title: Clinical phenotype of recurrent aphthous stomatitis and interleukin-1β genotype in a Polish cohort of patients.
abstract: BACKGROUND
  The aim of this study was to evaluate the relation between a clinical profile and the interleukin-1β (IL-1β) genotype in a Polish cohort of patients with RAS.
  
  
  METHODS
  One hundred and four patients with RAS were enrolled in the study and all were subject to an oral examination. The severity of RAS was assessed according to the clinical type of the disease, the number of lesions per flare-up and the length of intervals between the episodes. The genotyping of two SNPs of the IL-1β gene, IL-1β c.-511 T>C (rs16944) and IL-1β c.+3954 C>T (rs1143634), was carried out by genomic DNA isolated from blood samples and by using the PCR-RFLP approach. The results were statistically analysed with chi-square and Fisher's tests and Spearman's rank correlation, with P < 0.05 assumed to be a significance level (Statistica 10; StatSoft®, Kraków, Poland).
  
  
  RESULTS
  No statistically significant associations or correlations were found between the presence of *2 allele and minor, major or herpetiform RAS nor after the stratification using the RAS severity score and the classification based on the number of lesions per one flare-up. A weak correlation was found between the number of lesions per one flare-up and heterozygosity promoting the A type of RAS, characterized by a low number of eruptions.
  
  
  CONCLUSIONS
  The presence of IL-1 β *2 allele within the c.+3954 and c.-511 SNPs was found to be neither a significant risk factor for a higher incidence of any type of RAS, nor did it influence the disease severity and mode of recurrences.
authors.affiliation: Department of Oral Mucosa Diseases, University of Medical Sciences, Poznań, Poland.
authors.forename: Zuzanna
authors.initials: Z
authors.lastname: Ślebioda
authors.affiliation: Department of Oral Mucosa Diseases, University of Medical Sciences, Poznań, Poland.
authors.forename: Ewa
authors.initials: E
authors.lastname: Krawiecka
authors.affiliation: Department of Nucleic Acids Function, Institute of Human Genetics, Polish Academy of Sciences, Poznań, Poland.
authors.forename: Marta
authors.initials: M
authors.lastname: Rozmiarek
authors.affiliation: Department of Oral Mucosa Diseases, University of Medical Sciences, Poznań, Poland.
authors.forename: Elżbieta
authors.initials: E
authors.lastname: Szponar
authors.affiliation: Department of Nucleic Acids Function, Institute of Human Genetics, Polish Academy of Sciences, Poznań, Poland.
authors.forename: Anna
authors.initials: A
authors.lastname: Kowalska
authors.affiliation: Department of Oral Mucosa Diseases, University of Medical Sciences, Poznań, Poland.
authors.forename: Barbara
authors.initials: B
authors.lastname: Dorocka-Bobkowska
mesh_terms: D000328:Adult; D016022:Case-Control Studies; D005260:Female; D056726:Genetic Association Studies; D006801:Humans; D053583:Interleukin-1beta / Q000235:genetics*; D008297:Male; D011044:Poland; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide / Q000235:genetics; D012008:Recurrence; D013281:Stomatitis, Aphthous / Q000235:genetics*
chemical_list: D053583:Interleukin-1beta
keywords: genetic susceptibility; interleukin-1β; recurrent aphthous stomatitis