title: Sinu virus, a novel and divergent orthomyxovirus related to members of the genus Thogotovirus isolated from mosquitoes in Colombia.
abstract: The genome and structural organization of a novel insect-specific orthomyxovirus, designated Sinu virus, is described. Sinu virus (SINUV) was isolated in cultures of C6/36 cells from a pool of mosquitoes collected in northwestern Colombia. The virus has six negative-sense ssRNA segments. Genetic analysis of each segment demonstrated the presence of six distinct ORFs encoding the following genes: PB2 (Segment 1), PB1, (Segment 2), PA protein (Segment 3), envelope GP gene (Segment 4), the NP (Segment 5), and M-like gene (Segment 6). Phylogenetically, SINUV appears to be most closed related to viruses in the genus Thogotovirus.
authors.affiliation: Programa de Estudio y Control de Enfermedades Tropicales - PECET - SIUSde de Investigación Universitaria - Universidad de Antioquia, Medellín, Colombia; Grupo de Investigación en Sistemática Molecular-GSM, Facultad de Ciencias, Universidad Nacional de Colombia, sede Medellín, Medellín, Colombia.
authors.forename: María Angélica
authors.initials: MA
authors.lastname: Contreras-Gutiérrez
authors.affiliation: Center for Technological Innovation, Evandro Chagas Institute, Ministry of Health, Ananindeua, Para, Brazil.
authors.forename: Marcio R T
authors.initials: MRT
authors.lastname: Nunes
authors.affiliation: Department of Pathology, Institute for Human Infections and Immunity, University of Texas Medical Branch, Galveston, TX, USA.
authors.forename: Hilda
authors.initials: H
authors.lastname: Guzman
authors.affiliation: Grupo de Investigación en Sistemática Molecular-GSM, Facultad de Ciencias, Universidad Nacional de Colombia, sede Medellín, Medellín, Colombia.
authors.forename: Sandra
authors.initials: S
authors.lastname: Uribe
authors.affiliation: Programa de Estudio y Control de Enfermedades Tropicales - PECET - SIUSde de Investigación Universitaria - Universidad de Antioquia, Medellín, Colombia; Grupo de Investigación en Sistemática Molecular-GSM, Facultad de Ciencias, Universidad Nacional de Colombia, sede Medellín, Medellín, Colombia.
authors.forename: Juan David
authors.initials: JD
authors.lastname: Suaza Vasco
authors.affiliation: Center for Technological Innovation, Evandro Chagas Institute, Ministry of Health, Ananindeua, Para, Brazil.
authors.forename: Jedson F
authors.initials: JF
authors.lastname: Cardoso
authors.affiliation: Department of Pathology, Institute for Human Infections and Immunity, University of Texas Medical Branch, Galveston, TX, USA.
authors.forename: Vsevolod L
authors.initials: VL
authors.lastname: Popov
authors.affiliation: Department of Biochemistry and Molecular Biology, University of Texas Medical Branch, Galveston, TX, USA.
authors.forename: Steven G
authors.initials: SG
authors.lastname: Widen
authors.affiliation: Department of Biochemistry and Molecular Biology, University of Texas Medical Branch, Galveston, TX, USA.
authors.forename: Thomas G
authors.initials: TG
authors.lastname: Wood
authors.affiliation: Department of Pathology, Institute for Human Infections and Immunity, University of Texas Medical Branch, Galveston, TX, USA. Electronic address: nivasila@utmb.edu.
authors.forename: Nikos
authors.initials: N
authors.lastname: Vasilakis
authors.affiliation: Department of Pathology, Institute for Human Infections and Immunity, University of Texas Medical Branch, Galveston, TX, USA. Electronic address: rtesh@utmb.edu.
authors.forename: Robert B
authors.initials: RB
authors.lastname: Tesh
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D003105:Colombia; D009033:Culicidae / Q000821:virology*; D019143:Evolution, Molecular; D016679:Genome, Viral; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009975:Orthomyxoviridae / Q000737:chemistry / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D010802:Phylogeny; D018117:Thogotovirus / Q000737:chemistry / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism
chemical_list: D014764:Viral Proteins
keywords: Insect-specific viruses; Orthomyxoviridae; Sinu virus; Thogotovirus genus