title: Bioactivities of a New Pyrrolidine Alkaloid from the Root Barks of Orixa japonica.
abstract: A new pyrrolidine alkaloid named (Z)-3-(4-hydroxybenzylidene)-4-(4-hydroxyphenyl)-1-methylpyrrolidin-2-one was isolated from the ethanol extract of the root barks ofOrixa japonica.The structure of the new alkaloid was elucidated on the basis of NMR and MS analysis. The compound exhibited larvicidal activity against the fourth instar larvae ofAedes aegypti(LC50= 232.09 μg/mL),Anopheles sinensis(LC50= 49.91 μg/mL), andCulex pipiens pallens(LC50= 161.10 μg/mL). The new alkaloid also possessed nematicidal activity againstBursaphelenchus xylophilus(LC50= 391.50 μg/mL) andMeloidogynein congnita(LC50= 134.51 μg/mL). The results indicate that the crude ethanol extract ofO. japonicaroot barks and its isolated pyrrolidine alkaloid have potential for development into natural larvicides and nematicides.
authors.affiliation: Department of Entomology, China Agricultural University, Haidian District, Beijing 100193, China. liuxinchao@cau.edu.cn.
authors.forename: Xin Chao
authors.initials: XC
authors.lastname: Liu
authors.affiliation: Department of Plant Pathology, China Agricultural University, Haidian District, Beijing 100193, China. dwlai@cau.edu.cn.
authors.forename: Daowan
authors.initials: D
authors.lastname: Lai
authors.affiliation: Department of Entomology, China Agricultural University, Haidian District, Beijing 100193, China. lqzwyz@cau.edu.cn.
authors.forename: Qi Zhi
authors.initials: QZ
authors.lastname: Liu
authors.affiliation: Department of Plant Pathology, China Agricultural University, Haidian District, Beijing 100193, China. lqzhou@cau.edu.cn.
authors.forename: Ligang
authors.initials: L
authors.lastname: Zhou
authors.affiliation: State Key Laboratory of Infectious Disease Prevention and Control, Collaborative Innovation Center of Diagnosis and Treatment of Infectious Diseases, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China. liuqiyong@icdc.cn.
authors.forename: Qiyong
authors.initials: Q
authors.lastname: Liu
authors.affiliation: Department of Entomology, China Agricultural University, Haidian District, Beijing 100193, China. zhilongliu@cau.edu.cn.
authors.forename: Zhi Long
authors.initials: ZL
authors.lastname: Liu
mesh_terms: D000330:Aedes / Q000187:drug effects; D000470:Alkaloids / Q000494:pharmacology*; D000818:Animals; D000852:Anopheles / Q000187:drug effects; D000969:Antinematodal Agents / Q000494:pharmacology*; D003465:Culex / Q000187:drug effects; D007306:Insecticides / Q000494:pharmacology*; D007814:Larva / Q000187:drug effects; D009348:Nematoda / Q000187:drug effects; D024301:Plant Bark / Q000737:chemistry*; D010936:Plant Extracts / Q000494:pharmacology; D018517:Plant Roots / Q000737:chemistry; D011759:Pyrrolidines / Q000494:pharmacology*; D011760:Pyrrolidinones / Q000302:isolation & purification / Q000494:pharmacology*; D027821:Rutaceae / Q000737:chemistry*
chemical_list: C000617674:(Z)-3-(4-hydroxybenzylidene)-4-(4-hydroxyphenyl)-1-methylpyrrolidin-2-one; D000470:Alkaloids; D000969:Antinematodal Agents; D007306:Insecticides; D010936:Plant Extracts; D011759:Pyrrolidines; D011760:Pyrrolidinones; C032519:pyrrolidine
keywords: Orixa japonica; larvicidal activity; mosquito; nematicidal activity; pyrrolidine alkaloid