology Unit, Fondazione IRCCS Ca' Granda, Ospedale Maggiore Policlinico, 20122 Milan, Italy. domenica.ronchetti@unimi.it.
authors.forename: Domenica
authors.initials: D
authors.lastname: Ronchetti
authors.affiliation: Department of Oncology and Hemato-Oncology, University of Milano, 20122 Milan, Italy. martina.manzoni@unimi.it.|Hematology Unit, Fondazione IRCCS Ca' Granda, Ospedale Maggiore Policlinico, 20122 Milan, Italy. martina.manzoni@unimi.it.
authors.forename: Martina
authors.initials: M
authors.lastname: Manzoni
authors.affiliation: Laboratory of Pre-Clinical and Translational Research, IRCCS-CROB, Referral Cancer Center of Basilicata, 85028 Rionero in Vulture (PZ), Italy. katia.todoerti@gmail.com.
authors.forename: Katia
authors.initials: K
authors.lastname: Todoerti
authors.affiliation: Department of Oncology and Hemato-Oncology, University of Milano, 20122 Milan, Italy. antonino.neri@unimi.it.|Hematology Unit, Fondazione IRCCS Ca' Granda, Ospedale Maggiore Policlinico, 20122 Milan, Italy. antonino.neri@unimi.it.
authors.forename: Antonino
authors.initials: A
authors.lastname: Neri
authors.affiliation: Department of Oncology and Hemato-Oncology, University of Milano, 20122 Milan, Italy. luca.agnelli@gmail.com.|Hematology Unit, Fondazione IRCCS Ca' Granda, Ospedale Maggiore Policlinico, 20122 Milan, Italy. luca.agnelli@gmail.com.
authors.forename: Luca
authors.initials: L
authors.lastname: Agnelli
keywords: lncRNA; miRNA; multiple myeloma