title: Improved detection ofShigellausingEscherichia colimedium enrichment: Polymerase chain reaction from stool samples.
abstract: Laboratory diagnosis of shigellosis using conventional culture technique is limited by lower sensitivity and higher turnaround time. Here, we have evaluated the role of polymerase chain reaction from stool samples after enrichment in Escherichia coli medium for detection of Shigellae. The technique not only increased the sensitivity but also decreased the turnaround time.
authors.affiliation: Department of Microbiology, Dr. RPGMC, Kangra, Himachal Pradesh, India.
authors.forename: P K
authors.initials: PK
authors.lastname: Gupta
authors.affiliation: Department of Microbiology, SDM Medical College, Dharwad, Karnataka, India.
authors.forename: S B
authors.initials: SB
authors.lastname: Appannanavar
authors.affiliation: Department of Medical Microbiology, Post Graduate Institute of Medical Education and Research, Chandigarh, India.
authors.forename: B
authors.initials: B
authors.lastname: Mohan
authors.affiliation: Department of Medical Microbiology, Post Graduate Institute of Medical Education and Research, Chandigarh, India.
authors.forename: N
authors.initials: N
authors.lastname: Taneja
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001431:Bacteriological Techniques / Q000379:methods*; D002648:Child; D002675:Child, Preschool; D004405:Dysentery, Bacillary / Q000175:diagnosis*; D005243:Feces / Q000382:microbiology*; D005260:Female; D006801:Humans; D007223:Infant; D008297:Male; D008875:Middle Aged; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D012760:Shigella / Q000302:isolation & purification*; D013048:Specimen Handling / Q000379:methods*; D013997:Time Factors; D055815:Young Adult