title: Sensitive immunoassay-based detection of Vibrio parahaemolyticus using capture and labeling particles in a stationary liquid phase lab-on-a-chip.
abstract: In the present study, a method was developed for detection of Vibrio parahaemolyticus based on a stationary liquid phase lab-on-a-chip (SLP LOC). The present SLP LOC comprises a sample chamber, washing chamber, and detection chamber connected by two channels. The method utilizes two types of particles: capture particles (CPs), which are magnetic nanoparticles functionalized with antibody; and labeling particles (LPs), which are silica nanoparticles functionalized with horseradish peroxidase and antibody. Samples were added to the sample chamber with CPs and LPs, forming a CP-bacteria-LP complex, and the complex was transported to the detection chamber containing chromogenic substrate solution. The method allowed the detection of V. parahaemolyticus in the range of 101-105cfu within 45min. Additionally, contamination of oyster samples with V. parahaemolyticus was detected within 2.5h, including 2h of culturing. The present method has the advantage of being highly rapid and facile, and enabling the detection of bacteria with high sensitivity. Moreover, the LOC and LOC processing device used in this method possess simple structures, making the detection process economical and allowing miniaturization. Therefore, the present SLP LOC detection method is potentially useful for in situ determination of food safety.
authors.affiliation: Department of Chemistry, Gangneung-Wonju National University, Gangneung 210-702, Republic of Korea.
authors.forename: Byunghee
authors.initials: B
authors.lastname: Park
authors.affiliation: Department of Chemistry, Gangneung-Wonju National University, Gangneung 210-702, Republic of Korea. Electronic address: sjchoi@gwnu.ac.kr.
authors.forename: Suk-Jung
authors.initials: SJ
authors.lastname: Choi
mesh_terms: D000818:Animals; D055910:Antibodies, Immobilized / Q000737:chemistry*; D015374:Biosensing Techniques / Q000295:instrumentation; D004867:Equipment Design; D005504:Food Analysis / Q000295:instrumentation*; D005506:Food Contamination / Q000032:analysis*; D006801:Humans; D007118:Immunoassay / Q000295:instrumentation*; D056656:Lab-On-A-Chip Devices; D057230:Limit of Detection; D059346:Magnets / Q000737:chemistry*; D010125:Ostreidae / Q000382:microbiology; D014735:Vibrio Infections / Q000382:microbiology; D014736:Vibrio parahaemolyticus / Q000302:isolation & purification*
chemical_list: D055910:Antibodies, Immobilized
keywords: Foodborne pathogens; Immunoassay; Lab-on-a-chip; Stationary liquid phase; Vibrio parahaemolyticus