title: A Jacalin-Related Lectin Regulated the Formation of Aerial Mycelium and Fruiting Body in Flammulina velutipes.
abstract: Flammulina velutipes, one of the most popular mushroom species in the world, has been recognized as a useful model system to study the biochemical and physiological aspects of the formation and elongation of fruit body. However, few reports have been published on the regulation of fruiting body formation inF. velutipesat the molecular level. In this study, a jacalin-related lectin gene fromF. velutipeswas characterized. The phylogenetic tree revealed that Fv-JRL1 clustered with other basidiomycete jacalin-like lectins. Moreover, the transcriptional pattern of theFv-JRL1gene in different developmental stages ofF. velutipesimplied thatFv-JRL1could be important for formation of fruit body. Additionally, RNA interference (RNAi) and overexpression analyses provided powerful evidence that the lectin geneFv-JRL1fromF. velutipesplays important roles in fruiting body formation.
authors.affiliation: Mycological Research Center, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China. yuanplu1106@163.com.
authors.forename: Yuan-Ping
authors.initials: YP
authors.lastname: Lu
authors.affiliation: Mycological Research Center, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China. renlchen2306126618@163.com.
authors.forename: Ren-Liang
authors.initials: RL
authors.lastname: Chen
authors.affiliation: Mycological Research Center, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China. yinglong695489624@163.com.
authors.forename: Ying
authors.initials: Y
authors.lastname: Long
authors.affiliation: Mycological Research Center, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China. 18259088174@163.com.
authors.forename: Xiao
authors.initials: X
authors.lastname: Li
authors.affiliation: College of Food Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China. jyj1209@163.com.
authors.forename: Yu-Ji
authors.initials: YJ
authors.lastname: Jiang
authors.affiliation: Mycological Research Center, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China. mrcfafu@163.com.
authors.forename: Bao-Gui
authors.initials: BG
authors.lastname: Xie
mesh_terms: D055393:Flammulina / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism*; D048690:Fruiting Bodies, Fungal / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism*; D037102:Lectins / Q000737:chemistry / Q000378:metabolism*; D025282:Mycelium / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism*; D037121:Plant Lectins / Q000737:chemistry / Q000378:metabolism*
chemical_list: D037102:Lectins; D037121:Plant Lectins; C043696:jacalin
keywords: RNAi; mushroom; overexpression; transformation