title: Generation and characterization of an induced pluripotent stem cell (iPSC) line from a patient with clozapine-resistant Schizophrenia.
abstract: Peripheral Blood Mononuclear Cells (PBMCs) were collected from a patient with clozapine-resistant (also known as "super-refractory") Schizophrenia. iPSCs were established with a non-integrating Sendai virus-based reprogramming system. A footprint-free hiPSC line was characterized to express the main endogenous pluripotency markers and to retain a normal karyotype. Cells showed pluripotency competency by giving rise to progeny of differentiated cells belonging to the three germ layers. This hiPSC line represents a valuable tool to obtain mature, pathology-relevant neuronal populations in vitro that are suitable to investigate the molecular background of the schizophrenic disorder and the resultant patients' response to treatments.
authors.affiliation: Laboratory of Stem Cell Biology, Centre for Integrative Biology - CIBIO, Università degli Studi di Trento, Trento, Italy. Electronic address: fmar@uni-bonn.de.
authors.forename: Fabio
authors.initials: F
authors.lastname: Marsoner
authors.affiliation: Laboratory of Stem Cell Biology, Centre for Integrative Biology - CIBIO, Università degli Studi di Trento, Trento, Italy; Department of Health Sciences, Università Degli Studi di Milano, Milan, Italy; Department of Mental Health, San Paolo Hospital, Milan, Italy.
authors.forename: Matteo
authors.initials: M
authors.lastname: Marcatili
authors.affiliation: Chromatin Dynamics Unit, San Raffaele University and Research Institute, Milan, Italy. Electronic address: tk2708@cumc.columbia.edu.
authors.forename: Thodoris
authors.initials: T
authors.lastname: Karnavas
authors.affiliation: Department of Health Sciences, Università Degli Studi di Milano, Milan, Italy.
authors.forename: Daniele
authors.initials: D
authors.lastname: Bottai
authors.affiliation: Department of Health Sciences, Università Degli Studi di Milano, Milan, Italy; Department of Mental Health, San Paolo Hospital, Milan, Italy.
authors.forename: Armando
authors.initials: A
authors.lastname: D'Agostino
authors.affiliation: Department of Health Sciences, Università Degli Studi di Milano, Milan, Italy; Department of Mental Health, San Paolo Hospital, Milan, Italy. Electronic address: Silvio.Scarone@unimi.it.
authors.forename: Silvio
authors.initials: S
authors.lastname: Scarone
authors.affiliation: Laboratory of Stem Cell Biology, Centre for Integrative Biology - CIBIO, Università degli Studi di Trento, Trento, Italy. Electronic address: Luciano.Conti@unitn.it.
authors.forename: Luciano
authors.initials: L
authors.lastname: Conti
mesh_terms: D014150:Antipsychotic Agents / Q000627:therapeutic use*; D002454:Cell Differentiation; D002460:Cell Line; D065150:Cellular Reprogramming; D003024:Clozapine / Q000627:therapeutic use*; D058732:Embryoid Bodies / Q000166:cytology / Q000378:metabolism; D005822:Genetic Vectors / Q000235:genetics / Q000378:metabolism; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000166:cytology* / Q000378:metabolism; D059785:Karyotype; D007963:Leukocytes, Mononuclear / Q000166:cytology; D008297:Male; D008856:Microscopy, Fluorescence; D012559:Schizophrenia / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D029082:Sendai virus / Q000235:genetics; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism
chemical_list: D014150:Antipsychotic Agents; D014157:Transcription Factors; D003024:Clozapine