title: Niosomes Consisting of Tween-60 and Cholesterol Improve the Chemical Stability and Antioxidant Activity of (-)-Epigallocatechin Gallate under Intestinal Tract Conditions.
abstract: In order to improve the chemical stability and antioxidant activity of (-)-epigallocatechin gallate (EGCG) in the gastrointestinal tract, niosomes composed of Tween-60 and cholesterol were developed to encapsulate EGCG in this investigation. EGCG loaded niosomes with encapsulation efficiency around 76% exhibited a small Z-average diameter about 60 nm. Compared to free EGCG, the EGCG remaining in dialysis tubes was significantly improved for niosomes at pH 2 and 7.4. Meanwhile, the residual EGCG for niosomes increased from 3% to 49% after 2 h incubation in simulated intestinal fluid (SIF). Pancreatin was found to impact the stability of niosomes in SIF mainly. Furthermore, the results from ferric reducing antioxidant power and cellular antioxidant activity tests indicated that EGCG loaded niosomes exhibited stronger antioxidant ability than free EGCG during intestinal digestion. Thus, we can infer that niosomal encapsulation might be a promising approach to improve the oral bioavailability of EGCG in the body.
authors.affiliation: Key Laboratory of Food Colloids and Biotechnology, Ministry of Education, School of Chemical and Material Engineering, Jiangnan University , Wuxi 214122, P. R. China.
authors.forename: Rong
authors.initials: R
authors.lastname: Liang
authors.affiliation: Key Laboratory of Food Colloids and Biotechnology, Ministry of Education, School of Food Science and Technology, Jiangnan University , Wuxi 214122, P. R. China.
authors.forename: Ling
authors.initials: L
authors.lastname: Chen
authors.affiliation: Western Regional Research Center, ARS, USDA , Albany, California 94710, United States.
authors.forename: Wallace
authors.initials: W
authors.lastname: Yokoyama
authors.affiliation: Centre for Water-Soluble Polymers, North East Wales Institute , Plas Coch, Mold Road, Wrexham LL11 2AW, U.K.
authors.forename: Peter A
authors.initials: PA
authors.lastname: Williams
authors.affiliation: Key Laboratory of Food Colloids and Biotechnology, Ministry of Education, School of Food Science and Technology, Jiangnan University , Wuxi 214122, P. R. China.
authors.forename: Fang
authors.initials: F
authors.lastname: Zhong
mesh_terms: D000975:Antioxidants / Q000494:pharmacology*; D001682:Biological Availability; D002392:Catechin / Q000031:analogs & derivatives* / Q000494:pharmacology; D002784:Cholesterol / Q000737:chemistry; D004063:Digestion; D004337:Drug Carriers / Q000737:chemistry*; D004355:Drug Stability; D056945:Hep G2 Cells; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007413:Intestinal Mucosa / Q000378:metabolism*; D008081:Liposomes / Q000737:chemistry*; D053758:Nanoparticles / Q000737:chemistry; D010194:Pancreatin / Q000737:chemistry; D010316:Particle Size; D011136:Polysorbates / Q000737:chemistry
chemical_list: D000975:Antioxidants; D004337:Drug Carriers; D008081:Liposomes; D011136:Polysorbates; D010194:Pancreatin; D002392:Catechin; D002784:Cholesterol; C045651:epigallocatechin gallate
keywords: (−)-epigallocatechin gallate (EGCG); cellular antioxidant activity (CAA); digestive stability; niosomes