title: Proteomic Analysis of Dental Tissue Microsamples.
abstract: Improved understanding of dental enamel development will benefit not only dentistry but also biomedicine more generally. Rat and mouse models of enamel development are relatively well characterized and experimentally powerful. However, the diminutive size of murine teeth makes them difficult to study using standard proteomics approaches. Here, we describe gel-based proteomic methods that enable parallel quantification, identification, and functional characterization of proteins from developing rat and mouse teeth. These refined methods are applicable to other scarce samples including human enamel defects.
authors.affiliation: Department of Pharmacology and Therapeutics, University of Melbourne, Melbourne, VIC, Australia.
authors.forename: Jonathan E
authors.initials: JE
authors.lastname: Mangum
authors.affiliation: Department of Pharmacology and Therapeutics, University of Melbourne, Melbourne, VIC, Australia.|Department of Pediatrics, Royal Children's Hospital, University of Melbourne, Flemington Road, Melbourne, VIC, 3052, Australia.
authors.forename: Jew C
authors.initials: JC
authors.lastname: Kon
authors.affiliation: Department of Pharmacology and Therapeutics, University of Melbourne, Melbourne, VIC, Australia. mike.hubbard@unimelb.edu.au.|Department of Pediatrics, Royal Children's Hospital, University of Melbourne, Flemington Road, Melbourne, VIC, 3052, Australia. mike.hubbard@unimelb.edu.au.
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Hubbard
mesh_terms: D000818:Animals; D003743:Dental Enamel / Q000378:metabolism*; D015180:Electrophoresis, Gel, Two-Dimensional; D004848:Epithelium / Q000378:metabolism; D005109:Extracellular Matrix / Q000378:metabolism; D006801:Humans; D013058:Mass Spectrometry; D051379:Mice; D020543:Proteome; D040901:Proteomics / Q000379:methods; D051381:Rats
chemical_list: D020543:Proteome
keywords: Ameloblast; Dental development; Enamel defects; Functional proteomics; Gel electrophoresis; Microsample proteomics; Rat and mouse models; Sample preparation