title: Cyclosporine A inhibits TGF-β2-induced myofibroblasts of primary cultured human pterygium fibroblasts.
abstract: Cyclosporine A (CsA), an immunomodulatory drug, and is increasingly used to treat moderate dry eye syndrome and ocular surface inflammation. However, any inhibitory effect on differentiation of fibroblasts to myofibroblasts remains unclear. Here, we show that the inhibitory effect of CsA on transforming growth factor-beta2 (TGF-β2)-induced myofibroblasts in primary cultured human pterygium fibroblasts. CsA significantly decreased mRNA and protein expression of myofibroblast-related markers including α-SMA, laminin, and fibronectin. These findings were supported by the results from immunofluorescence staining. Taken together, these results indicate the therapeutic potential of CsA against pterygium progression. Further studies are necessary to elucidate the precise intracellular signal mechanism responsible for CsA-induced downregulation of myofibroblast markers in pterygium fibroblasts.
authors.affiliation: Cheil Eye Research Institute, Cheil Eye Hospital, 1 Ayang-ro, Dong-gu, Daegu, 701-820, Republic of Korea.
authors.forename: Sang Il
authors.initials: SI
authors.lastname: Gum
authors.affiliation: Cheil Eye Research Institute, Cheil Eye Hospital, 1 Ayang-ro, Dong-gu, Daegu, 701-820, Republic of Korea; Korean Medicine (KM)-Application Centre, Korea Institute of Oriental Medicine (KIOM), 70, Chemdan-ro, Dong-gu, Daegu, 701-300, Republic of Korea.
authors.forename: Yeoun-Hee
authors.initials: YH
authors.lastname: Kim
authors.affiliation: Developmental Biology Laboratory, Department of Biology, College of Natural Sciences, Kyungpook National University, Daegu, 702-701, Republic of Korea.
authors.forename: Jae-Chang
authors.initials: JC
authors.lastname: Jung
authors.affiliation: Cheil Eye Research Institute, Cheil Eye Hospital, 1 Ayang-ro, Dong-gu, Daegu, 701-820, Republic of Korea.
authors.forename: Im Gyu
authors.initials: IG
authors.lastname: Kim
authors.affiliation: Cheil Eye Research Institute, Cheil Eye Hospital, 1 Ayang-ro, Dong-gu, Daegu, 701-820, Republic of Korea.
authors.forename: Jun Seok
authors.initials: JS
authors.lastname: Lee
authors.affiliation: Cheil Eye Research Institute, Cheil Eye Hospital, 1 Ayang-ro, Dong-gu, Daegu, 701-820, Republic of Korea.
authors.forename: Kyoo Won
authors.initials: KW
authors.lastname: Lee
authors.affiliation: Cheil Eye Research Institute, Cheil Eye Hospital, 1 Ayang-ro, Dong-gu, Daegu, 701-820, Republic of Korea. Electronic address: eyepark9@naver.com.
authors.forename: Young Jeung
authors.initials: YJ
authors.lastname: Park
mesh_terms: D000199:Actins / Q000378:metabolism; D002454:Cell Differentiation; D002478:Cells, Cultured; D016572:Cyclosporine / Q000494:pharmacology*; D005260:Female; D005347:Fibroblasts / Q000378:metabolism*; D005353:Fibronectins / Q000378:metabolism; D006801:Humans; D007166:Immunosuppressive Agents / Q000494:pharmacology; D007249:Inflammation; D007797:Laminin / Q000378:metabolism; D008297:Male; D008856:Microscopy, Fluorescence; D009130:Muscle, Smooth / Q000378:metabolism; D058628:Myofibroblasts / Q000378:metabolism; D009841:Oligonucleotides / Q000737:chemistry; D011625:Pterygium / Q000188:drug therapy* / Q000378:metabolism* / Q000601:surgery; D015398:Signal Transduction; D012984:Software; D053781:Transforming Growth Factor beta2 / Q000494:pharmacology
chemical_list: D000199:Actins; D005353:Fibronectins; D007166:Immunosuppressive Agents; D007797:Laminin; D009841:Oligonucleotides; C509041:TGFB2 protein, human; D053781:Transforming Growth Factor beta2; D016572:Cyclosporine
keywords: Cyclosporine A; Fibronectin; Laminin; Myofibroblasts; Pterygium fibroblasts; TGF-β2; α-SMA