title: Chromosome Preparation for Myeloid Malignancies.
abstract: Many cases of myeloid malignancies are associated with recurring cytogenetic aberrations. Chromosomal analysis can aid in diagnosis, predict prognosis, and disclose subsequent clonal evolution. Three different cell culture methods: direct harvest, nonsynchronized culture, and synchronized culture are usually prepared if the nucleated cell counts in marrow blood are sufficient. Synchronized culture is the first choice of method in myeloid malignancies, whereas the direct method can be omitted if the cell count is low. The aseptic culture technique is strictly followed until harvesting procedure. For synchronized culture, uridine and fluorodeoxyuridine are added as blocking reagents and released by thymidine on the following day. Harvesting steps of the cultures involved colcemid exposure, hypotonic treatment, and Carnoy's fixation. The cells are then ready for slide making and banding for chromosomal analysis.
authors.affiliation: Haematology Division, Department of Anatomical and Cellular Pathology, Prince of Wales Hospital, Shatin, Hong Kong.
authors.forename: Eleanor K C
authors.initials: EK
authors.lastname: Hui
authors.affiliation: Haematology Division, Department of Anatomical and Cellular Pathology, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, Hong Kong.
authors.forename: Thomas S K
authors.initials: TS
authors.lastname: Wan
authors.affiliation: Haematology Division, Department of Anatomical and Cellular Pathology, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, Hong Kong. margaretng@cuhk.edu.hk.
authors.forename: Margaret H L
authors.initials: MH
authors.lastname: Ng
mesh_terms: D001853:Bone Marrow / Q000473:pathology; D001854:Bone Marrow Cells / Q000378:metabolism / Q000473:pathology; D002452:Cell Count; D002478:Cells, Cultured; D002869:Chromosome Aberrations; D020732:Cytogenetic Analysis / Q000379:methods; D006801:Humans; D009196:Myeloproliferative Disorders / Q000175:diagnosis* / Q000235:genetics*
keywords: Chromosome preparation; Cytogenetic culture; Metaphase harvesting; Myeloid malignancies; Synchronization culture