title: DNA and BSA damage inhibitory activities, and anti-acetylcholinesterase, anti-porcine α-amylase and antioxidant properties of Dolichos lablab beans.
abstract: The underutilized Kenyan variety of Dolichos lablab bean seeds serve as a good source of natural antioxidants, which can probably be effective in reducing the risk of occurrence of several diseases. This study was undertaken for the first time to address the limited knowledge regarding the antioxidant activities of lablab beans. Moreover, their DNA damage inhibitory activity, bovine serum albumin (BSA) damage inhibitory activity, and the inhibition of acetylcholinesterase and porcine α-amylase were also investigated. The antioxidant capacity of Dolichos lablab bean seeds extracted with methanol, water or methanol/water combination was evaluated by the ferric-reducing antioxidant power (FRAP) assay, free radical-scavenging activity, 1,1-diphenyl-2-picrylhydrazyl (DPPH), nitric oxide (NO) radical-scavenging assay, and 2,20-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS). Results reported in the present study indicate that water, methanol and water/methanol extracts of lablab bean flour exhibited good antioxidant activity by effectively scavenging various free radicals, such as DPPH, NO, and ABTS radicals. The extracts also exhibited protective effects against DNA and BSA damage and inhibitory effects on porcine α-amylase. Findings of this study suggest that extracts from the lablab bean flour would have potential application in food supplements, and pharmaceutical and cosmetic industries.
authors.affiliation: Functional Foods and Nutraceuticals Laboratory (FFNL), Dairy Science and Technology Department, Faculty of Agriculture, Alexandria University, Alexandria, Egypt. Hosaml@hotmail.com.
authors.forename: Hosam M
authors.initials: HM
authors.lastname: Habib
authors.affiliation: Food and Nutrition Program, College of Nursing and Health Professions, University of Southern Indiana, 8600 University Boulevard, Evansville, IN, USA. Swtheuri@usi.edu.
authors.forename: Serah W
authors.initials: SW
authors.lastname: Theuri
authors.affiliation: Functional Foods and Nutraceuticals Laboratory (FFNL), Dairy Science and Technology Department, Faculty of Agriculture, Alexandria University, Alexandria, Egypt. Hosaml@hotmail.com.
authors.forename: Ehab
authors.initials: E
authors.lastname: Kheadr
authors.affiliation: College of Medicine and Health Sciences, United Arab Emirates University, P.O. Box 15551, Al Ain, United Arab Emirates.
authors.forename: Fedah E
authors.initials: FE
authors.lastname: Mohamed
mesh_terms: D000110:Acetylcholinesterase / Q000032:analysis; D000818:Animals; D002417:Cattle; D002800:Cholinesterase Inhibitors / Q000032:analysis*; D004247:DNA / Q000235:genetics; D004249:DNA Damage / Q000187:drug effects*; D031294:Dolichos / Q000737:chemistry*; D016166:Free Radical Scavengers / Q000032:analysis*; D010936:Plant Extracts / Q000032:analysis*; D012710:Serum Albumin, Bovine / Q000633:toxicity; D013552:Swine; D000516:alpha-Amylases / Q000032:analysis / Q000037:antagonists & inhibitors*
chemical_list: D002800:Cholinesterase Inhibitors; D016166:Free Radical Scavengers; D010936:Plant Extracts; D012710:Serum Albumin, Bovine; D004247:DNA; D000110:Acetylcholinesterase; D000516:alpha-Amylases