title: Anti-anaphylactic effects ofTrichilia monadelpha(Thonn.) J. J. De Wilde extracts on rodent models of anaphylaxis.
abstract: Effects of petroleum ether and ethanolic extracts ofTrichilia monadelphastem bark (PEE and EAE) on compound 48/80-induced systemic and passive anaphylaxis were determined. Survival rate, extravasation, degranulation of mast cells, and secretion of tumour necrosis factor-α (TNF-α) and interleukin-6 (IL-6) were measured after pre-treatment with extracts (10-100 mg/kg) and disodium chromoglycate (2.5-250 μg/kg) and induction of anaphylaxis in C57BL/6 mice or Sprague-Dawley rats with compound 48/80. Histopathological assessments were made from skin biopsies of rats. Data was analyzed by Kaplan-Meier Survival Log-Rank Analysis, or One-way ANOVA and Holm-Sidak's post hoc test. PEE and EAE inhibited (P ≤ 0.0001) tremors in systemic anaphylaxis passive cutaneous anaphylactic reactions and extravasation, stabilized or prevented (P ≤ 0.001-0.0001) mast cell degranulation, and inhibited (P ≤ 0.001-0.0001) TNF-α and IL-6 secretion. Per the findings, PEE and EAE ofT. monadelphahave exhibited substantial anti-anaphylactic and anti-inflammatory property (with PEE performing better) which substantiates its use traditionally in management of allergies and other inflammatory disorders.
authors.affiliation: Department of Pharmacology and Toxicology, Faculty of Pharmacy, Madonna University, Elele Campus, Rivers State, Nigeria.
authors.forename: Inemesit Okon
authors.initials: IO
authors.lastname: Ben
authors.affiliation: Department of Pharmacology, Faculty of Pharmacy and Pharmaceutical Sciences, College of Health Sciences, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana.
authors.forename: Eric
authors.initials: E
authors.lastname: Woode
authors.affiliation: Department of Pharmacology, Faculty of Pharmacy and Pharmaceutical Sciences, College of Health Sciences, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana.
authors.forename: George Asumeng
authors.initials: GA
authors.lastname: Koffuor
authors.affiliation: Department of Pathology, School of Medical Sciences / Komfo Anokye Teaching Hospital, College of Health Sciences, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana.
authors.forename: Emmanuel Akomanin
authors.initials: EA
authors.lastname: Asiamah
keywords: Anaphylaxis; Interleukin-6; Mast cells degranulation; TNF-α; Trichilia monadelpha