title: Expression Level of theDREB2-Type Gene, Identified with Amplifluor SNP Markers, Correlates with Performance, and Tolerance to Dehydration in Bread Wheat Cultivars from Northern Kazakhstan.
abstract: A panel of 89 local commercial cultivars of bread wheat was tested in field trials in the dry conditions of Northern Kazakhstan. Two distinct groups of cultivars (six cultivars in each group), which had the highest and the lowest grain yield under drought were selected for further experiments. A dehydration test conducted on detached leaves indicated a strong association between rates of water loss in plants from the first group with highest grain yield production in the dry environment relative to the second group. Modern high-throughput Amplifluor Single Nucleotide Polymorphism (SNP) technology was applied to study allelic variations in a series of drought-responsive genes using 19 SNP markers. Genotyping of an SNP in theTaDREB5(DREB2-type) gene using the Amplifluor SNP marker KATU48 revealed clear allele distribution across the entire panel of wheat accessions, and distinguished between the two groups of cultivars with high and low yield under drought. Significant differences in expression levels ofTaDREB5were revealed by qRT-PCR. Most wheat plants from the first group of cultivars with high grain yield showed slight up-regulation in the TaDREB5 transcript in dehydrated leaves. In contrast, expression ofTaDREB5in plants from the second group of cultivars with low grain yield was significantly down-regulated. It was found that SNPs did not alter the amino acid sequence of TaDREB5 protein. Thus, a possible explanation is that alternative splicing and up-stream regulation ofTaDREB5may be affected by SNP, but these hypotheses require additional analysis (and will be the focus of future studies).
authors.affiliation: School of Agriculture, Food and Wine, Faculty of Sciences, University of AdelaideUrrbrae, SA, Australia; School of Biological Sciences, Flinders University, Bedford ParkSA, Australia.
authors.forename: Yuri
authors.initials: Y
authors.lastname: Shavrukov
authors.affiliation: Faculty of Agronomy, S. Seifullin Kazakh AgroTechnical University Astana Kazakhstan.
authors.forename: Aibek
authors.initials: A
authors.lastname: Zhumalin
authors.affiliation: Faculty of Agronomy, S. Seifullin Kazakh AgroTechnical University Astana Kazakhstan.
authors.forename: Dauren
authors.initials: D
authors.lastname: Serikbay
authors.affiliation: Faculty of Agronomy, S. Seifullin Kazakh AgroTechnical University Astana Kazakhstan.
authors.forename: Makpal
authors.initials: M
authors.lastname: Botayeva
authors.affiliation: Faculty of Agronomy, S. Seifullin Kazakh AgroTechnical University Astana Kazakhstan.
authors.forename: Ainur
authors.initials: A
authors.lastname: Otemisova
authors.affiliation: National Agricultural Research Education Centre Astana, Kazakhstan.
authors.forename: Aiman
authors.initials: A
authors.lastname: Absattarova
authors.affiliation: Karaganda Research Institute of Plant Industry and Breeding Karaganda, Kazakhstan.
authors.forename: Grigoriy
authors.initials: G
authors.lastname: Sereda
authors.affiliation: Karaganda Research Institute of Plant Industry and Breeding Karaganda, Kazakhstan.
authors.forename: Sergey
authors.initials: S
authors.lastname: Sereda
authors.affiliation: Faculty of Agronomy, S. Seifullin Kazakh AgroTechnical University Astana Kazakhstan.
authors.forename: Vladimir
authors.initials: V
authors.lastname: Shvidchenko
authors.affiliation: Faculty of Agronomy, S. Seifullin Kazakh AgroTechnical University Astana Kazakhstan.
authors.forename: Arysgul
authors.initials: A
authors.lastname: Turbekova
authors.affiliation: Faculty of Agronomy, S. Seifullin Kazakh AgroTechnical University Astana Kazakhstan.
authors.forename: Satyvaldy
authors.initials: S
authors.lastname: Jatayev
authors.affiliation: School of Agriculture, Food and Wine, Faculty of Sciences, University of Adelaide Urrbrae, SA, Australia.
authors.forename: Sergiy
authors.initials: S
authors.lastname: Lopato
authors.affiliation: School of Biological Sciences, Flinders University, Bedford Park SA, Australia.
authors.forename: Kathleen
authors.initials: K
authors.lastname: Soole
authors.affiliation: School of Agriculture, Food and Wine, Faculty of Sciences, University of Adelaide Urrbrae, SA, Australia.
authors.forename: Peter
authors.initials: P
authors.lastname: Langridge
keywords: Amplifluor SNP; DREB2; bread wheat; dehydration; gene expression; genotyping; grain yield