title: [Letter to the Editor] Many commercial hot-start polymerases demonstrate activity prior to thermal activation.
abstract: Address correspondence to Martin A. Kennedy, Department of Pathology & Carney Centre for Pharmacogenomics, University of Otago, Christchurch, PO Box 4345, Christchurch, New Zealand. E-mail: martin.kennedy@otago.ac.nz.
authors.affiliation: Gene Structure and Function Laboratory, Department of Pathology, University of Otago, Christchurch, New Zealand.
authors.forename: Aaron J
authors.initials: AJ
authors.lastname: Stevens
authors.affiliation: Gene Structure and Function Laboratory, Department of Pathology, University of Otago, Christchurch, New Zealand.
authors.forename: Sarah
authors.initials: S
authors.lastname: Appleby
authors.affiliation: Gene Structure and Function Laboratory, Department of Pathology, University of Otago, Christchurch, New Zealand.
authors.forename: Martin A
authors.initials: MA
authors.lastname: Kennedy
mesh_terms: D004247:DNA / Q000032:analysis / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D004259:DNA-Directed DNA Polymerase / Q000737:chemistry* / Q000378:metabolism* / Q000592:standards; D006358:Hot Temperature; D016133:Polymerase Chain Reaction / Q000379:methods* / Q000592:standards
chemical_list: D004247:DNA; D004259:DNA-Directed DNA Polymerase
keywords: DNA amplification; PCR; hot-start polymerase; polymerase activity; primer-dimer; quality control