title: Influences of pH and Iron Concentration on the Salivary Microbiome in Individual Humans with and without Caries.
abstract: This study aimed to identify the differences in the oral microbial communities in saliva from patients with and without caries by performing sequencing with the Illumina MiSeq platform, as well as to further assess their relationships with environmental factors (salivary pH and iron concentration). Forty-three volunteers were selected, including 21 subjects with and 22 without caries, from one village in Gansu, China. Based on 966,255 trimmed sequences and clustering at the 97% similarity level, 1,303 species-level operational taxonomic units were generated. The sequencing data for the two groups revealed that (i) particular distribution patterns (synergistic effects or competition) existed in the subjects with and without caries at both the genus and species levels and (ii) both the salivary pH and iron concentration had significant influences on the microbial community structure.
  
  
  
  The significant influences of the oral environment observed in this study increase the current understanding of the salivary microbiome in caries. These results will be useful for expanding research directions and for improving disease diagnosis, prognosis, and therapy.
authors.affiliation: Key Laboratory of Oral Diseases of Gansu Province, Key Laboratory of Stomatology of the State Ethnic Affairs Commission, Northwest University for Nationalities, Lanzhou, Gansu, China.
authors.forename: Jianye
authors.initials: J
authors.lastname: Zhou
authors.affiliation: Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang, China.
authors.forename: Nan
authors.initials: N
authors.lastname: Jiang
authors.affiliation: School of Stomatology, Lanzhou University, Lanzhou, China.
authors.forename: Zhenzhen
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou, China.
authors.forename: Longqing
authors.initials: L
authors.lastname: Li
authors.affiliation: Key Laboratory of Oral Diseases of Gansu Province, Key Laboratory of Stomatology of the State Ethnic Affairs Commission, Northwest University for Nationalities, Lanzhou, Gansu, China.
authors.forename: Jumei
authors.initials: J
authors.lastname: Zhang
authors.affiliation: School of Stomatology, Lanzhou University, Lanzhou, China.
authors.forename: Rui
authors.initials: R
authors.lastname: Ma
authors.affiliation: Key Laboratory of Oral Diseases of Gansu Province, Key Laboratory of Stomatology of the State Ethnic Affairs Commission, Northwest University for Nationalities, Lanzhou, Gansu, China.
authors.forename: Hongbing
authors.initials: H
authors.lastname: Nie
authors.affiliation: Key Laboratory of Oral Diseases of Gansu Province, Key Laboratory of Stomatology of the State Ethnic Affairs Commission, Northwest University for Nationalities, Lanzhou, Gansu, China sciuse1@163.com.
authors.forename: Zhiqiang
authors.initials: Z
authors.lastname: Li
mesh_terms: D039903:Actinobacteria / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D000328:Adult; D041963:Bacteroidetes / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D001483:Base Sequence; D004269:DNA, Bacterial / Q000235:genetics; D003731:Dental Caries / Q000382:microbiology; D000068536:Bacillota / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D045182:Fusobacteria / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007501:Iron / Q000032:analysis*; D064307:Microbiota / Q000235:genetics*; D008875:Middle Aged; D009055:Mouth / Q000382:microbiology*; D020560:Proteobacteria / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D012463:Saliva / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D013142:Spirochaeta / Q000145:classification / Q000235:genetics / Q000302:isolation & purification
chemical_list: D004269:DNA, Bacterial; D007501:Iron
keywords: caries; iron; microbial communities; pH; saliva