title: The effects of hydroxychloroquine on endothelial dysfunction.
abstract: Hydroxychloroquine is an anti-malarial drug which, due to its anti-inflammatory and immunomodulatory effects, is widely used for the treatment of autoimmune diseases. In a model of systemic lupus erythematosus hydroxychloroquine has been shown to exert protective endothelial effects. In this study, we aimed to investigate whether hydroxychloroquine was endothelial protective in an in vitro model of TNF-α and preeclamptic serum induced dysfunction. We showed that hydroxychloroquine significantly reduced the production of TNF-α and preeclamptic serum induced endothelin-1 (ET-1). Hydroxychloroquine also significantly mitigated TNF-α induced impairment of angiogenesis. These findings support the further assessment of hydroxychloroquine as an adjuvant therapy in preeclampsia.
authors.affiliation: The Ritchie Centre, Hudson Institute of Medical Research, 27-31 Wright Street, Clayton 3168, Melbourne, VIC, Australia; Department of Obstetrics and Gynaecology, School of Clinical Sciences, Monash University, Monash Medical Centre, 246 Clayton Road, Clayton 3168, Melbourne, VIC, Australia; Department of Obstetrics and Gynaecology, Faculty of Medicine, National University of Malaysia, Jalan Yaacob Latiff, Bandar Tun Razak, 56000 Cheras, Kuala Lumpur, Malaysia.
authors.forename: Rahana
authors.initials: R
authors.lastname: Rahman
authors.affiliation: Department of Medicine, School of Clinical Sciences, Monash University, Monash Medical Centre, 246 Clayton Road, Clayton 3168, Melbourne, VIC, Australia.
authors.forename: Padma
authors.initials: P
authors.lastname: Murthi
authors.affiliation: The Ritchie Centre, Hudson Institute of Medical Research, 27-31 Wright Street, Clayton 3168, Melbourne, VIC, Australia.
authors.forename: Harmeet
authors.initials: H
authors.lastname: Singh
authors.affiliation: The Ritchie Centre, Hudson Institute of Medical Research, 27-31 Wright Street, Clayton 3168, Melbourne, VIC, Australia; Department of Obstetrics and Gynaecology, School of Clinical Sciences, Monash University, Monash Medical Centre, 246 Clayton Road, Clayton 3168, Melbourne, VIC, Australia.
authors.forename: Seshini
authors.initials: S
authors.lastname: Gurusinghe
authors.affiliation: The Ritchie Centre, Hudson Institute of Medical Research, 27-31 Wright Street, Clayton 3168, Melbourne, VIC, Australia; Department of Obstetrics and Gynaecology, School of Clinical Sciences, Monash University, Monash Medical Centre, 246 Clayton Road, Clayton 3168, Melbourne, VIC, Australia.
authors.forename: Joanne C
authors.initials: JC
authors.lastname: Mockler
authors.affiliation: The Ritchie Centre, Hudson Institute of Medical Research, 27-31 Wright Street, Clayton 3168, Melbourne, VIC, Australia.
authors.forename: Rebecca
authors.initials: R
authors.lastname: Lim
authors.affiliation: The Ritchie Centre, Hudson Institute of Medical Research, 27-31 Wright Street, Clayton 3168, Melbourne, VIC, Australia; Department of Obstetrics and Gynaecology, School of Clinical Sciences, Monash University, Monash Medical Centre, 246 Clayton Road, Clayton 3168, Melbourne, VIC, Australia. Electronic address: euan.wallace@monash.edu.
authors.forename: Euan M
authors.initials: EM
authors.lastname: Wallace
mesh_terms: D000962:Antimalarials / Q000494:pharmacology*; D019332:Endothelin-1 / Q000096:biosynthesis*; D005260:Female; D061307:Human Umbilical Vein Endothelial Cells / Q000187:drug effects* / Q000378:metabolism; D006801:Humans; D006886:Hydroxychloroquine / Q000494:pharmacology*; D018919:Neovascularization, Physiologic / Q000187:drug effects; D011225:Pre-Eclampsia / Q000097:blood; D011247:Pregnancy; D061251:Primary Cell Culture; D044967:Serum; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis* / Q000494:pharmacology
chemical_list: D000962:Antimalarials; D019332:Endothelin-1; D014409:Tumor Necrosis Factor-alpha; D006886:Hydroxychloroquine
keywords: Endothelial dysfunction; Endothelin-1; Hydroxychloroquine; Preeclampsia; Preeclamptic serum; TNF-α