title: Crystallographic structure of the turbine C-ring from spinach chloroplast F-ATP synthase.
abstract: In eukaryotic and prokaryotic cells, F-ATP synthases provide energy through the synthesis of ATP. The chloroplast F-ATP synthase (CF1FO-ATP synthase) of plants is integrated into the thylakoid membrane via its FO-domain subunits a, b, b' and c Subunit c with a stoichiometry of 14 and subunit a form the gate for H+-pumping, enabling the coupling of electrochemical energy with ATP synthesis in the F1sector.Here we report the crystallization and structure determination of the c14-ring of subunit c of the CF1FO-ATP synthase from spinach chloroplasts. The crystals belonged to space group C2, with unit-cell parameters a=144.420, b=99.295, c=123.51 Å, and β=104.34° and diffracted to 4.5 Å resolution. Each c-ring contains 14 monomers in the asymmetric unit. The length of the c-ring is 60.32 Å, with an outer ring diameter 52.30 Å and an inner ring width of 40 Å.
authors.affiliation: Nanyang Technological University, School of Biological Sciences, 60 Nanyang Drive, Singapore 637551, Republic of Singapore.
authors.forename: Asha Manikkoth
authors.initials: AM
authors.lastname: Balakrishna
authors.affiliation: Physikalische Biochemie, Fachbereich Chemie, Technische Universität Darmstadt, Alarich-Weiss-Str.4, D-64287 Darmstadt, Germany.
authors.forename: Holger
authors.initials: H
authors.lastname: Seelert
authors.affiliation: Physikalische Biochemie, Fachbereich Chemie, Technische Universität Darmstadt, Alarich-Weiss-Str.4, D-64287 Darmstadt, Germany.
authors.forename: Sven-Hendric
authors.initials: SH
authors.lastname: Marx
authors.affiliation: Physikalische Biochemie, Fachbereich Chemie, Technische Universität Darmstadt, Alarich-Weiss-Str.4, D-64287 Darmstadt, Germany.
authors.forename: Norbert A
authors.initials: NA
authors.lastname: Dencher
authors.affiliation: Nanyang Technological University, School of Biological Sciences, 60 Nanyang Drive, Singapore 637551, Republic of Singapore ggrueber@ntu.edu.sg.
authors.forename: Gerhard
authors.initials: G
authors.lastname: Grüber
mesh_terms: D060365:Chloroplast Proteins / Q000737:chemistry*; D018360:Crystallography, X-Ray; D025261:Mitochondrial Proton-Translocating ATPases / Q000737:chemistry*; D020836:Protein Structure, Quaternary; D018724:Spinacia oleracea / Q000201:enzymology*; D020524:Thylakoids / Q000201:enzymology*
chemical_list: D060365:Chloroplast Proteins; C474451:F1F0-ATP synthase; D025261:Mitochondrial Proton-Translocating ATPases
keywords: CF1FO-ATP synthase; F-ATP synthase; c-rotor; crystallization; spinach chloroplast