title: Histone chaperone activity of Arabidopsis thaliana NRP1 is blocked by cytochrome c.
abstract: Higher-order plants and mammals use similar mechanisms to repair and tolerate oxidative DNA damage. Most studies on the DNA repair process have focused on yeast and mammals, in which histone chaperone-mediated nucleosome disassembly/reassembly is essential for DNA to be accessible to repair machinery. However, little is known about the specific role and modulation of histone chaperones in the context of DNA damage in plants. Here, the histone chaperone NRP1, which is closely related to human SET/TAF-Iβ, was found to exhibit nucleosome assembly activity in vitro and to accumulate in the chromatin of Arabidopsis thaliana after DNA breaks. In addition, this work establishes that NRP1 binds to cytochrome c, thereby preventing the former from binding to histones. Since NRP1 interacts with cytochrome c at its earmuff domain, that is, its histone-binding domain, cytochrome c thus competes with core histones and hampers the activity of NRP1 as a histone chaperone. Altogether, the results obtained indicate that the underlying molecular mechanisms in nucleosome disassembly/reassembly are highly conserved throughout evolution, as inferred from the similar inhibition of plant NRP1 and human SET/TAF-Iβ by cytochrome c during DNA damage response.
authors.affiliation: Institute for Chemical Research (IIQ), Isla de la Cartuja Scientific Research Centre (cicCartuja), University of Seville-Spanish National Research Council (CSIC), Avda. Américo Vespucio 49, 41092 Sevilla, Spain.
authors.forename: Katiuska
authors.initials: K
authors.lastname: González-Arzola
authors.affiliation: Institute for Chemical Research (IIQ), Isla de la Cartuja Scientific Research Centre (cicCartuja), University of Seville-Spanish National Research Council (CSIC), Avda. Américo Vespucio 49, 41092 Sevilla, Spain.
authors.forename: Antonio
authors.initials: A
authors.lastname: Díaz-Quintana
authors.affiliation: Institute for Chemical Research (IIQ), Isla de la Cartuja Scientific Research Centre (cicCartuja), University of Seville-Spanish National Research Council (CSIC), Avda. Américo Vespucio 49, 41092 Sevilla, Spain.
authors.forename: Francisco
authors.initials: F
authors.lastname: Rivero-Rodríguez
authors.affiliation: Institute for Biocomputation and Physics of Complex Systems (BIFI), Joint Unit Institute of Physical Chemistry Rocasolano (IQFR)-BIFI-Spanish National Research Council (CSIC), University of Zaragoza, Mariano Esquillor s/n, 50018 Zaragoza, Spain.|Department of Biochemistry and Molecular and Cellular Biology, University of Zaragoza, Pedro Cerbuna 12, 50009 Zaragoza (Spain); and Aragon Agency for Research and Development (ARAID), Regional Government of Aragon, Maria de Luna 11, 50018 Zaragoza, Spain.
authors.forename: Adrián
authors.initials: A
authors.lastname: Velázquez-Campoy
authors.affiliation: Institute for Chemical Research (IIQ), Isla de la Cartuja Scientific Research Centre (cicCartuja), University of Seville-Spanish National Research Council (CSIC), Avda. Américo Vespucio 49, 41092 Sevilla, Spain.
authors.forename: Miguel A
authors.initials: MA
authors.lastname: De la Rosa
authors.affiliation: Institute for Chemical Research (IIQ), Isla de la Cartuja Scientific Research Centre (cicCartuja), University of Seville-Spanish National Research Council (CSIC), Avda. Américo Vespucio 49, 41092 Sevilla, Spain.
authors.forename: Irene
authors.initials: I
authors.lastname: Díaz-Moreno
mesh_terms: D017360:Arabidopsis; D029681:Arabidopsis Proteins / Q000737:chemistry / Q000378:metabolism*; D002478:Cells, Cultured; D002843:Chromatin / Q000235:genetics / Q000378:metabolism; D045304:Cytochromes c / Q000737:chemistry / Q000378:metabolism*; D004249:DNA Damage; D004268:DNA-Binding Proteins; D056488:Histone Chaperones / Q000737:chemistry / Q000378:metabolism*; D006657:Histones / Q000378:metabolism; D006801:Humans; D008958:Models, Molecular; D009707:Nucleosomes / Q000378:metabolism; D059828:Plant Cells; D011485:Protein Binding; D011487:Protein Conformation; D054730:Protein Interaction Domains and Motifs; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013816:Thermodynamics; D014157:Transcription Factors / Q000378:metabolism
chemical_list: D029681:Arabidopsis Proteins; D002843:Chromatin; D004268:DNA-Binding Proteins; D056488:Histone Chaperones; D006657:Histones; D009707:Nucleosomes; C513473:SET protein, human; D014157:Transcription Factors; C551996:nodulin-related protein 1, Arabidopsis; D045304:Cytochromes c