title: Ginsenoside Re Inhibits Osteoclast Differentiation in Mouse Bone Marrow-Derived Macrophages and Zebrafish Scale Model.
abstract: Ginsenosides, which are the active materials of ginseng, have biological functions that include anti-osteoporotic effects. Aqueous ginseng extract inhibits osteoclast differentiation induced by receptor activator of NF-κB ligand (RANKL). Aqueous ginseng extract produces chromatography peaks characteristic of ginsenosides. Among these peaks, ginsenoside Re is a major component. However, the preventive effects of ginsenoside Re against osteoclast differentiation are not known. We studied the effect of ginsenoside Re on osteoclast differentiation, RANKL-induced tartrate-resistant acid phosphatase (TRAP) activity, and formation of multinucleated osteoclastsin vitro. Ginsenoside Re hampered osteoclast differentiation in a dose-dependent manner.In anin vivo zebrafish model, aqueous ginseng extract and ginsenoside Re had anti-osteoclastogenesis effects. These findings suggest that both aqueous ginseng extract and ginsenoside Re prevent bone resorption by inhibiting osteoclast differentiation. Ginsenoside Re could be important for promoting bone health.
authors.affiliation: World Class Institute (WCI), and Chemical biology Research Center, Korean Research Institute of Bioscience and Biotechnology (KRIBB), Cheongju 28116, Korea.|Department of Biochemistry, College of Nature science, Chungnam National University, Daejeon 34134, Korea.
authors.forename: Chan-Mi
authors.initials: CM
authors.lastname: Park
authors.affiliation: World Class Institute (WCI), and Chemical biology Research Center, Korean Research Institute of Bioscience and Biotechnology (KRIBB), Cheongju 28116, Korea.|Biomolecular Science, University of Science and Technology, Daejeon 34113, Korea.
authors.forename: Hye-Min
authors.initials: HM
authors.lastname: Kim
authors.affiliation: World Class Institute (WCI), and Chemical biology Research Center, Korean Research Institute of Bioscience and Biotechnology (KRIBB), Cheongju 28116, Korea.|Biomolecular Science, University of Science and Technology, Daejeon 34113, Korea.
authors.forename: Dong Hyun
authors.initials: DH
authors.lastname: Kim
authors.affiliation: World Class Institute (WCI), and Chemical biology Research Center, Korean Research Institute of Bioscience and Biotechnology (KRIBB), Cheongju 28116, Korea.|Biomolecular Science, University of Science and Technology, Daejeon 34113, Korea.
authors.forename: Ho-Jin
authors.initials: HJ
authors.lastname: Han
authors.affiliation: World Class Institute (WCI), and Chemical biology Research Center, Korean Research Institute of Bioscience and Biotechnology (KRIBB), Cheongju 28116, Korea.
authors.forename: Haneul
authors.initials: H
authors.lastname: Noh
authors.affiliation: World Class Institute (WCI), and Chemical biology Research Center, Korean Research Institute of Bioscience and Biotechnology (KRIBB), Cheongju 28116, Korea.|Biomolecular Science, University of Science and Technology, Daejeon 34113, Korea.
authors.forename: Jae-Hyuk
authors.initials: JH
authors.lastname: Jang
authors.affiliation: Clinical Trial Center for Functional Foods (CTCF2), Chonbuk National University Hospital, Jeonju 54907, Korea.
authors.forename: Soo-Hyun
authors.initials: SH
authors.lastname: Park
authors.affiliation: Clinical Trial Center for Functional Foods (CTCF2), Chonbuk National University Hospital, Jeonju 54907, Korea.
authors.forename: Han-Jung
authors.initials: HJ
authors.lastname: Chae
authors.affiliation: Clinical Trial Center for Functional Foods (CTCF2), Chonbuk National University Hospital, Jeonju 54907, Korea.
authors.forename: Soo-Wan
authors.initials: SW
authors.lastname: Chae
authors.affiliation: Center of Magnetic Resonance Research, Korea Basic Science Institute, Cheongju 28119, Korea.
authors.forename: Eun Kyoung
authors.initials: EK
authors.lastname: Ryu
authors.affiliation: World Class Institute (WCI), and Chemical biology Research Center, Korean Research Institute of Bioscience and Biotechnology (KRIBB), Cheongju 28116, Korea.
authors.forename: Sangku
authors.initials: S
authors.lastname: Lee
authors.affiliation: World Class Institute (WCI), and Chemical biology Research Center, Korean Research Institute of Bioscience and Biotechnology (KRIBB), Cheongju 28116, Korea.
authors.forename: Kangdong
authors.initials: K
authors.lastname: Liu
authors.affiliation: World Class Institute (WCI), and Chemical biology Research Center, Korean Research Institute of Bioscience and Biotechnology (KRIBB), Cheongju 28116, Korea.
authors.forename: Haidan
authors.initials: H
authors.lastname: Liu
authors.affiliation: World Class Institute (WCI), and Chemical biology Research Center, Korean Research Institute of Bioscience and Biotechnology (KRIBB), Cheongju 28116, Korea.|Biomolecular Science, University of Science and Technology, Daejeon 34113, Korea.
authors.forename: Jong-Seog
authors.initials: JS
authors.lastname: Ahn
authors.affiliation: Department of Medicinal Crop Research Institute, National Institute of Horticultural & Herbal Science, Rural Development Administration, Eumseong 27709, Korea.
authors.forename: Young Ock
authors.initials: YO
authors.lastname: Kim
authors.affiliation: World Class Institute (WCI), and Chemical biology Research Center, Korean Research Institute of Bioscience and Biotechnology (KRIBB), Cheongju 28116, Korea.|Biomolecular Science, University of Science and Technology, Daejeon 34113, Korea.
authors.forename: Bo-Yeon
authors.initials: BY
authors.lastname: Kim
authors.affiliation: World Class Institute (WCI), and Chemical biology Research Center, Korean Research Institute of Bioscience and Biotechnology (KRIBB), Cheongju 28116, Korea.|Biomolecular Science, University of Science and Technology, Daejeon 34113, Korea.
authors.forename: Nak-Kyun
authors.initials: NK
authors.lastname: Soung
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology / Q000187:drug effects*; D002454:Cell Differentiation / Q000187:drug effects; D036145:Ginsenosides / Q000494:pharmacology*; D008264:Macrophages / Q000166:cytology / Q000187:drug effects*; D051379:Mice; D010010:Osteoclasts / Q000166:cytology / Q000187:drug effects*; D015027:Zebrafish
chemical_list: D036145:Ginsenosides; C049864:ginsenoside Re
keywords: RANKL; ginsenoside Re; osteoclasts; zebrafish