title: Novel Platform of Cardiomyocyte Culture and Coculture via Fibroblast-Derived Matrix-Coupled Aligned Electrospun Nanofiber.
abstract: For cardiac tissue engineering, much attention has been given to the artificial cardiac microenvironment in which anisotropic design of scaffold and extracellular matrix (ECM) are the major cues. Here we propose poly(l-lactide-co-caprolactone) and fibroblast-derived ECM (PLCL/FDM), a hybrid scaffold that combines aligned electrospun PLCL fibers and FDM. Fibroblasts were grown on the PLCL fibers for 5-7 days and subsequently decellularized to produce PLCL/FDM. Various analyses confirmed aligned, FDM-deposited PLCL fibers. Compared to fibronectin (FN)-coated electrospun PLCL fibers (control), H9c2 cardiomyoblast differentiation was significantly effective, and neonatal rat cardiomyocyte (CM) phenotype and maturation was improved on PLCL/FDM. Moreover, a coculture platform was created using multilayer PLCL/FDM in which two different cells make indirect or direct cell-cell contacts. Such coculture platforms demonstrate their feasibility in terms of higher cell viability, efficiency of target cell harvest (>95% in noncontact; 85% in contact mode), and molecular diffusion through the PLCL/FDM layer. Coculture of primary CMs and fibroblasts exhibited much better CM phenotype and improvement of CM maturity upon either direct or indirect interactions, compared to the conventional coculture systems (transwell insert and tissue culture plate (TCP)). Taken together, our platform should be very useful and have significant contributions in investigating some scientific or practical issues of crosstalks between multiple cell types.
authors.affiliation: Department of Biomedical Engineering, Korea University of Science and Technology (UST) , Daejon 34113, Republic of Korea.
authors.forename: Muhammad
authors.initials: M
authors.lastname: Suhaeri
authors.affiliation: Department of Biomedical Engineering, Korea University of Science and Technology (UST) , Daejon 34113, Republic of Korea.
authors.forename: Ramesh
authors.initials: R
authors.lastname: Subbiah
authors.forename: Su-Hyun
authors.initials: SH
authors.lastname: Kim
authors.forename: Chong-Hyun
authors.initials: CH
authors.lastname: Kim
authors.forename: Seung Ja
authors.initials: SJ
authors.lastname: Oh
authors.affiliation: Department of Biomedical Engineering, Korea University of Science and Technology (UST) , Daejon 34113, Republic of Korea.
authors.forename: Sang-Heon
authors.initials: SH
authors.lastname: Kim
authors.affiliation: Department of Biomedical Engineering, Korea University of Science and Technology (UST) , Daejon 34113, Republic of Korea.
authors.forename: Kwideok
authors.identifier: 0000-0001-8519-8316
authors.initials: K
authors.lastname: Park
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D018920:Coculture Techniques; D005347:Fibroblasts; D032383:Myocytes, Cardiac; D057139:Nanofibers; D011091:Polyesters; D051381:Rats; D023822:Tissue Engineering; D054457:Tissue Scaffolds
chemical_list: D011091:Polyesters
keywords: H9c2 cardiomyoblast; cardiac tissue engineering; coculture platform; fibroblast-derived ECM; neonatal rat cardiomyocytes