title: Loss of Fractalkine Signaling Exacerbates Axon Transport Dysfunction in a Chronic Model of Glaucoma.
abstract: Neurodegeneration in glaucoma results in decline and loss of retinal ganglion cells (RGCs), and is associated with activation of myeloid cells such as microglia and macrophages. The chemokine fractalkine (FKN or Cx3cl1) mediates communication from neurons to myeloid cells. Signaling through its receptor Cx3cr1 has been implicated in multiple neurodegenerative diseases, but the effects on neuronal pathology are variable. Since it is unknown how FKN-mediated crosstalk influences RGC degeneration in glaucoma, we assessed this in a chronic mouse model, DBA/2J. We analyzed a DBA/2J substrain deficient in Cx3cr1, and compared compartmentalized RGC degeneration and myeloid cell responses to those in standard DBA/2J mice. We found that loss of FKN signaling exacerbates axon transport dysfunction, an early event in neurodegeneration, with a significant increase in RGCs with somal accumulation of the axonal protein phosphorylated neurofilament, and reduced retinal expression of genes involved in axon transport, Kif1b, and Atp8a2. There was no change in the loss of Brn3-positive RGCs, and no difference in the extent of damage to the proximal optic nerve, suggesting that the loss of fractalkine signaling primarily affects axon transport. Since Cx3cr1 is specifically expressed in myeloid cells, we assessed changes in retinal microglial number and activation, changes in gene expression, and the extent of macrophage infiltration. We found that loss of fractalkine signaling led to innate immune changes within the retina, including increased infiltration of peripheral macrophages and upregulated nitric oxide synthase-2 (Nos-2) expression in myeloid cells, which contributes to the production of NO and can promote axon transport deficits. In contrast, resident retinal microglia appeared unchanged either in number, morphology, or expression of the myeloid activation marker ionized calcium binding adaptor molecule 1 (Iba1). There was also no significant increase in the proinflammatory gene interleukin 1 beta (Il1β). We conclude that loss of fractalkine signaling causes a selective worsening of axon transport dysfunction in RGCs, which is linked to enhanced Nos-2 expression in myeloid cells. Our findings suggest that distinct mechanisms may contribute to different aspects of RGC decline in glaucoma, with axonal transport selectively altered after loss of Cx3cr1 in microglia and/or macrophages.
authors.affiliation: Departments of Neurobiology and Anatomy, University of Utah Salt Lake City, UT, USA.
authors.forename: Kevin T
authors.initials: KT
authors.lastname: Breen
authors.affiliation: Departments of Neurobiology and Anatomy, University of Utah Salt Lake City, UT, USA.
authors.forename: Sarah R
authors.initials: SR
authors.lastname: Anderson
authors.affiliation: Departments of Neurobiology and Anatomy, University of Utah Salt Lake City, UT, USA.
authors.forename: Michael R
authors.initials: MR
authors.lastname: Steele
authors.affiliation: Department of Ophthalmology and Visual Sciences, Vanderbilt University Nashville, TN, USA.
authors.forename: David J
authors.initials: DJ
authors.lastname: Calkins
authors.affiliation: Departments of Neurobiology and Anatomy, University of Utah Salt Lake City, UT, USA.
authors.forename: Alejandra
authors.initials: A
authors.lastname: Bosco
authors.affiliation: Departments of Neurobiology and Anatomy, University of Utah Salt Lake City, UT, USA.
authors.forename: Monica L
authors.initials: ML
authors.lastname: Vetter
keywords: Cx3cr1; DBA/2J; glaucoma; macrophage; microglia; neurodegeneration; retina; retinal ganglion cell