title: Heteroglycan of an edible mushroom Pleurotus cystidiosus: Structural characterization and study of biological activities.
abstract: A water soluble heteroglycan (PCPS) was isolated from the aqueous extract of an edible mushroom Pleurotus cystidiosus. Structural characterization of the heteroglycan was carried out using total hydrolysis, methylation analysis, periodate oxidation, Smith degradation, and 1D/2D NMR experiments. Sugar analysis indicated the presence of glucose, galactose, and mannose in a molar ratio of nearly 6:2:1 respectively. The chemical and NMR analysis of the PCPS indicated the presence of a repeating unit with a backbone consisting of one unit of (1→6)-β-d-glucopyranosyl, two (1→3)-β-d-glucopyranosyl, one (1→3)-α-d-glucopyranosyl, one (1→6)-α-d-glucopyranosyl, and two (1→6)-α-d-galactopyranosyl moieties respectively, out of which one (1→3)-β-d-glucopyranosyl residue was branched at O-6 with terminal β-d-glucopyranosyl and another (1→6)-α-d-galactopyranosyl residue was branched at O-2 with terminal β-d-mannopyranosyl moiety. The polysaccharide was found to exhibit cellular activities at different concentrations (10, 25, 50, 100, 200, 400μg/mL) and maintained the redox balance as well as reduced lipid per oxidation which protect the cell destruction.
authors.affiliation: Department of Chemistry and Chemical Technology, Vidyasagar University, Midnapore, 721102, West Bengal, India.
authors.forename: Bibhash C
authors.initials: BC
authors.lastname: Panda
authors.affiliation: Department of Chemistry and Chemical Technology, Vidyasagar University, Midnapore, 721102, West Bengal, India.
authors.forename: Prasenjit
authors.initials: P
authors.lastname: Maity
authors.affiliation: Department of Chemistry and Chemical Technology, Vidyasagar University, Midnapore, 721102, West Bengal, India.
authors.forename: Ashis K
authors.initials: AK
authors.lastname: Nandi
authors.affiliation: Department of Chemistry and Chemical Technology, Vidyasagar University, Midnapore, 721102, West Bengal, India.
authors.forename: Manabendra
authors.initials: M
authors.lastname: Pattanayak
authors.affiliation: Department of Chemistry and Chemical Technology, Vidyasagar University, Midnapore, 721102, West Bengal, India.
authors.forename: Dilip K
authors.initials: DK
authors.lastname: Manna
authors.affiliation: Department of Chemistry, Panskura Banamali College, Purba Medinipur, 721152, West Bengal, India.
authors.forename: Soumitra
authors.initials: S
authors.lastname: Mondal
authors.affiliation: Immunology and Microbiology Laboratory, Department of Human Physiology with Community Health, Vidyasagar University, Midnapore, 721 102, West Bengal, India.
authors.forename: Satyajit
authors.initials: S
authors.lastname: Tripathy
authors.affiliation: Immunology and Microbiology Laboratory, Department of Human Physiology with Community Health, Vidyasagar University, Midnapore, 721 102, West Bengal, India.
authors.forename: Somenath
authors.initials: S
authors.lastname: Roy
authors.affiliation: Molecular and Applied Mycology and Plant Pathology Laboratory, Department of Botany, University of Calcutta, 35, Ballygunge Circular Road, Kolkata, 700019, West Bengal, India.
authors.forename: Krishnendu
authors.initials: K
authors.lastname: Acharya
authors.affiliation: Department of Chemistry and Chemical Technology, Vidyasagar University, Midnapore, 721102, West Bengal, India. Electronic address: sirajul_1999@yahoo.com.
authors.forename: Syed S
authors.initials: SS
authors.lastname: Islam
mesh_terms: D000975:Antioxidants / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology* / Q000633:toxicity; D002240:Carbohydrate Sequence; D004305:Dose-Response Relationship, Drug; D006801:Humans; D008214:Lymphocytes / Q000166:cytology / Q000187:drug effects; D020076:Pleurotus / Q000737:chemistry*; D011134:Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology* / Q000633:toxicity; D014867:Water / Q000737:chemistry
chemical_list: D000975:Antioxidants; D011134:Polysaccharides; D014867:Water
keywords: Biological activities; NMR studies; Pleurotus cystidiosus