title: Salidroside Protects Lipopolysaccharide-Induced Acute Lung Injury in Mice.
abstract: Salidroside (SDS) has been reported to have anti-inflammatory properties. The objective of this study was to investigate the protective effect of SDS on lipopolysaccharide (LPS)-induced acute lung injury (ALI) in mice. BALB/c mice were pretreated with SDS 1 hour before intranasal instillation of LPS. Seven hours after LPS administration, the myeloperoxidase in histology of lungs, lung wet/dry ratio, and inflammatory cells in the bronchoalveolar lavage fluid (BALF) were determined. The levels of pro-inflammatory cytokines, tumor necrosis factor α (TNF-α), interleukin-1β (IL 1β), and IL-6 in the BALF were measured by enzyme-linked immunosorbent assay. The expression of Toll-like receptor 4 (TLR4), inhibitor of nuclear factor-kappa B (IκB-α), and nuclear factor-kappa B (NF-κB) p65 was detected by Western blot. The SDS reduced the inflammatory cells in BALF, decreased the wet/dry ratio of lungs, attenuated the LPS-induced histological alterations in the lung, and inhibited the production of TNF-α, IL-1β, and IL-6. Western blot showed that SDS efficiently inhibited the phosphorylation of IκB-α, p65 NF-κB, and the expression of TLR4. These data show that the anti-inflammatory effects of SDS (at least 20 mg/kg) against LPS-induced ALI due to its ability to inhibit TLR4 mediated the NF-κB signaling pathways. The SDS may represent a novel strategy for treating LPS-induced ALI.
authors.affiliation: Department of Emergency, The First Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou, China.
authors.forename: Rufeng
authors.initials: R
authors.lastname: Lu
authors.affiliation: Institute of Materia Medicines, Zhejiang Academy of Medical Sciences, Hangzhou, China.
authors.forename: Yueguo
authors.initials: Y
authors.lastname: Wu
authors.affiliation: Key Laboratory of Experimental Animal and Safety Evaluation, Zhejiang Academy of Medical Sciences, Hangzhou, China.
authors.forename: Honggang
authors.initials: H
authors.lastname: Guo
authors.affiliation: Department of Emergency, The First Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou, China.
authors.forename: Xiaomin
authors.initials: X
authors.lastname: Huang
keywords: Toll-like receptor 4; acute lung injury; lipopolysaccharide; nuclear factor-κB; salidroside