title: Treatment of Helicobacter pylori infected mice with Bryophyllum pinnatum, a medicinal plant with antioxidant and antimicrobial properties, reduces bacterial load.
abstract: BACKGROUND
  Bryophyllum pinnatum (Lam.) Kurz (Crassulaceae) is a plant known for its antiulcer properties.
  
  
  OBJECTIVE
  This study evaluates the anti-Helicobacter pylori activity of Bryophyllum pinnutum methanol extract with a mouse model and its antioxidant properties.
  
  
  METHODS
  Dried leaves of Bryophyllum pinnutum were extracted with methanol and ethyl acetate. Broth microdilution method was used to evaluate the anti-Helicobacter activity of extract samples in vitro. Swiss mice were inoculated with a suspension of Helicobacter pylori and divided into control group and four others that received 125, 250, 500 mg/kg of methanol extract or ciprofloxacin (500 mg/kg), respectively, for 7 days. Helicobacter pylori colonization and bacterial load of mouse stomach was assessed on day 1 and 7 post-treatment. The antioxidant activity of Bryophyllum pinnutum was evaluated through DPPH radical, hydroxyl radical and reducing power assay.
  
  
  RESULTS
  Methanol extract showed a significant anti-Helicobacter activity with MIC and MBC values of 32 and 256 μg/mL, respectively. Bryophyllum pinnatum and ciprofloxacin reduced H. pylori colonization of gastric tissue from 100% to 17%. Bryophyllum pinnatum extract (85.91 ± 52.91 CFU) and standard (25.74 ± 16.15 CFU) also reduced significantly (p < 0.05) bacterial load of gastric mucosa as compared to untreated infected mice (11883 ± 1831 CFU). DPPH radical, hydroxyl radical and reducing power assays showed IC50values of 25.31 ± 0.34, 55.94 ± 0.68 and 11.18 ± 0.74 μg/mL, respectively.
  
  
  CONCLUSIONS
  The data suggest that the methanol extract of Bryophyllum pinnatum could inhibit Helicobacter pylori growth, and may also acts as an antioxidant to protect gastric mucosa against reactive oxygen species.
authors.affiliation: a Microbiology and Pharmacology Laboratory, Department of Biochemistry, Faculty of Science , University of Dschang , Dschang , Cameroon.
authors.forename: Laure Brigitte
authors.initials: LB
authors.lastname: Kouitcheu Mabeku
authors.affiliation: a Microbiology and Pharmacology Laboratory, Department of Biochemistry, Faculty of Science , University of Dschang , Dschang , Cameroon.
authors.forename: Bertrand
authors.initials: B
authors.lastname: Eyoum Bille
authors.affiliation: a Microbiology and Pharmacology Laboratory, Department of Biochemistry, Faculty of Science , University of Dschang , Dschang , Cameroon.
authors.forename: Thibau Flaurant
authors.initials: TF
authors.lastname: Tchouangueu
authors.affiliation: b Gastroenterology Department , Laquintinie Hospital of Douala , Douala , Cameroon.
authors.forename: Eveline
authors.initials: E
authors.lastname: Nguepi
authors.affiliation: b Gastroenterology Department , Laquintinie Hospital of Douala , Douala , Cameroon.
authors.forename: Hubert
authors.initials: H
authors.lastname: Leundji
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000302:isolation & purification / Q000494:pharmacology*; D000975:Antioxidants / Q000302:isolation & purification / Q000494:pharmacology*; D058491:Bacterial Load; D001713:Biphenyl Compounds / Q000737:chemistry; D004195:Disease Models, Animal; D005753:Gastric Mucosa / Q000187:drug effects* / Q000382:microbiology; D016481:Helicobacter Infections / Q000188:drug therapy* / Q000382:microbiology; D016480:Helicobacter pylori / Q000187:drug effects* / Q000472:pathogenicity; D017665:Hydroxyl Radical / Q000737:chemistry; D031962:Kalanchoe / Q000737:chemistry*; D000432:Methanol / Q000737:chemistry; D051379:Mice; D008826:Microbial Sensitivity Tests; D010084:Oxidation-Reduction; D008517:Phytotherapy; D010851:Picrates / Q000737:chemistry; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology*; D018515:Plant Leaves; D010946:Plants, Medicinal; D012997:Solvents / Q000737:chemistry; D013997:Time Factors
chemical_list: D000900:Anti-Bacterial Agents; D000975:Antioxidants; D001713:Biphenyl Compounds; D010851:Picrates; D010936:Plant Extracts; D012997:Solvents; D017665:Hydroxyl Radical; C004931:1,1-diphenyl-2-picrylhydrazyl; D000432:Methanol
keywords: Gastroduodenal infection; anti-Helicobacter pylori; herbal drug