title: Relationships between High-mobility Group Protein B1 and Triggering Receptor Expressed on Myeloid Cells Concentrations in Gingival Crevicular Fluid and Chronic Periodontitis.
abstract: One of the inflammatory mediators which is secreted by inflammatory cells is high-mobility group protein B1 (HMGB1). Interaction of HMGB1 and toll-like receptors (TLRs) leads to increased production of inflammatory cytokines. On the other hand, it was shown that triggering receptor expressed on myeloid cells (TREM-1) also can be activated by TLRs, and its soluble form (sTREM-1) can be formed by cleaving of membrane-bound form of TREM-1 proteinases. Since there is not enough knowledge about the precise role of HMGB1 and sTREM-1 in periodontal diseases, the aim of this study was to evaluate the concentration of HMGB1 and sTREM-1 in gingival crevicular fluid (GCF) samples of patients with chronic periodontitis. Gingival crevicular fluid (GCF) samples were obtained from a total of 24 individuals with clinically healthy gingiva and 24 patients with moderate to severe chronic periodontitis. For collecting GCF samples, periopapers were placed at the entrance of the crevice and left in position for 30 seconds. Then, they were stored at -80°C. Enzyme-linked immunosorbent assay (ELISA) was used for measuring the concentration of HMGB1 and sTREM-1 in GCF samples. The concentration of HMGB1 (p<0.001) and sTREM-1 (p<0.017), was significantly higher in chronic periodontitis group. In addition, there was a significant positive correlation between HMGB1 and sTREM-1 concentration in chronic periodontitis group (p<0.05). We also found significant positive correlation between PD (Pocket depth) and the concentration of HMGB1 (p<0.001) and sTREM-1 (p<0.015). It is concluded that both HMGB1 and sTREM-1 are released during the inflammatory response of periodontal tissues and they can promote inflammatory process, which leads to tissue destruction.
authors.affiliation: Dental Research Center of Tehran University of Medical Sciences, Tehran, Iran AND Department of Periodontics, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Mojgan
authors.initials: M
authors.lastname: Paknejad
authors.affiliation: Department of Immunology, Medical School, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
authors.forename: Mandana
authors.initials: M
authors.lastname: Sattari
authors.affiliation: Faculty of Dentistry, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Zohreh
authors.initials: Z
authors.lastname: Roozbahani
authors.affiliation: Faculty of Dentistry, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Morteza
authors.initials: M
authors.lastname: Ershadi
authors.affiliation: Dental School, Islamic Azad University, Tehran, Iran.
authors.forename: Ali
authors.initials: A
authors.lastname: Mehrfard
mesh_terms: D000328:Adult; D055113:Chronic Periodontitis / Q000276:immunology* / Q000473:pathology; D005260:Female; D005881:Gingiva / Q000276:immunology* / Q000473:pathology; D024243:HMGB1 Protein / Q000276:immunology*; D006801:Humans; D008297:Male; D008562:Membrane Glycoproteins / Q000276:immunology*; D008875:Middle Aged; D011971:Receptors, Immunologic / Q000276:immunology*; D000073871:Triggering Receptor Expressed on Myeloid Cells-1
chemical_list: D024243:HMGB1 Protein; C585491:HMGB1 protein, human; D008562:Membrane Glycoproteins; D011971:Receptors, Immunologic; C408842:TREM1 protein, human; D000073871:Triggering Receptor Expressed on Myeloid Cells-1
keywords: Alarmins; Chronic periodontitis; DAMP; HMGB1; High-mobility group protein; Inflammation; sTREM1