title: Targeted proteomics of cannabinoid receptor CB1 and the CB1b isoform.
abstract: Cannabinoid receptors (CBR), including CB1 and CB2 have been therapeutic targets for a number of conditions. Recently, splice variants of the CB1R have been identified in humans. The isoforms differ in their N-terminus sequence and pharmacological activity relative to the CB1R, as a result, the differentiation between the CB1 receptor and its isoform is required. As a result, a selected reaction monitoring mass spectrometry (SRM-MS) method was developed for the quantitation of CB1 and the CB1b isoform in CHO cells transduced with CB1 and CB1b. The SRM-MS protocol was assessed with isotopically labeled peptide standards and had high reproducibility of intra-day assay (CVs from 1.9 to 4.3% for CB1 and 0.5 to 5.9% for CB1b) and inter-day assay (CVs from 1.2 to 5.2% for CB1 and 1.2 to 6.1% for CB1b).
authors.affiliation: Biomedical Research Center, National Institute on Aging, National Institutes of Health, 251 Bayview Boulevard, Baltimore, MD 21224, United States.
authors.forename: Soumita
authors.initials: S
authors.lastname: Ghosh
authors.affiliation: Biomedical Research Center, National Institute on Aging, National Institutes of Health, 251 Bayview Boulevard, Baltimore, MD 21224, United States.
authors.forename: Isabel
authors.initials: I
authors.lastname: González-Mariscal
authors.affiliation: Biomedical Research Center, National Institute on Aging, National Institutes of Health, 251 Bayview Boulevard, Baltimore, MD 21224, United States.
authors.forename: Josephine M
authors.initials: JM
authors.lastname: Egan
authors.affiliation: Biomedical Research Center, National Institute on Aging, National Institutes of Health, 251 Bayview Boulevard, Baltimore, MD 21224, United States. Electronic address: moaddelru@mail.nih.gov.
authors.forename: Ruin
authors.initials: R
authors.lastname: Moaddel
mesh_terms: D000818:Animals; D016466:CHO Cells; D006224:Cricetinae; D003412:Cricetulus; D006801:Humans; D020033:Protein Isoforms; D040901:Proteomics; D043884:Receptor, Cannabinoid, CB1 / Q000378:metabolism*; D015203:Reproducibility of Results
chemical_list: D020033:Protein Isoforms; D043884:Receptor, Cannabinoid, CB1
keywords: CB1; CB1b; Cannabinoid receptor isoforms; SRM assay