title: Early responses of human periodontal ligament fibroblasts to cyclic and static mechanical stretching.
abstract: To compare the mechanotransduction caused by cyclic and static mechanical strains in human periodontal ligament fibroblasts (hPDLFs) cultured under identical conditions.
  
  
  
  hPDLFs, originating from the same donors, were exposed either to cyclic or to static tensile strain using specially designed devices and under identical culture conditions. Activation of all members of mitogen-activated protein kinases (MAPKs) was monitored by western immunoblot analysis. Expression levels of immediate/early genes c-fos and c-jun were assessed with quantitative real-time polymerase chain reaction.
  
  
  
  Time course experiments revealed that both types of stresses activate the three members of MAPK, that is ERK, p38, and JNK, with cyclic stress exhibiting a slightly more extended activation. Further downstream, both stresses upregulate the immediate/early genes c-fos and c-jun, encoding components of the activator protein-1 (AP-1), a key transcription factor in osteoblastic differentiation; again cyclic strain provokes a more intense upregulation. Six hours after the application of both strains, MAPK activation and gene expression return to basal levels. Finally, cells exposed to cyclic stress for longer periods are distributed approximately perpendicular to the axis of the applied strain, whereas cells exposed to static loading remain in a random orientation in culture.
  
  
  
  The findings of the present study indicate similar, although not identical, immediate/early responses of hPDLs to cyclic and static stretching, with cyclic strain provoking a more intense adaptive response of these cells to mechanical deformation.
authors.affiliation: Laboratory of Cell Proliferation and Ageing, Institute of Biosciences and Applications, National Centre for Scientific Research 'Demokritos', Athens.
authors.forename: Adamantia
authors.initials: A
authors.lastname: Papadopoulou
authors.affiliation: Department of Orthodontics, School of Dentistry, University of Athens, Greece.
authors.forename: Anna
authors.initials: A
authors.lastname: Iliadi
authors.affiliation: Clinic of Orthodontics and Paediatric Dentistry, University of Zurich, Switzerland.
authors.forename: Theodore
authors.initials: T
authors.lastname: Eliades
authors.affiliation: Laboratory of Cell Proliferation and Ageing, Institute of Biosciences and Applications, National Centre for Scientific Research 'Demokritos', Athens.
authors.forename: Dimitris
authors.initials: D
authors.lastname: Kletsas
mesh_terms: D000293:Adolescent; D002454:Cell Differentiation / Q000502:physiology; D002478:Cells, Cultured; D002648:Child; D005347:Fibroblasts / Q000502:physiology*; D005786:Gene Expression Regulation / Q000502:physiology; D006801:Humans; D048031:JNK Mitogen-Activated Protein Kinases / Q000378:metabolism; D020935:MAP Kinase Signaling System / Q000502:physiology; D040542:Mechanotransduction, Cellular / Q000502:physiology*; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism; D010513:Periodontal Ligament / Q000166:cytology* / Q000378:metabolism; D013314:Stress, Mechanical; D018808:Transcription Factor AP-1 / Q000378:metabolism; D055815:Young Adult
chemical_list: D018808:Transcription Factor AP-1; D048031:JNK Mitogen-Activated Protein Kinases; D020928:Mitogen-Activated Protein Kinases