title: Genomic studies of envelope gene sequences from mosquito and human samples from Bangkok, Thailand.
abstract: Dengue virus (DENV) is an RNA virus showing a high degree of genetic variation as a consequence of its proofreading inability. This variation plays an important role in virus evolution and pathogenesis. Although levels of within-host genetic variation are similar following equilibrium, variation among different hosts is frequently different. To identify dengue quasispecies present among two hosts, we collected patient samples from six acute DENV cases and two pools ofAedes aegyptimosquitoes and analyzed the genetic variation of regions of the viral envelope gene. Among human and mosquito samples, we found three major clusters originating from two subpopulations. Although several shared lineages were observed in the two hosts, only one lineage showing evidence of neutral selection was observed among two hosts. Taken together, our data provide evidence for the existence of a DENV quasispecies, with less genetic variation observed in mosquitoes than humans and with circulating lineages found in both host types.
authors.affiliation: Center of Excellence for Antibody Research, Faculty of Tropical Medicine, Mahidol University, 420/6 Ratchathewi, Bangkok, 10400 Thailand ; Department of Social and Environmental Medicine, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand.
authors.forename: Pannamthip
authors.initials: P
authors.lastname: Pitaksajjakul
authors.affiliation: Center of Excellence for Antibody Research, Faculty of Tropical Medicine, Mahidol University, 420/6 Ratchathewi, Bangkok, 10400 Thailand.
authors.forename: Surachet
authors.initials: S
authors.lastname: Benjathummarak
authors.affiliation: Department of Social and Environmental Medicine, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand.
authors.forename: Hyun Ngoc
authors.initials: HN
authors.lastname: Son
authors.affiliation: Department of Medical Entomology, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand.
authors.forename: Supatra
authors.initials: S
authors.lastname: Thongrungkiat
authors.affiliation: Center of Excellence for Antibody Research, Faculty of Tropical Medicine, Mahidol University, 420/6 Ratchathewi, Bangkok, 10400 Thailand ; Department of Social and Environmental Medicine, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand.
authors.forename: Pongrama
authors.identifier: 0000-0003-3097-5733
authors.initials: P
authors.lastname: Ramasoota
keywords: Bangkok; Dengue virus serotype 2; Human; Mosquitoes; Quasispecies; Thailand