title: Isolation of Mouse Embryonic Stem Cell Lines in the Study of ERK1/2 MAP Kinase Signaling.
abstract: Mouse embryonic stem (ES) cells have proven to be invaluable research tools for dissecting the role of signaling pathways in embryonic development, adult physiology, and various diseases. ES cells are amenable to genetic manipulation by classical gene targeting via homologous recombination or by genome editing technologies. These cells can be used to generate genetically modified mouse models or to study the signaling circuitry regulating self-renewal and early lineage commitment. In this chapter, we describe methods used for the isolation and establishment of mouse ES cell lines from blastocyst embryos and for the measurement of ERK1/2 activity in ES cells.
authors.affiliation: Institute for Research in Immunology and Cancer, Université de Montréal, 2950, Chemin de Polytechnique, Montréal, QC, Canada, H3C 3J7. marc.k.saba.el.leil@umontreal.ca.
authors.forename: Marc K
authors.initials: MK
authors.lastname: Saba-El-Leil
authors.affiliation: Institute for Research in Cancer of Montpellier, 208 Rue des Apothicaires, 34298, Montpellier Cedex 5, France.
authors.forename: Christophe
authors.initials: C
authors.lastname: Frémin
authors.affiliation: Institute for Research in Immunology and Cancer, Université de Montréal, 2950, Chemin de Polytechnique, Montréal, QC, Canada, H3C 3J7. sylvain.meloche@umontreal.ca.|Molecular Biology Program, Université de Montréal, Montreal, QC, Canada, H3T 1J4. sylvain.meloche@umontreal.ca.|Department of Pharmacology, Université de Montréal, Montreal, QC, Canada, H3T 1J4. sylvain.meloche@umontreal.ca.
authors.forename: Sylvain
authors.initials: S
authors.lastname: Meloche
mesh_terms: D000818:Animals; D001755:Blastocyst / Q000378:metabolism; D015153:Blotting, Western; D002454:Cell Differentiation; D002460:Cell Line; D000066673:Cell Self Renewal; D004789:Enzyme Activation; D055786:Gene Knockout Techniques; D051379:Mice; D019950:Mitogen-Activated Protein Kinase 1 / Q000378:metabolism*; D048052:Mitogen-Activated Protein Kinase 3 / Q000378:metabolism*; D000066450:Mouse Embryonic Stem Cells / Q000378:metabolism*; D015398:Signal Transduction
chemical_list: D019950:Mitogen-Activated Protein Kinase 1; D048052:Mitogen-Activated Protein Kinase 3
keywords: Blastocyst isolation; ERK1/2 MAP kinase signaling; Mouse ES cells; Pluripotency; Self-renewal