title: In vitro evaluation of electrospun silk fibroin/nano-hydroxyapatite/BMP-2 scaffolds for bone regeneration.
abstract: Bone tissue engineering by using osteoinductive scaffolds seeded with stem cells to promote bone extracellular matrix (ECM) production and remodeling has evolved into a promising approach for bone repair and regeneration. In order to mimic the ECM of bone tissue structurally and compositionally, nanofibrous silk fibroin (SF) scaffolds containing hydroxyapatite (HAP) nanoparticles and bone morphogenetic protein 2 (BMP-2) were fabricated in this study using electrospinning technique. The microstructure, mechanical property, biocompatibility, and osteogenic characteristics were examined. It was found that the HAP nanoparticles were successfully incorporated in the SF nanofibers (diameter, 200-500 nm). The mechanical properties of SF/HAP/BMP-2 composite scaffolds increased with HAP content when it was less than 20 wt%, after which the mechanical properties dropped as HAP content increased. Cell culture tests using bone marrow mesenchymal stem cells (BMSCs) showed that the scaffolds had good biocompatibility and promoted the osteogenic differentiation of BMSCs. Therefore, the electrospun SF/HAP/BMP-2 scaffolds may serve as a promising biomaterial for bone tissue engineering.
authors.affiliation: a Department of Orthopedics, Orthopaedic Institute, The First Affiliated Hospital , Soochow University , Suzhou , China.
authors.forename: Bingjie
authors.initials: B
authors.lastname: Niu
authors.affiliation: a Department of Orthopedics, Orthopaedic Institute, The First Affiliated Hospital , Soochow University , Suzhou , China.
authors.forename: Bin
authors.initials: B
authors.lastname: Li
authors.affiliation: a Department of Orthopedics, Orthopaedic Institute, The First Affiliated Hospital , Soochow University , Suzhou , China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Gu
authors.affiliation: a Department of Orthopedics, Orthopaedic Institute, The First Affiliated Hospital , Soochow University , Suzhou , China.
authors.forename: Xiaofeng
authors.initials: X
authors.lastname: Shen
authors.affiliation: a Department of Orthopedics, Orthopaedic Institute, The First Affiliated Hospital , Soochow University , Suzhou , China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Liu
authors.affiliation: a Department of Orthopedics, Orthopaedic Institute, The First Affiliated Hospital , Soochow University , Suzhou , China.
authors.forename: Liang
authors.initials: L
authors.lastname: Chen
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry / Q000494:pharmacology*; D055396:Bone Morphogenetic Protein 2 / Q000737:chemistry*; D001861:Bone Regeneration / Q000187:drug effects*; D002454:Cell Differentiation / Q000187:drug effects; D017886:Durapatite / Q000737:chemistry*; D004560:Electricity; D005349:Fibroins / Q000737:chemistry*; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000187:drug effects; D053758:Nanoparticles / Q000737:chemistry*; D036103:Nanotechnology; D010012:Osteogenesis / Q000187:drug effects; D051381:Rats
chemical_list: D001672:Biocompatible Materials; D055396:Bone Morphogenetic Protein 2; D005349:Fibroins; D017886:Durapatite
keywords: BMP-2; Silk fibroin; electrospun nanofibrous scaffolds; hydroxyapatite; osteogenic differentiation