title: Impact of a potential glycosylation site at neuraminidase amino acid 264 of influenza A/H9N2 virus.
abstract: To determine the role of the potential glycosylation site NA264N, which has been shown to be prevalent in recent Chinese H9N2 isolates, four reverse genetic viruses, rgWS1-NA264N, rgWS1-NA264H, rgBJ-NA264H and rgBJ-NA264N, were rescued. Growth kinetics showed that viruses with NA264H grew faster than viruses with NA264N. Mouse studies revealed that rgBJ-NA264H replicated to a significantly higher titer than rgBJ-NA264N at 3dpi. Notably, in contact chickens, rgBJ-NA264H and rgWS1-NA264H shed significantly more virus than rgBJ-NA264N at 6dpi from the larynx and rgWS1-NA264N at 4dpi from the cloaca, respectively. The present study demonstrates that NA264N affects viral replication of H9N2.
authors.affiliation: Ministry of Education Key Laboratory for Avian Preventive Medicine, and Key Laboratory of Jiangsu Preventive Veterinary Medicine, Yangzhou University, Yangzhou, Jiangsu, PR China; Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, Jiangsu, PR China.
authors.forename: Hongxia
authors.initials: H
authors.lastname: Shao
authors.affiliation: Ministry of Education Key Laboratory for Avian Preventive Medicine, and Key Laboratory of Jiangsu Preventive Veterinary Medicine, Yangzhou University, Yangzhou, Jiangsu, PR China; Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, Jiangsu, PR China.
authors.forename: Xiaoxiang
authors.initials: X
authors.lastname: Zhou
authors.affiliation: Ministry of Education Key Laboratory for Avian Preventive Medicine, and Key Laboratory of Jiangsu Preventive Veterinary Medicine, Yangzhou University, Yangzhou, Jiangsu, PR China; Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, Jiangsu, PR China.
authors.forename: Zhonglei
authors.initials: Z
authors.lastname: Fan
authors.affiliation: Ministry of Education Key Laboratory for Avian Preventive Medicine, and Key Laboratory of Jiangsu Preventive Veterinary Medicine, Yangzhou University, Yangzhou, Jiangsu, PR China; Poultry Diagnostic and Research Center, University of Georgia, Athens, GA, USA.
authors.forename: Zhimin
authors.initials: Z
authors.lastname: Wan
authors.affiliation: Ministry of Education Key Laboratory for Avian Preventive Medicine, and Key Laboratory of Jiangsu Preventive Veterinary Medicine, Yangzhou University, Yangzhou, Jiangsu, PR China; Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, Jiangsu, PR China.
authors.forename: Kun
authors.initials: K
authors.lastname: Qian
authors.affiliation: Poultry Diagnostic and Research Center, University of Georgia, Athens, GA, USA.
authors.forename: Daniel
authors.initials: D
authors.lastname: Perez
authors.affiliation: Ministry of Education Key Laboratory for Avian Preventive Medicine, and Key Laboratory of Jiangsu Preventive Veterinary Medicine, Yangzhou University, Yangzhou, Jiangsu, PR China; Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, Jiangsu, PR China. Electronic address: aijian@yzu.edu.cn.
authors.forename: Aijian
authors.initials: A
authors.lastname: Qin
authors.affiliation: Ministry of Education Key Laboratory for Avian Preventive Medicine, and Key Laboratory of Jiangsu Preventive Veterinary Medicine, Yangzhou University, Yangzhou, Jiangsu, PR China; Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, Jiangsu, PR China. Electronic address: jqye@yzu.edu.cn.
authors.forename: Jianqiang
authors.initials: J
authors.lastname: Ye
mesh_terms: D000818:Animals; D002645:Chickens / Q000821:virology*; D006031:Glycosylation; D053127:Influenza A Virus, H9N2 Subtype / Q000201:enzymology* / Q000235:genetics / Q000472:pathogenicity / Q000502:physiology; D005585:Influenza in Birds / Q000821:virology*; D051379:Mice; D008958:Models, Molecular; D009439:Neuraminidase / Q000235:genetics / Q000378:metabolism*; D011201:Poultry Diseases / Q000821:virology*; D014779:Virus Replication
chemical_list: D009439:Neuraminidase
keywords: H9N2; NA264N; Pathogenesis; Viral replication; Virus rescuing