title: Role and mechanism of the AMPK pathway in waterborne Zn exposure influencing the hepatic energy metabolism of Synechogobius hasta.
abstract: Previous studies have investigated the physiological responses in the liver of Synechogobius hasta exposed to waterborne zinc (Zn). However, at present, very little is known about the underlying molecular mechanisms of these responses. In this study, RNA sequencing (RNA-seq) was performed to analyse the differences in the hepatic transcriptomes between control and Zn-exposed S. hasta. A total of 36,339 unigenes and 1,615 bp of unigene N50 were detected. These genes were further annotated to the Nonredundant protein (NR), Nonredundant nucleotide (Nt), Swiss-Prot, Kyoto Encyclopedia of Genes and Genomes (KEGG), Clusters of Orthologous Groups (COG) and Gene Ontology (GO) databases. After 60 days of Zn exposure, 708 and 237 genes were significantly up- and down-regulated, respectively. Many differentially expressed genes (DEGs) involved in energy metabolic pathways were identified, and their expression profiles suggested increased catabolic processes and reduced biosynthetic processes. These changes indicated that waterborne Zn exposure increased the energy production and requirement, which was related to the activation of the AMPK signalling pathway. Furthermore, using the primary hepatocytes of S. hasta, we identified the role of the AMPK signalling pathway in Zn-influenced energy metabolism.
authors.affiliation: Key Laboratory of Freshwater Animal Breeding, Ministry of Agriculture of P.R.C., Fishery College, Huazhong Agricultural University, Wuhan 430070, China.
authors.forename: Kun
authors.initials: K
authors.lastname: Wu
authors.affiliation: Key Laboratory of Freshwater Animal Breeding, Ministry of Agriculture of P.R.C., Fishery College, Huazhong Agricultural University, Wuhan 430070, China.
authors.forename: Chao
authors.initials: C
authors.lastname: Huang
authors.affiliation: Key Laboratory of Freshwater Animal Breeding, Ministry of Agriculture of P.R.C., Fishery College, Huazhong Agricultural University, Wuhan 430070, China.
authors.forename: Xi
authors.initials: X
authors.lastname: Shi
authors.affiliation: Key Laboratory of Freshwater Animal Breeding, Ministry of Agriculture of P.R.C., Fishery College, Huazhong Agricultural University, Wuhan 430070, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Chen
authors.affiliation: Key Laboratory of Freshwater Animal Breeding, Ministry of Agriculture of P.R.C., Fishery College, Huazhong Agricultural University, Wuhan 430070, China.
authors.forename: Yi-Huan
authors.initials: YH
authors.lastname: Xu
authors.affiliation: Key Laboratory of Freshwater Animal Breeding, Ministry of Agriculture of P.R.C., Fishery College, Huazhong Agricultural University, Wuhan 430070, China.
authors.forename: Ya-Xiong
authors.initials: YX
authors.lastname: Pan
authors.affiliation: Key Laboratory of Freshwater Animal Breeding, Ministry of Agriculture of P.R.C., Fishery College, Huazhong Agricultural University, Wuhan 430070, China.|Collaborative Innovation Center for Efficient and Health Production of Fisheries in Hunan Province, Changde 415000, China.
authors.forename: Zhi
authors.initials: Z
authors.lastname: Luo
authors.affiliation: Panjin Guanghe Crab Co., Ltd., Panjin 124201, China.
authors.forename: Xu
authors.initials: X
authors.lastname: Liu
mesh_terms: D055372:AMP-Activated Protein Kinases / Q000096:biosynthesis*; D000818:Animals; D004734:Energy Metabolism / Q000187:drug effects*; D029941:Fish Proteins / Q000096:biosynthesis*; D005399:Fishes; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects*; D022781:Hepatocytes / Q000378:metabolism*; D008099:Liver / Q000378:metabolism*; D015398:Signal Transduction / Q000187:drug effects*; D015032:Zinc / Q000494:pharmacology*
chemical_list: D029941:Fish Proteins; D055372:AMP-Activated Protein Kinases; D015032:Zinc