title: Adhesion and invasion of Streptococcus pneumoniae to primary and secondary respiratory epithelial cells.
abstract: The interaction between Streptococcus pneumoniae (S. pneumoniae) and the mucosal epithelial cells of its host is a prerequisite for pneumococcal disease development, yet the specificity of this interaction between different respiratory cells is not fully understood. In the present study, three areas were examined: i) The capability of the encapsulated S. pneumoniae serotype 3 strain (WU2) to adhere to and invade primary nasal‑derived epithelial cells in comparison to primary oral‑derived epithelial cells, A549 adenocarcinoma cells and BEAS‑2B viral transformed bronchial cells; ii) the capability of the unencapsulated 3.8DW strain (a WU2 derivative) to adhere to and invade the same cells over time; and iii) the ability of various genetically‑unrelated encapsulated and unencapsulated S. pneumoniae strains to adhere to and invade A549 lung epithelial cells. The results of the present study demonstrated that the encapsulated WU2 strain adhesion to and invasion of primary nasal epithelial cells was greatest, followed by BEAS‑2B, A549 and primary oral epithelial cells. By contrast, the unencapsulated 3.8‑DW strain invaded oral epithelial cells significantly more efficiently when compared to the nasal epithelial cells. In addition, unencapsulated S. pneumoniae strains adhered to and invaded the A459 cells significantly more efficiently than the encapsulated strains; this is consistent with previously published data. In conclusion, the findings presented in the current study indicated that the adhesion and invasion of the WU2 strain to primary nasal epithelial cells was more efficient compared with the other cultured respiratory epithelial cells tested, which corresponds to the natural course of S. pneumoniae infection and disease development. The target cell preference of unencapsulated strains was different from that of the encapsulated strains, which may be due to the exposure of cell wall proteins.
authors.affiliation: Shraga Segal Department of Microbiology and Immunology, Faculty of Health Sciences, Ben‑Gurion University of The Negev, Beer Sheva 84101, Israel.
authors.forename: Sara
authors.initials: S
authors.lastname: Novick
authors.affiliation: Shraga Segal Department of Microbiology and Immunology, Faculty of Health Sciences, Ben‑Gurion University of The Negev, Beer Sheva 84101, Israel.
authors.forename: Marilous
authors.initials: M
authors.lastname: Shagan
authors.affiliation: Shraga Segal Department of Microbiology and Immunology, Faculty of Health Sciences, Ben‑Gurion University of The Negev, Beer Sheva 84101, Israel.
authors.forename: Karin
authors.initials: K
authors.lastname: Blau
authors.affiliation: Shraga Segal Department of Microbiology and Immunology, Faculty of Health Sciences, Ben‑Gurion University of The Negev, Beer Sheva 84101, Israel.
authors.forename: Sarit
authors.initials: S
authors.lastname: Lifshitz
authors.affiliation: Pediatric Infectious Disease Unit, Soroka University Medical Center, Faculty of Health Sciences, Ben‑Gurion University of The Negev, Beer Sheva 84101, Israel.
authors.forename: Noga
authors.initials: N
authors.lastname: Givon-Lavi
authors.affiliation: Shraga Segal Department of Microbiology and Immunology, Faculty of Health Sciences, Ben‑Gurion University of The Negev, Beer Sheva 84101, Israel.
authors.forename: Nili
authors.initials: N
authors.lastname: Grossman
authors.affiliation: Oral and Maxillofacial Surgery Unit, Soroka University Medical Center, Beer Sheva 84105, Israel.
authors.forename: Lipa
authors.initials: L
authors.lastname: Bodner
authors.affiliation: Faculty of Health Sciences, Ben‑Gurion University of The Negev, Beer Sheva 84101, Israel.
authors.forename: Ron
authors.initials: R
authors.lastname: Dagan
authors.affiliation: Shraga Segal Department of Microbiology and Immunology, Faculty of Health Sciences, Ben‑Gurion University of The Negev, Beer Sheva 84101, Israel.
authors.forename: Yaffa
authors.initials: Y
authors.lastname: Mizrachi Nebenzahl
mesh_terms: D001422:Bacterial Adhesion; D002460:Cell Line; D002478:Cells, Cultured; D004847:Epithelial Cells / Q000382:microbiology / Q000473:pathology; D006801:Humans; D009061:Mouth Mucosa / Q000166:cytology / Q000382:microbiology / Q000473:pathology; D009297:Nasal Mucosa / Q000166:cytology / Q000382:microbiology / Q000473:pathology; D011008:Pneumococcal Infections / Q000382:microbiology / Q000473:pathology*; D020545:Respiratory Mucosa / Q000382:microbiology* / Q000473:pathology*; D012141:Respiratory Tract Infections / Q000382:microbiology / Q000473:pathology; D013296:Streptococcus pneumoniae / Q000502:physiology*