title: Recombinant Bacillus subtilis spores expressing cholera toxin B subunit and Helicobacter pylori urease B confer protection against H. pylori in mice.
abstract: Helicobacter pylori infection is associated with chronic gastritis, peptic ulcers, gastric cancer and mucosa-associated lymphoid tissue lymphoma. The limitations of current therapies for H. pylori infection include poor compliance and antibiotic resistance. Therefore, an effective anti-H. pylori vaccine would be an alternative or complement to antibiotic treatment. Urease B (UreB) is considered an ideal vaccine antigen against H. pylori infection. In this study, cholera toxin B subunit (CTB), a mucosal adjuvant, was used to enhance the immunogenicity of a novel Bacillus subtilis spore vaccine expressing CTB-UreB, along with the B. subtilis spore coat protein CotC as a fusion protein. Oral administration of B. subtilis spores expressing CotC-UreB or CotC-CTB-UreB led to increased levels of UreB-specific IgG in serum and UreB-specific IgA in faeces, as well as elevated levels of IL-10 and IFN-γ in splenocytes. In addition, oral administration of CotC-UreB or CotC-CTB-UreB spores induced significant reductions (80.0 and 90.5 %, respectively) in gastric H. pylori bacterial load (1.11±0.36×105 and 0.53±0.21×105 c.f.u., respectively) compared to that of the CotC control group (5.56±1.64×105 c.f.u., P<0.01). Moreover, CotC-CTB-UreB spores were significantly more effective at reducing the bacterial load than CotC-UreB spores (P<0.05). These results indicate that CotC-CTB-UreB-expressing B. subtilis spores are a potential vaccine candidate for the control of H. pylori infection.
authors.affiliation: Guangzhou Women and Children's Medical Center, Guangzhou Medical University, No. 318 Renminzhong Road, Yuexiu, Guangzhou,Guangdong 510120, PR China.
authors.forename: Zhenwen
authors.initials: Z
authors.lastname: Zhou
authors.affiliation: Guangzhou Women and Children's Medical Center, Guangzhou Medical University, No. 318 Renminzhong Road, Yuexiu, Guangzhou,Guangdong 510120, PR China.
authors.forename: Hui
authors.initials: H
authors.lastname: Dong
authors.affiliation: Guangzhou Women and Children's Medical Center, Guangzhou Medical University, No. 318 Renminzhong Road, Yuexiu, Guangzhou,Guangdong 510120, PR China.
authors.forename: Yanmei
authors.initials: Y
authors.lastname: Huang
authors.affiliation: Guangzhou Women and Children's Medical Center, Guangzhou Medical University, No. 318 Renminzhong Road, Yuexiu, Guangzhou,Guangdong 510120, PR China.
authors.forename: Shuwen
authors.initials: S
authors.lastname: Yao
authors.affiliation: Guangzhou Women and Children's Medical Center, Guangzhou Medical University, No. 318 Renminzhong Road, Yuexiu, Guangzhou,Guangdong 510120, PR China.
authors.forename: Bingshao
authors.initials: B
authors.lastname: Liang
authors.affiliation: Guangzhou Women and Children's Medical Center, Guangzhou Medical University, No. 318 Renminzhong Road, Yuexiu, Guangzhou,Guangdong 510120, PR China.
authors.forename: Yongqiang
authors.initials: Y
authors.lastname: Xie
authors.affiliation: Guangzhou Women and Children's Medical Center, Guangzhou Medical University, No. 318 Renminzhong Road, Yuexiu, Guangzhou,Guangdong 510120, PR China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Long
authors.affiliation: Guangzhou Women and Children's Medical Center, Guangzhou Medical University, No. 318 Renminzhong Road, Yuexiu, Guangzhou,Guangdong 510120, PR China.
authors.forename: Jialiang
authors.initials: J
authors.lastname: Mai
authors.affiliation: Guangzhou Women and Children's Medical Center, Guangzhou Medical University, No. 318 Renminzhong Road, Yuexiu, Guangzhou,Guangdong 510120, PR China.
authors.forename: Sitang
authors.initials: S
authors.lastname: Gong
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000276:immunology; D001412:Bacillus subtilis / Q000276:immunology*; D058491:Bacterial Load; D001426:Bacterial Proteins / Q000235:genetics / Q000276:immunology*; D001428:Bacterial Vaccines / Q000276:immunology; D002772:Cholera Toxin / Q000235:genetics / Q000276:immunology*; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000235:genetics; D005260:Female; D016481:Helicobacter Infections / Q000276:immunology / Q000517:prevention & control*; D016480:Helicobacter pylori / Q000201:enzymology* / Q000276:immunology; D007070:Immunoglobulin A / Q000276:immunology; D007074:Immunoglobulin G / Q000276:immunology; D007371:Interferon-gamma / Q000276:immunology; D016753:Interleukin-10 / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology; D013154:Spleen / Q000166:cytology / Q000276:immunology; D013171:Spores, Bacterial / Q000276:immunology; D014510:Urease / Q000235:genetics / Q000276:immunology*
chemical_list: D000907:Antibodies, Bacterial; D001426:Bacterial Proteins; D001428:Bacterial Vaccines; D004269:DNA, Bacterial; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D011994:Recombinant Proteins; D016753:Interleukin-10; D007371:Interferon-gamma; D002772:Cholera Toxin; D014510:Urease