title: Photo-Modulated Therapeutic Protein Release from a Hydrogel Depot Using Visible Light.
abstract: The use of biomacromolecular therapeutics has revolutionized disease treatment, but frequent injections are required owing to their short half-life in vivo. Thus there is a need for a drug delivery system that acts as a reservoir and releases the drug remotely "on demand". Here we demonstrate a simple light-triggered local drug delivery system through photo-thermal interactions of polymer-coated gold nanoparticles (AuNPs) inside an agarose hydrogel as therapeutic depot. Localized temperature increase induced by the visible light exposure caused reversible softening of the hydrogel matrix to release the pre-loaded therapeutics. The release profile can be adjusted by AuNPs and agarose concentrations, light intensity and exposure time. Importantly, the biological activity of the released bevacizumab was highly retained. In this study we demonstrate the potential application of this facile AuNPs/hydrogel system for ocular therapeutics delivery through its versatility to release multiple biologics, compatibility to ocular cells and spatiotemporal control using visible light.
authors.affiliation: CSIRO Manufacturing, Bayview Avenue, Clayton, Victoria, Australia.
authors.forename: Johan S
authors.identifier: 0000-0002-1620-2198
authors.initials: JS
authors.lastname: Basuki
authors.affiliation: Beijing Key Lab of Bioprocess, Beijing University of Chemical Technology, Beijing, P.R. China.
authors.forename: Fengxiang
authors.initials: F
authors.lastname: Qie
authors.affiliation: CSIRO Manufacturing, Bayview Avenue, Clayton, Victoria, Australia.
authors.forename: Xavier
authors.initials: X
authors.lastname: Mulet
authors.affiliation: CSIRO Manufacturing, Bayview Avenue, Clayton, Victoria, Australia.
authors.forename: Randy
authors.initials: R
authors.lastname: Suryadinata
authors.affiliation: CSIRO Manufacturing, Bayview Avenue, Clayton, Victoria, Australia.
authors.forename: Aditya V
authors.initials: AV
authors.lastname: Vashi
authors.affiliation: CSIRO Manufacturing, Bayview Avenue, Clayton, Victoria, Australia.
authors.forename: Yong Y
authors.initials: YY
authors.lastname: Peng
authors.affiliation: School of Ophthalmology & Optometry and Eye Hospital, Wenzhou Medical University, Wenzhou, P.R. China.
authors.forename: Lingli
authors.initials: L
authors.lastname: Li
authors.affiliation: CSIRO Manufacturing, Bayview Avenue, Clayton, Victoria, Australia.
authors.forename: Xiaojuan
authors.initials: X
authors.lastname: Hao
authors.affiliation: Beijing Key Lab of Bioprocess, Beijing University of Chemical Technology, Beijing, P.R. China.
authors.forename: Tianwei
authors.initials: T
authors.lastname: Tan
authors.affiliation: CSIRO Manufacturing, Bayview Avenue, Clayton, Victoria, Australia.
authors.forename: Timothy C
authors.identifier: 0000-0002-7990-4509
authors.initials: TC
authors.lastname: Hughes
mesh_terms: D016503:Drug Delivery Systems; D006046:Gold / Q000737:chemistry; D006801:Humans; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000737:chemistry*; D008027:Light; D053768:Metal Nanoparticles / Q000737:chemistry; D010316:Particle Size; D010778:Photochemotherapy; D011108:Polymers / Q000737:chemistry; D011506:Proteins / Q000737:chemistry*; D013499:Surface Properties
chemical_list: D011108:Polymers; D011506:Proteins; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D006046:Gold
keywords: agarose; drug delivery; gold nanoparticles; macular degeneration; photothermal effect