title: Modulation of adiponectin system expression in the porcine uterus during early pregnancy by prostaglandin E2and F2α.
abstract: Studies have demonstrated that adiponectin could be a link between reproductive functions and energy metabolism in animals. The aim of the present study was to investigate the effects of prostaglandin (PG) E2and PGF2α(10, 50, 100, 250 and 500ngmL-1) on the expression and secretion of adiponectin and its receptor genes and proteins by cultured in vitro porcine endometrial and myometrial tissues on Days 10-28 of pregnancy and Days 10-11 of the oestrous cycle. The gene expression was analysed using the real-time PCR method. Adiponectin protein secretion was determined by ELISA, whereas the receptors proteins content was defined using Western Blot analysis. Both PGE2and PGF2αmodulated the expression of adiponectin system genes and proteins in the uterus during early pregnancy. PGE2and PGF2αhad similar effects on the adiponectin system, which differed between the stages of gestation and between pregnancy and the oestrous cycle. On Days 10-11 of gestation, PGE2and PGF2αgenerally increased adiponectin secretion by endometrial and myometrial tissues. Both PGs decreased levels of endometrial adiponectin receptor type 1 (AdipoR1), whereas only PGF2αdecreased myometrial levels of AdipoR1. Both PGs increased myometrial adiponectin receptor type 2 (AdipoR2) levels. On Days 12-13 of gestation, PGE2decreased AdipoR1 concentrations in both tissues and AdipoR2 levels in the endometrium. PGF2αdecreased myometrial concentrations of both receptors. On Days 15-16 of gestation, both PGE2and PGF2αincreased concentrations of AdipoR1 and AdipoR2 in the endometrium and myometrium. PGE2stimulated the secretion of adiponectin in the endometrium, but not in the myometrium. On Days 27-28 of pregnancy, both PGE2and PGF2αinhibited the expression of AdipoR1 and AdipoR2 in endometrial and myometrial tissues and decreased the secretion of endometrial adiponectin. Both PGE2and PGF2αhad tissue-specific and dose-dependent effects on the adiponectin system.
authors.affiliation: Department of Animal Physiology, Faculty of Biology and Biotechnology, University of Warmia and Mazury in Olsztyn, Oczapowskiego 1A, 10-719 Olsztyn-Kortowo, Poland.
authors.forename: Kamil
authors.initials: K
authors.lastname: Dobrzyn
authors.affiliation: Department of Animal Physiology, Faculty of Biology and Biotechnology, University of Warmia and Mazury in Olsztyn, Oczapowskiego 1A, 10-719 Olsztyn-Kortowo, Poland.
authors.forename: Nina
authors.initials: N
authors.lastname: Smolinska
authors.affiliation: Department of Animal Physiology, Faculty of Biology and Biotechnology, University of Warmia and Mazury in Olsztyn, Oczapowskiego 1A, 10-719 Olsztyn-Kortowo, Poland.
authors.forename: Karol
authors.initials: K
authors.lastname: Szeszko
authors.affiliation: Department of Animal Physiology, Faculty of Biology and Biotechnology, University of Warmia and Mazury in Olsztyn, Oczapowskiego 1A, 10-719 Olsztyn-Kortowo, Poland.
authors.forename: Marta
authors.initials: M
authors.lastname: Kiezun
authors.affiliation: Department of Animal Physiology, Faculty of Biology and Biotechnology, University of Warmia and Mazury in Olsztyn, Oczapowskiego 1A, 10-719 Olsztyn-Kortowo, Poland.
authors.forename: Anna
authors.initials: A
authors.lastname: Maleszka
authors.affiliation: Department of Animal Physiology, Faculty of Biology and Biotechnology, University of Warmia and Mazury in Olsztyn, Oczapowskiego 1A, 10-719 Olsztyn-Kortowo, Poland.
authors.forename: Tadeusz
authors.initials: T
authors.lastname: Kaminski
mesh_terms: D052242:Adiponectin / Q000378:metabolism*; D000818:Animals; D015237:Dinoprost / Q000494:pharmacology*; D015232:Dinoprostone / Q000494:pharmacology*; D005260:Female; D054419:Receptors, Adiponectin / Q000378:metabolism*; D013552:Swine; D014599:Uterus / Q000187:drug effects* / Q000378:metabolism
chemical_list: D052242:Adiponectin; D054419:Receptors, Adiponectin; D015237:Dinoprost; D015232:Dinoprostone