title: Platelets are responsible for the accumulation of β-amyloid in blood clots inside and around blood vessels in mouse brain after thrombosis.
abstract: Platelets contain beta-amyloid precursor protein (APP) as well as Aβ peptide (Aβ) that can be released upon activation. During thrombosis, platelets are concentrated in clots and activated.
  
  
  
  We used in vivo fluorescent analysis and electron microscopy in mice to determine to what degree platelets are concentrated in clots. We used immunostaining to visualize Aβ after photothrombosis in mouse brains.
  
  
  
  Both in vivo results and electron microscopy revealed that platelets were 300-500 times more concentrated in clots than in non-clotted blood. After thrombosis in control mice, but not in thrombocytopenic animals, Aβ immunofluorescence was present inside blood vessels in the visual cortex and around capillaries in the entorhinal cortex.
  
  
  
  The increased concentration of platelets allows enhanced release of Aβ during thrombosis, suggesting an additional source of Aβ in the brains of Alzheimer's patients that may arise if frequent micro-thrombosis events occur in their brains.
authors.affiliation: Department of Biochemistry, School of Medicine, Universidad Central del Caribe, Bayamon, PR 00960-6032, (P.O. Box 60327), USA. Electronic address: lilia.kucheryavykh@uccaribe.edu.
authors.forename: Lilia Y
authors.initials: LY
authors.lastname: Kucheryavykh
authors.affiliation: School of Medicine, Universidad Central del Caribe, Bayamon, PR 00960-6032, (P.O. Box 60327), USA. Electronic address: jdavilaro41@gmail.com.
authors.forename: Josué
authors.initials: J
authors.lastname: Dávila-Rodríguez
authors.affiliation: Department of Biochemistry, School of Medicine, Universidad Central del Caribe, Bayamon, PR 00960-6032, (P.O. Box 60327), USA. Electronic address: david.rivera11@upr.edu.
authors.forename: David E
authors.initials: DE
authors.lastname: Rivera-Aponte
authors.affiliation: Department of Physics, University of Puerto Rico Rio Piedras, San Juan, PR 00936, USA. Electronic address: lzueva@gmail.com.
authors.forename: Lidia V
authors.initials: LV
authors.lastname: Zueva
authors.affiliation: Department of Anatomy, School of Medicine, Universidad Central del Caribe, Bayamon, PR 00960-6032, (P.O. Box 60327), USA; The Department of Biology, University of Puerto Rico Rio Piedras, San Juan, PR 00936, USA. Electronic address: anthony.washington@upr.edu.
authors.forename: A Valance
authors.initials: AV
authors.lastname: Washington
authors.affiliation: Department of Physiology, School of Medicine, Universidad Central del Caribe, Bayamon, PR 00960-6032, (P.O. Box 60327), USA. Electronic address: psanabriar@gmail.com.
authors.forename: Priscilla
authors.initials: P
authors.lastname: Sanabria
authors.affiliation: Department of Physiology, School of Medicine, Universidad Central del Caribe, Bayamon, PR 00960-6032, (P.O. Box 60327), USA. Electronic address: mikhail.inyushin@uccaribe.edu.
authors.forename: Mikhail Y
authors.initials: MY
authors.lastname: Inyushin
mesh_terms: D016229:Amyloid beta-Peptides / Q000378:metabolism*; D000818:Animals; D001792:Blood Platelets / Q000378:metabolism* / Q000473:pathology; D002561:Cerebrovascular Disorders / Q000378:metabolism* / Q000473:pathology; D004195:Disease Models, Animal; D018728:Entorhinal Cortex / Q000098:blood supply / Q000378:metabolism* / Q000473:pathology; D005260:Female; D007150:Immunohistochemistry; D008297:Male; D008810:Mice, Inbred C57BL; D018613:Microscopy, Confocal; D008854:Microscopy, Electron; D010775:Photic Stimulation; D010976:Platelet Count; D013921:Thrombocytopenia / Q000378:metabolism / Q000473:pathology; D013927:Thrombosis / Q000378:metabolism* / Q000473:pathology; D014793:Visual Cortex / Q000098:blood supply / Q000378:metabolism* / Q000473:pathology
chemical_list: D016229:Amyloid beta-Peptides
keywords: Alzheimer; Aβ-peptide; Platelets; Thrombosis