title: Operating principles of rotary molecular motors: differences between F1and V1motors.
abstract: Among the many types of bioenergy-transducing machineries, F- and V-ATPases are unique bio- and nano-molecular rotary motors. The rotational catalysis of F1-ATPase has been investigated in detail, and molecular mechanisms have been proposed based on the crystal structures of the complex and on extensive single-molecule rotational observations. Recently, we obtained crystal structures of bacterial V1-ATPase (A3B3and A3B3DF complexes) in the presence and absence of nucleotides. Based on these new structures, we present a novel model for the rotational catalysis mechanism of V1-ATPase, which is different from that of F1-ATPases.
authors.affiliation: Department of Biological Science and Technology, Tokyo University of Science, Tokyo 125-8585, Japan.
authors.forename: Ichiro
authors.initials: I
authors.lastname: Yamato
authors.affiliation: Laboratory of Molecular Physiology and Genetics, Faculty of Agriculture, Ehime University, Matsuyama, Ehime 790-8566, Japan.
authors.forename: Yoshimi
authors.initials: Y
authors.lastname: Kakinuma
authors.affiliation: Department of Chemistry, Graduate School of Science, Chiba University, Chiba 263-8522, Japan; Molecular Chirality Research Center, Chiba University, Chiba 263-8522, Japan; JST, PRESTO, Chiba 263-8522, Japan.
authors.forename: Takeshi
authors.initials: T
authors.lastname: Murata
keywords: ATP; Crystal structure; F-ATPase; V-ATPase; molecular mechanism