title: Comparison of the glycosphingolipids of human-induced pluripotent stem cells and human embryonic stem cells.
abstract: High expectations are held for human-induced pluripotent stem cells (hiPSC) since they are established from autologous tissues thus overcoming the risk of allogeneic immune rejection when used in regenerative medicine. However, little is known regarding the cell-surface carbohydrate antigen profile of hiPSC compared with human embryonic stem cells (hESC). Here, glycosphingolipids were isolated from an adipocyte-derived hiPSC line, and hiPSC and hESC glycosphingolipids were compared by concurrent characterization by binding assays with carbohydrate-recognizing ligands and mass spectrometry. A high similarity between the nonacid glycosphingolipids of hiPSC and hESC was found. The nonacid glycosphingolipids P1 pentaosylceramide, x2 pentaosylceramide and H type 1 heptaosylceramide, not previously described in human pluripotent stem cells (hPSC), were characterized in both hiPSC and hESC. The composition of acid glycosphingolipids differed, with increased levels of GM3 ganglioside, and reduced levels of GD1a/GD1b in hiPSC when compared with hESC. In addition, the hESC glycosphingolipids sulf-globopentaosylceramide and sialyl-globotetraosylceramide were lacking in hiPSC. Neural stem cells differentiating from hiPSC had a reduced expression of sialyl-lactotetra, whereas expression of the GD1a ganglioside was significantly increased. Thus, while sialyl-lactotetra is a marker of undifferentiated hPSC, GD1a is a novel marker of neural differentiation.
authors.affiliation: Institute of Clinical Sciences, Department of Surgery, S-41 345 Göteborg, Sweden.
authors.forename: Karin
authors.initials: K
authors.lastname: Säljö
authors.affiliation: Institute of Biomedicine, Department of Medical Biochemistry and Cell Biology, The Sahlgrenska Academy, University of Gothenburg, Göteborg, Sweden.
authors.forename: Angela
authors.initials: A
authors.lastname: Barone
authors.affiliation: Institute of Biomedicine, Department of Medical Biochemistry and Cell Biology, The Sahlgrenska Academy, University of Gothenburg, Göteborg, Sweden.
authors.forename: Dzeneta
authors.initials: D
authors.lastname: Vizlin-Hodzic
authors.affiliation: Institute of Biomedicine, Department of Medical Biochemistry and Cell Biology, The Sahlgrenska Academy, University of Gothenburg, Göteborg, Sweden.
authors.forename: Bengt R
authors.initials: BR
authors.lastname: Johansson
authors.affiliation: Institute of Clinical Sciences, Department of Surgery, S-41 345 Göteborg, Sweden.
authors.forename: Michael E
authors.initials: ME
authors.lastname: Breimer
authors.affiliation: Institute of Biomedicine, Department of Medical Biochemistry and Cell Biology, The Sahlgrenska Academy, University of Gothenburg, Göteborg, Sweden.
authors.forename: Keiko
authors.initials: K
authors.lastname: Funa
authors.affiliation: Institute of Biomedicine, Department of Medical Biochemistry and Cell Biology, The Sahlgrenska Academy, University of Gothenburg, Göteborg, Sweden.
authors.forename: Susann
authors.initials: S
authors.lastname: Teneberg
mesh_terms: D018929:Cell Culture Techniques; D002454:Cell Differentiation / Q000235:genetics*; D006028:Glycosphingolipids / Q000145:classification / Q000235:genetics* / Q000378:metabolism; D000066449:Human Embryonic Stem Cells / Q000378:metabolism*; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000378:metabolism*; D013058:Mass Spectrometry; D058953:Neural Stem Cells / Q000378:metabolism
chemical_list: D006028:Glycosphingolipids
keywords: ganglioside; glycosphingolipid structure; human embryonic stem cells; human-induced pluripotent stem cells; mass spectrometry