title: Noninvasive Microsurgery Using Aptamer-Functionalized Magnetic Microdisks for Tumor Cell Eradication.
abstract: Magnetomechanical cell disruption using nano- and microsized structures is a promising biomedical technology used for noninvasive elimination of diseased cells. It applies alternating magnetic field (AMF) for ferromagnetic microdisks making them oscillate and causing cell membrane disruption with cell death followed by apoptosis. In this study, we functionalized the magnetic microdisks with cell-binding DNA aptamers and guided the microdisks to recognize cancerous cells in a mouse tumor in vivo. Only 10 min of the treatment with a 100 Hz AMF was enough to eliminate cancer cells from a malignant tumor. Our results demonstrate a good perspective of using aptamer-modified magnetic microdisks for noninvasive microsurgery for tumors.
authors.affiliation: 1 Laboratory of Biomolecular and Medical Technologies, Krasnoyarsk State Medical University , Krasnoyarsk, Russia .|2 Siberian Federal University , Krasnoyarsk, Russia .
authors.forename: Tatiana N
authors.initials: TN
authors.lastname: Zamay
authors.affiliation: 1 Laboratory of Biomolecular and Medical Technologies, Krasnoyarsk State Medical University , Krasnoyarsk, Russia .|3 Krasnoyarsk Research Center, Siberian Branch of the Russian Academy of Sciences , Krasnoyarsk, Russia .
authors.forename: Galina S
authors.initials: GS
authors.lastname: Zamay
authors.affiliation: 1 Laboratory of Biomolecular and Medical Technologies, Krasnoyarsk State Medical University , Krasnoyarsk, Russia .|2 Siberian Federal University , Krasnoyarsk, Russia .
authors.forename: Irina V
authors.initials: IV
authors.lastname: Belyanina
authors.affiliation: 3 Krasnoyarsk Research Center, Siberian Branch of the Russian Academy of Sciences , Krasnoyarsk, Russia .
authors.forename: Sergey S
authors.initials: SS
authors.lastname: Zamay
authors.affiliation: 3 Krasnoyarsk Research Center, Siberian Branch of the Russian Academy of Sciences , Krasnoyarsk, Russia .|4 Institute of Computational Modeling, Siberian Branch of the Russian Academy of Sciences , Krasnoyarsk, Russia .
authors.forename: Valery V
authors.initials: VV
authors.lastname: Denisenko
authors.affiliation: 1 Laboratory of Biomolecular and Medical Technologies, Krasnoyarsk State Medical University , Krasnoyarsk, Russia .|3 Krasnoyarsk Research Center, Siberian Branch of the Russian Academy of Sciences , Krasnoyarsk, Russia .
authors.forename: Olga S
authors.initials: OS
authors.lastname: Kolovskaya
authors.affiliation: 3 Krasnoyarsk Research Center, Siberian Branch of the Russian Academy of Sciences , Krasnoyarsk, Russia .
authors.forename: Tatiana I
authors.initials: TI
authors.lastname: Ivanchenko
authors.affiliation: 1 Laboratory of Biomolecular and Medical Technologies, Krasnoyarsk State Medical University , Krasnoyarsk, Russia .|2 Siberian Federal University , Krasnoyarsk, Russia .
authors.forename: Valentina L
authors.initials: VL
authors.lastname: Grigorieva
authors.affiliation: 2 Siberian Federal University , Krasnoyarsk, Russia .|3 Krasnoyarsk Research Center, Siberian Branch of the Russian Academy of Sciences , Krasnoyarsk, Russia .
authors.forename: Irina V
authors.initials: IV
authors.lastname: Garanzha
authors.affiliation: 1 Laboratory of Biomolecular and Medical Technologies, Krasnoyarsk State Medical University , Krasnoyarsk, Russia .|3 Krasnoyarsk Research Center, Siberian Branch of the Russian Academy of Sciences , Krasnoyarsk, Russia .
authors.forename: Dmitry V
authors.initials: DV
authors.lastname: Veprintsev
authors.affiliation: 1 Laboratory of Biomolecular and Medical Technologies, Krasnoyarsk State Medical University , Krasnoyarsk, Russia .
authors.forename: Yury E
authors.initials: YE
authors.lastname: Glazyrin
authors.affiliation: 3 Krasnoyarsk Research Center, Siberian Branch of the Russian Academy of Sciences , Krasnoyarsk, Russia .
authors.forename: Alexandr V
authors.initials: AV
authors.lastname: Shabanov
authors.affiliation: 5 The Institute of Semiconductor Physics, Siberian Branch of the Russian Academy of Sciences , Novosibirsk, Russia .
authors.forename: Viktor Y
authors.initials: VY
authors.lastname: Prinz
authors.affiliation: 5 The Institute of Semiconductor Physics, Siberian Branch of the Russian Academy of Sciences , Novosibirsk, Russia .
authors.forename: Vladimir A
authors.initials: VA
authors.lastname: Seleznev
authors.affiliation: 6 Institute of Physics, Siberian Branch of the Russian Academy of Sciences , Krasnoyarsk, Russia .
authors.forename: Alexey E
authors.initials: AE
authors.lastname: Sokolov
authors.affiliation: 7 Krasnoyarsk State Pedagogical University , Krasnoyarsk, Russia .
authors.forename: Vladimir S
authors.initials: VS
authors.lastname: Prokopenko
authors.affiliation: 3 Krasnoyarsk Research Center, Siberian Branch of the Russian Academy of Sciences , Krasnoyarsk, Russia .
authors.forename: Petr D
authors.initials: PD
authors.lastname: Kim
authors.affiliation: 8 Department of Chemistry and Biomolecular Sciences, University of Ottawa , Ottawa, Canada .
authors.forename: Ana
authors.initials: A
authors.lastname: Gargaun
authors.affiliation: 8 Department of Chemistry and Biomolecular Sciences, University of Ottawa , Ottawa, Canada .
authors.forename: Maxim V
authors.initials: MV
authors.lastname: Berezovski
authors.affiliation: 1 Laboratory of Biomolecular and Medical Technologies, Krasnoyarsk State Medical University , Krasnoyarsk, Russia .|3 Krasnoyarsk Research Center, Siberian Branch of the Russian Academy of Sciences , Krasnoyarsk, Russia .
authors.forename: Anna S
authors.initials: AS
authors.lastname: Zamay
mesh_terms: D000818:Animals; D052157:Aptamers, Nucleotide / Q000138:chemical synthesis / Q000378:metabolism*; D002286:Carcinoma, Ehrlich Tumor / Q000378:metabolism / Q000473:pathology / Q000628:therapy*; D005353:Fibronectins / Q000378:metabolism; D064448:Filamins / Q000378:metabolism; D015552:Injections, Intralesional; D055909:Magnetic Field Therapy / Q000295:instrumentation / Q000379:methods*; D060526:Magnetic Fields; D059346:Magnets; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D008866:Microsurgery / Q000379:methods*; D009368:Neoplasm Transplantation; D011485:Protein Binding; D013438:Sulfhydryl Compounds / Q000737:chemistry
chemical_list: D052157:Aptamers, Nucleotide; D005353:Fibronectins; D064448:Filamins; D013438:Sulfhydryl Compounds
keywords: aptamer; magnetic microdisks; magnetodynamic therapy; tumor eradication