title: Long non-coding RNA XIST promotes cell growth and invasion through regulating miR-497/MACC1 axis in gastric cancer.
abstract: Abnormal expression of long non-coding RNA (lncRNAs) often contributes to unrestricted growth and invasion of cancer cells. LncRNA XIST expression is up-regulated in several cancers, however, its modulatory mechanism in gastric cancer (GC) has not been elucidated. In the present study, we found that XIST expression was significantly increased in GC tissues and cell lines. LncRNA XIST promoted cell cycle progression from the G1 phase to the S phase and protected cells from apoptosis, which contributed to GC cell growth. LncRNA XIST also contributed to GC cell invasion both in vitro and in vivo. We revealed that XIST functioned as competing endogenous RNA to repress miR-497, which controlled its down-stream target MACC1. We proposed that XIST was responsible for GC cell proliferation and invasion and XIST exerted its function through the miR-497/MACC1 axis. Our findings suggested that lncRNA XIST may be a candidate prognostic biomarker and a target for new therapies in GC patients.
authors.affiliation: Cancer Hospital of Guangzhou Medical University, Guangzhou, China.|Department of Gastroenterology, Guangzhou Medical University, Guangzhou, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Ma
authors.affiliation: Department of Gastroenterology, The First Hospital of Guangzhou, Guangzhou, China.
authors.forename: Yongjian
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: Cancer Center, TCM-Integrated Hospital, Southern Medical University, Guangzhou, China.
authors.forename: Xiaojun
authors.initials: X
authors.lastname: Luo
authors.affiliation: Xiamen Hospital of Traditional Chinese Medicine, Xiamen, China.|Xiamen Hospital Affiliated to Fujian University of Traditional Chinese Medicine, Xiamen, China.
authors.forename: Hai
authors.initials: H
authors.lastname: Gao
authors.affiliation: Cancer Hospital of Guangzhou Medical University, Guangzhou, China.
authors.forename: Xubin
authors.initials: X
authors.lastname: Deng
authors.affiliation: Department of Gastroenterology, The First Hospital of Guangzhou, Guangzhou, China.
authors.forename: Yingjie
authors.initials: Y
authors.lastname: Jiang
mesh_terms: D000818:Animals; D002453:Cell Cycle; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D051379:Mice; D035683:MicroRNAs / Q000235:genetics*; D009361:Neoplasm Invasiveness; D009368:Neoplasm Transplantation; D062085:RNA, Long Noncoding / Q000235:genetics*; D013274:Stomach Neoplasms / Q000235:genetics* / Q000473:pathology*; D015534:Trans-Activators; D014157:Transcription Factors / Q000235:genetics*; D047368:Tumor Burden; D015854:Up-Regulation
chemical_list: C544162:MACC1 protein, human; C559640:MIRN497 microRNA, human; D035683:MicroRNAs; D062085:RNA, Long Noncoding; D015534:Trans-Activators; D014157:Transcription Factors; C077885:XIST non-coding RNA
keywords: MACC1; gastric cancer; lncRNA XIST; miR-497