title: Resonance Raman in Vitro Detection and Differentiation of the Nitrite-Induced Hemoglobin Adducts in Functional Human Red Blood Cells.
abstract: This work presents in vitro studies of the functional, isolated human red blood cells (RBCs) treated with various concentrations of Na14NO2and Na15NO2with the use of resonance Raman spectroscopy (RRS) at two different laser excitations supported by absorption spectrophotometry (UV-vis). The products of the reaction between oxyhemoglobin (oxyHb) in isolated RBCs with NaNO2were analyzed and identified in situ. The metHb-H2O was found to be the major product of this reaction; however, additional adducts were also clearly observed. Vibrational analysis allowed identification of various Hb3+NO2species (Fe3+-O-N=O with O-binding mode of nitrite ion to the Fe3+core and nitrovinyl adducts with 2-vinyl nitration favored over 4-vinyl nitration) as well as the Fe3+-NO adduct. In addition, we were able to visualize in situ the Hb-NO2species inside functional RBCs with the use of Raman imaging.
authors.affiliation: Jagiellonian Centre for Experimental Therapeutics (JCET), Jagiellonian University , Bobrzynskiego 14, Krakow 30-348, Poland.
authors.forename: Katarzyna M
authors.initials: KM
authors.lastname: Marzec
authors.affiliation: Jagiellonian Centre for Experimental Therapeutics (JCET), Jagiellonian University , Bobrzynskiego 14, Krakow 30-348, Poland.|Faculty of Chemistry, Jagiellonian University , Ingardena 3, Krakow 30-060, Poland.
authors.forename: Jakub
authors.initials: J
authors.lastname: Dybas
authors.affiliation: Jagiellonian Centre for Experimental Therapeutics (JCET), Jagiellonian University , Bobrzynskiego 14, Krakow 30-348, Poland.|Department of Experimental Pharmacology, Jagiellonian University Medical College , Grzegorzecka 16, Krakow 31-531, Poland.
authors.forename: Stefan
authors.initials: S
authors.lastname: Chlopicki
authors.affiliation: Jagiellonian Centre for Experimental Therapeutics (JCET), Jagiellonian University , Bobrzynskiego 14, Krakow 30-348, Poland.|Faculty of Chemistry, Jagiellonian University , Ingardena 3, Krakow 30-060, Poland.
authors.forename: Malgorzata
authors.initials: M
authors.lastname: Baranska
mesh_terms: D002454:Cell Differentiation; D004912:Erythrocytes / Q000737:chemistry* / Q000166:cytology / Q000378:metabolism; D006454:Hemoglobins / Q000737:chemistry* / Q000378:metabolism; D006801:Humans; D009573:Nitrites / Q000737:chemistry* / Q000378:metabolism; D013059:Spectrum Analysis, Raman
chemical_list: D006454:Hemoglobins; D009573:Nitrites