title: Evaluating Aflatoxin Gene Expression in Aspergillus Section Flavi.
abstract: The determination of aflatoxin production ability and differentiation of aflatoxigenic strains can be assessed by monitoring the expression of one or several key genes using reverse transcription polymerase chain reaction (RT-PCR). We herein describe the methods for RNA induction, extraction, and quality determination, and the RT-PCR conditions used to evaluate the ability of a given Aspergillus strain to produce aflatoxins.
authors.affiliation: CIMO/School of Agriculture, The Polytechnic Institute of Bragança, Campus de Santa Apolónia, 5300-253, Bragança, Portugal. prodrigues@ipb.pt.
authors.forename: Paula Cristina Azevedo
authors.initials: PC
authors.lastname: Rodrigues
authors.affiliation: Facultad de Ciencias Biologicas, Departmento de Microbiologia III, Universidad Complutense de Madrid, Jose Antonio Novais 12, E-28040, Madrid, Spain.
authors.forename: Jéssica
authors.initials: J
authors.lastname: Gil-Serna
authors.affiliation: Facultad de Ciencias Biologicas, Departmento de Genética, Universidad Complutense de Madrid, Jose Antonio Novais 12, E-28040, Madrid, Spain.
authors.forename: M Teresa
authors.initials: MT
authors.lastname: González-Jaén
mesh_terms: D000348:Aflatoxins / Q000096:biosynthesis / Q000235:genetics*; D001231:Aspergillus flavus / Q000235:genetics* / Q000378:metabolism; D015966:Gene Expression Regulation, Fungal; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D000348:Aflatoxins
keywords: Aflatoxigenic fungi; Aspergillus flavus; Gel electrophoresis; Mycotoxins; RNA extraction; RT-PCR