title: Automated Enrichment of Sulfanilamide in Milk Matrices by Utilization of Aptamer-Linked Magnetic Particles.
abstract: The present work demonstrates the first automated enrichment approach for antibiotics in milk using specific DNA aptamers. First, aptamers toward the antibiotic sulfanilamide were selected and characterized regarding their dissociation constants and specificity toward relevant antibiotics via fluorescence assay and LC-MS/MS detection. The performed enrichment was automated using the KingFisherDuo and compared to a manual approach. Verifying the functionality, trapping was realized in different milk matrices: (i) 0.3% fat milk, (ii) 1.5% fat milk, (iii) 3.5% fat milk, and (iv) 0.3% fat cocoa milk drink. Enrichment factors up to 8-fold could be achieved. Furthermore, it could be shown that novel implementation of a magnetic separator increases the reproducibility and reduces the hands-on time from approximately half a day to 30 min.
authors.affiliation: Hamburg School of Food Science; Institute of Food Chemistry, University of Hamburg , Grindelallee 117, 20146 Hamburg, Germany.
authors.forename: Christin
authors.initials: C
authors.lastname: Fischer
authors.affiliation: Hamburg School of Food Science; Institute of Food Chemistry, University of Hamburg , Grindelallee 117, 20146 Hamburg, Germany.
authors.forename: Constanze
authors.initials: C
authors.lastname: Kallinich
authors.affiliation: Hamburg School of Food Science; Institute of Food Chemistry, University of Hamburg , Grindelallee 117, 20146 Hamburg, Germany.
authors.forename: Sven
authors.initials: S
authors.lastname: Klockmann
authors.affiliation: Hamburg School of Food Science; Institute of Food Chemistry, University of Hamburg , Grindelallee 117, 20146 Hamburg, Germany.
authors.forename: Jil
authors.initials: J
authors.lastname: Schrader
authors.affiliation: Hamburg School of Food Science; Institute of Food Chemistry, University of Hamburg , Grindelallee 117, 20146 Hamburg, Germany.
authors.forename: Markus
authors.initials: M
authors.lastname: Fischer
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000032:analysis*; D052157:Aptamers, Nucleotide / Q000737:chemistry; D005506:Food Contamination / Q000032:analysis*; D008280:Magnetics; D008892:Milk / Q000737:chemistry*; D015203:Reproducibility of Results; D013424:Sulfanilamides / Q000032:analysis*
chemical_list: D000900:Anti-Bacterial Agents; D052157:Aptamers, Nucleotide; D013424:Sulfanilamides
keywords: antibiotics; aptamer; automation; enrichment; milk; sulfanilamide