title: Molecular characterization and genotype shift of dengue virus strains between 2001 and 2014 in Guangzhou.
abstract: We studied the evolution, genotypes, and the molecular clock of dengue virus serotype 1 (DENV-1), between 2001 and 2014 in Guangzhou, China. The analysis of the envelope (E) gene sequences of 67 DENV-1 strains isolated in Guangzhou, together with 58 representative sequences downloaded from NCBI, have shown shifts in viral genotypes. The genotype changed several times, from genotype I to IV in 2002, from IV to I in 2005, and from I to V in 2014. These genotype shifts may be the cause of DENV outbreaks. The diversity of genotypes and clades demonstrates a high risk of future outbreaks in Guangzhou. The mean rate of virus nucleotide substitution in Guangzhou was determined to be 7·77 × 10-4 per site per year, which represents a medium substitution rate compared to two other countries. Our research can point to different ancestors of the isolated strains, which may further reveal the different origins and transmission of DENV-1 strains in Guangzhou.
authors.affiliation: Guangzhou Center for Disease Control and Prevention,Guangdong,China.
authors.forename: L Y
authors.initials: LY
authors.lastname: Jiang
authors.affiliation: Guangzhou Center for Disease Control and Prevention,Guangdong,China.
authors.forename: Q L
authors.initials: QL
authors.lastname: Jing
authors.affiliation: Guangzhou Center for Disease Control and Prevention,Guangdong,China.
authors.forename: Y
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Guangzhou Center for Disease Control and Prevention,Guangdong,China.
authors.forename: Y M
authors.initials: YM
authors.lastname: Cao
authors.affiliation: Guangzhou Center for Disease Control and Prevention,Guangdong,China.
authors.forename: W Z
authors.initials: WZ
authors.lastname: Su
authors.affiliation: Guangzhou Center for Disease Control and Prevention,Guangdong,China.
authors.forename: D
authors.initials: D
authors.lastname: Biao
authors.affiliation: Guangzhou Center for Disease Control and Prevention,Guangdong,China.
authors.forename: Z C
authors.initials: ZC
authors.lastname: Yang
mesh_terms: D002681:China / Q000453:epidemiology; D003715:Dengue / Q000453:epidemiology* / Q000821:virology*; D003716:Dengue Virus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D004196:Disease Outbreaks; D019143:Evolution, Molecular; D015686:Gene Products, env / Q000235:genetics; D014644:Genetic Variation; D005838:Genotype; D006801:Humans; D017720:Molecular Epidemiology; D017422:Sequence Analysis, DNA
chemical_list: D015686:Gene Products, env
keywords: Dengue virus; infectious disease; molecular epidemiology; phylogenetic analysis