title: Gene-Centered Yeast One-Hybrid Assays.
abstract: An important question when studying gene regulation is which transcription factors (TFs) interact with which cis-regulatory elements, such as promoters and enhancers. Addressing this issue in complex multicellular organisms is challenging as several hundreds of TFs and thousands of regulatory elements must be considered in the context of different tissues and physiological conditions. Yeast one-hybrid (Y1H) assays provide a powerful "gene-centered" method to identify the TFs that can bind a DNA sequence of interest. In this introduction, we describe the basic principles of the Y1H assay and its advantages and disadvantages and briefly discuss how it is complementary to "TF-centered" methods that identify protein-DNA interactions for a known protein of interest.
authors.affiliation: Program in Systems Biology, University of Massachusetts Medical School, Worcester, Massachusetts 01605.
authors.forename: Juan I
authors.initials: JI
authors.lastname: Fuxman Bass
authors.affiliation: Program in Systems Biology, University of Massachusetts Medical School, Worcester, Massachusetts 01605.
authors.forename: John S
authors.initials: JS
authors.lastname: Reece-Hoyes
authors.affiliation: Program in Systems Biology, University of Massachusetts Medical School, Worcester, Massachusetts 01605.
authors.forename: Albertha J M
authors.initials: AJ
authors.lastname: Walhout
mesh_terms: D004271:DNA, Fungal / Q000378:metabolism; D015966:Gene Expression Regulation, Fungal; D011485:Protein Binding; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D014157:Transcription Factors / Q000378:metabolism; D020798:Two-Hybrid System Techniques
chemical_list: D004271:DNA, Fungal; D014157:Transcription Factors