title: Proteomic profiles of thyroid tumors by mass spectrometry-imaging on tissue microarrays.
abstract: The current study proposes the successful use of a mass spectrometry-imaging technology that explores the composition of biomolecules and their spatial distribution directly on-tissue to differentially classify benign and malignant cases, as well as different histotypes. To identify new specific markers, we investigated with this technology a wide histological Tissue Microarray (TMA)-based thyroid lesion series. Results showed specific protein signatures for malignant and benign specimens and allowed to build clusters comprising several proteins with discriminant capabilities. Among them, FINC, ACTB1, LMNA, HSP7C and KAD1 were identified by LC-ESI-MS/MS and found up-expressed in malignant lesions. These findings represent the opening of further investigations for their translation into clinical practice, e.g. for setting up new immunohistochemical stainings, and for a better understanding of thyroid lesions. This article is part of a Special Issue entitled: MALDI Imaging, edited by Dr. Corinna Henkel and Prof. Peter Hoffmann.
authors.affiliation: Department of Medicine and Surgery, University of Milano-Bicocca, Milan, Italy.
authors.forename: Manuel
authors.initials: M
authors.lastname: Galli
authors.affiliation: Department of Medicine and Surgery, University of Milano-Bicocca, Milan, Italy.
authors.forename: Fabio
authors.initials: F
authors.lastname: Pagni
authors.affiliation: Department of Medicine and Surgery, University of Milano-Bicocca, Milan, Italy.
authors.forename: Gabriele
authors.initials: G
authors.lastname: De Sio
authors.affiliation: Department of Medicine and Surgery, University of Milano-Bicocca, Milan, Italy.
authors.forename: Andrew
authors.initials: A
authors.lastname: Smith
authors.affiliation: Department of Medicine and Surgery, University of Milano-Bicocca, Milan, Italy.
authors.forename: Clizia
authors.initials: C
authors.lastname: Chinello
authors.affiliation: Department of Medicine and Surgery, University of Milano-Bicocca, Milan, Italy.
authors.forename: Martina
authors.initials: M
authors.lastname: Stella
authors.affiliation: Department of Medicine and Surgery, University of Milano-Bicocca, Milan, Italy.
authors.forename: Vincenzo
authors.initials: V
authors.lastname: L'Imperio
authors.affiliation: Department of Medicine and Surgery, University of Milano-Bicocca, Milan, Italy.
authors.forename: Marco
authors.initials: M
authors.lastname: Manzoni
authors.affiliation: Department of Surgery, Hospital San Gerardo, Monza Brianza, Italy.
authors.forename: Mattia
authors.initials: M
authors.lastname: Garancini
authors.affiliation: Department of Surgery, Hospital San Gerardo, Monza Brianza, Italy.
authors.forename: Diego
authors.initials: D
authors.lastname: Massimini
authors.affiliation: Department of Medicine and Surgery, University of Milano-Bicocca, Milan, Italy.
authors.forename: Niccolò
authors.initials: N
authors.lastname: Mosele
authors.affiliation: Department of Informatics, Systems and Communication, University of Milano-Bicocca, Milan, Italy.
authors.forename: Giancarlo
authors.initials: G
authors.lastname: Mauri
authors.affiliation: Department of Informatics, Systems and Communication, University of Milano-Bicocca, Milan, Italy.
authors.forename: Italo
authors.initials: I
authors.lastname: Zoppis
authors.affiliation: Department of Medicine and Surgery, University of Milano-Bicocca, Milan, Italy. Electronic address: Fulvio.Magni@unimib.it.
authors.forename: Fulvio
authors.initials: F
authors.lastname: Magni
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000378:metabolism; D002853:Chromatography, Liquid / Q000379:methods; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D020543:Proteome / Q000378:metabolism*; D040901:Proteomics / Q000379:methods; D053719:Tandem Mass Spectrometry / Q000379:methods; D013961:Thyroid Gland / Q000378:metabolism / Q000502:physiology; D013964:Thyroid Neoplasms / Q000378:metabolism* / Q000473:pathology*; D055815:Young Adult
chemical_list: D014408:Biomarkers, Tumor; D020543:Proteome
keywords: Cancer; Mass spectrometry imaging; Proteomics; Thyroid; Tissue microarray