title: Forsythia suspensa Suppresses House Dust Mite Extract-Induced Atopic Dermatitis in NC/Nga Mice.
abstract: Forsythia suspensa (F. suspensa) is a traditional medicine for treatment of inflammation. In this study, we evaluated the therapeutic effects of an ethanol extract from F. suspensa fruits on atopic dermatitis both in vivo and in vitro. We investigated the inhibitory effects of F. suspensa extract on the development of atopic dermatitis-like skin lesions in an NC/Nga mouse model exposed to Dermatophagoides farinae crude extract. Topical application of F. suspensa extract to the mice attenuated the atopic dermatitis symptoms, including increased dermatitis severity score, ear thickness, infiltration of inflammatory cells in the skin lesions, serum levels of IgE, TNF-α, and histamine, and expression of chemokines, cytokines, and adhesion molecules in ear tissue. In addition, F. suspensa extract inhibited the production of chemokines in TNF-α/IFN-γ-activated human keratinocytes. High-performance liquid chromatography analysis of FSE revealed the presence of four chemical constituents (forsythiaside, phillyrin, pinoresinol, and phylligenin). These compounds inhibited the production of chemokines in TNF-α/IFN-γ-activated human keratinocytes. These results suggest that the F. suspensa might be a useful candidate for treating allergic skin inflammatory disorders.
authors.affiliation: Mibyeong Research Center, Korea Institute of Oriental Medicine, Daejeon, Republic of Korea.
authors.forename: Yoon-Young
authors.initials: YY
authors.lastname: Sung
authors.affiliation: College of Pharmacy, Graduate School of Pharmaceutical Sciences, Ewha Womans University, Seoul, Republic of Korea.
authors.forename: Taesook
authors.initials: T
authors.lastname: Yoon
authors.affiliation: Mibyeong Research Center, Korea Institute of Oriental Medicine, Daejeon, Republic of Korea.
authors.forename: Seol
authors.initials: S
authors.lastname: Jang
authors.affiliation: Mibyeong Research Center, Korea Institute of Oriental Medicine, Daejeon, Republic of Korea.
authors.forename: Ho Kyoung
authors.initials: HK
authors.lastname: Kim
mesh_terms: D000485:Allergens; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000737:chemistry / Q000302:isolation & purification / Q000627:therapeutic use*; D002460:Cell Line; D016207:Cytokines / Q000032:analysis / Q000276:immunology; D003876:Dermatitis, Atopic / Q000188:drug therapy* / Q000209:etiology / Q000276:immunology / Q000473:pathology; D004195:Disease Models, Animal; D031657:Forsythia / Q000737:chemistry*; D006801:Humans; D015603:Keratinocytes / Q000187:drug effects / Q000276:immunology / Q000473:pathology; D008297:Male; D051379:Mice; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000627:therapeutic use*; D039981:Pyroglyphidae / Q000276:immunology*; D012867:Skin / Q000187:drug effects* / Q000276:immunology / Q000473:pathology
chemical_list: D000485:Allergens; D000893:Anti-Inflammatory Agents; D016207:Cytokines; D010936:Plant Extracts