title: Photocaged G-Quadruplex DNAzyme and Aptamer by Post-Synthetic Modification on Phosphodiester Backbone.
abstract: G-quadruplex-containing DNAzymes and aptamers are widely applied in many research fields because of their high stability and prominent activities versus the protein counterparts. In this work, G-quadruplex DNAs were equipped with photolabile groups to construct photocaged DNAzymes and aptamers. We incorporated TEEP-OH (thioether-enol phosphate, phenol substituted) into phosphodiester backbone of G-quadruplex DNA by a facile post-synthetic method to achieve efficient photocaging of their activities. Upon light irradiation, the peroxidase-mimicking activity of the caged G-quadruplex DNAzyme was activated, through the transformation of TEEP-OH into a native DNA phosphodiester without any artificial scar. Similarly, the caged G-quadruplex thrombin-binding aptamer also showed light-induced activation of thrombin inhibition activity. This method could serve as a general strategy to prepare photocaged G-quadruplex DNA with other activities for noninvasive control of their functions.
authors.affiliation: Department of Chemistry, Beijing Key Laboratory for Microanalytical Methods and Instrumentation, Key Laboratory of Bioorganic Phosphorus Chemistry and Chemical Biology (Ministry of Education), Tsinghua University , Beijing 100084, China.
authors.forename: Mengli
authors.initials: M
authors.lastname: Feng
authors.affiliation: Department of Chemistry, Beijing Key Laboratory for Microanalytical Methods and Instrumentation, Key Laboratory of Bioorganic Phosphorus Chemistry and Chemical Biology (Ministry of Education), Tsinghua University , Beijing 100084, China.
authors.forename: Zhiyuan
authors.initials: Z
authors.lastname: Ruan
authors.affiliation: Department of Chemistry, Beijing Key Laboratory for Microanalytical Methods and Instrumentation, Key Laboratory of Bioorganic Phosphorus Chemistry and Chemical Biology (Ministry of Education), Tsinghua University , Beijing 100084, China.
authors.forename: Jiachen
authors.initials: J
authors.lastname: Shang
authors.affiliation: Department of Chemistry, Beijing Key Laboratory for Microanalytical Methods and Instrumentation, Key Laboratory of Bioorganic Phosphorus Chemistry and Chemical Biology (Ministry of Education), Tsinghua University , Beijing 100084, China.
authors.forename: Lu
authors.initials: L
authors.lastname: Xiao
authors.affiliation: Department of Chemistry, Beijing Key Laboratory for Microanalytical Methods and Instrumentation, Key Laboratory of Bioorganic Phosphorus Chemistry and Chemical Biology (Ministry of Education), Tsinghua University , Beijing 100084, China.
authors.forename: Aijun
authors.initials: A
authors.lastname: Tong
authors.affiliation: Department of Chemistry, Beijing Key Laboratory for Microanalytical Methods and Instrumentation, Key Laboratory of Bioorganic Phosphorus Chemistry and Chemical Biology (Ministry of Education), Tsinghua University , Beijing 100084, China.
authors.forename: Yu
authors.identifier: 0000-0002-5593-5773
authors.initials: Y
authors.lastname: Xiang
mesh_terms: D000991:Antithrombins / Q000737:chemistry* / Q000494:pharmacology; D052157:Aptamers, Nucleotide / Q000737:chemistry* / Q000494:pharmacology; D021881:DNA, Catalytic / Q000737:chemistry*; D004789:Enzyme Activation / Q000528:radiation effects*; D054856:G-Quadruplexes / Q000528:radiation effects*; D006801:Humans; D008027:Light; D008958:Models, Molecular; D009195:Peroxidase / Q000737:chemistry*; D010636:Phenols / Q000737:chemistry; D010710:Phosphates / Q000737:chemistry; D013440:Sulfides / Q000737:chemistry; D013917:Thrombin / Q000378:metabolism
chemical_list: D000991:Antithrombins; D052157:Aptamers, Nucleotide; D021881:DNA, Catalytic; D010636:Phenols; D010710:Phosphates; D013440:Sulfides; C081676:thrombin aptamer; D009195:Peroxidase; D013917:Thrombin