title: ClC-3 Promotes Osteogenic Differentiation in MC3T3-E1 Cell After Dynamic Compression.
abstract: ClC-3 chloride channel has been proved to have a relationship with the expression of osteogenic markers during osteogenesis, persistent static compression can upregulate the expression of ClC-3 and regulate osteodifferentiation in osteoblasts. However, there was no study about the relationship between the expression of ClC-3 and osteodifferentiation after dynamic compression. In this study, we applied dynamic compression on MC3T3-E1 cells to detect the expression of ClC-3, runt-related transcription factor 2 (Runx2), bone morphogenic protein-2 (BMP-2), osteopontin (OPN), nuclear-associated antigen Ki67 (Ki67), and proliferating cell nuclear antigen (PCNA) in biopress system, then we investigated the expression of these genes after dynamic compression with Chlorotoxin (specific ClC-3 chloride channel inhibitor) added. Under transmission electron microscopy, there were more cell surface protrusions, rough surfaced endoplasmic reticulum, mitochondria, Golgi apparatus, abundant glycogen, and lysosomes scattered in the cytoplasm in MC3T3-E1 cells after dynamic compression. The nucleolus was more obvious. We found that ClC-3 was significantly up-regulated after dynamic compression. The compressive force also up-regulated Runx2, BMP-2, and OPN after dynamic compression for 2, 4 and 8 h. The proliferation gene Ki67 and PCNA did not show significantly change after dynamic compression for 8 h. Chlorotoxin did not change the expression of ClC-3 but reduced the expression of Runx2, BMP-2, and OPN after dynamic compression compared with the group without Cltx added. The data from the current study suggested that ClC-3 may promotes osteogenic differentiation in MC3T3-E1 cell after dynamic compression. J. Cell. Biochem. 118: 1606-1613, 2017. © 2016 Wiley Periodicals, Inc.
authors.affiliation: Department of Stomatology, Third Hospital of Hebei Medical University, Shijiazhuang 050051, Hebei, China.
authors.forename: Dawei
authors.initials: D
authors.lastname: Wang
authors.affiliation: Department of Stomatology, Third Hospital of Hebei Medical University, Shijiazhuang 050051, Hebei, China.
authors.forename: Hao
authors.initials: H
authors.lastname: Wang
authors.affiliation: Department of Pathology, Third Hospital of Hebei Medical University, Shijiazhuang 050051, Hebei, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Gao
authors.affiliation: Department of Stomatology, Third Hospital of Hebei Medical University, Shijiazhuang 050051, Hebei, China.
authors.forename: Kun
authors.initials: K
authors.lastname: Wang
authors.affiliation: Department of Oral and Maxillofacial Surgery, Stomatology Hospital of Hebei Medical University, Shijiazhuang 050017, Hebei, China.|Hebei Key Laboratory of Oral Medicine, Shijiazhuang 050017, Hebei, China.
authors.forename: Fusheng
authors.initials: F
authors.lastname: Dong
mesh_terms: D000818:Animals; D055396:Bone Morphogenetic Protein 2 / Q000235:genetics; D002454:Cell Differentiation / Q000187:drug effects; D002460:Cell Line; D018118:Chloride Channels / Q000378:metabolism*; D050659:Core Binding Factor Alpha 1 Subunit / Q000235:genetics; D005786:Gene Expression Regulation / Q000187:drug effects; D051379:Mice; D010006:Osteoblasts / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D010012:Osteogenesis / Q000187:drug effects*; D053495:Osteopontin / Q000235:genetics; D012604:Scorpion Venoms / Q000494:pharmacology*; D013314:Stress, Mechanical; D015854:Up-Regulation
chemical_list: C525719:Bmp2 protein, mouse; D055396:Bone Morphogenetic Protein 2; D018118:Chloride Channels; C115376:ClC-3 channel; D050659:Core Binding Factor Alpha 1 Subunit; C493716:Runx2 protein, mouse; D012604:Scorpion Venoms; C091539:Chlorotoxin; D053495:Osteopontin
keywords: ClC-3 CHLORIDE CHANNEl; DYNAMIC COMPRESSION; OSTEOGENIC DIFFERENTIATION; THREE-DIMENSIONAL-OSTEOBLASTS MEMBRANE