title: Natural Non-Mulberry Silk Nanoparticles for Potential-Controlled Drug Release.
abstract: Natural silk protein nanoparticles are a promising biomaterial for drug delivery due to their pleiotropic properties, including biocompatibility, high bioavailability, and biodegradability. Chinese oak tasarAntheraea pernyisilk fibroin (ApF) nanoparticles are easily obtained using cations as reagents under mild conditions. The mild conditions are potentially advantageous for the encapsulation of sensitive drugs and therapeutic molecules. In the present study, silk fibroin protein nanoparticles are loaded with differently-charged small-molecule drugs, such as doxorubicin hydrochloride, ibuprofen, and ibuprofen-Na, by simple absorption based on electrostatic interactions. The structure, morphology and biocompatibility of the silk nanoparticles in vitro are investigated. In vitro release of the drugs from the nanoparticles depends on charge-charge interactions between the drugs and the nanoparticles. The release behavior of the compounds from the nanoparticles demonstrates that positively-charged molecules are released in a more prolonged or sustained manner. Cell viability studies with L929 demonstrated that theApF nanoparticles significantly promoted cell growth. The results suggest that Chinese oak tasarAntheraea pernyisilk fibroin nanoparticles can be used as an alternative matrix for drug carrying and controlled release in diverse biomedical applications.
authors.affiliation: National Engineering Laboratory for Modern Silk, College of Textile and Clothing Engineering, Soochow University, Suzhou 215123, China. juanwang1006@126.com.|College of Chemistry and Chemical Engineering and Biological Engineering, Donghua University, Shanghai 201620, China. juanwang1006@126.com.
authors.forename: Juan
authors.initials: J
authors.lastname: Wang
authors.affiliation: National Engineering Laboratory for Modern Silk, College of Textile and Clothing Engineering, Soochow University, Suzhou 215123, China. 20154215016@stu.suda.edu.cn.
authors.forename: Zhuping
authors.initials: Z
authors.lastname: Yin
authors.affiliation: National Engineering Laboratory for Modern Silk, College of Textile and Clothing Engineering, Soochow University, Suzhou 215123, China. 20145215028@stu.suda.edu.cn.
authors.forename: Xiang
authors.initials: X
authors.lastname: Xue
authors.affiliation: Department of Biotechnology, Indian Institute of Technology Kharagpur, West Bengal 721302, India. kundu@hijli.iitkgp.ernet.in.
authors.forename: Subhas C
authors.initials: SC
authors.lastname: Kundu
authors.affiliation: College of Chemistry and Chemical Engineering and Biological Engineering, Donghua University, Shanghai 201620, China. xmm@dhu.edu.cn.
authors.forename: Xiumei
authors.initials: X
authors.lastname: Mo
authors.affiliation: National Engineering Laboratory for Modern Silk, College of Textile and Clothing Engineering, Soochow University, Suzhou 215123, China. lushenzhou@suda.edu.cn.
authors.forename: Shenzhou
authors.initials: S
authors.lastname: Lu
mesh_terms: D000818:Animals; D012831:Bombyx / Q000737:chemistry; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects; D003692:Delayed-Action Preparations; D004317:Doxorubicin / Q000008:administration & dosage / Q000737:chemistry; D016503:Drug Delivery Systems; D065546:Drug Liberation; D005349:Fibroins / Q000008:administration & dosage / Q000737:chemistry*; D006801:Humans; D007052:Ibuprofen / Q000008:administration & dosage / Q000737:chemistry; D053758:Nanoparticles / Q000737:chemistry* / Q000627:therapeutic use; D010316:Particle Size; D047011:Silk / Q000737:chemistry
chemical_list: D003692:Delayed-Action Preparations; D047011:Silk; D004317:Doxorubicin; D005349:Fibroins; D007052:Ibuprofen
keywords: Antheraea pernyi silk fibroin; controlled drug release; nanoparticles