title: Immunoreactivities of NF-κB, IL-1β and IL-1R in the skin of Chinese brown frog (Rana dybowskii).
abstract: The cytokine interleukin-1 beta (IL-1β) is an evolutionarily conserved molecule that was originally identified in the immune system. Nuclear factor κB (NF-κB) plays a critical role in the activation of immune cells by upregulating the expression of many cytokines. In this study, we investigated the localization and expression level of IL-1β, its functional membrane receptor type I (IL-1R1) and NF-κB in the skin of Rana dybowskii during the breeding period and pre-hibernation. Histologically, the skin of Rana dybowskii consists of epidermis and dermis, and four kinds of cells were identified in the epidermis during the breeding period and pre-hibernation, while the dermis was composed of homogenous gel, mucous glands and granular glands. IL-1β, IL-1R1 and NF-κB were immunolocalized in the epithelial and glandular cells in both periods. Western blotting showed that there was no significant difference in the expression of IL-1β between the breeding period and pre-hibernation, whereas IL-1R1 and NF-κB were significantly higher in the pre-hibernation compared to the breeding period. These results suggested that IL-1β and NF-κB may collectively play important roles in the skin immune system of Rana dybowskii during the breeding period and pre-hibernation.
authors.affiliation: College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, PR China.
authors.forename: Liqin
authors.initials: L
authors.lastname: Xi
authors.affiliation: College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, PR China.
authors.forename: Ruiqi
authors.initials: R
authors.lastname: Hu
authors.affiliation: College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, PR China.
authors.forename: Ting
authors.initials: T
authors.lastname: Guo
authors.affiliation: College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, PR China.
authors.forename: Yuhan
authors.initials: Y
authors.lastname: Wang
authors.affiliation: College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, PR China.
authors.forename: Xia
authors.initials: X
authors.lastname: Sheng
authors.affiliation: College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, PR China.
authors.forename: Yingying
authors.initials: Y
authors.lastname: Han
authors.affiliation: College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, PR China.
authors.forename: Zhengrong
authors.initials: Z
authors.lastname: Yuan
authors.affiliation: College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, PR China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Weng
authors.affiliation: College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, PR China. Electronic address: xumeiyu@bjfu.edu.cn.
authors.forename: Meiyu
authors.initials: M
authors.lastname: Xu
mesh_terms: D000818:Animals; D020405:Dermis / Q000276:immunology* / Q000648:ultrastructure; D004817:Epidermis / Q000276:immunology* / Q000648:ultrastructure; D004847:Epithelial Cells / Q000276:immunology / Q000648:ultrastructure; D005260:Female; D005786:Gene Expression Regulation; D006605:Hibernation / Q000235:genetics / Q000276:immunology; D007113:Immunity, Innate; D007150:Immunohistochemistry; D053583:Interleukin-1beta / Q000235:genetics / Q000276:immunology*; D008297:Male; D016328:NF-kappa B / Q000235:genetics / Q000276:immunology*; D011898:Ranidae; D053573:Receptors, Interleukin-1 Type I / Q000235:genetics / Q000276:immunology*; D012098:Reproduction / Q000235:genetics / Q000276:immunology
chemical_list: D053583:Interleukin-1beta; D016328:NF-kappa B; D053573:Receptors, Interleukin-1 Type I
keywords: IL-1R1; IL-1β; NF-κB; Rana dybowskii; Skin