title: High-throughput compound evaluation on 3D networks of neurons and glia in a microfluidic platform.
abstract: With great advances in the field of in vitro brain modelling, the challenge is now to implement these technologies for development and evaluation of new drug candidates. Here we demonstrate a method for culturing three-dimensional networks of spontaneously active neurons and supporting glial cells in a microfluidic platform. The high-throughput nature of the platform in combination with its compatibility with all standard laboratory equipment allows for parallel evaluation of compound effects.
authors.affiliation: MIMETAS BV, J.H. Oortweg 19, 2333 CH, Leiden, the Netherlands.|Department of Molecular Cell Biology, Leiden University Medical Centre, Leiden, the Netherlands.
authors.forename: Nienke R
authors.initials: NR
authors.lastname: Wevers
authors.affiliation: MIMETAS BV, J.H. Oortweg 19, 2333 CH, Leiden, the Netherlands.
authors.forename: Remko
authors.initials: R
authors.lastname: van Vught
authors.affiliation: MIMETAS BV, J.H. Oortweg 19, 2333 CH, Leiden, the Netherlands.
authors.forename: Karlijn J
authors.initials: KJ
authors.lastname: Wilschut
authors.affiliation: MIMETAS BV, J.H. Oortweg 19, 2333 CH, Leiden, the Netherlands.|Division of Analytical Biosciences, Leiden Academic Centre for Drug Research, Leiden University, the Netherlands.
authors.forename: Arnaud
authors.initials: A
authors.lastname: Nicolas
authors.affiliation: MIMETAS BV, J.H. Oortweg 19, 2333 CH, Leiden, the Netherlands.
authors.forename: Chiwan
authors.initials: C
authors.lastname: Chiang
authors.affiliation: MIMETAS BV, J.H. Oortweg 19, 2333 CH, Leiden, the Netherlands.
authors.forename: Henriette L
authors.initials: HL
authors.lastname: Lanz
authors.affiliation: MIMETAS BV, J.H. Oortweg 19, 2333 CH, Leiden, the Netherlands.
authors.forename: Sebastiaan J
authors.initials: SJ
authors.lastname: Trietsch
authors.affiliation: MIMETAS BV, J.H. Oortweg 19, 2333 CH, Leiden, the Netherlands.
authors.forename: Jos
authors.initials: J
authors.lastname: Joore
authors.affiliation: MIMETAS BV, J.H. Oortweg 19, 2333 CH, Leiden, the Netherlands.|Division of Analytical Biosciences, Leiden Academic Centre for Drug Research, Leiden University, the Netherlands.
authors.forename: Paul
authors.initials: P
authors.lastname: Vulto
mesh_terms: D000200:Action Potentials; D018929:Cell Culture Techniques / Q000295:instrumentation*; D048430:Cell Shape; D018920:Coculture Techniques; D003094:Collagen; D004338:Drug Combinations; D057166:High-Throughput Screening Assays / Q000379:methods*; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000166:cytology; D056656:Lab-On-A-Chip Devices; D007797:Laminin; D058953:Neural Stem Cells / Q000166:cytology*; D016501:Neurites / Q000648:ultrastructure; D055495:Neurogenesis; D009457:Neuroglia / Q000166:cytology / Q000187:drug effects*; D009474:Neurons / Q000166:cytology / Q000187:drug effects*; D009498:Neurotoxins / Q000494:pharmacology; D009940:Organoids / Q000187:drug effects*; D011509:Proteoglycans
chemical_list: D004338:Drug Combinations; D007797:Laminin; D009498:Neurotoxins; D011509:Proteoglycans; C059485:matrigel; D003094:Collagen