title: Characterization of iPSCs derived from dermal fibroblasts from a healthy 19year old female.
abstract: Primary fibroblasts from a healthy 19years old female were reprogrammed by transduction of retroviruses OCT4, SOX2, c-MYC and KLF4. iPSCs were characterized by immunocytochemistry, embryonic body-formation, DNA-fingerprint and karyotype analysis and comparative transcriptome analyses with the human embryonic stem cell line H1 revealed a Pearsons correlation coefficient of 0.8952.
authors.affiliation: Institute for Stem Cell Research and Regenerative Medicine, Medical Faculty, Heinrich Heine University, 40225 Düsseldorf, Germany.
authors.forename: Marie-Ann
authors.initials: MA
authors.lastname: Kawala
authors.affiliation: Institute for Stem Cell Research and Regenerative Medicine, Medical Faculty, Heinrich Heine University, 40225 Düsseldorf, Germany.
authors.forename: Martina
authors.initials: M
authors.lastname: Bohndorf
authors.affiliation: Institute for Stem Cell Research and Regenerative Medicine, Medical Faculty, Heinrich Heine University, 40225 Düsseldorf, Germany.
authors.forename: Nina
authors.initials: N
authors.lastname: Graffmann
authors.affiliation: Institute for Stem Cell Research and Regenerative Medicine, Medical Faculty, Heinrich Heine University, 40225 Düsseldorf, Germany.
authors.forename: Wasco
authors.initials: W
authors.lastname: Wruck
authors.affiliation: Institute of Pathology, Medical University of Graz, 8036 Graz, Austria.
authors.forename: Kurt
authors.initials: K
authors.lastname: Zatloukal
authors.affiliation: Institute for Stem Cell Research and Regenerative Medicine, Medical Faculty, Heinrich Heine University, 40225 Düsseldorf, Germany. Electronic address: James.Adjaye@med.uni-duesseldorf.de.
authors.forename: James
authors.initials: J
authors.lastname: Adjaye
mesh_terms: D002454:Cell Differentiation; D002460:Cell Line; D065150:Cellular Reprogramming; D055028:Comparative Genomic Hybridization; D016172:DNA Fingerprinting; D020405:Dermis / Q000166:cytology; D058732:Embryoid Bodies / Q000166:cytology / Q000378:metabolism; D005260:Female; D005347:Fibroblasts / Q000166:cytology; D005822:Genetic Vectors / Q000235:genetics / Q000378:metabolism; D064368:Healthy Volunteers; D000066449:Human Embryonic Stem Cells / Q000166:cytology / Q000378:metabolism; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000166:cytology* / Q000378:metabolism; D059785:Karyotype; D000090062:Kruppel-Like Factor 4; D008856:Microscopy, Fluorescence; D012190:Retroviridae / Q000235:genetics; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D055815:Young Adult
chemical_list: C000715467:KLF4 protein, human; D000090062:Kruppel-Like Factor 4; D014157:Transcription Factors