title: Phylogenetic background of enterotoxigenic and enteroinvasiveEscherichia colifrom patients with diarrhea in Sirjan, Iran.
abstract: OBJECTIVE
  DiarrheagenicEscherichia coli(DEC) strains are a major cause of intestinal syndromes in the developing countries. The aim of this study was to determine the prevalence of enterotoxigenicE. coli(ETEC) and enteroinvasiveE. coli(EIEC) in relation to phylogenetic background from patients with diarrhea.
  
  
  METHODS
  A total of 110E. coliisolates were obtained from diarrhea patients in Sirjan, southeast of Iran. TheE. coliisolates were confirmed using biochemical and bacteriological tests. DNA ofE. coliisolates was extracted by boiling method and checked for existence of ETEC (LTandSTgenes) and EIEC (ipaHgene) pathotypes and also characterize the phylogenetic groups on the basis of presence or absence of thechuA, yjaAgenes and an anonymous DNA fragment, TspE4. C2 by multiplex PCR.
  
  
  RESULTS
  Out of 110E. coliisolates, 32 (29.09%) were positive for ETEC (LTandSTgenes) and 6 (5.45%) possessed EIEC (ipaHgene) pathotypes. Isolates fall into four phylogenetic groups: A (39.09%), B1 (20%), B2 (15.45%) and D (25.45%). Phylotyping of isolates of DEC indicated they were distributed in four phylogenetic groups including A (12 isolates), B1 (7), B2 (9) and D (10).
  
  
  CONCLUSIONS
  In this study, the DEC isolates were segregated into different phylogenetic groups. The majority of isolates belonged to phylo-groups A and D.
authors.affiliation: Department of Biology, Kerman Branch, Islamic Azad University, Kerman, Iran.
authors.forename: Taifeh
authors.initials: T
authors.lastname: Hoseinzadeh
authors.affiliation: Molecular Microbiology Department, Faculty of Veterinary Medicine, Shahid Bahonar University, Kerman, Iran.
authors.forename: Reza
authors.initials: R
authors.lastname: Ghanbarpour
authors.affiliation: Department of Biology, Kerman Branch, Islamic Azad University, Kerman, Iran.
authors.forename: Farokh
authors.initials: F
authors.lastname: Rokhbakhsh-Zamin
keywords: Diarrhea; Diarrheagenic Escherichia coli; Phylogenetic group