title: [Effects of (Pyr1)apelin-13 on the proliferation and activation of Jarkat T lymphocyte].
abstract: Objective To investigate the effect of (Pyr1)apelin-13 on the proliferation and activation of Jurkat T lymphocytes. Methods Jurkat T cells were treated by 0, 10, 50 nmol/L (Pyr1)apelin-13 for 24 hours and cell proliferation ability was detected by CCK-8 assay. The effect of (Pyr1)apelin-13 on the activation of Jurkat T lymphocytes with anti-CD3 and anti-CD28 antibodies was observed and the molecular mechanism was investigated. Quantitative real-time PCR was applied to detect the expressions of CD69, CD25, cytotoxic T lymphocyte antigen-4 (CTLA-4) and programmed death-1 (PD-1) mRNAs. Results The proliferation of Jurkat T cells was not affected by (Pyr1) apelin-13. However, (Pyr1)apelin-13 increased the expressions of CD69 and CD25 and then activated Jurkat T lymphocytes. Furthermore, the expressions of CTLA-4 and PD-1 had no difference between activation group and (Pyr1)apelin-13 group. Conclusion (Pyr1)apelin-13 promotes Jurkat T lymphocyte activation by increasing the expressions of CD69 and CD25.
authors.affiliation: Department of Pharmacology, Xi'an Medical University, Xi'an 710021, China.
authors.forename: Ming
authors.initials: M
authors.lastname: Wei
authors.affiliation: Department of Gynecology, Shaanxi Provincial People's Hospital, Xi'an 710068, China. *Corresponding author, E-mail: ganlu-789@163.com.
authors.forename: Lu
authors.initials: L
authors.lastname: Gan
authors.affiliation: Department of Pharmacology, Xi'an Medical University, Xi'an 710021, China.
authors.forename: Jin
authors.initials: J
authors.lastname: Hou
authors.affiliation: Department of Gynecology, Shaanxi Provincial People's Hospital, Xi'an 710068, China.
authors.forename: Lihong
authors.initials: L
authors.lastname: Chen
authors.affiliation: Department of Biochemistry and Molecular Biology, Xi'an Medical University, Xi'an 710021, China.
authors.forename: Yantong
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Department of Biochemistry and Molecular Biology, Xi'an Medical University, Xi'an 710021, China.
authors.forename: Ligang
authors.initials: L
authors.lastname: Ren
mesh_terms: D017209:Apoptosis / Q000235:genetics; D017252:CD3 Complex / Q000276:immunology; D060908:CTLA-4 Antigen / Q000235:genetics; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins / Q000494:pharmacology*; D019169:Jurkat Cells / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D008213:Lymphocyte Activation / Q000187:drug effects*; D012333:RNA, Messenger / Q000235:genetics
chemical_list: D017252:CD3 Complex; D060908:CTLA-4 Antigen; D036341:Intercellular Signaling Peptides and Proteins; D012333:RNA, Messenger; C495199:apelin-13 peptide