title: A single-step method for RNA isolation from tropical crops in the field.
abstract: The RNAzol RT reagent was used to provide pure RNA from human cells. We develop a protocol using RNAzol RT reagent to extract pure RNA from plants tissues and demonstrate that this RNA extraction method works not only at room temperature but also at elevated temperatures and provides the simplest and most effective single-step method to extract pure and undegraded RNA directly from tropical plants in the field. RNA extraction directly in a complex field environment opens up the way for studying gene-environment interactions at transcriptome level to decipher the complex regulatory network involved in multiple-stress responses.
authors.affiliation: CIRAD, UMR IPME, 911 Avenue Agropolis, BP 64501, 34394, Montpellier, France.
authors.forename: J-C
authors.initials: JC
authors.lastname: Breitler
authors.affiliation: IRD, UMR IPME, 911 Avenue Agropolis, BP 64501, 34394, Montpellier, France.
authors.forename: C
authors.initials: C
authors.lastname: Campa
authors.affiliation: CIRAD, UMR IPME, 911 Avenue Agropolis, BP 64501, 34394, Montpellier, France.
authors.forename: F
authors.initials: F
authors.lastname: Georget
authors.affiliation: CIRAD, UMR IPME, 911 Avenue Agropolis, BP 64501, 34394, Montpellier, France.
authors.forename: B
authors.initials: B
authors.lastname: Bertrand
authors.affiliation: CIRAD, UMR IPME, 911 Avenue Agropolis, BP 64501, 34394, Montpellier, France.
authors.forename: H
authors.initials: H
authors.lastname: Etienne
mesh_terms: D040503:Coffea / Q000737:chemistry*; D005638:Fruit / Q000737:chemistry*; D006801:Humans; D059625:Liquid-Liquid Extraction / Q000191:economics / Q000379:methods*; D002365:Manihot / Q000737:chemistry; D012275:Oryza / Q000737:chemistry; D018515:Plant Leaves / Q000737:chemistry*; D018517:Plant Roots / Q000737:chemistry*; D018749:RNA, Plant / Q000302:isolation & purification*; D015203:Reproducibility of Results; D013696:Temperature; D003313:Zea mays / Q000737:chemistry
chemical_list: D018749:RNA, Plant