title: Simultaneous Determination of Four Tanshinones by UPLC-TQ/MS and Their Pharmacokinetic Application after Administration of Single Ethanol Extract of Danshen Combined with Water Extract in Normal and Adenine-Induced Chronic Renal Failure Rats.
abstract: Salvia miltiorrhiza, one of the major traditional Chinese medicines, is commonly used and the main active ingredients-tanshinones-possess the ability to improve renal function. In this paper, the UPLC-TQ/MS method of simultaneously determining four tanshinones-tanshinone IIA, dihydrotanshinone I, tanshinone I, and cryptotanshinone-was established and applied to assess the pharmacokinetics in normal and chronic renal failure (CRF) rat plasma. The pharmacokinetics of tanshinones in rats were studied after separately intragastric administration ofSalvia miltiorrhizaethanol extract (SMEE) (0.65 g/kg), SMEE (0.65 g/kg) combined withSalvia miltiorrhizawater extract (SMWE) (1.55 g/kg). The results showed Cmaxand AUC0-tof tanshinone IIA, tanshinone I, cryptotanshinone reduced by 50%~80% and CLz/F increased by 2~4 times (p< 0.05) in model group after administrated with SMEE. Nevertheless, after intragastric administration of a combination of SMWE and SMEE, the Cmaxand AUC0-tof four tanshinones were upregulated and CLz/F was downregulated, which undulated similarity from the model group to the normal group with compatibility of SMEE and SMWE. These results hinted that SMWE could improve the bioavailability of tanshinones in CRF rats, which provides scientific information for further exploration the mechanism of the combination of SMWE and SMEE and offers a reference for clinical administration ofSalvia miltiorrhiza.
authors.affiliation: Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, National and Local Collaborative Engineering Center of Chinese Medicinal Resources Industrialization and Formulae Innovative Medicine, and Jiangsu Key Laboratory for High Technology Research of TCM Formulae, Nanjing University of Chinese Medicine, Nanjing 210023, China. caihongdie@foxmail.com.
authors.forename: Hong-Die
authors.initials: HD
authors.lastname: Cai
authors.affiliation: Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, National and Local Collaborative Engineering Center of Chinese Medicinal Resources Industrialization and Formulae Innovative Medicine, and Jiangsu Key Laboratory for High Technology Research of TCM Formulae, Nanjing University of Chinese Medicine, Nanjing 210023, China. sushulan1974@njucm.edu.cn.
authors.forename: Shu-Lan
authors.initials: SL
authors.lastname: Su
authors.affiliation: Hainan Provincial Key Laboratory of R&D of Tropical Herbs, School of Pharmacy, Hainan Medical University, Haikou 571199, China. lyhssl@126.com.
authors.forename: Yonghui
authors.initials: Y
authors.lastname: Li
authors.affiliation: Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, National and Local Collaborative Engineering Center of Chinese Medicinal Resources Industrialization and Formulae Innovative Medicine, and Jiangsu Key Laboratory for High Technology Research of TCM Formulae, Nanjing University of Chinese Medicine, Nanjing 210023, China. 04040416@163.com.
authors.forename: Zhenhua
authors.initials: Z
authors.lastname: Zhu
authors.affiliation: Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, National and Local Collaborative Engineering Center of Chinese Medicinal Resources Industrialization and Formulae Innovative Medicine, and Jiangsu Key Laboratory for High Technology Research of TCM Formulae, Nanjing University of Chinese Medicine, Nanjing 210023, China. guojianming@163.com.
authors.forename: Jianming
authors.initials: J
authors.lastname: Guo
authors.affiliation: Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, National and Local Collaborative Engineering Center of Chinese Medicinal Resources Industrialization and Formulae Innovative Medicine, and Jiangsu Key Laboratory for High Technology Research of TCM Formulae, Nanjing University of Chinese Medicine, Nanjing 210023, China. zhuyue@163.com.
authors.forename: Yue
authors.initials: Y
authors.lastname: Zhu
authors.affiliation: Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, National and Local Collaborative Engineering Center of Chinese Medicinal Resources Industrialization and Formulae Innovative Medicine, and Jiangsu Key Laboratory for High Technology Research of TCM Formulae, Nanjing University of Chinese Medicine, Nanjing 210023, China. gsh916@163.com.
authors.forename: Sheng
authors.initials: S
authors.lastname: Guo
authors.affiliation: Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, National and Local Collaborative Engineering Center of Chinese Medicinal Resources Industrialization and Formulae Innovative Medicine, and Jiangsu Key Laboratory for High Technology Research of TCM Formulae, Nanjing University of Chinese Medicine, Nanjing 210023, China. qiandwnj@126.com.
authors.forename: Dawei
authors.initials: D
authors.lastname: Qian
authors.affiliation: Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, National and Local Collaborative Engineering Center of Chinese Medicinal Resources Industrialization and Formulae Innovative Medicine, and Jiangsu Key Laboratory for High Technology Research of TCM Formulae, Nanjing University of Chinese Medicine, Nanjing 210023, China. duanja@163.com.
authors.forename: Jinao
authors.initials: J
authors.lastname: Duan
mesh_terms: D045784:Abietanes / Q000493:pharmacokinetics* / Q000494:pharmacology; D000818:Animals; D002853:Chromatography, Liquid / Q000379:methods*; D007676:Kidney Failure, Chronic / Q000188:drug therapy; D013058:Mass Spectrometry / Q000379:methods*; D010616:Phenanthrenes / Q000493:pharmacokinetics* / Q000494:pharmacology; D010936:Plant Extracts / Q000493:pharmacokinetics / Q000494:pharmacology; D051381:Rats; D027561:Salvia miltiorrhiza / Q000737:chemistry*
chemical_list: D045784:Abietanes; D010616:Phenanthrenes; D010936:Plant Extracts; C021751:tanshinone; C037886:cryptotanshinone
keywords: Salvia miltiorrhiza Bge.; UPLC-TQ/MS; chronic renal failure; pharmacokinetics; tanshinones