title: Gene Transfer in Isolated Adult Cardiomyocytes.
abstract: During the past few decades, gene delivery using recombinant virus has made tremendous progress. With a higher than 80 % transduction efficiency, even in non-dividing cells, viral transduction has become the method of choice for efficient gene transfer into cardiomyocytes. However, in vitro gene delivery is dependent on a robust cell isolation protocol, as prolonged cultivation is needed to initiate gene expression and target specific cellular processes. This chapter describes some of the important steps that need to be considered for successful in vitro gene transfer into adult cardiomyocytes. Included are detailed protocols for isolating cells, maintaining rod shaped cardiomyocytes in culture over several days, and employing adenovirus for gene transduction.
authors.affiliation: Department of Basic Sciences and Aquatic Medicine, Norwegian University of Life Sciences (NMBU), Oslo, Norway.
authors.forename: Kjetil
authors.initials: K
authors.lastname: Hodne
authors.affiliation: Institute for Experimental Medical Research, Oslo University Hospital, University of Oslo, Ullevål, Kirkeveien 166, 0407, Oslo, Norway.|KG Jebsen Cardiac Research Center and Center for Heart Failure Research, University of Oslo, Oslo, Norway.
authors.forename: David B
authors.initials: DB
authors.lastname: Lipsett
authors.affiliation: Institute for Experimental Medical Research, Oslo University Hospital, University of Oslo, Ullevål, Kirkeveien 166, 0407, Oslo, Norway. w.e.louch@medisin.uio.no.|KG Jebsen Cardiac Research Center and Center for Heart Failure Research, University of Oslo, Oslo, Norway. w.e.louch@medisin.uio.no.
authors.forename: William E
authors.initials: WE
authors.lastname: Louch
mesh_terms: D000375:Aging / Q000502:physiology*; D000818:Animals; D002469:Cell Separation / Q000379:methods*; D002478:Cells, Cultured; D018014:Gene Transfer Techniques; D032383:Myocytes, Cardiac / Q000378:metabolism*; D051381:Rats; D012996:Solutions
chemical_list: D012996:Solutions
keywords: Cardiomyocyte; Cell culture; Cell isolation; Gene transfer; Transduction; Virus