title: Relationship between nanotopographical alignment and stem cell fate with live imaging and shape analysis.
abstract: The topography of a biomaterial regulates cellular interactions and determine stem cell fate. A complete understanding of how topographical properties affect cell behavior will allow the rational design of material surfaces that elicit specified biological functions once placed in the body. To this end, we fabricate substrates with aligned or randomly organized fibrous nanostructured topographies. Culturing adipose-derived stem cells (ASCs), we explore the dynamic relationship between the alignment of topography, cell shape and cell differentiation to osteogenic and myogenic lineages. We show aligned topographies differentiate cells towards a satellite cell muscle progenitor state - a distinct cell myogenic lineage responsible for postnatal growth and repair of muscle. We analyze cell shape between the different topographies, using fluorescent time-lapse imaging over 21 days. In contrast to previous work, this allows the direct measurement of cell shape at a given time rather than defining the morphology of the underlying topography and neglecting cell shape. We report quantitative metrics of the time-based morphological behaviors of cell shape in response to differing topographies. This analysis offers insights into the relationship between topography, cell shape and cell differentiation. Cells differentiating towards a myogenic fate on aligned topographies adopt a characteristic elongated shape as well as the alignment of cells.
authors.affiliation: Biomaterials and Tissue Engineering Research Unit, School of Aeronautical Mechanical and Mechatronics Engineering, University of Sydney, Sydney, NSW, 2006, Australia.
authors.forename: Peter
authors.initials: P
authors.lastname: Newman
authors.affiliation: EMBL Australia node in Single Molecule Science, School of Medical Sciences, The University of New South Wales, Sydney, Australia.
authors.forename: Jorge Luis
authors.initials: JL
authors.lastname: Galenano Niño
authors.affiliation: Department of Biomedical Engineering, School of Biomedical Engineering, Science and Health Systems, Drexel University, Philadelphia, PA 19104, USA.
authors.forename: Pamela
authors.initials: P
authors.lastname: Graney
authors.affiliation: Institute for Frontier Materials, Deakin University, Geelong, Victoria, 3216, Australia.
authors.forename: Joselito M
authors.initials: JM
authors.lastname: Razal
authors.affiliation: Laboratory for Sustainable Technology, Department of Chemical and Biomolecular Engineering, University of Sydney, NSW, 2006, Australia.|Australian Institute for Nanoscale Science and Technology, University of Sydney, NSW, 2006, Australia.
authors.forename: Andrew I
authors.initials: AI
authors.lastname: Minett
authors.affiliation: Biomaterials Innovation Research Center, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Cambridge, Massachusetts 02139, USA.
authors.forename: João
authors.initials: J
authors.lastname: Ribas
authors.affiliation: Nano-Science &Technology Center, LINTEC of America Inc., Richardson, Texas 75081, USA.
authors.forename: Raquel
authors.initials: R
authors.lastname: Ovalle-Robles
authors.affiliation: EMBL Australia node in Single Molecule Science, School of Medical Sciences, The University of New South Wales, Sydney, Australia.|Sydney Medical School, The University of Sydney, NSW, 2006, Australia.
authors.forename: Maté
authors.initials: M
authors.lastname: Biro
authors.affiliation: Biomaterials and Tissue Engineering Research Unit, School of Aeronautical Mechanical and Mechatronics Engineering, University of Sydney, Sydney, NSW, 2006, Australia.
authors.forename: Hala
authors.initials: H
authors.lastname: Zreiqat
mesh_terms: D017667:Adipocytes / Q000166:cytology; D001672:Biocompatible Materials; D002448:Cell Adhesion; D002452:Cell Count; D002454:Cell Differentiation; D019070:Cell Lineage; D048430:Cell Shape; D004548:Elasticity; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D024510:Muscle Development; D010012:Osteogenesis; D013234:Stem Cells / Q000166:cytology*; D059008:Time-Lapse Imaging
chemical_list: D001672:Biocompatible Materials