title: STAT3 stimulates adipogenic stem cell proliferation and cooperates with HMGA2 during the early stage of differentiation to promote adipogenesis.
abstract: Signal transducer and activator of transcription 3 (STAT3) is abundantly expressed in the adipose tissue of obese mice and humans, but the role of STAT3 in adipogenesis is still not fully understood. In the present study, we discovered an activation of STAT3 during the early differentiation stage of mouse 3T3-L1 preadipocytes. Stat3 knockdown using siRNA blocked cell cycle progression of both preadipoctes and early differentiating cells. Moreover, accumulation of lipid droplets was inhibited by Stat3 knockdown. Importantly, in the nucleus of early differentiating cells, we demonstrated that STAT3 protein co-localized with high-mobility-group protein AT-hook 2 (HMGA2), which was reported to promote adipogenesis in a previous study. Taken together, our data indicate that STAT3 and HMGA2 cooperatively promote adipogenesis which highlight a more detail understanding of STAT3 related transcription factor network during adipogenesis.
authors.affiliation: Runliang Diabetes Laboratory, Diabetes Research Center, Zhejiang Provincial Key Laboratory of Pathophysiology, School of Medicine, Ningbo University, Ningbo, 315211, China. Electronic address: 1511101190@nbu.edu.cn.
authors.forename: Yanyan
authors.initials: Y
authors.lastname: Yuan
authors.affiliation: Runliang Diabetes Laboratory, Diabetes Research Center, Zhejiang Provincial Key Laboratory of Pathophysiology, School of Medicine, Ningbo University, Ningbo, 315211, China. Electronic address: xiyang@nbu.edu.cn.
authors.forename: Yang
authors.initials: Y
authors.lastname: Xi
authors.affiliation: Runliang Diabetes Laboratory, Diabetes Research Center, Zhejiang Provincial Key Laboratory of Pathophysiology, School of Medicine, Ningbo University, Ningbo, 315211, China. Electronic address: 1047395416@qq.com.
authors.forename: Jiayi
authors.initials: J
authors.lastname: Chen
authors.affiliation: Runliang Diabetes Laboratory, Diabetes Research Center, Zhejiang Provincial Key Laboratory of Pathophysiology, School of Medicine, Ningbo University, Ningbo, 315211, China. Electronic address: 1119300591@qq.com.
authors.forename: Pan
authors.initials: P
authors.lastname: Zhu
authors.affiliation: Runliang Diabetes Laboratory, Diabetes Research Center, Zhejiang Provincial Key Laboratory of Pathophysiology, School of Medicine, Ningbo University, Ningbo, 315211, China. Electronic address: 924528232@qq.com.
authors.forename: Jinyu
authors.initials: J
authors.lastname: Kang
authors.affiliation: Runliang Diabetes Laboratory, Diabetes Research Center, Zhejiang Provincial Key Laboratory of Pathophysiology, School of Medicine, Ningbo University, Ningbo, 315211, China. Electronic address: zouzuquan@nbu.edu.cn.
authors.forename: Zuquan
authors.initials: Z
authors.lastname: Zou
authors.affiliation: Runliang Diabetes Laboratory, Diabetes Research Center, Zhejiang Provincial Key Laboratory of Pathophysiology, School of Medicine, Ningbo University, Ningbo, 315211, China. Electronic address: wangfuyan@nbu.edu.cn.
authors.forename: Fuyan
authors.initials: F
authors.lastname: Wang
authors.affiliation: Runliang Diabetes Laboratory, Diabetes Research Center, Zhejiang Provincial Key Laboratory of Pathophysiology, School of Medicine, Ningbo University, Ningbo, 315211, China. Electronic address: bushizhong@nbu.edu.cn.
authors.forename: Shizhong
authors.initials: S
authors.lastname: Bu
mesh_terms: D016475:3T3 Cells; D041721:3T3-L1 Cells; D017667:Adipocytes / Q000166:cytology*; D050156:Adipogenesis; D000273:Adipose Tissue / Q000166:cytology; D000818:Animals; D002454:Cell Differentiation; D049109:Cell Proliferation; D005786:Gene Expression Regulation; D025743:HMGA2 Protein / Q000378:metabolism*; D008055:Lipids / Q000737:chemistry; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D034741:RNA, Small Interfering / Q000378:metabolism; D050796:STAT3 Transcription Factor / Q000378:metabolism*; D013234:Stem Cells / Q000166:cytology*
chemical_list: D025743:HMGA2 Protein; D008055:Lipids; D034741:RNA, Small Interfering; D050796:STAT3 Transcription Factor; C494087:Stat3 protein, mouse
keywords: Adipogenesis; HMGA2; Proliferation; STAT3