title: [Effect of prolonged propofol infusion on myocardial enzyme, mitochondrial cytochrome C and adenosine  triphosphate in rabbits].
abstract: To explore the effect of long-time propofol infusion on myocardial enzymes, mitochondrial cytochrome C and ATP in rabbits.   Methods: A total of 18 New Zealand rabbits were randomly divided into 3 groups: a control group, a propofol group and an intralipid group. The rabbits were continuously infused with 0.9% normal saline in the control group, 1% propofol in the propofol group, and 10% intralipid in the intralipid group, respectivey. The arterial blood was collected at 0, 8, 16 h and the end of experiment to examine creatine kinase (CK) and creatine kinase isoenzyme (CK-MB). In the end, the myocardial mitochondria from myocardial tissues was separated by differential centrifugation, and mitochondrial cytochrome C content and adenosine triphosphate (ATP) levels were examined by high performance liquid chromatography.  Results: Compared with the control group, the release of cytochrome C from mitochondria were increased in the propofol group and the intralipid group (both P<0.05), but there was no significant difference between them (P>0.05). There was also no significant difference in the ATP content of the mitochondria among the 3 groups (P>0.05). The levels of CK were increased at 8, 16 and 24 h after infusion in the propofol group and the intralipid group compared with that before the infusion (all P<0.05); compared with the control group, the levels of CK were increased at 8, 16 and 24 h after infusion in the propofol group and the intralipid group (all P<0.05); compared with the intralipid group, the levels of CK were increased at 8, 16 and 24 h after infusion in the propofol group (all P>0.05); compared with the control group, the levels of CK-MB were obviously increased in the infusion of propofol for 24 h in the propofol group (P<0.05).  Conclusion: The levels of serum CK increase after the infusion of propofol and intralipid for a long time, and the levels of CK-MB also elevate in the infusion of propofol. Propofol and intralipid can increase the release of myocardial mitochondrial cytochrome C, but they don't affect the ATP production in myocardial mitochondrial.
authors.affiliation: Department of Anesthesia, Sichuan Academy of Medical Sciences/Sichuan Provincial People's Hospital, Chengdu 610072, China.
authors.forename: Guangmin
authors.initials: G
authors.lastname: Xu
authors.affiliation: Department of Anesthesia, Sichuan Academy of Medical Sciences/Sichuan Provincial People's Hospital, Chengdu 610072, China.
authors.forename: Zhixun
authors.initials: Z
authors.lastname: Lan
authors.affiliation: Department of Anesthesia, Sichuan Academy of Medical Sciences/Sichuan Provincial People's Hospital, Chengdu 610072, China.
authors.forename: Xianxiang
authors.initials: X
authors.lastname: Tong
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism*; D000818:Animals; D003402:Creatine Kinase / Q000097:blood / Q000378:metabolism*; D052279:Creatine Kinase, MB Form / Q000097:blood / Q000378:metabolism*; D045304:Cytochromes c / Q000378:metabolism*; D004655:Emulsions / Q000008:administration & dosage / Q000494:pharmacology; D007262:Infusions, Intravenous; D008928:Mitochondria / Q000187:drug effects; D009206:Myocardium / Q000737:chemistry / Q000201:enzymology*; D010743:Phospholipids / Q000008:administration & dosage / Q000494:pharmacology*; D011122:Polyphosphates; D015742:Propofol / Q000008:administration & dosage / Q000494:pharmacology*; D011817:Rabbits; D013024:Soybean Oil / Q000008:administration & dosage / Q000494:pharmacology*
chemical_list: D004655:Emulsions; D010743:Phospholipids; D011122:Polyphosphates; C545823:soybean oil, phospholipid emulsion; D013024:Soybean Oil; D000255:Adenosine Triphosphate; D045304:Cytochromes c; D003402:Creatine Kinase; D052279:Creatine Kinase, MB Form; C005692:triphosphoric acid; D015742:Propofol