title: Multidimensional liquid-phase separations combining both chromatography and electrophoresis - A review.
abstract: Described as intrinsically powerful building blocks for two-dimensional separations by Giddings [Anal. Chem., 56 (1984) 1258A-1270A], the coupling of chromatography and electrophoresis has been proven to enhance the resolution of a wide array of molecules in complex biological, environmental and food samples. This review provides a comprehensive overview of multidimensional chromato-electrophoretic (LC - E) and electrophero-chromatographic (E - LC) separation systems from inception to the most recent published examples. LC separation modes include reversed phase, ion exchange, and size exclusion. Electromigration separation modes include capillary, microchip or free flow electrophoresis; micellar electrokinetic chromatography; electrochromatography; and isoelectric focusing. The advantages and disadvantages of various non-gel based off-line and on-line hyphenation technologies of LC - E and E - LC are discussed, with conditions and system characteristics also provided.
authors.affiliation: Australian Centre for Research on Separation Science, School of Physical Sciences, University of Tasmania, Private Bag 75, Hobart, Tasmania 7001, Australia.
authors.forename: Leila
authors.initials: L
authors.lastname: Ranjbar
authors.affiliation: Australian Centre for Research on Separation Science, School of Physical Sciences, University of Tasmania, Private Bag 75, Hobart, Tasmania 7001, Australia; Department of Chemistry, Drexel University, Philadelphia, PA 19104, USA.
authors.forename: Joe P
authors.initials: JP
authors.lastname: Foley
authors.affiliation: Australian Centre for Research on Separation Science, School of Physical Sciences, University of Tasmania, Private Bag 75, Hobart, Tasmania 7001, Australia. Electronic address: Michael.Breadmore@utas.edu.au.
authors.forename: Michael C
authors.initials: MC
authors.lastname: Breadmore
keywords: Chromatography; Comprehensive; Electrophoresis; Heart-cutting; Multidimensional separation; Two-dimensional electrophoresis-liquid chromatography; Two-dimensional liquid chromatography-electrophoresis