title: miR-125b Enhances IL-8 Production in Early-Onset Severe Preeclampsia by Targeting Sphingosine-1-Phosphate Lyase 1.
abstract: Preeclampsia (PE) is one of the leading causes of maternal and perinatal mortality and morbidity. One of the main hallmarks observed in PE is impaired inflammation state. In the current study, we found that miR-125b was deregulated in placental tissues and plasma derived from PE patients, which suggest a potential association between this miRNA and the pathogenesis of PE. Overexpression of miR-125b significantly reduced SGPL1 expression, and luciferase assays confirmed that SGPL1 is a direct target of miR-125b. We also found that miR-125b enhanced IL-8 production by directly targeting sphingosine-1-phosphate lyase 1 (SGPL1), and this effect could be reversed by SGPL1 overexpression. In placentas derived from PE patients, a negative correlation of miR-125b and SGPL1 was observed, and IL-8 was validated to be increased in the circulation of PE patients. Our data demonstrated a critical role of miR-125b in IL-8 production and the development of PE.
authors.affiliation: School of Biological Sciences, Weifang Medical University, Weifang, China.|Biopharmaceutical Laboratory of Health and Family Planning Commission of Shandong Province, Weifang Medical University, Weifang, China.
authors.forename: Weiwei
authors.initials: W
authors.lastname: Yang
authors.affiliation: Department of Cardiology, Weifang People's Hospital, Weifang, China.
authors.forename: Anning
authors.initials: A
authors.lastname: Wang
authors.affiliation: School of Biological Sciences, Weifang Medical University, Weifang, China.|Biopharmaceutical Laboratory of Health and Family Planning Commission of Shandong Province, Weifang Medical University, Weifang, China.
authors.forename: Chunling
authors.initials: C
authors.lastname: Zhao
authors.affiliation: Public Health College, Weifang Medical University, Weifang, China.
authors.forename: Qinghua
authors.initials: Q
authors.lastname: Li
authors.affiliation: School of Biological Sciences, Weifang Medical University, Weifang, China.
authors.forename: Zhifang
authors.initials: Z
authors.lastname: Pan
authors.affiliation: Department of Cardiology, Weifang People's Hospital, Weifang, China.
authors.forename: Xuefu
authors.initials: X
authors.lastname: Han
authors.affiliation: Department of Obstetrics, Affiliated Hospital of Weifang Medical University, Weifang, China.
authors.forename: Cuijuan
authors.initials: C
authors.lastname: Zhang
authors.affiliation: School of Biological Sciences, Weifang Medical University, Weifang, China.
authors.forename: Guohui
authors.initials: G
authors.lastname: Wang
authors.affiliation: School of Biological Sciences, Weifang Medical University, Weifang, China.
authors.forename: Chao
authors.initials: C
authors.lastname: Ji
authors.affiliation: Center for Reproductive Medicine, Affiliated Hospital of Weifang Medical University, Weifang, China.
authors.forename: Guili
authors.initials: G
authors.lastname: Wang
authors.affiliation: School of Biological Sciences, Weifang Medical University, Weifang, China.
authors.forename: Guangtao
authors.initials: G
authors.lastname: Jia
authors.affiliation: School of Biological Sciences, Weifang Medical University, Weifang, China.
authors.forename: Jiyu
authors.initials: J
authors.lastname: Ju
authors.affiliation: School of Biological Sciences, Weifang Medical University, Weifang, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Gao
authors.affiliation: School of Biological Sciences, Weifang Medical University, Weifang, China.
authors.forename: Wenjing
authors.initials: W
authors.lastname: Yu
authors.affiliation: School of Biological Sciences, Weifang Medical University, Weifang, China.
authors.forename: Xiaoying
authors.initials: X
authors.lastname: Liu
authors.affiliation: Department of Dentistry, Weifang Maternity and Child Care Hospital, Weifang, China.
authors.forename: Xi
authors.initials: X
authors.lastname: Chen
authors.affiliation: School of Biological Sciences, Weifang Medical University, Weifang, China.
authors.forename: Weiguo
authors.initials: W
authors.lastname: Feng
authors.affiliation: School of Biological Sciences, Weifang Medical University, Weifang, China.|Biopharmaceutical Laboratory of Health and Family Planning Commission of Shandong Province, Weifang Medical University, Weifang, China.
authors.forename: Zhiqin
authors.initials: Z
authors.lastname: Gao
authors.affiliation: Department of Obstetrics, Affiliated Hospital of Weifang Medical University, Weifang, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Li
authors.affiliation: Center for Reproductive Medicine, Affiliated Hospital of Weifang Medical University, Weifang, China.
authors.forename: Chune
authors.initials: C
authors.lastname: Ren
mesh_terms: D020413:3' Untranslated Regions / Q000235:genetics; D000328:Adult; D000446:Aldehyde-Lyases / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D015153:Blotting, Western; D002460:Cell Line; D005260:Female; D005786:Gene Expression Regulation; D006801:Humans; D007150:Immunohistochemistry; D016209:Interleukin-8 / Q000096:biosynthesis* / Q000097:blood; D035683:MicroRNAs / Q000097:blood / Q000235:genetics*; D011225:Pre-Eclampsia / Q000097:blood / Q000235:genetics* / Q000378:metabolism; D011247:Pregnancy; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012689:Sequence Homology, Nucleic Acid; D055815:Young Adult
chemical_list: D020413:3' Untranslated Regions; D016209:Interleukin-8; C531888:MIRN125 microRNA, human; D035683:MicroRNAs; D000446:Aldehyde-Lyases; C022649:sphingosine 1-phosphate lyase (aldolase)