title: Amyloid-graphene oxide as immobilization platform of Au nanocatalysts and enzymes for improved glucose-sensing activity.
abstract: Two-dimensional GO nanosheets and one-dimensional lysozyme nanofibrils were hybridized through electrostatic interaction to get a novel amyloid-GO composite, which promised a biocompatible immobilization platform for Au nanocatalysts as well as enzymes. The immobilization platform could load a large and tunable amount of Au NPs while maintaining their high catalytic activity. The immobilized catalysts showed high electrochemical behaviors, being ideal as glucose sensing systems. Furthermore, enzymes could also be immobilized on the residual bare surfaces of amyloid-GO, and served by a colorimetric method for a sensitive and selective analytical glucose-detecting platform. The introduction of amyloid fibrils with super large aspect ratios (>103) on GO nanosheets offers an unprecedented possibility of designing and developing novel biomimetic catalysts for broad applications in biotechnology.
authors.affiliation: CAS Key Laboratory of Bio-based Materials, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Songling Road 189, Qingdao 266101, PR China.
authors.forename: Xiaochen
authors.initials: X
authors.lastname: Wu
authors.affiliation: CAS Key Laboratory of Bio-based Materials, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Songling Road 189, Qingdao 266101, PR China.
authors.forename: Mingjie
authors.initials: M
authors.lastname: Li
authors.affiliation: Beijing Engineering Research Center of Process Pollution Control, Division of Environmental Technology and Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, PR China.
authors.forename: Zehui
authors.initials: Z
authors.lastname: Li
authors.affiliation: CAS Key Laboratory of Bio-based Materials, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Songling Road 189, Qingdao 266101, PR China.
authors.forename: Lili
authors.initials: L
authors.lastname: Lv
authors.affiliation: School of Materials Engineering, Shanghai University of Engineering Science, Longteng Road 333, Shanghai 201620, PR China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: CAS Key Laboratory of Bio-based Materials, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Songling Road 189, Qingdao 266101, PR China. Electronic address: licx@qibebt.ac.cn.
authors.forename: Chaoxu
authors.initials: C
authors.lastname: Li
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D003124:Colorimetry / Q000379:methods; D055664:Electrochemical Techniques / Q000379:methods; D004800:Enzymes, Immobilized / Q000737:chemistry; D005947:Glucose / Q000032:analysis*; D006046:Gold / Q000737:chemistry*; D006108:Graphite / Q000737:chemistry*; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D009113:Muramidase / Q000737:chemistry; D053761:Nanocomposites / Q000737:chemistry* / Q000648:ultrastructure; D057139:Nanofibers / Q000737:chemistry / Q000648:ultrastructure; D010087:Oxides / Q000737:chemistry*
chemical_list: D004800:Enzymes, Immobilized; D010087:Oxides; D006046:Gold; D006108:Graphite; D009113:Muramidase; D005947:Glucose
keywords: Amyloid nanofibrils; Au nanoparticles; Biocompatible immobilization platform; Biosensing; Graphene oxide