title: [Estrogen exerts anti-inflammatory effects by inhibiting NF-κB pathway through binding with estrogen receptor β on synovicytes of osteoarthritis].
abstract: Objective To investigate the mechanism of estrogen's anti-inflammatory effects on synovial cells during the pathogenic process of osteoarthritis. Methods We isolated synovicytes from synovium tissues and identified the cells with flow cytometry. Then we detected the expression level of estrogen receptor β (ERβ) in synovicytes with immunofluorescence staining. The synovicytes were divided into control group, group pretreated with 10 ng/mL IL-1β, group pretreated with 10 ng/mL IL-1β and 10-7mol/L estrogen, group pretreated with 10 ng/mL IL-1β, 10-7mol/L estrogen and specific antagonist of ERβ, 10-5mol/L tetrahydrocannabinol (THC). Thirty-six hours later, we observed the mRNA and protein levels of IκBα, phospho-IκBα (p-IκBα) and IL-6. Results Immunofluorescence staining showed the high expression level of ERβ in synovicytes. In IL-1β treated cells, IL-6 mRNA and protein level, IκBα mRNA and p-IκBα protein levels were elevated compared with the control group, while IκBα protein level declined. In the cells pretreated with IL-1β and estrogen, the mRNA and protein levels of IL-6, IκBα and p-IκBα were inhibited compared with IL-1β treated cells. THC blocked the effects of estrogen on the IL-1β and estrogen treated cells, and the mRNA and protein levels of IL-6, IκBα and p-IκBα had no significant difference compared with IL-1β treated cells. Conclusion The estrogen can restrain the activation of NF-κB pathway in synovicytes via ERβ, thus playing a vital role in anti-inflammation.
authors.affiliation: Department of Orthopaedic Surgery, Tangdu Hospital, Fourth Military Medical University, Xi'an 710038, China.
authors.forename: Enwei
authors.initials: E
authors.lastname: Zhang
authors.affiliation: Department of Orthopaedic Surgery, Tangdu Hospital, Fourth Military Medical University, Xi'an 710038, China.
authors.forename: Hongtao
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Department of Cardiovascular Medicine, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China.
authors.forename: Fengzhou
authors.initials: F
authors.lastname: Liu
authors.affiliation: Department of Orthopaedic Surgery, Tangdu Hospital, Fourth Military Medical University, Xi'an 710038, China.
authors.forename: Chuan
authors.initials: C
authors.lastname: Dong
authors.affiliation: 53rd Unit in 77320 Army of PLA, Puer 665000, China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Yao
authors.affiliation: Department of Orthopaedic Surgery, Tangdu Hospital, Fourth Military Medical University, Xi'an 710038, China.
authors.forename: Zhe
authors.initials: Z
authors.lastname: Yun
authors.affiliation: Department of Teaching Affairs, 175th Hospital of PLA, Dongnan Affiliated Hospital of Xiamen University, Zhangzhou 363000, China.
authors.forename: Weiming
authors.initials: W
authors.lastname: Jian
authors.affiliation: Department of Orthopaedic Surgery, Tangdu Hospital, Fourth Military Medical University, Xi'an 710038, China. *Corresponding author, E-mail: gukemba@fmmu.edu.cn.
authors.forename: Bao'an
authors.initials: B
authors.lastname: Ma
mesh_terms: D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D002478:Cells, Cultured; D013759:Dronabinol / Q000494:pharmacology; D047629:Estrogen Receptor beta / Q000378:metabolism*; D004967:Estrogens / Q000494:pharmacology*; D006801:Humans; D020672:I-kappa B Proteins / Q000378:metabolism; D053583:Interleukin-1beta / Q000494:pharmacology; D015850:Interleukin-6 / Q000378:metabolism; D000072000:NF-KappaB Inhibitor alpha / Q000378:metabolism; D016328:NF-kappa B / Q000494:pharmacology*; D010003:Osteoarthritis / Q000378:metabolism*; D015398:Signal Transduction / Q000187:drug effects*; D000070918:Synoviocytes / Q000187:drug effects* / Q000378:metabolism*
chemical_list: D000893:Anti-Inflammatory Agents; D047629:Estrogen Receptor beta; D004967:Estrogens; D020672:I-kappa B Proteins; D053583:Interleukin-1beta; D015850:Interleukin-6; D016328:NF-kappa B; D000072000:NF-KappaB Inhibitor alpha; D013759:Dronabinol