title: Effect of suction pressures on cell yield and functionality of the adipose-derived stromal vascular fraction.
abstract: OBJECTIVE
  The present study was performed to explore the effect of suction pressures on the cell yield of adipose-derived stromal vascular fraction (SVF) and the functionality of adipose-derived stem cells (ADSCs), situated in the SVF, and to develop optimal parameters of harvesting SVF for clinical use.
  
  
  METHODS
  Adipose tissue was harvested from the lower abdomen of 10 patients by suction-assisted lipoplasty. Suction pressure was either -30 ± 5 kPa or -55 ± 5 kPa. The aspirated samples were subjected to macroscopic observation to verify the adipose particle size and cytological analysis to detect the cell yield and functionality of the SVF harvested.
  
  
  RESULTS
  Adipose tissue harvested at -30 ± 5 kPa appeared to have smaller particle sizes and less blood red cells than that harvested at -55 ± 5 kPa. Cell counts revealed that the cell number of the SVF obtained at -30 ± 5 kPa was more than 2-fold higher than that obtained at -55 ± 5 kPa. Cell growth at passages 1 and 2 was faster at -30 ± 5 kPa than that at -55 ± 5 kPa. The secretion of basic fibroblast growth factor and vascular endothelial growth factor as well as the capacity for adipogenic differentiation of the cultured cells at passages 1-3 were higher at -30 ± 5 kPa than those at -55 ± 5 kPa. There was no difference in the expression of the phenotypic markers between the two groups.
  
  
  CONCLUSIONS
  Our data indicate that the pressure for harvesting adipose tissue affects the yield and viability of the SVF. A lower suction pressure is beneficial to harvesting the SVF for clinical use.
authors.affiliation: Department of Plastic Surgery, The First Affiliated Hospital of Jinan University, Key Laboratory of Regenerative Medicine, Ministry of Education, Huangpu West Road 613, Guangzhou, Guangdong 510630, China.
authors.forename: Yuan-Wen
authors.initials: YW
authors.lastname: Chen
authors.affiliation: Department of Plastic Surgery, The First Affiliated Hospital of Jinan University, Key Laboratory of Regenerative Medicine, Ministry of Education, Huangpu West Road 613, Guangzhou, Guangdong 510630, China.
authors.forename: Jing-Ru
authors.initials: JR
authors.lastname: Wang
authors.affiliation: Department of Plastic Surgery, The First Affiliated Hospital of Jinan University, Key Laboratory of Regenerative Medicine, Ministry of Education, Huangpu West Road 613, Guangzhou, Guangdong 510630, China.
authors.forename: Xuan
authors.initials: X
authors.lastname: Liao
authors.affiliation: Department of Plastic Surgery, The First Affiliated Hospital of Jinan University, Key Laboratory of Regenerative Medicine, Ministry of Education, Huangpu West Road 613, Guangzhou, Guangdong 510630, China.
authors.forename: Sheng-Hong
authors.initials: SH
authors.lastname: Li
authors.affiliation: Department of Plastic Surgery, The First Affiliated Hospital of Jinan University, Key Laboratory of Regenerative Medicine, Ministry of Education, Huangpu West Road 613, Guangzhou, Guangdong 510630, China.
authors.forename: Li-Ling
authors.initials: LL
authors.lastname: Xiao
authors.affiliation: Department of Plastic Surgery, Guangzhou General Hospital of Guangzhou Military Command, Liuhua Road 111, Guangzhou, Guangdong 510010, China.
authors.forename: Biao
authors.initials: B
authors.lastname: Cheng
authors.affiliation: Department of Plastic Surgery, The First Affiliated Hospital of Jinan University, Key Laboratory of Regenerative Medicine, Ministry of Education, Huangpu West Road 613, Guangzhou, Guangdong 510630, China.
authors.forename: Guang-Hui
authors.initials: GH
authors.lastname: Xie
authors.affiliation: Department of Plastic Surgery, Changhai Hospital, The Second Military Medical University, Changhai Road 174, Shanghai 200433, China.
authors.forename: Jian-Xing
authors.initials: JX
authors.lastname: Song
authors.affiliation: Department of Plastic Surgery, The First Affiliated Hospital of Jinan University, Key Laboratory of Regenerative Medicine, Ministry of Education, Huangpu West Road 613, Guangzhou, Guangdong 510630, China. Electronic address: liuhongwei0521@hotmail.com.
authors.forename: Hong-Wei
authors.initials: HW
authors.lastname: Liu
mesh_terms: D017667:Adipocytes / Q000166:cytology; D000273:Adipose Tissue / Q000166:cytology*; D000328:Adult; D002454:Cell Differentiation; D049109:Cell Proliferation; D002478:Cells, Cultured; D005260:Female; D006801:Humans; D015187:Lipectomy / Q000379:methods*; D008875:Middle Aged; D011312:Pressure; D017154:Stromal Cells / Q000166:cytology*; D055815:Young Adult
keywords: Adipose tissue; Adipose-derived stem cells; Fat grafting; Liposuction; Stromal vascular fraction