title: Insight in DNA Repair of UV-induced Pyrimidine Dimers by Chromatographic Methods.
abstract: UV-induced formation of pyrimidine dimers in DNA is a major deleterious event in both eukaryotic and prokaryotic cells. Accumulation of cyclobutane pyrimidine dimers and pyrimidine (6-4) pyrimidone photoproducts can lead to cell death or be at the origin of mutations. In skin, UV induction of DNA damage is a major initiating event in tumorigenesis. To counteract these deleterious effects, all cell types possess DNA repair machinery, such as nucleotide excision repair and, in some cell types, direct reversion. Different analytical approaches were used to assess the efficiency of repair and decipher the enzymatic mechanisms. We presently review the information provided by chromatographic methods, which are complementary to biochemical assays, such as immunological detection and electrophoresis-based techniques. Chromatographic assays are interesting in their ability to provide quantitative data on a wide range of damage and are also valuable tools for the identification of repair intermediates.
authors.affiliation: Univ. Grenoble Alpes, INAC, LCIB, LAN, Grenoble, France.|CEA, INAC, SyMMES, LAN, Grenoble, France.
authors.forename: Thierry
authors.initials: T
authors.lastname: Douki
authors.affiliation: Univ. Grenoble Alpes, INAC, LCIB, LAN, Grenoble, France.|CEA, INAC, SyMMES, LAN, Grenoble, France.
authors.forename: Anne
authors.initials: A
authors.lastname: von Koschembahr
authors.affiliation: Département de Médecine Nucléaire et Radiobiologie, Faculté de Médecine, Université de Sherbrooke, Sherbrooke, QC, Canada.
authors.forename: Jean
authors.initials: J
authors.lastname: Cadet
mesh_terms: D002851:Chromatography, High Pressure Liquid; D004249:DNA Damage; D004260:DNA Repair; D004255:Deoxyribodipyrimidine Photo-Lyase / Q000032:analysis; D004586:Electrophoresis; D011506:Proteins / Q000032:analysis; D011740:Pyrimidine Dimers / Q000032:analysis*; D012867:Skin / Q000378:metabolism / Q000528:radiation effects; D013050:Spectrometry, Fluorescence; D053719:Tandem Mass Spectrometry; D014466:Ultraviolet Rays
chemical_list: D011506:Proteins; D011740:Pyrimidine Dimers; C058406:pyrimidine-pyrimidone dimer; C088014:spore photoproduct lyase; D004255:Deoxyribodipyrimidine Photo-Lyase