title: QTL Mapping and Identification of Candidate Genes in DO Mice: A Use Case Model Derived from a Benzene Toxicity Experiment.
abstract: Diversity Outbred (DO) mice are a multiparental advanced generation intercross population derived from eight inbred strains which are genetically very diverse. They are maintained as an outbred population using a randomized mating design. Thus DO mice represent an ideal population to map phenotypic traits. Here, we provide a case study in which male DO mice were exposed to benzene and phenotyped for the number of micronucleated reticulocytes. We provide step-by-step R scripts for the analysis of phenotypes, genotypes, mapping of resistance gene loci and identification of candidate genes.
authors.affiliation: The Jackson Laboratory, Bar Harbor, ME, USA.
authors.forename: Dan
authors.initials: D
authors.lastname: Gatti
authors.affiliation: Center for Pharmacogenomics and Individualized Therapy, School of Pharmacy, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, 27599, USA.
authors.forename: John E
authors.initials: JE
authors.lastname: French
authors.affiliation: Department of Infection Genetics, Helmholtz Centre for Infection Research & University of Veterinary Medicine Hannover, Braunschweig, Niedersachsen, Germany. klaus.schughart@helmholtz-hzi.de.|Department of Microbiology, Immunology and Biochemistry, University of Tennessee Health Science Center, Memphis, TN, USA. klaus.schughart@helmholtz-hzi.de.
authors.forename: Klaus
authors.initials: K
authors.lastname: Schughart
mesh_terms: D000483:Alleles; D000704:Analysis of Variance; D000818:Animals; D001499:Bayes Theorem; D001554:Benzene / Q000633:toxicity; D001947:Breeding; D002874:Chromosome Mapping / Q000379:methods*; D018703:Founder Effect; D056726:Genetic Association Studies / Q000379:methods*; D014644:Genetic Variation; D005838:Genotype; D006801:Humans; D008126:Lod Score; D008297:Male; D051379:Mice; D010641:Phenotype; D040641:Quantitative Trait Loci; D012984:Software
chemical_list: D001554:Benzene
keywords: DO mice; QTL mapping; Response to toxins; Use case