title: Isolation of a facultative anaerobic exoelectrogenic strain LZ-1 and probing electron transfer mechanism in situ by linking UV/Vis spectroscopy and electrochemistry.
abstract: A new facultative anaerobic exoelectrogenic strain LZ-1, belonging to Citrobacter freundii, has been isolated. This strain can produce current densities of 843.9 and 865.6μAcm-2using citrate or acetate as carbon source in a three-electrode configuration. The electricity generation performance was also analyzed in a dual-chamber MFC system, reaching a maximum power density of 1233mWm-2. In addition to acetate and citrate, other carbon sources such as pyruvate, formate, acetate, citrate and fumarate could also be utilized to produce current by strain LZ-1. Data supports the presence of electroactive c-type cytochromes in C. freundii sp. when grown on ITO electrodes, by linking spectroscopy and electrochemistry in situ. Since facultative strains possess many desirable properties compared to anaerobic strains, strain LZ-1 represents a promising exoelectrogenic species in engineering of biological catalysts for microbial electrochemistry.
authors.affiliation: College of Life Sciences, Northwest A&F University, No. 22 Xinong Road, Yangling, Shaanxi Province 712100, PR China.
authors.forename: Lei
authors.initials: L
authors.lastname: Zhou
authors.affiliation: College of Life Sciences, Northwest A&F University, No. 22 Xinong Road, Yangling, Shaanxi Province 712100, PR China.
authors.forename: Dandan
authors.initials: D
authors.lastname: Deng
authors.affiliation: College of Life Sciences, Northwest A&F University, No. 22 Xinong Road, Yangling, Shaanxi Province 712100, PR China.
authors.forename: Yichi
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: College of Life Sciences, Northwest A&F University, No. 22 Xinong Road, Yangling, Shaanxi Province 712100, PR China.
authors.forename: Wen
authors.initials: W
authors.lastname: Zhou
authors.affiliation: College of Life Sciences, Northwest A&F University, No. 22 Xinong Road, Yangling, Shaanxi Province 712100, PR China.
authors.forename: Yujing
authors.initials: Y
authors.lastname: Jiang
authors.affiliation: College of Life Sciences, Northwest A&F University, No. 22 Xinong Road, Yangling, Shaanxi Province 712100, PR China. Electronic address: lydiayliu@yahoo.com.
authors.forename: Ying
authors.initials: Y
authors.lastname: Liu
mesh_terms: D000085:Acetates / Q000378:metabolism; D001426:Bacterial Proteins / Q000378:metabolism; D001674:Bioelectric Energy Sources / Q000382:microbiology*; D002951:Citrates / Q000378:metabolism; D016971:Citrobacter freundii / Q000302:isolation & purification* / Q000378:metabolism*; D045304:Cytochromes c / Q000378:metabolism; D055664:Electrochemical Techniques; D004579:Electron Transport; D013056:Spectrophotometry, Ultraviolet
chemical_list: D000085:Acetates; D001426:Bacterial Proteins; D002951:Citrates; D045304:Cytochromes c
keywords: Citrobacter freundii; Facultative anaerobic strain; Microbial fuel cells; Spectroscopy and Electrochemistry; c-type cytochrome