title: Evaluation of antioxidant activity of phenolic fractions from the leaves and petals of dandelion in human plasma treated with H2O2and H2O2/Fe.
abstract: Taraxacum officinale (dandelion) is a widespread perennial of the Asteraceae family. Dandelion is a rich source of different bioactive compounds, including phenolic compounds, terpenes, carbohydrates, proteins, fatty acids, vitamin and minerals. However, the content of phenolics in tested extracts by various authors was not always well described. Dandelion is also a commonly available food with a long history of human use and as such poses little risk of harm. In this study, we focused on four different phenolic fractions from leaves and petals of dandelion, which might be of great interest. The objective was to investigate the antioxidant properties of the phenolic fractions from dandelion leaves and petals in vitro. Effects of four different phenolic fractions from dandelion leaves and petals on the production of thiobarbituric acid reactive substances (TBARS, a marker of lipid peroxidation) in human plasma were studied in vitro. Their antioxidant properties against human plasma protein carbonylation and oxidation of protein thiols induced by a strong biological oxidant - hydrogen peroxide (H2O2) or H2O2/Fe (a donor of hydroxyl radicals) were also examined. The tested fractions of dandelion (0.5-50 μg/mL; the incubation time - 30 min) inhibited plasma lipid peroxidation induced by H2O2or H2O2/Fe. However, their antioxidant properties were not concentration-dependent. All tested samples also inhibited plasma protein carbonylation and oxidation of thiol groups in plasma proteins stimulated by oxidants (H2O2and OH∙). The obtained results suggest that four tested dandelion fractions, especially phenolic fractions from petals which are recognized as better than leaves source of flavonoids, may be a new and promising source of natural compounds with antioxidant activity beneficial for diseases-associated with oxidative stress, and with changes of hemostasis.
authors.affiliation: Department of Biochemistry and Crop Quality, Institute of Soil Science and Plant Cultivation, State Research Institute, 24-100 Puławy, Poland.
authors.forename: Dariusz
authors.initials: D
authors.lastname: Jędrejek
authors.affiliation: Department of General Biochemistry, Faculty of Biology and Environmental Protection, University of Łódź, 90-236 Łódź, Poland.
authors.forename: Bogdan
authors.initials: B
authors.lastname: Kontek
authors.affiliation: Department of General Biochemistry, Faculty of Biology and Environmental Protection, University of Łódź, 90-236 Łódź, Poland.
authors.forename: Bernadetta
authors.initials: B
authors.lastname: Lis
authors.affiliation: Department of Biochemistry and Crop Quality, Institute of Soil Science and Plant Cultivation, State Research Institute, 24-100 Puławy, Poland.
authors.forename: Anna
authors.initials: A
authors.lastname: Stochmal
authors.affiliation: Department of General Biochemistry, Faculty of Biology and Environmental Protection, University of Łódź, 90-236 Łódź, Poland. Electronic address: olasb@biol.uni.lodz.pl.
authors.forename: Beata
authors.initials: B
authors.lastname: Olas
mesh_terms: D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology; D001798:Blood Proteins / Q000737:chemistry / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D035264:Flowers / Q000737:chemistry / Q000378:metabolism; D006801:Humans; D006861:Hydrogen Peroxide / Q000633:toxicity*; D017665:Hydroxyl Radical / Q000633:toxicity*; D015227:Lipid Peroxidation / Q000187:drug effects; D018384:Oxidative Stress / Q000187:drug effects*; D010636:Phenols / Q000737:chemistry / Q000494:pharmacology*; D010936:Plant Extracts / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry / Q000378:metabolism; D050050:Protein Carbonylation / Q000187:drug effects; D021241:Spectrometry, Mass, Electrospray Ionization; D031205:Taraxacum / Q000737:chemistry* / Q000378:metabolism
chemical_list: D000975:Antioxidants; D001798:Blood Proteins; D010636:Phenols; D010936:Plant Extracts; D017665:Hydroxyl Radical; D006861:Hydrogen Peroxide
keywords: Antioxidant activity; Dandelion; Lipid peroxidation; Oxidative stress