title: Serum miR-95-3p is a diagnostic and prognostic marker for osteosarcoma.
abstract: It has been demonstrated that microRNAs (miRNAs or miRs) can act as prognostic and diagnostic markers, and potential therapeutic targets. miR-95-3p has been reported to be downregulated in osteosarcoma tissues, but its potential as a serum biomarker has not been assessed in human osteosarcoma. The purpose of the present study was to examine the expression levels of miR-95-3p in serum of patients with osteosarcoma and to investigate the diagnostic and prognostic value of miR-95-3p. The serum levels of miR-95-3p in osteosarcoma patients were detected by a real-time quantitative reverse transcription-polymerase chain reaction assay. Associations between miR-95-3p expression and various clinicopathological characteristics were analyzed using Chi square test. Differences in patient survival were determined using the Kaplan-Meier method and a log-rank test. A Cox proportional hazards regression analysis was used for multivariate analyses of prognostic values. Compared to healthy controls, the expression levels of miR-95-3p in serum of osteosarcoma patients were significantly decreased (P< 0.0001). Low miR-95-3p expression had significant association with clinical stage (P< 0.001) and metastasis (P< 0.001). The Kaplan-Meier curve showed that patients with high miR-95-3p expression survived significantly longer than patients with low miR-95-3p expression (P= 0.017). Multivariate analysis demonstrated that miR-95-3p expression level (P= 0.014) was an independent prognostic biomarker for overall survival. Our findings suggested that down-expression of serum miR-95-3p might be associated with poor prognosis of osteosarcoma patients, suggesting that decreased expression of serum miR-95-3p may serve as a valuable diagnostic/prognostic marker for osteosarcoma patients.
authors.affiliation: Department of Radiology, Zhengzhou Orthopedics Hospital, Zhengzhou, China.
authors.forename: Junjie
authors.initials: J
authors.lastname: Niu
authors.affiliation: Department of Minimally Invasive Spine Surgery, Zhengzhou Orthopedics Hospital, No. 58 Longhai Road, Zhengzhou, 450000 Henan People's Republic of China.
authors.forename: Yibao
authors.initials: Y
authors.lastname: Sun
authors.affiliation: Zhengzhou Key Laboratory of Bone and Joint Imaging, Zhengzhou, China.
authors.forename: Qiaoge
authors.initials: Q
authors.lastname: Guo
authors.affiliation: Department of Radiology, Zhengzhou Second Hospital, Zhengzhou, China.
authors.forename: Dongju
authors.initials: D
authors.lastname: Niu
authors.affiliation: Department of Function, Zhengzhou Orthopedics Hospital, Zhengzhou, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Liu
keywords: Diagnosis; Osteosarcoma; Prognosis; Serum; miR-95-3p