title: The effect of the additional cytogenetic abnormalities on major molecular response and BCR-ABL kinase domain mutations in long-term follow-up chronic myeloid leukemia patients, a cross sectional study.
abstract: The aim of the study was to examine the relation between additional chromosomal aberrations (ACAs) with major molecular response (MMR) and BCR-ABL kinase domain (KD) mutations in the long-term follow-up of the chronic myeloid leukemia (CML) disease. The study design was cross-sectional observational and used the CML patients' data of Izmir Ataturk Education and Research Hospital from 2011 to 2015. Conventional cytogenetic, fluorescence in situ hybridization (FISH), quantitative real-time polymerase chain reaction (RQ-PCR) test results from 89 CML patients' and pyrosequencing analysis results from 17 patients' were set up for comparison analysis. The chi-square test was used in statistical analysis of the experimental data. There were no statistically significant correlations between ACAs and MMR (p = .361, p > .05) groups or BCR-ABL KD mutations (p = .576, p > .05) groups observed in the study. This study has revealed that MMR and BCR-ABL KD mutations did not correlate with ACAs.
authors.affiliation: a Department of Genetic , Izmir Ataturk Education and Research Hospital , Izmir , Turkey.
authors.forename: Kaan
authors.initials: K
authors.lastname: Savasoglu
authors.affiliation: b Department of Hematology , Izmir Ataturk Education and Research Hospital , Izmir , Turkey.
authors.forename: Kadriye Bahriye
authors.initials: KB
authors.lastname: Payzin
authors.affiliation: b Department of Hematology , Izmir Ataturk Education and Research Hospital , Izmir , Turkey.
authors.forename: Fusun
authors.initials: F
authors.lastname: Ozdemirkiran
authors.affiliation: a Department of Genetic , Izmir Ataturk Education and Research Hospital , Izmir , Turkey.
authors.forename: Asli
authors.initials: A
authors.lastname: Subasioglu
authors.affiliation: b Department of Hematology , Izmir Ataturk Education and Research Hospital , Izmir , Turkey.
authors.forename: Asu Fergun
authors.initials: AF
authors.lastname: Yilmaz
mesh_terms: D000328:Adult; D000368:Aged; D002869:Chromosome Aberrations; D003430:Cross-Sectional Studies; D019008:Drug Resistance, Neoplasm / Q000235:genetics; D005260:Female; D005500:Follow-Up Studies; D016044:Fusion Proteins, bcr-abl / Q000235:genetics*; D006801:Humans; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000188:drug therapy / Q000235:genetics* / Q000401:mortality / Q000473:pathology; D008297:Male; D008875:Middle Aged; D009154:Mutation; D009367:Neoplasm Staging; D011379:Prognosis; D054730:Protein Interaction Domains and Motifs / Q000235:genetics*; D047428:Protein Kinase Inhibitors / Q000494:pharmacology / Q000627:therapeutic use; D016896:Treatment Outcome
chemical_list: D047428:Protein Kinase Inhibitors; D016044:Fusion Proteins, bcr-abl
keywords: CML; TKI; cytogenetic abnormalities; prognosis; quantitative real-time PCR