title: Cytogenetic analysis of the retained products of conception after missed abortion following blastocyst transfer: a retrospective, large-scale, single-centre study.
abstract: Cytogenetic analysis of the retained products of conception (POC) is the most effective test for identifying miscarriage causes. However, there has been no large-scale study limited to blastocyst transfer. This study retrospectively reports the findings of 1030 cases in which POC analysis was performed after missed abortion following single blastocyst transfer performed at the Shinbashi Yume Clinic. We identified 19.4% as normal karyotypes and 80.6% as aneuploid. These cases broke down into: 62.3% trisomy; 7.8% double trisomy; 0.5% triple or quadruple trisomy; 1.3% monosomy 21; 3.2% monosomy X; 0.1% 47,XXY; 1.0% polyploidy; 1.0% mixed; 1.1% embryonic mosaicism; and 2.4% structural anomalies. In samples with normal karyotypes, 49.5% were female while 50.5% were male. The occurrence of trisomy and double trisomy were both significantly more frequent in the ≥38 years group than in the ≤37 years group (P < 0.01). Trisomy was significantly more frequently associated with fetal heartbeat (P < 0.01); double trisomy, polyploidy and normal karyotype were significantly more frequent with no fetal heartbeat (P < 0.01). There was no significant difference in the frequency of chromosomal abnormalities between the number of miscarriages or blastocyst quality. Thus, POC cytogenetic testing is highly valuable for ascertaining the cause of miscarriage.
authors.affiliation: Shinbashi Yume Clinic, Excel Shinbashi, 2-5-1, Shinbashi, Minato-ku, Tokyo 105-0004, Japan. Electronic address: t-segawa@yumeclinic.net.
authors.forename: Tomoya
authors.initials: T
authors.lastname: Segawa
authors.affiliation: Kato Ladies Clinic, 7-20-3, Nishi-Shinjuku, Shinjuku-ku, Tokyo 160-0023, Japan.
authors.forename: Tomoko
authors.initials: T
authors.lastname: Kuroda
authors.affiliation: Kato Ladies Clinic, 7-20-3, Nishi-Shinjuku, Shinjuku-ku, Tokyo 160-0023, Japan.
authors.forename: Keiichi
authors.initials: K
authors.lastname: Kato
authors.affiliation: Shinbashi Yume Clinic, Excel Shinbashi, 2-5-1, Shinbashi, Minato-ku, Tokyo 105-0004, Japan.
authors.forename: Masako
authors.initials: M
authors.lastname: Kuroda
authors.affiliation: Shinbashi Yume Clinic, Excel Shinbashi, 2-5-1, Shinbashi, Minato-ku, Tokyo 105-0004, Japan.
authors.forename: Kenji
authors.initials: K
authors.lastname: Omi
authors.affiliation: Shinbashi Yume Clinic, Excel Shinbashi, 2-5-1, Shinbashi, Minato-ku, Tokyo 105-0004, Japan.
authors.forename: Osamu
authors.initials: O
authors.lastname: Miyauchi
authors.affiliation: Shinbashi Yume Clinic, Excel Shinbashi, 2-5-1, Shinbashi, Minato-ku, Tokyo 105-0004, Japan.
authors.forename: Yoshiaki
authors.initials: Y
authors.lastname: Watanabe
authors.affiliation: Shinbashi Yume Clinic, Excel Shinbashi, 2-5-1, Shinbashi, Minato-ku, Tokyo 105-0004, Japan.
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Okubo
authors.affiliation: Shinbashi Yume Clinic, Excel Shinbashi, 2-5-1, Shinbashi, Minato-ku, Tokyo 105-0004, Japan.
authors.forename: Hisao
authors.initials: H
authors.lastname: Osada
authors.affiliation: Shinbashi Yume Clinic, Excel Shinbashi, 2-5-1, Shinbashi, Minato-ku, Tokyo 105-0004, Japan.
authors.forename: Shokichi
authors.initials: S
authors.lastname: Teramoto
mesh_terms: D000030:Abortion, Missed / Q000235:genetics*; D000328:Adult; D000782:Aneuploidy; D002869:Chromosome Aberrations; D020732:Cytogenetic Analysis; D004624:Embryo Transfer; D005260:Female; D005306:Fertilization; D005307:Fertilization in Vitro; D006801:Humans; D007564:Japan; D007621:Karyotyping; D008297:Male; D008875:Middle Aged; D011247:Pregnancy; D012189:Retrospective Studies; D020554:Sperm Injections, Intracytoplasmic
keywords: blastocyst; intracytoplasmic sperm injection; karyotype; missed abortion; products of conception; vitrification