title: Blautia massiliensis sp. nov., isolated from a fresh human fecal sample and emended description of the genus Blautia.
abstract: The strain GD9Tis the type strain of the newly proposed species Blautia massiliensis sp. nov., belonging to the family Lachnospiraceae. It was isolated from a fresh stool sample collected from a healthy human using the culturomics strategy. Cells are Gram-negative rods, oxygen intolerant, non-motile and non-spore forming. The 16S rRNA gene sequencing showed that strain GD9Twas closely related to Blautia luti, with a 97.8% sequence similarity. Major fatty acids were C14:0 (19.8%) and C16:0 (53.2%). Strain GD9Texhibits a genome of 3,717,339 bp that contains 3,346 protein-coding genes and 81 RNAs genes including 63 tRNAs. The features of this organism are described here, with its complete genome sequence and annotation. Compared with other Blautia species which are Gram positive, the strain was Gram negative justifying an emended description of the genus Blautia.
authors.affiliation: URMITE UM63, CNRS7278, IRD198, INSERM1095, Faculté de Médecine, Aix Marseille Université, 27 Boulevard Jean Moulin, 13385, Marseille Cedex 5, France; Pôle des Maladies Infectieuses, Hôpital La Timone, Assistance Publique-Hôpitaux de Marseille, Marseille, France.
authors.forename: Guillaume A
authors.initials: GA
authors.lastname: Durand
authors.affiliation: URMITE UM63, CNRS7278, IRD198, INSERM1095, Faculté de Médecine, Aix Marseille Université, 27 Boulevard Jean Moulin, 13385, Marseille Cedex 5, France.
authors.forename: Thao
authors.initials: T
authors.lastname: Pham
authors.affiliation: URMITE UM63, CNRS7278, IRD198, INSERM1095, Faculté de Médecine, Aix Marseille Université, 27 Boulevard Jean Moulin, 13385, Marseille Cedex 5, France.
authors.forename: Sokhna
authors.initials: S
authors.lastname: Ndongo
authors.affiliation: URMITE UM63, CNRS7278, IRD198, INSERM1095, Faculté de Médecine, Aix Marseille Université, 27 Boulevard Jean Moulin, 13385, Marseille Cedex 5, France.
authors.forename: Sory Ibrahima
authors.initials: SI
authors.lastname: Traore
authors.affiliation: URMITE UM63, CNRS7278, IRD198, INSERM1095, Faculté de Médecine, Aix Marseille Université, 27 Boulevard Jean Moulin, 13385, Marseille Cedex 5, France; Pôle des Maladies Infectieuses, Hôpital La Timone, Assistance Publique-Hôpitaux de Marseille, Marseille, France.
authors.forename: Grégory
authors.initials: G
authors.lastname: Dubourg
authors.affiliation: URMITE UM63, CNRS7278, IRD198, INSERM1095, Faculté de Médecine, Aix Marseille Université, 27 Boulevard Jean Moulin, 13385, Marseille Cedex 5, France; Pôle des Maladies Infectieuses, Hôpital La Timone, Assistance Publique-Hôpitaux de Marseille, Marseille, France.
authors.forename: Jean-Christophe
authors.initials: JC
authors.lastname: Lagier
authors.affiliation: URMITE UM63, CNRS7278, IRD198, INSERM1095, Faculté de Médecine, Aix Marseille Université, 27 Boulevard Jean Moulin, 13385, Marseille Cedex 5, France.
authors.forename: Caroline
authors.initials: C
authors.lastname: Michelle
authors.affiliation: URMITE UM63, CNRS7278, IRD198, INSERM1095, Faculté de Médecine, Aix Marseille Université, 27 Boulevard Jean Moulin, 13385, Marseille Cedex 5, France.
authors.forename: Nicholas
authors.initials: N
authors.lastname: Armstrong
authors.affiliation: URMITE UM63, CNRS7278, IRD198, INSERM1095, Faculté de Médecine, Aix Marseille Université, 27 Boulevard Jean Moulin, 13385, Marseille Cedex 5, France; Pôle des Maladies Infectieuses, Hôpital La Timone, Assistance Publique-Hôpitaux de Marseille, Marseille, France.
authors.forename: Pierre-Edouard
authors.initials: PE
authors.lastname: Fournier
authors.affiliation: URMITE UM63, CNRS7278, IRD198, INSERM1095, Faculté de Médecine, Aix Marseille Université, 27 Boulevard Jean Moulin, 13385, Marseille Cedex 5, France; Pôle des Maladies Infectieuses, Hôpital La Timone, Assistance Publique-Hôpitaux de Marseille, Marseille, France; Special Infectious Agents Unit, King Fahd Medical Research Center, King Abdulaziz University, Jeddah, Saudi Arabia.
authors.forename: Didier
authors.initials: D
authors.lastname: Raoult
authors.affiliation: URMITE UM63, CNRS7278, IRD198, INSERM1095, Faculté de Médecine, Aix Marseille Université, 27 Boulevard Jean Moulin, 13385, Marseille Cedex 5, France; Pôle des Maladies Infectieuses, Hôpital La Timone, Assistance Publique-Hôpitaux de Marseille, Marseille, France. Electronic address: matthieumillion@gmail.com.
authors.forename: Matthieu
authors.initials: M
authors.lastname: Million
mesh_terms: D000328:Adult; D000068016:Eubacteriales / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D005243:Feces / Q000382:microbiology; D000069196:Gastrointestinal Microbiome; D016680:Genome, Bacterial / Q000235:genetics*; D006801:Humans; D008297:Male; D054892:Metagenome; D058977:Molecular Sequence Annotation; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000737:chemistry / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S
keywords: Anaerobe; Blautia massiliensis sp. nov.; Culturomics; Gut microbiota; Taxono-genomics