title: The Core Subunit of A Chromatin-Remodeling Complex, ZmCHB101, Plays Essential Roles in Maize Growth and Development.
abstract: ATP-dependent chromatin remodeling complexes play essential roles in the regulation of diverse biological processes by formulating a DNA template that is accessible to the general transcription apparatus. Although the function of chromatin remodelers in plant development has been studied in A. thaliana, how it affects growth and development of major crops (e.g., maize) remains uninvestigated. Combining genetic, genomic and bioinformatic analyses, we show here that the maize core subunit of chromatin remodeling complex, ZmCHB101, plays essential roles in growth and development of maize at both vegetative and reproductive stages. Independent ZmCHB101 RNA interference plant lines displayed abaxially curling leaf phenotype due to increase of bulliform cell numbers, and showed impaired development of tassel and cob. RNA-seq-based transcriptome profiling revealed that ZmCHB101 dictated transcriptional reprogramming of a significant set of genes involved in plant development, photosynthesis, metabolic regulation, stress response and gene expressional regulation. Intriguingly, we found that ZmCHB101 was required for maintaining normal nucleosome density and 45 S rDNA compaction. Our findings suggest that the SWI3 protein, ZmCHB101, plays pivotal roles in maize normal growth and development via regulation of chromatin structure.
authors.affiliation: Key Laboratory of Molecular Epigenetics of the Ministry of Education (MOE), Northeast Normal University, Changchun 130024, P. R. China.|School of Bioengineering, Jilin College of Agricultural Science &Technology, Jilin 132301, P. R. China.
authors.forename: Xiaoming
authors.initials: X
authors.lastname: Yu
authors.affiliation: Key Laboratory of Molecular Epigenetics of the Ministry of Education (MOE), Northeast Normal University, Changchun 130024, P. R. China.
authors.forename: Lili
authors.initials: L
authors.lastname: Jiang
authors.affiliation: Key Laboratory of Molecular Epigenetics of the Ministry of Education (MOE), Northeast Normal University, Changchun 130024, P. R. China.
authors.forename: Rui
authors.initials: R
authors.lastname: Wu
authors.affiliation: Key Laboratory of Molecular Epigenetics of the Ministry of Education (MOE), Northeast Normal University, Changchun 130024, P. R. China.
authors.forename: Xinchao
authors.initials: X
authors.lastname: Meng
authors.affiliation: Key Laboratory of Molecular Epigenetics of the Ministry of Education (MOE), Northeast Normal University, Changchun 130024, P. R. China.
authors.forename: Ai
authors.initials: A
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Molecular Epigenetics of the Ministry of Education (MOE), Northeast Normal University, Changchun 130024, P. R. China.
authors.forename: Ning
authors.initials: N
authors.lastname: Li
authors.affiliation: Key Laboratory of Molecular Epigenetics of the Ministry of Education (MOE), Northeast Normal University, Changchun 130024, P. R. China.
authors.forename: Qiong
authors.initials: Q
authors.lastname: Xia
authors.affiliation: Department of Agronomy, Jilin Agricultural University, Changchun 130118, P. R. China.
authors.forename: Xin
authors.initials: X
authors.lastname: Qi
authors.affiliation: Key Laboratory of Molecular Epigenetics of the Ministry of Education (MOE), Northeast Normal University, Changchun 130024, P. R. China.
authors.forename: Jinsong
authors.initials: J
authors.lastname: Pang
authors.affiliation: Key Laboratory of Molecular Epigenetics of the Ministry of Education (MOE), Northeast Normal University, Changchun 130024, P. R. China.
authors.forename: Zheng-Yi
authors.initials: ZY
authors.lastname: Xu
authors.affiliation: Key Laboratory of Molecular Epigenetics of the Ministry of Education (MOE), Northeast Normal University, Changchun 130024, P. R. China.
authors.forename: Bao
authors.initials: B
authors.lastname: Liu
mesh_terms: D042002:Chromatin Assembly and Disassembly; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D053263:Gene Regulatory Networks; D017343:Genes, Plant; D009707:Nucleosomes / Q000378:metabolism; D063245:Plant Development; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D018517:Plant Roots / Q000235:genetics; D018520:Plant Shoots / Q000235:genetics; D030821:Plants, Genetically Modified; D021122:Protein Subunits / Q000378:metabolism*; D034622:RNA Interference; D060888:Real-Time Polymerase Chain Reaction; D015203:Reproducibility of Results; D014158:Transcription, Genetic; D003313:Zea mays / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism*
chemical_list: D009707:Nucleosomes; D010940:Plant Proteins; D021122:Protein Subunits