title: Epidemiological and pathological investigation of fowl aviadenovirus serotypes 8b and 11 isolated from chickens with inclusion body hepatitis in Spain (2011-2013).
abstract: Inclusion body hepatitis caused by different fowl aviadenovirus (FAdV) serotypes has been described in several countries in recent years. In Spain, from the spring of 2011 to 2013, an increased number of outbreaks in broiler and broiler breeder flocks from different regions occurred. The objectives of the present work were to carry out the molecular characterization of FAdV strains from Spanish inclusion body hepatitis cases and to study the pathogenicity and viral dynamics of these strains in specific pathogen-free (SPF) chickens. A total of 52 inclusion body hepatitis clinical cases, including 45 from broiler farms and seven from broiler breeder farms, were analysed by conventional polymerase chain reaction and sequencing targeting the FAdV hexon gene. From these, 37 strains were classified as FAdV type 8b, while the remaining 15 were classified as FAdV types 11 (n = 10), 2 (n = 4) and 8a (n = 1). In addition, two different FAdVs belonging to the genotypes 8b and 11 were used for experimental infection. Specific pathogen-free five-day-old birds were inoculated intramuscularly with a high (106.5tissue culture infective dose (TCID)50/ml) or low (104TCID50/ml) dose of the above-mentioned FAdVs. No mortality was observed in any of the experimental groups, and only one bird showed evident clinical signs. However, macroscopic and microscopic hepatic lesions, as well as viral DNA, were detected in birds from all infection groups. Inclusion bodies and viral DNA were also detected in the pancreas and in the small and the large intestine in some birds. Long-lasting shedding and transmission to contact birds were confirmed in all infected groups.
authors.affiliation: a IRTA, Centre de Recerca en Sanitat Animal (CReSA, IRTA-UAB) , Campus de la Universitat Autònoma de Barcelona , Bellaterra , Spain.
authors.forename: S
authors.initials: S
authors.lastname: Oliver-Ferrando
authors.affiliation: a IRTA, Centre de Recerca en Sanitat Animal (CReSA, IRTA-UAB) , Campus de la Universitat Autònoma de Barcelona , Bellaterra , Spain.
authors.forename: R
authors.initials: R
authors.lastname: Dolz
authors.affiliation: b Universidade Estadual do Norte do Paraná - UENP/CLM, Centro de Ciências Agrárias , Bandeirantes , Brazil.
authors.forename: C
authors.initials: C
authors.lastname: Calderón
authors.affiliation: a IRTA, Centre de Recerca en Sanitat Animal (CReSA, IRTA-UAB) , Campus de la Universitat Autònoma de Barcelona , Bellaterra , Spain.
authors.forename: R
authors.initials: R
authors.lastname: Valle
authors.affiliation: a IRTA, Centre de Recerca en Sanitat Animal (CReSA, IRTA-UAB) , Campus de la Universitat Autònoma de Barcelona , Bellaterra , Spain.
authors.forename: R
authors.initials: R
authors.lastname: Rivas
authors.affiliation: a IRTA, Centre de Recerca en Sanitat Animal (CReSA, IRTA-UAB) , Campus de la Universitat Autònoma de Barcelona , Bellaterra , Spain.
authors.forename: M
authors.initials: M
authors.lastname: Pérez
authors.affiliation: c Centre de Sanitat Avícola de Catalunya (CESAC) , Tarragona , Spain.
authors.forename: M
authors.initials: M
authors.lastname: Biarnés
authors.affiliation: c Centre de Sanitat Avícola de Catalunya (CESAC) , Tarragona , Spain.
authors.forename: A
authors.initials: A
authors.lastname: Blanco
authors.affiliation: a IRTA, Centre de Recerca en Sanitat Animal (CReSA, IRTA-UAB) , Campus de la Universitat Autònoma de Barcelona , Bellaterra , Spain.
authors.forename: K
authors.initials: K
authors.lastname: Bertran
authors.affiliation: d UAB, Centre de Recerca en Sanitat Animal (CReSA, IRTA-UAB) , Campus de la Universitat Autònoma de Barcelona , Bellaterra , Spain.|e Departament de Sanitat i Anatomia Animals , Facultat de Veterinària , Bellaterra , Spain.
authors.forename: A
authors.initials: A
authors.lastname: Ramis
authors.affiliation: a IRTA, Centre de Recerca en Sanitat Animal (CReSA, IRTA-UAB) , Campus de la Universitat Autònoma de Barcelona , Bellaterra , Spain.
authors.forename: N
authors.initials: N
authors.lastname: Busquets
authors.affiliation: d UAB, Centre de Recerca en Sanitat Animal (CReSA, IRTA-UAB) , Campus de la Universitat Autònoma de Barcelona , Bellaterra , Spain.|e Departament de Sanitat i Anatomia Animals , Facultat de Veterinària , Bellaterra , Spain.
authors.forename: N
authors.initials: N
authors.lastname: Majó
mesh_terms: D000257:Adenoviridae Infections / Q000453:epidemiology / Q000473:pathology / Q000662:veterinary* / Q000821:virology; D000818:Animals; D000259:Aviadenovirus / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification / Q000472:pathogenicity; D002645:Chickens / Q000821:virology*; D004196:Disease Outbreaks / Q000662:veterinary*; D060005:Genotyping Techniques; D006524:Hepatitis, Viral, Animal / Q000453:epidemiology* / Q000473:pathology / Q000821:virology; D007181:Inclusion Bodies, Viral; D008099:Liver / Q000473:pathology / Q000821:virology; D010802:Phylogeny; D011201:Poultry Diseases / Q000453:epidemiology* / Q000473:pathology / Q000821:virology; D017422:Sequence Analysis, DNA / Q000662:veterinary; D065288:Serogroup; D013030:Spain / Q000453:epidemiology; D013047:Specific Pathogen-Free Organisms; D017201:Virus Shedding
keywords: Fowl aviadenovirus (FAdV); chicken; inclusion body hepatitis (IBH); serotype 11; serotype 8b