title: LptD is a promising vaccine antigen and potential immunotherapeutic target for protection against Vibrio species infection.
abstract: Outer membrane proteins (OMPs) are unique to Gram-negative bacteria. Several features, including surface exposure, conservation among strains and ability to induce immune responses, make OMPs attractive targets for using as vaccine antigens and immunotherapeutics. LptD is an essential OMP that mediates the final transport of lipopolysaccharide (LPS) to outer leaflet. The protein in Vibrio parahaemolyticus was identified to have immunogenicity in our previous report. In this study, broad distribution, high conservation and similar surface-epitopes of LptD were found among the major Vibrio species. LptD was further revealed to be associated with immune responses, and it has a strong ability to stimulate antibody response. More importantly, it conferred 100% immune protection against lethal challenge by V. parahaemolyticus in mice when the mice were vaccinated with LptD, and this finding was consistent with the observation of efficient clearance of bacteria in vaccination mice. Strikingly, targeting of bacteria by the LptD antibody caused significant decreases in both the growth and LPS level and an increase in susceptibility to hydrophobic antibiotics. These findings were consistent with those previously obtained in lptD-deletion bacteria. These data demonstrated LptD is a promising vaccine antigens and a potential target for antibody-based therapy to protect against Vibrio infections.
authors.affiliation: School of Life Sciences, Zhejiang Sci-Tech University, Hangzhou 310018, China.|Zhejiang Provincial Key Laboratory of Silkworm Bioreactor and Biomedicine, Hangzhou 310018, China.
authors.forename: Zhenzhong
authors.initials: Z
authors.lastname: Zha
authors.affiliation: School of Life Sciences, Zhejiang Sci-Tech University, Hangzhou 310018, China.|Zhejiang Provincial Key Laboratory of Silkworm Bioreactor and Biomedicine, Hangzhou 310018, China.
authors.forename: Chuchu
authors.initials: C
authors.lastname: Li
authors.affiliation: School of Life Sciences, Zhejiang Sci-Tech University, Hangzhou 310018, China.|Zhejiang Provincial Key Laboratory of Silkworm Bioreactor and Biomedicine, Hangzhou 310018, China.
authors.forename: Weiyan
authors.initials: W
authors.lastname: Li
authors.affiliation: School of Life Sciences, Zhejiang Sci-Tech University, Hangzhou 310018, China.|Zhejiang Provincial Key Laboratory of Silkworm Bioreactor and Biomedicine, Hangzhou 310018, China.
authors.forename: Zhicang
authors.initials: Z
authors.lastname: Ye
authors.affiliation: School of Life Sciences, Zhejiang Sci-Tech University, Hangzhou 310018, China.|Zhejiang Provincial Key Laboratory of Silkworm Bioreactor and Biomedicine, Hangzhou 310018, China.
authors.forename: Jianyi
authors.initials: J
authors.lastname: Pan
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D000907:Antibodies, Bacterial / Q000276:immunology; D000942:Antigens, Bacterial / Q000737:chemistry / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification; D001425:Bacterial Outer Membrane Proteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification; D001428:Bacterial Vaccines / Q000276:immunology*; D003001:Cloning, Molecular; D019295:Computational Biology / Q000379:methods; D000939:Epitopes / Q000276:immunology; D015870:Gene Expression; D006801:Humans; D051379:Mice; D008826:Microbial Sensitivity Tests; D008958:Models, Molecular; D010802:Phylogeny; D011487:Protein Conformation; D013329:Structure-Activity Relationship; D014733:Vibrio / Q000145:classification / Q000187:drug effects / Q000235:genetics / Q000276:immunology*; D014735:Vibrio Infections / Q000276:immunology* / Q000517:prevention & control / Q000628:therapy
chemical_list: D000900:Anti-Bacterial Agents; D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins; D001428:Bacterial Vaccines; D000939:Epitopes