title: Involvement of BDNF/TrkB signaling in the effect of diphenyl diselenide on motor function in a Parkinson's disease rat model.
abstract: Parkinson's disease is a progressive neurodegenerative disorder characterized by degeneration of nigrostriatal dopaminergic neurons. Diphenyl diselenide [(PhSe)2] is a compound with pharmacological proprieties, such as antidepressant and neuroprotective. Therefore, this study investigated whether (PhSe)2reverses motor impairment and neurochemical alterations in a model of Parkinson's disease induced by 6-hydroxydopamine (6-OHDA) in rats. For this, male Wistar rats received 20μg/3μl of 6-OHDA or vehicle into the right striatum. Three weeks later, animals were subjected to rotational behavioral test induced by D-amphetamine and randomly divided into four groups: Sham; (PhSe)2; 6-OHDA and 6-OHDA+(PhSe)2. The rats received (PhSe)2(1mg/kg/day; i.g.) or vehicle (canola oil) during 30 days. After treatment, behavioral tests were performed in order to evaluate the motor function and the ipsilateral striatal tissue was collected for immunoblotting assay. (PhSe)2treatment restored the normal motor behavior of 6-OHDA-infused rats in the cylinder, stepping and bridge tests, but not in the rotarod test. The 6-OHDA infusion and/or (PhSe)2treatment did not alter the muscle strength and spontaneous locomotion in the forelimb support and open-field tests, respectively. Additionally, striatal brain-derived neurotrophic factor (BDNF), proBDNF and tyrosine hydroxylase (TH) levels of 6-OHDA-lesioned rats were decreased, while the tropomyosin-related kinase B (TrkB) levels were increased. (PhSe)2treatment restored striatal proBDNF, TrkB and TH levels. Thus, (PhSe)2treatment reversed some motor impairment and TH levels in a 6-OHDA model of Parkinson's disease in rats, demonstrating a potential neurorestorative role. Additionally, the BDNF/TrkB signaling recovery can be involved in its neurorestorative effect.
authors.affiliation: Departamento de Bioquímica e Biologia Molecular, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria, Santa Maria, CEP 97105-900 RS, Brazil.
authors.forename: Tuane Bazanella
authors.initials: TB
authors.lastname: Sampaio
authors.affiliation: Universidade Federal do Pampa - Campus Uruguaiana, Uruguaiana, CEP 97500-970 RS, Brazil. Electronic address: sipinton@gmail.com.
authors.forename: Simone
authors.initials: S
authors.lastname: Pinton
authors.affiliation: Universidade Federal de Ciências da Saúde de Porto Alegre, Porto Alegre, CEP 90050-170 RS, Brazil.
authors.forename: Juliana Trevisan
authors.initials: JT
authors.lastname: da Rocha
authors.affiliation: Instituto de Ciências Exatas e da Terra - ICET, Departamento de Química, Universidade Federal de Mato Grosso, Cuiabá, CEP 78060-900 MT, Brazil.
authors.forename: Bibiana Mozzaquatro
authors.initials: BM
authors.lastname: Gai
authors.affiliation: Departamento de Bioquímica e Biologia Molecular, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria, Santa Maria, CEP 97105-900 RS, Brazil.
authors.forename: Cristina Wayne
authors.initials: CW
authors.lastname: Nogueira
mesh_terms: D000818:Animals; D001369:Axons / Q000187:drug effects / Q000473:pathology; D001555:Benzene Derivatives / Q000494:pharmacology* / Q000627:therapeutic use; D019208:Brain-Derived Neurotrophic Factor / Q000378:metabolism*; D004195:Disease Models, Animal; D059290:Dopaminergic Neurons / Q000187:drug effects / Q000473:pathology; D008297:Male; D009043:Motor Activity / Q000187:drug effects*; D016566:Organoselenium Compounds / Q000494:pharmacology* / Q000627:therapeutic use; D016627:Oxidopamine / Q000494:pharmacology; D010300:Parkinson Disease / Q000188:drug therapy / Q000209:etiology / Q000473:pathology* / Q000503:physiopathology*; D051381:Rats; D017208:Rats, Wistar; D020813:Receptor, trkB / Q000378:metabolism*; D015398:Signal Transduction / Q000187:drug effects*; D013378:Substantia Nigra / Q000187:drug effects / Q000473:pathology
chemical_list: D001555:Benzene Derivatives; D019208:Brain-Derived Neurotrophic Factor; D016566:Organoselenium Compounds; C061132:diphenyldiselenide; D016627:Oxidopamine; D020813:Receptor, trkB
keywords: 6-Hydroxydopamine; 6-Hydroxydopamine hydrochloride (PubChem CID: 160157); Brain-derived neurotrophic factor; D-Amphetamine (PubChem CID: 5826); Diphenyl diselenide (PubChem CID: 15460); Motor impairment; Selenium