title: Metabolomic Investigation of Rat Serum Following Oral Administration of the Willow Bracket Medicinal Mushroom, Phellinus igniarius (Agaricomycetes), by UPLC-HDMS.
abstract: The medicinal willow bracket mushroom, Phellinus igniarius, is a species that has been reported to possess antibacterial, antioxidative, antitumor, antidiabetic, and antihyperlipidemia activities. The aim of this study was to elucidate the changes in endogenous metabolites after oral administration of a decoction of Ph. Igniarius. Ultraperformance liquid chromatography (UPLC)/electrospray ionization synapt high-definition mass spectrometry (ESI-HDMS) combined with pattern recognition approaches, including principal component analysis and orthogonal partial least squares discriminant analysis, were integrated to discover differentiating metabolites. The current metabolomics approach identified 16 ions (5 in the negative mode, 11 in the positive mode) as "differentiating metabolites". The results illustrated that Ph. Igniarius is likely to increase the biosynthesis and secretion of bile acids that provide hypolipidemic activity and showed that robust UPLC/ESI-HDMS techniques are promising for profiling analysis of medicinal mushroom metabolites.
authors.affiliation: Department of Medicine, Zhejiang Academy of Traditional Chinese Medicine, Hangzhou, China; Zhejiang Chinese Medical University, Hangzhou, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Dong
authors.affiliation: Zhejiang Chinese Medical University, Hangzhou, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: He
authors.affiliation: Department of Medicine, Zhejiang Academy of Traditional Chinese Medicine, Hangzhou, China.
authors.forename: Zhongming
authors.initials: Z
authors.lastname: Yu
authors.affiliation: Department of Medicine, Zhejiang Academy of Traditional Chinese Medicine, Hangzhou, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Medicine, Zhejiang Academy of Traditional Chinese Medicine, Hangzhou, China.
authors.forename: Nani
authors.initials: N
authors.lastname: Wang
authors.affiliation: Department of Medicine, Zhejiang Academy of Traditional Chinese Medicine, Hangzhou, China; Department of Chemistry, Xixi Campus, Zhejiang University, Hangzhou, China.
authors.forename: Dan
authors.initials: D
authors.lastname: Shou
authors.affiliation: Zhejiang Chinese Medical University, Hangzhou, China.
authors.forename: Changyu
authors.initials: C
authors.lastname: Li
mesh_terms: D000284:Administration, Oral; D000363:Agaricales / Q000737:chemistry*; D000818:Animals; D002853:Chromatography, Liquid / Q000379:methods; D050356:Lipid Metabolism; D008297:Male; D013058:Mass Spectrometry / Q000379:methods; D055442:Metabolome / Q000187:drug effects*; D051381:Rats; D017208:Rats, Wistar