title: Antioxidant properties of coenzyme Q10-pretreated mouse pre-antral follicles derived from vitrified ovaries.
abstract: OBJECTIVE
  This study evaluated the antioxidant status of pre-antral follicles derived from vitrified ovaries pretreated with coenzyme Q10 (CoQ10).
  
  
  METHODS
  Mouse pre-antral follicles derived from fresh and vitrified warmed ovarian tissue were cultured with or without CoQ10 (50 μmol/L). Follicular growth, total antioxidant capacity (TAC), malondialdehyde (MDA) level, and superoxide dismutase (SOD), glutathione peroxidase (GPX), and catalase (CAT) activity during cultivation were assessed.
  
  
  RESULTS
  The growth rate of the fresh pre-antral follicles was higher compared with the vitrified groups, especially in the CoQ10-treated than non-treated groups. MDA increased while TAC decreased at 96 h of the cultivation period. TAC was higher while MDA was lower in the fresh pre-antral follicles than in the vitrified groups. These rates were higher in the CoQ10-treated than non-treated groups. The vitrified and fresh CoQ10-pretreated groups had significantly higher SOD, GPX, and CAT activity compared with the CoQ10 non-treated groups.
  
  
  CONCLUSIONS
  CoQ10-supplemented maturation medium can increase antioxidant enzyme activity and decrease lipid peroxidation in cultured pre-antral follicles derived from fresh and vitrified mouse ovaries.
authors.affiliation: School of Biology, Damghan University, Damghan, Iran.
authors.forename: Elmira
authors.initials: E
authors.lastname: Hosseinzadeh
authors.affiliation: School of Biology, Damghan University, Damghan, Iran.|Institute of Biological Sciences, Damghan University, Damghan, Iran.
authors.forename: Saeed
authors.initials: S
authors.lastname: Zavareh
authors.affiliation: School of Biology, Damghan University, Damghan, Iran.|Institute of Biological Sciences, Damghan University, Damghan, Iran.
authors.forename: Taghi
authors.initials: T
authors.lastname: Lashkarbolouki
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D002374:Catalase / Q000378:metabolism; D005260:Female; D005979:Glutathione Peroxidase / Q000378:metabolism; D008315:Malondialdehyde / Q000378:metabolism; D051379:Mice; D006080:Ovarian Follicle / Q000187:drug effects* / Q000201:enzymology*; D013482:Superoxide Dismutase / Q000378:metabolism; D014451:Ubiquinone / Q000031:analogs & derivatives* / Q000494:pharmacology; D058989:Vitrification
chemical_list: D000975:Antioxidants; D014451:Ubiquinone; D008315:Malondialdehyde; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; C024989:coenzyme Q10
keywords: antioxidant enzyme; coenzyme Q10; ovary; vitrification