title: Paper-based CRP Monitoring Devices.
abstract: Here, we discuss the development of a paper-based diagnostic device that is inexpensive, portable, easy-to-use, robust, and capable of running simultaneous tests to monitor a relevant inflammatory protein for clinical diagnoses i.e. C-reactive protein (CRP). In this study, we first attempted to make a paper-based diagnostic device via the wax printing method, a process that was used in previous studies. This device has two distinct advantages: 1) reduced manufacturing and assay costs and operation duration via using wax printing method to define hydrophobic boundaries (for fluidic devices or general POC devices); and, 2) the hydrophilicity of filter paper, which is used to purify and chromatographically correct interference caused by whole blood components with a tiny amount of blood sample (only 5 μL). Diagnosis was based on serum stain length retained inside the paper channels of our device. This is a balanced function between surface tension and chromatographic force following immune reactions (CRP assays) with a paper-embedded biomarker.
authors.affiliation: Institute of Nanoengineering and Microsystems, National Tsing Hua University, No. 101, Section 2, Kuang-Fu Road, Hsinchu 300, Taiwan.
authors.forename: Shang-Chi
authors.initials: SC
authors.lastname: Lin
authors.affiliation: Department of Orthopaedics, Taichung Veterans General Hospital, No.1650, Sec. 4, Taiwan Blvd., Xitun Dist., Taichung City 407, Taiwan.
authors.forename: Chung-Yuh
authors.initials: CY
authors.lastname: Tzeng
authors.affiliation: Department of Orthopedic Surgery, Bone and Joint Research Center, Chang Gung Memorial Hospital at Linkou, College of Medicine, Chang Gung University, Taoyuan, Taiwan.
authors.forename: Po-Liang
authors.initials: PL
authors.lastname: Lai
authors.affiliation: Institute of Nanoengineering and Microsystems, National Tsing Hua University, No. 101, Section 2, Kuang-Fu Road, Hsinchu 300, Taiwan.|Department of Ophthalmology, Taichung Veterans General Hospital, No. 1650, Sec. 4, Taiwan Blvd., Xitun Dist., Taichung City 407, Taiwan.
authors.forename: Min-Yen
authors.initials: MY
authors.lastname: Hsu
authors.affiliation: Department of Engineering and System Science, National Tsing Hua University, Hsinchu 300, Taiwan.
authors.forename: Hsueh-Yao
authors.initials: HY
authors.lastname: Chu
authors.affiliation: Department of Engineering and System Science, National Tsing Hua University, Hsinchu 300, Taiwan.
authors.forename: Fan-Gang
authors.initials: FG
authors.lastname: Tseng
authors.affiliation: Institute of Biomedical Engineering, National Tsing Hua University, No. 101, Section 2, Kuang-Fu Road, Hsinchu 300, Taiwan.
authors.forename: Chao-Min
authors.initials: CM
authors.lastname: Cheng