title: Hyaluronate-Gold Nanorod/DR5 Antibody Complex for Noninvasive Theranosis of Skin Cancer.
abstract: Noninvasive transdermal delivery is a promising method with distinct advantages including patient compliance over other delivery routes. Here, hyaluronate-gold nanorod/death receptor 5 antibody (HA-AuNR/DR5 Ab) complex was developed for transdermal theranosis of skin cancer. The successful formation of the complex was corroborated by1H nuclear magnetic resonance, UV-vis spectroscopy, dynamic light scattering, zeta potential, and transmission electron microscopy. In vitro biological activity of the complex was verified by ELISA and MTT assay using HCT116 cancer cells. In addition, in vivo photoacoustic imaging and two-photon microscopy clearly visualized the transdermal delivery of HA-AuNR/DR5 Ab complex through the inevitable barrier of stratum corneum in the skin. Furthermore, in vivo antitumor effect on skin cancer model mice was confirmed from statistically significant decrease of tumor-reflecting luciferase expression levels and apoptotic signals in terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay. Taken together, we could confirm the feasibility of HA-AuNR/DR5 Ab complex as a novel theranostic platform for noninvasive transdermal treatment of skin cancers.
authors.forename: Hwiwon
authors.initials: H
authors.lastname: Lee
authors.forename: Jung Ho
authors.initials: JH
authors.lastname: Lee
authors.forename: Jeesu
authors.initials: J
authors.lastname: Kim
authors.forename: Jonghwan
authors.initials: J
authors.lastname: Mun
authors.affiliation: Department of Biochemistry and Molecular Biology and Cancer Research Institute, Seoul National University College of Medicine , Seoul 151-742 Korea.
authors.forename: Junho
authors.initials: J
authors.lastname: Chung
authors.affiliation: Wellman Center for Photomedicine, Harvard Medical School and Massachusetts General Hospital , 65 Landsdowne St. UP-5, Cambridge, Massachusetts 02139, United States.
authors.forename: Heebeom
authors.initials: H
authors.lastname: Koo
authors.forename: Chulhong
authors.initials: C
authors.lastname: Kim
authors.affiliation: Wellman Center for Photomedicine, Harvard Medical School and Massachusetts General Hospital , 65 Landsdowne St. UP-5, Cambridge, Massachusetts 02139, United States.
authors.forename: Seok Hyun
authors.initials: SH
authors.lastname: Yun
authors.forename: Sei Kwang
authors.initials: SK
authors.lastname: Hahn
mesh_terms: D000279:Administration, Cutaneous; D000818:Animals; D006046:Gold; D051379:Mice; D043942:Nanotubes; D012867:Skin; D012878:Skin Neoplasms; D000068936:Theranostic Nanomedicine
chemical_list: D006046:Gold
keywords: antibody; gold nanorod; hyaluronate; skin cancer; transdermal delivery