title: Spontaneous mouse mammary tumor cell lysates induce IgG production in spleen mononuclear cells of healthy and tumor-bearing mice.
abstract: BACKGROUND
  We hypothesized that Tumor cell lysate (TCL) prepared from spontaneous mouse mammary tumor (SMMT) may elicit IgG production by spleen mononuclear cells (SMCs) in ex vivo.
  
  
  METHODS
  The SMCs from healthy mice (HM, n = 6) and four-week SMMT-bearing mice (TBM, n = 6) was cultured in presence of TCL and mitogen for 42 hr at 37°C, separately. Serum and SMCs culture supernatant levels of IFNγ, IL-4, and total IgG were measured using special ELISA kits.
  
  
  RESULTS
  Serum IgG level of TBM was significantly higher than that of HM group (P = 0.019), while serum IFNγ and IL-4 did not differ between two groups (P > 0.05). Mitogen significantly induced ex vivo production of both IFNγ (P = 0.013) and IL-4 (P = 0.015) by SMCs from HM group, and only IL-4 (P = 0.049) by SMCs from TBM group. In contrast, TCL increased ex vivo production of IgG by SMCs from both HM (P = 0.034) and TBM (P = 0.016) groups. The ex vivo IgG revealed a moderate positive correlation with tumor size (r = 0.578, P = 0.422).
  
  
  CONCLUSIONS
  It seems that TCL prepared from SMMT are potent inducers of IgG production. This may propose TCL as a potential tool for monitoring of humoral immunity in animal models.
authors.affiliation: a Department of Immunology, School of Medicine , Shahid Beheshti University of Medical Sciences , Tehran , Iran.
authors.forename: Ahad
authors.initials: A
authors.lastname: Khalilnezhad
authors.affiliation: a Department of Immunology, School of Medicine , Shahid Beheshti University of Medical Sciences , Tehran , Iran.
authors.forename: Elham
authors.initials: E
authors.lastname: Mahmoudian
authors.affiliation: a Department of Immunology, School of Medicine , Shahid Beheshti University of Medical Sciences , Tehran , Iran.
authors.forename: Nariman
authors.initials: N
authors.lastname: Mosaffa
authors.affiliation: b Department of Pathology, School of Medicine , Shahid Beheshti University of Medical Sciences , Tehran , Iran.
authors.forename: Jaleh
authors.initials: J
authors.lastname: Mohsenifar
authors.affiliation: a Department of Immunology, School of Medicine , Shahid Beheshti University of Medical Sciences , Tehran , Iran.
authors.forename: Davar
authors.initials: D
authors.lastname: Amani
mesh_terms: D000818:Animals; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D007074:Immunoglobulin G / Q000096:biosynthesis* / Q000097:blood / Q000276:immunology; D007371:Interferon-gamma / Q000097:blood; D015847:Interleukin-4 / Q000097:blood; D015674:Mammary Neoplasms, Animal / Q000276:immunology* / Q000473:pathology; D051379:Mice; D008807:Mice, Inbred BALB C; D013154:Spleen / Q000166:cytology* / Q000276:immunology*; D014407:Tumor Cells, Cultured
chemical_list: D007074:Immunoglobulin G; D015847:Interleukin-4; D007371:Interferon-gamma
keywords: humoral immunity; immunoglobulin; tumor cell lysate