title: Multicolor Karyotyping and Fluorescence In Situ Hybridization-Banding (MCB/mBAND).
abstract: Multicolor fluorescence in situ hybridization (mFISH) approaches are routine applications in tumor as well as clinical cytogenetics nowadays. The first approach when thinking about mFISH is multicolor karyotyping using human whole chromosome paints as probes; this can be achieved by narrow-band filter-based multiplex-FISH (M-FISH) or interferometer/spectroscopy-based spectral karyotyping (SKY). Besides, various FISH-based banding approaches were reported in the literature, including multicolor banding (MCB/mBAND) the latter being evaluated by narrow-band filters, and using specific software. Here, we describe the combined application of multicolor karyotyping and MCB/mBAND for the characterization of simple and complex acquired chromosomal changes in cancer cytogenetics.
authors.affiliation: Jena University Hospital, Friedrich Schiller University, Institute of Human Genetics, Kollegiengasse 10, D-07743, Jena, Germany. Thomas.Liehr@med.uni-jena.de.
authors.forename: Thomas
authors.initials: T
authors.lastname: Liehr
authors.affiliation: Jena University Hospital, Friedrich Schiller University, Institute of Human Genetics, Kollegiengasse 10, D-07743, Jena, Germany.
authors.forename: Moneeb A K
authors.initials: MA
authors.lastname: Othman
authors.affiliation: Jena University Hospital, Friedrich Schiller University, Institute of Human Genetics, Kollegiengasse 10, D-07743, Jena, Germany.
authors.forename: Katharina
authors.initials: K
authors.lastname: Rittscher
mesh_terms: D002869:Chromosome Aberrations; D002871:Chromosome Banding; D020223:Chromosome Painting; D019295:Computational Biology / Q000379:methods; D015342:DNA Probes; D006801:Humans; D007091:Image Processing, Computer-Assisted; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D032681:Spectral Karyotyping / Q000379:methods*
chemical_list: D015342:DNA Probes
keywords: Multicolor banding (MCB/mBAND); Multicolor fluorescence in situ hybridization (mFISH); Multicolor karyotyping; Multiplex-FISH (M-FISH); Spectral karyotyping (SKY)