title: Excellent storage stability and sensitive detection of neurotoxin quinolinic acid.
abstract: Quinolinic acid (QA) is a metabolite of tryptophan degradation obtained through kynurenine pathway, produced naturally in the mammalian brain as well as in the human cerebrospinal fluid. The presence of QA ~10-40µM is a clear indicator of many neurological disorders as well as deficiency of vitamin B6in human being. In the present work; rapid, sensitive and cost-effective bio-electrodes were prepared to detect the trace amount of endogenous neurotoxin (QA). Cyclic voltammetry (CV) and differential pulse voltammetry (DPV) studies were carried out to measure the electrochemical response of the fabricated bio-electrodes as a function of QA concentrations. These devices were found to exhibit desirable sensitivity of ~7.86mAμM-1cm-2in wide concentration range (6.5μM-65mM). The lower detection limit of this device is as low as 6.5μM and it has excellent storage stability of ~30 days. The capability of the proposed electrochemical bio-sensor was also checked to detect QA in the real samples (human serum). These results reveal that the use of this electrochemical bio-sensor may provide a potential platform for the detection of QA in the real samples for the prior detection of many diseases.
authors.affiliation: Department of Physics, Institute of Science, Banaras Hindu University, Varanasi 221005, India.
authors.forename: Ranjana
authors.initials: R
authors.lastname: Singh
authors.affiliation: Department of Zoology, Institute of Science, Banaras Hindu University, Varanasi 221005, India.
authors.forename: Sunayana
authors.initials: S
authors.lastname: Kashyap
authors.affiliation: Department of Biotechnology, Delhi Technological University, Main Bawana Road, Delhi 110042, India.
authors.forename: Suveen
authors.initials: S
authors.lastname: Kumar
authors.affiliation: Department of Physics, Institute of Science, Banaras Hindu University, Varanasi 221005, India.
authors.forename: Shiju
authors.initials: S
authors.lastname: Abraham
authors.affiliation: Department of Physics, Institute of Science, Banaras Hindu University, Varanasi 221005, India.
authors.forename: Tejendra K
authors.initials: TK
authors.lastname: Gupta
authors.affiliation: School of Biotechnology, Institute of Science, Banaras Hindu University, Varanasi 221005, India.
authors.forename: Arvind M
authors.initials: AM
authors.lastname: Kayastha
authors.affiliation: Department of Biotechnology, Delhi Technological University, Main Bawana Road, Delhi 110042, India.
authors.forename: Bansi D
authors.initials: BD
authors.lastname: Malhotra
authors.affiliation: Department of Zoology, Institute of Science, Banaras Hindu University, Varanasi 221005, India. Electronic address: pssaxena@rediffmail.com.
authors.forename: Preeti Suman
authors.initials: PS
authors.lastname: Saxena
authors.affiliation: Department of Physics, Institute of Science, Banaras Hindu University, Varanasi 221005, India. Electronic address: anchalbhu@gmail.com.
authors.forename: Anchal
authors.initials: A
authors.lastname: Srivastava
authors.affiliation: Department of Physics, Institute of Science, Banaras Hindu University, Varanasi 221005, India. Electronic address: ranjanksingh65@rediffmail.com.
authors.forename: Ranjan K
authors.initials: RK
authors.lastname: Singh
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation*; D055664:Electrochemical Techniques / Q000295:instrumentation*; D004566:Electrodes; D004800:Enzymes, Immobilized / Q000737:chemistry; D004867:Equipment Design; D006108:Graphite / Q000737:chemistry; D006801:Humans; D057230:Limit of Detection; D009498:Neurotoxins / Q000032:analysis / Q000097:blood; D010084:Oxidation-Reduction; D010430:Pentosyltransferases / Q000737:chemistry; D017378:Quinolinic Acid / Q000032:analysis / Q000097:blood*; D000098553:Nicotinate-Nucleotide Diphosphorylase (Carboxylating)
chemical_list: D004800:Enzymes, Immobilized; D009498:Neurotoxins; D006108:Graphite; D010430:Pentosyltransferases; D000098553:Nicotinate-Nucleotide Diphosphorylase (Carboxylating); D017378:Quinolinic Acid
keywords: Bio-electrodes; Enzyme; Neurotoxin; QPRT; Quinolinic Acid