title: A novel method for the rapid detection of microbes in blood using pleurocidin antimicrobial peptide functionalized piezoelectric sensor.
abstract: The rapid detection of microbes is critical in clinical diagnosis and food safety. Culture-dependent assays are the most widely used microbial detection methods, but these assays are time-consuming. In this study, a rapid microbial detection method was proposed using a pleurocidin/single-walled carbon nanotubes/interdigital electrode-multichannel series piezoelectric quartz crystal (pleurocidin/SWCNT/IDE-MSPQC) sensor. The selected pleurocidin antimicrobial peptide served as a recognition probe that exhibits broad-spectrum antimicrobial activity and the SWCNT acted as the electronic transducer and cross-linker for the immobilization of pleurocidin on the IDE. The response mechanism of the sensor was based on the specific interaction between pleurocidin and the microbe causing pleurocidin to detach from the SWCNT modified IDE, resulting in a sensitive frequency shift response of the MSPQC. Microbes that may be clinically present in the bloodstream during an infection were successfully detected by the proposed method within 15min. The developed strategy provides a new universal platform for the rapid detection of microbes.
authors.affiliation: State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, China; Department of Chemistry, Taiyuan Normal University, Taiyuan 030000, China. Electronic address: xiaohongshixhs@163.com.
authors.forename: Xiaohong
authors.initials: X
authors.lastname: Shi
authors.affiliation: State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, China.
authors.forename: Xiaoqing
authors.initials: X
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, China.
authors.forename: Qiongqiong
authors.initials: Q
authors.lastname: Yao
authors.affiliation: State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, China. Electronic address: fengjiaohe@hnu.edu.cn.
authors.forename: Fengjiao
authors.initials: F
authors.lastname: He
mesh_terms: D016470:Bacteremia / Q000175:diagnosis*; D015374:Biosensing Techniques; D002176:Candida albicans / Q000302:isolation & purification; D004311:Double-Blind Method; D016972:Enterobacter cloacae / Q000302:isolation & purification; D013293:Enterococcus faecalis / Q000302:isolation & purification; D004926:Escherichia coli / Q000302:isolation & purification; D029941:Fish Proteins / Q000494:pharmacology*; D016469:Fungemia / Q000175:diagnosis*; D006801:Humans; D007711:Klebsiella pneumoniae / Q000302:isolation & purification; D008826:Microbial Sensitivity Tests; D008962:Models, Theoretical; D037742:Nanotubes, Carbon / Q000737:chemistry; D011550:Pseudomonas aeruginosa / Q000302:isolation & purification; D013211:Staphylococcus aureus / Q000302:isolation & purification; D013296:Streptococcus pneumoniae / Q000302:isolation & purification
chemical_list: D029941:Fish Proteins; D037742:Nanotubes, Carbon; C105851:pleurocidin
keywords: Microbial detection; Piezoelectric sensor; Pleurocidin antimicrobial peptide