title: Selenium levels in human breast carcinoma tissue are associated with a common polymorphism in the gene for SELENOP (Selenoprotein P).
abstract: Selenium supplementation of the diets of rodents has consistently been shown to suppress mammary carcinogenesis and some, albeit not all, human epidemiological studies have indicated an inverse association between selenium and breast cancer risk. In order to better understand the role selenium plays in breast cancer, 30 samples of tumor tissue were obtained from women with breast cancer and analyzed for selenium concentration, the levels of several selenium-containing proteins and the levels of the MnSOD anti-oxidant protein. Polymorphisms within the genes for these same proteins were determined from DNA isolated from the tissue samples. There was a wide range of selenium in these tissues, ranging from 24 to 854ng/gm. The selenium levels in the tissues were correlated to the genotype of the SELENOP selenium carrier protein, but not to other proteins whose levels have been reported to be responsive to selenium availability, including GPX1, SELENOF and SBP1. There was an association between a polymorphism in the gene for MnSOD and the levels of the encoded protein. These studies were the first to examine the relationship between selenium levels, genotypes and protein levels in human tissues. Furthermore, the obtained data provide evidence for the need to obtain data about the effects of selenium in breast cancer by examining samples from that particular tissue type.
authors.affiliation: Department of Pathology, College of Medicine, University of Illinois at Chicago, Chicago, IL, USA. Electronic address: dekoue2@uic.edu.
authors.forename: Dede N
authors.initials: DN
authors.lastname: Ekoue
authors.affiliation: Department of Pathology, College of Medicine, University of Illinois at Chicago, Chicago, IL, USA. Electronic address: szaichik@yahoo.com.
authors.forename: Sofia
authors.initials: S
authors.lastname: Zaichick
authors.affiliation: Department of Pathology, College of Medicine, University of Illinois at Chicago, Chicago, IL, USA. Electronic address: klaravn@uic.edu.
authors.forename: Klara
authors.initials: K
authors.lastname: Valyi-Nagy
authors.affiliation: USDA-ARS, Grand Forks Human Nutrition Research Center, Grand Forks, ND, USA. Electronic address: Matthew.Picklo@ars.usda.gov.
authors.forename: Matthew
authors.initials: M
authors.lastname: Picklo
authors.affiliation: USDA-ARS, Grand Forks Human Nutrition Research Center, Grand Forks, ND, USA. Electronic address: Craig.Lacher@ars.usda.gov.
authors.forename: Craig
authors.initials: C
authors.lastname: Lacher
authors.affiliation: Department of Medicine, College of Medicine, University of Illinois at Chicago, Chicago, IL, USA. Electronic address: khoski@uic.edu.
authors.forename: Kent
authors.initials: K
authors.lastname: Hoskins
authors.affiliation: Department of Surgery, College of Medicine, University of Illinois at Chicago, Chicago, IL, USA. Electronic address: warso@uic.edu.
authors.forename: Michael A
authors.initials: MA
authors.lastname: Warso
authors.affiliation: Department of Pathology, College of Medicine, University of Illinois at Chicago, Chicago, IL, USA; Department of Medicine, College of Medicine, University of Illinois at Chicago, Chicago, IL, USA. Electronic address: mbonini@uic.edu.
authors.forename: Marcelo G
authors.initials: MG
authors.lastname: Bonini
authors.affiliation: Department of Pathology, College of Medicine, University of Illinois at Chicago, Chicago, IL, USA. Electronic address: adiamond@uic.edu.
authors.forename: Alan M
authors.initials: AM
authors.lastname: Diamond
mesh_terms: D000328:Adult; D000368:Aged; D001943:Breast Neoplasms / Q000737:chemistry / Q000175:diagnosis / Q000235:genetics* / Q000378:metabolism*; D005260:Female; D006801:Humans; D008875:Middle Aged; D011110:Polymorphism, Genetic / Q000235:genetics*; D012643:Selenium / Q000032:analysis* / Q000378:metabolism; D051149:Selenoprotein P / Q000235:genetics*; D055815:Young Adult
chemical_list: D051149:Selenoprotein P; D012643:Selenium
keywords: Breast cancer; GPX1; Glutathione peroxidase-1; Manganese superoxide dismutase; MnSOD; Polymorphisms; SELENOP; Selenium; Selenoprotein P