title: Protective effects of ginsenoside Rg1 on intestinal ischemia/reperfusion injury-induced oxidative stress and apoptosis via activation of the Wnt/β-catenin pathway.
abstract: Ginsenoside Rg1 (Rg1) is one of the major bioactive ingredients in Panax ginseng, and it attenuates inflammation and apoptosis. The aims of our study were to explore the potential of Rg1 for the treatment of intestinal I/R injury and to determine whether the protective effects of Rg1 were exerted through the Wnt/β-catenin signaling pathway. In this study, Rg1 treatment ameliorated inflammatory factors, ROS and apoptosis that were induced by intestinal I/R injury. Cell viability was increased and cell apoptosis was decreased with Rg1 pretreatment following hypoxia/reoxygenation (H/R) in the in vitro study. Rg1 activated the Wnt/β-catenin signaling pathway in both the in vivo and in vitro models, and in the in vitro study, the activation was blocked by DKK1. Our study provides evidence that pretreatment with Rg1 significantly reduces ROS and apoptosis induced by intestinal I/R injury via activation of the Wnt/β-catenin pathway. Taken together, our results suggest that Rg1 could exert its therapeutic effects on intestinal I/R injury through the Wnt/β-catenin signaling pathway and provide a novel treatment modality for intestinal I/R injury.
authors.affiliation: Department of Gastroenterology Surgery, The Dalian Municipal Central Hospital Affiliated of Dalian Medical University, Dalian 116033, PR China.
authors.forename: Guo
authors.initials: G
authors.lastname: Zu
authors.affiliation: Department of Surgical Operation, Dalian Medical University, Dalian 116044, PR China.
authors.forename: Jing
authors.initials: J
authors.lastname: Guo
authors.affiliation: Department of Neurosurgery, The Second Affiliated Hospital of Dalian Medical University, Dalian 116027, China.
authors.forename: Ningwei
authors.initials: N
authors.lastname: Che
authors.affiliation: Department of Neurology, The First Affiliated Hospital of Dalian Medical University, Dalian 116011, China.
authors.forename: Tingting
authors.initials: T
authors.lastname: Zhou
authors.affiliation: Department of Gastroenterology Surgery, The Dalian Municipal Central Hospital Affiliated of Dalian Medical University, Dalian 116033, PR China.
authors.forename: Xiangwen
authors.initials: X
authors.lastname: Zhang
authors.forename: Guangzhi
authors.initials: G
authors.lastname: Wang
authors.forename: Anlong
authors.lastname: Ji
authors.forename: Xiaofeng
authors.initials: X
authors.lastname: Tian
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D036145:Ginsenosides / Q000008:administration & dosage*; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins / Q000235:genetics; D007422:Intestines / Q000187:drug effects* / Q000473:pathology; D018384:Oxidative Stress / Q000187:drug effects*; D051381:Rats; D015427:Reperfusion Injury / Q000188:drug therapy* / Q000235:genetics / Q000473:pathology; D060449:Wnt Signaling Pathway / Q000187:drug effects
chemical_list: C501126:DKK1 protein, human; D036145:Ginsenosides; D036341:Intercellular Signaling Peptides and Proteins; C035054:ginsenoside Rg1