title: Characterization ofBrassica napusL. genotypes utilizing sequence-related amplified polymorphism and genotyping by sequencing in association with cluster analysis.
abstract: Identifying parental combinations that exhibit high heterosis is a constant target for commercialBrassica napusL. hybrid development programs. Finding high heterotic parental combinations can require hundreds of test crosses and years of yield evaluation. Heterotic pool development could be used to divide breeding material into specific breeding pools and focus the number of parental combinations created. Here, we report the genotypic characterization of 79B. napusgenotypes by calculating genetic distance based on sequence-related amplified polymorphism (SRAP) and genotyping by sequencing (GBS) in association with a neighbour-joining clustering algorithm. Despite the different genotypic analyses, neighbour-joining cluster analysis based on genetic distance of SRAP and GBS produced similar clusters. Homology between SRAP and GBS clusters was approximately 77 % when manually comparing clusters and 68 % when comparing clusters using Compare2Trees. This research demonstrates that SRAP can have similar efficacy when compared to next-generation sequencing technology for heterotic pool classification. This information may provide an important breeding scaffold for the development of hybrid cultivars based upon genetic distance and cluster analysis.
authors.affiliation: Faculty of Agricultural and Food Sciences, Department of Plant Science, University of Manitoba, 222 Agriculture Building, Winnipeg, MB R3T 2N2 Canada.
authors.forename: Corey J
authors.initials: CJ
authors.lastname: Lees
authors.affiliation: Faculty of Agricultural and Food Sciences, Department of Plant Science, University of Manitoba, 222 Agriculture Building, Winnipeg, MB R3T 2N2 Canada.
authors.forename: Genyi
authors.initials: G
authors.lastname: Li
authors.affiliation: Faculty of Agricultural and Food Sciences, Department of Plant Science, University of Manitoba, 222 Agriculture Building, Winnipeg, MB R3T 2N2 Canada.
authors.forename: Robert W
authors.initials: RW
authors.lastname: Duncan
keywords: Brassica napus; Cluster analysis; GBS; Genetic distance; Heterosis; SRAP