title: Cell studies of hybridized carbon nanofibers containing bioactive glass nanoparticles using bone mesenchymal stromal cells.
abstract: Bone regeneration required suitable scaffolding materials to support the proliferation and osteogenic differentiation of bone-related cells. In this study, a kind of hybridized nanofibrous scaffold material (CNF/BG) was prepared by incorporating bioactive glass (BG) nanoparticles into carbon nanofibers (CNF) via the combination of BG sol-gel and polyacrylonitrile (PAN) electrospinning, followed by carbonization. Three types (49 s, 68 s and 86 s) of BG nanoparticles were incorporated. To understand the mechanism of CNF/BG hybrids exerting osteogenic effects, bone marrow mesenchymal stromal cells (BMSCs) were cultured directly on these hybrids (contact culture) or cultured in transwell chambers in the presence of these materials (non-contact culture). The contributions of ion release and contact effect on cell proliferation and osteogenic differentiation were able to be correlated. It was found that the ionic dissolution products had limited effect on cell proliferation, while they were able to enhance osteogenic differentiation of BMSCs in comparison with pure CNF. Differently, the proliferation and osteogenic differentiation were both significantly promoted in the contact culture. In both cases, CNF/BG(68 s) showed the strongest ability in influencing cell behaviors due to its fastest release rate of soluble silicium-relating ions. The synergistic effect of CNF and BG would make CNF/BG hybrids promising substrates for bone repairing.
authors.affiliation: State Key Laboratory of Organic-Inorganic Composites; Beijing Laboratory of Biomedical Materials; Beijing University of Chemical Technology, Beijing 100029, P. R. China.
authors.forename: Xiu-Rui
authors.initials: XR
authors.lastname: Zhang
authors.affiliation: Institute of Sports Medicine, Beijing Key Laboratory of Sports Injury, Peking University Third Hospital, Beijing 100191, P. R. China.
authors.forename: Xiao-Qing
authors.initials: XQ
authors.lastname: Hu
authors.affiliation: State Key Laboratory of Organic-Inorganic Composites; Beijing Laboratory of Biomedical Materials; Beijing University of Chemical Technology, Beijing 100029, P. R. China.
authors.forename: Xiao-Long
authors.initials: XL
authors.lastname: Jia
authors.affiliation: State Key Laboratory of Organic-Inorganic Composites; Beijing Laboratory of Biomedical Materials; Beijing University of Chemical Technology, Beijing 100029, P. R. China.
authors.forename: Li-Ka
authors.initials: LK
authors.lastname: Yang
authors.affiliation: Institute of Sports Medicine, Beijing Key Laboratory of Sports Injury, Peking University Third Hospital, Beijing 100191, P. R. China.
authors.forename: Qing-Yang
authors.initials: QY
authors.lastname: Meng
authors.affiliation: Institute of Sports Medicine, Beijing Key Laboratory of Sports Injury, Peking University Third Hospital, Beijing 100191, P. R. China.
authors.forename: Yuan-Yuan
authors.initials: YY
authors.lastname: Shi
authors.affiliation: Institute of Sports Medicine, Beijing Key Laboratory of Sports Injury, Peking University Third Hospital, Beijing 100191, P. R. China.
authors.forename: Zheng-Zheng
authors.initials: ZZ
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Organic-Inorganic Composites; Beijing Laboratory of Biomedical Materials; Beijing University of Chemical Technology, Beijing 100029, P. R. China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Cai
authors.affiliation: Institute of Sports Medicine, Beijing Key Laboratory of Sports Injury, Peking University Third Hospital, Beijing 100191, P. R. China.
authors.forename: Yin-Fang
authors.initials: YF
authors.lastname: Ao
authors.affiliation: State Key Laboratory of Organic-Inorganic Composites; Beijing Laboratory of Biomedical Materials; Beijing University of Chemical Technology, Beijing 100029, P. R. China.
authors.forename: Xiao-Ping
authors.initials: XP
authors.lastname: Yang
mesh_terms: D000818:Animals; D019485:Bone Morphogenetic Proteins / Q000378:metabolism; D001861:Bone Regeneration; D002244:Carbon / Q000737:chemistry; D002454:Cell Differentiation; D002470:Cell Survival; D020869:Gene Expression Profiling; D005898:Glass / Q000737:chemistry; D008422:Materials Testing; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism; D057139:Nanofibers / Q000737:chemistry / Q000648:ultrastructure; D053758:Nanoparticles / Q000737:chemistry / Q000648:ultrastructure; D010012:Osteogenesis; D051381:Rats; D054457:Tissue Scaffolds
chemical_list: D019485:Bone Morphogenetic Proteins; D002244:Carbon