title: Lipoprotein lipase does not increase significantly in the postprandial plasma.
abstract: BACKGROUND
  Previous reports have shown that lipoprotein lipase (LPL) activity significantly increases in the postprandial plasma associated with the increase of TG-rich lipoproteins. Therefore, we have reexamined those relationships using newly developed LPL assay with the different kinds of food intake.
  
  
  METHODS
  Standard meal (n=81), 50g of fat (n=54), 75g of glucose (n=25) and cookie (25g fat and 75g carbohydrate fat) (n=28) were administered in generally healthy volunteers. Plasma LPL, HTGL and TC, TG, LDL-C, HDL-C, RLP-C and RLP-TG were determined at subsequent withdrawal after the food intake.
  
  
  RESULTS
  Plasma TG, RLP-C and RLP-TG were significantly increased at 8PM (2h after dinner of standard meal) compared with 8AM before breakfast within the same day. Also those parameters were significantly increased in 2-6h after fat load. However, the concentrations and activities of LPL and HTGL did not significantly increase in association with an increase in the TG and remnant lipoproteins. Also LPL concentration did not significantly increase after glucose and "cookie test" within 4h.
  
  
  CONCLUSIONS
  No significant increase of LPL activity was found at CM and VLDL overload after different kinds of food intake when reexamined by newly developed assay for LPL activity and concentration.
authors.affiliation: Hidaka Hospital, Takasaki, Japan.
authors.forename: Nobuyoshi
authors.initials: N
authors.lastname: Ishiyama
authors.affiliation: Hidaka Hospital, Takasaki, Japan.
authors.forename: Kouji
authors.initials: K
authors.lastname: Sakamaki
authors.affiliation: Hidaka Hospital, Takasaki, Japan.
authors.forename: Younosuke
authors.initials: Y
authors.lastname: Shimomura
authors.affiliation: Division of Preventive Medicine, Clinical Research Institute, National Hospital Organization Kyoto Medical Center, Kyoto, Japan; Division of Community and Family Medicine, Jichi Medical University, Tochigi, Japan.
authors.forename: Kazuhiko
authors.initials: K
authors.lastname: Kotani
authors.affiliation: Division of Preventive Medicine, Clinical Research Institute, National Hospital Organization Kyoto Medical Center, Kyoto, Japan.
authors.forename: Kokoro
authors.initials: K
authors.lastname: Tsuzaki
authors.affiliation: Division of Preventive Medicine, Clinical Research Institute, National Hospital Organization Kyoto Medical Center, Kyoto, Japan.
authors.forename: Naoki
authors.initials: N
authors.lastname: Sakane
authors.affiliation: Immuno-Biological Laboratories, Fujioka, Japan.
authors.forename: Kazuya
authors.initials: K
authors.lastname: Miyashita
authors.affiliation: Immuno-Biological Laboratories, Fujioka, Japan.
authors.forename: Isao
authors.initials: I
authors.lastname: Fukamachi
authors.affiliation: Kanazawa Medical University, General Internal Medicine, Kanazawa, Japan.
authors.forename: Junji
authors.initials: J
authors.lastname: Kobayashi
authors.affiliation: Department of Molecular Biosciences, School of Veterinary Medicine and Department of Nutrition, University of California, Davis, CA, USA.
authors.forename: Kimber L
authors.initials: KL
authors.lastname: Stanhope
authors.affiliation: Department of Molecular Biosciences, School of Veterinary Medicine and Department of Nutrition, University of California, Davis, CA, USA.
authors.forename: Peter J
authors.initials: PJ
authors.lastname: Havel
authors.affiliation: Laboratory of Clinical Nutrition and Medicine, Kagawa Nutrition University, Tokyo, Japan.
authors.forename: Keiko
authors.initials: K
authors.lastname: Kamachi
authors.affiliation: Laboratory of Clinical Nutrition and Medicine, Kagawa Nutrition University, Tokyo, Japan.
authors.forename: Akira
authors.initials: A
authors.lastname: Tanaka
authors.affiliation: Department of Laboratory Sciences, Gunma University, Graduate School of Health Sciences, Maebashi, Japan.
authors.forename: Yoshiharu
authors.initials: Y
authors.lastname: Tokita
authors.affiliation: Department of Clinical Laboratory Medicine, Gunma University, Graduate School of Medicine, Maebashi, Japan.
authors.forename: Tetsuo
authors.initials: T
authors.lastname: Machida
authors.affiliation: Department of Clinical Laboratory Medicine, Gunma University, Graduate School of Medicine, Maebashi, Japan.
authors.forename: Masami
authors.initials: M
authors.lastname: Murakami
authors.affiliation: Hidaka Hospital, Takasaki, Japan; Kanazawa Medical University, General Internal Medicine, Kanazawa, Japan; Department of Molecular Biosciences, School of Veterinary Medicine and Department of Nutrition, University of California, Davis, CA, USA; Department of Clinical Laboratory Medicine, Gunma University, Graduate School of Medicine, Maebashi, Japan. Electronic address: nakajimak05@ybb.ne.jp.
authors.forename: Katsuyuki
authors.initials: K
authors.lastname: Nakajima
mesh_terms: D000293:Adolescent; D000328:Adult; D004040:Dietary Carbohydrates / Q000494:pharmacology; D004041:Dietary Fats / Q000494:pharmacology; D004347:Drug Interactions; D004435:Eating / Q000187:drug effects; D005215:Fasting / Q000097:blood; D005260:Female; D006801:Humans; D008049:Lipase / Q000097:blood; D008071:Lipoprotein Lipase / Q000097:blood*; D008297:Male; D019518:Postprandial Period / Q000187:drug effects; D055815:Young Adult
chemical_list: D004040:Dietary Carbohydrates; D004041:Dietary Fats; C504173:LIPC protein, human; D008049:Lipase; D008071:Lipoprotein Lipase
keywords: Chylomicrons; Hepatic triglyceride lipase; Lipoprotein lipase; RLP-C; RLP-TG; Remnant lipoproteins; Triglycerides; Very low density lipoproteins