title: Different treatment schemes and dynamizations of Trypanosoma cruzi biotherapies: what information do they transfer to the organism in infected mice?
abstract: BACKGROUND
  The use of biotherapies in Trypanosoma cruzi infection can provide an understanding about effects of these highly diluted medications.
  
  
  OBJECTIVE
  To evaluate different treatment schemes and dynamizations of biotherapies prepared from blood trypomastigotes (buffy coat) in mice infected with T. cruzi.
  
  
  METHODS
  Swiss mice infected with Y strain of T. cruzi were divided into two experiments. Experiment 1, all treated groups received biotherapy 7dH (10 μL/mL ad libitum) in different treatment schemes: TB7dH- treated 3 days before infection; TBA7dH- treated 3 days before and after infection; TBAe.d.7dH- treated 3 days before infection and every day after infection and IC - infection control. Experiment 2, all treated groups received medication in different dynamizations 3 days before and after infection (10 μL/mL ad libitum): TBA15dH- treated with biotherapy 15dH; TBA16dH- treated with biotherapy 16dH; TBA17dH- treated with biotherapy 17dH; TBAp.chords- treated with biotherapy 'potency chords' and IC - infection control. We evaluated parasitological and clinical parameters.
  
  
  RESULTS
  Experiment 1 showed that different treatment schemes with biotherapy 7dH produced different effects on infection evolution. TBA7dHgroup had the best outcome, with lower parasitemia, higher survival, and better clinical evolution compared to IC. Experiment 2 showed that biotherapy 'potency chords' had effects different from the individual dynamizations that it contained (15dH, 16dH, and 17dH). Animals that had patent parasitemia had delayed emergence of parasites in blood and subsequent increase in parasitemia, but had better clinical evolution compared to IC.
  
  
  CONCLUSIONS
  The effects of T. cruzi biotherapies depend on frequency at which they are administered, dynamization, and host-parasite relationship/individual susceptibility of treated organism. Biotherapy appeared to transfer to infected organism 'antigenic information' related to parasite and 'disease information' related to molecules produced by host's immune response and contained in the buffy coat used to prepare the medication.
authors.affiliation: Department of Basic Health Science, Laboratory of Parasitology, Universidade Estadual de Maringá, Maringá, Paraná, Brazil. Electronic address: fabiana_nabarro@hotmail.com.
authors.forename: Fabiana Nabarro
authors.initials: FN
authors.lastname: Ferraz
authors.affiliation: Department of Basic Health Science, Laboratory of Parasitology, Universidade Estadual de Maringá, Maringá, Paraná, Brazil. Electronic address: karinafranciele@yahoo.com.br.
authors.forename: Franciele Karina
authors.initials: FK
authors.lastname: da Veiga
authors.affiliation: Department of Basic Health Science, Laboratory of Parasitology, Universidade Estadual de Maringá, Maringá, Paraná, Brazil. Electronic address: deniseparasito@gmail.com.
authors.forename: Denise Lessa
authors.initials: DL
authors.lastname: Aleixo
authors.affiliation: Clinic of Homeopathy, Rua Luiz Gama, 485, 87014-110, Maringá, Paraná, Brazil. Electronic address: clinicah@pop.com.br.
authors.forename: Miguel
authors.initials: M
authors.lastname: Spack Júnior
authors.affiliation: Department of Basic Health Science, Laboratory of Parasitology, Universidade Estadual de Maringá, Maringá, Paraná, Brazil. Electronic address: smaraujo@uem.br.
authors.forename: Silvana Marques
authors.initials: SM
authors.lastname: de Araújo
mesh_terms: D000818:Animals; D001691:Biological Therapy / Q000379:methods*; D014355:Chagas Disease / Q000188:drug therapy*; D008297:Male; D051379:Mice; D018512:Parasitemia / Q000188:drug therapy; D011897:Random Allocation; D014349:Trypanosoma cruzi / Q000187:drug effects*
keywords: Animal model; Biotherapy; Clinical evaluation; Homeopathy; Parasitological evaluation; Trypanosoma cruzi