title: Molecular characterization of a new soybean-infecting member of the genus Nepovirus identified by high-throughput sequencing.
abstract: The complete nucleotide sequence of a new soybean-infecting member of the genus Nepovirus (provisionally named "soybean latent spherical virus" [SLSV]) was identified by high-throughput sequencing of RNAs from soybean leaf samples from North Dakota, USA. The sequences of RNAs 1 (8,190 nt) and 2 (5,788 nt) were completed by rapid amplification of cDNA ends. Each contained a single long open reading frame and a 3' nontranslated region of greater than 1,500 nt. The predicted amino acid sequences of the two ORFs were most closely related to nepoviruses in subgroup C. Full-length cDNAs of RNAs 1 and 2 were cloned and used to inoculate soybean plants, which did not display obvious symptoms. These results suggest that SLSV represents a new species in the genus Nepovirus.
authors.affiliation: Department of Crop Sciences, University of Illinois, Urbana, IL, USA.
authors.forename: Tuba
authors.initials: T
authors.lastname: Yasmin
authors.affiliation: Department of Plant Pathology, North Dakota State University, Fargo, ND, USA.
authors.forename: Berlin D
authors.initials: BD
authors.lastname: Nelson
authors.affiliation: Department of Crop Sciences, University of Illinois, Urbana, IL, USA.
authors.forename: Houston A
authors.initials: HA
authors.lastname: Hobbs
authors.affiliation: United States Department of Agriculture, Agricultural Research Service, Urbana, IL, USA.
authors.forename: Nancy K
authors.initials: NK
authors.lastname: McCoppin
authors.affiliation: Department of Crop Sciences, University of Illinois, Urbana, IL, USA.
authors.forename: Kris N
authors.initials: KN
authors.lastname: Lambert
authors.affiliation: Department of Crop Sciences, University of Illinois, Urbana, IL, USA. leslie.domier@ars.usda.gov.|United States Department of Agriculture, Agricultural Research Service, Urbana, IL, USA. leslie.domier@ars.usda.gov.
authors.forename: Leslie L
authors.initials: LL
authors.lastname: Domier
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D016679:Genome, Viral; D059014:High-Throughput Nucleotide Sequencing; D008969:Molecular Sequence Data; D017860:Nepovirus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification* / Q000502:physiology; D016366:Open Reading Frames; D010802:Phylogeny; D010935:Plant Diseases / Q000821:virology*; D012367:RNA, Viral / Q000235:genetics; D016415:Sequence Alignment; D013025:Glycine max / Q000821:virology*; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics
chemical_list: D012367:RNA, Viral; D014764:Viral Proteins