title: Bioactive Terpenoids from Salvia plebeia: Structures, NO Inhibitory Activities, and Interactions with iNOS.
abstract: A phytochemical investigation to obtain new NO inhibitors resulted in the identification of six new (1-6) and four known (7-10) terpenoids from Salvia plebeia. Compounds 1 and 2 are new diterpenoids, 3-5 are new meroditerpenoids, 6-9 are sesquiterpenoids, and 10 is a known meroditerpenoid. The structures of these isolates were determined by routine NMR experiments and X-ray diffraction, as well as the electronic circular dichroism spectra. Compounds 1-4 are diterpenoids carrying an oxygen bridge, and 6 is a rare copane-type sesquiterpenoid with a bridged tricyclic framework. The isolates inhibited NO generation induced by lipopolysaccharide in BV-2 cells. The possible mechanism of NO inhibition of some bioactive compounds was also investigated using molecular docking, which revealed interactions of bioactive compounds with the iNOS protein.
authors.forename: Jing
authors.initials: J
authors.lastname: Xu
authors.forename: Meicheng
authors.initials: M
authors.lastname: Wang
authors.forename: Xiaocong
authors.initials: X
authors.lastname: Sun
authors.forename: Quanhui
authors.initials: Q
authors.lastname: Ren
authors.forename: Xiangrong
authors.initials: X
authors.lastname: Cao
authors.forename: Shen
authors.initials: S
authors.lastname: Li
authors.forename: Guochen
authors.initials: G
authors.lastname: Su
authors.affiliation: College of Chemistry and Environmental Sciences, Laboratory of Xinjiang Native Medicinal and Edible Plant Resources Chemistry, Kashgar University , Kashgar 844000, People's Republic of China.
authors.forename: Muhetaer
authors.initials: M
authors.lastname: Tuerhong
authors.affiliation: Department of Biosystems and Biotechnology, College of Life Sciences and Biotechnology, Korea University , Seoul 02841, Republic of Korea.
authors.forename: Dongho
authors.initials: D
authors.lastname: Lee
authors.affiliation: Department of Medical Biochemistry, School of Pharmaceutical Sciences, University of Shizuoka , Shizuoka, Japan.
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Ohizumi
authors.forename: Mark
authors.initials: M
authors.lastname: Bartlam
authors.forename: Yuanqiang
authors.initials: Y
authors.lastname: Guo
mesh_terms: D018360:Crystallography, X-Ray; D004224:Diterpenes / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D008070:Lipopolysaccharides / Q000494:pharmacology; D008264:Macrophages / Q000187:drug effects; D008968:Molecular Conformation; D062105:Molecular Docking Simulation; D015394:Molecular Structure; D009569:Nitric Oxide / Q000037:antagonists & inhibitors* / Q000096:biosynthesis; D052247:Nitric Oxide Synthase Type II / Q000378:metabolism*; D019906:Nuclear Magnetic Resonance, Biomolecular; D027544:Salvia / Q000737:chemistry*; D012717:Sesquiterpenes / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology
chemical_list: D004224:Diterpenes; D004365:Drugs, Chinese Herbal; D008070:Lipopolysaccharides; D012717:Sesquiterpenes; D009569:Nitric Oxide; C496319:NOS2 protein, human; D052247:Nitric Oxide Synthase Type II