title: In Vivo Biotransformation of the Fusion Protein Tetranectin-Apolipoprotein A1 Analyzed by Ligand-Binding Mass Spectrometry Combined with Quantitation by ELISA.
abstract: The in vivo biotransformation of a novel fusion protein tetranectin/apolipoprotein A1 (TN-ApoA1) was investigated by ligand-binding mass spectrometry (LB-MS) in support of enzyme-linked immunosorbent assays (ELISA). The main focus was on catabolites formed by proteolysis of the fusion protein in rabbit following intravenous administration of lipidated TN-ApoA1. The drug and its catabolites were isolated from rabbit plasma by immunocapture with a monoclonal antibody (mAb) binding to the fusion region of TN-ApoA1. The captured drug and catabolites were released from the streptavidin-coated magnetic beads, separated by monolithic RP capillary HPLC, and online detected by high-resolution mass spectrometry. In addition, the same extract was digested with LysN to confirm or further narrow down the structure of the found catabolites. Two pharmacologically active catabolites were identified with conserved fusion region. The major catabolite [3-285] was formed by truncation of AP at the N-terminus and the minor catabolite [29-270] by truncations of either side of the TN-ApoA1 sequence. Since the ELISA determined the sum of TN-ApoA1, along with its two main catabolites, the individual PK profiles of all three components could be derived by applying their mass peak composition for each sampling point. Parent drug accounted for 25% of drug-related material, whereas that of the catabolites [3-285] and [29-270] accounted for 66% and 9%, respectively. This result could be obtained without catabolite specific ELISAs or quantitative LC-MS assays. It was also confirmed that all relevant functional molecules of TN-ApoA1 in the plasma samples were quantified by the ELISA, which provided a good relationship for pharmacokinetic/pharmacodynamic evaluations.
authors.affiliation: Roche Pharma Research and Early Development, Roche Innovation Center Basel, F. Hoffmann-La Roche Ltd , Grenzacherstrasse 124, 4070 Basel, Switzerland.
authors.forename: Manfred
authors.initials: M
authors.lastname: Zell
authors.affiliation: Roche Pharma Research and Early Development, Roche Innovation Center Basel, F. Hoffmann-La Roche Ltd , Grenzacherstrasse 124, 4070 Basel, Switzerland.
authors.forename: Christophe
authors.initials: C
authors.lastname: Husser
authors.affiliation: Roche Pharma Research and Early Development, Roche Innovation Center Munich, Roche Diagnostics GmbH , Nonnenwald 2, 82377 Penzberg, Germany.
authors.forename: Roland F
authors.initials: RF
authors.lastname: Staack
authors.affiliation: Roche Pharma Research and Early Development, Roche Innovation Center Munich, Roche Diagnostics GmbH , Nonnenwald 2, 82377 Penzberg, Germany.
authors.forename: Gregor
authors.initials: G
authors.lastname: Jordan
authors.affiliation: Roche Pharma Research and Early Development, Roche Innovation Center Basel, F. Hoffmann-La Roche Ltd , Grenzacherstrasse 124, 4070 Basel, Switzerland.
authors.forename: Wolfgang F
authors.initials: WF
authors.lastname: Richter
authors.affiliation: Roche Pharma Research and Early Development, Roche Innovation Center Basel, F. Hoffmann-La Roche Ltd , Grenzacherstrasse 124, 4070 Basel, Switzerland.
authors.forename: Simone
authors.initials: S
authors.lastname: Schadt
authors.affiliation: Roche Pharma Research and Early Development, Roche Innovation Center Basel, F. Hoffmann-La Roche Ltd , Grenzacherstrasse 124, 4070 Basel, Switzerland.
authors.forename: Axel
authors.initials: A
authors.lastname: Pähler
mesh_terms: D016632:Apolipoprotein A-I / Q000032:analysis*; D001665:Binding Sites; D001711:Biotransformation; D004797:Enzyme-Linked Immunosorbent Assay; D037181:Lectins, C-Type / Q000032:analysis*; D008024:Ligands; D013058:Mass Spectrometry
chemical_list: D016632:Apolipoprotein A-I; D037181:Lectins, C-Type; D008024:Ligands; C048648:tetranectin