title: A novel monoclonal antibody suitable for the detection of leukotriene B4.
abstract: Leukotriene B4 as an inflammatory mediator is an important biomarker for different respiratory diseases like asthma, chronic obstructive pulmonary disease or cystic lung fibrosis. Therefore the detection of LTB4 is helpful in the diagnosis of these pulmonary diseases. However, until now its determination in exhaled breath condensates suffers from problems of accuracy. Reasons for that could be improper sample collection and preparation methods of condensates and the lack of consistently assay specificity and reproducibility of the used immunoassay detection system. In this study we describe the development and the characterization of a specific monoclonal antibody (S27BC6) against LTB4, its use as molecular recognition element for the development of an enzyme-linked immunoassay to detect LTB4 and discuss possible future diagnostic applications.
authors.affiliation: Chair of Immunotechnology, Department of Biotechnology, Institute of Biochemistry and Biology, University of Potsdam, Karl-Liebknecht-Str. 24-25, D-14476, Potsdam, Germany.
authors.forename: Steffi
authors.initials: S
authors.lastname: Lütkecosmann
authors.affiliation: FILT GmbH, Robert-Rössle-Str. 10, 13125 Berlin, Germany.
authors.forename: Axel
authors.initials: A
authors.lastname: Warsinke
authors.affiliation: FILT GmbH, Robert-Rössle-Str. 10, 13125 Berlin, Germany.
authors.forename: Winfried
authors.initials: W
authors.lastname: Tschöpe
authors.affiliation: FILT GmbH, Robert-Rössle-Str. 10, 13125 Berlin, Germany.
authors.forename: Rüdiger
authors.initials: R
authors.lastname: Eichler
authors.affiliation: Chair of Immunotechnology, Department of Biotechnology, Institute of Biochemistry and Biology, University of Potsdam, Karl-Liebknecht-Str. 24-25, D-14476, Potsdam, Germany. Electronic address: katja.hanack@uni-potsdam.de.
authors.forename: Katja
authors.initials: K
authors.lastname: Hanack
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000737:chemistry*; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D045853:Exhalation; D006241:Haptens / Q000737:chemistry; D006801:Humans; D006825:Hybridomas / Q000276:immunology / Q000378:metabolism; D007074:Immunoglobulin G / Q000737:chemistry; D020128:Inhibitory Concentration 50; D007975:Leukotriene B4 / Q000737:chemistry*; D051379:Mice; D008810:Mice, Inbred C57BL; D029424:Pulmonary Disease, Chronic Obstructive / Q000175:diagnosis / Q000628:therapy
chemical_list: D000911:Antibodies, Monoclonal; D006241:Haptens; D007074:Immunoglobulin G; D007975:Leukotriene B4
keywords: Chronic obstructive pulmonary disease (COPD); Hapten; Immunosensor; Leukotriene B4; Monoclonal antibody