title: Localized conformational interrogation of antibody and antibody-drug conjugates by site-specific carboxyl group footprinting.
abstract: Establishing and maintaining conformational integrity of monoclonal antibodies (mAbs) and antibody-drug conjugates (ADCs) during development and manufacturing is critical for ensuring their clinical efficacy. As presented here, we applied site-specific carboxyl group footprinting (CGF) for localized conformational interrogation of mAbs. The approach relies on covalent labeling that introduces glycine ethyl ester tags onto solvent-accessible side chains of protein carboxylates. Peptide mapping is used to monitor the labeling kinetics of carboxyl residues and the labeling kinetics reflects the conformation or solvent-accessibility of side chains. Our results for two case studies are shown here. The first study was aimed at defining the conformational changes of mAbs induced by deglycosylation. We found that two residues in CH2 domain (D268 and E297) show significantly enhanced side chain accessibility upon deglycosylation. This site-specific result highlighted the advantage of monitoring the labeling kinetics at the amino acid level as opposed to the peptide level, which would result in averaging out of highly localized conformational differences. The second study was designed to assess conformational effects brought on by conjugation of mAbs with drug-linkers. All 59 monitored carboxyl residues displayed similar solvent-accessibility between the ADC and mAb under native conditions, which suggests the ADC and mAb share similar side chain conformation. The findings are well correlated and complementary with results from other assays. This work illustrated that site-specific CGF is capable of pinpointing local conformational changes in mAbs or ADCs that might arise during development and manufacturing. The methodology can be readily implemented within the industry to provide comprehensive conformational assessment of these molecules.
authors.affiliation: a Seattle Genetics, Inc. , Bothell , WA , USA.
authors.forename: Lucy Yan
authors.initials: LY
authors.lastname: Pan
authors.affiliation: a Seattle Genetics, Inc. , Bothell , WA , USA.
authors.forename: Oscar
authors.initials: O
authors.lastname: Salas-Solano
authors.affiliation: a Seattle Genetics, Inc. , Bothell , WA , USA.
authors.forename: John F
authors.initials: JF
authors.lastname: Valliere-Douglass
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis / Q000737:chemistry*; D041961:Deuterium Exchange Measurement / Q000379:methods*; D006801:Humans; D018796:Immunoconjugates / Q000032:analysis / Q000737:chemistry*; D007700:Kinetics; D013058:Mass Spectrometry / Q000379:methods*; D015202:Protein Engineering
chemical_list: D000911:Antibodies, Monoclonal; D018796:Immunoconjugates
keywords: Antibody-drug conjugate; Protein conformation; carboxyl group footprinting; covalent labeling; hydrogen-deuterium exchange; mass spectrometry; monoclonal antibody; rate constant; side chains; solvent accessibility