title: Fabrication and characterization of drug-loaded nano-hydroxyapatite/polyamide 66 scaffolds modified with carbon nanotubes and silk fibroin.
abstract: Nano-hydroxyapatite/polyamide 66 (nHA/PA66) porous scaffolds were fabricated by a phase inversion method. Carbon nanotubes (CNTs) and silk fibroin (SF) were used to modify the surface of the nHA/PA66 scaffolds by freeze-drying and cross-linking. Dexamethasone was absorbed to the CNTs to promote the osteogenic differentiation of bone mesenchymal stem cells (BMSCs). The cell viability of BMSCs was investigated by changing the concentration of the CNT dispersion, and the most biocompatible scaffold was selected. In addition, the morphology and mechanical property of the scaffolds were investigated. The results showed that the nHA/PA66 scaffolds modified with CNTs and SF met the requirements of bone tissue engineering scaffolds. The dexamethasone-loaded CNT/SF-nHA/PA66 composite scaffold promoted the osteogenic differentiation of BMSCs, and the drug-loaded scaffolds are expected to function as effective bone tissue engineering scaffolds.
authors.affiliation: Institute of Pharmaceutics, College of Pharmaceutical Sciences, Zhejiang University.
authors.forename: Meng-Zhu
authors.initials: MZ
authors.lastname: Yao
authors.affiliation: Institute of Pharmaceutics, College of Pharmaceutical Sciences, Zhejiang University.
authors.forename: Ming-Yi
authors.initials: MY
authors.lastname: Huang-Fu
authors.affiliation: Institute of Pharmaceutics, College of Pharmaceutical Sciences, Zhejiang University.
authors.forename: Hui-Na
authors.initials: HN
authors.lastname: Liu
authors.affiliation: Institute of Pharmaceutics, College of Pharmaceutical Sciences, Zhejiang University.
authors.forename: Xia-Rong
authors.initials: XR
authors.lastname: Wang
authors.affiliation: Hangzhou SoliPharma Co., Ltd, Hangzhou, Zhejiang, People's Republic of China.
authors.forename: Xiaoxia
authors.initials: X
authors.lastname: Sheng
authors.affiliation: Institute of Pharmaceutics, College of Pharmaceutical Sciences, Zhejiang University.
authors.forename: Jian-Qing
authors.initials: JQ
authors.lastname: Gao
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology; D001672:Biocompatible Materials / Q000737:chemistry / Q000494:pharmacology*; D001842:Bone and Bones / Q000166:cytology / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D003907:Dexamethasone / Q000494:pharmacology; D017886:Durapatite / Q000737:chemistry / Q000494:pharmacology*; D005349:Fibroins / Q000737:chemistry*; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000187:drug effects; D037742:Nanotubes, Carbon / Q000737:chemistry*; D009757:Nylons / Q000737:chemistry / Q000494:pharmacology*; D010012:Osteogenesis / Q000187:drug effects; D016062:Porosity; D051381:Rats; D017207:Rats, Sprague-Dawley; D047011:Silk / Q000737:chemistry*; D023822:Tissue Engineering / Q000379:methods; D054457:Tissue Scaffolds
chemical_list: D000893:Anti-Inflammatory Agents; D001672:Biocompatible Materials; D037742:Nanotubes, Carbon; D009757:Nylons; D047011:Silk; D003907:Dexamethasone; D005349:Fibroins; D017886:Durapatite
keywords: BMSCs; carbon nanotubes; dexamethasone; porous scaffold; silk fibroin; surface modification; tissue engineering