title: Fluorescent probe based subcellular distribution of Cu(II) ions in living electrotrophs isolated from Cu(II)-reduced biocathodes of microbial fuel cells.
abstract: Based on the four indigenous electrotrophs (Stenotrophomonas maltophilia JY1, Citrobacter sp. JY3, Pseudomonas aeruginosa JY5 and Stenotrophomonas sp. JY6) isolated from well adapted Cu(II)-reduced biocathodes of microbial fuel cells (MFCs), a rhodamine based Cu(II) fluorescent probe was used to imaginably and quantitatively track subcellular Cu(II) ions in these electrotrophs. Cathodic electrons led to more Cu(II) ions (14.3-30.1%) in the intracellular sites at operation time of 2-3h with Cu(II) removal rates of 2.90-3.64mg/Lh whereas the absence of cathodic electrons prolonged the appearance of more Cu(II) ions (16.6-22.5%) to 5h with Cu(II) removal rates of 1.96-2.28mg/Lh. This study illustrates that cathodic electrons directed more Cu(II) ions for quicker entrance into the electrotrophic cytoplasm, and gives an alternative approach for developing imaging and functionally tracking Cu(II) ions in the electrotrophs of MFCs.
authors.affiliation: Key Laboratory of Industrial Ecology and Environmental Engineering, Ministry of Education (MOE), School of Environmental Science and Technology, Dalian University of Technology, Dalian 116024, China.
authors.forename: Ye
authors.initials: Y
authors.lastname: Tao
authors.affiliation: Key Laboratory of Industrial Ecology and Environmental Engineering, Ministry of Education (MOE), School of Environmental Science and Technology, Dalian University of Technology, Dalian 116024, China.
authors.forename: Hua
authors.initials: H
authors.lastname: Xue
authors.affiliation: Key Laboratory of Industrial Ecology and Environmental Engineering, Ministry of Education (MOE), School of Environmental Science and Technology, Dalian University of Technology, Dalian 116024, China. Electronic address: lipinghuang@dlut.edu.cn.
authors.forename: Liping
authors.initials: L
authors.lastname: Huang
authors.affiliation: College of Chemistry, Dalian University of Technology, Dalian 116024, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Zhou
authors.affiliation: State Key Laboratory of Fine Chemicals, Dalian University of Technology, Dalian 116024, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Yang
authors.affiliation: Key Laboratory of Industrial Ecology and Environmental Engineering, Ministry of Education (MOE), School of Environmental Science and Technology, Dalian University of Technology, Dalian 116024, China.
authors.forename: Xie
authors.initials: X
authors.lastname: Quan
authors.affiliation: Key Laboratory of Industrial Ecology and Environmental Engineering, Ministry of Education (MOE), School of Environmental Science and Technology, Dalian University of Technology, Dalian 116024, China.
authors.forename: Jinxiu
authors.initials: J
authors.lastname: Yuan
mesh_terms: D001674:Bioelectric Energy Sources; D003300:Copper / Q000032:analysis / Q000737:chemistry / Q000378:metabolism; D004566:Electrodes; D004583:Electrons; D005456:Fluorescent Dyes / Q000032:analysis / Q000737:chemistry / Q000378:metabolism; D006090:Gram-Negative Bacteria / Q000737:chemistry / Q000378:metabolism; D042541:Intracellular Space / Q000737:chemistry / Q000378:metabolism; D012235:Rhodamines / Q000032:analysis / Q000737:chemistry / Q000378:metabolism
chemical_list: D005456:Fluorescent Dyes; D012235:Rhodamines; D003300:Copper
keywords: Biocathode; Cu(II) recovery; Electrotroph; Microbial fuel cell; Rhodamine based Cu(II) fluorescent probe