title: 3D-printed microfluidic magnetic preconcentrator for the detection of bacterial pathogen using an ATP luminometer and antibody-conjugated magnetic nanoparticles.
abstract: Various types of microfluidic systems have been developed to detect bacterial pathogens. However, most of these require enrichment steps that take at least several hours when detecting bacteria that are present with a low number of cells and, in addition, fabrication requires complicated assembly steps. In this study, we report the development of 3D microfluidic magnetic preconcentrator (3DμFMP) made of plastic via 3D printing without the need for any assembly. 3DμFMP could selectively preconcentrate enterohemorrhagic Escherichia coli O157:H7 in 100mL by a factor of 700 within 1h using antibody-conjugated magnetic nanoparticles (Ab-MNPs). With the combined use of an ATP luminometer, as low as 10 E. coli O157:H7 CFU (colony forming unit)/mL could be detected in blood. These results demonstrate the feasibility of 3DμFMP as a preconcentrator to improve the detection limit of existing bacterial detection systems.
authors.affiliation: Department of Biomedical Engineering, Sungkyunkwan University, Suwon, 16419, Republic of Korea.
authors.forename: Chanyong
authors.initials: C
authors.lastname: Park
authors.affiliation: Department of Biomedical Engineering, Sungkyunkwan University, Suwon, 16419, Republic of Korea.
authors.forename: Jinyeop
authors.initials: J
authors.lastname: Lee
authors.affiliation: School of Mechanical Engineering, Sungkyunkwan University, Suwon, 16419, Republic of Korea.
authors.forename: Yonghee
authors.initials: Y
authors.lastname: Kim
authors.affiliation: School of Mechanical Engineering, Sungkyunkwan University, Suwon, 16419, Republic of Korea.
authors.forename: Jaewon
authors.initials: J
authors.lastname: Kim
authors.affiliation: School of Mechanical Engineering, Sungkyunkwan University, Suwon, 16419, Republic of Korea.
authors.forename: Jinkee
authors.initials: J
authors.lastname: Lee
authors.affiliation: School of Mechanical Engineering, Sungkyunkwan University, Suwon, 16419, Republic of Korea. Electronic address: nanopark@skku.edu.
authors.forename: Sungsu
authors.initials: S
authors.lastname: Park
mesh_terms: D000255:Adenosine Triphosphate / Q000737:chemistry*; D001431:Bacteriological Techniques; D015169:Colony Count, Microbial; D003470:Culture Media; D019453:Escherichia coli O157 / Q000302:isolation & purification*; D005240:Feasibility Studies; D005506:Food Contamination; D005516:Food Microbiology; D005517:Foodborne Diseases / Q000382:microbiology; D057230:Limit of Detection; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D044085:Microfluidics / Q000295:instrumentation*; D008968:Molecular Conformation
chemical_list: D003470:Culture Media; D058185:Magnetite Nanoparticles; D000255:Adenosine Triphosphate
keywords: 3D printing; ATP luminometer; Antibody-conjugated nanoparticles; One-step fabrication; Preconcentration