title: C-type natriuretic peptide prevents kidney injury and attenuates oxidative and inflammatory responses in hemorrhagic shock.
abstract: Oxidative stress induced by hemorrhagic shock (HS) initiates a systemic inflammatory response, which leads to subsequent kidney injury. This study assessed the efficacy of c-type natriuretic peptide (CNP) in attenuating kidney injury in a rat model of hemorrhagic shock and resuscitation (HS/R). Sodium pentobarbital-anesthetized adult male Wistar rats underwent HS induced by the withdrawal of blood to a mean arterial pressure of 30-35 mmHg for 50 min. Then, the animals received CNP (25 μg/kg) or vehicle (saline) intravenously, followed byresuscitation with 1.5 times the shed blood volume in the form of normal saline. Mean arterial pressure was measured throughout the experiment, and acid-base status, oxidative stress, inflammation, tissue injury and kidney function were evaluated after resuscitation. CNP infusion reduced the malondialdehyde content, lowered the myeloperoxidase activity and decreased the expression of tumor necrosis factor (TNF)-α, interleukin (IL)-6, and IL-1β in the kidney. The histologic injury score and the plasma creatinine concentration were also significantly decreased after CNP treatment compared to the vehicle group. CNP treatment ameliorates oxidative stress, the inflammatory response, and consequently acute kidney injury after HS/R. Thus, CNP may represent a promising strategy to improve resuscitation for the treatment of HS and deserves further investigation.
authors.affiliation: Institute of Transfusion Medicine, Academy of Military Medical Sciences, 27th Taiping Road, Beijing, 100850, People's Republic of China.
authors.forename: Gan
authors.initials: G
authors.lastname: Chen
authors.affiliation: Institute of Transfusion Medicine, Academy of Military Medical Sciences, 27th Taiping Road, Beijing, 100850, People's Republic of China.
authors.forename: Xiang
authors.initials: X
authors.lastname: Song
authors.affiliation: Institute of Transfusion Medicine, Academy of Military Medical Sciences, 27th Taiping Road, Beijing, 100850, People's Republic of China.
authors.forename: Yujing
authors.initials: Y
authors.lastname: Yin
authors.affiliation: Institute of Transfusion Medicine, Academy of Military Medical Sciences, 27th Taiping Road, Beijing, 100850, People's Republic of China.
authors.forename: Sha
authors.initials: S
authors.lastname: Xia
authors.affiliation: Institute of Transfusion Medicine, Academy of Military Medical Sciences, 27th Taiping Road, Beijing, 100850, People's Republic of China.
authors.forename: Qingjun
authors.initials: Q
authors.lastname: Liu
authors.affiliation: Institute of Transfusion Medicine, Academy of Military Medical Sciences, 27th Taiping Road, Beijing, 100850, People's Republic of China.
authors.forename: Guoxing
authors.initials: G
authors.lastname: You
authors.affiliation: Institute of Transfusion Medicine, Academy of Military Medical Sciences, 27th Taiping Road, Beijing, 100850, People's Republic of China. zhaolian@bmi.ac.cn.
authors.forename: Lian
authors.initials: L
authors.lastname: Zhao
authors.affiliation: Institute of Transfusion Medicine, Academy of Military Medical Sciences, 27th Taiping Road, Beijing, 100850, People's Republic of China. zhouhtt1966@163.com.
authors.forename: Hong
authors.initials: H
authors.lastname: Zhou
mesh_terms: D058186:Acute Kidney Injury / Q000378:metabolism / Q000517:prevention & control*; D000818:Animals; D001784:Blood Gas Analysis; D016207:Cytokines / Q000378:metabolism; D004195:Disease Models, Animal; D007249:Inflammation / Q000188:drug therapy; D007668:Kidney / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D020098:Natriuretic Peptide, C-Type / Q000494:pharmacology*; D009504:Neutrophils / Q000187:drug effects / Q000473:pathology; D018384:Oxidative Stress / Q000187:drug effects*; D017208:Rats, Wistar; D012151:Resuscitation; D012771:Shock, Hemorrhagic / Q000150:complications / Q000188:drug therapy*
chemical_list: D016207:Cytokines; D020098:Natriuretic Peptide, C-Type
keywords: Acute kidney injury; C-type natriuretic peptide; Hemorrhagic shock; Inflammatory response; Oxidative stress