title: Effect of metronidazole supplemented with hydroquinone on the adhesion of Lactobacillus acidophilus in ovine vaginal cells.
abstract: This work demonstrates that the addition of metronidazole together with a ubiquitous quinone compound reduces adherence of Lactobacillus acidophilus to ovine vaginal cells. Spectrophotometric and voltammetric studies have shown that neoformed compounds were observed in these systems; there were also changes in their electroactive composition, and the oxidant status had a significantly higher value compared to the control (p<0.05). Based on reduction potential (E; mV), the distribution of electroactive compound concentrations suggests that the compounds with low reduction potential induce this behavior, which would indicate that the addition of metronidazole with a ubiquitous quinone compound to the vaginal system might increase the reductive capacity of these systems. This work shows that the study of behavior and fluctuations of the redox compounds that compose the vaginal environment, in terms of concentration and species of redox molecules, must be hierarchized in order to better understand the early stages of colonization by microorganisms.
authors.affiliation: Laboratorio de Química Biológica I, Facultad de Ciencias Veterinarias, Consejo de Investigaciones, Universidad Nacional de Rosario, Santa Fe, Argentina. Electronic address: tamara_coletti@hotmail.com.
authors.forename: Tamara L
authors.initials: TL
authors.lastname: Coletti Zabala
authors.affiliation: Laboratorio de Química Biológica I, Facultad de Ciencias Veterinarias, Consejo de Investigaciones, Universidad Nacional de Rosario, Santa Fe, Argentina; Cátedra de Microbiología, Facultad de Ciencias Médicas, Universidad Nacional de Rosario, Santa Fe, Argentina.
authors.forename: María E
authors.initials: ME
authors.lastname: Zerbatto
authors.affiliation: Laboratorio de Química Biológica I, Facultad de Ciencias Veterinarias, Consejo de Investigaciones, Universidad Nacional de Rosario, Santa Fe, Argentina.
authors.forename: Elda B R
authors.initials: EB
authors.lastname: Perotti
authors.affiliation: Laboratorio de Química Biológica I, Facultad de Ciencias Veterinarias, Consejo de Investigaciones, Universidad Nacional de Rosario, Santa Fe, Argentina.
authors.forename: Ana M
authors.initials: AM
authors.lastname: Smacchia
authors.affiliation: Cátedra de Microbiología, Facultad de Ciencias Médicas, Universidad Nacional de Rosario, Santa Fe, Argentina.
authors.forename: Adriana
authors.initials: A
authors.lastname: Ombrella
authors.affiliation: Laboratorio de Química Biológica I, Facultad de Ciencias Veterinarias, Consejo de Investigaciones, Universidad Nacional de Rosario, Santa Fe, Argentina.
authors.forename: Alejandro R
authors.initials: AR
authors.lastname: Pidello
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology*; D001422:Bacterial Adhesion / Q000187:drug effects*; D004353:Drug Evaluation, Preclinical; D004357:Drug Synergism; D004563:Electrochemistry; D004847:Epithelial Cells / Q000382:microbiology*; D005260:Female; D006801:Humans; D006873:Hydroquinones / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology*; D007779:Lactobacillus acidophilus / Q000187:drug effects* / Q000502:physiology; D008795:Metronidazole / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology*; D015394:Molecular Structure; D010084:Oxidation-Reduction; D012756:Sheep; D013053:Spectrophotometry; D014621:Vagina / Q000166:cytology / Q000382:microbiology*
chemical_list: D000900:Anti-Bacterial Agents; D006873:Hydroquinones; D008795:Metronidazole; C031927:hydroquinone
keywords: Adhesión microbiana; Células vaginales; Metronidazol; Metronidazole; Microbial adhesion; Ovine; Ovinos; Redox; Vaginal cells; Voltametría; Voltammetry