title: Evaluation of hepatoprotective potential of Erythrina indica leaves against antitubercular drugs induced hepatotoxicity in experimental rats.
abstract: BACKGROUND
  Erythrina indica Lam. traditionally used in the treatment of laxative, diuretic, worm infestation, liver ailment and joints pain.
  
  
  OBJECTIVE
  To evaluate the antihepatotoxic potential of Erythrina indica against isoniazid (INH) and rifampicin (RIF) induced hepatotoxicity in rats.
  
  
  METHODS
  Liver toxicity was induced by antitubercular drugs (INH+ RIF) at dose level of 50 mg/kg each, p.o for 28 days. 50% methanolic extract of Erythrina indica (100 and 200 mg/kg) were administered orally once daily for 28 days. The hepatoprotective activity was assessed using various biochemical parameters SGOT, SGPT, ALP, bilirubin, total protein, albumin and LDH. Meanwhile, in vivo antioxidant activities as SOD, CAT, GSH and, LPO were measured in liver homogenate also histological examinations were carried out to assess hepatoprotective activity.
  
  
  METHODS
  The values were subjected to one way analysis of variance (ANOVA) followed by Tukey multiple compare test. Results were considered statistically significant when P < 0.05.
  
  
  RESULTS
  Obtained results demonstrated that the treatment with Erythrina indica (E. indica) significantly prevented drug induced increase in serum levels of hepatic enzymes. Furthermore, Erythrina indica significantly reduced the lipid peroxidation (P < 0.01 tp P < 0.001) in the liver tissue and restored activities of defense antioxidant enzymes GSH (2.15 ± 0.08 to 2.48 ± 0.99; P < 0.05), SOD (2.69 ± 0.752 to 3.712 ± 0.056; P < 0.05 to P < 0.01) and CAT (10.20 ± 0.58 to 12.59 ± 0.42; P < 0.05 to P < 0.001) towards normal. Histopathology of liver tissue showed that Erythrina indica attenuated the hepatocellular necrosis, regeneration and repair of cells toward normal.
  
  
  CONCLUSIONS
  The results of this study strongly indicate the protective effect of Erythrina indica against liver injury which may be attributed to its hepatoprotective activity, and there by scientifically support its traditional use.
authors.affiliation: Department of Pharmacology, Faculty of Pharmacy, Integral University, Lucknow 226026, Uttar Pradesh, India. Electronic address: mujahidiu@gmail.com.
authors.forename: Mohd
authors.initials: M
authors.lastname: Mujahid
authors.affiliation: Department of Pharmacology, Faculty of Pharmacy, Integral University, Lucknow 226026, Uttar Pradesh, India; Department of Pharmacology and Toxicology, College of Pharmacy, University of Hail, P.O. Box 2440, Hail, Saudi Arabia.
authors.forename: Talib
authors.initials: T
authors.lastname: Hussain
authors.affiliation: Department of Pharmacology, Faculty of Pharmacy, Integral University, Lucknow 226026, Uttar Pradesh, India.
authors.forename: Hefazat Hussain
authors.initials: HH
authors.lastname: Siddiqui
authors.affiliation: Department of Pharmacology, Faculty of Pharmacy, Integral University, Lucknow 226026, Uttar Pradesh, India.
authors.forename: Arshad
authors.initials: A
authors.lastname: Hussain
keywords: Anti-oxidant; Anti-tubercular drugs; Erythrina indica; Hepatoprotective