title: Identification of Ochratoxin A-Producing Black Aspergilli from Grapes Using Loop-Mediated Isothermal Amplification (LAMP) Assays.
abstract: The loop-mediated isothermal amplification (LAMP) allows the rapid and specific amplification of target DNA under isothermal conditions without a prior DNA purification step. Moreover, successful amplifications can be directly evaluated through a color change of the reaction solutions. Here, we describe two LAMP assays for the detection of ochratoxin-A producing black aspergilli isolated from grapes. The two assays can detect DNA of OTA-producing black aspergilli following a very simple sample preparation and have the potential to significantly speed up the routine monitoring of these toxigenic molds in vineyards.
authors.affiliation: Institute for Food Sciences, Agroscope, Schwarzenburgstrasse 161, 3003, Bern, Switzerland. michelangelo.storari@agroscope.admin.ch.
authors.forename: Michelangelo
authors.initials: M
authors.lastname: Storari
authors.affiliation: Institute for Plant Production Sciences, Agroscope, Wädenswil, Switzerland.
authors.forename: Giovanni A L
authors.initials: GA
authors.lastname: Broggini
mesh_terms: D001230:Aspergillus / Q000145:classification / Q000235:genetics* / Q000378:metabolism*; D004271:DNA, Fungal; D021141:Nucleic Acid Amplification Techniques; D009793:Ochratoxins / Q000096:biosynthesis*; D027843:Vitis / Q000382:microbiology*
chemical_list: D004271:DNA, Fungal; D009793:Ochratoxins; C025589:ochratoxin A
keywords: Climate change; Hydroxynaphthol blue; Mycotoxins; Polyketide synthase; Wine