title: Aldosterone induces rapid sodium intake by a nongenomic mechanism in the nucleus tractus solitarius.
abstract: The purpose of this study was to determine whether aldosterone has a rapid action in the nucleus tractus solitarius (NTS) that increases sodium intake, and to examine whether this effect of aldosterone, if present, is mediated by G protein-coupled estrogen receptor (GPER). Adult male Sprague-Dawley rats with a stainless-steel cannula in the NTS were used. Aldosterone was injected into the NTS at the doses of 1, 5, 10 and 20 ng 0.1 μl-1. A rapid dose-related increase of 0.3 M NaCl intake was induced within 30 min and this increase was not suppressed by the mineralocorticoid receptor (MR) antagonist spironolactone (10 ng 0.1 μl-1). Water intake was not affected by aldosterone. The GPER agonist G-1 produced a parallel and significant increase in sodium intake, while pre-treatment with GPER antagonist G15 (10 ng 0.1 μl-1) blocked the G-1 or aldosterone-induced rapid sodium intake. In addition, sodium intake induced by sodium depletion or low-sodium diet fell within 30 min after injection into the NTS of the MR antagonist spironolactone, while G15 had no effect. Our results confirm previous reports, and support the hypothesis that aldosterone evokes rapid sodium intake through a non-genomic mechanism involving GPER in NTS.
authors.affiliation: Key Laboratory of Shaanxi Province for Craniofacial Precision Medicine Research, Xi'an Jiaotong University College of Stomatology, 98# Xiwu Road, Xi'an, Shaanxi 710000, P.R. China.|Department of Physiology and Pathophysiology, Xi'an Jiaotong University School of Basic Medical Science, 76# W. Yanta Road, Xi'an, Shaanxi 710061, P.R. China.|Department of Orthodontics, Xi'an Jiaotong University College of Stomatology, 98# Xiwu Road, Xi'an, Shaanxi 710000, P.R. China.
authors.forename: Hu
authors.initials: H
authors.lastname: Qiao
authors.affiliation: Department of Physiology and Pathophysiology, Xi'an Jiaotong University School of Basic Medical Science, 76# W. Yanta Road, Xi'an, Shaanxi 710061, P.R. China.
authors.forename: Bo
authors.initials: B
authors.lastname: Hu
authors.affiliation: Key Laboratory of Shaanxi Province for Craniofacial Precision Medicine Research, Xi'an Jiaotong University College of Stomatology, 98# Xiwu Road, Xi'an, Shaanxi 710000, P.R. China.|Department of Orthodontics, Xi'an Jiaotong University College of Stomatology, 98# Xiwu Road, Xi'an, Shaanxi 710000, P.R. China.
authors.forename: Hong
authors.initials: H
authors.lastname: Zhou
authors.affiliation: Key Laboratory of Shaanxi Province for Craniofacial Precision Medicine Research, Xi'an Jiaotong University College of Stomatology, 98# Xiwu Road, Xi'an, Shaanxi 710000, P.R. China.|Department of Physiology and Pathophysiology, Xi'an Jiaotong University School of Basic Medical Science, 76# W. Yanta Road, Xi'an, Shaanxi 710061, P.R. China.
authors.forename: Jianqun
authors.initials: J
authors.lastname: Yan
authors.affiliation: Department of Physiology and Pathophysiology, Xi'an Jiaotong University School of Basic Medical Science, 76# W. Yanta Road, Xi'an, Shaanxi 710061, P.R. China.
authors.forename: Ru
authors.initials: R
authors.lastname: Jia
authors.affiliation: Department of Physiology and Pathophysiology, Xi'an Jiaotong University School of Basic Medical Science, 76# W. Yanta Road, Xi'an, Shaanxi 710061, P.R. China.
authors.forename: Bo
authors.initials: B
authors.lastname: Lu
authors.affiliation: Department of Physiology and Pathophysiology, Xi'an Jiaotong University School of Basic Medical Science, 76# W. Yanta Road, Xi'an, Shaanxi 710061, P.R. China.
authors.forename: Bo
authors.initials: B
authors.lastname: Sun
authors.affiliation: Department of Physiology and Pathophysiology, Xi'an Jiaotong University School of Basic Medical Science, 76# W. Yanta Road, Xi'an, Shaanxi 710061, P.R. China.
authors.forename: Xiao
authors.initials: X
authors.lastname: Luo
authors.affiliation: Department of Physiology and Pathophysiology, Xi'an Jiaotong University School of Basic Medical Science, 76# W. Yanta Road, Xi'an, Shaanxi 710061, P.R. China.
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Fan
authors.affiliation: Department of Physiology and Pathophysiology, Xi'an Jiaotong University School of Basic Medical Science, 76# W. Yanta Road, Xi'an, Shaanxi 710061, P.R. China.
authors.forename: Nan
authors.initials: N
authors.lastname: Wang
mesh_terms: D000450:Aldosterone / Q000378:metabolism* / Q000494:pharmacology; D000818:Animals; D004305:Dose-Response Relationship, Drug; D008297:Male; D000451:Mineralocorticoid Receptor Antagonists / Q000494:pharmacology; D051381:Rats; D043562:Receptors, G-Protein-Coupled / Q000378:metabolism; D018161:Receptors, Mineralocorticoid / Q000378:metabolism; D012964:Sodium / Q000378:metabolism*; D012965:Sodium Chloride / Q000378:metabolism; D017552:Solitary Nucleus / Q000187:drug effects / Q000378:metabolism*; D013148:Spironolactone / Q000494:pharmacology
chemical_list: D000451:Mineralocorticoid Receptor Antagonists; D043562:Receptors, G-Protein-Coupled; D018161:Receptors, Mineralocorticoid; D013148:Spironolactone; D012965:Sodium Chloride; D000450:Aldosterone; D012964:Sodium