title: Co-metabolism of substrates by Bacillus thuringiensis regulates polyhydroxyalkanoate co-polymer composition.
abstract: Polyhydroxyalkanoate (PHA) production by Bacillus thuringiensis EGU45 was studied by co-metabolism of crude glycerol (CG) (1%, v/v), glucose (0.05-0.5%, w/v) and propionic acid (0.05-0.5%, v/v) under batch (shake flask) culture conditions. Glycerol+PA combination resulted in 15-100mg/L PHA co-polymers with a HV content of 33-81mol%. The addition of NH4Cl (0.5%, w/v) to CG+PA enhanced PHA production by 1.55-fold, with a HV content of 58-70mol%. The time period of incubation of PA to the feed: CG+glucose was optimized to be 3h after initiation of fermentation. The PHA contents were found to be stable at 1900-2050mg/L up scaling from 0.4 to 2.0L feed material. Biochemical characterization through GC-MS of PHA co-polymer revealed the presence of 3-hydroxydecanoate (3-HDD), 3-hydroxyoctadecanoate (3HOD), 3-hydroxyhexadecanoate (3HHD).
authors.affiliation: Microbial Biotechnology and Genomics, CSIR - Institute of Genomics and Integrative Biology (IGIB), Delhi University Campus, Mall Road, Delhi 110007, India; Academy of Scientific & Innovative Research (AcSIR), 2, Rafi Marg, Anusandhan Bhawan, New Delhi 110001, India. Electronic address: subhasreeray141@gmail.com.
authors.forename: Subhasree
authors.initials: S
authors.lastname: Ray
authors.affiliation: Microbial Biotechnology and Genomics, CSIR - Institute of Genomics and Integrative Biology (IGIB), Delhi University Campus, Mall Road, Delhi 110007, India; Academy of Scientific & Innovative Research (AcSIR), 2, Rafi Marg, Anusandhan Bhawan, New Delhi 110001, India.
authors.forename: Vipin Chandra
authors.initials: VC
authors.lastname: Kalia
mesh_terms: D001413:Bacillus thuringiensis / Q000378:metabolism*; D003470:Culture Media / Q000737:chemistry; D003652:Decanoic Acids / Q000032:analysis; D005285:Fermentation; D005990:Glycerol / Q000378:metabolism; D007218:Industrial Microbiology / Q000379:methods; D011091:Polyesters / Q000378:metabolism; D054813:Polyhydroxyalkanoates / Q000096:biosynthesis* / Q000737:chemistry* / Q000378:metabolism; D013228:Stearates / Q000032:analysis
chemical_list: D003470:Culture Media; D003652:Decanoic Acids; D011091:Polyesters; D054813:Polyhydroxyalkanoates; D013228:Stearates; C024708:3-hydroxyoctadecanoate; C017552:myrmicacin; D005990:Glycerol
keywords: Bacillus; Co-polymer; Crude glycerol; Polyhydroxyalkanoate; Precursor substrate