title: Characteristics of NH4+and NO3-fluxes in tea (Camellia sinensis) roots measured by scanning ion-selective electrode technique.
abstract: As a vital beverage crop, tea has been extensively planted in tropical and subtropical regions. Nitrogen (N) levels and forms are closely related to tea quality. Based on different N levels and forms, we studied changes in NO3-and NH4+fluxes in tea roots utilizing scanning ion-selective electrode technique. Our results showed that under both single and mixed N forms, influx rates of NO3-were much lower than those of NH4+, suggesting a preference for NH4+in tea. With the increase in N concentration, the influx rate of NO3-increased more than that of NH4+. The NH4+influx rates in a solution without NO3-were much higher than those in a solution with NO3-, while the NO3-influx rates in a solution without NH4+were much lower than those in a solution with NH4+. We concluded that (1) tea roots showed a preference for NH4+, (2) presence of NO3-had a negative effect on NH4+influx, and (3) NH4+had a positive effect on NO3-influx. Our findings not only may help advance hydroponic tea experiments but also may be used to develop efficient fertilization protocols for soil-grown tea in the future.
authors.affiliation: National Center for Tea Improvement, Tea Research Institute, Chinese Academy of Agricultural Sciences; Key Laboratory of Tea Plant Biology and Resources Utilization, Ministry of Agriculture, Hangzhou 310008, China.|State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences, Nanjing, 210008, China.
authors.forename: Li
authors.initials: L
authors.lastname: Ruan
authors.affiliation: National Center for Tea Improvement, Tea Research Institute, Chinese Academy of Agricultural Sciences; Key Laboratory of Tea Plant Biology and Resources Utilization, Ministry of Agriculture, Hangzhou 310008, China.
authors.forename: Kang
authors.initials: K
authors.lastname: Wei
authors.affiliation: National Center for Tea Improvement, Tea Research Institute, Chinese Academy of Agricultural Sciences; Key Laboratory of Tea Plant Biology and Resources Utilization, Ministry of Agriculture, Hangzhou 310008, China.
authors.forename: Liyuan
authors.initials: L
authors.lastname: Wang
authors.affiliation: National Center for Tea Improvement, Tea Research Institute, Chinese Academy of Agricultural Sciences; Key Laboratory of Tea Plant Biology and Resources Utilization, Ministry of Agriculture, Hangzhou 310008, China.
authors.forename: Hao
authors.initials: H
authors.lastname: Cheng
authors.affiliation: National Center for Tea Improvement, Tea Research Institute, Chinese Academy of Agricultural Sciences; Key Laboratory of Tea Plant Biology and Resources Utilization, Ministry of Agriculture, Hangzhou 310008, China.
authors.forename: Fen
authors.initials: F
authors.lastname: Zhang
authors.affiliation: National Center for Tea Improvement, Tea Research Institute, Chinese Academy of Agricultural Sciences; Key Laboratory of Tea Plant Biology and Resources Utilization, Ministry of Agriculture, Hangzhou 310008, China.
authors.forename: Liyun
authors.initials: L
authors.lastname: Wu
authors.affiliation: National Center for Tea Improvement, Tea Research Institute, Chinese Academy of Agricultural Sciences; Key Laboratory of Tea Plant Biology and Resources Utilization, Ministry of Agriculture, Hangzhou 310008, China.
authors.forename: Peixian
authors.initials: P
authors.lastname: Bai
authors.affiliation: National Center for Tea Improvement, Tea Research Institute, Chinese Academy of Agricultural Sciences; Key Laboratory of Tea Plant Biology and Resources Utilization, Ministry of Agriculture, Hangzhou 310008, China.
authors.forename: Chengcai
authors.initials: C
authors.lastname: Zhang
mesh_terms: D000641:Ammonia / Q000032:analysis / Q000378:metabolism*; D028241:Camellia sinensis / Q000378:metabolism*; D055664:Electrochemical Techniques; D004566:Electrodes; D006863:Hydrogen-Ion Concentration; D018527:Hydroponics / Q000379:methods; D017136:Ion Transport; D009566:Nitrates / Q000032:analysis / Q000378:metabolism*; D009584:Nitrogen / Q000032:analysis / Q000378:metabolism*; D018517:Plant Roots / Q000378:metabolism*; D012987:Soil / Q000737:chemistry; D013662:Tea / Q000737:chemistry
chemical_list: D009566:Nitrates; D012987:Soil; D013662:Tea; D000641:Ammonia; D009584:Nitrogen