title: Assessing the Off-Target Effects of miRNA Inhibitors on Innate Immune Toll-Like Receptors.
abstract: MicroRNAs (miRNAs) are involved in most cellular processes and are deregulated in several diseases. Antisense miRNA oligonucleotides (AMOs) therefore present novel therapeutic opportunities. Currently, in vivo delivery of AMOs often relies on high doses of nucleic acids, with nonspecific uptake by most tissues. Critically, AMOs accumulate in phagocytic cells where they can interfere with immune functions, such as the activation of Toll-Like Receptors (TLRs). In this chapter, we describe a method to assess the possible off-target effects of AMOs on TLR7, 8, and 9 sensing.
authors.affiliation: Centre for Cancer Research, Hudson Institute of Medical Research, 27-31 Wright St., Clayton, VIC, 3168, Australia.|Department of Molecular and Translational Science, Monash University, Clayton, VIC, 3168, Australia.
authors.forename: Geneviève
authors.initials: G
authors.lastname: Pépin
authors.affiliation: Centre for Cancer Research, Hudson Institute of Medical Research, 27-31 Wright St., Clayton, VIC, 3168, Australia.|Department of Molecular and Translational Science, Monash University, Clayton, VIC, 3168, Australia.
authors.forename: Jonathan
authors.initials: J
authors.lastname: Ferrand
authors.affiliation: Centre for Cancer Research, Hudson Institute of Medical Research, 27-31 Wright St., Clayton, VIC, 3168, Australia. michael.gantier@hudson.org.au.|Department of Molecular and Translational Science, Monash University, Clayton, VIC, 3168, Australia. michael.gantier@hudson.org.au.
authors.forename: Michael P
authors.initials: MP
authors.lastname: Gantier
mesh_terms: D006801:Humans; D007113:Immunity, Innate / Q000235:genetics*; D035683:MicroRNAs / Q000037:antagonists & inhibitors / Q000235:genetics*; D008967:Molecular Biology / Q000379:methods*; D016376:Oligonucleotides, Antisense / Q000235:genetics* / Q000627:therapeutic use; D010586:Phagocytes / Q000276:immunology / Q000378:metabolism; D051199:Toll-Like Receptor 7 / Q000235:genetics; D051200:Toll-Like Receptor 8 / Q000235:genetics; D051217:Toll-Like Receptor 9 / Q000235:genetics
chemical_list: D035683:MicroRNAs; D016376:Oligonucleotides, Antisense; D051199:Toll-Like Receptor 7; D051200:Toll-Like Receptor 8; D051217:Toll-Like Receptor 9
keywords: AMO; TLR7; TLR8; TLR9; Toll-like receptors; microRNA