title: Plasmonic nanoshells enhanced laser desorption/ionization mass spectrometry for detection of serum metabolites.
abstract: Laser desorption/ionization mass spectrometry (LDI MS)-based small metabolites detection is fundamentally important for the clinic prognoses and diagnoses. Plasmonic materials have been applied as efficient substrates for LDI MS of these molecules. However, there is no clear understanding of the mechanism of using plasmonic materials for enhanced LDI MS of small metabolites, thus restricting their application for real case serum samples. In this work, we report the use of plasmonic nanoshells for enhanced LDI MS detection of small analytes. The antibonding modes of Au nanoshells provided unique opportunity for the generation of hot carriers in the ultra-violet (UV) optical range. This effect of the Au nanoshells displayed desirable analytical sensitivity and endurance towards small metabolites (e.g. amino acids) in complex protein/mixtures as compared to Au nanorods/nanospheres and conventional organic matrix. Further we achieved LDI MS profiling and detection of small metabolites from complex serum samples by Au nanoshells. Our work not only shed light on the enhancement mechanism of LDI MS detection using plasmonic nanoparticles with hot carriers, but also contributed to the real case application of LDI MS in clinical study.
authors.affiliation: School of Biomedical Engineering and Med-X Research Institute, Shanghai Jiao Tong University, Shanghai 200030, P.R. China.
authors.forename: Xiang
authors.initials: X
authors.lastname: Wei
authors.affiliation: School of Biomedical Engineering and Med-X Research Institute, Shanghai Jiao Tong University, Shanghai 200030, P.R. China.
authors.forename: Zhonghui
authors.initials: Z
authors.lastname: Liu
authors.affiliation: School of Biomedical Engineering and Med-X Research Institute, Shanghai Jiao Tong University, Shanghai 200030, P.R. China.
authors.forename: Xiulong
authors.initials: X
authors.lastname: Jin
authors.affiliation: School of Biomedical Engineering and Med-X Research Institute, Shanghai Jiao Tong University, Shanghai 200030, P.R. China.
authors.forename: Lin
authors.initials: L
authors.lastname: Huang
authors.affiliation: School of Biomedical Engineering and Med-X Research Institute, Shanghai Jiao Tong University, Shanghai 200030, P.R. China.
authors.forename: Deepanjali Dattatray
authors.initials: DD
authors.lastname: Gurav
authors.affiliation: School of Biomedical Engineering and Med-X Research Institute, Shanghai Jiao Tong University, Shanghai 200030, P.R. China.
authors.forename: Xuming
authors.initials: X
authors.lastname: Sun
authors.affiliation: Department of Chemistry, Institute of Biomedical Sciences and State Key Lab of Molecular Engineering of Polymers, Fudan University, Shanghai 200433, P.R. China.
authors.forename: Baohong
authors.initials: B
authors.lastname: Liu
authors.affiliation: School of Biomedical Engineering and Med-X Research Institute, Shanghai Jiao Tong University, Shanghai 200030, P.R. China. Electronic address: yejian78@sjtu.edu.cn.
authors.forename: Jian
authors.initials: J
authors.lastname: Ye
authors.affiliation: School of Biomedical Engineering and Med-X Research Institute, Shanghai Jiao Tong University, Shanghai 200030, P.R. China. Electronic address: k.qian@sjtu.edu.cn.
authors.forename: Kun
authors.initials: K
authors.lastname: Qian
mesh_terms: D000596:Amino Acids / Q000032:analysis*; D006801:Humans; D057145:Nanoshells; D044967:Serum / Q000737:chemistry*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013053:Spectrophotometry
chemical_list: D000596:Amino Acids
keywords: Hot carriers; Laser desorption/ionization mass spectrometry; Metabolites; Plasmonic nanoparticles; Serum