title: Identification of microRNAs as biomarkers for cholangiocarcinoma detection: A diagnostic meta-analysis.
abstract: BACKGROUND
  Cholangiocarcinoma (CCA) is a common biliary malignancy that results in high mortality and poor survival rates. The diagnostic capacity of miRNAs in CCA detection is still controversial. Current meta-analysis evaluate the evidence on the capacity of miRNAs for CCA diagnosis.
  
  
  METHODS
  A systematic literature search for relevant articles in PubMed, Embase and Web of science database up to 10 April 2016 was conducted. The pooled sensitivity, specificity, positive and negative likelihood ratios (PLR and NLR, respectively), diagnostic odds ratio (DOR) and area under the curve (AUC) were pooled to assess the overall diagnostic value of miRNA. Subgroup analysis was further applied to explore the between-study heterogeneity. Publication bias was assessed with Deeks' funnel plot. Statistical analysis was carried out using RevMan 5.2 and MetaDisc 1.4.
  
  
  RESULTS
  A total of 501 patients and 248 controls from 14 studies in 8 articles were included in this meta-analysis. The pooled sensitivity and specialty were 0.83 (0.80-0.86) and 0.79 (0.75-0.83), respectively. The pooled PLR was 3.96 (2.64-5.94), NLR was 0.25 (0.19-0.34), DOR was 18.1 (9.79-33.5) and pooled AUC was 0.88 (0.85-0.90). Subgroup analyses indicated that the serum miRNA assays showed highly accurate CCA diagnosis. The diagnostic capacity was more accurate in Caucasians than in Asians.
  
  
  CONCLUSIONS
  The miRNA assays are promising diagnostic tools for CCA detection with moderate accuracy. However, further prospective studies were needed to confirm this results before clinical application.
authors.affiliation: Department of General Surgery, Nanjing First Hospital, Nanjing Medical University, 210006 Nanjing, China.
authors.forename: J
authors.initials: J
authors.lastname: Zhou
authors.affiliation: Department of General Surgery, Nanjing First Hospital, Nanjing Medical University, 210006 Nanjing, China.
authors.forename: Z
authors.initials: Z
authors.lastname: Liu
authors.affiliation: Department of General Surgery, Nanjing First Hospital, Nanjing Medical University, 210006 Nanjing, China.
authors.forename: S
authors.initials: S
authors.lastname: Yang
authors.affiliation: Key Laboratory on Living Donor Liver Transplantation, Ministry of Health, Department of liver surgery, First Affiliated Hospital of Nanjing Medical University, 210029 Nanjing, China. Electronic address: zjlixic@126.com.
authors.forename: X
authors.initials: X
authors.lastname: Li
mesh_terms: D044466:Asian People; D001650:Bile Duct Neoplasms / Q000097:blood* / Q000235:genetics; D014408:Biomarkers, Tumor / Q000097:blood*; D018281:Cholangiocarcinoma / Q000097:blood* / Q000235:genetics; D006801:Humans; D035683:MicroRNAs / Q000097:blood*; D016017:Odds Ratio; D012680:Sensitivity and Specificity; D044465:White People
chemical_list: D014408:Biomarkers, Tumor; D035683:MicroRNAs