title: Micro RNA-550a interferes with vitamin D metabolism in peripheral B cells of patients with diabetes.
abstract: The pathogenesis of diabetes is to be further investigated. Vitamin D3 (VitD3) can improve diabetes. Micro RNAs (miR) are involved in regulating cell activities. This study tests a hypothesis that miR-550a interferes with the metabolism of VitD3 in peripheral B cells. In this study, blood samples were collected from patients with diabetes and healthy persons. The B cells were isolated from the blood samples to be treated with tumor necrosis factor (TNF)-α. The B cells were then collected and analyzed for the expression of miR-550a and cyp27b1. The results showed that B cells from healthy subjects were capable of converting VitD metabolite calcidiol to calcitriol, which was impaired in B cells collected from diabetic patients. The diabetic patients showed lower bone mineral density than that in healthy subject. The miR-550a was negatively correlated with bone mineral density and the Levels of cyp27b1 in peripheral B cells of patients with diabetes. In vitro study showed that TNF-α increased miR-550a expression and inhibited the expression of cyp27b1 in B cells. miR-550a mediated the effects of TNF-α on inducing chromatin remodeling at the cyp27b1 gene locus. In conclusion, miR-550a mediates the TNF-α-induced suppression of cyp27b1 expression in peripheral B cells of patients with diabetes, which can be blocked by inhibition of miR-550a.
authors.affiliation: Department of Cadre Clinic, Chinese PLA General Hospital, Beijing, 100853, China.
authors.forename: Jinggui
authors.initials: J
authors.lastname: He
authors.affiliation: Department of Cadre Clinic, Chinese PLA General Hospital, Beijing, 100853, China.
authors.forename: Xiyun
authors.initials: X
authors.lastname: Guo
authors.affiliation: The Research Center of Allergy & Immunology, Shenzhen University School of Medicine, Shenzhen, 518060, China.
authors.forename: Zhi-Qiang
authors.initials: ZQ
authors.lastname: Liu
authors.affiliation: The Research Center of Allergy & Immunology, Shenzhen University School of Medicine, Shenzhen, 518060, China.
authors.forename: Ping-Chang
authors.initials: PC
authors.lastname: Yang
authors.affiliation: Department of Cadre Clinic, Chinese PLA General Hospital, Beijing, 100853, China.
authors.forename: Shaobo
authors.initials: S
authors.lastname: Yang
mesh_terms: D015090:25-Hydroxyvitamin D3 1-alpha-Hydroxylase / Q000235:genetics / Q000378:metabolism; D000328:Adult; D001402:B-Lymphocytes / Q000187:drug effects / Q000378:metabolism*; D015519:Bone Density / Q000187:drug effects; D002112:Calcifediol / Q000378:metabolism; D002117:Calcitriol / Q000378:metabolism; D042002:Chromatin Assembly and Disassembly / Q000187:drug effects; D003920:Diabetes Mellitus / Q000378:metabolism*; D051766:Early Growth Response Protein 1 / Q000378:metabolism; D005260:Female; D056426:Genetic Loci; D056284:Histone Deacetylase 1 / Q000378:metabolism; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology; D015854:Up-Regulation / Q000187:drug effects; D014807:Vitamin D / Q000378:metabolism*
chemical_list: C497325:EGR1 protein, human; D051766:Early Growth Response Protein 1; C000613721:MIRN550 microRNA, human; D035683:MicroRNAs; D014409:Tumor Necrosis Factor-alpha; D014807:Vitamin D; D015090:25-Hydroxyvitamin D3 1-alpha-Hydroxylase; C000600378:CYP27B1 protein, human; C110550:HDAC1 protein, human; D056284:Histone Deacetylase 1; D002117:Calcitriol; D002112:Calcifediol
keywords: B cells; Cyp27b1; TNF-α; Vitamin D3; micro RNA