title: MiR-433-3p suppresses cell growth and enhances chemosensitivity by targeting CREB in human glioma.
abstract: Previous studies reported that miR-433 exerts function widely in human tumorigenesis and development. Here, we further investigate the potential role of miR-433 in glioma. Quantitative real-time PCR demonstrated that miR-433-3p and miR-433-5p were low expressed in glioma tissues and cell lines. Functional studies suggested that the overexpression of miR-433-3p suppressed proliferation, induced apoptosis and inhibited invasion and migration of human glioma cells. But the growth and metastasis of glioma cells were not significantly influenced by overexpression of miR-433-5p. In a xenograft model, we also showed that miR-433-3p had an inhibitory effect on the growth of glioma. Bioinformatics coupled with luciferase and western blot assays revealed that CREB is a direct target of miR-433-3p, and the overexpression of CREB can rescue the phenotype changes induced by miR-433-3p overexpression. Besides, miR-433-3p could increase chemosensitivity of glioma to temozolomide by targeting CREB in vitro and in vivo. Taken together, these results suggest that miR-433-3p may function as a potential marker in diagnostic and therapeutic target for glioma.
authors.affiliation: Tianjin Key Laboratory of Cerebral Vascular and Neurodegenerative Diseases, Tianjin Neurosurgical Institute, Department of Neurosurgery, Tianjin Huanhu Hospital, Tianjin 300350, China.
authors.forename: Shupeng
authors.initials: S
authors.lastname: Sun
authors.affiliation: Tianjin Key Laboratory of Cerebral Vascular and Neurodegenerative Diseases, Tianjin Neurosurgical Institute, Department of Neurosurgery, Tianjin Huanhu Hospital, Tianjin 300350, China.|The Graduate School, Tianjin Medical University, Tianjin 300070, China.
authors.forename: Xiuyu
authors.initials: X
authors.lastname: Wang
authors.affiliation: Key Laboratory for Critical Care Medicine of the Ministry of Health, Tianjin First Center Hospital, Tianjin 300192, China.
authors.forename: Xinnv
authors.initials: X
authors.lastname: Xu
authors.affiliation: Department of Neurosurgery, Affiliated Hospital of Hebei University, Baoding 071000, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Di
authors.affiliation: The Graduate School, Tianjin Medical University, Tianjin 300070, China.
authors.forename: Jixiang
authors.initials: J
authors.lastname: Du
authors.affiliation: The Graduate School, Tianjin Medical University, Tianjin 300070, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Xu
authors.affiliation: Tianjin Key Laboratory of Cerebral Vascular and Neurodegenerative Diseases, Tianjin Neurosurgical Institute, Department of Neurosurgery, Tianjin Huanhu Hospital, Tianjin 300350, China.
authors.forename: Qiong
authors.initials: Q
authors.lastname: Wang
authors.affiliation: Tianjin Key Laboratory of Cerebral Vascular and Neurodegenerative Diseases, Tianjin Neurosurgical Institute, Department of Neurosurgery, Tianjin Huanhu Hospital, Tianjin 300350, China.
authors.forename: Jinhuan
authors.initials: J
authors.lastname: Wang
mesh_terms: D020413:3' Untranslated Regions; D000328:Adult; D001932:Brain Neoplasms / Q000188:drug therapy / Q000235:genetics*; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D017362:Cyclic AMP Response Element-Binding Protein / Q000235:genetics*; D003606:Dacarbazine / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000494:pharmacology; D015536:Down-Regulation; D019008:Drug Resistance, Neoplasm; D005260:Female; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D005910:Glioma / Q000188:drug therapy / Q000235:genetics*; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D008875:Middle Aged; D009361:Neoplasm Invasiveness; D009368:Neoplasm Transplantation; D000077204:Temozolomide; D055815:Young Adult
chemical_list: D020413:3' Untranslated Regions; C115163:CREB1 protein, human; D017362:Cyclic AMP Response Element-Binding Protein; C548883:MIRN433 microRNA, human; D035683:MicroRNAs; D003606:Dacarbazine; D000077204:Temozolomide
keywords: CREB; carcinogenesis; chemosensitivity; glioma; miR-433-3p