title: [PRKAR1α expression in non-small cell lung cancer and  its clinicopathologic significance].
abstract: To evaluate the expression of cAMP-dependent protein kinase type I-alpha regulatory subunit (PRKAR1α) in non-small cell lung cancer (NSCLC) and its correlation with clinicopathological features.  Methods: PRKAR1α expressions in 79 NSCLC patients and matched adjacent non-carcinoma tissues were analyzed by using qRT-PCR and immunohistochemistry.  Results: The negative rates of PRKAR1α protein in NSCLC, lung squamous cell carcinoma (SCL) and lung adenocarcinoma (ACL) were 58.2%, 77.8%, 32.4%, respectively. Compared to the matched adjacent non-carcinoma tissues, there were significant differences in levels of PRKAR1α mRNA and protein in ACL (P<0.05), but not in SCL and overall NSCLC (P>0.05). The expression of PRKAR1α protein was positively correlated with histological type, TNM stage, and lymph node metastasis (P<0.05). Tumor size and histogenesis differentiation were not related to the decreased PRKAR1α (P>0.05).  Conclusion: Low expression of PRKAR1α in ACL might be involved in the pathogenesis, which might serve as a novel diagnostic candidate.
authors.affiliation: Department of Thoracic Surgery, Xiangya Hospital, Central South University, Changsha 410008, China.
authors.forename: Shaoqiang
authors.initials: S
authors.lastname: Wang
authors.affiliation: Department of Thoracic Surgery, Xiangya Hospital, Central South University, Changsha 410008, China.
authors.forename: Yuanda
authors.initials: Y
authors.lastname: Cheng
authors.affiliation: Department of Thoracic Surgery, Xiangya Hospital, Central South University, Changsha 410008, China.
authors.forename: Zhiwei
authors.initials: Z
authors.lastname: He
authors.affiliation: Department of Thoracic Surgery, Xiangya Hospital, Central South University, Changsha 410008, China.
authors.forename: Wolong
authors.initials: W
authors.lastname: Zhou
authors.affiliation: Department of Thoracic Surgery, Xiangya Hospital, Central South University, Changsha 410008, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Gao
authors.affiliation: Institute of Medical Sciences, Xiangya Hospital, Central South University, Changsha 410008, China.
authors.forename: Chaojun
authors.initials: C
authors.lastname: Duan
authors.affiliation: Department of Thoracic Surgery, Xiangya Hospital, Central South University, Changsha 410008, China.
authors.forename: Chunfang
authors.initials: C
authors.lastname: Zhang
mesh_terms: D000230:Adenocarcinoma / Q000737:chemistry* / Q000145:classification / Q000235:genetics; D000077192:Adenocarcinoma of Lung; D014408:Biomarkers, Tumor; D002289:Carcinoma, Non-Small-Cell Lung / Q000737:chemistry* / Q000235:genetics; D002294:Carcinoma, Squamous Cell / Q000737:chemistry* / Q000235:genetics; D054756:Cyclic AMP-Dependent Protein Kinase RIalpha Subunit / Q000502:physiology*; D005260:Female; D020869:Gene Expression Profiling; D006801:Humans; D007150:Immunohistochemistry; D008175:Lung Neoplasms / Q000737:chemistry* / Q000145:classification / Q000235:genetics; D008207:Lymphatic Metastasis / Q000235:genetics; D008297:Male; D009367:Neoplasm Staging; D012333:RNA, Messenger
chemical_list: D014408:Biomarkers, Tumor; D054756:Cyclic AMP-Dependent Protein Kinase RIalpha Subunit; C517834:PRKAR1A protein, human; D012333:RNA, Messenger