title: Evaluation of cardioprotective effect of aqueous extract ofAllium cepaLinn. bulb on isoprenaline-induced myocardial injury in Wistar albino rats.
abstract: To investigate the cardioprotective potential of the aqueous extract ofAllium cepaLinn. bulb in isoprenaline-induced myocardial injury in Wistar albino rats.In vitrototal phenolic, total flavonoid content and 2, 2'-diphenyl-1-picrylhydrazyl hydrate radical scavenging activity was measured. Isoprenaline-induced myocardial injury model was used to evaluatein vivoeffect of aqueous extract ofA. cepain Wistar albino rats. Seventy two rats were randomly divided in 6 groups. Rats were treated withA. cepa400 mg/kg and 800 mg/kg doses for 30 days and myocardial injury was produced by subcutaneous injection of isoprenaline (ISO) 85 mg/kg on day 28 and 29. Carvedilol 1 mg/kg for 30 days served as active control. Electrocardiogram parameters, cardiac injury markers, oxidative stress markers and histopathological changes were evaluated in each group and compared using appropriate statistical tests.In vitroevaluation of aqueous extract ofA. cepashowed significant antioxidant property. ISO produced significant myocardial injury as compared to normal control group (P< 0.05). Administration ofA. cepain the dose of 400 mg/kg significantly recovered the altered parameters (Troponin-I, Creatine kinase-MB, glutamate-pyruvate transaminase, HR, R-R interval, and oxidative stress markers) compared to disease control group (P< 0.05) whileA. cepain the dose 800 mg/kg recovered the altered parameters (HR, heart weight/body weight ratio, and superoxide dismutase level) compared to disease control group. Histopathological parameters did not recover in the doses of 400 and 800 mg/kg (P> 0.05). The aqueous extract ofA. cepa400 mg/kg was found to be cardioprotective against myocardial injury whileA. cepa800 mg/kg did not show significant cardioprotective activity. So, we presume thatA. cepamight be effective within certain dose range only.
authors.affiliation: Department of Pharmacology, Government Medical College, Bhavnagar-364001, Gujarat, India.
authors.forename: Geeta B
authors.initials: GB
authors.lastname: Kharadi
authors.affiliation: Department of Pharmacology, Government Medical College, Bhavnagar-364001, Gujarat, India.
authors.forename: Kaksha J
authors.initials: KJ
authors.lastname: Patel
authors.affiliation: Department of Pharmacology, Government Medical College, Bhavnagar-364001, Gujarat, India.
authors.forename: Bhargav M
authors.initials: BM
authors.lastname: Purohit
authors.affiliation: Department of Pathology, Government Medical College, Bhavnagar-364001, Gujarat, India.
authors.forename: Seema N
authors.initials: SN
authors.lastname: Baxi
authors.affiliation: Department of Pharmacology, Government Medical College, Bhavnagar-364001, Gujarat, India.
authors.forename: C B
authors.initials: CB
authors.lastname: Tripathi
keywords: Allium cepa; Cardioprotective; Carvedilol; Isoprenaline; Myocardial infarction