title: 3D bioprinting of functional human skin: production and in vivo analysis.
abstract: Significant progress has been made over the past 25 years in the development of in vitro-engineered substitutes that mimic human skin, either to be used as grafts for the replacement of lost skin, or for the establishment of in vitro human skin models. In this sense, laboratory-grown skin substitutes containing dermal and epidermal components offer a promising approach to skin engineering. In particular, a human plasma-based bilayered skin generated by our group, has been applied successfully to treat burns as well as traumatic and surgical wounds in a large number of patients in Spain. There are some aspects requiring improvements in the production process of this skin; for example, the relatively long time (three weeks) needed to produce the surface required to cover an extensive burn or a large wound, and the necessity to automatize and standardize a process currently performed manually. 3D bioprinting has emerged as a flexible tool in regenerative medicine and it provides a platform to address these challenges. In the present study, we have used this technique to print a human bilayered skin using bioinks containing human plasma as well as primary human fibroblasts and keratinocytes that were obtained from skin biopsies. We were able to generate 100 cm2, a standard P100 tissue culture plate, of printed skin in less than 35 min (including the 30 min required for fibrin gelation). We have analysed the structure and function of the printed skin using histological and immunohistochemical methods, both in 3D in vitro cultures and after long-term transplantation to immunodeficient mice. In both cases, the generated skin was very similar to human skin and, furthermore, it was indistinguishable from bilayered dermo-epidermal equivalents, handmade in our laboratories. These results demonstrate that 3D bioprinting is a suitable technology to generate bioengineered skin for therapeutical and industrial applications in an automatized manner.
authors.affiliation: Department of Bioengineering and Aerospace Engineering, Universidad Carlos III de Madrid (UC3M), Spain.
authors.forename: Nieves
authors.initials: N
authors.lastname: Cubo
authors.forename: Marta
authors.initials: M
authors.lastname: Garcia
authors.forename: Juan F
authors.initials: JF
authors.lastname: Del Cañizo
authors.forename: Diego
authors.initials: D
authors.lastname: Velasco
authors.forename: Jose L
authors.initials: JL
authors.lastname: Jorcano
mesh_terms: D000818:Animals; D062028:Bioprinting / Q000379:methods*; D002454:Cell Differentiation; D002470:Cell Survival; D002478:Cells, Cultured; D005337:Fibrin / Q000737:chemistry; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism; D006801:Humans; D015603:Keratinocytes / Q000166:cytology / Q000378:metabolism; D051379:Mice; D008819:Mice, Nude; D019736:Prostheses and Implants; D012038:Regeneration; D012867:Skin / Q000473:pathology*; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D005337:Fibrin