title: A SUMO-Dependent Protein Network Regulates Chromosome Congression during Oocyte Meiosis.
abstract: During Caenorhabditis elegans oocyte meiosis, a multi-protein ring complex (RC) localized between homologous chromosomes, promotes chromosome congression through the action of the chromokinesin KLP-19. While some RC components are known, the mechanism of RC assembly has remained obscure. We show that SUMO E3 ligase GEI-17/PIAS is required for KLP-19 recruitment to the RC, and proteomic analysis identified KLP-19 as a SUMO substrate in vivo. In vitro analysis revealed that KLP-19 is efficiently sumoylated in a GEI-17-dependent manner, while GEI-17 undergoes extensive auto-sumoylation. GEI-17 and another RC component, the kinase BUB-1, contain functional SUMO interaction motifs (SIMs), allowing them to recruit SUMO modified proteins, including KLP-19, into the RC. Thus, dynamic SUMO modification and the presence of SIMs in RC components generate a SUMO-SIM network that facilitates assembly of the RC. Our results highlight the importance of SUMO-SIM networks in regulating the assembly of dynamic protein complexes.
authors.affiliation: Centre for Gene Regulation and Expression, Sir James Black Centre, School of Life Sciences, University of Dundee, Dundee, DD1 5EH, UK.
authors.forename: Federico
authors.initials: F
authors.lastname: Pelisch
authors.affiliation: Centre for Gene Regulation and Expression, Sir James Black Centre, School of Life Sciences, University of Dundee, Dundee, DD1 5EH, UK.
authors.forename: Triin
authors.initials: T
authors.lastname: Tammsalu
authors.affiliation: Centre for Gene Regulation and Expression, Sir James Black Centre, School of Life Sciences, University of Dundee, Dundee, DD1 5EH, UK.
authors.forename: Bin
authors.initials: B
authors.lastname: Wang
authors.affiliation: Centre for Gene Regulation and Expression, Sir James Black Centre, School of Life Sciences, University of Dundee, Dundee, DD1 5EH, UK.
authors.forename: Ellis G
authors.initials: EG
authors.lastname: Jaffray
authors.affiliation: Centre for Gene Regulation and Expression, Sir James Black Centre, School of Life Sciences, University of Dundee, Dundee, DD1 5EH, UK.
authors.forename: Anton
authors.initials: A
authors.lastname: Gartner
authors.affiliation: Centre for Gene Regulation and Expression, Sir James Black Centre, School of Life Sciences, University of Dundee, Dundee, DD1 5EH, UK. Electronic address: r.t.hay@dundee.ac.uk.
authors.forename: Ronald T
authors.initials: RT
authors.lastname: Hay
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D017173:Caenorhabditis elegans / Q000201:enzymology* / Q000235:genetics; D029742:Caenorhabditis elegans Proteins / Q000235:genetics / Q000378:metabolism*; D045584:Chromosome Positioning; D020090:Chromosome Segregation; D005260:Female; D005838:Genotype; D016547:Kinesins / Q000235:genetics / Q000378:metabolism*; D008025:Ligases / Q000235:genetics / Q000378:metabolism*; D008540:Meiosis; D009865:Oocytes / Q000378:metabolism*; D010641:Phenotype; D011485:Protein Binding; D054730:Protein Interaction Domains and Motifs; D017346:Protein Serine-Threonine Kinases / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction; D058207:Sumoylation; D013997:Time Factors; D044767:Ubiquitin-Protein Ligases / Q000235:genetics / Q000378:metabolism*
chemical_list: D029742:Caenorhabditis elegans Proteins; C493244:KLP-19 protein, C elegans; D044767:Ubiquitin-Protein Ligases; D017346:Protein Serine-Threonine Kinases; C588208:bub-1 protein, C elegans; D016547:Kinesins; C000623110:GEI-17 protein, C elegans; D008025:Ligases
keywords: C. elegans; CRISPR; GEI-17; PIAS; SUMO; SUMO-interaction motif; chromosomes; kinesin; meiosis; spindle