title: Biotransformation of lignan glycoside to its aglycone by Woodfordia fruticosa flowers: quantification of compounds using a validated HPTLC method.
abstract: BACKGROUND
  Saraca asoca Linn. (Caesalpiniaceae) is an important traditional remedy for gynaecological disorders and it contains lyoniside, an aryl tetralin lignan glycoside. The aglycone of lyoniside, lyoniresinol possesses structural similarity to enterolignan precursors which are established phytoestrogens.
  
  
  OBJECTIVE
  This work illustrates biotransformation of lyoniside to lyoniresinol using Woodfordia fruticosa Kurz. (Lythraceae) flowers and simultaneous quantification of lyoniside and lyoniresinol using a validated HPTLC method.
  
  
  METHODS
  The aqueous extract prepared from S. asoca bark was fermented using W. fruticosa flowers. The substrate and fermented product both were simultaneously analyzed using solvent system:toluene:ethyl acetate:formic acid (4:3:0.4) at 254 nm. The method was validated for specificity, accuracy, precision, linearity, sensitivity and robustness as per ICH guidelines.
  
  
  RESULTS
  The substrate showed the presence of lyoniside, however, it decreased as the fermentation proceeded. On 3rd day, lyoniresinol starts appearing in the medium. In 8 days duration most of the lyoniside converted to lyoniresinol. The developed method was specific for lyoniside and lyoniresinol. Lyoniside and lyoniresinol showed linearity in the range of 250-3000 and 500-2500 ng. The method was accurate as resulted in 99.84% and 99.83% recovery, respectively, for lyoniside and lyoniresinol.
  
  
  CONCLUSIONS
  Aryl tetralin lignan glycoside, lyoniside was successfully transformed into lyoniresinol using W. fruticosa flowers and their contents were simultaneously analyzed using developed validated HPTLC method.
authors.affiliation: a Department of Pharmacognosy and Phytochemistry , Jamia Hamdard , New Delhi , India.
authors.forename: Shikha
authors.initials: S
authors.lastname: Mishra
authors.affiliation: a Department of Pharmacognosy and Phytochemistry , Jamia Hamdard , New Delhi , India.
authors.forename: Vidhu
authors.initials: V
authors.lastname: Aeri
mesh_terms: D000840:Anisoles / Q000378:metabolism*; D001711:Biotransformation; D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000592:standards; D003720:Densitometry; D005285:Fermentation; D035264:Flowers / Q000378:metabolism*; D006027:Glycosides / Q000378:metabolism*; D017705:Lignans / Q000378:metabolism*; D016014:Linear Models; D009281:Naphthalenes / Q000378:metabolism*; D008517:Phytotherapy; D024301:Plant Bark; D010946:Plants, Medicinal; D012015:Reference Standards; D015203:Reproducibility of Results; D012855:Sitosterols / Q000378:metabolism*; D031565:Woodfordia / Q000378:metabolism*
chemical_list: D000840:Anisoles; D006027:Glycosides; D017705:Lignans; D009281:Naphthalenes; D012855:Sitosterols; C502379:lyoniresinol; C011015:lyoniside
keywords: Asokarista®; Lyoniside; NMR; lyoniresinol; validation