title: Trace isotope analysis of Ricinus communis seed core for provenance determination by laser ablation-ICP-MS.
abstract: The castor bean plant, Ricinus communis, grows wild throughout many regions of Australia. The seeds of the plant contain the schedule 1 chemical agent ricin, a type II ribosomal inhibiting protein. Currently there are limited analytical techniques that can be applied in analysis of the seeds to establish attribution. In this study, laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) was used for the analysis of seeds collected from 68 plants across 38 locations around Australia. Of the 92 elemental isotopes measured, fifteen (24Mg,27Al,44Ca,53Cr,55Mn,57Fe,60Ni,65Cu,66Zn,75As,85Rb,88Sr,98Mo,138Ba and202Hg) yielded data that were relevant to all collection sites. Data were further analysed using multivariate statistical analysis which facilitated the potential for the identification of unique provenance isotopes. Furthermore, this analysis indicated that59Co was present at significant levels in Victorian and Sydney specimens only.
authors.affiliation: Defence Science and Technology Group, 506 Lorimer Street, Fishermans Bend, Victoria 3207, Australia.
authors.forename: Christina K
authors.initials: CK
authors.lastname: Bagas
authors.affiliation: TSW Analytical Pty Ltd., 2/27 Clark Court, Bibra Lake, Western Australia 6163, Australia. Electronic address: rachel@tswanalytical.com.au.
authors.forename: Rachel L
authors.initials: RL
authors.lastname: Scadding
authors.affiliation: TSW Analytical Pty Ltd., 2/27 Clark Court, Bibra Lake, Western Australia 6163, Australia.
authors.forename: Cameron J
authors.initials: CJ
authors.lastname: Scadding
authors.affiliation: TSW Analytical Pty Ltd., 2/27 Clark Court, Bibra Lake, Western Australia 6163, Australia.
authors.forename: R John
authors.initials: RJ
authors.lastname: Watling
authors.affiliation: Defence Science and Technology Group, 506 Lorimer Street, Fishermans Bend, Victoria 3207, Australia.
authors.forename: Warren
authors.initials: W
authors.lastname: Roberts
authors.affiliation: Defence Science and Technology Group, 506 Lorimer Street, Fishermans Bend, Victoria 3207, Australia. Electronic address: simon.ovenden@dsto.defence.gov.au.
authors.forename: Simon P B
authors.initials: SPB
authors.lastname: Ovenden
mesh_terms: D001315:Australia; D044707:Forensic Sciences / Q000379:methods; D007554:Isotopes / Q000032:analysis*; D053844:Lasers, Solid-State; D013058:Mass Spectrometry / Q000379:methods*; D012278:Ricinus; D012639:Seeds / Q000737:chemistry*
chemical_list: D007554:Isotopes
keywords: Laser ablation-ICP-MS; Multivariate statistical analysis; Provenance; Ricin; Ricinus communis; Trace isotope