title: Cytotoxicity study of Piper nigrum seed mediated synthesized SnO2nanoparticles towards colorectal (HCT116) and lung cancer (A549) cell lines.
abstract: Different sized tetragonal tin oxide nanoparticles (SnO2NPs) were synthesized using Piper nigrum seed extract at three different calcination temperatures (300, 500, 900°C) and these nanoparticles (NPs) were characterized by transmission electron microscopy (TEM), X-ray diffraction (XRD), dynamic light scattering (DLS) and Fourier transform infrared spectrophotometry (FT-IR). The optical properties were studied using UV-Vis and photoluminescence (PL) spectrophotometers. The generation of reactive oxygen species (ROS) was monitored by using a fluorescence spectrophotometer and fluorescence microscope. The cytotoxicity of the synthesized SnO2NPs was checked against the colorectal (HCT116) and lung (A549) cancer cell lines and the study results show that SnO2NPs were toxic against cancer cell lines depending on their size and dose. IC50values of SnO2NPs having average particle sizes of 8.85±3.5, 12.76±3.9 and 29.29±10.9nm are 165, 174 and 208μgL-1against HCT116, while these values are 135, 157 and 187μgL-1against A549 carcinoma cell lines, respectively. The generated ROS were responsible for the cytotoxicity of SnO2NPs to the studied cancer cells and smaller size NPs generated more ROS and hence showed higher cytotoxicity over larger size NPs. The results of this study suggest that the synthesized stable nanoparticles could be a potent therapeutic agent towards cancerous cell lines.
authors.affiliation: Trace Elements Speciation Research Laboratory, Department of Chemistry, School of Advanced Sciences, VIT University, Vellore 632014, India.
authors.forename: Sai Kumar
authors.initials: SK
authors.lastname: Tammina
authors.affiliation: Trace Elements Speciation Research Laboratory, Department of Chemistry, School of Advanced Sciences, VIT University, Vellore 632014, India. Electronic address: badalmandal@vit.ac.in.
authors.forename: Badal Kumar
authors.initials: BK
authors.lastname: Mandal
authors.affiliation: Nano-food Research Group, Instrumental and Food Analysis Laboratory, School of Bio Sciences and Technology, VIT University, Vellore, Tamil Nadu, India.
authors.forename: Shivendu
authors.initials: S
authors.lastname: Ranjan
authors.affiliation: Nano-food Research Group, Instrumental and Food Analysis Laboratory, School of Bio Sciences and Technology, VIT University, Vellore, Tamil Nadu, India.
authors.forename: Nandita
authors.initials: N
authors.lastname: Dasgupta
mesh_terms: D045744:Cell Line, Tumor; D015179:Colorectal Neoplasms / Q000473:pathology*; D004354:Drug Screening Assays, Antitumor; D006801:Humans; D008175:Lung Neoplasms / Q000473:pathology*; D046529:Microscopy, Electron, Transmission; D053758:Nanoparticles / Q000737:chemistry*; D029222:Piper nigrum / Q000196:embryology*; D012639:Seeds / Q000737:chemistry*; D017971:Tin Compounds / Q000737:chemistry*; D014961:X-Ray Diffraction
chemical_list: D017971:Tin Compounds; C045358:stannic oxide
keywords: Cancerous cells; Cytotoxicity; Photoluminescence; Piper nigrum; ROS; SnO(2) NPs; Tetragonal