title: Does Antibiotic Resistance Evolve in Hospitals?
abstract: Nosocomial outbreaks of bacteria are well documented. Based on these incidents, and the heavy usage of antibiotics in hospitals, it has been assumed that antibiotic resistance evolves in hospital environments. To test this assumption, we studied resistance phenotypes of bacteria collected from patient isolates at a community hospital over a 2.5-year period. A graphical model analysis shows no association between resistance and patient information other than time of arrival. This allows us to focus on time-course data. We introduce a hospital transmission model, based on negative binomial delay. Our main contribution is a statistical hypothesis test called the Nosocomial Evolution of Resistance Detector (NERD). It calculates the significance of resistance trends occurring in a hospital. It can inform hospital staff about the effects of various practices and interventions, can help detect clonal outbreaks, and is available as an R package. We applied the NERD method to each of the 16 antibiotics in the study via 16 hypothesis tests. For 13 of the antibiotics, we found that the hospital environment had no significant effect on the evolution of resistance; the hospital is merely a piece of the larger picture. The p-values obtained for the other three antibiotics (cefepime, ceftazidime, and gentamicin) indicate that particular care should be taken in hospital practices with these antibiotics. One of the three, ceftazidime, was significant after accounting for multiple hypotheses, indicating a trend of decreased resistance for this drug.
authors.affiliation: Department of Mathematics, University of California, Berkeley, Berkeley, CA, USA. seigal@berkeley.edu.
authors.forename: Anna
authors.initials: A
authors.lastname: Seigal
authors.affiliation: School of Natural Sciences, University of California, Merced, Merced, CA, USA.
authors.forename: Portia
authors.initials: P
authors.lastname: Mira
authors.affiliation: Department of Mathematics, University of California, Berkeley, Berkeley, CA, USA.
authors.forename: Bernd
authors.initials: B
authors.lastname: Sturmfels
authors.affiliation: School of Natural Sciences, University of California, Merced, Merced, CA, USA.
authors.forename: Miriam
authors.initials: M
authors.lastname: Barlow
mesh_terms: D001424:Bacterial Infections / Q000188:drug therapy* / Q000453:epidemiology / Q000382:microbiology*; D003428:Cross Infection / Q000188:drug therapy* / Q000453:epidemiology / Q000382:microbiology*; D004196:Disease Outbreaks; D024881:Drug Resistance, Bacterial / Q000235:genetics*; D019143:Evolution, Molecular; D006801:Humans; D055641:Mathematical Concepts; D008954:Models, Biological
keywords: Antibiotic resistance; Infection control; Negative binomial distribution; Nosocomial outbreaks