title: Low-level expression of SOD1 in peripheral blood samples of patients diagnosed with primary open-angle glaucoma.
abstract: OBJECTIVE
  Glaucoma is a multifactorial disease of retinal ganglion cells, with low prognosis. For that reason, the identification of biomarkers in peripheral blood samples for diagnostics and treatment of the disease is needed. To establish mRNA expression level of several oxidative stress biomarkers reported in aqueous humor of patients with primary open-angle glaucoma (POAG).
  
  
  METHODS
  The mRNA expression levels of eight genes from 15 patients diagnosed with POAG and 11 control subjects were analyzed using an RT2Custom Profiler PCR Array.
  
  
  RESULTS
  mRNA expression level of superoxide dismutase 1 (SOD1) showed a significant downregulation in patients diagnosed with POAG in respect to control subjects. The low expression of SOD1 found in blood is consistent with the reported proteomic levels in aqueous humor.
  
  
  CONCLUSIONS
  SOD1 may be a useful biomarker in the peripheral blood of patients diagnosed with POAG.
authors.affiliation: Department of Biology, Faculty of Natural Sciences, Universidad ICESI, Calle 18 No. 122-135, Cali - Colombia.
authors.forename: Licelly
authors.initials: L
authors.lastname: Canizales
authors.affiliation: Department of Biology, Faculty of Natural & Exact Sciences, Universidad del Valle, Calle 13 No. 100-00, Cali - Colombia.
authors.forename: Laura
authors.initials: L
authors.lastname: Rodriguez
authors.affiliation: Centro Medico Farallones, Fundacion Oftalmologica del Valle, Calle 9 c 50-25 cons 400, Cali - Colombia.
authors.forename: Carlos
authors.initials: C
authors.lastname: Rivera
authors.affiliation: Ophthalmology Residency Program, Universidad del Valle & Centro Medico Imbanaco, Carrera 38 A No. 5A 100, Cali - Colombia.
authors.forename: Alexander
authors.initials: A
authors.lastname: Martinez
authors.affiliation: School of Public Health, GESP Group, Universidad del Valle, Calle 4B No. 36A-140, Cali - Colombia.
authors.forename: Fabián
authors.initials: F
authors.lastname: Mendez
authors.affiliation: Department of Biology, Faculty of Natural & Exact Sciences, Universidad del Valle, Calle 13 No. 100-00, Cali - Colombia.
authors.forename: Andres
authors.initials: A
authors.lastname: Castillo
mesh_terms: D015415:Biomarkers / Q000097:blood; D016022:Case-Control Studies; D015536:Down-Regulation; D005902:Glaucoma, Open-Angle / Q000175:diagnosis* / Q000378:metabolism; D005980:Glutathione Reductase / Q000097:blood / Q000235:genetics / Q000378:metabolism; D006801:Humans; D008875:Middle Aged; D020411:Oligonucleotide Array Sequence Analysis; D018384:Oxidative Stress; D007703:Peptidyl-Dipeptidase A / Q000097:blood / Q000235:genetics / Q000378:metabolism; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000378:metabolism; D000072105:Superoxide Dismutase-1 / Q000097:blood* / Q000235:genetics / Q000378:metabolism
chemical_list: D015415:Biomarkers; D012333:RNA, Messenger; D000072105:Superoxide Dismutase-1; D005980:Glutathione Reductase; D007703:Peptidyl-Dipeptidase A
keywords: PCR array; SOD1; antioxidant defense; biomarkers; glaucoma; mRNA; oxidative stress; trabecular meshwork