title: Water influx via aquaporin directly determines necrotic cell death induced by the Bacillus thuringiensis Cry toxin.
abstract: The Bacillus thuringiensis Cry toxin causes swelling and necrosis in insect cells, but the route(s) and significance of the water influx involved in its cytotoxicity are unclear. Here, we assessed the role of aquaporins (AQPs), known as water channels, in Cry toxin intoxication. An AQP inhibitor did not interfere with any known process to form the toxin pore, but it diminished the cell swelling and loss of membrane integrity induced by the Cry toxin. Overexpression of AQPs facilitated water influx and cytotoxicity. Our results demonstrate that water influx via aquaporin directly determines necrotic cell death induced by the Cry toxin.
authors.affiliation: Graduate School of Bio-Applications and Systems Engineering, Tokyo University of Agriculture and Technology, Koganei, Japan.|Research Fellow of Japan Society for the Promotion of Science, Tokyo, Japan.
authors.forename: Haruka
authors.initials: H
authors.lastname: Endo
authors.affiliation: Faculty of Agriculture, Tottori University, Japan.
authors.forename: Masaaki
authors.initials: M
authors.lastname: Azuma
authors.affiliation: Graduate School of Bio-Applications and Systems Engineering, Tokyo University of Agriculture and Technology, Koganei, Japan.
authors.forename: Satomi
authors.initials: S
authors.lastname: Adegawa
authors.affiliation: Graduate School of Bio-Applications and Systems Engineering, Tokyo University of Agriculture and Technology, Koganei, Japan.
authors.forename: Shingo
authors.initials: S
authors.lastname: Kikuta
authors.affiliation: Graduate School of Bio-Applications and Systems Engineering, Tokyo University of Agriculture and Technology, Koganei, Japan.
authors.forename: Ryoichi
authors.initials: R
authors.lastname: Sato
mesh_terms: D000818:Animals; D020346:Aquaporins / Q000378:metabolism*; D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins / Q000494:pharmacology*; D001692:Biological Transport / Q000187:drug effects; D016923:Cell Death / Q000187:drug effects; D048429:Cell Size / Q000187:drug effects; D004731:Endotoxins / Q000494:pharmacology*; D006460:Hemolysin Proteins / Q000494:pharmacology*; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D010802:Phylogeny; D011485:Protein Binding / Q000187:drug effects; D061987:Sf9 Cells; D012964:Sodium / Q000378:metabolism; D013451:Sulfonic Acids / Q000494:pharmacology; D013997:Time Factors; D014867:Water / Q000378:metabolism*
chemical_list: D020346:Aquaporins; D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins; D004731:Endotoxins; D006460:Hemolysin Proteins; D013451:Sulfonic Acids; C030956:insecticidal crystal protein, Bacillus Thuringiensis; D014867:Water; D012964:Sodium; D007770:L-Lactate Dehydrogenase
keywords: 
  Bacillus thuringiensis
  ; Cry toxin; aquaporin; cell death; cell volume; osmotic swelling