title: Neutrophil-derived microparticles are released into the coronary circulation following percutaneous coronary intervention in acute coronary syndrome patients.
abstract: To evaluate (i) local coronary and systemic levels of microparticles (MP) in acute coronary syndrome (ACS) and stable angina pectoris (SAP) patients and (ii) their release after plaque disruption with percutaneous coronary intervention (PCI). MP are small vesicles originating from plasma membranes of cells after activation or apoptosis and are implicated in the pathogenesis of atherosclerosis. Neutrophils play a role in plaque destabilization and shed neutrophil-derived MP that have the potential to drive significant proinflammatory and thrombotic downstream effects. Eight ACS and eight SAP patients were included. Coronary sinus (CS) samples pre-intervention (CS1), 45 s following balloon angioplasty (CS2) and at 45 s intervals following stent deployment (CS3, CS4 and CS5), together with peripheral vein samples, pre- and post-PCI were analysed for neutrophil-derived (CD66b+), endothelial-derived (CD144+), platelet-derived (CD41a+), monocyte-derived (CD14+) and apoptotic (Annexin V+) MP. ELISA for interleukin (IL)-6, myeloperoxidase (MPO) and P-selectin was also performed. CD66b+ MP levels were similar in both groups pre-intervention. Post-PCI, CS levels rose significantly in ACS but not SAP patients (ACS area under the curve (AUC): 549 ± 83, SAP AUC: 24 ± 29, P<0.01). CS CD41a+, CD144+, CD14+ and Annexin V+ MP levels did not differ between groups. Acute neutrophil-derived MP release post-PCI occurs in ACS compared with stable patients, likely to be reflective of plaque MP content in vulnerable lesions.
authors.affiliation: Department of Cardiology, Royal Prince Alfred Hospital, Sydney, New South Wales, Australia.|Sydney Medical School, The University of Sydney, New South Wales, Australia.|División de Enfermedades Cardiovasculares, Pontificia Universidad Católica de Chile, Santiago, Chile.
authors.forename: Gonzalo J
authors.initials: GJ
authors.lastname: Martínez
authors.affiliation: Department of Cardiology, Royal Prince Alfred Hospital, Sydney, New South Wales, Australia.|Sydney Medical School, The University of Sydney, New South Wales, Australia.|Heart Research Institute, Sydney, New South Wales, Australia.
authors.forename: Jennifer Y
authors.initials: JY
authors.lastname: Barraclough
authors.affiliation: Heart Research Institute, Sydney, New South Wales, Australia.
authors.forename: Shirley
authors.initials: S
authors.lastname: Nakhla
authors.affiliation: Heart Research Institute, Sydney, New South Wales, Australia.
authors.forename: Vivian
authors.initials: V
authors.lastname: Kienzle
authors.affiliation: Sydney Medical School, The University of Sydney, New South Wales, Australia.|Heart Research Institute, Sydney, New South Wales, Australia.
authors.forename: Stacy
authors.initials: S
authors.lastname: Robertson
authors.affiliation: Institut National de la Santé et de la Recherche Médicale (INSERM), Paris Cardiovascular Research Center, Paris, France.|Division of Cardiovascular Medicine, University of Cambridge, Addenbrooke's Hospital, Cambridge, United Kingdom.
authors.forename: Ziad
authors.initials: Z
authors.lastname: Mallat
authors.affiliation: Department of Cardiology, Royal Prince Alfred Hospital, Sydney, New South Wales, Australia.|Sydney Medical School, The University of Sydney, New South Wales, Australia.|Heart Research Institute, Sydney, New South Wales, Australia.
authors.forename: David S
authors.initials: DS
authors.lastname: Celermajer
authors.affiliation: Department of Cardiology, Royal Prince Alfred Hospital, Sydney, New South Wales, Australia sanjay.patel@hri.org.au.|Sydney Medical School, The University of Sydney, New South Wales, Australia.|Heart Research Institute, Sydney, New South Wales, Australia.
authors.forename: Sanjay
authors.initials: S
authors.lastname: Patel
mesh_terms: D054058:Acute Coronary Syndrome / Q000097:blood / Q000276:immunology / Q000601:surgery*; D000368:Aged; D060050:Angina, Stable / Q000097:blood / Q000276:immunology / Q000601:surgery*; D015906:Angioplasty, Balloon, Coronary; D015703:Antigens, CD / Q000097:blood; D015815:Cell Adhesion Molecules / Q000097:blood; D055252:Cell-Derived Microparticles / Q000276:immunology*; D003324:Coronary Artery Disease / Q000276:immunology* / Q000628:therapy; D003326:Coronary Circulation; D005260:Female; D058851:GPI-Linked Proteins / Q000097:blood; D006801:Humans; D015850:Interleukin-6 / Q000097:blood; D008297:Male; D008875:Middle Aged; D009504:Neutrophils / Q000276:immunology*; D019007:P-Selectin / Q000097:blood; D009195:Peroxidase / Q000097:blood; D058226:Plaque, Atherosclerotic / Q000276:immunology / Q000601:surgery
chemical_list: D015703:Antigens, CD; C504120:CEACAM8 protein, human; D015815:Cell Adhesion Molecules; D058851:GPI-Linked Proteins; D015850:Interleukin-6; D019007:P-Selectin; D009195:Peroxidase
keywords: Acute Coronary Syndromes; Microparticles; Neutrophils