title: Rapid, Sensitive Detection of Bartonella quintana by Loop-Mediated Isothermal Amplification of the groEL Gene.
abstract: Trench fever, caused byBartonella quintana, is recognized as a re-emerging and neglected disease. Rapid and sensitive detection approaches are urgently required to monitor and help controlB. quintanainfections. Here, loop-mediated isothermal amplification (LAMP), which amplifies target DNA at a fixed temperature with high sensitivity, specificity and rapidity, was employed to detectB. quintana. Thirty-six strains, including 10B. quintana, 13 otherBartonellaspp., and 13 other common pathogens, were applied to verify and evaluate the LAMP assay. The specificity of the LAMP assay was 100%, and the limit of detection was 125 fg/reaction. The LAMP assay was compared with qPCR in the examination of 100 rhesus and 20 rhesus-feeder blood samples; the diagnostic accuracy was found to be 100% when LAMP was compared to qPCR, but the LAMP assay was significantly more sensitive (p< 0.05). Thus, LAMP methodology is a useful for diagnosis of trench fever in humans and primates, especially in low-resource settings, because of its rapid, sensitive detection that does not require sophisticated equipment.
authors.affiliation: Clinical Laboratory of Peking University Shougang Hospital, Beijing100144, China. shoukuihua@163.com.|State Key Laboratory of Infectious Disease Prevention and Control, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Changping, Beijing 102206, China. shoukuihua@163.com.
authors.forename: Shoukui
authors.initials: S
authors.lastname: Hu
authors.affiliation: School of Tropical and Laboratory Medicine, Hainan Medical University, Haikou 571199, China. xiaoniuzhb@163.com.|School of Life Science, Shanxi University, Taiyuan 030006, China. xiaoniuzhb@163.com.
authors.forename: Lina
authors.initials: L
authors.lastname: Niu
authors.affiliation: Clinical Laboratory of Peking University Shougang Hospital, Beijing100144, China. Luolyaduo@163.com.
authors.forename: Lijuan
authors.initials: L
authors.lastname: Luo
authors.affiliation: State Key Laboratory of Infectious Disease Prevention and Control, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Changping, Beijing 102206, China. songxiuping@icdc.cn.
authors.forename: Xiuping
authors.initials: X
authors.lastname: Song
authors.affiliation: State Key Laboratory of Infectious Disease Prevention and Control, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Changping, Beijing 102206, China. jmsun@cdc.zj.cn.|Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou 310051, China. jmsun@cdc.zj.cn.
authors.forename: Jimin
authors.initials: J
authors.lastname: Sun
authors.affiliation: State Key Laboratory of Infectious Disease Prevention and Control, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Changping, Beijing 102206, China. liuqiyong@icdc.cn.
authors.forename: Qiyong
authors.initials: Q
authors.lastname: Liu
mesh_terms: D000818:Animals; D018413:Bartonella quintana / Q000235:genetics / Q000302:isolation & purification* / Q000472:pathogenicity; D018834:Chaperonin 60 / Q000097:blood* / Q000235:genetics; D006801:Humans; D008253:Macaca mulatta / Q000097:blood / Q000382:microbiology; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods; D014205:Trench Fever / Q000097:blood* / Q000235:genetics / Q000382:microbiology
chemical_list: D018834:Chaperonin 60
keywords: Bartonella; detection; re-emerging pathogen; trench fever