title: cdc-25.2, a Caenorhabditis elegans ortholog of cdc25, is required for male tail morphogenesis.
abstract: Cell division cycle 25 (Cdc25) is an evolutionarily conserved phosphatase that promotes cell cycle progression by activating cyclin-dependent kinases (Cdks) which are inactivated by Wee1/Myt1 kinases. It was previously reported that cdc-25.2 promotes oocyte maturation and intestinal cell divisions in Caenorhabditis elegans hermaphrodites. Here, we report a novel function of cdc-25.2 in male tail development which was significantly deformed by cdc-25.2 RNAi depletion and in cdc-25.2 mutant males. The deformation was also observed after RNAi depletion of other cell cycle regulators, cdk-1, cyb-3, cyd-1, and cyl-1. Furthermore, wee-1.3 counteracted cdc-25.2 in male tail development as observed in oocyte maturation and intestine development. The number of cells in ray precursor cell lineages was significantly reduced in cdc-25.2 depleted males. These results indicate that CDC-25.2 is essential for cell divisions in ray precursor cell lineages for proper male tail development.
authors.affiliation: Department of Bioscience and Biotechnology, Konkuk University, Seoul 05029, Republic of Korea.
authors.forename: Sangmi
authors.initials: S
authors.lastname: Oh
authors.affiliation: Department of Bioscience and Biotechnology, Konkuk University, Seoul 05029, Republic of Korea.
authors.forename: Sunghee
authors.initials: S
authors.lastname: Yoon
authors.affiliation: Department of Bioscience and Biotechnology, Konkuk University, Seoul 05029, Republic of Korea.
authors.forename: Esther
authors.initials: E
authors.lastname: Youn
authors.affiliation: Department of Bioscience and Biotechnology, Konkuk University, Seoul 05029, Republic of Korea.
authors.forename: Ichiro
authors.initials: I
authors.lastname: Kawasaki
authors.affiliation: Department of Bioscience and Biotechnology, Konkuk University, Seoul 05029, Republic of Korea. Electronic address: yshim@konkuk.ac.kr.
authors.forename: Yhong-Hee
authors.initials: YH
authors.lastname: Shim
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D017173:Caenorhabditis elegans / Q000196:embryology*; D029742:Caenorhabditis elegans Proteins / Q000502:physiology*; D002453:Cell Cycle; D002455:Cell Division; D019070:Cell Lineage; D018844:Cyclin-Dependent Kinases / Q000378:metabolism; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D008297:Male; D009024:Morphogenesis; D010641:Phenotype; D010749:Phosphoprotein Phosphatases / Q000502:physiology*; D034622:RNA Interference; D013623:Tail / Q000196:embryology*; D019076:Transgenes
chemical_list: D029742:Caenorhabditis elegans Proteins; D018844:Cyclin-Dependent Kinases; D010749:Phosphoprotein Phosphatases; C550003:cdc-25.2 protein, C elegans
keywords: Caenorhabditis elegans; Cdc25 phosphatase; Cell cycle regulator; Male tail development; Ray precursor cell