title: Thorium impact on tobacco root transcriptome.
abstract: Thorium is natural actinide metal with potential use in nuclear energetics. Contamination by thorium, originated from mining activities or spills, represents environmental risk due to its radioactivity and chemical toxicity. A promising approach for cleaning of contaminated areas is phytoremediation, which need to be based, however, on detail understanding of the thorium effects on plants. In this study we investigated transcriptomic response of tobacco roots exposed to 200μM thorium for one week. Thorium application resulted in up-regulation of 152 and down-regulation of 100 genes (p-value <0.01, fold change ≥2). The stimulated genes were involved in components of jasmonic acid and salicylic acid signaling pathways and various abiotic (e.g. oxidative stress) and biotic stress (e.g. pathogens, wounding) responsive genes. Further, up-regulation of phosphate starvation genes and down-regulation of genes involved in phytic acid biosynthesis indicated that thorium disturbed phosphate uptake or signaling. Also expression of iron responsive genes was influenced. Negative regulation of several aquaporins indicated disturbance of water homeostasis. Genes potentially involved in thorium transport could be zinc-induced facilitator ZIF2, plant cadmium resistance PCR2, and ABC transporter ABCG40. This study provides the first insight at the processes in plants exposed to thorium.
authors.affiliation: Laboratory of Plant Biotechnologies, Institute of Experimental Botany AS CR, v.v.i., Rozvojová 263, 165 02 Prague 6, Lysolaje, Czechia; Faculty of Environmental Sciences, Czech University of Life Sciences Prague, Kamýcká 129, Praha 6, Suchdol, 165 21, Czechia.
authors.forename: Kateřina
authors.initials: K
authors.lastname: Mazari
authors.affiliation: Laboratory of Plant Biotechnologies, Institute of Experimental Botany AS CR, v.v.i., Rozvojová 263, 165 02 Prague 6, Lysolaje, Czechia.
authors.forename: Přemysl
authors.initials: P
authors.lastname: Landa
authors.affiliation: Laboratory of Hormonal Regulations in Plants, Institute of Experimental Botany AS CR, v.v.i., Rozvojová 263, 165 02 Prague 6, Lysolaje, Czechia; Department of Experimental Plant Biology, Faculty of Science, Charles University in Prague, Viničná 5, 128 44 Prague 2, Czechia.
authors.forename: Sylva
authors.initials: S
authors.lastname: Přerostová
authors.affiliation: Laboratory of Hormonal Regulations in Plants, Institute of Experimental Botany AS CR, v.v.i., Rozvojová 263, 165 02 Prague 6, Lysolaje, Czechia.
authors.forename: Karel
authors.initials: K
authors.lastname: Müller
authors.affiliation: Laboratory of Hormonal Regulations in Plants, Institute of Experimental Botany AS CR, v.v.i., Rozvojová 263, 165 02 Prague 6, Lysolaje, Czechia.
authors.forename: Radomíra
authors.initials: R
authors.lastname: Vaňková
authors.affiliation: Laboratory of Plant Biotechnologies, Institute of Experimental Botany AS CR, v.v.i., Rozvojová 263, 165 02 Prague 6, Lysolaje, Czechia.
authors.forename: Petr
authors.initials: P
authors.lastname: Soudek
authors.affiliation: Laboratory of Plant Biotechnologies, Institute of Experimental Botany AS CR, v.v.i., Rozvojová 263, 165 02 Prague 6, Lysolaje, Czechia. Electronic address: vanek@ueb.cas.cz.
authors.forename: Tomáš
authors.initials: T
authors.lastname: Vaněk
mesh_terms: D002104:Cadmium / Q000378:metabolism; D003517:Cyclopentanes / Q000378:metabolism; D015536:Down-Regulation; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D007501:Iron / Q000737:chemistry; D018384:Oxidative Stress; D054883:Oxylipins / Q000378:metabolism; D010710:Phosphates / Q000737:chemistry; D018515:Plant Leaves / Q000378:metabolism; D018517:Plant Roots / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D020156:Salicylic Acid / Q000378:metabolism; D013312:Stress, Physiological / Q000187:drug effects; D013910:Thorium / Q000494:pharmacology*; D014026:Nicotiana / Q000187:drug effects*; D059467:Transcriptome; D015854:Up-Regulation
chemical_list: D003517:Cyclopentanes; D054883:Oxylipins; D010710:Phosphates; D017382:Reactive Oxygen Species; D002104:Cadmium; D013910:Thorium; C011006:jasmonic acid; D007501:Iron; D020156:Salicylic Acid
keywords: Gene expression; Microarray; Nicotiana tabacum; Thorium; Toxicity