title: Purification of common light chain IgG-like bispecific antibodies using highly linear pH gradients.
abstract: Monovalent bispecific antibodies (BsAbs) are projected to have broad clinical applications due to their ability to bind two different targets simultaneously. Although they can be produced using recombinant technologies, the correct pairing of heavy and light chains is a significant manufacturing problem. Various approaches exploit mutations or linkers to favor the formation of the desired BsAb, but a format using a single common light chain has the advantage that no other modification to the antibody is required. This strategy reduces the number of formed molecules to three (the BsAb and the two parent mAbs), but the separation of the BsAb from the two monovalent parent molecules still poses a potentially difficult purification challenge. Current methods employ ion exchange chromatography and linear salt gradients, but are only successful if the difference in the observed isoelectric points (pIs) of two parent molecules is relatively large. Here, we describe the use of highly linear pH gradients for the facile purification of common light chain BsAbs. The method is effective at separating molecules with differences in pI as little as 0.10, and differing in their sequence by only a single charged amino acid. We also demonstrate that purification resins validated for manufacturing are compatible with this approach.
authors.affiliation: a Department of High-Throughput Expression , Adimab LLC , Lebanon , NH , USA.
authors.forename: Beth
authors.initials: B
authors.lastname: Sharkey
authors.affiliation: a Department of High-Throughput Expression , Adimab LLC , Lebanon , NH , USA.
authors.forename: Sarat
authors.initials: S
authors.lastname: Pudi
authors.affiliation: a Department of High-Throughput Expression , Adimab LLC , Lebanon , NH , USA.
authors.forename: Ian
authors.initials: I
authors.lastname: Wallace Moyer
authors.affiliation: a Department of High-Throughput Expression , Adimab LLC , Lebanon , NH , USA.
authors.forename: Lihui
authors.initials: L
authors.lastname: Zhong
authors.affiliation: b Department of Antibody Discovery , Adimab LLC , Lebanon , NH , USA.
authors.forename: Bianka
authors.initials: B
authors.lastname: Prinz
authors.affiliation: b Department of Antibody Discovery , Adimab LLC , Lebanon , NH , USA.
authors.forename: Hemanta
authors.initials: H
authors.lastname: Baruah
authors.affiliation: c Department of Protein Analytics , Adimab LLC , Lebanon , NH , USA.
authors.forename: Heather
authors.initials: H
authors.lastname: Lynaugh
authors.affiliation: a Department of High-Throughput Expression , Adimab LLC , Lebanon , NH , USA.
authors.forename: Sampath
authors.initials: S
authors.lastname: Kumar
authors.affiliation: a Department of High-Throughput Expression , Adimab LLC , Lebanon , NH , USA.|b Department of Antibody Discovery , Adimab LLC , Lebanon , NH , USA.|c Department of Protein Analytics , Adimab LLC , Lebanon , NH , USA.
authors.forename: K Dane
authors.initials: KD
authors.lastname: Wittrup
authors.affiliation: a Department of High-Throughput Expression , Adimab LLC , Lebanon , NH , USA.
authors.forename: Juergen H
authors.initials: JH
authors.lastname: Nett
mesh_terms: D018033:Antibodies, Bispecific / Q000302:isolation & purification*; D002852:Chromatography, Ion Exchange / Q000379:methods*; D006801:Humans; D007074:Immunoglobulin G / Q000302:isolation & purification*; D015202:Protein Engineering / Q000379:methods; D018892:Proton-Motive Force
chemical_list: D018033:Antibodies, Bispecific; D007074:Immunoglobulin G
keywords: Bispecific antibody; common light chain; ion exchange chromatography; isoelectric point; linear pH gradient; purification