title: Visualization of RAS/MAPK Signaling In Situ by the Proximity Ligation Assay (PLA).
abstract: RAS/MAPK signaling responds to diverse extracellular cues and regulates a wide array of cellular processes. Given its biological importance, abnormalities in RAS/MAPK signaling cascade have been intimately implicated in numerous human diseases, including cancer. Herein, we describe a novel methodology to study activation of this pivotal signaling pathway. The Proximity Ligation Assay (PLA) is employed to monitor kinase-substrate interactions between MEK1 and HSF1, or MEK1 and ERK1 in situ.
authors.affiliation: The Jackson Laboratory, 600 Main Street, Bar Harbor, ME, 04609, USA.|Graduate Programs, Department of Molecular and Biomedical Sciences, The University of Maine, 5735 Hitchner Hall, Orono, ME, 04469, USA.
authors.forename: Zijian
authors.initials: Z
authors.lastname: Tang
authors.affiliation: The Jackson Laboratory, 600 Main Street, Bar Harbor, ME, 04609, USA. Chengkai.Dai@jax.org.
authors.forename: Chengkai
authors.initials: C
authors.lastname: Dai
mesh_terms: D005455:Fluorescent Antibody Technique; D006367:HeLa Cells; D006801:Humans; D017403:In Situ Hybridization; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism*; D018631:ras Proteins / Q000378:metabolism*
chemical_list: D020928:Mitogen-Activated Protein Kinases; D018631:ras Proteins
keywords: ERK; Fluorescence imaging; HSF1; MAPK signaling; MEK; PLA