title: tRNA-Derived RNA Fragments Associate with Human Multisynthetase Complex (MSC) and Modulate Ribosomal Protein Translation.
abstract: The functionality of small RNAs from abundant species of "housekeeping" noncoding RNAs (e.g., rRNA, tRNA, snRNA, snoRNA, etc.) remains a highly studied topic. The current state of research on short RNAs derived from transfer RNA (tRNA), called tRNA-derived fragments (tRFs), has been restricted largely to expression studies and limited functional studies. 5' tRFs are known translational inhibitors in mammalian cells, yet little is known about their functionality. Here we report on the first experimental evidence of the tRF protein interactome, identifying the mammalian multisynthetase complex as the primary interactor of the 5' tRF Gln19. We also present proteome-wide SILAC evidence that 5' tRFs increase ribosomal and poly(A)-binding protein translation.
authors.affiliation: Centre for Health Technologies, Faculty of Engineering and Information Technology, University of Technology Sydney , Ultimo 2007, Australia.
authors.forename: Simon P
authors.identifier: 0000-0001-9053-9138
authors.initials: SP
authors.lastname: Keam
authors.affiliation: Wellcome Trust Centre for Gene Regulation and Expression, University of Dundee , Dundee DD1 4HN, United Kingdom.
authors.forename: Andrew
authors.initials: A
authors.lastname: Sobala
authors.affiliation: Wellcome Trust Centre for Gene Regulation and Expression, University of Dundee , Dundee DD1 4HN, United Kingdom.
authors.forename: Sara
authors.initials: S
authors.lastname: Ten Have
authors.affiliation: Centre for Health Technologies, Faculty of Engineering and Information Technology, University of Technology Sydney , Ultimo 2007, Australia.
authors.forename: Gyorgy
authors.initials: G
authors.lastname: Hutvagner
mesh_terms: D001483:Base Sequence; D019295:Computational Biology; D047468:Immunoprecipitation; D007553:Isotope Labeling; D008025:Ligases / Q000235:genetics* / Q000378:metabolism; D009097:Multienzyme Complexes / Q000235:genetics* / Q000378:metabolism; D011061:Poly A / Q000235:genetics / Q000378:metabolism; D014176:Protein Biosynthesis; D012343:RNA, Transfer / Q000235:genetics* / Q000378:metabolism; D022661:RNA, Untranslated / Q000235:genetics / Q000378:metabolism; D016601:RNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D012270:Ribosomes / Q000235:genetics* / Q000378:metabolism
chemical_list: D009097:Multienzyme Complexes; D022661:RNA, Untranslated; D016601:RNA-Binding Proteins; D011061:Poly A; D012343:RNA, Transfer; D008025:Ligases
keywords: SILAC; immunoprecipitation; mass spectrometry; multisynthetase complex; protein translation; small RNA; tRF; tRNA-derived fragment