title: Enzymatically Modified Starch Favorably Modulated Intestinal Transit Time and Hindgut Fermentation in Growing Pigs.
abstract: Aside from being used as stabilizing agents in many processed foods, chemically modified starches may act as functional dietary ingredients. Therefore, development of chemically modified starches that are less digestible in the upper intestinal segments and promote fermentation in the hindgut receives considerable attention. This study aimed to investigate the impact of an enzymatically modified starch (EMS) on nutrient flow, passage rate, and bacterial activity at ileal and post-ileal level. Eight ileal-cannulated growing pigs were fed 2 diets containing 72% purified starch (EMS or waxy cornstarch as control) in a cross-over design for 10 d, followed by a 4-d collection of feces and 2-d collection of ileal digesta. On d 17, solid and liquid phase markers were added to the diet to determine ileal digesta flow for 8 h after feeding. Reduced small intestinal digestion after the consumption of the EMS diet was indicated by a 10%-increase in ileal flow and fecal excretion of dry matter and energy compared to the control diet (P<0.05). Moreover, EMS feeding reduced ileal transit time of both liquid and solid fractions compared to the control diet (P<0.05). The greater substrate flow to the large intestine with the EMS diet increased the concentrations of total and individual short-chain fatty acids (SCFA) in feces (P<0.05). Total bacterial 16S rRNA gene abundance was not affected by diet, whereas the relative abundance of the Lactobacillus group decreased (P<0.01) by 50% and of Enterobacteriaceae tended (P<0.1) to increase by 20% in ileal digesta with the EMS diet compared to the control diet. In conclusion, EMS appears to resemble a slowly digestible starch by reducing intestinal transit and increasing SCFA in the distal large intestine.
authors.affiliation: Institute of Animal Nutrition and Functional Plant Compounds, University of Veterinary Medicine Vienna, Vienna, Austria.|Research Cluster "Animal Gut Health", Department for Farm Animals and Veterinary Public Health, University of Veterinary Medicine Vienna, Vienna, Austria.
authors.forename: M A
authors.initials: MA
authors.lastname: Newman
authors.affiliation: Institute of Animal Nutrition and Functional Plant Compounds, University of Veterinary Medicine Vienna, Vienna, Austria.|Research Cluster "Animal Gut Health", Department for Farm Animals and Veterinary Public Health, University of Veterinary Medicine Vienna, Vienna, Austria.
authors.forename: Q
authors.initials: Q
authors.lastname: Zebeli
authors.affiliation: Equine University Clinic, Department for Companion Animals and Horses, University of Veterinary Medicine, Vienna, Austria.
authors.forename: K
authors.initials: K
authors.lastname: Velde
authors.affiliation: Agrana Research & Innovation Center GmbH, Tulln, Austria.
authors.forename: D
authors.initials: D
authors.lastname: Grüll
authors.affiliation: Agrana Research & Innovation Center GmbH, Tulln, Austria.
authors.forename: T
authors.initials: T
authors.lastname: Molnar
authors.affiliation: Center for Analytical Chemistry, Department of Agrobiotechnology, IFA-Tulln, University of Natural Resources and Life Sciences, Vienna, Austria.
authors.forename: W
authors.initials: W
authors.lastname: Kandler
authors.affiliation: Research Cluster "Animal Gut Health", Department for Farm Animals and Veterinary Public Health, University of Veterinary Medicine Vienna, Vienna, Austria.|University Clinic for Swine, University of Veterinary Medicine, Vienna, Austria.
authors.forename: B U
authors.initials: BU
authors.lastname: Metzler-Zebeli
mesh_terms: D000821:Animal Feed / Q000032:analysis; D000824:Animal Nutritional Physiological Phenomena; D000818:Animals; D004063:Digestion; D005285:Fermentation; D055951:Functional Food / Q000032:analysis; D005772:Gastrointestinal Transit; D007082:Ileum / Q000382:microbiology / Q000502:physiology; D007422:Intestines / Q000382:microbiology / Q000502:physiology*; D008297:Male; D013213:Starch / Q000031:analogs & derivatives* / Q000378:metabolism*; D013552:Swine / Q000254:growth & development / Q000502:physiology*
chemical_list: D013213:Starch