title: Acyl homoserine lactone changes the abundance of proteins and the levels of organic acids associated with stationary phase in Salmonella Enteritidis.
abstract: Quorum sensing (QS) is cell-cell communication mechanism mediated by signaling molecules known as autoinducers (AIs) that lead to differential gene expression. Salmonella is unable to synthesize the AI-1 acyl homoserine lactone (AHL), but is able to recognize AHLs produced by other microorganisms through SdiA protein. Our study aimed to evaluate the influence of AI-1 on the abundance of proteins and the levels of organic acids of Salmonella Enteritidis. The presence of N-dodecyl-homoserine lactone (C12-HSL) did not interfere on the growth or the total amount of extracted proteins of Salmonella. However, the abundance of the proteins PheT, HtpG, PtsI, Adi, TalB, PmgI (or GpmI), Eno, and PykF enhanced while the abundance of the proteins RplB, RplE, RpsB, Tsf, OmpA, OmpC, OmpD, and GapA decreased when Salmonella Enteritidis was anaerobically cultivated in the presence of C12-HSL. Additionally, the bacterium produced less succinic, lactic, and acetic acids in the presence of C12-HSL. However, the concentration of extracellular formic acid reached 20.46 mM after 24 h and was not detected when the growth was in the absence of AI-1. Considering the cultivation period for protein extraction, their abundance, process and function, as well as the levels of organic acids, we observed in cells cultivated in presence of C12-HSL a correlation with what is described in the literature as entry into the stationary phase of growth, mainly related to nitrogen and amino acid starvation and acid stress. Further studies are needed in order to determine the specific role of the differentially abundant proteins and extracellular organic acids secreted by Salmonella in the presence of quorum sensing signaling molecules.
authors.affiliation: Department of Microbiology, Universidade Federal de Viçosa, Viçosa, MG, Brazil.
authors.forename: Felipe Alves
authors.initials: FA
authors.lastname: de Almeida
authors.affiliation: Center of Nature Science, Universidade Federal de São Carlos, Buri, SP, Brazil.
authors.forename: Natan de Jesus
authors.initials: NJ
authors.lastname: Pimentel-Filho
authors.affiliation: Department of Biochemistry and Molecular Biology, Universidade Federal de Viçosa, Viçosa, MG, Brazil.
authors.forename: Lanna Clícia
authors.initials: LC
authors.lastname: Carrijo
authors.affiliation: Institute of Agrarian Science, Universidade Federal dos Vales do Jequitinhonha e Mucuri, Unaí, MG, Brazil.
authors.forename: Cláudia Braga Pereira
authors.initials: CBP
authors.lastname: Bento
authors.affiliation: Department of Biochemistry and Molecular Biology, Universidade Federal de Viçosa, Viçosa, MG, Brazil.
authors.forename: Maria Cristina
authors.initials: MC
authors.lastname: Baracat-Pereira
authors.affiliation: Food Research Center, Department of Food and Experimental Nutrition, Faculty of Pharmaceutical Sciences, Universidade de São Paulo, São Paulo, SP, Brazil.
authors.forename: Uelinton Manoel
authors.initials: UM
authors.lastname: Pinto
authors.affiliation: Department of General Biology, Universidade Federal de Viçosa, Viçosa, MG, Brazil.
authors.forename: Leandro Licursi
authors.initials: LL
authors.lastname: de Oliveira
authors.affiliation: Department of Microbiology, Universidade Federal de Viçosa, Viçosa, MG, Brazil. Electronic address: mvanetti@ufv.br.
authors.forename: Maria Cristina Dantas
authors.initials: MCD
authors.lastname: Vanetti
mesh_terms: D015107:4-Butyrolactone / Q000031:analogs & derivatives* / Q000494:pharmacology; D000143:Acids / Q000378:metabolism*; D001426:Bacterial Proteins / Q000378:metabolism*; D000431:Ethanol / Q000378:metabolism; D025941:Protein Interaction Mapping; D060066:Protein Interaction Maps; D040901:Proteomics / Q000379:methods; D053038:Quorum Sensing; D012477:Salmonella enteritidis / Q000187:drug effects* / Q000254:growth & development / Q000502:physiology*
chemical_list: D000143:Acids; D001426:Bacterial Proteins; C088386:homoserine lactone; D000431:Ethanol; D015107:4-Butyrolactone
keywords: Autoinducer; Differentially abundant proteins; Formic acid; Starvation; Stationary phase