title: Anti-cancer effect of lactic acid bacteria expressing antioxidant enzymes or IL-10 in a colorectal cancer mouse model.
abstract: The association between inflammatory bowel diseases and colorectal cancer is well documented. The genetic modification of lactic acid bacteria as a tool to increase the anti-inflammatory potential of these microorganisms has also been demonstrated. Thus the aim of the present work was to evaluate the anti-cancer potential of different genetically modified lactic acid bacteria (GM-LAB) producing antioxidant enzymes (catalase or superoxide dismutase) or the anti-inflammatory cytokine IL-10 (protein or DNA delivery) using a chemical induced colon cancer murine model. Dimethilhydrazine was used to induce colorectal cancer in mice. The animals received GM-LAB producing anti-oxidant enzymes, IL-10 or a mixture of different GM-LAB. Intestinal damage, enzyme activities and cytokines were evaluated and compared to the results obtained from mice that received the wild type strains from which derived the GM-LAB. All the GM-LAB assayed showed beneficial effects against colon cancer even though they exerted different mechanisms of action. The importance to select LAB with innate beneficial properties as the progenitor strain was demonstrated with the GM-LAB producing anti-oxidant enzymes. In addition, the best effects for the mixtures GM-LAB that combine different anti-inflammatory mechanism. Results indicate that mixtures of selected LAB and GM-LAB could be used as an adjunct treatment to decrease the inflammatory harmful environment associated to colorectal cancer, especially for patients with chronic intestinal inflammation who have an increased risk to develop colorectal cancer.
authors.affiliation: Centro de Referencia para Lactobacilos (CERELA-CONICET), San Miguel de Tucumán T4000ILC, Argentina.
authors.forename: Silvina
authors.initials: S
authors.lastname: Del Carmen
authors.affiliation: Centro de Referencia para Lactobacilos (CERELA-CONICET), San Miguel de Tucumán T4000ILC, Argentina.
authors.forename: Alejandra
authors.initials: A
authors.lastname: de Moreno de LeBlanc
authors.affiliation: Centro de Referencia para Lactobacilos (CERELA-CONICET), San Miguel de Tucumán T4000ILC, Argentina.
authors.forename: Romina
authors.initials: R
authors.lastname: Levit
authors.affiliation: Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, MG 31270-901, Brazil.
authors.forename: Vasco
authors.initials: V
authors.lastname: Azevedo
authors.affiliation: INRA, Commensal and Probiotics-Host Interactions Laboratory, UMR 1319 Micalis, F-78350 Jouy-en-Josas, France; Micalis Institute, INRA, AgroParisTech, Université Paris-Saclay, 78350 Jouy-en-Josas, France.
authors.forename: Philippe
authors.initials: P
authors.lastname: Langella
authors.affiliation: INRA, Commensal and Probiotics-Host Interactions Laboratory, UMR 1319 Micalis, F-78350 Jouy-en-Josas, France; Micalis Institute, INRA, AgroParisTech, Université Paris-Saclay, 78350 Jouy-en-Josas, France.
authors.forename: Luis G
authors.initials: LG
authors.lastname: Bermúdez-Humarán
authors.affiliation: Centro de Referencia para Lactobacilos (CERELA-CONICET), San Miguel de Tucumán T4000ILC, Argentina. Electronic address: leblanc@cerela.org.ar.
authors.forename: Jean Guy
authors.initials: JG
authors.lastname: LeBlanc
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000627:therapeutic use*; D000975:Antioxidants / Q000378:metabolism; D002374:Catalase / Q000235:genetics* / Q000378:metabolism; D015179:Colorectal Neoplasms / Q000139:chemically induced / Q000628:therapy*; D004195:Disease Models, Animal; D005260:Female; D006801:Humans; D018928:Immunity, Mucosal / Q000235:genetics; D015212:Inflammatory Bowel Diseases / Q000628:therapy*; D016753:Interleukin-10 / Q000235:genetics* / Q000627:therapeutic use; D019344:Lactic Acid / Q000378:metabolism; D013294:Lactococcus lactis / Q000502:physiology*; D051379:Mice; D008807:Mice, Inbred BALB C; D030781:Organisms, Genetically Modified; D013482:Superoxide Dismutase / Q000235:genetics* / Q000378:metabolism
chemical_list: D000970:Antineoplastic Agents; D000975:Antioxidants; D016753:Interleukin-10; D019344:Lactic Acid; D002374:Catalase; D013482:Superoxide Dismutase
keywords: Anti-inflammatory; Anti-oxidant; Cytokine; Genetic modification