title: Characterization ofTamyb10allelic variants and development of STS marker for pre-harvest sprouting resistance in Chinese bread wheat.
abstract: Wheat grain color does not only affect the brightness of flour but also seed dormancy and pre-harvest sprouting (PHS) tolerance. The transcription factorTamyb10is an important candidate forR-1gene, and the expression of its homologs determines wheat seed coat color. In the present study, the allelic variations ofTamyb10were explored in a set of Chinese bread wheat varieties and advanced lines with different PHS tolerance, and a sequenced-tagged site (STS) marker forTamyb10-D1gene was developed, designated asTamyb10D, which could be used as an efficient and reliable marker to evaluate the depth dormancy of wheat seeds. Using the markerTamyb10D, 1629- and 1178-bp PCR fragments were amplified from the tolerant varieties, whereas a 1178-bp fragment was from the susceptible ones. Of the Chinese bread wheat varieties and advanced lines, 103 were used to validate the relationship between the polymorphic fragments ofTamyb10Dand PHS tolerance. Statistical analysis indicated thatTamyb10Dwas significantly (P< 0.001) associated with depth of seed dormancy in these germplasms. To further confirm the association between allelic variants ofTamyb10-D1and PHS tolerance, 200 recombinant inbred lines (RILs) from the cross between Zhongyou 9507 (1178-bp fragment) and Yangxiaomai (1178- and 1629-bp fragments) were genotyped using the markerTamyb10D. General linear model analysis indicated that variation inTamyb10-D1had a significant (P< 0.001) association with the germination index (GI) values, explaining 13.7, 4.7, and 9.8 % of the phenotypic variation in GI in Shijiazhuang, Beijing, and the averaged data from those environments, respectively. In addition, among the 103 wheat varieties, 8Tamyb10genotypes (Tamybl0-A1,Tamybl0-B1, andTamyb10-D1loci) were detected, namely, aaa, aab, aba, abb, baa, bab, bba, and bbb, and these were significantly associated with GI value.
authors.affiliation: College of Life Sciences, Inner Mongolia Agricultural University, 306 Zhaowuda Road, Hohhot, Inner Mongolia 010018 China.
authors.forename: Y
authors.initials: Y
authors.lastname: Wang
authors.affiliation: College of Life Sciences, Inner Mongolia Agricultural University, 306 Zhaowuda Road, Hohhot, Inner Mongolia 010018 China.
authors.forename: X L
authors.initials: XL
authors.lastname: Wang
authors.affiliation: College of Life Sciences, Inner Mongolia Agricultural University, 306 Zhaowuda Road, Hohhot, Inner Mongolia 010018 China.
authors.forename: J Y
authors.initials: JY
authors.lastname: Meng
authors.affiliation: Institute of Crop Science, National Wheat Improvement Center, Chinese Academy of Agricultural Sciences (CAAS), 12 Zhongguancun South Street, Beijing, 100081 China.
authors.forename: Y J
authors.initials: YJ
authors.lastname: Zhang
authors.affiliation: Institute of Crop Science, National Wheat Improvement Center, Chinese Academy of Agricultural Sciences (CAAS), 12 Zhongguancun South Street, Beijing, 100081 China.
authors.forename: Z H
authors.initials: ZH
authors.lastname: He
authors.affiliation: College of Life Sciences, Inner Mongolia Agricultural University, 306 Zhaowuda Road, Hohhot, Inner Mongolia 010018 China.
authors.forename: Y
authors.initials: Y
authors.lastname: Yang
keywords: Allelic variation; Germination index (GI); Tamyb10-D1; Triticum aestivum