title: Monolayers of immunoglobulin G on polystyrene microparticles and their interactions with human serum albumin.
abstract: The adsorption of mouse monoclonal immunoglobulin G (IgG) on negatively charged polystyrene microparticles was studied by the laser Doppler velocimetry (LDV) electrophoretic mobility measurements. The dependence of the electrophoretic mobility of microparticles on the IgG concentration in the suspension was determined for different ionic strengths and pHs (3.5, 7.4). The increase in the electrophoretic mobility was quantitatively interpreted in terms of the 3D electrokinetic model. The maximum coverage of IgG on latex was determined by the depletion method aided by AFM imaging. It was shown by monitoring the electrophoretic mobility and hydrodynamic dimeter of IgG covered microparticles over prolonged time periods that IgG adsorption was irreversible. The acid-base properties of the IgG monolayers were also determined in pH cycling experiments. It was also confirmed that the adsorption of human serum albumin (HSA) on saturated IgG monolayers, often referred to as blocking, was negligible at pH 7.4.
authors.affiliation: Jerzy Haber Institute of Catalysis and Surface Chemistry, Polish Academy of Science, Niezapominajek 8, PL-30239 Krakow, Poland. Electronic address: ncdabrow@cyf-kr.edu.pl.
authors.forename: Paulina
authors.initials: P
authors.lastname: Żeliszewska
authors.affiliation: Jerzy Haber Institute of Catalysis and Surface Chemistry, Polish Academy of Science, Niezapominajek 8, PL-30239 Krakow, Poland.
authors.forename: Monika
authors.initials: M
authors.lastname: Wasilewska
authors.affiliation: Jerzy Haber Institute of Catalysis and Surface Chemistry, Polish Academy of Science, Niezapominajek 8, PL-30239 Krakow, Poland.
authors.forename: Zbigniew
authors.initials: Z
authors.lastname: Adamczyk
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000737:chemistry; D006801:Humans; D006863:Hydrogen-Ion Concentration; D055767:Immobilized Proteins / Q000737:chemistry*; D007074:Immunoglobulin G / Q000737:chemistry*; D051379:Mice; D009994:Osmolar Concentration; D011137:Polystyrenes / Q000737:chemistry*; D012709:Serum Albumin / Q000737:chemistry*; D013499:Surface Properties
chemical_list: D000911:Antibodies, Monoclonal; D055767:Immobilized Proteins; D007074:Immunoglobulin G; D011137:Polystyrenes; D012709:Serum Albumin
keywords: Electrophoretic mobility of IgG covered polystyrene particles; HSA adsorption on IgG monolayers; IgG adsorption on microparticles; Monolayers of IgG on polystyrene particles; Stability of IgG monolayers on polystyrene particles; Zeta potential of IgG covered polystyrene particles