title: Rapid assessment of tetanus vaccine-induced immunity in Bangladesh and the Gambia.
abstract: We have developed recombinant fragment C based rapid point of care dipstick devices to assess tetanus immunization status using plasma or whole blood. The devices demonstrated specificity of 0.90 and sensitivity of 0.90 (whole blood)/0.94 (plasma) at field sites in Bangladesh and The Gambia when compared to a commercial ELISA with the immune cut-off titer set as ≥0.1IU/mL.
authors.affiliation: Department of Medicine/Division of Infectious Disease and International Health, University of Virginia, Charlottesville, VA, USA.
authors.forename: Girija
authors.initials: G
authors.lastname: Ramakrishnan
authors.affiliation: TechLab Inc., Blacksburg, VA, USA.
authors.forename: Marcia
authors.initials: M
authors.lastname: Wright
authors.affiliation: International Centre for Diarrhoeal Disease Research, Bangladesh, Dhaka, Bangladesh (icddr,b).
authors.forename: Masud
authors.initials: M
authors.lastname: Alam
authors.affiliation: Nutrition Theme, MRC Unit The Gambia (MRCG), Banjul, The Gambia.
authors.forename: Caitlin
authors.initials: C
authors.lastname: Naylor
authors.affiliation: International Centre for Diarrhoeal Disease Research, Bangladesh, Dhaka, Bangladesh (icddr,b).
authors.forename: Mamun
authors.initials: M
authors.lastname: Kabir
authors.affiliation: International Centre for Diarrhoeal Disease Research, Bangladesh, Dhaka, Bangladesh (icddr,b).
authors.forename: Ayesha
authors.initials: A
authors.lastname: Zerin
authors.affiliation: International Centre for Diarrhoeal Disease Research, Bangladesh, Dhaka, Bangladesh (icddr,b).
authors.forename: Tahsin
authors.initials: T
authors.lastname: Ferdous
authors.affiliation: TechLab Inc., Blacksburg, VA, USA.
authors.forename: Karl
authors.initials: K
authors.lastname: Pedersen
authors.affiliation: Nutrition Theme, MRC Unit The Gambia (MRCG), Banjul, The Gambia; MRC International Nutrition Group, London, School of Hygiene & Tropical Medicine, London, UK.
authors.forename: Branwen J
authors.initials: BJ
authors.lastname: Hennig
authors.affiliation: Division of Pediatric Infectious Diseases, Children's Hospital of Richmond at Virginia Commonwealth University, Richmond.
authors.forename: Jeffrey R
authors.initials: JR
authors.lastname: Donowitz
authors.affiliation: Nutrition Theme, MRC Unit The Gambia (MRCG), Banjul, The Gambia; MRC International Nutrition Group, London, School of Hygiene & Tropical Medicine, London, UK.
authors.forename: Rita
authors.initials: R
authors.lastname: Wegmuller
authors.affiliation: International Centre for Diarrhoeal Disease Research, Bangladesh, Dhaka, Bangladesh (icddr,b).
authors.forename: Rashidul
authors.initials: R
authors.lastname: Haque
authors.affiliation: Department of Medicine/Division of Infectious Disease and International Health, University of Virginia, Charlottesville, VA, USA.
authors.forename: William A
authors.initials: WA
authors.lastname: Petri
authors.affiliation: TechLab Inc., Blacksburg, VA, USA.
authors.forename: Joel
authors.initials: J
authors.lastname: Herbein
authors.affiliation: Department of Medicine/Division of Infectious Disease and International Health, University of Virginia, Charlottesville, VA, USA. Electronic address: cg2p@eservices.virginia.edu.
authors.forename: Carol A
authors.initials: CA
authors.lastname: Gilchrist
mesh_terms: D000328:Adult; D000907:Antibodies, Bacterial / Q000097:blood*; D001459:Bangladesh; D002648:Child; D005714:Gambia; D006801:Humans; D008297:Male; D010446:Peptide Fragments / Q000235:genetics / Q000276:immunology*; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology*; D012680:Sensitivity and Specificity; D013742:Tetanus / Q000276:immunology*; D013744:Tetanus Toxin / Q000235:genetics / Q000276:immunology*; D013745:Tetanus Toxoid / Q000276:immunology*; D014611:Vaccination
chemical_list: D000907:Antibodies, Bacterial; D010446:Peptide Fragments; D011994:Recombinant Proteins; D013744:Tetanus Toxin; D013745:Tetanus Toxoid; C040635:tetanus toxin fragment C
keywords: Dipstick; Fragment C; Lateral flow; Tetanus; Vaccine