title: Deep eutectic solvents as green media for extraction of flavonoid glycosides and aglycones from Platycladi Cacumen.
abstract: Deep eutectic solvents (DESs) are emerging as alternatives to conventional ionic liquids and organic solvents due to their unique advantages. In the present study, the tuneability of DESs as tailor-made solvents to efficiently extract polar and non-polar bioactive compounds from Platycladi Cacumen was detailedly investigated. Totally 12 types of choline chloride-, betaine-, and l-proline-based DESs were synthesized for initial screening, and extraction conditions was optimized by single-factor experiment. Experiments with different DESs and principal components analysis demonstrated that the extractability of both flavonoid glycosides and aglycones was greater with certain designed DESs than conventional solvents. In addition, the water content in DESs led to significantly different extraction yields of flavonoid compounds. The target compounds were recovered from DESs by macroporous resin LX-38 with a satisfactory yield between 77.44% and 98.92%. The knowledge acquired in this study could contribute to further DES application in extraction of bioactive compounds from natural sources.
authors.affiliation: State Key Laboratory of Natural Medicines, China Pharmaceutical University, No. 24 Tongjia Lane, Nanjing, PR China.
authors.forename: Bo
authors.initials: B
authors.lastname: Zhuang
authors.affiliation: State Key Laboratory of Natural Medicines, China Pharmaceutical University, No. 24 Tongjia Lane, Nanjing, PR China.
authors.forename: Li-Li
authors.initials: LL
authors.lastname: Dou
authors.affiliation: State Key Laboratory of Natural Medicines, China Pharmaceutical University, No. 24 Tongjia Lane, Nanjing, PR China.
authors.forename: Ping
authors.initials: P
authors.lastname: Li
authors.affiliation: State Key Laboratory of Natural Medicines, China Pharmaceutical University, No. 24 Tongjia Lane, Nanjing, PR China. Electronic address: liuehu2011@163.com.
authors.forename: E-Hu
authors.initials: EH
authors.lastname: Liu
mesh_terms: D004365:Drugs, Chinese Herbal / Q000032:analysis*; D005419:Flavonoids / Q000032:analysis*; D006027:Glycosides / Q000032:analysis*; D055772:Green Chemistry Technology / Q000379:methods*; D039823:Iridoids / Q000032:analysis*; D012997:Solvents / Q000737:chemistry
chemical_list: D004365:Drugs, Chinese Herbal; D005419:Flavonoids; D006027:Glycosides; D039823:Iridoids; D012997:Solvents
keywords: Biflavones aglycones; Deep eutectic solvents; Extraction; Flavonoid glycosides; Macroporous resin; Platycladi cacumen