title: System maps for retention of small neutral compounds on a biphenylsiloxane-bonded silica stationary phase in reversed-phase liquid chromatography.
abstract: The system constants of the solvation parameter model are used to prepare system maps for the retention of small neutral compounds on a biphenylsiloxane-bonded superficially porous silica stationary phase (Kinetex Biphenyl) for aqueous-organic solvent mobile phases containing 10-70% (v/v) methanol or acetonitrile. The retention properties of the biphenylsiloxane-bonded phase are shown to be complementary to an octadecylsiloxane-bonded silica (Kinetex C-18) and a pentafluorophenylpropylsiloxane-bonded silica stationary phases (Discovery HS F5). The retention properties of the Kinetex Biphenyl column are similar to an ether-linked phenylpropylsiloxane-bonded silica phase (Synergi Polar RP) with only small differences in relative retention.
authors.affiliation: CanAm Bioresearch Inc., Winnipeg, MB, R3T 0P4, Canada.
authors.forename: Sanka N
authors.initials: SN
authors.lastname: Atapattu
authors.affiliation: Department of Chemistry, Wayne State University, Detroit, MI, 48202, USA. Electronic address: cfp@chem.wayne.edu.
authors.forename: Colin F
authors.initials: CF
authors.lastname: Poole
authors.affiliation: Phenomenex Inc., Torrance, CA, 90501, USA.
authors.forename: Mike B
authors.initials: MB
authors.lastname: Praseuth
mesh_terms: D000097:Acetonitriles / Q000737:chemistry; D002623:Chemistry Techniques, Analytical / Q000379:methods*; D056148:Chromatography, Reverse-Phase; D000432:Methanol / Q000737:chemistry; D010648:Phenylacetates / Q000737:chemistry; D016062:Porosity; D012822:Silicon Dioxide / Q000737:chemistry*; D012997:Solvents / Q000737:chemistry; D014867:Water / Q000737:chemistry
chemical_list: D000097:Acetonitriles; D010648:Phenylacetates; D012997:Solvents; D014867:Water; C012776:biphenylylacetic acid; D012822:Silicon Dioxide; D000432:Methanol
keywords: Column characterization; Retention; Retention mapping; Reversed-phase liquid chromatography; Selectivity; Solvation parameter model; System maps