title: Inkjetting Plus Peroxidase-Mediated Hydrogelation Produces Cell-Laden, Cell-Sized Particles with Suitable Characters for Individual Applications.
abstract: The authors report a method to prepare cell-laden, cell-sized microparticles from various materials suitable for individual applications. The method includes a piezoelectric inkjetting technology and a horseradish peroxidase (HRP)-catalyzed crosslinking reaction. The piezoelectric inkjetting technology enables production of cell-laden, cell-sized (20-60 μm) droplets from a polymer aqueous solution. The HRP-catalyzed crosslinking of the polymer in the ejected solution enables production of spherical microparticles from various materials. Superior cytocompatibility of the microencapsulation method is confirmed from the viability and growth profiles of normal murine mammary gland epithelial cells.
authors.affiliation: Department of Materials Science and Engineering, Graduate School of Engineering Science, Osaka University, Osaka, 560-8531, Japan.
authors.forename: Shinji
authors.initials: S
authors.lastname: Sakai
authors.affiliation: Department of Materials Science and Engineering, Graduate School of Engineering Science, Osaka University, Osaka, 560-8531, Japan.
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Yamamoto
authors.affiliation: Department of Materials Science and Engineering, Graduate School of Engineering Science, Osaka University, Osaka, 560-8531, Japan.
authors.forename: Gantumur
authors.initials: G
authors.lastname: Enkhtuul
authors.affiliation: Department of Materials Science and Engineering, Graduate School of Engineering Science, Osaka University, Osaka, 560-8531, Japan.
authors.forename: Kohei
authors.initials: K
authors.lastname: Ueda
authors.affiliation: Department of Regenerative Medicine and Biomedical Engineering, Saga University, Saga, 849-8501, Japan.|Graduate School of Science and Technology for Research, University of Toyama, Toyama, 930-8555, Japan.
authors.forename: Kenichi
authors.initials: K
authors.lastname: Arai
authors.affiliation: Department of Materials Science and Engineering, Graduate School of Engineering Science, Osaka University, Osaka, 560-8531, Japan.
authors.forename: Masahito
authors.initials: M
authors.lastname: Taya
authors.affiliation: Graduate School of Science and Technology for Research, University of Toyama, Toyama, 930-8555, Japan.
authors.forename: Makoto
authors.initials: M
authors.lastname: Nakamura
mesh_terms: D006735:Horseradish Peroxidase / Q000737:chemistry*; D020100:Hydrogels / Q000737:chemistry*; D010316:Particle Size; D011108:Polymers / Q000737:chemistry; D013672:Technology
chemical_list: D020100:Hydrogels; D011108:Polymers; D006735:Horseradish Peroxidase
keywords: cell-laden microparticles; horseradish peroxidase; hydrogels; inkjetting; microencapsulation