title: Evaluation of antioxidant and hepatoprotective effects of white cabbage essential oil.
abstract: BACKGROUND
  There have been no reports of the extraction of essential oil (EO) from white cabbage [Brassica oleracea L. var. capitata (L.) Alef. f. alba DC. (Brassicaceae)] (Bocfal) or its chemical composition, antioxidant activity, or hepatoprotective effects.
  
  
  OBJECTIVE
  To extract Bocfal EO, to identify and quantify its chemical components, to assess their antioxidant capacity, and to evaluate the hepatoprotective properties of Bocfal EO.
  
  
  METHODS
  Bocfal EO was obtained using hydrodistillation (200 mm Hg/58 °C). The chemical composition was analyzed using GC-MS and was quantified using GC-FID. The antioxidant activity of Bocfal EO and its main constituents was evaluated using TBARS in rat brain homogenates. A Bocfal EO hepatoprotective effect (192 mg/kg) on acute carbon tetrachloride (CT)-induced liver damage was determined in rats using biochemical markers and histological analysis. Diallyl disulphide (DADS) (1 mmol/kg) was used as a control for comparison.
  
  
  RESULTS
  Bocfal EO contained organic polysulphides (OPSs), such as dimethyl trisulphide (DMTS) 65.43 ± 4.92% and dimethyl disulphide (DMDS) 19.29 ± 2.16% as major constituents. Bocfal EO and DMTS were found to be potent TBARS inhibitors with IC50values of 0.51 and 3 mg/L, respectively. Bocfal EO demonstrated better hepatoprotective properties than did DADS (p < 0.05), although both slightly affected the hepatic parenchyma per se, as observed using histopathology.
  
  
  CONCLUSIONS
  The antioxidant properties of Bocfal EO and DMTS may be the mechanism of hepatoprotective action; the parenchymal disturbances by Bocfal EO or DADS alone may be related to the high doses used.
authors.affiliation: a Escuela Nacional de Medicina y Homeopatía , Programa Institucional de Biomedicina Molecular, Instituto Politécnico Nacional , México.
authors.forename: Javier
authors.initials: J
authors.lastname: Morales-López
authors.affiliation: b Centro de Investigación en Ciencia Aplicada y Tecnología Avanzada del Instituto Politécnico Nacional , México.
authors.forename: Mónica
authors.initials: M
authors.lastname: Centeno-Álvarez
authors.affiliation: c Instituto de Química Universidad Nacional Autónoma de México , México.
authors.forename: Antonio
authors.initials: A
authors.lastname: Nieto-Camacho
authors.affiliation: d Unidad Irapuato , Centro de Investigación y de Estudios Avanzados del IPN , México.
authors.forename: Mercedes G
authors.initials: MG
authors.lastname: López
authors.affiliation: e IMSS , Hospital de Ortopedia "Dr. Victorio de la Fuente Narváez" , México.
authors.forename: Elizabeth
authors.initials: E
authors.lastname: Pérez-Hernández
authors.affiliation: a Escuela Nacional de Medicina y Homeopatía , Programa Institucional de Biomedicina Molecular, Instituto Politécnico Nacional , México.
authors.forename: Nury
authors.initials: N
authors.lastname: Pérez-Hernández
authors.affiliation: f Laboratory of Medicinal Chemistry and Pharmacology. Centro de Investigación en Biología de la Reproducción , Área Académica de Medicina, Universidad Autónoma del Estado de Hidalgo , Pachuca Hidalgo , México.
authors.forename: Eduardo
authors.initials: E
authors.lastname: Fernández-Martínez
mesh_terms: D000498:Allyl Compounds / Q000494:pharmacology; D000818:Animals; D000975:Antioxidants / Q000302:isolation & purification / Q000494:pharmacology*; D001921:Brain / Q000187:drug effects* / Q000378:metabolism; D001937:Brassica / Q000737:chemistry*; D002251:Carbon Tetrachloride; D056486:Chemical and Drug Induced Liver Injury / Q000209:etiology / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control*; D004195:Disease Models, Animal; D004220:Disulfides / Q000494:pharmacology; D008401:Gas Chromatography-Mass Spectrometry; D015227:Lipid Peroxidation / Q000187:drug effects; D008099:Liver / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D008297:Male; D009822:Oils, Volatile / Q000302:isolation & purification / Q000494:pharmacology*; D018384:Oxidative Stress / Q000187:drug effects*; D008517:Phytotherapy; D035261:Plant Components, Aerial; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology*; D010938:Plant Oils / Q000302:isolation & purification / Q000494:pharmacology*; D010946:Plants, Medicinal; D017208:Rats, Wistar; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
chemical_list: D000498:Allyl Compounds; D000975:Antioxidants; D004220:Disulfides; D009822:Oils, Volatile; D010936:Plant Extracts; D010938:Plant Oils; D017392:Thiobarbituric Acid Reactive Substances; C028009:diallyl disulfide; D002251:Carbon Tetrachloride
keywords: Brassica oleracea var. capitata f. alba; hepatoprotection; organic polysulphides