title: Thermo-sensitive hydrogels combined with decellularised matrix deliver bFGF for the functional recovery of rats after a spinal cord injury.
abstract: Because of the short half-life, either systemic or local administration of bFGF shows significant drawbacks to spinal injury. In this study, an acellular spinal cord scaffold (ASC) was encapsulated in a thermo-sensitive hydrogel to overcome these limitations. The ASC was firstly prepared from the spinal cord of healthy rats and characterized by scanning electronic microscopy and immunohistochemical staining. bFGF could specifically complex with the ASC scaffold via electrostatic or receptor-mediated interactions. The bFGF-ASC complex was further encapsulated into a heparin modified poloxamer (HP) solution to prepare atemperature-sensitive hydrogel (bFGF-ASC-HP). bFGF release from the ASC-HP hydrogel was more slower than that from the bFGF-ASC complex alone. An in vitro cell survival study showed that the bFGF-ASC-HP hydrogel could more effectively promote the proliferation of PC12 cells than a bFGF solution, with an approximate 50% increase in the cell survival rate within 24 h (P < 0.05). Compared with the bFGF solution, bFGF-ASC-HP hydrogel displayed enhanced inhibition of glial scars and obviously improved the functional recovery of the SCI model rat through regeneration of nerve axons and the differentiation of the neural stem cells. In summary, an ASC-HP hydrogel might be a promising carrier to deliver bFGF to an injured spinal cord.
authors.affiliation: School of Pharmaceutical Sciences, Wenzhou Medical University, Wenzhou City, Zhejiang Province 325035, China.
authors.forename: He-Lin
authors.initials: HL
authors.lastname: Xu
authors.affiliation: School of Pharmaceutical Sciences, Wenzhou Medical University, Wenzhou City, Zhejiang Province 325035, China.
authors.forename: Fu-Rong
authors.initials: FR
authors.lastname: Tian
authors.affiliation: The Second Affiliated Hospital of Wenzhou Medical University, Wenzhou, 325000, China.
authors.forename: Cui-Tao
authors.initials: CT
authors.lastname: Lu
authors.affiliation: School of Pharmaceutical Sciences, Wenzhou Medical University, Wenzhou City, Zhejiang Province 325035, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Xu
authors.affiliation: School of Pharmaceutical Sciences, Wenzhou Medical University, Wenzhou City, Zhejiang Province 325035, China.
authors.forename: Zi-Liang
authors.initials: ZL
authors.lastname: Fan
authors.affiliation: School of Pharmaceutical Sciences, Wenzhou Medical University, Wenzhou City, Zhejiang Province 325035, China.
authors.forename: Jing-Jing
authors.initials: JJ
authors.lastname: Yang
authors.affiliation: School of Pharmaceutical Sciences, Wenzhou Medical University, Wenzhou City, Zhejiang Province 325035, China.
authors.forename: Pian-Pian
authors.initials: PP
authors.lastname: Chen
authors.affiliation: Biopharmaceutical R&D Center of Jinan University, Guangzhou, Guangdong 510000, China.
authors.forename: Ya-Dong
authors.initials: YD
authors.lastname: Huang
authors.affiliation: School of Pharmaceutical Sciences, Wenzhou Medical University, Wenzhou City, Zhejiang Province 325035, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Xiao
authors.affiliation: School of Pharmaceutical Sciences, Wenzhou Medical University, Wenzhou City, Zhejiang Province 325035, China.|The Second Affiliated Hospital of Wenzhou Medical University, Wenzhou, 325000, China.
authors.forename: Ying-Zheng
authors.initials: YZ
authors.lastname: Zhao
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D004195:Disease Models, Animal; D004339:Drug Compounding / Q000379:methods; D016503:Drug Delivery Systems / Q000379:methods*; D005260:Female; D016222:Fibroblast Growth Factor 2 / Q000378:metabolism / Q000493:pharmacokinetics / Q000494:pharmacology*; D006493:Heparin / Q000737:chemistry; D020100:Hydrogels / Q000737:chemistry*; D058953:Neural Stem Cells / Q000166:cytology / Q000187:drug effects / Q000502:physiology; D016716:PC12 Cells; D020442:Poloxamer / Q000737:chemistry; D051381:Rats; D017207:Rats, Sprague-Dawley; D020127:Recovery of Function / Q000187:drug effects*; D013119:Spinal Cord Injuries / Q000378:metabolism / Q000473:pathology / Q000628:therapy*; D013696:Temperature; D054457:Tissue Scaffolds
chemical_list: D020100:Hydrogels; D016222:Fibroblast Growth Factor 2; D020442:Poloxamer; D006493:Heparin