title: Interaction between Saccharomyces cerevisiae Mitochondrial DNA-Binding Protein Abf2p and Cce1p Resolvase.
abstract: Mitochondrial DNA is susceptible to the action of reactive oxygen species generated by the reactions of oxidative phosphorylation. Homologous recombination is one of the mechanisms providing integrity of the mitochondrial genome. Some proteins that take part in this process in budding yeast mitochondria have been identified. These include Abf2p, the major protein of the mt-nucleoid that specifically binds cruciform DNA, and Cce1p - Holliday junction resolvase. Here we show that Abf2p does not significantly affect either binding of Cce1p to branched DNA or rate and specificity of Holliday junction resolution. These data suggest the existence of an alternative homologous recombination pathway in yeast mitochondria.
authors.affiliation: Lomonosov Moscow State University, Faculty of Biology, Moscow, 119991, Russia. krolick@yandex.ru.
authors.forename: E O
authors.initials: EO
authors.lastname: Samoilova
authors.forename: I A
authors.initials: IA
authors.lastname: Krasheninnikov
authors.forename: S A
authors.initials: SA
authors.lastname: Levitskii
mesh_terms: D045566:DNA, Cruciform / Q000235:genetics / Q000378:metabolism; D004271:DNA, Fungal / Q000235:genetics / Q000378:metabolism*; D004272:DNA, Mitochondrial / Q000235:genetics / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D045565:Holliday Junction Resolvases / Q000235:genetics / Q000378:metabolism*; D059765:Homologous Recombination / Q000502:physiology*; D024101:Mitochondrial Proteins / Q000235:genetics / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism*; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*
chemical_list: C072843:ABF2 protein, S cerevisiae; D045566:DNA, Cruciform; D004271:DNA, Fungal; D004272:DNA, Mitochondrial; D004268:DNA-Binding Proteins; D024101:Mitochondrial Proteins; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors; C473486:CCE1 protein, S cerevisiae; D045565:Holliday Junction Resolvases