title: Quantitative Fluorescence In Situ Hybridization (QFISH).
abstract: Fluorescence in situ hybridization (FISH) has a wide spectrum of applications in current molecular cytogenetic and cancer research. This is a unique technique that can be used for chromosomal DNA analysis in all cell types, at all stages of the cell cycle, and at molecular resolution. Recent developments in microscopy and imaging systems have allowed quantification of digital FISH images (quantitative FISH or QFISH) and have provided a new way for molecular cytogenetic analysis at single-cell level. QFISH can be applied for studying chromosome imbalances in interphase nuclei or metaphase spreads, measuring relative DNA content at chromosomal loci and identifying parental origin of homologous chromosomes. Here, a QFISH protocol suitable for the majority of DNA probes using the popular US National Institute of Health developed ImageJ software is described.
authors.affiliation: Mental Health Research Center, Zagorodnoe sh. 2, Moscow, 117152, Russia. ivan.iourov@gmail.com.|Separated Structural Unit "Clinical Research Institute of Pediatrics" named after Y.E. Veltishev, Russian National Research Medical University named after N.I. Pirogov, Ministry of Health of Russian Federation, Moscow, Russia. ivan.iourov@gmail.com.|Moscow State University of Psychology and Education, Moscow, Russia. ivan.iourov@gmail.com.
authors.forename: Ivan Y
authors.initials: IY
authors.lastname: Iourov
mesh_terms: D002869:Chromosome Aberrations; D015342:DNA Probes; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D007399:Interphase / Q000235:genetics; D008856:Microscopy, Fluorescence / Q000379:methods
chemical_list: D015342:DNA Probes
keywords: Chromosome abnormalities; DNA probes; Fluorescence in situ hybridization; Interphase; Nucleus; QFISH; Quantification