title: Generation of human iPSCs from urine derived cells of a patient with a novel homozygous PAI-1 mutation.
abstract: We have generated a human induced pluripotent stem cell (iPSC) line under feeder-free culture conditions using the urine derived cells (UCs) collected from subject with a novel homozygous Plasminogen Activator Inhibitor-1 (PAI-1 null) mutation. The Sendai virus (SeV) vector encoding pluripotent Yamanaka transcription factors was used at a low multiplicity of infection to reprogram the PAI-1 UCs.
authors.affiliation: Department of Medicine and Cardiovascular Center, Medical College of Wisconsin, Milwaukee, WI, United States.
authors.forename: Muhammad Zeeshan
authors.initials: MZ
authors.lastname: Afzal
authors.affiliation: Department of Medicine and Cardiovascular Center, Medical College of Wisconsin, Milwaukee, WI, United States.
authors.forename: Melanie
authors.initials: M
authors.lastname: Gartz
authors.affiliation: Department of Medicine, Northwestern University-Feinberg School of Medicine, Chicago, IL, United States.
authors.forename: Ekaterina A
authors.initials: EA
authors.lastname: Klyachko
authors.affiliation: Department of Medicine, Northwestern University-Feinberg School of Medicine, Chicago, IL, United States.
authors.forename: Sadiya Sana
authors.initials: SS
authors.lastname: Khan
authors.affiliation: Department of Medicine, Northwestern University-Feinberg School of Medicine, Chicago, IL, United States.
authors.forename: Sanjiv J
authors.initials: SJ
authors.lastname: Shah
authors.affiliation: Indiana Hemophilia and Thrombosis Center, Indianapolis, IN, United States.
authors.forename: Sweta
authors.initials: S
authors.lastname: Gupta
authors.affiliation: Indiana Hemophilia and Thrombosis Center, Indianapolis, IN, United States.
authors.forename: Amy D
authors.initials: AD
authors.lastname: Shapiro
authors.affiliation: Department of Medicine, Northwestern University-Feinberg School of Medicine, Chicago, IL, United States.
authors.forename: Douglas E
authors.initials: DE
authors.lastname: Vaughan
authors.affiliation: Department of Medicine and Cardiovascular Center, Medical College of Wisconsin, Milwaukee, WI, United States. Electronic address: jstrande@mcw.edu.
authors.forename: Jennifer L
authors.initials: JL
authors.lastname: Strande
mesh_terms: D001483:Base Sequence; D002454:Cell Differentiation; D002460:Cell Line; D065150:Cellular Reprogramming; D004252:DNA Mutational Analysis; D058732:Embryoid Bodies / Q000166:cytology / Q000378:metabolism; D005260:Female; D005822:Genetic Vectors / Q000235:genetics / Q000378:metabolism; D006720:Homozygote; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000166:cytology* / Q000378:metabolism; D059785:Karyotype; D008856:Microscopy, Fluorescence; D016254:Mutagenesis, Insertional; D017395:Plasminogen Activator Inhibitor 1 / Q000235:genetics*; D029082:Sendai virus / Q000235:genetics; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D014556:Urine / Q000166:cytology
chemical_list: D017395:Plasminogen Activator Inhibitor 1; D014157:Transcription Factors