title: Targeting Conserved Genes in Fusarium Species.
abstract: Fumonisins are important mycotoxins contaminating foods and feeds which are mainly produced by F. verticillioides and F. proliferatum. Additionally, both are pathogens of maize and other cereals. We describe two highly sensitive, rapid, and species-specific PCR protocols which enable detection and discrimination of these closely related species in cereal flour or grain samples. The specific primer pairs of these assays were based on the intergenic spacer region of the multicopy rDNA unit which highly improves the sensitivity of the PCR assay in comparison with single-copy target regions.
authors.affiliation: Facultad de Ciencias Biologicas, Departamento de Microbiologia, Universidad Complutense de Madrid, Jose Antonio Novais 12, Madrid, 28040, Spain.
authors.forename: Jéssica
authors.initials: J
authors.lastname: Gil-Serna
authors.affiliation: Facultad de Ciencias Biologicas, Departamento de Microbiologia, Universidad Complutense de Madrid, Jose Antonio Novais 12, Madrid, 28040, Spain.
authors.forename: Belén
authors.initials: B
authors.lastname: Patiño
authors.affiliation: Facultad de Ciencias Biologicas, Departamento de Genetica, Universidad Complutense de Madrid, Jose Antonio Novais 12, Madrid, 28040, Spain.
authors.forename: Miguel
authors.initials: M
authors.lastname: Jurado
authors.affiliation: Facultad de Ciencias Biologicas, Departamento de Genetica, Universidad Complutense de Madrid, Jose Antonio Novais 12, Madrid, 28040, Spain.
authors.forename: Salvador
authors.initials: S
authors.lastname: Mirete
authors.affiliation: Facultad de Ciencias Biologicas, Departamento de Microbiologia, Universidad Complutense de Madrid, Jose Antonio Novais 12, Madrid, 28040, Spain.
authors.forename: Covadonga
authors.initials: C
authors.lastname: Vázquez
authors.affiliation: Facultad de Ciencias Biologicas, Departamento de Genetica, Universidad Complutense de Madrid, Jose Antonio Novais 12, Madrid, 28040, Spain. tegonja@ucm.es.
authors.forename: M Teresa
authors.initials: MT
authors.lastname: González-Jaén
mesh_terms: D017124:Conserved Sequence; D021903:DNA, Ribosomal Spacer / Q000235:genetics; D019143:Evolution, Molecular; D005670:Fusarium / Q000145:classification* / Q000235:genetics*; D005800:Genes, Fungal; D016133:Polymerase Chain Reaction
chemical_list: D021903:DNA, Ribosomal Spacer
keywords: Detection; Fumonisins; Fusarium proliferatum; Fusarium verticillioides; Polymerase chain reaction (PCR); rDNA intergenic spacer (IGS)