title: Precursor processing for plant peptide hormone maturation by subtilisin-like serine proteinases.
abstract: Peptide hormones that regulate plant growth and development are derived from larger precursor proteins by proteolytic processing. Our study addressed the role of subtilisin-like proteinases (SBTs) in this process. Using tissue-specific expression of proteinase inhibitors as a tool to overcome functional redundancy, we found that SBT activity was required for the maturation of IDA (INFLORESCENCE DEFICIENT IN ABSCISSION), a peptide signal for the abscission of floral organs in Arabidopsis We identified three SBTs that process the IDA precursor in vitro, and this processing was shown to be required for the formation of mIDA (the mature and bioactive form of IDA) as the endogenous signaling peptide in vivo. Hence, SBTs act as prohormone convertases in plants, and several functionally redundant SBTs contribute to signal biogenesis.
authors.affiliation: University of Hohenheim, Institute of Plant Physiology and Biotechnology, 70593 Stuttgart, Germany.
authors.forename: Katharina
authors.initials: K
authors.lastname: Schardon
authors.affiliation: University of Hohenheim, Institute of Plant Physiology and Biotechnology, 70593 Stuttgart, Germany.
authors.forename: Mathias
authors.initials: M
authors.lastname: Hohl
authors.affiliation: University of Hohenheim, Institute of Plant Physiology and Biotechnology, 70593 Stuttgart, Germany.
authors.forename: Lucile
authors.initials: L
authors.lastname: Graff
authors.affiliation: Core Facility Hohenheim, Mass Spectrometry Unit, 70593 Stuttgart, Germany.
authors.forename: Jens
authors.initials: J
authors.lastname: Pfannstiel
authors.affiliation: University of Hohenheim, Department of Plant Systems Biology, 70593 Stuttgart, Germany.
authors.forename: Waltraud
authors.initials: W
authors.lastname: Schulze
authors.affiliation: University of Hohenheim, Institute of Plant Physiology and Biotechnology, 70593 Stuttgart, Germany.
authors.forename: Annick
authors.initials: A
authors.lastname: Stintzi
authors.affiliation: University of Hohenheim, Institute of Plant Physiology and Biotechnology, 70593 Stuttgart, Germany. andreas.schaller@uni-hohenheim.de.
authors.forename: Andreas
authors.initials: A
authors.lastname: Schaller
mesh_terms: D017360:Arabidopsis / Q000201:enzymology / Q000254:growth & development / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000378:metabolism*; D035264:Flowers / Q000201:enzymology / Q000378:metabolism; D036361:Peptide Hormones / Q000378:metabolism*; D010937:Plant Growth Regulators / Q000378:metabolism*; D043484:Proprotein Convertases / Q000378:metabolism*; D011499:Protein Processing, Post-Translational; D021382:Protein Sorting Signals; D059748:Proteolysis; D013381:Subtilisins / Q000378:metabolism*
chemical_list: D029681:Arabidopsis Proteins; C512213:IDA protein, Arabidopsis; D036361:Peptide Hormones; D010937:Plant Growth Regulators; D021382:Protein Sorting Signals; D043484:Proprotein Convertases; D013381:Subtilisins