title: Design of a hydroxyapatite-binding antimicrobial peptide with improved retention and antibacterial efficacy for oral pathogen control.
abstract: Controlling and reducing the formation of pathogenic biofilm on tooth surface is the key to the prevention and treatment of the biofilm-associated oral diseases. Antimicrobial peptides (AMPs), considered as possible future alternatives for conventional antibiotics, have been extensively studied for the control of bacterial infection. Due to the rapid dilution and degradation by human saliva, AMP preparations designed for oral use with longer retention and higher efficacy are in urgent need. To this end, a hydroxyapatite (HAp)-binding antimicrobial peptide (HBAMP), which is based on the fusion of a specific HAp-binding heptapeptide (HBP7) domain and a broad-spectrum antimicrobial peptide (KSLW) domain, has been developed in our laboratory. HBAMP was supposed to form a contact-active antibacterial interface on tooth surface to inhibit the formation of biofilms. In this study, we investigated its binding behaviour, antibacterial activity against bacteria in both planktonic and sessile states, enzymatic stability in human saliva, and cytocompatibility to human gingival fibroblasts (HGFs). Our findings suggest that HBAMP could adsorb on tooth surface to provide effective antibacterial activity with improved retention. This study provides a proof-of-concept on using conjugated molecules to promote antibacterial efficacy by synergistically actions of HBAMP free in solution and bound on tooth surface.
authors.affiliation: Department of Stomatology, Zhongshan Hospital, Xiamen University, Xiamen 361004, P. R. China.
authors.forename: Zhi-Bin
authors.initials: ZB
authors.lastname: Huang
authors.affiliation: Centre of Stomatology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, P.R. China.
authors.forename: Xin
authors.initials: X
authors.lastname: Shi
authors.affiliation: Centre of Stomatology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, P.R. China.
authors.forename: Jing
authors.initials: J
authors.lastname: Mao
authors.affiliation: Centre of Stomatology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, P.R. China.
authors.forename: Shi-Qiang
authors.initials: SQ
authors.lastname: Gong
mesh_terms: D016924:Actinomyces viscosus / Q000187:drug effects / Q000254:growth & development; D000595:Amino Acid Sequence; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D023181:Antimicrobial Cationic Peptides / Q000737:chemistry / Q000494:pharmacology*; D018441:Biofilms / Q000187:drug effects* / Q000254:growth & development; D002478:Cells, Cultured; D003692:Delayed-Action Preparations / Q000737:chemistry / Q000494:pharmacology*; D017886:Durapatite / Q000737:chemistry*; D005347:Fibroblasts / Q000187:drug effects / Q000382:microbiology / Q000473:pathology; D005881:Gingiva / Q000166:cytology / Q000382:microbiology; D006801:Humans; D007779:Lactobacillus acidophilus / Q000187:drug effects / Q000254:growth & development; D008826:Microbial Sensitivity Tests; D010933:Plankton / Q000187:drug effects* / Q000254:growth & development; D011485:Protein Binding; D012463:Saliva / Q000737:chemistry / Q000382:microbiology; D013295:Streptococcus mutans / Q000187:drug effects / Q000254:growth & development; D013298:Streptococcus sanguis / Q000187:drug effects / Q000254:growth & development
chemical_list: D000900:Anti-Bacterial Agents; D023181:Antimicrobial Cationic Peptides; D003692:Delayed-Action Preparations; D017886:Durapatite