title: Control of cerebral ischemia with magnetic nanoparticles.
abstract: The precise manipulation of microcirculation in mice can facilitate mechanistic studies of brain injury and repair after ischemia, but this manipulation remains a technical challenge, particularly in conscious mice. We developed a technology that uses micromagnets to induce aggregation of magnetic nanoparticles to reversibly occlude blood flow in microvessels. This allowed induction of ischemia in a specific cortical region of conscious mice of any postnatal age, including perinatal and neonatal stages, with precise spatiotemporal control but without surgical intervention of the skull or artery. When combined with longitudinal live-imaging approaches, this technology facilitated the discovery of a feature of the ischemic cascade: selective loss of smooth muscle cells in juveniles but not adults shortly after onset of ischemia and during blood reperfusion.
authors.affiliation: Children's Research Institute, University of Texas Southwestern Medical Center, Dallas, Texas, USA.
authors.forename: Jie-Min
authors.initials: JM
authors.lastname: Jia
authors.affiliation: Department of Chemistry, Stanford University, Stanford, California, USA.
authors.forename: Praveen D
authors.initials: PD
authors.lastname: Chowdary
authors.affiliation: Children's Research Institute, University of Texas Southwestern Medical Center, Dallas, Texas, USA.
authors.forename: Xiaofei
authors.initials: X
authors.lastname: Gao
authors.affiliation: Children's Research Institute, University of Texas Southwestern Medical Center, Dallas, Texas, USA.
authors.forename: Bo
authors.initials: B
authors.lastname: Ci
authors.affiliation: Center for Neuroscience Discovery, University of North Texas Health Science Center, Fort Worth, Texas, USA.
authors.forename: Wenjun
authors.initials: W
authors.lastname: Li
authors.affiliation: Department of Radiology, University of Texas Southwestern Medical Center, Dallas, Texas, USA.
authors.forename: Aditi
authors.initials: A
authors.lastname: Mulgaonkar
authors.affiliation: Department of Neurology and Neurotherapeutics, University of Texas Southwestern Medical Center, Dallas, Texas, USA.
authors.forename: Erik J
authors.initials: EJ
authors.lastname: Plautz
authors.affiliation: Department of Radiology, University of Texas Southwestern Medical Center, Dallas, Texas, USA.
authors.forename: Gedaa
authors.initials: G
authors.lastname: Hassan
authors.affiliation: Department of Radiology, University of Texas Southwestern Medical Center, Dallas, Texas, USA.
authors.forename: Amit
authors.initials: A
authors.lastname: Kumar
authors.affiliation: Department of Neurology and Neurotherapeutics, University of Texas Southwestern Medical Center, Dallas, Texas, USA.
authors.forename: Ann M
authors.initials: AM
authors.lastname: Stowe
authors.affiliation: Center for Neuroscience Discovery, University of North Texas Health Science Center, Fort Worth, Texas, USA.
authors.forename: Shao-Hua
authors.initials: SH
authors.lastname: Yang
authors.affiliation: Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics-HuaZhong University of Science and Technology, Wuhan, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhou
authors.affiliation: Department of Radiology, University of Texas Southwestern Medical Center, Dallas, Texas, USA.
authors.forename: Xiankai
authors.initials: X
authors.lastname: Sun
authors.affiliation: Department of Chemistry, Stanford University, Stanford, California, USA.
authors.forename: Bianxiao
authors.initials: B
authors.lastname: Cui
authors.affiliation: Children's Research Institute, University of Texas Southwestern Medical Center, Dallas, Texas, USA.|Department of Pediatrics, University of Texas Southwestern Medical Center, Dallas, Texas, USA.|Department of Neuroscience, University of Texas Southwestern Medical Center, Dallas, Texas, USA.|Harold C. Simmons Comprehensive Cancer Center, University of Texas Southwestern Medical Center, Dallas, Texas, USA.
authors.forename: Woo-Ping
authors.initials: WP
authors.lastname: Ge
mesh_terms: D000818:Animals; D002545:Brain Ischemia / Q000139:chemically induced* / Q000188:drug therapy / Q000503:physiopathology*; D002536:Cerebral Arteries / Q000187:drug effects / Q000503:physiopathology; D002560:Cerebrovascular Circulation / Q000187:drug effects / Q000502:physiology; D004195:Disease Models, Animal; D057809:HEK293 Cells; D006624:Hippocampus / Q000187:drug effects; D006801:Humans; D008279:Magnetic Resonance Imaging / Q000379:methods; D058185:Magnetite Nanoparticles / Q000009:adverse effects* / Q000737:chemistry / Q000633:toxicity; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D008833:Microcirculation / Q000187:drug effects; D055806:Microvessels / Q000187:drug effects / Q000503:physiopathology
chemical_list: D058185:Magnetite Nanoparticles