title: Molecular cloning, expression and functional analysis of three subunits of protein phosphatase 2A (PP2A) from black tiger shrimps (Penaeus monodon).
abstract: Protein phosphatase 2A (PP2A) is a cellular serine-threonine (Ser/Thr) phosphatase that plays a crucial role in regulating most cellular functions. In the present study, the full-length cDNAs of three subunits of PmPP2A (PmPP2A-A, PP2A-B and PP2A-C) were cloned from Penaeus monodon, which are the first available for shrimps. Sequence analysis showed that PmPP2A-A, PmPP2A-B and PmPP2A-C encoded polypeptides of 591, 443, and 324 amino acids, respectively. The mRNAs of three subunits of PmPP2A were expressed constitutively in all tissues examined, and predominantly in the ovaries. In ovarian maturation stages, the three subunits of PmPP2A were continuously but differentially expressed. Dopamine and 5-hydroxytryptamine injection experiments were conducted to study the expression profile of three subunits of PmPP2A, and the results indicated that PmPP2A played a negative regulatory role in the process of ovarian maturation. In addition, the recombinant proteins of three subunits of PmPP2A were successfully obtained, and the phosphatase activity of PmPP2A was tested in vitro. The results of this study will advance our understanding about the molecular mechanisms of PmPP2A in Penaeus monodon.
authors.affiliation: South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, China; Key Laboratory of South China Sea Fishery Resources Exploitation & Utilization, Ministry of Agriculture, Guangzhou 510300, China.
authors.forename: Chao
authors.initials: C
authors.lastname: Zhao
authors.affiliation: South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, China; College of Aqua-life Science and Technology, Shanghai Ocean University, Shanghai 201306, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Wang
authors.affiliation: South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, China; Key Laboratory of South China Sea Fishery Resources Exploitation & Utilization, Ministry of Agriculture, Guangzhou 510300, China.
authors.forename: Mingjun
authors.initials: M
authors.lastname: Fu
authors.affiliation: South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, China.
authors.forename: Keng
authors.initials: K
authors.lastname: Yang
authors.affiliation: South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, China; Tropical Aquaculture Research and Development Center of South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Sanya 572018, China. Electronic address: qiugroup_bio@outlook.com.
authors.forename: Lihua
authors.initials: L
authors.lastname: Qiu
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004298:Dopamine / Q000494:pharmacology; D005260:Female; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D009865:Oocytes / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism; D010053:Ovary / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism; D033561:Penaeidae / Q000201:enzymology* / Q000235:genetics* / Q000254:growth & development; D054648:Protein Phosphatase 2 / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D021122:Protein Subunits / Q000235:genetics* / Q000378:metabolism*; D012701:Serotonin / Q000494:pharmacology
chemical_list: D021122:Protein Subunits; D012701:Serotonin; D054648:Protein Phosphatase 2; D004298:Dopamine
keywords: 5-Hydroxytryptamine and dopamine; Penaeus monodon; Phosphatase activity; Protein phosphatase 2A; mRNA expression