title: Erythrocyte survival is controlled by microRNA-142.
abstract: Hematopoietic-specific microRNA-142 is a critical regulator of various blood cell lineages, but its role in erythrocytes is unexplored. Herein, we characterize the impact of microRNA-142 on erythrocyte physiology and molecular cell biology, using a mouse loss-of-function allele. We report that microRNA-142 is required for maintaining the typical erythrocyte biconcave shape and structural resilience, for the normal metabolism of reactive oxygen species, and for overall lifespan. microRNA-142 further controls ACTIN filament homeostasis and membrane skeleton organization. The analyses presented reveal previously unappreciated functions of microRNA-142 and contribute to an emerging view of small RNAs as key players in erythropoiesis. Finally, the work herein demonstrates how a housekeeping network of cytoskeletal regulators can be reshaped by a single micro-RNA denominator in a cell type specific manner.
authors.affiliation: Department of Molecular Genetics, Weizmann Institute of Science, Rehovot, Israel.
authors.forename: Natalia
authors.initials: N
authors.lastname: Rivkin
authors.affiliation: Department of Molecular Genetics, Weizmann Institute of Science, Rehovot, Israel.
authors.forename: Elik
authors.initials: E
authors.lastname: Chapnik
authors.affiliation: Department of Immunology Weizmann Institute of Science, Rehovot, Israel.
authors.forename: Alexander
authors.initials: A
authors.lastname: Mildner
authors.affiliation: Department of Biochemistry and Molecular Biology, Hebrew university, Hadassah Medical School, Jerusalem, Israel.
authors.forename: Gregory
authors.initials: G
authors.lastname: Barshtein
authors.affiliation: Flow Cytometry Unit, Life Sciences Core Facilities, Weizmann Institute of Science, Rehovot, Israel.
authors.forename: Ziv
authors.initials: Z
authors.lastname: Porat
authors.affiliation: Department of Chemical Research Support, Weizmann Institute of Science, Rehovot, Israel.
authors.forename: Elena
authors.initials: E
authors.lastname: Kartvelishvily
authors.affiliation: Department of Chemical Research Support, Weizmann Institute of Science, Rehovot, Israel.
authors.forename: Tali
authors.initials: T
authors.lastname: Dadosh
authors.affiliation: Functional Genomics and Leukemic Research, Cancer Research Center, Sheba Medical Center, Ramat Gan, Israel.
authors.forename: Yehudit
authors.initials: Y
authors.lastname: Birger
authors.affiliation: Department of Pathology, Hadassah Medical Center, Jerusalem, Israel.
authors.forename: Gail
authors.initials: G
authors.lastname: Amir
authors.affiliation: Department of Biochemistry and Molecular Biology, Hebrew university, Hadassah Medical School, Jerusalem, Israel.
authors.forename: Saul
authors.initials: S
authors.lastname: Yedgar
authors.affiliation: Functional Genomics and Leukemic Research, Cancer Research Center, Sheba Medical Center, Ramat Gan, Israel.|Department of Human Molecular Genetics and Biochemistry, Tel Aviv University, Jerusalem, Israel.
authors.forename: Shai
authors.initials: S
authors.lastname: Izraeli
authors.affiliation: Department of Immunology Weizmann Institute of Science, Rehovot, Israel.
authors.forename: Steffen
authors.initials: S
authors.lastname: Jung
authors.affiliation: Department of Molecular Genetics, Weizmann Institute of Science, Rehovot, Israel eran.hornstein@weizmann.ac.il.
authors.forename: Eran
authors.initials: E
authors.lastname: Hornstein
mesh_terms: D000818:Animals; D002460:Cell Line; D002470:Cell Survival / Q000235:genetics*; D004905:Erythrocyte Aging / Q000235:genetics*; D004912:Erythrocytes / Q000378:metabolism* / Q000473:pathology / Q000648:ultrastructure; D004920:Erythropoiesis / Q000235:genetics; D006801:Humans; D051379:Mice; D018345:Mice, Knockout; D035683:MicroRNAs / Q000235:genetics*; D010084:Oxidation-Reduction; D017382:Reactive Oxygen Species
chemical_list: D035683:MicroRNAs; C529102:Mirn142 microRNA, mouse; D017382:Reactive Oxygen Species