title: PCR ITS-RFLP for Penicillium Species and Other Genera.
abstract: Among numerous molecular methodologies developed for highly specific identification of filamentous fungi isolates, here we describe restriction digestion analysis of the ITS products as an easy method to identify isolates of filamentous fungi. This technique is a rapid and reliable method appropriate for routine identification of filamentous fungi. This can be used to screen large numbers of isolates from various environments in a short time. The use of different endonucleases allowed generating individual restriction profiles. The individual profiles obtained were combined into composite restriction patterns characteristic of a species. Eleven different genera can be differentiated and among them 41 different species.
authors.affiliation: Institut Universitaire de la Vigne et du Vin « Jules Guyot », Université de Bourgogne, BP 27877, 2 Rue Claude Ladrey, Dijon Cedex, 21078, France. sandrine.rousseaux@u-bourgogne.fr.
authors.forename: Sandrine
authors.initials: S
authors.lastname: Rousseaux
authors.affiliation: Institut Universitaire de la Vigne et du Vin « Jules Guyot », Université de Bourgogne, BP 27877, 2 Rue Claude Ladrey, Dijon Cedex, 21078, France.
authors.forename: Michèle
authors.initials: M
authors.lastname: Guilloux-Bénatier
mesh_terms: D021903:DNA, Ribosomal Spacer / Q000235:genetics*; D005658:Fungi / Q000145:classification* / Q000235:genetics*; D010407:Penicillium / Q000145:classification* / Q000235:genetics*; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length
chemical_list: D021903:DNA, Ribosomal Spacer
keywords: Composite profiles; Filamentous fungi; Identification; PCR ITS-RFLP