title: Methotrexate and Cytarabine-Loaded Nanocarriers for Multidrug Cancer Therapy. Spectroscopic Study.
abstract: Determining the properties of nanoparticles obtained by novel methods and defining the scope of their application as drug carriers has important practical significance. This article presents the pioneering studies concerning high degree incorporation of cytarabine (AraC) and methotrexate (MTX) into liposome vesicles. The main focus of this study were cytarabine-methotrexate-dipalmitoylphosphatidylcholine (DPPC) interactions observed in the gel and fluid phases of DPPC bilayers. The proposed new method of use the Transmittance2919/2850ratio presented in our research is sensitive to subtle changes in conformational order resulting from rotations, kinks and bends of the lipid chains. The transition temperatures characterized by Fourier Transform Infrared Spectroscopy (FT-IR) were consistent with the results obtained by Differential Scanning Calorimetry (DSC). Transmission Electron Microscopy (TEM) was used in order to determine the size and shape of the liposomes obtained. The mutual interactions occurring between the drugs studied and the phospholipids were analyzed using the Nuclear Magnetic Resonance (NMR).
authors.affiliation: Department of Materials Chemistry and Chemical Technology, Institute of Chemistry, University of Silesia, 40-006 Katowice, Poland. danuta.pentak@us.edu.pl.
authors.forename: Danuta
authors.initials: D
authors.lastname: Pentak
authors.affiliation: Department of Synthesis Chemistry, Institute of Chemistry, University of Silesia, 40-006 Katowice, Poland. violetta.kozik@us.edu.pl.
authors.forename: Violetta
authors.initials: V
authors.lastname: Kozik
authors.affiliation: Department of Organic Chemistry, Institute of Chemistry, University of Silesia, 40-006 Katowice, Poland. andrzej.bak@us.edu.pl.
authors.forename: Andrzej
authors.initials: A
authors.lastname: Bąk
authors.affiliation: Department of Organic Chemistry, Institute of Chemistry, University of Silesia, 40-006 Katowice, Poland. pdybal@us.edu.pl.
authors.forename: Paulina
authors.initials: P
authors.lastname: Dybał
authors.affiliation: Center for Translational Research and Molecular Biology of Cancer, Maria Skłodowska-Curie Memorial Cancer Center and Institute of Oncology, Gliwice Branch, 44-100 Gliwice, Poland. asochanik@gmail.com.
authors.forename: Aleksander
authors.initials: A
authors.lastname: Sochanik
authors.affiliation: Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Comenius University, 832 32 Bratislava, Slovakia. josef.jampilek@gmail.com.
authors.forename: Josef
authors.initials: J
authors.lastname: Jampilek
mesh_terms: D015060:1,2-Dipalmitoylphosphatidylcholine / Q000737:chemistry; D000971:Antineoplastic Combined Chemotherapy Protocols / Q000008:administration & dosage*; D002152:Calorimetry, Differential Scanning; D003561:Cytarabine / Q000008:administration & dosage*; D004337:Drug Carriers; D008727:Methotrexate / Q000008:administration & dosage*; D046529:Microscopy, Electron, Transmission; D053758:Nanoparticles; D009369:Neoplasms / Q000188:drug therapy*; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D004337:Drug Carriers; D003561:Cytarabine; D015060:1,2-Dipalmitoylphosphatidylcholine; D008727:Methotrexate
keywords: cytarabine; delivery systems; methotrexate