title: Down syndrome critical region 1 positively correlates with angiogenesis in hypopharyngeal cancer.
abstract: Hypopharyngeal carcinoma has one of the highest mortality rates of head and neck cancer, therefore, the identification of markers associated with the pathogenesis and development of hypopharyngeal cancer is critical. Down syndrome critical region 1 (DSCR1) is associated with carcinogenesis and tumor growth in several types of malignancy. Activation of the vascular endothelial growth factor (VEGF) signaling pathway upregulates DSCR1. The aims of the present study were to determine the expression levels of DSCR1 and VEGF‑C in hypopharyngeal cancer, and investigate the association between DSCR1 and angiogenesis in the disease. Tissue samples from 94 cases of pathologically confirmed hypopharyngeal squamous cell carcinoma were collected. The mRNA levels of DSCR1 and VEGF‑C in cancerous and paracancerous tissues were examined using semi‑quantitative reverse transcription‑polymerase chain reaction. Microvessel density (MVD) was counted, according to the number of cluster of differentiation 34‑positive cells. Spearman's correlation analysis was utilized to analyze the association between DSCR1 and angiogenesis. The relative mRNA expression levels of DSCR1 and VEGF‑C, and the MVD were significantly increased in the cancerous tissue samples from the patients with hypopharyngeal cancer, compared with the paracancerous tissue samples from these patients. Higher levels of DSCR1 and increased MVD were associated with poorly differentiated tumors and lymph node metastasis. The mRNA expression levels of DSCR1 were positively correlated with the mRNA levels of VEGF‑C in the cancerous tissues. The protein expression levels of DSCR1 were also positively correlated with MVD in the cancerous tissues. The results indicated that DSCR1 is involved in tumor angiogenesis in patients with hypopharyngeal cancer, and is closely associated with the progression of the disease.
authors.affiliation: Department of Otorhinolaryngology, Yantaishan Hospital, Yantai, Shandong 264000, P.R. China.
authors.forename: Chao
authors.initials: C
authors.lastname: Lv
authors.affiliation: Department of Otorhinolaryngology, Qilu Hospital, Shandong University, Jinan, Shandong 250012, P.R. China.
authors.forename: Dayu
authors.initials: D
authors.lastname: Liu
authors.affiliation: Department of Otorhinolaryngology, Yantaishan Hospital, Yantai, Shandong 264000, P.R. China.
authors.forename: Xiaona
authors.initials: X
authors.lastname: Wei
mesh_terms: D004268:DNA-Binding Proteins; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007012:Hypopharyngeal Neoplasms / Q000098:blood supply / Q000235:genetics* / Q000473:pathology; D007013:Hypopharynx / Q000098:blood supply / Q000378:metabolism / Q000473:pathology; D047908:Intracellular Signaling Peptides and Proteins / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D009124:Muscle Proteins / Q000235:genetics*; D009389:Neovascularization, Pathologic / Q000235:genetics* / Q000473:pathology; D012333:RNA, Messenger / Q000235:genetics; D015854:Up-Regulation; D042582:Vascular Endothelial Growth Factor C / Q000235:genetics*
chemical_list: D004268:DNA-Binding Proteins; D047908:Intracellular Signaling Peptides and Proteins; D009124:Muscle Proteins; C108612:RCAN1 protein, human; D012333:RNA, Messenger; C467486:VEGFC protein, human; D042582:Vascular Endothelial Growth Factor C