title: Competitive Argonaute-Based RNA Immunoprecipitation for Investigation of Transcriptomic Response to Anti-miR.
abstract: Identification and validation of microRNA (miRNA) target genes is essential for gaining a better understanding of the many different functions miRNAs have in healthy and diseased cells. From a practical standpoint, validated target genes are also useful for monitoring pharmacological activity of developmental therapeutics that modulate miRNAs, such as anti-miRNA oligonucleotides (anti-miR). Here, we describe a method that uses changes in Argonaute 2-RNA immunoprecipitation in response to competition by anti-miR, titrated ex vivo, as physical evidence for target validation.
authors.affiliation: Regulus Therapeutics, Inc., 3545 John Hopkins Ct., Suite 210, San Diego, CA, 92121-1121, USA. johnandrosavich@gmail.com.
authors.forename: John R
authors.initials: JR
authors.lastname: Androsavich
mesh_terms: D000070416:Antagomirs / Q000235:genetics / Q000627:therapeutic use; D060565:Argonaute Proteins / Q000235:genetics*; D006801:Humans; D047468:Immunoprecipitation / Q000379:methods*; D035683:MicroRNAs / Q000235:genetics* / Q000627:therapeutic use; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D012333:RNA, Messenger / Q000235:genetics; D059467:Transcriptome / Q000235:genetics
chemical_list: D000070416:Antagomirs; D060565:Argonaute Proteins; D035683:MicroRNAs; D012333:RNA, Messenger
keywords: Anti-miR oligonucleotides; Argonaute proteins; Immunoprecipitation; microRNA target validation; microRNAs; microRNAs/antagonists and inhibitors