title: Evaluation of 19,460 Wheat Accessions Conserved in the Indian National Genebank to Identify New Sources of Resistance to Rust and Spot Blotch Diseases.
abstract: A comprehensive germplasm evaluation study of wheat accessions conserved in the Indian National Genebank was conducted to identify sources of rust and spot blotch resistance. Genebank accessions comprising three species of wheat-Triticum aestivum, T. durum and T. dicoccum were screened sequentially at multiple disease hotspots, during the 2011-14 crop seasons, carrying only resistant accessions to the next step of evaluation. Wheat accessions which were found to be resistant in the field were then assayed for seedling resistance and profiled using molecular markers. In the primary evaluation, 19,460 accessions were screened at Wellington (Tamil Nadu), a hotspot for wheat rusts. We identified 4925 accessions to be resistant and these were further evaluated at Gurdaspur (Punjab), a hotspot for stripe rust and at Cooch Behar (West Bengal), a hotspot for spot blotch. The second round evaluation identified 498 accessions potentially resistant to multiple rusts and 868 accessions potentially resistant to spot blotch. Evaluation of rust resistant accessions for seedling resistance against seven virulent pathotypes of three rusts under artificial epiphytotic conditions identified 137 accessions potentially resistant to multiple rusts. Molecular analysis to identify different combinations of genetic loci imparting resistance to leaf rust, stem rust, stripe rust and spot blotch using linked molecular markers, identified 45 wheat accessions containing known resistance genes against all three rusts as well as a QTL for spot blotch resistance. The resistant germplasm accessions, particularly against stripe rust, identified in this study can be excellent potential candidates to be employed for breeding resistance into the background of high yielding wheat cultivars through conventional or molecular breeding approaches, and are expected to contribute toward food security at national and global levels.
authors.affiliation: ICAR-National Bureau of Plant Genetic Resources, Pusa Campus, New Delhi, India.
authors.forename: Sundeep
authors.initials: S
authors.lastname: Kumar
authors.affiliation: ICAR-National Bureau of Plant Genetic Resources, Pusa Campus, New Delhi, India.
authors.forename: Sunil
authors.identifier: http://orcid.org/0000-0002-9146-2993
authors.initials: S
authors.lastname: Archak
authors.affiliation: ICAR-National Bureau of Plant Genetic Resources, Pusa Campus, New Delhi, India.
authors.forename: R K
authors.initials: RK
authors.lastname: Tyagi
authors.affiliation: ICAR-Indian Agricultural Research Institute, Regional Station, Wellington, Tamil Nadu, India.
authors.forename: Jagdish
authors.initials: J
authors.lastname: Kumar
authors.affiliation: ICAR-Indian Agricultural Research Institute, Regional Station, Wellington, Tamil Nadu, India.
authors.forename: Vikas
authors.initials: V
authors.lastname: Vk
authors.affiliation: ICAR-National Bureau of Plant Genetic Resources, Pusa Campus, New Delhi, India.
authors.forename: Sherry R
authors.initials: SR
authors.lastname: Jacob
authors.affiliation: ICAR-National Bureau of Plant Genetic Resources, Pusa Campus, New Delhi, India.
authors.forename: Kalyani
authors.initials: K
authors.lastname: Srinivasan
authors.affiliation: ICAR-National Bureau of Plant Genetic Resources, Pusa Campus, New Delhi, India.
authors.forename: J
authors.initials: J
authors.lastname: Radhamani
authors.affiliation: ICAR-National Bureau of Plant Genetic Resources, Pusa Campus, New Delhi, India.
authors.forename: R
authors.initials: R
authors.lastname: Parimalan
authors.affiliation: ICAR-Indian Agricultural Research Institute, Regional Station, Wellington, Tamil Nadu, India.
authors.forename: M
authors.initials: M
authors.lastname: Sivaswamy
authors.affiliation: ICAR-National Bureau of Plant Genetic Resources, Pusa Campus, New Delhi, India.
authors.forename: Sandhya
authors.initials: S
authors.lastname: Tyagi
authors.affiliation: ICAR-National Bureau of Plant Genetic Resources, Pusa Campus, New Delhi, India.
authors.forename: Mamata
authors.initials: M
authors.lastname: Yadav
authors.affiliation: ICAR-National Bureau of Plant Genetic Resources, Pusa Campus, New Delhi, India.
authors.forename: Jyotisna
authors.initials: J
authors.lastname: Kumari
authors.affiliation: ICAR-National Bureau of Plant Genetic Resources, Pusa Campus, New Delhi, India.
authors.lastname: Deepali
authors.affiliation: ICAR-National Bureau of Plant Genetic Resources, Pusa Campus, New Delhi, India.
authors.forename: Sandeep
authors.initials: S
authors.lastname: Sharma
authors.affiliation: Punjab Agricultural University, Regional Station, Gurdaspur, Punjab, India.
authors.forename: Indoo
authors.initials: I
authors.lastname: Bhagat
authors.affiliation: Punjab Agricultural University, Ludhiana, Punjab, India.
authors.forename: Madhu
authors.initials: M
authors.lastname: Meeta
authors.affiliation: Punjab Agricultural University, Ludhiana, Punjab, India.
authors.forename: N S
authors.initials: NS
authors.lastname: Bains
authors.affiliation: North Bengal Agricultural University, Cooch Behar, West Bengal, India.
authors.forename: A K
authors.initials: AK
authors.lastname: Chowdhury
authors.affiliation: North Bengal Agricultural University, Cooch Behar, West Bengal, India.
authors.forename: B C
authors.initials: BC
authors.lastname: Saha
authors.affiliation: North Bengal Agricultural University, Cooch Behar, West Bengal, India.
authors.forename: P M
authors.initials: PM
authors.lastname: Bhattacharya
authors.affiliation: ICAR-National Bureau of Plant Genetic Resources, Pusa Campus, New Delhi, India.
authors.forename: Jyoti
authors.initials: J
authors.lastname: Kumari
authors.affiliation: ICAR-National Bureau of Plant Genetic Resources, Pusa Campus, New Delhi, India.
authors.forename: M C
authors.initials: MC
authors.lastname: Singh
authors.affiliation: ICAR-Indian Institute of Wheat and Barley Research, Regional Station, Flowerdale, Himachal Pradesh, India.
authors.forename: O P
authors.initials: OP
authors.lastname: Gangwar
authors.affiliation: ICAR-Indian Institute of Wheat and Barley Research, Regional Station, Flowerdale, Himachal Pradesh, India.
authors.forename: P
authors.initials: P
authors.lastname: Prasad
authors.affiliation: ICAR-Indian Institute of Wheat and Barley Research, Regional Station, Flowerdale, Himachal Pradesh, India.
authors.forename: S C
authors.initials: SC
authors.lastname: Bharadwaj
authors.affiliation: ICAR-Indian Agricultural Research Institute, New Delhi, India.
authors.forename: Robin
authors.initials: R
authors.lastname: Gogoi
authors.affiliation: ICAR-Indian Agricultural Research Institute, New Delhi, India.
authors.forename: J B
authors.initials: JB
authors.lastname: Sharma
authors.affiliation: ICAR-Indian Agricultural Research Institute, Regional Station, Katrain, Himachal Pradesh, India.
authors.forename: Sandeep Kumar
authors.initials: SK
authors.lastname: Gm
authors.affiliation: ICAR-Indian Institute of Wheat and Barley Research, Karnal, Haryana, India.
authors.forename: M S
authors.initials: MS
authors.lastname: Saharan
authors.affiliation: ICAR-National Bureau of Plant Genetic Resources, Pusa Campus, New Delhi, India.
authors.forename: Manas
authors.initials: M
authors.lastname: Bag
authors.affiliation: ICAR-National Bureau of Plant Genetic Resources, Pusa Campus, New Delhi, India.
authors.forename: Anirban
authors.initials: A
authors.lastname: Roy
authors.affiliation: ICAR-National Bureau of Plant Genetic Resources, Pusa Campus, New Delhi, India.
authors.forename: T V
authors.initials: TV
authors.lastname: Prasad
authors.affiliation: ICAR-National Bureau of Plant Genetic Resources, Pusa Campus, New Delhi, India.
authors.forename: R K
authors.initials: RK
authors.lastname: Sharma
authors.affiliation: ICAR-National Bureau of Plant Genetic Resources, Pusa Campus, New Delhi, India.
authors.forename: M
authors.initials: M
authors.lastname: Dutta
authors.affiliation: ICAR-Indian Institute of Wheat and Barley Research, Karnal, Haryana, India.
authors.forename: Indu
authors.initials: I
authors.lastname: Sharma
authors.affiliation: ICAR-National Bureau of Plant Genetic Resources, Pusa Campus, New Delhi, India.
authors.forename: K C
authors.initials: KC
authors.lastname: Bansal
mesh_terms: D001203:Ascomycota / Q000472:pathogenicity; D030541:Databases, Genetic; D060467:Disease Resistance; D007194:India; D040641:Quantitative Trait Loci; D014908:Triticum / Q000145:classification / Q000235:genetics* / Q000276:immunology / Q000382:microbiology