title: Oxidative Stress and Biochemical Markers of Endothelial Dysfunction and Organ Damage under Conditions of Experimental Nonferrous Metal Intoxication.
abstract: Chronic nickel intoxication caused by parenteral nickel chloride administration (0.5 mg/kg of body weight) to Wistar rats led to ROS generation inducing LPO in erythrocyte membranes and homogenates of renal, liver, and myocardial tissue. Superoxide dismutase (SOD) activity was inhibited, while catalase activity and ceruloplasmin concentration increased. LPO and its products disrupted nitric oxide production and reduced its bioavailability, which led to the development of endothelial dysfunction and impaired microcirculatory hemodynamics. At the same time, damage of cytoplasmic membranes of internal organs (kidney, liver, and myocardium) was revealed, which was seen from reduced Na+, K+-ATPase activity in homogenates of these organs and increased serum activity of organ-specific (ALT, AST, and γ-glutamyl transpeptidase) and excretory (alkaline phosphatase) enzymes.
authors.affiliation: Institute of Biomedical Research, Vladikavkaz Research Center of Russian Academy of Sciences, Vladikavkaz, Russia. patbiochem@mail.ru.
authors.forename: F S
authors.initials: FS
authors.lastname: Dzugkoeva
authors.affiliation: Institute of Biomedical Research, Vladikavkaz Research Center of Russian Academy of Sciences, Vladikavkaz, Russia.
authors.forename: I V
authors.initials: IV
authors.lastname: Mozhaeva
authors.affiliation: Institute of Biomedical Research, Vladikavkaz Research Center of Russian Academy of Sciences, Vladikavkaz, Russia.
authors.forename: S G
authors.initials: SG
authors.lastname: Dzugkoev
authors.affiliation: Institute of Biomedical Research, Vladikavkaz Research Center of Russian Academy of Sciences, Vladikavkaz, Russia.
authors.forename: O I
authors.initials: OI
authors.lastname: Margieva
authors.affiliation: Institute of Biomedical Research, Vladikavkaz Research Center of Russian Academy of Sciences, Vladikavkaz, Russia.
authors.forename: A I
authors.initials: AI
authors.lastname: Tedtoeva
authors.affiliation: Institute of Biomedical Research, Vladikavkaz Research Center of Russian Academy of Sciences, Vladikavkaz, Russia.
authors.forename: M A
authors.initials: MA
authors.lastname: Otiev
mesh_terms: D000469:Alkaline Phosphatase / Q000097:blood; D000818:Animals; D002374:Catalase / Q000097:blood; D002570:Ceruloplasmin / Q000378:metabolism; D056486:Chemical and Drug Induced Liver Injury / Q000378:metabolism* / Q000473:pathology; D004912:Erythrocytes / Q000737:chemistry / Q000187:drug effects; D005982:Glutathione Transferase / Q000097:blood; D007668:Kidney / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology; D015227:Lipid Peroxidation / Q000187:drug effects; D008099:Liver / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology; D008297:Male; D008315:Malondialdehyde / Q000097:blood; D009206:Myocardium / Q000378:metabolism* / Q000473:pathology; D009532:Nickel / Q000633:toxicity*; D009569:Nitric Oxide / Q000097:blood; D018384:Oxidative Stress; D051381:Rats; D017208:Rats, Wistar; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism; D013482:Superoxide Dismutase / Q000097:blood; D005723:gamma-Glutamyltransferase / Q000097:blood
chemical_list: D009569:Nitric Oxide; D008315:Malondialdehyde; C022838:nickel chloride; D009532:Nickel; D002374:Catalase; D013482:Superoxide Dismutase; D002570:Ceruloplasmin; D005723:gamma-Glutamyltransferase; D005982:Glutathione Transferase; D000469:Alkaline Phosphatase; D000254:Sodium-Potassium-Exchanging ATPase
keywords: antioxidant system; endothelial dysfunction; lipid peroxidation; nickel chloride; nitric oxide