title: Genetic Diversity and Relationships of 24 Strains of Genus Auricularia (Agaricomycetes) Assessed Using SRAP Markers.
abstract: Auricularia species are well known for their traditional edible and medicinal importance. In this study, sequence-related amplified polymorphism (SRAP) markers were used to evaluate the genetic diversity and relationships of 24 Auricularia strains obtained from different ecological regions of China. A total of 402 polymorphic loci were detected using 11 SRAP primer combinations with 100% polymorphic loci. The similarity coefficient ranged from 0.662 to 0.903, with an average of 0.73. Cluster analysis using the pair-group method using arithmetic average, based on the data from SRAP loci amplified by 11 primer pairs, showed that 24 Auricularia strains were distinguished into 4 groups with 73% similarity. The results of principal coordinate analysis were in accordance with pair-group method using arithmetic average clustering, and the first, most informative coordinate accounted for 74.38% of all the variations, which indicated a high level of genetic diversity among Auricularia strains. In general, SRAP markers provide a powerful tool to discriminate Auricularia strains and to evaluate their genetic relationships.
authors.affiliation: Heilongjiang Agricultural Economy Vocational College, Mudanjiang, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Du
authors.affiliation: Heilongjiang Agricultural Economy Vocational College, Mudanjiang, China.
authors.forename: Tian-Xu
authors.initials: TX
authors.lastname: Cao
authors.affiliation: Institute of Microbiology, Beijing Forestry University, Beijing, People's Republic of China.
authors.forename: Bao-Kai
authors.initials: BK
authors.lastname: Cui
authors.affiliation: Institute of Microbiology, Beijing Forestry University, Beijing, China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Yuan
authors.affiliation: Institute of Microbiology, Beijing Forestry University, Beijing, China.
authors.forename: Yu-Cheng
authors.initials: YC
authors.lastname: Dai
mesh_terms: D001487:Basidiomycota / Q000145:classification / Q000235:genetics*; D002681:China; D016000:Cluster Analysis; D017931:DNA Primers / Q000235:genetics; D004271:DNA, Fungal / Q000235:genetics; D005819:Genetic Markers / Q000235:genetics; D014644:Genetic Variation; D011110:Polymorphism, Genetic
chemical_list: D017931:DNA Primers; D004271:DNA, Fungal; D005819:Genetic Markers