title: Simultaneous Identification and Antimicrobial Susceptibility Testing of Multiple Uropathogens on a Microfluidic Chip with Paper-Supported Cell Culture Arrays.
abstract: A microfluidic chip was developed for one-step identification and antimicrobial susceptibility testing (AST) of multiple uropathogens. The polydimethylsiloxane (PDMS) microchip used had features of cell culture chamber arrays connected through a sample introduction channel. At the bottom of each chamber, a paper substrate preloaded with chromogenic media and antimicrobial agents was embedded. By integrating a hydrophobic membrane valve on the microchip, the urine sample can be equally distributed into and confined in individual chambers. The identification and AST assays on multiple uropathogens were performed by combining the spatial resolution of the cell culture arrays and the color resolution from the chromogenic reaction. The composite microbial testing assay was based on dynamic changes in color in a serial of chambers. The bacterial antimicrobial susceptibility was determined by the lowest concentration of an antimicrobial agent that is capable of inhibiting the chromogenic reaction. Using three common uropathogenic bacteria as test models, the developed microfluidic approach was demonstrated to be able to complete the multiple colorimetric assays in 15 h. The accuracy of the microchip method, in comparison with that of the conventional approach, showed a coincidence of 94.1%. Our data suggest this microfluidic approach will be a promising tool for simple and fast uropathogen testing in resource-limited settings.
authors.affiliation: Department of Laboratory Medicine, Guangzhou First People's Hospital, Affiliated Hospital of Guangzhou Medical University , Guangzhou 510180, China.|Clinical Molecular Medicine and Molecular Diagnosis Key Laboratory of Guangdong Province , Guangzhou 510180, China.
authors.forename: Banglao
authors.initials: B
authors.lastname: Xu
authors.affiliation: Department of Laboratory Medicine, Guangzhou First People's Hospital, Affiliated Hospital of Guangzhou Medical University , Guangzhou 510180, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Du
authors.affiliation: Department of Laboratory Medicine, Guangzhou First People's Hospital, Affiliated Hospital of Guangzhou Medical University , Guangzhou 510180, China.
authors.forename: Jinqiong
authors.initials: J
authors.lastname: Lin
authors.affiliation: Department of Laboratory Medicine, Guangzhou First People's Hospital, Affiliated Hospital of Guangzhou Medical University , Guangzhou 510180, China.
authors.forename: Mingyue
authors.initials: M
authors.lastname: Qi
authors.affiliation: Department of Laboratory Medicine, Guangzhou First People's Hospital, Affiliated Hospital of Guangzhou Medical University , Guangzhou 510180, China.|Clinical Molecular Medicine and Molecular Diagnosis Key Laboratory of Guangdong Province , Guangzhou 510180, China.
authors.forename: Bowen
authors.initials: B
authors.lastname: Shu
authors.affiliation: Department of Laboratory Medicine, Guangzhou First People's Hospital, Affiliated Hospital of Guangzhou Medical University , Guangzhou 510180, China.
authors.forename: Xiaoxia
authors.initials: X
authors.lastname: Wen
authors.affiliation: Department of Laboratory Medicine, Guangzhou First People's Hospital, Affiliated Hospital of Guangzhou Medical University , Guangzhou 510180, China.|Clinical Molecular Medicine and Molecular Diagnosis Key Laboratory of Guangdong Province , Guangzhou 510180, China.
authors.forename: Guangtie
authors.initials: G
authors.lastname: Liang
authors.affiliation: Department of Laboratory Medicine, Guangzhou First People's Hospital, Affiliated Hospital of Guangzhou Medical University , Guangzhou 510180, China.|Clinical Molecular Medicine and Molecular Diagnosis Key Laboratory of Guangdong Province , Guangzhou 510180, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Chen
authors.affiliation: Department of Laboratory Medicine, Guangzhou First People's Hospital, Affiliated Hospital of Guangzhou Medical University , Guangzhou 510180, China.|Clinical Molecular Medicine and Molecular Diagnosis Key Laboratory of Guangdong Province , Guangzhou 510180, China.
authors.forename: Dayu
authors.initials: D
authors.lastname: Liu
mesh_terms: D000900:Anti-Bacterial Agents / Q000032:analysis* / Q000494:pharmacology; D018929:Cell Culture Techniques; D004129:Dimethylpolysiloxanes; D013293:Enterococcus faecalis / Q000166:cytology / Q000187:drug effects; D004926:Escherichia coli / Q000166:cytology / Q000187:drug effects; D006801:Humans; D008826:Microbial Sensitivity Tests; D046210:Microfluidic Analytical Techniques; D010209:Paper; D013211:Staphylococcus aureus / Q000166:cytology / Q000187:drug effects
chemical_list: D000900:Anti-Bacterial Agents; D004129:Dimethylpolysiloxanes; C013830:baysilon