title: Comparison of potential protection conferred by three immunization strategies (protein/protein, DNA/DNA, and DNA/protein) against Brucella infection using Omp2b in BALB/c Mice.
abstract: In the present study, immunogenicity and protective efficacy of the Brucella outer membrane protein 2b (Omp2b) was evaluated in BALB/c mice using Protein/Protein, DNA/DNA and DNA/Protein vaccine strategies. Immunization of mice with three vaccine regimens elicited a strong specific IgG response (higher IgG2a titers over IgG1 titers) and provided Th1-oriented immune response. Vaccination of BALB/c mice with the DNA/Pro regimen induced higher levels of IFN-γ/IL-2 and conferred more protection levels against B. melitenisis and B. abortus challenge than did the protein or DNA alone. In conclusion, Omp2b is able to stimulate specific immune responses and to confer cross protection against B. melitensis and B. abortus infection. Therefore, it could be introduced as a new potential candidate for the development of a subunit vaccine against Brucella infection.
authors.affiliation: Department of Molecular Biology, Pasteur Institute of Iran, Tehran, Iran.
authors.forename: Maryam
authors.initials: M
authors.lastname: Golshani
authors.affiliation: Department of Immunotherapy and Leishmania Vaccine Research, Pasteur Institute of Iran, Tehran, Iran.
authors.forename: Sima
authors.initials: S
authors.lastname: Rafati
authors.affiliation: Bacterial Vaccine and Antigen Production Branch, Pasteur Institute of Iran, Karaj, Iran.
authors.forename: Mehdi
authors.initials: M
authors.lastname: Nejati-Moheimani
authors.affiliation: Department of Molecular Biology, Pasteur Institute of Iran, Tehran, Iran.
authors.forename: Melina
authors.initials: M
authors.lastname: Ghasemian
authors.affiliation: Department of Molecular Biology, Pasteur Institute of Iran, Tehran, Iran. Electronic address: saeidbouzari@yahoo.com.
authors.forename: Saeid
authors.initials: S
authors.lastname: Bouzari
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000276:immunology*; D002002:Brucella / Q000145:classification / Q000276:immunology; D002004:Brucella Vaccine / Q000276:immunology*; D002006:Brucellosis / Q000517:prevention & control*; D004269:DNA, Bacterial / Q000276:immunology*; D005260:Female; D051379:Mice; D008807:Mice, Inbred BALB C; D018272:Porins / Q000276:immunology*; D018417:Th1 Cells / Q000502:physiology; D014614:Vaccines, Synthetic / Q000276:immunology
chemical_list: D001426:Bacterial Proteins; D002004:Brucella Vaccine; D004269:DNA, Bacterial; C096736:Omp2b protein, Brucella; D018272:Porins; D014614:Vaccines, Synthetic
keywords: Brucella abortus; Brucella melitensis; Cross protection; DNA vaccine; Omp2b; Prime-boost strategy; Recombinant protein