title: Developing a rat model of dilated cardiomyopathy with improved survival.
abstract: To compare the continuous infusion and intermittent bolus injection administration protocols of doxorubicin (Dox) under the same cumulative dose (12 mg/kg), and establish a rat dilated cardiomyopathy model with improved survival, a total of 150 Sprague-Dawley (SD) rats were divided into three groups: a control group, administered with normal saline; a Dox 1 group, administration twice a week at 1 mg/kg; a Dox 2, administration once a week at 2 mg/kg. Mortality rates in the Dox 1 and Dox 2 groups were 22% and 48%, respectively (P<0.05). As shown by echocardiography, both Dox groups exhibited significant chamber dilatation and reduced cardiac function (all P<0.05 vs. control). Plasma brain natriuretic peptide and C-reactive protein concentrations were significantly increased (P<0.05) with both Dox regimens. The concentrations of Caspase-3 in myocardial tissues of rats significantly increased in both doxorubicin regimens. Myocardial metabolism imaging by histology and18F-fluoro-deoxyglucose-positron emission tomography (18FDG-PET) both revealed decreased myocardial viability and necrosis, and even interstitial fibrosis, in left ventricles (LVs) in both Dox groups. Serum creatinine and aspartate aminotransferase concentrations were significantly higher in the Dox 2 model than in the Dox 1 model. Doxorubicin given at both regimens induced dilated cardiomyopathy, while its administration at lower doses with more frequent infusions reduced the mortality rate.
authors.affiliation: Wuxi Hospital of Traditional Chinese Medicine, Wuxi Hospital Affiliated to Nanjing University of Chinese Medicine, Wuxi 214071, China.
authors.forename: Li-Juan
authors.initials: LJ
authors.lastname: Shen
authors.affiliation: Wuxi Hospital of Traditional Chinese Medicine, Wuxi Hospital Affiliated to Nanjing University of Chinese Medicine, Wuxi 214071, China.
authors.forename: Shu
authors.initials: S
authors.lastname: Lu
authors.affiliation: Jiangsu Institute of Parasitic Diseases, Key Laboratory on Technology for Parasitic Disease Prevention and Control, Ministry of Health, Jiangsu Provincial Key Laboratory on Molecular Biology of Parasites, Jiangsu Provincial Key Subject on Parasitic Diseases, Wuxi 214064, China.
authors.forename: Yong-Hua
authors.initials: YH
authors.lastname: Zhou
authors.affiliation: Department of Ultrasonography, Wuxi Hospital of Traditional Chinese Medicine, Wuxi Hospital Affiliated to Nanjing University of Chinese Medicine, Wuxi 214071, China.
authors.forename: Lan
authors.initials: L
authors.lastname: Li
authors.affiliation: Wuxi Hospital of Traditional Chinese Medicine, Wuxi Hospital Affiliated to Nanjing University of Chinese Medicine, Wuxi 214071, China.
authors.forename: Qing-Min
authors.initials: QM
authors.lastname: Xing
authors.affiliation: Jiangsu Institute of Parasitic Diseases, Key Laboratory on Technology for Parasitic Disease Prevention and Control, Ministry of Health, Jiangsu Provincial Key Laboratory on Molecular Biology of Parasites, Jiangsu Provincial Key Subject on Parasitic Diseases, Wuxi 214064, China.
authors.forename: Yong-Liang
authors.initials: YL
authors.lastname: Xu
mesh_terms: D000818:Animals; D002097:C-Reactive Protein / Q000032:analysis; D002311:Cardiomyopathy, Dilated / Q000139:chemically induced* / Q000378:metabolism / Q000473:pathology; D053148:Caspase 3 / Q000235:genetics; D004195:Disease Models, Animal; D004317:Doxorubicin / Q000633:toxicity*; D008297:Male; D009206:Myocardium / Q000378:metabolism / Q000473:pathology; D020097:Natriuretic Peptide, Brain / Q000097:blood; D000072078:Positron Emission Tomography Computed Tomography; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D020097:Natriuretic Peptide, Brain; D004317:Doxorubicin; D002097:C-Reactive Protein; D053148:Caspase 3
keywords: 
  					18FDG-PET; Animal model; Dilated cardiomyopathy; Doxorubicin