title: A specific DNA-nanoprobe for tracking the activities of human apurinic/apyrimidinic endonuclease 1 in living cells.
abstract: Human apurinic/apyrimidinic endonuclease/redox effector factor 1 (APE1) is an essential DNA repair protein. Herein, we demonstrate that avidin-oriented abasic site-containing DNA strands (AP-DNA) on the surface of silica coated magnetic nanoparticles (SiMNP) can selectively respond to APE1 while resist the digestion by other nucleases. Mechanism studies have revealed that avidin may serve as an organizer protein and recruit APE1 to the DNA substrates on the nanoparticles via strong and specific interactions. Taking advantage of this newly disclosed property, we for the first time successfully displayed the intracellular activities of APE1 in living cells by fluorescence imaging. The avidin organized AP-DNA-SiMNP assembly holds great potential for enzyme-mediated release of drugs inside tumor cells which often contain higher levels of APE1 than normal cells.
authors.affiliation: Beijing National Laboratory for Molecular Sciences and MOE Key Laboratory of Bioorganic Chemistry and Molecular Engineering, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China.
authors.forename: Junqiu
authors.initials: J
authors.lastname: Zhai
authors.affiliation: Beijing National Laboratory for Molecular Sciences and MOE Key Laboratory of Bioorganic Chemistry and Molecular Engineering, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China.
authors.forename: Yibin
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Beijing National Laboratory for Molecular Sciences and MOE Key Laboratory of Bioorganic Chemistry and Molecular Engineering, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China.
authors.forename: Shan
authors.initials: S
authors.lastname: Huang
authors.affiliation: Beijing National Laboratory for Molecular Sciences and MOE Key Laboratory of Bioorganic Chemistry and Molecular Engineering, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China.
authors.forename: Simin
authors.initials: S
authors.lastname: Fang
authors.affiliation: Beijing National Laboratory for Molecular Sciences and MOE Key Laboratory of Bioorganic Chemistry and Molecular Engineering, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China.
authors.forename: Meiping
authors.initials: M
authors.lastname: Zhao
mesh_terms: D001360:Avidin; D053837:DNA Cleavage; D015342:DNA Probes; D043603:DNA-(Apurinic or Apyrimidinic Site) Lyase / Q000032:analysis* / Q000378:metabolism; D003850:Deoxyribonuclease I / Q000032:analysis / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D053768:Metal Nanoparticles; D061848:Optical Imaging
chemical_list: D015342:DNA Probes; D001360:Avidin; D003850:Deoxyribonuclease I; C085108:APEX1 protein, human; D043603:DNA-(Apurinic or Apyrimidinic Site) Lyase