title: Overexpression ofPanax ginsengsesquiterpene synthase gene confers tolerance againstPseudomonas syringaepv.tomatoinArabidopsis thaliana.
abstract: Sesquiterpenes are an abundant group belonging to the terpenoid family, with a C15 structure comprise of three isoprene units. Many sesquiterpenes are volatile compounds and it act as chemical messenger in plant signalling, particularly in the defense mechanism against biotic and abiotic stresses.Panax ginsengMeyer is important medicinal herbs with various reported pharmacological efficacies in which its triterpenoid saponins, called ginsenosides, were mostly studied. However, there have been few studies on volatile sesquiterpenes compounds regulation onP. ginseng. As slow-growing perennial plant,P. ginsengreceived many kind of stresses during its cultivation. The pathogen attack is one of the most devastated perturbation for ginseng yield. Thus, we aimed to analyzeP. ginseng STSgene (PgSTS) expressions in ginseng organs as well as mono-, sesquiterpenes contents from ginseng seedlings treated with elicitors. qRT-PCR and GC-MS analysis showed that two elicitors- salicylic acid (SA) and methyl jasmonate (MeJA) triggeredPgSTSexpression at different time points and significantly induced mono-, sesquiterpene yield. Overexpression ofPgSTSinArabidopsisalso induced high terpene content and conferred tolerance againstPseudomonas syringaepv.tomatoinfection. These results suggested thatPgSTStranscripts are involved in terpenoid biosynthesis in response to environmental stress mediated by MeJA and SA elicitors; thus, generate tolerance against pathogen attack.
authors.affiliation: Department of Oriental Medicinal Biotechnology, College of Life Science, Kyung Hee University, Yongin, 449-701 Korea.
authors.forename: Sung-Joo
authors.initials: SJ
authors.lastname: Yoon
authors.affiliation: Graduate School of Biotechnology, College of Life Science, Kyung Hee University, Yongin, 446- 701 Korea.
authors.forename: Johan
authors.initials: J
authors.lastname: Sukweenadhi
authors.affiliation: Department of Oriental Medicinal Biotechnology, College of Life Science, Kyung Hee University, Yongin, 449-701 Korea.
authors.forename: Altanzul
authors.initials: A
authors.lastname: Khorolragchaa
authors.affiliation: Graduate School of Biotechnology, College of Life Science, Kyung Hee University, Yongin, 446- 701 Korea.
authors.forename: Ramya
authors.initials: R
authors.lastname: Mathiyalagan
authors.affiliation: Department of Oriental Medicinal Biotechnology, College of Life Science, Kyung Hee University, Yongin, 449-701 Korea.
authors.forename: Sathiyamoorthy
authors.initials: S
authors.lastname: Subramaniyam
authors.affiliation: Department of Oriental Medicinal Biotechnology, College of Life Science, Kyung Hee University, Yongin, 449-701 Korea.
authors.forename: Yeon-Ju
authors.initials: YJ
authors.lastname: Kim
authors.affiliation: Woongjin Foods Co., Ltd., JEI-PLATZ, 186, Gasan Digital 1-ro, Room 201, Gemcheon-gu, Seoul, 153-792 Korea.
authors.forename: Ho-Bin
authors.initials: HB
authors.lastname: Kim
authors.affiliation: Woongjin Foods Co., Ltd., JEI-PLATZ, 186, Gasan Digital 1-ro, Room 201, Gemcheon-gu, Seoul, 153-792 Korea.
authors.forename: Mi-Jung
authors.initials: MJ
authors.lastname: Kim
authors.affiliation: Department of Oriental Medicinal Biotechnology, College of Life Science, Kyung Hee University, Yongin, 449-701 Korea.
authors.forename: Yu-Jin
authors.identifier: 0000-0001-6615-7447
authors.initials: YJ
authors.lastname: Kim
authors.affiliation: Department of Oriental Medicinal Biotechnology, College of Life Science, Kyung Hee University, Yongin, 449-701 Korea.|Graduate School of Biotechnology, College of Life Science, Kyung Hee University, Yongin, 446- 701 Korea.
authors.forename: Deok-Chun
authors.initials: DC
authors.lastname: Yang
keywords: Gene expression; Ginseng; Methyl jasmonate; Salicylic acid; Sesquiterpene synthase; Terpene content