title: Ontogeny, aging, and gender-related changes in hepatic multidrug resistant protein genes in rats.
abstract: Multidrug resistance proteins (Mrps) are efflux transporters playing important roles in endogenous substances and xenobiotics transport out of the liver. Children, elderly, gender and physio-pathological conditions could influence their expression and result in changes in drug disposition.
  
  
  OBJECTIVE
  This study was aimed to examine the development-, aging-, and sex-dependent changes in Mrp1-4 and ATP-binding cassette sub-family G member 2 (Abcg2) gene expressions in livers of rats.
  
  
  METHODS
  The livers from male and female SD rats at development (-2, 1,7,14,21,28,35, and 60d) and aging (28, 60, 180 and 540d) were collected and total RNA was isolated, purified and subjected to real-time RT-PCR analysis.
  
  
  RESULTS
  Results showed that expression of Mrp1 was low, while Abcg2 and Mrp2 were the high in the liver. Mrp1 expression decreased with maturity but remained constant to 540d, while Mrp3 and 4 increased with liver development, reached the peak with maturity at 35-60days of age, and slightly reduced with aging. Mrp2 and Abcg2 were high at 7days of age and maintained at relative high levels till maturity, while Abcg2 was reduced during aging. Females had higher Mrp3 and Abcg2 mRNA expression than male rats, while male rats had higher Mrp2 and Mrp4 mRNA expression.
  
  
  CONCLUSIONS
  The expression of hepatic Mrp1-4 and Abcg2 mRNA during development, aging in male and female rats was characterized, which could be fundamental to our understanding of age- and sex-associated variations in drug disposition in children, elderly, and women.
authors.affiliation: Key Laboratory of Pharmacology of Ministry of Education, Zunyi Medical College, Zunyi, China; Department of Clinical Pharmacology, Xiangya Hospital, Institute of Clinical Pharmacology, Central South University, Hunan Key Laboratory of Pharmacogenetics, Changsha, China.
authors.forename: Qiong-Ni
authors.initials: QN
authors.lastname: Zhu
authors.affiliation: Key Laboratory of Pharmacology of Ministry of Education, Zunyi Medical College, Zunyi, China.
authors.forename: Wei-Yu
authors.initials: WY
authors.lastname: Hou
authors.affiliation: Key Laboratory of Pharmacology of Ministry of Education, Zunyi Medical College, Zunyi, China.
authors.forename: Shang-Fu
authors.initials: SF
authors.lastname: Xu
authors.affiliation: Key Laboratory of Pharmacology of Ministry of Education, Zunyi Medical College, Zunyi, China.
authors.forename: Yuan-Fu
authors.initials: YF
authors.lastname: Lu
authors.affiliation: Key Laboratory of Pharmacology of Ministry of Education, Zunyi Medical College, Zunyi, China. Electronic address: Jieliu@zmc.edu.cn.
authors.forename: Jie
authors.initials: J
authors.lastname: Liu
mesh_terms: D000070997:ATP Binding Cassette Transporter, Subfamily G, Member 2 / Q000378:metabolism; D018528:ATP-Binding Cassette Transporters / Q000378:metabolism; D000375:Aging; D000818:Animals; D005260:Female; D005786:Gene Expression Regulation; D008099:Liver / Q000378:metabolism; D008297:Male; D027425:ATP-Binding Cassette, Sub-Family C Proteins / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D060888:Real-Time Polymerase Chain Reaction; D015262:Xenobiotics
chemical_list: D000070997:ATP Binding Cassette Transporter, Subfamily G, Member 2; D018528:ATP-Binding Cassette Transporters; D027425:ATP-Binding Cassette, Sub-Family C Proteins; D015262:Xenobiotics; C423013:Abcc2 protein, rat; C489542:Abcc4 protein, rat; C489249:Abcg2 protein, rat; C114462:multidrug resistance-associated protein 3; C421109:multidrug resistance-associated protein 1
keywords: Abcg2; Aging; Development; Mrp transporters; Rat liver; Sex-difference