title: Simultaneous stimulation of sedoheptulose 1,7-bisphosphatase, fructose 1,6-bisphophate aldolase and the photorespiratory glycine decarboxylase-H protein increases CO2assimilation, vegetative biomass and seed yield in Arabidopsis.
abstract: In this article, we have altered the levels of three different enzymes involved in the Calvin-Benson cycle and photorespiratory pathway. We have generated transgenic Arabidopsis plants with altered combinations of sedoheptulose 1,7-bisphosphatase (SBPase), fructose 1,6-bisphophate aldolase (FBPA) and the glycine decarboxylase-H protein (GDC-H) gene identified as targets to improve photosynthesis based on previous studies. Here, we show that increasing the levels of the three corresponding proteins, either independently or in combination, significantly increases the quantum efficiency of PSII. Furthermore, photosynthetic measurements demonstrated an increase in the maximum efficiency of CO2fixation in lines over-expressing SBPase and FBPA. Moreover, the co-expression of GDC-H with SBPase and FBPA resulted in a cumulative positive impact on leaf area and biomass. Finally, further analysis of transgenic lines revealed a cumulative increase of seed yield in SFH lines grown in high light. These results demonstrate the potential of multigene stacking for improving the productivity of food and energy crops.
authors.affiliation: School of Biological Sciences, University of Essex, Colchester, UK.
authors.forename: Andrew J
authors.initials: AJ
authors.lastname: Simkin
authors.affiliation: School of Biological Sciences, University of Essex, Colchester, UK.
authors.forename: Patricia E
authors.initials: PE
authors.lastname: Lopez-Calcagno
authors.affiliation: School of Biological Sciences, University of Essex, Colchester, UK.
authors.forename: Philip A
authors.initials: PA
authors.lastname: Davey
authors.affiliation: School of Biological Sciences, University of Essex, Colchester, UK.
authors.forename: Lauren R
authors.initials: LR
authors.lastname: Headland
authors.affiliation: School of Biological Sciences, University of Essex, Colchester, UK.
authors.forename: Tracy
authors.initials: T
authors.lastname: Lawson
authors.affiliation: Plant Physiology Department, University of Rostock, Rostock, Germany.
authors.forename: Stefan
authors.initials: S
authors.lastname: Timm
authors.affiliation: Plant Physiology Department, University of Rostock, Rostock, Germany.
authors.forename: Hermann
authors.initials: H
authors.lastname: Bauwe
authors.affiliation: School of Biological Sciences, University of Essex, Colchester, UK.
authors.forename: Christine A
authors.initials: CA
authors.lastname: Raines
mesh_terms: D017360:Arabidopsis / Q000254:growth & development* / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism; D018533:Biomass; D002245:Carbon Dioxide / Q000378:metabolism*; D005634:Fructose-Bisphosphate Aldolase / Q000235:genetics / Q000378:metabolism*; D050961:Glycine Decarboxylase Complex H-Protein / Q000235:genetics / Q000378:metabolism*; D008027:Light; D010744:Phosphoric Monoester Hydrolases / Q000235:genetics / Q000378:metabolism*; D010788:Photosynthesis / Q000235:genetics / Q000502:physiology; D030821:Plants, Genetically Modified / Q000235:genetics / Q000378:metabolism; D012639:Seeds / Q000254:growth & development* / Q000378:metabolism
chemical_list: D029681:Arabidopsis Proteins; D050961:Glycine Decarboxylase Complex H-Protein; D002245:Carbon Dioxide; D010744:Phosphoric Monoester Hydrolases; C022627:sedoheptulose-bisphosphatase; D005634:Fructose-Bisphosphate Aldolase
keywords: FBP aldolase; SBPase; chlorophyll fluorescence imaging; glycine decarboxylase-H protein; photosynthesis; transgenic