title: Glut1 promotes cell proliferation, migration and invasion by regulating epidermal growth factor receptor and integrin signaling in triple-negative breast cancer cells.
abstract: Elevated glucose levels in cancer cells can be attributed to increased levels of glucose transporter (GLUT) proteins. Glut1 expression is increased in human malignant cells. To investigate alternative roles of Glut1 in breast cancer, we silenced Glut1 in triple-negative breast-cancer cell lines using a short hairpin RNA (shRNA) system. Glut1 silencing was verified by Western blotting and qRT-PCR. Knockdown of Glut1 resulted in decreased cell proliferation, glucose uptake, migration, and invasion through modulation of the EGFR/ MAPK signaling pathway and integrin β1/Src/FAK signaling pathways. These results suggest that Glut1 not only plays a role as a glucose transporter, but also acts as a regulator of signaling cascades in the tumorigenesis of breast cancer. [BMB Reports 2017; 50(3): 132-137].
authors.affiliation: Department of Life Science, Hanyang University, Seoul 04763, Korea.
authors.forename: Sunhwa
authors.initials: S
authors.lastname: Oh
authors.affiliation: Department of Life Science, Hanyang University, Seoul 04763, Korea.
authors.forename: Hyungjoo
authors.initials: H
authors.lastname: Kim
authors.affiliation: Department of Life Science, Hanyang University, Seoul 04763, Korea.
authors.forename: KeeSoo
authors.initials: K
authors.lastname: Nam
authors.affiliation: Department of Life Science and Natural Science Institute, Hanyang University, Seoul 04763, Korea.
authors.forename: Incheol
authors.initials: I
authors.lastname: Shin
mesh_terms: D017209:Apoptosis; D001943:Breast Neoplasms / Q000235:genetics; D018929:Cell Culture Techniques; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000502:physiology; D049109:Cell Proliferation / Q000502:physiology; D002471:Cell Transformation, Neoplastic; D066246:ErbB Receptors / Q000235:genetics / Q000378:metabolism*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D055785:Gene Knockdown Techniques; D051272:Glucose Transporter Type 1 / Q000378:metabolism* / Q000502:physiology; D006801:Humans; D016023:Integrins / Q000378:metabolism; D034741:RNA, Small Interfering / Q000235:genetics; D015398:Signal Transduction / Q000502:physiology; D064726:Triple Negative Breast Neoplasms / Q000378:metabolism*
chemical_list: D051272:Glucose Transporter Type 1; D016023:Integrins; D034741:RNA, Small Interfering; C495677:SLC2A1 protein, human; C512478:EGFR protein, human; D066246:ErbB Receptors