title: Immunohistochemical Analysis in the Rat Central Nervous System and Peripheral Lymph Node Tissue Sections.
abstract: Immunohistochemistry (IHC) provides highly specific, reliable and attractive protein visualization. Correct performance and interpretation of an IHC-based multicolor labeling is challenging, especially when utilized for assessing interrelations between target proteins in the tissue with a high fat content such as the central nervous system (CNS). Our protocol represents a refinement of the standard immunolabeling technique particularly adjusted for detection of both structural and soluble proteins in the rat CNS and peripheral lymph nodes (LN) affected by neuroinflammation. Nonetheless, with or without further modifications, our protocol could likely be used for detection of other related protein targets, even in other organs and species than here presented.
authors.affiliation: Department of Clinical Neuroscience, Neuroimmunology Unit, Center for Molecular Medicine, Karolinska Institutet; Department of Neuroimmunology, Center for Brain Research, Medical University of Vienna; milena.zeitelhofer-adzemovic@ki.se.
authors.forename: Milena Z
authors.initials: MZ
authors.lastname: Adzemovic
authors.affiliation: Department of Clinical Neuroscience, Neuroimmunology Unit, Center for Molecular Medicine, Karolinska Institutet; Department of Medical Biochemistry and Biophysics, Vascular Biology Unit, Karolinska Institutet.
authors.forename: Manuel
authors.initials: M
authors.lastname: Zeitelhofer
authors.affiliation: Department of Neuroimmunology, Center for Brain Research, Medical University of Vienna.
authors.forename: Marianne
authors.initials: M
authors.lastname: Leisser
authors.affiliation: Department of Neuroimmunology, Center for Brain Research, Medical University of Vienna.
authors.forename: Ulricke
authors.initials: U
authors.lastname: Köck
authors.affiliation: Department of Neuroimmunology, Center for Brain Research, Medical University of Vienna.
authors.forename: Angela
authors.initials: A
authors.lastname: Kury
authors.affiliation: Department of Clinical Neuroscience, Neuroimmunology Unit, Center for Molecular Medicine, Karolinska Institutet.
authors.forename: Tomas
authors.initials: T
authors.lastname: Olsson
mesh_terms: D000818:Animals; D002490:Central Nervous System; D016591:Histocytological Preparation Techniques; D007150:Immunohistochemistry; D008198:Lymph Nodes; D051381:Rats