title: Genetic Diversity and Symbiotic Efficiency of Nodulating Rhizobia Isolated from Root Nodules of Faba Bean in One Field.
abstract: Thirty-one nodulating rhizobium strains were collected from root nodules of spring and winter type faba bean cultivars grown in micro ecoarea, i.e. the same field in Chengdu plain, China. The symbiotic efficiency and phylogeny of these strains were studied. Effectively nitrogen fixing strains were isolated from both winter type and spring type cultivars. Based on phylogenetic analysis of 16S rRNA gene and concatenated sequence of atpD, glnII and recA genes, the isolates were assigned as Rhizobium anhuiense and a potential new Rhizobium species. The isolates were diverse on symbiosis related gene level, carrying five, four and three variants of nifH, nodC and nodD, respectively. Strains carrying similar gene combinations were trapped by both winter and spring cultivars, disagreeing with the specificity of symbiotic genotypes to reported earlier faba bean ecotypes.
authors.affiliation: Department of Microbiology, College of Resources, Sichuan Agricultural University, Chengdu, China.
authors.forename: Lan
authors.initials: L
authors.lastname: Zou
authors.affiliation: Department of Microbiology, College of Resources, Sichuan Agricultural University, Chengdu, China.
authors.forename: Yuan Xue
authors.initials: YX
authors.lastname: Chen
authors.affiliation: Department of Environmental Sciences, University of Helsinki, Helsinki, Finland.
authors.forename: Petri
authors.initials: P
authors.lastname: Penttinen
authors.affiliation: Department of Microbiology, College of Resources, Sichuan Agricultural University, Chengdu, China.
authors.forename: Qin
authors.initials: Q
authors.lastname: Lan
authors.affiliation: Department of Microbiology, College of Resources, Sichuan Agricultural University, Chengdu, China.
authors.forename: Ke
authors.initials: K
authors.lastname: Wang
authors.affiliation: Department of Microbiology, College of Resources, Sichuan Agricultural University, Chengdu, China.
authors.forename: Ming
authors.initials: M
authors.lastname: Liu
authors.affiliation: Department of Microbiology, College of Resources, Sichuan Agricultural University, Chengdu, China.
authors.forename: Dan
authors.initials: D
authors.lastname: Peng
authors.affiliation: Department of Microbiology, College of Resources, Sichuan Agricultural University, Chengdu, China.
authors.forename: Xiaoping
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Department of Microbiology, College of Resources, Sichuan Agricultural University, Chengdu, China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Chen
authors.affiliation: Department of Microbiology, College of Resources, Sichuan Agricultural University, Chengdu, China.
authors.forename: Ke
authors.initials: K
authors.lastname: Zhao
authors.affiliation: Soil and Fertilizer Institute, Sichuan Academy of Agricultural Sciences, Chengdu, China.
authors.forename: Xiangzhong
authors.initials: X
authors.lastname: Zeng
authors.affiliation: Department of Microbiology, College of Resources, Sichuan Agricultural University, Chengdu, China.
authors.forename: Kai Wei
authors.initials: KW
authors.lastname: Xu
mesh_terms: D004269:DNA, Bacterial / Q000235:genetics; D005798:Genes, Bacterial; D014644:Genetic Variation; D010802:Phylogeny; D055170:Plant Root Nodulation; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012231:Rhizobium / Q000235:genetics* / Q000302:isolation & purification / Q000502:physiology; D053204:Root Nodules, Plant / Q000382:microbiology* / Q000502:physiology; D013559:Symbiosis; D031307:Vicia faba / Q000382:microbiology* / Q000502:physiology
chemical_list: D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S