title: Genetic polymorphisms of IL-18 rs1946518 and IL-1β rs16944 are associated with prognosis and survival of acute myeloid leukemia.
abstract: BACKGROUND
  Though the pathogenesis of AML is still unknown, accumulating evidence revealed that immune response plays a vital part in it. NLRP3 inflammasome as a component of immune system has been found related to several cancers. The single nucleotide polymorphisms (SNPs) of NLRP3 inflammasome genes may be related to pathogenesis and prognosis of AML.
  
  
  RESULTS
  We determined polymorphisms of NLRP3 (rs35829419), CARD8 (rs2043211), IL-1β (rs16944), IL-18 (rs1946518) and NF-κB -94 ins/del ATTG in de novo AML patients to find out whether they play roles in the susceptibility and severity of AML. In our study, 383 AML cases and 300 randomly selected healthy individuals were examined for the polymorphisms and expression of NLRP3 genes. IL-1β (rs16944) polymorphism in different risk AML subgroups was found statistically different, with more GA genotype in favorable-risk cytogenetics group. We also demonstrated that the bone marrow blasts of patients carrying IL-18 (rs1946518) GG or GT genotype were higher than patients of TT genotype. IL-18 plasma level of patients with IL-18 (rs1946518) GT or TT genotype was higher than GG genotype. Moreover, the GT genotype of IL-18 (rs1946518) led to statistically poorer AML-specific survival.
  
  
  CONCLUSIONS
  IL-1β (rs16944) and IL-18 (rs1946518) may be served as potential predictors for AML.
authors.affiliation: Department of Hematology, Qilu Hospital, Shandong University, 107 West Wenhua Rd, Jinan, 250012, People's Republic of China.|Department of Hematology, Zibo Central Hospital, Zibo, Shandong, People's Republic of China.
authors.forename: Hong
authors.initials: H
authors.lastname: Wang
authors.affiliation: Department of Hematology, Qilu Hospital, Shandong University, 107 West Wenhua Rd, Jinan, 250012, People's Republic of China.
authors.forename: Mingqiang
authors.initials: M
authors.lastname: Hua
authors.affiliation: Department of Epidemiology and Biostatistics, School of Public Health, Shandong University, Jinan, People's Republic of China.
authors.forename: Shukang
authors.initials: S
authors.lastname: Wang
authors.affiliation: Department of Hematology, Qilu Hospital, Shandong University, 107 West Wenhua Rd, Jinan, 250012, People's Republic of China.
authors.forename: Jie
authors.initials: J
authors.lastname: Yu
authors.affiliation: Department of Hematology, Qilu Hospital, Shandong University, 107 West Wenhua Rd, Jinan, 250012, People's Republic of China.
authors.forename: Chen
authors.initials: C
authors.lastname: Chen
authors.affiliation: Department of Hematology, Qilu Hospital, Shandong University, 107 West Wenhua Rd, Jinan, 250012, People's Republic of China.
authors.forename: Xueyun
authors.initials: X
authors.lastname: Zhao
authors.affiliation: Department of Hematology, Qilu Hospital, Shandong University, 107 West Wenhua Rd, Jinan, 250012, People's Republic of China.
authors.forename: Chen
authors.initials: C
authors.lastname: Zhang
authors.affiliation: Department of Hematology, Qilu Hospital, Shandong University, 107 West Wenhua Rd, Jinan, 250012, People's Republic of China.
authors.forename: Chaoqin
authors.initials: C
authors.lastname: Zhong
authors.affiliation: Department of Hematology, Qilu Hospital, Shandong University, 107 West Wenhua Rd, Jinan, 250012, People's Republic of China.
authors.forename: Ruiqing
authors.initials: R
authors.lastname: Wang
authors.affiliation: Department of Hematology, Qilu Hospital, Shandong University, 107 West Wenhua Rd, Jinan, 250012, People's Republic of China.
authors.forename: Na
authors.initials: N
authors.lastname: He
authors.affiliation: Department of Hematology, Qilu Hospital, Shandong University, 107 West Wenhua Rd, Jinan, 250012, People's Republic of China.
authors.forename: Ming
authors.initials: M
authors.lastname: Hou
authors.affiliation: Department of Hematology, Qilu Hospital, Shandong University, 107 West Wenhua Rd, Jinan, 250012, People's Republic of China. daoxinma@sdu.edu.cn.
authors.forename: Daoxin
authors.initials: D
authors.lastname: Ma
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D053468:CARD Signaling Adaptor Proteins / Q000235:genetics; D005260:Female; D020022:Genetic Predisposition to Disease; D006801:Humans; D058847:Inflammasomes / Q000235:genetics*; D020382:Interleukin-18 / Q000097:blood / Q000235:genetics*; D053583:Interleukin-1beta / Q000097:blood / Q000235:genetics*; D015470:Leukemia, Myeloid, Acute / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D016328:NF-kappa B / Q000235:genetics; D000071199:NLR Family, Pyrin Domain-Containing 3 Protein / Q000235:genetics*; D009363:Neoplasm Proteins / Q000235:genetics; D020641:Polymorphism, Single Nucleotide; D011379:Prognosis; D055815:Young Adult
chemical_list: D053468:CARD Signaling Adaptor Proteins; C455623:CARD8 protein, human; C000589760:IL18 protein, human; D058847:Inflammasomes; D020382:Interleukin-18; D053583:Interleukin-1beta; D016328:NF-kappa B; D000071199:NLR Family, Pyrin Domain-Containing 3 Protein; C453881:NLRP3 protein, human; D009363:Neoplasm Proteins
keywords: Acute myeloid leukemia; Inflammasome, NLRP3; Polymorphism; Susceptibility