title: The 5-chlorouracil:7-deazaadenine base pair as an alternative to the dT:dA base pair.
abstract: 5-Chloro-2'-deoxyuridine as a possible component of a chemically modified genome has been discussed in terms of its influence on duplex stability and DNA polymerase incorporation properties. The search for its counterpart among different deoxyadenosine analogs (7-deaza-, 8-aza- and 8-aza-7-deaza-2'-deoxyadenosines) showed that the stable duplex formation as well as the synthesis of long constructs, more than 2 kb, were successful with the 5-chloro-2'-deoxyuridine and 7-deaza-2'-deoxyadenosine combination and with Taq DNA polymerase.
authors.affiliation: KU Leuven, Rega Institute for Medical Research, Medicinal Chemistry, Minderbroedersstraat 10, BE-3000 Leuven, Belgium. piet.herdewijn@kuleuven.be.
authors.forename: E
authors.initials: E
authors.lastname: Eremeeva
authors.affiliation: KU Leuven, Rega Institute for Medical Research, Medicinal Chemistry, Minderbroedersstraat 10, BE-3000 Leuven, Belgium. piet.herdewijn@kuleuven.be.
authors.forename: M
authors.initials: M
authors.lastname: Abramov
authors.affiliation: iSSB, Genopole, CNRS, UEVE, Université Paris-Saclay, 5 rue Henri Desbruères, 91030 Evry Cedex, France.
authors.forename: P
authors.initials: P
authors.lastname: Marlière
authors.affiliation: KU Leuven, Rega Institute for Medical Research, Medicinal Chemistry, Minderbroedersstraat 10, BE-3000 Leuven, Belgium. piet.herdewijn@kuleuven.be and iSSB, Genopole, CNRS, UEVE, Université Paris-Saclay, 5 rue Henri Desbruères, 91030 Evry Cedex, France.
authors.forename: P
authors.initials: P
authors.lastname: Herdewijn
mesh_terms: D000225:Adenine / Q000031:analogs & derivatives* / Q000737:chemistry; D020029:Base Pairing; D004247:DNA / Q000737:chemistry*; D003839:Deoxyadenosines / Q000737:chemistry; D003857:Deoxyuridine / Q000031:analogs & derivatives* / Q000737:chemistry; D014372:Tubercidin / Q000031:analogs & derivatives* / Q000737:chemistry; D014498:Uracil / Q000031:analogs & derivatives* / Q000737:chemistry
chemical_list: C466641:8-aza-2'-deoxyadenosine; D003839:Deoxyadenosines; C043837:7-deazaadenine; C012244:5-chloro-2'-deoxyuridine; D014498:Uracil; C053222:2'-deoxytubercidin; C010889:5-chlorouracil; D004247:DNA; D000225:Adenine; D014372:Tubercidin; D003857:Deoxyuridine