title: Engineering anatase hierarchically cactus-like TiO2arrays for photoelectrochemical and visualized sensing platform.
abstract: This work described that one-step synthesis three dimensional anatase hierarchically cactus-like TiO2arrays (AHCT) and their application in constructing a novel photoelectrochemical (PEC) and visualized sensing platform based on molecular imprinting technique, which reports its result with the prussian blue (PB) electrode served as the electrochromic indicator for the detection of glycoprotein (RNase B). The AHCT arrays were perpendicularly grown on FTO substrate with tunable sizes, offering many advantages, such as large contact area, rapid charge electron separation and transport. A possible formation process of the interesting AHCT arrays has been investigated based on time-dependent experiment. In addition, the PEC and visualized sensing platform was constructed based on the molecularly imprinted polymer modified AHCT arrays. Specifically, in the proposed system, the more RNase B being, the more insulating layer was formed on the surface of AHCT arrays that impeded the harvesting of light and electron transfer, resulting in the reduction of photocurrent. When upon light illumination, the photogenerated electrons flow through an external circuit to PB, leading to the reduction of PB to prussian white (PW), which is transparent. The rate of decolourization of PB is proportional to the concentration of RNase B. In this way, a visualized PEC sensing platform that gives its quantitative information could be performed by monitoring the change of color intensity. Under optimal conditions, the protocol possessed a detection range of 0.5pM to 2μM (r=0.997) and the limit of detection was 0.12 pM toward RNase B. Our method eliminates the need for sophisticated instruments and high detection expenses, making it possible to be a reliable alternative in resource-constrained regions.
authors.affiliation: Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, University of Jinan, Jinan 250022, PR China.
authors.forename: Chaomin
authors.initials: C
authors.lastname: Gao
authors.affiliation: Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, University of Jinan, Jinan 250022, PR China.
authors.forename: Yanhu
authors.initials: Y
authors.lastname: Wang
authors.affiliation: School of Material Science and Engineering, Materials Research Center for Energy and Photoelectrochemical Conversion, University of Jinan, Jinan 250022, PR China.
authors.forename: Shuai
authors.initials: S
authors.lastname: Yuan
authors.affiliation: Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, University of Jinan, Jinan 250022, PR China.
authors.forename: Jie
authors.initials: J
authors.lastname: Xue
authors.affiliation: School of Material Science and Engineering, Materials Research Center for Energy and Photoelectrochemical Conversion, University of Jinan, Jinan 250022, PR China.
authors.forename: Bingqiang
authors.initials: B
authors.lastname: Cao
authors.affiliation: Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, University of Jinan, Jinan 250022, PR China. Electronic address: ujn.yujh@gmail.com.
authors.forename: Jinghua
authors.initials: J
authors.lastname: Yu
mesh_terms: D015374:Biosensing Techniques; D055664:Electrochemical Techniques; D006023:Glycoproteins / Q000737:chemistry / Q000302:isolation & purification*; D054802:Molecular Imprinting; D011108:Polymers / Q000737:chemistry; D012260:Ribonucleases / Q000737:chemistry / Q000302:isolation & purification*; D014025:Titanium / Q000737:chemistry*
chemical_list: D006023:Glycoproteins; D011108:Polymers; C009495:titanium dioxide; D014025:Titanium; D012260:Ribonucleases; C029600:ribonuclease B
keywords: Anatase hierarchically cactus–like TiO(2); Electrochromic; Molecular imprinting technique; Photoelectrochemical; Prussian blue