title: Molecular characterization and phylogeny of Linguatula serrata (Pentastomida: Linguatulidae) based on the nuclear 18S rDNA and mitochondrial cytochrome c oxidase I gene.
abstract: Linguatula serrata, a cosmopolitan parasite, is commonly known as tongue worm belonging to the subclass Pentastomida.We collected the nymphal stage of the worm from mesenteric lymph nodes of cattle and identified these as L. serrata based on morphology and morphometry. The 18S rDNA sequences showed no intraspecific variation, although cox1 sequences showed 99.7-99.9% homology. In the phylogenies inferred from both gene loci, members of the genus Linguatula (order Porocephalida) were closer to those of the order Cephalobaenida than to those of Porocephalida, reflecting a mismatch with the corresponding morphology-based taxonomy. Accordingly, analyses of additional gene loci using a larger number of taxa across the Pentastomida should be undertaken to determine an accurate phylogenetic position within the Arthropoda.
authors.affiliation: Laboratory of Veterinary Parasitology, Faculty of Agriculture, Iwate University, Ueda 3-18-8, Morioka 020-8550, Japan.
authors.forename: Uday Kumar
authors.initials: UK
authors.lastname: Mohanta
authors.forename: Tadashi
authors.initials: T
authors.lastname: Itagaki
mesh_terms: D000818:Animals; D004275:DNA, Ribosomal; D003576:Electron Transport Complex IV / Q000235:genetics*; D050259:Genes, Mitochondrial; D058889:Molecular Typing; D057078:Pentastomida / Q000033:anatomy & histology / Q000145:classification* / Q000201:enzymology / Q000235:genetics; D012337:RNA, Ribosomal, 18S; D017422:Sequence Analysis, DNA
chemical_list: D004275:DNA, Ribosomal; D012337:RNA, Ribosomal, 18S; D003576:Electron Transport Complex IV