title: Enhancement of Cell Adhesion on a Phosphorylcholine-Based Surface through the Interaction with DNA Mediated by Ca2+Ions.
abstract: 2-Methacryloyloxyethyl phosphorylcholine (MPC) has a PC group and is one of the most well-known bioinert polymers. In this study, we evaluated the interaction between MPC and DNA, which specifically interacts with the phospholipid head group via Ca2+ions. A MPC monolayer and poly(MPC) brush were fabricated to observe the effect of the structure on the interaction between MPC and DNA via Ca2+ions. The poly(MPC) brush, which shows higher MPC unit density, more efficiently interacted with DNA via Ca2+ions. Also, serum protein could interact with the poly(MPC) brush via DNA, although the brush itself hardly interacted with serum proteins. Cell adhesion was significantly provoked on poly(MPC)/DNA compared with poly(MPC) because serum protein adsorption was induced on poly(MPC)/DNA.
authors.affiliation: Department of Bioengineering, The University of Tokyo , 7-3-1 Hongo, Bunkyo-ku, 113-8656 Tokyo, Japan.
authors.forename: Tomoyuki
authors.initials: T
authors.lastname: Azuma
authors.affiliation: Department of Bioengineering, The University of Tokyo , 7-3-1 Hongo, Bunkyo-ku, 113-8656 Tokyo, Japan.|Department of Immunology, Genetics and Pathology (IGP), Rudbeck Laboratory C5:3, Uppsala University , SE-751 85 Uppsala, Sweden.
authors.forename: Yuji
authors.initials: Y
authors.lastname: Teramura
authors.affiliation: Department of Materials and Applied Chemistry, College of Science and Technology, Nihon University , 101-8308 Tokyo, Japan.
authors.forename: Toru
authors.initials: T
authors.lastname: Hoshi
authors.affiliation: Department of Bioengineering, The University of Tokyo , 7-3-1 Hongo, Bunkyo-ku, 113-8656 Tokyo, Japan.
authors.forename: Madoka
authors.initials: M
authors.lastname: Takai
mesh_terms: D000818:Animals; D002118:Calcium / Q000737:chemistry*; D002448:Cell Adhesion; D004247:DNA / Q000737:chemistry*; D007477:Ions / Q000737:chemistry; D008297:Male; D015394:Molecular Structure; D010767:Phosphorylcholine / Q000737:chemistry*; D012474:Salmon; D013094:Spermatozoa / Q000737:chemistry* / Q000166:cytology; D013499:Surface Properties
chemical_list: D007477:Ions; D010767:Phosphorylcholine; D004247:DNA; D002118:Calcium