title: Gene Transfer to Rodent Hearts In Vivo.
abstract: Altering expression of specific genes in rodent models allows the dissection of various pathways involved in various disease states. Advances in gene transfer technologies produced vectors with tissue specificity, various time courses, increasing or knocking down genes in an externally controlled fashion. To achieve this goal, appropriate vector delivery method is essential for successfully conducting experiments. In this chapter, we focus on cardiac gene transfer in rodents that can be employed for delivering both viral and nonviral vectors.
authors.affiliation: Cardiovascular Research Center, Beth Israel Deaconess Hospital, Boston, MA, USA.
authors.forename: Federica
authors.initials: F
authors.lastname: Del Monte
authors.affiliation: Cardiovascular Research Center, Icahn School of Medicine at Mount Sinai, One Gustave L. Levy Place, Box 1030, New York, NY, 10029-6574, USA.
authors.forename: Kiyotake
authors.initials: K
authors.lastname: Ishikawa
authors.affiliation: Cardiovascular Research Center, Icahn School of Medicine at Mount Sinai, One Gustave L. Levy Place, Box 1030, New York, NY, 10029-6574, USA. roger.hajjar@mssm.edu.
authors.forename: Roger J
authors.initials: RJ
authors.lastname: Hajjar
mesh_terms: D000758:Anesthesia; D000818:Animals; D001011:Aorta / Q000378:metabolism; D005260:Female; D018014:Gene Transfer Techniques; D005822:Genetic Vectors / Q000378:metabolism; D006321:Heart / Q000502:physiology*; D007267:Injections; D008297:Male; D011651:Pulmonary Artery / Q000378:metabolism; D051381:Rats; D014680:Veins / Q000378:metabolism
keywords: Adeno virus; Adeno-associated virus; Gene delivery; Modified RNA; Mouse; Plasmid; Rat; Small animals