title: "Suppressor Factor" of Neutrophils: A Short Story of a Long-Term Misconception.
abstract: A large body of evidence obtained during the last decade has demonstrated that neutrophils suppress T cell proliferation in different models of inflammation and cell interaction. The commonly used method for assessing cell proliferation and proliferation inhibition is measuring [3H]thymidine incorporation into cells. Earlier, we observed inhibition of [3H]thymidine uptake in experiments on neutrophil-mediated regulation of T cell response in tuberculosis immunity. Here, we used different types of proliferating cells to analyze the nature of the soluble "neutrophil factor" by a variety of methods (dialysis, HPLC, mass spectrometry, and NMR) and unambiguously demonstrated that neutrophils do not synthesize a specific factor inhibiting cell proliferation, but secrete high concentrations of extracellular thymidine that competitively inhibit [3H]thymidine incorporation. Although the physiological significance of thymidine secretion by neutrophils remains unknown, this phenomenon should be carefully considered when designing test systems for studying cell-cell interactions.
authors.affiliation: Central Research Institute for Tuberculosis, Moscow, 107564, Russia. iralinge@gmail.com.
authors.forename: I A
authors.initials: IA
authors.lastname: Linge
authors.forename: E V
authors.initials: EV
authors.lastname: Kondratieva
authors.forename: T K
authors.initials: TK
authors.lastname: Kondratieva
authors.forename: V A
authors.initials: VA
authors.lastname: Makarov
authors.forename: V I
authors.initials: VI
authors.lastname: Polshakov
authors.forename: O Yu
authors.initials: OY
authors.lastname: Savelyev
authors.forename: A S
authors.initials: AS
authors.lastname: Apt
mesh_terms: D000818:Animals; D002450:Cell Communication / Q000276:immunology*; D051379:Mice; D008808:Mice, Inbred CBA; D009504:Neutrophils / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology*