title: Distinct Contributory Factors Determine Basophil-Allergen Sensitivity in Grass Pollen Rhinitis and in Anaphylactic Wasp Venom Allergy.
abstract: BACKGROUND
  We sought to determine whether basophil-allergen sensitivity could be transferred to donor basophils by passive IgE sensitisation in allergic rhinitis and anaphylactic Hymenoptera venom hypersensitivity.
  
  
  METHODS
  We studied 15 wasp venom-, 19 grass pollen- and 2 house dust mite-allergic patients, 2 healthy donors, and 8 wasp venom-allergic donors. In all subjects, we first evaluated the initial basophil response to wasp venom, grass pollen, or house dust mite allergen. Donor basophils were then stripped, sensitised with the different patients' serum IgE, and challenged with the corresponding allergen. The CD63 response of donor basophils was then compared with initial basophil responses.
  
  
  RESULTS
  In wasp venom-allergic subjects, the IgE transfer did not reflect the initial basophil-allergen sensitivity, because the venom IgE of subjects with high or low basophil sensitivity induced comparable responsiveness in healthy donor basophils. Furthermore, vice versa, when we sensitised the donor basophils of wasp venom-allergic individuals with different wasp venom or house dust mite IgE, we demonstrated that their response was predictable by their initial basophil allergen sensitivity. In the rhinitis allergy model, the IgE transfer correlated with the patients' initial basophil responsiveness because the grass pollen IgE of the subjects with high basophil allergen sensitivity induced significantly higher responsiveness of donor basophils than the IgE of subjects with initially low basophil allergen sensitivity.
  
  
  CONCLUSIONS
  Our results suggest that basophil allergen sensitivity evaluated by flow-cytometric CD63 analysis depends on two distinct contribution factors. In anaphylactic Hymenoptera allergy, the major factor was intrinsic cellular sensitivity, whereas in pollen allergy, the major factor was allergen-specific IgE on the cell surface.
authors.affiliation: University Clinic of Respiratory and Allergic Diseases, Golnik, Slovenia.
authors.forename: Peter
authors.initials: P
authors.lastname: Korošec
authors.forename: Mira
authors.initials: M
authors.lastname: Šilar
authors.forename: Peter
authors.initials: P
authors.lastname: Kopač
authors.forename: Renato
authors.initials: R
authors.lastname: Eržen
authors.forename: Mihaela
authors.initials: M
authors.lastname: Zidarn
authors.forename: Mitja
authors.initials: M
authors.lastname: Košnik
mesh_terms: D000293:Adolescent; D000328:Adult; D000485:Allergens / Q000276:immunology*; D000707:Anaphylaxis / Q000175:diagnosis / Q000276:immunology*; D000818:Animals; D000918:Antibody Specificity / Q000276:immunology; D001491:Basophils / Q000276:immunology*; D016022:Case-Control Studies; D003429:Cross Reactions / Q000276:immunology; D005260:Female; D006801:Humans; D007116:Immunization, Passive; D007073:Immunoglobulin E / Q000097:blood / Q000276:immunology; D008297:Male; D008875:Middle Aged; D011058:Pollen / Q000276:immunology*; D039981:Pyroglyphidae / Q000276:immunology; D011961:Receptors, Fc / Q000378:metabolism; D006255:Rhinitis, Allergic, Seasonal / Q000175:diagnosis / Q000276:immunology*; D014862:Wasp Venoms / Q000276:immunology*; D055815:Young Adult
chemical_list: D000485:Allergens; D011961:Receptors, Fc; D014862:Wasp Venoms; D007073:Immunoglobulin E