title: Hormone-sensitive lipase deficiency alters gene expression and cholesterol content of mouse testis.
abstract: Hormone-sensitive lipase-knockout (HSL-/-) mice exhibit azoospermia for unclear reasons. To explore the basis of sterility, we performed the following three experiments. First, HSL protein distribution in the testis was determined. Next, transcriptome analyses were performed on the testes of three experimental groups. Finally, the fatty acid and cholesterol levels in the testes with three different genotypes studied were determined. We found that the HSL protein was present from spermatocyte cells to mature sperm acrosomes in wild-type (HSL+/+) testes. Spermiogenesis ceased at the elongation phase of HSL-/- testes. Transcriptome analysis indicated that genes involved in lipid metabolism, cell membrane, reproduction and inflammation-related processes were disordered in HSL-/- testes. The cholesterol content was significantly higher in HSL-/- than that in HSL+/+ testis. Therefore, gene expression and cholesterol ester content differed in HSL-/- testes compared to other testes, which may explain the sterility of male HSL-/- mice.
authors.affiliation: College of Life SciencesCapital Normal University, Beijing, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Wang
authors.affiliation: College of Life SciencesCapital Normal University, Beijing, China.
authors.forename: Zheng
authors.initials: Z
authors.lastname: Chen
authors.affiliation: College of Life SciencesCapital Normal University, Beijing, China.
authors.forename: Xiaofang
authors.initials: X
authors.lastname: Ren
authors.affiliation: College of Life SciencesCapital Normal University, Beijing, China.
authors.forename: Ye
authors.initials: Y
authors.lastname: Tian
authors.affiliation: College of Life SciencesCapital Normal University, Beijing, China.
authors.forename: Fucheng
authors.initials: F
authors.lastname: Wang
authors.affiliation: College of Life SciencesCapital Normal University, Beijing, China.
authors.forename: Chao
authors.initials: C
authors.lastname: Liu
authors.affiliation: College of Life SciencesCapital Normal University, Beijing, China.
authors.forename: Pengcheng
authors.initials: P
authors.lastname: Jin
authors.affiliation: College of Life SciencesCapital Normal University, Beijing, China.
authors.forename: Zongyue
authors.initials: Z
authors.lastname: Li
authors.affiliation: College of Life SciencesCapital Normal University, Beijing, China.
authors.forename: Feixiong
authors.initials: F
authors.lastname: Zhang
authors.affiliation: College of Life SciencesCapital Normal University, Beijing, China baochang@cnu.edu.cn.
authors.forename: Baochang
authors.initials: B
authors.lastname: Zhu
mesh_terms: D000818:Animals; D053713:Azoospermia / Q000209:etiology / Q000235:genetics; D002784:Cholesterol / Q000032:analysis; D002788:Cholesterol Esters / Q000032:analysis; D005227:Fatty Acids / Q000032:analysis; D005260:Female; D015870:Gene Expression; D020869:Gene Expression Profiling / Q000662:veterinary; D005838:Genotype; D006579:Heterozygote; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D013091:Spermatogenesis / Q000235:genetics; D013094:Spermatozoa / Q000737:chemistry; D002787:Sterol Esterase / Q000032:analysis / Q000172:deficiency* / Q000502:physiology; D013737:Testis / Q000201:enzymology
chemical_list: D002788:Cholesterol Esters; D005227:Fatty Acids; D002784:Cholesterol; D002787:Sterol Esterase