title: Anti-Inflammatory Activity of Biomass Extracts of the Bay Mushroom, Imleria badia (Agaricomycetes), in RAW 264.7 Cells.
abstract: The popular edible bay mushroom, Imleria badia, is rich in phenolic and indole compounds, unsaturated fatty acids, and elements that have anti-inflammatory activity, including zinc. The aim of this study was to evaluate the pro- or anti-inflammatory impact of I. badia biomass extracts on RAW 264.7 cells. We analyzed the influence of I. badia extracts and/or lipopolysaccharide (LPS) cotreatment on the fatty acid profile of cell membranes. We also analyzed cyclooxygenase-2, prostaglandin E synthase, glutathione S transferase Mul, and nuclear factor (NF)-κB p50 and p65 protein expression, as well as the activity of peroxisome proliferator-activated receptor γ and NF-κB transcription factors. Incubation of cells with I. badia extracts resulted in a decreased expression of cyclooxygenase-2 and prostaglandin E2 synthase when compared with the LPS-activated macrophages. Glutathione S transferase Mul expression increased after incubation of cells with I. badia extracts. p50 And p65 NF-κB protein levels were lower in macrophages after extract treatment. Lower activity of NF-κB and higher activity of peroxisome proliferator-activated receptor γ was observed in cells treated with I. badia extracts in the presence of LPS when compared with cells activated by LPS alone. Our findings suggest the anti-inflammatory properties of I. badia biomass extracts from in vitro cultures, which could contribute to the development of new strategies in the treatment of inflammation.
authors.affiliation: Department of Pharmaceutical Botany, Faculty of Pharmacy, Jagiellonian University Medical College, Krakow, Poland.
authors.forename: Agata
authors.initials: A
authors.lastname: Grzywacz
authors.affiliation: Department of Radioligands, Faculty of Pharmacy, Jagiellonian University Medical College, Krakow, Poland.
authors.forename: Joanna Gdula
authors.initials: JG
authors.lastname: Argasinska
authors.affiliation: Department of Pharmaceutical Botany, Faculty of Pharmacy, Jagiellonian University Medical College, Krakow, Poland.
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Kala
authors.affiliation: Department of Inorganic Chemistry, Faculty of Pharmacy, Jagiellonian University Medical College, Krakow, Poland.
authors.forename: Wlodzimierz
authors.initials: W
authors.lastname: Opoka
authors.affiliation: Department of Pharmaceutical Botany, Faculty of Pharmacy, Jagiellonian University Medical College, Krakow, Poland.
authors.forename: Bozena
authors.initials: B
authors.lastname: Muszynska
mesh_terms: D000363:Agaricales / Q000737:chemistry*; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000737:chemistry / Q000494:pharmacology*; D018533:Biomass; D005786:Gene Expression Regulation / Q000187:drug effects; D008070:Lipopolysaccharides; D008264:Macrophages / Q000187:drug effects; D051379:Mice; D016328:NF-kappa B / Q000235:genetics / Q000378:metabolism; D047495:PPAR gamma / Q000235:genetics / Q000378:metabolism; D000067996:RAW 264.7 Cells
chemical_list: D000893:Anti-Inflammatory Agents; D008070:Lipopolysaccharides; D016328:NF-kappa B; D047495:PPAR gamma