title: TheArabidopsis ANGUSTIFOLIA3-YODAGene Cascade Induces Anthocyanin Accumulation by Regulating Sucrose Levels.
abstract: Anthocyanin accumulation specifically depends on sucrose (Suc) signaling/levels. However, the gene cascades specifically involved in the Suc signaling/level-mediated anthocyanin biosynthetic pathway are still unknown.Arabidopsis ANGUSTIFOLIA3(AN3), a transcription coactivator, is involved in the regulation of leaf shape and drought tolerance. Recently, anAN3-CONSTITUTIVE PHOTOMORPHOGENIC 1gene cascade has been reported to regulate the light signaling-mediated anthocyanin accumulation. Target gene analysis indicates thatAN3is associated with theYODA(YDA) promoter, a mitogen-activated protein kinase kinase kinase,in vivofor inducing anthocyanin accumulation. Indeed, loss-of-function mutants ofYDAshowed significantly increased anthocyanin accumulation.YDAmutation can also suppress the decrease inan3-4anthocyanin accumulation. Further analysis indicates that the mutations ofAN3andYDAdisrupt the normal Suc levels because of the changes of invertase activity in mutants ofan3oryda, which in turn induces the alterations of anthocyanin accumulation in mutants ofan3orydavia unknown regulatory mechanisms.
authors.affiliation: The Key Laboratory of Biotechnology for Medicinal Plant of Jiangsu Province, School of Life Science, Jiangsu Normal UniversityXuzhou, China; Centre for Transformational Biotechnology of Medicinal and Food Plants, Jiangsu Normal University - Edinburgh UniversityXuzhou, China.
authors.forename: Lai-Sheng
authors.initials: LS
authors.lastname: Meng
authors.affiliation: The Key Laboratory of Biotechnology for Medicinal Plant of Jiangsu Province, School of Life Science, Jiangsu Normal UniversityXuzhou, China; Centre for Transformational Biotechnology of Medicinal and Food Plants, Jiangsu Normal University - Edinburgh UniversityXuzhou, China.
authors.forename: Ying-Qiu
authors.initials: YQ
authors.lastname: Li
authors.affiliation: The Key Laboratory of Biotechnology for Medicinal Plant of Jiangsu Province, School of Life Science, Jiangsu Normal UniversityXuzhou, China; Centre for Transformational Biotechnology of Medicinal and Food Plants, Jiangsu Normal University - Edinburgh UniversityXuzhou, China.
authors.forename: Meng-Qian
authors.initials: MQ
authors.lastname: Liu
authors.affiliation: The Key Laboratory of Biotechnology for Medicinal Plant of Jiangsu Province, School of Life Science, Jiangsu Normal UniversityXuzhou, China; Centre for Transformational Biotechnology of Medicinal and Food Plants, Jiangsu Normal University - Edinburgh UniversityXuzhou, China.
authors.forename: Ji-Hong
authors.initials: JH
authors.lastname: Jiang
keywords: AN3/GIF1; Arabidopsis; YODA (YDA); anthocyanin accumulations; sucrose levels