title: Embryonic Explant Culture: Studying Effects of Regulatory Molecules on Gene Expression in Craniofacial Tissues.
abstract: The ex vivo culture of embryonic tissue explants permits the continuous monitoring of growth and morphogenesis at specific embryonic stages. The functions of soluble regulatory molecules can be analyzed by introducing them into culture medium or locally with beads to the tissue. Gene expression in the manipulated tissue explants can be analyzed using in situ hybridization, quantitative PCR, and reporter constructs combined to organ culture to examine the functions of the signaling molecules.
authors.affiliation: Institute for Molecular Medicine Finland, University of Helsinki, Biomedicum 2U, 20, Helsinki, 00014, Finland. katja.narhi@helsinki.fi.
authors.forename: Katja
authors.initials: K
authors.lastname: Närhi
mesh_terms: D000818:Animals; D047108:Embryonic Development / Q000235:genetics*; D005147:Facial Bones / Q000196:embryology*; D018507:Gene Expression Regulation, Developmental; D017403:In Situ Hybridization; D051379:Mice; D009024:Morphogenesis / Q000235:genetics*; D009924:Organ Culture Techniques; D038081:Organogenesis / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D012886:Skull / Q000196:embryology*
chemical_list: D012333:RNA, Messenger
keywords: Calvarial bone; In situ hybridization; Morphogenesis; Mouse; Organ culture; Palate; Real-time quantitative PCR; Signaling molecule; Tooth; Whisker