title: First evaluation of an influenza viral vector based Brucella abortus vaccine in sheep and goats: Assessment of safety, immunogenicity and protective efficacy against Brucella melitensis infection.
abstract: Previously we developed and evaluated a candidate influenza viral vector based Brucella abortus vaccine (Flu-BA) administered with a potent adjuvant Montanide Gel01 in cattle, which was found safe and highly effective. This study was aimed to establish a proof-of-concept of the efficacy of Flu-BA vaccine formulation in sheep and goats. We vaccinated sheep and goats with Flu-BA vaccine and as a positive control vaccinated a group of animals with a commercial B. melitensis Rev.1 vaccine. Clinically, both Flu-BA and Rev.1 vaccines were found safe. Serological analysis showed the animals received Flu-BA vaccine did not induce antibody response against Brucella Omp16 and L7/L12 proteins during the period of our study (56days post-initial vaccination, PIV). But observed significant antigen-specific T cell response indicated by increased lymphocyte stimulation index and enhanced secretion of IFN-γ at day 56 PIV in Flu-BA group. The Flu-BA vaccinated animals completely protected 57.1% of sheep and 42.9% of goats against B. melitensis 16M challenge. The severity of brucellosis in terms of infection index and colonization of Brucella in tissues was significantly lower in the Flu-BA group compared to negative control animals group. Nevertheless, positive control commercial Rev.1 vaccine provided strong antigen-specific T cell immunity and protection against B. melitensis 16M infection. We conclude that the Flu-BA vaccine induces a significant antigen-specific T-cell response and provides complete protection in approximately 50% of sheep and goats against B. melitensis 16M infection. Further investigations are needed to improve the efficacy of Flu-BA and explore its practical application in small ruminants.
authors.affiliation: The Research Institute for Biological Safety Problems, Zhambulskaya oblast, Kordaiskiy rayon, 080409, Gvardeiskiy, Kazakhstan. Electronic address: tabynov_81@mail.ru.
authors.forename: Kaissar
authors.initials: K
authors.lastname: Tabynov
authors.affiliation: The Research Institute for Biological Safety Problems, Zhambulskaya oblast, Kordaiskiy rayon, 080409, Gvardeiskiy, Kazakhstan.
authors.forename: Bolat
authors.initials: B
authors.lastname: Yespembetov
authors.affiliation: Faculty of Veterinary Science, Kazakh National Agrarian University (KazNAU), Almaty 050010, Kazakhstan.
authors.forename: Nurali
authors.initials: N
authors.lastname: Matikhan
authors.affiliation: The Research Institute for Biological Safety Problems, Zhambulskaya oblast, Kordaiskiy rayon, 080409, Gvardeiskiy, Kazakhstan.
authors.forename: Sholpan
authors.initials: S
authors.lastname: Ryskeldinova
authors.affiliation: The Research Institute for Biological Safety Problems, Zhambulskaya oblast, Kordaiskiy rayon, 080409, Gvardeiskiy, Kazakhstan.
authors.forename: Nadezhda
authors.initials: N
authors.lastname: Zinina
authors.affiliation: The Research Institute for Biological Safety Problems, Zhambulskaya oblast, Kordaiskiy rayon, 080409, Gvardeiskiy, Kazakhstan.
authors.forename: Zhailaubay
authors.initials: Z
authors.lastname: Kydyrbayev
authors.affiliation: The Research Institute for Biological Safety Problems, Zhambulskaya oblast, Kordaiskiy rayon, 080409, Gvardeiskiy, Kazakhstan.
authors.forename: Nurika
authors.initials: N
authors.lastname: Assanzhanova
authors.affiliation: Faculty of Veterinary Science, Kazakh National Agrarian University (KazNAU), Almaty 050010, Kazakhstan.
authors.forename: Kairat
authors.initials: K
authors.lastname: Tabynov
authors.affiliation: Food Animal Health Research Program, Ohio Agricultural Research and Development Center, Department of Veterinary Preventive Medicine, The Ohio State University (OSU), Wooster, OH 44691, USA.
authors.forename: Gourapura J
authors.initials: GJ
authors.lastname: Renukaradhya
authors.affiliation: Faculty of Veterinary Science, Kazakh National Agrarian University (KazNAU), Almaty 050010, Kazakhstan.
authors.forename: Gulnara
authors.initials: G
authors.lastname: Mukhitdinova
authors.affiliation: The Research Institute for Biological Safety Problems, Zhambulskaya oblast, Kordaiskiy rayon, 080409, Gvardeiskiy, Kazakhstan.
authors.forename: Abylai
authors.initials: A
authors.lastname: Sansyzbay
mesh_terms: D000818:Animals; D002004:Brucella Vaccine / Q000276:immunology*; D002003:Brucella abortus; D017347:Brucella melitensis; D002006:Brucellosis / Q000382:microbiology / Q000517:prevention & control / Q000662:veterinary*; D004337:Drug Carriers; D015511:Goat Diseases / Q000517:prevention & control*; D006041:Goats; D009975:Orthomyxoviridae / Q000235:genetics; D012756:Sheep; D012757:Sheep Diseases / Q000517:prevention & control*; D014614:Vaccines, Synthetic / Q000276:immunology
chemical_list: D002004:Brucella Vaccine; D004337:Drug Carriers; D014614:Vaccines, Synthetic
keywords: Antibody and T-cell-mediated immune response; Brucella melitensis; Influenza viral vector; Protective efficacy; Safety; Sheep and goats; Vaccine