title: Cytogenetic Harvesting of Cancer Cells and Cell Lines.
abstract: We describe an evidence-based approach toward optimizing chromosome preparation from cancer cells and cell lines. The procedures described here emphasize the utility of both cell culture-to maximize the yields of the dividing cells needed to harvest mitotic metaphase chromosome preparations and how an empirical evaluation of hypotonic treatments enables optimal conditions to be efficiently determined.
authors.affiliation: Department of Human and Animal Cell Lines, German Collection of Microorganisms and Cell Cultures, Leibniz Institute-DSMZ, Inhoffenstr. 7b, 38124, Braunschweig, Germany. rml@dsmz.de.
authors.forename: Roderick A F
authors.initials: RA
authors.lastname: MacLeod
authors.affiliation: Department of Human and Animal Cell Lines, German Collection of Microorganisms and Cell Cultures, Leibniz Institute-DSMZ, Inhoffenstr. 7b, 38124, Braunschweig, Germany.
authors.forename: Maren E
authors.initials: ME
authors.lastname: Kaufmann
authors.affiliation: Department of Human and Animal Cell Lines, German Collection of Microorganisms and Cell Cultures, Leibniz Institute-DSMZ, Inhoffenstr. 7b, 38124, Braunschweig, Germany.
authors.forename: Hans G
authors.initials: HG
authors.lastname: Drexler
mesh_terms: D045744:Cell Line, Tumor; D002869:Chromosome Aberrations; D002871:Chromosome Banding / Q000379:methods; D020732:Cytogenetic Analysis / Q000379:methods; D006801:Humans; D007621:Karyotyping / Q000379:methods; D009369:Neoplasms / Q000175:diagnosis / Q000235:genetics*; D014407:Tumor Cells, Cultured
keywords: Cancer chromosomes; Cell culture; Fixation; Hypotonic treatment; Slide making