title: Recombinant DNA vaccine of Hantavirus Gn and LAMP1 induced long-term immune protection in mice.
abstract: Prophylaxis is widely adopted the best choice against Hemorrhagic fever with renal syndrome (HFRS) caused by Hantavirus. However, loss of memory immune response maintenance remains as major shortcoming in current HFRS vaccine. A recombinant DNA vaccine, pVAX-LAMP/Gn was previously proved efficient, requiring long-term evaluations.
  
  
  
  Immune responses of Balb/c mice were assessed by specific and neutralizing antibodies, interferon-γ ELISpot assay, and cytotoxic T-lymphocyte cytotoxicity assay. HTNV-challenge assay identified long-term protection. Safety was confirmed by histological and behavioral analysis. Epitope-spreading phenomenon was noted, revealing two sets of dominant T-cell epitopes cross-species.
  
  
  
  pVAX-LAMP/Gn established memory responses within a long-term protection. Lysosome-targeted strategy showed promise on Gn-based DNA vaccine and further investigations are warranted in other immunogenic Hantaviral antigens.
authors.affiliation: Department of Immunology, Fourth Military Medical University, No.169, Changle W. Rd., Xi'an, 710032, China.
authors.forename: Dong-Bo
authors.initials: DB
authors.lastname: Jiang
authors.affiliation: Department of Ophthalmology, Xijing Hospital, Fourth Military Medical University, No.169, Changle W. Rd., Xi'an, 710032, China.
authors.forename: Li-Juan
authors.initials: LJ
authors.lastname: Sun
authors.affiliation: Department of Microbiology, Fourth Military Medical University, No.169, Changle W. Rd., Xi'an, 710032, China.
authors.forename: Lin-Feng
authors.initials: LF
authors.lastname: Cheng
authors.affiliation: Department of Immunology, Fourth Military Medical University, No.169, Changle W. Rd., Xi'an, 710032, China.
authors.forename: Jin-Peng
authors.initials: JP
authors.lastname: Zhang
authors.affiliation: Department of Immunology, Fourth Military Medical University, No.169, Changle W. Rd., Xi'an, 710032, China.
authors.forename: Shao-Bo
authors.initials: SB
authors.lastname: Xiao
authors.affiliation: Department of Immunology, Fourth Military Medical University, No.169, Changle W. Rd., Xi'an, 710032, China.
authors.forename: Yuan-Jie
authors.initials: YJ
authors.lastname: Sun
authors.affiliation: Department of Immunology, Fourth Military Medical University, No.169, Changle W. Rd., Xi'an, 710032, China.
authors.forename: Shu-Ya
authors.initials: SY
authors.lastname: Yang
authors.affiliation: Department of Immunology, Fourth Military Medical University, No.169, Changle W. Rd., Xi'an, 710032, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Wang
authors.affiliation: Department of Microbiology, Fourth Military Medical University, No.169, Changle W. Rd., Xi'an, 710032, China. Electronic address: flzhang@fmmu.edu.cn.
authors.forename: Fang-Lin
authors.initials: FL
authors.lastname: Zhang
authors.affiliation: Department of Immunology, Fourth Military Medical University, No.169, Changle W. Rd., Xi'an, 710032, China. Electronic address: yangkunkun@fmmu.edu.cn.
authors.forename: Kun
authors.initials: K
authors.lastname: Yang
mesh_terms: D000818:Animals; D057134:Antibodies, Neutralizing / Q000097:blood; D000914:Antibodies, Viral / Q000097:blood; D004247:DNA; D004274:DNA, Recombinant / Q000276:immunology; D058501:Enzyme-Linked Immunospot Assay; D006476:Orthohantavirus / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D018778:Hantavirus Infections / Q000517:prevention & control*; D007156:Immunologic Memory; D051907:Lysosomal Membrane Proteins / Q000235:genetics* / Q000276:immunology; D008562:Membrane Glycoproteins / Q000008:administration & dosage / Q000235:genetics* / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D019444:Vaccines, DNA / Q000008:administration & dosage / Q000276:immunology*; D014764:Viral Proteins / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology; D014765:Viral Vaccines / Q000276:immunology*; D052118:Lysosomal-Associated Membrane Protein 1
chemical_list: D057134:Antibodies, Neutralizing; D000914:Antibodies, Viral; D004247:DNA; D004274:DNA, Recombinant; D051907:Lysosomal Membrane Proteins; D008562:Membrane Glycoproteins; D019444:Vaccines, DNA; D014764:Viral Proteins; D014765:Viral Vaccines; C052255:Lamp1 protein, mouse; D052118:Lysosomal-Associated Membrane Protein 1
keywords: DNA vaccine; Glycoprotein N-terminal; Hantavirus; Lysosome-associated membrane protein; Memory immune response