title: Effects of different components of the extracellular matrix on endothelialization.
abstract: The endothelialization of cardiovascular prostheses is known to improve their haemocompatibility. As such body-foreign materials often do not endothelialize spontaneously. A lot of in vitro studies are ongoing how endothelialization of biomaterials can be improved. In this study the influence of different components of a tissue-typical extracellular matrix (ECM) like laminin, fibronectin or gelatin on the formation of an endothelial cell monolayer and on the shear resistance of adherent cells on these substrates was studied.The study revealed that the density of human venous endothelial cells (HUVEC) monolayers differed markedly between cells grown on a natural ECM and cells grown on singularized components of an ECM (p < 0.001). Only HUVEC grown on laminin showed similar densities and a stress fiber pattern comparable to HUVEC grown on the ECM. HUVEC grown on gelatin- or fibronectin-coated coverslips were less firmly attached to the substrate; frequently individual HUVEC and even groups of cells detached.Concluding it seems that coating of implants with laminin supports the formation of shear resistant endothelial cell (EC) monolayer - superior to other ECM components.
authors.affiliation: Institute of Biomaterial Science and Berlin-Brandenburg Center for Regenerative Therapies, Helmholtz-Zentrum Geesthacht, Teltow, Germany.
authors.forename: A
authors.initials: A
authors.lastname: Krüger-Genge
authors.affiliation: Abteilung Biomaterialien, Zentralinstitut für Biomedizinische Technik, Universität Ulm, Ulm, Germany.
authors.forename: R
authors.initials: R
authors.lastname: Fuhrmann
authors.affiliation: Institute of Biomaterial Science and Berlin-Brandenburg Center for Regenerative Therapies, Helmholtz-Zentrum Geesthacht, Teltow, Germany.
authors.forename: F
authors.initials: F
authors.lastname: Jung
authors.affiliation: Abteilung Biomaterialien, Zentralinstitut für Biomedizinische Technik, Universität Ulm, Ulm, Germany.
authors.forename: R P
authors.initials: RP
authors.lastname: Franke
mesh_terms: D002478:Cells, Cultured; D042783:Endothelial Cells / Q000187:drug effects*; D004730:Endothelium, Vascular / Q000187:drug effects*; D005109:Extracellular Matrix / Q000187:drug effects*; D006801:Humans
keywords: HUVEC; extracellular matrix; fibronectin; gelatin; laminin