title: [Cytotoxicity of natural anti-HLA antibodies in Moroccan patients awaiting for kidney transplantation].
abstract: The presence of anti-HLA antibodies in the serum of a patient result from an immune response produced during an immunizing event as transfusion, pregnancy or graft. These antibodies can be cytotoxic by activating the complement pathway via C1q and may cause organ rejection during the transplant. Some male patients awaiting kidney transplantation are seropositive for anti-HLA antibodies when they have no immunizing antecedent event. These antibodies are qualified as natural antibodies. Our work is to assess the cytotoxicity of natural anti-HLA antibodies in patients followed at the immunology laboratory of the blood transfusion service and hemovigilance (STSH) as part of the kidney transplant.
  
  
  METHODS
  We evaluated the cytotoxicity of HLA antibodies detected in male Moroccan patients without immunization history using C1qScreen One Lambda reagent for Luminex™.
  
  
  RESULTS
  Non-immunized men were positive for HLA antibodies screening in 25.4%. These antibodies are not cytotoxic.
  
  
  CONCLUSIONS
  Our study showed a positivity rate of natural HLA antibody low than the literature (25.4% against 63%). It appears that these natural antibodies are not cytotoxic and their involvement in renal transplant remains to be determined.
authors.affiliation: Unité d'immunologie, service de transfusion sanguine et d'hémovigilance du CHU Ibn Sina, rue Lamfadel Cherkaoui, BP 6527, Rabat, Maroc; UPR d'immunologie, faculté de médecine et de pharmacie, université Mohamed V, avenue Mohamed Belarbi El Alaoui, BP 6203, Rabat, Maroc. Electronic address: benseffajnadia@yahoo.fr.
authors.forename: Nadia
authors.initials: N
authors.lastname: Benseffaj
authors.affiliation: Unité d'immunologie, service de transfusion sanguine et d'hémovigilance du CHU Ibn Sina, rue Lamfadel Cherkaoui, BP 6527, Rabat, Maroc.
authors.forename: Sanae
authors.initials: S
authors.lastname: Ouadghiri
authors.affiliation: Unité d'immunologie, service de transfusion sanguine et d'hémovigilance du CHU Ibn Sina, rue Lamfadel Cherkaoui, BP 6527, Rabat, Maroc.
authors.forename: Asmaa Drissi
authors.initials: AD
authors.lastname: Bourhanbour
authors.affiliation: UPR d'immunologie, faculté de médecine et de pharmacie, université Mohamed V, avenue Mohamed Belarbi El Alaoui, BP 6203, Rabat, Maroc.
authors.forename: Asmae Noor
authors.initials: AN
authors.lastname: Zerrouki
authors.affiliation: Unité d'immunologie, service de transfusion sanguine et d'hémovigilance du CHU Ibn Sina, rue Lamfadel Cherkaoui, BP 6527, Rabat, Maroc; UPR d'immunologie, faculté de médecine et de pharmacie, université Mohamed V, avenue Mohamed Belarbi El Alaoui, BP 6203, Rabat, Maroc.
authors.forename: Malika
authors.initials: M
authors.lastname: Essakalli
mesh_terms: D006680:HLA Antigens / Q000276:immunology*; D006650:Histocompatibility Testing / Q000295:instrumentation; D006801:Humans; D007518:Isoantibodies / Q000097:blood*; D016030:Kidney Transplantation; D008297:Male; D009018:Morocco; D012698:Serologic Tests / Q000295:instrumentation
chemical_list: D006680:HLA Antigens; D007518:Isoantibodies
keywords: Anti-HLA antibodies; Anticorps anti-HLA; Anticorps naturels; C1q; Cytotoxicity; Cytotoxicité; Luminex™; Male; Natural antibodies; Sexe masculin