title: Incomplete and delayed Sox2 deletion defines residual ear neurosensory development and maintenance.
abstract: The role of Sox2 in neurosensory development is not yet fully understood. Using mice with conditional Islet1-cre mediated deletion of Sox2, we explored the function of Sox2 in neurosensory development in a model with limited cell type diversification, the inner ear. In Sox2 conditional mutants, neurons initially appear to form normally, whereas late- differentiating neurons of the cochlear apex never form. Variable numbers of hair cells differentiate in the utricle, saccule, and cochlear base but sensory epithelium formation is completely absent in the apex and all three cristae of the semicircular canal ampullae. Hair cells differentiate only in sensory epithelia known or proposed to have a lineage relationship of neurons and hair cells. All initially formed neurons lacking hair cell targets die by apoptosis days after they project toward non-existing epithelia. Therefore, late neuronal development depends directly on Sox2 for differentiation and on the survival of hair cells, possibly derived from common neurosensory precursors.
authors.affiliation: Institute of Biotechnology CAS, Prague, Czechia.|Faculty of Science, Charles University, Prague, Czechia.
authors.forename: Martina
authors.initials: M
authors.lastname: Dvorakova
authors.affiliation: Department of Biology, University of Iowa, Iowa City, IA, USA.
authors.forename: Israt
authors.initials: I
authors.lastname: Jahan
authors.affiliation: Institute of Biotechnology CAS, Prague, Czechia.|Faculty of Science, Charles University, Prague, Czechia.
authors.forename: Iva
authors.initials: I
authors.lastname: Macova
authors.affiliation: Institute of Experimental Medicine CAS, Prague, Czechia.
authors.forename: Tetyana
authors.initials: T
authors.lastname: Chumak
authors.affiliation: Institute of Biotechnology CAS, Prague, Czechia.
authors.forename: Romana
authors.initials: R
authors.lastname: Bohuslavova
authors.affiliation: Institute of Experimental Medicine CAS, Prague, Czechia.
authors.forename: Josef
authors.initials: J
authors.lastname: Syka
authors.affiliation: Department of Biology, University of Iowa, Iowa City, IA, USA.
authors.forename: Bernd
authors.initials: B
authors.lastname: Fritzsch
authors.affiliation: Institute of Biotechnology CAS, Prague, Czechia.
authors.forename: Gabriela
authors.initials: G
authors.lastname: Pavlinkova
mesh_terms: D000818:Animals; D017353:Gene Deletion; D006198:Hair Cells, Auditory / Q000166:cytology / Q000378:metabolism*; D051379:Mice; D008822:Mice, Transgenic; D055495:Neurogenesis / Q000502:physiology*; D055748:SOXB1 Transcription Factors / Q000235:genetics / Q000378:metabolism*; D012444:Saccule and Utricle / Q000166:cytology / Q000196:embryology*
chemical_list: D055748:SOXB1 Transcription Factors; C527783:Sox2 protein, mouse