title: Targeted Apoptosis of Parietal Cells Is Insufficient to Induce Metaplasia in Stomach.
abstract: Parietal cell atrophy is considered to cause metaplasia in the stomach. We developed mice that express the diphtheria toxin receptor specifically in parietal cells to induce their death, and found this to increase proliferation in the normal stem cell zone and neck but not to cause metaplastic reprogramming of chief cells. Furthermore, the metaplasia-inducing agents tamoxifen or DMP-777 still induced metaplasia even after previous destruction of parietal cells by diphtheria toxin. Atrophy of parietal cells alone therefore is not sufficient to induce metaplasia: completion of metaplastic reprogramming of chief cells requires mechanisms beyond parietal cell injury or death.
authors.affiliation: Division of Gastroenterology, Department of Medicine, Department of Pathology and Immunology, Department of Developmental Biology, Washington University, St. Louis, Missouri.
authors.forename: Joseph
authors.initials: J
authors.lastname: Burclaff
authors.affiliation: Division of Gastroenterology, Department of Medicine, Department of Pathology and Immunology, Department of Developmental Biology, Washington University, St. Louis, Missouri.
authors.forename: Luciana H
authors.initials: LH
authors.lastname: Osaki
authors.affiliation: Institute of Combined Injury, College of Preventive Medicine, State Key Laboratory of Trauma, Burns and Combined Injury, Third Military Medical University, Chongqing, China.
authors.forename: Dengqun
authors.initials: D
authors.lastname: Liu
authors.affiliation: Nashville Veterans Affairs Medical Center, Epithelial Biology Center, Department of Surgery, and Department of Cell and Developmental Biology, Vanderbilt University School of Medicine, Nashville, Tennessee.
authors.forename: James R
authors.initials: JR
authors.lastname: Goldenring
authors.affiliation: Division of Gastroenterology, Department of Medicine, Department of Pathology and Immunology, Department of Developmental Biology, Washington University, St. Louis, Missouri. Electronic address: jmills@wustl.edu.
authors.forename: Jason C
authors.initials: JC
authors.lastname: Mills
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects / Q000235:genetics; D001284:Atrophy / Q000139:chemically induced; D001384:Azetidines; D049109:Cell Proliferation; D065150:Cellular Reprogramming; D019872:Chief Cells, Gastric / Q000378:metabolism / Q000473:pathology*; D004167:Diphtheria Toxin / Q000494:pharmacology; D066257:Heparin-binding EGF-like Growth Factor / Q000235:genetics; D036341:Intercellular Signaling Peptides and Proteins; D007437:Intrinsic Factor / Q000378:metabolism; D008679:Metaplasia / Q000139:chemically induced / Q000235:genetics / Q000378:metabolism; D051379:Mice; D010295:Parietal Cells, Gastric / Q000187:drug effects / Q000473:pathology* / Q000502:physiology*; D010455:Peptides / Q000378:metabolism; D010879:Piperazines; D037121:Plant Lectins / Q000378:metabolism; D013270:Stomach / Q000473:pathology*; D013629:Tamoxifen
chemical_list: D001384:Azetidines; D004167:Diphtheria Toxin; C045475:Griffonia simplicifolia lectins; D066257:Heparin-binding EGF-like Growth Factor; D036341:Intercellular Signaling Peptides and Proteins; D010455:Peptides; D010879:Piperazines; D037121:Plant Lectins; C451060:spasmolytic polypeptide; D013629:Tamoxifen; C105999:DMP 777; D007437:Intrinsic Factor
keywords: Atp4b-Cre; CD44 Variant 9; Organoids; SPEM