title: Development of a Novel and Rapid Fully Automated Genetic Testing System.
abstract: We have developed a rapid genetic testing system integrating nucleic acid extraction, purification, amplification, and detection in a single cartridge. The system performs real-time polymerase chain reaction (PCR) after nucleic acid purification in a fully automated manner. RNase P, a housekeeping gene, was purified from human nasal epithelial cells using silica-coated magnetic beads and subjected to real-time PCR using a novel droplet-real-time-PCR machine. The process was completed within 13 min. This system will be widely applicable for research and diagnostic uses.
authors.affiliation: Core Technology Development Center, Corporate Research and Development Div., Seiko Epson Corporation.
authors.forename: Masayuki
authors.initials: M
authors.lastname: Uehara
mesh_terms: D001331:Automation; D005820:Genetic Testing / Q000295:instrumentation* / Q000379:methods* / Q000639:trends; D006801:Humans; D009297:Nasal Mucosa / Q000166:cytology; D060888:Real-Time Polymerase Chain Reaction; D043262:Ribonuclease P / Q000235:genetics; D013997:Time Factors
chemical_list: D043262:Ribonuclease P