title: Production of Computationally Designed Small Soluble- and Membrane-Proteins: Cloning, Expression, and Purification.
abstract: This book chapter focuses on expression and purification of computationally designed small soluble proteins and membrane proteins that are ordinarily difficult to express in good amounts for experiments. Over-expression of such proteins can be achieved by using the solubility tag such as maltose binding protein (MBP), Thioredoxin (Trx), and Gultathione-S-transferase (GST) fused to the protein of interest. Here, we describe and provide the protocols for cloning, expression and purification of such proteins using the solubility tag.
authors.affiliation: School of Biological Sciences, National Institute of Science Education and Research, Jatni, Khurda, 752050, Odisha, India.|Homi Bhabha National Institute, Training School Complex, Anushakti Nagar, Mumbai, 400094, India.
authors.forename: Barsa
authors.initials: B
authors.lastname: Tripathy
authors.affiliation: School of Biological Sciences, National Institute of Science Education and Research, Jatni, Khurda, 752050, Odisha, India. rudresh.acharya@niser.ac.in.|Homi Bhabha National Institute, Training School Complex, Anushakti Nagar, Mumbai, 400094, India. rudresh.acharya@niser.ac.in.
authors.forename: Rudresh
authors.initials: R
authors.lastname: Acharya
mesh_terms: D003001:Cloning, Molecular; D019295:Computational Biology / Q000379:methods; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D058619:Maltose-Binding Proteins / Q000235:genetics; D008565:Membrane Proteins / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification*; D015202:Protein Engineering / Q000379:methods; D011993:Recombinant Fusion Proteins; D012995:Solubility
chemical_list: D058619:Maltose-Binding Proteins; D008565:Membrane Proteins; D011993:Recombinant Fusion Proteins
keywords: Cloning; Designed proteins; Protein expression and purification; Small proteins; Solubility tags