title: High Aerobic Capacity Mitigates Changes in the Plasma Metabolomic Profile Associated with Aging.
abstract: Advancing age is associated with declines in maximal oxygen consumption. Declines in aerobic capacity not only contribute to the aging process but also are an independent risk factor for morbidity, cardiovascular disease, and all-cause mortality. Although statistically convincing, the relationships between aerobic capacity, aging, and disease risk remain largely unresolved. To this end, we employed sensitive, system-based metabolomics approach to determine whether enhanced aerobic capacity could mitigate some of the changes seen in the plasma metabolomic profile associated with aging. Metabolomic profiles of plasma samples obtained from young (13 month) and old (26 month) rats bred for low (LCR) or high (HCR) running capacity using proton nuclear magnetic resonance spectroscopy (1H NMR) were examined. Results demonstrated strong profile separation in old and low aerobic capacity rats, whereas young and high aerobic capacity rat models were less predictive. Significantly differential metabolites between the groups include taurine, acetone, valine, and trimethylamine-N-oxide among other metabolites, specifically citrate, succinate, isovalerate, and proline, were differentially increased in older HCR animals compared with their younger counterparts. When interactions between age and aerobic capacity were examined, results demonstrated that enhanced aerobic capacity could mitigate some but not all age-associated alterations in the metabolomic profile.
authors.affiliation: Department of Biological Sciences, Faculty of Science, University of Calgary , Calgary, Alberta T2N1N4, Canada.
authors.forename: Oluyemi S
authors.initials: OS
authors.lastname: Falegan
authors.affiliation: Department of Biological Sciences, Faculty of Science, University of Calgary , Calgary, Alberta T2N1N4, Canada.|Department of Biochemistry & Molecular Biology, Faculty of Medicine, University of Calgary , Calgary, Alberta T2N1N4, Canada.
authors.forename: Hans J
authors.initials: HJ
authors.lastname: Vogel
authors.affiliation: Department of Biochemistry & Molecular Biology, Faculty of Medicine, University of Calgary , Calgary, Alberta T2N1N4, Canada.|Faculty of Kinesiology, University of Calgary , Calgary, Alberta T2N1N4, Canada.
authors.forename: Dustin S
authors.initials: DS
authors.lastname: Hittel
authors.affiliation: Department of Anesthesiology, University of Michigan , Ann Arbor, Michigan 48109, United States.
authors.forename: Lauren G
authors.initials: LG
authors.lastname: Koch
authors.affiliation: Department of Anesthesiology, University of Michigan , Ann Arbor, Michigan 48109, United States.|K. G. Jebsen Center for Exercise in Medicine, Department of Circulation and Medical Imaging, Norwegian University of Science and Technology , Trondheim NO-7491, Norway.
authors.forename: Steven L
authors.initials: SL
authors.lastname: Britton
authors.affiliation: Department of Physical Therapy, University of Florida , Gainesville, Florida 32610, United States.
authors.forename: Russ T
authors.initials: RT
authors.lastname: Hepple
authors.affiliation: Department of Biochemistry & Molecular Biology, Faculty of Medicine, University of Calgary , Calgary, Alberta T2N1N4, Canada.|Faculty of Kinesiology, University of Calgary , Calgary, Alberta T2N1N4, Canada.
authors.forename: Jane
authors.identifier: 0000-0002-1224-979X
authors.initials: J
authors.lastname: Shearer
mesh_terms: D000375:Aging / Q000235:genetics* / Q000378:metabolism; D000818:Animals; D009682:Magnetic Resonance Spectroscopy; D055442:Metabolome / Q000235:genetics*; D055432:Metabolomics; D009206:Myocardium / Q000378:metabolism; D010101:Oxygen Consumption / Q000235:genetics*; D051381:Rats; D012307:Risk Factors; D012420:Running
keywords: aerobic capacity; aging; metabolism; metabolomics; nuclear magnetic resonance