title: Features of endothelial dysfunction and morphofunctional changes of the uteroplacental complex in experimentally induced pre-eclampsia.
abstract: BACKGROUND
  Pre-eclampsia is considered to be a severe complication of pregnancy. Theoretical investigation of its etiology and pathogenesis, development of strategies for its prevention and treatment are conditioned by the development of appropriate experimental models of this pathology.
  
  
  METHODS
  The study involved Wistar rat lines weighing 220-240g. Experimental pre-eclampsia was modeled by replacing drinking water consumed by pregnant female rats with 1.8% NaCl solution throughout gestation. Arterial pressure, protein concentration in urine and tissue hydration extent were measured on the 1st and 21st days of gestation. Uteroplacental blood flow, vasodilating and antithrombotic endothelial functions were also assessed. For pathomorphological and immunohistochemical investigation murine monoclonal antibodies against vascular endothelial growth factor (VEGF), polyclonal rabbit antibodies against inducible and endothelial NO-synthases were used.
  
  
  RESULTS
  Replacing drinking water with 1.8% NaCl solution in female rats throughout gestation elevates arterial pressure, causes proteinuria and edema, impairs vasodilating and antithrombotic endothelial properties, and suppresses uteroplacental blood circulation. A morphological examination of the animals revealed the signs of focal duodenitis, spasms of myometrium arteries with no invasion of syncytiotrophoblast into its walls which also involved a raised VEGF and reduced eNOS expression in the endothelium of myometrial vessels, as well as cytoplasmic expression of iNOS in the cells of inflammatory infiltrate.
  
  
  CONCLUSIONS
  These findings make it possible to conclude that replacing drinking water with 1.8% NaCl solution causes a number of changes typical of pre-eclampsia and, therefore, can be regarded as an experimental model of this pathologic condition.
authors.affiliation: Department of Pharmacology and Biopharmacy of the Department for Refresher Training, Volgograd State Medical University, 400001 Volgograd, Russia.
authors.forename: I N
authors.initials: IN
authors.lastname: Tyurenkov
authors.affiliation: Department of Pharmacology and Biopharmacy of the Department for Refresher Training, Volgograd State Medical University, 400001 Volgograd, Russia. Electronic address: vnperfilova@gmail.com.
authors.forename: V N
authors.initials: VN
authors.lastname: Perfilova
authors.affiliation: Department of Pathological Anatomy, Volgograd State Medical University, Volgograd, Russia.
authors.forename: A V
authors.initials: AV
authors.lastname: Smirnov
authors.affiliation: Department of Pharmacology and Biopharmacy of the Department for Refresher Training, Volgograd State Medical University, 400001 Volgograd, Russia.
authors.forename: L B
authors.initials: LB
authors.lastname: Reznikova
authors.affiliation: Department of Pharmacology and Biopharmacy of the Department for Refresher Training, Volgograd State Medical University, 400001 Volgograd, Russia.
authors.forename: A V
authors.initials: AV
authors.lastname: Poroyskaya
authors.affiliation: Department of Theoretical Biochemistry with a Course of Clinical Biochemistry, Volgograd State Medical University, Volgograd, Russia.
authors.forename: V E
authors.initials: VE
authors.lastname: Verovsky
mesh_terms: D000818:Animals; D062186:Arterial Pressure; D001834:Body Water; D004195:Disease Models, Animal; D004727:Endothelium / Q000503:physiopathology*; D004730:Endothelium, Vascular / Q000503:physiopathology*; D005260:Female; D052247:Nitric Oxide Synthase Type II / Q000378:metabolism; D052250:Nitric Oxide Synthase Type III / Q000378:metabolism; D010920:Placenta / Q000098:blood supply / Q000473:pathology / Q000503:physiopathology*; D021041:Placental Circulation; D011225:Pre-Eclampsia / Q000139:chemically induced / Q000503:physiopathology*; D011247:Pregnancy; D011507:Proteinuria / Q000209:etiology; D017208:Rats, Wistar; D012965:Sodium Chloride; D014599:Uterus / Q000098:blood supply / Q000473:pathology / Q000503:physiopathology*; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism; D014664:Vasodilation
chemical_list: D042461:Vascular Endothelial Growth Factor A; C467498:vascular endothelial growth factor A, rat; D012965:Sodium Chloride; D052247:Nitric Oxide Synthase Type II; D052250:Nitric Oxide Synthase Type III
keywords: Experimental model pre-eclampsia endothelial dysfunction; Morphology; Pharmacology