title: Stable plastid transformation inScoparia dulcisL.
abstract: In the present investigation we report stable plastid transformation inScoparia dulcisL., a versatile medicinal herb via particle gun method. The vector KNTc, harbouringaadAas a selectable marker andegfpas a reporter gene which were under the control of synthetic promoter pNG1014a, targets inverted repeats,trnR/trnNof the plastid genome. By use of this heterologous vector, recovery of transplastomic lines with suitable selection protocol have been successfully established with overall efficiency of two transgenic lines for 25 bombarded leaf explants. PCR and Southern blot analysis demonstrated stable integration of foreign gene into the target sequences. The results represent a significant advancement of the plastid transformation technology in medicinal plants, which relevantly implements a change over in enhancing and regulating of certain metabolic pathways.
authors.affiliation: Department of Biotechnology, Kakatiya University, Warangal, 506009 India.
authors.forename: Narra
authors.initials: N
authors.lastname: Muralikrishna
authors.affiliation: Department of Biotechnology, Kakatiya University, Warangal, 506009 India.
authors.forename: Kota
authors.initials: K
authors.lastname: Srinivas
authors.affiliation: Department of Biotechnology, Kakatiya University, Warangal, 506009 India.
authors.forename: Kalva Bharath
authors.initials: KB
authors.lastname: Kumar
authors.affiliation: Department of Biotechnology, Kakatiya University, Warangal, 506009 India.
authors.forename: Abbagani
authors.initials: A
authors.lastname: Sadanandam
keywords: Particle bombardment; Plastid transformation; Scoparia dulcis L.; aadA; egfp