title: Quantitative optical coherence microscopy for the in situ investigation of the biofilm.
abstract: This paper explores the potential of optical coherence microscopy (OCM) for the <italic<in situ</italic< monitoring of biofilm growth. The quantitative imaging of the early developmental biology of a representative biofilm, <italic<Klebsiella pneumonia</italic< (KP-1), was performed using a swept source-based Fourier domain OCM system. The growth dynamics of the KP-1 biofilms and their transient response under perturbation was investigated using the enface visualization of microcolonies and their spatial localization. Furthermore, the optical density (OD) and planar density of the biofilms are calculated using an OCM technique and compared with OD and colony forming units measured using standard procedures via the sampling of the flow-cell effluent.
authors.affiliation: Nanyang Technological University, Centre for Optical and Laser Engineering, School of Mechanical and Aerospace Engineering, 50 Nanyang Avenue, Singapore 639798, Singapore.
authors.forename: Ratheesh Kumar
authors.initials: RK
authors.lastname: Meleppat
authors.affiliation: Nanyang Technological University, Biological Process Laboratory, School of Mechanical and Aerospace Engineering, 50 Nanyang Avenue, Singapore 639798, Singapore.
authors.forename: Christopher
authors.initials: C
authors.lastname: Shearwood
authors.affiliation: Nanyang Technological University, Centre for Optical and Laser Engineering, School of Mechanical and Aerospace Engineering, 50 Nanyang Avenue, Singapore 639798, Singapore.
authors.forename: Seah Leong
authors.initials: SL
authors.lastname: Keey
authors.affiliation: Nanyang Technological University, Centre for Optical and Laser Engineering, School of Mechanical and Aerospace Engineering, 50 Nanyang Avenue, Singapore 639798, Singapore.
authors.forename: Murukeshan Vadakke
authors.initials: MV
authors.lastname: Matham
mesh_terms: D018441:Biofilms; D004867:Equipment Design; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D007711:Klebsiella pneumoniae; D008853:Microscopy / Q000379:methods*; D041623:Tomography, Optical Coherence / Q000379:methods*