title: Saccharothrix ghardaiensis sp. nov., an actinobacterium isolated from Saharan soil.
abstract: The taxonomic position of a new Saccharothrix strain, designated MB46T, isolated from a Saharan soil sample collected in Mzab region (Ghardaïa province, South Algeria) was established following a polyphasic approach. The novel microorganism has morphological and chemical characteristics typical of the members of the genus Saccharothrix and formed a phyletic line at the periphery of the Saccharothrix espanaensis subcluster in the 16S rRNA gene dendrograms. Results of the 16S rRNA gene sequence comparisons revealed that strain MB46Tshares high degrees of similarity with S. espanaensis DSM 44229T(99.2%), Saccharothrix variisporea DSM 43911T(98.7%) and Saccharothrix texasensis NRRL B-16134T(98.6%). However, the new strain exhibited only 12.5-17.5% DNA relatedness to the neighbouring Saccharothrix spp. On the basis of phenotypic characteristics, 16S rRNA gene sequence comparisons and DNA-DNA hybridizations, strain MB46Tis concluded to represent a novel species of the genus Saccharothrix, for which the name Saccharothrix ghardaiensis sp. nov. (type strain MB46T= DSM 46886T= CECT 9046T) is proposed.
authors.affiliation: Laboratoire de Biologie des Systèmes Microbiens (LBSM), Ecole Normale Supérieure de Kouba, Algiers, Algeria.
authors.forename: Khaoula
authors.initials: K
authors.lastname: Bouznada
authors.affiliation: Laboratoire de Biologie des Systèmes Microbiens (LBSM), Ecole Normale Supérieure de Kouba, Algiers, Algeria.|Département de Biologie, Faculté des Sciences de la Nature et de la Vie et Sciences de la Terre, Université de Ghardaïa, BP 455, 47000, Ghardaïa, Algeria.
authors.forename: Noureddine
authors.initials: N
authors.lastname: Bouras
authors.affiliation: Laboratoire de Biologie des Systèmes Microbiens (LBSM), Ecole Normale Supérieure de Kouba, Algiers, Algeria.
authors.forename: Salim
authors.initials: S
authors.lastname: Mokrane
authors.affiliation: Laboratoire de Biologie des Systèmes Microbiens (LBSM), Ecole Normale Supérieure de Kouba, Algiers, Algeria.
authors.forename: Fawzia
authors.initials: F
authors.lastname: Chaabane Chaouch
authors.affiliation: Laboratoire de Biologie des Systèmes Microbiens (LBSM), Ecole Normale Supérieure de Kouba, Algiers, Algeria.
authors.forename: Abdelghani
authors.initials: A
authors.lastname: Zitouni
authors.affiliation: DSMZ - German Collection of Microorganisms and Cell Cultures, Inhoffenstraße 7B, 38124, Brunswick, Germany.
authors.forename: Gabriele
authors.initials: G
authors.lastname: Pötter
authors.affiliation: DSMZ - German Collection of Microorganisms and Cell Cultures, Inhoffenstraße 7B, 38124, Brunswick, Germany.
authors.forename: Cathrin
authors.initials: C
authors.lastname: Spröer
authors.affiliation: School of Biology, Newcastle University, Ridley Building, Newcastle upon Tyne, NE1 7RU, UK. hans-peter.klenk@ncl.ac.uk.
authors.forename: Hans-Peter
authors.initials: HP
authors.lastname: Klenk
authors.affiliation: Laboratoire de Biologie des Systèmes Microbiens (LBSM), Ecole Normale Supérieure de Kouba, Algiers, Algeria. sabaou@yahoo.fr.
authors.forename: Nasserdine
authors.initials: N
authors.lastname: Sabaou
mesh_terms: D000192:Actinomycetales / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D000352:Africa, Northern; D000462:Algeria; D002473:Cell Wall / Q000737:chemistry; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D003960:Diaminopimelic Acid / Q000032:analysis; D005227:Fatty Acids / Q000032:analysis; D008848:Micromonosporaceae / Q000235:genetics; D010641:Phenotype; D010743:Phospholipids / Q000032:analysis; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012965:Sodium Chloride / Q000378:metabolism; D012987:Soil / Q000737:chemistry; D012988:Soil Microbiology; D013045:Species Specificity; D013696:Temperature; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000032:analysis
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D012987:Soil; D024482:Vitamin K 2; D012965:Sodium Chloride; C073355:menaquinone 9; D003960:Diaminopimelic Acid
keywords: Actinobacteria; Algeria; Polyphasic taxonomy; Saccharothrix; Saccharothrix ghardaiensis sp. nov.; Saharan soil