title: Subtilosin Prevents Biofilm Formation by Inhibiting Bacterial Quorum Sensing.
abstract: Subtilosin, the cyclic lantibiotic protein produced by Bacillus subtilis KATMIRA1933, targets the surface receptor and electrostatically binds to the bacterial cell membrane. In this study, subtilosin was purified using ammonium sulfate ((NH4)2SO4) precipitation and purified via column chromatography. Subtilosin's antibacterial minimum and sub-minimum inhibitory concentrations (MIC and sub-MIC) and anti-biofilm activity (biofilm prevention) were established. Subtilosin was evaluated as a quorum sensing (QS) inhibitor in Gram-positive bacteria using Fe(III) reduction assay. In Gram-negative bacteria, subtilosin was evaluated as a QS inhibitor utilizing Chromobacterium voilaceum as a microbial reporter. The results showed that Gardnerella vaginalis was more sensitive to subtilosin with MIC of 6.25 μg/mL when compared to Listeria monocytogenes (125 μg/mL). The lowest concentration of subtilosin, at which more than 90% of G. vaginalis biofilm was inhibited without effecting the growth of planktonic cells, was 0.78 μg/mL. About 80% of L. monocytogenes and more than 60% of Escherichia coli biofilm was inhibited when 15.1 μg/mL of subtilosin was applied. Subtilosin with 7.8-125 μg/mL showed a significant reduction in violacein production without any inhibitory effect on the growth of C. violaceum. Subtilosin at 3 and 4 μg/mL reduced the level of Autoinducer-2 (AI-2) production in G. vaginalis. However, subtilosin did not influence AI-2 production by L. monocytogenes at sub-MICs of 0.95-15.1 μg/mL. To our knowledge, this is the first report exploring the relationship between biofilm prevention and quorum sensing inhibition in G. vaginalis using subtilosin as a quorum sensing inhibitor.
authors.affiliation: Department of Biochemistry and Microbiology, Rutgers State University, New Brunswick, NJ, 08901, USA.|Department of Microbiology, Veterinary College, Diyala University, Baqubah, Iraq.
authors.forename: Ammar
authors.initials: A
authors.lastname: Algburi
authors.affiliation: Department of Life Sciences and Technology, Beuth University of Applied Sciences, Berlin, Germany.
authors.forename: Saskia
authors.initials: S
authors.lastname: Zehm
authors.affiliation: Health Promoting Naturals Laboratory, School of Environmental and Biological Sciences, Rutgers State University, New Brunswick, NJ, USA.
authors.forename: Victoria
authors.initials: V
authors.lastname: Netrebov
authors.affiliation: D. I. Ivanovsky Academy of Biology and Biotechnology, Southern Federal University, Rostov-on-Don, Russia.
authors.forename: Anzhelica B
authors.initials: AB
authors.lastname: Bren
authors.affiliation: D. I. Ivanovsky Academy of Biology and Biotechnology, Southern Federal University, Rostov-on-Don, Russia.
authors.forename: Vladimir
authors.initials: V
authors.lastname: Chistyakov
authors.affiliation: School of Environmental and Biological Sciences, Rutgers State University, New Brunswick, NJ, USA. tchikindas@aesop.rutgers.edu.|Center for Digestive Health, New Jersey Institute for Food, Nutrition and Health, New Brunswick, NJ, USA. tchikindas@aesop.rutgers.edu.|Rutgers State University, 65 Dudley Road, New Brunswick, NJ, 08901, USA. tchikindas@aesop.rutgers.edu.
authors.forename: Michael L
authors.initials: ML
authors.lastname: Chikindas
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001412:Bacillus subtilis / Q000737:chemistry; D001430:Bacteriocins / Q000302:isolation & purification / Q000494:pharmacology*; D018441:Biofilms / Q000187:drug effects*; D006090:Gram-Negative Bacteria / Q000187:drug effects* / Q000502:physiology; D006094:Gram-Positive Bacteria / Q000187:drug effects* / Q000502:physiology; D010456:Peptides, Cyclic / Q000302:isolation & purification / Q000494:pharmacology*; D053038:Quorum Sensing / Q000187:drug effects*
chemical_list: D000900:Anti-Bacterial Agents; D001430:Bacteriocins; D010456:Peptides, Cyclic; C047828:sboA protein, Bacillus subtilis
keywords: Biofilm inhibition; Gardnerella vaginalis; Quorum sensing; Subtilosin