title: Use of hydrogen peroxide vapour & plasma irradiation in combination for quick decontamination of closed chambers.
abstract: OBJECTIVE
  Various conventional methods such as gaseous, vapour and misting systems, fogging, manual spray and wipe techniques employing a number of chemical agents are used for decontamination of enclosed spaces. Among all these methods, use of aerosolized formaldehyde is the most preferred method due to cost-effectiveness and practical aspects. However, being extremely corrosive in nature generating very irritating fumes and difficulty in maintaining a high level of gas concentration, many laboratories prefer the vaporization of hydrogen peroxide (H 2 O 2 ) as an alternative. We present here the results of using H 2 O 2 vapour in combination with plasma irradiation for quick decontamination of closed chambers.
  
  
  METHODS
  The present study describes a decontamination method, using plasma irradiation in combination with H 2 O 2 (5%). Effect of plasma irradiation and H 2 O 2 on the viability of bacterial spores (Bacillus subtilis), Chikungunya and Kyasanur Forest Disease viruses was assessed.
  
  
  RESULTS
  Data suggest that with the combination of H 2 O 2 vapour and plasma irradiation, within short time (three minutes), decontamination of surfaces and space volume could be achieved. Although it showed damage of spores present on the strips, it did not show any penetration power.
  
  
  CONCLUSIONS
  The results were encouraging, and this method was found to be efficient for achieving surface sterilization in a short time. This application may be useful in laboratories and industries particularly, those working on clean facility concept following good laboratory and manufacturing practices.
authors.affiliation: Maximum Containment Laboratory, Microbial Containment Complex, National Institute of Virology, Pune, India.
authors.forename: Devendra T
authors.initials: DT
authors.lastname: Mourya
authors.affiliation: Klenzaids Contamination Controls (P) Ltd. MIDC, Mumbai, India.
authors.forename: Hamish C
authors.initials: HC
authors.lastname: Shahani
authors.affiliation: Maximum Containment Laboratory, Microbial Containment Complex, National Institute of Virology, Pune, India.
authors.forename: Pragya D
authors.initials: PD
authors.lastname: Yadav
authors.affiliation: Department of Virology, National Institute for Research in Tribal Health, Jabalpur, India.
authors.forename: Pradip V
authors.initials: PV
authors.lastname: Barde
mesh_terms: D001412:Bacillus subtilis / Q000187:drug effects / Q000254:growth & development; D065632:Chikungunya Fever / Q000517:prevention & control / Q000821:virology; D002646:Chikungunya virus / Q000187:drug effects / Q000254:growth & development / Q000528:radiation effects; D003666:Decontamination / Q000379:methods*; D004669:Encephalitis Viruses, Tick-Borne / Q000187:drug effects / Q000528:radiation effects; D004866:Equipment Contamination / Q000517:prevention & control*; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D007733:Kyasanur Forest Disease / Q000517:prevention & control / Q000821:virology; D058626:Plasma Gases / Q000494:pharmacology*; D013171:Spores, Bacterial / Q000187:drug effects / Q000254:growth & development / Q000528:radiation effects; D013242:Sterilization; D014835:Volatilization
chemical_list: D058626:Plasma Gases; D006861:Hydrogen Peroxide