title: Determination of Anti-miR Association with miRNA/Argonaute Complexes In Vivo.
abstract: Aberrant expression of microRNAs (miRNAs) has been causatively linked to multiple disease pathologies while pharmacological inhibition of overexpressed miRNAs by modified oligonucleotides, termed anti-miRs, has been shown to ameliorate the disease phenotype. Anti-miRs are also widely used in academia to define miRNA-mediated regulation of gene networks in vitro and in vivo. Here, we describe a methodology that allows the determination of the physical association of miRNA inhibitors and their targets in the context of the Argonaute complex in vivo, providing unprecedented insight into the physiological interactions of anti-miRs and the miRNA machinery.
authors.affiliation: Regulus Therapeutics, 3545 John Hopkins Ct., Suite 210, San Diego, CA, 92121-1121, USA. dzisoulis@regulusrx.com.
authors.forename: Dimitrios G
authors.initials: DG
authors.lastname: Zisoulis
mesh_terms: D060565:Argonaute Proteins / Q000737:chemistry / Q000235:genetics*; D055808:Drug Discovery; D005786:Gene Expression Regulation; D053263:Gene Regulatory Networks / Q000235:genetics; D006801:Humans; D035683:MicroRNAs / Q000037:antagonists & inhibitors / Q000235:genetics* / Q000627:therapeutic use; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D009841:Oligonucleotides / Q000235:genetics* / Q000627:therapeutic use
chemical_list: D060565:Argonaute Proteins; D035683:MicroRNAs; D009841:Oligonucleotides
keywords: Anti-miR; Argonaute; Drug discovery; Oligonucleotide therapeutics; microRNA; microRNA inhibitors