title: Ultrastructural abnormalities and loss of myelinated fibers in the corpus callosum of demyelinated mice induced by cuprizone.
abstract: It is now accepted that white matter abnormalities play an important role in demyelinating diseases and a wide range of psychiatric disorders. Experimental demyelination (especially induced by cuprizone) has been investigated extensively. However, details regarding demyelination and ultrastructural changes of myelinated fibers have not been previously reported. Therefore, we determined the extent of demyelination using quantitative stereology. Mice exposed to cuprizone in the current study showed abnormal anxiety-like behavior without impaired spatial learning or memory. The myelinated fibers in whole corpus callosum of mice exposed to cuprizone showed extensive myelin deficiencies and occasional axonal injuries. The total length of the myelinated fibers in whole corpus callosum of mice exposed to cuprizone was significantly decreased by 45% compared with control mice. The loss of myelinated fibers was mainly due to the marked loss of the fibers with a diameter of 0.4 to 0.8 μm. The g-ratio of the myelinated fibers in the corpus callosum of mice exposed to cuprizone (0.69 ± 0.02) was significantly decreased compared with control mice (0.76 ± 0.02). These results might help us to further understand the role of white matter abnormalities in demyelinating diseases or a wide range of psychiatric disorders. © 2016 Wiley Periodicals, Inc.
authors.affiliation: Institute of Life Science, Chongqing Medical University, Chongqing, People's Republic of China.|Department of Histology and Embryology, Faculty of Basic Medical Sciences, Chongqing Medical University, Chongqing, People's Republic of China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Xiu
authors.affiliation: Department of Histology and Embryology, Faculty of Basic Medical Sciences, Chongqing Medical University, Chongqing, People's Republic of China.
authors.forename: Guo-Hua
authors.initials: GH
authors.lastname: Cheng
authors.affiliation: Department of Histology and Embryology, Faculty of Basic Medical Sciences, Chongqing Medical University, Chongqing, People's Republic of China.
authors.forename: Chao
authors.initials: C
authors.lastname: Peng
authors.affiliation: Department of Histology and Embryology, Faculty of Basic Medical Sciences, Chongqing Medical University, Chongqing, People's Republic of China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Histology and Embryology, Faculty of Basic Medical Sciences, Chongqing Medical University, Chongqing, People's Republic of China.
authors.forename: Yong-de
authors.initials: YD
authors.lastname: Li
authors.affiliation: Department of Histology and Embryology, Faculty of Basic Medical Sciences, Chongqing Medical University, Chongqing, People's Republic of China.
authors.forename: Feng-Lei
authors.initials: FL
authors.lastname: Chao
authors.affiliation: Department of Histology and Embryology, Faculty of Basic Medical Sciences, Chongqing Medical University, Chongqing, People's Republic of China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Tang
mesh_terms: D000818:Animals; D002614:Chelating Agents / Q000633:toxicity; D003337:Corpus Callosum / Q000187:drug effects / Q000473:pathology* / Q000648:ultrastructure*; D003471:Cuprizone / Q000633:toxicity; D003711:Demyelinating Diseases / Q000139:chemically induced* / Q000473:pathology*; D004195:Disease Models, Animal; D005106:Exploratory Behavior / Q000187:drug effects; D008297:Male; D018782:Maze Learning / Q000187:drug effects; D051379:Mice; D008810:Mice, Inbred C57BL; D046529:Microscopy, Electron, Transmission; D004676:Myelin Basic Protein / Q000378:metabolism; D009413:Nerve Fibers, Myelinated / Q000473:pathology / Q000648:ultrastructure; D045442:Rotarod Performance Test
chemical_list: D002614:Chelating Agents; D004676:Myelin Basic Protein; D003471:Cuprizone
keywords: RRID: AB_1841021; corpus callosum; cuprizone; demyelination; stereology