title: Thermosensitive nanofibers loaded with ciprofloxacin as antibacterial wound dressing materials.
abstract: To obtain wound dressings which could be removed easily without secondary injuries, we prepared thermoresponsive electrospun fiber mats containing poly(di(ethylene glycol) methyl ether methacrylate) (PDEGMA). Blend fibers of PDEGMA and poly(l-lactic acid-co-ε-caprolactone) (P(LLA-CL) were fabricated via electrospinning, and analogous fibers containing the antibiotic ciprofloxacin (CIF) were also prepared. Smooth cylindrical fibers were obtained, albeit with a small amount of beading visible for the ciprofloxacin-loaded fibers. X-ray diffraction showed the drug to exist in the amorphous physical form post-electrospinning. The composite fibers showed distinct thermosensitive properties and gave sustained release of CIF over more than 160h in vitro. The fibers could promote the proliferation of fibroblasts, and by varying the temperature cells could easily be attached to and detached from the fibers. Antibacterial tests demonstrated that fibers loaded with ciprofloxacin were effective in inhibiting the growth of E. coli and S. aureus. In vivo investigations on rats indicated that the composite PDEGMA/P(LLA-CL) fibers loaded with CIF had much more potent wound healing properties than a commercial gauze and CIF-loaded fibers made solely of P(LLA-CL). These results demonstrate the potential of PDEGMA/P(LLA-CL)/ciprofloxacin fibers as advanced wound dressing materials.
authors.affiliation: College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai, 201620, China.
authors.forename: Heyu
authors.initials: H
authors.lastname: Li
authors.affiliation: UCL School of Pharmacy, University College London, 29-39 Brunswick Square, London, WC1N 1AX, UK.
authors.forename: Gareth R
authors.initials: GR
authors.lastname: Williams
authors.affiliation: College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai, 201620, China.
authors.forename: Junzi
authors.initials: J
authors.lastname: Wu
authors.affiliation: College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai, 201620, China.
authors.forename: Yao
authors.initials: Y
authors.lastname: Lv
authors.affiliation: College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai, 201620, China.
authors.forename: Xiaozhu
authors.initials: X
authors.lastname: Sun
authors.affiliation: College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai, 201620, China.
authors.forename: Huanling
authors.initials: H
authors.lastname: Wu
authors.affiliation: College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai, 201620, China. Electronic address: lzhu@dhu.edu.cn.
authors.forename: Li-Min
authors.initials: LM
authors.lastname: Zhu
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000008:administration & dosage* / Q000627:therapeutic use; D001458:Bandages; D002939:Ciprofloxacin / Q000008:administration & dosage* / Q000627:therapeutic use; D004337:Drug Carriers / Q000737:chemistry* / Q000633:toxicity; D065546:Drug Liberation; D004926:Escherichia coli / Q000187:drug effects; D005347:Fibroblasts / Q000166:cytology; D008297:Male; D008689:Methacrylates / Q000737:chemistry / Q000633:toxicity; D008826:Microbial Sensitivity Tests; D057139:Nanofibers / Q000737:chemistry* / Q000633:toxicity; D011091:Polyesters / Q000737:chemistry / Q000633:toxicity; D011092:Polyethylene Glycols / Q000737:chemistry / Q000633:toxicity; D017207:Rats, Sprague-Dawley; D023422:Skin Irritancy Tests; D013211:Staphylococcus aureus / Q000187:drug effects; D023822:Tissue Engineering / Q000379:methods; D014945:Wound Healing / Q000187:drug effects; D014947:Wounds and Injuries / Q000473:pathology / Q000628:therapy*
chemical_list: D000900:Anti-Bacterial Agents; D004337:Drug Carriers; D008689:Methacrylates; D011091:Polyesters; C510557:poly(diethylene glycol monomethacrylate); D011092:Polyethylene Glycols; D002939:Ciprofloxacin; C062968:lactide-caprolactone copolymer
keywords: Ciprofloxacin; Electrospinning; P(LLA-CL); PDEGMA; Thermosensitive; Wound dressing