title: [Protective effect of interleukin 22 against hyperoxia-induced lung injury in neonatal rats].
abstract: Objective To study the protective effect of interleukin-22 (IL-22) against the hyperoxia-induced lung injury in neonatal SD rats. Methods The neonatal SD rats were randomized into control group, hyperoxia group and IL-22 (10 ng/g) treatment group. Each group was randomly divided into three subgroups of 1, 3, 7 days (n=9). Body mass in every group was detected; lung pathological changes were observed by HE staining; tumor necrosis factor α (TNF-α) in lung tissues was tested by quantitative real-time PCR; and IL-1β in plasma was measured by ELISA. Results After exposure to hyperoxia for 1 day, the body mass in the three groups showed no significant difference, and the structure of lung tissues were normal. After exposure to hyperoxia for 3 or 7 days, the body mass in the hyperoxia group was significantly lower, IL-1β in plasma was significantly enhanced, and the structure of lung tissues were destroyed, which aggravated with the time of hyperoxia exposure. TNF-α mRNA level increased obviously in the hyperoxia group. However, when treated with IL-22, TNF-α mRNA was significantly down-regulated in the treatment group. Conclusion IL-22 may play an protective role in hyperoxia-induced lung injury.
authors.affiliation: Department of Neonatology, Affiliated Hospital of Binzhou Medical University, Binzhou 256600, China.
authors.forename: Li
authors.initials: L
authors.lastname: Yuan
authors.affiliation: Department of Neonatology, Affiliated Hospital of Binzhou Medical University, Binzhou 256600, China. *Corresponding author, E-mail: liuxiuxiang99@163.com.
authors.forename: Xiuxiang
authors.initials: X
authors.lastname: Liu
authors.affiliation: Department of Neonatology, Affiliated Hospital of Binzhou Medical University, Binzhou 256600, China.
authors.forename: Ruiwei
authors.initials: R
authors.lastname: Gao
authors.affiliation: Department of Neonatology, Affiliated Hospital of Binzhou Medical University, Binzhou 256600, China.
authors.forename: Jinshuai
authors.initials: J
authors.lastname: Ma
authors.affiliation: Department of Neonatology, Affiliated Hospital of Binzhou Medical University, Binzhou 256600, China.
authors.forename: Suni
authors.initials: S
authors.lastname: Lu
authors.affiliation: Department of Neonatology, Affiliated Hospital of Binzhou Medical University, Binzhou 256600, China.
authors.forename: Haihong
authors.initials: H
authors.lastname: Zhang
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D004195:Disease Models, Animal; D018496:Hyperoxia / Q000150:complications* / Q000378:metabolism; D053583:Interleukin-1beta / Q000097:blood; D007378:Interleukins / Q000494:pharmacology* / Q000627:therapeutic use*; D055370:Lung Injury / Q000188:drug therapy* / Q000209:etiology*; D012333:RNA, Messenger; D051381:Rats; D017207:Rats, Sprague-Dawley; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics / Q000378:metabolism; D000097043:Interleukin-22
chemical_list: D053583:Interleukin-1beta; D007378:Interleukins; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha; D000097043:Interleukin-22