title: The diffuse reduction in spleen density: an indicator of severe acute pancreatitis?
abstract: We observed that acute pancreatitis (AP) was associated with diffuse reduction in spleen density (DROSD) in some patients. Furthermore, the condition of these patients was more serious, and the potential relationship between DROSD and structural and functional injury of the spleen remained unclear. Therefore, we performed a preliminary exploration of these factors. We analysed pertinent clinical data for AP patients with normal spleen density (control group) and for those with DROSD (reduction group) at the First Affiliated Hospital of Harbin Medical University (June 2013-June 2015). We measured the immunoglobulin M (IgM) B-cells of the AP patients and examined pancreatic and splenic tissues from AP rats with optical microscopy and TEM. The reduction group had a higher acute physiology and chronic health evaluation II (APACHE II) score, a longer length of stay (LOS) and lower serum calcium than the control group. The levels of triglycerides (TG) and total cholesterol (TC) did not differ significantly between the two groups. The percentage of IgM memory B-cells was significantly lower in the DROSD group than in the control group. TEM revealed that the spleen T-lymphocytes were normal in AP rats, but pyroptotic and necrotic spleen B-cells were observed in the severe AP rats. In AP, DROSD was an independent indicator of more severe conditions. Furthermore, spleen B-lymphocytes showed obvious damage at the cellular level, and the immunological function of the spleen was down-regulated when AP was associated with DROSD.
authors.affiliation: Department of Pancreatic and Biliary Surgery, First Affiliated Hospital of Harbin Medical University, 23 Youzheng Street, Nangang District, Harbin 150001, Heilongjiang Province, China.
authors.forename: Guangdong
authors.initials: G
authors.lastname: Shao
authors.affiliation: Department of Anesthesiology, First Affiliated Hospital of Harbin Medical University, 23 Youzheng Street, Nangang District, Harbin 150001, Heilongjiang Province, China.
authors.forename: Yanmei
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: Department of Hepatobiliary and Pancreatic Surgery, Third Affiliated Hospital of Harbin Medical University, 150 Haping Street, Nangang District, Harbin 150001, Heilongjiang Province, China.
authors.forename: Zengfu
authors.initials: Z
authors.lastname: Song
authors.affiliation: Department of Abdominal Ultrasonography, First Affiliated Hospital of Harbin Medical University, 23 Youzheng Street, Nangang District, Harbin 150001, Heilongjiang Province, China.
authors.forename: Maitao
authors.initials: M
authors.lastname: Jiang
authors.affiliation: Department of Pancreatic and Biliary Surgery, First Affiliated Hospital of Harbin Medical University, 23 Youzheng Street, Nangang District, Harbin 150001, Heilongjiang Province, China.
authors.forename: Xiaoqian
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Pancreatic and Biliary Surgery, First Affiliated Hospital of Harbin Medical University, 23 Youzheng Street, Nangang District, Harbin 150001, Heilongjiang Province, China.
authors.forename: Xiangren
authors.initials: X
authors.lastname: Jin
authors.affiliation: Department of Pancreatic and Biliary Surgery, First Affiliated Hospital of Harbin Medical University, 23 Youzheng Street, Nangang District, Harbin 150001, Heilongjiang Province, China.
authors.forename: Bei
authors.initials: B
authors.lastname: Sun
authors.affiliation: Department of Pancreatic and Biliary Surgery, First Affiliated Hospital of Harbin Medical University, 23 Youzheng Street, Nangang District, Harbin 150001, Heilongjiang Province, China baixuewei78@outlook.com.
authors.forename: Xuewei
authors.initials: X
authors.lastname: Bai
mesh_terms: D018806:APACHE; D000208:Acute Disease; D000818:Animals; D001402:B-Lymphocytes / Q000473:pathology; D006801:Humans; D007075:Immunoglobulin M / Q000032:analysis; D008055:Lipids / Q000097:blood; D008297:Male; D009929:Organ Size; D010195:Pancreatitis / Q000097:blood / Q000175:diagnosis / Q000473:pathology*; D017208:Rats, Wistar; D013154:Spleen / Q000473:pathology*
chemical_list: D007075:Immunoglobulin M; D008055:Lipids
keywords: B cell; acute pancreatitis; pyroptosis; spleen density; tuftsin