title: A mitochondria-targeted fluorescence probe for ratiometric detection of endogenous hypochlorite in the living cells.
abstract: A mitochondria-targeted fluorescence probe (CPBT) for ratiometric detection of endogenous hypochlorite in the living cells was developed. CPBT could detect hypochlorite with high selectivity and sensitivity in a ratiometric manner based on FRET mechanism. In absence of hypochlorite, when CPBT was excited with absorption maximum wavelength of the donor moiety, it showed the emission of acceptor moiety because of FRET process. However, in the presence of hypochlorite, the reaction of CC double bond with hypochlorite interrupted the conjugation system resulting in the inhibition of FRET process and the emission of the donor moiety. The two well-resolved emission bands can ensure accurate detection of hypochlorite. A good linear relationship between the fluorescence intensity ratios of the two emissions and the ClO-concentrations in the range from 41.8 nM (detection limit) to 12.5 μM was established. Importantly, CPBT could localize mainly in the mitochondria of RAW264.7 cells. CPBT was successfully used to fluorescence ratiometric imaging of endogenous hypochlorite in RAW264.7 cells.
authors.affiliation: Institute of Organic Chemistry, School of Chemistry and Chemical Engineering, Shandong University, Jinan 250100, PR China.
authors.forename: Wen-Li
authors.initials: WL
authors.lastname: Wu
authors.affiliation: Institute of Developmental Biology, School of Life Science, Shandong University, Jinan 250100, PR China.
authors.forename: Xuan
authors.initials: X
authors.lastname: Zhao
authors.affiliation: Institute of Organic Chemistry, School of Chemistry and Chemical Engineering, Shandong University, Jinan 250100, PR China.
authors.forename: Long-Long
authors.initials: LL
authors.lastname: Xi
authors.affiliation: Institute of Organic Chemistry, School of Chemistry and Chemical Engineering, Shandong University, Jinan 250100, PR China.
authors.forename: Miao-Fei
authors.initials: MF
authors.lastname: Huang
authors.affiliation: Institute of Organic Chemistry, School of Chemistry and Chemical Engineering, Shandong University, Jinan 250100, PR China.
authors.forename: Wen-Hui
authors.initials: WH
authors.lastname: Zeng
authors.affiliation: Institute of Developmental Biology, School of Life Science, Shandong University, Jinan 250100, PR China. Electronic address: miaojy@sdu.edu.cn.
authors.forename: Jun-Ying
authors.initials: JY
authors.lastname: Miao
authors.affiliation: Institute of Organic Chemistry, School of Chemistry and Chemical Engineering, Shandong University, Jinan 250100, PR China. Electronic address: bxzhao@sdu.edu.cn.
authors.forename: Bao-Xiang
authors.initials: BX
authors.lastname: Zhao
mesh_terms: D000818:Animals; D031541:Fluorescence Resonance Energy Transfer; D005456:Fluorescent Dyes; D006997:Hypochlorous Acid / Q000032:analysis*; D051379:Mice; D008856:Microscopy, Fluorescence; D008928:Mitochondria / Q000737:chemistry*; D000067996:RAW 264.7 Cells
chemical_list: D005456:Fluorescent Dyes; D006997:Hypochlorous Acid
keywords: Endogenous hypochlorite; Fluorescence; Mitochondria-target; Ratiometric