title: Evaluation of Relationships between Growth Rate, Tree Size, Lignocellulose Composition, and Enzymatic Saccharification in InterspecificCorymbiaHybrids and Parental Taxa.
abstract: In order for a lignocellulosic bioenergy feedstock to be considered sustainable, it must possess a high rate of growth to supply biomass for conversion. Despite the desirability of a fast growth rate for industrial application, it is unclear what effect growth rate has on biomass composition or saccharification. We characterized Klason lignin, glucan, and xylan content with response to growth inCorymbiainterspecific F1 hybrid families (HF) and parental speciesCorymbia torellianaandC. citriodorasubspeciesvariegataand measured the effects on enzymatic hydrolysis from hydrothermally pretreated biomass. Analysis of biomass composition withinCorymbiapopulations found similar amounts of Klason lignin content (19.7-21.3%) among parental and hybrid populations, whereas glucan content was clearly distinguished withinC. citriodorasubspeciesvariegata(52%) and HF148 (60%) as compared to other populations (28-38%). Multiple linear regression indicates that biomass composition is significantly impacted by tree size measured at the same age, with Klason lignin content increasing with diameter breast height (DBH) (+0.12% per cm DBH increase), and glucan and xylan typically decreasing per DBH cm increase (-0.7 and -0.3%, respectively). Polysaccharide content withinC. citriodorasubspeciesvariegataand HF-148 were not significantly affected by tree size. High-throughput enzymatic saccharification of hydrothermally pretreated biomass found significant differences amongCorymbiapopulations for total glucose production from biomass, with parentalCorymbia torellianaand hybrids HF-148 and HF-51 generating the highest amounts of glucose (~180 mg/g biomass, respectively), with HF-51 undergoing the most efficient glucan-to-glucose conversion (74%). Based on growth rate, biomass composition, and further optimization of enzymatic saccharification yield, high productionCorymbiahybrid trees are potentially suitable for fast-rotation bioenergy or biomaterial production.
authors.affiliation: Queensland Alliance for Agriculture and Food Innovation, University of Queensland St. Lucia, QLD, Australia.
authors.forename: Adam L
authors.initials: AL
authors.lastname: Healey
authors.affiliation: Forest Industries Research Centre, University of the Sunshine CoastMaroochydore, QLD, Australia; Forestry & Biosciences, Agri-Science Queensland, Department of Agriculture and FisheriesGympie, QLD, Australia.
authors.forename: David J
authors.initials: DJ
authors.lastname: Lee
authors.affiliation: Prozess Technologie St. Louis, MO, USA.
authors.forename: Jason S
authors.initials: JS
authors.lastname: Lupoi
authors.affiliation: Joint BioEnergy Institute, Lawrence Berkeley National Laboratory Emeryville, CA, USA.
authors.forename: Gabriella
authors.initials: G
authors.lastname: Papa
authors.affiliation: Joint BioEnergy Institute, Lawrence Berkeley National LaboratoryEmeryville, CA, USA; Biological and Engineering Sciences Center, Sandia National LaboratoriesLivermore, CA, USA.
authors.forename: Joel M
authors.initials: JM
authors.lastname: Guenther
authors.affiliation: Gruppo Ricicla - Biomass and Bioenergy Laboratory, DiSAA, University of Milan Milan, Italy.
authors.forename: Luca
authors.initials: L
authors.lastname: Corno
authors.affiliation: Gruppo Ricicla - Biomass and Bioenergy Laboratory, DiSAA, University of Milan Milan, Italy.
authors.forename: Fabrizio
authors.initials: F
authors.lastname: Adani
authors.affiliation: Joint BioEnergy Institute, Lawrence Berkeley National LaboratoryEmeryville, CA, USA; Biological and Engineering Sciences Center, Sandia National LaboratoriesLivermore, CA, USA.
authors.forename: Seema
authors.initials: S
authors.lastname: Singh
authors.affiliation: Joint BioEnergy Institute, Lawrence Berkeley National LaboratoryEmeryville, CA, USA; Biological and Engineering Sciences Center, Sandia National LaboratoriesLivermore, CA, USA.
authors.forename: Blake A
authors.initials: BA
authors.lastname: Simmons
authors.affiliation: Queensland Alliance for Agriculture and Food Innovation, University of Queensland St. Lucia, QLD, Australia.
authors.forename: Robert J
authors.initials: RJ
authors.lastname: Henry
keywords: Corymbia; biofuels; eucalypt; glucan; growth rate; lignin; saccharification; xylan