title: Analysis of the Serum Lipid Profile in Polypoidal Choroidal Vasculopathy.
abstract: Polypoidal choroidal vasculopathy (PCV), the predominant subtype of neovascular age-related macular degeneration in the Asian population, is associated with genetic polymorphism of lipid metabolism. In this study, we performed the untargeted lipidomics approach of ultra-performance liquid chromatography coupled with mass spectrometry (UPLC-MS) to reveal the potential discriminating lipid profile of PCV patients in serum (21 PCV patients and 19 age-matched controls). Unsupervised principal component, supervised orthogonal partial least squares analysis, correlation analysis, and heatmap analysis were performed with the data obtained by UPLC-MS. Forty-one discriminating metabolites were identified. Receiver operating characteristic curve analysis, pathway analysis and functional analysis were performed subsequently, and platelet-activating factor (PAF) was further selected as the key indicator of the distinct lipid metabolism in PCV patients. Finally, the serum level of PAF was validated by enzyme-linked immunosorbent assay, which is significantly higher in PCV patients compared to controls (65 PCV patients and 63 age-matched controls, p < 0.0001), consistent with the UPLC-MS analysis. Our results suggested that PAF is considered as the major indicator of the distinct lipid metabolism in PCV patients.
authors.affiliation: State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou, China.
authors.forename: Miaoling
authors.initials: M
authors.lastname: Li
authors.affiliation: State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou, China.
authors.forename: Xiongze
authors.initials: X
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou, China.
authors.forename: Nanying
authors.initials: N
authors.lastname: Liao
authors.affiliation: State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou, China.
authors.forename: Baikang
authors.initials: B
authors.lastname: Ye
authors.affiliation: State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou, China.
authors.forename: Yuting
authors.initials: Y
authors.lastname: Peng
authors.affiliation: State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou, China.
authors.forename: Yuying
authors.initials: Y
authors.lastname: Ji
authors.affiliation: State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Wen
mesh_terms: D000368:Aged; D015415:Biomarkers / Q000097:blood; D016022:Case-Control Studies; D002829:Choroid / Q000098:blood supply / Q000378:metabolism / Q000473:pathology; D020256:Choroidal Neovascularization / Q000097:blood / Q000175:diagnosis* / Q000473:pathology; D005260:Female; D015870:Gene Expression; D006801:Humans; D016018:Least-Squares Analysis; D050356:Lipid Metabolism; D008268:Macular Degeneration / Q000097:blood / Q000175:diagnosis* / Q000473:pathology; D008297:Male; D055442:Metabolome; D008875:Middle Aged; D010972:Platelet Activating Factor / Q000235:genetics* / Q000378:metabolism; D025341:Principal Component Analysis; D012372:ROC Curve
chemical_list: D015415:Biomarkers; D010972:Platelet Activating Factor