title: Ligand-receptor binding kinetics in surface plasmon resonance cells: a Monte Carlo analysis.
abstract: Surface plasmon resonance (SPR) chips are widely used to measure association and dissociation rates for the binding kinetics between two species of chemicals, e.g., cell receptors and ligands. It is commonly assumed that ligands are spatially well mixed in the SPR region, and hence a mean-field rate equation description is appropriate. This approximation however ignores the spatial fluctuations as well as temporal correlations induced by multiple local rebinding events, which become prominent for slow diffusion rates and high binding affinities. We report detailed Monte Carlo simulations of ligand binding kinetics in an SPR cell subject to laminar flow. We extract the binding and dissociation rates by means of the techniques frequently employed in experimental analysis that are motivated by the mean-field approximation. We find major discrepancies in a wide parameter regime between the thus extracted rates and the known input simulation values. These results underscore the crucial quantitative importance of spatio-temporal correlations in binary reaction kinetics in SPR cell geometries, and demonstrate the failure of a mean-field analysis of SPR cells in the regime of high Damköhler number [Formula: see text], where the spatio-temporal correlations due to diffusive transport and ligand-receptor rebinding events dominate the dynamics of SPR systems.
authors.affiliation: Department of Physics & Center for Soft Matter and Biological Physics (MC 0435), Robeson Hall, 850 West Campus Drive, Virginia Tech, Blacksburg, VA 24061, USA.
authors.forename: Jacob
authors.initials: J
authors.lastname: Carroll
authors.forename: Matthew
authors.initials: M
authors.lastname: Raum
authors.forename: Kimberly
authors.initials: K
authors.lastname: Forsten-Williams
authors.forename: Uwe C
authors.initials: UC
authors.lastname: Täuber
mesh_terms: D003198:Computer Simulation; D004058:Diffusion; D004867:Equipment Design; D007700:Kinetics; D008024:Ligands; D009010:Monte Carlo Method; D011956:Receptors, Cell Surface / Q000378:metabolism*; D020349:Surface Plasmon Resonance / Q000295:instrumentation
chemical_list: D008024:Ligands; D011956:Receptors, Cell Surface