title: Circadian transcription factor BMAL1 regulates innate immunity against select RNA viruses.
abstract: BMAL1 (brain and muscle ARNT-like protein 1, also known as MOP3 or ARNT3) belongs to the family of the basic helix-loop-helix (bHLH)-PAS domain-containing transcription factors, and is a key component of the molecular oscillator that generates circadian rhythms. Here, we report that BMAL1-deficient cells are significantly more susceptible to infection by two major respiratory viruses of the Paramyxoviridae family, namely RSV and PIV3. Embryonic fibroblasts from Bmal1-/-mice produced nearly 10-fold more progeny virus than their wild type controls. These results were supported by animal studies whereby pulmonary infection of RSV produced a more severe disease and morbidity in Bmal1-/-mice. These results show that BMAL1 can regulate cellular innate immunity against specific RNA viruses.
authors.affiliation: Department of Biological, Geological and Environmental Sciences, and Center for Gene Regulation in Health and Disease, Cleveland State University, Cleveland, OH, USA.
authors.forename: Tanmay
authors.initials: T
authors.lastname: Majumdar
authors.affiliation: Department of Biological, Geological and Environmental Sciences, and Center for Gene Regulation in Health and Disease, Cleveland State University, Cleveland, OH, USA.
authors.forename: Jayeeta
authors.initials: J
authors.lastname: Dhar
authors.affiliation: Department of Biological, Geological and Environmental Sciences, and Center for Gene Regulation in Health and Disease, Cleveland State University, Cleveland, OH, USA.
authors.forename: Sonal
authors.initials: S
authors.lastname: Patel
authors.affiliation: Department of Biological, Geological and Environmental Sciences, and Center for Gene Regulation in Health and Disease, Cleveland State University, Cleveland, OH, USA.
authors.forename: Roman
authors.initials: R
authors.lastname: Kondratov
authors.affiliation: Department of Biological, Geological and Environmental Sciences, and Center for Gene Regulation in Health and Disease, Cleveland State University, Cleveland, OH, USA.
authors.forename: Sailen
authors.initials: S
authors.lastname: Barik
mesh_terms: D056930:ARNTL Transcription Factors / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D002460:Cell Line; D057906:Circadian Clocks / Q000235:genetics; D005347:Fibroblasts / Q000276:immunology* / Q000821:virology; D006801:Humans; D007113:Immunity, Innate / Q000235:genetics; D008168:Lung / Q000473:pathology*; D051379:Mice; D018345:Mice, Knockout; D010224:Parainfluenza Virus 3, Human / Q000276:immunology*; D034741:RNA, Small Interfering / Q000235:genetics; D018357:Respiratory Syncytial Virus Infections / Q000276:immunology*; D012136:Respiratory Syncytial Viruses / Q000276:immunology*; D010253:Respirovirus Infections / Q000276:immunology*
chemical_list: D056930:ARNTL Transcription Factors; D034741:RNA, Small Interfering
keywords: BMAL1; Paramyxovirus; Toxoplasma gondii; circadian clock; innate immunity; parainfluenza virus; pneumovirus; respiratory syncytial virus