title: Electrophysiological Properties of CA1 Pyramidal Neurons along the Longitudinal Axis of the Mouse Hippocampus.
abstract: Evidence for different physiological properties along the hippocampal longitudinal axis is emerging. Here, we examined the electrophysiological features of neurons at different dorso-ventral sites of the mouse CA1 hippocampal region. Cell position was defined with respect to longitudinal coordinates of each slice. We measured variations in neuronal excitability, subthreshold membrane properties and neurotransmitter responses along the longitudinal axis. We found that (i) pyramidal cells of the dorsal hippocampus (DH) were less excitable than those of the ventral hippocampus (VH). Resting Membrane Potential (RMP) was more hyperpolarized and somatic Input Resistance (Ri) was lower in DH compared to VH. (ii) The Paired-pulse ratio (PPR) of focally induced synaptic responses was systematically reduced from the DH to the VH; (iii) Long-term-potentiation was most pronounced in the DH and fell gradually in the intermediate hippocampus and in the VH; (iv) the frequency of miniature GABAergic events was higher in the VH than in the DH; (v) the PPR of evoked inhibitory post-synaptic current (IPSC) was higher in the DH than in the VH. These findings indicate an increased probability of both GABA and glutamate release and a reduced plasticity in the ventral compared to more dorsal regions of the hippocampus.
authors.affiliation: Pasteur Institute Rome-Department of Physiology and Pharmacology, Sapienza University of Rome, Italy.|Inserm U1127, CNRS UMR7225, Sorbonne Universités, UPMC UMR S1127, Institut du Cerveau et de la Moelle épinière, Paris 75013, France.
authors.forename: Giampaolo
authors.initials: G
authors.lastname: Milior
authors.affiliation: Pasteur Institute Rome-Department of Physiology and Pharmacology, Sapienza University of Rome, Italy.
authors.forename: Maria Amalia
authors.initials: MA
authors.lastname: Di Castro
authors.affiliation: Pasteur Institute Rome-Department of Physiology and Pharmacology, Sapienza University of Rome, Italy.
authors.forename: Livio Pepe'
authors.initials: LP
authors.lastname: Sciarria
authors.affiliation: Pasteur Institute Rome-Department of Physiology and Pharmacology, Sapienza University of Rome, Italy.
authors.forename: Stefano
authors.initials: S
authors.lastname: Garofalo
authors.affiliation: Section of Behavioural Neurosciences, Department of Cell Biology and Neurosciences, Istituto Superiore di Sanità, Rome, Italy.
authors.forename: Igor
authors.initials: I
authors.lastname: Branchi
authors.affiliation: Pasteur Institute Rome-Department of Physiology and Pharmacology, Sapienza University of Rome, Italy.|IRCCS Neuromed, Pozzilli, IS, Italy.
authors.forename: Davide
authors.initials: D
authors.lastname: Ragozzino
authors.affiliation: Pasteur Institute Rome-Department of Physiology and Pharmacology, Sapienza University of Rome, Italy.|IRCCS Neuromed, Pozzilli, IS, Italy.
authors.forename: Cristina
authors.initials: C
authors.lastname: Limatola
authors.affiliation: Pasteur Institute Rome-Department of Physiology and Pharmacology, Sapienza University of Rome, Italy.
authors.forename: Laura
authors.initials: L
authors.lastname: Maggi
mesh_terms: D000200:Action Potentials; D000818:Animals; D056547:CA1 Region, Hippocampal / Q000502:physiology; D004594:Electrophysiology / Q000379:methods*; D006624:Hippocampus / Q000502:physiology*; D008297:Male; D008810:Mice, Inbred C57BL; D009473:Neuronal Plasticity / Q000502:physiology; D009474:Neurons / Q000502:physiology; D009924:Organ Culture Techniques / Q000379:methods; D005680:gamma-Aminobutyric Acid / Q000378:metabolism
chemical_list: D005680:gamma-Aminobutyric Acid