title: The effects of SOST on implant osseointegration in ovariectomy osteoporotic mice.
abstract: OBJECTIVE
  Osteoporosis is a risk factor for implant fixation failure. The inhibition of sclerostin effectively improves bone formation and bone remodeling. Therefore, this study investigated whether SOST deficiency enhances the osseointegration of implants in a mouse model of osteoporosis induced by ovariectomy (OVX).
  
  
  METHODS
  Osteoporosis was induced in female C57BL/6 and SOST deficient mice by OVX. Titanium implants were placed in the bilateral distal aspects of the femurs. Implants underwent sandblasting and acid-etching after which the structure, surface roughness and chemical components were investigated using scanning electron microscopy (SEM) and energy spectrum analyses. Undecalcified slices, μ-CT, histology analyses and mechanical tests were used to evaluate the osseointegration of implants. The results were compared using one-way ANOVA between four groups.
  
  
  RESULTS
  Sandblasting and acid-etching increased the roughness of the implants. OVX surgery reduced bone formation around the implants in both WT and SOST-/-mice. However, implant osseointegration was significantly improved in the SOST-/-OVX mice compared to the WT OVX mice.
  
  
  CONCLUSIONS
  Inhibition of the SOST gene improved implant fixation in the OVX osteoporotic mice, which suggests a strategy for enhancing implant osseointegration in clinical patients with osteoporosis.
authors.affiliation: Department of Orthodontics and Pediatric Dentistry, State Key Laboratory of Oral Disease, West China School of Stomatology, Sichuan University, 14, 3rd Sec, Renminnan Rd, Chengdu, Sichuan 610041, China. Electronic address: shurui@scu.edu.cn.
authors.forename: Rui
authors.initials: R
authors.lastname: Shu
authors.affiliation: Department of Orthodontics, Chongqing Key Laboratory of Oral Diseases and Biomedical Sciences, Chongqing Municipal Key Laboratory of Oral Biomedical Engineering of Higher Education, Stomatological Hospital of Chongqing Medical University, Chongqing, China. Electronic address: 75974387@qq.com.
authors.forename: Dongqing
authors.initials: D
authors.lastname: Ai
authors.affiliation: Department of Orthodontics and Pediatric Dentistry, State Key Laboratory of Oral Disease, West China School of Stomatology, Sichuan University, 14, 3rd Sec, Renminnan Rd, Chengdu, Sichuan 610041, China. Electronic address: baiding@scu.edu.cn.
authors.forename: Ding
authors.initials: D
authors.lastname: Bai
authors.affiliation: Department of Orthodontics, Chongqing Key Laboratory of Oral Diseases and Biomedical Sciences, Chongqing Municipal Key Laboratory of Oral Biomedical Engineering of Higher Education, Stomatological Hospital of Chongqing Medical University, Chongqing, China. Electronic address: Soongjl@163.com.
authors.forename: Jinlin
authors.initials: J
authors.lastname: Song
authors.affiliation: Department of Orthodontics and Pediatric Dentistry, State Key Laboratory of Oral Disease, West China School of Stomatology, Sichuan University, 14, 3rd Sec, Renminnan Rd, Chengdu, Sichuan 610041, China. Electronic address: 195976600@qq.com.
authors.forename: Mengyuan
authors.initials: M
authors.lastname: Zhao
authors.affiliation: Department of Orthodontics and Pediatric Dentistry, State Key Laboratory of Oral Disease, West China School of Stomatology, Sichuan University, 14, 3rd Sec, Renminnan Rd, Chengdu, Sichuan 610041, China. Electronic address: xhan@scu.edu.cn.
authors.forename: Xianglong
authors.initials: X
authors.lastname: Han
mesh_terms: D048868:Adaptor Proteins, Signal Transducing; D000818:Animals; D016723:Bone Remodeling / Q000187:drug effects; D004195:Disease Models, Animal; D005260:Female; D005269:Femur / Q000187:drug effects / Q000293:injuries; D006023:Glycoproteins / Q000235:genetics / Q000494:pharmacology*; D036341:Intercellular Signaling Peptides and Proteins; D051379:Mice; D008810:Mice, Inbred C57BL; D016348:Osseointegration / Q000187:drug effects*; D010012:Osteogenesis / Q000187:drug effects; D010024:Osteoporosis / Q000473:pathology* / Q000601:surgery; D010052:Ovariectomy; D019736:Prostheses and Implants; D013499:Surface Properties; D014025:Titanium / Q000737:chemistry
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D006023:Glycoproteins; D036341:Intercellular Signaling Peptides and Proteins; C486030:Sost protein, mouse; D014025:Titanium
keywords: Implant; Osseointegration; Osteoporosis; Sclerostin