title: Quantitative determination and evaluation of Paris polyphylla var. yunnanensis with different harvesting times using UPLC-UV-MS and FT-IR spectroscopy in combination with partial least squares discriminant analysis.
abstract: A rapid method was developed and validated by ultra-performance liquid chromatography-triple quadrupole mass spectroscopy with ultraviolet detection (UPLC-UV-MS) for simultaneous determination of paris saponin I, paris saponin II, paris saponin VI and paris saponin VII. Partial least squares discriminant analysis (PLS-DA) based on UPLC and Fourier transform infrared (FT-IR) spectroscopy was employed to evaluate Paris polyphylla var. yunnanensis (PPY) at different harvesting times. Quantitative determination implied that the various contents of bioactive compounds with different harvesting times may lead to different pharmacological effects; the average content of total saponins for PPY harvested at 8 years was higher than that from other samples. The PLS-DA of FT-IR spectra had a better performance than that of UPLC for discrimination of PPY from different harvesting times.
authors.affiliation: College of Traditional Chinese Medicine, Yunnan University of Traditional Chinese Medicine, Kunming, China.|Institute of Medicinal Plants, Yunnan Academy of Agricultural Sciences, Kunming, China.|Yunnan Technical Center for Quality of Chinese Materia Medica, Kunming, China.
authors.forename: Yuan-Gui
authors.initials: YG
authors.lastname: Yang
authors.affiliation: Institute of Medicinal Plants, Yunnan Academy of Agricultural Sciences, Kunming, China.|Yunnan Technical Center for Quality of Chinese Materia Medica, Kunming, China.
authors.forename: Ji
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Institute of Medicinal Plants, Yunnan Academy of Agricultural Sciences, Kunming, China.|Yunnan Technical Center for Quality of Chinese Materia Medica, Kunming, China.
authors.forename: Yan-Li
authors.initials: YL
authors.lastname: Zhao
authors.affiliation: Institute of Medicinal Plants, Yunnan Academy of Agricultural Sciences, Kunming, China.|Yunnan Technical Center for Quality of Chinese Materia Medica, Kunming, China.
authors.forename: Jin-Yu
authors.initials: JY
authors.lastname: Zhang
authors.affiliation: Institute of Medicinal Plants, Yunnan Academy of Agricultural Sciences, Kunming, China.|Yunnan Technical Center for Quality of Chinese Materia Medica, Kunming, China.
authors.forename: Yuan-Zhong
authors.initials: YZ
authors.lastname: Wang
mesh_terms: D002853:Chromatography, Liquid / Q000379:methods*; D016018:Least-Squares Analysis; D000070396:Liliales / Q000737:chemistry*; D013058:Mass Spectrometry / Q000379:methods*; D012015:Reference Standards; D013056:Spectrophotometry, Ultraviolet / Q000379:methods*; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods*
keywords: FT-IR; Paris polyphylla var. yunnanensis; UPLC-UV-MS; harvesting time; partial least squares discriminant analysis