title: Discovery and identification of potential biomarkers for alcohol-induced oxidative stress based on cellular metabolomics.
abstract: Biomarkers involved in alcohol-induced oxidative stress play an important role in alcoholic liver disease prevention and diagnosis. Alcohol-induced oxidative stress in human liver L-02 cells was used to discover the potential biomarkers. Metabolites from L-02 cells induced by alcohol were measured by high-performance liquid chromatography and mass spectrometry. Fourteen metabolites that allowed discrimination between control and model groups were discovered by multivariate statistical data analysis (i.e. principal components analysis, orthogonal partial least-squares discriminate analysis). Based on the retention time, UV spectrum and LC-MS findings of the samples and compared with the authentic standards, eight biomarkers involved in alcohol-induced oxidative stress, namely, malic acid, oxidized glutathione, γ-glutamyl-cysteinyl-glycine, adenosine triphosphate, phenylalanine, adenosine monophosphate, nitrotyrosine and tryptophan, were identified. These biomarkers offered important targets for disease diagnosis and other researches.
authors.affiliation: Institute of Nutrition and Food Hygiene, School of Public Health, Lanzhou University, Lanzhou, China.|Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou, China.|Center of Resource Chemical and New Material, Qingdao, China.
authors.forename: Qingping
authors.initials: Q
authors.lastname: Hu
authors.affiliation: Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou, China.|Center of Resource Chemical and New Material, Qingdao, China.
authors.forename: Jianteng
authors.initials: J
authors.lastname: Wei
authors.affiliation: Institute of Nutrition and Food Hygiene, School of Public Health, Lanzhou University, Lanzhou, China.
authors.forename: Yewei
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Institute of Nutrition and Food Hygiene, School of Public Health, Lanzhou University, Lanzhou, China.
authors.forename: Xiulan
authors.initials: X
authors.lastname: Fei
authors.affiliation: Institute of Nutrition and Food Hygiene, School of Public Health, Lanzhou University, Lanzhou, China.
authors.forename: Yuwei
authors.initials: Y
authors.lastname: Hao
authors.affiliation: Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou, China.|Center of Resource Chemical and New Material, Qingdao, China.
authors.forename: Dong
authors.initials: D
authors.lastname: Pei
authors.affiliation: Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou, China.|Center of Resource Chemical and New Material, Qingdao, China.
authors.forename: Duolong
authors.initials: D
authors.lastname: Di
mesh_terms: D015415:Biomarkers / Q000378:metabolism*; D002460:Cell Line; D002851:Chromatography, High Pressure Liquid; D000431:Ethanol / Q000494:pharmacology*; D006801:Humans; D013058:Mass Spectrometry; D055432:Metabolomics; D018384:Oxidative Stress / Q000187:drug effects*; D017382:Reactive Oxygen Species / Q000378:metabolism
chemical_list: D015415:Biomarkers; D017382:Reactive Oxygen Species; D000431:Ethanol
keywords: alcohol-induced oxidative stress; biomarkers; human liver L-02 cells; orthogonal partial least-squares discriminate analysis; principal components analysis