title: Use of polyvinylpyrrolidone-iodine solution for sterilisation and preservation improves mechanical properties and osteogenesis of allografts.
abstract: Allografts eliminate the disadvantages associated with autografts and synthetic scaffolds but are associated with a disease-transmission risk. Therefore, allograft sterilisation is crucial. We aimed to determine whether polyvinylpyrrolidone-iodine (PVP-I) can be used for sterilisation and as a new wet-preservation method. PVP-I-sterilised and preserved allografts demonstrated improved mechanical property, osteogenesis, and excellent microbial inhibition. A thigh muscle pouch model of nude mice showed that PVP-I-preserved allografts demonstrated better ectopic formation than Co60-sterilised allografts (control) in vivo (P < 0.05). Furthermore, the PVP-I-preserved group showed no difference between 24 h and 12 weeks of allograft preservation (P > 0.05). PVP-I-preserved allografts showed more hydrophilic surfaces and PVP-I-sterilised tendons showed higher mechanical strength than Co60-sterilised tendons (P < 0.05). The level of residual PVP-I was higher without washing and with prolonged preservation (P < 0.05). In vitro cellular tests showed that appropriate PVP-I concentration was nontoxic to preosteoblast cells, and cellular differentiation measured by alkaline phosphatase activity and osteogenic gene markers was enhanced (P < 0.05). Therefore, the improved biological performance of implanted allografts may be attributable to better surface properties and residual PVP-I, and PVP-I immersion can be a simple, easy method for allograft sterilisation and preservation.
authors.affiliation: Beijing Engineering Research Center of Orthopaedic Implants, First Affiliated Hospital of CPLA General Hospital, Beijing 100048, P. R. China.
authors.forename: Yantao
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: Beijing Engineering Research Center of Orthopaedic Implants, First Affiliated Hospital of CPLA General Hospital, Beijing 100048, P. R. China.
authors.forename: Xiantong
authors.initials: X
authors.lastname: Hu
authors.affiliation: Beijing Engineering Research Center of Orthopaedic Implants, First Affiliated Hospital of CPLA General Hospital, Beijing 100048, P. R. China.
authors.forename: Zhonghai
authors.initials: Z
authors.lastname: Li
authors.affiliation: Beijing Engineering Research Center of Orthopaedic Implants, First Affiliated Hospital of CPLA General Hospital, Beijing 100048, P. R. China.
authors.forename: Fuli
authors.initials: F
authors.lastname: Wang
authors.affiliation: Jiangsu Key Laboratory of Oral Diseases, Nanjing Medical University, Nanjing 210029, P. R. China.|State Key Laboratory of Bioelectronics, Jiangsu Key Laboratory of Biomaterials and Devices, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, P. R. China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Xia
authors.affiliation: Beijing Engineering Research Center of Orthopaedic Implants, First Affiliated Hospital of CPLA General Hospital, Beijing 100048, P. R. China.
authors.forename: Shuxun
authors.initials: S
authors.lastname: Hou
authors.affiliation: Beijing Engineering Research Center of Orthopaedic Implants, First Affiliated Hospital of CPLA General Hospital, Beijing 100048, P. R. China.
authors.forename: Hongbin
authors.initials: H
authors.lastname: Zhong
authors.affiliation: Jiangsu Key Laboratory of Oral Diseases, Nanjing Medical University, Nanjing 210029, P. R. China.|Suzhou Institute &Collaborative Innovation Center of Suzhou Nano Science and Technology, Southeast University, Suzhou 215000, P. R. China.
authors.forename: Feimin
authors.initials: F
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Bioelectronics, Jiangsu Key Laboratory of Biomaterials and Devices, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, P. R. China.|Suzhou Institute &Collaborative Innovation Center of Suzhou Nano Science and Technology, Southeast University, Suzhou 215000, P. R. China.
authors.forename: Ning
authors.initials: N
authors.lastname: Gu
mesh_terms: D000818:Animals; D015415:Biomarkers; D016025:Bone Transplantation; D001842:Bone and Bones / Q000000981:diagnostic imaging / Q000187:drug effects* / Q000502:physiology* / Q000648:ultrastructure; D007150:Immunohistochemistry; D008297:Male; D055595:Mechanical Phenomena / Q000187:drug effects; D051379:Mice; D019188:Organ Preservation Solutions / Q000494:pharmacology*; D010012:Osteogenesis / Q000187:drug effects*; D011206:Povidone-Iodine / Q000494:pharmacology*; D011309:Preservation, Biological; D013242:Sterilization; D014184:Transplantation, Homologous
chemical_list: D015415:Biomarkers; D019188:Organ Preservation Solutions; D011206:Povidone-Iodine