title: Global DNA methylation variations after short-term heat shock treatment in cultured microspores of Brassica napus cv. Topas.
abstract: Heat stress can induce the cultured microspores into embryogenesis. In this study, whole genome bisulphite sequencing was employed to study global DNA methylation variations after short-term heat shock (STHS) treatments in cultured microspores of Brassica napus cv. Topas. Our results indicated that treatment on cultured Topas microspores at 32 °C for 6 h triggered DNA hypomethylation, particularly in the CG and CHG contexts. And the total number of T32 (Topas 32 °C for 6 h) vs. T0 (Topas 0 h) differentially methylated region-related genes (DRGs) was approximately two-fold higher than that of T18 (Topas 18 °C for 6 h) vs. T0 DRGs, which suggested that 32 °C might be a more intense external stimulus than 18 °C resulting in more changes in the DNA methylation status of cultured microspores. Additionally, 32 °C treatment for 6 h led to increased CHG differential methylations of transposons (DMTs), which were mainly constituted by overlaps between the hypomethylated differentially methylated regions (hypo-DMRs) and transposon elements (TEs). Further analysis demonstrated that the DRGs and their paralogs exhibited differential methylated/demethylated patterns. To summarize, the present study is the first methylome analysis of cultured microspores in response to STHS and may provide valuable information on the roles of DNA methylation in heat response.
authors.affiliation: Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture, Wuhan 430062, P. R. China.
authors.forename: Jun
authors.initials: J
authors.lastname: Li
authors.affiliation: Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture, Wuhan 430062, P. R. China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Huang
authors.affiliation: Department of Cell and Developmental Biology, College of Life Science, State Key Laboratory of Hybrid Rice, Wuhan University, Wuhan 430072, China.
authors.forename: Mengxiang
authors.initials: M
authors.lastname: Sun
authors.affiliation: Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture, Wuhan 430062, P. R. China.
authors.forename: Tianyao
authors.initials: T
authors.lastname: Zhang
authors.affiliation: Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture, Wuhan 430062, P. R. China.
authors.forename: Hao
authors.initials: H
authors.lastname: Li
authors.affiliation: Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture, Wuhan 430062, P. R. China.
authors.forename: Biyun
authors.initials: B
authors.lastname: Chen
authors.affiliation: Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture, Wuhan 430062, P. R. China.
authors.forename: Kun
authors.initials: K
authors.lastname: Xu
authors.affiliation: Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture, Wuhan 430062, P. R. China.
authors.forename: Guizhen
authors.initials: G
authors.lastname: Gao
authors.affiliation: Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture, Wuhan 430062, P. R. China.
authors.forename: Feng
authors.initials: F
authors.lastname: Li
authors.affiliation: Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture, Wuhan 430062, P. R. China.
authors.forename: Guixin
authors.initials: G
authors.lastname: Yan
authors.affiliation: Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture, Wuhan 430062, P. R. China.
authors.forename: Jiangwei
authors.initials: J
authors.lastname: Qiao
authors.affiliation: Shanghai Greenglobe Biotechnology Co., Ltd, Shanghai 201499, P. R. China.
authors.forename: Yongping
authors.initials: Y
authors.lastname: Cai
authors.affiliation: Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture, Wuhan 430062, P. R. China.
authors.forename: Xiaoming
authors.initials: X
authors.lastname: Wu
mesh_terms: D029688:Brassica napus / Q000235:genetics* / Q000378:metabolism; D002874:Chromosome Mapping; D032461:Chromosomes, Plant / Q000737:chemistry / Q000378:metabolism; D019175:DNA Methylation; D004251:DNA Transposable Elements; D018506:Gene Expression Regulation, Plant; D063990:Gene Ontology; D056426:Genetic Loci; D018869:Heat-Shock Response / Q000235:genetics; D006358:Hot Temperature; D058977:Molecular Sequence Annotation; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D011058:Pollen / Q000235:genetics* / Q000378:metabolism
chemical_list: D004251:DNA Transposable Elements; D010940:Plant Proteins