title: Comparative Evaluation of Four Phenotypic Tests for Detection of Carbapenemase-Producing Gram-Negative Bacteria.
abstract: Four screening assays aimed for rapid detection of carbapenemase production from Gram-negative bacterial isolates, i.e., the Neo-Rapid Carb kit (Rosco Diagnostica A/S), the Rapidec Carba NP test (bioMérieux SA), the β Carba test (Bio-Rad Laboratories N.V.), and a homemade electrochemical assay (BYG Carba test) were evaluated against a panel comprising 328 clinical isolates (Enterobacteriaceae [n = 198] and nonfermentative Gram-negative bacilli [n = 130]) with previously characterized resistance mechanisms to carbapenems. Among Enterobacteriaceae isolates, the BYG Carba test and the β Carba test showed excellent sensitivities (respectively, 100% and 97.3%) and specificities (respectively, 98.9% and 97.7%). The two other assays yielded poorer performances with sensitivity and specificity of 91.9% and 83.9% for the Rapidec Carba NP test and of 89.2% and 89.7% for the Neo-Rapid Carb kit, respectively. Among Pseudomonas spp., sensitivities and specificities ranged, respectively, from 87.3% to 92.7% and from 88.2% to 94.1%. Finally, all tests performed poorly against Acinetobacter spp., with sensitivities and specificities, respectively, ranging from 27.3% to 75.8% and from 75 to 100%. Among commercially available assays, the β Carba test appeared to be the most convenient for routine use and showed the best overall performances, especially against OXA-48-like producers. The excellent performance of the BYG Carba test against Enterobacteriaceae was confirmed (100% sensitivity and 98.9% specificity).
authors.affiliation: National Reference Laboratory for Antibiotic-Resistant Gram-Negative Bacilli, CHU UCL Namur, Yvoir, Belgium.
authors.forename: Audrey
authors.initials: A
authors.lastname: Noël
authors.affiliation: National Reference Laboratory for Antibiotic-Resistant Gram-Negative Bacilli, CHU UCL Namur, Yvoir, Belgium te-din.huang@uclouvain.be.
authors.forename: Te-Din
authors.initials: TD
authors.lastname: Huang
authors.affiliation: National Reference Laboratory for Antibiotic-Resistant Gram-Negative Bacilli, CHU UCL Namur, Yvoir, Belgium.
authors.forename: Catherine
authors.initials: C
authors.lastname: Berhin
authors.affiliation: National Reference Laboratory for Antibiotic-Resistant Gram-Negative Bacilli, CHU UCL Namur, Yvoir, Belgium.
authors.forename: Martin
authors.initials: M
authors.lastname: Hoebeke
authors.affiliation: National Reference Laboratory for Antibiotic-Resistant Gram-Negative Bacilli, CHU UCL Namur, Yvoir, Belgium.
authors.forename: Warda
authors.initials: W
authors.lastname: Bouchahrouf
authors.affiliation: National Reference Laboratory for Antibiotic-Resistant Gram-Negative Bacilli, CHU UCL Namur, Yvoir, Belgium.
authors.forename: Sami
authors.initials: S
authors.lastname: Yunus
authors.affiliation: National Reference Laboratory for Antibiotic-Resistant Gram-Negative Bacilli, CHU UCL Namur, Yvoir, Belgium.
authors.forename: Pierre
authors.initials: P
authors.lastname: Bogaerts
authors.affiliation: National Reference Laboratory for Antibiotic-Resistant Gram-Negative Bacilli, CHU UCL Namur, Yvoir, Belgium.
authors.forename: Youri
authors.initials: Y
authors.lastname: Glupczynski
mesh_terms: D000150:Acinetobacter / Q000201:enzymology* / Q000302:isolation & purification; D001426:Bacterial Proteins / Q000032:analysis*; D001431:Bacteriological Techniques / Q000379:methods*; D004755:Enterobacteriaceae / Q000201:enzymology* / Q000302:isolation & purification; D016905:Gram-Negative Bacterial Infections / Q000175:diagnosis* / Q000382:microbiology; D006801:Humans; D008403:Mass Screening / Q000379:methods*; D011446:Prospective Studies; D011549:Pseudomonas / Q000201:enzymology* / Q000302:isolation & purification; D012680:Sensitivity and Specificity; D001618:beta-Lactamases / Q000032:analysis*
chemical_list: D001426:Bacterial Proteins; D001618:beta-Lactamases; C063912:carbapenemase
keywords: Acinetobacter; CPE; Pseudomonas; antibiotic resistance; carbapenem hydrolysis; carbapenemase screening