title: Comparative immunomorphology of testicular Sertoli and sertoliform tumors.
abstract: Sertoli cell (SC) and sertoliform tumors of the testis are very uncommon; for this reason their differential diagnosis and classification can be challenging. We applied an extensive immunophenotypic panel that included androgenic hormones, enzymes and receptors, neuroendocrine, lineage and genitourinary markers to a series of these lesions to determine if and which immunostains can aid in their diagnostic workup. Study cases included: 2 androgen insensitivity syndrome-associated SC adenomas, 3 SC tumors (SCT) not otherwise specified (SCT-NOS), 3 sclerosing SCT, 2 large cell calcifying SCT, 1 SCT with heterologous sarcomatous elements, 1 malignant SCT, and 1 sertoliform rete testis adenoma (sertoliform RTA). We found that SCT-NOS and variants with sclerosis showed a phenotype akin to atrophic seminiferous tubules characterized by gain of expression of pankeratin, calretinin, CD56, which are negative in normal SC. Distinctive phenotypes were identified in: sclerosing SCT: androgen receptors (AR) + (strong)/PAX2/PAX8+ (subset)/S100+/inhibin-; large cell calcifying SCT: calretinin+ (strong)/S100+/AR-; sertoliform RTA: PAX2/PAX8+/pankeratin+/inhibin-. Androgenic hormones and enzymes did not show diagnostic utility. A panel of calretinin, inhibin, pankeratin, S100, PAX2/PAX8, and AR consistently allowed distinction between variants of Sertoli and sertoliform tumors.
authors.affiliation: Departments of Pathology and Laboratory Medicine, Veterans Administration Health Care Service, Minneapolis, MN 55417. Electronic address: Hector.Mesa@va.gov.
authors.forename: Hector
authors.initials: H
authors.lastname: Mesa
authors.affiliation: Departments of Pathology and Laboratory Medicine, University of Minnesota School of Medicine, Minneapolis, MN 55455.
authors.forename: Scott
authors.initials: S
authors.lastname: Gilles
authors.affiliation: Departments of Pathology and Laboratory Medicine, University of Minnesota School of Medicine, Minneapolis, MN 55455; Departments of Pathology and Laboratory Medicine, Hospital Pathology Associates, Minneapolis, MN 55407.
authors.forename: Milton W
authors.initials: MW
authors.lastname: Datta
authors.affiliation: Departments of Pathology and Laboratory Medicine, University of Minnesota School of Medicine, Minneapolis, MN 55455.
authors.forename: Paari
authors.initials: P
authors.lastname: Murugan
authors.affiliation: Departments of Pathology and Laboratory Medicine, Veterans Administration Health Care Service, Minneapolis, MN 55417.
authors.forename: Wendy
authors.initials: W
authors.lastname: Larson
authors.affiliation: Departments of Pathology and Laboratory Medicine, Veterans Administration Health Care Service, Minneapolis, MN 55417.
authors.forename: Susan
authors.initials: S
authors.lastname: Dachel
authors.affiliation: Departments of Pathology and Laboratory Medicine, Veterans Administration Health Care Service, Minneapolis, MN 55417; Departments of Pathology and Laboratory Medicine, University of Minnesota School of Medicine, Minneapolis, MN 55455.
authors.forename: Juan C
authors.initials: JC
authors.lastname: Manivel
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000032:analysis*; D001706:Biopsy; D019070:Cell Lineage; D062625:Cystadenofibroma / Q000276:immunology / Q000473:pathology; D003537:Cystadenoma / Q000276:immunology / Q000473:pathology; D003937:Diagnosis, Differential; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D008910:Minnesota; D010641:Phenotype; D011237:Predictive Value of Tests; D012707:Sertoli Cell Tumor / Q000276:immunology* / Q000473:pathology*; D012708:Sertoli Cells / Q000276:immunology* / Q000473:pathology*; D013736:Testicular Neoplasms / Q000276:immunology* / Q000473:pathology*; D055815:Young Adult
chemical_list: D014408:Biomarkers, Tumor
keywords: Classification; Immunohistochemistry; Rete testis; Sertoli cell tumor; Sertoli cells; Testicular neoplasms