title: Characterization of Xylanase and Cellulase Produced by a Newly Isolated Aspergillus fumigatus N2 and Its Efficient Saccharification of Barley Straw.
abstract: Aspergillus fumigatus N2 was isolated from decaying wood. This strain produces extracellular xylanases and cellulases. The highest xylanase (91.9 U/mL) and CMCase (5.61 U/mL) activity was produced when 1% barley straw was used as the carbon source. The optimum pH and temperature for xylanase activity were 6.0 and 65 °C, respectively. CMCase revealed maximum activity at pH 4.0 and in the range of 65 °C. The FPase was optimally active at pH 5.0 and 60 °C. The zymograms produced by the SDS-PAGE resolution of the crude enzymes indicated that multiple enzymes were secreted into the fermentation supernatant. Five bands of proteins with xylanase activity and four bands of proteins with endoglucanase were observed in the zymogram gel. The crude enzymes were used in the barley straw saccharification; an additive effect was observed when the commercial cellulase was added as supplement.
authors.affiliation: State Key Laboratory of Rice Biology and Institute of Insect Science, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, China.|Department of Biology, Lakehead University, Thunder Bay, ON, P7B 5E1, Canada.
authors.forename: Chaoyang
authors.initials: C
authors.lastname: Lin
authors.affiliation: State Key Laboratory of Rice Biology and Institute of Insect Science, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, China.
authors.forename: Zhicheng
authors.initials: Z
authors.lastname: Shen
authors.affiliation: Department of Biology, Lakehead University, Thunder Bay, ON, P7B 5E1, Canada. wqin@lakeheadu.ca.
authors.forename: Wensheng
authors.initials: W
authors.lastname: Qin
mesh_terms: D001232:Aspergillus fumigatus / Q000201:enzymology* / Q000254:growth & development / Q000302:isolation & purification; D044602:Cellulases / Q000096:biosynthesis* / Q000737:chemistry; D043364:Endo-1,4-beta Xylanases / Q000096:biosynthesis*; D005656:Fungal Proteins / Q000096:biosynthesis* / Q000737:chemistry; D001467:Hordeum; D006863:Hydrogen-Ion Concentration
chemical_list: D005656:Fungal Proteins; D044602:Cellulases; D043364:Endo-1,4-beta Xylanases
keywords: Aspergillus fumigatus; Cellulase; Xylanase