title: Screening and Analysis of Janelia FlyLight Project Enhancer-Gal4 Strains Identifies Multiple Gene Enhancers Active During Hematopoiesis in Normal and Wasp-ChallengedDrosophilaLarvae.
abstract: A GFP expression screen has been conducted on >1000 Janelia FlyLight Project enhancer-Gal4 lines to identify transcriptional enhancers active in the larval hematopoietic system. A total of 190 enhancers associated with 87 distinct genes showed activity in cells of the third instar larval lymph gland and hemolymph. That is, gene enhancers were active in cells of the lymph gland posterior signaling center (PSC), medullary zone (MZ), and/or cortical zone (CZ), while certain of the transcriptional control regions were active in circulating hemocytes. Phenotypic analyses were undertaken on 81 of these hematopoietic-expressed genes, with nine genes characterized in detail as to gain- and loss-of-function phenotypes in larval hematopoietic tissues and blood cells. These studies demonstrated the functional requirement of thecutgene for proper PSC niche formation, thehairy,Btk29A, andE2F1genes for blood cell progenitor production in the MZ domain, and thelongitudinals lacking,dFOXO,kayak,cap-n-collar, anddelilahgenes for lamellocyte induction and/or differentiation in response to parasitic wasp challenge and infestation of larvae. Together, these findings contribute substantial information to our knowledge of genes expressed during the larval stage ofDrosophilahematopoiesis and newly identify multiple genes required for this developmental process.
authors.affiliation: Department of Biological Sciences, University of Notre Dame, Indiana 46556.
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Tokusumi
authors.affiliation: Department of Biological Sciences, University of Notre Dame, Indiana 46556.
authors.forename: Yumiko
authors.initials: Y
authors.lastname: Tokusumi
authors.affiliation: Department of Biological Sciences, University of Notre Dame, Indiana 46556.
authors.forename: Mark S
authors.initials: MS
authors.lastname: Brahier
authors.affiliation: Department of Biological Sciences, University of Notre Dame, Indiana 46556.
authors.forename: Victoria
authors.initials: V
authors.lastname: Lam
authors.affiliation: Department of Biological Sciences, University of Notre Dame, Indiana 46556.
authors.forename: Jessica R
authors.initials: JR
authors.lastname: Stoller-Conrad
authors.affiliation: Department of Biological Sciences, University of Notre Dame, Indiana 46556.
authors.forename: Paul T
authors.initials: PT
authors.lastname: Kroeger
authors.affiliation: Department of Biological Sciences, University of Notre Dame, Indiana 46556 rschulz@nd.edu.
authors.forename: Robert A
authors.initials: RA
authors.lastname: Schulz
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000235:genetics; D029721:Drosophila Proteins / Q000235:genetics; D004331:Drosophila melanogaster / Q000235:genetics* / Q000469:parasitology; D050687:E2F1 Transcription Factor / Q000235:genetics; D004742:Enhancer Elements, Genetic; D051858:Forkhead Transcription Factors / Q000235:genetics; D018507:Gene Expression Regulation, Developmental; D006410:Hematopoiesis / Q000235:genetics*; D006412:Hematopoietic Stem Cells / Q000378:metabolism; D006434:Hemocytes / Q000378:metabolism; D007814:Larva / Q000235:genetics / Q000469:parasitology; D011505:Protein-Tyrosine Kinases / Q000235:genetics; D012045:Regulatory Sequences, Nucleic Acid / Q000235:genetics*; D015398:Signal Transduction / Q000235:genetics; D014863:Wasps / Q000472:pathogenicity
chemical_list: D029721:Drosophila Proteins; D050687:E2F1 Transcription Factor; C477334:FOXO protein, Drosophila; D051858:Forkhead Transcription Factors; D011505:Protein-Tyrosine Kinases; C474449:Btk29A protein, Drosophila
keywords: Drosophila hematopoiesis; blood cell-specific gene expression; gene enhancer-Gal4 line screening; larval hemocyte essential genes; wasp-challenged larvae