title: The anti-hyperplasia of mammary gland effect of protein extract HSS from Tegillarca granosa.
abstract: Tegillarca granosa Linnaeus, possesses various biological functions and has been used a Chinese traditional medicine more than one century, but there is no report about anti-hyperplasia of mammary gland (HMG) activity of drugs from T. granosa. In this study, we investigated the anti-HMG effect of protein extract named HSS from T. granosa. The HMG model of virgin female Sprague Dawley rats was prepared by injecting estrogen in the thigh muscle of the rats and progestogen consecutively. HMG rats were treated with either HSS or positive control drug by i.g. for 35 consecutive days. In order to evaluate anti-HMG activity of HSS, Changes of nipple height and diameter, serum sex hormones levels, organ indexes and pathologic changes of mammary gland were performed. Body weight, food intake, pathomorphology examination of organs (heart, liver, spleen, lung, kidney), hematological and biochemical analysis were performed to evaluate the toxicity of HSS. HSS could significantly reduce nipples height and diameter, increase P concentration of HMG rat serum, spleen and thymus index, decrease uterus index, and has therapeutic effect on rat HMG and no toxicity at 500mg/kg/day. The anti-HMG mechanism of HSS may be related to AP-2α and P53. HSS has protective and therapeutic effects on HMG rats, and may be a promising agent for treating hyperplasia of mammary glands.
authors.affiliation: Shandong Academy of Pharmaceutical Sciences, Ji'nan, Shandong, 250101, China. Electronic address: jiayupingygs@163.com.
authors.forename: Yuping
authors.initials: Y
authors.lastname: Jia
authors.affiliation: Shandong Laboratory Animal Center, Shandong Academy of Medical Science, Ji'nan, Shandong, 250002, China.
authors.forename: Xiu
authors.initials: X
authors.lastname: Liu
authors.affiliation: Shandong Academy of Pharmaceutical Sciences, Ji'nan, Shandong, 250101, China.
authors.forename: Qingwen
authors.initials: Q
authors.lastname: Jia
authors.affiliation: Ji'nan Zoo Management Office, Ji'nan, Shandong, 250031, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.affiliation: Shandong Academy of Pharmaceutical Sciences, Ji'nan, Shandong, 250101, China.
authors.forename: Chao
authors.initials: C
authors.lastname: Sun
authors.affiliation: Shandong Academy of Pharmaceutical Sciences, Ji'nan, Shandong, 250101, China.
authors.forename: Dongdong
authors.initials: D
authors.lastname: Yuan
authors.affiliation: China Pharmaceutical University, Nanjing, Jiangsu, 210009, China.
authors.forename: Haoran
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Shandong Academy of Pharmaceutical Sciences, Ji'nan, Shandong, 250101, China.
authors.forename: Ena
authors.initials: E
authors.lastname: Jiang
authors.affiliation: Shandong Laboratory Animal Center, Shandong Academy of Medical Science, Ji'nan, Shandong, 250002, China. Electronic address: zhoudongshun@163.com.
authors.forename: Dongshun
authors.initials: D
authors.lastname: Zhou
mesh_terms: D000818:Animals; D049872:Bivalvia / Q000737:chemistry*; D001835:Body Weight / Q000187:drug effects; D002457:Cell Extracts / Q000494:pharmacology*; D004967:Estrogens / Q000008:administration & dosage / Q000097:blood / Q000633:toxicity; D005260:Female; D006965:Hyperplasia / Q000139:chemically induced* / Q000188:drug therapy*; D008321:Mammary Glands, Animal / Q000187:drug effects* / Q000473:pathology; D011372:Progestins / Q000008:administration & dosage / Q000097:blood / Q000633:toxicity; D011897:Random Allocation; D051381:Rats; D013154:Spleen / Q000187:drug effects; D013950:Thymus Gland / Q000187:drug effects; D014599:Uterus / Q000187:drug effects
chemical_list: D002457:Cell Extracts; D004967:Estrogens; D011372:Progestins
keywords: Anti-HMG; HSS; Morphology of mammary gland; Toxicity