title: miR-429 Inhibits Differentiation and Promotes Proliferation in Porcine Preadipocytes.
abstract: MicroRNAs (miRNAs) are crucial regulatory molecules for adipogenesis. They contribute to the controlling of proliferation and differentiation of preadipocytes. Previous studies revealed an important role of miR-429 in cell invasion, migration, and apoptosis. Our previous work has shown that the expression of miR-429 in subcutaneous fat can be observed in newly born (3-day-old) Rongchang piglets rather than their adult counterparts (180-day-old). This expression pattern suggests that miR-429 might be functionally related to postnatal adipogenesis. However, we currently lack a mechanistic understanding of miR-429 within the context of preadipocyte differentiation. In this study, we investigated the function of miR-429 in porcine subcutaneous and intramuscular preadipocyte proliferation and differentiation. In our porcine preadipocyte differentiation model, miR-429 expression decreased remarkably upon adipogenic induction. Overexpression of miR-429 notably down-regulated the expression of adipogenic marker genes:PPARγ,aP2,FASand impaired the triglyceride accumulation, while the expression of lipolytic geneATGLwas not affected. In addition, we observed that miR-429 significantly promoted the proliferation of porcine preadipocytes. We also found that miR-429 could directly bind to the 3'-UTRs ofKLF9andp27, which have been well documented to promote preadipocyte differentiation and repress cell cycle progression. Taken together, our data support a novel role of miR-429 in regulating porcine preadipocyte differentiation and proliferation, andKLF9andp27are potent targets of miR-429 during these processes.
authors.affiliation: Laboratory of Animal Fat Deposition & Muscle Development, College of Animal Science and Technology, Northwest A&F University, Xianyang 712100, China. py9101@163.com.
authors.forename: Ying
authors.initials: Y
authors.lastname: Peng
authors.affiliation: Laboratory of Animal Fat Deposition & Muscle Development, College of Animal Science and Technology, Northwest A&F University, Xianyang 712100, China. ffchen03@sina.com.|School of Life Sciences, Southwest Forestry University, Kunming 650224, China. ffchen03@sina.com.
authors.forename: Fen-Fen
authors.initials: FF
authors.lastname: Chen
authors.affiliation: Laboratory of Animal Fat Deposition & Muscle Development, College of Animal Science and Technology, Northwest A&F University, Xianyang 712100, China. jingjingdehnaa@163.com.
authors.forename: Jing
authors.initials: J
authors.lastname: Ge
authors.affiliation: Laboratory of Animal Fat Deposition & Muscle Development, College of Animal Science and Technology, Northwest A&F University, Xianyang 712100, China. jiayuzhulam@163.com.
authors.forename: Jia-Yu
authors.initials: JY
authors.lastname: Zhu
authors.affiliation: Laboratory of Animal Fat Deposition & Muscle Development, College of Animal Science and Technology, Northwest A&F University, Xianyang 712100, China. xineshi@nwsuaf.edu.cn.
authors.forename: Xin-E
authors.initials: XE
authors.lastname: Shi
authors.affiliation: Laboratory of Animal Fat Deposition & Muscle Development, College of Animal Science and Technology, Northwest A&F University, Xianyang 712100, China. nice.lixiao@gmail.com.
authors.forename: Xiao
authors.initials: X
authors.lastname: Li
authors.affiliation: Laboratory of Animal Fat Deposition & Muscle Development, College of Animal Science and Technology, Northwest A&F University, Xianyang 712100, China. yutaiyong310@nwsuaf.edu.cn.
authors.forename: Tai-Yong
authors.initials: TY
authors.lastname: Yu
authors.affiliation: Laboratory of Animal Fat Deposition & Muscle Development, College of Animal Science and Technology, Northwest A&F University, Xianyang 712100, China. yz97721@gmail.com.
authors.forename: Gui-Yan
authors.initials: GY
authors.lastname: Chu
authors.affiliation: Laboratory of Animal Fat Deposition & Muscle Development, College of Animal Science and Technology, Northwest A&F University, Xianyang 712100, China. gsyang999@hotmail.com.
authors.forename: Gong-She
authors.initials: GS
authors.lastname: Yang
mesh_terms: D017667:Adipocytes / Q000166:cytology* / Q000378:metabolism*; D050156:Adipogenesis / Q000235:genetics / Q000502:physiology; D000818:Animals; D002454:Cell Differentiation / Q000235:genetics / Q000502:physiology; D049109:Cell Proliferation / Q000235:genetics / Q000502:physiology; D035683:MicroRNAs / Q000235:genetics*; D047495:PPAR gamma / Q000235:genetics / Q000378:metabolism; D018809:Proliferating Cell Nuclear Antigen / Q000235:genetics / Q000378:metabolism; D013552:Swine
chemical_list: D035683:MicroRNAs; D047495:PPAR gamma; D018809:Proliferating Cell Nuclear Antigen; C413374:p27 antigen
keywords: KLF9; differentiation; miR-429; p27; porcine intramuscular preadipocytes; porcine subcutaneous preadipocytes; proliferation