title: Antioxidant and hemolytic activities, and effects in rat cutaneous wound healing of a novel polysaccharide from fenugreek (Trigonella foenum-graecum) seeds.
abstract: The aim of this work was to evaluate the antioxidant and hemolytic activities as well as the in vivo wound healing performance of a novel polysaccharide (FWEP) extracted from fenugreek (Trigonella foenum-graecum) seeds. The antioxidant activity was evaluated in vivo and in vitro using various assays. Results showed that FWEP exhibited strong antioxidant activities but no hemolytic activity was observed towards bovine erythrocytes. The application of FWEP hydrogel on the wound site in a rat model enhanced significantly wound healing activity and accelerated the wound closure after 14days of wound induction. Histological examination also demonstrated fully re-epithelialized wound with a complete epidermal regeneration. Altogether, these evidences demonstrated that FWEP had strong wound healing potential presumably achieved through its antioxidant activities.
authors.affiliation: Laboratory of Enzyme Engineering and Microbiology, National School of Engineering of Sfax, University of Sfax, P.O. 1173-3038, Sfax, Tunisia.
authors.forename: Naourez
authors.initials: N
authors.lastname: Ktari
authors.affiliation: Laboratory of Microorganisms and Biomolecules (LMB), Centre of Biotechnology of Sfax, Road of Sidi Mansour Km 6, P.O. Box 1177, Sfax 3018, Tunisia.
authors.forename: Imen
authors.initials: I
authors.lastname: Trabelsi
authors.affiliation: Laboratory of Pharmacology, Faculty of Medicine of Sfax, University of Sfax, Avenue Majida Boulila, Sfax 3029, Tunisia.
authors.forename: Sana
authors.initials: S
authors.lastname: Bardaa
authors.affiliation: Laboratory of Microorganisms and Biomolecules (LMB), Centre of Biotechnology of Sfax, Road of Sidi Mansour Km 6, P.O. Box 1177, Sfax 3018, Tunisia.
authors.forename: Mehdi
authors.initials: M
authors.lastname: Triki
authors.affiliation: Laboratory of Enzyme Engineering and Microbiology, National School of Engineering of Sfax, University of Sfax, P.O. 1173-3038, Sfax, Tunisia.
authors.forename: Intidhar
authors.initials: I
authors.lastname: Bkhairia
authors.affiliation: Laboratory of Enzyme Engineering and Microbiology, National School of Engineering of Sfax, University of Sfax, P.O. 1173-3038, Sfax, Tunisia.
authors.forename: Rabab
authors.initials: R
authors.lastname: Ben Slama-Ben Salem
authors.affiliation: Laboratory of Enzyme Engineering and Microbiology, National School of Engineering of Sfax, University of Sfax, P.O. 1173-3038, Sfax, Tunisia.
authors.forename: Moncef
authors.initials: M
authors.lastname: Nasri
authors.affiliation: Laboratory of Microorganisms and Biomolecules (LMB), Centre of Biotechnology of Sfax, Road of Sidi Mansour Km 6, P.O. Box 1177, Sfax 3018, Tunisia. Electronic address: riadh_fss@yahoo.fr.
authors.forename: Riadh
authors.initials: R
authors.lastname: Ben Salah
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology*; D015415:Biomarkers / Q000378:metabolism; D001713:Biphenyl Compounds / Q000737:chemistry; D001760:Bleeding Time; D001835:Body Weight / Q000187:drug effects; D006461:Hemolysis / Q000187:drug effects*; D006861:Hydrogen Peroxide / Q000378:metabolism; D006909:Hydroxyproline / Q000378:metabolism; D007501:Iron / Q000737:chemistry; D008297:Male; D010084:Oxidation-Reduction / Q000187:drug effects; D018384:Oxidative Stress / Q000187:drug effects; D010851:Picrates / Q000737:chemistry; D011134:Polysaccharides / Q000737:chemistry / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D012639:Seeds / Q000737:chemistry*; D012867:Skin / Q000187:drug effects* / Q000378:metabolism; D012879:Skin Physiological Phenomena / Q000187:drug effects; D029910:Trigonella / Q000737:chemistry*; D014945:Wound Healing / Q000187:drug effects*; D019207:beta Carotene / Q000378:metabolism
chemical_list: D000975:Antioxidants; D015415:Biomarkers; D001713:Biphenyl Compounds; D010851:Picrates; D011134:Polysaccharides; D019207:beta Carotene; D006861:Hydrogen Peroxide; C004931:1,1-diphenyl-2-picrylhydrazyl; D007501:Iron; D006909:Hydroxyproline
keywords: Antioxidant activities; Fenugreek seeds; Hemolytic activity; Polysaccharide; Wound healing