title: Expression analysis and clinical significance of eIF4E, VEGF-C, E-cadherin and MMP-2 in colorectal adenocarcinoma.
abstract: The underlying mechanisms of colorectal carcinoma (CRC) metastasis remain to be elucidated. The aim of this study is to investigate clinical significance and the expression of eIF4E, VEGF-C, MMP-2, and E-cadherin in the CRC metastasis. We investigated their expressions in 108 patients, analyzed the relationships between their expressions in CRC and evaluated the relationships between their expressions and clinical pathogenic parameters. Furthermore, their roles in patient survival and in CRC metastasis were also investigated. We found that eIF4E, VEGF-C and MMP-2 were up-regulated in CRC, and their expression frequencies (EFs) were higher in cancerous tissues than in adjacent normal tissues. The EF of E-cadherin is lower in cancerous tissues than in adjacent normal tissues. Totally, their EFs were not associated with sex and age of patient, however, their EFs were associated with tumor differentiation, the depth of invasion, lymph node metastasis and tumor stages. Furthermore, eIF4E, VEGF-C, and MMP-2 shortened and E-cadherin prolonged survival in patient-derived CRC xenografts. Similarly, eIF4E, VEGF-C, and MMP-2 promoted and E-cadherin suppressed the lung metastasis of CRC cells. In addition, knockdown of eIF4E inhibited migration of CRC cells, downregulated VEGF-C, MMP-2 and upregulated E-cadherin. In conclusion, eIF4E promoted CRC metastasis via up-regulating the expression of VEGF-C, MMP-2 and suppressing E-cadherin.
authors.affiliation: Department of Pathology, Chongqing Medical University, Chongqing 400016, China.
authors.forename: Minna
authors.initials: M
authors.lastname: Gao
authors.affiliation: College of Basic Medicine, Chongqing Medical University, Chongqing 400016, China.
authors.forename: Xiong
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Department of Critical Care Medicine, Yongchuan Hospital, Chongqing Medical University, Chongqing 402160, China.
authors.forename: Dan
authors.initials: D
authors.lastname: Li
authors.affiliation: Department of Infectious diseases, Yongchuan Hospital, Chongqing Medical University, Chongqing 402160, China.
authors.forename: Ping
authors.initials: P
authors.lastname: He
authors.affiliation: Department of Infectious diseases, Yongchuan Hospital, Chongqing Medical University, Chongqing 402160, China.
authors.forename: Wenguang
authors.initials: W
authors.lastname: Tian
authors.affiliation: Department of Gastroenterology, Yongchuan Hospital, Chongqing Medical University, Chongqing 402160, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Zeng
mesh_terms: D000230:Adenocarcinoma / Q000201:enzymology* / Q000235:genetics / Q000556:secondary; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000818:Animals; D015703:Antigens, CD; D014408:Biomarkers, Tumor / Q000235:genetics / Q000378:metabolism*; D015820:Cadherins / Q000235:genetics / Q000378:metabolism*; D002454:Cell Differentiation; D045744:Cell Line, Tumor; D002465:Cell Movement; D015179:Colorectal Neoplasms / Q000201:enzymology* / Q000235:genetics / Q000473:pathology; D039561:Eukaryotic Initiation Factor-4E / Q000235:genetics / Q000378:metabolism*; D005260:Female; D006801:Humans; D008175:Lung Neoplasms / Q000201:enzymology* / Q000235:genetics / Q000556:secondary; D008207:Lymphatic Metastasis; D008297:Male; D020778:Matrix Metalloproteinase 2 / Q000235:genetics / Q000378:metabolism*; D016688:Mice, Inbred NOD; D016513:Mice, SCID; D008875:Middle Aged; D009361:Neoplasm Invasiveness; D009367:Neoplasm Staging; D009368:Neoplasm Transplantation; D034622:RNA Interference; D013997:Time Factors; D014162:Transfection; D015854:Up-Regulation; D042582:Vascular Endothelial Growth Factor C / Q000235:genetics / Q000378:metabolism*; D055815:Young Adult
chemical_list: D015703:Antigens, CD; D014408:Biomarkers, Tumor; C513384:CDH1 protein, human; D015820:Cadherins; D039561:Eukaryotic Initiation Factor-4E; C467486:VEGFC protein, human; D042582:Vascular Endothelial Growth Factor C; C522361:MMP2 protein, human; D020778:Matrix Metalloproteinase 2
keywords: E-cadherin; MMP-2; VEGF-C; colorectal cancer; eIF4E