title: [The regulatory role of Wnt signaling pathway in the proliferation and activation of memory T cells].
abstract: Objective To study the regulatory effect of Wnt signaling pathway on the activation and proliferation of memory T cells (TMs). Methods We established the mouse model of skin allograft transplantation (BALB/c-C57BL/6), and then we sorted and cultured immature dendritic cells (imDCs), mature dendritic cells (mDCs) and TMs from the C57BL/6 mice by immunomagnetic bead cell sorting. The imDCs or mDCs loading antigens from BALB/c mice were co-cultured with TMs in a 96-well plate or TranswellTMplate (MLR). The3H-TdR was used to detect the proliferation of TMs. In the co-culture supernatants, IL-2, IL-10 and IFN- were detected by ELISA. The Wnt mRNA expression levels in imDCs and mDCs were determined by real-time PCR. Dual-luciferase reporter assay was performed to detect the transcriptional activity of β-catenin/TCF signaling in TMs. Results Compared with the control group, mDCs loading antigens from BALB/c mice induced remarkably the proliferation of TMs, increased the expressions of IL-2 and IFN-γ, and decreased the expression of IL-10. The expression levels of Wnt3a and Wnt5a were significantly higher in mDCs than in imDCs. The transcriptional activity of β-catenin/TCF signaling pathway in TMs was obviously enhanced after co-cultured with mDCs. Conclusion The mDCs loading donor-specific antigens from the recipient mice could induce the proliferation of effector TMs of recipients through direct contact, and Wnt signaling pathway mediated β-catenin/TCF transcriptional activation may be one of the important signaling pathways to induce the activation and proliferation of TMs.
authors.affiliation: Evidence-based Medicine Section, Department of Research and Education, Guizhou Province People's Hospital, Guiyang 550002, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Luo
authors.affiliation: Evidence-based Medicine Section, Department of Research and Education, Guizhou Province People's Hospital, Guiyang 550002, China.
authors.forename: Guangheng
authors.initials: G
authors.lastname: Luo
authors.affiliation: Evidence-based Medicine Section, Department of Research and Education, Guizhou Province People's Hospital, Guiyang 550002, China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Fang
authors.affiliation: Evidence-based Medicine Section, Department of Research and Education, Guizhou Province People's Hospital, Guiyang 550002, China.
authors.forename: Shan
authors.initials: S
authors.lastname: Huang
authors.affiliation: Evidence-based Medicine Section, Department of Research and Education, Guizhou Province People's Hospital, Guiyang 550002, China. *Corresponding author, E-mail: huichen7710@hotmail.com.
authors.forename: Hui
authors.initials: H
authors.lastname: Chen
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000502:physiology; D049109:Cell Proliferation / Q000235:genetics / Q000502:physiology*; D003713:Dendritic Cells / Q000276:immunology / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay; D016753:Interleukin-10 / Q000378:metabolism; D007376:Interleukin-2 / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D060888:Real-Time Polymerase Chain Reaction; D013601:T-Lymphocytes / Q000276:immunology / Q000378:metabolism*; D060449:Wnt Signaling Pathway / Q000235:genetics / Q000502:physiology*; D051176:beta Catenin / Q000378:metabolism
chemical_list: D007376:Interleukin-2; D051176:beta Catenin; D016753:Interleukin-10