title: M-type phospholipase A2receptor (PLA2R) glomerular staining in pediatric idiopathic membranous nephropathy.
abstract: Identifying M-type phospholipase A2receptor (PLA2R) is a landmark breakthrough for understanding adult idiopathic membranous nephropathy (iMN). However, potential roles for PLA2R in pediatric iMN have not been well investigated.
  
  
  
  A total of 34 pediatric iMN patients who underwent kidney biopsy between 1972 and 2015 were enrolled in this study. The study cohort consisted of 15 children aged from 3 to 9 years and 19 aged from 10 to 15 years. In all cases, secondary causes of MN, including infections, autoimmune diseases, and others, were ruled out. We examined PLA2R glomerular staining in stored, formalin-fixed, paraffin-embedded kidney biopsy samples. Kidney biopsy specimens obtained from an adult patient with iMN and an adult patient with lupus-associated MN were also examined to assess our PLA2R staining procedure.
  
  
  
  Granular staining of PLA2R along glomerular capillary loops was present in two patients: an 11-year-old girl and 12-year-old boy identified during a school urine screening test and who presented with mild proteinuria at the time of biopsy. Interestingly, the intensity of PLA2R glomerular staining in these patients was weaker than that of a PLA2R-positive adult iMN patient. There were no PLA2R-positive patients among our cohort of children younger than 10 years.
  
  
  
  This preliminary study suggests PLA2R may play a role in some adolescent and preteen iMN patients but may be less frequently associated with iMN during childhood.
authors.affiliation: Department of Pediatric Nephrology, School of Medicine, Tokyo Women's Medical University, 8-1 Kawada-cho, Shinjuku-ku, Tokyo, 162-8666, Japan.
authors.forename: Shoichiro
authors.initials: S
authors.lastname: Kanda
authors.affiliation: Department of Pathology, Kidney Center, School of Medicine, Tokyo Women's Medical University, Tokyo, Japan.
authors.forename: Shigeru
authors.initials: S
authors.lastname: Horita
authors.affiliation: Department of Pediatrics, Nippon Medical School, Musashikosugi Hospital, Kawasaki, Japan.
authors.forename: Takeshi
authors.initials: T
authors.lastname: Yanagihara
authors.affiliation: Department of Analytic Human Pathology, Nippon Medical School, Tokyo, Japan.
authors.forename: Akira
authors.initials: A
authors.lastname: Shimizu
authors.affiliation: Department of Pediatric Nephrology, School of Medicine, Tokyo Women's Medical University, 8-1 Kawada-cho, Shinjuku-ku, Tokyo, 162-8666, Japan. hattori@kc.twmu.ac.jp.
authors.forename: Motoshi
authors.initials: M
authors.lastname: Hattori
mesh_terms: D000293:Adolescent; D000328:Adult; D000375:Aging / Q000473:pathology; D015415:Biomarkers; D001706:Biopsy; D002648:Child; D002675:Child, Preschool; D015331:Cohort Studies; D005260:Female; D005455:Fluorescent Antibody Technique; D015433:Glomerulonephritis, Membranous / Q000378:metabolism* / Q000473:pathology*; D006801:Humans; D007678:Kidney Glomerulus / Q000378:metabolism* / Q000473:pathology*; D008297:Male; D054507:Receptors, Phospholipase A2
chemical_list: D015415:Biomarkers; C516360:PLA2R1 protein, human; D054507:Receptors, Phospholipase A2
keywords: Biopsy; Idiopathic membranous nephropathy; PLA2R glomerular staining; Pediatric