title: γ-H2AX/53BP1/pKAP-1 foci and their linear tracks induced by in vitro exposure to radon and its progeny in human peripheral blood lymphocytes.
abstract: The biodosimetric information is critical for evaluating the human health hazards caused by radon and its progeny. Here, we demonstrated that the formation of phosphorylated histone variant H2AX (γ-H2AX), p53-binding protein 1 (53BP1) and phosphorylated KRAB-associated protein 1 (pKAP-1) foci and their linear tracks in human peripheral blood lymphocytes (HPBLs) in vitro exposed to radon and its progeny were dependent on the cumulative absorbed dose of radon exposure but was unrelated to the concentration of radon. Among them, γ-H2AX foci and its linear tracks were the most sensitive indicators with the lowest estimable cumulative absorbed dose of 1.74 mGy from their linear dose-response curves and sustained for 12 h after termination of radon exposure. In addition, three types of foci showed an overdispersed non-Poisson distribution in HPBLs. The ratios of pKAP-1/γ-H2AX foci co-localization, 53BP1/γ-H2AX foci co-localization and 53BP1/pKAP-1 foci co-localization were significantly increased in HPBLs exposed to radon while they were unrelated to the cumulative dose of radon exposure, suggesting that γ-H2AX, pKAP-1 and 53BP1 play an important role in the repair of heterochromatic double-strand breaks. Altogether, our findings provide an experimental basis for estimating the biological dose of internal α-particle irradiation from radon and its progeny exposure in humans.
authors.affiliation: Department of Radiation Biology, Institute of Radiation Medicine, Fudan University, Shanghai, 200032, China.|Department of Radiology, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai, 200233, China.
authors.forename: Defang
authors.initials: D
authors.lastname: Ding
authors.affiliation: Department of Radiation Biology, Institute of Radiation Medicine, Fudan University, Shanghai, 200032, China.
authors.forename: Yaping
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Radiation Biology, Institute of Radiation Medicine, Fudan University, Shanghai, 200032, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Wang
authors.affiliation: Institute of Modern Physics, Department of Nuclear Science and Technology, Fudan University, Shanghai, 200433, China.
authors.forename: Xufei
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Radiological Health, Institute of Radiation Medicine, Fudan University, Shanghai, 200032, China.
authors.forename: Dunhuang
authors.initials: D
authors.lastname: Fan
authors.affiliation: Division of Ionizing Radiation Measurement Technology, Shanghai Institute of Measurement and Testing Technology, Shanghai, 201203, China.
authors.forename: Linfeng
authors.initials: L
authors.lastname: He
authors.affiliation: Department of Radiation Biology, Institute of Radiation Medicine, Fudan University, Shanghai, 200032, China.
authors.forename: Xuxia
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Department of Radiation Biology, Institute of Radiation Medicine, Fudan University, Shanghai, 200032, China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Gao
authors.affiliation: Department of Radiation Biology, Institute of Radiation Medicine, Fudan University, Shanghai, 200032, China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Li
authors.affiliation: Department of Radiation Biology, Institute of Radiation Medicine, Fudan University, Shanghai, 200032, China.
authors.forename: Honghong
authors.initials: H
authors.lastname: Chen
mesh_terms: D000396:Air Pollutants, Radioactive / Q000494:pharmacology*; D004247:DNA / Q000235:genetics* / Q000378:metabolism; D053903:DNA Breaks, Double-Stranded; D004260:DNA Repair; D004307:Dose-Response Relationship, Radiation; D006657:Histones / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000378:metabolism / Q000528:radiation effects; D010766:Phosphorylation / Q000528:radiation effects; D061251:Primary Cell Culture; D011874:Radiometry; D011886:Radon / Q000494:pharmacology*; D000076183:Tripartite Motif-Containing Protein 28 / Q000235:genetics* / Q000378:metabolism; D000071857:Tumor Suppressor p53-Binding Protein 1 / Q000235:genetics* / Q000378:metabolism
chemical_list: D000396:Air Pollutants, Radioactive; C468783:H2AX protein, human; D006657:Histones; C498301:TP53BP1 protein, human; D000071857:Tumor Suppressor p53-Binding Protein 1; D004247:DNA; C101116:TRIM28 protein, human; D000076183:Tripartite Motif-Containing Protein 28; D011886:Radon