title: Nonwoven Carboxylated Agarose-Based Fiber Meshes with Antimicrobial Properties.
abstract: Hydrogel forming polysaccharides, such as the seaweed derived agarose, are well suited for wound dressing applications as they have excellent cell and soft tissue compatibility. For wound dressings, fibrous structure is desirable as the high surface area can favor adsorption of wound exudate and promote drug delivery. Although electrospinning offers a straightforward means to produce nonwoven fibrous polymeric structures, processing agarose and its derivatives into fibers through electrospinning is challenging as it has limited solubility in solvents other than water. In this study we describe the processing of carboxylated agarose (CA) fibers with antibacterial properties by electrospinning from a solution of the ionic liquid (IL) 1-butyl-3-methylimidazolium chloride ([Bmim]+Cl-) possessing antimicrobial properties. The extent of carboxylation was found to impact fiber diameter, mesh elastic modulus, fiber swelling, and the loading and release of IL. IL-bearing CA fibers inhibited the growth of Staphylococcus aureus and Pseudomonas aeruginosa, bacteria commonly found in wound exudate. In sum, nonwoven CA fibers processed from IL are promising as biomaterials for wound dressing applications.
authors.affiliation: Future Industries Institute, University of South Australia , Mawson Lakes 5095, South Australia, Australia.|Institute for Macromolecular Chemistry, University of Freiburg , 79104, Freiburg, Germany.
authors.forename: Aurelien
authors.initials: A
authors.lastname: Forget
authors.affiliation: Institute for Macromolecular Chemistry, University of Freiburg , 79104, Freiburg, Germany.|Helmholtz Virtual Institute on Multifunctional Biomaterials for Medicine, Kantstraße 5514513, Teltow, Germany.
authors.forename: Neha
authors.initials: N
authors.lastname: Arya
authors.affiliation: Institute for Macromolecular Chemistry, University of Freiburg , 79104, Freiburg, Germany.
authors.forename: Rotsiniaina
authors.initials: R
authors.lastname: Randriantsilefisoa
authors.affiliation: Institute for Macromolecular Chemistry, University of Freiburg , 79104, Freiburg, Germany.
authors.forename: Florian
authors.initials: F
authors.lastname: Miessmer
authors.affiliation: Department of Environmental Health Science, Universitätklinikum Freiburg , Freiburg 79106, Germany.
authors.forename: Marion
authors.initials: M
authors.lastname: Buck
authors.affiliation: Institute for Macromolecular Chemistry, University of Freiburg , 79104, Freiburg, Germany.
authors.forename: Vincent
authors.initials: V
authors.lastname: Ahmadi
authors.affiliation: Department of Environmental Health Science, Universitätklinikum Freiburg , Freiburg 79106, Germany.
authors.forename: Daniel
authors.initials: D
authors.lastname: Jonas
authors.affiliation: School of Pharmacy and Medical Sciences, University of South Australia , Mawson Lakes 5095, South Australia, Australia.
authors.forename: Anton
authors.initials: A
authors.lastname: Blencowe
authors.affiliation: Institute for Macromolecular Chemistry, University of Freiburg , 79104, Freiburg, Germany.|Helmholtz Virtual Institute on Multifunctional Biomaterials for Medicine, Kantstraße 5514513, Teltow, Germany.|BIOSS-Centre for Biological Signaling Studies, University of Freiburg , 79104, Freiburg, Germany.
authors.forename: V Prasad
authors.initials: VP
authors.lastname: Shastri
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology*; D002264:Carboxylic Acids / Q000737:chemistry*; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000737:chemistry*; D008826:Microbial Sensitivity Tests; D057139:Nanofibers / Q000737:chemistry*; D011550:Pseudomonas aeruginosa / Q000187:drug effects*; D012685:Sepharose / Q000737:chemistry*; D013211:Staphylococcus aureus / Q000187:drug effects*
chemical_list: D000900:Anti-Bacterial Agents; D002264:Carboxylic Acids; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D012685:Sepharose