title: Ribosomal Peptide Syntheses from Activated Substrates Reveal Rate Limitation by an Unexpected Step at the Peptidyl Site.
abstract: Protein synthesis (translation) is central to cellular function and antibiotic development. Interestingly, the key chemical step of translation, peptide bond formation, is among the slower enzymatic reactions. The reason for this remains controversial because of reliance on studies using highly modified, severely minimized, or unreactive substrate analogues. Here, we investigated this problem by fast kinetics using full-length aminoacyl-tRNA substrates with atomic substitutions that activated the ester electrophile. While trifluoro substitution of hydrogens in nonconserved positions of the peptidyl-site substrate dramatically increased the ester reactivity in solution assays, a large hastening of the combined rates of ribosomal accommodation and peptidyl transfer was observed only with a slowly reacting aminoacyl-site nucleophile, proline. With a fast-reacting A-site nucleophile, phenylalanine, effects did not correlate at all with electrophilicities. As effects were observed using the same, natural, aminoacyl-tRNA at the A site and all rates of accommodation/peptidyl transfer were pH dependent, we concluded that rate limitation was not by A-site accommodation but rather by peptidyl transfer and a hitherto unexpected step at the P site. This new slow step, which we term P-site accommodation, has implications for the activation or inhibition of ribosome function in vitro and in vivo.
authors.affiliation: Department of Cell and Molecular Biology, Uppsala University , Husargatan 3, Box 596, Uppsala 75124, Sweden.
authors.forename: Jinfan
authors.initials: J
authors.lastname: Wang
authors.affiliation: Department of Cell and Molecular Biology, Uppsala University , Husargatan 3, Box 596, Uppsala 75124, Sweden.
authors.forename: Marek
authors.initials: M
authors.lastname: Kwiatkowski
authors.affiliation: Department of Cell and Molecular Biology, Uppsala University , Husargatan 3, Box 596, Uppsala 75124, Sweden.
authors.forename: Anthony C
authors.initials: AC
authors.lastname: Forster
mesh_terms: D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D015394:Molecular Structure; D010453:Peptide Synthases / Q000737:chemistry / Q000378:metabolism*; D010455:Peptides / Q000737:chemistry / Q000378:metabolism*; D014176:Protein Biosynthesis; D012343:RNA, Transfer / Q000737:chemistry / Q000378:metabolism*; D012270:Ribosomes / Q000201:enzymology*; D013379:Substrate Specificity
chemical_list: D010455:Peptides; D012343:RNA, Transfer; D010453:Peptide Synthases