title: Epidermal Growth Factor Rescues Endothelial Dysfunction in Primary Human Tissues In Vitro.
abstract: Preeclampsia is a hypertensive disorder of pregnancy, responsible for over 60 000 maternal deaths annually. Endothelial dysfunction is a central aspect to its pathophysiology, and currently, no medical therapeutic is available for its treatment. In this study, we aim to investigate the effect of epidermal growth factor (EGF) on endothelial dysfunction using primary human tissues. We performed a number of in vitro assays that mimic the vascular endothelial dysfunction that occurs in preeclampsia. Epidermal growth factor reduced the expression of vascular cell adhesion molecule-1, a marker of endothelial dysfunction, after insult with tumor necrosis factor α (TNF-α) or serum from women with preeclampsia. Additionally, after TNF-α insult, EGF reduced tube disruption and the adhesion of monocytes to primary human umbilical vein endothelial cells (HUVECs). Our findings suggest that EGF reduces endothelial dysfunction in primary HUVECs. Epidermal growth factor may have potential as a novel peptide treatment for preeclampsia and other diseases where there is endothelial dysfunction.
authors.affiliation: 1 Department of Obstetrics and Gynaecology, Translational Obstetrics Group, Mercy Hospital for Women, University of Melbourne, Melbourne, Victoria, Australia.
authors.forename: Roxanne
authors.initials: R
authors.lastname: Hastie
authors.affiliation: 1 Department of Obstetrics and Gynaecology, Translational Obstetrics Group, Mercy Hospital for Women, University of Melbourne, Melbourne, Victoria, Australia.
authors.forename: Stephen
authors.initials: S
authors.lastname: Tong
authors.affiliation: 1 Department of Obstetrics and Gynaecology, Translational Obstetrics Group, Mercy Hospital for Women, University of Melbourne, Melbourne, Victoria, Australia.
authors.forename: Natalie J
authors.initials: NJ
authors.lastname: Hannan
authors.affiliation: 1 Department of Obstetrics and Gynaecology, Translational Obstetrics Group, Mercy Hospital for Women, University of Melbourne, Melbourne, Victoria, Australia.
authors.forename: Fiona
authors.initials: F
authors.lastname: Brownfoot
authors.affiliation: 1 Department of Obstetrics and Gynaecology, Translational Obstetrics Group, Mercy Hospital for Women, University of Melbourne, Melbourne, Victoria, Australia.
authors.forename: Ping
authors.initials: P
authors.lastname: Cannon
authors.affiliation: 1 Department of Obstetrics and Gynaecology, Translational Obstetrics Group, Mercy Hospital for Women, University of Melbourne, Melbourne, Victoria, Australia.
authors.forename: Tu'uhevaha J
authors.initials: TJ
authors.lastname: Kaitu'u-Lino
mesh_terms: D004730:Endothelium, Vascular / Q000187:drug effects* / Q000378:metabolism; D004815:Epidermal Growth Factor / Q000494:pharmacology*; D005260:Female; D061307:Human Umbilical Vein Endothelial Cells / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D009000:Monocytes / Q000187:drug effects / Q000378:metabolism; D011225:Pre-Eclampsia / Q000378:metabolism; D011247:Pregnancy; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology; D019010:Vascular Cell Adhesion Molecule-1 / Q000378:metabolism*
chemical_list: D014409:Tumor Necrosis Factor-alpha; D019010:Vascular Cell Adhesion Molecule-1; D004815:Epidermal Growth Factor
keywords: endothelial dysfunction; epidermal growth factor; preeclampsia