title: Unexpected regulation of miRNA abundance during adaptation of early-somite mouse embryos to diabetic pregnancy.
abstract: Maternal diabetes is one of major causes of congenital malformations in offspring, but the underlying mechanism is still unclear. MiRNAs play an important role in transcriptional and post-transcriptional regulation of gene expression. However, no miRNA expression profiling of hyperglycemic offspring are thus far available. Female mice were made diabetic with streptozotocin, treated with slow-release insulin tablets, and mated. MiRNA expression profiling with Next Generation Sequencing on the SOLiD5 platform was performed on 8 control and 5 hyperglycemic embryonic day (ED)8.5 and 9 control and 6 hyperglycemic ED9.5 embryos. Differential expression was analyzed with the Wald test. On ED8.5, the abundance of expressed miRNAs was similar in control and hyperglycemic ED8.5 embryos. The spectrum of expressed miRNAs had not changed in ED9.5 embryos, but the abundance of most miRNAs increased ∼5-fold in control embryos. However, hyperglycemic D9.5 embryos were unable to mount this increase in prevalence. Only 3 miRNAs were differentially expressed in control and hyperglycemic ED9.5 embryos, but their putative target genes were underrepresented in the Jackson database of genes causing cardiovascular or neural malformations.
authors.affiliation: Tytgat Institute for Liver and Intestinal Research, Academic Medical Center, University of Amsterdam, Meibergdreef 69-71, 1105BK, Amsterdam, The Netherlands.
authors.forename: J
authors.initials: J
authors.lastname: Zhao
authors.affiliation: Tytgat Institute for Liver and Intestinal Research, Academic Medical Center, University of Amsterdam, Meibergdreef 69-71, 1105BK, Amsterdam, The Netherlands.
authors.forename: T B M
authors.initials: TBM
authors.lastname: Hakvoort
authors.affiliation: Department of Bioinformatics, Academic Medical Center, University of Amsterdam, Meibergdreef 69-71, 1105BK, Amsterdam, The Netherlands.
authors.forename: A
authors.initials: A
authors.lastname: Jongejan
authors.affiliation: Department of Anatomy, Embryology & Physiology, Academic Medical Center, University of Amsterdam, Meibergdreef 69-71, 1105BK, Amsterdam, The Netherlands.
authors.forename: J M
authors.initials: JM
authors.lastname: Ruijter
authors.affiliation: Department of Bioinformatics, Academic Medical Center, University of Amsterdam, Meibergdreef 69-71, 1105BK, Amsterdam, The Netherlands.
authors.forename: A H C
authors.initials: AHC
authors.lastname: van Kampen
authors.affiliation: Tytgat Institute for Liver and Intestinal Research, Academic Medical Center, University of Amsterdam, Meibergdreef 69-71, 1105BK, Amsterdam, The Netherlands. Electronic address: w.h.lamers@amc.uva.nl.
authors.forename: W H
authors.initials: WH
authors.lastname: Lamers
mesh_terms: D000818:Animals; D004622:Embryo, Mammalian / Q000378:metabolism; D005260:Female; D018507:Gene Expression Regulation, Developmental; D006943:Hyperglycemia / Q000209:etiology / Q000235:genetics; D008297:Male; D051379:Mice; D035683:MicroRNAs / Q000235:genetics*; D011247:Pregnancy; D011254:Pregnancy in Diabetics / Q000209:etiology; D019170:Somites / Q000196:embryology* / Q000378:metabolism; D059467:Transcriptome
chemical_list: D035683:MicroRNAs
keywords: Maternal hyperglycemia; Perigastrulation stage; miRNA expression