title: Plant extracts: initial screening, identification of bioactive compounds and effect against Candida albicans biofilms.
abstract: This study screened plants from Brazilian Pantanal for Candida albicans antibiofilm activity.
  
  
  
  Sixty extracts were obtained from ten plants using different extraction methods. Antifungal activity was assessed. Effects on biofilm inhibition and disruption and cytotoxicity were also evaluated. The most active extract was chemically characterized.
  
  
  
  Buchenavia tomentosa ethanolic extract showed noticeable antifungal activity and was selected for biofilm experiments. Subinhibitory concentration of extract inhibited fungal adhesion. Maximum killing reached 90% of C. albicans cells in suspension and 65% of cells in biofilms. The active extract was noncytotoxic. Chemical characterization showed the presence of phenols. Ellagic and gallic acids showed activity on C. albicans.
  
  
  
  B. tomentosa extract and its isolated compound, ellagic acid, presented antibiofilm activity and low toxicity.
authors.affiliation: Faculty of Dentistry, Univ. Estadual Paulista/UNESP, Araraquara, R. Humaitá, 1680, Araraquara, SP 14801-903, Brazil.
authors.forename: Fernanda Lourenção
authors.initials: FL
authors.lastname: Brighenti
authors.affiliation: Institute of Biology, University of Campinas/UNICAMP, Postal Box 6109, Campinas, SP 3083-970, Brazil.
authors.forename: Marcos José
authors.initials: MJ
authors.lastname: Salvador
authors.affiliation: Institute of Biology, University of Campinas/UNICAMP, Postal Box 6109, Campinas, SP 3083-970, Brazil.
authors.forename: Aline Vidal Lacerda
authors.initials: AVL
authors.lastname: Gontijo
authors.affiliation: Faculty of Dentistry, Univ. Estadual Paulista/UNESP, Araçatuba, R. José Bonifácio, 1193, Araçatuba, SP 16015-050, Brazil.
authors.forename: Alberto Carlos Botazzo
authors.initials: ACB
authors.lastname: Delbem
authors.affiliation: Faculty of Dentistry, Univ. Estadual Paulista/UNESP, Araçatuba, R. José Bonifácio, 1193, Araçatuba, SP 16015-050, Brazil.
authors.forename: Ádina Cléia Botazzo
authors.initials: ÁCB
authors.lastname: Delbem
authors.affiliation: Universidade do Vale do Paraíba - UNIVAP, Av. Shishima Hifumi, 2911, São José dos Campos, SP 12244-390, Brazil.
authors.forename: Cristina Pacheco
authors.initials: CP
authors.lastname: Soares
authors.affiliation: Institute of Science & Technology, Univ. Estadual Paulista/UNESP, São José dos Campos, Av. Eng. Francisco José Longo, 777, São José dos Campos, SP 12245-000, Brazil.
authors.forename: Maria Alcionéiac Carvalho
authors.initials: MAC
authors.lastname: de Oliveira
authors.affiliation: Institute of Science & Technology, Univ. Estadual Paulista/UNESP, São José dos Campos, Av. Eng. Francisco José Longo, 777, São José dos Campos, SP 12245-000, Brazil.
authors.forename: Camila Miorelli
authors.initials: CM
authors.lastname: Girondi
authors.affiliation: Institute of Science & Technology, Univ. Estadual Paulista/UNESP, São José dos Campos, Av. Eng. Francisco José Longo, 777, São José dos Campos, SP 12245-000, Brazil.
authors.forename: Cristiane Yumi
authors.initials: CY
authors.lastname: Koga-Ito
mesh_terms: D000935:Antifungal Agents / Q000494:pharmacology*; D018441:Biofilms / Q000187:drug effects*; D001938:Brazil; D018938:Caco-2 Cells; D002176:Candida albicans / Q000187:drug effects*; D029764:Combretaceae / Q000737:chemistry; D004610:Ellagic Acid / Q000494:pharmacology; D005707:Gallic Acid / Q000494:pharmacology; D006801:Humans; D010636:Phenols / Q000494:pharmacology; D010936:Plant Extracts / Q000494:pharmacology*
chemical_list: D000935:Antifungal Agents; D010636:Phenols; D010936:Plant Extracts; D004610:Ellagic Acid; D005707:Gallic Acid
keywords: Candida albicans; antifungal agents; bioactive compounds; biofilms