title: Expression profiles and hormonal regulation of tobacco NtEXGT gene and its involvement in abiotic stress response.
abstract: Despite the intensive study of xyloglucan endotransglucosylases/hydrolases, their multifaceted role in plant growth regulation in changing environmental conditions is not yet clarified. The functional role of the large number of genes encoding this group of enzymes is also still unclear. NtEXGT gene encodes one of xyloglucan endotransglucosylases/hydrolases (XTHs) of Nicotiana tabacum L. The highest level of NtEXGT gene expression was detected in young flowers and leaves near the shoot apex. Expression of the NtEXGT gene in leaves was induced by cytokinins, auxins, brassinosteroids and gibberellins. NtEXGT gene was also up-regulated by salinity, drought, cold, cadmium and 10 μM abscisic acid treatments and down-regulated in response to 0 °C and 100 μM abscisic acid. Pretreatment of leaves with fluridone contributed to smaller increase in the level of NtEXGT transcripts in response to drought stress. These data suggest that NtEXGT gene is ABA-regulated and probably implicated in ABA-dependent signaling in response to stress factors. 35S::NtEXGT plants of tobacco showed higher rate of root growth under salt-stress conditions, greater frost and heat tolerance as compared with the wild type tobacco plants.
authors.affiliation: Institute of Biochemistry and Genetics, Ufa Scientific Centre, Russian Academy of Sciences (IBG USC RAS), pr. Oktyabrya 71, 450054, Ufa, Russia; Bashkir State University (BSU), Z. Validi str. 32, 450074, Ufa, Russia. Electronic address: kuluev@bk.ru.
authors.forename: Bulat
authors.initials: B
authors.lastname: Kuluev
authors.affiliation: Institute of Biochemistry and Genetics, Ufa Scientific Centre, Russian Academy of Sciences (IBG USC RAS), pr. Oktyabrya 71, 450054, Ufa, Russia; Bashkir State University (BSU), Z. Validi str. 32, 450074, Ufa, Russia.
authors.forename: Elena
authors.initials: E
authors.lastname: Mikhaylova
authors.affiliation: Institute of Biochemistry and Genetics, Ufa Scientific Centre, Russian Academy of Sciences (IBG USC RAS), pr. Oktyabrya 71, 450054, Ufa, Russia.
authors.forename: Zoya
authors.initials: Z
authors.lastname: Berezhneva
authors.affiliation: Institute of Biochemistry and Genetics, Ufa Scientific Centre, Russian Academy of Sciences (IBG USC RAS), pr. Oktyabrya 71, 450054, Ufa, Russia.
authors.forename: Yuri
authors.initials: Y
authors.lastname: Nikonorov
authors.affiliation: Institute of Biochemistry and Genetics, Ufa Scientific Centre, Russian Academy of Sciences (IBG USC RAS), pr. Oktyabrya 71, 450054, Ufa, Russia.
authors.forename: Bogdan
authors.initials: B
authors.lastname: Postrigan
authors.affiliation: Ufa Institute of Biology, Russian Academy of Sciences (UIB RAS), pr. Oktyabrya 69, 450054, Ufa, Russia.
authors.forename: Guzel
authors.initials: G
authors.lastname: Kudoyarova
authors.affiliation: Institute of Biochemistry and Genetics, Ufa Scientific Centre, Russian Academy of Sciences (IBG USC RAS), pr. Oktyabrya 71, 450054, Ufa, Russia.
authors.forename: Aleksey
authors.initials: A
authors.lastname: Chemeris
mesh_terms: D000040:Abscisic Acid / Q000494:pharmacology; D000085:Acetates / Q000494:pharmacology; D000222:Adaptation, Physiological / Q000187:drug effects / Q000235:genetics; D002104:Cadmium / Q000633:toxicity; D003080:Cold Temperature; D003517:Cyclopentanes / Q000494:pharmacology; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant / Q000187:drug effects*; D017343:Genes, Plant; D054883:Oxylipins / Q000494:pharmacology; D010802:Phylogeny; D010937:Plant Growth Regulators / Q000494:pharmacology*; D018515:Plant Leaves / Q000187:drug effects / Q000378:metabolism; D030821:Plants, Genetically Modified; D011728:Pyridones / Q000494:pharmacology; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D036226:Seedlings / Q000187:drug effects / Q000235:genetics; D012965:Sodium Chloride / Q000494:pharmacology; D013312:Stress, Physiological / Q000187:drug effects / Q000235:genetics*; D014026:Nicotiana / Q000187:drug effects / Q000235:genetics* / Q000502:physiology
chemical_list: D000085:Acetates; D003517:Cyclopentanes; D054883:Oxylipins; D010937:Plant Growth Regulators; D011728:Pyridones; D012333:RNA, Messenger; D002104:Cadmium; C013351:fluridone; D012965:Sodium Chloride; D000040:Abscisic Acid; C072239:methyl jasmonate
keywords: ARGOS-LIKE; Abscisic acid; Auxin; Cell expansion; Nicotiana tabacum L.; Stress tolerance; Xyloglucan endotransglucosylases/hydrolases