title: Assessing Vacuolar Escape of Listeria Monocytogenes.
abstract: Listeria monocytogenes is a bacterial pathogen which invades and multiplies within non-professional phagocytes. Signaling cascades involved in cellular entry have been extensively analyzed, but the events leading to vacuolar escape remain less clear. In this chapter, we detail a microscopy FRET-based assay which allows quantitatively measuring L. monocytogenes infection and escape from its internalization vacuole, as well as a correlative light/electron microscopy method to investigate the morphological features of the vacuolar compartments containing L. monocytogenes.
authors.affiliation: Institut Pasteur, Unité des Interactions Bactéries-Cellules, 25, rue du Docteur Roux, Paris, 75015, France.|INSERM, U604, Paris, 75015, France.|INRA, USC2020, Paris, 75015, France.
authors.forename: Juan J
authors.initials: JJ
authors.lastname: Quereda
authors.affiliation: Institut Pasteur, Ultrapole-CITech, Paris, 75015, France.
authors.forename: Martin
authors.initials: M
authors.lastname: Sachse
authors.affiliation: Institut Pasteur, Unité des Interactions Bactéries-Cellules, 25, rue du Docteur Roux, Paris, 75015, France.|INSERM, U604, Paris, 75015, France.|INRA, USC2020, Paris, 75015, France.|UMR CNRS 6023, Laboratoire Microorganismes: Génome Environnement, Université d'Auvergne, Clermont-Ferrand, 63000, France.
authors.forename: Damien
authors.initials: D
authors.lastname: Balestrino
authors.affiliation: Institut Pasteur, Unité des Interactions Bactéries-Cellules, 25, rue du Docteur Roux, Paris, 75015, France.|INSERM, U604, Paris, 75015, France.|INRA, USC2020, Paris, 75015, France.
authors.forename: Théodore
authors.initials: T
authors.lastname: Grenier
authors.affiliation: Institut Pasteur, Unité Dynamique des Interactions Hôte-Pathogène, Paris, 75015, France.
authors.forename: Jennifer
authors.initials: J
authors.lastname: Fredlund
authors.affiliation: Institut Pasteur, Imagopole-CITech, Paris, 75015, France.
authors.forename: Anne
authors.initials: A
authors.lastname: Danckaert
authors.affiliation: Institut Pasteur, Imagopole-CITech, Paris, 75015, France.
authors.forename: Nathalie
authors.initials: N
authors.lastname: Aulner
authors.affiliation: Institut Pasteur, Imagopole-CITech, Paris, 75015, France.
authors.forename: Spencer
authors.initials: S
authors.lastname: Shorte
authors.affiliation: Institut Pasteur, Unité Dynamique des Interactions Hôte-Pathogène, Paris, 75015, France.
authors.forename: Jost
authors.initials: J
authors.lastname: Enninga
authors.affiliation: Institut Pasteur, Unité des Interactions Bactéries-Cellules, 25, rue du Docteur Roux, Paris, 75015, France.|INSERM, U604, Paris, 75015, France.|INRA, USC2020, Paris, 75015, France.
authors.forename: Pascale
authors.initials: P
authors.lastname: Cossart
authors.affiliation: Institut Pasteur, Unité des Interactions Bactéries-Cellules, 25, rue du Docteur Roux, Paris, 75015, France. javier.pizarro-cerda@pasteur.fr.|INSERM, U604, Paris, 75015, France. javier.pizarro-cerda@pasteur.fr.|INRA, USC2020, Paris, 75015, France. javier.pizarro-cerda@pasteur.fr.
authors.forename: Javier
authors.initials: J
authors.lastname: Pizarro-Cerdá
mesh_terms: D001692:Biological Transport; D031541:Fluorescence Resonance Energy Transfer / Q000379:methods*; D008089:Listeria monocytogenes / Q000378:metabolism* / Q000648:ultrastructure*; D008854:Microscopy, Electron / Q000379:methods*; D008856:Microscopy, Fluorescence / Q000379:methods*; D014617:Vacuoles / Q000378:metabolism* / Q000648:ultrastructure
keywords: CCF4; Correlative light/electron microcopy (CLEM); Förster resonance energy transfer (FRET) microscopy; Listeria monocytogenes; Listeriolysin O; Phagocytosis; Phospholipases; Vacuole