title: Bioactive chemical constituents from the root bark of Morus australis.
abstract: Two new pyranoflavonoids, morustralins A (1) and B (2), a new natural benzene derivative, one benzenoid (Z)-1-hydroxy-4-(2-nitroethenyl)benzene (3), and thirty known compounds were isolated and characterized from the root bark of Morus australis. The structures of the new compounds were established from spectroscopic and spectrometric analyses. Ten isolates (1-10) were examined for inhibitory effects on adenosine diphosphate (ADP)-, arachidonic acid (AA)-, and platelet-aggregating factor (PAF)-induced platelet aggregation. Among the tested compounds, compound 3 displayed the most significant inhibition of ADP- and AA-induced platelet aggregation with IC50values of 9.76±5.54 and 9.81±2.7μM, respectively. In addition, eight purified compounds (3-10) were examined for inhibition of nitric oxide (NO) production in RAW 264.7 cells and six compounds (3-8) displayed significant inhibitory effects with IC50values ranging from 2.1±0.3 to 6.3±0.6μM.
authors.affiliation: School of Pharmacy, National Cheng Kung University Hospital, College of Medicine, National Cheng Kung University, Tainan 701, Taiwan, ROC.
authors.forename: Yu-Ren
authors.initials: YR
authors.lastname: Liao
authors.affiliation: School of Pharmacy, National Cheng Kung University Hospital, College of Medicine, National Cheng Kung University, Tainan 701, Taiwan, ROC.
authors.forename: Ping-Chung
authors.initials: PC
authors.lastname: Kuo
authors.affiliation: Division of Chinese Medicine Literature and Informatics, National Research Institute of Chinese Medicine, Taipei 112, Taiwan, ROC.
authors.forename: Wei-Jern
authors.initials: WJ
authors.lastname: Tsai
authors.affiliation: Department of Chinese Pharmaceutical Sciences and Chinese Medicine Resources, China Medical University, Taichung 404, Taiwan, ROC.
authors.forename: Guan-Jhong
authors.initials: GJ
authors.lastname: Huang
authors.affiliation: Natural Products Research Laboratories, UNC Eshelman School of Pharmacy, University of North Carolina, Chapel Hill, NC 27599, United States; Chinese Medicinal Research and Development Center, China Medical University Hospital, China Medical University, Taichung 404, Taiwan, ROC. Electronic address: khlee@unc.edu.
authors.forename: Kuo-Hsiung
authors.initials: KH
authors.lastname: Lee
authors.affiliation: School of Pharmacy, National Cheng Kung University Hospital, College of Medicine, National Cheng Kung University, Tainan 701, Taiwan, ROC; Department of Pharmacy, College of Pharmacy and Health Care, Tajen University, Pingtung 907, Taiwan, ROC.
authors.forename: Tian-Shung
authors.initials: TS
authors.lastname: Wu
mesh_terms: D000244:Adenosine Diphosphate / Q000494:pharmacology; D000818:Animals; D016718:Arachidonic Acid / Q000037:antagonists & inhibitors / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D005419:Flavonoids / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D051379:Mice; D015394:Molecular Structure; D031625:Morus / Q000737:chemistry*; D009569:Nitric Oxide / Q000037:antagonists & inhibitors* / Q000096:biosynthesis; D018517:Plant Roots / Q000737:chemistry*; D010972:Platelet Activating Factor / Q000037:antagonists & inhibitors / Q000494:pharmacology; D010974:Platelet Aggregation / Q000187:drug effects*; D000067996:RAW 264.7 Cells; D011817:Rabbits; D013329:Structure-Activity Relationship
chemical_list: D005419:Flavonoids; D010972:Platelet Activating Factor; D016718:Arachidonic Acid; D009569:Nitric Oxide; D000244:Adenosine Diphosphate
keywords: Anti-platelet aggregation; Morus australis; NO inhibitory activity; Pyranoflavonoid