title: Y-chromosomal haplogroup distribution in the Tuzla Canton of Bosnia and Herzegovina: A concordance study using four different in silico assignment algorithms based on Y-STR data.
abstract: Y-chromosomal haplogroups are sets of ancestrally related paternal lineages, traditionally assigned by the use of Y-chromosomal single nucleotide polymorphism (Y-SNP) markers. An increasingly popular and a less labor-intensive alternative approach has been Y-chromosomal haplogroup assignment based on already available Y-STR data using a variety of different algorithms. In the present study, such in silico haplogroup assignments were made based on 23-loci Y-STR data for 100 unrelated male individuals from the Tuzla Canton, Bosnia and Herzegovina (B&H) using the following four different algorithms: Whit Athey's Haplogroup Predictor, Jim Cullen's World Haplogroup & Haplogroup-I Subclade Predictor, Vadim Urasin's YPredictor and the NevGen Y-DNA Haplogroup Predictor. Prior in-house assessment of these four different algorithms using a previously published dataset (n=132) from B&H with both Y-STR (12-loci) and Y-SNP data suggested haplogroup misassignment rates between 0.76% and 3.02%. Subsequent analyses with the Tuzla Canton population sample revealed only a few differences in the individual haplogroup assignments when using different algorithms. Nevertheless, the resultant Y-chromosomal haplogroup distribution by each method was very similar, where the most prevalent haplogroups observed were I, R and E with their sublineages I2a, R1a and E1b1b, respectively, which is also in accordance with the previously published Y-SNP data for the B&H population. In conclusion, results presented herein not only constitute a concordance study on the four most popular haplogroup assignment algorithms, but they also give a deeper insight into the inter-population differentiation in B&H on the basis of Y haplogroups for the first time.
authors.affiliation: Department of Genetics and Bioengineering, International Burch University, Francuske revolucije bb, 71210 Ilidža, Sarajevo, Bosnia and Herzegovina. Electronic address: serkan.dogan@ibu.edu.ba.
authors.forename: S
authors.initials: S
authors.lastname: Dogan
authors.affiliation: Department of Biology, Faculty of Natural Sciences and Mathematics, University of Tuzla, Univerzitetska 4, 75000 Tuzla, Bosnia and Herzegovina.
authors.forename: N
authors.initials: N
authors.lastname: Babic
authors.affiliation: Turkish Cypriot DNA Laboratory, Committee on Missing Persons in Cyprus Turkish Cypriot Member Office, Tanzimat Street No.: 204, Nicosia 99010, North Cyprus, Turkey; Dr. Fazıl Küçük Faculty of Medicine, Eastern Mediterranean University, Famagusta 99628, North Cyprus, Turkey.
authors.forename: C
authors.initials: C
authors.lastname: Gurkan
authors.affiliation: Department of Management, International Burch University, Francuske revolucije bb, 71210 Ilidža, Sarajevo, Bosnia and Herzegovina.
authors.forename: A
authors.initials: A
authors.lastname: Goksu
authors.affiliation: Department of Genetics and Bioengineering, International Burch University, Francuske revolucije bb, 71210 Ilidža, Sarajevo, Bosnia and Herzegovina; Institute for Anthropological Research, Ljudevita Gaja 32, 10000 Zagreb, Croatia.
authors.forename: D
authors.initials: D
authors.lastname: Marjanovic
authors.affiliation: Department of Biology, Faculty of Natural Sciences and Mathematics, University of Tuzla, Univerzitetska 4, 75000 Tuzla, Bosnia and Herzegovina.
authors.forename: V
authors.initials: V
authors.lastname: Hadziavdic
mesh_terms: D000465:Algorithms; D017522:Bosnia and Herzegovina; D041322:Chromosomes, Human, Y / Q000235:genetics*; D003198:Computer Simulation; D005828:Genetics, Population; D006239:Haplotypes; D006801:Humans; D008297:Male; D018895:Microsatellite Repeats; D020641:Polymorphism, Single Nucleotide