title: Cardiac Gene Delivery in Large Animal Models: Antegrade Techniques.
abstract: Percutaneous antegrade coronary injection is among the least invasive cardiac selective gene delivery methods. However, transduction efficiency is quite low with a simple bolus antegrade injection. In order to improve the transduction efficiency using antegrade delivery, several additional approaches have been proposed.In this chapter, we briefly discuss important elements associated with intracoronary delivery methods and present protocols for three different catheter-based antegrade delivery techniques in a preclinical large animal model. Despite the lower transduction efficacy relative to more invasive delivery techniques, antegrade techniques have the advantage of being clinically well established and having safer profiles which is important when treating patients with cardiac disease.
authors.affiliation: Cardiovascular Research Center, Icahn School of Medicine at Mount Sinai, One Gustave L. Levy Place, Box 1030, New York, NY, 10029-6574, USA.
authors.forename: Shin
authors.initials: S
authors.lastname: Watanabe
authors.affiliation: Cardiovascular Research Center, Icahn School of Medicine at Mount Sinai, One Gustave L. Levy Place, Box 1030, New York, NY, 10029-6574, USA.
authors.forename: Lauren
authors.initials: L
authors.lastname: Leonardson
authors.affiliation: Cardiovascular Research Center, Icahn School of Medicine at Mount Sinai, One Gustave L. Levy Place, Box 1030, New York, NY, 10029-6574, USA.
authors.forename: Roger J
authors.initials: RJ
authors.lastname: Hajjar
authors.affiliation: Cardiovascular Research Center, Icahn School of Medicine at Mount Sinai, One Gustave L. Levy Place, Box 1030, New York, NY, 10029-6574, USA. kiyotake.ishikawa@mssm.edu.
authors.forename: Kiyotake
authors.initials: K
authors.lastname: Ishikawa
mesh_terms: D000818:Animals; D003331:Coronary Vessels / Q000473:pathology; D018014:Gene Transfer Techniques; D023421:Models, Animal; D009206:Myocardium / Q000378:metabolism*; D010477:Perfusion; D034421:Sus scrofa
keywords: Balloon occlusion; Cardiovascular diseases; Gene delivery; Gene therapy; Homogenous distribution; Intracoronary; Minimally invasive; Vectors