title: Giant Oyster Mushroom Pleurotus giganteus (Agaricomycetes) Enhances Adipocyte Differentiation and Glucose Uptake via Activation of PPARγ and Glucose Transporters 1 and 4 in 3T3-L1 Cells.
abstract: The edible mushroom Pleurotus giganteus was tested for its effect on adipocyte differentiation and glucose uptake activity in 3T3-L1 cells. The basidiocarps of P. giganteus were soaked in methanol to obtain a crude methanol extract and then fractionated to obtain an ethyl acetate extract. In this study, cell proliferation was measured using an MTT assay, lipid accumulation using an Oil Red O assay, and glucose uptake using a fluorescence glucose uptake assay. Gene expression was measured via real-time polymerase chain reaction analysis with TaqMan primer. Ethyl acetate extract significantly enhanced adipogenic differentiation and glucose uptake in 3T3-L1 adipocytes via the expression of sterol regulatory element-binding protein, peroxisome proliferator-activated receptor γ, and phos-phatidylinositol 3-kinase/Akt. Glucose uptake was facilitated by the highly expressed glucose transporters Glut1 and Glut4. Taken together, these results suggest that P. giganteus ethyl acetate extract has an insulin-sensitizing effect on adipocytes and has potential as an adjuvant for the management of type 2 diabetes.
authors.affiliation: Mushroom Research Centre (MRC), Institute of Biological Sciences, Faculty of Science, University of Malaya, Kuala Lumpur, Malaysia; Institute of Biological Sciences, Faculty of Science, University of Malaya, Kuala Lumpur, Malaysia.
authors.forename: Puvaneswari
authors.initials: P
authors.lastname: Paravamsivam
authors.affiliation: Mushroom Research Centre (MRC), Institute of Biological Sciences, Faculty of Science, University of Malaya, Kuala Lumpur, Malaysia; Department of Biomedical Science, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia.
authors.forename: Chua Kek
authors.initials: CK
authors.lastname: Heng
authors.affiliation: Mushroom Research Centre, Fungal Biotechnology Lab.; Institute of Biological Sciences, Faculty of Science, University of Malaya, Kuala Lumpur, Malaysia.
authors.forename: Sri Nurestri Abdul
authors.initials: SN
authors.lastname: Malek
authors.affiliation: Mushroom Research Centre, Institute of Biological Sciences, Faculty of Science, University of Malaya, Kuala Lumpur, Malaysia; Institute of Biological Sciences, Faculty of Science, University of Malaya, Kuala Lumpur, Malaysia.
authors.forename: Vikineswary
authors.initials: V
authors.lastname: Sabaratnam
authors.affiliation: Department of Biomedical Science, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia.
authors.forename: Ravishankar Ram
authors.initials: RR
authors.lastname: M
authors.affiliation: Mushroom Research Centre (MRC), Institute of Biological Sciences, Faculty of Science, University of Malaya, Kuala Lumpur, Malaysia; Department of Biomedical Science, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia.
authors.forename: Umah Rani
authors.initials: UR
authors.lastname: Kuppusamy
mesh_terms: D041721:3T3-L1 Cells; D000085:Acetates / Q000737:chemistry; D017667:Adipocytes / Q000187:drug effects*; D000818:Animals; D002454:Cell Differentiation; D005947:Glucose / Q000378:metabolism*; D051246:Glucose Transport Proteins, Facilitative / Q000378:metabolism*; D000432:Methanol / Q000737:chemistry; D051379:Mice; D047495:PPAR gamma / Q000378:metabolism*; D020076:Pleurotus / Q000737:chemistry*
chemical_list: D000085:Acetates; D051246:Glucose Transport Proteins, Facilitative; D047495:PPAR gamma; C007650:ethyl acetate; D005947:Glucose; D000432:Methanol