title: Intracellular Staphylococcus aureus Control by Virulent Bacteriophages within MAC-T Bovine Mammary Epithelial Cells.
abstract: Bacteriophages (phages) are known to effectively kill extracellular multiplying bacteria. The present study demonstrated that phages penetrated bovine mammary epithelial cells and cleared intracellular Staphylococcus aureus in a time-dependent manner. In particular, phage vB_SauM_JS25 reached the nucleus within 3 h postincubation. The phages had an endocytotic efficiency of 12%. This ability to kill intracellular host bacteria suggests the utility of phage-based therapies and may protect patients from recurrent infection and treatment failure.
authors.affiliation: Key Laboratory of Control Technology and Standard for Agro-product Safety and Quality Ministry of Agriculture, Key Laboratory of Food Quality and Safety of Jiangsu Province-State Key Laboratory Breeding Base, Institute of Food Quality and Safety, Jiangsu Academy of Agricultural Sciences, Nanjing, China.
authors.forename: Lili
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Control Technology and Standard for Agro-product Safety and Quality Ministry of Agriculture, Key Laboratory of Food Quality and Safety of Jiangsu Province-State Key Laboratory Breeding Base, Institute of Food Quality and Safety, Jiangsu Academy of Agricultural Sciences, Nanjing, China.
authors.forename: Lichang
authors.initials: L
authors.lastname: Sun
authors.affiliation: Key Laboratory of Control Technology and Standard for Agro-product Safety and Quality Ministry of Agriculture, Key Laboratory of Food Quality and Safety of Jiangsu Province-State Key Laboratory Breeding Base, Institute of Food Quality and Safety, Jiangsu Academy of Agricultural Sciences, Nanjing, China.
authors.forename: Ruicheng
authors.initials: R
authors.lastname: Wei
authors.affiliation: Key Laboratory of Control Technology and Standard for Agro-product Safety and Quality Ministry of Agriculture, Key Laboratory of Food Quality and Safety of Jiangsu Province-State Key Laboratory Breeding Base, Institute of Food Quality and Safety, Jiangsu Academy of Agricultural Sciences, Nanjing, China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Gao
authors.affiliation: Key Laboratory of Control Technology and Standard for Agro-product Safety and Quality Ministry of Agriculture, Key Laboratory of Food Quality and Safety of Jiangsu Province-State Key Laboratory Breeding Base, Institute of Food Quality and Safety, Jiangsu Academy of Agricultural Sciences, Nanjing, China.
authors.forename: Tao
authors.initials: T
authors.lastname: He
authors.affiliation: Central Laboratory, Jiangsu Academy of Agricultural Science, Nanjing, China.
authors.forename: Cunfa
authors.initials: C
authors.lastname: Xu
authors.affiliation: Key Laboratory of Control Technology and Standard for Agro-product Safety and Quality Ministry of Agriculture, Key Laboratory of Food Quality and Safety of Jiangsu Province-State Key Laboratory Breeding Base, Institute of Food Quality and Safety, Jiangsu Academy of Agricultural Sciences, Nanjing, China.
authors.forename: Xianjin
authors.initials: X
authors.lastname: Liu
authors.affiliation: Key Laboratory of Control Technology and Standard for Agro-product Safety and Quality Ministry of Agriculture, Key Laboratory of Food Quality and Safety of Jiangsu Province-State Key Laboratory Breeding Base, Institute of Food Quality and Safety, Jiangsu Academy of Agricultural Sciences, Nanjing, China wangran2001@126.com.
authors.forename: Ran
authors.initials: R
authors.lastname: Wang
mesh_terms: D000818:Animals; D001435:Bacteriophages / Q000502:physiology*; D002417:Cattle; D002460:Cell Line; D002467:Cell Nucleus / Q000382:microbiology / Q000821:virology; D004847:Epithelial Cells / Q000382:microbiology* / Q000821:virology*; D008321:Mammary Glands, Animal / Q000166:cytology*; D018613:Microscopy, Confocal; D013211:Staphylococcus aureus / Q000502:physiology*
keywords: MAC-T cells; Staphylococcus aureus; bacteriophages