title: Enhanced Respiratory Chain Supercomplex Formation in Response to Exercise in Human Skeletal Muscle.
abstract: Mitochondrial dysfunction is a hallmark of multiple metabolic complications. Physical activity is known to increase mitochondrial content in skeletal muscle, counteracting age-related decline in muscle function and protecting against metabolic and cardiovascular complications. Here, we investigated the effect of 4 months of exercise training on skeletal muscle mitochondria electron transport chain complexes and supercomplexes in 26 healthy, sedentary older adults. Exercise differentially modulated respiratory complexes. Complex I was the most upregulated complex and not stoichiometrically associated to the other complexes. In contrast to the other complexes, complex I was almost exclusively found assembled in supercomplexes in muscle mitochondria. Overall, supercomplex content was increased after exercise. In particular, complexes I, III, and IV were redistributed to supercomplexes in the form of I+III2+IV. Taken together, our results provide the first evidence that exercise affects the stoichiometry of supercomplex formation in humans and thus reveal a novel adaptive mechanism for increased energy demand.
authors.affiliation: Aging and Muscle Metabolism Laboratory, Department of Physiology, University of Lausanne, Lausanne 1005, Switzerland.
authors.forename: Chiara
authors.initials: C
authors.lastname: Greggio
authors.affiliation: Laboratory of Integrative and Systems Physiology, École Polytechnique Fédérale de Lausanne, Lausanne 1015, Switzerland.
authors.forename: Pooja
authors.initials: P
authors.lastname: Jha
authors.affiliation: Nestlé Institute of Health Sciences, Lausanne 1015, Switzerland.
authors.forename: Sameer S
authors.initials: SS
authors.lastname: Kulkarni
authors.affiliation: Aging and Muscle Metabolism Laboratory, Department of Physiology, University of Lausanne, Lausanne 1005, Switzerland.
authors.forename: Sylviane
authors.initials: S
authors.lastname: Lagarrigue
authors.affiliation: Aging and Muscle Metabolism Laboratory, Department of Physiology, University of Lausanne, Lausanne 1005, Switzerland.
authors.forename: Nicholas T
authors.initials: NT
authors.lastname: Broskey
authors.affiliation: Nestlé Institute of Health Sciences, Lausanne 1015, Switzerland.
authors.forename: Marie
authors.initials: M
authors.lastname: Boutant
authors.affiliation: Laboratory of Integrative and Systems Physiology, École Polytechnique Fédérale de Lausanne, Lausanne 1015, Switzerland.
authors.forename: Xu
authors.initials: X
authors.lastname: Wang
authors.affiliation: Aging and Muscle Metabolism Laboratory, Department of Physiology, University of Lausanne, Lausanne 1005, Switzerland; Institute of Sports Sciences, University of Lausanne, Lausanne 1005, Switzerland.
authors.forename: Sonia
authors.initials: S
authors.lastname: Conde Alonso
authors.affiliation: Aging and Muscle Metabolism Laboratory, Department of Physiology, University of Lausanne, Lausanne 1005, Switzerland.
authors.forename: Emmanuel
authors.initials: E
authors.lastname: Ofori
authors.affiliation: Laboratory of Integrative and Systems Physiology, École Polytechnique Fédérale de Lausanne, Lausanne 1015, Switzerland. Electronic address: admin.auwerx@epfl.ch.
authors.forename: Johan
authors.initials: J
authors.lastname: Auwerx
authors.affiliation: Nestlé Institute of Health Sciences, Lausanne 1015, Switzerland. Electronic address: carlos.cantoalvarez@rd.nestle.com.
authors.forename: Carles
authors.initials: C
authors.lastname: Cantó
authors.affiliation: Aging and Muscle Metabolism Laboratory, Department of Physiology, University of Lausanne, Lausanne 1005, Switzerland; Institute of Sports Sciences, University of Lausanne, Lausanne 1005, Switzerland; Service of Endocrinology, Diabetology, and Metabolism, Department of Medicine, Lausanne University Hospital, Lausanne 1011, Switzerland. Electronic address: francesca.amati@unil.ch.
authors.forename: Francesca
authors.initials: F
authors.lastname: Amati
mesh_terms: D050154:Adiposity; D000368:Aged; D000375:Aging / Q000378:metabolism; D019069:Cell Respiration; D045222:Electron Transport Chain Complex Proteins / Q000378:metabolism*; D015444:Exercise / Q000502:physiology*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D018482:Muscle, Skeletal / Q000502:physiology*; D010100:Oxygen / Q000378:metabolism
chemical_list: D045222:Electron Transport Chain Complex Proteins; D010100:Oxygen
keywords: aging; electron transport chain; endurance training; exercise intervention; human; mitochondria; pre-/post-design; respiratory complex; skeletal muscle; supercomplex