title: Draft genome and description of Orrella dioscoreae gen. nov. sp. nov., a new species of Alcaligenaceae isolated from leaf acumens of Dioscorea sansibarensis.
abstract: Dioscorea sansibarensis is a monocot species of inedible yam native of tropical Africa and Madagascar. Leaves of D. sansibarensis feature prominent acumen glands that are densely colonized by bacteria. We describe here the isolation and characterization of bacteria from leaf glands of D. sansibarensis specimens obtained from various botanical gardens. Phylogenetic and phenotypic characterizations indicate that the isolates belong to a novel genus and species of the Alcaligenaceae family for which we propose the name Orrella dioscoreae gen. nov. sp. nov., with type strain LMG 29303T[=CIP 111009T]. Strains isolated from different plants show limited phylogenetic and phenotypic diversity, indicating specific and tight association of the bacteria with the host plant. The analysis of the draft genome of strain LMG 29303Treveals features consistent with a recently evolved plant-associated lifestyle.
authors.affiliation: Laboratory of Microbiology, Faculty of Sciences, Ghent University, K. L. Ledeganckstraat 35, B-9000 Ghent, Belgium. Electronic address: aurelien.carlier@ugent.be.
authors.forename: Aurelien
authors.initials: A
authors.lastname: Carlier
authors.affiliation: Laboratory of Microbiology, Faculty of Sciences, Ghent University, K. L. Ledeganckstraat 35, B-9000 Ghent, Belgium.
authors.forename: Margo
authors.initials: M
authors.lastname: Cnockaert
authors.affiliation: Institute of Plant and Microbial Biology, University of Zurich, CH-8008 Zurich, Switzerland.
authors.forename: Linda
authors.initials: L
authors.lastname: Fehr
authors.affiliation: Laboratory of Microbiology, Faculty of Sciences, Ghent University, K. L. Ledeganckstraat 35, B-9000 Ghent, Belgium.
authors.forename: Peter
authors.initials: P
authors.lastname: Vandamme
authors.affiliation: Institute of Plant and Microbial Biology, University of Zurich, CH-8008 Zurich, Switzerland.
authors.forename: Leo
authors.initials: L
authors.lastname: Eberl
mesh_terms: D042401:Alcaligenaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D015373:Bacterial Typing Techniques; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry* / Q000235:genetics*; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D027742:Dioscorea / Q000382:microbiology*; D005227:Fatty Acids / Q000032:analysis; D016680:Genome, Bacterial; D008270:Madagascar; D010802:Phylogeny; D018515:Plant Leaves / Q000382:microbiology; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S
keywords: Alcaligenaceae; Plant-associated; symbiosis