title: A Cost-Effective Method to Assemble Biomimetic 3D Cell Culture Platforms.
abstract: METHODS
  We utilized the hAM to provide the biological and the three dimensional (3D) topographic components of the prototype. The 3D nano-roughness of the hAM was characterized using surface electron microscopy and surface image analysis (ImageJ and SurfaceJ). We developed additional macro-scale and micro-scale versions of the platform which provided additional shear stress factors to simulate the fluid dynamics of the in vivo extracellular fluids.
  
  
  RESULTS
  Three models of varying complexities of the prototype were assembled. A well-defined 3D surface modulation of the hAM in comparable to commercial 3D biomaterial culture substrates was achieved without complex fabrication and with significantly lower cost. Performance of the prototype was demonstrated through culture of primary human umbilical cord mononuclear blood cells (MNCs), human bone marrow mesenchymal stem cell line (hBMSC), and human breast cancer tissue.
  
  
  CONCLUSIONS
  This study presents methods of assembling an integrated, flexible and low cost biomimetic cell culture platform for diverse cell culture applications.
authors.affiliation: Center of Excellence for Stem Cells and Regenerative Medicine, Zewail City of Science and Technology, Giza, Egypt.
authors.forename: Sabreen
authors.initials: S
authors.lastname: Khalil
authors.affiliation: Center of Excellence for Stem Cells and Regenerative Medicine, Zewail City of Science and Technology, Giza, Egypt.|University of Science and Technology, Zewail City of Science and Technology, Giza, Egypt.
authors.forename: Nagwa
authors.initials: N
authors.lastname: El-Badri
authors.affiliation: Department of Microbiology and Immunology, Faculty of Medicine, Assiut University, Assiut, Egypt.
authors.forename: Mohamed
authors.initials: M
authors.lastname: El-Mokhtaar
authors.affiliation: Center of Excellence for Stem Cells and Regenerative Medicine, Zewail City of Science and Technology, Giza, Egypt.|University of Science and Technology, Zewail City of Science and Technology, Giza, Egypt.
authors.forename: Saif
authors.initials: S
authors.lastname: Al-Mofty
authors.affiliation: Center of Excellence for Stem Cells and Regenerative Medicine, Zewail City of Science and Technology, Giza, Egypt.
authors.forename: Mohamed
authors.initials: M
authors.lastname: Farghaly
authors.affiliation: Center of Excellence for Stem Cells and Regenerative Medicine, Zewail City of Science and Technology, Giza, Egypt.
authors.forename: Radwa
authors.initials: R
authors.lastname: Ayman
authors.affiliation: Department of Obstetrics and Gynecology, Faculty of Medicine, Assiut University, Assiut, Egypt.
authors.forename: Dina
authors.initials: D
authors.lastname: Habib
authors.affiliation: University of Science and Technology, Zewail City of Science and Technology, Giza, Egypt.
authors.forename: Noha
authors.initials: N
authors.lastname: Mousa
mesh_terms: D000650:Amnion / Q000737:chemistry* / Q000648:ultrastructure; D040761:Biomimetic Materials / Q000302:isolation & purification* / Q000494:pharmacology; D001943:Breast Neoplasms / Q000473:pathology; D018929:Cell Culture Techniques / Q000191:economics / Q000379:methods*; D002454:Cell Differentiation / Q000187:drug effects; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D003362:Cost-Benefit Analysis; D005260:Female; D005312:Fetal Blood / Q000166:cytology; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000166:cytology / Q000187:drug effects; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000187:drug effects; D008855:Microscopy, Electron, Scanning; D015203:Reproducibility of Results; D046509:Tissue Culture Techniques