title: Royal jelly supplemented soybean lecithin-based extenders improve post-thaw quality and incubation resilience of goat spermatozoa.
abstract: The aim of the present study was to evaluate different concentrations of royal jelly (RJ) supplemented extenders for post-thaw quality and incubation resilience of goat spermatozoa. Semen samples were collected from five goats. Pooled semen were diluted with soybean lecithin-based extender without RJ (control) or supplemented with different concentrations (0.25, 0.5 and 0.75%) of RJ (RJ0.25, RJ0.5, RJ0.75 respectively), at a final concentration of 150 × 106spermatozoon/mL. Semen samples were assessed for sperm motility, plasma membrane integrity using hypoosmotic swelling test (HOST) damaged acrosome using FITC-Pisum sativum agglutinin (PSA-FITC) and DNA integrity using terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL). The addition of RJ (0.5%, 0.75%) led to higher percentages of subjective motilities (55.33 ± 2.29%, 57.67 ± 2.58%) compared to control and RJ0.25 groups (49.00 ± 2,80%, 51.67 ± 3.09%) (P < 0.05) following the freeze-thawing process. RJ0.5 and RJ0.75 groups had higher plasma membrane functional integrities (66.40 ± 1.34%, 68.20 ± 2.05%) and lower defected acrosome rates (24.60 ± 3.36%, 23.80 ± 2.27%) compared to the other groups (P < 0.05). DNA damaged spermatozoa in all groups were not significant (P > 0.05). In the end of incubation, motility and HOST rates of RJ0.5 (14.00 ± 3.87%, 31.20 ± 3.70%) and RJ0.75 (15.00 ± 3.27%, 29.20 ± 2.59%) groups were higher than control (8.00 ± 2.54%, 18.20 ± 3.11%) and RJ0.25 (9.00 ± 2.07%, 20.60 ± 2.88%) groups (P < 0.05). Also defected acrosome and DNA fragmation rates of RJ0.5 (32.20 ± 1.30%, 5.4 ± 0.55%) and RJ0.75 (29.20 ± 1.30%, 5.80 ± 0.45%) groups were significantly lower than control (38.80 ± 0.84%, 7.40 ± 1.34%) and RJ0.25 (39.80 ± 2.05%, 7.00 ± 1.58) groups. This study shows that RJ supplemented extenders have beneficial effect on goat sperm parameters at 0 h and 6 h of incubation.
authors.affiliation: Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine, Uludag University, Gorukle, Bursa, 16059, Turkey. Electronic address: salcay@uludag.edu.tr.
authors.forename: Selim
authors.initials: S
authors.lastname: Alcay
authors.affiliation: Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine, Uludag University, Gorukle, Bursa, 16059, Turkey.
authors.forename: M Berk
authors.initials: MB
authors.lastname: Toker
authors.affiliation: Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine, Uludag University, Gorukle, Bursa, 16059, Turkey.
authors.forename: N Tekin
authors.initials: NT
authors.lastname: Onder
authors.affiliation: Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine, Uludag University, Gorukle, Bursa, 16059, Turkey.
authors.forename: Elif
authors.initials: E
authors.lastname: Gokce
mesh_terms: D000177:Acrosome / Q000502:physiology*; D000818:Animals; D002462:Cell Membrane / Q000502:physiology; D015925:Cryopreservation / Q000662:veterinary*; D003451:Cryoprotective Agents / Q000494:pharmacology*; D004249:DNA Damage / Q000187:drug effects; D005227:Fatty Acids / Q000494:pharmacology*; D006041:Goats; D020287:In Situ Nick-End Labeling; D007315:Insemination, Artificial / Q000662:veterinary; D054709:Lecithins / Q000494:pharmacology*; D008297:Male; D012661:Semen / Q000502:physiology; D012662:Semen Preservation / Q000662:veterinary*; D030262:Soybean Proteins / Q000494:pharmacology*; D013081:Sperm Motility / Q000502:physiology; D000099219:Royal Jelly
chemical_list: D003451:Cryoprotective Agents; D005227:Fatty Acids; D054709:Lecithins; D030262:Soybean Proteins; D000099219:Royal Jelly
keywords: Cryopreservation; Goat semen; Incubation resilience; Royal jelly