title: Degradation and half-life of DNA present in biomass from a genetically-modified organism during land application.
abstract: White biotechnology has made a positive impact on the chemical industry by providing safer, more efficient chemical manufacturing processes that have reduced the use of toxic chemicals, harsh reaction conditions, and expensive metal catalysts, which has improved alignment with the principles of Green Chemistry. The genetically-modified (GM) biocatalysts that are utilized in these processes are typically separated from high-value products and then recycled, or eliminated. Elimination routes include disposal in sanitary landfills, incineration, use as a fuel, animal feed, or reuse as an agricultural soil amendment or other value-added products. Elimination routes that have the potential to impact the food chain or environment have been more heavily scrutinized for the fate and persistence of biological products. In this study, we developed and optimized a method for monitoring the degradation of strain-specific DNA markers from a genetically-modified organism (GMO) used for the commercial production of 1,3-propanediol. Laboratory and field tests showed that a marker for heterologous DNA in the GM organism was no longer detectable by end-point polymerase chain reaction (PCR) after 14 days. The half-life of heterologous DNA was increased by 17% (from 42.4 to 49.7 h) after sterilization of the soil from a field plot, which indicated that abiotic factors were important in degradation of DNA under field conditions. There was no evidence for horizontal transfer of DNA target sequences from the GMO to viable organisms present in the soil.
authors.affiliation: DuPont Tate and Lyle Bio Products, 198 Blair Bend Drive, Loudon, TN, 37774, USA.
authors.forename: Mathew C
authors.initials: MC
authors.lastname: Halter
authors.affiliation: DuPont Tate and Lyle Bio Products, 198 Blair Bend Drive, Loudon, TN, 37774, USA. james.a.zahn@dupont.com.
authors.forename: James A
authors.initials: JA
authors.lastname: Zahn
mesh_terms: D018533:Biomass; D056915:DNA Copy Number Variations; D004269:DNA, Bacterial / Q000378:metabolism*; D023381:Endpoint Determination; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism; D005285:Fermentation; D005819:Genetic Markers; D006207:Half-Life; D000074041:Microorganisms, Genetically-Modified; D011409:Propylene Glycols / Q000378:metabolism; D017422:Sequence Analysis, DNA; D012987:Soil / Q000737:chemistry; D012988:Soil Microbiology
chemical_list: D004269:DNA, Bacterial; D005819:Genetic Markers; D011409:Propylene Glycols; D012987:Soil; C041787:1,3-propanediol
keywords: 1,3-propanediol; DNA half-life; Fertilizer; GMO; Industrial fermentation; White biotechnology; qPCR