title: Immunoreactivity evaluation of a new recombinant chimeric protein againstBrucellain the murine model.
abstract: OBJECTIVE
  Brucellosis is an important health problem in developing countries and no vaccine is available for the prevention of infection in humans. Because of clinically infectious diseases and their economic consequences in human and animals, designing a proper vaccine againstBrucellais desirable. In this study, we evaluated the immune responses induced by a designed recombinant chimera protein in murine model.
  
  
  METHODS
  Three immunodominant antigens ofBrucellahave been characterized as potential immunogenic and protective antigens including: trigger factor (TF), Omp31 and Bp26 were fused together by EAAAK linkers to produce a chimera (structure were designedin silico), which was synthesized, cloned, and expressed inE. coliBL21 (DE3). The purification of recombinant protein was performed using Ni-NTA agarose. SDS-PAGE and anti-His antibody was used for confirmation purified protein (Western blot). BALB/c immunization was performed by purified protein and adjuvant, and sera antibody levels were measured by ELISA. otted.
  
  
  RESULTS
  SDS-PAGE and Western blotting results indicated the similarity ofin silicodesigning andin vitroexperiments. ELISA result proved that the immunized sera of mice contain high levels of antibodies (IgG) against recombinant chimeric protein.
  
  
  CONCLUSIONS
  The recombinant chimeric protein could be a potential antigen candidate for the development of a subunit vaccine againstBrucella.
authors.affiliation: Department of Microbiology, Kerman University of Medical Sciences, Kerman, Iran.
authors.forename: Abbas
authors.initials: A
authors.lastname: Abdollahi
authors.affiliation: Department of Microbiology, Kerman University of Medical Sciences, Kerman, Iran.
authors.forename: Shahla
authors.initials: S
authors.lastname: Mansouri
authors.affiliation: Applied Microbiology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran.
authors.forename: Jafar
authors.initials: J
authors.lastname: Amani
authors.affiliation: Molecular Biology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran.
authors.forename: Mahdi
authors.initials: M
authors.lastname: Fasihi-Ramandi
authors.affiliation: Department of Microbiology, Kerman University of Medical Sciences, Kerman, Iran.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Moradi
keywords: Brucella; Immunity; Recombinant; Vaccine