title: Identification of Small Molecule Modulators of MicroRNA by Library Screening.
abstract: MicroRNAs (miRNAs) function as oncogenes or tumor suppressors and are dysregulated in cancer. miRNAs therefore represent promising therapeutic targets for cancer. Small molecules that could modulate the expression of miRNAs would thus have potential as anticancer agents. Library screening of small molecules targeting miRNAs is a useful technology platform for anticancer drug development. Here, we describe a hepatocellular carcinoma (HCC) cell-based luciferase reporter system which could be used to screen for small molecule modulators of tumor suppressor microRNA-34a.
authors.affiliation: School of Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong Kong, Shatin, Hong Kong, China.
authors.forename: Zhangang
authors.initials: Z
authors.lastname: Xiao
authors.affiliation: School of Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong Kong, Shatin, Hong Kong, China. frankchen@cuhk.edu.hk.|State Key Laboratory of Digestive Disease, Institute of Digestive Disease, The Chinese University of Hong Kong, Shatin, Hong Kong, China. frankchen@cuhk.edu.hk.|Shenzhen Research Institute, The Chinese University of Hong Kong, Shenzhen, 518087, China. frankchen@cuhk.edu.hk.
authors.forename: Yangchao
authors.initials: Y
authors.lastname: Chen
mesh_terms: D000970:Antineoplastic Agents / Q000737:chemistry / Q000627:therapeutic use; D045744:Cell Line, Tumor; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D017930:Genes, Reporter; D006801:Humans; D008156:Luciferases / Q000235:genetics; D035683:MicroRNAs / Q000037:antagonists & inhibitors* / Q000737:chemistry / Q000235:genetics; D008967:Molecular Biology / Q000379:methods*; D009369:Neoplasms / Q000235:genetics* / Q000628:therapy; D054852:Small Molecule Libraries / Q000737:chemistry* / Q000627:therapeutic use
chemical_list: D000970:Antineoplastic Agents; D035683:MicroRNAs; D054852:Small Molecule Libraries; D008156:Luciferases
keywords: Cancer; Library screening; Small molecule; microRNA