title: Digital gene expression profiling analysis of DNA repair pathways in colon cancer stem population of HT29 cells.
abstract: Cancer stem cells (CSCs) contribute to the relapse and development of new neoplasm lesions. While most available clinical approaches, such as chemical and radiation therapies, will kill the majority of cancer cells, they do not kill them all. Some resisting cells, like CSCs, are able to survive due to their excellent self-maintaining capabilities, even in challenging environments. In the present study, we investigated the mRNA level of DNA repair genes of colon CSCs from the HT29 cell line in response to single-strand damage and double-strand breaks, as well as the evident upregulation of key genes in base excision repair, mismatch repair, non-homologous end-joining, and homologous recombination pathways in these cells. Digital gene expression analysis identified upregulated genes in CD44+HT29 cells that may play important roles in DNA repair. Our results reveal that colon CSCs bear efficient DNA repair abilities, which might explain the survival of colon CSCs after repeated chemical and radiation therapy.
authors.affiliation: Laboratory of Biochemistry and Molecular Biology, School of Life Sciences, Yunnan University, Kunming 650091, China.
authors.forename: Wenxue
authors.initials: W
authors.lastname: Wang
authors.affiliation: Laboratory of Biochemistry and Molecular Biology, School of Life Sciences, Yunnan University, Kunming 650091, China.
authors.forename: Guoxiu
authors.initials: G
authors.lastname: Zhang
authors.affiliation: Laboratory of Biochemistry and Molecular Biology, School of Life Sciences, Yunnan University, Kunming 650091, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Yang
authors.affiliation: Laboratory of Biochemistry and Molecular Biology, School of Life Sciences, Yunnan University, Kunming 650091, China.
authors.forename: Huan
authors.initials: H
authors.lastname: Gu
authors.affiliation: Laboratory of Biochemistry and Molecular Biology, School of Life Sciences, Yunnan University, Kunming 650091, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Ding
authors.affiliation: Laboratory of Biochemistry and Molecular Biology, School of Life Sciences, Yunnan University, Kunming 650091, China.
authors.forename: Haijing
authors.initials: H
authors.lastname: Yu
authors.affiliation: Laboratory of Biochemistry and Molecular Biology, School of Life Sciences, Yunnan University, Kunming 650091, China.
authors.forename: Min
authors.initials: M
authors.lastname: Yu
authors.affiliation: Laboratory of Biochemistry and Molecular Biology, School of Life Sciences, Yunnan University, Kunming 650091, China.
authors.forename: Qinghua
authors.initials: Q
authors.lastname: Cui
authors.affiliation: State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Yunnan University, Kunming 650091, China rich@ynu.edu.cn mzhli@ynu.edu.cn.
authors.forename: Xinglai
authors.initials: X
authors.lastname: Ji
authors.affiliation: Laboratory of Biochemistry and Molecular Biology, School of Life Sciences, Yunnan University, Kunming 650091, China rich@ynu.edu.cn mzhli@ynu.edu.cn.
authors.forename: Meizhang
authors.initials: M
authors.lastname: Li
mesh_terms: D003110:Colonic Neoplasms / Q000235:genetics* / Q000473:pathology; D004260:DNA Repair / Q000235:genetics*; D020869:Gene Expression Profiling; D019073:HT29 Cells; D006801:Humans; D014411:Neoplastic Stem Cells / Q000378:metabolism*
keywords: DNA repair; cancer stem cells; colon carcinoma; digital gene expression