title: Effects of green tea and bisphosphonate association on dental socket repair of rats.
abstract: OBJECTIVE
  To evaluate the effects of green tea intake and zoledronic acid intravenous therapy on teeth socket repair.
  
  
  METHODS
  Sixty male albinus Wistar rats were divided into 4 groups: C-Control, intravenous (IV) 0.9% saline solution (SS), GT-1% green tea in drinking water and IV SS, BP-IV zoledronic acid (BP), and BP+GT-IV BP and 1% green tea. 0.035mg/kg of BP was administered every two weeks. After ten weeks, right upper molars were extracted and the green tea started to be offered for GT and BP+GT. After 7, 14, and 28days the animals were euthanized.
  
  
  RESULTS
  Histopathology analysis revealed lack of socket repair in BP and BP+GT groups, which presented significant increased number of polimorphonuclear leukocytes at day 28, in comparison with C (p<0.05). No significant differences were detected between C and the experimental groups at the same period (p<0.05) when considering mononuclear leukocytes. Immunolabeling revealed that the association of BP and GT caused a slight disturbance in OPG/RANKL system and retarded Runx-2 labeling. Although strong TRAP labeling was observed, most of the positive cells in BP and BP+GT groups were not located on bone surface.
  
  
  CONCLUSIONS
  Socket healing of rats treated with BP and regular drinking green tea presented no relevant differences in comparison to those treated with BP alone.
authors.affiliation: Department of Health Sciences, Sagrado Coração Univesity - USC, Rua Irmã Arminda 10-50, 17011-160, Bauru, SP, Brazil.
authors.forename: Edson Yoshihiro
authors.initials: EY
authors.lastname: Mada
authors.affiliation: Department of Health Sciences, Sagrado Coração Univesity - USC, Rua Irmã Arminda 10-50, 17011-160, Bauru, SP, Brazil.
authors.forename: Alana Claro Cunha
authors.initials: AC
authors.lastname: Santos
authors.affiliation: Department of Biological Science, São Paulo University - FOB/USP, Alameda Otávio Pinheiro Brisola, 9-75, Bauru, SP, 17012-901, Brazil.
authors.forename: Angelica Cristina
authors.initials: AC
authors.lastname: Fonseca
authors.affiliation: Department of Biological Science, São Paulo University - FOB/USP, Alameda Otávio Pinheiro Brisola, 9-75, Bauru, SP, 17012-901, Brazil.
authors.forename: Claudia Cristina
authors.initials: CC
authors.lastname: Biguetti
authors.affiliation: Department of Health Sciences, Sagrado Coração Univesity - USC, Rua Irmã Arminda 10-50, 17011-160, Bauru, SP, Brazil.
authors.forename: Fernando Tozze Alves
authors.initials: FT
authors.lastname: Neves
authors.affiliation: Department of Health Sciences, Sagrado Coração Univesity - USC, Rua Irmã Arminda 10-50, 17011-160, Bauru, SP, Brazil.
authors.forename: Patrícia Pinto
authors.initials: PP
authors.lastname: Saraiva
authors.affiliation: Department of Health Sciences, Sagrado Coração Univesity - USC, Rua Irmã Arminda 10-50, 17011-160, Bauru, SP, Brazil; Department of Basic Sciences, São Paulo State University (Unesp), School of Dentistry, Araçatuba, Rua José Bonifácio 1193, 16015-050, Araçatuba, SP, Brazil. Electronic address: mariza.matsumoto@foa.unesp.br.
authors.forename: Mariza Akemi
authors.initials: MA
authors.lastname: Matsumoto
mesh_terms: D000818:Animals; D050659:Core Binding Factor Alpha 1 Subunit / Q000032:analysis; D004164:Diphosphonates / Q000494:pharmacology*; D004338:Drug Combinations; D007093:Imidazoles / Q000494:pharmacology*; D007150:Immunohistochemistry; D007962:Leukocytes; D008297:Male; D008437:Maxilla; D010020:Osteonecrosis / Q000188:drug therapy; D053244:Osteoprotegerin / Q000032:analysis; D010936:Plant Extracts / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D000071681:Tartrate-Resistant Acid Phosphatase / Q000032:analysis; D013662:Tea / Q000737:chemistry*; D020390:Tooth Socket / Q000187:drug effects* / Q000473:pathology; D014945:Wound Healing / Q000187:drug effects*; D000077211:Zoledronic Acid
chemical_list: D050659:Core Binding Factor Alpha 1 Subunit; D004164:Diphosphonates; D004338:Drug Combinations; D007093:Imidazoles; D053244:Osteoprotegerin; D010936:Plant Extracts; C493717:Runx2 protein, rat; D013662:Tea; C506032:Tnfrsf11b protein, rat; D000077211:Zoledronic Acid; D000071681:Tartrate-Resistant Acid Phosphatase
keywords: Bisphosphonates; Bone repair; Green tea; Immunohistochemistry; Osteonecrosis; Rats