title: Scaling-up and ionic liquid-based extraction of pectinases from Aspergillus flavipes cultures.
abstract: The viability of the scaling-up of pectinases production by Aspergillus flavipes at 5L-bioreactor scale has been demonstrated by keeping constant the power input, and a drastic increase in the endo- and exopectinolytic enzyme production was recorded (7- and 40-fold, respectively). The main process variables were modelled by means of logistic and Gompertz equations. In order to overcome the limitations of the conventional downstream strategies, a novel extraction strategy was proposed on the basis of the adequate salting-out potential of two biocompatible cholinium-based ionic liquids (N1112OHCl and N1112OHH2PO4) in aqueous solutions of Tergitol, reaching more than 90% of extraction.
authors.affiliation: División de Ingeniería Química y Bioquímica, Tecnológico de Estudios Superiores de Ecatepec, CP 55210 México D.F., Mexico; Departamento de Ingeniería Química, Campus Lagoas Marcosende, Universidad de Vigo, Vigo 36310, Spain.
authors.forename: Vicente E
authors.initials: VE
authors.lastname: Wolf-Márquez
authors.affiliation: División de Ingeniería Química y Bioquímica, Tecnológico de Estudios Superiores de Ecatepec, CP 55210 México D.F., Mexico.
authors.forename: M Aurora
authors.initials: MA
authors.lastname: Martínez-Trujillo
authors.affiliation: Grupo de Fisiología de Hongos, Departamento de Alimentos y Biotecnología, Universidad Nacional Autónoma de México, CP 04510 México, D.F, Mexico.
authors.forename: Guillermo
authors.initials: G
authors.lastname: Aguilar Osorio
authors.affiliation: Departamento de Diseño en la Ingeniería, Campus Lagoas Marcosende, Universidad de Vigo, Vigo 36310, Spain.
authors.forename: Faustino
authors.initials: F
authors.lastname: Patiño
authors.affiliation: Departamento de Ingeniería Química, Campus Lagoas Marcosende, Universidad de Vigo, Vigo 36310, Spain.
authors.forename: María S
authors.initials: MS
authors.lastname: Álvarez
authors.affiliation: Departamento de Ingeniería Química, Campus Lagoas Marcosende, Universidad de Vigo, Vigo 36310, Spain. Electronic address: aroguez@uvigo.es.
authors.forename: Ana
authors.initials: A
authors.lastname: Rodríguez
authors.affiliation: Departamento de Ingeniería Química, Campus Lagoas Marcosende, Universidad de Vigo, Vigo 36310, Spain.
authors.forename: M Ángeles
authors.initials: MÁ
authors.lastname: Sanromán
authors.affiliation: Departamento de Ingeniería Química, Campus Lagoas Marcosende, Universidad de Vigo, Vigo 36310, Spain.
authors.forename: Francisco J
authors.initials: FJ
authors.lastname: Deive
mesh_terms: D001230:Aspergillus / Q000737:chemistry* / Q000378:metabolism; D019149:Bioreactors; D005591:Chemical Fractionation / Q000379:methods; D007218:Industrial Microbiology / Q000295:instrumentation / Q000379:methods*; D052578:Ionic Liquids / Q000737:chemistry*; D011060:Poloxalene / Q000737:chemistry; D011096:Polygalacturonase / Q000302:isolation & purification* / Q000378:metabolism*
chemical_list: D052578:Ionic Liquids; D011060:Poloxalene; D011096:Polygalacturonase
keywords: Aqueous Biphasic Systems; Bioreactor; Cholinium; Ionic liquids; Pectinolytic enzymes