title: Redox-Mediated Disassembly to Build Activatable Trimodal Probe for Molecular Imaging of Biothiols.
abstract: Activatable multimodal probes that show enhancement of multiplex imaging signals upon interaction with their specific molecular target have become powerful tools for rapid and precise imaging of biological processes. Herein, we report a stimuli-responsive disassembly approach to construct a redox-activatable fluorescence/19F-MRS/1H-MRI triple-functional probe 1. The small molecule probe 1 itself has a high propensity to self-assemble into nanoparticles with quenched fluorescence, attenuated19F-MRS signal, and high1H-MRI contrast. Biothiols that are abundant in reducing biological environment were able to cleave the disulfide bond in probe 1 to induce disassembly of the nanoparticles and lead to fluorescence activation (∼70-fold),19F-MRS signal amplification (∼30-fold) and significant r1relaxivity reduction (∼68% at 0.5 T). Molecular imaging of reducing environment in live cells and in vivo was realized using probe 1. This approach could facilitate the development of other stimuli-responsive trimodal probes for molecular imaging.
authors.affiliation: State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University , Nanjing, 210093, China.
authors.forename: Mengmeng
authors.initials: M
authors.lastname: Zheng
authors.affiliation: State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University , Nanjing, 210093, China.
authors.forename: Yuqi
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Radiology, Drum Tower Hospital, School of Medicine, Nanjing University , Nanjing, 210008, China.
authors.forename: Hua
authors.initials: H
authors.lastname: Shi
authors.affiliation: State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University , Nanjing, 210093, China.
authors.forename: Yuxuan
authors.initials: Y
authors.lastname: Hu
authors.affiliation: State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University , Nanjing, 210093, China.
authors.forename: Liandong
authors.initials: L
authors.lastname: Feng
authors.affiliation: State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University , Nanjing, 210093, China.
authors.forename: Zhiliang
authors.initials: Z
authors.lastname: Luo
authors.affiliation: State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University , Nanjing, 210093, China.
authors.forename: Mi
authors.initials: M
authors.lastname: Zhou
authors.affiliation: Department of Radiology, Drum Tower Hospital, School of Medicine, Nanjing University , Nanjing, 210008, China.
authors.forename: Jian
authors.initials: J
authors.lastname: He
authors.affiliation: Department of Radiology, Drum Tower Hospital, School of Medicine, Nanjing University , Nanjing, 210008, China.
authors.forename: Zhenyang
authors.initials: Z
authors.lastname: Zhou
authors.affiliation: State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University , Nanjing, 210093, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University , Nanjing, 210093, China.
authors.forename: Deju
authors.initials: D
authors.lastname: Ye
mesh_terms: D066235:Fluorine-19 Magnetic Resonance Imaging; D008279:Magnetic Resonance Imaging; D057054:Molecular Imaging; D053758:Nanoparticles; D010084:Oxidation-Reduction
keywords: activatable probe; disassembly; imaging; multimodality; redox