title: Finding Potential Therapeutic Targets againstShigella flexnerithrough Proteome Exploration.
abstract: Background:Shigella flexneriis a gram negative bacteria that causes the infectious disease "shigellosis."S. flexneriis responsible for developing diarrhea, fever, and stomach cramps in human. Antibiotics are mostly given to patients infected with shigella. Resistance to antibiotics can hinder its treatment significantly. Upon identification of essential therapeutic targets, vaccine and drug could be effective therapy for the treatment of shigellosis.Methods:The study was designed for the identification and qualitative characterization for potential drug targets fromS. flexneriby using the subtractive proteome analysis. A set of computational tools were used to identify essential proteins those are required for the survival ofS. flexneri. Total proteome (13,503 proteins) ofS. flexneriwas retrieved from NCBI and further analyzed by subtractive channel analysis. After identification of the metabolic proteins we have also performed its qualitative characterization to pave the way for the identification of promising drug targets.Results:Subtractive analysis revealed that a list of 53 targets ofS. flexneriwere human non-homologous essential metabolic proteins that might be used for potential drug targets. We have also found that 11 drug targets are involved in unique pathway. Most of these proteins are cytoplasmic, can be used as broad spectrum drug targets, can interact with other proteins and show the druggable properties. The functionality and drug binding site analysis suggest a promising effective way to design the new drugs againstS. flexneri.Conclusion:Among the 53 therapeutic targets identified through this study, 13 were found highly potential as drug targets based on their physicochemical properties whilst only one was found as vaccine target againstS. flexneri. The outcome might also be used as module as well as circuit design in systems biology.
authors.affiliation: Department of Biotechnology and Genetic Engineering, Life Science Faculty, Mawlana Bhashani Science and Technology University Tangail, Bangladesh.
authors.forename: Mohammad Uzzal
authors.initials: MU
authors.lastname: Hossain
authors.affiliation: Department of Science and Humanities, Military Institute of Science and Technology, Mirpur Cantonment Dhaka, Bangladesh.
authors.forename: Md Arif
authors.initials: MA
authors.lastname: Khan
authors.affiliation: Microbial Biotechnology Division, National Institute of Biotechnology Savar, Bangladesh.
authors.forename: Abu
authors.initials: A
authors.lastname: Hashem
authors.affiliation: Department of Biotechnology and Genetic Engineering, Life Science Faculty, Mawlana Bhashani Science and Technology University Tangail, Bangladesh.
authors.forename: Md Monirul
authors.initials: MM
authors.lastname: Islam
authors.affiliation: Department of Science and Humanities, Military Institute of Science and Technology, Mirpur Cantonment Dhaka, Bangladesh.
authors.forename: Mohammad Neaz
authors.initials: MN
authors.lastname: Morshed
authors.affiliation: Department of Biochemistry and Microbiology, North South University Bashundhara, Dhaka, Bangladesh.
authors.forename: Chaman Ara
authors.initials: CA
authors.lastname: Keya
authors.affiliation: Molecular Biotechnology Division, National Institute of Biotechnology Savar, Bangladesh.
authors.forename: Md
authors.initials: M
authors.lastname: Salimullah
keywords: S. flexneri; drug target; metabolic proteins; proteome; therapeutics