title: Encapsulation of β-Sitosterol in Polyurethane by Sol-Gel Electrospinning.
abstract: Pristine β-sitosterol or in combination with other phytosterols is utilized in an array of enriched commercial foods. Considering the presence of β-sitosterol in different functional foods and its potential role in prevention and cure of neurodegenerative diseases, the aims of our investigation were to encapsulate β-sitosterol in nanofibers and to estimate influence of β-sitosterol on proliferation of fibroblasts. Electrospun nanofibers have widely been used as scaffolds to mimic natural extracellular matrix. Herein, our group for the first time establishes an innovative scaffold based on β-sitosterol and polyurethane using electrospinning. β-Sitosterol promotes epithelialization and possesses anti-oxidant and anti-inflammatory activities, whereas polyurethane, besides possessing biomedical uses, also enhances epithelial growth. We optimized the concentration (5%) of β-sitosterol in polyurethane to obtain homogenous solution, which can be spun without difficulty for the synthesis of β-sitosterol amalgamated scaffold. The resulted twisted nanofibers have been characterized via scanning electron microscopy and Fourier transform infrared spectroscopy. The viability of cells on twisted scaffold was examined using NIH 3T3 fibroblasts as model cell line. Incorporation of β-sitosterol in polyurethane changed the structure and size of nanofibers, and the twisted scaffolds were non-cytotoxic. Thus, the twisted nanoribbons, which contain anti-inflammatory β-sitosterol, can be utilized as a promising future material, which will help to ease inflammation and also aid in wound healing. In conclusion, the outcome of the preliminary research evidently points out the potential of twisted scaffold in biomedical applications.
authors.affiliation: Department of Pharmacognosy, Pharmacy College, King Saud University, Riyadh, Kingdom of Saudi Arabia.
authors.forename: Musarat
authors.initials: M
authors.lastname: Amina
authors.affiliation: Department of Biology, Faculty of Science, Albaha University, Albaha, 1988, Kingdom of Saudi Arabia. touseefamna@gmail.com.|Department of Organic Material and Fiber Engineering, Chonbuk National University, Jeonju, 561-756, Republic of Korea. touseefamna@gmail.com.
authors.forename: Touseef
authors.initials: T
authors.lastname: Amna
authors.affiliation: Department of Pharmacognosy, Pharmacy College, King Saud University, Riyadh, Kingdom of Saudi Arabia.
authors.forename: Nawal
authors.initials: N
authors.lastname: Al-Musayeib
authors.affiliation: Department of Chemistry, Faculty of Science, Albaha University, Albaha, 1988, Kingdom of Saudi Arabia.
authors.forename: Sami A
authors.initials: SA
authors.lastname: Zabin
authors.affiliation: Department of Organic Material and Fiber Engineering, Chonbuk National University, Jeonju, 561-756, Republic of Korea.|Department of Chemistry, Faculty of Science, Albaha University, Albaha, 1988, Kingdom of Saudi Arabia.
authors.forename: M Shamshi
authors.initials: MS
authors.lastname: Hassan
authors.affiliation: Department of Organic Material and Fiber Engineering, Chonbuk National University, Jeonju, 561-756, Republic of Korea. mskhil@jbnu.ac.kr.
authors.forename: Myung-Seob
authors.initials: MS
authors.lastname: Khil
mesh_terms: D000818:Animals; D055664:Electrochemical Techniques; D008422:Materials Testing; D051379:Mice; D041681:NIH 3T3 Cells; D057139:Nanofibers / Q000737:chemistry*; D044367:Phase Transition; D011140:Polyurethanes / Q000737:chemistry*; D012855:Sitosterols / Q000737:chemistry*
chemical_list: D011140:Polyurethanes; D012855:Sitosterols; C025473:gamma-sitosterol
keywords: Anti-inflammatory; Nanoribbons; Polyurethane; Scaffold; Wound healing; β-Sitosterol