title: Population genomics diversity of Plasmodium falciparum in malaria patients attending Okelele Health Centre, Okelele, Ilorin, Kwara State, Nigeria.
abstract: BACKGROUND
  Plasmodium falciparum, the most dangerous malaria parasite species to humans remains an important public health concern in Okelele, a rural community in Ilorin, Kwara State, Nigeria. There is however little information about the genetic diversity ofPlasmodium falciparumin Nigeria.
  
  
  OBJECTIVE
  To determine the population genomic diversity ofPlasmodium falciparumin malaria patients attending Okelele Community Healthcare Centre, Okelele, Ilorin, Kwara State.
  
  
  METHODS
  In this study, 50Plasmodium falciparumstrains Merozoite Surface Protein 1, Merozoite Surface Protein 2 and Glutamate Rich Protein were analysed from Okelele Health Centre, Okelele, Ilorin, Nigeria. Genetic diversity ofP. falciparumisolates were analysed from nested polymerase chain reactions (PCR) of the MSP-1 (K1, MAD 20 and RO33), MSP-2 (FC27 and 3D7) and Glutamate Rich Protein allelic families respectively.
  
  
  RESULTS
  Polyclonal infections were more in majority of the patients for MSP-1 allelic families while monoclonal infections were more for MSP-2 allelic families. Multiplicity of infection for MSP-1, MSP-2 and GLURP were 1.7, 1.8 and 2.05 respectively.
  
  
  CONCLUSIONS
  There is high genetic diversity in MSP - 2 and GLURP allelic families ofPlasmodium falciparumisolates from Okelele Health Centre, Ilorin, Nigeria.
authors.affiliation: Infectious Diseases and Environmental Health Research Group, Department of Microbiology, University of Ilorin, Ilorin, Nigeria.
authors.forename: Olatunji Matthew
authors.initials: OM
authors.lastname: Kolawole
authors.affiliation: Paediatrics Department, University of Ilorin Teaching Hospital, Ilorin, Nigeria.
authors.forename: Olugbenga Ayodeji
authors.initials: OA
authors.lastname: Mokuolu
authors.affiliation: Department of Nutrition and Biochemistry, Nigerian Institute of Medical Research, Yaba, Lagos.
authors.forename: Yetunde Adeola
authors.initials: YA
authors.lastname: Olukosi
authors.affiliation: Infectious Diseases and Environmental Health Research Group, Department of Microbiology, University of Ilorin, Ilorin, Nigeria.
authors.forename: Tolulope Ololade
authors.initials: TO
authors.lastname: Oloyede
mesh_terms: D000483:Alleles; D014644:Genetic Variation; D005838:Genotype; D006801:Humans; D008288:Malaria; D056186:Metagenomics; D009549:Nigeria; D010963:Plasmodium falciparum / Q000235:genetics*; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA
keywords: Merozoite Surface Protein; Plasmodium falciparum; genetic diversity