title: Real-time detection and imaging of copper(ii) in cellular mitochondria.
abstract: A triphenylamine-pyridine derivative, viz., a fluorescence probe was designed and synthesized. The probe displayed an "on-off-on" fluorescence switch, upon the sequential addition of Cu2+and ethylenediaminetetraacetic acid (EDTA). The detection mechanism was confirmed by using UV-vis, fluorescence and1H NMR titration spectra. Interestingly, the probe can accumulate in mitochondria, and thus detect Cu2+in real-time there, which indicated that probe L can be used to monitor intracellular mitochondrial Cu2+levels. Further understanding of the biological functions of Cu2+in mitochondria has important implications for treating cancer and other diseases.
authors.affiliation: College of Chemistry and Chemical Engineering, Anhui University and Key Laboratory of Functional Inorganic Materials Chemistry of Anhui Province, 230601, Hefei, P.R. China. zhpzhp@263.net.
authors.forename: Hong
authors.initials: H
authors.lastname: Li
authors.affiliation: College of Chemistry and Chemical Engineering, Anhui University and Key Laboratory of Functional Inorganic Materials Chemistry of Anhui Province, 230601, Hefei, P.R. China. zhpzhp@263.net.
authors.forename: Ruilong
authors.initials: R
authors.lastname: Zhang
authors.affiliation: Centre of Stem cell Research and Transformation Medicine, Anhui University, Hefei 230601, P. R. China.
authors.forename: Chunxia
authors.initials: C
authors.lastname: Li
authors.affiliation: Centre of Stem cell Research and Transformation Medicine, Anhui University, Hefei 230601, P. R. China.
authors.forename: Bei
authors.initials: B
authors.lastname: Huang
authors.affiliation: College of Chemistry and Chemical Engineering, Anhui University and Key Laboratory of Functional Inorganic Materials Chemistry of Anhui Province, 230601, Hefei, P.R. China. zhpzhp@263.net.
authors.forename: Tingting
authors.initials: T
authors.lastname: Yu
authors.affiliation: College of Chemistry and Chemical Engineering, Anhui University and Key Laboratory of Functional Inorganic Materials Chemistry of Anhui Province, 230601, Hefei, P.R. China. zhpzhp@263.net.
authors.forename: Xiaodan
authors.initials: X
authors.lastname: Huang
authors.affiliation: Faculty of Health Science, University of Macau, Taipa, Macau SAR, P.R. China.
authors.forename: Xuanjun
authors.initials: X
authors.lastname: Zhang
authors.affiliation: College of Chemistry and Chemical Engineering, Anhui University and Key Laboratory of Functional Inorganic Materials Chemistry of Anhui Province, 230601, Hefei, P.R. China. zhpzhp@263.net.
authors.forename: Fei
authors.initials: F
authors.lastname: Li
authors.affiliation: College of Chemistry and Chemical Engineering, Anhui University and Key Laboratory of Functional Inorganic Materials Chemistry of Anhui Province, 230601, Hefei, P.R. China. zhpzhp@263.net.
authors.forename: Hongping
authors.identifier: http://orcid.org/0000-0003-3035-3328
authors.initials: H
authors.lastname: Zhou
authors.affiliation: College of Chemistry and Chemical Engineering, Anhui University and Key Laboratory of Functional Inorganic Materials Chemistry of Anhui Province, 230601, Hefei, P.R. China. zhpzhp@263.net.
authors.forename: Yupeng
authors.initials: Y
authors.lastname: Tian
mesh_terms: D002470:Cell Survival / Q000187:drug effects; D003300:Copper / Q000032:analysis* / Q000378:metabolism; D004492:Edetic Acid / Q000737:chemistry; D005456:Fluorescent Dyes / Q000737:chemistry* / Q000378:metabolism / Q000494:pharmacology; D056945:Hep G2 Cells; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008928:Mitochondria / Q000737:chemistry* / Q000378:metabolism; D015394:Molecular Structure; D061848:Optical Imaging; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet; D013997:Time Factors
chemical_list: D005456:Fluorescent Dyes; D003300:Copper; D004492:Edetic Acid