title: Fluoride affects bone repair differently in mice models with distinct bone densities.
abstract: We grouped mice [strains: C57BL/6J (n=32) and C3H/HeJ (n=32)] to address the influence of bone density on fluoride's (F's) biological effects. These animals received low-fluoride food and water containing 0 (control group) or 50ppm of F for up to 28days. The upper left central incisor was extracted, and the left maxilla was collected at 7, 14, 21, and 28days for histological and histomorphometric analysis to estimate bone neoformation. Our results showed bone neoformation in all of the evaluated groups, with the presence of bone islets invading the center of the alveoli when replacing the existing connective tissue. Curiously, this biological phenomenon was more evident in the C57BL/6J strain. The histomorphometric analysis confirmed the histological findings in relation to the amount of new bone tissue and showed a decrease in C3H/HeJ mice (control group). Altogether, our results showed differential effects of fluoride bone metabolism, confirming a genetic component in susceptibility to the effects of fluoride.
authors.affiliation: Department of Biological Sciences, Bauru School of Dentistry, University of São Paulo, Al. Octávio Pinheiro Brisolla, 9-75, 17012-901, Bauru, São Paulo, Brazil.
authors.forename: Paula Kennerly
authors.initials: PK
authors.lastname: Herrera
authors.affiliation: Department of Biological Sciences, Bauru School of Dentistry, University of São Paulo, Al. Octávio Pinheiro Brisolla, 9-75, 17012-901, Bauru, São Paulo, Brazil.
authors.forename: Andressa Pelissari
authors.initials: AP
authors.lastname: Zambolin
authors.affiliation: Department of Genetics and Evolution, Center of Biological Sciences and Health, Federal University of São Carlos, Washington Luis, km 235, 13560-970, São Carlos, São Paulo, Brazil.
authors.forename: Mileni da Silva
authors.initials: MDS
authors.lastname: Fernandes
authors.affiliation: Department of Biological Sciences, Bauru School of Dentistry, University of São Paulo, Al. Octávio Pinheiro Brisolla, 9-75, 17012-901, Bauru, São Paulo, Brazil.
authors.forename: Tânia Mary
authors.initials: TM
authors.lastname: Cestari
authors.affiliation: Department of Biological Sciences, Bauru School of Dentistry, University of São Paulo, Al. Octávio Pinheiro Brisolla, 9-75, 17012-901, Bauru, São Paulo, Brazil.
authors.forename: Flávia Godoy
authors.initials: FG
authors.lastname: Iano
authors.affiliation: Laboratory for Bioassays and Cellular Dynamics, Department of Chemistry and Biochemistry, Bioscience Institute, São Paulo State University, Rubião Junior S/N, 18618-970, Botucatu, São Paulo, Brazil.
authors.forename: Willian Fernando
authors.initials: WF
authors.lastname: Zambuzzi
authors.affiliation: Department of Biological Sciences, Bauru School of Dentistry, University of São Paulo, Al. Octávio Pinheiro Brisolla, 9-75, 17012-901, Bauru, São Paulo, Brazil.
authors.forename: Marília Afonso Rabelo
authors.initials: MAR
authors.lastname: Buzalaf
authors.affiliation: Department of Biological Sciences, Bauru School of Dentistry, University of São Paulo, Al. Octávio Pinheiro Brisolla, 9-75, 17012-901, Bauru, São Paulo, Brazil. Electronic address: rodrigocardoso@usp.br.
authors.forename: Rodrigo Cardoso de
authors.initials: RC
authors.lastname: Oliveira
mesh_terms: D000818:Animals; D015519:Bone Density / Q000187:drug effects / Q000502:physiology*; D016723:Bone Remodeling / Q000187:drug effects*; D001842:Bone and Bones / Q000187:drug effects* / Q000502:physiology; D005459:Fluorides / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology*; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL
chemical_list: D005459:Fluorides
keywords: Bone repair; Bone tissue; Fluoride; Mouse