title: Single nucleotide polymorphisms of IFNγ (+874 A/T) and IFNγR1 (-56 C/T) in Iranian patients with TB.
abstract: Two important genes for controlling TB are IFNγ and IFNγR1. However, little information exists regarding genetic susceptibility of the Iranian TB population.
  
  
  
  We investigated the single nucleotide polymorphisms (SNPs) in genes of IFNγ (+874 A/T) and IFNγR1 (-56 C/T) and serum level of IFNγ and their influence on TB in patients; 300 patients with TB and 300 healthy controls were enrolled in this study. PCR-restriction fragment length polymorphism was used to identify SNPs and serum level of IFNγ was measured by ELISA.
  
  
  
  The allelic and the genotypic form of IFNγ+874 A/T SNP of the studied population were not significant (p>0.05). Allele T frequencies of IFNγR1 -56 C/T promoter region in patients with pulmonary TB (PTB) or extrapulmonary TB (EPTB) were significantly greater than allele C. The -56 TT motif of IFNγR1 is associated with both forms of TB (p<0.05). The serum level of IFNγ was significantly higher in patients with TB than in controls, but there was no significant difference between serum level of IFNγ and the studied genotypes (p>0.05).
  
  
  
  The cause of active TB in the patients seems to be due to the lack of effective IFNγ function or the lack of effective signaling connection between IFNγ and its receptor in presence of -56 C/T polymorphism in promoter region of IFNγR1 gene.
authors.affiliation: Immunogenetics Research Center, Mazandaran University of Medical Sciences, Sari, Iran, Biotechnology Department, Pasteur Institute of Iran, Tehran, Iran.
authors.forename: Elham
authors.initials: E
authors.lastname: Beiranvand
authors.affiliation: Immunogenetics Research Center, Mazandaran University of Medical Sciences, Sari, Iran abedianks@razi.tums.ac.ir.
authors.forename: Saeid
authors.initials: S
authors.lastname: Abediankenari
authors.affiliation: Department of Medical Biotechnology, Pasteur Institute of Iran, Tehran, Iran.
authors.forename: Samira
authors.initials: S
authors.lastname: Valiyari
authors.affiliation: Booalisina Hospital, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran.
authors.forename: Mohammad Sadegh
authors.initials: MS
authors.lastname: Rezaei
authors.affiliation: Immunology Department, Pasteur Institute of Iran, Tehran, Iran.
authors.forename: Mosayeb
authors.initials: M
authors.lastname: Rostamian
authors.affiliation: Bam University of Medical Sciences, Bam, Iran.
authors.forename: Behnoush
authors.initials: B
authors.lastname: Beiranvand
authors.affiliation: Sabzevar University of Medical Science, Sabzevar, Iran.
authors.forename: Ali
authors.initials: A
authors.lastname: Khaligh
authors.affiliation: Biochemistry Department, Pasteur Institute of Iran, Tehran, Iran.
authors.forename: Soghra
authors.initials: S
authors.lastname: Khani
mesh_terms: D000328:Adult; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D007371:Interferon-gamma / Q000235:genetics*; D007492:Iran; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D011401:Promoter Regions, Genetic / Q000235:genetics; D017471:Receptors, Interferon / Q000235:genetics*; D014376:Tuberculosis / Q000235:genetics* / Q000276:immunology; D000096743:Interferon gamma Receptor
chemical_list: D007371:Interferon-gamma; D017471:Receptors, Interferon; D000096743:Interferon gamma Receptor
keywords: +874 A/T; Iran; Tuberculosis; −56 C/T