title: Bioelectronic Nose Using Odorant Binding Protein-Derived Peptide and Carbon Nanotube Field-Effect Transistor for the Assessment of Salmonella Contamination in Food.
abstract: Salmonella infection is the one of the major causes of food borne illnesses including fever, abdominal pain, diarrhea, and nausea. Thus, early detection of Salmonella contamination is important for our healthy life. Conventional detection methods for the food contamination have limitations in sensitivity and rapidity; thus, the early detection has been difficult. Herein, we developed a bioelectronic nose using a carbon nanotube (CNT) field-effect transistor (FET) functionalized with Drosophila odorant binding protein (OBP)-derived peptide for easy and rapid detection of Salmonella contamination in ham. 3-Methyl-1-butanol is known as a specific volatile organic compound, generated from the ham contaminated with Salmonella. We designed and synthesized the peptide based on the sequence of the Drosophila OBP, LUSH, which specifically binds to alcohols. The C-terminus of the synthetic peptide was modified with three phenylalanine residues and directly immobilized onto CNT channels using the π-π interaction. The p-type properties of FET were clearly maintained after the functionalization using the peptide. The biosensor detected 1 fM of 3-methyl-1-butanol with high selectivity and successfully assessed Salmonella contamination in ham. These results indicate that the bioelectronic nose can be used for the rapid detection of Salmonella contamination in food.
authors.affiliation: Interdisciplinary Program for Bioengineering, Seoul National University , Seoul 151-742, Korea.
authors.forename: Manki
authors.initials: M
authors.lastname: Son
authors.affiliation: Department of Biophysics and Chemical Biology, Seoul National University , Seoul 151-742, Korea.
authors.forename: Daesan
authors.initials: D
authors.lastname: Kim
authors.affiliation: School of Chemical and Biological Engineering, Seoul National University , Seoul 151-742, Korea.
authors.forename: Jinkyung
authors.initials: J
authors.lastname: Kang
authors.affiliation: School of Chemical and Biological Engineering, Seoul National University , Seoul 151-742, Korea.
authors.forename: Jong Hyun
authors.initials: JH
authors.lastname: Lim
authors.affiliation: School of Chemical and Biological Engineering, Seoul National University , Seoul 151-742, Korea.
authors.forename: Seung Hwan
authors.initials: SH
authors.lastname: Lee
authors.affiliation: Bio-MAX Institute, Seoul National University , Seoul 151-818, Korea.
authors.forename: Hwi Jin
authors.initials: HJ
authors.lastname: Ko
authors.affiliation: Department of Physics and Astronomy and Institute of Applied Physics, Seoul National University , Seoul 151-747, Korea.
authors.forename: Seunghun
authors.initials: S
authors.lastname: Hong
authors.affiliation: Interdisciplinary Program for Bioengineering, Seoul National University , Seoul 151-742, Korea.|School of Chemical and Biological Engineering, Seoul National University , Seoul 151-742, Korea.|Bio-MAX Institute, Seoul National University , Seoul 151-818, Korea.|Advanced Institutes of Convergence Technology , Suwon, Gyeonggi-do 443-270, Korea.
authors.forename: Tai Hyun
authors.initials: TH
authors.lastname: Park
mesh_terms: D062609:Electronic Nose; D005506:Food Contamination / Q000032:analysis*; D037742:Nanotubes, Carbon / Q000737:chemistry*; D010455:Peptides / Q000737:chemistry*; D018035:Receptors, Odorant / Q000737:chemistry*; D012475:Salmonella / Q000302:isolation & purification*; D014173:Transistors, Electronic
chemical_list: D037742:Nanotubes, Carbon; D010455:Peptides; D018035:Receptors, Odorant; C045648:odorant-binding protein