title: Developmental validation of the HomyGene19+14Y System.
abstract: The HomyGene19+14Y System (HG19+14Y) is a PCR-based amplification kit that enables typing of 18 autosomal short tandem repeat (STR) loci (i.e., CSF1PO, D2S1338, D3S1358, D5S818, D6S1043, D7S820, D8S1179, D12S391, D13S317, D16S539, D18S51, D19S433, D21S11, FGA, Penta E, TPOX, TH01, vWA), 14 widely used Y chromosome STR (Y-STR) loci (Y_GATA_H4, DYS385a/b, DYS389I/II, DYS390, DYS391, DYS392, DYS393, DYS438, DYS439, DYS456, DYS458, DYS635), and amelogenin. This multiplex system was designed for the simultaneous analysis of amelogenin-Y allele mutation, single-source searches, kinship (including familial searching), mixture profiles, international data sharing, and other forensic applications. In this study, the multiplex system was validated for sensitivity, specificity, DNA mixtures, stability, precision, stutter, reproducibility, parallel tests, PCR-based conditions, and population analysis according to the Scientific Working Group on DNA Analysis Methods (SWGDAM) developmental validation guidelines. A total of 212 alleles were detected for the 18 autosomal STR loci among 528 Guangdong Han individuals, and 431 haplotypes were found for 14 Y-STRs among 452 unrelated males. The combined match probability (CMP) of the HG19+14Y System was calculated as 2.39 × 10-29. All the validation results showed that the HG19+14Y System would be a robust, reliable, highly polymorphic, and informative forensic kit.
authors.affiliation: School of Forensic Medicine, Southern Medical University, Guangzhou, 510515, China.
authors.forename: Weian
authors.initials: W
authors.lastname: Du
authors.affiliation: School of Forensic Medicine, Southern Medical University, Guangzhou, 510515, China.
authors.forename: Ling
authors.initials: L
authors.lastname: Chen
authors.affiliation: Guangzhou Forensic Science Institute, Guangdong Province Key Laboratory of Forensic Genetics, Guangzhou, 510030, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Liu
authors.affiliation: School of Forensic Medicine, Southern Medical University, Guangzhou, 510515, China.
authors.forename: Pingming
authors.initials: P
authors.lastname: Qiu
authors.affiliation: AGCU ScienTech Incorporation, Wuxi, 214174, China.
authors.forename: Fayuan
authors.initials: F
authors.lastname: Li
authors.affiliation: HOMY GeneTech Incorporation, Foshan, 518000, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Gao
authors.affiliation: HOMY GeneTech Incorporation, Foshan, 518000, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: HOMY GeneTech Incorporation, Foshan, 518000, China.
authors.forename: Bangchao
authors.initials: B
authors.lastname: Wang
authors.affiliation: School of Forensic Medicine, Southern Medical University, Guangzhou, 510515, China. liuchaogzf@163.com.|Guangzhou Forensic Science Institute, Guangdong Province Key Laboratory of Forensic Genetics, Guangzhou, 510030, China. liuchaogzf@163.com.
authors.forename: Chao
authors.initials: C
authors.lastname: Liu
mesh_terms: D053523:Amelogenin / Q000235:genetics*; D000818:Animals; D041322:Chromosomes, Human, Y; D016172:DNA Fingerprinting; D005006:Ethnicity / Q000235:genetics; D005260:Female; D005787:Gene Frequency; D005838:Genotype; D006801:Humans; D008297:Male; D018895:Microsatellite Repeats; D060885:Multiplex Polymerase Chain Reaction / Q000295:instrumentation*; D015203:Reproducibility of Results; D013045:Species Specificity
chemical_list: D053523:Amelogenin
keywords: DNA typing; Forensic science; Multiplex PCR; STR; Short tandem repeat; Validation