title: Accelerated craniofacial bone regeneration through dense collagen gel scaffolds seeded with dental pulp stem cells.
abstract: Therapies using mesenchymal stem cell (MSC) seeded scaffolds may be applicable to various fields of regenerative medicine, including craniomaxillofacial surgery. Plastic compression of collagen scaffolds seeded with MSC has been shown to enhance the osteogenic differentiation of MSC as it increases the collagen fibrillary density. The aim of the present study was to evaluate the osteogenic effects of dense collagen gel scaffolds seeded with mesenchymal dental pulp stem cells (DPSC) on bone regeneration in a rat critical-size calvarial defect model. Two symmetrical full-thickness defects were created (5 mm diameter) and filled with either a rat DPSC-containing dense collagen gel scaffold (n = 15), or an acellular scaffold (n = 15). Animals were imaged in vivo by microcomputer tomography (Micro-CT) once a week during 5 weeks, whereas some animals were sacrificed each week for histology and histomorphometry analysis. Bone mineral density and bone micro-architectural parameters were significantly increased when DPSC-seeded scaffolds were used. Histological and histomorphometrical data also revealed significant increases in fibrous connective and mineralized tissue volume when DPSC-seeded scaffolds were used, associated with expression of type I collagen, osteoblast-associated alkaline phosphatase and osteoclastic-related tartrate-resistant acid phosphatase. Results demonstrate the potential of DPSC-loaded-dense collagen gel scaffolds to benefit of bone healing process.
authors.affiliation: EA 2496 Orofacial pathologies, imaging and biotherapies, Dental School Faculty, University Paris Descartes, and Life imaging Platform (PIV), Montrouge, France.|Department of Periodontology, Service of Odontology, Rothschild Hospital, AP-HP, University Denis Diderot, U.F.R. of Odontology, Paris, France.
authors.forename: Frédéric
authors.initials: F
authors.lastname: Chamieh
authors.affiliation: EA 2496 Orofacial pathologies, imaging and biotherapies, Dental School Faculty, University Paris Descartes, and Life imaging Platform (PIV), Montrouge, France.|Department of Odontology, University Hospitals, AP-HP, Paris, France.
authors.forename: Anne-Margaux
authors.initials: AM
authors.lastname: Collignon
authors.affiliation: EA 2496 Orofacial pathologies, imaging and biotherapies, Dental School Faculty, University Paris Descartes, and Life imaging Platform (PIV), Montrouge, France.|Department of Periodontology, Service of Odontology, Rothschild Hospital, AP-HP, University Denis Diderot, U.F.R. of Odontology, Paris, France.
authors.forename: Benjamin R
authors.initials: BR
authors.lastname: Coyac
authors.affiliation: EA 2496 Orofacial pathologies, imaging and biotherapies, Dental School Faculty, University Paris Descartes, and Life imaging Platform (PIV), Montrouge, France.
authors.forename: Julie
authors.initials: J
authors.lastname: Lesieur
authors.affiliation: EA 2496 Orofacial pathologies, imaging and biotherapies, Dental School Faculty, University Paris Descartes, and Life imaging Platform (PIV), Montrouge, France.
authors.forename: Sandy
authors.initials: S
authors.lastname: Ribes
authors.affiliation: EA 2496 Orofacial pathologies, imaging and biotherapies, Dental School Faculty, University Paris Descartes, and Life imaging Platform (PIV), Montrouge, France.
authors.forename: Jérémy
authors.initials: J
authors.lastname: Sadoine
authors.affiliation: EA 2496 Orofacial pathologies, imaging and biotherapies, Dental School Faculty, University Paris Descartes, and Life imaging Platform (PIV), Montrouge, France.
authors.forename: Annie
authors.initials: A
authors.lastname: Llorens
authors.affiliation: INSERM U1148, Laboratory of Vascular Translational Science, University Paris Diderot, University Paris 13, Sorbonne Paris Cité, X Bichat Hospital, and Département Hospitalo-Universitaire (DHU) FIRE, F-75018 Paris, France.
authors.forename: Antonino
authors.initials: A
authors.lastname: Nicoletti
authors.affiliation: INSERM U1148, Laboratory of Vascular Translational Science, University Paris Diderot, University Paris 13, Sorbonne Paris Cité, X Bichat Hospital, and Département Hospitalo-Universitaire (DHU) FIRE, F-75018 Paris, France.
authors.forename: Didier
authors.initials: D
authors.lastname: Letourneur
authors.affiliation: EA 2496 Orofacial pathologies, imaging and biotherapies, Dental School Faculty, University Paris Descartes, and Life imaging Platform (PIV), Montrouge, France.|Department of Odontology, University Hospitals, AP-HP, Paris, France.
authors.forename: Marie-Laure
authors.initials: ML
authors.lastname: Colombier
authors.affiliation: Department of Mining and Materials Engineering, McGill University, Montreal, Quebec, Canada.
authors.forename: Showan N
authors.initials: SN
authors.lastname: Nazhat
authors.affiliation: EA 2496 Orofacial pathologies, imaging and biotherapies, Dental School Faculty, University Paris Descartes, and Life imaging Platform (PIV), Montrouge, France.|Department of Periodontology, Service of Odontology, Rothschild Hospital, AP-HP, University Denis Diderot, U.F.R. of Odontology, Paris, France.
authors.forename: Philippe
authors.initials: P
authors.lastname: Bouchard
authors.affiliation: EA 2496 Orofacial pathologies, imaging and biotherapies, Dental School Faculty, University Paris Descartes, and Life imaging Platform (PIV), Montrouge, France.|Department of Odontology, University Hospitals, AP-HP, Paris, France.
authors.forename: Catherine
authors.initials: C
authors.lastname: Chaussain
authors.affiliation: EA 2496 Orofacial pathologies, imaging and biotherapies, Dental School Faculty, University Paris Descartes, and Life imaging Platform (PIV), Montrouge, France.
authors.forename: Gael Y
authors.initials: GY
authors.lastname: Rochefort
mesh_terms: D000818:Animals; D001861:Bone Regeneration; D024042:Collagen Type I; D003782:Dental Pulp / Q000166:cytology*; D005782:Gels; D048091:Guided Tissue Regeneration / Q000379:methods*; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000166:cytology; D010012:Osteogenesis; D051381:Rats; D017208:Rats, Wistar; D012886:Skull / Q000000981:diagnostic imaging / Q000601:surgery*; D054457:Tissue Scaffolds; D055114:X-Ray Microtomography
chemical_list: D024042:Collagen Type I; D005782:Gels