title: Ultrasound-Targeted Microbubble Destruction for Cardiac Gene Delivery.
abstract: Ultrasound targeted microbubble destruction (UTMD) is a novel technique that is used to deliver a gene or other bioactive substance to organs of living animals in a noninvasive manner. Plasmid DNA binding with cationic liposome into nanoparticles are assembled into the shell of microbubbles, which are circulated by intravenous injection. Intermittent bursts of ultrasound with low frequency and high mechanical index destroys the microbubbles and releases the nanoparticles into targeted organ to transfect local organ cells. Cell-specific promoters can be used to further enhance cell specificity. Here we describe UTMD applied to cardiac gene delivery.
authors.affiliation: Division of Cardiology, Department of Internal Medicine, Baylor Heart and Vascular Institute, Baylor University Medical Center, 621 N. Hall St, Suite H030, Dallas, TX, 75226, USA.
authors.forename: Shuyuan
authors.initials: S
authors.lastname: Chen
authors.affiliation: Division of Cardiology, Department of Internal Medicine, Baylor Heart and Vascular Institute, Baylor University Medical Center, 621 N. Hall St, Suite H030, Dallas, TX, 75226, USA. paulgr@baylorhealth.edu.
authors.forename: Paul A
authors.initials: PA
authors.lastname: Grayburn
mesh_terms: D000818:Animals; D057785:Catheters; D004247:DNA / Q000378:metabolism; D018014:Gene Transfer Techniques; D008026:Ligation; D008055:Lipids / Q000737:chemistry; D008081:Liposomes / Q000737:chemistry; D045423:Microbubbles; D009206:Myocardium / Q000378:metabolism*; D010957:Plasmids / Q000378:metabolism; D051381:Rats; D014465:Ultrasonics / Q000379:methods*
chemical_list: D008055:Lipids; D008081:Liposomes; D004247:DNA
keywords: Gene; Gene delivery; Microbubble; Ultrasound; Ultrasound targeted microbubble destruction