title: Transcriptome analysis of resistant and susceptible tobacco (Nicotiana tabacum) in response to root-knot nematode Meloidogyne incognita infection.
abstract: The root-knot nematode (RKN) Meloidogyne incognita reproduces on the roots of tobacco (Nicotiana tabacum), damaging crops, reducing crop yield, and causing economic losses annually. The development of resistant genotypes is an alternative strategy to effectively control these losses. However, the molecular mechanism responsible for host pathogenesis and defense responses in tobacco specifically against RKNs remain poorly understood. Here, root transcriptome analysis of resistant (Yuyan12) and susceptible (Changbohuang) tobacco varieties infected with RKNs was performed. Moreover, 2623 and 545 differentially expressed genes (DEGs) in RKN-infected roots were observed in Yuyan12 and Changbohuang, respectively, compared to those in non-infected roots, including 289 DEGs commonly expressed in the two genotypes. Among these DEGs, genes encoding cell wall modifying proteins, auxin-related proteins, the ROS scavenging system, and transcription factors involved in various biological and physiochemical processes were significantly expressed in both the resistant and susceptible genotypes. This work is thus the first report on the relationships in the RKN-tobacco interaction using transcriptome analysis, and the results provide important information on the mechanism of RKN resistance in tobacco.
authors.affiliation: College of Tobacco, Henan Agricultural University, Zhengzhou 450002, Henan, China.
authors.forename: Xuexia
authors.initials: X
authors.lastname: Xing
authors.affiliation: College of Tobacco, Henan Agricultural University, Zhengzhou 450002, Henan, China.
authors.forename: Xiaohui
authors.initials: X
authors.lastname: Li
authors.affiliation: College of Tobacco, Henan Agricultural University, Zhengzhou 450002, Henan, China.
authors.forename: Mingzhen
authors.initials: M
authors.lastname: Zhang
authors.affiliation: College of Tobacco, Henan Agricultural University, Zhengzhou 450002, Henan, China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Wang
authors.affiliation: College of Tobacco, Henan Agricultural University, Zhengzhou 450002, Henan, China.
authors.forename: Bingyang
authors.initials: B
authors.lastname: Liu
authors.affiliation: College of Tobacco, Henan Agricultural University, Zhengzhou 450002, Henan, China.
authors.forename: Qiliang
authors.initials: Q
authors.lastname: Xi
authors.affiliation: College of Tobacco, Henan Agricultural University, Zhengzhou 450002, Henan, China.
authors.forename: Ke
authors.initials: K
authors.lastname: Zhao
authors.affiliation: College of Tobacco, Henan Agricultural University, Zhengzhou 450002, Henan, China.
authors.forename: Yunjie
authors.initials: Y
authors.lastname: Wu
authors.affiliation: College of Tobacco, Henan Agricultural University, Zhengzhou 450002, Henan, China. Electronic address: yangtiezhao0102@126.com.
authors.forename: Tiezhao
authors.initials: T
authors.lastname: Yang
mesh_terms: D000818:Animals; D018533:Biomass; D002874:Chromosome Mapping; D016000:Cluster Analysis; D019295:Computational Biology; D018556:Crops, Agricultural / Q000378:metabolism / Q000469:parasitology; D060467:Disease Resistance; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D005838:Genotype; D010935:Plant Diseases / Q000469:parasitology; D018517:Plant Roots / Q000378:metabolism / Q000469:parasitology; D017382:Reactive Oxygen Species / Q000378:metabolism; D017423:Sequence Analysis, RNA; D014026:Nicotiana / Q000378:metabolism* / Q000469:parasitology*; D059467:Transcriptome; D014431:Tylenchoidea / Q000472:pathogenicity*
chemical_list: D017382:Reactive Oxygen Species
keywords: Resistance; Root-knot nematode; Tobacco; Transcriptome