title: Preparation, Physicochemical Characterization and Anti-fungal Evaluation of Nystatin-Loaded PLGA-Glucosamine Nanoparticles.
abstract: Nystatin loaded PLGA and PLGA-Glucosamine nanoparticles were formulated. PLGA were functionalized with Glucosamine (PLGA-GlcN) to enhance the adhesion of nanoparticles to Candida Albicans (C.albicans) cell walls.
  
  
  
  Quasi-emulsion solvent diffusion method was employed using PLGA and PLGA-GlcN with various drug-polymer ratios for the preparation of nanoparticles. The nanoparticles were evaluated for size, zeta potential, polydispersity index, drug crystallinity, loading efficiency and release properties. DSC, SEM, XRPD,1H-NMR, and FT-IR were performed to analyze the physicochemical properties of the nanoparticles. Antifungal activity of the nanoparticles was evaluated by determination of MICs against C.albicans.
  
  
  
  The spectra of1H-NMR and FT-IR analysis ensured GlcN functionalization on PLGA nanoparticles. SEM characterization confirmed that particles were in the nanosize range and the particle size for PLGA and PLGA-GlcN nanoparticles were in the range of 108.63 ± 4.5 to 168.8 ± 5.65 nm and 208.76 ± 16.85 nm, respectively. DSC and XRPD analysis ensured reduction of the drug crystallinity in the nanoparticles. PLGA-GlcN nanoparticles exhibit higher antifungal activity than PLGA nanoparticles.
  
  
  
  PLGA-GlcN nanoparticles showed more antifungal activity with appropriate physicochemical properties than pure Nystatin and PLGA nanoparticles.
authors.affiliation: Novel Drug Delivery Research Center, Faculty of Pharmacy, Kermanshah University of Medical Sciences,, Kermanshah, Iran. ghobadmohammadi@yahoo.com.|Faculty of Pharmacy, Kermanshah University of Medical Sciences, Kermanshah, Iran. ghobadmohammadi@yahoo.com.
authors.forename: Ghobad
authors.initials: G
authors.lastname: Mohammadi
authors.affiliation: Student Research Committee, Kermanshah University of Medical Sciences,, Kermanshah, Iran.
authors.forename: Amineh
authors.initials: A
authors.lastname: Shakeri
authors.affiliation: Novel Drug Delivery Research Center, Faculty of Pharmacy, Kermanshah University of Medical Sciences,, Kermanshah, Iran.
authors.forename: Ali
authors.initials: A
authors.lastname: Fattahi
authors.affiliation: Novel Drug Delivery Research Center, Faculty of Pharmacy, Kermanshah University of Medical Sciences,, Kermanshah, Iran.
authors.forename: Pardis
authors.initials: P
authors.lastname: Mohammadi
authors.affiliation: Faculty of Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran.
authors.forename: Ali
authors.initials: A
authors.lastname: Mikaeili
authors.affiliation: Department of Medicinal Chemistry, Faculty of Pharmacy, Kermanshah University of Medical Sciences,, Kermanshah, Iran.
authors.forename: Alireza
authors.initials: A
authors.lastname: Aliabadi
authors.affiliation: Faculty of Pharmacy, Tabriz University of Medical Sciences,, Tabriz, Iran.
authors.forename: Khosro
authors.initials: K
authors.lastname: Adibkia
mesh_terms: D000935:Antifungal Agents / Q000737:chemistry* / Q000494:pharmacology; D002176:Candida albicans / Q000187:drug effects; D002626:Chemistry, Pharmaceutical / Q000379:methods; D004337:Drug Carriers / Q000737:chemistry; D005944:Glucosamine / Q000737:chemistry*; D019344:Lactic Acid / Q000737:chemistry*; D053758:Nanoparticles / Q000737:chemistry*; D009761:Nystatin / Q000737:chemistry* / Q000494:pharmacology; D010316:Particle Size; D011100:Polyglycolic Acid / Q000737:chemistry*; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods
chemical_list: D000935:Antifungal Agents; D004337:Drug Carriers; D009761:Nystatin; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; D005944:Glucosamine
keywords: Candida albicans; PLGA; glucosamine; nanoparticles; nystatin