title: High-density Electroencephalographic Acquisition in a Rodent Model Using Low-cost and Open-source Resources.
abstract: Advanced electroencephalographic analysis techniques requiring high spatial resolution, including electrical source imaging and measures of network connectivity, are applicable to an expanding variety of questions in neuroscience. Performing these kinds of analyses in a rodent model requires higher electrode density than traditional screw electrodes can accomplish. While higher-density electroencephalographic montages for rodents exist, they are of limited availability to most researchers, are not robust enough for repeated experiments over an extended period of time, or are limited to use in anesthetized rodents.1-3A proposed low-cost method for constructing a durable, high-count, transcranial electrode array, consisting of bilaterally implantable headpieces is investigated as a means to perform advanced electroencephalogram analyses in mice or rats. Procedures for headpiece fabrication and surgical implantation necessary to produce high signal to noise, low-impedance electroencephalographic and electromyographic signals are presented. While the methodology is useful in both rats and mice, this manuscript focuses on the more challenging implementation for the smaller mouse skull. Freely moving mice are only tethered to cables via a common adapter during recording. One version of this electrode system that includes 26 electroencephalographic channels and 4 electromyographic channels is described below.
authors.affiliation: Department of Anesthesiology and Critical Care, Perelman School of Medicine, University of Pennsylvania.
authors.forename: Andrzej Z
authors.initials: AZ
authors.lastname: Wasilczuk
authors.affiliation: Department of Anesthesiology and Critical Care, Perelman School of Medicine, University of Pennsylvania.
authors.forename: Alexander
authors.initials: A
authors.lastname: Proekt
authors.affiliation: Department of Anesthesiology and Critical Care, Perelman School of Medicine, University of Pennsylvania; Max.Kelz@uphs.upenn.edu.
authors.forename: Max B
authors.initials: MB
authors.lastname: Kelz
authors.affiliation: Department of Anesthesiology and Critical Care, Perelman School of Medicine, University of Pennsylvania.
authors.forename: Andrew R
authors.initials: AR
authors.lastname: McKinstry-Wu
mesh_terms: D000818:Animals; D004566:Electrodes; D004569:Electroencephalography; D051379:Mice; D051381:Rats; D012984:Software