title: Synthesis of a fluorescent photoaffinity probe of OSW-1 by site-selective acylation of an inactive congener and biological evaluation.
abstract: A novel fluorescent photoaffinity probe of OSW-1 was prepared in two steps from a naturally occurring inactive congener by a sequential site-selective acylation strategy using Me2SnCl2. It displayed highly potent anticancer activity and a similar intracellular localization property to that of a fluorescently-tagged OSW-1, thereby demonstrating its potential utility in live cell studies.
authors.affiliation: Tokyo University of Agriculture and Technology, Department of Biotechnology and Life Science, Koganei-shi, Tokyo 184-8588, Japan. sakuraik@cc.tuat.ac.jp.
authors.forename: K
authors.initials: K
authors.lastname: Sakurai
authors.affiliation: Tokyo University of Agriculture and Technology, Department of Biotechnology and Life Science, Koganei-shi, Tokyo 184-8588, Japan. sakuraik@cc.tuat.ac.jp.
authors.forename: M
authors.initials: M
authors.lastname: Hiraizumi
authors.affiliation: Tokyo University of Agriculture and Technology, Department of Biotechnology and Life Science, Koganei-shi, Tokyo 184-8588, Japan. sakuraik@cc.tuat.ac.jp.
authors.forename: N
authors.initials: N
authors.lastname: Isogai
authors.affiliation: Tokyo University of Agriculture and Technology, Department of Biotechnology and Life Science, Koganei-shi, Tokyo 184-8588, Japan. sakuraik@cc.tuat.ac.jp.
authors.forename: R
authors.initials: R
authors.lastname: Komatsu
authors.affiliation: Tokyo University of Agriculture and Technology, Department of Biotechnology and Life Science, Koganei-shi, Tokyo 184-8588, Japan. sakuraik@cc.tuat.ac.jp.
authors.forename: T
authors.initials: T
authors.lastname: Shibata
authors.affiliation: Tokyo University of Agriculture and Technology, Department of Biotechnology and Life Science, Koganei-shi, Tokyo 184-8588, Japan. sakuraik@cc.tuat.ac.jp.
authors.forename: Y
authors.initials: Y
authors.lastname: Ohta
mesh_terms: D000215:Acylation; D000970:Antineoplastic Agents / Q000138:chemical synthesis* / Q000493:pharmacokinetics / Q000494:pharmacology; D049109:Cell Proliferation / Q000187:drug effects; D002783:Cholestenones / Q000138:chemical synthesis* / Q000493:pharmacokinetics / Q000494:pharmacology; D005456:Fluorescent Dyes / Q000138:chemical synthesis* / Q000493:pharmacokinetics / Q000494:pharmacology; D006367:HeLa Cells; D006801:Humans; D009369:Neoplasms / Q000188:drug therapy; D019920:Photoaffinity Labels / Q000138:chemical synthesis* / Q000493:pharmacokinetics / Q000494:pharmacology; D012503:Saponins / Q000138:chemical synthesis* / Q000493:pharmacokinetics / Q000494:pharmacology
chemical_list: D000970:Antineoplastic Agents; D002783:Cholestenones; D005456:Fluorescent Dyes; D019920:Photoaffinity Labels; D012503:Saponins; C106408:OSW 1