title: Characterization and fine-mapping of a novel premature leaf senescence mutant yellow leaf and dwarf 1 in rice.
abstract: Leaves are the main organs in which photosynthates are produced. Leaf senescence facilitates the translocation of photosynthates and nutrients from source to sink, which is important for plant development and especially for crop yield. However, the molecular mechanism of leaf senescence is unknown. Here, we identified a mutant, yellow leaf and dwarf 1 (yld1), which exhibited decreased plant height and premature leaf senescence. Nitroblue tetrazolium and diamiobenzidine staining analyses revealed that the concentrations of reactive oxygen species were higher in yld1 leaves than in wild type leaves. The photosynthetic pigment contents were significantly decreased in yld1. The yld1 chloroplasts had collapsed and were filled with abnormal starch granules. Combining bulk segregant and MutMap gene mapping approaches, the mutation responsible for the yld1 phenotype was mapped to a 7.3 Mb centromeric region, and three non-synonymous single nucleotide polymorphisms located in three novel genes were identified in this region. The expression patterns of the three candidate genes indicated that LOC_Os06g29380 had the most potential for functional verification. Plant hormone measurements showed that salicylic acid was highly accumulated in yld1 leaves when compared with wild type leaves, and yld1 was more sensitive to salicylic acid than wild type. This work lays the foundation for understanding the molecular regulatory mechanism of leaf senescence, and may reveal new connections among the molecular pathways related to leaf senescence, starch metabolism and salicylic acid signaling.
authors.affiliation: Rice Research Institute of Sichuan Agricultural University, Chengdu Wenjiang, Sichuan, 611130, China; Crop Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu, Sichuan, 610066, China.
authors.forename: Luchang
authors.initials: L
authors.lastname: Deng
authors.affiliation: Rice Research Institute of Sichuan Agricultural University, Chengdu Wenjiang, Sichuan, 611130, China; State Key Laboratory of Hybrid Rice, Sichuan Agricultural University, Chengdu Wenjiang, Sichuan, 611130, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Qin
authors.affiliation: Rice Research Institute of Sichuan Agricultural University, Chengdu Wenjiang, Sichuan, 611130, China; Agricultural Commission of Liupanshui City, Liupanshui, Guizhou, 553000, China.
authors.forename: Zhi
authors.initials: Z
authors.lastname: Liu
authors.affiliation: Rice Research Institute of Sichuan Agricultural University, Chengdu Wenjiang, Sichuan, 611130, China.
authors.forename: Geling
authors.initials: G
authors.lastname: Wang
authors.affiliation: Rice Research Institute of Sichuan Agricultural University, Chengdu Wenjiang, Sichuan, 611130, China.
authors.forename: Weilan
authors.initials: W
authors.lastname: Chen
authors.affiliation: Hunan Provincial Key Laboratory of Phytohormones and Growth Development, Hunan Agricultural University, Hunan, 410128, China.
authors.forename: Jianhua
authors.initials: J
authors.lastname: Tong
authors.affiliation: Hunan Provincial Key Laboratory of Phytohormones and Growth Development, Hunan Agricultural University, Hunan, 410128, China.
authors.forename: Langtao
authors.initials: L
authors.lastname: Xiao
authors.affiliation: Rice Research Institute of Sichuan Agricultural University, Chengdu Wenjiang, Sichuan, 611130, China; State Key Laboratory of Hybrid Rice, Sichuan Agricultural University, Chengdu Wenjiang, Sichuan, 611130, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Tu
authors.affiliation: Rice Research Institute of Sichuan Agricultural University, Chengdu Wenjiang, Sichuan, 611130, China.
authors.forename: Yuantao
authors.initials: Y
authors.lastname: Sun
authors.affiliation: College of Life Sciences, Peking University, Beijing, 100871, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Yan
authors.affiliation: College of Life Sciences, Peking University, Beijing, 100871, China.
authors.forename: Hang
authors.initials: H
authors.lastname: He
authors.affiliation: Crop Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu, Sichuan, 610066, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Tan
authors.affiliation: Rice Research Institute of Sichuan Agricultural University, Chengdu Wenjiang, Sichuan, 611130, China.
authors.forename: Xuewei
authors.initials: X
authors.lastname: Chen
authors.affiliation: Rice Research Institute of Sichuan Agricultural University, Chengdu Wenjiang, Sichuan, 611130, China; State Key Laboratory of Hybrid Rice, Sichuan Agricultural University, Chengdu Wenjiang, Sichuan, 611130, China.
authors.forename: Yuping
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Rice Research Institute of Sichuan Agricultural University, Chengdu Wenjiang, Sichuan, 611130, China; State Key Laboratory of Hybrid Rice, Sichuan Agricultural University, Chengdu Wenjiang, Sichuan, 611130, China. Electronic address: lishigui@sicau.edu.cn.
authors.forename: Shigui
authors.initials: S
authors.lastname: Li
authors.affiliation: Rice Research Institute of Sichuan Agricultural University, Chengdu Wenjiang, Sichuan, 611130, China; State Key Laboratory of Hybrid Rice, Sichuan Agricultural University, Chengdu Wenjiang, Sichuan, 611130, China. Electronic address: btma02@sicau.edu.cn.
authors.forename: Bingtian
authors.initials: B
authors.lastname: Ma
mesh_terms: D002736:Chloroplasts / Q000187:drug effects / Q000378:metabolism; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D017343:Genes, Plant; D056726:Genetic Association Studies; D058503:Mesophyll Cells / Q000166:cytology / Q000187:drug effects / Q000648:ultrastructure; D009154:Mutation / Q000235:genetics*; D012275:Oryza / Q000033:anatomy & histology / Q000235:genetics* / Q000254:growth & development* / Q000648:ultrastructure; D010641:Phenotype; D010788:Photosynthesis / Q000187:drug effects; D020161:Physical Chromosome Mapping / Q000379:methods*; D010860:Pigments, Biological / Q000378:metabolism; D010937:Plant Growth Regulators / Q000378:metabolism; D018515:Plant Leaves / Q000033:anatomy & histology / Q000235:genetics / Q000254:growth & development* / Q000648:ultrastructure; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D019655:Quantitative Trait, Heritable; D020156:Salicylic Acid / Q000494:pharmacology
chemical_list: D010860:Pigments, Biological; D010937:Plant Growth Regulators; D010940:Plant Proteins; D020156:Salicylic Acid
keywords: Chloroplast; Leaf senescence; MutMap; ROS; Reactive oxygen species; SA; SNP; Salicylic acid; Single nucleotide polymorphism; WT; Wild type; YLD; Yellow leaf and dwarf