title: Allergin-1 inhibits TLR2-mediated mast cell activation and suppresses dermatitis.
abstract: TLR2 recognizes cell wall components of Staphylococcus aureus, which colonizes >90% of atopic eczematous skin lesions. The regulatory mechanisms of TLR2 signaling in the skin remain unclear. Allergin-1, an inhibitory immunoglobulin-like receptor containing an ITIM, is expressed on mast cells (MCs) and inhibits IgE-mediated anaphylaxis in mice. Here, we show that Allergin-1 inhibits TLR2-mediated activation of, and inflammatory cytokine production by, MCs in vitro Compared with wild-type mice, Allergin-1-deficient mice showed enhanced ear swelling with enhanced collagen deposition and greater Ly6G+neutrophil recruitment after intra-dermal injection of Pam2CSK4 into pinnae. Using Mas-TRECK mice, which is an MC deletion system based on il4 enhancer elements, we also demonstrated that Allergin-1 on MCs is responsible for the Pam2CSK4-induced ear swelling. These results suggest that Allergin-1 on skin MCs suppresses TLR2-induced dermatitis.
authors.affiliation: Department of Immunology, Faculty of Medicine and.
authors.forename: Satoshi
authors.initials: S
authors.lastname: Tsurusaki
authors.affiliation: Department of Immunology, Faculty of Medicine and tokothr@md.tsukuba.ac.jp.|Life Science Center of Tsukuba Advanced Research Alliance (TARA), University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8575, Japan.
authors.forename: Satoko
authors.initials: S
authors.lastname: Tahara-Hanaoka
authors.affiliation: Department of Immunology, Faculty of Medicine and.
authors.forename: Shohei
authors.initials: S
authors.lastname: Shibagaki
authors.affiliation: Department of Immunology, Faculty of Medicine and.
authors.forename: Shota
authors.initials: S
authors.lastname: Miyake
authors.affiliation: Research Center of Immunology, Tsukuba Institute, ONO Pharmaceutical Co., Ltd, 17-2 Wadai, Tsukuba, Ibaraki 300-4247, Japan.
authors.forename: Masamichi
authors.initials: M
authors.lastname: Imai
authors.affiliation: Research Center of Immunology, Tsukuba Institute, ONO Pharmaceutical Co., Ltd, 17-2 Wadai, Tsukuba, Ibaraki 300-4247, Japan.
authors.forename: Shiro
authors.initials: S
authors.lastname: Shibayama
authors.affiliation: Division of Molecular Pathology, Research Institute for Biomedical Science, Tokyo University of Science, 2669 Yamazaki, Noda, Chiba 278-0022, Japan.|Laboratory for Cytokine Regulation, RIKEN Center for Integrative Medical Sciences (IMS), 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama, Kanagawa 230-0045, Japan.
authors.forename: Masato
authors.initials: M
authors.lastname: Kubo
authors.affiliation: Department of Immunology, Faculty of Medicine and.|Life Science Center of Tsukuba Advanced Research Alliance (TARA), University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8575, Japan.
authors.forename: Akira
authors.initials: A
authors.lastname: Shibuya
mesh_terms: D000818:Animals; D003872:Dermatitis / Q000276:immunology* / Q000473:pathology; D008407:Mast Cells / Q000166:cytology / Q000276:immunology*; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D011971:Receptors, Immunologic / Q000172:deficiency / Q000276:immunology*; D051195:Toll-Like Receptor 2 / Q000037:antagonists & inhibitors* / Q000276:immunology
chemical_list: C551887:Milr1 protein, mouse; D011971:Receptors, Immunologic; C493486:Tlr2 protein, mouse; D051195:Toll-Like Receptor 2
keywords: TLR2; allergin-1; dermatitis; mast cell