title: Effect of hyperthyroidism on circulating prolactin and hypothalamic expression of tyrosine hydroxylase, prolactin signaling cascade members and estrogen and progesterone receptors during late pregnancy and lactation in the rat.
abstract: Hyperthyroidism (HyperT) compromises pregnancy and lactation, hindering suckling-induced PRL release. We studied the effect of HyperT on hypothalamic mRNA (RT-qPCR) and protein (Western blot) expression of tyrosine hydroxylase (TH), PRL receptor (PRLR) and signaling pathway members, estrogen-α (ERα) and progesterone (PR) receptors on late pregnancy (days G19, 20 and 21) and early lactation (L2) in rats. HyperT advanced pre-partum PRL release, reduced circulating PRL on L2 and increased TH mRNA (G21 and L2), p-TH, PRLR mRNA, STAT5 protein (G19 and L2), PRLR protein (G21) and CIS protein (G19). PRs mRNAs and protein decreased on G19 but afterwards PRA mRNA (G20), PRB mRNA (G21) and PRA mRNA and protein (L2) increased. ERα protein increased on G19 and decreased on G20. Thus, the altered hypothalamic PRLR, STAT5, PR and ERα expression in hyperthyroid rats may induce elevated TH expression and activation, that consequently, elevate dopaminergic tone during lactation, blunting suckling-induced PRL release and litter growth.
authors.affiliation: Laboratorio de Reproducción y Lactancia, Instituto de Medicina y Biología Experimental de Cuyo (IMBECU), CCT-CONICET Mendoza, Argentina; Facultad de Ciencias Exactas y Naturales, Universidad Nacional de Cuyo, Mendoza, Argentina. Electronic address: gpennacchio@mendoza-conicet.gov.ar.
authors.forename: Gisela E
authors.initials: GE
authors.lastname: Pennacchio
authors.affiliation: Laboratorio de Reproducción y Lactancia, Instituto de Medicina y Biología Experimental de Cuyo (IMBECU), CCT-CONICET Mendoza, Argentina.
authors.forename: Flavia J
authors.initials: FJ
authors.lastname: Neira
authors.affiliation: Laboratorio de Reproducción y Lactancia, Instituto de Medicina y Biología Experimental de Cuyo (IMBECU), CCT-CONICET Mendoza, Argentina; Instituto de Fisiología, Facultad de Ciencias Médicas, Universidad Nacional de Cuyo, Mendoza, Argentina.
authors.forename: Marta
authors.initials: M
authors.lastname: Soaje
authors.affiliation: Laboratorio de Reproducción y Lactancia, Instituto de Medicina y Biología Experimental de Cuyo (IMBECU), CCT-CONICET Mendoza, Argentina.
authors.forename: Graciela A
authors.initials: GA
authors.lastname: Jahn
authors.affiliation: Laboratorio de Reproducción y Lactancia, Instituto de Medicina y Biología Experimental de Cuyo (IMBECU), CCT-CONICET Mendoza, Argentina; Facultad de Ciencias Exactas y Naturales, Universidad Nacional de Cuyo, Mendoza, Argentina. Electronic address: svaldez@mendoza-conicet.gov.ar.
authors.forename: Susana R
authors.initials: SR
authors.lastname: Valdez
mesh_terms: D000818:Animals; D001942:Breast Feeding / Q000379:methods; D004298:Dopamine / Q000378:metabolism; D004967:Estrogens / Q000378:metabolism; D005260:Female; D006980:Hyperthyroidism / Q000473:pathology*; D007031:Hypothalamus / Q000378:metabolism*; D007774:Lactation / Q000378:metabolism*; D008321:Mammary Glands, Animal / Q000378:metabolism; D011247:Pregnancy; D011248:Pregnancy Complications / Q000378:metabolism; D011270:Pregnancy, Animal / Q000378:metabolism; D011374:Progesterone / Q000378:metabolism; D011388:Prolactin / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D011960:Receptors, Estrogen / Q000378:metabolism*; D011980:Receptors, Progesterone / Q000378:metabolism*; D011981:Receptors, Prolactin / Q000378:metabolism; D015398:Signal Transduction / Q000502:physiology; D014446:Tyrosine 3-Monooxygenase / Q000378:metabolism*
chemical_list: D004967:Estrogens; D012333:RNA, Messenger; D011960:Receptors, Estrogen; D011980:Receptors, Progesterone; D011981:Receptors, Prolactin; D011374:Progesterone; D011388:Prolactin; D014446:Tyrosine 3-Monooxygenase; D004298:Dopamine
keywords: Hyperthyroidism; Hypothalamic dopaminergic neurons; Lactation; PRL; PRL signaling pathway; Pregnancy; Tyrosine hydroxylase