title: Adenosine Stimulate Proliferation and Migration in Triple Negative Breast Cancer Cells.
abstract: Emerging evidence suggests that the adenosine (Ado) receptors may play crucial roles in tumor progression. Here, we show that Ado increases proliferation and migration in a triple negative breast cancer model, the MDA-MB 231 cell line. The use of specific agonists and antagonists evidenced that these effects depend on the activation of the A2B receptor, which then triggers an intracellular response mediated by the adenylate cyclase/PKA/cAMP signaling pathway. Ado also increases the expression of NaV1.5 channels, a potential biomarker in breast cancer. Together, these data suggest important roles of the A2B receptors and NaV1.5 channels in the Ado-induced increase in proliferation and migration of the MDA-MB 231 cells.
authors.affiliation: Laboratorio de Neuroendocrinología Molecular, Instituto de Fisiología, Benemérita Universidad Autónoma de Puebla (BUAP), Puebla, México.
authors.forename: Miriam
authors.initials: M
authors.lastname: Fernandez-Gallardo
authors.affiliation: Departmento de Biología Molecular e Histocompatibilidad, Hospital General "Dr. Manuel Gea González", Secretaría de Salud, Ciudad de México, México.
authors.forename: Ricardo
authors.initials: R
authors.lastname: González-Ramírez
authors.affiliation: Facultad de Estudios Superiores Iztacala, Universidad Nacional Autónoma de México (UNAM), Tlalnepantla, México.
authors.forename: Alejandro
authors.initials: A
authors.lastname: Sandoval
authors.affiliation: Departmento de Biología Celular, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional (Cinvestav-IPN), Ciudad de México, México.
authors.forename: Ricardo
authors.identifier: http://orcid.org/0000-0002-5383-7176
authors.initials: R
authors.lastname: Felix
authors.affiliation: Laboratorio de Neuroendocrinología Molecular, Instituto de Fisiología, Benemérita Universidad Autónoma de Puebla (BUAP), Puebla, México.
authors.forename: Eduardo
authors.initials: E
authors.lastname: Monjaraz
mesh_terms: D000241:Adenosine / Q000494:pharmacology*; D000262:Adenylyl Cyclases / Q000235:genetics / Q000378:metabolism; D001943:Breast Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects* / Q000235:genetics; D049109:Cell Proliferation / Q000187:drug effects* / Q000235:genetics; D000242:Cyclic AMP / Q000235:genetics / Q000378:metabolism; D017868:Cyclic AMP-Dependent Protein Kinases / Q000235:genetics / Q000378:metabolism; D005260:Female; D006801:Humans; D062554:NAV1.5 Voltage-Gated Sodium Channel / Q000235:genetics / Q000378:metabolism; D009363:Neoplasm Proteins / Q000235:genetics / Q000378:metabolism; D043704:Receptor, Adenosine A2B / Q000235:genetics / Q000378:metabolism; D015290:Second Messenger Systems / Q000187:drug effects*
chemical_list: D062554:NAV1.5 Voltage-Gated Sodium Channel; D009363:Neoplasm Proteins; D043704:Receptor, Adenosine A2B; C568320:SCN5A protein, human; D000242:Cyclic AMP; D017868:Cyclic AMP-Dependent Protein Kinases; D000262:Adenylyl Cyclases; D000241:Adenosine