title: MiR-211 inhibits invasion and epithelial-to-mesenchymal transition (EMT) of cervical cancer cells via targeting MUC4.
abstract: The dysregulated molecules and their involvement in lymph node metastases of cervical cancer are far from been fully revealed. In this study, by reviewing MUC4 expression in The Human Protein Atlas and retrieving gene microarray data in GEO dataset (No. GDS4664), we found that MUC4 upregulation is associated with lymph node metastasis in cervical cancer. Knockdown of MUC4 in Hela and SiHa cells significantly reduced their invasion and also reduced the mesenchymal properties. By performing bioinformatics analysis, we observed that miR-211 is a potential suppressor of MUC4, which has a predicted highly conserved binding site in the 3'UTR of MUC among mammals. The following assays confirmed that miR-211 can directly target the 3'UTR of MUC4 and inhibit its expression at both mRNA and protein levels. In addition, enforced miR-211 expression phenocopies the effects of MUC4 siRNA in inhibiting cervical cancer cell invasion and reversing EMT properties. Therefore, we infer that miR-211 is a novel miRNA with suppressive effect on MUC4 expression and can inhibit cervical cancer cell invasion and EMT.
authors.affiliation: Department of Obstetrics & Gynaecology, Cangzhou Central Hospital, Hebei, 061001, China.
authors.forename: Dongkui
authors.initials: D
authors.lastname: Xu
authors.affiliation: Department of Obstetrics & Gynaecology, Cangzhou Central Hospital, Hebei, 061001, China.
authors.forename: Shikai
authors.initials: S
authors.lastname: Liu
authors.affiliation: Department of Obstetrics & Gynaecology, Cangzhou Central Hospital, Hebei, 061001, China.
authors.forename: Liang
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Department of Obstetrics & Gynaecology, Cangzhou Central Hospital, Hebei, 061001, China. Electronic address: commasll@163.com.
authors.forename: Lili
authors.initials: L
authors.lastname: Song
mesh_terms: D020413:3' Untranslated Regions / Q000235:genetics; D001483:Base Sequence; D015153:Blotting, Western; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D058750:Epithelial-Mesenchymal Transition / Q000235:genetics*; D005260:Female; D020869:Gene Expression Profiling / Q000706:statistics & numerical data; D015972:Gene Expression Regulation, Neoplastic; D006367:HeLa Cells; D006801:Humans; D035683:MicroRNAs / Q000235:genetics*; D008856:Microscopy, Fluorescence; D055264:Mucin-4 / Q000235:genetics* / Q000378:metabolism; D009361:Neoplasm Invasiveness; D020411:Oligonucleotide Array Sequence Analysis / Q000706:statistics & numerical data; D034622:RNA Interference; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012689:Sequence Homology, Nucleic Acid; D002583:Uterine Cervical Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology
chemical_list: D020413:3' Untranslated Regions; C549405:MIRN211 microRNA, human; C524658:MUC4 protein, human; D035683:MicroRNAs; D055264:Mucin-4
keywords: Cervical cancer; Epithelial-to-mesenchymal transition; MUC4; MiR-211