title: Rapid identification of Candida spp. frequently involved in invasive mycoses by using flow-through hybridization and Gene Chip (FHGC) technology.
abstract: The incidence of invasive fungal infections in immunocompromised patients has increased in recent decades. Rapid and accurate identification of these pathogenic fungi is crucial for initiating a timely, safe, and effective antifungal therapy. Here we developed a microarray based on flow-through hybridization gene chip technology. The microarray was tested for its specificity using a panel of reference and blinded clinical isolates. The results proved that this microarray was highly discriminative, leading to the unequivocal identification of each species, including Candida famata and the highly related species Candida parapsilosis, Candida orthopsilosis, and Candida metapsilosis. This new system represents a reliable method that is of potential use in clinical laboratories for the simultaneous detection and identification of the most common pathogenic fungi.
authors.affiliation: School of Rehabilitation Medicine, Capital Medical University, China. Electronic address: lilycrrc@sina.com.
authors.forename: Chen
authors.initials: C
authors.lastname: Li
authors.affiliation: Departments of Clinical Laboratory, Beijing Youan Hospital, Capital Medical University, Beijing 100069, China. Electronic address: Dingxr586@126.com.
authors.forename: Xiurong
authors.initials: X
authors.lastname: Ding
authors.affiliation: School of Rehabilitation Medicine, Capital Medical University, China. Electronic address: lzzlab@126.com.
authors.forename: Zhizhong
authors.initials: Z
authors.lastname: Liu
authors.affiliation: Chaozhou Hybribio Limited Corporation, Chaozhou 521011, China. Electronic address: jjzhu@hybribio.cn.
authors.forename: Juanjuan
authors.initials: J
authors.lastname: Zhu
mesh_terms: D000935:Antifungal Agents / Q000494:pharmacology; D002175:Candida / Q000145:classification / Q000302:isolation & purification*; D004271:DNA, Fungal / Q000302:isolation & purification*; D025141:Drug Resistance, Fungal; D005240:Feasibility Studies; D006801:Humans; D000072742:Invasive Fungal Infections / Q000175:diagnosis* / Q000382:microbiology; D008826:Microbial Sensitivity Tests; D016533:Mycological Typing Techniques; D009693:Nucleic Acid Hybridization; D020411:Oligonucleotide Array Sequence Analysis; D017422:Sequence Analysis, DNA; D013048:Specimen Handling
chemical_list: D000935:Antifungal Agents; D004271:DNA, Fungal
keywords: Candida spp.; Flow-through hybridization and gene chip; Identification; Microarray; Rapid