title: Development of a multiplex taqMan real-time PCR assay for typing of Mycoplasma pneumoniae based on type-specific indels identified through whole genome sequencing.
abstract: We developed a multiplex real-time PCR assay for simultaneously detecting M. pneumoniae and typing into historically-defined P1 types. Typing was achieved based on the presence of short type-specific indels identified through whole genome sequencing. This assay was 100% specific compared to existing methods and may be useful during epidemiologic investigations.
authors.affiliation: Division of Bacterial Diseases, Centers for Disease Control and Prevention, Atlanta, GA, USA.
authors.forename: Bernard J
authors.initials: BJ
authors.lastname: Wolff
authors.affiliation: Division of Bacterial Diseases, Centers for Disease Control and Prevention, Atlanta, GA, USA.
authors.forename: Alvaro J
authors.initials: AJ
authors.lastname: Benitez
authors.affiliation: Division of Bacterial Diseases, Centers for Disease Control and Prevention, Atlanta, GA, USA.
authors.forename: Heta P
authors.initials: HP
authors.lastname: Desai
authors.affiliation: Division of Bacterial Diseases, Centers for Disease Control and Prevention, Atlanta, GA, USA.
authors.forename: Shatavia S
authors.initials: SS
authors.lastname: Morrison
authors.affiliation: Division of Bacterial Diseases, Centers for Disease Control and Prevention, Atlanta, GA, USA.
authors.forename: Maureen H
authors.initials: MH
authors.lastname: Diaz
authors.affiliation: Division of Bacterial Diseases, Centers for Disease Control and Prevention, Atlanta, GA, USA. Electronic address: jwinchell@cdc.gov.
authors.forename: Jonas M
authors.initials: JM
authors.lastname: Winchell
mesh_terms: D001483:Base Sequence; D004269:DNA, Bacterial / Q000235:genetics; D016680:Genome, Bacterial / Q000235:genetics*; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods*; D006801:Humans; D058889:Molecular Typing / Q000379:methods*; D009177:Mycoplasma pneumoniae / Q000145:classification / Q000235:genetics*; D011019:Pneumonia, Mycoplasma / Q000175:diagnosis* / Q000382:microbiology; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA / Q000379:methods*
chemical_list: D004269:DNA, Bacterial
keywords: M. pneumoniae; Next generation sequencing; P1 typing; Real-time PCR