title: Redox and pH Dual Responsive Polymer Based Nanoparticles for In Vivo Drug Delivery.
abstract: Responsive nanomaterials have emerged as promising candidates as drug delivery vehicles in order to address biomedical diseases such as cancer. In this work, polymer-based responsive nanoparticles prepared by a supramolecular approach are loaded with doxorubicin (DOX) for the cancer therapy. The nanoparticles contain disulfide bonds within the polymer network, allowing the release of the DOX payload in a reducing environment within the endoplasm of cancer cells. In addition, the loaded drug can also be released under acidic environment. In vitro anticancer studies using redox and pH dual responsive nanoparticles show excellent performance in inducing cell death and apoptosis. Zebrafish larvae treated with DOX-loaded nanoparticles exhibit an improved viability as compared with the cases treated with free DOX by the end of a 3 d treatment. Confocal imaging is utilized to provide the daily assessment of tumor size on zebrafish larva models treated with DOX-loaded nanoparticles, presenting sustainable reduction of tumor. This work demonstrates the development of functional nanoparticles with dual responsive properties for both in vitro and in vivo drug delivery in the cancer therapy.
authors.affiliation: Division of Chemistry and Biological Chemistry, School of Physical and Mathematical Sciences, Nanyang Technological University, 21 Nanyang Link, Singapore, 637371, Singapore.|Department of Chemical Engineering, School of Applied Science, Temasek Polytechnic, 21 Tampines Ave 1, Singapore, 529757, Singapore.
authors.forename: Chung Yen
authors.initials: CY
authors.lastname: Ang
authors.affiliation: Division of Chemistry and Biological Chemistry, School of Physical and Mathematical Sciences, Nanyang Technological University, 21 Nanyang Link, Singapore, 637371, Singapore.
authors.forename: Si Yu
authors.initials: SY
authors.lastname: Tan
authors.affiliation: Institute of Molecular Cell Biology, 61 Biopolis Drive, Singapore, 138673, Singapore.
authors.forename: Cathleen
authors.initials: C
authors.lastname: Teh
authors.affiliation: Division of Chemistry and Biological Chemistry, School of Physical and Mathematical Sciences, Nanyang Technological University, 21 Nanyang Link, Singapore, 637371, Singapore.
authors.forename: Jia Min
authors.initials: JM
authors.lastname: Lee
authors.affiliation: Division of Chemistry and Biological Chemistry, School of Physical and Mathematical Sciences, Nanyang Technological University, 21 Nanyang Link, Singapore, 637371, Singapore.
authors.forename: Mun Fei Eddy
authors.initials: MF
authors.lastname: Wong
authors.affiliation: Division of Chemistry and Biological Chemistry, School of Physical and Mathematical Sciences, Nanyang Technological University, 21 Nanyang Link, Singapore, 637371, Singapore.
authors.forename: Qiuyu
authors.initials: Q
authors.lastname: Qu
authors.affiliation: Division of Chemistry and Biological Chemistry, School of Physical and Mathematical Sciences, Nanyang Technological University, 21 Nanyang Link, Singapore, 637371, Singapore.
authors.forename: Li Qing
authors.initials: LQ
authors.lastname: Poh
authors.affiliation: School of Materials Science and Engineering, Nanyang Technological University, 60 Nanyang Drive, Singapore, 637551, Singapore.
authors.forename: Menghuan
authors.initials: M
authors.lastname: Li
authors.affiliation: Division of Chemistry and Biological Chemistry, School of Physical and Mathematical Sciences, Nanyang Technological University, 21 Nanyang Link, Singapore, 637371, Singapore.
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Institute of Molecular Cell Biology, 61 Biopolis Drive, Singapore, 138673, Singapore.
authors.forename: Vladimir
authors.initials: V
authors.lastname: Korzh
authors.affiliation: Division of Chemistry and Biological Chemistry, School of Physical and Mathematical Sciences, Nanyang Technological University, 21 Nanyang Link, Singapore, 637371, Singapore.|School of Materials Science and Engineering, Nanyang Technological University, 60 Nanyang Drive, Singapore, 637551, Singapore.
authors.forename: Yanli
authors.initials: Y
authors.lastname: Zhao
mesh_terms: D000143:Acids / Q000737:chemistry; D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology; D004317:Doxorubicin / Q000494:pharmacology / Q000627:therapeutic use; D016503:Drug Delivery Systems; D004705:Endocytosis / Q000187:drug effects; D006367:HeLa Cells; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007814:Larva / Q000187:drug effects; D008113:Liver Neoplasms / Q000188:drug therapy / Q000473:pathology; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D010084:Oxidation-Reduction; D011108:Polymers / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared; D015027:Zebrafish / Q000378:metabolism
chemical_list: D000143:Acids; D000970:Antineoplastic Agents; D011108:Polymers; D004317:Doxorubicin
keywords: polymeric nanoparticles; responsive release; self-assembly; tumor; zebrafish, drug delivery