title: Critical role of the proton-dependent oligopeptide transporter (POT) in the cellular uptake of the peptidyl nucleoside antibiotic, blasticidin S.
abstract: Blasticidin S (BlaS) interferes in the cell growth of both eukaryotes and prokaryotes. Its mode of action as a protein synthesis inhibitor has been investigated extensively. However, the mechanism of BlaS transport into the target cells is not understood well. Here, we show that Ptr2, a member of the proton-dependent oligopeptide transporter (POT) family, is responsible for the uptake of BlaS in yeasts Schizosaccharomyces pombe and Saccharomyces cerevisiae. Notably, some mutants of Ptr2 that are dysfunctional in dipeptide uptake were still competent to transport BlaS. Mouse-derived oligopeptide transporter PepT1 conferred BlaS sensitivity in the S. cerevisiae ptr2∆ mutant. Furthermore, bacterial POT family proteins also potentiated the BlaS sensitivity of E. coli. The role of the POT family oligopeptide transporters in the uptake of BlaS is conserved across species from bacteria to mammals.
authors.affiliation: Center for Gene Science, Hiroshima University, 1-4-2 Kagamiyama, Higashi-Hiroshima 739-8527, Japan. Electronic address: kkita@hiroshima-u.ac.jp.
authors.forename: Kenji
authors.initials: K
authors.lastname: Kitamura
authors.affiliation: Center for Gene Science, Hiroshima University, 1-4-2 Kagamiyama, Higashi-Hiroshima 739-8527, Japan.
authors.forename: Eldaa Zefany Banami
authors.initials: EZ
authors.lastname: Kinsui
authors.affiliation: Department of Chemistry and Biological Science, College of Science and Engineering, Aoyama Gakuin University, Sagamihara 252-5258, Japan.
authors.forename: Fumiyoshi
authors.initials: F
authors.lastname: Abe
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000378:metabolism*; D026901:Membrane Transport Proteins / Q000502:physiology*; D051379:Mice; D009705:Nucleosides / Q000378:metabolism; D000074011:Peptide Transporter 1; D012441:Saccharomyces cerevisiae / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000502:physiology*; D027981:Symporters / Q000502:physiology
chemical_list: D000900:Anti-Bacterial Agents; D026901:Membrane Transport Proteins; D009705:Nucleosides; C093495:PTR2 protein, S cerevisiae; D000074011:Peptide Transporter 1; D029701:Saccharomyces cerevisiae Proteins; C489941:Slc15a1 protein, mouse; D027981:Symporters; C004500:blasticidin S
keywords: Blasticidin S; Oligopeptide transporter; POT family; Peptidyl nucleoside; Ptr2; Yeast