title: High maysin corn silk extract reduces body weight and fat deposition in C57BL/6J mice fed high-fat diets.
abstract: The study was performed to investigate the effects and mechanisms of action of high maysin corn silk extract on body weight and fat deposition in experimental animals.
  
  
  METHODS
  A total of 30 male C57BL/6J mice, 4-weeks-old, were purchased and divided into three groups by weight using a randomized block design. The normal-fat (NF) group received 7% fat (diet weight basis), the high-fat (HF) group received 25% fat and 0.5% cholesterol, and the high-fat corn silk (HFCS) group received high-fat diet and high maysin corn silk extract at 100 mg/kg body weight through daily oral administration. Body weight and body fat were measured, and mRNA expression levels of proteins involved in adipocyte differentiation, fat accumulation, fat synthesis, lipolysis, and fat oxidation in adipose tissue and the liver were measured.
  
  
  RESULTS
  After experimental diet intake for 8 weeks, body weight was significantly lower in the HFCS group compared to the HF group (P< 0.05), and kidney fat and epididymal fat pad weights were significantly lower in the HFCS group compared to the HF group (P< 0.05). In the HFCS group, CCAAT/enhancer binding protein-β, peroxisome proliferator-activated receptor-γ1 (PPAR-γ1), and PPAR-γ2 mRNA expression levels were significantly reduced (P< 0.05) in the epididymal fat pad, whereas cluster of differentiation 36, lipoprotein lipase, acetyl-CoA carboxylase-1, sterol regulatory element binding protein-1c, pyruvate dehydrogenase kinase, isozyme-4, glucose-6-phosphate dehydrogenase, and stearoyl-CoA desaturase-1 mRNA expression levels were significantly decreased in liver and adipose tissues (P< 0.05). In the HFCS group, mRNA expression levels of AMP-activated protein kinase, hormone-sensitive lipase, and carnitine palmitoyltransferase-1 were elevated (P< 0.05).
  
  
  CONCLUSIONS
  It can be concluded that high maysin corn silk extract inhibits expression of genes involved in adipocyte differentiation, fat accumulation, and fat synthesis as well as promotes expression of genes involved in lipolysis and fat oxidation, further inhibiting body fat accumulation and body weight elevation in experimental animals.
authors.affiliation: Department of Food Science and Nutrition, Dankook University, 152, Juljeon-ro, Suji-gu, Yonin-si, Gyeonggi 16890, Korea.
authors.forename: Eun Young
authors.initials: EY
authors.lastname: Lee
authors.affiliation: Crop Foundation Division National Institute of Crop Science, Jeonbuk 55365, Korea.
authors.forename: Sun Lim
authors.initials: SL
authors.lastname: Kim
authors.affiliation: Crop Foundation Division National Institute of Crop Science, Jeonbuk 55365, Korea.
authors.forename: Hyeon Jung
authors.initials: HJ
authors.lastname: Kang
authors.affiliation: Department of Food Engineering, Dankook University, Chungnam 31116, Korea.
authors.forename: Myung Hwan
authors.initials: MH
authors.lastname: Kim
authors.affiliation: Department of Food Science and Nutrition, Dankook University, 152, Juljeon-ro, Suji-gu, Yonin-si, Gyeonggi 16890, Korea.
authors.forename: Ae Wha
authors.initials: AW
authors.lastname: Ha
authors.affiliation: Department of Food Science and Nutrition, Dankook University, 152, Juljeon-ro, Suji-gu, Yonin-si, Gyeonggi 16890, Korea.
authors.forename: Woo Kyoung
authors.initials: WK
authors.lastname: Kim
keywords: Corn silk; adipocyte differentiation; body weight; fat synthesis; maysin