title: Gene expression and pathway analysis ofCTNNB1in cancer and stem cells.
abstract: OBJECTIVE
  To investigate β-catenin (CTNNB1) signaling in cancer and stem cells, the gene expression and pathway were analyzed using bioinformatics.
  
  
  METHODS
  The expression of the catenin β 1 (CTNNB1) gene, which codes for β-catenin, was analyzed in mesenchymal stem cells (MSCs) and gastric cancer (GC) cells. Beta-catenin signaling and the mutation of related proteins were also analyzed using the cBioPortal for Cancer Genomics and HOMology modeling of Complex Structure (HOMCOS) databases.
  
  
  RESULTS
  The expression of theCTNNB1gene was up-regulated in GC cells compared to MSCs. The expression of EPH receptor A8 (EPHA8), synovial sarcoma translocation chromosome 18 (SS18), interactor of little elongation complex ELL subunit 1 (ICE1), patched 1 (PTCH1), mutS homolog 3 (MSH3) and caspase recruitment domain family member 11 (CARD11) were also shown to be altered in GC cells in the cBioPortal for Cancer Genomics analysis. 3D complex structures were reported for E-cadherin 1 (CDH1), lymphoid enhancer binding factor 1 (LEF1), transcription factor 7 like 2 (TCF7L2) and adenomatous polyposis coli protein (APC) with β-catenin.
  
  
  CONCLUSIONS
  The results indicate that the epithelial-mesenchymal transition (EMT)-related geneCTNNB1plays an important role in the regulation of stem cell pluripotency and cancer signaling.
authors.affiliation: Shihori Tanabe, Division of Risk Assessment, National Institute of Health Sciences, Tokyo 158-8501, Japan.
authors.forename: Shihori
authors.initials: S
authors.lastname: Tanabe
authors.affiliation: Shihori Tanabe, Division of Risk Assessment, National Institute of Health Sciences, Tokyo 158-8501, Japan.
authors.forename: Takeshi
authors.initials: T
authors.lastname: Kawabata
authors.affiliation: Shihori Tanabe, Division of Risk Assessment, National Institute of Health Sciences, Tokyo 158-8501, Japan.
authors.forename: Kazuhiko
authors.initials: K
authors.lastname: Aoyagi
authors.affiliation: Shihori Tanabe, Division of Risk Assessment, National Institute of Health Sciences, Tokyo 158-8501, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Yokozaki
authors.affiliation: Shihori Tanabe, Division of Risk Assessment, National Institute of Health Sciences, Tokyo 158-8501, Japan.
authors.forename: Hiroki
authors.initials: H
authors.lastname: Sasaki
keywords: CTNNB1; Epithelial-mesenchymal transition; Mesenchymal stem cell; Stem cell; β-catenin