title: The effect of magnetic nanoparticles on neuronal differentiation of induced pluripotent stem cell-derived neural precursors.
abstract: BACKGROUND
  Magnetic resonance (MR) imaging is suitable for noninvasive long-term tracking. We labeled human induced pluripotent stem cell-derived neural precursors (iPSC-NPs) with two types of iron-based nanoparticles, silica-coated cobalt zinc ferrite nanoparticles (CZF) and poly-l-lysine-coated iron oxide superparamagnetic nanoparticles (PLL-coated γ-Fe2O3) and studied their effect on proliferation and neuronal differentiation.
  
  
  METHODS
  We investigated the effect of these two contrast agents on neural precursor cell proliferation and differentiation capability. We further defined the intracellular localization and labeling efficiency and analyzed labeled cells by MR.
  
  
  RESULTS
  Cell proliferation was not affected by PLL-coated γ-Fe2O3but was slowed down in cells labeled with CZF. Labeling efficiency, iron content and relaxation rates measured by MR were lower in cells labeled with CZF when compared to PLL-coated γ-Fe2O3. Cytoplasmic localization of both types of nanoparticles was confirmed by transmission electron microscopy. Flow cytometry and immunocytochemical analysis of specific markers expressed during neuronal differentiation did not show any significant differences between unlabeled cells or cells labeled with both magnetic nanoparticles.
  
  
  CONCLUSIONS
  Our results show that cells labeled with PLL-coated γ-Fe2O3are suitable for MR detection, did not affect the differentiation potential of iPSC-NPs and are suitable for in vivo cell therapies in experimental models of central nervous system disorders.
authors.affiliation: Department of Neuroscience, Institute of Experimental Medicine, Academy of Sciences of the Czech Republic.
authors.forename: Klára
authors.initials: K
authors.lastname: Jiráková
authors.affiliation: Department of Neuroscience, Institute of Experimental Medicine, Academy of Sciences of the Czech Republic; Department of Neuroscience, Second Faculty of Medicine, Charles University.
authors.forename: Monika
authors.initials: M
authors.lastname: Šeneklová
authors.affiliation: MR-Unit, Radiodiagnostic and Interventional Radiology Department, Institute for Clinical and Experimental Medicine; Department of Biophysics, Institute of Biophysics and Informatics, First Faculty of Medicine, Charles University.
authors.forename: Daniel
authors.initials: D
authors.lastname: Jirák
authors.affiliation: Department of Neuroscience, Institute of Experimental Medicine, Academy of Sciences of the Czech Republic.
authors.forename: Karolína
authors.initials: K
authors.lastname: Turnovcová
authors.affiliation: Department of Analytical Chemistry, University of Chemistry and Technology.
authors.forename: Magda
authors.initials: M
authors.lastname: Vosmanská
authors.affiliation: Department of Polymer Particles, Institute of Macromolecular Chemistry.
authors.forename: Michal
authors.initials: M
authors.lastname: Babič
authors.affiliation: Department of Polymer Particles, Institute of Macromolecular Chemistry.
authors.forename: Daniel
authors.initials: D
authors.lastname: Horák
authors.affiliation: Department of Magnetics and Superconductors, Institute of Physics, ASCR, Prague, Czech Republic.
authors.forename: Pavel
authors.initials: P
authors.lastname: Veverka
authors.affiliation: Department of Neuroscience, Institute of Experimental Medicine, Academy of Sciences of the Czech Republic; Department of Neuroscience, Second Faculty of Medicine, Charles University.
authors.forename: Pavla
authors.initials: P
authors.lastname: Jendelová
mesh_terms: D002454:Cell Differentiation; D049109:Cell Proliferation; D002478:Cells, Cultured; D003287:Contrast Media / Q000737:chemistry; D005260:Female; D005333:Fetus / Q000166:cytology*; D005347:Fibroblasts / Q000166:cytology*; D005434:Flow Cytometry; D006801:Humans; D007124:Immunoenzyme Techniques; D057026:Induced Pluripotent Stem Cells / Q000166:cytology*; D008168:Lung / Q000166:cytology*; D008239:Lysine / Q000737:chemistry; D008279:Magnetic Resonance Imaging / Q000379:methods; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D046529:Microscopy, Electron, Transmission; D009474:Neurons / Q000166:cytology*; D060888:Real-Time Polymerase Chain Reaction
chemical_list: D003287:Contrast Media; D058185:Magnetite Nanoparticles; D008239:Lysine
keywords: cell differentiation; ferrites; magnetic resonance imaging; neural precursors; superparamagnetic iron oxide nanoparticles