title: Strategies for the photo-control of endogenous protein activity.
abstract: Photo-controlled or 'optogenetic' effectors interfacing with endogenous protein machinery allow the roles of endogenous proteins to be probed. There are two main approaches being used to develop optogenetic effectors: (i) caging strategies using photo-controlled conformational changes, and (ii) protein relocalization strategies using photo-controlled protein-protein interactions. Numerous specific examples of these approaches have been reported and efforts to develop general methods for photo-control of endogenous proteins are a current focus. The development of improved screening and selection methods for photo-switchable proteins would advance the field.
authors.affiliation: Department of Chemistry, University of Toronto, Toronto, Canada; Molecular Biotechnology, University of Potsdam, Potsdam, Germany.
authors.forename: Katherine E
authors.initials: KE
authors.lastname: Brechun
authors.affiliation: Molecular Biotechnology, University of Potsdam, Potsdam, Germany.
authors.forename: Katja M
authors.initials: KM
authors.lastname: Arndt
authors.affiliation: Department of Chemistry, University of Toronto, Toronto, Canada. Electronic address: awoolley@chem.utoronto.ca.
authors.forename: G Andrew
authors.initials: GA
authors.lastname: Woolley
mesh_terms: D042541:Intracellular Space / Q000378:metabolism; D062308:Optogenetics / Q000379:methods*; D021381:Protein Transport / Q000235:genetics / Q000528:radiation effects; D011506:Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D059748:Proteolysis / Q000528:radiation effects
chemical_list: D011506:Proteins