title: FYCO1 and autophagy control the integrity of the haploid male germ cell-specific RNP granules.
abstract: Ribonucleoprotein (RNP) granules play a major role in compartmentalizing cytoplasmic RNA regulation. Haploid round spermatids that have exceptionally diverse transcriptomes are characterized by a unique germ cell-specific RNP granule, the chromatoid body (CB). The CB shares many characteristics with somatic RNP granules but also has germline-specific features. The CB appears to be a central structure in PIWI-interacting RNA (piRNA)-targeted RNA regulation. Here, we identified a novel CB component, FYCO1, which is involved in the intracellular transport of autophagic vesicles in somatic cells. We demonstrated that the CB is associated with autophagic activity. Induction of autophagy leads to the recruitment of lysosomal vesicles onto the CB in a FYCO1-dependent manner as demonstrated by the analysis of a germ cell-specific Fyco1 conditional knockout mouse model. Furthermore, in the absence of FYCO1, the integrity of the CB was affected and the CB was fragmented. Our results suggest that RNP granule homeostasis is regulated by FYCO1-mediated autophagy.
authors.affiliation: a Institute of Biomedicine, Department of Physiology , University of Turku , Turku , Finland.|b Department of Cellular and Molecular Biology , Faculty of Medicine, University of Ottawa , Ottawa , ON , Canada.
authors.forename: Matteo
authors.initials: M
authors.lastname: Da Ros
authors.affiliation: a Institute of Biomedicine, Department of Physiology , University of Turku , Turku , Finland.
authors.forename: Tiina
authors.initials: T
authors.lastname: Lehtiniemi
authors.affiliation: a Institute of Biomedicine, Department of Physiology , University of Turku , Turku , Finland.
authors.forename: Opeyemi
authors.initials: O
authors.lastname: Olotu
authors.affiliation: c Natural Resources Institute Finland (Luke), Green Technology , Jokioinen , Finland.
authors.forename: Daniel
authors.initials: D
authors.lastname: Fischer
authors.affiliation: a Institute of Biomedicine, Department of Physiology , University of Turku , Turku , Finland.|d Turku Center for Disease Modeling, University of Turku , Turku , Finland.
authors.forename: Fu-Ping
authors.initials: FP
authors.lastname: Zhang
authors.affiliation: e Electron Microscopy Unit, Institute of Biotechnology, University of Helsinki , Helsinki , Finland.
authors.forename: Helena
authors.initials: H
authors.lastname: Vihinen
authors.affiliation: e Electron Microscopy Unit, Institute of Biotechnology, University of Helsinki , Helsinki , Finland.
authors.forename: Eija
authors.initials: E
authors.lastname: Jokitalo
authors.affiliation: c Natural Resources Institute Finland (Luke), Green Technology , Jokioinen , Finland.
authors.forename: Anu
authors.initials: A
authors.lastname: Sironen
authors.affiliation: a Institute of Biomedicine, Department of Physiology , University of Turku , Turku , Finland.|f Department of Pediatrics , University of Turku and Turku University Hospital , Turku , Finland.
authors.forename: Jorma
authors.initials: J
authors.lastname: Toppari
authors.affiliation: a Institute of Biomedicine, Department of Physiology , University of Turku , Turku , Finland.
authors.forename: Noora
authors.initials: N
authors.lastname: Kotaja
mesh_terms: D000818:Animals; D060565:Argonaute Proteins / Q000235:genetics / Q000378:metabolism; D000071182:Autophagosomes / Q000378:metabolism / Q000648:ultrastructure; D001343:Autophagy; D003594:Cytoplasmic Granules / Q000378:metabolism* / Q000648:ultrastructure; D003598:Cytoskeletal Proteins; D004268:DNA-Binding Proteins / Q000378:metabolism*; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D063990:Gene Ontology; D006238:Haploidy; D008247:Lysosomes / Q000378:metabolism / Q000648:ultrastructure; D008297:Male; D018345:Mice, Knockout; D008870:Microtubules / Q000378:metabolism / Q000648:ultrastructure; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D009928:Organ Specificity; D019869:Phosphatidylinositol 3-Kinases / Q000378:metabolism; D000081082:Phosphoinositide-3 Kinase Inhibitors; D011485:Protein Binding; D021381:Protein Transport; D012261:Ribonucleoproteins / Q000378:metabolism*; D013087:Spermatids / Q000378:metabolism / Q000648:ultrastructure; D013091:Spermatogenesis; D013094:Spermatozoa / Q000166:cytology* / Q000378:metabolism* / Q000648:ultrastructure; D013737:Testis / Q000378:metabolism
chemical_list: D060565:Argonaute Proteins; D003598:Cytoskeletal Proteins; D004268:DNA-Binding Proteins; D009419:Nerve Tissue Proteins; D019869:Phosphatidylinositol 3-Kinases; D000081082:Phosphoinositide-3 Kinase Inhibitors; D012261:Ribonucleoproteins; C448521:Piwil2 protein, mouse; C000592360:Rufy3 protein, mouse
keywords: FYCO1; RNP granule; autophagy; chromatoid body; germ cell; knockout; lysosome; posttranscriptional; spermatogenesis; vesicle