title: Rapid detection of Hendra virus antibodies: an integrated device with nanoparticle assay and chaotic micromixing.
abstract: Current diagnosis of infectious diseases such as Hendra virus (HeV) relies mostly on laboratory-based tests. There is an urgent demand for rapid diagnosis technology to detect and identify these diseases in humans and animals so that disease spread can be controlled. In this study, an integrated lab-on-a-chip device using a magnetic nanoparticle immunoassay is developed. The key features of the device are the chaotic fluid mixing, achieved by magnetically driven motion of nanoparticles with the optimal mixing protocol developed using chaotic transport theory, and the automatic liquid handling system for loading reagents and samples. The device has been demonstrated to detect Hendra virus antibodies in dilute horse serum samples within a short time of 15 minutes and the limit of detection is about 0.48 ng ml-1. The device platform can potentially be used for field detection of viruses and other biological and chemical substances.
authors.affiliation: CSIRO Manufacturing, Private Bag 10, Clayton, Melbourne, VIC 3169, Australia. zhuyonggang@hitsz.edu.cn.
authors.forename: K
authors.initials: K
authors.lastname: Petkovic
authors.affiliation: CSIRO Manufacturing, Private Bag 10, Clayton, Melbourne, VIC 3169, Australia. zhuyonggang@hitsz.edu.cn and Swinburne University of Technology, Hawthorn, VIC 3122, Australia and Monash University, Clayton, VIC 3800, Australia.
authors.forename: G
authors.initials: G
authors.lastname: Metcalfe
authors.affiliation: CSIRO Manufacturing, Private Bag 10, Clayton, Melbourne, VIC 3169, Australia. zhuyonggang@hitsz.edu.cn and Harbin Institute of technology (Shenzhen), Shenzhen, Guangdong 518055, China.
authors.forename: H
authors.initials: H
authors.lastname: Chen
authors.affiliation: CSIRO Manufacturing, Private Bag 10, Clayton, Melbourne, VIC 3169, Australia. zhuyonggang@hitsz.edu.cn.
authors.forename: Y
authors.initials: Y
authors.lastname: Gao
authors.affiliation: CSIRO Manufacturing, Private Bag 10, Clayton, Melbourne, VIC 3169, Australia. zhuyonggang@hitsz.edu.cn.
authors.forename: M
authors.initials: M
authors.lastname: Best
authors.affiliation: RMIT University, Melbourne, VIC 3001, Australia.
authors.forename: D
authors.initials: D
authors.lastname: Lester
authors.affiliation: CSIRO Manufacturing, Private Bag 10, Clayton, Melbourne, VIC 3169, Australia. zhuyonggang@hitsz.edu.cn and Harbin Institute of technology (Shenzhen), Shenzhen, Guangdong 518055, China and RMIT University, Melbourne, VIC 3001, Australia.
authors.forename: Y
authors.identifier: 0000-0002-5419-9508
authors.initials: Y
authors.lastname: Zhu
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D045404:Hendra Virus / Q000276:immunology*; D045464:Henipavirus Infections / Q000175:diagnosis / Q000662:veterinary / Q000821:virology; D006736:Horses; D007118:Immunoassay / Q000295:instrumentation / Q000379:methods*; D056656:Lab-On-A-Chip Devices; D058185:Magnetite Nanoparticles / Q000737:chemistry*
chemical_list: D000914:Antibodies, Viral; D058185:Magnetite Nanoparticles