title: A Low-Cost High-Definition Video System for Microsurgical Hindlimb Replantation in Rats.
abstract: BackgroundThe surgical microscope is still essential for microsurgery, but several alternatives that show promising results are currently under development, such as endoscopes and laparoscopes with video systems; however, as yet, these have only been used for arterial anastomoses. The aim of this study was to evaluate the use of a low-cost video-assisted magnification system in replantation of the hindlimbs of rats.MethodsThirty Wistar rats were randomly divided into two matched groups according to the magnification system used: the microscope group, with hindlimb replantation performed under a microscope with an image magnification of 40× and the video group, with the procedures performed under a video system composed of a high-definition camcorder, macrolenses, a 42-in television, and a digital HDMI cable. The camera was set to 50× magnification. We analyzed weight, arterial and venous caliber, total surgery time, arterial and venous anastomosis time, patency immediately and 7 days postoperatively, the number of stitches, and survival rate.ResultsThere were no significant differences between the groups in weight, arterial or venous caliber, or the number of stitches. Replantation under the video system took longer (p< 0.05). Patency rates were similar between groups, both immediately and 7 days postoperatively.ConclusionIt is possible to perform a hindlimb replantation in rats through video system magnification, with a satisfactory success rate comparable with that for procedures performed under surgical microscopes.
authors.forename: Rui
authors.initials: R
authors.lastname: Sergio
authors.affiliation: Department of Orthopedic Surgery, State University of Para Belem, Para, Brazil.
authors.forename: Monteiro
authors.initials: M
authors.lastname: de Barros
authors.affiliation: Department of Experimental Surgery, State University of Para Belem, Para, Brazil.
authors.forename: Marcus Vinicius Henriques
authors.initials: MV
authors.lastname: Brito
authors.affiliation: Department of Orthopedic Surgery, State University of Para Belem, Para, Brazil.
authors.forename: Rafael Aquino
authors.initials: RA
authors.lastname: Leal
authors.affiliation: Department of Experimental Surgery, State University of Para Belem, Para, Brazil.
authors.forename: Renan Kleber Costa
authors.initials: RK
authors.lastname: Teixeira
authors.affiliation: Department of Experimental Surgery, State University of Para Belem, Para, Brazil.
authors.forename: Marcelo Ferreia
authors.initials: MF
authors.lastname: Sabbá
authors.affiliation: Department of Experimental Surgery, State University of Para Belem, Para, Brazil.
authors.forename: Vitor Nagai
authors.initials: VN
authors.lastname: Yamaki
authors.affiliation: Department of Orthopedic Surgery, State University of Para Belem, Para, Brazil.
authors.forename: Marcos Vinicius Vieira
authors.initials: MV
authors.lastname: Lemos
mesh_terms: D000714:Anastomosis, Surgical / Q000191:economics / Q000295:instrumentation; D000818:Animals; D003362:Cost-Benefit Analysis; D005260:Female; D006614:Hindlimb / Q000601:surgery*; D008866:Microsurgery / Q000191:economics; D023421:Models, Animal; D051381:Rats; D017208:Rats, Wistar; D012092:Replantation / Q000191:economics / Q000295:instrumentation; D014654:Vascular Patency; D014656:Vascular Surgical Procedures / Q000191:economics; D020535:Video-Assisted Surgery / Q000191:economics*