title: Multi-omics approach to study global changes in a triclosan-resistant mutant strain of Acinetobacter baumannii ATCC 17978.
abstract: Acinetobacter baumannii AB042, a triclosan-resistant mutant strain, was examined for modulated gene expression using whole-genome sequencing, transcriptomics and proteomics in order to understand the mechanism of triclosan resistance as well as its impact on A. baumannii. Data revealed modulated expression of the fatty acid metabolism pathway, co-factors known to play a role in the synthesis of fatty acids, as well as several transcriptional regulators. The membrane composition of the mutant revealed a decrease in C18with a corresponding increase in C16fatty acids compared with the parent strain A. baumannii ATCC 17978. These data indicate that A. baumannii responds to triclosan by altering the expression of genes involved in fatty acid metabolism, antibiotic resistance and amino acid metabolism.
authors.affiliation: Department of Microbiology, University of Manitoba, Winnipeg, MB R3T 2N2, Canada.
authors.forename: Dinesh M
authors.initials: DM
authors.lastname: Fernando
authors.affiliation: National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, MB, Canada.
authors.forename: Patrick
authors.initials: P
authors.lastname: Chong
authors.affiliation: Department of Microbiology, University of Manitoba, Winnipeg, MB R3T 2N2, Canada.
authors.forename: Manu
authors.initials: M
authors.lastname: Singh
authors.affiliation: Department of Internal Medicine, University of Manitoba, Winnipeg, MB R3T 2N2, Canada.
authors.forename: Victor
authors.initials: V
authors.lastname: Spicer
authors.affiliation: Department of Microbiology, University of Manitoba, Winnipeg, MB R3T 2N2, Canada.
authors.forename: Mark
authors.initials: M
authors.lastname: Unger
authors.affiliation: Department of Microbiology, University of Manitoba, Winnipeg, MB R3T 2N2, Canada.
authors.forename: Peter C
authors.initials: PC
authors.lastname: Loewen
authors.affiliation: National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, MB, Canada.
authors.forename: Garrett
authors.initials: G
authors.lastname: Westmacott
authors.affiliation: Department of Microbiology, University of Manitoba, Winnipeg, MB R3T 2N2, Canada; Department of Medical Microbiology, University of Manitoba, Winnipeg, MB R3T 2N2, Canada. Electronic address: Ayush.Kumar@umanitoba.ca.
authors.forename: Ayush
authors.initials: A
authors.lastname: Kumar
mesh_terms: D040981:Acinetobacter baumannii / Q000187:drug effects*; D000891:Anti-Infective Agents, Local / Q000494:pharmacology*; D024881:Drug Resistance, Bacterial; D020869:Gene Expression Profiling; D016680:Genome, Bacterial; D050356:Lipid Metabolism; D053858:Metabolic Networks and Pathways / Q000235:genetics; D009154:Mutation; D020543:Proteome / Q000032:analysis*; D017422:Sequence Analysis, DNA; D014260:Triclosan / Q000494:pharmacology*
chemical_list: D000891:Anti-Infective Agents, Local; D020543:Proteome; D014260:Triclosan
keywords: Genomics; Global gene response; Proteomics; RNA-Seq