title: Branched-chain amino acids administration suppresses endurance exercise-related activation of ubiquitin proteasome signaling in trained human skeletal muscle.
abstract: We tested whether post exercise ingestion of branched-chain amino acids (BCAA < 10 g) is sufficient to activate signaling associated with muscle protein synthesis and suppress exercise-induced activation of mechanisms associated with proteolysis in endurance-trained human skeletal muscle. Nine endurance-trained athletes performed a cycling bout with and without BCAA ingestion (0.1 g/kg). Post exercise ACCSer79/222phosphorylation (endogenous marker of AMPK activity) was increased (~3-fold, P < 0.05) in both sessions. No changes were observed in IGF1 mRNA isoform expression or phosphorylation of the key anabolic markers - p70S6K1Thr389and eEF2Thr56- between the sessions. BCAA administration suppressed exercise-induced expression of mTORC1 inhibitor DDIT4 mRNA, eliminated activation of the ubiquitin proteasome system, detected in the control session as decreased FOXO1Ser256phosphorylation (0.83-fold change, P < 0.05) and increased TRIM63 (MURF1) expression (2.4-fold, P < 0.05). Therefore, in endurance-trained human skeletal muscle, post exercise BCAA ingestion partially suppresses exercise-induced expression of PGC-1a mRNA, activation of ubiquitin proteasome signaling, and suppresses DDIT4 mRNA expression.
authors.affiliation: Laboratory of Exercise Physiology, Institute of Biomedical Problems, Russian Academy of Sciences, Khoroshevskoye shosse, 76A, Moscow, 123007, Russia. e.a.lysenko@gmail.com.|Faculty of Fundamental Medicine, M.V. Lomonosov Moscow State University, Lomonosovsky Prospect, 27-1, Moscow, 119192, Russia. e.a.lysenko@gmail.com.
authors.forename: Evgeny A
authors.identifier: http://orcid.org/0000-0002-9504-0237
authors.initials: EA
authors.lastname: Lysenko
authors.affiliation: Laboratory of Exercise Physiology, Institute of Biomedical Problems, Russian Academy of Sciences, Khoroshevskoye shosse, 76A, Moscow, 123007, Russia.
authors.forename: Tatiana F
authors.initials: TF
authors.lastname: Vepkhvadze
authors.affiliation: Faculty of Fundamental Medicine, M.V. Lomonosov Moscow State University, Lomonosovsky Prospect, 27-1, Moscow, 119192, Russia.
authors.forename: Egor M
authors.initials: EM
authors.lastname: Lednev
authors.affiliation: Laboratory of Exercise Physiology, Institute of Biomedical Problems, Russian Academy of Sciences, Khoroshevskoye shosse, 76A, Moscow, 123007, Russia.|Faculty of Fundamental Medicine, M.V. Lomonosov Moscow State University, Lomonosovsky Prospect, 27-1, Moscow, 119192, Russia.
authors.forename: Olga L
authors.initials: OL
authors.lastname: Vinogradova
authors.affiliation: Laboratory of Exercise Physiology, Institute of Biomedical Problems, Russian Academy of Sciences, Khoroshevskoye shosse, 76A, Moscow, 123007, Russia.|Faculty of Fundamental Medicine, M.V. Lomonosov Moscow State University, Lomonosovsky Prospect, 27-1, Moscow, 119192, Russia.
authors.forename: Daniil V
authors.initials: DV
authors.lastname: Popov
mesh_terms: D000293:Adolescent; D000328:Adult; D000597:Amino Acids, Branched-Chain / Q000008:administration & dosage*; D015444:Exercise / Q000502:physiology*; D006801:Humans; D009124:Muscle Proteins / Q000378:metabolism; D018482:Muscle, Skeletal / Q000187:drug effects* / Q000378:metabolism; D010766:Phosphorylation / Q000187:drug effects; D010807:Physical Endurance / Q000187:drug effects* / Q000502:physiology; D046988:Proteasome Endopeptidase Complex / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects*; D025801:Ubiquitin / Q000378:metabolism*; D055815:Young Adult
chemical_list: D000597:Amino Acids, Branched-Chain; D009124:Muscle Proteins; D012333:RNA, Messenger; D025801:Ubiquitin; D046988:Proteasome Endopeptidase Complex
keywords: Branched-chain amino acids; Endurance exercise; Mitochondrial biogenesis; Proteolysis