title: Clarification of the Antagonistic Effect of the Lipopeptides Produced by Bacillus amyloliquefaciens BPD1 against Pyricularia oryzae via In Situ MALDI-TOF IMS Analysis.
abstract: This study tried to clarify the antagonistic effect of the lipopeptides secreted byBacillus amyloliquefaciensstrain BPD1 (Ba-BPD1) againstPyricularia oryzaeCavara (PO). To determine the major antifungal lipopeptides effective against PO, single and dual cultures were carried out in solid-state media. The matrix-assisted laser desorption/ionization-time of flight imaging mass spectrometry (MALDI-TOF IMS) was used to identify the most effective lipopeptide in situ. Meanwhile, the morphology of pathogen fungi treated with lipopeptides was observed via the SEM. Of the three lipopeptide families, surfactin, iturin, and fengycin, the last was identified as the most effective for inhibiting mycelium growth and conidial germination of PO. The conidia and hyphae of fengycin-treated PO were shown to become deformed and tumorous under exposure. This study provides insights into the antagonistic effect of Ba-BPD1 against fungal phytopathogens. Such insights are helpful in the development of reagents for biological control applications.
authors.affiliation: Department of Chemical Engineering, National Chung Hsing University, Taichung 40227, Taiwan. colvin@taifer.com.tw.|Biopesticides Division, Taiwan Agricultural Chemicals and Toxic Substances Research Institute, Council of Agriculture, Taichung 41358, Taiwan. colvin@taifer.com.tw.
authors.forename: Jen-Hung
authors.initials: JH
authors.lastname: Liao
authors.affiliation: Agricultural Biotechnology Research Center, Academia Sinica, Taipei 11529, Taiwan. jasper28@gate.sinica.edu.tw.
authors.forename: Pi-Yu
authors.initials: PY
authors.lastname: Chen
authors.affiliation: Agricultural Biotechnology Research Center, Academia Sinica, Taipei 11529, Taiwan. ylyang@gate.sinica.edu.tw.
authors.forename: Yu-Liang
authors.initials: YL
authors.lastname: Yang
authors.affiliation: Department of Chemical Engineering, National Chung Hsing University, Taichung 40227, Taiwan. sherrykan0402@gmail.com.
authors.forename: Shu-Chen
authors.initials: SC
authors.lastname: Kan
authors.affiliation: Biopesticides Division, Taiwan Agricultural Chemicals and Toxic Substances Research Institute, Council of Agriculture, Taichung 41358, Taiwan. hsiehf@tactri.gov.tw.
authors.forename: Feng-Chia
authors.initials: FC
authors.lastname: Hsieh
authors.affiliation: Department of Chemical Engineering, National Chung Hsing University, Taichung 40227, Taiwan. ycliu@dragon.nchu.edu.tw.
authors.forename: Yung-Chang
authors.initials: YC
authors.lastname: Liu
mesh_terms: D000898:Antibiosis; D000935:Antifungal Agents / Q000494:pharmacology*; D001203:Ascomycota / Q000187:drug effects* / Q000254:growth & development; D000069977:Bacillus amyloliquefaciens / Q000378:metabolism*; D001426:Bacterial Proteins / Q000096:biosynthesis / Q000494:pharmacology*; D055666:Lipopeptides / Q000096:biosynthesis / Q000494:pharmacology*; D008826:Microbial Sensitivity Tests; D025282:Mycelium / Q000187:drug effects / Q000254:growth & development; D010802:Phylogeny; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013172:Spores, Fungal / Q000187:drug effects / Q000254:growth & development
chemical_list: D000935:Antifungal Agents; D001426:Bacterial Proteins; D055666:Lipopeptides
keywords: Bacillus amyloliquefaciens BPD1; MALDI-TOF IMS; SEM; fengycin; lipopeptides