title: [Tryptase inhibits cell apoptosis through upregulating PAR-2 and Rho kinase in rheumatoid arthritis synovial fibroblasts].
abstract: Objective To investigate the regulatory effects of tryptase on protease-activated receptors 2 (PAR-2), Rho signal pathway and apoptosis of MH7A rheumatoid arthritis synovial fibroblasts. Methods In MH7A cells, the expression of PAR-2 was measured by flow cytometry; cell apoptosis was examined by annexin V- FITC/PI staining combined with flow cytometry; the expression of Rho kinase was detected by Pull-down assay and Western botting. Results Tryptase upregulated the expression of PAR-2 in MH7A cells, and suppressed Fas-mediated apoptosis of MH7A cells in a dose-dependent manner. Meanwhile, PAR-2 inhibitor, FSLLRY-NH2 significantly reduced anti-apoptotic effects of tryptase in MH7A cells, which was related with the increase of activated Rho kinase expression. Conclusion Tryptase plays a role in resistance to the apoptosis of MH7A cells through raising PAR-2 and activating Rho kinase.
authors.affiliation: Medical College of China Three Gorges University, Yichang 443002, China.
authors.forename: Qianqian
authors.initials: Q
authors.lastname: Zheng
authors.affiliation: Medical College of China Three Gorges University, Yichang 443002, China. *Corresponding author, E-mail: fox201@163.com.
authors.forename: Shigang
authors.initials: S
authors.lastname: Li
authors.affiliation: Medical College of China Three Gorges University, Yichang 443002, China.
authors.forename: Yunli
authors.initials: Y
authors.lastname: Jia
authors.affiliation: Medical College of China Three Gorges University, Yichang 443002, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Liu
authors.affiliation: Medical College of China Three Gorges University, Yichang 443002, China.
authors.forename: Lingling
authors.initials: L
authors.lastname: Yu
authors.affiliation: Medical College of China Three Gorges University, Yichang 443002, China.
authors.forename: Xianyong
authors.initials: X
authors.lastname: Chen
authors.affiliation: Medical College of China Three Gorges University, Yichang 443002, China.
authors.forename: Jinling
authors.initials: J
authors.lastname: Wang
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D001172:Arthritis, Rheumatoid / Q000378:metabolism*; D002478:Cells, Cultured; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism*; D005434:Flow Cytometry; D006801:Humans; D009842:Oligopeptides / Q000494:pharmacology; D044464:Receptor, PAR-2 / Q000037:antagonists & inhibitors / Q000378:metabolism*; D013583:Synovial Membrane / Q000166:cytology*; D053802:Tryptases / Q000494:pharmacology*; D054460:rho-Associated Kinases / Q000378:metabolism*
chemical_list: C508716:H-Phe-Ser-Leu-Leu-Arg-Tyr-NH2; D009842:Oligopeptides; D044464:Receptor, PAR-2; D054460:rho-Associated Kinases; D053802:Tryptases