title: Transcriptional Response of Silkworm (Bombyx mori) Eggs to O₂ or HCl Treatment.
abstract: Diapause is a common biological phenomenon that occurs in many organisms, including fish, insects, and nematodes. In the silkworm (Bombyx mori), diapause generally occurs in the egg stage. Treatment with O₂, HCl, or other compounds can prevent egg diapause. Here, we characterized the transcriptomic responses of newly laid eggs treated with O₂ or HCl. Digital gene expression analysis showed that 610 genes in O₂-treated eggs and 656 in HCl-treated eggs were differentially expressed. Of these, 343 genes were differentially expressed in both treatments. In addition to trehalases, sorbic acid dehydrogenases, and some enzymes involved in the carbohydrate metabolism, we also identified heat shock proteins, cytochrome P450, and GADD45, which are related to stress tolerance. Gene ontology enrichment analysis showed differentially expressed genes in O₂-treated eggs were involved in oxidoreductase activity as well as in binding, catalytic, and metabolic processes. The Kyoto Encyclopedia of Genes and Genomes analysis showed that the pathways for ribosome biogenesis, spliceosome, and circadian rhythm were significantly enriched in HCl-treated eggs. The reliability of the data was confirmed by qRT-PCR analysis. Our results improved the understanding of the mechanism of diapause blocking in silkworm eggs treated with O₂ or HCl and identified novel molecular targets for future studies.
authors.affiliation: State Key Laboratory of Silkworm Genome Biology, College of Biotechnology, Southwest University, Chongqing 400715, China. 03gongjing@163.com.
authors.forename: Jing
authors.initials: J
authors.lastname: Gong
authors.affiliation: State Key Laboratory of Silkworm Genome Biology, College of Biotechnology, Southwest University, Chongqing 400715, China. tiansha1015@163.com.
authors.forename: Sha
authors.initials: S
authors.lastname: Tian
authors.affiliation: State Key Laboratory of Silkworm Genome Biology, College of Biotechnology, Southwest University, Chongqing 400715, China. zhouxiagz@163.com.
authors.forename: Xia
authors.initials: X
authors.lastname: Zhou
authors.affiliation: State Key Laboratory of Silkworm Genome Biology, College of Biotechnology, Southwest University, Chongqing 400715, China. m18183079489@163.com.
authors.forename: Huan
authors.initials: H
authors.lastname: Yang
authors.affiliation: State Key Laboratory of Silkworm Genome Biology, College of Biotechnology, Southwest University, Chongqing 400715, China. zhuy@swu.edu.cn.
authors.forename: Yong
authors.initials: Y
authors.lastname: Zhu
authors.affiliation: State Key Laboratory of Silkworm Genome Biology, College of Biotechnology, Southwest University, Chongqing 400715, China. yhou@swu.edu.cn.
authors.forename: Yong
authors.initials: Y
authors.lastname: Hou
mesh_terms: D000818:Animals; D012831:Bombyx / Q000187:drug effects* / Q000235:genetics*; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects / Q000235:genetics; D006851:Hydrochloric Acid / Q000494:pharmacology*; D010063:Ovum / Q000187:drug effects* / Q000378:metabolism*; D010100:Oxygen / Q000494:pharmacology*; D014198:Trehalase / Q000378:metabolism
chemical_list: D014198:Trehalase; D006851:Hydrochloric Acid; D010100:Oxygen
keywords: RNA biogenesis; diapause; heat shock protein; silkworm; trehalase