title: Self-Assembled Tb3+Complex Probe for Quantitative Analysis of ATP during Its Enzymatic Hydrolysis via Time-Resolved Luminescence in Vitro and in Vivo.
abstract: To more accurately assess the pathways of biological systems, a probe is needed that may respond selectively to adenosine triphosphate (ATP) for both in vitro and in vivo detection modes. We have developed a luminescence probe that can provide real-time information on the extent of ATP, ADP, and AMP by virtue of the luminescence and luminescence lifetime observed from a supramolecular polymer based on a C3symmetrical terpyridine complex with Tb3+(S1-Tb). The probe shows remarkable selective luminescence enhancement in the presence of ATP compared to other phosphate-displaying nucleotides including adenosine diphosphate (ADP), adenosine monophosphate (AMP), guanosine triphosphate (GTP), thymidine triphosphate (TTP), H2PO4-(Pi), and pyrophosphate (PPi). In addition, the time-resolved luminescence lifetime and luminescence spectrum of S1-Tb could facilitate the quantitative measurement of the exact amount of ATP and similarly ADP and AMP within living cells. The time-resolved luminescence lifetime of S1-Tb could also be used to quantitatively monitor the amount of ATP, ADP, and AMP in vitro following the enzymatic hydrolysis of ATP. The long luminescence lifetime, which was observed into the millisecond range, makes this S1-Tb-based probe particularly attractive for monitoring biological ATP levels in vivo, because any short lifetime background fluorescence arising from the complex molecular environment may be easily eliminated.
authors.affiliation: Department of Chemistry and Research Institute of Natural Sciences, Gyeongsang National University , Jinju, Korea.
authors.forename: Sung Ho
authors.initials: SH
authors.lastname: Jung
authors.affiliation: Department of Chemistry and Research Institute of Natural Sciences, Gyeongsang National University , Jinju, Korea.
authors.forename: Ka Young
authors.initials: KY
authors.lastname: Kim
authors.affiliation: Department of Chemistry and Research Institute of Natural Sciences, Gyeongsang National University , Jinju, Korea.
authors.forename: Ji Ha
authors.initials: JH
authors.lastname: Lee
authors.affiliation: Department of Chemistry and Research Institute of Natural Sciences, Gyeongsang National University , Jinju, Korea.
authors.forename: Cheol Joo
authors.initials: CJ
authors.lastname: Moon
authors.affiliation: Department of Chemistry, Kyung Hee University , Seoul 130-701, Korea.
authors.forename: Noh Soo
authors.initials: NS
authors.lastname: Han
authors.affiliation: Department of Life Science, Ewha Womans University , Seoul 120-750, Korea.
authors.forename: Su-Jin
authors.initials: SJ
authors.lastname: Park
authors.affiliation: Department of Life Science, Ewha Womans University , Seoul 120-750, Korea.
authors.forename: Dongmin
authors.initials: D
authors.lastname: Kang
authors.affiliation: Department of Chemistry, Kyung Hee University , Seoul 130-701, Korea.
authors.forename: Jae Kyu
authors.initials: JK
authors.lastname: Song
authors.affiliation: Department of Chemistry and Research Institute of Natural Sciences, Gyeongsang National University , Jinju, Korea.
authors.forename: Shim Sung
authors.identifier: http://orcid.org/0000-0002-4638-5466
authors.initials: SS
authors.lastname: Lee
authors.affiliation: Department of Chemistry and Research Institute of Natural Sciences, Gyeongsang National University , Jinju, Korea.
authors.forename: Myong Yong
authors.identifier: http://orcid.org/0000-0001-5729-5418
authors.initials: MY
authors.lastname: Choi
authors.affiliation: Department of Chemical Engineering and Institute of Nanoscience and Technology, Hanyang University , Seoul, Korea.
authors.forename: Justyn
authors.initials: J
authors.lastname: Jaworski
authors.affiliation: Department of Chemistry and Research Institute of Natural Sciences, Gyeongsang National University , Jinju, Korea.
authors.forename: Jong Hwa
authors.identifier: http://orcid.org/0000-0002-8936-2272
authors.initials: JH
authors.lastname: Jung
mesh_terms: D000255:Adenosine Triphosphate; D006160:Guanosine Triphosphate; D006868:Hydrolysis; D049449:Luminescence; D010710:Phosphates
chemical_list: D010710:Phosphates; D006160:Guanosine Triphosphate; D000255:Adenosine Triphosphate
keywords: adenosine triphosphate (ATP); chemoprobe; lanthanide; lifetime; supramolecular polymer