title: Regulation of turkey myogenic satellite cell migration by MicroRNAs miR-128 and miR-24.
abstract: Myogenic satellite cells are an adult stem cell responsible for all post-hatch muscle growth in poultry. As a stem cell population, satellite cells are highly heterogeneous, but the origin of this heterogeneity remains unclear. Heterogeneity is, in part, regulated by gene expression. One method of endogenous gene regulation that may contribute to heterogeneity is microRNAs (miRNAs). Two miRNAs previously shown to regulate poultry myogenic satellite cell proliferation and differentiation, miR-128 and miR-24, were studied to determine if they also affected satellite cell migration. Satellite cell migration is an essential step for both proliferation and differentiation. During proliferation, satellite cells will migrate and align to form new myofibers or donate their nuclei to existing myofibers leading to muscle fiber hypertrophy or regeneration. Transient transfection of miRNA specific mimics to each miRNA reduced migration of satellite cells following a cell culture scratch at 72 h of proliferation when the cultures were 90 to 100% confluent. However, only the migration in cells transfected with miR-24 mimics at 24 and 30 h following the scratch was significantly reduced (P ≤ 0.05) to around 70% of the distance migrated by controls. Alternately, transfection with inhibitors specific to miR-128 or miR-24 significantly (P ≤ 0.05) increased migration between 147 and 252% compared to their controls between 24 and 48 h following the scratch. These data demonstrate that miR-128 and miR-24 play a role in myogenic satellite cell migration, which will impact muscle development and growth.
authors.forename: S G
authors.initials: SG
authors.lastname: Velleman
authors.forename: R L
authors.initials: RL
authors.lastname: Harding
mesh_terms: D000818:Animals; D002465:Cell Movement / Q000187:drug effects / Q000235:genetics; D049109:Cell Proliferation; D002478:Cells, Cultured; D035683:MicroRNAs / Q000037:antagonists & inhibitors / Q000235:genetics*; D024510:Muscle Development / Q000187:drug effects / Q000235:genetics; D012038:Regeneration / Q000187:drug effects / Q000235:genetics; D032496:Satellite Cells, Skeletal Muscle / Q000166:cytology* / Q000187:drug effects; D014422:Turkeys
chemical_list: D035683:MicroRNAs
keywords: Hypertrophy; micro RNA; migration; satellite cell; skeletal muscle