title: Calcium-mediated actin reset (CaAR) mediates acute cell adaptations.
abstract: Actin has well established functions in cellular morphogenesis. However, it is not well understood how the various actin assemblies in a cell are kept in a dynamic equilibrium, in particular when cells have to respond to acute signals. Here, we characterize a rapid and transient actin reset in response to increased intracellular calcium levels. Within seconds of calcium influx, the formin INF2 stimulates filament polymerization at the endoplasmic reticulum (ER), while cortical actin is disassembled. The reaction is then reversed within a few minutes. This Calcium-mediated actin reset (CaAR) occurs in a wide range of mammalian cell types and in response to many physiological cues. CaAR leads to transient immobilization of organelles, drives reorganization of actin during cell cortex repair, cell spreading and wound healing, and induces long-lasting changes in gene expression. Our findings suggest that CaAR acts as fundamental facilitator of cellular adaptations in response to acute signals and stress.
authors.affiliation: Institute of Cell Dynamics and Imaging, University of Muenster, Muenster, Germany.|Cells-In-Motion Cluster of Excellence (EXC1003 - CiM), University of Münster, Muenster, Germany.
authors.forename: Pauline
authors.initials: P
authors.lastname: Wales
authors.affiliation: Institute of Cell Dynamics and Imaging, University of Muenster, Muenster, Germany.|Cells-In-Motion Cluster of Excellence (EXC1003 - CiM), University of Münster, Muenster, Germany.
authors.forename: Christian E
authors.initials: CE
authors.lastname: Schuberth
authors.affiliation: Institute of Cell Dynamics and Imaging, University of Muenster, Muenster, Germany.|Cells-In-Motion Cluster of Excellence (EXC1003 - CiM), University of Münster, Muenster, Germany.
authors.forename: Roland
authors.initials: R
authors.lastname: Aufschnaiter
authors.affiliation: Institute of Cell Dynamics and Imaging, University of Muenster, Muenster, Germany.|Cells-In-Motion Cluster of Excellence (EXC1003 - CiM), University of Münster, Muenster, Germany.
authors.forename: Johannes
authors.initials: J
authors.lastname: Fels
authors.affiliation: Theory of Biological Matter, FOM Institute AMOLF, Amsterdam, Netherlands.
authors.forename: Ireth
authors.identifier: 0000-0003-2654-042X
authors.initials: I
authors.lastname: García-Aguilar
authors.affiliation: Institute of Cell Dynamics and Imaging, University of Muenster, Muenster, Germany.|Cells-In-Motion Cluster of Excellence (EXC1003 - CiM), University of Münster, Muenster, Germany.
authors.forename: Annette
authors.initials: A
authors.lastname: Janning
authors.affiliation: Institute of Cell Dynamics and Imaging, University of Muenster, Muenster, Germany.|Cells-In-Motion Cluster of Excellence (EXC1003 - CiM), University of Münster, Muenster, Germany.|Medical Clinic D, University Clinic of Muenster, Muenster, Germany.
authors.forename: Christopher P
authors.initials: CP
authors.lastname: Dlugos
authors.affiliation: Institute of Cell Dynamics and Imaging, University of Muenster, Muenster, Germany.|Cells-In-Motion Cluster of Excellence (EXC1003 - CiM), University of Münster, Muenster, Germany.
authors.forename: Marco
authors.initials: M
authors.lastname: Schäfer-Herte
authors.affiliation: Institute of Cell Dynamics and Imaging, University of Muenster, Muenster, Germany.|Cells-In-Motion Cluster of Excellence (EXC1003 - CiM), University of Münster, Muenster, Germany.|AG Molecular Mechanotransduction, Max Planck Institute of Biochemistry, Munich, Germany.
authors.forename: Christoph
authors.initials: C
authors.lastname: Klingner
authors.affiliation: Institute of Cell Dynamics and Imaging, University of Muenster, Muenster, Germany.|Cells-In-Motion Cluster of Excellence (EXC1003 - CiM), University of Münster, Muenster, Germany.
authors.forename: Mike
authors.initials: M
authors.lastname: Wälte
authors.affiliation: Institute of Cell Dynamics and Imaging, University of Muenster, Muenster, Germany.|Cells-In-Motion Cluster of Excellence (EXC1003 - CiM), University of Münster, Muenster, Germany.
authors.forename: Julian
authors.initials: J
authors.lastname: Kuhlmann
authors.affiliation: Institute of Cell Dynamics and Imaging, University of Muenster, Muenster, Germany.|Cells-In-Motion Cluster of Excellence (EXC1003 - CiM), University of Münster, Muenster, Germany.
authors.forename: Ekaterina
authors.initials: E
authors.lastname: Menis
authors.affiliation: Institute of Cell Dynamics and Imaging, University of Muenster, Muenster, Germany.|Cells-In-Motion Cluster of Excellence (EXC1003 - CiM), University of Münster, Muenster, Germany.
authors.forename: Laura
authors.initials: L
authors.lastname: Hockaday Kang
authors.affiliation: Department of Biochemistry, University of Munich, Munich, Germany.
authors.forename: Kerstin C
authors.initials: KC
authors.lastname: Maier
authors.affiliation: Institute of Biochemistry I, Friedrich Schiller University Jena, Jena, Germany.
authors.forename: Wenya
authors.initials: W
authors.lastname: Hou
authors.affiliation: Institute of Immunology, University of Münster, Münster, Germany.
authors.forename: Antonella
authors.initials: A
authors.lastname: Russo
authors.affiliation: Department of Biochemistry, Dartmouth Medical School, Hanover, United States.
authors.forename: Henry N
authors.initials: HN
authors.lastname: Higgs
authors.affiliation: Medical Clinic D, University Clinic of Muenster, Muenster, Germany.
authors.forename: Hermann
authors.initials: H
authors.lastname: Pavenstädt
authors.affiliation: Institute of Immunology, University of Münster, Münster, Germany.
authors.forename: Thomas
authors.initials: T
authors.lastname: Vogl
authors.affiliation: Institute of Immunology, University of Münster, Münster, Germany.
authors.forename: Johannes
authors.initials: J
authors.lastname: Roth
authors.affiliation: Institute of Biochemistry I, Friedrich Schiller University Jena, Jena, Germany.
authors.forename: Britta
authors.initials: B
authors.lastname: Qualmann
authors.affiliation: Institute of Biochemistry I, Friedrich Schiller University Jena, Jena, Germany.
authors.forename: Michael M
authors.initials: MM
authors.lastname: Kessels
authors.affiliation: Department of Biochemistry, University of Munich, Munich, Germany.
authors.forename: Dietmar E
authors.initials: DE
authors.lastname: Martin
authors.affiliation: Theory of Biological Matter, FOM Institute AMOLF, Amsterdam, Netherlands.
authors.forename: Bela
authors.initials: B
authors.lastname: Mulder
authors.affiliation: Institute of Cell Dynamics and Imaging, University of Muenster, Muenster, Germany.|Cells-In-Motion Cluster of Excellence (EXC1003 - CiM), University of Münster, Muenster, Germany.
authors.forename: Roland
authors.identifier: 0000-0002-1364-7589
authors.initials: R
authors.lastname: Wedlich-Söldner
mesh_terms: D008841:Actin Cytoskeleton / Q000378:metabolism; D000199:Actins / Q000378:metabolism*; D000222:Adaptation, Physiological; D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002460:Cell Line; D002468:Cell Physiological Phenomena; D006801:Humans
chemical_list: D000199:Actins; D002118:Calcium
keywords: actin cytoskeleton; calcium; cell biology; cell signaling; formins; human; mammalian cells; mouse