title: Establishment of induced pluripotent stem cell (iPSC) line from 55-year old male patient with hemorrhagic Moyamoya disease.
abstract: Peripheral blood mononuclear cells (PBMCs) were collected from 55-year old male patient with a confirmed diagnosis of hemorrhagic Moyamoya disease (MMD). PBMCs were reprogrammed using Sendai virus particles delivering the four Yamanaka factors. A footprint-free hiPSC line was characterized by the expression of pluripotency markers and a normal karyotype. These cells were able to give rise to Embryoid Bodies and to a progeny of differentiated cells belonging to the 3 germ layers. This hiPSC line represents a suitable tool for modelling in vitro MMD disease to investigate the cellular mechanisms underlying the occurrence of this pathology.
authors.affiliation: Laboratory of Stem Cell Biology, Centre for Integrative Biology-CIBIO, Università degli Studi di Trento, Trento, Italy.
authors.forename: Marina
authors.initials: M
authors.lastname: Cardano
authors.affiliation: Laboratory of Stem Cell Biology, Centre for Integrative Biology-CIBIO, Università degli Studi di Trento, Trento, Italy. Electronic address: fmar@uni-bonn.de.
authors.forename: Fabio
authors.initials: F
authors.lastname: Marsoner
authors.affiliation: Laboratory of Stem Cell Biology, Centre for Integrative Biology-CIBIO, Università degli Studi di Trento, Trento, Italy; Department of Health Sciences, Università Degli Studi di Milano, Milan, Italy; Department of Mental Health, San Paolo Hospital, Milan, Italy.
authors.forename: Matteo
authors.initials: M
authors.lastname: Marcatili
authors.affiliation: Chromatin Dynamics Unit, San Raffaele University and Research Institute, Milan, Italy. Electronic address: tk2708@cumc.columbia.edu.
authors.forename: Thodoris
authors.initials: T
authors.lastname: Karnavas
authors.affiliation: Laboratory of Stem Cell Biology, Centre for Integrative Biology-CIBIO, Università degli Studi di Trento, Trento, Italy.
authors.forename: Jacopo
authors.initials: J
authors.lastname: Zasso
authors.affiliation: Department of Neuroscience and Surgery of the Nervous System, Papa Giovanni XXIII Hospital, Bergamo, Italy. Electronic address: l.lanterna@gmail.com.
authors.forename: Luigi Andrea
authors.initials: LA
authors.lastname: Lanterna
authors.affiliation: Laboratory of Stem Cell Biology, Centre for Integrative Biology-CIBIO, Università degli Studi di Trento, Trento, Italy. Electronic address: Luciano.Conti@unitn.it.
authors.forename: Luciano
authors.initials: L
authors.lastname: Conti
mesh_terms: D002454:Cell Differentiation; D002460:Cell Line; D065150:Cellular Reprogramming; D058732:Embryoid Bodies / Q000166:cytology / Q000378:metabolism; D005822:Genetic Vectors / Q000235:genetics / Q000378:metabolism; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000166:cytology* / Q000378:metabolism; D059785:Karyotype; D007963:Leukocytes, Mononuclear / Q000166:cytology; D008297:Male; D008856:Microscopy, Fluorescence; D008875:Middle Aged; D009072:Moyamoya Disease / Q000378:metabolism / Q000473:pathology*; D029082:Sendai virus / Q000235:genetics; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism
chemical_list: D014157:Transcription Factors