title: NFκB-Induced Periostin Activates Integrin-β3 Signaling to Promote Renal Injury in GN.
abstract: De novoexpression in the kidney of periostin, a protein involved in odontogenesis and osteogenesis, has been suggested as a biomarker of renal disease. In this study, we investigated the mechanism(s) of induction and the role of periostin in renal disease. Using a combination of bioinformatics, reporter assay, and chromatin immunoprecipitation analyses, we found that NFκB and other proinflammatory transcription factors induce periostin expressionin vitroand that binding of these factors on the periostin promoter is enriched in glomeruli during experimental GN. Mice lacking expression of periostin displayed preserved renal function and structure during GN. Furthermore, delayed administration of periostin antisense oligonucleotides in wild-type animals with GN reversed already established proteinuria, diminished tissue inflammation, and improved renal structure. Lack of periostin expression also blunted thede novorenal expression of integrin-β3 and phosphorylation of focal adhesion kinase and AKT, known mediators of integrin-β3 signaling that affect cell motility and survival, observed during GN in wild-type animals.In vitro, recombinant periostin increased the expression of integrin-β3 and the concomitant phosphorylation of focal adhesion kinase and AKT in podocytes. Notably, periostin and integrin-β3 were highly colocalized in biopsy specimens from patients with inflammatory GN. These results demonstrate that interplay between periostin and renal inflammation orchestrates inflammatory and fibrotic responses, driving podocyte damage through downstream activation of integrin-β3 signaling. Targeting periostin may be a novel therapeutic strategy for treating CKD.
authors.affiliation: Institut National de la Santé Et de la Recherche Médicale, Unité Mixte de Recherche Scientifique 1155, Tenon Hospital, Paris, France.
authors.forename: Niki
authors.initials: N
authors.lastname: Prakoura
authors.affiliation: Institut National de la Santé Et de la Recherche Médicale, Unité Mixte de Recherche Scientifique 1155, Tenon Hospital, Paris, France.
authors.forename: Panagiotis
authors.initials: P
authors.lastname: Kavvadas
authors.affiliation: Institut National de la Santé Et de la Recherche Médicale, Unité Mixte de Recherche Scientifique 1155, Tenon Hospital, Paris, France.|Sorbonne Université, Université Pierre-et-Marie-Curie Paris 6, Paris, France; and.
authors.forename: Raphaёl
authors.initials: R
authors.lastname: Kormann
authors.affiliation: Institut National de la Santé Et de la Recherche Médicale, Unité Mixte de Recherche Scientifique 1155, Tenon Hospital, Paris, France.|Sorbonne Université, Université Pierre-et-Marie-Curie Paris 6, Paris, France; and.|Department of Physiology, Saint-Antoine Hospital, Assistance Publique-Hôpitaux de Paris, Paris, France.
authors.forename: Jean-Claude
authors.initials: JC
authors.lastname: Dussaule
authors.affiliation: Institut National de la Santé Et de la Recherche Médicale, Unité Mixte de Recherche Scientifique 1155, Tenon Hospital, Paris, France.|Sorbonne Université, Université Pierre-et-Marie-Curie Paris 6, Paris, France; and.
authors.forename: Christos E
authors.initials: CE
authors.lastname: Chadjichristos
authors.affiliation: Institut National de la Santé Et de la Recherche Médicale, Unité Mixte de Recherche Scientifique 1155, Tenon Hospital, Paris, France; christos.chatziantoniou@upmc.fr.|Sorbonne Université, Université Pierre-et-Marie-Curie Paris 6, Paris, France; and.
authors.forename: Christos
authors.initials: C
authors.lastname: Chatziantoniou
mesh_terms: D000818:Animals; D015815:Cell Adhesion Molecules / Q000502:physiology*; D005260:Female; D005921:Glomerulonephritis / Q000150:complications; D039661:Integrin beta3 / Q000502:physiology*; D007674:Kidney Diseases / Q000209:etiology* / Q000473:pathology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D016328:NF-kappa B / Q000502:physiology*; D015398:Signal Transduction
chemical_list: D015815:Cell Adhesion Molecules; D039661:Integrin beta3; D016328:NF-kappa B; C082838:Postn protein, mouse
keywords: Pathophysiology of Renal Disease and Progression; cell-matrix interactions; glomerulonephritis; macrophages; transcription regulation