title: Prediction of cancer progression in a group of 73 gastric cancer patients by circulating cell-free DNA.
abstract: Circulating cell-free DNA (ccf-DNA) in plasma may contain both specific and non-specific of tumor markers. The concentration and integrity of ccf-DNA may be clinical useful for detecting and predicting cancer progression.
  
  
  
  Plasma samples from 40 healthy controls and 73 patients with gastric cancers (two stage 0, 17 stage I, 11 stage II, 33 stage III, and 10 stage IV according to American Joint Committee on Cancer stage) were assessed respectively. qPCR targeting the Alu repeats was performed using two different sets of primers amplifying the long and short segments. DNA integrity was calculated as a ratio of the long to the short fragments of Alu repeats.
  
  
  
  Plasma DNA concentration was significantly higher in patients with stage III and IV gastric cancers than in healthy controls (p = 0.028 and 0.029 respectively). The receiver operating characteristic (ROC) curve for discriminating patients with stage III and IV gastric cancers from healthy controls had an area under the curve (AUC) of 0.744 (95% CI, 0.64 to 0.85). Circulating cell-free DNA concentration increased within 21 days following surgery and dropped by 3 months after surgery.
  
  
  
  Concentration of ccf-DNA is a promising molecular marker for assessing gastric cancer progression.
  
  
  
  Current Controlled Trials ChiCTR-DDT-12002848 , 8 October 2012.
authors.affiliation: Department of Oncology, The Second Affiliated Hospital of Soochow University, Suzhou, 215004, China.
authors.forename: Wang-Yang
authors.initials: WY
authors.lastname: Pu
authors.affiliation: Department of Obstetrics and Gynecology, The Second Affiliated Hospital of Soochow University, Suzhou, 215004, China.
authors.forename: Rong
authors.initials: R
authors.lastname: Zhang
authors.affiliation: Molecular Medicine Center, The Second Affiliated Hospital of Soochow University, Suzhou, 215004, China.
authors.forename: Li
authors.initials: L
authors.lastname: Xiao
authors.affiliation: Department of General Surgery, The Second Affiliated Hospital of Soochow University, Suzhou, 215004, China.
authors.forename: Yong-You
authors.initials: YY
authors.lastname: Wu
authors.affiliation: Department of General Surgery, The Second Affiliated Hospital of Soochow University, Suzhou, 215004, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Gong
authors.affiliation: Department of General Surgery, The Second Affiliated Hospital of Soochow University, Suzhou, 215004, China.
authors.forename: Xiao-Dong
authors.initials: XD
authors.lastname: Lv
authors.affiliation: Department of General Surgery, The Second Affiliated Hospital of Soochow University, Suzhou, 215004, China.
authors.forename: Feng-Yun
authors.initials: FY
authors.lastname: Zhong
authors.affiliation: Department of Oncology, The Second Affiliated Hospital of Soochow University, Suzhou, 215004, China.
authors.forename: Zhi-Xiang
authors.initials: ZX
authors.lastname: Zhuang
authors.affiliation: Department of Interventional Radiology, The Second Affiliated Hospital of Soochow University, Suzhou, 215004, China.
authors.forename: Xu-Ming
authors.initials: XM
authors.lastname: Bai
authors.affiliation: Department of Molecular Diagnostics and Biopharmaceutics, College of Pharmaceutical Science, Soochow University, Suzhou, 215004, China. likai@suda.edu.cn.
authors.forename: Kai
authors.initials: K
authors.lastname: Li
authors.affiliation: Department of General Surgery, The Second Affiliated Hospital of Soochow University, Suzhou, 215004, China. xingcg@126.com.
authors.forename: Chun-Gen
authors.initials: CG
authors.lastname: Xing
mesh_terms: D000328:Adult; D000368:Aged; D016022:Case-Control Studies; D004247:DNA / Q000097:blood / Q000235:genetics*; D018450:Disease Progression; D005260:Female; D005500:Follow-Up Studies; D006801:Humans; D008113:Liver Neoplasms / Q000097:blood / Q000235:genetics / Q000556:secondary* / Q000601:surgery; D008175:Lung Neoplasms / Q000097:blood / Q000235:genetics / Q000556:secondary* / Q000601:surgery; D008207:Lymphatic Metastasis; D008297:Male; D008875:Middle Aged; D009361:Neoplasm Invasiveness; D009367:Neoplasm Staging; D010534:Peritoneal Neoplasms / Q000097:blood / Q000235:genetics / Q000556:secondary* / Q000601:surgery; D016133:Polymerase Chain Reaction; D011379:Prognosis; D013274:Stomach Neoplasms / Q000097:blood / Q000235:genetics / Q000473:pathology* / Q000601:surgery
chemical_list: D004247:DNA
keywords: Alu-qPCR; Circulating cell-free DNA; DNA integrity; Gastric cancer