title: The sequence spectrum of frameshift reversions obtained with a novel adaptive mutation assay inSaccharomyces cerevisiae.
abstract: Research on the mechanisms of adaptive mutagenesis in resting,i.e.non-replicating cells relies on appropriate mutation assays. Here we provide a novel procedure for the detection of frameshift-reverting mutations in yeast. Proliferation of non-reverted cells in this assay is suppressed by the lack of a fermentable carbon source. The test allele was constructed in a way that the reversions mimic microsatellite instability, a condition often found in cancer cells. We show the cell numbers during these starvation conditions and provide a DNA sequence spectrum of a representative set of revertants. The data in this article support the publication "Glucose starvation as a selective tool for the study of adaptive mutations inSaccharomyces cerevisiae" (Heidenreich and Steinboeck, 2016) [1].
authors.affiliation: Institute of Cancer Research, Department of Medicine I, Medical University of Vienna, Borschkegasse 8a, A-1090 Vienna, Austria.
authors.forename: Erich
authors.initials: E
authors.lastname: Heidenreich
authors.affiliation: Institute of Cancer Research, Department of Medicine I, Medical University of Vienna, Borschkegasse 8a, A-1090 Vienna, Austria.
authors.forename: Ferdinand
authors.initials: F
authors.lastname: Steinboeck
keywords: Adaptive mutation; Selection-induced; Spontaneous mutagenesis; Stress-induced