title: Gsy, a novel glucansucrase from Leuconostoc mesenteroides, mediates the formation of cell aggregates in response to oxidative stress.
abstract: Leuconostoc mesenteroides is a member of lactic acid bacteria (LAB) with wide applications in the food and medical industries. Species in the genus Leuconostoc are catalase-negative and generally regarded as facultative anaerobic or aerotolerant organisms. Despite their extensive use in industry, certain issues concerning the aerobic life of L. mesenteroides, e.g., the mechanism involved in the tolerance to oxygen, remain to be addressed. In this manuscript, a survival strategy employed by L. mesenteroides BD3749 in response to oxidative stress was elucidated. BD3749 cells cultivated in medium with sucrose available synthesized large amounts of exopolysaccharides, mostly consisting of insoluble EPS. When BD3749 cells were challenged with oxidative stress, the amount of insoluble EPS was greatly enhanced. The synthesized EPSs reduced the accumulation of reactive oxygen species (ROS) in bacterial cells and improved their survival during chronic oxidative stress. Another study showed that Gsy, a novel glucansucrase in the GH70 family that is induced by sucrose and up-regulated following exposure to oxygen, was responsible for the synthesis of insoluble EPS. Gsy was subsequently demonstrated to play pivotal roles in the formation of aggregates to alleviate the detrimental effects on BD3749 cells exerted by oxygen.
authors.affiliation: State Key Laboratory of Dairy Biotechnology, Institute of Bright Dairy &Food Co., Ltd., 1518 West Jiangchang Road, Shanghai 200436, PR China.|Department of Food Science and Technology, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, PR China.
authors.forename: Minghui
authors.initials: M
authors.lastname: Yan
authors.affiliation: State Key Laboratory of Dairy Biotechnology, Institute of Bright Dairy &Food Co., Ltd., 1518 West Jiangchang Road, Shanghai 200436, PR China.
authors.forename: Jin
authors.initials: J
authors.lastname: Han
authors.affiliation: State Key Laboratory of Dairy Biotechnology, Institute of Bright Dairy &Food Co., Ltd., 1518 West Jiangchang Road, Shanghai 200436, PR China.
authors.forename: Xiaofen
authors.initials: X
authors.lastname: Xu
authors.affiliation: School of Marine Sciences, Key Laboratory of Applied Marine Biotechnology (Ministry of Education), Ningbo University, Ningbo, 315211, PR China.
authors.forename: Lianliang
authors.initials: L
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Dairy Biotechnology, Institute of Bright Dairy &Food Co., Ltd., 1518 West Jiangchang Road, Shanghai 200436, PR China.
authors.forename: Caixia
authors.initials: C
authors.lastname: Gao
authors.affiliation: Shanghai-MOST Key Laboratory of Health and Disease Genomics, Chinese National Human Genome Center at Shanghai, Shanghai Zhang Jiang Hi-TechPark, 250 Bi-Bo Road, Shanghai 201203PR China.
authors.forename: Huajun
authors.initials: H
authors.lastname: Zheng
authors.affiliation: School of Food Science and Technology, Jiangnan University, 1800 Lihu Rd,Wuxi,214122, PR China.
authors.forename: Yunxia
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Department of Analytical Chemistry, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai, 201203, PR China.
authors.forename: Yimin
authors.initials: Y
authors.lastname: Tao
authors.affiliation: Department of Analytical Chemistry, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai, 201203, PR China.
authors.forename: Hu
authors.initials: H
authors.lastname: Zhou
authors.affiliation: Department of Food Science and Technology, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, PR China.
authors.forename: Yunfei
authors.initials: Y
authors.lastname: Li
authors.affiliation: State Key Laboratory of Dairy Biotechnology, Institute of Bright Dairy &Food Co., Ltd., 1518 West Jiangchang Road, Shanghai 200436, PR China.
authors.forename: Zhengjun
authors.initials: Z
authors.lastname: Wu
mesh_terms: D016695:Glycosyltransferases / Q000378:metabolism*; D000070007:Leuconostoc mesenteroides / Q000378:metabolism*; D018384:Oxidative Stress / Q000502:physiology*; D010100:Oxygen / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism; D013395:Sucrose / Q000378:metabolism; D015854:Up-Regulation / Q000502:physiology
chemical_list: D017382:Reactive Oxygen Species; D013395:Sucrose; D016695:Glycosyltransferases; C033837:alternansucrase; D010100:Oxygen