title: The importance of vial composition in HPLC analysis: An unusual case of phosphorous pseudorotation.
abstract: Glass HPLC vials are ubiquitous in analytical laboratories and vendors have developed many varieties to meet the various needs of scientists. As such there may be multiple types of vials being used simultaneously in a single laboratory without much consideration as to which is best suited for analytical method development and validation. This study highlights the possibility of vial composition as a potential factor that impacts solution stability. Here we describe a case where the type of HPLC vial used results in an interesting phosphorous pseudorotation driven by the mild alkalinity of glass.
authors.affiliation: Department of Process and Analytical Chemistry, Merck Research Laboratories, Merck & Co., Inc., Rahway, NJ, 07065, United States. Electronic address: rebecca_arvary@merck.com.
authors.forename: Rebecca
authors.initials: R
authors.lastname: Arvary
authors.affiliation: Department of Process and Analytical Chemistry, Merck Research Laboratories, Merck & Co., Inc., Rahway, NJ, 07065, United States.
authors.forename: Ian
authors.initials: I
authors.lastname: Mangion
mesh_terms: D002626:Chemistry, Pharmaceutical / Q000379:methods*; D004349:Drug Packaging / Q000379:methods / Q000592:standards*; D004355:Drug Stability; D005898:Glass / Q000032:analysis / Q000737:chemistry* / Q000592:standards*; D010758:Phosphorus / Q000032:analysis / Q000737:chemistry*
chemical_list: D010758:Phosphorus
keywords: Glass; HPLC; Phosphoramidite; Pseudorotation; Purity analysis; Stability