title: Intracellular distribution and stability of a luminescent rhenium(i) tricarbonyl tetrazolato complex using epifluorescence microscopy in conjunction with X-ray fluorescence imaging.
abstract: Optical epifluorescence microscopy was used in conjunction with X-ray fluorescence imaging to monitor the stability and intracellular distribution of the luminescent rhenium(i) complex fac-[Re(CO)3(phen)L], where phen = 1,10-phenathroline and L = 5-(4-iodophenyl)tetrazolato, in 22Rv1 cells. The rhenium complex showed no signs of ancillary ligand dissociation, a conclusion based on data obtained via X-ray fluorescence imaging aligning iodine and rhenium distributions. A diffuse reticular localisation was detected for the complex in the nuclear/perinuclear region of cells, by either optical or X-ray fluorescence imaging techniques. X-ray fluorescence also showed that the rhenium complex disrupted the homeostasis of some biologically relevant elements, such as chlorine, potassium and zinc.
authors.affiliation: Department of Chemistry, The University of Adelaide, SA 5005, Australia. hugh.harris@adelaide.edu.au.
authors.forename: J L
authors.initials: JL
authors.lastname: Wedding
authors.affiliation: Department of Chemistry, The University of Adelaide, SA 5005, Australia. hugh.harris@adelaide.edu.au.
authors.forename: H H
authors.identifier: 0000-0002-3472-8628
authors.initials: HH
authors.lastname: Harris
authors.affiliation: School of Pharmacy and Medical Sciences, University of South Australia, Adelaide, South Australia 5005, Australia.
authors.forename: C A
authors.initials: CA
authors.lastname: Bader
authors.affiliation: School of Pharmacy and Medical Sciences, University of South Australia, Adelaide, South Australia 5005, Australia.
authors.forename: S E
authors.initials: SE
authors.lastname: Plush
authors.affiliation: School of Chemistry, The University of Sydney, NSW 2006, Australia.
authors.forename: R
authors.initials: R
authors.lastname: Mak
authors.affiliation: Department of Chemistry, Perth, Western Australia 6102, Australia. M.Massi@curtin.edu.au.
authors.forename: M
authors.initials: M
authors.lastname: Massi
authors.affiliation: School of Pharmacy and Medical Sciences, University of South Australia, Adelaide, South Australia 5005, Australia.
authors.forename: D A
authors.initials: DA
authors.lastname: Brooks
authors.affiliation: Advanced Photon Source, X-ray Science Division, Argonne National Laboratory, Argonne, IL 60439, USA.
authors.forename: B
authors.initials: B
authors.lastname: Lai
authors.affiliation: Advanced Photon Source, X-ray Science Division, Argonne National Laboratory, Argonne, IL 60439, USA.
authors.forename: S
authors.initials: S
authors.lastname: Vogt
authors.affiliation: Department of Chemistry, Perth, Western Australia 6102, Australia. M.Massi@curtin.edu.au.
authors.forename: M V
authors.initials: MV
authors.lastname: Werrett
authors.affiliation: Department of Chemistry, Perth, Western Australia 6102, Australia. M.Massi@curtin.edu.au.
authors.forename: P V
authors.initials: PV
authors.lastname: Simpson
authors.affiliation: Centre for Microscopy, Characterisation and Analysis, University of Western Australia, Crawley 6009 WA, Australia.
authors.forename: B W
authors.initials: BW
authors.lastname: Skelton
authors.affiliation: Department of Industrial Chemistry "Toso Montanari" - University of Bologna, viale del Risorgimento 4, Bologna 40136, Italy.
authors.forename: S
authors.initials: S
authors.lastname: Stagni
mesh_terms: D045744:Cell Line, Tumor; D056831:Coordination Complexes / Q000032:analysis*; D006801:Humans; D049408:Luminescent Agents / Q000032:analysis*; D008856:Microscopy, Fluorescence / Q000379:methods*; D061848:Optical Imaging / Q000379:methods*; D010618:Phenanthrolines / Q000032:analysis; D012211:Rhenium / Q000032:analysis*; D013777:Tetrazoles / Q000032:analysis*; D014965:X-Rays
chemical_list: D056831:Coordination Complexes; D049408:Luminescent Agents; D010618:Phenanthrolines; D013777:Tetrazoles; D012211:Rhenium