title: Peptidomic Approach to Developing ELISAs for the Determination of Bovine and Porcine Processed Animal Proteins in Feed for Farmed Animals.
abstract: The European Commission (EC) wants to reintroduce nonruminant processed animal proteins (PAPs) safely into the feed chain. This would involve replacing the current ban in feed with a species-to-species ban which, in the case of nonruminants, would only prohibit feeding them with proteins from the same species. To enforce such a provision, there is an urgent need for species-specific methods for detecting PAPs from several species in animal feed and in PAPs from other species. Currently, optical microscopy and the polymerase chain reaction are the officially accepted methods, but they have limitations, and alternative methods are needed. We have developed immunoassays using antibodies raised against targets which are not influenced by high temperature and pressure. These targets were identified in a previous study based on an experimental approach. One optimized competitive ELISA detects bovine PAPs at 2% in plant-derived feed. The detection capability demonstrated on blind samples shows a good correlation with mass spectrometry results.
authors.affiliation: CER Groupe, Health Department, Rue du Point du Jour 8, 6900 Marloie, Belgium.
authors.forename: Anne-Catherine
authors.initials: AC
authors.lastname: Huet
authors.affiliation: CER Groupe, Health Department, Rue du Point du Jour 8, 6900 Marloie, Belgium.
authors.forename: Caroline
authors.initials: C
authors.lastname: Charlier
authors.affiliation: CER Groupe, Health Department, Rue du Point du Jour 8, 6900 Marloie, Belgium.
authors.forename: Elise
authors.initials: E
authors.lastname: Deckers
authors.affiliation: URBC-Narilis, University of Namur , Rue de Bruxelles 61, 5000 Namur, Belgium.
authors.forename: Hélène
authors.initials: H
authors.lastname: Marbaix
authors.affiliation: URBC-Narilis, University of Namur , Rue de Bruxelles 61, 5000 Namur, Belgium.
authors.forename: Martine
authors.initials: M
authors.lastname: Raes
authors.affiliation: CRA-W, Walloon Agricultural Research Center, Biotechnology Department, Chaussée de Charleroi 234, 5030 Gembloux, Belgium.
authors.forename: Sergio
authors.initials: S
authors.lastname: Mauro
authors.affiliation: CER Groupe, Health Department, Rue du Point du Jour 8, 6900 Marloie, Belgium.
authors.forename: Philippe
authors.initials: P
authors.lastname: Delahaut
authors.affiliation: CER Groupe, Health Department, Rue du Point du Jour 8, 6900 Marloie, Belgium.
authors.forename: Nathalie
authors.initials: N
authors.lastname: Gillard
mesh_terms: D000821:Animal Feed / Q000032:analysis*; D000818:Animals; D002417:Cattle; D004032:Diet / Q000662:veterinary; D004044:Dietary Proteins / Q000008:administration & dosage / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay; D005506:Food Contamination / Q000032:analysis; D007118:Immunoassay; D016133:Polymerase Chain Reaction; D013045:Species Specificity; D013552:Swine
chemical_list: D004044:Dietary Proteins
keywords: PAPs; animal feed; immunoassay; peptide biomarker