title: Biological properties of Hertia cheirifolia L. flower extracts and effect of the nopol on α-glucosidase.
abstract: In screening for antioxidant and α-glucosidase inhibitors from the extracts of Hertia cheirifolia L. flowers, the petroleum ether extract showed interesting antioxidant activity and inhibitory effect on the activity of α-glucosidase. The fractionation of this extract resulted in the isolation of a compound which is characterized by NMR and ESI-MS as a nopol. The nopol exhibited potent α-glucosidase inhibitory potential with IC50value of 220μM. The kinetic evaluation indicated that it acts as a non-competitive inhibitor. A molecular docking study proved that the nopol presented a strong affinity with amino acid residues of α-glucosidase.
authors.affiliation: Laboratory of Biochemistry, Research Unit: UR 12ES08 "Cell Signaling and Pathologies", Faculty of Medicine, University of Monastir, Tunisia. Electronic address: kaouther.bio@gmail.com.
authors.forename: Kaouther
authors.initials: K
authors.lastname: Majouli
authors.affiliation: Laboratory of Genome Diagnostics and Valorisation, ISBM, University of Monastir, Tunisia.
authors.forename: Mohamed Ali
authors.initials: MA
authors.lastname: Mahjoub
authors.affiliation: Department of Chemistry, Hazara University, Mansehra, 21120, Khyber Pukhtunkhwa, Pakistan.
authors.forename: Fazal
authors.initials: F
authors.lastname: Rahim
authors.affiliation: Laboratory of Chemical, Galenic and Pharmacological Development of Drugs, Faculty of Pharmacy, University of Monastir, Tunisia.
authors.forename: Assia
authors.initials: A
authors.lastname: Hamdi
authors.affiliation: Department of Biochemistry, Computational Medicinal Chemistry Laboratory, UCSS, Abdul Wali Khan University Mardan, Pakistan.
authors.forename: Abdul
authors.initials: A
authors.lastname: Wadood
authors.affiliation: Laboratory of Transmissible Diseases and of Biologically Active Substances, MDT01, Faculty of Pharmacy, University of Monastir, Tunisia.
authors.forename: Malek
authors.initials: M
authors.lastname: Besbes Hlila
authors.affiliation: Laboratory of Biochemistry, Research Unit: UR 12ES08 "Cell Signaling and Pathologies", Faculty of Medicine, University of Monastir, Tunisia.
authors.forename: Abderraouf
authors.initials: A
authors.lastname: Kenani
mesh_terms: D019659:Asteraceae / Q000737:chemistry*; D001643:Bridged Bicyclo Compounds / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D035264:Flowers / Q000737:chemistry*; D065089:Glycoside Hydrolase Inhibitors / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D062105:Molecular Docking Simulation; D010936:Plant Extracts / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D011487:Protein Conformation; D000520:alpha-Glucosidases / Q000737:chemistry / Q000378:metabolism*
chemical_list: D001643:Bridged Bicyclo Compounds; D065089:Glycoside Hydrolase Inhibitors; D010936:Plant Extracts; D000520:alpha-Glucosidases; C000616956:nopol
keywords: Antioxidant activity; Molecular docking; α-Glucosidase inhibition