title: Suppression of Cell Wall Degrading Enzymes and their Encoding Genes in Button Mushrooms (Agaricus bisporus) by CaCl2and Citric Acid.
abstract: Fresh button mushrooms (Agaricus bisporus) were harvested and treated with a solution of 1.5% CaCl2+ 0.5% citric acid and stored for 16 days at 12 °C. The effects of this treatment on firmness, weight, color, cell wall compositions (cellulose and chitin) and cell wall degrading enzymes (cel1ulase, beta-1, 3 glucanase, chitinase and phenylalanine ammonialyase) were investigated during post-harvest storage. The expressions of major genes (Cel1, Glu1, Chi1 and PAL1) involved in cell wall degradation during post-harvest storage were also monitored. The results revealed that the post-harvest chemical treatment maintained better firmness, weight, color and inhibited cellulase, beta-1, 3 glucanase, chitinase and phenylalanine ammonialyase activities. These findings showed that the down-regulation of cell wall degrading enzymes is a possible mechanism that delays the softening of button mushrooms by the application of combined chemical treatment.
authors.affiliation: College of Biosystems Engineering and Food Science, Fuli Institute of Food Science, Zhejiang Key Laboratory for Agro-Food Processing, Zhejiang R & D Center for Food Technology and Equipment, Zhejiang University, Hangzhou, 310058, People's Republic of China. ziakhujari@yahoo.com.|Department of Agriculture, Abdul Wali Khan University, Mardan, Khyber-Pakhtunkhwa, 23200, Pakistan. ziakhujari@yahoo.com.
authors.forename: Zia Ullah
authors.initials: ZU
authors.lastname: Khan
authors.affiliation: College of Biosystems Engineering and Food Science, Fuli Institute of Food Science, Zhejiang Key Laboratory for Agro-Food Processing, Zhejiang R & D Center for Food Technology and Equipment, Zhejiang University, Hangzhou, 310058, People's Republic of China.
authors.forename: Li
authors.initials: L
authors.lastname: Jiayin
authors.affiliation: Department of Chemistry, Shaheed Benazir Bhutto University, Sheringal, Dir (Upper), Khyber-Pakhtunkhwa, 18000, Pakistan.
authors.forename: Nasir Mehmood
authors.initials: NM
authors.lastname: Khan
authors.affiliation: College of Biosystems Engineering and Food Science, Fuli Institute of Food Science, Zhejiang Key Laboratory for Agro-Food Processing, Zhejiang R & D Center for Food Technology and Equipment, Zhejiang University, Hangzhou, 310058, People's Republic of China.
authors.forename: Wangshu
authors.initials: W
authors.lastname: Mou
authors.affiliation: College of Biosystems Engineering and Food Science, Fuli Institute of Food Science, Zhejiang Key Laboratory for Agro-Food Processing, Zhejiang R & D Center for Food Technology and Equipment, Zhejiang University, Hangzhou, 310058, People's Republic of China.
authors.forename: Dongdong
authors.initials: D
authors.lastname: Li
authors.affiliation: College of Biosystems Engineering and Food Science, Fuli Institute of Food Science, Zhejiang Key Laboratory for Agro-Food Processing, Zhejiang R & D Center for Food Technology and Equipment, Zhejiang University, Hangzhou, 310058, People's Republic of China.
authors.forename: Yansheng
authors.initials: Y
authors.lastname: Wang
authors.affiliation: College of Biosystems Engineering and Food Science, Fuli Institute of Food Science, Zhejiang Key Laboratory for Agro-Food Processing, Zhejiang R & D Center for Food Technology and Equipment, Zhejiang University, Hangzhou, 310058, People's Republic of China.
authors.forename: Simin
authors.initials: S
authors.lastname: Feng
authors.affiliation: College of Biosystems Engineering and Food Science, Fuli Institute of Food Science, Zhejiang Key Laboratory for Agro-Food Processing, Zhejiang R & D Center for Food Technology and Equipment, Zhejiang University, Hangzhou, 310058, People's Republic of China.
authors.forename: Zisheng
authors.initials: Z
authors.lastname: Luo
authors.affiliation: College of Biosystems Engineering and Food Science, Fuli Institute of Food Science, Zhejiang Key Laboratory for Agro-Food Processing, Zhejiang R & D Center for Food Technology and Equipment, Zhejiang University, Hangzhou, 310058, People's Republic of China.
authors.forename: Linchun
authors.initials: L
authors.lastname: Mao
authors.affiliation: College of Biosystems Engineering and Food Science, Fuli Institute of Food Science, Zhejiang Key Laboratory for Agro-Food Processing, Zhejiang R & D Center for Food Technology and Equipment, Zhejiang University, Hangzhou, 310058, People's Republic of China.
authors.forename: Tiejin
authors.initials: T
authors.lastname: Ying
mesh_terms: D000364:Agaricus / Q000187:drug effects* / Q000201:enzymology / Q000235:genetics; D002122:Calcium Chloride / Q000494:pharmacology*; D002473:Cell Wall / Q000187:drug effects / Q000201:enzymology; D019343:Citric Acid / Q000494:pharmacology*; D015536:Down-Regulation; D005519:Food Preservation / Q000379:methods*; D013997:Time Factors
chemical_list: D019343:Citric Acid; D002122:Calcium Chloride
keywords: Button mushroom; Cell wall degrading enzymes; Chemical treatment; Texture