title: Transcriptomic analysis of gene expression profiles of stomach carcinoma reveal abnormal expression of mitotic components.
abstract: OBJECTIVE
  In order to explore the etiology of gastric cancer on global gene expression level, we developed advanced bioinformatic analysis to investigate the variations of global gene expression and the interactions among them.
  
  
  METHODS
  We downloaded the dataset GSE63288 from Gene Expression Omnibus (GEO) database which included 22 human gastric cancer and 22 healthy control samples. We identified the differential expression genes, and explored the Gene ontology (GO) and pathways of the differentially expressed genes. Furthermore, integrative interaction network and co-expression network were employed to identify the key genes which may contribute to gastric cancer progression.
  
  
  RESULTS
  The results indicated that 5 kinases including BUB1, TTK protein kinase, Citron Rho-interacting kinase (CIT), ZAK and NEK2 were upregulated in gastric cancer. Interestingly, BUB1, TTK, CIT and NEK2 have shown high expression similarities and bound with each other, and participated in multiple phases of mitosis. Moreover, a subnet of co-expression genes e.g. KIF14, PRC1, CENPF and CENPI was also involved in mitosis which was functionally coupled with the kinases above. By validation assays, the results indicated that CIT, PRC1, TTK and KIF14 were significantly upregulated in gastric cancer.
  
  
  CONCLUSIONS
  These evidences have suggested that aberrant expression of these genes may drive gastric cancer including progression, invasion and metastasis. Although the causal relationships between gastric cancer and the genes are still lacking, it was reasonable to take them as biomarkers for diagnosis of gastric cancer.
authors.affiliation: The hepatobiliary department, The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, Wenzhou 325000, Zhejiang, China.
authors.forename: Hongfei
authors.initials: H
authors.lastname: Tong
authors.affiliation: The hepatobiliary department, The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, Wenzhou 325000, Zhejiang, China.
authors.forename: Jisheng
authors.initials: J
authors.lastname: Wang
authors.affiliation: The hepatobiliary department, The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, Wenzhou 325000, Zhejiang, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Chen
authors.affiliation: The hepatobiliary department, The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, Wenzhou 325000, Zhejiang, China.
authors.forename: Zhaohong
authors.initials: Z
authors.lastname: Wang
authors.affiliation: The hepatobiliary department, The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, Wenzhou 325000, Zhejiang, China.
authors.forename: Henwei
authors.initials: H
authors.lastname: Fan
authors.affiliation: The hepatobiliary department, The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, Wenzhou 325000, Zhejiang, China. Electronic address: chinanister@tom.com.
authors.forename: Zhonglin
authors.initials: Z
authors.lastname: Ni
mesh_terms: D002277:Carcinoma / Q000378:metabolism* / Q000473:pathology; D016022:Case-Control Studies; D018797:Cell Cycle Proteins / Q000378:metabolism; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D019295:Computational Biology; D030541:Databases, Genetic; D018450:Disease Progression; D005753:Gastric Mucosa / Q000378:metabolism; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins / Q000378:metabolism; D020930:MAP Kinase Kinase Kinases; D008938:Mitosis; D000072379:NIMA-Related Kinases / Q000378:metabolism; D011494:Protein Kinases / Q000378:metabolism; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism; D011505:Protein-Tyrosine Kinases / Q000378:metabolism; D008941:Spindle Apparatus; D013274:Stomach Neoplasms / Q000378:metabolism* / Q000473:pathology; D059467:Transcriptome
chemical_list: D018797:Cell Cycle Proteins; D047908:Intracellular Signaling Peptides and Proteins; D011494:Protein Kinases; C115635:citron-kinase; D011505:Protein-Tyrosine Kinases; C584277:BUB1 protein, human; C089441:NEK2 protein, human; D000072379:NIMA-Related Kinases; D017346:Protein Serine-Threonine Kinases; D020930:MAP Kinase Kinase Kinases; C413047:MAP3K20 protein, human; C075840:TTK protein, human
keywords: Co-expression network; Gastric cancer; Gene interaction network; Mitosis; Transcriptomic analysis