title: OTX2 impedes self-renewal of porcine iPS cells through downregulation of NANOG expression.
abstract: The transcription factor Otx2 acts as a negative switch in the regulation of transition from naive to primed pluripotency in mouse pluripotent stem cells. However, the molecular features and function of porcine OTX2 have not been well elucidated in porcine-induced pluripotent stem cells (piPSCs). By studying high-throughput transcriptome sequencing and interfering endogenousOTX2expression, we demonstrate that OTX2 is able to downgrade the self-renewal of piPSCs.OTX2is highly expressed in porcine brain, reproductive tissues, and preimplantation embryos, but is undetectable in fibroblasts and most somatic tissues. However, the known piPSC lines reported previously produced different levels of OTX2 depending on the induction procedures and culture conditions. Overexpression of porcine OTX2 can reduce the percentage of alkaline phosphatase-positive colonies and downregulateNANOGandOCT4expression. In contrast, knockdown ofOTX2can significantly increase endogenous expressions ofNANOG,OCT4, andESRRB, and stabilize the pluripotent state of piPSCs. On the other hand, NANOG can directly bind to theOTX2promoter as shown in ChIP-seq data and repressOTX2promoter activity in a dose-dependent manner. These observations indicate that OTX2 and NANOG can form a negative feedback circuitry to regulate the pluripotency of porcine iPS cells.
authors.affiliation: Department of Animal Biotechnology, College of Veterinary Medicine, Northwest A&F University , Yangling, Shaanxi 712100, China.
authors.forename: Ning
authors.initials: N
authors.lastname: Wang
authors.affiliation: Department of Animal Biotechnology, College of Veterinary Medicine, Northwest A&F University , Yangling, Shaanxi 712100, China.
authors.forename: Yaxian
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Animal Biotechnology, College of Veterinary Medicine, Northwest A&F University , Yangling, Shaanxi 712100, China.
authors.forename: Youlong
authors.initials: Y
authors.lastname: Xie
authors.affiliation: Department of Animal Biotechnology, College of Veterinary Medicine, Northwest A&F University , Yangling, Shaanxi 712100, China.
authors.forename: Huayan
authors.initials: H
authors.lastname: Wang