title: One-Step PCR Detection ofSalmonellaPullorum/Gallinarum Using a Novel Target: The Flagellar Biosynthesis GeneflhB.
abstract: Salmonella entericaserovar Pullorum/Gallinarum is an important infectious pathogen that has caused widespread problems for chicken industry. TraditionalSalmonellaserotyping is an expensive and time-consuming process. In this study, we developed a rapid one-step polymerase chain reaction (PCR) method to identifyS.Pullorum/Gallinarum. The PCR-based assay focuses onflhB, which shows a deficient region only inS.Pullorum/Gallinarum, compared with that of other serovars. The specificity and sensitivity of the PCR system were evaluated. The developed PCR method could identifyS.Pullorum/Gallinarum from 27 differentSalmonellaserovars and eight non-Salmonellapathogens. The minimum limit of DNA and the lowest number of cells ofS.Pullorum for the PCR detection were no less than 5.85 pg/μL and 10 CFU, respectively. The method was applied to the analysis ofSalmonellastrains isolated from the chicken farm. The PCR-based testing results of the farm isolates were in concordance with those obtained using traditional serotyping method. This newly developed PCR-based system could be used to accurately screen for the presence ofS.Pullorum/Gallinarum, and support traditional serotyping methods, especially in high-throughput screening situations.
authors.affiliation: Jiangsu Key Laboratory of Zoonosis, Yangzhou UniversityYangzhou, China; Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and ZoonosesYangzhou, China; Joint International Research Laboratory of Agriculture and Agri-product Safety of the Ministry of EducationYangzhou, China.
authors.forename: Dan
authors.initials: D
authors.lastname: Xiong
authors.affiliation: Jiangsu Key Laboratory of Zoonosis, Yangzhou UniversityYangzhou, China; Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and ZoonosesYangzhou, China; Joint International Research Laboratory of Agriculture and Agri-product Safety of the Ministry of EducationYangzhou, China.
authors.forename: Li
authors.initials: L
authors.lastname: Song
authors.affiliation: Jiangsu Key Laboratory of Zoonosis, Yangzhou UniversityYangzhou, China; Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and ZoonosesYangzhou, China; Joint International Research Laboratory of Agriculture and Agri-product Safety of the Ministry of EducationYangzhou, China.
authors.forename: Shizhong
authors.initials: S
authors.lastname: Geng
authors.affiliation: Jiangsu Key Laboratory of Zoonosis, Yangzhou UniversityYangzhou, China; Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and ZoonosesYangzhou, China; Joint International Research Laboratory of Agriculture and Agri-product Safety of the Ministry of EducationYangzhou, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Tao
authors.affiliation: Jiangsu Key Laboratory of Zoonosis, Yangzhou UniversityYangzhou, China; Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and ZoonosesYangzhou, China; Joint International Research Laboratory of Agriculture and Agri-product Safety of the Ministry of EducationYangzhou, China.
authors.forename: Shumin
authors.initials: S
authors.lastname: An
authors.affiliation: Jiangsu Key Laboratory of Zoonosis, Yangzhou UniversityYangzhou, China; Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and ZoonosesYangzhou, China; Joint International Research Laboratory of Agriculture and Agri-product Safety of the Ministry of EducationYangzhou, China.
authors.forename: Zhiming
authors.initials: Z
authors.lastname: Pan
authors.affiliation: Jiangsu Key Laboratory of Zoonosis, Yangzhou UniversityYangzhou, China; Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and ZoonosesYangzhou, China; Joint International Research Laboratory of Agriculture and Agri-product Safety of the Ministry of EducationYangzhou, China.
authors.forename: Xinan
authors.initials: X
authors.lastname: Jiao
keywords: PCR detection; Salmonella Pullorum/Gallinarum; chicken farm; flhB; one-step