title: Plasma and saliva miR-21 expression in colorectal cancer patients.
abstract: MicroRNA-21 (miR-21) expression was quantified by real-time qRT-PCR in peripheral blood and saliva samples obtained from patients diagnosed with colorectal cancer (CRC) of varying degrees of malignancy and healthy volunteers. All patients had adenocarcinoma located in the distal colon at different stages. Significant differences were detected between the control group and the total experimental group of CRC patients (plasma, P = 0.0001; saliva, P = 5e-12). MiR-21 expression was also significantly different in certain subgroups of patients with CRC disease stages II-IV as compared to the control group. No correlation of miR-21 expression was found with regard to gender and age of patents. Also, there were no significant individual correlations and linear regression of miR-21 expression in the plasma and saliva. The estimated diagnostic sensitivity and specificity of miR-21 expression were respectively 65 and 85% in the plasma, and 97 and 91% in the saliva. Our data suggest that miR-21 in both the saliva and plasma could be a proper biomarker for CRC screening, although the saliva miR-21 expression test looks preferable due to its higher sensitivity, specificity, and technical simplicity.
authors.affiliation: Department of Clinical Laboratory Diagnostics with a course of Molecular Medicine, First Pavlov State Medical University of St. Petersburg, 6/8 Lev Tolstoy Street, St. Petersburg, 197022, Russia. alexei.sazanov@technolog.edu.ru.|Department of the Molecular Biotechnology, Saint-Petersburg State Technological Institute (Technical University), St. Petersburg, Russia. alexei.sazanov@technolog.edu.ru.
authors.forename: A A
authors.initials: AA
authors.lastname: Sazanov
authors.affiliation: Department of Surgery and Emergency Medicine, First Pavlov State Medical University of St. Petersburg, St. Petersburg, Russia.
authors.forename: E V
authors.initials: EV
authors.lastname: Kiselyova
authors.affiliation: Department of Surgery and Emergency Medicine, First Pavlov State Medical University of St. Petersburg, St. Petersburg, Russia.
authors.forename: A A
authors.initials: AA
authors.lastname: Zakharenko
authors.affiliation: School of Biosciences, University of Kent, Canterbury, UK.
authors.forename: M N
authors.initials: MN
authors.lastname: Romanov
authors.affiliation: Department of Clinical Laboratory Diagnostics with a course of Molecular Medicine, First Pavlov State Medical University of St. Petersburg, 6/8 Lev Tolstoy Street, St. Petersburg, 197022, Russia.
authors.forename: M I
authors.initials: MI
authors.lastname: Zaraysky
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000032:analysis / Q000097:blood / Q000235:genetics; D016022:Case-Control Studies; D015179:Colorectal Neoplasms / Q000235:genetics*; D005260:Female; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000032:analysis / Q000097:blood / Q000235:genetics*; D008875:Middle Aged; D009367:Neoplasm Staging; D012463:Saliva / Q000737:chemistry*; D012680:Sensitivity and Specificity
chemical_list: D014408:Biomarkers, Tumor; C524940:MIRN21 microRNA, human; D035683:MicroRNAs
keywords: Blood plasma; Colorectal cancer; Epigenetics; Gene expression profiling; Molecular diagnostics; Real-time qRT-PCR; Saliva; miR-21; microRNA (miR)