title: A high-throughput screening method of bisphenols, bisphenols digycidyl ethers and their derivatives in dairy products by ultra-high performance liquid chromatography-tandem mass spectrometry.
abstract: A simple and universal analytical method based on ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) for high throughput screening of 21 bisphenols, bisphenols digycidyl ethers and their derivatives in dairy products was developed. Response Surface Methodology (RSM) was used to optimize sample preparation conditions based on a quick, easy, cheap, effective, rugged and safe (QuEChERS) method. The analytes were extracted by using 15 mL acetonitrile with 1% acetic acid, and the extracts were further purified by using 190 mg of C18 and 390 mg of PSA. The extracts were analyzed by UHPLC-MS/MS with electrospray ionization (ESI) source. Linearity was assessed by using matrix-matched standard calibration and good correlation coefficients (r2> 0.99) were obtained. The limits of quantitation (LOQs) for the analytes ranged from 0.02 to 5 μg kg-1. The extraction recoveries were in a range of 88.2%-108.2%. Good method reproducibility in terms of intra- and inter-day precision was observed, yielding relative standard deviations (RSDs) less than 8.9% and 9.9%, respectively. The validation method results revealed that the proposed method was sensitive and reliable. Finally, this method was successfully applied to dairy product analysis.
authors.affiliation: Institute of Food Safety, Chinese Academy of Inspection & Quarantine, Beijing 100176, China; School of Pharmacy, East China University of Science and Technology, Shanghai 200237, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Cheng
authors.affiliation: Institute of Food Safety, Chinese Academy of Inspection & Quarantine, Beijing 100176, China.
authors.forename: Xue-Mei
authors.initials: XM
authors.lastname: Nie
authors.affiliation: Institute of Food Safety, Chinese Academy of Inspection & Quarantine, Beijing 100176, China.
authors.forename: Han-Qiu
authors.initials: HQ
authors.lastname: Wu
authors.affiliation: Institute of Food Safety, Chinese Academy of Inspection & Quarantine, Beijing 100176, China.
authors.forename: Yun-He
authors.initials: YH
authors.lastname: Hong
authors.affiliation: School of Pharmacy, East China University of Science and Technology, Shanghai 200237, China.
authors.forename: Bing-Cheng
authors.initials: BC
authors.lastname: Yang
authors.affiliation: Institute of Food Safety, Chinese Academy of Inspection & Quarantine, Beijing 100176, China.
authors.forename: Tong
authors.initials: T
authors.lastname: Liu
authors.affiliation: Institute of Food Safety, Chinese Academy of Inspection & Quarantine, Beijing 100176, China.
authors.forename: Dan
authors.initials: D
authors.lastname: Zhao
authors.affiliation: Institute of Food Safety, Chinese Academy of Inspection & Quarantine, Beijing 100176, China.
authors.forename: Jian-Feng
authors.initials: JF
authors.lastname: Wang
authors.affiliation: Institute of Food Safety, Chinese Academy of Inspection & Quarantine, Beijing 100176, China.
authors.forename: Gui-Hong
authors.initials: GH
authors.lastname: Yao
authors.affiliation: Institute of Food Safety, Chinese Academy of Inspection & Quarantine, Beijing 100176, China. Electronic address: fengzhang@126.com.
authors.forename: Feng
authors.initials: F
authors.lastname: Zhang
mesh_terms: D000818:Animals; D002417:Cattle; D002851:Chromatography, High Pressure Liquid; D003611:Dairy Products / Q000032:analysis*; D004987:Ethers / Q000032:analysis*; D057166:High-Throughput Screening Assays; D010636:Phenols / Q000032:analysis*; D015203:Reproducibility of Results; D053719:Tandem Mass Spectrometry
chemical_list: D004987:Ethers; D010636:Phenols
keywords: Bisphenols; Bisphenols digycidyl ethers and their derivatives; High throughput; QuEChERS; Ultra-high performance liquid chromatography-tandem mass spectrometry