title: Interactions between circulating nanoengineered polymer particles and extracellular matrix components in vitro.
abstract: The extracellular matrix (ECM) that surrounds cells in vivo represents a biological barrier for nanomaterials in biomedicine. Herein, we present a system for investigating the interactions between circulating polymer particles and ECM components in vitro using a commercially available flow-based device. We use this system to show how material-dependent interactions of two different particle types-one assembled using poly(ethylene glycol) (PEG) and one prepared using poly(methacrylic acid) (PMA)-affect their interactions with basement membrane extracts during in vitro circulation, with PEG particles remaining in circulation longer than PMA particles. Further, by comparing macroporous hyaluronic acid gel constructs (typically used for tissue engineering) with basement membrane extracts, we show that scaffold-effects (porosity and surface chemistry) impact on circulation time in vitro. The presented system is simple and modular, and can be used to rapidly screen fundamental interactions of engineered particles with biologically relevant microenvironments under flow-conditions.
authors.affiliation: ARC Centre of Excellence in Convergent Bio-Nano Science and Technology, and the Department of Chemical and Biomolecular Engineering, The University of Melbourne, Parkville, Victoria 3010, Australia. fcaruso@unimelb.edu.au.
authors.forename: Julia A
authors.initials: JA
authors.lastname: Braunger
authors.affiliation: ARC Centre of Excellence in Convergent Bio-Nano Science and Technology, and the Department of Chemical and Biomolecular Engineering, The University of Melbourne, Parkville, Victoria 3010, Australia. fcaruso@unimelb.edu.au.
authors.forename: Mattias
authors.identifier: http://orcid.org/0000-0002-9876-7079
authors.initials: M
authors.lastname: Björnmalm
authors.affiliation: ARC Centre of Excellence in Convergent Bio-Nano Science and Technology, and the Department of Chemical and Biomolecular Engineering, The University of Melbourne, Parkville, Victoria 3010, Australia. fcaruso@unimelb.edu.au.
authors.forename: Nathan A
authors.initials: NA
authors.lastname: Isles
authors.affiliation: ARC Centre of Excellence in Convergent Bio-Nano Science and Technology, and the Department of Chemical and Biomolecular Engineering, The University of Melbourne, Parkville, Victoria 3010, Australia. fcaruso@unimelb.edu.au.
authors.forename: Jiwei
authors.initials: J
authors.lastname: Cui
authors.affiliation: Department of Chemical and Biomolecular Engineering, The University of Melbourne, Parkville, Victoria 3010, Australia.
authors.forename: Timothy M A
authors.initials: TM
authors.lastname: Henderson
authors.affiliation: Department of Chemical and Biomolecular Engineering, The University of Melbourne, Parkville, Victoria 3010, Australia.
authors.forename: Andrea J
authors.identifier: http://orcid.org/0000-0001-6925-885X
authors.initials: AJ
authors.lastname: O'Connor
authors.affiliation: ARC Centre of Excellence in Convergent Bio-Nano Science and Technology, and the Department of Chemical and Biomolecular Engineering, The University of Melbourne, Parkville, Victoria 3010, Australia. fcaruso@unimelb.edu.au.
authors.forename: Frank
authors.identifier: http://orcid.org/0000-0002-0197-497X
authors.initials: F
authors.lastname: Caruso
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D005109:Extracellular Matrix / Q000737:chemistry*; D005434:Flow Cytometry; D006801:Humans; D051379:Mice; D018613:Microscopy, Confocal; D053758:Nanoparticles / Q000737:chemistry*; D036103:Nanotechnology; D010316:Particle Size; D011092:Polyethylene Glycols / Q000737:chemistry*; D011109:Polymethacrylic Acids / Q000737:chemistry*; D016062:Porosity; D013499:Surface Properties
chemical_list: D011109:Polymethacrylic Acids; C030613:polymethacrylic acid; D011092:Polyethylene Glycols