title: Transcriptome Profiling Analysis of Wolf Spider Pardosa pseudoannulata (Araneae: Lycosidae) after Cadmium Exposure.
abstract: Pardosa pseudoannulatais one of the most common wandering spiders in agricultural fields and a potentially good bioindicator for heavy metal contamination. However, little is known about the mechanisms by which spiders respond to heavy metals at the molecular level. In the present study, high-throughput transcriptome sequencing was employed to characterize the de novo transcriptome of the spiders and to identify differentially expressed genes (DEGs) after cadmium exposure. We obtained 60,489 assembled unigenes, 18,773 of which were annotated in the public databases. A total of 2939 and 2491 DEGs were detected between the libraries of two Cd-treated groups and the control. Functional enrichment analysis revealed that metabolism processes and digestive system function were predominately enriched in response to Cd stress. At the cellular and molecular levels, significantly enriched pathways in lysosomes and phagosomes as well as replication, recombination and repair demonstrated that oxidative damage resulted from Cd exposure. Based on the selected DEGs, certain critical genes involved in defence and detoxification were analysed. These results may elucidate the molecular mechanisms underlying spiders' responses to heavy metal stress.
authors.affiliation: Hubei Collaborative Innovation Center for Green Transformation of Bio-Resources, College of Life Sciences, Hubei University, Wuhan 430062, China. lcc386@163.com.|Hubei Key Laboratory of Quality Control of Characteristic Fruits and Vegetables, Hubei Engineering University, Xiaogan 432000, China. lcc386@163.com.
authors.forename: Chang-Chun
authors.initials: CC
authors.lastname: Li
authors.affiliation: Hubei Key Laboratory of Quality Control of Characteristic Fruits and Vegetables, Hubei Engineering University, Xiaogan 432000, China. wangyong@hbeu.edu.cn.
authors.forename: Yong
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Hubei Key Laboratory of Quality Control of Characteristic Fruits and Vegetables, Hubei Engineering University, Xiaogan 432000, China. lgy64662@sohu.com.
authors.forename: Guo-Yuan
authors.initials: GY
authors.lastname: Li
authors.affiliation: Hubei Collaborative Innovation Center for Green Transformation of Bio-Resources, College of Life Sciences, Hubei University, Wuhan 430062, China. pearlyyl@yahoo.cn.
authors.forename: Yue-Li
authors.initials: YL
authors.lastname: Yun
authors.affiliation: Hubei Key Laboratory of Quality Control of Characteristic Fruits and Vegetables, Hubei Engineering University, Xiaogan 432000, China. dyj5925@sina.com.
authors.forename: Yu-Jun
authors.initials: YJ
authors.lastname: Dai
authors.affiliation: Hubei Collaborative Innovation Center for Green Transformation of Bio-Resources, College of Life Sciences, Hubei University, Wuhan 430062, China. panhui_hb@tom.com.
authors.forename: Jian
authors.initials: J
authors.lastname: Chen
authors.affiliation: Hubei Collaborative Innovation Center for Green Transformation of Bio-Resources, College of Life Sciences, Hubei University, Wuhan 430062, China. pengyu@hubu.edu.cn.
authors.forename: Yu
authors.initials: Y
authors.lastname: Peng
mesh_terms: D000818:Animals; D002104:Cadmium / Q000633:toxicity*; D020869:Gene Expression Profiling / Q000379:methods*; D063990:Gene Ontology; D059014:High-Throughput Nucleotide Sequencing; D008658:Inactivation, Metabolic / Q000187:drug effects / Q000235:genetics; D058977:Molecular Sequence Annotation; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D015203:Reproducibility of Results; D013112:Spiders / Q000187:drug effects* / Q000235:genetics*; D059467:Transcriptome / Q000235:genetics
chemical_list: D012333:RNA, Messenger; D002104:Cadmium
keywords: P. pseudoannulata; RT-qPCR; cadmium; transcriptome