title: The use of an inkjet injection technique in immunoassays by quantitative on-line electrophoretically mediated microanalysis.
abstract: The paper describes a new online quantitative electrophoretically mediated microanalysis (EMMA) for use in immunoassays based on the unique drop-by-drop introduction of a sample by means of an inkjet for capillary electrophoresis (CE). Plugs of a fluorescein-labeled antibody (Anti-humanIgG-DyL550) and human IgG were alternately injected into a capillary using the inkjet, followed by the merging of the plugs and the subsequent immune reaction. The antigen-antibody complex that was formed in the merged zone was then separated by CE. As a proof-of-concept, the method was used to determine human IgG. As a result, both the consumption of the reaction solution and the analysis time were significantly reduced. The method showed a wide linear range (10-2000ngmL-1, R2=0.9912) of calibration and the detection limit (5ngmL-1) was substantially lower than that by for conventional methods.
authors.affiliation: Department of Applied Chemistry, Graduate School of Urban Environmental Sciences, Tokyo Metropolitan University, Minamiohsawa, Hachioji, Tokyo 192-0397, Japan.
authors.forename: Weifei
authors.initials: W
authors.lastname: Zhang
authors.affiliation: Department of Applied Chemistry, Graduate School of Urban Environmental Sciences, Tokyo Metropolitan University, Minamiohsawa, Hachioji, Tokyo 192-0397, Japan.
authors.forename: Sifeng
authors.initials: S
authors.lastname: Mao
authors.affiliation: Department of Applied Chemistry, Graduate School of Urban Environmental Sciences, Tokyo Metropolitan University, Minamiohsawa, Hachioji, Tokyo 192-0397, Japan.
authors.forename: Jianmin
authors.initials: J
authors.lastname: Yang
authors.affiliation: Department of Applied Chemistry, Graduate School of Urban Environmental Sciences, Tokyo Metropolitan University, Minamiohsawa, Hachioji, Tokyo 192-0397, Japan.
authors.forename: Hulie
authors.initials: H
authors.lastname: Zeng
authors.affiliation: Department of Applied Chemistry, Graduate School of Urban Environmental Sciences, Tokyo Metropolitan University, Minamiohsawa, Hachioji, Tokyo 192-0397, Japan.
authors.forename: Hizuru
authors.initials: H
authors.lastname: Nakajima
authors.affiliation: Department of Applied Chemistry, Graduate School of Urban Environmental Sciences, Tokyo Metropolitan University, Minamiohsawa, Hachioji, Tokyo 192-0397, Japan.
authors.forename: Shungo
authors.initials: S
authors.lastname: Kato
authors.affiliation: Department of Applied Chemistry, Graduate School of Urban Environmental Sciences, Tokyo Metropolitan University, Minamiohsawa, Hachioji, Tokyo 192-0397, Japan. Electronic address: uchiyama-katsumi@tmu.ac.jp.
authors.forename: Katsumi
authors.initials: K
authors.lastname: Uchiyama
mesh_terms: D002138:Calibration; D019075:Electrophoresis, Capillary / Q000379:methods*; D046209:Electrophoresis, Microchip / Q000379:methods*; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D007074:Immunoglobulin G / Q000032:analysis; D057230:Limit of Detection; D009862:Online Systems; D013997:Time Factors
chemical_list: D007074:Immunoglobulin G
keywords: Capillary electrophoresis; Electrophoretically mediated microanalysis; Inkjet technology; On-line immunoassay