title: Comparative sequence analysis of enteroaggregative Escherichia coli heat-stable enterotoxin 1 identified in Korean and Japanese Escherichia coli strains.
abstract: The aim of this study was to compare the sequence of the astA gene found in 8 Korean and 11 Japanese Escherichia coli isolates. Conventional PCR was used to amplify the astA gene from the chromosomal and plasmid DNA preparation samples of each isolate using commercial DNA extraction kits. Cloning of the PCR products, sequence analysis, and pulse field gel electrophoresis (PFGE) were sequentially performed. An identical copy of astA in each isolate were found for 8 Korean and 8 Japanese E. coli strains isolated from bovine, porcine, and healthy human carriers. Among these, 1 Korean and 4 Japanese isolates carried a stop mutation at residue 16. Three Japanese outbreak strains (V199, V638, and 96-127-23) carried multiple clones of astA gene with multiple amino acids changes at residues 11, 16, 20, 23, 30, 33, and 34. Compared with the non-diarrheal isolates, clonal diversity and sequence variations of the astA gene in outbreak isolates may be associated with virulence potential of EAST1.
authors.affiliation: Department of Food and Nutrition, Chung-Ang University, 4726 Seodongdaero, Daedeok-myeon, Anseong-si, Gyeonggi-do 17546, Republic of Korea.
authors.forename: Dong Joo
authors.initials: DJ
authors.lastname: Seo
authors.affiliation: Department of Food and Nutrition, Chung-Ang University, 4726 Seodongdaero, Daedeok-myeon, Anseong-si, Gyeonggi-do 17546, Republic of Korea.
authors.forename: SunKeum
authors.initials: S
authors.lastname: Choi
authors.affiliation: Department of Food and Nutrition, Chung-Ang University, 4726 Seodongdaero, Daedeok-myeon, Anseong-si, Gyeonggi-do 17546, Republic of Korea.
authors.forename: Su Been
authors.initials: SB
authors.lastname: Jeon
authors.affiliation: Department of Food and Nutrition, Chung-Ang University, 4726 Seodongdaero, Daedeok-myeon, Anseong-si, Gyeonggi-do 17546, Republic of Korea.
authors.forename: Suntak
authors.initials: S
authors.lastname: Jeong
authors.affiliation: Department of Food and Nutrition, Chung-Ang University, 4726 Seodongdaero, Daedeok-myeon, Anseong-si, Gyeonggi-do 17546, Republic of Korea.
authors.forename: Hyunkyung
authors.initials: H
authors.lastname: Park
authors.affiliation: Department of Food and Nutrition, Chung-Ang University, 4726 Seodongdaero, Daedeok-myeon, Anseong-si, Gyeonggi-do 17546, Republic of Korea.
authors.forename: Bog-Hieu
authors.initials: BH
authors.lastname: Lee
authors.affiliation: School of Bioresources and Bioscience, Chung-Ang University, 4726 Seodongdaero, Daedeok-myeon, Anseong-si, Gyeonggi-do 17546, Republic of Korea.
authors.forename: Geun-Bae
authors.initials: GB
authors.lastname: Kim
authors.affiliation: School of Bioresources and Bioscience, Chung-Ang University, 4726 Seodongdaero, Daedeok-myeon, Anseong-si, Gyeonggi-do 17546, Republic of Korea.
authors.forename: Soo-Jin
authors.initials: SJ
authors.lastname: Yang
authors.affiliation: Department of Food and Human Health Sciences, Graduate School of Human Life Science, Osaka City University, 3-3-138 Sugimoto, Sumiyoshi-ku, Osaka 558-8585, Japan.
authors.forename: Yoshikazu
authors.initials: Y
authors.lastname: Nishikawa
authors.affiliation: Department of Food and Nutrition, Chung-Ang University, 4726 Seodongdaero, Daedeok-myeon, Anseong-si, Gyeonggi-do 17546, Republic of Korea. Electronic address: cchoi@cau.ac.kr.
authors.forename: Changsun
authors.initials: C
authors.lastname: Choi
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002417:Cattle; D016521:Electrophoresis, Gel, Pulsed-Field; D054307:Enterotoxigenic Escherichia coli / Q000235:genetics* / Q000302:isolation & purification* / Q000472:pathogenicity; D004768:Enterotoxins / Q000235:genetics*; D004927:Escherichia coli Infections / Q000382:microbiology; D029968:Escherichia coli Proteins / Q000235:genetics*; D005243:Feces / Q000382:microbiology; D006358:Hot Temperature; D006801:Humans; D007564:Japan; D008460:Meat / Q000382:microbiology; D010957:Plasmids; D016133:Polymerase Chain Reaction; D056910:Republic of Korea; D017422:Sequence Analysis, DNA; D013552:Swine / Q000382:microbiology; D013553:Swine Diseases / Q000382:microbiology; D014774:Virulence / Q000235:genetics
chemical_list: D004768:Enterotoxins; D029968:Escherichia coli Proteins
keywords: Enteroaggregative Escherichia coli heat-stable enterotoxin 1 (EAST1); Escherichia coli; Pulse field gel-electrophoresis (PFGE); Sequencing; astA