title: Old Apple (Malus domestica L. Borkh) Varieties with Hypoallergenic Properties: An Integrated Approach for Studying Apple Allergenicity.
abstract: Freshly consumed apples (Malus domestica L. Borkh) can cause allergic reactions because of the presence of four classes of allergens. Knowledge of the genetic factors affecting the allergenic potential of apples would provide important information for the selection of hypoallergenic genotypes, which can be combined with the adoption of new agronomical practices to produce fruits with a reduced amount of allergens. In the present research, a multiple analytical approach was adopted to characterize the allergenic potential of 24 apple varieties released at different ages (pre- and post-green revolution). A specific workflow was set up including protein quantification by means of polyclonal antibodies, immunological analyses with sera of allergic subjects, enzymatic assays, clinical assessments on allergic patients, and gene expression assays on fruit samples. Taken as a whole, the results indicate that most of the less allergenic genotypes were found among those deriving from selection processes carried out prior to the so-called "green revolution".
authors.affiliation: Department of Agronomy, Food, Natural Resources, Animals and Environment - DAFNAE - Agripolis, University of Padova , Viale dell'università 16, 35020 Legnaro (Padova), Italy.
authors.forename: Mara
authors.initials: M
authors.lastname: Vegro
authors.affiliation: Department of Agronomy, Food, Natural Resources, Animals and Environment - DAFNAE - Agripolis, University of Padova , Viale dell'università 16, 35020 Legnaro (Padova), Italy.
authors.forename: Giulia
authors.initials: G
authors.lastname: Eccher
authors.affiliation: Department of Agronomy, Food, Natural Resources, Animals and Environment - DAFNAE - Agripolis, University of Padova , Viale dell'università 16, 35020 Legnaro (Padova), Italy.
authors.forename: Francesca
authors.initials: F
authors.lastname: Populin
authors.affiliation: Department of Agronomy, Food, Natural Resources, Animals and Environment - DAFNAE - Agripolis, University of Padova , Viale dell'università 16, 35020 Legnaro (Padova), Italy.
authors.forename: Chiara
authors.initials: C
authors.lastname: Sorgato
authors.affiliation: Department of Agricultural Science, University of Bologna , Viale Fanin 46, 40127 Bologna, Italy.
authors.forename: Federica
authors.initials: F
authors.lastname: Savazzini
authors.affiliation: Department of Agricultural Science, University of Bologna , Viale Fanin 46, 40127 Bologna, Italy.
authors.forename: Giulia
authors.initials: G
authors.lastname: Pagliarani
authors.affiliation: Department of Agricultural Science, University of Bologna , Viale Fanin 46, 40127 Bologna, Italy.
authors.forename: Stefano
authors.initials: S
authors.lastname: Tartarini
authors.affiliation: Department of Agronomy, Food, Natural Resources, Animals and Environment - DAFNAE - Agripolis, University of Padova , Viale dell'università 16, 35020 Legnaro (Padova), Italy.
authors.forename: Gabriella
authors.initials: G
authors.lastname: Pasini
authors.affiliation: Department of Agronomy, Food, Natural Resources, Animals and Environment - DAFNAE - Agripolis, University of Padova , Viale dell'università 16, 35020 Legnaro (Padova), Italy.
authors.forename: Andrea
authors.initials: A
authors.lastname: Curioni
authors.affiliation: Allergy Unit, Ospedale Civile Srl , Via Guido Tonello 5, 46049 Volta Mantovana (Mantova), Italy.
authors.forename: Andrea
authors.initials: A
authors.lastname: Antico
authors.affiliation: Department of Agronomy, Food, Natural Resources, Animals and Environment - DAFNAE - Agripolis, University of Padova , Viale dell'università 16, 35020 Legnaro (Padova), Italy.
authors.forename: Alessandro
authors.initials: A
authors.lastname: Botton
mesh_terms: D000485:Allergens / Q000276:immunology*; D052179:Antigens, Plant / Q000276:immunology*; D005512:Food Hypersensitivity / Q000276:immunology*; D005638:Fruit / Q000737:chemistry*; D005838:Genotype; D006801:Humans; D027845:Malus / Q000737:chemistry / Q000235:genetics*; D010940:Plant Proteins / Q000276:immunology*
chemical_list: D000485:Allergens; D052179:Antigens, Plant; D010940:Plant Proteins
keywords: Malus domestica; gene expression studies; polyclonal antibodies; prick-by-prick tests