title: Genome-Wide Analysis of RAS/ERK Signaling Targets.
abstract: Identifying gene expression changes mediated by signaling pathways is necessary to determine mechanisms that cause phenotypic change. Recent advances in next-generation sequencing and informatic pipelines have streamlined the ability for laboratories to create and analyze transcriptomic data. Here, we describe the preparation of samples and transcriptomic analysis in order to determine gene expression programs regulated by RAS/ERK signaling.
authors.affiliation: Department of Biology, Indiana University, Bloomington, IN, USA.
authors.forename: Joshua P
authors.initials: JP
authors.lastname: Plotnik
authors.affiliation: Medical Sciences, Indiana University School of Medicine, 1001 E 3rd St., Jordan Hall 104, Bloomington, IN, 47405, USA. pchollen@indiana.edu.
authors.forename: Peter C
authors.initials: PC
authors.lastname: Hollenhorst
mesh_terms: D019295:Computational Biology / Q000379:methods; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism*; D020869:Gene Expression Profiling; D055106:Genome-Wide Association Study / Q000379:methods; D059014:High-Throughput Nucleotide Sequencing; D006801:Humans; D015398:Signal Transduction; D059467:Transcriptome; D018631:ras Proteins / Q000378:metabolism*
chemical_list: D048049:Extracellular Signal-Regulated MAP Kinases; D018631:ras Proteins
keywords: Bioinformatics; Differential gene expression; Library preparation; Multiplex; RAS/ERK; RNA-seq; Transcription