title: Protein biochip-based semiquantitative detection for plasma leptin.
abstract: Plasma leptin is secreted from adipose tissues and plays pivotal roles in human physiological and pathological processes. Here, we aimed at conducting a protein biochip-based sandwich-like approach for detection of plasma leptin among healthy individuals, obesity, and diabetes patients.
  
  
  
  Totally, 96 plasma samples, including 45 healthy individuals with standard body mass index (BMI), 28 obesity and 23 diabetes patients, were recruited in the study. Plasma leptin was detected by a well-established protein biochip. Meanwhile an ELISA was also performed for assessment of the leptin detection by the protein biochip.
  
  
  
  We found that the plasma leptin level in the obesity and diabetes patients was significantly higher than that in healthy individuals with standard body mass index (p < 0.001). The limit detection concentration of leptin was as low as 0.006 μg/mL. The plasma leptin could be semiquantitatively detected by the protein biochip. The compatibility of the biochip-based detection approach seemed acceptable in comparison with the ELISA assay (R2= 0.948).
  
  
  
  We provided a protein biochip-based approach for plasma detection. This approach would be a potential substitution for the ELISA assay.
authors.affiliation: Department of Pathology, the First Affiliated Hospital, Anhui Medical University, Hefei, P.R. China.
authors.forename: Lei
authors.initials: L
authors.lastname: Ye
authors.affiliation: Department of Medicine, Immunology Institute, Icahn School of Medicine at Mount Sinai, New York, NY, USA.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Hu
authors.affiliation: Department of Medicine, Immunology Institute, Icahn School of Medicine at Mount Sinai, New York, NY, USA.
authors.forename: Fei-Hong
authors.initials: FH
authors.lastname: Xu
authors.affiliation: Anhui Academic Institute of Biology, Hefei, Anhui, P.R. China.
authors.forename: Cheng-Yun
authors.initials: CY
authors.lastname: Cai
authors.affiliation: Anhui Academic Institute of Biology, Hefei, Anhui, P.R. China.
authors.forename: Da-Wei
authors.initials: DW
authors.lastname: Song
authors.affiliation: Anhui Academic Institute of Biology, Hefei, Anhui, P.R. China.
authors.forename: Zhen-Shan
authors.initials: ZS
authors.lastname: Xu
authors.affiliation: Department of Pathology, the First Affiliated Hospital, Anhui Medical University, Hefei, P.R. China.
authors.forename: Wei-Dong
authors.initials: WD
authors.lastname: Du
mesh_terms: D000328:Adult; D000911:Antibodies, Monoclonal / Q000276:immunology; D001774:Blood Chemical Analysis / Q000379:methods*; D005260:Female; D006801:Humans; D020738:Leptin / Q000097:blood* / Q000276:immunology; D057230:Limit of Detection; D008297:Male; D008875:Middle Aged; D040081:Protein Array Analysis; D055815:Young Adult
chemical_list: D000911:Antibodies, Monoclonal; D020738:Leptin
keywords: Diabetes; Enzyme-linked immunosorbent assay; Leptin; Obesity; Protein biochip