title: Integrating insulin into single-step culture medium regulates human embryo development in vitro.
abstract: To evaluate the effect of supplementing single-step embryo culture medium with insulin on human embryo development.
  
  
  
  Comparative study.
  
  
  
  Two private centers.
  
  
  
  The study involved a sibling oocyte split of 5,142 retrieved oocytes from 360 patients.
  
  
  
  Sibling oocytes split after intracytoplasmic sperm injection for culture from day 0 through day 5 or 6 in insulin-supplemented or control medium. Women were split to receive their embryos from insulin-supplemented or control medium.
  
  
  
  Clinical pregnancy rate.
  
  
  
  There were significantly higher rates of clinical, ongoing, and twin pregnancies in the insulin-supplemented arm than in the control arm. On day 3, embryo quality and compaction were higher in insulin-supplemented medium. On day 5, insulin supplementation showed higher rates of blastocyst formation, quality, and cryopreservation.
  
  
  
  Insulin supplementation of single-step embryo culture medium from day 0 through day 5 or 6 improved clinical pregnancy rate and human embryo development. However, these findings need further confirmation through a multicenter randomized controlled trial that may include other patient populations and different culture media.
authors.affiliation: IbnSina IVF Center, IbnSina Hospital, Sohag, Egypt. Electronic address: drfawzy001@me.com.
authors.forename: Mohamed
authors.initials: M
authors.lastname: Fawzy
authors.affiliation: Department of Obstetrics and Gynecology, Faculty of Medicine, Sohag University, Sohag, Egypt.
authors.forename: Mohamed
authors.initials: M
authors.lastname: Sabry
authors.affiliation: Department of Obstetrics and Gynecology, Faculty of Medicine, Sohag University, Sohag, Egypt.
authors.forename: Mohamed
authors.initials: M
authors.lastname: Nour
authors.affiliation: Department of Obstetrics and Gynecology, Faculty of Medicine, Sohag University, Sohag, Egypt.
authors.forename: Mohamed Y
authors.initials: MY
authors.lastname: Abdelrahman
authors.affiliation: Department of Public Health and Community Medicine, Sohag University, Sohag, Egypt.
authors.forename: Eman
authors.initials: E
authors.lastname: Roshdy
authors.affiliation: Dar Almaraa Fertility and Gynecology Center, Cairo, Egypt.
authors.forename: Yasmin
authors.initials: Y
authors.lastname: Magdi
authors.affiliation: Department of Obstetrics and Gynecology, Faculty of Medicine, Sohag University, Sohag, Egypt.
authors.forename: Hazem
authors.initials: H
authors.lastname: Abdelghafar
mesh_terms: D000293:Adolescent; D000328:Adult; D001755:Blastocyst / Q000187:drug effects*; D015925:Cryopreservation; D003470:Culture Media / Q000009:adverse effects / Q000737:chemistry*; D004534:Egypt; D046149:Embryo Culture Techniques; D004624:Embryo Transfer; D047108:Embryonic Development / Q000187:drug effects; D005260:Female; D005298:Fertility; D005300:Fertility Agents, Female / Q000009:adverse effects / Q000627:therapeutic use*; D006801:Humans; D007246:Infertility / Q000175:diagnosis / Q000503:physiopathology / Q000628:therapy*; D007328:Insulin / Q000009:adverse effects / Q000627:therapeutic use*; D008297:Male; D054315:Oocyte Retrieval; D011247:Pregnancy; D018873:Pregnancy Rate; D059285:Pregnancy, Twin; D011446:Prospective Studies; D020554:Sperm Injections, Intracytoplasmic / Q000009:adverse effects; D013997:Time Factors; D016896:Treatment Outcome; D055815:Young Adult
chemical_list: D003470:Culture Media; D005300:Fertility Agents, Female; D007328:Insulin
keywords: Embryo culture media; blastocyst; insulin embryonic effect