title: Establishment of induced pluripotent stem cell (iPSC) line from an 8-year old female patient with ischemic Moyamoya disease.
abstract: Peripheral blood mononuclear cells (PBMCs) were collected from an 8-year old female patient affected by ischemic Moyamoya disease (MMD). Patient's PBMCs were reprogrammed using Sendai virus particles delivering the four Yamanaka factors. The footprint free hiPSC line expressed the major pluripotency markers and exhibited a normal karyotype. Cells were competent to give rise to progeny of differentiated cells belonging to the 3 germ layers. This hiPSC line represents a good tool to in vitro model MMD in order to shed light on the cellular and molecular mechanisms responsible for the occurrence of this syndrome.
authors.affiliation: Laboratory of Stem Cell Biology, Centre for Integrative Biology-CIBIO, Università degli Studi di Trento, Trento, Italy.
authors.forename: Marina
authors.initials: M
authors.lastname: Cardano
authors.affiliation: Laboratory of Stem Cell Biology, Centre for Integrative Biology-CIBIO, Università degli Studi di Trento, Trento, Italy. Electronic address: fmar@uni-bonn.de.
authors.forename: Fabio
authors.initials: F
authors.lastname: Marsoner
authors.affiliation: Laboratory of Stem Cell Biology, Centre for Integrative Biology-CIBIO, Università degli Studi di Trento, Trento, Italy.
authors.forename: Jacopo
authors.initials: J
authors.lastname: Zasso
authors.affiliation: Laboratory of Stem Cell Biology, Centre for Integrative Biology-CIBIO, Università degli Studi di Trento, Trento, Italy; Department of Health Sciences, Università Degli Studi di Milano, Milan, Italy; Department of Mental Health, San Paolo Hospital, Milan, Italy.
authors.forename: Matteo
authors.initials: M
authors.lastname: Marcatili
authors.affiliation: Chromatin Dynamics Unit, San Raffaele University and Research Institute, Milan, Italy. Electronic address: tk2708@cumc.columbia.edu.
authors.forename: Thodoris
authors.initials: T
authors.lastname: Karnavas
authors.affiliation: Department of Neuroscience and Surgery of the Nervous System, Papa Giovanni XXIII Hospital, Bergamo, Italy. Electronic address: l.lanterna@gmail.com.
authors.forename: Luigi Andrea
authors.initials: LA
authors.lastname: Lanterna
authors.affiliation: Laboratory of Stem Cell Biology, Centre for Integrative Biology-CIBIO, Università degli Studi di Trento, Trento, Italy. Electronic address: Luciano.Conti@unitn.it.
authors.forename: Luciano
authors.initials: L
authors.lastname: Conti
mesh_terms: D002454:Cell Differentiation; D002460:Cell Line; D065150:Cellular Reprogramming; D002648:Child; D058732:Embryoid Bodies / Q000166:cytology / Q000378:metabolism; D005260:Female; D005822:Genetic Vectors / Q000235:genetics / Q000378:metabolism; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000166:cytology* / Q000378:metabolism; D059785:Karyotype; D007963:Leukocytes, Mononuclear / Q000166:cytology; D008856:Microscopy, Fluorescence; D009072:Moyamoya Disease / Q000378:metabolism / Q000473:pathology*; D029082:Sendai virus / Q000235:genetics; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism
chemical_list: D014157:Transcription Factors