title: [Effects of apigenin on self-renewal and  uPAR expression in NCI-H446 cell line].
abstract: To investigate the effect of apigenin on self-renewal for sphere-forming cells in human small cell lung cancer cell line NCI-H446 and the underlying mechanisms.  Methods: Sphere-forming cells from NCI-H446 cell line were cultured in stem cell-conditioned culture medium with ultra-low attachment surface plates. The rate of sphere-forming cells in the second passage sphere-forming cells was used to evaluate the inhibitory effects of apigenin on the self-renewal for sphere-forming cells. The protein level of urokinase-type plasminogen activator receptor (uPAR) in spheroids was analyzed by Western blot.  Results: Apigenin signifcantly inhibited the self-renewal of the second passage sphere-forming cells [0, 5.0, 10.0, 20.0 μmol/L apigenin: (18.2±1.9)%, (13.6±1.7)%, (10.6±1.6)%, (6.9±1.3)%, respectively] and down-regulated uPAR expression in a concentration-dependent manner (P<0.05).  Conclusion: Apigenin inhibits the self-renewal capacity of sphere-forming cells in NCI-H446 cells, which may be associated with down-regulation of uPAR expression.
authors.affiliation: Department of Basic Medicine, College of Medicine of Hunan Normal University, Changsha 410013, China.
authors.forename: Ling
authors.initials: L
authors.lastname: Shu
authors.affiliation: Department of Basic Medicine, College of Medicine of Hunan Normal University, Changsha 410013, China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Yuan
authors.affiliation: Department of Basic Medicine, College of Medicine of Hunan Normal University, Changsha 410013, China.
authors.forename: Yinghong
authors.initials: Y
authors.lastname: Cui
authors.affiliation: Department of Basic Medicine, College of Medicine of Hunan Normal University, Changsha 410013, China.
authors.forename: Shuwen
authors.initials: S
authors.lastname: Sun
authors.affiliation: Department of Basic Medicine, College of Medicine of Hunan Normal University, Changsha 410013, China.
authors.forename: A
authors.initials: A
authors.lastname: Chen
authors.affiliation: Department of Basic Medicine, College of Medicine of Hunan Normal University, Changsha 410013, China.
authors.forename: Dan
authors.initials: D
authors.lastname: Chen
authors.affiliation: Department of Basic Medicine, College of Medicine of Hunan Normal University, Changsha 410013, China.
authors.forename: Jianguo
authors.initials: J
authors.lastname: Cao
authors.affiliation: Department of Basic Medicine, College of Medicine of Hunan Normal University, Changsha 410013, China.
authors.forename: Jiansong
authors.initials: J
authors.lastname: Zhang
mesh_terms: D047310:Apigenin / Q000494:pharmacology*; D045744:Cell Line, Tumor; D015536:Down-Regulation / Q000187:drug effects / Q000235:genetics; D006801:Humans; D008175:Lung Neoplasms; D014411:Neoplastic Stem Cells / Q000187:drug effects* / Q000473:pathology / Q000502:physiology*; D011956:Receptors, Cell Surface; D055293:Receptors, Urokinase Plasminogen Activator / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction; D055752:Small Cell Lung Carcinoma / Q000188:drug therapy / Q000473:pathology*; D018874:Spheroids, Cellular / Q000187:drug effects / Q000502:physiology; D013234:Stem Cells
chemical_list: D011956:Receptors, Cell Surface; D055293:Receptors, Urokinase Plasminogen Activator; D047310:Apigenin