title: Production of Bacillus amyloliquefaciens OG and its metabolites in renewable media: valorisation for biodiesel production and p-xylene decontamination.
abstract: Biosurfactants are important in many areas; however, costs impede large-scale production. This work aimed to develop a global sustainable strategy for the production of biosurfactants by a novel strain of Bacillus amyloliquefaciens. Initially, Bacillus sp. strain 0G was renamed B. amyloliquefaciens subsp. plantarum (syn. Bacillus velezensis) after analysis of the gyrA and gyrB DNA sequences. Growth in modified Landy's medium produced 3 main recoverable metabolites: surfactin, fengycin, and acetoin, which promote plant growth. Cultivation was studied in the presence of renewable carbon (as glycerol) and nitrogen (as arginine) sources. While diverse kinetics of acetoin production were observed in different media, similar yields (6-8 g·L-1) were obtained after 72 h of growth. Glycerol increased surfactin-specific production, while arginine increased the yields of surfactin and fengycin and increased biomass significantly. The specific production of fengycin increased ∼10 times, possibly due to a connecting pathway involving arginine and ornithine. Adding value to crude extracts and biomass, both were shown to be useful, respectively, for the removal of p-xylene from contaminated water and for biodiesel production, yielding ∼70 mg·g-1cells and glycerol, which could be recycled in novel media. This is the first study considering circular bioeconomy to lower the production costs of biosurfactants by valorisation of both microbial cells and their primary and secondary metabolites.
authors.affiliation: a Pontifical Catholic University of Campinas, Rodovia Dom Pedro I, km 136, Campinas, SP, 13086-900, Brazil.
authors.forename: Augusto
authors.initials: A
authors.lastname: Etchegaray
authors.affiliation: b ICV-Université Lille, EA 7394-ICV Institut Charles Viollette, F-59000 Lille, France.
authors.forename: François
authors.initials: F
authors.lastname: Coutte
authors.affiliation: b ICV-Université Lille, EA 7394-ICV Institut Charles Viollette, F-59000 Lille, France.
authors.forename: Gabrielle
authors.initials: G
authors.lastname: Chataigné
authors.affiliation: b ICV-Université Lille, EA 7394-ICV Institut Charles Viollette, F-59000 Lille, France.
authors.forename: Max
authors.initials: M
authors.lastname: Béchet
authors.affiliation: a Pontifical Catholic University of Campinas, Rodovia Dom Pedro I, km 136, Campinas, SP, 13086-900, Brazil.
authors.forename: Ramon H Z
authors.initials: RH
authors.lastname: Dos Santos
authors.affiliation: b ICV-Université Lille, EA 7394-ICV Institut Charles Viollette, F-59000 Lille, France.
authors.forename: Valérie
authors.initials: V
authors.lastname: Leclère
authors.affiliation: b ICV-Université Lille, EA 7394-ICV Institut Charles Viollette, F-59000 Lille, France.
authors.forename: Philippe
authors.initials: P
authors.lastname: Jacques
mesh_terms: D000069977:Bacillus amyloliquefaciens / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D001673:Biodegradation, Environmental; D056804:Biofuels / Q000032:analysis*; D018533:Biomass; D003470:Culture Media / Q000378:metabolism*; D005990:Glycerol / Q000378:metabolism; D055666:Lipopeptides / Q000378:metabolism; D010456:Peptides, Cyclic / Q000378:metabolism; D014874:Water Pollutants, Chemical / Q000378:metabolism*; D014992:Xylenes / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D056804:Biofuels; D003470:Culture Media; D055666:Lipopeptides; D010456:Peptides, Cyclic; D014874:Water Pollutants, Chemical; D014992:Xylenes; C049972:fengycin; C009365:surfactin peptide; C031286:4-xylene; D005990:Glycerol
keywords: Bacillus sp.; biosurfactants; bioéconomie circulaire; circular bioeconomy; durabilité; décontamination environnementale; environmental decontamination; lipopeptides; sustainability