title: Plasmodium knowlesi: a relevant, versatile experimental malaria model.
abstract: The primate malaria Plasmodium knowlesi has a long-standing history as an experimental malaria model. Studies using this model parasite in combination with its various natural and experimental non-human primate hosts have led to important advances in vaccine development and in our understanding of malaria invasion, immunology and parasite-host interactions. The adaptation to long-term in vitro continuous blood stage culture in rhesus monkey, Macaca fascicularis and human red blood cells, as well as the development of various transfection methodologies has resulted in a highly versatile experimental malaria model, further increasing the potential of what was already a very powerful model. The growing evidence that P. knowlesi is an important human zoonosis in South-East Asia has added relevance to former and future studies of this parasite species.
authors.affiliation: Department of Parasitology,Biomedical Primate Research Centre,PO Box 3306, 2280 GH Rijswijk,The Netherlands.
authors.forename: Erica M
authors.initials: EM
authors.lastname: Pasini
authors.affiliation: Department of Parasitology,Biomedical Primate Research Centre,PO Box 3306, 2280 GH Rijswijk,The Netherlands.
authors.forename: Anne-Marie
authors.initials: AM
authors.lastname: Zeeman
authors.affiliation: Department of Parasitology,Biomedical Primate Research Centre,PO Box 3306, 2280 GH Rijswijk,The Netherlands.
authors.forename: Annemarie
authors.initials: A
authors.lastname: Voorberg-VAN DER Wel
authors.affiliation: Department of Parasitology,Biomedical Primate Research Centre,PO Box 3306, 2280 GH Rijswijk,The Netherlands.
authors.forename: Clemens H M
authors.initials: CHM
authors.lastname: Kocken
mesh_terms: D000220:Adaptation, Biological; D000818:Animals; D004195:Disease Models, Animal; D004912:Erythrocytes / Q000469:parasitology; D000882:Haplorhini; D006790:Host-Parasite Interactions; D006801:Humans; D008252:Macaca fascicularis; D008253:Macaca mulatta; D008288:Malaria / Q000276:immunology / Q000469:parasitology* / Q000517:prevention & control / Q000662:veterinary; D017780:Malaria Vaccines / Q000032:analysis / Q000494:pharmacology; D008992:Monkey Diseases / Q000276:immunology / Q000469:parasitology / Q000517:prevention & control; D016790:Plasmodium knowlesi / Q000276:immunology / Q000502:physiology*; D015047:Zoonoses / Q000276:immunology / Q000469:parasitology / Q000517:prevention & control
chemical_list: D017780:Malaria Vaccines
keywords: 
                 Plasmodium knowlesi
              ; 
                 in vitro
              ; malaria; model; non-human primates; severe malaria; transfection; vaccine; zoonosis