title: Polymorphisms in ten candidate genes are associated with conformational and locomotive traits in Spanish Purebred horses.
abstract: The Spanish Purebred horses, also known as Andalusian horses, compete to the highest standards in international dressage events. Gait and conformation could be used as early selection criteria to detect young horses with promising dressage ability. Although the genetic background of equine size variation has been recently uncovered, the genetic basis of horse conformational and locomotive traits is not known, hampered by the complex genetic architecture underlying quantitative traits and the lack of phenotypic data. The aim of this study was to validate the loci associated with size in 144 Spanish Purebred horses, and to seek novel associations between loci previously associated with the development of osteochondrosis (OC) lesions and 20 conformational and locomotive traits. Ten loci were associated with different conformational and locomotive traits (LCORL/NCAPG, HMGA2, USP31, MECR, COL24A1, MGP, FAM184B, PTH1R, KLF3 and SGK1), and the LCORL/NCAPG association with size in the Spanish Purebred horse was validated. Except for HMGA2, all polymorphisms seem to influence both the prevalence of OC lesions and morphological characters, supporting the link between conformation and OC. Also, the implication of most genes in either immune and inflammatory responses and cellular growth, or ossification processes, reinforces the role that these mechanisms have in the aetiology of OC, as well as their reflection on the general conformation of the individual. These polymorphisms could be used in marker-assisted selection (MAS) programmes to improve desirable conformational traits, but taking into account their possible detrimental effect on OC prevalence.
authors.affiliation: Departamento de Producción Animal, Facultad de Veterinaria, Universidad Complutense, Madrid, 28040, Spain. nsevane@ucm.es.
authors.forename: Natalia
authors.identifier: http://orcid.org/0000-0003-4766-6291
authors.initials: N
authors.lastname: Sevane
authors.affiliation: Departamento de Producción Animal, Facultad de Veterinaria, Universidad Complutense, Madrid, 28040, Spain.
authors.forename: Susana
authors.initials: S
authors.lastname: Dunner
authors.affiliation: Traumatología Equina, El Boalo, Madrid, 28413, Spain.
authors.forename: Ana
authors.initials: A
authors.lastname: Boado
authors.affiliation: Departamento de Producción Animal, Facultad de Veterinaria, Universidad Complutense, Madrid, 28040, Spain.
authors.forename: Javier
authors.initials: J
authors.lastname: Cañon
mesh_terms: D000818:Animals; D001947:Breeding; D005684:Gait / Q000235:genetics*; D056426:Genetic Loci; D005838:Genotype; D006734:Horse Diseases / Q000235:genetics*; D006736:Horses / Q000235:genetics*; D016014:Linear Models; D055034:Osteochondrosis / Q000235:genetics / Q000662:veterinary*; D010641:Phenotype; D020641:Polymorphism, Single Nucleotide; D013030:Spain
keywords: Association; Equus caballus; Morphology; SNP; Single nucleotide polymorphism