title: gp85 protein vaccine adjuvanted with silica nanoparticles against ALV-J in chickens.
abstract: This study focused on the effect of silica nanoparticles as adjuvant for vaccine applications comprised of gp85, a dominating structural protein of J Subgroup Avian Leukosis Virus (ALV-J), and which was evaluated by comparing with the responsiveness induced by that emulsified in Freund adjuvant. Thirty-six chickens were inoculated twice with gp85 adjuvanted with the silica nanoparticles or Freund's adjuvant at the 2nd and 3rd week old. Two weeks later, the inoculated chickens were challenged with a 102.250% tissue culture infective dose (TCID50) of ALV-J. The blood samples were collected weekly to detect the serum antibodies and viremia. Results showed that positive serum antibodies (S/P value>0.6) against gp85 emerged at the third week in the inoculated chickens, while the antibodies level persisted longer in silica nanoparticles adjuvanted-group to Freund's adjuvanted-group. Furthermore, viremia in silica nanoparticles adjuvanted-group was recovered more quickly compared with Freund's adjuvanted-group. Hence our study revealed that silica nanoparticles can effectively improve the protection of gp85 vaccine against ALV-J and present a better performance than Freund's adjuvant.
authors.affiliation: College of Veterinary Medicine, Shandong Agricultural University, Tai'an 271018, China.
authors.forename: Jia
authors.initials: J
authors.lastname: Cheng
authors.affiliation: College of Veterinary Medicine, Inner Mongolia Agricultural University, Huhhot 011018, China; Dezhou Municipal Finance Bureau, Dezhou 253014, China.
authors.forename: Shiyong
authors.initials: S
authors.lastname: Wen
authors.affiliation: College of Veterinary Medicine, Shandong Agricultural University, Tai'an 271018, China.
authors.forename: Shenghua
authors.initials: S
authors.lastname: Wang
authors.affiliation: College of Veterinary Medicine, Shandong Agricultural University, Tai'an 271018, China.
authors.forename: Pan
authors.initials: P
authors.lastname: Hao
authors.affiliation: College of Veterinary Medicine, Shandong Agricultural University, Tai'an 271018, China.
authors.forename: Ziqiang
authors.initials: Z
authors.lastname: Cheng
authors.affiliation: College of Veterinary Medicine, Shandong Agricultural University, Tai'an 271018, China.
authors.forename: Yongxia
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Research Center for Animal Disease Control Engineering Shandong Province, Shandong Agricultural University, Tai'an 271018, China. Electronic address: zhaopeng@sdau.edu.cn.
authors.forename: Peng
authors.initials: P
authors.lastname: Zhao
authors.affiliation: College of Veterinary Medicine, Shandong Agricultural University, Tai'an 271018, China. Electronic address: Liujz@sdau.edu.cn.
authors.forename: Jianzhu
authors.initials: J
authors.lastname: Liu
mesh_terms: D000276:Adjuvants, Immunologic / Q000008:administration & dosage*; D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D001353:Avian Leukosis / Q000517:prevention & control*; D001354:Avian Leukosis Virus / Q000276:immunology*; D002645:Chickens; D005620:Freund's Adjuvant / Q000008:administration & dosage; D053758:Nanoparticles / Q000008:administration & dosage*; D012822:Silicon Dioxide / Q000008:administration & dosage*; D016896:Treatment Outcome; D014759:Viral Envelope Proteins / Q000276:immunology*; D014765:Viral Vaccines / Q000008:administration & dosage / Q000276:immunology*; D014766:Viremia / Q000517:prevention & control / Q000662:veterinary
chemical_list: D000276:Adjuvants, Immunologic; D000914:Antibodies, Viral; D014759:Viral Envelope Proteins; D014765:Viral Vaccines; C107403:glycoprotein 85, avian leukosis virus; D012822:Silicon Dioxide; D005620:Freund's Adjuvant
keywords: ALV-J; Chicken; Silica nanoparticles; gp85 protein