title: Preparation and characterization of polymeric nanoparticles surface modified with chitosan for target treatment of colorectal cancer.
abstract: 5-Fluorouracil (5-FU) loaded chitosan (C) coated polylactic-co-glycolic acid (PLGA) nanoparticles [NPs] (C-5-FU PLGA NPs) and polycaprolactone [PCL] (C-5-FU PCL NPs) were employed as the carriers for cancer treatment. The prepared NPs showed the spherical shape of NPs with the particle size in the range of 188.1-302.2nm with polydispersity index (PDI) of <0.30. C-coated NPs converted zeta potential from negative to positive value with small modification in particle size distribution. The entrapment efficiency of 5-FU was recorded in the range of 32-51%. The in vitro release studies showed an initial rapid 5-FU release followed by a sustained release profile. The in vitro cytotoxicity of C-5-FU PLGA NPs showed significant inhibition of colon cancer cells (HT-29) compared to the other NPs and drug solution. These results showed that C-5-FU PLGA NPs can be considered as a promising carrier for cancer therapy.
authors.affiliation: Department of Pharmaceutics, College of Pharmacy, King Saud University, P.O. Box 2457, Riyadh 11451, Saudi Arabia; Department of Pharmaceutics and Industrial Pharmacy, Faculty of Pharmacy, Al-Azhar University, Cairo, Egypt.
authors.forename: Mohamed M
authors.initials: MM
authors.lastname: Badran
authors.affiliation: Department of Physics and Astronomy, College of Science, King Saud University, Riyadh 11451, Saudi Arabia; Biophysics Department, Faculty of Science, Cairo University, Giza, Egypt.
authors.forename: Mohsen M
authors.initials: MM
authors.lastname: Mady
authors.affiliation: Department of Physics and Astronomy, College of Science, King Saud University, Riyadh 11451, Saudi Arabia; Biophysics Department, Faculty of Science, Cairo University, Giza, Egypt.
authors.forename: Magdy M
authors.initials: MM
authors.lastname: Ghannam
authors.affiliation: Department of Pharmaceutics, College of Pharmacy, King Saud University, P.O. Box 2457, Riyadh 11451, Saudi Arabia; Center of Excellence in Biotechnology Research (CEBR), King Saud University, P.O. Box 2460, Riyadh 11451, Saudi Arabia. Electronic address: faiyazs@fastmail.fm.
authors.forename: Faiyaz
authors.initials: F
authors.lastname: Shakeel
mesh_terms: D048271:Chitosan / Q000737:chemistry*; D015179:Colorectal Neoplasms / Q000188:drug therapy* / Q000473:pathology; D065546:Drug Liberation; D005472:Fluorouracil / Q000737:chemistry* / Q000494:pharmacology / Q000627:therapeutic use; D019073:HT29 Cells; D006801:Humans; D007700:Kinetics; D053758:Nanoparticles / Q000737:chemistry*; D010316:Particle Size; D011091:Polyesters / Q000737:chemistry*; D011100:Polyglycolic Acid / Q000737:chemistry*; D013499:Surface Properties
chemical_list: D011091:Polyesters; D011100:Polyglycolic Acid; C033616:poly(lactide); D048271:Chitosan; D005472:Fluorouracil
keywords: 5-Fluorouracil; Cytotoxicity; Nanoparticles