title: Profiling and Identification of Novel Immunogenic Proteins of Staphylococcus hyicus ZC-4 by Immunoproteomic Assay.
abstract: Staphylococcus hyicus has caused great losses in the swine industry by inducing piglet exudative epidermitis (EE), sow mastitis, metritis, and other diseases and is a threat to human health. The pathogenesis of EE, sow mastitis, and metritis involves the interaction between the host and virulent protein factors of S. hyicus, however, the proteins that interact with the host, especially the host immune system, are unclear. In the present study, immunoproteomics was used to screen the immunogenic proteins of S. hyicus strain ZC-4. The cellular and secreted proteins of S. hyicus strain ZC-4 were obtained, separated by 2D gel electrophoresis, and further analyzed by western blot with S. hyicus strain ZC-4-infected swine serum. Finally, 28 specific immunogenic proteins including 15 cellular proteins and 13 secreted proteins, 26 of which were novel immunogenic proteins from S. hyicus, were identified by matrix-assisted laser desorption ionization time-of-flight mass spectrometry. To further verify their immunogenicity, two representative proteins (acetate kinase [cellular] and enolase [secreted]) were chosen for expression, and the resultant recombinant proteins could react with S. hyicus ZC-4-infected swine serum. In mice, both acetate kinase and enolase activated the immune response by increasing G-CSF and MCP-5 expression, and acetate kinase further activated the immune response by increasing IL-12 expression. Enolase can confer better protection against S.hycius than acetate kinase in mice. For the first time to our knowledge, our results provide detailed descriptions of the cellular and secreted proteins of S. hyicus strain ZC-4. These immunogenic proteins may contribute to investigation and elucidation of the pathogenesis of S. hyicus and provide new candidates for subunit vaccines in the future.
authors.affiliation: College of Animal Science & National Engineering Center for Swine Breeding Industry, South China Agriculture University, Guangzhou, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Wang
authors.affiliation: College of Animal Science & National Engineering Center for Swine Breeding Industry, South China Agriculture University, Guangzhou, China.
authors.forename: Zhi-Wei
authors.initials: ZW
authors.lastname: Wu
authors.affiliation: Institute of Animal Health, Guangdong Academy of Agriculture Sciences, Guangzhou, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Li
authors.affiliation: College of Animal Science & National Engineering Center for Swine Breeding Industry, South China Agriculture University, Guangzhou, China.|Xinyang Animal Disease Prevention and Control Engineering Research Center, Xinyang College of Agriculture and Forestry, Xinyang, China.
authors.forename: Jian-Guo
authors.initials: JG
authors.lastname: Dong
authors.affiliation: College of Animal Science & National Engineering Center for Swine Breeding Industry, South China Agriculture University, Guangzhou, China.
authors.forename: Le-Yi
authors.initials: LY
authors.lastname: Zhang
authors.affiliation: College of Animal Science & National Engineering Center for Swine Breeding Industry, South China Agriculture University, Guangzhou, China.
authors.forename: Peng-Shuai
authors.initials: PS
authors.lastname: Liang
authors.affiliation: College of Animal Science & National Engineering Center for Swine Breeding Industry, South China Agriculture University, Guangzhou, China.
authors.forename: Yan-Ling
authors.initials: YL
authors.lastname: Liu
authors.affiliation: College of Animal Science & National Engineering Center for Swine Breeding Industry, South China Agriculture University, Guangzhou, China.
authors.forename: Ya-Hua
authors.initials: YH
authors.lastname: Zhao
authors.affiliation: College of Animal Science & National Engineering Center for Swine Breeding Industry, South China Agriculture University, Guangzhou, China.|Institute of Animal Health, Guangdong Academy of Agriculture Sciences, Guangzhou, China.
authors.forename: Chang-Xu
authors.identifier: http://orcid.org/0000-0001-5311-0439
authors.initials: CX
authors.lastname: Song
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D020543:Proteome; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D057788:Staphylococcus hyicus / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D020543:Proteome