title: Lipidoid mRNA Nanoparticles for Myocardial Delivery in Rodents.
abstract: An area of active research in the field of cardiac gene therapy aims to achieve high transfection efficiency without eliciting immune or inflammatory reactions. Nanomedicine offers an attractive alternative to traditional viral delivery vehicles because nanoparticle technology can enable safer and more controlled delivery of therapeutic agents. Here we describe the use of lipidoid nanoparticles for delivery of modified mRNA (modRNA) to the myocardium in vivo, with a focus on rodent models that represent a first step toward preclinical studies. Three major procedures are discussed in this chapter: (1) preparation of lipid modRNA nanoparticles, (2) intramyocardial delivery of the lipid modRNA nanoparticles by direct injection with an open chest technique in rats, and (3) intracoronary delivery of the lipid modRNA nanoparticles with open chest and temporary aortic cross clamping in rats.
authors.affiliation: Cardiovascular Research Center, Icahn School of Medicine at Mount Sinai, One Gustave L. Levy Place, Box 1030, New York, NY, 10029, USA.
authors.forename: Irene C
authors.initials: IC
authors.lastname: Turnbull
authors.affiliation: Stanford Graduate School of Business, Stanford, CA, USA.
authors.forename: Ahmed A
authors.initials: AA
authors.lastname: Eltoukhy
authors.affiliation: Department of Chemical Engineering, Institute for Medical Engineering and Science, David H Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, MA, USA.
authors.forename: Daniel G
authors.initials: DG
authors.lastname: Anderson
authors.affiliation: Cardiovascular Research Center, Icahn School of Medicine at Mount Sinai, One Gustave L. Levy Place, Box 1030, New York, NY, 10029, USA. kevin.costa@mssm.edu.
authors.forename: Kevin D
authors.initials: KD
authors.lastname: Costa
mesh_terms: D000818:Animals; D018014:Gene Transfer Techniques; D007267:Injections; D008055:Lipids / Q000737:chemistry*; D009206:Myocardium / Q000378:metabolism*; D053758:Nanoparticles / Q000008:administration & dosage*; D012333:RNA, Messenger / Q000008:administration & dosage*; D051381:Rats
chemical_list: D008055:Lipids; D012333:RNA, Messenger
keywords: Intracoronary injection; Intramyocardial injection; Lipid nanoparticles; Lipidoid; Modified mRNA