title: Synthesis of silica-PAMAM dendrimer nanoparticles as promising carriers in Neuro blastoma cells.
abstract: Mesoporous silica carriers are emerging as therapeutic drug delivery systems. The objective of this study was to develop a formulation for synthesizing silica-PAMAM dendrimer hybrid nanoparticles with sol-gel technique. Subsequently, black carrot anthocyanins were encapsulated and investigated for their capability in terms of inhibiting the proliferative effects of neuroblastoma (Neuro 2A). In this context, particle size distributions were ascertained followed by thermal analysis (DSC), scanning electron microscopy and encapsulation efficiency. Subsequently, in vitro release kinetics was determined along with cytotoxicity of empty and anthocyanin doped hybrid nanoparticles. The lowest particle size was 134.8 nm with a zeta potential of +19.78 mV which enhanced electrostatic interaction with the cell membrane in the cytotoxicity analyses. As the anthocyanin content was totally released at the end of 6 days, the cytotoxicity was observed for 134 h, reaching an inhibition of 87.9%. On the other hand, Neuro 2A cells incubated with empty nanoparticles exhibited a high proliferation indicating that hybrid nanoparticles were not toxic to the cells and the inhibitory effect was associated with the anthocyanins.
authors.affiliation: Novel Fludic Technologies Group, Department of Bioengineering, Faculty of Engineering, Ege University, 35100 Bornova-Izmir, Turkey. Electronic address: ozlem.yesil.celiktas@ege.edu.tr.
authors.forename: Ozlem
authors.initials: O
authors.lastname: Yesil-Celiktas
authors.affiliation: Novel Fludic Technologies Group, Department of Bioengineering, Faculty of Engineering, Ege University, 35100 Bornova-Izmir, Turkey.
authors.forename: Cansu
authors.initials: C
authors.lastname: Pala
authors.affiliation: Department of Biopharmaceutics and Pharmacokinetics, Faculty of Pharmacy, Ege University, 35100 Bornova-Izmir, Turkey.
authors.forename: E Oyku
authors.initials: EO
authors.lastname: Cetin-Uyanikgil
authors.affiliation: Department of Biology, Science and Art Faculty, Kocaeli University, Kocaeli, Turkey.
authors.forename: Canan
authors.initials: C
authors.lastname: Sevimli-Gur
mesh_terms: D000872:Anthocyanins / Q000737:chemistry / Q000494:pharmacology*; D049109:Cell Proliferation / Q000187:drug effects*; D018552:Daucus carota / Q000737:chemistry; D050091:Dendrimers / Q000737:chemistry*; D004337:Drug Carriers; D016503:Drug Delivery Systems; D006801:Humans; D053758:Nanoparticles / Q000008:administration & dosage / Q000737:chemistry*; D009447:Neuroblastoma / Q000188:drug therapy / Q000473:pathology*; D012822:Silicon Dioxide / Q000737:chemistry*; D014407:Tumor Cells, Cultured
chemical_list: D000872:Anthocyanins; D050091:Dendrimers; D004337:Drug Carriers; C104700:PAMAM Starburst; D012822:Silicon Dioxide
keywords: Cytotoxicity; Dendrimer; In vitro release profile; Nanoparticle; Neuro 2A; Sol-gel