title: High doses of ionizing radiation on bone repair: is there effect outside the irradiated site?
abstract: Local ionizing radiation causes damage to bone metabolism, it reduces blood supply and cellularity over time. Recent studies indicate that radiation promotes biological response outside the treatment field. The aim of this study was to investigate the effects of ionizing radiation on bone repair outside the irradiated field. Ten healthy male Wistar rats were used; and five animals were submitted to radiotherapy on the left femur. After 4 weeks, in all animals were created bone defects in the right and left femurs. Seven days after surgery, animals were euthanized. The femurs were removed and randomly divided into 3 groups (n=5): Control (C) (right femur of the non-irradiated animals); Local ionizing radiation (IR) (left femur of the irradiated animals); Contralateral ionizing radiation (CIR) (right femur of the irradiated animals). The femurs were processed and embedded in paraffin; and bone histologic sections were evaluated to quantify the bone neoformation. Histomorphometric analysis showed that there was no significant difference between groups C (24.6±7.04) and CIR (25.3±4.31); and IR group not showed bone neoformation. The results suggest that ionizing radiation affects bone repair, but does not interfere in bone repair distant from the primary irradiated site.
authors.affiliation: School of Dentistry, Oral & Maxillofacial Surgery and Implantology Department, University of Uberlândia, Departamento de Cirurgia e Traumatologia Buco-Maxilo-Facial, Avenida Pará s/n°, Campus Umuarama, Bloco 4T, Bairro Umuarama, Uberlândia, Minas Gerais, 38.400-902, Brazil.
authors.forename: Flaviana Soares
authors.initials: FS
authors.lastname: Rocha
authors.affiliation: Integrated Dental Clinic Program, School of Dentistry, University of Uberlândia, Departamento de Cirurgia e Traumatologia Buco-Maxilo-Facial, Avenida Pará s/n°, Campus Umuarama, Bloco 4T, Bairro Umuarama, Uberlândia, Minas Gerais, 38.400-902, Brazil.
authors.forename: Pâmella Coelho
authors.initials: PC
authors.lastname: Dias
authors.affiliation: Integrated Dental Clinic Program, School of Dentistry, University of Uberlândia, Departamento de Cirurgia e Traumatologia Buco-Maxilo-Facial, Avenida Pará s/n°, Campus Umuarama, Bloco 4T, Bairro Umuarama, Uberlândia, Minas Gerais, 38.400-902, Brazil.
authors.forename: Pedro Henrique Justino Oliveira
authors.initials: PH
authors.lastname: Limirio
authors.affiliation: School of Medicine, Radiotherapy Department, Federal University of Triângulo Mineiro, Avenida Getúlio Guarita, 130, Bairro Abadia, Uberaba, Minas Gerais, 38025-440, Brazil.
authors.forename: Vitor Carvalho
authors.initials: VC
authors.lastname: Lara
authors.affiliation: School of Dentistry, Oral & Maxillofacial Surgery and Implantology Department, University of Uberlândia, Departamento de Cirurgia e Traumatologia Buco-Maxilo-Facial, Avenida Pará s/n°, Campus Umuarama, Bloco 4T, Bairro Umuarama, Uberlândia, Minas Gerais, 38.400-902, Brazil.
authors.forename: Jonas Dantas
authors.initials: JD
authors.lastname: Batista
authors.affiliation: Biomedical Science Institute, University of Uberlândia, Departamento de Morfologia, Avenida Pará 1720, Campus Umuarama, Bloco 2B, Bairro Umuarama, Uberlândia, Minas Gerais, 38.400-902, Brazil. Electronic address: pauladechichi@ufu.br.
authors.forename: Paula
authors.initials: P
authors.lastname: Dechichi
mesh_terms: D000818:Animals; D001861:Bone Regeneration / Q000502:physiology / Q000528:radiation effects*; D004195:Disease Models, Animal; D005269:Femur / Q000378:metabolism / Q000528:radiation effects*; D008297:Male; D011839:Radiation, Ionizing; D051381:Rats; D017208:Rats, Wistar; D014945:Wound Healing / Q000502:physiology / Q000528:radiation effects*
keywords: Bone regeneration; Ionizing; Irradiated site; Radiation