title: Expression of microRNA-328 Functions as a Biomarker for Recurrence of Early Gastric Cancer (EGC) After Endoscopic Submucosal Dissection (ESD) by Modulating CD44.
abstract: BACKGROUND This study investigated the molecular mechanism of the effect of CD44 on the recurrence of EGC after ESD, including the potential regulator and signaling pathways of CD44. MATERIAL AND METHODS We searched the miRNA online database (www.mirdb.org) with the "seed sequence" located within the 3'-UTR of the target gene, and performed luciferase assay to test the miRNA/mRNA relationship. We also determined the expression of CD44 in the EGC and control samples. In addition, statistical analysis was used to explore the role of miR-328 as a biomarker to predict the recurrence after ECD. RESULTS We validated CD44 to be the direct gene via luciferase reporter assay system. We also established the negative regulatory relationship between miR-328 and CD44 via studying the relative luciferase activity at different concentrations of miR-328 mimics. We also conducted real-time PCR and Western blot analysis to study the mRNA and protein expression level of CD44 among different groups (recurrence-positive and recurrence-negative) or cells treated with different concentrations of miR-328 mimics/inhibitors, indicating the negative regulatory relationship between miR-328 and CD44. We also investigated the relative viability of EGC cells when transfected with miR-328 mimics (50 nM and 100 nM) and miR-328 inhibitors (100 nM) to validate miR-328 to be negatively interfering with the viability of EGC cells. miR-328 was also recognized as a potential biomarker to predict recurrence after ESD in EGC patients via analysis of the recurrence-free rate among different groups of EGC patients. CONCLUSIONS The expression level of miR-328 can function as a predictive biomarker of recurrence after ECD in patients with EGC via targeting CD44.
authors.affiliation: Department of Gastroenterology, The Affiliated Hospital of Qingdao University, Qingdao, Shandong, China (mainland).
authors.forename: Hui-Guang
authors.initials: HG
authors.lastname: Xue
authors.affiliation: Department of Clinical Laboratory Medicine, The Affiliated Hospital of Qingdao University, Qingdao, Shandong, China (mainland).
authors.forename: Ai-Hua
authors.initials: AH
authors.lastname: Yang
authors.affiliation: Department of Gastroenterology, The Affiliated Hospital of Qingdao University, Qingdao, Shandong, China (mainland).
authors.forename: Xue-Guo
authors.initials: XG
authors.lastname: Sun
authors.affiliation: Department of Gastroenterology, The Affiliated Hospital of Qingdao University, Qingdao, Shandong, China (mainland).
authors.forename: Yan-Yan
authors.initials: YY
authors.lastname: Lu
authors.affiliation: Department of Gastroenterology, The Affiliated Hospital of Qingdao University, Qingdao, Shandong, China (mainland).
authors.forename: Zi-Bin
authors.initials: ZB
authors.lastname: Tian
mesh_terms: D020413:3' Untranslated Regions; D000328:Adult; D014408:Biomarkers, Tumor / Q000096:biosynthesis* / Q000097:blood / Q000235:genetics; D045744:Cell Line, Tumor; D000069916:Endoscopic Mucosal Resection / Q000379:methods; D016099:Endoscopy, Gastrointestinal / Q000379:methods; D005260:Female; D005773:Gastroscopy / Q000379:methods; D006801:Humans; D018960:Hyaluronan Receptors / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*; D008297:Male; D035683:MicroRNAs / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D008875:Middle Aged; D009364:Neoplasm Recurrence, Local / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D013274:Stomach Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology / Q000601:surgery; D016896:Treatment Outcome
chemical_list: D020413:3' Untranslated Regions; D014408:Biomarkers, Tumor; C497483:CD44 protein, human; D018960:Hyaluronan Receptors; C548170:MIRN328 microRNA, human; D035683:MicroRNAs