title: Interaction Potency of Single-Walled Carbon Nanotubes with DNAs: A Novel Assay for Assessment of Hazard Risk.
abstract: Increasing use of single-walled carbon nanotubes (SWCNTs) necessitates a novel method for hazard risk assessment. In this work, we investigated the interaction of several types of commercial SWCNTs with single-stranded (ss) and double-stranded (ds) DNA oligonucleotides (20-mer and 20 bp). Based on the results achieved, we proposed a novel assay that employed the DNA interaction potency to assess the hazard risk of SWCNTs. It was found that SWCNTs in different sizes or different batches of the same product number of SWCNTs showed dramatically different potency of interaction with DNAs. In addition, the same SWCNTs also exerted strikingly different interaction potency with ss- versus ds- DNAs. The interaction rates of SWCNTs with DNAs were investigated, which could be utilized as the indicator of potential hazard for acute exposure. Compared to solid SWCNTs, the SWCNTs dispersed in liquid medium (2% sodium cholate solution) exhibited dramatically different interaction potency with DNAs. This indicates that the exposure medium may greatly influence the subsequent toxicity and hazard risk produced by SWCNTs. Based on the findings of dose-dependences and time-dependences from the interactions between SWCNTs and DNAs, a new chemistry based assay for hazard risk assessment of nanomaterials including SWCNTs has been presented.
authors.affiliation: Exposure and Biomonitoring Division, Environmental Health Science and Research Bureau, Environmental and Radiation Health Sciences Directorate, Healthy Environments and Consumer Safety Branch, Health Canada, Ottawa, Ontario, Canada.
authors.forename: Chunhe
authors.initials: C
authors.lastname: Yao
authors.affiliation: Exposure and Biomonitoring Division, Environmental Health Science and Research Bureau, Environmental and Radiation Health Sciences Directorate, Healthy Environments and Consumer Safety Branch, Health Canada, Ottawa, Ontario, Canada.
authors.forename: Cristina
authors.initials: C
authors.lastname: Carlisi
authors.affiliation: Department of Chemical Engineering, Department of Chemistry, and Waterloo Institute for Nanotechnology (WIN), University of Waterloo, Waterloo, Ontario, Canada.
authors.forename: Yuning
authors.initials: Y
authors.lastname: Li
authors.affiliation: Cooperative Wildlife Research Laboratory and Department of Zoology, Southern Illinois University, Carbondale, Illinois, United States.
authors.forename: Da
authors.initials: D
authors.lastname: Chen
authors.affiliation: Security and Disruptive Technologies, National Research Council of Canada, Ottawa, Ontario, Canada.
authors.forename: Jianfu
authors.initials: J
authors.lastname: Ding
authors.affiliation: Exposure and Biomonitoring Division, Environmental Health Science and Research Bureau, Environmental and Radiation Health Sciences Directorate, Healthy Environments and Consumer Safety Branch, Health Canada, Ottawa, Ontario, Canada.
authors.forename: Yong-Lai
authors.initials: YL
authors.lastname: Feng
mesh_terms: D004247:DNA / Q000378:metabolism*; D004277:DNA, Single-Stranded / Q000378:metabolism; D037742:Nanotubes, Carbon / Q000737:chemistry / Q000633:toxicity*; D018570:Risk Assessment; D018675:Toxicity Tests
chemical_list: D004277:DNA, Single-Stranded; D037742:Nanotubes, Carbon; D004247:DNA