title: Cloning and characterization of adipogenin and its overexpression enhances fat accumulation of bovine myosatellite cells.
abstract: Adipogenin (ADIG) is an adipocyte-specific membrane protein highly expressed in adipose tissues and is increased during the adipocyte differentiation. However, the roles and mechanisms of ADIG on fat accumulation and adipocyte differentiation in ex vivo still largely unknown. In this study, we isolated bovine myosatellite cells based on adhesion characteristics to investigate whether ADIG overexpression could promote trans-differentiation and increase fat accumulation in myosatellite cells. Immunofluorescence labeling was then used for the phenotypic characteristics of myosatellite. Our results showed that, after induction of differentiation, adenovirus mediated ADIG overexpression could upregulate expression level of PPARγ, and Oil Red O staining showed larger lipid drops compared to control groups. In consistent, key components of Hh signaling pathway were down regulated when infected with ADIG adenovirus, even though treated with inhibitor of Hh signaling pathway together could not induce further decrease. In addition, bioinformatics analysis of ADIG was also performed for its structure and function.
authors.affiliation: Henan Collaborative Center of Molecular Diagnosis and Laboratory Medicine, Xinxiang Medical University, Xinxiang, Henan Province, 453003, China; Institute of Lung and Molecular Therapy, Xinxiang Medical University, Xinxiang, Henan Province, 453003, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Henan Collaborative Center of Molecular Diagnosis and Laboratory Medicine, Xinxiang Medical University, Xinxiang, Henan Province, 453003, China; School of Public Health, Xinxiang Medical University, Xinxiang, Henan Province, 453003, China. Electronic address: jiangbijie1001@163.com.
authors.forename: Bijie
authors.initials: B
authors.lastname: Jiang
authors.affiliation: College of Life Science and Technology, Dalian University, Dalian, Liaoning, 116622, China.
authors.forename: Changzhen
authors.initials: C
authors.lastname: Fu
authors.affiliation: College of Life Science, Xinyang Normal University, Xinyang, Henan, 464000, China.
authors.forename: Ruijie
authors.initials: R
authors.lastname: Hao
mesh_terms: D017667:Adipocytes / Q000166:cytology* / Q000378:metabolism*; D050156:Adipogenesis; D000818:Animals; D002417:Cattle; D002454:Cell Differentiation; D054338:Cell Transdifferentiation / Q000235:genetics / Q000502:physiology; D003001:Cloning, Molecular; D053823:Hedgehog Proteins / Q000235:genetics / Q000378:metabolism; D008565:Membrane Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D032496:Satellite Cells, Skeletal Muscle / Q000166:cytology* / Q000378:metabolism*; D015398:Signal Transduction; D015854:Up-Regulation
chemical_list: D053823:Hedgehog Proteins; D008565:Membrane Proteins; D011994:Recombinant Proteins
keywords: Bioinformatics analysis; Hh signaling pathway; Membrane protein; Trans-differentiation