title: Resveratrol Inhibits Proliferation, Invasion, and Epithelial-Mesenchymal Transition by Increasing miR-200c Expression in HCT-116 Colorectal Cancer Cells.
abstract: colorectal cancer (CRC) is one of the most common malignancies, associated with high rates of relapse. A notable challenge in treatment is low response rate to current therapies for advanced CRC. The miR-200c plays an essential role in tumor suppression by inhibiting epithelial-mesenchymal transition (EMT). Resveratrol, a natural compound found in red wine, reveals anti-cancer properties in several types of cancers such as CRC. The aim of current study was to evaluate the effects of resveratrol on proliferation, apoptosis, and invasion of HCT-116 cells and also expression of EMT-related genes in presences or absence of miR-200c.
  
  
  
  the effect of resveratrol on viability was examined by MTT assay. LNA-anti-miR-200c transfection of HCT-116 cells was carried out in a time dependent manner. Then, the expression of miR-200c and EMT-related genes were quantified by qRT-PCR. Further, expression of EMT-related proteins, apoptosis, and invasion were analyzed by Western blot, Annexin V/PI staining and scratch test, respectively.
  
  
  
  resveratrol could significantly inhibit viability of HCT-116 cells. LNA-anti-miR-200c suppressed the endogenous miR-200c in transfected cells compared with the control. qRT-PCR and Western blot analysis of LNA-anti-miR-200c transfected cells revealed a considerable increase in vimentin and ZEB-1 expression, with a concomitant reduction in E-cadherin expression level. Migration of HCT-116 cells increased, and apoptosis significantly reduced in transfected cells. While, resveratrol could entirely reverse these changes by modulation of miR-200c expression.
  
  
  
  our findings revealed a major role of resveratrol in apoptosis, invasion, and switching of EMT to MET phenotype through upregulation of miR-200c in CRC. J. Cell. Biochem. 118: 1547-1555, 2017. © 2016 Wiley Periodicals, Inc.
authors.affiliation: Research Center for Molecular Medicine, Hamadan University of Medical Sciences, Hamadan, Iran.
authors.forename: Fatemeh
authors.initials: F
authors.lastname: Karimi Dermani
authors.affiliation: Research Center for Molecular Medicine, Hamadan University of Medical Sciences, Hamadan, Iran.
authors.forename: Massoud
authors.initials: M
authors.lastname: Saidijam
authors.affiliation: Research Center for Molecular Medicine, Hamadan University of Medical Sciences, Hamadan, Iran.
authors.forename: Razieh
authors.initials: R
authors.lastname: Amini
authors.affiliation: Research Center for Molecular Medicine, Hamadan University of Medical Sciences, Hamadan, Iran.
authors.forename: Ali
authors.initials: A
authors.lastname: Mahdavinezhad
authors.affiliation: Research Center for Molecular Medicine, Hamadan University of Medical Sciences, Hamadan, Iran.
authors.forename: Korosh
authors.initials: K
authors.lastname: Heydari
authors.affiliation: Research Center for Molecular Medicine, Hamadan University of Medical Sciences, Hamadan, Iran.|Endometrium and Endometriosis Research Center, Hamadan University of Medical Sciences, Hamadan, Iran.
authors.forename: Rezvan
authors.initials: R
authors.lastname: Najafi
mesh_terms: D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D015179:Colorectal Neoplasms / Q000188:drug therapy / Q000235:genetics*; D058750:Epithelial-Mesenchymal Transition / Q000187:drug effects; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D045325:HCT116 Cells; D006801:Humans; D035683:MicroRNAs / Q000235:genetics*; D009361:Neoplasm Invasiveness; D000077185:Resveratrol; D013267:Stilbenes / Q000494:pharmacology*; D015854:Up-Regulation
chemical_list: C533896:MIRN200 microRNA, human; D035683:MicroRNAs; D013267:Stilbenes; D000077185:Resveratrol
keywords: COLORECTAL CANCER; EPITHELIAL-MESENCHYMAL TRANSITION; RESVERATROL; miR-200c