title: Chitosan nanoparticles as a dual drug/siRNA delivery system for treatment of colorectal cancer.
abstract: Nanoparticles are widely used to deliver anticancer drugs and inhibit tumor growth without systemic toxicity. Recently, chitosan has received much attention as a functional biopolymer for encapsulation of small interfering RNA (siRNA). Because of cationic nature, chitosan efficiently encapsulate siRNA and forming nanoparticles. Moreover, biocompatible and biodegradable properties represent chitosan as potential candidate for in vivo siRNA delivery. In the present study we designed carboxymethyl dextran (CMD) chitosan nanoparticles (ChNPs) to encapsulate snail siRNA and anticancer drug doxorubicin (DOX). The effect of ChNPs-drug/siRNA on cell growth and Epithelial mesenchymal transition (EMT) gene expression of HCT-116 cell lines were investigated. Furthermore the efficacy of dual agent nanoparticle to induce apoptosis and inhibit migration of colorectal cancer cells were assessed using Annexin-V and wound healing assays, respectively. The results demonstrated that treatment with dual agent nanoparticles led to significant changes of EMT genes (i.e down regulation of MMP-9 and Vimentin and up regulation of E-cadherin), apoptosis cell death and migration inhibition in HCT-116 cells. In conclusion, ChNPs encapsulating DOX and snail siRNA can be considered as an effective anti-cancer drugs delivery system for the treatment of colorectal cancer.
authors.affiliation: Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran; Department of Animal Biology, Faculty of Natural Sciences, The University of Tabriz, Tabriz, Iran.
authors.forename: Sanam
authors.initials: S
authors.lastname: Sadreddini
authors.affiliation: Department of Animal Biology, Faculty of Natural Sciences, The University of Tabriz, Tabriz, Iran.
authors.forename: Reza
authors.initials: R
authors.lastname: Safaralizadeh
authors.affiliation: Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
authors.forename: Behzad
authors.initials: B
authors.lastname: Baradaran
authors.affiliation: Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
authors.forename: Leili
authors.initials: L
authors.lastname: Aghebati-Maleki
authors.affiliation: Department of Animal Biology, Faculty of Natural Sciences, The University of Tabriz, Tabriz, Iran.
authors.forename: Mohammad Ali
authors.initials: MA
authors.lastname: Hosseinpour-Feizi
authors.affiliation: Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
authors.forename: Dariush
authors.initials: D
authors.lastname: Shanehbandi
authors.affiliation: Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
authors.forename: Farhad
authors.initials: F
authors.lastname: Jadidi-Niaragh
authors.affiliation: Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
authors.forename: Sevil
authors.initials: S
authors.lastname: Sadreddini
authors.affiliation: Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
authors.forename: Hossein Samadi
authors.initials: HS
authors.lastname: Kafil
authors.affiliation: Monoclonal Antibody Research Center, Avicenna Research Institute, ACECR, Tehran, Iran.
authors.forename: Vahid
authors.initials: V
authors.lastname: Younesi
authors.affiliation: Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran; Department of Immunology, School of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran. Electronic address: Yousefime@tbzmed.ac.ir.
authors.forename: Mehdi
authors.initials: M
authors.lastname: Yousefi
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000008:administration & dosage*; D015415:Biomarkers; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D048271:Chitosan; D015179:Colorectal Neoplasms / Q000235:genetics* / Q000473:pathology* / Q000628:therapy; D004195:Disease Models, Animal; D016503:Drug Delivery Systems; D015972:Gene Expression Regulation, Neoplastic; D018014:Gene Transfer Techniques; D006801:Humans; D051379:Mice; D053758:Nanoparticles; D034741:RNA, Small Interfering / Q000008:administration & dosage* / Q000235:genetics*; D017550:Spectroscopy, Fourier Transform Infrared; D014945:Wound Healing / Q000187:drug effects / Q000235:genetics; D023041:Xenograft Model Antitumor Assays
chemical_list: D000970:Antineoplastic Agents; D015415:Biomarkers; D034741:RNA, Small Interfering; D048271:Chitosan
keywords: Chitosan nanoparticle; Colorectal cancer; Doxorubicin; Snail; siRNA