title: Determination of fluoxetine in Dermestes maculatus (Coleoptera: Dermestidae) by a spectrophotometric method.
abstract: The aims of this study were to detect and quantify fluoxetine, an antidepressant, from entomological samples. Larvae, pupae and adults of Dermestes maculatus (Coleoptera, Dermestidae) were reared on pig muscle previously treated with fluoxetine. The concentration selected, 2000mg/kg, emulates a fluoxetine overdose lethal to humans and laboratory animals. Thirty larvae on the fourth and fifth stages, 50 adults and several exuviae were analyzed for fluoxetine content. Detection of fluoxetine was performed by UV spectrophotometry at 270 and 277nm. All developmental stages of D. maculatus and exuviae were positive for fluoxetine. We also quantified the drug and no significant differences were found either between the days or the stages in the general model, but at 277nm a tendency of the concentration to decrease with time was observed. Concentrations of fluoxetine at 277nm were almost equal or greater than those at 270nm. This is the first study to detect and quantify fluoxetine from entomological samples and, in particular, from D. maculatus beetles.
authors.affiliation: Laboratorio de Entomología Aplicada y Forense, Departamento de Ciencia y Técnica, Universidad Nacional de Quilmes, Roque Sáenz Peña 352, Bernal 1876, Provincia de Buenos Aires, Argentina; Cátedra de Zoología de Invertebrados II, Departamento de Biología, Bioquímica y Farmacia, Universidad Nacional del Sur, San Juan 670, Bahía Blanca 8000, Provincia de Buenos Aires, Argentina. Electronic address: noeinesz@yahoo.com.ar.
authors.forename: Noelia I
authors.initials: NI
authors.lastname: Zanetti
authors.affiliation: Cátedra de Zoología de Invertebrados II, Departamento de Biología, Bioquímica y Farmacia, Universidad Nacional del Sur, San Juan 670, Bahía Blanca 8000, Provincia de Buenos Aires, Argentina; INBIOSUR (Instituto de Investigaciones Biológicas y Biomédicas del Sur), Universidad Nacional del Sur, San Juan 670, Bahía Blanca 8000, Provincia de Buenos Aires, Argentina.
authors.forename: Adriana A
authors.initials: AA
authors.lastname: Ferrero
authors.affiliation: Laboratorio de Entomología Aplicada y Forense, Departamento de Ciencia y Técnica, Universidad Nacional de Quilmes, Roque Sáenz Peña 352, Bernal 1876, Provincia de Buenos Aires, Argentina.
authors.forename: Néstor D
authors.initials: ND
authors.lastname: Centeno
mesh_terms: D000818:Animals; D018687:Antidepressive Agents, Second-Generation / Q000032:analysis*; D001517:Coleoptera; D005247:Feeding Behavior; D005473:Fluoxetine / Q000032:analysis*; D049429:Forensic Pathology; D053593:Forensic Toxicology; D007814:Larva; D011180:Postmortem Changes; D011679:Pupa; D013056:Spectrophotometry, Ultraviolet; D013552:Swine
chemical_list: D018687:Antidepressive Agents, Second-Generation; D005473:Fluoxetine
keywords: Beetles; Dermestes maculatus; Entomotoxicology; Fluoxetine; Spectrophotometry