title: A Systematic Evaluation of Blood Serum and Plasma Pre-Analytics for Metabolomics Cohort Studies.
abstract: The recent thriving development of biobanks and associated high-throughput phenotyping studies requires the elaboration of large-scale approaches for monitoring biological sample quality and compliance with standard protocols. We present a metabolomic investigation of human blood samples that delineates pitfalls and guidelines for the collection, storage and handling procedures for serum and plasma. A series of eight pre-processing technical parameters is systematically investigated along variable ranges commonly encountered across clinical studies. While metabolic fingerprints, as assessed by nuclear magnetic resonance, are not significantly affected by altered centrifugation parameters or delays between sample pre-processing (blood centrifugation) and storage, our metabolomic investigation highlights that both the delay and storage temperature between blood draw and centrifugation are the primary parameters impacting serum and plasma metabolic profiles. Storing the blood drawn at 4 °C is shown to be a reliable routine to confine variability associated with idle time prior to sample pre-processing. Based on their fine sensitivity to pre-analytical parameters and protocol variations, metabolic fingerprints could be exploited as valuable ways to determine compliance with standard procedures and quality assessment of blood samples within large multi-omic clinical and translational cohort studies.
authors.affiliation: Univ Lyon, CNRS, Université Claude Bernard Lyon 1, ENS de Lyon, Institut des Sciences Analytiques UMR 5280, 5 rue de la Doua, F-69100 Villeurbanne, France. elodie.jobard@ens-lyon.fr.|Centre Léon Bérard, Département de Recherche Translationnelle et de l'Innovation, 28 rue Laënnec, 69373 Lyon, CEDEX 08, France. elodie.jobard@ens-lyon.fr.
authors.forename: Elodie
authors.initials: E
authors.lastname: Jobard
authors.affiliation: Centre Léon Bérard, Département d'oncologie Médicale, 28 rue Laënnec, 69373 Lyon, CEDEX 08, France. olivier.tredan@lyon.unicancer.fr.
authors.forename: Olivier
authors.initials: O
authors.lastname: Trédan
authors.affiliation: Centre Léon Bérard, Département de Recherche Translationnelle et de l'Innovation, Génomique des Cancers, 28 rue Laënnec, 69373 Lyon, CEDEX 08, France. deborah.postoly@lyon.unicancer.fr.
authors.forename: Déborah
authors.initials: D
authors.lastname: Postoly
authors.affiliation: Department of Medical Oncology, Gustave Roussy, Université Paris-Saclay, 94805 Villejuif, France. fabrice.andre@gustaveroussy.fr.
authors.forename: Fabrice
authors.initials: F
authors.lastname: André
authors.affiliation: R&D UNICANCER, 101 rue de Tolbiac, 75654 Paris, CEDEX 13, France. al-martin@unicancer.fr.
authors.forename: Anne-Laure
authors.initials: AL
authors.lastname: Martin
authors.affiliation: Univ Lyon, CNRS, Université Claude Bernard Lyon 1, ENS de Lyon, Institut des Sciences Analytiques UMR 5280, 5 rue de la Doua, F-69100 Villeurbanne, France. benedicte.elena@ens-lyon.fr.
authors.forename: Bénédicte
authors.initials: B
authors.lastname: Elena-Herrmann
authors.affiliation: Centre Léon Bérard, Département de Recherche Translationnelle et de l'Innovation, Génomique des Cancers, 28 rue Laënnec, 69373 Lyon, CEDEX 08, France. sandrine.boyault@lyon.unicancer.fr.
authors.forename: Sandrine
authors.initials: S
authors.lastname: Boyault
mesh_terms: D001800:Blood Specimen Collection / Q000379:methods / Q000592:standards; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D055432:Metabolomics / Q000379:methods* / Q000592:standards; D010949:Plasma / Q000737:chemistry*; D044967:Serum / Q000737:chemistry*
keywords: metabolomics; nuclear magnetic resonance; plasma; pre-analytics; quality control; serum