title: Effects of Platelet-Rich Plasma on Kidney Regeneration in Gentamicin-Induced Nephrotoxicity.
abstract: Platelet-rich plasma (PRP) as a source of growth factors may induce tissue repairing and improve fibrosis. This study aimed to assess the effects of PRP on kidney regeneration and fibrosis in gentamicin (GM)-induced nephrotoxicity rat model by stereological study. Thirty-two male rats were selected. Nephrotoxicity was induced in animals by administration of GM (80 mg/kg/daily, intraperitoneally [IP], 8 day) and animals were treated by PRP (100 μL, intra-cortical injection using surgical microscopy, single dose). Blood samples were collected for determine blood urea nitrogen (BUN) and creatinine (Cr) before and after PRP therapy. At the end of experiment, right kidneys were sectioned by Isotropic Uniform Random (IUR) method and stained with H & E and Masson's Trichrome. The stereological methods were used for estimating the changes in different structures of kidney. PRP increased the number of epithelial cells in convoluted tubules, and decreased the volume of connective tissue, renal corpuscles and glomeruli in GM-treated animals (P < 0.05). Our findings indicate that PRP had beneficial effects on proliferation of epithelial cells in convoluted tubules and ameliorated GM-induced fibrosis.
authors.affiliation: Department of Anatomy, Shiraz University of Medical Sciences, Shiraz, Iran.|Histomorphometry and Stereology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
authors.forename: Abbas
authors.identifier: http://orcid.org/0000-0002-5107-5569
authors.initials: A
authors.lastname: Moghadam
authors.affiliation: Department of Anatomy, Shiraz University of Medical Sciences, Shiraz, Iran.
authors.forename: Tahereh Talaei
authors.identifier: http://orcid.org/0000-0002-8425-8871
authors.initials: TT
authors.lastname: Khozani
authors.affiliation: Department of Anatomy, Shiraz University of Medical Sciences, Shiraz, Iran.|Histomorphometry and Stereology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
authors.forename: Afsaneh
authors.identifier: http://orcid.org/0000-0002-7160-5088
authors.initials: A
authors.lastname: Mafi
authors.affiliation: Department of Anatomy, Shiraz University of Medical Sciences, Shiraz, Iran.|Histomorphometry and Stereology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
authors.forename: Mohammad Reza
authors.identifier: http://orcid.org/0000-0002-6635-2717
authors.initials: MR
authors.lastname: Namavar
authors.affiliation: Department of Anatomy, Shiraz University of Medical Sciences, Shiraz, Iran.|Histomorphometry and Stereology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran. dehghanf@sums.ac.ir.
authors.forename: Farzaneh
authors.identifier: http://orcid.org/0000-0003-2217-5672
authors.initials: F
authors.lastname: Dehghani
mesh_terms: D000818:Animals; D001806:Blood Urea Nitrogen; D003404:Creatinine / Q000097:blood; D005839:Gentamicins / Q000633:toxicity*; D036341:Intercellular Signaling Peptides and Proteins / Q000494:pharmacology; D007668:Kidney / Q000293:injuries / Q000473:pathology / Q000502:physiology*; D008297:Male; D010976:Platelet Count; D053657:Platelet-Rich Plasma / Q000166:cytology / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012038:Regeneration / Q000187:drug effects*
chemical_list: D005839:Gentamicins; D036341:Intercellular Signaling Peptides and Proteins; D003404:Creatinine
keywords: Fibrosis; Kidney; Regeneration