title: Composition of endophytic fungal community associated with leaves of maize cultivated in south Brazilian field.
abstract: The objective of this study was to conduct a survey about fungi associated with leaves from two different maize plant lineages and to analyze their microbiota diversity. Isolated fungi were identified by morphological analysis and molecular taxonomy was performed using ITS1-5.8S-ITS2 rDNA. About 27 fungi morphotypes were obtained, 15 of them were from the first maize lineage. About 86.7% of the individuals belonged to the Dothideomycetes class (Phoma sorghina, Epicocum nigrum, Cladosporium sp., Bipolaris zeicola, and Alternaria alternata complex) and 13.3% to the Sordariomycetes class (Diaporthe/Phomopsis sp. and Nigrospora sp.). This ratio was opposite in the other maize lineage with 25.0% of Dothideomycetes (E. nigrum and Pleosporales) and 75.0% of Sordariomycetes (Gibberella fujikuroi complex, Fusarium graminearum complex, Diaporthe/Phomopsis sp., and Nigrospora sp.). By concerning the analyses of morphological characteristics and molecular phylogeny, this study intended to identify the groups of saprophytic, phytopathogenic, and mycotoxin fungi, which differently co-inhabit leaf tissue of maize plants in both tested lineages.
authors.affiliation: 1 Department of Plant Science and Crop Protection, Federal University of Paraná , Curitiba, Brazil.
authors.forename: Vivian J
authors.initials: VJ
authors.lastname: Szilagyi-Zecchin
authors.affiliation: 2 Department of Genetics, Federal University of Paraná , Curitiba, Brazil.
authors.forename: Douglas
authors.initials: D
authors.lastname: Adamoski
authors.affiliation: 3 Department of Basic Pathology, Federal University of Paraná , Curitiba, Brazil.
authors.forename: Renata Rodrigues
authors.initials: RR
authors.lastname: Gomes
authors.affiliation: 4 Soil Biotechnology Laboratory, Embrapa Soja , Londrina, Brazil.
authors.forename: Mariangela
authors.initials: M
authors.lastname: Hungria
authors.affiliation: 2 Department of Genetics, Federal University of Paraná , Curitiba, Brazil.
authors.forename: Angela C
authors.initials: AC
authors.lastname: Ikeda
authors.affiliation: 2 Department of Genetics, Federal University of Paraná , Curitiba, Brazil.
authors.forename: Vanessa
authors.initials: V
authors.lastname: Kava-Cordeiro
authors.affiliation: 2 Department of Genetics, Federal University of Paraná , Curitiba, Brazil.
authors.forename: Chirlei
authors.initials: C
authors.lastname: Glienke
authors.affiliation: 2 Department of Genetics, Federal University of Paraná , Curitiba, Brazil.
authors.forename: Lygia V
authors.initials: LV
authors.lastname: Galli-Terasawa
mesh_terms: D044822:Biodiversity; D001938:Brazil; D060026:Endophytes / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D005658:Fungi / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D010802:Phylogeny; D018515:Plant Leaves / Q000382:microbiology*; D003313:Zea mays / Q000254:growth & development / Q000382:microbiology*
keywords: ITS1-5.8S-ITS2; fungi; microbiota; molecular phylogeny