title: Establishment and biological characterization of a dermal mesenchymal stem cells line from bovine.
abstract: The DMSCs (dermal mesenchymal stem cells) are multipotent stem cells, which can differentiate in vitro into many cell types. Much work has been done on DMSCs from humans, mice, rabbits and other mammals, but the related literature has not been published about these cells in cattle. In this study, we isolated and established the DMSC lines from cattle, thereby initiating further research on these cells, such as growth kinetics, detection of special surface antigen and RT-PCR (reverse transcription-PCR) assays to identify the biological characterization of the cell line. Furthermore, the DMSCs are induced to differentiate into adipocytes, osteoblasts and neural cells in vitro Our results suggest that DMSCs isolated from cattle possess similar biological characteristics with those from other species. Their multi-lineage differentiation capabilities herald a probable application model in tissue engineering and induced pluripotent stem cells.
authors.affiliation: Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, People's Republic of China.|Institute of Basic Medicine, Jiamusi University, Jiamusi 154007, People's Republic of China.
authors.forename: Tingting
authors.initials: T
authors.lastname: Sun
authors.affiliation: Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, People's Republic of China.
authors.forename: Chao
authors.initials: C
authors.lastname: Yu
authors.affiliation: Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, People's Republic of China.
authors.forename: Yuhua
authors.initials: Y
authors.lastname: Gao
authors.affiliation: Harbin Institute Of Physical Education, Harbin 150000, People's Republic of China.
authors.forename: Chenqiong
authors.initials: C
authors.lastname: Zhao
authors.affiliation: College of Veterinary Medicine, Shaanxi Centre of Stem Cells Engineering & Technology, Key Laboratory for Animal Biotechnology of Agriculture Ministry of China, Northwest A & F University, Yangling, Shanxi 712100, People's Republic of China.
authors.forename: Jinlian
authors.initials: J
authors.lastname: Hua
authors.affiliation: Institute of Basic Medicine, Jiamusi University, Jiamusi 154007, People's Republic of China.
authors.forename: Lianshun
authors.initials: L
authors.lastname: Cai
authors.affiliation: Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, People's Republic of China yuehuima@hotmail.com weijunguan301@gmail.com.
authors.forename: Weijun
authors.initials: W
authors.lastname: Guan
authors.affiliation: Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, People's Republic of China yuehuima@hotmail.com weijunguan301@gmail.com.
authors.forename: Yuehui
authors.initials: Y
authors.lastname: Ma
mesh_terms: D017667:Adipocytes / Q000166:cytology / Q000378:metabolism; D000818:Animals; D002417:Cattle; D002454:Cell Differentiation; D002460:Cell Line; D020405:Dermis / Q000166:cytology* / Q000378:metabolism*; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism*; D010006:Osteoblasts / Q000166:cytology / Q000378:metabolism; D023822:Tissue Engineering
keywords: bovine; cell culture; dermal mesenchymal stem cell (DMSC); multi-lineage differentiation