title: New Fluorescent Macrolide Derivatives for Studying Interactions of Antibiotics and Their Analogs with the Ribosomal Exit Tunnel.
abstract: Novel fluorescent derivatives of macrolide antibiotics related to tylosin bearing rhodamine, fluorescein, Alexa Fluor 488, BODIPY FL, and nitrobenzoxadiazole (NBD) residues were synthesized. The formation of complexes of these compounds with 70S E. coli ribosomes was studied by measuring the fluorescence polarization depending on the ribosome amount at constant concentration of the fluorescent substance. With the synthesized fluorescent tylosin derivatives, the dissociation constants for ribosome complexes with several known antibiotics and macrolide analogs previously obtained were determined. It was found that the fluorescent tylosin derivatives containing BODIPY FL and NBD groups could be used to screen the binding of novel antibiotics to bacterial ribosomes in the macrolide-binding site.
authors.affiliation: Lomonosov Moscow State University, Faculty of Chemistry, Moscow, 119991, Russia. sumbtyan@belozersky.msu.ru.
authors.forename: A G
authors.initials: AG
authors.lastname: Tereshchenkov
authors.forename: A V
authors.initials: AV
authors.lastname: Shishkina
authors.forename: V V
authors.initials: VV
authors.lastname: Karpenko
authors.forename: V A
authors.initials: VA
authors.lastname: Chertkov
authors.forename: A L
authors.initials: AL
authors.lastname: Konevega
authors.forename: P S
authors.initials: PS
authors.lastname: Kasatsky
authors.forename: A A
authors.initials: AA
authors.lastname: Bogdanov
authors.forename: N V
authors.initials: NV
authors.lastname: Sumbatyan
mesh_terms: D004926:Escherichia coli / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry*; D012270:Ribosomes / Q000737:chemistry*; D015645:Tylosin / Q000031:analogs & derivatives* / Q000737:chemistry*
chemical_list: D005456:Fluorescent Dyes; D015645:Tylosin