title: Highly Sensitive and Quick Detection of Acute Myocardial Infarction Biomarkers Using In2O3Nanoribbon Biosensors Fabricated Using Shadow Masks.
abstract: We demonstrate a scalable and facile lithography-free method for fabricating highly uniform and sensitive In2O3nanoribbon biosensor arrays. Fabrication with shadow masks as the patterning method instead of conventional lithography provides low-cost, time-efficient, and high-throughput In2O3nanoribbon biosensors without photoresist contamination. Combined with electronic enzyme-linked immunosorbent assay for signal amplification, the In2O3nanoribbon biosensor arrays are optimized for early, quick, and quantitative detection of cardiac biomarkers in diagnosis of acute myocardial infarction (AMI). Cardiac troponin I (cTnI), creatine kinase MB (CK-MB), and B-type natriuretic peptide (BNP) are commonly associated with heart attack and heart failure and have been selected as the target biomarkers here. Our approach can detect label-free biomarkers for concentrations down to 1 pg/mL (cTnI), 0.1 ng/mL (CK-MB), and 10 pg/mL (BNP), all of which are much lower than clinically relevant cutoff concentrations. The sample collection to result time is only 45 min, and we have further demonstrated the reusability of the sensors. With the demonstrated sensitivity, quick turnaround time, and reusability, the In2O3nanoribbon biosensors have shown great potential toward clinical tests for early and quick diagnosis of AMI.
authors.affiliation: Mork Family Department of Chemical Engineering and Materials Science, ‡Ming Hsieh Department of Electrical Engineering, and §Department of Chemistry, University of Southern California , Los Angeles, California 90089, United States.
authors.forename: Qingzhou
authors.initials: Q
authors.lastname: Liu
authors.affiliation: Mork Family Department of Chemical Engineering and Materials Science, ‡Ming Hsieh Department of Electrical Engineering, and §Department of Chemistry, University of Southern California , Los Angeles, California 90089, United States.
authors.forename: Noppadol
authors.initials: N
authors.lastname: Aroonyadet
authors.affiliation: Mork Family Department of Chemical Engineering and Materials Science, ‡Ming Hsieh Department of Electrical Engineering, and §Department of Chemistry, University of Southern California , Los Angeles, California 90089, United States.
authors.forename: Yan
authors.initials: Y
authors.lastname: Song
authors.affiliation: Mork Family Department of Chemical Engineering and Materials Science, ‡Ming Hsieh Department of Electrical Engineering, and §Department of Chemistry, University of Southern California , Los Angeles, California 90089, United States.
authors.forename: Xiaoli
authors.initials: X
authors.lastname: Wang
authors.affiliation: Mork Family Department of Chemical Engineering and Materials Science, ‡Ming Hsieh Department of Electrical Engineering, and §Department of Chemistry, University of Southern California , Los Angeles, California 90089, United States.
authors.forename: Xuan
authors.initials: X
authors.lastname: Cao
authors.affiliation: Mork Family Department of Chemical Engineering and Materials Science, ‡Ming Hsieh Department of Electrical Engineering, and §Department of Chemistry, University of Southern California , Los Angeles, California 90089, United States.
authors.forename: Yihang
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Mork Family Department of Chemical Engineering and Materials Science, ‡Ming Hsieh Department of Electrical Engineering, and §Department of Chemistry, University of Southern California , Los Angeles, California 90089, United States.
authors.forename: Sen
authors.initials: S
authors.lastname: Cong
authors.affiliation: Mork Family Department of Chemical Engineering and Materials Science, ‡Ming Hsieh Department of Electrical Engineering, and §Department of Chemistry, University of Southern California , Los Angeles, California 90089, United States.
authors.forename: Fanqi
authors.initials: F
authors.lastname: Wu
authors.affiliation: Mork Family Department of Chemical Engineering and Materials Science, ‡Ming Hsieh Department of Electrical Engineering, and §Department of Chemistry, University of Southern California , Los Angeles, California 90089, United States.
authors.forename: Mark E
authors.initials: ME
authors.lastname: Thompson
authors.affiliation: Mork Family Department of Chemical Engineering and Materials Science, ‡Ming Hsieh Department of Electrical Engineering, and §Department of Chemistry, University of Southern California , Los Angeles, California 90089, United States.
authors.forename: Chongwu
authors.initials: C
authors.lastname: Zhou
mesh_terms: D015415:Biomarkers; D015374:Biosensing Techniques; D006801:Humans; D009203:Myocardial Infarction / Q000175:diagnosis*; D037742:Nanotubes, Carbon; D012680:Sensitivity and Specificity; D019210:Troponin I / Q000032:analysis
chemical_list: D015415:Biomarkers; D037742:Nanotubes, Carbon; D019210:Troponin I
keywords: acute myocardial infarction diagnosis; biosensor; electronic ELISA; field-effect transistor; indium oxide semiconductor; shadow mask fabrication