title: Mechanobiological implications of articular cartilage crystals.
abstract: Calcium crystals exist in both pathological and normal articular cartilage. The prevalence of these crystals dramatically increases with age, and crystals are typically found in osteoarthritic cartilage and synovial fluid. Relatively few studies have examined the effects of crystals on cartilage biomechanics or chondrocyte mechanotransduction. The purpose of this review is to describe how crystals could influence cartilage biomechanics and mechanotransduction in osteoarthritis.
  
  
  
  Crystals are found in both loaded and unloaded regions of articular cartilage. Exogenous crystals, in combination with joint motion, result in substantial joint inflammation. Articular cartilage vesicles promote crystal formation, and these vesicles are found near the periphery of chondrocytes. Crystallographic studies report monoclinic symmetry for synthetic crystals, suggesting that crystals will have a large stiffness compared with the cartilage extracellular matrix, the pericellular matrix, or the chondrocyte. This stiffness imbalance may cause crystal-induced dysregulation of chondrocyte mechanotransduction promoting both aging and osteoarthritis chondrocyte phenotypes.
  
  
  
  Because of their high stiffness compared with cartilage matrix, crystals likely alter chondrocyte mechanotransduction, and high concentrations of crystals within cartilage may alter macroscale biomechanics. Future studies should focus on understanding the mechanical properties of joint crystals and developing methods to understand how crystals affect chondrocyte mechanotransduction.
authors.affiliation: aDepartment of Cell Biology & Neuroscience bDepartment of Mechanical & Industrial Engineering, Montana State University, Bozeman, Montana, USA.
authors.forename: Alyssa K
authors.initials: AK
authors.lastname: Carlson
authors.forename: Carley N
authors.initials: CN
authors.lastname: McCutchen
authors.forename: Ronald K
authors.initials: RK
authors.lastname: June
mesh_terms: D002130:Calcium Phosphates / Q000378:metabolism*; D002131:Calcium Pyrophosphate / Q000378:metabolism*; D002358:Cartilage, Articular / Q000166:cytology / Q000378:metabolism* / Q000503:physiopathology; D002805:Chondrocalcinosis / Q000378:metabolism* / Q000503:physiopathology; D019902:Chondrocytes / Q000166:cytology / Q000378:metabolism*; D005109:Extracellular Matrix / Q000378:metabolism*; D006801:Humans; D040542:Mechanotransduction, Cellular / Q000502:physiology; D010003:Osteoarthritis / Q000378:metabolism* / Q000503:physiopathology; D013314:Stress, Mechanical; D016474:Weight-Bearing
chemical_list: D002130:Calcium Phosphates; C020243:calcium phosphate; D002131:Calcium Pyrophosphate