title: Exopolysaccharide production inAnabaenasp. PCC 7120 under different CaCl2regimes.
abstract: Influence of various levels of CaCl2(0, 1, 10 and 100 mM) on exopolysaccharide production has been investigated in the cyanobacteriumAnabaena7120. At the concentration of 1 mM CaCl2, growth was found to be stimulatory while 100 mM was sub lethal for the cyanobacterial cells. Estimation of EPS content revealed that EPS production depends on the concentration of calcium ions in the immediate environment with maximum being at10 mM CaCl2. A possible involvement ofalr2882gene in the process of EPS production was also revealed through qRT-PCR. Further, FTIR-spectra marked the presence of aliphatic alkyl-group, primary amine-group, and polysaccharides along with shift in major absorption peaks suggesting that calcium levels in the external environment regulate the composition of EPS produced byAnabaena7120. Thus, both quantity and composition of EPS is affected under different calcium chloride concentrations presenting possibilities of EPS with novel unexplored features that may offer biotechnological applications.
authors.affiliation: Laboratory of Microbial Genetics, Department of Botany, Banaras Hindu University, Varanasi, 221005 India.
authors.forename: Savita
authors.initials: S
authors.lastname: Singh
authors.affiliation: Laboratory of Microbial Genetics, Department of Botany, Banaras Hindu University, Varanasi, 221005 India.
authors.forename: Ekta
authors.initials: E
authors.lastname: Verma
authors.affiliation: Laboratory of Microbial Genetics, Department of Botany, Banaras Hindu University, Varanasi, 221005 India.
authors.lastname: Niveshika
authors.affiliation: Laboratory of Microbial Genetics, Department of Botany, Banaras Hindu University, Varanasi, 221005 India.
authors.forename: Balkrishna
authors.initials: B
authors.lastname: Tiwari
authors.affiliation: Laboratory of Microbial Genetics, Department of Botany, Banaras Hindu University, Varanasi, 221005 India.
authors.forename: Arun Kumar
authors.initials: AK
authors.lastname: Mishra
keywords: Anabaena 7120; Extracellular polymeric substances; Fourier transform infra red spectroscopy; Quantitative reverse transcriptase-polymerase chain reaction; Scanning electron microscopy; alr2882 gene