title: Real-time imaging of the growth-inhibitory effect of JS399-19 on Fusarium.
abstract: Real-time imaging was used to study the effects of a novel Fusarium-specific cyanoacrylate fungicide (JS399-19) on growth and morphology of four Fusarium sp. This fungicide targets the motor domain of type I myosin. Fusarium graminearum PH-1, Fusarium solani f. sp. pisi 77-13-4, Fusarium avenaceum IBT8464, and Fusarium avenaceum 05001, which has a K216Q amino-acid substitution at the resistance-implicated site in its myosin type I motor domain, were analyzed. Real-time imaging shows that JS399-19 inhibits fungal growth but not to the extent previously reported. The fungicide causes the hypha to become entangled and unable to extend vertically. This implies that type I myosin in Fusarium is essential for hyphal and mycelia propagation. The K216Q substitution correlates with reduced susceptibility in F. avenaceum.
authors.affiliation: Department of Chemistry and Bioscience, Aalborg University, DK-9220 Aalborg, Denmark. Electronic address: rwo@bio.aau.dk.
authors.forename: Rasmus D
authors.initials: RD
authors.lastname: Wollenberg
authors.affiliation: Department of Chemistry and Bioscience, Aalborg University, DK-9220 Aalborg, Denmark.
authors.forename: Søren S
authors.initials: SS
authors.lastname: Donau
authors.affiliation: Department of Chemistry and Bioscience, Aalborg University, DK-9220 Aalborg, Denmark.
authors.forename: Thorbjørn T
authors.initials: TT
authors.lastname: Nielsen
authors.affiliation: Department of Chemistry and Bioscience, Aalborg University, DK-9220 Aalborg, Denmark.
authors.forename: Jens L
authors.initials: JL
authors.lastname: Sørensen
authors.affiliation: Department of Chemistry and Bioscience, Aalborg University, DK-9220 Aalborg, Denmark.
authors.forename: Henriette
authors.initials: H
authors.lastname: Giese
authors.affiliation: Department of Chemistry and Bioscience, Aalborg University, DK-9220 Aalborg, Denmark.
authors.forename: Reinhard
authors.initials: R
authors.lastname: Wimmer
authors.affiliation: Department of Chemistry and Bioscience, Aalborg University, DK-9220 Aalborg, Denmark.
authors.forename: Teis E
authors.initials: TE
authors.lastname: Søndergaard
mesh_terms: D000595:Amino Acid Sequence; D000596:Amino Acids / Q000494:pharmacology*; D025141:Drug Resistance, Fungal / Q000235:genetics; D005656:Fungal Proteins / Q000737:chemistry; D005659:Fungicides, Industrial / Q000494:pharmacology*; D005670:Fusarium / Q000166:cytology / Q000187:drug effects* / Q000235:genetics / Q000254:growth & development; D025301:Hyphae / Q000187:drug effects / Q000254:growth & development; D008853:Microscopy; D024461:Myosin Type I / Q000737:chemistry; D010666:Phenylpropionates / Q000494:pharmacology*
chemical_list: C540078:2-cyano-3-amino-3-phenylacrylic acid; D000596:Amino Acids; D005656:Fungal Proteins; D005659:Fungicides, Industrial; D010666:Phenylpropionates; D024461:Myosin Type I
keywords: Antimicrobial susceptibility testing; Cyanoacrylates; Fungicide resistance; Fungicides; Fusarium; Real-time microscopy