title: Dicranostiga leptopodu (Maxim.) Fedde extracts attenuated CCl4-induced acute liver damage in mice through increasing anti-oxidative enzyme activity to improve mitochondrial function.
abstract: Dicranostiga Leptodu (Maxim.) fedde (DLF), a poppy plant, has been reported have many benefits and medicinal properties, including free radicals scavenging and detoxifying. However, the protective effect of DLF extracts against carbon tetrachloride (CCl4)-induced damage in mice liver has not been elucidated. Here, we demonstrated that DLF extracts attenuated CCl4-induced liver damage in mice through increasing anti-oxidative enzyme activity to improve mitochondrial function. In this study, the mice liver damage evoked by CCl4was marked by morphology changes, significant rise in lipid peroxidation, as well as alterations of mitochondrial respiratory function. Interestingly, pretreatment with DLF extracts attenuated CCl4-induced morphological damage and increasing of lipid peroxidation in mice liver. Additionally, DLF extracts improved mitochondrial function by preventing the disruption of respiratory chain and suppression of mitochondrial Na+K+-ATPase and Ca2+-ATPase activity. Furthermore, administration with DLF extracts elevated superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx) levels and maintained the balance of redox status. This results showed that toxic protection effect of DLF extracts on mice liver is mediated by improving mitochondrial respiratory function and keeping the balance of redox status, which suggesting that DLF extracts could be used as potential toxic protection agent for the liver against hepatotoxic agent.
authors.affiliation: School of Chemical & Biological Engineering, Lanzhou Jiaotong University, Lanzhou 730070, PR China.
authors.forename: Deping
authors.initials: D
authors.lastname: Tang
authors.affiliation: Institute of Gansu Medical Science Research, Lanzhou 730050, PR China.
authors.forename: Fang
authors.initials: F
authors.lastname: Wang
authors.affiliation: Institute of Gansu Medical Science Research, Lanzhou 730050, PR China; School of life science, Lanzhou University, Lanzhou 730000, PR China.
authors.forename: Jinzhou
authors.initials: J
authors.lastname: Tang
authors.affiliation: Institute of Gansu Medical Science Research, Lanzhou 730050, PR China; School of life science, Lanzhou University, Lanzhou 730000, PR China. Electronic address: maoaih@aliyun.com.
authors.forename: Aihong
authors.initials: A
authors.lastname: Mao
authors.affiliation: Institute of Gansu Medical Science Research, Lanzhou 730050, PR China.
authors.forename: Shiqi
authors.initials: S
authors.lastname: Liao
authors.affiliation: School of life science, Lanzhou University, Lanzhou 730000, PR China.
authors.forename: Qin
authors.initials: Q
authors.lastname: Wang
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D000252:Calcium-Transporting ATPases / Q000235:genetics / Q000378:metabolism; D002252:Carbon Tetrachloride Poisoning / Q000188:drug therapy*; D056486:Chemical and Drug Induced Liver Injury / Q000473:pathology / Q000517:prevention & control*; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D007928:Lethal Dose 50; D051379:Mice; D008928:Mitochondria / Q000187:drug effects* / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects; D028101:Papaveraceae / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D000254:Sodium-Potassium-Exchanging ATPase / Q000235:genetics / Q000378:metabolism
chemical_list: D000975:Antioxidants; D010936:Plant Extracts; D000252:Calcium-Transporting ATPases; D000254:Sodium-Potassium-Exchanging ATPase
keywords: CCl(4); DLF extracts; Liver damage; Mitochondrial function; Redox status