title: Effect of Spirogyra neglecta on the early stages of 1, 2-dimethylhydrazine-induced colon carcinogenesis in rats.
abstract: This study focused on the chemopreventive effects of Spirogyra neglecta extract (SNE) and dried S. neglecta mixed diet on the early stages of 1,2-dimethylhydrazine (DMH)-induced colon carcinogenesis in rats. Male Wistar rats were injected with DMH to initiate aberrant crypt foci (ACF) formation. In the initiation stage, SNE significantly decreased the number of ACF in the colon of DMH-treated rats. Rats that received a low dose of SNE showed enhanced activity of several detoxifying and antioxidant enzymes. In the postinitiation stage, a low dose of SNE significantly decreased the number of ACF in the colon of DMH-treated rats. It significantly reduced the number of proliferating cell nuclear antigen-positive cells and increased the number of apoptotic cells in colonic crypts. S. neglecta thus inhibited the development of the early stages of DMH-induced colon carcinogenesis in rats by modulation of xenobiotic metabolizing enzymes and inhibition of cell proliferation as well as induction of apoptosis.
authors.affiliation: Department of Biochemistry, Faculty of Medicine.
authors.forename: Sirinya
authors.initials: S
authors.lastname: Taya
authors.affiliation: Department of Radiologic Technology, Faculty of Associated Medical Sciences.
authors.forename: Tarika
authors.initials: T
authors.lastname: Thumvijit
authors.affiliation: Department of Biochemistry, Faculty of Medicine.
authors.forename: Teera
authors.initials: T
authors.lastname: Chewonarin
authors.affiliation: Research Affairs, Faculty of Veterinary Medicine, Chiang Mai University, Chiang Mai, Thailand.
authors.forename: Charatda
authors.initials: C
authors.lastname: Punvittayagul
authors.affiliation: Department of Biochemistry, Faculty of Medicine.
authors.forename: Rawiwan
authors.initials: R
authors.lastname: Wongpoomchai
mesh_terms: D019813:1,2-Dimethylhydrazine / Q000633:toxicity; D058739:Aberrant Crypt Foci / Q000139:chemically induced / Q000473:pathology / Q000517:prevention & control*; D000818:Animals; D016588:Anticarcinogenic Agents / Q000494:pharmacology / Q000627:therapeutic use*; D017209:Apoptosis / Q000187:drug effects; D001189:Aryl Hydrocarbon Hydroxylases / Q000378:metabolism; D002273:Carcinogens / Q000633:toxicity; D049109:Cell Proliferation / Q000187:drug effects; D003106:Colon / Q000187:drug effects / Q000473:pathology; D003110:Colonic Neoplasms / Q000139:chemically induced / Q000473:pathology / Q000517:prevention & control*; D006801:Humans; D008297:Male; D009367:Neoplasm Staging; D009374:Neoplasms, Experimental / Q000139:chemically induced / Q000517:prevention & control; D010936:Plant Extracts / Q000494:pharmacology / Q000627:therapeutic use*; D051381:Rats; D017208:Rats, Wistar; D058131:Spirogyra / Q000737:chemistry*; D016896:Treatment Outcome
chemical_list: D016588:Anticarcinogenic Agents; D002273:Carcinogens; D010936:Plant Extracts; D001189:Aryl Hydrocarbon Hydroxylases; D019813:1,2-Dimethylhydrazine