title: Fast spectral surface plasmon resonance imaging sensor for real-time high-throughput detection of biomolecular interactions.
abstract: A fast surface plasmon resonance (SPR) imaging biosensor system based on wavelength interrogation using a liquid crystal tunable filter (LCTF) is presented. The system combines the merits of wide-dynamic detection range offered by the spectral approach and multiplexed high-throughput data collection with a two-dimensional (2-D) biosensor array. The key feature of the reported scheme is a feedback loop that drives the LCTF to achieve fast tracking of the SPR dip movement caused by the binding of target molecules to the sensor surface. Experimental results show that the system is capable of completing an SPR dip measurement within 4 s. Based on using a spectral window of about 100 nm, the experimental dynamic detection range and refractive index resolution are 4.63×10?2??RIU and 5.87×10?6??RIU, respectively. As also demonstrated herein using 2-D microsensor arrays, among the spectral SPR sensors, the reported system is most suitable for multiplexed label-free biosensing applications.
authors.affiliation: Shenzhen University, College of Optoelectronic Engineering, Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, Shenzhen Key Laboratory of Sensor Technology, Shenzhen 518060, China.
authors.forename: Kaiqiang
authors.initials: K
authors.lastname: Chen
authors.affiliation: Shenzhen University, College of Optoelectronic Engineering, Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, Shenzhen Key Laboratory of Sensor Technology, Shenzhen 518060, China.
authors.forename: Youjun
authors.initials: Y
authors.lastname: Zeng
authors.affiliation: Shenzhen University, College of Optoelectronic Engineering, Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, Shenzhen Key Laboratory of Sensor Technology, Shenzhen 518060, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Wang
authors.affiliation: Shenzhen Entry-Exit Inspection and Quarantine Bureau, Shenzhen 518033, China.
authors.forename: Dayong
authors.initials: D
authors.lastname: Gu
authors.affiliation: Shenzhen Entry-Exit Inspection and Quarantine Bureau, Shenzhen 518033, China.
authors.forename: Jianan
authors.initials: J
authors.lastname: He
authors.affiliation: Chinese University of Hong Kong, Department of Electronic Engineering, Shatin, NT, Hong Kong.
authors.forename: Shu-Yuen
authors.initials: SY
authors.lastname: Wu
authors.affiliation: Chinese University of Hong Kong, Department of Electronic Engineering, Shatin, NT, Hong Kong.
authors.forename: Ho-Pui
authors.initials: HP
authors.lastname: Ho
authors.affiliation: Shenzhen University, College of Optoelectronic Engineering, Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, Shenzhen Key Laboratory of Sensor Technology, Shenzhen 518060, China.
authors.forename: Xuejin
authors.initials: X
authors.lastname: Li
authors.affiliation: Shenzhen University, College of Optoelectronic Engineering, Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, Shenzhen Key Laboratory of Sensor Technology, Shenzhen 518060, China.
authors.forename: Junle
authors.initials: J
authors.lastname: Qu
authors.affiliation: Clemson University, Department of Bioengineering and COMSET, Clemson, South Carolina 29634, United States.
authors.forename: Bruce Zhi
authors.initials: BZ
authors.lastname: Gao
authors.affiliation: Shenzhen University, College of Optoelectronic Engineering, Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, Shenzhen Key Laboratory of Sensor Technology, Shenzhen 518060, China.
authors.forename: Yonghong
authors.initials: Y
authors.lastname: Shao
mesh_terms: D000818:Animals; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D004867:Equipment Design; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D007074:Immunoglobulin G / Q000032:analysis; D050866:Liquid Crystals; D046228:Microarray Analysis; D059629:Signal-To-Noise Ratio; D020349:Surface Plasmon Resonance / Q000295:instrumentation / Q000379:methods*
chemical_list: D007074:Immunoglobulin G