title: Screening and Preliminary Verification of a Phage Display Single-Chain Antibody Library Against Coal Workers' Pneumoconiosis.
abstract: To construct a phage display human antibody library (PDHAL) against pneumoconiosis for the diagnosis and treatment of coal worker pneumoconiosis (CWP).
  
  
  
  The PDHAL was established via CWP blood and six positive antibodies were discovered. 867 coal workers (558 CWP and 309 without CWP) and 393 controls were recruited to validate the results.
  
  
  
  A PDHAL against CWP was established, from which six strong positive clones were selected, sequenced and identified as VEGF, interleukin-18, HSP70, HER3, Gz-B and RF. Logistic regression analysis revealed that VEGF (OR (95% CI), 0.02 (0.01to 0.07), P < 0.05), RF-Ab (OR (95% CI): 0.46 (0.28 to 0.73), P < 0.05) and HSP70/HSP-70-Ab (OR (95% CI): 0.71 (0.53 to 0.95), P < 0.05) were protective factors for CWP after adjustment of confounding factors.
  
  
  
  The serum VEGF, RF-Ab and HSP-70/HSP-70 antibodies were potential biomarkers for diagnosis and treatment of CWP.
authors.affiliation: School of Public Health (Dr Zhang, Dr Hao, Mr Zhang, Dr Yao), and First Affiliated Hospital of Zhengzhou University (Mr Li), Zhengzhou University; Henan Collaborative Innovation Center of Molecular Diagnosis and Laboratory Medicine, Key Laboratory of Environmental Pollution and Health Effects, School of Public Health, Xinxiang Medical University, Xinxiang (Drs Zhang, Ren, Gao, Ding, Wu); and Henan Provincial Institute of Occupational Health (Dr Jiao), Zhengzhou, China.
authors.forename: Guang-Hui
authors.initials: GH
authors.lastname: Zhang
authors.forename: Li
authors.initials: L
authors.lastname: Li
authors.forename: Changfu
authors.initials: C
authors.lastname: Hao
authors.forename: Jing-Chao
authors.initials: JC
authors.lastname: Ren
authors.forename: Hongyi
authors.initials: H
authors.lastname: Zhang
authors.forename: Jie
authors.initials: J
authors.lastname: Jiao
authors.forename: Liyun
authors.initials: L
authors.lastname: Gao
authors.forename: Shibin
authors.initials: S
authors.lastname: Ding
authors.forename: Sanqiao
authors.initials: S
authors.lastname: Yao
authors.forename: Wu
authors.initials: W
authors.lastname: Yao
authors.forename: Weidong
authors.initials: W
authors.lastname: Wu
mesh_terms: D000328:Adult; D055008:Anthracosis / Q000175:diagnosis* / Q000628:therapy*; D015415:Biomarkers / Q000097:blood; D003031:Coal; D003032:Coal Mining; D006801:Humans; D008297:Male; D008875:Middle Aged; D019151:Peptide Library; D057127:Single-Chain Antibodies / Q000737:chemistry* / Q000627:therapeutic use
chemical_list: D015415:Biomarkers; D003031:Coal; D019151:Peptide Library; D057127:Single-Chain Antibodies