title: [Association between IL-6-572 C/G polymorphism and breast cancer susceptibility].
abstract: Objective To explore the associations between breast cancer and three single nucleotide polymorphisms (-597 G/A, -572 C/G and -174 G/C) of interleukin-6 (IL-6) gene promoter. Methods The study enrolled 136 breast cancer cases and 150 healthy female controls. The single nucleotide polymorphisms (SNPs) of IL-6 were identified by Sanger method of DNA sequencing, and serum IL-6 levels were measured with electrochemiluminescence immunoassay. Chi-squared test was utilized to compare frequency of a given SNP between the two groups, and t-test was employed to compare serum IL-6 levels in different groups. Results Compared with healthy controls, the serum IL-6 levels were significantly elevated in the patients with breast cancer. The genotype frequency of -572 C/G was significantly higher in breast cancer group than in the control group (OR=1.841, 95%CI: 1.115-3.040, P=0.017). There was no significant difference of serum IL-6 level in the three genotypes of -572 C/G, in both the breast cancer cases and the healthy controls. Conclusion The serum level of IL-6 significantly increases in the patients with breast cancer. The -572 C/G polymorphism and the serum level of IL-6 may play a role in the development of breast cancer.
authors.affiliation: Department of Clinical Laboratory, Quanzhou First Hospital, Fujian Medical University, Quanzhou 362000, China. *Corresponding author, E-mail: 554882707@qq.com.
authors.forename: Zhishan
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: Department of Clinical Laboratory, Quanzhou First Hospital, Fujian Medical University, Quanzhou 362000, China.
authors.forename: Zixuan
authors.initials: Z
authors.lastname: Chen
authors.affiliation: Department of Breast Surgery, Quanzhou First Hospital, Fujian Medical University, Quanzhou 362000, China.
authors.forename: Debo
authors.initials: D
authors.lastname: Chen
authors.affiliation: Department of Clinical Laboratory, Quanzhou Central Blood Station, Quanzhou 362000, China.
authors.forename: Yisheng
authors.initials: Y
authors.lastname: Lin
authors.affiliation: Department of Clinical Laboratory, Quanzhou First Hospital, Fujian Medical University, Quanzhou 362000, China.
authors.forename: Yancheng
authors.initials: Y
authors.lastname: Jiang
authors.affiliation: Department of Breast Surgery, Quanzhou First Hospital, Fujian Medical University, Quanzhou 362000, China.
authors.forename: Qinglan
authors.initials: Q
authors.lastname: Wang
mesh_terms: D001943:Breast Neoplasms / Q000235:genetics*; D016009:Chi-Square Distribution; D005260:Female; D005787:Gene Frequency / Q000235:genetics; D005838:Genotype; D006801:Humans; D015850:Interleukin-6 / Q000097:blood / Q000235:genetics*; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D011401:Promoter Regions, Genetic / Q000235:genetics
chemical_list: D015850:Interleukin-6