title: Detection of the biofilm component polysaccharide intercellular adhesin in Staphylococcus aureus infected cow udders.
abstract: Biofilms are communities of microorganisms embedded in a self-produced extracellular matrix made up of polymeric substances. They reduce the effects of antibiotics and allow the microorganisms to evade the innate immune system. This can lead to persistent or recurrent infections. In dairy cow herds, mastitis is a serious problem. The present study aimed to investigate the occurrence of biofilms in the udders of dairy cows infected with Staphylococcus (S.) aureus, because biofilms may affect the response to treatment of bovine mastitis. Immunofluorescence staining of polysaccharide intercellular adhesin (PIA), a component of S. aureus biofilms, was carried out based on swabs taken from different areas of S. aureus infected udders. We were able to demonstrate the presence of PIA in S. aureus infected bovine udders. However, the applied method is invasive and therefore only really suitable for scientific research and not for clinical diagnosis.
authors.affiliation: Faculty II, Department of Microbiology, University of Applied Sciences and Arts Hannover, Heisterbergallee 12, 30453 Hannover, Germany.
authors.forename: Sarah
authors.initials: S
authors.lastname: Schönborn
authors.affiliation: Faculty II, Department of Microbiology, University of Applied Sciences and Arts Hannover, Heisterbergallee 12, 30453 Hannover, Germany. Electronic address: volker.kroemker@hs-hannover.de.
authors.forename: Volker
authors.initials: V
authors.lastname: Krömker
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D018441:Biofilms / Q000254:growth & development*; D002417:Cattle; D005260:Female; D008321:Mammary Glands, Animal / Q000382:microbiology; D008414:Mastitis, Bovine / Q000382:microbiology*; D011135:Polysaccharides, Bacterial / Q000235:genetics / Q000378:metabolism*; D013203:Staphylococcal Infections / Q000382:microbiology / Q000662:veterinary*; D013211:Staphylococcus aureus / Q000187:drug effects / Q000254:growth & development / Q000502:physiology*; D047071:beta-Glucans
chemical_list: D000900:Anti-Bacterial Agents; D011135:Polysaccharides, Bacterial; D047071:beta-Glucans; C505465:poly-N-acetyl-1-6-glucosamine; C113579:polysaccharide intercellular adhesin
keywords: Biofilm; Mastitis; Poly-N-acetyl-β-(1,6)-glucosamine (PNAG); Polysaccharide intercellular adhesin (PIA); Staphylococcus aureus