title: Antichaperone activity and heme degradation effect of methyl tert-butyl ether (MTBE) on normal and diabetic hemoglobins.
abstract: Because of the extensive use of methyl tert-butyl ether (MTBE) as an additive to increase the octane quality of gasoline, the environmental pollution by this compound has increased in recent decades. Environmental release of MTBE may lead to its entry to the blood stream through inhalation or drinking of contaminated water, and its interactions with biological molecules such as proteins. The present study was proposed to comparatively investigate the interactions of MTBE with hemoglobin (Hb) from diabetic and nondiabetic individuals using various spectroscopic methods including UV-visible, fluorescence, chemiluminescence, and circular dichroism. These results demonstrated the effects of MTBE on heme degradation of Hb and the reaction of these degradation products with water generating reactive oxygen species. Interaction of Hb with MTBE enhanced its aggregation rate and decreased lag time, indicating the antichaperone activity of MTBE upon interaction with Hb. Furthermore, the diabetic Hb showed more severe effects of MTBE, including heme degradation, reactive oxygen species production, unfolding, and antichaperone behavior than the nondiabetic Hb. The results from molecular docking suggested that the special interaction site of MTBE in the vicinity of Hb heme group is responsible for heme degradation.
authors.affiliation: Institute of Biochemistry and Biophysics, University of Tehran, Tehran, Iran.|Center of Excellence in Biothermodynamics, University of Tehran, Tehran, Iran.
authors.forename: Ismaeil Hossein
authors.initials: IH
authors.lastname: Najdegerami
authors.affiliation: Institute of Biochemistry and Biophysics, University of Tehran, Tehran, Iran.
authors.forename: Parvaneh
authors.initials: P
authors.lastname: Maghami
authors.affiliation: Institute of Biochemistry and Biophysics, University of Tehran, Tehran, Iran.|Center of Excellence in Biothermodynamics, University of Tehran, Tehran, Iran.
authors.forename: Vahid
authors.initials: V
authors.lastname: Sheikh-Hasani
authors.affiliation: Department of Physical Chemistry, School of Chemistry, College of Science, University of Tehran, Tehran, Iran.
authors.forename: Ghader
authors.initials: G
authors.lastname: Hosseinzadeh
authors.affiliation: Department of Ophthalmology and Visual Sciences, University of Wisconsin School of Medicine and Public Health, Madison, WI, USA.|Department of Biomedical Engineering, University of Wisconsin School of Medicine and Public Health, Madison, WI, USA.
authors.forename: Nader
authors.initials: N
authors.lastname: Sheibani
authors.affiliation: Institute of Biochemistry and Biophysics, University of Tehran, Tehran, Iran.|Center of Excellence in Biothermodynamics, University of Tehran, Tehran, Iran.
authors.forename: Ali A
authors.initials: AA
authors.lastname: Moosavi-Movahedi
mesh_terms: D000393:Air Pollutants / Q000009:adverse effects* / Q000494:pharmacology; D000818:Animals; D002942:Circular Dichroism; D003920:Diabetes Mellitus; D006418:Heme / Q000737:chemistry*; D006454:Hemoglobins / Q000737:chemistry / Q000187:drug effects*; D006801:Humans; D049449:Luminescence; D008738:Methyl Ethers / Q000009:adverse effects* / Q000737:chemistry / Q000494:pharmacology; D051379:Mice; D008958:Models, Molecular; D062105:Molecular Docking Simulation; D058767:Protein Unfolding; D017382:Reactive Oxygen Species / Q000378:metabolism
chemical_list: D000393:Air Pollutants; D006454:Hemoglobins; D008738:Methyl Ethers; D017382:Reactive Oxygen Species; C043243:methyl tert-butyl ether; D006418:Heme
keywords: ROS production; antichaperone; diabetes; heme degradation; hemoglobin; methyl tert-butyl ether (MTBE)