title: Identification of specific biomarkers for gastric adenocarcinoma by ITRAQ proteomic approach.
abstract: The aim of this study was to identify biomarkers for gastric cancer (GC) by iTRAQ. Using proteins extracted from a panel of 4 pairs of gastric adenocarcinoma samples (stage III-IV, Her-2 negative), we identified 10 up regulated and 9 down regulated proteins in all four pairs of GC samples compared to adjacent normal gastric tissue. The up regulated proteins are mainly involved in cell motility, while the down regulated proteins are mitochondrial enzymes involved in energy metabolism. The expression of three up regulated proteins (ANXA1, NNMT, fibulin-5) and one of the down regulated proteins (UQCRC1) was validated by Western Blot in 97 GC samples. ANXA1 was up regulated in 61.36% of stage I/II GC samples compared to matched adjacent normal gastric tissue, and its expression increased further in stage III/IV samples. Knockdown of ANXA1 by siRNA significantly inhibited GC cell migration and invasion, whereas over expression of ANXA1 promoted migration and invasion. We found decreased expression of UQCRC1 in all stages of GC samples. Our data suggest that increased cell motility and decreased mitochondrial energy metabolism are important hallmarks during the development of GC.
authors.affiliation: Cyrus Tang Hematology Center, Collaborative Innovation Center of Hematology, Jiangsu Institute of Hematology, First Affiliated Hospital, Soochow University, Suzhou, China.
authors.forename: Xiaoxiao
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of General Surgery, the First Affiliated Hospital of Soochow University, Suzhou, China.
authors.forename: Qiaoming
authors.initials: Q
authors.lastname: Zhi
authors.affiliation: Suzhou Vocational Health College, Suzhou Key Laboratory of Biotechnology for Laboratory Medicine, Suzhou 215009, Jiangsu Province, China.
authors.forename: Songbai
authors.initials: S
authors.lastname: Liu
authors.affiliation: Department of Hematology, the First Affiliated Hospital of Soochow University, Jiangsu Institute of Hematology, the First Affiliated Hospital of Soochow University; Collaborative Innovation Center of Hematology, Soochow University, Suzhou, China.
authors.forename: Sheng-Li
authors.initials: SL
authors.lastname: Xue
authors.affiliation: Cyrus Tang Hematology Center, Collaborative Innovation Center of Hematology, Jiangsu Institute of Hematology, First Affiliated Hospital, Soochow University, Suzhou, China.
authors.forename: Congcong
authors.initials: C
authors.lastname: Shen
authors.affiliation: Department of Cardiovascular Surgery &Institute of Cardiovascular Science, First Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215123, P. R. China.
authors.forename: Yangxin
authors.initials: Y
authors.lastname: Li
authors.affiliation: Cyrus Tang Hematology Center, Collaborative Innovation Center of Hematology, Jiangsu Institute of Hematology, First Affiliated Hospital, Soochow University, Suzhou, China.
authors.forename: Chaofan
authors.initials: C
authors.lastname: Wu
authors.affiliation: Department of Biostatistics, School of Public Health, Medical College of Soochow University, China.
authors.forename: Zaixiang
authors.initials: Z
authors.lastname: Tang
authors.affiliation: Department of Gastroenterology, the First Affiliated Hospital of Soochow University, China.
authors.forename: Weichang
authors.initials: W
authors.lastname: Chen
authors.affiliation: Cyrus Tang Hematology Center, Collaborative Innovation Center of Hematology, Jiangsu Institute of Hematology, First Affiliated Hospital, Soochow University, Suzhou, China.
authors.forename: Jenny Lee
authors.initials: JL
authors.lastname: Song
authors.affiliation: Cyrus Tang Hematology Center, Collaborative Innovation Center of Hematology, Jiangsu Institute of Hematology, First Affiliated Hospital, Soochow University, Suzhou, China.
authors.forename: Meiyu
authors.initials: M
authors.lastname: Bao
authors.affiliation: Cyrus Tang Hematology Center, Collaborative Innovation Center of Hematology, Jiangsu Institute of Hematology, First Affiliated Hospital, Soochow University, Suzhou, China.
authors.forename: Yao-Hua
authors.initials: YH
authors.lastname: Song
authors.affiliation: Department of General Surgery, the First Affiliated Hospital of Soochow University, Suzhou, China.
authors.forename: Jin
authors.initials: J
authors.lastname: Zhou
mesh_terms: D000230:Adenocarcinoma / Q000737:chemistry*; D000368:Aged; D014408:Biomarkers, Tumor / Q000032:analysis*; D015153:Blotting, Western; D002465:Cell Movement / Q000235:genetics; D004734:Energy Metabolism / Q000235:genetics; D005260:Female; D019085:Fluorescent Antibody Technique, Direct; D015972:Gene Expression Regulation, Neoplastic; D063990:Gene Ontology; D006801:Humans; D008297:Male; D008875:Middle Aged; D009361:Neoplasm Invasiveness; D009363:Neoplasm Proteins / Q000032:analysis*; D040901:Proteomics / Q000379:methods*; D013274:Stomach Neoplasms / Q000737:chemistry*; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D014408:Biomarkers, Tumor; D009363:Neoplasm Proteins