title: Coriandrum sativum and Lavandula angustifolia Essential Oils: Chemical Composition and Activity on Central Nervous System.
abstract: The aims of this study are to determine the chemical composition ofLavandula angustifoliaMill. andCoriandrum sativumL. essential oils, to evaluate their cytotoxic effects in SH-SY5Y human neuroblastoma cells, to investigate whether an alteration of adenylate cyclase 1 (ADCY1) and of extracellular signal-regulated kinase (ERK) expression can take part in the molecular mechanisms of the essential oils, and to study their possible neuronal electrophysiological effects. The essential oils were obtained by hydrodistillation, and studied by GC and GC-MS. In the oils fromL. angustifoliaandC. sativum, linalool was the main component (33.1% and 67.8%, respectively). SH-SY5Y cells were incubated with different concentrations of essential oils and of linalool. Cell viability and effects on ADCY1 and ERK expression were analyzed using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide MTT and Western blotting, respectively. Variation in cellular electrophysiology was studied in primary cultures of rat cortical neurons with a multi-electrode array (MEA)-based approach. The essential oils and linalool revealed different cytotoxic activities. Linalool inhibited ADCY1 and ERK expression. Neuronal networks subjected toL. angustifoliaandC. sativumessential oils showed a concentration-dependent inhibition of spontaneous electrical activity.
authors.affiliation: Department of Pharmacy, University of Salerno, Via Giovanni Paolo II, 132, 84084 Fisciano (Salerno), Italy. lcaputo@unisa.it.
authors.forename: Lucia
authors.initials: L
authors.lastname: Caputo
authors.affiliation: Department of Pharmacy, University of Salerno, Via Giovanni Paolo II, 132, 84084 Fisciano (Salerno), Italy. luceia.souza@ufrgs.br.|Department of Agronomy, University of Rio Grande do Sul (UFRGS), 91501-970 Porto Alegre, Brazil. luceia.souza@ufrgs.br.
authors.forename: Lucéia Fátima
authors.initials: LF
authors.lastname: Souza
authors.affiliation: ETT Spa, via Sestri 37, 16154 Genova, Italy. susanna.alloisio@ettsolutions.com.
authors.forename: Susanna
authors.initials: S
authors.lastname: Alloisio
authors.affiliation: Dipartimento di Scienze della Terra, dell'Ambiente e della Vita, University of Genoa, Corso Europa 26, 16132 Genoa, Italy. cornara@dipteris.unige.it.
authors.forename: Laura
authors.initials: L
authors.lastname: Cornara
authors.affiliation: Department of Pharmacy, University of Salerno, Via Giovanni Paolo II, 132, 84084 Fisciano (Salerno), Italy. defeo@unisa.it.
authors.forename: Vincenzo
authors.initials: V
authors.lastname: De Feo
mesh_terms: D000262:Adenylyl Cyclases / Q000235:genetics / Q000378:metabolism; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D002490:Central Nervous System / Q000187:drug effects / Q000378:metabolism; D028526:Coriandrum / Q000737:chemistry*; D048049:Extracellular Signal-Regulated MAP Kinases / Q000235:genetics / Q000378:metabolism; D006801:Humans; D027523:Lavandula / Q000737:chemistry*; D009822:Oils, Volatile / Q000737:chemistry* / Q000494:pharmacology*
chemical_list: D009822:Oils, Volatile; D048049:Extracellular Signal-Regulated MAP Kinases; D000262:Adenylyl Cyclases; C106670:adenylyl cyclase 1
keywords: Adenylyl Cyclase (ADCY); Central Nervous System (CNS); Coriandrum sativum; Extracellular Signal-Regulated Kinase (ERK); Lavandula angustifolia; Multi Electrode Array (MEA); cytotoxicity; essential oil; linalool