title: Simultaneous profiling of 17 steroid hormones for the evaluation of endocrine-disrupting chemicals in H295R cells.
abstract: OBJECTIVE
  There is urgent need to develop a new protocol for the evaluation of chemical substances to potentially interact with the endocrine system and induce numerous pathological issues. The recently validated in vitro screening assay is limited on monitoring two steroid hormones. Methodology & results: The H295R model cell was exposed to seven endocrine disrupting chemicals (EDCs). The levels of 17 steroid hormones in cell extracts were subsequently determined by a quantitative targeted GC/MS/MS method. Through wide coverage, this system managed to capture the effects of exposure to increasing EDCs concentrations in the entire steroidogenic pathways.
  
  
  CONCLUSIONS
  The developed approach could be beneficial for the mechanistic investigation of EDCs.
authors.affiliation: Division of Metabolomics, Research Center for Transomics Medicine, Medical Institute of Bioregulation, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan.
authors.forename: Udi
authors.initials: U
authors.lastname: Jumhawan
authors.affiliation: Division of Metabolomics, Research Center for Transomics Medicine, Medical Institute of Bioregulation, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan.
authors.forename: Toshiyuki
authors.initials: T
authors.lastname: Yamashita
authors.affiliation: Chemicals Assessment & Research Center, Chemicals Evaluation & Research Institute, 1600 Shimotakano, Sugito-machi, Kitakatsushika-gun, Saitama 345-0043, Japan.
authors.forename: Kazuya
authors.initials: K
authors.lastname: Ishida
authors.affiliation: Department of Biotechnology, Graduate School of Engineering, Osaka University, 2-1 Yamadoka, Suita, Osaka 565-0871, Japan.
authors.forename: Eiichiro
authors.initials: E
authors.lastname: Fukusaki
authors.affiliation: Division of Metabolomics, Research Center for Transomics Medicine, Medical Institute of Bioregulation, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan.|Department of Biotechnology, Graduate School of Engineering, Osaka University, 2-1 Yamadoka, Suita, Osaka 565-0871, Japan.
authors.forename: Takeshi
authors.initials: T
authors.lastname: Bamba
mesh_terms: D002460:Cell Line; D052244:Endocrine Disruptors / Q000378:metabolism* / Q000633:toxicity; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D006728:Hormones / Q000378:metabolism*; D006801:Humans; D055442:Metabolome; D013256:Steroids / Q000378:metabolism*; D053719:Tandem Mass Spectrometry / Q000379:methods
chemical_list: D052244:Endocrine Disruptors; D006728:Hormones; D013256:Steroids
keywords: H295R cells; endocrine disrupting chemicals; gas chromatography tandem mass spectrometry; steroid profiling; steroidogenesis