title: Triosephosphate isomerase 1 suppresses growth, migration and invasion of hepatocellular carcinoma cells.
abstract: Metabolic dysregulation is one of the most common and recognizable features of cancer. Triosephosphate isomerase 1 (TPI1), which catalyzes the interconversion of dihydroxyacetone phosphate (DHAP) and d-glyceraldehyde-3-phosphate (G3P) during glycosis and gluconeogenesis, is a crucial enzyme in the carbohydrate metabolism. However, the biological function and mechanism of TPI1 in cancer remain largely unknown. In this study, we have found that TPI1 expression was greatly decreased in clinical HCC samples, positively correlated with overall survival, and negatively associated with histological differentiation, tumor size and organ metastasis. Forced expression of TPI1 in HCC cells inhibited cell growth, migration, and invasion in vitro. Consistently, knockdown of TPI1 by shRNA promoted cell growth, migration and invasion. Moreover, overexpression of TPI1 led to slowed tumor growth and decreased tumor weight in vivo. Furthermore, cell cycle arrest was induced by TPI1 overexpression. These phenotypes were associated with altered expression of β-catenin, Vimentin, P53, P27 and CyclinD1. Therefore, our data suggested that TPI1 functioned as a tumor suppressor in HCC and might serve as a potential therapeutic target for the treatment of HCC.
authors.affiliation: State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, China.
authors.forename: Hao
authors.initials: H
authors.lastname: Jiang
authors.affiliation: Institute of Nutrition Science, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China.
authors.forename: Ning
authors.initials: N
authors.lastname: Ma
authors.affiliation: Institute of Nutrition Science, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China.
authors.forename: Yurong
authors.initials: Y
authors.lastname: Shang
authors.affiliation: Department of General Surgery, Zhongshan Hospital Affiliated to Fudan University, Shanghai, China.
authors.forename: Wentao
authors.initials: W
authors.lastname: Zhou
authors.affiliation: Institute of Nutrition Science, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China.
authors.forename: Tianwei
authors.initials: T
authors.lastname: Chen
authors.affiliation: Institute of Nutrition Science, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China.
authors.forename: Dongxian
authors.initials: D
authors.lastname: Guan
authors.affiliation: Institute of Nutrition Science, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China.
authors.forename: Jingjing
authors.initials: J
authors.lastname: Li
authors.affiliation: Institute of Nutrition Science, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China.
authors.forename: Jingjing
authors.initials: J
authors.lastname: Wang
authors.affiliation: Institute of Nutrition Science, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China.
authors.forename: Erbin
authors.initials: E
authors.lastname: Zhang
authors.affiliation: Institute of Nutrition Science, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China.
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Feng
authors.affiliation: Institute of Nutrition Science, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China.
authors.forename: Fenfen
authors.initials: F
authors.lastname: Yin
authors.affiliation: Institute of Nutrition Science, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China.
authors.forename: Yanmei
authors.initials: Y
authors.lastname: Yuan
authors.affiliation: Institute of Nutrition Science, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China.
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Fang
authors.affiliation: Institute of Nutrition Science, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Qiu
authors.affiliation: Institute of Nutrition Science, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China. Electronic address: dxie@sibs.ac.cn.
authors.forename: Dong
authors.initials: D
authors.lastname: Xie
authors.affiliation: State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, China. Electronic address: dongzhiwei1234567@163.com.
authors.forename: Dongzhi
authors.initials: D
authors.lastname: Wei
mesh_terms: D000368:Aged; D000818:Animals; D063646:Carcinogenesis; D006528:Carcinoma, Hepatocellular / Q000378:metabolism*; D002465:Cell Movement; D049109:Cell Proliferation; D019938:Cyclin D1 / Q000378:metabolism; D018450:Disease Progression; D005260:Female; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007150:Immunohistochemistry; D008113:Liver Neoplasms / Q000378:metabolism*; D008297:Male; D051379:Mice; D008819:Mice, Nude; D008875:Middle Aged; D009361:Neoplasm Invasiveness; D009368:Neoplasm Transplantation; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism; D034741:RNA, Small Interfering / Q000378:metabolism; D014305:Triose-Phosphate Isomerase / Q000378:metabolism*; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism; D014746:Vimentin / Q000378:metabolism; D051176:beta Catenin / Q000378:metabolism
chemical_list: C530104:CCND1 protein, human; C495270:CTNNB1 protein, human; D018809:Proliferating Cell Nuclear Antigen; D034741:RNA, Small Interfering; C495901:TP53 protein, human; D016159:Tumor Suppressor Protein p53; D014746:Vimentin; D051176:beta Catenin; C413374:p27 antigen; D019938:Cyclin D1; D014305:Triose-Phosphate Isomerase
keywords: Cell cycle; Growth; HCC; Invasion; Migration; TPI1