title: Mycophenolate mofetil attenuates uterine ischaemia/reperfusion injury in a rat model.
abstract: This study evaluated the effect of mycophenolate mofetil (MMF) on uterine tissue preservation following ischaemia/reperfusion (I/R) injury. Uterine I/R injury was induced in rats by clamping the lower abdominal aorta and ovarian arteries for 30 min. Group I/R + V (n = 7) received vehicle alone while Group I/R + M (n = 7) received 20 mg/kg/day MMF. Control groups underwent sham surgery and received vehicle (Group C) or 20 mg/kg/day MMF (Group M) (n = 7 for both). Four hours after detorsion, uterine tissue 8-hydroxy-2'-deoxyguanosine (8-OHdG), glutathione, malondialdehyde (MDA), myeloperoxidase (MPO), superoxide dismutase (SOD) and serum ischaemia modified albumin (IMA) concentrations were measured. Histopathological analyses were performed. The I/R + M group showed significant reduction in serum IMA and uterine tissue 8-OHdG, MDA and MPO and significant increase in SOD concentrations compared with the I/R + V group, indicating a protective effect against I/R oxidative damage (P = 0.009, P = 0.006, P = 0.002, P = 0.003 and P = 0.009, respectively). Histopathological evaluation revealed MMF treatment resulted in significantly less tissue and cellular damage and apoptosis compared with the I/R + V group. These results indicate MMF is effective in attenuating uterine tissue damage and preventing apoptosis following uterine I/R injury, probably via anti-inflammatory and anti-oxidative action.
authors.affiliation: Department of Obstetrics and Gynecology, Kartal Dr Lutfi Kirdar Education and Research Hospital, Istanbul, Turkey. Electronic address: gulcinsahinmd@gmail.com.
authors.forename: Gulcin
authors.initials: G
authors.lastname: Sahin Ersoy
authors.affiliation: Department of Obstetrics and Gynecology, Zeynep Kamil Education and Research Hospital, Istanbul, Turkey.
authors.forename: Meryem
authors.initials: M
authors.lastname: Kurek Eken
authors.affiliation: Department of Biochemistry, Faculty of Pharmacy, Cumhuriyet University, Sivas, Turkey.
authors.forename: Ozge
authors.initials: O
authors.lastname: Cevik
authors.affiliation: Department of Histology and Embryology, Marmara University School of Medicine, Istanbul, Turkey.
authors.forename: Ozlem T
authors.initials: OT
authors.lastname: Cilingir
authors.affiliation: Division of Reproductive Endocrinology and Infertility, Department of Obstetrics, Gynecology and Reproductive Sciences, Yale University School of Medicine, New Haven, CT, USA.
authors.forename: Reshef
authors.initials: R
authors.lastname: Tal
mesh_terms: D000080242:8-Hydroxy-2'-Deoxyguanosine; D000418:Albumins / Q000378:metabolism; D000818:Animals; D000903:Antibiotics, Antineoplastic / Q000494:pharmacology*; D000975:Antioxidants / Q000378:metabolism; D001012:Aorta, Abdominal / Q000473:pathology; D001158:Arteries / Q000473:pathology; D003849:Deoxyguanosine / Q000031:analogs & derivatives / Q000378:metabolism; D004195:Disease Models, Animal; D005260:Female; D005978:Glutathione / Q000378:metabolism; D007166:Immunosuppressive Agents / Q000627:therapeutic use; D009173:Mycophenolic Acid / Q000494:pharmacology*; D010053:Ovary / Q000098:blood supply; D009195:Peroxidase / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000188:drug therapy* / Q000473:pathology; D013482:Superoxide Dismutase / Q000378:metabolism; D014599:Uterus / Q000473:pathology*
chemical_list: D000418:Albumins; D000903:Antibiotics, Antineoplastic; D000975:Antioxidants; D007166:Immunosuppressive Agents; D000080242:8-Hydroxy-2'-Deoxyguanosine; D009195:Peroxidase; D013482:Superoxide Dismutase; D003849:Deoxyguanosine; D005978:Glutathione; D009173:Mycophenolic Acid
keywords: mycophenolate mofetil; oxygen species-free radicals; rat; uterine ischaemia-reperfusion; uterine transplantation