title: Chromosome Preparation for Acute Lymphoblastic Leukemia.
abstract: The chromosome abnormalities observed in acute lymphoblastic leukemia have been demonstrated to contribute to patient management and treatment stratification. This chapter provides a basic protocol for the procurement, culture, harvest, and slide preparation of bone marrow aspirate and unstimulated peripheral blood specimens.
authors.affiliation: Department of Paediatric Laboratory Medicine, The Hospital for Sick Children, 555 University Avenue, Toronto, ON, Canada, M5G 1X8. mary.shago@sickkids.ca.|Department of Laboratory Medicine and Pathobiology, University of Toronto, Toronto, ON, Canada, M5S 1A1. mary.shago@sickkids.ca.
authors.forename: Mary
authors.initials: M
authors.lastname: Shago
mesh_terms: D001707:Biopsy, Needle; D001853:Bone Marrow / Q000473:pathology; D002452:Cell Count; D002478:Cells, Cultured; D002869:Chromosome Aberrations; D002871:Chromosome Banding; D020732:Cytogenetic Analysis / Q000379:methods; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods; D007621:Karyotyping / Q000379:methods; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000175:diagnosis* / Q000235:genetics*
keywords: Acute lymphoblastic leukemia; Cell culture; Cytogenetics; Fluorescence in situ hybridization (FISH); Slide preparation