title: Diversity of Cultivable Midgut Microbiota at Different Stages of the Asian Tiger Mosquito, Aedes albopictus from Tezpur, India.
abstract: Aedes aegypti and Ae. albopictus are among the most important vectors of arboviral diseases, worldwide. Recent studies indicate that diverse midgut microbiota of mosquitoes significantly affect development, digestion, metabolism, and immunity of their hosts. Midgut microbiota has also been suggested to modulate the competency of mosquitoes to transmit arboviruses, malaria parasites etc. Interestingly, the midgut microbial flora is dynamic and the diversity changes with the development of vectors, in addition to other factors such as species, sex, life-stage, feeding behavior and geographical origin. The aim of the present study was to investigate the midgut bacterial diversity among larva, adult male, sugar fed female and blood fed female Ae. albopictus collected from Tezpur, Northeastern India. Based on colony morphological characteristics, we selected 113 cultivable bacterial isolates for 16S rRNA gene sequence based molecular identification. Of the 113 isolates, we could identify 35 bacterial species belonging to 18 distinct genera under four major phyla, namely Proteobacteria, Firmicutes, Actinobacteria and Bacteroidetes. Phyla Proteobacteria and Firmicutes accounted for majority (80%) of the species, while phylum Actinobacteria constituted 17% of the species. Bacteroidetes was the least represented phylum, characterized by a single species- Chryseobacterium rhizoplanae, isolated from blood fed individuals. Dissection of midgut microbiota diversity at different developmental stages of Ae. albopictus will be helpful in better understanding mosquito-borne diseases, and for designing effective strategies to manage mosquito-borne diseases.
authors.affiliation: Biotechnology Division, Defence Research Laboratory, DRDO, Tezpur, India.
authors.forename: Kamlesh K
authors.identifier: http://orcid.org/0000-0003-3871-9937
authors.initials: KK
authors.lastname: Yadav
authors.affiliation: Biotechnology Division, Defence Research Laboratory, DRDO, Tezpur, India.
authors.forename: Sibnarayan
authors.initials: S
authors.lastname: Datta
authors.affiliation: Biotechnology Division, Defence Research Laboratory, DRDO, Tezpur, India.
authors.forename: Ashok
authors.initials: A
authors.lastname: Naglot
authors.affiliation: Biotechnology Division, Defence Research Laboratory, DRDO, Tezpur, India.
authors.forename: Ajitabh
authors.initials: A
authors.lastname: Bora
authors.affiliation: Biotechnology Division, Defence Research Laboratory, DRDO, Tezpur, India.
authors.forename: Vanlal
authors.initials: V
authors.lastname: Hmuaka
authors.affiliation: School of Studies in Biotechnology, Jiwaji University, Gwalior, India.
authors.forename: Sameer
authors.initials: S
authors.lastname: Bhagyawant
authors.affiliation: Biotechnology Division, Defence Research Laboratory, DRDO, Tezpur, India.
authors.forename: Hemanta K
authors.initials: HK
authors.lastname: Gogoi
authors.affiliation: Biotechnology Division, Defence Research Laboratory, DRDO, Tezpur, India.
authors.forename: Vijay
authors.initials: V
authors.lastname: Veer
authors.affiliation: Biotechnology Division, Defence Research Laboratory, DRDO, Tezpur, India.
authors.forename: P Srinivas
authors.initials: PS
authors.lastname: Raju
mesh_terms: D000330:Aedes; D000818:Animals; D001419:Bacteria / Q000145:classification / Q000235:genetics; D044822:Biodiversity; D005260:Female; D000069196:Gastrointestinal Microbiome; D007194:India; D008018:Life Cycle Stages; D008297:Male; D054892:Metagenome; D056186:Metagenomics / Q000379:methods; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics
chemical_list: D012336:RNA, Ribosomal, 16S