title: Estrogen Protects the Female Heart from Ischemia/Reperfusion Injury through Manganese Superoxide Dismutase Phosphorylation by Mitochondrial p38β at Threonine 79 and Serine 106.
abstract: A collective body of evidence indicates that estrogen protects the heart from myocardial ischemia/reperfusion (I/R) injury, but the underlying mechanism remains incompletely understood. We have previously delineated a novel mechanism of how 17β-estradiol (E2) protects cultured neonatal rat cardiomyocytes from hypoxia/reoxygenation (H/R) by identifying a functionally active mitochondrial pool of p38β and E2-driven upregulation of manganese superoxide dismutase (MnSOD) activity via p38β, leading to the suppression of reactive oxygen species (ROS) and apoptosis. Here we investigate these cytoprotective actions of E2 in vivo. Left coronary artery ligation and reperfusion was used to produce I/R injury in ovariectomized (OVX) female mice and in estrogen receptor (ER) null female mice. E2 treatment in OVX mice reduced the left ventricular infarct size accompanied by increased activity of mitochondrial p38β and MnSOD. I/R-induced infarct size in ERα knockout (ERKO), ERβ knockout (BERKO) and ERα and β double knockout (DERKO) female mice was larger than that in wild type (WT) mice, with little difference among ERKO, BERKO, and DERKO. Loss of both ERα and ERβ led to reduced activity of mitochondrial p38β and MnSOD at baseline and after I/R. The physical interaction between mitochondrial p38β and MnSOD in the heart was detected by co-immunoprecipitation (co-IP). Threonine 79 (T79) and serine 106 (S106) of MnSOD were identified to be phosphorylated by p38β in kinase assays. Overexpression of WT MnSOD in cardiomyocytes reduced ROS generation during H/R, while point mutation of T79 and S106 of MnSOD to alanine abolished its antioxidative function. We conclude that the protective effects of E2 and ER against cardiac I/R injury involve the regulation of MnSOD via posttranslational modification of the dismutase by p38β.
authors.affiliation: Division of Cardiology, Department of Medicine, School of Medicine, University of California Irvine, Irvine, California, United States of America.
authors.forename: Tao
authors.initials: T
authors.lastname: Luo
authors.affiliation: Division of Cardiology, Department of Medicine, School of Medicine, University of California Irvine, Irvine, California, United States of America.
authors.forename: Han
authors.initials: H
authors.lastname: Liu
authors.affiliation: Division of Cardiology, Department of Medicine, School of Medicine, University of California Irvine, Irvine, California, United States of America.
authors.forename: Jin Kyung
authors.initials: JK
authors.lastname: Kim
mesh_terms: D000818:Animals; D004958:Estradiol / Q000494:pharmacology*; D005260:Female; D008929:Mitochondria, Heart / Q000187:drug effects / Q000201:enzymology; D015428:Myocardial Reperfusion Injury / Q000201:enzymology / Q000517:prevention & control*; D010052:Ovariectomy; D010766:Phosphorylation; D051381:Rats; D017207:Rats, Sprague-Dawley; D012694:Serine / Q000378:metabolism*; D013482:Superoxide Dismutase / Q000378:metabolism*; D013912:Threonine / Q000378:metabolism*; D048051:p38 Mitogen-Activated Protein Kinases / Q000737:chemistry / Q000378:metabolism*
chemical_list: D013912:Threonine; D012694:Serine; D004958:Estradiol; D013482:Superoxide Dismutase; D048051:p38 Mitogen-Activated Protein Kinases