title: Zero-link polymerized hemoglobin (OxyVita®Hb) stabilizes the heme environment: potential for lowering vascular oxidative stress.
abstract: A study was conducted to compare the resistance to heme exposure between myoglobin, bovine hemoglobin, and OxyVita®Hb (zero-link-hemoglobin-polymer). The rate of release of heme-iron is related to the tissue oxidative stress that can elicit deleterious effects in clinical uses of blood substitutes. Experimental work has focused on the unfolding of hemoglobin molecules, resulting in heme loss, by using urea as a denaturant and analyzing the Soret spectral region. These unfolding studies provide evidence for both the structural integrity and redox stability of OxyVita®Hb and demonstrate that OxyVita®Hb does not readily unfold and its heme exposure/release is greatly reduced.
authors.affiliation: a OXYVITA Inc. , New Windsor , NY , USA.
authors.forename: Hanna
authors.initials: H
authors.lastname: Wollocko
authors.affiliation: b Department of Chemistry , State University of New York , New Paltz , NY , USA.
authors.forename: Sarah
authors.initials: S
authors.lastname: Anvery
authors.affiliation: a OXYVITA Inc. , New Windsor , NY , USA.
authors.forename: Jacek
authors.initials: J
authors.lastname: Wollocko
authors.affiliation: c Department of Biochemistry and Molecular Biology , University of Georgia , Athens , GA.
authors.forename: John M
authors.initials: JM
authors.lastname: Harrington
authors.affiliation: a OXYVITA Inc. , New Windsor , NY , USA.|b Department of Chemistry , State University of New York , New Paltz , NY , USA.
authors.forename: John P
authors.initials: JP
authors.lastname: Harrington
mesh_terms: D000818:Animals; D001802:Blood Substitutes / Q000737:chemistry / Q000494:pharmacology*; D001808:Blood Vessels / Q000187:drug effects* / Q000378:metabolism*; D002417:Cattle; D006418:Heme / Q000737:chemistry*; D006454:Hemoglobins / Q000737:chemistry / Q000494:pharmacology*; D018384:Oxidative Stress / Q000187:drug effects*; D055550:Protein Stability / Q000187:drug effects; D058767:Protein Unfolding / Q000187:drug effects
chemical_list: D001802:Blood Substitutes; D006454:Hemoglobins; C529085:OxyVita; D006418:Heme
keywords: Hemoglobin; OxyVita; blood substitute; oxidative stress; oxygen radicals; redox stability; zero-link-hemoglobin-polymer