title: Electron spin resonance and fluorescence imaging assisted electrochemical approach for accurate and comprehensive monitoring of cellular hydrogen peroxide dynamics.
abstract: Dynamic alteration in the levels of cellular hydrogen peroxide (H2O2) is closely related to a variety of human diseases, as well as signal transduction pathways that regulate cell survival and death. Although qualitative or quantitative methods are available for measuring either intra- or extra-cellular H2O2levels, accurate and comprehensive in situ detection of the real-time H2O2dynamics of living cells remains a significant challenge. To solve this problem, a novel multi-dimensional in situ cell assay platform combining electrochemistry, electron spin resonance (ESR) and optical imaging is designed. In this platform, the real-time concentration of extracellular H2O2released from stimulated cells can be accurately detected by ESR assisted chronoamperometry, while the level of intracellular H2O2is simultaneously monitored via the incorporated fluorescence imaging. Accurately and simultaneously analyzing the level variations of extra- and intra-cellular reactive oxygen species based on our assay platform can complement each other for further precise and in-depth investigation of their membrane transport and related cellular signaling, which will benefit disease diagnosis and treatment.
authors.affiliation: CAS Center for Excellence in Nanoscience, CAS Key Laboratory for Nanosystem and Hierarchical Fabrication, National Center for Nanoscience and Technology, Beijing 100190, P. R. China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Xin
authors.affiliation: CAS Center for Excellence in Nanoscience, CAS Key Laboratory for Nanosystem and Hierarchical Fabrication, National Center for Nanoscience and Technology, Beijing 100190, P. R. China and University of Chinese Academy of Science, Beijing 100049, P. R. China. gongjr@nanoctr.cn.
authors.forename: Qian
authors.initials: Q
authors.lastname: Liu
authors.affiliation: CAS Center for Excellence in Nanoscience, CAS Key Laboratory for Nanosystem and Hierarchical Fabrication, National Center for Nanoscience and Technology, Beijing 100190, P. R. China and University of Chinese Academy of Science, Beijing 100049, P. R. China. gongjr@nanoctr.cn.
authors.forename: Hameed
authors.initials: H
authors.lastname: Shah
authors.affiliation: CAS Center for Excellence in Nanoscience, CAS Key Laboratory for Nanosystem and Hierarchical Fabrication, National Center for Nanoscience and Technology, Beijing 100190, P. R. China.
authors.forename: Jian Ru
authors.initials: JR
authors.lastname: Gong
mesh_terms: D055664:Electrochemical Techniques / Q000379:methods; D004566:Electrodes; D004578:Electron Spin Resonance Spectroscopy / Q000379:methods; D006367:HeLa Cells; D006801:Humans; D006861:Hydrogen Peroxide / Q000032:analysis* / Q000378:metabolism*; D061848:Optical Imaging / Q000379:methods
chemical_list: D006861:Hydrogen Peroxide