title: Laser Microdissection and Spatiotemporal Pinoresinol-Lariciresinol Reductase Gene Expression Assign the Cell Layer-Specific Accumulation of Secoisolariciresinol Diglucoside in Flaxseed Coats.
abstract: The concentration of secoisolariciresinol diglucoside (SDG) found in flaxseed (Linum usitatissimumL.) is higher than that found in any other plant. It exists in flaxseed coats as an SDG-3-hydroxy-3-methylglutaric acid oligomer complex. A laser microdissection method was applied to harvest material from different cell layers of seed coats of mature and developing flaxseed to detect the cell-layer specific localization of SDG in flaxseed; NMR and HPLC were used to identify and quantify SDG in dissected cell layers after alkaline hydrolysis. The obtained results were further confirmed by a standard molecular method. The promoter of one pinoresinol-lariciresinol reductase gene ofL. usitatissimum(LuPLR1), which is a key gene involved in SDG biosynthesis, was fused to a β-glucuronidase (GUS) reporter gene, and the spatio-temporal regulation ofLuPLR1gene expression in flaxseed was determined by histochemical and activity assays ofGUS. The result showed that SDG was synthesized and accumulated in the parenchymatous cell layer of the outer integument of flaxseed coats.
authors.affiliation: Max Planck Institute for Chemical Ecology Jena, Germany.
authors.forename: Jingjing
authors.initials: J
authors.lastname: Fang
authors.affiliation: EA3900 - BioPI Faculté de Pharmacie, Université de Picardie Jules Verne Amiens, France.
authors.forename: Aïna
authors.initials: A
authors.lastname: Ramsay
authors.affiliation: Laboratoire de Biologie des Ligneux et des Grandes Cultures, UPRES EA 1207, Antenne Scientifique Universitaire de Chartres, Université d'Orléans Chartres, France.
authors.forename: Sullivan
authors.initials: S
authors.lastname: Renouard
authors.affiliation: Laboratoire de Biologie des Ligneux et des Grandes Cultures, UPRES EA 1207, Antenne Scientifique Universitaire de Chartres, Université d'Orléans Chartres, France.
authors.forename: Christophe
authors.initials: C
authors.lastname: Hano
authors.affiliation: Laboratoire de Biologie des Ligneux et des Grandes Cultures, UPRES EA 1207, Antenne Scientifique Universitaire de Chartres, Université d'Orléans Chartres, France.
authors.forename: Frédéric
authors.initials: F
authors.lastname: Lamblin
authors.affiliation: INRA, UMR614 Fractionnement des AgroRessources et EnvironnementReims, France; UMR614 Fractionnement des AgroRessources et Environnement, Université de Reims Champagne-ArdenneReims, France.
authors.forename: Brigitte
authors.initials: B
authors.lastname: Chabbert
authors.affiliation: EA3900 - BioPI Faculté de Pharmacie, Université de Picardie Jules Verne Amiens, France.
authors.forename: François
authors.initials: F
authors.lastname: Mesnard
authors.affiliation: Max Planck Institute for Chemical Ecology Jena, Germany.
authors.forename: Bernd
authors.initials: B
authors.lastname: Schneider
keywords: Linum usitatissimum; flaxseed; gene expression; laser microdissection; lignans; localization; pinoresinol-lariciresinol reductase; secoisolariciresinol diglucoside