title: Detection of Transcriptionally Active Mycotoxin Gene Clusters: DNA Microarray.
abstract: Various bioanalytical tools including DNA microarrays are frequently used to map global transcriptional changes in mycotoxin producer filamentous fungi. This effective hybridization-based transcriptomics technology helps researchers to identify genes of secondary metabolite gene clusters and record concomitant gene expression changes in these clusters initiated by versatile environmental conditions and/or gene deletions. Such transcriptional data are of great value when future mycotoxin control technologies are considered and elaborated. Giving the readers insights into RNA extraction and DNA microarray hybridization steps routinely used in our laboratories and also into the normalization and evaluation of primary gene expression data, we would like to contribute to the interlaboratory standardization of DNA microarray based transcriptomics studies being carried out in many laboratories worldwide in this important field of fungal biology.
authors.affiliation: Faculty of Science and Technology, Department of Biotechnology and Microbiology,, University of Debrecen, Egyetem tér 1., H-4032, Debrecen, Hungary.
authors.forename: Tamás
authors.initials: T
authors.lastname: Emri
authors.affiliation: Kromat Ltd., Péterhegyi út 98., H-1112, Budapest, Hungary.
authors.forename: Anna
authors.initials: A
authors.lastname: Zalka
authors.affiliation: Faculty of Science and Technology, Department of Biotechnology and Microbiology,, University of Debrecen, Egyetem tér 1., H-4032, Debrecen, Hungary. pocsi.istvan@science.unideb.hu.
authors.forename: István
authors.initials: I
authors.lastname: Pócsi
mesh_terms: D019295:Computational Biology / Q000379:methods; D005658:Fungi / Q000235:genetics; D020869:Gene Expression Profiling; D015966:Gene Expression Regulation, Fungal; D005810:Multigene Family; D009183:Mycotoxins / Q000235:genetics*; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods; D014158:Transcription, Genetic; D059467:Transcriptome
chemical_list: D009183:Mycotoxins
keywords: Acidic guanidinium thiocyanate-phenol reagent; Culture conditions; DNA microarray; Environmental stress; Gene clusters; Gene deletion mutants; Mycotoxins; RNA extraction; Secondary metabolism; Transcriptomics