title: Preadministration of Hydrogen-Rich Water Protects Against Lipopolysaccharide-Induced Sepsis and Attenuates Liver Injury.
abstract: Despite significant advances in antibiotic therapy and intensive care, sepsis remains the most common cause of death in intensive care units. We previously reported that molecular hydrogen (H2) acts as a therapeutic and preventive antioxidant. Here, we show that preadministration of H2-dissolved water (HW) suppresses lipopolysaccharide (LPS)-induced endotoxin shock in mice. Drinking HW for 3 days before LPS injection prolonged survival in a mouse model of sepsis. The H2 concentration immediately increased in the liver but not in the kidney after drinking HW. The protective effects of the preadministration of HW on LPS-induced liver injury were examined. Twenty-four hours after LPS injection, preadministration of HW reduced the increase in both apoptosis and oxidative stress. Moreover, preadministration of HW enhanced LPS-induced expression of heme oxyganase-1 and reduced endothelin-1 expression. These results indicate the therapeutic potential of HW in preventing acute injury of the liver with attenuation of an increase in oxidative stress. HW is likely to trigger adaptive responses against oxidative stress.
authors.affiliation: *Biological Process of Aging, Tokyo Metropolitan Institute of Gerontology, Tokyo, Japan †Faculty of Life Sciences, Toyo University, Gunma, Japan ‡Department of Pathology, Tokyo Metropolitan Institute of Gerontology, Tokyo, Japan.
authors.forename: Masumi
authors.initials: M
authors.lastname: Iketani
authors.forename: Jumi
authors.initials: J
authors.lastname: Ohshiro
authors.forename: Takuya
authors.initials: T
authors.lastname: Urushibara
authors.forename: Mayumi
authors.initials: M
authors.lastname: Takahashi
authors.forename: Tomio
authors.initials: T
authors.lastname: Arai
authors.forename: Hideo
authors.initials: H
authors.lastname: Kawaguchi
authors.forename: Ikuroh
authors.initials: I
authors.lastname: Ohsawa
mesh_terms: D000818:Animals; D056486:Chemical and Drug Induced Liver Injury / Q000097:blood / Q000188:drug therapy*; D004195:Disease Models, Animal; D006859:Hydrogen / Q000737:chemistry / Q000627:therapeutic use*; D020287:In Situ Nick-End Labeling; D015850:Interleukin-6 / Q000097:blood; D007668:Kidney / Q000187:drug effects / Q000293:injuries / Q000378:metabolism; D008070:Lipopolysaccharides / Q000633:toxicity*; D008099:Liver / Q000187:drug effects / Q000293:injuries / Q000378:metabolism; D008297:Male; D051379:Mice; D018384:Oxidative Stress / Q000187:drug effects; D018805:Sepsis / Q000097:blood / Q000139:chemically induced* / Q000188:drug therapy*; D014409:Tumor Necrosis Factor-alpha / Q000097:blood; D014867:Water / Q000737:chemistry / Q000494:pharmacology*
chemical_list: D015850:Interleukin-6; D008070:Lipopolysaccharides; D014409:Tumor Necrosis Factor-alpha; D014867:Water; D006859:Hydrogen