title: Effect of tempol and tempol plus catalase on intra-renal haemodynamics in spontaneously hypertensive stroke-prone (SHSP) and Wistar rats.
abstract: Vasoconstriction within the renal medulla contributes to the development of hypertension. This study investigated the role of reactive oxygen species (ROS) in regulating renal medullary and cortical blood perfusion (MBP and CBP respectively) in both stroke-prone spontaneously hypertensive rats (SHRSP) and Wistar rats. CBP and MBP were measured using a laser-Doppler flow meter before and after intra-renal infusion of tempol, the superoxide dismutase (SOD) mimetic or tempol plus catalase, the hydrogen peroxide-degrading enzyme. Tempol infusion significantly elevated blood perfusion within the renal medulla (MBP) in both SHRSP (by 43 ± 7%, P < 0.001) and Wistar rats (by 17 ± 2%, P < 0.05) but the magnitude of the increase was significantly greater in the SHRSP (P < 0.01). When the enzyme catalase and tempol were co-infused, MBP was again significantly increased in SHRSP (by 57 ± 6%, P < 0.001) and Wistar rats (by 33 ± 6%, P < 0.001), with a significantly greater increase in perfusion being induced in the SHRSP relative to the Wistar rats (P < 0.01). Notably, this increase was significantly greater than in those animals infused with tempol alone (P < 0.01). These results suggest that ROS plays a proportionally greater role in reducing renal vascular compliance, particularly within the renal medulla, in normotensive and hypertensive animals, with effects being greater in the hypertensive animals. This supports the hypothesis that SHRSP renal vasculature might be subjected to elevated level of oxidative stress relative to normotensive animals.
authors.affiliation: Department of Physiology, College of Medicine, King Saud University, Riyadh, Kingdom of Saudi Arabia. aahmeda@ksu.edu.sa.
authors.forename: Ahmad F
authors.initials: AF
authors.lastname: Ahmeda
authors.affiliation: Department of Physiology, University College Cork, Cork, Republic of, Ireland.
authors.forename: Mark G
authors.initials: MG
authors.lastname: Rae
authors.affiliation: Department of Physiology, College of Medicine, King Saud University, Riyadh, Kingdom of Saudi Arabia.
authors.forename: Mohammed F
authors.initials: MF
authors.lastname: Al Otaibi
authors.affiliation: College of Dentistry, Princess Nourah bint Abdulrahman University, Riyadh, Kingdom of Saudi Arabia.
authors.forename: Lamyia M
authors.initials: LM
authors.lastname: Anweigi
authors.affiliation: Department of Physiology, University College Cork, Cork, Republic of, Ireland.
authors.forename: Edward J
authors.initials: EJ
authors.lastname: Johns
mesh_terms: D000818:Animals; D000959:Antihypertensive Agents / Q000008:administration & dosage / Q000009:adverse effects / Q000627:therapeutic use*; D000975:Antioxidants / Q000008:administration & dosage / Q000009:adverse effects / Q000627:therapeutic use*; D002374:Catalase / Q000008:administration & dosage / Q000009:adverse effects / Q000627:therapeutic use*; D002417:Cattle; D003497:Cyclic N-Oxides / Q000008:administration & dosage / Q000009:adverse effects / Q000627:therapeutic use*; D004359:Drug Therapy, Combination / Q000009:adverse effects; D006973:Hypertension / Q000188:drug therapy* / Q000378:metabolism / Q000503:physiopathology; D055103:Hypodermoclysis; D007668:Kidney / Q000098:blood supply / Q000187:drug effects* / Q000378:metabolism / Q000503:physiopathology; D007679:Kidney Medulla / Q000098:blood supply / Q000187:drug effects / Q000378:metabolism / Q000503:physiopathology; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects; D011918:Rats, Inbred SHR; D017208:Rats, Wistar; D017382:Reactive Oxygen Species / Q000378:metabolism; D012079:Renal Circulation / Q000187:drug effects*; D013113:Spin Labels; D020521:Stroke / Q000209:etiology / Q000517:prevention & control; D014655:Vascular Resistance / Q000187:drug effects
chemical_list: D000959:Antihypertensive Agents; D000975:Antioxidants; D003497:Cyclic N-Oxides; D017382:Reactive Oxygen Species; D013113:Spin Labels; D002374:Catalase; C001803:tempol
keywords: Catalase; Reactive oxygen species; Renal blood perfusion; SHRSP; Tempol