title: A human microprotein that interacts with the mRNA decapping complex.
abstract: Proteomic detection of non-annotated microproteins indicates the translation of hundreds of small open reading frames (smORFs) in human cells, but whether these microproteins are functional or not is unknown. Here, we report the discovery and characterization of a 7-kDa human microprotein we named non-annotated P-body dissociating polypeptide (NoBody). NoBody interacts with mRNA decapping proteins, which remove the 5' cap from mRNAs to promote 5'-to-3' decay. Decapping proteins participate in mRNA turnover and nonsense-mediated decay (NMD). NoBody localizes to mRNA-decay-associated RNA-protein granules called P-bodies. Modulation of NoBody levels reveals that its abundance is anticorrelated with cellular P-body numbers and alters the steady-state levels of a cellular NMD substrate. These results implicate NoBody as a novel component of the mRNA decapping complex and demonstrate potential functionality of a newly discovered microprotein.
authors.affiliation: Department of Chemistry and Chemical Biology, Chemical Biology Institute, Yale University, New Haven, Connecticut, USA.
authors.forename: Nadia G
authors.initials: NG
authors.lastname: D'Lima
authors.affiliation: Clayton Foundation Peptide Biology Lab, Helmsley Center for Genomic Medicine, Salk Institute for Biological Studies, La Jolla, California, USA.
authors.forename: Jiao
authors.initials: J
authors.lastname: Ma
authors.affiliation: Department of Chemistry and Chemical Biology, Chemical Biology Institute, Yale University, New Haven, Connecticut, USA.
authors.forename: Lauren
authors.initials: L
authors.lastname: Winkler
authors.affiliation: Clayton Foundation Peptide Biology Lab, Helmsley Center for Genomic Medicine, Salk Institute for Biological Studies, La Jolla, California, USA.
authors.forename: Qian
authors.initials: Q
authors.lastname: Chu
authors.affiliation: Department of Chemistry, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA.
authors.forename: Ken H
authors.initials: KH
authors.lastname: Loh
authors.affiliation: Division of Biological Sciences, University of California, San Diego, La Jolla, California, USA.
authors.forename: Elizabeth O
authors.initials: EO
authors.lastname: Corpuz
authors.affiliation: FAS Center for Systems Biology, Harvard University, Cambridge, Massachusetts, USA.
authors.forename: Bogdan A
authors.initials: BA
authors.lastname: Budnik
authors.affiliation: Division of Biological Sciences, University of California, San Diego, La Jolla, California, USA.
authors.forename: Jens
authors.initials: J
authors.lastname: Lykke-Andersen
authors.affiliation: Clayton Foundation Peptide Biology Lab, Helmsley Center for Genomic Medicine, Salk Institute for Biological Studies, La Jolla, California, USA.
authors.forename: Alan
authors.initials: A
authors.lastname: Saghatelian
authors.affiliation: Department of Chemistry and Chemical Biology, Chemical Biology Institute, Yale University, New Haven, Connecticut, USA.
authors.forename: Sarah A
authors.initials: SA
authors.lastname: Slavoff
mesh_terms: D000818:Animals; D019556:COS Cells; D002352:Carrier Proteins / Q000378:metabolism*; D002478:Cells, Cultured; D002522:Chlorocebus aethiops; D004722:Endoribonucleases / Q000737:chemistry* / Q000378:metabolism*; D006801:Humans; D012315:RNA Caps / Q000378:metabolism; D012333:RNA, Messenger / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*
chemical_list: D002352:Carrier Proteins; D012315:RNA Caps; D012333:RNA, Messenger; C030052:mRNA decapping enzymes; C000618379:non-annotated P-body dissociating polypeptide, human; D004722:Endoribonucleases