title: Fast and selective cell isolation from blood sample by microfiber fabric system with vacuum aspiration.
abstract: Since circulating tumor cells (CTCs) are tumor cells which are found in the blood of cancer patients, CTCs are potential tumor markers, so a rapid isolation of CTCs is desirable for clinical applications. In this paper, a three-dimensional polystyrene (PS) microfiber fabric with vacuum aspiration system was developed for capturing CTCs within a short time. Various microfiber fabrics with different diameters were prepared by the electrospinning method and optimized for contact frequency with cells. Vacuum aspiration utilizing these microfiber fabrics could filter all cells within seconds without mechanical damage. The microfiber fabric with immobilized anti-EpCAM antibodies was able to specifically capture MCF-7 cells that express EpCAM on their surfaces. The specificity of the system was confirmed by monitoring the ability to isolate MCF-7 cells from a mixture containing CCRF-CEM cells that do not express EpCAM. Furthermore, the selective capture ability of the microfiber was retained even when the microfiber was exposed to the whole blood of pigs spiked with MCF-7 cells. The specific cell capture ratio of the vacuum aspiration system utilizing microfiber fabric could be improved by increasing the thickness of the microfiber fabric through electrospinning time.
authors.affiliation: Department of Bioengineering, The University of Tokyo , Tokyo , Japan.
authors.forename: Takayuki
authors.initials: T
authors.lastname: Ueki
authors.affiliation: Department of Bioengineering, The University of Tokyo , Tokyo , Japan.
authors.forename: Akifumi
authors.initials: A
authors.lastname: Yoshihara
authors.affiliation: Department of Bioengineering, The University of Tokyo, Tokyo, Japan; Department of Immunology, Genetics and Pathology (IGP), Uppsala University, Uppsala, Sweden.
authors.forename: Yuji
authors.initials: Y
authors.lastname: Teramura
authors.affiliation: Department of Bioengineering, The University of Tokyo , Tokyo , Japan.
authors.forename: Madoka
authors.initials: M
authors.lastname: Takai
keywords: 102 Porous / Nanoporous / Nanostructured materials; 211 Scaffold / Tissue engineering/Drug delivery; 212 Surface and interfaces; 30 Bio-inspired and biomedical materials; Circulating tumor cells (CTCs); EpCAM; electrospun microfiber fabric; vacuum aspiration