title: Complex Genetics of Cardiovascular Traits in Mice: F2-Mapping of QTLs and Their Underlying Genes.
abstract: In this chapter, we will use the example of the identification of Tnni3k as a modulator of cardiac conduction to introduce you to the use of a murine F2-generation intercross as a powerful method for the identification of novel genes relevant for cardiovascular traits. Murine F2-progeny is a genetically diverse panel of mice with differences in phenotype manifestations, e.g. cardiovascular traits such as cardiomyopathy and ECG parameters. This chapter discusses the best strategies for using F2-mice for genetic mapping. Moreover, we provide an example of the feasibility of identification of new genes modulating cardiac function utilizing the technique of mapping quantitative trait loci (QTLs) and a systems genetics integration of available genetic, gene expression, and phenotypic data.
authors.affiliation: Department of Clinical and Experimental Cardiology, Academic Medical Centre (AMC), University of Amsterdam, Meibergdreef 9, 1105AZ, Amsterdam, The Netherlands.
authors.forename: Svitlana
authors.initials: S
authors.lastname: Podliesna
authors.affiliation: Department of Clinical and Experimental Cardiology, Academic Medical Centre (AMC), University of Amsterdam, Meibergdreef 9, 1105AZ, Amsterdam, The Netherlands.
authors.forename: Connie R
authors.initials: CR
authors.lastname: Bezzina
authors.affiliation: Department of Clinical and Experimental Cardiology, Academic Medical Centre (AMC), University of Amsterdam, Meibergdreef 9, 1105AZ, Amsterdam, The Netherlands. e.m.lodder@amc.uva.nl.
authors.forename: Elisabeth M
authors.initials: EM
authors.lastname: Lodder
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D002320:Cardiovascular Physiological Phenomena / Q000235:genetics*; D002874:Chromosome Mapping; D003433:Crosses, Genetic; D030541:Databases, Genetic; D015870:Gene Expression; D056726:Genetic Association Studies; D006239:Haplotypes; D051379:Mice; D008815:Mice, Inbred Strains; D009154:Mutation; D010641:Phenotype; D040641:Quantitative Trait Loci; D019655:Quantitative Trait, Heritable
keywords: Cardiovascular traits mapping; Use case