title: Transfer to forest nurseries significantly affects mycorrhizal community composition of Asteropeia mcphersonii wildings.
abstract: Mycorrhizal symbiosis is extremely important for tree growth, survival and resistance after transplantation particularly in Madagascar where deforestation is a major concern. The importance of mycorrhizal symbiosis is further increased when soil conditions at the planting site are limiting. To identify technical itineraries capable of improving ecological restoration in Madagascar, we needed to obtain native ectomycorrhizal (ECM) saplings with a wide diversity of ECM fungi. To this end, we transplanted ECM seedlings from the wild (wildlings) to a nursery. Using molecular characterisation of internal transcribed spacer (ITS) rDNA, we tested the effect of transplanting Asteropeia mcphersonii wildlings on ECM communities after 8 months of growth in the nursery. With or without the addition of soil from the site where the seedlings were sampled to the nursery substrate, we observed a dramatic change in the composition of fungal communities with a decrease in the ECM infection rate, a tremendous increase in the abundance of an operational taxonomic unit (OTU) taxonomically close to the order Trechisporales and the disappearance of all OTUs of Boletales. Transplanting to the nursery and/or to nursery conditions was shown to be incompatible with the survival and even less with the development in the nursery of most ECM fungi naturally associated with A. mcphersonii wildings.
authors.affiliation: AgroParisTech, Laboratoire des Symbioses Tropicales et Méditerranéennes, IRD/INRA/CIRAD/Montpellier SupAgro/Université Montpellier, TA10J, 34398, Montpellier Cedex 5, France.
authors.forename: Charline
authors.initials: C
authors.lastname: Henry
authors.affiliation: Ambatovy, Immeuble Tranofitaratra-7ème étage, rue Ravoninahitriniarivo-Ankorondrano, 101, Antananarivo, Madagascar.
authors.forename: Jeanne-Françoise
authors.initials: JF
authors.lastname: Raivoarisoa
authors.affiliation: Ambatovy, Immeuble Tranofitaratra-7ème étage, rue Ravoninahitriniarivo-Ankorondrano, 101, Antananarivo, Madagascar.
authors.forename: Angélo
authors.initials: A
authors.lastname: Razafimamonjy
authors.affiliation: Laboratoire de Microbiologie de l'environnement, Centre National de Recherches sur l'Environnement, Antananarivo, Madagascar.
authors.forename: Heriniaina
authors.initials: H
authors.lastname: Ramanankierana
authors.affiliation: Ambatovy, Immeuble Tranofitaratra-7ème étage, rue Ravoninahitriniarivo-Ankorondrano, 101, Antananarivo, Madagascar.
authors.forename: Paul
authors.initials: P
authors.lastname: Andrianaivomahefa
authors.affiliation: CIRAD, Laboratoire des Symbioses Tropicales et Méditerranéennes, IRD/INRA/CIRAD/Montpellier SupAgro/Université Montpellier, TA10C, 34398, Montpellier Cedex 5, France. marc.ducousso@cirad.fr.
authors.forename: Marc
authors.initials: M
authors.lastname: Ducousso
authors.affiliation: Institut de Systématique, Évolution, Biodiversité (ISYEB-UMR 7205-CNRS, MNHN, UPMC, EPHE), Muséum national d'Histoire naturelle, Sorbonne Universités, 57 rue Cuvier, CP50, 75005, Paris, France.|Department of Plant Taxonomy and Nature Conservation, University of Gdansk, Wita Stwosza 59, 80-308, Gdansk, Poland.
authors.forename: Marc-André
authors.initials: MA
authors.lastname: Selosse
mesh_terms: D001487:Basidiomycota; D000073559:Caryophyllales / Q000382:microbiology*; D016468:Forestry / Q000379:methods; D065928:Forests; D008270:Madagascar; D038821:Mycorrhizae / Q000145:classification*; D012988:Soil Microbiology; D013559:Symbiosis
keywords: Asteropeia mcphersonii; Ectomycorrhizal community; Forest nursery; Fungal ITS; Madagascar