title: Hydrogen sulfide enhances pancreatic β-cell differentiation from human tooth under normal and glucotoxic conditions.
abstract: Glucotoxicity obstructs pancreatic differentiation from adult stem cells. The aim was to develop a novel protocol for differentiation of dental pulp stem cells (DPSCs) into pancreatic β cells and determine the effect of H2S on glucotoxicity.
  
  
  
  DPSCs were differentiated with media containing 5.5 or 25.0 mM glucose, exposed to 1 ng/ml H2S. Glucotoxicity, expression of β-cell markers, INS, PDX1 and GLUT2, and PI3K/AKT pathway were assessed.
  
  
  
  H2S exposure increased insulin and C-peptide, and protected DPSC-derived pancreatic β-like cells from glucotoxicity and upregulated INS, PDX1 and GLUT2, and genes of PI3K/AKT pathway.
  
  
  
  H2S improved effects of glucotoxicity on β-like cells via PI3K/AKT pathway. The protocol for pancreatic β-cell differentiation might have applications in regenerative medicine rather than swine pancreas transplantation.
authors.affiliation: Department of Oral Health, Nippon Dental University, School of Life Dentistry at Tokyo, 1-9-20 Chiyoda-ku, 102-8159 Tokyo, Japan.
authors.forename: Ioan Valentin
authors.initials: IV
authors.lastname: Matei
authors.affiliation: Department of Oral Health, Nippon Dental University, School of Life Dentistry at Tokyo, 1-9-20 Chiyoda-ku, 102-8159 Tokyo, Japan.
authors.forename: Hisataka
authors.initials: H
authors.lastname: Ii
authors.affiliation: Department of Oral Health, Nippon Dental University, School of Life Dentistry at Tokyo, 1-9-20 Chiyoda-ku, 102-8159 Tokyo, Japan.
authors.forename: Ken
authors.initials: K
authors.lastname: Yaegaki
mesh_terms: D015415:Biomarkers / Q000378:metabolism; D002454:Cell Differentiation / Q000187:drug effects*; D002478:Cells, Cultured; D003782:Dental Pulp / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D005947:Glucose / Q000009:adverse effects*; D006801:Humans; D006862:Hydrogen Sulfide / Q000494:pharmacology*; D007328:Insulin / Q000378:metabolism; D050417:Insulin-Secreting Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D038081:Organogenesis / Q000187:drug effects / Q000502:physiology; D016877:Oxidants / Q000494:pharmacology; D013234:Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D013549:Sweetening Agents / Q000009:adverse effects; D014070:Tooth / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism
chemical_list: D015415:Biomarkers; D007328:Insulin; D016877:Oxidants; D013549:Sweetening Agents; D005947:Glucose; D006862:Hydrogen Sulfide
keywords: glucotoxicity; hydrogen sulfide; pancreatic β-like cells