title: Molecular inhibition mechanisms of cell migration and invasion by coix polysaccharides in A549 NSCLC cells via targeting S100A4.
abstract: S100 calcium binding protein A4 (S100A4) promotes extracellular signal transduction, intercellular adhesion, motility and mobility. Different extracts from Coix lachryma-jobi have been used for the treatment of various types of cancer in Asia. In our previous study, the polysaccharide fraction extact, CP1, induced cell apoptosis of non‑small cell lung cancer cells. In the current study, CP1 inhibited migration and invasion of A549 cells in a scratch wound healing assay and matrigel invasion assay, respectively. Furthermore, reverse transcription‑polymerase chain reaction and western blotting demonstrated that CP1 downregulated the gene and protein expression levels of S100A4. In silico docking analysis demonstrated that polysaccharides may not interfere with dimerization, whereas, the affinity of polysaccharides for an S100A4‑NMIIA pocket was margnially greater than at the dimerization sites. Thus, CP1 inhibited A549 cell migration and invasion potentially via downregulation of S100A4, and may also interact with the binding site of S100A4‑NMIIA, which indicated that CP1 has potential as an alternative cancer chemotherapeutic by targeting S100A4.
authors.affiliation: School of Food and Pharmacy, Zhejiang Ocean University, Zhoushan, Zhejiang 316022, P.R. China.
authors.forename: Cheng
authors.initials: C
authors.lastname: Luo
authors.affiliation: School of Food and Pharmacy, Zhejiang Ocean University, Zhoushan, Zhejiang 316022, P.R. China.
authors.forename: Xin
authors.initials: X
authors.lastname: Wang
authors.affiliation: School of Food and Pharmacy, Zhejiang Ocean University, Zhoushan, Zhejiang 316022, P.R. China.
authors.forename: Can
authors.initials: C
authors.lastname: An
authors.affiliation: Department of Food Science and Technology, Hung Kuang University, Taichung 43302, Taiwan, R.O.C.
authors.forename: Chin-Fa
authors.initials: CF
authors.lastname: Hwang
authors.affiliation: School of Food and Pharmacy, Zhejiang Ocean University, Zhoushan, Zhejiang 316022, P.R. China.
authors.forename: Wenhua
authors.initials: W
authors.lastname: Miao
authors.affiliation: Division of Pharmacognosy, Department of Medicinal Chemistry, Biomedical Center, University of Uppsala, 75123 Uppsala, Sweden.
authors.forename: Lu
authors.initials: L
authors.lastname: Yang
authors.affiliation: Department of Food and Environmental Sciences, Division of Food Chemistry, University of Helsinki, F‑00014 Helsinki, Finland.
authors.forename: Maonian
authors.initials: M
authors.lastname: Xu
authors.affiliation: Department of Medicine, Beth Israel Deaconess Medical Center, Harvard University, Boston, MA 02215, USA.
authors.forename: Aiping
authors.initials: A
authors.lastname: Bai
authors.affiliation: School of Food and Pharmacy, Zhejiang Ocean University, Zhoushan, Zhejiang 316022, P.R. China.
authors.forename: Shanggui
authors.initials: S
authors.lastname: Deng
mesh_terms: D000072283:A549 Cells; D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000494:pharmacology*; D002289:Carcinoma, Non-Small-Cell Lung / Q000188:drug therapy* / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects*; D031742:Coix / Q000737:chemistry*; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D006801:Humans; D008168:Lung / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D008175:Lung Neoplasms / Q000188:drug therapy* / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D062105:Molecular Docking Simulation; D009361:Neoplasm Invasiveness / Q000235:genetics / Q000473:pathology / Q000517:prevention & control; D011134:Polysaccharides / Q000737:chemistry / Q000494:pharmacology*; D000071999:S100 Calcium-Binding Protein A4 / Q000032:analysis / Q000235:genetics / Q000378:metabolism*
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D011134:Polysaccharides; D000071999:S100 Calcium-Binding Protein A4