title: Rapid Detection of Cat Cystatin C (cCys-C) Using Immuno-Pillar Chips.
abstract: We demonstrated a rapid immunoassay for detection of cat cystatin C (cCys-C) which is an important marker for chronic kidney disease in cats, using immuno-pillar chips. The required amount of reagent solution is 200 times smaller than that for the conventional ELISA in the 96-well microplate (0.5 μL versus 100 μL). In addition, the total assay time in the proposed method is more than 12 times shorter than in the conventional method (20 min versus 240 min). The limit of detection in the new method of 3 ng mL-1is comparable to that of the conventional method (1 ng mL-1) and it is in the clinically relevant range.
authors.affiliation: Graduate School of Chemical Sciences and Engineering, Hokkaido University.
authors.forename: Saeed
authors.initials: S
authors.lastname: Mohammadi
authors.forename: Lori Shayne Alamo
authors.initials: LS
authors.lastname: Busa
authors.forename: Masatoshi
authors.initials: M
authors.lastname: Maeki
authors.forename: Reza M
authors.initials: RM
authors.lastname: Mohamadi
authors.forename: Akihiko
authors.initials: A
authors.lastname: Ishida
authors.forename: Hirofumi
authors.initials: H
authors.lastname: Tani
authors.forename: Manabu
authors.initials: M
authors.lastname: Tokeshi
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000097:blood; D002371:Cat Diseases / Q000097:blood*; D002415:Cats; D055316:Cystatin C / Q000097:blood*; D007118:Immunoassay / Q000379:methods*; D057230:Limit of Detection; D051436:Renal Insufficiency, Chronic / Q000097:blood / Q000662:veterinary*; D013997:Time Factors
chemical_list: D015415:Biomarkers; D055316:Cystatin C