title: Amelioration of Experimental Autoimmune Encephalomyelitis by Isogarcinol Extracted from Garcinia mangostana L. Mangosteen.
abstract: Isogarcinol is a new natural immunosuppressant that was extracted from Garcinia mangostana L. in our laboratory. Knowledge of its effects on treatable diseases and its mechanism of action is still very limited. In this study, we explored the therapeutic effect of isogarcinol in experimental autoimmune encephalomyelitis (EAE), a murine model of multiple sclerosis (MS). Treatment with oral 100 mg/kg isogarcinol markedly ameliorated clinical scores, alleviated inflammation and demyelination of the spinal cord, and reduced intracranial lesions in EAE mice. The percentages of Th cells and macrophages were also strongly reduced. Isogarcinol appeared to act by inhibiting T helper (Th) 1 and Th17 cell differentiation via the janus kinase/signal transducers and activators of transcription pathway and by impairing macrophage function. Our data suggest that isogarcinol has the potential to be an effective therapeutic agent of low toxicity for treating MS and other autoimmune diseases.
authors.affiliation: Gene Engineering and Biotechnology Beijing Key Laboratory, Department of Biochemistry and Molecular Biology, Beijing Normal University , Beijing 100875, People's Republic of China.
authors.forename: Mengqi
authors.initials: M
authors.lastname: Wang
authors.affiliation: Gene Engineering and Biotechnology Beijing Key Laboratory, Department of Biochemistry and Molecular Biology, Beijing Normal University , Beijing 100875, People's Republic of China.
authors.forename: Yufei
authors.initials: Y
authors.lastname: Xie
authors.affiliation: Gene Engineering and Biotechnology Beijing Key Laboratory, Department of Biochemistry and Molecular Biology, Beijing Normal University , Beijing 100875, People's Republic of China.|National Vaccine and Serum Institute, Beijing 100024, People's Republic of China.
authors.forename: Youxiu
authors.initials: Y
authors.lastname: Zhong
authors.affiliation: Gene Engineering and Biotechnology Beijing Key Laboratory, Department of Biochemistry and Molecular Biology, Beijing Normal University , Beijing 100875, People's Republic of China.|Key Laboratory of Protection and Development Utilization of Tropical Crop Germplasm Resources, Ministry of Education, College of Landscape and Horticulture, Hainan University , Haikou 570228, People's Republic of China.
authors.forename: Juren
authors.initials: J
authors.lastname: Cen
authors.affiliation: Gene Engineering and Biotechnology Beijing Key Laboratory, Department of Biochemistry and Molecular Biology, Beijing Normal University , Beijing 100875, People's Republic of China.
authors.forename: Lei
authors.initials: L
authors.lastname: Wang
authors.affiliation: Gene Engineering and Biotechnology Beijing Key Laboratory, Department of Biochemistry and Molecular Biology, Beijing Normal University , Beijing 100875, People's Republic of China.
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Gene Engineering and Biotechnology Beijing Key Laboratory, Department of Biochemistry and Molecular Biology, Beijing Normal University , Beijing 100875, People's Republic of China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhu
authors.affiliation: Gene Engineering and Biotechnology Beijing Key Laboratory, Department of Biochemistry and Molecular Biology, Beijing Normal University , Beijing 100875, People's Republic of China.
authors.forename: Li
authors.initials: L
authors.lastname: Tong
authors.affiliation: Gene Engineering and Biotechnology Beijing Key Laboratory, Department of Biochemistry and Molecular Biology, Beijing Normal University , Beijing 100875, People's Republic of China.
authors.forename: Qun
authors.initials: Q
authors.lastname: Wei
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology; D002454:Cell Differentiation / Q000187:drug effects; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000188:drug therapy*; D005260:Female; D029762:Garcinia mangostana / Q000737:chemistry*; D007166:Immunosuppressive Agents / Q000494:pharmacology*; D007249:Inflammation / Q000188:drug therapy; D053612:Janus Kinases / Q000378:metabolism; D008264:Macrophages / Q000187:drug effects; D051379:Mice; D008810:Mice, Inbred C57BL; D010936:Plant Extracts / Q000494:pharmacology*; D050794:STAT1 Transcription Factor / Q000037:antagonists & inhibitors / Q000378:metabolism; D050796:STAT3 Transcription Factor / Q000037:antagonists & inhibitors / Q000378:metabolism; D015398:Signal Transduction; D013116:Spinal Cord / Q000187:drug effects / Q000378:metabolism; D013729:Terpenes / Q000494:pharmacology*; D058504:Th17 Cells
chemical_list: D000893:Anti-Inflammatory Agents; D007166:Immunosuppressive Agents; D010936:Plant Extracts; D050794:STAT1 Transcription Factor; D050796:STAT3 Transcription Factor; C494080:Stat1 protein, mouse; C494087:Stat3 protein, mouse; D013729:Terpenes; C000617954:isogarcinol; D053612:Janus Kinases; C054597:garcinol
keywords: Garcinia mangostana L.; Jak/STAT pathway; cytokines; experimental autoimmune encephalomyelitis; isogarcinol; multiple sclerosis