title: Quantification of Type, Timing, and Extent of Cell Body and Nucleus Deformations Caused by the Dimensions and Hydrophilicity of Square Prism Micropillars.
abstract: Novel digital analysis strategies are developed for the quantification of changes in the cytoskeletal and nuclear morphologies of mesenchymal stem cells cultured on micropillars. Severe deformations of nucleus and distinct conformational changes of cell body ranging from extensive elongation to branching are visualized and quantified. These deformations are caused mainly by the dimensions and hydrophilicity of the micropillars.
authors.affiliation: Graduate Department of Biotechnology, Middle East Technical University (METU), Ankara, 06800, Turkey.|BIOMATEN, Center of Excellence in Biomaterials and Tissue Engineering, Middle East Technical University (METU), Ankara, 06800, Turkey.
authors.forename: Onur
authors.initials: O
authors.lastname: Hasturk
authors.affiliation: Institute of Applied Mathematics, Middle East Technical University (METU), Ankara, 06800, Turkey.
authors.forename: Abdullah
authors.initials: A
authors.lastname: Sivas
authors.affiliation: Institute of Applied Mathematics, Middle East Technical University (METU), Ankara, 06800, Turkey.
authors.forename: Bulent
authors.initials: B
authors.lastname: Karasozen
authors.affiliation: Bio-Acoustic-MEMs in Medicine (BAMM) Laboratory, Stanford School of Medicine, Palo Alto, CA, 94394, USA.
authors.forename: Utkan
authors.initials: U
authors.lastname: Demirci
authors.affiliation: Graduate Department of Biotechnology, Middle East Technical University (METU), Ankara, 06800, Turkey.|BIOMATEN, Center of Excellence in Biomaterials and Tissue Engineering, Middle East Technical University (METU), Ankara, 06800, Turkey.|Department of Chemistry, Middle East Technical University (METU), Ankara, 06800, Turkey.
authors.forename: Nesrin
authors.initials: N
authors.lastname: Hasirci
authors.affiliation: Graduate Department of Biotechnology, Middle East Technical University (METU), Ankara, 06800, Turkey.|BIOMATEN, Center of Excellence in Biomaterials and Tissue Engineering, Middle East Technical University (METU), Ankara, 06800, Turkey.|Department of Biological Sciences, Middle East Technical University (METU), Ankara, 06800, Turkey.
authors.forename: Vasif
authors.initials: V
authors.lastname: Hasirci
mesh_terms: D066146:Cell Body / Q000502:physiology*; D002467:Cell Nucleus / Q000502:physiology*; D002478:Cells, Cultured; D003599:Cytoskeleton / Q000502:physiology*; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D059630:Mesenchymal Stem Cells / Q000502:physiology
keywords: conformational changes; cytoskeleton; micropillars; nucleus deformations; oxygen plasma modification