title: Interplay of genes in plant-pathogen interactions:In plantaexpression and docking studies of a beta 1,3 glucanase gene fromPiper colubrinumand a glucanase inhibitor gene fromPhytophthora capsici.
abstract: Oomycete pathogen,Phytophthora capsiciis devastating for black pepper (Piper nigrumL.) and causes foot rot disease at all stages of plant growth.Phytophthorasecretes a glucanase inhibitor protein (GIP), which is capable of inhibiting defence proteins like endoglucanases. In this particular study Quantitative PCR analysis, molecular docking studies and analysis of sequences of Glucanase inhibitor protein and beta-1,3 glucanse genes were done mainly depending on the data derived fromPhytophthora capsiciwhole genome sequencing andPiper colubrinumRNA-sequencing (RNA-Seq). Amino acid sequence length ofGIPgene fromP. capsiciwas about 353 amino acids and that of glucanasepcEGasegene fromP. colubrinumwas about 312 amino acids.GIPgene fromP. capsicishowed high level of expression at early hours of the inoculation time period and pcEGase gene showed high level of expression at 16 hpi. High level of expression ofpcEGasegene at 16 hpi is an indication that theGIPgene is successfully inhibited by the glucanase protein from the plant. Moreover insilico studies gave some hint on the importance of certain sites on the surfaces of both interacting proteins that might be having a role in binding of the two proteins and subsequent reactions thereof. Insilico analysis also conclusively proved that inhibition of glucanase inhibitor protein is mainly caused by recognition of an arginine as well as an isoleucine residue during the interaction of the two proteins.
authors.affiliation: Division of Crop Improvement and Division of Crop Protection, Indian Institute of Spices Research, Calicut, 673012 India.
authors.forename: K
authors.initials: K
authors.lastname: Johnson George
authors.affiliation: Division of Crop Improvement and Division of Crop Protection, Indian Institute of Spices Research, Calicut, 673012 India.
authors.forename: O
authors.initials: O
authors.lastname: Rosana Babu
authors.affiliation: Division of Crop Improvement and Division of Crop Protection, Indian Institute of Spices Research, Calicut, 673012 India.
authors.forename: I P
authors.initials: IP
authors.lastname: Vijesh Kumar
authors.affiliation: Division of Crop Improvement and Division of Crop Protection, Indian Institute of Spices Research, Calicut, 673012 India.
authors.forename: J
authors.initials: J
authors.lastname: Santhosh Eapen
authors.affiliation: Division of Crop Improvement and Division of Crop Protection, Indian Institute of Spices Research, Calicut, 673012 India.
authors.forename: M
authors.initials: M
authors.lastname: Anandaraj
keywords: Black pepper; Molecular docking; Phytophthora capsici; Piper colubrinum; qRT-PCR