title: Molecular cloning and expression analysis of a fish specific interferon regulatory factor, IRF11, in orange spotted grouper, Epinephelus coioides.
abstract: Interferon regulatory factors (IRFs) are transcription mediators which play vital roles in multiple biological processes, such as antiviral defense, immune response, cell growth regulation and apoptosis. A fish specific IRF, termed IRF11, has been identified in previous study through searching fish genome databases. Herein, a transcript of IRF11, EcIRF11 was cloned from orange-spotted grouper, Epinephelus coioides. The EcIRF11 cDNA sequence has 1573 bp in length, encoding a putative protein of 261 amino acids, with a high degree of similarity found between EcIRF11 and its teleost counterparts. Comparative analyses in teleost genomes revealed that IRF11 may have an ancient origin at least 450 million years ago, and the locus harbouring IRF11 might have experienced chromosomal rearrangement and/or inversion during evolution. Expression analysis revealed that the other two members, IRF1 and IRF2 also in the IRF1 subgroup (SG) as IRF11, exhibited high expression levels in early experimental infection phase in response to viral stimulation of poly I:C and to bacterial stimulation of Vibrio parahaemolyticus infections in the fish, while EcIRF11 is not transcriptionally modulated at the examined time points except in kidney at 6 h following poly I:C stimulation. Taken together, the results obtained in this study indicate that IRF11 might have been originated from the same ancestor as IRF1 and IRF2, but exhibits distinct basal and induced expression, implying its different function which needs further characterization.
authors.affiliation: Jimei University, Xiamen, 361021, China; Fujian Collaborative Innovation Center for Development and Utilization of Marine Biological Resources, Xiamen, 361005, China.
authors.forename: Wen Shu
authors.initials: WS
authors.lastname: Huang
authors.affiliation: Jimei University, Xiamen, 361021, China.
authors.forename: Mei Hua
authors.initials: MH
authors.lastname: Zhu
authors.affiliation: Jimei University, Xiamen, 361021, China.
authors.forename: Shan
authors.initials: S
authors.lastname: Chen
authors.affiliation: Jimei University, Xiamen, 361021, China.
authors.forename: Zhi Xuan
authors.initials: ZX
authors.lastname: Wang
authors.affiliation: Jimei University, Xiamen, 361021, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Liang
authors.affiliation: Jimei University, Xiamen, 361021, China; Fujian Collaborative Innovation Center for Development and Utilization of Marine Biological Resources, Xiamen, 361005, China. Electronic address: huangbei@jmu.edu.cn.
authors.forename: Bei
authors.initials: B
authors.lastname: Huang
authors.affiliation: Jimei University, Xiamen, 361021, China. Electronic address: pinnie@ihb.ac.cn.
authors.forename: P
authors.initials: P
authors.lastname: Nie
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001492:Bass; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics / Q000378:metabolism; D005393:Fish Diseases / Q000235:genetics* / Q000276:immunology / Q000382:microbiology; D029941:Fish Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D005786:Gene Expression Regulation; D050835:Interferon Regulatory Factors / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D010802:Phylogeny; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment / Q000662:veterinary; D014735:Vibrio Infections / Q000235:genetics / Q000276:immunology / Q000382:microbiology / Q000662:veterinary*; D014736:Vibrio parahaemolyticus / Q000502:physiology
chemical_list: D018076:DNA, Complementary; D029941:Fish Proteins; D050835:Interferon Regulatory Factors; D012333:RNA, Messenger
keywords: Epinephelus coioides; Expression; IRF11; Orange-spotted grouper