title: cPKCγ membrane translocation is involved in herkinorin‑induced neuroprotection against cerebral ischemia/reperfusion injury in mice.
abstract: Herkinorin is an opiate analgesic with limited adverse effects, functioning as a primary selective atypical opioid µ agonist. The present study aimed to identify whether herkinorin has a positive effect on ischemic/reperfusion (I/R) injury. Adult male C57BL/6 mice were randomly divided into five groups: i) Naïve, ii) sham, iii) I/R, iv) I/R with dimethyl sulfoxide (I/R+D) and v) I/R with herkinorin (I/R+H). The I/R injury model was induced by occluding the middle cerebral artery for 1 h followed by 24 h or 7 days of reperfusion. Neurobehavioral scores and sensorimotor functions were examined 24 h and 7 days following reperfusion. In addition, infarct volumes were examined at these time points using a 2,3,5‑triphenyltetrazolium chloride assay. Herkinorin treatment improved neurobehavioral and sensorimotor functional recovery from I/R‑induced brain injury. There was a significant decrease in infarct volume in the I/R+H group at 24 h or 7 days following reperfusion compared with the I/R and I/R+D groups. Western blotting suggested that the decrease in conventional protein kinase C γ (cPKCγ) membrane translocation in the peri‑infarct region may be attenuated by herkinorin pretreatment. These results indicated that herkinorin may be beneficial in I/R‑induced mouse brain injury, and this may be attributed to the membrane translocation of cPKCγ following activation.
authors.affiliation: Department of Anesthesiology, Beijing Tongren Hospital, Capital Medical University, Beijing 100730, P.R. China.
authors.forename: Xiaochen
authors.initials: X
authors.lastname: Gui
authors.affiliation: Department of Anesthesiology, Beijing Tongren Hospital, Capital Medical University, Beijing 100730, P.R. China.
authors.forename: Xu
authors.initials: X
authors.lastname: Cui
authors.affiliation: Department of Neurobiology, Beijing Institute for Brain Disorders, Capital Medical University, Beijing 100069, P.R. China.
authors.forename: Haiping
authors.initials: H
authors.lastname: Wei
authors.affiliation: Department of Anesthesiology, Beijing Tongren Hospital, Capital Medical University, Beijing 100730, P.R. China.
authors.forename: Guang
authors.initials: G
authors.lastname: Feng
authors.affiliation: Department of Anesthesiology, Beijing Tongren Hospital, Capital Medical University, Beijing 100730, P.R. China.
authors.forename: Xuezheng
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Department of Anesthesiology, Tianjin Hospital, Tianjin 300211, P.R. China.
authors.forename: Yongjin
authors.initials: Y
authors.lastname: He
authors.affiliation: Department of Neurobiology, Beijing Institute for Brain Disorders, Capital Medical University, Beijing 100069, P.R. China.
authors.forename: Junfa
authors.initials: J
authors.lastname: Li
authors.affiliation: Department of Anesthesiology, Beijing Tongren Hospital, Capital Medical University, Beijing 100730, P.R. China.
authors.forename: Tianzuo
authors.initials: T
authors.lastname: Li
mesh_terms: D000818:Animals; D001921:Brain / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D005663:Furans / Q000494:pharmacology / Q000627:therapeutic use*; D020244:Infarction, Middle Cerebral Artery / Q000150:complications / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D008297:Male; D008810:Mice, Inbred C57BL; D000066829:Neuroprotection / Q000187:drug effects; D018696:Neuroprotective Agents / Q000494:pharmacology / Q000627:therapeutic use*; D011493:Protein Kinase C / Q000378:metabolism*; D021381:Protein Transport / Q000187:drug effects*; D011753:Pyrones / Q000494:pharmacology / Q000627:therapeutic use*; D015427:Reperfusion Injury / Q000150:complications / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology
chemical_list: C502982:9-(benzoyloxy)-2-(3-furanyl)dodecahydro-6a,10b-dimethyl-4,10-dioxo-2H-naphtho(2,1-c)pyran-7-carboxylic acid methyl ester; D005663:Furans; D018696:Neuroprotective Agents; D011753:Pyrones; C087857:protein kinase C gamma; D011493:Protein Kinase C