title: Oleuropein protects L-02 cells against H2O2-induced oxidative stress by increasing SOD1, GPx1 and CAT expression.
abstract: Oleuropein (OL), a natural phenolic compound, comprises the major constituent of Olea europea leaves and unprocessed olives, and OL is considered to be the principal components that confer the characteristic taste and stability of olive oil. Oxidative damage induced by H2O2treatment can irreversibly damage the L-02 cells, resulting in cell death and apoptosis. Whether the effects of oxidative stress could be attenuated in cultured human L-02 cells by incubation with OL is still unknown. In this research, the function of OL in protecting human L-02 cells against H2O2induced cell death and cell apoptosis was investigated, and the mechanism by which OL underlies the effect was also examed. L-02 cells were exposed to 100μM H2O2with or without OL pretreatment at different concentrations. Cell viabilities were monitored by WST-1 assay. ALT, AST and LDH production in culture medium were also determined. ROS levels were detected by L-012 chemiluminescence, and OL increased SOD1, CAT and GPx1 expression in a concentration-dependent manner. Further studies showed that OL also inhibited H2O2-induced P38 and JNK phosphorylation but enhanced ERK1/2 phosphorylation in a dose-dependent manner. These findings suggested that OL as a potent antioxidant agent and a natural compound found in several plants, may be exploited as a potentially useful method for hepatopathy prevention.
authors.affiliation: Department of Food Quality and Safety, College of Food Science and Engineering, Jilin University, 130062 Changchun, PR China.
authors.forename: Ce
authors.initials: C
authors.lastname: Shi
authors.affiliation: Department of Food Quality and Safety, College of Food Science and Engineering, Jilin University, 130062 Changchun, PR China.
authors.forename: Xiangrong
authors.initials: X
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, 130022, Changchun, PR China.
authors.forename: Zuojia
authors.initials: Z
authors.lastname: Liu
authors.affiliation: Jilin Entry-Exit Inspection and Quarantine Bureau, 130062, Changchun, PR China.
authors.forename: Rizeng
authors.initials: R
authors.lastname: Meng
authors.affiliation: Department of Food Quality and Safety, College of Food Science and Engineering, Jilin University, 130062 Changchun, PR China.
authors.forename: Xingchen
authors.initials: X
authors.lastname: Zhao
authors.affiliation: Department of Food Quality and Safety, College of Food Science and Engineering, Jilin University, 130062 Changchun, PR China.
authors.forename: Zonghui
authors.initials: Z
authors.lastname: Liu
authors.affiliation: Department of Food Quality and Safety, College of Food Science and Engineering, Jilin University, 130062 Changchun, PR China. Electronic address: abas1980@163.com.
authors.forename: Na
authors.initials: N
authors.lastname: Guo
mesh_terms: D002374:Catalase / Q000235:genetics / Q000378:metabolism*; D048049:Extracellular Signal-Regulated MAP Kinases / Q000235:genetics / Q000378:metabolism; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D005979:Glutathione Peroxidase / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D006861:Hydrogen Peroxide / Q000633:toxicity*; D057888:Iridoid Glucosides; D039823:Iridoids / Q000494:pharmacology*; D008099:Liver / Q000166:cytology; D048670:MAP Kinase Kinase 4 / Q000235:genetics / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects*; D010766:Phosphorylation; D000072105:Superoxide Dismutase-1 / Q000235:genetics / Q000378:metabolism*; D048051:p38 Mitogen-Activated Protein Kinases / Q000235:genetics / Q000378:metabolism; D000094765:Glutathione Peroxidase GPX1
chemical_list: D057888:Iridoid Glucosides; D039823:Iridoids; C000606290:SOD1 protein, human; C002769:oleuropein; D006861:Hydrogen Peroxide; D002374:Catalase; D005979:Glutathione Peroxidase; D000072105:Superoxide Dismutase-1; D048049:Extracellular Signal-Regulated MAP Kinases; D048051:p38 Mitogen-Activated Protein Kinases; D048670:MAP Kinase Kinase 4; D000094765:Glutathione Peroxidase GPX1; C000722067:GPX1 protein, human
keywords: Antioxidant enzymes; Apoptosis; H(2)O(2); Oleuropein; Oxidative damage