title: Preservation of Malassezia pachydermatis using skimmed milk as cryoprotective agent.
abstract: This study evaluated the effect of skimmed milk as a cryoprotectant for the maintenance and long-term preservation of 70 Malassezia pachydermatis strains. An initial suspension of each strain was prepared in sterile distilled water with skimmed milk. The most effective method was cryopreservation that ensured 100% viability for 2years.
authors.affiliation: University Institute of Animal Health (IUSA), University of Las Palmas de Gran Canaria, 35413 Arucas, Las Palmas, Spain.
authors.forename: F A
authors.initials: FA
authors.lastname: Silva Sergent
authors.affiliation: University Institute of Animal Health (IUSA), University of Las Palmas de Gran Canaria, 35413 Arucas, Las Palmas, Spain. Electronic address: bego.acosta@ulpgc.es.
authors.forename: B
authors.initials: B
authors.lastname: Acosta-Hernández
authors.affiliation: University Institute of Animal Health (IUSA), University of Las Palmas de Gran Canaria, 35413 Arucas, Las Palmas, Spain.
authors.forename: J
authors.initials: J
authors.lastname: Raduán Jaber
authors.affiliation: University Institute of Animal Health (IUSA), University of Las Palmas de Gran Canaria, 35413 Arucas, Las Palmas, Spain.
authors.forename: I
authors.initials: I
authors.lastname: Rosario Medina
authors.affiliation: University Institute of Animal Health (IUSA), University of Las Palmas de Gran Canaria, 35413 Arucas, Las Palmas, Spain.
authors.forename: O
authors.initials: O
authors.lastname: Ferrer Quintana
authors.affiliation: University Institute of Animal Health (IUSA), University of Las Palmas de Gran Canaria, 35413 Arucas, Las Palmas, Spain.
authors.forename: F
authors.initials: F
authors.lastname: Real Valcárcel
authors.affiliation: University Institute of Animal Health (IUSA), University of Las Palmas de Gran Canaria, 35413 Arucas, Las Palmas, Spain.
authors.forename: D
authors.initials: D
authors.lastname: Padilla Castillo
authors.affiliation: University Institute of Animal Health (IUSA), University of Las Palmas de Gran Canaria, 35413 Arucas, Las Palmas, Spain.
authors.forename: S
authors.initials: S
authors.lastname: Déniz Suárez
mesh_terms: D000818:Animals; D015925:Cryopreservation / Q000379:methods*; D003451:Cryoprotective Agents / Q000737:chemistry*; D008290:Malassezia / Q000302:isolation & purification*; D050296:Microbial Viability; D008892:Milk / Q000737:chemistry*; D013997:Time Factors
chemical_list: D003451:Cryoprotective Agents
keywords: Cryoprotectant; Maintenance; Malassezia pachydermatis; Preservation