title: Rats can acquire conditional fear of faint light leaking through the acrylic resin used to mount fiber optic cannulas.
abstract: Rodents are exquisitely sensitive to light and optogenetic behavioral experiments routinely introduce light-delivery materials into experimental situations, which raises the possibility that light could leak and influence behavioral performance. We examined whether rats respond to a faint diffusion of light, termed caplight, which emanated through the translucent dental acrylic resin used to affix deep-brain optical cannulas in place. Although rats did not display significant changes in locomotion or rearing to caplight in a darkened open field, they did acquire conditional fear via caplight-footshock pairings. These findings highlight the potential confounding influence of extraneous light emanating from light-delivery materials during optogenetic analyses.
authors.affiliation: Laboratoire de Physiopathologie des Maladies Psychiatriques, UMR_S894 Inserm, Centre de Psychiatrie et Neurosciences, Paris 75014, France.|Université Paris Descartes, Sorbonne Paris Cité, Paris 75006, France.
authors.forename: Adam
authors.initials: A
authors.lastname: Eckmier
authors.affiliation: INSP, CNRS7588, Université Pierre et Marie Curie-Paris 6, F-75252 Paris Cedex 05 92037, France.
authors.forename: Willy
authors.initials: W
authors.lastname: Daney de Marcillac
authors.affiliation: INSP, CNRS7588, Université Pierre et Marie Curie-Paris 6, F-75252 Paris Cedex 05 92037, France.
authors.forename: Agnès
authors.initials: A
authors.lastname: Maître
authors.affiliation: Laboratoire de Physiopathologie des Maladies Psychiatriques, UMR_S894 Inserm, Centre de Psychiatrie et Neurosciences, Paris 75014, France.|Université Paris Descartes, Sorbonne Paris Cité, Paris 75006, France.
authors.forename: Thérèse M
authors.identifier: 0000-0002-2468-1141
authors.initials: TM
authors.lastname: Jay
authors.affiliation: Psychology Department, College of Letters and Sciences, National University, La Jolla, California 92037, USA.
authors.forename: Matthew J
authors.initials: MJ
authors.lastname: Sanders
authors.affiliation: Laboratoire de Physiopathologie des Maladies Psychiatriques, UMR_S894 Inserm, Centre de Psychiatrie et Neurosciences, Paris 75014, France bp.godsil@gmail.com.|Université Paris Descartes, Sorbonne Paris Cité, Paris 75006, France.
authors.forename: Bill P
authors.identifier: 0000-0001-7248-4292
authors.initials: BP
authors.lastname: Godsil
mesh_terms: D000180:Acrylic Resins; D000818:Animals; D002408:Catheters, Indwelling; D003214:Conditioning, Classical; D004597:Electroshock; D005239:Fear; D046352:Freezing Reaction, Cataleptic; D008027:Light / Q000009:adverse effects; D008297:Male; D009043:Motor Activity; D055100:Optical Fibers; D062308:Optogenetics / Q000295:instrumentation*; D020318:Rats, Long-Evans; D017603:Signal Detection, Psychological
chemical_list: D000180:Acrylic Resins