title: Advances in capillary electrophoresis and the implications for drug discovery.
abstract: Many screening platforms are prone to assay interferences that can be avoided by directly measuring the target or enzymatic product. Capillary electrophoresis (CE) and microchip electrophoresis (MCE) have been applied in a variety of formats to drug discovery. CE provides direct detection of the product allowing for the identification of some forms of assay interference. The high efficiency, rapid separations, and low volume requirements make CE amenable to drug discovery. Areas covered: This article describes advances in capillary electrophoresis throughput, sample introduction, and target assays as they pertain to drug discovery and screening. Instrumental advances discussed include integrated droplet microfluidics platforms and multiplexed arrays. Applications of CE to assays of diverse drug discovery targets, including enzymes and affinity interactions are also described. Expert opinion: Current screening with CE does not fully take advantage of the throughputs or low sample volumes possible with CE and is most suitable as a secondary screening method or for screens that are inaccessible with more common platforms. With further development, droplet microfluidics coupled to MCE could take advantage of the low sample requirements by performing assays on the nanoliter scale at high throughput.
authors.affiliation: a Department of Chemistry , University of Michigan , Ann Arbor , MI , United States.
authors.forename: Claire M
authors.initials: CM
authors.lastname: Ouimet
authors.affiliation: b Department of Pharmacology , University of Michigan , Ann Arbor , MI , United States.
authors.forename: Cara I
authors.initials: CI
authors.lastname: D'amico
authors.affiliation: a Department of Chemistry , University of Michigan , Ann Arbor , MI , United States.|b Department of Pharmacology , University of Michigan , Ann Arbor , MI , United States.
authors.forename: Robert T
authors.initials: RT
authors.lastname: Kennedy
mesh_terms: D055808:Drug Discovery / Q000379:methods*; D019075:Electrophoresis, Capillary / Q000379:methods*; D046209:Electrophoresis, Microchip / Q000379:methods*; D057166:High-Throughput Screening Assays / Q000379:methods; D006801:Humans; D046210:Microfluidic Analytical Techniques / Q000379:methods; D058990:Molecular Targeted Therapy
keywords: Capillary electrophoresis; microchip electrophoresis; microfluidics; screening